LOCUS       DATNSN010000001        79741 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.001, whole genome shotgun sequence.
ACCESSION   DATNSN010000001 DATNSN010000000
VERSION     DATNSN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 79741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79741
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.001"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            99..1730
                     /locus_tag="VAH76_000001"
     CDS             99..1730
                     /locus_tag="VAH76_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEO1384665.1"
                     /translation="MHPNYYLSPLAVAIALGIASPVKAADPIPLQKSSFSEVTQKFQL
                     TLPGVMKGAVVSTNSLQFIRQHTDGNKVTHVRMQQQYAGFPVFGGYAILHSKNATPSL
                     ATAKSDVKMNGVIYDGLQAELGQPKPSFVKNASMALQQFKDKYANKQVSEDQVTPMIY
                     IDEKHQAHWAYKVSVLVIHDDRIPERPTAIIDAETNKPFVQWDDVKTEKVQAKGMGFG
                     GNRKIGEYQFGKDLPLLEITRDSSVEMCFMENTDVKVVDMGHKYYSNNKPMQFTCKET
                     PDTQSTKTYYTGYSADGYDRDNGAASPTNDALYAGYVIKHMYHDWYGVEALTKSDGSP
                     MQLVMRVHYGQGYENAYWDGKQMTFGDGDTMMYPLVSLGVGGHEVSHGFTEQHSGLEY
                     FGQSGGMNESFSDMAAQAAEYYSVGKNSWQIGPEIMKEDSGYDALRYMDKPSRDGMSI
                     DVADDYYGGLDVHYSSGVYNHLFYILANQPNWNLRMAFDVMVKANMDYWTPYSTFDEG
                     GCGMLSAAKDLGYNLDDIKKSLSEVTINYQSCYVD"
     gene            complement(1732..2580)
                     /locus_tag="VAH76_000002"
     CDS             complement(1732..2580)
                     /locus_tag="VAH76_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1384666.1"
                     /translation="MKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDDIAE
                     HLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGA
                     CLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKF
                     EHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCL
                     TEIKAQTYLIWASKGFSFDSDKMKARIQAVKNIKIEKLDGGHHIHMEKPEVISRLLAE
                     FYQSIK"
     gene            3049..3804
                     /locus_tag="VAH76_000003"
     CDS             3049..3804
                     /locus_tag="VAH76_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO1384667.1"
                     /translation="MQPEVRSVSLITYNIHKGFGVGAVRFLLPEMRDAIAGLNPDFVF
                     LQEVQGKHRKREKRIVSWPDSPQCEYIAENIWPHYVYAKNAVYQSGHHGNAILSKYPF
                     ESFESINLSNMNRASRGILHTQLKLDNNIIHLLCVHLGLFKAERTEQCKALIQRIKDV
                     VPKNEPLIMAGDFNDWRHVISKILAENLGIEEAFVALEGQHARSFPAIKPALCVDRVY
                     FRGMKVQEVACLQGKPWRMLSDHLPLYARFELL"
     gene            complement(3983..4993)
                     /locus_tag="VAH76_000004"
     CDS             complement(3983..4993)
                     /locus_tag="VAH76_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HEO1384668.1"
                     /translation="MSYEELSNTMEQMLHEGKGILAADESNSTIGKRFASINTENTEE
                     NRRDYRLLLATTPDLEQYINGVILFEETFTQTDEHGTPIPELFANKGIIPGIKVDKGL
                     VDLANTEHEKVTQGLDGLTERLLHFKKLGAKFAKWRNVYSISEYTPSLTAIKTGAENL
                     GRYAAACQSVGIVPIVEPEVLMDGSHSIEHCAEVCEMVLHELFHTLFIHQVELEHIIL
                     KPSMVTCGKEVTPFSDPEEVADYTISVFRNNVPASVPTINFLSGGQTPQQATANLNAI
                     NQIGHQPWLLSFSFGRALQEDCLKEWCGNKANIAKAQEALIKRARLNSMACLGEYQND
                     ME"
     gene            complement(5143..5877)
                     /locus_tag="VAH76_000005"
     CDS             complement(5143..5877)
                     /locus_tag="VAH76_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEO1384669.1"
                     /translation="MQINTFPITLEESFMISPKVKHFIFNCELSPPFEYLPGQFITIH
                     FEHDGKNLKRSYSIANEPKKDNRIEFAAGYFEGGPGTELLYNLKPGDVIHINGPFGRL
                     IFKDETPGRYILVATSTGTTPYRAMLNELGQRIEKHPDLQVVILQGVQRSEEILYPDD
                     FQAFAKKYPQASFRPYLSRVQKQDLKDNEYSGYVQHAFPELNLNPTRDMVYLCGNPGM
                     IDEAFNYLKEHGFPMQHIVREKYISR"
     gene            5961..6503
                     /gene="dotV"
                     /locus_tag="VAH76_000006"
     CDS             5961..6503
                     /gene="dotV"
                     /locus_tag="VAH76_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein DotV"
                     /protein_id="HEO1384670.1"
                     /translation="MNTIDLITILGNISQSLYPVQKLITGGAYILGVLFFLTAIGKLK
                     KIGDHRAQSSSQEKMFTPMMYLLVGALLIYLPSGMDLMANTAFGVGNVLTYSNYNPAN
                     IYSSMGFLIRTAGVLWFIRGCVLVAHSSQPGTQQGLKGLVFLIAGVFAMNFDNTIAMI
                     NSALDYFVKATLSVKAAAGF"
     gene            complement(6647..6931)
                     /locus_tag="VAH76_000007"
     CDS             complement(6647..6931)
                     /locus_tag="VAH76_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEO1384671.1"
                     /translation="MSNRKNKTIVIEGVTSQGKTFRPSDWAERMSGSLAVFKNSRIYY
                     SPLLQPSVNSEGYKCVLLDPKLKESSPQVYQAIMDFAKANNLKICGEEDL"
     gene            7161..7898
                     /gene="pssA"
                     /locus_tag="VAH76_000008"
     CDS             7161..7898
                     /gene="pssA"
                     /locus_tag="VAH76_000008"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEO1384672.1"
                     /translation="MNKENHSGIYLLPNLLTTVSLFAAFYSIVASTKGQYEAAIVAMF
                     IGMIADSLDGRIARLTNTQTEFGAQYDSLSDMVTFGVAPSLLLYNLTLHQLGKFGWLV
                     AFIYTAAVALRLARFNTQIGKSDKRYFQGLPCPPAAAVMASFAWICFQNEWANLIVSL
                     FTAVLALIVSVLMVSNLRYYSFKEVDYKGKVPFLYVLVMILLFVAIAANPAIVLFASS
                     GIYALSGPMLTVISLHKMRKQRRNMSK"
     gene            complement(8140..8409)
                     /locus_tag="VAH76_000009"
     CDS             complement(8140..8409)
                     /locus_tag="VAH76_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEO1384673.1"
                     /translation="MIKTKIKIINRLGLHARASAKFVSTAAKFQSHIDVTKDTQTVNG
                     KSIMGVMMLAANKGSELTLEIDGPDEERMNEALVELINNRFGESE"
     gene            complement(8600..8899)
                     /gene="hpf"
                     /locus_tag="VAH76_000010"
     CDS             complement(8600..8899)
                     /gene="hpf"
                     /locus_tag="VAH76_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HEO1384674.1"
                     /translation="MQINITGHGMDVTPALRAFTEEKFDKLERHFDHIRAINVVFDVE
                     KVRQIAEATVLVTKGELHASSESEDMYAAIDTLVDKLNRQLIKHKEKLLAHRDRD"
     gene            complement(8926..10320)
                     /locus_tag="VAH76_000011"
     CDS             complement(8926..10320)
                     /locus_tag="VAH76_000011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEO1384675.1"
                     /translation="MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESN
                     PLLEATPVEEKEDVQINTNQNQDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTT
                     NLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
                     IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNY
                     RQLMKNHQISEKTIHEILQVIHKLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLN
                     QDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQSRQETLLKVATCIM
                     EYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSH
                     VNTDSGGECSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREA
                     MGIPPSNERKVISS"
     gene            complement(10532..10696)
                     /gene="rpmG"
                     /locus_tag="VAH76_000012"
     CDS             complement(10532..10696)
                     /gene="rpmG"
                     /locus_tag="VAH76_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO1384676.1"
                     /translation="MAAVTIKVKMESTAGTGYYKTTTKNPRNHPEKMELMMYDPKVRK
                     HVLFKEKKVK"
     gene            complement(10711..10947)
                     /gene="rpmB"
                     /locus_tag="VAH76_000013"
     CDS             complement(10711..10947)
                     /gene="rpmB"
                     /locus_tag="VAH76_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO1384677.1"
                     /translation="MSRVCQVTGKRPMVGHKVSHANNKTKRRFLPNIQNHKFWVEEEN
                     RFVTLRLSTKGMRIIDKLGIKAVLDKIRAKGEKI"
     gene            11216..11893
                     /gene="trmB"
                     /locus_tag="VAH76_000014"
     CDS             11216..11893
                     /gene="trmB"
                     /locus_tag="VAH76_000014"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO1384678.1"
                     /translation="MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAK
                     EFGRHDADTIVEIGFGMGTSLFTMAMNNPQCNYLGIEVHKAGVGSLVADLHEHQITNV
                     RVVVHDAVDVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
                     FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFEQRGERLGHGVW
                     DLVFIKK"
     gene            11905..13029
                     /locus_tag="VAH76_000015"
     CDS             11905..13029
                     /locus_tag="VAH76_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO1384679.1"
                     /translation="MIKHAILISGLIVSFLASAAEMSFLAQLTEVPGASGYEKNVRHL
                     VQKQWQSYMAELKIDGMGNLIGQRKDNQKGPKLLFMAHLDETGFMVESITPDGFLKVI
                     PLGGIANSVIYAQRWTVSTPKGPVLAYSGMDSPHLLGDKKMSGSPDINALFLDIGAEN
                     KEQAQKEFAIQPGLEVTPVSEFSQLSKNRFLGKALDDRLGLAVISDALRAVKQQPNQL
                     YLAATVQEEVGMRGASTVYKATHPDIAINVEVGIADDYPMLLADRKGRIGLGKGPSLF
                     VYDKSMIPNQELINWIMELANKNNIPLQLEVEPGYGEDGSKVQASGMGVPVVNIGIPI
                     RYAHQHAGIFDKRDYDNTVKLLRLIAENLNQEVVDKIKKS"
     gene            13107..14594
                     /gene="ankC"
                     /locus_tag="VAH76_000016"
     CDS             13107..14594
                     /gene="ankC"
                     /locus_tag="VAH76_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkC/LegA12"
                     /protein_id="HEO1384680.1"
                     /translation="MDFVSEMNKILQMELEQFKEFIQKKLEEDKNYLEKLFWLPNGSQ
                     MTVLNYLIEQYSEPKLGIDDANRDLLAKIDFVLHEAKDVNVGEPLHQAIVAGKISLAL
                     HLLGVDENNFTPGESEKTDVLSILSKVKKKIEESFNHVKRCFFDVDKRDGYGRTLLSL
                     ALDAKRQELLIAILARNPIVHATTLRSSAYVPFQPIHQAVVLDYAEGITLLASMGAQL
                     TNPLGSMRDTPVILAARLGKINALAALLELPTQSLSLESENNHLFEDKQTGHTAVEEL
                     CERMANENDKAEALRGIAMLICRGAEPPRNEKMRNLLSSNRVAFLKAVSTYLADKPQL
                     VDAFVERCHLRESALHNIVYADHSWGSSIRHLLGVPSEAALIVEELVTRKYSDPSFAS
                     ENVSSLSTTATENLRSERNPLKLYAEFVRRYTQAYDSQMITNRWSTMRWMIAEGNCDW
                     DTVVRYSKNHPTSRTRIVLNEMFNPIPRVHEDIESQQNSPRVVLK"
     gene            14803..15945
                     /gene="hflK"
                     /locus_tag="VAH76_000017"
     CDS             14803..15945
                     /gene="hflK"
                     /locus_tag="VAH76_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEO1384681.1"
                     /translation="MGWNEPEKGKDPWSGKNQPPDLDEALKRIQDKLKKTFFGGTGKS
                     NNNSSGGSSGGLLAVTVLLIAFILWALSGIFIVDPAEQAVILRFGKYAETVGPGPHWI
                     PRFISSKIVMNVDRVLDYSYSAQMLTSDENLVSVSLAVQYRINDLSEYLFNVANPEES
                     LQQATSSALRQVVGTTTLDQIITEGREVWGGRVQETLTKTLESYKTGILIVNVSPQPA
                     RAPESVQDAFDDAIKAQEDEKRFKEQAYAYAAKVVPIAEGKASRIQQEAEAYSKQVVL
                     RAQGEVAEFLALLPQYNAAPQVTAKRMYLEAMQKVMNKSSTIIVDSKAGNLLYLPLDK
                     LLGNQYVPQSGNLKAIKNGTNVSNEEDNLILSGRDLTRPTYSQGRD"
     gene            15948..16862
                     /gene="hflC"
                     /locus_tag="VAH76_000018"
     CDS             15948..16862
                     /gene="hflC"
                     /locus_tag="VAH76_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEO1384682.1"
                     /translation="MNAFKTTLGVLIFLVLVLLFTTMFTVTQGQQGIILRLGRLVKDP
                     QTDAVKVLNPGLHFKTPFIESVRIFDTRIQTMDIKSTRIVTKEKKDVMVDYYVKWRIS
                     DLAQYFKSTGGNEFKAETLLEQQLNTLLRAQFGKRTISDAVSGGRDDVMEILRNAAEK
                     QAGELGIKVVDVRIKGIELPSNTSNAIYQRMRADMQKIANRHRADGQAAAEQIQAKAD
                     ADVTVLLAKTNSNAQRIRAVGEAEAAAIYSKAYTQNPDFFALYKSLLAYEASFHSKKD
                     ILILDQSSSFFDYFKQAMPKNDGTAAKK"
     gene            16997..18292
                     /locus_tag="VAH76_000019"
     CDS             16997..18292
                     /locus_tag="VAH76_000019"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO1384683.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTL
                     KINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISL
                     ACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
                     KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGEN
                     ILFEGAQGVYLDIDHGTYPYVTSSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGG
                     GPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLD
                     VLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDL
                     PANARAYLKRIEEILGIPIDMLSTGPERDSTITLRGPFL"
     gene            18512..19081
                     /locus_tag="VAH76_000020"
     CDS             18512..19081
                     /locus_tag="VAH76_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEO1384684.1"
                     /translation="MEYLTPSGLIPFITELKALNILQQDDNTNIALVSEILARWVVDK
                     EWLDEKFYYVNPEVGFNSWLIHEEKDHTLAVNIVAWQSGREITPHDHQTWGVVGSIIG
                     VEKNYFWKRMDDGSKPGYAEIIREKTAVIRPAGGVIGFLPNDIHSVINESDDVAISLH
                     VYGKNLNYTGRHQYDPVNNLMQPFIVNYS"
     gene            complement(19281..19739)
                     /locus_tag="VAH76_000021"
     CDS             complement(19281..19739)
                     /locus_tag="VAH76_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO1384685.1"
                     /translation="MTHDAFLDDLILSIVQTESIAEQSDLQNSLRKRGYEIPQATLSR
                     RLKKMKIAKVSGYYKIIDYNQPGLPLVLNVQISDFGLIILQTHPGNANSLAYYIDRKY
                     VSFSIKDSSNSGILGTIAGDDTVLLIIKSKSDQQKVLDILMKEFPYLFAS"
     gene            19870..20604
                     /locus_tag="VAH76_000022"
     CDS             19870..20604
                     /locus_tag="VAH76_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO1384686.1"
                     /translation="MRKVFSLLLISSIIVISSCSEQKNQNTLRFSTSAEYPPFEYIEN
                     GEIKGFDIDLAKLIAKELGKEAVFDNMQFSAVLPAVNSGQDDVAIATITITEERKKNF
                     DFSKPYYFEGMAAVFDNSQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPKAEITALDN
                     NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLKKGSPLRDE
                     INSALENLKAKGEIQKLESIWIKGSI"
     gene            20601..21248
                     /locus_tag="VAH76_000023"
     CDS             20601..21248
                     /locus_tag="VAH76_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO1384687.1"
                     /translation="MTEIVQNLFFIGKGIVLTMQLLTGGMLIGVLLGTLLSICRHQGV
                     ACFVINRFISILRGTPLILQLSFIYFAVPVLLGMRPSILVAGIVAFGLNSSAYFAEIL
                     RSGIESLPKGQFEAALTLQIPTFYLWKDIILPQVIKNIFPAMINEMIALLKETALIST
                     IGGMDLMRQAQTLAAQQFTYFMPLCITGFYYYSLVLLLEALGKKIEKRGHYAASH"
     gene            21232..21900
                     /locus_tag="VAH76_000024"
     CDS             21232..21900
                     /locus_tag="VAH76_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO1384688.1"
                     /translation="MLQVINASKKIGNAQVLNEINLSIQANSIVGLVGPSGSGKTTLL
                     RCIQQLESIDSGSIHCEGKSGFMFQDFQLFPHMTVLNNLIYAPYLHDKTADHMANAWA
                     LLAMLGLSDKAHSYPQGLSGGQKQRVALARSLMMKPDLLLCDEPTSGLDMATTNDVIA
                     LLNTVKTLKITMLIASHDLFFLSNIAERLLVVKQGQLIADFKTQEVSDPILYLQQFYQ
                     GEIL"
     gene            21897..23114
                     /locus_tag="VAH76_000025"
     CDS             21897..23114
                     /locus_tag="VAH76_000025"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO1384689.1"
                     /translation="MKKVIKKIALAYSGGLDTSIMIPWLKEHYEHAEVIAVICDLGQQ
                     EDLDTIKNKALKSGASKAYVVDVKNEFAIQYLWPLVKSGALYEDQYILGTISRPLIAQ
                     KLVEIALTEQVNAVAHGATGKGNDQVRFEYSIKALAPQLEIIAPWRTWDIKSRQEAIV
                     YAKAHGIEVPVTPKAPYSRDHNIWYISHEGGVLEDPSQEMPDDVLLMTAPVSQTPDEE
                     EVVVLDFKKGVPVALNGQELSPVDLLNSLNQKAGQHGIGVADIVENRLVGMKIRGIYE
                     APAAAVLYKAHKLLESLCLTRSTLHLKQSLQQTYANLVYEGRWFSQTKQALDAFIDVT
                     QQHATGCVKLKLFKGNIIPAGMHSPYSLHHPELATFEEDNVYNQKDAEGFINLFSLSA
                     KIYSQVHQEGNYD"
     gene            23107..24342
                     /gene="argH"
                     /locus_tag="VAH76_000026"
     CDS             23107..24342
                     /gene="argH"
                     /locus_tag="VAH76_000026"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO1384690.1"
                     /translation="MTNKTWGGRFKKSLDSGVNQFNASLSFDHVLFDQDINGSQVHVK
                     QLAKQKILTEVESQAIYSALEEIRSEIKQGQYSFNVRDEEDIHMFIEQLLIQKIGDLG
                     KKLHTGRSRNDQVALDLRLYTRDKGCLINELLTRLIDCLDDLTSKHQQDLMPGYTHLQ
                     QAQPVTLGAYFNAYQCMFGRDKSRLEDWFKRMNYSPLGAGALAGSTLPLDREWVAESL
                     GFAGIIPNTLDAVSDRDFVIELCSVAAMIMMHLSRLCEDLILWSTQEFNFVILDDAFA
                     TGSSLMPNKKNPDVPELIRGKSGRVYGHLMAILTVMKGLPLAYNKDMQEDKEGLFDTI
                     NTIIACLQMITPFLQSLTFNTLLMKTKAQSGYLDATAILESLVMKGMPFRDAHHQVGA
                     WIAEAIEKQCSLNELLKGG"
     gene            24345..25460
                     /gene="argF"
                     /locus_tag="VAH76_000027"
     CDS             24345..25460
                     /gene="argF"
                     /locus_tag="VAH76_000027"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO1384691.1"
                     /translation="MSFSTSTNLTPIETQDDDLEKIQSQNNPSAINEVKTNTFVINLQ
                     QAIFGLETGSEAVDFDSHKNTGFPFPAGEYKPRHFLTGTELAREEIQFILSQARSLKQ
                     NPGLYNQALANKSLVMIFEKPSFRTRLSFALAIQSMGGIVVESIANSRKQETPADMAR
                     VLNGYADFVMVRTHEDSFLQEMAEYSKIPIINGLSALHHPCQTLADLLSLEEQFGALE
                     GLTLTYIGDGNNILHSLMLLAPQLGVTVHYCCPKSNQPNEAIIKEAQSKTGKQMIYCF
                     DKPEEAVKQADAVYTDVWTSMGFDNPEAGKIFKGFQVNETLMSHAKPDSVFMHCMPME
                     RGKEVSATLPDSASSIIFRQSENRMHVQKALMLFLDQ"
     gene            complement(25550..27025)
                     /locus_tag="VAH76_000028"
     CDS             complement(25550..27025)
                     /locus_tag="VAH76_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO1384692.1"
                     /translation="MNITRLLAFFTSFFLLCPIYASVDSYFSHIKKDSSALYAFFKTM
                     PKGGELHYHLAGGAYPETMLALAANGNYCLDKHSFAVSKDSSHCEGVNVKEVFNNPEL
                     YSNIIKDWSMKDFIPGKESGHDHFFNGFVKYMPIVFDYRPQLLADIVQRAAQQKEQYM
                     EIMIIPDNAHSLTYGDLIKDIPSYDQKRHLLLANKDFQSNIKNTVLESDRILTKASQE
                     LGCKDNPSSDSCHVKIKFIYYVLREQPVDNVFAQALNAFEAVSKSKGNLIGVNLVQPE
                     DGIISLRDYHKHMSIFEYLHRIYPNVPITLHAGELSPQAVTPENLSNHIRDALLIGHA
                     QRIGHGVDIGYENNAEDTLKYMASHHIPVEINLISNLKILNISGRNHPLNYYLTHNVP
                     VVLSTDDEGVLRTDLTQQYVEAAKEHGLNYQQLKQINRNTLTYSFLPGKSIWANADKA
                     ELIPDCHDLGSKSCQKFIEHNEKAKLQWSLEQQLIAFEKNY"
     gene            27200..28315
                     /locus_tag="VAH76_000029"
     CDS             27200..28315
                     /locus_tag="VAH76_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO1384693.1"
                     /translation="MPYFYTRSAPLFFVFVIALIFINFSYAHEFQTDSKNKHKVDVHI
                     GIYAPFSSHSAFIGRNILAAMEMGSERIQSSDIHYSFYTLDQLPKNKNAGLVLQKFID
                     IHHINVLVTGGKETGLIAAPIAKKNNLIHFSLTNNTEVADGRNNFIVWSPAYDQAKVL
                     VNELKRQQVHHLGIITANQPSSTALTHSVIKQLQADSHIDVAASEYFEPGNKDFSRLT
                     NRIKRKNADMYLVMASPEDIKLIQEQMEADKQHVPITSIVERATPEVVKVFEGQWYVD
                     SHEMQPDFINEFKKNYLNYPVTEAGYAFDLFQLLNKSIVMAIKTQGSPTSQEVVHQLH
                     DIANGTGVMGTMVLDSKGSLFTKSEVKTVKNGEIHTV"
     gene            complement(28387..29724)
                     /locus_tag="VAH76_000030"
     CDS             complement(28387..29724)
                     /locus_tag="VAH76_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO1384694.1"
                     /translation="MVIKKLFSSTLALVYALTLMLPLTAFSAEETNSNYSNTKRIPLE
                     DVQRFSNAIGEIKKYYVKPVDDKELFDNAIRGMLTGLDPHSSYLNEEEFKELQTSTSG
                     EFGGLGIEVTMEEGVVKVITPLVDTPAFKAGIKSGDYIIKLGKESVQGLSLKDAVNLM
                     RGKPGTTIELTILRKGVNKPLTFDLIREVIQIKSVKSKMLSEGYGYIRLTQFQALTGK
                     DMIKAIEQLKQQAGGKLKGLVLDLRNNPGGLLDSAIQVSDAFLGNDKAGKQEMIVYTE
                     GRLPGSKFTALANPGDVLDNAPIVVLINNGSASASEIVAGALKDNKRAIILGTKSFGK
                     GSVQTVLPLDGKTGIKLTTALYYTPSGVSIQAKGIIPDIVVEEVDVPKNAVKKDTLAG
                     FTEADLNGHLINKDNSENTESKNNQIQTGDLIHEDYQLYAALTVLEGMALANR"
     gene            complement(29805..30947)
                     /locus_tag="VAH76_000031"
     CDS             complement(29805..30947)
                     /locus_tag="VAH76_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO1384695.1"
                     /translation="MRMSSLSNYLSNAPLGLALMVILFFCNGLEAKAAPNSITQAQTK
                     LKQLDAKINKLKQVLANAQDKRTVLNQELGSTEKHIGEGIRKLRIIQANMSAKEKSIA
                     ELQGKINHLNERLQAQQEMLANHVRARYQMGEYQPLKWLINQDEPFKINRILTYYQYI
                     IRSRQQLIDEIARTRQNLNDSKTKLGNELAENKQLQFKLSQNQKQLEQNKRYHSTLIQ
                     SIDNDIQNNQQSLKDFQKDRDNLSHLMKSLSQQSVIQSGKPFTGMRKKLPLPVQTTHR
                     SLRRMNQGVTFFADEGSIVTAVYPGKVVFSDWLKGYGLLLIIDHGQGFMTLYAHNQSL
                     FKRKGQIVQQNEQVASVGHTGGIKQNGLYFEIRQRGKAVNPLDWLS"
     gene            complement(30934..32478)
                     /locus_tag="VAH76_000032"
     CDS             complement(30934..32478)
                     /locus_tag="VAH76_000032"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEO1384696.1"
                     /translation="MSHNAPLVLMILDGWGYNENDRYNAIAKANTPQWDEWWQTCPHI
                     LLKASGLPVGLPDEQMGNSEVGHMHIGAGRVIQQDFTRINEAINNGKFAKNAVFHEVI
                     DQLKKTEKSLHIMGLLSPGGVHSHEQHLFALLALCNQKKFRSVHLHLFLDGRDTPPQS
                     ALDSLKCLNEELVKHPVATINSICGRYYAMDRDKRWERVEPVYNLLTQGKSEHQFPDA
                     ETAIHFYYKNKISDEFVPPTLIGKEHSIQDGDAVLFFNFRADRARQLTSTFLDPLFKG
                     FERKTLPKLSYFVSMTQYDKNLFTTTAFPPVPLNNTLGEVLSSHGLSQLRIAETEKYA
                     HVTFFFNGGCESVFTNEERIMVPSPQVATYDLQPEMSAPELTKTLIAAINSRDYHVII
                     CNYANADMVGHTGNFEATVQAIECLDQCMHQVWQALKNNGGKLLITADHGNAEEMFSE
                     ATNQAHTAHTSEPVPFLYVGGGWHFTHAEGSLIDIAPSLLALLGITPPPEMTGRILLE
                     KNHAHV"
     gene            complement(32581..32877)
                     /locus_tag="VAH76_000033"
     CDS             complement(32581..32877)
                     /locus_tag="VAH76_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384697.1"
                     /translation="MSRDTKNLILKAIILFVSLTALVNHAMAFNLLGIGPNPKNLPKD
                     NDVRATFNHCKSCDKSTTPPLIITTPVPIIYYPGCRPIQSCDAYGHCEQKEDCY"
     gene            complement(32954..34225)
                     /locus_tag="VAH76_000034"
     CDS             complement(32954..34225)
                     /locus_tag="VAH76_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEO1384698.1"
                     /translation="MKKSIHRLIKPRLFAMSISLLAFSSITYGESNGSFALSEHWSMG
                     QQIKLRFDINEIPQSGVVLHLKNGLALTAGDIISLGDLYGIVGKPISHGLTKLEKQTR
                     FKEVFNSFARNAIAVSEVEDLNSVIRAEMREVESGIAKGETAEAIYKRIGNEVGRQIN
                     CITGGGCNSLTWWLNPGRYLKLAMENYDHFTPNNLIAYKSGHHVALQQALKAKRTGKR
                     SDLEIAYAMDAFACHYLSDHFAAGHLRTPREELSAIITPTVLGALLSNYMHNEENKYG
                     IHVTNNLGEQWMVYGDYSYFNPFNQTNQQMLLKALQASADEIFEAYYSGVLPEKNQIE
                     DMIPHITKLNDENNMDIAPMFYWDKENKQLLRRTNLTNPYDRHWTNSWWGWSTLLLLK
                     TQYGITSTVQLSLSKYLSQYVPEELQDNPLV"
     gene            34575..35291
                     /gene="uppS"
                     /locus_tag="VAH76_000035"
     CDS             34575..35291
                     /gene="uppS"
                     /locus_tag="VAH76_000035"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HEO1384699.1"
                     /translation="MNHKLPQHIAVVMDGNGRWAESRGLPRVEGHKAGLDSVKKIINC
                     CLEKKISCLSLFAFSSENWSRPVTEVNFLMELFLEALRKEIDDLNQHGIRLKFTGDRE
                     PLSQVLQKQMCDAEALTKNNQQLILNVVVNYGGKWDIVTAARKLIRTVLDGKLAYDEI
                     NEAVFAQFLDTNGMPEPDLFIRTSGELRISNFFLWQLAYTELYFTDVHWPDFNEHEFE
                     LALTSFARRARRFGQISQSE"
     gene            35302..36099
                     /locus_tag="VAH76_000036"
     CDS             35302..36099
                     /locus_tag="VAH76_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO1384700.1"
                     /translation="MFMQRLITTLILAPLVLLAIFYAPSWILWGIVLVILLLAGLECF
                     KLIPVDNLTVQACFLIFLLMCFWASGYFYPYWLSVGLLVWLLNGLAILTYPRSQNYWG
                     YPIVVAIASLILLPLFTQSLIQISFLQQGKELLVYLLFLIWASDIGAYLAGKQWGKHK
                     LIPKVSPGKSWEGVTGGVILAMIVATLGYYYFIPYSIYIWFGLALSTVVISIFGDLFI
                     SILKRRCQLKDTGNIIPGHGGILDRLDSLIAALPWFYFGLTYIPLGI"
     gene            36132..37484
                     /gene="rseP"
                     /locus_tag="VAH76_000037"
     CDS             36132..37484
                     /gene="rseP"
                     /locus_tag="VAH76_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO1384701.1"
                     /translation="MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKI
                     LARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGP
                     LFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWR
                     DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIP
                     TIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKR
                     QGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTIQ
                     LTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALN
                     LLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
                     "
     gene            37655..39967
                     /gene="bamA"
                     /locus_tag="VAH76_000038"
     CDS             37655..39967
                     /gene="bamA"
                     /locus_tag="VAH76_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEO1384702.1"
                     /translation="MKKVSNKLILGVCCSTLLAWSSQSFSSDTFIVKGIRVNGLQRVS
                     TGTVLNYMPVQVGEEISSSSTAQIIRALYETGFFQSVSLERQGNVLVVNVIERATIGS
                     ITVVGNKEIPSDKMKAFLKEMGLVKGRVFQRSSLERLEKELKQAYTARGKYNSRIETK
                     VTPLTENRVAISITVSEGRVSRIKEIKIIGNHDFKANELLPELTLSTSNLFTYFTKKD
                     QYSKPGMDASLEALRSFYLDRGYLKFNVVSSQVLLSPDKKDVYINIHIEEGPQYHFSG
                     YDVVGKTILPKEKIDSLIQVKKGDVFSRKKVTESISAIGLALGDVGYGFPAINAEPRI
                     DENNKTVFITFVVQPGRHVYVRRINFHGNTKTGDYVLRNVIRQDEGGLLSLHNIKESE
                     RQLRMLGYLKNIDVKTTPVPGTNNQVDLDVNVEEAPSAEASASMGYGTNGYQFNASVN
                     QRNFMGSGRSMGAAFNASQWGQDYSFNYYNPFYTDTGVGRGGSLYYSRIDPKNLNVST
                     YSSNRYGGDISYNFPLGEKSSFQLGYGYQDINIKSVGYVLPIINFVALNGNHFQEIRL
                     TSGWSRNSYDQMPYPNQGFNQQAIAMVALPATSQSLSYYKSSYQAHLYYPLTRGWIFS
                     VLGNVGYGNTFDNFGLPFFENYYAGGPVQPGQVRGYDSYSLGPQDNFGNAMGANFLVN
                     GSVGLILPYPLSRDNVRTTIFADAGNVFASGTPPALRGTPAGPMRYSAGVSLEWRSPF
                     GPLSFSLAKALNPQPLDQTQLFQFALSSGF"
     gene            40081..40581
                     /locus_tag="VAH76_000039"
     CDS             40081..40581
                     /locus_tag="VAH76_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEO1384703.1"
                     /translation="MKRLGAVLVALFLGLFGTTVFADWAKIGVVDLQKIMQTSNQMKE
                     IQQKLEKEFKPRRDKLVAMEASLKSDMEKFKRDSAIMSASQKKELEKKIVASQQQFER
                     DGQQYQQELSTAHNEAMEGLYNKVRTAITKIAKDEKYDIIVQKEAAPFSSESLDVTDK
                     VIKAIN"
     gene            40595..41614
                     /gene="lpxD"
                     /locus_tag="VAH76_000040"
     CDS             40595..41614
                     /gene="lpxD"
                     /locus_tag="VAH76_000040"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEO1384704.1"
                     /translation="MLTLEELANYLNGVWHGNANHAIFSLSSLARASSKDLAYFDNPL
                     LQSVLNSTRAGAVLLKPEHKDWCPVNCIVVPNPSEAMFKAAKSLPHLATNVCGIHPTA
                     QIHKSAQIGQYVSVGANSVIGENVQLDDYVTIGSNTTIESSVLIGRGSQLGSGAIIHS
                     GTVLGQSVIIDSGCIVGAAPFNCYKEHGVWQQAPNFGGVVIGQRTQIGANTVIHRGSI
                     GDTYLGDGVCIDSLVLIAHDVYIGNNTAIAGSAAIGALVQIGSDCIIGGASCLAANIR
                     LTNDVVITGMSTVTKSIARPGIYSSGTTVHDHRKWRRNTARFRHLDDYITKLITIEKK
                     LNNNN"
     gene            41732..42184
                     /gene="fabZ"
                     /locus_tag="VAH76_000041"
     CDS             41732..42184
                     /gene="fabZ"
                     /locus_tag="VAH76_000041"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO1384705.1"
                     /translation="MNESIDINQIFTLLPHRYPFILVDRVIDYKVMEYLIAIKNVTIN
                     ENFFTGHFPGNPIMPGVLMLEALAQACGILANLSRQPKEGYEFLHYFAGIDNARFKQV
                     VIPGDQLRLEVRLAGQKRDFWRMHGEAYIGDKLACSADLLSAAKEIKK"
     gene            42181..42951
                     /gene="lpxA"
                     /locus_tag="VAH76_000042"
     CDS             42181..42951
                     /gene="lpxA"
                     /locus_tag="VAH76_000042"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEO1384706.1"
                     /translation="MIDERAMIHPSAKLASGVSIGPGTVIGADVEIGENTWIGPHVVI
                     EGPTVIGKNNKIFQFASVGDEPQDITYKGEPTRLEIGDNNVIREYCMISRGTVKGGGV
                     TRIGNSNYLMAYSHIGHDCMVGNHIIMVNYAALSGHVTINDYAIIGPYAAVHQFCQVG
                     AYAFIARATYVTKDVLPYVMIAGHTTSACGINTVGLRRRGFSSAAIDCLRRAYKIIFR
                     KGLTVQQAVSELELIQNECPEIIPMIDALNQSTRGIVR"
     gene            42955..43359
                     /gene="crcB"
                     /locus_tag="VAH76_000043"
     CDS             42955..43359
                     /gene="crcB"
                     /locus_tag="VAH76_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEO1384707.1"
                     /translation="MVVAPYLAVAIGGSLGAMSRYLVTIMAQNAWGIKFPYGTLLVNT
                     LGSFLAGFFLIVLVGRFSAEESFRLFLFTGFLGAFTTFSSFAAESLFMFEQGYWFKLI
                     TNILVNNVGSLSMVFIGTLVAKYVLLGHQGSN"
     gene            43381..44661
                     /gene="serS"
                     /locus_tag="VAH76_000044"
     CDS             43381..44661
                     /gene="serS"
                     /locus_tag="VAH76_000044"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO1384708.1"
                     /translation="MLDNQLLRENPQYVATQLLKRGFQFDAVTFSQLEEKRKALQVST
                     QSLQNERNLRSKAIGEAKSRGENIEPMREEVNKLGAKLEQQKTELDEVLKQIEVISLS
                     LPNIPHESVPVGKDELDNQEIRKWGDVPAFSFPVKSHDELGEALGQMDFALAAKITGS
                     RFVVMKGHLARLHRALIQFMLDIHIQQHGYQEIYVPYIVNADSLLGTGQLPKFEADLF
                     KLTGDNGYYLTSTSEIPVTNTVREMILSAEQLPIRYVCHSPCFRSEAGSYGKDTKGMI
                     RQHQFEKVELVWITKPEDSYNALEQLTQHAEVILQRLNLPYRVVALCTGDIGAGSAKT
                     YDLEVWLPSQNTYREISSCSNMEAFQARRMKARFRNPDTNEIQLVHTLNGSGLAVGRT
                     LVAIMENYQDEHGNIHIPDALKPYLGGIDIISVK"
     gene            44920..45939
                     /locus_tag="VAH76_000045"
     CDS             44920..45939
                     /locus_tag="VAH76_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1384709.1"
                     /translation="MNEVIIREITSEDKEMFLCAMQSSHALHHPWVKAPITPEEFDEY
                     FSRYQKPNPKSYLLLSDNNLAGVFNISEIVRGYFQNAYLGFYAVAAYAGKGYMSTGLK
                     LILTKVFKDIGLHRLEANIQPENTRSIWLVKKNGFRYEGFSPRYLRVNGVWQGHEHWA
                     MTYEDFIKDNNEALEKDHINIVAYNTEWPLLAKAEMVKLRASFPANSVIDIQHVGSTA
                     IPGMASKPIIDIQIAVRSLEEMKIIAVPILQKLAYEYWEDNPDPERMFFVKGMPPYGN
                     GRTHHVHIVETSSRHWKGKIFFRDYLLSHPEAAKKYQQLKIKLAQEHKYDREQYTDAK
                     SEFIN"
     gene            46134..46397
                     /locus_tag="VAH76_000046"
     CDS             46134..46397
                     /locus_tag="VAH76_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384710.1"
                     /translation="MADQPPKSKWHEIKRLSEGDNKVDNNQAPVSTADRIKKLYLDTQ
                     VGSPIDTVEEADAQRNNLGPKDTKIENSSPENDEPNPDIPTPG"
     gene            46737..47669
                     /gene="legD2"
                     /locus_tag="VAH76_000047"
     CDS             46737..47669
                     /gene="legD2"
                     /locus_tag="VAH76_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegD2"
                     /protein_id="HEO1384711.1"
                     /translation="MDNLLNEKKLTIFTSNEIQDNLNRVLKNYEQNGIIGLNGYLNQA
                     NRISLLNELEIIQKDAEQDIVRYWNNKIIHFYSKDPLKPVSVDREYVTEPYFQASSNK
                     AHVFYEVIDNMRVVNRIGHGMHLINRYPMMQRAVYDSPVLLDLLKGIGFKKPICHLSV
                     YIPKYPNGIGSEVKPHQESTFAFTEPQSVVVLWVALEDALIENACMYGVLGSNHWPLK
                     WVSKVDRETKTRHFEQVHQLHIPDFMTEREFYTALEVKAGDALLFHGNFVHCSPMNTS
                     KNSRKALSFQFIETYEVDYPETNWLYPPNKVYLY"
     gene            47705..48517
                     /locus_tag="VAH76_000048"
     CDS             47705..48517
                     /locus_tag="VAH76_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1384712.1"
                     /translation="MSMYDKRQFFKTYFSSASEKSILVKLLDKRFHPPFHTANILDLG
                     CHDGALMKNIVNAYAKRLPEKLVITGVDPSLQAIKEYAKADFPIPVQTNTFAGTAEDY
                     FDVCTDHFDWIFASQCLYWSPDLPKIIKQIHEAGDSALIVIRGCHGIYEIQSQFRHYI
                     GNHLEKFYTDKEVEMALRTQKILFQKESLSTFIRLPDKDSIELKWLILFFLQMEEESL
                     GDKIFCEVRDWITSRASGSIHHEVHFFWLGNAIFTGDYNRNFPVKSKKNSRG"
     gene            complement(48507..49337)
                     /locus_tag="VAH76_000049"
     CDS             complement(48507..49337)
                     /locus_tag="VAH76_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO1384713.1"
                     /translation="MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYY
                     EEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDHCKPYDDTASLTIQVQQSFASSL
                     EHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
                     ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLS
                     KKYEKSIEQIIFRFSQQIGMLPLTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
                     "
     gene            complement(49776..50624)
                     /locus_tag="VAH76_000050"
     CDS             complement(49776..50624)
                     /locus_tag="VAH76_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1384714.1"
                     /translation="MTDLRTEIESLIHEGLLPDREYVSAKRFINHYATTSKFWDGLAL
                     VGITPIINGLYAGYYASRAVWATLRTVGNLLILKPGLAFDAIKDVGCHLTLSTCLLLM
                     APIHALTHAVELLTRVISSWFVGQEQRYDLLETGLMAKWANRANGYQMVNELPSSNYF
                     NKARFFSSYNNAWDCLEQMASPITSLAKSGFHSLVEALYGVDSAIECIVNLAICKPLH
                     AKENFYNLQVHFGLALSLAVMAPINALVEGIAFMTRLGTTWVSACLKPEVTETEQENT
                     TAYQFN"
     gene            complement(50961..51428)
                     /locus_tag="VAH76_000051"
     CDS             complement(50961..51428)
                     /locus_tag="VAH76_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384715.1"
                     /translation="MNAENNVLNTLNSLSKEINKLYGYVTIAGDNFGEPAINSGPCGP
                     FANAFYKIWNQKFTEKVNIAFIMMKNSDECWHILIRLPNGLLFDGGFGVHNEDKWNKD
                     TFDIEDMLEYDLQLLEKHSGGLNRTYPRCCPDFSIDVITNLIHKHLELVEKNK"
     gene            complement(51412..52170)
                     /locus_tag="VAH76_000052"
     CDS             complement(51412..52170)
                     /locus_tag="VAH76_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1384716.1"
                     /translation="MTKLKNLDTYLSLCTEVYDLSKPNPPEEDYAFYRSYVINVKGPI
                     LEPMCGTGRFLLPLLEEGFEIHGFDASEYMLEALNIKAKAKNLKPTVWRGFVENLTRP
                     EKYNLIFIPSGSFCLLIDPATVRLALQTFYNHLSDEGILLFEGETLKAVPQLDVWRGS
                     VWHKPNGQMIMLSQLATLKDQVCHSIGKYELVHDNSIIHTEVEELRVRIYDPQELTAI
                     LKSCGFKNVRLIKAFDSAATPDAQDETIVYECRK"
     gene            complement(52136..52744)
                     /locus_tag="VAH76_000053"
     CDS             complement(52136..52744)
                     /locus_tag="VAH76_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO1384717.1"
                     /translation="MTPHIIGISGITGAGKSTLAKALSLDLKATLISWDDFDDISLEP
                     EDYIEWYHKGCNYSEFQREDLAKVLAELKAKREIMHPVLRTLLNPAEYIIFDAPLGKL
                     HVQTGEYIDTCVHIEVPLDISLCRRILRDFKDKLQTKENLLEEISFYLNHSRPLFFDD
                     ELKQNATIVIDGLLSTEVQIQIVKNFLIGKQDDKIKKPGYLS"
     gene            complement(52741..53208)
                     /locus_tag="VAH76_000054"
     CDS             complement(52741..53208)
                     /locus_tag="VAH76_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1384718.1"
                     /translation="MITIDLLKNHPQSIARCATIWHEILGKIWVPEIPVEQVIQRFSE
                     HLNADKLPITFIALDTGKPVGMCSLRENDGIRPEISPWLGSLVVDPAYQRQGIGNRLI
                     EATKNKARDLGFNKLHLFAFDPTIPNYYSRLGWAIIGMDKFKGHPVTVMEILL"
     gene            complement(53360..53923)
                     /locus_tag="VAH76_000055"
     CDS             complement(53360..53923)
                     /locus_tag="VAH76_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1384719.1"
                     /translation="MNINFEKANASHIELIFSWLKEPFVQEFWDNTQAHKDDILNFIN
                     GRTEPSSYADGKYVYWIASGNGHPFAMLMTIQETSEDEIDEIKLINLSKTGHSYGIDY
                     MIGDKNYFGIGYGARTLIEFIDFFIKEVDPKADTFLIDPASDNPRAKHVYIKAGFEYV
                     ADFLMSGDVSGAGKLHHLLIKRIGLIK"
     gene            54425..55159
                     /locus_tag="VAH76_000056"
     CDS             54425..55159
                     /locus_tag="VAH76_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO1384720.1"
                     /translation="MKEYISALSIDRLTSYEILCPSKVDTDIIGAYHWNLLISQAFYP
                     FIHSVEVALRNSIHHAATKKFENEFWFDIVVTDGKSRSILEDTKNDLHRRFGQVTASD
                     IVAGLTFGFWTNLIKQKTYTDQFNPNRLWPDLIPSVFPHYARGHDDRKNISKRFEEIR
                     LIRNRLFHHEPIWKFKNAQTPQECIAELRRKFNDTFKAIGWISKYKRNCLREFGFVES
                     FKNNCTLETLESYKVKGAEILQQLAD"
     gene            55162..56385
                     /locus_tag="VAH76_000057"
     CDS             55162..56385
                     /locus_tag="VAH76_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO1384721.1"
                     /translation="MKAKINNTLIKKLLPRDKEYEVHDLDLKGFMIRVFPSGTMRYVC
                     QYKRGKKINLGTVGVMTAAQAREKAIEVLSNANKGIEPTAQKGINKPKTLQEFIESDY
                     KPWVLSTHKRGQETLAGLIRCYKAFFSKPLGEITPALVEQWRVKRLNEGTSQATLNRN
                     ISMLKSLMTKAVEWGFLKENALGKLKPYKIDRSPKIRYLSFAEEARLREALIERDNQL
                     RLMRKNGNLWREKRGYDLLPELSEDNACDYLMPMVLLSINTGLRRGELFHLTWDMVNL
                     SEGSLILSGEITKNKHSRYIPLNDEAYKILDNLHKKAERKEGLVFPSKNNQPFNHVKR
                     SWGSLLKKANITQFRWHDLRHHFASKLVMAGVDLNTVRELLGHSDIAMTLRYAHLAPE
                     HKVNAVRKIDWSMQG"
     gene            56390..56809
                     /locus_tag="VAH76_000058"
     CDS             56390..56809
                     /locus_tag="VAH76_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384722.1"
                     /translation="MNSHTSKYAERNRPIRRIERFILKIMIFVVIPISCILGIVYPTS
                     KISNSLPVLFSLAGLIQLEVAGLFEYIDYLGNELEKIYDKSGRVPSNITRAIYEYYNP
                     EPTVKAFINNQLYLNKRVGFYLLLIGGLIQLFTIWLE"
     gene            complement(56911..57585)
                     /locus_tag="VAH76_000059"
     CDS             complement(56911..57585)
                     /locus_tag="VAH76_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO1384723.1"
                     /translation="MKNATKVKKDSSAMGQRIKRARMLAGLSRKDLEEKHGISIHTLQ
                     SWELGRNPLNKAKAASLVGILNQYDVSCSIDWLLEGIGKGPSVIENEFQNYPFLADTV
                     GNLLASEQAIQKEIDFFKTNNPNAVVITVSDDAMEPEYKVGDIVGGIKFINQNKKNQC
                     VGHNCIVETTEGTFFRRLIKSEDKYLLVCNNNRTSVSDPVMSADSILSIAPVIWHRWK
                     FDALSN"
     gene            57770..58651
                     /locus_tag="VAH76_000060"
     CDS             57770..58651
                     /locus_tag="VAH76_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384724.1"
                     /translation="MDMLINQDELAAMSGLPHIQQLAYLRGIRPYMDVKTGITGIKRR
                     ISYQSISEQLYIEPHQGIKSQSFSRDQVRRAVSGLVRAGMIEVQSDGMQLILKCPLAS
                     RHYSAQNKAAINPPQKATIKPHVETLVNNEFSEGEAIKADIAEPQKAAIPLKDNNYIY
                     LLSRFEQFWNLYPEKKSHERAFEAFRKISPDEHLLRMMLQALENQIKARNVKIAHGEW
                     VPPWKYPSNWLVQKCWEDEVKIEVTQEKRNAKRHPNTELTTVDPFWNPEGEDTASTSE
                     DEYEQSNVINLRCYRQQ"
     gene            58668..58976
                     /locus_tag="VAH76_000061"
     CDS             58668..58976
                     /locus_tag="VAH76_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HEO1384725.1"
                     /translation="MFTRFAVFYGHLWRSQFKSDGFLEFAKKEWMAGLSKFSDDVLNQ
                     AIVECRDFCEMPPSLPQLIRICRDIKKRNNVYVTPEEVAPASAEVAEANIKQCKAFLF
                     "
     gene            58997..59263
                     /gene="csrA"
                     /locus_tag="VAH76_000062"
     CDS             58997..59263
                     /gene="csrA"
                     /locus_tag="VAH76_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEO1384726.1"
                     /translation="MLVLTRRVGESVVISEDIYCTIVGYQNDEVRLAFDAPKSIPIHR
                     DEIQRRIYRDQIKDNKFVDKAANNESIVDRLINKFKSSASPTNS"
     gene            59274..60239
                     /gene="trbB"
                     /locus_tag="VAH76_000063"
     CDS             59274..60239
                     /gene="trbB"
                     /locus_tag="VAH76_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HEO1384727.1"
                     /translation="MTIDEEQLITVKDRAKEKLRRDLGGLIERALNDPKTVEIMLNAD
                     GRLWQERLGETMRCIGSINEVRAESIIKTVAGFHGKEVTRFKPMLEGELPLDGSRFAG
                     QLPPVVSRPTFAIRKKALSVFTLDEYVRAGIMTDEQNEIIQQAVSNHRNILVIGGTGS
                     GKTTLVNAIIHEMVKSAPHERIFIIEDTGEIQCSAENCVQYHTTMDVNMTQLIKTTLR
                     MRPDRILVGEVRGSEALDLLDAWNTGHEGGAATLHANNCLAGLHRLKSLITRNPAAPA
                     EIEPLIGEAVHCVVHIARTPDGRFVKEIISVEGFKNGQYNIKKLV"
     gene            60251..60628
                     /locus_tag="VAH76_000064"
     CDS             60251..60628
                     /locus_tag="VAH76_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEO1384728.1"
                     /translation="MNQLSSFNHKKIWMMGAVILFLFLMTHPASASTAGGGLPFDSWL
                     TKIQKSITGPFAFSAAIIGLVAAGATLIFGGELNGFMRTLVFFVLVLSFLVAAQNTMT
                     AITGKGAEISKPSVEMTAIESQP"
     gene            60625..60924
                     /locus_tag="VAH76_000065"
     CDS             60625..60924
                     /locus_tag="VAH76_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HEO1384729.1"
                     /translation="MSLRTISIRRCGNRPSLFMGGDRELVMFSGLLSAILVFAAQDWL
                     AAIAGIVMWFLSLKGLRLMAKSDPYMRAVYLRQRRYQAYYPARSTPFRENKRGYQ"
     gene            60921..63446
                     /locus_tag="VAH76_000066"
     CDS             60921..63446
                     /locus_tag="VAH76_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869966.1"
                     /note="type IV secretion system ATPase VirB4 family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HEO1384730.1"
                     /translation="MIELIAFLISTTGLVILGTLFWEIRSKSCGHHVKKHRSHTAGLV
                     DLLNYAAVVDDGVIVGKNGSFMAAWLYHGEDNANTTDEAREMVSFRINQALSAMGSGW
                     MIHVDAMRRTAPGYSARGYSHFPDPISMAVDEERRQLFEQLGTLYEGYFVITLTWYPP
                     VLVQKRFVELMFDDSGERLSQKAKTHQLIETFKQSCRNFESRMSSAVHLERLGSDDKG
                     DTVMQDNFLRHLQYCVTGINHPVNLPKNPIYLDALIGGQELTPGITPKIGRKFVQCVA
                     IEGFPMESYPGILTALSQLPIEYRWNSRFIFMDAHEAVAHFTKFRKKWKQKVRGFFDQ
                     IFNTNSGIVDEDALSMVNDAQSAIAETNSGMVGQGYYTSVVVLMDEDRAQVEKAALFI
                     EKNINALGFAARVETINTMDAFMGSLPGHGVENIRRPLINTMNLADLLPTSSIWTGEN
                     KAPCPLFPPLSPPLMHCVTSGNAPFRLNLHVRDLGHGIMFGPTRSGKSTHLGLLALSW
                     RRYKDARIYSFDKGLSMYPTCKATGGEHYTIADKDSKLAFAPLQFLESKADRAWAMEW
                     IDTILALNGLDTTPAQRNEIGYAIISMHQSGSKTLSEFVMTIQDEHIRETLKQYTIDG
                     LMGHLLDAECDGLGLSSFMTFEIEHLMGLGEKFALPVLLYLFRRIETSLDGRPTLILL
                     DEAWLMLAHPVFKNKIAEWLDSMAKKNCAVFMATQHLSHAAKSGILDIIVESTATKIF
                     LPNLYARDPETCVIYERMGLNPRQIDIIAQAQPKRDYYYVSEKGQRLYQLALGSMALA
                     FVGATDPDSIARMKQLELKYSDGWVSQWLTEKGIQLEQYGEAA"
     gene            63443..64186
                     /locus_tag="VAH76_000067"
     CDS             63443..64186
                     /locus_tag="VAH76_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HEO1384731.1"
                     /translation="MKKIKQWLNTKRPEKKNEALTENPFLNARRAWNVHTAGLMKSVQ
                     VWQLVGLSSLLLALAAVGGLISIGSQSKFIPLVFQQDANGNTLSVTRADRVGQANIDD
                     YRAAAAHFIENIRMVSSDVELQKKAVFQVYSYLNQNDAALAKVQEFYSNKQQSNPFER
                     ATHEIVSIEIRSVLQESDETWQVDWVETVRNRDGTLKEKPYVMKAMITMYQDNELNDV
                     SSESILKNPHLIFIRDFNWSYELKHGEQR"
     gene            64183..65058
                     /gene="trbG"
                     /locus_tag="VAH76_000068"
     CDS             64183..65058
                     /gene="trbG"
                     /locus_tag="VAH76_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEO1384732.1"
                     /translation="MKKIMLTFCLLMPLTGFALDNNELATRYFSENDPKLSNQEKQAL
                     DIAERWQKGDKTSKPFPSSDGSVSFVYGSGQIRVVCAPLQVCDIALQAGEQFNDMNVG
                     DPRFIVEPSITGSGAAQQIHLLIKPKDVGLDSSLVVTTDRRTYHFRLKSDRTEFMPYV
                     SFIYPDEAKAKWQFIQNVQAERRKMDTLPETNEYLGNLNFNYRIQGHARFKPLRVYNN
                     GVKTIIEMPKTMAQSEAPVLLVLRSRGLFQKSESVMVNYRLQGCRYIVDSVFDRAILV
                     IGSGSSQEKITITRC"
     gene            65061..65471
                     /locus_tag="VAH76_000069"
     CDS             65061..65471
                     /locus_tag="VAH76_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HEO1384733.1"
                     /translation="MKELSVLLFALLLSSCASQRYGNFTQVSQGRDVYLAQDAASQLT
                     RVYPPAQNTFCISQKVRDAFGISLIHEMRKKGYGVIENNCPKQKANFFYVVDEIKATN
                     LYRVSLFIGSQTFSRIYAKTGGKIPPVSAWTHKE"
     gene            65477..66727
                     /locus_tag="VAH76_000070"
     CDS             65477..66727
                     /locus_tag="VAH76_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEO1384734.1"
                     /translation="MNQNNDFLSKDYSPQKLQTAGVKRVNNVPLIIVIGILTVFVVLI
                     AFVANKRANAQNQSPEIVKIKSQKKNTMSLAYEVVGNHKVPVVAPQTETITIKQEASL
                     PILEQQDLPQKQETLDQNVTDAEIERIRQEKTQEFEEAVKAKTSIMVDNVRLNNRASV
                     RTSMSGNDVTFNVDPSTAFKEQLMLVQGMHNKPMPQTLGGEENEMRWHLNSRLESPNS
                     RYELRAGGVIPGVMVSGISSELPGQIIGQVSQNVYDTATGKYLLIPQGTKVFGIYSSD
                     VSFGQNSVLVAWQRLVFPDGKALDIGSMPGADSAGFAGFRDQVDHHYARIYGSALLMS
                     GIVAGITYSQNSNQPNQFGYSQPTAGSVLSQALGQQLGEVTSQLVSKNLNVSPTINVR
                     PGYRFNIIVVKDLTFKQLYRQFAY"
     gene            66743..67483
                     /gene="trbJ"
                     /locus_tag="VAH76_000071"
     CDS             66743..67483
                     /gene="trbJ"
                     /locus_tag="VAH76_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HEO1384735.1"
                     /translation="MKFRIMLIYLWTLHVFASGAPVFDFANWIENGKIILNQVSQYKT
                     QIDQYNNQLQQYQNMLENTKSLTSFQWDNANSVINNLLESTNTIDYYKQEAGSLQGYL
                     DRFQSQEYYQKTPCFNGNGHCSPEELRKIKQSRLSTSVAEKRANDAMLKGIDKQQQSL
                     KDDSEKLRVLQSQAQSAVGQKQALQAASQLASNQSHQLLQIRGLLLAQQNAQAVKDAA
                     NANKEAIQAAGDEQFRAGTYHKSSGKKW"
     gene            67510..67722
                     /locus_tag="VAH76_000072"
     CDS             67510..67722
                     /locus_tag="VAH76_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384736.1"
                     /translation="MKRLSLSIMVFACLLSGCESTETKARKKSEHLASLTCDSPREGH
                     TKEELQAIVEACFRGGSYSKSSGKKW"
     gene            67727..69124
                     /gene="trbL"
                     /locus_tag="VAH76_000073"
     CDS             67727..69124
                     /gene="trbL"
                     /locus_tag="VAH76_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEO1384737.1"
                     /translation="MNKKTTTSLLILCLLGFSVCCHAQGHGIDSRDLLDNILYRFSST
                     ASMWSQTILSYARYLFWSLATISMVWTYGLMALRKADIQEFLAETVKFLVVVGFFYWI
                     LDNGPAIATAIMDSMRLLAAKASGINEHVSPSDIVDVGFDIVSKAIDSSSIWSPAATT
                     VGLIVAGLILIILALVSINMLIILITAWILTYGGIILLGFGGGRWTQDIAINYYKTVL
                     GIALQAFAMILIIGIGKSFVDQYYAAMSENIMLKEMFVMMVVAVLLLVLINKIPPVLS
                     GIVSGGGSGGGGGLGLGGALGAAGIAGTALAGAAGAAAVQSAGGLSALQAAFKAASHS
                     IGASGAGSVSGDSPGSKQGSLAQAMGQASKFAGSFGSHLASGAFDVAREKAASMKDAF
                     SAKAADTTGGKIADAIHQRISSNAESPTINQQVSESQNVTSMGAPEGSFSSCNINSDD
                     EISQFVNQKASGDGQ"
     gene            69121..71010
                     /locus_tag="VAH76_000074"
     CDS             69121..71010
                     /locus_tag="VAH76_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO1384738.1"
                     /translation="MSLNKPNKAIGPQVRQKHNEKTNKQLVILVALSLFISMQVGTQF
                     VAYKLHFSDELGFSLNHVYFPWQSLWWSLKYHHYYPDYFNAAFGLMAMSGSLFLMLIV
                     FVAKHLKKENISEYLHGSARWANRDDLKNAGLFGNEEGVYVGAIEDEKGDIHYLRHNG
                     PEHILTYAPTRSGKGVGLVIPTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRF
                     EPATLKGSARWNPLDEIRAGTEYEVGDVQNLATLVIDPDGKGLETHWQKTSQALLVGF
                     ILHAIYKLKNQGEPATFPNIDRMLVDPNTNIADLLIEMTQYPHVDGKTHFVISASARD
                     MIDRPEEEAGSVLSTLKSYLALYRDPIVAYNVSASDFCIKDLMHHASPVSLYIVTQPN
                     DKARLQPLVRVMLNMVVRLLADKMEFERVDDGRGNYFVRTKKTYKHRLLCMIDEFPSL
                     GKLDILQESLAFVAGYGLKFYLICQDINQLKSRERGYGPDETITSNCHIQNAYPPNRI
                     ETAEHLSKLTGQTTIVKEHTTTSGKRFSTFLNQISKTKQEVSRPLLTVDECQRMPGPK
                     KDENGLIKEAGDMVVYVAGFPAIYGKQPLYFKDPVFIARASVEAPEQSDILRARLRED
                     EEIRL"
     gene            71007..71552
                     /gene="traF"
                     /locus_tag="VAH76_000075"
     CDS             71007..71552
                     /gene="traF"
                     /locus_tag="VAH76_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HEO1384739.1"
                     /translation="MKKFTSWIAVLMLSIIGMAFLFMLMGFRVNTTDSIPFGLYRITN
                     IKNIKNAYVIFCPDDRPVFKQGLDRGYIGSGLCPGGYGYLMKKVVATNGDQISITSDG
                     VFVNRQLILFSKPTLIDGRKRPLPQWRTIDYQIKEDELITMTSQSECSFDSRYYGPIR
                     IGQIKGVIKPIWVNATSGEIA"
     gene            71549..71713
                     /locus_tag="VAH76_000076"
     CDS             71549..71713
                     /locus_tag="VAH76_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384740.1"
                     /translation="MNKEAELMDVISEKLDDLMVPGFIAEVTPIEAEIMGAFSEDALS
                     EDDAKEAAYD"
     gene            71706..73892
                     /locus_tag="VAH76_000077"
     CDS             71706..73892
                     /locus_tag="VAH76_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HEO1384741.1"
                     /translation="MTKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVT
                     GKRYRGINALYLMLNQSGDNRWLTYKQAQSIDAQVRKGEKGTTVQYWKFTDEKIKNDD
                     AGNPVLDEQGNPVKVQVNLERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLL
                     LNSGAAIFHSEADRAFYRVSTDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDR
                     DLRHPFGSEAYAKEELRAEIASMLLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFR
                     ASADAEKIVNHICSLEQAQDIQHEQSIVNRDEKNNEETLMESEKNIPEKVWLSIPFKQ
                     KELAKSVVGKLPDGTPGIAWDKTQKCWYAKPGVSIEKIKAWLPENQNSPEDKALSPAD
                     EFKEALISLGAVLTGEHPIMDGKTHRIQMEDDKTGEKAGFYVAHLDGIPAGYIKNNRT
                     GAELKWKSKGYVLTDEQKAALKAQVLENQKNRELELVERHKSTALRLKQRLSKMSEIT
                     EPTPYMKSKGIQVHPDVYTDDDKKLTCIPATDIEGTIWTVQYIAEDGTKRFAKDSKKE
                     GCFHVLGGMNKLFDAPVIVIAEGYATAATIKEACGLPAVVSAFDSGNLKSVAKVLHEK
                     YPYTPVMIASDDDKHLELTQGINPGKEKALEAAGAVNGFIVMPTFAPHEQSLNPKKFS
                     DFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQKSNGIHIA"
     gene            complement(73894..74352)
                     /locus_tag="VAH76_000078"
     CDS             complement(73894..74352)
                     /locus_tag="VAH76_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO1384742.1"
                     /translation="MTVQLIKNFDINIDRYPNQYVGCLVLTKDNKILLQQRPHDWPTY
                     PDYLCEFGGRIEHNESPEQAVIRDLEEELGGDVKLKELVSFGAITESMSKHKELIHTF
                     FWHDKEGTITGCYEGLPRFFNESAHILLQTKITDGLRWLIDESKKLHLIK"
     gene            complement(74363..75130)
                     /locus_tag="VAH76_000079"
     CDS             complement(74363..75130)
                     /locus_tag="VAH76_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384743.1"
                     /translation="MDDKKLILDAANRYGFNLEFRTKKVLEEKNFSVLMNQLMKSGDE
                     FVEIDIRAAQYTGREWLIECKGSSDSSHLILIKEDSSNDPKSYNTKRHAIQDSNYRIA
                     QFKPDENQYFFTFTGDFFNKTGQQLKKISKNDSENNFFKAQYQILSAIKAISLTDTDK
                     DKSKDFPIIIPMIVTNAKIWVIDYNKSSEPGVSQHKWVLHKVKIKNNLFIVPKYEIEY
                     DTISILVLNIDYLDEFLGCYSYNINGEITIGNSELAK"
     gene            complement(75238..77103)
                     /locus_tag="VAH76_000080"
     CDS             complement(75238..77103)
                     /locus_tag="VAH76_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO1384744.1"
                     /translation="MIIRHIPMKSARLSSFSALVKYITDEQNKQERVGKVRISNCNSV
                     DPTWAIHEVLATQARNQRAKSDKTYHMLISFAPGENPPNEVLKAIEERIISSIGFKDH
                     QRISAIHHDTDNLHIHVAINKIHPKAFNIIEPYRAYRTFSEVASKLEIEYGLEITNHQ
                     TRKSRSENLADDMEQHSGIESLINWIKRNCLEQINQANHWNEVHKILSLHGLEIHIKA
                     NGLVFYNEAGLMVKASSVSRCFSKKNLESRLGEFKPSPFSTERSRQNIYRYEPLNRKV
                     IGSEIYARYLYEKEHGKTLLAEKLKHLREAKARLITKAKKRGRTKRAVLKLMKAPRTQ
                     KKYLYQQISKTLLKEIEKVRQNYAKERKHLLELHKNKTWADWLQQKAREGDKEALTAM
                     RYHNRKNQSNYSLSAASSNFSLADIKQVDSITKEGTEIYKMDKAVIRNTGKEIKISKG
                     GSIATLKKAIEMAQQRYGNCIQVNGSPLFKKIILQITIQHNIPLTFADSEMEAQRQKM
                     VLEQERRYEQSRRYRFNDGGRTPRSNEAAGAAIGERGTLSSTKPNADSIRQGPPAKSQ
                     NSLRDLSQLDMVQLARRSKVLLPDNAHDQLERQRLKSDNYVRRKVSGLSRKVEHK"
     gene            complement(77100..77453)
                     /gene="traJ"
                     /locus_tag="VAH76_000081"
     CDS             complement(77100..77453)
                     /gene="traJ"
                     /locus_tag="VAH76_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HEO1384745.1"
                     /translation="MDDKNKSPSRKHGRHLRVPVLPDEEISIKSHAAQAGLSVAGYLR
                     RIGLGYQIHSAIDKDYILQLSKINADMGRLGGLFKLWLTQDRRVAHFDHRKVKALLDR
                     IQTMQAAMFEVVKKL"
     gene            77868..78194
                     /locus_tag="VAH76_000082"
     CDS             77868..78194
                     /locus_tag="VAH76_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HEO1384746.1"
                     /translation="MKKPLSERVIQNQSEKKNGRTNAKIQFIALKEDIREALDKGCSM
                     KAVWETLSDEGQISFGYKAFRHYVLKLIKSEQENIRDDKQSKSKTTNEIKGFTFNPIP
                     NPEELL"
     gene            78194..78919
                     /locus_tag="VAH76_000083"
     CDS             78194..78919
                     /locus_tag="VAH76_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HEO1384747.1"
                     /translation="MAKIHITMQGKGGVGKSFVSATTAQYKLNKAQSPLCIDTDPINA
                     TFHGFKSLNVERLNIMEGDEINPRHFDTLIEKIANTQNDVIIDNGASSFVPLSHYLIA
                     NQVPTLLKDMGHELIIHTVITGGQALLDTINGFAQLVNQFPEDIRYVVWLNPYWGKIE
                     SEGKPFEQLRVYKETKDRIAAIINIPDLKEETFGHDLSNMLQQKITFNEAIDSPDRNI
                     MTRQRLKLIRDQLFSQIDNATVI"
     gene            78919..79368
                     /locus_tag="VAH76_000084"
     CDS             78919..79368
                     /locus_tag="VAH76_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HEO1384748.1"
                     /translation="MSDKINEAIQYIAGKHGVVLGRDDPVLILQTMNDRLLEENRKAQ
                     QEMLAQLKEEMENISSQWKVDAKEKAEKVLNAALASSKEAMNKILREATNESVHVMKK
                     LISDSLTEANDLTQQTRKANRFTLLASASMMTVSCAFMLFLLINFAR"
     gene            79394..>79741
                     /locus_tag="VAH76_000085"
     CDS             79394..>79741
                     /locus_tag="VAH76_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEO1384749.1"
                     /translation="MKNENNKKIQNLSTFSWSIAEILRGDFKQSEYGKVILPFVVLRR
                     LDCILEPSKDAVISAYENLPEGIDDHTKDMMLFSAVGGGLKVYNYNTLTFSKIRNQDP
                     GDVHKNLLDYITSF"
BASE COUNT        23960 a        14462 c        16645 g        24674 t
ORIGIN      
        1 gctaaaaaat taactgaaaa attatagggg aatataacaa acctgggcac tccaggaaga
       61 gttgctgtat gagaatttag tttaaggagt tagaatcaat gcacccaaat tattatttgt
      121 cacctttagc tgtagctata gcattaggga ttgcttcacc tgtgaaggca gccgacccga
      181 tccctttaca aaagtcgtct ttttctgaag tcacacaaaa atttcagttg actttgcctg
      241 gtgttatgaa aggtgctgta gtttcaacga atagtttgca gtttataaga cagcatactg
      301 atgggaataa agtgacgcat gttcgtatgc agcaacaata tgccgggttt ccagtttttg
      361 gtggttacgc tatcctacac agtaagaatg ccactcctag tttagcgacg gcaaaatctg
      421 atgtgaaaat gaatggtgtg atttatgatg ggttgcaagc agaattagga cagcctaaac
      481 cttcatttgt gaaaaatgct tctatggcat tacaacaatt taaggataaa tacgcgaata
      541 aacaagttag tgaagatcaa gtaactccaa tgatttatat tgatgaaaaa catcaagcac
      601 attgggctta caaagtcagc gttttagtta tccatgacga tagaattcct gagcggccaa
      661 cagcaattat tgatgcagaa accaataagc cttttgttca atgggatgat gtaaaaacgg
      721 aaaaagttca agcaaaaggg atgggctttg gtggaaatcg taaaattggt gaatatcaat
      781 ttggcaagga cttgccatta ttagaaatca cacgtgattc aagtgttgaa atgtgcttta
      841 tggaaaatac cgatgttaaa gtagtcgaca tggggcataa gtactattca aataacaaac
      901 ccatgcaatt tacttgtaaa gagactcccg atactcaatc tactaaaact tattataccg
      961 gatattcggc tgatggttat gatagagata atggagccgc ttctcctacc aatgacgcat
     1021 tgtatgcagg ttatgtcatt aaacacatgt accatgattg gtatggggtg gaggcattga
     1081 ccaaatcaga tggatcgcca atgcaattag ttatgagagt gcattatggt caaggctatg
     1141 aaaacgccta ctgggacggc aaacaaatga cctttggtga tggtgacacc atgatgtatc
     1201 ctttggtttc tctaggtgta ggcggtcatg aagttagcca tggattcact gaacaacatt
     1261 ctggacttga atatttcggt cagtctggtg gcatgaatga gtcgttttca gatatggctg
     1321 ctcaggctgc tgaatattat tcagttggaa aaaacagctg gcaaatcggc ccggaaataa
     1381 tgaaagaaga cagtggttat gacgcattgc gttacatgga taaaccaagc cgtgatggta
     1441 tgtctattga tgtagctgat gattattatg gcgggttaga tgttcattac tccagtggag
     1501 tgtataacca tttattctac atattagcta atcaacctaa ttggaatctt cgtatggctt
     1561 ttgatgttat ggtgaaggcc aatatggatt attggacacc ttattcaaca tttgatgagg
     1621 gtggttgcgg tatgttgagt gctgccaaag atttgggtta caatttggat gatattaaga
     1681 agtcattgag tgaagtgact ataaattatc aatcttgtta tgtcgattaa ttcattttat
     1741 tgattgataa aattcagcca gaagtcgact gatgacttct ggcttttcca tatgaatatg
     1801 gtgtccacca tctaacttct caattttaat attttttact gcttgaatcc ttgctttcat
     1861 cttatccgaa tcaaatgaaa atcctttgct ggcccaaata aggtaagttt gagcttttat
     1921 ttctgtaagg cacgaaagaa cttgtgcttc agtcatttgt aatggagatg gggctaatag
     1981 cctgcgatcg tgtctccaat aatatagacc attttcttgt tgaattccgc gttcgcaaag
     2041 tgacttggca atatccagag aaacatatcc tttaacggag cgggcaagag cagcatgttc
     2101 aaacttattg tagccttttg ccttttttga ttgtttttgt gatagatagt ccaggtattt
     2161 tgacagttgc tggcatgctg tttctgccgg gtgagaaaaa gggcctaaac cttcaattaa
     2221 gcttaatgaa agaaaacgat ccggtgcaac ccctgctaca agacttccca agcaggctcc
     2281 catagagtgc cctagcaagt gaagcttatt tagtcccaag gcattaataa tttcaacaac
     2341 agtaaatatt ccatcagtaa aatgatatat gcatcctgga ggtaagtgtg aagaatgacc
     2401 atgtccaggc agatctacag ctataaaata gtaatcattc tgtaaatgtt cagcaatatc
     2461 atcaaaacta ttagcattat ctaaccaacc gtgcaaggca agaataggcg gattatcagg
     2521 atttccccat actttgcatg ctatagatag tcccgggata cttaaaatct ttgttttcat
     2581 caatttgctt tggtttgtag gtaatagtta tagcttatta ctttatacgc agggatgtaa
     2641 attcagtttt taaaatagtc aggacttgcg aaaaaaataa tcttttcgct aaaaaaatgc
     2701 ttttaatcca gtcatttagt ttatttaatc tccctcatta aaatcaattt ttgccttggt
     2761 cttggttttt ttcgcaagtc ttgatagtaa ttgaaataga caaaaagcaa taaagtaagt
     2821 cgattattgg gttgacttgt aacaaactat aaaagatcac aaaataaatg gctttactgg
     2881 tttttgggga agggccaata ttatgataaa taaaaataat tcagagtccg agcttatcat
     2941 gtttaatgac aaattctttt caaaatgtgg gttataatta tagagcaata cattaatcct
     3001 attaccatta tactgattat agaaacctaa atgagagata gagtatctat gcaacctgag
     3061 gtgcgcagcg tatcgctgat aacatataat attcataaag gttttggagt aggtgcagta
     3121 aggtttttgc ttccagaaat gcgtgatgct atcgctggat taaatccgga ctttgttttt
     3181 ttacaagaag tgcaaggtaa acatagaaaa cgcgaaaaaa gaattgtcag ttggcctgat
     3241 tcccctcaat gtgaatacat tgctgagaat atatggccac attacgttta tgcaaagaat
     3301 gcagtatatc aatctggcca ccacggaaat gctattttaa gcaaataccc gttcgagagt
     3361 tttgaaagta ttaatctttc aaacatgaac agagcatcaa gaggtatact tcatacccaa
     3421 ttaaaattgg ataataatat tattcatctt ttgtgtgttc atttaggcct attcaaggca
     3481 gagcgcactg agcaatgtaa ggcattgata caacgtatta aagatgttgt gcctaaaaat
     3541 gagcctttga taatggcagg agactttaat gattggcgtc atgttatttc taaaatcctt
     3601 gctgaaaact tagggattga agaagctttt gtcgcgctcg aagggcaaca tgcccgttca
     3661 tttcctgcaa taaaacctgc tttgtgtgtc gacagggtgt attttcgcgg gatgaaagtt
     3721 caagaggttg cttgtttaca gggcaaacct tggcggatgt tatctgatca tttacccttg
     3781 tatgcacgtt ttgaattatt atgacagggc tacctccagt ctttttgttg aaaaagattt
     3841 ttaatgcggt catttggttt attctaactc ccacatttaa aatttttttg ccttgacctt
     3901 ggggttttta gtaagccttg tatgattaag attaaagcga cagtcttcct ggtcgagaga
     3961 aggttctgtc gctattgcaa ctttactcca tgtcgttttg gtattcaccc aaacaagcca
     4021 tactgtttaa gcgggctctc ttaattaaag cctcttgcgc tttggcaata tttgctttgt
     4081 ttccacacca ttcctttaag caatcctctt gtaatgctct tccaaatgaa aaacttaata
     4141 accagggttg gtggccaatt tgattgattg cattcaagtt agcggtagcc tgttggggtg
     4201 tttggccacc ggagaggaaa ttaatggtgg gtactgacgc tggaacatta ttacgaaata
     4261 cactaatggt gtagtcagcc acttcttcag gatcgctaaa cggggtcact tctttaccac
     4321 aggtaaccat actcggttta agtataatat gctccaactc cacttgatgg ataaacaagg
     4381 tatgaaacag ctcatggaga accatttcac agacttcagc acaatgttct atgctatggc
     4441 tgccatccat taatacttca ggttcaacaa ttggaacgat gccgactgat tgacaagcag
     4501 cagcgtaacg gcccaaattt tcagctcctg ttttgattgc agttaggctg ggagtgtatt
     4561 cggaaattga ataaacgttt ctccattttg cgaattttgc acctaatttt ttaaaatgaa
     4621 gcaggcgctc ggttaaacca tctaatccct gggtaacttt ttcatgttct gtattggcta
     4681 aatcaacaag ccctttgtct actttaattc caggaataat gcctttatta gcaaaaagct
     4741 caggaattgg tgttccatgc tcatcagttt gagtaaaagt ttcctcaaat aaaatgacgc
     4801 cgttgatata ctgttctaaa tcaggagtgg tcgcaagtaa caagcgataa tctcttcgat
     4861 tctcctcggt attttctgta ttgattgacg caaagcgctt gccaattgta ctgttgcttt
     4921 catctgcagc taaaatacct tttccttcat gcagcatttg ctccatggta tttgataatt
     4981 cttcataact cataacgtgt ttatctcctc attatcgggt gtcgaccttt cgaagtggtt
     5041 tggcacctat taaatttaaa taacgactag tcatcttttg tcaaatccgg gttatttacc
     5101 gaaaggatga cactgattaa caatggtttt tgatgcggta tttcagcgtg aaatatattt
     5161 ttcacgtaca atatgttgca tgggaaaacc atgttctttt aaataattaa atgcctcgtc
     5221 gatcatacct ggatttccac ataaataaac catatcgcga gttggattta aattaagctc
     5281 tggaaatgca tgctgtacat aaccactgta ttcattgtct ttcaagtctt gtttttgcac
     5341 acggcttaaa tagggacgaa aacttgcttg aggatatttt tttgcaaagg cttgaaaatc
     5401 atctggatag agaatctctt cactgcgttg cactccttga aggatgacta cctgtaaatc
     5461 aggatgtttt tctattcttt gaccaagctc attaagcatg gctctataag gtgtggtacc
     5521 agtactagtc gctaccaata tatatcttcc tggagtttcg tctttaaaaa tcaaacgacc
     5581 gaaaggtcca ttaatgtgga tcacatcacc tggctttaaa ttgtagagta gttctgtgcc
     5641 tggtcctcct tcaaaatacc cggcagcaaa ttcaatgcga ttatcctttt tgggttcatt
     5701 ggcaatactg taactacgct ttaagttttt tccatcatgt tcaaaatgaa tagtgataaa
     5761 ttgtcctggt aaatattcaa aagggggtga taattcacaa ttgaatataa agtgctttac
     5821 cttgggagaa atcatgaaag actcttctag agtgatgggg aaagtattta tttgcataag
     5881 gaatatcaac ttttattcta aaatgattaa tataggggaa aaattatgca ttgcaagagc
     5941 taagcgcata taattaacat atgaacacga tagatttaat cactatatta ggtaatatta
     6001 gccaatccct ttatcctgtg caaaaactta tcacaggagg agcttatata ttgggtgtct
     6061 tatttttttt aacggcaatt ggcaagttaa aaaaaatcgg ggatcatcgg gctcaatcct
     6121 cctcacagga aaaaatgttt actccaatga tgtatttatt agttggggca cttttaatct
     6181 acttgccttc gggtatggat cttatggcaa ataccgcttt tggtgtcggt aatgtattaa
     6241 cttattccaa ttataatcct gccaatatct acagttcaat ggggttttta attcgcacgg
     6301 cgggtgttct gtggtttata cgtggatgtg tacttgtcgc tcattccagc cagccaggta
     6361 ctcaacaagg attaaaagga ttggtatttc ttattgcagg cgtttttgca atgaattttg
     6421 ataatacgat cgccatgatt aattctgctt tagattattt tgtcaaagca actttatcag
     6481 taaaagccgc agcgggattc tagtttttct gattgatttg tctgcgcttt aaagaaacag
     6541 gacttaggaa aaaccccaag atcgaggcaa aaatgttttt aaccaagaaa ctgggggttt
     6601 tcctaagtcc tgagaaaata aaactggagg gcaatccttt ggaatgttat aagtcttctt
     6661 caccacagat tttcaagttg ttagctttag caaaatccat aattgcctga taaacttgtg
     6721 ggcttgattc cttgagtttg ggatcaagta aaacacattt ataaccctca ctattaacgc
     6781 ttggttgcaa gagcggagaa taataaattc tactattttt aaaaacagct aaagaaccgc
     6841 tcatacgctc ggcccagtca cttgggcgaa atgttttgcc ttgtgaagta acaccctcaa
     6901 tgacaatagt tttatttttt cgattagaca taactctatg catattaaat acagtatgat
     6961 aattatatca tcatcatgct gggcctgcag ggaaaattaa ttattttatt gcaataaatg
     7021 gttgtttcgc tttagtcatt gccaattatt agtcacaatt gcatgctgtc tttatgacta
     7081 agcataagtt tccatagtag atttagttct acttatgata gcatgtcctt gatttaaaaa
     7141 tgaatgaggt atgattgcga atgaataaag aaaatcattc tgggatttat ttgttaccaa
     7201 atttattaac gactgtcagt ttatttgcgg cattttattc aatagtggct tccacaaagg
     7261 gacaatatga ggctgctatt gtcgctatgt ttataggaat gatcgctgac agtcttgatg
     7321 gacggatcgc tcggttaacc aatacacaaa ctgagtttgg tgctcaatac gatagtttgt
     7381 ctgatatggt tacttttgga gtagcgccat ccttgctgtt atataattta actctgcatc
     7441 aattaggaaa atttggttgg ttagtcgcat ttatttatac agcggctgtt gcgttgcgtc
     7501 tcgctcggtt taatactcag attggaaaat cagataaacg ttatttccag ggcctcccat
     7561 gtccaccggc tgcagcagta atggcttctt ttgcttggat ctgttttcaa aatgaatggg
     7621 caaatttgat tgtatcttta tttacagctg tcctggcttt gattgtttcc gtattgatgg
     7681 tgagtaattt acgctattac agttttaagg aagtagatta taagggcaag gttccatttt
     7741 tatatgtttt agtgatgatt cttttgtttg ttgcaattgc tgcaaatcct gcaatagtgc
     7801 tttttgcaag ttcaggtatt tatgctttat caggccctat gttgacggtg atttcattac
     7861 ataaaatgag gaaacaaaga agaaatatga gcaaatgaca tggattgtgt atttcttgat
     7921 gaattattat gtaaatctgt tacccagacc tgtgaacagt agatttcttc agattagtat
     7981 caattaaggt gtattgagac cttaggtcag tttattgaat cacaggttga tcataataaa
     8041 gatgttacaa gaactgcgag gaacattgat ggagaacagg aacctctgtg tgtcgcaaaa
     8101 agatatggag cagaggtaaa cagcgtagtt tagtattctt tattcacttt ccccaaatcg
     8161 attattgatt aattcgacta aagcttcatt catgcgttct tcatctggac cgtcaatttc
     8221 caacgtcaac tcactacctt tgttagcagc aagcatcatc acacccataa tgcttttgcc
     8281 attaacggtc tgagtatctt tggttacatc aatatggctt tgaaattttg ctgctgttga
     8341 gacaaatttc gctgaggcac gagcatgtaa accgagtctg ttgattatct ttattttggt
     8401 cttgatcata gcacgtacaa tgtatcatga ctctggcatc aggtacagtg attaaataag
     8461 tgattttagt aatcaaccct ctttctcagt gctatgactt catctaaaaa acggttattt
     8521 gttttatgtc agtttacata agcactgaga aggtgttgat attaaattaa agtaaaaggg
     8581 tttgtattta aaataacatt taatccctgt ctctatgggc taaaagtttt tctttatgtt
     8641 taatcagttg tcgatttaat ttgtctacca aagtatcaat agcagcatac atgtcttctg
     8701 attctgaact ggcatgcaac tcacctttgg taaccaatac ggtcgcttct gctatttgtc
     8761 ttactttttc gacatcaaat actacattaa ttgctctgat gtgatcaaaa tgacgctcaa
     8821 gcttatcaaa tttttcttca gtgaatgctc ttagtgcagg agtgacatcc ataccatgtc
     8881 cagtaatgtt gatttgcata aaacctcctt tcgttgaccg attaattaac tactaataac
     8941 ctttctttca ttggatgggg gaatccccat tgcttcgcga tatttggcaa cagtgcgtct
     9001 tgctacttta atgccttgct cacctattag ttgggcaatt ttactatcac tcaaaggttt
     9061 ttttctattc tctgctgaaa ttaatttttt tattaccgct cggattgcgg tggaagagca
     9121 ttctcctccg ctatcagtat tgacatggct ggagaagaag tactttaact caaaaacacc
     9181 tcgaggggtg ttaataaatt tttgagttgt aactcttgat accgttgatt catgcatatc
     9241 caaggcagaa gctacatcat tcaatattaa tggtttcatc gcttcttcac caaattccag
     9301 aaatccatgt tggtattcca tgatacaggt tgctactttt agaagtgttt cctgacggct
     9361 ttgaatgctt tttaaaaacc aacgtgcttc ttgtaaatta tgctttaaaa attgattatc
     9421 cagactattg tttgcgcgtt ggattaaagc ggcatattgg ctattgatac tcagcctggg
     9481 aagaatatct tgattcaaaa aaactttcca ttgattatct atctttttaa cagttaagtc
     9541 gggaataaca tattcaatat tatcttgatg gatcaaactt cctggttttg gatttaattt
     9601 atgaatgact tgtaatattt catgaattgt tttttcactt atttgatgat ttttcatgag
     9661 ttgtctgtag ttatgttgcc ctagcaactc aatgtcttca agaatgactt tcttggcagt
     9721 agccaaaagc ttgggttcga gtggaagttg ttccaattgc aaaacgagtg tttctgctaa
     9781 attaacagat gcgcatccta cggggtcaaa taattgaagc ctgtgcctca ctgcttctat
     9841 ttcttctata tccagaggaa aatcatcgct atttaagctg gcgtgaattt cagcaacggg
     9901 tatggttaaa aagccgtcat cgtttatcgc atcaataatt gttgtagcaa tgactcggtc
     9961 aatatcgctc ataggcgtca ggtcaagttg ccagttaaga tgatcttgta aattggtggt
    10021 agtgcagtgt aaattatcga aattgtaatc gttttcttca aaattattgc gcttgtttaa
    10081 gttattatac agctgtgacc attgaaaatc tgaatattca tcttggttct gattggtatt
    10141 tatttggacg tcttcttttt cttcaacagg agtggcttca agcaaaggat ttgattcaac
    10201 aacgagttga atttcttgtt gtaaatcaaa ggtggataat tgcagcagtc ttattgcttg
    10261 ttgcagttgt ggggttaacg tgagatgttg accaatacca atctgcaatg tcggtttcat
    10321 tcaaatcctc tttttttttt cactgtcttt aactatcagt ttaaacgatc cacgaggatt
    10381 atccagtgct tacgggcgtt aatttgaatt taaaagtaat ggaattacct atatttttaa
    10441 taaaatgata ggcttgtttt gaaatataac aaattggttt ttaaagtgga taatgagagg
    10501 aggtaagccc caaagacagg ggcctatcaa tttattttac ttttttctct ttgaacagta
    10561 cgtgctttcg tactttaggg tcatacatca ttaattccat cttttcagga tggttacgtg
    10621 gatttttagt agtggtttta tagtatccag ttcctgctgt ggattccatt ttcactttaa
    10681 ttgtaacagc tgccatggct tatccccttg ttatatcttc tcgcctttag ctctgatttt
    10741 atccagaact gcctttatgc ctaacttgtc aataattctc atacccttgg tacttaatcg
    10801 caaggtgaca aatcgatttt cttcttcaac ccagaattta tgattctgaa tgttaggcag
    10861 gaagcgtctt ttggttttgt tattagcgtg cgacacttta tgaccaacca tgggtcgctt
    10921 gccagttacc tgacatactc tagacattgt gttactccaa gataaaaaaa attttaacag
    10981 cccttaccaa tgcactacag tggtgtttat tttggcgggc tttcgttgta aaaaataacc
    11041 agatatgaaa tacaagagcg gttttataac aaaaaaaccc aaatattgca atatttttac
    11101 tttgttttca ttaaaaactg atttttcagg gctgttgtcc aaacaggtat ctggtaaaag
    11161 tagaatattt aatcaaaatc agtataatcc tgaaatttta tattgagtta ttgtaatgca
    11221 acgtaagatt aaaagttacg tgttgcgcgc tgggcgaatc agtaacagac agcaacaagg
    11281 attagatctt tggcttgaag actatgaatt aaaatttgat agtccctctc cctggaattt
    11341 tgcaaaggaa tttggccgtc atgatgcaga tactattgta gagattggtt ttggtatggg
    11401 tacctcgttg tttactatgg caatgaataa ccctcaatgt aattatctgg gcatagaagt
    11461 acataaagca ggtgttggta gtctggtagc ggatttgcat gagcaccaga taactaatgt
    11521 gcgggtagta gtgcatgatg cagtagatgt attacaaacc aaaataccag aaaactcact
    11581 tgccggggtt caaattttct ttcccgatcc gtggcataaa aagcgtcatc ataagcgacg
    11641 actgattcaa tccgaattta ttcaaatgtt agtaaaaaaa atcaggccat cgggatttat
    11701 tcactgtgct actgattggg aagattatgc agagcacatt ttaaatgttt tatcttcaga
    11761 atccgccttg tttaaccaac aaaaagaggg cggatactca ccacgcccgg actcaaggcc
    11821 gttaacaaaa tttgagcagc gtggtgagcg tctaggccat ggggtatggg atctggtttt
    11881 tataaaaaaa taaggaagtt acctatgata aagcatgcaa ttcttattag cggattgatt
    11941 gtttcctttt tagcttcagc agctgaaatg tcgtttctag cccaattgac ggaagtacct
    12001 ggtgcctctg ggtacgaaaa aaatgttcgt catttggtac aaaaacagtg gcaatcctac
    12061 atggcggagc taaagatcga tggtatgggt aatttgatcg gccagcgtaa agataatcag
    12121 aaaggaccga aattactatt catggcacat cttgatgaaa cggggtttat ggtggaatcc
    12181 ataacacctg atggcttttt gaaagttatc cctttaggcg ggatcgcaaa ctcggtcatc
    12241 tatgctcaac gctggacagt ttcaacaccc aaaggacctg tgttagctta ttctggcatg
    12301 gattctccgc atttattggg tgataagaaa atgtcagggt cccctgatat caatgccctg
    12361 ttcttagata tcggagcaga gaataaagaa caagctcaga aagaatttgc aatccaacct
    12421 ggccttgaag tcacaccagt aagtgagttt agccaattaa gcaagaacag atttttaggc
    12481 aaggccttgg atgatcgcct aggattagct gtcattagtg acgctctgag agcagtgaaa
    12541 caacaaccca atcaattata tcttgctgcc acagtgcaag aggaagttgg tatgcggggt
    12601 gccagcacag tatacaaagc gactcatcca gatattgcaa ttaatgttga ggtgggaatc
    12661 gcagatgatt atccaatgct gctggctgat agaaaaggtc gaattggttt ggggaaggga
    12721 ccatccttgt ttgtttatga caaatcaatg attcccaatc aggagttaat taactggata
    12781 atggaacttg ctaataaaaa taatatcccg ctacaacttg aggtggagcc aggatatggt
    12841 gaagatggct ccaaggttca ggcatcaggc atgggcgttc ctgtcgtcaa cattggtatc
    12901 cctattcgat atgcacacca acatgccggg atatttgata aaagagatta cgataataca
    12961 gttaaattgt tgaggttgat tgctgagaat ttgaatcagg aagtggttga caagattaaa
    13021 aagagttaac aatattttga tctcccattg tccattgtgt aatattggtg tcctataaat
    13081 agtttataaa taaaggaagt attattatgg attttgtaag tgaaatgaat aagattctac
    13141 aaatggaatt agagcaattt aaagaattta ttcaaaagaa gctggaagaa gataaaaatt
    13201 atttagaaaa gttattttgg ttgccgaatg gttctcaaat gactgtttta aattatttga
    13261 tcgaacaata ctctgagcct aagcttggta tagacgatgc aaatagggat ttgcttgcca
    13321 aaatagattt tgtccttcac gaggctaaag atgtgaatgt tggtgagcct ttgcaccagg
    13381 caattgttgc tggaaaaatt tccttggcac tccatctgct gggagtggat gaaaataatt
    13441 tcacacccgg ggagagtgaa aaaacggatg ttttgagtat tttgtctaaa gtaaagaaaa
    13501 aaatcgaaga gtcttttaat cacgtaaaaa gatgtttttt tgatgtggat aaacgggatg
    13561 gttatgggcg cactttgttg tccttggcat tagatgccaa acgccaggaa ctgttaattg
    13621 ccattttagc aaggaatccc attgtacacg caacaacgtt aagatcctca gcctatgttc
    13681 ctttccagcc tattcatcag gctgttgttt tagattatgc agagggcatt accttattag
    13741 ccagcatggg ggcgcaattg acaaatcccc tgggtagtat gagagacact ccggtcattt
    13801 tggctgctcg tttgggcaaa ataaatgcac tggcggcttt actggagtta cccactcaaa
    13861 gtttgtctct tgaatccgaa aacaatcatt tattcgaaga caaacaaact ggccatacag
    13921 cagtggaaga gttatgtgaa cgcatggcca atgagaatga taaagcagag gctcttcgtg
    13981 ggattgccat gctaatatgc cgtggcgctg aaccacctcg caatgaaaaa atgcgaaatt
    14041 tattaagcag caaccgggtt gctttcttaa aagcggtcag tacctatttg gcagataagc
    14101 ctcaattggt cgatgctttt gtagagcgtt gtcatctacg agaaagtgct ttgcataata
    14161 tagtttatgc cgatcattca tgggggagtt ctattcgtca tttactcggt gttcccagtg
    14221 aggcggccct tattgttgaa gaattagtaa cgagaaaata cagtgatcct tcctttgcca
    14281 gcgaaaatgt ttcgtcatta tcaactactg ctacggaaaa tctacgttca gagaggaacc
    14341 cactgaaact ctatgccgaa tttgtcaggc ggtacacgca agcatatgat agccaaatga
    14401 ttaccaatcg ttggagtacc atgcgctgga tgatcgccga agggaattgt gattgggata
    14461 cagtagttcg gtattcgaaa aatcatccaa cgagccgtac caggattgtg ttgaatgaaa
    14521 tgtttaaccc gataccgagg gtacatgagg atattgagtc tcaacagaat tctccaagag
    14581 ttgttctaaa ataatttatg tcaatatttt gatcttgaac cttcgacaga caccttggcg
    14641 gtctgcgaag gtttataaaa catcttgagt atttccagat gattgccaag tacagatagt
    14701 cttttgcttc atttcaatat acaagtcctg aagtatctac ttgcttaaac ttcatatacc
    14761 ccataaaatc ctaaattcat ttgaaattgc taggagtagt acatggggtg gaatgagcca
    14821 gagaagggca aagatccatg gtcgggtaaa aatcaacctc cggatttgga tgaagctctt
    14881 aaacgtatcc aggataaatt gaagaaaaca ttttttggag gcacgggcaa atcgaataat
    14941 aattcgtcag gtggctccag tggcggattg cttgctgtca cagttttatt aatagctttt
    15001 attctttggg ctctttctgg tatttttatt gtggatcctg cagagcaagc tgtgatactc
    15061 cgctttggta aatatgcaga aacagttggt cctggtcctc attggattcc tcgttttatc
    15121 tcatcaaaga ttgttatgaa tgtggatagg gtgctagatt actcctactc cgctcaaatg
    15181 cttaccagtg atgaaaattt agtatcggtc tctttggctg tccagtaccg cattaatgat
    15241 ttgagcgaat atttgtttaa tgtcgcaaat cctgaggaaa gcttacaaca ggcaacttcc
    15301 agcgctttaa ggcaggttgt aggcacgaca accctggatc agattataac tgaaggtcga
    15361 gaagtttggg gaggtagagt acaagaaact ctgacgaaaa ctttagaaag ctataagacc
    15421 ggaattttaa tcgttaatgt gtcaccccaa cctgccagag ctccagaaag tgttcaggat
    15481 gcttttgatg acgcgattaa agctcaagaa gatgaaaaac gtttcaagga acaggcttat
    15541 gcttatgcag caaaagtcgt gcccattgca gaaggtaaag caagtcgtat tcaacaggaa
    15601 gcggaagctt attcaaagca agtcgtttta agggcacagg gagaagtagc ggaattttta
    15661 gccttacttc ctcaatacaa tgcagctcct caagttacgg ctaaacggat gtaccttgaa
    15721 gccatgcaaa aagtaatgaa taagagcagt acgattattg ttgacagtaa agcaggaaat
    15781 ttattgtatc tacctttaga taaactactt ggtaatcaat acgttccgca atctggaaat
    15841 ctaaaagcca taaaaaacgg tacgaatgtc agtaatgagg aggacaactt gattctttct
    15901 ggaagagatc tcacaagacc aacttatagt caagggagag attgagaatg aatgcattta
    15961 aaacgacatt gggtgtctta atctttttgg tactagtgct tttgtttacc actatgttta
    16021 ctgtcacaca agggcagcag ggtattattt tacgtttagg acgtttggta aaagaccctc
    16081 aaactgacgc agtgaaagtg ttaaatcctg ggctccactt taaaacccct tttatcgaaa
    16141 gtgtgcgaat tttcgataca cgcatccaaa ccatggatat caaatcgacc cgtattgtga
    16201 ccaaagagaa aaaagatgtc atggttgact attatgtgaa atggcgaatt tcagatttgg
    16261 cccagtattt taaatcaaca ggtggaaatg aatttaaagc tgaaactttg ctagaacaac
    16321 agttaaatac cttgctcaga gctcaatttg gtaagcgtac catatccgat gccgtttcag
    16381 gagggcgtga tgatgttatg gaaatactgc gtaatgctgc tgagaagcaa gctggtgaat
    16441 taggtattaa agtggttgat gttcgtatta aaggtattga attgccttca aataccagta
    16501 atgccatcta tcaacgcatg agagctgata tgcaaaaaat tgcaaacaga catagagctg
    16561 atggacaagc tgccgcagaa cagatccagg caaaagctga tgctgatgtc acggttttat
    16621 tggctaaaac taatagtaat gctcagcgaa ttagagcagt tggtgaagcg gaagcagcgg
    16681 ctatctattc caaagcatac actcaaaatc ctgatttttt tgcattatac aaaagcttgt
    16741 tagcctatga agccagtttt catagtaaaa aagatatttt aatattggat caaagcagtt
    16801 cattttttga ttatttcaaa caagctatgc caaaaaatga tggtacagcg gctaaaaagt
    16861 aattataatg tgaaattttg ctgtaatcag gttcgatatt tattagcatc atccatctaa
    16921 tataaatgca gttgggtttc tcgtaaaata caactgcatt tttaattttt acaggtatgc
    16981 aaacaaagag taaatcatgg gtaagaatgt tgttgttttg ggtacacaat ggggagatga
    17041 aggcaaaggc aaaattgttg atttgctgac acaagatgct caagtggttg tccgttatca
    17101 agggggccat aatgcaggcc atacattaaa aatcaatgga gtaaaaacag tacttcgttt
    17161 aattccatcc gggatgcttc gccctaacgt aacctgctat attgcaaatg gtgtggtctt
    17221 atcacctcag gcattactta gtgaaattaa ggagcttgag ggtaatggcg ttaatgtgag
    17281 ggagcgatta cgcattagct tggcttgtcc cctgatcctc ccttaccaca ttgctttgga
    17341 taaggctcgt gagacacata tgggcaagtc tgcaattgga acaacaggtc gtggtatagg
    17401 tcctgcttat gaggataaag tggctcggcg cgctttaaga gtaggtgatt tatttcatag
    17461 agaccgattt gccaataaat taacagaatt attggattat cataattttg tattaacaca
    17521 atatttcaaa caaccagctg ttgatttgga atccctattg gatgagtcat tacaatgggc
    17581 tgaagaatta agaccaatgg tttgcgatgt ttctgcctgt ttgcatgaac atagaaaaca
    17641 aggtgaaaac attttattcg aaggggcgca gggtgtttat ttagatattg atcatggaac
    17701 atacccctac gtgacttctt caaatacctg tgtgggatct gtaattaatg gtgcgggatt
    17761 tggtcctcgc tatattgatt acgtgttagg tatcacgaaa gcttacacaa ctcgtgttgg
    17821 tggtggccca ttccccactg agttactgga tgacgttgga aaacgcattg cagaacgagg
    17881 tcaggaattc ggtgcagtaa ctggtaggcc cagacgttgt gggtggtttg acgctgtttt
    17941 attaaagcgc tcgattgagt taaatagcat ttctggactc tgcgttacca aattggatgt
    18001 gcttgatggt ttagaggtat tgcggattgc tgtggcttat aaagacaggg atggcaatat
    18061 attatctcga cctcctctgg cagctgatga tttcaacgat ttgttaccag tctatgagga
    18121 gcttcctggt tggcaggaat ctactgctga tgtgactgtg atgagtgact tacccgccaa
    18181 tgcacgggct tatctaaagc gaatagaaga gatattagga attcctattg atatgctatc
    18241 aacaggacct gaaagggatt caacaattac tttacgaggt ccttttcttt gatacaaaga
    18301 taaagttttg taaccactat gagtgtcatg caaggatttc ttgatatgac actttccgcc
    18361 ttttaagtat tattgtctgt gttttagcaa gctatttcct tggggcagca ggcaattctt
    18421 gcatcgctaa atttcatttc atgctaaagt taatgtacat aaaagataaa ttatctctta
    18481 tctgaatgtt tactcaataa aatggtgaat tatggaatat ctaactccct caggattgat
    18541 tccttttata actgaactca aagcgcttaa tattctacaa caagatgata atactaatat
    18601 tgctctcgta tcagaaattt tagcgcgctg ggttgtagat aaagaatggc tggatgaaaa
    18661 attttactat gtcaatccag aggttggctt taactcctgg ttaattcatg aagaaaaaga
    18721 tcatacttta gcagttaata tcgttgcctg gcagtcagga cgagaaatta ctccgcatga
    18781 tcaccagact tggggtgttg tgggtagcat aataggtgtt gaaaaaaatt atttttggaa
    18841 aagaatggat gatggttcaa agccagggta tgccgagatc ataagagaaa aaacggctgt
    18901 tatacgtccc gctggtgggg taattggttt tttgcctaat gacattcata gtgttattaa
    18961 tgaatcagat gatgtagcaa tttcactgca tgtttatgga aaaaatttga attatacagg
    19021 tcgccatcag tacgacccgg ttaacaattt aatgcaacca tttatagtaa attatagtta
    19081 ggttgatatt tatcattgtc gctatgcttt caatcctgcc aaattgctgt ttactcacac
    19141 agggcttgta aattgatatt aaactctttg ttaaaatata tttttaattc tgtcatttag
    19201 tttatctaaa ttccctcatt aaaaacatgt tttacttcaa gcttgggttt ttcataagcc
    19261 ctgtaaaatt aaaattgtag ctacgaagca aataaatatg gaaattcctt cattaaaata
    19321 tcaagaactt tttgttgatc agatttactt ttaataatga gcaagaccgt gtcgtctccg
    19381 gcaatagttc ctaaaattcc tgaattcgaa gagtctttta tgctgaagga aacgtattta
    19441 cggtctatat aataagcaag actgtttgca ttaccaggat gagtttgcag aatgattagc
    19501 ccaaaatcag aaatctggac atttagaacc agaggtaagc caggctgatt gtaatcaatg
    19561 attttgtaat aaccggatac ttttgcaatt ttcatttttt ttaagcgacg agaaagagtt
    19621 gcttgaggaa tctcataccc tctttttctt aagctgttct gcaaatcact ttgttccgca
    19681 atactttctg tttgcactat cgataaaatc aaatcatcca aaaaggcatc atgggtcaat
    19741 tgctttctcc ctgttatcaa ttcaattgaa tataaattac taaaattgaa attatatcca
    19801 ttttttcttg acagtacaaa tctcgatcat gtatattcaa aaaatgcata aatacgggat
    19861 tcatattcaa tgagaaaagt ttttagttta ttgttaatta gttcaattat agttatatcc
    19921 agttgtagtg agcaaaaaaa tcaaaataca ctgcgttttt ccacttcagc agaatatcca
    19981 ccttttgaat atattgaaaa cggagaaata aaaggatttg acattgattt ggcaaaattg
    20041 atcgccaaag agctaggtaa agaagcagtt tttgacaaca tgcagtttag tgcggttttg
    20101 cctgctgtga attcaggcca ggatgacgtt gcgattgcca caattactat taccgaggaa
    20161 cgtaagaaaa attttgattt ttccaaacct tattattttg aaggaatggc tgcagttttt
    20221 gataattcac agccagtgaa agagatctca caattaacag gaaaaaaaat agcagtccag
    20281 ctaggtagtg ttatggaaat ttggcttcat caaaactttc ctaaagctga aattacggct
    20341 ttggataata acaaccaagc gatagaagct ttaatcgcag gccatgttga tgtggtcctt
    20401 atggatgggg cacaaggggg tgtttatagc aagaaatatc caggactgtc ttattctatc
    20461 ctggcgaaag ctaatgaagg ttatggattg gtcttaaaaa aaggttctcc tctaagggat
    20521 gaaatcaatt ccgcattgga aaatcttaaa gcgaagggtg aaattcaaaa attggaatct
    20581 atttggataa aggggtcaat atgacggaga tagtgcaaaa tctgtttttc attggcaaag
    20641 gaatagttct aactatgcaa ttattaacag gaggtatgct gattggagta ttacttggaa
    20701 ctctcttgtc aatttgcaga catcaaggag tagcttgctt tgttataaac cgattcatat
    20761 ccatattgag aggcacacca ctcattttac aattaagttt tatttatttc gcagtgcctg
    20821 ttttattagg tatgcgacca agtattttag tggctggaat tgttgctttt ggtttgaata
    20881 gctcagctta tttcgccgaa attttaagat ccggaattga gagtttaccc aaagggcaat
    20941 ttgaagcagc ccttactctg caaattccta ccttttactt atggaaagat attattttac
    21001 ctcaagtgat aaaaaatatt tttcccgcaa tgatcaatga aatgatcgcc ttgttaaaag
    21061 aaacggcctt gatttccact attggaggca tggatttaat gcgtcaggct caaactttgg
    21121 cagcacaaca atttacgtac tttatgcctt tatgtatcac gggtttctat tattacagtt
    21181 tggtcttact ccttgaagca ctaggtaaaa aaattgaaaa aagggggcat tatgctgcaa
    21241 gtcattaacg catcgaaaaa aatcggcaat gcccaagtac ttaatgagat taatttaagt
    21301 attcaagcaa acagtattgt tggtttagtg gggccttctg gcagtggaaa aacaacttta
    21361 ctgagatgca tacaacaatt ggagtcaatc gattcaggct ctatccattg tgaaggaaaa
    21421 agcggtttta tgtttcagga ttttcaatta tttccgcaca tgacagtact caataaccta
    21481 atttatgcac catatcttca cgataaaact gctgatcata tggcaaacgc gtgggcttta
    21541 cttgctatgt taggtttgtc agacaaagct cattcttacc cacaaggatt gtctggtgga
    21601 caaaaacaac gcgtggcctt ggctagaagt ttaatgatga aaccggattt attgctttgt
    21661 gatgaaccga cttctggtct agatatggcg accactaatg atgtcattgc tttgttaaat
    21721 acagtgaaga ctctaaaaat cactatgctt atcgcttctc atgatttgtt ttttttatcc
    21781 aatattgcgg agcgattgtt ggttgtaaag caagggcaac taattgctga ctttaaaacc
    21841 caagaggtgt ctgatcctat tttatatcta cagcagtttt accaggggga aatattatga
    21901 aaaaggttat taaaaaaatc gcattagctt attcaggtgg cttggatacc tccatcatga
    21961 tcccttggct taaagagcat tatgagcatg ctgaagtaat cgctgtgatt tgtgatttag
    22021 ggcaacagga agatctcgat accataaaaa ataaagcact aaaaagcggc gcttccaaag
    22081 cgtatgtagt cgatgtgaag aatgagtttg ccattcaata tctttggcct ttggtaaaat
    22141 caggtgcttt atatgaagat caatacattt tgggcactat ttcaaggcca ctaattgctc
    22201 aaaaacttgt tgaaattgct ttgacggagc aagtaaatgc cgttgcacat ggagccaccg
    22261 gtaaagggaa tgatcaagtg cgttttgaat attcgattaa ggcacttgca cctcagcttg
    22321 aaattattgc tccctggaga acttgggata ttaaatcaag acaggaagca attgtgtatg
    22381 ctaaagcgca tggcattgag gtaccagtga ctcccaaagc gccttattct agagatcata
    22441 acatttggta tatttctcat gaaggtggtg tgctcgaaga tccttctcag gaaatgccgg
    22501 atgacgtgtt attaatgact gctcctgtat cacagactcc tgatgaagag gaagtggttg
    22561 ttcttgattt caaaaagggg gttccagtcg ccttaaatgg acaagaactt tctccagtgg
    22621 atctcttaaa ttcattaaat caaaaagcag ggcagcatgg tattggagtg gcggacattg
    22681 ttgaaaatcg attagttgga atgaaaattc gagggattta tgaagcacct gctgctgccg
    22741 tgctttataa ggcacataaa cttttggaga gtttatgtct cactcgttct acgttacatc
    22801 tgaagcaatc tttgcagcaa acttatgcca atttggttta tgaaggaaga tggttttctc
    22861 aaaccaaaca ggctttggat gcattcattg atgtgacgca gcagcatgcc acaggctgtg
    22921 ttaagttaaa gttattcaaa ggaaacatta tccctgccgg tatgcattct ccctatagct
    22981 tgcatcatcc cgaattggct acttttgaag aggataatgt atacaaccaa aaggacgctg
    23041 aaggattcat taacctcttt tctttatccg ctaaaatcta tagccaggta catcaggagg
    23101 ggaattatga ctaataagac atggggaggg cgatttaaga aatctttgga tagcggtgtc
    23161 aaccagttta atgcgtcatt gtcttttgat catgtgcttt ttgaccaaga cattaatggc
    23221 agtcaggttc atgtgaagca attagctaaa cagaagattt taacagaagt tgaaagccag
    23281 gcgatttatt ctgctcttga agaaatcaga agcgagataa aacaaggcca atattctttt
    23341 aatgtacgtg atgaagaaga catacatatg tttattgagc aattactgat tcaaaaaata
    23401 ggcgatttgg gtaagaaatt acacaccggc cgtagtcgta atgatcaagt ggctttggat
    23461 cttcgtttat ataccagaga taaaggctgt ctgattaatg aacttttgac aaggcttatc
    23521 gattgtctag atgatctaac cagtaaacat caacaggatt taatgccagg ctatactcat
    23581 ttacagcaag cccaacctgt tacgttaggg gcttatttta acgcgtatca atgcatgttt
    23641 ggccgagata aaagcagatt ggaagattgg tttaaacgca tgaattattc tcctttgggt
    23701 gctggggcac tggctggaag taccttgcct cttgatagag aatgggtagc cgaatccttg
    23761 ggatttgcag ggatcattcc taatactctt gatgcagtca gtgacagaga ttttgtcatc
    23821 gagttatgca gtgttgctgc aatgattatg atgcatcttt ccagattatg tgaagatttg
    23881 attctatggt ctacccagga atttaatttt gttatattgg atgatgcgtt tgctacaggc
    23941 tcttcactga tgcctaacaa gaaaaatccc gatgttcctg aattgattcg tggcaaatca
    24001 ggacgagttt atggtcatct aatggccatt ttaacggtaa tgaaagggtt gcccttggct
    24061 tataacaaag acatgcagga ggataaagaa ggattatttg atacgattaa tacgataatc
    24121 gcttgcctgc aaatgataac tcctttcttg caaagtctga cttttaatac cctgttaatg
    24181 aaaaccaaag ctcaaagtgg ttaccttgat gcgactgcca ttctggagtc attagtgatg
    24241 aagggtatgc cttttcgtga tgcacaccat caggttgggg catggattgc agaagcgata
    24301 gaaaaacaat gttcactgaa tgaattatta aaaggaggct aagaatgtct ttttccacct
    24361 caaccaattt gactccaatt gaaacgcaag acgatgacct tgaaaaaatt caatctcaga
    24421 ataacccatc tgcaattaat gaagtgaaga ccaatacttt tgtcattaat ttacaacaag
    24481 ctatttttgg cttggagaca ggtagtgagg ccgttgattt tgattctcat aagaatacag
    24541 gctttccatt tcctgctggt gaatacaaac caagacattt tcttactggc acagagttag
    24601 caagggagga aattcagttt atcttaagcc aggctcgctc tctaaaacaa aacccgggat
    24661 tatataatca ggcacttgcc aataaaagtt tggtaatgat ttttgaaaaa ccttccttta
    24721 gaacccgttt gagttttgct ttagcgattc agagtatggg ggggatcgtt gttgaaagca
    24781 ttgctaactc aagaaagcag gagactccag ctgatatggc tagagtttta aatggttatg
    24841 ctgattttgt aatggtcaga acacatgagg acagtttttt gcaagaaatg gccgaatatt
    24901 ccaaaatccc tattattaat ggcctctccg cattgcatca cccttgtcag actcttgctg
    24961 acttgttaag tcttgaagaa caatttggtg ctttggaagg attaaccctc acttacatag
    25021 gggatggcaa taatattttg catagtttga tgcttttggc accgcaatta ggtgtcactg
    25081 tgcattattg ttgcccaaaa tcaaatcagc ctaatgaggc cattattaag gaagcccaaa
    25141 gcaagacagg gaaacaaatg atttattgtt ttgataaacc ggaggaagct gtgaagcaag
    25201 cggatgccgt ttataccgat gtgtggacaa gtatgggatt tgataatcct gaagcgggca
    25261 agatttttaa agggtttcag gttaatgaga cattaatgtc tcatgcaaaa cccgattcgg
    25321 tgtttatgca ttgtatgcct atggaaaggg gaaaagaggt ttctgccact ttacccgaca
    25381 gcgcgtcatc tatcattttt aggcagagtg agaatagaat gcatgtacaa aaggctttga
    25441 tgctattttt ggatcaatag aactttggat tatgaggccc atatttgttc atgatgaaac
    25501 gaaattgcgc atttctcctg atatgggctc atgatttttg caaattaatt tagtaattct
    25561 tctcaaaggc aattaattgt tgttccagac tccattgcaa ttttgccttt tcgttgtgct
    25621 cgatgaattt ttgacagctt ttgcttccaa ggtcatggca atcaggaatc aattcagctt
    25681 tatccgcatt agcccatatg ctttttccag gtaagaatga gtaagtcagt gtatttctat
    25741 taatttgttt taattgctga taattaaggc catgttcttt ggctgcctct acatattgtt
    25801 gggttaagtc cgttcttagc actccttcgt catcagtgga taaaacgact ggtacattat
    25861 gagtaagata ataattaaga ggatgattgc ggccggatat attcagaatt tttaaattac
    25921 taatcaagtt tatttcaacg ggtatgtgat ggctagccat gtattttaag gtgtcttctg
    25981 cattgttttc ataaccgata tccacaccgt ggccaattct ttgggcgtgt ccaatcagca
    26041 gtgcatcgcg aatatgatta cttagatttt ctggtgtaac tgcttgagga gataattctc
    26101 cggcatgcaa ggtaatagga acattcgggt atattcgatg cagatattca aagatggaca
    26161 tgtgcttgtg gtaatcacgc aaagaaataa ttccatcttc cggttgcacg aggtttacgc
    26221 cgattaagtt ccccttagac ttggagacgg cttcaaaggc attcaaggct tgggcaaaga
    26281 cattatcaac gggctgctct ctgagtacat aataaataaa tttaattttt acatggcagg
    26341 aatcggaaga agggttatct ttgcagccta attcttgaga tgctttggta agaattcggt
    26401 cgctttccaa aacagtattt tttatgttgc tttgaaaatc tttattcgct aacaacaggt
    26461 gccttttctg gtcatatgat ggaatgtcct tgattaaatc accataagtt aatgagtggg
    26521 cattgtccgg gattatcata atttccatgt attgctcttt ttgttgtgct gctcgctgaa
    26581 caatatcggc aagcaactga ggcctataat caaaaactat gggcatatat ttgacaaatc
    26641 cattgaaaaa atgatcgtgg ccagattctt taccaggtat aaagtctttc atagaccagt
    26701 ctttaattat gttggaatag agttcaggat tgttaaaaac ctctttaaca ttaactccct
    26761 cacaatgaga agaatcttta cttacggcaa atgagtgttt atcgaggcaa tagttgccat
    26821 ttgcggcaag tgccagcatg gtctcaggat atgctcctcc agccaaatga taatgtaact
    26881 cgccaccctt gggcattgtt ttaaaaaagg cataaagggc actggaatcc ttttttatat
    26941 gactaaaata ggaatcaaca ctggcataaa ttgggcacaa aaggaagaaa ctggtaaaaa
    27001 atgcgagtaa tctggtaatg ttcataattt caatgtgatc attaattggg cagatattat
    27061 ctcataatct tgcacgattc ccaacagaat atctgtttta tcatgcaatc tcttatgatt
    27121 ttttacgaaa tgataatgat tgtatttttt tattattcat tgcagaatgt ttttaaggtt
    27181 taacacatgg aagaaaaaaa tgccctattt ctatacaaga tcagcgcctt tgttttttgt
    27241 attcgttatc gcacttattt ttataaattt ttcctatgcc catgaatttc aaactgattc
    27301 aaaaaataaa cataaagtgg atgttcacat aggtatttat gctccgtttt ccagtcattc
    27361 agcttttatt ggaagaaata ttctggctgc aatggaaatg ggcagcgaac ggatacagtc
    27421 ctccgatatt cattattcat tttatactct ggatcaactt cctaaaaata agaatgctgg
    27481 tcttgtgctg caaaaattta ttgatatcca tcatatcaat gtgttggtga ccggaggtaa
    27541 agaaacgggg ttaatagcag ctcccatagc aaagaaaaac aatttgattc attttagtct
    27601 gaccaacaat actgaagttg cagacggcag aaataatttt atcgtctgga gtccagctta
    27661 tgaccaagcc aaggttttgg ttaacgaact aaaacgtcag caggtccatc acttgggaat
    27721 tattacggca aatcaaccgt cctcaaccgc tttgacacat agtgttataa aacaattgca
    27781 ggctgattct catattgatg tggctgcctc tgagtatttt gaacctggaa ataaggattt
    27841 ttcaagatta actaacagaa ttaaaagaaa aaatgcagat atgtatttgg taatggcatc
    27901 acctgaggat attaaattaa tacaagaaca aatggaggca gataaacagc atgtgcccat
    27961 taccagtata gttgagcgtg ctactccaga agtggtaaag gtatttgaag gtcaatggta
    28021 tgttgattct catgagatgc agcctgattt tattaatgaa tttaaaaaaa attatttaaa
    28081 ttatcctgta actgaggcag gttatgcgtt tgatttattt cagcttttga acaaaagcat
    28141 agtaatggcg ataaaaacac aaggttctcc tacaagccag gaagtcgttc atcaactcca
    28201 tgacattgcc aatggaacag gcgtaatggg aactatggta ttggacagta agggttcttt
    28261 atttaccaaa tctgaagtga aaactgtgaa aaatggcgag attcatactg tctaggtaga
    28321 aaataaaacc tgaaatcatc agttcattgt gaatcgatga tttcaggcgg gaggactaat
    28381 tggtcattat ctgttagcta acgccattcc ttctaatacg gtgagtgccg catataactg
    28441 ataatcctcg tgtattaaat ctcctgtttg aatttggttg tttttggatt cagtgttttc
    28501 tgaattgtct ttgttaatca agtgcccatt aaggtccgct tcagtaaaac cagccagggt
    28561 gtcttttttg actgcatttt taggaacgtc cacttcttcc acaacgatgt caggaattat
    28621 tcctttggct tgaatggaga ctcctgatgg agtgtaataa agtgctgtgg ttagttttat
    28681 tccggttttg ccatcaagag gtaatacggt ttgaacggat ccttttccaa agctctttgt
    28741 accaaggatt atagctcttt tattatcttt taatgcccca gcgacaattt ctgaagcaga
    28801 ggcagaaccg ttattaatta agacaacgat aggcgcattg tctaacacat cccctggatt
    28861 ggccagggcg gtgaatttag agccaggtag tctgccttcc gtatagacta tcatttcttg
    28921 cttacctgcc ttgtcattgc caagaaaagc atcggatacc tggatagctg aatcaagtaa
    28981 acccccgggg ttatttctta aatccaatac taatcctttt aatttcccgc ctgcttgttg
    29041 ttttaattgt tcgattgctt taatcatatc ttttccagtt aaagcctgaa attgagtcaa
    29101 tcgaatgtaa ccataacctt cgcttagcat tttgcttttt acacttttta tttgaataac
    29161 ttcgcgaatt aagtcaaaag ttaaaggttt gtttacacct tttctcaaga tagttaattc
    29221 tatggtagta cctggtttgc cgcgcattaa attaacagcg tctttaagag aaagaccttg
    29281 aactgactct ttacccagtt taataatata atcgccagat ttgattcccg ctttaaatgc
    29341 tggagtgtct acaagaggag tgattacttt aacaacacct tcttccatgg tcacttcgat
    29401 acctaaacca ccaaattcac cgctggttga tgtttgcaac tccttgaatt cttcttcatt
    29461 gagataactg gaatgcggat caagaccggt tagcatcccg cgaattgcgt tatcaaataa
    29521 ttctttatca tcaactggtt tgacatagta cttctttatt tcaccgatgg cattggaaaa
    29581 ccgttgcaca tcttccaacg gaattcgttt tgtattagaa taatttgaat tggtttcttc
    29641 agccgaaaac gcggtcaaag gcagcatcag agttagggca taaaccaatg ccaaagtact
    29701 ggaaaaaagt ttcttaataa ccatagcgat ctcctcagat gcaaggccat gaaattagcc
    29761 acctgcgtat ttttttatgc aatttaaatg ccagcggcta agctttaaga caaccagtct
    29821 aacggattga ccgcttttcc tctttgtctt atctcaaagt atagaccgtt ttgcttaatt
    29881 ccaccggtat ggcctacact tgcgacttgc tcgttttgtt ggacgatttg tcctttgcgt
    29941 ttgaagagag attgattgtg tgcgtataaa gtcataaacc cttggccatg gtcaataatc
    30001 agaagcaaac cataaccttt tagccaatca ctaaacacaa ctttccctgg gtaaacggca
    30061 gtaactatgg agccttcatc ggcaaaaaat gtcactcctt gattcattct gcgtaaagat
    30121 ctatgggtgg tttggacagg taacggcaat tttttgcgca ttcccgtgaa cggtttgccg
    30181 ctttgaatta cactttgttg agataatgac ttcatgagat gagataagtt gtctctatct
    30241 ttttgaaaat cttttaatga ttgttgatta ttttgaatgt cattatcaat ggattgaatc
    30301 agagtgctat ggtatctttt attttgctct agctgttttt gattttgtga caatttaaat
    30361 tgtagctgct tgttttctgc aagttcattt ccaagcttgg ttttactgtc gttaagattt
    30421 tgtcgggttc gagcaatttc atcaattaac tgctgacgtg atcttattat atactgataa
    30481 taggttaaaa ttcggttgat cttaaaaggc tcatcttggt taatcaacca tttcaaaggt
    30541 tggtattccc ccatctgata acgtgccctg acatggtttg ccagcatttc ctgttgagct
    30601 tgcaatcgtt catttaagtg atttattttt ccttgcagct cggcaatgct tttttctttt
    30661 gcggacatat ttgcttgtat gattcttagc ttgcgtattc cttctccaat gtgtttttca
    30721 gtgctgccca gctcttggtt tagcacagtc cgtttatcct gtgcattggc taaaacttgc
    30781 tttagtttgt taatcttggc atctaactgt tttagtttgg tttgtgcttg agttatagaa
    30841 tttggagcag cttttgcctc aaggccatta caaaaaaata gaatgaccat aagtgccaaa
    30901 ccaagaggag cattggacaa atagttggac aggctagaca tgcgcatggt ttttttccag
    30961 aaggatacga ccagtcattt ccggaggcgg agttatccct aataatgcaa gtaaggaagg
    31021 tgctatatcg attaaacttc cttcagcatg agtgaagtgc caccccccac ctacataaag
    31081 aaaaggaacc ggttcactag tatgagctgt atgagcttga tttgtcgctt cgctaaacat
    31141 ttcctcagca tttccatgat cagcagtgat caataattta cccccattgt ttttaagggc
    31201 ttgccaaacc tgatgcatgc actgatcaag gcattcaata gcttgaacgg tagcttcaaa
    31261 attccctgtg tgtcccacca tgtcagcatt ggcataatta caaataatga catgataatc
    31321 tcggctattg atggcggcta ttagagtttt agttaattca ggtgcgctca tttctggttg
    31381 cagatcatat gttgccactt gcggagaggg taccataatg cgctcttcat ttgtaaaaac
    31441 actttcacat ccgccattaa agaaaaaagt aacgtgagca tatttttcag tttcagctat
    31501 gcgtaattgg ctgagaccat gagaagacaa gacctcaccc agggtattat ttaaaggaac
    31561 aggtggaaat gctgtcgtgg taaataaatt tttatcgtat tgggtcatgg agacaaaata
    31621 ggacagcttt ggcagtgttt ttctttcaaa tcctttaaac aaagggtcaa ggaatgtaga
    31681 ggttaattgc cttgctctgt cagcacgaaa attgaaaaac aggacagcat ctccatcttg
    31741 tatagaatgc tcttttccta taagagtggg tgggacaaac tcatcagaaa ttttattttt
    31801 gtaatagaaa tgtattgcag tttctgcatc aggaaattgg tgctcacttt tgccttgtgt
    31861 taataaatta taaacaggct ctactctctc ccaacgtttg tctctgtcca tcgcataata
    31921 tcggccacaa attgaattaa tggttgcaac agggtgttta accagttctt cattcaggca
    31981 tttcaagcta tcaagagcac tttgtggtgg agtatctcgg ccatcaagaa acaaatgcag
    32041 atggacggat ctgaattttt tttgattaca aagggcaagt aaagcaaaca aatgttgttc
    32101 gtgactgtgc actccgccgg gagagagtaa ccccatgatg tgaagagatt tttctgtttt
    32161 ctttaattgg tcaattactt catggaaaac agcattttta gcgaattttc cgttatttat
    32221 cgcttcatta atgcgagtaa aatcttgctg tatcactcgg cccgcgccaa tgtgcatatg
    32281 cccaacttct gaattaccca tttgctcatc aggtaaacca acaggcaagc cggaggcttt
    32341 caaaagaata tgagggcagg tttgccacca ttcatcccac tgaggggtat tggcttttgc
    32401 aatagcattg tatctatcat tttcgttata gccccagcca tcaagaatca tgagtaccag
    32461 gggagcatta tgcgacatga cctaaatcct aaattgtaca atgaaagggc taaaatgata
    32521 attatacctt cgcggcagta agaggtatat agtttcagta agagttcgct gttattaaat
    32581 ttagtagcaa tcttcttttt gttcgcagtg accataagca tcgcatgact ggatcggtcg
    32641 acacccaggg taataaataa tgggtacggg agttgtaata attaaaggag gagtagttga
    32701 tttatcacag gacttacaat gattaaaagt agctcttaca tcattgtctt tgggtagatt
    32761 ttttggattt ggtcctatcc ctaatagatt gaacgccatg gcatggttaa caagagcagt
    32821 taagctgaca aataaaataa ttgctttgag gatcaggttc ttagtatccc tactcatgat
    32881 gtgaactcct ttttaatttt atgccccgta ttatgcgata ataagagtat agttgcttgt
    32941 tatggtcctg ctcctatacc aaaggattat cttgcaattc ttccggtaca tattgagaca
    33001 aatatttgct taaactcaat tgcacggtcg aggttatccc atattgcgtt tttaacaata
    33061 ataaagtact ccagccccac caactgtttg tccaatgcct gtcataagga tttgtcaaat
    33121 tggttcttct taacaactgt ttgttttctt tatcccaata gaacatagga gcaatatcca
    33181 tgttattttc atcatttaat ttggtgatgt gtggaatcat gtcctctatt tgattttttt
    33241 ctggtagaac gcctgagtaa taagcttcaa atatttcatc agcggaagct tgcaaggcct
    33301 ttaagagcat ctgctggttg gtttgattaa aaggattaaa ataagaatag tcaccataaa
    33361 ccatccattg ttctcccaga ttattcgtca catgaattcc atacttattt tcttcattat
    33421 gcatataatt ggataacaac gcaccaagga ccgttggtgt gataatggct gataattctt
    33481 ctctaggggt gcgcaaatgg ccggcagcaa agtgatctga taaatagtgg caggcaaaag
    33541 catccatcgc ataggcgatt tccaaatcag agcgttttcc tgttcgtttt gcttttaatg
    33601 cctgttgcag tgcgacatgg tggccacttt tataggctat cagattattt ggcgtaaagt
    33661 gatcatagtt ttccatggct agttttagat atctgccagg atttaaccac caggtcaggc
    33721 tattacaacc acctcccgtg atgcagttga tttggcgacc tacttcattt ccaattcgtt
    33781 tataaatggc ttcagcagtt tctccttttg caatccctga ttcgacttcg cgcatttctg
    33841 ctctgataac ggaattaaga tcttctacct cactaactgc aatggcattc ctggcaaaac
    33901 tgttgaaaac ctctttaaaa cgagtttgtt tttccagttt tgttaagcca tgactaattg
    33961 gttttcctac aattccatac agatcaccaa gacttatgat atcgccggct gttagtgcca
    34021 atccattttt taaatgtaat acaacacccg attggggaat ctcgtttatg tcaaatcgta
    34081 gtttgatttg ttgccccatg gaccaatgct cggacaaagc aaaactgccg ttcgattcac
    34141 cataagttat agaggaaaaa gccaacaagc taatgctcat tgcaaataac cggggcttta
    34201 ttaatcgatg gatagatttt ttcattatgt cctcatcaac agggattgat cattggtttt
    34261 agtgagaaat agcgtattgt ataacgtttg agatgaaatg ttgatgcatt ggttcgtccc
    34321 gagataatgc ttaggtgtga atggagtctg tacaaataac cgtaaccaaa aacgacatag
    34381 agcgctatag gtcatgggaa ttatctcctt tctgggtatt catgatgaaa agtgacaact
    34441 cgttacgaag attttttcag gtttaataat gaggtgtttc ataaacaagg tcaattgatt
    34501 tcttaagtag tacttaaatt tttatctcaa agaggttaga ctatgcatca tttttattag
    34561 tcggataagt tgttttgaat cataaattac ctcaacacat tgccgttgtc atggatggaa
    34621 acgggcgttg ggctgaaagc cgcggtttgc cccgtgtaga gggacacaaa gcgggccttg
    34681 actcagttaa aaagattatc aattgttgcc tggaaaaaaa aatttcctgc ttaagcctgt
    34741 ttgcatttag ttctgaaaat tggtcaagac cagtgacaga agtcaatttt cttatggaat
    34801 tgtttttgga ggctttgcga aaagagatag acgatttaaa ccagcatgga atacggttaa
    34861 agtttactgg cgatagagaa cctttgtctc aagtattaca aaagcaaatg tgtgatgcgg
    34921 aagctcttac caagaataat caacaattaa ttctcaatgt tgttgtgaat tatggtggga
    34981 aatgggatat agtgacggcg gctagaaaat taatacgcac ggttttggac gggaagttgg
    35041 catatgatga aatcaatgag gcagtattcg ctcaatttct tgatacgaat ggcatgccgg
    35101 aacctgatct gtttattcga actagtggtg aattaaggat aagtaatttt tttctttggc
    35161 agttagctta tactgagctg tattttactg atgttcattg gcctgatttt aatgagcatg
    35221 agtttgaatt ggcattgact tcttttgcca ggcgggcaag aaggtttggg caaatttcac
    35281 aatctgaata aaggtaaaac catgtttatg caacgattga tcacgacatt aatattagcg
    35341 cccctggttt tgctagctat tttttatgct ccttcctgga ttttatgggg tattgttcta
    35401 gttatactcc tgcttgcagg actggaatgt ttcaaattga ttcctgttga caatttaact
    35461 gtacaggctt gttttttgat atttttattg atgtgctttt gggccagtgg ctatttctat
    35521 ccctattggt tgagtgtggg cttattggtt tggttattaa atgggttagc aattcttact
    35581 tatccgcgtt cacaaaatta ttggggttat ccaatcgttg ttgccatagc ttctttgata
    35641 ttactaccct tatttactca aagtcttatt caaatttctt ttttacagca aggaaaggaa
    35701 ttacttgtct atttgctttt tttaatttgg gcttctgata ttggtgctta tctcgctgga
    35761 aaacaatggg gaaaacataa attaatccct aaagttagtc cgggtaaatc ttgggaaggt
    35821 gttacaggcg gagttatttt agccatgata gtcgcgactt tgggctatta ttattttata
    35881 ccttactcaa tttatatatg gtttggttta gcattgtcta ctgtagttat ttcaattttt
    35941 ggtgatttgt tcatcagtat attaaaacgg cgttgtcaat taaaagatac tggaaatatt
    36001 attccaggtc atggcggtat attagatcga ctggatagcc taattgccgc gttaccatgg
    36061 ttttactttg gattaaccta cataccactg ggaatttaat tttctggtcg tatcaatctt
    36121 tggataacga tatgctttca acgttgctgt actttttatt agcattggta ttgttagtca
    36181 ccatacatga atacggccac tttcaagtag cgcgatggtg tggtgtgaaa gtgttaaggt
    36241 tttcatttgg ctttggaaag attcttgcgc gtttttatga taagaaagga acagagtatg
    36301 cctggtcttt gtttcctttg ggtggctatg tcaaaatgct tgatgaaaca gaaggagaag
    36361 tttctgaaaa agaaaagcct ttcgcattca ataatcaatc tgttcttgta aggatagcta
    36421 ttgtagtagc agggccttta ttcaatttta tttttgcgtt tgtagcattg tggttggttt
    36481 tggttatcgg tatgcactct ttggctccga tgattgagtc agtaaaacca aatagtattg
    36541 ctgcacgagc tggcctactc cccaagcagg aaatccttgc tcttaatgga gttaaaatta
    36601 atagttggcg agattttcaa tatgaaatta tgcctcttat cggctctcag gaaacagtga
    36661 gcttgactgt aaagtcaatg ataaatggtg aagaaagcac gttgtatttg cctttggata
    36721 agtggcagtt ggatgataaa aagcctgatc ctattaaaag cctgggcatc gttcctttta
    36781 tacccactat tccacccata gtcggtgaag tggttcccga ttctcccgct gaaaaagcag
    36841 gcttaaaaat aggggatgaa attattagtg tcaatggtca gcatttcaat gattggttgt
    36901 atttagtcag ctatgtaagg gaaaggccca attctcaaat caatttggat attaaacgcc
    36961 aaggtaaatt gctgaagata acggtacaca ctggaagtca ggataataat ggaaaattag
    37021 aggggttaat aggagttaga tctcagaaag tagattggcc tgctcattgg ctacggctgg
    37081 agcaacagcc gccaatttca gcacttggta cggcttttaa gcaaacaata caactcacag
    37141 gcacaacttt tatcttaatg ggaagattag tgactggaaa attgggatta aacagtatta
    37201 gtggtcccgt aggtatagcc cagggggccg gtgattccgg acgcggagga ttggtatcct
    37261 atttattttt tctggcatta gtaagcatta gcttgggagc gcttaattta ttaccaatcc
    37321 ctatgctgga tggcggtcat cttttgtatt atgtcttgga aatcataaga agaaagccac
    37381 tatcggacgg agttaaatct gtagggatct attttggatt attactgttg gttgccttaa
    37441 tgtttgttgc tcttagtaat gatatatcaa gattaaccag ttaggatttg gcgagagtaa
    37501 ttctaatttc tgtatgatta atgaatgaaa tgatgtttta gcgacaaaga aacttgacag
    37561 tagtttctag ttcctataaa agggtttcaa ttttttgtac aattgaatgg tttttaattt
    37621 cataagtgcg cgtagtgctg gacgtaaaat aataatgaaa aaagtcagta ataaattaat
    37681 attaggtgtt tgttgttcta ctcttttagc ttggtcatcc caatcctttt cttctgatac
    37741 ctttattgtc aaagggatta gagttaacgg gttacaaagg gtttcgacag gtacggtatt
    37801 aaactatatg cctgtgcaag tgggtgagga aatcagttcc agctcaacag ctcaaattat
    37861 ccgcgctctc tatgagacag gatttttcca gtccgtttcg cttgaacgcc aagggaatgt
    37921 gttggtggtc aatgtgatag agcgagcaac tattggctct attaccgttg taggaaataa
    37981 ggaaataccc tctgataaaa tgaaggcttt tcttaaagaa atgggtctgg tcaaaggcag
    38041 agtatttcaa agatcttcct tggagcgttt ggagaaggag ctgaaacagg cctacacagc
    38101 cagagggaag tataattctc gcattgaaac taaagtaact cctcttactg aaaatagagt
    38161 agccattagt attactgtat cagaaggtcg agtttcacgg attaaagaaa taaaaataat
    38221 tggtaaccat gattttaaag caaatgagtt attgcctgaa ttgacgttaa gtacaagcaa
    38281 tctgtttact tattttacta aaaaagatca atattccaaa ccaggaatgg atgcttcttt
    38341 agaagcatta cgttcatttt atttagatag gggatattta aaatttaatg ttgtctcttc
    38401 gcaagttttg ctatcgcctg ataaaaaaga cgtctatatc aatattcata tagaagaagg
    38461 acctcaatat catttctcag gttatgatgt ggttggaaaa acgatattac ctaaagaaaa
    38521 aattgattca ctgatccagg ttaagaaagg cgatgttttt tctcgtaaaa aggttactga
    38581 atccatctct gcaatagggt tagctttagg ggatgtagga tatggttttc ccgcgattaa
    38641 tgccgaacct cgaatagatg aaaataataa aacagtattt attacttttg tagtgcagcc
    38701 gggccgtcat gtttatgtga gacgtattaa ttttcatggt aatacgaaga caggagatta
    38761 tgttctacgt aatgtgattc gtcaggatga aggtgggcta ctgtctttac ataacatcaa
    38821 ggaatctgag cgtcagttac gaatgctggg ttatctaaaa aatattgatg tcaaaacgac
    38881 gcctgttcca ggaaccaata atcaggtcga tctggatgtt aatgtggaag aagctccttc
    38941 agcagaagca agcgcatcca tgggatatgg tacaaacggt tatcaattta atgcatccgt
    39001 taaccaacgc aattttatgg gaagtgggcg ttccatgggg gctgctttca atgcaagtca
    39061 atgggggcaa gactactctt ttaactatta taatccgttc tataccgata ctggggtagg
    39121 ccggggagga agcttatatt attcaaggat tgatcctaaa aatttaaatg tcagtacata
    39181 cagttctaat cgctatggtg gcgacatcag ctataatttc ccgttaggtg agaaaagcag
    39241 ttttcaattg ggatatggtt atcaagatat caatattaag tcagtgggtt acgtactccc
    39301 cataatcaac ttcgttgctt taaacggtaa tcattttcag gaaatcaggt tgacatccgg
    39361 ttggagtaga aacagctatg atcaaatgcc ttatcctaac caaggattta atcaacaagc
    39421 tattgctatg gtcgcattgc cagcaacatc gcaatctcta tcttactata agagctctta
    39481 tcaggcgcat ttatattatc ctctaacccg cggctggatt ttttctgtcc tgggtaatgt
    39541 ggggtacgga aatacttttg ataatttcgg attacccttt tttgaaaact actatgccgg
    39601 tggtccagtg caaccaggcc aggttcgagg gtacgatagt tattctttag gcccgcaaga
    39661 taattttgga aatgcaatgg gtgcgaattt ccttgtgaat ggaagtgtag gcctgatact
    39721 accataccca ttgagtcgag ataatgtaag aacgactatt tttgctgatg cgggtaatgt
    39781 atttgcttcc gggacacctc ctgctttacg tggaactccg gcaggtccga tgcgttattc
    39841 agctggtgtg tcattagaat ggcgctcacc ttttggtcca ttgtctttta gcttggctaa
    39901 ggcattgaat ccacagccat tggatcagac tcaactgttc caatttgctc tttcctcggg
    39961 tttttagagg tattatggat tagagtaagc ggattttagt attttaaaaa ctagtgctgt
    40021 acatatcgca acctttttcg aattgtgcta gcatttcaaa ctttaaatta ggagatcatt
    40081 atgaaacgtc tgggtgcggt gttagtcgca ttatttttgg gtttgttcgg cacgactgtg
    40141 tttgctgatt gggcaaagat aggtgtagtt gatttacaaa aaatcatgca aacctcaaat
    40201 caaatgaagg aaatccaaca gaagttggaa aaagaattta agcctcgtcg agacaaactt
    40261 gtcgcaatgg aagcaagttt gaaaagcgac atggaaaaat ttaagcgtga tagtgctata
    40321 atgagcgcga gccaaaagaa agaattggaa aagaaaattg ttgcgtcaca acaacagttt
    40381 gaacgtgatg gacaacaata tcagcaagaa ttgagcacgg ctcacaatga agctatggaa
    40441 ggtttgtata ataaggttcg tactgccatt accaaaattg ccaaggatga gaagtacgat
    40501 attattgttc agaaagaagc agcgcccttc agcagcgaat ctcttgatgt aacagataaa
    40561 gttatcaaag caattaatta atttggcgga tgcgttgcta accttagagg aactggcaaa
    40621 ctatttaaat ggcgtctggc atggcaatgc caatcacgcc atttttagtc tatcctcgtt
    40681 ggctcgagct tcatctaagg atcttgcgta ttttgataat cccttattgc agtctgtctt
    40741 aaactctacc agagcaggtg ctgttttatt aaagcccgag cacaaagatt ggtgtcctgt
    40801 gaactgtatt gtagttccca atccgtcaga agcaatgttt aaagcagcaa agagcctgcc
    40861 tcaccttgct accaatgttt gcggaattca tcctacagct caaatccaca aatctgccca
    40921 aataggtcaa tacgtatcag ttggcgctaa cagcgtgatt ggtgaaaatg tacaattgga
    40981 tgattacgta actattggtt ctaatacgac cattgaatct tcagtactga ttggcagagg
    41041 gagtcagctg ggttctggag caataattca ttctggtaca gttttgggcc aatctgtaat
    41101 tattgattct gggtgtattg ttggtgctgc cccgtttaat tgctacaagg aacatggtgt
    41161 ctggcaacaa gcaccaaact ttggtggagt tgtaataggc caaagaactc aaatcggtgc
    41221 aaatacagta attcacagag gctccattgg cgatacttat ttaggcgatg gagtctgtat
    41281 agacagttta gtactcatcg cgcatgatgt ttatataggg aataacactg ctattgcagg
    41341 ttctgctgca attggtgctt tagtgcaaat aggcagtgat tgtatcatag gtggggcaag
    41401 ttgccttgca gcgaacattc gtttgactaa tgatgtagta ataacaggaa tgtctacggt
    41461 gacaaaatcc atcgctcgcc ccgggattta ttcttcggga acgacggttc acgatcatcg
    41521 caagtggcgt cgtaacacag cacggttcag gcatttggat gactacatta caaagctgat
    41581 aacaatagaa aaaaaattga ataataacaa ctaattatgt tttatcaatt tgaagtaaca
    41641 ttatttttga aatttttaat tgattagaat gagctaaacc aagataatga gccattttgt
    41701 aatgcaatag gcgattaatg agaactaggg tatgaacgaa tcgatagata taaatcaaat
    41761 ttttactttg ttaccacacc gctacccatt tattctggtt gatagagtga tagattacaa
    41821 agtaatggaa tatttgattg caatcaaaaa tgtgactatt aatgagaact tctttaccgg
    41881 gcattttcca ggaaatccta ttatgcctgg ggtactaatg cttgaggcct tagcccaagc
    41941 ttgtggcatt ttagccaatt tgtctcgcca acccaaagaa gggtatgagt ttctgcatta
    42001 ttttgctggt attgacaatg ctcggttcaa gcaagtagtg attcccgggg atcagttgcg
    42061 attagaggtg agactggctg gacaaaaaag agatttttgg cgtatgcacg gagaagctta
    42121 tattggagac aaattggctt gttccgcaga cttattaagc gcagcaaagg aaattaaaaa
    42181 gtgatagatg aaagagcaat gattcaccca tcggcgaagc tggcaagcgg agtgtcaata
    42241 ggccctggga ccgtgatagg cgctgatgtt gagattggtg aaaatacctg gatagggccg
    42301 catgtggtaa ttgaaggacc tactgtaatt ggtaaaaaca ataagatctt tcaatttgct
    42361 tccgtaggag atgagccaca agatatcact tataagggcg agccaactcg tcttgaaatc
    42421 ggcgataata atgtaatcag agaatattgc atgattagcc gaggtactgt caaaggtgga
    42481 ggtgtcactc gcattggcaa tagcaattat ttaatggctt attcacatat tggccatgat
    42541 tgtatggtag gcaatcatat tattatggtc aattatgcag ccttatcagg tcatgtcaca
    42601 attaatgatt acgcaattat cggcccttat gctgcagttc accaattttg tcaggtggga
    42661 gcctacgcct tcatagcccg agctacctat gtcactaaag acgtattgcc ctatgttatg
    42721 attgcaggtc ataccacttc tgcttgtgga atcaacactg ttggtttaag aaggagaggt
    42781 ttttcttctg ctgcgattga ttgtttgcga cgcgcatata aaattatctt tcgcaaaggg
    42841 ttaaccgttc aacaggcagt ttcagaatta gagttgattc aaaatgagtg tccagaaatt
    42901 attcctatga ttgatgcttt gaatcaatcc acaagaggca ttgtgaggta agcaatggtt
    42961 gttgcacctt acttggctgt agctatcgga ggctctttag gcgcaatgtc tcgttattta
    43021 gttacaataa tggctcaaaa tgcctggggg attaaatttc cttatggtac tcttttagtg
    43081 aacacactag gttcttttct agctggtttt tttcttattg tattagtagg tcgttttagc
    43141 gccgaggagt cgtttcgttt attccttttt acgggatttt taggagcttt tactaccttt
    43201 tcgagtttcg ctgcagaatc tttatttatg tttgagcaag gatattggtt taaattaatt
    43261 accaatatac tggtcaataa tgttggctca ttgtccatgg tcttcattgg aacattagtt
    43321 gctaaatatg tattattagg acatcagggc tccaattaat aatttataga gtgttggctt
    43381 atgttagata atcaattact tcgagagaat cctcaatatg tagcaaccca attactgaaa
    43441 cgaggatttc aatttgatgc ggtcactttc agtcagttgg aagaaaagcg taaggcattg
    43501 caagtaagta cgcaatcatt acagaacgaa cgaaatttgc gttcgaaagc catcggtgaa
    43561 gcaaaatctc gaggtgaaaa catagagcct atgcgcgaag aagtgaataa acttggcgct
    43621 aaactggagc aacaaaagac tgaactggac gaggttctta agcaaattga ggtaattagc
    43681 ctatcattac ctaatattcc tcatgagtca gtacccgttg gtaaagatga attggataat
    43741 caggaaatca gaaaatgggg cgacgtacct gctttttctt tccctgtaaa gtcacatgat
    43801 gaattgggtg aagcactagg gcaaatggat tttgcgcttg cagctaagat tacgggcagc
    43861 cgttttgtcg tcatgaaagg gcatctggcc cgattacatc gggctttaat tcaattcatg
    43921 ctggatatac atattcagca acatggttac caggaaatat atgtacctta tattgttaat
    43981 gcggatagct tactagggac tggacagtta ccaaagtttg aggctgattt atttaaatta
    44041 acaggagata atgggtacta cttgacgtct acttcagaaa ttccagtgac gaatacagta
    44101 agggaaatga tattgtctgc tgagcaatta cctattcgtt atgtttgcca ttccccgtgt
    44161 tttcgtagtg aggcgggttc ttacggcaaa gacacaaagg gaatgattag acagcatcaa
    44221 tttgagaaag ttgaactggt ctggattacc aaaccggaag attcttataa tgctttagag
    44281 caacttactc agcatgctga agttatttta cagcgtttga acttgcctta ccgagtggtt
    44341 gctttgtgta ccggggatat aggcgccggt tcagccaaaa cctatgatct tgaggtttgg
    44401 ttgcctagcc agaatactta tcgagaaata tcctcttgtt ccaatatgga ggcatttcag
    44461 gcacgccgaa tgaaagcgcg tttccgcaat ccagatacta atgagattca attagtccac
    44521 actctaaatg gatcaggttt agctgttgga agaactttag ttgccattat ggagaattat
    44581 caagatgagc atggaaacat tcatataccg gatgctttga agccttattt aggtggtatt
    44641 gatatcatct ctgtgaaata ggtttgctca taattatgga tattttttct taaaagttag
    44701 tcttcaatag gaaagagcga tagataggct atattgagcc aaatcaattg ctcttaagta
    44761 tttttaagaa aaaaatccag tgactctaaa ctaatccaaa tgaaacattt tgatttttcc
    44821 ttaagaatct ggccaaaaac cgtagatacc aattattctg aatcttaagt atcacatttt
    44881 agtctatggt tcatgaaaca ttctggagtc aagaatccaa tgaacgaggt aattattcgt
    44941 gagataacat ctgaagataa agagatgttt ctttgtgcta tgcaaagtag tcatgcatta
    45001 catcatcctt gggttaaggc accgataacg cctgaagagt ttgatgagta tttttctcgc
    45061 tatcaaaaac caaacccaaa aagctatctg cttctttctg acaataactt agcaggtgtc
    45121 tttaatatca gtgaaatagt acgtggttac tttcagaatg cgtatttggg cttttatgct
    45181 gttgcagctt atgcgggtaa aggatatatg agtactggtt tgaaattaat attaaccaaa
    45241 gtttttaagg acatagggtt gcatcgcttg gaggcgaata tacaacctga aaatacccgt
    45301 tcaatctggc ttgtaaagaa aaatggtttt cgctatgaag gattttcccc acgatatttg
    45361 agagtgaatg gcgtgtggca aggtcatgag cattgggcta tgacttatga agattttatc
    45421 aaggataata atgaagcgct tgaaaaagat catatcaata tagttgcata caatacggag
    45481 tggccccttc ttgccaaggc agaaatggtg aagttacgcg cctcatttcc cgcaaatagt
    45541 gttattgata tccagcatgt aggcagtaca gcaatacctg gcatggcatc aaaacccatt
    45601 attgatattc agattgcagt caggtcattg gaggaaatga agattattgc tgtgccgata
    45661 ttacagaaat tagcttatga atattgggaa gataatccag atcctgaacg aatgtttttt
    45721 gtaaaaggga tgccacccta tggtaatgga cgaacgcacc atgttcatat cgtcgaaaca
    45781 tcatccaggc attggaaagg aaaaatattt tttagagact atttactctc tcatccagaa
    45841 gcagcaaaaa aatatcagca actaaaaatc aaactggctc aggaacacaa atatgatagg
    45901 gagcaataca ctgatgcaaa aagtgaattt atcaactaaa ttttaaaatt agctcaaaat
    45961 taataaatta tcatgggcag acaaggataa atgggtgtaa cgaacttgga ttttttggcc
    46021 cagggttaga tctaaacgat gcatcaacgt ttttagaagt cctaatctta atgttgtgat
    46081 ctacaaacat tgtgatctat aattgtacta aatcacttta gttgagaaac gcaatggcag
    46141 atcaacctcc aaaatccaaa tggcatgaaa ttaaacgtct atcagaaggt gataataaag
    46201 tagataacaa tcaagcacca gtgtcaacgg cggatagaat taaaaagctt tatctggaca
    46261 cacaggtagg cagtccaatt gatactgttg aagaagcgga tgcccagcga aataatttag
    46321 ggcctaaaga tactaaaata gaaaattcct cccctgagaa tgatgagcca aaccctgata
    46381 taccaacccc cggatgatag cagaaattaa atacattgtt taaataaaat ggtttttgct
    46441 gtttaacgat accattctgg ttcggcaagc gcatgtgttg gttttgtgtt gacacccata
    46501 ataaatactt gtaaattttc ctacaagaaa atccttagtg ttcagtattc ttttagtcat
    46561 cctgattgaa cacgcgatta ggtttttggt gatggcttct ttatttttta ttaaagaggt
    46621 cataaccgtg tcagataaac cagattcccc atatatatta agcctgcaag cagacggtaa
    46681 tttatttcct agcattttac aatcaaattt agccatgaca ttggtttgga gttgttatgg
    46741 acaatttatt gaatgaaaaa aaattaacaa tttttacatc taatgaaatc caagataatt
    46801 tgaaccgggt tctgaaaaac tatgaacaaa atggcattat tggacttaat ggatatctca
    46861 atcaggcaaa ccgaataagt ttgcttaatg aacttgagat aatacaaaag gatgctgagc
    46921 aggatattgt caggtattgg aataataaaa tcattcattt ttattccaaa gatcctttga
    46981 aacctgtgtc agtggataga gaatatgtca cagaacctta ttttcaagct tcaagtaata
    47041 aggcacatgt attttatgaa gtgattgata atatgcgggt agtcaatcga attggccatg
    47101 gtatgcatct catcaatagg tatccgatga tgcagcgagc agtttatgat agtcctgtac
    47161 ttctggattt gctaaaaggg attggattta agaagccaat ctgccatctc agtgtctata
    47221 ttcccaaata tcctaatgga ataggcagtg aggtaaaacc tcatcaggag tcaacatttg
    47281 cctttactga accacaaagt gtcgttgttc tgtgggtcgc cctggaagac gcgttaattg
    47341 agaatgcctg tatgtatggg gttttaggaa gtaatcattg gccattgaaa tgggtatcaa
    47401 aagtggaccg agagacaaaa acgcgtcact ttgagcaagt tcatcagtta catatccctg
    47461 attttatgac agagcgcgaa ttttatactg ccttggaagt gaaagccgga gatgctttgt
    47521 tatttcacgg taattttgtg cactgcagcc caatgaatac gtctaaaaac tcaagaaaag
    47581 cattgtcgtt tcagtttatt gaaacgtatg aagtcgatta tccggaaact aattggttat
    47641 accctcctaa caaagtgtat ttatattaaa aattaaattt gataagcgat tgaggaatca
    47701 ctacatgtct atgtatgata agcgccagtt ttttaaaacc tatttttcta gcgcaagcga
    47761 gaagtcaatc ctggtaaagc tgttggataa acgatttcat cctccctttc acactgcgaa
    47821 catattggat ttgggttgcc atgatggcgc attgatgaaa aatatagtga atgcgtacgc
    47881 aaagcgttta ccggaaaagt tagttattac cggagtggat cctagtttgc aagcaattaa
    47941 agaatatgca aaagcagatt ttccaattcc agtccaaacg aatacttttg ctggaactgc
    48001 agaagattat tttgatgtgt gcactgacca ttttgattgg atttttgcat cgcagtgttt
    48061 gtattggtcc cctgatttgc caaaaataat taagcagatt catgaagcag gggactcggc
    48121 tttaattgtt atcagaggat gtcatggaat ttatgaaatt caatcgcaat tcaggcatta
    48181 tattgggaat catttagaga aattttatac tgataaagaa gttgagatgg ccttaaggac
    48241 ccaaaaaatt ttatttcaaa aagaatctct aagtactttt atccggttgc ctgataaaga
    48301 cagcattgaa ttgaaatggc tgattttgtt ttttttgcaa atggaagagg aatcgttggg
    48361 tgataaaata ttttgcgaag tgcgggattg gattacctct cgagcgtcag gaagcattca
    48421 tcatgaagtt catttttttt ggttagggaa tgccattttt acaggagatt acaataggaa
    48481 tttcccagtc aaatcgaaaa aaaattctag gggataaagg caatgttttc aatgaaattg
    48541 atttcatcat cagtcagcct gaactgatgg atggcaagat cttctttcat atgatccaaa
    48601 tttgtcgtgc cagttaaagg caacattcct atctgttgtg aaaatctaaa aatgatttgc
    48661 tcaatcgatt tctcgtactt tttgctgaga ttctcaagtt tcggatgcct taactcatat
    48721 tgattggccg ttaacagaga aaacccctga taaataattc catgggcctg gcaatagtta
    48781 cgaatttgtt tatcccataa tagcctggca aagcatctat tttgaacaaa cttgggtttt
    48841 accgaagcaa atgaaatgag ttcgattagc tgttcataac tcacattgga tatgcccagg
    48901 tgttttactt tcccatcttt atgcagggat tccattttgt tccagacttc tttatcttca
    48961 tcagaaaaat gatattgata ataagggcca tgcaaaatat aggaatttaa ataatcagta
    49021 tttaaatgtt caaggctgct tgcaaaagat tgctggactt gaatagtcag gcttgctgta
    49081 tcatcgtaag gtttgcaatg atcctggcca tgagcatagg tgaacttgga ttgcaaaaaa
    49141 atatcatcac gcaagatacc tgatttttta tagtattgtt gcaaggcata tccaacgcct
    49201 tcttcataat agtgttttct ttggtttgca gtatctatac cccgaaatcc ggtagttaat
    49261 gcaagctcga ccagttcgct tgtacgcttt tctttccagg cggttccata tataaaagtt
    49321 ggaaagccaa ggtccatcgt tcaatctcct tctcgactta ttatctgcaa acagactcta
    49381 ttttgactca aaaaacagag caaaccatag ctgactgtgt gtcttacata gggtttttcc
    49441 tgagatttct tgtcttacta tttgttcagg acttacgaaa aaccccaagg tcaaggcaaa
    49501 aaatcttttt aatgagggag tttagataaa ctaaatgacc gaattaaaaa cattttttaa
    49561 catagagatt ggggtttttc gtaagtcctg ttgttttcat tttcaatagt tacaaatcct
    49621 tacctaaaag acaacaggcg ggattaattc gaaaggttat tcgaagaatg tactaatttt
    49681 atggaattat acaaggaaat cagtcctgaa tcacaaagac cttggtattt tttaatgagc
    49741 tacagtcgca agcgactgta gcaggcaggt aagaattagt tgaattgata agcggttgta
    49801 ttctcttgct cagtctcagt cacttctggt ttgaggcaag cagaaaccca ggtagtacca
    49861 agacgtgtca taaaggcaat tccttcgacc agagcgttaa ttggtgccat cacagccaaa
    49921 gagagagcca aaccaaaatg tacttgtaag ttatagaaat tctcttttgc atgtaaaggc
    49981 ttacagatag ccaaattgac aatacactct atggcagaat caaccccata caacgcttcg
    50041 actaatgaat gaaatccact tttagccaag gatgttattg gggatgccat ttgttctaga
    50101 caatcccaag cattgttata agaagaaaag aacctggctt tattgaagta attggatgaa
    50161 ggcagctcat taaccatttg gtatccattt gctctgtttg cccactttgc cataagcccg
    50221 gtttctaata aatcatagcg ttgttcttgt ccaacaaacc atgatgatat aactctggtc
    50281 agaagttcaa ctgcatgagt cagagcgtga attggagcca taagtagtaa acaagtagag
    50341 agagtcaaat gacaacccac atctttgatc gcgtcgaatg ccaagcctgg ttttaaaatt
    50401 aataaattac ctacagtccg caaggttgcc catactgccc ttgaggcata atagccagca
    50461 tataatccat tgataatagg cgtaattcct actaatgcca aaccatccca gaatttgctt
    50521 gtagtggcat agtgattaat aaaacgcttg gctgaaacat attctctatc tggcaataat
    50581 ccttcatgaa tgagcgattc aatttctgtt ctaaggtctg tcattatggt ctctctctat
    50641 gttaattaaa caagcgggga gagtataact aaaaaaaaca taaatgacca atattggccc
    50701 atgttgtatt tgatgtttgc atctgttttc caaggtcttt tgaacacaga tcaaaggcca
    50761 tagaatcaat aatactctta ttgagtataa atcattgaat aaattttctt tattatggcg
    50821 tatgctccaa tcggccatga tgcagcacat actgtgatgg aaaatgcggt atgaaatact
    50881 acaaagatgg agtggggcat gaagtacaag gtaagtttta tcgaattaaa aacaataaat
    50941 agactttggg tagcaaattg tcacttattt ttttcaacca gttccagatg tttgtgtatt
    51001 aaattggtaa tgacatcaat cgaaaaatca gggcaacacc ggggatatgt cctgtttaaa
    51061 ccaccagaat gcttctctaa aagctgaagg tcatattcca acatatcttc tatatcgaaa
    51121 gtgtctttat tccatttgtc ttcattatga acaccgaaac ctccatcaaa aagcaaccca
    51181 ttaggtaatc ggattaagat atgccagcat tcatcagaat ttttcatcat aataaatgca
    51241 atatttactt tttcagtaaa tttttgattc caaatcttgt aaaaagcatt ggcaaatggg
    51301 ccacaagggc ctgagttaat tgcaggttcg ccaaaattgt ccccagcaat ggtgacgtaa
    51361 ccatagagtt tatttatttc tttagataag ctatttaggg tattaagaac attattttcg
    51421 gcattcataa acaattgtct catcttgggc atctggagtt gcagcactat caaatgcttt
    51481 aatcaaacgg acatttttaa aaccacaact tttaagaatt gctgttaact cctgcgggtc
    51541 gtaaattctt accctcaatt cttcaacctc tgtatgaatt atactattgt cgtgaacgag
    51601 ttcatattta ccgatagaat gacaaacctg atccttcagg gtcgctaatt gactgagcat
    51661 aatcatctgg ccattgggct tatgccaaac tgagcctctc cagacatcga gttgtggcac
    51721 tgctttgagt gtttcgccct caaacaataa aataccctca tcgcttaggt gattataaaa
    51781 tgtttgaagt gctagtctta ctgttgcagg atcgataagc aagcaaaagg aaccgcttgg
    51841 tataaaaatc aaattgtatt tttctggcct cgttaaattt tcaacaaaac ctctccatac
    51901 tgttggtttg agatttttag ctttggcttt aatattgagg gcttcaagca tatattcgct
    51961 tgcatcaaag ccatgtattt cgaatccttc ttcaagtaat ggaagtaaaa aacgaccggt
    52021 accacacatt ggctctaaaa tagggccttt cacatttatt acataactgc gataaaatgc
    52081 ataatcctct tcaggaggat tgggtttgct taagtcatag acctcagtgc acaagctaag
    52141 ataagtatcc aggtttttta attttgtcat cttgtttacc aattaaaaaa tttttgacta
    52201 tttgaatttg cacttcggta cttaggagac cgtcaatgac tatagtggca ttttgtttca
    52261 gttcatcatc aaaaaacaaa ggtcttgaat ggtttaaata aaaactgatt tcttcaagaa
    52321 gattttcttt agtttgtaat ttatctttaa aatccctcaa aatgcgacgg cacaatgaaa
    52381 tatcaagtgg cacctcaata tgaacacagg tatcaatata ttcacctgtt tgtacgtgaa
    52441 gtttccctaa gggagcatca aaaattatgt attccgcagg gttgagcaaa gtgcgtaaga
    52501 ctggatgcat aatttctctt tttgctttaa gctctgccaa aacctttgca aggtcttccc
    52561 tttgaaattc actgtaatta caacccttat gataccactc gatataatct tcgggttcta
    52621 aagaaatgtc atcaaagtca tcccatgaaa taagagttgc ttttaaatcc agggacaatg
    52681 cttttgccaa agtggatttt ccagcgcctg ttataccgct aatgccaata atatgaggag
    52741 tcataataat atctccatta cagtgactgg atgtccttta aatttatcca tgccgattat
    52801 ggcccaacca aggcgggagt aataattagg aatggttggg tcaaaggcaa aaagatgcaa
    52861 tttattaaaa cctaaatctc tggctttatt ttttgtggct tcaattagcc tgtttccaat
    52921 accttgtctc tgataggcag ggtcaaccac aagtgagcca agccatgggc ttatttctgg
    52981 cctgatacca tcattttcac gcaaagagca catcccaacg ggtttcccag tatcaagtgc
    53041 tataaacgta atgggtagtt tgtcagcatt taggtgctca ctgaaccgtt gaataacttg
    53101 ttcaaccggg atctcgggaa cccagatttt cccaagtatt tcatgccaaa ttgtcgcaca
    53161 ccttgctatg ctttgtggat gatttttgag aaggtcgatg gtgatcatgc tttacctgca
    53221 ttgacaagtt caggtattaa acttttgagg cgtagaatag tagttggtaa catttgtttg
    53281 agcacaagga tcttagcatg aaaagcaaaa gacttattga ggtctttaat tgctaaagta
    53341 atgtgattta ttccggtaat catttgatta aaccaatgcg tttaatcaat aaatgatgaa
    53401 gtttacccgc gccagagacg tcacctgaca tcaaaaaatc agcaacatac tcaaaacctg
    53461 cttttatata aacatgcttg gctctgggat tgtcacttgc gggatcaatt aaaaacgtat
    53521 cggctttcgg atcgacttct tttataaaaa aatcaataaa ttctatcaag gttctggccc
    53581 cataacctat gccaaaatag tttttatcac caatcatgta atcgatacca taagaatgtc
    53641 cagtcttgga aaggttaatc aatttaattt cgtcgatctc atcttcgctt gtttcctgga
    53701 ttgtcatcag cattgcgaaa ggatgcccat taccgcttgc tatccaataa acatatttac
    53761 cgtccgcata ggatgatggt tctgtgcggc cattgataaa gtttaagatg tcatctttat
    53821 gagcttgtgt gttgtcccag aactcctgaa caaagggttc tttaagccag ctaaagataa
    53881 gctcgatatg acttgcattg gctttttcaa aatttatgtt cattatgtgg aaaattctaa
    53941 tttttgattt tatatggtcg gagtgacagg aattgaacct gcgacccctg cctcccgaag
    54001 gcgatcccga ttgtttctta atgcatttct atacatctcc atgaacaata actccttgta
    54061 atacgagggt taatggcatt tttatgatgt acagtgatat attgttatgt tacattccac
    54121 aatgaaaatg caccctatat gcaccctgta attcaaaaat aggattttta ttacatgaat
    54181 gtgatattaa ctatgataaa gtccagggta ttaaaaaagc aaggattaag cagtagaaga
    54241 atttcacggg tgctataaac caaaaaacca ccgaaaaggt ggttcttggt caactgctaa
    54301 aaggggcgct ttcgcggatc cctaaaagct tacgttgtaa ttatgagtca ttttacatgg
    54361 atatgtcaat tttttttggt tattttttgg ataactattc taatttgatg caacaggata
    54421 gggtatgaaa gaatatatta gtgcattatc aatagatagg cttacaagct atgaaatatt
    54481 atgtccaagt aaagtggata cggatattat aggagcttat cattggaatt tattgataag
    54541 ccaagcattt tatcccttca tacattcagt agaagttgca ttacgtaatt caattcacca
    54601 cgcagccacc aagaaatttg aaaatgaatt ttggttcgat atcgttgtaa ctgatggtaa
    54661 gtcaagatca attcttgaag ataccaaaaa cgatttacat cgtagatttg ggcaagtaac
    54721 agcgtcagac attgttgcag gattaacatt tggattttgg acaaatttaa taaaacaaaa
    54781 aacatacact gatcaattta atccaaatcg actatggcct gacttaattc cttcggtttt
    54841 cccccattat gcaagagggc atgatgatag aaagaatatt tccaagcgat ttgaagaaat
    54901 aagactgatt agaaatcgct tgtttcatca cgagcctatc tggaaattta aaaatgccca
    54961 aacaccgcaa gaatgcattg ctgaattgcg tcgtaagttc aatgacacat ttaaagcgat
    55021 tggctggata tcaaaataca aacgaaattg tttaagagaa tttggttttg ttgaatcgtt
    55081 taaaaacaat tgcactcttg agaccttgga aagctataaa gtgaagggtg ctgaaatatt
    55141 acaacaatta gcagattaat tatgaaagca aaaatcaata acacgcttat taaaaaactg
    55201 cttcctagag ataaagaata cgaagttcat gatcttgatc taaaaggatt catgataaga
    55261 gtttttccat caggaaccat gcgttacgtc tgccagtaca aaagaggcaa aaaaattaat
    55321 cttggaacag tcggtgttat gacagcagct caagctcgtg aaaaggctat tgaagtgcta
    55381 agtaatgcca ataaggggat tgagccaacc gcacaaaaag ggataaataa gcctaagaca
    55441 cttcaagaat ttattgagag tgattataag ccatgggttt tatcaacgca taaacgaggt
    55501 caagaaacgc tagcagggct tattagatgt tataaggctt ttttttcaaa gccgttgggt
    55561 gaaattacgc ctgctcttgt agaacaatgg cgcgttaaac gtcttaatga aggcacttct
    55621 caggcaacct taaatcgtaa tatatccatg ctgaaatcac tgatgaccaa ggcagttgaa
    55681 tggggctttt taaaagaaaa tgcacttggt aagttaaaac cttataaaat cgatcgttca
    55741 cctaaaattc gatacctgtc atttgcagaa gaagcacgtt tacgcgaagc attaatcgaa
    55801 cgagacaatc aactccggct gatgcgaaaa aatggaaacc tctggcgaga aaagcgaggt
    55861 tatgatttat tacccgagct ttcagaagac aatgcttgtg attacttaat gccgatggtg
    55921 ctattgtcga ttaatacagg attaagacga ggcgaattat ttcatttgac ctgggatatg
    55981 gtgaatttgt cagaaggttc attaattttg tctggtgaaa ttacaaaaaa caaacattct
    56041 cgttatattc cgctcaatga tgaggcttat aaaattttgg ataatctgca taaaaaagca
    56101 gagagaaaag aaggcttagt ttttccaagt aaaaataatc aaccatttaa ccatgtgaaa
    56161 cgctcatggg gatcgttatt gaaaaaagcg aatattacgc agtttcgatg gcatgattta
    56221 cgacatcatt ttgcgagtaa gttggtgatg gctggggtag atctaaatac cgttcgggag
    56281 ttattggggc attctgatat tgccatgacg ctgaggtatg cgcatttggc gccggagcat
    56341 aaagtgaatg ccgttagaaa aatagattgg tcaatgcagg gataggaaaa tgaatagtca
    56401 tacttcaaaa tatgcagaaa gaaacaggcc cataagaaga attgagcgat ttattctcaa
    56461 gataatgatt tttgttgtca tacccattag ctgcatattg ggaatagttt atccaactag
    56521 caaaataagt aattctttac cggtattatt ttcattagct ggtttgatcc aactcgaagt
    56581 agcaggcttg tttgagtata tagattatct gggtaatgaa ttagaaaaaa tatatgacaa
    56641 atcgggtcgt gttcctagta acattacaag agcaatctat gaatattata atcctgagcc
    56701 aactgtaaaa gcatttatta ataaccaact ttacttaaac aaacgagttg gcttctatct
    56761 attgttgatt ggcggattga ttcaattatt cactatctgg cttgaatagc cattccttct
    56821 gtatcaatcg aaattagtaa tccacttgtg tcagaatata cagagagtaa ctagttgatt
    56881 tttaggggta gattgggttg atcgtatgcc ctagtttgat aaggcatcaa atttccatcg
    56941 atgccaaata actggggcaa tagataaaat tgaatctgct gacataacgg gatcactgac
    57001 tgaagttcta ttgttattac aaaccaataa atacttatct tcacttttta tcaatcgtcg
    57061 aaaaaaggtg ccttcggtgg tttccactat acaattgtgc cctacgcatt gatttttttt
    57121 attttgattt ataaatttaa tcccgccaac aatgtctcca actttatact ccggctccat
    57181 cgcatcatct gagaccgtta ttacaacagc atttgggtta tttgttttaa agaagtctat
    57241 ctctttttgt atggcttgtt ctgaggcgag aagattacca actgtatctg caagaaatgg
    57301 ataattttga aattcatttt cgattactga tgggcctttt cctatgcctt ctaataacca
    57361 atcaattgaa caactcacat catattgatt aagtattcct actaaagaag cagctttggc
    57421 tttatttaat gggtttcttc caagctccca tgattggagc gtatggatac tgattccatg
    57481 cttttcttcc aaatcttttc gagataagcc agcaagcata cgtgctcttt tgatgcgttg
    57541 tcccattgca gaactatctt tttttacttt agtagcattt ttcataaata tgtctttaca
    57601 tttatacgct ttatatgtat ataataaatt attatacgcg atacacgtat atgaagtgtg
    57661 gatttatacg cgttatgagt gtaaggatgc ctctaaaatt aattattttc aacactttaa
    57721 agaggattct gttttgaagg tcaatttcag ataggaatgt aagtagtcaa tggacatgtt
    57781 aataaaccag gatgaactgg cagcaatgag tggcttacct catatccagc aattggctta
    57841 tctaagagga atcagaccgt atatggatgt taaaacagga ataacaggta tcaagcgacg
    57901 aatcagctat caatcgatat ctgaacaatt atatatcgag ccacatcagg ggattaaaag
    57961 ccaaagcttt tctcgtgatc aagtacgtcg tgcagtttca ggtttggtac gtgcgggaat
    58021 gattgaagtt caatctgacg gcatgcagct cattttaaaa tgtcccttag cttcaaggca
    58081 ttattctgcc caaaataaag ccgccataaa cccgccacag aaagccacca taaagccgca
    58141 cgtggaaacc cttgtaaaca atgagttttc tgaaggtgag gccataaaag ccgacatagc
    58201 tgaaccccaa aaagccgcca tacctcttaa agataataat tatatttatt tattaagccg
    58261 cttcgaacaa ttttggaatt tataccccga gaaaaaatcc cacgagcgtg cttttgaagc
    58321 cttcagaaaa atcagccctg acgagcatct attgcgaatg atgttgcaag cactggaaaa
    58381 ccaaatcaaa gcacgaaatg taaaaatagc ccacggcgaa tgggtgccac cctggaaata
    58441 tccaagcaac tggttggttc aaaaatgctg ggaagatgaa gtcaaaatcg aagtaacgca
    58501 ggagaaaagg aatgcaaagc gccacccaaa tactgaactc acaaccgttg atccattctg
    58561 gaacccagaa ggagaagaca cagcaagcac cagtgaagac gagtacgaac aaagcaatgt
    58621 catcaacctg cgatgctacc gacagcaata aaaagcggat tgagagtttg tttacgcgct
    58681 ttgcagtttt ttatgggcat ttgtggcgca gtcagttcaa aagcgatggg tttttggagt
    58741 ttgccaaaaa ggaatggatg gcggggttaa gcaagtttag tgatgacgtt ttgaatcaag
    58801 ccatcgtgga gtgtcgtgat ttttgtgaaa tgccgccaag cttgcctcaa ctgattcgca
    58861 tttgtcgtga cataaaaaaa cgaaataacg tttatgtcac gcctgaagaa gttgctcctg
    58921 cgagtgccga agtggcggaa gcgaatatta agcaatgtaa agcattttta ttttaaaacc
    58981 tgataggaga gaagacatgt tggtattaac aagaagagtt ggggagtctg ttgtgatcag
    59041 tgaggatatt tactgcacga ttgtggggta tcaaaacgat gaggtccgcc ttgcttttga
    59101 tgcgcctaag tccatcccaa tccatcgtga tgaaattcaa cgccgcattt accgcgatca
    59161 gattaaagat aacaagtttg tagataaagc agctaacaac gagagcattg tcgacaggct
    59221 tattaataag ttcaaaagca gtgcttctcc aacaaattcc tgagaattga gtcatgacta
    59281 tcgatgaaga acagctgatt acggttaaag atcgagccaa agaaaagtta aggcgtgatc
    59341 tgggtggatt gattgaacgg gctttaaacg atccaaaaac cgttgaaatc atgctcaatg
    59401 cggatggcag gctttggcag gaacgtcttg gggaaacaat gcgttgcatt ggaagtatca
    59461 atgaggtgcg tgccgaaagc atcatcaaaa ctgttgcagg ctttcatggt aaagaagtga
    59521 cccgatttaa gccgatgctt gaaggggaat taccactgga tggttcccgt tttgcaggac
    59581 aactgccacc ggttgtatca cgccctacat tcgctattcg taaaaaagcc ctatccgttt
    59641 ttacactgga tgaatatgtt cgagcaggaa ttatgacgga tgagcaaaac gagataattc
    59701 aacaagcggt ttcgaaccac cggaacattt tagtgattgg cggcacaggt tcaggcaaaa
    59761 caacattagt gaacgccatt attcatgaaa tggtaaaaag cgcaccacat gagcgcattt
    59821 ttatcataga ggataccggc gaaatccagt gttcagctga aaactgcgtt caataccata
    59881 ccaccatgga tgtcaacatg acccaactta taaaaaccac actcagaatg cgccctgacc
    59941 gaatccttgt cggtgaggtt cgaggcagtg aagctttaga tttattggat gcctggaata
    60001 ccggccacga aggaggagcg gctaccttac atgcgaataa ctgtcttgca gggcttcatc
    60061 gtttgaaatc attgattacc agaaatccgg cagcacccgc tgaaattgaa cctttaattg
    60121 gcgaggcagt tcattgtgtg gtgcatattg caagaacgcc agatggtcgc tttgtcaaag
    60181 aaatcatatc tgtcgagggt tttaaaaatg gtcaatacaa catcaagaaa cttgtttaag
    60241 gagaatatga atgaatcagt taagcagttt taatcacaag aaaatttgga tgatgggggc
    60301 agtcatatta tttctatttt taatgactca tccggctagc gcctccacag caggtggagg
    60361 acttccattc gactcatggt taaccaaaat ccagaagtcc attacaggtc catttgcctt
    60421 tagtgcggcg attattggtc tggttgctgc cggtgccaca cttatttttg gtggtgaatt
    60481 aaatggtttt atgcgaaccc ttgtcttttt tgtattggtg ctttcattcc tggtagcggc
    60541 acaaaatacc atgacagcga ttactggcaa aggggcggaa atttccaagc catctgttga
    60601 gatgacagca atcgagtctc agccatgagc cttcgcacta tctccattcg tagatgtgga
    60661 aatcgtccaa gtctctttat gggcggagac cgtgagcttg tcatgttttc aggattgcta
    60721 tcagccattt tggtatttgc cgctcaggat tggctggcgg cgattgctgg tatcgtcatg
    60781 tggtttttat ctctgaaagg acttcgctta atggcgaaat ctgatcccta tatgagagcg
    60841 gtttatctga ggcaaagacg ttatcaggct tattatccgg ctcgttcaac ccctttcagg
    60901 gaaaacaaaa gaggttatca atgattgaat taatcgcctt tttgattagt actacagggc
    60961 ttgtcatttt gggtactttg ttttgggaaa ttcgaagtaa aagctgtggg caccatgtca
    61021 aaaagcatcg aagccataca gctggactgg ttgatttact taattatgcg gcggttgtag
    61081 atgatggtgt aattgtcgga aaaaacggct cttttatggc ggcctggctt tatcatggcg
    61141 aagacaatgc caatactacc gatgaggcgc gtgagatggt gtcatttcgg atcaatcaag
    61201 ccttatccgc tatgggtagc ggttggatga ttcatgtaga tgccatgagg cgtactgcac
    61261 ctggttacag tgcacggggt tattctcact ttcctgatcc catatcaatg gcagttgatg
    61321 aagaaagaag gcagctattt gaacagttgg gaaccttata tgaaggatat tttgttatca
    61381 cgttaacctg gtatccgcct gtattggtac aaaaacgttt tgttgaattg atgtttgatg
    61441 attcagggga gcgcttaagc cagaaagcaa aaacgcatca attgattgag accttcaagc
    61501 aatcttgccg caattttgaa tcacgcatga gttcggcagt tcatctggaa aggcttggct
    61561 cagatgataa aggcgataca gtaatgcagg ataactttct aaggcactta cagtattgtg
    61621 tcacaggaat taaccatccc gtcaatctgc ctaaaaaccc gatttatctt gatgccttga
    61681 taggcggtca ggaattaacg ccaggcatta ctccgaaaat tggtcggaag tttgttcagt
    61741 gcgtcgcgat tgaaggtttt cccatggaat cttaccctgg aattttaacc gcgctatcgc
    61801 aactacccat tgaatatcgc tggaactccc gcttcatatt catggatgcg catgaagctg
    61861 tcgcacattt cactaaattc cgtaaaaaat ggaagcaaaa agtcagagga ttttttgatc
    61921 agatttttaa taccaattca ggcattgtcg acgaagatgc gcttagcatg gtcaatgatg
    61981 cgcaatcagc cattgctgaa accaattctg gcatggtagg gcaaggttat tacacttcgg
    62041 ttgttgtttt aatggatgaa gatcgcgcac aagttgaaaa agcagcgctg tttattgaaa
    62101 agaacatcaa tgcgttgggt tttgcggcca gagtagaaac cattaatacc atggatgctt
    62161 ttatgggcag cctgccaggc catggcgttg aaaatatcag acgaccattg attaacacca
    62221 tgaatctggc ggacttattg ccaacatcca gcatctggac aggtgagaat aaagcaccgt
    62281 gcccactgtt cccacccttg tcaccacctc tgatgcattg cgtcaccagt ggtaatgcac
    62341 cgtttcgcct gaatcttcat gtcagagact taggccatgg cattatgttt ggtccaacgc
    62401 gttctggtaa atcaactcac cttggtttac tcgcattgtc ttggcgtcgg tataaagatg
    62461 cccgtattta ttcatttgac aaaggactat ccatgtatcc aacctgcaag gcgacaggtg
    62521 gtgagcatta caccattgct gataaagact caaagcttgc ctttgcgccc ttgcagtttt
    62581 tagagtccaa ggcagacagg gcttgggcta tggagtggat tgatacaata ctggcattaa
    62641 atggtttgga taccacccca gcacagcgta atgaaatcgg ctatgccatt atcagcatgc
    62701 atcaaagtgg ctctaaaacc ttatccgaat ttgtcatgac cattcaggac gaacacattc
    62761 gtgaaaccct aaagcaatac accatcgatg gattgatggg gcatttattg gatgctgaat
    62821 gtgatggttt aggtctgtcc tcgtttatga cctttgaaat tgagcactta atgggattag
    62881 gcgaaaaatt tgcactgcct gtgttgctct atctgtttcg acgcattgaa acctcactgg
    62941 atggccgccc aacgctcatc ttactggatg aagcgtggtt gatgctcgcg catcctgtct
    63001 ttaaaaacaa gattgctgaa tggcttgatt ccatggccaa gaaaaactgc gctgtattta
    63061 tggcaactca acatctttcc catgcagcaa aatccggcat tttagacatc attgttgaat
    63121 ccacagccac caaaatcttt ttacccaatc tatatgcaag agatcctgaa acctgcgtga
    63181 tttatgaacg catgggctta aatccacgtc agattgacat catagctcaa gcccaaccca
    63241 agcgtgatta ctactatgtc agcgaaaaag gacaacgtct ttatcaatta gcattaggct
    63301 ctatggcttt ggcttttgta ggggctactg atcccgattc tatagctcgg atgaaacagc
    63361 ttgagttgaa gtacagtgat ggatgggtat ctcagtggct tacggaaaaa ggaatccaat
    63421 tggaacagta cggagaagca gcatgaaaaa aataaaacaa tggcttaata ccaaaagacc
    63481 tgaaaagaaa aatgaagcgt taacggagaa tccctttctt aatgccagac gcgcctggaa
    63541 tgttcatacc gcagggctta tgaaatcggt acaggtttgg caactggtgg gcttaagcag
    63601 ccttttattg gcactggccg ctgttggtgg gcttatatcc attggcagcc agtccaaatt
    63661 tatccctttg gtatttcagc aggacgctaa tggcaatacc ttgtcagtca cccgtgcgga
    63721 tagggttgga caagccaata ttgatgatta tcgggcggct gctgcacatt ttattgaaaa
    63781 tatccgcatg gtaagtagcg atgttgaact acaaaagaag gctgtgtttc aagtttattc
    63841 ctatttaaat cagaatgatg cggctctagc caaagttcag gaattttaca gtaataaaca
    63901 gcagtctaat ccctttgaaa gagcgacgca tgaaatcgtc agcattgaga ttcgatcagt
    63961 tctacaagag tctgatgaga cctggcaggt tgattgggtt gagacggtga gaaatcgtga
    64021 cggaacgctt aaagaaaagc cctatgtgat gaaagccatg atcacgatgt atcaggataa
    64081 cgaacttaat gatgtctcca gtgagtctat tttgaaaaac ccacacttaa tttttatccg
    64141 tgattttaac tggtcctatg aattaaagca tggagaacag cgatgaaaaa aataatgctc
    64201 actttttgtt tattgatgcc attgacaggt tttgccctgg ataataatga gctggccact
    64261 cgttattttt ccgaaaacga tccaaaactg tcaaatcagg aaaagcaggc tttagatatt
    64321 gcagagcgtt ggcaaaaggg agacaaaacc agcaaaccat ttccatcctc ggatggatca
    64381 gtcagttttg tttatggctc agggcaaatc agggtagtat gcgctccctt gcaggtttgt
    64441 gatattgctt tgcaggcagg tgagcagttt aacgatatga atgttggtga tccacgcttc
    64501 attgttgagc catccattac gggttcagga gctgcgcaac aaatccactt attgatcaag
    64561 cctaaagatg ttggacttga ttcctctttg gtcgtaacta cagacagaag aacttatcat
    64621 ttcaggttaa agtccgaccg cactgagttt atgccttacg tatctttcat ttatcccgat
    64681 gaagcaaaag caaagtggca gttcattcaa aatgttcagg ccgaaagaag aaaaatggat
    64741 acgttaccag aaaccaacga ataccttggc aacctgaatt tcaactaccg aattcagggc
    64801 catgcacgat ttaagccgct tcgggtttac aacaatggcg ttaagaccat tattgagatg
    64861 ccgaaaacga tggcacaaag tgaagcccct gttttattgg ttttaagaag ccgtggtttg
    64921 tttcaaaaat cagagtccgt catggtgaat tatcgtctgc aaggttgtcg ttacatcgtc
    64981 gatagcgttt ttgatagggc aatattggtt atcggaagtg gaagctctca agaaaaaatt
    65041 accattacga ggtgctaaag atgaaggaac taagtgtttt attgtttgct ctattgctat
    65101 caagctgtgc aagtcagcgt tatgggaatt ttactcaagt atctcaaggc cgagacgttt
    65161 atttggcaca agacgcggca tcccaattaa cacgtgtcta tccacctgct caaaatacgt
    65221 tttgcatcag tcaaaaggta agggatgctt ttggcatcag cctgattcat gaaatgcgaa
    65281 aaaaaggtta tggcgtgatt gaaaacaatt gccccaaaca aaaggccaat tttttctatg
    65341 ttgtcgatga gattaaagca accaaccttt accgagtcag cctttttatt ggctcacaga
    65401 ccttcagcag aatttatgcc aaaaccggtg gaaaaattcc cccagttagc gcatggactc
    65461 ataaggagtg agactgatga atcaaaacaa cgatttttta tcaaaagact actcaccgca
    65521 aaaactccag acagccggtg tgaagcgggt gaataacgta ccattaatca ttgtcattgg
    65581 tattttaact gtgtttgtgg tgttgatagc ttttgttgcc aataaacggg caaatgcaca
    65641 gaaccaatct cccgaaatag taaagattaa aagtcagaaa aaaaacacca tgagtctggc
    65701 ttatgaagtg gtaggaaacc acaaagtacc agtcgtagct ccgcaaactg aaacgatcac
    65761 aatcaagcag gaggcttccc tacccatact tgagcaacaa gacttgcctc aaaaacaaga
    65821 gacgttggat caaaatgtga ctgatgctga aatagaacgc attcgccagg aaaaaacaca
    65881 agaatttgaa gaggcggtca aagcaaaaac atccattatg gtggataacg tacggctgaa
    65941 taacagagca agtgtaagga catccatgag cgggaatgac gtgacattca atgttgaccc
    66001 atctacggcc tttaaagagc aactcatgtt ggtacaaggg atgcacaaca aacccatgcc
    66061 gcaaacctta ggtggtgaag aaaatgaaat gcgttggcat ttaaactcaa gacttgaaag
    66121 tccaaacagc cgatatgaac tcagagcggg tggggtgatt ccaggagtaa tggtcagtgg
    66181 catctcctct gaattaccag ggcaaatcat cggtcaggta tcacaaaacg tctatgacac
    66241 cgcaacgggt aaataccttc tcattcctca aggaaccaaa gtatttggca tttattccag
    66301 tgatgtgagc tttggccaga attccgtatt agtggcttgg cagagactgg tatttcctga
    66361 tggcaaggca ttggatattg gctccatgcc aggcgctgat agtgcgggat tcgccggatt
    66421 tcgtgatcaa gtggatcatc attatgcaag aatttatggc tcagcgcttt taatgtctgg
    66481 cattgttgca ggtattacct acagccagaa tagtaatcag cctaaccagt tcggttacag
    66541 ccaacccaca gcgggttctg tcttgagtca ggcattagga caacagttag gtgaggtgac
    66601 atcacaactg gtttcaaaaa atttgaacgt atccccgacc attaatgtcc gtccgggtta
    66661 ccgtttcaac atcattgtag tgaaagattt aacttttaaa caactttata gacagtttgc
    66721 ttattgatac aggaggagtg caatgaaatt tagaatcatg cttatttatt tatggacact
    66781 acatgttttc gccagcggtg cgcccgtatt tgattttgcc aattggattg aaaatggaaa
    66841 aatcatactg aatcaggtga gtcagtataa aactcagatc gatcaataca acaatcagct
    66901 gcagcaatac caaaatatgt tagagaacac taagtcgctc acttccttcc agtgggataa
    66961 tgccaactct gtcattaata atctgcttga atcgactaat acaattgatt attacaagca
    67021 ggaagcaggc agccttcaag gttatctgga ccgatttcag agccaggaat actatcagaa
    67081 aactccttgt tttaatggta atggtcattg ctcacccgaa gaactcagga aaatcaaaca
    67141 aagtagactc tcaacttctg ttgccgaaaa gcgtgctaat gatgccatgc tcaaagggat
    67201 tgataagcaa caacagagct tgaaagatga ctcagaaaaa ctacgggtct tgcaatctca
    67261 ggcgcaaagt gccgttgggc aaaagcaagc actacaggct gcttcacaat tagccagcaa
    67321 tcaatcacat caattacttc aaatcagagg gcttttatta gcacaacaaa acgcgcaggc
    67381 ggtaaaagat gcggcaaacg ccaataaaga ggccatacag gccgcgggtg atgagcaatt
    67441 tcgagcgggt acgtatcata aaagttcagg taaaaaatgg taattcagtc ataacgacaa
    67501 ggagaagaaa tgaaacgctt aagtttatcc atcatggtgt tcgcttgttt gctttcaggc
    67561 tgtgaaagta cagaaaccaa agcaaggaaa aagtcagagc acttggcctc gttaacctgt
    67621 gacagcccaa gagaaggcca cacaaaagaa gaactccagg ccattgtcga ggcttgtttc
    67681 agagggggaa gctattcaaa aagttcaggg aaaaagtggt aatactatga ataaaaagac
    67741 aaccacctct ttactcatac tctgtttgct aggcttttct gtatgctgtc atgcccaggg
    67801 ccatggtata gacagcagag atttgctaga caatatctta tatcgttttt ccagcactgc
    67861 ctcgatgtgg agccaaacta ttttaagtta tgctcgttac cttttttggt ctttggccac
    67921 catcagcatg gtatggactt atggtctcat ggctttgcga aaagcagaca ttcaagagtt
    67981 tttggcagaa accgtaaaat ttttagtggt tgtcggtttt ttctattgga ttttagataa
    68041 tgggcctgcg attgcaactg caatcatgga ttctatgcga ctgctcgctg caaaagcttc
    68101 tggaattaac gaacatgtgt cgccttctga cattgtggat gtggggtttg atattgtatc
    68161 aaaagcaatc gacagctcat caatctggtc accggctgca acgacggtag gattaattgt
    68221 tgctgggctt attctgatta tattggcgtt ggtcagtatt aatatgctca ttatcctgat
    68281 tacagcctgg atcttgactt atggtggcat cattttatta ggttttggcg gcggacgctg
    68341 gacacaggat attgcgatta actattacaa aacagtacta gggatcgcat tacaagcctt
    68401 tgccatgatt ttgattatcg gcataggaaa gtcttttgta gatcaatatt atgcggcaat
    68461 gtcagaaaac atcatgctaa aagaaatgtt tgtcatgatg gttgttgccg tacttttgtt
    68521 ggtacttatc aataaaattc cccctgtttt atccggcatc gtttcaggtg gaggatcagg
    68581 tggcggtggt ggacttggtc ttggaggcgc gttaggggct gcgggaattg caggaacagc
    68641 cttagcaggc gctgcgggtg ctgcagctgt gcagagtgct gggggattat ccgctttaca
    68701 ggctgcattc aaggctgctt ctcatagtat cggcgcaagt ggcgctggct ccgtgtcagg
    68761 agatagccca ggctcaaaac aaggtagttt ggctcaggcc atgggacaag cttctaaatt
    68821 tgctggttcc tttggttcac atttagcttc tggggcgttt gatgtcgccc gtgaaaaagc
    68881 agcaagcatg aaagatgctt tttctgcaaa agcggctgat accacaggcg gtaaaattgc
    68941 tgatgccatt catcagcgca tatcttccaa tgctgaatcg ccaactatta atcaacaggt
    69001 atccgagtct caaaatgtca cttctatggg tgctccagaa ggcagtttta gttcatgtaa
    69061 cataaacagt gatgatgaga tcagtcaatt tgtcaatcaa aaagcatctg gagacggtca
    69121 atgagcttga ataaacccaa caaagccatt ggcccacagg tgaggcaaaa gcataatgaa
    69181 aaaacgaata aacaacttgt tattttagtt gccctctcgc ttttcatcag tatgcaggtt
    69241 ggcacacaat ttgtggctta taagttgcat tttagtgatg aattagggtt ttcgctgaat
    69301 catgtctact ttccatggca gtctctttgg tggagcttga aataccatca ttattatcct
    69361 gattatttta atgcagcctt tggtttgatg gcgatgagtg gcagtctttt tttaatgttg
    69421 attgtttttg ttgctaagca tttaaaaaaa gaaaacatca gtgaatacct gcatggttca
    69481 gccagatggg ccaacagaga tgatttaaaa aatgctggac tgtttggcaa tgaggaaggc
    69541 gtttatgtcg gtgctattga agatgaaaaa ggggatattc attatttacg ccataatggc
    69601 ccagagcata ttttaactta tgccccaacg cgttctggca aaggggttgg tcttgttatt
    69661 cctacccttt tatcctggaa acagtcttgt gtgattacgg atttaaaagg agagctttgg
    69721 gcattaacag ctggttggcg tcaaaagcat gccaacaata aagtcattcg ctttgagccg
    69781 gcaacattaa agggttcagc ccgatggaat ccattagatg aaattagagc aggtacggaa
    69841 tatgaagttg gtgatgtaca aaatcttgca acgcttgtaa ttgatcctga tggtaaagga
    69901 cttgaaaccc actggcagaa aacatcccaa gcacttttgg ttgggtttat tctacatgca
    69961 atttataagc tgaaaaatca aggagaaccg gctacttttc caaatattga tcgcatgttg
    70021 gtcgatccaa ataccaacat cgccgattta ttgattgaaa tgactcaata cccacatgtt
    70081 gatggcaaaa cacattttgt tatcagcgca tcggctcgcg atatgattga taggccggag
    70141 gaagaagcag gttctgtctt gtccacctta aaatcgtacc tggctttata ccgtgatccg
    70201 atagttgctt acaatgtctc tgcatcagat ttctgtatta aagatttgat gcatcatgca
    70261 agccctgtca gtctttatat cgtgacccaa cccaatgata aagcacgatt acagccttta
    70321 gttcgggtta tgctgaatat ggttgtccgc cttttggcgg ataaaatgga atttgaacga
    70381 gtcgatgatg ggcgagggaa ttatttcgta agaacgaaga aaacctataa acatcgcctg
    70441 ttgtgcatga ttgatgaatt cccaagtctt gggaagcttg atattttaca agagtcattg
    70501 gcgtttgttg ccggttatgg tttgaagttt tatttaatct gccaggacat taatcaactt
    70561 aaaagtcgtg agcgcggcta tggcccagat gaaaccatca cctcgaactg tcatatccaa
    70621 aatgcctatc cgcccaaccg aattgaaacc gccgagcatc tttcaaaact aaccggccag
    70681 actactattg taaaagagca taccactact agcggtaaac ggttttcaac ctttctaaat
    70741 cagatttcaa aaacaaaaca agaagtttct cgaccgcttt tgacggttga tgaatgtcaa
    70801 cgtatgcctg gaccaaaaaa agatgaaaac ggtttaatca aggaagcagg cgatatggtg
    70861 gtgtatgtgg caggatttcc tgccatttat ggcaaacagc cgctttattt taaagaccct
    70921 gtgtttatcg ccagagcttc tgttgaagcg ccagagcaat cagatatctt acgcgcccgc
    70981 cttcgtgaag atgaggaaat cagactatga aaaagttcac ttcctggatt gctgttctta
    71041 tgttaagcat catcggcatg gcatttcttt tcatgctgat gggctttcgc gtgaacacta
    71101 cagattccat tccttttggc ctttatcgca ttacaaatat aaaaaacatc aaaaatgcct
    71161 atgtgatttt ttgccctgat gatcgaccgg tttttaaaca gggattagat agaggctata
    71221 tcggaagtgg cctttgccct ggcggttatg ggtatttaat gaaaaaagtg gtggcaacaa
    71281 atggtgatca aatatctatc acctccgacg gcgtttttgt taatcgccaa cttattctct
    71341 tttcaaaacc gacattaata gatggaagga aacgaccttt gcctcaatgg cgcaccatag
    71401 attatcagat caaagaagat gaactcataa ccatgactag ccaaagcgaa tgctcttttg
    71461 atagtcgtta ttatggccct attcggatag ggcaaattaa gggagtaatt aaacccatat
    71521 gggtaaatgc aacaagtgga gaaatagcat gaataaagaa gctgaattaa tggatgtaat
    71581 ttcagaaaaa ttagatgatt tgatggttcc gggttttatc gccgaagtca ctcccatcga
    71641 ggcagaaatc atgggtgcct tttctgagga tgcgcttagt gaagatgacg ctaaggaggc
    71701 ggcctatgac taaattgtct caccatcagg ttgtggccaa ccaaatcatt gaaagcctca
    71761 aacaaggaac cgcaccctgg gtaaaaccat gggagccaag cacgggcggc ggccaaatcc
    71821 cgtttaatcc agtcacagga aagcggtatc gcggcattaa tgccttgtat ttgatgttaa
    71881 atcaaagcgg ggataaccgc tggctcacct acaaacaggc gcaaagcatt gacgcgcagg
    71941 ttagaaaagg agaaaaaggc acgactgttc aatactggaa atttactgat gaaaaaatca
    72001 aaaacgatga tgcgggaaac ccagtcctcg atgagcaggg aaatcctgtc aaagtgcagg
    72061 ttaaccttga aagacccaag gtgttttacg caacggtatt tcatgcctca caaattgata
    72121 atatgccgga acttgtccaa aaagagcagg actggtcttt aattgaaaga gcagaaaacc
    72181 tgctacttaa ttctggtgct gccatttttc attcggaggc tgatagagca ttttacaggg
    72241 tatcaaccga cagcattcac ctccctccca aagagcaatt taaatcagcc gcccattatt
    72301 acgtcacagc ccttcatgcg cttggtcatt ggagcgggca tccttcaagg cttgatcgag
    72361 acctacgcca tccctttggc agtgaagctt acgccaaaga agaactaagg gctgaaatcg
    72421 ccagtatgct gttgggttcg gagcttggta ttggacatga cccctcccaa cacacggctt
    72481 acatcaaatc atggattcgc gttttagaag gtgatccgct ggaaatattc agagcgtcag
    72541 cggatgctga aaaaattgtc aatcacattt gctcattgga acaagcacaa gacatccagc
    72601 atgagcaatc tattgtaaac agggatgaaa aaaataatga ggaaactcta atggaatccg
    72661 aaaagaatat ccctgaaaaa gtctggctta gcatcccatt caaacaaaaa gagttagcca
    72721 aatccgtggt agggaagttg cccgatggaa cacctggcat tgcctgggat aaaacacaga
    72781 aatgctggta tgcaaaacca ggtgtttcca ttgaaaaaat caaagcctgg cttccagaaa
    72841 atcaaaactc accagaagac aaagcacttt caccagccga tgaattcaag gaagcgttaa
    72901 tcagtttggg tgcagtattg acaggtgagc atcctatcat ggacggcaag actcatcgta
    72961 tacaaatgga ggacgataaa actggtgaaa aggcaggatt ttacgtggcc catttagatg
    73021 gtattcccgc aggctatatc aaaaataacc gcacaggtgc tgagctaaaa tggaaaagta
    73081 aaggttatgt tttaaccgat gaacaaaaag cagcacttaa ggctcaggta ctcgaaaatc
    73141 aaaaaaatcg cgagcttgaa ttggtggaaa ggcacaaaag caccgcatta aggctcaagc
    73201 aaaggctttc caaaatgagc gagatcaccg agccaacacc ctatatgaaa tcaaaaggca
    73261 ttcaggttca tccagatgtt tatactgatg atgacaaaaa attaacgtgt attcctgcaa
    73321 ctgatattga aggtacaatt tggacggttc aatatattgc agaagatggc accaagcgct
    73381 tcgctaaaga ttcaaaaaag gaaggctgct ttcatgttct gggcggcatg aacaaacttt
    73441 ttgatgcgcc ggttattgtc attgcggaag gatatgcaac cgctgcaacg ataaaagaag
    73501 catgcgggct gcctgctgtc gtttctgcct ttgattcagg taaccttaaa tcggtggcga
    73561 aagtattgca tgaaaaatat ccatatactc ccgttatgat cgcatctgac gatgataagc
    73621 acctggagtt aacgcaaggc atcaatccgg gtaaagaaaa agctttggag gctgcgggtg
    73681 ctgttaatgg ctttattgtt atgccgactt tcgcgcccca tgaacaatct ttaaacccta
    73741 aaaaattcag cgattttaat gacttggcaa atcacagtat gctgggcttg gatggggtta
    73801 agcgtcagct caaaccaaaa attgaaaaag ttgccaaacg atgcaattgt ctgcgcttat
    73861 tgcaaaaaag taatgggatt catattgctt aggttatttg ataaggtgaa gtttcttact
    73921 ttcatcaatt aaccagcgaa ggccatcagt aatcttggtt tgtaataaaa tatgggcgct
    73981 ttcattaaag aatcgcggca agccttcata acaacccgtt attgttcctt ccttatcatg
    74041 ccagaaaaaa gtatgtatca attctttatg ctttgacatg gattcagtga ttgcaccaaa
    74101 tgatacgagt tctttgagct ttacatcacc gcctaactct tcctcaaggt cacggattac
    74161 agcttgttct ggcgactcat tatgttctat tctgccgcca aattcacaaa gataatcagg
    74221 gtatgttggc cagtcatgag ggcgttgttg taatagaatt ttattatctt tggttaaaac
    74281 aaggcagcct acatattgat ttgggtagcg gtctatattg atatcgaaat tttttattaa
    74341 ttgaacagtc atagtataaa ggttatttgg ccaactcact attacctatt gttatttcac
    74401 cattaatatt ataagaataa caacccaaga attcgtctag ataatcgatg tttagaacca
    74461 gtatcgaaat cgtgtcatat tctatttcat atttcggaac aatgaataaa ttatttttta
    74521 tttttacttt atgtaaaacc catttatgtt ggctaactcc aggttctgaa gatttattgt
    74581 aatcaattac ccatattttt gcatttgtga caatcatagg aattataatc ggaaaatctt
    74641 ttgatttatc tttatctgta tccgttaatg atatggcttt tattgctgac aaaatttgat
    74701 attgggcttt gaaaaaatta ttttcagagt cgttcttaga tatttttttt aattgctggc
    74761 ctgttttgtt aaaaaaatca ccggtaaagg tgaagaaata ttgattttca tccggcttga
    74821 attgagcaat ccgataattg gaatcttgaa tagcgtgtcg ttttgtgttg tagctctttg
    74881 ggtcattgct actatcttct ttaatcaaaa ttagatgtga tgaatcagat gatcctttac
    74941 actcaattaa ccactcccta cctgtatatt gcgctgccct aatatcgatt tcaacaaact
    75001 catccccact tttcattaac tggttcatca aaacagaaaa atttttctct tctagtacct
    75061 tttttgttct gaattctaag ttaaaaccat atctgttagc tgcatccaaa attaattttt
    75121 tatcatccat aatttttcct aaattaccaa ttattaatcc aaaaatcagt gcacttccta
    75181 attattccct ttaattataa ttatagggtt acagggtgaa ttatcccaat tcatttatta
    75241 tttgtgctct acctttctgc ttaatccaga aactttccgt cgcacatagt tatcagattt
    75301 gagtctttgc cgctccagct gatcatgtgc attatccggc aataacactt tacttcttct
    75361 ggcgagctga accatatcga gttgggacaa gtctcgcaag ctgttttgac ttttggctgg
    75421 cgggccttgt ctaatgctgt cagcgttggg cttcgtactg gaaagtgttc ctctttctcc
    75481 aatggctgct ccagcagctt cattgcttct tggagttctt cctccatcat taaatctgta
    75541 tcgtcttgat tgttcatagc gtctctcctg ttctaagacc attttttggc gctgtgcttc
    75601 catttctgaa tcagcaaaag tgagcgggat attatgctgg atggttattt gaagaatgat
    75661 ctttttaaaa agtggcgagc catttacctg gatacagttg ccgtatctct gctgtgccat
    75721 ctcaatggct tttttcagtg tagcaataga gccgccctta gaaattttaa tttccttgcc
    75781 ggtgtttctg atgaccgcct tatccatttt atatatctct gttccctctt tagtaatgga
    75841 atcaacttgc ttaatatccg ctaaggaaaa attagatgac gcggctgata aggaataatt
    75901 actttgattt tttcgattgt gataacgcat agctgttaag gcttctttgt ctccttcccg
    75961 agctttttgc tgcaaccaat cagcccaggt tttgttttta tgtaattcaa gaagatgctt
    76021 tcgttctttg gcataatttt ggcgaacttt ttcaatttct ttcaaaaggg ttttgctgat
    76081 ttgctggtaa agatattttt tttgagtcct cggggctttc attaacttca aaactgctcg
    76141 tttggttcga ccacgtttct tggctttcgt aatcagtctg gctttagctt cacgcagatg
    76201 tttgagtttc tcggcaagca gcgttttgcc atgttctttt tcatataggt aacgcgcata
    76261 aatttctgag ccgatcactt ttctatttag tggttcataa cgataaatat tttgcctgga
    76321 tctttcagta ctaaatggtg atggcttaaa ttcacccaac ctggattcca ggttcttttt
    76381 ggaaaaacat cgtgaaacgg agcttgcttt aaccataagt cctgcttcat tataaaagac
    76441 cagaccatta gctttaatat gaatttccaa gccatgaagg gataggattt tatgaacctc
    76501 attccaatgg ttggcctggt taatctgttc cagacaattt ctctttatcc agttaatcaa
    76561 gctttcaatg ccagaatgtt gttccatatc atcggcaaga ttctctgaac gactttttcg
    76621 ggtttgatga ttggttattt caagaccata ttcaatttcc aatttcgatg ccacctctga
    76681 aaatgtccta taagctctat agggctcaat tatattaaag gcttttggat gaattttatt
    76741 gatggcaaca tgaatatgaa ggttgtctgt gtcgtgatga atggcgctaa tcctttgatg
    76801 atctttgaat ccaatagatg atatgatcct ttcctctata gctttcaata cctcgttggg
    76861 gggattttcc ccgggggcaa aagatatgag catgtggtag gttttatcgc ttttagctct
    76921 ttggtttctc gcctgagttg ccaatacttc atgaatagcc caggtaggat caacactgtt
    76981 acagtttgat atcctgacct tacccactcg ctcctgctta ttttgttcat cagtaatgta
    77041 ttttacaaga gctgaaaaac tgctcagtct tgctgacttc attggaatat ggcgaataat
    77101 cataactttt tcaccacttc aaacatggca gcctgcattg tctgaatgcg atccaataag
    77161 gcttttactt ttcggtgatc aaaatgtgcg acacgtctat cctgggtaag ccatagctta
    77221 aacagcccgc caagccttcc catgtctgca tttattttgg ataactggag aatgtaatct
    77281 ttgtctatgg cgctgtggat ttgatagccc aacccaatcc ggcgcaaata tcccgcaaca
    77341 gaaagtcccg cttgggcagc atgggattta atagaaattt cttcatcggg aaggacggga
    77401 acacgaagat ggcgaccgtg ttttctggaa ggtgatttat ttttatcgtc cataaggatg
    77461 ctgacctctt aacaagtggt ttgcaatcct ttaggtactg attaattgtt ttctttttga
    77521 tctcaaaaag caagataccc gttgagcacc gaaggtgcga ataaggtttg tgaagattga
    77581 tagccagctt gctggttagc taacttcacc tatcttgccc tgcttttcca gatcacttta
    77641 aagaatcata tcaggaactt tttggaactt gcaacgattt ttaaagaact tttttgccta
    77701 tagaactaaa ataatccaaa ttattcctaa aagttctttt ataatgctca aatactcatt
    77761 tttatattct agtaaattct taatagttct aaaatattcc ttttttcttg ttccaatcct
    77821 aattatgatt tagacttttt caataataaa attgacacca aagaagaatg aaaaagccac
    77881 tttccgaacg ggtaatccaa aatcagtcag aaaaaaaaaa cgggcgaacc aatgccaaga
    77941 ttcaatttat cgcactgaaa gaagacattc gagaagccct tgataagggc tgttcgatga
    78001 aagctgtttg ggaaacttta tctgatgaag gacaaatttc attcggctac aaggcattta
    78061 ggcattatgt acttaagtta attaagtcag agcaggaaaa catcagggat gataaacaaa
    78121 gcaaaagtaa aactaccaat gaaatcaaag gttttacttt taatccaata cccaacccgg
    78181 aggaactctt gtaatggcaa aaatacatat cacgatgcag ggcaaaggcg gtgtcggtaa
    78241 atcttttgtc tcggcaacta ccgctcagta caaacttaat aaagcacaat ccccactttg
    78301 tattgatacc gatcccatta acgccacctt tcatggcttt aagtcactta atgtcgaacg
    78361 cctgaatatt atggaaggcg atgaaatcaa tccgcgccat tttgacacct taattgaaaa
    78421 aattgcaaat acacaaaatg acgtgattat tgataatggc gccagttctt ttgtgccttt
    78481 atcgcattat ctcatcgcca accaagtacc caccttgtta aaagacatgg ggcatgaact
    78541 tatcatccat accgttatca ctggtggtca agcgttactg gacacaataa atggctttgc
    78601 tcagctggta aatcaatttc cagaagatat tcgctatgtt gtgtggctaa atccctattg
    78661 gggaaagatt gagagtgaag gaaagccttt tgagcaactg agggtataca aagaaaccaa
    78721 agatcgaatc gcagcaataa tcaatattcc tgatttaaag gaagaaactt tcggccacga
    78781 tctctcaaat atgcttcaac aaaaaattac atttaatgaa gcaattgatt ctcctgatcg
    78841 aaatatcatg actaggcagc gattaaagct gatacgggat caactgttta gccagattga
    78901 taatgctacg gtgatctaat gtcagataaa attaatgaag ctattcaata cattgcaggc
    78961 aagcatggtg ttgttctggg tagagatgat ccagttctta tcctccaaac catgaatgac
    79021 aggctgcttg aggaaaaccg aaaggctcag caggagatgc tagctcagtt aaaagaggaa
    79081 atggagaaca tttcctctca atggaaggtt gatgctaaag aaaaagctga gaaagtgctt
    79141 aatgctgcat tggctagcag taaagaagct atgaacaaaa tactgaggga ggccaccaat
    79201 gaatctgttc atgtcatgaa aaagctgata tcagattcat tgacagaagc aaatgatttg
    79261 actcaacaga cgcgtaaagc aaatcgattt acattgttag catcagcttc aatgatgact
    79321 gtttcttgcg catttatgtt gttcttactt attaattttg cgagataact catgcatcca
    79381 attggaacca aagatgaaga atgaaaataa taaaaagata caaaatctga gtacattttc
    79441 atggtctata gctgaaatac tgcgaggcga ttttaagcag tcagaatatg gtaaggttat
    79501 cttgccattt gttgttttgc gtcgattgga ttgtatttta gaaccttcaa aagatgctgt
    79561 aattagtgcg tatgaaaatt tacctgaagg gattgatgat catactaaag acatgatgct
    79621 atttagtgct gttggtggtg gcctgaaagt ttataactac aatacgctca cctttagtaa
    79681 aattcgaaat caagatccag gtgatgtaca taagaattta ttagactata tcaccagttt
    79741 t
//
LOCUS       DATNSN010000002        54740 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.002, whole genome shotgun sequence.
ACCESSION   DATNSN010000002 DATNSN010000000
VERSION     DATNSN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 54740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54740
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.002"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(195..515)
                     /locus_tag="VAH76_000086"
     CDS             complement(195..515)
                     /locus_tag="VAH76_000086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384750.1"
                     /translation="MMNDLAKILSLNNLLIKNNINPSENFETIYSQITDDGLKKEIES
                     RIIDYFSKLSLPEHVTIYDQLLLSLREKDAIFTFNWDPFLFDAYKRNSDIVSLPQISR
                     PLVF"
     gene            complement(765..1170)
                     /gene="ssrA"
                     /locus_tag="VAH76_000087"
     tmRNA           complement(765..1170)
                     /gene="ssrA"
                     /locus_tag="VAH76_000087"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1321..3336)
                     /locus_tag="VAH76_000088"
     CDS             complement(1321..3336)
                     /locus_tag="VAH76_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEO1384751.1"
                     /translation="MSTTVGLPQFSHIKIEHFKSHLDALLKNHLEEIDRLLKENHHYT
                     WDNLIYPLDSLADELERFWSPFAHMHAVMDSEAIRESYEACLPLLSAYDAAIGHNQDL
                     YEAIKSIDQHSLNPAQKKIIADSIQDFELSGVALSKANKKRFEAIQSRLAELSSKFEN
                     NVLDATHAYTIHITEAERVAGLPEHALNTAKELAHEKGLDGFVLTLEYPCFQAVITHA
                     EDRALREEMYRAYITRASDQGPNAGTFDNTPLIDEILSLRHEKAELLGFNNFAELSLA
                     TKMAASTNQVTEFIYDLISRTRDKGKTEFRQLEVFAQDKWNLSPVNPWDVAYLSEKRR
                     QDLYSLSQEELRPYFPQPKVMQGLFAIVKKLFGMSIEEIEGVDVWHKDVQCYCIVDES
                     NQTRGYIYTDLFARPHKRNGAWMDSMQSRRKLEDGTVQLPIATLTCNFAKPSANRPAM
                     LSHDEVVTLFHEFGHCLHHVLTQVDYLGGSGINGVEWDAVELPSQFFENWCWDEHALS
                     LLTSHVDTGETLPSALYERLIAAKNFQSAMAMLRQMEFALFDFRIHQEYQTGKASYVA
                     NILADVRSKTSVVPIVPYNRFQHSFSHIFGGGYAAGYYSYMWAEVLSSDAFARFEEEG
                     IFNPKTGHDFLKSILEAGGSRKAADAFIEFRGRPATIDALLRHNGIL"
     gene            complement(3436..4770)
                     /locus_tag="VAH76_000089"
     CDS             complement(3436..4770)
                     /locus_tag="VAH76_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384752.1"
                     /translation="MTPIISHLLKIFPELNTQVKTDSLNWTFNTHLKQLGPDFYSKVA
                     RLHPILNVEYSVLCQRLRHNLSSPDYSSLEQIKEQLIDALKLAELLEYTYQHYLVVPR
                     EVVRLRRHKAIYRELLTELSGYSFALDNPEPESLKTSLSLTQAIREKTAQSNWYRIFI
                     SRSKRVINLLDNLDTGSKAFRDFVVLLDKYTNPFLAYLGWCFFAPRLFTNLFLILKHT
                     IPGEWMGEKEKSLDWSDRFYAHLQRRWFELANDSVWFTVGILNCFVLVGALAPLSVYL
                     SLFAFVFDVANTSLRAYIEIGRLQQLQKEYGELFDQEENEDQKKIIKEYQDSISYRIK
                     FEILRSFLSVGGAVAVVIAMALSVPALGIINPVIPLIGAVLLLALWGVSLYLNNKLDE
                     YRPVDNIEKPAPSVINKLSFFAPKNEKKESLHLSCKPEKVLDAEELIPTFTF"
     gene            5028..7034
                     /gene="tkt"
                     /locus_tag="VAH76_000090"
     CDS             5028..7034
                     /gene="tkt"
                     /locus_tag="VAH76_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO1384753.1"
                     /translation="MNSFTELANAVRMLSIDAVNQAQSGHPGMPLGMADIATVLWKKF
                     LKFNPKNPHWFNRDRFVLSNGHGSMLLYSLLHLTGYNLDINELKNFRQLHSKTPGHPE
                     HGHTPGVETTTGPLGQGLANSVGMALAERVLASHFNHDHYNLVDHYTYTFAGDGCLME
                     GISHEACSLAGTLGLGKLIVFYDDNGISIDGKVESWFTDDTTSRFKAYNWQVIGPIDG
                     HDAAQIEQAISAARENTTQPSLIICKTVIGLGSSVAGSEKAHGSPLSAQDINNVREFF
                     NWKHAPFEIPDSIYKQWDHREQGEKEEQQWLQLLHEYRQQHPSEHDEFLRRANGDLPD
                     DWQEISAGFLKQCLNNEKAIATRKASQQCIEFFAPILPEMFGGSADLTGSNNTDWSGS
                     KAITAHNFSGNYLYYGVREFAMAAIMNGLAVHGGFIPYGGTFLVFADYARNAIRLSAL
                     MKQRVIYVLTHDSIGLGEDGPTHQPIEHIAMLRMTPGMTAWRPADLMETAVAWQQSLE
                     HHNGPSALLLSRQNLPALAHSADAADLIKKGGYIIADCEGKPDAILIATGSEVQLAIT
                     AAEKAKARGLQVRVVSMPCAERFLAQSDAYKNQVLPGQVRTRIAIEAASSAYWHQFVG
                     FDGAVIGLDCFGVSAPATDAFNYLGITVEKIINTLDTLTKQTMV"
     gene            7116..8108
                     /gene="gap"
                     /locus_tag="VAH76_000091"
     CDS             7116..8108
                     /gene="gap"
                     /locus_tag="VAH76_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO1384754.1"
                     /translation="MTIRVAINGYGRIGRCILRSIFEYNRQNEFEVVAINDLSGIDVT
                     AHLTRYDSTHGPFRSDVSIEGNMLIVDGHGIQVIAERDPAKLPWKQLDIDIVFECTGF
                     FTSRDAAMGHINAGAKKVLISAPAKGVDTTVVYGVNHSVIKASDVIVSNASCTTNCLA
                     PVVKVLNDAIGIESGLVNTVHAYTKDQMLLDGSHKDLRRARSATQSIIPTKTGAAAAV
                     GLVLPELAGKLDGFAMRVPVLNVSVVDLTFQAKRATSVEEVNDIMRKAKSRILQINED
                     PLVSCDFNHNPASAIFDTTETKVIGNLVKVVAWYDNEWGFSNRMIDTAHQMMNC"
     gene            8119..9309
                     /locus_tag="VAH76_000092"
     CDS             8119..9309
                     /locus_tag="VAH76_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO1384755.1"
                     /translation="MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTI
                     KSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLTGVDVN
                     PGELVVCENVRFNPGEKGNDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
                     AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIA
                     NTFLKAQGFEIGISLYEPDLLDEARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFN
                     KSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAI
                     AESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKH
                     VKTN"
     gene            9293..10717
                     /gene="pyk"
                     /locus_tag="VAH76_000093"
     CDS             9293..10717
                     /gene="pyk"
                     /locus_tag="VAH76_000093"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO1384756.1"
                     /translation="MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSAL
                     QLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGD
                     INGVSVAYPNLANELSISDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
                     KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPII
                     AKIERMEALDHLTDIIREADAIMVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVIT
                     ATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLFPVKVITMVNKICLS
                     AEKHASFFYHSDLETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHH
                     STVPIFAISANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGY
                     VLLTRGTQIGMPGGTNCMEIIPVV"
     gene            10809..16436
                     /locus_tag="VAH76_000094"
     CDS             10809..16436
                     /locus_tag="VAH76_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdhB"
                     /protein_id="HEO1384757.1"
                     /translation="MLLLSIEVIKMPDKKSSETLLSQQINEVRGLLLQALKTQLDDLV
                     AEKLIPQPSGVPFVDFEKDPPQIATLKKVINCLYHAEEAFKSWESIDTSTLLGKAKAA
                     PKLIHALTQIYKSLGLLDEASPEIRSVIADNYHLLEPLFTQAYAIVQESGWLSEFMEM
                     DVTDKASKVIFQGMDLLGPDVDKWHDTHPLVDAFSKISKLVETISTLQEKDMPGKEKE
                     QAVELIRALLDDLDNNPFISKLSISDFEDSKAIKDLLEWFKNIQDDGFDFSKKSIKQY
                     VSWANHYLPTLIAFADQLERQNYLKPGSLSANLCSQIEHLGKQVNDILSEPGFGVTER
                     VVTIDSLQGQREQKIHNAQVKSVQVIMATEKQLAATADFYQILEAYKGMNLSQITEPD
                     RRVLRQLYPQIQTALAHASLDLENQLTSVLNTTGPEKTPAKPKSWWELAKDGLGYVAS
                     YVVNYNVDKILGTRESVDRFLSSQIASEQFKILIAEKAREKLRTGSDKIKETALQYQA
                     EARLNAIKSTLRKQPSQMEAPPSEFVAVKPSKLVNLRGTLTAIQEMQLSTTVNGTRTS
                     VDTLIHRHLPEKYQACFSTLPYQINENDPELVKKIKTVENNLFQLEKALKDFEKIDLS
                     YGIMIRLHYFIAIASAASKLKSSVKELPPEGQKALAPLLQQIMTLGLSFSDTNYTAND
                     LSALQQLKMEGEVRPKSPSVASPDTIGTLPEETGKRTVKKPKEKTTELLDYADKISNA
                     RASLLNKFKNTLSPALADSLNPQKNGVPFVHLDQDPPQIAAIKKIINSMYHAECALKT
                     WHGIDTSTTLGKVAAAHQGVAALSQVYKSFTLFTEDFSDIHNLIRENHDLIAPVIIEA
                     NKLVNQYGWARQFSELDIAQQLGSILGQGINTVQPKTDEITQTASLVKLFSEIPRLLN
                     NISSSLDQKSEKSIDELKISNKKIEAIGAVFELFFEENAHLMSMFKGFSAISGLIELN
                     KKIQLEGTNLQEVTIRQYQSWLKEGYPNLMMILDEIETRHYLTPGFLSAPIALELDQI
                     NDKLNEIIETKPDFKLQKIPLSFYMGDKRIERLMTKKTEHCLAVLQIEEQKKAVNTFF
                     GILKHYSDQPLSNIKPNELAQLRLAFVEIQSAMANSNLELSNDFVAALNQLETSPPEK
                     TKKIQVTVSQLLKVQPAVETYLNEHKKSSELKIAVIDKAIEHVKDKSFELKSGASKEI
                     NIPQLRKTFLETESKKPVVGPGELKPTSVSSLNTVRGNLAYIQELKISSTVGMMREQF
                     SALTRDNFSNRVQKYLIKPQDKPLHMINDNDPLMVRQIKEVENGLYHLETALKQFEQM
                     KKSDSLVSQTKALLEIANHALQLKMAVERFSPELKEHYGPLVRTVLSFSNKIQSINYN
                     KEDWADLQFILNRAHKELLKRKTPRDHRIEKAFFEAGVREVQIDRDESVGKKAAKLGV
                     KYAHLASPQLEKARAYLSSRYKNAFGQQPKVIRSFTREQLANEEFMDAEISRLKETLE
                     EKYGFNIATIKVLLDLLEQIQRAGAQAAEVAGMVNTLVTYDFPKIKENAYRDLLTKLS
                     QEEDYLNLKPGTLINPAMAAVNQLFLSAALELDMPFSKKLSILDDTTYVNILIRQTES
                     DLQTLKEKLSEDPSNHEIAFEIEIKNDKLAFLRQQMVLLEDKNPNQTRNVLMDMQFEV
                     SLRNHLVYTTLKAPIAQEYEQIAIEYYKKNKNRFLYADDCSKELLQGLKEFEKKHLPD
                     HLIVFEAYDRLRLFSAKLPPKNQDVKEYIENINRELQNRDIPIRQRALKVKLLPNDTV
                     FIEKLISADKGTHFLRKFMQFITIITASITEAIKTGGSLVYIYRQKQMEQSIKNIEKS
                     ISFKEELHTIKDPDGPSPRKEEGQEGEIEFTKL"
     gene            complement(16536..17606)
                     /locus_tag="VAH76_000095"
     CDS             complement(16536..17606)
                     /locus_tag="VAH76_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384758.1"
                     /translation="MRIATLFSAVLFISPLWAAPKFFPSQPALNGVYDIKGTELCSTA
                     KHTLAYLNKGKNYDPQVIHDGKVIKISLDRVKKTLEFICQNQKKLNNPDFVKQHFDFI
                     RWYPDLRQAKQLSANKPLLQNIPKDKILMTKYFVHLAGASPKPSSAKPYPLYALPEDE
                     QNLTLDEANAKPGLIRFKYGKQAILSGALKNQPVSELAYLSRDDLEAALLQGTVVADF
                     GPTAGKKIFNVHRSNNITYDRTKTPYQQERFWYFKEVDGIKGYGKDAEYKITVNPKVT
                     FAADLAEFGLGKLLMIQYQDKSGRPMTKMGILADTGGAFNNNLYQIDYLAGSFAGKDA
                     LYRATRDLPDYVDAYFMILKQP"
     gene            complement(17607..18299)
                     /locus_tag="VAH76_000096"
     CDS             complement(17607..18299)
                     /locus_tag="VAH76_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="activator of prop osmoprotectant transporter"
                     /protein_id="HEO1384759.1"
                     /translation="MRKQALHPRTAVINKAQKNQSKRARSDALLWLAANFPEAFDNSL
                     RIRPLKIGIMSDILQHAEKAEQVGISKSKLREAVVLFTRRLDYLACLKAREVRIDLHG
                     NPVAEVTEEEAENASMKIKKRVEKSVKNARKQVNAKAANHSHVNNQPSTVSSVKPMNS
                     FDSHPEPLLPIYPLRSSTYASQNVAMQSAKSPSVVVKHKAPKQYDPDAVARLKEKLGL
                     SRKAEDKKETTE"
     gene            18656..18841
                     /locus_tag="VAH76_000097"
     CDS             18656..18841
                     /locus_tag="VAH76_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384760.1"
                     /translation="MRIILSILAIFMPWLVLLIKDNPGGAVVALIMQATLIGWPFASI
                     WAWRAVNPLTPASQSKQ"
     gene            19021..19752
                     /locus_tag="VAH76_000098"
     CDS             19021..19752
                     /locus_tag="VAH76_000098"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEO1384761.1"
                     /translation="MRTRVIVNGANGKMGILACETLENHEQFEVVAKLSRQDNLGQSI
                     LDTKAQIVVDLTRADCVYENSLTIINHGARPVIGTSGLVETQIDELTKLCEIKQIGGI
                     IAPNFSLGAILMMMLATKASEYFSEVEIIEGHHQQKLDAPSGTALKTAEMIAAARKKP
                     KNKLPLKELTPGARGGSHHDINIHSLRLPGLLARQEVLFGNIGETLSITHNSIDRHCF
                     MPGIVLACQKVLNLTNLVYGLEHLL"
     gene            complement(19856..22324)
                     /locus_tag="VAH76_000099"
     CDS             complement(19856..22324)
                     /locus_tag="VAH76_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEO1384762.1"
                     /translation="MGKGIILRVLENTILSPQVFDTLERLLPGYKVEYFKEQPDYRKS
                     IARRIDSLHDAFSFILKAYPLDPKHTSLTVATLSTYAAECKASCDLEKLTLEELHLEL
                     ERFTAKLVEAIAIAWKWPKGKAVKEAIASLNEAEQYVLMSRGRSDIATIMPIEMGSET
                     KYVLQYDESLSPVYEQWLTELKQLKEYNFPKTPAWFKNLPPYQQAYYCNLNLSSVDPK
                     KALQHFNTFFGNWGDIAKRSLNLTTELNQIHTNSPPYPSWFNELSPAQQAMIRVLSAT
                     PHEIKSSLKEFKKFMVEQARNDQYASTLSLVPKLPQWYWVLSEQQQYFLEYALKNAEK
                     VEDVVSYLSSRHRTLPAPANYGAHSLYLIDGEGKETLFYDKRYRSSHVASRDSLKFPE
                     DVQQRHVDSNLVKVMEFAKPQQPLLLQTLISPIHAVDYIPTVVTDFLPELPPDLELYK
                     IAREAVTRSKRRHEIFQHNHPFNIAKRYYYTQATDTDSEFLLKAAQKYVSSKLGLQAL
                     IDDYKAVLESPLGSATFWDYDGRELFLSSLEELIILNMGGYSYGSCVSGKDRKAVELL
                     HTDAMILYKAKYGNWPKFGIPKEKQERVNFINIVVDLYISRHQHELAGQNAPGSEGIK
                     TPDWYWPNDIAEAINERLGTEKALAYDDRLATDNEVKNISKDLRSFFLPENELHCLLI
                     AKQLGEKMCTMLYDVLSALINEERRFQKSSKDSWKLRWFSDKDVSSTPTGILNIREVM
                     HDENSGNDNVLRIGKIFAAVLNRPESDSSRTTATNSVYDRIRKLLQPLSSESTLQTLA
                     EEAILEWSSLFESSKRENSGLVYM"
     gene            22502..22678
                     /locus_tag="VAH76_000100"
     CDS             22502..22678
                     /locus_tag="VAH76_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEO1384763.1"
                     /translation="MKSIEDFTGFSESGIDDAIQNALKKAGNPTHFEVVETLGSQDSQ
                     SDRRYRVTLKTLSD"
     gene            22789..24288
                     /locus_tag="VAH76_000101"
     CDS             22789..24288
                     /locus_tag="VAH76_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO1384764.1"
                     /translation="MGYNVPHYIGGQLINETDTEAQAIYNPALGEIIGQVHFASKATC
                     DRAVAIAKAAGNEWSQTPAIKRARVLFRFRELLEKYQMDLARIVTREHGKTLDDAKGS
                     VARGIEVVELHCGLVNELKGDFSAEVASHIDCHTFRQPLGVCAGVSPFNFPVMVPIWM
                     MIPAIACGNTFILKPSEQNPSAPVRLLELLSDAGLPPGVANCIQGNKTTVEHLLTHPD
                     IAAFTAVASTPVAEYIYTTAAAYGKRAHTFGGAKNHCVVMPDADLDQAANAIVGAAYG
                     SAGERCMALSVVVTVGQHTADALINKISPLIRAIRVDAGDVEGTDMGPLVSQAHRQRV
                     LAAIEKGVNEGARLIIDGRTFQHPQYPQGFYLGPCLFDEVTEEMSVYQNEIFGPVLVI
                     VRVNHFEEALALVNKHQYGNGTAIFTRDGFTAREYSQRVQAGLVGINIPIPVPIANHP
                     FGGWKRSVFGDTNMHGEESIHFYTRRKTVTSKWPITELKDSSFAMPTHD"
     gene            24312..25208
                     /gene="mmsB"
                     /locus_tag="VAH76_000102"
     CDS             24312..25208
                     /gene="mmsB"
                     /locus_tag="VAH76_000102"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEO1384765.1"
                     /translation="MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQG
                     GAIAHNLQELAKDKDVIVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSS
                     RETHQIAKENHLLVVDAPVSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
                     TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSK
                     YVPVPGILENVPANNDYKPGFAANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFV
                     DQGLGDIDFSAIIKHIGKTQEV"
     gene            25210..27093
                     /gene="acs"
                     /locus_tag="VAH76_000103"
     CDS             25210..27093
                     /gene="acs"
                     /locus_tag="VAH76_000103"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEO1384766.1"
                     /translation="MLPVHLKHPHLTPTEESLSEFWSEIAAQTIDWIQPWSITLQGGL
                     HKGDVKWFQGGLLNVSANCLDRHLPHKANQTAIIWEGDDENQNKTLTFAQLYSEVCKM
                     SNVLKSLNVRKGDTVGIYLPMIPEAAIAMLACARIGAIHTVVFAGFSAHALQQRLTAS
                     SCKCLITVDAFQRGGKTIPLKKQADEASVDLNITKLVVKNSNVPMALNKNKEHWWHEL
                     KQTVSDQCTPEPMNAEDPLFILYTSGSTGQPKGVVHTTGGYLVQAAYTHQLIFACQDN
                     EVFWCTADVGWITGHSYVVYGPLCNGITTLMFEGIPTWPDAARNWRIIDKHQVNVFYT
                     APTAIRSLMRAGDQWLNSSSRNSLRLLGSVGEPINPEAWNWYHQKVGQGKCPIVDTWW
                     QTETGAIMISPRASDEVIKPGSARKPIPGIIPLLLNEQGHEINGSGEGFLAIKYPWPS
                     MARTIAGDHQRYCNTYLSNGYYITGDGAKRDEDGDYWITGRIDDVLNVSGHRLGTAEI
                     ESALVSHPKVAEAGVVGIPHDLKGQGIFAYVILKQGNKPDAKLQTELMERVKDEISAI
                     AKPDVIQFVNDLPKTRSGKIMRRILRKIACKEVSHIDELGDLTTLANPQVVEELMKNV
                     LRQ"
     gene            complement(27190..30501)
                     /locus_tag="VAH76_000104"
     CDS             complement(27190..30501)
                     /locus_tag="VAH76_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system substrate"
                     /protein_id="HEO1384767.1"
                     /translation="MTPLFPTQGPITIRQGIGGSCYLLSSLDCILNLGKDGEQLIKSL
                     FTQTEDGKVIVRIKRHEALKNNLQKNKMTGKYTHYVDELNNEDVFEISPERLKEIDNQ
                     YGGVKSNSLAIKILERLVSYYYAGDWSNTNPLASVIAHDIPDRIAGFTSTAFLGKFFG
                     IEAEDIPYSKLDDIIKLKLMNPDEPVYISMSYGKVDGFGKFHGRHALRIDKIIPKGHG
                     DYDFVLINPHDNSKTETYKLDDLNKRNCRFCLFNTSIHRASLTKKLLTLSNDEGRYVF
                     SNSGLQKRLISLEEMNLLTDNKIISSCISLHKQIPYLEKLFLKLSVEEKKTLIACIAN
                     ADGSKKEFLKLFLTHIPAMDLLELVLREETSQELLGEVLAELALSSRVEENKLSPQAG
                     INFNSEAFLHLIVKSAIQQKINQFAYTPEKAKQEIESGVINFYFGGASSNLTRASGLR
                     ALFIANVFSKKSIETLFPPKALFAKAIANYLTLKTLPDLLIEYLKSQDTSPIDEEFFD
                     IVLTSATFKDPDELFENLFRLSQISPEVAKALLVFASQKINVLFGISLEEYAKKIALK
                     DSGEFKSWFESLSNPQPAIQIPEIDKVLRQQRVEDAKRVISDIVQRINSFSFSFEGFK
                     TVAHLNLNAEELRSQLKKIVHSGELQNALQILDLPDGHPEVQKALERKLRMIDVAANR
                     RLDFLKKYEADIDEHVRRIKDFPIDFNGAGTIVAIESQRILLNKRLHTLVKAEDLLGE
                     RLIANPKIKMVYFAQVEKINLRAELLQKQLLDEAQKVIDSVEKRIDNFVIRFNDISTS
                     SAVEWQRNNLLQQLDNLVKPNQALLGAEKVLDCNDLQPSIVRALQAKKQEINETADQL
                     IIKINAEEVVKSYEKQIREFPISFNRCQTVEEVITRKQDLIQSVRNLVGNKPDLLKAQ
                     EQLQLLSGEYHSDIKMALTDKVREINRQADAVSKRITDQIAATKETLNILAEIKFSDH
                     LKIIESMVKTLEAKAVGDKNYKRAAPIARAFYNNLLMAEERFKNSQLPKNVKCKDFHQ
                     ACARAINAVIPVLEIHRGWKQVFADLASALVTLCTLGGANLYAGRWRLFPVPTESEKI
                     VKDFSVSMQPLAVRA"
     gene            complement(30615..31217)
                     /locus_tag="VAH76_000105"
     CDS             complement(30615..31217)
                     /locus_tag="VAH76_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO1384768.1"
                     /translation="MPINLYSKAVFLKSAARVNQLPEDSGYEVAFAGRSNAGKSSALN
                     CLTNNKNLARTSKTPGRTQLINLFSLDEQRRLVDLPGYGYAKVAMEVKLEWQKNLAHY
                     LEARQCLRGLILLMDVRHPLKDLDQILVNWALHRELPVHILLTKADKLSRSEVKNAVL
                     KVRQYYELAEHLVSVQAFSSVKKDGVEELISLLDRWYEWN"
     gene            31292..31894
                     /locus_tag="VAH76_000106"
     CDS             31292..31894
                     /locus_tag="VAH76_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEO1384769.1"
                     /translation="MKKLALVLILCSSSMIFAQENPQEAGQSKSTVCTACHGPQGIST
                     NPEWPNLAGQHEKYFVKQLKDIKEGKSRSAPTMTAIVANLNEQDMDDLAAYYAKMPVA
                     EGSTPKKYLKRGEQLYRGGDLNKHIAACIACHGPRGSGNAQAGFPLLSGQHAAYTVMQ
                     LQAFKDGKRTNDLNQIMQDISSKMSPEDMEAVAYYIQGLY"
     gene            31905..32519
                     /locus_tag="VAH76_000107"
     CDS             31905..32519
                     /locus_tag="VAH76_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEO1384770.1"
                     /translation="MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPL
                     ITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERIPVVFKPNWDLYAKAYYTAKTLAM
                     SDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
                     LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA"
     gene            complement(32679..33977)
                     /locus_tag="VAH76_000108"
     CDS             complement(32679..33977)
                     /locus_tag="VAH76_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO1384771.1"
                     /translation="MPETIINIENLSKSFKKAPSQHLLVLEDVNFKLQEGEIVALLGK
                     SGSGKSTLLRIIAGLIAPSSGTVTYRGKPVTRPVEGIAMVFQSFALMPWLTVLENVEL
                     GLEAQGISREERRHRAIEAIDIIGLDGFESAFPKELSGGMRQRVGFARALVINPDVLL
                     MDEPFSALDVLTAENLKSDLLELWKEKKTNTNGILLVTHNIEEAATLADRIVIFGNDP
                     GYIRAELPVTLPQPRDPESPEYLALVDKIYTLMTTGPKEKAKRAQRERQIGLGYRLPD
                     VEPSELSGLIETMKSFEERIDLPELADELMMNIDDLFPILETLEILGFAKVSAGDIQL
                     SELGKQFSEADLQERKQLFAQRLLEKVPLARYIRRVLDEKAGHRVSEERFLSKLEDYL
                     SEKEADRVLKTMIDWGRYAEIFAYDFNTGILSLENPGKGA"
     gene            complement(33985..35724)
                     /locus_tag="VAH76_000109"
     CDS             complement(33985..35724)
                     /locus_tag="VAH76_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO1384772.1"
                     /translation="MGNSRFYFANPDKFSRFINRWDLLLLILTFSVLFFLGWAGSQMA
                     TPYQLGDQIPISLEPSNLPFYALRTVLRMFIALIFSILFTFIVGALAAKNRRAEQIVI
                     PAIDILQSIPVLSFLSITVTGFIHLFPNSLLGPECASIFAIFSAQVWNMTFGFYQSLK
                     TVPHDLIEVSAMFRLSAWQRFWKVEVPFSMSGLLWNMMVSMSASWFFVVLSEAISVAH
                     QNIRLPGVGSYIALAIAQRDLHAVGYAILTMVIVIFLYDQILFRPLIAWSEKFKMEQS
                     PDESEYQSWLIDLIRSSRLMKRVTEWLAVFTNSFVNARWLRIGEVKAVKEIDFKRQKR
                     LDRLWTALVLIAVITGGWFLLKFILAELKVSDIFHVFLLGAATGARVIILILLSSMLW
                     IPVGVWIGLRPRLAQKIQPIIQFVAAFPANLFYPLFVIAIVKFNLSVEIWVTPLMILG
                     TQWYILFNVIAGASSIPRDLYLAADNFGLKGWIWWKRLALPGIFPFYITGAITAAGGA
                     WNASIVAEYVSWGNITLKATGLGEYIQASTTAGDFPQIALGTAMMCVYVLAFNHLIWR
                     PLYRLAEERFNFN"
     gene            36221..36751
                     /locus_tag="VAH76_000110"
     CDS             36221..36751
                     /locus_tag="VAH76_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEO1384773.1"
                     /translation="MNKTITIALSLLLSIASKLTYAGPDRAQLAVWANEAIIATYTFD
                     YKNYMQQQKEIAKYFSADGWIAYSKALNQSKLPEVVQKNAYFVNAVATEPPKLITLDP
                     THWQAIMPILVVYKNPQYEQKQNLKVILGFTVASPGQGVRGFSITSLQSTPTSPPCQC
                     KFEETPENTKQGDAKQ"
     gene            complement(36726..37193)
                     /locus_tag="VAH76_000111"
     CDS             complement(36726..37193)
                     /locus_tag="VAH76_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEO1384774.1"
                     /translation="MVWPIIWRWILDLGWLCFLLILFWYFWKKRRDLVEARSWLKAKG
                     HVTRCEWTRVGHSVWPRIEYIYEVYEKDLTGEYLFLDTTFNTPNSKYSRSIAYKVAIA
                     YRDNSEIDVYYNPNRPEQSALDVTIPKKLTFILILISSLILLHIGIIVWRLLV"
     gene            37329..38411
                     /gene="gcvT"
                     /locus_tag="VAH76_000112"
     CDS             37329..38411
                     /gene="gcvT"
                     /locus_tag="VAH76_000112"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEO1384775.1"
                     /translation="MTAKTPLHTTHLACGAKMVDFHGWDMPLHYGSQLNEHHAVRNDA
                     GMFDVSHMTIVDILGAGGRQFLRKLLTNDVDQITHNGKALYSCMCNEHGGIIDDLIVY
                     QRASDNYRVVLNSATRQNDVAWIRAKSEGFAVGLQERRELSMLAVQGPNAIAKTLSIL
                     APAHVDAVSTLTPFECVDVDHWFFARTGYTGEDGLEIIVPNEFITQLWNDLLNAGVTP
                     CGLGARDTLRLEAGMLLYGQDMDETTTPLESGLAWTVKWEPEDRGFIGMGALVSQKQQ
                     GIKRKMVGLTLLDKGIMRHGQKVIIEGCPDGIITSGSYSPTLQQSIALARVPVETGEQ
                     VLVDIRGKLIPAKVGKPRFIKQGKPV"
     gene            38445..38822
                     /gene="gcvH"
                     /locus_tag="VAH76_000113"
     CDS             38445..38822
                     /gene="gcvH"
                     /locus_tag="VAH76_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEO1384776.1"
                     /translation="MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIG
                     DEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKP
                     SHPDEIKSLLSDEQYQNEIAEEN"
     gene            38825..40195
                     /gene="gcvPA"
                     /locus_tag="VAH76_000114"
     CDS             38825..40195
                     /gene="gcvPA"
                     /locus_tag="VAH76_000114"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEO1384777.1"
                     /translation="MPYIPHTPNDTKEMLEAIGAQDIQDLFDEIPASLQYAGFQNIPA
                     GINEMEMLKEAQNQAQKNRNGICFIGAGCYEHHIPAAVWDIASRGEFLTAYTPYQAEA
                     SQGTLQLLYEYQTMICELTGMEVSNASMYDGATALAEAVLMAVRLNKHSKTNRVLIAG
                     TVHPFYRETIETIVRNQHIEVITLPFDEQQGITDLGSLNQYTGEDITALVIAQPNFFG
                     CLEQVDKMTSWAHHNKTISVACVNPTSLALLKPPGSWGEHGVDIVCGEGQPLGSPMAS
                     GGPYFGFLSTRMAHVRQMPGRIIGRTVDKDGKTGFSLTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYMSLLGPEGLSQVATQCHQNTHELITALTQIEGVELAFKAPFFHEALI
                     KLNQPVQYVLQQLADAGIAGGYAPEQHYPQLANTLLVCATEVRTAEDIAKYAKTLKTI
                     MSKRGA"
     gene            40195..40503
                     /locus_tag="VAH76_000115"
     CDS             40195..40503
                     /locus_tag="VAH76_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HEO1384778.1"
                     /translation="MFIVQLTYLVPINEVNKYLQAHREFLDYHYKQGLLVASGPMKPP
                     TGGIIIAATNDRAHLESVFKQDPYYLAEIAEYQFIEFTPVKHRNELKELIQKTEGKLC
                     "
     gene            40497..41951
                     /gene="gcvPB"
                     /locus_tag="VAH76_000116"
     CDS             40497..41951
                     /gene="gcvPB"
                     /locus_tag="VAH76_000116"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEO1384779.1"
                     /translation="MLIFELSKTGRQAKAQIPRAVGKNYSIPEEFQRKSPPRLPACSE
                     LQVVRHFTCLSQKNFSIDTNFYPLGSCTMKYNPRGVHKAASLPGFINRHPLAMDNESQ
                     GFLETLYKLQNYISEITGMPGVSLTPMAGSQGEFAGVAMIKAYHQSRGDTARDEILIP
                     DAAHGTNPASAVMCGFKVVEIATAPDGDIDLDELKRKVGPRTAGIMLTNPSTLGLFMR
                     QIKEIASLVHQAGGLLYYDGANLNAILGKVRPGDMGFDVMHLNLHKTFATPHGGGGPG
                     AGPVAVGKRLIPYMPLPVVKKTDSGYHWATRQDYPQSIGRLSCFMGNAGILLRAYFYM
                     LVLGKEGLLRVSEFATLNANYLLKELTKVGYTAAYPDRRASHEFILTLNSEKKNYDVT
                     AMDFAKRLLDYGVHAPTTYFPLLVPECLLIEPPETESKEELDAFVAVMKTIREEASKQ
                     PDILKTAPHTLPVKRLDDVKAARELDLNYFATHE"
     gene            42156..43400
                     /locus_tag="VAH76_000117"
     CDS             42156..43400
                     /locus_tag="VAH76_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEO1384780.1"
                     /translation="MVVELDEPPVRTLLARQGIYDKNSAIFAYELLYRNSEAQNSQVD
                     NLNPLSGEAATSSVLLQLFANLDVNTIIGNKQAFINFTHNHLVQKIPMLLPKNRIVIE
                     VLETVAIDQALLLNLMELKKQGYQIALDDFVFRNELAPLVDMADIIKIDVLHLDQQQI
                     AEQLLPLKSFRGKLLAEKIEDKQQFNHCVNLGFDFFQGFFLNKPDSLTGQIITENKIQ
                     LLRLLTEINNEEVPIQRIEEIILQIPKLSYRILRLANSASLYMSKKIESLMDAISQLG
                     LMQIRNWLNLLLLASLDDVAPDLLERTLIRAKMCESLAKSMDYPNSHQAYTVGILSTL
                     DGILNEPMPSLLAKIRLSEALNEALLNYKGDLGKILKFAIDYEQANFNQLERIPVKSE
                     TLSQSYLKGIEYANHVIDIINK"
     gene            43637..46858
                     /locus_tag="VAH76_000118"
     CDS             43637..46858
                     /locus_tag="VAH76_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384781.1"
                     /translation="MIIRELFNWIHNDSATLHLSDQTIERDLIEQEESQAKQAHRVLL
                     GNARLLKYSGSSNTEAIKELEQHCLFMDYLQLYKQEFVKDRKNTVFLIALKNLLSHPH
                     EEQLRLMPKINELFQILLRDNKESALSFYDKNKELFRHCTEIQERVTFELLQQKMEAD
                     SKLVMTQLDYFNEHLAYQENPLGIIALCRNWIGETEKFTALILWLLERKVSVEKILLT
                     NLLQDFLKYHLFTLHSKDSEVSRLYSLLGHFPEAQELVMAAQKISCGELMFQQYSLDG
                     IFRGKNLPVVSPEIPPLQFSLRKDNFNALYRTFGQYFLTAGVATATNHSDVAWLDMLR
                     HTLNQPETLKLLPDIINIIAREYSPKVLKTLAELIAESTAHRLLTLNQSCVFHLLQHK
                     PRLLHDITEENVIEYINLLVRLDTHDQEIIYQLMALFRVLLKKNHPATKAVFEAILDN
                     LVNHPQLLEDEEFLTQLKKYPDCELLIEERCENILKQLNFCIAEQTSGLLFGKHNYYI
                     IEDVWLGALRKFAVLQQINPQMKSSFGHKYALQARVAEVVFIHQGDLFDLDTFIDALD
                     LPPVTSSGEISPYERALIEILATIDNDLIRKQIIQKLETTPFNRLDWYEREYGNQTVF
                     IKAAKKGNLGLINLLEDKMKPSVLNKALRAAAKNYQWETLDHLCSLPGVELRQDEMDD
                     LVVYLAEHGRIESVKKLLKLYDYKPSTELIGTILKKAIDNDNLQVVMYFCKLPVESPK
                     QAMLDRLFKLAIQLQHWGIVEYLANSKHYSPSQATLEKAFQQTALAMQHEAVEILGNV
                     EKTPIRAIVIERALLKASKLGHAKVVQSICALPSESLTKQAIEDALEQAAAEGHLDIV
                     SCLCEPGTTTLKQPGINSGMKIAVQAGKLSVVNYFCSMTGSNKPTPRLIDQTLVMAAK
                     KGQTAIFIAIHSNHQTPPGKHAIEQSFQLAITAGKLPILDYLCRHEGYGLNQSKVDQA
                     LISAVKSKQLEIVSYLCESLEITPSRKALRIAVSKAISSDQTGLAEYLRSRSTDKSKL
                     TDTLVDEIDSTSKVGKQLEANGLFKKKKRQTDREPQILFNPAI"
     gene            complement(47158..48195)
                     /locus_tag="VAH76_000119"
     CDS             complement(47158..48195)
                     /locus_tag="VAH76_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEO1384782.1"
                     /translation="MVMRSFSFYYQHLENVSRSFSFCISQLTSPAKEWVALSYLLLRV
                     ADSIEDAKWQDLQAQSDSFAAFKSFLVQTPANDQFTKWLSSFPSQLPLGEKQLLCAVP
                     LLLEEKDNLPESIKQSVIKTILQVVDGMHYFLNHYHMEGKIVFPSLVTTNQYCFFVAG
                     LVGKLLSHIFTDLLSNFKWTASLLNQAFHFGFFLQKINLLKDKVDDETSGRYFISSEN
                     SLRESLVIHAHHSLAYVKSIPIIEGRPYRLFCAWSLFIGLASLKWLDKYGHRQKIKPR
                     ETYYLVNQINLLIDDNRALEKLFNSYLPGQYEEGSRYASESTKKIPTWFKKIYDGEVD
                     SLTLFDLDIEV"
     gene            complement(48195..49997)
                     /locus_tag="VAH76_000120"
     CDS             complement(48195..49997)
                     /locus_tag="VAH76_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEO1384783.1"
                     /translation="MIQLGDLLKPTWAAEQSLNNAWSVLNDEEKETIKSRMDKIFYNE
                     IPFQLEHDKLIYIHLFSLFTQLETIGLRGLIKSLEKLRGTDLYQQMRQQITDEIFHAT
                     VFAKVAFQLSAPYALPLGHQKSIKHFISSLEGEEDLATSITLVNLVGEGWVEELCIAM
                     KEKNIAVTIFETVLEDESRHMDEYDLYRQIGLPNKDYLRKKLAIFEDELINTVFAHEQ
                     YLTTLGILLGKEGALKLLNNINNKHHWMLKKIGLTPSAHWQLFMDTMPLLMKNLSHDF
                     EKDKAIEPTNIRKLLSAIWNDPELPTESAIFNINVTPVCFFEKKFKPETITCLMLQAL
                     SKACFDNPQTRNYIFNHKLYHSHNSYVALAVKIPGSDQLGAIEFKNCHEMTMTELAQH
                     IQHDMRIMKYCYEKTQSLQKEHPYLIEVVNRLLTPRHEREYRDFLFARPAISLSNIGH
                     WGYQAAVSPLFPNETFKITLTEIERKQVWSKTNNTFEVQDVLPVGMSVDHRVFDGNIP
                     FPRYMQEAFDQMFQDMEQSRIKPLSKPFSNLDNFIKYSNTLLENDLEFGFLYLFSLMH
                     VWKNYISYDELSKTVEENYERIKQALSKSEHQLG"
     gene            50636..50905
                     /locus_tag="VAH76_000121"
     CDS             50636..50905
                     /locus_tag="VAH76_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384784.1"
                     /translation="MNSKVFSQRFNRELATLGFPEDLTDKTKAVSKVFGVTRHLANAM
                     IFGHVLPDKEQLDKIAEILEVCPQWLSGATDRKKAYSGRETADSV"
     gene            51004..51810
                     /locus_tag="VAH76_000122"
     CDS             51004..51810
                     /locus_tag="VAH76_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEO1384785.1"
                     /translation="MECLSFCVAKTIDLTRLDLHLKNVSKEFSAVKTRDVIRLNSHRN
                     KDHTLFIFKNGTVVSWGVKRYQIHEYLDIIKLLVDKPVALLVHDEFHYQIGDKTAIEP
                     HGFYDVDCLTIEEDSDELKLSLSYGFSQSVKLQYFETIIDALIEKYNPLIQALSHKGE
                     MPISRKQIQQVIGEILGAKSELNLISNFLYHPKYFWQHPTLEEHFSMLERYLHIQRRV
                     NAINHRLDTLNEIFDMFNGYLESRHGHHLEIIIIVLIAVEIIIAVMNFHF"
     gene            51828..53108
                     /locus_tag="VAH76_000123"
     CDS             51828..53108
                     /locus_tag="VAH76_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO1384786.1"
                     /translation="MPTLKSYFLKDTSLRTEILAGATTFLTMVYIVFVNPKVLHDAGM
                     DQGAVFTATCLITAFACALTGFIANTPIGVAPSVALNIYFSYSVVQGMGVDWQHALAM
                     VFISGLLFLFLTLTKLRRLLIEAIPHNLQIAIVIGISLLIALIALENNQIIIGDQHNL
                     MRLGPIIRPETGLFFLGFILILTLDYHKIPGAIIIGILSISILSLMLGLTSWQGLIAM
                     PPSLEPTFLQLDFSGLSSIVALKTIFTFFLITVFDATGSLIGLLSPTIFKHEKNYYKR
                     LTRSLTADAAASSLAGLMGSASTSPYIESATGIEAGGRTGLTALVIAAGFILMLFLFP
                     LAKMIPSFAVGPALLYVACCMIKHITNMKLQDITETAPCMVIIMMVPFTASIADGIGL
                     GIILYTLLKLFTRQKPNPLIIVLSLVFIVFFLIN"
     gene            53432..54028
                     /locus_tag="VAH76_000124"
     CDS             53432..54028
                     /locus_tag="VAH76_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEO1384787.1"
                     /translation="MLEQSLRHYYQLVLVDPILPALRDRITPLSITWLSGIFGLLFIP
                     FVLLDKPYIAISFLLFSGYLDTLDGSLARFQNRASDFGSAMDIVMDRIVEFSAVLALY
                     LFDPQHRATLAILMLGSILLCITSFLVVGIFSNNTTQKSFYYSPGIMERAEAFLFFIF
                     MTLFSNCFNFLAALFCFLVCLTALIRLFEFKKSVRAPG"
     gene            54135..54710
                     /gene="msrA"
                     /locus_tag="VAH76_000125"
     CDS             54135..54710
                     /gene="msrA"
                     /locus_tag="VAH76_000125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO1384788.1"
                     /translation="MLRIFLYFLLLINTTFCLAKTEKAIFAGGCFWCMEADFDHLPGV
                     LSTTSGFDGGTLKDPTYEQVSAGNTHYAESVMVEFDPNKLSYKKLVEYFFRHIDPTVK
                     NAQFCDHGSQYRSAIFYLNDQQKKIAMEYKKELEQKFPTVYTEIAPSTSFYPAEEYHQ
                     NYYKKNPIRYKYYRYRCGRDARIQEVWGNKK"
BASE COUNT        16979 a        11235 c        10670 g        15856 t
ORIGIN      
        1 catggcatat aataattact cgcatgaacg tctaggccaa cgccataatc ttgaattttc
       61 gaaagaattt tgcgatgaaa gatgatgagt tggagtaaat gacttccatt tttcactaag
      121 aatcttagta ctaaaaatat caatgacttc aacatgctca aattggcgtt ctgaaatgtg
      181 aagccacgct ttattcaaaa gactaacggc ctcgatatct gaggcaatga aacaatatca
      241 ctatttcttt tatatgcatc aaataaaaat ggatcccagt tgaatgtgaa aatggcgtct
      301 ttttctcgaa gtgataataa caattgatcg taaatagtta catgttctgg taatgaaagc
      361 tttgagaagt aatctattat cctactctct atttcttttt ttaaaccatc atcagtgatt
      421 tgactataaa tagtttcaaa attttctgat ggattgatat tgtttttaat taataagtta
      481 tttaacgata gaattttagc cagatcattc atcacaggta attcattacc tgaccataat
      541 tgtcattcat tttgtaatgt cctttagtgt tttttaaatt atcttgccct agttttttgg
      601 gcagatattg ggtacttttt caaaaatttt gggcatattt tgggtgattt ttgagccaac
      661 agaaaaccac aaaagacaac cccatcaacc agaaaagtgg atttaactcg ttgatctaca
      721 agggtttatg atggtctttg gtggtcagga atggttttga taattggtgg aggcggcggg
      781 aatcgaaccc gcgtccgcaa gtcctctgcc tttagctcta catgcatagc cttgtctttt
      841 gaatttaact gtggctcctc cgacgggcag gatgatacac agcgattccc tgaatttcgc
      901 gccataaccc gggatagatt aggacgctag cttatctctt atgaccgttg attcgatacc
      961 gataagcggg ctcggtcgaa cgggatactg ggtttattgg ccagtacaca gtgatggttt
     1021 aactaaagtg cttattaggc agcgatagct tccccaccag caaagttttc atcgtttgca
     1081 tttatattta attgagtctt atttacgaga gatctccttc tcggcatgca cttccggttt
     1141 tgcaacccac gtcgaagcca ggtcgccccc cattctgtac actttaatta taacataaaa
     1201 tgccggcatg taagcaaaat cccgccagca taccgaatat attttcaaga cttacggaaa
     1261 accccacggt caaggcaaaa aatgttttac cgagaggatt ggggggcgta agtcctgttt
     1321 ttataaaatc ccgttatggc gcagcaaagc atcaatcgtc gcgggtcttc ctcggaattc
     1381 aataaaagca tcggctgctt ttcttgagcc gcctgcctcc aaaatggatt tcaaaaagtc
     1441 atgtccggtt ttggggttga aaataccttc ttcctcaaag cgcgcaaagg catcgctgga
     1501 tagtacttct gcccacatat aactgtaata accagcagcg taaccacccc caaaaatatg
     1561 cgagaaacta tgttgaaagc ggttataagg aacaatgggt acaacactgg ttttagaacg
     1621 tacatccgcc agaatatttg cgacatagga cgctttcccg gtttgatatt cctggtgaat
     1681 gcgaaaatca aacaaggcga attccatttg tcttaacatg gccatggcgg attgaaaatt
     1741 tttagcggca atcaagcgct cgtacaatgc ggaaggcaag gtttcccctg tatccacgtg
     1801 cgaagtcagt aaagacaatg cgtgctcatc ccagcaccag ttttcgaaaa attggctggg
     1861 taattccact gcatcccatt ccacgccatt aatccctgag ccgccaaggt aatcgacttg
     1921 agtcaaaacg tgatgcaggc aatgaccaaa ctcgtgaaac aaagtcacca cttcatcgtg
     1981 cgataacatg gccggtctgt ttgcagaagg cttggcaaaa ttgcaagtca aggtcgcaat
     2041 gggtaattga actgtgccat cctcgagttt tctgcggctt tgcatggagt ccatccaggc
     2101 accattccgc ttgtgcggtc tggcaaataa atccgtgtaa atgtacccgc gcgtttgatt
     2161 ggattcatcg actatgcaat agcattgaac atccttatgc caaacatcca cgccctcaat
     2221 ctcctcgata ctcataccaa agagcttttt aacaatcgcg aacaagcctt gcatcacttt
     2281 aggctgtgga aaataaggcc gtaattcctc ttgagataag gaatacaaat cctgtcttct
     2341 tttttcagaa agataagcaa cgtcccatgg gtttaccggg ctcagattcc atttatcctg
     2401 tgcaaacact tcaagttgcc taaattcagt tttacccttg tcacgagttc tgctgatcaa
     2461 atcatagata aattcagtga cttgattggt cgaggcagcc atcttggtag ccagggataa
     2521 ctcggcaaaa ttattaaacc ccaaaagctc ggctttttca tggcgcaggg acagaatttc
     2581 atcaatcagc ggagtattat caaaagttcc tgcattaggg ccttgatctg aagctctggt
     2641 aatgtaagct cgatacattt cttcacgtaa agccctgtct tcagcatgag taataacggc
     2701 ctgaaaacat gggtattcga gggttaaaac aaaaccatcg agccctttct catgcgccaa
     2761 ctcttttgcc gtatttaatg catgctcagg taatcctgcc acacgttctg cttcggtaat
     2821 atggatagtg taggcatggg ttgcgtccag cacattgttt tcgaatttgc tggataattc
     2881 ggccagtctt gattgaatgg cttcaaagcg ctttttattc gccttggata aagcaactcc
     2941 cgatagctca aaatcctgta tgctgtctgc aatgattttt ttctgggcag ggttaaggga
     3001 atgctgatct atcgatttaa tcgcctcata caagtcttga ttatgcccaa tcgcagcatc
     3061 ataggcagac agcaatggca gacacgcttc ataggattca cgtattgcct cagaatccat
     3121 caccgcatgc atatgtgcaa aaggagacca aaaacgctcc agttcatcag cgaggctatc
     3181 taaagggtaa attaaattat cccaggtata gtggtgattt tccttaagca acctgtcaat
     3241 ttcctcaaga tggtttttta ataaagcatc aagatgagat ttgaaatgtt caatttttat
     3301 atggctaaat tgtggcaatc caactgtggt cgacatgtta tacctttcca tcatgtaatt
     3361 ttttgaaagc ataacattca attgaattag tctctatact ttgccggcgc tgaaatggca
     3421 aagtacagta atgaattaaa aggtgaacgt tggaatcaat tcctctgcgt caagcacctt
     3481 ttctggcttg caagacaggt gcagactctc ttttttttca tttttggggg caaaaaagct
     3541 taatttattg ataacggaag gagcaggttt ttcaatgtta tcgacaggtc tgtattcatc
     3601 taacttgttg ttcaagtata atgaaacgcc ccatagcgct agcaaaagca ctgccccaat
     3661 caaggggatg acaggattta taatacccaa agcaggaact gataaagcca ttgcaatcac
     3721 aacagcgact gccccgccaa ccgataaaaa ggagcgcaaa atctcaaact taatgcgata
     3781 actgattgag tcttgatact ctttaattat ctttttctgg tcttcatttt cttcctggtc
     3841 gaagagttcg ccatattcct tttgcaactg ctgtaatcga ccaatctcta tgtaagcacg
     3901 caggcttgtg ttggcgacat caaaaacaaa cgcaaacaaa gacaaataaa cagagagagg
     3961 tgccaaagca cctaccagca cgaagcaatt gagaatcccg actgtaaacc aaacgctgtc
     4021 attggccagc tcaaaccacc gtcgttgcag gtgggcataa aatcgatcgg accaatccag
     4081 tgatttttct ttttcgccca tccattcccc tggaatggtg tgctttaaaa ttaaaaagag
     4141 attggtaaat aatctggggg caaaaaaaca ccagcccaaa taagccaaaa atggattagt
     4201 gtatttatca agcagcacga caaaatcacg aaatgccttt gaaccagtgt ctaaattatc
     4261 caaaagatta atgactcgtt tacttctgct gataaaaatt ctataccaat tggattgagc
     4321 agttttctca cgaatggcct gcgtcaacga taagcttgtc ttgagagatt ccggctccgg
     4381 attatccaag gcaaaagaat acccggacaa ttccgtcaat aattccctgt atatggcttt
     4441 atggcgacgt aaacgcacta cttcacgtgg aacaaccagg tagtgttgat aggtatattc
     4501 caaaagttca gccagtttga gtgcatcaat cagctgttcc ttgatttgct cgagagaact
     4561 gtaatcaggt gaggacaggt tatgccttaa gcgctgacac aatacgctgt attccacatt
     4621 gagaatggga tgaagacgag ctactttcga ataaaaatca gggcccaatt gcttcaggtg
     4681 cgtgttaaac gtccaattca aagaatcagt tttaacttgc gtgttcagct ctggaaatat
     4741 ttttaataaa tgactaataa tcggtgtcat gattgtttga aataaataat ccgcatatca
     4801 ttgtgtttct aatccagctt tgcaaaaatt tttgctcctg tcaccttatc tgggctgcaa
     4861 aaattaatca aactccatca ttatgtcaaa aattaggaaa aatttaaact taaatttaca
     4921 ttttgctcta atattgtcat tattttcaac taaaaatgaa tggtcttctt gccgatttat
     4981 gccttctgag cgacaatagt gcctcagtta aatgcaaaag agtaggcatg aacagtttta
     5041 cagaattagc taatgcagta cgcatgttaa gcatcgatgc agtaaaccag gctcaatcag
     5101 gccaccctgg catgcccttg ggtatggctg atattgccac cgttttatgg aaaaaatttc
     5161 taaaattcaa ccccaaaaac ccgcactggt ttaacagaga ccgttttgta ttatctaatg
     5221 gccatggttc catgcttctc tattctttac tgcatttgac cggatacaat ttggatatta
     5281 acgagcttaa aaatttccgc caattgcact caaaaacacc agggcatccg gaacatgggc
     5341 acactcccgg tgtcgaaacc acaacggggc cattaggtca aggtttggcc aattcagtcg
     5401 ggatggcatt ggcagagcgt gtactggcca gtcacttcaa ccacgatcac tataacctcg
     5461 ttgatcatta tacttatact tttgccggcg atggatgcct gatggaaggc atttctcatg
     5521 aagcgtgctc tctggctggc actcttggtt tgggaaaact cattgtattt tatgacgaca
     5581 atgggatatc tattgatggc aaggtagaat cctggtttac ggacgatacc acgtctcgct
     5641 ttaaagccta caactggcaa gtcataggcc ccatcgatgg tcacgacgca gctcaaattg
     5701 agcaagccat atcagcagca cgcgagaaca cgactcaacc ttcattaatt atctgtaaaa
     5761 ccgtcatagg actgggttcc tctgtagccg gtagcgaaaa agcgcacggc tctccattga
     5821 gtgcccaaga cataaataat gtccgtgaat tttttaattg gaaacatgct cccttcgaaa
     5881 ttccagattc gatatacaag caatgggatc atagagagca aggagaaaaa gaagagcaac
     5941 aatggctgca gctcctgcat gaataccggc aacaacatcc atcggaacac gatgagtttt
     6001 tgcgtcgtgc caatggcgac ctgcccgatg attggcaaga aatatcggct ggattcctaa
     6061 aacaatgcct gaacaatgag aaagccatag caacgcgtaa agcatcgcag caatgcatag
     6121 agtttttcgc gccaatatta ccggaaatgt ttggcggctc agcggatttg actggttcca
     6181 ataatactga ctggtcaggc agcaaagcca tcacggccca taatttttcc ggaaattacc
     6241 tttattatgg agtgagggaa tttgccatgg cagccatcat gaatggactt gctgttcatg
     6301 gaggattcat tccctacggc ggaacattcc ttgttttcgc agattatgca agaaatgcca
     6361 tccgtttaag cgctttaatg aaacaaagag tgatatacgt attgactcat gattctatcg
     6421 gtttagggga agatggcccg acacaccaac ctatagaaca tatcgctatg ttaagaatga
     6481 cccctggcat gacggcctgg cgccctgctg atttgatgga aaccgctgtt gcatggcaac
     6541 aatcgttaga acatcataat ggaccctctg cattgttact ctcaagacaa aatctgcccg
     6601 ctttagccca tagcgccgat gcggctgact tgattaagaa aggtggatat attattgcag
     6661 attgtgaagg gaagcctgat gctatcctga tagccactgg ttcagaagtt caactggcta
     6721 taaccgcagc agaaaaggca aaagcaagag gtctccaggt cagggtggta tccatgccct
     6781 gtgctgagcg attccttgcc cagagtgatg cttacaaaaa ccaggtatta cctggtcagg
     6841 ttcgcacacg tattgcgata gaagcagcta gcagtgccta ttggcaccaa tttgttggct
     6901 ttgacggcgc tgtcattggg ctggattgtt ttggggtatc agctcctgct acagatgctt
     6961 ttaattacct tggaattaca gtagaaaaaa ttatcaatac tctagataca ttaaccaaac
     7021 aaacaatggt gtaacccata ccccttctga agtttcaaga aggcctgtat tcagtataac
     7081 tgagtatgga ggttacccta actcggagaa gtagtatgac aatacgagtc gcgattaatg
     7141 gctatggccg cattggccgt tgcattttaa gatcgatttt tgaatacaac aggcaaaatg
     7201 agtttgaagt cgttgccatt aacgatttat ctggcatcga tgtgaccgct cacctgactc
     7261 gctacgattc aactcacggt ccttttcgct cagatgttag catagaagga aacatgctga
     7321 ttgttgatgg gcatggcatt caagtgattg ctgagcgtga tccagccaaa ctgccatgga
     7381 aacaattgga cattgatatc gtctttgaat gcaccggttt ctttaccagc agagatgcgg
     7441 caatgggtca tattaatgcc ggcgctaaaa aagtattaat atcagctccg gccaaaggcg
     7501 ttgatacgac agtggtctat ggagttaatc actcggtcat caaagcgtct gatgtcattg
     7561 tgtctaacgc ttcctgtaca acaaactgcc tggcacctgt ggttaaagtg ttaaatgatg
     7621 ccataggaat tgaatcagga ttagttaaca ctgtccatgc ttacacaaaa gatcaaatgc
     7681 ttttggatgg aagccataaa gatttacggc gcgcccgctc tgcaacgcag tccatcattc
     7741 caaccaaaac cggcgctgcc gcagccgttg gattggtttt acctgaactg gcagggaaat
     7801 tggacggatt tgcgatgcga gtaccagtat taaacgtatc cgttgttgat ttaactttcc
     7861 aggcgaaacg cgcaacttct gtggaagaag tcaatgacat catgcgcaag gctaaaagcc
     7921 gaattttaca aattaatgaa gatcctttag tttcttgtga cttcaatcac aaccccgctt
     7981 ctgctatttt cgacacaaca gaaaccaaag tgataggcaa tttggtcaaa gtagtagcct
     8041 ggtatgacaa tgaatggggt ttctctaacc gcatgattga taccgcacac caaatgatga
     8101 actgctaaag gataaccaat gaacctgatt aaaatgagcg acattgatct gtctggcaaa
     8161 agagttctga ttcgtgagga tttaaatgtc ccgatcaaag acggcatgat taccagtgat
     8221 caaagattgc aagcggccct gcctacaatc aaatccgctt tggacagtgg tgcagcagta
     8281 atcgtattgt ctcatcttgg gcgtccggaa gaaggcaaat acgaaaaaaa attctctttg
     8341 gaacctgttg ctgattattt aagggaaaac ctggaatatc ctgttcgctt cgttaaagat
     8401 tacctgaccg gcgtggatgt aaaccctggg gaattggtcg tatgcgaaaa tgtccgattt
     8461 aatcccggtg aaaaaggcaa tgacgaagct ttggcaaaaa aactggcgag cctttgtgat
     8521 gtctttgtca tggatgcttt tggcacagca cacagggcac aagcttctac ctatggtgtc
     8581 gctcaatatg ctccagttgc cgttgctggt cctctgttaa tcagggaact tgaagcgtta
     8641 aaccaggtac tcaaagcccc caaaaaacct atcgtcgcta ttgttggggg agccaaagtc
     8701 tcatccaagc tcagtttatt gaaacaatta gtgggtatgg tcgatgttct tatccctggt
     8761 ggaggtatcg ccaatacttt tctgaaagcc cagggatttg aaattggtat ttccctttac
     8821 gaacctgatt tattggatga ggctcgacat atccttattc tggccaaaga gaaaggttgc
     8881 caaattcctc tacccaccga cgttgtagtg ggcaaaacat ttagtgaaac ctgccctgct
     8941 ttcaacaaat ctctgtctaa tgtggctgcg gacgatatga tccttgatat tggaccagaa
     9001 accataagag attatgtaga tctcatccat gaagccaata ctatcatctg gaatggtccg
     9061 gttggcgtat tcgaattccc gcagtttgct tacggaacaa gagccattgc catagcgatt
     9121 gctgagagtg atgccttttc tattgctggt ggtggtgata ccttggcagc tgttgacctt
     9181 tatgacttga atcaacaaat ttcttatatt tccacaggag gtggggcatt tctggaatgc
     9241 ctggaaggaa aaaccttgcc tgctgttgct attttgcagg agcgcgcaaa acatgttaag
     9301 acgaactaag attgttgcca ctttaggccc agccagcaag gaacctgaaa ttctgcgatc
     9361 catgctggct gctggagtca acgtagtacg aattaatttt tctcatgccg attcctctgc
     9421 cctgcaatta attgcactgg ttcgtaaaat cgcagatgaa ttaaaccacc cggttgcagt
     9481 catggctgac ttacaagggc ctaaaataag agtcggccgt tttcaaaaca aatccatcac
     9541 tttaattgat ggacaaaatt ttacattgga ttgtatggct ccagatactc tgggtgatat
     9601 aaatggcgta tcagtggctt atcctaattt ggccaatgaa ctcagtatta gtgatcatct
     9661 tctgattaat gatgggttaa ttgaattgga agtcatagag atttccgggt caaaaattca
     9721 ttgcaaggta gtggaaggtg gagtactcac tgatttgaaa ggtttgaatc gaaaaggtgg
     9781 cggcttagca gccagaactc taacagaaaa agatagaaat gatttacgta cagcaattga
     9841 agcagaagtg gactacatca gtttatcttt tgtaaaagac gcagaggata tccggcaagc
     9901 aagagcgcta atgaaagact atggggccca aataacccct attatcgcta aaattgagcg
     9961 catggaagct ttggatcact taactgatat tattcgcgaa gcagacgcca ttatggtggc
    10021 cagaggagat ttaggcgttg aagtgggtgc tgctgaagtt cccgctattc aaaaacacat
    10081 catagaacaa acgcgacttt tagataaagt ggtgattacc gcaacacaaa tgatggaatc
    10141 catgataagc aatcctcagc ctacccgtgc cgaggtttct gatgtagcca acgcgattct
    10201 ggatggaact gatgctgtga tgctttcggc tgaaacagca agcggccttt ttccagtcaa
    10261 agtcattacc atggtcaata aaatatgttt aagcgctgag aaacatgcca gcttttttta
    10321 tcatagtgat ctggaaacct gtcattatca acgggctgac caggcaatcg ctatggctac
    10381 catgcatacc gccaatcatt ttcccattca ggccattatc actctgaccg aatccggaga
    10441 cacggcttta tgggtatccc gacaccacag caccgttcct atttttgcta tatcagccaa
    10501 taagagaacc attggtcgtt taagccttgt aaacaatgtc tttcctatct acattgattt
    10561 tcatcaattc aaccctgaag gattgaatca acaaatttta catgaattaa tcaaatcagg
    10621 gcaccttgaa aagagaggct atgttttgtt aactcgtgga acccaaattg gaatgcctgg
    10681 cggcaccaac tgcatggaaa ttatacctgt tgtctgagtt caagatatcc tgttaattaa
    10741 agcaaggttc tttcataaaa gacaaaaaat gattatttta atgacacttt aatcaaatta
    10801 tcacctctat gctactatta agcatagagg tgatcaaaat gccggataaa aaatcatcag
    10861 aaactctgtt atcgcagcaa ataaatgaag taagaggttt gctgctgcaa gcccttaaaa
    10921 ctcaattaga tgatctggta gctgaaaaac tcattccaca accaagtggc gttccttttg
    10981 ttgattttga gaaagatcca ccacaaattg cgactctaaa aaaagtaatt aattgtcttt
    11041 atcatgcaga ggaagcgttt aaaagctggg aaagcattga caccagtacg ctattgggta
    11101 aagccaaagc agcaccaaaa ttaattcacg cgctaaccca gatatacaaa tccttgggct
    11161 tacttgatga ggcaagccct gaaataagaa gtgtcatagc agacaattac catttacttg
    11221 aacctctatt tactcaagcg tatgccatcg tgcaagagtc tggctggtta tcagaattca
    11281 tggaaatgga tgtgaccgat aaagcatcca aagtgatctt ccaagggatg gatttattag
    11341 gtcccgatgt cgataaatgg catgatacgc atcctcttgt tgacgcattt tcaaaaatat
    11401 caaaactggt agaaaccata tccacactac aagaaaagga catgccggga aaagagaaag
    11461 aacaagcggt agaattgatt cgcgccttac ttgatgacct ggacaataac ccttttatca
    11521 gcaaattaag tatcagtgat tttgaagact ccaaagccat aaaggattta ttggaatggt
    11581 ttaagaacat acaagacgat ggttttgatt ttagcaagaa atccattaag caatatgttt
    11641 cctgggcaaa tcactattta cccacattaa tcgccttcgc tgatcaactg gaacggcaaa
    11701 actatttaaa accaggcagc ttatccgcca atttgtgttc tcagatagaa catttgggta
    11761 aacaagtgaa tgacatttta tctgaacctg gttttggtgt tactgagcga gttgtaacaa
    11821 tagactcatt gcagggacaa agagagcaaa aaattcataa cgctcaggtc aaaagtgtgc
    11881 aagttatcat ggctactgaa aaacaactag cggccactgc tgatttttat cagatcctgg
    11941 aagcctataa gggcatgaat ctttcacaaa taacagaacc ggatcggaga gtgctaagac
    12001 aattatatcc ccaaatacaa acagcgctgg cgcatgccag tttggatctt gaaaatcagt
    12061 taacctctgt tttaaacact accggccctg aaaaaacccc agctaaacct aaatcatggt
    12121 gggaattagc caaagatggt ttaggttatg tagccagtta tgtggtcaat tacaacgttg
    12181 ataaaatcct gggaaccagg gaatctgttg atcgttttct gtcaagccaa attgcgtccg
    12241 agcaatttaa gatcctgatt gctgaaaaag cccgtgaaaa attaagaacc ggatcagata
    12301 aaataaaaga aactgcactg caatatcaag cggaagctcg tctcaatgct ataaaatcaa
    12361 ccctgagaaa acaaccctct cagatggagg ctcccccatc cgaatttgtt gcagtgaaac
    12421 catcgaaact ggtcaatctt cgtggaacac ttacagccat tcaggaaatg caattatcga
    12481 ctacagtaaa tggcacaaga acaagtgtcg atactctgat tcatcgccat ttgccagaaa
    12541 aatatcaagc ctgtttttca actcttccct atcaaatcaa tgaaaatgat cctgagttgg
    12601 ttaaaaaaat taaaaccgtt gaaaataatt tgtttcaatt agaaaaagcg cttaaagatt
    12661 ttgaaaaaat agacttgagc tatgggatta tgattcgatt gcattatttt atcgcaatag
    12721 cttctgcagc ctccaaacta aaatcaagtg ttaaagaact tccccctgag ggtcaaaaag
    12781 cgctagcccc tctattacaa caaattatga cattaggctt atccttctca gacaccaatt
    12841 atacagctaa tgatttgtcc gccttgcaac aattaaaaat ggaaggcgag gtgagaccca
    12901 aaagtccatc cgtagcatct ccagatacaa taggaacgtt acctgaagaa actggaaaga
    12961 gaactgtaaa aaaaccaaaa gaaaaaacga ctgaattatt ggattatgct gacaaaatca
    13021 gtaatgctcg agcatcgcta ttaaataagt ttaaaaacac cctgtctccg gcactagccg
    13081 acagtttaaa tccacaaaaa aatggcgttc catttgttca tcttgatcaa gacccgccac
    13141 aaatcgcggc aataaaaaaa atcatcaaca gtatgtatca tgcggagtgc gctcttaaga
    13201 cctggcatgg catagatacc agcactacat taggcaaggt tgctgcagca catcaaggcg
    13261 tagcggcatt atctcaagtg tacaaatcat ttacattgtt caccgaggat ttctcagaca
    13321 ttcacaattt aattcgtgag aaccacgatt taatagcgcc tgtgattatc gaagcaaaca
    13381 agctggttaa tcagtatgga tgggcacgtc aattcagtga gcttgatata gcgcaacaac
    13441 tgggaagcat tttaggtcag ggaattaata cagttcaacc taaaacggat gaaataacgc
    13501 aaaccgcttc tctggttaaa ttattttcag aaattccaag gttattgaac aatatttcaa
    13561 gttcattgga tcaaaaatct gagaaatcaa ttgatgagtt aaaaatcagc aacaaaaaaa
    13621 ttgaagccat aggcgcagta tttgaattgt tttttgaaga aaatgcccac ttaatgagca
    13681 tgttcaaagg attcagcgct atctctggtc ttatcgaatt aaataaaaaa attcaacttg
    13741 aaggaaccaa cctgcaggaa gtcactattc gacaatacca gtcatggtta aaagaaggtt
    13801 atcccaattt gatgatgatt cttgatgaaa ttgagactcg tcactactta acccctggct
    13861 ttttatcagc acctattgcc ctggaattag accaaattaa cgacaagctt aatgaaatta
    13921 ttgaaaccaa acctgatttt aaattacaaa aaattcctct ttcattctat atgggagata
    13981 aacgcataga gcgcttaatg actaaaaaaa cggaacactg cctcgcagtg ttgcaaatag
    14041 aagagcaaaa aaaagcagtg aacacttttt tcgggatact taaacactat tcagatcaac
    14101 cgctgtcaaa cataaagcca aatgagctcg ctcaattgcg tcttgctttt gtagagattc
    14161 aatccgcaat ggcaaatagt aatttggaat taagcaatga tttcgttgct gctttaaacc
    14221 aacttgaaac ctccccacct gaaaaaacaa aaaaaatcca ggtgacagtc tctcaattgt
    14281 taaaggtgca gcctgcagtt gaaacctatt taaacgagca caaaaaatcc tctgaattaa
    14341 aaatagcagt cattgacaaa gccatcgagc acgttaaaga caaatcgttt gagctgaaat
    14401 ctggtgcatc aaaagaaatc aatatccctc agctgcgcaa aacattttta gaaactgaaa
    14461 gcaagaagcc tgttgtaggt cctggggaat taaaaccaac atcagtctca tctcttaaca
    14521 ccgttcgtgg taatttggct tacatccaag aattaaaaat ctcatccacc gtaggcatga
    14581 tgcgagaaca gttttctgct cttaccagag ataatttctc caatcgagtg caaaaatatt
    14641 taatcaaacc tcaagacaag ccattgcata tgataaatga caacgatccg ctcatggtaa
    14701 ggcaaattaa agaagtcgaa aacggtttat atcacttgga aaccgcttta aaacaatttg
    14761 aacaaatgaa aaagagcgac agcctggtat cacaaaccaa agcactgcta gaaatagcaa
    14821 atcatgctct ccaattaaaa atggcggtgg aacgattttc tcctgagtta aaagagcatt
    14881 atggtccatt agttcggact gtattgagtt tcagtaataa gattcaatcg atcaattaca
    14941 acaaagaaga ttgggccgat cttcagttta ttcttaatag ggcccataaa gaattgttaa
    15001 aaagaaaaac tccaagagac catcgtattg aaaaagcatt ttttgaagct ggagttcgtg
    15061 aagtacaaat cgatcgtgat gaatcggtag gaaaaaaagc tgcaaaactg ggggtaaaat
    15121 acgcgcacct ggcctcccct cagttagaga aagcaagagc ctatttaagt tccagataca
    15181 aaaatgcgtt tggtcaacaa cccaaggtta ttcgttcgtt tacccgagaa caattagcga
    15241 atgaagaatt tatggacgct gaaattagca gattgaaaga aacattagaa gagaagtatg
    15301 gttttaatat tgcaacgatc aaagttcttc ttgatttatt agaacaaatt caacgggctg
    15361 gggctcaggc agctgaggta gctggcatgg tcaatacctt ggttacttat gacttcccca
    15421 agataaaaga aaatgcgtat agagatttac ttacgaaatt atctcaggaa gaagactacc
    15481 tgaacttaaa accaggcaca ttaattaatc cagccatggc tgcagtcaac caattattct
    15541 tatctgctgc cctggaatta gacatgcctt ttagtaaaaa gttatctatt ctggacgata
    15601 ccacttatgt caatattctc atcagacaaa ccgaaagcga tctgcaaaca ttaaaggaaa
    15661 agctatccga agatccctcg aatcatgaaa tagcatttga aattgagatt aagaacgata
    15721 aattagcttt cctcaggcaa cagatggtgt tgcttgaaga taaaaacccc aatcaaacca
    15781 gaaatgtctt aatggacatg cagttcgaag tgtcattacg taatcacctt gtttatacaa
    15841 cgcttaaagc gccgattgcc caggaatatg aacaaattgc catagaatat tacaaaaaaa
    15901 acaaaaatcg ctttctatat gccgatgact gttcaaaaga gttgctgcaa ggattgaaag
    15961 aatttgaaaa aaaacatttg cctgatcatt tgatagtttt tgaagcctac gacaggctcc
    16021 gcctattcag tgcaaaactc ccccccaaaa atcaggatgt caaagagtat attgagaaca
    16081 tcaatagaga attacaaaat agagacatcc caataaggca acgtgcttta aaagttaaat
    16141 tattacccaa tgacactgtc tttattgaaa aattaatttc agctgataaa ggcactcatt
    16201 tcctgagaaa attcatgcag tttattacaa tcattacagc aagcatcacc gaagcaataa
    16261 aaaccggggg cagtttggtt tatatttatc gccaaaaaca aatggaacaa tcaataaaga
    16321 atatagaaaa atcaataagc tttaaagaag agctccatac cataaaagat cctgatggcc
    16381 cttcaccacg caaggaagaa ggacaagaag gtgaaatcga atttaccaaa ttgtaaggct
    16441 caataggcta tattggtaga aaatcattct acactcagga cttaccccgt gaacagtgcg
    16501 taaacagcaa tggcaaaagt aatcatgtta aatgcctaag gttgctttaa aatcataaag
    16561 taagcatcaa cataatcagg taaatctcga gtagccctgt acaaagcgtc ctttcctgca
    16621 aagcttcctg ccaaataatc aatttgatat aaattattat taaaagcgcc cccagtatca
    16681 gccaggatgc ccattttagt cattgggcgt cctgatttgt cttgatactg aatcatcagt
    16741 aatttcccta aaccaaactc agccaaatca gccgcaaagg tcaccttggg gttaactgta
    16801 attttgtatt cagcgtcctt accataacct ttaattccat caacttcctt gaaataccaa
    16861 aatcgctcct gctgataggg ggttttagtt cgatcataag taatattatt actacgatgg
    16921 acattaaata ttttcttacc cgcagtcggt ccaaaatccg cgacaacagt accttgcagt
    16981 aaagccgcct ccaaatcatc tcgacttaaa taagccaatt cgctaaccgg ttgattttta
    17041 agagcaccgg ataatatcgc ttgcttacca tatttgaaac ggatcagtcc tggtttggca
    17101 tttgcttcat ccagagtcaa attctgctcg tcttcaggca aagcatacaa aggatagggt
    17161 ttcgcggagc ttggtttggg actggcacct gccaaatgca caaaatattt agtcatcaat
    17221 atcttatctt tgggtatatt ttgtaataat ggtttattgg cagataattg tttcgcttgc
    17281 cttaagtcag gataccatcg aataaaatcg aagtgctgtt ttacaaaatc cgggttattt
    17341 aatttttttt ggttttgaca aataaattcc aaggtttttt tcactctgtc taaagaaatc
    17401 ttaatgactt ttccatcgtg aatcacttgg ggatcataat ttttgccttt atttaagtag
    17461 gctaaagtat gctttgctgt actgcataat tcagttccct taatgtcata tactccattc
    17521 aaggcaggtt gggatggaaa gaattttggc gcagcccata gtggagaaat aaacagtact
    17581 gctgaaaata atgtcgctat tcgcatttac tctgttgttt cctttttatc ttctgctttt
    17641 cgagacaatc ctaatttttc ttttaaacgg gctacagcat ccggatcata ttgctttggt
    17701 gctttatgtt tgactactac ggatggtgac ttggcggatt gcattgccac attttgtgag
    17761 gcataggtag agcttctgag aggatatatt ggcaaaagag gctcaggatg agagtcaaac
    17821 gaattcattg gcttaacgct ggaaactgta cttggctgat tatttacatg cgaatgatta
    17881 gccgcttttg cattcacttg tttgcgagca tttttaacac tcttttccac gcgtttttta
    17941 attttcatgg aagcattctc cgcttcttcc tcagtaacct ccgctactgg atttccgtgc
    18001 aaatcgatac ggacttcacg agctttcagg caagccagat aatcaagacg acgggtaaaa
    18061 agaacaacag cttcccttaa tttactttta gaaattccaa cttgctctgc tttttctgca
    18121 tgctgcaata tatcagacat aataccaatc tttaatggcc gaatacgcaa agaattatcg
    18181 aatgcctcag ggaaattagc cgcaagccat aataatgcgt cagatcgcgc gcgcttggat
    18241 tgattttttt gtgctttatt gatgacagcg gttcgtgggt gcagcgcctg ctttctcatt
    18301 ctggtaccct tattgtgttt agtctattgg catgaaaatt atcaaaatca tttgatttgc
    18361 aaaggtctgt tgaccttgaa tgtatgatag tcatatcttg aagtcagatg gtgtaaatta
    18421 tgctattagt tactcgagaa atgagcgcac aatgtacaat gtttattggt tgtttgcaag
    18481 ccttctttat tatttccatt aagccaaatg tcttttggtt tgtatccaat tagcaaaata
    18541 cattatgata tgtctataat taacttaagt cagatagaat agcgcagagg atgcttgata
    18601 gattgctaaa taaaggaacc ttatcgctca ataatgtatt aatggtgatt acaaaatgag
    18661 gataatactt tcaattcttg ctatattcat gccgtggttg gttttattga ttaaagacaa
    18721 tccaggcgga gctgtcgttg ccttgattat gcaagcgaca ctaattggtt ggccttttgc
    18781 ttcaatctgg gcatggcggg cagtcaaccc acttactccg gcgtctcaat ccaaacagta
    18841 agtatctcaa tcctgttttt attcaacata taactaaatc aggacttaag aaaaatgcca
    18901 agtctatgca aaaaatgttt ttaatgaggg agttcagata ggctcaatga ccgaattaaa
    18961 atttttttta taaagagatt gggatttttc gtaaatcatg taaataattc ggagtgataa
    19021 atgcgaacac gtgtcattgt taatggtgcc aatggaaaaa tggggatact agcctgtgaa
    19081 acattggaaa accatgaaca gtttgaagta gtagccaaac taagcagaca ggacaatctt
    19141 gggcaaagta tactggacac caaagcacaa atagttgttg atttaacaag agcagactgt
    19201 gtctatgaaa actctttaac cattattaat cacggcgctc gaccggttat tggaacatcg
    19261 gggttggttg aaacacaaat cgatgagctc accaaactgt gtgaaataaa acaaataggt
    19321 ggaattattg cacctaattt ttctctcggc gccattttaa tgatgatgct cgccacgaaa
    19381 gcttctgaat atttttcaga ggttgaaatc attgaaggcc atcatcaaca aaaattggat
    19441 gcaccatcag ggactgcttt aaaaactgca gaaatgatag ctgcggcgcg caaaaaaccc
    19501 aaaaacaaac ttccattaaa agaattaacc cctggggccc gaggcggctc tcatcatgat
    19561 attaatattc attcccttag actgcctgga ttgcttgctc ggcaagaagt cctctttggc
    19621 aatataggcg agacactctc cattacacat aacagtatag atcgccactg ttttatgccg
    19681 ggaattgtgc tggcctgtca aaaagtattg aatttaacta atcttgttta tggtctggaa
    19741 catttgcttt aaattgcaaa atccaggtcg acccgtatta ctatcgggca tacgggtctc
    19801 cttttcgcat ttcacccctt gatatggaac gtttcccaac tcaatttttt aaacattaca
    19861 tataaaccaa tccgctgttt tctcttttgg aactctcaaa caaagaagac cattctaaaa
    19921 ttgcttcttc tgccaatgtt tgtaatgtgc tttctgatga caggggttgt aataatttcc
    19981 taatacgatc atagactgaa ttggttgctg tagttcttga tgaatcgctt tctggccgat
    20041 ttaaaacggc agcaaatatt tttccaatac gaagaacatt atcattgccc gagttttcat
    20101 catgcattac ttcacgaata ttgagtattc cagtaggtgt tgagctcaca tctttatcag
    20161 agaaccaacg taatttccaa gagtctttgc ttgatttttg aaatctgcgc tcctcattga
    20221 ttaaagcgct tagcacatca taaagcattg tacacatttt ttcgcccagc tgtttggcaa
    20281 ttaacaagca atgcaattcg ttttctggta gaaaaaagga acgcaaatcc ttgctaatat
    20341 tcttcacttc attatcagtt gctaatcggt catcataggc taatgctttc tcagtgccca
    20401 agcgctcgtt aatggcctca gcgatatcgt ttggccagta ccaatcaggc gttttgattc
    20461 cctctgaacc tggcgcattt tgccctgcaa gctcatgttg atgtctggag atataaagat
    20521 ctacaacaat attgatgaaa ttgactcgtt cttgtttttc tttgggaatt ccaaatttag
    20581 gccaatttcc atattttgct ttatacaaaa tcatcgcatc agtatgcaat aactctactg
    20641 cttttctgtc tttaccactg acacaggagc catagctata gccgcccatg ttgagaataa
    20701 tcagttcttc caaagagcta agaaataatt ccctgccatc gtaatcccaa aatgttgcag
    20761 agcctaaagg cgactctaaa acggccttat aatcatcgat tagcgcctgc aatccaagct
    20821 tggaagacac atatttctgc gctgctttta ataaaaactc gctgtctgta tccgttgcct
    20881 gagtataata ataacgcttg gcaatattaa aaggatgatt atgctggaat atttcatgcc
    20941 tgcgtttgct ccgggtgacc gcttctcggg caattttata caactccaga tccggaggta
    21001 attccggtaa aaagtctgta accaccgttg gaatataatc tactgcatgg atagggctaa
    21061 ttaatgtttg caataacaaa ggctgctgag gtttggcaaa ttccatcacc ttaaccaggt
    21121 tggagtcaac atgacgctgc tggacatcct caggaaattt taacgaatca cgactggcaa
    21181 catggcttga gcgatacctc ttgtcataaa ataaagtttc tttaccctca ccatcgatta
    21241 aatataaact gtgcgcacca taattggctg gagcaggcaa agttcggtgc cggcttgata
    21301 aatatgatac gacatcctca accttttctg catttttgag agcatattcc aaaaagtatt
    21361 gttgttgctc ggataaaacc caataccact gtggaagctt gggcactagg ctcaaagtcg
    21421 atgcgtattg atcattcctg gcttgctcca ccataaattt tttaaactct tttaagctgg
    21481 atttaatctc atgtggagta gcagacaaga cgcgaatcat ggcttgctga gcagggctta
    21541 attcattgaa ccaggaagga taaggcggtg agttggtatg tatttgattt aattccgtgg
    21601 tcaaattcag tgaccttttt gcaatgtctc cccaattgcc aaaaaatgta ttaaagtgct
    21661 gcagcgcttt tttaggatca acagaactta aattcagatt acaataataa gcttgttgat
    21721 agggcggcag atttttaaac caggcaggtg ttttaggaaa attatattct tttaattgtt
    21781 ttaattcagt taaccattgt tcataaacgg gagacaggga ttcatcatat tgcaaaacat
    21841 actttgtctc actgcccatt tcaataggca ttatggtagc gatatcgcta cgaccccggc
    21901 tcattaagac atactgctct gcttcattaa ggctggctat cgcctctttg actgccttac
    21961 ctttaggcca tttccacgcg atggcaatag cctcaaccaa tttggccgta aatcgttcga
    22021 gctcaagatg caactcttct aatgtcagtt tttccaggtc acatgaagcc ttgcattctg
    22081 cggcataagt acttaaggtt gcaaccgtca gagaagtatg ttttggatcg agggggtaag
    22141 cttttaagat aaatgaaaag gcatcatgaa ggctgtctat ccggcgagcg atgcttttac
    22201 gataatctgg ctgctctttg aaatactcta ctttgtaacc aggtagaagt cgctctagtg
    22261 tatcaaatac ctgaggagat aatattgtat tttccaaaac acgtaaaatt ataccttttc
    22321 ccatagctta attcccttac tgccttaatt tcctcattat tgtataaata attcaaagtg
    22381 acaatgtttt gaattaacaa ttgaattata tatttaccct aagatatcag gaagttaatt
    22441 gccttaatag cattacttca tgctaaacta gtttttttgg aggaatagta aaaggatagc
    22501 tatgaaaagt atagaggatt ttacaggttt ttcagaatcc gggattgatg atgccattca
    22561 aaatgcttta aaaaaagcag gtaatcccac tcattttgaa gtcgttgaaa cactaggttc
    22621 acaagatagc caatcagatc gacgatatcg ggtaacgctt aaaacactga gtgattaatc
    22681 attaagtaac ggcaatcatt ggctagaaat aaatgacaaa aatttaatgt caattctgtt
    22741 tctataacta ttatctagct gctgcctttc aatacaagga gtttaaaaat gggatacaat
    22801 gtgcctcact acattggtgg acaacttatt aatgaaacgg atactgaagc tcaagccatt
    22861 tataatcctg ctttgggtga aattattggc caagtccatt ttgcgtcaaa ggcaacctgt
    22921 gatagagctg tggctattgc caaagcggca ggaaacgaat ggtcgcaaac ccctgctata
    22981 aaaagagcgc gagttttatt ccgatttaga gagctgctgg aaaaatacca aatggatctg
    23041 gcacgaatag tcactcgtga gcacggcaaa acgctggatg atgcgaaagg atcggtggcc
    23101 agaggcattg aggtagtgga gctgcactgt gggctcgtca acgagctaaa gggagatttt
    23161 tctgcggaag tggccagcca tattgattgc catacttttc gacaaccatt gggagtatgt
    23221 gccggagtat ctccatttaa ctttcctgtc atggtaccca tttggatgat gattcctgcc
    23281 attgcttgtg gaaatacctt tattcttaaa ccctccgagc aaaatccctc tgcgcctgtc
    23341 cgtttattgg agttgttaag tgatgccggt ttgcctccgg gcgttgccaa ttgtatccag
    23401 ggtaataaaa cgactgtcga gcatttactg actcatccgg atatagctgc ctttactgca
    23461 gtcgcctcca ctcctgttgc cgaatacatc tataccactg cagcagccta tggaaagcgg
    23521 gcacatactt ttggcggagc taaaaatcac tgtgtggtta tgcctgatgc cgatttagac
    23581 caggcagcta atgctatcgt cggagcagcc tatggttcgg caggagaacg ttgtatggct
    23641 ttatctgtcg ttgtaacggt tggtcaacat accgctgatg ccttgatcaa taaaatttcc
    23701 cccctcattc gagccattcg cgtagacgcc ggagatgttg aaggcactga tatgggtcct
    23761 cttgtcagcc aggctcatcg acaaagagtt ttggccgcta ttgaaaaagg ggttaatgaa
    23821 ggtgcccgct tgattatcga tggcagaacc tttcaacatc ctcaataccc acagggtttt
    23881 tacctgggac cctgcttgtt tgatgaagta accgaagaaa tgtctgtcta tcaaaatgaa
    23941 atttttggac ctgtactggt tatcgtgcga gtcaaccatt ttgaagaggc tcttgcttta
    24001 gtcaataaac atcaatacgg aaatggcact gctattttca ctcgggatgg ctttacagca
    24061 cgtgagtata gccagcgagt tcaagcaggc ttggtcggca ttaatattcc aatacccgtt
    24121 cccattgcca accatccttt cggaggctgg aagcgctctg tgtttggaga taccaacatg
    24181 cacggagaag aaagtataca tttctatact cgacgcaaaa cggttaccag caaatggcca
    24241 ataaccgaat taaaggatag cagctttgcc atgcccactc atgattaatc atgcttatta
    24301 cggagagata tatggctaat attggatttg tgggattggg tcatatgggt tttccaatgg
    24361 ccattaacct tttgaaagct ggtcatagtg tcatgggcta tgatttgcaa caatcagcac
    24421 ttgagcattt tgcagaacaa ggaggagcta ttgctcataa cttgcaggaa ttggcaaaag
    24481 acaaagatgt cattgttact atgttacaaa ccggacagca agttttacat gtatgccttg
    24541 gtgtcgatgg tatattcagt cagttaaatg ctggctcatt gtacatcgat tgctctacaa
    24601 ttgatgtaaa aagctctcga gaaactcatc aaatagcgaa agaaaatcat cttctggtcg
    24661 tcgatgctcc cgtctctggc ggcgtagccg gggccactgc agctacctta acattcatgg
    24721 tcggtggtga agcggaggct tataatgcgg cgcatcccat tctctctgcc atgggaaaaa
    24781 aaatcatcca tacaggcggt gcaggcagtg ggcaagcggc caaaatttgc aataacatga
    24841 tattgggtac cacgatgatc gccatatcag aagcgttcat tctggcgaaa gaactagggc
    24901 tgtctgatgg aaaattattt gaggtggtaa acaattcatc gggtcaatgt tgggctatga
    24961 gcaaatatgt ccctgtacca ggaatattgg aaaatgttcc tgcaaataat gattataagc
    25021 cagggtttgc agcaaacatg atgttgaagg atttgttatt gagtcaaaat actgcgaaat
    25081 cagtacacat tgaaacaccc cttggcgcca aaacaactga aatttatcaa caatttgtcg
    25141 atcaaggcct tggtgatatc gatttttcag ccattattaa acacattggc aaaacacaag
    25201 aggtgtagca tgcttcccgt tcatctaaag catcctcatt taacacctac agaagaatct
    25261 ctatctgaat tttggtctga aattgccgca caaaccattg actggataca accttggagt
    25321 attaccctgc agggagggtt acacaaaggc gatgtgaaat ggtttcaagg cggcttactc
    25381 aatgttagcg ctaattgtct tgataggcat ttaccccata aagcgaatca aacggctatc
    25441 atttgggaag gtgatgatga aaaccaaaat aaaaccctga cttttgcgca gctttattct
    25501 gaagtatgta aaatgagcaa tgtgttaaaa agcctaaatg ttcgcaaagg tgatacagta
    25561 ggcatttatt tgcccatgat tcctgaagca gccattgcca tgttggcttg cgctcgtata
    25621 ggggctattc ataccgttgt gtttgccggg ttttcggccc atgcattgca gcagcgatta
    25681 acagcctctt cctgtaaatg tttaatcact gtggatgcgt ttcaacgcgg tggaaaaact
    25741 atccctttaa agaaacaggc tgacgaagcc agcgtggact taaatatcac caagctggtt
    25801 gttaaaaatt caaatgttcc aatggcacta aataagaaca aagaacattg gtggcatgag
    25861 ctaaaacaaa ccgtatcgga tcaatgcacg ccggaaccaa tgaacgccga agatcccttg
    25921 tttattctct atacttccgg aagcacggga cagccgaaag gagtggtaca taccacaggc
    25981 ggctatttag tgcaggctgc ttatacgcat caactgatat ttgcttgtca ggataatgaa
    26041 gtattttggt gtactgctga tgtcggttgg attacagggc atagttatgt cgtctatggc
    26101 cctctctgca atggcattac cacattgatg tttgaaggaa tacccacgtg gcctgacgca
    26161 gcaagaaact ggcgcatcat tgataagcac caagtgaatg tattttacac agctcccaca
    26221 gccatccgct ccctgatgag agctggtgat caatggctaa actcaagctc aagaaattct
    26281 cttcgtttac tgggttcagt gggtgagcca attaacccgg aagcgtggaa ttggtatcac
    26341 caaaaagttg gacaagggaa atgcccgata gtggatacat ggtggcaaac tgaaactgga
    26401 gccattatga ttagtcccag agccagtgat gaagtgatca aaccaggctc tgctcgtaaa
    26461 cctatcccgg ggatcatacc actcctgcta aatgagcaag gacatgaaat taatggctct
    26521 ggagaaggat ttttagctat caaataccca tggccttcta tggcaagaac cattgcagga
    26581 gatcaccaaa ggtactgtaa cacttattta tccaacggct attacatcac tggtgatgga
    26641 gccaaacgag atgaggatgg agattattgg attacaggcc gaattgatga cgtacttaat
    26701 gtttccggcc atcgattggg tactgctgaa attgaatcag cattagtcag ccatcccaag
    26761 gttgctgaag ctggcgttgt aggtattccc catgatttaa aaggtcaagg aatatttgcc
    26821 tatgtcattc tgaaacaggg aaataagccg gatgccaagc tccaaactga attgatggag
    26881 cgagtcaagg atgaaatcag tgctatcgcc aaaccagatg tcattcagtt tgtaaatgat
    26941 ttgccgaaaa cccgttcggg taaaattatg cgccgaatac taagaaaaat tgcttgtaaa
    27001 gaagtgtcgc acattgatga gctgggtgat ttaacaactc ttgctaaccc tcaggtcgtt
    27061 gaagaactca tgaaaaatgt tttgaggcag taaaaagaac tgtcggactt cacaaagttt
    27121 attctgttag caaacaaata aactatgtga agcctgacaa ccatttcaaa ccatatcaaa
    27181 tcaatttgat caggctctga cagcaagcgg ttgcattgac actgaaaaat ctttgacaat
    27241 tttttcagat tcggtgggta caggaaataa tcgccatctc cccgcgtata aattggctcc
    27301 acccaaagtg cataaagtga ctaaagcact cgctaaatca gcaaacactt gtttccagcc
    27361 acgatgtatt tctaaaacag gtatgactgc attaatggca cgggcgcaag cttgatgaaa
    27421 atccttgcat ttcacattct tgggcagctg agaatttttg aaacgctctt cggccattaa
    27481 taaattatta taaaatgctc tcgctatggg cgccgcgcgt ttataatttt tatccccaac
    27541 tgctttggct tccagtgttt taaccatact ttcaataatt tttaagtggt cggaaaattt
    27601 aatttcggca agaatattca aagtttcttt tgttgcagcg atttggtcag taatacgctt
    27661 gctcaccgca tcagcttgcc tgttaatttc acgaacttta tctgtcagag ccattttgat
    27721 atcactatga tactcaccag ataaaagttg aagctgttcc tgagccttaa gcaaatctgg
    27781 tttgttgccc accaaatttc gaaccgattg aattaaatcc tgttttctgg taatcacttc
    27841 ttcaactgtt tggcatctgt taaaacttat cggaaattcc ctgatttgtt tttcatagct
    27901 ttttactact tcttcagcat ttattttaat gatcaattga tcagctgttt catttatttc
    27961 ttgttttttc gcttgcagtg ctcttactat ggatggttgc aagtcattac aatccagaac
    28021 tttttcagca cctaacaaag cttgattagg tttaaccaga ttatcaagtt gctgtaataa
    28081 attattacgc tgccattcta ctgcggaaga agtgcttata tcattaaaac gaattacaaa
    28141 attatcaatt cttttttcaa cagaatcgat aaccttttgt gcctcatcaa gaagctgttt
    28201 ttgcaataat tcagcccgca aattaatctt ttcaacctga gcaaaataga ccatttttat
    28261 tttgggattg gcgattaagc gttcgcccaa aagatcttca gccttgacca gtgtgtgaag
    28321 ccttttgttt aacagaatac gttgggattc aatagctact atggtacccg caccattaaa
    28381 gtcgattggg aagtctttta ttcgcctgac atgctcatct atatccgctt catacttctt
    28441 gagaaaatct aagcgtcgat tagctgcaac gtctatcatt cggagcttac gttctaaagc
    28501 cttctggact tccggatgtc catcaggtaa atccagtatt tgcaacgcat tttgcaattc
    28561 gccagagtga actattttct taagttggct tcttaactct tctgcattaa gattaaggtg
    28621 tgcaacggtt ttaaagccct caaaactaaa tgaaaaagaa ttgatgcgtt gaacgatatc
    28681 tgaaatcact cttttagcat cttcgactcg ttgttgcctt aatactttat caatttcagg
    28741 aatttgtatc gctggctgtg ggttggataa ggactcaaac catgatttga attcgcctga
    28801 atcttttaaa gcgatttttt ttgcgtattc ttctaaagat atgccaaata gaacattaat
    28861 tttttgagat gcgaaaacaa gtaaagcttt tgccacttca ggactaattt ggcttaatct
    28921 gaacaagttt tcaaataatt catccggatc tttaaacgtt gcgctggtaa gaacgatatc
    28981 aaaaaactcc tcatcaattg gcgatgtgtc ttggcttttt aagtactcaa tcaataaatc
    29041 cggtaaagtt tttagtgtga gatagttggc gatcgctttt gcaaaaagag cttttggtgg
    29101 aaataaagtc tctattgatt ttttggagaa aacattggct ataaacaaag ctcttaatcc
    29161 tgatgctctt gttaaattag aagatgctcc accaaagtaa aagttaatta caccggattc
    29221 aatttcttgt ttggcttttt caggtgtgta agcaaattga ttgatttttt gctgtattgc
    29281 agacttcact attaaatgga gaaaagcctc gctattaaaa tttattcctg cttggggact
    29341 taatttgttt tcttcgactc ggcttgacaa ggctaactct gccaaaacct cacctagtaa
    29401 ttcttgagat gtttcttcac gcagaaccag ttcaagcaaa tccattgcag ggatatgggt
    29461 taaaaataat tttaggaatt cttttttaga accatccgca ttggctatgc aagcaattaa
    29521 tgttttcttt tcttccactg acaattttag gaaaagtttt tcaagataag ggatttgttt
    29581 atgcaaacta atgcaagaag aaataatttt gttgtcagtt agaagattca tttcctccaa
    29641 agatatcagc cttttctgca gcccagaatt agaaaaaaca tacctcccct catcgttgga
    29701 aagtgtcaat aatttttttg tcaaactggc tctatggata ctggtattaa aaaggcaaaa
    29761 tcggcagttt cgcttgttga gatcgtctaa cttgtaagtt tctgttttag aattatcgtg
    29821 cggattgatt aaaacaaaat cataatctcc atgaccttta ggaataattt tgtctattct
    29881 taaagcatga cgtccatgaa attttccaaa gccatcgact tttccataag acatactaat
    29941 ataaacaggt tcatcaggat tcattagttt caatttgatg atatcgtcaa gctttgaata
    30001 tggaatgtct tccgcttcaa taccaaagaa tttgcccaaa aaagcagtgg aagtaaaacc
    30061 ggcaattctg tccggtatat catgagcaat aacgcttgcc aaaggattag tattactcca
    30121 atctcccgca taataatagg aaaccaatcg ttccaaaatt tttattgcca gcgaattact
    30181 ttttaccccg ccatattgat tatcaatttc ctttagcctt tccgggctaa tctcaaacac
    30241 atcttcattg ttaagttcat ccacgtaatg ggtgtacttc ccagtcattt tattcttttg
    30301 caaattattt tttagtgcct catggcgttt gattcttacg ataaccttcc catcttccgt
    30361 ttgggtaaaa agcgatttaa ttaattgctc cccatccttc ccaagattta aaatgcaatc
    30421 cagagatgac aaaagatagc aactacctcc aataccttgt cggatggtaa tagggccttg
    30481 ggttgggaac aaaggtgtca ttgtggtcct cttaaaatca taaagtacat aaagaggaca
    30541 ttataacagc aaaatattgg ataatcatta aaaaaattgg tctgtaatca atcgggaaac
    30601 caatacccta aaagctaatt ccactcatac caacgatcaa gcaaggaaat caactcttca
    30661 acaccatcct ttttcacaga agaaaacgct tgcacggaaa ccaaatgctc tgccagctca
    30721 taatactgcc gaactttaag tacggcattt ttgacttcac tacgacttaa cttatctgct
    30781 tttgtcagta aaatatgcac tgggagctct ctgtgcaaag cccaattcac caatatttga
    30841 tccaagtctt tcaatggatg gcgtacatcc atcaacaaaa ttaatcctct caaacattgc
    30901 cttgcttcaa gatagtgggc taaattcttt tgccactcca attttacttc cattgccacc
    30961 ttggcataac cataacccgg taaatcaaca aggcgccttt gctcatcaag agaaaagagg
    31021 ttgatcagtt gagtgcgacc tggtgttttg ctggttcttg ccaaattttt attattagtc
    31081 agacaattta aggcactgga ttttccagcg ttggagcggc cagcaaacgc tacctcatac
    31141 ccggaatctt cgggtaattg attgacacgg gctgcacttt ttaaaaatac tgctttagaa
    31201 tataaattga ttggcataag atatcatttt agttttggga ttttgaccca ggcagtgtac
    31261 cattaattga ctaaattatt atgagaaatc catgaaaaaa ttagcactcg tattaatcct
    31321 gtgctcttca tcaatgattt ttgcccagga aaacccgcaa gaggctggac aaagtaagtc
    31381 aacagtctgt accgcctgcc atggcccgca aggtattagt acgaatcccg aatggcccaa
    31441 tcttgccggc cagcatgaaa aatattttgt gaaacaattg aaagacataa aagaaggtaa
    31501 gtcgcgctct gcccccacta tgactgcaat agtcgctaac ctgaatgagc aggatatgga
    31561 tgacttggca gcctattatg ctaaaatgcc cgttgctgaa ggttcaaccc ctaaaaaata
    31621 tttaaaaaga ggcgaacaac tttacagagg tggtgattta aacaaacaca tcgcggcctg
    31681 tatagcctgc catggtccta gaggaagtgg aaacgctcaa gcaggctttc ccctgctttc
    31741 tggtcaacat gcagcttata ctgtaatgca actgcaagca tttaaagacg ggaagcgaac
    31801 gaatgactta aatcaaatta tgcaagatat cagtagtaaa atgagcccag aagatatgga
    31861 agccgttgct tattatattc aagggttata ttgaggaatt gactatgttt aaaaaattaa
    31921 ttgccctatt atttcttatg ccaatgactg ctttggcaac tcaatttatt gaaggcaaag
    31981 attatcaaac cgttgccagc gcgcaattgt ctaccaataa agataaaaca cccctgatta
    32041 ccgaattttt tagctatggc tgtccatggt gttataaaat tgatgcccca ctaaatgatt
    32101 gggcaaccag aatggggaaa ggagctcatc ttgagcgcat tccagttgtt tttaaaccaa
    32161 actgggattt gtatgccaaa gcctattaca cagcaaaaac cctggctatg tccgataaaa
    32221 tgaatcccat attattcaaa gcaattcaag aagataaaaa tccactggca acgaaacaat
    32281 cgatggttga tttttttgtt gctcatggcg tagacagaga aattgccaag agcgcttttg
    32341 aaaactcacc aacaatcgat atgcgcgtta acagcggcat gtctttgatg gcgcattatc
    32401 aaattaacgc agtaccagcc tttgtcgtca ataacaagta caaaactgac ttacaaatgg
    32461 cagggagcga agagcgttta tttgaaattt tgaattacct ggtaagaaaa tcagcctaat
    32521 taattcaagc tataaagaat attcctaata ggtcagtacc ctcctgtttc aatattctct
    32581 aaccgccgga tttggttgag gtgagggctg agctcttacg cacattcctc gccaaggtaa
    32641 ttggccacag gccaggtcat tttgacctgg ctttcttgct aagccccctt gcccgggttt
    32701 tccagactca aaataccggt attgaaatca taagcaaaaa tctcggcata tcgtccccag
    32761 tcaatcattg ttttcaaaac acgatctgct tctttctcgc ttaaataatc ctccagctta
    32821 ctcaaaaagc gctcttccga gacgcgatgc cccgctttct catccagtac gcgtcttata
    32881 taacgagcca gagggacttt ttccaataat ctttgcgcga acaattgctt acgctcttgc
    32941 aaatcagctt ctgaaaattg ttttcctaat tcacttaact gaatatctcc cgcagacacc
    33001 ttggcaaaac caagtatctc cagagtttca agaattggga acagatcatc aatattcatc
    33061 atgagctcgt ctgccagctc aggtaaatca atacgttctt caaaggactt catagtttct
    33121 atcagaccgg ataattcaga gggctcgaca tcgggtaagc gatagcctaa acctatttga
    33181 cgctcccttt gcgctcgctt ggctttttct ttgggtccag tagtcattaa agtataaatc
    33241 ttgtcaacca aggcaaggta ttccgggctt tccgggtcac ggggttgggg aagagtcact
    33301 ggcaattcag cacgaatata gccgggatca ttaccaaaaa tcactatcct gtcagccaaa
    33361 gtcgcagctt cttctatatt gtgtgttact aataaaatac cattggtatt cgttttcttt
    33421 tctttccata actccaataa atctgatttg aggttttctg cagtaagtac atcaagcgcg
    33481 gaaaagggtt catccattaa caacacatcc ggattaataa ccaaagcgcg agcaaaacca
    33541 acccgttgtc gcatcccacc agataactcc ttgggaaaag ccgattcgaa accatccaaa
    33601 cctataatat ctatcgcttc aatagcccta tgccttcttt cctcgcggct gattccttgt
    33661 gcttccaaac caagctccac attttccagg actgtaagcc aaggcattaa agcaaacgac
    33721 tgaaatacca tagcaatgcc ttctaccggc ctggttacag gtttaccgcg gtaagtcact
    33781 gttccactgg aaggagcgat aagaccagca ataatcctga gtaaagtgga tttccctgaa
    33841 ccagatttac ctaacaaagc aactatttcg ccttcctgta atttgaaatt aacatcttca
    33901 agtacaagaa ggtgctgaga aggtgctttt ttaaaagatt tgcttaaatt ttctatgtta
    33961 ataattgttt ccggcatagg agtctcaatt aaaattaaat cgttcttcgg ctaagcgata
    34021 aagcgggcgc caaataagat ggttgaaagc taatacatag acacacatca ttgctgttcc
    34081 taacgcaatt tgcgggaagt ctcccgctgt cgtactagcc tgaatatact cccccagacc
    34141 tgtggcctta agagtaatat ttccccagct cacgtattca gcgacgatac tggcattcca
    34201 tgcaccgcct gctgctgtaa tagctccggt aatgtaaaaa ggaaaaatac ctggcaaagc
    34261 caatcttttc caccatatcc aaccctttaa cccaaaatta tcagccgcca gataaagatc
    34321 gcggggtata ctcgaggctc ctgcgatcac attaaataaa atataccact gtgtgcccag
    34381 gatcatgagc ggagttaccc agatttccac gctcaaatta aatttgacga tagcaattac
    34441 aaataaagga taaaacaggt tcgctggaaa cgctgccaca aattgaataa tcggttgtat
    34501 tttttgagcc agccttggcc ttaatcctat ccacacgcca acgggaatcc atagcatgga
    34561 gcttagcaaa attaatataa tgacccgagc tcctgttgct gcgcccaata aaaagacatg
    34621 aaatatatca ctcaccttca attcagcgag aataaatttc aacaaaaacc acccgccagt
    34681 aataacggct atcaaaacca acgctgtcca caatcggtcc aaacgctttt gccttttaaa
    34741 atcaatttcc ttaacagcct taacctcacc aatcctcaac caacgagcat ttacaaaact
    34801 attggtaaag accgcgagcc actccgtcac tctcttcatc aaacgactac tgcgtatcaa
    34861 gtcaattaac caggattgat attctgactc atcgggagac tgttccattt taaatttttc
    34921 agaccaggca ataagtggcc tgaaaagtat ttgatcatac aaaaaaataa cgataaccat
    34981 ggtcagaatg gcatatccca cagcatgcaa atccctctgg gctatcgcca atgcgatata
    35041 agaacctaca ccgggtaatc taatattttg gtgcgccacc gaaatagctt ctgacaaaac
    35101 cacaaaaaac cagcttgccg acattgaaac catcatattc cataacagtc cagacatgga
    35161 aaaaggaact tctaccttcc aaaagcgctg ccaggctgac aacctgaaca tagcagagac
    35221 ttcaatcaaa tcatgaggaa cagtttttaa agattgataa aatccaaaag tcatattcca
    35281 cacttgggcg ctaaaaatag caaaaattga cgcgcattct ggacctaaca agctattggg
    35341 aaataaatga ataaacccgg tgacagtaat agataaaaaa ctcagaacag gaatcgattg
    35401 taaaatatca atcgccggta taacaatttg ctcagccctg cgatttttgg cagctaaagc
    35461 acctacaata aaagtaaaca gaattgaaaa aatcaatgcg ataaacatgc gtaaaactgt
    35521 tcgtaacgca taaaagggaa ggttggaggg ttccaaggaa atgggaattt gatcccctaa
    35581 ttgatacggg gtagccatct gtgaaccggc ccaacccagg aaaaataaca cagaaaaagt
    35641 caatataagc aatagcaaat cccatcgatt aataaatcga ctgaatttat caggattagc
    35701 aaaataaaac cgactattgc ccactatacc ctctcttaaa attgacgtta caagtttccc
    35761 ttaatctaat ccttaaatct aaaaaatatt tatccattcc tgaatcagga tttacgaaaa
    35821 accccaaggt caaggcaaaa aagtttttaa tgagggagtt tagataagct aaacgaccga
    35881 attaaaaact tttttaacca agaaattggg gtttttcgga agtcctgtga atataaaata
    35941 ccataaatac aacgtcatcg atacaagaca aaccaacaca ttcaaccgta tttataaata
    36001 attttgatta aattttgaac ttttttgcga ttcatgtgtc taatatagtg agcattctcc
    36061 cccgagtgct ctaccccgct ttccccctct ttatgcgggg tagtatccgg attgcaccaa
    36121 atccggatat ataattctcc ccgggatctt agccggggat ttttttgtaa tgaaaaaatg
    36181 actaaactgg cagtattgag ataatcgatg agaatttatt atgaacaaaa caatcactat
    36241 tgccttgagt ttacttctaa gtattgcctc aaaactaact tatgcagggc cagatagagc
    36301 acaattggca gtttgggcta atgaagcgat tattgccact tacacttttg attataaaaa
    36361 ttacatgcaa caacaaaagg aaatagccaa atatttttca gctgatggct ggatagccta
    36421 tagcaaagcg cttaatcaat caaaactgcc tgaagtagta caaaaaaatg cctatttcgt
    36481 caatgctgtt gccacagaac cacccaagct gatcaccctg gatccgacac actggcaagc
    36541 tatcatgccc atactggtcg tatataaaaa cccccaatac gagcaaaaac aaaatttaaa
    36601 agttatatta gggtttaccg ttgcatctcc cggacaaggt gtaagaggtt tcagcataac
    36661 cagcctgcaa tccaccccca ccagcccgcc atgccaatgt aaatttgaag agacgcctga
    36721 gaatactaaa caaggagacg ccaaacaata ataccaatat gcagcaaaat taatgagctg
    36781 attagaatta aaataaaagt tagtttttta ggtatggtaa catctaaagc agattgttca
    36841 ggacggttgg gattatagta gacgtcaatt tctgagttat ctctgtaagc tattgccact
    36901 ttatatgcaa tacttcggga atatttacta ttgggtgtat taaatgttgt atccaaaaac
    36961 aaatattccc ctgtcaaatc cttctcataa acttcataga tatattctat tctaggccaa
    37021 acactatgcc ctactcttgt ccactcacag cgcgtgacat gccccttcgc ttttaaccag
    37081 gatcttgctt caaccaaatc tcttcttttt ttccaaaaat accaaaaaag aattaaaaga
    37141 aaacacagcc atcctaaatc aaggatccat ctccaaatta tcggccacac catattcttt
    37201 cccataaatt tatattatat gctatttcca gggagaggat catgcaaaaa aaatcaaatt
    37261 ggaaacctcg caaaattaat cccaacaggt ataatcacat ccaataagtg ataaaaaaag
    37321 gaaatgcaat gactgctaaa actcctcttc acactacgca cttagcatgt ggtgccaaaa
    37381 tggtggattt ccatggttgg gatatgcctc ttcattatgg atctcagtta aacgaacatc
    37441 atgctgtcag aaacgatgct ggcatgtttg atgtctcaca tatgaccatc gttgatattt
    37501 taggcgccgg tggtcgtcaa tttctgcgca aactgctaac caacgatgtc gatcaaatta
    37561 ctcataatgg aaaagcccta tacagctgta tgtgtaatga acacggcgga attatagatg
    37621 accttattgt atatcaacgc gcttccgata attaccgagt ggtacttaat tcggcaacca
    37681 ggcaaaatga tgtggcctgg attcgcgcaa aaagtgaagg atttgctgtt ggcttgcaag
    37741 aaaggcgcga attatcaatg ctcgctgtac aaggtccaaa tgccatagcg aaaacattga
    37801 gtattcttgc acccgcccat gtagatgccg tttcaactct tacccctttc gaatgcgttg
    37861 atgtagatca ctggtttttt gctcgcaccg gctatacggg ggaagatggc cttgaaatca
    37921 ttgtccctaa tgaatttatt actcaactct ggaatgatct cctgaatgct ggagtaacgc
    37981 catgtggatt aggggccaga gataccctga gattagaggc aggaatgctt ctttacggcc
    38041 aggatatgga tgaaacaaca accccactgg aatctggttt ggcctggaca gtaaagtggg
    38101 agccggaaga tagaggattt atcggaatgg gcgctttggt ttctcaaaag caacaaggaa
    38161 ttaaacgtaa aatggtcggt ttaaccttgt tggacaaagg cattatgcgt catggccaaa
    38221 aggttattat tgaaggctgt cccgatggaa ttatcacaag tggcagttat tctcctaccc
    38281 tgcaacaatc tatagctttg gcacgagttc ccgtggaaac aggggaacaa gtacttgttg
    38341 acatacgcgg aaagttaatc ccggcaaaag tagggaagcc tcgctttatc aaacaaggaa
    38401 aacctgttta aattttagta atattaatat taaggatata caaaatgaat gatttgaaat
    38461 tcacaacaac ccatgaatgg ctgcgagaag atgaggaaga ggtcacagta ggaattacag
    38521 atcatgcgca agaattattg ggggatatgg tatttgtaga actacccgaa ataggtgatg
    38581 aagtgagtgc aggccaagaa ttaggcgtgg tggagtcagt gaaagcagct tctgattttt
    38641 atgctcccat tagtggtgtt gtaacggcgg ttaacgaagc cgttggtaaa aaccctgccc
    38701 tggtaaacca cgatccctac catgaaggtt ggctcgtgaa gctaaagccc agccatcctg
    38761 atgaaataaa aagcctgttg agtgatgagc aatatcaaaa tgagatagct gaggaaaatt
    38821 aattatgcct tatattccac acacgcccaa tgataccaaa gagatgctag aggcaattgg
    38881 tgctcaagac attcaggatt tatttgatga aataccagct tctttgcaat atgcaggatt
    38941 tcaaaatatc cctgccggca ttaacgaaat ggaaatgttg aaagaagccc agaatcaagc
    39001 ccaaaagaat cgcaacggca tatgctttat tggtgctgga tgttatgagc atcatattcc
    39061 agcagccgtg tgggatattg catctcgcgg tgaattttta actgcctaca caccctatca
    39121 agctgaggcc agccagggaa ctttgcaatt attgtatgaa tatcaaacca tgatctgtga
    39181 gttgaccggc atggaagtat ccaatgcgtc aatgtatgac ggagcaactg cattagctga
    39241 agcagtacta atggcagtcc gccttaataa acacagcaaa accaacaggg tactgattgc
    39301 aggcacagtt catccttttt atcgcgaaac cattgaaacg atagtacgta atcagcatat
    39361 tgaagtcatc accctccctt ttgatgaaca gcaaggaatc actgatcttg gctctctcaa
    39421 tcaatacacc ggagaagata ttacagcttt ggtcatagcc caaccgaatt tctttggttg
    39481 cctcgaacaa gtcgataaaa tgacttcctg ggcacatcat aacaaaacaa tcagtgtagc
    39541 ttgtgttaac ccgacttcat tggctttatt aaaaccaccc ggctcatggg gagaacatgg
    39601 ggtagacatc gtatgcggtg aaggacaacc tttaggctca cctatggcat caggaggccc
    39661 ttattttggt tttctgagca cccgtatggc ccatgtcaga caaatgcccg gaagaataat
    39721 tggccgcaca gtagacaagg atggaaaaac cggttttagt ttaactctcc aggcaagaga
    39781 gcagcatatt cggcgtgcta aagccacttc aaacatatgc accaaccaag gtttacttgt
    39841 aacagcggca actatctata tgagtctttt agggccggaa ggtctaagcc aagtggcaac
    39901 tcaatgccat caaaacactc atgaattgat tactgcctta acacaaatcg agggggtaga
    39961 actggcattc aaagccccgt ttttccatga agccttgatt aaactaaacc aaccagttca
    40021 gtacgtattg caacaactgg ctgatgctgg aattgctgga ggttatgcac cggaacaaca
    40081 ctaccctcag ctagccaata cgctgttagt ttgtgcgaca gaggtgcgca ctgcggagga
    40141 tatcgcgaaa tatgcaaaaa cattaaaaac cataatgtcc aagcgaggtg cctgatgttt
    40201 attgttcaat taacctattt agttcccatc aatgaagtaa acaaatatct gcaggcacac
    40261 agggagtttc tagattacca ctacaaacaa gggctattgg ttgcttctgg ccccatgaaa
    40321 cctccaaccg gcgggattat tatagcagcg acaaatgaca gggcccattt agagtcagtt
    40381 tttaaacaag acccttatta tttagcagaa attgctgagt atcaatttat cgagtttact
    40441 ccagtaaaac acaggaatga actcaaggaa cttattcaaa aaacggaagg caaattatgt
    40501 tgatttttga attatctaaa actggtcgcc aggcaaaagc ccaaataccc agagcagtag
    40561 gcaaaaacta ttcaataccc gaagaattcc aacgaaagtc gcccccaaga ttgccggcct
    40621 gctccgaatt gcaagtggta agacacttta cctgcctttc ccaaaagaac ttttccatag
    40681 atactaattt ttatcctttg ggctcatgta ccatgaaata caatccccgg ggtgttcata
    40741 aagcagcttc gcttcccggt tttataaatc gccatccgtt agctatggac aatgaaagcc
    40801 agggatttct ggaaactctt tataaattac aaaattatat ttctgaaatt acggggatgc
    40861 ccggtgtttc actaactcca atggcaggtt cacagggaga atttgccgga gtcgccatga
    40921 ttaaagccta tcaccagtct cgtggcgaca ccgcccgaga tgaaattctc attccagatg
    40981 cggcgcacgg aacaaaccct gcctctgcgg taatgtgtgg cttcaaagta gtagaaatag
    41041 ctaccgctcc ggacggtgac attgatcttg acgaattaaa aagaaaagta ggtcccagga
    41101 ctgctggaat tatgctaact aacccatcca ctttgggatt atttatgcgt cagataaaag
    41161 aaatagccag tcttgtacac caggccggag gattattata ctacgatggg gctaatctca
    41221 atgctatttt aggtaaagta agacctggtg atatgggttt tgatgtcatg catcttaatt
    41281 tacataaaac atttgctaca cctcacgggg gtggcggtcc tggcgctggc cctgttgctg
    41341 ttggcaaacg ccttattcct tacatgccct tacctgttgt gaaaaaaacc gactccgggt
    41401 atcactgggc aactcgtcag gattatccac aaagcatagg aagattatcg tgctttatgg
    41461 gtaatgccgg cattttatta cgtgcttatt tttatatgct tgtccttggt aaagaaggcc
    41521 tattgcgcgt atcagagttc gcaacactta atgccaatta tttacttaaa gaattgacta
    41581 aagtgggcta cacagcagcc tatcctgaca ggcgcgcatc gcatgaattt attcttactt
    41641 taaattccga aaagaaaaat tatgacgtga ctgccatgga ttttgcaaaa aggttattgg
    41701 actatggggt tcatgccccc actacttatt tccctttact ggtaccggaa tgcttgttga
    41761 ttgaaccacc tgaaacagaa agcaaagagg aattggatgc ttttgtagcc gtgatgaaaa
    41821 ccattcgtga agaagctagc aaacaacctg atatactcaa aactgcgccc cacactttac
    41881 cagtcaaaag actggatgat gtgaaagcgg ctcgcgaact ggatttaaat tattttgcaa
    41941 ctcacgagta atctatacaa cagatttttc caattttagc gcagcagctt tctttttatg
    42001 acagttgctg tgttttatga tggccaatct tctctcgcca aaaatctgat gcccttttat
    42061 aacaattcac aaacttccgc attccaagac tctcaagcat cgcattatta actttataaa
    42121 ataatgttat aatgtcattc caatggatca aataaatggt tgtagaattg gatgagccac
    42181 cagtcagaac gttgctagcg cgtcagggaa tctatgataa gaatagcgca atctttgctt
    42241 atgaactctt atatcgtaat agtgaagcac aaaattcgca ggtagataat ttaaatccgt
    42301 tgtcaggtga agcagctacc tcgtctgtgc tattgcagtt atttgccaat cttgatgtaa
    42361 ataccataat aggcaataaa caagccttca ttaatttcac ccacaaccat cttgtacaaa
    42421 aaatcccaat gctgcttcct aaaaatagaa tcgtcattga agttcttgaa acggtggcga
    42481 ttgaccaagc attactgctc aatttaatgg aattaaagaa acagggatat caaattgccc
    42541 tagatgattt tgtcttcagg aatgaattag ctcctctggt tgacatggcc gacattatta
    42601 aaatcgatgt gcttcatctt gaccagcaac aaatcgcgga acaattatta ccgctgaaat
    42661 cttttagagg taagttactg gcagaaaaaa ttgaggacaa gcaacaattc aatcattgcg
    42721 tcaatcttgg atttgatttt tttcaaggtt tttttctaaa caaaccggat tccttaacag
    42781 gacaaatcat tactgaaaat aaaatacagc ttttaagatt attaacagaa ataaacaatg
    42841 aagaggtgcc aatccaacgc attgaagaaa ttatcctgca aattcccaaa ctaagctatc
    42901 gtatcttgcg tttggcaaat tctgcttctt tgtacatgag caaaaaaata gaatcattga
    42961 tggatgccat ttctcaactg ggattaatgc aaatacgcaa ttggttaaat ctgttactct
    43021 tggcaagtct cgatgatgtc gcacctgatt tgttggagcg aacattaatt cgcgccaaaa
    43081 tgtgtgagtc cttagcaaaa tcaatggatt atcctaactc tcatcaagct tataccgtag
    43141 gcatactttc tactttggat ggtattttaa atgagcctat gccttcgcta ttagcaaaaa
    43201 ttcggttaag tgaagcactc aatgaagcgc tactgaacta taaaggggat ttaggcaaaa
    43261 tcctgaaatt tgccatagat tatgaacaag caaattttaa tcaattagaa cgcattcctg
    43321 taaaaagcga aacgctctct caatcttatc ttaagggaat agaatacgcc aatcatgtga
    43381 tagacattat taataaataa ttgtcgtccc cattattatt cattctgtat tcaacctgtt
    43441 ttacaaaaga caatgctatg gaaattgtcc atatcagata gtacaaaagg gattttcata
    43501 ccacgaagaa ctcactaatt tttatttaaa atcacaaaga tcttaaattg tccttataga
    43561 tcaaataata gcttgatgtt tcttcttgtt tatttataat tcatggaatg ttaaatttat
    43621 tctcttattt caaattatga tcatacgtga actgtttaat tggatacaca atgatagcgc
    43681 aacccttcat ctcagtgatc aaacgatcga acgtgatctt attgaacaag aggaatcaca
    43741 agccaaacaa gctcatcgtg tgcttctggg taatgcccgt cttttaaaat atagtggttc
    43801 atccaataca gaggcgatta aagaattaga gcagcattgt ctttttatgg attatctcca
    43861 gttgtataaa caagagtttg ttaaggatag aaaaaacact gtttttttaa ttgcattaaa
    43921 aaacctgtta tcccaccctc atgaagagca acttcgtctc atgcctaaaa ttaatgagtt
    43981 attccaaata ttattacgag acaacaagga aagtgctcta tctttttatg ataaaaataa
    44041 ggaattattc aggcactgta cagaaattca agaaagggta acgtttgagc tcctccagca
    44101 aaaaatggag gctgacagta aattagtaat gactcagcta gactatttca atgaacattt
    44161 agcctaccag gaaaatccct taggaatcat tgcgctatgc cgaaattgga ttggtgaaac
    44221 agaaaaattc actgctttaa tactctggtt gctagaacgc aaagtaagcg ttgaaaaaat
    44281 tcttcttact aatctgctgc aggattttct taaatatcat ttattcactt tgcattccaa
    44341 ggatagtgaa gtttctcgct tatactccct gctcggccat ttccctgaag ctcaggaact
    44401 cgttatggca gcccaaaaga taagctgtgg agaattaatg ttccagcaat actctcttga
    44461 cggcattttt cggggtaaaa atcttccagt tgtttcacca gaaatccccc ctctgcaatt
    44521 ttccttaaga aaggacaatt ttaatgcatt ataccgaact tttggccagt attttttaac
    44581 tgcaggagtt gctactgcaa caaatcatag tgacgtagct tggctggata tgttaaggca
    44641 tactctgaat cagccagaga cactcaaatt actgcctgat attatcaata ttattgccag
    44701 agaatattca ccgaaagtct taaaaaccct tgcagaactc attgcggagt ctactgccca
    44761 tcgacttctt actcttaacc aaagctgtgt ttttcattta ctacaacata aaccaagatt
    44821 acttcacgat attacagaag aaaatgttat tgagtatatc aatcttctgg ttcgactgga
    44881 tactcatgat caggaaatta tttatcagtt aatggctttg tttagggtgt tattgaagaa
    44941 aaaccatccg gcaacgaaag cagtattcga agcaattctc gataatcttg taaatcatcc
    45001 gcaactgctg gaggatgaag aatttttaac acagttaaaa aaatatccgg attgtgagct
    45061 ccttatagag gaacgttgtg aaaatatcct aaagcagctt aatttttgca ttgctgaaca
    45121 aacatcagga ctactctttg gcaaacataa ctactacatc atagaagatg tttggcttgg
    45181 agccttaaga aaatttgcag tactccagca aatcaaccca caaatgaagt ctagctttgg
    45241 gcataagtat gcccttcaag caagagttgc cgaggtagtc tttatccatc agggagactt
    45301 gtttgattta gataccttta tcgacgcgtt ggatttgccc cccgtcacct catcaggaga
    45361 aatcagtcct tacgaacggg ctcttatcga aattcttgcc acgatagata atgatctcat
    45421 cagaaaacaa atcattcaaa agctggaaac aacccctttc aatagacttg actggtatga
    45481 aagggaatat ggaaatcaaa ctgttttcat taaagcagca aaaaagggta atttaggctt
    45541 aatcaatttg cttgaagaca aaatgaaacc ttctgtgctt aacaaagcgc taagagccgc
    45601 agccaaaaac tatcaatggg aaacattgga tcatctttgt agtcttcctg gcgttgaatt
    45661 aaggcaagat gaaatggatg atcttgtggt ctatctggca gaacatgggc gaatagaaag
    45721 tgtaaaaaaa ctcttgaaat tatatgatta caaaccatcc acagaattaa ttggcacgat
    45781 tcttaaaaaa gcaatagaca atgataattt acaggtcgtc atgtatttct gtaagctgcc
    45841 agtggaatca cccaaacaag caatgcttga tcgcctattt aaattggcaa tccaattaca
    45901 gcattggggc attgttgagt atcttgccaa ctcaaagcat tattctccat cccaagcgac
    45961 gcttgaaaaa gcttttcaac aaactgcact ggctatgcaa catgaagctg tagaaattct
    46021 tggcaatgtg gaaaagactc ccatacgtgc aatagttatt gagcgggctt tactgaaagc
    46081 cagtaaactg gggcatgcaa aggttgttca atcgatttgt gccttacctt ccgagtcatt
    46141 aaccaaacaa gctattgaag acgctctgga acaagcagcg gccgaaggac atttggatat
    46201 tgtctcctgc ctctgtgagc caggaaccac aacgttaaag caaccaggga tcaattcagg
    46261 aatgaaaatt gcggtccagg caggcaaatt atctgtggtt aactatttct gctcgatgac
    46321 agggagcaat aaaccaactc cccgcttaat tgatcagaca ttagtgatgg cggctaaaaa
    46381 gggacaaaca gccattttta tcgccattca ttcaaaccac caaactcctc ctggaaaaca
    46441 tgctattgag caatcctttc aacttgctat cactgcagga aaattaccaa tcctagacta
    46501 tttgtgccgt catgaagggt atggtctcaa tcagagcaag gtagatcaag cattgatttc
    46561 agcagtcaaa tccaaacaac tggaaattgt tagctaccta tgtgaatcac tggaaattac
    46621 acccagtcga aaagcattaa gaattgctgt aagtaaagca atttccagtg accaaactgg
    46681 cttggccgaa tacttgcgaa gccgatcgac ggacaaatca aaactcacag ataccctcgt
    46741 tgatgagatt gattccacat ccaaggttgg caaacaatta gaagctaacg ggctatttaa
    46801 gaagaagaaa aggcaaaccg acagagagcc tcagatactt tttaatcctg ctatttaaaa
    46861 ctattggtca gtagaggaga atattctttc tttaatttcg cttgaccttt tgtacctcgc
    46921 gattattttt gcgcaaaata tttttatcat tagttggtat atacaaataa taaccatatg
    46981 agcagggagt cactaaaccg tgtgcactgc cgttaaattt gtctgactca ataactggtt
    47041 ataaagagaa aatcgcgtga ttggaaacca gatcaggcaa ttgctcagcc aattgcaggt
    47101 ataattttgt ttaaaaccgt ccataaattt gcgacagttt gcacgaaatc tccctcatca
    47161 tacttcgata tccagatcaa acaatgtcag agaatccacc tcaccatcgt aaattttctt
    47221 aaaccaagtt ggtatttttt tcgttgattc agaagcataa cgtgaacctt cttcatactg
    47281 tcctggaaga tagctgttaa ataatttttc taaagcgcgg ttatcatcaa ttaacagatt
    47341 tatttggtta accagataat aagtttctcg gggtttaatt ttttgtcggt gaccatattt
    47401 atctagccat ttcaatgaag ccaaaccaat aaataaagac caggcacaaa ataagcgata
    47461 cgggcgtcct tcaataattg ggatgctctt tacataagcc agtgaatgat gtgcatgaat
    47521 aaccaaactc tcgcgcaagc tattttctga agaaataaaa taacgcccac ttgtttcatc
    47581 atccacttta tctttcaaga gattgatttt ttgtaaaaag aaaccaaagt gaaaggcttg
    47641 atttaacaga ctggctgtcc acttaaaatt agatagtaaa tcagtaaata tgtgcgacaa
    47701 taatttgcca accaatcctg caacaaaaaa acagtattga ttagtggtaa ctaatgaagg
    47761 aaatacgatt ttgccttcca tgtgataatg attaaggaaa tagtgcattc catccactac
    47821 ttgcaaaatt gtttttatca cagattgctt gatggattca ggtaaattat ctttttcttc
    47881 caacaaaaga gggacggcac atagaagttg tttctcccct aatggaagct gcgaaggaaa
    47941 gctggaaagc cattttgtga attgatcgtt tgccggtgtt tgaactaaaa atgatttaaa
    48001 agcagcaaat gaatcacttt gtgcttgaag atcttgccat ttggcatctt caatactgtc
    48061 tgcaacacgc aaaagtaaat aactcaacgc aacccattct tttgctggag aagttaactg
    48121 gcttatacaa aaactaaaac ttctgcttac attttctaga tgttggtaat aaaaagaaaa
    48181 cgatcgcata accatcagcc aagttgatgt tcgcttttcg ataatgcctg ttttattctc
    48241 tcgtaatttt cttccactgt ttttgacaac tcatcatatg aaatataatt tttccataca
    48301 tgcattaaag aaaataaata cagaaaccca aactccaggt cgttttccaa taatgtatta
    48361 ctgtacttga taaagttatc taaatttgaa aagggttttg acagtggctt tatcctggat
    48421 tgctccatgt cttgaaacat ttgatcaaaa gcctcctgca tataacgcgg gaacgggata
    48481 ttgccatcaa aaactcggtg atcaacactc atgcctacag gcaaaacatc ttgcacctca
    48541 aatgtgttat ttgttttact ccagacttgt tttctttcaa tttctgtcaa ggttatttta
    48601 aacgtttcat ttggaaataa gggtgaaacc gctgcttgat atccccaatg accaatatta
    48661 ctcaaagaaa tagcgggtct ggcaaataag aaatcacggt actctctctc gtgccgtgga
    48721 gttagcaagc gattgactac ctcgattaaa tagggatgct ctttttgtaa tgattgtgtt
    48781 ttttcataac agtacttcat gatgcgcatg tcatgctgaa tgtgttgcgc taattcagtc
    48841 atagtcatct catggcagtt tttaaactca atcgcaccta attgatcact acctggaatc
    48901 tttaccgcaa gcgctacgta actattgtgc gagtggtaca gtttatgatt gaaaatataa
    48961 tttctcgtct gcggattatc aaagcaagct ttactcaatg cttgtagcat gaggcaagtt
    49021 attgtttccg gtttaaattt cttttcaaaa aaacaaacag gagtaacatt tatattaaaa
    49081 atagcagact cagttggtaa ttcggggtca ttccaaatcg ctgaaagtaa tttacgaata
    49141 ttggtcggtt caatggcttt gtctttttca aaatcatgcg ataaattctt catcaataaa
    49201 ggcatggtat ccataaacaa ttgccaatgg gctgaaggcg ttaagccaat ttttttaagc
    49261 atccaatggt gtttattatt gatgttattg agcagtttca gtgccccctc tttgcccaat
    49321 agaataccaa gggtagtcaa atattgctca tgcgcaaata cggtatttat cagctcatct
    49381 tcaaaaattg ctaatttttt ccttaaatag tctttatttg gcaacccaat ctgacgatat
    49441 aaatcgtact cgtccatatg ccggctttca tcttcaagga ctgtttcaaa aatagttact
    49501 gcgatgtttt tctctttcat tgcaatgcat aattcttcta cccatccttc tccaaccaaa
    49561 ttgaccaaag tgatagaagt ggccagatcc tcttcgcctt caagagaaga aataaaatgt
    49621 ttgatactct tttgatggcc aagtggtaat gcgtaaggag cggacaactg aaacgcaact
    49681 tttgcaaaaa cagtggcatg gaaaatctcg tcggtaattt gttgacgcat ttgttgatac
    49741 aaatctgttc cacggagttt ctctagagat tttataagcc ctcttaggcc aattgtttct
    49801 aattgagtaa acaaggaaaa taaatgaata tagataagct tgtcatgctc aagttgaaaa
    49861 ggaatttcgt tgtaaaatat cttgtccatt cggcttttga tggtttcctt ttcctcgtca
    49921 ttcaatacag accaagcatt atttaaagac tgctccgcag cccaagttgg ttttaacaaa
    49981 tcgcctaact gaatcataaa ttaaccccaa tccatttgtt atattgcaaa cgcctacact
    50041 cttccggagc acatactaat acttaagggc aagtgtacta ttaaagcgtt tttgtggata
    50101 ttgtcatata ctgattgtaa ttgctactgg atcccagcct gaaaataacc atagataaaa
    50161 aatagataaa aaatgcgatg cccctcagat aaaaaagact tcttaaaatt atcttatcga
    50221 tgagcgataa accccctttg cttaatgagt tattcgcaaa tctcgagagt ctgagatgac
    50281 tggttttcgg cgcaccccag cttctcaaca ggatcgacaa aatacgacct gatatctcat
    50341 gcaaaccaaa gtacattagc tgattgacga gtcatttaaa aatcaaatct ctatagtaaa
    50401 ataagcatcc cggaactgtt tgcaatgcag caatataaca aaagggacag attagagaga
    50461 ggggctatta tctcaataga aaataattga ttttaatagc aaaaatatct ttgctattaa
    50521 aaaaaaataa gttataacac tcttaactac atcaaggatt gattagtgca aatgttgggt
    50581 caacatctca gtaaattcat gttgagttga ctgtataatt ttcatggagt gaaaaatgaa
    50641 tagtaaagta ttttcgcaac gttttaatcg tgaattggca actctgggat tccccgagga
    50701 tttaacagac aaaacgaaag ccgtatcaaa agtatttggt gtcacacgcc atttggctaa
    50761 tgcaatgata tttggccacg ttcttcctga taaagaacaa ctggataaaa tcgcagaaat
    50821 tctggaggtt tgtcctcaat ggctaagtgg ggcaacggat aggaaaaaag cctactcagg
    50881 ccgtgagacg gctgatagtg tgtaataaac ctggaatttc atgtatactg taagctaata
    50941 gacagcgttt gctaaaatta tttactttgc aaacgctttt ttcattgttg ggttattaaa
    51001 atcatggaat gcttaagttt ctgtgtggcc aaaaccatag acttgactcg tcttgatctt
    51061 catctaaaaa acgtctctaa ggaatttagt gctgtaaaaa cccgagatgt aataagatta
    51121 aattctcata gaaacaaaga tcacaccctg ttcattttta aaaatggaac tgtcgtatct
    51181 tggggtgtaa aaagatacca aatccacgaa tacctggata ttattaaact gcttgtagac
    51241 aaacctgtag cacttttggt gcatgatgaa tttcattatc aaattggtga taaaacagcc
    51301 atagaaccgc atggttttta tgatgtggat tgcctgacca ttgaagaaga cagtgatgaa
    51361 ctaaagctca gtttgtctta cggcttttct caatctgtaa aattacagta tttcgaaacc
    51421 attattgacg cattgattga aaaatataat ccgctgattc aggccttatc tcataaagga
    51481 gaaatgccta taagcagaaa acaaattcaa caagttattg gtgaaatttt aggtgccaaa
    51541 agcgagttga atttgatcag taatttctta taccacccca aatatttttg gcaacacccc
    51601 actctagaag agcatttttc catgcttgag cgctatttac atatacaaag acgcgtcaat
    51661 gccattaacc atcgtcttga tacgcttaat gaaattttcg acatgttcaa tggttatttg
    51721 gaaagtcgcc atggccatca cttggaaata attattattg tattaatcgc agtagaaatt
    51781 ataattgcgg ttatgaattt ccatttttag ggtttttccc ttaatttatg cccactctaa
    51841 aatcctattt tctcaaagac acctctttgc gcacagaaat acttgccgga gcaacgacct
    51901 tccttaccat ggtttatatc gtttttgtca atccaaaagt cctgcacgat gcaggaatgg
    51961 atcaaggcgc ggtatttacc gccacttgtt tgataactgc tttcgcctgt gcattaacag
    52021 gcttcatagc caacacaccg ataggtgtag cgccaagtgt ggctttaaat atttatttct
    52081 cttacagcgt agtacaaggg atgggagttg actggcaaca cgccttggcc atggtattca
    52141 tttccggcct gcttttcctg tttcttaccc tcaccaaatt aagacgcctc ctgattgaag
    52201 caatccctca taatttacaa atagccattg ttataggtat cagtttgcta atcgccttaa
    52261 ttgctttaga aaataatcaa atcattattg gtgaccagca caatttaatg cggttagggc
    52321 ctatcatcag accagaaaca ggactctttt tcctggggtt tattcttatt cttactctgg
    52381 attatcataa gataccaggt gccatcatca ttgggatact gagcatcagt atcttaagct
    52441 tgatgttggg cttgaccagt tggcaaggtt tgattgctat gccaccgtcg ctcgaaccta
    52501 cgtttttgca gcttgatttt tctggtcttt ccagtatcgt ggctttaaaa accattttta
    52561 ccttttttct gatcaccgtg tttgatgcga caggatcatt aattggctta ctaagtccaa
    52621 ccatttttaa acatgaaaaa aattattata aacgcctaac tcgcagttta actgccgatg
    52681 cagccgcatc ttccctcgca ggcctaatgg gctctgccag tacctcacct tatattgagt
    52741 cagcaaccgg aattgaagcc ggtgggagaa ccgggctaac cgcgttagtc attgcagctg
    52801 gatttattct gatgctgttt ctttttccac ttgccaaaat gattccaagc tttgcggttg
    52861 gaccggcttt actctacgta gcgtgttgca tgataaaaca tattactaat atgaaattac
    52921 aggatatcac cgaaacagcg ccttgcatgg tcatcatcat gatggtgcca ttcaccgctt
    52981 cgattgccga tggaataggt ttgggaatta tactttatac ccttcttaaa ttatttaccc
    53041 gtcaaaaacc caatcctttg attattgttt taagccttgt ttttatcgtt ttctttctga
    53101 ttaactaagt gaatgtgatc tttaaatcct ggataaaccg aaaagcttcc ataagaaact
    53161 tgtctactct caaaaactgc aaacaaaagt ttctttaatt tattgaattt agtagtattt
    53221 aaataagtga attatgcaca agatagggcg ttatcttaaa tcgttcgttt aatatttcgt
    53281 gccaaagctt agagacgatg cttaggcatc tcctcagccc aaacgggcat cggatatcga
    53341 atgcttctta tataatactt gtccataact cacgatagat agccaaataa ctgccataag
    53401 ctcatagctg gcctaatcgc gatgaattat tatgcttgaa caatcattac gacattatta
    53461 tcagcttgtc ttggtagatc ccatactacc tgctttacgt gacaggataa cgccattgtc
    53521 cattacctgg ttgtcgggaa tatttggctt gttgtttatt ccttttgtct tattagacaa
    53581 accgtatatt gcaattagct ttctcttgtt ttcgggatac ctggataccc ttgatggttc
    53641 gctggcccgc tttcaaaaca gagcctctga ctttggctca gcaatggata tcgtcatgga
    53701 tagaattgta gaattttctg ctgttcttgc tttgtatctt tttgatccac aacaccgtgc
    53761 cactttagcc attttaatgt taggcagtat tttgctctgt attacaagct ttctggttgt
    53821 gggaattttt tccaacaata ctactcaaaa aagtttttat tacagcccgg ggataatgga
    53881 gcgtgccgaa gcatttctat tctttatttt tatgacatta ttttccaact gctttaattt
    53941 tcttgccgct ctcttttgtt ttttagtctg tttaaccgcg ttgataaggc tttttgaatt
    54001 taaaaaaagt gtacgggctc caggttagcg aaacaataag ttttccgaat cctgatttaa
    54061 aactttctct gattttatgc tgactccaat acaaaatctt gtataattta cagcaattca
    54121 taaaagaggc tacaatgttg cgtatttttt tgtattttct cttactcatt aacacaacat
    54181 tttgtctggc aaagactgaa aaagccattt ttgcaggtgg atgtttttgg tgcatggaag
    54241 cagactttga ccatcttcct ggagtcctgt ccaccacatc aggatttgat ggaggtactc
    54301 ttaaagatcc aacttatgaa caagtttctg ccggtaatac ccattacgcc gaatcggtaa
    54361 tggtggagtt tgatcccaat aaattaagtt acaaaaaact ggttgagtac ttctttaggc
    54421 atattgatcc taccgtcaaa aatgcgcaat tttgtgatca tggcagtcaa tatcgcagtg
    54481 caatttttta tcttaacgat caacaaaaga aaattgctat ggaatacaaa aaagagttag
    54541 agcaaaaatt ccccacagtt tatacagaaa ttgccccatc aacttctttt tatccggcag
    54601 aagaatatca tcaaaattat tacaaaaaaa atcctatacg ttataagtat tatcgatatc
    54661 gttgtggccg agatgcccga attcaagagg tttggggtaa taaaaaataa aagaataacc
    54721 cttaatcatg atccatagtt
//
LOCUS       DATNSN010000003        54505 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.003, whole genome shotgun sequence.
ACCESSION   DATNSN010000003 DATNSN010000000
VERSION     DATNSN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 54505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54505
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.003"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..727)
                     /locus_tag="VAH76_000126"
     CDS             complement(<1..727)
                     /locus_tag="VAH76_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO1384789.1"
                     /translation="MIIDWPVIIVLFCLSIPGVLIVMHRLVYFLLADNTERLKKRFSR
                     FVVLQTLFMTFVMSFAGSILSSRTGLGDPLLEGLLQGKASLDSFQVILLPTFLYALLG
                     LVIFCILYYGLFGSILDEKSFKIMSELRSKIGLDGCVLYGGVVEEVIARWGLMNVVCF
                     FALLFTSQIDSEIIIISIFLSAFIFAVGQLPAYIAAGCTPSRRFLYSFVLLSLSQSIL
                     FGFLFWQYGLTSAILAHMLFHLGW"
     gene            complement(724..2007)
                     /gene="rsmB"
                     /locus_tag="VAH76_000127"
     CDS             complement(724..2007)
                     /gene="rsmB"
                     /locus_tag="VAH76_000127"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO1384790.1"
                     /translation="MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFC
                     RHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKS
                     WAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNNWQFIAEAND
                     RHPPMTLRVNVLKNSVDEYLDLLKKSGIEAFKHPVVPDGITLAIPCDVMTLPGFAQGS
                     VSVQDAAPQLAAYLLSLKPGQRVLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQ
                     RVRDNLNRLDLQATVVQGDVLEPRSWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRT
                     DEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIARFVAEHSDCQVSD
                     GKWSWGRWTGHGQQILPGEHGMDGFFYSVLRKDEK"
     gene            complement(2004..2948)
                     /gene="fmt"
                     /locus_tag="VAH76_000128"
     CDS             complement(2004..2948)
                     /gene="fmt"
                     /locus_tag="VAH76_000128"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO1384791.1"
                     /translation="MSGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGR
                     KLQESPVKEWAINNQVPVYQPLNFKNQEAIDELSALKPDVMVVIAYGLILPKAVLEIP
                     RLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTS
                     SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQ
                     SAVEIDRKIRAFNPWPVAYTLAGELMLRIHQAKATDIMSTEMPGMVLNVDKNGMLVAT
                     NDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ"
     gene            complement(2945..3457)
                     /gene="def"
                     /locus_tag="VAH76_000129"
     CDS             complement(2945..3457)
                     /gene="def"
                     /locus_tag="VAH76_000129"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO1384792.1"
                     /translation="MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARG
                     VGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVV
                     RAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
                     KFKRLQARKP"
     gene            3551..4588
                     /locus_tag="VAH76_000130"
     CDS             3551..4588
                     /locus_tag="VAH76_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO1384793.1"
                     /translation="MKYCLLIFCFIISSITHALSLRPDSPSRYVVQPGDTLWSISSRF
                     LNNPWEWKALWRANPQIQNPDRLYPGAVLALEYYQNIPYLRVLSNGTIKLSPNIRVTP
                     QEDAVPPIPLGDIKPFLDESLILDVNVLNRAPYVVALMSERMLGGQGDEIYVRGLHPS
                     KEMPQGGTIGYSIFRGGRNYFDPITHELLGYKAVLVGYGELVAGGEPATVLLTSINQG
                     IKINDKVLINNHPEFELYFEPETPARQVRGYIIEMPDNMPVGNTQEAVGGVIIINLGE
                     TSGIKPGDVLAIYGKERIVNDPQNHLRPITLPQERLGEAMVFRVFTKASYALIVRSTR
                     AIHLLDTVTNP"
     gene            4585..5670
                     /gene="dprA"
                     /locus_tag="VAH76_000131"
     CDS             4585..5670
                     /gene="dprA"
                     /locus_tag="VAH76_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO1384794.1"
                     /translation="MNNLQPLLALNRMKKIGPRTVLKLQKRWPDLNLMFQLSSVDLEE
                     AGLPSWLAQTIKNFDLDFIKTDLDWLSSSENHHILTWDSPYYPALLKEIADPPFILYA
                     KGELSALTQPNLAIVGSRNASVTGNENARAFSREISRHGVSIVSGLALGIDAHAHLGC
                     LEGGGKTIAVLGTGIDCIYPRRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRII
                     SGLSLCILVIEAAIKSGSLITARMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLV
                     TSVADVLDELKIEHHQFASNKPIFSLASGKENLVKFIGFETTTIDQIIGRSGYSMEQV
                     TSGLAELELKGAVMAVPGGYIRCEYER"
     gene            5660..6097
                     /locus_tag="VAH76_000132"
     CDS             5660..6097
                     /locus_tag="VAH76_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEO1384795.1"
                     /translation="MKDNLFEMLLNLFEKSLTQLQKSHKSNDQDSQSQLLEEDLLSAE
                     EQSLFIKSAQQKSTRIFTYDEQMKLTKASYQFLMRMKLWNILDIDQFESIINQLQFSE
                     SRIVTLQETKWTIRNALASSLNEEQLTFLDLVLYQTEDELTIH"
     gene            6177..8456
                     /gene="topA"
                     /locus_tag="VAH76_000133"
     CDS             6177..8456
                     /gene="topA"
                     /locus_tag="VAH76_000133"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO1384796.1"
                     /translation="MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSV
                     NPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERN
                     LLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEE
                     KLQQFSVTQQEQAHEIKKQLIAQAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARK
                     LGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGD
                     NCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQ
                     MADAILDTVSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFN
                     VGEKIKVSDIETNQHFTEPPPRYSEATLVKALEEYDIGRPSTYASIIHTLQQREYVVV
                     EKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLEEFWQP
                     FVQQIQNIDEQVQRKDVTTELLEEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDI
                     SNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGV
                     TCPKCNQAKILQRKSRKGKIFYSCGNYPKCDYALWNEPVDLPCPKCAWPILTLKESKK
                     FGRQILCPREGCDYSAKED"
     gene            complement(8667..10640)
                     /locus_tag="VAH76_000134"
     CDS             complement(8667..10640)
                     /locus_tag="VAH76_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1384797.1"
                     /translation="MNYRSDIDGLRAIAILFVLFFHGGLTLFPSGFIGVDVFFVISGF
                     LITSIIEKSLQNNRFSFMDFYSRRLWRLQPVFICLIVVTTLVTLFYYLPDDLMLFSKS
                     ARKTSIFTSNVFFKNVTSDYFSPDSNQLPLLHMWSLSVEWQCYFILPVGIYLLYRLVG
                     PQHRIKITYLLTITFFALSMYYSWSNPAKTYYQFSSRIFEFLIGSCVASASGSKRFLA
                     NKYVLNFITVLAVLTLFYIAMHHDINIGFPNWYTLILCLATAILIAAGQQESQSIVTY
                     ALSLRPLVFIGLISYSLYIWHWPVLALMRYLNIEESNLVLLVTFGLILIIAYLSWRFI
                     EKPARKLHRIRFSYTVVCLLIMPVFVTHLSDYLIKKNAGYPHRFNEAIQVYAELNKYK
                     NPQRPLCLERKNTEVNPQCVLGAKNSDSRTGFMIGDSYSNQHWGFMDTLAKEANVSIL
                     AHATVACLALPDIYQFDWNVKNKIYQECHDQTNRYFNMIKTNHYDYVILGQNWNGYFG
                     DRIINQLNDNRSQELSLKRIEKALDKALELITTSGAKPVLIKSIALSKGNPYNCFFEH
                     IKLHKKYNPERCDFNLDVSEQIWFDNLFAKMQRKYKTLVIIDPRVAQCPSGMCKVDIN
                     GIPVFRDSGHITDYASYHLAKLYLQKNNNPLVS"
     gene            complement(10776..11216)
                     /locus_tag="VAH76_000135"
     CDS             complement(10776..11216)
                     /locus_tag="VAH76_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO1384798.1"
                     /translation="MAENTTESNVIYFTRLHWIIFFGPFVGLGLVMAIYIYIIQLREV
                     SLFLGFFALIWILITWVTYHFSSITIKRKQVILRTGIIVRQTVDIPLNKIETIDIRQS
                     ILGSILSYGTLIITGTGGTRYLINYLYKPLTCRRYIEQLMNEPH"
     gene            11347..11769
                     /locus_tag="VAH76_000136"
     CDS             11347..11769
                     /locus_tag="VAH76_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEO1384799.1"
                     /translation="MKSISQCLNKQLSTICQHSMQLEELSLKISAFLPSHFISKCQVG
                     SFNKGCLVLTTTNAAFASEIRYLLPELRDKLRKEAGLYQLSSIKITIIAPSSQIENPA
                     QKKSLTLSEKAKATIISESQHCTYEPLKKALLHLAQSE"
     gene            11787..13091
                     /gene="lem28"
                     /locus_tag="VAH76_000137"
     CDS             11787..13091
                     /gene="lem28"
                     /locus_tag="VAH76_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem28"
                     /protein_id="HEO1384800.1"
                     /translation="MCSEFFFTIMLKYSTNMLELAMPKINKIVNGTDLTPHYLSEPNK
                     EFKIYRYNKEVYAVRFENDEPMDYVLMWKSHKSGHTQNSEMIKNTEEDYKELGKGEQG
                     TVYEKTEDKAMKVSRGRHPREFYEEINLHIIEQQFFLKYHGIQEHFVLGLWNIKNEEN
                     VYFYMPKINAIPINKKIDQPKIEEFVLALKELNDAGYWHPDLANNPYHISPQNLIATE
                     EMVKTIDLDGGFRYDKGRVDELSRKSLVYGKDQWLYVYNFIYPPTDEEDNRIDWRVPI
                     EKWYENNRDESLSVNPHALLRFYHEGLISLPKKLAHDLHETILEELSQDKKFKKHSLE
                     VGVNRFFSPKKQGIYEGLKGDGLKKVILKELRDSLAEIRTMEQLEEKKLEFLASPEMQ
                     ILAEGQDKTTKALNLKTSSRKKVMVIFKEAEERILNSPNVSI"
     gene            13392..14189
                     /locus_tag="VAH76_000138"
     CDS             13392..14189
                     /locus_tag="VAH76_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1384801.1"
                     /translation="MLKQIVLTGLVIIGIVITLMYLFQRHLIYFPNRHTPKLEDYNAS
                     DMKVVSLRTKDNLHLKSWYKPASKHRPTILYLHGNAGHIGYRMPLVREFIDAGLGVFL
                     LEYRGYGGNPGKPSEKGLYADGETAIEFLIQHGVPSKRVILYGESIGTGVATHLATKY
                     PVCAVMLQSPFTSLTRLAQYHYPLNFLKPWDQYNSLARMKKINAPILVLHGKLDQIVP
                     YQEGLNVFNEANEPKKMISFDDKEHNDLWSADNFSREIIQFALNQCS"
     gene            complement(14245..14661)
                     /locus_tag="VAH76_000139"
     CDS             complement(14245..14661)
                     /locus_tag="VAH76_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384802.1"
                     /translation="MSFSLSELLHYKNEKIVTHFCYSHPEYSIQEGLFLFEDLLAWMW
                     LNKQRRLRGKKTYLFGPLLILDEMWHSFILHTRDYIDFSMKYFGEYFHHDVEPVGFEH
                     VLDEEELADYLQDCFQYLNKEWVERRFSMAFAEMDD"
     gene            complement(14776..15627)
                     /locus_tag="VAH76_000140"
     CDS             complement(14776..15627)
                     /locus_tag="VAH76_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEO1384803.1"
                     /translation="MCRFVAYLGRPTLLENVLVRPKNSIIMQSLHARETKYRTNGDGF
                     GLGWYSPEVSSDPALFTSVYPAWNDRNLLHLTAKIQSPCFFAHVRSASAGGVTNYNCH
                     PFIYGDWMLMHNGEINDFIDVKRHIRHLLDDDIYHWVKGQTDSEHLFGLFLQLAKGRD
                     INQLSVAADVLQETINKILEVIGQFGHSGPSYFNICLTNGKKIIATRYCSHEKKKPLT
                     LHYLIGYVLSTQGLWIKEEGNPAYVVVSSERLNDLDEGWEDVPPQHMILIDENKNIQL
                     RPLQTLG"
     gene            complement(15780..17816)
                     /locus_tag="VAH76_000141"
     CDS             complement(15780..17816)
                     /locus_tag="VAH76_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO1384804.1"
                     /translation="MKFKIATCIVTMLSSFIVFSSNSNFSDNNTNDLLHLDWRDTSVS
                     PAVDFYTYANGNWKKNNPIPADYASWGSFYVVNEKVQNIIHQMLINASKNTHAKPGSI
                     EQKVGDFYFSGMDVDSINKSGITPLKPEFTKIEEIKNLKDLQAEIAHLHKIGVGPLFN
                     FGSMQDFKDSTSMIGAVMQGGLGLPDRDYYLKKEAKFKQVREAYVNHLTKMFELLGDS
                     PEMAAKEAKIVMDIETQLAKASMSQVEQRDPHAIYHMMTITELDKLTPNFSWSQYLSA
                     RGQGKIKKINFAMPDFFKAMNELLQSVSLDEWKIYLRWHLIDAFAPYLSKPFVDQNFK
                     MVSALTGTQKLLPRWKRVVNTENGALGFAIGELYVEKYFSPESKQKVLDILKNIRAVL
                     QEDIKTLSWMSPKTRDAALKKLDLMEERVGYPTKWWDYSSLKIDRGPYVLNVIRANEF
                     LINRDLDKIGKPVDRSEWAMTPQTINAYYDPSMNNLNIPAGILQSPFFDPKAPTAINY
                     GAIGYVIGHEMTHGFDDQGAQFDGYGNLKNWWAPNDLSKFKNATQCIADQFSKYVVDG
                     DLHVQGQLVVGEATADLGGMILAYKAFHRSKEYKNAKTIEGITPDQQFFLGTAHVWAM
                     NVRPEQLRNQVTTDPHPPAVYRVNGSLANIPQFQEAFNIPNGSPMISKNRCIIW"
     gene            complement(17960..18874)
                     /locus_tag="VAH76_000142"
     CDS             complement(17960..18874)
                     /locus_tag="VAH76_000142"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEO1384805.1"
                     /translation="MIKQRTPKKVIQATGVGLHSGEKVLLTLRPAPVNTGIVFRRVDL
                     SPVVEIPASYEYVGDTMLCTTLHHGKVKIATVEHLLSALAGLGIDNAYIDVNAPEIPI
                     MDGSAAPFVFLIQSAGIREQNAAKRYIRILKPIRVEENGKYVQFLPHKGYKITFTIGF
                     EHPVFNDRPQTVSFDFSGTSYVKEVCRARTFGFLSDYEKLRECDLAKGGSLDNAIVVD
                     DYRVLNEDGLRFESEFVTHKVLDAIGDLYLLGSSLIGAFEGYKSGHELNNRLLRELMV
                     RQDAWEYTYFDTENYLPAVHPEYYPVEA"
     gene            complement(19122..20318)
                     /gene="ftsZ"
                     /locus_tag="VAH76_000143"
     CDS             complement(19122..20318)
                     /gene="ftsZ"
                     /locus_tag="VAH76_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO1384806.1"
                     /translation="MFELMESSQQENNAVIKVVGVGGGGGNAVEHMVAENIDGVEFIC
                     ANTDAQALRGSSAKIHIQLGDALTKGLGAGANPQIGREAAEEDREHIKEILSGADMVF
                     ITAGMGGGTGTGAAPVFAEIAKELGILTVAVVTKPFSFEGKQRALAAEEGIRRLAEHV
                     DSLITIPNNKLLSVLGKNISLLNAFKAANNVLLGAVKGISDLITRPGLINVDFADVRT
                     VMSEMGMAMMGTGSAVGEQRARQAAEAAIASPLLEDVNFSGARGILVNITAGLDMSIG
                     EFEEVGDVVKEFISDDATVVVGTVIDPEMTDEMRVTVIVTGLGDNRQRQQQPQQPLRA
                     RLVETTRSDGSLDYQQLERPAVIRKQAQTGTATTATKQTNNGNESVPDVDYLDIPAFL
                     RRQEEA"
     gene            complement(20513..21751)
                     /gene="ftsA"
                     /locus_tag="VAH76_000144"
     CDS             complement(20513..21751)
                     /gene="ftsA"
                     /locus_tag="VAH76_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO1384807.1"
                     /translation="MAKKIEKNIITGLDIGTSKIIALIGEVTSDGTIEIIGIGRHPSR
                     GLKRGVVVDIETTVNSIQRAVQEAELMAGCEVRTVYAGIAGSHIRSLNSHGIVAIRDQ
                     EVSQADVERVIDAAKAVAIPADQKILHILPQEFIIDHQGSIREPIGMAGVRLESRVHI
                     VTGSVSAAQNIVKCVRRCGLDVIDIILEQLASSHAVLTEDEKELGVCLIDIGGGTTDI
                     AIFCEGAIQHTSVIPIAGDQVTNDIAMALRTPTKAAEAIKVNHACALPELANPNQMLE
                     VTSVNDRPGRKISAKALADVVSARYEELFSLVRNELRRSGFEDRLAAGIVLTGGASNV
                     RGAIELAELCFEMPVRKGCAHYVSGLTEATENPSFATGVGLLLHGFQQQYESGYNVPA
                     MNDNGKSIWSRMKEWFQGNF"
     gene            complement(21751..22470)
                     /locus_tag="VAH76_000145"
     CDS             complement(21751..22470)
                     /locus_tag="VAH76_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEO1384808.1"
                     /translation="MEVNSVGLRYLTIMGLLILSALLLAGRLGYLYLADAERFPITTI
                     KVAATYEHITHKELENVLAKYLDASFFLLSVKGLQSELNSMSWIDTAYVERVWPDTLK
                     IKLTEKKPVAIWGDALMTRDGKLFNQGSVPSDLDIPKLKGPQSQQLEVLQVYEKLSKI
                     LSSYGLNASGLYLRDNQSWVLLLNHSVKIYLGKKELEERLLRFCKAYPAVFAEKADQL
                     AGVDLRYPRGMAVQWKQQMGR"
     gene            complement(22486..23580)
                     /locus_tag="VAH76_000146"
     CDS             complement(22486..23580)
                     /locus_tag="VAH76_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO1384809.1"
                     /translation="MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAM
                     DKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYED
                     GCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
                     QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVG
                     REIELAVLENSIPCGKPRVSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSL
                     EEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQAT
                     GVAYPDLLDELINLAMVHHNCRQHLVTNYL"
     gene            complement(23564..24490)
                     /gene="murB"
                     /locus_tag="VAH76_000147"
     CDS             complement(23564..24490)
                     /gene="murB"
                     /locus_tag="VAH76_000147"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO1384810.1"
                     /translation="MSITGMDSSHVTESQGALLFNEPLAEYTTWRVGGPAARLYKPAN
                     IDDLALFLSRLPFDEPLLWLGLGSNSLIRDGGFSGTVILTQGCLKEMTLLSDNCIRVE
                     AGVSCASMARFSARNNLSGGEFWAGIPGTMGGALRMNAGCHGGETWQSVIEVQTINRR
                     GEIRTRKPEEFEVAYRHVAGLGDEWFISAKLQLSPGNKETSLQLIKDLLAHRAKTQPT
                     NEYNCGSVFRNPPGDFAARLIESCGLKGVSIGGAVVSEKHANFIINHQGTATAANIEA
                     LIHLVQTKVREQTSIELIREVHIIGDANVQTR"
     gene            complement(24499..25908)
                     /locus_tag="VAH76_000148"
     CDS             complement(24499..25908)
                     /locus_tag="VAH76_000148"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO1384811.1"
                     /translation="MNNSEQFLSPRMGRVEQIHFVGIGGAGMCGIAEVLHNQGYRITG
                     SDLGESGTVQRLRSLGIQVYIGHRLENIKGADVVVRSSAVDFNNPEIVAARELMIPVI
                     PRAAMLAELMRFRHGIAIAGTHGKTTTTSLVSSLLAEGGLDPSFVIGGKLNSCGANAQ
                     LGKSAYFVVEADESDASFLFLKPMMAVVTNIDADHMDTYEGDFEKLRTTFLEFLHHLP
                     FYGLAVVCLEDEEICRILPAIQRPTLTYGFKEEAHYRAINWTQKGMLSEFVVVRPAPH
                     KQLTIQFQYPGRHNVLNALASIAIATELGVDDDSIVRGLQKFQGVGRRFQMLGEKQFE
                     KGAAIIVDDYGHHPQEILSTIDAFRRVWPERRLVHVFQPHRYTRTQSLHRQFVDVLSL
                     SDELLLMDIYAAGETAIPGVTSENLANEIRSRDKRVTIVSEQSLKATLDEFIKDGDVI
                     LMQGAGSIGQMAVNLMKNM"
     gene            complement(25918..27093)
                     /gene="ftsW"
                     /locus_tag="VAH76_000149"
     CDS             complement(25918..27093)
                     /gene="ftsW"
                     /locus_tag="VAH76_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEO1384812.1"
                     /translation="MRPRHLNQRGKPVSRPISLYDKWLIGAVFGLLIIGLMMVASSSV
                     MISTKYFHQPFHFLIRQACYLFVGLLLALIVVRTDSSFWEKISMPMMIGCVFLLLIVL
                     IPGIGKSVNGSRRWLALGPIGVQVSELTKLAMIFYLSGYLVRQQEAVCESIFGFIKPM
                     AILAVVSVLLLLEPDFGATVVISGTVMAMLFLAGVKLRYYFGLMLVVVTALALLAVSS
                     PYRVARLTAFLDPWADQYNSGYQLTQSLIAFGRGGWFGTGLGESIQKLLYLPEAHTDF
                     LFAVIAEELGLFGILVVITLYSILVIRGLNIGYTAYTQERHFASYTAYGLTIWLALQA
                     SINMGVNAGLLPTKGLTLPLLSYGGASMVINCIVIALLLRIDHENRWQSLGLKPLTA"
     gene            complement(27099..28442)
                     /locus_tag="VAH76_000150"
     CDS             complement(27099..28442)
                     /locus_tag="VAH76_000150"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO1384813.1"
                     /translation="MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEF
                     QNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTPVLERARQAGASIYGDIECLARE
                     ISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
                     LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYSGAKAVLFNREDVY
                     TVPHQSCQADIKCISFGKDAPSMGNWGLIEQENTTYLAKGMERLLPVESILIKGVHNW
                     MNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVGWINDSKGTNIGATI
                     SAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKV
                     VPVVRASSLEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL"
     gene            complement(28456..29541)
                     /locus_tag="VAH76_000151"
     CDS             complement(28456..29541)
                     /locus_tag="VAH76_000151"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO1384814.1"
                     /translation="MLYWLTQLLQGQYHAFRVFQYLTFRSILASLTALIVGLLCGPLM
                     IRWLRGLQIGQMVRSDGPQTHLSKAGTPTMGGVLILLAITVSCLLWCDLRQTSLWLVL
                     LVTLANGLVGWVDDYRKLVLKNSKGLPGRWKYFWQSVIALVAVSYLYWNASLPVHTQL
                     TVPFFKTVTWDLGVFFPVLAYFVIVGSSNAVNLTDGLDGLAIMPIVMVAGALGVFAYA
                     SSNAVYSNYLGIPYVPNTGELTIFCSSIVGAGLGFLWYNSYPAQVFMGDVGSLALGGA
                     LGIVAIVVRQELVLLIMGGLFVIETLSVILQVGYFKYSGGKRLFRMAPLHHHFELKGW
                     SEPKVIVRFWIITVVFVLCGLATLKLR"
     gene            complement(29656..30993)
                     /locus_tag="VAH76_000152"
     CDS             complement(29656..30993)
                     /locus_tag="VAH76_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO1384815.1"
                     /translation="MNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
                     DGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIAL
                     TGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPG
                     EIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAY
                     AHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLH
                     NVRNALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRS
                     VLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELA
                     SQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG"
     gene            complement(31234..32013)
                     /locus_tag="VAH76_000153"
     CDS             complement(31234..32013)
                     /locus_tag="VAH76_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEO1384816.1"
                     /translation="MQANWSLILNVLLLIGVLVAIGRLMKTRRQSLNQEHYQPSLGQV
                     ENNSYGSQSYNDEIIAVRKVNREVPLDFENFDGSEKRQAIRKPSVKTEQPQLIPEEEE
                     CESNERTEPNSDCSSSTAMVFLLAKENRQFAGYELLQTVLAAGLRFGERHLFHRHQFT
                     NGQGPVLCSLAAATATGVFDLQNIGAFSVRGLCLFMQISNNPEINAERFSVMLDTAKQ
                     LSEGLDAYLLDDQRKPLTEERIARYHRYLKIEHLMDDCVTN"
     gene            complement(32018..35512)
                     /gene="smc"
                     /locus_tag="VAH76_000154"
     CDS             complement(32018..35512)
                     /gene="smc"
                     /locus_tag="VAH76_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO1384817.1"
                     /translation="MHLKQLKLAGFKSFVDPTTVHFPSRLVAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDIIFNGSSNRKPVGQASVELVFDNSLGRLTGPFASYGEIA
                     VKRVVTRDGESFYYLNGARCRRKDITDIFLGTGAGARSYSIIGQGTVSRLIEAKPEEL
                     RAYLEEAAGVSKYKERRRETLQRIEQTRDNLTRVADIREELGKHLQRLERQAKAAERY
                     TILKEEEKICRAEIQALKWQEYSEKQTVKQRELQELAIRNEQQQSLLTSCYKERAVLN
                     EQLQDAIDQSQSIQDSLYQSGTEIARLEEAIQQQEREKKRLENDRQQMQVDSQLIEKQ
                     LKLDNEELHNCKLKAQQLEEHLHHLKMEFKEQESLWQEKQKEQSQWEKIWQEVQINSN
                     TIKNEYQLTQAKLQHLEQKRHQVLVRLEKLNLEQSAISLDDLQKNKIKLQEKHFQLKE
                     TQEFDEEQLRQANETQSDLQNQLQNTEQQLHALQDEFHRLNSELAALKAAQIAAKKEN
                     RSNKDGLAEWLDKPRLMDVLKVEPQWQSSCEIVLGDDLIAYVLDDFAELWSRWQFCED
                     HKESVVTMRALNGEKQERPRLADKIQGLVPSTVNHLEHIYAADNLEEAKAWFPDLLAH
                     ESIVTVNGIWLGKGWIKLPRQKEQDEVGVLARQQRIVELNLSVESLQQKIESIRLARD
                     SYQARLQENLNFIKLHQLNLNASNEALRNHIIAVNSQEQAIFHAEKQKDAITNEIIEL
                     ESSLEELNAEQLEMADKIRELEGQYQASELQFKRFINEKDTWNSVLVEQNKQVESVRA
                     LLHQSELEYDREVTKIQQITDRISREKERLQRAQMRLEELTQHYLQSVAPEAEFKNQL
                     AALLIKHGEIESMLTLSREKVSQLKLQLEDQEKLILNYDQEVKRIQELMNQIQMEKQA
                     LSVRASSIQETLDEANLQAQSLLERIPSGVTQSIKEDELIAILEKIKRLGAINLAAIE
                     EFAAEQQRKVFLDEQYNDLSQALSTLETAIEKMDNETKSRLENTFDEVNTSFKALFPR
                     LFGGGRAQLELTCDNLLEAGIVVMAQPPGKRNSSIHLLSGGEKAMTAVALVFAIFQLN
                     PSPFCMLDEVDAPLDDLNVSRFCDLVKEMSQFIQFLFITHNKVTMELAEHLVGVTMRE
                     PGVSRLVAVDVKQALTME"
     gene            35687..36367
                     /locus_tag="VAH76_000155"
     CDS             35687..36367
                     /locus_tag="VAH76_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEO1384818.1"
                     /translation="MKQQFYKLPQILISLCLLLILSTPIQAEPPNLSLIRKEIKNYYD
                     SGLYYHELERTIKLAQEYIHQQYLINKNNKHPQKLAIVLDIDETSLSNYDKMVKRDFT
                     GSKEQIHKEILAANSPAIKPMLTLYKNALKQGIKVFFVTGRQESERDATRANLIKAGY
                     TKWAGLYLRPNGYSSPSIIPFKSKAREMIAKKGYTIIASIGDQCSDIQGGYAKKGFKL
                     PNPFYYLP"
     gene            complement(36469..37530)
                     /locus_tag="VAH76_000156"
     CDS             complement(36469..37530)
                     /locus_tag="VAH76_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEO1384819.1"
                     /translation="MSKLINLAVLSFSSSLAFAGPLTLKGEVDVHITPKNPSGVAQSL
                     TFKLPKYELSTEAKSYLREQLSEYPKNSINTASFSSELPRKVKLGMQLTPVLDQGYHG
                     SCVTFAVTAAIDAALGAGDYISQLCNLELGSYLAIHDKAKASGWNGSFGYWVLQQISE
                     YGIISQNYQKLNGCAGVREYPLEDENNEGKPMSDSEFLAHSVPVSNLISWEALLKDEE
                     SFSAKADMNQIVYQIKEELAKGNRLTIGMLLDVFVGDAGAVGTNRAYNDTWMLTPEIV
                     LDAMNGMIYAGHELVITGYDDDLEVMDEEGHVNKGVFTLRNSWSKFAGDQGDYYVTYD
                     YVKFLAMEVMAIRMKEKAA"
     gene            37840..38652
                     /locus_tag="VAH76_000157"
     CDS             37840..38652
                     /locus_tag="VAH76_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HEO1384820.1"
                     /translation="MRTLLSCFLITLLSFGLIINEASAKRFGGGRSFGVQRSQSSLFS
                     SNKTHNTSSLTQKANKSRWGGVLGGLLVGGLLASLFMGNGLANGLITWLILGSVIFFV
                     VSFLRRKMNPGFQATQSGAFRQNSFEQFTQNYGNRNSGGAHFAQQETSFDADNFLRDA
                     KVTFIRLQAAYDQKNLQDLQTFTVPEVFAEIKMQLDERGDAPNQTEVISLDAKLLDVS
                     KQSLSTIASVQFTGTIKENGEISNLDEIWHFRQFDNRKEWVVGGIQQEVFQP"
     gene            complement(38726..39208)
                     /gene="greA"
                     /locus_tag="VAH76_000158"
     CDS             complement(38726..39208)
                     /gene="greA"
                     /locus_tag="VAH76_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO1384821.1"
                     /translation="MSKHPMTVEGAEALKEELHRLKFVDRPRIVEAIATARAHGDLKE
                     NAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSE
                     LTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
                     "
     gene            complement(39217..42420)
                     /gene="carB"
                     /locus_tag="VAH76_000159"
     CDS             complement(39217..42420)
                     /gene="carB"
                     /locus_tag="VAH76_000159"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO1384822.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGTQAVRALKEEGYRV
                     ILVNSNPATIMTDPELADATYIEPVQWKEVARIIEIERPDALLPTMGGQTALNCALDL
                     VREGVLAKYSVEMIGATREAIDRAEDREKFRQLMIKIGLDMPRSAIAHSLEEAIQVQA
                     RLGFPAIIRPSFTMGGSGGGIAYNREEFEEICIRGLELSPTRELLIDESVLGWKEYEM
                     EVVRDKNDNCIIVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKVLRAVG
                     VDTGGSNVQFAINPEDGRMLVVEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPSIDYVVTKVPRFNFDKFPQTPDTLTTQMKSVGEVMAIGSNFQ
                     ESLQKAIRGLEIGRCGLHSLFQKEDMARLRGHLREPTPDRLWYIADAFRQNLSLEEIH
                     LESRVDPWFLAQIQELVLLERSIIGKTIRDLDKTTLHLMKHRGFADAYLAKLLGCTEA
                     EVRARRVELEILPVYKRIDSCAGEFPSDTAYMYSCYEGSCEARPESNKKKIMILGGGP
                     NRIGQGIEFDYCCVHAALSLRSAGYQTIMVNCNPETVSTDFDTSDRLYFEPVTLEDVL
                     SIVAMEQPEGVIVHYGGQTPLKLARDLEANGVKIIGTSSDAIDMAEDRERFQQMVKEL
                     KLHQPDNGTVRSEAEAIELANKIGYPLVVRPSYVLGGRAMEIVYQEEDLRYYLSHAVE
                     VSNDSPVLLDKFLNDAIEVDIDAVCDGKDVLIGAIMEHIEQAGIHSGDSACTLPPFSL
                     GVVVQQDLIEQIRKMALKLGVVGLINAQFAIQADDIYVLEVNPRASRTVPFVSKATGL
                     PLAKIAALCKVGISLKDQGLNQNHHAPSFYSIKLPVFPFIKFSGVDSILGPEMKSTGE
                     VMGIAKRFGQAYAKAQLGAGCHIAKRRRAFVSVRDADKTRVGEIAKRLIELGFEIIAT
                     RGTALALQAAGVDCRRVFKVAEGRPHVLDFIKNNEIDFIVNTTEGKQAIADSFAIRRN
                     ALQHKVSYTTTLSGAEAACLAMKYEDRETVTRLQDLH"
     gene            complement(42549..42821)
                     /locus_tag="VAH76_000160"
     CDS             complement(42549..42821)
                     /locus_tag="VAH76_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384823.1"
                     /translation="MNEMDILSLFYDEMIARGVTREQVFLSIEEDAAAMLTQKLGKPV
                     SVEEAQKLTDICIANEWLERTTADPYYKYLSLTEAGLQILLSNLYK"
     gene            42934..44115
                     /locus_tag="VAH76_000161"
     CDS             42934..44115
                     /locus_tag="VAH76_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO1384824.1"
                     /translation="MQEIDVLIIGAGAAGLMCAIEASKRKRKVFVLDHANKAGKKILM
                     SGGGRCNFTNYYIEPNKYFSHNPHFFKSALSRYTQWDFIELVNKHKIPFHEKTLGQLF
                     CDNKSKDIVDMLLKECEQYGVAIYLNTVIEKIQKTNDYSFKIGTTKGKFHCHSLVIAT
                     GGLSIPTMGASPFAYKVAEQFAIKVWPTRAGLVPFTLDVLEKDRLSILSGIGIDSLVN
                     NERNQFREHILFTHRGLSGPAILQLSSYWNPGESICINLLPEHNLLESLKTARAEAPH
                     KQLNSVLSMYLPKRVVEVFIPQKLGEKKLADSSNKDLETISHLLQNWVVKPNGTEGYR
                     TAEVTIGGVDCHAISSKTMEANNVPGLYFIGEALDVTGWLGGYNFQWAWSSGWAAGQV
                     V"
     gene            complement(44150..44902)
                     /locus_tag="VAH76_000162"
     CDS             complement(44150..44902)
                     /locus_tag="VAH76_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2628 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384825.1"
                     /translation="MAFIRHLLEYLLHPFFLCLLTLGICIFLLYRRLAPTFTKVMLFV
                     VLLVLLAMSTGWLPSYLTYQLESRYPIVKRVNPNVKWVVVLGGGQNIGEEMPANDLLT
                     SASIKRLIEGVRLFRTLPDAKLLLSGGGSAGEQSEALLLTELAKWFAIDSEKVVVETA
                     SINTADQAHELAAIVHDQPFYLVTSAIHMPRSMALCQQQGLHPIAAPTDFTFFWSTDT
                     WVKMVIPNSYNLAYTSIALHEILGKIWALGFR"
     gene            complement(44902..45972)
                     /gene="lptG"
                     /locus_tag="VAH76_000163"
     CDS             complement(44902..45972)
                     /gene="lptG"
                     /locus_tag="VAH76_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEO1384826.1"
                     /translation="MSRKILDRYIAKTVLSAIGLVTLMLVGLQIFILFVNQIDDLGRA
                     DYGIVQAGVFVLLQMPYQVYLFFPIASLLGCLVGLGVLANHSELVVMRAAGISIGQIT
                     GAVLKASLIVILLITSLGETVVPVMAHYANEYKVSALTGGQALLTPRGVWVRNGNDFV
                     SIASVTRENTLRYVNQFRFDNQHNLKLARQISEIKYVNNSWIAYDVKQTEFAEDHTKA
                     YHFDTLPWDVPVKPEILKITGTNPDEMTLHELNRYLREQQRNHQNVANYKFAFFQRIT
                     QPLNTMVMMILAIPFIFGPLRSSTMGSKLLVGAMVGFGFHILNRFFGPVSTVFQWPPE
                     LAAFSPTLIFSLIGIYLMRRVR"
     gene            complement(45969..47048)
                     /gene="lptF"
                     /locus_tag="VAH76_000164"
     CDS             complement(45969..47048)
                     /gene="lptF"
                     /locus_tag="VAH76_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEO1384827.1"
                     /translation="MIIFRYLTKEVFLTLIALTAILVLIFMSNQFIQYLNRAASGSIP
                     GFVIMKLLMLELPNLMGLLLPLGFYVALLLAYGRLYAESEMTVLRACGYGPRQLLKHS
                     FAMATVVLVVVAIVMIWASPVIAVERTKLIRSTGIKTLIQTIMPGRFHAINSGQQIFY
                     VQSMNRDHSQAEQIFMAKHSVQDNKLLWDVLWADKAFAEVNPQTGEEYIVLQNGKEYQ
                     GVPGHADYQIAEFASYKSKLPNPTVVMNDDARTAKTRDLWPLNNHDLKKAAELQWRIS
                     IPLMVLTLTLVAVPLSRVSPRSGKYSKLLPAIIIYIFYANFMFVSRDAIATGKIPWWI
                     GMWWVHVAVAILGILFIWRNQVKLA"
     gene            47252..48703
                     /locus_tag="VAH76_000165"
     CDS             47252..48703
                     /locus_tag="VAH76_000165"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEO1384828.1"
                     /translation="MNYGLTHTPSLTMSECLVLGVFSDLALPDFATAIDNEQKGLIKK
                     LCQRVPEPGNTVWQTDVEGHSLLIIQCGKKEEFSANSLQKRVGEITEALIKQRFSSAT
                     VCLPRLNQESAEWQLEQMIVQIDNLRYQLLDFKTKHAKSHKLESVIFHLPGATEKSLE
                     MAKAIVTGVEFCRDLANMPANICTPTYLGEQAISLSKQFDQISCQVMGPEEIKEMGMG
                     ALLAVAQGSDQPPRLIDIHYHGNKNSTPVILVGKGITFDSGGLSIKPANAMDEMKYDM
                     SGAASVLGVIKACALLKLPINLIGIIASAENLISGSAVKSGDIVTTMSGQTVEIINTD
                     AEGRLVLADALTYAERYNPDFVIDIATLTGAIIVALGNIATGYMTRDEQLAKSIERAA
                     NESQDKVWRMPLDEAYQDALESPLADMINAGFDRSAGSITAACFLSRFTEKYRWAHLD
                     IAGTAWISGKKRNATGRPVPLLIQLLRHVANSR"
     gene            48684..49118
                     /locus_tag="VAH76_000166"
     CDS             48684..49118
                     /locus_tag="VAH76_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEO1384829.1"
                     /translation="MSPIRVDFYLLASSLPESRWLVACRLIEKAYIKGHTIFVFCDNQ
                     QQAEYLDELLWTFKDDSFIPHNLQGEGPEPPPPVQIGYQKEPRGFTDILLNLSTNIPI
                     FYTRFKRVIELVSNVEHEKEVSRLHYKEYRYKGCALHTHSIE"
     gene            complement(49265..49582)
                     /locus_tag="VAH76_000167"
     CDS             complement(49265..49582)
                     /locus_tag="VAH76_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEO1384830.1"
                     /translation="MFVTFSCDAYENITYFENVAKQLLLLMGHGGTIPGAIKSENVPD
                     ALHHLQQGLEKIKSENKLLEDDEDSEPDISLAKRAIPLIHLLQAAEKKDCDVLWSYSK
                     SPG"
     gene            complement(49605..50969)
                     /locus_tag="VAH76_000168"
     CDS             complement(49605..50969)
                     /locus_tag="VAH76_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEO1384831.1"
                     /translation="MQAEVFYKSYGDRIVPVHLISQSQWDEGIENLTPMERNCFSVRQ
                     FKGKLGDYCFILNADGVIEKAFIGSGTGNQESALANAALLLPPGNYQFQDKLSRESGV
                     VWALAQYRFDEYKKYELQPRVLIVEANDRDYILSLANSLFLVRDLINTPTSDMGPESL
                     AHVVEELAKTHKACFKQWIGEELIKDNFPAIHAVGRASALTPRLLSLHWGNEKHPRVT
                     LIGKGVCFDSGGLDIKPSSGMRLMKKDMGGAAHVIGLAQWIMVQRLPIRLQVLIPAVE
                     NAIGPNAFRPGDVLTMRNGLTVEIENTDAEGRLILADALAMACEEKPDLIIDFATLTG
                     AARVAVGTEIAAMFSNHDQLAQDIVAASNKVSDPVWRLPLFSAYEELLNSNIADMSNS
                     SSSSYAGAITAGLFLQRFVTKSIPWVHFDIMAWNAVSKPGKPEGGEAMGLRAVAEYLL
                     QTYG"
     gene            complement(50974..52545)
                     /gene="murJ"
                     /locus_tag="VAH76_000169"
     CDS             complement(50974..52545)
                     /gene="murJ"
                     /locus_tag="VAH76_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEO1384832.1"
                     /translation="MLTTDIMVPKKQSLIRSTSLVSLMTFISRIVGFARDMVLANFFG
                     AQAGMDAFFVAFRIPNFMRRLFAEGAFSQAFVPVLAEYQKTRSAEDVRTFIARISGYL
                     SSILTLVTVVGIVASPVIIFLFAPGFHHDSVRAELATQMLRITFPYLMLISLTAMAGA
                     ILNTYGYFGVPAFTPVLLNISMILAAVYLCPDLPQPVVGLAWGVLIAGIVQLLFQLPF
                     LYQRHLLIRPRVVRDDPGVNKVLKLMIPALFGVSIAQLNLMVDSIFASFLKVGSVSWL
                     YYTDRLTDFPLGVFGVAIATVILPHLSRKHTEQNINQYSRALDWGLRSILLIGIPAGL
                     GLSFFALPLIASCFAYGKFTAYDLLQTQKSLITLAMGVPAFMVVKVLASGFYARQDIS
                     TPVKVGAIAMVVNTLLCFVFVWHLAHAGLTLASALAGYVNCAILLFLLIKRGLFKPSP
                     GWLKYSMQLLIANFAIGVYLYFMSGTATYWLGFSPLMRLSLLLAHVFVTVVIYLLVLG
                     IIGLRPSHFRGQIKD"
     gene            52903..53169
                     /gene="rpsT"
                     /locus_tag="VAH76_000170"
     CDS             52903..53169
                     /gene="rpsT"
                     /locus_tag="VAH76_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO1384833.1"
                     /translation="MANIKSAIKRARQNVKLRQHNASARSMYRTYIKNVLKAVESGDQ
                     EAARAAYTKAQPVIDKAANKGLIHKNKAARIKGRLVARLKAMAA"
     gene            complement(53230..54441)
                     /locus_tag="VAH76_000171"
     CDS             complement(53230..54441)
                     /locus_tag="VAH76_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2637 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384834.1"
                     /translation="MFLTINDLQSQIQLAKKRLYVLAKNWLDTQKEAINSKDKANWLN
                     AFQASTKEAMQEQLKATGFPLIRNAILVNQSEDELQQHRLEQSLSLIHSENFPEQFNS
                     RTVPRVIYVCHSGENNQVIKRLCQLTTLMEQLHTLEELTFEVQTNVHFRGLNLAYRMA
                     RSINKEWRDVEKGRETVEYYAERLQIAEKQYKLASDFCHECLGIYKEQLDARIMYFNE
                     SLNFFVECLAATFSSVKDDRYWLTKFLLPSLPWHSMLNEVATCLFTTNGFTNPTIDSI
                     EVNRSHLKAITEQFAKLMMAPFCSLEKELQGKIEKVENEQKIAGENVYAMSSELDIVI
                     HMQSQRKLAALLFPAQPLSMLGFFQGVAAAMGRDYFYSTFIKGNQSQEPRITDDEKSL
                     IEASNYSNGLH"
BASE COUNT        16937 a        12124 c         9792 g        15652 t
ORIGIN      
        1 cccaacccaa atgaaatagc atgtgtgcta aaattgccga ggtcagtcca tattgccaaa
       61 ataaaaaacc aaagagtatg gactgcgaaa gactcaataa tacaaaagag taaagaaatc
      121 gtctggatgg ggtgcaacct gctgcaatat aggctggaag ttggcctaca gcaaaaataa
      181 atgcactcaa aaatattgaa ataattataa tttctgaatc aatttggctt gtaaacaata
      241 aagcaaaaaa gcacactaca ttcattaatc cccatctggc aattacctct tctacaaccc
      301 ctccataaag gacacaacca tcaagtccta ttttggaccg caattcggac ataatcttga
      361 aacttttttc atcaagtatg gaaccaaaca acccataata cagtatacaa aaaataacca
      421 accctaaaag ggcatataaa aaagtaggta ataaaatcac ttgaaaggaa tccaggctgg
      481 ctttgccttg caaaagacct tccaggagag gatcgcctag tccggtacgt gaagacagga
      541 tggagccagc aaaactcatg acaaatgtca taaataaggt ttgcagcaca acaaaacgac
      601 taaatctttt ttttaatctc tcggtattat cagctagcag aaaatatacc aggcgatgca
      661 tcacgattaa aacaccagga atggataaac aaaacaagac aataattaca ggccaatcaa
      721 ttatcatttt tcatcctttc gcaatacact gtaaaaaaag ccatccatgc catgctcccc
      781 aggcaatatc tgttgaccat gcccagtcca tcttccccag gaccatttgc catcactcac
      841 ttgacaatcc gaatgctctg caacaaatcg ggcgatttgc aactcatttt catcactcat
      901 aatagagcat gtggcataaa ccattaaacc acctggtgca agcagtggcc ataatgaatt
      961 caacatactg gattgtacct gagaaattgc tgaaatctct tcatccgttc gtaataattt
     1021 gatatcagag tggcgtctaa tcactcctgt agcggaacag ggcgcatcca gtaaaatacg
     1081 atcaaatgga tgaccatccc accagcttct gggttccaat acatcacctt gcaccaccgt
     1141 tgcttgcaaa tccaatcgat ttaagttatc tctgacccgc tgtaagcgct gtgggtctaa
     1201 atccaatgca acacaggaag ccaaaccaga ctctttctcc aaaatatgac atgtttttcc
     1261 gccaggagcg caacaagcat ccagtactcg ctgcccgggt tttaaagaca ataaatacgc
     1321 tgctaattga ggtgctgcat cctgaacaga cacagaacct tgcgcaaaac caggaagcgt
     1381 catcacatca caagggattg ccaaagtaat tccatcaggt acaacagggt gtttaaaagc
     1441 ctctattccg gattttttta gtaagtcaag atactcatca actgagtttt tcaacacatt
     1501 gactcgcaaa gtcatgggtg ggtgtctatc attcgcctca gcgatgaatt gccaattgtt
     1561 tggccagtcc ttttgcaggc gttgaagtaa ccaattcggc tgtccataga taaaaactgg
     1621 ttctgctgcc agcttttcca gaatatggtc ttgtcgacgg caaaaattcc ttagcacggc
     1681 attcaccagt ccttttgccc aacttttttt aacctgcccc agtaaagaca ctgtctcctt
     1741 caccacggca taatccggct tattcatata ctgcaattga taaataccta tcaacaagac
     1801 tatccatacc tcattatctt taggtcgctt attgattaaa gtatcagcta tggattgtaa
     1861 acgaaaataa tgacgacaaa atccatagca aatttctttt gtcatgggag aaatctcttg
     1921 cggagacatc aaataggaca ggttttttct gctttctatc aaggccgtca aaatttttaa
     1981 ggcgttgaat ctgtcattta ctttcattgc aacattaaac cagtatgcaa ttgtgtttta
     2041 cccgaattta accaatccct aactgaaatg acctttgctc cgggaaattg aattttttcc
     2101 actaacaagg cattatcatt ggtagcgacc aacattccat ttttatcgac atttaatacc
     2161 atgcccggca tctcggtact catgatatca gttgcttttg cctggtgaat tctgagcatt
     2221 aattccccag ccagcgtata ggcaacaggc catggattaa atgcgcgaat ttttctatct
     2281 atttccactg cagactgatg ccagttaatt cgtgcctctt ctttattaat tttaccagca
     2341 taagtcgcta actcattgtt ttgtaattca aattgtgctg aattggatgc taaagcctcc
     2401 aaaacatcca ataatggttt tactgacatt tttgctaatt tgtcatgcaa actaccagcg
     2461 gtatccgagg aagtaacagg acaggtggcc ttgcataaca taggacctgt atccaatcca
     2521 acatccattt gcataatggt cactccggat tcagcatcac catgtaaaat ggcatgttgt
     2581 attggagatg cgccacgcca gcgcggtaac agagaagcat gcacgttaat acaacccaaa
     2641 cgaggaattt ccaacaccgc ttttggtaat attaatccat aggctatcac gaccattaca
     2701 tcaggcttta aggcagaaag ttcatcgata gcttcttgat ttttaaaatt aagaggttga
     2761 taaactggta cctggttatt tattgcccat tcttttacag gagactcctg taatttacgg
     2821 cctcggccag ccggcctgtc gggctgagta taaacagcct tgagatgatg ccttgattga
     2881 attaaagcat ccagacagga taagccaaat tctggagttc cagcaaaaac gacagttaaa
     2941 ccactcatgg tttacgagct tgtaaacgct tgaacttatc taatttccgg cgagccatca
     3001 ttctcttcaa tggagacaac atatcgacaa acaatttgcc attcatatgg tcaatttcat
     3061 gttgtaaaca ctcggcaagc agcccttcac ctgttatttc aaacggtttg ccgaacctgt
     3121 ccagtgcctt cacggtcact ttttcagctc tgacaacggt atcataagcc cctgggacag
     3181 acaaacagcc ttcttcaaat tctttttcgc catgagagga aactatttca ggattaacaa
     3241 taacgatctg ctccttttta tctcccacta tatcaatgac agataaacgc aagctgactc
     3301 ctatttgcgg tgcggcaagc ccaactcccc gagcatcata cattgtatca aacatatcgt
     3361 tgattaatgt ctgtaaactc tcatcaaaag tctcgaccgg cttagctatt tttcttaacc
     3421 gctcatcagg taaataaaga atcttgcgaa ttgccattgt ttatctagtt ctccacacgt
     3481 taatcgtgat attatccccg atatacagta atgctgcaag atgctaaggc tcaactaaag
     3541 ggattgatac atgaaatatt gtctcctcat tttctgtttc attatatcat ccattactca
     3601 tgccttaagc ctaagaccgg attccccctc tcgttatgtt gtgcaacctg gagacacctt
     3661 atggagtatc tccagccgtt ttttgaataa cccctgggaa tggaaagcgt tatggcgcgc
     3721 aaacccccag attcaaaatc cagacaggtt atatcctggt gccgttctcg ctttagagta
     3781 ttatcaaaac attccctatc tcagggttct gtctaacgga acaatcaaac tttcccctaa
     3841 cataagagtg acaccccagg aagacgccgt tccaccaatt ccattaggcg atatcaaacc
     3901 ttttttggat gaatcgttaa tcctggatgt taatgtccta aaccgagcac cgtatgtggt
     3961 agcactcatg agtgagcgga tgcttggagg ccagggagat gaaatttatg tcagaggctt
     4021 gcatccctcc aaagaaatgc ctcaaggagg cactatagga tactccatct tcaggggagg
     4081 gagaaattat tttgatccaa taacccatga attgttaggc tataaagcag tcttggtcgg
     4141 ttatggagaa ttggttgctg gcggcgagcc cgctacggtt ttattgacca gtattaatca
     4201 gggcatcaaa atcaatgata aggtattgat taataaccac cctgaatttg aattgtattt
     4261 cgaacccgaa actcctgcca ggcaagtcag aggatacatt attgaaatgc ctgacaatat
     4321 gcctgttgga aatacacaag aggctgttgg aggagtcatc atcattaatc ttggagaaac
     4381 ctccgggata aaaccgggag atgttttggc tatctatggc aaggaacgta ttgtcaatga
     4441 tccccaaaat catctcagac ctattacctt gccgcaagaa cgtcttggcg aagctatggt
     4501 gtttcgtgtg tttacaaaag caagttatgc cctgatagta agatccacgc gcgctattca
     4561 tttattagat acagtaacga acccatgaat aatctgcaac ccctcctcgc cttaaacagg
     4621 atgaaaaaaa ttggcccccg aaccgtatta aaactgcaaa aaagatggcc tgatttaaac
     4681 cttatgttcc aactgtccag tgttgatctg gaagaagcgg gcttgccatc ctggttggca
     4741 caaaccataa aaaattttga tctggacttt ataaaaactg atttggactg gttaagttca
     4801 tcagaaaatc atcatatttt aacttgggac tccccctatt atcccgcatt gctcaaagaa
     4861 atagccgatc ctcccttcat tttatacgct aaaggggagc tgtccgcctt gacacaaccc
     4921 aacctggcta ttgttggaag tcgcaatgct tctgtaacag gaaacgagaa tgcccgagca
     4981 ttctccaggg aaatttccag gcatggggta agcatagtga gcggtttagc cttgggaatt
     5041 gatgctcatg ctcatttagg ttgtctggaa ggaggcggca aaacgatagc ggtcttaggt
     5101 accggaattg attgtatcta tccgcgccgg catttaaaat tagctgagca aattacagag
     5161 aatggtttat tattaagcga atttccttta aaaagtccac caattgctgg acattttcca
     5221 ctcagaaatc gtataataag cggtctatca ttatgcattt tggttatcga ggcagcaatt
     5281 aaaagcggtt ctttaattac agcaagaatg gctctggaac aaaacagaga tgtattggct
     5341 attcccggat cgattcataa cccgttagcc aggggctgcc actacttatt gcagcagggt
     5401 gcgaaattag taacttcagt ggctgatgtt cttgatgagt taaaaattga gcaccatcag
     5461 ttcgcttcta ataaaccaat tttttccctt gccagcggta aggaaaacct agtaaagttc
     5521 attggttttg aaactacgac tatcgatcaa attattggtc gtagcggata tagcatggaa
     5581 caagtcacga gtggactggc tgaactagaa ttgaaagggg ctgtgatggc agttcccggt
     5641 ggctatataa ggtgtgaata tgaaagataa tttatttgaa atgttattga atttgtttga
     5701 aaaaagtctt actcaactcc aaaaaagcca taaatccaat gatcaagact cccaaagtca
     5761 attgcttgaa gaagatcttt taagtgctga agagcaatca ttgtttatca aatctgctca
     5821 acaaaaatca acacgtattt ttacttacga tgagcaaatg aaactgacaa aagccagcta
     5881 tcaatttcta atgcgtatga aactatggaa tatccttgat atcgatcaat ttgaatcaat
     5941 aattaatcaa ctacaattct ctgaatctcg tattgtaacc ttgcaagaaa caaaatggac
     6001 tataagaaat gctctggcat cctctttgaa tgaagagcaa ttaacttttc ttgatcttgt
     6061 gctataccaa acagaagatg aattaacaat tcactaatta atgagtgtac tgatatcaac
     6121 ccattcatga actgttagtg agccacacaa cccaaaaagg ataaggttta tttgatatga
     6181 gtaaacactt ggtgatcgtc gagtcgcccg cgaaagctaa aacaatacag aaatatttag
     6241 gaaatgacta tgacgtgtta gcgtcttatg gtcatgtgcg tgatctgcct gctcgtaaag
     6301 ggtcagtaaa ccctgaaaaa catttttcca tgacttatgt tcctattgaa aaaaatgcga
     6361 gacacattga tacgatagca aaaactctta aaaaatcaga ctctctactg ctggcaactg
     6421 accctgatag agagggagaa gctatctcgt ggcatatttt tgaattaatg aaagaacgaa
     6481 accttttaaa ggacaaatca gttcatcgaa tcttttttaa tgaaattaca aaagcagcaa
     6541 tccaggatgc aattaatcat cctcgctcca tatccatgga tttggtgaat gctcaacaag
     6601 cccgacgcgc tcttgattat ctggttggct ttaacctgtc ccctttgcta tggaaaaaga
     6661 ttcggcgagg attatcggcc ggacgagtac aaagccctgc ccttaggtta attgttgagc
     6721 gagaagagga aattgaacgt ttcattgctc aggaatactg gaaaataatc gcaaaatgcg
     6781 ctcatgccag caccgaattt gaagcacgac tgacccatta taacgaagag aaacttcaac
     6841 agttttcagt cactcaacaa gaacaagccc atgaaataaa aaagcaactg atagcgcaag
     6901 cacaaggctt tttaaccgtt gctcaaattg ataaaaaaca gcgtaaacgt aagccatctc
     6961 ccccctttat tacctctacc ttgcaacagg aagccgccag aaaattaggt ttcaccgcac
     7021 gaaaaaccat gatggtcgct cagcaattat atgaaggtat tgatataggc acaggaacgg
     7081 tgggtttaat cacttacatg cgtactgatt cagtcaacct ggcaaaggaa gcgattgacg
     7141 aaatacgcga ctatatcaca caacgttata agggagataa ttgcccaaat tctcccagaa
     7201 tttacaaaac taaatccaaa aatgcacagg aagcacatga agccattagg ccaacttcca
     7261 tcaaaagaac tccagaaatg gtgcaaggtt ccctgacaag cgatcaattg aaactctata
     7321 gcttaatctg gaaacgcaca gtggcctccc agatggctga tgccatcctg gatacggtga
     7381 gcgtggattt tagctgtggt aaagggaata ccttcagagc caatggttca acaatagcct
     7441 ttcctggctt cctttctgtc tatgaagaag gacgagatga ttctaaagac gaagacaatg
     7501 aagataaaat tttacctgct ttcaatgtag gtgaaaaaat aaaagtatcg gatattgaaa
     7561 ccaatcagca ttttaccgag ccacctccga gatattctga agcaactttg gttaaagctc
     7621 ttgaagaata cgatataggc agaccatcta cttatgcctc tatcattcat actttgcaac
     7681 agcgcgaata cgtggtagtt gaaaaaaaac gttttttgcc cactgacgtg ggtagaattg
     7741 tcaatcgatt tctaaccaac tattttactc gctatgtcga ctatcagttt actgctggcc
     7801 ttgaagacac actggatgcc atcgcgcgtg gagaaaagga ttggatccct gttctggaag
     7861 agttctggca acctttcgtc cagcaaattc agaatataga tgaacaagtg caaagaaagg
     7921 atgttactac ggaattatta gaagaaaaat gtcctaaatg tcaaaagcca ttgtccatta
     7981 ggctcggcaa gaggggacgc tttattggct gcacaggata tccggattgc gattacactc
     8041 aggatataag caatccagaa ggagaaaaat cagaacctga agtcgtggaa ggtagaagct
     8101 gccccctgtg ccatggagcc ttgcatatta aaaccggtcg ctatggaaaa tttattggat
     8161 gcagcaatta tcctgaatgc aagcatatgg aacctctaga aaaaccctcc gatactggcg
     8221 tcacatgccc taaatgcaac caggcgaaaa ttctgcagcg gaaatcaaga aaagggaaaa
     8281 ttttttattc ctgtggaaat tacccaaaat gcgattacgc tctatggaat gaacctgttg
     8341 atttgccttg tcctaaatgt gcatggccaa tcttaacctt aaaagaaagt aaaaaatttg
     8401 gtcgtcaaat tctttgtcct cgcgagggtt gtgattactc tgctaaagaa gattaaaggt
     8461 aaacgttcat ggggcatgta aattccgtca ttgcgaacga agtgaagcaa tccagtacag
     8521 aattctaaca aaatatcgtt ctggattgct tcacttcgtt cgcaatgaca gcatttatct
     8581 catttgtttt tcaagagatg gcataacccg tgaacgttta ctaatctgtt tgttttattc
     8641 gcaatccaat aatccagtaa attatctcaa ctcactaagg gattgttatt cttctgtaag
     8701 tataatttgg ccaaatgata ggaagcataa tcggtgatat gtcctgaatc gcgaaacact
     8761 ggaataccat tgatatctac cttgcacatc cccgacggac actgagctac tcgtgggtca
     8821 ataatgacca gtgtcttgta ttttctttgc atttttgcaa acaaattatc gaaccaaatc
     8881 tgttcactga catccaggtt aaaatcacac cgctcgggat tgtatttttt atgcagcttg
     8941 atatgctcaa aaaaacaatt gtaaggattt cccttagaca gtgctatcga tttgattaat
     9001 acaggtttcg ctcctgaagt ggtaataagc tccagagctt tatccaaagc cttttcaatg
     9061 cgctttaaag aaagctcttg agatcgattg tcattcaatt gattaataat tctatcacca
     9121 aaataaccat tccagttttg gcctaaaata acataatcat agtggttggt tttaatcata
     9181 ttaaaatagc gattcgtttg atcatggcat tcctggtaaa tcttattttt aacattccaa
     9241 tcaaattgat atatgtcagg caaagcaaga caagcaaccg ttgcatgagc caaaatggat
     9301 acattggcct cctttgccaa tgtatccata aatccccaat gctgattgga ataagaatcg
     9361 cctatcataa agccggttcg actatccgag ttttttgctc ccagaacgca ctgaggatta
     9421 acttcggtat ttttgcgttc cagacacaaa ggcctctgtg gatttttgta cttgtttaat
     9481 tccgcataaa cctggattgc ttcattgaaa cgatgaggat agcccgcatt ctttttaatc
     9541 agataatcac tcaagtgagt gacaaaaacc ggcataatca ataaacaaac cactgtatag
     9601 ctgaaccgga ttcgatgcaa tttccttgct ggtttttcaa taaatcgcca ggaaagatag
     9661 gcgataatca atatcagccc aaaagtaacc aacagaacca aattggactc ttcaatgttt
     9721 aaatagcgca ttaaagccaa tacaggccaa tgccaaatgt acaaggaata ggaaattaat
     9781 cctatgaaca ccagaggtct taatgaaaga gcataagtta ctatagattg cgactcttgt
     9841 tgtccggcgg caatcagtat ggccgttgca aggcataaaa ttaaagtata ccaattggga
     9901 aaacctatat taatgtcatg atgcattgca atataaaaca gcgtcagaac agccaagaca
     9961 gttataaaat ttaatacata cttattggct aaaaagcgtt tactccctga agctgaggca
    10021 acacaggaac caattaaaaa ctcaaagata cgactggaaa attgataata cgtttttgca
    10081 ggattactcc aagaataata catggataat gcgaaaaaag tgatggttaa taaataggtt
    10141 atcttgattc gatgctgtgg accgaccaat cgataaagca aatagattcc aaccggcaag
    10201 ataaaataac attgccattc tacggataaa gaccacatat gcaaaagagg caattgatta
    10261 ctatccggag aaaaataatc gctcgtgaca tttttaaaaa acacattcga tgtaaaaata
    10321 gatgttttac gggcactttt actaaaaagc atcaagtcat cgggcagata ataaaacagc
    10381 gtaaccaagg ttgtaactac aatcaaacaa ataaaaacag gctgtaatct ccataatcgc
    10441 ctgctgtaaa agtccataaa agaaaaacga ttattttgca aagatttttc aatgatacta
    10501 gtaatgagaa atcctgaaat gacaaagaaa acatcaactc caataaaccc ggatgggaaa
    10561 agagttaagc ctccatgaaa aaataaaaca aataaaatag caattgctct taagccatca
    10621 atatctgagc gatagttcac actacaattt ccataaaata attgttgatt aatactaatt
    10681 acatgcccac ccttgcactt tatttctgta caaaggtttg caaattggga aattcagagt
    10741 tatatcacaa taatcttttt ttagtgagaa ctattttagt gtggttcatt catcaattgt
    10801 tcaatgtagc gtctgcaggt taacggttta tataaataat taattaaata gcgagttcca
    10861 ccagttcctg tgattatcaa agtaccataa cttaaaatac tgcctaaaat ggattgacgt
    10921 atgtctatcg tttcaatttt attcaggggg atatccacag tttgccttac tatgataccg
    10981 gtacgcaaaa taacttgctt cctttttatg gtaatggaag aaaaatgata ggttacccag
    11041 gtaatcaata tccagatcag tgcaaaaaat cctaaaaaaa gactcacttc gcgcaactgg
    11101 atgatataaa tataaatcgc catcactaaa cccaatccga cgaaaggacc aaaaaatatg
    11161 atccaatgca atctggtaaa ataaatgaca ttactttctg tagtattttc agccattgat
    11221 cacccttata tacctcaaaa aaatatgata ttgcacttaa cgttaatttg ctattctata
    11281 cataaatcaa cttatttttc gatagtctaa ctctttaata gatagctaac aattaagacg
    11341 gacgttatga aatcaatcag tcaatgccta aacaagcaac tcagtacaat ttgccagcat
    11401 tccatgcaat tggaagagtt atctcttaaa atttctgcat ttcttccttc ccatttcatt
    11461 tctaaatgtc aagtaggcag ttttaataaa gggtgtttgg ttttaaccac aaccaatgct
    11521 gcttttgcgt ccgaaatacg ttatttactt cctgaattac gggataaatt gagaaaagaa
    11581 gccggccttt atcagctgtc ttcaattaaa attaccatca ttgctccctc ctctcaaatc
    11641 gaaaaccctg cacaaaaaaa atctcttacc ctgtctgaaa aagccaaggc aaccattatt
    11701 agtgaaagcc aacactgtac ttatgagcca ctgaaaaagg cattactgca tttagctcaa
    11761 agtgaataac tcatcaacca tctcatttgt gttctgaatt ttttttcact ataatgctca
    11821 aatattcaac taatatgtta gagcttgcta tgcctaaaat aaataaaatt gttaatggaa
    11881 cagacctaac cccccattat ctgagtgagc caaacaaaga attcaaaatc taccgttata
    11941 ataaggaagt ttatgctgtt cgttttgaga atgatgaacc catggattat gttttaatgt
    12001 ggaaaagtca caagagtggg catacgcaga acagtgaaat gattaaaaat accgaagagg
    12061 attataagga gcttggaaaa ggagaacaag gaacggtata tgagaaaaca gaagataagg
    12121 ctatgaaagt atctcgaggc agacatccaa gagaatttta tgaagaaatt aacctccata
    12181 ttatagaaca acaatttttc cttaaatatc atggcattca agagcacttt gttctaggac
    12241 tctggaatat caaaaatgaa gaaaacgtgt atttctacat gcctaaaatc aatgcgatcc
    12301 ctattaacaa aaaaatcgac cagcccaaaa ttgaggaatt tgttcttgcg cttaaagaac
    12361 ttaatgatgc agggtactgg catcctgatt tggcaaataa tccttaccat attagtccac
    12421 aaaatctgat tgcaacagaa gaaatggtaa agactattga tcttgatggt ggctttcggt
    12481 acgacaaggg aagagttgat gaactcagca ggaagtctct tgtttatggt aaagatcaat
    12541 ggctatacgt ctataatttc atctatccac ccaccgatga ggaggataac agaattgatt
    12601 ggagagtccc tatagagaaa tggtatgaaa ataacagaga cgaatcttta tcggttaatc
    12661 cccatgcgtt gcttcgattt taccatgagg gacttatatc attgccaaaa aaacttgccc
    12721 atgatctgca tgagacaata ttagaagagt tatcccagga taaaaaattt aaaaaacatt
    12781 cgcttgaggt aggggtgaat cgctttttta gcccgaaaaa acaaggaatc tatgagggtt
    12841 taaagggtga tgggctaaaa aaagtgatcc tgaaagaact tagagactcg ttagctgaga
    12901 taagaacaat ggagcaattg gaggaaaaaa aactggagtt tttagcttcc ccagaaatgc
    12961 aaattcttgc tgaaggtcag gataagacaa caaaagcact gaatttaaaa accagttctc
    13021 gtaaaaaggt gatggtaata tttaaagaag cagaagagcg aatattgaat agccctaatg
    13081 taagcattta ggacgtgtca attttaaatt ctgtgaatag catgctttta tgtgagttag
    13141 tttcggctta attcaaataa ctcttgaaaa attaattaag cctttttcat ggcaaactta
    13201 tagcgtttcg acaatttttt taagtaaagc tccattgata acttccccat aaaaatgggc
    13261 cctcactatt ttcttctttc gctaacattt cttttgaggc aattcgcctg tcatcgatat
    13321 tggcttccaa tcgattaagg tgcattataa tcaaattaat acaatgtatt taaatctttc
    13381 ttaaggtgat catgctgaaa caaattgtgc taaccggcct agttattata gggattgtca
    13441 ttacgctaat gtacttattt cagcgccacc tcatttattt ccctaataga cataccccaa
    13501 agttggaaga ttacaatgca tcggatatga aagtagtttc cttacgtaca aaagataatt
    13561 tacatttaaa atcctggtat aaacctgcaa gcaagcatcg ccctacaata ctctatttgc
    13621 atgggaatgc tgggcatatt ggttacagaa tgcctttggt tcgtgaattt attgacgctg
    13681 gtttaggcgt atttttatta gaatatcgtg gttatggagg caacccaggc aaacctagcg
    13741 agaaaggtct ttatgcagat ggagaaacag ccattgaatt tttaattcaa catggtgtgc
    13801 catcaaagcg cgtgattcta tatggggaat ccataggtac cggagtagca actcatcttg
    13861 ccacaaaata cccggtttgt gcggttatgc ttcaatcacc ttttacatca ctaaccaggc
    13921 ttgcccagta tcactaccca ttgaacttcc ttaaaccctg ggatcaatat aattcacttg
    13981 cccgtatgaa aaaaattaat gcccccattc tggttttgca tggaaaattg gatcagatag
    14041 taccgtatca agagggctta aatgttttta atgaagccaa tgaacctaaa aaaatgattt
    14101 cttttgacga caaagaacat aatgatttgt ggagcgctga taatttttcg agggaaatta
    14161 ttcaatttgc tttaaaccag tgttcctagc ttttttggat agtttattgt taaggcattt
    14221 tcatcatcga cgttattatt gaactcaatc atccatttcc gcaaaggcca ttgaaaatct
    14281 cctttcaacc cactccttat tgaggtactg aaagcaatct tgcagataat cggccaattc
    14341 ctcttcatcc aagacatgct caaaacctac aggctcaacg tcatgatgaa agtattcgcc
    14401 gaaatacttc attgagaaat caatataatc ccgagtatgc aaaataaaag aatgccacat
    14461 ctcatcgagg atcaataatg gtccaaaaag ataggttttt ttcccccgta accttctttg
    14521 cttattaagc cacatccagg ctaataaatc ctcaaagaga aatagccctt cttgtatgga
    14581 atattctgga tgagaatagc aaaaatgagt cactattttt tcatttttat agtgaagtaa
    14641 ttcagaaaga gaaaaactca ttaacgtcca caacaagttt ttttattact tggtttctga
    14701 ataatgaatc taattattct acaaatcatt tcttctttag ccataattgt ctcttattgt
    14761 taaaatcatt caagtttaac ccaatgtttg taaagggcgc aactgaatgt ttttattttc
    14821 atcaatcagt atcatatgtt gtggtggcac atcttcccat ccttcatcca aatcatttag
    14881 cctttcagaa gagactacaa cataagctgg attaccttct tcttttatcc ataatccttg
    14941 agtagataat acatacccaa tcaaataatg cagcgtcaaa ggttttttct tctcatgact
    15001 gcaatagcgg gtagcgataa tctttttacc atttgtaaga caaatattaa aataggaagg
    15061 tccactatgg ccaaattgtc ctattacttc caatattttg ttaatagttt cttgcagcac
    15121 atcagcagcc actgatagtt gattaatgtc tctccctttt gctaattgca aaaacaaacc
    15181 aaacaaatgc tcagaatctg tttgaccttt aacccaatgg taaatgtcat catccaacaa
    15241 atgccttata tgccttttca catcaataaa atcattaatt tccccattat gcattaacat
    15301 ccaatcgcca taaataaagg gatggcagtt ataattggtt actcctcctg cactggcaga
    15361 gcgaacatga gcaaaaaaac aaggagactg aatttttgct gttaagtgta aaagatttct
    15421 atcgttccag gctggataaa cggaggtgaa caaagcagga tcagagctaa cttctggtga
    15481 ataccatccc aaaccaaaac catctccatt ggttcgatat ttggtttcgc gcgcatgcag
    15541 actctgcatg atgattgaat tcttaggtct taccaacaca ttttccaata gcgtagggcg
    15601 tcccaaataa gcaacgaatc gacacataac agcctccctc atcgagtgat tcgaatagca
    15661 aactctaatt aattaactaa cttccaactt tctattactc atatccctaa atctgtaatc
    15721 cattcggctg tcattgcgcg tttttataag gtatccggaa tcactgcaaa tcaactcact
    15781 taccatatga tgcaacgatt cttacttatc atagggctac catttggaat attaaatgcc
    15841 tcttgaaatt gaggaatatt tgccaagcta ccattgactc tgtatacagc aggcggatgt
    15901 ggatctgttg tcacttgatt acgaagttgc tccggcctga cattcatagc ccaaacatga
    15961 gctgttccta agaaaaattg ttgatcaggg gtaattcctt ctatcgtttt agcattcttg
    16021 tactcttttg aacggtgaaa cgctttatat gccaagatca ttccgcccag atctgcagtt
    16081 gcttcgccaa caaccaattg tccctgtaca tgcagatcgc catctactac atattttgag
    16141 aattgatcag caatgcattg tgtcgcattt ttgaattttg ataaatcatt tggcgcccac
    16201 caatttttca aatttccata accatcaaat tgagcacctt gatcatcaaa tccatgcgtc
    16261 atttcatgac ctataacata ccctattgct ccataattta tggcagtcgg agcttttgga
    16321 tcaaaaaagg gagattgtaa tatacctgcg ggtatattga ggttattcat tgaaggatca
    16381 taatacgcgt tgatcgtttg aggcgtcatc gcccattcac ttctatctac aggtttacca
    16441 attttgtcga gatctcgatt gatcaagaat tcgtttgctc tgataacatt caatacgtag
    16501 gggccacgat ctattttcaa gctggaataa tcccaccatt tggtagggta accaactcgt
    16561 tcttccatta aatccagctt tttcaatgcc gcatcacgag tcttgggtga catccagctt
    16621 aaagttttta tatcttcttg tagtactgct ctgatatttt ttagtatatc caagaccttt
    16681 tgtttggatt caggagaaaa atatttctct acatacaatt ctccgattgc aaatcccaat
    16741 gcaccatttt cagtgttaac tactcttttc caccttggta atagtttttg tgtaccagtt
    16801 aatgcagaaa ccatcttaaa attctgatcg acaaacggtt tggataaata aggagcgaat
    16861 gcatctatca aatgccaacg cagataaatt ttccattcat ccaagctaac tgactgtaat
    16921 aattcattca tcgctttgaa aaaatcaggc atagcaaaat tgattttttt aattttgccc
    16981 tgacctctag cgctcaagta ttgagaccag gaaaaattag gtgtcaattt atccaattca
    17041 gtaatggtca tcatatgata aatggcgtgt ggatctcgtt gctccacttg tgacatggat
    17101 gctttagcca attgagtttc aatatccatt acaattttag cttcttttgc cgccatttca
    17161 ggactgtcac ccaatagctc aaacatttta gtaagatggt taacataagc ttctcttacc
    17221 tgtttaaatt tagcttcttt tttaagataa taatctctgt ctgggagtcc caatccccct
    17281 tgcataacag cacctatcat ggaagtgctg tccttaaaat cttgcatgct gccaaaatta
    17341 aacaagggcc ctacgcctat tttgtgcaga tgggcaattt cagcctgtaa atctttgaga
    17401 ttcttaattt cttctatttt agtgaattct ggttttagag gcgtgatacc tgacttatta
    17461 atgctatcaa catccatacc gctaaaataa aaatccccca ctttctgctc aatacttcca
    17521 ggtttagcat gagtattctt ggatgcattg attaacatct gatgaataat attttgtact
    17581 ttctcattta caacataaaa acttccccag gaagcatagt cagcgggtat tgggttattc
    17641 tttttccaat tgccattggc ataggtataa aaatccacag ctggtgacac actggtatca
    17701 cgccagtcaa gatgaagaag atcgtttgta ttattatcgg aaaaattgga gttggaagaa
    17761 aatactataa aactagataa catcgtaaca atacaagttg caatcttaaa tttcatcctg
    17821 atattcccct taatttgagc tttatataaa tttctaaaga aggaaactgc ttaggccatt
    17881 atatttagta tagataatgc ctataaaatg ataataatga aaatgaatat tctcactacg
    17941 tggatatgat gtatttctgt caggcttcaa caggataata ttcagggtga accgcgggca
    18001 gataattttc tgtatcaaaa taggtatatt cccaagcatc ctgccttacc attaattcac
    18061 gcaacaatct gttgttcaat tcgtgaccag atttatatcc ttcaaatgcg ccaattaaac
    18121 tggaacccaa taaataaaga tcaccgatag catctaatac tttatgagta acaaattctg
    18181 attcgaagcg gagaccatct tcattaagga cacgataatc atctactaca atagcattat
    18241 ctagactacc gccttttgcc aagtcgcact ctctcaattt ttcataatct gaaagaaaac
    18301 caaaagttct cgctctacaa acttctttga catacgatgt tccagaaaaa tcaaagctta
    18361 ctgtttgtgg tctatcatta aaaaccgggt gctcaaaacc tatggtaaat gttattttat
    18421 atcctttatg aggaaggaac tgaacatact taccattttc ttcgactcgt ataggtttaa
    18481 gaattcggat ataccttttt gctgcatttt gctctcttat acccgctgat tgaattaaaa
    18541 atacaaatgg ggcggcactt ccatccataa ttggaatctc tggagcattg acatctatat
    18601 atgcgttatc aattcctaac ccggccaggg cggataataa atgctccacc gttgcaattt
    18661 tcaccttgcc atgatgaagg gtggtgcaaa gcatagtatc tccgacgtat tcgtaggagg
    18721 caggtatttc gacaactggt gacaaatcca cgcgtctaaa aacgatacct gtgtttacag
    18781 gagctggtct taaggttaaa agcactttct caccggaatg taatccaaca ccagtagcct
    18841 ggattacctt tttaggagtt ctttgtttta tcattcagtg ctcacctttc tctcttatat
    18901 ttattttgca cgcaaaaacc ttaagatctt agcaacaatt tattgattag tctaattttt
    18961 ttaatttcaa ttaatgcggt tttggaaacc caatacaaaa attgcaaaaa ataaccacac
    19021 gaatcaattc atgagatact caagaaagaa cgtttgtatc catcaaatac atccttgttt
    19081 tcaaacacat cctctctcaa gatatcccgt taaaaatgct tctatgcttc ttcttgccta
    19141 cgtaaaaacg ccggtatgtc caggtaatcc acatcaggaa cactctcatt accattattg
    19201 gtttgctttg tagctgttgt tgcagtacca gtctgagcct gcttacgaat caccgccggt
    19261 ctctccagtt gttgataatc aagagaccca tcgcttcttg ttgtttcaac caggcgtgct
    19321 ctaagaggtt gttgtggctg ttgctgacgc tgtctattat cacctaaacc agtcacaatc
    19381 acagtcactc tcatctcgtc tgtcatttca ggatcaataa cagttccaac aactacagtg
    19441 gcatcatcag aaatgaattc tttaaccacg tcacccactt cttcaaattc accaatagac
    19501 atatcaaggc ctgcggtgat attcactaat attccgcgag cgccggagaa attcacatcc
    19561 tccaataaag gagatgcaat cgctgcttcg gccgcttgac gtgccctttg ctcaccaact
    19621 gcgcttcctg ttcccatcat ggccatgccc atttctgaca tcaccgtacg cacatccgca
    19681 aaatcaacgt tgatcaaacc agggcgagta attaaatcag aaataccttt cacagcacca
    19741 agcaacacat tatttgcagc tttgaacgca ttgagcaaac tgatattttt tcctaaaaca
    19801 cttaataatt tattattagg aatagtaatt aacgaatcca cgtgctcagc taagcgtcga
    19861 ataccttcct cagcagcaag cgccctttgt tttccttcaa aagaaaatgg tttagttacc
    19921 acagcgaccg ttaaaatacc taattcctta gcaatttcag caaaaacagg cgcagctcct
    19981 gtccctgttc ctccgcccat tccggctgta ataaagacca tatctgcacc actaagaatc
    20041 tctttaatgt gctctctgtc ttcttctgct gcttctcttc caatttgtgg attagcacct
    20101 gcccccaagc ccttggtcaa tgcatcaccc aattgaatgt gtattttggc actggaacct
    20161 cttaaagctt gtgcatcggt attcgcacaa ataaactcca caccatcaat attttcagcg
    20221 accatatgtt cgacagcgtt accaccgcca ccaccaacac ctacaacctt gatgacagca
    20281 ttattttctt gctggctgct ttccattaat tcaaacataa ccctgtctcc cctaatgtgc
    20341 tgcttttttc ttattaaata aattaaacag ttttgaactt atcgcatttg acactttaca
    20401 agacaaaaat ctgcatttgt tgtcatgcaa aatatcatgc aaccccatat ctcaatttct
    20461 atccatacca ggaacatttt taagcatcat atcagtgttt ttacatgaaa agctaaaaat
    20521 ttccttgaaa ccattctttc atgcgtgacc aaatgctttt accattatca ttcattgctg
    20581 gcacattata gccactttca tattgctgtt gaaagccatg aagcagtaaa ccaacccctg
    20641 ttgcaaacga aggattttct gtcgcttcag tcaaaccaga tacataatgc gcacatcctt
    20701 ttctgactgg catttcaaaa caaagttcgg ccaactcaat tgctcctctt acattggaag
    20761 ctccacctgt tagaacaatg ccagcagcca aacggtcttc aaatccgctt cggcgtaatt
    20821 cattacgtac cagtgaaaat agttcctcgt atcgagctga aacgacatct gccaaagctt
    20881 tggccgagat ttttcgccca ggcctatcat tgacgctggt aacttctaac atttggtttg
    20941 gattggctaa ttctggtaat gcacaagcat gattgacctt aattgcttct gccgccttgg
    21001 ttggcgtacg caaggccatt gcaatatcat ttgttacctg atcacctgca atcggtatga
    21061 ctgaggtatg ttggatagct ccctcacaaa atatcgcaat gtcagtagtg ccaccaccga
    21121 tgtcaattaa acaaacccca agctcttttt catcctcagt aagaacagca tggcttgaag
    21181 ctaattgctc cagaatgata tcaatcacat ccaaaccaca gcgtctgaca cacttcacaa
    21241 tattttgggc agcactgaca gaaccagtaa caatatgcac ccgagattcc agccttacac
    21301 cagccatacc aattggttca cgaatactgc cttgatgatc aatgataaat tcttgcggta
    21361 atatatgaag tatcttttga tctgcaggga ttgccacggc ctttgctgca tcgataaccc
    21421 tttccacatc ggcttgagat acttcctgat cacggatagc cacaatacca tgggaattga
    21481 ggctacgtat atggctgcca gcaatgcctg cataaaccgt cctcacctca caaccagcca
    21541 ttaattcggc ttcttgtaca gcacgttgga ttgaattgac tgtggtttct atatcaacta
    21601 caactcctcg ctttaatcca cgagagggat gacggccaat acctataatt tctatcgttc
    21661 catctgatgt tacttcacca attaatgcaa taatttttga agttccaata tccaaccctg
    21721 tgataatatt tttttctatt tttttagcca ttatcgtccc atttgttgtt tccactgcac
    21781 tgccatgcca cgtggataac gcaggtccac gccagccaac tgatctgctt tttcagcaaa
    21841 aacagcaggg tatgctttac aaaaacgcag caaccgctct tctaactctt tctttcccaa
    21901 gtatattttt acactatgat taagcagcaa cacccaggat tgattatctc ttaaatacaa
    21961 gcctgaagca tttaaaccgt atgatgataa tatcttactc aatttttcat aaacttgtaa
    22021 gacttctaat tgctgagatt gcggaccctt taatttggga atatccaaat cagagggaac
    22081 agaaccctga ttgaataact ttccatccct tgtcattaga gcatcacccc aaatcgctac
    22141 gggctttttt tcagtgagtt taattttcaa tgtatctggc caaactcttt ctacataggc
    22201 cgtatcaatc caactcattg aattcaactc actttgcaat ccttttactg ataataaaaa
    22261 gaaactggca tccagatatt tagctaacac attctccaat tctttatgtg ttatatgttc
    22321 atacgtcgct gcaactttga tcgttgtaat tggaaaacgc tctgcatcag caagatataa
    22381 atagcctaag cgacccgcta gcagcaaagc actaagaatt aacaaaccca ttatcgttaa
    22441 ataacgcaag ccgacagagt tcacttccat aatttagtgt tcccttcaca agtaatttgt
    22501 caccaaatgt tgacgacaat tatgatgaac cattgccaga ttaatcaatt catcaagtaa
    22561 atcaggataa gctacaccag ttgcctgcca caatttgggg tacatactta tggaggtaaa
    22621 tccaggtaat gtatttattt cattaaagta aatttcttct gtcttatcat ttacaaaaaa
    22681 atctacccta gccatgcctt tgcatttaag ataactaaaa atattcgctg cagcctgttt
    22741 taattgttct tccagactat tatttaattg agctggaata atcaaatccg tttgactgct
    22801 ttccaaatat ttcgcagtat aagaataata accgtcaggg tggtttactt ttatctcacc
    22861 caccatactt actcttggtt tcccacaggg aatactgttc tccaacactg ccagctcaat
    22921 ttcccttccg acaataaatt cttcaaccag aatttcttca tcatagcgta atgcatcagc
    22981 aactgctgcg tttagctcat ccatattatt tgctttgtga attcccacac tggaacctag
    23041 tgagcatggt ttaacgaaca gaggccaacc aaactccgag gcaacttcat gacaaaattg
    23101 ttgtctttca gacgcattgg catgccatga taataattta tatcttgctg atttgagccc
    23161 attaatgcat gcgagtcgcc tcgccatgtc tttgtccatt ccgattgcag aagacaatac
    23221 gtcacatcct acataggcga caccggctaa ctcaagtaaa ccttgtaaac atccgtcctc
    23281 atataaaggg ccatgaacca ctggaaaaac aatttcagca tcaactgcca gacgtccatt
    23341 aattaataag ccttccaacg gggttgattt ttcagtaaca acaggtaatt tatcagaaca
    23401 ggcgagcaaa tcattataat catgacgatg aaatcgaccg cttttgtcca ttgcaatggg
    23461 tataatatga tatttctcgg aatccaggtg ttttagaacc gatgcggcag aaaccagtga
    23521 cacttcatgc tcgcctgatt ttccaccata aagtaaaacc aaattaacgg gtttggacat
    23581 tagcgtctcc aatgatatgt acttcgcgta tcaactctat agatgtttgc tctcgaactt
    23641 tagtttgcac caaatgaatc aaggcttcaa tattagctgc cgtggcagta ccttgatgat
    23701 tgataataaa attggcatgt ttttcagaaa caactgcccc accaatgctt acccccttga
    23761 ggccacaaga ttcaataagt cgcgctgcaa aatctccagg agggtttcta aataccgacc
    23821 cacaattata ttcattagtt ggttgggtct tggctctatg agccaataaa tccttaatta
    23881 attgcaagga tgtctcttta ttacccggtg atagctgaag cttggcggaa ataaaccatt
    23941 catctcccaa gccagcaaca tgtcgataag ctacttcaaa ttcttccggt ttacgggttc
    24001 tgatttcacc tcgacgattt atcgtttgta cttcaataac cgactgccag gtttcacctc
    24061 catgacaacc ggcattcatt cgcagagcac cacccatggt accggggata ccagcccaaa
    24121 actctcctcc tgacagatta ttacgcgcag aaaaacgagc catactggca caggataccc
    24181 ctgcctcaac ccgaatacag ttatcgctca atagggtcat ttcttttaaa cagccctgag
    24241 tgagaatcac ggtaccggaa aaaccgccat cgcgaattag agaattagag cctaaaccta
    24301 accataataa tggctcatca aaaggcaatc gacttaaaaa caaagccaaa tcatctatat
    24361 ttgctggttt atagagcctc gctgcagggc caccaactcg ccaggtagta tactcggcca
    24421 aaggctcatt gaaaagcaaa gcgccctgag actctgttac atgactgctg tccattcccg
    24481 ttatgctcat gttcattctc acatgttttt catcaggttc actgccattt ggccgatact
    24541 tccagctccc tgcatcaaaa ttacgtcacc atctttaata aattcatcta aagtggcttt
    24601 caacgattgc tcgctcacta tggtcactcg tttatccctg gatcgtattt cattggccag
    24661 attttcactg gtaacgcctg gaatagctgt ttctcccgct gcatagatat ccatcaataa
    24721 caactcatcc gataaactta aaacatcgac aaattgtcta tgcaaggact gtgttctggt
    24781 ataacgatgg ggttgaaaga catgaaccaa acgtctctcg ggccaaacac gtctaaaagc
    24841 atcaattgtg gataaaattt cctgtggatg atgtccataa tcatccacaa taatcgccgc
    24901 gcctttttca aactgttttt caccaagcat ttgaaagcgg cgtcctaccc cttggaattt
    24961 ttgtaagcca cgaacaatgg aatcgtcatc aactcccaac tcggtggcaa ttgctataga
    25021 agctaatgca tttaatacat tgtgacggcc tggatattgg aattgtatag tcagttgttt
    25081 atgtggtgca ggtcgaacga ctacaaactc actcagcatg cccttttgag tccaattaat
    25141 tgcccgataa tgagcttctt ctttaaaccc ataagttaag gttggtcttt gaatagccgg
    25201 gaggatacgg caaatttctt catcttccaa acaaactact gctaatccat aaaaaggcaa
    25261 atgatgcaaa aattccaaaa atgttgttct taatttttca aaatcacctt cataagtatc
    25321 catgtgatca gcatcaatat tagtcactac cgccatcata ggcttcaaaa acaaaaacga
    25381 ggcgtcgctc tcatcagcct ctacaacaaa ataagctgac tttccaagct gagcgttcgc
    25441 accacaacta ttcagctttc cgccaataac aaaactggga tccaaacctc cttcggcaag
    25501 caaactactg accaagctcg ttgttgtcgt tttgccatga gtaccggcaa ttgctatacc
    25561 atgcctaaat cgcattagct cagcaagcat agctgctctg ggaataactg gaatcattaa
    25621 ctcacgagca gccacaattt cgggattatt aaaatcaacg gccgaagacc ttactacaac
    25681 atcagcgcct ttaatgtttt ccagcctgtg accgatatag acttgaatac ctaatgacct
    25741 taagcgttgt accgtgccac tttctccgag atccgaacca gtaatacggt agccctggtt
    25801 atgcaatact tcggcaatac cacacatacc agcaccacca atgccaacaa aatgaatttg
    25861 ctctacacgc cccatgcgcg gagataaaaa ttgctctgag ttattcaata ttccccctta
    25921 agccgttagc ggtttcaacc caagagactg ccaacgattt tcatgatcta tgcgcaataa
    25981 tagtgcaata acgatacaat taattaccat actggcccca ccataactga gtaaaggcag
    26041 agttagccct ttggttggta acagtccggc attaacaccc atattaatag aagcctgtaa
    26101 agctaaccaa atagtcaaac cataggctgt atacgatgcg aaatgccttt cttgagtata
    26161 ggcggtatat cctatgttta atcctctaat aaccagtata ctatacaatg tgatgacaac
    26221 taaaattcca aataacccta attcttcagc aatcacagca aataaaaaat cagtatgtgc
    26281 ctcaggcaaa tagagtaatt tctgtatgct ttcacctaat ccggtaccaa accatccacc
    26341 tcgtccaaaa gcaatcaaag actgagtcaa ttgataacca ctattgtatt gatctgccca
    26401 cggatccaaa aaggctgtta agcgagcaac cctatagggg gaagataccg ccaacagtgc
    26461 aagtgctgtc actacaacca gcattaaacc aaaatagtat cttaatttaa ctccagctaa
    26521 aaataacata gccattactg taccagaaat gacgactgtc gctccaaaat ccggttcgag
    26581 cagtaataaa actgaaacaa cagccagtat tgccatgggt ttaatgaatc caaaaatgct
    26641 ttcacaaacc gcctcttgct gacggaccaa atacccggaa agatagaata tcattgcaag
    26701 tttcgttaat tctgagactt gcacaccaat cggacctaaa gctaaccaac gccgactgcc
    26761 attcactgat ttaccaatac ccgggattaa aacaatgagc aataaaaaca cgcaaccaat
    26821 catcatgggc atgcttattt tttcccagaa actgctgtcc gtacgcacca caattaaagc
    26881 cagcaagagt cccacaaata aataacaagc ctggcgaata agaaaatgaa aaggttgatg
    26941 aaagtacttt gttgaaatca taaccgagct tgatgccacc atcatcaaac caattatcag
    27001 caaaccaaaa actgccccta tcagccattt atcataaagt gatatcggtc tgctaacggg
    27061 tttgcctctt tgatttaaat gtctcggtcg catcgtgttc acaatccgcg cactgaagat
    27121 gtaaacacat cgcccctatg attaaaatca cggaacatat ccaaactggc acaagctgga
    27181 gataacaaaa cgacatcacc aggtttcgca catgtcttgg caatagtcac agcgccctcc
    27241 agagaagaag cacgcacgac aggaactact ttggctaaag ccgattcaat cttgtcagca
    27301 tcttcaccaa tcaaaacaat ggaacgaaca aattctgaaa ctggctgggc taattcttgg
    27361 aaatccgccc cttttccctg tccacccgca ataagaacaa ttttcccttg catagaacct
    27421 cctattccat taatagcaga gattgtagca ccaatatttg ttcctttcga atcattaatc
    27481 caacctactc catccacttc tcttacccac tggcatcgat gtggcagtcc aggaaaggtt
    27541 tttaaaacat taagaatgtg ttgcatagag attcccgctg cttcggctaa agcacaagca
    27601 gcaagcgcat tcatccaatt atgcacccct tttattaaaa tcgattccac aggcaaaaga
    27661 cgctccattc ctttcgccaa ataagtagta ttctcttgtt caatcagccc ccaatttccc
    27721 attgaaggcg cgtctttgcc aaaagagatg cattttatat ctgcttgaca ggattggtga
    27781 ggcacagtgt agacgtcttc acgattaaat aaaacagcct tggcaccact ataaatcctt
    27841 tgttttgctt gcgtataggc ttccatagtg tgatgcctgt ccaaatgatc aggcgttaca
    27901 ttaagaatcg tcgcaaccac tggcgacaaa gaataagtta aatccaattg aaaactggat
    27961 aactccaata cccacaaatc gtagtgatgt tcatcatcca acatatctaa gacaggagtg
    28021 ccgatatttc cggctacagc tacgcgaaac ccggccgcct tagccatttc tcccaccaaa
    28081 gtcgttacag tggatttgcc attagttccc gttatggcaa tgacaggggc gctaatctct
    28141 ctggccaggc attctatgtc tccataaatt gatgcgcccg cttgacgcgc tctctctaaa
    28201 acaggcgtat ccagtgccaa accggggcta gtgataacat ccgtcacctg gctgattact
    28261 tcatcaggag tctgttgcag gtaaattggc acatcaggaa attcattttg gaattcagct
    28321 aatccaggag cctctttacg tgtatcaaaa acaacaaacg atttattatt tctcttcagg
    28381 tatcttgcta tagagagtcc cgtcttaccc agccctgcta ccaaatataa ggaatgattc
    28441 atgaatgcac cttgtctatc gaagttttaa agtagccaat ccgcataaca caaatacaac
    28501 cgtaataatc caaaacctta ctatcacttt gggctctgac caccccttta attcaaaatg
    28561 gtgatgtaat ggggccatcc tgaataatct ttttccgcct gaatatttaa aataacccac
    28621 ttgtaatatg acagaaagcg tctcaattac aaataatcca cccataatga gcagaacaag
    28681 ttcttgccta actacaatcg caactatccc taaagctcct cctaacgcca aagagcccac
    28741 atcgcccatg aaaacttggg caggataact gttataccaa agaaaaccca ggccggcacc
    28801 aacaatagaa gaacaaaaaa tcgttagctc accagtattg ggcacataag ggattcctaa
    28861 ataattagag tatacggcgt tactcgatgc ataggcaaac acacccaagg caccagccac
    28921 cattactatt ggcataatag ccaagccatc cagcccatca gttaaattca ctgcattact
    28981 gcttccaaca ataacgaaat aggcaagtac aggaaaaaaa acgcccagat cccaagtgac
    29041 cgttttaaaa aaaggaactg tgagctgggt atgtactggc aaactggcat tccaatacaa
    29101 ataagagaca gcaactaaag caatgactga ctgccaaaaa tatttccaac gaccaggtaa
    29161 tcctttgcta ttttttaata ccaacttgcg ataatcatca acccaaccta ccaaaccatt
    29221 agccaaggta accaagagca ccagccacag gcttgtttgt cttaaatcgc accaaagcaa
    29281 gcaactcaca gtgatagcca ataaaatcaa aacgcccccc atagtgggag ttcccgcctt
    29341 tgataaatgg gtttggggac catcactcct aaccatttga cctatttgca gccctcttaa
    29401 ccagcggatc attaatgggc cacataacaa acccactata agggcagtta atgaagctaa
    29461 aatagacctg aatgtcaaat attgaaaaac tctaaacgca tgatattgtc cctgcaataa
    29521 ctgcgttaac caatagagca taattattcc tcttgttctt aaaattatgc tcaatgactt
    29581 ttattgagca taattccttt aatggggcaa aaaagtggcc aagcatacca cattcctgtc
    29641 gattcaatga agaggttagc cctctacctg aaccaaccgg tgcacaacat tttccatagc
    29701 actggacctg gatcctttta ccaataccgt ggtatcaggt gttaaatcat tttttaccac
    29761 ttcaatcaac tcttcttgac tgcaaaaatg ctggcctccc tcaccaaacg cctgggatgc
    29821 cagttcacta tgtaaaccgc aactcatcaa cttatctatt cccagctggc gagccgctat
    29881 gccaacttct ctgtgatgtt gtgtcgtcca ttcacccaac tctcccatat caccgaatac
    29941 aaaaattttt cttcctgggc gcccagataa aacttccagg gctgttaaga cagagcgtaa
    30001 attagcattg tatgtatcat caataactgt tgattgattt tgccctgaca acatcgtcat
    30061 ccgacctttt acaccaccaa aatgggccag tcctctttga atgtcattgc ccgatattcc
    30121 tacagcataa caacaagctg cggcagccaa agcatttcgg acattgtgca aacctggtac
    30181 ttgtaactca atctccactc tgctattagg taaaaccaat gtaaaatgag ctcgcccatg
    30241 ggtatccaag cgtatatcct gggcgtaaat atctgcagga tgaagtgagg aaaaacgtaa
    30301 tattttttta tcagcaatca aatcatccca aaagtgagcg taggcatcat catcattgac
    30361 tatagcaaca ccagtcgggg ataagccttg atggatttca cctttggaac gagcgactcc
    30421 gtctattgat ccgaagcctt cgacatgtgc cggggcaata ttattaatta aagacacatc
    30481 aggctgcact atccctaccg tatgcgctat ttctccggga tgattggctc ccaactcaaa
    30541 aacagcatag cggtgctgct tatttaaagc taacatgctt aaaggaaccc ctatgtgatt
    30601 atttaaattg cctttcgtag cgtgtgatgg ctgcggtaaa atagtcgcta tcatctcttt
    30661 gacagtggtt tttccatttg accctgttaa agcaataata gggcaactca tttcctgtcg
    30721 atgtgccgca gcaattttgg ccaaagccat taaaggactg acaaccacaa attgaggaat
    30781 atgaacgccg tcaatcaatc gattaactac agcagctgaa gcgcctttag cctcggcttc
    30841 tttaataaaa tcatgaccat caaagcgttc ccccactatc gcaaaaaata aatttcctgg
    30901 ctttaatgtg cgggtatcta tactaacacc agtaatgtcg gcattaatct ggcaagactg
    30961 agaaagaagt gaagcaattg taattaggtt catagtattt tgtgatgtcg aaaagactag
    31021 tgtactaaca ctcaggctca cagttcaaga tggtttgaga atagatagat aagaatataa
    31081 ctaaaaagtc aagaatttta agtttattac aatccgattc ataagttatc agacattaac
    31141 cattaacaga gtagataatg agcttcgcct ggtgcgaagc cctatcctga aaatataacc
    31201 gctagatagt gcaatgattc tagcctattg gcattagttt gttacacaat catccatcaa
    31261 atgctcaatt ttcaagtatc gatgatatcg tgcgatacgc tcctccgtta gaggtttgcg
    31321 ctggtcatcc agcaaataag catcgagccc ctcacttaat tgttttgcag tatctaacat
    31381 aacagaaaac cgttctgcat tgatctcagg gttatttgat atttgcatga acaggcataa
    31441 tcctcttaca ctgaaagcac cgatattctg taaatcaaaa acaccggttg ctgtagctgc
    31501 agctaaactg caaagcactg gaccttgtcc attcgtgaat tgatgtctat gaaacaagtg
    31561 cctctctcca aatctcaaac cagcagccaa aacagtttgc aataattcat aaccggcaaa
    31621 ttgtcgattt tccttggcca acaaaaacac catggcggtg gaagaagaac agtcactatt
    31681 aggttctgtt ctttcatttg attcgcactc ttcttcctca ggaatcaact gaggttgttc
    31741 tgtttttaca gagggcttcc ttattgcttg tcgcttttca gaaccatcaa aattttcaaa
    31801 atccaaagga acctccctat tgaccttccg cacagcgata atttcatcat tatatgattg
    31861 agagccatag gagttatttt ctacttggcc aagagaaggt tgatagtgtt cttgatttaa
    31921 gctctgtctt ctagtcttca ttaaacggcc aatagccact agaacgccaa ttaacaacag
    31981 gacattaaga attaagctcc aatttgcctg cataagttta ctccatagtc aaagcttgtt
    32041 tcacatcaac cgcaaccaaa cgagacactc ccggctcacg catggtcacc ccaactaaat
    32101 gttcagccaa ttccatagtt accttattat gagtaataaa caggaattgt atgaattgtg
    32161 acatctcttt tactaaatcg caaaaccgac ttacatttaa gtcatctaaa ggcgcatcta
    32221 cttcatctaa catacaaaat ggtgaaggat ttagctgaaa aattgcaaac accaaagcca
    32281 cagcagtcat tgccttttca ccaccagata atagatgaat agaactatta cgttttccag
    32341 gcggctgcgc cattactaca atccctgctt ctaacagatt atcacaagtt aattcgagct
    32401 gtgcccgacc accgccaaat aatcttggaa aaagcgcttt aaaagaggtg ttcacttcat
    32461 caaacgtatt ttcaagacgc gatttcgttt cattatccat tttttcaatt gcagtttcca
    32521 aagtagataa agcttggctc aaatcattat attgctcatc caggaacact ttgcgctgct
    32581 gttcggcagc aaattcttcg atggcagcca ggtttatagc gcctagcctt ttaattttct
    32641 ccaaaatcgc tatcaattcg tcttctttta tactttgcgt gactccggat ggaattcgct
    32701 ctaataaaga ctgggcttgt aaattcgcct catcaagagt ttcttgaatt gaactggcac
    32761 gaacggatag ggcttgtttt tccatttgga tttgattcat gagttcctga atccgtttta
    32821 cttcctgatc atagtttaaa attaatttct cttggtcttc aagttgcaac ttaagctgag
    32881 atactttttc tcggctcaaa gtcaacatcg attcgatttc accatgtttt atcaataatg
    32941 ctgccaattg atttttaaac tcagcttctg gcgctactga ctgtaaataa tgttgggtta
    33001 attcctctaa acgcatctgt gcgcgttgca aacgctcttt ttctctactt atcctatcgg
    33061 ttatttgctg tatcttggtg acttctctat catattccaa ctcactttga tgcaacaaag
    33121 cacggacact ctctacttgt ttattttgtt caacgagaac actgttccat gtgtcttttt
    33181 catttataaa tcgcttgaat tgcaactcag aagcttggta ttgaccttcc agttctctga
    33241 ttttatcagc catttccaat tgttcagcgt ttagctcttc cagcgacgac tccaactcta
    33301 ttatctcgtt cgtgatagca tctttctgtt tttcagcgtg aaaaatagct tgttcctgac
    33361 tatttaccgc aatgatatga ttgcgtagcg cctcattact ggcatttaaa tttaattgat
    33421 gcagtttgat aaaatttaaa ttttcctgta gccgcgcttg ataactatct cttgctaaac
    33481 ggatggattc aattttttgt tgtaaagatt ccacacttaa attcaactcg acaatccttt
    33541 gctgtctggc aagaacacct acctcatctt gctctttttg gcgaggtaat ttaatccacc
    33601 ccttgcctag ccaaatacca ttgacggtta caatagattc atgagccaat aaatcaggaa
    33661 accacgcctt tgcctcttca agattgtctg cggcataaat gtgttcaaga tggtttactg
    33721 ttgagggaac caagccttga attttatcag ccaaccttgg gcgctcctgc ttttccccat
    33781 tgagagctct catcgtcact acgctctctt tatgatcttc gcaaaactgc caccgagacc
    33841 atagctcagc aaaatcatcc aatacataag cgattagatc gtcccctaat acgatttcac
    33901 aagaagattg ccattggggt tcaaccttta aaacatccat taatcttggc ttatctaacc
    33961 attccgcaag tccgtcctta ttagaccgat tttccttttt tgctgcaatt tgagcagctt
    34021 ttaatgcagc aagctcactg tttaaacgat gaaactcatc ttgcaatgca tgcagttgtt
    34081 gctcggtatt ttgtaattga ttctgcaaat ccgattgagt ctcattcgct tgcctaagct
    34141 gttcctcatc aaactcctgt gtttctttca attggaaatg cttttcttgc aatttaattt
    34201 tatttttttg caaatcgtca agcgagatag ctgattgttc caaattcagt ttttccagcc
    34261 ttaccaggac ttgatggcgc ttctgctcta aatgttgtag tttggcttga gtaagttggt
    34321 attcattttt aatggtattg gaattaattt gcacctcttg ccaaattttc tcccattgcg
    34381 attgttcctt ttgtttttct tgccatagag attcctgttc tttgaattcc atttttaaat
    34441 gatgcaaatg ctcctctaac tgttgagcct taagcttgca attgtgcaat tcttcattat
    34501 ccagcttaag ttgcttttca atgagttgag aatcaacctg catttgctgc ctgtcatttt
    34561 ccaaacgctt tttttctctt tcttgttgct gaatggcttc ttcaagacga gctatctcag
    34621 ttcctgattg atagagagaa tcctgaattg attgggattg atcaatggca tcttgtaatt
    34681 gttcatttaa aactgcccgc tccttgtaac aactagttaa taaactttgt tgctgctcat
    34741 tgcggattgc taactcttgt aattctcgct gttttaccgt ttgtttttct gagtattcct
    34801 gccatttaag agcttggatc tcagctcggc aaattttttc ctcttccttt aaaatggtat
    34861 agcgttctgc tgctttggcc tgccgctcca aacgttgcaa atgtttgccc aattcttctc
    34921 taatatcagc gacgcgcgtc aggttatccc ttgtttgttc aatacgttgt aaagtttccc
    34981 ggcgacgctc tttgtatttt gaaactcctg ccgcctcctc caaataggcc cttaattcct
    35041 cgggttttgc ttctattaag cgagatacag taccttggcc aataattgag taacttcgag
    35101 ctcctgctcc tgtgcctaaa aaaatatcag taatgtcttt tcgccggcat cgagctccat
    35161 tcaaataata aaaagattct ccatcccgag tgaccactct cttcacagca atctcaccat
    35221 aactggcaaa aggccctgtc aatcttccca ggctattatc aaataccaat tctactgacg
    35281 cctgccctac cggtttgcga ttggaagagc cgttaaagat aatatccgtc atggactctc
    35341 cacgaagatt tttggcagag ctttcgccca taacccagcg aacagcatca ataatattgg
    35401 atttgccgca tccattcggg ccaacaacag cgaccaaacg actgggaaaa tgaaccgtgg
    35461 tgggatcaac aaatgattta aaaccagcca attttaattg cttcaaatgc attttaactg
    35521 cgaattataa aataataatc ggttagtata tcgtaggaag tgaatcctga ctatttcaga
    35581 acctccatat cgctctcttc tgccatcgtt aaggacaaca tgctcaattg acaaacaatt
    35641 ttatcgcttt ataatgcgta cttctgttaa caaatgtagg taaacaatga aacagcaatt
    35701 ctataaattg ccccaaattc taataagcct ttgtctccta ttgattcttt ccaccccaat
    35761 ccaagcagaa ccgccaaatc ttagtttaat tagaaaagaa attaaaaact attatgactc
    35821 tggcttgtat tatcacgaat tggaacgaac cattaagtta gctcaagaat atatacacca
    35881 acaatattta atcaataaaa acaataaaca cccacaaaaa cttgccatag tactggatat
    35941 tgatgaaacc agcctgtcca actatgataa gatggtaaaa cgggatttta ccggtagcaa
    36001 agagcaaatt cataaagaaa tacttgctgc caattcaccg gcaatcaagc ctatgctgac
    36061 actttacaaa aacgccttaa aacagggtat caaagtattt tttgtaaccg gaaggcaaga
    36121 atccgagcgc gacgcaaccc gcgctaattt aattaaagca ggatatacta aatgggcggg
    36181 actttatcta cggcctaatg ggtattcttc cccctcgatt atcccattta aatcaaaagc
    36241 aagggaaatg atagctaaaa agggatacac catcattgcc agcatcggtg atcaatgcag
    36301 tgatatccaa gggggatatg ctaaaaaagg atttaaactt cccaatccat tttactactt
    36361 gccttagtca aggtgtggtg ggtgattagg tttttcgtag gtcctgttga ttttgaatct
    36421 atgaaacctc aggcacaacg acccgaggtt tcttaatgac tacaagcatt aagctgcttt
    36481 ttctttcata cgaattgcca tgacttccat agccaggaat ttgacataat cataggtcac
    36541 gtaataatct ccttgatctc cagcaaattt ggaccaagaa ttacgcaagg tgaaaacgcc
    36601 tttgttaaca tggccttctt catccatcac ttcaagatca tcatcatagc cagtgatcac
    36661 taattcgtgc ccggcataga tcattccatt cattgcgtcc aatacaattt caggtgttaa
    36721 catccaggtg tcattgtaag cacggttggt acctacagct ccggcatccc caacaaacac
    36781 atccaataac atgccgatag tcaagcggtt acctttcgcc agctcttcct tgatctgata
    36841 gacgatttga ttcatatccg ctttagctga aaaggattcc tcatccttta acaaagcctc
    36901 ccagctaatt aaatttgata ccgggacgct atgcgccagg aactcggagt cagacatggg
    36961 tttcccttca ttgttttcgt cttctaaagg atattctcta acaccggcgc aaccattcaa
    37021 tttctgataa ttctgactga tgataccgta ttcagaaatc tgttgaagaa cccagtaacc
    37081 aaaagaacca ttccacccgc tggctttggc tttgtcatgt atcgccagat aacttcctaa
    37141 ctccagatta cacaattgac taatgtaatc tcctgcccca agggcagcat ctatggctgc
    37201 agttactgcg aaagtgacac aactaccatg atatccctga tccaatacgg gtgttaactg
    37261 cattcccaac ttaacttttc ttggtaactc agaagaaaaa ctggctgtgt ttatactatt
    37321 tttaggatat tctgacagct gttcacgcaa ataagatttc gcttcggtcg ataattcata
    37381 cttgggcaac ttaaatgtta aactttgagc gactccactg ggatttttag gcgtaatgtg
    37441 aacatcaacc tctcctttta aagtcaaagg acctgcaaaa gccagcgatg aagagaaaga
    37501 caatacagct aaattaatta acttactcat aaaatatatc cctataatta accctcttgc
    37561 atcatatcaa gaggtgaatt aaatgcaaac aaatcatgcg aataagaata aaatgacaag
    37621 ctagctttct attcacagag tagaaaccat tttaaaataa ggtacctgtt caccagagtg
    37681 ctgtgataat ttttatcaaa caaattctat cccgaatact gaaataacat cctctcattg
    37741 acgaatcaaa aataacaaat cagattcaat ttaagaaaac aagcaacttg acttgagttc
    37801 atatataata agcctgattc taaaaaagag gatattaata tgcgtactct attgtcatgt
    37861 ttcctgataa ccctattgtc ttttggttta attatcaatg aggcctctgc taaacgcttt
    37921 ggaggcgggc gcagcttcgg ggttcaacgc tctcaaagca gtcttttctc atccaataaa
    37981 acacacaata cgtcttcatt aacacaaaaa gctaataaaa gcagatgggg tggcgtatta
    38041 ggcggcctcc tggttggagg actattggcc agtctcttta tgggcaatgg cttggcaaat
    38101 gggttaatca cctggctaat ccttggttca gtcattttct ttgtcgtcag cttcttacgc
    38161 agaaaaatga atccagggtt tcaagcaact caatccggag cattccgcca aaactctttt
    38221 gaacaattca cgcaaaacta cggaaaccga aatagtggag gagctcattt tgcccaacaa
    38281 gaaaccagct ttgacgcgga caattttctt cgtgatgcca aggtaacttt tatacgtcta
    38341 caagcggctt acgaccaaaa aaatctgcag gatttgcaaa cattcacagt tccggaagta
    38401 tttgcagaaa ttaaaatgca actggacgaa cgaggcgatg cccccaacca aacggaagtc
    38461 atttccctcg atgcaaaatt gcttgatgtg tccaaacaat ccttatccac gatagccagc
    38521 gtacaattca ctggaacaat caaagagaac ggtgaaatat ctaacctgga tgaaatatgg
    38581 catttccgcc agtttgataa cagaaaagag tgggttgtag ggggtattca gcaagaagta
    38641 tttcagccat aaatacttat ggtttcctgg agtggcaact gtctttaatg acagttgcac
    38701 taaaaatcaa agaaaataac tgaaatcact caacaatata ttgcacctgg atgatttcat
    38761 attcaaccat accgccaggc gtttctaccg tgaccgcatc atcaagctcc ttgccaatta
    38821 aagctcgggc aataggagaa ctgtatgaaa tcttgttcag tttgatatca gcttcatcct
    38881 ctccaactat tttataggtt aattctgagc ccgttgctac atgacaaatt gtaacggttg
    38941 aaccaaatat cactttacca ttgttaggca atttactgat atcaattatt tgcgcatgag
    39001 acaacttcgc ttcaagttct tgaatacgcc cttcattaaa actctgttgt tctcgagctg
    39061 cgtgatattc cgcattttct ttcaaatcac catgagctct tgcggttgca attgcttcta
    39121 caatgcgcgg acgatccacg aactttaagc gatgcaattc ttcttttagt gcttctgcac
    39181 cctcaactgt cataggatgt ttactcatat ttacccctaa tgtaaatctt gtaagcgagt
    39241 tacagtttcc ctatcttcat acttcatagc caaacaagca gcttcggctc ctgataaggt
    39301 cgtagtgtag ctgaccttat gctgtaaggc gttgcgtctg atagcaaaag aatctgctat
    39361 cgcttgtttg ccctcagtag tatttacaat aaagtctatt tcattatttt ttataaaatc
    39421 cagcacatgt ggtcggcctt ctgctacttt aaagactctt ctgcagtcta cgccggctgc
    39481 ttgtaatgcc aaagcagtac cgcgcgtggc gataatttca aaacccagtt ctattaatct
    39541 cttggcaatc tcgccgacac gcgttttatc tgcatcacgt acggatacga aagctcgtcg
    39601 ccgtttggca atgtgacatc cggcgcccaa ttgtgcttta gcataagctt gtccaaaacg
    39661 cttggcaata cccatcactt cccccgttga tttcatttcc gggccaagaa tcgaatccac
    39721 accagaaaac ttgataaagg gaaaaaccgg caacttaatc gaatagaaag aaggcgcatg
    39781 atgattttgg tttaatcctt gatctttcaa gctaattcct actttacaaa gagccgctat
    39841 tttagctaat ggcagaccgg tagcctttga aacaaaaggc acggttctcg aagcgcgcgg
    39901 attcacttcc aaaacataaa tatcatccgc ttgaatagca aactgcgcat taatcaagcc
    39961 caccacgcct aatttcaaag ccatttttcg gatctgctct atcaaatcct gctgaacaac
    40021 tacgcccaag ctaaaaggag gtaaagtgca tgccgaatca ccagaatgta tccccgcttg
    40081 ctcgatatgc tccatgatgg caccaattaa tacgtctttg ccatcacaaa cggcatcaat
    40141 atctacctct atggcatcat tcaaaaattt atcaagcaga acgggtgaat cattagaaac
    40201 ttcaactgca tgcgacaggt aataccttaa atcctcttct tgataaacaa tttccatagc
    40261 tcgtccaccg agaacataag aaggcctaac aactagcgga taaccaattt tgttagctaa
    40321 ttcaatggcc tctgcttcgc ttcggacagt tccattatca ggctgatgca gtttcaattc
    40381 tttgaccatt tgctgaaatc gttctctgtc ttcagccatg tcaatagcat ctgatgaagt
    40441 accaattatt tttacgccat tagcctctaa atcacgagcc aactttaatg gtgtttgtcc
    40501 cccataatgc acaatgacac cttccggttg ttccattgcg acgatggaca acacatcctc
    40561 taaagtaaca ggttcaaaat atagtcgatc ggaggtatca aaatcagtgg aaaccgtttc
    40621 tggattgcaa ttcaccataa tagtttggta acccgcactc cttaatgaca aggccgcatg
    40681 cacgcagcaa taatcaaatt caatgccttg gccaattcga ttaggtccac ctccaagaat
    40741 cataatcttt ttcttattgc tctcaggacg tgcttcacag cttccttcat aacaagaata
    40801 catgtaggct gtatcactag ggaattctcc ggcacaagaa tcaattctct tgtagacagg
    40861 caagatctct aattcaacac gtcttgcccg aacttctgct tccgtacaac caagcaattt
    40921 ggccaagtag gcgtcagcaa aaccccggtg tttcatcaag tgtaaagtgg ttttatccag
    40981 atcccgaatc gtttttccaa tgattgagcg ctctaaaaga accaactctt gaatttgtgc
    41041 cagaaaccat ggatcgactc gactttccag atgaatttcc tcaagtgata aattttgcct
    41101 aaaggcatca gcaatatacc aaagtctatc cggagtgggt tctcgtaaat gcccccgcaa
    41161 acgcgccata tcctcttttt gaaacagcga atgcaatcca catcgtccaa tctctaaacc
    41221 tcttatggct ttctgtaaag attcctgaaa gttagaacca atcgccatga cttccccgac
    41281 tgatttcatt tgtgtggtaa gagtgtctgg agtttgcgga aatttatcaa aattaaaccg
    41341 tggaactttg gtaacgacgt aatcaatgct gggctcaaag gacgctggtg ttttacctcc
    41401 ggtgatttcg tttttcaatt catccaaggt ataacccacg gccaattttg ctgcgacctt
    41461 agcaatgggg aaaccggtcg cttttgacgc caaagccgag cttcgagata cacgcgggtt
    41521 catttccaca accagcatgc gcccatcttc aggattaata gcaaactgaa cgttggaacc
    41581 tcccgtatcc acaccaactg ctcttagcac tttaatcgcc gcatcccgca tccgttggta
    41641 ttctttatca gttaatgttt gtgccggagc aacggtaatg gaatctccag tatgcactcc
    41701 catagggtca aaattttcta tggtacaaac aataatgcaa ttatcatttt tatcccggac
    41761 gacttccatt tcatattctt tccaacccag aaccgattca tcaatcaaaa gctcgcgagt
    41821 tggtgatagc tccaatcccc taatgcaaat ttcttcaaat tcttcacggt tataggctat
    41881 accgcctcca ctaccaccca tggtaaatga aggcctgatg atggctggaa agcctaaacg
    41941 agcttgcact tgaattgcct cttccaggct atgagcaatc gctgacctcg gcatatccaa
    42001 tccgatttta atcatcagct ggcgaaattt ttctctatct tccgccctgt ctatggcttc
    42061 acgcgtcgct cctatcattt caacagagta cttggctaat accccttctc ttaccaaatc
    42121 cagagcgcag tttaaggctg tttgtcctcc catcgttggt aaaagagcgt ctggtctctc
    42181 tatctcgata atacgagcca cttctttcca ttgaacaggc tctatatagg ttgcatcagc
    42241 aagctctgga tcagtcatta tggttgcagg attagagtta actaatatca ctcgatatcc
    42301 ttcttctttc aaagcgcgta ctgcttgggt acctgagtaa tcaaactcgc aagcctgccc
    42361 aatcacaata gggcctgctc caagaattag aatagatttt atatcggttc gttttggcat
    42421 aatgacaata aaaaaagaat aaagtgatat attttaccga gttttgccca aagattatac
    42481 ccttcgatga catgttttga gtcatttagt tgcccagaaa acagccaaag gcattaaatg
    42541 acataaaatt acttgtataa attagaaagc agaatttgca aacccgcttc tgtcaaagac
    42601 agatatttat aatagggatc agctgttgtt cgctcaagcc attcatttgc gatgcaaata
    42661 tccgttaatt tctgcgcttc ttccactgaa acaggtttgc ccaatttctg ggtaagcatt
    42721 gctgccgcat cctcttcaat actcaaaaaa acctgctctc tggttacccc gcgcgcgatc
    42781 atttcatcat aaaacaagga caaaatatcc atttcgttca tagcaattcc ttattattag
    42841 atactaagat ctatagcata tatttaatca tcgttcgaca tgataaaatt aaatactttt
    42901 taattaaaaa atttgtcata acctaatctc tctatgcaag aaatcgatgt ccttatcatt
    42961 ggagccgggg ccgcaggcct catgtgtgct attgaggcaa gtaagcgtaa aagaaaagtg
    43021 ttcgtgctgg atcatgccaa taaagccggg aaaaagatac tcatgtcagg aggtggtcgt
    43081 tgtaatttta ccaattacta cattgaaccc aacaagtatt tttcccataa cccccatttt
    43141 ttcaaatcag ccttatctcg ttacactcaa tgggatttta ttgaactggt taacaaacac
    43201 aaaattccct tccacgaaaa aacattagga caattatttt gcgataataa gtccaaagac
    43261 atcgttgata tgttgcttaa agaatgtgag caatatggag tggctattta tttaaatacc
    43321 gtaatagaaa aaatacaaaa aaccaacgac tattctttca aaataggcac gacaaaagga
    43381 aagtttcatt gtcattcttt ggtcattgcg accggaggac tctctattcc cacaatgggt
    43441 gccagcccct ttgcctataa agttgcagaa caattcgcta ttaaagtatg gccaaccaga
    43501 gccggcctgg tgccctttac tttagatgtt ctcgaaaaag acagattatc catcctgtcg
    43561 ggaataggta ttgatagcct ggtcaataat gaaagaaatc aatttcgtga acatatcttg
    43621 tttacccacc ggggactgag tggtcctgct atcttgcaac tctcatccta ctggaatcct
    43681 ggtgaaagta tatgcattaa tttgctgccc gaacataacc tcctggaaag cttgaaaaca
    43741 gcccgagcgg aagccccgca taaacagtta aattctgtat tatcgatgta cttgcccaaa
    43801 cgagttgttg aagtctttat tcctcaaaaa ctgggtgaaa agaaactggc tgacagctct
    43861 aataaagatt tggaaacaat ttcccatttg ttgcagaact gggtagtaaa acccaatggg
    43921 acagaaggct accgtacagc agaagtgaca ataggcggag tagactgtca tgctatttca
    43981 tccaaaacca tggaagccaa caatgtacca ggtctatatt tcattggaga agccctggac
    44041 gtgacggggt ggttaggcgg gtataatttc cagtgggcat ggtcttccgg ctgggctgcc
    44101 ggacaagtag tgtagcgaat ttaaaaacca agtctcaatg cgcatcagct tatctgaatc
    44161 ccaaagccca tatttttccc aggatttcat gaagagcaat ggaagtatag gccaggttat
    44221 aagaattagg tatcaccatt ttgacccagg tatcagtaga ccaaaagaag gtaaaatcag
    44281 ttggggctgc tatggggtgc aatccttgct gctggcataa tgccatggaa cgtggcatgt
    44341 gaatcgctga ggtaaccaga taaaaaggtt gatcatgtac tatggctgct aattcatgcg
    44401 cctgatctgc tgtattaata gaagcagttt caacgacaac tttttccgaa tcgattgcaa
    44461 accacttggc taattctgtc aataataaag cctctgattg ctcaccagca gatcctcccc
    44521 ccgataaaag caattttgcg tcgggcaaag ttctgaataa tcggacgcct tcaatcaatc
    44581 gcttgatgct cgctgaggtc aacagatcat ttgcaggcat ttcctcccca atattttgtc
    44641 ctccaccgag aacgaccacc catttcacat tcggattaac ccgcttcact atagggtatc
    44701 gagactctaa ttgataagtt aaataactgg gtaaccaccc cgtactcata gcaagcaaga
    44761 caagcaatac aacaaacaac ataactttag tgaaagtagg agcaagccga cgatatagta
    44821 aaaaaatgca aatacctaat gtcaaaagac ataaaaagaa aggatgcagc aagtattcaa
    44881 gcaaatgtct aatgaatgcc attatctgac tcttcgcatt aaataaattc caattaaaga
    44941 aaagatgaga gtaggactaa acgcagccaa ttcaggcggc cattggaata ctgtgctcac
    45001 tgggccaaaa aatcgattca gaatatgaaa cccgaatcca accatagcgc ctaccagtaa
    45061 ttttgaaccc atggttgacg aacgtaaggg gccaaaaata aaggggatag ctaatatcat
    45121 cataaccata gtattcaaag gctgggtaat tcgttgaaaa aaggcaaact tataatttgc
    45181 tacattttga tggttgcgtt gttgctcacg taaatagcgg ttcaattcat gtaaagtcat
    45241 ttcatccgga ttagtgcccg taattttaag aatttccggt ttcacaggaa catcccacgg
    45301 caaagtatcg aaatgatagg ctttggtatg atcctctgca aattcagttt gtttcacatc
    45361 ataagctatc cacgaattat tgacatactt tatttcactt atctggcgag ccagttttaa
    45421 attatgctgg ttatcaaagc ggaattgatt tacatagcgc agagtattct cgcgcgtgac
    45481 tgaagcaata gaaacaaaat cattaccatt acggacccaa acccctcttg gagtcagcaa
    45541 agcctgccct cctgttaaag cggaaacctt gtattcattc gcataatgcg ccatgactgg
    45601 tacaacggtt tcacctaaag acgtgattaa caggatgact atcagagacg cctttaatac
    45661 cgcccccgtt atttggccga tggatatccc cgccgcacgc atcacaacca gctcgctatg
    45721 attagctaat actcccaggc caaccaaaca cccgagcaag ctggcaatag ggaaaaacag
    45781 atagacttgg tagggcatct gtaaaaggac aaaaacaccg gcttgtacga ttccatagtc
    45841 tgctcgcccc aaatcgtcta tctgattaac aaacaaaata aagatctgca aaccgaccag
    45901 catcaaagtc accaagccga ttgcagataa aaccgttttg gcaatataac gatcaagtat
    45961 ttttctgctc atgcaagctt cacctgattt cgccaaataa ataggatgcc gagaatggca
    46021 accgccacgt gtacccacca catccctatc caccagggaa tcttcccggt agcaatcgca
    46081 tcacgagaaa caaacataaa attagcataa aaaatataga taatgatggc tggcaaaagt
    46141 ttcgaatatt ttccagaacg agggctgaca cgactaagtg gaacggcaac cagagtcagg
    46201 gtcaaaacca ttaaaggaat ggaaatacgc cactgtaatt cagccgcctt tttcaaatca
    46261 tgattgttca aaggccataa gtctctggtt tttgcagtgc gggcatcatc attcataaca
    46321 acagtcgggt tgggcaattt ggatttatag cttgcaaatt ctgcaatctg gtaatcagca
    46381 tgacccggaa caccttgata ttccttgcca ttttgcagca cgatgtattc ctcgcctgtt
    46441 tgaggattaa cctcagcaaa tgctttatct gcccacaaca catcccataa cagcttatta
    46501 tcctgaacgc tatgttttgc catgaatatc tgttctgctt gggagtgatc acgattcatg
    46561 gattggacat aaaagatttg ttgaccgcta ttaatggcat gaaagcgtcc aggcataatc
    46621 gtttgaatta aagttttgat tcctgtagaa cgtattaact tggtgcgttc aacagcaatc
    46681 acaggacttg cccaaatcat tacgatggca accactacca acacaacagt agccatggca
    46741 aagctgtgtt tcagtaattg cctggggcca taaccacaag ctcgcagaac cgtcatttcg
    46801 ctttcggcat ataaccggcc atacgccaat aaaagagcga cataaaaccc caggggcagt
    46861 aatagaccca ttaaatttgg caactccaac atcaacaact tcatgatcac aaaaccagga
    46921 attgaaccag aagcagcgcg atttaagtat tgtatgaatt gattactcat gaaaataagg
    46981 actaatatcg cggttaaggc gattaaagta agaaacactt ctttagtcaa ataacgaaaa
    47041 ataatcacta caatacccta cagaagacct tatcaattaa ttcaattctt tcagaataac
    47101 atatgaaaga acccatcatg ataattcaag acagcctatt taaaaatagt attgatttaa
    47161 aacagatgat taaaacagaa tttttcattc aaatgcttat ccaatttagg taaactagtt
    47221 gtctttctca agcagtgcag gaaaaaaaat tatgaattat ggattaactc ataccccctc
    47281 attaaccatg agcgaatgtc tggtgttagg cgttttttca gatttagcgc taccagattt
    47341 tgcaactgca attgataatg aacaaaaagg gctgattaaa aaactttgcc agagagtccc
    47401 tgaaccagga aatactgtat ggcaaactga tgttgaagga catagcctat taatcattca
    47461 gtgtggcaaa aaggaagagt ttagcgctaa ttcactccaa aaacgtgtag gagaaattac
    47521 agaagcatta attaaacagc gtttctcttc tgccaccgtg tgcttgcctc ggttaaatca
    47581 ggaatctgct gaatggcagt tagagcaaat gattgttcaa atcgataatt tacgatatca
    47641 acttcttgat tttaaaacga aacatgcaaa aagtcataaa ttggagtcgg taatattcca
    47701 tctgcctgga gctactgaaa aaagccttga aatggccaaa gccattgtta ccggagttga
    47761 attttgccgt gatttagcga atatgccggc caatatttgt actccgactt accttggtga
    47821 gcaagccatt tcattatcca aacaattcga ccaaataagc tgtcaagtga tggggccaga
    47881 agaaataaaa gaaatgggta tgggcgcatt attagccgtt gcccagggaa gtgaccaacc
    47941 cccaagattg atagacattc attatcacgg taacaaaaac tcaacaccgg tcattcttgt
    48001 cgggaaaggc attacttttg actcaggagg cttatctata aagccggcca acgccatgga
    48061 tgaaatgaaa tacgatatgt ctggcgctgc cagtgtgctg ggagttataa aagcctgtgc
    48121 tttgctgaaa ttgccaatta atcttatcgg cattatagcc agtgctgaaa atttgataag
    48181 tggctctgct gtaaaatcag gagacattgt aaccaccatg tcaggacaaa cagtcgaaat
    48241 tatcaatacc gatgcggagg gtcgattagt gctagcagac gctttgactt acgcggagcg
    48301 atataatcct gattttgtaa tcgatattgc tactttaaca ggagcgataa tcgtcgcttt
    48361 gggcaatata gccactggct atatgactag agatgagcag ctggccaaat caatcgaacg
    48421 tgccgctaat gaaagccagg ataaagtctg gcgtatgcca ctggatgagg cctatcaaga
    48481 tgctttggaa agcccattgg ctgatatgat caacgccggc tttgatcgca gtgcaggaag
    48541 tattactgcc gcctgtttct tatcacggtt tactgaaaaa tatcgttggg ctcatttgga
    48601 catcgcggga acagcctgga tttctggaaa gaagcgtaat gcaacagggc gtcctgttcc
    48661 tttattaatt caattattac gccatgtcgc caattcgcgt tgatttttac ttattagcca
    48721 gcagccttcc agaatctcgc tggttggttg cttgtcgttt aatagaaaaa gcctatatca
    48781 aaggacatac tatttttgtt ttttgcgaca atcagcaaca agcagaatac cttgatgaat
    48841 tattgtggac tttcaaagac gacagtttta ttcctcataa tttgcaaggt gaaggaccag
    48901 aacctcctcc tcccgtacaa attggctatc aaaaagaacc cagaggcttt acagatatcc
    48961 tgttgaattt atccaccaat attccaattt tttatacccg atttaaaaga gtcattgaat
    49021 tggtgagtaa tgtagaacat gagaaagaag tgagtagatt gcactataaa gaatataggt
    49081 ataaaggatg tgcattgcac acacactcga ttgaataaat atttttctgc tcgctggatt
    49141 tttaagctta ttgtgtgtcc gctccctgat ggtcgcggct ctgtgttact ttattataat
    49201 cagagccgcg accatcaggg agcggataaa ggataaagaa gcccggagaa tcagcagtta
    49261 catttcaacc tggtgatttt gaataagacc acagcacatc acaatccttt ttttcggcgg
    49321 cttgcaataa gtgaattaaa gggattgctc gtttagccaa actgatatca ggctcactat
    49381 cctcatcgtc ttctagcagt ttattttcgc tttttatttt ttccagccct tgttgtaaat
    49441 gatgcaaagc atcaggtaca ttttcagact taatagcacc ggggattgtg ccgccatgcc
    49501 ccattaacaa caataattgt tttgccacat tttcaaaata agtaatgttc tcatatgcat
    49561 cacaactaaa agtcacaaac ataactagct cccaactgaa aattctatcc gtaagtctgt
    49621 aataaatact cggcaaccgc tcgcaagccc attgcttcac caccttctgg ctttcctggc
    49681 ttacttactg cattccaggc catgatatca aaatgaaccc atggtattga ttttgtcaca
    49741 aatcgttgta gaaataagcc ggcagtaata gccccagcat aggacgaaga actggaattg
    49801 ctcatgtctg caatattcga attcaataac tcttcgtatg ctgaaaataa aggcaaccgc
    49861 cagacggggt ctgagacttt attagatgcc gctactatat cctgagccag ttgatcatga
    49921 ttagaaaaca tggctgcaat ttcagttccg acagcgacac gagcagcacc tgttaaagta
    49981 gcaaaatcaa tgatcaaatc tggtttttct tcacaagcca tagccaaagc atctgccaga
    50041 atcaatcgac cttctgcatc agtgttttct atttctacgg ttaatccgtt gcgcatggtt
    50101 aaaacatctc ctggcctaaa agcattcgga ccaatggcgt tttcaacagc ggggattaat
    50161 acctgtaatc gaatcggcaa acgctgaacc attatccact gggctaaacc tatgacgtgt
    50221 gcggcaccac ccatatcttt tttcatcaaa cgcatgcctg atgaaggttt gatatcaagc
    50281 ccaccactat caaaacaaac accctttcca atcaaagtta cacgtggatg tttctcattt
    50341 ccccaatgca aggataataa acgaggcgtt aaagcagaag ctcttccaac cgcatgaata
    50401 gcaggaaaat tatcctttat caattcctct ccaatccatt gcttaaagca agccttgtgg
    50461 gttttagcca attcttcgac aacatgagct agcgattctg gccccatatc ggaggttggc
    50521 gtgttaatga gatcccgcac gagaaataaa gaattcgcca aggataaaat ataatctcta
    50581 tcatttgcct caacaattaa aactcgaggc tgtaactcgt actttttata ctcatcaaat
    50641 cgatattgtg ctaatgccca aactacgcca gactcacgac ttaacttatc ttgaaattga
    50701 tagttgcctg gaggcaataa aagtgcggca ttggccaaag cactttcctg attaccagtt
    50761 cccgatccaa taaaagcttt ttcaatcacg ccatccgcat tgaggataaa acaataatcg
    50821 cctaatttgc ccttaaactg gcgtacagaa aaacaattgc gttccatggg ggttagattt
    50881 tcaattcctt cgtcccattg cgattgagaa atgagatgga caggaactat cctatcgcca
    50941 taacttttgt aaaatacttc tgcttgcatc tttttaatcc tttatctggc cacggaagtg
    51001 gctaggccta agccctatta tacctaaaac taataaatag atgactacgg tgacgaacac
    51061 atgagctaat aacaagctca aacgcatcaa aggagagaaa ccaagccagt aagtcgctgt
    51121 tccactcata aaatataaat aaactccaat ggcaaaattg gctatcaaca attgcatact
    51181 gtatttcagc catccgggag atggtttgaa caatcccctt ttaatcaaga gaaacaacaa
    51241 aatagcacaa ttcacataac cagctaatgc cgaagccaac gtcaagccag catgagccaa
    51301 gtgccagaca aatacaaaac ataaaagagt attaactacc atggctatgg cacccacttt
    51361 tacaggggtg ctgatgtcct gacgagcata aaaaccggaa gccaacactt taaccaccat
    51421 gaaggcagga actcccatgg caagagtaat caggcttttt tgagtttgta ataaatcata
    51481 ggcagtaaat ttaccataag caaaacaact ggcaatcaga ggcaaggcaa aaaaggacaa
    51541 tcccaaaccg gctggaattc ctattaataa aatcgaacgc agcccccaat ccaaagcacg
    51601 agaatattga ttaatatttt gttctgtgtg ttttctggaa aggtggggca agataaccgt
    51661 cgcaatggcg accccaaaaa ctcccaaagg aaaatcagtt agtctgtctg tatagtataa
    51721 ccaagacaca ctccctactt ttagaaaaga agcaaaaata ctatctacca tcaagttaag
    51781 ttgcgcaatt gaaacaccga ataaggccgg gatcattaac ttaagtactt tattgactcc
    51841 tggatcatcc cgaacaactc tcggcctgat aagcaaatgt cgttgataca aaaatggtaa
    51901 ttgaaaaagc aattgcacaa taccggcaat cagcacaccc catgccaaac caacaacggg
    51961 ttgtggcaaa tcagggcaca aatacaccgc agcaagtatc atactgatat taagcaaaac
    52021 gggagtaaat gctggaaccc cgaaataacc ataggtattt aatattgctc ctgccattgc
    52081 tgttaaggaa ataagcatta aatagggaaa agtgatacga agcatctgag ttgccaattc
    52141 agcccgaacg ctgtcatgat ggaaacctgg cgcgaataaa aaaataataa caggcgatgc
    52201 tacaattccc actacagtga ccagagttaa tatggaactc aaatagcctg aaatccgagc
    52261 gatgaaagta cgaacgtctt cagcagaacg cgttttttga tattcagcca ataccgggac
    52321 aaaagcttga gaaaaagcgc cttccgcaaa aaggcgccgc ataaaatttg gtatcctgaa
    52381 agcaacaaaa aaagcatcca tgccagcttg agctccaaaa aaattggcaa gaaccatatc
    52441 tcgcgcaaac ccgactatcc ttgaaataaa tgtcattaat gaaacaaggg aagtggatcg
    52501 tatcaagctt tgttttttag gtaccataat atccgttgtc agcatgatgt tttaaataga
    52561 taaaaatttt ctttatgata tacttaaact gccgtgagtt cgatataaaa agcatggaaa
    52621 tacctcttat atctggttaa actggcatta tccagtaaaa cacaacacat cagtatacag
    52681 gttccactgc aaatgaagtc gatcatggta tcaaacgagt aagtgaatcg cctgaaatat
    52741 tggttttaca ggatttatcc ggataataaa acatttcaac atcaatctgc gttaaaacaa
    52801 atacataaat acaaaaggtt caaagcaggt ctattgacaa attctaactc attgtgcaag
    52861 atctacatct tttgcaacat ctgattgagt ggagattttt aagtggcaaa tatcaaatca
    52921 gcgattaagc gtgcacgtca aaacgtcaaa ttgcgtcaac ataacgccag cgcgcgttcc
    52981 atgtaccgca cctacattaa aaacgtgctg aaagccgtag agtcaggaga tcaagaagct
    53041 gcgcgtgctg cttataccaa agcacagcca gtgatagaca aagccgctaa taaaggctta
    53101 attcataaaa acaaagcagc acgtattaaa ggccgtctgg ttgcacgtct taaagctatg
    53161 gctgcctaat cgagagtttc cgtcgctcta agttcgaaac taccgaaccg taattcccgg
    53221 ttcggttctc tagtgtagcc cgttagaata attgcttgcc tcgatgagag atttttcatc
    53281 atctgttatc ctgggttctt ggctttgatt ccctttaata aaagttgaat aaaaataatc
    53341 tctacccatt gctgctgcaa ccccctggaa aaagcctaac atacttaatg gttgagcagg
    53401 gaacagcaat gcggctaatt ttctttgtga ctgcatgtgt atcactatat ccaattccga
    53461 ggacatggca tacacatttt ctccggctat tttttgctca ttctctactt tttctatttt
    53521 gccctgtaac tctttttcca aagaacaaaa aggcgccatc atcaatttgg caaattgttc
    53581 tgtaattgct tttaaatgtg agcgattcac ttcaatgctg tcgattgtcg ggttggtaaa
    53641 gccatttgta gtaaagaggc atgtagctac ctcattaagc atgctatgcc aaggtaaaga
    53701 gggtagtaaa aattttgtta gccaatacct gtcgtctttt acagaggaaa aagtggcggc
    53761 caggcactca acaaagaaat ttaatgattc attaaagtac attatccgag catccaattg
    53821 ctccttataa atccctaaac actcatgaca aaaatccgat gccaatttat attgtttttc
    53881 tgctatctgc agacgctccg cataatactc tacagtttca cgaccctttt cgacatcgcg
    53941 ccactcctta ttgatactgc gtgccatgcg ataagccaaa tttaagcctc taaaatgaac
    54001 attagtttgg acttcaaagg ttaactcctc caatgtatgc aattgttcca ttaaggtagt
    54061 aagttggcat aatctcttga ttacttgatt attttctcct gagtggcata cataaataac
    54121 tctgggcact gttcttgagt tgaattgctc ggggaaattt tctgagtgga tcaaagacaa
    54181 ggattgctcc agacgatgct gctgaagttc atcctccgat tgattgacca atattgcatt
    54241 gcggattaga ggaaaacctg tggcttttaa ttgctcctgc attgcttctt ttgtcgaggc
    54301 ttgaaatgca ttgagccaat ttgccttatc ttttgaattg attgcttcct tttgagtatc
    54361 cagccagttt ttggctaaaa catacagtct ctttttagcc aattgaattt ggctttgtaa
    54421 atcatttatg gttaaaaaca tccttttatg ctcacttgct tgcttgagtg tccttaattt
    54481 atcactctaa aatgcatact tcaat
//
LOCUS       DATNSN010000004        53446 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.004, whole genome shotgun sequence.
ACCESSION   DATNSN010000004 DATNSN010000000
VERSION     DATNSN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 53446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53446
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.004"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            145..405
                     /locus_tag="VAH76_000172"
     CDS             145..405
                     /locus_tag="VAH76_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384835.1"
                     /translation="MKILVNTSVLAIVLAFSAPLFADDTQWITEHNLVKGVITFQDDS
                     QIQVYAAQADLDKLQQNLANIQSVEVQPNQTDSTTSPNQQSE"
     gene            complement(447..716)
                     /locus_tag="VAH76_000173"
     CDS             complement(447..716)
                     /locus_tag="VAH76_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEO1384836.1"
                     /translation="MSLGELLLIFIVALIVFGPSKLPMLATHLGMLVRKINQLKEHTS
                     SFWQQQLNEIQLYENQRKAEKADQQYQQTDSENQNSDHKENIRPS"
     gene            complement(713..898)
                     /gene="tatA"
                     /locus_tag="VAH76_000174"
     CDS             complement(713..898)
                     /gene="tatA"
                     /locus_tag="VAH76_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEO1384837.1"
                     /translation="MGLSGISPLSLLLILAIIVALFGTSKLKTIGSDLGEAIKNFRKA
                     MNSEETNDTQKDDHKPL"
     gene            complement(900..2549)
                     /gene="ubiB"
                     /locus_tag="VAH76_000175"
     CDS             complement(900..2549)
                     /gene="ubiB"
                     /locus_tag="VAH76_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEO1384838.1"
                     /translation="MKSIKQLIRLIHINYILAKNGLDNVVVSIKLFAPLRFIVYLNPW
                     NWLRKEKLTRGEALRKSLEELGPIFIKFGQALSTRPDILPEDIAKELSKLQDKVPPFP
                     SHVAMNIIEQAYKQSAYDVFAQFDPIALASASMAQVHAATLKTGENVVVKILRPNMRR
                     LIEQDLNIMYTIAALADRYWPEGKRFKPKEIVKEFEHTLLDELDLMREAANAAQLRRN
                     FNQSPMLYIPEIYWDYCHHNILVIERIHGIPVTDIASLRDHGIDIKKLAERGVEIFFT
                     QVFRDCFFHADMHPGNIFVSYQNPKDPQYICVDFGIIGTLTDNDKRYLAENLVAFFNR
                     DYKRVAELHVESGWVSRDTPVAEFESSIRTVCEPIFEKPLKDISFGQVVFRLFQVARR
                     FHMEVQPQLILLQKTLLAIEGLGRQLYPELDLWATAKPFLEKWVREQMGPKAFIKRLK
                     QNLPFFTEQLPYMPKLIFDILEMKKDQLLTVHELTRLQSENKNQTIQWKSLGLGVFFS
                     FLSLGVISYFDLLDYNHLTTITVAGSVLAGIFVLINRKIRN"
     gene            complement(2639..3256)
                     /locus_tag="VAH76_000176"
     CDS             complement(2639..3256)
                     /locus_tag="VAH76_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEO1384839.1"
                     /translation="MLKEYSLKALQTAINQAMKLDEQMPQKIQKLDGKTLEMVITPLN
                     VNFYIRFKGSEMQLLHRIDGRPDTIIHSNPIGLIRLSLLPTSKARSLFNDKIRISGDI
                     ELGQSVKKLFDEIDIDWEGHLAHFTGDVVAHQIGSFVRKGLEFKNQFSTSMQQNITEF
                     LQEELRICPSRNELEDFFAEVDELVLSVDRLQAHINNLMSDDEGN"
     gene            complement(3264..4016)
                     /gene="ubiE"
                     /locus_tag="VAH76_000177"
     CDS             complement(3264..4016)
                     /gene="ubiE"
                     /locus_tag="VAH76_000177"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEO1384840.1"
                     /translation="MTNQKQTTHFGFKSVDWNEKEKKVAEVFHSVAKNYDRMNDLMSL
                     GIHHLWKRYTIELSHVRPGQSVLDLAGGSGDLTRLLSQKVGDSGQVILADINAAMLHV
                     GRDRLLDEGLFKNIRYVQGNAQCLPFADNSFHCITMGFGLRNVTDKDEALQSMYRVCK
                     PGGKLMVLEFSTPVFPGLKPVYDWYSFNILPKIGKFVANDEASYQYLAESIRMHPDQE
                     TLKAMIERVGFEDCHYHNLSGGIVALHIAYKY"
     gene            complement(4141..4566)
                     /locus_tag="VAH76_000178"
     CDS             complement(4141..4566)
                     /locus_tag="VAH76_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEO1384841.1"
                     /translation="MFYGDTVQETRQLFFISWNKYQNKKELSPLEKEIAQVILDHPEY
                     HKVIEQVQSFPEHTYYPELGETNPFLHMGLHLAVREQVATDRPQGIRTVYTKLIEKYQ
                     DPMAVEHLIMDQLAECLWLSQKNNRPPDENHYLITLQNL"
     gene            complement(4723..5016)
                     /locus_tag="VAH76_000179"
     CDS             complement(4723..5016)
                     /locus_tag="VAH76_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO1384842.1"
                     /translation="MQVKQPAIYIMANKRNGTIYTGVTSDLIKRVYEHKYGDLPGFTQ
                     EHGCKFLVYYELIEDMISAIAREKQLKGGSRKKKLALIEKLNPYWEDLYEKLL"
     gene            complement(5042..5501)
                     /locus_tag="VAH76_000180"
                     /pseudo
     CDS             complement(5042..5501)
                     /locus_tag="VAH76_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212682.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5803..6408)
                     /locus_tag="VAH76_000181"
     CDS             complement(5803..6408)
                     /locus_tag="VAH76_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEO1384843.1"
                     /translation="MLIYFNGLLLGLSLIMALGPQNVFLIRQGARKNHAALSAVICFF
                     CDVILVCGSVAGLHQLLLAHPNLQIWMVWLGSAFLIYYAIKNLKSALSKNKQTVVETH
                     QPHTRMQIILFALGFSLLNPHAIIDTLVIVGSGSSQYPDHKLAFLAGVISASMIWFSS
                     LTMTTRYFSNLLTQNKVWQKIELLSGILMAYIAMKLITSSL"
     gene            complement(6466..7494)
                     /locus_tag="VAH76_000182"
     CDS             complement(6466..7494)
                     /locus_tag="VAH76_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO1384844.1"
                     /translation="MKIFNAMFSKVNGGLEQVFLNYIPALSSQGNLVIPIIHPKAEIK
                     NSCPQDHLITVHNFNQHDFFAIHRLKKLIKIHQPDCIITHSYRAAYLFKKTRTKVPKI
                     AVCHVKGHYDFGTDAIIALTEQMRRDIINSGIEERRVFTIPNLIHIPDDLQYREPKNN
                     QIPTIGVCARFAAIKGVDVFINALAELKKRGVAFQAKIAGDGKEKEQYIELIRHHGLD
                     HEISLLGWIEDKESFYKGIDIFCLPSREESFGLVVLESMMHSLPMVLTRLSGPLEIAG
                     NSGCALFVPPEDPVSMADGLERLLRDKDLSKQLALKAFQRVQHYSSTKVAPMLHKVLE
                     KICDINQQ"
     gene            complement(7560..7913)
                     /locus_tag="VAH76_000183"
     CDS             complement(7560..7913)
                     /locus_tag="VAH76_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEO1384845.1"
                     /translation="MSFKPAVELVKLQQNNREVAVIDGQKILIIWHDNQVHAIQSQCP
                     HLKLPLAKGKITESCSIICPFHKSEFDLKTGEAKCWSPWPPVVGNLLGKISSRKNLKI
                     YPTQIENGQVLVDIG"
     gene            8295..9908
                     /locus_tag="VAH76_000184"
     CDS             8295..9908
                     /locus_tag="VAH76_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEO1384846.1"
                     /translation="MGSLVIKKSIIAALVVALGIAGQSVQAESVPDETLQSEKVQAEK
                     VQAEKTETDLFSPVEQIMNPFYPKYPPTKPATGEQAELIKRGEYLAKMGDCIACHTDV
                     EGGTPAYAGGLPINTPFGLFYSPNITPDKETGIGNWTEKDFIRALKDGRDPKGRNYFP
                     VFPYVYFSKITDDDARALYAYFMSIPPVKLKNKSLPFPFGVPGARFALWGWNLLFFFP
                     QENSIEYDPDKSPEWNRGRYIVDTLGHCSMCHTPMNIFGSAKNRYYLTGSFIDGYWAP
                     NITKYGLESASHQEVADVFKAGELINKAGPVAGPMAEVNHNSLSHLTEADRLAIATYL
                     KTVESEEYLGVSPSDLPPTIARGRQVYLKACLICHQDNKMGAPVIGDSASWYTRLKSS
                     GLTGLYRHAIYGYNQMPVKGACVTCSDNDIISAVDYLLNASLTRSQWQDLASGGVKKY
                     PASGKAVYNENCAVCHNEGKLGAPKIGDKVTWKPLIAQNMDVLIENTIKGKNHLPNGG
                     CNHCSTDEVIEAIKYMVSQSKTEGNYSLW"
     gene            9990..11195
                     /gene="coxB"
                     /locus_tag="VAH76_000185"
     CDS             9990..11195
                     /gene="coxB"
                     /locus_tag="VAH76_000185"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEO1384847.1"
                     /translation="MLNRLNICKLIALFSALTSAQSLWAAADEWQLNMYKGVTPLSND
                     MYYLHMVAMAVCAVIGVVVFGVMIYSLIHHRKSKGYQPASFHDNPKLEIIWTIIPFLI
                     LVGLAVPATRVLIRMDDTQLSDVTVKVVGYQWKWQYQYLDQGISYFSSLSTPYEQIEN
                     KQKKNQWYLLEVDNPLVVPVNKKIRFLVTSNDVVHSWWVPELGVKRDAIPGFMHEAWA
                     RIEKPGVYRGQCAELCGINHGFMPIVVKAVSEEEFDRWVSEQTKVEDIYAEKSDKPRE
                     QKTMTRDQLMTLGKQKYDLICSACHQPNGTGLPPMYPPLKGSSVAVGKPISRHIELLL
                     NGVPGSAMQSYKDQLTDEEIAAIVTYERNAWGNNTNDLIQPADVAALRHGTDTKPTMV
                     NKAKAGGLQ"
     gene            11192..12808
                     /gene="ctaD"
                     /locus_tag="VAH76_000186"
     CDS             11192..12808
                     /gene="ctaD"
                     /locus_tag="VAH76_000186"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEO1384848.1"
                     /translation="MKYAIAHSEDSHEDHGPEQGKGFMGFVKRWLFTTNHKDIGTLYL
                     WLALISFFVAGAMALVIRLELFQPGHRFVDPNFFNQMTTLHGLVMLFGVVMPAFTGMA
                     NWQIPMMIGAPDMALPRLNNWSFWILPFAFCLLFSTMFHAGGGPNFGWTMYAPLSTTY
                     APPSTDFMIFSIHMMGLSSIMGSINIIATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AIMPVLAGAVTMMLADRHFGTSFFNAAGGGDPILFQHVFWFFGHPEVYVLVLPAFGVI
                     SEIIPTFSRKPLFGYHFMVYATVSIAVLSFIVWAHHMFTTGIPLGAELFFMYTTMLIA
                     VPTGIKVFNWVSTMFKGAMTFETPMLFAIAFVFLFTIGGFTGLMLALVPADFQYQDSY
                     FVVAHFHYVLVPGVIFALVAATYYWLPKWTGHMYNERLGKWHFWLSVISVNLTFFPMH
                     FLGLAGMPRRIPDYALQFANFNMIVTVAAFAFGLSQLLFLYNVVATVRKRGTNKDVAT
                     SRVWEGSHGLEWTLPSPPPYHTFTTPPEIK"
     gene            12821..13363
                     /locus_tag="VAH76_000187"
     CDS             12821..13363
                     /locus_tag="VAH76_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEO1384849.1"
                     /translation="MIEEKKQKKLLTILTLLVLGMFAFGFALVPIYNSLCKNLGINGK
                     TKMEAVAYDEDKAKIAADREVLVEFVATNNSGVPWAFYPKTKKIKVHPGEIVKLAFYA
                     ENKTDHTMTVQAIPSVTPGIAAKYLKKTECFCFTQQTLNGHEAMNMPLLFHLDTDLPK
                     NVNTVTLSYTLFDVTGRSSN"
     gene            13522..14391
                     /locus_tag="VAH76_000188"
     CDS             13522..14391
                     /locus_tag="VAH76_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEO1384850.1"
                     /translation="MGAHGTYYIPKPSHWPLVGSIGLTTTLVGAACWLHHDWYGPYVF
                     IAGLCILVAMMFGWFGQVIYENSKGLYDLQVDRSFRWGMCWFIFSEVCFFGAFFGALF
                     FARYWSVPLLGGEMHPITHYTLWPEFKAVWPLLSNPNNQTFAGAHEAMGAWGLAAINT
                     IILLTSGVTITWAHWALKLRKRGQLLTGMSLTIALGVLFLMLQSYEYHEAYTEMNLTL
                     DAGIYGTTFFMLTGFHGLHVTIGTIMLIVILVRCKRGHFTPERHFAFEGVAWYWHFVD
                     VVWLFLFVFVYWL"
     gene            complement(14414..14686)
                     /locus_tag="VAH76_000189"
     CDS             complement(14414..14686)
                     /locus_tag="VAH76_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384851.1"
                     /translation="MLIQPIEPVVITIKIGQNHFECQMISLSESELELKSNDYFEKES
                     QVTFIAKYFRGHAIVRELEFSDYCFTYKMEIVRIQFQPGLLINTRL"
     gene            15267..16337
                     /locus_tag="VAH76_000190"
     CDS             15267..16337
                     /locus_tag="VAH76_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384852.1"
                     /translation="MASSHWNGGVNLQVVEPFFQNNPAFYFLSARRVAAGDANFTVTD
                     IQSQKDVSHDVDIAPEFFLGYVSESGLGFRTRYWFFDQKTRQTAAAPQTSVTDLTLSF
                     PISSAAPIGLDIDTRDNNLRDSMTVETKLNTQIWDGIITKNYEVQNSVIVLGAGARYA
                     YMSQQYNAFETQEPGATLDIRMNPPVPQFITAHKKDLLSGHNFWGWGPVIMIEDTIPL
                     GQSGFSLIGMARGSAVYGPAKQQASKTLLVRGTQNGNEILNENAFVQRKNRHQTVLEI
                     IDAELGVAYSKKWGKGELNGQLSVVAQEWIGGGSASKSATTGAGFGLPGSGMTGAPQV
                     AGVTSESNFGFVGGAFKLGYLY"
     gene            16725..17036
                     /locus_tag="VAH76_000191"
     CDS             16725..17036
                     /locus_tag="VAH76_000191"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1384853.1"
                     /translation="MMMSFAGEGWGEGMSRMVIDDEMWFRLEKLLPKPKGRHGEDDRL
                     FMEAICWMLRTGTAWRDLPLDYGNRKSVYNRYNNWSKKGYITAILTELKKRWRSRMAH
                     A"
     gene            17029..17268
                     /locus_tag="VAH76_000192"
     CDS             17029..17268
                     /locus_tag="VAH76_000192"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1384854.1"
                     /translation="MLDGSIIKVHQDAMRSSYDRSAEAIGSSVGGLSTKIHAKVDSLG
                     QLVNILITPGQVHESQVAHEVWAHESCEFFLADKA"
     gene            complement(17500..17835)
                     /locus_tag="VAH76_000193"
     CDS             complement(17500..17835)
                     /locus_tag="VAH76_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEO1384855.1"
                     /translation="MDKPLQRQRARDLPKNSTNAGRHLWYHLRAGRLGFKFKRQVPIG
                     AYIVDFVYLEKRLVIELDGSQHMDNKIYDTKRTDWLMTNGFKVLRFWNHDVFQQTPSV
                     LEIITSALL"
     gene            complement(17949..18848)
                     /locus_tag="VAH76_000194"
     CDS             complement(17949..18848)
                     /locus_tag="VAH76_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO1384856.1"
                     /translation="MTVKRSSLGRNLSALLSQSSSSLLANEQPQTEQQLKLAVTCLQP
                     GKYQPRGGMEEAPLNELAQSIKKQGVLQPLLVRELDNGKYEIIAGERRWRASQLAGLT
                     EVPVILKQVDDETAMAMALVENLQREDLNAMDQARAMYRLTNEFALTHQQVADLLCKS
                     RTAVSNFLRLLSLSTPVKKLLENGDIDMGHARALLALDEEQQSQVAQLIIAKNLSVRE
                     TEQLVERVKNGKPELKTHHKDNPMFKEQLNSLAKHLQTKIKLKQGKTGKGTLIIHYEN
                     DHNLQKIIEQLLGNFPEVFLDKI"
     gene            complement(18862..19632)
                     /locus_tag="VAH76_000195"
     CDS             complement(18862..19632)
                     /locus_tag="VAH76_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO1384857.1"
                     /translation="MAKVIAIANQKGGVGKTTTAINLSASLAASKQQVLLIDLDPQGN
                     ATMGSGVDKRQLVHTTNDVLLRDCLAEQACLATTCGYDLIPGNEDLTVAEVSLMERNH
                     RETFLFKALQPIQSNYDFILIDCPPALNTLTINAFVAADSVLIPMQCEYYALEGLAAL
                     LSTIEQVKSTVNSRLHIEGVLRTMYDARNRLCAEVSKQLLEHFPAKVYRTVVPRNVRL
                     AEAPSHGMPALQYDKSSPGAAAYMVLAAEVISKQTVAS"
     gene            complement(19635..20261)
                     /gene="rsmG"
                     /locus_tag="VAH76_000196"
     CDS             complement(19635..20261)
                     /gene="rsmG"
                     /locus_tag="VAH76_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO1384858.1"
                     /translation="MIGNTKIKSLLEEGLTAFQLDAIGDLLLDFLLLLNKWNKTYNLT
                     AIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNG
                     KKISFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTEHLVAQ
                     DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE"
     gene            complement(20258..22132)
                     /gene="mnmG"
                     /locus_tag="VAH76_000197"
     CDS             complement(20258..22132)
                     /gene="mnmG"
                     /locus_tag="VAH76_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO1384859.1"
                     /translation="MNLEQLYDVIVVGGGHAGTEAALAAARLGVKTLLLTHNIDLLGQ
                     MSCNPAIGGIGKGHLVKEIDALDGAMAKAADQAGIQFRILNASKGPAVRATRAQADRV
                     LYRKAIRTQLQSQANLTIFQQAVDDLKIEGGLVTGVVTQMGLTLKARAVVLTVGTFLG
                     GKIHVGMNQYAGGRAGDPPSIALSKSLRDLDLPVGRLKTGTPPRIDRRTIDFSQMVEQ
                     PGDTPVPIFSYLGTASDHPQQVPCHITHTTEATHDIIRNNLDKSPMYAGVIEGVGPRY
                     CPSIEDKIVRFADKTSHQIFVEPEGLTTEEIYPNGISTSLPFEVQVQFVRTIKGFENA
                     HITRPGYAIEYDYFDPRGLTSFLQTKAIPNLFFAGQINGTTGYEEAAAQGIIAGMNAA
                     LQIKDQELWCPRRDEAYIGVLIDDLITCGTQEPYRMFTSRAEYRLLLREDNADLRLTE
                     KGRQLGLVGDERWDSFSKKREAIESTQALLHNSWVRVHHNDLFKEALLNPMQHDCRAA
                     EFLKRPEINYQHLLMMDDLNLPELPQEITEQIEIQNKYAGYIDRQQQEIEKLRKHENT
                     MLPETLDYNDVVGLSSEVIQKLNRIKPTSLAQAGRISGVTPAALSLLLVHLKKSRLPV
                     "
     gene            complement(22263..24176)
                     /locus_tag="VAH76_000198"
     CDS             complement(22263..24176)
                     /locus_tag="VAH76_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384860.1"
                     /translation="MATSSLILQSYLKTLKTDEKSELRYEDKVEELLEYNSKAKGGDF
                     LAPLSFYLAILENTDEVMLRYMSEENKQKLIRDLQVAYLLLLTQKKYEEEHQKYENIK
                     TYAQHIKRCEELLDHLNYNQVCKEQKRAPSPEHGYASDGYPVKYLSIFLGKELAEMIV
                     DSMDRKTKTIKESMGWFNEKRLYWVWASSLLKVILAALPDDFFNVSQATDVVKAPDPY
                     TGTLSWALYYFRFSLNMFLLLKHTISGPWMSEREKKTPWTERFLTQWDQRKFTLLNDS
                     IWATGNLICFFWLTGKGALGTWGDVLTLALLVFDISMAIWDYEEQKTRHNKEMLAYEE
                     DIKKLKQLISKAEEEPGKEDEKLRIIKEYELQLQGLMRAQKESERNWDLQKVSLYAGI
                     AYAVGLMLAFALLAAPFFPVSGPALLAITITGAVLCLAFTVIYNGVKGGMEVYKAHCS
                     AKEAKEGYNNKIELFKLLLEKNPSLDDNEKKFLFLEIKKLKAETEYQKQMVVFQTMHL
                     LRSVILESFIPAVVFASFVFLPLGIGFAVLGATIGVALATNLMINAAFKPAKNEVKAF
                     DEKEYEAFCKDPDNWDKPSKSKQGFFQPKEKKPLLDTIAAKTQEKENDRIDLLDDESS
                     VPLLLSSKNTPNI"
     gene            complement(24404..25840)
                     /locus_tag="VAH76_000199"
     CDS             complement(24404..25840)
                     /locus_tag="VAH76_000199"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEO1384861.1"
                     /translation="MTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTM
                     KRAMGLPGNQTIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLAN
                     TVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIE
                     AGTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANI
                     YQFSQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGC
                     WTAVADANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVA
                     DKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKL
                     SLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIE
                     VQSGSYLGEDDIKRFDDIYLRKEVELAE"
     gene            25930..26616
                     /gene="queC"
                     /locus_tag="VAH76_000200"
     CDS             25930..26616
                     /gene="queC"
                     /locus_tag="VAH76_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEO1384862.1"
                     /translation="MKKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELC
                     AATRIAKHMDAADHKIVTLDTALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL
                     AMALGYAESIGARDIFIGASSVDYSHYPDCRPEFIESFQSLANLATKAGIEGDRFTIN
                     APLQYLSKVQTIQLGTELGVDYGLTVSCYQANEAGEACGQCDSCTFRKRGFKSAGVND
                     PTRYQKCVHI"
     gene            26659..27213
                     /locus_tag="VAH76_000201"
     CDS             26659..27213
                     /locus_tag="VAH76_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2885 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384863.1"
                     /translation="MEFFMWDKIKEWYKNNKSYLTMLLIGSAVVGLSVTFLALFFPPV
                     LAAFASISIFGMAPLAFLTSLHLPLAVLTLGCIVTGAAFGAILGGLVAWKQSISIAKH
                     VYSYFGHKQEHQVEINEEDTYRYLMGHLEGEASESFEVEVDEEFNDYTSTDSTEDRPT
                     PLNFSTDSNPQVLDGGYEMTEFRI"
     gene            27523..28260
                     /locus_tag="VAH76_000202"
     CDS             27523..28260
                     /locus_tag="VAH76_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2884 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384864.1"
                     /translation="MTLKQFATGVFYLPAAVFSGLTNLFLGSYAKDKRGNYIQDEDGN
                     YVRNRGLIGLTLDAVKYLGTSVANFISNHQKAISVAFWSSLVVAGAAALTVAFWPAAL
                     AAVVNFSVFGVSIASVVGAGYVAQVAATAGVAAALTSTGVYLGATVVNAFNALRECIS
                     NMRSKKPSSNPEFTPVNDETFEEVRRNQFSGLSKGSEQQQTVQLSSSSSEEEEPVHTP
                     KVFAQPKDVTPEVVENPTSTVSLTGSK"
     gene            complement(28316..28885)
                     /locus_tag="VAH76_000203"
     CDS             complement(28316..28885)
                     /locus_tag="VAH76_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEO1384865.1"
                     /translation="MANNPRLIIGISGSSGIIYGIRLLQVLQKLPIETHLVISKAGQL
                     TRAYETNISAAELKALADVYHPCTDLTASIASGSFKTLGMIIAPCSMKTLGEIAHGIS
                     SNLLTRAADVVLKERRKLVLLPRETPLHLVHLNNMVCITQMGGIICPPVPAFYNHPQN
                     IDDLINDTVGRVLDLFDLDCGLVKRWGET"
     gene            29164..31176
                     /gene="metG"
                     /locus_tag="VAH76_000204"
     CDS             29164..31176
                     /gene="metG"
                     /locus_tag="VAH76_000204"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO1384866.1"
                     /translation="MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQ
                     CISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPEN
                     QALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
                     CGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKL
                     SEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFD
                     EFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKS
                     RGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIAS
                     RCAGFINKRFDNRLSSELINQKLYNDLLSAREFVIDAFVSRDYARAIRQIMDCADKVN
                     QYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFLNSEP
                     LHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPIKENTPVEDSNLI
                     SIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRL
                     TVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPDAGAFPGMKVK"
     gene            31176..31790
                     /locus_tag="VAH76_000205"
     CDS             31176..31790
                     /locus_tag="VAH76_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEO1384867.1"
                     /translation="MASVKEIDALLPQTQCGECGYAGCLPYAEALAQGAAPINKCPPG
                     GVETVNALGKLLHVDASPYLKEAIDNTRPPSVAAIKEDECIGCTKCIKACPVDAIIGS
                     SKLMHAIITHECTGCGLCVDPCPVDCIEMVSLSAAGYDKDLARQRYNAKQMRQLRDEH
                     EKQQIYRERKKLSAHAQDHTQDVQAKQNYILEALARVKAKKTYE"
     gene            31783..32418
                     /gene="nth"
                     /locus_tag="VAH76_000206"
     CDS             31783..32418
                     /gene="nth"
                     /locus_tag="VAH76_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO1384868.1"
                     /translation="MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATD
                     VSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCAILIDQHEG
                     QVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
                     RELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL"
     gene            32659..34410
                     /locus_tag="VAH76_000207"
     CDS             32659..34410
                     /locus_tag="VAH76_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2879 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384869.1"
                     /translation="MLQESIMKWFSSVVNGVKTIGSSLWEGSKWVWNNASLSKITTGV
                     FSYTANTTFQVLEQAIALRDAVPTLVNNPQAKKIAYGMGYILVNDVLPLVTLNFVNNT
                     AQNYFGEGYDEDASWYAPYSLFLSGLALTNYLVKAYTWRQGVQTVAHVAVLDSLGPSS
                     FNSNKKSLPPSICDELDCNYKRKIKGWGREPLVLMGNDALTYGVSLIPYVGEPLSQVL
                     RVYFNGRYIIRLTTPELCERHKYLAMKPESVLGFGLTFEATSMLMDKALESTVGMPPF
                     LYYRALRHLLLLMHINVAAHMELPKSDLKDKASSFDPFNVYERVNRFVADVIFSGLIK
                     RIPIDFKPDKDAEPLIPLSPALKFGTMMLNMDLEKEKKTAPGFFSKAIDKAYVLVLPP
                     VFRGANGLINDPIVFMYWPTIRSGAISAVEFVEGVGKTKTMATLAWAPKTVAAAINLK
                     FGIPKKVTRFVLMLSKEEDFWNLATAFKLWFERHNIKGEPKLITGSNLALNGVTPLQP
                     TPVSVEKEPIVSPHLLITEKDTKEVVPAKSLIPVRSQPQSGVVVTISPDSLFSTRRRR
                     VNPDQEKVAITELNMTK"
     gene            complement(34523..35587)
                     /gene="corA"
                     /locus_tag="VAH76_000208"
     CDS             complement(34523..35587)
                     /gene="corA"
                     /locus_tag="VAH76_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEO1384870.1"
                     /translation="MNTHKTKASAKAGLLPGTAVYIGEHPPQPTQVTIHIYDKKNYKR
                     LDSFDTRQVKEAYEAGHNIWIDISGLADIQKITKICSEYNIHPLVIEDLLNTRQRPKL
                     DIVDDYIFVVFKLLQSPPDHLSYNTEQFCLIIKQKLLLTFRESEKYNLSSIYQRLNGE
                     HSLTREYGSDYLTYLIMDYIVDDYFNFVETSAQLLETLEDQLINKPETIKLQALYTIK
                     RRTITLRKIIPPLRDIVHLLLTDDGGLINKKYNLYFRDLYDHCIRLVESIDLHREMSA
                     SMLDIYLSTINNRMNESMKILTLFASIFIPLSFIAGVYGMNFKFMPELDWRYGYPMIL
                     VTMVLLAILMLYFFKRKKLF"
     gene            35857..37146
                     /locus_tag="VAH76_000209"
     CDS             35857..37146
                     /locus_tag="VAH76_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEO1384871.1"
                     /translation="MNQSLSPQQSPGFLFIAVSMITCLILLINVSFKIVLLGGLVFAI
                     NSLICPLIAALFLFALRNCTFKEQRHLLNICLMTLYMFCIGVYVLVNLPAAEYMHDNP
                     AYQVIFEEIPKKFFATTIAFALSFYLPHLLFCTKNDRVLSTPKQCVLLAVLGGVSFFC
                     LDFYLLFSAPHAHSFKQIFIDSFMISSLLLLIIGVVYLTFLLNDKQHKSKALIETTEL
                     TLFPIYQYLICFAVTVMLVCLACEYRIIAINKDLVLSASCIFFPITIIISSIVGELWG
                     YQANLKLALALLAAQFIFDVLLMGSVALPSPPFFNLNPFYHYIMPRRLSAASLSLFVT
                     FLGNAMLLHYLKYSKWKINRSLRILIANISVNSLLCLVDYSLLYGGIYPYEQIINLAV
                     SVWQYKLLTALIALPVILWMCKILERNYSLVVQTERN"
     gene            complement(37173..38414)
                     /locus_tag="VAH76_000210"
     CDS             complement(37173..38414)
                     /locus_tag="VAH76_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEO1384872.1"
                     /translation="MPSAVIILLIILIAIALRQVVRVVIPIWAIMATGAIAVLLCQQI
                     TPSRAFTAVEPDVMLYLFGVFFISQAAEESGYLAFLTDQIFYYANNGKQTLMLIVFIL
                     GLSSAVLMNDTIAIIGTPIILQLCQSHKNLIKPLLLALAFSITIGSTMSPIGNPQNLL
                     IAIKGEMASPFFEFFKHLAVPTLLNLFIVYFFILFLYKNELKEPIKKPVLPLISDRSN
                     LTLVKMSLLMMLVLTVIKILTDFLHYSEFQLNFSYIALLSSAPIWFSKHRWKYIKRLD
                     WGTLVFFASTFILMQSVWDSGFFQTGMSDSHIDVSRIVVIFIMSIALSQLISNVPLVT
                     IYLPLLMSHNHSSITLLSLAAGSTIAGNLSILGAASNIIIIQNSEKRGIRGFGFLEFI
                     LVGAPLTLLNLLVYISFLQKI"
     gene            complement(38431..39816)
                     /gene="glmU"
                     /locus_tag="VAH76_000211"
     CDS             complement(38431..39816)
                     /gene="glmU"
                     /locus_tag="VAH76_000211"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO1384873.1"
                     /translation="MNLQIIILAAGQGKRMYSDTPKVLHHLAGKPLLTHVVETAQQLN
                     PDAIHVIYGHGGEQIKSSLPNLPVHWVHQAEQLGTGHAVLQAMPHIPDDAYVLVLSAD
                     VPLIQVGTLQSLIECSQRQNPDHSVLALLVAELENPSGLGRIIRNNQGEIYSIVEEKD
                     ANEQVKNIKEIYSGVCCTLANNLKKWLPQLSNSNAQGEYYLTEIISLAVQNKTPITSL
                     TAKNSFEVQGINNRQQLQQLERIWQQRAANQLMEKGATLADANRFDLRGELYCGKDVY
                     IDINCIFTGTVVLGNGCKIGPNCSLTNVTLGDGCEVYANSVLEGCHIANDCHIGPFAR
                     LRSGTQLASHCKIGNFVETKKAIFDEGTKASHLSYLGDVLLGKNVNVGAGTITCNYDG
                     VNKHQTIIEDGVFIGSDTQLVAPVTVGANATIGAGSTIRRNVPPDELTLTESRQKTIY
                     GWKRPVKRERD"
     gene            39938..40822
                     /locus_tag="VAH76_000212"
     CDS             39938..40822
                     /locus_tag="VAH76_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2874 family Dot/Icm T4SS effector"
                     /protein_id="HEO1384874.1"
                     /translation="MNIFLKVLKKLMKEKLSELSANLKWKSETIGYNTKLSIDQKNVI
                     DKLEESIVLFKDSGNDEADLLSINALIDTARIQIQSIRESYGEPRDKGESVTCLNNLK
                     NHSNDFLGKLKKFDFDLLNKAYTETPENIVYYHAAIYLGDEIFSPRTGIDYEIRTKKE
                     VQLAVRLQALSERINPSHNLEEQRKRALQVLQDLAQDNQNAIKKDKGFSLPGLSFWGV
                     SVVTPSDWFSSGEGRFGVEFNTAVRTIQGMTESQFERPTVKQTTAKQEETKPSDVKKT
                     EATDEEDEEHLSENTHTL"
     gene            complement(40920..41930)
                     /gene="ansA"
                     /locus_tag="VAH76_000213"
     CDS             complement(40920..41930)
                     /gene="ansA"
                     /locus_tag="VAH76_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO1384875.1"
                     /translation="MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQE
                     MPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSF
                     MLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
                     QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPRPKAPFHLQTITPHFIANFRLFP
                     GFASDVLEYILNQPLRGLILETYGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQG
                     KVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIPEIRKLIETNLCGEL
                     QI"
     gene            42284..42811
                     /locus_tag="VAH76_000214"
     CDS             42284..42811
                     /locus_tag="VAH76_000214"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEO1384876.1"
                     /translation="MVIDRAGYRLNVGIILVNDSDRVFWGRRSGHDAWQFPQGGLAPG
                     ETAMQAMYRELHEEVGLDKGDVEILGSTRRWLKYRLPKQYLRHGSEPLVIGQKQKWYL
                     LKLVTSEQKVRLDLSDSPEFDSWRWVDFHEPEQQVIFFKRQVYIQALKELEPLLKKER
                     RTPYGLKRKRGNQRA"
     gene            42812..45106
                     /gene="ptsP"
                     /locus_tag="VAH76_000215"
     CDS             42812..45106
                     /gene="ptsP"
                     /locus_tag="VAH76_000215"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO1384877.1"
                     /translation="MLKILKRIVQDVTAASDLKEALGILVQRINTAINAEAVSVYLID
                     NKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLI
                     GEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
                     GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEE
                     LEDEINAFYEALETTRDDIRRLSKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVI
                     REEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRVLAELQRTQREEIVY
                     PKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKV
                     ELVSRRALIVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYR
                     VSLQVNTGLAMDAGLSMSVGAEGVGLYRSEVPFMSRDRFPSEDEQTIIYRQTLKAFAP
                     RLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMRASEELD
                     NLRIMLPMVTTLSEVEEAVYLIEQAFEELVEEGCEIVKPKIGVMIEVPAAAYLAREMA
                     KRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSI
                     CGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRNFAIANARKILAEVLEFEHPA
                     EIRFHLQKALEEEGLGGLIRAGKS"
     gene            45103..45909
                     /locus_tag="VAH76_000216"
     CDS             45103..45909
                     /locus_tag="VAH76_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO1384878.1"
                     /translation="MISGELLINGVIYIIIGAFAGIMAGALGVGGGLIVVPGLVYIFQ
                     HSQVIPEDILMHVAAGCSLAVMIFTSYASLKAHNKMDEILLPLFKKLCPGLVLGTILG
                     SIMASFIPTHLLKIIFGLFLLGVAMKMLLDVRVSHTDKFPGYWLNTLVSSSIGSISGL
                     LGVGGGVIIIPYLTYCGVPVRKIAAVSNLGTFAVGLVGTIMFMITGTLDMTKISYSIG
                     YIYWPAVLGVAIPSSLIAPFGAKLNYLLPVNYLRYGFIVILITTAIHMFL"
     gene            45927..46697
                     /locus_tag="VAH76_000217"
     CDS             45927..46697
                     /locus_tag="VAH76_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO1384879.1"
                     /translation="MLTYPNINPIAFSFGPLKVHWYGLMYLIGFIGAWLLGYWRIKHY
                     KLNWNNDQLSDLIFYSALGVILGGRVGYMLFYDFQEFIHHPWVLFKIWEGGMSFHGGL
                     LGVVIAAWLFCRKYGKTFLEVGDFVAPLVPLGLAAGRLGNFINGELWGRATDVPWGMI
                     YPHVDDQPRHPSQLYEFGLEGVALFILIWCYASKPRQQGRVSALFLMGYAICRLIAES
                     FRQPDSQLGFVAFGWLTMGQVLSIPMLLIGIWLWWAKR"
     gene            46694..47488
                     /gene="thyA"
                     /locus_tag="VAH76_000218"
     CDS             46694..47488
                     /gene="thyA"
                     /locus_tag="VAH76_000218"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO1384880.1"
                     /translation="MKTYLQLLEHILQQGVEKSDRTGTGTLSVFGYQMRFDLTKGFPL
                     VTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWP
                     TADGHTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQI
                     QLTREPLPLPSLTIKRKPASLFDYAYEDFEFVNYQSHPAIKAPIAV"
     gene            47564..47833
                     /locus_tag="VAH76_000219"
     CDS             47564..47833
                     /locus_tag="VAH76_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384881.1"
                     /translation="MKKIVVFVLLAVVCSAGFPSKLSKFLNKMDAEQKQRDAQEWQQD
                     MNFGDYAFRLKERYIDDRGQRCRDYEFRGRSNPYRHGYFTVCDDR"
     gene            complement(47951..48343)
                     /locus_tag="VAH76_000220"
     CDS             complement(47951..48343)
                     /locus_tag="VAH76_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEO1384882.1"
                     /translation="MTEINKIIHKKTFDIAWGDMDALGHVNNARYFDYFQEARIDWLR
                     ELDIKMTGQTGPVVIHVACTFLKPIVYPATVTIHSKAHSLGNSSMIMDHDLYQEETLM
                     AQGVSKIVWIDYTQNKSVPLPDIIRNLV"
     gene            complement(48340..50517)
                     /locus_tag="VAH76_000221"
     CDS             complement(48340..50517)
                     /locus_tag="VAH76_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO1384883.1"
                     /translation="MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGI
                     VINKVCGQQTGHSLKKITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKY
                     RLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
                     NGTQIAALLACQAINLSQQVILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFL
                     LQGVTGSGKTEVYLHVIAKILEQGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSN
                     LNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSA
                     RDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLR
                     SQMVQHGLALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLT
                     LHKQAGQMICHHCGLTQRIPLFCNNCQSKELIPVGSGTQRIYEFLSHQFPDTNVLRID
                     RDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGFYNQDFR
                     AIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQE
                     AEMPPFQFLAVIRAQGKTVNKVAQFLKATKDQIQAHPLKVLGPAPAPLPRKANQHRMQ
                     LLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVDPMDLS"
     gene            complement(50518..51012)
                     /locus_tag="VAH76_000222"
     CDS             complement(50518..51012)
                     /locus_tag="VAH76_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEO1384884.1"
                     /translation="MKKYLTTVELQKESMKFSAGHTTIFSATEREPLHGHMYSVYLAL
                     TTWVEENGMTFDYRYYKERIHKLCRYLNQTFLMPQFSPFLEYSEDEEYYYFTFNKKKI
                     PFLKEDVTLMPLTNITVEELSRWFVNELIKEKEELDRHRIEKVVVKVFSAPGQSASHE
                     WLRS"
     gene            complement(51015..52127)
                     /locus_tag="VAH76_000223"
     CDS             complement(51015..52127)
                     /locus_tag="VAH76_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEO1384885.1"
                     /translation="MNLKQTLINQLKLQKEIPFIDFMQQALYAPYEGYYTSGLQKLGK
                     HGDFITAPELTSLYGKTLANQFQQILPLLDSPVLFEFGAGTGKLCIDTLTHLEQLQCL
                     PESYLILEVSANLRHRQQELIQQKIPQLAGLIHWLDKWPEAPFNGVIIANEVLDAMPV
                     YRFMQSETEILESYVTLDEHDQLAEIFKPVQNQRLLAYIKNHLPPLGYPYLTEANLFL
                     DDWLLNCSHMLKKGALFIIDYGFPRHEYYHPDRNQGTLMCHYQHYSHPNPLLNPGEQD
                     LTAHVDFTHVAEAGHQAGFHIAGYTNQASFLLANGLLSLIHTLDDEQELFAAKQAIKQ
                     LTQPSEMGELFKVIALTKDLEIDLNGFLINDKRVNL"
     gene            52304..53059
                     /locus_tag="VAH76_000224"
     CDS             52304..53059
                     /locus_tag="VAH76_000224"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEO1384886.1"
                     /translation="MNQTNTQEAKVALVTGGAKRIGAAIVKKLHLSGYQVAIHCRSAL
                     HEADILAKDLNRLRTNSAIVMQKELTEPDAAEEIISKVKHWAGRLDLLVNNASVFKRT
                     DCHFLNEADWQYLFAVNVQAPFLLSMAACPLLAPCEGSIINITDIHADKPLKGYSVYC
                     QTKAALEMQTRSLARELAPEIRVNAVAPGAIAWPENDNSLTAEAQQKIIEKTPLKRHG
                     SPDFIAQAVLSLAENTFITGQTLKVDGGRSLTS"
     gene            53116..>53446
                     /locus_tag="VAH76_000225"
     CDS             53116..>53446
                     /locus_tag="VAH76_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384887.1"
                     /translation="MSKWILLSGSFFLCLFSLSVHSHSFDKEQLVQRCQILHDELKEL
                     ESHQYNGVCRHKLALAANKIFSAKIRIVYENYKGAKQDLSVSMNNMKFAEDISCVFKS
                     EITKARME"
BASE COUNT        15272 a        10156 c        11377 g        16641 t
ORIGIN      
        1 tgaaatgttt ttccattatt caatttgttt cttgtccatg tcgtaactcc atgaaaatca
       61 tttaacaaca aaaattgcct ataattataa tgtgtaatat ttttcaactg cataacggat
      121 atcgatttat gcaaggagtg cgttatgaaa atattagtca atacatccgt tttggcaatc
      181 gttttggcgt tttcagcacc actattcgct gatgatacac aatggataac agagcataat
      241 ttggtgaaag gggttatcac ttttcaagat gattcgcaga ttcaggttta tgctgctcaa
      301 gccgatcttg ataaattgca acagaatctt gccaatatcc aatcggttga agtacagcct
      361 aatcaaaccg attctacaac gtcaccaaat caacagagtg aataacacgg gtcttttccg
      421 agttaaacct atgtgctgtt tcgagtttag ctcgggcgta tgttttcttt gtggtcagaa
      481 ttttggtttt ctgaatctgt ctgctgatat tgctgatcag ccttttccgc tttgcgttgg
      541 ttttcataca gttgaatttc attgagttgt tgctgccaaa atgaagaagt atgttctttt
      601 aattgattga tttttcgtac gagcatacca aggtgggttg ccagcatggg aagctttgat
      661 ggcccaaaga caatgagagc aacaatgaaa ataagtaata actcaccaag actcataatg
      721 gtttatgatc gtctttttga gtgtcatttg tttcctcact attcatggct ttgcggaaat
      781 tcttaatcgc ttcgccaagg tcagagccga ttgtttttaa tttggaagtg ccaaacaagg
      841 ctacaataat ggctagaatt aataagagtg ataacggact tattccactt aatcccatgt
      901 tagttcctta tcttgcggtt gatgagtaca aaaattccag ctaaaacaga accagctacc
      961 gtgatggttg tgagatgatt ataatccagt aaatcaaaat agctaatgac acctaatgat
     1021 aaaaatgaga aaaagacacc cagtcccaaa ctcttccatt ggatggtctg atttttattt
     1081 tcagattgca ggcgtgtgag ttcatggact gttaacaact gatccttttt catctccagt
     1141 atatcaaaaa tcagtttagg catatagggg agttgctcag taaaaaaggg cagattttgc
     1201 tttaagcgtt taatgaatgc tttaggcccc atttgttcgc gcacccattt ttcaaggaat
     1261 ggttttgctg tagcccataa atccaattca gggtagagtt gcctgcccaa accttctatc
     1321 gccaacaggg ttttttgcag caaaatcagt tggggctgca cttccatatg aaaccttctg
     1381 gcaacctgga ataaacggaa aactacttgc ccaaaggaaa tgtcttttaa tggtttttca
     1441 aaaataggtt cgcaaacggt tcttatgctg ctttcaaatt ctgcaacagg ggtgtcacgc
     1501 gatacccatc cggattccac atgcaattcg gctacgcgtt tgtagtcacg gttaaaaaaa
     1561 gcaaccaggt tttcagccag ataacgcttg tcattatctg tcagagtccc aatgatgcca
     1621 aaatcaacac aaatatactg agggtctttg ggattttggt aggagacaaa gatatttccc
     1681 gggtgcatgt ctgcatgaaa aaaacaatcc cggaagactt gagtaaagaa aatttctact
     1741 ccacgctcgg ccagtttttt aatgtcaatg ccgtgatctc taagactggc tatatcggtc
     1801 acaggtatgc catgaatgcg ttcaatgacc aggatgttat gatggcaata atcccaatag
     1861 atttcaggta tatataacat cggagattga ttgaaatttc ttcgcaattg agcggcattt
     1921 gccgcttctc tcattaaatc cagttcatcc agtaaggtat gttcaaattc cttgactatc
     1981 tctttgggtt tgaagcgctt tccctcaggc caatagcggt cagccaatgc ggcgatggtg
     2041 tacatgatat ttaaatcttg ttctataagc cggcgcatat tgggtcttag tatttttaca
     2101 acgacatttt cccctgtctt cagagttgcc gcatgcactt gtgccatgga agcggaagcc
     2161 agggcgattg gatcaaattg ggcaaaaaca tcataggcag actgtttata ggcctgttca
     2221 atgatgttca tggcgacatg gctggggaat ggaggaactt tgtcttgcag tttacttaat
     2281 tcttttgcga tgtcttctgg aagtatgtcg gggcgtgtgg aaagggcttg tccgaattta
     2341 atgaagatgg ggccgagttc ctccagcgat ttgcgcagcg cttcaccgcg cgttaatttc
     2401 tctttgcgaa gccagttcca tgggtttaaa taaactataa aacgcaaagg agcaaacagt
     2461 ttgatagaca caacaacatt atcgagacca tttttggcta aaatataatt gatatgtata
     2521 agacgaataa gttgcttaat tgatttcata acagctcatc ggttgttgat ttcgagcttc
     2581 cagccgctca atatgcaatg ataatccatc cagatcctga ataaaattat ccaatcgttt
     2641 aattgccttc atcatcgctc attaaattat tgatatgggc ttgaaggcga tcaacactta
     2701 acaccaattc atcaacctcg gcaaaaaaat cttccagttc atttcttgaa ggacaaatgc
     2761 gtaattcttc ctgaagaaat tcggtgatgt tttgttgcat tgaggtgctg aattggtttt
     2821 taaactccag tcctttacga acaaaagagc caatttgatg agccaccaca tcacctgtga
     2881 agtgagccag gtgcccttcc cagtcaatat ctatttcgtc aaatagtttc tttacacttt
     2941 gccccaattc aatgtcgcct gagatgcgta tcttgtcatt aaataaagag cgggctttcg
     3001 atgtgggtaa taagctcaag cgaatcaatc ctatcggatt gctgtgaatg attgtatcag
     3061 ggcgcccatc aatgcgatgg agcaattgca tttcactccc tttaaagcga atgtaaaaat
     3121 taacatttaa gggggtaatg accatttcaa gtgttttgcc atccagtttt tgaatttttt
     3181 gcggcatctg ctcatcgagc ttcattgcct gattgatggc tgtttgtaat gctttcagcg
     3241 agtattcttt taacataatc aactcaatat ttgtaggcga tatgcagagc gactataccg
     3301 ccacttaaat tatgataatg acaatcctca aatccaaccc gttcaatcat tgcttttaat
     3361 gtttcctgat caggatgcat tctgattgat tctgcaaggt attgatagct ggcttcatcg
     3421 ttagcaacaa attttccgat ttttggcaaa atgttaaatg aataccaatc ataaacgggc
     3481 ttaagtccag gaaaaactgg tgttgaaaat tcaagcacca ttaatttccc acccggtttg
     3541 catactctgt acatggattg cagcgcttcg tctttatcag taacatttct aagcccaaac
     3601 cccatagtga tgcaatgaaa actgttatcc gcaaaaggga ggcattgcgc attaccctga
     3661 acataacgaa tgtttttaaa taatccctca tcaagcaagc ggtcgcggcc aacatggagc
     3721 atggctgcgt tgatgtcggc aagaataact tgtccggagt cgcccacttt ttgcgataat
     3781 aaccgggtta aatcaccact gcccccggcc aaatccaata cgctctgccc aggtcgaaca
     3841 tgacttaatt caatggtata gcgtttccag agatgatgaa ttcccaagga catcagatca
     3901 ttcatgcgat cataattttt agcaacagaa tgaaatactt ctgctacttt cttttctttc
     3961 tcattccagt ctactgattt aaaaccaaaa tgggtggttt gtttctgatt cgtcatattt
     4021 gccggatagt atcattaaag tgttcatatt aaccgataat cattaagata cccagttaaa
     4081 aattgtatac ttttttttat ccatgtacag atgaaaacag gggtatttcc aggatacatg
     4141 ctacaaattt tgcaaggtaa ttaaatagtg gttttcatct ggcggcctat tgtttttttg
     4201 ggaaagccat aagcattctg ccaattgatc catgattaaa tgttcaacag ccatgggatc
     4261 ctgatatttt tctattaact tggtgtaaac agtacggatg ccttgtggcc tatcggtcgc
     4321 tacttgttcg cgaacggcaa gatgaagccc catatgaagg aagggattgg tttcgcctaa
     4381 ttcaggataa taagtatgtt caggaaaaga ttgaacttgt tcaatgactt tatggtattc
     4441 cggatgatca agaatcactt gggcaatttc tttttccaag ggagaaagtt cttttttatt
     4501 ctggtactta ttccagctga taaaaaatag ctgtcgagtt tcttgtactg tatcgccgta
     4561 aaacataatg gcccgattga tataaaatga tccattttaa ctaaataaaa aagtaacaac
     4621 aatgttgatg tgcaaatatg ctctgtacgg tacgattttt gggaccgtcg ttgcgaggag
     4681 cgtagcgacg aagcaatcca gtggccttaa aattgctaat cttcacaaca gtttttcgta
     4741 aagatcttcc caataagggt ttagcttttc aattaaagcc aatttctttt ttcttgaccc
     4801 gcctttaagt tgtttttctc gagctatggc acttatcatg tcttcaatga gctcatagta
     4861 aacaagaaac ttacatccat gctcttgagt aaacccgggt agatccccat atttatgttc
     4921 atagacacgt ttgatcaaat cgcttgtgac acccgtgtag atcgttccat tgcgtttatt
     4981 cgccatgatg taaatggctg gttgtttcac ttgcatgctt cttccttgtt attggaccaa
     5041 ttcattcagc agcgcatatc acgcgactaa tgcacaaaaa cccaagtata tcggagaaag
     5101 aatggtgaat aaactgacag ccattaagcg cagcatcatg taataaatca agtccataac
     5161 gaaaaaaact tactcctttg cgaccatgtt ttttgatttt aattgccttc tgaacatgcc
     5221 gccactctcc agttttatgg gcccaacaaa aagcaatggt caatagcacc agtaattttt
     5281 caatgcgatc cggttgagtc atgtgtgtgt cttcaaaatc gtttgattct tgtgtacctt
     5341 gaatcttgtt tagcagggct agaaaaatca catgcaatct cagttaaatt gaccgttctt
     5401 actttgatta aagagattaa catcgtcgca aaacactcga ttcgcgactt gttccatgaa
     5461 aaataaccat ttaaaatatc tgccagctcg gtgatactca ttcacgactc catgtcgttt
     5521 cttagcggaa aaaatatgga ttatcaatga atcagtgagc tacttcaacg aattcaggcc
     5581 actggattgc ttcgtcgcta cgctcctcgc aacgacggtc ccaaaaatcg taccgtacag
     5641 aggcaaatat ggtaaaaatg ttgacattaa ttagtggagt atgagatatt tattttgcga
     5701 ggttggactt gctatgtgtc attccactgg gtcaattggc gacacactga ttggcccttt
     5761 ctattatcct gaagtcctga caagagctct ctatggctta atctataagc tgcttgtgat
     5821 taatttcatc gcaatataag ccattaaaat accgctaagt aactctattt tttgccatac
     5881 tttattttgg gttaataggt ttgaaaaata acgagtagtc atcgttaatg aactgaacca
     5941 aatcatgctg gcagaaatta cccctgctaa aaaagccagt ttatggtcag gatattgact
     6001 gctgccgcta cccacaatta ccagggtgtc tataatggcg tggggattaa gcagactaaa
     6061 ccccagggca aataaaatga tttgcattct tgtatgaggt tgatgtgttt ctacaacggt
     6121 ttgtttgttt ttggacagcg cgctttttaa gttttttatt gcataataaa ttaaaaaggc
     6181 agagcctagc cataccatcc agatttgcaa gtttggatga gccaaaagca attgatgtaa
     6241 tccagcgaca ctgccgcaca ccaaaatgac atcacaaaaa aagcaaatga cggcagagag
     6301 agcggcatga ttttttcgcg caccttgcct gataagaaag acattttgcg gacctaaggc
     6361 cattatcaat gataatccca agagtaatcc attaaaataa atcaacataa ttgcgtcagg
     6421 ataataaaaa aaaggcgatt atatcataat tttgcgcaag atgttttatt gttgattgat
     6481 gtcgcatatt ttctccagga ctttatgtaa catgggagcg acttttgtgc tggagtaatg
     6541 ttgcactctc tgaaatgctt tgagcgcaag ttgttttgac aggtctttgt ctcgcaagag
     6601 gcgttccaaa ccatccgcca tgctgacagg gtcttctggg ggaacaaaaa gagcacagcc
     6661 tgagttacct gcaatttcaa gtgggccgga taaacgagtg agaaccatgg gcaaggagtg
     6721 catcatgctt tccagtacaa ccaggccaaa agattcttcc cgagaaggca aacagaaaat
     6781 atcaatgcct ttataaaatg actctttatc ctcaatccag ccaagtaaac tgatttcatg
     6841 atccaagcca tgatgacgaa ttaattcgat gtattgttct ttctccttgc cgtcaccagc
     6901 aatttttgct tgaaaagcaa cgcctctctt ttttagttcc gccaaagcgt ttataaagac
     6961 atctacccct ttgattgcag caaatcgggc acaaacacca attgtcggga tctggttgtt
     7021 tttaggttct ctgtattgca gatcgtctgg aatatgaatc aaattgggaa tggtaaatac
     7081 ccggcgctct tctatacccg aattgataat atcccgacgc atttgctctg tgagtgcaat
     7141 aatcgcatcg gtaccaaagt catagtggcc tttgacatgg cacacagcta tcttggggac
     7201 tttggtcctg gtttttttga acaaataggc tgctctgtaa ctatgagtaa tgatgcaatc
     7261 cggttgatgg attttaatta attttttcag gcgatgaatt gcaaaaaaat catgctgatt
     7321 gaaattatgc acagttatta aatggtcttg ggggcaactg ttctttattt ccgctttggg
     7381 atgaataatt ggaatgacca gattgccttg agaacttaaa gctggaatat agtttaaaaa
     7441 tacttgttcc agacctccgt ttaccttgga gaacattgca ttaaatattt tcactttaaa
     7501 tcttcctcat ttagaactgt tcccctattg atatagggag gggttagcaa tgggaatggc
     7561 tagcctatat caaccaatac ttgcccattt tctatctgag ttgggtatat ttttaaattt
     7621 ttccgggagg aaattttacc taaaagattg ccaacaacag ggggccaggg agaccagcat
     7681 ttcgcttcgc cagttttcaa atcaaattcg cttttatgaa aagggcaaat gatagagcag
     7741 gattccgtaa tttttccctt ggccagtggc aattttaaat gagggcactg tgattggatg
     7801 gcatgaactt gattatcatg ccagataatc agtatttttt ggccatcaat cactgcgact
     7861 tctctgttat tttgctgcaa tttgactaat tctacggcag gtttgaaaga cacgttaatt
     7921 acctctatat tgaaaatcag aggattatat cagtgatgaa tttaatctgt tagtagaagg
     7981 ctgataaaat aaatcggttg tgttatcaaa atcccatatt tttgctgagt tagcccaggg
     8041 tatcgtatca atgaccatac ctgaatgatg tctttgagcc tgattatggc atttcatgaa
     8101 atgatgaaat ttcgagaaaa ggttgtgaga tataaggcag gtgacagctg ggttggcata
     8161 attataattt ttaaacaggg ctgaatttaa acggtcgaat aacgttgcaa cttgataagc
     8221 gaggagtata caatgcgaca attattctgt ctcctcatga gagtcgtgaa aaataagaac
     8281 aaataggagg gagcgtgggc tctttggtta taaaaaaatc tattattgcg gcgctagttg
     8341 ttgcactggg tattgcaggg caatctgtac aagctgagtc tgtgccagat gaaacgttac
     8401 aatctgaaaa agtacaggcg gaaaaagttc aggccgaaaa aacagaaaca gatttgtttt
     8461 ctcccgtaga gcaaatcatg aatccttttt atcccaaata ccccccgacc aagccagcta
     8521 ctggcgagca agcagagttg attaagcgtg gtgagtatct ggcaaaaatg ggtgattgta
     8581 ttgcctgcca taccgacgtt gaagggggta cccccgctta tgccggtggt ttacctatta
     8641 atactccttt tggcttattc tatagcccta atattacccc cgataaagaa acgggtatag
     8701 gaaattggac agaaaaagat tttattcgcg ctcttaaaga tggtcgtgat cctaaaggca
     8761 gaaattattt tccagtattc ccatatgtct atttttccaa gataactgac gatgatgctc
     8821 gcgctctgta cgcttatttt atgagcattc ctcctgttaa gttaaagaat aaatcattgc
     8881 catttccttt tggtgtcccc ggggctcgct ttgctttatg gggatggaac ctgttgtttt
     8941 tcttccctca ggaaaattcc attgaatatg atccggataa atctcctgaa tggaatcgtg
     9001 gaaggtatat agtcgatact ttagggcatt gcagtatgtg ccatacaccc atgaatatat
     9061 ttggatcagc aaaaaacaga tattatttga ctggtagttt tatcgacgga tattgggctc
     9121 ctaatatcac caaatatgga ttggaatcag cgtcgcatca agaggtggct gatgtattta
     9181 aggctggtga attaattaat aaagcgggtc ctgtagctgg ccctatggct gaagtgaatc
     9241 ataatagctt gagtcatttg acagaagcag ataggttggc tattgcaact tacctcaaga
     9301 cagtagagag tgaagaatac ctgggtgttt ctccttctga tttgcctccg accattgcac
     9361 gtggaagaca agtgtacttg aaagcctgcc tgatttgtca tcaggacaat aaaatgggtg
     9421 ctccagtgat tggtgattca gccagctggt atacacgtct gaaaagcagc ggtttaactg
     9481 gtctatatcg acatgccatt tatggttata accaaatgcc ggttaagggt gcttgcgtga
     9541 cttgctctga taatgacatt atttcggcag tcgattattt gctcaatgcg tcattgaccc
     9601 gatctcagtg gcaagatttg gcgtctggcg gtgttaaaaa ataccctgcc agtggaaagg
     9661 ctgtatataa tgaaaactgt gctgtttgcc ataatgaggg caaattgggt gctcctaaaa
     9721 taggcgataa agtgacctgg aagccgctga tagcacaaaa tatggatgtt ttgatcgaaa
     9781 acacaatcaa agggaaaaat catttgccaa atggtggttg caatcattgt tcgacagatg
     9841 aagtaattga agcgatcaaa tacatggtca gtcaatctaa aaccgaaggt aattattctt
     9901 tgtggtaggg ttaccagcaa catttgaata agtgaactag cttaaacatt agtcggtttc
     9961 ccggctactt gaagagatga gggattggga tgttaaacag gttaaatatc tgtaaattaa
    10021 ttgcgttatt tagtgccttg acttctgcgc agtcactgtg ggctgctgcg gatgagtggc
    10081 agttaaacat gtacaaaggg gtgactcctt taagcaacga tatgtattac cttcacatgg
    10141 tcgctatggc tgtttgtgcg gtaatcggag tggttgtttt tggcgtcatg atctactcat
    10201 taattcatca cagaaagtca aaaggatatc agccagcaag ttttcatgat aatccaaaat
    10261 tggaaatcat ctggactatt atcccgtttt taatccttgt cgggcttgca gttccagcta
    10321 cccgagtatt aatccgtatg gatgatacgc agctgtctga tgtaacagtc aaggtggtcg
    10381 gctatcagtg gaagtggcag taccagtatc ttgatcaggg tattagttac ttcagtagct
    10441 tatcaacacc ctatgagcaa attgaaaaca agcaaaagaa aaatcagtgg tacttgctgg
    10501 aagtagataa tcctttagtc gtaccagtca acaaaaaaat tcgattttta gtgacatcga
    10561 acgatgtagt ccactcatgg tgggtgcctg agctgggcgt caagcgagac gctatcccgg
    10621 gatttatgca tgaggcatgg gccagaattg aaaagcctgg tgtatacaga ggtcaatgcg
    10681 ccgagctttg cggaattaat catggtttta tgcctatcgt agtgaaagca gttagtgaag
    10741 aagaattcga tcgatgggtt agtgagcaaa ccaaagtaga agatatttat gctgaaaagt
    10801 cggataaacc gcgtgaacaa aaaacaatga cccgtgatca gttaatgacg cttggcaagc
    10861 aaaaatacga tctgatttgc tcagcttgtc atcagcctaa tggcactggc ttgccaccaa
    10921 tgtaccctcc tctcaagggt agttctgttg ccgttggaaa gccaatttca cgacacattg
    10981 aattactcct caatggtgtt cctggctcag caatgcagtc ttataaagat caactgactg
    11041 atgaggaaat agcagccatt gttacgtatg agcgtaatgc ttggggaaat aatactaatg
    11101 atttaattca acccgcagat gttgcggcac tgcgacatgg taccgatact aaacctacca
    11161 tggttaataa agctaaagct ggaggtttgc aatgaaatac gcaatagcgc attcagaaga
    11221 ttctcatgaa gatcatggtc ccgaacaagg caaaggcttt atgggatttg tcaaaaggtg
    11281 gctatttaca accaaccaca aagacatagg tactctgtat ttatggttag cattgataag
    11341 cttctttgtt gcaggagcaa tggctttggt tatcaggctt gagttatttc aacctggcca
    11401 tcgattcgtc gatcctaatt tctttaacca aatgacaaca ttgcatggat tagtcatgct
    11461 gtttggtgtc gtcatgccag cgtttaccgg tatggcaaac tggcaaatcc ccatgatgat
    11521 aggtgctccc gatatggctt tgccaagact aaacaactgg agcttctgga ttttaccatt
    11581 tgctttttgt ctgctgtttt ctaccatgtt tcatgccggt ggcggcccaa attttggctg
    11641 gaccatgtac gcgcctctct ctacaactta tgcaccaccc agtactgatt tcatgatttt
    11701 ttccattcat atgatgggac tctcatccat catgggatca attaatatca tagccacgat
    11761 tttaaacttg agggctccag gcatgaccct gatgaagatg cccctattcg tctggacctg
    11821 gcttattacg gcattccttc tgatcgccat catgcctgtt ctggcgggtg cggttactat
    11881 gatgttggcc gatagacact ttggcacttc tttctttaat gcggcaggtg gaggagatcc
    11941 tatcctgttt caacacgtgt tttggttctt tggtcacccc gaagtgtatg ttctggtctt
    12001 gccagctttt ggggttattt cagaaatcat tcccactttc agccgtaaac cattatttgg
    12061 ttatcatttt atggtttatg ccactgtgag tatcgctgta ttatccttta tagtttgggc
    12121 tcatcatatg ttcaccacgg gaattcccct gggggctgaa ttattcttta tgtacaccac
    12181 catgctgatt gcggtaccta ccggtatcaa ggtgttcaac tgggtgagta caatgttcaa
    12241 aggggctatg acctttgaaa cgccaatgct gtttgccata gcgtttgtat ttttattcac
    12301 tataggtgga tttacaggat taatgttggc attggtaccg gccgattttc aatatcagga
    12361 cagctatttt gttgtggctc acttccatta tgttcttgtg cccggcgtta tctttgctct
    12421 ggtagcagcg acttattatt ggttacccaa gtggacagga catatgtaca atgagcgtct
    12481 gggtaaatgg catttttggc tttctgtcat ttcagttaat ttgacttttt tccccatgca
    12541 ttttctgggg ctagctggta tgccaaggcg aatccctgac tatgctttgc agtttgccaa
    12601 ttttaatatg attgtaacag tggctgcttt tgctttcggt ttgtcgcaat tgttgttcct
    12661 gtacaatgtg gtagccacag taagaaaaag aggcactaat aaagatgtag caacgagcag
    12721 ggtttgggaa gggtcacatg gattggaatg gactttgcca tctccaccac cttaccatac
    12781 gtttactact ccacctgaga ttaaataaag gtggagtttg atgattgaag aaaaaaaaca
    12841 gaaaaaactg ctcaccatat taacattatt agtattaggg atgtttgctt ttggttttgc
    12901 tctggtccct atttataata gcctttgcaa gaatctgggc attaacggca aaaccaaaat
    12961 ggaggctgtt gcttatgatg aagataaggc aaaaattgca gcagatagag aagtcttggt
    13021 tgaattcgta gcaaccaata acagtggtgt gccttgggcc ttctatccca agacaaaaaa
    13081 aattaaagtg catccaggag aaattgtaaa gctggcattc tatgcggaaa ataaaacaga
    13141 ccatacaatg acagttcagg caatcccaag cgtgacacca ggaattgcag caaaatactt
    13201 aaaaaaaacg gaatgttttt gttttactca acaaacacta aatggccatg aggcaatgaa
    13261 tatgccttta ctattccatt tagatacgga tttaccgaaa aatgtgaata cggttactct
    13321 gtcttataca ttgtttgatg tgacaggtag atcaagtaat taggcgtcgt tgatatgtct
    13381 acctttgcgg ctggcttgcc tcgattttct gaacgttgtt tcttgaaatc gtgacaatgc
    13441 aattcacgct agccaaatat caacggtctt tgagtatcag ctttcgctga atgacttatt
    13501 gttacaggag attaaagcat aatgggagca cacggtacat attatattcc caaacccagt
    13561 cactggcctt tggttggctc aataggcttg accactacac tggttggtgc agcatgctgg
    13621 ctacatcatg actggtatgg cccttacgta tttatagcgg gcttatgtat tttggttgca
    13681 atgatgtttg gctggtttgg acaagtcatc tatgaaaaca gcaaaggcct atatgatttg
    13741 caagttgatc gttcatttcg ctggggtatg tgctggttca tattttctga agtatgcttt
    13801 ttcggagctt ttttcggggc cctgtttttt gcaagatatt ggtcagtccc tcttttgggt
    13861 ggtgagatgc atcctattac tcattacacc ttatggcctg agttcaaagc agtttggccc
    13921 ttgctgagta atccaaataa tcagactttt gccggggctc atgaagccat gggggcatgg
    13981 ggtttagctg caataaacac cattatttta ttaacttccg gggtaaccat tacctgggca
    14041 cattgggctc taaaattaag aaagcggggc caattactga ctggtatgtc attgaccatc
    14101 gctttaggcg ttttgttctt gatgttacaa agctatgagt accatgaagc atatacggaa
    14161 atgaatttaa cgctggatgc aggtatttat ggcactacct tttttatgct tacaggattc
    14221 catggtttgc acgtaacaat cggaacgatc atgcttatcg ttattctggt tcgttgcaag
    14281 aggggtcatt ttactcctga acggcatttt gctttcgaag gggttgcgtg gtattggcac
    14341 tttgtggatg tggtgtggtt gttcctattt gtctttgtct attggctata gacttaatct
    14401 aattcatcct atattacaac cgggtgttga taagcaagcc cggttgaaat tgaatacgaa
    14461 caatttccat tttgtaagta aagcagtaat ctgaaaactc taattcacga acgatggcat
    14521 ggcctcgaaa atattttgca ataaaagtaa cctgactttc tttttcaaaa taatcattac
    14581 tttttaattc caattccgat tcagacaagc taatcatttg gcattcaaag tgattttgac
    14641 caatcttaat ggttattaca acaggctcta taggttgaat tagcacagtt tctccttaac
    14701 tgagcggctt gatttgcttc tcttaatagt gacggacatt agattatgaa ttgttcctaa
    14761 tgcgaaaatt cactctacac gattcatgtg taatctaaat gaaataattc tcctcttatt
    14821 tgtattctag accatcaatg atcaaattgt tcatgattta aagatattgt cttgaatagg
    14881 ctcttgttca cgagaggtat tagatggtaa gtgtggcata gcagactatt taatagatta
    14941 taactagaca aacataagac atcattaaaa atgaacctat tcaaaaatta gttgatatca
    15001 aaatacctca gaaacaattt attagattat atttgcctca ttatttgaga tgaagtaaca
    15061 tctctgtcgc tttttccatt tttgataacc tttcgtgatt ttcgccattg ttcgattggt
    15121 gctgtgcaaa tggggatgtg gatgtaaaca atttgacaag gagatatcgg tctatacaat
    15181 ctagtcgttt tatagctgtg acagcaattt atactatcct gctgttatca aagtccacta
    15241 tagctgggac agcaggagat actgccttgg catcaagcca ttggaatggc ggagttaatt
    15301 tgcaagtagt agagccattt tttcaaaata accctgcctt ttatttcctt agcgcaagaa
    15361 gagttgcagc tggtgatgca aattttacag taactgatat ccaatcccaa aaggatgtat
    15421 ctcatgatgt tgatattgct ccagaatttt ttctagggta tgtttcggag tcaggcctcg
    15481 gatttcggac tcgttattgg ttctttgatc aaaagaccag gcaaactgct gcagcccccc
    15541 agacttcggt taccgatttg actttatcct ttcctatttc ttcagctgct ccaatcggtt
    15601 tagatattga tacgcgggat aacaatttac gtgatagtat gactgttgaa acaaaactca
    15661 atactcagat ttgggatggt ataattacca agaattatga agttcaaaac tcagtcatcg
    15721 ttcttggggc aggggcacga tatgcgtata tgtcgcagca atataatgca ttcgaaactc
    15781 aagaaccagg cgcaacatta gatattcgta tgaaccctcc tgtgcctcaa tttataacag
    15841 cacataaaaa agatcttttg tccgggcata acttttgggg ctggggacct gtcattatga
    15901 ttgaggatac aattcctttg gggcaaagtg gtttttcttt gattggcatg gccagaggct
    15961 cagctgttta tggtccagca aagcaacaag ctagtaaaac cttattggta cgaggaacac
    16021 aaaatgggaa tgagatttta aatgaaaatg cttttgtgca aagaaagaat aggcaccaaa
    16081 ctgttttaga aatcatagac gcagaacttg gggttgccta cagtaaaaaa tggggtaaag
    16141 gagagctaaa cggccagctt tcagttgtag cgcaagaatg gattggtggt ggaagtgctt
    16201 caaaatcagc aacaactggc gctggttttg gtttgccagg aagcggcatg actggtgctc
    16261 ctcaagttgc aggagtgaca agtgaaagca actttggttt tgttggaggt gcatttaagc
    16321 ttggatattt atattaattt ttaattgagt ccaaactcat gagagttttc tctttacaat
    16381 actatgcaga aaactctctc acatttttca ctgtaatttt tctaccataa aacattctta
    16441 aataattgga tgactaacca gacaattaat ttgggttgat aaatttgcaa gaactacctt
    16501 taaatttaag aatacctgtc ggggatagga caaaaagcag ccttaacaag gaaggttttt
    16561 ccagttatgt ttcaaaatcc agctattcca taactgtctt taatagattt aatttaagca
    16621 acgtccctga agttttttta gacaagattt aatcttgaat tgagcacgga tttttcaatt
    16681 ttactttgat ctgccccctc tcccgcgcca ggagctttaa tagtatgatg atgtctttcg
    16741 cgggagaggg ttggggagag ggaatgtcac gtatggtcat tgatgacgag atgtggtttc
    16801 gtttagaaaa attacttcca aagcctaaag gccggcatgg tgaagatgat cggctgttta
    16861 tggaggcaat atgttggatg cttcgtactg gtactgcatg gcgtgacttg ccgctggact
    16921 atggcaacag gaaaagtgtt tataatcgtt ataataactg gtcgaaaaaa ggttatatca
    16981 ccgcaatatt gacggaatta aaaaaaagat ggcgatcacg aatggcacat gcttgatggc
    17041 tcgattataa aagtgcatca agatgcaatg agaagcagct acgatagaag tgcagaggca
    17101 attggctcta gcgttggtgg gcttagcaca aaaattcatg ccaaagttga ctcattgggt
    17161 caactcgtca atatactgat aacacctgga caagtacatg agagtcaagt ggctcatgaa
    17221 gtttgggcgc atgagtcttg tgagttcttt ttggcagata aggcttagga tattgatggt
    17281 tttcgaaaag cattaaccga tgatgggata agagctgtta tcccaagcaa aaagaatcgc
    17341 ttgaatccaa cgaaccacga ttgttatatc tacaaagaaa ggaatattgt tgagcgcttt
    17401 ttgcagtaga ttaagcgttt tagacggatt gctacaagat atgataaaac agccaggatg
    17461 tacctcactg gtgtgatgtt tgtaagtata ttattggctt taaagtaacg cagatgtgat
    17521 tatttcgaga acagatgggg tttgctgaaa aacgtcatga ttccaaaagc gcaatacctt
    17581 aaatccattt gtcattagcc aatctgtgcg cttggtatcg taaattttat tgtccatgtg
    17641 ttgactacca tcaagctcga taaccaaccg tttttcaagg tagacaaagt caacgatata
    17701 tgcacctatt ggcacctgtc ttttgaattt aaatccaagt ctacctgctc ggagatgata
    17761 ccaaagatgt cgcccggcat tggtactgtt tttcggtaaa tcgcgagctc gttgtctttg
    17821 caaaggtttg tccatttccc tctccccaac cctctcccgc gaaaggcatc atcatactat
    17881 taaagctcct agtgcgggag aggggggcag atcggagtga aattgaaaaa tccgtgctca
    17941 attcaagatt aaatcttgtc taaaaaaact tcagggaagt tacctaataa ttgctcaata
    18001 attttttgca aattatgatc gttttcataa tgaataatca atgttccttt accggttttc
    18061 ccttgtttta atttgatttt agtctgcaaa tgttttgcta aagagtttaa ctgttcttta
    18121 aacataggat tgtctttgtg atgggttttt aattccggtt ttccgttttt tactcgctca
    18181 accagttgct ccgtttctct gaccgataaa tttttagcaa tgatcaactg agcgacttga
    18241 ctttgctgct cttcatccaa agcaagtaac gctctggcat gacccatgtc tatgtcccca
    18301 ttttccaaaa gcttctttac tggcgttgac agagaaagca aacgaagaaa attgcttact
    18361 gcggttctgg atttgcataa gagatcggct acttgctgat gggtaagagc aaattcattg
    18421 gtgagtctat acatggccct tgcttgatcc atggcattta aatcttcacg ttgcaagttt
    18481 tcaaccagcg ccattgccat ggccgtttca tcatctactt gtttaaggat aacaggaact
    18541 tctgtaagac ctgccagttg gctggcacgc cagcgtcttt ctcctgcgat gatttcatac
    18601 tttccattat ctaactcacg aaccagtaag ggctgtaata cgccttgctt tttaatggat
    18661 tgcgcaagtt cattgagagg ggcttcttcc attcctccac gaggctgata cttgcctggc
    18721 tgcaaacaag tcactgcgag tttgagttgc tgttctgttt ggggttgctc attcgctaat
    18781 aaagagctgc ttgattgact taataaagca gataaattac ggcctaaact actgcgtttt
    18841 actgtcatta cttaccccaa attaactggc tacagtttgt ttacttatca cttcggcagc
    18901 gagaaccata taagctgctg ctcccggtga ggatttatca tattgcaagg caggcattcc
    18961 atgacttggc gcttcggcca gtcttacatt gcgtggcaca acagttctat acacttttgc
    19021 agggaaatgc tctaataact gcttggatac ttcggcgcat aatctgttgc gcgcatcata
    19081 catggtacgc agcacgccct cgatgtgcaa acgcgaattg actgttgatt tcacttgctc
    19141 aatggttgat aacaaagcag ccaatccttc caaagcatag tattcacact gcatgggtat
    19201 caaaacggag tcagcagcaa caaatgcgtt aatggttaat gtattcaaag caggagggca
    19261 gtcaattaaa ataaaatcat aattggactg gatgggttgt aacgccttaa ataagaaagt
    19321 ttcccgatga ttgcgctcca tgaggctaac ttctgcaaca gttaaatctt cattaccggg
    19381 aattaaatca tatccgcaag ttgtagcaag gcatgcctgc tcagccagac agtcgcgtaa
    19441 caagacatcg ttggttgtgt gaaccaactg tcttttatct accccactac ccatcgttgc
    19501 attaccctga ggatctaaat cgatcaacaa aacttgctgt ttggaggcgg caagagatgc
    19561 agataaattg atggctgtgg ttgttttacc tactccacct ttttggttgg caatggctat
    19621 gactttcgcc ataattattc cttgttcgtg ttgttaataa gcacgcagca acgctctcct
    19681 tcgatacccg ggacagcata tctctcaact cgatatgttt gatgaatcgg gaccaattca
    19741 gtatccggaa accgcccttt catggctaac cacaaaccat cctgagcgac aagatgttca
    19801 gtccatttta tcatttgttc gagactgcta aatgctctgc ttattactgt atcaaaacct
    19861 tggtttgggt ggtagttctc tactcgaatt tgtattggtt caatgttctt tatattgagt
    19921 tgacgtttta cttcattgag aaagctaatt tttttcccat tactgtccaa taatacaaat
    19981 tgtaagtcag gtttggcaat agccaaaggg atgccaggta aacccgggcc agtacccaca
    20041 tcgataatat ggtttccctt tatccagggt aaaatagcca gactgtcgaa caggtgcttg
    20101 ctgaccatgg tttcaatatc acgtattgcg gtgaggttat atgttttatt ccatttattg
    20161 agcagaagca aaaaatcaag caagaggtcg ccgatggcat ccagttgaaa cgcagtcaga
    20221 ccctcttcaa gcaatgattt tattttcgta ttacctatca tactggaagc cgactctttt
    20281 tcaggtgaac aagtaaaaga gacagagcgg caggtgttac gccagaaatc cgcccggctt
    20341 gagccaaaga agttggttta atccggttta atttttggat gacttcactc gataaaccaa
    20401 cgacgtcatt ataatccaaa gtttcaggga gcatggtatt ttcatgcttg cgtaatttct
    20461 caatttcttg ttgttgcctg tctatgtacc cggcatattt attctgaatt tcaatttgct
    20521 ctgttatttc ttgcggcaat tcgggtaaat tcaaatcatc catcattaaa agatgctggt
    20581 agttaatttc tggtcgcttc aaaaattcag cggcgcggca atcgtgttgc attgggttaa
    20641 gcaaggcttc tttaaacaga tcattgtgat ggactctcac ccagctgttg tgtaacaaag
    20701 cttgagttga ttcaatcgct tcacgttttt tggaaaagct gtcccatcgt tcatcaccta
    20761 ccaggccaag ttgccggcct ttttcggtca gtcttaaatc ggcattatct tcacgtaata
    20821 acaaacgata ctctgctctt gaagtaaaca tcctataagg ttcttgagtt ccacaagtaa
    20881 tcaaatcatc gataagtaca cctatgtatg cctcatctct gcgaggacac cacaactctt
    20941 gatctttgat ttgtaaggca gcgttcatgc ccgctataat tccctgcgct gccgcttcct
    21001 catatccggt tgtcccattg atttgcccgg caaaaaacaa attaggaatt gctttggttt
    21061 gcaaaaatga ggttaatcca cgtggatcaa aataatcata ctcgatggcg taacctggcc
    21121 tggtgatgtg ggcattttca aaacctttaa tggtgcgcac aaattgcact tgtacctcga
    21181 aggggaggct ggtagaaatt ccattagggt atatttcttc cgttgtcagg ccttccggtt
    21241 ccacaaaaat ctggtgagat gttttatcag caaagcgaac aattttgtct tctatcgagg
    21301 ggcaataacg aggtccaact ccttcaatta ctcccgcata cattggcgat ttatccaaat
    21361 tatttcttat tatgtcatga gtggcttctg tagtatgggt aatatggcat ggcacttgtt
    21421 gaggatggtc gcttgccgtt cccaaatagg aaaatattgg aaccggggta tcacctggtt
    21481 gttccaccat ctggctaaaa tcaatggtgc gtctgtcaat tcgaggagga gttcctgttt
    21541 tcaaacgccc taccggtaaa tccaaatccc gcaagctttt agataacgca atggatggag
    21601 gatctcctgc tctgcctcct gcgtattggt tcataccgac atgaatcttc ccacccagaa
    21661 aagtccctac tgttaataca acagcacgtg ccttgagcgt gagccccatt tgggttacaa
    21721 caccagtgac taatccgcct tcaattttca aatcatcaac ggcttgttga aagatggtta
    21781 aattagcctg tgattgaagt tgtgttcgaa tggcttttct atacaaaaca cgatccgctt
    21841 gtgcgcgtgt ggctcgaacc gcagggcctt ttgaggcatt aagaattcga aattggatcc
    21901 ccgcctgatc ggcggcttta gccatcgctc catcaagggc atcaatttct ttaaccaggt
    21961 gacctttacc aatgccccct atggctggat tgcaggacat ttgcccaagc aagtctatgt
    22021 tatgggttaa taacaaggtt tttacaccca ggcgtgctgc tgcaagcgct gcttctgtac
    22081 cagcatgccc tccgccaacg actatgacat catataattg ttcgagattc atgtttaacc
    22141 attagttatg ccaaaattat aaattggatt tttggatcat ctgcaaaaat gatccaagcc
    22201 aacatataaa aaaatgagca attatacact tttttggcaa ttgtcccaag atgtaataaa
    22261 gattatatat tgggcgtgtt tttgctactt aacaacaggg gtacactgct ctcatcatcc
    22321 agcagatcga ttctgtcgtt ttctttttcc tgtgtttttg cagcgatggt atctaaaaga
    22381 ggttttttct ctttaggctg aaagaaccct tgttttgatt tggagggctt atcccaatta
    22441 tccgggtctt tacaaaatgc ctcatattct ttttcatcaa aggctttgac ttcatttttc
    22501 gcaggtttga aagcagcgtt gatcattaag ttggtcgcca gggcaacacc aattgtggcg
    22561 cccaaaacag caaatccaat tcctaacggt aaaaacacga agctggcaaa aacaacggct
    22621 ggtataaacg attccaaaat caccgaccgc agcaaatgca tggtttgaaa aacaaccatc
    22681 tgcttctggt attcagtttc tgcttttaat tttttgattt ccaggaagag aaattttttt
    22741 tcattatcat ccagcgatgg atttttttcc aataagagtt taaagagctc aatcttgtta
    22801 ttatatcctt ctttcgcttc cttggcggag caatgtgctt tgtagacttc cataccgcct
    22861 ttcacgccat tgtaaatcac tgtaaaagcc aggcaaagaa cagcgcctgt tatggtgatt
    22921 gccagcaaag ccggtccgga aacggggaag aaaggggcag ccagtaatgc aaaagcgagc
    22981 atgagtccga cagcataagc tatgcctgca tataaactga ctttttgcag gtcccaattt
    23041 ctttcagatt ctttttgcgc tcgcattaaa ccttgcaatt gcaattcgta ttctttgatt
    23101 attctgagct tttcatcctc ttttccgggt tcttcttctg ccttgctaat taactgcttt
    23161 aattttttaa tgtcttcctc ataagccagc atttctttgt tgtggcgagt cttttgctct
    23221 tcgtaatccc aaatggccat gctgatgtca aagactaaca gtgccaaagt caatacatca
    23281 ccccaagtcc cgagagcgcc tttgccagta agccagaaaa agcaaatcaa gtttcctgtt
    23341 gcccagatgg aatcattgag caaggtgaat tttctttgat cccattgggt gaggaatcgt
    23401 tctgtccatg gtgtcttttt ttccctctcg ctcatccatg ggccactaat cgtgtgcttg
    23461 agtaacaaaa acatgttgag ggaaaagcgg aaataatata aagcccagct cagggtacct
    23521 gtataggggt caggggcttt gactacatct gttgcctgac tgacattgaa aaaatcatca
    23581 ggtaatgcag cgagtatcac tttgagcagg ctggatgccc acacccaata caaacgcttt
    23641 tcattaaacc agcccatcga ttctttgatg gttttggttt tacgatccat agagtcaacg
    23701 atcatctctg ccagctcttt acccagaaaa atgctcaagt acttgactgg ataaccatca
    23761 ctggcgtaac catgttctgg agagggggct cttttttgtt ctttgcagac ttgattataa
    23821 ttgaggtggt ctagcagttc ttcacatcgt ttaatgtgct gcgcatatgt ttttatgttt
    23881 tcgtatttct gatgttcttc ctcgtatttt ttttgcgtga gtaaaagcaa gtaggcaact
    23941 tgcaagtctc gtatcagttt ctgcttgttt tcttcgctca tgtaacgaag catcacttca
    24001 tcagtgtttt ctaaaatagc cagataaaaa ctcagtggag caagaaaatc gcctcctttg
    24061 gctttgctat tgtattcaag aagttcttct actttgtctt catagcgtag ttcagatttc
    24121 tcatcagttt tgagagtttt caggtaagat tgaagaatta aactactggt tgccataaag
    24181 tcatctcttt tttaattttt gttaattacc tatataaatg atactataaa aagcaaattt
    24241 gttctttttt ataacacagt atttatttta ggtacaagat gaatgcgatt ggctttacaa
    24301 tattgatact aaaatttgca ggaataggga tgaagtgtat tgcaacttag gcaagagggc
    24361 taagcacagt gagtggaatc aagagggtta ttttcgatag acatcattct gccagttcaa
    24421 cttcctttct taaataaata tcatcaaagc gtttgatgtc gtcttcgccc agataggagc
    24481 cactttgtac ttcgataaca taaagtggtt cattatgcgg gttacttaat cgatgcaatg
    24541 cgtttttcgg aatgtaggtg gattggtttt ttgataatcg gaacgtattg tttccattaa
    24601 ctacctcggc ctcaccgcca acgataaccc aatgctcagc ccgatgttga tgcgtttgta
    24661 aagagagttt tgcaccaggc ttcaccataa gccgttttac tttaaatgag gtgccctctg
    24721 ccagcacttc ataatacccc catggtctgg gaacacgttg atgatcttga gttaaatgag
    24781 gatgatcttt gcgtaaggag tgaaccaaat ctttcacttg ttgcgaataa cgtttatcgg
    24841 caactaacac agcgtcactg gttgcaacta tgatttgatc ctgaattcca acggttgtga
    24901 ctaacaactc ttcactatta atgaggcaat tgtgactatc ctgggcgatg acatttccaa
    24961 tgagagtatt gccttgttcg tcttgtttat tcgcgtcggc cacagcggtc cagcagccca
    25021 gatcactcca ttgtattgaa atgggtatta caacagcctt gtctgttttt tccatgatgg
    25081 catagtctat agactcttct ttacattgag aaaaacaatc cagatcaagg cgcaaaaaat
    25141 catgatgatg ctgcgcaagg gttaatgcct gttgactgaa ttggtagatg ttggcttcaa
    25201 attgttctag ctcttcaaga taaacccctg cacgacaaat gaacatgccg ctattccaga
    25261 aataattccc tttactgacg aattgtgcag cagtgtgtgc gtctggtttt tctctaaagc
    25321 tcaaaacttg ctttatatca ctgaataaag tggtgccggc ctcaatgtag ccataaccgg
    25381 ttttggggct atcaggcttt atcccaaatg tcactatcgc attgttttct gaggcaaatt
    25441 gtcctccctc taacatggcg gccagccaga tttcatcatc ggctatccag tgatccgagg
    25501 gtaaaaccag catgacagca tcctttccta ctgtgtttgc caaataatgg gctgcgcatg
    25561 ctatcgcagg ggctgtgttt cttgcacaag gctcaagcag gtaagtggtt tgtgcattaa
    25621 aatcttgcaa ttgatcctga cagataaagt aatgagcatc attacttact ataatggttt
    25681 gattgccagg caatcccatc gctcttttca tggtttgttg aattaaagac aattcgccat
    25741 ttaattttaa aaattgctta ggaaaatttt gtcttgataa aggccatagt cgagtgccag
    25801 agcccccggc taaaattatt ggatatagag gtgtcgtcat cacattccat attgtggcat
    25861 aattgacccg cattttagca ggaattttta agagcaagca attgttccaa ttttttgagg
    25921 ttttgcatta tgaaaaaagc tgtggtcttg ctttctggcg gtttggattc aacgacttgt
    25981 ctcgcgttgg ctaagtctca ggggtttgct tgttatgcat taagcttttc ttacggccag
    26041 aggcattccg cagaactgtg tgccgcaact cgaatagcga aacatatgga tgcggctgat
    26101 cataaaatag tcaccctgga tactgcttta tttggtggct ccgctttgac tgatgcatct
    26161 attgaggttc ccgaattcaa ggaaagcccg gaaataccag tcacctatgt cccagcccgt
    26221 aatactattt ttttggctat ggctttgggt tatgctgaat caattggtgc cagagatatt
    26281 tttattggtg ccagttctgt tgattattct cattatcctg attgtcgccc ggagtttatt
    26341 gaatctttcc aatctttggc aaacttggca acgaaagcag gaattgaagg cgatcgcttt
    26401 actatcaatg ctcctctgca atatctgagc aaagtgcaga ctattcaatt aggtactgaa
    26461 cttggtgttg attacggatt aacggtttcc tgctatcaag cgaatgaagc aggtgaagct
    26521 tgcggacagt gtgatagctg tacttttaga aagcggggtt ttaagagtgc tggagtaaat
    26581 gatcccactc gatatcaaaa atgtgttcat atttaaccca cacagttaaa ttaatgatat
    26641 aatccctgaa attttggttt ggagtttttt atgtgggata aaatcaaaga atggtataaa
    26701 aataacaaga gctatttgac aatgcttttg attggatcgg cggttgtagg tttgagtgtt
    26761 acattcctgg ctctgttctt ccccccagtt ctggcggcat ttgccagcat aagtattttt
    26821 ggaatggcac cattggcttt tttaacgtca ttgcatcttc ctctcgctgt gctaacccta
    26881 ggttgtattg ttactggagc tgcttttgga gcaattttag gaggcctggt ggcatggaaa
    26941 caatcaatta gcattgctaa acatgtttat tcatactttg gtcataagca ggaacatcaa
    27001 gtcgagatca atgaagagga tacctaccgt tatttaatgg gtcatttgga gggagaggcc
    27061 agtgagtctt ttgaagtcga ggtggatgaa gaatttaatg attatacttc tactgattct
    27121 actgaagaca gaccaacccc attgaatttt tcaacagatt caaaccctca ggttcttgat
    27181 gggggctatg aaatgacaga atttagaatc taaaattcga gcacaaatca aatatatctt
    27241 cagggtttac gaaaaacgtt gatcgcttga atttgatcat ttggtttatt caagcatctt
    27301 aattcatggc attttttgct tctatcttgg attttcgaaa atcttgatca cctgatcata
    27361 tattgtaaat tttatcgagc tctcggcaaa aacctggttg agatgaagac aataaaatga
    27421 acttacttta cacttttgta acaacatatt gcgcacaatt aattcacatt taggaaatgg
    27481 gttgctacaa tctgctccga tttataacct tggagtaaaa aaatgacatt aaaacaattt
    27541 gcaactggcg tattttattt gccagcagcc gttttttctg gtttaactaa cctattttta
    27601 ggttcttacg caaaagacaa acgcggtaac tacattcaag atgaagatgg taactatgta
    27661 agaaaccgtg gtttgattgg tttgactctg gacgctgtta aatatttggg aacaagtgtt
    27721 gccaatttca tttctaatca tcaaaaagca atttcagttg cgttttggtc ttctttagtt
    27781 gttgctggtg ctgctgcttt aaccgttgct ttctggcctg ctgccttggc tgctgtagtt
    27841 aacttctctg tttttggagt atcaatcgct tcagtagttg gtgctggtta tgtggctcaa
    27901 gttgctgcta cagccggtgt tgctgctgct ctaaccagta ctggtgtgta tcttggcgct
    27961 actgttgtta atgcttttaa cgcattaaga gagtgcatct ctaatatgcg tagcaaaaag
    28021 ccttccagca acccagagtt tacacctgtt aacgatgaga catttgaaga agtacgtaga
    28081 aaccaattca gtggcttaag caaaggttct gagcaacaac aaactgtaca actttccagc
    28141 tcaagctctg aagaagaaga gcctgtccat actcctaagg tatttgcaca acctaaggat
    28201 gttactcctg aagtggttga gaacccaact tctactgtat ctctcactgg ctcaaagtaa
    28261 tataaaaata ttacaagcaa gtggtcatta aaaacgcggc tttagccgcg tttttttatg
    28321 tttcgcccca gcgtttcacc aatccacaat ccaaatcaaa taaatcgagt actctcccaa
    28381 cggtatcatt gattaagtcg tctatgtttt gcggatgatt ataaaaagcg ggcactggag
    28441 ggcagataat gcctcccatt tgtgtaatac acaccatatt gttcagatga acaaggtgca
    28501 aaggtgtttc cctcggcaag agtaccaatt ttctgcgttc tttaagaacg acatcagcag
    28561 ctcgggtcaa caggttgcta ctgatgccat gcgcaatttc acctaaggtt ttcattgaac
    28621 agggcgcaat gatcataccc aaagttttaa atgagccgct ggcaatggat gcggtgaggt
    28681 cagtgcaggg atgatagaca tctgctaaag ctttgagttc tgcggcacta atatttgttt
    28741 cataagcacg ggtcaattgc cctgctttgc taatgactaa atgcgtttct atgggtagct
    28801 tttgcaagac ttgtaatagt ctaatcccat agattatgcc tgatgagcca ctgatgccta
    28861 tgataagtct tggattgttg gccatgtgta ttcactcgtt gaaaatttaa tttgatcagg
    28921 acttacgacc ccccaatctc tttattaaaa acatttttta ccttgacctt gtggtttctc
    28981 gtagtcctgt tgatattaac aaattataag agaataagta gtacttaaat tatcggatca
    29041 tctcggtaaa agttaacact tcataaataa ctaccaataa aaacctgttt tctattgcag
    29101 catttccatt gtctttgcgt tataattagt cgctttatta taggtgatga gtattctata
    29161 actatgacta gtgaacgaaa aatgttagtg accagtgccc tgccttatgc taatgggcat
    29221 ttgcatctgg gacatttggt tgagcatatt caaacagata tttgggtccg tactcacaaa
    29281 atgttgggta ttcaatgtat tagcgtttgt ggggatgacg ctcatggaac acccattatg
    29341 ctgaaagcag agcagctggg aattactccg gaagctttaa ccgccgaaat taagttaagt
    29401 catgaaaagg attttaaagc cttcgccatt gattatgatt attaccacac cacacattca
    29461 cctgaaaatc aggcattggc gactaccata tttgaacgtt tgcaggcagg cggtgatata
    29521 gtcaagaaaa caattcgcca gttttatgat cccgtaaagc aaatgttttt gccggatagg
    29581 tatgtgaagg gaacttgccc caaatgtgct gctgtcgatc agtatggcga taattgcgag
    29641 gtttgtggag ccacttattc gccgacggat ttgattaacc ctgtttctgt tatatcggga
    29701 gccagcccag tagaaatgga atcagagcat tatttttttg atttgccgcg ttacgaagag
    29761 ttacttaagg attggacaag aaaggggcat ttacaaaccg aagtaacgaa taaactcagc
    29821 gaatggtttg aggcgggttt aaagcaatgg gatatttctc gcgatgctcc ctattttggc
    29881 ttccctatcc ccggtgttcc ggataaatac ttttacgttt ggttagatgc tcccataggc
    29941 tatatggcaa gctttaaaaa gtattgcgat gagcgtggcg tttcttttga tgagttttgg
    30001 gataaggctt caaaaacaga attatatcat tttgtaggca aggatattgt gtattttcat
    30061 gctttgttct ggccagctat gttagctgcc agcggtttca gaacacctac tgccgtgtat
    30121 acccatgggt ttttaacggt tgaggggcag aaaatgtcca aatcgcgcgg tacttttata
    30181 gaagcacgcg cttatctggc tcacttgcat cctgaatatc tgcgttatta tttcgctgcc
    30241 aaattgaatg gtcgagtaga cgatttggat ttgaattttg acgattttgt caatagggtg
    30301 aatgcggatc tggtcgggaa ggtagtgaat attgcaagcc ggtgtgcggg atttattaat
    30361 aagcgctttg ataatcggtt gagctcagag ttgattaatc aaaaattata caacgatttg
    30421 ctgagtgcaa gagaattcgt gattgacgca tttgtttcaa gagactatgc gcgagcaata
    30481 agacaaatca tggattgtgc tgacaaagtc aatcaataca tcgatgccaa taaaccttgg
    30541 gtattggcta aagatgaaag taaactaaat gaagttcatg ccatatgcac aatgggaatc
    30601 aatttattcc gcattttaat aacctattta aaaccagtct tgccaatgat ggccaaagct
    30661 tcagaagaat ttttaaattc tgagccttta cactggggtt ctattgataa gcctttgcta
    30721 aaccatcgta tcaatacttt taagcctttg atggtcagag tagaaaaaga aaaaattgaa
    30781 gcgatgttgg ttcaaagcaa ggaatctctg atgactaccc caataaaaga aaacacgcca
    30841 gttgaggact ctaacttaat cagtattgag gattttgcca aagtcgattt acggattgca
    30901 aaaattgtca atgcagaacc tgtggaaggc gcagataaac taatgcgctt gattttagac
    30961 ttgggtgatg ctcaaaagca ggtttttgcc ggcataaaaa aggcttatga tgctgaagag
    31021 ttgattgggc gtttgactgt aatggttgct aatttggagc cccgcactat gcgtttcggt
    31081 gtttctgaag gcatggtatt ggcggcaggg gatggacagg gaatttattt attacagcct
    31141 gatgctggag catttcctgg catgaaggtg aagtaatggc ctctgttaaa gaaattgatg
    31201 ccctattgcc gcaaacccag tgtggggaat gcggatatgc aggttgtttg ccttatgctg
    31261 aagcgcttgc ccaaggagct gcccctatta ataaatgccc tcctggtggt gttgaaactg
    31321 taaatgcttt gggaaaatta ctgcatgttg atgcatctcc atatttgaaa gaagcaatag
    31381 ataatactcg gcccccttca gttgctgcta tcaaagagga tgaatgcatt ggttgtacta
    31441 aatgcatcaa ggcctgtcct gtggatgcaa ttattggtag tagtaaatta atgcatgcca
    31501 ttattactca tgagtgcact ggatgcggct tgtgtgtgga tccttgtcct gttgactgca
    31561 ttgagatggt tagtttgtct gcggctggat atgataagga tttggcccgc cagcgttata
    31621 atgccaaaca aatgcgccag ttgcgggatg agcatgaaaa acaacaaata tacagagaaa
    31681 gaaagaaact ttcagcccat gcgcaagatc atactcaaga cgtacaggca aaacaaaatt
    31741 atattcttga ggctttggct cgggtgaaag caaaaaagac atatgaataa acaaaaatca
    31801 caagaaattt tcaaacgttt tcaagaacaa aaccctcatc ctacaacgga gttgcaatat
    31861 agtaccccct ttgagttatt aattgccgtc atgctttcag cccaggcaac agacgttagt
    31921 gtgaacaaag cgacagccaa actattcccg gtcgccaata ccccacaagc cttgctggat
    31981 ttgggattag atagattaaa agattatatt aagtccatag gtctttataa tagtaaagca
    32041 caaaatatca tgaaaacctg tgcaatactt attgatcagc atgagggaca agttcctggc
    32101 cagagagagg cgctggaagc attgcctggt gttggccgaa aaactgcaaa tgtggttttg
    32161 aatacagcat ttggtcagcc aaccatagcg gtcgacaccc atattttcag agtggctaac
    32221 cggattggca tagcgaaggg taatacaccg ctggcggttg agcgggagtt attaaaaaaa
    32281 gtgccccgtg agtttttaca tgacgctcat cactggttaa tattacatgg ccgttatgtc
    32341 tgtacagcgc gtaaaccaca ttgcaagact tgcatcattg aagatctttg cgaatacaaa
    32401 gacaagaatt tactttaaga caaagccata atgaaggaaa atctaacgat aatgaacaat
    32461 ctatgcctca taaaaatgac tggctgttgt atttcaaatt cattctatca aatcatccgg
    32521 gttgagatta gcttttgtac agttaaaaca caaaagttca attgaccatc agctcttata
    32581 aaaaatttca ataaagttca agaaaagtgc tctttccctc tggtatttcc caatttttac
    32641 tcataaaatg aaattccgct gttgcaggag agcattatga agtggttttc atcggtggta
    32701 aatggagtca aaacaattgg gtcaagcctg tgggaaggtt caaaatgggt ttggaacaac
    32761 gcctcattgt caaaaatcac gaccggcgta tttagttata ccgcaaatac aacttttcaa
    32821 gtgttagagc aggcaatagc cttacgtgat gctgttccca ccttggtcaa caatcctcag
    32881 gcgaagaaaa ttgcctatgg aatgggttac attcttgtta acgatgtttt accgttagtt
    32941 actttaaatt ttgtaaacaa tacggcacaa aattatttcg gagagggtta tgatgaggat
    33001 gcttcatggt atgcgcctta ttctcttttt ttatcgggat tagctctaac gaattatctg
    33061 gtcaaagcat acacctggcg gcaaggagtt caaactgtag cacatgtagc ggttcttgac
    33121 tcattaggcc cttcttcatt taattcgaat aaaaaatcac tccctccttc aatttgcgat
    33181 gaattggatt gtaactataa gcgtaaaatt aaaggttggg gaagagagcc tcttgtttta
    33241 atgggtaatg atgcactgac ttacggggtt agtttaattc cttatgtagg tgagcctctc
    33301 tcgcaagtat taagagtgta ttttaatggc cgctatatta ttcgtttaac tacaccagag
    33361 ctgtgtgagc gccataaata tttggccatg aaaccagagt ctgttttagg atttgggtta
    33421 acctttgaag cgacaagtat gttaatggac aaggctctgg aatccactgt gggcatgcct
    33481 ccgtttttat attacagggc tctgcgccat ttattgctgc tgatgcatat taatgttgct
    33541 gcgcatatgg agttgcctaa atctgatttg aaagacaaag cctcgtcttt tgatccgttt
    33601 aatgtttatg aacgcgtcaa tcgctttgta gcggatgtca tattttcagg tttaattaaa
    33661 aggataccca ttgatttcaa accggataaa gatgctgagc cgttaattcc attatctccc
    33721 gctctaaaat ttggcactat gatgttgaat atggatttgg agaaggaaaa gaaaacagct
    33781 cccggatttt ttagcaaagc catagataaa gcctatgttt tggttttacc ccctgttttt
    33841 cgcggtgcta atggcttaat caacgatcca attgtattta tgtattggcc tactattcgc
    33901 tcaggggcta tttctgctgt tgaatttgta gagggtgtgg ggaaaaccaa aacgatggct
    33961 actttggcat gggcaccaaa aactgtagca gccgccatca atctcaaatt tggaatccct
    34021 aaaaaagtaa cacgttttgt tttaatgtta agcaaagaag aagacttctg gaatctggct
    34081 acagcattca aattgtggtt tgagagacac aatatcaaag gtgaaccaaa attaattaca
    34141 ggctccaatc tggctttgaa tggggtgaca cctcttcagc ctactcccgt atctgttgaa
    34201 aaggaaccca tagtttctcc ccatctatta attacggaaa aagatactaa ggaagtagtg
    34261 ccagcaaaaa gcctgattcc tgttcgatcc caacctcaga gtggagttgt ggtgacgatt
    34321 agccctgata gtttattttc aaccaggaga cgtcgggtta atccagatca agaaaaagtt
    34381 gcgataacag aacttaacat gactaaataa gttttagagg cttttttgac aagagggcaa
    34441 cagtagtgtg ttgcccgtta aagaccctac ccagttctat cgatgatatt atgcaggttg
    34501 taaatggggt gcttgccgcc acttaaaata atttttttcg tttaaaaaaa tacagcatga
    34561 gtattgccag aagtaccatg gtaaccagaa tcatagggta accgtatcgc caatccagct
    34621 caggcataaa tttaaaattc attccataaa ccccagcaat aaaacttaaa ggaataaaaa
    34681 tactggcaaa caaggtcaga attttcatgg attcattcat gcgattattt atagtagaca
    34741 ggtatatgtc tagcatactt gccgacattt ccctatgcaa atcgatcgat tctaccaggc
    34801 gaatgcaatg atcatataag tcccggaagt aaagattata ctttttattg attaaccccc
    34861 catcgtctgt aagcaacaaa tggacaatat ctctcaaagg aggaatgatt tttctgaggg
    34921 taatggttct tcttttgatg gtgtataacg cttgtaactt aatcgtttct ggtttattaa
    34981 ttaattgatc ttccaaggtt tcaagcagtt gggctgaagt ttcaacaaag ttgaaataat
    35041 catcaacaat atagtccatg atcagatagg tcaaataatc acttccatat tccctggtta
    35101 acgagtgctc tccattaagg cgttgataaa tggaggataa attatatttt tcagattctc
    35161 tgaatgtgag taaaagtttt tgcttgatga ttaaacagaa ctgttctgta ttgtaagata
    35221 agtgatcggg aggggattgt aataatttaa aaacaacaaa aatataatcg tctacaatat
    35281 ccagtttggg tctttgtctg gtattaagca aatcctcgat aaccaagggg tgtatgttgt
    35341 attctgagca aattttagta attttctgaa tgtctgcaag acctgaaata tctatccaaa
    35401 tattatgtcc agcctcatag gcttctttca cttgtcgggt atcgaaggaa tctaaacgct
    35461 tataattctt tttgtcataa atatgtatcg ttacttgagt tggttgcggt gggtgttcgc
    35521 ctatatatac ggcagttccc ggtaatagtc ctgcttttgc tgaagccttt gttttatgag
    35581 tattcatgaa gttatctcag cttatttatt ttcttattaa cttatcattt agtcatcgtt
    35641 taagtcgaca gttgactact ctcgtttatc ttgctatgca aagggctata atgctatatt
    35701 tgcgacctga ttagcttaat aaagggcaat tattatttga agcttatagg gatgttgtta
    35761 atacttccat gtaaatcata aagaattaac cccctatagt cagtctctat caataaggca
    35821 gaatgagttg ttatatacta tggaagctct tgtcgtatga atcaatcgtt atcaccccaa
    35881 caatcccccg ggtttctgtt catcgcagtg agtatgataa cttgcctgat attattaatt
    35941 aacgtttctt ttaaaattgt tctactcggg gggctggtat ttgcaataaa cagtttgatt
    36001 tgtcccctta ttgcagcgtt atttttattc gcattgagaa attgtacctt caaagagcaa
    36061 cgccatttac ttaatatttg tttgatgact ttatatatgt tttgtattgg ggtttatgtg
    36121 ctcgtcaatt tacctgctgc tgaatacatg catgataatc cggcctatca agttattttt
    36181 gaagaaatac ccaagaaatt ttttgctaca actatcgctt ttgcactcag tttctatttg
    36241 cctcatttat tgttttgtac taaaaatgac agggttttat caactccaaa gcaatgcgtg
    36301 ttattagctg tgctgggcgg ggtaagtttt ttttgcctgg atttttatct attattttct
    36361 gctccacatg ctcatagttt caagcaaatt ttcatcgatt cattcatgat ctcgtctctt
    36421 ttactcctaa ttattggcgt agtttattta acatttttat tgaacgataa acaacataag
    36481 agcaaagcat taattgaaac cacagaatta acgctttttc caatttatca atatttgatt
    36541 tgcttcgctg tgaccgtgat gttggtttgt ttggcctgtg agtatcgaat catcgcgatc
    36601 aataaagact tggttttatc tgccagctgt attttctttc caattactat catcatcagt
    36661 tctatagttg gcgaattgtg gggttaccag gccaacttga aattagcatt ggctcttctt
    36721 gcggcacaat ttatttttga cgtgttattg atggggagtg ttgcactacc ttctcctcct
    36781 ttttttaatt tgaatccttt ttatcattac atcatgccac gcaggttatc agctgcatcg
    36841 ttgtcattgt ttgtcacttt tcttggtaat gcaatgttat tgcactattt aaagtattca
    36901 aaatggaaaa ttaaccgatc cttgcgaata ttaatcgcta atatatcagt caactcgtta
    36961 ttatgcctgg ttgactacag tttgctttat ggcggtattt atccttacga acaaattatt
    37021 aatttagcgg tgagtgtttg gcaatacaaa ctattgacag cgttaattgc tctcccggtc
    37081 attttatgga tgtgcaagat tttagaaaga aattattcat tagttgtgca aacagagagg
    37141 aattaaataa acaaatttaa tggttcattc tcttatattt tttgaaggaa tgagatatac
    37201 actaatagat tgagtaaggt aagtggggct cccacaagta taaactccag aaatccaaaa
    37261 cctctgatac cacgtttttc actattttga ataataataa tattacttgc tgcccccagg
    37321 atagagagat tcccggcaat agtacttccc gcagccaggc tcagcagggt tatactggaa
    37381 tgattatgac tcatcaataa aggcaaatat attgttacta atgggacatt agagatcaat
    37441 tggctcaggg cgatactcat tataaaaatc accacgatgc ggctgacatc aatatgagaa
    37501 tcactcattc cggtttggaa aaaaccgcta tcccatacgc tttgcattaa aataaaggtg
    37561 ctcgcaaaaa aaactaaagt tccccaatcc agtcttttta tatatttcca tcgatgcttg
    37621 ctaaaccaaa ttggtgcaga cgacaagagt gcaatataac taaaattcag ctgaaattct
    37681 gaataatgca ggaaatccgt caagattttg atcacagtca gaactaacat catgagcaag
    37741 ctcattttaa ccaaagtaag attgctacgg tcacttatca aggggaggac tggtttttta
    37801 ataggttctt ttaattcatt tttatataaa aaaagaataa aaaaataaac tataaacaga
    37861 tttaacaaag taggtacggc caggtgtttg aaaaactcaa aaaatggcga ggccatctct
    37921 cccttaatag caatcaataa attttgtgga ttgcctatag gactcatggt gcttccaata
    37981 gtaattgaaa atgccaatgc caacagcaag ggtttaatta aatttttatg tgattgacac
    38041 agttgtaaaa tgataggtgt acctataatg gcaatggtat cattcattaa caccgcggag
    38101 cttaaaccca gaatgaaaac aatcaacatt aacgtttgtt ttccattatt cgcataataa
    38161 aaaatttgat cagtcagaaa agcaaggtag ccactttcct ctgcggcttg tgaaatgaaa
    38221 aaaacaccaa acaaataaag catgacatcc ggctcaacgg cagtaaaagc gcgtgatgga
    38281 gttatctgtt ggcagagcaa aacagcaatg gctcctgttg ccataattgc ccaaattgga
    38341 atgacaactc taacaacctg gcgtaaagca atcgcaataa ggataatcag aagaatgatg
    38401 acagcagaag gcatttctgt cctcttttat ttaatccctc tcccttttta ccggcctttt
    38461 ccagccgtag atggtttttt gtcgtgattc ggttaatgtt aactcatcgg gcggcacatt
    38521 tcggcgtatg gtgctccctg ctcctattgt tgcgttagcg cccacggtaa ctggagcaac
    38581 taattgagta tctgacccaa taaaaacacc atcttcgata attgtttgat gcttattaac
    38641 cccatcgtaa ttacaagtaa tggttccagc gccaacgttt acattttttc ctaacaggac
    38701 gtctcctaag taacttagat ggctggcttt cgtgccttca tcaaaaatcg cttttttagt
    38761 ttcaacaaaa ttgccgattt tacaatggga agccaattgc gtccctgagc gcaagcgggc
    38821 aaaaggacca atgtggcagt cgtttgcaat gtggcagcct tccagtacgc tattagcgta
    38881 aacttcacat ccatcgccta atgtaacatt agtcagactg cagtttggac caattttgca
    38941 gccattacct aaaactaccg tacctgtaaa aatgcaatta atgtcgatat agacgtcttt
    39001 gccacaatat aattctcctc tcaaatcaaa gcggttggca tcagccaggg tcgcgccttt
    39061 ttccatgagt tgattggctg ctctttgctg ccagatgcgt tcgagttgct gaagttgttg
    39121 gcgattatta atgccctgca cctcaaagct gttcttggcg gttaatgacg taatgggtgt
    39181 tttattttgt acagccaatg agataatttc agttaaataa tattcgcctt gtgcgttact
    39241 attacttagt tgaggcaacc atttcttcag attatttgcc aaagtgcaac aaaccccgga
    39301 ataaatttct tttatattct ttacttgctc atttgcatcc ttttcttcta caatgctata
    39361 aatttcacct tggttgtttc tgataatacg cccaagaccg cttggatttt ccagttctgc
    39421 aaccaggagt gctaaaacag aatgatctgg attttgacgt tggctgcatt caattaacga
    39481 ttgaagagtt cccacttgta tcaggggaac atcggcagat aaaaccagaa cgtatgcatc
    39541 gtcagggata tggggcatgg cttgcaaaac agcatggcca gtccctagtt gctctgcctg
    39601 atgcacccaa tgaacaggta agttggggag tgaactttta atttgttcac caccatgacc
    39661 ataaatgaca tgaattgcat cgggatttag ttgctgagcg gtttccacga cgtgggttaa
    39721 cagcggtttg ccagccaggt ggtgaagaac tttgggcgtg tccgaataca ttcttttacc
    39781 ttgccctgca gctaaaataa taatttgtaa attcatgaga tttgtgtttt cctaagttaa
    39841 atatcataat cggtgctgat ttaaacagtc tgccccaatg ttatgaatga tgtaaagagc
    39901 tatatgataa ataagttgca gttaaaggag tagagcaatg aatatatttc taaaggtttt
    39961 aaagaaatta atgaaggaaa aattgagtga gttatccgcc aatttgaaat ggaagtcaga
    40021 aaccattggg tataatacca aattatcaat cgatcaaaag aatgtcattg ataaactcga
    40081 agaaagtatc gttcttttta aagattcagg taatgacgaa gcggatttgc tgtcaatcaa
    40141 tgcattgata gatactgcca gaatacaaat tcaatccata agagaatcct atggtgagcc
    40201 gagagataaa ggggaatctg taacctgtct caataatttg aaaaatcatt ccaacgattt
    40261 tcttggcaaa ctaaagaaat ttgattttga cctgttaaat aaagcttata cagaaacacc
    40321 tgaaaatatt gtctattacc atgctgctat ctatctggga gatgaaattt ttagccctag
    40381 aacagggata gattacgaaa tccgcaccaa aaaagaagtg caattggcag taaggctgca
    40441 ggcattaagc gagcgaatta atccaagcca caatttggaa gaacaaagaa aaagagcgtt
    40501 gcaagtgttg caagatcttg cccaggacaa tcaaaacgct atcaagaaag ataaaggttt
    40561 ttctcttcca gggttaagct tttggggtgt ttcagtagtt acaccatcgg attggtttag
    40621 ctcaggagaa gggcggtttg gcgttgaatt taatacggcg gttcgcacca ttcagggaat
    40681 gactgaaagc cagtttgaaa gaccgacagt aaaacaaaca actgcaaagc aagaagagac
    40741 caaaccatcc gatgtaaaaa aaacagaggc aacagacgag gaggatgaag aacatttatc
    40801 tgaaaatact catacattgt aagttacttt caatgggtgt attttgattt ctctgcctcg
    40861 aatgcatacg agaggtctcc aaaagatatt ttctgttgga gcaaggagat ccctcgttct
    40921 taaatctgca gttcaccaca caaattggtt tcaatcagtt ttctgatttc agggatttcc
    40981 agattcttgc ttaataaata cagcaatttg caatgagctg cttcaggtgt catatcatga
    41041 ccgctaatca agcctgcttc ttttaaagta tgtcctgttg cgtattgatt catttccact
    41101 ttcccctggt ggcattgggt gcaattaata ataataaccc ccctgccgca agcctcctta
    41161 agcaaactta aaaatcttgg atcattgttc tgggcattac ctgccccata ggtttcaagg
    41221 attaaacccc gtaaaggttg atttaaaatg tactctagaa catcagaggc aaagcctggg
    41281 aacaggcgga agtttgcgat aaagtgagga gtaatggttt gtagatggaa cggtgctttg
    41341 gggcgtggca ataaacggtg ttgatgtaac tcgatatcga taccaatcga tgccaagggt
    41401 gggtagttgg gcgaatcaaa agcattgaag cgttgggcac tgattttttg ggtgcgattg
    41461 cctcgaaaca aatgttgatt gaagtagatg catacttcat ggataggttg atgagcacaa
    41521 agccataagg acgtaatgag gttatctgtc gcatcattac gaatctcgga taaaggaatc
    41581 tgtgaaccag tgactatgac gggttttccc aaatactcca acataaatga caaagcagaa
    41641 gcagtgtaag ccatggtatc agtgccatgt aatacaacaa atccgtcaaa ctgttcatat
    41701 tcgtgagcaa tatctctggc aatcctgttc cattccgtga cggtcatatt agaggaatca
    41761 agtagcggct gatattcttt gataacgtag tcaggcattt cttgatgttt tagagcagga
    41821 atgtctgcca acgctttttg aatgaatcct ggcgaaggct cataaccatg cgatgttttt
    41881 atgctactga ttgtgccgcc agtattgata attaaaactc gttttttcat gcggatttat
    41941 aatcaatgat gtgttattgt ttcgataagt atagcgaaaa cttggctata ccaataacaa
    42001 ctaatttcag gacttgccaa aagccccaac ctctttagta aaaaaagtgt ttaattcggt
    42061 catttagttt atcgaaactc cctcgttaaa aacattaaag ttacctgcct ccaaggtatt
    42121 gagagtcatt atgatgagta atttttgttc tataataaat caaaaaatgc aatatgaaaa
    42181 cagggaataa attagtccaa ataatattta cagatttaat tgtaagctct tgtactttgt
    42241 ggaaaaatca agttattaac ggtttattct aagcagtgac atcatggtta tcgatcgtgc
    42301 cggatatcgc ttgaatgtag gtattatact cgtcaacgat tccgatagag ttttttgggg
    42361 gagacgaagc gggcatgacg cttggcaatt cccgcaaggt ggattggcac caggcgagac
    42421 ggccatgcag gcaatgtatc gtgaattgca tgaagaagtg gggttagata aaggtgatgt
    42481 tgaaatattg ggttcgacca ggcgttggct aaagtatcga cttccgaagc aatatttgcg
    42541 tcatggcagt gagcctttgg tgattggcca gaaacaaaaa tggtatttgt taaagcttgt
    42601 aacaagcgag caaaaagtca gattggattt gagtgattct cccgaattcg atagctggcg
    42661 gtgggtggat tttcatgagc ctgagcaaca agttattttc tttaagaggc aggtttatat
    42721 tcaagcgtta aaagaattag aacctttgtt aaaaaaagaa cgccgtaccc cttatggctt
    42781 aaaacgtaaa agaggtaatc aaagagctta gatgcttaaa atacttaaac ggatagtaca
    42841 agatgttacc gctgccagtg atttgaaaga agcacttggt attttggtgc aacgtattaa
    42901 tacagccatt aatgcagagg ctgtttccgt ttatcttatt gataataaaa atgctgaata
    42961 cgtattgata gccacggaag gtttgaacaa gcaggcagag tttagagtca gaattgctct
    43021 tgattgcggc cttatcgggt tgataggacg aagagaggaa cctatcaata tagaaaatgc
    43081 tccctctcat ccggattttt atcataaccc attaattggc gaagatcacc tgaacgcttt
    43141 tttgggcgtc cctattattc aacatagaaa attatacggt gttttagttg tacaacagct
    43201 tgaacaacgt cattttgatg atgctgaaga atcatttctt attaccttgg cggctcaatt
    43261 aggcgggatt attgctcatg cagaggcaac aggtgaattg gccgagttaa cacaacctaa
    43321 aaagggtata ggagtcgaca aggtagaagc ggcttatact gcactgactg gaataggcag
    43381 tgttcctggt ataggaattg gtactgccgt ggttgtgtac ccgcctgcgg atattgatgc
    43441 tgtccctcgt aatccagtgg aagaattgga agatgaaatc aatgcgtttt atgaagcttt
    43501 ggaaacgaca agagatgata tcaggcgttt aagtaaacgc atgaaagaaa cggttgctga
    43561 ggaagagcat gctttattcg atgtgtattt aagaattttg gatagggata gcttgggtgc
    43621 tgaagtagag catgtgattc gtgaagagaa aatcagcgct caagctgcct tggctacagt
    43681 gatcaagaaa cacgtgcagc aatttgaaag catgggtgat gattatctac gcgaacgagc
    43741 cagcgatttc cgtgacttgg gccgaagagt tttggcggaa ttacaacgta cccaaagaga
    43801 agaaattgtt tatccaaaga gaaccatttt aataggagaa gaaattactg ctgccgctct
    43861 ggcagaagtt ccagaagggc aattggctgg tgtcgtttca gctaaaggct ccaataactc
    43921 ccatgttgct attttagcga gagctctcgg tgtacctact gtgatgggat tgagaggatt
    43981 aaaagtagaa ttggtatcgc gccgggcttt gattgttgat ggctattatg ggcatgttta
    44041 tatttctcct tccaaatcat tattagcgga atttaagcaa ttagctcaag aggaacagga
    44101 attaaaccaa agcctggtaa gcttgcgtga taaaccagct gaaactactg ataactacag
    44161 agtctctttg caagtcaata ccggcttggc aatggatgcc ggtttatcaa tgagtgttgg
    44221 cgctgaagga gttggtttat accgttcaga agtaccattt atgagtcgag atcgattccc
    44281 ttcagaagat gagcagacca ttatttacag acagaccctc aaggcctttg ctccacgttt
    44341 ggtgactatg cgtacactgg atattggtgg cgacaaaatt ctaccttatt ttccagttga
    44401 agaggaaaac ccttatttag gatggcgtgg aataaggata actcttgatc atccagatgt
    44461 cttcctgatt caggttcgtg ccatgatgcg tgccagtgag gaactggata atttacgtat
    44521 catgttgcct atggtcacta cacttagcga agtagaagaa gccgtttatt tgattgaaca
    44581 ggcatttgaa gagctggtgg aagaggggtg cgaaatagta aaacccaaaa ttggtgtgat
    44641 gattgaagtg cctgccgctg cttatctggc aagagaaatg gcgaaacgag tggattttat
    44701 ttcggtaggt agtaatgatc ttactcaata tttattagct gtggatagaa ataacgcccg
    44761 agttgccgca ctttatgatc ccttgcatcc agccatgtta cgtacattgc ttaaaatagt
    44821 ggaaggaggg catgcggctg gtgttgaggt gagtatttgt ggcgaaatgg ccagcgatcc
    44881 tttagcggtg atcttgttaa ttgctatggg ttttgacacc ttaagtatga attccgccag
    44941 tttacccagg gtgaaatggg ttattcgcaa ttttgctatc gcgaatgccc gtaaaattct
    45001 ggcagaagtt ttggaatttg aacatcctgc tgaaatacga tttcatttgc aaaaggccct
    45061 agaagaggaa ggattaggag gattaatcag agcaggaaag tcatgatatc cggtgagtta
    45121 ctgattaatg gagtgattta catcattatt ggtgcttttg caggaataat ggcgggtgct
    45181 ctgggcgttg gtggcgggct tattgtggta ccaggactcg tctatatttt tcaacactcg
    45241 caggtgattc cagaagatat ccttatgcat gtcgctgcag gttgttcact ggcggtgatg
    45301 atttttactt cttatgcatc cttaaaagca cataataaaa tggatgagat actcttaccc
    45361 ctttttaaga agttatgtcc gggtttggtt cttggtacta ttttaggttc cataatggct
    45421 tcttttattc caacccattt attgaaaata atatttgggc tgttcttgct tggcgtagct
    45481 atgaaaatgc ttcttgatgt ccgagtgagc cacactgata aatttccggg atattggctt
    45541 aatactttag tcagcagttc aataggctcg atatccggtt tattaggggt gggaggagga
    45601 gtcatcatta tcccttatct gacctattgt ggagttccag tcagaaaaat tgccgcagta
    45661 tccaatttgg gcacttttgc agttggtcta gtgggtacaa tcatgtttat gattactggc
    45721 acattagaca tgacaaaaat atcgtattcc atagggtata tatattggcc agcagtttta
    45781 ggggtagcaa ttccaagtag tttgattgca ccatttgggg cgaaattaaa ttatctttta
    45841 cccgtaaatt atttaagata tggatttatt gtgattttaa ttaccaccgc aattcatatg
    45901 tttttgtgaa aggaatttga ataaccatgc tcacctatcc aaatattaat ccaattgctt
    45961 tttctttcgg gccattaaaa gttcattggt atggtttgat gtatttaatc ggttttattg
    46021 gcgcctggct actgggttat tggcgcatta aacattataa attgaactgg aataacgacc
    46081 agttaagtga tttgatcttt tatagtgccc ttggtgttat tcttggggga cgagtaggtt
    46141 acatgttgtt ttatgacttt caggagttta tccaccaccc ctgggtatta tttaaaatat
    46201 gggaaggcgg gatgtctttc catggtggtt tgcttggagt agtgattgcc gcctggctct
    46261 tttgccgtaa atacggaaaa actttcttgg aggttggaga ctttgtcgct cctctagttc
    46321 ctttaggttt agcggcaggt cgcctgggaa atttcattaa tggggaatta tgggggcgtg
    46381 ccactgatgt tccgtggggt atgatatatc ctcatgttga tgatcaacca agacacccct
    46441 ctcaattgta tgaatttggt ttggaagggg tcgctttatt tattttgatt tggtgttatg
    46501 cgtcaaagcc gagacaacaa gggagagtga gcgctttatt tttaatggga tatgcgattt
    46561 gcaggttaat tgctgaaagc tttaggcaac ctgattcgca attaggtttt gtggcatttg
    46621 gctggctgac aatggggcag gttttatcca tccctatgtt attaattgga atttggttat
    46681 ggtgggctaa gcgatgaaaa catatttgca attgttggag catattttac aacaaggggt
    46741 agaaaaatcg gatagaacgg gcactggaac attatctgta tttgggtatc aaatgcgttt
    46801 tgatttgacc aagggattcc cgcttgtcac tactaaaaaa ctacacacga gaagtattgt
    46861 tcatgaatta ctatggtttt tgcgtggtga taccaatatt tcttatctta aggaaaacgg
    46921 ggtaactatt tgggatgaat gggcagataa taatggtgat ttggggcctg tctatggaaa
    46981 gcaatggaga agttggccaa ctgctgatgg acacaccatt gatcaattaa gtgatgtagt
    47041 gcaacaaata aagagtaacc ctgactctcg tcgcttgatt gtaagcgcct ggaatgtggg
    47101 tgaattggat aaaatggctt taatgccttg tcatgccttg tttcagtttt atgttgcaaa
    47161 caacaagtta tcctgtcaat tgtatcaacg ctcagccgat gtatttttag gagttccttt
    47221 caatatcgct tcttattctt tacttactca tatggtcgcc caacaatgta atttggatgt
    47281 ggctgagttc atatggaccg ggggagattg tcatttgtat cttaatcatt tggagcaagc
    47341 gcaaatccaa ttaacaagag aacctttgcc attgccctcc ttaactatta agagaaaacc
    47401 ggcttcttta tttgactatg cttatgagga ttttgaattt gtgaattatc aatcacatcc
    47461 cgcaattaaa gcacctattg ccgtttaatt attatcagta ttggattttt attgccaatt
    47521 atggtttaat attgcctcaa gtgtttattt gaggtccagt gctgtgaaaa aaattgtagt
    47581 gtttgtcttg ctggctgttg tttgtagcgc aggctttccg agtaaattga gtaaatttct
    47641 gaataaaatg gatgctgagc aaaaacaaag agacgctcag gaatggcaac aagatatgaa
    47701 ctttggagat tatgcttttc gtttgaaaga gcgctatatc gatgatcgcg ggcaacgttg
    47761 tcgtgattac gaattcagag gacgctccaa cccctatcga catggttatt ttactgtgtg
    47821 tgatgatcgt taatttggct ggaaatactc tttctgtata ttaaattatc gtccgctccc
    47881 tgacggtcgc ggctcagttt tagttataat ctgagccgcg accgtcaggg agcgattgtc
    47941 caaataggcg ctacactaaa ttcctaataa tatcaggcaa aggcacggat ttattttggg
    48001 tataatcaat ccaaactatt ttactcactc cttgtgccat aagcgtttct tcctgataca
    48061 aatcatgatc cataatcatg ctggaattgc caagactatg tgccttgctg tgtatggtta
    48121 cagtggcagg ataaacgatt ggtttcagaa aggtacaagc cacatggatg acaacaggac
    48181 ctgtttgacc tgtcattttg atgtcaagct cccggagcca atcaatccgg gcttcctgaa
    48241 aatagtcaaa ataacgagca ttgttaacat gtcctaatgc atccatatcg ccccaagcga
    48301 tatcaaaagt ttttttatgt attattttgt ttatttcagt catgataagt ccataggatc
    48361 aacatccaca ttccagcgga tgccattact taatttattc attgttaacc actctctcaa
    48421 ttgcgtcaat gaacttttta gaacttttct tgaaggggat ttaattaata gttgcattct
    48481 atgctggtta gcttttcgtg gcaaaggagc tggcgcaggg cctaatacct tgagaggatg
    48541 ggcctgaatt tgatctttgg ttgcttttag gaattgagcc accttgttga cggttttgcc
    48601 ctgagctctg atgaccgcca aaaactgaaa agggggcatt tcagcctctt gtcttgaaat
    48661 taataaagcg tttgcaaatt catcataccc atgctgaatg agtagattga gcaggggatg
    48721 atcaggtaaa taagtttgaa tcaatacttg cccggcatgt tctgctctgc cagctcgtcc
    48781 agatacttga gttaataatt gtcccaaatg ttcaatagcc cggaaatcct ggttgtaaaa
    48841 acccgcatca gcatctacga caacgaccag ggataggcgg ggaaaatggt gtcccttggc
    48901 tagcatttga gtgcctatga tcagctgagc ttccccttta ttaattttat ccaaatggct
    48961 gtttagagaa ttttttttac gaacctcatc tcggtcaatt cgtaaaacgt tcgtatctgg
    49021 aaactgatgg cttaaaaatt cataaattct ctgggtgcca gatcctacgg ggattaattc
    49081 cttgctttgg cagttattgc aaaacaaggg tattctttgg gttaatccgc aatgatgaca
    49141 aatcatttgt cctgcctgtt tatgcaaggt taaatggcta tcgcatgctt tgcagtcaac
    49201 catccatcca cattgatggc agagtaatac tggagcaaag ccgcgtcggt taatgaaaac
    49261 aagtacctgg ttttgttgca gcaaatgatt ttctatgatt ttaagggttg ggagagccaa
    49321 gccatgctgt accatttgcg atcttaaatc aatcagttga tagtgtaatg gcgtagtgga
    49381 ggcagcttta tgcgttaagc gtaataaatt atattttttt tgtttgcagt tgtggatggt
    49441 ttccagactg ggagtggcag taccaagaat gataggaata ttggcaagat gagcacgcat
    49501 taaagcagta tctcgcgcag aataacgcac cccctccatt tgtttgagtg aactgtcatg
    49561 ctcttcatcg atgataatta aaccgagatt gggcagagga gtaaaaacgg cagaacgagt
    49621 tccaatcacc attttaactt tattctcttt tgccagctgc caggcaattt gcctttctga
    49681 ttcattcaaa ttggaatgta atacggttat gggatggtta aatcgggctg taaaacgaga
    49741 taaaagctga ggggttaacc cgatttctgg taccagcacc aatacttgtt tgccttgctc
    49801 caatattttg gcgatgacat gcaaataaac ctctgtctta ccacttccgg taacaccttg
    49861 cagtaaaaag cattggtaat gatggatgtg ttcgaaaatg gaagctacgg caacggcttg
    49921 ttcaggattg agtgctaacg gtggactaag tttttcagga attttggttg gcaagataac
    49981 ttgctgtgac aggttaatgg cttgacaagc aagtagtgcc gcaatttgag ttccgttata
    50041 gccatgttgt tttagggtct gtttggaaat tcctttctgg tgcctgctta aaaaatcaat
    50101 caattcatgt tgtttttttg cttttttcga gatgagtgtc ttcgcctttt caacagggat
    50161 attcaattga taaaaatctt ctgtaggtag tgagcaaggc aaacccaggc gatatttttt
    50221 gggtattacc aaaggaatca cttcagataa tggtgactga taataacttc ctacccatag
    50281 acaaagctct aagagatctt caggaaccag aggtttctca tcgattattg aagtgatttt
    50341 ctttaacgaa tggcccgttt gctggccaca aactttattg attacaatgc ccactcgggc
    50401 ttgattgcga aaaggaaccc atactcttcc gccaatgcat ggagttaaat ccagggcttc
    50461 gtaatcaaaa caatctcgat tggtatgggg gatacaaact tgataaatat cgttcattta
    50521 acttctaagc cattcatgac tcgctgattg gccgggcgcg gaaaacactt tgactactac
    50581 tttttcgatg cgatgtctat ctaactcctc tttttcttta atcagttcat taacaaacca
    50641 gcgtgataac tcttccacgg taatattggt taagggcatt aaggtgacat cttctttaag
    50701 aaaagggatt tttttcttat tgaaggtgaa ataataatat tcttcgtctt ccgagtattc
    50761 taaaaaagga gaaaattgtg gcataagaaa agtttgattt aagtagcggc ataatttatg
    50821 aatccgctct ttgtaataac ggtaatcaaa agtcatacca ttttcttcta cccaggtagt
    50881 tagagctaaa taaacagaat acatatgacc atgcaaaggt tccctttctg ttgctgaaaa
    50941 gatggtggta tgcccggcag aaaacttcat ggattctttc tgtaattcca cagtagtcag
    51001 atattttttc ataattataa attcactcgc ttatcattta tcagaaaacc atttaaatca
    51061 atttccagat ctttggttaa cgcaatgact ttgaatagct ctcccatttc actcggttga
    51121 gttagctgtt taatggcctg ttttgccgca aacagttcct gttcatcatc aagagtatgg
    51181 attaagctta gcaaaccgtt cgccagcaaa aaagaggctt gattagtata ccctgcaata
    51241 tgaaatcccg cctggtgccc tgcctcagca acatgagtaa aatcaacatg tgcggtgaga
    51301 tcttgttctc ccgggttaag taaaggattg ggatgagaat aatgttggta atgacacatt
    51361 aacgtgcctt gattacgatc agggtgatag tattcatggc gagggaagcc ataatcaata
    51421 ataaacaaag cccctttttt caacatatga ctgcaattca acaaccaatc atcaagaaac
    51481 aaattagcct cagtaaggta aggataaccc agtggtggta aatgattttt gatataggcc
    51541 aatagtcgtt gattttgtac tggcttgaag atttctgcta attgatcatg ttcatccaga
    51601 gtgacatagc tttctaggat ttctgtttca gattgcataa agcgataaac tggcatggca
    51661 tccagtactt catttgctat tattactcca ttaaaaggag cctctggcca tttgtccagc
    51721 cagtgaatca agccagccaa ttgcgggatt ttttgttgaa tcaattcttg ctgacgatga
    51781 cgtaagttag cacttacttc caggattaaa tatgattcag gcaagcactg taattgttcc
    51841 aaatgagtta atgtatcaat acagagcttt ccagttccgg ccccaaactc gaatagtacg
    51901 ggggaatcca gcaagggaag aatttgttgg aattggttgg ctaaagtttt gccgtacaag
    51961 gaggttaatt caggagcagt gataaaatca ccgtgttttc ctaacttttg cagtcctgaa
    52021 gtataatagc cctcataggg ggcatagagt gcttgctgca taaagtctat aaaagggatt
    52081 tctttttgca gctttaactg gtttattaga gtttgtttta aattcataat cacttaaact
    52141 atcgttagac ttctttttaa ctcgctgtga acaagagggt gcgaggagaa acaaagaaaa
    52201 tactgaagtg tacacaataa tacttgagga ttcgagcatc ctggctgatt actttttgca
    52261 gtgaggttgc gaaaggagtc tgttaccata tggggaacgt accttgaatc agacaaacac
    52321 gcaagaagcc aaggttgctt tagtgactgg aggcgctaaa cgtatcggcg cagcaattgt
    52381 taaaaaatta catctctctg gatatcaggt agccatccat tgtcgttctg ctttgcatga
    52441 agccgatatt ctggccaaag atttgaatcg gttgcgtacg aatagcgcca ttgtcatgca
    52501 aaaagaatta actgagcctg atgcagcaga agagatcata tccaaagtga aacattgggc
    52561 cggtcgtttg gatttgctgg tcaataatgc ttctgttttt aaacgaacgg attgccattt
    52621 tttgaatgag gcagactggc aatatctgtt tgcagtgaat gtccaggctc cctttctact
    52681 gagtatggca gcttgtcctt tactagctcc atgcgagggt tccattatta acataactga
    52741 tattcatgca gataagccac ttaaaggata ttcggtatat tgtcaaacta aagccgcatt
    52801 ggagatgcaa acgaggtcgc ttgctcgtga gttggcgcca gaaataagag tcaatgcagt
    52861 agcacccggc gccattgcct ggcccgagaa tgacaatagt ttaactgcgg aagcacaaca
    52921 aaaaattatc gagaaaacac cattaaaaag gcatggcagt cctgatttta tcgcgcaggc
    52981 tgttctctct ttagctgaaa atacattcat cactggtcaa acattgaaag tagatggagg
    53041 tagaagtttg acaagctaga gttgtatact ggatttcatt tcaaatgctc aatttactat
    53101 atagggatta ttattatgtc aaaatggatt ttattatctg gctccttttt tttatgccta
    53161 ttctctttaa gtgttcattc ccattctttt gataaagagc agttagtgca aagatgccag
    53221 attcttcatg atgaattaaa ggagttggaa tctcatcagt acaatggggt atgtcggcat
    53281 aaactggcat tagctgcaaa taagatattc tctgcaaaaa ttagaattgt ttatgagaat
    53341 tacaaaggcg ctaaacaaga tctttcagta tcaatgaata atatgaaatt tgcagaagat
    53401 atttcttgtg tttttaagtc ggagattacc aaggccagaa tggaag
//
LOCUS       DATNSN010000005        50933 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.005, whole genome shotgun sequence.
ACCESSION   DATNSN010000005 DATNSN010000000
VERSION     DATNSN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 50933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50933
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.005"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            172..2148
                     /locus_tag="VAH76_000226"
     CDS             172..2148
                     /locus_tag="VAH76_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1384888.1"
                     /translation="MNYRPDIDGLRAIAILLVLIYHGGLSLFPSGFIGVDVFFVISGF
                     LITTIIHESLNKNDFSFFDFYNRRLWRLQPVFVALIVVTTLLSLLFYLPDDLIEFSRS
                     ARKASLFISNLFFNKTTTGYFSPDTHQLPLLHTWSLSIEWQCYLVLPLVMYGLYRFLN
                     KGQVIYVVYGLVILFFLLALHYSQAAPAQTYYQFSSRIFEFLIGSCIALGPRIEFSIN
                     KSILVILGSSALFFIIYTATLDNILLGYPNWHTLFVCLATGLLIALGTNFPSLAIVKT
                     LSFRPLVFIGVLSYSLYIWHWVVFSILRYQNIEERSAVLFIVYGIVFVLAYLSWRYIE
                     KPTKKFRRMPFRYTVVILLLIPVLFSHLSSLVIKSHSGYPQRFNQELVRVYKQLKSYE
                     SPYRALCIDNTRADNAWKCKIGAKEQGGRTGLMIGDSFSNHYWGFMDVLGKAAGVSIL
                     AQGTSSCISLPGIYLYDWWHFKDKIYQDCYEQTEKYYRMIQENHYDYVIIGQVWNNYV
                     SAHIINQPGDDYSAELTKKRLADALDRALGVIISSGAKPVLIESTALTHGNLHQCFFK
                     HIKLRQSYNSEECRFTLTSSDNEIWLNQLFDNMRRKYPQLVIIDPKQVQCRDNVCYSD
                     LNGIPVYRDEGHITDYASYQLGYLYLREFENPLI"
     gene            complement(2199..2465)
                     /gene="yhbY"
                     /locus_tag="VAH76_000227"
     CDS             complement(2199..2465)
                     /gene="yhbY"
                     /locus_tag="VAH76_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO1384889.1"
                     /translation="MDTSFKQSLKAKAHHLKPVVLLGAKGLTEAILAETNVALLAHEL
                     IKVKINGAEKEDRMQMAEELCEQLHAELVQMIGNTLVLYRKNEE"
     gene            2568..3188
                     /gene="rlmE"
                     /locus_tag="VAH76_000228"
     CDS             2568..3188
                     /gene="rlmE"
                     /locus_tag="VAH76_000228"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEO1384890.1"
                     /translation="MSRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKES
                     LIKPGMTVVDLGAAPGGWTQYASEKMKGSGRLVALDILPMDALPNVEFILGDFREDNV
                     LQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
                     KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL"
     gene            3311..5221
                     /gene="ftsH"
                     /locus_tag="VAH76_000229"
     CDS             3311..5221
                     /gene="ftsH"
                     /locus_tag="VAH76_000229"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO1384891.1"
                     /translation="MVKNLFLWLIIAIVLVSVFSNFGPRNSVAEKISYSQFLQEVDQG
                     MVNSVTIEDNKIIKGVTKNNKRFVTYMPMQDNALLGELLKSKVDVSGQEKQQESFLLH
                     LFINWFPMLLLIGVWIFFMRQMQGGGGRGAMSFGRSRARLLGEDQVKVTFADVAGVDE
                     AKEEVKELVDFLRDPTKFQNLGGRIPRGVLLVGSPGTGKTLLARAVAGEAKVPFFTIS
                     GSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEGNEGVIVVAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILKV
                     HLQKVPVDSHVEVKAIARGTPGFSGADLANLVNEAALFAARANKRKVGMIELDKAKDK
                     IMMGAERRSMVMDDNEKKLTAYHEAGHAIVGLSVPEHDPVYKVSIIPRGRALGVTMFL
                     PEQDRYSHSKRRLESQLCSLFGGRIAEELIFGPESVTTGASNDIMRSTEIARKMVTTW
                     GLSALGPLTFGEEEEEIFLGRSVNKHKEMSDRTAQQIDDEVRAIIDRNYQRAKEILET
                     NIDKLHLMAQSLIKYETIDTNQIQEIMSGKEPTPPEDWGSTKPMDKAESVNDAPAKPI
                     NGETIENPAEGH"
     gene            5254..6129
                     /gene="folP"
                     /locus_tag="VAH76_000230"
     CDS             5254..6129
                     /gene="folP"
                     /locus_tag="VAH76_000230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO1384892.1"
                     /translation="MNSEQFLGWLERKSQPASSLVFEKPLIMGVLNVTSDSFYDGGKY
                     LSVDRAYEQAIQLIACGADLIDIGGESTKPGAPPVPLDVELSRVLPIIKQLRAFTDVC
                     ISIDTNKPEVMEAAINAGANVINDVYALRRDGALNVAAQLDVPVCLMHMKGEPRTMQE
                     NPSYPEGIMKELQHFFEERIDKCQKAGLNRNKLILDPGFGFGKRVQDNLQLIYHLNEL
                     HSFGLPILLGVSRKSTIGAVLNTEVEQRLIGSITLGIYAALKGTAIIRTHDVDETNQA
                     LTMINAIYQAAKEIS"
     gene            6193..7560
                     /gene="glmM"
                     /locus_tag="VAH76_000231"
     CDS             6193..7560
                     /gene="glmM"
                     /locus_tag="VAH76_000231"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO1384893.1"
                     /translation="MSQRKYFGTDGIRGHVGLSNINPEFVLKLGWAVGCVLANGARKK
                     VVIGKDTRVSGYMLESALEAGLSAAGVDVALLGPMPTPGIAYLTQTLRANAGIVISAS
                     HNLFEDNGIKFFSADGGKLPDSVELAIEAQLEKQLQTVPSVKLGKATRINDAAGRYIE
                     FCKSTIPSLSRLSNLKIVVDCANGATYHIAPNVFSELGADVVPIGIKPDGFNINQECG
                     STAPELLREKVIAVGADIGIGLDGDGDRVILVDSLGNLVDGDQIIYIIAKDRHQRGVL
                     HGGVVGTLMSNYGLELAITSLGVPFQRSKVGDRYVLETLREKDWKIGGETSGHIVCLD
                     KTTTGDGIVAALQVLSIMVKQNKALHELTAGIQLLPQTLVNLKTNNAALLASNPDVIQ
                     AVKNLEKHLNGEGRVLLRPSGTEPLLRVMVEGANASIVKQQAQMLCDDISQIDKKLTE
                     SLPST"
     gene            complement(7630..11076)
                     /gene="lepA"
                     /locus_tag="VAH76_000232"
     CDS             complement(7630..11076)
                     /gene="lepA"
                     /locus_tag="VAH76_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LepA"
                     /protein_id="HEO1384894.1"
                     /translation="MNEIILHLMNEISALTPPINPLNLKQIFSSTLLPEGKAPVEVES
                     QLNLLLLIKKYVDEYSSLAEEQEEYKRKKHKLDEVMKKGEELQRKISDLAKKIEHAKN
                     ELNPLYKEIEPKANEVLSKQNPLAPPYKFPEYGEVQKSIVVGHYSYLFKTSKEIETTR
                     AKLKLVLLKNKWNALMTDLSQLGIQPPVEEDAVDSLQKFIAAVELEIKKLTRACEKWD
                     ELQKRFAKQLSATDINKKETELEKQIELLLKDLGRIETAVTNHSLAPDLKAELIRKFQ
                     ESEDPQGLIQEYQNQIDGLLSNFNPSSWLSWYSDSNYSDNQQKLKNSVSFLQLLAQQK
                     ELKIKHHELVKQQELLIEAIPEIPSNEEDISIYKKLVPDAIDLINEIPIESIPNFVLP
                     SGLSYDSSPADFYLVLLILMPKISEKKEQFSQALEKLQEMPALGKQIQQLRSGYDLPI
                     TMDVQLPSLHEAEENKDSLNEEDPLKEELKSQIKLCQSYLETAKQIDLLMKDHGQTTN
                     EAKALIEQLRLISNQPLKNEDINSIKEDLSTLANQIQSLVSELNQLPLPNLNDEKIDP
                     PLEKQEIAVDKAIPLTVELMNPAQVIQIGNDSHIEKQQLDNEEETPVVLESLRPRPVI
                     HKESESITQKLQVDNGHEMPVVLDSTCPPQVIHKELESTTEKLQVGNGHEIPLVLEST
                     RSPQIIHPEPESVGKKQHVEVEQEIPVALELIRPSSMVEKDSVATVEKQQATERQETP
                     IVLESTRPSPMVQKDTGLSGEKQHKEIEQEIPVIPELTPRTQVVRQDSESSLEQQVPN
                     GQETLAFVKSSPSEPLRPSSPEIPVISRKPNGLSLFNGHDELSEDNILDFFDEASISS
                     EEDSETLAIDKGKVNSLGIDKAFKEKPVSTGHELDKLSNPQHQESAPSISPLLPPSSI
                     LLKQKLDSFHVQNMEYIKHHSEEIQLWYKGLYDAVQSSCVNETLGLKALHLLKDILFE
                     LKNQNDLSALLAYKRMCPNPLQDIQNILRLKPALPILDESIDEEQQLKNWPEELQKFH
                     QQYVKLKKEYPLEAELFIQAIHSLISIKHLMELPDAKTSNREAMPLITQDPRYEPLKR
                     HRGFTKAWEYIEDFFRMLIGKLTGQDEYEYSKRPCFFKTRSHRLLEEADTMLHSMAPT
                     SS"
     gene            11320..12069
                     /locus_tag="VAH76_000233"
     CDS             11320..12069
                     /locus_tag="VAH76_000233"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO1384895.1"
                     /translation="MRQKIVAGNWKMNGQIQQVTELVSQIEELIGFDCAAQVAVMPPS
                     IYIPKVRDCLRTGRIVVGAQNVYPKDYGAYTGELSAPMLKDFDCRYVLVGHSERRQFF
                     HEDENFVAQKFHHVKDHGMIPILCVGETLSERENGKTEQIIAQQVLAVSAKGKDCFRD
                     CVVAYEPVWAIGTGKTATPEQAQKIHQFIRDLVGEINDSDAKHLTLIYGGSVNENNAK
                     ALFSMPDIDGGLVGGASLNAKQFVEIVKCIN"
     gene            12057..12380
                     /gene="secG"
                     /locus_tag="VAH76_000234"
     CDS             12057..12380
                     /gene="secG"
                     /locus_tag="VAH76_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO1384896.1"
                     /translation="MYQLILMIHVLIAVILIGLVLIQHGKGADIGAAFGSGASNTLFG
                     SQGTGGFLFKLTGGLAMTFFITSLMLSYMVSTQHQKAGQLAIPQQTGAPVSSVPVPVE
                     NNNKK"
     gene            12451..12535
                     /locus_tag="VAH76_000235"
     tRNA            12451..12535
                     /locus_tag="VAH76_000235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12485..12487,aa:Leu,seq:gag)
     gene            12560..12916
                     /locus_tag="VAH76_000236"
     CDS             12560..12916
                     /locus_tag="VAH76_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEO1384897.1"
                     /translation="MLSQYLPVLVFIIVALILGAVMIGAGSLLSKHSPNSAKNSPYEC
                     GFGAFESSHIPFDVRFYLVAILFIIFDLETAFFFPWALALRKIGWFGFYAMMLFLGLL
                     VIGFIYEWKRGALEWE"
     gene            13164..13640
                     /locus_tag="VAH76_000237"
     CDS             13164..13640
                     /locus_tag="VAH76_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEO1384898.1"
                     /translation="MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMH
                     VGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA"
     gene            13663..14346
                     /locus_tag="VAH76_000238"
     CDS             13663..14346
                     /locus_tag="VAH76_000238"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEO1384899.1"
                     /translation="MTKNEYLIEKLQADLANHITELTSAYGEVTIECEVQNLLPVMIE
                     LRDREEFSFDQLIDLCGVDYLHYGDYDWETESATEHGFSRGVERQEAKAYAVNKPRFA
                     VVYHLLSTQKNHRLRVKLFVEESHLIVPSVHHLWKSANWFEREAYDLYGILFDGHPDL
                     RRLLTDYGFIGHPFRKDFPLSGEVEMRYDAKLQKVIYAPVDIVPRIVVPKVIRNDNRY
                     IGNEGSKND"
     gene            14324..15592
                     /locus_tag="VAH76_000239"
     CDS             14324..15592
                     /locus_tag="VAH76_000239"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEO1384900.1"
                     /translation="MKVAKMIELKNYTLNFGPQHPAAHGVLRLVLELEGETIVRADPH
                     IGLLHRATEKLAETKPYIQSIGYMDRLDYVSMMCNEHAYVMAIEKLLGIEPPLRAKYI
                     RTMFDEVTRILNHLLWLGATALDIGAMTVFLYCFREREDLFDCYEAVSGARMHATYYR
                     PGGVARDLPDTMPQYKPSRWHNEREIEKKNHNRQGSLLDFLWAFTERFPRCVDEYETL
                     LTDNRIWKQRTVDIGIVSPENALQWGFTGPMLRGSGIAWDLRKKQSYAAYDRVDFDIP
                     VGKTGDCYDRYLVRVEELRQSNRIIRQCIEWLRKHPGPVKVDDYKVTPPRRVVMKHDM
                     EALIHHFKLFTEGFCLPRGEVYSSVEAPKGEFGIYMVSDGANKPYRLKIRAPGFAHLS
                     SFDDMVRGHMLADGVAILASQDIVFGEIDR"
     gene            15653..16156
                     /gene="nuoE"
                     /locus_tag="VAH76_000240"
     CDS             15653..16156
                     /gene="nuoE"
                     /locus_tag="VAH76_000240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEO1384901.1"
                     /translation="MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIV
                     QEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRD
                     SAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
                     KVLEQYQ"
     gene            16175..17452
                     /gene="nuoF"
                     /locus_tag="VAH76_000241"
     CDS             16175..17452
                     /gene="nuoF"
                     /locus_tag="VAH76_000241"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEO1384902.1"
                     /translation="MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQL
                     IIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLN
                     PHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
                     FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFAS
                     VPVIMEKGGEWFLKLGKPNNGGAKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIK
                     GRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALY
                     RISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICA
                     LGEAAAWPVQSFVKHFYDEFEYFIKHKRSIVAA"
     gene            17469..19820
                     /locus_tag="VAH76_000242"
     CDS             17469..19820
                     /locus_tag="VAH76_000242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEO1384903.1"
                     /translation="MATIEIDGKTFEAENGKMIIEVADEAGIYIPRFCYHKKLSVAAN
                     CRMCLVEVENGRKPVPACATPITNGMKVFTKSEQAIHSQKVVMEFLLINHPLDCPICD
                     QGGECELQDISMGFGADKSEYTESKRAVDDENLGTLISTEMTRCIHCTRCVRFGEEIA
                     GVRELGATGRGEKTQIGTYVEHSMTSEVSGNIIDLCPVGALTSKPYRFKARAWELTQH
                     DSVAPHDCLGSNVHIHTRNNKLLRVVPRENESINETWLSDRDRFSYLGLNAASRASKP
                     QIKKNGQWEAVDWETALKFAAEGISRVIKQHGPEQMAAFASSSSTLEEMYLLQKLMRE
                     LGVQNLDHRLQQIDFRDQAFLPTTPVSTLPYAEIDHQHSILLIGCNIHREVPLAGTRV
                     RKAFRNGAKIYALNPVDFDYHFDLSGRVIISPLEMPMQLAKLALALTTELASLPEEVQ
                     KLLIGLEVDKQTKQIAQSLKEDKACLITGAIVENHPEASLLRTLVAIVQKLSGAKLVR
                     LTAGANSAGACIAGMLPHRTVAGKSIAEPGLNVQEALNSKLKGYLLMGVEPGYDFANP
                     AGARQSMLAAEFVVLLSAYEHDSMHDYADVILPIAPYAETSGTYINIDNTWQTVKGAM
                     LPFGESRPAWKVLRVLGNLLHCKKFDYTSTEDILEEVKEAVSMTMEHEYEPYYPESLP
                     VINQSLVRVGEWPLYRIDAITRNAKELQLCAASESACIRIHPSTADRLKLEEIATVSQ
                     GDIEITLPLKRDERIAVDVVWVANAMPETVDLGHSFAAITIKR"
     gene            19833..20855
                     /gene="nuoH"
                     /locus_tag="VAH76_000243"
     CDS             19833..20855
                     /gene="nuoH"
                     /locus_tag="VAH76_000243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEO1384904.1"
                     /translation="MLHTIGILIWIIIKILVIVVPLLLSVAYLTYAERKVIGYIQVRI
                     GPNRVGLKGLLQPFADLLKLITKEIIVPTRSNKYLFVIAPLFALVPSLVGWAVIPFQE
                     GVVLANINAGVLYLFAMSSLGVYGVLIAGWASNSKYAMFGALRSTAQTVSYEIAMGFA
                     LVGVLLAAGSMNLTDIVNSQKGGMLHWWFIPLLPLFLVFWIAGIAETNRAPFDLAEGE
                     SEIVAGFHVEYSGIGFALFFLSEYASMILISTFMAILFMGGWLSPFEGITFLDQIFFV
                     VPGFVWLLLKISFFLFVYLWVRATFPRYRYDQLMRLGWKVLIPVTIVWLIVTSLMVVA
                     HVKPWF"
     gene            20874..21374
                     /gene="nuoI"
                     /locus_tag="VAH76_000244"
     CDS             20874..21374
                     /gene="nuoI"
                     /locus_tag="VAH76_000244"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEO1384905.1"
                     /translation="MKKVYHYIIHYVRTYLLLELLAGLWLTVKYFFRKKITVQFPEEQ
                     TPLSPRFRGLLALRRYPNGEERCIACKLCEAVCPALAITIESEQREDGSRRTTRYDID
                     MFKCINCGLCEESCPVDSIVVTPIHHYHISERGQNIMTKEKLLAVGDLMETQLAADRA
                     ADEKYR"
     gene            21386..22045
                     /locus_tag="VAH76_000245"
     CDS             21386..22045
                     /locus_tag="VAH76_000245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEO1384906.1"
                     /translation="MHEWVIQAIFYIFALLTVLSALMVISQNNPVRCVLFLVLTFFTS
                     AVLWVLAEAEFLALILILVYVGAVMTLFLFVVMMLNIDIESMKSHLIKYLPFGLIIVA
                     LLTGLLMVAIPKDLFKNSVQIQEKPLSVSSQLMDLSTQDELVPPENATSNTEKLGMVL
                     YTDYILAFEIAAVILLVAIVSAITLVHRGAIRSKRQDITQQIMTRRAERVKLISMKSE
                     K"
     gene            22063..22368
                     /gene="nuoK"
                     /locus_tag="VAH76_000246"
     CDS             22063..22368
                     /gene="nuoK"
                     /locus_tag="VAH76_000246"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEO1384907.1"
                     /translation="MIPVYDYLVLGVILFGLSLVGIMLNRKNIILLLVCVELMLLAVN
                     TNFIAFSHYYNEVGGQVFVFFILTVAAAEAAIGLAIVMLLYRNRGNIDVDKMNHLKG"
     gene            22373..24346
                     /gene="nuoL"
                     /locus_tag="VAH76_000247"
     CDS             22373..24346
                     /gene="nuoL"
                     /locus_tag="VAH76_000247"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEO1384908.1"
                     /translation="MSIQQVCLVIVLTPLVGSAIAGFFRNQIGRVGAHTVTILGVAIS
                     LLCSIFLAWGLFSGSIPNTNSLVYHWASGGALIPYEFNIGFLIDPLSVVMLVIVNFVS
                     LLVHVYSIGYMADDDGYQRFFSYISLFTFMMLMLVSANNFLQLFFGWEGVGLVSYLLI
                     GFWYQKESAIEGSLKAFLVNRVGDFGFVLGIGLIFAYAGSLDYEKVFTSANYLASQNI
                     ELFSGYSWSLITVICLLLFVGAMGKSAQVPLHVWLPESMEGPTPISALIHAATMVTAG
                     VFMIARISPLIELSTAALSTVLVIGATGALFTGILALVMNDIKRVVAYSTLSQLGYMM
                     VAMGASAYSAGMFHLLTHACFKALLFLGAGSVIIGMHHEQDMRKMGGLWNKMPITYVT
                     YVIGSLALCAFPPFAGFYSKDTIIEAAQLSQIPGSSYAYFCVTAGAMVTALYTFRSLF
                     MTFHGKPRMDEHTLSHIHESPWVVWLPLVLLAIPSIILGYVLYMPILFDTPTLLGSSI
                     FVLPEHNVLAELAHEVSSPFASAVHAIYSLPFWITVLGAIIAWVCYIAVPSIPGYLAR
                     YFSIIYSILLNKYGFDRFNELFFVKGARGLGIAFYKIGDQKLIDGAVVNGSGRLVRWF
                     SGKGRKIQSGYIYHYATVMVFGLLAFLCWLILD"
     gene            24362..25867
                     /locus_tag="VAH76_000248"
     CDS             24362..25867
                     /locus_tag="VAH76_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEO1384909.1"
                     /translation="MHYLLNLLIWLPILGGVFVLLTGDDKNPNVSRYLTLFTILLCLV
                     LCIPLVKGFDLQSFGMQFIEEIPWMPALGINYTLGIDGLSLLLIVLSIFTNLIVILAT
                     WDSISKKVSQYLSAFLIMQGLLVGIFSAMDAIVFYIFWEATLIPMYLIIGIWGSDNRV
                     YAAIKFFLYTFLGSVLMLASFLYMGYIAGSFKIETFYAIKLGMTAQTLIFIAFFLGFA
                     IKIPMFPVHTWLPDAHTEAPAGGSVVLAAILLKLGAYGFIRFSLPIVPDACSKYAPLM
                     VALSLIAVVYIGLIAIIQKDMKKLIAYSSISHMGFVTLGCFAIFAIARHGGLSNAGLI
                     LEGAIVVMISHAFVSSAMFAGVGFIYDRMHTRQLADFGGIVNTMPIFASFFMLFAMAN
                     AGLPGTSGFVGEFMVILGSIKAGFWVAFWAATTLIIGAAYTLWMYKRVIFGPVANKKV
                     AALKDISGFELSAYVLLAIMVIALGVYPKPMLEFVHQTVSHTLALADKSKL"
     gene            25921..27363
                     /gene="nuoN"
                     /locus_tag="VAH76_000249"
     CDS             25921..27363
                     /gene="nuoN"
                     /locus_tag="VAH76_000249"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEO1384910.1"
                     /translation="MMTLLENIHVAIPEMIILITACLALLSDLFFRHKLKSIAFYISC
                     VGIIVSAAVSFVFIGSYKLLIFNKLFISDDISHLMKLFIDITVLLSFVYSQNYLDERQ
                     MPTGDYYVLGLFSTLGMMILVSAHSLLSLYLGLELMSLPLYAMTAIRRTDSDASEAAM
                     KYFVMGAIASGMLLYGISLVYGATGKLDLLDVANAVAVNWQQQNTLFAFAMVFILAGV
                     GFKLAAVPFHMWAPDVYQGAPSSVTLFISTAPKIAALGMALRFLTIGLVDITMQWQQI
                     ILVMALLSTGIGNLLAVIQTRIKRLLAYSAISHIGYALFGVLAASSAGYAAALYYILV
                     YAVMSAAGFGLVVLMSNHGMEIDSIDDLKGLNKRNPWLAFMMLLVMFSMAGVPPTVGF
                     FTKLLVLKALVDVHMTWLAVVGLLFTVIGAYYYLRIVKIMYFDQPENENIVRIGTGNT
                     IVYSLNCLSLLYLGIFPGALIAACINAFAN"
     gene            27429..27505
                     /locus_tag="VAH76_000250"
     tRNA            27429..27505
                     /locus_tag="VAH76_000250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27463..27465,aa:Met,seq:cat)
     gene            27618..28061
                     /gene="rimP"
                     /locus_tag="VAH76_000251"
     CDS             27618..28061
                     /gene="rimP"
                     /locus_tag="VAH76_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO1384911.1"
                     /translation="MINDDLIVLLEPIIKNMGYELWGCEYLSQGKHSLLRIYIDKPDG
                     IGIDDCQEVSKQVSAMLDVEDPIPGHYSLEISSPGIPRPLFSIWQYQRYLGYEIHVKT
                     FKPVNGKRKLSGIIVSASEDTIVLDINNEHQEILLSNIVKANLTV"
     gene            28073..29551
                     /gene="nusA"
                     /locus_tag="VAH76_000252"
     CDS             28073..29551
                     /gene="nusA"
                     /locus_tag="VAH76_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO1384912.1"
                     /translation="MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIG
                     VRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSI
                     EFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKA
                     EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEI
                     GENIIDIKAAARDPGNRAKIAVKTNDGRIDPIGACVGMRGARVQAVSSELGGERVDII
                     LWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVAKEQLSQAIGRNGQNVRLASQLT
                     GWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHGFSSLEEVAYVPKE
                     ELLAIEEFDEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLA
                     SKGITTMEDLAEQSVDELMEIEGISEEKAGALIMKAREPWFQ"
     gene            29640..32246
                     /gene="infB"
                     /locus_tag="VAH76_000253"
     CDS             29640..32246
                     /gene="infB"
                     /locus_tag="VAH76_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO1384913.1"
                     /translation="MADVTVKQLAQVVGIPVERLLNQLQEAGLSFTDDQQTVNEEQKR
                     ILLNHLKGSSNRDISAAPERITLRRKSMSQVTVGHDMHSGKTVNIEVRKKKTFIKRSA
                     IPEQAEVEEPVVPPVVEEPVHEEITVVSDVSAETPELKETEEHPVIEPVAELDETVKE
                     EEKINLEENTAESQDELAHANTDVIENLVDVVEETIPVSKKEEVKPEKVSKKKHLEQT
                     DTDISEFKKGKKKPKYHTFEHDEEEQELHRRGGRSKFKKKKGTEKSDKYREAEETLTH
                     GFALPTAPIVREVLIPETITVAELAKRMSVKAAEVIKVMMSLGAMATINQVIDQETSV
                     IVVEEMGHKPVIIKEDAVETGLGEAISKGTKTEGRAPVVTIMGHVDHGKTSLLDYIRR
                     TKVAAGEAGGITQHIGAYHVSTPKGNITFLDTPGHAAFTAMRARGAQATDIVILIVAA
                     DDGVKPQTIEAIQHAKAAKVPIIVAINKMDKPDADPERVMNELSVQEVIPEAWGGDTM
                     FVNISAKSGMGIDDLLDAILLQSEVLELKAVTDGAAKGVVIESRLDKGRGPVATVLVQ
                     SGTLHKGDILLAGFQYGRVRALVSDNGDLVDSAGPSIPVEVLGLSAIPHAGDEAVVVP
                     DEKKAREVALFRQGRFRDVKLARRQKTTIEGIMENMTATESKVLNIVLKADVQGSLEA
                     ISDALTKLSTDEVKVEVISSGVGGITESDVHLAIASNAILIGFNVRADGTAKRLAEQE
                     SVSIHYYSVIYDIVDQIKGALTGMLAPQFKEEIIGIAEVRDVFKSPKIGAIAGCMVIE
                     GVVKRNNPIRVLRSNVVIYEGTLESLRRFKDDVLEVRQGFECGIGVKNYNDVKPGDLI
                     EVFETVEIKRDL"
     gene            32250..32621
                     /gene="rbfA"
                     /locus_tag="VAH76_000254"
     CDS             32250..32621
                     /gene="rbfA"
                     /locus_tag="VAH76_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO1384914.1"
                     /translation="MNNNFKRTDRIAEMIQRKLALIIPQEIKDPRLKGFVTISAVKVA
                     ADLGHAKIYFTVLNEDKSVVTNILNGAASYLRSALARSITLRTVPQLHFIYDESIEYG
                     QRLSRLIDEVNPPDSSSDDNN"
     gene            32608..33519
                     /gene="truB"
                     /locus_tag="VAH76_000255"
     CDS             32608..33519
                     /gene="truB"
                     /locus_tag="VAH76_000255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO1384915.1"
                     /translation="MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTG
                     SLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYT
                     VSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
                     EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDE
                     LQDMPLSQRLACLIPIDQAIQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYG
                     EQSQFIGIGQALIHGDIKAKRLVSFAL"
     gene            33667..33942
                     /gene="rpsO"
                     /locus_tag="VAH76_000256"
     CDS             33667..33942
                     /gene="rpsO"
                     /locus_tag="VAH76_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO1384916.1"
                     /translation="MSLNSAEKAEIINEYKRGDKDTGSPEVQVSLITSRIKYLTDHFK
                     ENKKDFHSRRGLQELVNKRRKLLKYLKRNDQDRYQTLIQNLGLRDSY"
     gene            34057..36246
                     /gene="pnp"
                     /locus_tag="VAH76_000257"
     CDS             34057..36246
                     /gene="pnp"
                     /locus_tag="VAH76_000257"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO1384917.1"
                     /translation="MAKITKEIVFGNHNLILETGEVARQADGAVMASMNGTQVLVTVV
                     WKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLF
                     PDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
                     LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSI
                     EELAREVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLDELREQT
                     ISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLE
                     RTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQV
                     GSPKRREIGHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDI
                     KISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRT
                     IIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKMALEEAQKQIKALIAEIEVGQTY
                     QGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQG
                     RVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSGNKISEEEE"
     gene            36381..37436
                     /locus_tag="VAH76_000258"
     CDS             36381..37436
                     /locus_tag="VAH76_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEO1384918.1"
                     /translation="MKLFSFFLVLISFASLGQGLPLHLLKCPPGFSVSIYAAPVSDAR
                     EMALGSKDIVFVGSRSAGKVYAVIPDKKKSHGTRVLTIASGLNMPNGVAFHKGALYVA
                     EIGRILRFDNIESNLNNPPEPVVVTTKLPKEKAHGWRFISFGPDDKLYIGIGAPCNVC
                     LMEDTRYATIMRMNADGQDLEIYAKGIRNTVGFDWDPVHHNLWFTDNGRDWLGDDLPP
                     DELNVAPRKGMDFGFPYCYGKSTADPVYGKTHSCSEFTPAVFEFPAHVAPLGMSFYTG
                     KLFPPNYWGQIFISEHGSWNRSHKIGYQVTTVKIKNNHVTEVKPFISGWLQGEKAWGR
                     PVDTLVMPDGSLLISDD"
     gene            complement(37531..38070)
                     /gene="folE"
                     /locus_tag="VAH76_000259"
     CDS             complement(37531..38070)
                     /gene="folE"
                     /locus_tag="VAH76_000259"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO1384919.1"
                     /translation="MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLD
                     EIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIV
                     DFYARRLQIQERLTGEIAHCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
                     LGEMRNNSSCRLEFLNLIR"
     gene            complement(38082..38423)
                     /locus_tag="VAH76_000260"
     CDS             complement(38082..38423)
                     /locus_tag="VAH76_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEO1384920.1"
                     /translation="MNCLFCKIAQGEIPATVVFEDKNILAFRDIRPQAPTHLLIIPKK
                     HIATINDVNDDDSELLANILIRAKKLAQAEGLSETGYRLVFNVNSGGGQEVYHIHLHL
                     LGGRQMTWPPG"
     gene            38553..39089
                     /gene="ppa"
                     /locus_tag="VAH76_000261"
     CDS             38553..39089
                     /gene="ppa"
                     /locus_tag="VAH76_000261"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEO1384921.1"
                     /translation="MSLMEIQSGRDVPNEVNVIIEIPMHGEPVKYEVDKKTGALFVDR
                     FMTTAMFYPTNYGYIPNTLSEDGDPVDVLVITPVPLISGAVISCRAVGMLKMTDESGV
                     DAKILAVPTTKLSKMYQSMQTYQDIPQHLLLSIEHFFKHYKDLEEGKWVKVEGWVGPD
                     AAREEITSSINRYNHTKK"
     gene            39239..40504
                     /locus_tag="VAH76_000262"
     CDS             39239..40504
                     /locus_tag="VAH76_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO1384922.1"
                     /translation="MQLPLSTLLVLLFFLICLSAFFSGSEIGMMSINRYKLRHMVKSN
                     DKQAIRVNSMLARPDKLLSVVLIGNTLANIVASTLATLIGQRFYGDAGVAIATALLTI
                     LILVFAEMTPKTLAAIYPQQVAFASSLPLSILQWIFSPLVHLISLISNGILRLFNISV
                     KRMQKEMLTGEELRSVVHEAGGLMPVEHKSMLISLLDLEQATVEDIMVPKADIVGIDL
                     DLPWIELLDQLETAQHTRLPIYHGSIDHLVGMIHVRNILNLALENKLDMDNLLNSAEV
                     PYFIPEATPLNIQILNFRKMKRRSCFVVDEYGDILGLVTMEDILEEIVGEFTTDIADL
                     SRDITPQQDGSVIVDASITIRHLNRMMGWSLPLIGPRTLSGLIIEHLGYIPPPDSCLV
                     IDHYHIEILRISDNMIRGVKMIKANKRRK"
     gene            40745..41461
                     /locus_tag="VAH76_000263"
     CDS             40745..41461
                     /locus_tag="VAH76_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO1384923.1"
                     /translation="MKILYVPFPRNQAGDLKSMVELWKKNDEQFSPDRVEIIYFNDDI
                     NYDQLDEKITIYICAHGSDDENLTKLFNHSNPLVAESIDIKEVAERFERDILPISYWI
                     SAVHLYCCGSKNKNQMMAELLGHSLLRPEKPIYHYSGSVSIVDEFGKQWSFANHVKIP
                     VHLTAKRMFVLNFFDEEQPHRASVKKEFQNKTYKELLAKKEDSFFAKVKENRASVLLS
                     KRLGKKSQDGEDNSLLRKGN"
     gene            41739..42380
                     /locus_tag="VAH76_000264"
     CDS             41739..42380
                     /locus_tag="VAH76_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384924.1"
                     /translation="MKEQFFQTKDLNTLLRSVEKPIQDLLDLNFKTLKTVSYVTPIEL
                     FNVLKPEEVLEKNMNVFIQNSQKAMSYMLNVFQIMEHHWINIYDQMAENQNEKKANPA
                     INKANRKVDTSTPKSSASKKAVTAKGKTKPAAVQSKSKTSKAKLKISSPTKVKTNTQE
                     RPAAVKQQSQMPLSSLQKDMEKQGINTPVELKNSVAILKNETKDIVSSNNKLK"
     gene            complement(42607..43332)
                     /locus_tag="VAH76_000265"
     CDS             complement(42607..43332)
                     /locus_tag="VAH76_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO1384925.1"
                     /translation="MSQRHYLLFIDNEPISEELKDYFEKFNLDIIQQKKLFPIHVEKG
                     LPTAILINWSILRTEPEAIEQFYNMYPVPLIVINNKPDEESSVAMLEAGADDFMTKPI
                     YPRELHARIGAISRRVIRAQQKVDHGKEVLMFANWRLYPASRQVFGENNEELQLSAGE
                     YDLLLTFVQQPQRVLDREFLLQITKNTDLVPFDRRIDVQISRLRQKIEIDKKKPALIK
                     TIRNGGYMFTARVVTLKESDATL"
     gene            43555..45033
                     /locus_tag="VAH76_000266"
     CDS             43555..45033
                     /locus_tag="VAH76_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384926.1"
                     /translation="MTQKTLTLPNWFVTDNDRAMARIKYELKQLETQLGKCVKQPDDT
                     AFSKNKYSLYMALADKYSEYLKLIDIDSKFKQFVGAEIPKDISYWEACTRIMHLKQKR
                     SAAVNLLEEAKIIIEISLLYQCLAKAIEPNEVSDVSNHLILNQFDKLKQLFKKENLEL
                     WLSTLVDLWNHNSTLLREPAKLFRNWDLEEQTIALGFFSESEVVNLVNAIFFYKLYPD
                     KLFNELIHPEKLVSVRMRLGFLHEFIELLQKQLNSNALQYGLNPGIDFLFHGEELPQG
                     IMIEVNEVYRGIIQLAVKNLRVQFTPENNEKVTLERLHDLGRAYKFWFNPNRLIDAVM
                     VLQQRLVTESISQEEDLEKFHQEMLVLYRQLTTTECLDLYGYFANNDSRYLLYTLYSI
                     NNGYSLDWLPSLNSSERNAIARVYQALKCVMEALRVELKNRFITTEPYLYDLGKKEIH
                     TGRRNREAVFRVIAIYGCERIVMSDSLERLFNLIEEPNQQFE"
     gene            complement(45138..47135)
                     /locus_tag="VAH76_000267"
     CDS             complement(45138..47135)
                     /locus_tag="VAH76_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEO1384927.1"
                     /translation="MKKKHLIFAYFSQFLLFNLCFFGLQLLLLYSHAESLISSIKLPL
                     PIYFEIIGTLVIQFSLYIIISMMQALLLIGILNRSWHYFSTEQWQTIIWSLFACAILS
                     ANAYYFPLSVFSKLFSPPIPEFIFLILLYFSLACLGLLLLNCLFYRTTARLLSIALPI
                     GICIFLLNNYWKSSLNPNVDSEPNVIILGIDSLSPESVTSRNMPFLHQVLLDSFQFTN
                     TISPLARTYPAWSSILTGLYPKHHLAEENLVERSRVKSQLSIVWDLNKLGHNTIYATD
                     DRRFNSIDENFGFKKIIGPKTGVNDVMLGSYNDFPLGNLLINFRISAWLFPYNFSNRA
                     SFISYYPETFTNELKRELSLQQNSPVFLAVHFALPHWPYAWAASLPDQVENEFSLSKR
                     DALYLRALKAVDNQFKIIFCYLKKHGYFKNSLVIILSDHGEVLYYPNSRLTNYQKYQS
                     SLTSKLAEYFKAKTATELDKSAGHGSDILSPMQYHSLLAFTIYKDGKPLTKTKKIKTR
                     VALFDLAPTILDFLNITKKQKMDGISLLPTVLNPNTPLPHRTFFIESGMYPNQDFTKE
                     KAIELGKKIYKVNPDTGQLELKPDELIQVNNQKLYGVISGNWILALYPDDNTYIPVIL
                     NLSTGEWIDNLQSDFAKSSPAEKLKRQLQEFYGKKLAYPIQ"
     gene            complement(47132..47992)
                     /locus_tag="VAH76_000268"
     CDS             complement(47132..47992)
                     /locus_tag="VAH76_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEO1384928.1"
                     /translation="MFKSIFILCRKKILVLLLLSLCFITSTASASFHRSLWPKWLVNA
                     PLSKQSISHQAWQHFLDHHVITNEEDINLVDYTNINEKELASLKEYIKNLSQIDIDNY
                     NRQEQLAYWINLYNALTVLTVANYYPIANIQEINISPGLFSVGPWGANIITIKNTNLS
                     LDDINNRIIRPIWNDPRTHYALNNATIGAPNLSKQAYQGPLLEQQLNDAAFKYINSLR
                     GVHVIEGKLIVSKLYDWYEEDFGGTKKYVIKHLLQFAKEPLRNQLKHINTIDSYIYNW
                     HINCPADNQS"
     gene            complement(47985..48578)
                     /gene="recR"
                     /locus_tag="VAH76_000269"
     CDS             complement(47985..48578)
                     /gene="recR"
                     /locus_tag="VAH76_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO1384929.1"
                     /translation="MDALSRLVEALRCLPGVGPKSAQRMVFHLLQHQRQRGLHLASCL
                     EQAMKHISHCQQCNNYTEQTLCTLCQNPNRDSTLLCVVESPADVSAIEQSNSFQGKYF
                     VLMGKISPLDGLGPDDIGLPKLKELIIREKIQEVILALSPSVESQTTIHFIHQLLKDE
                     TVNISQLAHGIPSGGELEFLDGNTISSALKNRAVINV"
     gene            complement(48587..48925)
                     /locus_tag="VAH76_000270"
     CDS             complement(48587..48925)
                     /locus_tag="VAH76_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO1384930.1"
                     /translation="MDINQNLGNLMKEAQKMQQRMQEAQQQLSQLVVSGESGGGMVTI
                     KMNGRHDVTEVKIKPTLMDEDIEMLEDLIAAAVNDAVRKIEKASKEKISQLTAGLNIP
                     TDLMGGKEGE"
     gene            complement(48959..50629)
                     /gene="dnaX"
                     /locus_tag="VAH76_000271"
     CDS             complement(48959..50629)
                     /gene="dnaX"
                     /locus_tag="VAH76_000271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO1384931.1"
                     /translation="MSYLALARKWRPRTFSQLVGQEHINKALINALNQQRLHHAYLFT
                     GTRGVGKTSVARILAKALNCEKGITSEPCLHCDTCVAIEQGRYIDLIEIDGASKTRVE
                     DTRDLLDNIQYAPTNGRFKIYLIDEVHMLSQHSFNALLKTLEEPPSHVKFILATTDPQ
                     KLPVTVLSRCLQFNLKHLPAELINQQLQLILQEERLDFEIQALDILAQAARGSMRDAL
                     SLLDQAITSCETKLLASDVKMLLGHTQQDYALLLLQALAKQEAPIMLVLSQQIIAEGG
                     NFQYVLDEMLNYLHQITVYQCLGDNNPLISPKAEIKELAKQFSAEDTQLFYQIGIKGR
                     EEMHLAPTLAIGFNMTMLRMYTFKPASHVTTPPLAFEQNNKTCSLIVDDNTPAQNPIG
                     IEPIPVTKATDNTVAYHQTQSLSTDDKKSANSGESMNCDWNDIIPQLKLSGLALNAIE
                     NAEFLGKKEREITLGVQKGHQSLFTPTAINRIETALAGYYKSPVKIILNSQDAVHASP
                     AQQKEKATQEKQEQAEAALHADPVFQQLKQEFSAELVKNSIVLLKDDL"
     gene            complement(50655..50751)
                     /gene="ffs"
                     /locus_tag="VAH76_000272"
     ncRNA           complement(50655..50751)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VAH76_000272"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT        15152 a         8668 c        11043 g        16070 t
ORIGIN      
        1 attattttgt tctaaattaa atataaatga taatcattct catttgcaag aggtgggagc
       61 aaataaaata ttataaattg ataatagagt ttgattttgg ataaaaagtt tctgtattct
      121 gagttaaatg ataatatttt ctacttttat tatggtaaat gagtaagtgg catgaattat
      181 agacccgata tagatggatt aagggccatc gcaattttat tggtcttaat ttatcatggg
      241 ggcctctcgc tttttccatc agggtttatc ggtgttgatg tattttttgt tatatcaggt
      301 tttctcatta ctactattat ccatgaatca ctgaataaga atgatttttc attcttcgat
      361 ttttataatc gaaggttatg gcgattacag cctgttttcg ttgctctaat cgtggtgaca
      421 accctactaa gtttattgtt ttatttgcct gatgatttaa ttgaatttag tcgtagtgcg
      481 cgtaaagcat cattgtttat ttccaacttg ttttttaata aaacaaccac cgggtatttt
      541 tcacccgata cccaccaact tcctttgcta catacttggt ctctttcaat tgagtggcaa
      601 tgttatctag ttttgccact ggtgatgtat ggcttatatc ggtttctaaa taaagggcag
      661 gttatctatg ttgtgtatgg cttagtcatt ttattttttt tgctggcttt gcactactca
      721 caggcggctc ctgcccaaac ctattatcaa ttttccagtc gtatttttga gtttttaatc
      781 ggttcttgca tcgctttagg ccctcgaatt gaattctcaa tcaataagtc tattttagtt
      841 attttgggtt catcagcatt attttttata atttataccg ctacactgga taatattctt
      901 cttggatatc ctaattggca tacacttttt gtgtgtctgg caacgggttt attgatagcg
      961 ttaggtacaa attttccttc gcttgctatt gtaaagactc tctcttttcg accattagtt
     1021 tttataggag ttttatctta ttctctttat atttggcact gggtcgtctt ttcaatttta
     1081 aggtaccaaa atatcgaaga gcggtctgct gttttattta tagtttatgg cattgttttt
     1141 gttttagctt atttatcctg gcgatatata gaaaaaccga cgaaaaaatt cagacgtatg
     1201 ccttttcgtt atacggttgt gattttattg ctgatccctg ttttattttc tcatttaagc
     1261 tctttagtta ttaaatctca ttctggatat ccacaacgat ttaatcagga gctggttagg
     1321 gtttataaac aattaaagag ttacgaaagc ccatacagag cgttatgtat agataatacc
     1381 agggcagaca atgcgtggaa atgcaaaata ggagcaaaag agcaaggtgg caggactggc
     1441 cttatgatag gtgattcttt ttccaatcat tattgggggt ttatggatgt gttagggaaa
     1501 gctgccggtg tttcaatatt ggctcaagga acctcttctt gtatttcctt accaggcata
     1561 tatctttatg attggtggca tttcaaagat aaaatatatc aggattgtta tgagcaaacg
     1621 gaaaaatatt atcgcatgat ccaagagaat cattatgatt atgtgattat aggccaagta
     1681 tggaacaatt atgtttcagc ccatatcatc aatcaacctg gtgatgatta ttctgcagaa
     1741 ttaaccaaaa agcgtttggc agatgccttg gacagagcac ttggtgtgat aatatcttct
     1801 ggagcgaaac ctgtattaat cgaatcaacc gcgttaacgc atggaaactt gcatcaatgc
     1861 ttttttaagc atattaaatt aagacaaagt tataattcag aagagtgtcg ctttacttta
     1921 accagttcag ataatgagat ttggctaaac caactgtttg ataacatgcg aagaaaatac
     1981 cctcaattgg taataataga tcctaaacaa gtccaatgtc gcgataatgt ttgttattcc
     2041 gatttgaatg ggattccagt atatcgcgat gaagggcata ttactgatta cgcttcatac
     2101 caattgggtt atttatattt gcgggagttt gaaaatcctt tgatttaaaa aggttggttt
     2161 gctttgattc cccaggctac aaaacgattt tacaaatttc attcctcatt tttacgatac
     2221 agaaccagtg tatttcctat catctgaact aattcggcat gcaattgttc acaaagctcc
     2281 tcggccattt gcattctgtc ttccttttca gccccattaa ttttgacttt aattaattca
     2341 tgtgctagca aagctacatt tgtttctgcc aatatagctt cagttaagcc tttggcgcca
     2401 aggagcacaa cgggctttaa atgatgagcc ttggctttaa gtgattgttt aaaagacgta
     2461 tccactgcga tatcccagat aataaaatgg taaattattg cacgttttgc tgggaaaagg
     2521 tagtaaaatt ctgtatcata ggaaaaattg ttttatcttt attcgttatg agtcgtacta
     2581 aaagcagtaa acgttggttg caagaacatt ttgatgacgt ttatgtaaaa aaagcgcagg
     2641 ctgaaggata tcgaagtcgt gccgtatata aacttaaaga aatagatgac aaagagtcgc
     2701 tcattaaacc aggcatgaca gtggttgatt tgggagctgc tcctggcggc tggacccaat
     2761 acgcttcaga aaagatgaaa ggaagcggtc gtcttgttgc attagatatt ttacccatgg
     2821 atgctttgcc taatgtggag tttatccttg gagattttcg tgaagataat gtcttgcaag
     2881 aattaattaa tttgattcct caaaggactt tagacttgtt attatcggat atggccccaa
     2941 atatgagtgg aagctcagca atagatattc caagagccat gtatctggta gagctggctt
     3001 ttgatttcgc ggaaaaaatg ttaaaacccg gtggtaatat gctagttaaa atttttcatg
     3061 gctcgggttt tgatgaatta gtcaaacagg cacgagcatc ttttgaaaaa gtggtaatcc
     3121 gtaaaccatc cgcatcaaga tctcgttcaa aagaaaccta tttgctggca aagggctata
     3181 atttatagta gttttatata tatttataaa tatgcttagc gttgttttgg atgatatgag
     3241 ctgtgcaaga gctattttgg gtctcgcaac aaaattgtaa gtaactaacg aggttaatac
     3301 attgaacgac atggttaaaa atttattttt gtggctgata atagcgatag ttcttgtctc
     3361 tgttttcagt aattttggtc ctcgtaactc agttgctgag aaaatttctt atagccagtt
     3421 tttacaggaa gtggatcaag ggatggttaa ctctgttacg attgaggata acaaaatcat
     3481 caagggcgta accaaaaata ataaacgctt tgtcacgtac atgccgatgc aagacaatgc
     3541 tttgttaggc gaattgttga aaagcaaagt cgatgtcagt ggtcaggaaa agcaacagga
     3601 aagtttcctt ttgcatttat ttattaactg gttcccaatg ttgttattga taggtgtatg
     3661 gatatttttc atgcgccaaa tgcaaggcgg cggtggtcgc ggcgcaatgt ctttcggcag
     3721 atcaagagct cggctattag gtgaagatca ggttaaagtc acctttgcag atgttgcagg
     3781 tgttgatgaa gccaaggaag aagtgaaaga gcttgtcgat tttttacgtg atccaacaaa
     3841 gtttcaaaat ttgggtggac gcattccccg cggtgttttg ctggtcggct cccctggtac
     3901 aggtaaaaca ttattagcca gggcagttgc cggggaagcg aaagtccctt tctttaccat
     3961 ttcaggttct gattttgttg agatgtttgt tggggtcggt gcgtcccgag tgcgtgatat
     4021 gtttgagcaa gccaaaaagc atgcgccatg tatcatcttt atagatgaaa tcgatgcagt
     4081 aggtagacac agaggcgccg gcttgggtgg cggtcatgat gaacgcgagc aaacacttaa
     4141 ccaattgctt gttgaaatgg atggatttga aggtaatgaa ggagttattg tcgtagcggc
     4201 aactaaccgt cccgatgttt tggaccccgc gcttttacgt cccggccgct ttgatcgtca
     4261 agtcgttgtt cctttgcctg atatcagagg tcgtgagcaa atattaaaag ttcatttaca
     4321 aaaggtccct gtagatagcc atgtagaagt taaagctatt gcacgcggca ccccaggttt
     4381 ttcaggagcg gatttagcca atcttgttaa tgaagcggca ttatttgctg ccagggctaa
     4441 taaacgcaaa gtgggcatga ttgaactgga taaagcaaaa gataaaatta tgatgggggc
     4501 tgaaagacgc tccatggtaa tggatgataa cgagaaaaaa ctcaccgcat atcatgaagc
     4561 aggtcatgct atcgttggtc tgtcagttcc tgagcatgat ccagtttata aagtatcaat
     4621 aattcctcga ggccgagctt tgggcgtaac tatgttttta ccagaacaag acagatatag
     4681 tcacagcaaa cgccgtttgg aaagtcaatt atgcagtctg tttggtggtc gaattgcaga
     4741 ggaattaatt tttggtcctg aaagtgtgac aacgggtgcc tccaatgaca ttatgcggtc
     4801 tactgagatt gctcgcaaaa tggttacaac ttggggctta tctgctttag gtcctctaac
     4861 ttttggtgag gaagaggaag aaatattttt aggacgctca gtgaataaac ataaggagat
     4921 gtccgataga acagcacagc aaattgatga tgaggttcgt gctattatcg acagaaacta
     4981 tcaaagagca aaagaaatat tggaaaccaa tatcgataaa ttacatttaa tggcacaaag
     5041 ccttatcaaa tacgaaacga tagacaccaa tcaaattcaa gaaattatgt ctgggaaaga
     5101 acctacccct ccagaggatt gggggtcaac aaaaccgatg gataaggctg agtcagttaa
     5161 tgatgctcct gcaaaaccta ttaatggtga aacaatagaa aatccggcag aagggcatta
     5221 attttatcag tagctattta tcgagagtac cctgtgaatt ctgaacagtt cctcggttgg
     5281 cttgagcgaa agagccaacc tgcttcttcc ttggtttttg aaaagccgtt aattatgggt
     5341 gtgctaaatg ttacctctga ttcattttat gatggtggca agtatctgtc tgtggataga
     5401 gcttatgagc aagccataca attaattgca tgtggagctg atctcattga tattggtggg
     5461 gaatcaacca aacccggtgc tccgccagtc cctttagatg ttgaactatc aagagttctt
     5521 cccataatca aacaacttcg tgcgttcaca gatgtgtgta tttctattga tacaaataag
     5581 ccagaagtga tggaagcggc aattaatgct ggtgcgaatg ttataaatga tgtttatgct
     5641 ttaagaaggg atggtgctct gaatgttgct gcacaattag atgttcctgt ctgtttaatg
     5701 cacatgaaag gagagccgcg aaccatgcaa gaaaatcctt cttacccaga agggataatg
     5761 aaggaattac agcatttttt tgaagaacgc atcgataaat gccagaaagc cgggctgaat
     5821 aggaataaat taattcttga ccctggcttt ggttttggaa agcgcgttca agataaccta
     5881 caattaattt atcatctgaa cgaattgcat tcttttggat tgccaattct tttgggcgtt
     5941 tcacgcaaaa gcacaattgg tgccgtgttg aatactgagg ttgaacaacg tttgattgga
     6001 agtattacgc tagggatata tgcggctctt aaaggcactg caattatcag gacacatgat
     6061 gtagatgaaa cgaatcaagc attaactatg attaatgcaa tttatcaagc tgctaaagaa
     6121 atcagttaat agctcatgag atgatcagaa gatgactgag actggaaaaa ttaaataata
     6181 aggtaggtaa ggatgagtca acgtaaatat tttggtacgg atggaatacg gggtcacgtt
     6241 ggattatcaa atattaaccc tgaatttgtt ttaaaattag gctgggcagt aggctgcgtt
     6301 cttgcaaacg gagcgagaaa aaaggtagtg attggtaagg acaccagagt atctggctat
     6361 atgttggaat cggctttgga ggcaggattg tccgccgctg gggttgatgt ggcattatta
     6421 ggaccaatgc ccactcctgg aatagcctat ctaacacaaa cattgagggc taatgcaggc
     6481 atcgtgatta gcgcttctca taatttattt gaagacaatg gcattaagtt tttttcagct
     6541 gatggaggga aacttccgga tagtgtagaa cttgcaattg aagctcaatt agaaaaacag
     6601 ttgcaaactg taccttcagt caagttgggt aaagcaacac gtattaatga tgcagcgggt
     6661 cgttacatag agttttgtaa atctacgatt ccttccctat ctcgactatc caatttgaaa
     6721 atagttgtgg attgcgccaa tggtgcaacc tatcatatcg ccccaaatgt attctctgaa
     6781 ttgggtgctg atgtagttcc tataggaatt aaaccagatg gttttaatat caatcaagaa
     6841 tgtggttcga cagcgcctga attattaaga gaaaaggtga ttgctgttgg ggctgatatt
     6901 ggtattggtt tggatggaga cggggatcga gttattttag tggactctct gggaaacctc
     6961 gtagatggcg atcaaattat ttatatcatt gccaaggaca gacaccaacg aggagtactg
     7021 catggagggg tcgtaggcac gttgatgagt aattatggtc ttgaattagc aattacctca
     7081 ttgggggttc cttttcaacg ctcaaaagta ggtgacaggt atgttcttga aacgctaaga
     7141 gagaaagatt ggaagattgg aggtgaaact tctggacata ttgtttgttt ggataaaaca
     7201 acaacaggtg atggaattgt agcggcactg caagttcttt caattatggt gaagcaaaat
     7261 aaagcactgc atgagttaac tgcaggtatt caattgttac ctcaaacttt agtgaattta
     7321 aaaacgaata atgctgcctt gttagcctct aatcctgatg taattcaggc ggttaaaaat
     7381 ttggaaaaac atcttaatgg agagggtcga gtgttactca gaccttctgg cactgagcct
     7441 ttactgagag ttatggtgga aggtgctaat gcatcgatag tcaagcaaca agcacaaatg
     7501 ttatgtgatg acatcagtca gatcgataaa aaattgactg agagcctacc gtccacttga
     7561 gcatctgtct ttttaagttc taatgcaggg gcttttccaa aagcccctct gtttcacagt
     7621 attaaggtat taactacttg ttggagccat ggagtggagc attgtatcag cctcttcaag
     7681 taatcggtga gatcttgttt tgaaaaaaca aggtcttttt gaatactcat attcatcttg
     7741 ccctgttaac ttacctatta acattcggaa aaaatcttca atatattccc atgcttttgt
     7801 aaatcctcta tgccgtttta aaggctcata tcgaggatct tgagtaatga gaggcatggc
     7861 ttctcggttt gaagtttttg catcagggag ttccatcaaa tgtttaattg aaataagtga
     7921 atggattgcc tgaataaata attcagcttc caatggatat tcctttttca attttacata
     7981 ttgctgatga aatttttgca attcttcagg ccaattcttt aactgctgtt cttcatcaat
     8041 cgattcatca agtataggca gagcaggttt taagcggaga atattttgta tatcttgcaa
     8101 tgggtttggg cacatgcgct tgtaagccag taatgcagat aaatcatttt gattttttaa
     8161 ttcaaacaag atgtctttta aaagatgaag tgctttaaga cccaaagtct catttacaca
     8221 agaactttgc actgcatcat ataaaccttt ataccatagc tgtatttctt ctgaatgatg
     8281 ctttatatat tccatgtttt gtacatgaaa tgaatctaat ttttgcttaa gcaggataga
     8341 agatggtggt agtaaagggg agatgctagg agcggattcc tggtgttgag gatttgataa
     8401 tttatctaac tcatggcctg tagaaaccgg tttttcttta aatgctttat caatacctaa
     8461 ggaatttact tttcctttgt ctatggcaag tgtctcagaa tcttcttcag atgagatact
     8521 agcctcatca aaaaagtcta aaatattatc ttctgataat tcatcgtgac cattgaataa
     8581 tgacaagcca tttggtttcc tggatataac tggaatctcg gggcttgatg gtctcaatgg
     8641 ttccgatggg cttgatttga caaaagccag tgtttcttgc ccgttgggta cttgttgttc
     8701 taaactggac tcagagtctt gccgaaccac ttgggtgcgg ggcgttaatt ccggaatgac
     8761 aggtatttct tgttctatct ctttatgctg tttttcacca gaaagcccag tgtctttctg
     8821 aaccatagga gagggtcgtg ttgattcaag gacgattggt gtttcttgtc tctcagttgc
     8881 ttgctgtttt tctacagtag ccacggaatc cttttcaacc atagaagagg gtcgtattaa
     8941 ttccagtgcg actggtatct cttgctctac ttctacatgc tgttttttac caacagattc
     9001 aggctcagga tgaatgattt gaggagagcg tgttgactcg agaacaagcg gtatttcgtg
     9061 tccattgcct acttgcagtt tttcagtggt agattcaagt tccttatgaa tgacttgagg
     9121 agggcatgtt gaatccagaa caactggcat ttcgtgtcca ttgtctactt gcagtttttg
     9181 ggtgatagat tcagactcct tatgaatgac tgggcggggg cgtaatgatt ccagaacaac
     9241 cggcgtttct tcctcattgt ctagctgctg tttttctatg tgagaatcat tgcctatctg
     9301 aattacttgg gcagggttca tcaattccac agtaagaggg attgctttat ctactgctat
     9361 ttcctgtttt tccaatggag gatcaatttt ttcatcgttc agattaggca aaggtaattg
     9421 atttaattca gacacaaggg attggatttg attggcaaga gtagataaat cttctttaat
     9481 agagtttatg tcttcgttct ttagcggttg atttgaaatt aaacgcaatt gctcaataag
     9541 tgcttttgct tcattggttg tttgtccatg atctttcata agcaaatcaa tttgcttggc
     9601 tgtttcaaga taagattggc atagtttaat ctggcttttt aactcttctt taagaggatc
     9661 ctcttcattc aatgaatctt tattttcttc agcttcatgc aaacttggaa gttgtacatc
     9721 catagttata ggtaaatcgt atccacttct tagttgttgt atttgtttgc ccagagcagg
     9781 catttcttga agtttttcca acgcttgtga aaattgttcc ttcttttccg aaatttttgg
     9841 catgagaatg agaagtacca aataaaaatc ggccggtgaa gagtcataag aaaggccact
     9901 tggtaaaaca aaatttggta tactttctat tggtatttca tttatcaggt caatggcatc
     9961 aggaactaat tttttataaa tagatatatc ctcttcattt gaaggaattt ctggtatcgc
    10021 ttcaatcaac agttcttgct gtttcacaag ttcatgatgt ttgattttta gctctttttg
    10081 ttgagctaat aattgcagga agctaactga attcttaagt ttttgctgat tatcagaata
    10141 attagaatcc gaataccatg aaagccagga agatgggtta aaattagata ataatccatc
    10201 aatctgattt tggtattcct gtatcaatcc ttgagggtct tcactttcct gaaatttcct
    10261 gattaactct gcttttaaat cgggtgccag ggagtggtta gtcactgcgg tttcaatacg
    10321 acccaggtct ttaagcaata gttcaatttg tttttctagc tcagtctctt ttttgttaat
    10381 atcagttgca gataattgtt tggcgaatcg cttttgcaat tcatcccatt tttcgcaagc
    10441 gcgagttaat tttttaattt ccaattcaac tgcagctata aatttttgta aactatcaac
    10501 agcatcttct tccacagggg gttgaatgcc caactgagac aaatctgtca ttaaggcatt
    10561 ccatttattt ttgagcaaaa ccaactttaa tttggcacgt gtagtttcta tttccttact
    10621 ggttttgaag agataactgt agtgcccgac tacaattgat ttttgaacct ctccgtattc
    10681 agggaattta taaggaggcg ctaatggatt ttgtttggac aggacttcgt tcgctttagg
    10741 ttcgatttct ttgtataaag gatttaattc attttttgcg tgttcaattt ttttagccaa
    10801 atcagaaatt tttctttgca gttcttctcc ttttttcatg acctcatcta gtttgtgttt
    10861 tttcctttta tactcttctt gttcttcagc taaactggaa tattcatcaa catatttttt
    10921 aattaacaat aaaagattta attgggattc aacttcaaca ggagcttttc cttccggcag
    10981 tagggttgaa ctaaatattt gctttaaatt taaggggtta attggtggag tcaaggcaga
    11041 gatttcattc attaagtgca gtatgatttc gttcattata aaaccctcct ggttaaatca
    11101 tagtaggatt ctatcgaaat caaaccacta agcaagtaga aaaatgtatt ttttgcaaaa
    11161 aaaatgaata attaattctg aaaaaaaaca tagataaagc tacacatctg ggcgaagttt
    11221 cggtatattg cacagctgca cactatcagt tgtttactga taggctggaa ataatagaaa
    11281 ggattttgtc cggctatact agaaattgtt ggagggagtg tgaggcaaaa gatagttgct
    11341 ggtaactgga aaatgaatgg ccagattcaa caggtgactg aattggtttc gcaaattgag
    11401 gaattaattg gctttgattg cgcagcacag gttgctgtaa tgcctcccag catttatata
    11461 cccaaggtaa gagactgctt aaggacgggg aggattgtag taggtgcgca aaatgtttat
    11521 ccgaaagatt atggtgccta cacaggtgaa ctgtcagctc ccatgcttaa agattttgat
    11581 tgccgatatg ttctggtggg gcactcggag cgcaggcaat tttttcatga agatgaaaat
    11641 tttgtggcgc aaaaattcca ccatgtcaaa gatcatggta tgatacctat tctttgtgtt
    11701 ggtgaaaccc tttctgaaag agagaatgga aaaacagagc agattattgc tcagcaggtg
    11761 ctcgcagtga gtgcaaaagg gaaagattgt tttcgtgatt gcgtagtggc ttatgagcct
    11821 gtatgggcaa ttgggacagg aaaaactgct acacctgaac aggcacaaaa aatacaccag
    11881 tttattagag atctggttgg agaaataaat gatagcgatg cgaaacattt gacgctcata
    11941 tatggtggca gtgttaatga aaataatgca aaagccttat tttccatgcc agatattgat
    12001 ggagggttag tgggtggagc atcgttgaat gcaaaacaat ttgtggaaat tgtgaaatgt
    12061 atcaattgat attaatgatt catgtcttaa ttgctgtaat tttaataggc ttggttctta
    12121 tccaacatgg taagggtgct gatataggtg ctgcttttgg ttctggcgca tcaaacacgt
    12181 tatttggaag ccaaggtact ggtggtttct tgtttaagtt aactggtggt ctagcaatga
    12241 ctttttttat taccagttta atgctgtctt atatggtttc tacgcaacac caaaaagcgg
    12301 gacagttagc tattcctcaa caaaccggtg ctccagtcag ttcagtccca gtaccagttg
    12361 aaaataataa taagaagtaa ctaagaagat gtagtgtctt tagggctgtt gtcttttgtg
    12421 atgaactcag gataacaaaa ataaaaataa gccgaagtgg tgaaattggt agacacgccg
    12481 tcttgagggg gcggtggcgc aagccgtgcc ggttcgagtc cggccttcgg caccatttta
    12541 aatcgagcaa ggtgaactga tgctatctca atatctaccc gttctcgttt ttataattgt
    12601 tgccttgatt ttaggtgcag taatgatagg cgctggttct ttgctttcca agcatagtcc
    12661 gaatagcgct aaaaactccc cctatgagtg tggttttggc gcctttgaaa gctcccatat
    12721 tccgtttgat gtacggtttt atctggtggc tattttattt attatttttg atctggaaac
    12781 agcattcttt ttcccttggg ccttggcatt acgtaaaata ggatggtttg gcttttatgc
    12841 tatgatgtta tttcttgggc tgttggttat aggttttatt tatgaatgga aaagaggcgc
    12901 cctggagtgg gaataaggtt ctttcattct tttaaaatat ttgatttaat cttccgtttt
    12961 agccattact atgggaagca aaggacgctt ggcaaatcat tacagagctt tttgcttttt
    13021 gaggaacccg gtagatttta ggttggtttt taaatcctgg tcgtcttttg ccaaaacgat
    13081 agctgagatt atattttaag ccattgtaat aatggttatg taaaaactgg attattaatt
    13141 atttaactgc ttagaggcga actatggcgg ttgcagaact agaaaaaaaa ggttttgagt
    13201 tgactacggt cgaaaaattg gttggttggg cacgcagtgg ctccatgtgg ccaatgacgt
    13261 ttggcttagc ttgctgtgcc gtggaaatga tgcacgtggg agcagcacgc tatgatttgg
    13321 acaggtttgg tattattttc cgtcccagtc cacgccaatc agatgtcatg attgtagcag
    13381 ggactttatg taacaaaatg gccccggcac tcagaaaagt ttatgatcag atgccagaac
    13441 cacgctgggt tatttcgatg gggtcttgtg ccaatggtgg gggatattat cattattcct
    13501 attcagtggt tcgtggctgc gatcgtattg tgccagtcga tgtctatgtt cctggctgtc
    13561 caccaacagc agaagcgttg ttatatggca ttattcaact acagaataaa atcaggcgga
    13621 aacctgtttt agaggcataa gctcattaag ggtgcagatt agatgacaaa aaatgagtat
    13681 ttgattgaaa agctacaagc tgatttagca aatcatatca ccgagttgac ctccgcatat
    13741 ggtgaagtaa ctattgagtg tgaggtgcaa aatttattgc ctgttatgat tgagttgaga
    13801 gacagagagg aattttcttt cgatcagtta attgatctat gtggcgtaga ttatttgcat
    13861 tatggtgatt atgattggga aacagagtct gcgacagagc atggattttc acgaggtgtt
    13921 gaacgccaag aagctaaggc ttatgctgtt aataaaccac gattcgccgt agtctatcat
    13981 ttattatcaa cccaaaagaa tcatcgttta cgagtcaaat tatttgtaga agaatctcat
    14041 ttaattgttc catcagtaca tcatttatgg aagtctgcta actggtttga acgagaagct
    14101 tatgatttat atgggatttt atttgatggt catcctgatt taagacggtt actgacggat
    14161 tatggtttta taggccaccc tttccgcaag gattttccac tcagtggaga ggtggaaatg
    14221 agatatgatg caaaactgca aaaggtaata tatgcccctg tagacattgt gccaagaatt
    14281 gtagttccaa aagttattcg taatgataac cgctacatag gtaatgaagg tagcaaaaat
    14341 gattgaactt aaaaattata ccttgaattt tggcccgcaa catcctgctg cacatggtgt
    14401 tttaagattg gttcttgagt tagagggtga aactatcgtt cgtgctgatc cacatattgg
    14461 attgcttcat cgtgcaacag aaaaattggc tgagaccaag ccttacattc aaagcattgg
    14521 ttacatggac agattagatt acgtctcaat gatgtgtaac gaacatgctt atgtcatggc
    14581 gattgagaag ttattgggta tagaaccacc tttgcgagct aagtacatac gaactatgtt
    14641 tgatgaggtg actcgaattc tgaaccatct cttgtggtta ggagctactg cattggatat
    14701 tggtgcaatg actgtgtttc tttattgctt cagagagcga gaagatttgt ttgattgcta
    14761 cgaggcagtt tcaggagcac gtatgcatgc tacctactac agaccaggag gggtagcaag
    14821 ggatttgcca gatactatgc ctcaatacaa accgtcaaga tggcataacg aaagagagat
    14881 tgaaaaaaag aatcacaatc gtcagggtag ccttttggat tttctttggg cctttactga
    14941 gcgatttccc cgctgtgtgg atgaatacga aacattgtta accgataatc gtatatggaa
    15001 acaacgtact gtagatattg gtatcgtttc acctgaaaat gcgctgcaat gggggtttac
    15061 cggaccaatg ttaagaggct ccggtattgc ctgggattta cgcaagaaac agagctatgc
    15121 tgcttacgat agggttgatt ttgatattcc cgttggcaag acaggggatt gttatgatcg
    15181 atatttggta agagtcgagg aattaagaca atccaatcga attataagac aatgtattga
    15241 gtggttgcgc aagcatcctg gtccagtaaa agtcgatgat tataaagtga cgcctccacg
    15301 ccgtgtagta atgaaacatg acatggaagc attaattcat cactttaaat tatttaccga
    15361 aggcttttgt ttaccgcgag gtgaagttta ttcatctgtt gaagcgccaa agggcgagtt
    15421 tgggatctat atggtgtcag atggagctaa taaaccttac cgtctgaaaa taagagctcc
    15481 aggatttgct catttatcaa gctttgatga tatggttaga ggtcacatgc tagctgatgg
    15541 agtggctata ttggcaagtc aagatatagt gtttggagag attgatcgtt aatttaacga
    15601 catgcttgct taaagtgtat taagagataa taaacgatat aaaggtttcg aaatgtctgc
    15661 taattcagca aaaatattaa atcaattgat gccggcaaac cgtatgcgcg atattgatca
    15721 ttggatatca aaatatccca aagatcaaaa acaatccgcg gtcatgagtg ctcttcgaat
    15781 agtacaagaa gaacatggtc atttaacaac ggaattaatg aatgcagtag ctgaatatct
    15841 tgatatgccg cccatcgcag tttatgaagt agcaagtttt tatacaatgt atgaacacaa
    15901 gccagtgggg cgacatttaa ttaatgtttg taccaatatt tcttgcatgc tgagggattc
    15961 tgctggcgtt gttgaacatt tagaaaaaaa attgggtgtt aatctaggtg gaacaacaga
    16021 agatggtcga tttactttaa gatcagttga atgcttgggt gcttgtgtga atgcgccaat
    16081 gatgcaagtt gataaagatt atcacgaaaa tttgactgct gaatctatag ataaagtatt
    16141 ggaacagtac caatagaaaa taaaggtgtt tgccatggtt gaactaaatc aagtttgtta
    16201 ccgaacattt catttaccag agccctggtc gctatcaact tatgaaagta ttggtggcta
    16261 tagcgcatgg cgaaaaatat taaaagagca aacatcacct cagttaataa ttgaagagtt
    16321 gaaaacttct gccttgcgcg gacgaggagg ggctggattc ccaaccggtt taaaatggag
    16381 ttttatgaat agaaactccc ctgttcaaaa atacgtagta tgtaattctg atgaaggtga
    16441 accgggtact tgcaaggaca gagagattct tactttaaat cctcaccaat taatcgaagg
    16501 aatggccata gctgggtatg ttatgggcgc cacggttggt tacaactaca taagaggaga
    16561 attctggctg ccatttgagc gtactgaggc tgcaataaag gaagcatacg atgctggctt
    16621 gctaggtaaa aatatattag gctccggcgt tgattttgat ttatataatc atttaggagc
    16681 gggagcttac atttgcggtg aagaaactgc attgcttaac tcgattgaag ggaaaaaggg
    16741 tatgcctcgt tttaaaccac cattcccggc aaattatggc ttgtatggta aaccgacaac
    16801 cataaataac actgaaacat ttgcgtctgt tccggtaatt atggaaaaag gtggagaatg
    16861 gtttttaaaa ttgggtaaac cgaacaatgg cggtgcaaag tgctttagtg ttagcggcca
    16921 tgttaacaaa cctggtaact atgaaatccc cttgggcacg ccattcaaaa cgctgcttga
    16981 tctggctggc ggcgtcatta aaggccgaaa aatcaaagcc gtgattcctg gtggttcctc
    17041 tatgccagtg ctcccaggtg acgtcatgct ggggctggat atggattatg acagtataca
    17101 gaaagccggc tcaggcttgg gttcaggcgc agtcattatc atggatgaaa ccacctgcat
    17161 ggtggatgct ttgtatcgta tttctgagtt ctacatggat gaatcctgtg gacaatgcac
    17221 accttgtcga gaaggcacag gctggttagt caggttatta cacagaatta taaacggcca
    17281 tggcgagccg ggagacgtgg agaagttagt gaatgtggcg aataacatag agggaagaac
    17341 catttgtgca ttaggcgagg cggcagcttg gccagtacag agttttgtta aacatttcta
    17401 tgatgaattt gaatatttca taaagcataa acgctcgatc gtcgcagcat aattaagaag
    17461 gttagaccat ggctactatt gaaatcgacg gtaaaacatt tgaagcagaa aacggtaaaa
    17521 tgattataga ggttgctgat gaagcgggca tctatattcc acgtttttgc tatcataaaa
    17581 aattatctgt ggcagctaat tgccgcatgt gtttggtcga agtggaaaat gggagaaaac
    17641 cggttcctgc ttgtgctaca cctataacca atgggatgaa agtatttaca aagtcagagc
    17701 aggctataca ctcccaaaag gtagtgatgg aatttctctt gataaatcat cctttggatt
    17761 gccctatctg tgatcagggt ggagaatgtg agcttcaaga tatttctatg gggtttggcg
    17821 ctgataaatc agagtacaca gaaagcaagc gtgcggtaga cgatgagaac ttaggaacgc
    17881 taatatctac tgaaatgaca cgttgtatcc attgtacacg ctgtgtgcgc ttcggcgagg
    17941 aaattgcggg ggttagagag ctgggagcta ccggccgtgg cgaaaagacg cagattggta
    18001 cttatgtaga acacagtatg acatcggaag tttcagggaa tatcatagac ttgtgccctg
    18061 ttggtgcctt aacttccaag ccttatcggt ttaaagcccg ggcatgggaa ttgactcaac
    18121 acgacagtgt tgcgcctcat gattgcttgg ggtcaaatgt tcacattcat acaagaaata
    18181 ataaattatt gcgtgtagtt cccagggaaa acgaatctat caacgaaacc tggctttcag
    18241 acagagacag atttagctat ttgggtttaa atgcggcttc aagggcgagc aaaccacaaa
    18301 tcaaaaaaaa tggccaatgg gaagcggttg attgggaaac tgcgcttaag tttgccgcag
    18361 aagggataag ccgagttatc aaacaacatg gcccagagca aatggccgct tttgcgtcca
    18421 gttcttctac cttggaagaa atgtatttac tacaaaaatt aatgagagag ctgggtgttc
    18481 aaaatctaga tcaccggtta caacaaattg attttagaga tcaggcattt ttacctacta
    18541 cgccagtaag cactttgcct tacgcagaga tagatcatca gcattcaatt ctgttaatag
    18601 gatgcaatat tcatagagaa gtaccgttgg caggaacaag ggtaagaaaa gcttttcgca
    18661 atggcgcaaa aatttatgcc cttaatccag tagattttga ttatcatttt gatttgtccg
    18721 gacgagtcat catttctcct ctggaaatgc cgatgcaatt agctaaatta gccttagctc
    18781 ttacaactga attagcatcg cttccagaag aagttcaaaa attgttaata ggtttggaag
    18841 tagataaaca aaccaaacaa atagcacaat cattaaaaga agataaagcc tgtttgataa
    18901 ccggagcaat agttgaaaat catccagaag cctcgctatt gcgtactttg gtcgctattg
    18961 ttcaaaagct gagcggagcc aagttagtca gattaacggc tggagcaaac agcgcgggcg
    19021 catgcattgc aggtatgctg ccgcatagaa cagttgctgg gaaatcaatt gcagagccag
    19081 gtctcaatgt gcaagaagcg ttaaattcca aattaaaagg ttatttactc atgggtgttg
    19141 aaccaggcta tgattttgca aatccagccg gtgcaaggca atcaatgtta gctgcagagt
    19201 ttgttgttct cttatcagcc tatgagcatg actccatgca tgattatgct gatgttattt
    19261 taccgatcgc tccttatgct gaaacatctg gaacatacat taacatcgac aatacctggc
    19321 aaacagtgaa aggagccatg ctgccatttg gcgaatcacg gccagcctgg aaggtgcttc
    19381 gtgttttggg taatttattg cactgcaaaa aatttgatta tacctcaaca gaggacatct
    19441 tggaggaagt caaagaagca gtatcgatga caatggagca tgaatacgaa ccttattatc
    19501 ctgaatcatt gcctgtcatt aatcaatcac tggtaagagt tggagaatgg cctctttatc
    19561 gaatagatgc tattactaga aatgcaaaag agttacaatt atgtgccgca tccgaatctg
    19621 cctgtataag aattcatcca tcaactgcag atagattgaa attagaagaa attgctactg
    19681 tatctcaagg agatattgag ataacattgc cgctaaaacg tgatgagcgc attgcggttg
    19741 atgttgtgtg ggtggcaaat gcaatgccag aaacagttga cctcggtcac tcgtttgctg
    19801 caataacaat aaagcgctag aaaggtaaaa aaatgcttca tactattggc atactaattt
    19861 ggattattat taaaattctt gtgatagttg tccctttact gctttccgtg gcctacctaa
    19921 cctatgcgga gcgtaaagtg attggctata ttcaagtgcg tatcggcccg aacagggttg
    19981 gtttaaaggg cttgctgcag ccatttgctg atttattgaa attaattact aaagaaatca
    20041 tagttcccac tcgatcaaat aaataccttt ttgttattgc tccacttttt gctttagttc
    20101 cttccttagt cggttgggcg gtaatccctt ttcaggaagg tgttgttctg gcaaatatta
    20161 acgcaggggt cttatactta tttgccatga gttctttggg tgtatatggt gttcttattg
    20221 cagggtgggc atcgaattcc aaatatgcca tgtttggggc gctgcgtagt actgctcaaa
    20281 cagtctctta cgaaattgca atgggatttg ctcttgtcgg ggtactcctg gccgctggaa
    20341 gcatgaactt gactgatatc gttaattccc aaaaaggcgg aatgctgcat tggtggttta
    20401 ttcccttact tcccctattc ttggttttct ggatcgccgg tattgcagaa actaacagag
    20461 ctccttttga cttggcagaa ggtgaatcag aaattgttgc tggtttccat gtagaatatt
    20521 ctggtatagg gttcgcctta ttctttctct ctgaatacgc cagtatgatt ttgatttcaa
    20581 catttatggc tattttattc atgggcggat ggttgtcacc ttttgaaggc attacgttcc
    20641 tggatcaaat cttttttgtg gtacccggat ttgtttggtt acttctgaaa attagtttct
    20701 tcctttttgt gtatttgtgg gtaagggcta ctttcccccg atatcgttat gaccagctta
    20761 tgcgtttagg ctggaaggta cttatccctg tgactatagt ttggttaata gtaacctccc
    20821 tcatggtggt tgctcatgtc aagccatggt tttgacggtt agaagagaag ccaatgaaaa
    20881 aagtatatca ttatataatt cactatgttc gtacctactt actgttagag ctattagccg
    20941 gtttatggct aacagtaaag tattttttca ggaaaaaaat aacagttcag tttcctgaag
    21001 aacaaacgcc actttctcca agattcaggg gacttttggc attacgccgt tatcccaatg
    21061 gtgaggagcg ctgcattgca tgtaaattat gtgaagcagt ttgtcctgca ttggctatta
    21121 caattgaatc agagcaaaga gaagatggat cacgccgtac tactcggtat gatattgata
    21181 tgtttaaatg cattaattgt ggattatgtg aagaatcttg ccctgttgat tcgatcgttg
    21241 tcactccaat tcatcactac catatcagcg aacgtggtca aaatattatg acaaaagaga
    21301 agcttttagc agtaggcgat ttgatggaaa cacaactcgc agcagacaga gctgctgatg
    21361 aaaaataccg ttgatggggt gatttatgca tgaatgggta atccaagcaa ttttttacat
    21421 ctttgcatta ttgaccgtat tgtcggcact aatggtaata agccaaaaca atccagttcg
    21481 ttgtgttttg tttttagtgc tcactttttt taccagtgcg gttttatggg ttttagctga
    21541 ggctgaattt ttagcattaa tccttatctt ggtatatgtt ggcgctgtca tgactttatt
    21601 tctgtttgtc gtcatgatgt taaacataga catcgaaagc atgaaaagtc atttaattaa
    21661 atatctacca tttggattaa ttatagttgc cttgttgacc gggttattaa tggtagctat
    21721 ccctaaagat ttatttaaga acagtgtgca aattcaagaa aagcctttga gtgttagctc
    21781 acaattaatg gatttatcta ctcaagatga gctggttcca cctgaaaacg ctacatcgaa
    21841 tacagaaaag ttaggaatgg ttctttatac agattatatt cttgcctttg aaatagctgc
    21901 agtcattttg ctggtagcta ttgtgtctgc aattaccttg gtgcataggg gcgctatcag
    21961 gtcaaaacga caagatatca ctcagcaaat tatgactcga agggcagaac gagtgaagtt
    22021 gataagtatg aagtcagaga aatagttatt ggggaattct ttatgatacc tgtttatgat
    22081 tatttagtat taggagtgat tttattcggc ttgagcctgg ttggtattat gctcaatcgt
    22141 aaaaatatca tcttattact tgtttgtgtt gagttgatgc tcttggctgt gaataccaat
    22201 tttattgcat tttctcatta ctacaatgaa gtaggcggac aggtatttgt ctttttcatt
    22261 ttaactgtag ctgccgctga agcagcaata ggtttggcca tagtcatgtt gctttataga
    22321 aacagaggca atattgacgt tgataaaatg aatcatttaa aaggttaaaa tcgtgagtat
    22381 tcaacaagtc tgcttagtaa tcgttttgac tccccttgtt ggttctgcta tagcaggatt
    22441 ttttagaaac cagattggac gagtcggagc tcatactgta actattttag gcgtagctat
    22501 ttccttatta tgttcaattt ttcttgcgtg gggactgttt tctggttcga tcccaaacac
    22561 aaatagcctt gtttatcact gggctagcgg gggtgcttta attccctacg aatttaatat
    22621 tggcttttta atagacccgc taagcgttgt catgctggtt atagttaatt ttgtttcttt
    22681 actagtccat gtctacagta ttgggtacat ggcagatgat gatggttatc aacgattttt
    22741 tagttatatc tctttattta cattcatgat gctcatgtta gttagcgcaa acaactttct
    22801 gcaattattt tttggctggg aaggggttgg gcttgtttct tatttattaa tcggattttg
    22861 gtatcaaaag gaatctgcaa tagaaggcag ccttaaagca tttcttgtaa accgtgtagg
    22921 agattttgga tttgtattag gaataggttt aatttttgct tatgctggaa gccttgatta
    22981 tgaaaaagta tttaccagcg caaattattt agcaagccag aacatagagt tatttagcgg
    23041 ctactcctgg tccctgatta cagttatttg cctgctgctt tttgttggcg ccatgggcaa
    23101 atcagcacag gtgccacttc acgtttggtt acctgagtca atggagggtc caacccctat
    23161 ttcagcgcta atccatgcgg cgacaatggt gacagcgggt gttttcatga ttgctcgtat
    23221 ctcaccttta attgaattgt ctacagctgc gcttagcaca gtattagtaa tcggtgctac
    23281 aggagcattg tttacaggaa tactggcttt agttatgaac gacataaagc gcgtggtagc
    23341 atactccact ttatcacaac taggctatat gatggtggca atgggtgcct cagcctatag
    23401 tgccggtatg tttcatttat tgacgcatgc ttgctttaaa gcgctattgt tccttggtgc
    23461 tggttcagtc attattggca tgcatcatga acaagatatg cggaaaatgg gtggattatg
    23521 gaataaaatg ccgattacct atgtaaccta tgtcataggc agtttggcat tgtgtgcatt
    23581 tcctcctttt gctggttttt attcaaaaga taccattatt gaagcagcgc agctttccca
    23641 gatacccggt agcagttatg cttatttttg tgtaacagca ggtgctatgg ttacagcatt
    23701 atatactttc agatccttgt tcatgacatt tcatggcaag ccgcgcatgg atgaacatac
    23761 attaagtcat attcatgaat ctccatgggt agtatggtta ccactagtat tgctggctat
    23821 cccatcaata attttaggct atgtgcttta catgcctatt ctatttgata caccaaccct
    23881 cttaggctca tcaatttttg ttttacctga gcataatgtt ttagcagaac ttgctcatga
    23941 agtaagctcg ccatttgcaa gtgctgttca tgcgatttat tccttgccat tttggattac
    24001 agtactcgga gcaattattg cctgggtttg ttatattgcg gttccatcta ttcctggtta
    24061 tttagccagg tatttttcaa ttatttactc tattttactt aataaatacg ggtttgatcg
    24121 ctttaacgag ctgttctttg tgaaaggagc tagaggatta ggcattgctt tttacaagat
    24181 tggtgatcag aaactaatcg atggtgcagt tgtcaatggt agtggacgcc tggttcgctg
    24241 gttctctgga aaagggcgaa agatacaaag tgggtacatc tatcattatg cgacagtaat
    24301 ggtgtttgga ttactggcct ttttatgctg gttaatcctg gactgaagat aaggtgaagg
    24361 tatgcattat ttgctcaatt tattaatttg gttaccaata ttaggtggcg tttttgtttt
    24421 gctgactggg gatgataaaa atccaaatgt atcgcgttat ttaacattat tcaccatact
    24481 cctctgttta gtgctctgca tacctttagt taaaggattt gatctgcaat cttttggaat
    24541 gcagtttata gaggaaatcc cctggatgcc ggcactgggt attaattata ctcttggtat
    24601 tgatgggtta tctttactat taattgtttt atccattttt actaatttaa tagtgatttt
    24661 ggctacttgg gatagcatta gtaaaaaagt ttcccaatat ttatcagcgt ttttaattat
    24721 gcaagggctt ctggttggta tattttctgc tatggatgcc atagtttttt atattttctg
    24781 ggaagcaacc ttaattccaa tgtatctgat tataggtatt tggggttctg ataatagagt
    24841 atatgcggca ataaaatttt tcctgtatac ttttttagga tcagttctca tgctggccag
    24901 tttcctgtac atgggctaca ttgcaggctc atttaagatc gaaacgtttt atgccatcaa
    24961 attaggcatg actgcacaaa cattgatttt tattgccttt ttcttaggtt ttgcaataaa
    25021 aataccgatg tttccagtcc atacctggtt accagatgcg cataccgaag cccctgctgg
    25081 tggttcagtc gttttagcag caattttgct aaaactggga gcttatggat ttatccgttt
    25141 ttctttacca atagtccctg atgcttgcag caaatatgct cctctgatgg ttgccttatc
    25201 tttgatcgca gtagtttata taggactcat tgctattatt cagaaggata tgaaaaaatt
    25261 gattgcatat tcttcaattt cacatatggg ttttgtaact ctaggttgtt tcgctatatt
    25321 tgcaattgct cgccatggag gactaagcaa tgccggatta atccttgagg gtgcaattgt
    25381 cgttatgata tcgcatgcct ttgtctcaag tgcaatgttt gcaggtgtag gttttatata
    25441 tgatcgtatg catacaagac agctcgcgga ttttggcggg atagtaaata ccatgcctat
    25501 atttgcttct ttttttatgc tttttgctat ggcgaatgcc ggcttgccag gaacatcggg
    25561 atttgttggt gagtttatgg tgatattagg tagcataaag gctggttttt gggttgcatt
    25621 ttgggctgca actacattga ttattggagc agcatatacg ctatggatgt acaaaagagt
    25681 tatctttggt cctgtagcta ataaaaaggt tgctgcctta aaagatatat caggttttga
    25741 attaagtgct tatgtattgc ttgcaataat ggtcattgct ctaggagtgt atcccaaacc
    25801 catgttagag tttgtgcatc aaactgtaag tcatactttg gcattggctg ataaatctaa
    25861 actctaacaa agaactgcga taggcttaaa ttgcaatcaa gttccttagc aaggttaaat
    25921 atgatgacat tactagaaaa tatccatgta gcaataccag aaatgattat tttaataacc
    25981 gcgtgtttgg cgttgctttc tgatttgttc tttcgtcata aattaaaatc aattgcattt
    26041 tatatatctt gtgtaggaat tatagtttct gcagcagtaa gctttgtatt tataggaagc
    26101 tacaagttac ttatattcaa caaattattt ataagtgatg atataagtca tttaatgaaa
    26161 ctgtttattg atatcacggt tttattaagt tttgtctatt cccaaaacta tctggatgaa
    26221 cggcaaatgc ctacaggtga ttattatgtt ctggggctgt tttcaacact aggcatgatg
    26281 attcttgttt cagcacactc gctgttgtcc ctttatctag gtttggagtt aatgtcattg
    26341 ccgctctatg caatgaccgc tatccgccga actgatagtg atgcatccga agcggcaatg
    26401 aaatattttg ttatgggtgc tattgcttct ggtatgctgt tgtatggaat atccctggtg
    26461 tatggggcaa caggaaaact tgatttatta gatgttgcta atgcggtggc tgtcaattgg
    26521 cagcaacaaa atactctttt tgcgtttgca atggtattta tacttgccgg cgtaggattc
    26581 aaactggctg ctgtcccttt ccatatgtgg gctccagacg tatatcaagg ggcaccaagc
    26641 tctgtaacac tttttattag tacagcaccc aagattgctg cactggggat ggcactgcga
    26701 tttttaacta ttggcttagt cgacatcacc atgcaatggc agcaaattat tttagtcatg
    26761 gctctccttt ctaccggcat aggtaatttg ttagcagtga ttcaaaccag aatcaagcgc
    26821 ttgttagctt attctgcaat ttctcatata ggatatgcct tatttggagt tttagctgcc
    26881 agctctgctg gttatgccgc agcactgtat tatattttag tctatgctgt aatgtcagcg
    26941 gctggttttg gtttagttgt attgatgtca aatcatggaa tggaaattga tagtatcgat
    27001 gacttaaaag gcttgaataa aagaaacccg tggctggcgt ttatgatgct attagtcatg
    27061 ttctcaatgg ctggcgtgcc gccaacagtt ggcttcttta caaaattatt ggtattgaag
    27121 gcattagtag atgttcatat gacttggtta gctgttgtag gtttgctatt tacggttata
    27181 ggtgcctatt attatttgcg aattgttaaa attatgtatt ttgatcaacc tgaaaatgaa
    27241 aatatagtaa gaattggtac aggcaatacc atagtttatt cacttaattg cctctcctta
    27301 ctatatttag gtattttccc aggcgcttta attgcagcct gtattaatgc atttgcaaat
    27361 taattaataa actttaacaa aagtacttgc ttagggaccc aagtatcagt atacttgccc
    27421 tcagttgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgta
    27481 ggttcaaatc ctgcccccgc taccaattta taaaacccca tttttggggt tttttattat
    27541 gttgactagg atactgtcta aatctaagtc agttaccaga cttgggcgag tgcgcccttt
    27601 ttttgtttat acatactatg ataaatgatg acttgatagt gctattagag cctataatca
    27661 aaaatatggg ttatgaactt tggggctgtg aatatctttc ccaagggaaa cactcgttat
    27721 taagaattta tatagacaaa ccagatggaa taggaatcga tgactgtcag gaagtaagta
    27781 agcaagtcag tgctatgctt gatgttgaag accctattcc agggcattat agtttggaaa
    27841 tatcatctcc tgggataccc cggcccttgt ttagtatctg gcaatatcag cgttaccttg
    27901 gttatgaaat tcatgtaaaa acttttaagc cagtcaatgg gaagcgaaaa ttatcaggta
    27961 ttattgtttc agcatcagaa gatacaatag tattggatat taataatgaa catcaagaaa
    28021 tacttttatc aaatattgtg aaagcaaatt tgacagtcta gagaggcgaa caatgagcaa
    28081 agaattgtta ttagttgctg aggcattatc aaacgaaaga ggcgttagta aagacgtcat
    28141 tatacttgcc attcaggctg cactggaatc tgcaactcga aagattcttg gtttagatat
    28201 aggtgtgcgt atcaaaatag attctaaaac tggtgactat gaaaccttta gatactggga
    28261 tgtagtgaat gaagaagaat tagagtttcc ggaccgtcaa ctctccttgg agcaagcaaa
    28321 agagcgaaaa ccctcggctg ctgttggtga ccgaattgaa gaaattattc cttccataga
    28381 atttggaaga attgaagcgc aaacagcgcg acaagttatt atgcaaaaag tcagagaagc
    28441 ggaacgcgaa ttgatcatag accaattccg tagcaaactt gggcagctaa tctatggaac
    28501 tgttaaaaaa gtaactcgtg acaatataat tattgattta ggtggtaaag cagaggcttt
    28561 cttgccacgc tctgaaatgt tgcctcatga aatgtttcgt cccaatgaca gagttcgagc
    28621 atatctgtat gaaatcacgc cgcaggcgag aggaccacaa ttgttcgtaa gcagaactcg
    28681 aaatgaaatg ctcattgaat tattccgtat tgaagttccg gaaattggtg aaaatataat
    28741 agatatcaaa gctgcagcgc gcgatccggg aaatagagcc aaaattgctg ttaaaaccaa
    28801 tgatgggcgt attgacccca tcggtgcttg cgtgggtatg cgtggagcaa gggtacaagc
    28861 ggtttccagc gaattaggcg gtgagcgagt tgatatcatt ctatgggatg ataaccctgc
    28921 ccaattagtc ataaatgcga tggctccagc tgatattaca tccatcgttg tggatgaaga
    28981 tacccatact atggatttag ctgttgcgaa agaacaatta tcgcaggcaa ttggtcgaaa
    29041 cggacaaaat gttcgtttgg ctagccaatt aactggttgg acattgaatg tcatgacaac
    29101 ggaagagttt gaaagcaaga atcaagagga atcaagtaaa atagttaatc tgtttaccag
    29161 cgcgctagaa atagatgagg aaatagcgac attattggta gctcatggat tttcttctct
    29221 cgaagaagtg gcttatgttc cgaaggagga gctgctggct attgaagaat ttgatgaaga
    29281 gatagttgaa gagttaagaa acagagcaaa tgacaagtta ttggagatgg cactgtcatc
    29341 gggcaaagga ttatctggta ctccagatga ttcccttatc aatatggaag gcatgacaga
    29401 ggatttggca aataaattag caagtaaagg aattacaaca atggaagatc ttgcagagca
    29461 atctgtagat gaattaatgg aaatagaagg cattagtgaa gaaaaagcag gtgctttaat
    29521 tatgaaagca agagaaccat ggtttcaata aactatggtt atcgaaattg accaatacga
    29581 tgctagatgc attttaaata tattcagctt tataaaaagt tgctgagagg ggattaaata
    29641 tggcggatgt gacggttaaa caattagctc aagtcgttgg tatcccggtt gagcgcttat
    29701 tgaaccagtt acaggaagca gggttatctt ttactgatga tcaacagact gtgaatgaag
    29761 aacaaaagag gatcttgctt aatcatttaa aaggtagctc taatcgtgat attagtgctg
    29821 cgcctgaaag aataacttta agacgtaaaa gcatgtctca agtcactgtt gggcatgata
    29881 tgcatagtgg caaaacagtt aatattgagg ttagaaagaa aaaaactttc atcaagagaa
    29941 gcgcaattcc cgaacaagca gaggtagaag aacccgtagt tccacctgtt gttgaagagc
    30001 cagtccatga agaaatcaca gtggttagtg acgtgtctgc agaaactccc gaattgaaag
    30061 agacagagga gcatccggtt attgaacctg tagcagagct cgatgaaaca gtaaaagaag
    30121 aagaaaaaat caatttagag gaaaacacag cagagagtca ggatgagtta gcccatgcaa
    30181 atactgatgt aatagaaaat cttgtggatg ttgtcgagga gaccatccct gtttccaaga
    30241 aggaagaagt taaaccagag aaagtaagta aaaagaaaca tctggagcaa actgatactg
    30301 atatttctga atttaaaaag ggtaagaaaa agcctaaata tcatactttt gaacatgatg
    30361 aggaggagca agaacttcat cgtcgtggtg gtcgaagtaa atttaaaaag aaaaaaggaa
    30421 ctgaaaaatc ggataaatac agagaagctg aagaaacatt gacgcatggt tttgcgttac
    30481 ctactgctcc aatagtcaga gaagtattga tacccgagac aattacagtc gccgaactgg
    30541 caaaacggat gtcagtaaaa gccgctgaag tgattaaagt aatgatgtct ttaggagcga
    30601 tggcaacaat taatcaggtc attgatcaag aaacgtctgt aatcgttgta gaggaaatgg
    30661 gacataaacc ggtaattatc aaagaggatg ctgttgaaac cggtttaggc gaagcgattt
    30721 ccaaaggcac aaaaacagaa ggaagagccc ctgttgttac gattatggga catgttgacc
    30781 atggtaaaac ctccttactg gattacattc gccgaacaaa ggttgcagct ggtgaagcag
    30841 gaggtattac gcaacacata ggtgcttacc atgtcagtac gccaaagggt aacattactt
    30901 ttttagatac gcctggacat gccgcattta ccgcgatgag agcccgtggt gcccaagcaa
    30961 ctgatatagt cattctgatc gtggcagctg atgatggtgt taagccccag acaattgaag
    31021 cgatacagca tgctaaagcc gctaaggttc caatcattgt tgcaattaat aaaatggata
    31081 aacccgatgc tgatcctgag cgtgttatga atgaactatc cgttcaagaa gttattcctg
    31141 aggcatgggg tggagatacc atgtttgtta atatttcggc caaatcaggt atgggcatag
    31201 atgatttgct ggatgctatt ttattgcaat cagaagtatt agagttaaaa gcagtgactg
    31261 atggtgcagc aaaaggagtt gtcattgagt cacgtctgga taaagggcga ggtcccgttg
    31321 caacggtttt agttcaaagc ggaaccttgc ataaaggtga tattttactt gccggctttc
    31381 agtatgggcg tgtgcgtgct ttggttagcg ataatggaga tttggttgat agtgctggcc
    31441 catccattcc agtcgaagta cttgggttat cggctattcc tcatgcaggg gatgaagcgg
    31501 tcgttgtacc tgatgagaaa aaggccagag aagttgcttt gtttagacag ggcagattcc
    31561 gtgatgtgaa attggccaga cgtcaaaaaa ccacaataga aggtattatg gaaaatatga
    31621 cggctaccga atcgaaagtc ttgaacattg tcttgaaagc ggacgttcaa ggatccctgg
    31681 aagcaatttc tgatgcattg acaaaattat caacagatga agtcaaggtc gaggtgatat
    31741 ctagtggtgt aggcggaata acagagtctg atgtccattt ggctatcgct tccaatgcca
    31801 ttttaatcgg atttaatgta agagcggatg gtacagcgaa gcgtcttgct gaacaagaat
    31861 ctgtgtcgat tcattattat agtgttattt atgatatagt cgaccagatc aagggtgctt
    31921 tgacaggcat gcttgcgcca caatttaaag aagaaattat tggtattgct gaagttcgag
    31981 acgtattcaa gtcaccgaaa attggtgcta ttgctggatg catggttatt gaaggcgttg
    32041 tcaagcgtaa taatccaata cgtgtattgc gttcaaatgt agtgatttat gaaggtactt
    32101 tagagtcatt aaggcgattt aaagatgacg tattagaagt aagacaaggc tttgaatgcg
    32161 gtatcggcgt aaaaaactac aacgatgtga agcctgggga tttaatcgag gtttttgaga
    32221 cagttgaaat taagcgtgac ttatagaaaa tgaataataa ttttaagcga actgacagaa
    32281 ttgctgagat gattcagcgc aaattagctt tgatcattcc tcaggaaata aaagatcctc
    32341 gcttgaaggg ttttgttacc atttctgcag ttaaagtggc tgcggattta ggtcacgcca
    32401 aaatttattt tactgttctt aatgaagata aatcggtggt gaccaatata ttaaatggtg
    32461 cagccagtta tttgaggagc gctctggcaa gaagtattac tctacgcact gtgccgcaac
    32521 ttcattttat ttatgatgaa tcgattgagt atggacagcg gttaagccga ttaattgatg
    32581 aagtaaatcc ccctgattca agtagtgatg acaacaattg aatctcagtg ttccatagat
    32641 ggaatcctgc tattaaataa accacaagga atgacctcta acgctgctct tcagaaagcc
    32701 aaacatttgt ttggtgctaa aaaagctggt cacacaggca gcttggatcc tttagcaacc
    32761 ggtatgttac ctctatgctt tggagaagcg actaaaatat gccagtatct ccttaatgcg
    32821 gataaaagct atgaaacaat tgggaggcta ggtagtaaga caaatactgc tgattgtact
    32881 ggcgaagtga tattttgcat tgaaaattat actgttagcc atgaggaaat gattgcaacc
    32941 ctagaaaaat ataagggtaa aatcaagcaa attccatcca tgttttctgc tttaaagcac
    33001 aaggggaccc ctttgtatcg acttgccaga gaaggtatag agatagaacg taaggctagg
    33061 gatatagtaa tcagccaatt gaagttggaa caatttgatg gtgaatgttt tacattaacg
    33121 gtatcatgca gcaaaggaac ctatatacgt aatttagtgg aagatattgg tgatacgttg
    33181 aaggcaggag ctcatatgac aaaattgcat aggctctaca ctgcagggtt tgaaaataat
    33241 cgaatgtaca ctctcgatga attacaggat atgcctttat cacaaaggct tgcttgctta
    33301 atccctattg atcaggccat tcagcattta acaccagtaa ttctttcaga ttctgaggta
    33361 acagcaattc gccagggcaa ggtgatttca aataaaactg gtgctgttga aggagaagat
    33421 ttgcgccttt atggcgagca atcccaattt attggcattg gccaagcttt aattcatggt
    33481 gatattaaag caaaaaggtt agtttctttt gcgttgtaat gacaattttt gtaagaattg
    33541 ctgcctactt tgtaataagt atgaaatttt ttacacaaat ttatatccac tacttgtttc
    33601 atttgacatg ccttggtaga atgttcgcct ttaaaagaat acttagctga gttccaggag
    33661 taaataatgt cgctaaatag cgcggaaaaa gcagaaatta ttaatgaata caaacgcggt
    33721 gataaagata ctggttcacc tgaagtgcaa gtttctttaa ttaccagtcg tattaaatat
    33781 ttaactgatc attttaaaga aaataaaaaa gatttccatt cgagacgtgg cttgcaagaa
    33841 ttggttaaca agcgccgcaa gttactaaaa tatttgaagc gtaacgatca agatcgctat
    33901 caaacattaa tccaaaatct gggattgaga gattcctatt gattgatagg attattgtct
    33961 ctatggggac gcatcaaata ataaatcagt tttagaataa aaggcgcagc gttctgcgcc
    34021 ttttttttct tatatttact taatggggaa cattacgtgg caaaaattac aaaagaaata
    34081 gtattcggta accataactt aatcttggaa acaggtgaag tggcacgaca ggctgatggt
    34141 gccgtaatgg caagtatgaa tggtacacag gtgctagtga ctgtagtttg gaaaaaggat
    34201 ggtggtgaaa gtaatgattt tttcccatta acagtgaatt atcaagaaaa gttctatgcc
    34261 ataggtaaaa tccctggtgg ctttaacaaa cgtgaaggac ggccatctga taatgaaaca
    34321 ttaatttcgc ggttaataga tagaccgatc cggccattat tcccggataa tttttttaac
    34381 gaagtacaga taattgctac tgtcctgtct ttaaatcccg aggtatctcc tgatattatc
    34441 gctatgattg gagcctccgc agcgctttca atttctggcg tgccatttaa tggccctata
    34501 ggcgccgctc gagtaggcta taaagatggc gtttatctgc tcaatccaag tagaaaagaa
    34561 caagaagaat cgaagcttga tttggttatt gcgggaacaa aagatgccat tttgatggtg
    34621 gaatcagaag cgcaggaatt aagtgaagac atcatgcgtg gtgctatgtt atatggccat
    34681 gaaatgatga aaaatgtcat aaaatcaata gaagaacttg ccagagaagt gggtaagagc
    34741 aaacctgaat ggaaagcgcc agaaattgat acagtactaa aagctagaat caatgacgta
    34801 gcgaggaatg aagttgaagc cgcttatctt attaaagaca aacaacaacg ctaccagcga
    34861 ttagatgagt taagagagca aactatttct gctttactgg ctgaaaatga tgagttaaat
    34921 gcagatgtta ttgccaatat gtttggtgag ctagaacgtt ctatagtacg taatcgtatt
    34981 cttgacggcg agcctcgcat cgatggtcgt gatcacagaa cagttaggcc tatttctatt
    35041 cgtaccaaat tcttggaaag aactcatggt tcctgtttgt ttaccagagg agaaactcaa
    35101 gctattgttg ttgccacttt gggtaatgaa cgcgatgcac agatattaga cggaattagt
    35161 ggcgaaagca gagatcgatt tatgcttcat tataatttcc ctccttattc tgtcggcgaa
    35221 acagggcaag tgggtagtcc taaacggcgc gaaattgggc atggtcgttt agccaagcgt
    35281 gctttaatgg ctgtactgcc tgatgctaat gagtttcctt atgtgctacg tattgtatct
    35341 gagattactg aatccaatgg ctccagttct atggctactg tttgtggaac aagtctggca
    35401 ttgatggatg ctggggtacc tttaaaagca ccagtcgctg gtgtggccat gggcttaatc
    35461 aaggaaggtg atcgttatgc tgtattaaca gacatattgg gtgatgagga tcatttaggt
    35521 gacatggact ttaaagtggc cggtacggaa aaagggatta ctgctttgca gatggatatc
    35581 aaaatttccg ggattaccaa tgaaattatg gaacaggctc tggagcaagc attggaaggt
    35641 cgtacccata ttcttggtgt catgaataat gctcttgccg agcatagaac cgagttatct
    35701 cagcacgctc caagaatcac aactatgaaa gtcgctgaag ataaaatccg cacaattatt
    35761 ggcaaaggcg gtgcaacaat caaaggactt attgaaagta ccggcgtatc tatagatatt
    35821 gatgactcag gggttatcca attattttct ccggataaga tggctctgga agaggcgcaa
    35881 aaacaaatta aagctttaat tgctgaaatt gaagtgggtc agacttatca aggaaaagta
    35941 agcaagatag ttgattttgg agcttttatt aatttactgc ctggtaaaga tggcttacta
    36001 catatttcac aaatatgtgc cgatagaaca caaaaggtag aagaggtatt gcaggaaggt
    36061 caggaaattg aggtctttgt tgctgggata gataagcaag ggcgagtgaa actggagtgg
    36121 aaggataagc cgcaagctga agccaaagag gtcgaagacg ccccggtatc tgctacgttt
    36181 cttacaatgg aagagcaatc tgaagaaatt aattcaggca acaaaatctc tgaagaagag
    36241 gaataataag atagtagcat ttcaagttct attcattata aactcgtgga aggcaagagc
    36301 ttttcgcgag ttataactga gtgtttgcaa ttggtgtata tgaccgcata aattataagc
    36361 tactaaagta gagtttaagc atgaagctat tctctttttt tcttgtattg ataagctttg
    36421 ctagcttagg tcagggttta ccattacatc tcctaaagtg cccgcccgga ttttcagtaa
    36481 gtatttatgc ggcccccgtt tctgatgctc gagaaatggc actgggcagt aaagacatag
    36541 tttttgtagg ttcaagaagt gccgggaaag tgtatgctgt tattccagat aaaaaaaaat
    36601 cccatggtac tcgagtttta accatagctt ctggattgaa tatgccaaat ggtgttgcct
    36661 ttcataaagg cgcactttat gtcgccgaga ttgggcgcat tttacgcttt gataatattg
    36721 aatcaaatct gaataatcct cctgaaccag ttgtggttac tacaaaatta ccgaaggaaa
    36781 aagctcatgg ctggcgtttt attagttttg gaccagatga taagctttat ataggaattg
    36841 gcgccccttg caatgtttgc ttaatggaag atacgcgcta tgccactatt atgagaatga
    36901 atgccgatgg gcaagatctc gagatctatg ctaaaggaat acgtaataca gtggggtttg
    36961 attgggatcc cgttcatcat aatctttggt ttacagacaa tgggcgtgac tggctaggtg
    37021 atgacttacc tccagatgag ctcaacgtag ctccgagaaa gggaatggat tttggttttc
    37081 cttattgcta tggtaaaagc acagcagatc ctgtttatgg caaaacacat tcttgttccg
    37141 agtttactcc tgcggttttt gaattcccag cacatgttgc tcctcttggt atgtcatttt
    37201 atacaggtaa attgttccct cccaattatt ggggacaaat ttttattagt gagcacggct
    37261 cctggaatcg tagtcataag atagggtatc aagttactac agtaaaaatt aagaataatc
    37321 acgtaactga ggttaaacca tttatcagcg ggtggttaca gggggaaaaa gcatggggaa
    37381 gacctgtcga tacgctcgtt atgccagatg gatcactatt aatctctgac gattaagcta
    37441 atgtaatata tcgggttagc tatcagcagt aaatggagca attgttttat tataggcggg
    37501 gataaagccc ctacctattt cttatcagta ttaacggatt aaatttaaga attccaaacg
    37561 gcaactcgaa ttgttacgca tttcaccgag catcactgaa gttgtcatga cagaattttg
    37621 tttttcaact ccacgcatca tcatgcaaag atgttttgct tcaatcacta cggcgacacc
    37681 gcgagctccg gtaatggatt caatacaatg agcaatttcg ccagtcagcc gttcttgaat
    37741 ttgtagtctc ctcgcataaa aatcaacaat acgagcaatt ttagataagc ctattacttt
    37801 accatcaggt agatatccaa catgacattt gcccaaaaaa ggcaataagt gatgttcgca
    37861 taatgagtac agttctatgt ccttgacgat aaccatttca ctcatgtctg aatcaaacag
    37921 agcaccgtta atgatttcat caaggttctc attgtaacct ttagttaaat aacgtaaggc
    37981 attagctgct cgtccaggag tatcctttaa tccttcccga tttatatctt cgcctaattc
    38041 tttaagcaaa tttgagtata atttttccat agcaagaatt cctaacctgg cggccaagtc
    38101 atttgacgcc cgccaagtaa atgtaaatga atgtggtaaa cttcctgccc gccaccagaa
    38161 ttaacattaa aaaccaatcg ataacccgtt tcacttagtc cttcggcctg cgctaatttt
    38221 tttgctctta ttagaatatt agcaagaagt tcgctatcat catcgttcac atcattgata
    38281 gttgcaatat gctttttggg tataatcaat aaatgtgttg gtgcttgagg tctaatatcg
    38341 cgaaatgcca aaatattttt gtcttcaaaa actactgttg caggaatttc accctgtgca
    38401 attttgcaaa acaaacaatt cataggtcat ccgatgtttt agattcaaaa tgctattata
    38461 catttcattt attatattgc gaacgcttgt ctttgccttt tgatgcgatt tttcgcataa
    38521 tattactttt atttattatt aagggtgcgt aaatgagttt aatggaaatt cagagcggtc
    38581 gtgatgttcc aaatgaagtg aatgtgatca tagaaatccc tatgcacgga gaacctgtaa
    38641 agtatgaagt tgataaaaaa actggcgctt tatttgtaga tcgatttatg acgacagcaa
    38701 tgttttatcc aacgaattac ggttatatcc cgaatactct ttcggaagat ggggatcctg
    38761 tggatgtgtt ggtaataact cctgtccctt tgatcagtgg ggctgttatt tcatgtcgag
    38821 ctgttggcat gttaaagatg acagatgaat ccggagttga tgcgaagatt ttggctgttc
    38881 caacaaccaa actaagtaaa atgtatcaat ccatgcaaac ttatcaagat attcctcagc
    38941 atttattgtt atctatcgaa cattttttta agcactataa agacctggaa gaaggtaaat
    39001 gggttaaggt cgagggctgg gtaggcccag atgcagctcg tgaggaaata acatccagta
    39061 ttaaccgtta taatcacaca aaaaagtaaa acagtgactt gaactgtgcc aggtgttggg
    39121 cattgctcag caccaacctt atatctttag ctataatagc ggcatcattt cagcccctac
    39181 tacaaaacag caagttctat tgtataattt gtcattctta aaagtataag gatattcagt
    39241 gcaacttcct ctttctactt tattggtttt attgtttttc ctaatttgcc tgtctgcttt
    39301 tttttcaggt tccgaaattg gcatgatgtc tatcaataga tataaattgc gacacatggt
    39361 aaaaagcaat gataagcaag ccattcgggt taattccatg ctggctcggc ctgataagct
    39421 actaagtgtg gttttaattg ggaatacttt ggcaaatata gtggcatcaa ctctcgctac
    39481 tttaatcgga cagcggtttt atggggatgc aggggtggct atagcaactg ccttgttgac
    39541 catactgatc ctggtttttg cagaaatgac tccgaaaacc ctcgctgcta tttaccctca
    39601 acaagtagct tttgcatcct cattaccgtt gagcatttta caatggattt tttctccttt
    39661 ggttcattta atcagtttga tttccaatgg tatcttaaga ttatttaata tatcagtaaa
    39721 acgcatgcaa aaggagatgc taactggtga agagttgcga tcagtggttc atgaggcggg
    39781 aggattaatg ccggtagagc ataaaagtat gttgattagt ttattggatc tggaacaagc
    39841 aactgttgaa gacatcatgg ttcccaaagc cgatattgtt gggattgatt tggatttacc
    39901 ctggatagaa cttttagatc aattggaaac agcacaacac acaagattac caatttatca
    39961 tggttctatt gatcatttgg tagggatgat ccacgtaaga aatattttaa atttagcatt
    40021 agaaaataag ctggatatgg acaatttgtt aaatagtgct gaagttccct attttattcc
    40081 cgaggctacc ccattaaata tacaaatcct taatttcagg aaaatgaaaa gaagaagttg
    40141 ttttgttgtg gatgaatatg gcgatattct tgggcttgta actatggaag atattcttga
    40201 agaaatagta ggtgaattca caacagatat cgctgatctg agtcgtgata ttacacctca
    40261 gcaagatggg tcggtcatag tggatgccag cataactatt cgccatttaa accgcatgat
    40321 gggctggagc ctgcctttaa tcggtccaag aaccttaagt ggcctgatta ttgagcattt
    40381 aggatatatt ccaccccctg actcttgtct ggttattgat cattatcata ttgaaatatt
    40441 aaggatcagc gacaatatga taagaggtgt aaagatgatt aaggctaata aaagaagaaa
    40501 atagctctta tagacactga ttctcaattc gcttccagct tgtccctatt ttagcttact
    40561 caaatattta tgcattgata aagcgatata attgattcat tttatcgcaa tatatttctt
    40621 caatgcagat attaagatct cactttcgag gggtgagata ataataaggg taaaaaaata
    40681 acaatatata tatttttgac tcatttatgt taaaataacg cctttataat atgaaataga
    40741 acagatgaag attttatatg tacctttccc ccggaatcag gctggcgatt taaaatcaat
    40801 ggttgaactt tggaaaaaaa acgatgagca attttcccct gatcgcgttg aaataattta
    40861 tttcaatgat gacattaatt atgatcaatt ggatgaaaaa atcacgatat atatttgtgc
    40921 ccatggttca gatgatgaga atttaacaaa actatttaat cattccaatc cattagttgc
    40981 agagagcatt gatataaaag aagtggcaga acgctttgaa agagatattt tacctatatc
    41041 gtattggata tcagcggttc atctttattg ctgtggaagc aagaataaaa atcagatgat
    41101 ggctgaatta ttagggcatt cattgttaag accagaaaag cccatttatc attacagtgg
    41161 gtcagtttct atcgttgacg agtttgggaa acaatggagt tttgcaaacc atgttaaaat
    41221 acctgtgcat ttgacagcta aaagaatgtt tgttctgaat ttttttgatg aagagcaacc
    41281 ccatagagcc tctgttaaaa aagaatttca aaacaagact tataaagagt tattagccaa
    41341 gaaggaagat tctttttttg cgaaagtgaa agaaaataga gcttcagtgc ttttatccaa
    41401 acgattaggc aaaaagagcc aggatggtga agataactct ttgttgagaa agggtaatta
    41461 aagtttttac ctttgataca ttatagtttg cggcaattcg ttataccaaa agacattctg
    41521 tgaagtgcga taaccattga actcagaata agagtaagca ttttgatatg atgatgtcgt
    41581 tgccaagagt tataataaga ggctaaaaat gacttaaaag gaggccagtt agcgatggga
    41641 ctggtttatg gagttacaag aaaatggcat ttaatatttg acaaattatt tttttaagct
    41701 aaccttgttt ttgtgcattg caacatatgg agagtgtaat gaaagagcaa ttttttcaga
    41761 caaaagattt aaatactttg cttcgatctg ttgaaaaacc aattcaggat ttgttagatc
    41821 tcaattttaa aacattaaaa actgtgtctt atgttactcc tattgaatta ttcaatgttt
    41881 taaaaccaga agaagtccta gaaaaaaata tgaatgtatt cattcaaaat agtcaaaaag
    41941 caatgagtta catgttgaac gtatttcaaa taatggaaca tcattggatt aatatctatg
    42001 atcagatggc cgaaaatcaa aatgaaaaga aggctaatcc agcaataaat aaggcaaatc
    42061 gcaaagtaga taccagtact ccaaaatcat cagccagtaa aaaagccgtt acagcaaaag
    42121 gtaaaacaaa gcctgctgca gtacagtcaa aatcaaaaac aagcaaagcg aaactgaaaa
    42181 tcagcagtcc aacaaaagtt aaaacaaata ctcaagagcg gcctgcagct gtcaaacaac
    42241 aatcgcaaat gcccttatct tctttgcaga aagatatgga aaaacagggt attaacacgc
    42301 ctgttgaatt gaaaaactca gttgcgatat taaaaaatga aacaaaggat atagtgtctt
    42361 ctaataacaa acttaaataa actggtgttt tatcctgttt tcgttttgag cgcttttttg
    42421 agaagtagca aacgatactt gataaatccg ggatacccaa aagctagaca tggtagggtg
    42481 caatgccagc aagtaaagga taatgcgggc attgtgccaa taaagcaaat atcagttcat
    42541 agttattaaa atgcactcga taaataagat gaacgaattt gtctaaataa gccagataat
    42601 aatcaattat aatgtggcgt cactttcttt aagggttaca actcgagctg tgaacatgta
    42661 gccgccattg cgaatggttt tgattaaagc tggctttttt ttatctattt cgattttttg
    42721 acgcaaacga ctaatttgga catcaatcct tcggtcaaaa ggaaccaggt ctgtattttt
    42781 ggtaatttgt aataaaaact ctctatccag gactcgttgc ggttgttgga caaaagtaag
    42841 aagcaaatca tactcaccgg cgcttaattg caattcttca ttattctccc cgaacacttg
    42901 tctggaagct ggataaagtc gccagttagc aaacattagt acttctttac catggtctac
    42961 tttttgttgc gccctgatta ctcgacgaga aatagcacca attcgcgcat gcagctcacg
    43021 gggataaatg ggtttggtca taaaatcatc agcgccggct tcaagcatgg caacgcttga
    43081 ttcttcatca ggtttattat taataacaat taatggcact ggatacatat tataaaattg
    43141 ttcaattgct tctggttcag ttcttaaaat tgaccaatta attaaaatgg ctgtaggtaa
    43201 tcctttttca acatgaatag gaaacaattt cttttgttga ataatatcaa gattaaactt
    43261 ttcaaagtaa tctttcagtt cttcactgat aggctcatta tcaataaata gcaaatagtg
    43321 gcgttgtgac atcaatttgc ctcaaaggtc aaaaaatata ttgctttaat tttggcaagt
    43381 ttatcagttg cttgcaatta tttttaaata tttatgcttg ccagagcttt ttagagcgca
    43441 aaacctggtt tattacaaaa tttgcatttt gttcgttata aaaattagga aaatatttct
    43501 tttcaatgct attgttaaag ttcaagtgtc aggataatgc ttgaggagaa agggatgact
    43561 caaaagacac ttactttacc aaattggttc gttacggata atgacagagc catggcgcga
    43621 atcaaatatg agttgaaaca gttggagact cagcttggca aatgcgttaa gcagccggat
    43681 gatactgcct tttcaaagaa caagtactca ctttacatgg ctttagcgga taaatattcg
    43741 gagtatttga agttaataga tattgattct aaatttaaac aattcgtagg ggcggagata
    43801 cctaaggata ttagttattg ggaagcttgc actcggatta tgcatctgaa acagaaaagg
    43861 tctgcagcag tgaatttgct ggaggaagct aaaattatca ttgagatatc attattgtat
    43921 caatgtcttg ctaaagcaat tgaaccaaat gaggtttcag acgtaagcaa tcatttaatc
    43981 ctgaatcagt ttgataaact aaagcagtta tttaagaaag agaatcttga attatggtta
    44041 tcaactctgg ttgatctttg gaatcacaat tctactttat taagggagcc agccaagtta
    44101 tttcgcaatt gggatcttga agaacaaacg atagccttag gttttttttc tgaatctgaa
    44161 gttgttaatt tagtaaatgc tatatttttt tataaattgt atcctgataa gttatttaat
    44221 gagttaattc atcctgaaaa attggtttca gtgcgtatgc gccttggttt tttgcatgaa
    44281 tttatagaac ttctacaaaa acaattaaat agcaatgcgc ttcaatatgg gctaaatcca
    44341 ggtattgatt tcctatttca tggtgaagaa ctaccccaag gcattatgat cgaagtgaat
    44401 gaagtttata gaggtataat ccaattagct gttaaaaacc ttagagtgca atttacacct
    44461 gaaaataatg agaaagttac cctggaacgt ttacatgatt tgggaagggc atacaaattt
    44521 tggtttaatc ctaataggct aattgacgct gtgatggttc tgcagcaacg tttggtaacg
    44581 gaaagtatat ctcaagagga ggatttggaa aaatttcatc aagaaatgct agttttatat
    44641 cgtcagctca ccacaacaga atgcctcgat ttatacggtt attttgccaa caatgacagt
    44701 cgatatttac tttatacttt atattcaata aacaatggtt actctttaga ttggttgcca
    44761 tcattaaatt catctgaaag aaatgctata gccagagtgt atcaagcgtt gaagtgcgtt
    44821 atggaggcgc taagagtcga attaaaaaac agatttatca caaccgaacc ttatctatat
    44881 gatttgggga aaaaggaaat tcatacagga agacgtaatc gagaggctgt atttagagtc
    44941 attgcgattt atggttgtga aagaatagtg atgagtgact cattagaaag attgtttaat
    45001 ctcattgaag aaccaaatca gcaatttgag tagtaaatat gaatttttca ggtgccaatt
    45061 gactaagagt aatcaagtta tgcaagaggt ctgctgtttt gtatgggcgc ttttgcagcg
    45121 aactttataa agcgctttta ttgaatggga taagccagtt ttttaccata gaactcttga
    45181 agttgtctct ttagtttttc tgctggacta gatttggcaa aatcactctg caggttatca
    45241 atccattctc cagttgaaag attgagaata acaggtatgt aggtgttgtc atctggatat
    45301 agggctaaaa tccagtttcc actaatgacc ccgtatagct tttgattatt cacctggatt
    45361 aattcatcag gcttcaattc aagttgccca gtatctggat taaccttata gatttttttt
    45421 cctaattcaa tggctttttc tttagtaaaa tcttgatttg gatacattcc actttcaata
    45481 aagaatgtgc ggtgtggtag tggagtattt ggatttaata cagtaggtag taatgaaatg
    45541 ccatccattt tctgtttttt tgtaatattc aggaagtcca gaatggttgg ggccaaatca
    45601 aataatgcta ctcttgtttt gattttcttt gtttttgtaa gtggcttccc atctttataa
    45661 atagtaaatg ccaggaggct gtgatattgc ataggactta atatatcaga accatgacca
    45721 gcacttttat ccagttcggt tgcagttttg gccttaaaat actctgcgag tttgctagtt
    45781 agtgagcttt ggtatttttg gtaatttgtc aatcgtgagt tgggataata aagtacttcg
    45841 ccatggtcgc ttagtataat gaccaggcta tttttaaaat agccatgttt ttttaaataa
    45901 cagaaaatga ttttaaattg attgtctact gcttttagcg ctcttaggta aagcgcatca
    45961 cgcttactga ggctgaattc attctccact tgatcaggca atgatgctgc ccaggcataa
    46021 ggccaatggg gaagagcgaa atgcaccgct aggaaaacag gagaattttg ttgtaaagac
    46081 aattctcttt ttaattcatt tgtaaaagtt tccggatagt aggatatgaa gctggctcta
    46141 ttactaaaat tataggggaa taaccaggcg ctaatgcgaa aattaatcag taaattccca
    46201 agaggaaaat cattataaga gcctaacatg acatcattga cacctgtttt tgggccgatg
    46261 atttttttaa atccaaaatt ctcatctatg ctattgaatc gtctatcatc agtcgcatag
    46321 attgtgttat gccccaattt attcaaatcc cacacaatgc taagttgact tttaacccgg
    46381 cttcgctcaa ctaaattttc ttcggcaaga tgatgtttag ggtataaccc agttaaaata
    46441 gagctccaag ctggataggt tctggctaaa ggactaatcg tgtttgtaaa ttgaaaactg
    46501 tccagtagca cttgatggag aaaaggcatg tttctagatg tgacactttc tggacttaag
    46561 ctgtctatgc ccagaataat gacgttgggt tcggaatcaa catttggatt taaggaagat
    46621 ttccaataat tatttaatag gaaaatacaa atgccaatgg gtagcgctat tgataatagg
    46681 cgagccgtcg tgcgataaaa caagcaattt aataagagta accccaggca agccaaagaa
    46741 aaataaagta atattaaaaa aataaactca ggaattggcg gtgaaaatag tttgctaaaa
    46801 acactcagag ggaaataata ggcatttgca cttaatatag cgcatgcaaa taaagaccaa
    46861 ataatggttt gccattgctc agtagaaaaa taatgccaag atcgatttaa aatgcctata
    46921 agcaacagag cttgcatcat ggaaatgatg atatacaggc tgaattgaat tacgagagtg
    46981 ccgataattt caaaatagat tggcaaagga agcttaatcg aactgattag gctctcagca
    47041 tgcgaataga gcagaagcaa ttgcaagcca aaaaagcaaa gattaaataa aaggaattgg
    47101 ctaaaatatg caaaaatcaa atgctttttt ttcatgattg attgtctgca ggacaattaa
    47161 tatgccagtt ataaatataa ctatcaatag tattaatatg ctttaattgg ttacgcagag
    47221 gttctttcgc gaattgtagc aaatgtttga tgacatattt tttggtacca ccaaaatctt
    47281 cttcatacca gtcatataat ttagaaacaa ttaatttacc ttcaatcaca tgaactcccc
    47341 tgagagagtt aatgtatttg aatgccgcgt cgttaagttg ttgctccagt aatggccctt
    47401 ggtatgcttg tttgctcaga ttaggcgctc ctatggtggc attatttaaa gcataatgag
    47461 ttcttgggtc attccaaatt gggcgaataa ttctattatt gatatcatcc agggataagt
    47521 tagtattttt gattgtgata atattggctc cccaaggtcc aacgctaaat aatccgggag
    47581 aaatgttaat ttcctgaatg tttgcaatgg ggtaatagtt ggctactgtt aataccgtta
    47641 gggcattata caaattgatc caataagcca attgttcttg acggttataa ttatcaatat
    47701 cgatttgcga taaatttttt atatattctt ttagtgaagc cagctctttc tcattgatat
    47761 tggtatagtc taccagattg atgtcttctt cattagttat tacatgatga tcaagaaaat
    47821 gttgccatgc ctgatgagaa attgattgtt tggaaagagg agcattgacc aaccatttgg
    47881 gccataaact tctgtggaag gaagcagagg ctgtacttgt tatgaaacat aaggataaca
    47941 ggagtaagac cagtattttt tttctgcata aaataaatat ggatttaaac attaatcaca
    48001 gctctgttct taagggcact gcttatagta ttcccatcta aaaattctaa ttcaccacct
    48061 gatggtattc catgtgctaa ctgactgata ttgacggttt catcctttag caactgatga
    48121 ataaaatgaa ttgtggtctg gctttcaaca ctggggctta atgcaagaat aacttcctga
    48181 attttttccc taataattaa ctccttaagt ttcggtagcc ctatgtcatc aggtcccaag
    48241 ccatcaagag gagaaatttt tcccataagg acaaaatatt taccttgaaa ggaattgctt
    48301 tgttctatgg cagaaacatc cgctggactc tctaccacgc aaagtaatgt tgaatcccga
    48361 ttggggtttt ggcaaagagt acacagagtt tgttcagtgt aattattaca ctgctggcaa
    48421 tgactaatgt gcttcattgc ctgttccaaa caggaggcaa ggtgtaaacc tcgctgtctt
    48481 tggtgctgca ataaatggaa caccatcctt tgtgctgatt ttggtcccac accgggaaga
    48541 cagcgaagcg cctctaccaa gcggcttaga gcatccatag ccagggttac tctccttctt
    48601 taccacccat taaatcagtt ggaatattaa ggccagcagt cagttggctg attttttctt
    48661 tggatgcttt ttcgattttg cgcactgcgt cattgactgc agcagcaatt aaatcttcca
    48721 gcatttcaat gtcttcatcc attaaggtgg gtttgatttt aacttcggtt acatcatgac
    48781 gtccattcat cttaatagta accatgccac cacctgattc tccacttact accaactggc
    48841 ttaattgttg ctgcgcttct tgcatacgtt gttgcatttt ttgcgcttct ttcattaaat
    48901 tgcctaaatt ttgattaata tccattacag cctcgtctat ttatggttag tttaagtatt
    48961 ataagtcatc ttttaacagg acaatagaat ttttgaccaa ttctgctgaa aattcttgtt
    49021 ttaactgttg aaataccggg tcagcgtgta atgctgcctc tgcttgttct tgcttttctt
    49081 gggtcgcttt ctctttctgt tgagctggag aggcatgcac ggcatcttga ctgtttaaaa
    49141 taatttttac gggactttta taatacccgg ccaatgcggt ttcaattcgg ttaatcgctg
    49201 tgggagtgaa taaggactgg tgccctttct gtactcccaa tgtaatttct cgctcttttt
    49261 taccaagaaa ttctgcattt tctatggcat taagtgctaa acccgataat tttaactgtg
    49321 gtattatgtc gttccagtca cagttcatgg actcaccaga gttggcagat tttttatcgt
    49381 ctgttgataa tgattgggtc tgatgatagg caacggtgtt gtccgtcgct ttagtaacgg
    49441 gtattggttc aattccaata gggttttggg ctggtgtatt atcatcaaca attagtgagc
    49501 atgttttatt attttgctcg aatgctaatg gaggtgttgt tacatgagaa gctggtttaa
    49561 aggtatacat gcgcagcatg gtcatattaa agccaatggc cagcgtaggg gcgagatgca
    49621 tctcctcacg tcccttaata cctatttgat agaataattg tgtgtcctcg gccgaaaact
    49681 gttttgctaa ttctttaatt tcagcttttg gactaattaa tggattgttg tcgcccaggc
    49741 attgatatac ggtaatttga tgcagatagt taagcatttc atcaagaacg tattgaaaat
    49801 tccctccttc ggcaataatt tgctgactta atacaagcat gattggcgct tcttgcttgg
    49861 ccagggcttg caagagcagc aacgcgtaat cttgttgggt atgtcccaag agcattttta
    49921 catcacttgc cagcaatttc gtttcacaac tggtaatggc ctgatccaat aaacttaatg
    49981 catcgcgcat gcttccgcgg gcagcctgag ccaatatgtc tagcgcttgt atttcaaaat
    50041 ccaagcgctc ctcttggaga attaattgca attgttgatt tatcaactca gcaggcaagt
    50101 gttttagatt aaattgcaaa caacgcgata agacagtaac aggtaatttt tgtgggtcag
    50161 tcgttgccag aataaatttc acatgcgaag gcggttcttc taatgttttt agtaaagcat
    50221 taaagctatg ctgggatagc atatgcactt catcaattag ataaatttta aaacggccat
    50281 ttgtcggtgc atattgtatg ttgtcaagta aatctcgcgt gtcttcaact ctggttttgg
    50341 aggctccgtc tatttcaatg agatcaatat aacggccttg ctcaatggca acacaggtat
    50401 cgcaatgaag gcatggttct gaggtgatgc ctttttcaca attgagggct ttagctaaaa
    50461 tacgggcgac gctggttttc ccgaccccgc gagtaccagt aaatagataa gcatgatgca
    50521 aacgttgttg attcagcgcg ttgatcagtg ctttattaat atgctcttgg ccaaccagtt
    50581 gggaaaatgt gcgtggtcgc catttacggg caagtgccag atagctcata cgtgtttcac
    50641 aagttgggcg gcgactctaa gagccacacc ccggcgcccg catcaattgc taccgctgct
    50701 cccttccggg cctgacgggg ttcacaatct atcgtcgcga ggggaccaat agagtcacca
    50761 tatttgaata gcgaagagta acaaaaaaaa tatttctgcg ctagagctaa tgactatttt
    50821 attatttgag tgtgactgct ttcctaaagg atagaaatta aaaaagtaat taataaccag
    50881 ggctaataaa gagcatatcc aatccacatg aataaatatt tcttttgaag cta
//
LOCUS       DATNSN010000006        50006 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.006, whole genome shotgun sequence.
ACCESSION   DATNSN010000006 DATNSN010000000
VERSION     DATNSN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 50006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50006
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.006"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            66..806
                     /locus_tag="VAH76_000273"
     CDS             66..806
                     /locus_tag="VAH76_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO1384932.1"
                     /translation="MPFVGEYRNFHLNMMMNTMKDEKIPATGFIIAKEVRPDNWEILH
                     KFRDAGFGLGNHTFSHANLNKLKVKEYIQEIKEADALLTPVLTEPKYFRYPYLAMSSG
                     DKKNKILCYLEKHHYRVAPITIDSKDFVFNQRLLSVPELGRRAYLDELKPFYLDFIWQ
                     QTLKAEEHTHYHRNPDQAQILLIHANLLNAYVLPDIINLYKQNGYTFVNLEDALKTFK
                     DNYHCHKTSKKTVEEEMDLNIESFMDWD"
     gene            1009..1722
                     /locus_tag="VAH76_000274"
     CDS             1009..1722
                     /locus_tag="VAH76_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEO1384933.1"
                     /translation="MNKVIRESQKFTLCNGAVLLFCMDTSVLPTQSNSNIAQNIQTRG
                     IIYNDLCEDSFDTKVPLNTNPLSIAGFTLVELLVTLSVFAIILTLIVPSLRTMILNNR
                     LTSNLDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTSCGANWGSGWIVVTQPVAGTP
                     TLLKTHQTSVNDPVITSNVSSVVFDPHGLSTTQSNFTICDNRGNAFARSAMVLATGFV
                     QSGTTPGQAVWNNGALNCP"
     gene            1735..2274
                     /gene="pilV"
                     /locus_tag="VAH76_000275"
     CDS             1735..2274
                     /gene="pilV"
                     /locus_tag="VAH76_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEO1384934.1"
                     /translation="MMNRMGTLAKYKGMTLLEVLISVVILAVGMLGIASMLIVSNKAN
                     NSSYAKLQAVQAVYDIFDRIRANSEAASNGSYNISNIGSNGYPTTVNTPSVLCNASVC
                     NPSQLAQYDTWYWLTRVVSQLPNGSGSITSALSGVAGNTVITVTVQWDDSKAQNELGA
                     SSASSGTANFVQFRVQSQI"
     gene            2271..3338
                     /locus_tag="VAH76_000276"
     CDS             2271..3338
                     /locus_tag="VAH76_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEO1384935.1"
                     /translation="MNINYRLQQGFSLVELMIATLVGLILTYSILQIYLAQTQLYKAS
                     NSQDLIQSTENAIANLLIPTIRSAGFAGCGSIITALSSLNAGGPRPLGVLNTTPTILM
                     GYNGSGASFTITQSNPANSTNASHWSPALDSTLVGNAQQGSDVLVVLGSTSDSYPTGI
                     TTIDPGSNTFVVQGTSGMSLTSGMFGSVSDCGKTVIFQITGVAGTTITHDAGAGVLQN
                     ASSAFPVNFQTGSQFIPIQQTAFFVGQGQGGQSALMRASLVGNNWTIQPLVPGVEIMK
                     VQYGIGNNGVITRYVSANAVTDWAQVYAIRLGFLIAGQLGSGSLTTRQYNVLDTQITV
                     PADNRLRHVFELTINLRNSIS"
     gene            3335..3847
                     /locus_tag="VAH76_000277"
     CDS             3335..3847
                     /locus_tag="VAH76_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEO1384936.1"
                     /translation="MNITPTNQYSKQRGYVLITSLVLMSMLTVLALTQISQNTSQTRI
                     ATNATDSEISFEKTEGAINEAINLIINGTYNPQSFLASNNGMYLFDQSATTPLWKTIN
                     WSSGSAVINSFQGNTGAQASYFLEKLPSVIMPGQSMKKPSNVYRITARSAGASGDTSV
                     LIQSTIQIQQ"
     gene            3860..7369
                     /locus_tag="VAH76_000278"
     CDS             3860..7369
                     /locus_tag="VAH76_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilY"
                     /protein_id="HEO1384937.1"
                     /translation="MDALKFWNLTRKGSQKAIKVLPRTLMAILLSASVSLYAVPLAIP
                     QIPLIIASPVHPQVLILLSNSQSMDGNLSGAIMTGSGSLSTGLSSLYNSSSPVNYTVP
                     AGFTPPVQGPDANGLAPYTVVQGGNKVDNSASRLNVAKAGIKAIIENYMPTTDFALGT
                     YSTSNISSYNTWVYYMSPPGSDFVFTNTPVAGNRYVTNPCYNYGSASSTVSSNCSSIG
                     NLYGTTLVSSSQYLQIGDSSDDPDINDALYAGSGFPGVFVSYNGPTPSSPFPPNYTIS
                     NYNQGNIRISYANTSPSIGNFSSSPTNAGFVPFSQQVMYVQRGFGYYSNQSYATGNML
                     VNMQTAGTNPTTTSVNNAINAFLPHLKPETNSTATTEIKAAAVQSPLAGLLTRSRSFM
                     KTVGTTSGNCPQKQYIILISDGLPTQDLQSRYWPPLGSAAATGYGVTATFNADGSLNS
                     TNSQALSDAINEIKALKNDGVLTFIIGMGAGVDPAVNPEAAATLRAMAVAGGTENYYP
                     ATSPEALVSSLNSILSNIQNGSFSTSAAAISSTRIDSNTVEYQANFITRDTPYQDWTG
                     NLTAIELDENTGQPTNIIQWSAQSTLDSLVSGTGWSTNRKIATWDPIAADGIPFRWAN
                     INATQQAQLQPSDLLGEKRLEYLRGNAALEKRNGGTFRDRSHALGDIIDSQVIYVGPP
                     SAPYLTASYISFAKANANRQPMLYVGANDGMLHAFNATTGEEFFAFIPNAVFNNLHQL
                     TSTLYNQNHLFFVNGSPESADVQFSDGSWHTILVGGENAGGKSIFALDITNPTNLSSE
                     PGVANAVLWEFTDSDLGLTYSKPQIGQIRSGSPSSLSFAVFFGNGYNNTNNKSVFYAV
                     DPQTGAVLRKIDLCAAVPSACNVNLPQGLSTVALGQKDGLQADPVTVVYAGDLQGNLW
                     AIDVSSADPASWSARVLFQARDSAGTPQPITTPPLVTLNPNYPRNQGLFVVFGTGQLL
                     TTSDLVTTQRQTIYGVWDKPLSSVPYVRANLQQQTLTLVNSATSGLSTNIITATANTI
                     NWSNKVGWFADLPIAGQRLITTPELINGAFIATINTPPLNSCGFGFTSMLLELNFLNG
                     GAFQYARFDISGDGGFDIADQYNGAYPVGIGLSNSYANSPTVLGPNKDNNLVILITQS
                     DGTQTTVIAPNITPRKIGWWQIQ"
     gene            7381..7830
                     /locus_tag="VAH76_000279"
     CDS             7381..7830
                     /locus_tag="VAH76_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO1384938.1"
                     /translation="MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQY
                     IQKSRRADAHATLTQDQIILERCYSQNFSYAAACGALPAFPQTTPNGYYTINISNLTA
                     TTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG"
     gene            7908..10115
                     /locus_tag="VAH76_000280"
     CDS             7908..10115
                     /locus_tag="VAH76_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO1384939.1"
                     /translation="MEIFCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGC
                     GFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQRPNKTQFHFAIEKINNNPAQGL
                     IQLNWYNKPPVLRAGQRWQLSVKLKKPRNFRNPGGFNYVRYLAARHIHWTGYIRYGNN
                     KLISNQPPSFSWLTLREKIGDALTKLAPNTETAGIIEALTLNVTHHISSEHWNLFRRT
                     GTVHLFGISGEHIALVSGIFFIVIRWIWSMSYRCCLFLPAHTIASIGGLLAALPYAFL
                     AGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIEPHAVFLQGFYFSFL
                     AVACLLLTHQRWPLKNYKKTLTLQLSCLIGLMPLTLHWYSYGSINGFLANLFSIPLVS
                     FLIVPLALMTMITSPLHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMM
                     LAMTAGLFLRVLLPVKPFKHLAILWIILPLFPPRTAIYPGEALINILDVGQGLAVAIQ
                     TQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGGLNSLEK
                     EMQVNQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIKESFTKKNNNSCILQVSNS
                     AGRVLLTGDIEKAAEEYLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVNPLYAIAS
                     LGFDNRFKFPHSKTLQSMKTLDITFFRTDECGMAAIKLPASGQVHRPSCMQAN"
     gene            complement(10171..11478)
                     /locus_tag="VAH76_000281"
     CDS             complement(10171..11478)
                     /locus_tag="VAH76_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO1384940.1"
                     /translation="MHTQRGLEVTTGSIEAVDSINYFHEQVLSSGQNAIQILDSAKKH
                     TDNLLIQTYAAAFYLYAQEDVANEQASDYLQSASRLLTSANEREKLTFEAVNNWSKRD
                     YANAISLLVTLLERYPRDTLALKFVEWLFYCTGQAFNAEYFLKVCDKCAPENQDESHF
                     LAIHSFALELCGQYSKAREMAEEAITMNLHTPWAHHTLAHVHLLTSDITGGINRLRDL
                     QKTWEDILPLLKGHNTWHLALFYLANRDEEEVKKLYPHISGALPDTVLEQLDTISLLW
                     RMDMAGLPQDRLLNQVVDHLSTHPLEFYTGFTNAHFIYCLVKSGYKNEADESLKRMKS
                     YACAPSSDALWGDVVLPLCQGIYAFADADYKTALMLMEPVIGECAQLGGSDAQIELFF
                     QTYLLVLIHTNQKDKALQFFTEHLKYYNNTPLSDWWFQSANKT"
     gene            complement(11679..12056)
                     /locus_tag="VAH76_000282"
     CDS             complement(11679..12056)
                     /locus_tag="VAH76_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384941.1"
                     /translation="MMKKINLFKLLMIFVLGTSALQAAGLSTQRIEQLSNARVTVWKT
                     IIYPSSKQVLPMHRHDNDRVLVALTDGTLKITNNKGKVHYLKLKKGKSYYLTKDVPNE
                     LHNDENVTNHSIQVMVIQLNDKA"
     gene            12193..12585
                     /locus_tag="VAH76_000283"
     CDS             12193..12585
                     /locus_tag="VAH76_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384942.1"
                     /translation="MLTTFLATVIGWYLVIVSLFLLFHNEHAKSAMAEIIAHRGSQLI
                     MAIVTLITGLLLVVSHNIWVMGWPVVITLFAWLVLLGGLIRFLCPDTAVRVGQSFVDN
                     PMRLKIAAVIFFIIGLFLLFNVYYVYLK"
     gene            12711..14639
                     /locus_tag="VAH76_000284"
     CDS             12711..14639
                     /locus_tag="VAH76_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384943.1"
                     /translation="MTRAQTHPHQTTVISNLKIIPLTLGWLLKFALLIIIIAVFTPLS
                     PKMPAPGLDASWAVGLNQAVAQGLAFGKDIFFTLGPYSSIYTKFYHPATNFMMVGGSL
                     YLALCYWIAIILLMQGIKWRWSIAFAILLFSMIYARDSLFFSYSLIIGLLIHKEIPGK
                     ETSLKSNFFTLFALLFTPFGLFPLIKGSLLILSIITILLCSFFLSLYKQRNLVVLCLA
                     SPVVSILFFWIASGQSVFNLPAYISNTISLASGFTEAMATEGDNKELIFFGITSSCIL
                     LSILLQKQLLPLSKAFLFCIYFVFLFLSYKTGFTRHFGHAFIAGTSVLLASFLLPYFN
                     SNNKVIVPLLALSLYTANYINGHYTKISVRDNFISTYTAAYYGLQNRIQNKNWLKENF
                     SLTMNYLKARDDFPRLPGTTDIYSYNQTSLIASGMQWSPRPIFQSYSVFTAKMAEINT
                     NYLLSENKPDNIIFKVEPIDNRIPSMEDGMSWPTLINHYQPVRLENDFLFLRKKLVNK
                     PVTSLVPLKTEIHTFGEQIKIPDTDKPLFVEIEIQPTLWGRLATTFFKPHQLEANLEL
                     KNGTQRQYRLISNMAKSGFLLSPLIENSAEFGLLFSQNNYLAGKKVISFSINSKQVPS
                     SHWQTKYTIHYKIFASNN"
     gene            14799..16223
                     /gene="sidA"
                     /locus_tag="VAH76_000285"
     CDS             14799..16223
                     /gene="sidA"
                     /locus_tag="VAH76_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector SidA"
                     /protein_id="HEO1384944.1"
                     /translation="MAKKYRTPIPLSDKMMTLKKNLQDQLTQSQSLGEQIHAGTTAAS
                     TITTGVSHVFNVIDSFLSAYRQIPLVGGILQLVATVAKSISIVSDPEKSIAEKIISSI
                     LIAAIIALSITAITLGGLAAAIIGTVIASTVTIMEGLGFFGKIAEKFQLANSYKMKKE
                     FIDLVAKRIEPDNDKYNDLLEIRAIELQHELEKSYPNQIEQQQLKEELNFINTVLRKK
                     NITPGNNKENDAYKLNQLYAKRAEQLKTLVDKIALINPTGKRAEQNKLLSEIKSLQKE
                     ITKTDDSIEEIIAPLAKIERDNLKSTINLPLSYSTFAIAAASTLISIAGLLVVAGVIA
                     APPIIVPIMFAVGSVMAIIGLIKWTAEKMAEIEDAQLKAEETAKHEDAVLDEALNVYE
                     QQLNLELVSPANPARQVKNILTVQDKVPALSPTESTVSSLDSRFTLFGSSSREHTKIA
                     EQKTNDTTNEQAPTTFDSISKPTR"
     gene            16334..17287
                     /locus_tag="VAH76_000286"
     CDS             16334..17287
                     /locus_tag="VAH76_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HEO1384945.1"
                     /translation="MARNKRLDRFHNAQNSNTCGYDQAFSEIQGGRKRSHWIWYIFPQ
                     LENLGKSPSAKLYGIKDFNEACDYLKDPVLFKRYFELIKLIEEKLKNIPLPHLMGGFP
                     DDRKFISSLTLFQATSNYLSSKDTDSQNKYHELYETCNRVFKQIAPQGYSPCQQTELL
                     VKHQSNSPVKKTTEKSSQPPQVTIFSPKRSKSNTAHPILKDLAQYINKRGNEWSYHYN
                     FLGLVAFVYFIQDLILGTDYLNDKARQVKLTAASKLQKILDPQCHERCNLNPSERKAL
                     SDGRLGEIINKHGGLDKILTEAPRKPPKTGHDQEINISPFS"
     gene            17560..18132
                     /locus_tag="VAH76_000287"
     CDS             17560..18132
                     /locus_tag="VAH76_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO1384946.1"
                     /translation="MSQNIILNRCEWVGQNKPHYELYHDTEWGIPVHDDEKHFEMLIL
                     EGAQAGLNWETILKKRDGYRRAFKQFNPQAVAQMTDDELNALLINPEIIRNRLKIFST
                     RKNAKVFLSIQQEYGSFDSYVWQFVNGAPILNRPESIRAIPATSKESDALSKDLKKRG
                     MSFVGSTIIYAYMQAIGMVNDHIVTCFRAK"
     gene            complement(18292..19383)
                     /locus_tag="VAH76_000288"
     CDS             complement(18292..19383)
                     /locus_tag="VAH76_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384947.1"
                     /translation="MKKYHQLLMGITLLLFGVCNYAGDTGPVENSDLFRVFIPNLKPG
                     YEFSATAFYLQPGADNLGWGVITTVLPIPSPNWQVVTFNPDYQVGFNLGGRYVFANSG
                     SDLQLNWSHLRTNDTDNVFVNPSSQWISPFSQTGTPPTPSGQITGVALLKLAHASLDF
                     SYDAVSLDVGKFINFGSKLQTRLFTGLSSAWIKEELISTFRGFSLPILSLNNTSTYKG
                     VGPRLGFNNSYNISHGFNLVGQLAGSVLYGRMQPAQYQFTGTSQQLIIAGIFVNREGL
                     ANPSVNQLVPALDAKIGLSYLYSLKQNWDLNFEAGYMGALYFNPLSSYETNTNVIALD
                     TGSLSTSSAKHTQSNFSVGGPYITVSLRT"
     gene            complement(19776..20633)
                     /gene="queF"
                     /locus_tag="VAH76_000289"
     CDS             complement(19776..20633)
                     /gene="queF"
                     /locus_tag="VAH76_000289"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEO1384948.1"
                     /translation="MNKELEIIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQE
                     INLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNS
                     LNNTNFPDVETVVQTISKDLSHCIDSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIE
                     CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIVYKGKKINHEGLLK
                     YLISFRNHNEFHEQCIERIFADIMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFD
                     GQNIRLIRQ"
     gene            20779..21180
                     /locus_tag="VAH76_000290"
     CDS             20779..21180
                     /locus_tag="VAH76_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO1384949.1"
                     /translation="MINQQIEVFLKSPAFAVVGASSNREKYGNKVLRCYLRNGKKVYP
                     VNPHETLIESLPVIHSVNDLPDDVHSISIITPPSITEEVVKEAVKKGIQHIWMQPGAE
                     SDSAIQYGLDHKLNVISGGPCILVVMGYEEN"
     gene            complement(21245..22366)
                     /locus_tag="VAH76_000291"
     CDS             complement(21245..22366)
                     /locus_tag="VAH76_000291"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEO1384950.1"
                     /translation="MPLQPFKTSNLLTMGVELELQLISLSNFDLTAASPDILELLGRS
                     SFPGSFTPEITESMLEIATDVHEEYDQLLKQLFHIRDTLVTVGDRLNIGICGGGTHPF
                     QMWSDQRIFNKTRFIEVSELYGYLTKQFTIFGQHIHIGCEDGNQALFLLHSLNRYIPH
                     FIALSASSPFVQSKDTLYNSARLNSVFAFPLSGRAPFVLNWDEFSLGYFEKMEHTGIV
                     KSMKDFYWDLRPKPEFGTIEMRVCDSPLTVERAAALACYMQALCSYLLENKEPLPHED
                     DYLVYNYNRFQACRFGLDGTLVHPKTYEQILLREDILTTLRRLKPYANQLNSTMALEH
                     IYEITHKGSDASFLREKYAEHRTLESVVNESLKQFRRSK"
     gene            complement(22368..23555)
                     /locus_tag="VAH76_000292"
     CDS             complement(22368..23555)
                     /locus_tag="VAH76_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1384951.1"
                     /translation="MIDVGKIITDLAWPISLLIAWFAGELGYRWAKLPHISIYAIVGF
                     SLASTQIGLLPSIQPPSILLLANIGFGLILFESGYRINLRWLSYNPWVAASSITEATL
                     TFCLVYILVKSFGLQTSTCLLLASLSMATSPATVVRVINEQRSSGQVTERVLHLSVIN
                     CVLAVFLFKVIIGLVVFRTSGNLWEAAYASLTVTAISTIWGIIFGVIMPILLKTVKST
                     CQDNTLAFACAVILLVILTHNLKLSPVLATLTFGLVSRHGRMVFSPSQPGFGTLGDLL
                     SVLLFVFIAATIEWQRVVTGVWIGLSIITVRFVAKIIGISLFAHISGISFKKGILTGL
                     AMTPISVFVILILEQSRYIGINLVDTLAPLAMVALTLEILGPIMVQRALIKANEISAI
                     KEF"
     gene            23885..24487
                     /gene="def"
                     /locus_tag="VAH76_000293"
     CDS             23885..24487
                     /gene="def"
                     /locus_tag="VAH76_000293"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO1384952.1"
                     /translation="MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLY
                     ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIFPMHILINPNYEPLPHTRIISD
                     FEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
                     RLTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK"
     gene            complement(24523..24927)
                     /locus_tag="VAH76_000294"
     CDS             complement(24523..24927)
                     /locus_tag="VAH76_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEO1384953.1"
                     /translation="MHKLIELQGVGPKYAEMLKRVGIESQDDLLRVCSGRRGRFRVAE
                     QTGISHKLILKWTNQADLARVNGINEEYAELLEQAGIDSIPELAHCNPEQLYSSLKEK
                     NEQCHLAQHVPGLSQVVTWVEQAKKLPRAVYH"
     gene            25115..25459
                     /locus_tag="VAH76_000295"
     CDS             25115..25459
                     /locus_tag="VAH76_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384954.1"
                     /translation="MRKHKNKMVRIFLLALGLFSSTTINAWYGSGITIGVARANVHYY
                     GDDIRVYDPWGYGYYDRNYYYNGVRNIIVPNVIVPNVVINVPVQRYYVPSCEEMEVCD
                     PYGQCWLEQFCR"
     gene            25660..26706
                     /locus_tag="VAH76_000296"
     CDS             25660..26706
                     /locus_tag="VAH76_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEO1384955.1"
                     /translation="MSETIGYAAHKAGAPLKPFTFERRPVGAHDVAIDILFCGICHSD
                     LHQVNNDWAGAFYPMVPGHEIIGRVKEVGHQVRRFKAGDLVGVGCMVDSCRQCTYCHQ
                     NEEQYCKSGATFTYNSQDNHLGGVTYGGYSKHIVVTEDFVLKIDESLDPAAAAPLLCA
                     GITTYSPLKRAKVGKMSKVGIVGLGGLGHMAVKLARAMGAYVTVFTTSINKIKAGKDL
                     GAHDVVLSTDSNVMQTLANQYDFILDTVSAKHDLGQYLNLLKVDGQLTQVGLPSDPLE
                     VTMFPVILKRLSINGSLIGGIKETQEVLDFCARHGITADIELIKMHEVNSAYERLLKG
                     DVRYRFVIDMSTLD"
     gene            complement(26760..28079)
                     /locus_tag="VAH76_000297"
     CDS             complement(26760..28079)
                     /locus_tag="VAH76_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO1384956.1"
                     /translation="MYNILLIFIAFLLVLLNAFFVSAEFGMVKLRATRIQAIKENYGL
                     RGRILEQVHQNLDAYLSACQLGITLASLGLGWIGEPAFAELLGPLFVALGISSVHIVT
                     ISSFFIAFFLISFLHIVIGELMPKSIAIRQSELISIWTAVPLYWFYWIMYPAIWLLNT
                     CSNFFLKLSGMDIKNEYEGFYSSEELKLILSGQHLQSELKEDEREILEHTLDLAELNV
                     TEVMRPEDEMIIIDINEPFETIKDKILKYRYSRYPVYDSGTKEMIGVIHVKDLFADLL
                     LQKNINTLKPYVRPVLKVSHKLPAMDLLRKFRAGMPHFALVYKHKSSLLGFVTLDNLL
                     HILIGRIKDEFHKTHEDWVKNSDGTITTKGDCSLYSLERALDCDITLSPDEEELNTLY
                     GLIITRHGKLPNEGEIIQFVEFDALIEKVQDSFITKIKVFPKIKNQE"
     gene            28733..30028
                     /locus_tag="VAH76_000298"
     CDS             28733..30028
                     /locus_tag="VAH76_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1384957.1"
                     /translation="MNQNSFKPKNNFVSLVMWALPLSFFTFQFLLRLWPGLTMQDIMA
                     QFSIDAASFGLIAAFYYYGYAGMQIPVALLLDKFGARFVVFAFAMLCGIGTLVFIGTN
                     NFYLALSGRFLIGVGSAAGFLGVSKVVSEWFSHSQYSRMIGFSFSVGLLGAIYGGKPL
                     AHLIQNFSGQQVGLALAVFSILLGCCALLILRSPPNNTHSVTKTMNLSHCSSLFSSWI
                     IWALALANLLMVGALEGFADVWGVPYLIAKYALNKTDAAFLISFIFMGMIFGGPLLAF
                     LSQKLGNYFVIALCGTGLAFLFLILLYTATLHWWQLGTLFFAIGLLCCYQVIVFAAGS
                     TLVPYEGLGVTIAFLNCINMLGGSFFHTLIGKLMDSSWSGQMSANGLKLYEVESYQWA
                     LSLIPFCALCGSLLIVLIHIKTRAYAAPELMKKTSLTGV"
     gene            30031..30675
                     /locus_tag="VAH76_000299"
     CDS             30031..30675
                     /locus_tag="VAH76_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEO1384958.1"
                     /translation="MEKIFWSNPYQHTLDTKIISINGNEILLEKTIAFSFSGGQESDR
                     AFINGLEVLSSRIDGNLIYYTLPDDHNLAPNDIITMTIDWPRRHRLMRLHFAAELILE
                     LVTRTSMLEKVGAHISESKARIDFKANFNISSIFSQLLDEYNAIIRQDLVIHKGYHDV
                     TNQRRFWKIDGFAEVPCGGTHVHSTSEVGFIRLKRSHPGKAIERIEITLMDDSR"
     gene            30922..31836
                     /locus_tag="VAH76_000300"
     CDS             30922..31836
                     /locus_tag="VAH76_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEO1384959.1"
                     /translation="MELPYHFSTVYVSKPEFIEALSEELGEVSNKIGSLVFSPHQKLD
                     VCFALDVWLEPEIVTIQSISEAAKILRQAGKFWYLHPVTEIRRSRLIEEQLRKCPSLM
                     SHFPVTSDIPPIGVFSLLDRNTLLFSAKRLKKWPDGQCYFIEDKVNPPNRAYLKLWEA
                     LFLLNKYPLPGETVLDLGASPGGWTYVMQSLGTQVTSVDKAELDTKIMALPRVSFLKQ
                     SAFALDPEQLTESYDWVLSDVACYPDRAYTLIKKWIASKKAKQLIFTIKLQGQTDLNT
                     IKRFQSIPNSKVINLFYNKHEVTFFYPV"
     gene            complement(31908..32861)
                     /gene="epmA"
                     /locus_tag="VAH76_000301"
     CDS             complement(31908..32861)
                     /gene="epmA"
                     /locus_tag="VAH76_000301"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HEO1384960.1"
                     /translation="MSDNSWLPSASIQLLRARAELISKIRQFFTERQYLEVETPVMAR
                     HGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHN
                     PEFTLLEWYQLHIDHHVLMDEVDLLLQIILKSRPMIRKTYQQAFEEACGINPFSTSIE
                     QFNKVLAQHDLDGVLSPNEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALA
                     QVKEGLAERFEVYYQGVELANGFHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLL
                     KALEYGLPPCSGVALGVDRLLALALKQPNIANVFSFDFSRS"
     gene            complement(32864..33199)
                     /locus_tag="VAH76_000302"
     CDS             complement(32864..33199)
                     /locus_tag="VAH76_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEO1384961.1"
                     /translation="MFGFKKKQSNESIKEGVITALRGVFDPEIPVNIYDLGLIYDIAV
                     NDEGHVHIQMTLTTPGCPVAQTFPGTVEQAVNQVEGVSDCTVELVWEPPWSQDRMTEA
                     ARLELGIFY"
     gene            complement(33271..33720)
                     /locus_tag="VAH76_000303"
     CDS             complement(33271..33720)
                     /locus_tag="VAH76_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO1384962.1"
                     /translation="MSMELRELYQEIIIDHNRNPRNHHAMEDATTEAKGFNPLCGDKL
                     TVYLKLQGDLIRDVSFVGCGCAISQASASLMTDALKGKSIKEAHELFHRVHRMLTQEE
                     EDSLVSMDKLTVLAGVKAFPARVKCATLAWHTLEAALNKETEVVKTE"
     gene            complement(33717..34961)
                     /locus_tag="VAH76_000304"
     CDS             complement(33717..34961)
                     /locus_tag="VAH76_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEO1384963.1"
                     /translation="MSTNPSLMTSFDVNKIRKDFPVLHQKINEYDLVYFDNAATTQKP
                     KAVIDAIAQFYEKDNSNVHRGVHALSVRATEMYEAARAKVKRFINARSPRECIFVRGT
                     TEAINLVAQSLVAPRILPDEEILITHMEHHSNIVPWQMVCKKMGCKLQVAPISLNGEV
                     ILEEFERKLNENTKMVAINYASNSLGTINPVKTMIKMAHEVGAKVLLDGAQATAHLIV
                     DVQDLDCDFYAFSGHKMYGPTGIGVLWGKEELLNSMTPYQGGGEMINSVSFEATEYAA
                     IPHKFEAGTPNIAGAIGLAAAIDYIWSLDLDAIAEYETQLLNYATKAIEAVKGYNIIG
                     TAANKVPIISFVHGKIHAHDIGTILDSEGIAIRSGHHCTMPLMDFYDVAATSRISMSF
                     YNTFKEIDYCMEALQRVKEVFA"
     gene            complement(34951..36237)
                     /gene="sufD"
                     /locus_tag="VAH76_000305"
     CDS             complement(34951..36237)
                     /gene="sufD"
                     /locus_tag="VAH76_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO1384964.1"
                     /translation="MSDILDYYQQQAQSGVSQIPWLAQLQSKALTDLKRRGFPTRYDE
                     EWKYTLVDSLLQQHFVLPDPTVKPALKTAFDDSPVKNLFLIQNGFISQEQEWIKNLPE
                     GVLILPLSLALIHYADLVKPYLGKIVEQEHGFHYLNTAMLQCGVFIYIPAGICLEEPI
                     VLKHIQDQTNQAMHLRHLILMEKESQATLIEEYQGEENICYFTNSITEVFLGAGAKLN
                     HYKIQKESKSAYHLGHLSVRQSAASQFSSHSLSLGGKLVRSDISVSMQEEQAQCLMNG
                     IYAPGDGQHIDHHTVVHHLVPNCRSDQDYKGILKGHSRAVFNGKVIVAKNAQHTDAKQ
                     QNKNLLLSANAEIDTKPQLEIFADDVLCSHGATVGQLDEEAIFYLATRGISRDEASHY
                     LIQAFANDNLRLIPNRDLADWMGNLLTQQLGYANEY"
     gene            complement(36234..36986)
                     /gene="sufC"
                     /locus_tag="VAH76_000306"
     CDS             complement(36234..36986)
                     /gene="sufC"
                     /locus_tag="VAH76_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO1384965.1"
                     /translation="MLNIKELNVAVNGQSILKGINLEVKPGEVHAIMGPNGSGKSTLS
                     KVLAGHPSYEVTRGDISYLGQDLLPMSPEERARAGIFMSFQYPVEIPGVTNINFLKAS
                     VNAVRKGQGRNTLDAIEFLSFIREKCQLLDMDESFLYRSINEGFSGGEKKRNEILQMA
                     ALEPRLAILDETDSGLDIDALRIISQGVNAMRSPERSIILVTHYQRLLDYIEPDFIHV
                     LANGRIITSGDKSLALELEKKGYSWLEEVEQL"
     gene            complement(36988..38436)
                     /gene="sufB"
                     /locus_tag="VAH76_000307"
     CDS             complement(36988..38436)
                     /gene="sufB"
                     /locus_tag="VAH76_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO1384966.1"
                     /translation="MAKSSEQLNSLLDREYQHGFITDIEVETFPPGLDEDVIRRLSAI
                     KGEPEFLLEWRLKAFRHWLTMPHPEWSSVHYPPIDYQSVSYYSAPKSKKDAPKSLDEV
                     DPELLKTYEKLGIPLREQEMLAGVAVDAVFDSVSVATTFKAKLAEKGVIFCPLSEAVH
                     QYPDLVKKYLGSVVPYRDNFYAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINAAST
                     GQFERTLIIADVDSYVSYLEGCTAPMRDENQLHAAVVELIALDGAQIKYSTVQNWYPG
                     DKEGKGGIYNFVTKRGACRGKKSKISWTQIETGSAITWKYPSVILQGDDSVGEFYSVA
                     LTNNFQQADTGTKMIHLGKNTRSTIISKGISAGHAHNAYRGLVRIAPTATNARNYTQC
                     DSMLMGSQCSAHTFPYIEVKNPTAQVEHEATTSKISEEQLFYCQQRGIDTEDAVSMIV
                     NGFCKQVLKELPMEFAVEATKLLGISLEGAVG"
     gene            complement(38429..38890)
                     /locus_tag="VAH76_000308"
     CDS             complement(38429..38890)
                     /locus_tag="VAH76_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEO1384967.1"
                     /translation="MLRVSKLADYGTVVMVYLAKHSQELCNARDIALHTHLTVPTVSK
                     ILKRLTSAGLLTSVRGVSGGYRLQRPAAEISVSQIIFALEEHRGFTECSMQPNDCFLQ
                     GVCTIQGNWRLISHAIETALDSVSLEALAKPTLPTRDVERVRQLASGVNRG"
     gene            39331..41073
                     /gene="phaC"
                     /locus_tag="VAH76_000309"
     CDS             39331..41073
                     /gene="phaC"
                     /locus_tag="VAH76_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEO1384968.1"
                     /translation="MTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTE
                     HFQNLIAVILKNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWIN
                     NPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQ
                     LMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRN
                     SMMELIQYCPRTTKVKSIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWV
                     NPDETYANKSFYDYLNEGPREAISVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATK
                     DLSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRA
                     NDLIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNDLIKPGKIR
                     LNHTPIDVTNIDIPTFFLSTQKDHIAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINP
                     PTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGRLINSPDINHLPF
                     APIMDAPGSYVLKK"
     gene            41122..41505
                     /locus_tag="VAH76_000310"
     CDS             41122..41505
                     /locus_tag="VAH76_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384969.1"
                     /translation="MRKKNRILLASLFSLGSLNAFAAQDLTVVNTSDDLGYVTIGSYS
                     CELTSSCAIPPDNSIRINSDIVEAACANTPADCEIVFGVDSIKARIATGSFSLNEGIQ
                     YIAQSRWLNYSAKRLSKTVAEIYKR"
     gene            complement(41592..42011)
                     /locus_tag="VAH76_000311"
     CDS             complement(41592..42011)
                     /locus_tag="VAH76_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEO1384970.1"
                     /translation="MKLIWIVLALILALVVILYLFPQINFREMPKGLVEGQLPDDKPN
                     WVSSLVNESDSHYIAPLKVESMPQLVACIERRIPELKIQKQEASFLVGYRQSPFFHFV
                     DWICIHADGNVISSATMGYSDFGKNRELVEKIRSLCL"
     gene            complement(42012..44213)
                     /locus_tag="VAH76_000312"
     CDS             complement(42012..44213)
                     /locus_tag="VAH76_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO1384971.1"
                     /translation="MMTKTNEENLTWKGKQKQRARRWIKIFLEKWFRIEVKGQYQANS
                     HSVIIANRASIIDVLLLSVFLPERLTLALHPDMFKKIWVKTLLFFADVIVVDPGSAQA
                     TRILIRAIRAGKRCLLFPQGLLRQQEDSLKAFAGPGLILQKVGAEVIPVRIEGAEHSI
                     FSNSKNKYLIRIFPKITLHILPAQKLSQSENTGVDREGISLRLFQLISDLSFANSFKP
                     YVLFSALIEGVSIGTKNKTKIEDNNRIPLTYRQFLTRCFILGRQIKKQTAIGETVGVM
                     MPTTIAGMVTFFALQAYRRIPAMLNFSMGFYNLYSACNTARIKSIYTARQFIETAKLE
                     PLVEELQQAGIRIHYLEDFKSTIHLGNKLSGIFKGFFPTLSYRFLGDKISPDTTGIIL
                     FTSGSEGKPKGVALSHANILANCWQMTSRVDFTPRDVLFNSLPIFHCFGLTAGSVLPL
                     VNGLNCFFYPSPLHYKVIPGLVYQTGATILFGTDTFLTGYARAAGKHDFNSVRYIFAG
                     AEKVKPETIRYWSEAFGARIYEGYGATEASPVISLNCPLASVPGSVGMILPFMDFKVE
                     PVEGIVQGGRLKLCGPNIMLGYLDEDKPGVIIEPEDGWHDTGDIVTVNADGFITIAGR
                     AKRFAKIAGEMVSLTAVEGIAASIWPELLHAAVVQKSPRKGEQIFLFTEAEYADKVSF
                     IKKVKERGQSELLVPHMIYPACQIPVLPSGKIDYITLEKQFLEQKETMETV"
     gene            44368..45051
                     /locus_tag="VAH76_000313"
     CDS             44368..45051
                     /locus_tag="VAH76_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO1384972.1"
                     /translation="MRVLNSCNKVRGIFTLTQKTIKTFCFILAILVLIIFAVVFHNHS
                     LEIIDYIDDLGWLAPVLFLITYCLATLLLLPTMVLTLAGGAVFGPVFGTLLNLLGATS
                     GAAFAFLITRHLVYDWFSTKKGEKLNKLIAGVDEKGWVFVAFLRLFPIVPFNLVNYGL
                     GVTGISFRLYLLTTFIFLIPAEIIYTYFGYVGMDALTRPGHLYRNGGVFLTGLAIFFL
                     LVIRIMKKR"
     gene            complement(45078..45893)
                     /locus_tag="VAH76_000314"
     CDS             complement(45078..45893)
                     /locus_tag="VAH76_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEO1384973.1"
                     /translation="MPTRVIEDKGDMTPSFGIDDRIFLGEGLFETIRVNSSKPSFAYM
                     HWERLGNSARQLGIPFEISFDDWFEHLIQKIQKDNLYHGGIKAILSGGPASRGLAERG
                     QVSQLIFQTFNYSIQKHPVRLISINWLRDKANPLYQLKSVNYLEAIIAQRQAIAVGAD
                     DALFFNTENHVTETTCANLFLIENNILYTPRIEDGILPGITRARLISHCQQHKMSVQE
                     ISLTKKRIEDADAVFLTNSLQGIRRVLSLDNIIFEVNHPIIDKLIFLLNQDES"
     gene            complement(45907..46092)
                     /locus_tag="VAH76_000315"
     CDS             complement(45907..46092)
                     /locus_tag="VAH76_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384974.1"
                     /translation="MSDLFEEDEEEVIEVVDDFDDVEVDTEIIEAPGVSLDARRRLEN
                     MLEEKRLRDELDDFVDY"
     gene            46339..46920
                     /locus_tag="VAH76_000316"
     CDS             46339..46920
                     /locus_tag="VAH76_000316"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO1384975.1"
                     /translation="MSDQYKVALRNTIKQVRSKLSVSYRTASSNQICTRIRSLEQYRQ
                     AKRIALYFAVNGEVDLTFLWKSAPLQGKYCYFPVLNNDSTLSFLPATPNTPFKTNKYD
                     ILEPDVDIELALPVDELDLIIMPLVAFDAHCTRLGMGSGYYDRTLANKSKAHLLGVAY
                     QFQRVDYIEPRPWDVPLDAVITQKAIYWRNPLA"
     gene            complement(46930..47268)
                     /locus_tag="VAH76_000317"
     CDS             complement(46930..47268)
                     /locus_tag="VAH76_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEO1384976.1"
                     /translation="MKMITAIIKPFKLDDVHEALMEIGVPGITISETRGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIELALPDNMVEAAIEAICKSAYTGKIGDGKIFVYALEQVVRV
                     RTGEVGTDAL"
     gene            47413..47667
                     /locus_tag="VAH76_000318"
     CDS             47413..47667
                     /locus_tag="VAH76_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEO1384977.1"
                     /translation="MFDPKQFDELANKLFATLPTSLQNFEKDIQQKFKEVLQSTFSRM
                     DLVTREEFDVQCKVLARTREKLEQLQHQLEDFIKRQEIKK"
     gene            47739..49250
                     /locus_tag="VAH76_000319"
     CDS             47739..49250
                     /locus_tag="VAH76_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEO1384978.1"
                     /translation="MNLAFTKTRSTIGIVAQPVSVEVHLSNGLPSFTMVGLAETAVKE
                     SKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSGFDLPIALGILAASEQIPLTNLA
                     NHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHPKVFTANNL
                     REVCDYLCQGTSLQNLPPKPDLLLNNHELDWSDIKGQHHAKNAMVIAACGGHSILLSG
                     APGSGKTMMAKRFSTLLPELSETQALECAAINSIRGKLPDFREWRLPPFRAPHHTASP
                     VALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLESGHICISRAAAQIE
                     FPAKFQLIAAMNPCPCGQWGNSQANCLCTPDRISRYLAKLSAPLLDRIDMQVTIHALS
                     QEELIKPNTHLEKQSPAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLF
                     LREVMSKLKLSARGYHRLLKVSRTIADMSECNKVMLNHLQQALSYKQILHLPK"
     gene            49354..49740
                     /locus_tag="VAH76_000320"
     CDS             49354..49740
                     /locus_tag="VAH76_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384979.1"
                     /translation="MRSIRLLFPCILLMIPWQFATANRPTDNALAIIKSCYDESVNLS
                     NDFTPCVTKKLKTMPNAEDFQLIMNFKTDEKGTNERIPVFMVDKTGYMYYCIVTTGKT
                     LIINACAGEQGKPLTQGQMLSIDLPE"
BASE COUNT        15507 a        10458 c         9195 g        14846 t
ORIGIN      
        1 ttttcctgat aggatttaat aatgtgtttg ctcaaaaaaa agccatagca atcactattg
       61 atgatttgcc ctttgttggt gaatacagaa actttcatct gaatatgatg atgaatacga
      121 tgaaagatga aaaaatcccg gcaacaggtt ttatcattgc caaagaagta agacctgata
      181 attgggaaat actgcataaa ttccgtgatg caggtttcgg tttgggcaac catacttttt
      241 ctcatgccaa tttaaacaaa cttaaagtaa aagaatatat ccaggaaatt aaggaagcag
      301 acgctctttt aacgcctgta cttactgagc ccaagtattt ccggtacccc tatttggcca
      361 tgagctctgg agataaaaaa aacaaaatcc tttgttatct tgaaaaacat cactatcgtg
      421 ttgctccaat tactattgac agcaaagact ttgtctttaa tcaacgctta ttatctgtgc
      481 ctgaactggg caggcgagct tacctggatg aattgaagcc attttatctt gattttatat
      541 ggcaacaaac tttaaaagca gaagagcata cacactatca tcgcaatcca gatcaagcgc
      601 aaatcctgct aattcatgcg aacttactca atgcctatgt cctgcctgat attattaatt
      661 tatacaaaca aaatggttat acttttgtca acctggaaga cgccctaaaa acattcaaag
      721 ataactatca ttgtcataaa acctccaaaa aaactgtcga agaggaaatg gatttaaaca
      781 tagaatcatt tatggattgg gattaataac cactgtgaag tcttcagact gtaaaaaatt
      841 ttttgccaaa cctcttactt tggctcctaa tgtcattcta tctgtttagc atcggtaaac
      901 gaaaacgact cttaaaattg ctaattgggg cgaatctgga aaaaaattaa ttaatttgtt
      961 ctataattaa caatgtaatg ggtttgatca aacgataggg ataatgcttt gaacaaagtg
     1021 ataagggaat cacaaaagtt cactctgtgc aatggcgctg ttttattatt ttgcatggac
     1081 accagcgtat tgcccacaca atctaattct aatatagcac aaaatataca aacaagaggc
     1141 ataatttaca atgatctttg tgaagacagc ttcgatacta aggtacctct taacactaac
     1201 cctctttcca tagcagggtt cacactggtt gaattattag taaccctatc tgtttttgcc
     1261 atcatcttaa ctctaattgt accttcatta agaacaatga ttttgaataa ccgtttgact
     1321 tcaaacctag attcactggt aagctcatta aattacgcgc gtggagtggc acttgatcgg
     1381 gcagtcaatg tggcagtatg tccgttaggt tcaccaggtt caacgagctg tggagctaac
     1441 tggggctcag gatggatagt agtgacccaa ccagtagcag gaacaccaac tcttttaaaa
     1501 acacatcaaa cctcagtgaa tgatcctgtt atcacttcga acgtatccag tgttgttttt
     1561 gatccacatg ggttatcaac aacccaaagt aacttcacta tatgtgataa ccgaggaaat
     1621 gcgtttgccc gatcagccat ggttttggca accggatttg tccaatctgg aacaacacca
     1681 ggccaggctg tctggaataa tggagctctg aactgtccct aacggagata gaatatgatg
     1741 aaccgaatgg gtacattagc aaaatataaa ggaatgactt tgctggaagt attgatttcc
     1801 gttgtcatat tagccgttgg catgttaggg attgccagca tgttgatagt cagcaacaaa
     1861 gcgaataatt ccagttatgc caaactgcaa gccgtacaag cggtctacga tatttttgac
     1921 agaattcggg cgaattcaga agcagcatca aatgggagtt ataatatcag taatattggc
     1981 agtaatggat accctacgac cgtaaatact ccttcggtat tatgcaatgc atcggtctgt
     2041 aatccaagtc aactggcaca atacgatacc tggtattggc ttaccagagt ggtgtcgcaa
     2101 ctgcccaatg gaagtggttc aataacctcc gctctttcgg gtgtggcggg gaataccgtg
     2161 attacggtca ctgtacaatg ggatgatagt aaagcgcaaa atgagttagg ggcaagcagc
     2221 gcttcttcag gtactgctaa tttcgttcaa ttcagagtac agagtcagat atgaatatta
     2281 actacagact acaacaagga ttttcattag tcgaattgat gatagccacc ttggttggac
     2341 tgatactcac ctattctatt ttacaaatat acctggcgca aacccaactc tataaagcct
     2401 ctaattctca ggatctgatt caaagcactg aaaatgccat agccaattta ctcataccaa
     2461 cgatcagatc ggctggattt gccggctgcg gatccatcat caccgcactt tccagtctaa
     2521 atgctggtgg cccaaggcct ttgggagtat taaatacaac accaacaatc ctgatgggtt
     2581 acaatggcag cggagcgagc tttaccataa cccaaagtaa tcctgcaaac agcaccaatg
     2641 ccagtcattg gtcacccgca ttagactcta cccttgttgg taatgctcaa caaggtagtg
     2701 atgtgttggt ggtattaggt tctacctctg acagctatcc aacaggaata actacgattg
     2761 atccaggcag caataccttt gtagttcaag gcactagcgg aatgtccctt acctctggaa
     2821 tgtttggatc ggtctctgat tgtggtaaaa ccgtaatatt tcaaatcact ggagtagcag
     2881 gaactacgat tactcatgat gcgggtgccg gagtactgca aaatgcaagc agtgctttcc
     2941 ctgtcaattt tcaaaccggc tcccaattca tacccatcca acaaactgca ttttttgtgg
     3001 gtcaaggtca aggagggcaa agtgctttaa tgcgggcctc tctcgttggc aacaactgga
     3061 caatacaacc attggttcct ggcgtggaaa tcatgaaagt acagtatggc attggcaata
     3121 atggtgtcat tactcgatac gtttctgcca atgccgtaac cgattgggca caggtctatg
     3181 ctattcgctt gggatttttg atagcaggtc aactcggatc aggaagctta acaacgagac
     3241 aatataacgt tttggatacg caaatcacgg tacccgctga taaccgctta aggcatgttt
     3301 ttgaattgac catcaacttg aggaactcga tatcatgaac ataaccccca caaatcaata
     3361 ttcaaaacaa cggggttatg tcctgataac ctctcttgtg cttatgtcaa tgcttaccgt
     3421 tttggccctg acccagatat cacaaaacac atctcaaacc aggattgcaa ctaacgcaac
     3481 cgattcggaa atcagttttg aaaaaactga aggcgccatt aatgaagcca ttaatctgat
     3541 cataaatggc acttataacc ctcaaagttt tcttgcttct aataatggaa tgtatttatt
     3601 tgaccaaagt gctactaccc cactttggaa aacgattaac tggtcaagtg gttcggcagt
     3661 cattaacagc tttcaaggca atacaggagc acaagccagc tatttccttg aaaaactgcc
     3721 ttccgtgatt atgccaggcc agagtatgaa aaagccaagt aatgtctata gaataactgc
     3781 ccggtcagca ggagcaagcg gggatacttc cgtactcata caaagtacaa ttcaaattca
     3841 acagtaggtt ttgattatca tggacgcttt aaaattttgg aatttaacca gaaagggaag
     3901 tcagaaagca ataaaagttc tacctcgaac actgatggct attctattgt ctgcttccgt
     3961 ttctctttat gccgttcctt tggcaattcc gcaaatccca ctcatcattg ccagtccggt
     4021 tcatcctcaa gtgctgattc tcctgagtaa ctcccaatcg atggatggta atttgagtgg
     4081 agcaatcatg acgggctccg gctctctgtc aactggttta agctcccttt acaattccag
     4141 ctcaccagtg aattataccg taccggctgg atttactccg ccagtacaag gccccgatgc
     4201 caacggcttg gctccttata ctgtagttca gggcggaaat aaagtagata acagcgcaag
     4261 ccgtctcaat gtggccaaag ccggaatcaa agcgattatt gaaaattaca tgcctaccac
     4321 cgactttgca ttaggcactt acagcacaag taacatcagt tcttataaca cttgggttta
     4381 ttacatgtcg ccacccggca gtgatttcgt gtttaccaac acccccgtag ctggaaatcg
     4441 atacgtcacc aacccttgtt ataattatgg ttccgcgtcg tctacggttt ccagcaactg
     4501 ctcatccatt ggaaacttgt atggcaccac tttggtttct tcaagtcagt atctgcaaat
     4561 tggcgattcc agcgatgatc cggatattaa cgacgcactc tacgctggaa gcgggtttcc
     4621 cggggttttt gtcagttata atggaccgac accatccagt ccttttcctc caaattatac
     4681 gatatccaat tataaccagg gcaacatcag aatttcctac gcgaacacaa gtccctctat
     4741 tggcaatttt tcctcctccc ccacgaacgc gggtttcgta cctttttcac agcaggtaat
     4801 gtatgtgcaa cggggttttg gttattacag caaccaatcc tatgccactg gtaatatgct
     4861 ggtgaacatg caaactgcgg gaaccaatcc aacgactacc agtgttaaca atgccattaa
     4921 tgccttttta ccccatttga agcctgaaac caactccaca gcgaccactg aaataaaagc
     4981 agctgcagta caatcccccc tcgccggact actgacccgc tctcgaagtt tcatgaaaac
     5041 tgtagggaca accagcggca attgtccgca aaaacaatac atcatattaa tttctgatgg
     5101 attgccaacc caggacctcc agtcaaggta ttggccaccc ctaggcagtg cggccgcgac
     5161 cggttatggg gtcaccgcaa catttaacgc cgatggctca ctgaacagca cgaacagtca
     5221 ggctctgtcc gatgcaataa acgaaataaa agctttaaaa aatgacggtg ttttaacttt
     5281 tataataggg atgggagcag gcgtggatcc tgctgtaaat cctgaggcag cagcgaccct
     5341 cagggccatg gccgttgcag gaggcactga aaactattat ccggcaacaa gtcctgaagc
     5401 tctggtcagc agtttaaatt ccattctatc gaatatccaa aatggctcct tttcaacctc
     5461 tgcagcagct atcagctcaa ctcgtataga cagtaatacc gtggagtatc aagccaactt
     5521 tattacaaga gacacacctt atcaggattg gactggtaat ttgacagcta ttgaactgga
     5581 tgagaatact ggccaaccaa caaatattat acaatggtca gctcaatcga cacttgattc
     5641 cttggtatca ggaaccggat ggtcgactaa ccgaaagata gccacttggg atcctatagc
     5701 agcagatgga ataccgtttc gctgggcaaa catcaatgca acccaacaag cacaactaca
     5761 accaagtgat ttgttaggag aaaaacgatt agaatacctg cgtggcaatg ccgccctgga
     5821 aaaaagaaac ggaggaacat tccgtgatcg ctcgcatgct ttgggagata ttatcgatag
     5881 ccaggtaatt tatgttgggc ccccaagtgc cccatattta accgccagtt acatcagctt
     5941 tgcaaaagcc aatgccaatc gacagccgat gttatacgtt ggggcaaatg acggaatgct
     6001 tcacgccttt aatgcaacca ctggagaaga gttttttgca tttattccca atgccgtgtt
     6061 taataatctc caccaactca cttctaccct gtacaaccaa aatcatttat tctttgtcaa
     6121 cggctctccg gaaagcgctg atgtacaatt ttccgatggt tcctggcata ccattctggt
     6181 gggaggtgaa aatgcaggtg gtaaaagcat ctttgcccta gacattacta atcccacaaa
     6241 cctgagttct gaaccaggag tggccaatgc tgtgctctgg gaatttacag attctgatct
     6301 agggctaact tatagcaaac ctcaaatagg tcaaatcaga tccggaagtc catcttcatt
     6361 aagttttgcc gtcttttttg gcaatggata caacaatact aacaacaaat cggtatttta
     6421 tgccgtcgac cctcaaacag gggcggtcct aagaaaaata gatctttgtg ctgctgtccc
     6481 ttcagcctgc aatgtcaatt taccccaggg attatcgaca gtcgctttgg ggcaaaaaga
     6541 cggattgcaa gctgatcctg ttactgtagt ttacgcaggt gatttacaag gcaatctgtg
     6601 ggcaatagat gtttcctcag ctgatcctgc cagttggagt gcgcgtgtat tattccaggc
     6661 gagagactct gctggtacac cgcagcctat aaccacacca cctttagtga cattaaaccc
     6721 taattatcct cgcaatcaag gtctttttgt tgtgtttgga actggacaac ttctgaccac
     6781 atccgatctg gtcactaccc aaagacaaac catttatgga gtatgggaca agccgctttc
     6841 atcagtacct tatgttcgtg ccaatctcca gcaacaaacg ttgaccttgg ttaactcagc
     6901 tacgtcaggt ttgtccacaa acattatcac tgccactgcc aataccatca attggagtaa
     6961 caaagtggga tggtttgccg atttgcctat agcgggacaa agactcatta ctacacctga
     7021 actaattaac ggggcattta ttgcaacaat aaatacccca cctcttaatt cgtgcggctt
     7081 tggattcaca tccatgcttc tggagcttaa tttcttaaat ggcggggctt tccaatatgc
     7141 gcgatttgat atttccggag atggcggctt tgatatagcc gatcagtata atggggccta
     7201 cccagttgga attggcttgt ccaacagtta tgctaactca ccaactgttc tggggcctaa
     7261 caaggataac aatctggtca ttctgatcac acaatcggat ggaacacaaa ccactgttat
     7321 tgcacctaac attacgccaa gaaaaatagg ctggtggcaa attcaataga ggatacccaa
     7381 atgctcagtc atgtacattt tatgaagaat agccgtatga aacaatccgc gtttaccctg
     7441 gttgaagttc tgatcagcat ggtcattatg ggaattctgg tttcaattgc ctatccatcc
     7501 tatttacaat atatacaaaa atcccgtcgt gctgatgctc atgccacatt gacacaagat
     7561 caaatcattt tagaacgctg ttattcacag aatttttcct atgcggcagc gtgtggcgcc
     7621 ctaccagcat ttcctcaaac aacgccgaat gggtattata ctatcaatat ttcaaacctg
     7681 acagccacaa cgtatacctt aaccgcaacc cctgtcggaa cccaagccaa agataccgag
     7741 tgtgccagta tgtcaattaa tcaagccaat gtaaaaacag ccgtagattc ctccgctaat
     7801 gcgcaaccag aatgctggaa tcccggttaa tactgtaaaa aattctttac acaaaggatt
     7861 caattcgagt agatttgcca gtaagggaaa gtccaactta atttgaaatg gaaatttttt
     7921 gctttttaat aggcgtactt tatgcctata cctataatat ttatctaact attgcgattc
     7981 ctttactctt ttatatcaca ccaagatatc caattgtcct gttcttcatc gccgggtgcg
     8041 gctttgccct gattcatcag tggattgtat ctcccaaggg catgcctgat acgcaagtaa
     8101 tcactcaagc ccagttggaa ggaacaattg cttccatccc catgcaacgt cctaacaaaa
     8161 ctcaattcca ttttgcaatt gaaaaaatta ataataaccc tgctcaaggc ttgatccaat
     8221 taaactggta taacaaacct cctgtcttac gagccggtca gcgatggcaa ctttcagtga
     8281 aactaaaaaa acctcgtaat ttccgcaacc caggaggttt taattatgta cgttacctgg
     8341 cggctcgtca tatccattgg actggttata ttcgttatgg gaataataag ctaattagca
     8401 atcaaccccc ttcttttagt tggttaacac tgcgtgaaaa aataggggat gctttgacaa
     8461 aactggcacc gaatacagaa acggcaggca tcatcgaagc attgacgtta aatgttactc
     8521 accatatcag ttcagaacat tggaatttgt tccgaagaac aggaacggta catttattcg
     8581 ggatttcagg ggagcacatc gctctggtct ccggcatttt ctttatagtg ataagatgga
     8641 tttggtcaat gagctatcgt tgctgcttgt tcttaccggc ccataccata gcgagtatcg
     8701 gtgggctact ggccgccttg ccctatgctt ttttagccgg gtttgaacct cctgtacagc
     8761 gtgctttaat tggttgtgta ctttacacaa tatacgccat aggaaaacaa cgttttacac
     8821 catggcaaat atggagatac gccttatttt tagtcttgtg tattgaaccc catgccgtat
     8881 tcctgcaagg tttttatttt tcatttcttg ctgtggcctg tcttctattg acgcaccaac
     8941 gttggccact gaaaaattat aaaaaaacac tgacattaca attaagctgc ctcattggat
     9001 tgatgccctt gactctccat tggtactcct atggttctat taatggattc cttgccaatt
     9061 tattctccat acctttagtc agctttctga tagtaccctt ggctttaatg actatgataa
     9121 cgagtccgct tcattggtcc tggatattga tgaaaccatt ggcatggctt gttgccctgt
     9181 tactgcaagg actaacgtat gttgagcaaa tagcagggat taatatcacc tggtccatca
     9241 cctcggtaac aatgatgctc gctatgacag caggtctgtt tttaagggta ttattacctg
     9301 tcaagccatt caaacacttg gctatccttt ggattatcct tcccttattt ccgcctcgta
     9361 ctgcaattta tccaggggaa gcattaatta atattttgga tgttgggcaa ggactggctg
     9421 ttgcgataca aacgcaacat catgttttac tctacgatac gggagattcc tattttcagg
     9481 ggagtgattt agggcaaatg gcgataatac cctttttcag gtcaattgga ataaaaacaa
     9541 ttgacaaaat agtaataagc catcctgata aagatcataa gggcggatta aattcccttg
     9601 aaaaagagat gcaagtcaat caattaatag tgaatgatcc ctatttttat aaacgtggaa
     9661 gtaattgtca ttcataccca caatggcaat gggatggggt gagttttcgt tttttaccca
     9721 tcaaggaatc tttcactaaa aaaaataaca attcttgtat cttacaggta agtaattcag
     9781 ctggcagggt tttattaacg ggagatattg aaaaagcggc tgaagagtac ctggtaagaa
     9841 actacggcag tcagctggca tccgagattt tagttgtgcc tcatcatggg agtaaaacgt
     9901 cttcatccta tcgatttttg ctggaagtaa acccgcttta tgccattgca tccttaggat
     9961 ttgataatcg tttcaaattt ccacattcta aaactttgca aagcatgaaa acattagaca
    10021 tcactttttt tagaacagac gaatgcggaa tggcagcaat caaactacct gcatcaggtc
    10081 aagttcacag accaagttgt atgcaggcta attgaataga tgcattggct ggccaatgta
    10141 ttttacttca tcattccaag tgctatacct ctaagtctta ttagccgatt ggaaccacca
    10201 gtcactcaat ggtgtattat tgtaatactt taaatgttca gtaaaaaatt gtaacgcttt
    10261 gtccttttga ttagtgtgta tcaacaccaa caagtaagtc tggaaaaaaa gctctatctg
    10321 tgcatcgctt ccacctaatt gtgcacactc accaatcacc ggctccatca acatcaaggc
    10381 agttttgtaa tctgcatcag caaaggcata gataccctgg cacaaaggta aaacaacatc
    10441 gccccataga gcatcagaag aaggcgcaca ggcgtaagac ttcattcttt tcaacgactc
    10501 atccgcttcg tttttataac ccgatttcac cagacaataa ataaaatgag cgttcgtaaa
    10561 cccggtataa aattccaaag gatgggtact taaatggtcc acaacctgat ttaacagcct
    10621 gtcttggggt agacctgcca tatccatgcg ccataacagg gaaatagtat ccaactgctc
    10681 aagcaccgta tcaggcaatg cccctgaaat atgggggtat aactttttaa cctcctcttc
    10741 atcacgattt gccaaataaa atagcgctaa atgccaggtg ttatggcctt tcaacaaagg
    10801 taaaatgtcc tcccaggttt tttgtaaatc tcgaagtcta ttgataccac cagtgatatc
    10861 acttgttaat aaatgcacat gagccagggt atgatgtgcc caaggggtat gcaaattcat
    10921 tgtgatagct tcctcagcca tttccctggc ttttgaatat tgcccacaca actccaaagc
    10981 aaaagaatgt atggctaaaa aatgggactc atcctgattc tcgggagcgc acttatcaca
    11041 tactttaaga aaatactcag cattaaaagc ctgtcctgta cagtaaaaaa gccattccac
    11101 gaatttaaga gccagggtat ccctgggata cctttcaagc agagtaacta acaagctaat
    11161 ggcattggca taatctcgct tagaccaatt gtttactgct tcaaaagtta atttttcacg
    11221 ttcattcgct gatgttaata acctggatgc tgattgcaaa taatcgctgg cttgttcatt
    11281 cgctacatct tcttgtgcat acaaataaaa agcagccgca taagtttgaa ttaataaatt
    11341 gtcagtatgt ttttttgctg aatccagaat ttggattgcg ttttggccgg agcttaatac
    11401 ctgttcgtga aaataattga tactatctac tgcctctatg gagcctgtcg ttacttccaa
    11461 tccacgttga gtatgcattc tcgaaatcct gtcaatgaat gaatcaatga ttaaatgata
    11521 gcacaaataa taaacaaaaa ggacggaagt aacgctccaa ttagataata atacgacctg
    11581 atatcacgat gacagcggta gagctacaag cactcttgct aaaattgttt ttcctggcca
    11641 ggaattaata caacatcaga ttacccactt cccgctaatc atgctttatc attgagttga
    11701 attaccatca cctgaattga atggttagtt acgttttcat cgttatgtaa ttcgttagga
    11761 acgtctttgg taagataata cgacttgcct ttctttaatt ttaaatagtg tactttccct
    11821 ttgttgtttg taattttcaa tgtcccatcc gtcaatgcga ccaaaactcg atcgttatca
    11881 tgcctgtgca tgggcaaaac ttgttttgaa gagggataga taattgtttt ccacacggtt
    11941 accctggcat tagataattg ttcaatacgt tgggttgata atcctgcagc ctgaagtgca
    12001 cttgtaccca gaacaaagat cataagcaat ttgaataaat taattttttt catcatgtta
    12061 gattactctc cattttcaaa tcaaataaca gtgtgtcatt tcgcttattc ctcaattttc
    12121 ctttaacttt ccggcacata gctcacaaaa ttgctataat tatagtagga ggtaattgta
    12181 aggagtttta agatgttaac tacatttcta gccacagtga ttggctggta tttagtcata
    12241 gtaagcttat tcttattatt ccataatgag catgcaaaat cagctatggc agagattata
    12301 gctcatcgcg gatcgcaatt gattatggct atagtaacct tgattacagg tttgctttta
    12361 gtcgtcagtc ataatatctg ggtaatgggc tggcctgttg ttattactct ttttgcatgg
    12421 ttagtcttgc ttggtggttt aataagattt ttatgccccg ataccgcagt tagagtaggc
    12481 caatcatttg tcgataatcc aatgagacta aaaatagcag ctgtaatatt ctttattatt
    12541 ggtttgttcc tgttatttaa tgtctattat gtttatttga aataaaggaa gagttattgg
    12601 ggtactgtga ctcattctgt ttatggggaa aaggaagcga taaagtcctt gtcaattcag
    12661 cagcaaggaa tgctatgata tgatgcttga tatgatatac ctacaaaaaa atgactagag
    12721 cccaaaccca ccctcatcaa acgaccgtta tttcaaacct caaaataatt ccactcactt
    12781 tggggtggtt attaaaattt gctttgctca ttattataat cgctgttttt actccactat
    12841 cccccaaaat gccagctccc ggtcttgatg cttcttgggc tgtaggactt aatcaagccg
    12901 ttgcccaagg tttggccttt ggtaaagaca ttttttttac cttagggcct tattcttcca
    12961 tctatacaaa attctaccac cctgctacca attttatgat ggttggggga agtttgtatt
    13021 tggctttatg ttactggata gctattattc ttttaatgca aggcattaaa tggcgttgga
    13081 gtattgcttt tgcaatactt ctctttagta tgatttatgc tcgggattct ttgttttttt
    13141 cctattcatt aatcattggc ctgctcattc ataaagaaat tcctggaaaa gaaacatcct
    13201 tgaaaagcaa tttcttcact ttatttgctt tgctttttac gccattcggt ttatttcccc
    13261 tgatcaaggg ctctctttta atattaagta ttattaccat actcttatgc tctttctttt
    13321 tgagcctata taaacagcgt aatcttgttg tgctttgtct ggcatctcca gtagtttcca
    13381 tattgttttt ttggatagca tccgggcaat ctgttttcaa tctgccagct tatatcagta
    13441 acactatctc gctcgcatca ggttttactg aagccatggc aactgaagga gacaataaag
    13501 aactaatatt ttttgggatt acctcttctt gcattctctt atcaatactc ctgcaaaaac
    13561 agctcttacc attatccaaa gcatttctat tttgcatcta ttttgttttt cttttcctct
    13621 cttataaaac ggggttcaca agacattttg gccatgcctt cattgctggt acctctgttt
    13681 tgctagcctc ttttttattg ccgtatttta attccaacaa caaagtgata gtcccattat
    13741 tagctctatc cctttataca gcgaattata tcaacgggca ctataccaaa atatctgttc
    13801 gagataattt catttccacc tataccgccg cttactacgg ccttcaaaac agaatccaga
    13861 ataagaattg gttaaaagaa aatttttcat taacgatgaa ttatttgaaa gcccgtgatg
    13921 atttccccag attaccaggt actaccgata tttattccta caatcaaact tcattaatcg
    13981 cctcgggaat gcaatggtcg ccaaggccaa tctttcagag ttattccgtt tttactgcaa
    14041 aaatggcaga aatcaatacg aattaccttt taagcgagaa caaaccggat aacatcattt
    14101 ttaaagtaga accaattgac aatagaattc cttcaatgga agacgggatg agttggccga
    14161 cactgattaa tcattatcaa ccagtacggt tagaaaatga ttttttattt ttaagaaaaa
    14221 aattagttaa caaacctgtt acatctttag tgccattaaa aacagaaatt catacttttg
    14281 gagaacaaat taaaatacct gataccgata aacctctttt tgtcgaaata gaaattcaac
    14341 caacgctctg ggggaggtta gcgactacat tttttaagcc tcaccagctt gaagccaatt
    14401 tggagttaaa aaatggtacc caaagacaat acaggctcat ttccaatatg gcaaaatccg
    14461 gttttctatt atcgccttta atagaaaaca gcgctgaatt tggattgtta tttagccaga
    14521 ataattacct ggcaggtaaa aaagttattt ctttttccat taactcaaag caagtgcctt
    14581 cctcgcactg gcaaacgaaa tatactattc attataaaat ttttgccagc aataattaaa
    14641 acactatgga tgaggcaatc aaaatagtat accaagctct tctttgttag tttttgttta
    14701 aattccaaac aaatatcttc ccaaaaggaa aattaacaag taaaattatg tactaaaata
    14761 attcatatag tttactaagt gtctttaggg ttctttttat ggccaaaaaa tatcgaacgc
    14821 ccattccatt gtcagacaaa atgatgactc ttaaaaagaa tcttcaggat caactgacac
    14881 aatcccaatc gttaggtgag caaattcatg ctggaactac ggcagcatca acaataacaa
    14941 cgggagttag tcatgtgttt aatgtcattg actccttttt aagcgcctat cgacaaattc
    15001 cactggtagg cggaatactg caactagtgg ccacagttgc caaatcaatc tcaattgtga
    15061 gcgacccgga aaaatctatt gctgaaaaaa taatatcgtc gattctaatt gctgctatta
    15121 tcgccctttc tattacagcg atcacgctag gtgggctggc tgccgcaatc atagggacag
    15181 tgattgcctc tactgtaacc atcatggaag gattaggatt ttttgggaaa attgctgaaa
    15241 aatttcaatt ggcaaattcc tacaaaatga aaaaagaatt tatagatctt gtagcgaaac
    15301 gtatagagcc agacaatgat aaatataatg acttattaga aattcgtgcc atagaactcc
    15361 aacatgaact tgagaaatct tatcccaatc aaattgaaca gcaacaatta aaagaagagt
    15421 tgaatttcat taatacggta cttaggaaaa aaaatatcac cccgggaaac aacaaagaga
    15481 atgacgctta caaactaaac caactctatg ctaaacgtgc tgagcaatta aaaacccttg
    15541 tagataaaat tgctttaatc aatccaactg gaaagagagc tgagcaaaac aaacttttaa
    15601 gcgaaatcaa atcattacaa aaagaaataa ccaaaaccga cgatagcata gaagaaatca
    15661 tagccccgct tgctaaaata gaacgagaca atttgaaatc taccattaat ttgccattaa
    15721 gttacagcac ctttgctata gcagcggcca gtactttaat atcgatagcc ggcttattgg
    15781 tggtggctgg cgtgattgcc gcacctccga ttatagtccc tatcatgttt gcagtaggta
    15841 gtgttatggc aatcattggt ttaatcaagt ggaccgctga aaaaatggct gaaatagagg
    15901 atgctcaatt aaaagcagaa gaaactgcga aacacgagga tgcagttttg gatgaagcat
    15961 taaacgttta tgagcaacaa ttaaatcttg agttagtcag ccctgcaaac cctgcgagac
    16021 aggtgaaaaa tatattaaca gttcaagata aagtgcctgc tttgtcgcca actgaaagta
    16081 ctgtaagctc attagattcc agatttactt tatttggctc atcttctcga gagcacacaa
    16141 aaatagcaga acagaaaacc aatgatacaa ctaatgaaca agcacctaca acttttgatt
    16201 ccatatccaa accaactcgt taacaaatat aacaactttt tataattttg ttataattga
    16261 tttataaata gttagtttat gcataatacc tgattcgtga atataaaaat taatgaaatc
    16321 aagagaaaaa ttcatggcca gaaataaaag actcgatcgt tttcataatg ctcaaaatag
    16381 taatacttgt ggctacgatc aagcattcag cgaaatacaa ggcggaagaa aaagaagcca
    16441 ttggatatgg tatatttttc ctcaattgga aaatttaggt aaaagcccat cggccaaact
    16501 atatggaatt aaagatttta atgaggcatg tgattattta aaagatcctg tactgtttaa
    16561 gcgctatttt gagttaataa aactcataga ggaaaaatta aagaacattc ctctacctca
    16621 tctgatgggt ggttttcctg atgatcgtaa attcatctca agtttgacct tgttccaagc
    16681 cacttccaat tacctgagtt caaaagatac ggattctcaa aacaagtatc atgagcttta
    16741 tgagacttgt aatagagttt ttaaacaaat tgctccacag ggttattctc catgtcaaca
    16801 aactgagtta ttggtcaaac atcaatcaaa ttcccctgtg aaaaagacaa cagaaaaatc
    16861 aagtcaacct ccccaagtga ctatcttttc acccaaacgg tccaaatcga atactgctca
    16921 ccctatcctt aaggatcttg ctcaatatat aaacaagagg ggaaatgaat ggagttatca
    16981 ttacaatttt ctaggcctag tggctttcgt ttatttcata caggatttaa ttcttggaac
    17041 cgattatttg aatgataaag ctcgtcaagt caaattaact gcggcaagca aattacagaa
    17101 aattcttgat ccccaatgcc atgaacgatg taatctaaat ccatcagaaa gaaaggcgct
    17161 ttccgatggt cgcttggggg agattattaa taaacacggg ggattagata aaatcctgac
    17221 agaggctcct aggaaacctc caaaaactgg ccacgatcag gaaatcaata ttagtccctt
    17281 ttcataaaac cattggattt caagtctttg caccaagaaa catcacatta taaacccaat
    17341 ggcttttaca attttttaat ctctacgaat tcactacaaa ccacatcaat tattattcaa
    17401 aagactccca tagagcgaca tatgcaccag tcggatcgac tatcattact tgacaacgaa
    17461 tacaacttaa tgaatagttc acacagaata gtagaccaat gttataaata ctgccattac
    17521 caggattaaa tatttttaac ccaatctaat aattacatta tgagtcaaaa catcattcta
    17581 aaccgatgcg aatgggtagg gcaaaacaaa ccccattatg aactatacca tgatactgaa
    17641 tggggaattc ctgttcatga tgatgaaaaa catttcgaga tgctcattct ggaaggtgct
    17701 caggcagggt taaattggga aactatatta aaaaagcgcg atggataccg gagagctttc
    17761 aagcaattta acccacaagc cgtagcccaa atgactgatg atgaattgaa tgctttgtta
    17821 attaatcctg aaattatccg caaccggctt aaaatctttt ctaccagaaa aaatgcgaag
    17881 gtttttctat ctattcaaca agaatatgga tcgtttgatt cttacgtttg gcaatttgtt
    17941 aatggggcac ctatcttaaa tcgccctgaa agcatacgag cgataccagc aaccagcaaa
    18001 gaatcagatg ccttatccaa agatctgaaa aaaagaggaa tgagctttgt tggctcaaca
    18061 atcatctacg cttacatgca agccattggt atggtgaatg atcacatcgt aacctgtttt
    18121 cgagccaaat aaagaaatca ctcgaactcc tgaaggtgac tcatattctc cataagcttt
    18181 ttagcgaaat catttaaacc ggattatctg tcgtgtcctg gcctaaccat aaattagaaa
    18241 ttcggttttc atattttgtt aggccaggga atgatagttt gaccaaccta atcaggttct
    18301 caagctgact gtaatgtaag ggccacctac agaaaaatta ctttgtgtgt gtttggcact
    18361 tgatgtcgac aaagagccag tgtccaaggc aataacattg gtatttgttt cataactgga
    18421 taaaggatta aaatacaaag ctcccatata ccctgcttca aaattcaaat cccaattttg
    18481 tttcaatgag taaagataac ttaacccaat tttggcatcc agagctggaa ccagttgatt
    18541 gacactggga ttagcaagtc cttcacgatt tacaaagatt ccagcgataa tcaattgctg
    18601 agaggttcct gtgaattggt attgagctgg ttgcattctg ccgtacaaga ctgagccggc
    18661 gagttgtccg accaaattga acccatgaga aatattataa ctattattga atcccagtct
    18721 cggcccaaca cctttgtaag tggatgtatt atttaatgac agtataggga gtgaaaaacc
    18781 ccgaaaagtt gatatcaact cttcctttat ccaggcactg cttaaccctg taaaaagcct
    18841 tgtctgtaat ttggaaccaa agttaataaa ttttccaaca tcgagactga ctgcatcata
    18901 actaaaatca agactggcat gtgcaagctt caataaagcc actcctgtaa tttggccaga
    18961 aggtgtagga ggagtaccgg tctgagaaaa aggagagatc cattgcgatg agggattcac
    19021 aaagacatta tcagtatcat tggtacgcag atgagaccag tttaattgca aatcgcttcc
    19081 agaattggca aatacataac gtcctcccaa attaaaccca acttgataat ctggattaaa
    19141 agtaacaacc tgccaattag gtgatggtat tggcagtact gtcgtaatca ccccccaacc
    19201 taaattatcc gctcccggtt gcagataaaa agctgtcgca gaaaattcat atccaggttt
    19261 caaattagga atgaagactc ggaacaggtc gctattttca actggccctg tatcaccggc
    19321 ataattacat acaccaaaca acagtaaagt aattcccata agtaattgat gatatttctt
    19381 catatttaat gaatcctccc taatgcatga tttctatcaa tcaggactta cgaaaaaacc
    19441 caatctctat gttaaaaaat gttttaattc ggtcatttag tttatctaaa ctccctcatt
    19501 aaaaacattt tttgccttga ctttgggctt ttcgtaagtc ctgtcaatca aaataaactc
    19561 caaaaccaag tttttattct ttaatctatg gatttcagcg atggaccaat cgaacagcga
    19621 tgagtaaatc tgtggaatta agtttaagat ttatgcctga attggtacaa atttccaggc
    19681 aatcttagca taggaaagat gtaatgtatt gaaaattaat tattaaattc ccaggcggga
    19741 ttaaaattta tcccttaggg gataagtacc ttcttttatt ggcgtattaa acgaatattt
    19801 tgcccatcaa aagcacaagg ttctgttgac ctgattgggt taatatccag acctcccctt
    19861 cttgtatatc gtccataaac agtcaatgac tcaggttggc aaaatcgcat aatgtctgca
    19921 aatatccgtt caatgcactg ctcatgaaat tcattatgat ttctaaatga aatcaggtat
    19981 ttaagcaaac cttcatgatt tatcttttta cctttataaa caatttgaac gcttccccaa
    20041 tccggttgat tggtaaccag gcagtttgat ttgagtaaat cagaatacaa tacctcttca
    20101 actaattcag acgaggtcga aagaaaatca ggcattacat gatacactga gcattcaata
    20161 tcaagcttat caaggcaaac accatcgaat gcagcataga tagtttgatt agggatatcc
    20221 gacaaggggt atacattaac cgccacttca gagtcaatgc agtgacttaa atctttgctg
    20281 atagtttgaa caacggtttc cacgtcaggg aaattagtat tattcaacga gttaaaatag
    20341 agttttaatg acttggattc aataatgcac ggagaatgac aatcataaga aataacagcc
    20401 atcgcaacaa caggtttgcc tttggagttt aaccaggaca cctcataatg attccagcaa
    20461 tcaaatccat agaaagtggt ggagttggga tccaaattaa tttcctggcg ttttggggct
    20521 ctggggatag gatacagcct tttaggatta tagtagctgt cataatttgc agtttgtccc
    20581 agctccgatt gatttgctat attctgataa ataatttcaa gttccttatt cattatggat
    20641 gccttttgta actctaaaaa acaaagtgat tataatctat catgttacct aagggtcaat
    20701 tttttcttaa aaaaaaagga aatagcctat atttaattaa agatactgaa attagcatct
    20761 aaaggagtgc atttacccat gattaatcaa cagatagaag tatttttgaa atctcctgct
    20821 tttgccgtag ttggcgcctc ttccaatagg gaaaaatacg gcaacaaagt attgcgatgc
    20881 tatcttcgga atggcaaaaa agtctatcct gtaaatccac atgaaacgct gattgaaagc
    20941 ttgcctgtta ttcattcagt aaacgattta cctgatgatg tacacagtat ttctattatc
    21001 acccctccat ccattactga agaagtcgtc aaggaagcag tcaaaaaagg aatccaacat
    21061 atatggatgc agcctggagc agagagtgac tcggccattc aatatggctt ggatcacaag
    21121 cttaatgtca tttctggggg accttgtata ttggttgtca tggggtatga agagaattaa
    21181 gaaaatacaa aaagctattt tgtagtccaa aatagctttt ttgattttgt ataatcccag
    21241 ccgattattt agaacgcctg aattgcttta atgattcatt gaccacactt tcaagagttc
    21301 tatgttctgc atatttttct cttaaaaagc tggcatcact ccctttatga gttatttcat
    21361 aaatatgctc caaagccata gtactattta gctgattagc ataaggcttt aatcggcgaa
    21421 gtgttgttaa aatatcttct cgaagcaaaa tttgctcata tgttttggga tgcaccagag
    21481 tgccatccag accaaatcgg catgcttgaa aacgattgta attgtacaca agataatcat
    21541 cttcgtgagg cagaggttct ttattttcca ataaataact gcaaagcgct tgcatataac
    21601 atgctaaagc ggctgctcgc tcaaccgtaa ggggactatc acacacccgc atttcaattg
    21661 tcccaaattc aggtttaggt cttaaatccc aataaaaatc cttcatactt ttcactatgc
    21721 cggtatgctc cattttttcg aaataaccga gtgagaattc atcccagttt aatacaaagg
    21781 gagcccttcc acttaatggg aaggcaaata ccgaattcaa tcgagcagag ttatacaagg
    21841 tatccttact ctgaacgaag ggtgatgaag cagacaatgc aataaaatga gggatataac
    21901 gatttaaaga atgaagtaaa aaaagcgctt gattaccatc ctcacaacca atatgtatat
    21961 gttgcccaaa aatagtaaat tgcttggtta gatagccata taattcagac acctctataa
    22021 acctggtttt attaaaaatt cgctgatcag accacatttg aaatggatgg gttcccccgc
    22081 cacaaatacc aatatttaat cggtctccaa ctgtcaccag ggtatcacgt atatgaaaca
    22141 gttgtttcag tagttgatca tattcttcat gcacatctgt cgctatttct aacatacttt
    22201 ctgtgatttc tggcgtaaaa ctgccgggaa aagacgatcg tcccaagagc tccaatatat
    22261 cagggctagc ggcagtcaag tcaaaattac ttaagctgat taattgtaat tccagctcaa
    22321 cccccatagt cagtaaatta gatgttttga atggttgtaa aggcatttca aaattccttg
    22381 attgcagaga tctcgttcgc tttaattaaa gcacgttgaa ccattattgg acccaatatt
    22441 tccaaagtca gagcaaccat agccagcggg gccagtgtat ctaccaaatt aataccaata
    22501 tatcgactct gttctaaaat caaaatgaca aaaacagaga tgggggtcat agccaaacca
    22561 gtcaagatac cttttttaaa cgaaatccca ctaatatgag caaataaact aatcccgata
    22621 attttagcta cgaaccttac agttataatt gataatccta tccatacacc agttactacc
    22681 ctttgccatt ctatggttgc tgcaataaat acaaacaaaa gtaccgataa caaatcacct
    22741 aaagtgccaa atcctggctg tgaaggagaa aagaccattc gcccatggcg ggaaaccaat
    22801 ccaaaggtta atgtggctaa aaccggggac aattttaaat tatgggttaa gataaccagc
    22861 agtatcacag cgcaggcaaa tgccaacgta ttgtcttgac aggtgctttt aactgtttta
    22921 agcaaaatgg gcattatcac tccaaaaata atcccccaaa tagttgaaat ggctgtcacc
    22981 gttaagctgg cataagctgc ttcccaaaga tttcctgaag ttctgaaaac aaccaagcca
    23041 atgatgactt tgaataaaaa tactgccagc acacaattga tcacggagag atgtaaaaca
    23101 cgttctgtca cttggcctga actacgttgt tcattaatca ccctgaccac agtggctggt
    23161 gaagtcgcca tggacaacga ggccaataaa aggcaggtcg aagtttgtaa cccaaaggat
    23221 ttcactaaaa tataaaccag gcaaaaggtt aaagtcgctt cggtaatact ggatgctgca
    23281 acccaggggt tataacttaa ccagcgtaaa ttaattctat agcctgattc aaacaagatt
    23341 agcccaaaac caatattggc taaaagtaaa atagatggtg gttgaataga cggtaataat
    23401 cctatttgag ttgatgctag tgaaaatcct acaatcgcat agatactgat atgaggcaat
    23461 tttgcccatc gatatccaag ttcccctgca aaccaagcta tcaataaact gataggccaa
    23521 gccaagtcgg ttataatttt tccaacatca atcatccata cctcctatga aaatccgacc
    23581 ttactttata acacacatcg atttgttttg cagaattgaa gtatttttaa gacaatataa
    23641 aataaccagg acttacgaaa aatcctaagg tcgaggcaga aaatgttttt aatgagggag
    23701 tttagataaa gtgaatgacc gaattaaaaa cttttttaac aaagagattg aggtttttcg
    23761 tgagtactga tagttttttg tccaattagg aaaatcattg ctactaacca cattaacgag
    23821 aaccaaaaag aaatctcaac aacttcttat tttaataggc ttcttattag ggatgtcacc
    23881 agtcatggct ttagaaatta ttacaataga aaaaaaagaa aactaccata tgctaaaaac
    23941 tcgggctgaa aatattcaat ttcccttgaa tcgagaagat aaagatttaa tagaagcgat
    24001 gaaaaaaaaa ttatacgcat tgggaggggt tggtttggca gcaccacaag tcaattccgc
    24061 caaacaaatt atcgcagttt acatccctga agaagctcag cttttacgtg ataatgcgaa
    24121 aatctttcca atgcacatcc taatcaatcc aaattacgag cccctgcccc acactcgtat
    24181 tattagcgac tttgaaggtt gttattctgt atccagtaaa gcagggaaag tgccaagata
    24241 tgatgaaatt cagctaaagt actatgatga agaagggaat gttcaccaac aaatcgaaaa
    24301 tggcttttac gcccgtgttt tgcaacatga aattgatcat ctgaatggcg tattaatcac
    24361 cgatcgttta actcccgatt gtgtacaagg gactattgaa gaaatgatga ctattcgccg
    24421 tgctgaattg cctgtagaaa agcgaaaatt atttgatcaa atcatggcaa aaaaacttaa
    24481 aaaataaggt cgagtttcca aattatcttt tatgaattga tttcaatgat aaactgccct
    24541 gggtaatttt tttgcttgtt caacccaggt aacaacttga gataatccag gtacatgttg
    24601 cgccaaatga cattgctcat ttttctcttt taatgagcta taaagttgtt caggattgca
    24661 atgtgctaac tcagggattg agtcaatacc agcctgctcc agaagctcag catattcttc
    24721 attaatgcca tttactcgtg caagatcagc ttgattggtc catttcaaaa tcaatttatg
    24781 actgattccc gtttgctcgg ctactctaaa acgcccccgg cgaccactac aaactcttaa
    24841 taaatcgtcc tgactttcaa tccctactcg ctttagcatt tcagcatatt ttgggcctac
    24901 gccttgcagc tcaattaact tgtgcatttt atttccagtc ttctactacg atattacata
    24961 tataaaatta tagaatataa tgattaatta ttaaccaaaa caaatgttga aaaaatttag
    25021 gcctcacgta ttaaaaaaaa atcaattcat ttggcaaaat gaaacaattt cattatgata
    25081 attactttcc tttcagtccc tggtgaggta gaaaatgcgc aagcacaaga acaaaatggt
    25141 caggatattt ttattggctt taggactatt ttccagcacc accattaatg cttggtatgg
    25201 ctcagggata accattggtg ttgccagagc gaatgttcac tattatggag atgatatccg
    25261 agtatatgac ccgtggggct atggatacta cgacagaaat tattactata atggagtaag
    25321 aaacattatc gttcctaatg taattgtgcc gaatgtagtg atcaatgtac cagttcaaag
    25381 gtattatgta ccctcatgtg aggaaatgga agtttgtgat ccttatggac aatgttggtt
    25441 agagcaattt tgcagataaa ttaaaaactc aatcaactaa tgttttggga ctcagcccat
    25501 tatgggatga gatatcagca ggacttacaa aaatcccaag atcaagacaa aaaatgcttt
    25561 taatgaggga gtttagataa actaaacaac cgaattaaaa acattttcta acaaagagat
    25621 tgggggtatg tcctgattat aattttttaa ggacaaaaaa tgtcagaaac tattggttat
    25681 gccgcccaca aagcgggagc ccctttaaaa cctttcactt ttgaacgccg ccctgttggt
    25741 gcacacgacg tagcaattga tattctattt tgtggtatct gtcattccga tttgcatcaa
    25801 gtcaataatg attgggcagg agcgttctat cctatggttc ctggacatga aatcattgga
    25861 cgagtcaagg aggtaggaca tcaagtccgc cggttcaaag caggagattt agtgggcgtt
    25921 ggctgtatgg ttgactcctg ccgtcaatgc acctattgcc atcagaatga agaacaatat
    25981 tgcaaatctg gagcaacttt tacctataat agtcaagaca atcatctggg aggggtgacc
    26041 tacggggggt attccaaaca tattgtcgtc actgaagatt ttgtattaaa aattgatgaa
    26101 tcacttgatc ctgccgccgc cgcaccatta ctctgtgccg gaataaccac ttattccccc
    26161 ttgaaaaggg ctaaagtagg taaaatgagt aaagtaggaa tcgtaggcct gggtggctta
    26221 ggacatatgg ctgtcaaatt agcccgtgcc atgggggctt atgtgactgt atttaccact
    26281 tctataaaca agataaaagc gggtaaagat ttgggggctc atgatgttgt cttgtccacc
    26341 gacagcaatg tcatgcaaac actagccaat caatatgatt ttattcttga tactgtttct
    26401 gccaaacatg atcttggcca atatttaaac cttcttaaag tggatggaca actaacccag
    26461 gttgggcttc catcagatcc acttgaagtt accatgtttc ctgttatttt aaaaagactg
    26521 agcattaacg gctcattgat tggcggcatt aaagagacac aagaagtttt ggatttttgc
    26581 gccaggcatg ggatcaccgc agacattgaa ttaataaaaa tgcatgaagt aaattctgcc
    26641 tatgaaagac tgttaaaagg agatgtgaga tatcgatttg ttatcgatat gtcaaccctg
    26701 gattaaatga accaggtata aaaggcttcc agttggcctt ttataccacg gagcaaatat
    26761 tattcctggt ttttgatctt ggggaaaact ttaattttgg ttataaaaga atcctgtacc
    26821 ttttcaatca aggcatcaaa ttcaacaaat tgaattattt cgccttcatt gggcaatttt
    26881 ccgtggcgcg tgataataag tccatatagt gtattcagtt cttcttcatc tggcgataaa
    26941 gtaatatcgc aatcaagagc ccgttctaga gaataaaggg agcaatctcc ttttgtcgtt
    27001 atagttccgt ccgaattttt tacccaatct tcatgtgttt tatgaaactc gtctttaatc
    27061 cgcccaatca ggatatggag caaattatcc agagttacaa atccaagtaa actcgatttg
    27121 tgtttgtata ccaaagcaaa atgcggcata ccagctctga atttacgcag caaatccata
    27181 gcaggtaatt tatgactgac tttaagtact ggtcgcacat aaggctttaa tgtattgata
    27241 tttttctgga gcaacaaatc ggcaaataaa tccttaacat ggatgacacc aatcatttcc
    27301 ttagtcccgg aatcatagac aggataacga ctataacgat attttaaaat tttatcttta
    27361 atcgtttcga atggctcatt gatatctatt atgatcattt catcttcagg gcgcattacc
    27421 tcagtgacat ttaactcagc caaatcaaga gtatgctcga ggatttcacg ttcatcctct
    27481 tttaattccg attgcagatg ctgcccgctt aaaattaatt taagttcttc acttgaataa
    27541 aaaccttcgt attcgttttt tatatccatg ccggacagtt tgagaaaaaa attggaacag
    27601 gtatttaata accagatggc aggatacatt atccagtaaa accaatacaa cgggactgca
    27661 gtccatattg aaatcaattc agattgccga atggcaatgg acttgggcat aagctcaccg
    27721 attacaatat gtagaaaaga aataagaaaa aatgcaataa aaaaagaaga tatagtaaca
    27781 atatgaaccg atgaaatacc caaagcaaca aataatggcc ctaataactc tgcaaaagca
    27841 ggctctccaa tccaacccaa tcctaacgaa gcaagagtaa tgcctaactg acatgctgat
    27901 aaatacgcat ccagattttg atggacttgc tcaagaatgc ggcctcttag cccgtaattt
    27961 tctttgatgg cttgaattct cgtagccctg agtttaacca tcccaaattc ggccgataca
    28021 aaaaatgcat tgagaagcac cagcaggaat gctataaaaa ttaacagaat gttatacatt
    28081 aaaaatagtc cggtttatga tttaatttca cagaacttac gaaaaatccc attctcttcg
    28141 ttaaaaatat ttataattcg gtcatttggc ctatataaaa tccctcatta aaaatatttt
    28201 ttaccttggc cctctggttt ttcgtaagtc ctgttttcta ttcagtcgta ccttcactaa
    28261 aaacattcac tctctacaaa tgtgatcgaa tcctttatgt taatagaata tcgcagtcct
    28321 ggcgagttta aaatgactaa gccagaaccc aaagatacaa taattatcat ccggtttaat
    28381 gacaaattat ttaatcaaag caaccgtata gaaatgacac caaataccat taaatggcgg
    28441 ccccctattg acaaatagaa gcacaatttg ttaatttgac tcattaaaag acaaaattaa
    28501 ctcaacctga aaagattatg aatcgtccag acgacaaaac atgccatgac ttttatgcaa
    28561 attcttatgc caatgttttg ctttgcagcg cttcccagtc tatagataat ctttcaatca
    28621 ttattattat cttgccctct ttcccctaga gcggtcagct gttgctaatt ctctgcctct
    28681 ctagggaggc ttccaaagag ttagcaccaa ctgatttcaa ctggagggcg tcatgaatca
    28741 aaatagtttc aaaccaaaga ataatttcgt ctcattggtg atgtgggcat tacctttatc
    28801 tttttttact tttcaattct tattacgtct ttggcctgga ttaaccatgc aagacattat
    28861 ggcgcaattt tcaattgatg ccgcaagctt tggccttatc gctgcattct attattatgg
    28921 atacgcagga atgcaaatcc ctgtagcctt actgctggat aaatttggag cccgcttcgt
    28981 tgtatttgcc tttgcaatgt tgtgtggtat tggaacttta gttttcatag gtaccaataa
    29041 tttttacttg gctctgtcag gcaggttttt gataggtgta ggctctgcgg ctggttttct
    29101 tggcgtatcc aaagtcgtat cagagtggtt cagccattca cagtactcca gaatgatcgg
    29161 tttctccttt agcgtaggct tgttgggggc aatctatgga ggaaaaccgc ttgcccacct
    29221 gatacagaat tttagtggac aacaagttgg tttggctttg gcggttttct ccatcctctt
    29281 aggatgttgc gcacttttga tcttgcgctc tcccccaaat aatactcact cagtaacaaa
    29341 aacaatgaat ttgtctcact gttcctcttt attttcatcg tggatcatct gggctcttgc
    29401 tcttgctaat ttattaatgg tcggtgccct ggagggattc gcggacgtat ggggagtacc
    29461 ttatcttatc gccaaatacg cactgaacaa aactgatgct gcttttctca tctcgtttat
    29521 ctttatgggg atgatatttg gcggtccttt actcgctttc ctcagccaaa aattaggaaa
    29581 ctactttgta atagccctct gtggtactgg tttggccttc ttgttcctaa tccttcttta
    29641 cactgctaca cttcactggt ggcagctggg cactctcttc tttgcaatcg gcctgctgtg
    29701 ttgctatcaa gttattgtat tcgccgcagg ttctactttg gttccttatg aaggtttagg
    29761 cgtcaccatc gccttcttga actgcatcaa tatgttaggc gggtcttttt tccatacttt
    29821 gattggtaaa ttaatggata gcagttggag cgggcaaatg agcgctaatg gacttaaatt
    29881 atatgaagtg gaatcgtatc aatgggcttt gtcactcatt ccattctgtg ccttatgcgg
    29941 cagtttgctt attgtattga ttcatatcaa aacaagagct tatgcagctc cagagcttat
    30001 gaaaaagact tcattgacgg gagtgtagct atggaaaaaa ttttttggtc taatccctac
    30061 cagcatacac tcgataccaa gataatctca attaatggca acgagatcct attggaaaaa
    30121 accatcgcgt tttcattttc aggaggacag gaaagcgata gagcattcat caatggcctt
    30181 gaagtactca gttcacgaat agatggaaat cttatttatt ataccctgcc tgatgaccat
    30241 aatcttgcgc ccaatgatat aatcaccatg actatagatt ggcctcgaag acatcggctg
    30301 atgcgtcttc attttgcagc agaactgatt ctggaacttg taaccagaac cagtatgctt
    30361 gaaaaagtag gagctcacat ctcggaatcc aaagctcgaa tagattttaa agcgaatttt
    30421 aatatctctt ctatttttag ccagctgctt gatgaatata atgccatcat tagacaagat
    30481 cttgttatcc ataaaggata ccatgatgtg acaaatcaac gtcggttttg gaaaattgac
    30541 ggttttgctg aagtgccttg tgggggaaca catgttcact ccacttctga agtgggtttt
    30601 attcgtctca aacgctcgca tcctggcaaa gctattgaac gcattgaaat aaccctgatg
    30661 gatgatagta gataatgcac ccctcgcata agctgattgt tttgctttgg ggcaaggtga
    30721 gaatgttatg gggcaatgag tttatataca ttgaatgaac gttgctgaaa cgtttggagc
    30781 gtgacagtaa aataaacaag gcaggttatt caggagacca ggtcaagcgc taatcgagac
    30841 aaaaatatag aaaaagaata caaaatcgcc taaattacag catttgactt tgtcgccatt
    30901 aacctgtgag cgttatttat catggaactg ccctatcatt tctctactgt ctatgtgtca
    30961 aaaccagagt ttatagaagc actttctgag gagttaggcg aggtatccaa taaaataggc
    31021 tctctcgttt tttctcctca tcagaagctt gatgtttgtt ttgcactgga tgtatggctt
    31081 gaacctgaga tagtaaccat tcaatccatt tcagaagcag caaaaatatt gaggcaagcc
    31141 ggaaaattct ggtatttgca ccctgttact gaaattcgcc gatcgcgctt gattgaggaa
    31201 caacttcgca agtgcccatc actaatgtcc cattttccag ttacaagtga cattccaccc
    31261 ataggtgtat ttagtcttct tgatagaaac actctgctct ttagcgccaa acgactaaaa
    31321 aaatggccgg atggacaatg ttattttatt gaagacaaag tcaatccccc aaaccgggct
    31381 tatttaaaat tatgggaagc cttgttcctg ttaaataaat atcctctccc tggtgaaaca
    31441 gttttagatt tgggcgcatc cccgggaggt tggacatatg tgatgcaatc actcggaacc
    31501 caagtcactt cggtcgataa agcagaactg gatacaaaaa tcatggcgct tcccagagtc
    31561 agttttttaa agcaaagcgc ctttgcctta gatcccgaac aactcacaga gtcttatgac
    31621 tgggtacttt ccgatgttgc ctgctatcct gacagagcct acaccctcat caaaaaatgg
    31681 atagcatcaa aaaaagcaaa acagttgatt tttaccatca aactacaggg acaaactgac
    31741 ttaaacacaa tcaaacgatt tcaatccata cctaattcca aagtaatcaa tctgttttat
    31801 aacaaacatg aagtcacgtt tttttatcca gtataaacat aagcaggatt tagtgattat
    31861 gtctgcaaaa atatcataat gacgagctaa tatttaattt tcacgactta agatcttgaa
    31921 aaatcaaaag aaaagacatt ggcaatgttt ggttgtttta aggcgagcgc cagcaatcga
    31981 tcaaccccca gggcaacccc gctgcaaggt ggtaacccat attccagggc tttcaaaaga
    32041 tatgcatcgg cttccattgc cgtcaatccc ttttgctttc tatgttgctg atccagcata
    32101 aaacgctttt tttgcgcctc gacatcggtc aactcatgaa aaccattagc caattcaact
    32161 ccctgatagt acacttcaaa gcgctcagcc aagccctctt ttacttgtgc caaggcagcc
    32221 tgggaggcag ggaagtgata cacagcaacg ggggttgatt cttctgccaa aaaaggctcc
    32281 acgacatggc tcattaataa aaacagatat tgatccctgt cttgttcgtt aggagataag
    32341 acaccatcca aatcatgctg tgctaatacc ttgttgaatt gttctatgga tgtagaaaaa
    32401 gggttaatgc cgcaagcctc ttcaaaagcc tgttgatagg tttttctgat cataggccgg
    32461 gatttaagaa taatttgtaa taataaatcc acttcatcca tcaaaacatg atgatcaata
    32521 tgtaattgat accattcaag caaagtaaat tcaggattat gccagcgccc cagctcgtca
    32581 tcacgaaaaa ctcgcgctaa ttgaaaaata ggcccgctgc cagcagctaa cagtcgtttc
    32641 atatgatatt ccggggaagt ttgtaagtag catgtttcac ctctgaaaat cgctttgata
    32701 ttgctcaggt aaacatcagt aaccccatgc cgcgccatca caggggtttc cacttccaga
    32761 tattggcgtt cagtaaaaaa ttgacgaatt ttacttatta gctcggctcg cgcacgtaat
    32821 aattgaattg aagctgaggg caaccaggag ttatcagaca tgatcaataa aatattccca
    32881 actccaggcg ggcagcctct gtcatgcggt cctgagacca gggcggctcc cacaccaact
    32941 caacagtgca atcactgact ccttcaacct gattgactgc ctgctccacc gtaccgggaa
    33001 aggtttgcgc cacagggcac cccggagtcg ttagcgtcat ctgaatatga acatggcctt
    33061 cgtcattaac ggcaatgtca taaatcaagc ccagatcata aatattgacc ggaatttcgg
    33121 gatcaaaaac tcccctcaac gcggtaataa cgccttcctt tattgattcg ttactctgtt
    33181 ttttcttaaa accaaacatg agctgcctct ggtctatttt gcaatataca gacaaactct
    33241 ttcacgagcc ctgccactat tgccataaca ttattctgtt ttcactactt ctgtttcttt
    33301 gtttaaagcc gcttccaaag tatgccacgc caaagtggcg cattttacac gcgcagggaa
    33361 tgctttaact ccggctagaa ctgttaattt atccatggaa accaaagaat cttcttcctc
    33421 ttgtgttagc attcgatgaa cacgatgaaa tagctcatga gcttctttaa ttgatttacc
    33481 ctttaacgca tcagtcatta acgatgcaga agcttgtgag atagcacagc cacatccaac
    33541 aaaacttaca tcacggatca aatcaccctg caacttgagg taaacagtca gtttatctcc
    33601 gcataaaggg ttaaaccctt tcgcctcggt ggtcgcatcc tccatcgcat ggtggttacg
    33661 aggattacga ttgtgatcga taatgatttc ctggtaaagt tcccgcaact ccatactcat
    33721 gcgaatacct ctttaactcg ttgtaaggct tccatacaat aatcaatttc tttaaacgta
    33781 ttataaaagg acatggaaat acgagaggtc gcagcaacat cgtaaaaatc catcaaaggc
    33841 atagtgcagt gatgtccgct gcgtatcgct ataccttcac tatccagaat agttccaatg
    33901 tcatgggcat gaatttttcc gtggacaaaa gagataatgg gtactttgtt ggcagccgtt
    33961 ccaataatat tgtacccttt tactgcttca atagccttgg tagcatagtt taataattgt
    34021 gtttcgtatt cagctatcgc atctaaatcc aatgaccaaa tataatcaat tgcagcagct
    34081 aaaccaatcg cccctgcaat attcggggta cctgcttcaa atttatgggg gatagctgca
    34141 tattccgttg cttcaaaact cacggagttg atcatttcgc ccccgccctg ataaggagtc
    34201 atgctattta ataattcttc ttttccccat aaaacaccta ttccagtggg accatacatt
    34261 ttatgtccgg aaaaagcata gaaatcgcaa tccagatcct gaacgtccac aatcaggtga
    34321 gccgttgcct gtgccccatc cagtaacact ttagccccta cttcatgggc catcttaatc
    34381 attgttttaa caggattaat ggttcctaaa gaattggagg cgtaattaat cgcaaccatt
    34441 ttagtgtttt catttaactt tctttcaaac tcttcaagta ttacctcgcc atttaaagaa
    34501 ataggggcga cttgcagctt gcaacccatt ttcttgcaga ccatttgcca gggaactata
    34561 ttggaatgat gttccatatg cgttatcaaa atttcttcat caggtaatat gcgaggggct
    34621 acgagactct gagccactaa attaatggct tcagtggtac cacgtacaaa aatacattct
    34681 ctcggtgacc tggcattaat aaatcgtttt acctttgctc tggctgcctc atacatttcc
    34741 gtagccctga cactcaaggc atgaactcca cgatgtacgt ttgaattatc cttttcataa
    34801 aattgagcaa tagcatcaat cacagctttg ggtttttgag tagtagccgc gttatcaaaa
    34861 tacaccaaat catactcatt gattttctga tgcaatacag gaaaatcttt cctgattttg
    34921 ttgacatcaa aggatgtcat tagggaagga ttagtactca tttgcgtacc ccaactgttg
    34981 agttaataaa ttacccatcc aatcagccaa atcacgattt ggaataaggc gcaaattatc
    35041 attggcaaat gcctggatca aataatgaga ggcttcatcc cggctaatac cacgtgttgc
    35101 caggtaaaat attgcctctt catccagttg cccaaccgta gcgccatgtg aacataacac
    35161 gtcatcggcg aaaatctcta attgcggttt ggtatcaatt tcagcatttg cggagagaag
    35221 taaattttta ttttgttgct tggcatcagt gtgttgcgca ttcttggcta caataacctt
    35281 gccattaaat accgctctgg aatgtccctt taaaatccct ttgtaatctt gatcgcttcg
    35341 acaattaggc accaaatgat gaaccacagt atgatgatct atgtgttgtc catcccccgg
    35401 tgcgtaaatt ccattcataa ggcattgagc ttgttcttcc tgcatggaca cgctaatatc
    35461 actgcgtact aacttcccac ccaaactcaa agagtggctg gaaaattgac tggcggcaga
    35521 ttgcctaacc gataaatgac ctaaatggta agctgattta ctttcttttt gaattttata
    35581 atgattaagt tttgctcctg ctccaagaaa cacttcagta atactgttag tgaaatagca
    35641 aatgttttct tcgccttgat attcttcaat cagagtagcc tgagattctt tttccataag
    35701 gatcagatgc cttaaatgca tagcctggtt cgtttgatcc tggatatgct ttaaaacaat
    35761 gggttcttcc aaacaaattc ccgcagggat ataaataaag actccgcatt ggagcatggc
    35821 tgtatttaga taatgaaacc catgctcctg ttctacaatt tttccaaggt aaggcttaac
    35881 caaatcagca taatgtatta aagcaagaga taaaggcaga attaacacgc cttcaggcag
    35941 attcttaatc cattcttgct cttgggaaat aaatccattc tgtattaaaa acaggttttt
    36001 gactggtgaa tcgtcaaagg cggttttaag agcaggcttt actgtaggat caggtagtac
    36061 aaaatgttgc tgtaatagtg agtcaacgag agtgtacttc cattcctcat catatcgggt
    36121 gggaaaacca cggcgcttca aatcagttaa tgctttagac tgcaactgcg caagccaggg
    36181 tatttgcgat acgcctgatt gggcttgctg ttgataataa tccaaaatat cactcataac
    36241 tgctccactt cttcaagcca gctataaccc tttttctcca gttccagcgc caaagattta
    36301 tcccctgacg taataatacg accatttgcc aagacatgaa taaaatcagg ctcaatataa
    36361 tccagtaagc gctgataatg ggtaactaaa ataatagaac gctcgggaga gcgcatcgca
    36421 ttaactcctt gagaaataat tcgtaatgcg tcgatatcca ggccagagtc ggtttcatca
    36481 agaatagcta gcctgggctc caaagctgcc atttgcaata tttcattacg tttcttttcc
    36541 ccacctgaaa atccttcatt aatacttcgg tataaaaagc tttcatccat atccagaagc
    36601 tgacattttt cccgaataaa acttaaaaac tctatggcat caagggtgtt tcttccttgt
    36661 cctttgcgaa ccgcattcac tgaggcttta agaaaattga tattggttac accaggaatt
    36721 tctaccggat attgaaaaga cataaaaata ccggcccgcg ctctctcttc cggggacata
    36781 ggcaacaaat cttgtcccaa ataggatata tcaccacgag ttacttcata agaaggatgc
    36841 ccagccaata ccttggagag agtacttttt cctgaaccat taggtcccat aatcgcatga
    36901 acctctcctg gtttaacctc aagattaata ccttttaaaa tagactgtcc attgacagca
    36961 acattgagtt ctttaatgtt taacatatta gccgactgcc ccttctaagc ttatacctag
    37021 taatttagtg gcttccaccg caaattccat aggtaattct tttaaaactt gcttacaaaa
    37081 tccattcacg atcatagaaa ccgcatcttc tgtatcgata ccgcgttgct ggcaataaaa
    37141 caattgctct tcactaattt ttgaagtagt ggcctcatgc tctacttgtg ccgttggatt
    37201 tttgacttct atataaggaa aagtatgtgc tgaacattga ctacccatta acatggagtc
    37261 gcattgggta taattacgcg cgttcgtggc tgttggtgcg atgcgtacca atcctcgata
    37321 agcattatgc gcatgcccgg cactgatccc tttcgaaata attgttgatc gggtattttt
    37381 ccctaaatga atcattttcg tacctgtgtc agcttgctga aaattattgg ttaaggcaac
    37441 agaataaaac tcgccaacag aatcatcacc ttgcaaaatc acactggggt atttccacgt
    37501 aatggctgaa cctgtttcaa tttgtgtcca ggaaattttc gatttcttac cccgacaagc
    37561 tcctcgtttg gtcacaaaat tgtatatccc ccctttacct tctttatcac cgggatacca
    37621 gttttgtacc gttgaatatt ttatttgagc cccatctaac gcaatcaatt caacgactgc
    37681 ggcatgcaac tggttttcat ctcgcatcgg ggcagtacac ccttccaggt aggacacata
    37741 actgtcaaca tcagcaataa taagtgttct ctcaaattgc ccggtagaag cagcattgat
    37801 cctgaaatag gtcgatagct ccatgggaca tcggacacct ttagggacat aaacaaatga
    37861 tccatcacta aacacagcag agttcaaagc agcataaaaa ttatccctgt aaggaacaac
    37921 tgaccccaga tatttcttaa ccaagtccgg atattgatgt accgcttccg ataatgggca
    37981 aaaaattact cctttttcag ctaatttcgc tttaaaagta gtcgcaactg aaacactgtc
    38041 aaacaccgca tcaacggcaa cacctgccag catttcctgt tcgcgtaaag gaatacctaa
    38101 tttctcatag gtttttaaca actccggatc aacttcatcc agacttttgg gagcgtcttt
    38161 tttactttta ggcgcggaat aataggaaac ggactgataa tcaattggag gatagtgcac
    38221 actcgaccat tcggggtgag gcatagtgag ccagtgccga aatgctttca accgccactc
    38281 aagcaaaaat tcaggttcgc cttttattgc agacaaacga cgaatgacat cctcatccaa
    38341 tccgggagga aaagtttcaa cttctatgtc ggttataaaa ccatgttgat attctctatc
    38401 gagcagagaa tttagttgct cactgctttt agccacgatt tactccactt gctaattgcc
    38461 gaactcgctc aacatccctt gttggaagag ttggttttgc caatgcctcc aaactgacac
    38521 tatcaagagc agtttctatc gcatggctta ttaatcgcca atttccttga atggtacata
    38581 ctccttgtaa aaaacaatca ttgggttgca tactgcattc tgtgaaaccc ctgtgctctt
    38641 ccaaagcaaa tatgatttga gacaccgaaa tttcagctgc tggtctttgc agtctatatc
    38701 ctccggatac acctcgtacc gaagttaaca aacccgcgct agtcaggcgt tttagtattt
    38761 tactaacagt aggtaccgtt aaatgcgtat gtaaggcaat atctcgagca ttgcataatt
    38821 cctgcgaatg cttggctaga taaaccatta caactgttcc ataatcggcc aatttactga
    38881 cgcgcagcat aacaccccct actcgaataa tctagtacca aataagtctt attttcagat
    38941 agaggaaaag aatatcttat taagaacaag gattagacaa ttgcttactg atccaatatc
    39001 acctgatcct ctaagtgagc ttttctggca caatcatcca aagcaaaatt ctattacctt
    39061 attataaata tagtactaat ttggttctat attcgataaa agagagattt taccttattg
    39121 ataaggaaat aagcaaataa aatcttctgt tcgatcttgg cgatttcatg cgttttaata
    39181 ttttttacaa taggttagtc ataaagggaa tgggcttgcc aatattccat ctgtctataa
    39241 tgtgccctgg atttaatata gaatactctt gataaacaca accaaaccca taagaataag
    39301 ctattcgatt atttgctttc aggagccagg atgactcacg atactgaact aagcgaactg
    39361 atgcaggctg tagctaaaaa aagcctgcag attatgaccg atttcaaaga aaaacccatc
    39421 ccgatttcat cgctggtcag tcaatacatc gatctcacgg agcatttcca aaatttgatt
    39481 gccgtcattc taaaaaatcc tgaaaaagta tggcaaatgc agttgaatta cctcgaagat
    39541 gccttaagtc tggctcaagc acaatttaat tattggcttg aaggaaaacc actgcctatt
    39601 aacgatcagc gttttaatgg ggaagattgg ataaacaatc ctttttttaa cttgctaagc
    39661 caacattatc ttttggcaaa tgagcatatg aattcattgc tggaaaatat ggaatatggt
    39721 gacgagaatc tggcaaaaag agtacgtttt tttacccggc aatatcttga tgctttatcc
    39781 ccagctaact ttattcatac gaatcctcag ctcatggcag aaacattaca aagccatggc
    39841 aaaaacctat tacgtggatt acataatttg ctgtccgatg ttgaagcagg atcttccaga
    39901 ttaatcatta aaatgactga tacagaagca tttaaaatcg gtgagaatct ggctacaacg
    39961 cctggtaaag tcatatttcg caattccatg atggagttaa tccaatattg ccccagaaca
    40021 acaaaagtaa aatccatacc tttacttatc gttcctccct ggattaataa atactacatt
    40081 ttagacttaa gcccgcataa ttcattaata cgctggttgg tcgaacaagg gattactgtt
    40141 tttattatct cctgggtcaa tcctgacgaa acgtatgcca ataaaagctt ctatgattat
    40201 ttaaatgaag gccccaggga agccatttca gtaatccaaa aacaattgcg tgttaaacaa
    40261 gtcaatacgc tgggattctg tataggcggg acacttctgg cttctctttt agcctacaat
    40321 aaggcaacta aggatctttc cattcgcagt gcaacctttt tagcggcaat gattgatttc
    40381 agtgatcctg gagacatcgc tgtttttatt gatgagcagc agattaataa acttgaagaa
    40441 gaaatgaaat ccaaaggtta tctggctggc aaatttatgg cgtccagctt taattcctta
    40501 agggcaaatg acctaatatg gtcatttttc atcaaaaatt acctgcgcgg taaaagccct
    40561 gttccttttg acatactcta ttggaatgct gattcaacta atatgcctgc aaccatgcat
    40621 tctcaatatt tacgatggat gtatttgcat aatgacttga taaaacctgg aaaaattcgc
    40681 cttaatcata cccctattga cgtaacaaat atcgacatac ccactttctt tctgtctact
    40741 caaaaagatc atatcgctcc ctggaaaaca acatataaag gatttgaatt aatgaagggg
    40801 ccaaaacgtt ttgttttagg tggatctggc catattgcag gaattatcaa tccgccaacc
    40861 cagcaaaaat atggttatag aaccaataac agcatggatt tatctgctga gcaatggttt
    40921 gaaaaatcca aggaacactc cggttcatgg tggccagaat ggttaaattg gcttaaattg
    40981 cattccggaa gactcatcaa ctcccctgat atcaatcact tgccattcgc acctataatg
    41041 gatgccccgg ggagttatgt actaaaaaaa taatattttg tactcaataa ccatacatta
    41101 aaacataaaa ggagttgtat tgtgagaaaa aaaaacagga tacttttggc aagtttattc
    41161 tcgctaggca gcctgaatgc ctttgctgct caagatctca cggttgttaa tacgtccgat
    41221 gatctgggat atgtgacaat aggatcctat tcctgtgaat tgacttccag ttgtgctatt
    41281 ccccctgata attcgattcg tattaactct gatatcgtag aagcagcttg tgcgaatacc
    41341 cccgctgatt gtgaaattgt ttttggggtt gacagtataa aagcaagaat cgcgaccgga
    41401 agctttagtt tgaatgaggg gatacaatac atcgcccaat ctcgttggct gaattattct
    41461 gctaagcgtt tatccaaaac cgtggctgaa atctataaaa gataattcga tttatttcct
    41521 gctgatgtcc tggttgaagc acacaacaat aaccaggaca aggtgcattg acatggattg
    41581 attaagataa atcataaaca cagggatctg attttctcca ccagctctct gttcttgcca
    41641 aaatcagaat accccatggt ggcacttgaa ataacattcc catccgcatg gatgcatatc
    41701 caatcaacaa agtggaaaaa ggggctttgt ctatacccca ccagaaaaga tgcctcctgt
    41761 ttctggattt tcaactccgg gatcctgcgt tcaatacatg ctaccaattg aggcatactc
    41821 tcaaccttca aaggggcaat atagtgagaa tccgattcat taaccaggct acttacccag
    41881 tttggcttat catctggcaa ttgcccctca actaacccct tcggcatttc tcgaaaattg
    41941 atttgaggaa ataaataaag aatgacaaca agggctaaaa taagggctaa tactatccat
    42001 attaatttca tttatactgt ttccatggtt tctttctgtt ctaaaaattg cttttcaagc
    42061 gttatataat caatttttcc tgaaggcagg acaggaattt ggcatgccgg ataaatcata
    42121 tgaggaacca gcaattctga ttgccctctc tccttaacct tttttataaa tgatacttta
    42181 tctgcatact ctgcttcagt aaataagaaa atttgctccc cctttcttgg agatttttgc
    42241 acaacagcgg catgtaataa ttcaggccaa attgaagctg caattccttc caccgctgta
    42301 agagatacca tttcaccagc aatcttggca aaacgtttgg cgcgacctgc tatagtaatg
    42361 aatccatctg cattcacggt gacgatatct cccgtatcat gccaaccatc ttcaggttct
    42421 ataattactc caggtttatc ctcatcaaga taacctaaca tgatattggg tccgcataat
    42481 tttaaacgcc cgccttgtac aattccctct actggttcta ctttgaaatc cataaaaggt
    42541 aagatcattc cgacactacc cggtactgat gccaacggac aattaagcga tatgactggc
    42601 gatgcctcag tagcaccata gccttcataa attcttgccc caaacgcttc gctccagtat
    42661 ctaatggttt ccggtttcac tttctcggcg ccagcgaaga tatagcggac gctgttgaaa
    42721 tcatgtttgc ccgcggccct tgcataaccc gttaaaaaag tatctgtgcc aaacaaaata
    42781 gtagcccctg tttgataaac caatccagga attaccttat aatgaagagg ggaagggtag
    42841 aaaaaacaat tcaaaccatt aaccaaagga agcacacttc ctgctgtaag gccaaaacaa
    42901 tggaatatag gtaaggaatt aaataaaaca tctcgtggag taaagtcgac tctggaagtc
    42961 atttgccaac aattagccaa tatattggca tggctaagag caaccccttt gggttttccc
    43021 tcagaaccag aggtaaacag gattatgcct gttgtatcag gagatatttt gtcaccaaga
    43081 aaacggtagc ttagagtggg aaagaagcct ttgaatatcc cggaaagctt gttacctaaa
    43141 tgaatagttg atttaaaatc ctctaaataa tgaattctta tacccgcctg ttgcaactct
    43201 tctaccaagg gttccaattt ggcggtttct atgaattgcc ttgcagtata aattgattta
    43261 attcttgctg tattacaggc tgaatataag ttataaaaac ccatgctaaa attcaacatg
    43321 gcaggtattc gtctataagc ttgtaaagca aaaaaagtga ccatgccagc tatagtagtc
    43381 ggcatcatta ctccgaccgt ttcgcctata gccgtttgct ttttaatttg gcgccccaga
    43441 ataaaacaac gagtcaagaa ctgtctgtaa gttaagggaa tacgattatt atcttctatt
    43501 ttagttttgt ttttagttcc tatacttact ccttcaatta aagcagaaaa taaaacatat
    43561 ggcttaaaac tattggcgaa tgacaaatca cttattagtt gaaataaacg taaactgata
    43621 ccttctctgt cgacacctgt attttcagat tgactcaatt tttgagcagg taatatatga
    43681 agagtaattt tcggaaaaat ccgtattaga tatttatttt tgctattaga aaaaatactg
    43741 tgctccgccc cttcaatacg aacagggatt acctcggcgc caaccttttg gagtatcaat
    43801 ccaggtcctg caaaggcctt taagctgtct tcctgctgtc ttaataaccc ttgtggaaat
    43861 agaagacaac gcttgccagc ccgtattgcc cttattaaaa ttcttgttgc ctgagcgctg
    43921 cctggatcaa ctactatgac atcagcaaaa aataataacg tttttaccca tattttttta
    43981 aacatatcag gatgtaaagc taaagttaat cgttcaggta aaaatacaga aaggagtaac
    44041 acatcaatta tagaagccct gttggcaata atgacagaat ggctattggc ctgatattgt
    44101 cctttaacct ctatgcgaaa ccatttctcc agaaaaattt ttatccatct tctggcccgc
    44161 tgtttttgtt ttcccttcca ggtcaaattt tcctcatttg tttttgtcat catgtcgcat
    44221 cagttatctc tatattgact tttattctgc aagattcaaa acgatttgaa tagataggtt
    44281 tatgaatcat gttgatttca tttaaaaaat ttaagcgaaa gcaacttctg tttttatgat
    44341 aatttaccca gaatattgag agaaggcttg cgagtcctta attcctgtaa taaagtgagg
    44401 ggcatcttta ctttaacaca aaaaaccatt aagacattct gtttcatttt agccatatta
    44461 gttttaatta ttttcgcagt ggtctttcat aatcattcgt tagagatcat tgattatata
    44521 gacgatctgg gttggcttgc ccctgttctt ttcttgatca cctactgcct cgcgacttta
    44581 ttgcttttac ccacaatggt attaactctg gctggaggag ctgtgtttgg gccagtattt
    44641 ggtaccttgc taaatttatt gggagccact agcggcgctg cgtttgcatt tctgataaca
    44701 agacacctgg tttacgattg gttctcaacc aaaaagggtg aaaaactaaa caaattgatt
    44761 gccggagtag atgagaaagg ttgggttttt gtggctttct tgcgattgtt tccaatagtc
    44821 ccttttaacc tggtcaacta tggattagga gtaactggaa tatcatttcg tctttatttg
    44881 ctaactactt ttatttttct aattcctgca gaaattattt atacctattt tggatacgtc
    44941 ggcatggatg ctctgacaag gccaggccat ttatatagga atggtggagt ttttctaact
    45001 ggattagcga ttttcttttt acttgttatc agaataatga aaaaaagata gttgtttttt
    45061 caattaataa aaatttatca agactcatcc tgatttaata aaaagatcaa cttatcgata
    45121 attggatgat tgacttcaaa aataatgtta tccagggaga gcacccttct gataccctgc
    45181 agactattag ttaaaaaaac agcatcggcg tcctcaatcc tctttttcgt taatgatatt
    45241 tcctgcactg acattttgtg ttgctgacaa tgtgaaatga ggcgtgccct ggtaatgccg
    45301 ggtaagatcc catcctctat tcgcggggta tacaaaatgt tattttcaat caagaataaa
    45361 ttggcgcaag ttgtttcagt aacatgattc tctgtgttga aaaataaggc atcgtctgcc
    45421 ccaaccgcga tcgcttgacg ttgagcaata atcgcctcta aataattcac tgattttaat
    45481 tgataaagag gattggcctt atcccttagc caattaatac tgattaaccg aacaggatgc
    45541 ttttgtattg aataattgaa agtctgaaat attaattggc tgacttgccc tcgttctgcc
    45601 aagcctctcg aagcaggacc accactcaaa atggctttaa tgccaccatg gtacaggtta
    45661 tctttttgga ttttttgaat aagatgctca aaccaatcat caaatgaaat ttcaaaagga
    45721 atacccaact gacgagctga attgcctagt ctctcccaat gcatataagc aaaggaaggc
    45781 ttggaactat tgactctaat tgtttcaaat aatccctcac ctaaaaaaat tcgatcatca
    45841 attccaaatg atggcgtcat atctcctttg tcttcaataa cacgagtagg cattatgatc
    45901 tcataattaa taatctacaa aatcatccaa ctcatcccgc aatcgctttt cctccaacat
    45961 attttccagt cgtctgcgcg catccaaact gacaccggga gcttctataa tttcagtatc
    46021 cacttcaaca tcatcaaaat cgtcgacaac ttcaattact tcttcttcat cttcttcaaa
    46081 caagtcactc ataacattcc ctcaaagaat taaaagtaac attaaataat tgcttaaatg
    46141 gaacaagatt ccaggacggc tatatacctt atttttaatt ttttgcatag tattttttaa
    46201 ttattttatg ttataaactt atcgcatcgt gcatttacaa tcacccaatg cataaaacaa
    46261 catattgata cctggaaaca cttgaacttt aagcccaatc catctaataa taaaaaagaa
    46321 tattagtgat accaaatcat gtcagaccaa tataaagttg ctctaagaaa taccataaaa
    46381 caggtaagat ccaagctttc tgtatcctac agaacagcat cttccaatca aatttgtact
    46441 cgtatccgct cgctggaaca atacaggcaa gcaaagcgaa tagccttgta ctttgccgtt
    46501 aatggagaag ttgatttaac atttctgtgg aaaagcgcgc ccctccaggg gaagtattgt
    46561 tattttccgg tattaaataa tgattccact ctttcttttt taccagccac ccccaacacc
    46621 ccttttaaaa cgaataaata cgatatactg gaaccggacg ttgatattga gttggccttg
    46681 cctgttgatg aattagactt gattatcatg cctctggttg cttttgatgc gcactgtact
    46741 cgtttgggta tgggctcagg ctactatgat cgcaccttgg ctaataaatc caaagcgcat
    46801 ctcttaggag tcgcctacca atttcaaagg gttgattaca tagaaccacg cccttgggat
    46861 gttcccctgg atgccgtaat aactcaaaaa gctatttatt ggcgaaaccc cttggcctaa
    46921 aatgacattc tatagagcgt ctgtaccgac ttcccctgtt ctaaccctca ctacctgttc
    46981 caaagcataa acaaaaatct tgccatcacc aattttacca gtgtaggcgg acttgcaaat
    47041 agcttctatg gctgcttcaa ccatattatc tggcaatgcc agttcaattt taattttagg
    47101 gaggaaatct accacatact ccgcacctcg atataattcg gtatgacctt tttgacgccc
    47161 aaatcctcga gtttcagata tggtaattcc tggcactcct atttccatta aagcttcatg
    47221 tacatcatcc agtttaaatg gtttgataat cgcagtgatc attttcatat ttttttccat
    47281 cacgttaaat gcagagccac ccatgatatt tctaacacac aatttttatg ttattgcttt
    47341 tcaagacatc ttgggtatag ttgtaatcca acagttttgc tagactgtat tcaaaaataa
    47401 tccggagcca ccatgtttga ccccaagcaa tttgatgaat tagctaataa gcttttcgct
    47461 actttaccta cgagtctaca aaattttgaa aaagacattc aacaaaaatt taaagaagta
    47521 ctgcaatcaa cattcagccg aatggacctt gttacccgtg aagaatttga tgtgcaatgt
    47581 aaagtacttg ccagaactcg agaaaaactg gagcaattgc agcaccagct tgaagacttc
    47641 attaaacggc aagaaattaa aaaataataa ataataacta ttagtcgtcc ataggaaatt
    47701 ggattgactt aagtccaaca taataaggaa aaaatcttat gaatctcgca tttaccaaaa
    47761 cgcgaagtac tattggtatt gtcgcacaac cagtatctgt ggaagtccat ttatcaaatg
    47821 gattgccaag ttttactatg gtaggacttg ctgaaacagc agtgaaagaa agtaaagacc
    47881 gcgtgcgcag cgccattata aatagccaat tcgaatttcc atgccgtaaa attaccgtta
    47941 atcttggccc tgccaattta cctaaaacgg gaagtggctt tgacttgcct attgcactag
    48001 ggatactcgc agcatcagaa caaattcctc tcaccaacct tgcaaatcac gaatttattg
    48061 gagaattggc tttaagcggc gagcttagag gggtttctgc cattatacct gctgttcttg
    48121 ccgctcataa agacaatcaa catcttatta tagcgaatgc caatgctgcc gaagcttctt
    48181 taaccggcca cccaaaagtc tttactgcca ataacttacg cgaagtttgt gattatctat
    48241 gccagggtac ttctttacag aacttacctc ccaaaccaga tcttttatta aataaccatg
    48301 aattggattg gtctgatatt aaggggcaac atcatgccaa aaatgctatg gtcattgctg
    48361 cctgtggggg acacagcatt ctgctaagtg gcgcgccggg cagtggaaaa acgatgatgg
    48421 caaaaagatt tagtaccttg ctacccgagc tatccgaaac acaagctctc gaatgcgcag
    48481 ccattaattc aattcgaggc aaattacctg atttcaggga atggcgtttg cctccttttc
    48541 gtgcaccaca tcatactgct tcacctgtcg cattagtcgg aggaggcaac ccgccgaaac
    48601 ccggtgaaat ttcattagcc catcacggcg tattattttt agatgaatta cctgaattta
    48661 atcggcaagt actggagaca cttagagaac ctttggagtc agggcatatt tgcatttcca
    48721 gggcagctgc acaaatagag tttcctgcca aatttcaatt aattgcagca atgaatcctt
    48781 gcccttgtgg ccaatggggt aattcacaag ctaattgctt atgtactcct gatagaatca
    48841 gccgctattt ggcaaaactt tctgctccat tattagatag aatcgatatg caggttacaa
    48901 ttcacgcatt atctcaagag gagttaatta aacccaatac ccatttagaa aaacaaagtc
    48961 ctgctattag agaaaaggta acaaagatgc atgaaataca aatggcacga caagatagtc
    49021 tcaatgccaa tttaaattcc aaaacatgcg aaatggtttg cgaattggga tcagaagaac
    49081 aattgtttct ccgagaagtg atgagtaaac tgaaactgtc tgctcgtggt taccatcgct
    49141 tactaaaagt aagcaggaca attgctgata tgagtgaatg taataaggtc atgctaaacc
    49201 atctgcaaca ggcgttatcc tataaacaaa tcttgcattt gcctaaataa aatttcttct
    49261 caagtattgg ggtgtataaa aaacaattcc gtattaatca tatacaccaa agttgtccta
    49321 taattataca taaatataaa ttacggggcc atcatgagaa gcataagact cttgtttcct
    49381 tgcattttat taatgatacc atggcaattt gcaacagcta atagacctac tgataatgcg
    49441 ctagcgatta taaaatcatg ctatgatgaa tcagtaaacc tcagcaatga ctttactccc
    49501 tgcgttacca aaaaactcaa gactatgcct aacgcagagg attttcaatt gattatgaat
    49561 tttaaaactg acgaaaaagg cacgaatgaa cgaataccag tctttatggt cgataaaaca
    49621 gggtatatgt attactgtat agtaaccaca ggaaaaactt taatcataaa tgcttgtgcc
    49681 ggagaacagg gaaaaccgct gacgcaaggt caaatgttat ccatcgatct acctgaataa
    49741 ttttgcggtt tcctatctct tgcgaagaat ggctatccat gagatcttcg aatatagata
    49801 aaatgcataa tcaagcagaa aaaattatat tactcgcgag atacacatat tcgaatttat
    49861 caaattaaaa gagaaaaagt taaataaaaa tttaaaaagt tattatccaa tatctcccaa
    49921 acaattctat aatcaatcat atagattaaa taaaatacaa tatatgcgaa tatcaagact
    49981 attctattta ttttttttag tatttt
//
LOCUS       DATNSN010000007        48056 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.007, whole genome shotgun sequence.
ACCESSION   DATNSN010000007 DATNSN010000000
VERSION     DATNSN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 48056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48056
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.007"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(680..1396)
                     /locus_tag="VAH76_000321"
     CDS             complement(680..1396)
                     /locus_tag="VAH76_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEO1384980.1"
                     /translation="MEFLRRNRVGLVIIAILTCLFLILIPKQLSKKSKIVSQTPFLIE
                     IKLPDPAYSSPISIEEALKKRRSIRQYKNQPITLQQASQLLWAAQGITSDTGLRTAPS
                     AGACYPLELYFIASNVSNLSPGVYHYEPTKHTLKQLKAEDISSQLAQAALGQQAVKSS
                     AANIVITAVFSRMTKKYGKIGRRFALMEAGHVAQNIYLQSVSLNLATVSIGAFDITQV
                     RESLGIKDMEPVYILPVGAK"
     gene            1731..2900
                     /locus_tag="VAH76_000322"
     CDS             1731..2900
                     /locus_tag="VAH76_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384981.1"
                     /translation="MVQRIRRYAKKIYEDKFRFILIILILLLIAPMLLISQPKTVEGK
                     GIEMHFFYTPSCPYCAKQKLFNKRLLKNYKVKIVSHDITDPIEKKLFIFTAKQHGIAQ
                     HNLGVPLTIVNGKTIIGFESQESTGRLIEQALEQGDTKQPVSQIKPVETQKIPILGEV
                     DITHYSLPTLAILLGLIDGFNPCAMWALVFLIGLVTGLHDARKLWLLVGTFVFSSGVL
                     YFLFMTAWLNAFLLIGYIRPVVILIGAFAIILGILQIRDFFTKDIACPIPTQQKQKVM
                     RRMKILVLSPLTITTITGIIILAFVVNLIEFVCSSFIPAIFTQALALHELQTWQYYFY
                     ILLYDIFFMLDDLIIFSFAVFAVNRISTQYARWCKFIGGITLFVLGIVLLTTSNS"
     gene            3155..5044
                     /locus_tag="VAH76_000323"
     CDS             3155..5044
                     /locus_tag="VAH76_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO1384982.1"
                     /translation="MPSTRNIETHNDSNPTLRALSLSALGIVYGDIGTSPLYTFKTVI
                     LLAGGGTPDVTTIMGSASLIIWTLIIIASVKYICFALRIDNDGEGGILALMSLLSLKL
                     KQKPFIIAVGLMGAALIYGDGTITPAISVLSAVEGLEILSPSLKYYVLPIAITILITL
                     FAIQSKGTATIGKAFGPVMAFWFLTIGILGAREVIQHPFVLAAINPVYGLNFLFSNGA
                     TGFFILCGVFLCVTGAEALYADLGHFGTAPIRCAWFGLAFPSLIFNYLGQAALVLEGA
                     STEHNIFYMLCPSDFLLPLIILSTVATIIASQAIITGAFSMTRQAMQLGWLPRLRVTQ
                     TSSEGYGQIYIGVVNWLLMLATLGLIIGFGSSEKLAAAYGIAVSATMLCTSVLLFIAL
                     HKLWKWNIIKSGLVAGLFMIVDASFFAANLTKFINGGYIPITLAIIIYSMMYIWHKGY
                     KTIAIKQKEKNITVDSFLDSIQKEGVVRVPKTAVFLTSKEQDIPPTLVWHVKKNHVLQ
                     DKVIILNINNLSIPWCKPGDQLQIVETGAGIWHAVANYGFMEQPHIPKLLKKLETQGY
                     DINIKDITYYIGHETIFVRNVRHTLSKYIKILFVFMHRNALPMSNYFHLPPESVFEIG
                     RQIEI"
     gene            5262..7012
                     /locus_tag="VAH76_000324"
                     /pseudo
     CDS             5262..7012
                     /locus_tag="VAH76_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7200..8354
                     /gene="nhaA"
                     /locus_tag="VAH76_000325"
     CDS             7200..8354
                     /gene="nhaA"
                     /locus_tag="VAH76_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEO1384983.1"
                     /translation="MNKSDSFYNLETIGGILLFIAAVLAIITANSPYRVGYEYFLSIN
                     GSVSVGNMSITKPLLLWINDGLMAIYFLLIGLEIKREVNRGILSDKTNLLVPALTALA
                     GLLFPALIFIFFNAHHPVYLKGWAIPTATDIAFTLGIVSLLGSRVPFSLKILLTAIAI
                     FDDIAAIVIIALFYTEQLSLLSLSLALVFTLILIGLNYFKCRRISVFMLFGVALWIAV
                     LKSGVHATLAGIVIAMTIPDEGKESMLTRLEDGLHHWVVFLILPLFAFANAGVSFVGL
                     DASMLTHPVVLGIGLGLFLGKQLGIFLSLGYFVQFKKFLKADKVNLAQVYGIALICGV
                     GFTMSLFIGSLAYQNYDLSLMPMVKIGVVFGSFIAGLTGFLVLKMTSLKR"
     gene            8406..9146
                     /gene="phbB"
                     /locus_tag="VAH76_000326"
     CDS             8406..9146
                     /gene="phbB"
                     /locus_tag="VAH76_000326"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEO1384984.1"
                     /translation="MERKIAIVTGGTGGIGTAICQRLAADYSVIACYFKNGNHEEAIQ
                     WQRTQQQAGLDIDILYADIAKFNDCEKLVALVMERYGRIDVLVNNAGITQDSSLKKMS
                     PEQWQFVLDANLTSVFNMTRNILPAMLEKGYGRIISISSINGRKGQFGQCNYAATKAA
                     LFGFTKSLALEVANKGITANTISPGYIETPMLSTMREDVLNTIISAIPVGRLGLPQDV
                     AEAVAFLASPASGFITGANLDVNGGQYM"
     gene            9232..10170
                     /locus_tag="VAH76_000327"
     CDS             9232..10170
                     /locus_tag="VAH76_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO1384985.1"
                     /translation="MHRFHNILFVSHGIKEETEALQLALRLAAESQAQLRVLITCPPF
                     PDTLSEYKISYEEYLIDKMNKTIQSAKSGLGFSKKKIHIPIEIECGSMPDVRIIRHVL
                     KHAHDLLIKEAETPESKKGFKAVDMELLRKCPCALFLYRPLKNTLQDIRVAVAIDPKD
                     EESVAQDLSVRLLDVSHSLNAFYQGNLRIVSCWDFVLENYLRDSVWLKISEAELEKMV
                     VEEKHTHGLLLRELIKRSHISGEYHIEHLKGHPEDAIPHLITHKKIDILVMGTVARTG
                     IAGFIIGNTAENILQKINCSLLALKPQGFVSPVKAY"
     gene            10188..12935
                     /locus_tag="VAH76_000328"
     CDS             10188..12935
                     /locus_tag="VAH76_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO1384986.1"
                     /translation="MVEQQNSSEPDWHEQSVEKTLVMLNSTEQGISDQESKKRLAYYG
                     FNRLPEPKRNSLFLRFLLQFHNILIYVLLGAALVTSFLQHWVDTTVIIAVVLINALIG
                     FIQEGKAENALEAIRNMLSPTATVIRDRERQRVNGEQLVPGDIILLEAGDKVPADVRL
                     IKSHGLKVQEAILTGESIPVEKQISPVASNTVLGDRTCMAFSGTLVTNGQGKGIVVAT
                     GSSTQIGHISGLLSKVESLTTPLIIQMGLFAKWLTLFILVLASLILAYGYFVQHHPFG
                     ELFMVVVGLSVAAIPEGLPAVLSITLAIGVQAMARRHTIVRRLPAIETLGSVSVICTD
                     KTGTLTLNEMTVSSVLTGDHLFILDGVGYEPQGTITLNEQVIDNNKYPLLKKLARAAM
                     LCNDAILHNQEGNWTVEGDPMEGALLTFAGKTGLILQEENDLWVRTDVIPFDARHRFM
                     ATLNHDHLQHAMIFVKGAPEQILKMCQNQQINQGEVQPVNESYWKEQMDQVAARGQRL
                     LAFAVKKTKPEHTILEFTDIETGLTLLGMVGLIDPPRPEAIEAVAQCHTAGIQVKMIT
                     GDHASTALAIGRQIGLKNLDKVLTGLDLDNMDDAILKNAVLETDIFARTSPEHKLRLV
                     MALQSHGMTVAMTGDGVNDAPALKRADAGIAMGRKGSEVAKEASEFVLVDDNFASIVA
                     AVREGRTVYDNLKKVISWTLPTNAGEAMTIILALLLGLSLPVTPIQILWINLITAVTL
                     GIALAFEPTEEGTMHRPPRPRSEPLLTGSLVWHIILVSILFICGVFGLYFYALDRGYS
                     IELARTIALNTLVVLEIFHLFYIRNIYSTSLAWKAVQGTKVVWMAIIVITVAQFTITY
                     FPPLQTIFATESIPFWDGVLIVATGAIFFAVIEIEKQLRLRISAISHNSADAHYGSSV
                     L"
     gene            12932..14641
                     /locus_tag="VAH76_000329"
     CDS             12932..14641
                     /locus_tag="VAH76_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1384987.1"
                     /translation="MMDWSLLHASPFYELTSLLLLATIIGFFCLLLRQPMIVGFIIVG
                     ILVGPAFLGIVRSHQHIELLAELGIAVLLFLVGLKLDLHLIKTLGLVSLATGLGQVLF
                     TSLIGFFIAKGLGMDSVTALYVSVALTFSSTIIIVKLLSDKHEVDSLHGRIAIGFLIV
                     QDLVVVLAMITLSAFGIGTENSGTALTTHITKVFLYGVTLLIFVGLFIRYLANPLINR
                     IAHSPELLIIFAIAWAAFMATMGSYFGFSKELGGLIAGISLASTPIRDTIAARLSSLR
                     DFLLLFFFITLGSQLSLNQLGHQVTPAIVFSLFVLIGNPLIVLAIMGAMGYRKRTGFL
                     AGLTVAQISEFSLVFMAMGVTLGHVSTDSLGLVTLVGLITIALSVYMITYSQTLYHWL
                     EPILSFFERGNPYREKIEIHPSMNKTDYDIVIFGLGRFGKAIAKLLIERKLNVLAVDF
                     NPDEVREWEYQGYNATYGDACDIEFVTSLPLNQVKWVICTIPQHLLGVTYQDPRVILT
                     ETLKQTKFSNRIAVTTHHTSEVESLKALGVDVILQPFYDAAARAVEIILDVSENNSIK
                     TVN"
     gene            complement(14665..15555)
                     /locus_tag="VAH76_000330"
     CDS             complement(14665..15555)
                     /locus_tag="VAH76_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO1384988.1"
                     /translation="MTLLNNLLIASDFSKHADWALQRAIGLAKLNNAHIHFLHVVTPP
                     LSSIAQSSETELKPNHLSEKKAIEEKILKQLGENNRKLFANTSVVLGRASDEVVRYAD
                     ENHCELIIVGAHGTYYINDYVLGTTSGSIIRQSHVPVLLIKAEPDFSYNKILIATDLS
                     EASKETLQFVFHCFPNATFQLLHIVDVYYRQFLTPNDLDEKCTDTKHPKIKNILEKLD
                     VFLSKCEVDKSKFEKKIIGGYYADAIIEQANHWKADLLAFGTQGKSGLHYLLMGSVAK
                     RILYLSSVDMLVVPPKLKSS"
     gene            15826..16389
                     /locus_tag="VAH76_000331"
     CDS             15826..16389
                     /locus_tag="VAH76_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384989.1"
                     /translation="MTIYFLFGKTGSGKSYIGELLRQLNIVHIDGDNHITQKMRDCLI
                     EDEQMTPEMIDEFVDVLIDVINTQKEKTPKESFAISQAMYLDKHRIKLLNAIPELKFV
                     MIDVTPVLRASRITNRFRNQESKVSLRYATKMDTFFEYPSHETIRLENNQGSDETLIE
                     QIREKMPALFNMDSKEELNPGIKLQFA"
     gene            complement(16504..17430)
                     /gene="leo"
                     /locus_tag="VAH76_000332"
     CDS             complement(16504..17430)
                     /gene="leo"
                     /locus_tag="VAH76_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease Leo"
                     /protein_id="HEO1384990.1"
                     /translation="MKNKIVLLAPVSLSFLFSCSTYAADYEFNGLSLSASLGILSGKA
                     HEYVYHPETDTKLSQLDWRIKNAPIIKGELNYDFLDWLSMNSRGWTTLVKNKAAMDDY
                     DWLNPYQDTWTHWSHHEDTYLNYANELDMSLRAWLMQKQNYKLGLATGYQWNSFDWKA
                     TGGCYQYYGGLYAGCFPGDQPGIGYQQKFRTPYIGLAGKYFINNFEFSGLLKFSDWVS
                     ARDHDEHYARNLTFNERGNSSRYYAATIHSGYFLKPNTKIFVEASYNHYSNGRADTEI
                     IDNDTGERSYFGDSAGLSNKHYTVALGLQYLF"
     gene            complement(17558..19165)
                     /locus_tag="VAH76_000333"
     CDS             complement(17558..19165)
                     /locus_tag="VAH76_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO1384991.1"
                     /translation="MNKNLSALSLLWISITSMVGSGWLFGSLYSAHFAGPAAIIAWPL
                     AGFLLLFVALSYAELGTMFPQSDSLACLPLYTHGRLTGIIMNVMTWFSLAILPVIETQ
                     GVIQYASNYIPDLVSHDALHYRNTPAGYMLSLVVLMSFVLFNYFGIGLFARINAAFTI
                     WKIIIPILTIASLVTMSYHPGNFFQHGGFMPYGWQGVMAAMSSGGVLFSLLGFRQVVI
                     MMGELEKPGQYIPLILGGSLVFVTLLYTGLQWSFVGSVSEQALEKGWVNLSFTGDAGP
                     FAALAALAGMLWLSILLYADAFISPYSTGLVYSTTAAHMLSSMGSVVPAPVILTKQNR
                     YQVPWVSLGVNFLLAATMSLVLHGWQEMSAFLVAILMISYSIGPVALVCLRQQLPHYK
                     RPFRLQGSNLIGFLGFYVCTVGVYWAGFHSVRKLLVITILGVVCSFFYCLIKNTHREI
                     DSKQALWFIFYLFGLGIFSFLGNYGGKHILPQYWDLLYLLVFSLLVFGFALLSRKPSA
                     YTQNMVAQNTNTKITAPAFSIERYDIA"
     gene            19495..21660
                     /gene="katG"
                     /locus_tag="VAH76_000334"
     CDS             19495..21660
                     /gene="katG"
                     /locus_tag="VAH76_000334"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEO1384992.1"
                     /translation="MDGKVGNTTTGCPVMHGGMTSAGTSNTAWWPNALNLDILHQHDT
                     KTNPMGKDFNYREEVKKLNFEALKKDLHALMTDSQAWWPADWGHYGGLMIRMSWHAAG
                     SYRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKISWADLIVLAG
                     TIAYESMGLKTFGFGFGREDIWHPEKDVYWGSEQEWLGAKRYDDKDRQSLETPLAAVQ
                     MGLIYVNPEGVNGQPDPLRTAQDVRVTFGRMAMNDEETVALTAGGHTVGKCHGNGNAK
                     FLGPEPEAADIEDQGLGWINKTTRGIGRNTVSSGIEGAWTTHPTQWDNGYFYLLLNYD
                     WELKKSPAGAWQWEPIHIKEEDKPVDVEDPAIRHNPIMTDADMAIKMDPVYRKIAERF
                     YKDPDYFAEVFARAWFKLTHRDMGPKTRYIGPDVPKEDLIWQDPVPAGNRAYDIAAAK
                     AKIAASNLTIGEMVSTAWDSARTFRGSDKRGGANGARIRLKPQKDWEGNEPQRLTKVL
                     RILEDIAADTGASVADVIVLAGNVGIEKAAKAAGFDIIVPFAPGRGDATDDMTDAESF
                     DVLEPLHDGYRNWLKKAYDVRPEELMLDRTQLMGLTAHEMTVLVGGLRVLGTNHNNTQ
                     HGVFTDRVGALTNDFFVNLTDMANVWIPSKDNLYEIRDRKAGNIKWTATRVDLVFGSN
                     SILRSYAEVYAQDDNKGKFIQDFVAAWTKVMNADRFDLA"
     gene            complement(21971..23248)
                     /locus_tag="VAH76_000335"
     CDS             complement(21971..23248)
                     /locus_tag="VAH76_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384993.1"
                     /translation="MKINKTTNDQNADQKSSQYIYVEKKEIGGIDTEIITESYYPIPW
                     GSASERPLDYYEKQSKIITYYKEDIDLDGNPFNKKIEIEIPKVDEGENRHRRIKVKVD
                     GNPIIFQDNNILDMDETFNIRDVNLLIHMVLNNFHPKSNSTCLQTITPFIHLPGTGGD
                     EDYEFNQKNNGDVSKVLLPYVQEMCSLNNSSVVRQLLIPVATKGGLHHWNLCMLFIDE
                     NNIPSLYYIESENLLASFDYYTYMYYMEDYKMHILPQVNALLAKFHYPAIDKINFCQA
                     KQFSQEGCGIAMSLNMHKILEEKYPQVICYSNTPRKGIPSHTEIRCLSSNVSIEEDAL
                     MRVELAFKIQARASIIAEGALDAKMVEKKSLVCSEILSDSRSLSVKAKEAFGFFQSAQ
                     PGISSEMHSVMAEDKGNDETSSSDINAEACRLW"
     gene            complement(23807..25039)
                     /locus_tag="VAH76_000336"
     CDS             complement(23807..25039)
                     /locus_tag="VAH76_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1384994.1"
                     /translation="MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTAL
                     IATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPTYQEAG
                     ILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNTRHKGLAVSFVTLGSICGLLLAII
                     VSKITLIFFSSQSPYFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPIL
                     NAWRYRKKAIAAIFLLAIYCNSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMTCYSM
                     GLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLQITVACSVLAVIISFF
                     IGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMV
                     MALLTLPALWLIQEKGTN"
     gene            complement(25080..26384)
                     /gene="sdbC"
                     /locus_tag="VAH76_000337"
     CDS             complement(25080..26384)
                     /gene="sdbC"
                     /locus_tag="VAH76_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbC"
                     /protein_id="HEO1384995.1"
                     /translation="MPNSSVQIWLQRQIALRTFPPNREDWFVKNGFGHSEMSLEERFA
                     CFVEAIRQVDSPYHEQLSGIDFQRFEIEVTLANQKTCMTEVIGFQNNSNQSSSLLNEK
                     VKAVGKGKHVIYFTGIGTQYQDCLIDIAKAVQITGAHYYAFEYPGMRKLGGEVLEVND
                     MVNTGIALANTLLEKGISIDDILFQGDSFGAAVAKKVSDQFKLQSNVTVRCILNNTFS
                     TFQAAVEGNLGAFSTLRYTVRPLLRYTGWDIRPGDNYGQDTPYQIHINHTGDLTLGAG
                     HATLAESVESNSQLEHFVDPCPEEYRIKRDIYKQWHWASLSEQGITFLETKYGRNSDG
                     QLDTHLADLYLLKYDTGLGVYESFICKYLEDSNDYIANHPQKLSLDELPSPLESETVS
                     LRQLIVSVLPSRPSIAGFFGRPIPVKDEVDSENQAQKSIELK"
     gene            26645..27529
                     /locus_tag="VAH76_000338"
     CDS             26645..27529
                     /locus_tag="VAH76_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384996.1"
                     /translation="MFYELILSKSSNLIQEFSYIPHGVTSLDLSLNELGSISNAELIQ
                     AFKYIPESVTSLDLSNNHLCDKSGAELAQLLAAISANVTSLNLSSNYLDRKSGAELAK
                     AFAAIPSSVTSLDLHCNSLGNNRGVELAKAFASIPASVTSLDLSMNYFDLESSADLSQ
                     IFTSIPPHVVSLNLSFNSLHEVPFEKLVLLKDSLKHVQTVYLSFYSVKEMSKEQRSSL
                     GSAFPNAQKIILVDDYDNEIQPSITISNLIGELSGKADAPSLLNQCILFAQRNQIDYM
                     KRNIPGELQESIRAFNSR"
     gene            complement(27650..28114)
                     /locus_tag="VAH76_000339"
     CDS             complement(27650..28114)
                     /locus_tag="VAH76_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEO1384997.1"
                     /translation="MKHRRSETQDIDDAIIKTLNKLLEIELAGVVRYTHYSFMVFGFN
                     RIPICKWMRDQAQESLNHAHLIGELITHFSYHPTLKIGKLLETHEHNISNILEESLEH
                     ERQSLDLYYQLLQYSENKSVLVEEFARQMIQEEEMHLGEISKMLNSPTTFKD"
     gene            28513..28872
                     /locus_tag="VAH76_000340"
     CDS             28513..28872
                     /locus_tag="VAH76_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1384998.1"
                     /translation="MSNKAKNTHLKNGDSQTKQAYTNQASPLFFSQKQITPSSGTLET
                     KKPQEGKQPTQEISLETTTDILLAAILQELKTQNMIELMKLKAQQEQEQEELQAIETM
                     KEKDDARFNEIRQTMYA"
     gene            complement(28999..30069)
                     /locus_tag="VAH76_000341"
     CDS             complement(28999..30069)
                     /locus_tag="VAH76_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1384999.1"
                     /translation="MKTGRFDQAKAEAFAERLLSVLNDAALTLMISIGHRTGLFDVMD
                     ELDFASSEVIAEAAGCNERYVREWLNALVVGGIVNYDAHAKTYQLPAEHASFLTRKSS
                     PQNIAVIAQFIPVLAYVENGVVDAFKHGGGVPYEAYHRFHEVMAEESGQTILSSLADK
                     IFPMVHGLVDKLNQGIHVLDIGCGYGRALILMAKTYPQSQFTGYDLCDETIAHAVHAA
                     KENKIQNIHFEKMDLSVWDEQEKYDLITSFDVIHDQAKPAQVLSNVFKALKPQGIFLM
                     QDIKTSSDVAGNVHHPLGAFIYTISCMHCMTVSLSQNGLGLGAAWGEELAEKMLVEAG
                     FGKIEKHYLEHDIMNTYYVMRK"
     gene            complement(30115..30675)
                     /locus_tag="VAH76_000342"
     CDS             complement(30115..30675)
                     /locus_tag="VAH76_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO1385000.1"
                     /translation="MDDNYKVSSMINGVDIATLKKTIGTIQKDPKLGQCEFRLKNEWI
                     NGDSNRSYVDTFYAASSEQHHKKQFHFQAGEPPLLAGHDEGANPVEFLLSALSGCMTT
                     TIVYYAALNGHVIEEMESSYKGDLDLRGIFNLDQSIRPGYQKIHIHFKIKTDATPEEI
                     KKYYPFSPVYDVVSNSVPVEVTIEIY"
     gene            complement(30851..32050)
                     /locus_tag="VAH76_000343"
     CDS             complement(30851..32050)
                     /locus_tag="VAH76_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEO1385001.1"
                     /translation="MIKSFRVTIFLFLVSGLILARDMHAFPSGLESNQDKLVYFQLEA
                     ETFYSPKPVTIEGQFYLTYEVFLANVGDYLFSLENVEVLNGRKPYKVLFSYNQEELRK
                     MIYSFSGHSNHLTSDLTLRPGERGLLFFMVKFDSLDEIPEKITHRFHLYSSKTKISEE
                     GVRYSFLAAPAYVKITKPLVIANPLDGTHWMAINGPSNHSIHRRARVAAHGIVYYPER
                     YAIDFMQFGVDGYVYKENPNKNENYYSYGANVYSVADGEVVAVYDGVPDNTPPGRDYP
                     VTLSNMAGNHVIIKIGKHQYALYAHLIPHSIKVTKGELVRKNQLIAKLGNSGNSDAPH
                     LHFHVIDKPSPLIGQGIPYAFEHFSTEEYRFAGDEWEFPIVYEGRLTEHHHELVHENA
                     LMNFEEK"
     gene            complement(32105..32677)
                     /locus_tag="VAH76_000344"
     CDS             complement(32105..32677)
                     /locus_tag="VAH76_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385002.1"
                     /translation="MALIRDSKSQVMSVFKFQPLGIKDLNQLYQWFQGPIINHWYARD
                     KSWSLDEITEKYEPRIAGMEHVPSFIVYKDDLPFGFVQFYLLTDSLPDGIDGYESLLF
                     EKYNPQDLAGIDLFIAESEGRGRGLGVELINRFIDKFLTRFKAVVVDPDVNNQQAIRC
                     YEKAGFQQTDFSQDPNHLIMIKPLSLITGN"
     gene            complement(32867..34213)
                     /locus_tag="VAH76_000345"
     CDS             complement(32867..34213)
                     /locus_tag="VAH76_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEO1385003.1"
                     /translation="MRKWIISLLLLIIIVLCSYPIIGMMALSTVKKNVDNIPKSPFLT
                     WQLKDNQSGWFCSTAVLNLLVDLPAQENKDAQGNIKIQPALHLSMDFPMIVRHGPFIM
                     TNNGLRFGLAWVTTKPETHYGALINYLNQTAVSYSLPSINMKGTNNGDQGKFVFVWQG
                     LSASLKVNPRIDKASGHVTMYGMSTQVQETDVRIGKVKVNFALNRYMDDLWLGDSSIM
                     LSSISTVTNSQPVFDLQNLGMDAWAGVNNNLLDFNLQLSLNKFVMNNNSYGPGQLKIE
                     VKNLDPQAMAKLNHLNYDYPSNQIAILAEIFNLLSKGATVNLNQELDTPQGHLSSSLN
                     LALTKSSFSNPEDLLQNLKGNGQFKAPIKLVEQLLFESIKSKTATPAADMSQSQATEP
                     LIDVDAEAHKQTEELLQKLVSKGILKVEGDYYQASFTLENTKFTVNGQPFDPALLN"
     gene            34683..36197
                     /locus_tag="VAH76_000346"
     CDS             34683..36197
                     /locus_tag="VAH76_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO1385004.1"
                     /translation="MSYMKIEELLLKDTMRRAQIPGVSIAYVNHQGIISTQEIGLTDG
                     CGLVKMSNDPTQCPFQELVLGSKSELGVGTKNTVITFNKELYYVDQAEKVVWKIPLTE
                     ANKIVYEMLHAKCTETYQLADSNEHEFIAALTGRMPPTEVKPETVFGAASLSKPVFAY
                     LVQKLIQTNAKNQSEAGFDQFILPEKLKHFDLDTPLFHIIPLEEFNIDGMKFDLSDES
                     IVAYSKILTARMVLSHTTGLAHGEMKFQFLPDSEEKEHGYSNVGIIYLQQVIEKLTGS
                     DLETLTQKHVFEPCGMIHSTYGLKACAANSLRTTACDYANFVKHLIHDSTIENPFVPH
                     AYMTKDKGQAGAIGITKGNIPDAILQHVAWGLGWGLQTNDEGRVITAYHSGDMNDYRA
                     WVVIDLQDKDKKNAVVFFANSHNGHILAEQIIPKTMQMEQAANYFFSKWGFARNLAEL
                     GGKTTRLGIKSSSGSSDSSPDTSIQDEPVEIKKETEKSDTSSIETYTSGWTFNA"
     gene            complement(36174..37094)
                     /locus_tag="VAH76_000347"
     CDS             complement(36174..37094)
                     /locus_tag="VAH76_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1385005.1"
                     /translation="MAIPFKRINLNLILHLDALLSEKSVSAAAEKVFVSQPAMSSSLK
                     QLRELFKDPLLIPGKGEYFLSPKAKELQPQLEQVMFFIRNILQPNERFDPFQTKRSFK
                     IALPDYVELILLPSLMAALEKLPHIVIETTIQSMADDPHLFIDENVDLAIGAINENSL
                     SAQLKVEKLYEEEIICFASKNNPLINRPLSLEDYISNKHIAFSFNQERYSHSAEYLYK
                     KKLVRNDMLFVKNILPAIFAVANSDAVISTAPSFLINMFARQFNLGMQALPFNLEPTP
                     IYLVTHSKSQYDEGVSWLAKLISSSIKSPS"
     gene            complement(37278..38792)
                     /locus_tag="VAH76_000348"
     CDS             complement(37278..38792)
                     /locus_tag="VAH76_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEO1385006.1"
                     /translation="MFGELFASSIVLGVRYYSNQAYQAVAGLLGQIERFGIGYTREFI
                     RDPKSMIQSLTSKARSSEAGYSVVSLGYLGGNYAVITPKSQSQLTELLNQFDQEKSGR
                     RGHASFKFFSGNDFFIISDTGHAALSSRKNLSRFLVPSRLVNPTITEVEKRVDCDADI
                     QLRHTICGIVRAVMVENMLGIKQLPDNTYDVMASYRNDVKRWGAFPFPELLNFMPSLR
                     QKRDVYRAFSKGILEQEFEKLVEVLHDDPNPGNTNLIAASVVSLFKDENPTLSNEELL
                     SALRSLSIEDIRKYFENPIVQSLPMILKAADNLTDAIVLCLEQIASDPDKFAMLRDEM
                     DRSHLGIEEGIDVRALKTLPVLDAFYKESVRFDAPVAVPRYTQNGYSSDSMTIPPNTM
                     MIFDLHALAKGEQYWTNPEEFDPTRFLQQEKEQVTEEGGMNSNNKNHTVGQFPFIPFS
                     VGLRNCPAFAVTESLFKVTIAKFVSDYQLQLVKKDEEDSTLHITSRDKSFGLMQ"
     gene            39089..40003
                     /locus_tag="VAH76_000349"
     CDS             39089..40003
                     /locus_tag="VAH76_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385007.1"
                     /translation="MATTLIQTPSYTKNLTLNLDDYPGGVAIWGALPALFDTSNQGFD
                     RGVHVHARLADSSKKVIDATYDHVTIISGYRIFTITEEAAVHFSMSAIFDIKITSLTC
                     QHCSQLITSVGYAAVRPSRQHQCNHCGEITTTTSDCISNPIMLLKELIGDEQVKRPAV
                     IPNRTIAIDPDKYSGGIQIWGSNPSIIWTAKRLEESAIHIHAYNENGKRIIDNTYGSV
                     SLDGHKLDIEMIRVLQIQLALPNLALLLTTVYCPHCGVEQFDRGIWAVSAHNHRVCLL
                     CKQTFISQDVISNPAFDVLTHVSGVISQ"
     gene            40000..40401
                     /locus_tag="VAH76_000350"
     CDS             40000..40401
                     /locus_tag="VAH76_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO1385008.1"
                     /translation="MSTLKTIFKIGALIFNEKNQLLAVHKKGKPPMELIVPGGVMEEN
                     ESDEETLRREIKEELDSDIISFQFYKQFEDKAIYEEKWLVMRTYIVTLGNAPKPANEI
                     DQLVWLGHDYHQSGYRFGSILGKQIIPDFFK"
     gene            40515..41612
                     /gene="lem23"
                     /locus_tag="VAH76_000351"
     CDS             40515..41612
                     /gene="lem23"
                     /locus_tag="VAH76_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem23"
                     /protein_id="HEO1385009.1"
                     /translation="MKRKDEQNNLTKGKANEQNTVSTNTHLLFSKPPGINADTANHMG
                     SSIDRLKDFDSSKQSLWLKYSEEIINEFCALLPEFPKKKPKVREDIRCPEEALLTQAG
                     CAYAFLASKRREVAKLLKHGPLRHQFGLFNKPGFASIDDFNLSKDIPSQKDKIYEHLS
                     LLISQPNPFIEVKKNHREAEGFSIYTITFKMKPDEIEQVHKKNLDSNKPFIENDMVLT
                     ITFNDYGKNDNRTSIVIDHCPTPFAENYTSESSYFKVQFELIEPYFQACQAWEASDGI
                     DQFLKNAGRIAYILARMQPVGRGNSAIVEWMIRGLAQIKSIELGPFNPDEKIGWDFKA
                     FLTPEIQDYAHWFSEKAFAYSQLKNVTSLGH"
     gene            complement(41794..42099)
                     /locus_tag="VAH76_000352"
     CDS             complement(41794..42099)
                     /locus_tag="VAH76_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2407 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385010.1"
                     /translation="MYNVVSFNNEGVEHMKPTSEKSKEIRKKYKAPGASDEQLANSIK
                     VASEFEYKNSSTFGWGGVYMVDRFQSMITGRAITNNTQRLLDAQALEEEASKANLKQ"
     gene            42322..42981
                     /locus_tag="VAH76_000353"
     CDS             42322..42981
                     /locus_tag="VAH76_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385011.1"
                     /translation="MLTKNLNEALSAIKYVKSKLVYGSNAEEDSKKPNWEPGRIILLR
                     KEVHALAVVYARSHNGKAGFDVISKLTHAALLKYGFGNCGEQAQTAFCYLQSRGVAPL
                     DYCQTSIGEHCLIVIGRVSGSDPNDISTWGEDAVICDPWAEKAYPLSEFRAMQSPDND
                     IRYPEICYANGKSPKPYLAGELISHHRIEPASKASSLSFFKKENYEHSQLNSATPMLI
                     A"
     gene            43306..44124
                     /gene="ceg29"
                     /locus_tag="VAH76_000354"
     CDS             43306..44124
                     /gene="ceg29"
                     /locus_tag="VAH76_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector Ceg29"
                     /protein_id="HEO1385012.1"
                     /translation="MTNSNHLRNPVTKSLNDLCQDELSVIGSYLGAWNDLWSLTFTSS
                     EMKKCLIHQQTDELWKFFFKEFFPYIPLPTEDFYKNFMKNYKTWYQTLYRVKQDPFFL
                     MYLDKLKEDPAHLFRNDKEIIIAAIEKQPFLYKNFSRGYKEDEEVILCALKNNRFIIH
                     DVPRTVREGFKEIADIKEDKARVDACDKLIGKLSPQRVSKIKSLDLFKSQLTLQAQNI
                     TKKSGEQVNDRQLQRSTAPITPEGITSLVHLGLFKDTQIQYEEKEESLHNKYRK"
     gene            complement(44245..45942)
                     /locus_tag="VAH76_000355"
     CDS             complement(44245..45942)
                     /locus_tag="VAH76_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1385013.1"
                     /translation="MIAKSKTDMNRIQMGTNKEFPSKSHLKPSKSCGTDGSNFQVEFL
                     LDVIQKLSLAKDLETIMFIVRKAARTLTGADGSTFVLRDGNQCYYAEEDAIAPLWKGK
                     RFPMSACISGWVMEHRQSVIIEDIYSDPRIPIDAYRPTFVKSLAMVPIRTAAPLGAIG
                     IYWAEYYFPTEEQLNWLQVLADSTSVAMENVMLQSELEKGQKETTAQIEMTRKLMEVN
                     KNLECALYELNHRNKEMQWLKELSSTLQTCLYIKEAYHLIALYAAKLLPESSGIFYVM
                     HPSRNYLEAMSSWGDSLIEERIIKPDDCLGLRRGSIYTVEEPQRDLICPHHKINSNLK
                     PYTCIPLFAQSDILGLVCMEWKSFKKEAEEQINKENNQEILASMMAEQIALGISNIML
                     RETLRNQSLRDVLSGLYNRRYLNEVLDRELNRCARKSLSLAVLMLDIDHFKQFNDKFG
                     HEAGDMVIQAFANVLRQVTRKEDIACRYGGEEFILIIPEIDLEAALQRAQSIHDAVSR
                     IHLRYGGNALTQITISIGLTMYPAHGNDMQDLITAADNALYEAKNSGRNKTVLYHKN"
     gene            46301..>48056
                     /gene="vpdA"
                     /locus_tag="VAH76_000356"
     CDS             46301..>48056
                     /gene="vpdA"
                     /locus_tag="VAH76_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VdpA"
                     /protein_id="HEO1385014.1"
                     /translation="MKTKQEVSQQDKLKDSKSSTPLQTKETWFISDTLNITFDSYDFS
                     ISVTEQAPMPYRIVFSGGGSRILAHIGAMDELIRHGLQFTEFSGSSAGAMVAAFAYLG
                     YNCSEIKQIISWFNEDKLLDSPLIFNFNNIKQIFNKGGLSSAKLMRQAANYVILKKVM
                     DIISDEKFKTRFAQFQNFLEENIYSCPENITFQTLARIKEICPECELGEKLFITGTNL
                     STQKHEVFSIDTTPSMALADAIIISANLPIAFERICYQGNVYSDGGISNNLPAHCFSE
                     KGHKTTFLKHKDDVDFSVLALQFDNGLEENALYSQNPIPKWSWLSNTFYSLITGHPNV
                     TENWHEDLQILRRHAHQSILIKTPTIALTNLTISQDTKKALVESGRTAAKNYLDLHEF
                     CMDDYGNIRHNECLHEKFQKPEELLDYCVLHSHFELLKKIKQAISCSQYLEKGYKHYL
                     CELCDNLLPPQLKCPNEGSGTEQPEIKLEKDTIICEKNNNSGLTFSMTFFGVPSPLVK
                     TLNKDSPELKIKLFTGLYPILIQNWQNLCPISGISGILNSIRMSFVEISSTDTCIKTL
                     IDKLNEIEIGHFLIFVFKA"
BASE COUNT        14994 a         9569 c         9131 g        14362 t
ORIGIN      
        1 tttttaacta aagataagtc actttttgaa tagtcatata tgctgcaagt agtggagtca
       61 aaaactgcca gccatgttgc ttgttcggat ttcatattac ctccatgccc ggaatgagaa
      121 ttccatctat gaactttaat aacatcaaaa gtcaaacagc tatcacctat caagatgacc
      181 aagcctatat ttttgtgtca atatttgact tgggctttat aaaattgcct cgtagtatgc
      241 ttctgcctga taaatattac cttggagaca aattatcaac ttatttgaat cagtccaaaa
      301 tagttggatc tcatgcaaat acagcaagcc atatccatac ttttttcaag gcgtacaaat
      361 tatttgtttt ttaagtatag cattttcttt gttcaacaat tatttttgta tttataaatg
      421 cagatcaccg caaatcaatg aagcttagca cccatgccag tttatggagc aactcggata
      481 ttccaattca tctcaacgat aagataactg gtatgccatt atggattggt ctttcaaacc
      541 aatagctaat ccgatttaga gtaatttcga ctctaccaat ctcttcgatt tttcagagac
      601 aaagtttccc ataactcttc tctgcaaacg gtttgattgt taaatcatta gaaaatctta
      661 cctttataat ttatattttt cattttgctc ctaccggcag aatatataca ggctccatgt
      721 ctttaatacc cagagactcc ctgacttgag taatatcgaa cgcacctatt gatacagtag
      781 ccaggttgag agacaccgat tgaaggtaaa tattttgagc aacatgccct gcctccatta
      841 aagcaaaacg cctccctatt ttaccgtatt ttttggtcat tcgagaaaaa acggctgtga
      901 tcacaatatt tgcagcgctc gattttactg cctgctgccc taatgcggct tgagctaatt
      961 gtgaagagat atcctctgcc tttaattgtt ttagtgtatg ctttgttggt tcataatgat
     1021 aaacaccagg tgataaattg ctaacattac ttgcaataaa ataaagctcc aatggatagc
     1081 acgctcctgc tgaaggtgct gttcgcaaac ccgtatcaga ggttattcct tgcgctgccc
     1141 aaagtagctg agaagcttgc tgaagagtta tgggctgatt tttatactgc ctgatagagc
     1201 ggcgtttttt aagcgcttct tcaattgaaa tggggctcga gtaagctgga tctggtaatt
     1261 ttatttcaat cagaaacgga gtttgtgaga caattttaga tttttttgac aattgttttg
     1321 gaattaaaat caaaaaaaga caggtcaaga tcgctattat tacgagcccc actctgttgc
     1381 ggcgtaaaaa ttccatttac tatccccctg gtaataactc ccaaaataag atatttattt
     1441 tttgtaccgt ttttctcctg gattttgata ttcaatagaa ccctttattc tgtatgctgt
     1501 caatgttgat ttaaaaatga acttttgggg aaatcgcttc gttacccaaa tttttaactg
     1561 cctgattttg ttgcttggga ttttcttttt gataacaata gaatgtgaaa ttttaccaaa
     1621 ctcacttgat ccgtgaaggt gtgacgtaag gaatgcaggg agtttttgct caattctcat
     1681 aaaagatatt gattggcatc aaaactatct ttaagaatta aaggaacata atggttcaaa
     1741 gaattcggcg ctatgcaaaa aaaatctatg aggataagtt tcgatttatt ctaataattc
     1801 tcattctatt gctcatagct ccgatgctat tgatatccca gcctaaaaca gtggaaggca
     1861 aaggcataga aatgcatttt ttctacacac caagctgtcc atactgtgcc aagcaaaaac
     1921 tatttaataa aagattgcta aaaaattata aggtgaagat cgtcagccat gacataacgg
     1981 accctatcga aaagaaatta tttatcttta ccgcgaaaca acacggtatc gcgcagcata
     2041 atttgggtgt tcctttaacc attgttaacg gcaaaaccat tattggtttt gaatcacaag
     2101 aaagcactgg gcgtctaatt gaacaggctt tggaacaagg tgataccaaa caacccgtct
     2161 ctcagataaa acctgtcgaa acacagaaaa taccaattct gggagaagtt gatataacgc
     2221 attattcttt acccacactc gcaatacttc ttggtcttat agatggtttt aatccatgtg
     2281 cgatgtgggc attagttttc ctgataggtt tggtaacagg gttacacgat gctagaaaac
     2341 tatggctatt agttggaaca tttgtgtttt cctcaggggt tttatatttt ttattcatga
     2401 ctgcctggtt aaatgcattt ctgctcattg ggtatatacg ccccgttgtc attttaattg
     2461 gtgcatttgc aatcattctc ggaattttgc agataaggga tttttttacc aaagacatcg
     2521 cttgccctat tccaacccaa caaaaacaaa aagtcatgcg acgcatgaaa attttagtgc
     2581 tctctccact tacaataaca acaattaccg gcattatcat tttggctttt gttgtaaatt
     2641 taatagaatt tgtttgctca tcatttattc ctgcaatttt tactcaagca ctggctttac
     2701 atgaactgca aacttggcaa tattattttt atattttgct gtatgatata ttttttatgc
     2761 ttgatgattt gattatcttc agttttgcag tttttgctgt caacagaata agtactcagt
     2821 atgcaagatg gtgtaagttc atcgggggga tcacgttatt tgttttaggt attgttttac
     2881 tgacaacttc taactcgtaa cctaattatc aaaggatttt gcttatattt gttaagttat
     2941 ctatgaataa ctcaattcaa acaggattta ttcctataat tcttttaata gaatatccag
     3001 gcaaattcag gttacaatct tttgtcaata atttaatcta ttttctattg caaattcaaa
     3061 gaattagatt aatttatcta agattgaagt gccacttatt ggaggatagc ccctcctttt
     3121 gagtaaagcc aaacttaatg agagagccaa ctcaatgccc tcaacaagaa acattgaaac
     3181 gcataacgac tccaatccca cattgcgagc actgagtcta tctgccttgg gcatagttta
     3241 cggagatatt ggaacaagcc ctctttatac ttttaaaacg gttatattgc tggcgggagg
     3301 agggactcca gacgtaacta ccattatggg ttcggcatca ttgattatat ggactctgat
     3361 aatcatcgcg tctgttaaat atatttgctt tgctttacgg attgacaacg atggagaggg
     3421 aggtattctc gcgttgatgt ctcttttaag tttaaaacta aaacaaaagc ctttcattat
     3481 cgcggttgga cttatggggg ctgctcttat ctatggagat ggcacaatta ccccagccat
     3541 atcggtgctc tctgctgtag agggacttga aatcctttcc ccatcactta agtattatgt
     3601 tttgcctatt gcgataacaa ttctgataac actttttgcg attcaatcta aaggtaccgc
     3661 aactattgga aaggcctttg gcccagtgat ggcattttgg tttttaacca ttggtatttt
     3721 aggggcaaga gaagttatcc aacacccttt tgtcttagcg gcgataaacc ctgtttatgg
     3781 cttaaatttt ttattttcaa atggtgcaac aggatttttt attttatgtg gggtgtttct
     3841 ttgtgtcact ggtgcagaag ctttatacgc cgatctggga cattttggta ccgccccaat
     3901 tcgctgcgcc tggtttggtc tggcttttcc aagccttatc tttaactatc tgggacaagc
     3961 cgctttggtt ttggaaggag catctacaga gcataatatt ttttacatgc tgtgcccaag
     4021 tgatttttta ttaccgttaa taattctgtc taccgtcgca acaattattg ccagccaagc
     4081 cattattacc ggggcatttt ccatgacaag acaagcaatg caactgggct ggctgccaag
     4141 gttacgagtc acgcaaacgt cttcagaagg atatgggcaa atttatatag gagttgttaa
     4201 ctggctttta atgctggcta cccttgggct tattattgga tttgggagtt ctgaaaaatt
     4261 ggctgctgcc tatggcattg ctgtttcagc caccatgctt tgcacctctg tgcttttatt
     4321 tattgcgctg cataaactat ggaagtggaa tattatcaaa tcagggcttg ttgctggatt
     4381 attcatgatt gtcgatgcct ctttttttgc ggccaatctc acgaaattta tcaatggcgg
     4441 atatatacca atcacattgg caatcataat ctattccatg atgtatattt ggcataaagg
     4501 ctataaaacc atcgcgatca agcaaaaaga aaaaaatata acagtcgatt cctttctgga
     4561 cagtattcaa aaagaggggg tagtccgtgt gcctaaaact gctgtttttc ttacatccaa
     4621 ggagcaagat attcctccaa ccctggtttg gcatgtaaag aaaaatcatg tgttgcagga
     4681 taaggttatt attttaaata ttaacaactt gtccattccc tggtgtaaac ctggtgatca
     4741 attacaaatt gtagaaacag gagcaggcat ctggcatgcc gtagcaaatt atggctttat
     4801 ggaacaacct catattccaa aattattaaa gaaattagaa actcaaggct atgacatcaa
     4861 tatcaaagac ataacctatt atatcggtca tgaaacaata tttgtccgca atgtccgtca
     4921 tactctgtca aaatacatta aaattctctt cgtttttatg catcgcaatg cccttcccat
     4981 gagtaactac tttcatcttc cgccggaatc agtatttgaa ataggacgac agatagaaat
     5041 ttagtgaaaa taattgctcc ctgacatgga aaatctcagt gggcgagcac agttttatgg
     5101 tcaggattga attttgcacc tggtcctata gcacatgtga caaatcaaga ttaaatcctg
     5161 gcttttgctc ttttgcagat tatacccagg tcttgaaaaa gattaaaaac ataccaaaaa
     5221 agtggcttat aattataaaa caaatacttt cgatgataga aatggttcaa tttgctaaac
     5281 cacctaatgg ccctagaaat gattatggac gaatcaatat cttaattgag catgagttag
     5341 ggcaatatca aaatatcatt caaaaatgga ttgctaatct cgaagacaac gcgaaacata
     5401 gcccttctaa tatttctcaa tttaaatttg gaaaaatttt aggatatctt ttacttcaaa
     5461 gcttaaagaa agcaaattta cacccagaca acttcagatc ttttggttca gacccttggg
     5521 aagaacctca ttatcaatcg gcacgaatcg ctggaaaata tgtggtaact caagatacgt
     5581 accgaattta cttttctata ccaggagccg atcgtgcgga caaggaaaaa ataaatgccc
     5641 ataaggagca ggcacaaata catcatttca cgactatgac taatgatggc cttatggttt
     5701 tttcctttga agataaagta gaaaaaatag agcctgaatt atttgtggag ttggcaaaaa
     5761 atatagaagt aaattcagaa gcatggagcc aatttataca atttgccgaa gaaaattttc
     5821 gacattcagg agataatgag cttaatgcaa aaatccctca aatattaggt ttaatcttac
     5881 acgaaagcat tgttccagag cacagggatg atatttttaa taacctttgc tcattactta
     5941 taaaatcaga aaaacccgct tttaactcaa ttacaacaag actgcttaaa gacattattc
     6001 ctgattatga aaccaaagtg atggcattcc tgcataaaaa caacaatcca atgcgttata
     6061 gcgaaaggga cattagtgag caaggagccc ttttaggtct ttcgagcgaa aaaattgacg
     6121 tagtacaaaa cgaaatgaga gaacgtaaag cattagaagt acaaaacatc aatcgatcac
     6181 aagccatcga actaaaaaaa actctcattg gtgccgtgga tgaatacctt agatggcgca
     6241 ataatgaaag caaagaagcg gattaccaaa aaagctcagg cgcttttacc tggctgcgtc
     6301 attataccga ttttggcaaa aaaagagcca atgatttaaa caagcacagg atttaaagac
     6361 catgcttgat gttgtccaaa atcatttcgc aaataactcc cgattacata atcattccct
     6421 ggatagctac ctgctaagag ccatgcacaa agattttaag aaattcaatt caatatttaa
     6481 ttttgaacct cttacaatta aaaatgatac cgatgctgac cgtgaatggc taagagaaga
     6541 aatgttggga atggcagcaa aaacaaacat gaatgttaca ttagaaaacc caacaaatag
     6601 cgggcaagag agccccactc tgcctaataa aacaaaagtc catatcagta tcatgaaaat
     6661 tccagctaac gaacgagagg aaaatatttt ggcggtccac atcgcgttaa aaaatgatga
     6721 attgctgaga aatcaagatg gatccgttcc tgctatcata aaggaaatac gagatattat
     6781 agggaaaatt gacccttcag aagaaaaaaa tatagccaat gcgattattg aaatcaaaag
     6841 aaaaatagct gataacagcg accataatta caacgagaat gcgcataaca tcatcaaagc
     6901 atttgaaagt ccaagttgtt gtgattttag aaaaatccgc gctgccttaa caaccaaccc
     6961 tgcaatggac gaaatcatga atccagtcag agtaggagtg cagttgaact aactcaattc
     7021 acataaatgt catttttacc ttcgggatct ttcccgcacc cttaccgata tgctatggac
     7081 aaaatctcgg gatataccac ttgtttttta tttttagaag tattaatcaa gaataaaaag
     7141 ttgtatgaag ctataacaca aagtacactt aatttttagc cactcgtgaa ctaaaatcca
     7201 tgaataaatc ggattctttc tacaatctgg aaaccatagg gggtatcctc ttatttattg
     7261 ctgccgtgct tgcgataatc acagccaact cgccttaccg ggttggctat gaatattttt
     7321 taagcatcaa tggcagtgtt tctgtgggaa atatgtctat aacaaaacct ctgcttttat
     7381 ggatcaatga tggattaatg gccatttatt tcctgttgat tggtctggaa ataaaacggg
     7441 aagttaatcg aggcatttta agtgacaaaa caaatctttt ggtgcctgcc cttacagcgt
     7501 tagcgggatt gctattcccc gcattaatat ttattttctt taatgctcac catcctgtgt
     7561 atctgaaagg ttgggcaatt cctaccgcta ctgatattgc ctttactctt ggaattgtct
     7621 ctcttttagg ctcacgcgta ccattctcac tcaagatttt gctgactgcc attgccattt
     7681 ttgatgatat tgcagccatt gtcattattg cattatttta taccgaacaa ttatcgttgc
     7741 tctctctttc acttgctttg gtatttactc tgattttgat tggtttaaat tattttaaat
     7801 gtcgtcgtat ttcagtcttc atgctgtttg gggtagctct atggattgcc gttttaaaat
     7861 caggtgttca tgccaccctt gcagggattg tgattgccat gacgattcct gatgagggta
     7921 aggagtccat gctgacccgt ttggaagacg gtttacatca ctgggttgtg tttttgattc
     7981 tccccttgtt tgcttttgcc aatgcagggg ttagctttgt cggacttgat gcgtccatgt
     8041 tgactcatcc tgttgtttta ggaattgggt taggattatt tcttggcaag cagttgggta
     8101 ttttcttgtc tttgggttat tttgttcaat ttaaaaaatt tcttaaagcc gataaagtga
     8161 atcttgctca ggtttatggc attgcgctca tttgtggagt aggctttacc atgagtttgt
     8221 ttattggttc tttggcttac caaaattatg atttaagttt aatgccaatg gttaaaattg
     8281 gcgtagtatt tggttctttt atagcggggt taaccggatt tttagtgtta aaaatgactt
     8341 cactcaagcg ttagttgata aggtgctttt ataaagaaaa accgatttaa ttaaggagga
     8401 tgctcatgga aaggaaaata gccattgtaa caggaggtac gggcggcatt gggactgcca
     8461 tttgtcaacg attagcagca gactattccg tcattgcctg ttacttcaaa aacggcaatc
     8521 acgaagaagc gattcagtgg caaagaacac aacaacaagc tgggcttgat atcgatatac
     8581 tgtatgctga catcgcaaag tttaatgatt gtgaaaaact ggttgccctg gtcatggaac
     8641 gctatggtcg tattgacgtt ttggtcaaca atgccggcat aacccaggat tccagtttaa
     8701 aaaagatgtc gcccgagcaa tggcagttcg tattagacgc taacctcacc agcgtgttta
     8761 acatgacacg aaatatattg cctgccatgc ttgagaaagg atatggtcgt atcatctcca
     8821 tctcttccat taacgggcgt aagggtcagt ttggacaatg taactacgca gcaaccaagg
     8881 ctgcattgtt tggctttacc aaaagcctgg ctcttgaggt ggccaacaaa ggcatcactg
     8941 caaatactat ttcgcctggc tatattgaaa cacctatgct gagcacaatg cgtgaagacg
     9001 ttttaaatac tataatttca gcaatacccg ttgggcgttt ggggctccca caagatgttg
     9061 cggaagccgt tgcctttttg gcatcacctg catcaggctt tatcacagga gccaacctgg
     9121 atgtcaatgg tggtcaatac atgtaaaaac cagagagata aaaaccaggg agcaatgatg
     9181 attcaaacag ggatttgaaa agttgctcct ctctttcaag gaggagaagt gatgcatcga
     9241 tttcacaata tcttattcgt cagtcatgga atcaaagaag aaacagaagc gttgcagttg
     9301 gcattaagac tagcagctga aagtcaggca caacttcgtg tcttaattac ttgccctcct
     9361 ttcccggata cactgagcga gtacaaaatc agctatgaag agtacttgat tgacaagatg
     9421 aacaaaacca ttcaatcggc caaatcaggt cttggtttct ctaaaaagaa aatccacatt
     9481 cctattgaaa tcgagtgtgg cagtatgccc gatgtccgaa ttattcgtca tgtccttaag
     9541 cacgctcatg accttctcat taaagaagcg gaaacaccag aaagcaaaaa aggttttaaa
     9601 gcggtagaca tggaattatt acgcaaatgt ccttgcgcct tgtttcttta tcgccctcta
     9661 aagaacacgc tgcaagacat tcgagtggca gtagcaatcg accccaaaga cgaagaatct
     9721 gttgcacagg atttatccgt tcgtttgttg gatgtgtctc attcattgaa tgccttttat
     9781 cagggtaatt tacgcattgt ttcatgctgg gattttgtac tggagaatta cttgcgtgat
     9841 agcgtttggc taaaaatctc tgaagccgaa cttgagaaaa tggtcgttga agaaaaacat
     9901 acccatggtt tactcctaag agagctgatc aaacgctctc atatttccgg agaataccac
     9961 attgagcatc tgaaaggaca tccggaagat gccatccctc accttataac ccataaaaaa
    10021 atcgatattt tagtcatggg tacggtcgct cgaaccggaa tagcgggatt tattattggt
    10081 aatacagcag aaaatatctt gcagaaaatt aattgttccc tattggcatt aaaaccacaa
    10141 ggttttgttt ctcctgtcaa agcatattaa tccgggagag gaggataatg gtggaacaac
    10201 aaaatagttc agagccagac tggcatgagc aatcagtaga aaaaacactt gttatgctaa
    10261 actccaccga acaggggata agtgatcagg aatctaaaaa gcgattagca tattacggtt
    10321 tcaatcgctt gccagaaccg aaaagaaaca gccttttttt acgcttcttg ctccagtttc
    10381 ataacatact catctatgta ttattaggtg cagctttggt gacctccttc ctgcaacact
    10441 gggtagatac caccgttata atagcagttg ttctcatcaa tgccctgatc ggttttattc
    10501 aggaaggaaa agcggaaaat gcgctcgaag cgattcgaaa catgttatct cctactgcta
    10561 ccgtaatacg cgatcgtgag aggcagcgtg ttaacggtga acaacttgtt ccaggcgata
    10621 ttattttgct ggaagcaggg gataaagttc ctgctgacgt acgcttaatc aaatcccatg
    10681 gtttgaaagt acaggaagct atcttgactg gcgaatctat tcctgttgaa aaacaaattt
    10741 ctcctgtcgc aagtaatacg gtattaggtg accggacttg catggccttt agcggaaccc
    10801 tggtaacgaa tggccagggc aagggcattg tggtcgcaac aggctcgtct acccaaattg
    10861 ggcatattag tggtttatta tcaaaagtag aatcattaac cacgccatta attattcaaa
    10921 tggggctgtt tgcaaaatgg ctcactttgt ttattttagt gcttgcttca ttgattcttg
    10981 cttatggtta ttttgttcaa catcacccgt ttggtgaatt gttcatggtt gtggttggtt
    11041 tatcagtcgc ggctatccct gaaggtcttc ctgccgtact ttccattact ttagctattg
    11101 gtgtgcaagc catggcacga cgacatacga ttgttcgccg attacctgct attgaaacct
    11161 taggctccgt atcggttatc tgtactgata aaacagggac cctgactttg aatgagatga
    11221 ctgtttcttc tgttctgaca ggtgatcatc tatttatttt ggatggtgtc ggttatgagc
    11281 ctcaaggaac aattactcta aacgaacaag tcattgataa caataaatat cctttgctta
    11341 agaaacttgc gcgcgctgca atgctttgta atgatgccat acttcacaac caggaaggca
    11401 actggactgt agaaggagac cccatggagg gtgccttact gacatttgca ggtaaaacag
    11461 gattgattct gcaagaagag aatgacttgt gggtacgaac tgatgtgatt cctttcgatg
    11521 ccagacatcg gttcatggcg accttaaatc atgatcattt gcagcacgcg atgatttttg
    11581 ttaaaggtgc tcctgagcaa attttgaaga tgtgtcaaaa tcaacaaatc aatcagggag
    11641 aagtacagcc tgtcaatgag tcttattgga aagaacaaat ggatcaggtt gctgcaagag
    11701 gacagagatt actagccttt gcggttaaaa aaacaaaacc ggagcacacc attttggagt
    11761 ttaccgatat tgagactggc ttgacattac ttgggatggt tgggttaatt gatcctccaa
    11821 gacctgaggc cattgaggct gtggcgcaat gccatactgc aggcattcag gttaaaatga
    11881 ttacaggaga tcatgccagc acagcactgg ccattggtcg ccaaatagga cttaaaaacc
    11941 tggataaagt attaacaggt cttgatttgg ataacatgga tgatgccata ctcaaaaatg
    12001 cggtgttgga gactgacatt tttgcccgaa ccagtccaga gcataaacta agactggtca
    12061 tggcattgca atcccatggg atgaccgtcg ccatgactgg tgatggtgtg aatgacgccc
    12121 ctgcgttaaa acgagccgac gcaggaattg ccatgggcag aaaaggcagc gaagtagcta
    12181 aagaagcctc ggaatttgta ctggtggatg ataatttcgc ctcgattgtc gccgccgttc
    12241 gtgaaggtcg tactgtttat gacaacctga aaaaggttat cagctggact ttacctacca
    12301 atgcgggtga agcaatgacc attattttag cgttgctttt agggctcagt ttaccagtga
    12361 cgccaattca gattttatgg attaatttaa tcaccgcggt caccttaggg attgcgctag
    12421 cttttgaacc aacggaagaa ggcaccatgc atcgtccacc gcgccctcga agtgaacctc
    12481 tattaactgg cagtttggta tggcacatta ttctggtttc cattctattc atttgtgggg
    12541 tttttggact ctatttctat gctctcgatc gcggttactc gattgaactg gcccgcacaa
    12601 tcgctttgaa tacattagtt gtacttgaaa tttttcatct gttttatatc cgtaatattt
    12661 acagcacctc actcgcatgg aaagcagtac aagggaccaa agtggtttgg atggccatta
    12721 tcgtaattac tgtggcacag tttacaatca cctatttccc acctttgcaa acaatatttg
    12781 ctactgagag catcccattt tgggatgggg tactcattgt tgcaaccggc gcaatatttt
    12841 ttgcagtgat tgaaattgaa aagcaacttc gcttaaggat aagtgctata agccacaaca
    12901 gtgcagatgc tcattatggt agtagcgtcc tatgatggat tggtcattac tgcatgcttc
    12961 tcctttttat gagctaactt cgcttctcct gttggcaacc atcataggtt ttttctgtct
    13021 gctgcttaga cagccgatga tagttggatt tattatagtt ggcatcttgg taggtcctgc
    13081 ctttcttggg attgtgcggt cccatcaaca catcgaatta ttagccgagc taggcattgc
    13141 ggtattactt tttcttgttg gcctgaaact ggatctccat ttaataaaaa ccttaggatt
    13201 ggtttccctg gccacaggac ttggacaagt cctgtttact tccttgattg gcttttttat
    13261 cgccaaaggc ctcggtatgg attcagtgac agcgctctat gtgtctgtcg cattaacctt
    13321 ttccagtacc atcatcattg tcaaactgct ttcagacaaa catgaagtag attcgctaca
    13381 tggccgtatt gcaattggat ttctgattgt tcaagacctg gtagtagttt tagccatgat
    13441 aacattgtct gcttttggta ttggcacaga gaacagtgga actgccttaa caacacatat
    13501 tactaaagtc tttttatatg gagtaacact tttaatcttt gttggtctgt tcatccgtta
    13561 tctggctaat cctttgatta atcgcatagc ccattcccct gagttattaa ttatttttgc
    13621 tatagcctgg gcagcattta tggccaccat gggcagttat tttggtttta gcaaagaatt
    13681 gggaggattg attgcgggca tctcattggc ttcgactcca atcagagaca ctattgccgc
    13741 aagactgtct tctttgcgtg acttcctatt actttttttc tttattacgt tgggctcgca
    13801 attgagttta aaccagctag gacatcaagt tactccggca attgtgtttt ctttgtttgt
    13861 cttgattggc aatccactca tcgtattggc catcatgggt gcgatgggtt atcgcaaacg
    13921 caccggcttt ctggctggat tgactgtcgc tcaaatcagt gaattctcct tggtatttat
    13981 ggcaatgggg gtcacattag gccatgtatc aacagactct ttaggattgg ttaccctggt
    14041 tggattaatt accatagctc tttcagttta catgatcact tattcacaaa ctctttatca
    14101 ttggttggag ccaattctgt ctttttttga gcgcggtaat ccctataggg aaaaaataga
    14161 aattcatcct tcaatgaaca aaaccgatta tgacattgtc atttttggtt tgggacggtt
    14221 tggtaaagcg atagcaaaat tactaattga gagaaaatta aatgtactgg cggttgattt
    14281 taatcccgat gaagtcaggg agtgggagta tcaaggatat aacgcaacct atggcgatgc
    14341 ttgtgatatt gagtttgtta cttcattgcc tttaaatcaa gtaaaatggg ttatttgtac
    14401 gattccgcag cacttgcttg gggtaactta tcaagatccc agagtgattt tgactgaaac
    14461 attaaaacaa accaaatttt ccaaccgtat cgcagtaacc actcaccaca cttctgaagt
    14521 tgaatcatta aaagccttgg gagttgatgt cattttgcag ccgttttatg acgcagctgc
    14581 cagggcagtt gaaataatcc tcgacgtatc agaaaacaac tcaataaaga ccgtaaatta
    14641 aattcgaatc ttttttctta atcactatga agatttcagc tttggaggaa caacaagcat
    14701 atccacagaa ctaagataaa gaattcgttt ggcgacactc cccatgagca agtaatgtaa
    14761 acccgatttg ccctgtgtac caaatgccag cagatccgct ttccagtgat tggcttgttc
    14821 aataatggca tcagcataat aaccgccaat aatttttttc tcaaatttgc ttttatctac
    14881 ctcacacttg ctaaggaaaa catccagttt ttcaagaata ttttttattt taggatgctt
    14941 tgtgtcggta catttctcat ccaaatcatt tggagtcaag aattggcgat aataaacatc
    15001 aacgatatgt aacaactgaa aagtagcatt gggaaaacaa tgaaaaacaa attgcagagt
    15061 ctctttgctt gcttctgaaa gatctgttgc tatcagtatc ttgttgtaag aaaaatcggg
    15121 ttctgctttt atcagcagca cgggaacatg gctttgcctg ataatcgagc cagaagtcgt
    15181 gcctaaaacg taatcattaa tatagtaagt accatgggcg ccaacaataa tcaattcaca
    15241 atgattttca tcagcatacc tgactacctc atcagaagca cggcccaata ctacagacgt
    15301 gttggcaaac agtttacgat tgttctcccc taattgcttt aaaattttct cttcgatcgc
    15361 tttcttttca gaaagatgat tgggttttaa ctcagtttct gaagattgtg cgatactact
    15421 caatggtggt gttactacat ggagaaagtg aatatgggcg ttatttaact ttgccaaacc
    15481 aatagctctt tgcaaagccc aatcagcatg ttttgagaaa tcgctggcaa tcaataaatt
    15541 attcaataaa gtcattgtct attcctcaac agaaacaatc atccttactt aagactaaca
    15601 cacaaattgc gatacgaagc aaattgttta ttatttccga aaaaccattt caaaaatcac
    15661 ggaatttatc attgaagaca atcacttatt aacctattgc ccaattctgg gttataagtc
    15721 attattggtt aggcaatcaa taagaaagta cacatttcca tgattttaag gtatggttat
    15781 tcactcacct aatgtgaaaa acatacatta caagggttgt gaacaatgac catctatttt
    15841 ttgtttggca aaactggctc gggtaaaagt tatattggag agctgctcag acagcttaac
    15901 atcgtccata ttgatggtga taaccacatc acccaaaaaa tgcgtgactg tttaatcgaa
    15961 gatgaacaaa tgactcctga gatgattgat gaatttgtcg atgtcttgat agatgttatt
    16021 aatactcaaa aagaaaaaac gcccaaagag agttttgcta tttcgcaagc gatgtatctc
    16081 gacaagcacc gtatcaagtt attaaacgcc atacccgaac tcaaatttgt aatgattgat
    16141 gtcacgcctg tactgcgggc aagtcgcatc accaaccgat ttcgcaacca ggaaagcaaa
    16201 gtaagcttac gctacgccac caaaatggat acattttttg agtatccttc acatgaaaca
    16261 attcggcttg aaaacaatca aggctcagat gaaacgctta tagagcaaat ccgtgaaaaa
    16321 atgccggcac tttttaacat ggattcaaaa gaagaattaa acccaggcat aaagcttcag
    16381 tttgcataat atccagatcc atatccagat atggttgaaa taaagaactt atgtagcaac
    16441 tgtcttgaag ttaggactta agacagttgc cacttgccag ttgggattag agaatttgac
    16501 cttttaaaaa agatattgta aaccaagtgc aacagtatag tgtttattgg atagccctgc
    16561 tgaatcaccg aaataactgc gctcacccgt gtcattgtca atgatttcag tatccgctct
    16621 cccattagaa taatgattgt aagaagcctc aacaaaaatt ttcgtattag gtttcaaaaa
    16681 gtagccagaa tgaatcgttg ccgcataata tctggagcta tttccccgct cattgaatgt
    16741 taagttgcgg gcataatgct catcatgatc tcgagctgaa acccaatcac taaattttaa
    16801 aagaccacta aactcaaaat tattaataaa atacttccct gccagaccaa tataaggagt
    16861 tctaaatttt tgttggtatc ctattcctgg ttgatctcct ggaaaacaac cggcatagag
    16921 ccctccgtaa tattggtaac aacctccagt agctttccag tcaaaggaat tccattgata
    16981 accagtagcc aggcctaact tgtaattttg tttttgcatc aaccaagccc ttaagctcat
    17041 atctaactca tttgcataat tgagatacgt atcttcatga tgagaccagt gagtccaggt
    17101 atcttgataa ggattgagcc aatcataatc atccatagca gctttatttt ttaccaaagt
    17161 tgtccagcct ctactgttca tgctaagcca atccaggaaa tcataattta attccccttt
    17221 tattatcgga gcatttttta tgcgccaatc cagctggctt aacttagtgt ctgtctctgg
    17281 atggtaaaca tattcatgag ctttacctga cagaatgcct agagaagcac ttaatgacaa
    17341 accattaaat tcgtaatccg cagcataagt agagcatgag aaaagaaagg ataaagagac
    17401 tggagccaac aaaactattt tatttttcat attttttcct agttaaggct ggcaatcctt
    17461 gcagcaaagt aactttaaaa aaccaatcgc attattgtga tccataatga tcttgtcaag
    17521 atcaacattg aataatattg atcattaagg actctaatca agcaatatca tatcgctcaa
    17581 ttgagaaagc aggagcagtt atcttagtgt ttgtattttg ggcaaccatg ttttgagtgt
    17641 aggcactggg ctttctggac aatagggcaa aaccaaatac aagcaagctg aaaactaata
    17701 aatacaacaa atcccaatac tggggaagga tatgcttacc accataatta ccaagaaatg
    17761 agaaaatacc aagtccaaaa agataaaata taaaccatag tgcttgtttg ctatcaatct
    17821 cccgatgtgt attttttata aggcaataga agaaagaaca cactactccc agaatagtga
    17881 tcaccaataa ctttcgtacg ctatggaaac ctgcccaata aaccccaact gtacaaacat
    17941 aaaatccgag aaaaccaata aggttactcc cttgtaaacg aaaaggacgc ttatagtgag
    18001 gtaattgctg acgtaagcaa accagtgcta caggccctat ggaatagcta atcattaata
    18061 ttgccacaag aaaagccgac atttcttgcc atccgtgcaa cacgagcgac atggtggcgg
    18121 cgagtaagaa atttacgcct aaactaaccc agggaacttg atacctgttt tgtttggtaa
    18181 gaattacagg tgcaggaaca actgagccca tactgcttag catgtgagct gctgtggttg
    18241 aataaacaag accagtggaa taaggtgata taaaagcatc ggcgtacaat aaaatactca
    18301 accaaagcat accggccagt gcagcgagag cggcaaatgg tcctgcatca ccagtaaaag
    18361 acaaattcac ccaccctttt tccagcgctt gttcgctaac actcccaaca aaggaccatt
    18421 gtaaaccagt gtaaagtaac gtcacgaaca ctagtgaacc acccaaaatc aggggtatgt
    18481 attgtcctgg tttctccaat tcacccatca tgataaccac ttgtcgaaac cctaaaagtg
    18541 aaaaaagcac acctccgctt gacattgctg ccataacgcc ctgccaacca taaggcataa
    18601 aaccaccgtg ctgaaaaaaa ttacccgggt ggtaactcat ggttaccaag cttgcgatag
    18661 taagaatagg gattattatc ttccagatag taaatgcggc attaatgcgg gcaaacaaac
    18721 caataccaaa ataattaaac aaaacaaaac tcattaatac aaccagggac agcatataac
    18781 ctgctggcgt atttcgatag tgcaaagcgt catgagatac cagatctgga atgtaattac
    18841 tggcatattg aatgacacct tgcgtttcaa tcactggcaa aatggcaagg gaaaaccacg
    18901 tcatgacatt catgataatg ccagtcaaac gtccatgggt atataaaggc aagcaagcca
    18961 ggctgtcgga ctgtggaaac atcgtaccta actcagcgta agacaaggca acaaacagaa
    19021 gtaaaaaacc tgccagtggc caagcgataa tggcagcagg accagcaaaa tgagcagaat
    19081 ataaagaacc aaataaccag cctgagccta ccatgctggt aattgaaatc caaagtaaac
    19141 tcaaagcact taaattttta ttcatctgat tttcacatgt ccttatgatt ttgatgcagg
    19201 caaacaattc gccagtcagc agagcgaaat cattatattt ttgcattgta aacagaaata
    19261 tcatgactac caaccctata agagtcaagt ttaaccaggt aggttacgaa taccctccag
    19321 catcaggaaa aatgaaaaac aagaactcgc agcagtgatt ttctttgcta tggattcggc
    19381 atttttcact ctgcaatctc tcgcttgaag tatctttgct tcgattgctt agctgccctc
    19441 ccttaagcta tagttaggtc tctagtgaat tatgatctgc gaccaggagt gtctatggat
    19501 ggaaaagtcg ggaatacaac aaccgggtgt ccggtgatgc atggcgggat gacatctgca
    19561 ggaacatcca acacggcatg gtggcccaat gccttgaacc tggatatatt gcatcagcat
    19621 gacaccaaga caaatccaat gggcaaagat ttcaactatc gtgaagaagt caagaaactg
    19681 aattttgagg cgctcaaaaa ggatttgcat gctttgatga ctgacagcca agcatggtgg
    19741 cctgctgatt gggggcacta cggtggttta atgattcgca tgtcctggca tgctgctggt
    19801 tcatatcgtg ttgcggatgg ccgtggcggt gctggcacag gtaatcagcg ctttgcccca
    19861 ttgaattcct ggcctgacaa tgtgaacctg gataaagcgc gtcgcttgtt atggcctatc
    19921 aagaaaaaat atggcaacaa gattagttgg gcagacttga ttgtcctggc tggcaccatc
    19981 gcttatgaat caatgggact taaaactttc ggttttggtt ttggccgcga agacatttgg
    20041 catcccgaaa aagatgttta ttggggttca gaacaagaat ggttaggcgc caagcgttat
    20101 gatgataaag accgtcaatc cctggaaacc ccgctagcgg cagtacaaat ggggttaatt
    20161 tatgtaaacc ctgaaggagt gaatggtcaa cctgacccgc tgcgcacagc gcaggatgtt
    20221 cgtgtaacct ttgggcgtat ggctatgaac gacgaggaaa ccgttgctct gactgccgga
    20281 ggacatactg ttggcaaatg tcacggtaat ggcaatgcaa aatttttggg accggagcct
    20341 gaggcagctg atattgagga ccaagggctt ggctggatta acaaaaccac cagaggcata
    20401 ggccgtaata ctgtttcaag tggtattgaa ggcgcatgga caacacaccc tacccagtgg
    20461 gacaatggct acttctattt attgctgaat tacgattggg aactgaaaaa aagccccgcc
    20521 ggtgcctggc agtgggaacc catccatatc aaggaagagg acaaaccagt agatgtcgaa
    20581 gaccccgcca ttcggcacaa tccaatcatg acagacgctg acatggcaat caaaatggac
    20641 cccgtttatc gtaaaatcgc cgagcgcttt tacaaagatc ccgactattt tgcagaagtt
    20701 tttgcacgag cctggttcaa actgacccat cgcgatatgg ggccgaagac gcgctacatt
    20761 ggccctgatg ttcctaaaga agatttgatt tggcaagatc cggtgcctgc tggtaacagg
    20821 gcatatgata ttgctgccgc caaagcaaaa attgcggcca gcaatctgac catcggtgaa
    20881 atggtttcta ctgcatggga cagcgcgcgc actttccgcg gctcagataa gcgcggtggt
    20941 gcgaatggag cacgtatacg actaaagcca caaaaagact gggaaggcaa tgagccacaa
    21001 cgcctgacta aagtcttgcg aattcttgaa gacatcgctg ctgatacagg cgcaagcgtg
    21061 gcagacgtga ttgtacttgc cggaaatgtc ggtatcgaga aggctgccaa ggcggctggc
    21121 tttgacatta tcgttccctt tgctcctggc cgtggggatg caaccgatga catgacggat
    21181 gcagaatcct ttgacgttct ggaaccgctt catgatggct atcgcaactg gcttaagaag
    21241 gcatatgatg tccgtcctga agagctcatg ctagatcgca ctcaactcat gggactgaca
    21301 gcccatgaga tgacagtcct tgttggaggt ctgcgcgtgt taggtactaa tcacaacaat
    21361 acccaacatg gtgtattcac tgatcgcgtt ggtgccctga ctaatgactt ttttgtcaac
    21421 ttgaccgata tggccaatgt atggatacca agcaaagaca atttgtacga aattcgagac
    21481 cgtaaagcgg gtaacatcaa gtggacagcg actcgtgtag atctggtttt tggctctaat
    21541 tcgattctgc gctcctatgc tgaagtctat gcccaggatg ataacaaggg aaaattcata
    21601 caggactttg ttgcagcatg gacaaaggtt atgaatgctg atcgctttga tttggcataa
    21661 tcctttgcaa gttgtcacac catagataat catagtagaa attgttccga ttatgtttag
    21721 ctttgattgc cttttatcac atcaaagcta aacatagcgt actaccataa gatgttgttt
    21781 caaaagcgaa catttacgat ggtttgcatt aggcctgatt tgcctaactt gtctattttg
    21841 ttttatttca gcgttgcaaa cgtttctgta agtctcatct actgcatgta aaccccactc
    21901 ttcaaaacgt ttacgccttg ccctaaaaca aaataatcag atcatgcaag aggtctattt
    21961 atattatgtc tcaccacaaa cgacatgctt cagcatttat atcactggaa gatgtttcat
    22021 cattgccttt gtcttctgcc ataaccgaat gcatttcaga cgatattcca ggttgtgctg
    22081 attgaaagaa accaaatgct tctttagcct ttacacttaa actacgtgaa tcacttaaaa
    22141 tttcactaca aaccaagctc tttttttcta ccatctttgc atcaagagcg ccctctgcaa
    22201 taattgatgc gcgtgcttgt attttaaatg caagctctac tcgcattaaa gcatcctctt
    22261 caatactcac attacttgat aaacatcgta tttctgtatg acttggaatc ccttttctcg
    22321 gtgtattgga ataacaaata acttgaggat atttttcttc cagaattttg tgcatattaa
    22381 ggctcattgc aataccacaa ccctcctggc taaattgttt tgcttgacag aaattgattt
    22441 tatcaatggc tggatagtga aatttagcca ataacgcatt gacctgcggg aggatatgca
    22501 ttttgtaatc ttccatgtaa tacatataag tatagtaatc aaacgaagct aataggtttt
    22561 ctgattcaat ataataaagt gaaggaatat tattttcgtc gataaataac atacacaaat
    22621 tccagtgatg aagaccacct tttgttgcca ctgggattaa tagctgccta accacagaac
    22681 tgttgtttaa cgaacacatt tcctggacat atggaagaag aactttactc acatctccat
    22741 tatttttttg attaaattca taatcctcat ctccaccagt tccaggtaga tgaataaaag
    22801 gagtgattgt ttgcaaacag gttgagttac tcttgggatg aaaattgtta agaaccatat
    22861 ggattaacaa atttacatca cggatattaa aagtttcatc catatccaga atattgttat
    22921 cctgaaaaat tatgggattt ccatcaacct ttactttaat tcttcgatgt ctgttttcac
    22981 cttcatcaac tttaggtatt tctatttcaa tttttttgtt aaatggatta ccgtccagat
    23041 caatatcttc tttatagtag gtgataattt ttgattgttt ttcataataa tcgagcggtc
    23101 tttctgaagc agagccccaa ggaatagggt agtaagattc ggtaataatt tctgtatcaa
    23161 taccgcctat ctcttttttt tccacataaa tatattggct ggatttttga tctgcatttt
    23221 gatcatttgt tgtcttattt attttcattt atttctcttg gaaaatagta atatttatta
    23281 cttcaataca ttcgctctca actattccct gatacttggc aaaaaatttc ggaatacttg
    23341 atctccaaac taattaccga tgagaaaaca ctgaagtgcc attaattttt ttatctggtg
    23401 gatttgaaat tttaaaaaac cgagggctat ctgatatagc gagatttttt cgcaccttag
    23461 agattgattt cgtataccag ggttgatacc actcattttc agcattcgtt ggcccgcagt
    23521 aacttgcttt taccgagctg aaaaactgtt ttttgttatg tattaaattg caaatccttt
    23581 tcaggttttg catttcttcc ggagaaataa aaactggcgc agtatctggc aaatcaattg
    23641 gcttgagaat ccacaatttt ggaaactatt tttttcataa atagaaattt tttataaggt
    23701 gagaccaact tccgtatgtc cacagtcagg ataaatatat tttacgcaac caaactgggc
    23761 acatttttgt gttagatcac tgtttttcgt accataaact aataaattaa ttcgtgcctt
    23821 tttcttgaat caaccatagg gcaggaagag tcaacaaagc catcaccatc aaataaatag
    23881 aaaatgcaaa atggatgaaa gcataactta atagcaaaat aatgagtggt gctgtgcctc
    23941 caaataggga attagataag ttaaaaaata aagtaaagcc actggcgcgc tcttcctgcg
    24001 gtaattgttc aaggctggcg gtaaatgcgc ttccaataaa aaaagagata atgacagcca
    24061 aaacactgca ggcgacagta atttgtaata aagtacctgt attgatcaaa taatttatta
    24121 ccaaaacggt acataaagtt gcaattaaac tgactgccaa aggaaagcgc ctgcctaaaa
    24181 tatcagaggt gaaacctgat aataagagac ccatgctata acaagtcatc cctatgataa
    24241 tgacatccat cgctgtagtt aatggcaact cctgctgttg caggtatgaa ggcatataaa
    24301 caaaaaatgt ataaaagcta ctgttgcaat aaattgccaa aagaaaaata gcagcaattg
    24361 cttttttccg ataacgccag gcattcagta tgggatgaga ttgtctttga acaattactt
    24421 ttgcaaaatc ggttaattct ctggtttgac ttcgcaaata aagtccgata aaacctacga
    24481 atattcctat taaaaatggg aagcgccaga gaaaataggg agactgactg gaaaaaaata
    24541 tcagggttat tttgcttact attattgcaa gcaacaatcc acaaatagaa cctaaagtga
    24601 cgaaactgac tgctaaacct ttatgacgcg tgttcacatt ttcataaatg taaatactgg
    24661 ctagtgtcat ttcccctgct acagaaaccc cttgagttaa tcgacatagc gtgagtaata
    24721 tcggagcgag aatgcccgcc tcttgatagg taggtaataa accaataaaa cttgatgaaa
    24781 aagtcatgac cataagagtc atagtcaatg tttttttgcg gccaattttg tcacccagat
    24841 tccctaacca ataagcagag aatggccgaa caagaaaacc tgcggcaaaa ataccaaatg
    24901 ttgcgatgag agcagtgtgt ttatcagtgg caggaaaaaa aagaggcgcc aaaatgggcg
    24961 ccataaaccc ataaatggca tagtcgtaca tttcaaacaa ggtgccaaaa gaggctgccg
    25021 caataatttt tttatccatg ttcaagctgt tgcttttgtt tcacaggtag taatacagct
    25081 tactttaact ctattgattt ctgagcttga ttctctgaat ccacttcatc cttgactggt
    25141 ataggacgac caaaaaaacc ggctatacta ggtcttgatg gtaacacaga gacaatcaat
    25201 tgtctcaatg aaactgtttc ggactcaaga ggacttggta attcgtcaag cgagagcttt
    25261 tgcggatgat tggcgatata gtcattactg tcttcgagat atttacaaat aaatgattca
    25321 taaaccccaa gacccgtatc atactttagc aaatacaaat cggcgagatg cgtgtccaat
    25381 tggccgtcac tgtttctgcc atatttggtc tcgagaaatg ttattccttg ttcacttaac
    25441 gaggcccaat gccactgttt atagatatct cttttaatac gatactcttc agggcatgga
    25501 tcaacgaaat gttcaagttg agaattggac tcaacacttt ctgcaagcgt tgcatgtcct
    25561 gcccccaagg taagatcgcc tgtatgattg atatgaattt ggtaaggggt gtcctggcca
    25621 tagttatcgc ctgggcgaat atcccaaccc gtgtagcgaa gcagaggacg aactgtataa
    25681 cgcaaagtag agaacgcacc cagatttcct tcgacggcgg cttgaaaagt ggaaaaggta
    25741 ttattcagaa tgcatcttac tgttacattt gattgcagtt tgaattgatc actgactttt
    25801 tttgccaccg cagcaccaaa ggagtcgcct tgaaaaagga tatcatcaat tgatatgcct
    25861 ttttcaagta aggtattagc cagcgctatt ccagtgttaa ccatatcatt gacttctaac
    25921 acttcaccgc ccagttttct catgcccggg tattcaaaag cgtaataatg cgcccccgtt
    25981 atttgaacag cttttgctat atcaatcagg caatcttgat attgtgttcc tattccagtg
    26041 aaataaatca catgtttccc ctttccaaca gctttgacct tctcatttag taaggagctt
    26101 gattggttgg aattattctg aaaccctatc acctcggtca tacaggtttt ttggttggct
    26161 aacgtcacct cgatctcaaa gcgctgaaaa tctataccgg ataattgttc atggtaggga
    26221 gaatccactt ggcgtattgc ctctacaaaa caggcaaaac gttcttcaag tgacatctct
    26281 gaatgaccga aaccattttt gacaaaccag tcttctctat tcggaggaaa agttcttaaa
    26341 gcaatttgtc tttgcaacca gatttgtaca cttgaattcg gcatagtccc cccttttttt
    26401 gatcaataag tgtgcataca ttttgctaat gagtcaacat ttctttgcgc atctttatta
    26461 gagataattc ttatgttcag caccgatttg aacccaattg caacaaagca aactcacttt
    26521 aatcctgcgc ttataagaaa acagtataaa caatatacaa ataattaatt ttaattatga
    26581 ccagtgggtt gatattatat tcatattgaa taaaatatgg cccgttattt aggagatttt
    26641 cataatgttt tacgaattaa tattaagcaa aagcagcaac ttgatacaag agtttagcta
    26701 tatccctcat ggtgttactt cgcttgattt gagtttgaat gaattaggca gtataagcaa
    26761 tgctgaatta attcaggcat ttaagtatat acctgaaagc gttacttcgt tggatttgtc
    26821 taacaatcac ttatgcgaca aaagcggtgc agaattggca caactcctgg ctgccatctc
    26881 cgccaatgta acttctctaa atttaagctc taattatcta gacaggaaaa gtggcgctga
    26941 gttggcgaaa gcctttgctg ctataccatc cagtgtaact tcgcttgatt tgcattgtaa
    27001 ttccctgggc aataaccgtg gggtagagtt agcaaaggct tttgcgtcta tccccgccag
    27061 cgtgacctca ctggatttaa gtatgaatta tttcgacctg gaaagtagcg ctgacttatc
    27121 gcaaattttt accagtatcc ctcctcacgt ggtgtcactt aatttgagct ttaactcctt
    27181 gcatgaagta ccctttgaaa agctagtact ccttaaggat tcgctaaagc atgtgcaaac
    27241 tgtttacttg agcttttata gtgtcaaaga aatgtccaaa gaacaacgaa gctcactggg
    27301 ctctgcattt cccaatgcgc aaaaaatcat tttggtggat gattatgaca atgaaattca
    27361 accatcaata acaatttcta acctcatcgg ggaactttct ggaaaggcag atgctccatc
    27421 cttgttaaat caatgtattc tttttgcaca aaggaatcaa atagactata tgaaaaggaa
    27481 tatccctggt gaattacaag aaagtattcg agctttcaac tctcgctgat tgataatctt
    27541 gaacaaccgt cgttcgctct tgtcaatgaa ttaagaaaca ccaaaaactt gtttgactct
    27601 tccgagtcaa acaagaaatg gaataatcga caaagaaaaa gaactctcgt taatctttga
    27661 aagttgttgg ggaattaagc attttggata tttcgccaag atgcatttct tcttcttgaa
    27721 tcatttgtct tgcaaattct tcaactaaca ccgacttatt ttctgaatat tgtaatagtt
    27781 gataatacaa atccaggctt tgcctttcat gctcaagcga ctcttctaaa atattactga
    27841 tattatgctc atgagtctct aataacttac cgattttaag tgtaggatgg taggaaaaat
    27901 gggtaataag ctcaccgatt agatgagcgt ggttcaaact ttcttgagcc tgatctcgca
    27961 tccatttgca gatcgggatg cgattaaatc caaagaccat aaaggagtag tgggtatagc
    28021 gcacaacacc cgctagctcg atctccaata atttatttag agtttttatg atggcatcat
    28081 caatgtcttg agtttcagat cttctatgtt tcataggatt acccgtcatt ttacttatgt
    28141 aagtatagaa taatttcaag aaaatctcga agtgttatca gttattgaga attgcctatc
    28201 atatctaaca taagccatga tacaaagatt acatctggca agcttggata aataaaaaac
    28261 tggctacaca aaataattat tcaattttgc caatatattg ccacttctat cattcaaggg
    28321 gcttgatgga agaaaattat gttcttttta tttactactt aaggattaat agaaaacatg
    28381 gggcataacc tgatcttaat gatctatatt aacgacaaaa acataaaatt tataaatatt
    28441 gaaaaaaaca tttgcattta actcaagatt tgcattaatc tgaatgggac tcacaaggaa
    28501 aaggagatca ccatgagtaa caaagcgaaa aacactcacc tcaaaaatgg agatagtcaa
    28561 accaaacaag cttataccaa tcaggcttca ccactttttt ttagtcaaaa gcaaataacc
    28621 ccctcatcgg gaacactgga aacaaaaaaa ccacaggaag gaaaacaacc cactcaagaa
    28681 atcagccttg agacaaccac agacatttta ttagccgcta ttttgcaaga attaaaaacg
    28741 caaaatatga ttgaactcat gaaactcaaa gctcaacagg agcaagagca agaggaattg
    28801 caagccattg agacaatgaa agagaaagat gacgccaggt ttaatgaaat ccggcaaacg
    28861 atgtatgcct gattaacagg acatccttct taaccaatta acggatgacc gttaattggt
    28921 ttgtcctctg acaagagtaa acgttaccta aaagaaaagc ccccaacctc attagatttc
    28981 ttacataatc tgtttatttt atttacgcat cacatagtag gtattcataa tgtcatgttc
    29041 caggtaatgc ttttcaattt ttccaaaacc agcttcaacc agcatttttt ctgcaagctc
    29101 ctctccccaa gccgcgccaa gacctaagcc attttgagag agagatacag tcatacagtg
    29161 catacaagag atagtgtaaa taaacgcccc cagcggatgg tgaacattcc ctgccacatc
    29221 actggaggtt ttgatatcct gcatcaggaa aataccttga ggcttcaatg ctttgaatac
    29281 gttgctcagc acctgcgctg gtttagcctg gtcatgaatc acatcaaagc tggtaataag
    29341 atcatatttc tcttgctcat cccagaccga caaatccatt ttttcaaaat ggatattttg
    29401 tattttattt tccttggctg catgtacagc atgagcaatc gtttcatcac acagatcata
    29461 accggtaaat tggctttgag gataggtttt agccattaat atcaaggcac gcccataacc
    29521 acaaccaata tcgagtacat gaattccctg gttcagtttg tcaaccaggc cgtgaaccat
    29581 agggaaaatt ttgtcagcca aggaagataa aatcgtttgt ccactctctt ccgccatgac
    29641 ttcatgaaac cgatgataag cctcataagg aacaccgccg ccgtgtttaa aagcatccac
    29701 tacgccattt tccacatagg ccaaaactgg aataaactgc gctatcactg ctatgttctg
    29761 gggtgaagat ttcctcgtaa gaaaagaagc atgttcagcg ggtaattgat aggtttttgc
    29821 atgcgcatca taattcacaa tacctcctac cacgagagca ttgagccatt ccctgacata
    29881 acgttcattg caacctgccg cctcggctat cacttcactg cttgcaaaat caagctcatc
    29941 catgacatca aacaagccgg tacgatgccc aatggatatc atcaaagtta atgcggcatc
    30001 attgagaaca gacaaaagac gttcggcaaa cgcctctgct ttggcttgat caaaacgtcc
    30061 tgttttcatc ttgcacctcc acacttagta atctttagag cactttaggg aaaactaata
    30121 aatctcaata gtgacttcga caggaactga attcgagacc acgtcatata ctggtgagaa
    30181 aggatagtat ttcttgattt cctctggtgt cgcatccgtt tttattttaa agtgaatatg
    30241 aattttttga tatccgggac ggattgactg gtccaaattg aaaatacctc gtaaatcaag
    30301 atctccctta taagaagatt ccatttcctc aatcacatgg ccattaagag cagcataata
    30361 gacaattgta gtagtcatgc agccagacag agcactaagt aaaaactcca ctggattagc
    30421 accctcatca tgaccagcca gtaaaggtgg ctctccagcc tgaaagtgaa attgtttttt
    30481 gtgatgttgc tcactggaag cagcatagaa agtatctacg taagaacggt tgctgtcgcc
    30541 atttatccat tcatttttga gcctaaactc acattgtcct aatttgggat ctttctgaat
    30601 agtgcctatg gtttttttca aagtcgcaat atcgacacca tttatcatac ttgatacctt
    30661 gtaattgtca tccatcacca tctcctgaaa acacccaaat gtacctacta ttatagtcta
    30721 tgtgatatta ctaaagggtt cttgaatgtg gcattttcat agagtcgtaa catgagtatg
    30781 atttaaccta atattggggg tattgtaact tcttggccat atcacctgat tcttatcgtt
    30841 tgatattcga ttatttttct tcaaaattca tcaaagcatt ttcatgaacc agttcatggt
    30901 gatgttctgt taatctcccc tcatagacaa tagggaattc ccattcatct ccggcgaatc
    30961 gatattcttc tgtagaaaaa tgttcaaatg catagggtat cccttggcca attaaaggag
    31021 atggtttatc aataacatga aaatgcaaat gcggtgcgtc agaattgccc gaattaccca
    31081 attttgcaat caattgattc ttacgaacca actccccctt agtgacctta atactatggg
    31141 gaattaagtg ggcatagaga gcatattggt gttttcctat tttaataatc acgtgattac
    31201 cagccatatt actcagcgta acaggataat cacgtccggg cggagtatta tcaggaacac
    31261 catcgtaaac tgcgactacc tcaccatccg caaccgaata aacattggcc ccataactgt
    31321 aatagttttc atttttattg ggattttctt tatagacata accatcaact ccaaactgca
    31381 tgaaatcaat ggcgtatcgt tcggggtaat acacaatgcc atgggctgca acccttgcgc
    31441 ggcggtgtat ggaatgattg gatgggccat taattgccat ccaatgcgtt ccatccaaag
    31501 ggttggcaat caccaggggc ttagtaattt ttacataagc gggagcagcc agaaaagaat
    31561 acctgacacc ctcttcggat attttggttt tcgatgaata aagatgaaaa cgatgcgtta
    31621 ttttttcagg gatttcatca agactatcga atttcaccat aaaaaataac aaaccacgct
    31681 cacccggtct taatgttaag tctgatgtta aatgattaga atgaccacta aaagaatata
    31741 tcatctttcg cagctcttcc tggttatagg aaaaaagaac tttataaggt tttctgccat
    31801 ttaatacttc aacgttttct aaagagaaga ggtaatcacc cacattggca agaaaaacct
    31861 catacgtgag ataaaattgt ccttcaatag tcacgggttt tggagaataa aacgtttcgg
    31921 cttctaactg aaaataaacc aatttatcct gattggattc caatccagag ggaaaagcat
    31981 gcatgtctcg tgcgagaatc aaaccactta caagaaataa aaaaattgtg acacgaaatg
    32041 attttatcat ttaaaaccct tggtaaattg ccaaaacaaa gttctatttt caataggctc
    32101 ataatcaatt ccctgtaatc aatgacaagg gttttatcat gatgagatga ttgggatcct
    32161 ggctgaaatc tgtttgctga aagcccgctt tttcgtagca acgaatggct tgttgattgt
    32221 taacatcggg gtcaacaaca acagctttga aacgggtaag aaacttatca ataaagcgat
    32281 taatgagttc cacccctaat cctctacctc gtccctcgct ttcagcaata aacaaatcga
    32341 ttccagctaa atcctgggga ttatattttt caaagagaag actctcataa ccatcaattc
    32401 catcgggtaa agagtctgtg agcaaataaa actggacgaa tccgaaaggt aagtcatctt
    32461 tatatacaat gaaacttgga acatgctcca tacctgcaat tcttggttcg tatttttctg
    32521 tgatttcatc cagtgaccag cttttgtctc tcgcatacca atgattaata attggcccct
    32581 gaaaccattg atacagttga tttaaatcct tgatgcccag aggttgaaat ttaaaaacac
    32641 tcatgacttg gctctttgaa tcccttatca atgccataac gactcacatc cgaggcatgc
    32701 caaattgatt tccatatgtc aggtttgcgg ataatcatga ttcactatac tgtaggcaaa
    32761 aactgagtgg gcattctacc acagttttca tcgcctggat ttgcttgtga ttgacataga
    32821 agatcatctc atcagtgcag gttgagcaaa ttgcccaacc gctaatctaa ttcaataacg
    32881 ctggatcaaa tggctgccca ttcactgtaa atttggtgtt ttccagagta aaagaggcct
    32941 gatagtaatc gccttcaacc tttaagatgc ctttactcac caatttttgc aagagttctt
    33001 ccgtttgttt atgggcttca gcatcgacat caatcagcgg ttctgtagcc tgagattgag
    33061 acatatccgc tgctggtgta gctgtttttg atttgattga ttcaaatagt agctgttcta
    33121 ccagtttgat tggcgcttta aactgaccat tgcctttcaa attttgcaaa agatcctcag
    33181 gattactgaa tgaactcttg gttagagcca ggttcaacga actggacaaa tgcccctgtg
    33241 gcgtatccag ctcctgattc agattgactg tcgcgccttt ggaaagcagg ttaaatattt
    33301 cagccaggat cgcgatttga ttggaaggat agtcatagtt caggtgattg agcttagcca
    33361 tcgcttgagg gtccagattc ttaacctcta tttttaactg gccaggacca tagctgttat
    33421 tgttcataac aaacttattc agggataatt gcaaattaaa atccagtaaa ttgttgttga
    33481 ctcctgccca tgcatccata cccagatttt gcaagtcaaa cactggctgt gaattagtga
    33541 cagttgaaat agaagacagc atgatagatg aatcaccaag ccataaatcg tccatgtagc
    33601 gattcaatgc aaaattgacc ttgactttcc caatccttac atcggtttct tgaacctgag
    33661 tactcatgcc gtacatagtc acatggccag aggccttatc aatcctggga ttgactttga
    33721 ggcttgcact taagccttgc cagacaaaaa caaatttgcc ttgatcaccg ttattcgtac
    33781 ccttcatatt aatcgagggc aaactataac tgacagcggt ttgatttaag taattaatca
    33841 aagctccata atgagtttcc ggtttcgtgg tgacccatgc tagcccaaaa cgcaagccat
    33901 tattggtcat aatgaaagga ccatggcgca caatcatagg aaaatccatg ctcaaatgaa
    33961 gtgctggctg aattttaata ttaccctgag cgtctttatt ttcctgggct ggcaaatcaa
    34021 caagtaaatt caaaacggca gtcgagcaga accatccact ttgattatct tttaattgcc
    34081 aagtcaagaa gggactcttg ggtatgttat cgacgttttt tttcaccgtg cttaaggcca
    34141 tcatgccaat gatagggtat gagcataaga cgataattat taacagcagc aaactgataa
    34201 tccattttct catggctgtc caatattaat ccttggtaat taatatttag actagaaaag
    34261 gttgcttgta aaatagactt atctatctga aatacccttt taagacttaa gtgagctaac
    34321 ctctcaagtt tttcttcgtg tactttcaat ttccaacctg atttttcaat aaagaaaatt
    34381 caatttaact tagcatctat aagaaaaatg tatacgaaat gcacaaaaat tgattttaat
    34441 cgcaatcagt taatatttat tgttcaggag aatttcataa tgaactacaa gctaatatta
    34501 gccacaggca gcatcctggc agaagagttc acttctatcc ctcgctatat tgtaccaatc
    34561 cagcaaaaac cggggtatta gcgggttttt acgctatttt tgcgaaaaaa atttttgagt
    34621 aaatcgaaca cgttaatacc caaagtataa tttatggcca aattattttt ttgagtgaat
    34681 ttatgtcgta catgaaaata gaagagcttc ttctaaaaga taccatgaga agagcccaga
    34741 tccctggggt ttccattgct tatgtaaatc atcaaggaat tatttccaca caagaaatag
    34801 gacttacaga tggatgcggc cttgtcaaaa tgtcaaatga tcccactcag tgcccatttc
    34861 aagagttggt tcttggatct aaaagtgaat taggtgtggg aactaaaaac acagtcatca
    34921 catttaacaa agagctttat tatgtcgacc aggcagaaaa agttgtctgg aaaatcccgc
    34981 tgacagaggc aaataaaatt gtctatgaaa tgttgcatgc aaaatgcacc gaaacctacc
    35041 aattagccga tagcaatgaa cacgaattca ttgcagctct aaccggtcgc atgccaccaa
    35101 ctgaagtgaa gcctgaaacc gtttttggcg cagcatccct aagcaaaccg gtatttgctt
    35161 atttagtaca aaaattgata caaaccaatg caaaaaatca atctgaagca ggttttgatc
    35221 aatttatttt acctgaaaaa ttaaaacatt ttgatttgga tactcccctt ttccatatta
    35281 tccccctgga agaattcaat attgacggaa tgaaatttga cctgtctgat gaatctatcg
    35341 ttgcttattc caaaatacta accgccagga tggtgttatc gcatacgact ggactggctc
    35401 atggggaaat gaaatttcaa ttcctgcccg attctgaaga aaaggaacat gggtattcga
    35461 acgttggaat tatttattta caacaagtaa ttgaaaaact gactggctca gatcttgaaa
    35521 cactgaccca aaaacatgtg tttgaaccat gcggtatgat tcatagcact tatgggctca
    35581 aggcctgcgc agccaattct ctacgcacta cagcatgtga ttatgccaat ttcgtcaagc
    35641 atttaatcca tgacagcacg attgagaatc cctttgttcc acacgcttac atgacaaaag
    35701 acaagggaca ggcaggagca atcggaataa ccaagggcaa tatacccgat gctattctgc
    35761 aacatgttgc atggggctta ggctggggac tacaaactaa tgatgaagga agagttatca
    35821 cagcctatca ttcaggcgat atgaatgatt acagagcgtg ggtagtgatc gatttacagg
    35881 ataaggataa gaaaaatgcc gtggtatttt tcgctaactc gcataatgga catattctcg
    35941 ctgaacaaat tattcctaag accatgcaaa tggaacaggc tgccaattac tttttttcaa
    36001 aatggggttt tgccagaaac ctcgctgaat taggcggaaa aactactcga ctgggtatta
    36061 aatcatcctc aggtagctct gatagctctc ctgatacttc cattcaggat gagccggttg
    36121 agataaagaa ggagacagaa aaatcagata cttcctcaat cgagacctat acttcaggat
    36181 ggacttttaa tgcttgaact gatcagtttg gctaaccagg agactccctc atcgtactga
    36241 ctcttgctat gggtcaccag ataaatagga gttggttcca gattgaaagg taaagcttgc
    36301 attcccagat taaactgtct ggcaaacata tttatcaaaa aactcggggc tgtggaaatc
    36361 accgcatcgg aattggctac tgcaaaaatt gcaggcaaga tattttttac aaaaagcatg
    36421 tcattacgta ccagcttttt tttatataaa tattcagcgg agtggctata acgctcctga
    36481 ttaaaactaa atgcgatatg tttattgctt atgtaatctt ccaaagacaa tggcctgttt
    36541 atgagtggat tgtttttgct tgcaaaacaa ataatttcct cctcatacag tttttcaact
    36601 ttgagttgcg cggatagaga attttcatta atagctccta tggccaggtc tacattctca
    36661 tcgataaata gatgtggatc atcggccatt gactgaatcg ttgtttcaat aacaatatga
    36721 ggcagttttt caagcgctgc catcagggat ggcaacaaaa ttaattcaac gtaatcaggt
    36781 aaagcgattt taaatgatct tttggtttga aagggatcaa atcgctcatt cggttgcaga
    36841 atatttctta tgaaaaacat cacttgttct aattgtggct gtaactcttt ggcttttgga
    36901 gagagaaaat attccccttt accaggaatt aatagcgggt ctttaaatag ctctctcagt
    36961 tgttttaaag aagaactcat tgccggttgg ctgacaaaca ctttctctgc agcagcactg
    37021 acgcttttct cgcttagtaa agcatccaga tgtagtatga gatttaaatt aatacgttta
    37081 aagggaatcg ccattcgatc ctctcggcaa ttgttctctt cttatattag tataagaaat
    37141 ttaaaagtat ccaatatgga taattcatta tcaagacagg gtgtattttg agaggggtta
    37201 caataccact taccgaacca ggtaacgctg tcaaatagac ctcattacct ggtatcaatc
    37261 tattgttctt tccagtgtta ttgcattagt ccaaaagatt tatctctgga agtaatgtgt
    37321 aatgttgaat cctcctcatc ttttttaacc aattgaagtt ggtagtctga aacaaacttg
    37381 gcaatcgtta ctttaaataa ggattcggtc actgcaaatg ccgggcaatt gcgcagtcct
    37441 actgaaaagg gaataaaggg gaactgacct acggtatgat ttttattgtt actgttcata
    37501 cccccctctt cagtaacttg ttctttctcc tgctgtaaga aacgggtagg gtcaaactct
    37561 tcaggattag tccagtattg ttcaccttta gctaaagcat gcaaatcaaa tatcatcatg
    37621 gtattgggtg gtatggtcat tgaatcggat gaatagccat tttgagtata tcttggcact
    37681 gcaacgggtg catcaaatcg cactgattcc ttataaaagg catctaatac aggtaaagtt
    37741 ttaagagccc ttacatctat cccttcttct atgcccaaat gcgatctgtc catttcatca
    37801 cgaagcattg caaatttatc cggatctgat gcgatttgct ccagacaaag aacaattgcg
    37861 tcagtgagat tgtctgctgc ttttaatatc atcggaagcg attgcactat aggattttca
    37921 aaatattttc taatgtcttc aatggataaa gaccttaagg cagagagcaa ttcttcattg
    37981 gaaagagtcg ggttttcatc tttaaataaa ctaaccacag aagctgcaat taaattagtg
    38041 tttcctggat taggatcatc atgaagtact tcaaccagtt tttcaaactc ttgctctaga
    38101 ataccttttg aaaaagctcg ataaacatcc cgtttttgtc tcaaactcgg cataaaattg
    38161 agtaactctg gaaaaggaaa tgccccccat cgcttgacat catttcgata agatgccatc
    38221 acatcgtagg tattgtctgg taattgcttg atgcccagca tattttccac catcactgcg
    38281 cgaacaattc cacagattgt atgtcgaagt tggatatccg catcacaatc gacacgtttt
    38341 tctacttctg taatagtggg attaaccagc cttgagggca caagaaaacg ggacaggttc
    38401 ttccgcgatg acagagcagc atgtccagta tctgaaataa taaaaaaatc attaccagaa
    38461 aaaaatttaa acgaagcatg ccctcttcgg ccagatttct cctggtcaaa ttgattcaat
    38521 aattccgtca gttgagattg agactttgga gtaatgaccg cgtagtttcc ccccaaatat
    38581 cccaaactga caacactata tcctgcttca ctgcttcgtg ctttggaagt taatgactgg
    38641 atcatcgatt taggatcacg aataaattct ctggtataac caataccaaa gcgttctatt
    38701 tgccccaaaa gacctgccac cgcctgataa gcctgattgg aataataacg aacacccaac
    38761 acgattgaac ttgcaaataa ctcaccaaac ataaatcatc ccaaacaata ggccaaaaaa
    38821 ttggactata gtaatacatt tagaaaatat tgaacacacc caggcgtttt tttatttcat
    38881 gtgggttgat tatataatgc ttattattca cccctttcaa ataccaggaa atgattccga
    38941 caccgatact tgtcaagaat tagcaggagc gatacaatga ctttgcctaa aaagttatgg
    39001 ccttcagcaa gtctcgattc ctccggtgct gggtataaag acataatttt ggtcgaggga
    39061 tactttcgtt tttaatacga ggtatcctat ggcgacgaca ttaattcaaa caccctctta
    39121 tactaaaaat ctaactctca accttgatga ctacccggga ggagttgcaa tttggggtgc
    39181 attacccgct ctttttgata cttcaaacca gggatttgat cgaggcgtcc atgttcacgc
    39241 gcgtcttgcc gattcatcta aaaaagtgat agacgcaacc tatgatcatg tcacaattat
    39301 ttccggatat cgtattttta caattacaga ggaagctgct gttcatttca gcatgtccgc
    39361 catttttgat atcaaaatta catcattaac atgtcagcac tgctctcagt tgattacttc
    39421 cgttggttac gctgcagtca ggccatcacg ccagcatcaa tgtaatcatt gtggtgaaat
    39481 cacgactaca acttcagact gcatcagcaa tccaattatg ttacttaaag aattgatagg
    39541 agatgagcaa gttaaaaggc ctgccgttat ccccaatcgt accattgcaa ttgatcctga
    39601 taaatacagt ggtggcattc aaatttgggg ctctaatcca tcaattattt ggactgctaa
    39661 aagactggaa gaaagcgcta tccatattca tgcttacaat gaaaatggca aaagaattat
    39721 agataatact tatggaagtg tttctttaga tggtcacaaa ctggatattg aaatgattag
    39781 agttctacaa atccaattgg cattgccaaa tttggcatta ctcctgacaa ccgtgtattg
    39841 tcctcattgt ggtgtggaac agtttgatcg ggggatttgg gctgtttctg cgcataatca
    39901 cagggtttgc ttactatgta aacaaacttt tatatcccaa gacgtgatca gtaaccctgc
    39961 ttttgacgta ctaacccatg tttctggagt gatctctcaa tgagcacatt aaagactata
    40021 tttaaaatag gcgcgcttat ttttaatgaa aaaaatcagt tacttgctgt acataaaaaa
    40081 ggaaaaccac ccatggagct cattgttcca ggtggtgtta tggaggaaaa tgaaagcgat
    40141 gaagagaccc tgcgtcgaga aatcaaagaa gaacttgact ccgatatcat atcatttcag
    40201 ttttataagc aatttgaaga caaggcaatc tatgaggaaa aatggctggt aatgcgtact
    40261 tacattgtca ctctcggcaa tgcccctaaa cctgctaatg agattgatca actggtctgg
    40321 cttgggcatg attatcatca aagtggctat cgttttggat ccatattagg caaacagatt
    40381 attcctgatt tctttaaata aattatagcg ggatattaaa aggatttatt catagagcag
    40441 gattcatcga gcatatcaca tcccgaaatt atgcttgcta tacttaatag tacacaacaa
    40501 gttctacggg tattatgaaa agaaaagatg agcaaaataa ccttaccaaa ggcaaggcga
    40561 atgaacaaaa tactgtcagc accaataccc atttgttatt ttcaaaacca cctggtatta
    40621 atgccgatac tgcaaatcat atgggttcat ctattgaccg cttaaaagat tttgacagct
    40681 caaaacaaag cctttggtta aaatacagtg aagaaatcat caacgaattc tgtgctttat
    40741 tacctgagtt tcccaagaaa aaacccaaag tacgtgaaga cattcgctgc ccggaagagg
    40801 cattattaac ccaggccggt tgtgcctatg cctttttagc cagtaaaaga cgcgaggtag
    40861 ccaagctttt aaaacatggg cctctaagac atcaatttgg tttatttaat aagccagggt
    40921 ttgcctcaat cgatgatttc aatctcagta aagatatccc ttctcaaaag gataaaattt
    40981 atgagcacct ctccttgtta attagtcaac ctaatccttt tattgaagtt aagaaaaacc
    41041 atagagaagc agaaggtttt tctatttaca ccataacatt caagatgaag cctgatgaaa
    41101 tagaacaagt gcacaaaaaa aatttggatt caaacaaacc ttttattgaa aatgacatgg
    41161 tactgacgat aaccttcaat gattatggga aaaatgacaa cagaacctca attgtcattg
    41221 atcactgccc tactcctttt gctgaaaact atacgagcga atccagctat ttcaaagtgc
    41281 aatttgaatt gattgagcct tactttcaag catgccaggc ctgggaagct tctgatggaa
    41341 tcgatcagtt tttaaaaaat gccggaagaa ttgcttatat actggctcgt atgcaacctg
    41401 ttggacgcgg taattctgcg attgttgaat ggatgattag aggccttgcc caaatcaaaa
    41461 gcatcgaatt aggaccattc aaccctgatg aaaaaattgg gtgggatttt aaagcgtttc
    41521 tgacgcctga aattcaggat tatgctcatt ggttttccga gaaagccttt gcgtatagcc
    41581 aattaaaaaa tgttacttcc ttgggacatt gaattaaact gagtaaacca gcctatgtgg
    41641 taaaaaaatt tatagaattg taataaatag attaaagata atcccttaga atataactgc
    41701 agatatgaag ataaaagaga acgccgggat tcaagtcttg cttttgacta taagacacaa
    41761 gaaaaatgaa agacttgccc caagataaaa gcgttattgt tttaagtttg ctttagaagc
    41821 ttcttcttca agtgcttgag cgtccaataa cctttgtgtg ttgttagtga ttgcacgacc
    41881 tgtgatcatt gattgaaagc gatcaaccat atatacacca ccccaaccga aagtgctgct
    41941 gtttttatac tcgaactcag aagctacctt gatagaatta gccagttgct catcagaagc
    42001 gcctggcgct ttatattttt tacgaatttc tttacttttt tcactggttg gtttcatgtg
    42061 ctcaacacct tcattgttaa aactgacgac attatacatg aataatattc aaacacgatt
    42121 tatttaaaaa aaacaatttg atctaaattt ttcacaaaca tttctttagt aaaaaactct
    42181 ccattacatg gcctttacaa tcacttgagt tttcaaaaaa taaaatctca caatctaaaa
    42241 acaaacccaa atagataaaa tattaacaaa ctgtatttta tttgatacaa taagtctgtt
    42301 ttaccctctg agattttttt aatgctcacc aaaaatctga atgaagcctt aagcgcaata
    42361 aaatatgtaa aatccaaact agtttatggt tccaatgccg aggaagattc aaagaagcca
    42421 aattgggagc caggaagaat tattctctta aggaaggaag ttcatgcact agcagtcgtc
    42481 tatgctcgtt cacataatgg caaagcagga tttgacgtca tctccaaatt aacccatgcg
    42541 gcactcttaa aatacggatt cggcaattgc ggtgagcaag cacaaacagc attttgttat
    42601 ctgcaatctc gaggagttgc gccgcttgat tattgtcaaa catcaatagg agaacactgc
    42661 ctgatagtga ttgggcgcgt tagtggttct gatcccaatg atatttcgac ctggggagag
    42721 gatgctgtaa tttgcgatcc ctgggctgaa aaagcctatc ctctttctga atttagagca
    42781 atgcaaagtc ccgataatga tattcgctat cccgagatat gttatgctaa tggtaaaagt
    42841 ccaaagcctt atttggcagg ggaattaatc agtcatcaca gaatagagcc tgcctcgaaa
    42901 gcatcatcac tctctttctt caaaaaagaa aattacgaac attcacaatt aaatagtgct
    42961 acccccatgc ttattgctta aagcagtcca gtgccaaagt gcaatgacta ccatgcgtac
    43021 caattgctac cttttgttgt aacgaatcat caaattatcc tgacgctatt gattaaaatc
    43081 tggcaaagag aaaaaacaca ccttgtgcga acaaggaaaa gccaggatct ctgatgctct
    43141 cttttgcaaa ataactccaa aatcatccag taagaaaaat taatccatct attgttaact
    43201 attaatattc tcttaataaa aagaaagcat tatagtcatc tggaagtcac atttgcatat
    43261 caaaaaacat aatagaaaaa caaaaccaca atcaggaggt aaaaaatgac aaacagtaac
    43321 catttaagga atcctgttac caaatcatta aatgaccttt gccaagatga gctgtctgta
    43381 attggctcct accttggtgc ctggaatgat ttatggagct tgacgtttac atcatcagaa
    43441 atgaaaaaat gcttaatcca tcagcaaacc gatgagttgt ggaaattttt tttcaaggaa
    43501 tttttccctt atattcctct accaacagag gatttttata aaaacttcat gaaaaactat
    43561 aaaacatggt atcaaacatt gtaccgtgtg aaacaggatc ctttttttct aatgtatctg
    43621 gataagctca aagaagatcc tgcacattta tttaggaatg ataaagaaat tattatagca
    43681 gccatcgaga aacaaccttt tttgtataaa aatttttccc gaggatacaa agaagacgaa
    43741 gaggttattc tgtgtgctct taaaaataat cgattcataa tccatgatgt accaagaacg
    43801 gtgagagaag gattcaaaga aatagctgat atcaaagaag ataaagcaag agttgacgcg
    43861 tgtgacaagc taattggcaa gctttcacca caacgtgttt ctaaaatcaa aagcctcgac
    43921 ttgtttaaaa gccaattaac tctgcaagcg caaaatataa caaaaaaaag tggtgaacaa
    43981 gtaaatgacc gccagcttca aagatcaact gcgcctataa cacccgaagg tatcacctct
    44041 cttgttcatt taggcctgtt taaagacaca caaatccaat atgaggaaaa ggaagaatct
    44101 ctacacaaca aatatcgaaa atgaggcgaa taaattttga taagggttgc tctatcgcaa
    44161 attcattaca gaaatagcca agcatagcct taatagattg aataaggccg ggagctaata
    44221 tattttgatt gcaaataaaa taccctaatt tttatgatac aacaccgttt tattgcgtcc
    44281 tgagtttttg gcttcataaa gtgcattatc ggcggcggta ataaggtctt gcatatcgtt
    44341 accgtgcgca gggtacatag ttaatcctat ggaaattgtg atttgagtta aagcattacc
    44401 cccataacgc aaatgtatac gactgactgc atcatgaata gattgagccc tttgtaaggc
    44461 tgcttccaga tcaatctctg gtatgatcag gataaattct tcccctccat agcggcaggc
    44521 tatatcctcc tttcgggtaa cctgacgcaa aacattagca aaagcctgta tcaccatatc
    44581 acccgcttca tgtccgaatt tatcattaaa ctgtttaaaa tgatcaatat ccaacattaa
    44641 cacggcaagt gataatgatt ttcttgcaca gcggtttaat tctcgatcca gcacttcatt
    44701 caaataacgt cgattataaa gccctgagag aacatcccgt aaagattggt tacgcaaagt
    44761 ttcacgcagc ataatattgg aaataccaag cgctatttgc tctgccatca tacttgcgag
    44821 aatttcctga ttattttctt tattgatttg ttcctctgct tcctttttaa aagatttcca
    44881 ttccatacaa accaaaccga gaatatcgct ctgagcaaat aaaggaatac aggtataagg
    44941 ctttaaatta ctgttgattt tatgatgggg gcatattaag tctctttgcg gctcttctac
    45001 tgtataaatc gagccacgac gcaaacccag gcaatcatct ggcttaatga tcctttcttc
    45061 tatcaaagag tctccccatg agctcatagc ctcaagataa ttacgtgagg gatgcatgac
    45121 atagaaaatt cctgatgact caggtaataa tttagccgca tacagagcaa ttaaatgata
    45181 ggcctccttg atatataaac aggtttgcaa tgttgaactt aactccttca accattgcat
    45241 ttctttattg cggtggttta attcatataa ggcacattca agatttttat tgacctccat
    45301 taatttccta gtcatttcta tttgagcagt tgtttctttc tggcctttct ccaattctga
    45361 ttgcagcatc acattttcca ttgcaacaga tgtgctgtca gccagtacct gcaaccaatt
    45421 taattgttcc tctgtaggaa agtaatattc tgcccaataa attccaatgg cacccaaagg
    45481 ggctgctgtt ctgataggaa ccatcgccaa actctttaca aaagtcggcc gataagcatc
    45541 aatgggaatt cttggatcag aataaatatc ttcaataatg accgactgtc gatgttccat
    45601 aacccaaccg ctgatacaag cactcatagg gaatcgtttg cctttccaaa gaggggcaat
    45661 tgcatcttcc tccgcgtaat agcattggtt gccatcacgc aatacaaagg tcgaaccgtc
    45721 agctccagtc aatgtacgag cggccttacg tacaatgaac atgattgttt ccagatcttt
    45781 tgcaagagaa agtttttgta taacatccaa taaaaactca acctgaaaat tggagccatc
    45841 agttccacaa gattttgacg gtttgaggtg tgatttgctt gggaactctt tatttgttcc
    45901 catctgtatt ctattcatgt ccgttttact ctttgctatc atccttgtct ctttgtaatt
    45961 ataataccaa atgggtgttt tttaaacaaa acatcctttg gatcactcct ctctataagt
    46021 tgtctagtaa cttaaatttt catttcgaat aattgttgaa acaagttgac gtctgctcaa
    46081 agaccattta tgttaattat tgatccatga gatcttaacc tactcaattt gatttaaaat
    46141 acccaataac tgaaacatca ataatttaaa aaaaggagag aactctcaat acaaaaacct
    46201 tgtgacaaaa tcgttttaaa tactcgatag attgtgatgg ctgtagcaaa agaatcgtaa
    46261 aaaataactt agaatttgtt ttatataagg gattatcggt atgaaaacaa agcaagaagt
    46321 ttctcagcag gacaaattaa aagattccaa atcgtctaca ccactacaga caaaagaaac
    46381 atggttcatc tctgatacct taaatattac ttttgactcc tatgatttta gtatctctgt
    46441 cactgaacaa gctcccatgc catatcgtat tgttttcagt ggcggaggct caagaatatt
    46501 ggcacatata ggtgccatgg atgagttaat caggcatgga ttacagttca ctgaattttc
    46561 cggaagctca gccggtgcaa tggtcgctgc ctttgcttat cttggttata attgttccga
    46621 aataaaacaa attatctcct ggtttaatga agataaactc cttgatagcc cactcatttt
    46681 taatttcaat aacataaaac aaatatttaa caaggggggg cttagctcgg caaaattaat
    46741 gcgtcaagca gcaaattacg ttattttaaa aaaagtaatg gatattatat ccgatgaaaa
    46801 attcaaaaca cgatttgcac aatttcaaaa ttttttagaa gaaaatatat acagctgtcc
    46861 cgagaatata acatttcaaa ccttagccag aataaaagaa atatgccctg aatgtgaatt
    46921 aggagaaaaa ctatttatta ctggaacaaa tttgagtacc caaaaacatg aagtgttttc
    46981 cattgataca acaccatcta tggcactcgc cgatgccatt attatttccg caaatttacc
    47041 tattgccttt gagcgtatct gctatcaagg aaacgtatac agcgatggag gaattagcaa
    47101 taatttacct gcccattgtt tttcagaaaa agggcataaa acaacctttt tgaaacacaa
    47161 ggatgatgtt gacttcagtg tacttgctct gcagtttgat aatgggcttg aagagaacgc
    47221 tctttatagc cagaacccaa tacctaaatg gtcatggcta agcaacacat tctactcatt
    47281 aattacagga catccaaatg ttactgaaaa ctggcatgag gatcttcaaa tactgaggcg
    47341 acatgcccat caaagtattt taattaaaac gccaactatt gctttgacca atctcacaat
    47401 tagccaagat acaaaaaaag ccctggtcga atcagggcga acagctgcca agaactatct
    47461 ggatctccat gagttttgta tggatgatta tggaaatatc agacataatg aatgcttgca
    47521 tgaaaaattt caaaaacccg aagagttatt ggattattgt gtcctacaca gccactttga
    47581 acttttaaaa aaaataaagc aagccatttc ctgctcacaa tatttagaaa aagggtataa
    47641 acattattta tgtgagttgt gtgacaactt gctcccacct caactgaaat gccccaacga
    47701 aggttcagga acagaacaac ctgaaatcaa gttagaaaaa gataccatca tttgcgaaaa
    47761 gaataataat agtgggctga cattttcaat gacatttttt ggggttccat cccctttagt
    47821 caaaacatta aataaggata gccccgaatt aaaaattaaa ctatttacag gtttatatcc
    47881 tatactaatt caaaactggc aaaatttatg ccccatttcc ggcattagtg gtatattaaa
    47941 ttctatccgc atgtcatttg ttgaaatcag ttcaacagat acctgtataa aaacattaat
    48001 tgataaactg aacgaaattg aaattggaca ttttttgata tttgttttta aagcgg
//
LOCUS       DATNSN010000008        46259 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.008, whole genome shotgun sequence.
ACCESSION   DATNSN010000008 DATNSN010000000
VERSION     DATNSN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 46259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46259
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.008"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(350..832)
                     /locus_tag="VAH76_000357"
     CDS             complement(350..832)
                     /locus_tag="VAH76_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385015.1"
                     /translation="MATYYGQCACGKIQFICTGEPVFTQYCHCNKCREIASLSQKKQD
                     KSGYSLTAAYLTRDFRIVSEENDFEEIIKEKAKLFLCSYCHSLVYGISLDPRQQDSIG
                     INVNNFKFDTNIPDSFKPIRHIWYACRIMDCDDQLPKFKDAPKEQFGSGELFELPNAN
                     "
     gene            complement(926..2905)
                     /locus_tag="VAH76_000358"
     CDS             complement(926..2905)
                     /locus_tag="VAH76_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385016.1"
                     /translation="MRYVNFELLKRIPQHLKGVMEYYPDVLLFQLNQIQYTHLWHELS
                     SAKYLYTNGFVIKPTHWLMYAFQTVKGWLGFDNHCQREKISYVLDKLSYYGYTKQFLQ
                     PDFSSITNFSISPEISALVVKSRDDLTTAQLQNKLVNNYFKVEPHLGIDYSYQKLNPN
                     HRFGESWTHAHEWGLIPQLDPQDDSLIAEVISTLDKNGISAKDVFFLQHSKYAKAAAQ
                     YYCDKAKNTIVPSFFFRLLWTDPRPRYLALALAYDPEIAKRDAQKFIEYHLAQKEYDN
                     AFNLLGILNDSNLVLKFLLAIPETERHALIQKDTAIAAIMAKYYIEKKQYLLATQFYT
                     NIEEISPNAAFAIEIQEQNYEKAYDIFKKYNSPDLFSMPERKSLAKIFYSEAETAYVA
                     GKTYRGNKSWEKAKQYYLQSLEQKKAAHHLDPSDEYLEDLYVHKRLYALLLIDADIDL
                     HKAEDSDIASIQKAITLLRECRSNNKEEQEHHKRALAAGLMRRVDTLREKIAFNYYSS
                     DFESIRKHKIEHQQDIAILIKTLGELIALLEGTQDKALRLQLGKAHYLLADVQCFFDI
                     NAPDINQHYKMAMKAVPGNPFYVLRVAELFEEEKSKLQKIGIIQLKNMGYQVFDFLHW
                     SEERWCKRDDIIHNIKDIHMPPAEPINSSSWNFTF"
     gene            3187..3981
                     /locus_tag="VAH76_000359"
     CDS             3187..3981
                     /locus_tag="VAH76_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1385017.1"
                     /translation="MATLKIHDVDIYYELYGQGKPLVLIAGYCCDHTFWNAMLHELAE
                     EFQVLIFDNRGIGQTRDNGDSFTLEAQADDIMAFLEQLGFRNPSILGQSMGGAIAQLL
                     ARKHGKKISKLVILNSVAKFNTRANQAMESLLNLRKENISFDLLIEAGIPWFFSSEYL
                     AEPKNIATFKENLKNNPYPQSLQDQVRQFRSIPPFDSRGWLHEIKVPTLVIAAEDDIL
                     TLPAESQQLAQRIPNAQFITIPGGHSSPLEYPALVNDAILKFLTSS"
     gene            3997..5463
                     /locus_tag="VAH76_000360"
     CDS             3997..5463
                     /locus_tag="VAH76_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEO1385018.1"
                     /translation="MEIYEMYIDGKFTLAKSGTTRDIFDPANGELIARVPESAKEDAI
                     LAIKAARKAFDEGEWRKSLALDRGKLLFKLADLIRANAKMLAELETRNCGKPLPEAEF
                     DVTDAANCFEFYGGLATKIHGETMSVPANSFSYVVREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTVVLKPSELTPITALELFKLIDQCGFPAGVVNLITGPGIGVGEELASNA
                     MVDKVAFTGGSATGRKIMQAATGNLKKISLELGGKNPNIVFADCDLEMAIDGALFGAF
                     ANQGEVCSAGSRLLVERSIHKKIVEGMLKKISNIKLGHGLDAGVKMGPLVSSAHREKV
                     EGYVKLGMEEGAKLLCGGKRPTGSEFAKGHFFEPTIFDEVKSTMRIAREEIFGPVLVV
                     IPFDTEEEAIRIANDTDYGLSGAVWTKSVTRAHRVTSQIRAGILWVNHYHPTYNEMPW
                     GGYKQSGAGRELGLYGIESYLEVKQVNINLDETPIGWY"
     gene            5468..6790
                     /gene="gabT"
                     /locus_tag="VAH76_000361"
     CDS             5468..6790
                     /gene="gabT"
                     /locus_tag="VAH76_000361"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEO1385019.1"
                     /translation="MKKIHIKTPIPGPKSQQLMELRRQHVARGPFHATPIFVKQAKGS
                     FVEDVDGNVFLDFSSGFGVVNTGHCPDSVVNAIKLQAEKFIHTGFNIIPYESYIKVCA
                     KLNDHTPGHFEKKSLLLNSGAEAVENAIKIARAYTGKQAVICFDHAFHGRTYMAMTLT
                     SKNKPYKHGFGPFPSEIYRAPFPYEYRWKGANCVEECFNEFTDLANFRVGVENIAAVI
                     IEPVLGEGGFIQSPALFLQKLREFCTTNDIVFIADEIQSGFGRTGNLFAMNTLGVPPD
                     LTISAKGLGGGVVLAGVTGKAEIMDAAMEGGLGGTFGGNPLSCAAALEVFHIFEEGSL
                     LQNVTHLAKALQYRLSGFKEKYKVVGDVRGLGVMQAIELVKDKNTKEPNKEAAVQLAQ
                     FCLEHGLIILTCGTYGNVIRLHMPLSTGVKDLEIGLSIIEEGLKRLSI"
     gene            complement(7218..8843)
                     /locus_tag="VAH76_000362"
     CDS             complement(7218..8843)
                     /locus_tag="VAH76_000362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385020.1"
                     /translation="MTIPLLNIENDDVKMLNRLLEKYNEQPSLYMLQLIDNHQKMLKS
                     ECIKNNPSYAQEFIRWARRDYVTAEIDALADNKFNKINTRAFETGFKEVKRDLNIEAA
                     IDKWKGIIRDMDCLKKHGERAAIDTSILKIQMAVEAIKINPKNEVHQILLVNCLSEFR
                     QYIIKELIIQENKISKLPDAIARPDRQGLKTLLKEINFQIDKIIKNHQLSEHNRFQRS
                     EVPIPELTEVCQNKTRLENLVSNLHGQENFQLGKFNLKPLGGNNNKNWLAKNNEGDCY
                     IIRLEKLLPKEILMEKIQTIPEITKHLANDFYNYPTETTYINGFGNQYNVALSEFCPR
                     KDMLSFRYDLAGQSQEKIIDEVIKMTEQVTSFALALNKAGLAYSDIKAENFLLRENKE
                     VVTADRKSIVSHDNNKDIILNGLESVAKSFTLPECREVKSNAERFMAYQIGVMLHVLM
                     EGDKVNKPHGTASYKNQDELGTIAPFDFNHDIYTTDAGKEVMKLIQNLTNENPHERLS
                     LMEVIQKCENIRENLSQNIIENQEEEERNYNFA"
     gene            9617..10549
                     /gene="glsA"
                     /locus_tag="VAH76_000363"
     CDS             9617..10549
                     /gene="glsA"
                     /locus_tag="VAH76_000363"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEO1385021.1"
                     /translation="MSSKLLTIQLLEELVHAAEFNQEGKTADYIPELANVNQELTAIA
                     VQALGEKTLAYSNNPLHPVTLQSTGKMIPLIGLLEEFGADQLFEWVKVEPSGDDFASI
                     TRLEQFGPKPSNPMLNAGAIALCSRIPGVGEQQFRWLEHWVQKLFNQRLSINPLVFAS
                     EKRTGNRNRALAYLLKSRSNLGADVHETLDLYFALCSYEAMLDQMLYLPAVLANRGQD
                     PDTGEQILSIETCKITLAIMATCGLYDETGTHMVKTGMPAKSGVSGYTIAVVPGKAGI
                     VVLSPRVNAKGNSIRGEIMLEGLSKAMGWHFALP"
     gene            complement(10651..11250)
                     /locus_tag="VAH76_000364"
     CDS             complement(10651..11250)
                     /locus_tag="VAH76_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEO1385022.1"
                     /translation="MKIVVIGATGIIGKAIVAELNPRHEIISASYKNSDIQVDITSKQ
                     SIDAMYKKLNTIDAVVLATGKVHFAALKDMGEDDYKIGLTNKLMGQVNVVLAGLEYIN
                     DNGSFTLTSGILNRDPILYGSSAAMVNGALDGFIKSAALEMPRGIRINGVSPTVITEA
                     MENYGPYFRGYEPVSAARAALAYSKSIEGAQTGQVYCVG"
     gene            complement(11581..12975)
                     /locus_tag="VAH76_000365"
     CDS             complement(11581..12975)
                     /locus_tag="VAH76_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEO1385023.1"
                     /translation="MVLEFDTIILGGGKGGKTLAMDLAKSGQKIAMVENNQIGGTCIN
                     VACIPTKTLVQSAKVAHYCRKAKDYGLNTTLHPIDFKAIRARKDAVVNGMREANLKQF
                     LDSGMDLMLGHGHFIGPKMIEVTLSSPRGNQKTLHITADKIIINTGALPYTPPIAGLD
                     KVNYFTNDSLMNTDSVPQHLLIIGGGYIGLEFAQMFRRFGAEVTVIEASSEFLGREDK
                     DIAEQVFQTLSNEGIQFAVDTKINAIRQEQTEVIIEANRQGQSEIIRGTAVLVAVGRI
                     ANTAGLHLDKTGVELDERGFIKVNEFLETTAAGIWALGDVKGGAQFTHLSLDDYRLVK
                     HNLQNPQKKLSSQNRLIPYTVFLDPELARIGLTEAQARSQGRPIKIATIPAAAIPRAK
                     TQGETTGVLKAVIDAETDLILGVSIFCAEAGEILGVIQLAMELRVPYQKLRDMMFAHP
                     TLVESINLWFAQII"
     gene            complement(13027..16389)
                     /locus_tag="VAH76_000366"
     CDS             complement(13027..16389)
                     /locus_tag="VAH76_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEO1385024.1"
                     /translation="MMTNSWKDKLQKQLTQEFGSPKGKQLIDKYTETMSMSYCEQHTP
                     EEAIQDILQIEKLSKDNPLVIDFYEKTHLQYPLHIKFYRYETPIPLSDILPMLENMGL
                     RTYTERPYKIKTRDGRLFWISDFNVTYSQTDSFNITQVNNIFAEALININLGICENDG
                     FNKLVLSAGLSWQEITILRAYAKYLHQIGIRYSQSYIEKTIEMHAAIARDLIHFFYLK
                     FGLKRKSAIQKDMSALEQKIQINIDAISSLDEDRIMRYFWSLMKATLRTNYFQKNHAG
                     LSKDYLSFKLKSSEIPDLPPGQPLYEIFVYSTRFEGIHLRSTKVARGGIRWSDRPEDF
                     RTEILGLMKAQKVKNAVIVPSGAKGGFVLKRPLHAASREQIQQEVISCYKSFINGLLD
                     LTDNLINDKTIPPQNVICYDDPDPYLVVAADKGTATFSDIANSIAKEHGFWLGDAFAS
                     GGSAGYDHKKMGITARGAWESVKRHFRELEINIQQQDFTVVGIGDMSGDVFGNGMLYS
                     KHIRLLAAFDHRHIFLDPNPDPVKSYEERLRLFNLPTSSWEDYNLQLISKGGGVYKRS
                     SKSIPISPQVKKALAIEANALTPNELIRALLKAPVDLLFNGGIGTYVKASTESHADVG
                     DKTNEFCRINGAELHCKVVGEGGNLGFTQLGRVEFALKGGLINTDAIDNSAGVNCSDH
                     EVNIKILLDKEIREKKLTEKKRNQLLSRMTDEVADLVLQDNYNQALILSISAAHSSHY
                     SGLYQDYMKELEKWVNLDRSVEFLPDDKKLLERKTTGAGLTRPELAVLMAHTKIHIAN
                     ELLKSHLPDDSYFTTFLETGFPISLRKPYAKALPKHPLSREIIATQLSNKLVNNMGIT
                     FMYRMLMETGMSIADIACAYTISACIFQTDVLQNLIDSFQDKISLSTQYELLHHIRQL
                     LNLATRWFLHNNRLTGGIEKNIKNYGKSVKKLEQLIPMLMGGVTKEYLEKLISQFVSI
                     GIEKDMAQKIAITRALYTSLNIIEVAMQNKFDLSLTAETYFKVGSQFNLVWFRDQIAN
                     DSREGHWNTMARLSLRDELDNLQRRLTIAILTTNTKERTSEKLINYWLEQNHPIQKRW
                     EKLLEMLLGSENIDYTIFFIALRELSSLIQVL"
     gene            16587..17312
                     /gene="ceg9"
                     /locus_tag="VAH76_000367"
     CDS             16587..17312
                     /gene="ceg9"
                     /locus_tag="VAH76_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg9"
                     /protein_id="HEO1385025.1"
                     /translation="MYKNILFFSNFSRMRWNEYDGDRYQSLWEHNKRDIKHTFFGTIA
                     NRKDLREVLFQPVTASFSMLVAGAFAQLLVPYSIFMLCMGEAEFAGRTLINYLEITTV
                     ALAMPFIALLSTVGRCIATVKCLVEDQYNLAEMVEDKEVKPNKIYLDCAEVNYTVRDE
                     NNNQFSRKYYKFQYKMRGLNDELCKGSFTFRDLERVPDNDDFNGKLSSLTLYKYDILD
                     LIKRVHKDKFEEMEDENLIRISI"
     gene            17545..18498
                     /locus_tag="VAH76_000368"
     CDS             17545..18498
                     /locus_tag="VAH76_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385026.1"
                     /translation="MRLSYGLLGFMIFNLVACEKMALLTTPPKKQQLSNSPLATRAER
                     YFWRTLHEGRYQDIPQADYLLMAAYLENPYDSKLAAHLGFIHIWKITERERVKNNSPL
                     LPNEIILSKKYFADALQLEPENPIYQGFYGDTQLVEGQIFKDKQEEVRGYFTLKKAIS
                     NWPEFNYFTAGYPMSSLSADSDHFKKGLDWQWKTLDICAGEKISRQNPDFTPYMKRET
                     TIGAQRACWNSEIAPHNFEGFFMNMGDMLVKSGDPETGIKIYNNAKLTKNYNKWPYKD
                     MLEKRILNAKVNVQHFNQKSNDPNQSIMFNSGYGCVVCHQR"
     gene            18658..18999
                     /gene="arsC"
                     /locus_tag="VAH76_000369"
     CDS             18658..18999
                     /gene="arsC"
                     /locus_tag="VAH76_000369"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO1385027.1"
                     /translation="MEKITIYHNPRCSKSRQALEILQNKGFEPIIIEYLKTPLVLEQL
                     KRLRTHLALEDMVRTNENVFKELGLSLANEAQVLEAMVKEPILMQRPIVTFKGKAAIG
                     RPPEKILELFD"
     gene            complement(19085..20239)
                     /locus_tag="VAH76_000370"
     CDS             complement(19085..20239)
                     /locus_tag="VAH76_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEO1385028.1"
                     /translation="MMFGFLNMTLWGYLLTGFVLTHITTTAVTIYLHRYQTHRALTLH
                     PLVSHFFRFWLWLTTGMTTAEWVAIHRKHHANTDMEGDPHSPQVFNIRKVFWQVPELY
                     REASKDKDMIKKYSYGTPNDWIERKLYSPHANKGIFLMLALNLFLFGIPGLTLWAIQM
                     MWIPLVAGVVNGIGHYWGYRNHESSDSSTNVFPWGLLIAGEELHNNHHTFASSAKFSI
                     KWWEFDLGWFYIRCLSFLKLAKVKKLPPKLIIDKNKSHIDVDSVKAILGNRFQVISDY
                     YKLVIFPTLRDMQYRIKNRDLKNARHLLKKPNEQLSHADQLRLKTLLKQHDNLQAVHN
                     YRELLQQIWLKTSLSNKDLLNLIQKWCSQAEESGLLVLRQFASQLKSYSL"
     gene            complement(20358..21602)
                     /locus_tag="VAH76_000371"
     CDS             complement(20358..21602)
                     /locus_tag="VAH76_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO1385029.1"
                     /translation="MSFKQLALIEPLNRAVSELGYTNPTSIQLKAIPLILNGHDLLGS
                     AQTGTGKTASFVLPILQKASQQTQTNRNRVKVLILTPTRELAIQVHESIIQYGKYLTL
                     RSAVIYGGVKSHNQIKQLDSGLEILVATPGRLLDLYQQGAVKFDEIDTLVLDEADRML
                     DMGFIHDIKKIIKLLPLKRQNLLFSATFTPEVRTLARNILNKAVEIDIAPRNTAVKTI
                     KQTVYSVDRNHKLALLSHLLHKNNWGQTLVFSRTKHGANKLVKQLAESQIYSVAIHGN
                     KSQAQRTKALADFKSGKVQTLIATDIAARGIDIEKLACVVNFDLPHVPEDYVHRIGRT
                     GRAGASGLAVSLVSTEEIKLLLSIEKLINQKLERIKIKDFEFLHNFSDLVSAKIQKFA
                     PPKAGYKGNISRKSRTAFNKSA"
     gene            21933..22208
                     /locus_tag="VAH76_000372"
     CDS             21933..22208
                     /locus_tag="VAH76_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO1385030.1"
                     /translation="MKQKKIYVGNLPFGTTEEALSIQFSQYGKIEEMFLIKDRLTGQM
                     KGFGFITFSAQQEAESALEMNGQPFDGRSLKVCMAQKKTNSVSRSRW"
     gene            22385..23062
                     /locus_tag="VAH76_000373"
     CDS             22385..23062
                     /locus_tag="VAH76_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEO1385031.1"
                     /translation="MKAKGFFDEQSSLQKSKIGLNLVWMFSLGHIFLITLSNVLVQYP
                     FAMMGFHTTWGAFTYPAIFILTDLTTRIATANKARKIIMLSMTPGLILSYLVASYIEA
                     GNNLNENGLSVLHLMPLRIALACFIAYVLGQLLDVAVFQRYRNRPAWWLAPTLSAVAG
                     NFIDTALFFTIAFYHCSNPFLSQNWPEIAMVDVFFKIMISLIAFVPVYGLVLSILGIK
                     TTNKLMV"
     gene            23100..23738
                     /locus_tag="VAH76_000374"
     CDS             23100..23738
                     /locus_tag="VAH76_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385032.1"
                     /translation="MLTGLRLLTKDLKILNGKYIQLEPITSQHREELRKAANHEQIWQ
                     YMPQKATGKLFNSWFDDCLEKMSARDQITYAVRCKKEGDLKGATSFYDIQPENKRLAL
                     GYSWYIPKVWGTTTNPESKLLMLYQAFDAWGINRVELGTDSRNIHSYRAIKKLGATEE
                     GVLRQHMILHNQVITDTIMFSILFSEWPAVKERLMQRLNYAAADKDHLAVSI"
     gene            23873..24400
                     /locus_tag="VAH76_000375"
     CDS             23873..24400
                     /locus_tag="VAH76_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385033.1"
                     /translation="MYNKMFKPLDTDPILYFKMYSNYTEGRVDDCCAFILMPSGLQRE
                     WVCLQSIQFAFNKRGDVLGINIIFSGNESNIHKKVRETMEGMLKLKLQYGRGEELFVF
                     DEEKKTFHMGIVPGKDTQAYLEGIIAFIKDSYRLQPDFAQDIKSQLLSKEYLAQEYSR
                     LRWKPPEKETVCVLM"
     gene            24555..25901
                     /locus_tag="VAH76_000376"
     CDS             24555..25901
                     /locus_tag="VAH76_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEO1385034.1"
                     /translation="MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQT
                     EQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHLAMANIESAQGQLRQVQLSWLPN
                     LTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
                     IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQR
                     IEAQIKVVQHNIVVTKNALHFLFNENPGDVETKILFENIDSNQLVPGNLPASVLRVRP
                     DIHEAEALLKAAHADVGAIAANLLPGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLP
                     LFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQC
                     HLAEVRYRHGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVALYQDLGGGYRGH"
     gene            25891..26928
                     /locus_tag="VAH76_000377"
     CDS             25891..26928
                     /locus_tag="VAH76_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO1385035.1"
                     /translation="MGIDQQKTVRTLRLSLTCIFLFLITWYYQVPESAWTLVTIWFVM
                     YEYSTVGGVLTKSFLRFAGTALSAIYGMIVVYFCANNPLINIMALVPGLFLYAYFFMG
                     GDKTYIGTIGAVTLTIVLLNYNDIDTAVLRVFNVIIGVIGSMFMIRFFYPQYARDKVL
                     EIQLNFITQLANLLESYLNPTQPLAAIQTKYLDYERKMLDGFTLYNRYIGEAKIETKK
                     APFFIPHSIAAMQHFRRLFRLFSVFVYYLSTEEIRSDPWVCDQLSKLLLNLQSLQRKL
                     LKQDAGPIGGDVVAAEPEEEIVIETPNIENKIATEAIVNNMQKEIALLDAEIKEIILI
                     YDVYDIRLNEM"
     gene            26985..27977
                     /locus_tag="VAH76_000378"
     CDS             26985..27977
                     /locus_tag="VAH76_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0257 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEO1385036.1"
                     /translation="MSDKPKAGFPVIILLCVFLFILIYHYSYLFPFTNNAFVVANVRP
                     VAANVKGYITNIYVKNEQEVKKGQPLFTVFKAPYELAYRKAVSDVQEAKAQLLLFNKQ
                     VEKTKYLLQAQKELYEKFRFDYEHNHSALRDHAVSKLTVNTMLKEKNAALSKLKALEK
                     ELEVNQQQVIVQKKKIDSLIAVMKNAKIDLDETTVYAKQNGAVQDMFVALGTPIKTRK
                     PVFAIADTDTLFIQANFNETDLRRVQPGDKVSIFPRMYFGSKIYHGVILSRNWAASRL
                     ETHRATQIQIVRNSESNWFLLPQRLPVQIQITDYDPVHYPLSIGASAYVYIHTH"
     gene            28153..30095
                     /locus_tag="VAH76_000379"
                     /pseudo
     CDS             28153..30095
                     /locus_tag="VAH76_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30114..31898
                     /locus_tag="VAH76_000380"
     CDS             30114..31898
                     /locus_tag="VAH76_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385037.1"
                     /translation="MKREFFQKLDKILDIASEHKQMLLQYPDVVMVGVGPERRKGKIT
                     GEPAIIVTVRKKRSSNELKKRGEKPLPQTIKGIPVDVIELGKAVEAPEIIAVQEKAKK
                     TISKIRAKWLQTPNITAIGYGYKEKGGEIDFNTIAIQFFVKKKFPMVELQKQQITPIP
                     EKINGVSTDVIQLSPICPAAASGSRGDRHDPLKGGVAIGVGSKPFHYGTYGATVFDRT
                     TGEQLFLSNQHVLDASPGTDTIQPSPVLLDDSVEVGFQLDVCLPIHFIRLDTPNTTVG
                     TVLAGAAVAAAVAAALSDEIDPTRRGQEATMPATGSKTLFETQKVKMDYPELPIPGTP
                     FKVKTAWDYERHTDAGIKTFSVSEEKKNPHVLRDKLLITDKKLYHPGDTIRIFGLILP
                     EECAPASQSRNDRQPINPRELEDITHYPNVASSINLDLVASMDSDIASSSARRTNTRA
                     AVINPKRCRCDRYHPVAILTPTTEDRAFPVVLREPVFAQKNQIFQDLLTVVKQRDDEK
                     LLERVFVLVRYGCIYTGTLRVNNIPAGPWKHFFYVQTVNIATSDMKPLLAAQIIGGLP
                     VSQNAKADLDIACGPLTWEDGEFDIELI"
     gene            32084..32482
                     /locus_tag="VAH76_000381"
     CDS             32084..32482
                     /locus_tag="VAH76_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0260 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385038.1"
                     /translation="MFGFFSDYKQYITLRNFAVVYNGLTGLAVLYSLWSNPEADPSEY
                     VIDISIHALTAITLMCKQAPESVKAVAMALNTYRGFDALFKAITSLPSTIPGIANAVD
                     VLNHRFNFKELEKLGNEETAETTSAVQHTM"
     gene            complement(32568..33206)
                     /locus_tag="VAH76_000382"
     CDS             complement(32568..33206)
                     /locus_tag="VAH76_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO1385039.1"
                     /translation="MPDVLNNYYLQTMGIEVWVMRKSTPSCEKHLAALADEVASCVRC
                     SLHKTRTQTVFSRGNPKAKLMIIGEAPGFYEDQKGQPFVGKAGFLLNKMIRSIEMTEN
                     DVYIANVLKCRPPNNRDPALEEISQCSSFLTRQIKLIKPHLILALGRFAGQFLLDKPL
                     PLKQLRNHIHYYDNTPFIVSYHPAYLLRNPSDKKKAYIDWLAVKNFLVDYGQ"
     gene            33463..34737
                     /locus_tag="VAH76_000383"
     CDS             33463..34737
                     /locus_tag="VAH76_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385040.1"
                     /translation="MNSQKDLKQLDIKQVIAWAFYDFANSSYFVVIFTFVFATYFTSY
                     IAPNTILGTELWGYTISVSALLIALVSPIVGAIADFSGHHKSWLFVFTYLGVISTGCL
                     WFAYPDSHSIPLVLTCILISNFALEVATVFYNSFLANLAPENYLGRISGWAWGCGYLG
                     GLLCLILSLFVFVKGELGGLWFGTENFANIRAVTVFVALWISVFSLPLFLVVQQKTGE
                     HLTVGTAIRKGLQELWLTIISLPKQRDLFLFLIARIFYIDGLNSILALGGIYAAGNFH
                     LSVSDIMVFGIILNITAGLGAGLFAWFDDWIGSKKTILIALACLTLTFCFLLTIHSNT
                     LFWVFAPVFGIFVGPVQAASRTFLARLAKPEEMTRMYGFYSFSGKATSFLGPFLVSTT
                     TALADSQRLGMAVLIPFFIIGGGLLLLVHENE"
     gene            34858..35514
                     /locus_tag="VAH76_000384"
     CDS             34858..35514
                     /locus_tag="VAH76_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO1385041.1"
                     /translation="MKIIISILFAFISASGHAFSCYDPQTIIQKPIQFDKKRIALTRE
                     YQLTHYGIDSESIEIEPKMIVLHWTCIPSLDTTFRIFDPPALPTSSPRRNELPGDLNV
                     SSHFLVDRDGTIYQLMPETWMARHVIGLNHYAIGIENIGGVDSKDDLTDEQVKANAFL
                     VCYLKNKYPQIKYLIGHNEYLQYKGTALWLEKDPNYQTDKTDPGPTFVKKVKQLVLAS
                     "
     gene            complement(35569..37110)
                     /locus_tag="VAH76_000385"
     CDS             complement(35569..37110)
                     /locus_tag="VAH76_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEO1385042.1"
                     /translation="MTNTINFYPIIPLAKAHWKRMSINKIKVHTLGKIFVFIMAITIM
                     ITFFNFSYATDSKLKPTVITVSEEEFQVDGKGKKTKEFKLTFTHPDGTKDNKGYFANK
                     GDMFHVLVENKSASPITLHWHGLIVPSDQDGVPDVSQILIPPGQNKLFNYRLLQAGTY
                     WMHSHQKFQEQKQLSAPLIIYDKDDPHKDLQEVILFLEDFTYKDPQLIFDQLRTAKMD
                     MKKMDSGNDLNDVTYDAFLANKKTLAAPDVISVQPSKKVRLRIINASSSTNFKIDTGK
                     LSATLIAVDGENIEPIVDSSLPIGVGNRMDLLVEIPSAGGAFPVKAMAEGTNKQTGLI
                     LKTDNTPTPVLSPNTTNKTGRVNYYELEKKLKGKNALTNKKINARLHYVLDGQMNGYI
                     WTMNKQSWPDVTPKVIHFGDRVEMIYENKSSMSHPMHFHGHVFQVTEIDGQPLDGAMR
                     DTLLVQPHSTVKVQFDALNPGIWANHCHNLYHLNAGMFTTFEYQHYPKPDFYLKTIGQ
                     PKSNN"
     gene            37339..37773
                     /locus_tag="VAH76_000386"
     CDS             37339..37773
                     /locus_tag="VAH76_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385043.1"
                     /translation="MKRIFLISIIGIVLNSAYAFEHIGECGAHTWHEVTSKSGSWKIT
                     LKMISQNGQFVDPSCAEIIETGLAQNNPLSYGLKFSKDADFNLAYTLTAVREEQGFQS
                     KTCVFVITANGPAQPDIQALSYHGAECTWRVIKGVGENFSVG"
     gene            complement(37788..38768)
                     /locus_tag="VAH76_000387"
     CDS             complement(37788..38768)
                     /locus_tag="VAH76_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO1385044.1"
                     /translation="MHAFDSIAVEFDLKNHSMQKIAIEDLNIDYQDKDKIYWIHSNLN
                     QKNIFEKLREKLQLPEQVIQLCGEKDNRSTTIEIDEALTLQIQGLCSMKLNDNYDADF
                     SNLIIHLTPNYCFTASKKLSSVLFDMLKSCPKSLPYAKTSCFLLFLLLEGVINDYAKI
                     HLAYEELADQLDAQVRTPNKNTYSQVLELKHGVMKVKRYTIAIREILMRITSRNILVI
                     SEQCRTSLYNLSNHCHLIVNEIDSLRDMLNGLLGQIDNQLMQNMNETMKVLTAFAAIF
                     LPLSLITGIYGMNFYWMPELGWKYGYFWALGLIVLCALLLFLIFRKKKWF"
     gene            39046..39642
                     /locus_tag="VAH76_000388"
     CDS             39046..39642
                     /locus_tag="VAH76_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO1385045.1"
                     /translation="MSQDMEKIEKMYQLIMEINKLKLVFRNTITNTQRNESTAEHSWS
                     ASMITLILMNELKREFKGIDELKIIKLVLIHDVVEIYAGDVLAFDLKARKDKEKVELE
                     ALEKLSAVYPSFGIELDSLWHEFEERKSLEAKIAKAADAICPIFQRLQAKQSYIPFDI
                     SMAQLEKTKYPYFQFSKTFSNLFEKLKADLLKEQLIFL"
     gene            39777..41276
                     /locus_tag="VAH76_000389"
     CDS             39777..41276
                     /locus_tag="VAH76_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEO1385046.1"
                     /translation="MYDDLWKPYASAELPSLKLGGIVLNGQLLSETPIHLVLKAFNRH
                     GLIAGATGTGKTKTMQVLAEQLSLNGVPSLVMDIKGDISGLAMPGETSTKLQDRGQQL
                     HIDFTPRGFPVELLTLTPQDPGVPLRARIDDFGPLLFSLMLDLNDTQAGVVTVLFEYA
                     KANQLNLVNLDDFKSLLQFAQTDRGNQLIAAQYGGVASTSIGTIVRKIIELESQGGKQ
                     FFGEPSFVIQDLLRTDANGYGVISILRLLNMQDKPKLFSSFMLKLLSDVYRQMPELGD
                     PDKPRLVLFIDEAHLIFNYSSKALLNLIETIVKLIRSKGIGLIFCTQTPNDIPEAVLS
                     QLGLKIQHSLRAFTAKDRKALKLVSQNFPVSSYYNTEQLLTTLSIGEALVSALDAKGQ
                     PTPLVHAMIRAPESRMGPLSNEELKVLLDQSFLFGKYSQIIDTPSAKEKLAELISEQQ
                     VAKQQTSQKENNSSVLNSLSKNTLFRQVVRQVFRELTRAILSLFKSKRN"
     gene            41357..42760
                     /locus_tag="VAH76_000390"
     CDS             41357..42760
                     /locus_tag="VAH76_000390"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO1385047.1"
                     /translation="MINITGTYTDQYQLAMAQVYFLKGHHERIATFDYFFRALPFKGG
                     YAIFAGLEDLLDVLEQLRFEQQDIEFLQHQNMHPGFIDYLQQFKFTGAVYASREGDVV
                     FPTRPVVIIEANLIEAQIIETLLLNILNFQTLIATKARRIRQVAGERILIDFGLRRAQ
                     GAGGYFASRAAIVGGFDSTSNVRAGRDYSIPVSGTMAHSFIQSYDSEIQAFSDYAEAW
                     PENCFLLVDTYNTLQSGVPNAIKIAKEMEKRGHRLKGIRLDSGDLAWLSKRARHMLDE
                     AGLNYVAITVSNQLDEYVIKNLLEQDAPIDAFGVGTNLAIGAPDAALDGVYKLAFING
                     QPSIKLSDTPAKITIPYQKQVYRVFERDGTYRGADVVCLKTEKKIDMMHHPFEILKSL
                     SIKHYKKEALLHKVMENGKRLLPKKSLGEIVRFSQQCFDRLPLEYKRFINPHIYKVGL
                     STELKMKRDQLIAEHSR"
     gene            42765..43385
                     /gene="pncA"
                     /locus_tag="VAH76_000391"
     CDS             42765..43385
                     /gene="pncA"
                     /locus_tag="VAH76_000391"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEO1385048.1"
                     /translation="MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVAS
                     QDWHPSNHISFASNHPGKKSFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEA
                     IFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
                     SQGFDCYLIEDATQALVKDDFIKIKKELIKQGTNIINSSNLLNNLQ"
     gene            complement(43467..43850)
                     /locus_tag="VAH76_000392"
     CDS             complement(43467..43850)
                     /locus_tag="VAH76_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1385049.1"
                     /translation="MSIQLNHTIVWVKDRDKSAHFLTEILGLPEAIPYGPFMVVEMSN
                     HVSLDFCKTDKEITPQHYAFLVTETEFDDIFKRICEFKMDYWADPGQTRLGQMNYNDG
                     GRGLYFNDPNGHLLEIITKPYGSGS"
     gene            complement(43850..45063)
                     /locus_tag="VAH76_000393"
                     /pseudo
     CDS             complement(43850..45063)
                     /locus_tag="VAH76_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            45181..46044
                     /locus_tag="VAH76_000394"
     CDS             45181..46044
                     /locus_tag="VAH76_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1385050.1"
                     /translation="MGKFDQITYFIAVVEEHGFAAAARKFGVSTAAVSRQIARLEAEL
                     KAELLVRTTRKLSLTDIGARYYQQCKKTLLELNEAEIAIANSRNEAVGILNVTSSRYF
                     AMRFLLPYLPEFMELNPKLQIKIELAERFPDLAQENVDVLFGVSMEGPPELVRKRVST
                     TRYVLCASPDYLAKNGIPKTPSELGKHRYITHSMRNPDNLVTFKDNEQIYVEPVLWLN
                     DSRALCECALLGMGIIKLHEYIVAEALQDGRLIEVLPEFQESSLSVYLYYQASRYLQP
                     KIRRFIDFFTK"
BASE COUNT        13618 a         8467 c         9683 g        14491 t
ORIGIN      
        1 attatagccg ctaaacttta agagaatatt aagagataaa aaacatgctg cttaagatct
       61 gatcccaatg ttttttggtt tttatcatgg atatataaag acaaaaattt tttgacagga
      121 cttacgaaaa accccaatct ctatgttaaa aaatgttttt aattcagtca gttagtttat
      181 ctaaacttcc tcattaaaaa cattttttgc cttgaccttg gggtttttcg taagtcctgt
      241 ttgaattagt tttggcaaaa cggtgttttg tcggattgaa aaatatgaga gccagatcaa
      301 tgaaatgtgc tgaatatttt ggaaagataa aggcataggg tatggtttat cagttggcat
      361 taggcaattc aaataattcc cctgaaccaa actgctcttt gggagcgtct ttaaatttag
      421 gcagttgatc atcgcaatcc ataatacggc aggcatacca tatgtgcctt atcggtttaa
      481 atgaatccgg gatgtttgta tcaaatttaa aattatttac attaatccca atactatcct
      541 gttgcctggg atcaagtgaa ataccataaa caaggctgtg acaatatgag caaagaaata
      601 actttgcctt ttctttaatg atttcttcaa aatcattctc ctcagacacg atcctgaagt
      661 ctctggtcaa ataagccgca gtgagggagt agcctgattt atcttgtttc ttttgagata
      721 aagaagcaat ttcccgacat ttattgcaat gacaatattg agtaaagaca ggctcacctg
      781 tacatataaa ttgaattttt ccacaagcgc attgaccata ataagttgcc attattctaa
      841 atgcctcctt caattggcag taatggaaaa gtatgtttta agaaagccca caaaccgagc
      901 ttattttata ctttttagct attaattaaa acgtaaaatt ccatgatgag ctattaattg
      961 gttctgcggg cggcatgtgg atatctttga tattatgaat gatatcgtct cgtttacacc
     1021 aacgctcttc agaccagtga agaaaatcga agacctggta ccccatattt ttcagttgaa
     1081 ttatgccgat tttttgcagt ttgctttttt cctcttcaaa tagctctgct acgcgtagca
     1141 cataaaaggg gtttcctggt actgctttca tcgccatttt ataatgctgg ttaatgtctg
     1201 gggcattgat atcaaaaaag cattgaacat cggccaggag ataatgtgct ttacccagtt
     1261 gcaagcgcaa ggctttgtct tgagttcctt ccaatagagc aattaactcc cccaatgttt
     1321 taattaaaat cgcaatatct tgttggtgtt cgattttatg ttttctgata gactcaaaat
     1381 cagaagagta ataattaaaa gcaatttttt cacgcaaagt atctactcgg cgcataagtc
     1441 cggctgccag ggcgcgctta tgatgttctt gttcttcttt attgtttgag cggcattccc
     1501 tcaacagagt gattgctttt tggatgctgg caatatcgct gtcctctgct ttgtgcaggt
     1561 cgatatccgc gtcgattaat aacaaggcat ataagcgttt gtgaacgtat aaatcttcca
     1621 ggtattcgtc agagggatcc aggtggtgtg ctgccttttt ttgctccagg ctttgcagat
     1681 aatattgttt tgctttttcc caacttttat ttcctctata tgttttcccg gctacgtagg
     1741 ctgtttcagc ttcactataa aatatcttgg caagcgattt acgctcaggc attgaaaata
     1801 ggtcagggga attgtatttc ttaaagatat cataagcctt ttcataattt tgctcttgaa
     1861 tttcaatagc aaatgctgca ttagggctaa tttcttcaat attggtgtaa aattgtgttg
     1921 ccaggagata ttgttttttt tcaatataat atttcgccat gatggcggcg attgctgtgt
     1981 ctttttgaat taaagcatga cgttctgttt ctggaatcgc taacaaaaat tttaaaacca
     2041 gattggagtc gttcaatatg cccaatagat taaacgcatt gtcatattcc ttttgcgcaa
     2101 gatgatattc aatgaatttc tgggcatccc gcttggctat ttcaggatcg taagcaaggg
     2161 ccaaagcgag atagcgaggg cgcgggtcgg tccaaagcaa gcggaaaaaa aaggaaggga
     2221 caattgtatt tttagcctta tcacaatagt actgagcagc agctttggcg tatttgctat
     2281 gttgcaaaaa gaacacatct tttgctgaaa tcccattctt atccagggtg ctgattacct
     2341 ccgcgatcaa gctgtcatct tgagggtcta attgcggaat cagtccccac tcgtgtgcgt
     2401 gcgtccagct ttcaccaaag cgatgattgg ggtttagttt ctggtaggaa taatcaattc
     2461 caagatgggg ctccacttta aaataattat tcaccaattt attttgtagc tgggctgtcg
     2521 ttaaatcatc tctgcttttg acaaccaggg cagaaatttc aggagagatt gagaaatttg
     2581 ttattgatga aaaatcaggc tgaagaaatt gtttcgtgta tccataatac gagagtttgt
     2641 ctaaaacgta tgaaattttc tctcgctgac aatgattatc aaagccaagc cagcctttaa
     2701 ccgtttgaaa ggcatacatc aaccaatggg taggttttat cacaaatccg ttagtataga
     2761 ggtattttgc ggaagataac tcatgccata aatgggtata ctgtatttga ttcaattgga
     2821 acagaagcac atccgggtag tattccataa cccctttaag atgctgaggg attctcttta
     2881 ataattcaaa attaacataa cgcatttatt ttcatctaaa atagatagga aggcgatgaa
     2941 gcgatgagta tattaaacct taattaagaa attattaaga ggaattgatt tctgcatcaa
     3001 ttgtcttgat ggtaattgag cagtcagatt ttataaaaac taacaattat gactgcagga
     3061 acttatcaaa cacgtttgtt gacaaatgac ttaattcatc gaaacaatag ataattgagg
     3121 cgaaaagtca ttgaatgaag cgttgcgttt gttcgtcaaa aaaatcaatt aaggagtttt
     3181 tgcgacatgg ccacactcaa gattcatgat gttgatattt attatgagct ctatgggcaa
     3241 ggtaaaccac tggttttaat tgcaggctat tgctgtgatc acaccttttg gaacgccatg
     3301 ctccatgagt tggctgaaga attccaggta ttgatatttg acaatcgcgg gatagggcaa
     3361 acgagagaca atggagattc ttttacatta gaagctcagg cggatgacat catggctttc
     3421 ttggagcaat taggttttag aaacccttct attctggggc aatcaatggg tggggctatt
     3481 gctcaattgc ttgccagaaa gcatggtaaa aaaatcagta aattggttat tttaaattcg
     3541 gttgcaaaat tcaatacgag agcaaaccag gctatggaaa gccttttgaa tttgcgaaag
     3601 gagaatattt cattcgattt attaatagag gctggaatac catggttttt ttcaagcgaa
     3661 tatttggctg agcctaaaaa tatcgctact tttaaagaga atttgaaaaa taatccttat
     3721 ccgcaatcct tacaagatca agtgcgacaa tttagaagca taccaccatt tgattcccgc
     3781 gggtggctac atgaaattaa agtgcccaca ctagttatag cagcagagga cgatatactg
     3841 accttgcctg cagaaagtca acaactggca caaaggatcc ctaatgctca attcattaca
     3901 attcctggcg gacattccag tcccttggaa taccccgcgt tagtcaatga tgccatacta
     3961 aaatttttaa cctcttccta aaaaggtgct gaataaatgg aaatttacga aatgtacatt
     4021 gatggaaaat ttactttagc caaaagcgga actactcgcg acatttttga tccggctaac
     4081 ggggaattga ttgcaagagt gccagaaagt gccaaagaag atgccatact tgctatcaaa
     4141 gcagcgagaa aagccttcga tgaaggagag tggagaaaat cactggcgct tgatcgaggc
     4201 aaattgttat tcaagcttgc tgatttgatt cgagccaatg ctaaaatgtt ggcagagctg
     4261 gaaactagaa attgcggaaa gccgttaccc gaggcggagt ttgatgtcac tgatgctgcc
     4321 aattgctttg aattttacgg tgggcttgcg actaaaattc atggtgaaac gatgtcggtc
     4381 ccagcgaatt catttagtta cgtagtacgt gaacccatag gcgtgtgtgg gcaaatcatt
     4441 ccatggaatt ttccactgct tatggcggcc tggaagcttg cacccgcact tgccgcaggc
     4501 aataccgtgg tattaaaacc atcagaattg accccgataa cggcacttga gttattcaag
     4561 ttgattgatc aatgcggatt tccagcgggc gtggttaacc tcattactgg gccgggtata
     4621 ggagttggag aggagttggc aagtaatgct atggtggata aggttgcttt caccggagga
     4681 tcggcaacag gcagaaaaat catgcaggca gctactggca atttgaaaaa aatctcgtta
     4741 gagcttggcg gaaaaaatcc aaatattgtc tttgcagact gtgaccttga aatggcaatc
     4801 gacggtgccc tttttggtgc ttttgcaaat caaggggaag tctgttctgc gggttcccgc
     4861 ttgctagtgg agcgctctat tcacaaaaaa atagtagagg gaatgttgaa aaaaatttca
     4921 aatatcaagc ttggccatgg tttggacgct ggggtaaaaa tggggccttt ggtttcaagt
     4981 gcacatcgag aaaaagtgga aggctatgtc aagcttggaa tggaagaagg ggccaaatta
     5041 ctttgcggcg gtaaacgccc gacaggcagt gaatttgcaa agggacattt tttcgagcca
     5101 accatttttg atgaagtgaa atccacgatg cgtattgctc gtgaagaaat ttttggtcca
     5161 gtacttgtgg tcatcccctt tgatacagaa gaagaagcca ttcgcattgc gaacgatact
     5221 gattatggtt tatccggggc tgtatggacg aagagcgtta ctcgtgccca tcgagtcaca
     5281 tctcaaatcc gtgccgggat tctttgggtg aatcattacc atccaaccta taatgaaatg
     5341 ccttggggcg gatacaaaca aagtggagcg ggtcgtgagt taggcttgta tggcattgag
     5401 agttaccttg aggtcaagca agtgaatatt aacctggatg aaacaccaat tggttggtac
     5461 taaactaatg aaaaaaattc atatcaaaac gccaataccc ggacccaaat cgcaacagtt
     5521 gatggaattg cgacgtcagc atgttgcaag agggccattt catgctactc ctatctttgt
     5581 gaagcaggcc aaaggttctt ttgtggagga tgttgatgga aatgtatttt tggacttttc
     5641 atcagggttt ggggtggtca atacagggca ctgtcctgat tcggtggtca atgccatcaa
     5701 actccaggcc gaaaaattta tacacaccgg cttcaatatt attccctatg aatcttatat
     5761 caaggtgtgt gcaaaattaa atgatcacac ccctggacat ttcgaaaaaa aatcgctcct
     5821 tttgaattcc ggtgcggaag cagttgaaaa tgccattaaa attgctcgtg cctatacggg
     5881 caaacaagct gtgatttgtt ttgatcatgc ttttcatgga cgcacgtaca tggcaatgac
     5941 actcacttcg aaaaacaaac cctataagca tggattcggc ccgtttcctt ctgaaatcta
     6001 tcgtgctcct tttccttatg agtatcgttg gaagggagcc aattgtgtag aagaatgttt
     6061 caatgagttt accgatcttg ccaatttccg tgtcggtgtt gaaaacattg ctgcagtcat
     6121 tatagagccg gtattgggtg aaggtggatt tattcaatcc cctgccttat ttttgcaaaa
     6181 acttcgtgag ttttgtacga caaacgatat cgtttttatt gccgatgaaa tccaatccgg
     6241 ttttggccgc acaggaaatc tgtttgcgat gaatacgttg ggagtacccc ccgatctcac
     6301 catatcagca aaaggccttg gcggcggggt agtgcttgct ggtgtaacag gtaaagctga
     6361 aatcatggat gcggctatgg aaggtgggtt gggtggaact tttggtggaa atcctcttag
     6421 ttgtgctgct gctcttgaag tgttccatat ttttgaagaa ggaagcctgc tgcaaaatgt
     6481 tacccatctt gcaaaggcac tgcagtacag actttctgga tttaaggaaa aatacaaggt
     6541 agttggtgat gttcgtggtt tgggtgtcat gcaggcgatc gaattggtaa aggataaaaa
     6601 cacgaaagaa cctaataaag aagccgcagt gcaattggct caattttgtt tagaacatgg
     6661 gttgatcatt ttgacctgcg gaacatatgg gaacgtcatt cgtctccaca tgccactttc
     6721 tactggcgta aaagatttgg aaatcggcct ttcgattatt gaggaagggc tcaagcgact
     6781 atcaatatag atatagagtt tgaaatgaaa aagcccactt aacatgcact tagaagcctt
     6841 ctcgtctcaa ctctacgtcc atgagagcaa gcaaattgca tcgactgggt gtatttaagt
     6901 tattttcgat ttcagatgaa gaagtaacca aacccgtttg tttatcaagc caaataagat
     6961 actaatatag cgacctccgc cattttgttg ccttaatatt ggctataaaa cgggcgtatt
     7021 gagcgccggg gagtagcatt gagttgatgt gagctcaaat gagctttgtc ttcttgtttc
     7081 cttgacttta ctaatgaaca acaacccgat ggtacgagtc catagggttg ttgttcagat
     7141 taacaccctg tatgaggagt ttttttcatg catgctaata gcaaaaggtc actagtttag
     7201 tttcaaaaga gccatggtca tgcaaagtta taattgcgct cctcttcctc ctgattttct
     7261 ataatatttt gagatagatt ctcccttatg ttttcacatt tctgaatgac ttccattaat
     7321 gacagccgtt catgtgggtt ttcattagtc agattttgta ttaacttcat gacctctttt
     7381 cctgcatcag ttgtgtaaat atcatgatta aaatcaaagg gcgctattgt acctaattca
     7441 tcttgattct tataggaagc ggttccatga ggtttattca ctttatcccc ttccattaaa
     7501 acatgcagca ttaccccaat ctgataggcc ataaatcgct ctgcgttact ttttacttcc
     7561 ctacattctg gtaaggtgaa tgattttgct acactttcta gcccattaag tataatatct
     7621 ttgttattat catgagaaac aatggatttt ctgtcggctg tgacgacctc tttgttttct
     7681 cttaataaaa aattctcagc tttaatatct gaataggcca atcctgcttt atttaaggcg
     7741 agcgcaaaac tagtcacttg ttccgtcatt ttaattactt catctataat tttttcttgt
     7801 gattgtcctg ccaaatcata gcgaaatgag agcatgtctt ttcggggaca aaactctgag
     7861 agtgccacat tatattggtt tccaaatcca ttaatataag tagtttctgt agggtagtta
     7921 taaaaatcat tggccaaatg ttttgttatt tccgggatag tctgaatttt ctccatcaag
     7981 atttcttttg gcagaagttt ttccaatcgg ataatatagc agtcgccctc attgtttttg
     8041 gcaagccagt ttttgttatt gtttccccct aaaggtttta aattaaattt acctaactga
     8101 aagttttcct gtccatgaag attggaaacc aagttttcca accgagtctt attctgacaa
     8161 acttctgtca attcaggtat cggtacttca gaacgttgaa accggttgtg ttcacttaac
     8221 tgatgatttt ttatgatttt atcaatctga aaattaatct ctttcaatag cgtttttaag
     8281 ccttgtctat cgggtcttgc aattgcatca ggcagtttac ttattttgtt ttcctgtatg
     8341 ataagttctt ttattatata ttgccgaaat tcgctgaggc aattgaccaa aagaatctga
     8401 tgcacttcat tttttgggtt tattttgata gcttctacag ccatttgtat ttttaatatg
     8461 gaggtatcta tagcagccct ctcaccatgt ttcttgagac aatccatatc tcttattatt
     8521 cctttccatt tgtctattgc tgcttcaata tttaaatccc ttttaacctc tttaaaaccc
     8581 gtttcaaaag cgcgagtatt tattttattg aatttgttat ccgcaagcgc atcaatttct
     8641 gcagtcacat aatcgcgtct tgcccatcta ataaattctt gcgcataaga ggggttgttt
     8701 tttatgcatt cagactttaa cattttctga tgattatcaa ttagctgtaa catgtataat
     8761 gaaggttgtt cattatattt ttctagcaaa cgatttagca ttttaacatc atcattttca
     8821 atattgagta atggtatagt catggcgtac tcccgctggt tgtgttttta ttttaaccag
     8881 ccagtacaat attgatccag cgaattgtat tgatatgtct tagcaatttt ttaaatatca
     8941 ctttattttc aatgtattaa taaggcttat ggattttttg agacgaaaaa tctggagaag
     9001 gtgcaaggtt tcgctttgag aagtaaaata attttttcta aaaccagtta ggggatcctg
     9061 ttagcgttct taagattatt cctccaaagc aggcataagc atccctacct ttttatttcg
     9121 cttactggaa ataaattcat ggatggattt atggggattg aggctctata gattggtttt
     9181 aaaggtttta tacgatatgg ggactctccg cagtgagata atagtctgca attaaaagtc
     9241 tactgtatta attattaaag cttgtcaatt tttctttttc tctatgctca atatggatat
     9301 aaggtggata aattttgggt gaaatcaagt gatttttgcc aattcttatg gttttcgaca
     9361 ctatgaattt cgattattag ctattgaaaa tagcgaatta tttttggaga tcaagagagg
     9421 ggtatttctg ctttcctagt actaaagact tgaataaaac tattattcgc gacccagggc
     9481 taaatttttt tggccgagtg attttaaaat caatcccatt tcattcttaa ccgatttatt
     9541 aataatatta aaataaaata tttgtcttaa tgtgctaaat ttaccataat agcccccttt
     9601 ttaatccaat agtactatgt catcaaaatt gcttactatc caattgttag aagagcttgt
     9661 gcatgctgcc gaatttaatc aagaaggcaa aaccgcggat tatatccctg aactggcgaa
     9721 tgttaatcag gaattgacgg caattgcggt gcaagctttg ggtgagaaaa ccttggctta
     9781 tagtaataat ccacttcatc cagtgacttt gcaaagcacg gggaagatga tccctttgat
     9841 tggtttgctt gaggagtttg gtgctgatca attgtttgaa tgggttaagg tggagccttc
     9901 gggtgatgat tttgcttcca ttacccgcct ggagcagttt ggacctaaac cgtcaaatcc
     9961 catgctgaac gctggcgcaa ttgccttatg ctcacgtatt ccaggcgtgg gtgagcagca
    10021 gtttagatgg ttagaacatt gggtacaaaa attatttaat caacgtctca gtattaatcc
    10081 tctggttttt gcctctgaaa aacgaaccgg taatcgtaac cgcgctcttg cctatttact
    10141 gaaaagcaga agcaatcttg gagctgatgt tcatgaaacc ctcgatttgt attttgcgct
    10201 atgttcctat gaagccatgt tagatcagat gctgtactta cctgctgtgt tagcgaatag
    10261 agggcaagat ccggacacgg gtgaacaaat cctttctatc gaaacctgta aaattacttt
    10321 ggctatcatg gcaacctgtg gattatatga tgaaacaggt actcatatgg ttaaaacagg
    10381 gatgccagct aaaagtggag tgtctggtta taccatcgcg gttgttcccg gtaaagccgg
    10441 tattgtggtg ttgagtccaa gagtgaatgc gaaaggaaat agcatacgag gtgaaataat
    10501 gctggaaggt ttgtccaaag caatgggttg gcattttgcg ttaccttaat tttaagtcct
    10561 gtaaataaga taacgctgta tggtgttata aggaaaaatt gaaatatggt ttgtagtgct
    10621 tcagacgtga tttaactcag cattttaatg ttatcctacg caatacactt gacctgtttg
    10681 agcgccctca atactcttgc tgtaggcgag tgctgcacga gcagcggata caggttcata
    10741 gcctctgaaa taggggccat agttttccat ggcttcggta attactgttg ggcttacacc
    10801 attgattcgg atacctcttg gcatttccag agcagcactt ttgataaaac catccaatgc
    10861 gccattaacc atggcagcgg aacttccata gagaatggga tcacgattta atattccgct
    10921 ggttaatgta aatgagccat tatcgttgat gtactctaat ccagccaaga ctacattaac
    10981 ttgacccatt agtttattgg tcaaaccaat tttataatcg tcttctccca tgtcttttag
    11041 ggcagcaaag tgcactttac cggtagctaa cacaaccgca tcaatagtat ttaatttttt
    11101 gtacattgca tcgattgatt gtttactggt tatatccact tgaatatcac tgtttttata
    11161 gctcgctgaa ataatttcat gcctcggatt taattcagca actattgctt ttccaattat
    11221 ccccgtagca ccaatcacca ctattttcat aattcattcc ctctttatcc aataaaaagc
    11281 aaaagtccta cgtagaataa tacagtaaca acaatgagtt aaatacagga cttacgaaaa
    11341 accccaatct ctatgttaaa aaaagttttt aattcggtca tttagtttat ctaaactccc
    11401 tcattaaaaa cattttttgc cttgaccttg gagtttttcg taagtcctga aataaaaagt
    11461 gtgtatcggc tgtatttgat tactgcctat acacaggatt tattgaagaa gtgaagtaaa
    11521 gtgcctctat taaccgcttg ccttgtagca tgtaatatgg cgagtatggt ttacatcaat
    11581 tcaaattatt tgcgcaaacc acagattgat actttctacc agagtagggt gagcgaacat
    11641 catatcgcgc aatttttgat aaggtacgcg caactccatt gctaattgga tgacccctaa
    11701 tatttcccct gcctccgcac agaaaataga aacgcccaaa atcaaatcgg tttcagcgtc
    11761 aatgactgct tttaaaacac ccgttgtttc tccttgggtt ttggcgcgag gaatcgcagc
    11821 tgctggaatt gtggcgattt taattgggcg gccttgagat ctggcttgag cttcagtaag
    11881 tccaatgcgt gctaattccg ggtcgagaaa cactgtgtaa ggaataagcc tgttttggga
    11941 agaaagcttt ttctgggggt tttgtaaatt atgtttcacc agacgatagt catcaagaga
    12001 caggtgggta aactgtgccc ctcccttgac atcgcccaat gcccaaatcc ctgccgctgt
    12061 cgtttcgaga aactcattga ctttgatgaa tccacgctca tcaagttcaa caccagtttt
    12121 atccaaatgc aatcctgctg tattggcaat acgtcctact gcaaccagga cagctgtgcc
    12181 acgaataatt tctgattggc cttgtcgatt cgcttcgata atcacttcag tttgctcttg
    12241 gcgaatggca ttaatctttg tatcgacagc aaattgaatg ccttcgttgg ataaggtttg
    12301 aaaaacctgt tcagcaatgt ccttgtcttc ccggccaaga aactcgctgc tggcttcgat
    12361 gacggtgact tcagcgccaa accggcgaaa catttgtgca aattccaagc caatataacc
    12421 gccgccgatg atcaataaat gttggggtac cgaatcggtg ttcattaaac tgtcattggt
    12481 aaaataattg actttatcaa gccccgcaat ggggggagtg taaggcaggg cgcctgtatt
    12541 aataataatt ttatcagctg tgatgtgcag tgttttttga ttgccacgtg gtgaggatag
    12601 ggtaacttca atcatttttg gtccaataaa atgaccatgt cctaacatga gatccatgcc
    12661 cgaatctaaa aattgtttta aattcgcttc acgcatccca ttcaccacag cgtctttgcg
    12721 tgctcgaatt gctttaaaat caattgggtg caatgtagta tttaaaccat agtcttttgc
    12781 tttacggcag taatgcgcca cttttgctga ctgcacaagc gttttcgtag gtatgcatgc
    12841 cacattgata caggtcccgc cgatctgatt gttttcaacc atcgcgattt tctggccgct
    12901 tttagccaaa tccattgcaa gagtcttccc acctttgccc ccacctaata tgatggtatc
    12961 aaactctaaa accattccat tctcccgata atttcaatgt tttatccact tgatgatgat
    13021 ccttatttac aatacttgga ttaaagagga aagctcacgc aaggcgataa aaaatatggt
    13081 gtaatctatg ttttcgctac ccaataacat ttctaataac ttttcccagc gtttttgaat
    13141 ggggtgattt tgttccagcc aatagtttat caatttttca gaagtacgtt ctttggtgtt
    13201 agttgttaaa atggcaatag tcaaacgtct ttgaagatta tctaactcat cacgcaaaga
    13261 caggcgagcc atggtattcc aatgcccttc acgtgagtca ttggctattt ggtcgcgaaa
    13321 ccaaacgaga ttaaattgac tgccaacttt aaagtaagtt tctgcagtaa gcgataaatc
    13381 aaatttattt tgcatggcta cttcaatgat atttaatgaa gtataaagag cccgagttat
    13441 agcaattttt tgagccatat ctttttcaat gccaatactg acaaattggc taatgagttt
    13501 ctcaaggtat tctttagtga caccacccat gagcatggga atcaactgtt caagtttttt
    13561 gactgacttg ccataatttt taatattttt ttcaatacct ccggtcagtc ggttattatg
    13621 caaaaaccag cgtgtcgcca aattgagcaa ttggcgaata tgatgcaata attcatattg
    13681 ggtggataag gatattttat cttgaaatga atcaatcaga ttttgtaaga catccgtttg
    13741 gaaaatacag gctgaaatag tgtaagcgca ggcaatatca gcaatcgaca taccggtttc
    13801 catcagcatg cggtacatga aggtaatgcc catgttgttg accagcttat tgctcagttg
    13861 cgtcgctatg atttcacgag acaaagggtg ttttggcaag gctttcgcat agggctttct
    13921 tagcgagatg ggaaaacctg tttccaaaaa agttgtaaag tatgagtcat cgggtaaatg
    13981 tgattttaat aattcgttag caatatgaat tttggtgtgt gccattaaaa cagcaagctc
    14041 tggtcttgtc aggcctgcgc cagtggtttt gcgttcgagg agttttttat cgtcaggaag
    14101 gaattcaacg cttctgtcaa ggttaaccca cttttccaat tctttcatgt aatcttgata
    14161 taagccacta taatgagaag aatgagctgc cgatatactt aaaatcaagg cttgattgta
    14221 gttgtcttgc aaaacaagat cggcgacttc gtctgtcatt ctgcttagca attgattgcg
    14281 ttttttttct gtgagtttct tttctctaat ctctttatct aaaagaattt tgatattgac
    14341 ttcatggtca gagcagttaa caccggcgga attatcaatc gcatcagtgt taataagacc
    14401 gccctttaat gcaaactcga cgcgtccaag ttgagtaaaa cccagatttc ctccttctcc
    14461 cactaccttg cagtgtagtt ctgcgccatt aatacggcag aattcattag tcttgtcgcc
    14521 aacatcggca tgtgactccg tgcttgcttt gacataggtt ccaataccgc cgttaaatag
    14581 taaatccact ggagctttta acagcgctcg aataagctcg ttgggcgtaa gggcatttgc
    14641 ttctatagcg agagcttttt ttacttgcgg gctaatgggg atcgatttgc ttgagcgttt
    14701 gtaaacgcct ccgcctttag aaataagttg taagttgtaa tcttcccagg atgaagtagg
    14761 taaattaaac aggcgaagtc tttcttcata ggattttaca gggtcgggat tcgggtcgag
    14821 aaaaatatga cgatgatcaa aagcagctaa cagccgtata tgttttgaat aaagcatgcc
    14881 attgccaaat acatcaccac tcatatcacc aatacctact accgtaaagt cttgttgctg
    14941 aatattgatc tcaagttcac gaaaatgtct tttgacagat tcccacgcac cacgtgctgt
    15001 aatacccatt tttttatgat cgtacccggc agaacctcca gaagcaaaag catcacctag
    15061 ccaaaatcca tgttccttgg caatactatt ggcaatatcg gaaaaagtag ctgttccctt
    15121 gtcagctgca acaaccagat aagggtcagg gtcgtcgtaa catataacat tctgtggagg
    15181 gattgtttta tcattgatta aattatcggt gagatccagt aaaccattga tgaacgattt
    15241 ataacaagag atgacttcct gttggatttg ttcgcggctg gctgcatgaa gcggtctttt
    15301 caagacaaaa cctcccttgg caccagaggg aacaatgact gcgtttttta ccttctgagc
    15361 tttcataagt cctaaaatct cggttcgaaa atcctctggc cgatcggacc agcgaatgcc
    15421 gcctcgggct actttggtac tacgtaaatg gattccttca aaacgtgttg agtacacaaa
    15481 aatttcataa agcggttgac ctggaggtaa gtcaggaatt tccgaggatt ttaatttgaa
    15541 ggaaaggtaa tctttagata atcccgcgtg atttttttga aagtaattag tgcgaagagt
    15601 tgctttcatc aaagaccaaa aataacgcat gatgcgatcc tcatccaggc tgctaatcgc
    15661 atcaatgttt atttgaattt tctgttcgag tgccgacatg tctttttgaa tagcagattt
    15721 ccttttaagg ccaaatttca aataaaaaaa gtgaatcaga tcacgggcaa tggctgcatg
    15781 catttcgatg gttttttcaa tgtaagattg actgtaacga atgccaatct gatgcaggta
    15841 ttttgcatag gctcgcaaaa tagtaatttc ttgccaggag agccccgcgg ataacaccag
    15901 tttattaaaa ccatcgtttt cacaaatacc aagattgata ttaatcagtg cctcggcaaa
    15961 aatattgttg acctgggtaa tattgaatga atcagtttga gaataggtta cgttaaaatc
    16021 actaatccag aataatcgcc catcccgtgt tttgatttta taaggacgtt cggtataagt
    16081 gcgcagcccc atgttttcaa gcataggcaa gatgtccgat agggggattg gagtctcata
    16141 acgataaaat ttaatgtgta aaggatattg taggtgagtt ttttcataaa aatcgataac
    16201 caggggatta tctttggaca gtttttctat ctgtaaaata tcctggattg cctcttcggg
    16261 agtatgttgc tcacaataac tcattgacat tgtttcggtg tatttatcaa tgagctgttt
    16321 cccttttggg gagccaaatt cctgagttaa ttgcttttgc aatttatctt tccatgagtt
    16381 tgtcatcatt cttaaaattc catgcaagat atgtttttat cgttactcaa aagtataggc
    16441 catttcaaag aataaaatta aaatttgcta acttatctcg cacaaaatga atcatttgtt
    16501 ttcaggaaat tattaagtaa gctttaataa aatgttaagg acataaaagt ataatcaagc
    16561 ttcttgcaaa agacgaagca tctattatgt ataaaaatat actgtttttt tcaaatttta
    16621 gccgtatgcg ttggaacgag tacgacgggg atcgatatca aagtctttgg gaacataata
    16681 agcgagatat taaacacaca tttttcggca cgattgcaaa cagaaaagat ttgcgagaag
    16741 tgctattcca gccagttact gcttcttttt cgatgctagt cgcgggggca tttgcacaat
    16801 tactggtgcc ctattccatt ttcatgctct gtatgggtga ggcggagttt gcgggcagaa
    16861 cattaattaa ttatcttgaa ataactactg ttgctttggc aatgccattt attgctttat
    16921 taagtactgt aggccgatgt attgcaacgg ttaaatgcct ggttgaagat caatacaatt
    16981 tggctgagat ggttgaagac aaagaggtta agccaaataa aatctatctt gattgtgctg
    17041 aagtaaacta cactgtcagg gatgagaaca acaatcagtt cagccgcaaa tattataaat
    17101 tccaatataa aatgagaggt ctcaacgatg agctttgcaa gggaagcttt actttcagag
    17161 atttagagcg tgttcctgat aatgacgatt tcaatggaaa gctttccagt ctcacacttt
    17221 ataaatacga catcctggat ttgattaaaa gagttcataa agataaattt gaagaaatgg
    17281 aagacgaaaa tctaattcgt atatccattt aatttagatt ggttagagaa tcaggctgtt
    17341 ttgggctgga agctgtaatg gagagtgatt atttttctca actacttgcc acttaagaag
    17401 ccacgttata tcgtttatga atgatatgat tggttcctgc ctatattctt gatgcgctga
    17461 cttggtaatg agttattgag ttggcgtcta aagcaagaca aaacataggg atgaaataaa
    17521 taaaaggatt aagggattaa gagcgtgcgt ttatcttatg gtttattagg tttcatgata
    17581 tttaatttgg tggcttgtga aaaaatggca ttgctgacta cgccacctaa aaagcagcaa
    17641 ttatctaata gtccactggc tactcgggca gagcgatatt tttggcgaac ccttcatgag
    17701 gggcgatatc aggacattcc ccaagcggat tatttgctga tggcagctta cctcgagaat
    17761 ccgtatgatt cgaaattagc ggcccatctt ggctttattc atatttggaa aattacggag
    17821 cgggaaagag taaaaaataa ctctcccttg cttcccaatg aaattatttt gtctaaaaaa
    17881 tactttgctg atgctctgca actggagccg gaaaacccaa tttatcaagg attttatggc
    17941 gatacccaat tagtagaagg gcagattttt aaagacaaac aagaagaagt cagaggatat
    18001 tttactttaa agaaagcgat aagcaattgg cctgagttta attattttac tgcagggtac
    18061 cccatgagta gtttaagtgc tgattcggat cattttaaaa agggtttgga ttggcaatgg
    18121 aaaactttgg atatctgtgc tggtgagaag attagtagac agaatcctga ttttacgcct
    18181 tatatgaaga gggaaacgac aataggcgcg caaagagcct gttggaattc agaaattgcg
    18241 cctcataatt ttgaaggctt ttttatgaat atgggggata tgttagttaa atccggagat
    18301 cctgaaacgg gaataaaaat atacaataat gccaaattga ctaaaaatta caacaaatgg
    18361 ccgtataaag atatgttgga gaaacgtatt ctgaatgcga aagtcaatgt acaacatttt
    18421 aatcaaaaat caaatgaccc caatcagtcg attatgttta attcgggata tggatgcgtg
    18481 gtttgtcatc aacggtaagt accatctgat tattctctat caggtggctt attagattag
    18541 taagaatgtg aggtttcagt ttaaaacctc tgcttgtgtt tttgcaaagc gggcaggata
    18601 aaagcgaaag aaagtgttat agttttaatt tatttcggca aagaatttaa aaagaatatg
    18661 gaaaaaatta ctatttatca taatccacga tgctcaaaat ctagacaagc attggaaata
    18721 ctgcaaaaca aaggttttga acccattatc attgaatatc ttaaaactcc tcttgttctg
    18781 gaacaattga aaagattacg gactcacctg gctcttgaag acatggtgcg cacgaatgaa
    18841 aatgttttta aagaattggg tctgtctctg gctaatgagg cgcaagtttt agaagctatg
    18901 gtaaaagaac ctatattaat gcaacgccca attgtcacat ttaaaggtaa ggctgctatc
    18961 gggcgcccgc cagagaaaat cctggagctg tttgattagt ctttattgta cgataatata
    19021 aattaatcct gataccatga ctaatctcac tgtcatggta aggaataaaa gcatttgcaa
    19081 aacttcagag agaatagctt tttaattgtg atgcaaattg ccttaacaca agcagtccgg
    19141 actcttcggc ctggctacac catttttgta ttaaattaag aagatcctta ttggataggg
    19201 acgtttttag ccatatctgc tgtaataatt cgcggtaatt atgaaccgct tgcaagttgt
    19261 catgttgctt gagtaacgtt ttcaatcgca actgatcagc atgggatagc tgttcattcg
    19321 gtttcttgag taaatggcga gcattcttta aatcccgatt ttttatgcgg tattgcatat
    19381 cgcgcagagt aggaaatata accagcttgt aataatcaga tatcacttga aagcgattgc
    19441 caagtatggc ttttaccgaa tctacatcta tatgagattt attcttgtca atgatcagtt
    19501 ttgggggtaa tttttttact ttggcgagtt ttaaaaaaga caaacaacgt atgtaaaacc
    19561 agcccaaatc aaattcccac catttaatgg aaaatttggc tgaggaggcg aaggtatgat
    19621 gattattatg caattcttca ccagcaatca ataaacccca gggaaaaaca ttggttgaag
    19681 aatctgacga ttcatgattt cgataacccc aataatgccc tattccattg accacgccag
    19741 caacaagagg tatccacatc atttggattg cccaaagggt aagtccagga attccaaaca
    19801 agaaaagatt aagtgcgagc attaaaaata tgcctttgtt cgcatgggga gagtataatt
    19861 tgcgctctat ccagtcgttg ggggtaccat aggagtattt tttaatcatg tctttatctt
    19921 tagaggcttc tcggtataac tctggaacct gccaaaatac ttttcttata ttaaatacct
    19981 gaggactatg aggatcccct tccatgtcag tattggcatg atgtttccga tgaatcgcta
    20041 cccattccgc agtggtcatt ccggtcgtta accaaagcca gaaacggaaa aaatggctga
    20101 caagaggatg cagagttaat gcgcggtgtg tctggtagcg atgcaggtaa atggtaacag
    20161 cagtggtggt gatgtgagtc aggacaaaac cggtcaaaag atatccccat aaagtcatat
    20221 ttaaaaaacc aaacatcatt tctcctttta ggtgcctaaa attgtgtaat tggcaaaacg
    20281 atatgtaaga atatctctat atccactaag tatatactat ttatttttat cttagtggca
    20341 tttaaaaagc taattattta cgcgctttta ttaaaagccg tcctggattt tctgctgata
    20401 ttgcccttat atcccgcttt tggcggcgca aatttttgaa ttttggctga gactaaatct
    20461 gaaaagttat gcaaaaactc aaaatcctta attttaatgc gctccagttt ttgattgatt
    20521 aatttttcta tagacagcag cagcttgatt tcttctgtgc tgaccaagga tacagccaat
    20581 ccagaggcgc ctgcccgtcc tgttcgtcca atccgatgaa cgtagtcctc aggcacatga
    20641 ggcaggtcaa aattcactac gcaggcaagt ttctcgatgt ctatgccacg tgctgcgatg
    20701 tctgttgcaa ttaatgtttg tactttgcct gacttgaaat cagccaaggc ttttgttcgt
    20761 tgagcctgtg atttgttgcc atgaatagca accgaatata tttgggattc ggccagttgc
    20821 ttaaccagtt tattggctcc atgtttggtt cgagaaaaaa ccaaagtttg tccccagtta
    20881 ttcttatgaa gtaaatgact cagaagggct aacttatggt ttctatctac tgaataaaca
    20941 gtttgtttta tggtttttac cgcggtattt ctcggtgcga tatcaatttc aaccgcttta
    21001 ttcagtatat ttctggctaa agtacgtacc tcaggagtaa aggttgctga aaaaagcagg
    21061 ttctgccttt ttaaaggtaa taatttgata atttttttta tatcatgaat aaaccccata
    21121 tccagcattc tatccgcctc atcgagtacc agggtgtcaa tctcatcaaa tttaacagca
    21181 ccttgctggt ataaatccaa taatcttccc ggggttgcaa caagtatctc aaggcctgag
    21241 tctaattgtt taatttggtt gtggcttttt actcctccgt aaatcacagc cgagcgcagt
    21301 gttaggtatt tgccatattg gattatgctc tcgtggacct ggatggctaa ttctcgtgtg
    21361 ggcgttaaaa tcagtacttt aactcgatta cggtttgttt gagtttgctg actggctttt
    21421 tgcaagatag gcaatacaaa gcttgctgtt tttcctgttc ctgtttgagc ggaaccaagt
    21481 aaatcatgcc catttaaaat gagcgggatg gcttttaatt gaatactggt agggttagtg
    21541 tagcctaact cactaaccgc cctgtttagc ggctcaatca gcgctaattg tttaaaactc
    21601 attgttttcc ttattgatat ctattggtat tgtgggctct gagtttaatc actcattatt
    21661 ttgtggggaa aatgaggcat taattagagg ggaaaaatag gaattttcaa aggttttttg
    21721 aaagaattca tgcaatattt ggagctatta taacagttat ttgcaaatat gtcttgttta
    21781 ttatgcttag gcttggattg ttaattaaca ggtaacatgc taagctcctc agtagattta
    21841 attctacgca caatactctt ttcgatcgtt tgatttttta ctcttcttgc tgtcctccag
    21901 atcattgttc gttaatctat tcaggagcaa tgatgaaaca aaaaaaaata tatgttggta
    21961 atttaccatt tggtacgact gaagaagcat taagcattca attttctcaa tatggaaaaa
    22021 tcgaggaaat gttcttaatt aaggatcgat taacagggca aatgaaggga tttggtttta
    22081 taacgttttc tgctcagcag gaagcggaat ctgcgttaga gatgaatggt cagccatttg
    22141 atggacgttc attaaaagta tgcatggcac aaaaaaaaac taattctgtt tctcgtagcc
    22201 ggtggtaaag tagagtattt acataattcc gatgttatcg tatcaagaac tgcatcactg
    22261 cagataaatt tttaatctac gttggctcaa ctttttcagc cccgagtgag ccatttattt
    22321 tcttgtgttt tatctatccc atatgcaaaa ataacaacca tagcatttgg tgataataat
    22381 cagcatgaaa gcgaaaggtt tttttgatga gcaatcttcc ctgcagaaaa gtaaaattgg
    22441 gttgaattta gtatggatgt tctcacttgg tcatattttt ctaataacgc tcagtaatgt
    22501 tctggttcaa tatccatttg caatgatggg atttcacacg acctgggggg cgttcactta
    22561 tccagcaatt tttattttaa ctgatttgac aacaaggata gcaaccgcta ataaggcgcg
    22621 gaaaattatt atgctgagta tgaccccagg attaatattg tcctatcttg ttgcgagcta
    22681 tattgaggca ggcaataatt tgaatgagaa cggtctatcc gttttacacc tcatgccatt
    22741 acgtatcgcc ctggcttgtt ttatagccta cgttcttggg caattgctgg atgtggcagt
    22801 tttccaacgc tatagaaatc ggcctgcctg gtggctggcc ccaactctat cggcggttgc
    22861 aggcaatttc atagatacgg cattattttt tactatcgcg ttttatcact gctccaatcc
    22921 atttttaagt cagaattggc ctgaaattgc gatggtcgat gtctttttta aaatcatgat
    22981 tagtttaatt gcttttgttc cggtttatgg tttggtttta agtattcttg gtataaaaac
    23041 taccaataaa ttaatggtat aaaggtatct tgacaccatt ttcaaaataa ggattgatga
    23101 tgctgactgg tttaaggctg cttacaaaag acctcaagat tttaaatggc aaatatattc
    23161 agctagaacc cattaccagc cagcaccgtg aggagttacg aaaagcagca aatcatgaac
    23221 aaatttggca atacatgccg caaaaggcaa cgggcaagtt atttaattcc tggtttgatg
    23281 attgtcttga aaaaatgtcc gcgagagacc aaattactta tgcggtgcgt tgtaaaaaag
    23341 aaggggatct gaaaggggcc acttcatttt acgatattca accagaaaac aaacggctgg
    23401 ctttaggcta tagttggtac attcctaaag tatggggaac aacgacaaac ccggagtcaa
    23461 agttgctcat gttgtatcag gcgtttgatg cctgggggat taatcgagta gaactgggta
    23521 cagattctcg caatattcat tcctatcgtg ctatcaaaaa gctgggggcg acggaggaag
    23581 gggtgttgcg ccaacatatg attcttcata atcaggtgat aacggatact attatgttta
    23641 gtattctttt ctcggagtgg ccagcggtaa aagagcgatt aatgcagaga ttgaactatg
    23701 ctgctgccga taaagaccac ttggctgtct cgatatagta atcccttgtt ctctgggtta
    23761 aacagctcaa ctcatgaaat aatcagacac acaataaata gaatgaaaaa agattggtat
    23821 ttttctttaa ttgagtgaat atttggttct acctgcgaga aaggagtgct caatgtacaa
    23881 caaaatgttt aagcctctgg atactgaccc gattttatat ttcaaaatgt acagtaatta
    23941 tactgaggga agggttgatg attgttgtgc atttatttta atgcccagtg ggttacaaag
    24001 agaatgggtc tgtttgcaat cgattcaatt tgcttttaat aagcgtggtg acgtccttgg
    24061 gattaacatt attttttctg gaaatgagtc gaatatccac aaaaaggttc gagaaacgat
    24121 ggaagggatg ctcaagctga agcttcaata tggaagaggg gaagagcttt tcgtttttga
    24181 tgaagaaaag aaaacatttc atatggggat agttcctggt aaggatacac aggcgtattt
    24241 ggagggcatt atcgctttta ttaaggatag ttatcgcttg caaccggact ttgcccaaga
    24301 catcaagtca caacttctta gtaaggaata tctggcacag gaatatagtc gtttgcgatg
    24361 gaagccacca gagaaagaaa ctgtttgtgt gcttatgtag gttgtagaag cattgtttcc
    24421 actttaaaaa tagaaaaatt tgaattttat ggggactatt gcattccatt agcttggttt
    24481 gcgtaatact ctcttgacaa ctacaagatt gaaagctcaa tattctttat ttacaccaag
    24541 gttcaggatt ttcattgtat aggaagcgat ggctcatttt attgtgtgct ttgttgaata
    24601 atggttgcct ctggcttggg tcagaataca ggaaacctca tgtcaacacc ccgcaaaaat
    24661 ggccgcataa atattcaatt caaactgagc aagctgccta tttacctgat ctgcattggt
    24721 ggaaacagtt caatagcaag gagctcaatg cttctatcca aaaggcatta caaaataatc
    24781 accagattca cctcgctatg gcaaatattg aatccgcaca ggggcaattg cggcaagtcc
    24841 agttaagttg gttgcctaat ctcacagggc tggcaggcta cacccagttt cccgttttag
    24901 gtaatccggg gactacggca attgcctatc cagcctacat cattaatatt ttccagcaat
    24961 ataagcaaca aaaaagcgcg caagccatgc ttgaagccag tatttacgcg caatattctg
    25021 ccagactggt cgtgatagcc cagactgcag ccagcttttt taccttggtt gctcagaatg
    25081 aagctttgca tttatacaat aaactgctta aggattatcg aacctatctc aaactgactc
    25141 aaagccaata tcgctcagga ttgatttcgc ttgataatat cactcaaatc aaaagtcata
    25201 tccaacgaat tgaggcacag atcaaggtgg ttcagcataa cattgtggtg actaaaaacg
    25261 ccttgcattt tttatttaat gaaaatcctg gagatgtcga gactaaaatt ctctttgaaa
    25321 atatcgatag taatcaattg gtaccaggga atttacctgc cagcgttctc agagtgagac
    25381 ctgatattca tgaggcagaa gcgttgttaa aagcagcgca tgccgatgtg ggtgcgatag
    25441 ccgcgaatct tttaccaggg attaatttgg gcgcttacct gggtgaggga tctaatgtgg
    25501 atggcgccat taaattaggg caagcttatt taaacggccc gataattgat ttgcctctat
    25561 ttgctcaggt tgatgtgagt aaagctcgat ataaggcaat ctatatcaaa tacattacta
    25621 ctattcgtaa ggccttgcgt gatgttgcta atgacttgtc tgcctattca acttccagcc
    25681 agcaattaaa taataattta tcagctttaa gcgatgaaaa acagcaatgc catttggcag
    25741 aagttcgtta tcgtcacgga attgatgatt atttaaatct aataaaatgc caaatcctgt
    25801 tagacgaatt taagttggtg ataaaccaga acaagctgga aaaactgcta tccatagtcg
    25861 ctttatacca ggatttagga ggtggatacc gtgggcattg atcagcaaaa gaccgtccgt
    25921 accttgcgct tatccctgac gtgtattttt ctttttctca ttacctggta ctaccaagtc
    25981 ccagaaagcg catggacttt agtgaccata tggtttgtca tgtatgaata ttccaccgtt
    26041 ggtggcgtac tgacaaaaag ctttttgcgc ttcgcaggca cggctttgag tgccatctat
    26101 ggaatgatag ttgtttattt ttgtgccaat aacccgttga ttaacattat ggctttagta
    26161 cctggtttgt ttctttacgc ctattttttc atggggggag ataaaacgta tattggtacc
    26221 atcggggctg taacgcttac tattgtattg ctgaattata acgacataga tacggctgtg
    26281 ttgcgggtat tcaatgtcat tatcggcgtc attggttcta tgttcatgat ccggtttttt
    26341 tatccccaat atgcaagaga taaagtttta gagattcagt tgaactttat tactcaactt
    26401 gcaaatcttt tagaaagcta cctgaatcca acccagcctt tggcagctat ccagacaaag
    26461 tatttagatt atgaacgcaa gatgcttgat ggttttactt tatacaatcg ttatattggc
    26521 gaagccaaaa tagaaacaaa aaaagctccg tttttcatcc ctcattccat tgctgccatg
    26581 caacatttca ggcgtctgtt tcgtttgttc agtgtgtttg tttattattt atctactgag
    26641 gaaattcgat cggatccatg ggtatgtgat caattaagta aacttctgct taatttacag
    26701 tcactgcaac gaaaattgct gaaacaggat gcggggccca tagggggaga cgttgtggct
    26761 gcagagccag aagaagaaat cgttatagaa acacctaata ttgaaaataa aatagcaaca
    26821 gaagctattg tcaataatat gcagaaagaa attgctttgc ttgatgctga aattaaggaa
    26881 ataatattaa tttacgatgt ttatgatatc aggttgaatg agatgtaaag actcattcca
    26941 atatcttggg aagggttcaa tagaaatgag gatgaaaaag acacatgagt gacaaaccca
    27001 aagctggatt ccctgtaatt attttattat gcgtgtttct attcattctg atttatcatt
    27061 actcgtattt atttcctttt acaaacaatg catttgttgt tgcgaatgtt cgtccagttg
    27121 ctgccaatgt aaaaggttat atcaccaata tttatgttaa aaatgaacag gaagtaaaaa
    27181 aaggccaacc attatttacc gtattcaaag ccccctatga attggcctac aggaaagctg
    27241 tgagcgatgt tcaagaggcg aaagcccaat tattgttatt caataaacaa gttgagaaaa
    27301 cgaaatatct gctgcaagca caaaaagagc tttatgaaaa attccgcttt gattatgagc
    27361 ataatcattc tgccttgcgt gatcacgcag tttctaagct gaccgtcaat acgatgttaa
    27421 aagaaaaaaa tgcggcacta agcaaattaa aagcattgga aaaagagttg gaggtcaacc
    27481 agcaacaagt cattgtgcaa aaaaagaaaa ttgactcttt aattgcggtg atgaagaatg
    27541 ccaaaatcga tttggatgaa accacagttt atgccaaaca aaatggggca gttcaggata
    27601 tgtttgttgc attgggaacc cccatcaaaa cccgaaaacc agtctttgcc atagcggata
    27661 ccgatacttt gtttatccag gctaatttca acgaaacgga tttaagaagg gtacagccag
    27721 gggataaagt ctctattttc ccgagaatgt attttggatc taaaatttat catggagtga
    27781 ttctttccag aaactgggct gcaagtcgcc tggaaaccca tcgggcaacg caaatacaaa
    27841 ttgttcgcaa tagcgaaagc aactggtttt tattgcccca acgcctgcca gtacaaattc
    27901 aaataacaga ctatgacccg gtgcattacc cgttaagtat aggcgctagc gcctatgtgt
    27961 atatccatac tcattaaaat tatggaaggt gttgaaatca agagtgcaat atattctgtt
    28021 tttttctatt tccagggtta atttttagct ggtaatttga ctgtgagcat tttttagact
    28081 aaccttacct taataaattg aacaaaaatt gatcaaaatg tgaatttcat agtttaatga
    28141 caaggaggtc atatgaaaat tccatttgaa gggaaatggc gtgcgaccat taccaaacgt
    28201 gaggctggtt ttagtcaacg tgtcgtggtt ggaggcgcaa ccagcgggaa tggcagttac
    28261 aatggtgttg ttggcaacag ctttgttttc gaagacggtt gggttgaact ccagtggaat
    28321 aacaacgcag gttcaggctg gcaggaaagc gctgtcatta gttctgtggg aatgacgagt
    28381 ccgctagttg ttgtaaaatt tgtttcagcc gatgacaatt ttccaaatca acgagacggc
    28441 gattatgatg atttgcaagt ccgttttgaa catcttgatg ctccttttga agtaacgcaa
    28501 agaccttacg cttttgatcg aggctccctt gtcatgtttc ctgacggcat tttcgatgcg
    28561 agccagggga ttcaatacat gggagtacgc atcaaaaaca cctggttttt tgattggcaa
    28621 agtaattttc cggcaacagg aatgaaaatt ggtattgcgc cagtgagtcg ttccgccttg
    28681 tctgctgctg gtattgtggt gattgatagt tggacgccac aggaacaaga aggtttgggg
    28741 caaatcatgg atggtggctt tgtgagagtt cctgatctaa aaattaatga ggaaacaaca
    28801 atttacttca agctggatgt cagtaatgcc aatccctcca aacctgaaat cggttttgtg
    28861 gcacagcgcg atgcttatga tccacgctat gatgagccaa cacgaatagt tgataaaaaa
    28921 ttttcatcag tcgctccagt tacaatcctg caaccaggga attggtgact gcgatccctg
    28981 aaggtacttt gtacctgaaa cttaataaaa ttgccattga tcgcaaaggc gctacaaaag
    29041 cagctcagga attgaagaaa tgccttaaac agcatggagg tggcataaaa gggatgtcaa
    29101 aatggctgca taagctaagc cgtggttttt ggcaaaatag taaagacaag gaacgttggt
    29161 gcaaggagca aggcaatgat gcattacgta agcttttaaa acagctttta gctggaaagg
    29221 acattgatat ttgtcatctt aaggctattt tggatcaatg ttgtgaacgc ccaggcggag
    29281 attgcgagtg ttgtccttgt ggtgatggag atggcaggcc gggtggttgg gatgatggta
    29341 ccggggttga tggctggtgc cgagtgaaac ctgtattctg gttaccgctg gattttgaat
    29401 ataaggtagt ccctaacccg gcttacattg gccagtttgg tcctcttgct ttcgaggatc
    29461 cctggtggaa agtagtttta atcatcttgg caattatact ggctatcgct tcagtcattt
    29521 atgattatgt ttatgcatca gaagatgacc aatacattat tgggcaggtt gtccgtaatg
    29581 gggatgctcc aatcaatctg gttgatgcag cagtgagtaa tcttaatggc tctcgtggag
    29641 tggatctggg acaactggat gcccagggta atgatattaa caacggcagc cccattactg
    29701 cattagacag tattattcag ctcgatcgta cagcagccgg aagttatttt ggtacgcttg
    29761 acgctgtttt gggtaatgtc gtttggaagt caggggctac cagtgcaaca acgagaggat
    29821 ttatcagtac tctgggaact tccgtcagca taccctatga tgagcatgat accctttcag
    29881 gtactgttac ttatactaac gaagtagtta tcagcgaact tgctgcgatt ccgcaaacac
    29941 tctcagatcc tggtgactcg ggatccgtat gggttgatct ggcaacagcg cgtccagttg
    30001 ctttaaatta cggcagccca acagactcca gtgataatcg ggcttttgct aatccgatac
    30061 gctctgttat tgacgtgctt aacttacggt tttaatgaaa aggaagtaag aaaatgaaaa
    30121 gggaattttt tcaaaaactc gacaagattc ttgatattgc ttccgaacat aagcaaatgt
    30181 tgttgcaata tcctgatgta gtcatggtag gtgttggacc tgagcgtcgc aagggaaaaa
    30241 ttacagggga gcctgcgatc attgttacgg tacgtaaaaa gcgctcatcc aatgaattaa
    30301 agaaacgggg ggaaaagccc ttgcctcaga caattaaggg aattcctgtt gatgtgattg
    30361 agcttggcaa agccgtggaa gctcccgaaa ttatcgctgt tcaggaaaaa gcaaaaaaaa
    30421 caattagtaa aattcgggca aaatggttac aaacaccaaa tattacagca atagggtatg
    30481 gctacaaaga gaaaggaggg gaaatcgatt ttaacaccat tgccatccag ttttttgtca
    30541 aaaagaaatt tcctatggtt gagctgcaaa agcagcaaat aacccccatt cctgaaaaaa
    30601 ttaatggggt gagtactgac gttattcagt tatctcccat ctgcccggct gcggcttccg
    30661 gttccagagg agatcgccat gatccgctca aaggaggtgt tgcaattgga gtaggaagca
    30721 agccatttca ttacggtact tatggtgcca cagtatttga tagaaccact ggcgaacaac
    30781 tctttcttag caatcaacat gttcttgatg cctctcctgg aacagacacc atacagcctt
    30841 cgccagtctt attggatgat tcagtggaag tagggtttca attggatgtt tgcttaccta
    30901 tccattttat caggctggat actccaaata ctactgttgg cactgtgttg gctggggctg
    30961 ccgtagctgc agctgttgcc gctgctttaa gtgatgaaat tgatccgaca aggcgaggtc
    31021 aggaagcaac aatgcctgcg acaggatcta aaacattatt tgaaacccaa aaagttaaaa
    31081 tggattatcc tgaattgccc attccaggga caccgtttaa agtaaagaca gcttgggatt
    31141 atgaacgcca caccgatgcg ggtattaaga cgttcagtgt cagtgaagaa aaaaagaatc
    31201 cccatgtttt acgcgataaa ttattaatta cagacaaaaa gctttatcac cctggggata
    31261 ctattcgtat ttttggttta atcttgccgg aggaatgtgc tcctgcatca cagtccagga
    31321 acgatcgtca acccattaat ccccgggaat tggaagatat aacccattat cctaatgtgg
    31381 cttcaagtat caatcttgat ctggtagcct caatggattc tgacattgcc tcttcgtcag
    31441 cgcgtcgcac gaatacaagg gctgctgtta ttaaccccaa acggtgccgt tgtgatcgtt
    31501 atcaccctgt cgccattctt acaccaacga cggaggatcg tgcttttcca gttgttttgc
    31561 gtgagccagt ctttgcgcaa aaaaaccaga ttttccaaga tttgctaacc gtagtgaagc
    31621 aacgagatga tgaaaaattg ctggagcggg tgtttgttct ggttcgttat ggttgtatat
    31681 ataccggcac attacgtgta aataacattc cagcgggtcc atggaaacat tttttctatg
    31741 ttcaaacagt taatatcgca acaagtgata tgaagccgct gctggcagcg caaattattg
    31801 gcggcttacc tgtttcccaa aatgctaaag cggacctgga tatagcctgt ggaccactca
    31861 cttgggaaga tggtgagttt gatattgagt tgatatagag tgtactgcat ggctgattca
    31921 tattggccca tagccatgga ataatgtgag ttgtattgat aagtaatgcc ttaaagtcaa
    31981 attattttca tcagctataa aatagtgcta tgcttttgcg gccaaaatgg tacgaagact
    32041 attaagtcac attagatacg ttttattttg aaaggaatac tctatgtttg gtttttttag
    32101 tgattacaaa caatacatca ctctgagaaa ttttgctgtc gtttataacg ggcttacagg
    32161 cttggcagta ttgtatagct tgtggagtaa tcctgaggct gatccctctg aatacgtcat
    32221 tgatatctct attcacgcgc tgactgctat cactttaatg tgtaaacaag caccagagtc
    32281 tgttaaagca gttgccatgg cattaaatac gtacagggga tttgatgctt tattcaaagc
    32341 cattacctca cttccttcca caataccagg cattgccaat gctgttgatg tattaaatca
    32401 ccggtttaat tttaaggaac ttgaaaaact cggcaatgaa gaaactgcag aaaccacatc
    32461 agcagttcag catacaatgt aaagtctgga tcgtcttttc cgatagaaga tggagttatc
    32521 agctattttc tatcgggttc tcttacgcca atttcatgct aaacagttta ttggccgtaa
    32581 tccaccagaa aatttttcac agctaaccaa tcaatataag ctttcttttt atccgagggg
    32641 ttacgcaaaa gataggcagg gtgatagctt acgataaaag gggtattgtc gtagtagtga
    32701 atatgattac gcagctgctt cagaggaagt ggtttatcta gtaagaattg tccggcgaaa
    32761 cgtcctaaag caaggattaa gtgcggttta atgagtttaa tttggcgggt caggaatgaa
    32821 ctgcattgag aaatttcctc aagcgctgga tcacggttat tgggcggacg gcatttcaaa
    32881 acattggcaa tgtagacgtc attctctgtc atttcaatac tacgtatcat cttgtttaat
    32941 aaaaaaccag ctttccccac aaaaggttgt cctttctgat cctcgtaaaa accaggcgct
    33001 tcaccgataa tcatcagttt tgcttttggg ttcccgcgag aaaaaacagt ttgagtgcgt
    33061 gttttatgga gggaacaacg tacacaggat gccacctcat ccgctaaagc cgctaaatgc
    33121 ttttcacaag aaggagtgga cttacgcatc acccagactt caatccccat agtctgcagg
    33181 tagtaattgt tcaatacatc tggcataaaa aactcatgat attaataaaa tccggattat
    33241 agattagact taccttttgt agcttggcac ttacgcaccc caaatctctt tgttaaaaac
    33301 attatttgcc tcggccttgg agttttgctt aagtcctata attaatctat tgtatcagtc
    33361 ctattttaaa gaaagaaact taaaaaaaca tcataaactc actgcctgta agaaatagaa
    33421 agttgagggg gtgaagaata ttgaacagag actggaaaga atatgaatag ccaaaaggat
    33481 ttgaaacaat tagatattaa gcaagtgatt gcctgggcat tctacgattt tgccaattct
    33541 tcctattttg tagtgatatt tacgtttgtc tttgcaacct atttcacaag ttacattgcc
    33601 cccaatacta tactgggaac cgaattgtgg gggtatacga tttcggtttc tgcgttactc
    33661 attgccctgg tgagtcctat cgttggtgca atagctgatt ttagtgggca tcataaatcc
    33721 tggctattcg tttttaccta tttgggtgtt atttcaacag gttgcttgtg gtttgcctat
    33781 cctgacagcc attctattcc cttggtttta acttgtattt tgattagcaa ttttgcattg
    33841 gaagtagcaa ctgtatttta taattccttt ttggcaaatt tagccccgga aaattatctg
    33901 gggcggattt ctggctgggc gtggggttgt ggttatctgg gaggattact gtgcctgatt
    33961 ctatccttat ttgtttttgt gaaaggtgaa cttggtggat tatggtttgg gacagagaat
    34021 tttgccaata ttcgtgctgt cactgtattt gtggctttgt ggattagtgt gttcagtttg
    34081 cctttgtttt tagtggtgca acagaaaaca ggagagcatt tgacagtagg tactgcgatt
    34141 aggaaaggtc tgcaagagtt gtggttaaca ataataagcc tgcctaaaca acgtgatctg
    34201 ttcttatttt taatagccag aattttttat atcgatgggt tgaattctat attggcacta
    34261 ggcggtattt atgcggctgg caactttcac ttgagtgtta gcgatattat ggtgtttgga
    34321 atcattttaa atattactgc aggtcttggc gccggattat tcgcctggtt tgatgattgg
    34381 attggatcta aaaaaactat tctaattgct ttagcgtgcc tgacactcac tttttgtttt
    34441 ctgttaacca tccactcaaa tacgttattt tgggtttttg caccggtgtt tggtattttt
    34501 gttggtcctg ttcaggctgc cagtcgtact ttcttggctc gtttggcaaa acccgaagaa
    34561 atgacgcgaa tgtatggttt ttactctttt tctggaaagg caaccagttt tttaggacct
    34621 ttcctggtta gcacaaccac tgctttagct gacagtcagc gtttggggat ggctgttctt
    34681 attccttttt ttatcatcgg aggcggacta ttattattgg ttcatgaaaa tgaataagag
    34741 aaattccggt aaggctgatt ttccctgctt tgaagttcag tatgagtttg aaagacaggg
    34801 gaaaaattcg cctctttgtt tttacaagtt atttcttatg ataaactttg ttctaatatg
    34861 aaaattatta tatctatatt atttgctttc attagcgcct ctgggcatgc gttttcttgc
    34921 tatgatccac agactatcat tcaaaaaccc atacagtttg ataaaaaacg gattgcctta
    34981 acccgggaat atcagcttac tcattatggc attgattccg agtcgataga aattgagcct
    35041 aaaatgattg tattgcattg gacttgtata ccaagcctcg atactacttt ccgcatattt
    35101 gatccccccg ctttacccac cagttcgcca agacgaaatg aattgcctgg ggatttaaat
    35161 gtatcaagcc attttttagt cgatcgggat ggaactattt atcaactgat gccagaaaca
    35221 tggatggcaa ggcatgtgat tggcttgaat cattacgcga ttggtattga gaatattggc
    35281 ggtgttgata gtaaagacga tttgactgat gagcaagtca aggcaaatgc ctttcttgtg
    35341 tgttatctca agaacaaata cccgcaaata aaatacctta ttggtcataa tgagtattta
    35401 cagtacaagg gaacggcttt gtggttggag aaggatccaa attatcaaac cgataaaaca
    35461 gatcctggcc caacgttcgt taaaaaagta aaacaattag tccttgcgtc ataagtaaaa
    35521 gtataggcgc ttatgcaggt aatgtatgtt ggagtttctg tttagctgtt aattgttact
    35581 tttgggttgt cctatagttt ttaaataaaa atcgggcttt gggtagtgtt ggtattcaaa
    35641 cgttgtaaac attcctgcat ttaaatgata tagattatgg cagtggttag cccagatgcc
    35701 agggtttagc gcatcaaact gtactttcac cgtcgaatgt ggttggacta aaagagtatc
    35761 gcgcatggct ccatccaatg gctgaccatc aatttcggtt acttgaaaga catggccatg
    35821 gaaatgcatg gggtgagaca tagaggattt attttcatag atcatttcaa cacgatcacc
    35881 gaaatggatt acttttggcg tgacatctgg ccagctttgc ttgttcatgg tccaaatata
    35941 accattcatc tgaccatcaa gcacatagtg caaacgagca tttatttttt tattggttaa
    36001 agcatttttt ccttttagtt ttttttccaa ctcatagtag ttgacacgtc ccgttttatt
    36061 agtggtattg ggagatagta ccggggtagg tgtgttatct gtttttagaa ttagaccggt
    36121 ttgtttattt gttccttctg ccatggcttt aacagggaat gctccgcctg cagaaggaat
    36181 ctcaacaagc aaatccatac gattaccaac gccaataggt aaagaactgt ctacaatcgg
    36241 ctcaatgttt tcaccatcaa cagcaatcaa agtggctgat agtttcccgg tatcaatttt
    36301 aaaattggtg gaggaagacg cattgatgat tcgtaacctt acctttttag agggttgaac
    36361 tgaaatcaca tcaggtgctg ccaatgtttt tttgttggcg agaaacgcat cataggtcac
    36421 gtcattcaaa tcgtttccag aatccatttt cttcatgtcc attttagcag ttcttaactg
    36481 atcaaatatt aactggggat ccttgtaagt aaaatcctct aaaaacagga tgacttcttg
    36541 taagtcttta tgcgggtcat ccttgtcata aataatcaaa ggagcagaaa gttgcttttg
    36601 ctcttgaaat ttttgatgag agtgcatcca ataggttccg gcttgcaata atctgtagtt
    36661 aaataattta ttttgccctg gtggaatcag gatttgactc acgtcaggta ctccatcctg
    36721 atcgcttggg acaattaatc catgccaatg aagagttatt ggtgacgcac ttttattttc
    36781 aactaaaaca tgaaacatat ctcctttatt ggcaaagtaa cctttgttgt cctttgtgcc
    36841 gtccgggtgg gtaaaggtga gtttaaactc ctttgttttt tttcctttac catcgacctg
    36901 gaattcctct tcactgacgg ttatgaccgt cggtttaagt ttgctatctg ttgcataaga
    36961 gaaattaaaa aaggttatca tgatagttat tgccatgata aaaacgaata ttttacccaa
    37021 agtatgtact ttaattttat tgattgacat tcttttccaa tgggctttgg cgagagggat
    37081 aattggataa aagtttatag tattggtcat ttcgctggtc tttccatgtc catgatattt
    37141 ctattatttt tatcagctaa tgaaacagta agcaaatctc tgatttacag attttccgaa
    37201 ttaatgttga aagtcaggta tgcgattgca aataattcgc atttgtatta ttgttctcat
    37261 ttaattaaat gacgctgtat tttcaagttg aaataattcg caaaggatga atgacttgat
    37321 gtttttacaa ggaaaataat gaaaaggatt tttttaatat cgataattgg gatagttttg
    37381 aattctgcat acgcttttga gcatattgga gaatgtgggg ctcatacctg gcatgaagtg
    37441 acatccaaat cgggttcctg gaaaatcact ttaaaaatga tcagtcagaa tggccagttt
    37501 gttgatcctt cgtgtgcaga aattattgaa accggattgg ctcagaataa ccctttgagc
    37561 tatggcctta aattcagcaa agacgctgat tttaatctgg cgtatacttt aacggctgtg
    37621 agagaggaac aggggtttca atcaaaaacc tgtgtgtttg ttatcactgc taatggtcca
    37681 gcgcaaccgg atattcaggc gcttagctat catggggctg aatgcacatg gcgggtaatc
    37741 aaaggggttg gggaaaattt tagtgtcggg tagcactatg aagcggccta aaaccacttc
    37801 ttttttctga aaattaaaaa taatagcaga gcacacagta cgatcaagcc aagtgcccaa
    37861 aagtagccat atttccagcc taattccggc atccaataga aattcatccc ataaattccg
    37921 gtaattaaac ttaaaggcaa gaaaatggct gcgaaggcag ttaatacttt catggtttca
    37981 ttcatgtttt gcatcaactg attgtcgatt tgtcctaata agccatttaa catatcgcga
    38041 agagagtcta tttcattgac aataaggtgg cagtgattcg ataaattata taaagaggtt
    38101 cgacattgct ctgatatgac taaaatattt cggctagtga tacgcattaa tatttctctt
    38161 attgctatgg tgtaacgctt cactttcatt acgccatgct ttaattccaa aacttgcgaa
    38221 tatgtgtttt tattgggggt gcgaacttgc gcgtccaatt gatcggccaa ttcttcatag
    38281 gccaaatgaa tcttggcata atcgttaatt accccttcca ggagtaaaaa taacaaaaaa
    38341 caggatgttt tagcataagg caaggatttt gggcagcttt ttaacatatc aaacaaaaca
    38401 gaagaaagtt ttttagaagc agtaaagcaa taattgggtg tgagatgaat aatcaaattg
    38461 ctaaaatcag catcataatt atcattcaac ttcattgaac acaagccttg aatttgcaat
    38521 gtgagtgctt catcgatctc tatggttgtg gatctgttgt ctttttctcc acacaattga
    38581 atcacttgct cgggtaattg taatttctct cgtaattttt caaagatatt tttttggttt
    38641 aaattgctgt gtatccaata aattttatct ttatcctgat aatcgatatt taaatcctca
    38701 atcgcaattt tttgcatact atgatttttt aaatcaaatt caacggcgat tgaatcaaac
    38761 gcatgcattt caatgctcat ccttattcga ttaatttcct catctagttt aaggatagga
    38821 gacttaaatg atattgacaa actctttttt atagcctggg ttttccaata gattgtgagt
    38881 gtctcgtgga gagctagcct cttgtttgat ttttgtcttg ttttaattct atccctatat
    38941 ataattcgga ttcttctttg gcaaggaaaa gtaaatttaa gccaaaagga acttaaatga
    39001 gtataaaatc agaatccatt aatacaggtt atttttgagc acttaatgag ccaagatatg
    39061 gaaaaaattg aaaagatgta tcaactgatt atggaaataa ataagttgaa gcttgtgttt
    39121 cgcaatacca taaccaatac gcaacggaat gaaagcacag cagagcattc ctggtcagcc
    39181 tctatgataa cccttattct catgaatgaa ttaaaaagag agtttaaagg catcgatgaa
    39241 ttaaaaatta ttaaattggt tcttatccat gatgtcgttg aaatttatgc gggagatgtt
    39301 ctggcttttg atctgaaagc aaggaaagat aaggaaaagg ttgaattgga agctcttgaa
    39361 aaactaagtg ctgtctatcc ttcctttggg atagaattgg acagtttatg gcatgaattt
    39421 gaggaaagaa aaagccttga ggcaaaaatt gctaaagcag ccgatgcgat ttgtccgatt
    39481 ttccaacgtc tccaggcaaa acaatcctat attccttttg atatttcaat ggctcaactt
    39541 gagaaaacaa aataccctta ttttcaattt agtaaaacgt ttagtaactt gtttgaaaaa
    39601 ttaaaagcag atttactcaa agagcaactt atttttttat agcgccattt atattcgctt
    39661 tcaacacatc ctgccagatg tcctgttaaa gagctcttaa gacagattaa aagttggtga
    39721 atcaaaacga atcctctata cttttaaaaa ctcttattat caagaagata agcattatgt
    39781 atgatgatct atggaagcct tatgcgtctg ccgaactacc ttctttaaaa ttgggcggca
    39841 ttgtcttaaa tggtcagtta ctctctgaaa cgccgattca cttagtgtta aaggctttca
    39901 atcgacatgg tttaattgcg ggagcaaccg gaacaggtaa aacaaaaacc atgcaggtat
    39961 tagctgagca attatcccta aatggagtgc ccagtttagt gatggacatc aagggggata
    40021 tttctggcct ggctatgcca ggtgaaactt ctaccaaatt acaggacaga ggtcagcagc
    40081 tgcatattga ttttactccc cgaggtttcc cggtagagtt attgacactg actccacaag
    40141 accccggtgt tccattacgt gcgagaattg atgattttgg tcccctgctt ttttcgctga
    40201 tgctggatct gaatgatact caggcaggag ttgtgactgt attatttgag tatgccaagg
    40261 cgaatcaact gaatttggtt aatttggatg attttaaatc tttgctccaa tttgctcaaa
    40321 cggatagagg caaccagctg attgccgccc aatatggcgg agttgcttcc acatccattg
    40381 ggactattgt ccgtaaaatc atcgagctcg agtctcaggg tgggaaacag ttttttggtg
    40441 aaccttcctt tgtcattcag gatttgctga gaacggatgc aaatggttat ggtgtcattt
    40501 ccatactaag attattaaac atgcaggaca aacctaaact attttccagt tttatgctga
    40561 agttattgtc tgatgtttat cgccaaatgc cggaattagg tgatccggac aaaccacgat
    40621 tggttctctt tatcgatgag gcgcatttaa tttttaatta ttccagcaaa gcattactta
    40681 atttaataga aacaatagtt aagctaattc gatccaaagg aatcgggttg atattttgca
    40741 ctcaaacgcc taacgatatc cctgaggctg ttttaagcca attggggtta aaaatccaac
    40801 actcattacg tgcttttact gcaaaagatc gaaaagcctt aaagttggta tcgcagaatt
    40861 ttccggtatc cagttactat aatactgagc aactactcac tactttaagt ataggggaag
    40921 ctttagtgag tgccttggat gccaagggtc aaccgacacc tttagttcat gcgatgatta
    40981 gagcacctga atcacgtatg gggcctttaa gcaatgagga gttaaaggtt ttactggatc
    41041 aatcgttcct ttttggcaaa tattctcaaa taatagacac gccttctgcg aaagagaaat
    41101 tggctgagct aatctctgag caacaagttg caaaacaaca gaccagtcag aaagaaaaca
    41161 attcctcggt gttgaatagt ttaagtaaaa ataccttatt ccgacaagtg gtacgtcaag
    41221 tttttaggga actcaccaga gcgattttgt ctttgttcaa gagtaaacgg aactaggcca
    41281 gttgttgtcc atattaaatt tttaacttat accgtgcatg gtcaaaataa aagcaatata
    41341 atcgacaagg atatacatga taaacatcac aggaacctac actgatcaat atcaattggc
    41401 tatggctcaa gtgtattttt taaaagggca tcatgagcgc atagcgacat tcgattattt
    41461 ttttcgtgct cttcctttta aaggaggcta tgcgattttt gcgggtttgg aggatttact
    41521 ggatgttctc gaacaacttc gctttgaaca acaagacatc gagtttttac aacatcagaa
    41581 catgcatcct ggttttattg actatttgca acagtttaag ttcactggag cagtgtatgc
    41641 gtcgcgcgag ggagatgttg tatttcctac gcggccagtg gttattatcg aagccaattt
    41701 gattgaagca caaatcatag aaacgttatt attaaatatt cttaattttc agacgcttat
    41761 tgcgacaaaa gccagacgca tcaggcaggt tgcgggggag cggattttaa ttgattttgg
    41821 actaaggaga gcacagggcg ctggagggta ttttgcaagc cgtgcagcca tagtcggtgg
    41881 ctttgattcg acaagcaatg tcagagccgg gcgtgattat tcaattcctg tatcgggtac
    41941 tatggctcat tcctttatcc aaagttacga tagtgagata caagcttttt ctgattatgc
    42001 tgaagcttgg ccggagaatt gcttcttgct agtagatact tataatacgt tgcaaagtgg
    42061 tgttcctaat gcaatcaaaa tagctaagga aatggagaag agaggacata ggctcaaagg
    42121 cattcgcctg gatagtggcg atttggcatg gttatccaag agggctcgcc acatgttaga
    42181 cgaggccggt ttaaactatg tggctattac agtttccaat caactcgatg agtatgtcat
    42241 caagaatttg ttagagcagg atgcgcctat tgatgctttt ggtgtgggga ccaatctcgc
    42301 gattggtgcc ccggatgcgg ctcttgatgg ggtttataaa ttagcattta taaatggtca
    42361 acctagcatt aaattatcag atacccctgc aaagatcact attccttatc aaaagcaagt
    42421 ctacagggtg ttcgaaagag atgggacgta tcgcggcgcg gatgttgtct gtctcaaaac
    42481 ggaaaaaaaa attgatatga tgcatcatcc ttttgaaatt ctaaaatcgc tttcgataaa
    42541 gcactataaa aaagaagcat tactgcataa agtgatggaa aacggcaaac ggttactgcc
    42601 taaaaaatca cttggagaaa tagtcagatt tagccagcaa tgttttgatc gattgccttt
    42661 ggaatacaag cgttttatta atcctcatat ctataaagtg ggtttaagta ccgagcttaa
    42721 aatgaagaga gatcaactga tagctgaaca ctcaagataa ttttatgaag actttaatca
    42781 tcctcgatgt tcaaaatgat ttcatgccag gcggctcact gggggtaccg aatggtgatg
    42841 cgattgttcc cgttattaac agcattttgc attgttttga tctgattgtt gcttcacagg
    42901 attggcatcc ttctaatcac attagtttcg cctccaatca tcctggtaag aaatcttttg
    42961 aaaaaatcaa gctggggaat ttagaacaaa ctttatggcc agatcattgt gttcagggta
    43021 gtattggagc gcaatttcat gcgcaattaa atactaatcc tattgaagct atttttcgaa
    43081 aaggaactga ccccaatata gatagctaca gtggttttta tgacaatttg cacaaaaaaa
    43141 ccacagggct tgccggttac ctgcgcgaaa aaggcgctag agaactttat ttttgtggtt
    43201 tgtgcgctga tatttgtgtt tattttacaa ttaaggatgc cctcagccag ggatttgatt
    43261 gctatttgat tgaggatgca actcaagcgc ttgtgaagga tgatttcata aaaataaaaa
    43321 aagaattgat aaaacaaggc actaacataa ttaacagttc aaatttgctt aataatttac
    43381 aataagaagg tctgattttt taaatttgca gctttgagct caccaattga tgatgaactc
    43441 gctgtcgaag gaaacccggc gtatttttaa gacccgctac cataaggttt cgtaatgatt
    43501 tcaagaaggt ggccattggg atcattaaaa tataatcctc gacccccatc attataattc
    43561 atttggccta atcgagtttg tcccggatca gcccaataat ccattttaaa ttcgcaaatt
    43621 cgtttaaaaa tatcatcaaa ttcagtttct gttactaaaa aggcataatg ttggggcgta
    43681 atttctttat ctgttttaca gaaatcaagg ctcacatggt tgctcatttc aacgaccata
    43741 aatgggccat atggaattgc ttcaggcaac cccaaaattt ctgtaaggaa gtgagctgat
    43801 ttgtcccgat cttttaccca tacgatagta tgattcaatt gtatactcat catgattcct
    43861 tgttcctgtc gatagaagaa attatttcat tacttttatg aaagaaaaga atagccacat
    43921 cattaaaata catagaatga tgtagatggc tggtaataaa tattgtgaca ttggaagtaa
    43981 gcttaatccc caaataacta ctgtaaccaa tcccatattg atgaagttca taaccgcgga
    44041 gccatgggct ttatcggagg tatggctcat ggcaaatgtc gaagcatttg caaaaataaa
    44101 agatagtccc aaatatatga ataccatggg aagaaatagc ttgataagcg ctgaatgatg
    44161 ggttaacgcg gccatcatca ttgccagtgc ggcaaaccct gagattaaaa tgcctaattg
    44221 gattcccgcg cgttgcgagt atttttttgc aaattgagca gaccagattg agccgagggt
    44281 cagacccaag gagggtaaaa tattggcagc gccatactct gctgtactca ttcctagcaa
    44341 attgatggca ataaatggtg ccgttgcggc gaaaatgtag acaaagcagg atgcgccacc
    44401 cattaatgct ccaccagaga ttaatggctt gtttttaaat tgacttacat atccctggag
    44461 cagatgtttc atttgaaatg cttcgacttg aagatttttt tgcgtttcag gaagttgtct
    44521 gaccatagca agcagaatag caccataaac agtacctgca taaaaacaac tttcccagcc
    44581 ataatgggag tttaaaatgc cgccaattgc aacactcaaa ccgggtgcta tcgcaaaagc
    44641 cagcatcaga taggaggttt tttgcgcggc taattggggc tcatagcatt cattaaccaa
    44701 agtaaaagtc atcttcagtc ctacacctga tcctaaggct aatagaaatc gtcctaaaac
    44761 aagcaacggg tacatggaaa tcattccggc caatacacat agaagactgc tgataatttg
    44821 caagccaata cctatataaa gtgttttttt tcttccatag cgattcgctg tagggccata
    44881 aacaagttgc cctatggcat aaccaatcaa aaaactggtg attgtctgct gtgcggcatg
    44941 ttctgtgatc ctgaaaaatg cagtaatgct gggcaatgcc ggtgtaaata ggacagcatt
    45001 cacagaagca aaagaaatta acaacaataa agtcatgaaa gaaagtttgg tttttggata
    45061 catgatgaga atagttttaa cgattaacaa ttaagatcag tataattatt gacaaaataa
    45121 ttaacaatat cataattaat taacatttat taactataat gcaataataa aatcgaaatc
    45181 atgggtaaat ttgatcaaat cacttacttt attgctgttg ttgaagagca tggttttgca
    45241 gcagccgcaa gaaaatttgg cgtatctaca gccgctgtta gcaggcagat tgcacgtctt
    45301 gaagccgagc taaaagcaga gctgttggtg agaacaacta gaaaattatc attaactgac
    45361 atcggggctc gctattatca gcaatgcaaa aagactcttt tggagttgaa tgaggcagaa
    45421 atagcgattg ctaatagtcg gaatgaagcg gtgggtatat taaatgttac tagcagccgt
    45481 tattttgcta tgagatttct tctcccttat ttaccagaat tcatggagtt aaatccaaaa
    45541 ttgcagatta aaattgaatt ggcggaaaga tttcctgatt tagctcagga aaacgttgat
    45601 gtgctttttg gtgtttctat ggagggcccc cctgaactgg tgcgcaagcg agtttctaca
    45661 acacggtatg tgctttgcgc atcgccggat tatctggcaa aaaatggtat tcccaaaaca
    45721 ccttccgaac ttgggaagca ccgttatatc actcacagca tgcgaaatcc tgataatttg
    45781 gttacattta aagacaatga gcagatttat gttgaacctg tgttatggct caatgatagt
    45841 cgtgccttgt gtgagtgcgc cttacttggg atgggaatta tcaaattaca tgaatacata
    45901 gtggctgaag cattgcaaga tgggcgcctc attgaggttt tgcctgaatt ccaagagtcc
    45961 agtttatcgg tgtaccttta ttatcaggca agccgatatt tacagcctaa gatacgaaga
    46021 tttattgatt tttttacaaa ataacaataa aagcaagtat tttgtgtcat tctagcagat
    46081 ctgagttcaa agcaaaaaag ttcgggggca cgtgcttttt ttagagagag atgtcaggtt
    46141 atgcaaggcc tcattatgcc gcaaattagg tgtatctgtt aataaatgct tcaggttgct
    46201 ttgttagaat aacaacagtc agaaacatta ttaactagag tatcttgaaa tgtttttta
//
LOCUS       DATNSN010000009        42641 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.009, whole genome shotgun sequence.
ACCESSION   DATNSN010000009 DATNSN010000000
VERSION     DATNSN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 42641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42641
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.009"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            67..558
                     /locus_tag="VAH76_000395"
     CDS             67..558
                     /locus_tag="VAH76_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385051.1"
                     /translation="MSKGPYQTFANRLISTLKEKGYSASRSPNGICIKTLAEFTGASE
                     QICRRYIRGDALPDYEKIKKLAYHLQVSPGWLLFGDDENTTYKKNEIDETLLHYILKQ
                     SHHLYPVSHGANDDYADFVLGLIKEVKTIDTSENNLLKIIDLAIGSISSYEENRKKHS
                     HGI"
     gene            548..1159
                     /locus_tag="VAH76_000396"
     CDS             548..1159
                     /locus_tag="VAH76_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgJ"
                     /protein_id="HEO1385052.1"
                     /translation="MEFKIFVVMDVTAVEVKLHPKAKEFLFEHFVTIRRVFSDVLGQV
                     ETDYASIALINQAGQIFFISSNPAIEQNLIEQSLWLFDGCYQPEFLSQDQPKLWSELP
                     HIGCTESIVKYKQIKPRLITGISIPTEYDSYKAIFSFGLKRINPYIQNKSSIHCEKLL
                     AMGKFALRRIQEYLIFPDKQPCMTTKPKLTLIINNQVPHEHTP"
     gene            1143..1661
                     /gene="def"
                     /locus_tag="VAH76_000397"
     CDS             1143..1661
                     /gene="def"
                     /locus_tag="VAH76_000397"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO1385053.1"
                     /translation="MNILLDKNDPILRQTADPISESEFGSSWLKELIKTMFGIMADKG
                     AVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTALINPSLKILSQEIQTGYEGCLN
                     CGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
                     SLTTLSAMQSKG"
     gene            1666..2958
                     /locus_tag="VAH76_000398"
     CDS             1666..2958
                     /locus_tag="VAH76_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385054.1"
                     /translation="MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGL
                     FLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAGSILTMGISTTLIGFIPPHSTIG
                     TFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
                     VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPK
                     FKSLLSESVTYIKNNKIQSLYVFILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIIS
                     NIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFNFLSHKIFYQLILSQ
                     ALIAVPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTG
                     VASSPSILVFTLIATMFIIIIYRTIKPEKRCSLTTLKG"
     gene            complement(3344..4372)
                     /locus_tag="VAH76_000399"
     CDS             complement(3344..4372)
                     /locus_tag="VAH76_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEO1385055.1"
                     /translation="MRLRLVILVLAAIIIGVGSYSGIVNVIKEPIENMIIGYKSPSAW
                     NNRGVDYVIGKRVAQNDSEAVKWFCKAAKQGEAMAQRNLGLMYAAGKGVPQDNGKAMQ
                     WFRKAALQNDAVSQLNLGVMYQKGMGTQQNDREAIKWIHKAAAQGFPEAERSLGILYS
                     TAENGQQNYVEAFKWLHKAAEKEDAIAQYNLAVMYVTGKGVRQNDTEAVKWFRKAGKH
                     GDLMAQRTLGLMYATGSNVQQDDFQAMKWFRLAAKQGDAVAQYNIGIGFLNGKGVIRN
                     HTKALKWFHLAASQGLPQAQYVLAALYHDGVSLPQNSMEAIKWLRKAAAQGHLQAQER
                     LQALVRQD"
     gene            5080..5289
                     /locus_tag="VAH76_000400"
     CDS             5080..5289
                     /locus_tag="VAH76_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO1385056.1"
                     /translation="MTNKIRGKVKWFNKDKGFGFIESSGKDYFVHFSSIQSDGFKTLP
                     DGAAVLFKMGKGQKGPQAEEVEVIK"
     gene            5374..6117
                     /gene="phbB"
                     /locus_tag="VAH76_000401"
     CDS             5374..6117
                     /gene="phbB"
                     /locus_tag="VAH76_000401"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEO1385057.1"
                     /translation="MNQRVALVTGGTGGIGTAICQEMQKLDYQVIACYFKQGNHDLAK
                     EWQKKQAMQGFDIDIVYADISSFNDCEKLTDLVIEKYGKIDILVNNAGVTHDATLKKM
                     TQEQWQDVINANLNSVFNMTKTVLSNMLDNSYGRIITISSVNGRKGQFGQCNYAATKS
                     ALYGFTKSLAQEVAKKGITVNTVSPGYINTEMLASLRDDILEAIVAQIPVGRLGKPQE
                     IARVVAFLAEEQSGFITGANFDINGGQYM"
     gene            complement(6268..8050)
                     /locus_tag="VAH76_000402"
                     /pseudo
     CDS             complement(6268..8050)
                     /locus_tag="VAH76_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(8228..9748)
                     /locus_tag="VAH76_000403"
     CDS             complement(8228..9748)
                     /locus_tag="VAH76_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1385058.1"
                     /translation="MIIVVWRLPLSDGNKALFERSYYRERKAREEAELLLENKTRELH
                     LLNQDLEKKNAELIKTLKDLERVQKKLSKLAHFDVLSRLPNRLQFELDLKREIARSKR
                     YGRHLALLSIDLDFFKNVNDRFGHDVGDGLLREVAKRLCSSLREEDCVARLGGDEFAV
                     ILTEINNPQQAKLVANNMIQKMGKPFHIKGHTVMIGISIGIAYFPDAGDEAITLRKNA
                     DIALYNAKECGRNNCQIFTPSLRKQYSKRLGIGTELHLALERQEFFLVYQPRVDLKTN
                     TMVGMEVLLRWQHPKRGVVFPDEFIAVAEHFNLIIPIGEWVLRTACRQYVDWKKRYAP
                     FNCTLAINISLHQFQHKGFISSVKHILQEYQMPSDWLELEITEATAVNFLGKIEETLS
                     ALRNMGIKLAFDNFGSGYSFLSHLKNSPIQSIKLKQALIEDAHLRENDNLIIKSTIAL
                     SQELGLNVVVGGVETEEQLNFLRSSHCFEVQGYYSSKPLTVVQMTQFIEERNSKGL"
     gene            complement(9714..10256)
                     /locus_tag="VAH76_000404"
     CDS             complement(9714..10256)
                     /locus_tag="VAH76_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEO1385059.1"
                     /translation="MKGIVFTSLNDMIIEQFGIETWDQLVSSLDLPSGGSYTAGGTYS
                     DTELQQLIQAIAKRTNQHASAFLESFGEYMFPILSSKYAIFLKKDMTLKEFLKSIDGT
                     IHVEVEKLYPDETLPTIHYEEPAANQLVMVYRSHRRLCHFAMGLIQGAAQHFKKRITI
                     NQTHCMLKKDDHCRLEITFE"
     gene            10524..10778
                     /locus_tag="VAH76_000405"
     CDS             10524..10778
                     /locus_tag="VAH76_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385060.1"
                     /translation="MKKQTDLIVLGKQIRKIRKEKGFSQEGFANFIEMNRGYYGTVER
                     GEANITILNLLKILKGLDVTPNELFPPSTYMSLKKKITRT"
     gene            complement(11560..11727)
                     /locus_tag="VAH76_000406"
     CDS             complement(11560..11727)
                     /locus_tag="VAH76_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385061.1"
                     /translation="MNNTGFKTIFFLIGVLLLGCSLIMSCTKTTATETHPESGYEGGF
                     GGDGGHRHQEH"
     gene            complement(11808..13949)
                     /gene="cadA"
                     /locus_tag="VAH76_000407"
     CDS             complement(11808..13949)
                     /gene="cadA"
                     /locus_tag="VAH76_000407"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEO1385062.1"
                     /translation="MTAPLTLTTFFVAGLDCQAEEQLIRRQLQAIPEVEKMDFNFIAE
                     EVTVHHRLPTIEPIQQQIEALGMSVRAKQSTPSDKVQEKLKFDASWQIIALSGLLALC
                     SEIAAYLLATEQSLWAIIPALLAIILSGPPTFKKGWLALRTKAMNINSLMLIAITGAI
                     FIGDWPEAAMVTVLFALAERIERYSLDKARLAIRSLMQIVPDVAQVKTANGQWQTLLV
                     EDVPLGAVFRVKPGERIPLDGEVVSGQSTVNQAPITGESMPVSKQIGDSVFAGSLNEH
                     GAFEAKVSKASGDTLLAKIGKAIEQAQAERAPTQRFVDEFAKYYTPIMVLIAILVALI
                     PPFVLGYPFYDWIYKALTLLVIACPCALVISTPVTVVSGLAASAKHGLLIKGGSYLET
                     GYRLQLIALDKTGTLTEGKPVVTDFITYDKSQTNEHLLLLAASLDSHSEHPVAHALVA
                     YWHQEHPDKSLLSVEQFSALPGRGVKGAIQGELYYVGNHQLAEDNKVCCLEVEQELKR
                     LEEEGKTTVILSNSTTVLAIFAVADTLRANSREAIESLHRQRIKTAMLTGDNALTAQA
                     IAKQVGIDEIQANILPTEKMQAINKLLEQYQAVGMVGDGINDAPALAKATISFAMGKG
                     TDTALETADVVLMNDNLAMLPLYIDLSRKTVRILWQNISLSLVIKTVFFILALGGMAT
                     LWMAVFADMGASLIVVMNGLRLLNSNQTKNH"
     gene            complement(14082..14378)
                     /locus_tag="VAH76_000408"
     CDS             complement(14082..14378)
                     /locus_tag="VAH76_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385063.1"
                     /translation="MLTQDQVIKLSDILHLMGEVNRLKLLIECLKGPKSVSDLAEQLQ
                     LSVPLTSHHLSLLRSARLLMANREGKHIYYSIYDAHVRCILEDMLKHFTEETEN"
     gene            14542..14742
                     /locus_tag="VAH76_000409"
     CDS             14542..14742
                     /locus_tag="VAH76_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385064.1"
                     /translation="MLDWASHNFLITAGIIFSIGLVLIVLAKGSSTHRMKLIANIGFY
                     LAFVALVVFVIVLFLDVLPRKP"
     gene            complement(14780..17938)
                     /locus_tag="VAH76_000410"
     CDS             complement(14780..17938)
                     /locus_tag="VAH76_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEO1385065.1"
                     /translation="MLEKIIRFSLKHRWFVLLFTAVIAALGVYNFQRLPIDAVPDITN
                     VQVQINTQASGYSPFEVEQRITFPIELAMSGLPSLDYTRSLSRYGLSQVTVVFKDGTN
                     IYFARQLINERLQEVKDKLPPEAETTLGPISTGLGEIFMYTVTNKTNVPASQRYNPTE
                     LRTIQDWIIKPQLRNVEGVAEVNTIGGYEKQFHITPDPMKLVRYRLSLNDIVEALERN
                     NANVGAGYIESNGEQHLIRVPGQVKNIADIENIVIASFEGTPVRIRDVAEVALGKELR
                     TGAATENSKEVVLGTVFMLMGENSRTVSERVAAKMKEINKTLPEGVEAVTVYNRTLLV
                     NATINTVKNNLLEGALLVCVILFLFLGNVRAALITAMVIPLSMLLTITGMVTNKISAN
                     LMSLGALDFGLIVDGAVIIVENCIKHLGEQQHALYRVLNLEERLKVIAYATTEVIRPS
                     IFGVFIITVVYLPILTLTGVEGKMFLPMAETVIIALLASMLFALTFVPAAVAIFLRGR
                     VQEKENWLVHYLSLGYARVLRRCFHARFLVISAAVVLVVVSLLVAFRLGGEFIPSLDE
                     GDIAMHAMRIPGTSLTQAITMQHLVEERVSQFPEVKRVFAKLGTAEVATDPMPPNVAD
                     TFIILKPRKDWPNPKKTKQELVQEMEQAVKQIPGNNYEFTQPIQMRFNELISGVRSDV
                     AVKVFGDNMDTLLQVAEAISAQLKKVPGAADVKVEQVSGLPLLTVAINRDVLARYGLQ
                     IGAVQDAIVIATGGKKGGELFEGDKRFDLVVRLPEALRSNPNVLRQVFIPLPPSKDNE
                     RHFIPLSEVATLVRSESPNQISRESGKRRVVVSANVRNRDLSSFVNEAKQRIEQNVKI
                     PTGYWVTWGGQFEQLQSASQRLQIVVPITLLGIFLLLFMSFGRVRDALLVFTGIPLAL
                     TGGVFALWFRGIPLSISAGVGFIALSGVAVLNGLVMITFINKLREQKQVYLKDAVLQG
                     SLARLRPVLMTALVASLGFVPMALATGTGSEVQRPLATVVIGGIISSTFLTLLVLPGL
                     YYVFHERRYRNKLPIKKAGEDT"
     gene            complement(17948..19201)
                     /locus_tag="VAH76_000411"
     CDS             complement(17948..19201)
                     /locus_tag="VAH76_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385066.1"
                     /translation="MTFLKPFKPSILLLILWFVMGVFSWAGEGSHAMEEGNHQEIMEK
                     GPQGGRLFKEGDVALELLIFERGMPPHFRGFVYEKGELIIPTKARLVVYLTRFNGKKE
                     TITFIPVENFLQSNEVIGEPHSFDVTIQLELKGKRLSWHYSTYEGRIKIAPAILKAAD
                     IQTSVAESQTIKTQLKVVGKIVPNRDTLAPIYPRYSGIIKALTKNLGDEVTKGEVLVT
                     IESNESLQNYDITAPITGTIVQKYATNGEQAQNTKPIYEVANLTTVWADFTLYRKETP
                     RVKQGMEVVVTGDEGKPKSTSVISYIAPLGIEDSQTTLARAVLSNEERLWLPGMYVNG
                     AITIKEKTVPVAVLLSAIQRLNDKDVIFVRQGDYFEATPVILGEKDEQWVEVVSGLDV
                     GQPYVSKNSFFMKAELGKEGASHEH"
     gene            complement(19198..20448)
                     /locus_tag="VAH76_000412"
     CDS             complement(19198..20448)
                     /locus_tag="VAH76_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1385067.1"
                     /translation="MRYFSRLVIFMMMYFVVCEVLSATPFTFKQALDTAYRNNPELQA
                     EMDKARAMRGYFIQSGLYPNPQLTLTAENFGGSGAYSSYESAETTFSVTQPIPLGHRL
                     QYLKKATYADYLASLASIRVQQTALYMAVGVAYVDALYAGQWHQVTKKLTRLNQDIVV
                     AIERRVKAGAGAELDLRLAQVRLGEAKIQENKAARDALLQRAKLARLLGDGLRVDRPL
                     VDKGLPGVSVNWTELVKKLPQSPQLLQMQLQLQAKRKTITAVKKSVWPDLNIQLGGRH
                     FSDDGSNAAVMSAFAEVPVYNRNQGKIMTAEAQYTQTAHEFQGTRLDVRQSVYAAFLQ
                     AQQNHYEASLVTDSLLPLARKAIKLAQEGYQMGRYTYIELSTALNSLYEEERHYQQAH
                     ADYHKALIQMTGLLGLDNSKEESK"
     gene            20831..21341
                     /locus_tag="VAH76_000413"
                     /pseudo
     CDS             20831..21341
                     /locus_tag="VAH76_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(21376..21549)
                     /locus_tag="VAH76_000414"
     CDS             complement(21376..21549)
                     /locus_tag="VAH76_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385068.1"
                     /translation="MKHFTLVKKSMEVLLVCLSFLLFSCSASVSKEDSKSNTPVKKRF
                     GGYHPHVHGRGTR"
     gene            21650..22261
                     /locus_tag="VAH76_000415"
     CDS             21650..22261
                     /locus_tag="VAH76_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO1385069.1"
                     /translation="MERLLIRFIEYNGFFTGIAKIYSTKKWSRKNLNHEILASSKHIE
                     HHCAHFNQGGATMKKRIIPSVCSGFILSFMTQIAIADDIPTRSLPMSQILQHLQSKGY
                     HTIQEVEFDDGLYEVEALDEQGNQIILRMNPQTGDLIKNQNTQKYPISLLEAAQKIEG
                     AGYHSIYKIESHGSQYEVKALGKEDTKVKLRVDANTGKINRDE"
     gene            22827..23288
                     /locus_tag="VAH76_000416"
     CDS             22827..23288
                     /locus_tag="VAH76_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEO1385070.1"
                     /translation="MKIVYLIISFMILLLNSGCGGLRQLNADAKNVNLYFSYPTGNCR
                     FLGIIKNPNVHEVMDIRSSLKDLEKDDNNFLKNEGAKLGANVIVLITHTPREAHKRYV
                     RGSKNLTTINIHSVIANAYYCPYVKTDQLQQRSLEIKQTPLFENDDLQINH"
     gene            complement(23496..23951)
                     /locus_tag="VAH76_000417"
                     /pseudo
     CDS             complement(23496..23951)
                     /locus_tag="VAH76_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
     gene            complement(24117..24371)
                     /locus_tag="VAH76_000418"
     CDS             complement(24117..24371)
                     /locus_tag="VAH76_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385071.1"
                     /translation="MNMKKIIGSRITQARKANGLTIKILAERTGFGAARIGNWEQGTR
                     SPGPEEALVLSKELVVAASWLLGISNDPHGEWMDHVIFEY"
     gene            24540..25439
                     /locus_tag="VAH76_000419"
     CDS             24540..25439
                     /locus_tag="VAH76_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385072.1"
                     /translation="MGFLFVTTNELSALMGLPYIQQSTYLLGIRPYMDRETFLVGVKR
                     KISYQQLAEALYIEPHQGFQQSGSPSRQQLRRIIYALERAGLIKIESIGMNLIVKCLL
                     ADTDISVQNKPDTNPTQQPDTNPNIKKNYKSSSYDDFSKKPNTVENIKPDTPHNSENL
                     CVYVRAQFQKFWELYPQKQDETRAFQEFYKLKPDETLFRQMMDALHAQIENRLDLELC
                     GEWVPKWKFPANWLAQQCWNDDLLPVRKQEQGHANTQTSFRKKSAADILAESCKESSF
                     SINFEDEPQTGNNVIHFDSTRSV"
     gene            25456..25809
                     /locus_tag="VAH76_000420"
     CDS             25456..25809
                     /locus_tag="VAH76_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385073.1"
                     /translation="MFLRFNAIYGALWLSAYNNEKALEAAKLEWADSIKEFDSQVLTF
                     AVEKIKRTQQRPPVIPVFVELCISIQKSIKAREEALRAKPENHKRTDPQIVKSHIKEM
                     MEKLTSPSVKEKKSC"
     gene            25803..26003
                     /locus_tag="VAH76_000421"
     CDS             25803..26003
                     /locus_tag="VAH76_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEO1385074.1"
                     /translation="MLILDRKIGEEIYINKGKIKITVLYEKNGLIGIGVRASSEIDID
                     RKEVFIRKYIQKLDQENKSNQG"
     gene            26012..26425
                     /locus_tag="VAH76_000422"
     CDS             26012..26425
                     /locus_tag="VAH76_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385075.1"
                     /translation="MLRTTIIGLLALSSLQIQAANITQVGRYATVNNQPLAAQINPLK
                     TVQQIHFPSSVQTIGEAVEYWLRYSGYHLAPQNKQNESLKQIFQQPLPQVTRNLGPLA
                     IADGLTVLVGKTLFSLKQDDLLREINFSLNARRAQ"
     gene            26422..27009
                     /locus_tag="VAH76_000423"
     CDS             26422..27009
                     /locus_tag="VAH76_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385076.1"
                     /translation="MKKFLDAKWLGLVVLNVLVIVFVCVKHQSSSINNNNLNGLEQKL
                     ITIQSQLNKPNQQPDLSPITDNIKQLGDFIQQMQNKDDHQLGDLFSTQQAAIKNQLDG
                     IRELLKHLDSQKQPIKMLPASELPFRVLSIDSVQEVSVASVAYDYKTQALEKGDSLAR
                     WKILDINFAKQTIEFENADKAHVLIHLTQQEVNNE"
     gene            27002..27823
                     /locus_tag="VAH76_000424"
     CDS             27002..27823
                     /locus_tag="VAH76_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HEO1385077.1"
                     /translation="MNKLSAIALSLLLGQQAIAGLYIPGIATSPIQQANNALAKAGLA
                     EFHDEVIEEKDFQLNDDQKQEAKVWGLNEKEEKRYLQLMQSRSGVYYKDLRMTPIDIL
                     GLNARDDAEREHFANLAARQEAQKVAQNIAWNNAFYKAYNELFKDVPVVGDFDPSPYS
                     PYAHQPIQLKEGETLYLFIKPDDAVTTIVLQLIDAINRTPNTKLNLLFLDMNNNAIQL
                     WANRHQIPIQLVTNQQITLTPGNQQFEGLALAKKQTPLLLIANGKTSQVVDLGRF"
     gene            27823..28254
                     /locus_tag="VAH76_000425"
     CDS             27823..28254
                     /locus_tag="VAH76_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEO1385078.1"
                     /translation="MIRILLLLSVALNVHSMNVIPLTLSQSDTSLKLNLSSPIGVPAK
                     SKATAGKVSRKTLDTTLLNIPLFVIGADKASIQWLEQHQEELKFMQATGFITNVNDFE
                     TIVALQEKFKLPLLPVNVDPLLAYLHEQHYPLIIAEGAVWQ"
     gene            28245..28877
                     /locus_tag="VAH76_000426"
     CDS             28245..28877
                     /locus_tag="VAH76_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HEO1385079.1"
                     /translation="MAVKQYSRQLTMQLTIVLFLGWLAIIIWALSQWMLHGYPIAFEK
                     VNILVNTQREAISPVYQGSLLAVLPLNAKPDWALAIPYVNKLVINDLAKDLIEKSQQF
                     LQILLLSSQCLLIKLIILFAALPLFALTMIAGLVDGLNQRAIRTACLGRESSYVFHRL
                     NHYLKKGLVILLMLWLALPVSITPAYVFVPISLLMGLMVAMTASRFKKYL"
     gene            28887..29213
                     /locus_tag="VAH76_000427"
     CDS             28887..29213
                     /locus_tag="VAH76_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385080.1"
                     /translation="MKRLLCLTLIMISLQCSANNPDLNETLVRIINQINAIMPLLDEA
                     QTQVTPNARIQLQIEGFEDLQGQHHSGLREDLLNIRNGLIDYINQPIIAPRKIKPLEM
                     DFVRKP"
     gene            29213..29479
                     /locus_tag="VAH76_000428"
     CDS             29213..29479
                     /locus_tag="VAH76_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385081.1"
                     /translation="MSKDEIARMFASTFKQASNNISAQVFSSSIRFIAISLVILAVMW
                     CVNHMMGTEEKQQQGYLLNFGSRMVRLVIGLMIFILVLFVKETT"
     gene            29476..29841
                     /locus_tag="VAH76_000429"
     CDS             29476..29841
                     /locus_tag="VAH76_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385082.1"
                     /translation="MKTTLRTLYLGAVNLFYAPVLLADNWFPKISDADNIGDGSKSMM
                     SVTGNYVKQGLGLLLFITAVVTFIKFITTVQHGIEESKKNEGSMVAFGTFAVMGITYL
                     AISIASGYVGYTMITKFKI"
     gene            29853..30215
                     /locus_tag="VAH76_000430"
     CDS             29853..30215
                     /locus_tag="VAH76_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HEO1385083.1"
                     /translation="MSQPSSRHLSHDFPAWKGLSLRELFWIVITTTPVTTLLFILLGT
                     MAGYPLAAGCIGFVIGFIFSITVWPKGVARIKAGKPYGYVMKKTIQWMVRLRLKHSPW
                     IHYQGQWQKNKSVGEPHV"
     gene            30208..30864
                     /locus_tag="VAH76_000431"
     CDS             30208..30864
                     /locus_tag="VAH76_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HEO1385084.1"
                     /translation="MFEYWKKIDSLQHHNRLLLAFIGLLSAIVLALIINLMTMPKRYE
                     FWLSPNMAANGGLMKASDIPNEYVQGFVSSLIPTLYTWSNKGKEEFNQNIKAFHYYFT
                     PRHEQLLRETLGAYKNAQLFDRSQVASLYRFMEPQDIRKIGPDTWEVKLILRITQRLK
                     DNSGMVIADKVVAYHLRVVKLKLSRLHNPFQLALDGYTRPEERLQDLLVDTEESHETH
                     "
     gene            30851..31627
                     /locus_tag="VAH76_000432"
     CDS             30851..31627
                     /locus_tag="VAH76_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HEO1385085.1"
                     /translation="MKLIKHILLIIGFMLTQVSYALQSEHLVWEKVPLTIELPINKER
                     LVQFPQAIKIIDQQLSTNLDILKVKGSLYLKAKDTFNDARLIVQLLPEGEVLILNLKA
                     NEKAANTTPIEILIDNPKTTHQSGASQYEYNAIQLTRFAIQALYSPERVRNIPEGIYR
                     SPMQTNKTIPLFYGASIEAHPLASWRGGSLYVTAIDLKNLLNQPVKLQFSKLMGHWQT
                     ASFYPKSELPPRNQHESTTVFVVSDQPFAAALTQHARYSR"
     gene            31624..32883
                     /locus_tag="VAH76_000433"
     CDS             31624..32883
                     /locus_tag="VAH76_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HEO1385086.1"
                     /translation="MNKNKGIKILAGSVVFAVVMILINSRHSTPIHDDTPNPNNFHEA
                     ITSQFNDNIRDVSARLLETEKKLEQMHKENKSLQKQLKQPIQEASHTLQDELQTLKEK
                     LSALTDHQTQSYPMEGTQAPVSGQLKDLDGLLDKAPIEGKIFADQDSSTKEKAPTKTP
                     FYTIPAGSDFSKVTLLSSLMGEVPVEGKLMQPLFPFTAMVSRGDLMTANGMQLPEEII
                     GMKISGYSIGVGSFLDNISCVRAYVTSALFVFEDGHFVSAGQEQMKSSAELVNNDSIG
                     YLTTQFGNPCIKGQYITNAPRVLAAFMAAGGVQGAGNALSKWQMSYQAEGGSAIATPT
                     GDLAHFAAGGAINEGSQKITDWLEKRIQGSFDMVFVPASIRSHSGFIPNQFSLHFSQT
                     IAIDKEHQGRVLDYGYNQQKNFDNALR"
     gene            32840..33187
                     /locus_tag="VAH76_000434"
     CDS             32840..33187
                     /locus_tag="VAH76_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HEO1385087.1"
                     /translation="MVTINKRTLTMLCASLALSACSTANISQSAIPEQGLTVSQLYQQ
                     SMQQPVQKAVVQQVRFENSGEEFKQLPNPEVPIHIFAHVAQLDDEQIIKPAYTTRFFL
                     YKQNQYALASELY"
     gene            33197..35947
                     /locus_tag="VAH76_000435"
     CDS             33197..35947
                     /locus_tag="VAH76_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HEO1385088.1"
                     /translation="MKRLSNWLLGEMNPQAIKELTIKKQYANPLPSFADRLAIVDFNE
                     QEQVFLFADGKSLGSGFELGDIQAEAASPEYLHAVFNKIRDTFATVVPLHAIDPWVMQ
                     MFVQDEYCLDPVIDHIKAQIPQQFIESSLTQDYLQRLQDLYNKMTRPEGLFLDPKTGA
                     PYRGRRRRIRVLFYRQLHQTTLTREQILLEHQEVISQIETKLRSPGLEIKRLKGQDYY
                     QWWIRWFNPKSADEILEQYPYPNHIPAGFNLAQNIFFSPPESDEQGFIFEGRKQRILY
                     VDGLKEAPIIGLVSRERQQANPKHRYALLDTLPEGSIYTIQVVFSHDENLDSHLTRLE
                     KGIVGTSLKPQEVRDDIKTARNELSIGNRLFWVNQAVFYQAEDEKQAQAIEKNLHTIF
                     NDAKMPLIHSSYDIHPLNSWLNALPFNFDPRYARKYLCFDRLMYATELAALLPVYGRN
                     QGARHLPCFPFFNRLGEPVFFDLLHHDFISQNSHCAIFANSGGGKSVLTGWMIQSLLA
                     MKNARVVLFEMGNSFDRMLVHAKAHGKKTKQLLLSNKKEEAVPLNPFCEAYKAIPEIT
                     DNLSAEQAALIAQKIMDLKDPAHSEDLACGVGSRAYLAELALALRTMITEANSMEEEQ
                     FTLADETLLIEVLIDAILTSFNEGIPQMLTEHIVSAFQRRLDKETVTRKKDRILDMHD
                     RLNSYVINSAKSRFFNVPTEPLGDFDIFHIDISAIKDDTGKLALVMVSLLPRILAMAE
                     ATQNDNRPTFLFIDESHLQFQIPSVVTVCLLVAKVARKLGLWLVAITQNVTDMNSEKA
                     AKILSLIETWILLGLDEKEITDVKRFKSLTPQQETLIRDIDSQKGLYAEAVLLGSRYQ
                     GLFRVIPPRYLLALLMTEKSEKAQRHLLEKEHDVLEAAEIMAKKLEQPKTTTNNRAYF
                     YD"
     gene            35940..36914
                     /locus_tag="VAH76_000436"
     CDS             35940..36914
                     /locus_tag="VAH76_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HEO1385089.1"
                     /translation="MTNTAFALESTSPPHPVNTFTIATRVLQKLFHNSHYKVIGSCTW
                     TVGRLPPRLEVTPAIEQFLPDLVITVSNKPEENPWIEARTLYENKAARTMYQQAYKAA
                     TGSALGFGNDSGQTTDLHINDERTRIVDVIGSPAAFYRIPYLSHKPETGFGVPYYLTE
                     ADAVMDRTEAAELLYMGTHPHLLINHEIGTFTQHWGSEIPRLMRVTQPYNYRASVVAA
                     MHAVDIVTNKNPLHVTKSTANSCGKNCVVSNAIYDPQNTKVIWQEIYPLNRNIHPGDA
                     QDFGIEDEKAGNGNYVFILWRKYRGCIQHKGKLVNFLTFPKVGQPQKR"
     gene            36924..38291
                     /locus_tag="VAH76_000437"
     CDS             36924..38291
                     /locus_tag="VAH76_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEO1385090.1"
                     /translation="MKKYLLLALLVPTLTHAGSFMPSQSDYYYELGGTSNLFIPPVNK
                     DQTLVIGADIDGRMGFSCSGFNPVVSITNTFQDLKKSALNIPGGVIDNLKGSVAGFPL
                     YKLQQSMPALYNVLQNAASSAQNEFTLKVKDCQEVKQALEQNQSPMEGILSVSDSQGW
                     LDAAKRAKKENVDVTETSKSIAQKRDEYGLPWIGSDKGNAGGKFQRPIKVINDVVIAG
                     YNILLERKPLDSLEKPSTKIPMVQSWPTPTDAANWAVKVLGDIQVSSSENKQSHDAKA
                     GIGLSALLQSCANANTCSQNIAKALWNLVNGTWTLTEENLRKVSASNLLITDEVITTI
                     QHLPREEQMLTVSKLAEEIAVQNMLDKALMMRRILQAGLQVQEVQNLKPAMNMVLFAL
                     KKLDDDIHSLSFENDVRKKMMTETVGTIMDIRHQAQSQNIPGQDHEQPAVKNGAIYVK
                     ENKGA"
     gene            38294..39847
                     /locus_tag="VAH76_000438"
     CDS             38294..39847
                     /locus_tag="VAH76_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HEO1385091.1"
                     /translation="MIVFNPLSLYTTYLGWQQYEVLFNALWQTGLLYLGFLAIGYRFL
                     KNVLNPAGAFYAVEHALNNFLYELAVTFLICSLFVYPCVPLETKALQFKPLCGLKNPT
                     TAVIGDSGTTYDEAFADLLTNQVKIPIGFAIIQNFMSSFTYSLMKVTGCTDSLQSIQG
                     DLVSTYLPQNIRKQALDFHRQCFIEARTKFNSEKHEASELDPMLKRYGGEDDLNWMGS
                     KILQKMYYSKLHARQPVPGFTFHQAPNRNLEKAANRGDIPPEQLPEDGYPSCQQWWHK
                     IKADLVDVSNQASVFNKHLNYYAMLDRVRKYKMKHPKAWKADISAEDFIAKMLLQESK
                     DMQASSVQNLMDNNNGKVASAITHSLVNVGQWTKSWTSTPLKREAIMQTLPVMQAFFM
                     FFLIILTPMVLSLSCYSTRALGSLCALFVMAIFLQYLWHLVGFLERSVLDPLGENDAI
                     SAMKNMAVMFYFVAPVLLLRLSSHFGGDAGAGLMDLVNGSDRQSESMAQSGMHVAKTG
                     VKIIAKGIR"
     gene            complement(39853..40026)
                     /locus_tag="VAH76_000439"
     CDS             complement(39853..40026)
                     /locus_tag="VAH76_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385092.1"
                     /translation="MRRVFRLLAVVLDAIVGVAEDKPDKRYMSVYEAQDKLDNGLITI
                     REYNEAFESKDVL"
     gene            40041..40370
                     /locus_tag="VAH76_000440"
     CDS             40041..40370
                     /locus_tag="VAH76_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385093.1"
                     /translation="MIKHYIRSSIIVLIQTVLPMIALLVFAPWFINSNLLARWQSTFT
                     TIQPLFLALHGVLYLALVLLWPKLISRLQNQHQLTAERLRTALNARWYLLAIIVFIDA
                     LMIGSRL"
     gene            40367..42301
                     /gene="traD"
                     /locus_tag="VAH76_000441"
     CDS             40367..42301
                     /gene="traD"
                     /locus_tag="VAH76_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HEO1385094.1"
                     /translation="MSNYPINNLLREPTEAWSAITCLSLSLLAYTQPHLFLLTQTMGL
                     YAALALLIPGIYRAWQAIKVKRYHRRLLAMPTYALSTTEIPLSKNWLFLGKGFRWRPS
                     HTQKLHQIKQVKNEKFMQRNYCYQLARRFCQNHEHTKLTKWLNTNSKLNPFRPDPPVG
                     GSPFLHGVGEEDKPVYIPQDVRVGHTFVVGTTRVGKTRLASILINQDIRNGDAVIVVD
                     PKGDQDLVRDMMAACKVSGRTEDFKIVHLGFPEQSAQYNPLKNFDQISEVATRITDAI
                     SAEGEGKQFAAFAWKYVNIVAICLEEMNQPITYQTIAFYISRLEQLLMSYADNILPNH
                     YHNYHQEIDEIIKEHDSKIGKNNKTKSPMERHQAVVQYVKDHINKTIKSNNAEALHDQ
                     ILIDLYDAAIMDKHYYDKITASVGPVLSEINKSNASKILSSKAQPDEIELMDVIKNKK
                     VLYIGLDSLTNPNIAQAVGKAFLSDLVSTAGKIYKEPNARYTLNLHCDELSEIIQDSF
                     VKILNKAGGAGFQVTAYAQTIQDMEVALGSRAKAEVSEGNFNTLIMLRVKNEETANLL
                     VKVLPQVGVVGHTQVSMVNDTPHGDDRVYFNTTNEDRVQTSSVPMIGVDDIVSLPKGQ
                     AYLLMSGCEIYKVRIPLPNH"
BASE COUNT        13278 a         9279 c         8072 g        12012 t
ORIGIN      
        1 atgatattga atgatatcgt tttatatgat aattttttta tgatgcttga ttacaaggat
       61 aattttatga gtaagggacc ctatcaaacg tttgctaacc gcttgattag caccctcaaa
      121 gagaaaggat attcagcctc tcgctcgcca aatggaatat gtattaaaac cctggctgag
      181 tttactggcg cctctgaaca aatctgccgc cgttatatac gaggtgatgc tttacctgac
      241 tatgaaaaaa tcaaaaaact ggcttatcat cttcaggtaa gcccaggctg gttgctgttt
      301 ggtgatgatg aaaatacaac ttataagaaa aatgaaatag atgaaacatt acttcattac
      361 attttgaaac aaagtcatca tttatacccc gtctctcatg gagctaatga cgattacgca
      421 gattttgtgt taggattgat caaagaagta aaaacaatcg acacttcgga aaataattta
      481 ctaaaaatca tagacttggc cattggttca atttcttctt acgaagaaaa tagaaaaaag
      541 cacagtcatg gaatttaaaa tatttgtagt tatggatgta acagctgtgg aagtaaagct
      601 tcaccccaaa gccaaggaat ttctgtttga acactttgtc acaatacgca gggtgttttc
      661 agatgtacta gggcaagtag aaactgacta tgcttctatt gcccttatta accaggcagg
      721 acagatcttt ttcatatcct cgaacccagc catagagcaa aatttaattg agcaaagtct
      781 ctggctattt gacggttgct atcaaccaga attcctcagc caggatcaac cgaaattatg
      841 gagcgaacta ccacatatag gctgcacaga atcaattgta aaatacaaac aaataaaacc
      901 tagacttata acaggtatct ccatccccac tgaatatgat tcttataaag ctatcttttc
      961 atttggatta aaacggataa atccatatat ccagaacaaa agctcgattc attgcgaaaa
     1021 actgttagcc atgggcaaat ttgcgttaag gcgaattcaa gagtatttaa ttttccctga
     1081 caaacagccc tgcatgacga ccaagcccaa actgacatta atcatcaaca atcaggtgcc
     1141 ccatgaacat actccttgat aaaaacgacc ctattttaag acaaaccgct gatccaattt
     1201 ccgaatcaga atttggtagc agttggttaa aagagctgat taagactatg ttcggcataa
     1261 tggctgacaa gggtgcagta ggtgtagctg ccccccaaat tggcatcagc aaacgagtta
     1321 ttgtatttgg tactgattac accaaaagga gaaaaccaga atacccaatt cctgatacag
     1381 ccttaattaa tccttcatta aaaatcctat ctcaagaaat tcaaactggc tacgagggtt
     1441 gccttaattg tggtgaatta atgggtgaag ttccaagagc catggaaatt gaatattcag
     1501 ggtttgacat agatggaaat agaattacca aaaaagcttc cggtttagaa gcccgcattc
     1561 ttcaacatga aattgatcac ctggatggat ttttattttt agaccgagtg gaagatcaag
     1621 attcactaac cactctatcg gcaatgcaaa gtaaaggata atcgcatgaa gcaccaaacc
     1681 cgaatcattc tggcaggtac actgggtaat ttaattgaat cctttgacat ggctatttgt
     1741 ggcctactct cagtttacat tgccaaatac ctgattggtg atgtctccaa gggtttgttt
     1801 ctcgtctttc ttactttctt tgctggatac ctagccagac ctattggggc tatgataatg
     1861 ggcttactat ccgatatcta tggcagaaaa attatccttg caggttctat cctaacaatg
     1921 ggcatatcaa caacccttat cgggtttatc ccccctcaca gtactatagg tacattttct
     1981 gctattacct tattagtgtt aagaattatt cagagcttct cctgcggtgc agaatattta
     2041 aactcctctg cttacctagt tgagaatgcc gaagcctcaa aaaagggcta ctcaggaagc
     2101 tgggcatcct ttggcgctat gtcagggatg ctgatagcgt ctttagttgt attgattgtt
     2161 acttatttta ccagtcacta tcctgaacag gactggatga tctggcgtgt tccttttgtg
     2221 cttgcgcttt taggatcttc gattggttta tacatccgtt tatgtatccc cgagagtatg
     2281 gaatacatcg tatactatgc agacagacca aagcctaaat ttaagagttt actttctgaa
     2341 tcggtaacct atatcaaaaa caataaaata caatcccttt atgtgtttat tttgagttgt
     2401 ttgggtgtaa caactacatt tcaaatttac atttatggtc ctatgcaagc tcgtttatat
     2461 ggtaattttc aaaaccatga aattatcatt tccaacatca tttctttaat ggtacttcta
     2521 ggagtatttc ctctgattgg gaatctatct gataaaatta gtagagaaaa aatcgtagtt
     2581 gcagcaagta taggatttct agttctatct caaccttttt tcaacttctt atcgcacaaa
     2641 atattttatc agttaatttt gagtcaagct ttaattgctg ttccagcagg agcatattat
     2701 gcaacagtac cagtaatgtt agctgaaatg ttcccgatta aattacgttg tagtgtttta
     2761 tcaatactat attccattgc agcaagttta tccgctggcc tcgctccatt actttctatg
     2821 atacttgtaa aaaaaaccgg cgttgcatcg tcaccatcaa ttttagtttt tacgctaata
     2881 gcaacaatgt ttataataat aatttacaga accataaaac ctgaaaagag atgctcttta
     2941 acgacactta aaggctaaaa taataacatc gcatggcaat gtgcttcgga taaaagtacc
     3001 gctcagatta gttaatgata gtgttgcaaa aatattccca ataaatgagg cagattctaa
     3061 cttttaggtg tatcaatttt aacctaaatg aattttcaga aatttaaacg gcttatatct
     3121 agaacctaga tagaaaataa caactccgac tgtaaaggtg ttaatctctg aaatgacgag
     3181 ctgtgcttat cccattacaa ttaaataagc attaaaaaat cgtgaacgaa aatattattt
     3241 attatgttta aataatttct cattgaaata atcaaatcgt tattttcagc atagggggat
     3301 aaatactaac tcgtatgata atgaaagagg aaccgcctca cagctaatcc tgtctaacca
     3361 gagcctgcag gcgctcttga gcttgcaagt gaccttgcgc agcagccttt cggagccact
     3421 ttattgcttc catgctattt tgaggaagac tcactccatc atgataaagt gcagcaagca
     3481 catattgagc ttggggcaat ccttgactag cagctaaatg aaaccatttc agagcttttg
     3541 tgtgatttcg aatcacccct ttgccgttaa gaaagcctat acctatatta tattgagcca
     3601 cagcatctcc ttgtttcgcg gccagacgaa accatttcat cgcttgaaaa tcatcctgtt
     3661 gaacattgct ccccgttgcg tacattaatc caagtgttcg ttgagccatg agatcgccat
     3721 gtttgcctgc tttacgaaac cacttaactg cttccgtatc attttgccga acccccttgc
     3781 ctgtgacata cataactgca agattatatt gggctatggc atcttctttc tcagctgcct
     3841 tgtgaagcca tttaaaagct tcgacataat tttgttgccc attttcagca gtggaatata
     3901 ggatgccaag actacgttca gcctctggaa atccttgtgc ggctgcttta tgaatccact
     3961 tgattgcttc cctatcgttt tgctgagtac ccataccttt ttgatacatg acaccaagat
     4021 ttaattgtga cacggcatca ttttgaagag cggctttacg aaaccattgc atagccttgc
     4081 cattatcctg agggacccct ttgcctgcag catacatcaa gccaaggttg cgctgggcca
     4141 tggcttcgcc ttgtttcgct gccttgcaaa accattttac agcttcagag tcattttgtg
     4201 ctactctttt gccaatcaca taatctacac cacgattatt ccaggctgat gggcttttat
     4261 accctattat catattttct atgggttctt taatgacatt cactatccca gaataacttc
     4321 ccacaccaat aataattgcg gctaaaacta agatgacaag acgtaaacgc atactaaact
     4381 aatccttgtt agagtcgtta ataaaaacta aaatgagtct ccaacctcat aataagttat
     4441 tctgttgact gagctctatt atgacaaaaa ggaattatgc tactaaagat taggcatgta
     4501 aaaattttat gacaaaatag taaaagtaca tggacaaaga taaggatagg aatcacatga
     4561 acgatatcaa gtcatttaaa ataaaaaaat aattcttctt aaaattattt tttcggcaat
     4621 tttttcctgc acaacactca atgcctatgc tggcatgtaa tcctatcacc tccaatacca
     4681 taagaattcc aaaataaaca atgtttcctt atccaatact gaagtattaa tagagttcca
     4741 ccgtggtggc aattcactgg gaagactcct gacaaaaacg aatgagactc aagacatcac
     4801 catcgacaac acttatggga gcaatttggt tattcacgtg ctattcatcg agaaagaggg
     4861 aaaaagaata cgatgttggg gagtatctgc tgctaaaaca accattgaga ttcaatgtga
     4921 taaagtggtt tcattgaaaa aaacaaaggg aaacactgga gagtccactg attcaatcca
     4981 ttaaaccact aaaagctttc tggttagcaa gtattgacaa aaagaacagc gtcgcatgac
     5041 actatataac ccacttgatt ataaggaaat aaacaaaaaa tgactaacaa aattcgtggt
     5101 aaagtcaaat ggtttaataa agacaagggt tttggtttta ttgaaagtag tggcaaagac
     5161 tactttgtcc actttagttc aattcaaagt gacggcttta aaacacttcc cgatggggca
     5221 gccgttcttt ttaaaatggg aaaaggacag aaaggacctc aggctgagga agtagaagtt
     5281 atcaaataaa tgaccaccat taggtctagg taagaccgtt ccgatgacaa taacacttat
     5341 ttttactgca tgtaaccatc aaggaggaag gaagtgaacc aacgagttgc tttagtcaca
     5401 ggtggtactg gtggtatagg tactgccata tgccaagaaa tgcaaaagtt agattatcaa
     5461 gtcatcgcct gttatttcaa acagggcaat catgacttag cgaaagaatg gcagaaaaaa
     5521 caagccatgc aaggatttga tatcgacatt gtctatgccg atatttcttc cttcaatgat
     5581 tgtgagaaac tgaccgactt ggtcatcgaa aaatacggca aaatcgatat tctagtgaac
     5641 aatgccggtg tgacgcacga tgccactttg aaaaaaatga ctcaagaaca atggcaggat
     5701 gtcatcaatg ccaatttgaa tagtgtcttt aatatgacga aaaccgtgtt atccaatatg
     5761 ttggataata gttatggacg tattatcacc atttcttctg tgaatggacg caaaggtcaa
     5821 ttcggccagt gtaattacgc agccactaaa tccgcattat acggttttac caaaagtctg
     5881 gcacaagaag tcgcaaaaaa aggaattaca gtgaataccg tctctcctgg ctatatcaat
     5941 actgagatgc tcgcctctct aagggacgat attttagaag cgattgttgc ccaaattcct
     6001 gtgggacgat taggcaaacc ccaagaaatt gctcgtgttg ttgccttcct tgcagaggag
     6061 caaagtggtt ttattactgg tgccaatttt gatattaatg gtgggcaata catgtaactc
     6121 actcaaaagt ccattgagca ctgttatttt aaacattgat aagatggccg ccatccacct
     6181 agaggatttg ctaagcaaat ggatgcggct ttattggatc ggatgcttac taatatacag
     6241 cgctcctatt gttcaaagaa gagcgtttta tttctgcagc acataagttc ctggtgcctc
     6301 tggcagtgac ggattcatga taggtgatgc aatgcgtttt ttagcgtttt gctggactag
     6361 ccactcatgc cattctcgcc accaggaacc ttcccgcctc tctgccatcg caagccagct
     6421 ctcaggattt aagtaagcct tgccttgttt ctgctcatga acacgataag agcatcctgg
     6481 gtgatccggc tcactgatga tgcccgcatt atgcccacca ccagtgagaa caaaggtcac
     6541 atccccttcg gtcataagat ggattttata aacggattgc caaggggcta catggtcttt
     6601 ttcagtgctc acagcaaaaa caggcaattc gatgttctca gcggccaccg gcttcccttc
     6661 cacggtataa cgcccttcgg caaaatcatt ccttaagaag agtttttcaa ggtattcact
     6721 gtgcattttg taaggcatcc gtgtggcatc cgcattccat gcggttaaat caatcatccc
     6781 tcgccacatt ccatgcatgt aatcttgaac cattttggac caaattaagt cataagcacg
     6841 caacatctga aaagaaccag caatttgctt ggtatccaga tacccttgtt cccgcatcat
     6901 acttttaaga aaatccactt gactttcagt tacaaagagc attaatcccc cagcctcagt
     6961 aaaatcccct tgggctgcca agagtgtcaa gctgtttaaa cgttcgtcct tgtctctgcc
     7021 catcgcagct gccgtaatca tagccaaagt accacctaaa caatacccca tcagattgat
     7081 tttggtttct gggaaaaggg ttgataccac atcaaatgca gccatagctc cttgccgata
     7141 ataatcatcc atccccaaat cctggtcctc tttgtcagga ttgcgccagg aaatgataaa
     7201 tacgatgtgc cctgacttac gagccacttc accaaggaat tatgtgggga taaatcaaga
     7261 atataatatt tcataatcca ggcaggcatg atgagtatcg gctcttttta gacggccttg
     7321 gtttgggctt catattgaat caattccatc aaatgatttt ggaacaccac ccgccccggt
     7381 gtaatggcca cgtcttttcc tgggataaaa tgttcagcgc ctttaggcgg tgcgcctatt
     7441 aacttttcta accaatcttc aaatgcgatt tggctgcctt ggatgagatt aagtccccca
     7501 gtgcgcatgg cttcatgaaa taaatcagga ttagaccaaa caaaattgga aggggatagg
     7561 gcatcaaggc attggcgcgc ccagaatgaa acagtacgtt ccacttggtt gggtaatcct
     7621 ggcacgtccg ttgtggcgcg tcgccaccaa tcttccatct gcaaaaaccc ttcggcgaat
     7681 aaacgccaag gcatatgctg ccaactgtct tttttaaaac gcacgtcttt accatccgca
     7741 gcacgctcaa cacaaacgat attattaatg caatccttgg cataaaaaac cggataaaaa
     7801 gcaagttccc acaaaacccc aggggattgg gccaattgcc aaagccaggt ggaataagaa
     7861 gaaccgatag cagcaggact tattcctgcc gtccatttag acaagttggc ttgcactaat
     7921 cgagtgaaaa atggaaagaa gttatcggaa gtacctctat cagccggtgc acaacacgag
     7981 aaatctaccc cttcttgcaa ttagtgagta ttcttttctt aggacagcat tgtttttcta
     8041 gtcccttcat ggccgacatc cctgatatta tgctctataa tcaacacgat ataatacagc
     8101 atagcacaag atttaaccca tcgattttgg ccaataaagc ggggcttatt aacttatcca
     8161 tttggaccaa attacttttg gcagctcagt tggtgttatt ttttgatggg aacgaaccga
     8221 catgaagcta caaccctttg gagttccttt cctcgataaa ttgcgtcatc tgtactactg
     8281 ttaacggttt actactgtaa tagccttgca cttcgaaaca gtgacttgac cgtaaaaaat
     8341 tgagttgttc ttctgtttcg acaccaccca caaccacatt caatcccaat tcttgcgaca
     8401 aagcaatcgt cgatttgata attaaattat cattttctct gagatgagca tcttcaatta
     8461 aggcttgttt cagtttgatg gattggatgg gtgaattttt taagtgcgat aaaaaagaat
     8521 accctgagcc aaaattatca aaagcaagtt ttatccccat attgcgaagg gcacttaatg
     8581 tctcttcaat ttttcccaaa aaattgactg ccgttgcttc cgtaatttca agctctaacc
     8641 aatccgatgg catttgatat tcctgtaaaa tgtgcttcac agaggaaata aaccctttgt
     8701 gctgaaattg atgaagcgaa atattaatcg ccaaggtaca attgaaagga gcgtatctct
     8761 ttttccaatc cacatactgc ctgcacgcgg ttcgtaaaac ccattcacca attggaataa
     8821 tcaagttaaa atgctctgca acggcgatga attcatcagg aaaaacaaca ccccgcttgg
     8881 gatgttgcca acgtaataag acctccatcc caaccatagt attcgttttt aaatcaaccc
     8941 ggggctgata aacaagaaaa aattcttgtc gctccaaagc aagatgcaac tcggttccta
     9001 ttcctaagcg ttttgaatat tgttttctaa gggatggtgt gaagatttgg caattatttc
     9061 gaccacattc tttggcgttg tataaagcaa tatcggcatt cttacgaaga gttattgcct
     9121 catcaccggc atcaggaaag taagcaattc ctatactgat tccaatcatg actgtatgac
     9181 ctttaatatg gaatggctta cccatctttt gaatcatatt attggccact aattttgcct
     9241 gttgtgggtt attaatttct gtcaaaataa cagcaaactc atctcctcct aatcgagcca
     9301 cacaatcttc ttcgcgaaga ctggagcaaa gacgctttgc tacttcacgc aatagaccat
     9361 caccgacatc atgaccaaag cgatcattca catttttaaa aaaatccaaa tcgatgctta
     9421 acagagccag atgacgaccg tatctttttg acctggcaat ctcacgtttt aaatccagtt
     9481 caaactgcaa tcgattgggt aatcggctta atacatcaaa atgggctaat ttcgataatt
     9541 ttttttgtac cctctctaaa tcttttagcg tttttatcag ttcagcattc tttttttcca
     9601 aatcctggtt cagtaaatga agttcccttg tcttattttc caataataat tcggcttcct
     9661 cgcgagcttt tctttcgcga taataagagc gctcaaataa tgctttgttc ccatcactca
     9721 aaggtaatct ccaaacgaca atgatcatct tttttcaaca tgcagtgagt ctgattaatg
     9781 gtaattctct ttttaaaatg ctgcgctgct ccctggatga gacccattgc aaaatgacag
     9841 agccttctat gcgagcgata caccataacc aattggtttg cagcgggctc ttcatagtga
     9901 atggtaggta atgtttcatc cgggtataac ttttctactt ccacgtgaat tgttccatca
     9961 atgcttttta aaaattcttt taatgtcatg tcctttttta aaaaaattgc gtacttactc
    10021 gataagatag gaaacatgta ttcaccaaag gactctaaaa aagcagaagc gtgctgatta
    10081 gtcctcttcg ctatggcctg aatcaattgc tgcagctctg tatccgagta agtgccgcct
    10141 gctgtataac ttccaccact aggaaggtct agtgaggata cgagttggtc ccaggtttct
    10201 attccaaatt gttctataat catgtcattt aaggaggtaa aaacaatgcc cttcatctat
    10261 ggtctccagc ataaaacaaa tctttaaatc cctttaataa ttaagagaag tgttatctct
    10321 caatactaaa attagtatac ctacacatga atttctcgcg agcctgctca atttaactat
    10381 cgtatcaata tagttcaaat aacgcttatt taccgtgtta taaattaaaa taagatttta
    10441 tttttaccat ttcctaaaca aaactcaaaa atgatagaca taccgtgtgg ctatatatga
    10501 agcatagcac atatgctttt tgaatgaaaa aacaaaccga cttaattgta ctaggcaaac
    10561 aaattcgcaa aatcagaaaa gaaaaaggtt tttctcaaga gggttttgcc aatttcatcg
    10621 aaatgaatcg gggttactac gggaccgtgg aacgtggcga agccaatata accattttaa
    10681 atcttctaaa aatattgaaa ggattggatg tcacgccaaa cgaattattt ccaccttcca
    10741 cttatatgag tttgaagaag aaaatcacta gaacttagct tgctgttttt ttatgtgcct
    10801 aaccaagtcg tgtccataaa atgtcttata caagccaata tcaattaaaa gaacatcata
    10861 cattttaact gctaatttac tcaaagaatc ttctactgaa tcggcgatac ctacgtattg
    10921 cccaagccgc attaattgac ttcgaataat aagccatgca gcaagattct tatgctacag
    10981 gaaaatgaaa aaattcatta cattcacttg atttgcttaa ctcttaattc aaattataac
    11041 ttctttagta gtattccata tcacgccagg tccaggtaaa gaatagtgca gctcaacagc
    11101 taacggctca ataaaatccc aaaggaaact gctgttgagt tcgataggta cggaaagaac
    11161 aaactaggtt aaatcataag attgttagtt tagcacctat tcagaatatg agatcgctct
    11221 ctcggcctgg agagtaagta tcccatcaca tttagtaccg ccttgtaatg ctccaagaaa
    11281 taaaaaagca atgatgtcca gcttatacta atctttatca tcatataatt tcctaaaatg
    11341 aataaaaaag accttaatgc aattggaagg aaacaccatg taaaaaaatg gcagctagga
    11401 taaggaagat taatatggtt ataacaaagc gtaaattttt cttttttttc caaagaaagg
    11461 caagaaggcc aaggcataat accatcaatg ccaatagcaa tactatagtc aaagtattag
    11521 ctcccataat aatctatcct tatctggcta tagaggtttt taatgctctt ggtgcctatg
    11581 ccctccatca ccaccaaaac ctccctcgta accgctttca ggatgcgttt ctgtcgctgt
    11641 ggttttagtg caggacatta ttaagctaca tcctaaaagc aatactccaa tcaaaaagaa
    11701 aattgtctta aatccagtat tattcatcta cctctccctt tcaacaaata caaaaacagt
    11761 ggtttatctc atcataacat attgaaaaag caatcaaagc gcttgtatta atgattcttc
    11821 gtttgatttg aattcaacaa tcgtagccca ttcataacca caatcaaact agcccccatg
    11881 tcagcaaaaa ccgccatcca aagggtagcc attcctccaa gtgctaaaat aaaaaacacc
    11941 gttttaataa ccaaggatag actaatgttt tgccataaaa ttcgcactgt cttccggctt
    12001 aagtcaatgt acagaggaag catggctaaa ttatcattca ttaataccac atccgctgtc
    12061 tctaaagcgg tatctgttcc cttgcccatc gcaaagctaa tagtggcttt cgctaaagca
    12121 ggtgcatcat taatgccatc tcccaccatg cccacagcct gatattgttc taagagttta
    12181 ttgatagcct gcattttttc tgttggtaat atattagctt ggatttcatc aatgccgact
    12241 tgtttggcaa tggcttgagc ggttagagca ttatcacctg ttaacatcgc cgtttttatc
    12301 ctttgtcgat gcaatgattc aatcgcctct cgactgttgg ctcgaagcgt atctgccact
    12361 gcaaaaatag ccaagaccgt agtgctattg cttaatataa cagtagtttt tccttcttct
    12421 tccagtcgtt tcaattcttg ctcaacctcc aaacaacaga ctttattgtc ttctgctaat
    12481 tgatgattgc ctacataata caattccccc tggatggcac ctttcacgcc tcgcccagga
    12541 agcgctgaaa actgctccac tgaaagcaat gatttatcag gatgctcttg gtgccaatat
    12601 gccactaggg catgggctac ggggtgctcg gagtggctgt caagacttgc agcaagcaac
    12661 aacaagtgtt cattggtttg acttttatca taagtaataa aatcggttac caccggtttg
    12721 ccctctgtta aggtaccagt tttatccagt gcaattaact gtaagcgata acccgtctct
    12781 aaatagcttc ctcctttgat gagaagtcca tgtttggcag aggcagccaa accactcacc
    12841 actgttactg gtgttgaaat gaccagggca caaggacagg caataactaa taaagtcaac
    12901 gccttataga tccaatcata aaaaggatat cccaacacga aaggaggaat taaagcgaca
    12961 agaatagcaa tcaataccat aataggcgta tagtattttg caaattcgtc aacaaatcgt
    13021 tgcgttgggg cccgctcggc ttgcgcctgt tcaatcgctt taccaatttt agcgagcagc
    13081 gtatcgcctg acgctttgct cacttttgct tcaaaagccc catgctcatt taaagagccg
    13141 gcaaaaacag aatcacctat ctgcttactc actggcatgg attccccggt aataggagct
    13201 tggttgactg tactttgacc tgaaaccacc tcaccatcca gtggaatacg ctcaccaggc
    13261 ttgactctaa aaacagcacc cagtggcacg tcctcgacca gtaaagtttg ccattgacca
    13321 ttagctgttt tcacttgagc cacatcagga acaatctgca tcaagcttcg aatagccagc
    13381 cgtgctttgt ctaaggaata acgctctatt cgttccgcca acgcaaacaa aacagtaacc
    13441 atagccgcct caggccaatc accgataaag atagcccctg ttatggcaat gagcattaga
    13501 ctgttaatgt tcatggcttt tgtgcgcaaa gcaagccaac cttttttaaa agtaggaggt
    13561 ccactgagaa taatggccag taaggcagga ataatggccc atagagattg ctctgttgcc
    13621 aaaagataag ctgctatttc ggagcaaaga gccaatagac ctgataatgc aataatctgc
    13681 caagaggcat caaatttaag tttttcctga accttgtcgc taggggtgct ttgtttggca
    13741 cgcacgctca tccctaaggc ttctatctgc tgttgtatag gttcaatggt gggtaatcga
    13801 tgatgaaccg ttacttcttc agcaataaaa ttaaaatcca tcttttccac ttcagggata
    13861 gcttgcaatt gccgcctgat taattgttct tcagcctgac aatcaagtcc tgcaacaaaa
    13921 aaagtggtta aggtgagcgg agctgtcatc atgatttccc acattgactg taaaaatatg
    13981 cccccggagc aagagaagtt cgacatcgca ggcaattcat tcctatcgaa acatggctca
    14041 ccgaataacg accccaatcc tcttgtttta gcaaacgcat gttaattctc cgtctcttca
    14101 gtaaagtgtt taagcatgtc ctctaaaata cagcgtacat gcgcatcata aatactgtaa
    14161 taaatgtgtt tgccttcgcg attagccatc aagagccgtg ctgagcgcaa cagactcaaa
    14221 tgatggctgg ttaaagggac agaaagctgc aattgctctg ccagatccga aaccgatttc
    14281 ggccctttta agcactcaat aagaagttta aggcgattaa cttcgcccat taaatgcaaa
    14341 atgtcactta acttgataac ctggtcttga gttaacataa ttaacaatta aacagttaga
    14401 taagtattta actgttaata tagctcatta gcaggaattg tcaaagaaca ataagctctg
    14461 aagccgctca ttactgcttg cataaaagaa cccagtgaca tctatactta agtaaatacc
    14521 atcaaatcgt gagaaacaac gatgctcgac tgggcaagcc ataattttct tattacagcg
    14581 gggataattt tttcaatagg actggttttg atagttcttg ccaaaggaag ctctactcac
    14641 cgcatgaaac tcattgcaaa cataggattt tatcttgctt ttgtggcctt agtggtcttt
    14701 gtgatagtcc tctttctgga tgtcttgccc aggaagcctt aattccaacg agaaaataaa
    14761 gggatcgtct ccatgttaat caagtgtctt cgccggcttt tttaatgggt aatttgttac
    14821 ggtaacgccg ctcatgaaag acgtaataca aacctggcaa cactaacaaa gtgagaaaag
    14881 tagaggaaat gattccacca atcactacgg tcgccaaagg gcgctgcacc tcagaaccag
    14941 tgcctgtcgc taaggccatc ggtacaaagc ctaaggaggc aaccaaggcc gtcattaaaa
    15001 caggacgaag cctcgctagc gagccttgta agaccgcatc tttcaaataa acttgtttct
    15061 gctcacgtaa tttattaata aaggtaatca tgacaagacc atttaacaca gccacccctg
    15121 ataaagcaat aaacccaaca ccggctgaaa tagacaaagg aatgcctcgg aaccacaaag
    15181 caaagactcc gccagttaat gccaaaggaa taccagtaaa caccaaaaga gcatccctta
    15241 ctctgccaaa actcataaac agcaacagga aaatgcctaa taaagtgata ggcactacga
    15301 tttgcaagcg ttgggaggcc gattgtaact gctcaaattg cccaccccaa gtcacccaat
    15361 agccagtggg aattttgacg ttttgttcaa tacgttgttt tgcttcattc acaaaagaac
    15421 ttaaatctcg atttcgtacg ttcgcactga ctacaacacg acgtttgcca ctttcacgac
    15481 tgatttgatt ggggctttcg ctgcgcacta aagtagccac ttcactcaga ggaataaaat
    15541 gccgctcgtt gtctttggaa ggaggtaaag ggataaagac ttggcgcaac acatttgggt
    15601 ttgagcgcaa ggcctccggc aagcgcacta ccaaatcaaa gcgtttatcg ccctcaaaga
    15661 gctcgccacc ttttttgcca cccgttgcaa tgacaatagc atcttgtacg gcaccaattt
    15721 gtagcccata gcgcgccaaa acgtcacgat taatcgcaac ggtcagaagc ggcaagccac
    15781 tgacttgctc tacttttaca tcagcagccc cgggcacttt ttttaattga gcactaatgg
    15841 cctctgccac ttgcagtaag gtatccatat tatcaccgaa tactttgacg gccacatcac
    15901 tgcgtactcc tgaaatcaat tcattaaaac gcatttgaat cggctgggta aactcataat
    15961 tattaccagg aatttgtttt actgcttgct ccatttcctg cactaattct tgtttggtct
    16021 ttttaggatt aggccaatcc ttgcgaggtt ttaatataat aaacgtatca gccacattag
    16081 gtggcatggg gtcggtagct acttctgctg tccctaattt tgcaaagact cgctttactt
    16141 caggaaattg gcttacccgc tcctccacta aatgctgcat ggtgatggct tgagtcaagc
    16201 tcgttccagg aattcgcatc gcatgcatgg caatatcccc ttcatcaagg ctcggaataa
    16261 attccccacc taaacgaaaa gcaacaagaa gactaacgac cactaaaact accgcggcac
    16321 taattacaag aaaacgtgcg tgaaagcagc gacgcaacac tctggcataa cctagtgaga
    16381 ggtaatgcac taaccagttt tctttttctt gcacacgacc tcgcaaaaaa atagcgacgg
    16441 ctgcgggaac aaacgtcaaa gcaaacagca tggaagcgag cagcgcaata atcactgttt
    16501 cagccatagg caaaaacatt tttccttcca caccggtgag cgttaaaatt ggtaaataca
    16561 ccaccgtaat aataaatact ccaaaaatac tgggcctaat gacttcggtg gtcgcatacg
    16621 caataacctt taaacgctct tccaaattta acacacgata taatgcatgc tgctgttcac
    16681 ctaaatgttt aatgcagttt tcaacgataa tgaccgcgcc atcgacaatc aaaccaaaat
    16741 caagggcccc caaactcatc aaattggcac tgattttatt agtgaccatc ccggtaatgg
    16801 tcaacagcat ggacagggga attaccatgg ctgtaataag tgcggcacga acgttgccta
    16861 agaacaaaaa cagaatgaca caaactaata gcgccccttc gagcagatta ttctttactg
    16921 tattaatcgt ggcattcact agcaaggttc gattatacac agtgactgcc tcaacacctt
    16981 caggcagtgt tttattaatt tctttcattt tggcagccac ccgctcagac acagtgcggc
    17041 tgttttcccc catcagcata aacaccgtac ccaaaaccac ttccttactg ttctcagtag
    17101 ccgcccctgt acgcaattcc ttacctaatg ccacctctgc tacatcccga atacgaaccg
    17161 gcgttccttc aaaactggca atgacaatat tttcaatgtc cgcgatgttt ttaacctgtc
    17221 ctggaacacg gattaaatgt tgttcgccat tggattcaat gtaacccgcg ccgacattgg
    17281 cattattgcg ctctaatgct tccacgatat cgtttaaact caaacgatat cgaacgagtt
    17341 tcattggatc aggtgtgatg tggaattgtt tttcatagcc gccaatggta ttgacttccg
    17401 caacaccttc gacattgcgc agttgtggct taattatcca atcctgaatg gtgcgaagct
    17461 ccgtaggatt gtagcgttga ctggcaggca cattcgtttt attcgtcacg gtatacataa
    17521 aaatctcacc gagacctgtt gaaatcggtc ccagcgttgt ttcggcctca ggaggtaatt
    17581 tatcctttac ttcctgcaaa cgctcattaa tgagctgtct tgcaaaataa atgttagtgc
    17641 cgtctttaaa gaccacggtg acttgcgata agccatagcg cgataaagaa cgcgtataat
    17701 caagactagg taggccgctc atcgccaact cgataggaaa ggtgatgcgc tgctccacct
    17761 caaaaggtga gtagccagaa gcctgggtat taatttgaac ttgcacgttg gtgatatcag
    17821 gcaccgcatc aataggcaag cgctgaaaat tgtatacacc caaagcggca atcactgccg
    17881 taaataataa gacaaaccaa cgatgtttta gggaaaaacg aataattttc tcaagcatca
    17941 tccatcctta gtgctcgtgg cttgcgcctt ccttgccaag ttctgccttc ataaagaagc
    18001 tgtttttgct gacataaggc tgaccaacat caagacctga caccacttcc acccattgtt
    18061 catccttttc gcctaaaatg acgggtgttg cttcaaaata atcaccttgc cgcacaaaaa
    18121 tcacgtcctt atcattcaaa cgctgtattg cagaaagaag aacggcaacc ggaactgttt
    18181 tttctttaat ggtaatcgcg ccattcacat acatgcctgg caaccagaga cgctcttcat
    18241 tagaaagtac agcacgtgct aaagtggttt ggctgtcttc aattcccaaa ggcgcaatgt
    18301 aagaaatgac actggtggac tttggttttc cctcatctcc tgtcacaaca acctccattc
    18361 cttgttttac ccgaggagtt tctttgcggt ataaggtaaa gtcagcccac acagtagtta
    18421 aattagccac ttcataaata ggtttcgtgt tttgcgcctg ctccccattg gtagcatact
    18481 tttgtacaat ggtccccgtt atgggtgcgg taatatcgta attttgcagg ctttcattac
    18541 tttcaatggt gaccaacact tcgcccttag tgacctcatc gcctaaattt ttagtcaacg
    18601 ctttaataat cccagaataa cgagggtaaa tcggagcaag tgtatcgcga ttggggacaa
    18661 tctttccgac cacttttaat tgtgttttaa tcgtttggct ttcagcaacc gaagtttgaa
    18721 tatcagcagc ttttaaaata gccggtgcta ttttgattcg tccttcatag gtggaataat
    18781 gccaggacaa tcgtttgccc tttagctcca attgaatcgt gacatcaaag gagtggggtt
    18841 ctccaatcac ttcattactt tgtaaaaaat tctcaacggg aatgaaggta atggtctctt
    18901 ttttaccatt aaaccgggtg agataaacaa ctaaacgggc cttggttggt ataatcagtt
    18961 cacctttctc ataaacaaag ccacgaaaat gaggaggcat accccgttca aaaatcaaaa
    19021 gctcaagggc tacatcacct tccttgaata agcgaccacc ttgagggcct ttttccatga
    19081 tttcttgatg gttgccttct tccatagcat gagagccctc ccctgcccag gaaaaaaccc
    19141 ccataacgaa ccaaagaata agaagcagaa tggatggctt aaatggcttt aggaatgtca
    19201 tttgctctcc tccttagaat tatccagtcc taaaagtcct gtcatctgaa ttaaggcttt
    19261 atgataatcg gcatgagcct gctggtaatg ccgctcttct tcatacaaag aattgagtgc
    19321 ggttgataat tcaatgtacg tgtaacgtcc catttgatag ccctcttgag ccagttttat
    19381 cgctttacgt gccaacggca ataaggaatc cgtaactaaa ctggcttcat agtggttttg
    19441 ctgggcttgt aaaaaggccg catagacact ttgacgcaca tcaagacgcg ttccttgaaa
    19501 ttcatgggcc gtctgggtgt attgcgcttc tgctgtcatg attttgcctt gatttcgatt
    19561 atatactggc acttctgcaa aagctgacat aacggccgca ttactgccgt catcagaaaa
    19621 atgacgacct cccaattgaa tgtttaaatc cggccaaacg gattttttaa cagcggtaat
    19681 cgtcttgcgc ttggcttgta attgcagttg catttgcagt aactgagggc tttgcggtaa
    19741 tttttttact aattcagtcc aattgactga aacacctggt aaccctttat ccaccaaagg
    19801 cctatccact cttaacccat cacctaacaa gcgcgcaagc ttggctcgct gcaacaaagc
    19861 gtcccgtgct gctttatttt cctggatttt ggcctcgccc aatcgtactt gcgccaatcg
    19921 caaatccagc tctgccccag cacctgcctt aacccggcgc tcaatagcca ccacaatatc
    19981 ctgattcagt cgagtcagtt ttttggtcac ttgatgccat tgaccggcgt atagcgcatc
    20041 cacataagcc acacccacag ccatatacag cgctgtttgc tgaactctga tgctggcaag
    20101 agacgccaga tagtctgcat aggttgcttt tttcaaatac tgaagtcgat ggcctaaagg
    20161 aatcggctga gtgactgaaa acgtggtttc cgcagattca taactggagt aggcgccaga
    20221 gccaccgaaa ttctccgccg ttaaagtcag ttgcggattg ggataaaggc cactttgaat
    20281 aaagtaaccc cgcatggccc ttgctttgtc catttcggct tgcaattcag gattatttcg
    20341 ataagccgtg tccaaagcct gtttaaaagt aaagggggta gccgacagta cttcgcaaac
    20401 aacgaagtac atcatcatga aaatgacgag acgtgaaaaa taacgcattc cttacctttt
    20461 aagaaaatcc atgtcaaacc ttattgtttc attgtacctt gatttcgtgt cttgatacac
    20521 cctattgcta aaatcaagac aatctgatgc taatgcactg aaatttttct caaattgaga
    20581 tacactgcgt tttatggtcc ctcgtcattt tatcccatgg tgatacgatt attctttatt
    20641 ttattaatac tcgttaccgc tgaactaagg actcaatcat taaccattgg gattgcagca
    20701 aacgactcac gcgcagataa aaatcatttt ttggatttga tatcgaactc atgacggaat
    20761 tgtgcaaaca aatcaacatc cagtgtcaat ttcaagccca tgctgtttaa cacgctgttt
    20821 actaacttaa atgccaggta aaattgacct cgccattgca gcaattacta ttaccaacga
    20881 acgacaacaa gcctttcttt ttagtcttcc ctatttagcc agtgaagcac attttgtcac
    20941 caccaaaaac aattcgctca agagccttaa aaagcttacc cataagaaaa caggtattat
    21001 caggggctct ctctataaac aattaattac ccagaaattc aaaaccaact cgattaaaga
    21061 atacccttcg acagataaat taataaccac cttaaatcaa tatgaagtgg atgttctgct
    21121 tatggatgat ttttctgccg attattggat agcaaaataa caaccggtta tttaaaggag
    21181 ttggggacac cattccgatt ggaataggtt atggtatcat ggctaaaaaa gattcgatgg
    21241 cgctaattaa caaaatgaat caagccctaa aacaaattga aagaaatgga atttacttgt
    21301 ccttgtataa caaatacttt catacgatac ccatgcacta attgtctata ctaattagtg
    21361 ttaattaggt cctttctaac gagttccacg cccatggaca tgaggatgat agccaccaaa
    21421 tcgcttctta accggcgtgt tactttttga gtcctcttta gaaacggaag cgctgcagga
    21481 aaacaataaa aaggataaac aaactaaaag aacttccatt gattttttta ctaaagtgaa
    21541 gtgtttcatg tattttcttc ctttttatta agtttgatta ttgaatagta agagacagtc
    21601 tgttgattgt ggcatttaaa acgcttttag ccaaatcctg aggcgtcaaa tggaaagatt
    21661 actgatacga tttattgagt ataatggttt ttttactggt atcgctaaga tatactcgac
    21721 taaaaaatgg tctcggaaga acttaaatca tgagatactt gcgtcatcaa agcacatcga
    21781 gcatcattgc gctcatttta atcaaggagg agcaactatg aaaaaacgga ttattccatc
    21841 ggtctgcagt ggatttattt tatcctttat gacacaaatt gctatcgctg atgacattcc
    21901 aactcggtca ttgcccatgt cgcaaatcct gcaacacttg cagagtaagg gttaccatac
    21961 tatacaagaa gttgagtttg atgacggact atatgaagta gaagcccttg atgaacaagg
    22021 aaatcagata atattacgaa tgaatccaca aacgggcgat ctcatcaaaa atcaaaacac
    22081 tcaaaaatat ccaatatccc tattagaagc cgcccagaaa attgaagggg ctggttatca
    22141 ttctatttac aaaatagaat ctcatggtag ccagtacgaa gtgaaagccc ttggcaaaga
    22201 agatacaaaa gtaaaattaa gagtagatgc caataccggt aaaattaata gagacgaatg
    22261 attcgattag gcgtattaat taaaatgctt aaagacagct gatttttctt tcaataagta
    22321 gttataactg acactacttg tttctaaacg gtgctatatt attaagaaag atttataaga
    22381 tgagatggat tgtttttcta ttgattccac aatcacttta tttcattgca agtagcatgg
    22441 atatatgcca aaacagagat tttttaaatc cgttattggg ggtaatgacc ttattcagca
    22501 caatgctttt gttggcctct tgtgccccat caacaacgac caatgattag caacaacgga
    22561 ttaattattt taagcagatt attaccacct ttcaaaaaca agaggaagaa gtagccgaaa
    22621 atatgatggc aagtgacaaa agactttagc tcgtaaatcg ggtacacatc ttagccatca
    22681 ctatgcaaaa tggcgaattt attttactca attgaaagcg acattaattt tatattttta
    22741 caattaatac ttttattcac aatcaaagta agtaatagtc ttatatgggt atgttaaaag
    22801 cacgctaaat aaaaggaatt ataggtatga aaattgttta tttaataatt agctttatga
    22861 ttcttctttt gaattcgggt tgtggaggat tacgtcaatt aaatgcagat gcgaaaaatg
    22921 ttaacttgta tttttcctat cctacaggaa attgccgttt cttagggatt attaaaaacc
    22981 caaatgtaca tgaagttatg gatatccgct cctctttaaa agatctagaa aaagatgata
    23041 ataatttttt aaaaaatgaa ggcgcgaagt taggagcaaa tgttatcgtt cttataaccc
    23101 atacaccaag ggaagctcat aagagatatg taaggggatc aaaaaactta acaactatta
    23161 atatccattc cgtaatagct aatgcatatt attgtcccta tgtgaaaacc gaccaattac
    23221 aacaaagaag tttagaaatt aagcaaactc cattatttga aaatgatgat ctgcagataa
    23281 atcattaaaa aatccaggat cctgggttaa attacaaaac taaggataat ttaacccagt
    23341 atcgtcagat aaattgtcac catcctaaaa gacatgagga aggattttct tgggaagaga
    23401 acgctcaata cctcaataaa caaggttatt gataggtgat aatgttcctc attgatggat
    23461 tagtatcatg aaggtgacta tttattccta tgaattcata aaataattcc actgtatatt
    23521 tggcttttta aaaaaagaac agcaactcct aacgtgattg tatcttgagt agtagactct
    23581 ccctgacttc gaagaatcca cttaagagca ccatactctt catcatgttc gtactctgta
    23641 aaaatattaa gccctggctt aagacgataa taaggtctaa ggatataacg catttggttt
    23701 aagccattgc caatttcatt tttgactacg gtatgagtgg ccaaaatgct gcgaagtcct
    23761 gtgaggaaga aaaagttatt ggtcagttgg ttagcccgag ctaactgaat gtcaaatttc
    23821 acactgccat cacggtaata ggttctaatg ttggtatcaa tataccaagg cattaaccct
    23881 tcaataccaa tatcaggttg ccaataaggg cctcccgggc gataaaaata attgacccca
    23941 tctttaatgg ctgaactcac aggggttagg cctagcataa tgatttatcc tgtttgccat
    24001 tcgtctgtat gaacgctacg gtttgattga gacaccacaa cgtgccccgc caatatcaag
    24061 aagacgtcgt tgattgcctt caactttgcc aaactcagat tgcattaaaa gcgatcttaa
    24121 tattcaaata taacatgatc catccactct ccatgtgggt cattagaaat accaagcaac
    24181 caagaagcag caacaaccaa ctctttggat agaactaaag cctcttcagg tcctgggctt
    24241 ctcgtaccct gctcccaatt cccaatcctg gcagcgccaa atcctgttct ctctgccaat
    24301 attttaatcg tcaacccatt tgcttttcta gcctgcgtaa tcctactacc tataattttt
    24361 ttcatgttca tttaggttca tttgatatca aacggaaata gcccattgac actaaaaagt
    24421 gcttatttac aatgggttat caacaacacg tttcgtgtta ttgattgata ctaaatgaaa
    24481 ttatttgata ttgcaaacac tgaatttgga acagtgtttt tagatggatg aataagaaaa
    24541 tgggattttt gtttgttact actaacgaat tatccgcact gatgggattg ccttatatcc
    24601 agcaatcgac ctacttgctg gggattcgac cttatatgga tagggaaacc tttctggttg
    24661 gggttaaacg aaaaatcagt tatcagcaac tcgctgaggc tttatatatt gagcctcatc
    24721 aaggctttca acaatccggt agtcctagtc gtcaacaatt gcgaagaatc atctatgcgc
    24781 tggagagagc tggtttaatc aagattgagt ccataggcat gaacctgatt gtaaaatgcc
    24841 ttttagctga tacggatatt tctgtccaaa ataaacccga cacaaacccg acacagcaac
    24901 ccgacacaaa cccgaacatc aaaaagaatt acaaatcatc aagttacgat gatttttcta
    24961 aaaaacccaa cacagttgaa aacataaaac ccgacacacc tcataactca gaaaatttgt
    25021 gtgtgtatgt gcgcgcccaa tttcaaaaat tttgggaact ttatccacag aagcaggatg
    25081 agactagagc attccaggag ttttataaac tcaaacccga tgaaactctg tttaggcaga
    25141 tgatggatgc actgcatgct caaattgaaa accgtttaga cctggagctc tgtggtgagt
    25201 gggttcctaa atggaaattt ccagccaatt ggctcgccca acaatgctgg aatgatgatc
    25261 ttttacccgt aagaaaacag gagcaaggcc atgcaaacac tcaaacaagt tttagaaaaa
    25321 aatcagccgc agatattctc gctgaatcct gcaaagaatc cagcttcagc atcaactttg
    25381 aagacgaacc ccaaacagga aacaacgtca tccacttcga ctcaacaaga agcgtatgac
    25441 aaacgggtgg atactttgtt cttaagattt aatgccatct atggtgcctt atggctttct
    25501 gcttataaca atgaaaaagc ccttgaagca gccaagctgg aatgggctga cagcatcaaa
    25561 gaatttgata gccaggtatt aacctttgcg gtggaaaaaa taaaaagaac gcaacaacgc
    25621 cctcccgtta ttcctgtctt tgtagaactt tgcatcagca ttcaaaaaag tattaaggca
    25681 agagaagaag ctctgcgggc taaacccgaa aaccataaaa gaaccgaccc acaaatcgtt
    25741 aaatcccaca tcaaagagat gatggaaaag ctaaccagtc cctcagttaa ggagaaaaaa
    25801 tcatgctgat cctagaccgc aaaattggtg aagaaatcta tatcaataaa ggcaaaatca
    25861 aaatcactgt tctttatgaa aaaaatggcc tgataggaat tggtgtcaga gcatccagcg
    25921 aaatcgatat cgatcgcaaa gaagtcttta tccgcaaata cattcaaaaa ttagaccaag
    25981 aaaacaagtc aaatcaagga tagggatagt tatgttgaga acaaccatta ttggcctttt
    26041 agccctaagc agcctgcaaa tacaagctgc taatatcacc caggttggac gttatgctac
    26101 cgtgaataat caacccctgg cagcacagat aaatccgctt aaaacagtcc agcagattca
    26161 tttcccaagc tcagttcaaa cgataggtga agcggttgag tattggcttc gttattcagg
    26221 ttaccatctt gctccccaga ataagcaaaa cgaaagcctt aaacaaatct tccaacaacc
    26281 cctaccccaa gtcaccagaa accttggtcc tttagctata gccgacgggc tcacggtatt
    26341 ggttgggaaa accctgttta gcctcaagca ggatgacctc ttaagagaaa tcaatttcag
    26401 tcttaatgca aggagagcac aatgaagaaa tttctggatg ccaaatggct tggcctggtt
    26461 gttttaaatg ttctggtgat tgtgtttgtg tgtgtaaaac accaatcttc atccatcaat
    26521 aataacaatc tgaatggtct ggaacaaaaa cttataacta ttcaatcgca attaaataaa
    26581 cctaaccaac aacccgacct ttctcctatt actgacaata tcaaacagct tggggatttc
    26641 atccaacaaa tgcaaaacaa agacgaccat caattaggtg atcttttttc aacccaacaa
    26701 gctgctatta aaaaccagct tgatggcatt agagaattgt tgaagcattt agatagccaa
    26761 aagcagccta ttaaaatgct gccagcaagc gaacttcctt ttagagtttt aagcatagac
    26821 agtgtacagg aagtttcagt cgcatcggtt gcttacgatt ataaaaccca agccctagag
    26881 aaaggggatt ccctagccag atggaaaatc ctagatatca attttgccaa gcaaactatc
    26941 gaatttgaga acgcagataa agcccatgtc ctgatccatt tgacccaaca agaggtgaac
    27001 aatgaataaa ttgtctgcca tcgctctaag cctgttatta ggccagcaag caatcgcggg
    27061 tttgtatatc cctggcattg ccacctcgcc catacagcaa gccaacaatg ccctagcaaa
    27121 agcaggtctt gcagaatttc atgatgaagt catcgaggaa aaggatttcc aacttaatga
    27181 tgatcaaaag caagaagcga aagtctgggg attgaatgaa aaggaagaaa aacgctatct
    27241 gcaattaatg caaagtagaa gcggtgtgta ttataaagac ttgcgcatga cacctattga
    27301 tattcttgga cttaacgcca gagatgatgc ggaacgtgaa cattttgcaa accttgctgc
    27361 tcgccaggaa gcacaaaaag tggcgcaaaa tatcgcttgg aacaatgctt tttataaggc
    27421 ctataacgaa ctatttaaag acgtacccgt ggttggcgat tttgatccct ccccttactc
    27481 accctatgct catcaaccta ttcaattaaa agagggtgaa acactctatt tatttataaa
    27541 acctgacgat gcggttacca ccattgtact gcaactgatt gacgcgatta accgcactcc
    27601 caatacaaaa cttaacctgc tgtttctgga tatgaacaat aacgccatac aactgtgggc
    27661 gaatcgacat caaatcccca ttcaattagt gaccaatcag caaatcacgc taacccctgg
    27721 caaccaacaa tttgaaggct tagcacttgc caagaaacaa accccattac tgttaatagc
    27781 caatggtaaa acctcgcaag ttgtcgattt aggaagattt taatgattag gattctactt
    27841 ttactgtcgg tcgcgttgaa tgttcatagc atgaacgtca ttcctttaac cctgtcacaa
    27901 agtgatacgt cattaaaatt aaatttgtcc agcccaatag gcgtaccagc caaaagcaaa
    27961 gccaccgcag gcaaagtcag ccgcaaaaca ttggatacca ctttattaaa catacctttg
    28021 tttgtcatag gtgcagataa agcatcgatt caatggttgg aacagcatca agaagaatta
    28081 aaattcatgc aagccacagg attcattacc aacgtcaatg actttgaaac cattgtcgct
    28141 ttgcaagaaa aattcaaact accgttactg cctgtcaatg ttgacccatt gcttgcatac
    28201 cttcatgaac aacattaccc cttaatcatt gctgaggggg ctgtatggca gtaaagcaat
    28261 attcaaggca actgaccatg caattaacca tagtgctatt cttaggttgg ttagcaatta
    28321 ttatctgggc attgagccaa tggatgctgc atggctaccc catcgctttt gaaaaagtga
    28381 atatccttgt aaacactcaa agagaggcta ttagtcctgt ttatcaaggc tcccttctgg
    28441 cagtccttcc tttaaatgcc aaaccagatt gggcattagc cattccttac gtaaacaaat
    28501 tggtcattaa tgatcttgcc aaggatttaa tcgaaaaatc acaacaattc ttgcaaatcc
    28561 ttttattaag cagccagtgc cttttaatca aactcattat cttattcgct gccctgccct
    28621 tatttgcttt aaccatgatt gcaggtctgg ttgacggact aaatcaaaga gcgatacgca
    28681 ctgcttgttt aggccgtgaa tcctcttatg tgtttcacag gcttaatcac tatttaaaaa
    28741 agggtcttgt gattttactt atgctgtggc ttgccctgcc cgtcagcatc acccctgctt
    28801 atgtttttgt tcctatcagt ttattgatgg ggttaatggt agcgatgacg gccagccgtt
    28861 ttaagaagta tttataggga gaaaccatga aacgattgct ttgtttgacc ttgataatga
    28921 taagccttca atgctcagca aataaccctg atttaaatga aactttggtg cgcatcatta
    28981 atcagattaa cgccatcatg cctttgcttg atgaagcgca aacacaagta acacctaatg
    29041 ctcgtatcca attgcaaata gaaggatttg aagacctcca aggccagcat cactccggat
    29101 tgagagaaga cttgctaaat atccgcaacg gcctgattga ctacatcaac cagcccatca
    29161 ttgcccctcg aaaaattaaa ccattagaaa tggattttgt gaggaaacct taatgagtaa
    29221 agacgaaata gcgagaatgt ttgcttcaac attcaaacaa gccagcaaca acatctcagc
    29281 ccaagtcttt tccagcagta tccgttttat tgccataagc ctggtgattt tagcggtgat
    29341 gtggtgcgtc aaccacatga tgggaaccga ggaaaaacag caacaaggat acttgctgaa
    29401 cttcggctct cgaatggtca ggctggtcat tggcctgatg attttcattt tagtgttatt
    29461 cgttaaggag acaacatgaa aacaacgctt cgcacgctct acctgggagc tgtcaatctt
    29521 ttttacgccc ctgtgttatt ggcagataac tggttcccta aaatttctga tgcggacaac
    29581 atcggtgacg gttccaaaag catgatgtcg gtaacaggca attacgtcaa acagggtctt
    29641 ggcttattgt tgttcatcac cgcagtcgtg acctttatca agttcattac cacagtacaa
    29701 cacggtattg aagaatccaa aaagaacgaa ggttccatgg tggccttcgg tacttttgcc
    29761 gtaatgggaa tcacctattt agccattagc atcgcttctg gttacgtggg ttacaccatg
    29821 attaccaagt tcaaaatata ggaggtgaaa ttatgagtca gccttcttcg cgccatctat
    29881 cacatgactt tccagcctgg aaaggcttaa gtctgcgtga attgttttgg attgtcatca
    29941 ctacaacacc agtaactacc ttgcttttta ttcttttagg aacaatggca gggtatccac
    30001 tagccgctgg ctgcatcggt tttgtgattg gattcatctt ctccattacc gtatggccaa
    30061 aaggagtcgc ccgtatcaaa gcaggcaaac cttatggtta tgtgatgaag aaaaccattc
    30121 aatggatggt gcgattaagg ctcaaacact cgccctggat tcattatcag gggcagtggc
    30181 aaaagaataa atctgttgga gagcctcatg tttgaatatt ggaaaaaaat cgacagccta
    30241 cagcatcata accgtttatt actggctttt atcggcctgt tatcagcgat cgttttggcc
    30301 ttaatcatta acctcatgac catgcccaaa cgctatgagt tttggctaag ccctaatatg
    30361 gcggccaatg gcggtttaat gaaagcaagc gatatcccta atgaatatgt gcaaggattt
    30421 gtgtcctcat taatacccac gctatacacc tggtctaata aaggcaaaga agaatttaac
    30481 caaaatatca aagcctttca ttattatttt acgcctcgtc atgaacaatt actcagagaa
    30541 acgctgggag cttataaaaa cgcgcagctc tttgaccgca gtcaagtagc cagtctctat
    30601 cgtttcatgg agcctcaaga tattagaaaa attggtcctg atacctggga ggtgaaatta
    30661 atcctgcgca ttactcaaag actgaaagac aacagcggta tggtgattgc cgacaaggta
    30721 gtggcctatc acctacgcgt ggtcaaactc aagttgtctc gtttacacaa ccctttccaa
    30781 ctcgctctcg atggctacac ccgtcctgaa gaacgtttac aagatttatt ggtcgacacg
    30841 gaggaatccc atgaaactca ttaaacacat attattaata attggcttta tgctcacgca
    30901 agtaagctac gcgctgcaaa gtgaacatct ggtttgggaa aaagtccctt taacgattga
    30961 attacctatc aataaagaaa ggctcgttca atttccccag gccattaaaa tcattgatca
    31021 acaactcagt accaacttag atattctgaa agtaaaaggc agtctgtatt taaaggccaa
    31081 agacaccttc aacgatgcac gtttgattgt acaactctta ccagaaggcg aagtgctcat
    31141 actgaatctt aaagcaaatg agaaagccgc caataccacg cctattgaaa tcctgataga
    31201 taatcccaaa accactcatc agtcaggtgc aagccaatac gaatacaacg ccatccagct
    31261 tacccgcttt gctattcaag ccttgtattc acctgaacgt gtcagaaata ttccagaggg
    31321 tatttatcgc tcgcccatgc aaaccaacaa aaccattccc ttattttatg gtgcaagcat
    31381 tgaagcgcac cctctggctt catggcgcgg cggtagcctt tatgtgactg cgatcgattt
    31441 gaaaaacctg ttaaatcaac ccgtgaaact gcaattttca aaactcatgg ggcattggca
    31501 aactgcaagt ttctatccca aaagtgaatt gcctccgcga aatcaacacg aaagtacaac
    31561 tgtctttgtg gtttctgacc aaccctttgc cgcagcactt actcaacatg cgaggtacag
    31621 ccgatgaaca aaaacaaagg cattaaaatc ttggctggat ctgtggtgtt tgcggtcgtc
    31681 atgattctta tcaatagccg tcatagtacg cctatccatg atgacacacc taatcccaac
    31741 aattttcatg aagccatcac cagccaattc aacgacaaca tccgtgatgt gtctgcaaga
    31801 ctccttgaaa cagaaaagaa gctggaacaa atgcacaagg agaataaatc gctgcaaaaa
    31861 caattaaagc aaccgataca agaagcaagc catacgctac aagatgaatt gcaaacgctg
    31921 aaagaaaagc tctcagcact taccgatcat caaactcaat cttatcccat ggaaggcact
    31981 caagctcctg tatctggcca actcaaagat ctggatggac tgctggataa agcccctatt
    32041 gaaggaaaaa tatttgcaga ccaagactca tccaccaaag aaaaagcgcc aaccaaaaca
    32101 cccttctata ccattccggc aggtagtgat tttagcaagg ttacactttt gtcttcccta
    32161 atgggtgaag tacctgttga gggcaaactg atgcagcctt tatttccttt taccgcaatg
    32221 gtcagccgtg gtgatttaat gactgccaat gggatgcaat taccggaaga aattatcggt
    32281 atgaaaatca gcggttattc cattggcgta ggctctttcc ttgataacat cagctgcgtc
    32341 cgtgcttatg tcacctcagc tttatttgtc tttgaagatg gacatttcgt cagtgctggt
    32401 caggaacaaa tgaaaagctc tgcggagctg gtgaacaacg acagcattgg ttatctaact
    32461 actcaattcg gcaatccttg cattaaagga cagtacatca ctaatgctcc cagagtttta
    32521 gctgctttta tggctgctgg aggtgttcaa ggggcaggca atgctttatc caaatggcaa
    32581 atgagttatc aagccgaagg cggttctgct attgccacgc ctactggtga tttagcccat
    32641 ttcgcagcag gtggagccat caacgagggc agccaaaaaa ttactgattg gcttgagaaa
    32701 agaatacaag gcagcttcga tatggttttt gttccagcca gcatccgcag ccactcaggc
    32761 tttataccaa atcaattcag tcttcatttc agtcaaacca tagccattga taaagaacac
    32821 caagggaggg tattagatta tggttacaat caacaaaaga actttgacaa tgctttgcgc
    32881 tagtctggct ttgagcgctt gttcaaccgc caatatatcg caaagcgcca ttcctgagca
    32941 gggtttaacc gtcagccagc tctatcagca aagcatgcag cagcctgtac aaaaggctgt
    33001 tgtacaacag gtacggtttg agaattcagg cgaagaattt aagcagctgc cgaatcccga
    33061 agtgcctatc catatttttg ctcatgtggc tcagcttgac gatgaacaaa tcatcaaacc
    33121 agcctatacc acccgctttt ttctttataa gcaaaatcag tatgccctgg catcagagct
    33181 gtattaagga ggcgtgatga aacgattatc taactggttg cttggggaaa tgaatcccca
    33241 ggctataaaa gaactaacca ttaaaaagca atacgccaat cctttgccct cgtttgctga
    33301 caggctggcc attgttgatt tcaacgaaca agaacaagtg tttttatttg ctgatggaaa
    33361 aagtttaggt tcagggtttg agctaggtga tattcaagcc gaagcagctt ctcctgaata
    33421 cttgcacgct gtctttaata aaatccgcga tacctttgca acagtggttc cactacatgc
    33481 aatagaccct tgggttatgc aaatgtttgt tcaagacgaa tattgccttg atccggttat
    33541 cgatcatatt aaagcccaaa ttccacaaca gtttattgaa agctcattaa cgcaagatta
    33601 cttacaacgc ctgcaagatc tctacaacaa aatgaccagg ccagaaggtt tatttctcga
    33661 tcctaaaaca ggtgcgccct atcgaggacg aagacgacga atcagagtat tgttttatag
    33721 acaactacat caaaccactt taaccagaga acaaattctt ctcgaacatc aagaagtcat
    33781 cagtcagatt gaaaccaaac tacgctcacc gggtttagaa atcaaacgcc ttaaaggtca
    33841 agattattat cagtggtgga tacgctggtt taatcctaaa tcagccgatg agatcttgga
    33901 acaatatccc taccctaatc acatacccgc aggttttaat ctggcacaaa atatattctt
    33961 ttcaccgcct gagagtgacg aacaaggatt tatttttgaa ggccgaaaac aaagaatcct
    34021 gtatgttgat ggcttaaaag aagcacccat tattggttta gtctcccgag aaagacaaca
    34081 agccaatcct aagcaccgat atgcgctttt agatactttg ccagaaggaa gcatctacac
    34141 tattcaagtc gtctttagcc atgatgaaaa tcttgattcc catttaacac gattagaaaa
    34201 gggaattgtg ggaaccagcc taaaacccca agaggtcaga gatgatataa aaacagcccg
    34261 taatgaatta agtattggca atcgtttatt ctgggttaat caggccgttt tttatcaagc
    34321 agaggatgaa aaacaagcac aagccataga aaaaaacctg cataccattt tcaacgatgc
    34381 aaagatgccc ttaattcatt ccagctatga tatccaccct ttaaattcct ggctcaatgc
    34441 cttgccgttt aatttcgatc cccgctatgc ccgtaagtac ttatgctttg accgtctgat
    34501 gtatgccacg gaacttgcag cattgcttcc agtttatggc cgaaaccaag gggcaaggca
    34561 tctaccctgc tttccttttt tcaaccgctt aggcgaacct gtattctttg acttgttaca
    34621 tcatgatttt attagccaga attcacattg cgccatcttc gctaactctg gtggtggtaa
    34681 gtcagtttta acaggttgga tgattcaatc attactcgcc atgaaaaacg cccgtgtagt
    34741 actttttgaa atggggaact cgtttgatcg gatgctcgtt catgccaaag cccatggtaa
    34801 gaaaaccaag caattattat taagcaataa aaaagaagaa gctgttccat taaatccatt
    34861 ttgtgaagcg tataaagcaa ttccagaaat aacagataat ttaagcgcag aacaagctgc
    34921 tttaatagct caaaaaatca tggatcttaa agatcctgct cactcagaag atttagcttg
    34981 tggtgttggt tctcgtgcat accttgcaga gcttgcttta gctttgcgta ctatgattac
    35041 cgaagccaat tccatggaag aagaacaatt taccctagct gatgaaacgc tgctaatcga
    35101 agtattaatt gatgccattc taacgtcatt taacgagggc ataccacaaa tgctgacaga
    35161 gcatattgtg agtgcatttc aacggcgatt agataaagaa actgtgacgc gcaagaaaga
    35221 tcggattctg gatatgcacg accgattaaa cagttatgtc attaacagcg ccaaatcacg
    35281 cttttttaat gtaccaacag aacctttagg tgattttgat atctttcata ttgatatttc
    35341 tgccattaaa gatgataccg gaaaactggc tttggtcatg gtgtctttgt tgccaagaat
    35401 actggctatg gcagaagcca ctcaaaacga caataggcca acctttcttt ttattgatga
    35461 atcccatctg caatttcaaa ttccttctgt ggtgacagtg tgtctactgg tagccaaagt
    35521 agctagaaaa ctcgggcttt ggctggttgc aattacccaa aacgttaccg acatgaattc
    35581 tgaaaaggct gcaaagattt tatcgctaat tgaaacctgg attttgttgg gactggatga
    35641 aaaagaaatc actgatgtga aacgctttaa atctttaaca ccgcagcaag aaacattaat
    35701 cagagatatt gattcgcaaa aaggtttgta tgcagaagca gttttattag ggtcacgcta
    35761 ccaaggatta tttcgcgtca ttccacctcg ctacctgctt gccctcttaa tgactgagaa
    35821 atctgaaaaa gcacaacgcc accttctgga gaaagaacat gatgtccttg aagcagctga
    35881 aatcatggct aaaaagctcg aacaaccaaa aacaactact aataacaggg cttattttta
    35941 tgactaatac tgcctttgct ttagaaagca caagcccgcc tcatcctgtg aataccttca
    36001 caatcgccac acgggttttg caaaagctgt ttcataacag ccattacaaa gtcattggct
    36061 cttgcacttg gacggtggga cgtttaccgc caagacttga agttacccct gcgattgaac
    36121 agtttttgcc tgatctagtg attactgtca gcaataaacc agaagaaaat ccctggattg
    36181 aagcacgaac gctttatgaa aataaagcag caagaacgat gtaccaacaa gcctataaag
    36241 ctgcgactgg ttcagcatta ggattcggca atgacagcgg tcaaaccaca gatcttcaca
    36301 tcaatgatga gcgtactcga attgttgatg tgattggcag ccctgctgcg ttttaccgta
    36361 tcccctatct atcacacaaa ccagaaactg gatttggtgt tccttattat ctcaccgaag
    36421 ctgatgcagt catggataga acagaagcag ctgaattgtt gtatatggga actcatcctc
    36481 acctgttaat taatcatgaa ataggcacat tcactcagca ttggggttct gaaattccta
    36541 ggttaatgcg cgtcactcag ccttacaact atagagcctc agtcgttgca gccatgcacg
    36601 ctgtagatat tgtgactaat aaaaaccctt tacatgtcac taagtcaacc gctaattcct
    36661 gcggtaaaaa ctgtgtggtg tctaatgcta tttacgaccc acaaaacacg aaagtcatct
    36721 ggcaggaaat ctatccgtta aatcgcaata tccacccagg tgacgcacag gatttcggca
    36781 ttgaagacga aaaagccgga aatggcaatt acgtgtttat tctttggcgc aaataccgtg
    36841 gctgtattca gcataaaggc aaactcgtga actttcttac ttttcccaag gttgggcaac
    36901 ctcaaaaacg ataggacatc atcatgaaaa aatatctatt actagcttta ctcgttccaa
    36961 cactgactca tgctggcagc tttatgccta gccaatcgga ttactattat gaattaggcg
    37021 gaacctccaa tttatttatt ccacctgtta ataaagacca aacgctggtt attggtgcag
    37081 atattgatgg ccgtatggga ttctcctgta gcggttttaa tcctgtcgta tccattacca
    37141 atacttttca agatttgaaa aaatcagcgt taaacatccc gggtggtgtc attgataact
    37201 taaaaggttc agtcgcagga ttccctcttt ataagctaca acagtccatg cctgctttgt
    37261 acaatgttct acaaaatgcc gcatccagcg cgcaaaatga atttaccctc aaagttaaag
    37321 attgtcagga agtcaaacaa gcattggaac aaaaccaatc accaatggaa ggtatattat
    37381 cagtctctga tagtcaggga tggctcgatg ctgccaaacg agctaagaag gaaaacgtcg
    37441 atgtgactga aacatcgaaa agtattgctc aaaaacgaga tgaatatggt ctgccctgga
    37501 taggtagcga taaaggtaat gctgggggta aatttcaacg tcccattaag gtcatcaatg
    37561 atgtggttat tgctggctac aacatccttt tagagagaaa acccttagat tctttggaaa
    37621 agccaagtac caaaatccct atggtacaaa gctggccaac acctaccgat gctgctaatt
    37681 gggcagttaa ggttcttggc gatattcagg tcagcagcag tgagaacaaa caaagccatg
    37741 atgctaaagc aggtattggt ttatcagctc tattgcaaag ctgtgcgaac gccaatacct
    37801 gtagtcagaa cattgctaaa gccttatgga atttggtcaa tggtacttgg acgctgaccg
    37861 aagaaaatct gagaaaagtc agcgcttcca atttgctgat tactgatgaa gtcattacaa
    37921 ccattcagca tctacctcgt gaagaacaga tgctcaccgt ttcaaaacta gcagaagaaa
    37981 tcgctgttca aaatatgctg gataaagcgt tgatgatgcg ccgcatcttg caggcaggat
    38041 tacaagtgca agaagtacag aatttaaaac cagctatgaa tatggtcttg tttgccctaa
    38101 aaaaattgga tgatgacatc cactctttgt cctttgaaaa cgatgtccgt aaaaaaatga
    38161 tgactgaaac cgtgggaacc atcatggata ttcgacatca agcacaaagc caaaacatcc
    38221 caggccagga tcacgaacaa cctgctgtta aaaacggtgc aatctatgtt aaagaaaata
    38281 aaggagctta atcatgattg tctttaatcc tttatctctc tataccacct atcttggttg
    38341 gcaacagtat gaggttctat ttaacgccct gtggcaaaca gggctgttat atcttggttt
    38401 tttggccatt ggttatcgct ttcttaagaa cgtgcttaat ccagctggcg cgttctatgc
    38461 ggttgaacat gctttaaata acttccttta tgaattggca gttactttcc taatatgcag
    38521 cttattcgtc tatccctgtg tgccgctaga aaccaaagcc ctgcaattta aacccttatg
    38581 tggattgaag aatccaacaa ctgcggtaat tggcgacagc ggaacaactt atgatgaagc
    38641 ctttgcagat ttactaacta accaggtcaa gattcctata ggctttgcca tcatccagaa
    38701 cttcatgtcg agctttactt acagtctcat gaaggtaacg ggttgcaccg acagtttgca
    38761 atccattcaa ggcgatttgg tatcaaccta tctgccacag aatattcgca aacaagctct
    38821 ggattttcat agacaatgtt ttattgaagc aagaacgaaa tttaatagtg aaaagcatga
    38881 agccagtgaa ttagacccca tgctgaagcg ctatggtggt gaggatgatt taaactggat
    38941 gggttctaaa atcctgcaaa aaatgtatta cagcaaactc catgcacgcc aacctgttcc
    39001 tggttttaca ttccaccaag cccctaatcg caatcttgaa aaagcggcca accgtggcga
    39061 tattccacca gaacaactgc cagaagacgg ttatcccagc tgtcaacaat ggtggcataa
    39121 gatcaaagca gatttggttg atgtttctaa ccaagccagt gtttttaata agcacctaaa
    39181 ttactacgcc atgctagata gggttcgtaa gtataaaatg aaacatccta aagcctggaa
    39241 agcagacatc agcgcagaag attttattgc caagatgctc ttgcaagaaa gcaaagatat
    39301 gcaggcaagc tcagtacaaa atctcatgga caataacaat ggaaaggtcg catccgcgat
    39361 tacccatagc ttggtcaatg tcggacaatg gacaaaatca tggacatcta ccccattaaa
    39421 aagagaagcc atcatgcaaa ccttacctgt aatgcaggcg tttttcatgt tctttttaat
    39481 cattctcacc ccaatggttt tgtctttgag ctgctacagc accagagcat taggcagcct
    39541 gtgcgcctta tttgtcatgg ctatttttct tcaatacctc tggcatttag ttggatttct
    39601 tgaacgttca gtacttgatc cattaggtga aaacgatgcg atttctgcca tgaaaaatat
    39661 ggctgtgatg ttttactttg tcgcaccagt tttattgctg agattatcaa gtcattttgg
    39721 cggtgacgct ggggctggtc ttatggattt ggttaatggt tcggataggc agagcgaaag
    39781 catggctcaa tccggtatgc acgtagccaa gacaggagta aaaattattg caaaaggaat
    39841 tcgatgaata tgttaaagca catccttact ttcaaaggct tcattgtatt cacgaatagt
    39901 gatcaagcca ttatcaagtt tatcttgagc ctcataaaca gacatgtagc gtttgtcagg
    39961 tttatcctct gccactccaa caatggcatc aagaacaacg gccaacaacc gaaataccct
    40021 tctcatttgg agttatccct atgattaaac attatatccg ttcatccatt atagtattaa
    40081 ttcaaaccgt tttaccaatg attgccttat tagtatttgc gccctggttc attaatagca
    40141 atctactggc acgctggcaa agcaccttca ctaccattca accattattt ctcgccctgc
    40201 atggagtgct ctatttagcc cttgtcctat tatggccaaa actaatttca cgcctacaga
    40261 atcaacacca acttaccgca gagcgactga gaaccgccct taacgctcgc tggtatctac
    40321 tggctattat tgtattcatt gatgctttaa tgattgggag tcgactatga gcaattaccc
    40381 tattaataac ctattaagag aaccaacaga agcctggtca gccattacct gtttatctct
    40441 ctccttactg gcatatactc aaccgcattt gtttttacta acacaaacca tgggattata
    40501 tgctgcactc gcattattaa tccctggaat ttatagagcc tggcaagcaa ttaaagtaaa
    40561 acgctatcac cgaagattat tagccatgcc cacctatgcc ctatcgacca ccgaaatccc
    40621 tttatccaaa aactggttgt tccttggcaa aggctttcgc tggcgaccaa gccacactca
    40681 aaagctacat caaattaaac aagtcaaaaa tgaaaagttc atgcaacgaa actattgcta
    40741 ccagcttgcc agaagattct gccaaaacca tgaacacacg aaattaacca aatggctaaa
    40801 taccaattca aaactaaatc cttttagacc tgatccacct gttggcggta gcccgttctt
    40861 gcatggcgta ggggaagaag ataaacccgt atacatccct caagatgtac gtgtaggtca
    40921 taccttcgta gtcggtacaa ccagggtagg aaaaacacgg ttggccagca ttctaattaa
    40981 ccaagacata cgaaatggcg atgctgttat cgttgtagat cctaaaggcg atcaagacct
    41041 ggttagagat atgatggcag cctgtaaagt atcaggacgg acagaagatt tcaaaatcgt
    41101 acacttaggt tttccagagc agtccgcaca atacaacccc ttaaagaatt tcgaccaaat
    41161 cagtgaggtt gccacacgca tcactgatgc aatctcagca gagggagaag gaaaacagtt
    41221 cgcagccttc gcctggaagt acgtcaatat cgttgctata tgcctggaag aaatgaatca
    41281 acccattacc taccaaacaa ttgccttcta tatcagccga ttagaacagc tgttaatgtc
    41341 ctatgctgac aacattcttc ctaaccacta tcataattat catcaagaga tagatgaaat
    41401 catcaaggaa catgatagta aaataggaaa aaataataag acaaaatctc caatggagcg
    41461 tcatcaggca gtcgttcaat atgtcaaaga tcacataaac aaaaccatca agagcaacaa
    41521 tgctgaagct ttgcacgatc aaatactgat cgacctctat gatgcagcca ttatggacaa
    41581 acactattac gacaaaatca ccgccagtgt tggtcctgta ctttctgaaa tcaataaaag
    41641 caatgcctca aaaatcttat cgtcaaaagc tcaaccagat gaaattgaac tgatggatgt
    41701 aattaaaaac aagaaggtgc tttatattgg cttagacagc ctaacaaatc ccaatattgc
    41761 ccaagcagta ggaaaagcct ttttgtctga tctggtgtct acagctggta aaatctataa
    41821 agaacccaac gcacgataca ctttaaatct acactgcgat gagttgtcag agattatcca
    41881 ggattcattt gtgaagattc ttaataaagc tggcggtgct ggatttcagg tcactgctta
    41941 tgctcaaaca attcaagata tggaagttgc acttggttcc agagctaaag cagaagtatc
    42001 tgaaggaaat tttaataccc tgattatgct gcgtgttaaa aatgaagaaa cagccaattt
    42061 actggttaaa gtactgcctc aagttggtgt tgtcggacat acacaggttt ctatggttaa
    42121 cgatacgcca catggggatg atagggttta ttttaatacg actaatgagg atcgggttca
    42181 aacttcttca gtgcctatga ttggggtgga tgatattgtt tcattaccta aaggccaggc
    42241 ttatttatta atgtctggtt gtgaaattta taaagttaga attccattac ccaaccatta
    42301 gattaatttg gattttagaa caaattcgac catactatac tacgtggcgg tatgaattaa
    42361 atttccattg tctatctgcg atggttataa gtatgttatc tattacgctc agtattatta
    42421 ctaaactgtt attgcagcta agggtataag aaaaattcta ccgccaatta tatggagtgg
    42481 ggatggagca aacaattaat aaaaatttca attccttaga tacactggta agtttatttg
    42541 gtattaacta ctctaattta gcaaagttaa tttaccccac tacaaataac ttatatcaca
    42601 gtttttcaat tcccaaaaaa tcaggtgagt taagagaaat a
//
LOCUS       DATNSN010000010        40809 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.010, whole genome shotgun sequence.
ACCESSION   DATNSN010000010 DATNSN010000000
VERSION     DATNSN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 40809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40809
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.010"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            61..1086
                     /locus_tag="VAH76_000442"
     CDS             61..1086
                     /locus_tag="VAH76_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385095.1"
                     /translation="MGWTDRFWNAYRVVTNTVIEGAANSLNAAGSLACLLGGAGVSLS
                     YSMSETIKAGYFGSVGVAGTVNLDVTAVEFNYGFNQTIPFRRDLLEKSGGGSYALNDF
                     ANPGTIFMISSLAILSGTTLRTFGANLKKWQQNREDKYHFIAKYSTDLEPPSYKEYLY
                     VNAESFFNSLAIASFSSLLAKNVLDFSIKDLSKYHFTYPFNGTNITGSVKTPDYTGPV
                     TSEFFPLDFFVSQNTTIHTDYFNLIVNEKVKANAMTNVTYGGGVHLEPSHQSSAPLLA
                     ASATIGIGTYLAGTFFGKKILRERDERLQRSRSSSYSIVSGTFWDNKHDIEAKGDTKF
                     EHTHSLQ"
     gene            1148..2341
                     /locus_tag="VAH76_000443"
     CDS             1148..2341
                     /locus_tag="VAH76_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO1385096.1"
                     /translation="MVFKSRLVGVILLCFSSITGWCLSQSPEEDRIIVSNCLYEQLKD
                     KARLIAQSDHFSFIAIPIDENITNTLQKMRPVCGNYLNAEPYIQQTSNRFLDSQKLLD
                     KLTSYHSISTANPYPINHEAQVHGLLEQIDPAKIWQTNQHLTSYINRSAKSRTGVEAA
                     QWFKQQFDTLAQDYGRKDVESYFVKTGNKFIQPSVVTVIGKDKPGEAIVIGAHIDTLD
                     GNMPGADDDSSGISVELEMARVLFSSNFELNRPVYFIAYAAEERGLIGSGYVVQDFLQ
                     KKIPVKAVMQLDQAGYRANAKDQTIWLLKDYVDKGLTEFTAELLTRYVKIPVGYTKCG
                     YACSDHVNWTNEGFKTTYPSATTLDDDNPYVHTSNDTLDILNLEHMVNFTKLGLAFIV
                     ELGLN"
     gene            2400..3290
                     /locus_tag="VAH76_000444"
     CDS             2400..3290
                     /locus_tag="VAH76_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385097.1"
                     /translation="MKTEALLSWLNVESNESYDSIRIKYQLRLEALEKEERIKKMGGI
                     NPFAPKIPLNNTRIITSKEIQEAWAAIDSLSKYQQFKGLVAQEENLSTIPNKKMDSKR
                     EETKLIHFVRERAQGPQGRRLPAHLQAKIKRAPLDKPHSKLITEGVEQQINAILDKTT
                     LSASDLQSAVYTAVQNYTNYHMKSENKNNPKYNRGQGDGFFSFFRHGSFGITTANNLY
                     TSISAKNATLDETIEKLKDFLGDSSRAYHHHSFTSYLADALAEKGLLTSHPASRYKQE
                     DVVNQLDQWIAQNSTVNLLK"
     gene            complement(3390..6320)
                     /locus_tag="VAH76_000445"
     CDS             complement(3390..6320)
                     /locus_tag="VAH76_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1385098.1"
                     /translation="MIDKMRKSKKPHPLNHIASGDNFSHLNLLERSLISQLIELSYDE
                     NTLHTLSLSGLELLGKHPLLKDTITAVWHHDGSHINILASNGLKFDAPKELEKFLAVV
                     SDWHFLKIYKTKLKKHSEEKLKQRIDICLDKCQGNLFCISFWFDSIDCNFEILMPFFK
                     DILGILNLSLAKLMNKKREKLLTTILEKAGDSVEITNEEAVIQYVNPAFENITLYNAR
                     EAINKTVASLLRSPVEDAQLFEEINQTLKSGNTWKGQIRSRKKDGSYWVAQTIIVPVM
                     DDELGRITQHIAIKQDITEQVHRFNQLKISEERYRNLMNAVADGIFIHNLQGAFLEVN
                     EAACLSLNYSREELINSYVWDIEVGFSQESLNQLWENVQKGPINTEGMHRRKDGTIFP
                     VDVRLAVFNATGEKLVLAIVRDITLQKRSEATIRKLTRALEQSPVLVIITDKTGTIEY
                     VNAKVIEQTGYRKEELVGGNTRILRSGQTPHEIYKTMWEHLANGEEWHGELLNKNKSG
                     EHFWVSAIISPIRNENEEITHYLAVMEDVTQKKSYEEMLKHQATFDNLTNLPNRLYAV
                     NKLERAISKSHINNQKLAVLFLDLDEFKQINDSLGHAIGDLLLKALSERFLSMIRQTD
                     TIARLGGDEFMMILENIMQDKDAESIAKKCREICSQPFVIESQELLVSTSIGIAIYPD
                     HGKDAKTLMRNADTAMYQSKIRGKNNWTVFQKTMIDGANNHIRIKTELHQAFTRNELY
                     ICYQPIIDLKENKVVAAEALLRWQNTTLGQINPDQIISLAEETGMIIPLGYWILDKVC
                     TQAKEWEFITGSKIKVAVNISTLQLKQKDFIHKVQLILKRTKLSADSLIFEVTESAFI
                     DDSKFILSQLNKLSDIGIHFSLDDFGVGYSFLQYIKSYPFKNLKIDRVFIQGVNTNHN
                     QLSLINSIITMARNLKLSVIAEGIETAEQLELMRSMNCDMVQGWYFSKPLSGDKFLFF
                     LMNK"
     gene            6543..7184
                     /gene="coq7"
                     /locus_tag="VAH76_000446"
     CDS             6543..7184
                     /gene="coq7"
                     /locus_tag="VAH76_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEO1385099.1"
                     /translation="MRTSSFLDRLIGEVDSALRTLVLPQKRITTRQSPAENLADTVLS
                     AQEKKHISGLMRVNHAGEVCAQALYQGQALTARLTHIKEQMASAAAEEVDHLAWCEER
                     LYELGSKPSLLNPIWYCGSVLLGALAGLAGDKISLGFVAETERQVTAHLQRHLHYLPE
                     KDKKTIAILKRMQEDEEHHAHTAMEAGAVELPYIIKQLMNAVSKLMTQSSYYI"
     gene            7201..8196
                     /locus_tag="VAH76_000447"
     CDS             7201..8196
                     /locus_tag="VAH76_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEO1385100.1"
                     /translation="MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQ
                     AVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGL
                     PSSSSHALIGGLAGAAIAKGGLSSLKIAGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
                     LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPF
                     WVVISCHGVISLGTLAGGWRIVHTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPV
                     STTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALVAAAIMTISPTA"
     gene            complement(8206..9597)
                     /locus_tag="VAH76_000448"
     CDS             complement(8206..9597)
                     /locus_tag="VAH76_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1385101.1"
                     /translation="MDLFRKKDIKGSLDNESHLAKCLSVFDLTFLGVGAIIGAGIFVL
                     TGIVAATISGPAVIFSYVVAGFACAFAALSYAELAASIGGCGSAYGYAYAGFGELIAW
                     IVGWDLLLEYSIAVSAVSIGWSGYANDFLMALKIFIPANLLHGPADGGDLNILAILII
                     AVLTTLLIVGVKSSSRFNNIMVLVKLLVIFIFIAVAFGEVKVENWSSFMPYGWAGVMK
                     GASIIFFAYVGFDAVSTAAEEAINPQRDLPIGIIASLAICTVIYILVSGLLTGIAHYT
                     TLNVSSPISHVLLVLGYKTAAGLVGVGAIAGLTTVMLVLFYGLTRVFLAMSRDGLLPR
                     FLSKTNEHTKTPIRIILLCGILMASLASVTPIGTLAELVNIGTLFAFIVVCGGVLYLR
                     RTHPDMERPFKTPFMPYVPILGIISCFYLILNLPWVTILRFIIWMLVGIAVYFAFSRT
                     NSALSVEGDCAAE"
     gene            9757..10329
                     /gene="pagP"
                     /locus_tag="VAH76_000449"
     CDS             9757..10329
                     /gene="pagP"
                     /locus_tag="VAH76_000449"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HEO1385102.1"
                     /translation="MKRLISCLTIICALNASAAAETTSNPCSRWISFLKPVCQRIHQT
                     WAEGHDDMYFSGYAWHNRYVYSNEKIKSYNETAWGGGLGKSLFDEKGNWHGLYAIAFL
                     DSHRHLEPAVGYAYLKTASVNKDLKAGLGYSVLVTSRVDYDNVPIPGALPWAALFYKR
                     ITIAATYIPGSSREGHENGNVLYMLGKISL"
     gene            10469..10894
                     /locus_tag="VAH76_000450"
     CDS             10469..10894
                     /locus_tag="VAH76_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HEO1385103.1"
                     /translation="MMLKTQLTAFIGAVILAGSSLANPIKPEVCPSVPSIQSEGMSMS
                     SEILEGMYITYNLSHYNTSSSWVFIVGPIAAENDDMALAESNKLLSTMPGSPHPEDDG
                     EGNWICQYTTKSKDIIAFAIEADDMLSPLKMMRYLRTIR"
     gene            11041..11583
                     /locus_tag="VAH76_000451"
     CDS             11041..11583
                     /locus_tag="VAH76_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEO1385104.1"
                     /translation="MKSFIEQAQFYATYHQNPATRYTHMAGVPLIMLSIMIFLGFVKI
                     VIPGVYATDLACLATLAALIYYFLLNWQLALALTPILIFLLWLASWFNYDGPTKFGLW
                     VFIITFVVGWGLQFYGHFIEGKKPAFMVNFTQALIAPLYLTAELFFMAGLMNSLREQI
                     YGNEEVNTEEISKTKTKSKS"
     gene            complement(11653..13776)
                     /gene="rlmKL"
                     /locus_tag="VAH76_000452"
     CDS             complement(11653..13776)
                     /gene="rlmKL"
                     /locus_tag="VAH76_000452"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEO1385105.1"
                     /translation="MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPV
                     IYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQ
                     IRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
                     HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAH
                     IAPGLLRQDQSLQYWARHQSSLWEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNA
                     ERAGVLPLVEFNTLPLNACRPGTKKGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTC
                     YQGWQAAILTSSPVLAKALGLRADKHYTLYNGPLECKLYCLTLSAANKLKNTPNAPLS
                     DNAQMLFNRLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPP
                     ASIPVHKAEKRSLEMLQVVPRALGIHPEKLIVKQRKQQKGSEQYQKIGKTSQRLIVTE
                     GKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAALAGALT
                     TNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDCKEWMKITRDKFDVIFLDPPSFSNS
                     KRMSDILDIQRDHVSLINMAMRLLNPNGVLYFSTNLRQFKLEPMLKEKYAVQDITPQT
                     IDQDFKRNSKIHHCFKIVMPHFADN"
     gene            complement(13773..14252)
                     /locus_tag="VAH76_000453"
     CDS             complement(13773..14252)
                     /locus_tag="VAH76_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEO1385106.1"
                     /translation="MDEQISKSQKKRDAHYLQDIGIKFIDLSASKLDSLPLPDNLRQA
                     IIDARSIKSHGAKRRQAQLIGKLMRAADFEAILAAYEKILEEESSITASFHEIELWRD
                     RLLQDGKDALTEFIDAYQPEDVQQLRQLIKKALDDQMKEKNTGAAKALFRYLRSLIK"
     gene            complement(14444..15355)
                     /locus_tag="VAH76_000454"
     CDS             complement(14444..15355)
                     /locus_tag="VAH76_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO1385107.1"
                     /translation="MAFFLITLCILILSFFCLAVMVLKFMQQPSSPLSFSQYVKLMCS
                     GVIAFIADTVGVGSFAVNVALAKLLGTFRDDELPAVNNGAQVLPGAIESLFFIHMVDV
                     DLTTLLTLVTGTCIGGLVGGFVVSQLSKQAIRLAMICSFALIIVLLIAHQLRLLPVGG
                     DLTELHSWKLVIGFFAMVVCGSLTSVGVGLFVMVQGVLFLMNVSPVVAFPIMTTAGAM
                     QQPLTTLVFLQKNKIPLKKTLILSFSGCLGVMITIPIFTKLTITWLHFLLLFILIYNF
                     LAIGHTYLRSRPVKQYVQSSVKLAAAD"
     gene            complement(15575..17251)
                     /locus_tag="VAH76_000455"
     CDS             complement(15575..17251)
                     /locus_tag="VAH76_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M4 family protein"
                     /protein_id="HEO1385108.1"
                     /translation="MSKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKN
                     AIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSN
                     DNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNN
                     QLKLVYLVSFKSVQQDGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYW
                     YGKDGLPALDVTQNGSQCVMENSKVKLVNLHSQWDWENTINTPFEYVCNNNIEEKING
                     GFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAM
                     SFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLL
                     EKNPQLYNKAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNN
                     GSYCAISYDEVVAYANTHIALPQERQSFIVHTASGVFNKAFYLMSKDMGIKNAYHIMV
                     VANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI"
     gene            complement(17477..18883)
                     /locus_tag="VAH76_000456"
     CDS             complement(17477..18883)
                     /locus_tag="VAH76_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1385109.1"
                     /translation="MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNID
                     SKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLD
                     LMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
                     QIAASYFTYLAEIERKEFLQTLANDLTQLAKVANKVYQGGLSSEIEQEELYSQVNLIQ
                     GELEVTERNIVISRNALRYLLNQNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDM
                     QVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPI
                     IDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHL
                     AKAYNLNDRLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYR
                     ANLESEIKSSGRATRTRL"
     gene            complement(18867..19772)
                     /locus_tag="VAH76_000457"
     CDS             complement(18867..19772)
                     /locus_tag="VAH76_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO1385110.1"
                     /translation="MRRWLGVQAFSYVELRSIQISTVFAFTIFVQEWLRYPRAGWTGF
                     AVMMIYAGFDNGTTIFRTYHRFLGVLLGLFTGYLLWFVGHLDYRTLILIMPLMVFFAF
                     FLVGRAYSVPTVFTVNVSVLGSGYFSTEDTLPVTYFIIDYTLCTLIAFTIILGFEYFW
                     FRHYGLMQRFIKSTQAEVIDDLYRLVRLLNQGKIKKTEWFQACIKLTDSLFEVNKLIT
                     NSQFLISSEHAVGDEFNQFVELANRIFIGLKALYMAYYTKHYHTFDYYQLFQQVQKDL
                     VHLKDFVAGEQTIDLSEGVKHATSG"
     gene            complement(19757..20689)
                     /locus_tag="VAH76_000458"
     CDS             complement(19757..20689)
                     /locus_tag="VAH76_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO1385111.1"
                     /translation="MFISQLKDKLASYDRYKEHRINGLKAVFVLELMILFYFFSSIDN
                     PYFYYFYAPITCFTVEAAGTTLKEKYMLLLYTLLGSILSIFLFGIISVYNVLFIFFVF
                     FYTLALYFLVLHHFKKMLVIVPLILSLASYSLIYGVEADSNFYIAINNALYTLVAMAV
                     IFAGLFIFPKKYYFDIWERAFLEVITGLESLSAKICKGEVKTIAIFSGIIVMERYSKM
                     LPRNIKCYSILKITILSFELILTMSYLLSFQKQLRKEYIIVLHHYLERLCQSCKARQP
                     LILSDQELPAFKETHELEILYQLILSWNYLCADG"
     gene            complement(20664..21737)
                     /locus_tag="VAH76_000459"
     CDS             complement(20664..21737)
                     /locus_tag="VAH76_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEO1385112.1"
                     /translation="MKEKFATWYKIATKHIRFFFSYSIPMFFKKAKNYINLPIIVVGL
                     ALIIGLFHLFSYLIPFTDNAFVVTNVTPVAADVSGFITEIYVKNGQKVKKNDPIFSVY
                     RVPYELAYQQAMADYKEGLKKIDVFQKQIERTVALIKATDSELEKAQFALGLKKNKNV
                     AEAVSVLELKNLGYDVSTLSNKRNALQQEVAVLQAKIEQQRHTVASLKAKMDNAKVNL
                     DLTIVRAPGDGVIDNMYVSPNTPIEIHKPVFSFIDTANYYIQANFNETDLRNVRQGDK
                     AYIILRMYYFDKIFHGVVVNSLWAAERQSTAPKSQIQVVTNQNEWLLLPQRFPLQIKI
                     LDPDPDYPLNPGASAYVYISTKR"
     gene            complement(21839..23050)
                     /locus_tag="VAH76_000460"
     CDS             complement(21839..23050)
                     /locus_tag="VAH76_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1385113.1"
                     /translation="MSLEVEPKPHGNNPYANRFDNLPSKAQGRFPRVAYVLQGGGSLG
                     AYQFGVVKGLLEAGYEPDWIAATSIGAIQAAIIVGNPPEKRIEKLERFWDEIAPGNFF
                     DFLGESDITLDFYNKLSAACALLFGQPEFFFPKWCTGTIPLWGDPTTLSYYDTTPLRK
                     TLLDLIDFELLNSCPIRLSLGAVQISNGHLIYFNNINYRIEVEHIMASAALPPGFPAV
                     NIDGELYWDGGVHSNTPLEVILEAIPAENTLCFLIDCFGGRSFIPKSMSEIEERRKDI
                     SYGTHAQRTIYNYLQRQKMRNSMMELSRILTDEQKKESAHLLDIGSPHHCTLVHLAYS
                     SRIVKAASKDYNFGRVVIKKRMESGYSDAKAVLAEESQWGYLPEDGKSRLYESPNNLS
                     RLFRKRKKEVF"
     gene            complement(23487..24326)
                     /locus_tag="VAH76_000461"
     CDS             complement(23487..24326)
                     /locus_tag="VAH76_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEO1385114.1"
                     /translation="MKAIEVERLIQGTLIREGGGVKLRRYIGADKTNHFEPILLFDYF
                     DSSDPLDYLAGFPPHPHRGFETITYLMEGSITHEDNKGNKGVINAGGVQWMTAGKGII
                     HSEMPSSSTGRLHGLQLWLNLPAAEKWREPRYQEKSSDQLPIEVMESGATIKVIAGRI
                     DQGLSSPIVEIATRPLFLDINLPANATIQQTIPDDYQAVLFVIEGAVNTGGQQITAGT
                     LASLTNGERIQIEGKDKENRNHCLLIAAAKLHEPIVRYGPFVMNTQEEITQAIHDFRT
                     GHF"
     gene            24472..26049
                     /gene="cegC1"
                     /locus_tag="VAH76_000462"
     CDS             24472..26049
                     /gene="cegC1"
                     /locus_tag="VAH76_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC1"
                     /protein_id="HEO1385115.1"
                     /translation="MNTTEHTELGNGLLMDKIEGNPYLRIDEFGVLHLRLQRFGENNL
                     PEPMELEMSAGEIIAMAGDYFTQANWTMDLDLPKCELFNSPAELGKHLIRKPIDPKEE
                     SALITAYNNLAAPDVTRKEIDRIYSINNANYVPFSPTLNFYAQQLMYYFRVKDYGEML
                     VRNQTHFTPWSIRVYILGHAIALRYAHLSYELKQLATDRNYQSDNPDLSALKISLQNK
                     NETLSSNTLLDLANRYHAQAYSIELFTFHYYSDHFATGHMSMIGDLRVVLKERFGVWG
                     NILANNLHDEVNRVGVYTVRPYDPTPNATEAPSRARGDGKFDTCLNQFNRLACLNGMT
                     ASLKDINQVLSGSVIPEQKDFGGLVHMPDVDFNSRQHQPLLVLSKGKVYYRNNLSQIH
                     IISPSEYEALRESPEKHGYKELTSKWTAFKLVTKLRLFPYLYDSSVMPVSDEKLAEII
                     ADEKRRNPQRAPIPTPSCLPESEPTVFDWRTKASWRNNKDSLDILDGLKKHSILGAKQ
                     THSAIQEEEEVRLNLGV"
     gene            26218..26688
                     /locus_tag="VAH76_000463"
     CDS             26218..26688
                     /locus_tag="VAH76_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEO1385116.1"
                     /translation="MKRLIPTFIYLLALAITPFSTHAEALLKDTFGHNIPFSSLKGKW
                     VFINYWASWCETCVDEIPEFNRFYKNHKNEAVALYAVNYDALPLPEQKLLIKKFNINY
                     PNLLKDPAQDLQLGDITGVPVTFVFNPKGQLIKKLYGGQTAKTLEKVIAENRTS"
     gene            complement(26793..28037)
                     /gene="hflX"
                     /locus_tag="VAH76_000464"
     CDS             complement(26793..28037)
                     /gene="hflX"
                     /locus_tag="VAH76_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO1385117.1"
                     /translation="MFERPQGGERAVLVQLALPGVDADKALAEFEELALSAKAEVLDC
                     VLGARATPDAKYYVGKGKAEEIAHLVNMLDADLVLVNHELSPSQERNLERLFGCRVVD
                     RSGLILDIFAQRARTFEGKLQVELAQLQHLSTRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRERIRYINKRLEKVRCSRDQNRQARRKASMPTVSLVGYTNAGKSTLFNVLT
                     GEHTYVADQLFATLDPTMRKLELPGSSAAILADTVGFIRDLPHHLVEAFRATLEETQQ
                     ADLLLHVIDISDPNWRETVFEVQKVLDELKVSNIPVIQVFNKIDLQEGWQPKIDYTED
                     ACKVWLSATTGAGLDLLKEAIATQLHGMILIEDIVIKPDQAKLRAQLYQLGAVLKESV
                     DENGDWLMKIRITADQKRRLLS"
     gene            complement(28065..28322)
                     /gene="hfq"
                     /locus_tag="VAH76_000465"
     CDS             complement(28065..28322)
                     /gene="hfq"
                     /locus_tag="VAH76_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEO1385118.1"
                     /translation="MSKNHLLQDPFLNELRKEKVPVSVFLVNGIKLHGIIDSFDQYVV
                     MLKNSITQMVYKHAISTVVPSRMVKIPAEESSGEEEGTVAD"
     gene            28510..29769
                     /locus_tag="VAH76_000466"
     CDS             28510..29769
                     /locus_tag="VAH76_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385119.1"
                     /translation="MTFTCDELKGLEHPYKVLGNGDALAENREELNKLTNDEKLVLAS
                     RLVLECPVNELKDFAHAIEAARMPQDDSDTFHSFLFQAYQVKKRIISLLDPRNKNPHS
                     MILEKEFDGELFNNFNKLAIDVLTNNEVAIALRLAETTPAQDRSRVSQNINNIFPQSL
                     FAAKVGHAFAVRRDIERLLLGDRPDQFFSSREFKIDSCIEFASLFNVINDKESSIAGK
                     LALRTPAENRSDVVMKIKGFCAEDSELAIKVQSAFALRRDIERNLLGDNPEQFFNSRD
                     FSVDLCLEFAILFPELLKGHEQAIGEKLAKLDAKVRSDISRKLEMINGAAHEQSSPFR
                     IIASAMVPKEEPCLTKPTPVVLPSTEEIIPKQESPTSLRNRNVLFKEQTEEHKKKNSQ
                     MEPIASDSEEEEEKQNCWTSFCGLFGK"
     gene            30310..31155
                     /locus_tag="VAH76_000467"
     CDS             30310..31155
                     /locus_tag="VAH76_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEO1385120.1"
                     /translation="MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQ
                     ELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVA
                     FNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPEL
                     SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCED
                     GLSFYGSSFISTPMGEILVQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHE
                     LVKSR"
     gene            31175..33067
                     /gene="speA"
                     /locus_tag="VAH76_000468"
     CDS             31175..33067
                     /gene="speA"
                     /locus_tag="VAH76_000468"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEO1385121.1"
                     /translation="MNNNTLSDDNLYNIENWGEGYFRINSKGNIEISKKPGKPGVELQ
                     TIVDAADRAGLHLPLLIRCSDILHDRVHRIYQAFTQAREDTYYAGNYQLVYPIKVNQE
                     QSVVRELLKSPDNQIGLEAGSKPELMAVIGMLGDHQSTIVCNGYKDTSYIRTALIAQQ
                     MGHKVFIVIEKKSELDIILKESARLQIKPTLGVRIRLVSKSAGKWENTGGVKSKFGLN
                     AKQVLELILQLKANDMLDCLQLMHCHLGSQIANIHDIRHCMQEVARYYIELRQLNAPI
                     GTIDVGGGLSVDYEGTRSNKGCSMNYSLVEYATHIMLALKHLCQEAEIPEPDLISESG
                     RALTAHHAVLISNITDVEIINKAPVLPEISEDDSHVIRDIYDTYQAITDSSPTEIYNY
                     AAHSLNEAHSLFKHGVISLQEKAKVEAFYTNICMEIKDKLDEENPSEKALLMRINECM
                     AAKIFCNISFFQSIPDAWAIGQIFPVAPISQLNRTLSMHSILQDLTCDSDGTIKQYPG
                     SSCVTTTLMLPSYDINNPYNIGFFLVGAYQEILGNLHNLFGDTNSLDVKLSDDGQFEL
                     DDLVSGDTVTNVLNLAHFDTKKLIQSYEKQLIKSELPKEKALSYLNELRSIFSQLTYL
                     DGNIAQ"
     gene            33070..34110
                     /locus_tag="VAH76_000469"
     CDS             33070..34110
                     /locus_tag="VAH76_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEO1385122.1"
                     /translation="MNTAPKQYGFYMPAEWYPHERCWMAWPCHHDTWSKIGLDKAKMA
                     YARVAKAIAQFEPVTLLVNPGDEDSAENLCKGHNIEIISLPINDSWTRDTGATFLINN
                     ERQLAGVDWIHNAWGGNYADCSLDNLLASHLIKYTEAQYFHAPLVMEGGSFHVDGEGT
                     ILTSKECLLNSNRNPHLSQQEIEQYLINFLGAERIIWLNMGLIGDETDGHVDEIATFI
                     APGKVLCLITKDKEDPNYHRLQENFEILKSSKDARGRTFEVYTVEQPPATYLNGERLT
                     LSYINFYMANQGIVMPAFGYESFDRLAYQLFVQIFPGYQITQIDALDVFSGGGGIHCI
                     TQQQPKSYKLVG"
     gene            complement(34474..36891)
                     /gene="gyrB"
                     /locus_tag="VAH76_000470"
     CDS             complement(34474..36891)
                     /gene="gyrB"
                     /locus_tag="VAH76_000470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO1385123.1"
                     /translation="MSVDASYDSNNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGYCKEIFVTIHSDESITVKDDGRGIPVDIHKEEGKSAAEVIMTVLHA
                     GGKFDDNSYKVSGGLHGVGVSVVNALSEELHLLVRRHGKVYEQHYRHGVPESPIAETG
                     ESNSTGTQIWFKPSAETFSNIEFHYDILAKRLRELSYLNSGVCIHLFDERTQKKDTFH
                     YEGGIKAFVEHLNKNKTPVHPVVFSMTSEKDGIVIELAMQWNDGYQETIFCFTNNIPQ
                     RDGGTHMAGFRAALTRTLNNYIENEGYAKKAKVSPTGDDAREGLTAVLSVKVPDPKFS
                     SQTKDKLVSSEVKPAVESCVAEKFNDFLLENPTAAKSIVAKIIDAARAREAARRAREM
                     TRRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQARDRKFQAILPLKGK
                     ILNVEKARFDKMLSSQEVATLITALGCGIGPEEYDPDKVRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQTRELLERGYIYIAQPPLYKVKRGKQEQYVKDDEALFDYLTQCALDGAT
                     FYPAKEAPPISAKSLEELVQHFRRVEKIIKRYKRRYPSDILKRVAYMPMLHQEDFNNL
                     SKIESWCRQLHLSLQQVDSKTEQYKVDIQKLTEGNQYTPKITVYQHGLENDIPMQVEF
                     FLSKDYKEMVDLGHKLSNLIEEGAYIQRGDKSLPVENFEQVLNWLMDEAKKGQTIQRY
                     KGLGEMNPEQLWETTMDPEVRRMLQVSIEDAVAADEIFTTLMGDNVEPRRDFIETNAL
                     DAENIDI"
     gene            complement(37188..38249)
                     /gene="recF"
                     /locus_tag="VAH76_000471"
     CDS             complement(37188..38249)
                     /gene="recF"
                     /locus_tag="VAH76_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO1385124.1"
                     /translation="MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALY
                     MLSCAHSFRSREVAPIISYGQNQLNVFAHTYDESTISVQKSITDGTQIKLNNQFCCTT
                     SQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
                     LLKSRATYEHFIPWDQQLSQLANQLDKARNEYFLQWQPKFYQVLSDLTNISCTIEYYK
                     GWDRKNAGQNMEELLQKSFDSDKNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKII
                     LIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKGQFVITNLINNNNYD
                     ILPIDSGLFEVNCGAIKVL"
     gene            complement(38246..39349)
                     /locus_tag="VAH76_000472"
     CDS             complement(38246..39349)
                     /locus_tag="VAH76_000472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO1385125.1"
                     /translation="MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLY
                     ITATDLEIEMSAQVACDSNNTSGSITVPAKKFIDIIRSLDENPSPSIVFDKALVTIKQ
                     NRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
                     LEFDSNIISAVATDGHRMAISRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLA
                     AGKSHIKLITNQFVFLSKLIEARFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAH
                     EKSKAVLLHLQPGQLTLIANNNEQEEAIETLSAETQGDELKIGLNATYLLDVLAHFSD
                     GQIRLSMSNTESSILIESLVNDNYQYIIMPMKI"
     gene            complement(39363..40721)
                     /gene="dnaA"
                     /locus_tag="VAH76_000473"
     CDS             complement(39363..40721)
                     /gene="dnaA"
                     /locus_tag="VAH76_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO1385126.1"
                     /translation="MSTTAWQKCLGLLQDEFSAQQFNTWLRPLQAYMDEQRLILLAPN
                     RFVVDWVRKHFFSRIEELIKQFSGDDIKAISIEVGSKPVEAVDTPAETIVTSSSTAPL
                     KSAPKKAVDYKSSHLNKKFVFDSFVEGNSNQLARAASMQVAERPGDAYNPLFIYGGVG
                     LGKTHLMHAIGNSILKNNPEAKVLYLHSERFVADMVKALQTNSINEFKRFYRSLNALL
                     IDDIQFFAGKDRSQEEFFHTFNALLEGQQQIILTSDRYPKEIEGMEERLKSRFGWGLT
                     VAVEPPELETRVAILISKAEQSNIELPYEVAFFIAKRIRSNVRELEGALRRVIANAHF
                     TGKPITIEFVHEALRDLLALQDKLVTIENIQKTVAEYYKVKVADLLSKRRSRSIARPR
                     QMAMALSKELTNHSLPEIGDHFGGKDHTTVIHACRKVKELIQDDSDFAEDYKNLMRIL
                     SS"
BASE COUNT        12762 a         8310 c         7384 g        12353 t
ORIGIN      
        1 gattcgtact taatcaaagt ggcgaattta ttttttatca tttaacaaaa gactttaaat
       61 atgggatgga ctgatcgatt ttggaatgcg tatcgcgtgg ttactaatac cgtaatcgaa
      121 ggcgcagcaa actctttaaa tgcagctgga tcactagctt gcctgctcgg aggcgctggg
      181 gtctctctct cttactccat gtctgaaaca ataaaagcag ggtacttcgg atcagttgga
      241 gtagcaggca cagttaattt ggatgtgact gcagtagaat ttaattatgg atttaatcaa
      301 accattcctt ttcgcagaga tttgctggaa aaatccggcg gtggcagcta tgcattgaat
      361 gattttgcca atccaggtac tatttttatg attagttctc tggctattct ttccggaacc
      421 actttaagaa cttttggggc taatcttaaa aaatggcaac aaaacagaga agacaaatac
      481 cactttattg ccaagtacag cactgacctg gaaccccctt cttacaaaga atatctgtat
      541 gtcaacgcag aatccttctt caattctttg gcaatagcct cattcagcag tcttttggca
      601 aaaaatgttc tggatttttc gattaaggat ttatcgaaat atcatttcac ctatcctttt
      661 aatggaacaa atataactgg ctcagtgaaa acgcctgatt atactgggcc agttacatca
      721 gaatttttcc cccttgactt ttttgtgtct caaaatacaa ccatccacac agattatttc
      781 aacctgatag tcaatgaaaa agtgaaggcc aatgcaatga ccaatgtcac ttatggtgga
      841 ggagtacatt tagagcccag ccaccaatca agcgcccccc ttctggctgc ttctgcaact
      901 attggtatcg gtacctattt ggcaggcact ttttttggca agaaaatcct gcgagaacgt
      961 gatgaaagac ttcaacgttc aagatcatcg agctattcca tagtttcagg cactttttgg
     1021 gataataagc atgacataga ggcaaaaggc gatacaaaat tcgagcatac ccattcatta
     1081 cagtaaagaa tagcctaagt ctcctccatg taatatattg caatgaaaaa ataaaacgga
     1141 gtttttcatg gtatttaaat caaggttagt tggcgttatt ttattgtgct tttcaagtat
     1201 taccggatgg tgtctttctc agtcccctga agaggataga atcattgttt caaactgcct
     1261 gtatgagcaa ttaaaagaca aagccaggtt aattgcgcaa tcggatcatt ttagcttcat
     1321 tgccattcca attgatgaaa atattaccaa tactctacaa aaaatgagac cagtttgtgg
     1381 aaattatctt aatgctgagc catatatcca acaaacatcc aatcgttttc tcgattcgca
     1441 aaaattattg gataaactga ccagctacca ctccatctca accgcaaacc catacccgat
     1501 taatcatgaa gcacaagtcc atggtttgct cgaacaaatt gacccggcca aaatatggca
     1561 aaccaatcag catttaacca gttacatcaa ccgctctgcc aaatccagaa cgggagtgga
     1621 ggctgcacaa tggtttaaac agcaatttga taccttagcc caagattatg gaagaaaaga
     1681 tgtagagagc tacttcgtca aaactggaaa taaatttata caaccatcag tcgtcactgt
     1741 aattggaaaa gataaaccgg gagaggccat tgttataggg gcacatattg acacactgga
     1801 cggtaatatg cctggtgccg atgatgactc tagtggaata tccgttgaac ttgaaatggc
     1861 aagagtgctc ttttcatcga actttgagct caatagacca gtttatttta tagcttatgc
     1921 tgctgaagaa agaggactta tcggctctgg atatgtggta caagattttt tacagaaaaa
     1981 aattccggtg aaagcagtaa tgcagttaga ccaggcagga tacagagcta acgccaaaga
     2041 ccaaacgatt tggctgttaa aggattatgt agataagggt ttaactgaat ttacagcaga
     2101 attattaact cgctatgtta aaattcctgt tggctatact aagtgcggtt atgcatgcag
     2161 tgatcatgtg aattggacca atgaaggctt taaaaccacc tatccgtctg caactacttt
     2221 agacgatgat aatccttatg ttcatacctc caatgatact ttggatattc tgaatctgga
     2281 acatatggtt aatttcacta aactaggcct ggcctttata gtggagcttg gtttaaatta
     2341 gcgatgaaag caatagagcc cgtgccaatt taatggtgct ctgcaattgg agtttattaa
     2401 tgaaaacaga agccttactt agctggctaa acgtagaaag caatgaatcc tatgattcta
     2461 tcagaataaa gtatcaacta agacttgaag ccttggagaa agaggaacgc attaaaaaaa
     2521 tggggggtat caatcctttt gcgcctaaaa taccccttaa caacaccagg ataatcacct
     2581 ccaaagaaat acaagaggca tgggctgcta ttgattctct cagcaagtac caacaattca
     2641 aaggactagt ggctcaagaa gaaaatttat ccactatccc taacaaaaaa atggatagca
     2701 agagagagga aacaaaatta atccattttg taagagaacg tgcacaaggc cctcagggaa
     2761 gacgcttgcc cgcccattta caagctaaaa ttaaaagagc ccctttggac aaaccacaca
     2821 gtaagctaat tactgaaggg gtcgaacaac aaattaacgc aattttagac aaaaccacct
     2881 tgtcagcgag tgacttgcaa tctgctgttt acaccgcagt ccagaattat acgaattatc
     2941 acatgaaatc cgaaaataaa aataatccca aatacaacag aggacaaggc gatggttttt
     3001 tcagtttttt tcgccatgga agctttggaa tcactacagc gaataaccta tacacttcca
     3061 tcagcgcaaa aaatgctact ttagatgaaa ccatagaaaa attaaaagat tttttaggcg
     3121 attcctccag agcatatcat catcattctt tcacatctta cctggctgat gcacttgctg
     3181 agaagggact attaacatcc catcctgcca gccggtataa acaagaagac gtagtgaacc
     3241 agcttgacca atggatagca caaaactcaa ctgttaatct tttaaaatga taatggcaag
     3301 gatagtctgt tttttactcc tgaagtaaaa acaaataaat tagaatccgc caccgacatc
     3361 tgtgccaaat ctttaaatta ggtacatatt tacttattca ttaaaaaaaa caaaaattta
     3421 tccccactca aaggcttgga aaaataccat ccttgtacca tatcgcagtt cattgagcgc
     3481 attaactcca gctgttctgc tgtttctatt ccttcagcaa taaccgataa tttcagattg
     3541 cgcgccatag ttataatact attgatcaaa cttaactgat tatgatttgt atttacaccc
     3601 tggataaaaa cccgatcaat ttttaaattt ttgaatggat aggatttaat atactgcaag
     3661 aaagaataac ccaccccaaa atcatccaaa gaaaaatgaa tgcctatgtc acttaatttg
     3721 tttaattgag ataaaatgaa ttttgaatca tcaataaatg ccgattctgt aacttcaaaa
     3781 attagagaat ccgcagataa ttttgttctt ttcaaaatga gttgcacctt atgaataaaa
     3841 tccttttgct ttaattgtaa ggtagagata ttcaccgcta cttttatctt gctgccggta
     3901 ataaattccc actctttcgc ttgtgtgcac accttatcca aaatccaata acccaatgga
     3961 ataatcatac cggtttcctc ggccaaggaa ataatctgat cgggattgat ttgacctaag
     4021 gttgtattct gccaacgtaa taaggcctcc gcagcgacga ctttattttc tttcagatca
     4081 atgatcggtt ggtagcaaat atatagctca ttacgggtaa acgcctgatg taattccgtc
     4141 ttaatacgga tatggttatt tgccccatca atcatggttt tttgaaatac cgtccaatta
     4201 tttttgcccc gtatcttgct ctgatacatg gctgtatctg cattacgcat taaagttttg
     4261 gcgtccttgc catgatcagg atatattgca attcctatac ttgtagacac caatagctct
     4321 tgtgattcaa taacaaaagg ctgagaacaa atttcccggc atttttttgc gatactttca
     4381 gcatctttat cctgcatgat gttttctaaa atcatcataa attcatcacc acctaagcgc
     4441 gcaatagtat cggtttgccg tatcatagat agaaagcgct ccgataacgc ttttaataat
     4501 aaatctccaa tcgcatgccc taaggaatca ttaatctgtt tgaattcatc taaatctaaa
     4561 aacagcaccg ccaatttttg attattgatg tgggattttg aaatagctcg ttccagttta
     4621 ttaactgcat agaggcgatt gggaaggttg gttaaattat cgaatgttgc ctggtgtttt
     4681 aacatttcct cgtagctttt cttctgagtc acatcctcca taacggccag ataatgagtt
     4741 atctcttcat tctcattacg aattggagaa ataattgctg atacccaaaa atgctctccg
     4801 gatttgtttt tattcagcaa ctcaccatgc cactcttccc cattggctaa gtgttcccac
     4861 atggttttat aaatttcatg gggagtttgg cctgatcgta aaattcttgt atttccacca
     4921 accaattctt ctttgcgata acctgtttgc tctatcactt ttgcattgac atactcaatg
     4981 gttcccgttt tatcagttat tatgactaaa acagggcttt gctctaacgc tcgtgttaat
     5041 tttcttatag ttgcttcaga gcgtttttga agtgtaatat cccgtacgat agccaaaacc
     5101 aatttctccc cagtcgcatt aaaaactgcc aaacggacat caacaggaaa aattgtgcca
     5161 tcttttctgc ggtgcatgcc ttctgtatta atggggccct tttgaacatt ttcccacaac
     5221 tgatttaaac tttcctgaga aaatcccact tcaatatccc aaacatagga attaattaat
     5281 tcttcccggc tataattcaa agacaaacaa gccgcttcat taacttctaa aaatgctccc
     5341 tgcagattat ggataaaaat accatccgct acagcattca tcagatttcg atatcgttct
     5401 tcactgattt ttagttgatt gaatcggtgt acttgttctg taatgtcttg tttaattgct
     5461 atatgttggg taattctccc caattcatca tccatcacag gaacgatgat ggtttgagcg
     5521 acccaatagc tgccatcctt ttttcttgag cgtatttgcc ccttccatgt attccctgat
     5581 ttaagggttt ggtttatttc ttcaaacaac tgagcgtctt cgaccggtga gcgcaataag
     5641 gacgccactg ttttattgat tgcttcacga gcattataca aagtgatgtt ctcaaaagcg
     5701 ggattaacat attgaatgac agcctcttca ttcgttattt cgaccgagtc gccagctttt
     5761 tccaaaatag tggtcagcag cttttccctt tttttattca tcaatttggc taaagacagg
     5821 tttaatatac ccagaatatc tttaaaaaaa ggcattagta tttcaaagtt acagtcaata
     5881 gaatcaaacc aaaaactaat acaaaataaa ttcccttgac atttatcaag gcaaatatcg
     5941 atacgctgct ttaatttttc ttcagaatgc ttcttgagct ttgtcttata tatttttaaa
     6001 aaatgccaat ccgagactac agctaaaaat ttctccaatt cctttggcgc atcaaatttt
     6061 aatccattag atgccagaat attaatatga ctgccatcat gatgccaaac tgcggtgatt
     6121 gtgtctttta ataagggatg cttgccaagt aattccaacc cacttaagct taaagtatgt
     6181 agggtatttt catcataact caattcaatc aattggctta ttaagcttcg ttctaacagg
     6241 ttaagatggc tgaaattatc tcctgaagca atatgattta atggatgtgg ctttttactc
     6301 ttcctcattt tatctatcat gtgccaattt agacctacac ctatcatttc tgtataataa
     6361 agcttaggat cttattgtgt aataatcaaa acagatgtat aaaatttact ccatcaacaa
     6421 ctccatctta actcgcagcc accgatatta aattacctta taaaatttaa ttgtctaatg
     6481 acttcaaact acacactaat caagttaaca tagagacctt acactttatt tttaaagtat
     6541 ccatgcgaac atcaagcttc ctcgatagat taataggtga agtcgatagt gcgcttcgaa
     6601 ccctcgtttt gccacaaaaa cgtattacaa cgcgccaatc tcccgctgaa aacctggctg
     6661 atactgtgct ttctgctcag gaaaaaaaac atatttcagg attaatgaga gtaaatcatg
     6721 ccggagaagt atgtgctcaa gcgctttatc aaggacaagc tttaacagca cgactcactc
     6781 acatcaaaga gcaaatggct agtgccgcgg ctgaagaagt tgatcatttg gcctggtgcg
     6841 aagaacgttt gtatgaatta ggcagcaaac ccagcctttt aaatcccatc tggtattgtg
     6901 gttcggttct attaggcgcc ctggctggat tggctggcga taaaatcagc ttgggattcg
     6961 ttgctgaaac agagcgccag gtaaccgctc atttacaaag acatctgcat tatctcccgg
     7021 aaaaggataa aaaaactata gccatattaa aaagaatgca agaagacgag gaacatcatg
     7081 cgcatacagc catggaagct ggagcagttg aacttcccta tattattaag caattgatga
     7141 atgctgtatc aaaattaatg actcagagca gttattatat ttaacaggat atgattacat
     7201 atggactaca atacgatatt cattctattt gtcatattta ttgcgttcgt atttgatttt
     7261 atcaacggat ttcatgatgc cgccaattcg attgcaacca tagtcactac aggtgttttg
     7321 acgccaagac aagctgtact atgggctgct ttttttaact tcattgcttt tctgattttc
     7381 aatttaaccg tagctcaaac aataggctac ggactaatcg atacggatct ggtggatact
     7441 tattttattt tagcagccct gatgggagcg atattctgga atttggtgac ttggtattat
     7501 ggactgccat caagctcctc tcatgcctta attggcggat tagctggtgc ggcaatagcc
     7561 aagggaggcc tgtcttcact taagattgca ggttttgcca aagtagcggc agggatattt
     7621 atatctcccg ttctgggatt attgattggc ttattattta ctcatatttt ctctttgttt
     7681 ctcagacata agaacgaaga ggaactgaat acattattta aaagttttca actgatatct
     7741 tcagcattat tgagtatcac gcatggaggc aatgacgcac agaaaacgat gggtatcatt
     7801 gcggtattac ttttttccgc atcttggctt gatggtgtat tttatgtgcc attttgggtc
     7861 gtcatttcat gtcatggggt aattagtttg gggactttgg ctggcggctg gaggattgtg
     7921 cataccatgg gaactaaaat taccaaactt aacaccctga agggttgtgc tgctgaaaca
     7981 ggagcagcta ttaccatttt cgctgcaaca gaatttggag tacctgtctc aaccacccat
     8041 acggtcactg gctccattgc gggagttggg ctaatcagtg gcataggtgg cgcccactgg
     8101 aaagttttac gcaggatatt ctactcttgg ttgcttacca ttccagcagc agcattagtt
     8161 gctgccgcta ttatgaccat ctcaccaact gcctagatta tggcgtcact cagcagcaca
     8221 atctccctca acacttaagg cactatttgt cctgctaaat gcaaaataga ccgctatacc
     8281 aactagcatc caaattataa aacgcaatat cgttacccat ggcaaattca aaattaaata
     8341 aaaacagctt attataccta atataggaac ataaggcata aaaggtgttt tgaatggcct
     8401 ttccatatca ggatgagttc gccttaaata taaaacacct ccacaaacaa caataaaggc
     8461 aaacaaggtt ccaatattaa ccagctcggc caaggtacca attggagtta ctgaagcgag
     8521 agatgccatt aatatgccac atagcaatat aattcgaatt ggtgttttag tgtgttcgtt
     8581 cgttttggat aagaaccttg gcaataagcc atcccttgac attgctaaaa atactctggt
     8641 taatccatag aacagaacca acataaccgt ggttaaacca gcaattgcgc ctacaccgac
     8701 cagtccggct gctgttttat aacccaagac aagcaggaca tgactgattg gtgatgaaac
     8761 gttaagagta gtgtaatgag caatcccggt taataaacct gaaaccagaa tatagatcac
     8821 tgtacaaata gccagagaag cgattatccc tatgggcaaa tcccgttgag gattaattgc
     8881 ttcttcagca gcagtagaaa cagcatcaaa ccccacgtag gcaaaaaaga tgattgaagc
     8941 acctttcata acaccagccc aaccataggg catgaaagaa gaccaatttt caaccttcac
     9001 ttcaccaaat gcgacagcta tgaaaataaa aataaccaaa agcttaacaa gcaccatgat
     9061 attattaaat cgagaactgg atttcacacc aacaattaat aatgtagtta aaacagctat
     9121 gatcaaaatg gctaaaatat tgagatcccc accgtcagca ggaccatgta ataaattagc
     9181 cggaataaat attttcagag ccattaaaaa atcattggca taaccactcc agccgataga
     9241 aacagccgat acagcaatag aatattccaa taacaaatcc catccgacga tccacgcgat
     9301 taattcacca aatcctgcgt aagcatatcc ataagcactg ccacatcctc ctatgctggc
     9361 agccaactca gcataggata aggcagcaaa agcgcaggca aatccggcaa caacatagga
     9421 gaatatcacg gctggtccgc ttatagtggc agctactatt cctgtaagaa cgaaaatacc
     9481 agcacctata atcgcgccca ctcccaaaaa ggtaaggtca aaaacactaa ggcatttagc
     9541 taaatgtgat tcattgtcta gcgacccttt tatatctttt ttacgaaaca agtccatgat
     9601 agtatgctct caggtttatt ttttattgtt tatttccaaa atttaagtta tcaataaaca
     9661 taaactccgg ttatcttcca tctaacaaaa ccttacacaa ttaaactcta ccattaaatt
     9721 atagctggga gaaaccctgc aaaggttaaa aattgaatga aaagattaat ttcctgctta
     9781 accattattt gcgctttaaa tgcatccgct gcagcggaaa cgacatccaa tccttgttct
     9841 cgctggataa gtttcctcaa gcctgtctgc caacgcatcc accaaacatg ggcagaaggc
     9901 catgatgata tgtatttttc aggctatgcc tggcataacc gctatgttta cagtaatgaa
     9961 aaaattaagt cctacaatga aaccgcatgg ggtggtggat taggtaaaag tctttttgat
    10021 gaaaagggaa actggcatgg gctttacgcc attgccttcc tggattccca tcgccattta
    10081 gagcccgccg ttggttacgc ctatctcaaa acagcttcag tcaataaaga tttaaaagca
    10141 ggccttggat actcggtatt agttacctct cgggttgatt atgataatgt ccccatcccc
    10201 ggagcattgc cgtgggctgc tcttttttat aaaagaataa cgatcgcagc aacctatatc
    10261 cctgggtcca gtcgtgaagg acatgagaac gggaatgtat tgtatatgct cggaaaaata
    10321 tccttataaa gatggattaa cttttgcaag ctgcaaaagg tcatgtgaaa caaaccctga
    10381 ttagatccct ttaaaacggt tgcaactgaa aattcaactt gttagtcttt tgaagatttt
    10441 taactgctaa gtaacaagga gaagtaacat gatgttgaaa acccagttga ctgcttttat
    10501 cggtgctgta atcttggctg gctcttcttt agcaaatcca ataaaacctg aggtatgccc
    10561 cagtgtaccc tctattcaat cggaaggaat gtccatgtct tctgaaattt tggagggcat
    10621 gtacatcacc tataatttaa gtcattacaa taccagttca agctgggtgt ttattgtagg
    10681 gccaatcgca gctgaaaatg atgatatggc attggcagaa agcaataaat tactttcaac
    10741 catgccaggg tctccccatc cggaagatga tggagaaggc aattggatat gtcagtatac
    10801 gaccaaatcc aaagacatta ttgcatttgc catagaagca gatgatatgc tttctccatt
    10861 gaaaatgatg agatatctca gaacaatccg ctgataagtg gataacacct gcacggacac
    10921 ggaagcatga tttgcttccg tgatttaacg aatagttaag aactcctcct ctctttttgg
    10981 tacttcttta ttttatgtgc tatgttttct tcatgaccct aacttcagga tgaaaagaaa
    11041 atgaaatcgt ttattgagca agctcaattc tatgcaacct accaccaaaa cccagcgaca
    11101 cgttacacac atatggcagg agtcccgtta atcatgcttt ctatcatgat ttttctggga
    11161 tttgttaaaa tagttattcc aggtgtttat gcaaccgatc ttgcttgttt ggctacgctt
    11221 gccgctctca tttattactt cctgttaaac tggcaacttg ccttggcatt aactcccatt
    11281 ttgatctttt tgttatggct ggccagttgg tttaactatg atggcccaac gaaattcgga
    11341 ctttgggtat ttattattac ttttgttgta ggctggggat tgcaatttta cggtcatttt
    11401 attgaaggaa aaaaacctgc ctttatggtt aattttaccc aagcccttat tgcgcctttg
    11461 tatttaacag cggaattgtt ctttatggca ggactcatga actccctgag agaacaaatt
    11521 tacgggaatg aagaagtaaa tactgaagaa attagcaaaa ccaaaacaaa atccaaatct
    11581 taaagagagt cgagcttcac gcttatgaaa gggaagctcg gcaagagatc taataataaa
    11641 taactgaatt attcagttat ccgcaaaatg tggcattaca attttaaaac aatggtgaat
    11701 tttgctattt cgtttaaaat cctgatcgat ggtctgcggt gtaatgtctt gtaccgcata
    11761 tttctctttt agcattggct ctaacttaaa ttggcgcaga ttagttgaga aatataaaac
    11821 accattagga tttaacaacc gcattgccat gttaatcaaa gaaacatgat ctctttgaat
    11881 gtctaaaata tcgctcatac gctttgagtt tgaaaagctg gggggatcta aaaaaataac
    11941 atcaaattta tccctggtaa ttttcatcca ttccttacaa tcgtattgca aaaattgatg
    12001 cttggataaa tcaatatcat ttaatctaaa attatcttcc gcccaaagca aatacgtttt
    12061 ggataaatca acattcgtgg ttaatgcacc ggccaaggca gcatggacgc ttgcactggc
    12121 ggtataacaa aaacagttca aaaatcgagt tcccggctct aattgagcaa atttcaagcg
    12181 cataagtcta tgatccagaa atagtcctgt atcaagataa tcatacaaat tcactatcaa
    12241 tttcgccttg ccttctgtca ctatcagtct ttggctggtt ttacctattt tttgatattg
    12301 ttctgagccc ttttgctgct ttctttgttt aactattaat ttttcagggt gaatacctaa
    12361 ggcccttgga acgacttgca acatctcaag gctgcgtttt tctgccttgt gtactggaat
    12421 gctggctggt ggcgcatatt cttgcagtac agcatagtca ttatatatgt ctatggcata
    12481 ggcatattcg ggtaaatccg catcgtaaat tcgataacag gtgatttgat tttttcttgc
    12541 ccatttctgt aaatgatttc tgtttttttc caatcggtta aatagcattt gtgcattatc
    12601 agaaagcggg gcattcgggg tattttttag tttatttgca gcagaaagag tcaaacaata
    12661 gagtttgcac tctaatgggc cattatataa agtataatgc ttatcagctc gtaatcctaa
    12721 tgctttagcc aaaactggac tggaagttaa aatagccgct tgccaacctt gataacaagt
    12781 atgtaatgta gtacccaatt gttgatataa aggaacaagt tgagttacct cccccaatcg
    12841 ctcaccgtaa ggtggattac aaacaactaa tccttttttt gtccctggcc tacaagcatt
    12901 tagcggcaat gtattaaatt caaccaaagg taatacgcct gctctctcag cattggaacg
    12961 cgcaagagta attattttgc catcagcgtc agtaccaact agcttaactg ccagaggttt
    13021 cacttgctgt aatgcctgag ttcgcaattt ttcccataaa gaactctgat gccgagccca
    13081 atattgtaag gattgatctt gtcgtaataa acctggggca atatgagctg ccatcatggc
    13141 tgcttctata actaatgttc cagagccaca aaatggatcg tgtaagccat atccctgtgc
    13201 ggctaactct ggccatttgg cccttaacag catggctgct gcaacgttct cctttaaagg
    13261 agccttgcct gcttttttac gatatcctct ttgatgcagg ctatatccaa ccaaatcaaa
    13321 actcaccgtt aaaatatcat ttttcaaatg agcgtgaatt aaaatttgcg gtttttcttt
    13381 gtcgacagaa ggccttgacc cattcaagcg acgaaagtga tccactattc catctttgac
    13441 tatctgagcg ccaaacatgg tattacgaat ttgctcagag gcaccatgaa attcaatggc
    13501 tatggtttta tcatgggtaa aaacagtttg ccaatgaaaa tcggtacata actgatgtac
    13561 tgcttgctct tttgcggcat ggccactaaa taaaatgagc tgtattctgt tagcaagtct
    13621 tgaccataaa cataaattat aaattacagg caggctagcc tcgccataga caccttgagg
    13681 actcacctga gtcacatgaa gccccaatcc cttgagctcg tcttccagca aatactccaa
    13741 accttttgaa caactaatga atagagagta attcatttta ttaaagacct taaatagcga
    13801 aacagtgctt tagcagcgcc cgtatttttc tctttcatct gatcatctaa tgccttttta
    13861 atcagttgcc ttaattgttg tacgtcctca ggttgataag catcgataaa ctctgtcaaa
    13921 gcgtcttttc catcttgtaa taacctgtcg cgccatagct ctatctcatg aaaagaggcc
    13981 gtaattgaac tctcttcttc aaggattttc tcataggcag caagaatggc ttcaaaatca
    14041 gctgcacgca ttaacttgcc tattaattgc gcctgtcgtc ttttagcccc atggcttttg
    14101 attgacctgg cgtcaataat tgcttgacgt aaattatcgg gaaggggcaa agaatccagt
    14161 ttggatgcgc ttaaatcaat aaatttaatg cctatatctt gtaaataatg agcatctctt
    14221 tttttctgtg atttactgat ttgttcatcc atagggcgat atatttatta aaataacgtt
    14281 aattatacta tataagtgcc tatccagata ggatagactc taatcaattt tgaataaaaa
    14341 caagcaaata ccaacatcaa cctgcatcat cctattagtt agctatagct ttaaaagccc
    14401 tgttcccaaa aaaacaataa agaaaaatta taaactgaga gactcagtcc gctgccgcaa
    14461 gtttaacaga tgattggacg tattgtttga caggtcttga gcgtaaataa gtatgaccaa
    14521 ttgctaaaaa attatagata aggataaata aaagtaaaaa atgcagccag gttattgtta
    14581 attttgtaaa aattggaatg gtgatcataa cacccagaca tcctgaaaag cttaaaatca
    14641 aagtcttttt taacgggatt ttattttttt gtaaaaaaac taatgtggtc agcggctgtt
    14701 gcatggctcc tgcagttgtc atgataggaa aagcgacaac tggtgaaaca ttcattaaaa
    14761 ataaaacccc ttgcaccatg acaaaaagcc ctactccgac agaggtaaga gagccacaca
    14821 ctaccatagc aaaaaaacca ataaccagct tccaggaatg caattctgtc aaatcaccac
    14881 ctactggcaa caggcgcaat tgatgagcga ttaaaagaac aatgattaag gcaaatgaac
    14941 aaatcattgc cagtctaatg gcttgtttgc ttaactggct cactacaaaa ccacctacta
    15001 acccaccaat acaggttccg gtaactaaag taagtagcgt agttaaatca acatctacca
    15061 tgtgtatgaa aaataaagat tcgatagcgc cagggaggac ttgagcgcca ttatttacag
    15121 caggtaattc atcatcacga aaagtgccta ataattttgc caaggctaca ttaacggcga
    15181 aactgccgac ccccactgta tctgcaataa aggcaataac tccgctacac attaatttaa
    15241 catattggga gaaggataga ggcgaggaag gctgctgcat aaattttaaa accattacgg
    15301 ccaggcagaa aaaactcaat attagtatgc aaagcgtgat taaaaaaaat gccattctgt
    15361 tcctaatacg aaagtaatat ataatcccaa tgattttaaa atcaaaactt attagggttt
    15421 ctattccaaa gaaatctaaa agaaagatcc tgcttgatta tatactaatt tctatgcccg
    15481 gcaaacaaca ctgcgagcaa aaatagctcg cgatgaatca caagggaaca acagcttgca
    15541 ggaaacaaaa atttgccagg agcttttgct cccgctaaat aacacacccg gctgtatcaa
    15601 tacctacttg accaaaagcg gacttaacct tattgatatc ggtatttaaa tccctggcag
    15661 cataaatgac tccgcaagct ccatttttaa agtctgtcgt aggagtccaa tattttgtgt
    15721 tagcaacaac catgatgtga taagcgtttt taatacccat atccttagac attaagtaaa
    15781 aagccttatt gaacacacca ctggctgtat gaactatgaa gctctggcgt tcttgaggaa
    15841 gtgcgatatg tgtattggca taggccacta cctcatcata actgatagca caatagctgc
    15901 cattattttg cgcaatacct ttgtctaaac aatctgcaga gcttccatca gaggatgggt
    15961 aatccatgaa tcgcaaagct ttgccataag aatcacgaac tattgtttca ccaatacccc
    16021 atgtgacttc attgggctgt aagtaagctt tgttatacaa ctgagggttt ttttccaaaa
    16081 gataagctct tgacgcttgt cctgccatat cggatagaga ctcattaagc gcacctgatt
    16141 gatcatgata ctcaagacca gaatgctgct ctgtaaaacc atgggtcact tcatgaccgg
    16201 ctacgtctaa agaaactaat ggataaaagt ccagtccatc tccaaatgac atggcttgcc
    16261 catcccaaaa agcattatca tagttttgcc caaaatgaac tcgcataacc aattgcattg
    16321 gcgcaccatt agaatgttgt aaggcattaa gtccatacca gtccttgtac ataccaacaa
    16381 taacatgtcc gaaataatac gcgtcattgc caggagaaaa tcctccatta attttctctt
    16441 ctatattatt gttacaaacg tattcaaaag gagtatttat ggtgttttcc caatcccatt
    16501 gagaatggag attaaccaat ttaacttttg aattttccat gacgcattgg ctgccatttt
    16561 gagtcacatc caaagcaggt aatccatctt taccatacca atattcctga actttctcat
    16621 tacctccagg ccctgtatcc aaataatttt tgatattgtt ccattgctta atcaaagccc
    16681 ctgtttgagc atcaataaca aaaaaaggcc agtctggttt accatcctgc tgcacgcttt
    16741 taaatgaaac caaataaacc agctttaatt gattattatg gtctggccga atctgtaatt
    16801 cagctgtttc ctgttgtatg ttagattggg ggctaaattg aaaataggat ttttttgcat
    16861 attccttcgc ttgttgcgca ctaatagccg gtttcgtatt aagttctata ttatccaata
    16921 aatggccgtt cacctctgca ttgtcattgg actgcactcc tgagtctgtt cctttagtaa
    16981 tcatcacttg ggcgccaata acaggtattc ctttatacag ttgctgataa cgaacaatca
    17041 tttgattatc ttcttttgtt tgatttaatt tttgcaatga attatcaata acggcagaag
    17101 aactctttaa aataatcgca ttctttggtg tttgtagtat ggggtattta ttaatgctat
    17161 tgagaggggc ttgatacaaa tctacattgt cagcagcaaa aatacttgaa gtagatatcg
    17221 acaaaataaa aaacatggtt ttttttgaca tacagaactc tcacagaata cacaatggcg
    17281 caaataatgt cacaaaatta aacattatgc aatatttatt ttcaaattct gccttgagat
    17341 gcgtaattta atgtgcatta tgtattaaaa acgatcatca gagactgggt cggggaggct
    17401 ttatagaaga agaacgctat ttgtataaac cgagtactat gaaaaatgaa atacacccct
    17461 attcgtaatt aataatttat aaccgagttc tggttgctct gcctgaagat tttatttcag
    17521 actccaagtt ggcgcgataa cctccagcca gctcttgata taaacgtaca atgcttagta
    17581 gctcttgcag tttatcctga tttaagctga tgttgatttt atcaagcgaa attttgctat
    17641 ttaaggtttc tatataactt tgaatacctg tctgatataa cctgtcgttc aaattatacg
    17701 cttttgccaa atgtttttga gccgacgcag tttgtttgag cttattggtt aaacgctcat
    17761 tttctgataa ggcattggtc gtatctctta atgctttttg taaagtatca atataattat
    17821 aataagaaac cttatttacg cctttagcct tagctactgc acctaaaacg ggcatatcaa
    17881 taatgggggc tttcaacaat tgatcattaa atttcaccgt ttttttcggt aatgcatagc
    17941 ggctatctcc agcagccaaa ccaccaatat aatctaattg tatagtaggc aaaagattac
    18001 tttctgcaac accaattcct tcatgtgaag cacgcaaacg attttcagcc acttgcatat
    18061 caggccgatt ttcaagaact gttagcggta aagaaccagg tattaattta cgatttttaa
    18121 gttgtaataa ttttttaact gtttttattt cccctggatt ttggtttagt aaataccgga
    18181 gagcattgcg acttataacg atattacgct ctgtgacctc gagctcgcct tgtatcaaat
    18241 tgacctgact atacaactct tcctgctcaa tttcagaact taatcccccc tgataaactt
    18301 tattggcaac ctttgccaac tgagttagat cattggctaa tgtctgtaaa aattctttgc
    18361 gttctatttc tgcaagatag gtgaaataac ttgccgctat ttgagaaata attgttagtt
    18421 tgagagcatc gtcttctgct tttacctgtg caagagtata ttttgccctt ttttgctctc
    18481 ttatttgatt gatgatgttt attgtatagt taggaataaa aacaatcaga acgccaggaa
    18541 aacccgttgc cggatttctt gaataaccag tcataagatc gagggtaggc acccactgat
    18601 aacgaatttt tttaagctca ccctctgccg cttcaatgct ccctcttgac atgccgaggc
    18661 ttgtattgga aatcaaccct ctttcaatca actggttgag agtggggtct ttaaaatcac
    18721 gccaccaggc tatgtaagga atattcttgc tatcgatatt tttaacattc ttgtctttta
    18781 ccgaccattt attaattact tcaacatggg gtctgtgata aggctcacta tagcatccga
    18841 ctaaaaagac tgataataaa atgattttaa ccggacgtag catgctttac tccctcacta
    18901 agatcaattg tttgctcccc tgcaacaaaa tcttttaagt gcaccaaatc tttttgaacc
    18961 tgttgaaata gttgataata atcaaacgta tgataatgtt tcgtataata agccatatat
    19021 aaggctttca ggccaatgaa aatacgatta gccaattcca caaactgatt aaattcatcc
    19081 ccgacagcat gttctgaact gattaaaaac tgggaatttg ttatgagctt attgacctca
    19141 aacaaactgt cagtcaactt gatacaagcc tggaaccatt ctgttttttt tattttaccc
    19201 tgatttaaca aacgaaccag cctgtaaaga tcatcaatca cttcagcctg agtacttttg
    19261 ataaatcgct gcatcagtcc gtaatgccta aaccaaaaat attcaaaacc tagaataata
    19321 gtaaaggcga tcaacgtgca aagggtataa tcgataataa aataggtgac tgggagcgta
    19381 tcttccgtac taaagtaacc agaaccaagt actgacacat tgaccgtaaa tacagtggga
    19441 acactgtatg ctcttcctac caggaaaaac gcgaaaaaaa ccattaaagg cataatcaga
    19501 attaacgttc gataatccag atgacctaca aaccataata gatatccggt gaataggcct
    19561 aaaagcactc ctaaaaaccg atgatacgtc ctgaatatag ttgttccatt atcaaaaccg
    19621 gcataaatca tcatgacagc aaagcccgtc cacccggccc gcggataacg caaccattcc
    19681 tggacaaaaa tagtaaacgc aaagaccgta ctgatctgaa tagagcgcag ctcaacataa
    19741 gaaaatgcct gtactcctaa ccatcggcgc ataagtaatt ccagcttaaa attaattgat
    19801 acaaaatttc caactcatgc gtttctttaa atgcaggcag ctcctggtct gagagtatca
    19861 gtggttgtct cgctttacag gattgacaaa gacgttcaag ataatgatgt aaaacaataa
    19921 tgtattcctt acgcagttgc ttttgaaaag acagcaagta agacatagtc aagattaact
    19981 caaaagataa aatggttatc tttaatatac tgtagcactt aatatttctt ggcagcattt
    20041 tggaataacg ctccatcacg ataatgcctg aaaaaatagc gatagttttg acttctccct
    20101 tacaaatttt tgcagataga gattccaggc cggtaatgac ttcgagaaaa gcacgctccc
    20161 aaatatcaaa ataatacttt ttgggaaaaa taaataatcc agcgaagatt accgccatgg
    20221 ctaccaaggt atataaagca ttattaatag caatataaaa attgctatct gcctccacac
    20281 cataaatcag ggaataggac gccaaactta gtatcaatgg aacaataaca agcatttttt
    20341 taaaatggtg aagcacgagg aaataaagag ccaaggtata gaaaaaaacg aaaaaaatga
    20401 aaagcacatt gtatacagaa attatgccga acaagaatat agagagtatg cttcctaaaa
    20461 gggtatagag taaaagcatg tacttctctt ttagggttgt tcccgccgcc tccacagtaa
    20521 aacatgtgat tggagcataa aaataataga agtacggatt gtcaatggag gaaaaaaagt
    20581 aaaataaaat catcagttcc aaaacaaaca ctgcttttaa tccgttaata cgatgctctt
    20641 tatacctatc gtaggatgca agcttatctt ttagttgaga tataaacata agcactggcg
    20701 ccaggattta aaggatagtc aggatcggga tccaatatct tgatttgtaa tggaaatcgc
    20761 tgaggaagca atagccattc attttgattt gtaacaacct gaatttgact ctttggagca
    20821 gtgctttgtc gttcagcagc ccataacgag ttgacaacaa ccccatgaaa tattttgtca
    20881 aaataataca tgcgtaaaat aatatacgct ttatcacctt gacgtacatt gcgtaagtcc
    20941 gtttcattaa aattagcctg gatataataa tttgcggtat ctataaaaga aaacaccggt
    21001 ttatggatct cgataggggt atttggagag acatacatat tatctatcac tccatcacca
    21061 ggagctctga caatggttaa atcaagatta accttggcat tatccatttt tgccttaagg
    21121 ctggcgacag tatgtctttg ttgttcaatc ttggcttgca aaaccgccac ttcctgttgc
    21181 agagcatttc ttttattact taaagtagaa acatcataac caagattttt taattcaaga
    21241 acagatactg cttctgccac gttcttattt tttttaaggc caagagcaaa ttgagctttt
    21301 tcaagctcag agtccgttgc tttaatcaaa gcaaccgttc tttcaatctg cttttgaaat
    21361 acgtctattt tttttaatcc ttccttgtaa tcagccattg cttgctgata agccaactca
    21421 taaggaaccc tgtaaacgct aaatatagga tcgttcttct ttactttttg gccattcttt
    21481 acatagattt cagtaataaa accagataca tcagcagcta caggggtcac attagtaaca
    21541 acaaacgcat tgtcagtgaa agggatcaaa taggaaaata aatgaaaaag accaataata
    21601 agagccaagc caacgacaat aatgggaaga ttaatgtaat ttttggcctt tttaaaaaac
    21661 atagggatag aataactaaa gaaaaaccgg atatgtttag ttgctatttt ataccatgtg
    21721 gcaaacttct ctttcacaat gcagcgcctc tttgatgatg actctatgat acaacatcag
    21781 tttatctctc aaagagagaa accccacgac aaggccctcc cctatttcag cattaaaact
    21841 aaaatacctc ttttttccgt tttcgaaaca atcgagaaag attgttgggt gactcatata
    21901 atcggctttt tccatcctca ggtaaataac cccattgaga ttcttctgcc agcacagcct
    21961 tggcatctga atacccagat tccattcgct tcttaatgac cactcggcca aagttgtaat
    22021 ccttcgaagc cgcttttact attcttgaac tgtaagccaa atgaaccaag gtacaatgat
    22081 gagggcttcc aatatctaac aaatgagcgc tctccttctt ttgttcatcc gttaaaatac
    22141 ggcttaattc catcattgag tttcgcattt tttgtctctg caaataattg taaatagtac
    22201 gttgggcatg ggttccatat gaaatgtctt ttcttctctc ttcaatttca ctcatgcttt
    22261 tgggaataaa cgatcgccca ccaaaacaat caattagaaa acacaaggtg ttttctgcag
    22321 gaatagcctc cagaataact tctaatgggg tgttagagtg cacaccacca tcccaataca
    22381 attctccatc gatattaact gcaggaaagc ccggaggtaa agcagcactt gccataatgt
    22441 gctccacttc tatacgataa ttgatattat taaaatagat taaatggcca ttgctgattt
    22501 gaacagcgcc aagacttaag cgaatggggc aggaatttag caattcaaaa tcaataagat
    22561 ccagcaatgt cttgcgaaga ggagtcgtat cataatagct cagagtcgtt ggatcacccc
    22621 ataaaggaat tgttccggtg caccattttg gaaaaaaaaa ctcaggctgg ccaaataata
    22681 aagcacaagc ggcacttaat ttattataaa aatctaaagt gatatcagat tcacctaaaa
    22741 aatcgaaaaa attacccggg gcaatttcat cccaaaatct ttcgagtttt tcaatacgct
    22801 tttctggagg atttcctaca ataatcgccg cttgtatggc accaatagag gttgctgcaa
    22861 tccagtccgg ttcatacccg gcttcaagaa gccctttaac cacaccaaat tgataggctc
    22921 ccaaagatcc accgccttgc agcacataag ccacacgagg gaaacgtcct tgagccttgg
    22981 agggtaagtt atcaaagcga ttggcataag gattattacc gtgcggttta ggctccactt
    23041 ccaaactcat aaaaactcct ttttcacttt agttgaccat cgcatcctgg ctgaaatgtt
    23101 gggaatactt gctgccggtt aataacactg ttcaaaatta gattgatata tgcatcgtta
    23161 ttaaataacg tgaattatgg ataaggatta tataagaagc tgtcgatatc ccgggttcgt
    23221 tcgggctgag gagatgcgta agcatcgtct cgaagccttg gcacgaaaca ttaaattcca
    23281 tacaaggctt cgagacggca taaatgcctc ctcagcccga acgattcaag ataacgtatc
    23341 cataattcac gttaaataag tttcgcagaa atcataagtg tttaatcatt cacattgtac
    23401 tcataatcct ttgagcaacc aagataatat atgaagatca ttttgaatag ttttaattat
    23461 ccggctaaaa tcaacgcaat aagcaactaa aaatgtcctg tacgaaaatc atgtattgcc
    23521 tgcgtaattt cttcttgagt attcattaca aaaggaccat agcgaacaat aggttcatga
    23581 agtttagccg cagctatcag taaacaatga tttctatttt ctttgtcttt gccttctatt
    23641 tgtattcttt caccgtttgt caggctggct aaagtgcctg cggtaatttg ctgtcctcct
    23701 gtattcacag caccttcaat gacaaaaagg acagcttgat aatcgtcagg aatcgtttgt
    23761 tgaatagttg cattggcagg gagattgatg tctagaaaca aaggtcgggt cgcaatttca
    23821 acaatgggtg aactcaatcc ttgatcaatt cttcctgcta ttactttgat ggttgcacct
    23881 gattccatga cttcaatagg tagttgatcc gatgattttt cctgataacg aggttctcgc
    23941 catttttctg cagcaggcaa atttaaccac aactgcaaac catgcaaccg gcccgttgaa
    24001 gacgaaggca tttccgaatg aataatccct tttcctgcag tcatccactg cacaccacct
    24061 gcgtttatta ctcctttgtt acctttattg tcttcatggg taatgcttcc ctccatcaga
    24121 taggtaattg tttcaaatcc gcgatgcgga tgaggcggaa acccggcgag ataatccaat
    24181 ggatctgaac tgtcaaaata gtcaaaaagc agtataggtt caaagtgatt ggttttatca
    24241 gctcctatat agcgacgtaa tttaactcct ccaccttctc gtattaatgt tccctggatt
    24301 aaccgctcta cttctattgc tttcatctta attactcatt aattactgtc ttatattaag
    24361 atagttcaaa atagtacggt ttcctaattg aattactatt atttaaacta ttggcacgtt
    24421 cagttatcaa ggatatgatg aaaatagact ctaaggatag gagcagaatt tatgaacaca
    24481 acggaacata cagagctggg caatggtttg ctcatggata aaatcgaagg caatccatac
    24541 ttgcgaatag atgagtttgg cgtgctccat ctgcgacttc aacgattcgg tgaaaacaac
    24601 ctgcctgaac ctatggagtt ggagatgtcc gctggcgaaa ttattgccat ggcgggtgac
    24661 tactttacgc aggctaattg gactatggat ttggatctgc ccaaatgcga gctttttaac
    24721 tccccggcag aattgggaaa acacctcatt cgcaagccta tagatcctaa agaagagagt
    24781 gctttaatta cagcatacaa taacctggct gcccctgatg tcactcgaaa agaaattgat
    24841 cgcatttatt ccattaataa tgctaactat gtgccctttt cgcctacgtt gaatttctat
    24901 gctcaacaac tgatgtatta ttttcgtgtc aaagactatg gagaaatgct tgttcggaac
    24961 caaacccatt ttacgccatg gtctattcga gtttatatcc tgggtcacgc tatcgcattg
    25021 agatatgcac atttatccta cgaattaaaa caattggcta ccgacagaaa ttatcaatca
    25081 gataaccctg acttgagcgc tttaaaaatc tccttacaaa ataaaaatga aactctttca
    25141 agcaataccc tgcttgattt ggcgaatcgc tatcacgccc aagcttattc tatagagcta
    25201 tttacttttc actactattc tgatcatttt gctacaggac atatgtccat gattggcgat
    25261 ttacgtgtcg tattaaaaga gcgcttcgga gtatggggca atatcctggc taataatctt
    25321 catgatgaag taaaccgtgt tggagtttat acagtgagac cctatgatcc aacccctaat
    25381 gcaacagaag ctccgtctcg agcgcgtggg gatgggaaat ttgatacctg ccttaatcaa
    25441 tttaaccgtt tagcttgcct aaacggtatg actgcatcac taaaggacat caatcaagta
    25501 ttatcaggaa gtgtaatacc ggagcaaaaa gatttcggtg gtttggtgca tatgcccgac
    25561 gttgatttta actcaagaca acatcaacca ttgctggttt taagtaaagg aaaagtatat
    25621 taccgcaaca atctcagtca aattcacata atttctcctt ctgaatacga agcactcaga
    25681 gaaagccctg aaaaacatgg gtataaagag ctaaccagta aatggactgc ctttaaatta
    25741 gttaccaaat tacgcctctt tccttatctt tatgacagct cggttatgcc tgtttctgat
    25801 gaaaagctcg ccgaaattat cgcagatgaa aaacgcagaa atccacaacg agctcccatc
    25861 cccacaccat catgccttcc ggaaagcgag ccaactgtat tcgattggcg aacaaaagcg
    25921 agctggcgta ataataaaga ctcactggat attttggatg ggttaaagaa acacagtatt
    25981 ttgggagcaa aacaaaccca ttcggccatt caagaggaag aagaggtccg gttgaatttg
    26041 ggagtctgat tgtttcctga taaactagtg gcctattgtt actatttaaa ttttagtaac
    26101 ccatttgagc tgtcttaaag ttagtaaatt ggacataagc aagtacatgc attttcttat
    26161 gtccaatttg gctaacaata ataaccgtag acaattttga ctattcaaga ggtgatcatg
    26221 aagcgcttaa tacctacttt catatacctg ctggctttag caattactcc cttctctact
    26281 catgcagaag cgcttttgaa agacaccttt ggccacaata ttcctttttc atcacttaaa
    26341 ggaaaatggg tttttatcaa ctattgggca agctggtgtg aaacctgtgt cgatgaaatc
    26401 cctgagttta atcgctttta caaaaaccat aaaaatgagg cagtagccct atacgcagtc
    26461 aattacgatg ctcttccctt accagagcaa aaattactaa ttaaaaaatt taatattaac
    26521 taccctaatt tgttaaagga tccggcccag gatttacaat taggagacat cacaggagtg
    26581 cctgtaactt ttgtatttaa cccaaagggg caattaatta aaaaattata cgggggacaa
    26641 acggcaaaaa cattagaaaa agtcattgca gagaacagaa cctcctaaga gaataaataa
    26701 aaattatatt tttggtcaat cagttaaaaa ttttattgtg aatttatcaa gtccggaatc
    26761 gattctatcg ccccgcagcc tgacttgatt attcatgaaa gcaatcgtcg tttttgatca
    26821 gccgttattc taattttcat taaccaatcg ccgttttcat ctacagactc tttaagtaca
    26881 gcgcctaatt ggtataattg ggctcgtaat tttgcctggt caggcttaat tacaatatcc
    26941 tcaattaaaa tcataccatg caattgagtt gctattgcct cttttagaag atccagacca
    27001 gcgccggttg ttgctgataa ccataccttg caagcatcct cagtataatc aatttttggt
    27061 tgccaacctt cctgcaaatc aattttatta aacacctgaa ttaccgggat attactaacc
    27121 tttaattcat ctaacacctt ttgtacttca aaaaccgtct ctcgccaatt cgggtcagaa
    27181 atatcaatga catgcaataa tagatcggct tgttgggttt cctctaaagt agcgcggaat
    27241 gcttctacca agtgatgggg aagatctcga ataaacccta ctgtatctgc taaaattgct
    27301 gctgaggacc ctggcaattc caatttacgc atcgtcggat ccagggttgc aaacaattga
    27361 tcagccacat aagtgtgttc ccctgttaaa acattaaata aagtggattt tccggcattg
    27421 gtgtaaccca ctagagaaac agtaggcatg gacgccttcc gcctggcctg cctgttttga
    27481 tcgcggctgc agcgtacttt ttccaatcgc ttattaatat aacgaatgcg ctctcttaac
    27541 aaacgccggt ccgtttccaa ttgggtttct cctgggcctc ttaaaccaat ccctcctttt
    27601 tgcctttcca aatgggtcca tccacgaata agccttgttg ataaatgttg caattgggct
    27661 agctcaactt gaagtttacc ctcaaaagta cgagctctct gagcaaaaat atcaagaatc
    27721 aagccacttc tatcgacaac tcgacatcca aataatcgct cgagattccg ctcttgagaa
    27781 ggcgatagct catgattgac taacactaaa tccgcatcga gcatgtttac caaatgagct
    27841 atttcctcag ccttaccttt ccctacataa tatttggcat cgggagtagc tcgggcacct
    27901 aatacacaat ccaacacctc agccttcgcc gaaagcgcta actcttcaaa ctcagccaat
    27961 gccttatcag catcaacccc ggggagagct aactgcacca aaacagctcg ctcaccgcct
    28021 tgtggacgtt caaacactac cctaatcccc aaacaagaaa ttgattagtc tgccacagtt
    28081 ccttcttcct caccagagct ttcctcagca ggaattttga ccattcgaga aggaacaact
    28141 gttgaaatgg cgtgtttata aaccatttgg gttatggagt ttttaagcat caccacatat
    28201 tgatcaaaag aatcgattat cccatgcaat ttaataccat tgaccaggaa cactgaaaca
    28261 ggtacctttt ccttgcgcaa ttcatttagg aaagggtctt gtagtaaatg atttttagac
    28321 attgcctact ccttgttgtt attgtaattt ttaaaggcaa aaacgggtat ctaactattt
    28381 attcgacacc ctataataaa actttagtat attttcatga cagcgtggtt tgaattcgtt
    28441 aatatagtca atcaaaatat ttttgcacat ctagaatcag gctactgatg agttaataag
    28501 gagattttaa tgacgtttac ttgcgatgaa ttaaaaggat tagagcaccc ctacaaagta
    28561 cttggcaatg gtgacgcact ggcggaaaac cgcgaagagc taaacaaatt aaccaatgat
    28621 gaaaaattag ttttagccag ccgcctcgtt ttggagtgcc ccgttaatga attaaaagat
    28681 tttgctcatg ccattgaagc agcaagaatg ccacaagatg atagcgatac ctttcattcc
    28741 tttttattcc aggcctatca ggtaaaaaaa agaatcattt ccttactcga tccgcgcaat
    28801 aaaaaccctc attccatgat attggagaag gaatttgatg gtgagctatt taataatttt
    28861 aataaattgg ctattgatgt attaactaac aatgaagtcg caattgcatt gagactggct
    28921 gaaacaactc ctgctcaaga tcgttcccga gtatctcaaa atatcaacaa tattttccca
    28981 cagtctctgt ttgccgctaa agtcggccac gctttcgccg ttcgcagaga catagagcga
    29041 ctattgctag gtgacaggcc tgatcaattc ttctcaagcc gtgaattcaa gatcgattct
    29101 tgtatcgagt ttgcaagttt attcaatgta attaatgata aagaatcttc cattgccgga
    29161 aaacttgcgc tgagaacccc tgctgaaaac cgctctgatg tagtaatgaa aattaaaggt
    29221 ttttgtgccg aggatagtga gcttgcgata aaagtccaat ctgcttttgc attaagaagg
    29281 gatatagagc gcaatctatt gggtgataat ccagaacaat ttttcaatag ccgagatttt
    29341 agcgttgatt tatgccttga gtttgcaatt ttattcccgg aattattaaa aggtcatgaa
    29401 caagctatcg gagaaaaatt agctaagctg gatgctaaag tacgctcaga tatcagtaga
    29461 aaactggaaa tgatcaatgg agcagctcac gagcaatcaa gtcctttcag aataatagcc
    29521 agtgccatgg tgcctaaaga agagccttgc ctgacaaagc ccactcccgt tgtcttgcca
    29581 tctactgaag aaattatacc aaagcaagaa tcaccaacca gtttacgcaa caggaacgtt
    29641 ttatttaaag aacaaacaga agagcacaag aagaaaaact ctcagatgga acccattgcc
    29701 tcagattctg aagaagaaga agagaaacag aattgctgga ctagtttttg tggattattc
    29761 ggcaagtaaa gcagccatat cttttgccct tacttggtta agggcaactt tacacttttt
    29821 tacccattct ttacacatca tgtagcaata attcatgtta atatttctca taaattcatt
    29881 gacttattgc aatctgctcg agttcaagcc ataaattaag aattgaacgt cgctttaaac
    29941 ttaataaatg attcgaacag aaagactatt gaataagttt gcttttttag ctttaaaaca
    30001 atagaagccc cttacctcac aaaaaatcaa ggaaaaacga agtgtgctgt cctaaatgaa
    30061 aggaaagatt cgcctctaat caatagtcgc gagccttcac cactttcaga atagctgcca
    30121 ttctgaaaat ttattagtaa ttagtaacag ccaaaatgag cagtgaattg aaattaatgc
    30181 tagtacaaag agagcttttt ttgataacat agccagattt ttaatctggg ttgatgattt
    30241 aacttaagca agagtaagcc attcgaattc gagataaaaa ttacttaaca tcatttattg
    30301 ggaactctaa tgaaagaaaa caaactaacg gtcgcactgg ttcaggaaca atggtatgaa
    30361 aatcctaaag agcatcaaga taaactggct tctggaatat tttcagctac ccagcaagga
    30421 gcaaaactcg tttgtttaca agagcttacc ttaagccctt atttctgtac acgtagtgac
    30481 gtcgatcccg ccccttatat ggaggatatc gccactggtc ccactgctca atttgttagc
    30541 caaatggcaa agtcgaatca aattcatatt accgcatcgc tttttgaaaa agctggttac
    30601 aataccgcgg ttgcttttaa tccccaagga gaattgattg ctgttaccag aaagcaacat
    30661 atccccagtg gcgaaaaata tcatgaaaac ttttacttca agccgggcga ttccaattac
    30721 cctgtccata ccattgctgg tcactacttg ggattaccaa catgctatga ccaatggttc
    30781 cccgaattat caagaattta tggattaaaa ggagctgaaa ttctggtcta tcccacagcg
    30841 attggagggg agccgactgc gccaggattt gatagccaac ccatgtggca aaaagttatg
    30901 gtagcacaag gaattatgag caacaccttc attattgcgg tcaaccggat tgggtgtgaa
    30961 gatggtttaa gtttttacgg aagcagcttt atcagtaccc ctatgggcga aattttagtt
    31021 caggcaccgc gtaatgaacc tgccgtattg gttgctgaac tggattttag ccaacgtgaa
    31081 ttgtggggac gattattccc atttccccaa caacgagagc ctgaaactta tcatgaatta
    31141 gtcaaatccc gttaatcctc tttggaacgt tagaatgaac aataatactc tatcagatga
    31201 caatctttac aacatagaaa attggggcga gggatacttc cgaattaact ccaagggaaa
    31261 tattgaaatc agtaaaaaac ccggcaaacc aggtgttgaa ctgcaaacta tagttgatgc
    31321 agctgaccgt gccggcttgc atttaccttt attgatccgt tgtagtgata ttcttcatga
    31381 tagagtccat agaatatacc aggcgttcac ccaggctcga gaagacactt attatgccgg
    31441 taattatcag ctggtttatc caattaaagt aaaccaggaa caaagtgtgg tacgggaatt
    31501 gttaaaatcg cctgataatc agataggtct ggaggctggc agcaaacctg aattgatggc
    31561 ggttataggt atgctgggtg accatcagag tactatagtt tgcaacggct ataaagacac
    31621 aagttatatt cgtacagcgc ttattgctca acaaatgggt cataaagttt ttattgttat
    31681 tgaaaaaaaa tctgagttgg acattatttt aaaggaatct gcacgccttc aaatcaaacc
    31741 aacattaggt gtgcgaattc ggctggtcag caaaagcgct ggcaagtggg aaaataccgg
    31801 cggggttaaa tcaaagtttg gactaaatgc caaacaggta cttgaactga ttttacaatt
    31861 aaaagccaat gatatgctgg attgtttgca gctaatgcac tgccatttgg gttcgcaaat
    31921 tgccaacatc catgacatac gccattgcat gcaggaagtg gcgaggtact atattgagct
    31981 acgtcaactg aatgctccga taggtacgat tgatgtgggt ggaggtctga gtgtcgatta
    32041 tgaggggact cgatccaata aagggtgctc aatgaactac agtcttgttg agtacgctac
    32101 gcacatcatg ttggctttaa aacacttatg tcaagaagct gagataccag agcctgatct
    32161 tatctccgaa tcaggacgag cattaacagc ccatcatgct gtattaattt ctaacattac
    32221 ggatgtagag atcatcaata aagcgcccgt tcttcctgaa atatcggaag atgattcgca
    32281 tgtcattcga gatatttacg atacttatca agccataaca gatagctcgc ccactgaaat
    32341 atataattac gcagctcatt cgcttaatga agcgcattcc cttttcaagc atggggtaat
    32401 tagcctgcag gaaaaggcca aggtagaagc tttttatact aatatttgta tggaaataaa
    32461 agataaactg gatgaagaaa atccaagtga aaaagctttg ctaatgagaa tcaatgaatg
    32521 tatggctgcc aagatatttt gtaatatctc ttttttccaa tctattcctg atgcttgggc
    32581 tattggtcag attttcccgg tagcccctat atctcagctt aatagaacac ttagtatgca
    32641 tagtatccta caggatttaa cctgcgattc agatggtaca attaagcaat accctggaag
    32701 ttcctgcgtt acaaccacat tgatgctccc ttcatacgat atcaataacc cttataacat
    32761 cggatttttc ctggttggtg cctatcagga aatattgggt aatctgcata acttgtttgg
    32821 agataccaat tctctagatg taaagctttc tgatgatgga caatttgagc tggatgattt
    32881 agtcagcggc gacaccgtaa caaacgtatt gaatttagcc cattttgata ccaaaaagtt
    32941 aattcaatct tatgaaaagc aattaattaa atctgaactt cctaaggaaa aggcactctc
    33001 ttacttaaat gaattaagaa gtattttttc tcaattaact taccttgatg gaaatatagc
    33061 acagtgaata tgaatacagc acccaaacaa tatggttttt atatgcctgc tgaatggtat
    33121 cctcatgaac gatgctggat ggcatggccc tgtcaccatg acacctggtc caaaataggg
    33181 ctagacaagg ctaaaatggc ctatgcacgt gttgcaaaag ctatagctca atttgagccc
    33241 gttactttat tagtaaatcc aggagatgaa gattccgcag aaaatttatg caaagggcat
    33301 aatattgaaa ttatctcttt gccaattaac gattcatgga cccgcgatac gggcgctacc
    33361 tttttaatta ataatgaaag gcagcttgcc ggagttgact ggatacataa tgcttggggt
    33421 ggaaattacg ctgactgttc tttggataat ttactagctt ctcatctaat aaaatacact
    33481 gaagctcaat actttcatgc gccattggtc atggaaggcg gttcctttca tgtagatggt
    33541 gaggggacta ttttgacaag caaagagtgt ttattaaatt ccaatcgaaa tccacattta
    33601 tctcaacagg aaatcgaaca gtaccttatt aattttctgg gtgctgagcg tattatttgg
    33661 ctgaacatgg gattgatagg tgatgaaaca gatggtcatg ttgacgaaat agcaacattt
    33721 attgctcctg gaaaggtgtt atgccttatt accaaggata aggaagatcc taactaccat
    33781 cgcttgcagg aaaattttga aattctcaaa tcatctaagg atgccagagg acgaacattt
    33841 gaggtctata ctgttgagca accacctgca acttacctta atggagaaag gctcactctc
    33901 tcgtacatta atttttacat ggctaatcaa ggtattgtta tgcctgcttt tggatatgaa
    33961 tcttttgaca gattagccta tcaattattc gtacaaatat ttccaggata tcaaattaca
    34021 caaattgatg cgctcgatgt attttctggt ggtgggggta ttcattgtat tacacaacaa
    34081 caacctaaat cttataaatt agtagggtaa ttacgaagac aatgccactt cttaattgag
    34141 aaatttctta catattactg ctcattatac tcgatgacat atactgccaa ttggttatac
    34201 taaaagcata cagggatgac atggatatgt ctgacaggaa gtcaactaag ggaccagtac
    34261 aaaggatgta cttcgctgca aggacgcagc acttattctc caatttacca gtatacctct
    34321 ttaaattttt ctattaagca atcaaaaggc tttttttgaa cacatcatcg tagtaggaaa
    34381 ataagttaag tcattggata aatcagctat aagctagaga ggcgtttgcc actagcgagg
    34441 caaacgtcac tccagtttat caatactatc tgtttatata tcaatgtttt cagcgtctaa
    34501 agcattcgtt tcaataaaat ctcttcttgg ttctacattg tcgcccatta aggtggtaaa
    34561 gatttcatct gcagctacgg catcttcaat gctcacttgc aacattcgtc taacctcggg
    34621 atccatggtt gtttcccata actgctcagg attcatttcc ccaagacctt tatagcgttg
    34681 aattgtttgt cctttttttg cttcatccat tagccaatta agtacttgtt caaaattttc
    34741 cacaggcaag gatttatctc cacgctgaat ataggcacct tcctctatta aattcgacaa
    34801 tttatgacct aaatcaacca tttctttgta atctttagac aagaaaaatt caacttgcat
    34861 tggaatatca ttttccaagc catgctgata cactgtaatt tttggagtgt attgattacc
    34921 ttctgtaagt ttttggatat ctactttgta ttgttctgtt ttactgtcta cttgttgtaa
    34981 gcttaagtgt aattgtctgc accagctttc aatttttgac agattgttaa aatcttcttg
    35041 gtgtagcatt ggcatataag ccactctttt tagtatatcg ctggggtatc ttctcttgta
    35101 ccgctttata atcttctcaa ctcgtctgaa atgttgaacc aattcttcca gagatttggc
    35161 agatatgggc ggagcctctt tggcgggata aaatgtcgcc ccatccaacg cgcactgagt
    35221 tagataatca aataacgctt catcatcttt aacatattgc tcctgttttc cacgctttac
    35281 tttatataaa ggtggctgtg caatataaat atatcctcgc tccaatagct ctctggtttg
    35341 tctatagaaa aaggtaagaa gtaaagttct tatgtgggac ccatccacat cagcatctgt
    35401 cataatgatg attctatgat aacgtacttt atctggatca tattcttcag gaccaatccc
    35461 acagcctaat gcggtaatca aagtggctac ttcttgagaa gaaagcatct tgtcaaaccg
    35521 ggctttttca acattaagaa tcttgccttt aagaggtaaa atagcctgga attttctgtc
    35581 tcgcgcttgt tttgctgagc ctcctgcaga gtcaccctca actaaataca actcggaaag
    35641 cgatgggtct ttttcttggc aatcagctaa tttacctggt aaaccggcga tatccaaagc
    35701 ccctttacgt cgagtcattt cacgagcacg ccgcgctgcc tctctagctc ttgctgcatc
    35761 aataattttt gctacgatgg attttgctgc agtgggattt tctaaaagaa aatcgttaaa
    35821 tttttcagca acacaggatt ctactgctgg ttttacttca gatgaaacca atttatcttt
    35881 cgtttgcgat gaaaacttgg gatcagggac ttttacagaa agaacagctg ttaatccttc
    35941 acgagcatca tcacctgttg gagatacttt tgccttttta gcataccctt cgttttctat
    36001 gtaattattt aaagtgcgag ttaatgctgc tctaaagccg gccatatgtg ttccaccatc
    36061 cctttgcggg atgttgttag taaagcaaaa tatggtttct tgatagccat cattccattg
    36121 cattgccagt tcaatgacaa taccgtcttt ttccgatgtc attgagaaaa caacggggtg
    36181 gactggtgtt ttatttttat ttaagtgctc aacaaaggct ttgataccgc cttcgtagtg
    36241 aaacgtgtct tttttctgtg ttctttcatc aaataagtga atacaaacac ctgaattcag
    36301 gtatgacaat tcacgtaatc ttttagctag aatgtcgtaa tggaactcaa tattagaaaa
    36361 ggtttcagcg cttggtttaa accatatttg tgtgccagta gagttggatt ctccagtttc
    36421 agcaatagga gattcaggaa cgccgtgtct gtagtgttgc tcatacacct ttccatgcct
    36481 acgcactagc aaatgcaact cttctgaaag ggcattcacc acagataccc ctactccatg
    36541 cagaccgcct gataccttgt aagagttatc atcaaattta cctccggcat gcaggactgt
    36601 cataatgact tcagcagctg attttccttc ttccttatga atatcaacag gaataccacg
    36661 accatcatcc ttaacggtaa ttgattcatc gctatgaatg gtaacgaaaa tttccttgca
    36721 ataacctgcc agggattcat ctattgaatt atctacaacc tcaaaaacca tatgatgcaa
    36781 accggttcca tcatctgtat ccccaatgta catgcccggt ctttttctta ctgcgtctaa
    36841 accttttagg actttaatat tatttgaatc ataactggcg tctacgctca tcagctgccc
    36901 tcttgccgta ttatgctatt aacaatgagt ctattatacc atcttttgta tctggtttat
    36961 atcttaagta gagaacttta cgcttgtgta ttctgaatta ttgacgttga tttttttgaa
    37021 tatcattttg tttatttgat tttcatacca tgacatttga ggagccattt gtagaatggc
    37081 atatgtttac ctttagaatt ttatattgca tttgcattaa ggtgttattg tttaataacg
    37141 agaacttttc attccattaa tttgtgttcc acgtgaaaca ttctatatta taaaacttta
    37201 attgcgccac agtttacttc aaacaaacca gagtcaattg gcaagatatc gtaattattg
    37261 ttgttaatta aatttgttat aacaaactga cccttctgct gtgtcagata cttaattaaa
    37321 tttcgctggt gatagtcatc taactcagca gccaaatcat caataaggta taagcaatcc
    37381 ttatctaata gctgaccttg agccaattta agtgcgatta gaatgatttt ctgttgtcca
    37441 cgagagagtg tatgctttac tctatattgc tcaatgttaa ttatcaaatc agcttggtgg
    37501 ggtccatatt gcgtatatag cttattttta tcactatcaa aggatttctg aagcagttcc
    37561 tccatgttct gccctgcatt ttttctatcc caccctttgt aatactcaat tgtgcaggat
    37621 atattggtta aatcacttaa aacttggtag aatttaggtt gccattgcaa aaaatactca
    37681 tttctagctt tatccagttg atttgccaat tgacttagct gttggtccca aggaataaaa
    37741 tgttcataag tagctctgga ttttaataat gcatttcgtt ggctaagaat gcgcttgtaa
    37801 tccttccaaa ttttcaaata atcatgtttc acgtgaaaca atccccaatc aagtaagctt
    37861 ctccttaccg aggggccagc atcaataatt tgaaaaatat cagagtaaat aacctggcaa
    37921 ggaagcgcat aggctagttg acttgtagta caacaaaact ggttattcaa tttaatttgt
    37981 gtgccatctg ttatagattt ctgaacacta atagtgcttt cgtcgtaagt atgggcaaaa
    38041 acattaagtt ggttttgacc ataagaaata attggagcta cttcacgaga ccgaaaagaa
    38101 tgggcgcagc tcaacatgta aagagcctct aataacgatg ttttaccact gccattaggt
    38161 cctgtaatac aattaaaatt gggatttagg attaaactag tcgaagcgat attccgaaaa
    38221 ttatgaattc taacttcaga taaaatcata ttttcatagg cataataatg tattgataat
    38281 tatcgttgac taatgactca attaaaatac tgctttcagt attagacatt gataacctga
    38341 tttgaccatc agaaaagtga gctaaaacat ccagcagata ggttgcatta agtccgattt
    38401 tgagttcatc accttgtgtt tcggcactca atgtttcaat tgcctcttct tgctcattat
    38461 tattggcaat taaagttaac tgacctggtt gtagatgcaa caagacagcc tttgactttt
    38521 catgagctaa aatgacaata cgagataatg cacgcttgag cgtagcgcaa tcaataacaa
    38581 tatatttatc ctgttctctt ggtatagctt tagtataagg tggaaagcgt gcttcgatta
    38641 atttagacaa aaatacaaat tggttagtaa tcaacttaat atgagatttt cccgcagcca
    38701 acaaaacttg ttcgtcatta atacaattca atagtcttaa taattcttgc accccttttt
    38761 tgggtataag taattttgtt tgagtaatat tattacagga atatcgcgat atagccattc
    38821 tgtgcccatc agtagcaacc gctgaaataa tattactgtc aaactccagc aataatccat
    38881 tcaaaaatat acgaacatcc tgctgagaca tagcaaaatg agtagattgc aataactgca
    38941 acaaaaccaa acgcgggata gtcaactcga catcattgca ttcatcttcg ctactgggat
    39001 aattctcagc aggcaaagtg gccagtttaa atgtacttcg gttttgtttt atcgtcacta
    39061 aagctttatc aaaaacgata gatggcgaag ggttttcatc caatgaccgg ataatatcaa
    39121 tgaatttctt ggctggtact gtaatagaac cactagtatt attggaatca catgccactt
    39181 gtgcggacat ttcaatttcc aaatctgtag ccgtaatata taaaagtcca tccgacagtt
    39241 ttaataaaaa atgggacaaa atagccaaag actgcttctt atctacggct ccggaaacag
    39301 taagcaatgg agtaagcaga tcttcttttt taatggttag ctcaaacaag atagactccg
    39361 tatcaagaag ataatattcg cattaaattt ttatagtctt cagcaaaatc gctatcatcc
    39421 tggattaatt ctttaacctt ccgacaggca tgtatgactg tcgtatgatc cttaccaccg
    39481 aaatgatcac cgatttcagg taaactatga ttagtcaatt ctttactcaa agccattgcc
    39541 atttgccttg gtcttgcaat agaccggctt ctgcgttttg aaagtaaatc agcgaccttt
    39601 actttataat attcagctac cgttttttgg atattttcta tagtaactaa cttatcttgt
    39661 aaagctagaa gatcacgtaa tgcttcatgt acaaattcaa tagttatcgg cttaccagta
    39721 aagtgagcat tggctataac tcgacgcaag gcaccttcaa gttctctgac attcgaacgt
    39781 atacgtttgg ctataaaaaa ggctacttca taaggcaatt caatattaga ttgctcagct
    39841 ttactaatta agattgcgac gcgcgtttct aactctggag gctccactgc tacagtcaat
    39901 ccccaaccaa atcgagattt taaacgctct tccatccctt ctatttcctt aggatatcga
    39961 tcactggtca aaatgatttg ctgttgtcct tccagtaagg cgttaaaagt atgaaaaaat
    40021 tcctcttgtg acctgtcctt ccccgcaaaa aactgaatat catctataag taaagcattt
    40081 aaagaacgat aaaatcgttt aaattcatta atagaatttg tttgcaatgc tttaaccata
    40141 tctgcaacaa aacgttctga atgcaaatac aatactttag cctcaggatt attctttaaa
    40201 atactattac caatggcatg catgagatgt gttttaccta agcccactcc cccataaata
    40261 aataagggat tataagcatc cccagggcgc tcggctacct gcatggacgc agctctcgcc
    40321 agttgatttg aatttccttc gacaaagctg tcaaaaacaa attttttatt taagtgactg
    40381 cttttatagt caactgcttt tttaggagcg gattttagag gtgctgtact agatgaagtg
    40441 acaattgttt cagcaggcgt atcaacagct tcgacaggct tactacctac ttcaatagaa
    40501 atagctttga tgtcatcgcc ggagaactgt ttaatcaact cttcaatacg agaaaaaaaa
    40561 tgttttctaa cccaatcaac aacgaaacga ttaggcgcta ataagattaa acgctgctcg
    40621 tccatgtaag cttgtaaagg gcgcaaccaa gtattaaatt gctgagcaga aaattcatct
    40681 tgcaacaaac ctaaacattt ttgccaagca gtagttgaca caaaataact cctaatttaa
    40741 acaagggtgg taaaagaata cttaaaacta tctatccaca gtattaaata ttgccattga
    40801 aagtacccg
//
LOCUS       DATNSN010000011        39687 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.011, whole genome shotgun sequence.
ACCESSION   DATNSN010000011 DATNSN010000000
VERSION     DATNSN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39687
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.011"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(60..1250)
                     /locus_tag="VAH76_000474"
     CDS             complement(60..1250)
                     /locus_tag="VAH76_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HEO1385127.1"
                     /translation="MRSRFIGGILLIVGTSIGGGMLALPVANAATGFWQSSLFLLICW
                     AFMTFGAFFILEANLYLSRGKHMVSMAKATLGDYGLLTAWISYLFLLYTLLSAYISGG
                     ADVLNSLLSKVNWHLSDWQASSLFTLIFGLVVYGGIHSVDVANRALMFGKLAVYCILV
                     ILIAPHIEFHYFQHGDYRQIGGAIMILITSFGFAIIVPNLRDYFDDDIKTLKKVIFIG
                     SLIPLICYIAWDAVIIGSLPSEGTSGLESLMQNPRTTSALASLLTDKVENTTISALFN
                     FFTSICMLTAFLGVSLCLFSFLADGLKLRERGRHGIGLFVLTFLPPLLIVIYYPGAYI
                     YALRYAGIFCIILLLLLPALMCYFGRKRYKSPFWVPGGKISQFAVIIISVVLLAHEFI
                     ALFK"
     gene            complement(1261..1953)
                     /gene="cmpA"
                     /locus_tag="VAH76_000475"
     CDS             complement(1261..1953)
                     /gene="cmpA"
                     /locus_tag="VAH76_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-OmpA-like family protein CmpA"
                     /protein_id="HEO1385128.1"
                     /translation="MSRKLAKTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVD
                     TGIGAVAGVIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGD
                     TRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKR
                     KLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSP
                     AQPPQPQMAYVK"
     gene            2184..2444
                     /locus_tag="VAH76_000476"
     CDS             2184..2444
                     /locus_tag="VAH76_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385129.1"
                     /translation="MKIPAQLKNIGGNIPENDIALQVSYFIKSQNDELLPQSDAYIAP
                     ASGKIDVSFGNNLTRNKDVFFAVISTADKKIILLNLKDDNWV"
     gene            2837..3682
                     /locus_tag="VAH76_000477"
     CDS             2837..3682
                     /locus_tag="VAH76_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385130.1"
                     /translation="MSKKAIAISIVIALFFITAIWFAYSFVKQEENQNWLTLYGNIDI
                     RDVSLSFRVSGRILSMDVDEGAHVKKGQVLARLDKDTFIANLEMAKAQLEQAIANQKN
                     ASRTYKRREQLVDDGAVSKALFDDAVAMKDSSSAEVSVAKANLEKAQISLNDTEIHAP
                     THGTILTRIQEPGAIVNVAEPVYTLAIDNPVWVRTYIDEPFLGRIYPGQDALVYTDSN
                     PEKPYKGHIGFISPQAEFTPKIVETTQLRTDLVYRIRVVIDQPDNGLRQGMPVTVKIH
                     LKANS"
     gene            3685..5427
                     /locus_tag="VAH76_000478"
     CDS             3685..5427
                     /locus_tag="VAH76_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385131.1"
                     /translation="MNSSKPLVSINGVSKCFPGNTDPALDNVSATIFEGQITGLVGPD
                     GAGKSTLMRLICSLMLPTQGTITVDSLNTRTDSEKIHAITGYMPQKFGLYEDLTIIEN
                     LRLYAELRNLYGEKREEQFRTLLKFTALEPFQKRLAGNLSGGMKQKLGLACALMGEPK
                     LLLLDEPSVGVDPISRRELWTMVQGLLGRGMGVVWSTAYLDEAEKCDQILLLNAGKPI
                     YHGKPGDFLQKTKDRTFQICGVSLLNRRQALMNALNTPEVIDGVIQGKNIRIVISDKK
                     IKPSLDGITREPGVHFQEVESRFEDAFIDSLKTKISGRSLLAEHIAEKPHSDKPIIEA
                     RKLTKRFGSFTAVSSNEFTIKRGEIFGLLGPNGAGKSTTFKMMCGLLQPTQGQAFVMG
                     LDLKTSSSEARSRIGYMAQKFSLYSHLDALQNMRFFSGLYGLSGERQEAQINSMIEIF
                     DLKKHLKQNSGDLPLGFKQRLALACAVMHEPDVLFLDEPTSGVDPLTRREFWTHINGM
                     VNKGVTVMVTTHFMDEAEYCDRIALIYRGKNIATGTPDDLKDKVRNSTLPNPTLEDAF
                     IELIRLDELREPVH"
     gene            5445..6566
                     /locus_tag="VAH76_000479"
     CDS             5445..6566
                     /locus_tag="VAH76_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385132.1"
                     /translation="MNIRYLKALVIKETLQIIRDPSTILIAFVLPLILLFIFGYGVNL
                     DTDQVKFGLALESYDSSTVSLSQSFSSSRFLDVSISTNRQAFRYGLVNGTIRGFIDIP
                     QRFTADSLSGNPKPALQNIVDGSYPQVAGFAENYALGALQTWISSQAYEHGQMTLKSG
                     INLEPRYWYNQELKSRNFLIPGSIVIVMTLIGTLLTSLVIAREWERGTMEAMMATPIS
                     IVDIILGKLIPYFVLGMGSMFLCTIIATWYYGVPFQGTLLALTLVSAVFLVAALGQGL
                     LISTLARDQFVASQLALMSAFLPSFILSGFIFEINSMPKWIQLLTHLFAARYFVTSLQ
                     TLFLTGNIWSLLIRCLFAIALIGFAFFFITAHKTRKRLD"
     gene            6566..7690
                     /locus_tag="VAH76_000480"
     CDS             6566..7690
                     /locus_tag="VAH76_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385133.1"
                     /translation="MAKLSPATRIFALVIKEILTLFKDPKGRIVLIAPPLLQLLIFSF
                     AATMEIKNISIGILNQDSGKHGYEVIQRFIGSPTFSKVFFIQKQSDIKPIIDNQEAMV
                     VMTIPQDFSRKIESNSQANIQLILDGRRSNATQIVNGYISSIIENYNQEIQKTHGAIM
                     MPPVMVTRSWFNENLLYLWFTVPSLICILSMLISLVVTALSVARERELGTFDQLLVSP
                     LTPYEILIGKTIPAIIIGLAEGLLMWVAAVWLFDVPFYGSPLLMVYILFIFIMSTVGV
                     GLFISSMCKTQQQAILGVFIYMVPVVTLSGYASPVENMPEWLQDLTWFNPLKFTLIAV
                     IGIFLKGMSALEVFITTWPLLLVGAVTLFIAGWFFKQRME"
     gene            7699..9138
                     /locus_tag="VAH76_000481"
     CDS             7699..9138
                     /locus_tag="VAH76_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEO1385134.1"
                     /translation="MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKIS
                     SSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAEARIRQARAMQQISLANFYPQIN
                     GSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
                     KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYK
                     AGVSSEVDVSLAEGQLKNTESVLPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSL
                     PKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFFPQFNLSVSNLAFQS
                     TSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQE
                     AQSALVRYSQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAET
                     QEVNSEVSSLTALISLYKALGGGWENFEA"
     gene            complement(9470..11251)
                     /locus_tag="VAH76_000482"
     CDS             complement(9470..11251)
                     /locus_tag="VAH76_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEO1385135.1"
                     /translation="MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPS
                     PEFLEFVKENTPLANKLRDKWLYELARIKDWEDYNKYYQPTNDINLRCFAQIAKFNLG
                     LYKEAIAGSIPIWLSGESRPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLAR
                     YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKL
                     WQEKKTNIMLNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFA
                     LKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFL
                     ASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPK
                     EEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPEL
                     VYAIIRQESGFREDVTSSAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINI
                     GIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYL
                     KNVMAFYIVYQYRLNQQPDMNRFLTPL"
     gene            complement(11293..11946)
                     /gene="rpe"
                     /locus_tag="VAH76_000483"
     CDS             complement(11293..11946)
                     /gene="rpe"
                     /locus_tag="VAH76_000483"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO1385136.1"
                     /translation="MTFQILPSLLSADMTRLGEEVDAVMKAGADFIHFDVMDNHYVPN
                     LTFGPAFCDALIKKFPNLPIDVHLMTTPVDTLIEAFAKAGAKRISIHPDATIHLDRSL
                     QLINNLGCEAGLVLNPATSIDTLTWCAHNLKFVLVMTVNPGFGGQKLIPEVIPKLSKI
                     RQQHPNLDLCVDGGVTPQNIASLAHAGANQFVAGSAIFNSSNYSETIKHMREQLASI"
     gene            complement(12162..12626)
                     /locus_tag="VAH76_000484"
     CDS             complement(12162..12626)
                     /locus_tag="VAH76_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEO1385137.1"
                     /translation="MKALIAKTYLLADKIFDFLAPIFILLTRLYLAQVFFLSGLTKIS
                     NWQATLSLFQNEYMVPVMSPTLAAYLGTACELGFPVLITLGLGSRLVLFGLFLFNLFA
                     VFSYQFLWTPDGAVGLTDHMQWGIMILMLLCTGPGKLSIDYLIRRFYLKLDN"
     gene            complement(12623..13402)
                     /locus_tag="VAH76_000485"
     CDS             complement(12623..13402)
                     /locus_tag="VAH76_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEO1385138.1"
                     /translation="MRNTLLETIQKEWIAQFKEEALSSPLKNRLARSNFDGISVYMES
                     HHATLFNHLKSTFPLCMRLVGENFWYALLHRYINNCESTQYDINQYGDSLPQFIANFE
                     PAKELPYLQQLCAIEWHWHTLKLLSPVNPFDFEAFSSIDEISFQSLCFHLTPNLKILC
                     SEYPIYQIWAMNYFQQDKQIQISGCPEKVVIYQHNDLPCIMLISEEEYIFLELLSSGN
                     QTPWQKVVTELSKWISEEKIGDTLTKALRYQWINHFSLEAI"
     gene            complement(13395..14264)
                     /locus_tag="VAH76_000486"
     CDS             complement(13395..14264)
                     /locus_tag="VAH76_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEO1385139.1"
                     /translation="MYSNHSTDQAAPYSGCSGIGLRLEHIDDILKEQPTVDYFEVLAD
                     NYMKQSGAQFKQLLKIAEFYPMSLHSVGLSIATSSEPDYQYLQQIKNLAHCLNSKLIS
                     DHLCWTHANQFFTHELIPFPYTEETLSFIIEKTNCIQEYLNQPIMYENVSRYVTYRQN
                     TLSEAEFLNELSTATGCGILLDINNLYVNWYNHGDDPDKYLHSINTKNVWQMHLGGFS
                     KQEGYLLDSHSDKVYQDVWQLYEKAQNLFINTPTVIEWDNDLPDFSLLYDEMCKAKAI
                     IKRVAERSMNHYA"
     gene            complement(14270..14524)
                     /locus_tag="VAH76_000487"
     CDS             complement(14270..14524)
                     /locus_tag="VAH76_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEO1385140.1"
                     /translation="MNNISKITMAAVMAAGLSMATIPANADTPNDMEKCQGVAKAGKN
                     DCGTAQHACAGQSKTDGSTTDWIYLPKGTCEKLVNGTVKS"
     gene            14862..15887
                     /locus_tag="VAH76_000488"
     CDS             14862..15887
                     /locus_tag="VAH76_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1385141.1"
                     /translation="MAKDTKIINLALQGGGAHGALAWGIIDRLLEDGRIHFDAISATS
                     AGAMNAAVLAYGFATGGKEGAREALHQFWKMVSDAGQIYNPLKKTPLEELLGIGIENS
                     MSFFMFDLMTKILSPYQFNPLNFNPLKEILEQIVDFEKIKSCKSIKIFISATNVRTGK
                     INVFDNKKISADAVMASACLPFMFQAVNIGEDYFWDGGYMGNPAIFPLIYNSECRDIL
                     ILHINPIYREHVPDTAAEILNRVNEISFNSSLMREMRAIAFVTKLIDSGWIKDEYKNN
                     LRKLYLHAIRADVSMESSSVASKLNPEWSFISHLYEEGRKKGEEWLKSNFKHLGKMTS
                     IDLDEYL"
     gene            16003..18218
                     /locus_tag="VAH76_000489"
                     /pseudo
     CDS             16003..18218
                     /locus_tag="VAH76_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            18260..19024
                     /locus_tag="VAH76_000490"
     CDS             18260..19024
                     /locus_tag="VAH76_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEO1385142.1"
                     /translation="MDKYLSANSLEGVIDNEFSMPAPRWLNTYPAGPYRFINREFFII
                     AYETDPDLLQAILPPDMELLEPVVKFEFIRMPDSTGFGDYTESGQVVPVRYKGEEGGF
                     TISMFLDCHAPIAGGREIWGFPKKLAKPKLFVEEDTLIGILKYGSIDIAIATMGYKHR
                     PLDTEKVLESVKKPVFLLKNIPNVDGTPLVNQLTKTYLTDITVKGAWTGPGSLELHPH
                     ALAPISNLYIKKIVSVSHFITDLTLPYGEVVADYLA"
     gene            19062..19334
                     /locus_tag="VAH76_000491"
     CDS             19062..19334
                     /locus_tag="VAH76_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEO1385143.1"
                     /translation="MNKRPIQNTAVIAAALGAALASIYTYTDWLSSDEITVKRERCYN
                     VARAGKNDCATSQHSCAAQSTADRDPEAFIMLPKGLCERIVGGRSG"
     gene            19327..20640
                     /locus_tag="VAH76_000492"
     CDS             19327..20640
                     /locus_tag="VAH76_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEO1385144.1"
                     /translation="MDSYSKLPLRIRKEIEAQQYTGELLVAGVQLGVIVLLSIINIFS
                     SAGYSPGAPVQSSSLGLWLFAILILLRLWFVYTNQLNPIFLGFFIVAEMALLLFIIWT
                     YYLQFETTPTINLKNPHINYVYVLIALRALRFEPLWVILSGLTAALGWGIIAWQTLSS
                     SGMNVITWDYVTFASTRSVYLGAVFDVILSVLLVTTIIALVLTRAKHTLFQAVEQTSA
                     AKDLSRFFDTSVVEKITTSEKSLQAGYGELRQAAIMFIDMRGFTKASVKLSPSELIGL
                     LAEYQRLLVPIIQKNNGSIDKFMGDGILASFGAVTPSNTYAADALRTVDEIVASVDVW
                     SENRRKKEQIVVGVGIGLASGEVVVGVIGYENRLEYTVIGEAANLAAKLEKHNKEEKT
                     QALTTRDTFLEAVKQGYEDKITKKELNARNVGGVGEPLDLVVLAK"
     gene            complement(20660..21475)
                     /locus_tag="VAH76_000493"
     CDS             complement(20660..21475)
                     /locus_tag="VAH76_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEO1385145.1"
                     /translation="MPTIKQMQEFFLNTVLNQQDIAIFEYFCPDEITNEFRFDIYRNT
                     IFQNLRHSLEMTFPAIWKLVGKECADGLALHFIQDKNNLPTTHCLDDWGEKFPLFLKN
                     NPTVSHLLYLKDIAEIEWLKHLSYCSRDCLVFDPQKLQKHLNDRVEKLILLFNPSVFL
                     YSSSYYLKNIFDLIENPTETGSIDFQQIPSYAVISRQKNQTVIHWITHDLFHFLCHIK
                     AGASLGFAYENTLENNPDFDLLTALQFLLRNELLEDSFIHSNYHLTHANNCPE"
     gene            complement(21462..22295)
                     /locus_tag="VAH76_000494"
     CDS             complement(21462..22295)
                     /locus_tag="VAH76_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEO1385146.1"
                     /translation="MHKIINGAMTGIGLRAPHYAQILEEKPDIDWLEVHTENFLVKGG
                     PLLELLDSISQHYPLSFHGVGLSLGSAEGVCIAHLNRIKTLIDRFQPALLSDHLSWST
                     IGNTYLPDLLPIPYNEESLTIISNNIDKVQNYLKRTLLIENPSSYLEYQTSSYSEPDF
                     LAEIIKRTEARLLLDINNIYVSCSNNKQDPYQYIQRIPHEAVQEIHLAGHSIVYQDNT
                     NFLRIDTHNNYVCPEVWDLYRYAIHYTGLVPTLLEWDTEIPSLDTLLNEARKSACYAY
                     N"
     gene            22593..22859
                     /locus_tag="VAH76_000495"
     CDS             22593..22859
                     /locus_tag="VAH76_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385147.1"
                     /translation="MSQNWPTRDKDLQTARVIMEEYANERDSDSLGLFEIVVDQNEKR
                     MNFRLSGWVVILAKHFTSLYGVSQGDYVTRQVISRCITQGQTLH"
     gene            complement(23103..23855)
                     /locus_tag="VAH76_000496"
     CDS             complement(23103..23855)
                     /locus_tag="VAH76_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO1385148.1"
                     /translation="MKFIQGLLLSFFFLVSPLHAQNEPIPLIIGVGGFYPPFIMQGTN
                     NELFGYDIATMTELCKIMKRQCNFRVMRFRQLIPALINKQIDAAISAMSITSERSNLV
                     NFSMPYLLSYSRFLTNEVHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
                     EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLIGYGLGIAVNRE
                     NSVLLKSLNDALVEYQRSDKYKENYNKYMMNF"
     gene            23993..26734
                     /gene="glnE"
                     /locus_tag="VAH76_000497"
     CDS             23993..26734
                     /gene="glnE"
                     /locus_tag="VAH76_000497"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEO1385149.1"
                     /translation="MSPQSDIETLISTKSWLIEKYVAPLHHPLRESAEKLILVSDYAC
                     RQIRLLVSLLESDSCCSLWSRESYFYAIQQISLELSQAQYMQELRRFRHTHFLRLLLL
                     EIADLASTQEVMQSWSDLADSIILHTLKYIGFTLLSRYGVPRNSEGKEIQLYALAMGK
                     LGGRELNYSSDIDLIFAFNEVGSTDGEEQITNQHYFSKMVQTFVHLLQSVTPDGFVFR
                     VDLRLRPNGDSGPLVSSFAAMETYYQEQGRDWERYAMVKARVIAESLNEPFPWFERLI
                     IPFVYRRYVDFGVIESLRGMKAMIEREVQLNPLLNDIKRGRGGIREIEFIIQNIQLIR
                     GGRLPQLQVQNAMLALAVIKKEKLLPRCDALKQAYLFLRKLENILQSLNDQQTHSLPE
                     DQLKKVQIVYAMGFTDWEQFFNKLQQYQRIVSNFFHSILGKVDDYEDEKRLLNNQLFS
                     LWQGHVESNMAVNLLISLGYENAQHCYQMLHAFRHGPRCRRLSQGARIRLDRFMVLLL
                     TELTYCPKTDEVLLQVIHLLENIVGRSAYLALLTENPHALRELLFWFVNSPFISSLFV
                     SQPFLLEILLEQEKDWHPDSLSQMEQTIVEKLEHTNDVEVHEEILRQFKLTNWMVAAR
                     AELYGLCDAVRIGKFLSDVAQVIVSQVLIIASKQLCGRHPEMAQIQSRFAIIAYGKLG
                     SREMSYASDLDLVFLHSAKPSEEALVTRLTQKILHMLTTRTQMGVLYHVDTRLRPSGA
                     AGLLVSHVDAFVEYQKSQAWTWEHQALLKARILSGNSKIKNQFSQLKKSVLLLERDSK
                     LLQRDVLTMRSKIDQFQESDEVKLARGGLLDLEFLVQFLILNLRDPKFSSYTNTLSQL
                     KHLFLSNVLTKSQFTTLKKAYQYFHYLMHQKIVQPDVINDDEMQNAVSSVCKDLYRY"
     gene            27002..28213
                     /locus_tag="VAH76_000498"
     CDS             27002..28213
                     /locus_tag="VAH76_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1385150.1"
                     /translation="MYANYYDMYQKTGIFSDEFFGQFLRTLGKCYVRCADVGECYATL
                     SKITDKDGESWYKAWYEMANTLKTKANISWEANHFITAAYAYLRAAEYYRASEFFLRA
                     NPDDPRVIPCFDQLQFCFERAMHRLHPGSQKIWIPYDDQTLSGYLFFSDQSPKATLIV
                     PGGYDSTFEENYALVPAALLHGYHILIFDGPGQGHVLRRQKLYMRADFEHVVSQVVDW
                     LDEQSELGTSSYVLIGRSFGGYLAPRAACSEKRLAALICDPGQMDIAGSLNKMLPPNL
                     MMLLKEGKADELNACFYQLFSENPMKEFYFKSRMYTHGLATPFDYLQALSAYTFVDRV
                     QEITCPTLVCDNPSDRISNRGNTLYEALRCEKTYVMFDAKLGAGMHCEADANGQFQQV
                     IFDWLDERFTQ"
     gene            28281..28853
                     /locus_tag="VAH76_000499"
     CDS             28281..28853
                     /locus_tag="VAH76_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEO1385151.1"
                     /translation="MRIGIAILVVLVCCTLQGCKKEKSPYQTTSYVESQLFIVSSPDG
                     GYIKEWYADEGQLLHKEDKIVTLDGVNSIKAPADSVMTERYYLKDEYVPPNFPIASLS
                     LPSQMKILFYVPESHLEKIKLGKKIRILLNEKKYSGKISFISNQAEYTPDAIFSEKNR
                     YKLVYKVKADLSQGLRDLLKIGQPVDVNYE"
     gene            28846..29772
                     /locus_tag="VAH76_000500"
     CDS             28846..29772
                     /locus_tag="VAH76_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385152.1"
                     /translation="MSKWSSKQIIIDVSGLAKSFNGINAVVNLNLQIEEGTIFGFLGP
                     NGSGKTTSLRLLSGLIIPDKGQGRCLNLDLMTQTKLIQAQIGYMPQNFCLYQNLTVYE
                     NLDFIARIYQLKNRKERLKEIIELLALSDNQKRITATLSGGWKQRVALAAALLHKPRI
                     LLLDEPTSGIDPQSRLLIWAHIQSIVSKGVTVLLSTQHMDEAERCHQLVYMSAGEIIA
                     RGSVIEIIQSTGLHSWVVKGESFSRLKDILVNNPAIQMIEKGNEMRISSIVDDIHQVL
                     DPFVLNNYEIKKTDTTLEDAFIYKIMKEGNPK"
     gene            29787..30905
                     /locus_tag="VAH76_000501"
     CDS             29787..30905
                     /locus_tag="VAH76_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385153.1"
                     /translation="MQRLWAIIRKEFLLMKRDPATIVIMAILPLILVCIAGYAINTYP
                     QKVPTVLINLDNTDVTRELVREIQNTGYFSFVASTNDSESAHHLLKTGKAILVLTIPV
                     NFTKALRRNQNPALLLEDGSIDAIASGRAIIALAGLKSSFIEKMGQKGINIPLLRPDF
                     QIINHRLYDPDRVTQFYVVPGMIGLVLMLTMLLITVVVAFRDVQGGTIEYLLASPTRP
                     SEVLLGEIFSYILIGYLQLSLGLILSYYLFHVPFIGAPLLLYLCTLPYIVAELSLGLT
                     IATFCTSQFEAVQVINVFIAFSILLTGFTFPIFGMPEWAQYLGLFLPLTHFFKIMFGI
                     MLKGNNFGEIWANLWPLLAYCLTMISLATWRFKRHFVR"
     gene            complement(30975..32294)
                     /gene="rimO"
                     /locus_tag="VAH76_000502"
     CDS             complement(30975..32294)
                     /gene="rimO"
                     /locus_tag="VAH76_000502"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEO1385154.1"
                     /translation="MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVV
                     INTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYE
                     EVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
                     QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCE
                     QLGELGIWVRLHYVYPYPHVDDIVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSE
                     NTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVE
                     GAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIAR
                     SKSDAPEIDGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA"
     gene            complement(32384..34144)
                     /gene="dsbD"
                     /locus_tag="VAH76_000503"
     CDS             complement(32384..34144)
                     /gene="dsbD"
                     /locus_tag="VAH76_000503"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEO1385155.1"
                     /translation="MKKWLLFSLLFLTSLCVHAEPLPASEVFKVSVKKIDPNTFAIQW
                     NILPKYFLYSDRIQLNSDNDDIAQLGTLRFPTPLTKTDKQGRTFKVYRNQLTLPVGVL
                     GITPGETIVNLRFQGCADDGFCYPPEVKQIKLAIDDKLALSQVDLETFHAPEAKPLEK
                     PEQDIADIFANHNWIMILLIFYGFGLLLSFTPCILPMVPVLSGIIVGHGRTATTKKAF
                     FLSLSYVLSMSVTYAIVGAVVALLGANLQISMQSPWAISLFSLIFVLLALSMFGFYEF
                     KLPDAWQSKIVGSSREQRGGHYLGAAIMGCLSTLILSPCVTAPLIGVLTYIAQTGNVL
                     LGSVTLFTLSLGMGTPLLLIGTSAGKWLPETGSWMNAVKAFFGILLLAVAIYLMARIL
                     PAGLVMGLWACLLIFSGIYSGALTKSNTNQEKLCQGIGIILLTYGLLILIGASMGSSN
                     PLQPLANLQAAPTVSNAFESAKAQSVKSVELAIKQAFGKPVMLDFYADWCASCKVMEN
                     TTFKDPRVQKALSHFIVIKVDVTANNKNDRALMQHFRVVAPPTFIFFNADGVQLNNLK
                     RVGELNADEFMQTIKSIDEN"
     gene            34330..34620
                     /gene="groES"
                     /locus_tag="VAH76_000504"
     CDS             34330..34620
                     /gene="groES"
                     /locus_tag="VAH76_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO1385156.1"
                     /translation="MKIRPLHDRVVVRRMEEERTTAGGIVIPDSATEKPMRGEIIAVG
                     AGKVLENGDVRALAVKVGDVVLFGKYSGTEVKVDGKELVVMREDDIMGVIEK"
     gene            34645..36294
                     /gene="groL"
                     /locus_tag="VAH76_000505"
     CDS             34645..36294
                     /gene="groL"
                     /locus_tag="VAH76_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO1385157.1"
                     /translation="MMAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSY
                     GAPTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVE
                     GHKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGA
                     IIAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHP
                     FILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKICAV
                     KAPGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIID
                     GEGKATEINARIAQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESP
                     MRQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAA
                     SVASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM"
     gene            36417..36857
                     /locus_tag="VAH76_000506"
     CDS             36417..36857
                     /locus_tag="VAH76_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEO1385158.1"
                     /translation="MSKVIKKLEVALADTYALYLKTQNYHWHVTGPQFKSLHELFEMQ
                     YKELAEAVDQIAERIRIMGHKAPATFAEFNQLKTIKDGDSGLSANEMVSELAKDNMMI
                     VKDLNQAIKIAQENEDEGTVTILSDRVAAHEKAHWMLSASKEKK"
     gene            37114..39231
                     /locus_tag="VAH76_000507"
     CDS             37114..39231
                     /locus_tag="VAH76_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEO1385159.1"
                     /translation="MFRFLFPVSLFISSILLFSIQPMVAKAILPIYGGTPGVWTVCVL
                     FFQFILLIAYGYVWLLSQIKKPAVWRLIHMVLVVLSFTAFPLLFHPAARDGQPEWVIL
                     HNLLIQLGLPLLVIGASAPLLQFAYSQTKSKGASDPYYLYISSNLGSLGALLIYPWVI
                     ERFIGLTHQFYFWNFGFAIYSLLLVTVLFFTKYQPLMLTEKKEVDFLPWREIAYWIFL
                     SFVPCSLMLGVTLYITTDVAATPLFWVLPLALYLLSFVFTFTATPFISQKWISRNCLF
                     FLVFTILGFIFGVSQIRVWQLVLFNLLSFFILALLCHGQLFARRPKPYKLTLFYFCLS
                     IGGVLAGIFNGVLAPNWFNYVYEYPLAILLSLFVLPLPKTNRGWWVPLVVLGLLSLHY
                     FIPTVPWFKSTTTFHILAILALGIIVIWHRNKINLVLSMLILFVFLYFPPLQDKQILL
                     KERNFYGVKQVFKKDDVHALISQSTLHGMQVMNGEKASYGRSSYYGAIAPIVDILKKE
                     FHPLSATIMGLGAGTLLCQFRATDSIKVIEIDQQVIDLAKNNKLFTYIRDCSPSAEII
                     KQDGRLALVNMPDASQDLLILDAFNSDAIPVHLMTLEAFTLYKKKIVEDGVILVNLSN
                     RHLQMLPVINAIGRALNMITLFLAHKGDKKLGQFNSEWAMLTSNQSLAFQVMKGTNWK
                     FVADDDQFLWTDDYSNIIPLLKW"
     gene            complement(39383..>39687)
                     /locus_tag="VAH76_000508"
     CDS             complement(39383..>39687)
                     /locus_tag="VAH76_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEO1385160.1"
                     /translation="LGMGLRRNQSSGKTRVNMLFFLAVLLIMIAWWFGLIIESLNKHR
                     SYQANTIKNKRVRSFIHLARMAYRHEPELLNWDLFQNIMSDLKQQYHSFIECGALS"
BASE COUNT        11788 a         7049 c         8260 g        12590 t
ORIGIN      
        1 gttttaatgt tttactgcta tcaaagtaaa aataacgcca gtaataacat taatcatttt
       61 tatttaaaaa gcgcaataaa ttcatgtgcc aataagacta cagaaataat aatcactgca
      121 aactgtgata ttttacctcc tggaacccaa aagggtgatt tgtaacgctt tctgccaaaa
      181 taacacatta gggctggcaa taacagtagt aatatgatgc aaaatatacc tgcgtagcgc
      241 agagcataaa tataagcccc tggataataa ataacgatta gtaaaggcgg caaaaaagtc
      301 aaaacaaata aaccaattcc atggcggcct cgctctcgca attttaagcc atcagcaaga
      361 aaactaaata aacaaagtga tacacctaag aaggcagtta acatgcaaat ggaggtaaaa
      421 aaattaaaga gggcgctgat tgtggtattt tcaactttat cagttaacaa actggctaac
      481 gcgctggttg tacgcggatt ttgcattaga ctttcaagtc cgctagtgcc ttctgaaggg
      541 agtgaaccaa taattacggc atcccaggct atgtaacata tcaaaggtat taatgaacca
      601 ataaaaataa ctttttttaa tgtcttaatg tcatcatcaa aataatctct cagatttggc
      661 acgataatag caaaaccaaa ggaagtaatt aaaatcataa ttgcgccacc aatttgtcga
      721 taatcaccat gttgaaaata gtgaaattcg atgtgtggag caatcaggat aacaagtatg
      781 cagtaaacag ctaatttacc gaacatgaga gcgcggttag caacatcgac ggaatggatg
      841 ccaccatata ccaccaggcc aaaaattaaa gtaaataacg aacttgcctg ccaatcactg
      901 agatgccaat tgactttgga caatagactg ttcaatacat cagcgccgcc ggaaatgtaa
      961 gcggaaagca aggtatacaa taagaaaagg taactaatcc aggcagtaag taacccataa
     1021 tcgcccagag ttgccttggc catggaaacc atgtgttttc ctctggacaa atagagattt
     1081 gcttccagga tgaaaaaggc accaaaggtc ataaaggccc agcagataag aagaaacaga
     1141 gaagattgcc aaaaaccggt tgctgcatta gctactggta gagccaacat ccctccacca
     1201 atggaggtac caactatcaa taagatgccg cctataaatc ttgaacgcat taataaacct
     1261 ttattttaca taagccattt gtggttgtgg gggttgagca gggcttgtaa accactggat
     1321 ctcgattctt ctgttctgag cgcttccatg aataatagca ttgtcagaaa tcgcattctt
     1381 atctccatag ccttctgctt ttagtctttt ggctgcaata ccattagccc agagaaaagt
     1441 catcattgtt tcagcttgag cttgagaaag ttttctctta tggctgcgtg atccgacatt
     1501 atcagtaaat ccggcaacat aaatcgtact ttgaggataa aaatttaaca gtctgatgac
     1561 attgtttagg ccaggatagc atatttcatt aaggcgaggg ctgctaaaca tgaaatattt
     1621 gtctgtagga ataattaatg ttctggtatc tccatattca acatactgaa tatcctgctt
     1681 ttgcagatct ctgatgattt tgcgtttgct gtcacggtag atactcgcaa ccagacctac
     1741 ggtaccacca gccgctgccc caataagagt tcctgaagca gtaccactgg ctattacccc
     1801 agctacagca ccaatccctg tatctactcc tactctctta acagctcgtc tatcgggttg
     1861 aaagttgtta taagggggat gaaagcagcc cgcaagaaag caaatcatta atccataacc
     1921 aaggattcgt gttttggcta atttgcgtga catatccact cctttaatca atgatttaat
     1981 cattattaag catctcatta aatttcgcaa tctaacactt tatattttta caggacgtat
     2041 aaaaaaataa ttttcttcgt ctataattga aatagtcaag gaagaaacag agtgatgcac
     2101 tgtgaaagga aattccgtgt gctcactcgc ccaaatgcaa ggagcggtat gcctggctca
     2161 ttacccgggt taccgaaaaa gctatgaaaa ttccagccca actcaaaaat ataggcggta
     2221 atatcccaga aaatgatatt gctttgcaag tttcttattt tattaaatct caaaatgacg
     2281 aattgcttcc ccaaagtgac gcttatattg ctccagcttc aggcaagatt gatgtcagtt
     2341 ttggaaataa tctcacgcga aataaagatg ttttctttgc tgtaatcagt actgcagaca
     2401 agaaaataat tttgctgaat cttaaggatg ataattgggt ttgatatggg catccagaat
     2461 caggtaatgc aactctatgt ttatatgaga tacacccttt aggggtaaag tgtctgtgta
     2521 aatttggctc cattccctaa cggccgtagc tctgattatt aatcagcatc aacttgctct
     2581 atgtccccga tttaaaacta ttttaaaata ataaattatg gcattatgac tttctattta
     2641 tgctcaattg ataagaacag aacttacgaa aaaaaccaag gtccaggcaa aaaatgtttg
     2701 taatgaggga gtttagatag actaaatgac cgcattgaaa acatttttaa caaagagatc
     2761 ggagtttttc ataagtcctg aagaaaatat aatgcgaaca aagggttagt acgccaaaga
     2821 attcatggag aactatatga gtaaaaaagc gatagcaata agtatcgtca tcgcactttt
     2881 tttcatcaca gcaatttggt ttgcgtacag ctttgttaaa caggaagaaa atcaaaattg
     2941 gcttactctt tacggaaata ttgatatacg tgacgtttca ttatcctttc gtgtctcggg
     3001 gcgtatcctg tctatggacg ttgatgaagg tgctcatgtt aagaaggggc aagtactcgc
     3061 caggcttgat aaagatactt ttatagccaa tctggaaatg gcgaaagccc aattggagca
     3121 agccattgcc aatcaaaaaa atgcaagcag aacttataaa agacgtgagc aattagttga
     3181 tgacggggct gtttcaaaag cgctttttga tgatgccgtt gcgatgaaag actcgagtag
     3241 tgccgaagtt tctgtggcta aagcgaatct tgagaaagct caaatatcat tgaacgatac
     3301 ggaaattcat gccccgactc acgggacgat actgaccaga attcaggagc caggggctat
     3361 tgttaatgtc gcggaaccag tttataccct tgctattgat aatccggtat gggtaaggac
     3421 ttatattgat gaaccttttt taggtagaat ttatcctgga caggacgctt tggtttacac
     3481 ggattccaat ccggaaaagc cctacaaagg tcatattggc tttatttctc cgcaggcaga
     3541 attcacacca aaaattgttg aaaccactca attgagaacg gatcttgtct atcgtataag
     3601 agtcgttatt gatcaaccgg ataatggatt aaggcaagga atgcctgtta ctgtgaaaat
     3661 ccatttaaaa gcgaattcat agtaatgaat agttcaaaac cactagtcag tattaatgga
     3721 gtcagcaaat gttttcctgg aaacactgat ccggcattag ataatgtttc tgcaacaatt
     3781 tttgaagggc aaatcacggg tttggttgga ccggatgggg ctgggaaatc aactcttatg
     3841 cgtcttatat gcagcctgat gttgcctacg caaggaacaa ttacagtaga cagtctcaat
     3901 acccggactg actcggaaaa aattcatgcg attactggct atatgccaca aaaatttggt
     3961 ttgtatgaag atttgaccat tatagaaaac ttgaggctct atgctgaact tagaaatctg
     4021 tatggcgaga aacgtgaaga gcaatttaga acattactta aatttacagc actggagcct
     4081 tttcagaaac gtctggctgg taatctctca gggggaatga agcaaaaatt ggggctggct
     4141 tgtgccttaa tgggtgaacc taagctgttg ttacttgatg agccgagtgt tggcgttgac
     4201 ccaatatcca gaagggagtt gtggactatg gtccaggggc tgcttggaag gggtatgggc
     4261 gttgtttggt caactgctta tcttgatgaa gctgaaaagt gtgaccaaat ccttttactc
     4321 aatgccggca aaccaatata ccatggaaaa ccgggagatt ttttgcaaaa aaccaaagac
     4381 cggacttttc aaatttgtgg tgtttccctg ctaaatcgtc gccaagctct gatgaatgcc
     4441 cttaataccc ctgaagtcat tgatggtgtc atccagggta agaatattcg tattgtcatt
     4501 agtgataaaa aaataaagcc aagtttggat ggtataactc gtgaacccgg agttcatttt
     4561 caagaagttg aatcgcgttt tgaagacgct tttattgaca gtttgaaaac taaaataagt
     4621 ggtcgctctt tgttagccga gcatattgct gaaaagcctc atagtgacaa gcctatcatc
     4681 gaggcccgaa agttaacgaa acgttttggt agttttactg ccgtttcgag taatgaattt
     4741 acaatcaaac gcggtgaaat ctttggttta ttaggcccta atggagcagg taaatcaacc
     4801 acatttaaaa tgatgtgtgg tttattgcag cccacacaag gacaagcatt tgtaatggga
     4861 ctcgatctaa agacttcatc ctctgaagca agaagccgta ttgggtatat ggctcagaaa
     4921 ttttcactat acagtcattt ggatgccttg cagaacatgc gctttttttc tggtctctat
     4981 gggttatccg gagaaaggca ggaagcacag atcaacagca tgattgaaat ttttgatctt
     5041 aaaaagcact taaagcaaaa ttcgggtgat cttccattag gatttaaaca acgtctcgct
     5101 ctggcttgtg ccgtaatgca tgagcctgat gtgttatttc ttgatgagcc gacttcgggg
     5161 gttgatccat tgacccgacg tgagttttgg acacatatta atggtatggt gaataaaggt
     5221 gtgaccgtga tggtaaccac tcattttatg gatgaagcgg aatattgcga ccgtatcgct
     5281 cttatttaca gagggaaaaa cattgccaca ggaacgcctg atgacttaaa ggataaagta
     5341 cgtaactcca cattacccaa tcccacttta gaagacgcgt ttatcgagtt aatccgattg
     5401 gatgagttgc gtgagccggt acattaacac agggagaata gtggttgaat attcgctatc
     5461 taaaggcatt ggtcattaag gaaactctgc aaattatccg tgatcccagt actatattaa
     5521 ttgcctttgt attgccttta attttgcttt ttattttcgg ttatggtgtt aatctggaca
     5581 ctgatcaagt caagtttggc ctggctttag agagttatga ttcgtcgact gtcagtcttt
     5641 ctcaatcttt tagcagttcc cgatttttgg atgtatctat ttcaacaaac agacaagcgt
     5701 ttcgttatgg gttggttaat ggaacaattc gtggttttat tgatattcct caacgtttca
     5761 ctgctgatag tctgagtgga aatcccaagc cagctctgca aaacatagtt gatggttctt
     5821 atccgcaagt tgccggtttt gcagaaaatt atgcgcttgg ggctttgcaa acctggatta
     5881 gctcccaagc ttatgagcat gggcaaatga cacttaagtc cggcattaac cttgagccca
     5941 gatactggta caaccaggag ctaaaaagca gaaattttct tattcctggc tcaatagtta
     6001 ttgttatgac tttgattgga acattactca cctcgctggt gatcgctcgt gagtgggaga
     6061 gaggtacgat ggaggcgatg atggcaacgc caatttctat tgttgacatc atcttgggaa
     6121 aattaatacc ctattttgtg ctagggatgg gttccatgtt tttatgcact attattgcaa
     6181 catggtatta tggagtacct ttccaaggta ctcttttggc actgacatta gtttcagccg
     6241 tctttctggt tgctgcttta gggcaggggt tattaatatc tactcttgca agagaccagt
     6301 ttgtggcttc gcaattggct ttaatgtctg cgtttttgcc ttcatttatt ctttccgggt
     6361 ttatttttga aatcaattcg atgccaaagt ggatacaatt actcacgcat ctgtttgctg
     6421 cacgttattt tgtgacaagt ctgcaaacct tgtttttaac gggtaacatt tggtctcttt
     6481 tgattcgttg cctttttgcg attgcactga ttggatttgc attctttttt attaccgcgc
     6541 ataaaaccag aaaaaggctt gattaatggc aaaattatct ccagcaacaa gaatttttgc
     6601 tcttgtcatt aaagaaatat tgactttatt caaagatccc aagggacgaa ttgtattgat
     6661 agctcctcca ctcctacagt tattgatttt ttcctttgca gctactatgg aaattaaaaa
     6721 tatttctata ggcattttga accaggattc cggaaagcat ggttatgaag tgattcaacg
     6781 ctttataggc tcaccaactt tttcaaaagt tttttttatt caaaagcaat ctgatatcaa
     6841 acctatcatt gataaccagg aagcaatggt tgtaatgacc atcccacaag atttttctcg
     6901 taaaatcgaa tcgaattcac aagccaatat tcagttaatt ttggatggtc gacgttctaa
     6961 tgcaactcaa attgtaaatg gttatatcag tagtattatt gaaaactata atcaggaaat
     7021 tcaaaaaacg catggtgcaa tcatgatgcc tcccgtgatg gtcactcgaa gttggtttaa
     7081 cgaaaatttg ctttatcttt ggtttaccgt cccttcattg atttgcatct tatccatgct
     7141 gatttctctt gtggttactg ctttatcggt agccagagaa agagagctgg gaacctttga
     7201 tcaattatta gtatcacctc ttactcccta tgaaattctt ataggcaaga ctattcccgc
     7261 cattatcatt ggtttggcgg aaggtctctt gatgtgggtt gctgcggttt ggctatttga
     7321 tgttcctttt tatggttctc cgttactgat ggtttatatt ttgtttattt ttattatgtc
     7381 aacggtaggt gttggcttat ttatttcttc tatgtgtaaa acccagcagc aagctatttt
     7441 gggtgtattt atctacatgg tgcctgttgt gacgctttcc ggttacgctt ctccggttga
     7501 aaacatgccg gaatggctcc aggatttaac gtggtttaac ccattaaaat ttactttaat
     7561 agcagtaatt ggcatttttt taaaaggtat gtcggcttta gaggttttta tcacaacatg
     7621 gccacttcta ttggtagggg ctgttacatt atttattgct ggctggtttt ttaagcagcg
     7681 tatggagtaa gaaacagtat ggacagaaaa ttagtgctac tcttcagtat ttcttgttta
     7741 tttggctgta ctgtcggtcc tgattatcaa cgcccggaca ttaaaattca ggagaaatgg
     7801 ccaacaagca aagtagagaa aaaaatttcc agtagtgccc ctgttcattt aacctggtgg
     7861 aaggcatttc atgatccatt gcttgatcga tacattaacg aggcagctac taataacctt
     7921 gatatcaaag cagcagaagc tcgtatccgc caagcccgcg caatgcaaca aatttccctt
     7981 gccaattttt atccgcagat caatggatca gcaagtgcta attccttgca aatcagtaag
     8041 ggtacgttgg aggggatttt tgcaaactct ccaacatata ctctaattga tagaaatcaa
     8101 tggatatata atttgggttt tgatgcacaa tgggagttgg atttatttgg taaaacaaga
     8161 agaaaagttg aagcaagcaa agcaagcctg gaagaggctt atgaatcacg ccgtaccatt
     8221 ttactatcta cgatagccga gatcgcaaga agttatatag aattgagagg cgcgcaacaa
     8281 ttgtatggta ttgcgcaaaa aaatgtggaa ttgcaaagaa aaacgttggc tcttgtccgc
     8341 caacgctata aagcaggtgt tagcagcgaa gtcgatgttt ctttagccga aggccagtta
     8401 aaaaatacag aatctgtttt acccaattat attacccaaa tacaggtaaa tgcttatcaa
     8461 attgccgtta tgctgggtaa agatccgcaa acaattctca aagatattgc aaaaattcaa
     8521 tctttaccca aaccaccgca aaaagtacct atgggattac gttcagatat tctgcgccag
     8581 cgtccagaca taagagcaac agagaggcaa ttagcgtctg ccgtagctga aattggtgtg
     8641 gcagttgctg actttttccc gcaatttaat ctttctgttt ccaatcttgc ttttcagagc
     8701 acttctgttg atacgttatt tcaatccagt agtggatttt ggctcatggg tcctcaagtc
     8761 agtttaccca tatttcatgg tggccagatt agagctaatg ttgagagtaa gaaggcggtt
     8821 gctcagcaag cagcaataca atacaaaaaa ataatcctcc aggcattgca agaggctcaa
     8881 agtgctcttg tccgctatag ccaatcgctc gatacaaacc atagcttgcg cgctgccatt
     8941 attgctaaca agcgagctgt gcaatttgcc aagaaacgat ttcaatacgg tgaggatgat
     9001 tatttatcac tactgcaatc agaggctgct cttctgaatg ccgagactca agaagtcaat
     9061 tcggaggttt cttcattgac tgctttgatt agtctatata aagcgcttgg cggaggttgg
     9121 gaaaactttg aggcttaaag taggcctaaa catctgtgca tgctctaaaa tcaggaactt
     9181 tatcaataaa ttaatgtaat aagttatgga taaggtaggt cattatcttg aatcgttcgg
     9241 gctgaggagg catttatgcc gtctcgaagc cttgtatgga gtttaatgtt tcgtgccaag
     9301 gcttctagac gatgcttact tatctcctaa gcccaaacag cccctagata tcgacagctt
     9361 cttatataat ccttatccat tacgttaaat tagcaaaaat cagacaggta gctcgcaaac
     9421 gtagatctgg ctatagaacg atttacgttt actaccatga aaatatctct tacaaaggtg
     9481 ttagaaacct attcatatca ggctgttggt ttaaacggta ctgataaaca atataaaagg
     9541 ccattacatt ttttagataa tttcgtgttt cttgccaggg aagtgtttca atccagatat
     9601 ctatttcttt gggcgggtga tttttaagcc aatagactac ttgtctggga ccggcgttgt
     9661 aagcggcagc aacaagaacc gggtggttgc tgaagcgctt ggttaagtgc tgtaaataag
     9721 caatccctat attgatattc ttttgagata aaaacaattg cttatgatcc gaatacggta
     9781 ttttgctggt tttggaaaca gcgctggctg tttgtggcat gacttgcatt agccctttcg
     9841 ccccagcaga ggatgttaca tcttctctaa aaccactttc ctgacgaata atagcatata
     9901 caagctcagg cggtatggcg tattttgacg catatacttt tattgtgtct ttgtaggcca
     9961 atggaaatct taatgctaat tgattattga gtgtgtcatt attgcttaag taaactgatt
    10021 ttccgtgcca ttttagttcg gttgctatcc aatagaccag agcacttgct tcttctttgg
    10081 gtaattcact aatgaagtca tttaataacc ttgatgcttg cagtagctgt ttggagttgt
    10141 aaaaattttt aacttgttct atgacaggtt gatagggttt gagaatctct gtgttggttg
    10201 ttggtttttc attgtcaaag ctgggtcttt ttttcaggcg tttgctggct agaaaaccat
    10261 aataatgtct gtttttagct agtggctcat aaattttaat cgcctctgct tttttccctt
    10321 gttcttccag ggagcgagcc aaccaatatt gccaacaagg catctcttta tttggtgagt
    10381 tgttaattaa tttttcaact tgtttccagt ttttattttt cagagcaaag cggatttgcc
    10441 agtcgactaa cacatcggtg tagtattcag gtttgatttt ggcaaaccat tcctgagcat
    10501 cggcatgatt tctcatggcc ttgtataatg ccaaatgtgc aagaaaatct tgtttttgcc
    10561 gctcatttaa catgatgttg gtttttttct cttgccatag tttcactgct ttatccatgt
    10621 taatggatac taatcttttt aatccataaa gatagaaata atcgttcaat ttgccagggt
    10681 tcaacttgga aatattggaa ggattctcat aaacactggt taagtttttt atttctgtat
    10741 cgtgaggtgt tttgtattgt tttaacaggt aacgagcgag tagaacatta cgtttgtcta
    10801 gtgcaagtgc tatgcgttgc gtaatgaatt tctgatcaaa gttatcctgc tttaacagta
    10861 atgcaaatag ggtattgcat gcctggggtc ttgattctcc tgataaccag attggaatag
    10921 agcctgctat agcctccttg tataatccta aattgaattt cgcaatttgc gcaaagcatc
    10981 tgaggtttat gtcgttggta ggttgataat acttattata atcttcccaa tcctttatcc
    11041 tggccagttc atataaccat ttgtcacgca atttattagc aagcggtgtg ttttctttta
    11101 caaattccaa aaattctggc gatgggttaa taggaagctg ttcataccat gcaagatagc
    11161 gattaaacct gtccatgtag gcttgtccag ataaagcaaa caagttgaaa gaacagagaa
    11221 gcccacataa aaaaattaat aattttttca taattcctct taataaggct tgagggcaat
    11281 tatttggcac ttttaaatgc ttgctaattg ttcacgcata tgtttaatgg tttcactata
    11341 attactgcta ttgaatatag cagaacctgc aacaaattgg ttagctccgg catgtgctaa
    11401 tgaagcaata ttctggggtg tgactccacc atcaacacaa agatcgagat ttgggtgctg
    11461 ttgccgaatt ttagagagct tgggtataac ctccgggata agtttttgac cgccaaatcc
    11521 tgggttaact gtcatgacca aaacaaattt tagattatgg gcacaccagg ttaatgtgtc
    11581 gattgacgta gctgggttga gcactaaacc cgcttcacat ccaaggttat tgattagctg
    11641 caaactcctg tctaaatgaa tagtagcatc tggatgaata ctgatacgtt tcgcacctgc
    11701 tttggcaaat gcttcgatta gagtatcaac tggagtggtc atcaaatgga catcaatcgg
    11761 caaatttgga aattttttga ttaaagcgtc acagaaagcg gggccaaagg tcaggttggg
    11821 tacataatgg ttatccatga catcaaaatg aataaaatca gcgccggctt tcataacggc
    11881 atctacttcc tctcctaaac gggtcatgtc cgctgacagt aaagagggta aaatttggaa
    11941 tgtcatgatt tagtaattca acagtttgct tttacatata ataagctgac tttggtaaac
    12001 attccagcat tacgatttgg attcatatcg agttttattg ttttaatcct ggatttgagt
    12061 tggagcaaat caagataata atactccatc ctctctgatt cgttagagag aggatggtat
    12121 agaatgcttg atgtttcgaa ctatggagtt taaagattca attaattgtc taatttcaaa
    12181 taaaatcgtc tgattaaata atcaattgat aatttccctg gcccggtaca taaaagcatt
    12241 aaaatcatga ttccccattg catgtgatcg gtaagaccaa cggcaccatc cggcgtccat
    12301 aggaattgat aagagaaaac ggcaaataag ttaaatagaa aaagtccaaa taaaacgagt
    12361 ctggatccaa gtcctaatgt aatcaataca ggaaagccca gttcacaagc tgttcccaga
    12421 tacgcagcta aagtaggcga catgaccgga accatgtatt cgttttggaa taaagaaaga
    12481 gtagcctgcc aattactgat tttggttaag ccagatagaa aaaaaacctg ggcaagataa
    12541 aggcgggtta aaaggataaa aattggggca aggaaatcaa aaattttatc agccagcaga
    12601 taggtttttg ctataagtgc tttcatatag cctccagtga aaaatgatta atccattgat
    12661 aacgaagcgc cttggttaga gtgtcaccaa ttttttcttc gctaatccat ttacttaatt
    12721 ctgttaccac tttttgccat ggtgtctggt ttccagagga taagagttcc agaaaaatat
    12781 attcctcctc actaattagc ataatacaag gtaaatcatt atgctgataa ataacaactt
    12841 tttcaggaca acctgatatt tgtatttgtt tatcctgctg aaaatagttc atagcccaaa
    12901 tttggtaaat aggatattca gaacagagaa tttttaaatt gggtgtcaga tgaaaacata
    12961 aagactgaaa gctaatctca tctatgctcg aaaaagcctc aaaatcaaaa gggttaacag
    13021 gggaaagaag ctttaaggtg tgccagtgcc actcaatagc gcataactgc tgcaagtaag
    13081 gcagctcttt agccggctca aaattcgcaa taaattgagg aaggctatct ccgtactgat
    13141 taatgtcata ttgagtggat tcgcaattat tgatatagcg atgcaataaa gcgtaccaga
    13201 agttttcccc gaccaaacgc atacagagag gaaaagttga tttcaaatga ttaaataaag
    13261 tagcatggtg gctttccatg tagacactaa taccatcaaa attggatcga gcgagcctgt
    13321 tttttaaggg gcttgatagt gcctcctctt tgaattgtgc tatccattcc ttttgaattg
    13381 tttcaagcag agtattacgc ataatgattc atactccttt ctgccacacg ctttattatg
    13441 gcttttgctt tacacatttc gtcatacagg agtgaaaaat ctggcaagtc attatcccac
    13501 tcgataactg ttggcgtgtt tataaagaga ttttgagcct tttcataaag ttgccaaacg
    13561 tcttggtata ctttatcact atgactatcc agcaaatatc cctcttgttt tgaaaatccc
    13621 ccaagatgca tctgccatac gtttttggta ttgatcgaat gaagatactt atccgggtca
    13681 tcaccatgat tataccaatt cacgtataaa ttattgatat ccaataagat accacaacct
    13741 gtagcagtac ttaactcatt gagaaactcg gcttcagata atgtattttg tcgataagtg
    13801 acgtatcttg atacgttctc gtacattatt ggctggttta ggtattcctg aatgcagttt
    13861 gttttttcaa taatgaatga gagggtttct tcagtatagg gaaacggtat caattcatgg
    13921 gtaaaaaatt gattggcatg ggtccagcat aaatgatctg atattagctt ggagtttaaa
    13981 caatgagcca aattttttat ttgctgcaaa tattgataat caggttcaga ggatgtggcg
    14041 atagataagc caacgctgtg taaactcatt ggataaaact ctgcaatctt gagcaactgt
    14101 ttaaattgag ctcccgattg tttcatgtag ttatcagcca ggacttcaaa ataatcgact
    14161 gtgggctgtt cctttaaaat atcgtcgatg tgttcaagcc gcaatccaat gcctgaacat
    14221 ccagaataag gcgcggcttg atcggtagaa tgatttgaat acattctaat tagcttttaa
    14281 ctgtgccgtt aaccagcttt tcacaagtac ctttgggcag ataaatccag tcagttgttg
    14341 aaccatctgt tttagattga ccagcacagg catgttgagc tgtaccacaa tcattttttc
    14401 cagctttggc cacaccttgg catttttcca tatcatttgg agtatctgca ttagcaggta
    14461 tggtagccat tgaaagtcca gcagccatca cggcagccat tgttatttta gaaatattat
    14521 tcatgatacc tatcctttgt tatatgtaat taatccatat gttatttaat aaaccccttg
    14581 cctaaccggg ctttttcatt gtattcctgc atgcaaacac agctcctcaa aatgtttatt
    14641 tttttgctct aaaaaacaat caggttaggc aagaggtcta ataaaataac gtagttagta
    14701 tagtaccatt tttcgatttg tgacaggaca tcaacttatt tctagaggat ggaaaaatat
    14761 ttaacatggt ttgtttttat tccaaatagg atagaaaaca tttcttagat ctttgcctta
    14821 tacttaataa atcacagcaa aaggaagagg agtattgtat catggcaaag gataccaaaa
    14881 tcattaatct tgctttacaa ggaggaggtg ctcatggtgc tcttgcctgg ggtattattg
    14941 acagacttct tgaagatggt cgtatccatt ttgatgccat ttctgcaacg agtgctggtg
    15001 ctatgaatgc tgccgtactt gcctatggat ttgcaacggg tggaaaggag ggggctcgag
    15061 aagctctgca tcaattttgg aaaatggtga gcgatgccgg gcagatatac aacccactta
    15121 aaaaaactcc gttggaagaa ttattgggca tagggattga aaattcaatg tcctttttta
    15181 tgtttgattt gatgaccaaa attctttctc cctatcaatt taatccctta aattttaacc
    15241 cacttaagga aatattagag caaattgtag attttgaaaa aattaaatcc tgtaaaagca
    15301 ttaaaatatt catctcagct actaatgttc gcacaggtaa aattaatgta tttgataata
    15361 aaaaaatttc tgccgatgcg gttatggcct ccgcttgtct gcctttcatg tttcaggcag
    15421 taaatattgg cgaggattat ttttgggatg gtggttatat ggggaatcca gctattttcc
    15481 ctctaattta taattccgag tgcagggata ttttaattct tcatatcaat cctatttatc
    15541 gagagcatgt cccggatact gcagccgaaa ttctaaatcg agtgaatgag atcagtttta
    15601 attcttcatt aatgcgtgaa atgcgggcga tcgcttttgt tactaaattg atagacagcg
    15661 gttggattaa ggatgaatac aaaaacaacc tcaggaaact ttatttacat gccattcgcg
    15721 cagatgtatc aatggaatcc tcctcagtag ccagtaagct taaccctgag tggtcgttta
    15781 ttagccatct ttatgaagaa ggacgcaaaa agggagaaga atggctgaaa agtaatttta
    15841 aacatcttgg aaaaatgacc agtattgatc tggatgaata tttataagtt tattatcaga
    15901 atgatagttc acaacataat tagaaatttg cagctataat taaaaccaaa gttattagaa
    15961 atacacagat attttaaaaa ctttcttgaa agggattttg atatgcagca agaagatcgg
    16021 gcaccgataa ctcaaaaaaa tcgttcgttt tcaagaaata cagcgaggtg ctttgacacc
    16081 tttatggaca ctgttgactt tcttggtcgg attggatggc ctgttattga tttggctttt
    16141 cgaatatgga tagcccagca attattagtt tctgcaatac ttctaaccaa ccactgggat
    16201 actgctgttt atttggctaa aaatgaatac ccggttccat ggctatcccc tgaatgggaa
    16261 gctttgttag ggatattggc gcagtttgta ggggggattt ccctgctttt gggtttgttt
    16321 acccgatgcg gagcggcagt tattgttgga ttcgctgtca tgactcaagt ctattaccag
    16381 cctatcgatc ttaatttgct ttggattgca ttaatgttgg gttatgtgtt gcgaggcccg
    16441 ggtccacttt ctcttgataa tttgtttgag catgggttta gtcgcagccc tttaccgttt
    16501 gccatgttga ttgtgacatt tgtagatagg acaagagaat ttttttcaag tttgtattta
    16561 ttttctttac gtatgtggtt aatgatttca cttttactca tcaatcaaca catacagctc
    16621 atttcttctg atgcgctgcc gctattgatg ccatggttgc cattaaattc agcctctatc
    16681 gtttttaaca atgtttcact attcatggct atttttttgg gacttggcct agcgactcga
    16741 gttgttgcaa tttttggatt aatagttgtt cttttgaatt ttacgcccgg agtagcaggt
    16801 ttttatttat actggatggt ggtgatggct attttattta tttcgggtcc agggatcttg
    16861 tcattggatc atttgatctt tcatgcattg aagaaacatt atcctcagct ttctggaaaa
    16921 ccggcattta gcctggatgg tttgccacga gtagttatta taggtgcagg ttttgggggg
    16981 attgcttgtg ctaaggcact acgtcatatg ccagtcaaga ttactttgat tgatagacac
    17041 aactatcatc tttttcaacc ttttttatac cagattgcta ctggaagttt atcaccagcc
    17101 gatattgcaa tttcgattcg ctctatattt ttagaacaat ttaatgcgga aatattattg
    17161 ggtaatgtaa cagatattaa taaagaggaa cgtcttgtca tagctgataa tttcacaatt
    17221 ccatatgatt atctggtcat cgctacagga gcaacgcaca gttattttgg aaaggacagt
    17281 tgggccccct atgctcccgg gttaaaaaca attaacgatg gaacttctgt tcgaagccgt
    17341 ataataaagt cgtttgaact ggccgagatc gctgaaagtg aggaagaacg caagcaattt
    17401 ttaaattttg ttattgtagg cgccgggcct actggagtgg aacttgcagg agccatagca
    17461 gaactggctc gatttgggat agtaaaggag tttcgtcatt ttgacccggc atcagcaaat
    17521 attattctgg ttcaggcagc accgcggatt ttgcctacct tttcagaaca aatatcacaa
    17581 aaagcacaac gctatttgga aagcatgggg gttaaagttt tagtgaatag tatggttgag
    17641 caaattgata gcgatggtgt gattattaat aaggaaagaa tttattcaaa atcagttttt
    17701 tgggcagcag gcgttgctgc ttcccctgca tcaaaatggc ttcagctcga agctgatcca
    17761 gcgggtcgcg tgaaagtgaa tgatgatctc accgtagccg gatattctaa tatttttgct
    17821 attggggata cagctgcttc gaatgcctgg aatggcaagc cggttcctgg tattgctcct
    17881 gctgcaaaac aaggcggtgc ttatgtagca aagtcatatc caaaagaatt tataataaca
    17941 attcaagata caaaccattt aaatatattc attatggtag cttggctaca gtgggtcgta
    18001 aagcggctgt tgcagaattt gatcgtttca aaatcagtgg ggaactggca tggtggtttt
    18061 gggggggagt ccatgtcttt ttcctggttg gctctcgaaa tcgtttaagt gtcattttaa
    18121 attggctttg gtcttattac acttttcgcg ccaataattt gctgattacc gatgattcat
    18181 tgaataaaaa aaataaatca gggacaagcg aggtttaggg ctcattgaag ataataagga
    18241 aatacaaagg gagtgattaa tggacaagta tctttcagca aattctctag aaggggttat
    18301 cgataatgaa tttagcatgc cagctccacg ttggttaaat acttacccag ctggcccata
    18361 tcgatttatt aatcgtgaat tttttattat tgcttatgaa actgatccgg atcttttgca
    18421 agctatttta cctcctgata tggaattatt ggagccggta gtcaaatttg aatttatacg
    18481 tatgcctgat tcaacaggat ttggtgatta caccgagtca gggcaagtgg tccctgtgag
    18541 atataaagga gaagagggcg gatttaccat ttcaatgttt cttgattgcc atgcccctat
    18601 tgctggtggc cgagaaatat gggggtttcc aaagaaactg gccaaaccca aattgtttgt
    18661 tgaagaagat acactcattg gcattcttaa gtatggaagt attgatattg ccatcgcaac
    18721 tatgggatat aaacatcgtc cgctggatac ggaaaaggta ttggaatccg ttaaaaagcc
    18781 tgtattttta cttaaaaaca ttcctaatgt agatggaact cctttagtga atcagttgac
    18841 caaaacttat ttgactgaca ttacagtgaa aggagcatgg accggaccag gtagcttgga
    18901 gcttcatcct catgcactgg ctcctatctc taatctttat attaaaaaaa ttgtatccgt
    18961 ttcacatttt attactgatt tgaccttgcc gtatggagag gttgttgccg attatttagc
    19021 ctaaaataaa aagcgactcc taaaaatggc aggaaaatca tatgaataaa cgtccgatac
    19081 aaaacacggc cgtcattgca gctgctttgg gagctgctct tgcttctata tacacctata
    19141 cagattggct ttcatcagat gaaatcacag taaaaagaga gcgttgctat aatgtggcga
    19201 gagcgggcaa aaatgattgc gctacttccc agcattcttg tgctgcgcaa tcaacggccg
    19261 atcgtgatcc tgaagcattt atcatgttac ctaaagggtt atgcgaacgc atagtggggg
    19321 gaaggagtgg atagttactc taagttaccg cttcgcatca ggaaagagat agaagcccaa
    19381 caatacactg gtgaactatt ggttgccggc gtccagttgg gtgtcattgt attattaagc
    19441 attatcaata tattttcttc agctggctat tcgcctggcg cgccggtgca atcatcatct
    19501 ctaggcttat ggctatttgc tattttgata ttactaaggc tgtggtttgt ctatactaat
    19561 caattaaatc caatattctt gggattcttt atagtcgccg aaatggcttt gttattattt
    19621 attatttgga cttattattt gcagttcgaa accacaccta ctattaatct taaaaatccg
    19681 catattaact atgtttatgt tttaattgct ttgcgtgcgc ttcgatttga accattatgg
    19741 gtaattctct ctggcctcac tgcggctctt ggctggggaa ttattgcctg gcaaacttta
    19801 tccagttcgg gtatgaacgt tattacatgg gattatgtga cattcgcgtc aactcggagt
    19861 gtctatctgg gagctgtatt tgatgtcata ttgtccgttc tactggttac taccatcata
    19921 gccttggtat tgacgcgtgc gaaacacact ctgtttcaag cagtagaaca aactagcgct
    19981 gcaaaagatt tatcacgttt ttttgataca agtgtggtcg aaaaaataac aacatctgaa
    20041 aagtcattgc aagctggata tggcgagctt cgtcaggctg caattatgtt cattgatatg
    20101 cgaggattta ccaaagcatc agtgaaactg tccccgagtg aactaatcgg actgctagca
    20161 gaatatcaac gcttactggt tccgattatt caaaagaata atggcagcat tgataaattc
    20221 atgggggatg gtattttggc aagtttcggt gctgtgaccc cttcaaatac ttatgcagca
    20281 gatgcattaa ggactgtgga tgaaattgtc gcatcggtag acgtatggag tgaaaatcgc
    20341 cgtaaaaaag aacagatagt ggtcggtgta ggaattggtc ttgcttcagg tgaagtagta
    20401 gttggtgtca ttggttatga aaatcgtttg gaatatactg taattggcga agctgccaat
    20461 ttagctgcaa agcttgaaaa acataataag gaagaaaaaa cccaggcttt gactacccgg
    20521 gatacttttt tagaagcggt aaaacaaggt tatgaggaca aaataactaa gaaggaattg
    20581 aatgcaagaa atgtaggtgg tgtgggagaa cctttggatt tagtggtatt ggcaaaataa
    20641 cccatattgt tcttcacggt tattcaggac agttgttagc gtgagtcaaa tgataattag
    20701 aatgaataaa agaatcttcc aataattcat ttcttagtaa aaattgtaat gcagtgagca
    20761 aatcaaaatc gggattattt tccagagtat tttcgtatgc gaacccaagt gaggcccctg
    20821 ctttgatgtg gcataaaaag tgaaacaaat cgtgagtaat ccaatgaatc actgtttgat
    20881 ttttttgacg agatatcacc gcataacttg gtatttgttg aaaatcaata gaacctgttt
    20941 ccgtgggatt ttctatcaga tcaaaaatat tttttaagta ataagaggat gagtataaaa
    21001 acacggaagg attaaatagc aaaattaatt tttctactcg atcatttagg tgtttttgca
    21061 gtttctgggg atcaaaaaca agacaatccc gcgagcaata gcttaaatgc tttaaccatt
    21121 ctatttctgc gatatctttc aaataaagaa gatgactaac ggttggatta ttttttaaaa
    21181 acagaggaaa tttttcaccc caatcatcca aacaatgggt tgttggcaaa ttatttttat
    21241 cctggataaa gtgtaaagcc aaaccatccg cacattcttt tccaaccaat ttccaaatgg
    21301 ctggaaaagt catttccagg gaatggcgta gattctgaaa aatggtatta cgataaatgt
    21361 cgaaacggaa ttcattagta atctcgtcag ggcaaaaata ctcaaaaata gcaatatctt
    21421 gttgatttaa tactgtgttc agaaaaaatt cctgcatttg tttaattgta ggcataacag
    21481 gcggacttcc tggcttcatt taataaagta tctaacgagg gaatttctgt atcccattct
    21541 aacaacgtgg gaacaaggcc tgtataatgt attgcatatc gatacaaatc ccatacctcg
    21601 ggacacacat aattattatg tgtgtctatc cgcaagaaat tcgtattgtc ctgatagaca
    21661 atggaatggc ccgccaaatg aatttcttgt actgcttcat gcgggattct ttggatatat
    21721 tgataaggat cttgtttgtt attagaacag gaaacataga tattatttat atccaacaag
    21781 agcctggctt cagtacgctt gattatttct gctaaaaaat cgggctctga atacgatgaa
    21841 gtctgatatt ccaaataaga agaggggttt tctattaata aggttctctt taaataattt
    21901 tgtactttat caatattatt gctaataatg gtcaaacttt cctcattata gggaataggt
    21961 aataaatcgg gtaaataggt gtttccgatg gtactccagg ataaatgatc agagagcaaa
    22021 gctggttgaa agcggtcaat gagagtttta atgcgattta aatgcgctat acagactccc
    22081 tcagcagacc ctaaagataa cccgactcca tgaaagctca gtggataatg ttggcttata
    22141 gaatctagaa gctctagtaa aggcccaccc ttgaccaaga aattttctgt atgtacttca
    22201 agccaatcaa tatcaggctt ttcctctaat atttgggcat aatgtggcgc tcttaggcca
    22261 atacctgtca tagcaccatt tatgatttta tgcatgatat tttttatccg atttgaagtt
    22321 ttgttgagca attaaaaaac tatgtcaaag atttaatgat atattaatgt actcttgaga
    22381 agtttcgaac gcaatggtct tggaaaaagc tattttcaat gggtatttta aaccataaat
    22441 atttttaatt cagtgtaaaa attaaccgca atattgagta tttaatcact ttatgagata
    22501 atatttattt atttcacaac ttgcaatttt gaaaattgcc tccatgttgt agaagtaaca
    22561 acagtcttgc tcatgttttt agggagtgtc ttatgagtca aaattggcct accagagata
    22621 aagatttgca aacggcgcga gtcataatgg aagagtatgc taatgaacgt gatagtgatt
    22681 cattaggtct tttcgagatt gtcgttgatc aaaatgaaaa acgcatgaat ttccgtttat
    22741 ctggttgggt agttattttg gcaaaacatt ttacttcttt atatggagtt agtcagggtg
    22801 attacgttac caggcaggtg attagtcgct gcattactca aggtcaaact ctgcattaaa
    22861 agctagagat gagccattca attggaatgg ccgtattggg tttatattga aagaaaataa
    22921 tccttcgaaa tgctcacata gtcatctatg ctgtgctttt tcatgattat tttcatccaa
    22981 cctggactca gatccggccc ttacaggata gttaaatatc agggcgctga cttattacgc
    23041 cttaaaacgg ttattcagct attgatgagt gattaagagc aatttttgta accatatcac
    23101 tcttaaaaat tcatcatgta tttattgtaa ttttccttgt atttgtcaga acgctggtat
    23161 tctaccaagg catcatttag agatttgagt aatacggagt tttcccgatt gactgcaatt
    23221 cccaaaccat aaccaatcaa catgggactg cctattacca gaagtctccc cgacgaatta
    23281 gcagaccagt ataaagcggc aggattatcc atcagaacaa agtcaatttt atttgcgctc
    23341 aacgcctcaa gcatttcttc agttctgtca aattccacta tgacagggtc tattatcccc
    23401 atccttctga tttcctgact aaagacagta ttttgttcca ctccaattcg tttacctttt
    23461 aagagcatta aattaaacgg ttgatgagta ttgtgtacct catttgtaag aaacctggag
    23521 taactcaata aatagggcat gctaaaatta accaggtttg aacgttctga agtaatagac
    23581 atggcgctga tcgccgcatc aatttgtttg ttaatcagag caggaattaa ttgtctgaat
    23641 ctcatgactc gaaaattaca ttggcgtttc attatcttac acagttctgt catggtggcg
    23701 atatcatagc caaaaagctc attattggtt ccttgcataa taaagggggg gtaaaagcct
    23761 cctacaccta ttattaaagg tataggctcg ttttgagcgt ggagaggtga aactagaaaa
    23821 aagaaagaaa gcaataatcc ctgaataaat ttcattggta tcttcactaa aatattatta
    23881 gagtctttta taagagtata acgtccaata ggagttttaa agtattaaat gtaaaattaa
    23941 ttgattaatc ctatgatatg ttgaggtttt tatattaaga atagtatatt ttatgtctcc
    24001 acaaagcgat attgaaacat tgatttctac aaaatcctgg ttgattgaaa agtacgttgc
    24061 tcctttgcac catccattga gagaaagtgc cgaaaagttg atattggtaa gtgactatgc
    24121 ctgcagacaa attagattat tggtctctct tttagaaagt gattcctgct gttccttatg
    24181 gtctcgcgaa agctattttt atgccattca gcaaatcagc ctggaattat ctcaagccca
    24241 gtatatgcag gaattgcgtc ggtttcgtca tacccatttt ttgcggttgt tattacttga
    24301 aatagccgat ttggcttcta ctcaagaggt tatgcaatcc tggtctgatc tggcagactc
    24361 tatcatcttg catactttaa aatatattgg atttaccttg ttgagtcgtt acggggttcc
    24421 cagaaacagt gagggtaaag agattcaatt gtatgctctc gccatgggga aacttggggg
    24481 aagagaactt aattattcgt ccgatattga tttgattttc gccttcaatg aagttggcag
    24541 cactgatggt gaggagcaga ttaccaatca gcattatttt agcaagatgg tgcaaacatt
    24601 tgttcattta ttacagagtg taacgcctga tggttttgta tttagagtgg atttaagatt
    24661 acgacctaat ggagatagtg gtcctttggt ttccagtttt gcagctatgg aaacctatta
    24721 ccaggaacag gggcgtgatt gggaacgtta tgctatggtt aaagcgcggg ttattgctga
    24781 gtctttgaat gaaccatttc catggtttga gcgtttaatc atcccgtttg tttacaggag
    24841 gtatgtggac tttggagtga tagaatccct gcgagggatg aaggcaatga tcgagcgtga
    24901 ggtgcaactt aaccccttat tgaatgatat taaacgaggc cggggtggta ttagggaaat
    24961 agaatttatt attcagaata ttcaattaat tcgcggaggc agattacctc aattgcaagt
    25021 tcaaaatgcc atgttagcgc tggctgtgat aaaaaaggaa aaattactac ccaggtgtga
    25081 tgctttaaaa caagcgtatc tttttttgag gaaattggaa aatattctac agagtttgaa
    25141 cgatcaacaa acccattctc tacctgaaga ccagttaaaa aaagtgcaga tagtttatgc
    25201 catgggattc actgattggg aacaattttt caataaatta caacaatatc aacgaattgt
    25261 tagtaatttc tttcattcca ttcttggcaa ggttgatgat tatgaagacg aaaagagatt
    25321 gttaaacaat caattattta gcctttggca aggtcatgtt gaaagtaata tggcagtgaa
    25381 tttattgatt agtttagggt atgaaaatgc acaacattgt tatcaaatgc tccatgcttt
    25441 tcgacatgga ccacggtgca gaagattatc acagggcgct cgcattcgcc ttgatcgttt
    25501 tatggtttta ttgcttacag aattgactta ctgccctaaa acggatgagg ttttattaca
    25561 agtaattcat ttgcttgaaa atattgtggg gcgaagtgct tacctggctt tactcactga
    25621 aaacccccat gcgctaagag aattattatt ttggtttgtg aatagtcctt ttatttcttc
    25681 cctgtttgtt agccaacctt ttttgttgga aatcttgttg gagcaggaaa aggattggca
    25741 tcctgattca ttatcccaaa tggaacagac aatagttgaa aaattagagc atactaatga
    25801 tgtggaagtg catgaggaaa tcctgcgtca gtttaaatta acgaattgga tggtggctgc
    25861 cagagcggaa ttgtatggat tatgtgacgc tgtgcgcata gggaaatttc tgtctgatgt
    25921 ggcgcaagtc attgtcagtc aagttttaat tattgcaagc aaacagttat gtggccgtca
    25981 ccctgaaatg gctcaaattc aatcacgatt tgctattatt gcttatggaa aactgggcag
    26041 tcgagagatg agttatgctt ctgatctcga tttggttttt ttacattctg ccaaaccatc
    26101 tgaagaagct ttagtgaccc gattaacaca aaaaatatta catatgctga ctactcgtac
    26161 tcaaatgggg gttttgtatc acgttgatac tcgtttaaga ccctccggtg ctgcaggctt
    26221 gttagtcagt catgtggatg cttttgttga gtatcaaaaa tcacaagctt ggacatggga
    26281 acatcaagct ttgttaaagg cgaggatatt atcgggaaat tcaaaaatca aaaatcagtt
    26341 ttcccaacta aaaaaatcag tcctgttgct agagagagat agtaaattat tgcaaaggga
    26401 cgtattaacc atgcgatcta aaatcgatca atttcaagag tcagatgaag taaagctagc
    26461 tcgaggcgga ttattggatt tggaattttt ggttcaattt cttatattga atcttaggga
    26521 tcccaagttt tcaagttata ccaatacact aagccaactt aagcatttgt ttttatcgaa
    26581 tgtattaacg aaatctcaat tcacaacttt aaagaaagct tatcagtatt ttcattattt
    26641 gatgcatcag aaaattgtac agccagatgt tattaatgat gacgaaatgc aaaacgcagt
    26701 gtcttcagtt tgcaaagatc tttatcggta ttagattgtg tttagacagc actcccctga
    26761 aaatgaatcc tacagacttg caacccccca atctcgtcgt taaaaaatgt ttttaattcg
    26821 gtcatttagt ttatctaaac tccctcatta aaaacatttt ttgccttgac cttggggttt
    26881 tcgtaagtct gtcctaatat catttaggat aattcatagg cctgcgcgta atatactctg
    26941 gcacatggca atccatttta ctttcagtta ctctgtaatt tttagggaat tttttactgt
    27001 tatgtatgct aattattatg atatgtatca aaaaacaggg attttcagtg atgagttttt
    27061 tgggcagttt ttaagaactc ttggtaagtg ttatgtgcgg tgtgctgatg ttggtgaatg
    27121 ttatgcaacc ctgtccaaaa tcactgataa agacggggaa agttggtata aggcatggta
    27181 tgagatggca aacacactta aaaccaaagc caatatcagt tgggaagcca atcattttat
    27241 aacagcagca tacgcttatc tgcgtgctgc agaatattac cgggcaagcg agtttttttt
    27301 acgtgccaat cctgatgatc cgcgtgtgat tccttgtttt gatcaattgc aattttgttt
    27361 tgaacgagca atgcatcgat tgcatcctgg atctcaaaaa atatggatcc cctatgatga
    27421 tcaaacatta agcggttatt tatttttcag cgatcaatct cccaaagcaa ctttgatagt
    27481 acctggagga tatgacagta cctttgaaga aaattatgct ctcgttcctg ctgcgctttt
    27541 acatgggtat catattttga tttttgatgg cccgggacaa gggcatgttt tgcgtcgaca
    27601 aaagctgtat atgagagctg atttcgaaca tgttgtctct caggttgtgg attggttaga
    27661 tgaacaaagt gaactgggga cttccagtta tgtgctcatt ggcagaagtt ttggcggtta
    27721 tcttgcacca agggcggcct gcagtgaaaa acgattagct gcattaattt gtgatccagg
    27781 acagatggat attgcaggct cactcaataa aatgctacct ccaaacctta tgatgttatt
    27841 aaaagaagga aaagctgatg aacttaatgc ctgtttttat caattgttct cagaaaatcc
    27901 aatgaaagaa ttttatttca aatcaagaat gtatacacac ggtttggcta ctccttttga
    27961 ctatttgcag gcattatctg catacacctt tgttgatagg gtgcaagaga tcacttgtcc
    28021 cacactggtc tgcgataatc caagcgacag gatatccaat cgtgggaata cgctatatga
    28081 agctttgcgt tgtgaaaaaa cctatgtgat gtttgatgcc aaactggggg caggtatgca
    28141 ttgtgaagca gatgctaatg gtcagtttca gcaagttatt tttgattggc tggatgagag
    28201 atttacccaa tagtggatgt cccaatcgtt attgctttgc tgataggata actaatactg
    28261 atgacaaaag gataagaggt atgagaatag ggattgcaat cctggttgta ttagtttgtt
    28321 gcacattgca aggttgtaaa aaagaaaaat caccatatca aacgacaagc tatgttgaat
    28381 ctcagttatt tattgtgtca agcccggatg gtggatacat aaaagaatgg tatgctgatg
    28441 aaggtcaatt actgcataaa gaggataaaa ttgtaaccct tgatggggta aattcaatca
    28501 aagcgcccgc agactctgtt atgactgaac gttattattt aaaggatgag tacgtacctc
    28561 ctaatttccc tattgcgagt ctctctctgc cttctcaaat gaaaatcctg ttttatgttc
    28621 ccgaaagcca tttggaaaaa attaaattag gtaaaaaaat tcgaattttg cttaatgaaa
    28681 aaaaatattc aggcaaaata agctttatat ccaatcaggc tgaatatact cctgatgcta
    28741 tttttagtga aaaaaatcgt tataaattag tatacaaagt aaaggcggat ctatctcagg
    28801 gattaaggga tttattaaaa ataggtcagc ctgtggatgt caattatgag taaatggagc
    28861 tcaaagcaaa ttattatcga tgtgtccgga ttagcaaaat catttaatgg tatcaacgca
    28921 gtagtgaact tgaatttgca aatcgaggaa ggaacaattt ttggtttttt agggccaaac
    28981 ggtagtggta aaacgaccag tttacgatta ttaagtggat taataattcc cgataaaggc
    29041 cagggtagat gtctgaatct cgatttaatg acccaaacca agttgataca ggctcaaatt
    29101 ggctatatgc cacaaaattt ttgcctctat caaaatctga ctgtttatga aaatctggat
    29161 tttatcgcaa gaatatacca attaaaaaac aggaaagaga gactgaagga aatcattgaa
    29221 ttactggcct taagtgacaa ccaaaaacgg attactgcta ctttatcagg aggttggaag
    29281 cagcgggtgg ctttagcggc tgctttgttg cataagcctc gtatactttt gttagacgag
    29341 ccaacttcag gcattgatcc tcaatcgcgt ttgttaattt gggctcatat acaaagcata
    29401 gtatccaaag gagttactgt tttactcagc acacagcata tggatgaggc agaaagatgt
    29461 catcaattgg tttatatgtc cgccggagaa atcatagcac gtggctcagt gattgaaatt
    29521 attcaatcga caggtttgca ctcctgggtt gttaaaggtg agagtttctc cagattaaag
    29581 gacatattag taaataaccc cgctattcaa atgattgaaa agggtaatga aatgcgcatc
    29641 agctcaatag ttgatgatat acatcaagta ttggatcctt tcgtgttgaa caattatgaa
    29701 atcaaaaaaa cagatactac tttagaagac gctttcattt ataaaattat gaaagaaggg
    29761 aatccaaagt gaagagtcat ttttcaatgc aacgtttgtg ggctatcatc cgcaaagaat
    29821 tcctattgat gaaacgtgat cctgctacta tcgtgattat ggcaatctta cctttgattt
    29881 tagtttgtat agccgggtat gccataaaca cctatcctca aaaagttcct acggtgttaa
    29941 tcaatttgga taatactgat gtaacacgtg aactggttcg agagatacaa aataccgggt
    30001 atttttcatt tgtggcttcg acgaacgatt cagaatcagc ccatcattta ttgaagacag
    30061 gcaaggctat cctggtattg actattcctg tgaactttac caaagcactt cgtcgaaacc
    30121 aaaatccagc tcttttattg gaagatggca gtatagatgc tattgcttca ggtagagcta
    30181 tcattgcttt ggcgggattg aaatcatcat ttatagagaa aatgggtcaa aagggtatta
    30241 atattccctt gctgaggcct gattttcaaa tcataaacca ccgattatat gatccggatc
    30301 gcgtaactca attttatgtg gttcccggga tgataggctt ggtgctcatg ttaacgatgc
    30361 tgctgataac cgtggttgtg gcatttagag acgttcaggg tggtacgatt gaatatttat
    30421 tagcctcacc gacccgccca tcagaagttt tattaggcga aattttttct tatatcctca
    30481 ttggttattt acaattaagt ttgggtctta tcttgtctta ctacttattc cacgttccgt
    30541 ttattggcgc ccctcttctt ttatatttat gcaccttacc ttatattgtg gcggaattat
    30601 cgttgggttt aactatagcc actttttgta catctcagtt tgaagcagtg caagtgatca
    30661 atgttttcat tgctttttct attctgttaa ctggatttac ttttccgata tttggaatgc
    30721 ctgaatgggc gcaatattta ggattatttt tgccattgac gcattttttt aagattatgt
    30781 ttggcattat gctaaaagga aataattttg gtgaaatttg ggctaattta tggccactac
    30841 ttgcgtattg ccttaccatg atttctttag cgacatggcg atttaaaagg cattttgtac
    30901 gctagataca ataaatattc tctctcttgc caatgaaggt gagcagaaaa agtactgttt
    30961 taatgccatt tctttcatgc aagagagtat tccaaatcac catacaaatc atagtcgtca
    31021 ctgtcggtta tcattgcttc tgcgaaagac ccaacagtaa tcccggatat ttttggcaaa
    31081 taaacaagac catcgatttc aggagcatcg cttttactac gagctatgat ttgatcttcg
    31141 gttatctcat caattaaaac agtttgagta ctgccaattt tattttttaa tttattacgg
    31201 ctaatttctg cttgtacttg cataaaacga tgataacgct cttctttaat atcctccgat
    31261 acaggattat ccaaatcatt tgctttggct ccttcaacag gggagtattt gaaacaaccg
    31321 actctgtcta gttgtgcctc tttaaggaag gctagtaatt cggaaaactc ctcttctgtt
    31381 tcaccaggga aacctacaat gaatgtggag cgtaacgtaa tgtcagggca aatttctctc
    31441 catgaagcaa tccgcaataa ggtattttca ctgctggcgg gtcttttcat tgcttttaat
    31501 atgcgtgaat tggcatgttg caatggtata tccaggtaag gcaaaatcag gccatcgcgc
    31561 attagaggaa caatatcatc aacatgggga tagggataga cataatggag tctcacccaa
    31621 attcctaatt cgcctaattg ttcgcataga tcgtaaaaac gggtattgac tgtttttcct
    31681 tgccattcga cctgctgata acgtgtatcc acgccataag cgctggtgtc ttgtgagata
    31741 accagcaatt ctttaactcc ggcttgtttt aatttcttag cttcagtaag aatttgggcc
    31801 attggatagc tttgcaattt tcctctcatc gtagggataa tgcaaaaagt gcatttttgg
    31861 ttgcatcctt ctgatatttt taaataagca taatgccgag gtgtaagttt tattccctgt
    31921 gggggaatca gttgtgtaaa tgggtctgca ggaggcggca agtgttggtg taccgcattg
    31981 actacttctt cgtaggcatg agcaccactg atatgcagga catcaggaca ggcattttta
    32041 attacgtccg ctttagctcc taaacaacct gtaacgatta ctcgcccgtt ttcagccatc
    32101 gcttctttaa ttgtatccag tgattcctgg acagcgctgt caatgaagcc gcacgtatta
    32161 attacgacga ctccagcatc ctcataagtg ggaaccaatt catagccttg tgctttaagc
    32221 tgagtaatga ttcgctctga atcgaccagt gctttggggc atcctaaact tacaaatcct
    32281 actttatgat tcataaactt ctatctgtaa aaaaaatgag ctaattatac tgcttaatcg
    32341 ttcgagtgtc gactgatttc atacaaaagt gcccgtagtt attttagttc tcatcaatgc
    32401 tttttattgt ttgcatgaat tcatcggcgt tcaattcacc aactcttttc aaattattga
    32461 gttgtacgcc atcagcatta aaaaagataa aagtcggggg ggctactaca cggaaatgct
    32521 gcattaatgc tctgtcattt ttattattgg cagttacatc cactttgata acgataaaat
    32581 ggctcaaagc tttttgtaca cgaggatctt tgaacgtggt gttttccatc actttacatg
    32641 atgcacacca gtcagcataa aaatcaagca tcactggttt tccaaacgct tgttttatag
    32701 ccagttcaac acttttaaca gattgggctt tagctgattc aaaagcattg cttacagtag
    32761 gggctgcttg taagttggct aaaggctgca aagggttgga agagcccatg ctagccccaa
    32821 tgagaatcag caatccatag gttaataaaa tgataccaat gccttggcag agcttttcct
    32881 ggtttgtatt tgatttggtt aaggcacctg agtaaatgcc ggaaaaaatc aggagacaag
    32941 cccatagccc catgaccaga cctgctggta aaatgcgtgc catcagatag atggcaacag
    33001 caagaagtaa tatgccaaag aaagctttaa cagcattcat ccagcttcct gtttcgggta
    33061 gccatttacc agcagaagtt cctatcagca gtaatggagt acccatgcct aggcttaatg
    33121 taaatagagt aacactgccg agtaatacgt ttccggtttg tgctatgtag gttaatactc
    33181 caattagtgg tgctgtaacg catggtgaca gaatgagtgt ggacaaacat cccatgatgg
    33241 ctgccccaag atagtgtccg ccacgttgtt cacgacttga acctacaatt ttgctttgcc
    33301 atgcatctgg tagtttgaat tcataaaagc caaacatgga caaggccagc aaaacgaata
    33361 tcagactgaa tagactgata gcccaaggtg actgcatact gatttgtaaa ttggctccaa
    33421 gcaaggcaac gacagcaccc acaatagcat aagtgacaga catgctaaga acgtagctta
    33481 atgacaggaa aaacgctttt ttcgttgttg ccgttctccc atggccaaca ataatacctg
    33541 aaaggacagg aaccataggc agtatacaag gcgtgaagga caggagcagg ccaaacccgt
    33601 aaaatataag cagaatcatt atccaattat gatttgcaaa aatatcagcg atgtcttgtt
    33661 caggtttttc aagaggtttt gcttcaggtg catggaatgt ttccaaatca acttgagaaa
    33721 gagccagttt atcgtcaatt gccagtttga tttgttttac ttcgggggga taacaaaaac
    33781 catcgtcagc acatccttga aaacgcaaat tgactattgt ttctcctggg gtaatgccca
    33841 ggacccccac tggaagggta agctgattgc ggtaaacttt aaaggttcgc ccttgtttat
    33901 ccgttttggt taagggggtg gggaagcgaa gagttccaag ctgtgcaatg tcatcattat
    33961 cggaatttaa ttgaatacgg tcgctatata aaaaatactt gggcaagata ttccattgta
    34021 tggcaaaggt attggggtct atttttttta cactaacctt aaatacttca gaagctggta
    34081 agggctcagc atgaacacaa agggaagtta aaaataatag ggaaaataag agccactttt
    34141 tcattcgata tcttttacag ttactaataa acattattgt agactctgta atcccaaatc
    34201 caaaattttt tttgatttta cccttgaaag tttgtttatt gtccccatat cgctgtcatc
    34261 ggtattgttt gccctaagga aagcaatact taaatgattt aaagtttaat ttaatcagga
    34321 gagaaaagta tgaaaattcg tcctttacac gatcgcgttg ttgttcgtcg tatggaagaa
    34381 gagcgtacca ctgctggtgg tattgttatt ccagatagtg ctaccgagaa acctatgcgt
    34441 ggtgagatca ttgctgtcgg cgctggcaaa gtattggaaa acggtgatgt tcgtgcttta
    34501 gcagttaaag taggtgatgt ggtattgttt ggcaagtact ctggtactga agttaaagtt
    34561 gatggaaaag aattagttgt gatgcgcgaa gacgacatca tgggtgtaat cgagaagtaa
    34621 tctaattgtt taaaggagac aatgataatg gctaaagaat tacgttttgg tgatgacgct
    34681 cgcctacaaa tgcttgctgg tgttaatgca ttagcagatg cggttcaagt tactatgggt
    34741 ccacgtggcc gtaatgttgt attggaaaaa tcttatggcg ctcctactgt aactaaagac
    34801 ggtgtgtctg ttgccaaaga aattgaattt gagcatcgtt tcatgaacat gggcgctcaa
    34861 atggttaaag aagtggcttc taaaacttct gatactgctg gtgatggtac tactactgca
    34921 acagtattgg ctcgttctat tcttgttgaa ggtcacaaag cagttgctgc tggtatgaat
    34981 ccaatggatc tcaaacgcgg tattgataaa gcagtattag cagttaccaa aaaattacaa
    35041 gctatgtcta agccatgcaa agacagcaaa gctattgctc aagttggaac tatttctgct
    35101 aattccgatg aagcgattgg tgctatcatt gctgaagcaa tggaaaaagt tggtaaagag
    35161 ggtgttatta ccgttgaaga tggtaatgga ttggaaaatg agctttctgt tgttgaaggt
    35221 atgcaatttg atcgcggtta catttctcca tacttcatca acaaccagca aaacatgagc
    35281 tgtgaacttg agcatccatt cattttattg gttgacaaaa aagtttccag tattcgtgaa
    35341 atgttgtccg tattggaagg tgttgcaaaa tctggccgtc ctttattgat catcgcagaa
    35401 gatgttgaag gcgaagcttt agctactctg gtagttaaca acatgcgcgg tattgtaaaa
    35461 atatgtgctg tcaaagcgcc tggttttggt gatcgccgca aagcgatgct gcaagacatt
    35521 gctattttga ctaagggtca agtgatttct gaagaaattg gcaagagcct ggaaggtgct
    35581 actctggaag atcttggtag tgctaagcga atcgttgtta ccaaagaaaa cactactatc
    35641 attgatggtg aaggaaaggc aactgaaatt aatgctcgta ttgctcaaat tcgtgcacaa
    35701 atggaagaaa ccacttctga ttacgataga gaaaaattac aagagcgcgt tgctaagtta
    35761 gctggtggtg ttgctgttat caaagttggc gctgccacag aagttgaaat gaaagagaag
    35821 aaagctcgtg ttgaagatgc tcttcatgct actcgcgctg cagtagaaga aggtatcgtt
    35881 gccggtggtg gcgttgcctt gattcgtgct cagaaagctc ttgattcatt gaaaggcgat
    35941 aatgacgatc aaaatatggg tatcaatatt ttacgtcgcg ctattgaatc tccaatgcgt
    36001 cagattgtta ctaacgcagg atatgaagct tctgttgtag taaacaaggt agctgagcac
    36061 aaagacaact acggtttcaa cgctgcaact ggtgaatacg gtgatatggt tgagatgggt
    36121 attcttgatc caactaaagt aacccgtatg gctctgcaaa atgcagcttc tgtagccagt
    36181 ttgatgttga ctactgaatg tatggttgct gatctgccta agaaagaaga aggtgttggt
    36241 gccggcgata tgggcggcat gggcggaatg ggtggcatgg gcggaatgat gtaattttct
    36301 cccggttatc caataaaaaa cccgctgtaa aggcgggttt tttattaatg tctattttgt
    36361 tctttgactg ttttgaacta tccttaataa cgtgaataaa taacaaggaa agaataatga
    36421 gtaaagtaat taaaaagctg gaagtcgctc ttgctgatac ttatgcgttg tacctgaaaa
    36481 cacaaaatta ccattggcat gttactggac ctcaatttaa aagtttgcat gaattatttg
    36541 agatgcaata taaagaactg gcggaagcag tggatcaaat tgcagagcga attcgaatca
    36601 tgggtcataa ggctcctgct acctttgctg agtttaatca attgaaaacg attaaagatg
    36661 gtgattccgg attgagtgct aatgagatgg tcagtgagtt ggcaaaagat aatatgatga
    36721 tagtaaaaga tttaaatcag gcgattaaaa ttgcccagga aaatgaagac gaaggtactg
    36781 tgactattct aagcgatcga gttgcggctc atgaaaaagc tcattggatg ttatcagcct
    36841 ccaaggaaaa gaaataaagt attcatttta tgtatcagtt gatatggaat cagtgcctat
    36901 cttcaagtaa tgggatgttt acaaaagctt caaaaaccat aaatggattt ttccatcagt
    36961 caatatggaa taatatgtgt ttgtgaaaca tccctatcaa ttatgtttat aaattgtttt
    37021 gcagagtaat aattcatcct tgtatgaacg agagtgtagt cgtaaaatga tcgattcatt
    37081 catttggcag caccattggc taaggattgc tttgtgtttc gatttttatt tcctgtcagt
    37141 ctttttataa gttccattct cctattcagt attcaaccca tggttgcaaa agcaatactt
    37201 ccaatttatg gggggacacc aggtgtgtgg actgtttgcg tgcttttttt tcaatttatt
    37261 ctgctcattg catatggcta cgtatggctt ttaagtcaga ttaagaaacc tgctgtttgg
    37321 cgtttgatcc atatggtttt ggttgtttta agttttacag catttccatt gctgtttcat
    37381 cctgcagcca gggatggaca acctgaatgg gtgattctgc ataatttgtt aattcaactg
    37441 ggtttgcctt tgttagtgat tggtgcttct gctcctttat tacaatttgc ctatagccag
    37501 acaaaaagca aaggggcctc agacccttat tatttgtaca tatccagtaa tcttggaagc
    37561 ttgggtgcct tgcttattta tccatgggtt atcgagcgat ttattggttt gactcatcaa
    37621 ttttatttct ggaactttgg atttgctatt tattcgctac tgttggtaac agtcttgttt
    37681 tttacaaaat accaaccctt aatgttaact gaaaaaaaag aggtggattt tttgccttgg
    37741 cgagagatag cctattggat ttttttgagt tttgtccctt gcagtttgat gctgggagtt
    37801 actttatata tcacaactga tgttgccgca actcccttat tttgggtttt gcctttagcg
    37861 ctttaccttt tgtcatttgt atttaccttc acagcaaccc cctttatttc acaaaaatgg
    37921 atttctcgta attgtttgtt tttcctggtt tttacaattc ttgggttcat ttttggagtt
    37981 agtcaaatca gagtgtggca attggtgtta tttaatctgt taagtttttt tattttggct
    38041 ttactgtgtc atggtcagtt atttgcacga agacccaagc catataaact cactttgttt
    38101 tatttttgtt tgtctatcgg aggagtttta gcagggattt ttaatggagt tttagcgcca
    38161 aattggttta attatgttta tgaatacccg ttagccattt tattaagttt atttgttctt
    38221 ccgctgccga aaactaaccg aggatggtgg gtgccattag ttgttttggg tttgctgtcg
    38281 cttcattatt tcattcctac cgttccctgg tttaaaagta ctaccacttt tcacattctt
    38341 gctattttgg cacttggaat tattgtgatt tggcatcgta ataaaatcaa tcttgtacta
    38401 tccatgctca ttttatttgt tttcctttac tttcctccgt tgcaagacaa acagatttta
    38461 ctcaaggaaa gaaattttta cggcgtcaag caagtcttca aaaaggatga tgtgcatgca
    38521 ttaataagcc aatcaaccct tcatgggatg caggtaatga atggggaaaa agcaagttat
    38581 ggaagaagct cctattatgg ggccatagct ccaatagttg atattttgaa gaaggaattt
    38641 catccgcttt ctgcgacgat tatggggttg ggtgcaggaa cactactgtg ccaatttcgg
    38701 gcaactgatt ccataaaggt tattgagatt gatcaacaag taattgattt agctaaaaat
    38761 aataaattat tcacatatat acgagactgc tcgccttcag ctgagattat taaacaggat
    38821 ggacgattgg ccttggttaa tatgccagat gcttcacagg atttgttaat tttggatgcg
    38881 tttaattcag atgctattcc tgtccatttg atgacacttg aagctttcac cttatataag
    38941 aagaaaattg tcgaagatgg agttattttg gttaatctaa gtaacagaca tctgcaaatg
    39001 ctcccggtaa ttaatgcaat tgggcgagct ttgaatatga taactttatt tttagcgcat
    39061 aagggagata agaagttggg gcaatttaat tcagagtggg ctatgttgac ctcaaatcaa
    39121 tccttggcgt ttcaagtgat gaaaggaaca aattggaaat ttgtagcaga tgatgatcag
    39181 tttttatgga ctgatgatta ttctaatatt atccccttgc tgaaatggta gggtaaaaag
    39241 gtaagctatc aaattcaatt gtatccttgc tttatcgtga tgcgggctat ccatagtaac
    39301 tgttcatggg gtatgtaatt aaggaatatt aggtcctctg tctttcccgc gaaggcgctg
    39361 agtgatcctg tcattatttt actcagctca aagcgccaca ttcaataaat gagtgatatt
    39421 gctgtttcaa atcactcatt atgttttgaa ataaatccca attcagtaat tccggctcat
    39481 gtcgataagc cattctagca agatgaataa aagagcgaac tcgtttgttt tttatagtat
    39541 ttgcttgata agagcgatgt ttattaaggg attcaattat taaaccgaac caccaggcaa
    39601 tcataattaa taaaacagca agaaaaaaaa gcatattaac tctggttttt cctgaagatt
    39661 gatttcgtcg taatcccatt cccagtt
//
LOCUS       DATNSN010000012        39329 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.012, whole genome shotgun sequence.
ACCESSION   DATNSN010000012 DATNSN010000000
VERSION     DATNSN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39329
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.012"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            33..4523
                     /gene="sidE"
                     /locus_tag="VAH76_000509"
     CDS             33..4523
                     /gene="sidE"
                     /locus_tag="VAH76_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SidE"
                     /protein_id="HEO1385161.1"
                     /translation="MPKYVEGIELTQEGMHAIFERMGHPNITSGTIYNGEPTIDKGAL
                     DRQGFMPVLTGVSPRQDSGHWIMLIKGQGNQYYLFDPLGESSGKYYQNILAKKLPGAT
                     LSVIPNNAGLNMGLCGYWVASVGLRAHAALTQPIPPSLRNLGQTITQEMRDELTQDGS
                     EKITQWLRAVGNEFPDGDIQPDATALRRATEKNLRIDELQPVFAGTTPKEISINPTAP
                     QETSVPTWNGFSLYTDEIVRNAARYAYDNYLGKPYTGTVEATPVNFGGRMVYRQHHGL
                     AHTLRTMAYAEIIVEEARKAKLRGESLKTFADGRTLADVTPEELRKIMIAQAFFVTGR
                     DDEESSKNYEKYHEQSRDAFLKYVEDNKSALIPDVFKDEKDVKFYADVIEDKDHKWAD
                     SPAHVLVNQGHMVDLVRVKQPPESYLEYYFSQLQPWIGSKAAEAVFAAQRQFFHATYE
                     AVAGFNSENTEPHLVVDGLGRYVIGPDGNPIREEPDDEDEEESGELKFFSQKKKLEEN
                     QRYMRVDEYLKLDEVQKRFPGAGKKLDGGLPGLNEYQYLQRLNSINRARCENDVDFCL
                     GQLQTAHHQTKITPIKRAFQSSSEKARRQPNMDEIAAARIVQQIMANPDCIHDDHVFL
                     NGQKLEEKFFRDLLAKCDMAIVGSLLNDTDIRNIDTLMQHERNTEFHSTDAKAKPVKL
                     GETWEKTIRSGGGVTQIKHDLIFLMQNDAWYHTRVNAIAQNRDKDSTFKEVLITALMT
                     PLTNKSLIDTSRSPAPKTLFRGLDLSEEFKNKLINQAETIIANTTEHLFTDLSTEAFK
                     QIKLNDFSQVSARTCASTSTNIAVPRTIFGSNTIFEILDPDGLLHPKQVGTHVPGSES
                     EYSIYLPEDVALVPIKVSFDGKTVKGKDRHIFTLVAVKSPDFTPKHEIGYAVGPLLRM
                     QTPKLEEIQRLVEQAREEPDLERVFNLQSRVARQAKLFSTEPGYKTFLNEKVAPVLEQ
                     SLNGLLDNNVTILGKVLSAFPSDGQWSAFNSVEARQMKIQMDAIKQMVEKKAVLEGRI
                     LPALAQCQDALEKQNIDGALQALKNIPSEKEMQTMLSISSGLRGQIQRAKQNLTENLE
                     PLQRAMTAKLVSDKEKVKVRYEKLIAGIPQQITDLEKAALSDLAGVKKVVSHFNHLQE
                     ELKLLRNEKVRMHTGSEKVDFSDIAQLEAQLQKIHTKLYDAYLVELTKEISALAKEKP
                     KNLSDVKRMVSNFYAMSADIEQLRQEKIKEHGESKDPIDMSDIDKLKKELQKINQFLV
                     KAMGTNIRVSLNQMEVKTFDAQEKEAQQNLKQLDELINKLESSDAVQKQKEELEKLNQ
                     LLIEKRKAYPAMVQLQFRSEALIIHLRELCEAHQAQMAKTRKARAQEITNGRWKVQWL
                     TDWVGLTTDERVTLANKEKELAKFKEDLNNDEYDLQELISNLAEKTPSELEEAIGISK
                     ESAQKLHKLLTHLNHSTTFMSKIEQRLQSIDELLNEFGKQSPRTEMIKTVEEKQGTLL
                     RL"
     gene            4819..6411
                     /locus_tag="VAH76_000510"
     CDS             4819..6411
                     /locus_tag="VAH76_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEO1385162.1"
                     /translation="MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATY
                     FLTLQESSTVGIAAGYAMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQH
                     SRPYLIHSPVMSGDLTSLAQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
                     PMDIWQEETHETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIY
                     LLHTIADRLNADIYAAPYHVREVINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEK
                     VKGYLYTGQSSIPQGIQFIQISAASEHLAFDYPCDLAILGNLEATLTAISKHLKINGV
                     SVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAVVQDIATTLG
                     FLNVNFSPRGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVI
                     FICFVNREYRVLKTDMSQNRSFIEEDFFRLDLNHPQIDVQQIALSFGAQVKAIKSKEE
                     VETVLKSSFAFQGPTFITLYHQ"
     gene            complement(6529..6975)
                     /locus_tag="VAH76_000511"
     CDS             complement(6529..6975)
                     /locus_tag="VAH76_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEO1385163.1"
                     /translation="MKHNLLDKAYKHCVNHGYRFTEPRERVLKILVDERKPLGAYDIL
                     QRLSMEVDNPKPPTVYRAIQFWHQEGFIHCIDSLKSYVACLHGHHVGQAQFLICNQCD
                     FVKELECVVDFTSVTEAANSIQFSIINCTVEIKGLCSDCNLTNLKN"
     gene            7307..9865
                     /locus_tag="VAH76_000512"
     CDS             7307..9865
                     /locus_tag="VAH76_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO1385164.1"
                     /translation="MLYSNYRFYLAKDAQLLNPEESREAIEQILAKTKELELIRVAVD
                     LTSPNKSICLLSASKESHVIKTKVTQILKEQGLSIIDMMEVEVLQDIEKIEHHHPTHK
                     SKDTHLHPIKKHPKKAKHDHSHGHGHHHGHHHENHWIKAALGLIWGIGLLVLSIGTFN
                     IPLVAYYVITGLTSLMTLYLGHTVYQSAWQALKEKKWDTTTLYSISTLTILAVSIASV
                     FVPGLPMMFEAAPLVLGFWHLGEGIEHTLVGKINKKLDVRDCVPPLVLLKGNPDKEIS
                     VKTLIPNDMIIVKKGQVIPVDGVLTQKALLYTTRIDGSPKLKEFHPGDVVKAGMQLAD
                     HIPSLEMQVTKTYQNSYLSLIAENINKANDEKAPIEMFANKVLKYFIPGLIAVALLSG
                     IVIGSLFNPALAIQCVVSVLVSACPCALSLITPMAVKIGMKKASENGIHFNNGKALQA
                     AADIDTVVFDLNGTLTQGKMVVKSLHITDKKYLRPIALLESQSDHPVAKIIKSYIEQQ
                     DTVSTEPLEIASIDKSHHSGMKGIINGEAFMIGNKDMLAANGITSIDKPYDNPEKGSV
                     YIVRGTTVIGQIALIDPLREDAIATVKQLKRLGKSVHICTGADQATAEKYAALLGIPK
                     DNICANTVGAVTKKGEVSKTSYIQQLKQKGFKVAMVGDAANDATAIAYSDIGIAVKSS
                     IGDSITEQHAGIVVQQGLLFPIATAFDVAQKTKQNIFQNLFVSLSYNSIITLVAAGLF
                     VALGFALNPVLGVALMVLESTIVLTNLYLFKQQDIVSSTSPNANNAVEVEPTVNTTSK
                     VLHALGHRSQPQENLAIVTESKLVNPKVINLFAPKELSSNRPDVTQQKLGCVTC"
     gene            10010..12153
                     /locus_tag="VAH76_000513"
                     /pseudo
     CDS             10010..12153
                     /locus_tag="VAH76_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            12365..13198
                     /locus_tag="VAH76_000514"
     CDS             12365..13198
                     /locus_tag="VAH76_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEO1385165.1"
                     /translation="MFSKALISATYKNGKPGQLTEEHEKAEHHRFKTEFLFKNAEISK
                     ELYASRLIQHFFIIKAIETKLQSLSKTEQSEISAFFAISYLEHLWRSPAIENDLRQLN
                     INPDEIDKDEITKTTENYLENIGKFTPKILLAHFLLHIAGFMHGGNIIRSKYIEPSNR
                     LTSYQIAADQYDFSSAISFLSSGRHSPLALYQDMMKQIDNIALTDGEYDEILEQCKGI
                     YETMSNIYDDLCDMHAHHLKLPTYSLAAISVSLVALGLILKLLMDFLNPVTNPIANAP
                     R"
     gene            complement(13613..14719)
                     /locus_tag="VAH76_000515"
     CDS             complement(13613..14719)
                     /locus_tag="VAH76_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1385166.1"
                     /translation="MSYSIDNPGGGDCGFYALAIGLISIIQNEYKLHGKSKTYDRWKQ
                     VEGIYGVRLQDILNVDLTKLHDSPYTYKSELLFLLQMSLRNIAVIVNKEDLIKRIIEE
                     GRSKDQTKIEGSNAYCKFMELVQLYLYRRDSLARISQFNELALSPEIVQTAQDIARIL
                     GPILRNQPFHQVQKIENAFVKEVLLNDVLSGNTINSNSIILKSLEKIKEQGRWATHDD
                     LKEIADLLKINLNVVGQPNGKPLPEYPTVTLKNLSNTHWITQVESLQGPRQYSTPRKR
                     AFGYETVSDRAVPLKEEQTLDRTFRTQTQSERAKRYRQHFETLFDTLSPYGFFTYEQN
                     KRNTQVSGKVQTGLESDESLAARLQEAELRRILY"
     gene            complement(14902..16299)
                     /locus_tag="VAH76_000516"
     CDS             complement(14902..16299)
                     /locus_tag="VAH76_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1385167.1"
                     /translation="MICFPSGKNRLMVLCLFLMLSFKCFPIPAKPLLLDAVLKSVDRS
                     YPQIVIARLEIRKAEGDYVNALGKFDPSFDVNPRFQPVGGYINNYADNLFNVPTLYNG
                     LKLFGGYRIGRGDWPIYYQNYLTNSGGEYRAGLSLPLLRDRRIDKERTELFTREETIR
                     MKQQDAAATKIKIYQETIKAYWQWVEAGFQLRTFRHLLNLAKERQNAIIQQAQQGDLP
                     RLAIAENLQLIVQREQLVNQGEMLLAQAGINLSLYYRDANGKPQKPDESQLPATFPAQ
                     PIRVSNSISQLQRHPVLRKLENYSKIVKLKQNLARNELLPNLDATAYTFKQYGTGGYP
                     LLIPQAAMIGVNFKFPIFQREARGKLISTTSELQQVKTEMKFTYEQLNNELSNLLVAI
                     RIYQQQVVLLNKELNLARQVEKGETKRFYEGDSTLFLVNQREQTTAQVKLNWINAEVN
                     LQEATALARFFSSTQ"
     gene            complement(16296..17282)
                     /locus_tag="VAH76_000517"
     CDS             complement(16296..17282)
                     /locus_tag="VAH76_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEO1385168.1"
                     /translation="MKLHAYSMISKLPKPQKIAKIIFIVFVAIILFLSFTPWQQFALG
                     NGKVIAFSPTERQHTVNSPVSGRIKKWYVDEGMRVKPGDPIVDITDNDPELLSRLEVE
                     KKAILLRIEAAKQAIAAGKANVDRQKKLYEQGINSRRQYELAQIEYAKYQNDLAQANI
                     DKVNVDVRIARQKTQIIKAHVAGIIFRRLTGQESVVVNAGDVLAQILPETESRAVELW
                     VDGNDIPFVRLNQKARLQFEGWPAIQFRGWPEIAVGTFGGTVSFIDPTDNGFGLFRVV
                     IVPNEPWPSVRFLRQGVRVHGWIQLGQVPLWYELWRQYNGFPPESNRENKAS"
     gene            complement(17279..18868)
                     /locus_tag="VAH76_000518"
     CDS             complement(17279..18868)
                     /locus_tag="VAH76_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385169.1"
                     /translation="MNAKPINSIIDVLDRQTISSIAMISVIISLLSLSIPIAAQTLVN
                     LIAFGKLLQPVITLSLMVLVLMMALGALHIWQIVIIEVIQQKLMVKVSLSLTRHFTHM
                     SLDNFSTHHGPELVNRFFEIVTIKKSLASLLLYGINLGLQLFFGLLLILIYHPLFLLF
                     DIFIIIGILLIIFIPYKKALDSAKKECSEKHVVGAWLEEILINRYLFRFNLHHRYVTQ
                     QTDKKLVAFLKARNTHFKQLIKHQIGFYMLSALASSLLLGLGGYLVINNQLSLGQLVA
                     AEIVLGALIYSFRRFGALLENYYDLVASENKIDAVLNLSLEQVKEDEHGELLTPINSI
                     QLEMDSKNKALIAVDKPLLVFSEKSDLCQLFVEQLFGFQDPVSLHVLINEIPCAKRTL
                     ISLRRFSLLIAEPQWFTGSIYDNLVLNHRNMSNKIIIEQLQNVGLADKIIQLPEGLHT
                     IVYEWQDVFTSVELTKLMLVRALLLQPELIVIDRAFDILNHQEVNEMMELVLSLKNTL
                     LLIVSQRSDFNYLNNRLVISS"
     gene            complement(18910..19761)
                     /locus_tag="VAH76_000519"
     CDS             complement(18910..19761)
                     /locus_tag="VAH76_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1385170.1"
                     /translation="MSIDTITLQCFLAVAETQSFTKAAQRVGRTQSAISQQIAKLENL
                     IEKPLINRGRELSLTTDGEIFLGYAKRIYELHRESLDRFKAPELQGEIRFGLPEDFAS
                     IILSDVLVEFSRLHPRVLLNVECDLTLNLIERFNEGKFDLILIKTTQQQVGSESIHVC
                     TEPVEWIGKKELLPLLDKPGPIPLVLSPTPCVYRGNVIDTLDKNHIDWRLVYSSASYA
                     GKMAAVRAGLGITAIQRSMIPNYLERLDYDFLPVLNDVHVSLLKKSDSNKAIESLEFF
                     ILQKLKH"
     gene            19863..21383
                     /locus_tag="VAH76_000520"
     CDS             19863..21383
                     /locus_tag="VAH76_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEO1385171.1"
                     /translation="MDKLLNGLSISLMLSPLIAMLFYSLLGKTRNIQATYYGTTVIGF
                     GFGLSLLALIVSANTVNQNSYFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCY
                     RRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
                     KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSPAIAVALGFIILTAMTQSAI
                     WPFHRWLISSLNSPTPVSALMHAGLVNGGGFLIVKFAPLFVMQTNLLTILFIFGALTA
                     LLGTLWKLLQWNIKSMLASSTMAQMGFMIMQCGLGLFPAAIAHLCWHGLFKSYLFLNS
                     GSAVQQKKYQNKSRPEKLTTLLTAFFGGLLGMYGFALITGKPILSLHASTFLLGFAFI
                     TGTQFTLGLIAYEKHFKKVILALALVFSIGIFYGGSIHLIESLLPDLTGFPQHQLSSL
                     QVLTLIVFFSLWLVFSMGLFKKIRETRLWDWFYMYMFNASTPHPKTITANRHTYYY"
     gene            21398..23686
                     /locus_tag="VAH76_000521"
     CDS             21398..23686
                     /locus_tag="VAH76_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEO1385172.1"
                     /translation="MSSHITLIKTNTNNENQMTQFNYKVDNDVMIIRAMVNNVAKQIT
                     PVWPLEKFIACNSLHGFESMSFEEAVIQNQTAKKGTPFNEKLERVNWHMIKWCGSFLD
                     IGQGTLEMPHRDKGLYFGFLKLAPFDSELHQNNKSTKSWLSNLPEMPEQAIRLCLNKL
                     GVLNQKQEKFLKSTLSHLPGWAGYIKWISEWKNRNGKEENPVSLADFLAVRLIITCVL
                     WPEASQEEKKKKKDNAYTKQLIQNIKNKEDDYRQLLLNKLLPELDKVQIKENRANAQM
                     VFCIDVRSEPFRRCIEKLGHYETLGFAGFFGLPVSIKDYDGETIKDSCPVLLKPRFNI
                     HEKAIAANEHCIEHHEKGKEFKNILNRIYQQLKYNFSTPFALVESLGIWCGITMFLKS
                     CSPIFARRLTKDLNEMICPSIQTQPVFELDLLEKEVGISLQEQIAYAEMALRLMGLTD
                     NFAKLVIFCGHGSSTQNNPYASALDCGACGGNQGGKNAQLLASILNKIIVRRALAENG
                     INIPQDTLFYGAQHDTTTDEVEIYHSNVSQFIHQDILDQLRTDLNIAKHNNNLERINY
                     LNSIDCAEKDIARRSTDWSETRPEWGLARNAAFIVAPRQLTKNINLEGRCFLHSYDWS
                     QDKDGALLETILTAPMVVAQWINTQYLFSTIDNVAYGSGSKITHNVAGKIGVMQGNAS
                     DLMHGLPLQSVMSHDDKSFHEPQRLLTLVYAPREIISELVEKHDVLKTLFFNEWVHLV
                     AIDPRSHLFYKLEKTNTWSVIQ"
     gene            23700..24053
                     /locus_tag="VAH76_000522"
     CDS             23700..24053
                     /locus_tag="VAH76_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEO1385173.1"
                     /translation="MNIIKVSEKKEMNISLHPMKKIEVIVSGDNENMVATMMEKANIS
                     GFTLLRNVSGKGHHGFHEGKVLFNDKASFVMFIAVASEEAIANIALGMKTLFQKNSGV
                     LFVSDVSVARVDYFS"
     gene            24078..24917
                     /locus_tag="VAH76_000523"
     CDS             24078..24917
                     /locus_tag="VAH76_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385174.1"
                     /translation="MHYQNQRVLLASKHQKEKAIQPPFEEKLGCRIHVPADYDTDQFG
                     TFTGEIPRKASAYETLILKAKAAAKQFGYRYVISSEGSFGPHPQLYFCPADTELMAFV
                     DLDNDLIIAEHEVSTETNYSHIDISATDDYEAFLNKAKFPSHGIIVRALNSEENYIQK
                     GICEREQLKTAIQKAFTYSTTIRLETDMRAMMNPLRMNVINKLAVKLVHRIQQYCPQC
                     HTPGFGKISSEGHLACISCGTPTELYLRKVLNCLKCEFKTYQARDDGLEFADQRYCPY
                     CNP"
     gene            24977..25477
                     /gene="purE"
                     /locus_tag="VAH76_000524"
     CDS             24977..25477
                     /gene="purE"
                     /locus_tag="VAH76_000524"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO1385175.1"
                     /translation="MKPILIGLIMGSQSDWQTLIHAAQTLDALNIGYEAEIVSAHRTP
                     DKLFQYAEQAEERGLEVIIAGAGGAAHLPGMVAAKTSLPVLGVPVMSQTLNGVDSLLS
                     IVQMPAGIPVGTLSIGKAGAINSALFAAAILANKYPDIRAALKRYREQQTQKVLDNPN
                     PKEEKS"
     gene            25474..26553
                     /locus_tag="VAH76_000525"
     CDS             25474..26553
                     /locus_tag="VAH76_000525"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO1385176.1"
                     /translation="MKLGILGGGQLARMLALAAYPLGINTVCLDPSPDACAGQLTKLI
                     VSDFNDELALKQFLSEVDCVTIETENIPFSCAELAAQIKPFYPSTQALKITQDRLYEK
                     NFFRTLNIPVPEFLPVESEDELMQAISVIGMPAVLKTRRFGYDGKGQYVLRTQADISK
                     SWSLLGSQALILEKLISFESEFSLIAVRNKSGETSFYPLIKNHHRKGILHFSEAPVHQ
                     DTLQQEAQTHAFKILEALNYVGVLTIEFFYQGNQLIANEIAPRVHNSGHWTIEGAHTS
                     QFENHLRALFDLPLGSTEVSGSCFLLNCIGNLLPLEPCLSIPGVHYHAYGKAPRPDRK
                     VGHVTLVDTDLERYTKSKDLLLDIS"
     gene            complement(26639..26851)
                     /gene="yacG"
                     /locus_tag="VAH76_000526"
     CDS             complement(26639..26851)
                     /gene="yacG"
                     /locus_tag="VAH76_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEO1385177.1"
                     /translation="MNNQKKIKCPICGKQNTWRPDNQFRPFCSERCKLIDLGEWASES
                     RKIPGSSIDPESIVTTNNKQDNVDEQ"
     gene            complement(26973..27857)
                     /locus_tag="VAH76_000527"
     CDS             complement(26973..27857)
                     /locus_tag="VAH76_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1385178.1"
                     /translation="MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELG
                     ITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGS
                     STLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
                     SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVA
                     QSSQIDFMLELVAAGLGVTFLPRMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYL
                     SEAAKAWLALVREVHVDN"
     gene            27963..28352
                     /locus_tag="VAH76_000528"
     CDS             27963..28352
                     /locus_tag="VAH76_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO1385179.1"
                     /translation="MANKIQQNSFFQIILVGLFWLASELLLKLVKLPLSGGILGLGIV
                     LLLLATNCLKLNRIRRGAELLLANMLLFFIPGVLAILEHQEFIGLLGLKILFIILLST
                     ITVMLVTALVVDYCYRWRTHHAKQHSL"
     gene            28330..29037
                     /locus_tag="VAH76_000529"
     CDS             28330..29037
                     /locus_tag="VAH76_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO1385180.1"
                     /translation="MPSSILSESMIQAFFWSLITIGMYFITQKLYRRWPFWWLMPLAL
                     TPILVASIVLLLHVNYQDYFRGTKWLVLLIGPATVAFAIPIYEKRALIRQHWPVLLIG
                     VIVGSFTAIASSWGLAILLGLDDNLRLSLLPRSISTPFAIEISRGIGAAPDLTAIFVI
                     VTGILGAILGEFILSRLPFNSALARGALLGVGAHAAGTAQAHKIGNTEGAIASLAMIL
                     VGLFNILLLPLISYLLK"
     gene            29132..30547
                     /locus_tag="VAH76_000530"
     CDS             29132..30547
                     /locus_tag="VAH76_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEO1385181.1"
                     /translation="MTIALVWFRQDLRLTDNPAFIEACSHHETIIPLYIYDDKCSVLG
                     QAQAWWLHHSLIALGKSLNQQGLSLVLRKGSPLEIILELVTQYGLESVYWNRCYEPQV
                     IARDKKIKLALGEKGIKVHSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
                     PFMLTKRPRGIEITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLNEFIIHHLSG
                     YKRDRDFPAKNATSRLSPHLHFGEISPWVILRALELTKLEQTCDLASVEHFLSELGWR
                     EFSVYLLYHFPKLDCENFRNEFDAFPWQNDEQLLTRWQKGMTGYPLIDAGMRELWATG
                     YMHNRVRMIVASFLTKDLLIDWRLGAQWFLDTLVDADLANNSASWQWVAGCGADAAPY
                     FRIFNPVLQSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDASASSIFLGNKYPKPII
                     NHQEARTKALSYYQQIKKGMD"
     gene            30580..30678
                     /locus_tag="VAH76_000531"
                     /pseudo
     CDS             30580..30678
                     /locus_tag="VAH76_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
     gene            complement(30713..31336)
                     /gene="ravG"
                     /locus_tag="VAH76_000532"
     CDS             complement(30713..31336)
                     /gene="ravG"
                     /locus_tag="VAH76_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavG"
                     /protein_id="HEO1385182.1"
                     /translation="MKIIYPGRVICDMKNAAIIKNRIFLNLDKPVKRFLASDKADTPM
                     TAEFYAEKDYEQLFLDFLSQATGSYDEQISMLIAELDSGADRVAQKLMSALYSPWQKN
                     LFPKAIKTIANKSEEYPLMSDLLIKFCQQHVGSVDAVDDFGETALAKILKKDQQKKSP
                     LLFLVKHGAKHCQLTSALQDSLIVNNPDIYNVAEDNTMDWVSNRSQA"
     gene            complement(31381..33351)
                     /locus_tag="VAH76_000533"
     CDS             complement(31381..33351)
                     /locus_tag="VAH76_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385183.1"
                     /translation="MLSKEKINPSLKNTKRQGSDNLAAMADLTSLKNTGDYRCFNSKP
                     EAYTYFIFLLNQWQTNYMGDKREAIAKAIDSIKMAYENHEQDTSVVHKMLHGLSHLLE
                     QEHADELNALCLVPIKDSQSNANNILPRRFESELACRLIESPTKSMMQAIDLVSQAII
                     NLIEEYSKLDSKSSERAALNFFLQELEGSDIEFGAFKKRPSLEEVIAILKENNPKNFI
                     QIQFISFMFARSVIGPFPLRNLPSFKPTGLFAQVLDKYLQDHPDLKEEEEGFSYQLIA
                     AKNEAEEFRTFITKKLFYQDELYTQGENRGRQGPRKTYLKTNQLGLMKLDHSAHSRGL
                     TVIPEQTWCADAQAQEANQNSIYYKTALTNDCPYVTGPSGMTSIYMNMMLLLLNPQNI
                     ELIQAYSLGIIAYIVGAGFHSVNEILIPLVKCINLLPNYPSYEGLEYLTHPPLYHEYF
                     KQIAQFDSDFIQLRDQVWAYYLNYFKSCYMPLCMRDFCEAEQLANCPAPAPEVKILCD
                     IVLKSIEDCINFHNLNRHLDSRFLGTTTADIDALNQLKEACASQSSISGIMRLLQKYF
                     SGENIARPTAMHKFETRSYIRFFLDALKDNPQIISHINEKMKLDPPLIISTKAEVFSD
                     DFRIECFNMHRILKREHQVSEDCNNTSQFLKL"
     gene            complement(33370..36255)
                     /locus_tag="VAH76_000534"
     CDS             complement(33370..36255)
                     /locus_tag="VAH76_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEO1385184.1"
                     /translation="MKLLRFHELKSLPSMDEKALELLIKVLGNKGIRKLIKSADGKPI
                     SREIMIHEFGIDCQILFITTGASLKPIIVPTENKISGGGKSYCEQFKVYALDDGKTYF
                     LKSVKIDAESVTEFTNEKDTLSKLGRLVGTFFNEQTQVHYILTTFIKGIDLSRYKNAL
                     PLSVNLKHFWEVLGIMISVCHQVKQFHELGLIHRDLKPGNIMLDADMQCHLVDFGSSS
                     SDKEPKPVSWGTASYLAPELNAQEDFIAFSQASDLFALAYSLDELFNPFRQVKFAKVD
                     IGIKNKHLVLLHAEIEACITGLMSNETSARTLYFSRILQLQRVPESFKSRPEAFTYMI
                     MLLTQWKSCYEIPELKKELDEIIAEIKVAYENHEQDVLKITSMLEQLSKADGLLNSHK
                     SLLSVLIKSLANVQQQELGHDDILPRRFESDIVSQLIKNPTAKMMAAIKQVSDAIVKI
                     LEQYEHSPTESFEHEVLQDFLEQMVQSDILFGAPKEKIEISDIIKILKENRPEDFVQI
                     QHISFKFAQVALRDLALHDLPKHEPEGAFLKILQKYQAKYAPEETSLFELYHHMAAVR
                     DHSASKVKKGEGYRFRAFICDELFYGDEIFSTGDNRGREGKPNQRLQTNQVGLMKFEH
                     SVHTKGLLTLSGQSWYADCKTQLPNYDSIHYLSALKTDCPYITGPSGMTSLFMNMMFL
                     LLNPKDQDVILSYSLGVMTYVVGAGYHSIKEILIPMVKCVGIVPDYPQHKGTEYLTAS
                     PLYNHYFKAIEEFDKEFAEVHEKIWQEHLAYFRKTYMPVCMRNHCLPHQIPSMEDISS
                     EVKEMLEIVSKSVKNCLEEHKVKRTGDMGLFLSAPCGQTKALEVLSHVCQQQVGITAI
                     FRQIQAYFKGTFETEEGIVINKEMQLKFIAHFFNVLKESPTLLSQFNLTLRIENTIGV
                     HQCEIGSSRRKELFLEIKKTSIAANEEQMVANHEPRKENRNVIILPY"
     gene            36629..37042
                     /locus_tag="VAH76_000535"
     CDS             36629..37042
                     /locus_tag="VAH76_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEO1385185.1"
                     /translation="MNGWTLKVFAIAFFIFIVTDMIWLGFIARNLYFDQYQQWLRLSE
                     GQLKPIWWSALLVYLLFALSIVVFVIPLAKTSLPYAALYGALLGAIIYGIYDFTCLAI
                     FKDFPVGMAFLDWLWGIVLCSWSSIATIYVAGYIK"
     gene            37301..37837
                     /locus_tag="VAH76_000536"
     CDS             37301..37837
                     /locus_tag="VAH76_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HEO1385186.1"
                     /translation="MNINPLNLVHYVAYYLCWIFCVYLASYGYPLLGLIITLLIMAVQ
                     IIVQILNQLSWRDALCFSIGLAILGAITDAIWLYSGMIYFKANFFGSHFPPLWMFALW
                     LSFGFNLVLLYSSWLTHYFLWGLVMFPSIIFAYWLGVQFGAAILIGNEIVFYLTLGLT
                     WSLFLPLSLYLFNYQYRK"
     gene            37834..38610
                     /locus_tag="VAH76_000537"
     CDS             37834..38610
                     /locus_tag="VAH76_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEO1385187.1"
                     /translation="MNKMIILLVIVIIFLHMCLIWLWYRYTNNPSVVDVGWASGLTLA
                     GLVYLNNQEPSLRFLLLSLALLIWGVRLGGYLWWTRIRLKKIDKRYLALSNDWKLAKP
                     LGFFLNFQLQGALICLVSIPWYFSSSGTQTTLSLLDILALIIFLMALILETVADNQLQ
                     DFKKNYPGKVCNQKLWRYCRHPNYFYEWLVWCSFTLFALSAPYGWIAIISPLTLYLIM
                     TKITAPMTEQGSIESRGNLYIEYQKVTPMFFPKWLKIIRW"
     gene            complement(38819..>39329)
                     /locus_tag="VAH76_000538"
     CDS             complement(38819..>39329)
                     /locus_tag="VAH76_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385188.1"
                     /translation="PDNLPKDFSSDEYFYSVLDNIGYLKKIFQKTWVIQYVNFFPDEW
                     NNDHHYMSRLFAFARKHHIGLGGPDVVPYKESQMKNSYPFFHKFKGKIETAMAIQEPD
                     YTYTNPKTGAFYRFDDFYSFSKEYLGAFILFWNTEEPFFSEQLLPKLNSVYFKCDKVN
                     NEVNAADIT"
BASE COUNT        12140 a         7781 c         7606 g        11802 t
ORIGIN      
        1 aatttaaaaa gtatttatcc aggagttgtg taatgcctaa gtacgttgaa gggatagaat
       61 taacccaaga agggatgcat gctatttttg agcgcatggg acatccaaat attaccagcg
      121 gtactatcta caacggagag cctacaattg ataaaggtgc tctcgacagg caaggcttta
      181 tgccagtatt gaccggggtc agcccaaggc aagactcggg acattggatt atgttaatta
      241 aaggtcaagg caatcaatat tacctctttg acccgctggg agaaagctca ggaaaatatt
      301 accaaaatat tttagccaaa aaactacctg gcgctacgct ttctgttatt cctaataatg
      361 ctggattaaa tatgggatta tgcggctatt gggttgcctc agttggttta agagctcatg
      421 cggcattaac acaacctatc cccccctcat taagaaatct gggtcaaacc attacccagg
      481 aaatgcggga tgaactaaca caagatggaa gcgaaaaaat tacacaatgg ttacgcgccg
      541 ttggcaatga atttccagat ggtgatatac aacctgatgc caccgcttta agacgtgcta
      601 ctgaaaagaa tttacgcatt gatgaacttc agcctgtttt tgcaggaaca acacccaaag
      661 aaatctcgat aaatcctact gctcctcaag agacatccgt tcctacctgg aatggttttt
      721 ccttatatac tgatgaaatc gtgagaaatg cagccagata cgcgtatgat aattatttgg
      781 ggaaaccgta taccggtaca gtagaagcga cacctgtcaa ctttggcggg cgaatggtgt
      841 acagacaaca ccatggtctt gcccacacat tacgcaccat ggcttatgcg gaaatcattg
      901 ttgaggaagc gcgtaaagca aaattaaggg gtgaatcatt aaaaacattt gctgatggtc
      961 gtactctggc agatgtcacg cccgaagaat tgaggaaaat catgatcgcc caggcattct
     1021 ttgtcactgg aagagacgat gaagaatcct ccaaaaatta tgaaaaatat cacgaacaaa
     1081 gccgagatgc ttttttaaag tacgtcgaag acaataaatc tgctttaatt cccgatgtat
     1141 tcaaagatga aaaagatgtc aaattctatg ctgatgtcat tgaggataaa gatcataaat
     1201 gggcagattc tccagctcat gtcctggtca atcagggcca tatggttgat ttagtcaggg
     1261 taaagcaacc cccggaatct tatctggaat actatttttc gcaacttcaa ccttggatag
     1321 gctcaaaagc agctgaagcc gtttttgcgg cacagagaca attcttccat gccacttatg
     1381 aggcagttgc gggttttaat agcgaaaaca cagagcctca tctggtggtt gatggcttag
     1441 ggcgctatgt gattgggcct gatggtaatc caatacgcga agaacctgat gacgaagacg
     1501 aggaagagtc tggtgaatta aagtttttct cacaaaaaaa gaaactcgag gaaaaccaac
     1561 gttacatgcg cgttgatgaa tatttaaaac ttgatgaggt ccaaaagcga ttccctggcg
     1621 ctggaaaaaa actcgatggg ggactgccag gactgaatga atatcaatat ttacagcgct
     1681 tgaactcaat aaacagagcg cggtgtgaaa atgatgtcga cttttgtttg ggacaactac
     1741 aaacagctca tcatcaaacc aaaatcactc caataaagag agcatttcaa tcgagttctg
     1801 agaaagcgcg aagacaaccc aatatggatg aaattgctgc tgcacgtatt gttcagcaaa
     1861 tcatggccaa tcctgattgc atccatgacg atcatgtctt ccttaacggt caaaaactgg
     1921 aagaaaaatt ctttcgtgat ttattggcga aatgtgacat ggctattgta gggtcgctat
     1981 taaacgacac agacattaga aatatcgata ccttaatgca acatgagaga aacacagaat
     2041 ttcactcaac cgatgccaaa gcaaaaccgg tgaaactggg agaaacatgg gaaaaaacca
     2101 tcagatcagg tggcggtgtt acccagataa aacatgattt aattttcctc atgcaaaatg
     2161 atgcctggta ccatactcga gtcaatgcga ttgcgcaaaa ccgagataaa gattcaacct
     2221 tcaaagaagt gttgattacc gctttaatga caccattgac taacaaatca ttgatagata
     2281 catcgcgttc tccagcccca aaaacgctat tccgtggctt ggatctgtca gaagagttta
     2341 aaaataaatt aattaaccag gctgaaacca tcattgccaa tacgacagaa catctcttta
     2401 ccgatctgtc gacagaagcc tttaaacaaa tcaaattaaa tgactttagc caggtttctg
     2461 ctagaacttg tgccagtact tccactaata ttgcagttcc caggacaatc tttggctcca
     2521 atactatctt tgaaatactt gatcctgatg gtttattaca ccccaaacaa gttggaaccc
     2581 atgtaccagg gtcagaaagt gaatattcca tttatttgcc agaagatgtt gccttggttc
     2641 caatcaaagt aagctttgac ggaaaaacag ttaaaggcaa agaccgacat atttttactt
     2701 tagtcgcggt aaaaagccct gatttcacac caaagcatga aattggctat gcagttggtc
     2761 ctcttttgag aatgcaaact ccaaaattag aagaaattca aagattagtt gagcaagcaa
     2821 gggaagagcc tgacctggaa agggtattca atttacaatc gagagtagca agacaagcca
     2881 aattattctc cactgaacca ggatataaaa cgtttctgaa tgaaaaagtg gcgcctgtgt
     2941 tggaacaaag cctcaatggc ctcttggaca ataatgtaac cattctaggc aaagtattat
     3001 cagcattccc ttcagacggg caatggtctg cctttaattc cgttgaagca aggcaaatga
     3061 aaatacagat ggatgccatt aagcaaatgg ttgaaaagaa agcagttctc gaaggtcgaa
     3121 ttctccctgc cttggctcaa tgccaggatg ccttggagaa gcagaacatc gatggtgcat
     3181 tacaagcgct taaaaacatc ccctctgaaa aagaaatgca aactatgctg tctattagca
     3241 gtggcttgag aggacaaatc cagcgtgcga agcaaaattt gactgagaac ctggaaccac
     3301 tacaacgtgc catgactgcc aaattggtaa gcgataaaga aaaagtgaaa gtgcgttatg
     3361 aaaagctgat agctggtata cctcaacaga tcactgatct tgaaaaagca gcactctctg
     3421 atcttgccgg tgttaaaaaa gttgtctctc attttaatca cctgcaagaa gaattaaagt
     3481 tgctgcgtaa tgaaaaagtt cgaatgcaca ctggctctga aaaagtcgat ttttcagata
     3541 tagcacaatt agaggctcaa ctacaaaaaa tccatactaa attatatgac gcctatttgg
     3601 ttgaattaac taaagaaatt tcagcactag ctaaagaaaa accgaaaaac ctgtccgacg
     3661 tgaaaaggat ggtttcgaat ttctatgcta tgtcagctga tatagagcaa ttgcgtcagg
     3721 aaaagataaa agaacacggt gagtcaaaag atccgattga tatgtctgat attgataaat
     3781 taaagaaaga actacaaaaa attaatcaat ttttagtgaa ggcgatggga actaatatca
     3841 gggtttcact gaatcaaatg gaagtaaaaa cttttgacgc gcaggaaaaa gaagcacaac
     3901 aaaatttgaa acaattagat gaactgatca ataaattgga aagttctgat gctgtacaga
     3961 aacaaaaaga agaactcgaa aaattaaacc aattgcttat cgagaagcga aaggcctatc
     4021 cggcaatggt gcagttgcaa ttcaggagcg aagcattaat tatccattta cgtgaattat
     4081 gtgaagctca tcaagctcaa atggctaaaa caagaaaagc aagggcacaa gaaatcacca
     4141 atggacgatg gaaagtgcaa tggttgactg attgggtggg tttaactaca gatgaacgag
     4201 taactcttgc taataaagag aaagaattgg ctaaattcaa ggaagattta aacaatgacg
     4261 agtatgattt gcaggaactc atcagtaacc tggctgaaaa aaccccgtca gaattggaag
     4321 aagccatagg gatatctaaa gagagcgctc agaaattgca taaactgtta actcatctaa
     4381 accacagtac aacatttatg agtaaaatcg agcaacgatt acagtctatt gatgagttat
     4441 taaacgagtt tggcaagcaa tcgccccgaa ctgaaatgat aaaaactgtt gaggaaaaac
     4501 aaggaactct cttgagattg taattcttta tcttccacag tagcccatta agggctaccc
     4561 cgtgggatat tttagatgat tacggaaaat aaaattataa ttatcaacgc ttgccgggtt
     4621 taaaatgcaa ggacatatct aatcgctgcc atctaattta tttttgctcg caacgcccca
     4681 tcacaatatt taccacagca ttagctccta aaaaccatat ttgccctatt tttaatgaat
     4741 gagagaaatt gaacaaatag ctctcttgtg ctaggaatat aaatatggaa gtgttcctat
     4801 ttctaaggat gcttatcggt gaagaaaaca ggaagtgatg ttctcaaaga atttataact
     4861 acctttgaaa ttaattatat ttttggcaat cctggcacta ctgaattaaa atttctggaa
     4921 gcgattgagc aatgtgataa tgctacttat tttctcacac ttcaagaatc cagtaccgta
     4981 ggtatagcag caggctatgc catgatcacc aggaaaccgg ctcttattaa cttacataat
     5041 tatgttggat tagcgaatgc tgtctgtaat atgtacaatg cattcacttc aggaattccc
     5101 ctgctaataa ttgctggtca acattcacgt ccctatttaa ttcatagccc agtcatgtcc
     5161 ggagatctga cctctttagc gcaaactgca gtaaaatatg cctatgaggt gaattccgcc
     5221 agcgatctgg ctgttgcttt acagcgtggt tatgctcagg caattttgcc tgggccaggt
     5281 ccggtttttc tatcaatccc tatggacatt tggcaagagg aaactcatga aaccacgata
     5341 aagaagacaa gatttttaca tgcttgcatc aacgctgaaa tagagcaaat ttgtgaggtt
     5401 ttaacaaata cccccaaagg aaaattggca tttatagcgg attatgaggt aggtgcatcg
     5461 aatagtattt accttttaca taccattgcc gatcgactaa acgccgatat ttatgctgcc
     5521 ccctatcatg tgcgtgaagt aattaatccc ttaagtgtaa attacaaagg tagattgtct
     5581 gataaatctg gagaagtgca caacctgctg ctgcaatatg aaactgtggt acttttaggc
     5641 gaaaaagtga aagggtattt gtatactggt caatcttcta ttccacaggg tatacaattc
     5701 atacaaatca gtgccgcatc ggaacatctg gcctttgact atccttgcga tctggcaatt
     5761 cttggcaatc ttgaggcgac cttaactgct attagcaagc atttaaaaat taatggggta
     5821 agtgtgccgc ttaaaccagc caccaacatt aaaaatttgg aaaaaaaata taagcaacat
     5881 cctctgcatt cgttgattgt taccctcctg aatcaagtaa atcgctctgt tcatttaata
     5941 acagaaggat catcagaaga tgctgttgtg caggacattg caacaacact aggtttttta
     6001 aatgtgaatt tttcacccag agggggtgga cttggctggg ctatgccatt agcaataggt
     6061 atttctctag cgaccagaca ccattcaatt tgttttgtag gtgatggagg cagcatgtat
     6121 gcgattcatt caatatggtc agctgccaaa tataagattc ctgtcatttt catttgtttt
     6181 gttaatcgag aatacagagt attaaaaaca gatatgtctc aaaatagatc tttcatagaa
     6241 gaagattttt ttcgcctgga tttgaatcat ccacaaattg atgtccaaca aattgcctta
     6301 tctttcgggg cacaggtgaa ggcaattaaa tcaaaagaag aagtagagac tgttctcaag
     6361 agtagctttg cattccaagg cccaacattt attaccctat atcatcaata aaacatttcg
     6421 agagccagac attggctttt gcattatctg aaaatgcaaa gcataaaatc agtctcatta
     6481 tttaaaaatt tgttatttta caactcatag gaaaattatt gccgggaatt aatttttcag
     6541 gttagtcaga ttgcaatcag aacacaaacc tttgatttct accgtacaat taataattga
     6601 aaattgaatg gaattcgccg cttctgttac cgaagtaaaa tcaactacac attccaattc
     6661 tttgacaaaa tcacactgat tgcatataag aaattgggct tgtcccacat gatgcccatg
     6721 taaacaggcg acataggatt tcagactgtc aatgcaatga ataaatccct cttgatgcca
     6781 aaattgaata gcccggtaga ccgttggtgg cttggggtta tccacttcca tagataatct
     6841 ttgcagtatg tcataagcac cgagtggctt tctctcatcg actaatattt tgaggacacg
     6901 ctcccttggc tcggtaaatc gatagccatg gtttacacaa tgtttataag ccttatccaa
     6961 caaattatgt ttcattaatc ttctttcaag cgaatagtta gtgcgcacta ttttatcata
     7021 atccctgttg caaggaaaaa tgatcaaaca acagccaatc attatttcat agaaatcaat
     7081 tactgtctaa aaattattta atttttcttg ttatctaaca attaaatgtt acaatataac
     7141 attttttgag aaaggcagta tgtgcttgta acgcctggct taggaagaaa tcttattagg
     7201 gacaaacagc tggatctatt gtgcagatca agtttcaaaa taggcatttg cagaaaatta
     7261 acctttctct atagcaacca gaaacccctt taaatagagg aaagccatgc tgtatagtaa
     7321 ttatcgattc taccttgcaa aagatgccca acttctcaac ccggaagaat ccagagaagc
     7381 aattgagcag attttggcta aaactaaaga gttggagtta ataagagtag ctgtcgattt
     7441 gactagtcca aataaaagca tctgcttatt gagcgcatcc aaagagagtc atgtcataaa
     7501 aaccaaagtg acccagattt taaaggagca aggcctttct ataatagaca tgatggaagt
     7561 agaagtactc caggatattg aaaaaataga gcatcaccat cctactcata agtctaagga
     7621 cacacatctt catcccatta aaaagcaccc aaaaaaagcg aaacatgacc attctcatgg
     7681 gcatgggcac caccatggac atcatcatga aaatcattgg atcaaagcag cattaggttt
     7741 gatttgggga attggattac ttgttctttc tattgggact tttaatattc ccctggttgc
     7801 ttactatgtc attacaggac ttacctcttt gatgactttg tatttgggcc atacagttta
     7861 tcaatcagcc tggcaagcat tgaaagaaaa aaaatgggac accactacct tatactcaat
     7921 tagcactctc actattcttg ctgtttctat tgcgagcgtt tttgtccctg gcctacccat
     7981 gatgtttgag gcagcccctc tggttttagg cttttggcat ttaggtgaag gaattgaaca
     8041 tactctggtc ggtaaaatca ataaaaaatt ggatgtccgg gattgtgtac caccattagt
     8101 cctgttaaaa ggtaatccgg ataaagagat ttcagtcaag acattaattc ctaacgacat
     8161 gattatcgtt aaaaaaggac aggtcattcc tgttgatggt gtcttaacgc aaaaagcgct
     8221 cctttatacc acaagaattg atggctcgcc caaactcaaa gaatttcacc ctggcgatgt
     8281 tgtaaaagct ggcatgcaat tggcagatca tattccttct ctggaaatgc aggttaccaa
     8341 aacttatcaa aattcttatc tgtcattgat tgccgaaaac attaataaag cgaatgatga
     8401 gaaagcgcct atcgaaatgt ttgcaaacaa ggtattgaaa tattttatcc ctggtttgat
     8461 tgcggtagcg cttctatcgg ggattgtcat cggttcgttg tttaacccgg cactggctat
     8521 tcaatgcgtc gtgtctgttc tggtaagtgc ctgcccgtgc gctttgagtt taattacccc
     8581 aatggcagtt aaaatcggta tgaaaaaagc ctctgaaaac ggcatccact ttaacaacgg
     8641 taaagccttg caagcagcag ccgacattga taccgttgtt tttgatttaa atggcactct
     8701 gactcagggc aaaatggtgg tgaaatcatt gcatattacc gataaaaagt atttgcgccc
     8761 tattgcatta ctggaaagcc agtcggatca tccggtagcg aaaattatta aatcttacat
     8821 agaacagcaa gacacggttt caaccgaacc attagaaata gcctccattg acaaaagcca
     8881 tcattctggc atgaaaggta ttattaatgg tgaggccttt atgataggga ataaagacat
     8941 gcttgcagcc aatggaatta cctctatcga caaaccctat gataatcctg aaaaaggctc
     9001 tgtttatatt gtgcgcggga caacggtgat aggacaaatt gccttaattg atccattgcg
     9061 agaggatgcc attgctacag tgaagcaatt gaaacgtctt ggtaaatccg tacatatttg
     9121 caccggggca gatcaggcta ctgcagaaaa atatgcggca ttattaggca ttcccaaaga
     9181 taatatctgt gcgaacactg tgggggctgt cacgaaaaag ggggaagtat ccaaaaccag
     9241 ttatattcaa caattaaagc aaaaaggctt taaagtagct atggttggtg atgccgctaa
     9301 tgacgctacc gcaattgcct attccgatat aggcattgct gttaaatcaa gtatcggcga
     9361 ctccataacc gaacaacatg ccggcatagt cgtacaacaa gggttattat ttcctatagc
     9421 aacagcattt gatgtagccc aaaaaaccaa acaaaatatt ttccaaaacc tgttcgtgag
     9481 cctaagctat aactcaataa ttacgcttgt ggcagcgggg ttgtttgtgg cattaggttt
     9541 tgcactaaat cctgtgctgg gtgtcgcatt gatggtactt gaatccacta ttgttttgac
     9601 gaacttatac cttttcaagc aacaagacat tgtatcctca acatcaccca atgctaataa
     9661 tgctgtagag gtcgagccca ctgtcaatac aacctcaaaa gtcttgcatg ctttagggca
     9721 tcgttcccag cctcaagaaa atttagcaat tgtgacagag tctaaactag tcaatcccaa
     9781 agtaataaat ttgtttgcgc ctaaagaact ttcttctaat agacctgatg tcactcaaca
     9841 aaaattgggg tgtgtaacct gttgatcaag taatactgaa ttgccttgat tttctacttc
     9901 aaggcagttg ttacgataat catcttttcg ttaataactg ttttttggac tatactcata
     9961 tcaagacaat gcaaagcatc acgccataaa attaggatga ttcgatgcca tgaataacac
    10021 ttggcatcaa accgccactg gaaaccgctc catttcaaat tatttgcgat taagcagaac
    10081 tctccttaac gtcatcttta ttattgccct ttccctgctt ttaatcatca atatcatctc
    10141 ttataaccag gtcaaaaatt tactcttggc cattaactgg gtttctcaaa gctatgaaat
    10201 catccaaacc attgatactt gtctttacga agtagtcagc atcgaatctc gccagcgatt
    10261 ttatctgatt cgagactcaa acaatcaaca tatggtcgat gtggatgaaa tgaaatccgc
    10321 tttaaaactg aatttagaca aactcgagaa attaaccagg aataatctgg aacaaaacaa
    10381 gcgtaccaat cgttttactg atttaattga gcagagatta aatttgttaa atcaaatagt
    10441 gcagttaaag gccattggca aattcaatac gcatgaaaat tatgatttac tgaatcaaag
    10501 tcaagacatg tctaaccagg ttaaaagtct tgggcaagaa atcaaatccg ttgaattggt
    10561 gctgttatca gaaagaaagg ccagtgctgt tcgccgggct gatagaagta gctttacttt
    10621 aatatcaggg ggcataacaa gcatcttgtt tttattgatc gcttttattc tggcaaatag
    10681 tgaattgtca acgcgcgaaa aaaccgaatt gcagaatcag aatacgcaaa cccaattaaa
    10741 gaaagtcata gaaagcgcca gtgatatgat cgcagctttt gacaaagaac accgatttat
    10801 tacttttaat gaggcatatc agcgtgagtt taagcgtctt tttgataaat acataagcat
    10861 caatctgtcc ctggagaaag cgctcgttga tattcctgaa aataaaaaag agttagctga
    10921 aatatggaaa aattctttac aaaacaatga agaaaataaa actattgaat tgaataccga
    10981 acatgaaaaa attgtttatg aaatgagctc aaaactcatt caaaatggtg ataatgaaat
    11041 taatggcgtg gtgcacagcg ttcgcaatat taccaaacgg gtacaagaac atacggaatt
    11101 acaagaatca tatcaaaaac tggctaatgg aatgaaagag ctgcaagaga aaaatgaaca
    11161 aatcacctta ctggttgaaa tgagtgacat tatgttggct tgcagttccc agcaagaact
    11221 cagtgacgtt ctgtccaaat actcccaaag actgctgcga ttttccagtg gatatttgtt
    11281 catcatgcat ccgtccaaaa actatttgga aaaaaccacc agttgggggg atccccaacc
    11341 acacgaactg acatttactc ccgagcaatg ctgggctatc cggcgcggtc gagttcatca
    11401 tgtagactcg tctcatattg agttattatg tggacatgtt tattttccct cgaacagcaa
    11461 gatttatctc tactttgcgt tcctttaatg gcccaaaatg atatttatgg cttgttatac
    11521 ctggaagtca atctaaagca actcccatta tttgatgaga atcaacggtt gttaataact
    11581 gcatttgctg agcttaccgc attggcactg gcaaatgtcc gcctgggaga gagtttacgc
    11641 tatcaatcta tccgcgatcc actcactgga ttatacaatc gccgttattt ggaagatttt
    11701 ctatttaaac agctacatca agctgaacgg acaaaagctt cctttgccat tttaatgctg
    11761 gatttggatc attttaaaaa aatcaatgat acttttggcc atgaggcagg agactttgta
    11821 ttaaaggaga taggccaaat tttgaataat gatattcggc tgggtgatgt tgcaactcga
    11881 tatgggggtg aagaattcgt cctgttactc tttgacgttg atgccaacgc cgctaaaacg
    11941 agagccgata atttgcgctc agccatttcc aaattacagg ttaaatacgg ggcacaatat
    12001 gttggacagg taaccgcatc aatcgggatc tcgatatacc ctgacgatgc caaatccccc
    12061 gatgagctga ttgaagccgc ggataaggct ttataccaag ccaaaaacaa aggcaggaat
    12121 atggtgcttt tattctccga aatatcatcc taatttggat agtagctcct gatttaaatg
    12181 ttgtggtgtt tcttatgtcc gatagaaagc tccatgtgtt atacgagttt tgagagcgaa
    12241 acaatagata ctatactcta ttgcatccgg tatttttagt agcaaccccg tctacgataa
    12301 tatataatgc gcagccataa ggcataatct ctcctattca tgctctatca aggaattatc
    12361 tcgaatgttt agcaaagccc taatcagtgc aacctataaa aatggaaaac ccggccaact
    12421 tacagaagaa catgaaaaag cggagcatca ccgctttaag acagaatttc tgtttaaaaa
    12481 tgcagagatc tctaaagagt tatatgcttc acgcttgatt cagcattttt ttattattaa
    12541 agccatagaa acaaaattac aaagtttgtc aaaaacagaa cagtcagaaa tcagcgcatt
    12601 ttttgctata tcttatcttg agcacttatg gcgtagtcct gctatagaaa atgatttaag
    12661 acaactaaac atcaatcctg atgagattga taaggatgag ataacaaaaa caaccgaaaa
    12721 ttaccttgaa aacataggga aatttacacc caaaatcctt ctcgctcatt ttttattaca
    12781 tattgccggg tttatgcatg gcgggaatat tattcgctca aaatacattg aaccaagcaa
    12841 tcgtttgacc tcctaccaaa tagcagcaga tcaatatgat ttctcttctg ccatctcctt
    12901 tttatcctcg ggaaggcatt cgccactcgc tctttatcag gatatgatga aacaaatcga
    12961 taacattgct ctaactgatg gtgagtatga tgagattctt gagcaatgca aaggcatcta
    13021 tgaaaccatg agcaatatct acgatgactt atgtgacatg catgctcatc acctgaagct
    13081 gcccacatac tcacttgcgg ctatcagcgt cagtctggtt gcgcttggat taattctaaa
    13141 actattgatg gatttcttaa atccagtgac gaatcctata gcaaacgccc ctcgttaagg
    13201 cccatcgtca gaatgttatt gtggcctgga tattaagatt tacaatgatt tatttcttta
    13261 tagataatca ttacccactt ttatagaaat ttgaacaata tcgaacagat agtgttgttt
    13321 tgataaacaa atgaaacaat aacagatgtt aaaacgggat atttcaaccc tgaatacatt
    13381 agttgcatca gctggtggaa tgattggtaa tgttacagaa ttgccagtat acaattcttg
    13441 aggtagcaaa gcgacaaaat aaaaatgcct cctgattgtc cttgtctgtt ttgtagtgaa
    13501 gtgtcataac ttcttatggg taaattgcat caagggtaac ttgcctgctt cctgccaagg
    13561 taccctgatg catattgagt ttgcaacatt tttttacgat aaagcgatcg catcagtata
    13621 agattcgtct aagttccgct tcctgtaatc gagctgccaa agactcatcg gactccaaac
    13681 cggtttgcac tttgccagaa acttgagtat tccttttatt ctgctcataa gtaaagaaac
    13741 cataaggcga taaggtatca aacagcgttt caaaatgttg gcggtaacgt tttgctctct
    13801 cggattgagt ttgggttctg aaggtcctgt caagagtttg ctcttctttt aaaggaacgg
    13861 ctctgtcaga tacagtttca taaccaaaag ctcgcttacg gggtgtgctg tattgtctgg
    13921 gtccctggag ggattctact tgtgttatcc aatgtgtatt actcaggttc ttaagagtaa
    13981 ccgtaggata ttccggaaga ggtttgccat tcggttgtcc aacaacgttg aggttgattt
    14041 taagtaggtc ggcaatttct ttcaaatcat catgtgttgc ccatcgtcct tgttctttaa
    14101 ttttttcaag gctcttcaag ataattgaat ttgaattgat tgtattgcct gatagaacat
    14161 cgtttagtaa gacttcctta acaaaagcat tttctatttt ctgtacctga tgaaaaggct
    14221 ggtttctgag gattggtccc agtattctgg caatatcttg cgctgtttga acaatttccg
    14281 gagagagggc tagttcattg aattgactta tccttgccag cgagtctctt cgatagagat
    14341 aaagttgtac caattccatg aatttgcaat aagcatttga tccttctatt ttggtttgat
    14401 ctttacttcg accttcttcg attattctct taatcaaatc ttctttattc acaatgactg
    14461 caatatttcg taaagacatc tgcaatagaa ataataattc acttttataa gtgtagggtg
    14521 aatcatgaag ttttgttaga tcgacattaa ggatgtcttg cagtcgtacg ccataaatac
    14581 cttctacttg cttccatcga tcataagttt tactttttcc gtgaagtttg tattcatttt
    14641 gtatgatgct tattaaacca atagcaagag cataaaatcc acaatccccg ccaccaggat
    14701 tatctattga atatgacatc tgtacactcc cttttaggat taatgattta atgtttcagt
    14761 caagcattat aaaatatgaa cattaagtaa atattatgta acatatgcca agggggagtg
    14821 caagtagcta acaattatct tttgcctcac ctgtgggtaa gcaagctatt tatcaattgg
    14881 gtctccaatt gatattcgtt attattgcgt ggaagaaaaa aatctcgcta aagccgtagc
    14941 ctcttgtaaa ttgacttctg cattaatcca atttaacttg acttgcgctg ttgtttgttc
    15001 acgttgattc actaaaaaaa gcgtgctatc accttcataa aatctcttgg tttctccctt
    15061 ctctacttgc cgtgcaagat ttaactcttt attaagcaaa accacttgct gctgatatat
    15121 tcttattgca accaagagat tggacagctc gttatttaac tgttcgtagg taaacttcat
    15181 ttccgttttt acttgctgta attcgctcgt ggtgctgata agctttcctc ttgcttcacg
    15241 ttgaaaaata ggaaatttaa aattcacgcc gatcatagca gcctgaggga tcaataaagg
    15301 ataaccgcct gttccatatt gtttaaaggt ataagctgtc gcatcaagat taggaagaag
    15361 ctcatttctt gctaaatttt gttttaattt aacaattttt gaataattct ccaatttacg
    15421 caaaacagga tgccgttgta actgcgaaat actattggat actcgaattg gttgggcagg
    15481 aaatgttgcc ggtaattgac tttcatccgg tttttgcggt ttgccattcg cgtcacgata
    15541 gtaaagtgaa agattgatgc cagcctgcgc caataacatt tctccttgat tgaccagttg
    15601 ttcacgttga acaataagtt gtaaattttc tgcaatggca agtctaggca aatcaccttg
    15661 ttgagcttgt tgaataatcg cattttgccg ctctttcgct aaatttagca aatgcctgaa
    15721 agttcttaac tggaaacccg cctccaccca ttgccaatag gctttaatgg tttcctgata
    15781 aattttaatt tttgtggcag cagcatcctg ctgtttcatg cgaattgttt cttctcttgt
    15841 aaaaagttcg gtacgctctt tatcaattcg cctgtctcta agcaaaggta aagaaaggcc
    15901 cgcacgatac tcaccaccag agttagtcag gtaattttga tagtaaatgg gccaatcgcc
    15961 tctgccaata cgatagcctc caaacaattt aagtccatta tagagggttg gcacattaaa
    16021 aagattatct gcgtaattat taatataccc cccgaccggc tggaagcggg gattcacatc
    16081 gaaagagggg tcaaattttc ctaatgcatt gacataatca ccttccgctt ttctgatttc
    16141 aagacgcgct atgactatct gcgggtaact tcgatcaaca cttttaagga cggcatcaag
    16201 cagcaaaggt ttggctggta ttggaaaaca cttgaatgac agcatcaaga acaaacataa
    16261 aaccatcaat ctgtttttgc ctgaaggaaa acaaatcatg atgctttatt ctcgcgatta
    16321 ctttccggtg ggaaaccatt atactgacgc caaagctcat accataaagg aacttgaccc
    16381 aattggatcc acccatgcac ccgcactcct tgccgtaaaa aacgcacact aggccatggc
    16441 tcattaggaa caatcactac acggaataag ccaaagccat tatccgtagg atcaataaag
    16501 gagactgtac ctccaaatgt tccgactgca atttctggcc atccgcgaaa ttgaatagca
    16561 ggccatcctt caaattgcaa acgggctttt tgattaaggc gtacgaaagg tatatcattg
    16621 ccatcaaccc ataattcaac agcgcgagac tctgtttcag gcagaatttg agccaataca
    16681 tcgcctgcgt tgacaacgac actttcctga cctgtcaaac gtctaaagat gatacctgcg
    16741 acatgggcct taatgatttg tgttttttga cgagcaattc ggacatcgac attcactttg
    16801 tcaatattcg cctgcgcaag gtcattttgg tattttgcat attcaatttg tgccaattca
    16861 tattgccttc tcgagttaat tccctgttca tacaatttct tttgtctgtc aacattggct
    16921 ttacctgcag caatagcttg tttggctgct tctatacgaa gcaaaatcgc ctttttctcg
    16981 acttcaagac gtgacaaaag ctccggatca ttgtcggtaa tatcgactat tggatctcct
    17041 ggtttaacac gcataccttc gtcgacatac catttcttta ttcgcccgct aacaggtgaa
    17101 ttaaccgtat gctgcctctc tgttggtgaa aaggcgatga cttttccgtt gcctaatgca
    17161 aactgttgcc acggagtaaa acttaagaaa agaataatgg caacaaaaac aatgaaaatt
    17221 attttcgcta ttttttgagg cttgggaagt ttactaatca tagaataagc atgtaatttc
    17281 atgaggaaat caccaggcgg ttatttaaat agttaaaatc ggaccgttgg ctcacgatca
    17341 gtagtagcgt attttttaaa gacaatacca attccatcat ctcattcact tcttgatgat
    17401 taaggatgtc aaaagcgcga tcaataacaa ttaattcagg ttgtaataga agagccctga
    17461 ccaacatcaa tttggtgagc tcaactgaag taaacacatc ctgccattca tagacaatgg
    17521 tgtgtagacc ttccggcagt tgtatgattt tatcagcaag tccaacattc tgtaattgtt
    17581 caataatgat tttgttagac atattacgat gattaagtac taaattgtca taaatgcttc
    17641 cggtaaacca ctggggttca gcaatcaata aactaaaacg acgaagagaa atgagggttc
    17701 ttttcgcaca aggaatctcg ttaataagca catgaagtga cacaggatct tggaacccaa
    17761 atagttgctc aacaaacaat tggcataaat ctgatttttc cgaaaacacc aataaaggct
    17821 tatcaactgc tatcaatgcc ttattcttgc tatccatctc tagctggata ctattaattg
    17881 gagtcaataa ctcaccgtgc tcatcttctt tcacctgttc aagtgacagg ttaagtactg
    17941 catcgatttt attttctgag gcgaccagat cataataatt ttctaaaagg gcaccaaagc
    18001 gtctaaatga gtaaatcaga gcacctaaaa cgatttctgc ggcaaccaat tggcccagac
    18061 ttaattgatt attaataaca agatagcccc ccaatcccaa taaaaggctg ctggccaaag
    18121 cagacagcat gtaaaaaccg atttgatgct tgataagttg cttaaaatgg gtgtttcttg
    18181 cttttaagaa agcaaccaat tttttatccg tttgttgcgt cacataacgg tggtgtaaat
    18241 taaaacggaa caggtaccgg ttaatcaaaa tttcctctaa ccaagcgcct acgacgtgtt
    18301 tttcagagca ttctttttta gcgctgtcta atgctttttt ataggggata aaaatgatca
    18361 atagaatgcc aataatgatg aaaatatcaa acaaaaggaa aagagggtga taaattagaa
    18421 tcagcaatag tccaaaaaaa agttgtaacc ccaaattaat gccatataac aataaacttg
    18481 ccagtgattt tttgatagtg actatttcaa aaaatcgatt gaccaactcc ggtccatgat
    18541 gtgtcgaaaa attatcaagg gacatgtggg taaaatgtcg tgtcagactt aaactgactt
    18601 taaccatcag tttttgctga attacctcaa taattacaat ttgccatata tggagagcac
    18661 ctagagccat catcaaaacc aataccatca aacttaatgt aatgacgggt tgtaatagct
    18721 tgccaaaagc aattaaattc acaagtgttt gggcagcaat ggggatactc aatgacagta
    18781 agctgataat cacactaatc atcgcgatgg atgagattgt ttggcgatct aatacatcaa
    18841 taattgaatt aattggtttt gcattcattc cctaaacatc ccttttaccc ttgattcttg
    18901 tttatgctat taatgtttta atttttgcaa tataaaaaac tcaagcgatt caattgcttt
    18961 attgctgtca ctctttttta ataaagagac atggacatcg ttcaaaacag ggagaaaatc
    19021 gtaatcgagc cgttccagat agtttgggat catgctgcgc tgaatagctg taattcctaa
    19081 cccggcgcgc acagctgcca tttttcccgc ataacttgcg ctactgtaaa caaggcgcca
    19141 atcaatatgg tttttatcca gtgtatcaat cacattgcct cgataaacgc agggtgttgg
    19201 tgaaagcact aagggaattg gccccggttt gtctaaaaga ggtaataatt cttttttccc
    19261 tatccactca acgggttctg tacaaacatg gatagactca gaaccaacct gttgctgagt
    19321 cgttttaatg agaattaagt caaatttacc ttcattaaaa cgctcgatca gattcagagt
    19381 cagatcacat tctacattca ataagactcg tggatgcaat cttgaaaatt caacaagtac
    19441 gtccgataaa ataatactgg caaaatcctc tggtaatccg aagcggattt caccttgtaa
    19501 ctcaggcgct ttaaaacgat ctaaagattc tcgatgcaat tcataaattc gtttggcata
    19561 ccctaaaaat atttcaccat ccgtggtcag agaaagttct cggccacgat taattagagg
    19621 tttttcaatc aggttttcca atttagctat ttgctgactg atagccgatt gggtgcgtcc
    19681 aacccgctgt gccgccttgg taaaactttg cgtttcagcc actgccagaa agcattgtag
    19741 tgtgattgta tcaattgaca taagtttttc taatagttta aatcattatt tttaatttta
    19801 cttatattaa catatctaat atatttccac aataataaca gattttctaa tggggaatta
    19861 atgtggataa acttttaaat gggttatcga tttcactcat gctatcaccc ttgatagcga
    19921 tgctattcta tagcttgtta ggaaaaacaa gaaatattca agcaacttat tacggtacca
    19981 cagttatagg ttttggtttt ggattgagtc tcttggcatt aattgtttcc gcaaatactg
    20041 taaaccaaaa cagttatttt ttatttcatg ccagcacact aagcctctta ctaagcacat
    20101 taatattatt catcagcttt attatccacc gtttttccct acgatacatg catggcgaca
    20161 agtgctaccg acgttatttt ttcaaacttt cagccataac tttaacaact gttgtgatgg
    20221 ctttagcaga caatctttta ttgttctggc ttgcctggtc agctagtaac ttgctcttga
    20281 taaacctcat gattcataag tcacaatgga aagcagccta caactcaggg atactgactt
    20341 taaaaacatt attaccagga agcttgttgc ttatcactgc attttggatt ctttttacat
    20401 taaacaatac tctgtcaatt gaaacaataa ccagcgaagt gagcaataag tcatcccctg
    20461 ctattgctgt ggcacttgga tttattatct taacagcgat gacacaatcc gccatatggc
    20521 cttttcatcg ctggctaata agctcattaa actctccaac accggtatcc gctctaatgc
    20581 atgctgggtt agttaatgga gggggctttc tgattgttaa atttgctcct ttgtttgtca
    20641 tgcaaacaaa tttattaacc attttattca ttttcggtgc actaactgct ttattaggta
    20701 ctctctggaa actattgcag tggaatatca aaagtatgct tgccagttcc accatggcac
    20761 aaatgggttt tatgataatg caatgtgggt taggactttt cccggccgct atcgcccatc
    20821 tatgctggca tggcttgttt aaatcctatc tttttctcaa ttcaggatct gctgtccaac
    20881 aaaagaaata tcaaaataaa tcacgtcccg aaaaattgac aactttattg actgcatttt
    20941 ttggaggact tttgggaatg tatggttttg cccttatcac aggcaaaccc atcttatctc
    21001 tccatgcgtc cacctttctt ttaggtttcg cattcatcac agggacacaa ttcactctgg
    21061 gcttgattgc atatgagaag catttcaaaa aggtaatttt agccttggct ttagttttct
    21121 caattgggat tttttatggg ggaagtatac atctgatcga gtcactctta cctgatctaa
    21181 caggatttcc tcaacaccaa ttaagcagct tacaagtctt aactctgatt gtcttctttt
    21241 ccctttggct tgtattcagc atgggattat tcaaaaaaat cagagagacc agactttggg
    21301 attggtttta catgtacatg tttaacgcaa gtacgcctca ccccaaaacc attacggcaa
    21361 atcgccatac ttattactat taagaaaagg agccaacatg agtagccaca tcacactaat
    21421 caaaactaac acgaacaatg aaaatcaaat gacccaattt aactacaaag tcgataatga
    21481 tgtcatgatt atccgggcta tggtaaataa tgttgcaaag caaattacgc ctgtatggcc
    21541 tttggaaaaa tttattgcct gcaattcctt gcatggtttt gaatccatgt cctttgaaga
    21601 ggctgtgata caaaaccaaa cggccaaaaa aggcactcca ttcaatgaaa aactggaaag
    21661 agtcaattgg cacatgatca aatggtgtgg cagctttcta gacattggac aaggcacgct
    21721 tgaaatgcct catcgtgaca aaggcttata ttttggtttt ttaaagctag ccccttttga
    21781 cagcgaactg catcaaaaca ataaatcaac aaagagctgg ttatcaaacc tgccagaaat
    21841 gcctgaacaa gctatccgac tttgcctcaa caaacttggt gtattaaacc aaaaacaaga
    21901 gaaatttctc aaatccactc tgtctcactt acctggatgg gctggataca ttaagtggat
    21961 aagcgaatgg aaaaatcgca atggcaagga agagaatcct gttagccttg ccgattttct
    22021 tgcagtgagg ctgataatta cctgcgtatt atggccagaa gcaagccagg aagaaaaaaa
    22081 aaagaaaaaa gataatgcgt atactaaaca acttattcaa aatataaaaa ataaagaaga
    22141 cgattacagg caattgcttt taaacaagct tttgcctgaa ttagacaaag ttcagataaa
    22201 agaaaacaga gctaatgccc aaatggtttt ttgtattgat gtacgctcag aaccatttcg
    22261 gcgttgtata gaaaaactgg ggcactatga gacgttggga tttgcaggat tctttggttt
    22321 acctgtgagc atcaaggatt atgacggtga aacaataaaa gactcctgcc cagtcctatt
    22381 aaagccacga tttaatatcc atgaaaaagc aattgctgca aatgaacact gcatagagca
    22441 tcatgagaaa ggtaaagaat tcaaaaatat tttgaaccgg atctatcagc aattaaaata
    22501 taatttttca accccatttg ccctggtaga atcattagga atttggtgtg ggattactat
    22561 gttccttaaa tcatgtagcc caattttcgc tagacgcctt accaaagacc taaatgaaat
    22621 gatatgcccc tccattcaaa cgcagcctgt ttttgaattg gatttattag agaaagaggt
    22681 agggatctca cttcaagaac aaattgccta tgctgaaatg gcattgaggc ttatgggact
    22741 aactgataac tttgccaaac ttgttatttt ctgcggtcat ggcagctcta ctcaaaataa
    22801 cccttatgct tcggccctgg attgcggcgc ttgtggaggt aatcaaggtg gaaaaaatgc
    22861 gcaattactg gcgtctatcc tcaataaaat aatagtgaga agggctctgg cagaaaatgg
    22921 aatcaatatt cctcaagata ccctgtttta tggcgcgcaa catgacacga cgacagatga
    22981 ggttgaaatt tatcactcaa atgtcagtca attcattcac caagatatcc tggaccagct
    23041 tagaactgac ttaaacatag ctaagcacaa taacaattta gaacgaatta actatttgaa
    23101 ttccatagat tgcgcggaga aagacattgc aagaagaagc acagattggt ctgaaacaag
    23161 gccagaatgg gggcttgctc gcaatgccgc tttcatcgta gcacctcgcc aactgaccaa
    23221 aaacattaac ctggaagggc gttgtttctt acattcttat gactggtccc aagacaaaga
    23281 cggcgccctg cttgaaacca ttttgactgc acccatggtg gttgcccaat ggattaatac
    23341 tcaatacctg ttttcaacaa tcgacaacgt ggcatatgga agtggcagta aaattactca
    23401 taatgtcgca ggaaaaattg gagtcatgca aggcaatgcc agtgatttaa tgcatggatt
    23461 gccactgcaa tctgtcatga gccatgatga caaatccttt catgagccac aacgcctgtt
    23521 aactctagtc tatgctcctc gcgaaatcat cagtgaacta gttgaaaaac acgatgtttt
    23581 aaaaaccttg ttttttaatg aatgggttca tttggtagcg attgatccgc ggagtcactt
    23641 gttttacaag ttagagaaaa ccaatacctg gtctgtaatt cagtgaaagg agtctcctga
    23701 tgaatataat caaagtatca gaaaaaaaag agatgaacat ctctttgcat ccgatgaaaa
    23761 agatcgaagt gatcgtatct ggtgacaatg aaaatatggt cgctaccatg atggagaagg
    23821 caaatatttc tggttttacc ttattgcgaa acgtctctgg aaaaggacat catgggtttc
    23881 atgaaggtaa agttttattt aacgacaaag cctctttcgt catgtttatt gcagtggcat
    23941 ctgaggaagc tattgctaat attgcgcttg gcatgaaaac tctattccaa aagaactcag
    24001 gagtcctgtt tgtgtcggat gtttctgttg ctagagtcga ttatttctct tagtatttaa
    24061 ctttggaggt gacccctatg cactatcaaa atcagcgtgt tttactggcc tcaaaacatc
    24121 aaaaggaaaa agcgattcag ccaccttttg aggaaaaatt aggctgtcgt atccatgtgc
    24181 ctgctgatta tgatactgat cagttcggca cattcaccgg tgaaatacca agaaaagcct
    24241 cagcctatga aacattaatt ctgaaagcta aagcagcagc caagcaattt ggataccgtt
    24301 atgtaatatc cagcgaaggg agtttcggcc cccatcccca gctttatttt tgtcccgcag
    24361 acaccgaatt gatggccttt gttgacttgg ataatgattt aattatcgcg gagcacgaag
    24421 tatctacaga gacaaattac agtcatattg atatcagtgc cacagacgac tatgaagcgt
    24481 ttctcaacaa agcaaaattt ccaagtcacg gcattattgt tcgagcctta aatagcgaag
    24541 aaaactatat tcaaaaagga atctgtgagc gtgagcaact taaaacagcg attcaaaaag
    24601 cgttcacata cagtacaact atccgccttg aaaccgatat gagagcgatg atgaatccgc
    24661 tgcggatgaa tgtaattaac aaacttgcgg tgaaactggt tcaccgcatt cagcaatatt
    24721 gccctcaatg ccacactccg gggtttggaa aaatctcgtc tgaaggtcat ttagcctgca
    24781 tttcttgtgg aacaccaaca gaactttacc tgcgaaaagt tttaaattgt ctaaaatgtg
    24841 aatttaagac ttatcaagcg cgagacgatg gactagaatt tgctgaccaa aggtattgtc
    24901 cttattgtaa tccttaaccg tttgcgagaa tcaagtccat ggtatgttat aaacattcaa
    24961 acaagagagg cacactatga aacccatatt aataggtcta attatggggt ctcaaagtga
    25021 ttggcaaaca ttaatccatg ccgcccaaac actggatgcg ctcaacattg gctatgaggc
    25081 agaaattgtc tccgctcata ggacacccga caaacttttc cagtatgcgg aacaggctga
    25141 ggaaagaggt ttggaagtga ttattgctgg tgcaggagga gcggctcatc tcccaggcat
    25201 ggtagcagcc aaaacatctt tgcctgtttt aggtgttccc gtcatgtcac aaactttaaa
    25261 cggcgtcgat tccttgctat ccattgtgca aatgcccgcg gggatacctg taggcacttt
    25321 atcgataggt aaagcaggtg ccatcaactc cgcactgttt gcagccgcaa ttcttgctaa
    25381 taaatatcct gacattcgag ctgccttaaa acgttatcga gaacaacaaa cacaaaaagt
    25441 gctcgacaat ccaaacccta aagaggaaaa gtcatgaaac ttggtattct aggtgggggc
    25501 caacttgcaa gaatgttggc attagcagca tatcctttag ggattaacac tgtctgcctg
    25561 gatcccagtc ctgacgcctg tgctggtcaa ctcaccaaac tgatagtctc tgattttaat
    25621 gatgaattgg ctttaaaaca atttttgtca gaagtggatt gcgtcacaat agaaaccgaa
    25681 aatatacctt tttcttgcgc cgaattggct gcccaaataa agcctttcta tccatcaacc
    25741 caagctttga aaattacgca agacagattg tatgaaaaaa attttttcag aactctgaat
    25801 attcctgtac cagaattttt accggttgaa tcagaagatg aattgatgca ggctatttca
    25861 gttataggaa tgcctgctgt gttaaaaaca agaaggtttg gctatgatgg caagggccaa
    25921 tatgtcttgc gaacccaagc tgatatttca aaaagttgga gcctgttagg cagccaagct
    25981 ctcattcttg aaaaattaat ctcctttgaa agcgaatttt cgttaattgc tgttcgtaat
    26041 aagagtggtg aaacaagttt ctacccattg attaaaaatc atcacaggaa gggcatttta
    26101 catttctcag aagcccctgt tcatcaggat accttacaac aggaggcgca aactcatgca
    26161 ttcaaaattt tagaagccct gaattatgtc ggtgtactta ccattgagtt tttttatcaa
    26221 ggcaaccaat tgattgcgaa tgagatagcg ccacgcgtac ataattctgg ccactggact
    26281 attgagggag cacataccag ccaattcgaa aatcatctgc gcgctctttt tgatttgccc
    26341 ttgggaagca ctgaagtgag cggctcttgt tttttattaa attgcattgg caatctgctg
    26401 ccattggagc cctgtttatc gatcccgggc gttcattatc acgcctatgg aaaagcaccc
    26461 cggccagatc gcaaggtagg ccacgttacc cttgtcgata ctgatttgga acgttataca
    26521 aaaagtaaag acttacttct tgacatttca tgagtagcag atagtgagaa agcaagcaat
    26581 ggcagtcagt gcataatatt gttcgcaatt tggaaagctg agttttgtat tctgtttcct
    26641 actgctcatc tacattatcc tgcttattat tggttgtcac tatagactca ggatcaatgg
    26701 agcttccagg aatttttcgg ctttcgctgg cccattctcc caaatcgatt aacttgcatc
    26761 gctcagagca aaaaggcctg aactggttat cagggcgcca ggtattttgc ttgccgcaaa
    26821 tggggcattt gatttttttc tggttgttca ttacggtttt gctaatttat ataatttaga
    26881 tatgaagtat ggctacagca gcaatgattt tcaatacacc tgcctctttt ggacaatagg
    26941 aaaataaatc tcttgcattc cccaatctaa aactaattat caacatgcac ttcgcgcacc
    27001 agagccaacc aggctttcgc agcctcagag agataagaat ttctacgcca agtcatcgcc
    27061 atattccatt ggaactcact tccattaagt aaaagagagc gaatttgtgt cgtatgtctt
    27121 tgagtggcaa tcatccgtgg caaaaaagta accccaaggc ctgctgcaac aagctccagc
    27181 ataaaatcaa tctgactgct ttgagccacc accttaggtt caaaaccagc gtgacggcac
    27241 gcgtccaata taattcgatg tagtgcgaaa ccactgccaa aaaggatgaa aggtatttct
    27301 ttaagttctg gtagagaaat gcaatgttta gttgctaatg gatgtccaga tgccaggagc
    27361 gcaaccaacg gttcagagcg tactaattgg caatcaaatt cttcggaaat aggtaataag
    27421 gttccagcaa aatctatctc tcctgcacgc aaacactctg caagtttatc gctgccatgc
    27481 tcaacaagct ctacttcaat gcctggataa cgttgtctgt acagaacaaa aagaggggca
    27541 aataaagtac tgctgccgac cggagaaata cccaaacgca gtcgaccctg tttcaaacct
    27601 cggatttcat ccaattcttt aagaagatca tctcgatcag ccagtagttt ggttcctcgc
    27661 tgataaacaa tttcacctgc tgttgttggt acattacgct ttttaaaccg ctcaatcaga
    27721 gtgataccta actcctcttc aagctgtttt atggctttac tcaccgtaga ctgggtcgca
    27781 aatagatttt cagctgcttt ggaaaaacta ccctgccgta caacctctat gaaagtctga
    27841 agcagtttaa attccataat tattccatat tggaaaggtt attagtctat aaattcattt
    27901 tattcatctt aattaaaagt gtaaacttaa atgaaaaaat aatatccacc atgagcacat
    27961 atatggcaaa caaaattcag caaaattcat tttttcagat aatattggtt ggcttatttt
    28021 ggttagctag tgagttattg ctcaaactgg tcaagcttcc tttgtccgga ggaatccttg
    28081 gattgggaat tgttttactg cttttggcaa cgaattgctt aaaattaaat cgtataagac
    28141 gaggggctga gttactcctt gccaatatgt tacttttttt tatccctggc gtccttgcaa
    28201 tactggaaca ccaggaattt atcggtctat taggccttaa aattctcttc attattctgc
    28261 tcagcacaat cacggtgatg ctagtcactg cccttgttgt agattattgc taccgttgga
    28321 gaacgcatca tgccaagcag cattctctct gaatccatga tccaggcatt tttctggtcg
    28381 cttatcacaa taggcatgta ttttattacc caaaagctat atagaagatg gcccttttgg
    28441 tggctaatgc cgcttgcttt aacaccaata ctggttgcca gcatagtgct tttattgcat
    28501 gtaaactacc aggactattt ccgcggaaca aaatggctag tcttgttaat cggtcctgca
    28561 accgtggcct ttgccattcc catttatgaa aaacgtgccc tcattcgcca gcattggcca
    28621 gtacttttaa ttggcgtgat tgtgggaagt ttcactgcca tagcatccag ttggggattg
    28681 gcaatccttc ttggccttga tgataattta cgtttaagtc tgctcccccg ctctatcagc
    28741 acaccgtttg ctatagaaat ctcaagggga attggtgcag cacctgattt gactgcgatt
    28801 tttgttattg tcacagggat attgggagct atcctcggtg agttcatcct gtcgcgcttg
    28861 ccttttaact cagctcttgc cagaggagcc cttttagggg taggagccca tgcggcagga
    28921 accgctcagg cccataaaat agggaatacg gaaggcgcga ttgccagttt ggctatgatt
    28981 ttagtaggtc tcttcaatat acttcttctc ccccttatca gctatctttt gaaataaaag
    29041 tgcgttcagt agaagttaaa aggaactcct ctagttaaga aaaaatcata agcatgttat
    29101 tttatggttt ttccattaat agaagaaaag aatgactatt gctcttgtgt ggttccgtca
    29161 agatttaaga ttgaccgata accctgcgtt catcgaagca tgctcccatc atgaaactat
    29221 aattccactt tatatttatg atgataaatg cagtgtttta gggcaagctc aagcctggtg
    29281 gctccaccat agtctaattg ctttaggcaa atcgttaaat caacagggtt taagcctcgt
    29341 tctccgcaaa ggatctccgc tagagattat tttggaatta gtcacacagt atggtttgga
    29401 atcagtctat tggaatcgct gctatgaacc acaggtcatt gcacgcgata aaaaaattaa
    29461 gctcgcttta ggcgaaaaag gaataaaggt tcatagtttc aatggaaatt tacttcatga
    29521 gccgtggaca attaaaaata aaaatgggga tttttttaag gtttttactc cttactggaa
    29581 acaatgtcga caaattctta atatcccctc gccttttatg ctcactaaac gcccaagggg
    29641 aatagaaatt acaagtgatg accttggcaa atggaagtta ttgccagcaa ttaattgggc
    29701 agcacgattt tctgaatatt ggactcctgg agaagaaggc gcacaaaaaa aactgaatga
    29761 gtttattatt caccacctta gcggatataa aagagatcgc gattttccag caaaaaatgc
    29821 gacatctcga ttatcacctc atttgcactt tggtgaaatc agcccttggg tgattttaag
    29881 agcgctggag ttgactaaac tggaacaaac ctgtgatctg gcatccgtag agcatttttt
    29941 atcagagctg ggatggagag agttttctgt ctatctgctc taccacttcc ccaagctgga
    30001 ttgcgaaaat tttagaaatg aatttgatgc attcccctgg caaaatgatg agcaattatt
    30061 aacccgttgg caaaagggaa tgactgggta tcctctcatt gatgctggaa tgagagaatt
    30121 atgggccaca ggatacatgc acaaccgcgt tcgtatgatt gtcgcatcat ttcttaccaa
    30181 agatttactg attgattggc gtttgggtgc acagtggttt ttagacaccc tagtcgatgc
    30241 ggatctggcg aataacagcg cgagctggca atgggtagca ggatgtggtg cggatgcagc
    30301 accttatttc cgtattttca atcctgtttt acaaagccaa aaatttgacc cggatggcgt
    30361 ttacatacgc caatggatcc ccgaacttgc ccaattaaat agtcagtcaa ttcatcaacc
    30421 atggacagat gccagcgcca gttccatttt tttaggaaac aaatatccca aacccatcat
    30481 taatcaccag gaagcgagaa cgaaggcatt gagttactat cagcaaataa aaaaaggaat
    30541 ggattgacaa gagactaact aaaatttggg gagatcctat tgaacaaaaa aagttggatg
    30601 gaactgattg cctggatcat tacctttgaa gcaatcggat tttttttagg aatgctgacg
    30661 caagcgaata tcccctcatc attgagccaa catcgtattg ccatttgaag aattaggctt
    30721 gagaacggtt ggaaacccaa tccatagtat tatcttccgc gacattgtaa atatctggat
    30781 tattaacaat aagggaatct tgtaatgcag aagtgagttg gcaatgtttt gcgccgtgct
    30841 tgactaaaaa taaaagaggg gacttctttt gctgatcttt ttttaagatt ttggctaggg
    30901 ctgtctcacc aaaatcatca acagcatcaa cagagccgac atgttgctgg caaaatttaa
    30961 ttagcaaatc actcatcaag ggatattcct cagatttgtt cgcaatggtc ttgattgcct
    31021 tgggaaagag gttcttttgc catggtgagt acaaggctga cattaatttt tgcgctacac
    31081 gatctgctcc tgaatccaat tcagcaatta gcatgctgat ttgctcatca taactaccag
    31141 tagcttggga taaaaaatct aaaaagagct gttcgtaatc tttttcggca taaaattccg
    31201 ctgtcatggg agtatctgct ttatcactcg ccagaaagcg cttaacaggt ttatctaggt
    31261 ttaagaaaat tcgatttttg ataattgctg catttttcat atcacaaatt actctacctg
    31321 gatatataat tttcattata atgaaattta agggatattt aaactatttc ccttttccct
    31381 ttacaacttt aaaaactggc tagtgttgtt acaatcttca ctcacctggt gttctctttt
    31441 tagaattcga tgcatattaa agcactctat tcgaaaatca tctgaaaaaa cttcagcctt
    31501 tgtactgatt attaatggag ggtccaattt cattttctca ttgatatgag atatgatttg
    31561 cgggttatct tttaaagcat caaggaaaaa acggatataa gaacgagttt caaatttatg
    31621 catcgctgtt ggccgagcaa tattttcccc tgaaaaatat ttttgcaata atcgcataat
    31681 accgctaatt gaagattgac tcgcgcaagc ctccttcaat tgattaagag catcgatgtc
    31741 tgcagtagtc gtacctaaaa acctgctgtc cagatgccta ttaagattat gaaaattaat
    31801 gcagtcctca attgatttaa gcacaatatc gcataatatt tttacttcag gagcgggggc
    31861 aggacaattt gcaagctgtt cagcctcgca gaaatctctc atacataagg gcatatagca
    31921 cgatttaaaa taatttagat agtacgccca tacttgatct cttaactgaa taaaatcaga
    31981 gtcaaattgt gcaatttgct taaagtattc atgataaaga ggaggatgag ttaaatattc
    32041 aagtccctcg tagcttggat aattaggcag aagattaatg cacttcacca ggggaataag
    32101 aatttcgttg acactgtgaa aaccggcacc gacgatatat gctataattc caaggctgta
    32161 ggcttgtatt aattcgatat tttgaggatt taaaagcagc agcatcatgt tcatatagat
    32221 agaagtcatg ccagaagggc cagtgacata ggggcaatca tttgtaagtg cagtcttgta
    32281 atatattgaa ttctgattgg cttcttgtgc ttgggcatcc gcacaccaag tttgttcagg
    32341 aataacggtt aaccccctac tatgcgctga atgatccagc ttcatcaatc ctaactggtt
    32401 ggtttttaag taggttttac gaggtccttg acgccctctg ttttctcctt gggtataaag
    32461 ctcatcctgg taaaataatt ttttagtgat gaaagttcta aattcttctg cttcattttt
    32521 agctgctata agctgatagg aaaagccttc ctcttcttct ttcaagtcgg ggtgatcctg
    32581 taaatattta tccagaactt gggcaaaaag gccagttggc ttaaatgatg gcaaatttcg
    32641 taatggaaat gggccgatta cactgcgagc aaacataaag gatatgaatt gaatttgaat
    32701 aaaattcttc ggattatttt cctttaaaat agcaataacc tcttccagag acggcctttt
    32761 tttaaaagcc ccaaactcta tatcagaacc ctctaactct tgcagaaaaa aattcaacgc
    32821 agctcgttcg gaagattttg agtctaattt actatactct tcgattaaat tgattatagc
    32881 ttgagatacc agatcaattg cttgcatcat gctttttgtt ggcgattcta tcaagcgaca
    32941 agccagctct gattcaaatc tccggggcag aatattgttt gcattagatt gactgtcttt
    33001 gataggcacc aagcataacg catttagctc atcggcatgc tcctgctcaa gcaagtgact
    33061 caagccatgt aacattttgt gaacgaccga agtatcttgc tcatgatttt cataagccat
    33121 ttttattgaa tcaatggctt tagctatcgc ttctcttttg tcgcccatgt aattggtctg
    33181 ccattgattt aataaaaaaa taaaataagt gtaagcctct ggttttgaat taaaacatcg
    33241 atagtcaccc gtgtttttta atgatgtcag atcagccatg gcagcaaggt tatcgctgcc
    33301 ttggcgtttt gtatttttta gagaagggtt aattttttct ttacttaaca tagaacgcct
    33361 tccttgaggt taatatggca aaatgatgac gttgcggttt tctttcctgg gttcatgatt
    33421 tgccaccatc tgctcttcat ttgctgcgat ggacgtcttc ttaatctcca ggaagagctc
    33481 ttttcgccta gagcttccta tttcgcactg atgcacacca atcgtatttt ctatccttaa
    33541 agtcaaattg aattgtgaaa gcagtgttgg gctttctttc aatacattaa agaaatgagc
    33601 aataaattta agttgcattt ctttatttat tactattccc tcttccgtct caaaagttcc
    33661 tttaaaatac gcctgaatct gtctaaaaat tgcggttatc cctacctgtt gttgacagac
    33721 atgacttaag acctcaagcg cctttgtttg cccacatggt gctgaaagga acaaacccat
    33781 atcgccagta cgcttaactt tatgctcttc taagcaattt ttcacagact tagacacaat
    33841 ttcaagcatt tcttttactt ctgagcttat atcctccatt gaggggattt gatgtggcag
    33901 acaatgatta cgcatacaaa caggcatata agtcttacgg aaatacgcaa ggtgctcctg
    33961 ccaaattttt tcatgaacct cagcaaactc tttatcaaac tcctctatcg ccttaaagta
    34021 atggttatat aaaggtgaag ctgtaagata ctctgttcct ttatgctgtg gataatcagg
    34081 cacaatacct acgcacttga ccatcggaat aagaatttct ttaatgctgt ggtagccagc
    34141 accaactaca taagtcatta ccccaaggct gtaagagaga atgacatctt ggtccttagg
    34201 gttgagcaga agaaacatca tattcataaa taatgaagtc atcccggaag gccctgtaat
    34261 ataagggcag tccgttttca gggctgagag ataatggatt gaatcgtagt ttggtaactg
    34321 ggtcttacaa tcagcatacc aggattgacc acttaacgtc agtagtcctt tggtatgaac
    34381 tgaatgttcg aacttcatta agccaacttg gttggtttgt agtctttggt taggtttgcc
    34441 ttcgcggcca cgattatccc cggtactaaa aatttcatct ccataaaaaa gctcatcaca
    34501 aatgaatgct ctaaagcggt atccctcccc tttttttact ttcgatgcgc tgtgatctct
    34561 aacagctgcc atgtgatgat agagctcaaa aagtgatgtc tcttcaggcg catattttgc
    34621 ttgatatttt tgcaatattt tcaaaaacgc gccttctggc tcatgttttg gcaggtcatg
    34681 caaggcaaga tcacgtaaag caacttgggc aaatttaaaa gaaatatgtt gaatttgtac
    34741 gaaatcctcc ggtctgtttt ccttgagtat ttttataata tcggaaatct caattttctc
    34801 tttgggggca ccaaaaagaa tgtccgattg aaccatttgt tcgagaaaat cttgtaaaac
    34861 ctcatgctca aaagattcgg taggggaatg ttcatattgc tccaggattt ttacgatagc
    34921 atctgaaact tgtttaatcg ctgccatcat tttagctgtt ggatttttta ttagctggct
    34981 cacaatgtcc gattcaaagc ggcgcggcaa gatgtcgtcg tgcccaagtt cttgttgctg
    35041 gacattagca agcgatttaa tcagtacaga gagcaaagat ttatgagaat taagcagtcc
    35101 gtccgcttta gataactgct caagcattga tgtaattttt aagacatcct gttcatggtt
    35161 ttcataagct acttttattt ctgcaatgat ttcatctaac tccttcttta attctggtat
    35221 ttcatagcag cttttccatt gagtgagcaa cataatcata taggtaaatg cttctggtct
    35281 cgatttgaaa ctctcaggga ctctttgtaa ttgcaggatg cgagaaaaat aaagtgttcg
    35341 tgctgatgtt tcatttgaca tgagcccagt aatacacgcc tcgatttcag catgcaaaag
    35401 cacaagatgc ttatttttaa ttcctatgtc cactttcgca aacttaacct gcctgaatgg
    35461 attaaaaagt tcatccagag aataggccaa agcaaaaaga tcgctagcct gagagaaagc
    35521 aataaaatcc tcttgtgcat taagttcagg tgctaaatag cttgcggttc cccatgatac
    35581 cggtttaggt tctttatccg aagaactgct gccgaaatca actaaatgac actgcatatc
    35641 cgcatccagc ataatatttc caggtttaag atcgcggtga attaagccta gctcatggaa
    35701 ttgtttcacc tgatggcata cagaaatcat aatgccgaga acttcccaaa aatgttttag
    35761 attaacacta agaggaagtg cgtttttata ccgggataaa tcaatccctt ttataaaagt
    35821 ggtgagaata taatgcacct gagtttgttc attaaaaaaa gttcctacca aacgacccaa
    35881 tttgcttaag gtatctttct cattggtaaa ctccgtcacg ctttcagcat cgatcttaac
    35941 ggattttagg aaataagttt ttccatcgtc caaagcataa actttaaatt gctcacaata
    36001 agatttacca cctcctgaaa tcttgttttc ggtgggaacg atgattggct taagagatgc
    36061 cccggtggta ataaacagaa tttgacaatc tattccaaac tcatgaatca ttatttcgcg
    36121 tgagataggc ttgccgtcgg cagattttat tagttttcta atgcctttgt tgcctaaaac
    36181 ctttatcaac aattctaatg ctttctcgtc catgcttggc aatgatttta attcatgaaa
    36241 ccgaagcaat ttcataagga acctcacata tttacaaatt tgaataaaaa tgacttgata
    36301 ttataaaaaa tcatcattaa gagaaaatta agtaaatatt gagagcaacg cacaaagcga
    36361 aataatagcc cccaaacatg ttctcgcatg ccaaatggct gctcccaaga tggagctaat
    36421 taagagaatg attacacaaa tatcaaacac cgtgttcagg tatcgtaatt accgataccc
    36481 ttttatagta agaagcattt atctcgatta gccataaaac taactttttg ttaaatgata
    36541 ggttggggaa atgaagctct attacctgaa tccgattgaa tgtaaaatac aaaaccagaa
    36601 ggataaaaat catcattaag ggaataaaat gaatggatgg acgcttaagg tttttgcgat
    36661 tgcctttttt atctttattg tgacagatat gatatggctt gggttcatcg ccagaaatct
    36721 ttattttgac caataccaac agtggctgcg tctttcagag gggcaattaa agccaatatg
    36781 gtggtctgct ttattagttt atctgctttt tgccttgagt attgttgtct ttgttatacc
    36841 gctggctaaa acatcgctac cttatgctgc attatatggt gcgcttcttg gtgctattat
    36901 ttatggaatt tatgatttta cctgcctcgc tatttttaag gattttccag tcggtatggc
    36961 attccttgat tggctatggg gtatagtact gtgctcctgg agttcgatag cgactatcta
    37021 tgtggcaggt tatatcaaat aagacatcag agattggcag gatccaagtc tgccagtatt
    37081 cgttatgctt tatgggtaat ctccttttgt ttattaaggc ccatttttgc caattgatat
    37141 caacgtagtg aagataagga attctatgca atttttattg attctgttga ataaaactcc
    37201 attagagagc gttgctgagt ggcttaatat aaaagccatt gtctcaattt attcagctgg
    37261 ccaagattgt agtcccttgc tagtcatata agcaatcgtg atgaacatta atccattaaa
    37321 ccttgttcac tatgtggcgt actatctatg ctggatattt tgtgtctatc tggcctctta
    37381 tggttacccg ttattgggat taatcattac tctattaatc atggctgtac agattattgt
    37441 acagatactc aatcagctgt cctggcgtga tgcactctgt ttttccattg gattagccat
    37501 attaggtgca atcactgatg ccatctggct ttatagcggt atgatctatt ttaaagccaa
    37561 tttctttggc tcccattttc ctccgctctg gatgttcgcc ctctggttaa gtttcgggtt
    37621 taatctcgtt cttttgtata gttcctggtt aactcactat tttctttggg ggttagtcat
    37681 gtttccatcc attatctttg cgtactggct aggggtacaa tttggagcgg caattcttat
    37741 cgggaatgaa attgtatttt accttactct agggttaacc tggtctctat ttttgccttt
    37801 aagtttgtac ctatttaatt accagtatag gaaatgaata agatgataat cttactcgtg
    37861 atcgtcatca tctttttaca tatgtgccta atttggctat ggtaccgtta caccaataat
    37921 ccttcggtgg ttgatgtggg atgggcttct ggtttaacac tggccggtct tgtttattta
    37981 aataatcaag agccttcatt gcgctttttg ctgcttagcc tggcattact tatatgggga
    38041 gtcaggcttg gagggtattt atggtggaca cgaatacgcc ttaaaaaaat agacaagcgc
    38101 tatcttgctt taagcaacga ttggaagctc gccaaacctt tgggattttt tttaaatttt
    38161 cagttgcaag gcgcattgat ttgccttgtt tccattccct ggtatttttc ttcctcagga
    38221 actcaaacaa cgctcagtct attggatatt ctcgcattga ttatatttct aatggcctta
    38281 attttggaaa cagttgccga taaccaattg caagatttta agaaaaatta tcctggaaaa
    38341 gtctgcaatc aaaaactttg gcgttattgc aggcatccaa attattttta tgaatggctg
    38401 gtatggtgct cctttacctt atttgccctt tccgctcctt atggatggat agccattatt
    38461 tctccattga ccctttattt aatcatgaca aaaatcacag cccccatgac tgaacaaggt
    38521 tcgattgaat ccagaggcaa tctttacatc gaataccaaa aagtgactcc catgtttttc
    38581 cctaaatggc taaaaataat tcgctggtaa gaaaggagct aataatactg atgatttgcc
    38641 tcaaaaaagt acccggcata aggggtatag tgacaagtat gggatagtaa taataaatat
    38701 taattcccat tacataatgg cctcctctat ttgtaaagga aatcacacaa caagtcccat
    38761 aaactaaagg ataaattttt tagcgattga aagtgagaat agcgtgactc ccggttcttt
    38821 aagtaatgtc agcagcattt acttcattat tgactttatc gcatttgaaa taaacgctat
    38881 tcaacttagg caaaagctgt tctgagaaaa atggttcttc tgtattccaa aataaaatga
    38941 aagctcctaa atactctttt gaaaaagaat agaagtcgtc gaaacgatag aaagcgcctg
    39001 tcttaggatt ggtataagta taatctggtt cttgaatagc catggctgtt tctatctttc
    39061 ctttgaattt gtgaaaaaaa ggataactgt ttttcatttg tgattcttta taaggcacca
    39121 catctggtcc gcctaaaccg atgtggtgtt ttctggcaaa agcaaataat ctgctcatat
    39181 agtgatggtc attattccac tcatctggaa aaaaattaac atattgaatc acccaggttt
    39241 tttgaaagat cttttttaaa taaccaatat tatcaagaac tgaatagaaa tattcgtctg
    39301 agctaaaatc tttggggaga ttatcagga
//
LOCUS       DATNSN010000013        35239 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.013, whole genome shotgun sequence.
ACCESSION   DATNSN010000013 DATNSN010000000
VERSION     DATNSN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35239
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.013"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(74..1204)
                     /locus_tag="VAH76_000539"
     CDS             complement(74..1204)
                     /locus_tag="VAH76_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEO1385189.1"
                     /translation="MRGFLLALLLSINTAVWADELVGFAAFENGDYTTAYPHLMQAAK
                     EGNEEAMYLLGRMYQYGYGVTTNYEEARNWYQKAADKNNALAQLSLGFMYDTGKGVSQ
                     DFAEAFKWYMKAAEQGNPIAQRNIGLMYATGDGVAASDDKAFTWFKKAAEQGYSKAQV
                     NLGYQYMMGKGTPKDVKKAFEWYQKAAEQGDEKGEYSLGLLYTGQEGGISADDKAAFY
                     WFSQAANHGHVNAQTYLAYYYLKGYGVDADPVKAAYWYQSAAEKGQPEAQAQLGQLLL
                     TGTGVDKDYQQAAYWFGKSAHQGNPVGQAKLGYMYLAGLGVNKSLVKAYAWLKIAAEN
                     KNEEAAKQLKSLEAKLTEPEKLEAEKMIKDLGPLESKENYED"
     gene            complement(1562..2746)
                     /locus_tag="VAH76_000540"
     CDS             complement(1562..2746)
                     /locus_tag="VAH76_000540"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEO1385190.1"
                     /translation="MTDVYVVDVLRTPVGKAPRGVFKNTLPDDLLAHTIKCLIHRHQN
                     IDWQEIADVVIGCAMPEAEQGMNVARIASLLADLPQSVPAMTINRFCSSGIQSIVTAA
                     DTIRNQEAHLALGGGVESMSMVPMGGNKYTANPSIFNDEHVAIAYGMGITAENVAKRW
                     EITREQQDEFAIESHKKAIAAQERLDFNAEISPIEISIRYPDLKTSKVEIKKKLINKD
                     EGPRADTSYEAISKLKPVFALNGTVTAGNSSQTSDGAGVALLASEKALKQYDLKPLGR
                     LMSYAVAGVPPEIMGIGPVKAIPMALKRAGITLDQLDWIELNEAFAAQALAVIKDLDL
                     PREKVNPLGGAIALGHPLGATGTIRTATLLHGLRRTKGRYGMVTMCIGTGMGAAAIFE
                     SL"
     gene            complement(2762..5131)
                     /locus_tag="VAH76_000541"
     CDS             complement(2762..5131)
                     /locus_tag="VAH76_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEO1385191.1"
                     /translation="MQEPFFIKKVAVLGAGVMGAQIAAHCVNAGIETLLFDLAGKEGP
                     KNSLVDKAIANLGKLKPTPLATPQTNELLQAKNYAENLEELSSCDLVIEAIAERIDWK
                     EDLYKRISPYLSDKTILVSNTSGLSINKLCEVLPAQHRSNFCGVHFFNPPRYMHLAEL
                     IPANSTSSNLLDYLETWLTEYLGKGVVRAKDTPNFIANRIGVFSLLTTLHHTLAMNMG
                     IDEVDALTGPLLGRPKSATFRTMDVVGLDTMQHVINTMQSQLTADPWHKMFILPDWLN
                     GLIKEGHLGQKSGQGIYRKAGKVIEVYDVQTGQYRPSRAEVSDELKAIMDIKDPEARM
                     KNLMTSNNKQAQFLAACFRDLFHYCAYHLEEIADNARDVDLAIRWGFGWSQGPFETWQ
                     LSDLHSVTRQIDESIQDNTALSSAKLPEWLYEIKEFYTREGAYSPGKNNYHARSQLPV
                     YHKQFFHDRVLKESHHPVHVIYENEGVCLWYLKDDVAVISFKSKANTIGQSVLDGIET
                     ALDIAERDYQGLIIYQQDASNFSSGADLRGVSKLLTDGTIQALEHMVEQFQRVAMRLK
                     YSSIPTVAALRGRALGGGCELMMHCDSIVAAFESYPGLVEAGVGVIPAGGGCKELAMR
                     SAHHAQQADLLTFILPVFQQIATAKVAASAVEARNMGYLRAADSILMHADEVLYASLE
                     KIKAMRAANYLPPMKKQRFKVAGIEGHARLQSGLVNWLEGGFISQHDYFLANELAKVL
                     CGGDVNHGTLVDEQWMLKLEREAFIKLANTSLTQARISHLLETGKPLRN"
     gene            complement(5251..6771)
                     /locus_tag="VAH76_000542"
     CDS             complement(5251..6771)
                     /locus_tag="VAH76_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEO1385192.1"
                     /translation="MDLLQRLNIKSVNPGAFSGHGWHSDNHAHTLESFNPSTGNKLAE
                     IATCTMDDYEQVMQRAEQAAQAWRKVPAPKRGEIIRQIGQALRENKDSLGSLVSLEMG
                     KSKQEGDGEVQEMIDIADFAVGQSRMLYGNSMHSERPNHRMYEQWHPYGIVGVISAFN
                     FPVAVWSWNAFLSAICGNVTIWKPSAKTPLCAVAVQHICNQVLKENNCPEIFGLIIPN
                     SHDVVEAMVDDKRIQLISFTGSTAVGKQVAAKVAARLGKSILELGGNNGIILDESADL
                     NLAIPAIVFGAVGTAGQRCTTTRRLFVHESKYQDVIKRLRHAYEQITIGDPLDTRNLM
                     GPLIDQQAVEQFKKAINRIKAAGGQIVYGGEILKQAGSFVQPTLVCDVKNDWDIVQEE
                     TFAPILYVMSYRTLDEAIALHNGVPQGLSSALFTQNLKNAELFLSACGSDCGIANINI
                     GTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINWGDELPLAQGIRFNLS"
     gene            complement(6781..7869)
                     /locus_tag="VAH76_000543"
     CDS             complement(6781..7869)
                     /locus_tag="VAH76_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEO1385193.1"
                     /translation="MYNVMITGAGKIGSLIACLLADSGSYQVHLADLEFNGSDVTRLL
                     TALPEIKTVALDVKDEQSTQAYLQKHNIIAVISSLPYFLNTHVAKAAKAAKAHYFDLT
                     EDTSVTEAVKAIAQNAETAFVPQCGLAPGFISIAANSLMQEFEKCHHARLRVGALPQR
                     ANNALHYSLTWSTDGVINEYGNPCYGIEGGKDVVMAPLEGLESIQIDGCEYEAFNTSG
                     GLGSLAELYAGKIQSMNYKTMRYPGHCKKMRFLMNDLRLNEDRGTLKRILENAIPKTY
                     QDIVIVYVTVEGIKQGELTEKSYVKKIYPEVIRGLEWSAIQVSTASGVCAVVDLVLGQ
                     GNEYKGLILQEKFKLSHVLENRFGKYYA"
     gene            8023..9558
                     /gene="lnt"
                     /locus_tag="VAH76_000544"
     CDS             8023..9558
                     /gene="lnt"
                     /locus_tag="VAH76_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEO1385194.1"
                     /translation="MKQAALIHSTIINTSTLNQYSKFIVVFLAGLLAPLGFAPFHLPG
                     LTILSLAILFSKFLQCTVKQGFTLGFLYGLGYFGFGVSWVIISIHDYGQLDYFLAGVI
                     TLLFIMYLSLFPGIVGYSFKLLSAKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLT
                     GITQIDTPLRYMAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPS
                     LGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWDLLKYYENKTDKLLGKNLIVLP
                     ESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNSIISLGEATGAY
                     VKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYP
                     NILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVI
                     NHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPAFIFCFIFVLFVLFFNIKRF
                     TEK"
     gene            9624..12095
                     /locus_tag="VAH76_000545"
     CDS             9624..12095
                     /locus_tag="VAH76_000545"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO1385195.1"
                     /translation="MDNTYNPQEVEEQAQQYWHKKQSFNVTEDLNKEKFYCLSMFPYP
                     SGTLHMGHVRNYTLGDVIARYQRALGKNVLQPIGWDAFGLPAENAAIKNKIPPAEWTR
                     KNIAAMKEQFLRLGNAYDWKRELTTCDPEYYRWEQWFFIRLFEKGLVYKKNAVVNWDP
                     VDQTVLANEQVVDGRGWRSGALVERKEISQWFIKITSYADELLSSLDSLDEWPAQVKQ
                     MQRNWIGKSIGTEIYFNVNNYPKRLKIYTTRPDTLMGATYLAVATDHPLAKEAASNNK
                     KVQEFLDSCQGIKIAEAELATMEKRGIDTGMTAIHPITGKELPIWVANFVLMQYGSGA
                     VMAVPAHDQRDWEFAQKYQLPVKQVIKPIDIEHDFNQSAYTEEGILINSNQFDNLLSS
                     KAIQVITNFLEENDAGKATINYRLRDWGVSRQRYWGTPIPMIICEQCGIVPVPDEELP
                     VVLPENVDFTGTGSPLTQCKEFVNVTCPKCGQDATRETDTFDTFVESSWYYARFACKG
                     QENAMLDDRAKYWTPVDQYIGGIEHAVMHLLYARFFHKLMRDEGLVNSDEPFKALLTQ
                     GMVLKDGHKMSKSLGNVVDPNHLINTYGADTARLFVMFASPPEQSLEWSDSGVEGAHR
                     FLKRVWAFSHQHRDMLIDINDSILSGNGHVDWKEAESRLKKSRHIVHQILAQATHDYD
                     RNQFNTVVSGCMKLFNEISDYSIETENDKFFIHSSISILLRLLAPITPHICHCLWQQL
                     GFDKAIIDAPWPKVDKSALKTDEVDYVVQVNGKLRAQFTASTDATEEELIAAAKEHAH
                     NFVVNHTIKKAIIVPHRQLINLVIG"
     gene            12196..12687
                     /locus_tag="VAH76_000546"
     CDS             12196..12687
                     /locus_tag="VAH76_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385196.1"
                     /translation="MTQIHKYLASLILIWLLTGCGFHLRGAIDIPEWLDNVSIISRDG
                     NKELVSILKSQLEGYKIDVNSDPSLAKYWLIINSSSLQQQIVSIGASTNPRQYQLILT
                     IEFMLQTPKGQIIKTPRKVNVSRQLTVNNDRILGSNEEQALLVSEMRRDAAIQIINRL
                     NRP"
     gene            12689..13714
                     /gene="holA"
                     /locus_tag="VAH76_000547"
     CDS             12689..13714
                     /gene="holA"
                     /locus_tag="VAH76_000547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO1385197.1"
                     /translation="MQINQQALTQLLQKKIHHLNILIGQDHYLIDEALNNIKSMLKKS
                     SECDEKILSIQSPEEWHVVLEETNNYSLFYQTVILTIFFDKKTIDTTGKKILSEYLKS
                     ANSHSYIIIRAPNLTAKQLQWLTHDEQALIIIAQPLSSEAMKRWINEQLKLNTISFDP
                     YVPELIYQYTKGNMLATAQVIEKITLSNTPRTLITPYLALEHLSDQCDFSLFELIDSC
                     LLGHSDRAIHILRQAANNKTEVTLVLWILTQEIRTLLQLLFLMQQKMDIKMASNQLKI
                     WTQRVNLYQSAVKRLNTLLLQQLHHYCQSIDDKIKSNSNNQAWNALENISLSLCLGQL
                     LGDACIA"
     gene            13702..14337
                     /gene="nadD"
                     /locus_tag="VAH76_000548"
     CDS             13702..14337
                     /gene="nadD"
                     /locus_tag="VAH76_000548"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO1385198.1"
                     /translation="MHSIAIFGGTFDPVHNGHIKTSLAIQANFGFDSYYFLPCKSPAI
                     KPPSFASSEQRVEMLKLALKPYPDFKIDTRELDRDTPSYMVYTLQSFRQEYTDSSLTL
                     IIGYDGLLNLPQWYQWEKIISLANLLVINREEFFQQPVPKSVQTLLNQYRNDDKNILL
                     NHHAGSICLYNAGHYDISSTKIREQLKQHKDVKNNLPDLVYNYIKKQGLYQ"
     gene            complement(14312..14845)
                     /locus_tag="VAH76_000549"
     CDS             complement(14312..14845)
                     /locus_tag="VAH76_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO1385199.1"
                     /translation="MQLQWVDIAIVAIIALSVLTGLFRGFVKELVALCVWILAIWLGF
                     NYSQSLDPWLSSYIEEQSVRTAIGFIIILFATLFVGGVVNAILSFILKRAGLSGTDRT
                     LGMGFGFLRGVFIVALIMVAVKMTSLPYQEYSQKSKLYARFDPVVALLYSHLPEFIKQ
                     VKAVDKTENIIDTVPAS"
     gene            complement(14848..15618)
                     /locus_tag="VAH76_000550"
     CDS             complement(14848..15618)
                     /locus_tag="VAH76_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEO1385200.1"
                     /translation="MKLIMDEKVKHRLIGLAVIISLGAIFAPALIKKSSQSPESNFSV
                     NVKLPPKPLPPDVVITDEKEVFKTIKVAKTEIPPIPVEKKSINLAKAEPISTEEDNLI
                     RTSKVATLEAERENSIKSEPIKLAVNKAANATAKKVMTLAAAKPPVKTSPVVTAKVNT
                     NKKVSQIVAARKPAVKQDIYAVQLASFTQIANAQALVNRLRSKGYKANYLKSNSRNGI
                     VYKVVVGHSPVKKDVIKLKTQLASAMQLNGFVVNTGVS"
     gene            complement(15615..16901)
                     /gene="folC"
                     /locus_tag="VAH76_000551"
     CDS             complement(15615..16901)
                     /gene="folC"
                     /locus_tag="VAH76_000551"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO1385201.1"
                     /translation="MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRP
                     NCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTSPHLLVFNERIRINLTPIQDKDL
                     CKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
                     AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSY
                     LYGKDYFYQEEADHWGVTCQGKPFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNL
                     KKAMELIYIPGRLQLQKGKTDILYDVSHNPQSVRLLADTIKKINVKGQIHAVFSALKD
                     KDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMA
                     LKQASDDDLIIVYGSFFTVSHVMAAQHNLLEQKENQ"
     gene            complement(16882..17766)
                     /locus_tag="VAH76_000552"
     CDS             complement(16882..17766)
                     /locus_tag="VAH76_000552"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO1385202.1"
                     /translation="MSWLKKLLPSRIRTETSQKKGVPEGLWVKCSGCNEVLYSTELAK
                     NFMVCPSCNFHHRISARLRIAQFLDESGQEEVAAGLEPQDRLKFRDSKKYKDRISQAQ
                     KATGEKEALIVVKGFLMQQPVVVSAFEFNFMGGSMGATVGEKFVRAVNVAATEKRPYI
                     CFTASGGARMQEGLFSLMQMAKTSAALAKFAETGLPFIVVLTDPTMGGVSASFGSLGD
                     IIIAEPNALIGFAGPRVIEQTVRQTLPEGFQRSEFLLEHGHIDMIVERKHLRATISEL
                     VSKLTHKGAESFFAHAME"
     gene            18178..19605
                     /locus_tag="VAH76_000553"
     CDS             18178..19605
                     /locus_tag="VAH76_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947070.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEO1385203.1"
                     /translation="MAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDD
                     AAGLAISQRMTAQIRGMNQAVRNANDGISLSQVAEGAMQETTNILQRMRELSVQAANS
                     TNNSSDRASIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTINFS
                     IGSIKASSIGGIATATGTEVAGAAATDITIAIGGGAATSINSSANFTGALNGQDATSA
                     YAKAAAINDAGIGGLSVTASTSGTQAVGAIGGTAGDTYNLTINGVAIYTNLDVATALT
                     NSDLRDAINGVSNQTGVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGA
                     FDGALRGTLSISAVDTIAIGGTVANIGLSANISKDTVGIDSLDVSTASGAQTAIKRID
                     AALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQI
                     LQQAGTAMLAQANSLPQSVLSLLGR"
     gene            19688..19969
                     /locus_tag="VAH76_000554"
     CDS             19688..19969
                     /locus_tag="VAH76_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385204.1"
                     /translation="MSIEPVPSVNRAAQTDQIKIVEEASKPEIKNMVYDSALDAETKQ
                     ALDKATGLLQTIITEKISDKVLRKMPSDEYLQLLSLLDDIISGSIDKHV"
     gene            20032..21657
                     /gene="fliD"
                     /locus_tag="VAH76_000555"
     CDS             20032..21657
                     /gene="fliD"
                     /locus_tag="VAH76_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEO1385205.1"
                     /translation="MELSSPGVGSGLDVKNLVDAIVTAEITPSQVRHDKQLNSVNTEL
                     SALGQLKSYLTNLQTSLAKLSSLSQFYTMKSTISDPNYFSATLGTGATKNTYQIEIQK
                     LAQQHSLASSYMTNTGSGTITINFGTYNSDKTTFTPNPSATSVNITIAPGNDSLVAVR
                     DAINNTNSGVTASIVQDSQGSRLTITSSKTGENYAMKISGGVASLNYDPTMGVNSLTE
                     TVAAQNSTVKINGLTLVQSSNQLNEAITGVTIDLKKAEVGKVVTLTVDDNKDQLTGLI
                     NEFVKQYNDTMTFLTNLTGYNSETRQGGVFQGDPQFRNLKLNLNKWATTPLANQEGPI
                     RSLADLGIITNKQGLLEIKQDKYKAALDNHYKEIGALFAKTAKANDTGVNIKAINSTV
                     KSGTYDVVLTEFTPGVSMSGTIGGLSATSSDGITLTGSGDLSGLSINVFSGSAGARGQ
                     ITVTDGIAVQLSSLLDTYIEIKGDLDQRKDQLNNQVKQLNKIQNEIDIRRKSIETRYL
                     KQFTALDTLLSQLQSTSVFLSQQLANLPQLKLK"
     gene            21667..22077
                     /gene="fliS"
                     /locus_tag="VAH76_000556"
     CDS             21667..22077
                     /gene="fliS"
                     /locus_tag="VAH76_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEO1385206.1"
                     /translation="MRMKNLYSQISSQYKAIELQTRIETASPHELIDLLLQGARSHIA
                     TAQGNIQRNQIKEKGEHIGKAISIIEGLKMSLNHDKGGEIAENLLQLYDYVQQILLKA
                     NLKNDEDLLAQSNMLLAEVHQAWQAINSNAGNEL"
     gene            22400..23146
                     /locus_tag="VAH76_000557"
     CDS             22400..23146
                     /locus_tag="VAH76_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1385207.1"
                     /translation="MNTFKSSLAFVICLLLGMQSAQAQMRYGETLCNLPEYFCMKIKS
                     GDTWDRLFPNIEEQDIVRRINRMNIRLKPGMIIAVPKNIERLTIYDVSPFPRYIEPSG
                     EKTIYVSQKKLAWGAYDEDGELLWWGPISSGVGKCKVIGGCSTPTGSFRIIRKQDIDC
                     VSTVFPRRANGEHGGALMPYCMHFFRGYALHGSYEVPGYRASHGCVRMFIEDARWLNE
                     EFIDLPGGGMRGTRVILDAPDAQENPDNLQ"
     gene            23387..24685
                     /locus_tag="VAH76_000558"
     CDS             23387..24685
                     /locus_tag="VAH76_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385208.1"
                     /translation="MAHYNVQSESLNKASVSLIMPWMVWGLGCLFYFYECLLQVSPSV
                     MSNELMRDFSVTSHTLGILSGIYFYSYAAMQLPGGVLMDYFGPQRLLTLATVVCAVST
                     IAFGMTDNFFMACVARLMIGFGSAFAAVGTMKLAANWFPAQKFALLTGLMVTIGMMGA
                     IGGETPLALLIDNFGWRESMIIMGSVGLLLATLLILIARDTPKNFSPAIHQHPVEEER
                     LIPSLFTLIKNKQLWLVATYGGLMYMATPVFCGLWGVPFLMNKMLIAKSTAANFISLV
                     FVGWAIASPLWGLFSNRIGLRKPPMYIGCVGAMICSLLFIFAPITSSITMEILLFAFG
                     IFSAGFLPAFSVAKELCNKKYVATGLSFMNMMNMVGIALAQPLIGYILDKMWQGQMMG
                     TVRVYPLEAYYTALSILPIGMLVALLILPKIKETYCQSVN"
     gene            24722..26065
                     /gene="miaB"
                     /locus_tag="VAH76_000559"
     CDS             24722..26065
                     /gene="miaB"
                     /locus_tag="VAH76_000559"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEO1385209.1"
                     /translation="MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVI
                     LLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIV
                     FGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADL
                     ALLIHYIAAIDGIGRIRFTTSHPLAFSENLINAYAEVPELANHLHLPVQSGSDRILSL
                     MKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSF
                     SFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGF
                     SKKSSQQLSGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA"
     gene            26065..27030
                     /gene="rluD"
                     /locus_tag="VAH76_000560"
     CDS             26065..27030
                     /gene="rluD"
                     /locus_tag="VAH76_000560"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEO1385210.1"
                     /translation="MIEHIQQQLSVPRELHNQRVDSVLARLLPEYSRSLICNWIKTGA
                     VTLNNLPCKPKDKALGGDLITINVEHTIVNTDFNLCKPENIPIQIIYEDSEVIVVNKP
                     AGLVVHPGAGNKEHTLVNALLHHCPELQLLPRAGIIHRLDKDTTGLLIVAKNLISHTS
                     LIRQMQNREIKRHYLTLVHGYVISGGTIDTGFGRHPKNRLKMAVTDFGRQAVTHYNIN
                     KHYKDFTLLNVSLMTGRTHQIRVHMNYINHPVVGDQLYGGRMKFPPNADEAILSVLKQ
                     FKRQALHACSISFCHPVKNTEITLNALLPDDFELLLNTLDQHDEK"
     gene            27020..27745
                     /gene="pgeF"
                     /locus_tag="VAH76_000561"
     CDS             27020..27745
                     /gene="pgeF"
                     /locus_tag="VAH76_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEO1385211.1"
                     /translation="MKNRFANWPAPENITALSTTRLYGFSDGPYASNNLGLHVGDNQQ
                     HVLKNRQQLIELIDLPAEPIWLEQTHSTKCITVETEKTRDADAAITRLPKLPLVIMTA
                     DCLPILLCNRQGTEIAAIHAGWKGMYNGIIENTLKKLNSNRTDIIAWIGPAICKKCYE
                     VGEEVYLSFTNKYPLTRNAFEPVHSKWLADLPQIAEIVLNLEGVKSVFQSNLCTFELK
                     NEFYSYRRESQTGRIGTFIWINE"
     gene            27762..29138
                     /locus_tag="VAH76_000562"
     CDS             27762..29138
                     /locus_tag="VAH76_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEO1385212.1"
                     /translation="MITRIKLFIASLLVFFIPVADAAQDLTNMPSLAPVLKNAMPAIV
                     NVAVQGYLPNNMASGNADDDDGENSKQPSRIPEKGRKFESIGSGVIIDPKNGIIITND
                     HVIRNANLITITLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLVIGDSDKLEVGDY
                     VVAIGNPFGLNSFGNSQSATFGIVSALKRSDLNIEGVENFIQTDAAINPGNSGGALVN
                     AKGELIGINTAIISPYGGNVGIGFAIPINMVKDVAQQIIKFGSIHRGLMGIFVQHLTP
                     ELAQSMGYAEDFQGALVSQVNENSPAQLAGLKSGDVIVQINDTKITQATQVKTTISLL
                     RAGSTAKIKILRDNKPLTLDVEVTDIKKHEQKLQSNNPFLYGLALRNFEQESPPHGNV
                     VGVQVVGASETSAGWRAGLRPGDIIISANKTPVKDIKSLQAVAHEKKKQLLVQVLRGA
                     GALYLLII"
     gene            29448..29524
                     /locus_tag="VAH76_000563"
     tRNA            29448..29524
                     /locus_tag="VAH76_000563"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29482..29484,aa:Met,seq:cat)
     gene            29737..30873
                     /locus_tag="VAH76_000564"
     CDS             29737..30873
                     /locus_tag="VAH76_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1385213.1"
                     /translation="MFRINLIRYVSLLFIITLNLDCISVAFSSQIIYGTQNAARVTGK
                     NLKRIYFLQLGAFTQKNNAQQLARKIQARTQYPVIISHSGNAFSVRVGPVNTQELHQL
                     SIKLSQNPNITPIKHNSSPKQIPTIQTKSQILVKETSRPLPTHSVAKDTIDGKGSWYV
                     TAKIGAQETTPDDQFTVNNDSGFPSPFDKDIYTNSSDSSLLLGIGGGYRFTNIILPAV
                     SVGIYYSHFFNMDINGQVIQYSLPEFTNYNYQWKIKSDLVLASVKANVFRWKNLMPFV
                     QGGVGASSNRASQYYENALPDVTPRISPNFANHTATVFAYQLGAGLDWEIRPQWLLSL
                     GYEYSDLGKVRSGYGTDSWNGETLSSKNLTSNAGVLGFTYLFHG"
     gene            30926..33406
                     /locus_tag="VAH76_000565"
     CDS             30926..33406
                     /locus_tag="VAH76_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin domain-containing protein"
                     /protein_id="HEO1385214.1"
                     /translation="MWFPNNNLSNRTDLIFIKIMGERTNMKYSTRIIITTICMLFALL
                     GYAGKDPISWRVSGVFPAQSSVGQTYTVTYTFTNQLPFRLAKALIIERSASPNTEFNF
                     NDQCSGRLLNSGESCTVVVALNPLVIGLKTLQLTIAGYSKDRVPLPQTSTLSTGQSLS
                     PIVSFVMDGFPSQLTTGSVSSYKFTFTNNGAISATNLSVNVSQSLGAPNFTTTCNNTL
                     TPNGGVCEVDGTYTVTSGSPSIQQVIASLSYTGPSGSPVTAQTFTQVVNPAAPLVGTL
                     VPPHYLPPLMVENTIYTVQFLFTASGTVTIDNPGGSIACSQGVNPCNVGVMNITSSSC
                     TAPQTLTNAACQMTATFTAPPATIPPAAPLTYILTATVPYSVGGNPQPDATAITSGTV
                     LTALPTERTVTLINECDFDVWFSLNGSQLGNSPNCPTTPCPNGTSCNTSTNKCFWNNP
                     APNNGVYLLQALPGTNTNSVAIPVTNADPNIQWSGNISASTLCNGTTCQQAACDNNGG
                     TTSCAPGIGFKQPATQAEITMNLTTSDSYDVEVINGFHIPISMQPIYYQGVTTIPAIP
                     DNYNCGEPGKDTAANGFGACDWSTATVPVIDQVPGNGFYWVTGGGQGCSITSANPGCP
                     AMTLCGLDSNFNQVCGNFLGYWSADQVCGSSNVPAAVQSYFKCNQPLPTSTTPFYPSG
                     AVLSNLMLCSVSTGFTGPRYNTCYNAYPSSSPTDIAQCCGCVDWWNTAQTNNVAIGAN
                     PNTESCTQPGASQPQTNAQWNSFVQPMIQWMKRACPSAYIYPFDDKTSGFTCTNNLSG
                     QPNSTSYIIRFCPGSITGLPAGVNEGRG"
     gene            complement(33486..33794)
                     /locus_tag="VAH76_000566"
     CDS             complement(33486..33794)
                     /locus_tag="VAH76_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEO1385215.1"
                     /translation="MFSNDITIDPEIFVQFQAWLKKHVAEIIQFPGFMQACILKQEKE
                     TKSGKEKLTVQYQLDNRDNLSRYLSELAPKMREEGIHLFQAKFFADRRIFEVQEIISK
                     "
     gene            33897..34919
                     /gene="istA"
                     /locus_tag="VAH76_000567"
     CDS             33897..34919
                     /gene="istA"
                     /locus_tag="VAH76_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family transposase"
                     /protein_id="HEO1385216.1"
                     /translation="MIHILYKQGYSKKAIARKLGMSINTVRKYLKSGSEPYYKRRDKK
                     PLKLDPYKAYIQKRLKDATPHWVPATVIYREIKLFGYDGCSSQLRAYMHTLRSKKEEV
                     IVRFETEPGQQMQVDWAQFRRGKDRLSAFIATMGYSRASYVEFVSDETLETLIACHKN
                     AFEYFGGVPYTILYDNMKTVIIERNGYGPSQHRFQAGFWDFAKHTGFRPKVCQPYRAQ
                     TKGKVERFIHYLKYSFYFPLVGQLKALGLSLDKETANMHVLKWLNEIANQRVHATTGA
                     IPFERLLDEQAKLQPLSSTYSGKLFSHLENKEVHYFAFQLLDNTAMQHELSIYQKLLE
                     RTEEAA"
     gene            34916..>35239
                     /gene="istB"
                     /locus_tag="VAH76_000568"
     CDS             34916..>35239
                     /gene="istB"
                     /locus_tag="VAH76_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family helper ATPase
                     IstB"
                     /protein_id="HEO1385217.1"
                     /translation="MNIQHERILSLCERLNLSAIATNYSYLAQEAAIHNQGYSDFLES
                     VLTVELQEKQYRSRTLLTKMAGFPMIKTIDDFDFKFASGVPKNKIRELTSLAFIERQE
                     NILLLG"
BASE COUNT        10942 a         7244 c         6496 g        10557 t
ORIGIN      
        1 tttaaaccag tattttaaac acacaacaaa ttattaagtt acaactagta tcttctatat
       61 ttagttttta actttagtcc tcataattct ctttgctttc taatgggccc aaatccttga
      121 tcattttctc tgcttccaat ttttcaggct cagtcaactt ggcctccaaa gatttaagtt
      181 gttttgctgc ttcctcattt ttattttcag cagcaatttt taaccaggcg taagctttaa
      241 ccaagctctt gttgactcct aatcctgcta aatacatgta ccccaatttg gcttgtccaa
      301 caggatttcc ctgatgtgct gactttccaa accagtaagc tgcctgttgg taatctttat
      361 caacacctgt accagtcaat aacaattgtc ctaattgtgc ctgtgcttca ggttgcccct
      421 tttctgctgc agattgatac caataagctg ctttcacagg atcagcatct acaccatacc
      481 ctttaagata gtagtaagcc aaataggttt gtgcgttgac atgtccatga ttggctgctt
      541 gtgaaaacca gtaaaaagcg gctttatcat ccgcactaat cccaccttcc tggcctgtat
      601 acaatagacc tagactgtat tcgccctttt catccccctg ctctgccgct ttttgatacc
      661 attcaaatgc tttttttaca tcttttggag ttccttttcc catcatgtac tgataaccca
      721 aattcacttg tgccttgcta tagccttgtt ctgccgcctt tttaaaccag gtgaatgctt
      781 tgtcatcact ggcagctaca ccatctcctg tggcatacat taatccgata tttctttgtg
      841 ctataggatt tccttgctct gctgccttca tataccattt gaaggcctca gcaaaatcct
      901 gagatacccc cttacctgta tcgtacataa atcccaaact taattgagcc aatgcattat
      961 tcttatcagc tgccttttga taccaatttc tggcttcttc gtagttagtt gtcacaccat
     1021 atccgtattg atacatgcgt cccaataaat acatggcttc ctcattacct tctttggcag
     1081 cctgcattaa atgtggatag gctgtagtgt agtcgccatt ttcaaatgct gcaaaaccaa
     1141 ctaactcatc ggcccaaaca gcagtgttaa tcgacaataa tagagctaac aaaaaacctc
     1201 gcatacttgc tccattttat ttccttttaa tttaagcgta gaataaagaa gcaacaaagc
     1261 aagtaattgt tcggttaacg cgtggaaaaa tttaaaaaaa tctcaatcct gaagaaaaaa
     1321 taattcaatt agttaacagt tttttcttta ggcatagtca aagataataa tatccataat
     1381 ttgttaaagt tttttggaaa gggaaataaa cccctgagtg atttacattt aaaaatccca
     1441 agagaaccaa ctgatcccct tgattaacaa ttttcttccc gtatttataa aataaacacg
     1501 ggaagcatta caattcttta aattacaata gtgtatggaa tttaaatctg gattatatct
     1561 tctataacga ttcaaatatt gcagcagccc ccattccagt tccaatacac atggttacca
     1621 taccgtagcg cccttttgtt cttctcaatc catgtagtaa tgtagctgtt cttatggtgc
     1681 ccgttgctcc taagggatgt cccaaagcaa tagccccacc taaagggtta actttctctc
     1741 tgggtaagtc aaggtcttta ataaccgcca aagcttgggc agcaaaagct tcattcaatt
     1801 caatccaatc caattgatct aaagtaattc cagcacgctt tagcgccata ggaattgcct
     1861 ttactggtcc gattcccatg atttcaggcg gtactcccgc aacggcatag ctcattaaac
     1921 ggcctaaagg ttttaaatca tactgtttta aagccttctc actggctagt aatgccactc
     1981 cagctccgtc actcgtctga gaactatttc ctgcagtcac tgtcccgttt aaagcaaata
     2041 ctggttttaa tttagaaatc gcttcgtaag atgtgtctgc tctagggcct tcgtccttat
     2101 ttatcaattt tttctttatt tcaacctttg aagttttcag atcagggtat cttatagata
     2161 tttcgattgg acttatttca gcgttgaaat cgagtctttc ctgcgcagca attgcttttt
     2221 tatggctttc tatggcgaat tcatcctgtt gttctcgagt aatttcccag cgcttggcaa
     2281 cgttttctgc tgtaatcccc atgccataag ctatagcaac gtgctcatca ttaaaaatag
     2341 atggattcgc cgtgtattta tttccaccca taggtaccat ggacatgctt tcgacaccgc
     2401 cacctagtgc taaatgagcc tcctgattac ggattgtatc tgcagcagta actatacttt
     2461 gtatgccaga tgagcaaaaa cgattaatag tcattgctgg aacagattgt ggaagatcag
     2521 ccaataaaga agcgattctt gctacattca taccttgttc cgcttctggc attgcacaac
     2581 ctattactac gtcagctatc tcttgccaat caatgttttg atgtctatgt attaaacatt
     2641 taatagtatg agccaataaa tcatcgggta atgtgttttt aaaaacacct cttggtgctt
     2701 tacctactgg tgtacgtaat acgtcaacta catatacgtc agtcatccta attctccttg
     2761 cttagttgcg cagtggttta ccagtctcta gcaggtgact gattctggct tgggttaatg
     2821 atgtattggc taattttatg aatgcttctc tttccaactt tagcatccat tgttcatcaa
     2881 ccaatgttcc atgattcaca tccccaccac acaacacttt cgccaattca ttcgccagaa
     2941 aataatcatg ctgtgatata aatccacctt ccaaccaatt cactaaaccg gattgtaatc
     3001 gggcatgccc ctctatacct gccactttaa atcgttgttt tttcatagga ggaagataat
     3061 tagcagctcg catggctttg attttttcta aagaggcata taaaacctca tcagcatgca
     3121 ttagaataga gtcagccgcg cgcagatatc ccatatttct ggcttccacc gcacttgcag
     3181 ctacttttgc agttgctatt tgctgaaaaa ctggcaatat aaaagttaat aaatcagctt
     3241 gttgggcatg atgtgctgaa cgcatagcga gctctttgca accaccacca gcaggtataa
     3301 ctcctactcc agcctcaact aaaccgggat aagattcaaa agcagcaaca atagaatcac
     3361 aatgcatcat taattcacaa ccacctccta atgctcgacc tcttaaagca gctacagttg
     3421 gaattgaact atattttaag cgcattgcta cccgttgaaa ttgctctacc atatgctcaa
     3481 gagcttgtat tgttccatct gttaataatt tggagacgcc acgtagatca gctcctgaac
     3541 taaaattgga tgcatcttgt tgataaataa ttaatccctg gtaatccctt tctgcgatat
     3601 ccaaagcagt ctctatgcca tcgagaactg attgaccaat tgtattggct ttgcttttaa
     3661 aactaatcac agccacatca tcttttaagt accacaaaca aacaccctca ttttcataaa
     3721 taacatgaac aggatgatgt gactccttta ataccctgtc atggaagaat tgtttatggt
     3781 aaacaggtaa ctgacttctg gcgtgataat tgtttttacc aggcgaataa gcgccttctc
     3841 gtgtgtaaaa ttcctttatt tcatataacc actcaggtag ttttgcgctt gataatgccg
     3901 tattatcctg tattgattca tcgatttgtc gagtcacact gtgtaaatca gaaagctgcc
     3961 aggtttcaaa tggtccttgt gaccatccaa atccccagcg aattgccaga tcaacatctc
     4021 ttgcattatc agcaatttct tccaaatgat acgcacagta atggaataaa tctctaaagc
     4081 aagccgccaa aaattgagct tgcttattat ttgatgtcat taaattcttc attcgagctt
     4141 caggatcttt aatatccatt atcgctttca gttcatcact cacttctgct cgagagggtc
     4201 tatattgacc ggtttgaaca tcataaactt ctattacttt acctgctttt cggtaaattc
     4261 cttggccaga tttttgccct aaatgacctt ccttaattaa accattcaac cagtctggca
     4321 gaataaacat tttatgccat ggatcagcag ttaattgaga ctgcatggta ttaataacgt
     4381 gttgcatagt atccaaacca accacatcca tggtacgaaa agttgcagat ttcggccttc
     4441 ctaataatgg accagtcaag gcatccactt catcaattcc catattcatg gctaaagtat
     4501 gatgcaaggt agttaataat gaaaagactc ctattcgatt tgcgataaaa ttgggagtgt
     4561 ctttcgctct tacaactcct ttgcccaaat actcggttaa ccatgtttcc aaataatcga
     4621 gcaaattaga tgatgtgcta ttggcaggaa ttagttctgc caaatgcatg taacgcggtg
     4681 gattaaaaaa atggactcca caaaaattgg atctatgctg cgcaggtaat acctcacata
     4741 gcttattgat acttagccct gatgtattac taactaaaat tgttttatca gataaataag
     4801 gcgaaattcg cttgtataag tcctctttcc aatctatacg ttccgctatc gcctctatga
     4861 ctaaatcaca agaagataac tcttccaaat tttccgcata attttttgcc tgtaataact
     4921 cattggtttg gggagttgca agaggagttg gttttagttt acctaaattg gcaatagctt
     4981 tatcgaccaa gctgttttta ggcccttctt tgccagctaa atcaaacagt aaagtctcta
     5041 ttccggcatt aacgcagtgt gctgcaattt gagcacccat gacgccagcc cctaaaaccg
     5101 caactttttt aatgaaaaaa ggctcctgca ttgttattat cctcttgtta ataatcgatt
     5161 gatgcctcta tgagtgagat ttacaggtaa caggcactta ccattggcta aaaggagcaa
     5221 taatctgttg atcttgctcc tttctacatc ttaacttaaa ttaaaacgaa taccctgagc
     5281 caaaggcaat tcatctcccc aattaattgt attggtttgt ctcctcatat aagctttcca
     5341 gctatctgaa cctgattcac gtccgccacc agtttccttt tcgcctccga aagcacctcc
     5401 aatttcagcc cctgaagtgc caatattaat attggcaata ccacaatcac ttccacaagc
     5461 actgaggaag agctctgcat tttttaagtt ttgggtgaat aaggctgaag aaagcccttg
     5521 aggaacacca ttatgtaaag caatagcctc atccagtgtc cgataggaca taacatatag
     5581 aataggagca aaagtctctt cctgcacaat atcccaatcg ttttttacat cacaaactaa
     5641 agtaggctgc acaaaggagc cagcttgttt taatatttct ccaccataaa caatttgacc
     5701 tcctgccgct ttgattctat ttattgcttt tttaaactgt tcaacagctt gctggtcaat
     5761 caaaggaccc attaaatttc gtgtatcgag tgggtcacca atagttatct gttcataagc
     5821 atgtcttagc cttttgataa catcctggta ttttgattca tgaacaaaca ggcgtctcgt
     5881 agtagtacaa cgttggccag cagtacccac agcaccgaat actatggcag gaatagccaa
     5941 gttcaaatca gcagactcat cgaggatgat gccgttatta cctcccaact ccagaatgct
     6001 tttacccagt ctggcagcga cttttgctgc tacttgcttt ccaactgcag tagagcctgt
     6061 aaaggatatt aactgtattc gtttatcatc aaccatggct tcaaccacat cgtgagaatt
     6121 gggaatgatc aagccaaata tttcaggaca attattttct ttcaatacct gattgcaaat
     6181 atgttgtact gccactgcac agagaggtgt ttttgccgat ggcttccata tagtaacgtt
     6241 gccacaaata gcagataaaa atgcattcca tgaccataca gcaacaggaa agttaaatgc
     6301 tgaaatcacc ccaactattc cgtacggatg ccattgctca tacattcggt gatttgggcg
     6361 ttctgagtgc atggaattac catacaacat acgcgattgc cctacagcaa aatcagcgat
     6421 atcaatcatt tcctgaactt caccatcacc ttcctgtttg gatttaccca tttccagcga
     6481 taccagactt cccaagctat ctttattttc tcttagtgct tgccctattt gcctgatgat
     6541 ttcaccacgc tttggagctg gcacttttcg ccaggcttgc gctgcttgct ccgctcgctg
     6601 cataacctgc tcataatcat ccatagtgca agttgctatt tcagcaagtt tattcccagt
     6661 actgggattg aaggattcca gagtatgggc atggttgtca ctatgccaac catgtccact
     6721 aaaggctccg gggtttactg atttaatatt taaacgttgc aataaatcca taagattctc
     6781 ctaggcataa tacttgccga agcgattttc cagcacgtgg gacaatttaa acttttcttg
     6841 caaaataagc cctttatatt catttccctg tcccaatacc aaatcaacaa cagcgcatac
     6901 gcccgaagct gtactgactt gaatagctga ccactccaaa cctcgaatta cttcagggta
     6961 aattttcttg acataacttt tttcagttag ttcgccctgc ttaataccct caacagtaac
     7021 ataaactatc acgatatcct gatatgtttt tggaattgca ttttccagta ttctttttaa
     7081 cgtccctcga tcttcattta atcgtaagtc attcattaaa aaacgcattt ttttacaatg
     7141 gcctggataa cgcatggttt tgtaattcat cgattgtatt tttccagcat aaagttcagc
     7201 caaacttccc aaaccaccgg acgtgttgaa cgcttcatat tcacaaccat caatctgaat
     7261 tgactccaat ccttccagtg gagccataac cacatcttta ccaccttcaa taccataaca
     7321 aggattacca tattcattaa taaccccatc agtagaccag gttaatgaat aatgaagcgc
     7381 attattagca cgctgcggca atgctccaac tcttagtctg gcatgatgac atttctcaaa
     7441 ttcctgcatc aaactattgg ccgcaatact gataaagcca ggagctaatc cacattgagg
     7501 aacaaaagct gtctcggcat tttgagcaat agcttttact gcctccgtta cagaggtatc
     7561 ttccgttaaa tcaaaataat gagctttggc agctttggca gctttggcaa catgagtatt
     7621 taaaaaatag ggaagactcg aaatcactgc gataatatta tgcttttgca aataggcctg
     7681 agtggattgt tcatctttaa catccaatgc aaccgtttta atctcaggca gcgcagttaa
     7741 taatcttgtc acatcagagc cattaaattc cagatcggcc aaatgaacct gataagaccc
     7801 actatcggcg agtagacaag caattagact gcctattttc ccagcaccag taatcattac
     7861 gttgtacata caatccttct cctttgtaaa acttgataat cctacacaaa ttatcagaga
     7921 ttagatagat ccaaaacatg atcaatacag caattcttga tatcacttga ccaattagtc
     7981 ttactcattt atcttgacca ctttaatcag tctagttgtg acatgaagca ggccgctttg
     8041 atacattcga caataataaa cacatcaaca ctaaaccaat attcgaagtt cattgttgta
     8101 tttttagcag gtttattagc ccctttggga tttgcaccgt tccatctgcc aggattaact
     8161 attttgagtc tggccatttt attttctaaa tttcttcaat gcacggttaa gcagggattt
     8221 actcttggtt tcctatatgg gttaggatac tttggcttcg gagtatcatg ggtgattatc
     8281 agcatccacg actatggaca acttgattac tttcttgctg gagttatcac cttattgttt
     8341 atcatgtatc tttctctgtt tcctggaata gtcggttatt cctttaaact cctttcagcc
     8401 aagtgtaatt tattaacatg catgcttttg tttagcactc tttggtgtat aggtgagctc
     8461 atccgttcca agctttttac tggattcccc tggctattaa caggtataac ccaaatcgat
     8521 acaccattga gatatatggc accagtagtt ggtgtttatg ggcttggatt aatatgttct
     8581 tttttgggta ccctactggt tcttataatt aaagacaata cgattaaacg atattactac
     8641 cttagcgttt ttaccttgat tcttattgca ccatccctgg ggaaaaatat ccattggaca
     8701 gaaatcaaga aagaacctat ttctgttggt gcaatccagc ctaatctctc aatgagagac
     8761 aagtgggacg aaactctctt ttgggattta ttaaaatact atgaaaacaa aacagataaa
     8821 ctgttaggaa aaaatttaat tgttttaccc gaatcagcca tacccctacc agcaagttat
     8881 gttgaagatt acctacagaa actgcatgaa aaagcattaa aagccaaaag cagtttaatt
     8941 ttgggaatct tgcaacctac agataatgat gaaaccaatt tttacaactc tatcattagc
     9001 ttgggggaag ctacaggggc ctatgtgaaa cgccaattgg taccatttgg agaatatata
     9061 ccaaaaccct ttatccccat caatcgttgg ttaaatcttc cagaacctaa tgttgttccc
     9121 ggcagatcga atcagccatt aattaacatc gctaatcatc ccattgccag tctaatttgc
     9181 tatgaaattg cttacccaaa tatcttgaga tcacaaatgc ctgcagctca atggatagtc
     9241 tctatcagtg ataatggctg gtttggccat tccctggcaa gctaccagca attacaaatg
     9301 gctcaaatgc tctctttgct tacaggacga taccaaatcc tggtgaataa tgatggactc
     9361 tcatcagtaa ttaaccacca aggaactatt gtcgaagggc taccagcttt taattcagga
     9421 atgctccaaa cagatatttt tcctgcaaat ggcatcactc cctggataat ttggagtgac
     9481 tatcctgcct ttattttttg tttcatattt gttctttttg ttttgttttt taatattaaa
     9541 cgatttacag aaaaataatc tattgctggc aaaggcaaga ggggttatcc ttagcagttc
     9601 cgtgttatta aatttattgg cctatggata atacctataa cccccaagaa gttgaagaac
     9661 aagcacaaca atattggcat aagaagcaat catttaatgt tactgaagat ttaaacaaag
     9721 aaaagtttta ttgcttatcc atgtttccct atcccagtgg cacattgcat atggggcatg
     9781 tgagaaacta tactctcggt gatgtcatag cccgatatca gcgggcattg ggaaaaaatg
     9841 tcctgcaacc tataggatgg gatgcttttg gcttaccagc cgaaaatgca gccatcaaga
     9901 ataaaattcc tcctgcagaa tggaccagga aaaatattgc tgccatgaag gagcagtttt
     9961 tacgcttggg aaatgcttat gactggaaaa gagaacttac tacctgtgat cctgaatatt
    10021 atcgctggga gcaatggttt tttattcgtt tatttgaaaa gggcttagtt tacaagaaaa
    10081 acgctgttgt taactgggat cctgttgatc aaacagtatt agctaatgaa caggtagttg
    10141 acggtcgtgg ctggcgttca ggtgctttgg ttgagcgcaa ggaaatttct cagtggttta
    10201 ttaaaattac ctcctatgct gatgagcttt taagctcttt agatagcctg gatgaatggc
    10261 ctgcacaagt aaaacaaatg cagcgcaatt ggattgggaa atccataggc acagaaattt
    10321 acttcaatgt aaacaattat cccaaacgat tgaaaattta cactacccgc ccagatacat
    10381 tgatgggagc aacctatctt gctgtggcaa cagatcatcc attagcaaaa gaagcagctt
    10441 ccaacaataa aaaggttcaa gaattcctcg atagttgtca aggaattaag atagctgaag
    10501 cagaactggc tactatggaa aaacgaggga ttgacactgg aatgactgca atccacccta
    10561 taacgggtaa ggagttaccc atatgggttg ctaattttgt tttaatgcaa tatggttctg
    10621 gtgcagtcat ggcagttcct gcacatgatc agcgcgattg ggagtttgca caaaaatacc
    10681 aattgcctgt caaacaggta atcaagccta tagacattga gcatgacttt aatcaatcag
    10741 cttatactga agaaggaatt ctgattaact caaatcagtt tgataatcta ctctctagta
    10801 aagccattca agtcatcaca aactttctgg aagaaaatga cgctggcaaa gctaccatta
    10861 attaccgttt aagagattgg ggcgtttcca gacaaagata ctggggaacc cctatcccca
    10921 tgatcatctg tgaacaatgc ggtattgttc cagtccctga tgaagaatta cctgttgttc
    10981 tgccagagaa tgttgatttt accggtaccg gctctccact tacacaatgc aaagagtttg
    11041 ttaatgtaac atgtccaaaa tgcggtcaag atgcaactcg agaaacagat acatttgata
    11101 cctttgtcga atcttcatgg tactatgctc gctttgcttg caaaggtcag gaaaatgcca
    11161 tgcttgatga ccgagctaaa tattggacac ctgtagacca atacatcggt ggaattgaac
    11221 atgcagtgat gcatctgcta tatgctcgat tttttcataa attgatgcgc gatgaaggat
    11281 tagttaattc ggacgaaccc tttaaggctt tattaactca aggtatggtt ttaaaagacg
    11341 ggcataaaat gtcaaaatca ttaggtaatg tggttgatcc aaaccatctt atcaatacct
    11401 acggagctga taccgctcgc ttatttgtca tgtttgcatc ccctcccgag caatcactgg
    11461 aatggtcaga ttctggagtc gaaggagcac accgtttttt gaaacgggtt tgggcgtttt
    11521 cacatcagca tagagacatg ctcattgata ttaacgatag tattttgagt ggaaatgggc
    11581 atgtcgactg gaaggaagcg gagtctcgat taaaaaaatc ccgtcatatt gttcatcaaa
    11641 tacttgctca ggcaacccat gactatgacc ggaatcaatt taacacggtt gtttctggct
    11701 gtatgaaatt atttaatgaa atatctgatt actccattga aactgaaaac gataagtttt
    11761 ttatacactc aagcatcagt atattgctga ggctactcgc accaattacc ccgcatattt
    11821 gtcattgcct ttggcagcaa ttaggttttg ataaagcaat aattgatgcg ccgtggccaa
    11881 aggtggataa aagtgctcta aaaactgatg aagtagatta tgttgtacag gttaatggaa
    11941 agctcagagc tcaatttact gcaagcactg atgcgactga agaggaatta attgctgcag
    12001 ctaaggaaca tgcccacaac tttgtggtaa accacaccat taaaaaagca attattgtcc
    12061 cacataggca actaatcaac ttggtcattg gataaacatg tgtgattcgt cccagcaatc
    12121 tctaaatatt ttacacaatt tctcaagtta tgggtggcat tacctgtaag tgcaaataag
    12181 agagggaaaa gaagaatgac tcagatccac aagtacttag catctcttat actgatttgg
    12241 ctgctaactg gttgtggttt tcatttgcgt ggagcaattg atatccctga gtggcttgat
    12301 aatgtttcta ttatttccag ggatggaaat aaagaactgg tttctatact caaatcccag
    12361 ctggaaggct ataaaattga tgtcaactct gacccatctc tcgcgaaata ctggttaatt
    12421 attaacagtt ccagtttaca gcagcaaatt gttagcattg gcgcgagtac taatccaaga
    12481 caatatcaat taatcttaac cattgaattt atgttgcaga ctcccaaagg gcaaattatt
    12541 aaaaccccca gaaaagttaa tgtgagccgt caactcaccg tgaataatga caggatatta
    12601 gggtcaaatg aagaacaagc tcttttagtc agcgaaatga ggcgtgatgc cgctattcaa
    12661 atcattaatc gtttaaaccg accataatat gcaaataaat caacaagctc ttactcagct
    12721 tttacaaaaa aaaatacatc atcttaatat actaattgga caagatcatt atcttattga
    12781 cgaagcactc aataacataa aatcaatgct taaaaaatcg tctgaatgtg atgagaaaat
    12841 cctctctatt cagtcccctg aagaatggca tgtcgttctg gaagaaacaa ataattactc
    12901 tcttttttat caaacagtta ttttaaccat tttctttgat aaaaaaacga tagatacaac
    12961 gggaaaaaaa attcttagtg aatatctcaa atcagccaac tctcatagtt atattattat
    13021 cagagcccct aaccttaccg ccaagcaact tcaatggtta acacatgatg aacaagcgct
    13081 gattatcatt gcacaaccat taagctcaga agcaatgaag agatggatta atgagcaatt
    13141 aaaattaaat actatctctt ttgatccata tgttcctgaa ttaatttatc aatatactaa
    13201 aggaaatatg ctagctaccg ctcaggtaat tgaaaaaatt actttgagca atacaccccg
    13261 cactctcatt actccttacc tggccttgga acatttatcg gatcaatgtg atttttccct
    13321 ttttgaactg attgattcat gcctacttgg tcatagcgac agagcaattc atattttaag
    13381 acaagctgcc aataataaaa cagaagtcac attggtatta tggatactta cccaagaaat
    13441 aagaacgcta ttacaattat tatttctaat gcaacaaaag atggatatta aaatggcatc
    13501 taatcaatta aaaatttgga ctcaaagagt taatctttat caatcagctg ttaaaagatt
    13561 aaatacttta cttttacaac aacttcatca ctattgccaa tctattgatg ataaaatcaa
    13621 atccaactca aacaatcaag catggaatgc tctagagaac atttctcttt ccttatgttt
    13681 agggcaatta ctaggtgatg catgcatagc atagcaatat ttggtggaac atttgaccca
    13741 gtgcataatg gccatatcaa gaccagcttg gcaattcaag ctaactttgg ctttgattcc
    13801 tattattttt taccttgtaa aagccccgca atcaaacctc cgtcctttgc cagcagtgaa
    13861 caaagagtag aaatgctaaa gttagcactc aaaccttacc ctgattttaa aatagatact
    13921 cgagaactgg atcgagatac cccctcttat atggtataca ctttgcaaag tttcagacag
    13981 gaatataccg attcatcatt aactctaatt attggatacg atggattatt aaatctacca
    14041 caatggtacc agtgggaaaa aataatatcc ttagccaatt tacttgttat caatagagaa
    14101 gagttcttcc aacagcctgt gccaaaatca gtgcaaactc tacttaacca atatagaaat
    14161 gatgataaaa atatactgct aaatcatcat gctggtagta tctgcctata caatgctgga
    14221 cactatgata tatcctcaac aaaaattaga gaacaattaa aacaacataa agatgtaaag
    14281 aataatttgc ctgatttggt atacaattac attaagaagc agggactgta tcaataatat
    14341 tttctgtttt atcaacagcc ttgacctgct ttataaattc aggcaaatgg ctatatagca
    14401 aagctacaac aggatcaaat cgtgcataaa gtttggactt ttgtgaatat tcctgataag
    14461 gcaaagaggt cattttaaca gcaaccataa tcagggcaac gataaaaacg ccacgtaaaa
    14521 agccaaaacc cattcctaaa gttcgatcag ttccgcttaa accagcccgt tttaagataa
    14581 agctgagtat tgcattcact acccccccaa caaaaagggt ggcaaaaaga ataataataa
    14641 atccaatagc cgttcgtact gactgttcct cgatataaga acttaaccag ggatccaggc
    14701 tttgtgaata attaaaacct agccaaatag ctaatatcca cacacataat gcaacaagtt
    14761 cctttacaaa accacgaaac aatccggtca atacggacaa agcgataatt gcaacaattg
    14821 ctatatcaac ccattgtagt tgcataacta actaactcca gtattcacaa caaaaccatt
    14881 taattgcatg gcactagcta attgagtttt taattttatt acatcttttt taacgggtga
    14941 atggccaaca actactttat aaactattcc atttctgcta ttagatttaa gataattcgc
    15001 cttgtagcct ttgctgcgca atcgattaac taaagcttga gcattagcta tttgtgtaaa
    15061 tgaagccagc tgaacagcat aaatatcttg tttaactgct ggttttctcg cagcaacaat
    15121 ttggctgacc tttttgttag tattaacctt tgccgtaaca actggagatg ttttcaccgg
    15181 tggttttgct gcagccaggg tcattacttt ctttgctgtc gcattcgctg ctttatttac
    15241 tgccaattta attggctctg atttaattga gttctctctt tccgcttcaa gtgtggcgac
    15301 tttagacgtt ctgatgagat tgtcttcttc tgtgctgatg ggttctgctt ttgccaggtt
    15361 aattgatttt ttttcaactg gtattggggg tatttctgtc ttagccactt ttatagtttt
    15421 aaatacctct ttttcatcag taatcactac gtcaggaggt aatggttttg gtggtaattt
    15481 cacattgaca ctaaagttac tttcagggct ttggctggat ttttttatta gtgcaggagc
    15541 aaaaattgcc cccaatgaaa taataacggc caaacctatc aagcgatgtt taactttctc
    15601 atccattatc agtttcattg attctccttc tgctccaaaa gattatgttg ggcagccatt
    15661 acatgactaa ccgtaaaaaa tgaaccataa acaataatta aatcgtcatc tgatgcttgc
    15721 tttaatgcca tttcaaaagc tgtaaatggg tcattataac aaacatctag aaaaatttct
    15781 gcttccctga atacagaaag caattcctct tgacttgcag cgcgcttatt gtgtaattgt
    15841 gcaggatacc atcgattaac acaatccctt aaaggcataa tcaatccata aagatcttta
    15901 tcttttaaag cggagaaaac agcatgaatt tgccctttta cattgatttt ttttattgta
    15961 tcagcaagta atcgaactga ctgcggatta tgagatacat cgtacaaaat atcagtcttt
    16021 cctttttgta attgcaaccg ccccgggata taaatgagct ccatcgcttt ctttagattc
    16081 ttataggata caggcaaatc attttgcatc aaatgacaag ccattacagc tgctgccgca
    16141 gaacgaagtt gaatttttgg ccgggtcaat tgctcaaatg gcttaccttg acaagtaacc
    16201 ccccaatggt cagcttcctc ctgataaaaa tagtctttac cgtataaata agatgatgct
    16261 ccaagttgta atgcaacctc aacgacactt tttggtggtg agagatccgc gtagataaaa
    16321 ggtttatttt ttcgtagtat tccggctttc tcatacccta tcaactcaag tgtgtcacca
    16381 agaaaattct gatgatcaaa gtcgatagta gtaatgatcg ctaaatcagt atcaacaatg
    16441 tttgtagcat ccaaccgtcc tcccaagcct acttcaagaa ttatcagatc cagatcatgc
    16501 tgtttgaaat accataaggc agcaagtgtt gccatttcaa aataggttaa attggtaggg
    16561 taacgtgctt cttcaatcac agaaaaagcc ttacataaat ctttatcttg aatcggtgtt
    16621 aaattaattc gaatacgttc attaaacaca agaagatggg gcgacgtata ggtaccaact
    16681 ctaaaacctg actggtgata gatggtttca agtgctgtga ctgtagaacc tttaccgttt
    16741 gttcctgcaa ctgttattac cttgcaattt ggtcgctgca aatttaattt ctgcgctaca
    16801 gtcaaaactc tggtcaaacc aagctgtatt tcctgggtat ttctggtttc caaatgagtc
    16861 aaccagtgat ttaaatccca ttcactccat ggcgtgtgca aaaaaactct ccgctccttt
    16921 atgagtcaat ttggaaacca attcggatat agttgctcga agatgtttac gttcaactat
    16981 catatcaata tgcccatgtt ccagtaaaaa ctcacttcgt tgaaagcctt ctggcaaagt
    17041 ttgtctaacc gtttgctcaa tcactcttgg cccagcaaaa ccaattaaag cattgggttc
    17101 agcaatgatt atatcaccaa gactgccaaa actagctgat actccaccca tcgttggatc
    17161 ggttaacaca acaataaaag gcaacccggt ttcagcaaat tttgctaacg ctgctgatgt
    17221 tttagccatt tgcattaaag aaaataatcc ctcctgcata cgagcaccgc cgctggcagt
    17281 aaaacaaatg tatggtcgct tttctgttgc agcaacattc actgccctta caaatttttc
    17341 tccaaccgtc gctcccatgg agccgcccat aaaattaaac tcaaaggcac tgacaaccac
    17401 cggttgttgc attaaaaacc ctttaacaac aattaatgcc tctttctctc ctgtcgcttt
    17461 ttgcgcttga gaaattctat ccttgtattt tttggagtcc ctgaatttta atctgtcctg
    17521 tggctccagc ccagcggcca cttcttcttg ccctgactca tctagaaatt gagcgattct
    17581 caagcgcgca gaaatgcgat gatgaaaatt acaagatggg caaaccataa aattttttgc
    17641 aagttcggta ctgtaaagca cctcattaca accagaacat ttaacccata aaccttccgg
    17701 aacccccttc ttttgagagg tttctgtcct tattcttgat ggtaacagtt ttttaagcca
    17761 gctcataaat tagaacccag atattatcaa atcggattat tatacacaga aaaaaataat
    17821 ctgacatgtc tatattgggc ttgaatttca cataaaggaa taagatattt tatcaaatcg
    17881 acttgataac ccgaaccaaa tcaccctgaa aaccccaatt actctttaaa aatattcaag
    17941 tccatctgag gaattttaga cacgttgatt atattgcaat cgatctttct atttatttaa
    18001 caccctaatt ttattcaata attttttgcg acttttcaaa aaagcacttt cggaactaaa
    18061 gtttttttca ctatttccga taactaaatc gggtacagta aattcacaac aataaatcga
    18121 atttactgaa ttacaaatgt cccataccag ggattcaggt ctgaggagac taaaactatg
    18181 gctcaagtaa tcaacactaa tgtggcttcg cttacagccc aacgcaattt gggtgtttcg
    18241 ggcaacatga tgcagacatc aatccagcgt ttatcatcag gattaagaat taacagtgcc
    18301 aaagatgatg cagcaggact cgccatttct caacgaatga ctgcacaaat tcgcgggatg
    18361 aatcaagccg ttagaaatgc taacgatggt atctccctat cacaggttgc tgaaggagca
    18421 atgcaggaaa caaccaatat tttgcaacgt atgcgtgagc tttccgttca agctgccaac
    18481 tcgaccaata actcatctga ccgcgcctca attcaaagcg aaatttcaca attaaaaagt
    18541 gaattggagc gtatcgctca aaatactgaa ttcaatggtc aaagaattct cgatggttct
    18601 ttctctggcg caagctttca ggttggggcg aattcaaacc aaaccatcaa tttcagtatc
    18661 ggcagcataa aagcttcttc tattggtggt attgccacgg caacaggaac agaagtagct
    18721 ggtgcagcag cgacagatat tactatcgca attggaggag gagcagcaac cagcattaac
    18781 tcttctgcca attttaccgg ggcactaaac ggacaagatg ctacgtctgc ctatgccaaa
    18841 gcagcggcta tcaacgatgc cgggatagga ggactatcgg ttacagcctc tacgagcggc
    18901 acacaagcag ttggcgcaat aggtggaacc gcaggtgata cctacaactt aacgattaat
    18961 ggtgttgcta tatacactaa ccttgatgta gcaacagcat taaccaactc cgatcttcgg
    19021 gatgccatca atggtgtcag taaccaaact ggagttgtcg cgtcacttaa cggtggtaac
    19081 atgacactca ctgctgctga tggcagaaac attacggtaa cagaaagcgg tacaggtttt
    19141 actgcaggta ctgacggttt aactgtgact ggcggcgcat ttgatggcgc gctacgcggt
    19201 actttatcaa tcagcgccgt tgataccatc gcgattggtg gtactgttgc taacattggt
    19261 ttaagtgcca atatctctaa agacacagta gggatagatt cactggatgt gagcacagcg
    19321 tctggcgctc aaacagcgat taagagaatt gatgcagctc tcaatagcgt aaactctaat
    19381 cgggctaata tgggtgctct gcaaaatcgc tttgaatcaa cgatagccaa cctgcaaaac
    19441 gtatcagaca acttatcagc tgctagaagt cgcattcaag atgctgatta cgctgctgaa
    19501 atggctagtt tgaccaaaaa ccaaattctg caacaagcag gtacagcgat gttggcacag
    19561 gctaatagcc taccgcaatc tgtattatca ttgttaggtc gataggatgt cgcaatcgag
    19621 gtgcagcaac aactgcactt cgatttcttt ataccgcggt tgatgggtat aaaaaggagt
    19681 atgaaaaatg agtattgaac ctgtaccctc agtcaatagg gcagctcaaa cagatcaaat
    19741 aaaaatagtt gaggaagcct ctaagccaga aattaaaaat atggtttatg attccgcttt
    19801 ggatgccgaa acaaaacagg cgctggataa agcaacaggt ttgcttcaaa ctataataac
    19861 agagaaaata tccgataaag tattacgtaa aatgccttct gatgagtatt tgcaattact
    19921 aagcttgctt gatgatatta ttagtggttc tatagacaag catgtttaat aatttgaaat
    19981 atttattaaa aatttgacta gaacaataac aacatagaaa agggatactc tatggaactc
    20041 tcttcaccag gcgtagggtc aggacttgat gtaaaaaacc tggtcgatgc gatagtcacg
    20101 gcagaaataa ctccatcgca agtgcgtcat gataaacagt taaacagtgt caatacagag
    20161 ttgtcggcgc ttggtcaatt aaaaagctat ttaactaact tgcaaacttc gttagccaag
    20221 ctctccagtc ttagccaatt ctacactatg aaatcaacca ttagtgatcc gaattacttt
    20281 tccgctactc tggggactgg cgcgactaaa aatacttatc aaattgaaat acaaaaactg
    20341 gcgcaacaac acagccttgc aagctcttac atgactaata ctggaagtgg aacaataacc
    20401 ataaattttg gaacctataa cagtgataaa accactttta cacctaatcc ttcagctacg
    20461 tcagtcaata taaccattgc gcctggcaat gatagccttg ttgcggtacg agatgccatt
    20521 aataatacta attcaggagt aacagccagc atagttcaag atagccaggg ctccaggcta
    20581 actattactt cgtcaaaaac tggcgaaaat tatgccatga aaattagtgg tggagttgcc
    20641 tcattgaatt atgatcctac catgggagtg aattcattaa ctgaaacagt agcggcacaa
    20701 aacagtactg ttaaaattaa cggattaact ttggttcaaa gttctaatca attgaatgag
    20761 gcaataactg gggtcaccat tgatctaaaa aaagcagagg ttgggaaagt tgttacctta
    20821 actgttgatg ataacaaaga tcagctcaca ggcttaatca acgagttcgt aaaacaatac
    20881 aatgatacca tgactttttt aaccaattta actggttata acagtgaaac caggcaagga
    20941 ggggtatttc agggcgatcc acaatttcgt aatttaaaac ttaatttaaa taagtgggca
    21001 actacccctt tggctaatca agaagggcca atccgctcat tggcagattt aggaataatc
    21061 accaataagc aaggattact ggaaatcaag caagataagt ataaagcagc acttgataat
    21121 cattataaag aaataggagc attgtttgca aaaacagcca aggctaatga tactggcgta
    21181 aacatcaaag ccattaattc aactgtcaaa tcaggtacct atgatgtggt gttgactgaa
    21241 tttactcctg gagtcagcat gtctggaaca ataggaggtt tatctgctac atcatctgat
    21301 ggcattacat tgacaggaag tggtgatttg agtggcctgt cgattaatgt attttcaggg
    21361 tctgccggag cccgtggaca aataactgtt acagacggta ttgcagtcca attaagttcc
    21421 ttgctggata cttacattga aattaaaggc gatttggacc agagaaaaga ccaactcaat
    21481 aatcaagtca agcaattgaa taaaatacaa aacgaaattg atatcagacg caaatcaatt
    21541 gaaactcgct atttaaaaca attcactgct ctggacactc tactcagcca attacaaagc
    21601 actagtgttt ttctatccca gcaattagcc aatttacccc aattaaaatt gaaataatag
    21661 gaggtaatga gaatgaaaaa cctatattca caaatttcta gccaatacaa agccatagag
    21721 ctacaaactc gcatagaaac ggcctcccct catgaactta tagatttatt actccaagga
    21781 gccagatctc acattgctac tgctcagggt aatatacagc gtaaccaaat taaagaaaaa
    21841 ggagaacaca ttggcaaagc cattagcatc attgaaggtt taaaaatgag tctcaatcat
    21901 gataaaggtg gcgaaattgc cgaaaattta ctccagttat atgattatgt tcaacaaata
    21961 ctacttaaag ccaatttaaa aaatgatgaa gatttattag ctcagtccaa tatgctatta
    22021 gctgaagtgc atcaagcctg gcaagcgatt aactctaatg ctggtaatga actttaatat
    22081 ttgcaatgag ctcccatgat tcagcatttg ctttttacgg ctttatagca aacaaaataa
    22141 gagttttctt gacttggcaa ttattaaacc cagtccatct atttttattc tcccaatgca
    22201 aaaagtttaa ggcttccaaa tatcaataag atatgaggaa ttgtctggaa gtcttgtagt
    22261 tatcgaattt tttgtacatt ttggtaatca agaataggat tataattttg gcaaatttgt
    22321 atagatttat cgaattggaa tataaagttt ttccattata tgccgccaaa acagttagga
    22381 tgaataaatt aatttcatta tgaatacatt taaatcatca ctggcatttg taatttgtct
    22441 cttacttggc atgcaatcgg cacaggcgca aatgcgatat ggagaaactc tctgcaactt
    22501 gcctgaatat ttttgcatga aaataaaaag cggggacact tgggataggc ttttccctaa
    22561 catcgaagag caagatattg tcagacgcat taaccgaatg aatatcaggc tcaaacccgg
    22621 gatgattatt gctgttccca aaaatataga gcgtttaacc atctatgatg tctcgccttt
    22681 tccaagatat attgaaccaa gtggtgaaaa aacaatttat gtcagtcaaa aaaaattggc
    22741 gtggggagct tatgatgaag acggtgaatt gctttggtgg ggaccaatat catcaggtgt
    22801 tggaaaatgt aaagtaatag gtggttgttc tacaccaaca ggttcatttc gcattatccg
    22861 caaacaagat attgattgtg tttcaactgt tttcccaaga agggcaaatg gcgaacatgg
    22921 tggtgcactg atgccttatt gcatgcactt ttttagaggt tatgctttgc atggaagtta
    22981 tgaagttcca ggatataggg ccagccacgg ctgtgttcgc atgtttattg aagatgcacg
    23041 ttggttaaat gaggaattta tcgacctccc agggggagga atgagaggaa ctcgtgtaat
    23101 ccttgatgcg ccagatgctc aagagaatcc cgataaccta caataatata ttgaaaacac
    23161 aatcaaaaaa ctcaccccca atagacaaat agtggtcaac ctgataatat tttatttagt
    23221 gaattttgcc atgaggtgtg ttaacgtata aattcactgt atgaataaaa taagtacatt
    23281 tttaatcaaa gattagaata tatggaaata ccatctatat cctctttttt gactaataac
    23341 tttcatagaa tctaatgatt ggataaattt ggagaatttt taaataatgg ctcattacaa
    23401 tgtacaatcc gaatcgctca ataaagcaag tgtcagctta attatgccct ggatggtgtg
    23461 gggcttgggt tgcttattct atttttatga atgcttatta caagtatccc caagcgtaat
    23521 gagtaatgaa ctcatgagag atttctcggt aacaagtcat actttaggga ttctatcagg
    23581 catttatttc tattcttatg ccgccatgca gcttcctggt ggtgttctaa tggattattt
    23641 tggcccacaa cgtttattaa ctctcgctac agtggtttgt gccgtgagta ctatagcctt
    23701 tggcatgaca gataatttct tcatggcctg tgtcgcaagg ctaatgattg gatttggatc
    23761 cgcatttgct gctgttggca ccatgaaact ggcagccaac tggtttccag cacaaaaatt
    23821 cgcattattg actggcttga tggttaccat aggcatgatg ggtgcaatag gtggagaaac
    23881 acctttggct cttctaattg ataattttgg ctggcgggaa agtatgatta taatgggtag
    23941 tgttggactt cttttggcaa ctttgctcat cttgattgcc agagatactc ccaagaattt
    24001 ttctcctgct atccatcaac atcctgtcga ggaagagcgt ttaatcccaa gtttattcac
    24061 attgataaaa aataaacaac tttggctggt tgctacctat ggcgggttaa tgtacatggc
    24121 aacacccgta ttttgtgggc tttggggggt tccttttctg atgaataaaa tgctaatagc
    24181 aaaatctaca gcagctaatt ttatctcatt ggtatttgtg ggttgggcaa tagccagtcc
    24241 tttatggggt ctgttttcaa accgtattgg attacgtaag ccgcctatgt atattggatg
    24301 tgtaggggca atgatttgtt ctttgctctt tatttttgct cccatcactt cttcaattac
    24361 tatggaaatc ctgctgtttg catttggtat cttttccgca ggctttttgc cagctttctc
    24421 agtagccaaa gaattatgta ataaaaaata tgtggcaaca gggcttagtt tcatgaatat
    24481 gatgaatatg gttggaatag cgttagcaca acctttaatt ggttatattc tggataaaat
    24541 gtggcaaggt caaatgatgg ggactgtgag agtttatcca ttggaagctt actacactgc
    24601 tttgtcaatc cttccaatag gcatgttagt tgcgttacta atattgccga aaattaaaga
    24661 aacctattgc caaagtgtta attaatgaaa taataccgca atttcaattt gtagaatcaa
    24721 aatggttaaa aaattatata ttaaaacaaa tggctgccaa atgaacgaat acgattcttc
    24781 taaaatggca gaagtacttt acgcttccca tggtttggtc aaaactgatc aagtcgagga
    24841 tgccgatgtt attttattaa acacatgctc aattcgagaa aaagcacagg aaaaagtttt
    24901 ttctcaactg ggtcaatggc gggaatataa agctaaaaac cctcatgtgc tcattggggt
    24961 gggaggatgc gtagcgagcc aggaaggctc agacatcata aaaagagcac cgtttgttga
    25021 tatcgtattt gggccacaaa cattgcacag gctccctgcc ctgctcaatg aaaggcttga
    25081 aaaaaataaa tccgttgttg atatcagctt tccagaaata gagaaatttg atcatttacc
    25141 cgcaccaaga gcagagggcc ctacagcttt tgtatctata atggaaggct gtagtaaata
    25201 ttgcagcttc tgtgtagtgc cctatacgcg tggcgaggaa atcagcagac catttgatga
    25261 tgtattggct gaatgttatc aattagccag tcaaggcgtc agagagatta atttactagg
    25321 ccaaaatgtg aacgattata gaggtattat ggataatggg gatatagccg atcttgccct
    25381 gttaattcat tatattgccg ctatcgatgg aataggaaga atacgtttca caacatcaca
    25441 tcccttggca ttttctgaaa atttgattaa tgcctacgct gaggttccag agcttgccaa
    25501 tcatttacac cttccggtac aaagcggatc cgatcgcatt ttatcgttaa tgaaacgagg
    25561 gtacactgct ttggaattta aatccaaaat tcgtaaatta aggaaaattc gaccagacat
    25621 tcgcttatct actgacatta ttgtaggctt tcccggagag acagacaaag atttccagga
    25681 cactatggat cttgtacatg aaatcggttt tgatacctct ttcagtttta tatacagccc
    25741 aagaccagga actcctgcag caaatttacc tgatgacacg cctatggaga tcaaaaagca
    25801 acgtttacag attttacaaa acagattatt aatgaatgca gctcgttaca gtgaatcaat
    25861 gataggcagc aagcaaaaaa ttctggttac cggattttcc aagaaaagtt cacagcaatt
    25921 atctggtcgt actgaatgta accgtgtagt taattttgat ggaccacctc atttaattgg
    25981 acaatttata gatgtccaaa ttagtgacgc actacctaat tcattgcgag gaaggctgct
    26041 tgaaaaggag atgcagccag cctaatgatt gagcatatcc agcaacaatt atcagtacct
    26101 cgcgaattac ataaccaacg agtagatagc gtgcttgcca ggctgctccc tgagtattca
    26161 cgctctctta tctgtaactg gattaaaaca ggtgctgtta cccttaataa tctgccttgt
    26221 aaacccaaag ataaagcgct gggcggtgat ttaattacga ttaatgttga acatacaata
    26281 gttaatacag attttaactt atgtaaacct gagaacattc caattcagat aatctatgaa
    26341 gacagtgaag taattgttgt taacaagcca gccggtcttg ttgtacatcc gggtgcggga
    26401 aacaaggaac atacactagt taatgcactg cttcatcatt gtcccgagtt gcaactactt
    26461 cctcgtgctg gaattattca cagattggat aaagatacca ctggtctgct gatagtggct
    26521 aaaaacctga ttagccatac ttctctcatc aggcaaatgc aaaatcgcga aattaagcgc
    26581 cactatctga cattagttca tggttatgtc atttctggtg gaacaataga tacgggtttt
    26641 ggcaggcatc ccaaaaatag actaaagatg gctgtaactg acttcgggcg tcaagctgtt
    26701 acccattata atatcaataa gcattataaa gactttacct tactgaatgt tagcttaatg
    26761 acaggacgga ctcatcagat aagagtgcat atgaactata ttaaccaccc ggttgtagga
    26821 gatcaactgt atggaggaag aatgaaattt cctcctaatg ctgatgaggc aatactctca
    26881 gttttaaaac aatttaaaag acaagcactt catgcctgtt ccatttcatt ttgtcatcct
    26941 gtaaaaaata cagaaataac actgaatgca ttattacccg atgatttcga actcttgtta
    27001 aacactctgg atcaacacga tgaaaaatag atttgccaat tggccagccc ctgaaaatat
    27061 taccgctttg agtactaccc gtttgtatgg tttcagtgac ggcccctatg caagcaacaa
    27121 ccttgggctg catgttggtg ataaccaaca gcatgtactc aaaaacagac aacagctcat
    27181 tgagcttatt gatctgcctg cagaaccaat ctggctggaa caaacacata gcacaaaatg
    27241 catcacggta gaaactgaaa aaaccagaga tgctgatgct gcaattactc gtttacctaa
    27301 actcccttta gtcattatga cagcagattg tctacccata ttactatgca atcgacaggg
    27361 aactgaaatt gccgccattc atgctggatg gaaagggatg tataatggta ttattgaaaa
    27421 tacattaaag aagctaaata gcaataggac agatataatt gcctggatag gccctgctat
    27481 ctgtaaaaaa tgttatgaag tgggtgagga agtttatcta tcttttacca ataaataccc
    27541 cttaactagg aatgcgtttg agcctgttca tagcaaatgg ttagctgatt tacctcaaat
    27601 tgcagaaata gtacttaatt tggaaggggt aaaatcagta tttcagtcaa atttgtgtac
    27661 ttttgagtta aaaaatgaat tctattccta tcgcagagaa tcacaaacag gtagaatagg
    27721 tactttcatc tggattaatg agtaaaatca ggattaataa tatgattaca cgtataaaat
    27781 tattcatagc aagtctgctg gtatttttca tcccggtggc cgatgcagca caagatctta
    27841 caaacatgcc cagcctggct cctgttctga aaaatgctat gccggctatt gttaacgtgg
    27901 ctgttcaagg ctacttacct aataatatgg catccggaaa tgcggacgat gatgacgggg
    27961 aaaacagtaa acagccttcc aggatacctg aaaaaggcag aaaatttgaa agtatagggt
    28021 ccggagttat cattgaccct aaaaatggca ttattataac gaatgaccat gttattcgta
    28081 acgccaattt aattactata actcttcaag atggcaggag attaaaagct cgcctgattg
    28141 gaggtgatag tgaaacggac ttggctgttt taaaaattga tgctaaaaat ttgaaatccc
    28201 tggtaattgg tgattccgat aagctggaag tcggagatta tgttgttgct attggaaacc
    28261 cctttggatt aaacagcttt ggaaatagtc aatctgctac gtttggaata gtgagtgcct
    28321 taaaacgcag cgatttaaat attgaaggtg ttgaaaactt tattcaaact gatgcagcta
    28381 tcaatcctgg taattcagga ggggctttgg tcaatgcaaa aggcgaatta attggcatta
    28441 atacagccat tatttcaccc tatggaggaa atgtaggtat tggttttgcg atcccaatta
    28501 atatggtaaa agatgtagcg cagcaaatca ttaaatttgg ctctattcat cgtggtttaa
    28561 tgggtatatt tgttcaacat ttaacaccag aacttgccca atcaatggga tatgccgaag
    28621 attttcaagg agctttagta tcacaggtca atgaaaattc acctgctcaa ttggctggtc
    28681 taaaatcagg cgatgtcatt gtacaaatta atgacaccaa gataactcag gcaacacagg
    28741 taaaaacaac tattagcctg ttgcgagccg gctctactgc taaaattaaa atcttgcggg
    28801 ataataagcc acttacatta gatgtagaag tcacagatat caaaaaacat gagcaaaaat
    28861 tacaatccaa taatccattt ctctacggat tagccttacg taattttgaa caggaatcac
    28921 cacctcatgg taatgttgtt ggtgttcagg tcgtaggtgc ttcggaaacc agtgcgggct
    28981 ggcgagctgg cttaagacca ggagatatta ttatttctgc taataaaaca ccggttaaag
    29041 acattaaatc tttacaagct gttgcacatg agaaaaagaa acagctatta gtccaggtgc
    29101 tgagaggagc aggagcactc tacctgttga tcatttaaat tttttaatcc caataatgaa
    29161 ataacgtatc ttctttcttt aagcccatct ctcagagatg ggcttaaatt ttgataaatt
    29221 atcctgcttt tgaaaatact gattatcaac tattccctcc catcctccaa aacaataccc
    29281 ggcttgggga aatatcactt ttcaatacat caacaaactt ctttttaaat acgctattct
    29341 actttgaaaa aatcatgcaa attcaattta atataaatta attataaaaa actcaggaaa
    29401 cagtcttgac ataaacacat gaaaaagaca aaatcacgac ctcttgtggc tatgtagctc
    29461 agccggttag agcgcggcac tcataatgct gaggtcggtg gttcgagtcc acccatagcc
    29521 accatttaca tcacatgcac cctgattgag aacacttgta gtgagcaacc aaaacctact
    29581 ccagattttg tatttcattt gtcacttcaa gcaataaaat agccactgta atcaaatcag
    29641 gccctgccca caaaaaatat ttgagatcat cttgcatcac gaaaagaaat tatagtagca
    29701 tcgttgctca tcacatttca taatacggat tttattttgt tcaggattaa cttaatacga
    29761 tatgtgtcac ttctgtttat tatcacattg aatttagatt gtattagcgt tgcattttca
    29821 tctcagatca tttatggaac acaaaatgct gcacgagtta ctgggaaaaa tctgaaaaga
    29881 atttattttc ttcagttagg cgcttttacc caaaaaaaca atgcccagca attggcaaga
    29941 aaaattcaag cgagaacaca ataccctgta ataattagtc attcaggcaa tgctttctcg
    30001 gttcgtgtgg ggcctgtcaa tacgcaagaa ctgcatcaac tcagcataaa actttcacaa
    30061 aatcccaata ttactccaat aaaacataat tcatcaccaa agcagatacc gactattcag
    30121 acaaaatcac aaatacttgt taaggaaact tctcgccctc ttccaactca ttctgtggct
    30181 aaagatacaa ttgatgggaa aggaagctgg tatgtaactg ctaaaattgg agcacaagaa
    30241 acaacaccag atgatcagtt tacagtaaat aacgacagtg gctttcccag cccttttgat
    30301 aaggatattt ataccaacag ttccgattcc tccttgctat taggcattgg tgggggatat
    30361 cgatttacaa atataattct cccagcggtt tctgttggaa tatactattc acatttcttt
    30421 aatatggaca ttaacggaca ggtgattcag tacagcttgc ccgaattcac caattacaat
    30481 tatcaatgga aaataaaatc tgatttggtt ttagcttctg taaaagccaa tgtctttcgc
    30541 tggaaaaatt taatgccatt tgtgcaagga ggcgttggag cgagctccaa tcgagccagt
    30601 caatattatg agaatgcctt gcctgatgtc acacctcgta tcagtcctaa ttttgccaat
    30661 catacggcta ctgtatttgc ttaccaactg ggcgctggcc ttgattggga gataagacca
    30721 caatggttgt tatctttagg gtatgaatat tctgatttag gcaaagtacg ttcaggttat
    30781 ggtactgata gctggaatgg agagacattg tcctcgaaaa atttgacaag caatgctgga
    30841 gtcttaggct ttacctattt atttcatgga taatcaaaag tgaggcacag gctaaaattc
    30901 caaaccttgg gaaaccacaa gttatttgtg gtttcccaac aataacttat caaatagaac
    30961 tgatttaatc ttcataaaaa taatgggtga aaggaccaat atgaaatact caactcgcat
    31021 cataatcacc acaatctgta tgttattcgc cttgctgggt tatgctggaa aagatcctat
    31081 cagctggcga gtcagtggag tatttcctgc tcaaagcagc gttggtcaga cttatactgt
    31141 gacttatacc tttaccaatc aattgccttt tcgattggcc aaggccttga ttatagaacg
    31201 atcggcaagc cccaacacgg aattcaattt taatgatcag tgttccggtc ggttattaaa
    31261 ttccggagaa tcctgtactg tcgttgtagc gttaaaccca cttgtaattg gtcttaaaac
    31321 acttcaattg actattgcag gatatagcaa ggatagagtt cctttacctc aaacaagcac
    31381 cttaagcaca ggacagtcct tgagtccaat tgttagcttt gtcatggatg gctttccctc
    31441 tcaattaacg acaggctcag tttccagtta taaatttacg tttaccaata acggggctat
    31501 ttcagctacc aatttatcag ttaatgtctc gcaatcatta ggagcaccta attttactac
    31561 gacgtgcaat aacacattga ctcccaacgg aggggtgtgt gaagtcgatg ggacgtacac
    31621 agtaacttcc gggagtccgt cgattcaaca agtcattgcc agtctgtctt atacaggccc
    31681 atcgggaagc ccggtaacgg cacagacatt cacacaagta gtcaatccag cagctccgct
    31741 tgtaggcact ttagtgccac cgcattatct accgccatta atggttgaaa acaccattta
    31801 tacagtccag tttttattta cagccagtgg aactgtgaca attgataatc ctggtggttc
    31861 aatagcttgc tcgcagggag ttaacccctg taatgttggt gttatgaaca taaccagctc
    31921 aagctgcacc gccccgcaaa cattgaccaa tgcggcctgt caaatgactg ctacgttcac
    31981 agcaccccct gcaacaatcc cccccgctgc cccacttact tacattctaa ctgcaacagt
    32041 tccctattcc gttggaggaa acccacaacc tgatgcaact gccatcactt ccgggacagt
    32101 acttaccgca ttaccgacag aaagaacagt gactttaatc aatgagtgcg attttgatgt
    32161 gtggttttcg ctgaatggaa gtcaattagg taattcaccc aattgcccaa caacaccatg
    32221 cccaaatggc acaagctgta ataccagtac gaacaaatgc ttttggaaca atccggcccc
    32281 aaacaacgga gtatatttat tacaagcact tcctggcact aatactaaca gtgtagctat
    32341 ccctgttacc aatgctgacc ccaatattca atggagtgga aatatttcgg caagcactct
    32401 gtgcaacggc acaacctgcc aacaagcggc ttgcgataat aatggcggta ctacctcctg
    32461 cgcgccaggc attggtttca aacaacccgc tacccaggct gaaattacga tgaatttaac
    32521 cacctccgac agttatgatg tggaagtcat caatggtttt cacatcccga tttcgatgca
    32581 acccatttat tatcaaggag tcactactat tcccgctatt cctgataatt acaattgcgg
    32641 agaacccggc aaagacactg cagccaatgg atttggagct tgcgattgga gtacagccac
    32701 agtgccagtc attgatcaag tgccagggaa tggattttat tgggtaactg gtggggggca
    32761 aggttgctcc attacctctg caaatccggg ttgccctgcg atgacattat gtggattaga
    32821 cagcaatttt aatcaggttt gtggcaactt tttaggatat tggtccgccg atcaagtctg
    32881 cggttcaagt aatgtcccag ctgccgtaca gagttatttt aaatgcaatc aacccctccc
    32941 tacttctaca acgccttttt atccttccgg cgctgtctta tccaacttga tgctatgctc
    33001 tgtttccaca ggatttaccg gtccaaggta taacacctgt tataatgctt atccttcttc
    33061 ttcccccaca gacattgccc aatgttgtgg gtgtgtagat tggtggaata ccgctcaaac
    33121 caacaatgta gcgattggag caaatcctaa taccgaaagt tgtactcagc ctggcgcctc
    33181 gcaaccacaa accaatgccc agtggaattc atttgtacaa ccaatgatac aatggatgaa
    33241 acgagcttgt ccttctgctt atatttatcc ttttgacgac aaaaccagtg gatttacatg
    33301 tactaacaac ctgtcagggc aacctaactc cacaagctat ataatcaggt tctgtccagg
    33361 aagcatcaca gggcttccag caggagtaaa tgaagggcgt ggataaaata tatagctggt
    33421 attaacgctt cgtactttat agttgcaaga agcgagtcgt taataccggt aaacatgaac
    33481 aacaattact ttgatataat ttcctgcact tcaaagatgc gtctgtcagc aaaaaattta
    33541 gcttgaaaca aatgaatacc ttcctcacgc atcttaggtg ccaactcaga caagtacctt
    33601 gacaaattat ctctattgtc cagctggtat tgaacagtca atttttcctt acctgatttt
    33661 gtttcttttt cctgctttag aatgcaggcc tgcataaatc ctggaaattg gattatttcc
    33721 gcaacatgtt ttttgagcca tgcttgaaat tgtacaaata tctccggatc aatcgttatg
    33781 tcgttgctga atataaactg atcccctttg ccgactgaaa attgatcccc ctactatagt
    33841 tttcttcagg agaatgaatt ctggaggagg gaatgattca attagaggag tcaaaaatga
    33901 ttcatatttt gtacaagcag ggatacagta aaaaagcgat tgctagaaaa ttgggtatgt
    33961 cgataaatac tgttcgaaaa tatcttaaaa gtggttcaga gccttattac aaaaggcgtg
    34021 ataaaaagcc attaaagctt gacccctata aagcatatat tcaaaaaaga ttaaaagatg
    34081 caacacctca ttgggtacca gctactgtga tttatcgaga gattaaattg tttggttatg
    34141 atggatgcag ctctcaatta agagcatata tgcatacttt aaggtcaaaa aaagaagagg
    34201 ttattgttcg gtttgaaaca gagcctggac agcagatgca ggtggattgg gctcaattcc
    34261 gaagaggaaa ggatagatta agtgctttta ttgcaaccat gggatattcc agagcatctt
    34321 atgtagagtt tgtcagcgat gaaaccttgg aaacgcttat tgcatgccat aaaaatgcat
    34381 tcgaatattt tggtggagtt ccttatacga tcctatatga caatatgaaa acagtcatta
    34441 ttgaacgaaa tggatatggc ccaagccaac atcgatttca agctggtttt tgggattttg
    34501 caaaacatac tggatttcgt cctaaagtgt gtcaacctta tagggcacaa acaaaaggca
    34561 aagttgaacg ctttattcat tatttaaaat acagttttta ttttccttta gttgggcaat
    34621 tgaaggcact tggcttaagt ttagataaag agacagccaa tatgcatgtt ttaaaatggc
    34681 taaatgaaat tgctaatcaa cgagtacatg ctacaacagg agcaattcca ttcgaacgac
    34741 ttcttgatga acaagcaaaa ctacaaccat tgtcatctac ctattctggt aaattatttt
    34801 ctcatttgga aaataaggag gttcactatt ttgcctttca attattagac aacactgcaa
    34861 tgcaacatga attgtcaatt tatcagaaac tactcgagcg cacagaggag gctgcatgaa
    34921 tattcaacat gagcgtattt tatcactatg tgaacgcttg aatttatcag cgatagcgac
    34981 aaattactct tatctagctc aagaggcagc aattcataat caaggatata gtgatttttt
    35041 agaaagtgta ttgactgtgg agttacagga aaaacaatat cgaagtcgaa ccctgctcac
    35101 taaaatggct ggttttccaa tgattaaaac aatagatgat tttgatttta aatttgcttc
    35161 tggtgtacca aaaaataaaa tccgagaact gacttctttg gctttcattg aacgacagga
    35221 aaacatttta ttacttggt
//
LOCUS       DATNSN010000014        35103 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.014, whole genome shotgun sequence.
ACCESSION   DATNSN010000014 DATNSN010000000
VERSION     DATNSN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35103
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.014"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2384
                     /locus_tag="VAH76_000569"
     CDS             <1..2384
                     /locus_tag="VAH76_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEO1385218.1"
                     /translation="HLKGQLLDQFSIGEMEYQHQGTKIKIFNLAVNWRFSSLLHKKLP
                     IDSMSAEMIEINQKTPILILKNIKLSGYLNQQSIKLNTLQFNYWNQLITSQLELSNSF
                     PHVFSGKIRLNPHSTNKNTFSGGIDIGGNRNQIQWGGEFEGPGKITINGSLQQLKQIK
                     QVIKWRDLHWQDNKNNNLKSSQGRIEVSGTIPNLAIDLKSKLSIDQLKNWQMNASIKG
                     KLPWQWNVDATFSQPLDFSSKLDGLYTKFALKMETQNDHHADFLITIAPGHYQMTENN
                     LIPLIQFTGGNIKGSLSQKQFSGQGVLFLDAYKKVNFNFKFPEFNVYQDIAQQTIDAK
                     LSILFNSLDFLQNASPYLINPKGQLLASIHAKGTLNKPIIESKLALNKGSFNIPALGL
                     NITDMGLAVFSKKNGWEATGSIHSSEKKLTLKGQGIFNKELGGTVSIEGSNFPIVNNK
                     EYQINISPQLHVRFTPSLCQITGTIQIPNAHIKPQTFTNSLVATDDIVFTSKNTQPIT
                     PLFNTNMSVRVEMGEEVELTFKGLHAYLGGIVTINQLSQGPITANGELTVKKGEYKAY
                     GQDLSIEQGQLIFTGGRIDNPEINLRASKNISNSFGSVSGSSELFNFNNYNNLNVGNN
                     FNVGVEVAGRLQSPKIQLFSNPPTLSQADILSFLVIGRPANQADKAKGQLLLTAISSM
                     NLGTGTNGTQLLEQLKQKLGFDFNVQTTTNFNQQTNKISESTGVVVGKSLSERIYLSY
                     NVGLSQTDPNVLTLKYILNKFLSIQVSSSDSGNGVDVLYTKSTSSPEKAKPDK"
     gene            2418..3413
                     /gene="hemB"
                     /locus_tag="VAH76_000570"
     CDS             2418..3413
                     /gene="hemB"
                     /locus_tag="VAH76_000570"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEO1385219.1"
                     /translation="MNEYNLPLRLSRLRKNAMSRALVRETRLHTQQLIAPLFISEYLT
                     EAKEISAMPGQFQLGLNCIPEEIEALSKLGIPAVLLFGIPKQKDACGSESMNNNGIIQ
                     QAIKKIKSVNSDMLVITDLCFCEYTDHGHCGVLDGSQINIASTLELLGKQAVSHAEAG
                     ADWVAPSGMTDGMVLAIRQALDTASFHDIPILSYSAKYCSCLYGPFREAAQGAPQFGD
                     RKAYQMDPANSEEAVRETRLDLEEGADMIMVKPAGFYLDIIYKLKQHFADVPLCAYQV
                     SGEYSMIKNAAKNNLINEEQAMTESLIAIKRAGADFIISYFAKGMAELLNSQNFI"
     gene            3550..3942
                     /locus_tag="VAH76_000571"
     CDS             3550..3942
                     /locus_tag="VAH76_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385220.1"
                     /translation="MNLMLKGLSALAISLGAATTFAAPAYLTTHNRTGEESNAYIAGS
                     IPSLYPTAAYSTNQVYWNLVRLACYGHTTNGQCPALIKMATNTANPIDIGYVTMDLNT
                     GDITPKTLSAKGYSLRVIGPGEAEITKN"
     gene            complement(4240..5682)
                     /locus_tag="VAH76_000572"
     CDS             complement(4240..5682)
                     /locus_tag="VAH76_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEO1385221.1"
                     /translation="MKIRHKPLRTLVSTAVLGVLATGTLNAGGFSLYTEGAGYTTGNF
                     AAGVAAEAADASTGWYNPAGLVLIRDQQAVFAGVGVFPTAKLSGVSTYSTINPPPFPS
                     FVYSEVFNNINGAEDAFVPSFHYALPLGENATFGLSVVSPFGLATDWGPDSPVRYQAT
                     FTELITTNISPEIGGRLTEHVALGAGLDLQYARVKYNRILGVPNILNVLPPFNPMLAD
                     SLTYNKGHSFGVGFHVGVLGMFNDNHTRLGANYQSSMRHEFYGYSRLTGPLASPGNIL
                     APFLFPAPGIFQSDDLFSNSIDFPDVVTISAYHDVNDKLALLGSLVYTAWSSYKTVQL
                     SNAAAGRPADNGNVSQAKAISISPQNYNDTLRVAIGANYHVNDKLMLRVGGGYDPTPT
                     NNIDRDVRLPDTDRWALSAGAHYQATNNIGLDIGYTHLFTSSDPVINRTDAFTATTRY
                     NVTASGTAGANLVGVQATWTIDKEPPAPTK"
     gene            6022..8583
                     /locus_tag="VAH76_000573"
     CDS             6022..8583
                     /locus_tag="VAH76_000573"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HEO1385222.1"
                     /translation="MQQVVIKFGGTSVSTRATWNNIAAITQKHLNTGVQPVIVCSALT
                     QISNKLEKAIEAALLDEHHSLFNDIQSSHLNLAEQLEVNHQLIANDLHQLQQWLTGIS
                     LLKQAPAKTHAQILSLGELMMTRLGHAFLEKQGIKVKWYDARELLTSTPTLGGETMNY
                     LSARCESEYDSALVEKFLSSGAQAIITQGFFAANPHGETVLLGRGGSDTSAALLAGKL
                     QAASCEIWTDVPGIYTANPHQLPHARLLKQLNYDEAQEIASMGAKVLHPNCIPPVRKA
                     NIPMVVKYTHLPEHSGTLITKDIDESAPLIKSIQVKHSILLISIDTLNMWQQVGFLAD
                     VFAAFKKHGFSVDLLSSSEFNVTLSLDVNAKIHDRPAINALLDDLNQFGRAKLIEPCS
                     AVSLVGHHIRTVLPHLGPALEVFEAKQVYLMSLASNDLNLTFVVDESHADKLCQRLHH
                     LLIESNPQIFYYSKSWHEEFGKPNVRPTPWWEIERDRLLTTSAIHSPCYVYHSPTQAT
                     RARQLSALESIDSLFYAIKANPFPTILKTLEKEGIGFECVSIQELELVLKLFPNIKKE
                     RILFTPNFAPKSEYEFALQTGCYVTIDSLYPLENWPDLFKDREVIVRIDPGTGAGHHK
                     HVSTGGNESKFGITQNDISKILSLTKVNHIKVIGLHAHSGSGILSTDLWQQTAVMLAS
                     LTDQFPEVRSINLGGGLGIVEKPGQHPIDFAALDAQLMAVKSQYPGLAIWLEPGRFFV
                     AESGVILAKVTQCKEKGKVKFIGIETGMNSLIRPSLYGAYHEIVNLTRLHEEKAGFAH
                     IVGPICESGDTLGYDRLLPVTREGDVILIANTGAYGHCMSSHYNLRPPAQEIVLE"
     gene            8588..11818
                     /locus_tag="VAH76_000574"
     CDS             8588..11818
                     /locus_tag="VAH76_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEO1385223.1"
                     /translation="MRLIDSEQRSQATDPAESFIVQAPAGSGKTEILTQRFLRLLSTV
                     QSPEQIIALTFTRKAASEMRERIVQALHMAASNQPAVSAHQQSTLNFAKQALQRNNQY
                     QWDLLEQPNRLKIITIDSLCQSINRAIPLLEKQVAYPDISETPDSHYISAARCCIQYA
                     IATPEYQEAIKTLLLHVDNKQERLIQLFQALLSQRDQWLPTLFLAREQEKSTFEQALR
                     LIEQHELSRLRNSLPSDLAQELVLLSRELAVIENKPDSPRFILREWYEFQKCDQQIAG
                     ALSKLLLTSDNQLRKSFDHHVGLVKKNCAPNEFTRIKTASSDLFAKLQDCPDFLDALL
                     KVNQLPTPEYDKEQWDVLQALFLLLPLLVGHLQVSFGEHNEVDFTAISQQALAALGDL
                     DNPTDLALYLDHSIHHLLIDEFQDTSITQFELLTKLVQGWLPGEGKTLFLVGDPMQSI
                     YRFRQAEVGLFFQAKEHGIGPVRLKSLELRCNFRSTETIVNWVNDHFSKIFSQQVDIE
                     SGAVSFHPSVHVIEKNESSTIQAVQFKNREQEARHLIEIINSELNRNPEQTIAVLVRT
                     RTQLPEIISLLRQNNIPYQGTDIDLLANLVHLRDVWSLAHALLFPGNRLSWLSILHSP
                     YVGLGLSDIHCIAQYNSRKSIYHNLLHLDKIQDISEEGRIRANFFIQIMNQALLCRSH
                     SRLSDWIADTLKNLHVEKILDKAQLNDLEQLWMLLDQYEKEGRIPDMNEFLNEFNKLY
                     AQQTTPSRLQIMTIHKSKGLEFDTVILPGLGSQANRGESPMLRWLKLPTQDHGNLLLV
                     SPIKSANQDNCPLYDYLDQLDEEKSAYEAQRLLYVAVTRAKSRLYLLDHSAKSYKSSF
                     RNLLKYQEFIADPDEAQHEEQEFPLPKLVKLPIEYYQKQIPSLVQSQESSCPEISSGI
                     PRLIGIVSHQLLQWICDNHPQTINHVPWNLVHNELKALGLNQEAQQTALSMIKIQISQ
                     MYENKIGLWILSRHENEQNEYELLVEYENKLVTRIIDRTFTENGTQWIIDFKTGKEDI
                     NTLQKHRQQLNEYGRYLSDCTKFPIRCGLYYLANGHWIDWQYKMSA"
     gene            12025..13098
                     /gene="apbC"
                     /locus_tag="VAH76_000575"
     CDS             12025..13098
                     /gene="apbC"
                     /locus_tag="VAH76_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEO1385224.1"
                     /translation="MTIEDTVIKLIGSLKDPLLDLTGKEMNLQYKITTSNQAMHVVLT
                     AGYPTSLLETSYKPIAQKIVQDEFPNYQVTISIQQFIKAHKTQLTGKALRGVKNTIAV
                     ASGKGGVGKSTVTVNLAAALAKLGARVGILDADIYGPSIPLMLGETKPVQVKDNCYIP
                     VEAHGMQAMSIGYLTDTNQALIWRGPMLAKSLIQMLDITLWNELDYLFIDLPPGTGDI
                     QLTLVQKIPLTSAIVVTTPQNVATLDAQKAITMFSRTGIDVLGVIENMSTHICSHCGH
                     QEAIFGRGGAAALCDAYQCTLLGQLPLDSHVRRHCDEGVPTATHSSNQLTDTFIKTAM
                     RTAIELSKKPINYADRFPKIVVE"
     gene            13214..13768
                     /locus_tag="VAH76_000576"
     CDS             13214..13768
                     /locus_tag="VAH76_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385225.1"
                     /translation="MIRLLIITISVLCSGLTIAKSSYQIDLILFAQPQNGVKSIKSDS
                     PVPLIPVSPNAIQLHSDNNKKHMPYTLLPRSQSKLSDQHYLLSRKSQFQILGHYSWRQ
                     PAKSDSLVALPKINHNGWQIQGTLKVRQSNYYLFDADLQCLSPGNQETSFTVSQKQRL
                     KGSTIYYLDHPQLGMLVKIHKIAS"
     gene            complement(13816..14706)
                     /locus_tag="VAH76_000577"
     CDS             complement(13816..14706)
                     /locus_tag="VAH76_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1385226.1"
                     /translation="MNLRDLHYFVILADVKHFGEAAKRCFVSQPTLSMQIKKLEEELG
                     VVLFERTNKQVLLTDQGSKLLDRTRKILILIDEMKELARQSEDPFTGELRLGVIPTVS
                     PYMLPLVMPELKNEYPRLKVWLIEDQTHRLITKLEQGELDVAIMALPIDKRFSCQTLY
                     EEKFYFACANTHPLAQAKSVNINDLKNQPIMLLEEGHCLREQAMAVCQSAKADDIADF
                     TATSLETLRLMVQAGMGVTLLPALSTLTASTNHLKCIPFSEPAPSRILGLFWRAGTPR
                     QVCFNAIAELITKNVQSKLV"
     gene            14966..16138
                     /locus_tag="VAH76_000578"
     CDS             14966..16138
                     /locus_tag="VAH76_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385227.1"
                     /translation="MVGVFFLLFQFFLQLSSGIIVGAIMHEEHISALSAGLLSSAFYY
                     VYTSFQIPVGILFDRYNTRYLLSVNATLCAIGCFVFASGHNLPTLFLGRLIIGAGSAF
                     AFIGLSHLLRQHFPLKQYAFMIGLSETLGFTVTVLGMIGMGSVISHFGWRYFINIAGI
                     IGFLIASLCWLCIPNSKPVVSFPHQYKQQIIPILKNKLAWFNGLFVCLEFSVITVFAA
                     MWAVPFLQLKLDCTIKTASILTSMVLLGAGLSCPLLGQISVHLNRRKPLIHASCLSTA
                     LLLILVLYLPTQNKLLIGILMFSMGLTCGAYMLAYSISNELAPPEFLSTCTGFTNTLA
                     MLSAPLLQPLVGFFLDVLSNHKSQYSLADYQLALLIVPFAMLLASLFTCLLPEKSN"
     gene            complement(16140..17090)
                     /locus_tag="VAH76_000579"
     CDS             complement(16140..17090)
                     /locus_tag="VAH76_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385228.1"
                     /translation="MMPKRIWLLLLSMFLFLSGSVFAVEEPEEDKVAQTNEPTVGSWV
                     FTGMVTNESGDRYGYFFQMQRQGTDFYAKTALIDGQTNQLILFYEANEKIDSSTQLNW
                     QVGRSFLRYNPINDSWIFGVKVENAKGFNFKVDMLKQDNKDVETQVLRPGVELQAVQT
                     SRLNGHVQTGVDNKEQFVTGNKAWFSKLWFSKDQNSPHDISTIFCRLSNDNGFYSANL
                     KEPDATGAAVAGWRDPSGNNVKMSQFVSIKTLTDNQCLLSVGLPRLKLKLVNTIKQED
                     DFPLTVAGFSEENPKGFCFISKQTFKQIKESAVQAVTNLG"
     gene            complement(17092..18063)
                     /locus_tag="VAH76_000580"
     CDS             complement(17092..18063)
                     /locus_tag="VAH76_000580"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEO1385229.1"
                     /translation="MSFFVNRIWYGNHFLQWILVPFSWLYRIVIRTRRWYLQRFCQQL
                     YPIPIIVVGNVTVGGVGKTPLVIEIAKKIQQKGLKVGIVSRGYKAAIKHFPYEVKLND
                     SAELVGDEPLMMARKINCPVVIAPKRNEAVRYLLDKHSVEIIISDDGLQHYKMGRSIE
                     IVVIDGMRKLGNGFCLPAGPLREPDSRLKQVDFVIVNQGAAEGTYSMELIPKNIVRLS
                     TQEEVSNDLFTSEVAAVAGIGNPQRFYSTLSQLGIKFNPYSYPDHHQFKPHDLNDIDL
                     PVIMTEKDAVKCYSFSSDKLYYLPVEAKLNDSFWEAFWSHQQLQGYY"
     gene            complement(18063..19829)
                     /gene="msbA"
                     /locus_tag="VAH76_000581"
     CDS             complement(18063..19829)
                     /gene="msbA"
                     /locus_tag="VAH76_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEO1385230.1"
                     /translation="MKNNLPIKSRLLYKRLLSYVKPFWPVLLLGVLANILYSGIDAGF
                     TYMTKLFLDKSFITIDLNFVKQIPLIVLIGITLRGLVSSLGSYCMTWVARSVVKVLRQ
                     TVFSHIIHLPADYYDEATSGQLLSKILYDVEQVAQVSADALTDFIQNICLVIGLLTVM
                     MVICWQLSLMFLLTIPFVGIIVNYTNKRVRRISHKVQKTMGEVTEIASEAIEGYRVVR
                     IFGGERYEITKFNKATEYSRKNDMKVAISKAINVSGVQLVIAIGIATIIMAAIHLSTV
                     ITISAGSFLAIIAAMLQLIKPMKTLTTLNATIQRGLAGAESVFNLLDLPLERNNGLIL
                     KEKIRGEIEFKHVYHAYRQGQNILHDVNFVIEAGTSVALVGHSGSGKTTIASLLPRFY
                     ELSQGMITLDGMPIQQLSLESLRKQMSLVSQNVTLFNDTLANNIAYGRFDASREQIIT
                     AAKLAYADEFIKQLPDGYDTRVGENGVLLSGGQRQRIAIARAILKDAPILILDEATSA
                     LDSESEHYIQAALEQVMKGRTTLIIAHRLSTIKHAHKIIVLQHGRIVEQGSHQELLDM
                     DGHYAQLYKVQQFGRINEEVVA"
     gene            complement(19916..20698)
                     /locus_tag="VAH76_000582"
     CDS             complement(19916..20698)
                     /locus_tag="VAH76_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385231.1"
                     /translation="MYITKELAVRMEGCIKQSHIEVTKRYSQGKILEINGGAACFSGF
                     ESFLSQVVGWGFKTEPNQYKAEIEAIENFYKSLGHNRVDIELCPFVGNHLINFLSQRG
                     YYITELNNVSVLDLKSYHLVAHSEDDGFIIREVKQSELEEWAKRVAIGFGFLEAQEQF
                     YQYASAKGVLAFAAYDHGNLVAGATIAIHGDACDLGVTSTLPLYRGKGLQKKLLFARL
                     NLAKQYGLEFATVTTQPGTVSDLNVQKAGFRCAYTRIKLTME"
     gene            complement(20719..21720)
                     /locus_tag="VAH76_000583"
     CDS             complement(20719..21720)
                     /locus_tag="VAH76_000583"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEO1385232.1"
                     /translation="MYSLLRPLLFRLDAEKAHSLTLSLLHYLPGFYFRKIAGQPVHAM
                     GLVFPHQVGLAAGLDKNGEHLDALAKLGFSFIELGTVTPKGQAGNPKPRLFRIAEANA
                     IINRMGFNNSGVDVLVENVKRANYKGILGINIGKNKETNLNQAAEDYLYCFRKVYDHA
                     SYVTINISSPNTPDLRQLQQGDYFAELLTQLQKEQIKLADQYGRHVPLVVKVSPDETD
                     ETLKQMTDIILQYGIEGIIATNTTCSREMVKNLPCSEEQGGLSGRPLMELSTRCLRLL
                     KQYVGNDVTLIGVGGIDSLESAKDKINAGASLLQVYSGLVYKGPELIHDIVSGLNAI"
     gene            complement(22006..23004)
                     /locus_tag="VAH76_000584"
     CDS             complement(22006..23004)
                     /locus_tag="VAH76_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385233.1"
                     /translation="MAVSNKKNSEFTPSLRSHMIAALLSMGAAAIILWLASGMTLAAV
                     IVDSFSLGFYIGFMLLMSAAPAVLASPVILPGLLLVVSGLFLGAVAATFAHRLKNNPS
                     LPQNNNGNGLINELTKDKSELLISHDDLQCQSNSLEGENTLSQQNEDNVTGEKFGFMK
                     FWGSLGLTSFASSGATALLGWLATDFVVSLSIIPVFLLGMTLAIVLVPLSIGLSGIFA
                     LPAVTALMFALGGFATGVFAATLAAYFIADNQAQDHPVEPIIRSSMERQCKDQGSHQK
                     ILNGLKKDKLSANEEVINSDLNEQKSRGPKTSKSDTGVKEEHINTAEEYSFCGLRF"
     gene            complement(23053..23739)
                     /locus_tag="VAH76_000585"
     CDS             complement(23053..23739)
                     /locus_tag="VAH76_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385234.1"
                     /translation="MRKLVLWGHGIEDYREMFDLPQGVENSRLLEYGCGPSAVNYQQT
                     QVKKNGVVSCDPLFVLDKDTLLAKAKMIFANMAEEVRQHQDQFDFSRNGSLDKLLQER
                     QEGMKQFFADYEQGKADGRYLGLTDYHLPFADFTFDFALSSHYFFADLDDQTVDFHLI
                     AIRELARVAKEVRIFPLIDREGNTSQFLGPVLLGLQQENYGVEVREAAFHLHKSGNAM
                     LRVWAQQCVV"
     gene            24074..25234
                     /locus_tag="VAH76_000586"
     CDS             24074..25234
                     /locus_tag="VAH76_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEO1385235.1"
                     /translation="MYTGLDFQLGETYDMLRDSVNQFAQTEIAPLAAQIDEKNTFPNH
                     LWRKLGEMGLLGITVSEEYGGANMGYLAHAIAMEEISRASASVGLSYGAHSNLCVNQI
                     FLNGNNEQKQKYLPKLISGEYIGALAMSESNSGSDVVSMQLQARSAGDKYILDGTKMW
                     ITNGPDADVLVVYAKTDKQAGSKGITAFIIEKGMPGFKTAQKLDKLGMRGSNTCELVF
                     DQCEVPSENVLGTVNQGVKVLMSGLDYERTILAAGPVGIMQACMDVVLPYVHERKQFN
                     QAIGEFQFIQGKLADMYTDLNACRAYLYAVAKACDNGRVSRKDAAGVILYTAEKATQM
                     ALQSIQILGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFKETA"
     gene            25246..26430
                     /locus_tag="VAH76_000587"
     CDS             25246..26430
                     /locus_tag="VAH76_000587"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEO1385236.1"
                     /translation="MEHNDVVIVAAKRTPMGAMLGNLSALSSPELGAVAHMAALSQSG
                     IAPAEIDEVISGCVLQAGIGQAPARQAAIKAGIPNSTGATTINKMCGSGMKAVMLAHD
                     LIKAGTANVILASGMESMSNAPYLLSKARAGYRLGHGELKDHMFLDGLEDAYDKGQLM
                     GCFAETTAKHFNFSREQQDEYALRSMTKALKAIENGAFREEIAPVTLTTRKGDITVDV
                     DEGPETAKLAKIPQLKPAFQADGTVTAANSSSISDGAASLILMSASNAEKRGIKPLAK
                     IIAHASHSQAPQWFTTAPVDAIRKVMNKASWKQDDVDLFEINEAFAVVAMAAITQLEL
                     NPEQVNIHGGACALGHPIGASGARILVTLMHALKHQGKKRGIASLCIGGGEATAMAIE
                     LI"
     gene            26568..26882
                     /locus_tag="VAH76_000588"
     CDS             26568..26882
                     /locus_tag="VAH76_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385237.1"
                     /translation="MLRHSLIYLLLSILIVVFAKYAHLIVVYIDMFFTYVNLKLTPIF
                     SQTGWGLIIRKILVLMLLPIIIAGIPALIYRALKGKDMPHFIAIVWIIWTIIVLSDIL
                     IR"
     gene            26892..28499
                     /locus_tag="VAH76_000589"
     CDS             26892..28499
                     /locus_tag="VAH76_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HEO1385238.1"
                     /translation="MAKLTTQINTSSQEFKNNQANMQALVTDLREKIHQISLGGDEKA
                     RTKHQQQGKLLPRERLHQLLDPGSPFLELSQLAAYQVYEDTIPAAGIITGIGRVAGNE
                     CVIVVNDATVKGGTYYPLTVKKHLRAQEIALINHLPCIYLVDSGGAFLPLQDQVFADK
                     EHFGRVFYNQAQMSALNIPQIAVVMGSCTAGGAYVPAMADESIMVKNQATIFLGGPPL
                     VKAATGEVISAEELGGAEVHCRHSGVSDHYAENDAHALHLARVAISNLNRKKPDSIHR
                     VDTVPPLYDSEDLTGIIPTDPRKPFDIREIIARVVDGSEFDEFKALFGTTLVCGFARL
                     YGYPIGIIANNGILFSESAQKGSHFIELCCQRKIPLVFLQNITGFMVGSKYEASGIAK
                     HGAKMVTAVANANVPKFTIIVGGSFGAGNYAMCGRAYAPRFLWAWPNARISVMGGEQA
                     ANVLAQITREKYAKQGKEWSLEEEEQFKTQMRSQYETQGNPYYASARLWDDGVIAPQD
                     TRKILGLGLSAALNAPIEDTRFGVFRM"
     gene            28519..29313
                     /locus_tag="VAH76_000590"
     CDS             28519..29313
                     /locus_tag="VAH76_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEO1385239.1"
                     /translation="MSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAIND
                     TNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSLSQSPKPTIA
                     MVQGSAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKML
                     FMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVAN
                     KKIDEELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV"
     gene            29306..31270
                     /locus_tag="VAH76_000591"
     CDS             29306..31270
                     /locus_tag="VAH76_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEO1385240.1"
                     /translation="MFNKILIANRGEIACRIIKTAHSMGIQAIAVYSAADRNSLHVRL
                     ADSAYYIGEAPAKESYLNIDHIIQAAKESGAQAIHPGYGFLSENPDFAKACEQAGIVF
                     IGPSIKAMEAMASKQLAKQLLEKTKVPLTPGYHGVEQSEEKLLSEAKKIGFPVLIKAA
                     NGGGGKGMRAVHDEKEFHDALAGAKRESMASFADDTMIIERLVLNPRHVEVQIMADNH
                     GNVVHLFERDCSIQRRHQKIIEEAPAPNLSPMLRQRLAEAACEVARSINYRGAGTVEF
                     LVDGEDKFYFMEMNTRLQVEHPVTEMITGLDLVAWQIRIAANDTLPLLQNQIQAQGHA
                     IECRIYAEDPYQGFIPSIGQLQFLKEPSGDGIRIDTGVTLSSEITRYYDPMIAKLIAW
                     GHNREEALQRLERSLSHYDIGGVKTNIPFLRAICRHVKFKEAKLSTDFLEKENINLPK
                     PDNELGILLAISCDYLGMVNRTTDPLLQDAFGWQMHLSSHWVWRYQLNSTIIEAQITP
                     IDSKKFKAKIENKEMVIHARYDLDQLIIEIDQKSVKARVENKEHHLIFYTDKGQLSIE
                     RFYWSKLDAQTSAHKGQLTAPMPATVVAILKNIGEQVKAGESLIVLEAMKMEHTIHAP
                     IDGILSDIFYSVGSQVSEGAELLALSESDT"
     gene            31281..32189
                     /locus_tag="VAH76_000592"
     CDS             31281..32189
                     /locus_tag="VAH76_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEO1385241.1"
                     /translation="MDYPQHVTIIEVGPRDGLQNEPSFLPSDKKIELINLLSQTGLKE
                     IEVTSFVSAKAIPQLADNEEVFQSINKTPSINYSALVPNERGMLKALEMGVQNIAVFT
                     AASELFNQRNINCSIKESIERFKPVLALAKTNQIRVRGYISCVLGCPYEGYIQPSQVV
                     SVTKMLLDLGVHEISLGDTIGVGTPRQTQLLLDAILPILPITQLAMHFHDTYGQAVAN
                     IYASLEYGVNRFDSSVAGLGGCPYARGASGNVATEDVLYLMHGLGIDTGVDIFKIVAA
                     GDMICKALGRKNQSKVANAMLANPCN"
     gene            32346..34286
                     /locus_tag="VAH76_000593"
     CDS             32346..34286
                     /locus_tag="VAH76_000593"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEO1385242.1"
                     /translation="MNDKVWTPKNPEQTRMWQFMDFAAKRHCQVFENYQDLHTWSVKH
                     PESFWETLCLFFKLNFDTPPHQILNYYSEMIEARWFSGARFNFAEKLLTRRDNHPALI
                     SLDENDSKSVISYAELYEKVAQCAAGLKAVGVATGDRVAAVMPNTSYTIIAMLAATSL
                     GAIWSSCSPDFGTQAALDRIGQIEPKVLFICDGHQYQGKKHSSSEKVVQLTAAITSLR
                     QVVICPNINEEINLEQLPNASYWNDFIKPEKECDFISLPFDHPVYILFSSGTTGKPKC
                     IVHGAGGTLIQHLKELGLHSDIKSTDNLCFYTTCGWMMWNWTVSALALGATITLYEGS
                     PTYPTDNRMFRLIDEEKVTVFGTSAKFISAIEKAGVKPKSEFQLSELRCILSTGSPLL
                     PKNYDFVYEHIKKDLQLSSISGGTDIISCFALGNPILPVYRGELQCIGLGMEVAVYDE
                     QGHSIKQKRGELVCTKPFPSMPVCFWNDKDRQAYKHAYFERFPGVWAHGDFAEITAHN
                     GLIIHGRSDAILNPGGVRIGTAEIYRQVEKVDDVVDSIVIGQDWQDDVRVVLFVKLRE
                     GKKLDDELINLIKTTIRKNASPRHVPAKILQVPDIPKTLSGKVVELAVRQVVHGQSVS
                     NLQSLANPQALEYFKDRDELKG"
     gene            complement(34456..34872)
                     /locus_tag="VAH76_000594"
     CDS             complement(34456..34872)
                     /locus_tag="VAH76_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HEO1385243.1"
                     /translation="MKKMFLAVFTLCSFTAQAAVELKTFQNIVDAIAEGKRITFVINL
                     KKCTSEMPLNSAIVSVTPNAVMVVGDSRVTASDRHFTLDDPVARGTPMFDYSKFNLDS
                     EGDASIKTTVLNASSYERLGSYQMNCKLGDGFKVFG"
BASE COUNT        11226 a         7088 c         6530 g        10259 t
ORIGIN      
        1 accatcttaa aggacaatta cttgatcaat tttctattgg tgaaatggaa tatcaacatc
       61 aaggcactaa aataaaaatt tttaacctgg cggttaactg gcgtttcagc tcattgcttc
      121 acaaaaaatt gcctattgat agcatgagtg ctgaaatgat tgaaataaat caaaagacgc
      181 ccatactgat actaaaaaat atcaaattaa gcggttattt aaatcaacaa tcaattaaac
      241 tgaatacact gcaatttaat tattggaatc aattgattac cagccaatta gaacttagca
      301 actcatttcc tcatgtattt tcaggaaaaa ttcgtttaaa cccacattct acaaataaga
      361 atacattctc aggagggata gacattggcg gtaatcggaa tcaaattcaa tggggaggag
      421 aatttgaagg tcctggaaaa ataacaatta acggcagtct tcaacagtta aaacaaataa
      481 aacaagtcat aaaatggcgc gacttacatt ggcaagacaa taaaaataac aatttaaaaa
      541 gctcgcaagg aagaattgaa gtatcgggaa caattccaaa tttagctatc gacttaaaat
      601 ccaaacttag catagatcaa ttaaaaaatt ggcaaatgaa cgcttcgatt aagggcaagc
      661 tgccttggca atggaatgtc gatgctacgt tctctcaacc attagatttc tcatcaaaac
      721 ttgatggttt atatacaaaa tttgctttga aaatggaaac tcaaaatgac catcatgctg
      781 actttctgat cacgattgct ccaggccatt atcaaatgac agaaaacaat ctgataccgt
      841 taattcagtt tactggcggc aatattaaag gctctctttc ccaaaaacaa ttcagtggac
      901 aaggagttct ctttctggat gcatataaaa aagtgaattt taattttaaa ttcccggagt
      961 ttaatgtcta ccaagatata gcacaacaaa caattgatgc gaaactatcc attcttttta
     1021 actctctgga ttttttgcaa aatgccagtc cttatttaat aaatcccaag ggacaacttc
     1081 tcgcctccat tcatgcaaag ggaactttaa ataaaccaat aattgaaagt aaactcgctt
     1141 taaacaaagg ttcatttaat atccctgctt taggtctcaa tataactgat atgggattag
     1201 ctgtttttag caaaaaaaat ggatgggaag cgactggctc tattcattca tctgaaaaaa
     1261 aactgacctt aaaaggacaa gggattttca ataaagagct tggtgggact gtttcaatag
     1321 aaggttccaa ttttcccatt gtaaataata aggaatacca gattaatata tctccacagt
     1381 tgcatgtgcg cttcactcca tcgctatgtc aaatcactgg aaccatccaa attcctaatg
     1441 cccatataaa accacaaaca tttaccaata gtcttgtggc tacagatgat attgtattta
     1501 cgagtaagaa tactcaaccg attaccccac tctttaatac caacatgtcg gtacgagttg
     1561 agatgggaga agaagttgag ctgaccttta agggattgca tgcttatttg gggggtatag
     1621 ttacaatcaa tcaattatcg caaggcccca taacggcaaa tggtgagtta actgtcaaaa
     1681 aaggagaata taaagcctat ggccaggatt tatctattga acaaggacaa ttaattttta
     1741 caggcggtag aattgataac ccagaaatta atttaagagc atcgaagaat atatccaatt
     1801 catttgggag tgtatcagga tcctctgagc tgtttaattt taataattat aataacctca
     1861 atgttggcaa caattttaat gttggggtcg aagtagctgg acgattacaa tcgcctaaaa
     1921 ttcaattatt ctctaaccct ccaacacttt ctcaagcaga tatcctatct tttcttgtaa
     1981 taggccgccc agccaatcaa gcagataagg caaaaggcca attgcttcta acagctattt
     2041 cctcgatgaa tttaggtaca gggactaatg gaacccaatt gctggagcaa ttaaagcaaa
     2101 aattaggatt tgattttaac gtacaaacaa caactaattt taatcaacaa accaataaaa
     2161 tcagtgaatc aaccggtgtt gtggttggaa aatctctttc agagcgaatt tatttaagtt
     2221 ataacgtcgg tttgtctcaa actgacccta acgtattaac attaaaatat atactaaata
     2281 aatttttaag cattcaggta agcagcagtg attcaggtaa tggcgtcgat gtcctatata
     2341 ctaaaagcac atcatcacca gaaaaagcaa aacctgacaa ataaaatctt aatccatact
     2401 gtcgaaggat gtaataaatg aatgaatata atttgccttt aagattatct cgtttaagaa
     2461 aaaatgccat gtcaagagca ttagtaagag agacgaggct gcatacacaa caactaattg
     2521 ctccattgtt tattagcgaa tatttaactg aagcaaaaga aatcagcgct atgccaggcc
     2581 aatttcaact aggcctcaat tgtatacctg aggaaattga agctttatca aaattaggca
     2641 tccctgcggt cctcttattc ggtatcccta agcaaaaaga cgcttgcggc agtgaatcaa
     2701 tgaataataa tgggatcatt caacaagcga tcaaaaaaat taaatccgtt aatagtgaca
     2761 tgttagtgat cactgatctt tgcttttgcg aatacaccga tcatggtcat tgcggggttc
     2821 tggatggtag tcaaattaat atcgcttcta ccttagagtt attaggaaaa caagcagtaa
     2881 gtcacgctga agcaggtgct gactgggttg ctcccagtgg catgacagac ggaatggttt
     2941 tggctatacg ccaggcattg gatactgcct catttcatga tattcctatt ttaagttaca
     3001 gtgccaaata ttgctcgtgc ctttatgggc cttttagaga ggcagcgcag ggtgcaccac
     3061 agtttggcga taggaaagcg tatcaaatgg atccggcaaa tagtgaggaa gctgtaaggg
     3121 aaacccgatt agatctggaa gaaggcgcag atatgattat ggttaaacca gccggttttt
     3181 atcttgatat tatttataaa cttaaacaac attttgctga tgtaccgctt tgtgcctatc
     3241 aggtcagcgg tgaatattcg atgataaaaa atgcagcaaa aaataatcta atcaatgaag
     3301 aacaagcaat gactgaaagt ctcattgcga ttaaacgtgc tggcgctgat tttattattt
     3361 cttattttgc taaaggcatg gctgagttat taaactctca aaattttatt taattatgag
     3421 tcaaataaat caaatcaaga actataaaac gactatataa agagaaagac ttgcctcatc
     3481 tataacaaat ctcatatagt aaatgggatt ggcattgcct aatcaactac aatctagaag
     3541 gagaaatcaa tgaatttaat gcttaaaggt ttatcggcct tagcaattag tctcggtgct
     3601 gcaacaactt ttgcagctcc agcttattta acaacccata acagaactgg tgaagaatcc
     3661 aatgcctaca tagcaggcag catcccctct ctttacccaa cagcagctta ctctaccaat
     3721 caagtgtatt ggaatttagt gagattggct tgctatggac atacaactaa tggtcaatgc
     3781 cctgccttaa ttaaaatggc aaccaataca gctaacccaa ttgatatcgg atacgttacc
     3841 atggatctga atactggcga tattactccc aagacgctat cggctaaagg ctattcgctg
     3901 agagtaatag gacctggaga ggctgaaatt actaaaaact agtcaattgt ctccatttgt
     3961 tttgaatagc ttatgtaggt tgaacgctaa tacaacctac ataatcaata catgtaaata
     4021 cttacgttta tcttaattaa gattaattat ctcagcacac gattatctaa acgttattta
     4081 tcccgacaca cataaaacta acagggctta tacaaaaccc caaggtcagt gcaaaaaata
     4141 tttcctaatt ttagcgaaga gattgggatt tttcataagt tctgactaat aaaaaagcca
     4201 cgcatatcac gtggcttcta acaaggtcaa gagaaagctt tatttagtcg gtgctggagg
     4261 ttctttatca atagtccaag ttgcttgcac acctactaaa ttggcacctg cagttccaga
     4321 tgctgtaacg ttatatctgg ttgttgcagt aaacgcatca gtcctgttaa taactggatc
     4381 acttgaagta aataaatggg tatatccgat atcgagaccg atattattcg ttgcctgata
     4441 atgagcgcca gcagataaag cccaacgatc tgtatccggt aaacgcacgt ctctgtcaat
     4501 attgttcgta ggtgttggat cataaccacc gcccacacga agcattaatt tatcattaac
     4561 atgataattg gcaccaatcg ctacccgcaa agtatcgtta taattctgcg gtgatatgct
     4621 tatcgccttg gcttgggaaa catttccatt atccgctggt cttccggcag ctgcattaga
     4681 gagttgtact gttttataac tactccatgc agtataaact aaagagccta acagtgcaag
     4741 tttgtcattg acatcatgat aagcactaat agtcactaca tcaggaaaat caattgagtt
     4801 actaaataaa tcatcgcttt gaaatatgcc aggtgccggg aacaaaaatg gcgctaatat
     4861 attacctgga cttgccaatg gccctgttag acgactgtag ccatagaatt catgccgcat
     4921 tgatgactga tagtttgcac cgagacgagt atgattatcg ttaaacatgc ccagaacacc
     4981 cacatgaaaa cctactccaa aagagtgtcc tttattgtaa gtcagtgaat cggctaacat
     5041 ggggttaaaa ggtggcaaaa cattcagtat gttaggtact ccaagtatac gattgtattt
     5101 aactctagca tattgcaaat ccaaaccagc ccccaaagca acgtgctctg ttaatctacc
     5161 accaatttcg ggtgatatat tggtggtaat taactcagta aaagtggctt gatagcgcac
     5221 tggagaatct gggccccaat ctgttgccaa tccaaatggt gatacaacac ttaacccaaa
     5281 cgtagcattt tcacccaatg gcaaggcata atgaaacgag ggcacaaaag catcctcagc
     5341 cccattgata ttattgaaaa cctcagaata aacaaaagaa gggaaagggg gcggattaat
     5401 tgttgagtaa gtactaactc cgcttaactt ggcagttggg aatacaccga cacctgcaaa
     5461 tacagcttgc tgatcgcgga ttaataccaa acctgctgga ttataccaac ctgtagaagc
     5521 atccgctgct tcagcagcca caccggcagc gaagttacct gttgtatatc cagctccttc
     5581 ggtatacaaa gaaaatccac ctgcgtttaa tgtaccagta gctagaactc cgagaacagc
     5641 agtactcact agcgttctta agggtttgtg cctaattttc actccacact cctttcgttt
     5701 aataacatcc caattcatgg taataaaaat gataataaat gaatttataa tgatttaaaa
     5761 cggtaaaaaa aaattatttc ctattttctt aaatttttat aaaagtttta atcataatga
     5821 gttaatcaag ttaattatgg taaaatcaaa ggcatatgat aaatttttat tcgagcctac
     5881 ctttcccaac ccatgctttg tagactcaac tcgtgacttg ggttgtcttg agaaaaatct
     5941 tttttaaatt ggggcttaat ctacttgtac aaatacttta gtccatgcta aggtgtatta
     6001 attttgagcg gaatactaat catgcaacaa gtagtcatca aatttggcgg cactagtgtt
     6061 tcaaccagag ccacttggaa taatattgct gcaattaccc aaaaacatct gaacacaggt
     6121 gttcagcccg ttatagtctg ttctgcttta actcaaattt ccaacaaact tgaaaaagcc
     6181 atagaggcag cccttcttga cgagcatcat agccttttca atgacattca gagcagtcac
     6241 ctgaatctgg cagaacaatt agaagtaaac catcaattaa ttgctaatga tttgcaccaa
     6301 ttacagcaat ggctaaccgg gatctcctta ctcaaacaag cacctgcaaa aacacatgcg
     6361 caaattttaa gtttgggtga gctgatgatg acccgcctgg gccatgcctt tcttgaaaaa
     6421 caagggatta aagtaaaatg gtatgatgcc agagaattgt taacaagcac accaaccctt
     6481 ggtggagaaa caatgaacta tctctctgcc cgttgtgaga gtgaatacga ctcggcattg
     6541 gtagaaaaat ttctctccag tggggctcaa gccattatta cccaaggttt ttttgctgcc
     6601 aatcctcatg gtgaaaccgt tttgcttggt cgaggaggct ctgatacttc agcagcacta
     6661 cttgcgggaa aactacaggc cgcttcttgt gaaatatgga ctgatgtccc aggcatttat
     6721 accgccaacc cgcatcaatt accccacgcc cgattattaa agcaattaaa ttatgatgaa
     6781 gctcaggaaa ttgcctcaat gggggccaaa gttttacacc ccaattgcat accacctgtt
     6841 cgaaaagcca atatccctat ggtagtcaaa tacactcacc tgcctgaaca ttcaggaaca
     6901 ttgattacca aagacattga tgaatcggca cccttaataa aatccatcca ggttaagcac
     6961 agcatactgc taatttctat agacactttg aatatgtggc aacaagtagg atttctggct
     7021 gatgtatttg ccgcctttaa aaagcatggg ttttcagttg atttgctttc ttcttctgaa
     7081 ttcaatgtca ccctatccct ggatgttaat gccaaaatac atgatagacc agcaattaat
     7141 gccttgctcg acgatttaaa ccaatttgga cgcgccaaac tgatagaacc ctgtagtgct
     7201 gtcagtttgg taggacatca catccgaact gtattacctc atcttggccc ggctttggaa
     7261 gtatttgagg caaaacaagt atacctgatg tctctggcct cgaatgacct taatttgact
     7321 tttgttgttg atgaatcaca tgccgataaa ttatgccaaa gattgcatca tctactaatc
     7381 gaaagtaatc ctcaaatctt ttattactcc aaaagctggc atgaagaatt tggcaaaccc
     7441 aatgttcgcc caacaccttg gtgggaaatt gaaagagacc gcttgcttac aaccagcgca
     7501 atacattctc cttgttatgt ttaccatagt cccacccagg caactcgtgc aagacaatta
     7561 tctgctctgg aatcaattga tagcttgttt tacgctatta aagcgaatcc attcccaacc
     7621 attttaaaaa ctctggaaaa agaagggata ggttttgaat gcgtttctat tcaggaattg
     7681 gaattggtgt taaaactgtt tcccaatatt aaaaaagaaa gaattttatt cacacccaat
     7741 ttcgctccca aatccgagta tgaatttgct ttgcaaacgg gctgttatgt gactattgat
     7801 agcttatacc ctttggaaaa ttggcctgac ttgtttaaag accgcgaagt aatcgttcgc
     7861 atagatcctg gtacaggagc tggccatcac aagcacgttt ctactggtgg aaatgaatcc
     7921 aaatttggta taacacaaaa tgacataagc aagattctat cacttaccaa agtcaaccac
     7981 attaaggtca taggcttaca tgctcattct ggtagtggca tactctcaac ggatttatgg
     8041 caacaaactg ccgtgatgct tgcctcccta accgatcaat ttcctgaagt gcgttcaatt
     8101 aatttaggtg gcggtctagg tattgtagaa aaaccgggcc aacatcccat tgattttgct
     8161 gcgcttgacg cgcaattaat ggcagtaaaa tctcaatatc caggattagc aatctggctt
     8221 gaaccaggtc gcttctttgt agctgaaagt ggtgtcattc ttgccaaagt aacgcaatgc
     8281 aaagaaaaag gaaaagtaaa atttattggt attgaaacag gaatgaactc attgataagg
     8341 ccttccttat atggtgcgta tcatgaaatt gtcaatttaa ctcgactaca tgaagaaaag
     8401 gccgggtttg ctcatatagt tggcccaatc tgtgagtcgg gagatacatt gggttatgac
     8461 cgcttattac cagttaccag agaaggcgat gtgatattaa ttgcaaacac tggagcctat
     8521 ggtcactgta tgagttcaca ctacaattta agaccacctg ctcaggaaat agttctggaa
     8581 taatggcatg cggttaattg atagtgaaca aaggagccaa gcgacagatc ctgctgaatc
     8641 atttatagtc caggctcctg ccgggtcagg aaaaacagag attcttaccc aacgcttttt
     8701 acgtttgcta agcactgtgc agtctcctga acaaattatc gccttgactt ttacccgcaa
     8761 agcagcaagt gaaatgagag aacgaatcgt tcaggcatta catatggccg cgtcaaatca
     8821 accggctgtc agcgctcatc aacaaagcac tttaaatttt gcaaaacagg ctttacagcg
     8881 aaataatcaa tatcagtggg atttacttga gcaaccaaat cgcttaaaaa tcataaccat
     8941 tgattcattg tgccaaagca ttaacagggc aattccttta ttggaaaaac aggtggctta
     9001 tccagacatt tccgagacac ccgattctca ttatattagt gctgcacgtt gttgtatcca
     9061 gtatgctatt gctacgccag agtaccagga agcaattaaa accttgcttc ttcatgttga
     9121 taataaacag gaacgattaa ttcaattatt ccaggcactt cttagccaaa gagatcaatg
     9181 gttgccgaca ttatttctgg cacgtgaaca agaaaagtca acttttgagc aagctttacg
     9241 ccttattgaa cagcatgaac tctctcgtct aagaaatagc ctgccatcgg acttggctca
     9301 agaactagtg cttttgtcgc gtgaattagc cgttatagaa aacaaacctg attcaccacg
     9361 atttatatta agagaatggt atgagtttca aaaatgcgat cagcaaattg caggtgcttt
     9421 aagcaaatta ctcttaacta gtgacaatca attgcgtaaa tcgtttgatc atcatgtggg
     9481 attagttaaa aaaaattgtg caccaaatga atttaccaga ataaaaacag ccagttcaga
     9541 tttgtttgct aaacttcaag attgtcctga tttcctggac gcgctcctta aggtcaatca
     9601 attaccaact cctgaatacg acaaagagca atgggacgtt ttgcaagcac tctttttgtt
     9661 actgccatta ttggttggac atttacaagt ttcatttggt gaacataacg aagttgattt
     9721 tactgccatc tctcaacaag ccttggcagc tttgggtgat ttggataatc cgactgattt
     9781 ggcactctat ctggatcatt caattcatca tcttctaata gatgagtttc aagatacctc
     9841 cattacacaa tttgaattac ttaccaagtt agtccaagga tggttacctg gagagggaaa
     9901 aaccttattc cttgtaggcg atcctatgca atccatctac cgtttcagac aggctgaagt
     9961 tggattgttt tttcaggcga aagagcatgg cataggacca gttcgcctca aatcattgga
    10021 actaagatgt aatttccgtt cgacagaaac aatagttaat tgggtaaatg atcatttcag
    10081 taaaatattt tctcaacaag ttgatatcga atcaggagca gtatctttcc atccttctgt
    10141 tcatgtcatc gagaagaatg aatcaagcac tattcaggcc gtgcaattta aaaatcgtga
    10201 acaggaagca aggcatttaa ttgaaataat taatagtgaa ttaaaccgca accctgaaca
    10261 aactatcgca gttttagtga ggacaagaac gcaactccct gaaatcatta gcctacttcg
    10321 ccaaaataat atcccttatc agggaacaga catcgattta ctagccaatt tagtgcattt
    10381 acgtgacgtc tggtcacttg ctcatgccct gcttttccca ggaaatcgat tgtcctggtt
    10441 atcgatactc cacagccctt atgttggatt gggattatct gacatccact gcatagccca
    10501 atataattca agaaaatcaa tttatcacaa tttgctccat ttagataaaa ttcaggatat
    10561 aagtgaagaa ggcaggatta gagccaattt ttttattcaa atcatgaacc aggctttgtt
    10621 atgtcgatca catagtcgtc tatctgattg gatagcggat accttaaaaa atcttcatgt
    10681 agaaaagatc cttgataaag ctcagttaaa tgatctggag caattatgga tgttattaga
    10741 ccaatatgaa aaagaaggtc gaattcctga tatgaacgaa tttttaaatg aattcaataa
    10801 attgtatgcc caacaaacca ccccttcccg gttacaaatc atgactattc ataaatccaa
    10861 aggactggaa ttcgatacgg ttattctacc tggccttggg tcacaagcaa atcgtggtga
    10921 atctcccatg ttgcgctggc ttaagctgcc tacccaagac catggcaact tgttactggt
    10981 atcacccata aaaagtgcaa accaggataa ttgccctttg tacgattatc tggatcaatt
    11041 ggatgaagaa aaatccgcct atgaggcgca aagattactt tatgttgctg ttacccgggc
    11101 aaaatcaaga ttatatttgc ttgatcattc ggcaaaatcc tataaatctt cctttcgaaa
    11161 cttattaaaa taccaggaat tcattgcaga tcctgacgaa gcccaacatg aagaacaaga
    11221 attccctctg ccgaaattag tcaaactacc tatagagtat tatcaaaaac agattcccag
    11281 tttagttcaa tctcaagaga gttcatgccc agagatatct tcgggtattc ctcggctaat
    11341 aggcattgtg tcccatcaac tattacaatg gatttgtgat aaccatcccc aaacaattaa
    11401 tcatgtgcca tggaacttgg tacataatga acttaaagca ttaggattaa accaggaagc
    11461 ccaacaaact gctctatcaa tgataaaaat ccaaatttct caaatgtatg aaaataaaat
    11521 tggcctctgg attttatcca gacatgaaaa tgaacaaaat gaatatgagc ttctggttga
    11581 gtatgaaaat aaattggtta cccgtattat cgatcgtact tttactgaaa atggcacaca
    11641 atggatcatt gattttaaaa ccgggaaaga agatataaac accttacaga agcatcgcca
    11701 acaattaaat gagtacgggc gctatctgtc agattgcaca aaattcccaa ttcgatgtgg
    11761 attgtattat cttgcaaatg gacattggat tgattggcag tataaaatga gcgcttaaaa
    11821 acacagcagc tatgcaattg cccaataaaa tcaaatgaca ttatcttaag ctattcgtct
    11881 tactcatcag aaaagctata cccaaaatac atttctctca agtagatgca ttcatcaacc
    11941 cagcaaaaac gaatagtaat ggtactagtg attagccgag acctaagtta tcattaccta
    12001 tttattttca taccaggcta aaaaatgact attgaagata cagtaattaa gttaattggc
    12061 tcattaaagg atcctttgct tgatcttact ggcaaagaaa tgaatctaca gtacaaaata
    12121 acaactagca accaggctat gcatgttgtc ttgactgccg gatatcctac ctctttgtta
    12181 gaaacgagct ataaaccaat agcacagaaa atagttcaag atgaattccc caattaccaa
    12241 gttactattt ctattcagca attcataaaa gcgcataaaa ctcagttgac aggcaaagcc
    12301 ctgcgaggag taaagaatac tatcgcagtg gcctctggca aaggaggggt cggaaagtct
    12361 actgtcacag taaatcttgc agcagcattg gcaaaactgg gtgctcgagt cggcattttg
    12421 gatgcggata tttatggacc aagcatacca ttgatgcttg gtgaaacaaa acctgtacaa
    12481 gtaaaagata attgctacat accggttgaa gctcatggca tgcaggccat gtctattggc
    12541 tacttgaccg atacaaacca ggcattaatt tggagaggtc ctatgctcgc caagtcttta
    12601 atccaaatgt tagacatcac tctttggaat gaattggatt atttattcat agatctacca
    12661 cctggcactg gagatattca actcactttg gtacaaaaaa ttcctttaac ctctgccata
    12721 gtagttacta ctcctcaaaa tgtcgcaact ttagatgccc aaaaggcaat aaccatgttt
    12781 tccagaacag gcattgacgt acttggagta atagaaaata tgtccaccca catttgtagc
    12841 cattgtggtc atcaagaagc aatctttggt agaggtggtg cagctgcgct gtgtgacgca
    12901 tatcaatgta cattattagg tcaactgccg ctggacagcc atgtacgtag acattgcgat
    12961 gagggagttc ccacggcaac acattcatca aatcaattaa ctgacacctt tattaaaaca
    13021 gcgatgcgaa ctgcaattga gctcagcaag aaacccataa actatgctga cagatttcca
    13081 aagatagttg tggaatagaa gtaatagctt ctctactatg tattattccc ttcagatgag
    13141 tatataatac ataaaaattg agtttgccac ctcaaagata accaagctca caaaaaacaa
    13201 aaataaaaat gtcatgatta gattattaat aataacaatc agtgtattat gttcgggact
    13261 tacgatagcc aaatcaagtt accaaattga tttaatactc tttgcacaac cacaaaacgg
    13321 tgttaaaagc ataaaatcag attcgcctgt tcccctcatt cctgtcagtc caaatgctat
    13381 tcaactgcat tcggataata ataaaaaaca tatgccttac actcttctac cgcgatcgca
    13441 atcaaaacta agtgatcaac attatctttt aagccgtaaa tcccaatttc aaatcctggg
    13501 tcattattcc tggagacaac cagcgaaaag tgacagtttg gttgcgctac ctaaaatcaa
    13561 ccataatgga tggcaaattc aaggtaccct caaagtgcgt caaagtaatt actatctatt
    13621 tgatgctgac ctgcagtgtt tgtctccagg taatcaggag acttctttta ctgtttcgca
    13681 aaaacaacgc ttaaaaggaa gcacaattta ttatcttgat catcctcagt taggaatgct
    13741 agttaaaatt cataaaatag cttcatgaaa gctcatccag ctaagcagga atttacgtat
    13801 aatttataat aatttctata ctaatttgga ttgaacattt tttgttatta attcagcaat
    13861 tgcattaaaa caaacttgtc gtggcgttcc tgctcgccaa aataatccca agatacgaga
    13921 gggtgccggc tcagaaaaag gaatacattt taaatgatta gttgaggcag ttagtgtaga
    13981 caaagcgggc agtaaagtga cgcccattcc agcttgtacc attaatctca aggtttccaa
    14041 actggtggca gtaaaatctg caatgtcgtc agctttcgct gattggcata cggccatggc
    14101 ttgctccctc aggcaatgcc cttcttctag caacataatc ggctgatttt ttaaatcatt
    14161 gatattaact gatttggctt gggccaaagg atgagtattt gcgcaagcaa aatagaactt
    14221 ttcttcatat aaagtttgac atgaaaaacg cttatctatt ggaagtgcca tgatagcaac
    14281 atccagttct ccttgctcca atttagtaat taacctatga gtttgatcct ctattaacca
    14341 aactttcagg cgagggtact catttttaag ttcgggcatt accaagggca acatgtaagg
    14401 cgatacggta ggtataaccc ccaaacgcaa ttcacctgta aatggatctt ccgactgacg
    14461 agcaagttct ttcatttcat caatcaggat taatattttt ctagttcgat ctaataattt
    14521 ggagccttga tcagtcagta aaacctgttt gttggtacgc tcaaataaca caacacctaa
    14581 ttcttcttcc agctttttta tttgcatact caacgtgggc tgacttacaa aacacctctt
    14641 agcggcttca ccaaaatgct ttacatcagc caggatgaca aaataatgta aatctcttaa
    14701 attcataatt gctcaataat aaaaaatcta ttgtgacaca aattacaaat ctgcagtata
    14761 aaaatcattt ttcaaatttt acgaaataca tacagaatta ttgattccaa tgtaatagca
    14821 aatataattc atgaagaaat aaaaggtatt gcagattata aatttacaca aacataacaa
    14881 tgctatatat tccatcaatt tcaacataat acaaactact ttgaacaatt tatcggctat
    14941 taagaaatca aaaatcacca tttggttggt aggagtattt tttttattat tccaattctt
    15001 cttgcaacta tcctccggga tcattgtagg cgcaataatg cacgaagaac atatttcggc
    15061 actcagcgca ggacttttaa gtagcgcctt ttactatgtc tatacttctt ttcaaattcc
    15121 tgtcgggatt ctttttgatc gctacaatac ccgatatttg ttatcggtca atgcaactct
    15181 ttgtgccatc ggatgttttg tatttgcctc gggacataac ctcccgactc ttttccttgg
    15241 gcggttaatt attggcgctg gctcagcctt tgcctttatt ggtttaagcc atttgttacg
    15301 ccaacatttt cccttaaaac aatacgcttt tatgataggc ctatcagaaa cgttgggatt
    15361 tactgttaca gtattaggaa tgattggcat gggttctgtg atcagccatt ttggatggcg
    15421 ttattttatt aacatagcag gaatcattgg attcctgatc gccagtttat gctggttgtg
    15481 tatccccaat agcaagccag tcgtttcttt tccacatcaa tataaacaac aaattatccc
    15541 cattctaaaa aataaattag cctggtttaa tggccttttt gtttgtctgg aattctcagt
    15601 aattactgtt tttgccgcca tgtgggctgt tccttttttg caattaaaac tggattgcac
    15661 tatcaaaaca gccagtatac taacttcaat ggtcttactc ggtgctggct taagctgtcc
    15721 tttattgggt caaatttcgg tacatctcaa tagacgaaaa ccgctgattc atgcttcttg
    15781 tctgtcaact gcgttattat taatacttgt tttatattta ccaactcaaa ataagctatt
    15841 gataggtatt ttaatgtttt ctatggggtt aacctgtggt gcttatatgc tagcctattc
    15901 aatttctaat gaattggccc cacctgaatt cttgtccact tgtactggct tcaccaatac
    15961 cttggcaatg ctctcggctc ccttacttca accactcgtt ggattttttt tagatgtact
    16021 cagtaaccat aaaagccaat acagcttggc tgactaccaa ctggcccttt tgattgttcc
    16081 ttttgccatg ctattggcaa gcttatttac ttgcctcctg ccagaaaaat caaattagac
    16141 tatcccaaat tagtaaccgc ttgtactgct gactccttga tttgcttaaa agtttgtttg
    16201 gatataaaac aaaatccttt tggattttct tcagaaaagc ctgcaactgt tagaggaaaa
    16261 tcatcctctt gttttatggt attgactaat tttaacttca acctgggcaa acccacgcta
    16321 agtaaacatt gattatcggt tagggtttta atggaaacaa attgagacat tttgacatta
    16381 tttcctgaag ggtctcgcca acctgctacc gcagcccccg ttgcatcagg ttccttgaga
    16441 tttgcagaat aaaaaccatt atcattactt aatctgcaaa aaatagtgct tatatcatga
    16501 ggggaatttt gatccttgct aaaccataat ttgctgaacc atgccttatt acctgttaca
    16561 aactgttctt tattatctac tcccgtttgc acgtgcccat ttaatcgact ggtctgtacc
    16621 gcttgcaact ctaccccagg tcttaatacc tgtgtttcaa catctttatt atcctgtttt
    16681 aacatatcta ctttaaaatt aaatcctttt gcattttcga ctttcacgcc aaaaatccaa
    16741 ctgtcattga tcggattgta gcgcaaaaat gagcgcccaa cttgccagtt caactgagtg
    16801 gagctatcta ttttttcgtt ggcctcataa aacagaatta actggttggt ttggccgtca
    16861 attaatgcgg ttttagcata aaaatcagta ccttgccttt gcatctgaaa aaaatagccg
    16921 tatcgatcac cgctttcatt ggtgaccata cctgtaaata cccaacttcc aacggttggt
    16981 tcattcgttt gggctacttt gtcttcctct ggctcttcca ctgcaaaaac actgccactt
    17041 aaaaataaaa acatggataa tagcaataac caaattcgtt ttggcatcat attagtaata
    17101 accttgtaat tgttgatgtg accaaaatgc ttcccaaaag gaatcattca actttgcttc
    17161 aacgggtaaa taatataatt tatctgaact aaatgaataa cattttacag catccttttc
    17221 tgtcattata actggtagat cgatatcatt taaatcatga ggtttaaatt gatgatgatc
    17281 gggataagaa tatggattaa attttatacc taattgactt aaagtagaat agaatcgttg
    17341 cgggttacca attcctgcta cagctgctac ttcagaagta aacaaatcat tactgacttc
    17401 ttcctgagtt gacaatcgaa ctatgttttt aggaattaat tccatagaat aagtaccttc
    17461 tgcagcacct tgattaacaa taacaaaatc aacttgtttt aatcgtgaat caggttccct
    17521 taatggtcca gcgggtagac aaaaaccatt acccagtttt cgcatcccat ctattactac
    17581 aatttcaata gatcgcccca ttttataatg ttgcaagcca tcatcactga taataatttc
    17641 aaccgagtgc ttgtctaaaa ggtaccttac tgcttcattg cgcttaggcg caataaccac
    17701 tgggcaatta atttttcttg ccatcatgag cggctcatcg cctactaatt cagctgaatc
    17761 atttaatttt acttcgtatg gaaaatgttt tattgctgct ttgtaaccac ggcttacaat
    17821 ccctactttt aagccttttt gctgtatttt tttagcaatt tcaatgacca gaggcgtctt
    17881 gcctactcct cctacggtca cgtttcccac tacaataatt ggtatagggt acagctgttg
    17941 gcagaaacgc tgtaaatacc atcgtctggt tcgtataaca attctgtata accaggaaaa
    18001 tggaaccagt atccactgta aaaaatggtt accataccat atcctgttca caaaaaatga
    18061 cattaagcta ccacttcctc attaatcctg ccaaattgtt gcactttata caactgagcg
    18121 taatgaccat ccatatcaag taattcttga tgactacctt gctcaacaat acgaccatgt
    18181 tgcaatacga taatcttgtg agcatgctta atagtcgaca atcggtgcgc aatgattaaa
    18241 gtagttctac ctttcatcac ttgctccaaa gccgcctgaa tataatgctc agactcacta
    18301 tccagagctg aagtcgcctc atcgagtatt aaaattggag catctttcaa aattgccctg
    18361 gctatcgcta ttctttgcct ttgtccgcct gacaacaaca ccccattttc tccgaccctg
    18421 gtatcatagc catcaggtaa ttgcttaata aactcatcag cataggccag tttagccgca
    18481 gtaataattt gctctcggct cgcatcaaaa cgaccatagg ctatgttgtt agccaaagta
    18541 tcattaaaaa gtgtcacgtt ttgactgacc aaggacatct gcttgcgtaa actttccaga
    18601 ctaagctgtt gaatgggcat gccatctaaa gtaatcatac cttgactcaa ttcataaaag
    18661 cggggtaata aactggctat ggtcgtttta ccacttcctg aatgcccaac cagagcaaca
    18721 gacgttcctg cttcaattac aaaattgaca tcatgtaaaa tattttgacc ctgtcggtat
    18781 gcgtggtata catgcttaaa ctcaatttca ccacgtatct tttcttttaa tatcaaacca
    18841 ttgtttcttt ccaaaggcaa atcaagcaaa ttaaatacac tttctgcacc agccaagcct
    18901 ctttgtatgg ttgcgttaag agtagtcaga gttttcatag gttttattaa ttgcaacatg
    18961 gctgcgataa ttgccaaaaa agaaccggca ctgatagtaa tgacagtaga taaatgaatg
    19021 gcagccataa taatcgtggc aatcccgata gcgatcacca attgaacgcc tgaaacatta
    19081 atcgccttgc ttatggcaac tttcatatcg tttttgcgtg aatattctgt tgctttatta
    19141 aattttgtta tttcataccg ttcaccacca aagatgcgaa ccactcgata accctcgatt
    19201 gcctcactgg caatttctgt gacttcgccc atggtttttt ggactttgtg actaattcgt
    19261 cttactcttt tattagtata attaacaatg attcccacaa aggggatagt taataaaaac
    19321 atcaaagaca gctgccaaca tataaccatc ataacggtca gcaaaccgat gaccagacat
    19381 atattttgaa taaaatctgt tagggcatcc gcactgactt gtgccacttg ctcaacatcg
    19441 tacaaaattt ttgaaagaag ctgacctgat gtggcttcgt cataataatc cgccggtaag
    19501 tgaataatat gagaaaatac ggtctgcctt aatactttaa ctactgagcg agccacccat
    19561 gtcatacaat aactgcctaa agaactgacc aatcctctca gagtaatacc gattaaaaca
    19621 atcagcggga tttgtttaac aaaattcaga tcaatagtaa taaagctttt atccaaaaaa
    19681 agcttggtca tataggtaaa gccagcgtca atgcccgaat ataggatatt cgccaatact
    19741 cctaataata aaactggcca aaaaggtttt acatagctta acaagcgttt gtatagcaga
    19801 cgggatttaa tagggaggtt atttttcatt aatttagaag ttaatcttgt acgcaaatgt
    19861 tatgaattgt aactcttatt ctgtctaagc gccaatgttc tatcatgaag tccacctatt
    19921 ccatagttag ttttattctg gtgtaggcac aacgaaatcc agccttttga acgttcaaat
    19981 ctgaaactgt acctggttga gtagttacgg tggcgaattc caaaccatac tgcttggcca
    20041 gattcagacg agcaaacaat aattttttct gaaggccttt ccctcgataa agaggtaatg
    20101 tgctggtaac gcccaaatca caagcatcac catgaattgc tattgtggcg ccagcaacca
    20161 aattaccatg atcatatgct gcaaatgcta aaactccttt tgcactggca tattggtaaa
    20221 attgttcctg agcctcaaga aaaccaaatc caatagctac tctttttgcc cactcttcca
    20281 attcagactg cttcacttct cttataataa acccatcatc ctcggagtga gcaaccagat
    20341 ggtaactctt taaatcaaga actgaaacat tatttaactc agttatgtag taacctcttt
    20401 ggcttaaaaa attaattaaa tgatttccca caaaaggaca taactcaata tcgacacgat
    20461 tatggcctaa cgatttataa aaattttcta tagcttctat ttcagcttta tattggttag
    20521 gctctgtttt aaatccccag cctactacct gggatagaaa tgactcaaaa ccagaaaaac
    20581 aagctgctcc gccgttgatt tccagaattt ttccttgtga gtaccgcttt gtcacctcaa
    20641 tgtgactctg cttgatacac ccttccatgc gaacagccaa ttccttagta atatacatcg
    20701 atttcctcac caccaggatc agatagcatt taaacctgaa acaatatcgt gaatcaactc
    20761 cggtccctta taaactaaac cactgtatac ttgtaataac gaagctcctg cattgatctt
    20821 gtctttggcg ctctccaaac tgtcaatacc acccactcct atcaatgtca catcattacc
    20881 aacatattgt ttcaacaagc gcaagcagcg agtagataat tccatcagtg gcctaccact
    20941 taatccgccc tgttcttcag aacaaggcaa attttttacc atttcccgag aacaggtggt
    21001 attcgttgca ataataccct cgattccata ctgtaaaatg atatctgtca tttgttttag
    21061 tgtttcatct gtttcatccg gagatacttt caccactaaa ggcacatggc gtccatattg
    21121 atctgctaat tttatctgtt ctttttgtaa ctgggtgagt aattcagcaa aataatctcc
    21181 ttgttgtaac tggcgtaaat caggggtatt gggagaggaa atattgatag tgacatagga
    21241 agcatgatca tatactttac ggaagcaata aagatagtcc tctgcagctt gatttaaatt
    21301 agtctcttta ttttttccaa tatttatccc taatattcct ttataatttg ctctttttac
    21361 attttctacc agtacatcaa ctcccgaatt gttaaaaccc atcctgttga taatcgcatt
    21421 tgcctctgca atacgaaaaa gccttggttt gggattacct gcttgtccct taggtgttac
    21481 tgtccctaat tcaatgaatg aaaatcccaa tttggctaat gcatccaaat gttcgccatt
    21541 tttatctaaa ccagctgcca gtccaacttg atgagggaat acaagaccca tagcatgaac
    21601 aggttgaccg gctattttcc gaaaataaaa accagggagg taatgtaata aggataatgt
    21661 taatgaatgg gctttttctg catccaatct gaaaagtaaa gggcgcagca gtgaatacat
    21721 aaaattactc ctaaagcagg gagaatcatt aataaagaac aattgaggta tgataacatt
    21781 tttcgtgatg attttcgtac tgacgattaa taggaagcga aataaaatta attcagataa
    21841 ttttttatgc atctgcccag ataaatagcg aaatgacttg ctcattatga ttgcaaatag
    21901 tcctgttgca taaacgagct tattctgctg ataggctatt ctccctgtct aaaagggaaa
    21961 atcataggcg cgtctctatt aatcgttggt ccactcataa taaagctaga atcttaaacc
    22021 acaaaaactg tattcctctg cagtattaat atgttcttcc ttaacaccag tatcactctt
    22081 tgatgttttt ggacctcttg atttttgttc gtttaaatca gaattaatca cttcttcatt
    22141 agcactcaat ttatcttttt ttaatccatt tagaattttt tgatgagaac cctgatcttt
    22201 gcattgcctc tccatcgagc ttctgattat tggctcaaca ggatgatctt gcgcctgatt
    22261 atcagctata aaatatgcag ccagggtagc agcaaacact cctgtagcaa agcctcccaa
    22321 ggcaaacata agcgcagtaa ccgctggtaa cgcaaaaatc ccggataatc caattgacaa
    22381 tggaactaaa acgatggcta gagtcattcc tagcaaaaac acaggaatta tagagagaga
    22441 aactacaaaa tctgtagcaa gccaacccaa taaagcagtc gctcctgaac tagcaaacga
    22501 agtcaaaccc aagcttcccc aaaatttcat gaaaccaaat ttttccccgg taacattatc
    22561 ctcattctgt tgagataaag tgttttcacc ttccaaagag ttggattgac actgtaaatc
    22621 atcatgacta atcagcaact cagatttatc ttttgttaat tcattgatca gaccatttcc
    22681 attattgttt tgtggcaaac tgggattatt ttttaaccga tgcgcaaacg ttgcagcaac
    22741 cgctccaaga aacaatcctg atacaacaag tagtaaacca ggtaaaataa ctggtgaagc
    22801 caacacagcc ggagcggcgc tcataaggag cataaaccct atataaaaac ccaaactgaa
    22861 ggagtcaacg ataacagcag ctaatgtcat ccccgacgcc aaccataaga taattgcagc
    22921 tgctcccata cttaataacg cagcaatcat gtgagatctt aaggaaggtg taaactcact
    22981 gtttttctta tttgatactg ccattttttc ctccctgcat accagtcact tactttaaaa
    23041 aagtaatctc aattaaacaa cacattgttg cgcccaaact cttagcattg cattacctga
    23101 tttatgcaaa tgaaaagcag cctccctaac ctccactcca tagttctcct gttgcaagcc
    23161 tagtaaaacc ggtcctagaa attgtgaagt gttaccttct ctatcaatca atggaaaaat
    23221 tctcacttct ttggctaccc ttgctaattc acgaattgca atcaggtgaa aatcaaccgt
    23281 ttgatcgtca agatctgcaa aaaaataatg agaactcaga gcaaaatcaa aggtaaaatc
    23341 ggcaaatggt aaatgataat cggtaagccc caggtacctt ccatcagcct taccttgttc
    23401 ataatccgca aaaaactgct tcattccttc ttgtctttcc tgaagcaatt tatccaaact
    23461 accattacgg ctaaagtcaa attgatcctg atgctgccta acctcttcag ccatattcgc
    23521 aaaaatcatt tttgctttag caagcagcgt atctttatcc aatacgaata aagggtcaca
    23581 actcactact ccattttttt tcacttgagt ttgctggtaa ttaacagcgc taggaccgca
    23641 accgtattct aatagtcgtg aattctccac tccctggggt aaatcaaaca tctcacgata
    23701 gtcttcaatt ccatgccccc ataataccag ttttctcatt agaaatactc ctcttctaaa
    23761 tccatttgga ttacactttg gctaaagatt aggtgatttt tttacccagg taaagaatca
    23821 ttttaaccac tgccctgggt aaaggtgttt ttgagataaa aatttaaaaa gtttggagag
    23881 aaaagccacc ttgcgattga acaaatgaat atttaccatt caaattcttt caatttttcg
    23941 caataagaca tccgtatatt atcaaatgta tcagtgggtt aaaaagattg tcttggaaat
    24001 aaacaggaag gtgacaagta cgcttcattt gggtatagtc tagcgtattt atcaacataa
    24061 caggatgtca ctcatgtaca caggcttaga ctttcaactg ggcgaaacgt atgacatgtt
    24121 acgagacagc gtcaaccaat ttgcccaaac tgaaatagct cctttagctg cacaaatcga
    24181 tgagaaaaat acttttccta accatctctg gagaaaactg ggagaaatgg gattacttgg
    24241 catcacagta agtgaagaat atggtggtgc caatatggga tacctcgcgc atgccatagc
    24301 aatggaagaa atttccagag cctccgcatc agttggttta agttatggcg cgcactctaa
    24361 tttgtgtgtc aatcaaatct ttttaaacgg aaataatgag caaaagcaaa aatatttgcc
    24421 taaacttata agcggtgaat atattggtgc tttggcgatg agtgagtcca actcagggtc
    24481 cgatgtagtc agtatgcaat tacaagcccg ttctgctgga gacaaatata ttcttgacgg
    24541 cacaaaaatg tggataacca atggtcctga cgcggatgtt ttggttgtct atgctaaaac
    24601 agacaagcag gctggaagca aagggattac tgcctttatt atcgaaaaag gaatgcccgg
    24661 atttaaaaca gcacaaaaac tagacaagct tggtatgcgt gggtcaaaca catgtgaatt
    24721 ggtatttgat caatgtgaag tgccttctga aaatgttctg ggaacagtaa atcaaggggt
    24781 caaggtatta atgagcggct tggattatga acgcacaatc cttgctgccg gaccagttgg
    24841 aattatgcaa gcttgtatgg atgttgtatt accctatgtc catgaacgca agcaatttaa
    24901 tcaggcgata ggtgagtttc aatttattca aggcaaactt gctgatatgt acactgattt
    24961 gaatgcttgc agagcttatc tgtatgccgt tgccaaggct tgtgacaacg gtagggttag
    25021 tcgtaaggat gctgcaggag ttattttata tactgcagaa aaagcaacac aaatggcttt
    25081 acagtcaata cagattcttg gaggaaatgg ttatattaat gaatatccta caggacgttt
    25141 attaagggat gcgaagttgt atgaaattgg ggcaggcact tctgaaataa gaagaatgct
    25201 aattggtcgt gaacttttta aagaaacagc ataaggaatg agacaatgga acataatgat
    25261 gtcgtgatag tcgcagcaaa aagaacacca atgggagcta tgctaggcaa tttatctgca
    25321 ctttcctccc cagagttagg ggcagtagcg catatggcag cgttgtctca atcaggtatt
    25381 gctccggcag aaatcgatga agtgatcagt ggttgtgtat tacaagctgg tattggtcaa
    25441 gctcctgcca gacaggcagc aataaaagca ggcataccta attcgacagg agccacgacc
    25501 attaataaaa tgtgtggttc gggcatgaag gctgtgatgc ttgcgcatga tttaattaag
    25561 gcaggtacag ctaatgtaat tttggctagc ggtatggaaa gtatgagcaa tgccccctat
    25621 ttattatcca aagcacgagc aggatataga ctgggtcatg gtgaactgaa agatcatatg
    25681 ttccttgatg gattagaaga tgcctatgat aaaggacaat taatggggtg tttcgctgaa
    25741 acaactgcaa aacactttaa tttcagcaga gaacaacaag acgaatatgc cttacgctca
    25801 atgaccaaag cgctaaaagc aatagagaat ggtgcctttc gagaagaaat cgctcctgtc
    25861 accttaacta cccgcaaagg agatatcacg gttgacgtcg atgaagggcc tgagacagcc
    25921 aaacttgcca aaatacctca gttaaaacca gcctttcaag cagatggtac agtcacagca
    25981 gccaattcaa gttctatttc agatggcgcg gccagtttaa ttttaatgag tgcctcaaac
    26041 gcagaaaaac gtggcattaa gcccttggca aaaatcatag cacatgccag ccattcccaa
    26101 gccccacaat ggttcacaac cgctcctgtt gatgcaatcc gtaaggtaat gaacaaagcg
    26161 tcatggaaac aagatgatgt ggatcttttt gagattaatg aagcgtttgc agtagtagcg
    26221 atggcagcaa ttactcagct tgaattgaac ccggaacaag tcaatataca tggcggggca
    26281 tgcgctttag ggcatcccat tggtgcttcg ggagctcgca ttctggttac tttaatgcat
    26341 gcgctaaaac accagggtaa aaaacgcgga atagcttctt tatgtatagg cggtggtgaa
    26401 gcaacagcta tggcaattga gctaatttaa cggttaaaga tgacctgggt aaaaccttta
    26461 tttttaccca ggtttttata ttaaaactgg ttgctatttt tatttgcgac cagataatgt
    26521 tttacctatc aaaaccaact tggttataac aataataaag gatgtacatg ctaagacata
    26581 gtctcattta ccttctacta agtattctta ttgtcgtatt tgcaaaatat gcccatctta
    26641 tcgtagttta catcgatatg tttttcactt acgttaattt aaaattaaca cctatattca
    26701 gccaaacagg ttggggactg attatcagga aaattctagt ccttatgtta ttacccataa
    26761 tcatcgcagg aatcccagcg ctaatttacc gggcactcaa aggaaaagac atgccccatt
    26821 ttattgcgat tgtctggata atatggacca tcatagtctt gagtgatatt ttgattcggt
    26881 aaggataaaa aatggctaag ttaactactc aaatcaatac cagcagtcag gaattcaaaa
    26941 ataatcaggc gaatatgcaa gcattggtta ctgatttaag agaaaaaata catcaaatct
    27001 ctctgggtgg agatgaaaaa gcacgtacca aacatcaaca acaaggcaaa ctacttccga
    27061 gagaacgctt acaccagttg ctggatccag gtagtccttt tcttgagcta tctcaacttg
    27121 ctgcctacca agtctatgaa gacacaatac ccgctgcagg aataatcact ggcattggaa
    27181 gagtggcagg aaacgaatgt gtgattgtgg tcaacgatgc cactgttaaa ggtggaacat
    27241 attatcctct gaccgtaaaa aaacacttaa gggcacaaga aattgcattg ataaaccatc
    27301 ttccatgcat ctatctggtg gattcaggag gtgcctttct accgcttcaa gatcaggtct
    27361 ttgccgataa agaacatttt ggacgtgttt tttataatca agcgcaaatg tcagcattaa
    27421 atattcctca aatagccgtg gttatgggat cttgtacggc gggaggagcc tatgtaccag
    27481 ccatggctga tgaatctatt atggtcaaga atcaggcaac catattcctt ggaggccctc
    27541 ctctcgttaa agcagcaaca ggagaagtaa tcagtgcgga agaattagga ggagccgaag
    27601 ttcactgtcg ccactcagga gtatctgatc attatgcgga aaatgacgct cacgcgcttc
    27661 atttagcgcg agtcgccatt agcaatttaa atcgtaaaaa accagattcc attcatagag
    27721 tggatacagt tcctcctctt tatgatagcg aagatcttac cggaataatt cccactgacc
    27781 ctagaaagcc ttttgatata agagaaatta ttgctcgagt ggtagatggt tcagagtttg
    27841 atgaattcaa agcacttttt ggaacaaccc tggtttgcgg ttttgctcgc ctatatggat
    27901 atcctatagg gattattgct aataatggca tactttttag tgaaagtgcg caaaagggca
    27961 gccatttcat agagctgtgc tgtcaacgta aaataccact tgtattcctg caaaatatta
    28021 caggatttat ggtgggctcc aaatacgaag catcaggtat tgcaaaacat ggagccaaaa
    28081 tggtgacagc agttgccaat gctaatgtac ccaaatttac catcattgtt ggtggaagtt
    28141 ttggagcagg taattatgcc atgtgtggcc gggcatatgc tcctcgtttc ctatgggcct
    28201 ggcctaatgc acgcatctca gttatgggtg gtgaacaagc agcgaatgtt ctcgcgcaaa
    28261 tcaccagaga gaaatatgca aagcaaggaa aagaatggtc actcgaagaa gaagaacaat
    28321 ttaaaactca aatgagaagt cagtatgaaa ctcagggaaa tccttactat gccagtgcca
    28381 ggctttggga tgatggggtc atcgcgcctc aggatacacg caaaatactc ggacttggat
    28441 tatctgcagc cttaaacgca ccgattgagg atactcgttt cggtgtattt cgcatgtaaa
    28501 aggaaaggaa caataggcat gagtgattta ttatatgaaa ttcaagataa agtaggttta
    28561 cttactatga atcgaattag caagcataat gcgttcgata atcaactgct gactgaaatg
    28621 agaatacgac ttgactccgc aataaatgat actaacgttc gcgtcatagt acttaaagcg
    28681 aatggaaagc atttttcagc tggtgctgat ttgacctgga tgcaaagcat ggctaacttt
    28741 actgaagaag aaaacctgga agacagtctg gtattaggta atttaatgta tagcctcagt
    28801 caaagcccaa agcctactat cgccatggtt caaggttccg cttttggtgg tggagctgga
    28861 ttggcagcag catgtgatat agccattgca tctacttcag cacgtttttg tttttctgaa
    28921 gtgaaattag gtcttattcc agcagtaatt agtccctatg tagttagagc aataggtgaa
    28981 cgagcggcaa aaatgttatt catgagtgct gaagtttttg atgccacacg tgcatattct
    29041 cttaatttag ttcagcactg cgtccctgat gatacgttgt tggagtttac tctcaaatac
    29101 gcctcgcaaa tcagtaataa tgctccagaa gcggttaaaa actcaaaaca actggcacaa
    29161 tacgtcgcta ataaaaaaat agatgaagaa ctggttcgct acactgcttc tctgattgct
    29221 cataaaagag tatctgatga ggggcaagaa gggcttaaag catttctcaa taaagaaata
    29281 cccaactgga ataaaggttc tggacatgtt taataaaata ttaattgcta accgaggcga
    29341 aatagcctgt cgaatcatta aaacagcaca ttctatgggt attcaggcta ttgccgttta
    29401 ttcagcagca gatagaaaca gccttcatgt gcgattagct gatagcgctt attatattgg
    29461 agaagcgcca gctaaagaaa gttatctgaa tattgaccat attattcagg cagctaaaga
    29521 aagtggagca caagccatcc atcctggcta tggattttta tctgaaaatc ctgattttgc
    29581 caaagcctgt gaacaagcag gtattgtttt catcggtccc tccattaaag cgatggaagc
    29641 gatggcatcc aaacaattag ctaaacaact tcttgaaaaa acgaaagtcc cacttacacc
    29701 gggctatcat ggcgttgaac agtctgaaga aaaattgttg agcgaagcta aaaaaattgg
    29761 atttccagta ctgattaaag ccgctaatgg tggtggtgga aaaggaatgc gagcagtaca
    29821 tgatgaaaaa gagtttcatg atgcattagc aggagccaaa agagaatcta tggcctcctt
    29881 tgcggatgat actatgataa ttgaacgatt agttcttaat cctcgtcatg ttgaagtaca
    29941 gatcatggcg gacaatcatg gaaatgttgt acatttgttt gaaagagatt gttcaattca
    30001 gcgcaggcat caaaaaatta tcgaggaggc ccctgctccc aatttatcgc ccatgcttcg
    30061 gcaaaggctg gcagaagcag cctgtgaagt agcccgctcc atcaactaca gaggagcagg
    30121 aacagtagag tttttggtag acggtgaaga taaattctac tttatggaaa tgaatacaag
    30181 acttcaagta gaacatcctg tcacagaaat gattacagga ctcgatcttg tcgcttggca
    30241 gataagaatt gcagcgaatg acacattacc tttgcttcaa aatcaaattc aagctcaagg
    30301 ccatgcaatc gagtgccgta tttacgccga agatccttat cagggtttta taccctctat
    30361 tggtcaactt caattcttaa aagaaccctc tggcgatggc atacgcattg atacaggtgt
    30421 tactttgtct tcagaaataa caaggtatta tgatcccatg atagccaagc tgattgcctg
    30481 gggacataat cgtgaagaag cattacaaag gttggagcgt agcctgtctc actatgacat
    30541 tggcggggta aaaacaaata taccgtttct acgagccatt tgccgacatg taaaatttaa
    30601 agaggcaaaa ttaagtactg attttttaga gaaagaaaac ataaatctgc ctaaaccaga
    30661 taatgagtta ggtatactcc tggccataag ttgtgattat ttaggtatgg ttaatcgcac
    30721 tacagatccc ttgttacaag atgcatttgg ctggcaaatg catctttcca gccattgggt
    30781 atggcgttat caattgaact ccacgataat tgaagctcag attacaccta tagatagtaa
    30841 aaaatttaaa gcaaaaattg aaaacaagga aatggtcatc catgccagat atgaccttga
    30901 ccagcttatt attgaaattg atcaaaaatc ggtgaaagcc agagtagaaa acaaagaaca
    30961 tcatttgatc ttctatactg acaaaggcca actttcaatc gaacgcttct actggagcaa
    31021 actggatgct caaacttctg cccataaagg ccaattaacc gcgccaatgc ctgccactgt
    31081 cgttgctatt ttaaaaaata ttggtgaaca ggtaaaagcc ggtgaaagct taattgtact
    31141 tgaggcaatg aaaatggaac acaccattca tgctccaatc gatggtatat tatcagatat
    31201 tttttattct gtaggttccc aagtgagtga gggagcagaa ctgttagcac ttagcgaatc
    31261 ggatacttaa gagaatattt atggactatc ctcagcatgt gacaataatc gaagtaggtc
    31321 ctagagatgg cttacaaaat gagccatctt ttctccccag tgataaaaaa attgaattaa
    31381 tcaatttact cagtcagaca gggctgaaag agatagaagt taccagtttt gtttctgcaa
    31441 aagcaattcc acagctagct gacaatgaag aagtatttca atctattaat aagactccct
    31501 ccatcaacta ctctgccctg gttccaaacg aacgtggaat gcttaaagct ctggaaatgg
    31561 gagtacaaaa tattgcagta ttcactgcag caagtgaatt attcaatcaa cgcaacatta
    31621 attgttctat taaggaaagt atagaacgct ttaaacctgt tttagcatta gcaaaaacta
    31681 atcaaattag agtgagaggc tatatctcct gcgtattggg ctgcccctat gagggatata
    31741 tacaaccgag tcaggttgtt agtgtcacta aaatgcttct tgatctgggc gtacacgaaa
    31801 tttcgttggg agatactatt ggcgtgggaa ctcccaggca aactcagttg cttcttgatg
    31861 ccatactacc catacttcca atcacacaat tagccatgca tttccacgat acttatggac
    31921 aagccgtagc aaatatttat gcctcccttg agtatggagt taaccgcttt gacagctctg
    31981 ttgcaggtct tggaggttgt ccatatgctc gaggtgccag cggcaatgtt gcaacagaag
    32041 atgtacttta tctgatgcat ggattgggta ttgatacagg tgtagatata ttcaaaattg
    32101 ttgctgccgg agatatgatt tgcaaagcat tagggcgaaa aaatcaatcc aaagtagcta
    32161 atgccatgct agccaatcca tgtaattagg aagtctcatc attattggct ttgataagta
    32221 ctaaaaatca gaaacttaaa gagaagtgcc cggttttgtg ctagcctata cttataaaca
    32281 aattactgat gaaattttcc agtgtaacac ctatcattca aaacagaact atgagagaaa
    32341 ttttcatgaa cgacaaagta tggacaccta aaaaccctga acaaacaaga atgtggcaat
    32401 ttatggattt tgccgcaaaa aggcattgtc aggtttttga aaattatcaa gacttacata
    32461 cctggtcagt aaagcatcct gaatcattct gggaaactct ttgccttttc tttaaattga
    32521 actttgatac accaccgcac caaattctga attattattc tgagatgatt gaagcccgct
    32581 ggttttcagg agctcgcttc aatttcgctg aaaaattatt aacccgtaga gataatcacc
    32641 ctgccctaat aagccttgat gaaaatgact ctaaaagtgt cattagctat gctgaattat
    32701 atgaaaaagt agcccaatgc gctgcaggtc ttaaagcagt tggtgtcgcc actggcgacc
    32761 gggtggcggc agtgatgcct aatacttctt acacaatcat tgccatgctg gcagcaactt
    32821 ctttaggggc aatctggtca tcctgctcac ctgactttgg tacccaggct gcacttgaca
    32881 ggataggaca aattgaacct aaagtactct ttatttgtga tggtcatcaa tatcaaggta
    32941 agaaacacag cagctctgaa aaggtcgtac aacttactgc tgcgattact tctctaagac
    33001 aggtagtaat ttgcccgaat attaatgagg aaattaatct cgaacaactg cctaacgcgt
    33061 cgtattggaa tgattttata aaacctgaaa aagaatgtga tttcatttct ctgccttttg
    33121 atcatccagt ttatattttg ttttcttcgg gaacaacggg taaacctaaa tgtatcgttc
    33181 atggcgcggg aggtacttta attcagcatc tgaaagaatt aggtcttcat tcagatatta
    33241 aatccacaga caatttatgt ttttatacga cgtgtggctg gatgatgtgg aactggactg
    33301 tttctgcgtt ggcattagga gctaccatca ctctatatga aggttctcca acctacccca
    33361 cagataatag aatgtttcga ctgatagatg aagaaaaagt cactgtattt ggaactagcg
    33421 ccaagttcat atccgccatt gaaaaagccg gggtaaaacc aaaaagtgaa tttcaattat
    33481 cggagttgcg ctgcatcttg tctaccggct cccctcttct gcctaaaaac tatgatttcg
    33541 tctatgaaca tattaaaaaa gatctgcaat taagttctat ttcaggagga acagatatta
    33601 tttcttgttt tgcgttggga aatcctattc tcccggttta tcggggtgaa ttacaatgca
    33661 ttggattagg tatggaagtt gcggtgtatg atgagcaagg ccattcaatc aaacaaaaac
    33721 gtggtgagtt ggtttgcacc aaacccttcc cttccatgcc agtctgtttt tggaatgata
    33781 aggatagaca agcatataaa catgcttatt ttgagcgctt cccaggggtg tgggcacatg
    33841 gcgatttcgc tgaaataact gcccacaatg gattaattat ccatggacgt tccgacgcta
    33901 tattaaatcc aggaggagtg cgtataggaa cagctgaaat ttacaggcaa gttgaaaaag
    33961 tagatgatgt tgttgatagc atagtcattg ggcaagattg gcaggatgat gtacgagtgg
    34021 ttttattcgt taagttgcgt gaggggaaaa aactggacga tgaattaatc aatctcatta
    34081 aaacaaccat tagaaaaaat gcctcaccac ggcatgtgcc agctaaaata ctacaagtcc
    34141 cagacattcc taaaaccctt agcgggaaag ttgtggagct cgctgtcagg caagttgttc
    34201 atggtcaatc agtaagtaac ttacagtctt tggcaaaccc acaagcatta gaatatttta
    34261 aagacaggga cgaattgaaa ggctaaaatt gcgtgaaaat agctatttcc agcacgaatt
    34321 cacattaaaa ttcatctcgt atcaaccaat agaaagggca attgcaagag ttgccctttc
    34381 atgtcaatac ttattattta aatattattt gaatgaaaaa aatttaataa tcctcataat
    34441 ttgaggaaaa ctcatttacc cgaatacttt aaaaccatcc cctaatttac aattcatttg
    34501 ataacttcct aatcgctcat aactacttgc attcagcaca gtggttttaa ttgaagcatc
    34561 accctcagaa tctaaattaa acttactgta atcaaacata ggtgtaccgc gagccaccgg
    34621 atcgtcaaga gtaaaatgcc tatctgaggc tgtaacacgg ctatcaccga ctaccataac
    34681 tgcattcgga gtaactgaaa ctattgcaga attcaatggc atttccgaag tacatttttt
    34741 taagttaatt acaaaagtga ttcgctttcc ttcagctatg gcatctacta tattttgaaa
    34801 agtttttaat tctactgctg cctgcgctgt gaatgagcac agggtaaaaa ctgctaaaaa
    34861 catctttttc atgaataata ttccttgtat taaaaccggc ttaaggttgc caataagaat
    34921 ttatcttgtc aatcctcaac caaaataatt attttcagac cgaatgacac cgcgtatctg
    34981 attaatgcga attatctctt tttatataaa ccaaaagcca accaataatc atgaaaaaca
    35041 aattgagata cctcaacaga attaagattt ttctttgttt atgaatccag tttgtgtata
    35101 ctt
//
LOCUS       DATNSN010000015        34848 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.015, whole genome shotgun sequence.
ACCESSION   DATNSN010000015 DATNSN010000000
VERSION     DATNSN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 34848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.015"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(56..1438)
                     /gene="mavV"
                     /locus_tag="VAH76_000595"
     CDS             complement(56..1438)
                     /gene="mavV"
                     /locus_tag="VAH76_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavV"
                     /protein_id="HEO1385244.1"
                     /translation="MKYAVTFCTTDQEVGSNPLWHSCLLLSKMDETSKLLEVVDNWGF
                     YGLPTTNRNNSWLDQLKIKMGLDVDLIGNHGMLRHEELRFLDQGYGLRGVTFELTKDD
                     FELLQKKCLQMATDQETAINEFVETQGIKGKPPEKTRIYPHEQFSRLIYATENIKAEQ
                     QGREARLKPFELRLSWGLFGPSLALSQNCKTQALSLLSYVLSKEQIERLTEYGEHPTV
                     PRRSGPMESIFLHSTGPLREHRKSSGDVVHYRDLKDPGVKLYWTVPLQELEALSEETI
                     KLLQIDGEYCEEVKAIVRKLQRLEWLLINSNLPEKYRPYKDELINKVVGCYKAFSFIE
                     PKQEEPKLGGWYGWALSLFSAPRSAEERTLQGKIKKAKSLFNSIYMAIVDNYQIDDNA
                     PMENENILPYEETGENRFENMVEAVASYLSRKDKINLCKIIGRTYCEDHVELEDNEGK
                     IALSPQPLAT"
     gene            complement(1846..5448)
                     /gene="enhC"
                     /locus_tag="VAH76_000596"
     CDS             complement(1846..5448)
                     /gene="enhC"
                     /locus_tag="VAH76_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhC"
                     /protein_id="HEO1385245.1"
                     /translation="MKSLVPWVCVLVASAGQQAFANDAFNAYRLGNYNEAAEPLIIKS
                     GQDAVAEYYLGRLYLYGYGQLKNNRVAMRYFTKSAEKGYLPAVLLMAKYSLLHEKNPE
                     EAARWFKQAAAVGDVSAQMYMAAAYLYGVGVKKNTDIARRYYIDAAKNGNPIAQYTLA
                     EYFLESKHAANKKLGIIWLNKAAENGDPKALTELGRFYVAGQMVAKDTAKGVELLNKA
                     ASEQYTPAMLELGKLALMQNQYDEAIQWLNKAEEQSTEATLELAHIYLQDKSPVYDPK
                     TGFLLVLKAAQNGSVQAQKELANLYQKGIGVAADEELSKQWTLKAAENEKKNAEPPVL
                     AQAALWLSNDKTDKLEETAYQMNGIFSPWNNPSALRNNSYNQAPKLETISRQDIFKPQ
                     FELTQPNSIPISNYYDALISKQVEFQSNQWAYPTYRLNPFVEALERANSPVVQKLNLP
                     VPYADANYDDSEVGSFDLMDIWTGDWKRTVNYMSVVNYLYFRAILGNAQTQFEIGQLF
                     QYGIGLMQDDASAIIFYENAAEQKHLGAEYNLGILYLKRGKDENDYQQALNWLTDSAF
                     KGNQRAQYVLARILRQGIVGPDGKEYIKANDEQAMAMLYLSAANDYGPAEYELAEYLA
                     RDYNNGLSVDVRKQKIALIRKLYQGAVKNKVAEALLPLAFYNAMDDNRQKQEQAFKVA
                     EEQAETGNANAALLLGMLYDRGIGITADPAKAMYWYQQAGQNPVSQFILGTYITEGKG
                     VAQDKEKGLDLLKQSADSQFSYADFNLAVLKQQSGEDFLPNLIESYKLGNSHAGIVLA
                     DYYLSQSSVPEQMNQAKEIYKGLADKGDQYAQLKLAYMLQKGLGAAPDLAGAQHWYTA
                     SAEQGNPEAQYLLGQFYQLGETGEPDYNLAKYWYQKAAKHLSKADVALGFIYETVDDN
                     YAQALKAYENAAAKGDMLGAYNLALMYEYGKGVPLNYSKALSLFKEASEKGAPEAMSQ
                     LAGMYFYGLGQPRNEQQALVWYKKAASLGNGNALYALGLLSETGVGVKLDFPDALRYY
                     QDASDKGNEKAMLALARMYHYGLGVEKDHKRSASIYQKLAQKQNAYAQYQLGTYYIEG
                     TAGERLPEKGRELLQQASENGSLQARRVLQRLDAQTQGKVSYVEPVLFNNVPKFDGQS
                     ADMIYFDALNEWNHGEEVLSRMILQRLVTQYPNFIPAKRAYEQLNQAKLMNNLG"
     gene            complement(5475..6041)
                     /locus_tag="VAH76_000597"
     CDS             complement(5475..6041)
                     /locus_tag="VAH76_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase IV"
                     /protein_id="HEO1385246.1"
                     /translation="MSKMSNGLMTASVLVLLLGYGGVIAQAAKSKIAKNSDEKTEKKD
                     AEKFPLGCTAVGYQQTLKVLTLLPGKEGALQSMYFIYNKLPQTVSLYQMHGSESLYSY
                     WLNHSIRANSWAVLATGEKEVKFICTLGDGKKSYGKILDCADAVKVCEYVNVKFGLNN
                     KGNFWIVDSNTRNGAVSQVVHYGIIPGV"
     gene            complement(6054..6761)
                     /locus_tag="VAH76_000598"
     CDS             complement(6054..6761)
                     /locus_tag="VAH76_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1385247.1"
                     /translation="MKKILLTTFMMSMVTALNAGHFYGAALCAYPQYECIKVTGGQSW
                     EKLFPDPVQRDIVQRVNRTYNYLWSGREIAVPKNLANINIFDVSPFPLKIDSENEKQI
                     IVDQEKLAWGAYDAQGNLVWWGPISSGSDKCSDSNKVCRTLTGIFRVFSKENVRCTSG
                     VFPIGRGGAKMPYCMYFHKGFALHGSDDIPGVRASHGCVRMFIQDAKWLNENFVELSS
                     ERNNFMGTKVIVRPLNK"
     gene            6933..9437
                     /locus_tag="VAH76_000599"
                     /pseudo
     CDS             6933..9437
                     /locus_tag="VAH76_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            9578..10237
                     /locus_tag="VAH76_000600"
     CDS             9578..10237
                     /locus_tag="VAH76_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385248.1"
                     /translation="MPFCFSNQPNILNNKLILKIVVLILFFSNSSFAKTINYPWTVSA
                     SLGYVNYQYVHEGDHITATGRFAIGRDLLMKGPFLFGLELGLQSGNQMRLQMTEEEVD
                     ALGGLLVQTTLKPMVDVLATIRITPEFTAPLFLQLKAGTAYRQWQMDRDTINDISQLA
                     GEIMAGCGYAITPNASITLSYQGLFGDKPNFTIDLESGIGHVQKIPVQHAILLGIVLM
                     I"
     gene            10405..11562
                     /locus_tag="VAH76_000601"
     CDS             10405..11562
                     /locus_tag="VAH76_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEO1385249.1"
                     /translation="MIHRNKVLNSILLILFTSQIAFAKSNPASQAYVDGKVSELKNEL
                     TNKINSIPSGPQGPKGDKGEAGPKGDRGEAGPQGLPGLKGDRGEAGPQGLPGPKGDRG
                     EAGPQGLPGPKGDQGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGPQGLPGPKGD
                     RGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGPQGLPGPKGDKGETGAVGPQGMP
                     GPKGEAGDDGQGVPAGGETGQVLAKSNDLDFNTVWVDPANSGIRRQLGDKALGGTVIY
                     VNALGTHGLVVANSDQVNSNTWWDAQDSITNPAHFDNEGKLYSDWRLPSRFELNLIYM
                     MRDELGNFLAGNYWSSIEKSSANSWVFNSKTGEIKDIAKSKTAAVRAVRAF"
     gene            complement(11586..13442)
                     /gene="uvrC"
                     /locus_tag="VAH76_000602"
     CDS             complement(11586..13442)
                     /gene="uvrC"
                     /locus_tag="VAH76_000602"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO1385250.1"
                     /translation="MNDLQLSAELALFLTKLPGEPGIYRMLDEEGTVLYVGKAANLKK
                     RVNSYFSKQNTGVKTRALVSQIKSIEISVTRSETEALLLESNLIKALRPKYNVLLRDD
                     KSYPYIHLSNHPDFPRIELYRSKKKPPSGNFFGPYPGVAAVRETIVTIQKIFKIRNCR
                     DSYFKARSRPCLQYQIKRCTAPCVHYISPENYKLSVEDAIRFLQGKCQIILDELAERM
                     KQAVSQLNFEEAAVLRDQIKNLRLIQEQQGVVQLRGDADVIAIEVRPGFACIQCVTIR
                     EGQVLNSQSFFPTVPYAVLDEELDANSLWQQTFEAFVGFYYLDTSERIPDLIITNQSI
                     TESRSLEYILSQRRGKSCKIQVNPRGVKSRWMDFAVNNLRISVAEYVSKHSTIKSRYQ
                     ALKQLLALDKNIERMECFDISHTQGESTVASCVVFDTEGPRPSEYRRFNIEDITPGDD
                     YAAMEQAVTRRFKRLIDAQLLPDVLIIDGGKGQVSIVKRVITSLGVEDITLLGVSKGP
                     SRKAGWEKLILVNENREFVLPEDSKALHLLQHIRDEAHRFAITAHRKKRQKTRVESTL
                     ESIEGVGTKRRQALLQRFGGLRELAKAPLEEICKVQGINEQLAKRIYEHFHP"
     gene            complement(13454..14113)
                     /gene="uvrY"
                     /locus_tag="VAH76_000603"
     CDS             complement(13454..14113)
                     /gene="uvrY"
                     /locus_tag="VAH76_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HEO1385251.1"
                     /translation="MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVK
                     THSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYL
                     TKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQEPQDSPFDLLSEREMQVMLMI
                     TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQD
                     D"
     gene            complement(14238..15056)
                     /locus_tag="VAH76_000604"
     CDS             complement(14238..15056)
                     /locus_tag="VAH76_000604"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEO1385252.1"
                     /translation="MEFSSRYVAHVPDAQGLVDYSAQENRIWNILFERQLKLLPGRAC
                     DEFLSGLQTLGLNPSTIPQLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPA
                     ATFIRSEEELDYVQEPDIFHELFGHCPMLTDRVYAEFVHDYACKVLTFPEQDWPLLQR
                     MFWFTVEFGLIKTPKGLRAYGGGILSSISETVYCVESDIPVRVLFDPVVAFRMPYRID
                     QLQPVYFVIDSYQNLYDFVLSDMGKFMDRARELGEFPPYFDVDPDNPNIHIRAC"
     gene            15496..16593
                     /locus_tag="VAH76_000605"
     CDS             15496..16593
                     /locus_tag="VAH76_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO1385253.1"
                     /translation="MTIAILSTGDEIIHGDTLNTNAHYIAHALCSEGLPLGLQVACSD
                     KENEIIDSLNFLTEHHDILILIGGLGPTKDDITRFALAKFTKEPLIQHADALDHIEKK
                     ASIAKISLNQGNLQQCLFPANARLFPNPYGTAMGCSYSWKGKVFILLPGPPRECLPMF
                     NQDVLPLLLQTSRHSNKQILRWRIFGLAESEIAQILEEALEGLDCQTGYRLETPYLEF
                     KVRCKKELVEKITSIIDPILAPHIISSLETKASDQLRSLIVKNNEPITIIDDVSGGLL
                     QSLLVRPENYHLLNFHNLNRTKLYFHCSGLENYWSQQAPQGTTKLIIHYSNQMQEGRE
                     THQIPYRSAMVVHYAAEWLSFRLFHLINQLH"
     gene            complement(16552..17577)
                     /gene="obgE"
                     /locus_tag="VAH76_000606"
     CDS             complement(16552..17577)
                     /gene="obgE"
                     /locus_tag="VAH76_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO1385254.1"
                     /translation="MKFVDEALIKVEAGKGGNGCLSFRREKFIPRGGPDGGDGGDGGS
                     IYFEASSDLNTLIDFRYTRQYKAENGQSGMGGNCTGKKGEDLTIKVPVGTMVYDADTG
                     ELLADISQPGVPVLIAQGGFHGLGNTRYKSSVNRSPRQTTPGSPGESRNLRLELRVLA
                     DVGLLGLPNAGKSTLIRAVSSSKAKVADYPFTTLHPGLGVVRVSPYKSFVMADIPGLI
                     EGAAQGAGLGHRFLKHLSRTCVLLHVIDIAPLDGSDPVADAKAILNELTQYNPDLLNK
                     PRWLVLNKIDMLPDEKEREEKIQSIIKGLEWKDKVFSISAIETKGTQELCYALMQLID
                     EMKESEA"
     gene            complement(17792..18070)
                     /gene="rpmA"
                     /locus_tag="VAH76_000607"
     CDS             complement(17792..18070)
                     /gene="rpmA"
                     /locus_tag="VAH76_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO1385255.1"
                     /translation="MAHKKAGGSTRNGRDSNPKYLGVKRFGGQFVNAGEIIVRQRGTR
                     FHPGPGVGCGRDHTLYALVEGLVQFTTKGEKNRKYVTILPEQREEAAS"
     gene            complement(18083..18394)
                     /gene="rplU"
                     /locus_tag="VAH76_000608"
     CDS             complement(18083..18394)
                     /gene="rplU"
                     /locus_tag="VAH76_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO1385256.1"
                     /translation="MYAVIKTGGKQYTVKEGDVLKIEMLPENVGNEIKFSEVLMLVDG
                     DKVTCGTPFVAKATVKAEVLDHGRHKKVKIIKFRRRKHHMKQMGHRQYYSQVKITAIG
                     K"
     gene            18637..19296
                     /locus_tag="VAH76_000609"
     CDS             18637..19296
                     /locus_tag="VAH76_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEO1385257.1"
                     /translation="MSTIQLEAQSRTDMGKGASRRLRRLENKVPAVIYGGSKKPMAIH
                     FSHNKVIKALETESIYSSVFDITVDGKVEHVILKALQRHPYKPIVLHMDLQRVSSKDI
                     LVKLVPVHFINEEQSPGIKAGGIVQHTMTQVEIRCQAKDLPEFIEVDMSKVGMDDVVH
                     LSDLKLPKGVQLTVDVTDGSHDAPVVSIHAAKVSSTELEETPEVPASAVPTTDQGESA
                     E"
     gene            19415..19984
                     /gene="pth"
                     /locus_tag="VAH76_000610"
     CDS             19415..19984
                     /gene="pth"
                     /locus_tag="VAH76_000610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO1385258.1"
                     /translation="MAIKLIVGLRNPGSAYEQTRHNAGAWLVTALAQRHNSHFKIDKK
                     MQAELTEIDINNHPCRLVLPLSFMNHSGQTTRIISQFYKIEPGEILIVHDELDLPVGR
                     IKLKTGGGHGGHNGLRDITAQLGTGVFHRLRIGIGHPGHKDLVHQYVLSRPSMHDRQQ
                     IYDAIDRGIAIIPIVLSGDMARAMNQVNA"
     gene            20003..21094
                     /gene="ychF"
                     /locus_tag="VAH76_000611"
     CDS             20003..21094
                     /gene="ychF"
                     /locus_tag="VAH76_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO1385259.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNVGIV
                     TVPDSRLDNLSDIVKPQQVIHATMQFVDIAGLVKGASSGEGLGNQFLANIRETDAIAH
                     VVRCFDNSDVVHVEGRVDPLSDIEVINTELALADMETLEKSLLKVGKNSKSGNKEAIF
                     ELKTLEKIKAHLDTGYPVRTLELTPEEEQVSKRLFLLTAKPVLYIANVDDNGYENNPL
                     LDKVKALASQENASIVALCAATEAELVELDEEDRQEFMVDLGLSEPGLNKVIRAGYEL
                     LGLQTYFTAGVKEVRAWTIRKGATAPQAAGVIHTDFEKGFIRAEVISYEDFIRFGGEQ
                     GAKEAGKLRLEGKEYIVCDGDVMHFRFNV"
     gene            21437..22552
                     /locus_tag="VAH76_000612"
     CDS             21437..22552
                     /locus_tag="VAH76_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEO1385260.1"
                     /translation="MSLLKKTREQDYSILVTTQDQQRKRHLLNVISIELLILGLLLII
                     TNLCLLRAWLIISMLIVGIFILLGNLILIKKNFNLALSGHILNILVLSIIIGGNIIVG
                     KTTMSYLGWFYVSPIVAFATLGLAGLVVYGVLAIGAMFLFVFEFYIPIYSISQEYVFF
                     LDNTNHLFIFALIFTVLYHFLVQNSQYELLLKEQNYLLIADKEKFHYLSHHDSLTNLP
                     NRPYFHAHLQTLLENTDTKKEAITLYFMDLDKFKPINDEYGHEFGDQLLLLTSKRLQS
                     CFRKNDFIARLGGDEFTAIIVHKPHDQIAEALTLRIEQEFEQPFVINGQTIHCTISVG
                     IANYPRNAKNAEDLLKWADKAMYDNKRKKYQTLKQTS"
     gene            22686..23654
                     /locus_tag="VAH76_000613"
     CDS             22686..23654
                     /locus_tag="VAH76_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO1385261.1"
                     /translation="MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGG
                     KRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSI
                     WGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
                     DDYFDVIRAKTALLFAAAACIGPILTNAPIEIRDSLYNYGLHLGNAFQLIDDALDYCS
                     DAATLGKNIGDDLADGKATLPLIHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQ
                     TEAIPFTKRIAAQEVDSALSALGILPDSVYKEALVDLAKFALERNY"
     gene            complement(23681..23896)
                     /locus_tag="VAH76_000614"
     CDS             complement(23681..23896)
                     /locus_tag="VAH76_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385262.1"
                     /translation="MLLQIRDFIYREGVVSTQQLSREFHLDLTALQPMLDLWVRKGAI
                     RKCQGGANCKTACFKCRTPPDYYQSVV"
     gene            complement(23877..26132)
                     /gene="feoB"
                     /locus_tag="VAH76_000615"
     CDS             complement(23877..26132)
                     /gene="feoB"
                     /locus_tag="VAH76_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HEO1385263.1"
                     /translation="MTHALLIGNPNCGKTTLFNALTNANQRVGNWPGVTVEKKTGEFL
                     LGEHLIEITDLPGVYSLVANAEGISQDEQIAAQSVIDLEYDCIINVIDACHLERHLYL
                     TSQLFELGKPVVVALNMMDIAEHRGISIDTEKLESLLGCSVIPIQAHKNIGIPALQQS
                     LLHCSQKIKPLKLSLSVVAQQVLDDLENQLISKGYKNSFAYYFSRRLAEGDTLIGEKA
                     FTESLLIKLQETEQNLDVLLADARYQKIHEIVTLVQKKHSDASEHFTAKLDKLVLHRF
                     LALPIFFAMMYLMFLFAINIGGAFQDFFDISTETIFVQGSGWLLQQLHAPNWVIALVA
                     NGVGKGINTTITFIPVIAAMFFFLSLLETSGYMARAAFVVDKAMRAMGLPGKSFVPMI
                     VGFGCNVPAIMAARTLDSERDRLLTVMMSPFMSCSARLAIYAVFVAAFFPSGGHNIVF
                     SLYLIGILMAVFTGYILRKTTLKGHASPLILELPAYHRPSLRRLLRETSMRLRFFVYR
                     AGKLIIPICVILGGLNAITWGGGISSGEANTDSLLSIIGQWITPLFAPMGIHQDNWPA
                     TVGLLTGMLAKEVVVGTLNSLYAQVGHVGEIAAAHFDFWGGIKAAFGSIPANLSELGS
                     ALWNPVSASAADSELSQSVYGIMSRRFDGAVGAYAYLLFVLLYIPCVSTMAVIRQEAN
                     KRFMWTSIVWSFVVAYATSVVFYQGATFLDHPQQSMVWILAMSLSLLFVLAVFRYSHY
                     GMGRQNAAANT"
     gene            complement(26129..26356)
                     /gene="feoA"
                     /locus_tag="VAH76_000616"
     CDS             complement(26129..26356)
                     /gene="feoA"
                     /locus_tag="VAH76_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter FeoA"
                     /protein_id="HEO1385264.1"
                     /translation="MQISELKQGDKVRLVSFGATDMQYRRRLLSLGITCGIEFSVVRM
                     APLGCPVQIEVRGTFLTLRKDEASQLVLEHL"
     gene            complement(26445..27527)
                     /locus_tag="VAH76_000617"
     CDS             complement(26445..27527)
                     /locus_tag="VAH76_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEO1385265.1"
                     /translation="MSLIEQYEAAIYRGEIDSDPEQREILEHMQRLAEDLQKKNDSWF
                     PWRKKHPIKGLYIYGPVGVGKTYLVDLFYQHIDEEKKARFHFHHFMQQIDAQLRRLQG
                     KKNPLQYIAKEMAKSIRLLCFDEFLVHDVAYAMILAELLQALHRYGVVLVISSNTHPD
                     ELYLNGVHRERFIPAIEIIKKDCEVLNLNQKRDYRLGRQPLLEAYLYPLNEQIQEAME
                     KQFALITREEDCKGFITVQNRDIPYIKYGARSIWFAFDVICNFPRSQLDYLEIADRFD
                     NVFLSDVYQLTESHTAQAIMFIHFIDVMYDRGIKLIISADVPAEELYVKGEMKETFKR
                     TLSRLIEMQSVDYLSRHPRRLVKEML"
     gene            complement(27524..28186)
                     /locus_tag="VAH76_000618"
     CDS             complement(27524..28186)
                     /locus_tag="VAH76_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385266.1"
                     /translation="MLFTDKLNTPGEHELMLEGLVGPLEAVLTVPEDADTRYVAFLGH
                     PHSLQGGTMNNKVVTTMARAFKELHIPSVRFNFRGVGQSGGHYDNGIGESEDMRKLVR
                     QWHSEQPQAQNIFAGFSFGSYVAYRTASHCEHALLITIAPPVHHYDYKEFEPLPHPWL
                     IIQGKEDEVVPFELVSNFASQLSQVLPVIEFVETGHFFHGKLLILKEKLMNAIRDQVC
                     LP"
     gene            28423..29211
                     /gene="panB"
                     /locus_tag="VAH76_000619"
     CDS             28423..29211
                     /gene="panB"
                     /locus_tag="VAH76_000619"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEO1385267.1"
                     /translation="MKIHDFKIKKQEQKKISMLTCYDYPSACIVAESNIDCVLVGDSV
                     AMAVHGHPTTIMATIEMMELHTQAVARGLGKQFLITDLPFLGHKSSQGHTVENVKRLL
                     QAGAQAVKIEGADKDTCQTISHLVNAGIPVMGHIGLTPQSIHQLGGYKVQGKNSEQAE
                     TLLQQADALEQAGCFAVVIECVPQGLAKTITDSLVIPTIGIGAGPGTDGQVLVWHDML
                     GLQTSFNPKFVKKYFRAKDHFIEALNSYVQQVQQMHFPANEHSF"
     gene            29223..29981
                     /gene="panC"
                     /locus_tag="VAH76_000620"
     CDS             29223..29981
                     /gene="panC"
                     /locus_tag="VAH76_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEO1385268.1"
                     /translation="MQIFHNLNEWIRFRNTLSPDLSLGFAPTMGNLHAGHASLFLASS
                     KENHYTVSSLFVNPTQFNNPDDYKHYPRTVDADLELMTQNGVDFCILPNENEIYTDGY
                     AYQVQENRLGQLMEGKHRPGHFNGVLTIVMKLFNLVKPTRAYFGEKDYQQLLLIQGMV
                     KALFMDIEIKSCPTVREKSGLACSSRNNRLTPSQREIADEFAKIFHQNKSSAMISKEL
                     EALGITVEYIEEFQGRRFAAVKIGDIRLIDNYLL"
     gene            29978..30511
                     /locus_tag="VAH76_000621"
     CDS             29978..30511
                     /locus_tag="VAH76_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1385269.1"
                     /translation="MIQTNWCVITGGPSSGKTTLINHLAELGYKTAPEIARFYINQLL
                     SHDKKTLQEVKHDILWLQRKILNIMLRRERNLPKDDLIFFDRGTPDSIGYFRFHHLDE
                     AHVVRACQYLRYKKIFFCHPLPVVPDTIRDEDDVTAKKISDYIYNAYVGLNYPVIELP
                     ATSIKERLEIILSHIDT"
     gene            complement(30525..31940)
                     /locus_tag="VAH76_000622"
     CDS             complement(30525..31940)
                     /locus_tag="VAH76_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO1385270.1"
                     /translation="MFKKILVANRGEIALRIVRACKEMGIAAVTIHSEVDRFALHVKR
                     ASHSHCLSKKPLDAYLNGRRIIECAKVAGCEAIHPGYGFLSENAEFAEDCNKAGIVFI
                     GPSAPVMATMGSKIAAREAMIKAGVPTIPGSKGNLETIDDALHCAETLGYPVMLKATF
                     GGGGRGIRLCHDASQIKQQFARVQSEAEKSFGDSHIYMEKYIANPRHIEVQILADHHG
                     TVLHLFERDCSIQRRHQKLVEIAPSPQLDEATRQVLLGYAVKAAKEVGYTNAGTVEFI
                     LDKNNQPYFMEMNTRLQVEHPITEQITGIDLVQEQIRIAAGERLTISQEQIKYNGFAI
                     EFRINAEDPQNDFLPSFGRITRYYAPGGPGVRTDSAIYTGYQIPPDYDSLCAKLTVWA
                     FDWPSVLRRARRALEEMRMFGVKTTIPYYLEMIKSEEFQKGVFNTGLVEAHPEWLKYS
                     NKSFPQHKAAVIAAAIALNKT"
     gene            complement(31933..32649)
                     /locus_tag="VAH76_000623"
     CDS             complement(31933..32649)
                     /locus_tag="VAH76_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEO1385271.1"
                     /translation="MYSSNYIYQHDDLELHGFLAHEEGIKQSRPAVIVAHDWSGRGEF
                     ACLKAKQLAEMGYIGFALDMYGQGQIGSNNDEKMALMQPLYSDRSLLRERLYAALEAI
                     KSISVVDNNRIAIIGFCFGGLCALDLARSGADIRGAVSFHGLLNKPENLASGRIKAKI
                     LALHGYDDPMAKPEQVHAFCQEMTEANVDWQMHMYGQVKHAFTNPQAHDEHLGLIYDE
                     VAARRSWQAMSQFLQEIFNV"
     gene            32931..33797
                     /locus_tag="VAH76_000624"
     CDS             32931..33797
                     /locus_tag="VAH76_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1385272.1"
                     /translation="MMTDSLIHIRDLLHAQFCYQMFITPIHLPIDKKYREFAKMACEF
                     IEGRRDEIIHREYPRHHVLHRFTPANKPNGKKILITHGWMSRAAYMVRLIRLLHKEGY
                     EVYAIDFPAHGEAKGIQLPWADAIAIIKETINQFGPFYGLVGHSFGGSMILNTLNLAG
                     QLPEWQLNHKPERAILIASPTQMRTPVNKIARRFKLSGQAYLQLRQLIRQQASVDPER
                     IRLSHFISQAPDTSFLCIHGELDATINPKESINFCKYYKNARLSLLPDADHVSVLMDE
                     RVEQIALDFLNP"
     gene            complement(33932..34786)
                     /gene="rpoH"
                     /locus_tag="VAH76_000625"
     CDS             complement(33932..34786)
                     /gene="rpoH"
                     /locus_tag="VAH76_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEO1385273.1"
                     /translation="MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHS
                     EGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRL
                     VSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
                     DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDT
                     GDQGREKLLFAMEQLDERSQDILQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMK
                     KIRQYMEG"
BASE COUNT        10824 a         7487 c         6274 g        10263 t
ORIGIN      
        1 ccaacccaag ctggaatcac aaaaagctca tgaactggaa tctattccac aacctctacg
       61 ttgccaaagg ttgtggagat aaagcaattt tcccctcatt gtcttctagc tcgacatgat
      121 cctcgcaata ggtgcgtccg ataattttac aaagattaat tttatccttc cttgataagt
      181 aagaagctac tgcttccacc atattttcaa atctgttttc gccagtttct tcatagggaa
      241 ggatattttc attttccatt ggagcattat cgtcaatttg ataattatcc acaatggcca
      301 tataaatgct gttaaataac gattttgctt ttttaatttt gccttgtaag gttctttctt
      361 ccgcacttct tggtgctgaa aataaagaaa gcgcccagcc ataccaacct ccaagtttag
      421 gttcttcttg cttaggctca ataaaagaaa aagctttata acatccaact accttattga
      481 ttaattcatc tttataaggc cgatattttt caggtaaatt gctattgatt aataaccact
      541 ccaggcgctg cagttttctg acaattgctt tcacttcttc gcaatattcc ccatctattt
      601 gcaataattt gattgtctct tcagataatg cttcaagttc ttgcagagga actgtccaat
      661 aaagctttac tccaggatcc tttaaatcac gatagtgcac aacgtcccct gaagatttcc
      721 tatgctcccg taaaggaccc gtactatgta aaaaaattga ctccattggc ccactacgtc
      781 tgggcactgt gggatgttca ccatattctg taaggcgttc aatttgctcc tttgataata
      841 cataagacag caatgataaa gcttgtgttt tacagttttg tgaaagagct aaactaggcc
      901 caaacaatcc ccaggacaaa cgcaactcaa aaggttttaa gcgagcttcc cgcccttgtt
      961 gttccgcttt aatattttca gtcgcataaa tcaatcttga aaattgttcg tgaggataaa
     1021 ttctggtttt ttcaggtggc ttccccttaa tgccttgcgt ttcgacaaac tcatttatcg
     1081 ctgtttcctg atcagttgcc atctgcaaac actttttttg taataactca aaatcgtctt
     1141 tggttaactc aaaggtaacc ccgcgcaaac catatccctg atcgagaaat ctgagctctt
     1201 catgccgcaa cattccatga ttgccaatca aatcaacatc cagccccatt tttattttca
     1261 attgatctaa ccaagaatta ttacgattgg ttgtcggtaa tccataaaat ccccaattat
     1321 caacaacctc cagcaattta ctggtttcat ccatttttga aagcaataga caactgtgcc
     1381 acagtggatt actccctacc tcctgatctg tagtacaaaa tgtaacagca tatttcatat
     1441 caccaccctc atcgatttgc atctaattat ttgaccttga ttaaatagac cttttgccta
     1501 aagccgattc tttcatttta ttgctgcttc gctaacattt ctgcattgct cattaactgc
     1561 attataaact ccgctcctca aaacgtttgc accttgccct aaaaaataat cggcttaggc
     1621 ataaagccta actaacaatt taaatctcta aaagattatt tttaaaatta caataaggca
     1681 tggcttcact ttcgtaaata gccaagatga gcgcaaccct tcatgtgttt aatataatcg
     1741 aggcaacaaa tgcgggctgg gccttgtggc ccagaaaaat cggataattg attagtaaaa
     1801 ttgaaatatc atctgttaaa tagctcaaag agccgagcaa tgatcttaac ccaagttatt
     1861 cattaatttg gcttgattca actgctcata agctcgcttt gcagggataa aattgggata
     1921 ttgcgtcacc agacgttgca agatcattct ggacaaaacc tcctccccat gattccattc
     1981 atttaatgca tcaaaataaa tcatatcagc gctttggcca tcaaacttgg gaacattatt
     2041 aaataatacg ggctcgacat aacttacttt accctgggtt tgtgcatcta atctttgcaa
     2101 tactctacgg gcctgtaaac ttccgttttc actcgcttgt tgtaatagct cacggccttt
     2161 ttcgggtagt cgttcgcctg ccgttccctc aatataataa gtacctaatt gatattgagc
     2221 ataagcattt tgcttttgtg ccaatttttg ataaatactc gctgaccttt tatgatcctt
     2281 ttcaacacca agaccataat gatacattct tgccaaagcc agcattgctt tttcattacc
     2341 tttatcagat gcatcctggt aataccgtaa agcatctgga aaatccaatt taacaccaac
     2401 accagtttcg gataataaac cgagggcata caaagcattc ccattaccta agcttgcagc
     2461 ctttttatac cagaccaaag cttgttgttc gtttcttggt tgccccaaac cataaaaata
     2521 cattccagcc aattgactca tcgcttcagg agcacctttc tctgatgctt ctttaaacaa
     2581 agataacgct ttggagtaat tgaggggcac gcccttgcca tactcataca ttaaagccaa
     2641 attataagcg cctagcatat ctccttttgc tgcagcattt tcataggctt ttagtgcttg
     2701 agcataatta tcatctaccg tttcataaat aaaacctaat gctacatcgg ctttagataa
     2761 atgtttcgct gctttttgat accagtattt ggccaaatta taatcaggtt ctcctgtttc
     2821 acctagttga taaaattggc ctaataagta ttgtgcctca ggattgcctt gttctgccga
     2881 agcagtatac caatgttgcg cgccagcaag atcaggcgcg gcacccaatc ctttttgtag
     2941 catataagcc aattttaatt gtgcatattg atcgccttta tcagctaagc ccttataaat
     3001 ttccttagct tgattcattt gttcaggaac gctgctttga gataaataat aatctgccaa
     3061 tactatcccc gcatgactat tccctaattt ataagattca attaagttgg gtaaaaagtc
     3121 ttcccctgat tgttgcttga gtactgccag attaaagtcg gcataggaaa attgggagtc
     3181 tgctgattgc ttaagcaaat ccaatccttt ttccttatcc tgtgccaccc cttttccttc
     3241 tgtgatgtaa gtacccaata taaactgact tacgggattt tgtccggctt gttgatacca
     3301 gtacatggcc ttggctggat cagcggttat tcctatacca cggtcataga gcattccgag
     3361 taaaagagcg gcattcgcat tacctgtttc agcttgctct tcggctactt taaatgcttg
     3421 ttcctgcttt tgcctgttat catccatcgc attataaaat gccaatggca acaatgcctc
     3481 tgccacttta ttcttcacag caccttggta taactttctt attaaggcaa ttttttgttt
     3541 tctgacatct acacttaaac cattgttgta gtctcttgcc aaatattctg ccaactcata
     3601 ttctgccggc ccataatcat tggcagctga aagataaagc atggccatag cttgctcatc
     3661 atttgcttta atatactctt ttccatcagg accaacgatc ccctggcgca atatccttgc
     3721 caatacgtat tgtgctcttt gattgccttt gaatgcggag tcagtcagcc aatttaatgc
     3781 ttgttggtaa tcattttcgt ctttaccgcg tttcagataa agtattccca aattatattc
     3841 agcacccaag tgcttttgtt ctgcggcatt ctcataaaaa ataatagccg aagcatcatc
     3901 ttgcattaaa ccaatcccat attgaaacaa ttgaccaatt tcaaattgag tttgagcatt
     3961 tccgagtatt gccctgaaat acaaataatt gaccacagac atataattga cagtccgttt
     4021 ccaatcccct gtccaaatgt ccatcaaatc aaaactgcca acttcgctat catcataatt
     4081 cgcgtcggca taaggaactg gcaagttgag cttttgcaca acaggactat tggctctctc
     4141 tagagcctct acgaatgggt taagtctgta agttggataa gcccattgat ttgattgaaa
     4201 ctccacttgt ttactgatta aagcatcata gtaattgctt ataggaatac tattaggctg
     4261 agtcaactca aattgtggtt tgaaaatatc ctgacgagag atggtttcaa gcttaggtgc
     4321 ttgattatag ctattatttc tcagggcaga cggattattc catgggctaa atataccatt
     4381 catctggtaa gccgtctctt ccaatttatc cgttttgtca ttacttagcc ataatgcggc
     4441 ttgggcaaga acagggggct ctgcattttt cttctcgttt tctgctgctt taagagtcca
     4501 ttgtttagac aattcttcat cagccgctac gccaatccct ttttgatata aattggctaa
     4561 ttctttttgg gcttgaacag aaccgttctg agctgctttt aataccaata aaaaacctgt
     4621 ttttggatca taaactggac ttttatcttg taaataaata tgggctaact caagagtagc
     4681 ttctgtactc tgttcctcgg ccttattaag ccattgaata gcctcatcgt attgattttg
     4741 cattaaagcc aatttaccca actcaagcat ggctggagta tattgttctg aggctgcttt
     4801 atttaataat tcgacgccct tggcagtatc cttggcaacc atttgtccgg cgacataaaa
     4861 tctgcccaat tctgttaaag cttttggatc tccattttct gctgctttat tcagccaaat
     4921 gatccctaat tttttattag ccgcatgctt actctctaag aaatactctg ctaaggtgta
     4981 ttgagcaata ggattaccat tttttgctgc atcaatataa taacgtcttg cgatatctgt
     5041 atttttcttg acgccaacac catataaata agctgccgcc atatacattt gtgcgctcac
     5101 atctcccacc gcagctgctt gcttaaacca acgggcagct tcttccggat ttttttcatg
     5161 gagcaaactg tatttggcca tgagcaagac agcaggtaaa tagccttttt ccgccgattt
     5221 ggtaaagtag cgcatcgcaa cacgattgtt ttttaactgc ccatatccat agagataaag
     5281 tcgccctaaa tagtattcgg caacagcatc ctgacctgat ttaatgatta ggggctcagc
     5341 tgcttcatta taattgccta atctataagc attgaacgca tcattggcaa aagcctgttg
     5401 gccagctgac gcgactaaaa cacacaccca aggtactagt gatttcatga agattcccct
     5461 cttttcatgg ccatttatac accgggtata atgccataat gcaccacttg actaactgcc
     5521 ccatttctcg tattgctgtc tactatccaa aaattacctt tattattcaa accaaatttg
     5581 acattaacat attcacatac tttgactgca tccgcacaat ccagtatttt tccataggac
     5641 tttttgccat cacccaaggt acagatgaac ttcacttcct tttcacctgt agccaaaaca
     5701 gcccatgagt tggctctgat cgaatgattt aaccagtaag aatacaaact ctcactgcca
     5761 tgcatctgat ataaactcac agtttgaggt aatttattgt aaataaaata catggattgt
     5821 agggccccct cttttccagg aagcagcgtc aaaactttta aagtttgttg atagccaact
     5881 gcagtacagc ctaagggaaa tttctcggcg tctttttttt cagttttttc atccgaattt
     5941 ttagcaattt tagattttgc tgcttgggct attactccac catatccgag gagaagaacc
     6001 aatactgagg cagtcattaa accattactc atcttgctca tattttattc tctctattta
     6061 tttaaaggac gcacaatgac tttagttccc ataaaattat tacgctcact ggacaactct
     6121 acaaaatttt cgtttaacca cttggcatcc tggataaaca tacgcacaca accgtggcta
     6181 gcccttaccc ccgggatatc atcagagcca tgtaaagcaa accctttatg aaaatacatg
     6241 caataaggca ttttggcgcc acctctgcct ataggaaaaa cacctgaggt acaacgaaca
     6301 ttttccttgc tgaatacgcg gaaaattcct gtcaaggtac gacagacttt attggaatca
     6361 gagcatttat cactcccaga agaaataggt ccccaccata ctaaattgcc ttgcgcatca
     6421 taggcgcccc aagctagctt ctcctgatcc acgataattt gcttttcatt ttcactgtca
     6481 attttcagtg ggaaaggaga gacatcaaaa atattgatgt tagctaaatt ctttggtaca
     6541 gcgatttctc tgcctgacca aaggtagtta taagtacgat tgactctttg cacaatgtct
     6601 ctttggacag gatcagggaa taatttttcc caactttgtc ccccggttac cttaatacat
     6661 tcatattgag gataagcaca taatgccgct ccataaaaat gcccggcgtt aagtgcagtc
     6721 accattgaca tcatgaacgt tgttaatagt atttttttca tggtgccgcc cccttatgcc
     6781 tgatttttat aattttatgc aaaatttgtg ctaaaaatga aatgccactc tttttatctt
     6841 ttttcggtta tttttaattt aagtttagac ccatacaaaa aaaattgaaa ttattgctat
     6901 atttaaatta gcattgactg ggttgaacac tcatgagtcc tttaaaaagt aaaattaaaa
     6961 caaagtcttt caacaaatta acaaaaactc aagacattac caacacgatt gaaagattat
     7021 ttgcaaactc tcaagacagc atcattgttt ttaatcaaca aggcaaaatt attgctatta
     7081 atgaaaaact gctgctttat ttaaaaaaag ccctgacaaa ctcattgaca aaccgatttg
     7141 gaaactattt aagttaaata gcttcgctat taagaggcaa tttattcaag caaagaggtg
     7201 ttttctttta gccaatgaag ggatagccca gcaattcact tggcttgaaa gcaaagcaca
     7261 aaaaccagta cttgcatacc atatcctgtt caataaaaca gaaatagaag acacatcaat
     7321 catttttacc aaacttaccg atattctaaa atcgaaaacc atagaatgga ttttatggtc
     7381 tttaactaaa ataagtaacc accaggaaat caatgaaatt attgatcaaa ttctacaatt
     7441 aaccagtgat gtttttgcag cggattatac ttctgtatct ttcataaaca atcaacaaat
     7501 tgcaaggaca atcagctact ataaatttgg aaagaaagaa gagaacatta gtttttcttt
     7561 aatcaataca ccctgcgcta aagtaatcca gaaaaaagct atctgttttt aatgacgttc
     7621 aaaagcaatt tccaaacgat aaattattga aaaggatgaa catcaatact tatctcgcag
     7681 gcccaattac aaatccccag gatgaggtga ttggcataat gactatcctg tgtaaacgca
     7741 aagtcgaatt tgacacactg aataatacct tgtttggttt atttttaagc cgaatcaatt
     7801 cggagattga gagactgata aaccttagaa aattagagtt tctggcgagt ataccccagc
     7861 aaaaccctaa tccaatcata aggattcttc tcaatgggga tgtcgtattt actaacgatg
     7921 aagggaaaat cattttaaac tactggataa aattatttaa gggacttcca gtagagttac
     7981 taaaagaagc acaaagaatt cagaaatcca atcaaagcac aagaattgaa atggaaattg
     8041 aggataaaat ctacttattc acacttgtct ggattgacga atttaaacag atcaatattt
     8101 acgggactga tataacccag ctcaagaata cgcaaaacgc tatgttaaat ctaacccgca
     8161 gagatacttt aactcagatc gcaaatcgac aatattttga agaaaaacta attgaaaaaa
     8221 tatatgagca tcatctgaaa gaaaaaagcc tggcattatt attaatcgat ttagataact
     8281 ttaaaattgt taatgataca ttagggcatc caacaggaga caaactactg atggctgtta
     8341 ccaatagaat gacccgatgc ctaagagcaa atgattttat tgcccgcctt ggaggagatg
     8401 agttcatcgt ccttcttgat caatgcaata ccgataccgc agttaaggtt gcagaaaaga
     8461 ttattaacgt gctcggcaaa cctttccaat ttgatgaata tcatatgaca atcacagcca
     8521 gtattggcat tgccatctat cctgaaacaa gtgaaatagc cagcgactta ctaaagtatg
     8581 ccgatattgc aatgtaccaa tccaaaaaaa caggtaaaaa caagtgcact gtgttttcaa
     8641 aaaacttaca ctataaggaa aatcaaagaa atgaaattct caaaaaagac atagaattag
     8701 cagcattaaa aagagagctc tatatagact accaacccca aattgactta tccgataaca
     8761 caatagttgg aattgaagct ttattacgat ggtcacaccc tactctgggt ttaatattac
     8821 ctactgagtt tattcctata gcagagcaaa cagggtgtat acataccatc agtcaatggt
     8881 tcatagaaca gacattactt gattactccc agtttcagca attaagttca aacatcaaat
     8941 tgtcgataaa tgtttccttg agtcaattaa atgacacgcg atttactaaa atattgtgtg
     9001 atagtttatt gcaaaataat ttaagcaaga gcaagatcat cttagatata tcagaacgta
     9061 tgattgcccc acactttatt caaatatcaa aaaaattaaa acaaatacat catattggaa
     9121 ttaatataag cttggataat ttttgctgcc cacaaatttc cctccctgaa ttgttgtcat
     9181 taccacttga ttacctcaaa gtagatcaac gattattaac aggcatagag ggaaaaatca
     9241 aattacgtaa attattatca ggaataatca atttaactaa agatcttaat attaaaatca
     9301 ttcaaaaggg tattgaaact gcagaacagc atgaagtgat taaatcttta ggatgccaat
     9361 acgcacaagg atatttttac tgcaaacctg ttgccttaaa tgatttactg ccacttataa
     9421 aaaaaactat ccactaagta attaataatt caacctaatt ttatggcatc aaacgcaata
     9481 ttcttataag attctaaatg ttttgaacca ttacttagac agtttttcgt cataattaat
     9541 tgatataaga atcatacatt gcaatggaag ttagcttatg cctttttgtt tttccaacca
     9601 accaaatatt ttaaataaca aacttattct aaaaatagtt gtgcttattc tcttctttag
     9661 taattcgagc tttgcgaaaa caatcaatta tccatggaca gtctccgcaa gcttggggta
     9721 tgtcaattat caatacgtac acgaaggcga ccatattacc gcaacaggtc ggtttgcaat
     9781 aggaagagat ttgttgatga aaggcccctt tttatttgga cttgaattag gtttacaaag
     9841 tgggaatcaa atgcgactgc aaatgacgga agaagaggtc gatgccctcg gaggactttt
     9901 ggtacaaacc accttgaaac ctatggttga tgtattagct acaatccgta ttacccctga
     9961 atttaccgcc cctttgtttc tacaactgaa agcaggaact gcctacaggc aatggcaaat
    10021 ggatagagac acgatcaatg acatctctca acttgcaggc gaaataatgg ctggatgtgg
    10081 ttatgcaatt acaccaaatg ccagtattac cctatcgtat caaggcctat ttggagacaa
    10141 accaaatttc actattgatc tggaaagtgg aattgggcat gtacaaaaga ttcctgttca
    10201 acacgccatt ttgttaggta ttgttttaat gatttaacta atttttctaa tagatctctt
    10261 gcataacctg cttattttgt tttaggacaa ggtacaaacg ttttgagaag tcagatttac
    10321 aagcagtaaa taagtaccgc agaaacgctt gtaacgcagc agtaaaataa aagagacaag
    10381 tgagcatata gtgggaaata aagaatgata catcgaaata aagtccttaa tagcatttta
    10441 ttaatcttat tcacatcaca gatagcattt gccaaaagca atccggcctc gcaagcctat
    10501 gtggacggca aggtatctga attaaaaaat gaattaacca acaaaatcaa cagtatcccc
    10561 tctggaccgc aaggacccaa aggagataaa ggcgaggctg ggcctaaagg agaccgaggg
    10621 gaggcaggac ctcagggatt acctggactg aaaggagacc gaggagaggc aggacctcag
    10681 ggattacccg ggccgaaagg agaccgaggg gaggcaggac ctcagggatt acccgggccg
    10741 aaaggagacc aaggggaagc aggacctcag ggattacccg ggccgaaagg agacagaggg
    10801 gaggcagggc cgcagggatt acccggccct aaaggagacc gaggggaggc aggacctcag
    10861 ggattacccg gtccgaaagg agaccgaggg gaggcaggac ctcagggatt acccgggccg
    10921 aaaggagacc gaggggaggc aggacctcag ggattacccg ggccgaaagg agacagaggg
    10981 gaagcaggac ctcagggatt accaggccct aaaggagaca aaggagaaac aggagcagta
    11041 ggtccgcaag gtatgccagg acctaaaggc gaagcaggag atgacggcca aggtgtgcct
    11101 gcaggtggtg aaacgggtca agtccttgcc aaatcaaatg acctcgattt caataccgta
    11161 tgggttgacc cggcaaattc cggtattagg cggcaattag gcgataaagc tcttggcggt
    11221 acagtaattt atgttaatgc gctaggtacc catgggcttg tagtagcaaa ttcagatcaa
    11281 gttaattcaa acacatggtg ggatgctcaa gattccataa ccaatcctgc ccactttgac
    11341 aatgaaggta aattatattc tgactggagg ctccctagcc gttttgaatt gaatttaatt
    11401 tatatgatgc gtgatgagct gggaaatttt ttggcaggta attactggag ttcgattgaa
    11461 aaatcatcgg caaacagctg ggtatttaat tctaaaacag gggaaattaa agacattgca
    11521 aaaagtaaaa cggctgctgt acgtgctgta agagcctttt aagtatggca aaacttcaat
    11581 tttgatcaag ggtggaaatg ttcataaatt cgctttgcca actgctcgtt gattccctgg
    11641 actttacaaa tttcttccag tggcgcttta gccaactctc gcaacccgcc aaaacgctgc
    11701 agcaacgctt gacgtcgttt cgtgccaaca ccctctatac tttcaagagt ggattcaaca
    11761 cgtgtctttt gtcttttctt ccgatgggca gtaatagcaa aacgatgcgc ttcatcacga
    11821 atatgttgta aaagatgaag cgctttggag tcctctggca aaacaaattc tctgttttca
    11881 ttaaccagta tcaatttctc ccatcccgcc tttcgtgaag gccccttgga tacacctaat
    11941 agagtaatat cttccacacc cagagaggtt ataactcgtt ttacaataga aacctgacct
    12001 ttacctccat caataatcaa aacatctggc aataattgag catcaatcag tcttttaaat
    12061 cggcgagtaa ctgcctgttc cattgctgcg taatcatccc ctggagtaat atcctcaatg
    12121 ttaaatcgcc tgtattcact ggggcgaggg ccttctgtat caaatacaac acaagatgcg
    12181 acagttgatt caccttgagt atggctaata tcaaaacact ccattcgctc tatattttta
    12241 tcaagcgcaa gcaattgctt taatgcctga tatcttgatt ttatcgtaga gtgtttgctg
    12301 acatattctg ctactgaaat tcgtaaattg ttcactgcaa aatccatcca cctcgatttg
    12361 actcctcttg gattgacttg aattttacag gatttgcctc gtcgctggga caaaatatat
    12421 tctaatgaac gactctctgt tatcgattga ttagtaatta ttaaatccgg aattctttct
    12481 gatgtatcca aataataaaa accaacaaag gcttcaaaag tctgctgcca aagtgaattg
    12541 gcatccaatt cttcatctaa tactgcataa ggcactgtag ggaaaaagct ttgactattc
    12601 agtacttgtc cttcacgaat agtcacacac tgaatacatg cgaaaccagg acgtacttct
    12661 attgcgatca catcagcatc accacgtaac tggacaactc cttgttgttc ttgaattaag
    12721 cgcaaatttt ttatctgatc acgcaaaaca gcagcttctt cgaaatttaa ttgacttacc
    12781 gcttgtttca ttcgttcggc aagttcatca agaattattt ggcacttccc ctgtaaaaat
    12841 cttatggcat cctctacaga caatttgtag ttttcaggag aaatataatg aacgcaaggc
    12901 gcagtacaac gttttatttg atattgcaaa caagggcgag aacgagcttt aaaatagctg
    12961 tctctgcaat tgcgaatttt aaatattttt tgaatggtga ctatagtttc tcttacagca
    13021 gctacaccag gataggggcc aaaaaaatta ccggagggag gcttcttttt acttcggtat
    13081 aactcaattc ttggaaagtc tggatgattt gatagatgaa tataaggata ggatttgtca
    13141 tcgcgcaaca aaacattata ttttggcctt aacgctttaa ttaaattact ctctaataat
    13201 aaggcctctg tctccgatct tgtcacagaa atttctatgg atttgatttg gcttaccagt
    13261 gctcgtgtct taactcctgt attttgttta ctaaaatagc tattgactct tttttttaga
    13321 ttggctgctt ttcccacata tagaacggtg ccttcctcgt ctaacatgcg gtatataccc
    13381 ggttcacctg ggagtttcgt taaaaataaa gcgagttcag cggaaagctg taagtcattc
    13441 atgaaattaa catctaatca tcttgtgaca aatcattggg gtgttcaata atgcggtgct
    13501 tcatagccaa ataagtaagc tccacatcat ttttaatccc caatttttca aacatcctat
    13561 atcgataacc attgatggtt ttactactta aaaacaatct atcagcaatg tcttgtacat
    13621 tcatgccgct cgtaatcatc aacatgactt gcatttctct ttcagacaat aaatcaaagg
    13681 gggaatcctg aggctcctga agactattaa ttgccatttt ttgtgcaatc tcggcactaa
    13741 gatatttctc acctttagcc actttacgaa ttgcagcagc catttcttca gcccctgatt
    13801 ctttagtaag gtatcccata gcacctaatt gcaatactcg agtagggaga ggatctgagc
    13861 aaatagctgt aacggctatg actttaatat ttgggtttgt tttttttaag cgacgcgtca
    13921 cttcccaccc atctatccca ggcattttca tgtctaataa tacgacatca ggggagtggg
    13981 ttttcacata agctaaagcc tgctcaccac tttcagcatc tgcaaccaca tcaacatccg
    14041 acatatcttc aagcaatcgt cgaatgccca ttctaaccaa tgcatggtca tcaacaatta
    14101 atactttaat caaataatgc tccttgacca atcagtcaat gaacaaactg tgttggtcga
    14161 tgttaacaag actcgataat cattacaata catagtcatt ctaatccaca agtccagttg
    14221 tcttacaaaa taacaaacta acaagccctt atatgaatat ttggattatc cggatccaca
    14281 tcaaaatacg gtggaaattc acctaactct cgcgcacgat ccatgaattt acccatgtca
    14341 gaaagcacga agtcgtataa attttgataa ctgtcaataa cgaaataaac aggttgtagc
    14401 tggtcaatcc gataaggcat tcgaaaagcc actactggat caaataaaac tcgcacagga
    14461 atatcacttt ccacacaata taccgtttca ctaatagaag ataaaattcc cccgccgtat
    14521 gctctaagcc ctttaggcgt tttaatcaat ccaaactcta cagtaaacca aaacattctt
    14581 tgcaataaag gccaatcctg ttcaggaaaa gttaatacct tacatgcgta atcatggaca
    14641 aattcagcat agactctatc ggttaacata ggacaatgac caaaaagctc atgaaaaata
    14701 tcaggctctt gaacataatc caattcttct tcacttcgaa taaaagtcgc cgcaggaaaa
    14761 tatttttctg ctaataattc aaaaaattcc ctggctgaaa ttaaagcagc aactggcgct
    14821 acttgccatc ccgttttggc ctttaatcgc tcacttactt ctggaagttg tggaatagtc
    14881 gaggggttaa gtcctaaagt ctgtaatcca gacagaaatt catcacaagc tcttcctggc
    14941 aataacttga gttgtctctc aaataaaata ttccaaattc tattttcttg tgccgaataa
    15001 tcgactaaac cctgagcatc agggacatgt gcgacatacc ggctactaaa ctccataatc
    15061 tctccttcac tcatctcatt caaaaatcaa taaccaggga taataatatt tatttgtatt
    15121 tggggtcagc ctattaaagt caacgagaat ttttacctaa aagttgcttt attttgagta
    15181 tacatcctag ccaataccct gttttagatt ggcattatta tgatgatcac taaactctct
    15241 aacttgtttt agtaattacc taacagtaaa ctaaatgtca acatgattac cattatcttt
    15301 ttgagatacc ctctaacaat ggaaagcaat gagtaacttt ccgatatggc aaaaattacc
    15361 aactagtgta tgtggggtaa ctggggcatc ataaaacgat aacttaatct gttaaccctt
    15421 atatattaat tattacttat tttacagatt ctgctataga atttattaca gaaattaccc
    15481 attggaatac acaaaatgac aatagctatc ttgtctaccg gagatgaaat aatacatggc
    15541 gatactctaa ataccaatgc gcattacatt gctcatgccc tttgctctga aggcttacct
    15601 cttggattac aagtggcctg tagcgataag gaaaatgaga ttatagatag cctaaacttc
    15661 ctgacggaac accatgatat tctaatttta attggtgggc ttggcccaac gaaggatgat
    15721 attactcgct ttgctttggc taaatttacc aaagaacctc ttattcagca tgctgatgct
    15781 ctggatcata tagaaaaaaa agcaagcata gccaaaatat cactgaatca aggcaatttg
    15841 cagcaatgcc tctttccagc caatgcacga cttttcccta acccttatgg cacagccatg
    15901 ggatgttctt attcatggaa aggtaaagtt tttattttat tgcctgggcc tcctcgtgaa
    15961 tgccttccaa tgtttaatca ggatgtactg ccgttattat tgcaaacatc caggcatagc
    16021 aataaacaaa ttctgcgctg gcgaattttt ggcttggctg aaagtgaaat agctcaaatc
    16081 cttgaagaag ctctggaagg tttagattgt cagacaggat accgattgga aacgccttat
    16141 ctggagttta aggttcgctg caaaaaggaa ttggttgaaa aaataacatc aataatagat
    16201 cccattttag ccccgcacat tatatcatca cttgaaacca aagcatctga tcaattgaga
    16261 agcctgatcg taaagaataa tgaaccaata actattattg atgatgttag tggaggatta
    16321 ttgcaatccc tattggtaag accagaaaac taccatttat taaactttca taatcttaac
    16381 aggaccaaat tatattttca ttgcagtgga ttagaaaatt attggtcaca acaagcaccc
    16441 caaggcacta ccaaattaat cattcactac agcaatcaaa tgcaagaagg tcgtgaaaca
    16501 catcagattc cttatcgaag tgctatggtt gtgcattatg ccgctgaatg gctaagcttc
    16561 agactctttc atctcatcaa tcaattgcat taaagcgtag caaagttctt gtgttccctt
    16621 tgtctctata gcggaaatag aaaatacttt gtctttccac tccaagcctt ttattatgga
    16681 ttgaattttt tcttctcttt ccttttcatc aggtaacata tcgattttat ttaaaaccaa
    16741 ccatctgggt ttgttgagca gatcgggatt atattgagtc aactcattaa gaattgcttt
    16801 cgcatcagcg acaggatcac tcccatccaa aggcgcaata tcaataacat gcagcaagac
    16861 acaggtacgt gaaagatgtt ttaaaaaccg atgccccaat cctgctcctt gagctgcgcc
    16921 ttctataaga cctgggatat ctgccatgac aaaactctta tagggtgaaa cacggaccac
    16981 ccctaaacct ggatgtaagg ttgtaaatgg atagtcagcc actttggctt ttgaactgga
    17041 gacggctctt attaaagtag actttcctgc attgggaagc cctaacagac ctacgtctgc
    17101 caaaactcga agctctaaac gcaaatttct ggattcaccc gggcttccgg gtgttgtttg
    17161 tctaggtgat cgattcacgc tgctcttata gcgagtgtta cccaaaccat gaaacccacc
    17221 ctgagcaatc agcacgggaa cacctggttg actgatatca gctaacaatt cacctgtatc
    17281 ggcatcataa accatagtgc cgactggcac tttgatggtt aaatcctctc ctttcttccc
    17341 agtacaattt ccacccatcc ctgattgtcc attctctgct ttgtattgac gagtataacg
    17401 aaaatcaatc aaggtattta aatcgctgct cgcctcaaaa taaatactgc ctccatcacc
    17461 cccatcaccc ccgtcagggc caccacgagg aataaatttt tctctcctga agcttaagca
    17521 accattccct ccttttccgg cttcaacttt aataagtgct tcatcaacga atttcatgcc
    17581 taaccttaga ctaaaatagg acttacgcaa aattccaagc tcgaagcaaa aaatattttc
    17641 aatgaatgcg tttagataag ctaagtgact gaattgaaaa tattttttag caaagagatt
    17701 gggattttgc ctaagtctgt aaaaaaaaac cccgtttccg gggttaaaca aaacgaatta
    17761 tgatgccatt gggtaatagc attgtttcag cttaactcgc agcttcttct cgttgctccg
    17821 gtaatatagt gacatactta cgattttttt cacccttggt agtaaactgt actaacccct
    17881 ctaccaacgc atacaaagta tgatctcgac cacaaccaac accaggtcca ggatgaaaac
    17941 gtgtgccacg ctgccttaca atgatttcac cggcatttac aaattgacca ccaaaacgtt
    18001 tcacaccgag gtactttgga ttcgagtcgc ggccattacg agtactacca cctgctttct
    18061 tatgagccat tgctatctcc tcttatttac caatcgcagt gattttaact tgcgaataat
    18121 attgtctgtg ccccatttgt ttcatatggt gcttacgtcg acgaaattta ataatcttaa
    18181 cttttttgtg acgaccatga tcaagcactt cggcctttac agttgcttta gcaacaaaag
    18241 gtgtgccaca agtgacctta tcaccatcca ctagcattaa tacttccgaa aatttaattt
    18301 cattgccaac attttcaggc agcatttcaa tttttaaaac gtcaccttct ttcacggtat
    18361 attgcttacc gccagtttta attaccgcat acataactat tctccaattc gcctgattgg
    18421 ctatcacaat tccaacaaag tgccatattc tatgaaatta tgactattac ttcaagttca
    18481 ttttaaaata ttgtatactt caccaccctt taccgattga cttaatgttt ttaaggcttt
    18541 cgagtaaatt ttaatctggt cgcggattaa ttaggaacta tagtcacatt taagatagtg
    18601 cctagaggga acccttaaat ttttggagtt tttataatgt caacgattca attagaagca
    18661 caatcaagaa cggatatggg gaaaggtgcg agccgccgcc tacgtcgtct tgaaaataaa
    18721 gttcctgctg tcatatatgg tggaagcaaa aagcctatgg caatccattt tagccataat
    18781 aaagtgatta aagcattgga aacagaaagc atttattcca gcgtttttga tatcacagta
    18841 gatggcaaag tagagcacgt tattttgaaa gccttgcaac gccatcccta caaaccaatc
    18901 gtgttacata tggatttaca acgcgtttcc agtaaagaca ttctggttaa gctagtacct
    18961 gtccacttca taaatgaaga acaatctcca ggaattaaag ctggcggtat tgttcaacat
    19021 accatgactc aagttgaaat acgttgccag gcaaaagatc tgcctgaatt tattgaagta
    19081 gatatgtcca aggtaggtat ggatgatgtg gttcacttat cggatttaaa acttccaaaa
    19141 ggcgttcagc tgacagtgga tgtaaccgat ggtagccatg atgctcctgt ggtgagtata
    19201 catgccgcta aagtaagttc aaccgaactg gaagaaacac ctgaagttcc agcatcggct
    19261 gtaccgacta cagatcaagg ggaaagtgct gaataagcac ttccctgtta tatatttatg
    19321 caaatattct gagcttaacc ctgagtattt gcataaagtc tagtgctaga attgtcaaca
    19381 aaaatccgct taattcaaat tttatagaaa atctatggcc attaaactaa ttgtcggttt
    19441 acgcaatccc ggatcggctt atgaacaaac cagacacaac gcaggtgcgt ggttagtgac
    19501 cgctttggcc caaagacata attcgcattt taagattgac aagaaaatgc aagcggaatt
    19561 aactgaaatt gatattaaca accacccatg tcgactagtg cttcctttgt cttttatgaa
    19621 tcatagtggt caaacaacaa gaataatcag ccaattctat aaaattgaac ccggtgaaat
    19681 attaattgtt catgacgagt tggatttacc tgttggacga atcaaattaa aaacaggtgg
    19741 tggccatggt gggcataatg gtttgagaga cataactgct caattgggaa caggtgtgtt
    19801 tcatcgatta cgtataggga taggtcatcc tggacataaa gatctagtcc atcaatacgt
    19861 actcagcagg ccttctatgc acgacaggca acaaatttat gatgcaatag acagaggtat
    19921 agcgataata cctatagtat tgtcagggga tatggcccga gcaatgaatc aagtaaacgc
    19981 ttaacataat gaggtatgac tcatgggatt taaatgtgga atagtaggat tgcctaatgt
    20041 agggaaatcg actcttttta atgcattgac taaggctggg atagaggcgg ccaattaccc
    20101 attttgtacc attgagccca atgtaggtat cgtcaccgtt cctgattcaa gattggataa
    20161 tttaagcgac atagtcaagc ctcagcaggt gattcatgcc accatgcaat ttgttgacat
    20221 tgcgggattg gtaaaaggcg cttccagtgg agaaggatta ggaaatcaat ttttggccaa
    20281 cattagagaa acagacgcta ttgctcacgt cgttcgctgc tttgacaact cggacgttgt
    20341 tcatgtggag gggcgagttg accccttaag tgacatagaa gtgattaata cagaactagc
    20401 attagcggat atggagacat tggaaaaatc gctattaaaa gtagggaaaa acagtaaaag
    20461 tggaaataaa gaagccattt ttgaattaaa aactctggaa aaaattaaag cacatctgga
    20521 taccggatat cctgtaagaa cacttgaact tacccctgaa gaagaacagg taagcaaacg
    20581 cctttttcta ttaacggcca aaccggtact ttacatagcc aatgtcgatg ataacggcta
    20641 tgagaataac cctctcctgg ataaagttaa ggctctggct tcccaggaaa atgccagcat
    20701 agtagccctt tgtgcagcaa cagaagccga gctggttgag ctggatgaag aagacagaca
    20761 agagtttatg gtagatttgg gacttagcga gcctggatta aataaagtga tccgcgccgg
    20821 gtatgaattg ctgggattgc aaacctattt caccgcagga gtgaaagaag taagagcctg
    20881 gacaatacgc aagggtgcca cagcaccgca agctgccggg gtcattcata ccgactttga
    20941 gaaaggattc atccgcgcgg aagtgatttc ttacgaagat tttattcgct ttggcggaga
    21001 acaaggcgcc aaagaagctg gaaaattgcg gcttgaaggc aaggaatata ttgtctgtga
    21061 cggagatgtc atgcattttc gctttaatgt ttaataatta gtattctgca gctggtgatt
    21121 tttcaagaat tgaaccaagg aacctcgatc ctctattgct tcatcattgc aagtaaccag
    21181 agcatcgacc cagacattga agcaagtgga agtaagccat caggcatgtg tcttactact
    21241 cttataccac actgactgtt attgtatttc ctgcagttac acccgacatc cagctagcaa
    21301 gagctaagct atttggcata aaccgaatag taaaccaacc tcaacgtgga cagtgtggtt
    21361 gttttttaat ctataattga attaataaac gaaattagcc ccattgatta ccggccaatg
    21421 cgattgagga aggtgaatgt cattactaaa gaaaacaaga gagcaggatt actctatcct
    21481 ggttaccact caagaccagc aaagaaagcg ccatttactt aatgttattt ccatagaact
    21541 tttaattttg ggtcttttgc tgatcataac aaacctctgc ctgctaagag cctggcttat
    21601 aatcagtatg ttaatcgtgg gcattttcat cttgctcgga aatttaatct taatcaaaaa
    21661 gaacttcaat ctggccttga gtgggcacat tttgaatatt cttgtgttgt ctatcatcat
    21721 tggaggcaat atcatcgttg gaaaaactac catgtcttac ctgggatggt tctatgtctc
    21781 tccaatcgtt gcttttgcta cacttggatt agccgggtta gtcgtctatg gtgtgctggc
    21841 gattggggca atgtttcttt ttgtattcga attctacatc cccatctatt ccatttctca
    21901 ggaatatgta ttttttctgg ataatactaa ccacctgttt atttttgccc ttattttcac
    21961 tgttctttat cattttctgg ttcaaaactc acaatatgaa ttgctgttaa aagagcaaaa
    22021 ttatctgctc attgccgata aagaaaaatt ccattacctt tcacatcacg attcattaac
    22081 caacttaccc aaccgcccct actttcatgc tcatcttcag accctattag agaatactga
    22141 cactaaaaag gaagccatta ctctttattt tatggatttg gataaattca agcccatcaa
    22201 tgacgaatac ggtcatgagt tcggtgatca gcttttgctt ttaaccagca agcgtttgca
    22261 atcctgcttt aggaaaaatg attttatagc ccgactggga ggagatgaat ttaccgcgat
    22321 tatcgttcat aaaccccatg atcaaattgc tgaggctcta actcttagaa ttgaacagga
    22381 atttgaacaa ccttttgtca taaatggcca gaccattcat tgcaccataa gcgttgggat
    22441 agcaaattac ccgcgcaatg caaaaaatgc ggaagatttg ttaaaatggg ctgataaagc
    22501 gatgtatgac aataaaagaa aaaaatatca aacgcttaaa caaacctctt aaccaacatt
    22561 cctaatttaa tcagtatgat tgggccttca ttttataaag cacaggatgc agcaatctga
    22621 cattcacttg acaattaact tttacaactt tagcattcat tgattaaata tcagagggta
    22681 aatgaatgac tattgaccgt atacgagcgc tggttagtga agattttaac gcggttaata
    22741 atttaattat tgataaaatt caatctcaag ttggtttaat tcaggacttg tcacaacata
    22801 tagtcgaaag tggcggcaaa cgtttacggc ctttagttgt cctgttagca agcaatgctt
    22861 gtggatacaa aggaaaagac catatttctc ttgccgctat ggttgagttt ttccatactg
    22921 ccactttgtt gcatgatgat gtgattgatg aatccacttt aagaagaggc cgacaaactg
    22981 caaatagcat ttggggcagt aaagccagtg ttttagttgg tgattatctt tttacccaat
    23041 ccgtaaatct aatggtagag gttggcatta aagaaattct caatctactg gccaatactt
    23101 cccatcaaat tacctgtggt gaagtcaaac aacttgccaa tcgccacaat cccgctttaa
    23161 cccttgatga ctatttcgac gtcatccggg ccaaaacagc tcttttattt gctgcggctg
    23221 cctgcatagg ccctatactc acaaatgcac ctattgagat tcgagatagt ttatataatt
    23281 acggtttaca cttgggaaat gcctttcaat tgattgatga tgccttggat tattgctcgg
    23341 atgctgccac tttaggtaaa aacattggcg acgatttagc tgatggaaaa gcgactttac
    23401 cactaataca tgcattacaa aatggtaatg cgactcaaca acagcaaata agagaaagtt
    23461 taatgaaagg cagtctaagc catctgcctg atattttaat tgctattgag caaaccgaag
    23521 ccataccgtt tacgaaacga attgctgccc aagaagtaga ctcagcacta tctgccctgg
    23581 gaatactgcc tgactcagtt tataaagaag ctttagtcga tttggccaaa tttgccttgg
    23641 aaagaaatta ttaagctgat aattgcatcg attctaataa ttacacaacc gattgataat
    23701 agtctggtgg agtgcggcat ttaaaacagg cagttttgca attggctcct ccctgacact
    23761 tccttattgc tcctttccta acccataagt ccagcatagg ttgcaatgcg gtcagatcca
    23821 aatgaaactc cctactcaac tgttgagtgc ttactacccc ttcccggtag ataaaatcac
    23881 gtatttgcag cagcattttg cctccccata ccataatggc tataacgaaa tacagctaaa
    23941 acaaaaagca ggctcagtga catagccaaa atccagacca tactttgttg aggatgatcc
    24001 agaaatgtag cgccttgata aaaaacaaca gaagtggcgt aagccacgac aaatgaccaa
    24061 acaatggaag tccacataaa tcgcttgttt gcctcctggc gaatcactgc catagtcgaa
    24121 acacaaggga tataaagcaa aacaaaaagc aaataagcgt aggctcctac agctccatca
    24181 aaacgtcgtg acataatgcc atagacagat tgagataact cactatcagc tgcactggca
    24241 gaaacagggt tccacaatgc agagcctaat tcagacaaat tggcgggaat agacccaaaa
    24301 gcagctttga tgccccccca aaaatcaaag tgggcagcag ctatttctcc cacatgacct
    24361 acttgggcat aaagtgagtt taaggtgcca accactacct ctttagccag catgccagtt
    24421 aataaaccga cggtcgcggg ccagttgtct tgatgaattc ccattggagc aaataatgga
    24481 gtaatccact gacctattat tgacaataaa gaatccgtat ttgcttctcc ggagctaatc
    24541 cctcctcccc aagtaatcgc atttaatcct cccagaatca cacaaatggg aataatcagc
    24601 ttacccgcac gataaacaaa aaatcgtaat cgcatggagg tttctctcag caatcttctg
    24661 agtgagggtc tatgataagc gggtaactcc aaaatcaaag gagaagcatg tcccttcaac
    24721 gtagttttgc gtaaaatata tccggtaaat acggccatta aaatgccaat caaatacaac
    24781 gaaaaaacaa tattatgccc gccagagggg aaaaaagccg cgacaaatac cgcataaata
    24841 gccagtcggg cactacaaga cataaatgga ctcatcatca cagttaataa tcgatcgcgc
    24901 tccgaatcca gggtgcgtgc tgccataatg gcagggacat tacatccaaa acccacaatc
    24961 ataggtacaa atgattttcc tggtaatccc atggctcgca tcgctttatc cacgacaaaa
    25021 gcagctcttg ccatatagcc agaagtttcc aataaggaca aaaagaaaaa catcgcggca
    25081 atcacaggaa taaaagtaat agtggtatta attcctttgc ctaccccatt agctacaagc
    25141 gcgattaccc aatttggtgc atggagctgt tgcaacaacc aaccactacc ctgaacaaag
    25201 atagtctctg tactaatgtc aaaaaaatcc tggaatgctc ccccaatatt aatagcaaac
    25261 aaaaacatta aatacatcat cgcaaaaaat atgggcaaag ctaaaaagcg atgcaaaaca
    25321 agtttatcca atttcgcagt gaagtgttcg ctggcatcac tatgcttttt ttgaaccaac
    25381 gtaacaattt catggatttt ttgatagcga gcgtctgcca gtaacacatc aagattttgc
    25441 tccgtttctt gaagctttat aagaagactc tcggtaaaag ctttctctcc tatcaaagta
    25501 tccccttcgg caagcctacg ggagaaatag taggcaaagg aatttttata cccttttgag
    25561 atcaactgat tttccaggtc atccagaact tgttgtgcaa caactgataa ggaaagtttt
    25621 agaggtttta tcttttggga gcaatgaagc aatgattgct gaagtgccgg aataccaatg
    25681 tttttatggg cctgaatggg aataacagaa catcctaaca agctctcgag tttctctgta
    25741 tcaatagaaa taccccgatg ctcggcaata tccatcatgt taagtgctac aacaacgggt
    25801 ttacctaact caaaaagttg gctggttaaa tacaaatgtc tttcaaggtg acaagcatct
    25861 ataacattaa tgatgcaatc gtattccaaa tcaataactg actgagcagc aatttgctca
    25921 tcctggctta ttccttcagc attcgcaacc agagaataga cacctggcaa atcagtgatt
    25981 tcaattaaat gttctccaag caaaaattca cctgtttttt tctcaacagt tactccgggc
    26041 cagttgccta cgcgttgatt cgcattggtc aaggcattga atagggttgt cttaccacaa
    26101 ttgggatttc ctattaatag ggcatgagtc atagatgctc caacactaat tgactcgctt
    26161 catctttacg caaagttaaa aatgttcccc ttacttcaat ttgtaccgga caccctaatg
    26221 gagccatcct cactacagaa aactcaattc cacaagtaat acccagggat aaaagcctgc
    26281 gtcgatactg catatcagtc gcaccaaaac tgaccaatcg tactttatcc ccttgtttta
    26341 attcactaat ctgcataata tataaacaat acggtgattc atttcattaa ttttaacaca
    26401 aacgatatcg agaatcattc tcaattaaga aattttatac cacattacag catttccttt
    26461 accaaacgcc gaggatgacg actcagatag tctaccgatt gcatctcaat tagacggctc
    26521 aacgttcttt taaacgtttc cttcatttca cctttaacgt acaattcttc tgcaggaaca
    26581 tccgctgaga taatgagctt aatgccccta tcatacataa catctataaa gtgaataaac
    26641 ataatcgctt gtgctgtatg actttcagta agctgataaa catcactcag gaagacatta
    26701 tcaaagcgat cagcaatttc taaataatcc aattggctac gaggaaaatt acaaatcaca
    26761 tcaaaagcaa accaaataga ccgtgccccg tatttaatat atggaatatc acgattttgt
    26821 actgttataa aacctttgca gtcttcttcc cgggttatta gagcaaactg tttttccatt
    26881 gcttcttgta tttgttcatt taaaggatat aaataagcct ctaacaaggg ttgtcgacct
    26941 aaacgataat ctcgtttttg attaagattc agaacctcac aatctttttt aataatctcg
    27001 atagccggaa taaatctctc tctatgtacc ccatttaaat acaactcatc cgggtgagta
    27061 ttcgaagaga taaccaaaac aacaccgtag cgatgcagtg cttgtaataa ttcagccaaa
    27121 atcattgcat aagccacatc gtgaactaaa aactcatcaa aacacagtaa acgaatcgat
    27181 ttggccattt ctttggcgat atactgcaaa ggattttttt tgccttgtaa tcgtcttaat
    27241 tgagcgtcaa tttgctgcat aaaatgatga aaatgaaatc tcgccttttt ttcctcatca
    27301 atatgctgat aaaacaaatc caccaaataa gtcttgccaa ctcccacagg cccatagata
    27361 taaagccctt taatgggatg cttttttctc cagggaaacc aagagtcatt cttcttctgt
    27421 aaatcctcag caagcctctg catgtgttca aggatttctc tctgctcagg atcgctatct
    27481 atttcacctc tataaatcgc ggcttcatac tgctcaatca aactcatggc aggcaaacct
    27541 gatcacgaat agcattcatc aacttttctt ttaatattaa cagcttacca tgaaaaaaat
    27601 gacccgtttc aacaaattca atgactggca atacctgcga taattggctg gcgaagttac
    27661 tcaccagttc aaatggcacg acctcatctt ctttgccttg tataataagc cagggatgag
    27721 gaaggggttc aaattctttg taatcataat gatgcacagg aggagctatc gttattaaca
    27781 gagcatgctc acaatgagac gccgttctat aagccacata agatccgaaa gaaaagcccg
    27841 caaaaatatt ttgggcttgc ggttgctcag aatgccactg cctcacgagc ttgcgcatat
    27901 cttcactctc gccaatacca ttatcataat ggcctccaga ctgccctacg cctctgaaat
    27961 taaaccttac tgaaggaata tgcaactcct taaaagctct ggccatcgtg gtaactactt
    28021 tgttattcat tgtcccacct tgtaaagaat gtggatgacc aagaaaagca acataacgag
    28081 tatccgcatc ttcaggaaca gtcagtacgg cttccagtgg gccaactaat ccttcaagca
    28141 ttaattcatg ctctccggga gtgtttaatt tgtcggtaaa tagcataaca accctgccaa
    28201 tttaaagata ataacccaaa tcaatatgga tttaagcaaa agaattaggc tattatagca
    28261 atggatgcgt tgacccccaa tagacctttt gtatatctgg tttattttgt tggcagcgac
    28321 ttttattgac cttcagagga ggacttgatc catgagacag gtacctgtat taccatcatt
    28381 cttatctatt ccaggcgcat tctcctattc agggagagcg gcatgaaaat ccatgatttt
    28441 aaaataaaaa agcaggaaca aaagaaaatt tccatgttga cctgttacga ttacccttca
    28501 gcatgcatag tagcagaatc aaatatcgat tgtgttctcg tgggtgattc cgtagctatg
    28561 gcagtgcacg gccatcctac aaccattatg gccactatcg aaatgatgga attacatacc
    28621 caggcagtag ctcgtggttt aggcaaacag tttttgatta ctgacttacc ttttctcgga
    28681 cataaatcat ctcaaggtca tacagtagaa aacgtaaaaa ggctgttaca ggctggagct
    28741 caagcagtta aaatcgaggg cgctgataaa gacacctgcc aaacgatttc ccatttagtt
    28801 aatgccggaa tccctgttat gggtcatatc ggcttaactc cccaatccat tcatcaatta
    28861 ggcggttata aagtccaggg aaaaaacagc gaacaggcag aaactctatt acaacaggca
    28921 gacgccctgg aacaagcagg ctgctttgcg gtagtcattg aatgtgttcc gcagggcttg
    28981 gcaaaaacga ttaccgattc gttggttatt cctactatag gaattggcgc aggcccaggg
    29041 actgatgggc aagtccttgt ctggcacgac atgctgggtt tacagacttc atttaatcct
    29101 aaatttgtaa aaaaatattt tagagccaaa gaccatttta ttgaagcctt aaacagctat
    29161 gtccagcaag tacaacaaat gcatttccca gctaatgaac attctttcta ggaaaattat
    29221 ccatgcaaat ttttcataat ttaaatgagt ggattcgctt tagaaatacc ctgtcccctg
    29281 atttatccct ggggtttgct ccaactatgg gtaacttgca tgctggacat gcttctttgt
    29341 ttttagccag tagcaaagaa aatcactata ccgtctccag tttgtttgtt aacccgaccc
    29401 aatttaataa ccccgatgat tacaagcatt atccacgcac agtcgatgct gatctcgaat
    29461 taatgactca gaatggagtt gatttttgta tactccccaa tgaaaatgaa atctataccg
    29521 atggctatgc ttatcaagtc caagaaaacc ggcttgggca attgatggag ggaaaacacc
    29581 gaccggggca ttttaacgga gtcctgacca tcgtcatgaa actctttaat ttggtcaaac
    29641 cgacccgtgc ctattttggt gaaaaagatt accaacaact gttgctaatt caaggaatgg
    29701 taaaagcatt gttcatggat attgaaatca aatcttgccc cacagtaaga gaaaaaagtg
    29761 gcctggcctg cagctcacgc aataacaggc ttactccaag ccaaagagaa atcgctgatg
    29821 aatttgcaaa aatatttcat caaaacaaat cctctgccat gataagcaaa gaattggaag
    29881 cgcttggaat tactgtagaa tatattgaag aattccaggg cagacgattt gccgcagtaa
    29941 aaattggcga tatccgtctc attgataatt atttgctatg attcaaacca attggtgcgt
    30001 gataacaggt ggaccttcct cagggaaaac cactttaatt aatcatttgg cagagttggg
    30061 atacaaaact gcgccagaaa tagccagatt ttatatcaac cagctgctgt ctcatgataa
    30121 aaaaactcta caagaagtaa aacatgatat cctttggtta caaagaaaaa ttctcaatat
    30181 catgttaaga cgagaaagaa acctgcctaa ggacgactta attttttttg accgtggcac
    30241 cccggacagc atcggctatt ttcgttttca tcatttagat gaggctcatg tggtcagagc
    30301 ttgccagtat ttacgctata aaaaaatttt tttctgtcat cccttgcctg ttgtccctga
    30361 taccataaga gatgaggatg atgtcaccgc aaagaaaatt agcgattaca tttataatgc
    30421 ctatgttggc cttaactacc cagtcataga attaccagca acctcaataa aagaacgcct
    30481 ggaaattatt ttatcccata ttgataccta gagataaacc tgttctaggt tttattcaaa
    30541 gcgattgccg ctgcaataac cgctgccttg tgctgaggga aggatttatt tgagtacttt
    30601 aaccattcgg ggtgtgcctc cactaaaccg gtattgaata cccctttctg aaactcctct
    30661 gattttatca tttctaaata ataaggaatg gtagttttta ctccaaacat gcgcatttcc
    30721 tcaagagctc gacgcgcccg tcgaagtacc gagggccaat caaaagccca tacggtcaat
    30781 ttggcacaca aagaatcata atccggggga atctggtaac cagtataaat tgcgctatct
    30841 gtcctcacac cgggccctcc cggggcgtaa tatcgggtaa tccgcccaaa actgggtaaa
    30901 aaatcgtttt gagggtcttc tgcgttaatg cgaaattcga tggcaaaacc attgtattta
    30961 atttgttctt gagagatagt tagacgctca ccagctgcaa ttcgaatttg ctcctggact
    31021 aaatcaatac cagtaatctg ttctgtaatt ggatgttcaa cttgtaaacg agtgttcatt
    31081 tccatgaagt aaggttgatt atttttatcc aaaataaatt caacagtacc cgcattggta
    31141 taccctacct ccttggctgc cttgactgcg tatcccaaaa gcacttgacg tgtcgcttca
    31201 tccaattggg gggaaggcgc tatttcaacc agtttctgat gtcgcctttg tattgaacaa
    31261 tcccgttcaa acaaatgaag tactgttcca tgatgatctg ctagaatttg tacttcgata
    31321 tggcgtggat tcgctatgta tttttccatg tagatatggg agtctccaaa tgatttttct
    31381 gcttcagatt gcacgcgagc aaactgttgc tttatctgac tggcatcatg acataatctg
    31441 atacctctac ccccccctcc aaaagtcgct ttgagcatta caggataccc tagagtttct
    31501 gcgcaatgta aagcgtcatc tatagtttcc aaattcccct tgcttcctgg tatggtggga
    31561 acccctgctt ttatcatcgc ttctcttgct gcaatcttgg agcccatagt ggccatcact
    31621 ggagcagacg gtcctataaa aacgatacct gccttgttac aatcctcagc aaactcggca
    31681 ttttcagata aaaaaccata cccggggtga attgcttcac aaccggccac cttggcgcac
    31741 tcaataatac gccgtccatt aagataagca tccagaggtt ttttactaag acagtgagaa
    31801 tgagaagctc ttttgacatg cagggcaaat ctgtccactt cactgtgaat agtaactgct
    31861 gctatcccca tctccttgca agcacgtact attcgcaggg ctatctcacc gcgattagcc
    31921 accagaattt ttttaaacat taaaaatctc ttgtaaaaac tggctcattg cttgccatga
    31981 acggcgcgca gcaacctcat cgtaaattaa tcctaaatgt tcatcatgcg cttgaggatt
    32041 agtaaaagca tgtttcacct gcccatacat atgcatttgc caatccacat tagcttctgt
    32101 catttcctga caaaaagcat gtacttgttc cggtttcgcc ataggatcat cataaccgtg
    32161 caaagccaga atttttgctt taattcttcc ggatgctaga ttctcaggct tattaagcaa
    32221 accatgaaaa cttactgctc ctcgaatatc cgcaccactc ctggctaaat caagggcaca
    32281 caagccacca aagcaaaacc ctataatggc tatgcgattg ttatcgacta ccgatattga
    32341 tttaatggct tccaatgcgg catacaaccg ctccctaagc aaagagcgat cgctgtacaa
    32401 gggttgcatt aatgccattt tttcatcatt gttagaacca atttgtcctt gcccatacat
    32461 atccaaagca aagcctatat agcccatctc agcaagttgt tttgctttaa ggcaagcaaa
    32521 ctcacctctt ccactccaat catgagcgac tattacggca ggccgagatt gtttaattcc
    32581 ctcttcatgc gctaaaaatc cgtgtaactc aagatcatca tgttgataaa tataattcga
    32641 tgaatacata aaatatctcc taaattattt taaaatagtc tagggcaata tccgtaaaag
    32701 tccagtaatt acattagttt agcgtaatgc tattctttgt cttttgttcc tcgtggctaa
    32761 gtaaaaatag tgtcgtccaa taaaaggata tcaaattgaa ttaatctaat gctcttgcat
    32821 gtttcgccaa aacatgagtc catgagtcag taaatatcaa tatgtccact aattaactca
    32881 aattatggat ttggtctaaa ataaaaataa attatgtgag gaatgttgcc atgatgactg
    32941 attcgttaat acacataagg gatttgttac atgctcaatt ttgctaccaa atgtttatta
    33001 cccccattca tcttcctatc gataaaaaat acagggaatt tgccaaaatg gcctgtgaat
    33061 tcatagaagg aagacgggat gaaatcatac acagagaata tcctcgtcat catgttttac
    33121 atcgatttac tcccgcgaac aaaccaaatg gaaaaaaaat attgattacg catggctgga
    33181 tgtctcgtgc tgcttatatg gttcgcttaa tcaggcttct tcataaagaa ggatatgaag
    33241 tctatgctat cgattttcct gcccatggcg aagctaaagg aattcagctt ccctgggctg
    33301 atgccattgc tattatcaaa gaaacgatta accaatttgg gcctttttat ggattggttg
    33361 gccattcttt tggtggttca atgattttaa atactttgaa tctggcaggc caactaccag
    33421 agtggcaatt aaatcacaag ccggaaagag ccattttaat tgcatcgcca acacaaatgc
    33481 gaactcctgt caataaaatt gccagacgtt ttaaattaag cgggcaagcc tatttacaac
    33541 ttcgccaatt aatccgtcaa caagcatcgg tagatcctga gagaattcgt ttaagtcatt
    33601 ttatttccca agcccctgat acctcttttc tatgtatcca tggagaattg gatgccacta
    33661 tcaaccccaa agaatcgata aatttttgca aatattacaa aaatgcccgc ctgtctcttt
    33721 taccagacgc tgatcatgtg agtgtattaa tggatgagcg tgtcgaacaa atcgctttgg
    33781 attttttaaa tccttaatat gacaggggaa ttttgatcca cctggattac ggctttacct
    33841 aatccaggtg gttccagtag ataggcagac cattgtattt gctgaagcaa tgtgcttcac
    33901 atacagagga attggcttca aaattttgta attaaccttc catgtattga cggatttttt
    33961 tcatggcatt tttttcaagt tgcctaaccc gttcagcaga gacaccatat ttttctgcca
    34021 aatcatgtaa cgtgagtttt tcttcagcaa gccagcgttg ttgtaaaata tcctggctac
    34081 gttcatccag ttgttccata gcaaacagta atttttcacg cccctgatcg cctgtatcct
    34141 cattctctaa caagacagca ggatcatcgt tcacattaaa caaataacgc tcaggcgctt
    34201 tgtaagcatc atcattatca tcaacatctg gcgcatcata agatgaatcc ataacattta
    34261 aacgttgctc catcaacaac acgtcttctc ggctaacgcc caaatctttg gcaacagcgt
    34321 caacttcttc attgctgaac caacccaatc gatttttcat ttggcgcaaa ttaaaaaata
    34381 atttacgttg ggccttggtt gtagcaactt taactattcg ccagttacgc agcacaaact
    34441 catgaatttc tgctttaatc caatgaacag caaaagaaac aaggcgtacg cccatcttgg
    34501 ggtcaaaacg ttttacggct ttcatcaagc ctacattacc ttcctgaatt aaatcactta
    34561 aaggcaatcc atacccaaga taaccgcggg ctacacgaac tacatagcgc aaatgcgcaa
    34621 gaactaattg tcgagcggct tctatatctc cttcagaatg aaaacgctcg gcatatgcaa
    34681 tttcctcttc caaagtcagc attggaattt gatttactcg atggatataa gaatcaagac
    34741 taccaacagg tagattcatt gcagcaagtt gcaactgttg actcatacta cttcctccgg
    34801 tcattaacac tagaatagta ttaaaattta catcctatta actgatag
//
LOCUS       DATNSN010000016        33936 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.016, whole genome shotgun sequence.
ACCESSION   DATNSN010000016 DATNSN010000000
VERSION     DATNSN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 33936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33936
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.016"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1790)
                     /gene="oadA"
                     /locus_tag="VAH76_000626"
     CDS             complement(<1..1790)
                     /gene="oadA"
                     /locus_tag="VAH76_000626"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HEO1385274.1"
                     /translation="MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAME
                     VWGGATFDACLRFLKEDPWSRLRQLRQALPNTQLSMLLRGQNLLGYRNYADDVVRAFV
                     KLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
                     KKLAEMGCDSIAIKDMAGLLTPTVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEA
                     VLAGCNHIDTAISSFSGGASHPPTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKK
                     YSQFESEAQNIDPRVQLYQVPGGMISNLYNQLKEQNALDKMDAVHKEIPRVRKDLGYP
                     PLVTPTSQVVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRT
                     EVIEVRPGDLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEP
                     LLTQSSAPDNSVMSEFDIILHGENYHVKVAGYGMIEHGQQSCFLWVDGVPEEVVVQHS
                     ELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSAGDEVKAGQAVLVIEAMKMETEIK
                     APANGVVAEILCQKGDKVTPGQVLIRVEVS"
     gene            1992..2429
                     /gene="aroQ"
                     /locus_tag="VAH76_000627"
     CDS             1992..2429
                     /gene="aroQ"
                     /locus_tag="VAH76_000627"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEO1385275.1"
                     /translation="MKKILVLHGPNLNLLGSREPSIYGHASLTEINGDLIQEADNAGI
                     RLSCFQSNAEAELIQAVHQAGIDKINYIIINPAAFTHTSIALRDALSAVAIPFIEVHL
                     SNIFSRETFRHHSYFSDIAVGVISGLGTKGYLLALQAIIKELK"
     gene            2442..2924
                     /locus_tag="VAH76_000628"
     CDS             2442..2924
                     /locus_tag="VAH76_000628"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO1385276.1"
                     /translation="MDIRKIRKLIELLEETGISEIEIKEGEESLRLSRHSNIVAETAP
                     QIHYVAPSAVKQTASAPSSNTSTTEPHVVENKQVTSTSSGHKIRSPMVGTMYTAPSPE
                     SPPFVTVGQSVKAGDTLCIIEAMKMFNEIEADRAGKIVEILVKNGDPVEYDQALFIIE
                     "
     gene            2937..4280
                     /gene="accC"
                     /locus_tag="VAH76_000629"
     CDS             2937..4280
                     /gene="accC"
                     /locus_tag="VAH76_000629"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO1385277.1"
                     /translation="MLSKIVIANRGEIALRILRACKELGIQTVAVHSDVDKDLLHVRL
                     ADETVCIGPAPAQKSYLNIPAIISAAEITDAVAIHPGYGFLSENADFADIVEQSGFRF
                     IGPRGDSIRLMGDKVSAIAAMKAAGVPCVPGSDGPLGDDDNKNLELARKIGYPIIIKA
                     AGGGGGRGMRVVHSESNLLSAIALTRSEAKAAFNNPIVYMEKFLENPRHVEFQVLGDG
                     KGHAIHLGERDCSMQRRHQKVVEEAPAPGITPELREEIGASVIKACKELQYRGAGTFE
                     FLFQDGCFYFIEMNTRIQVEHPVTEMITGIDLIKEQIKIASDIPLTLTQKDIKLRGHA
                     IECRINAEDPRNFMPSPGTIKLLHQPGGPGVRFDSHIYSSYVVPPNYDSMIGKLISYG
                     ATRAEAIARMRNALDEIIIDGIKTNIELHQRILHDKSFMEGGTNIHYLEKMLKDS"
     gene            4282..5151
                     /gene="prmA"
                     /locus_tag="VAH76_000630"
     CDS             4282..5151
                     /gene="prmA"
                     /locus_tag="VAH76_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO1385278.1"
                     /translation="MWFQLKIEHCPNDKIEEITEELEEYGALSITLTDKNDNPVLEPE
                     PGTTPLWPEVIIHALFAQAEEAQYVREQLVAKTPSLHCSLEPLADKNWERAWMDDFRP
                     QRFGNRLWVCPTWLPPPEPDAVNLMLDPGLAFGTGTHATTSLCLTWLEQADLKNKSVI
                     DYGCGSGILSLAAIKLGAKHVYAVDIDNQALQATQNNAHANHITESQLSISSPEALQN
                     PVHLIIANILLAPLISLKERFHQLLPSGAHLVTSGILEEQAPLLIDAYDSAFTHIATE
                     YCEGWSLLVFTSK"
     gene            5178..6767
                     /gene="purH"
                     /locus_tag="VAH76_000631"
     CDS             5178..6767
                     /gene="purH"
                     /locus_tag="VAH76_000631"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO1385279.1"
                     /translation="MAKKQIYSSFKPRRALLSVSDKRGIRELGQALHDHGVELIATGN
                     TAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLKQHGIKP
                     IDLLVVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
                     LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFN
                     KITDLRYGENPHQQAIFYADKNSHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFS
                     NEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAIL
                     EKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHD
                     SLSLESCELQTVTQAKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSR
                     VMSARIGLWQAEQMGFDPQGAVMASDAFIPFPDTIEIAAKAGISAIIQPGGSIRDEKI
                     ISCADQHNIAMIFTGVRHFKH"
     gene            7052..7837
                     /gene="icmH"
                     /locus_tag="VAH76_000632"
     CDS             7052..7837
                     /gene="icmH"
                     /locus_tag="VAH76_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEO1385280.1"
                     /translation="MTTEQYPSSLVNRLAITEPALVPSGYYRSKLFIAPFSTNVLVAA
                     AGPILSLLERLCLSPSLPPVEDIRENIQHELRAFHSKLDASKYPLDIISIAQYLLSAT
                     IDEILGKSYLRVYNLTTEFKSFTPLSSDGAQPQQRFFEILNYIKERPNQFLDLIELAY
                     FCLIAGFEGEYHLKADGRQCLDNTIEDLYQIIQKYRFNKPHRLFNENPLPKTIKKNYK
                     ATWITTIAAVGIVILAFVTSHVLLENKAKTVLFGHTQLAMLEN"
     gene            7837..10758
                     /gene="icmF"
                     /locus_tag="VAH76_000633"
     CDS             7837..10758
                     /gene="icmF"
                     /locus_tag="VAH76_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmF"
                     /protein_id="HEO1385281.1"
                     /translation="MDNSLRALCDAIKKILSQLKPQANQLSFIVITGKNAQGKSALLK
                     QSNMEEMPVFSEEHAKLYFNQKGIIVELGENWLTNSKTLLLTTLKQLNKCNRHLKITG
                     LILCVDVNDLLIAEPAQFTEQKKSHLQLIQRLGVNLGYQAELAIIFTKMDTLAGFSEF
                     YQMDHVNDLSKPLGFSLDCVNQSSKKIETFSLQFNQLIEALGQQVINKMHPVRSTIKR
                     SLIREFPLQLASLRAPIQSLIQGISPKLFHVHSIFFTSAEQGGVSVDRLNKKIQHEYA
                     LVVQDTFPQATNFRSYFVEGALKTIQEQCIQVPQTTKFSQKPFIAVAVSLAGLSLLTL
                     IYNHYKTAHILDEASKELLAYEALNNQGTSGAQALYHLSQAANKMNHITSNSISLPTV
                     HQLKLNLHNNAQNRLQGEFLPSLANELEQVITSPGNTPITRYKALKIYLMLGDSKYFS
                     SQPILDWFQQQWQGRAPGTTQKQLALLKQTLSNPPQNIPIKSQIISDARNYLNALPTS
                     YLYYSIAKEYFPQEKQKIVIEGFSIPTDEVPTYFTKTGFTKIINELPKITSTLQSENW
                     VLARQDLSQLHNMLIQAYCFDYVTWWQTFMKKSQPLRYQDYQQGRQLTKLIQQSNSFY
                     KFANLIQQETKPDLTEGISTFNQFIANQFTDLNLTTLSSIKELDFRISELERFIATLS
                     VVNDGGKTAFTITRSRFLNENSSDPVSLLYSQARQLPEPLSSWTKQIAGDIWVILIRD
                     SRQYINSQWQKTVYREFQNSIAQRYPFDSSQTAEIDIADFNRFFSTHGLLNTYSEQYI
                     KPFLDTSSPEWKPKAVNDFILPISNEVIDELIRANIITNMFFPDHSDESKIDFSLQKI
                     SLDPVVSNLVLEIGGTKLMDNQTSDSLIRFTWPQNNAKLALDSIEGNHYELAEYGTWA
                     IFKLLEKVNVLVDEQDSSSLQILFEVNSNSGRYLLKTNNQINPFTPGILNGFTLNDHI
                     A"
     gene            10761..12017
                     /locus_tag="VAH76_000634"
     CDS             10761..12017
                     /locus_tag="VAH76_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385282.1"
                     /translation="MNQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHF
                     VSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKALILSMSVMTISTALIAVLPDFN
                     SWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
                     GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAEL
                     PAKVVFTRFKQQLFLSVIITSILAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALA
                     LLTVLIPLMGFLSDVVGRKPIFLIGLFSLCILIFPFFWLLLSGSWWKALCSELLLAIA
                     LAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPA
                     WYLFLWILIVIPITISIRESYQKELT"
     gene            complement(12163..15192)
                     /gene="icmB"
                     /locus_tag="VAH76_000635"
                     /gene_synonym="dotO"
     CDS             complement(12163..15192)
                     /gene="icmB"
                     /locus_tag="VAH76_000635"
                     /gene_synonym="dotO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmB/DotO"
                     /protein_id="HEO1385283.1"
                     /translation="MANWSESFFEGVDTFFAWLSTSLKQTTESYIDLETADSPTVLVN
                     HDGSLLSVLKIEGITGLAGPEEFERLVEGLANSFQAAMGRPGHALQVYFSHDKQNIVK
                     MIKDIYDPAEATASRLELNLRDLFEERVNYLSQYCAEERVYFVLYTRPFNLASDQLKA
                     ANKAKMKMIKDTKAPPFKNTQTIFAAVPEIRDTHDAYVRAILNDLDALNVIAKLLEVH
                     DAIHAIRMTGDPDYTADDWRATLPGDKIPVRELNNFEGDPSDLLWPPLSKQVLPRDAE
                     IIDLRTVRVGDKIYSSTYIDLFPKDVRPFISLFSRILPSHVPWKISFLLESEGLSTIK
                     LKGLLAAILSFSSAQNRLISDSVNLLKYIQLNTDEAIVRLRVVATTWAPEGNIPLLRR
                     RSSELVKAIEGWGSTDVSEICGDPFAGFVSSMLATTLNSSAVASVAPLSDVISMLPIT
                     RPASPWKTGALLFRTPDGKPWPFQPGSTEQTTWIDLVYARPGSGKSVLSNALNLALCL
                     SGGLMRLPRIAIIDIGPSSSGLISLLKEALPASKRHLVAYHRLRMTPDYSINPFDTQL
                     GCRYPTALERSFLVNFMTLLTTPLGAAKPYDGMADLAGMVVDELYKSMADEFNPTPYA
                     PGIEEFIDSILEEIGFVRDAKSTWWEVTDSLYSAGFVHEAMLAQRYAMPLLADAASIC
                     RTPSIEDLYEKITAPTGESLINAFSRMISSAVREYPILSRVTSFDIGDARVVSLDLDE
                     VAKSGGDAADRQTAVMYMLARYVLARHYYLTEESLNNVPEQYKEYHKQRVLEIREDHK
                     RIVYDEFHRTAKSSAVREQVIIDMREGRKWKVQISLLSQAVDDFDPVMIDFATAIYVM
                     DAGPSQAVEKTSQIFGLSETAKIALRTRVHGPRQGGATFLAQFATKTGINVQLLTLTL
                     GPVELWAFSTTAEDATVRNLLYRHLGPSEARRLLAALFPNGTVTKELEARLASMKQKV
                     GLIEDDEREGMINQLVNDILDAYSKDPNVKSLPAKVG"
     gene            complement(15228..15854)
                     /gene="icmJ"
                     /locus_tag="VAH76_000636"
     CDS             complement(15228..15854)
                     /gene="icmJ"
                     /locus_tag="VAH76_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HEO1385284.1"
                     /translation="MADNQQRCELKLIASPGSWRLYSARKIDERFKSYEQKIFQRDRY
                     TCQFCGFQARLYQDIVNLDGDYTNNRLSNLVTACCFCAQCFFVESVGVGGYGGGTLIY
                     LPELTQAELNSLCHVLFCAITNDTGYKSSAQNIYRSFKFRSQIVEEKFGEGTSDPAIF
                     GQLMIDSGVNSEEIREKLFKNIRLLPSRAKFRKQIEKWAASALEEIAD"
     gene            complement(16054..16452)
                     /gene="icmD"
                     /locus_tag="VAH76_000637"
                     /gene_synonym="dotP"
     CDS             complement(16054..16452)
                     /gene="icmD"
                     /locus_tag="VAH76_000637"
                     /gene_synonym="dotP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmD/DotP"
                     /protein_id="HEO1385285.1"
                     /translation="MNIKVIHKSGYKHWLVSAACLALLTLICQDAAAGVSLGKMASQI
                     TGSFTSLTKLITAGSYLAGLGFSIGAIMKFKQHKDNPTQIPIGTPIALIFIAAALLFL
                     PSILGVAGVTMFGSSGGKTAGPTGTIYKTN"
     gene            complement(16478..17062)
                     /gene="icmC"
                     /locus_tag="VAH76_000638"
                     /gene_synonym="dotE"
     CDS             complement(16478..17062)
                     /gene="icmC"
                     /locus_tag="VAH76_000638"
                     /gene_synonym="dotE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmC/DotE"
                     /protein_id="HEO1385286.1"
                     /translation="MADPTCTGGTVACWIVSQANILANIANQLEPVQRLITGGAYLIG
                     CAFIFKAIYSLKVYGEARTMMSSNTSMKEPVMYLMVGALLIYFPSLVSSVLQTTFGYS
                     NPLAYSGGVSSGSDTISALFGSGSLVGRPLVMIIRVIGLIAFVRGWVLIARSASQGQP
                     PGGTGKGLIHVFGGILAINIVGTVDMINNTLYGT"
     gene            complement(17070..17879)
                     /gene="icmG"
                     /locus_tag="VAH76_000639"
     CDS             complement(17070..17879)
                     /gene="icmG"
                     /locus_tag="VAH76_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmG/DotF"
                     /protein_id="HEO1385287.1"
                     /translation="MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASFQSGKEGLT
                     KKKDIKRNALIAIGAVVFIMVMYKIIGWMFFSDKSSQVTTKPAIPPVTQVATPQPVQT
                     IPTTTPIQQVQPTTIIEDDPDLKKKVSAIELTQQSLRSEVNALGEQINAVNNNIKNLN
                     AQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPTVPVRIIYYIQAVIPG
                     RAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS"
     gene            complement(17894..21040)
                     /gene="dotG"
                     /locus_tag="VAH76_000640"
     CDS             complement(17894..21040)
                     /gene="dotG"
                     /locus_tag="VAH76_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotG/IcmE"
                     /protein_id="HEO1385288.1"
                     /translation="MASKKENLKSLFSNTRTRVIIIFTAALLIIAVVIGFFKIRSATT
                     SSIAAAEVSTVPGGIQSIPGVLDPTAQYAKLQEEQNITQAQVAEKTGGSAIPTIIRTQ
                     ALGEGVGVIGSQSGVGFAALAQEELGGPQRSLWIQELQDGGCSKSVITKVVNQGAQLT
                     DLKAACSCVQLKDSGYGLQELEQVCECKELKAAGYNARQLKEAGYSAGRLRNCGFDAC
                     ELRNAGFTAQEMKDGGFSDGELKGAGFSDAEIAKASGLPDGITADDVRKAGCGAAALA
                     KLRQAGVSASAIRKISGCTAEQLKAAGYTAKELKDAGFSAADLKRAGFSAAELKDAGF
                     TARDLLNAGFTPADLAKAGFSNAQIKAAQAELPPGITPQDVKNAGCDVEALKKEREAG
                     VSAALIRQYAGCSAQALKAAGFTDADLASAGFTPAQISAATPLSDAEIKAAGCDPDKL
                     KKLFSAGVSAKRIKELNGCSAEALKAAGYDAQSLLAAGFTPQELLAAGFTPKQLEDAG
                     LNPASIIADGRVADCSVESLKKARAAGVSALTIKQTLGCSAAALKAAGYTAKELKDAG
                     FTAAELKAAGFSAKDLKDAGFTAKELRDAGFSAQELKDVGFSAKDLKDAGFSAAELKA
                     AGFTAAQLKAAGFSAKDLKDAGFSAAELKAAGFSAKELKDAGFSASDLKNAGFSAKEL
                     KDAGFSASDLKNAGFSASELKNAGYSADELKKAGYTSAELRNAGFSPQESAVSGLQGP
                     DLQQLDSSITGIPSIPGATPRPTTSDAASSAEQLQAILQKQNEQLAEQKYQQEIQQRT
                     SDMLSAATQLVQDWKKVETQVYTEGTEETKTSGGESSVPGAGTGAGANNQPVEQGASG
                     AQNQAIIKTGDIMFAVLDTAVNSDEPGPILATIVTGKLKGSKLIGSFNLPSNADKMVI
                     TFNTMSIPGAEKTISISAYAIDPNTARTALSSRTNHHYLMRYGSLFASSFLQGFGNAF
                     QSANTTITIGGTGGGNNITVANGVGRSTLENAVIGLATVGKAWSQQAQQLFNTPTTVE
                     VYSGTGLGILFTQDVTTI"
     gene            complement(21045..22127)
                     /gene="icmK"
                     /locus_tag="VAH76_000641"
                     /gene_synonym="dotH"
     CDS             complement(21045..22127)
                     /gene="icmK"
                     /locus_tag="VAH76_000641"
                     /gene_synonym="dotH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmK/DotH"
                     /protein_id="HEO1385289.1"
                     /translation="MKKYDQLCKYCLVIALTFSMSCSIYAADQSDDAQQALQQLRMLQ
                     QKLSQNPSPDVQLGAGDGGDNAASDSTQQPNQSGQANAPAANQTANVGGDGQIISQDD
                     AEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKASTAGTPPKPTATSQFVNLS
                     PGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATK
                     LYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSAN
                     DLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTH
                     AYEMQKSPVLLVSWHGKVMQLKVEGL"
     gene            complement(22130..22768)
                     /locus_tag="VAH76_000642"
     CDS             complement(22130..22768)
                     /locus_tag="VAH76_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system apparatus protein
                     IcmL/DotI"
                     /protein_id="HEO1385290.1"
                     /translation="MAEDALTVVALRNKFYKDSQRKVILALLIAIVVNVVLASLVVYM
                     ITHPPAPKYFATSINGRITPLFPLDEPNQSDSAVLQWANQAAIAAFTYNFVNYRDELQ
                     ASSGFFTAEGWDQFLGALEQSNNLDAVKAKKLVVSAVATRAPIILQKGVLNGRYSWRV
                     QMPILVTYQSASEFTQQNNVVTMLITRVSTLNSPRGIGISQFVVGPASGGVS"
     gene            complement(22784..23068)
                     /gene="icmM"
                     /locus_tag="VAH76_000643"
     CDS             complement(22784..23068)
                     /gene="icmM"
                     /locus_tag="VAH76_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmM/DotJ"
                     /protein_id="HEO1385291.1"
                     /translation="MSRETWALIKRNKNFNVHIYRRGLTLLILSLGLSCGLSGLIYYV
                     HIHEPERDFYATSGITPPIQLKPMSTPNMSSEALLPPDPPDEDSQRIIPQ"
     gene            complement(23080..23649)
                     /gene="icmN"
                     /locus_tag="VAH76_000644"
     CDS             complement(23080..23649)
                     /gene="icmN"
                     /locus_tag="VAH76_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmN/DotK"
                     /protein_id="HEO1385292.1"
                     /translation="MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCR
                     VDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEI
                     AAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLG
                     SDKPITSYTLGGDRSPNARVEITFRRAVA"
     gene            complement(24053..26404)
                     /gene="icmO"
                     /locus_tag="VAH76_000645"
                     /gene_synonym="dotL"
     CDS             complement(24053..26404)
                     /gene="icmO"
                     /locus_tag="VAH76_000645"
                     /gene_synonym="dotL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmO/DotL"
                     /protein_id="HEO1385293.1"
                     /translation="MMRGIDSRHELDPTLLLRDTRTFTQRLADFFADPTNISIVLISL
                     AAVSYYFSEAATFLLIMGSIFFLYSYTRKQKLPFRLPQISRAKDYNDLKPGINKPNIA
                     RGITFFGNDRKTGEELWFANDDMRTHALIFGSTGSGKTETLVSLSYNALVQGSGFIYV
                     DGKGDNSLYAKVFSMVRSMGREDDLLLINFMTGARDIVGPQEKRLSNTLNPFCQGSSS
                     MLTQLVVSLMGSSGQSSDGDMWKGRAIAFVEALMRLLVYMRDEGAILLDANTIRNYFD
                     LQRLESIVIDKVFPRDDQESVNIETIPKLVTDPLRNYLNTLPGYNKEKKGKQVSQVLE
                     QHGFITMQLVRSFSSLADTYGHIIRTNLAEVDFKDVVLNRRILVVLLPALEKSPDELS
                     NLGKIIVSSLKAMMAAGLGEEVEGDYRDVILRKPTNAPTPYMCILDEYGYYAVQGFAV
                     VPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKLEDPTETWDFFTKT
                     AGEAYVTKVDSFQTKETSIANSYMDTKSSSFEKRARVDLLDLKEQTEGEAHIFFKSKI
                     VRARMFYANPKPVKQLKINQFLKVEPPPDDYLMKLQKQLASFQSILESGDLSINNAVE
                     NEEITLISKALKESTIVEPIERGVAALIAFHGQNEPEPVEDIVEEEVEGALTIFSKLR
                     IDPNAPPILVADKEVFSEPLLPINETRNQMITIECLAGAKDKYAGTVANELIKDFQIA
                     TSYPPEERDVIDVQELTGIIRDLSAKISAEREKANKKAAEELT"
     gene            complement(26401..27531)
                     /gene="icmP"
                     /locus_tag="VAH76_000646"
     CDS             complement(26401..27531)
                     /gene="icmP"
                     /locus_tag="VAH76_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system coupling complex
                     protein DotM/IcmP"
                     /protein_id="HEO1385294.1"
                     /translation="MAQQQQQSGSDNSMAPVWIVILLFITAYFVWALAHQYIVSFVFT
                     INIWQARLVNLFLNNQLLANQIYLMQTLDPNTVNWDQMVTVMRAVGDYMRYPVICILV
                     VLAFVLYNSNVTLKYRKTYDMKSLRAQEQFNWPAIMPIVKEDLVSQDVNKGPWAMALT
                     PMEFARKYNLLRKDDALLDNPVPGQEMTAGIRRGDAKRVFTMQLGPYWDGFERCSPQA
                     YALSAVFMARMNRDRDAANNILKVLDKTFVDGKPDFSVARPVMKKYQNSELVQEVVAK
                     HAYVLTVIASLLEAAREDGVVPSSEFLWLKPVDRRLWYMLNCVGRQTPYSEVAGPFAH
                     WKAEKEMGRRSLVPMIDEAIRALEIAVKEVRLTPRQMEELEP"
     gene            complement(27718..28293)
                     /gene="icmQ"
                     /locus_tag="VAH76_000647"
     CDS             complement(27718..28293)
                     /gene="icmQ"
                     /locus_tag="VAH76_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system protein IcmQ"
                     /protein_id="HEO1385295.1"
                     /translation="MKDQLSDEQKETILKALNDAIEKGPWDKSNFLRVIGKKLIAIRD
                     RFLKRIGAASQAKLKAESHLANRIALRSGQQEIYVSLYSSDGSNLQSWEKIVGSLPRQ
                     MISRPIYADEEDIKAILKTKENKQNEAYVAIYISQSDILHLSADKAPVDKLGKPLLTL
                     KDKSISLENISRFVHVSGVYRYSNGRLIKNA"
     gene            complement(28384..28746)
                     /gene="icmR"
                     /locus_tag="VAH76_000648"
     CDS             complement(28384..28746)
                     /gene="icmR"
                     /locus_tag="VAH76_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmR"
                     /protein_id="HEO1385296.1"
                     /translation="MGNNTDDSARNPFGFYTPPRVKEIGESDVTDATLGSVYSEIISP
                     VKDCILTVAKAVSFNPGGKDNTDAVDVLTELNTKVERAALNQPILTAKTERMFGATES
                     EKSSEQPSHEERGFKLSS"
     gene            complement(28846..29190)
                     /gene="icmS"
                     /locus_tag="VAH76_000649"
     CDS             complement(28846..29190)
                     /gene="icmS"
                     /locus_tag="VAH76_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmS"
                     /protein_id="HEO1385297.1"
                     /translation="MERDISKCMAKIAASMNAKFYLNDRFVSFDEVFSETGLLPAIAK
                     RADQLCSLCLGYGLGATYDESEGALLGIRVVFDEVTPNVLRLLCMTDVMNELIQGGPS
                     RDYTPLDELMYD"
     gene            complement(29191..29451)
                     /gene="icmT"
                     /locus_tag="VAH76_000650"
     CDS             complement(29191..29451)
                     /gene="icmT"
                     /locus_tag="VAH76_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmT"
                     /protein_id="HEO1385298.1"
                     /translation="MAGGFSATSHWRDSARSARFFVVDARAAFPIFLFLMHIRVWTGV
                     LVLVSAIFFGIIEHYGFTVPVFLRWLRSFLAGSVRSARPWWR"
     gene            complement(29622..29834)
                     /locus_tag="VAH76_000651"
     CDS             complement(29622..29834)
                     /locus_tag="VAH76_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385299.1"
                     /translation="MDIHSTYKMLAKMQGNKELSSHKIAETEQAEADTVPQLKLQAEP
                     EAKPDMQNILSNMLGISSNKKHSPMD"
     gene            29926..30975
                     /gene="ceg15"
                     /locus_tag="VAH76_000652"
     CDS             29926..30975
                     /gene="ceg15"
                     /locus_tag="VAH76_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg15"
                     /protein_id="HEO1385300.1"
                     /translation="MTDHSPFIKFRFLNIKLSILQYNIILKAIIKLKVAILMTNCKTS
                     DTLLLKPILGYSCFNFSISVFILILFYIPLTSGTCWAKKSVNHNEVVMRGKREFLKHE
                     YSPGHWSIDYTRAGTSIAVITVRNKYHYSVILNPTDCRGYRIIIRYLNEGDSSLSSAF
                     NRPYTVSEQRGLNDVASLMTQVYEKLGLIVQFSQLGNNSQSFDKETGVTLIGSEEEPS
                     MLHLHMWGRGDPDMEYIAGVPLRGPEPGLMFDLIAKNKTHPINQHAIKWNEDELKACL
                     AMFKLKLAEYVNSPEFTEEFGDTLKVTIHDKKDLGEAKESGFGFFHKMKSASDGITTL
                     EKTLALEDDKINPFV"
     gene            complement(31794..33794)
                     /gene="ceg14"
                     /locus_tag="VAH76_000653"
     CDS             complement(31794..33794)
                     /gene="ceg14"
                     /locus_tag="VAH76_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg14/sidL"
                     /protein_id="HEO1385301.1"
                     /translation="MQNLDEILKKLGEDLKKTSKETANKLSVLSRKVAEDVVKVSDET
                     VQKINEVTKKLSVDTTVSTIDTQRILDDFMKNIRFSRFKQIWQAIEETPKSKHILLKS
                     LFLKGLLTHNKPLLEEIIRQIELIPYSSDLEMLGAFSQDKAHPLSPELLEFVQEMLAN
                     ESEKVLLTILMNAFQSIPELSLDSKTGTLSMKTKKELLPLLIANADTSIAKSIMSQLT
                     DISFDSVLPAFRPSIGDPSYQKTNINLSKYLSLVGDKTDISEFLILTIGLFTSLKIGD
                     KGFLQELSADYLFVRYDDCLHKIIEKLKEKEVIEQLGEEKIQKIMKAVESLQIFNPTS
                     NDPRRFFETRLAQYINGLSHSEKTIEIDSIGEDKEPEDEELRDNTLKACNKILQFFLG
                     DCGRVDTPREMEQKICIFANGYKDTSKFTKKEVEFITGVLHHAEAHKGRDRVERDKLT
                     GIKERKFETDSEKVGSDVWNCGGGVMKGCSPVFYDAQRDPMRNMLVDSSTVAPDNKEG
                     KQWFWNNNRQYSSYVGSISGHTCNIVGMLAKYMENYKEDPDLPNDINLFLIQVIGVYA
                     KRGFHAMLEVIDVLHDPYVQDIFKGYGVQVNLYSYFKENPELAGFLQHAMNDATTYTQ
                     ALVNKKHVKEALKSNSFFSKKDEESGDKKNDHTPKDGTVYGC"
BASE COUNT        10345 a         7259 c         6005 g        10327 t
ORIGIN      
        1 taactcacct ccactctgat taatacttgc cctggagtca ctttgtctcc cttttgacat
       61 aatatttctg caaccacccc atttgcaggt gcttttattt ctgtctccat tttcattgct
      121 tcaataacca atacagcctg cccggccttg acttcatcgc cagcagaaac atgaattgca
      181 ataatagaac cggggatagc tacagtaata tctccaggtc ctatcttgtt attaacagat
      241 gaacgctcta ttttgtcatg aagttcagaa tgttgcacaa caacttcttc aggaacacca
      301 tcaacccaca aaaaacagga ttgttgacca tgctcaatca ttccataacc agctactttt
      361 acatgataat tctcaccatg aagaataata tcaaactcag acatgacgga attatctggt
      421 gcacttgatt gagtcaataa tggctcagga attaattgat tatttttacg ctgctctaag
      481 aattggcgcc caatttcggg aaacattgca taaagaagaa catcctcatc acttagtgcc
      541 aaatcactta tttcattttg caattgatct aattcattag gtaacaaatc gccaggcctg
      601 acttcaatca cttccgttcg tccaatcgct tttttccgta aagcagaact aattttgcca
      661 ggaggagtgc catattttcc ctgacagtag agtttcactt cattggttat tgttttatac
      721 cgttctcccg ttagaacatt aataactgcc tgagttccaa cgacttgaga tgttggcgta
      781 accaaaggcg gataacccaa gtccttacga acgcggggga tttccttgtg aacagcatcc
      841 atcttgtcta atgcattctg ctcctttaac tgattataca ggtttgagat catgccacct
      901 ggcacttgat acaattgaac acgagggtca atattctgtg cctcactttc aaattgagaa
      961 tacttcttgc ggacagcttt aaaataatcg tctatttcca ataaaatatt taaatccaat
     1021 tccgtgtcat acggagtgtc agtcaatgcg gcaaccaatg cttccgtagg tggatgagat
     1081 gcaccgccag aaaaagatga aatcgctgta tcaatgtgat tacaacccgc taaaacggcc
     1141 tcataatggc atatacttgc caaaccggaa gtagaatgac tatgcaaatg tacaggtaag
     1201 ccagtcgctt gttttaaacc agcatacaat tcgacagtca ccgttggggt tagcaaacct
     1261 gccatatctt ttatggcgat actatcacat cccatttcag ccaatttttt ccctagctcc
     1321 aaaaaattgt ccaaagtatg aaccgggctg gttgtgtaac aaattgctcc ctgggcatgt
     1381 tttttatgtg atttaatcgc gtctatggct actttaaggt ttcgcgcgtc atttaaagca
     1441 tcaaacacac gaaatacatc cactccatta tttacggcca atttaacaaa cgctcggact
     1501 acatcatctg cataattcct atagcctaac aagttttgac ctcttaacag catggagagt
     1561 tgagtattcg gtaacgcctg gcgaagttga cgcaatcttg accagggatc ttcctttaaa
     1621 aatcgcaaac aggcatcaaa agtagcgcct ccccagactt ccatagccca aaatcctaca
     1681 tcatccattt tattacagat tggcaacata tcttcagtac gcattcttgt agcgattaag
     1741 cattgatggg catcacgcaa ggtgacatct gttataaacg ttttagacat aagctcttcc
     1801 tggctcaaga aataaataaa aatatcataa tcctaaaatc ggtacactct ttaagtacaa
     1861 tccattgctt tttactaaat ataaaatttc ttgctaaact tgcggagatc ttgccgatat
     1921 cctcgcagat atatagacat gttcataaag ttcaggcaaa atctgcaaat ctcagactat
     1981 accgatgtgt aatgaaaaaa attctagtat tacatggtcc taatctaaac cttttaggct
     2041 cccgagaacc gtccatttat ggacatgctt cgttaactga aatcaatggt gatttaatcc
     2101 aggaagcaga caatgcaggg attcgactca gctgctttca aagcaatgct gaagcagagt
     2161 taattcaagc tgtccatcag gctggaattg ataaaatcaa ttacattatc ataaatcctg
     2221 cagcatttac tcacacaagc attgctctta gagatgcttt aagtgcggtt gccatcccat
     2281 ttattgaggt tcatttaagt aacatttttt cccgtgaaac ttttcgtcac cattcctatt
     2341 tctcagatat tgccgttggt gtcattagcg gtttggggac gaaagggtat ttactggcct
     2401 tacaagcgat tatcaaagaa ttaaagtaga gagaaaaaca aatggatatc agaaaaatca
     2461 gaaaacttat tgaactcctg gaagaaactg gtatttcaga gattgaaatc aaagaaggag
     2521 aagagtcgtt aagactaagt cggcatagta atatagtagc agaaacagct ccacaaattc
     2581 attatgtggc tccttctgct gtgaagcaaa cggcttctgc tccttcctcc aatacttcta
     2641 ccaccgaacc tcatgtggtt gaaaataaac aagttacttc aacctcctca ggccataaaa
     2701 ttcgttcacc aatggttggt acaatgtaca cagcgccatc gccagaaagc cctccttttg
     2761 taacagtggg acaatctgtt aaagcgggcg atactttatg cattattgaa gcaatgaaaa
     2821 tgttcaatga aattgaagca gatcgtgctg ggaaaattgt tgaaatcttg gtaaaaaacg
     2881 gggatcccgt tgaatatgac caggccttgt ttattataga atagaggggc ctgtttatgc
     2941 tcagtaaaat agttattgct aaccgaggcg aaattgcgct tcgtatatta cgtgcttgta
     3001 aagaactcgg cattcagact gttgctgttc attctgatgt agacaaagat ttattgcacg
     3061 ttcgcctggc tgatgaaacc gtatgcatag gccctgcgcc tgcccaaaaa agctatctca
     3121 atattcctgc aattatatct gcggcagaaa ttactgatgc agtggcgata catccaggtt
     3181 atggattttt atcagaaaat gctgattttg cagatattgt tgagcaaagc ggtttccgtt
     3241 ttattggccc acgcggcgac tccattcgtc ttatggggga taaagtttcc gcgattgctg
     3301 ccatgaaagc ggcaggagta ccctgtgttc ccggttcaga cggtccttta ggagatgacg
     3361 acaacaaaaa tctggagttg gcgcgaaaaa ttggttatcc aatcattatc aaagcagccg
     3421 gcggcggtgg tggacgtggc atgagagtcg tacacagcga atccaattta cttagtgcta
     3481 ttgcgttaac ccgaagcgaa gcaaaagccg ctttcaataa tccgatagtt tacatggaaa
     3541 aatttctgga aaatcctcgc catgttgaat tccaagtact tggtgatggg aaagggcacg
     3601 ctatacattt gggtgaacgt gattgctcga tgcaaagacg acatcaaaaa gtcgtagaag
     3661 aagccccggc tccaggaatc actcctgaat taagggaaga aattggcgcg tcagtgatta
     3721 aagcttgcaa agagttacaa taccgaggtg ctggaacctt tgaattttta tttcaagatg
     3781 gttgttttta tttcatcgaa atgaatacgc ggattcaggt tgagcatcca gtaactgaaa
     3841 tgatcactgg tattgattta attaaagaac aaatcaaaat tgccagcgat atacctctaa
     3901 cattaaccca aaaagatatt aagttacgcg gtcatgctat tgaatgccga atcaatgcgg
     3961 aagatcccag gaattttatg ccctctcctg gaaccataaa attacttcat caaccaggag
     4021 gtccaggcgt tcgttttgat tctcacatct acagcagcta tgttgttcca cctaattacg
     4081 attccatgat aggaaaatta ataagttatg gggcgacaag agcagaagcg attgccagga
     4141 tgcgtaatgc tcttgatgaa attatcattg atggcataaa gacaaatatt gaattacacc
     4201 aacgtattct tcatgacaaa tcttttatgg aaggtggcac aaacattcac tatctagaaa
     4261 aaatgctcaa ggattcatag cgtgtggttt caactaaaaa tagaacattg tcctaatgac
     4321 aagattgaag aaataaccga ggaactcgaa gaatatggtg ccttgtccat cacgcttacg
     4381 gacaaaaatg acaaccccgt tcttgaacca gagccaggga caacgccttt atggcctgaa
     4441 gtaattattc atgccctgtt tgcccaagca gaagaggccc aatacgtaag agaacaattg
     4501 gttgctaaaa caccttctct gcattgcagt ttggaaccgc ttgctgataa aaattgggaa
     4561 agagcctgga tggatgattt tcgacctcaa cgttttggta accgcttatg ggtctgtcct
     4621 acttggctcc ctcccccgga acctgatgcg gtaaatttaa tgttagatcc cgggctagca
     4681 tttggcacag gcacacatgc gacaacctcc ctatgcctaa cctggctgga gcaggctgat
     4741 ttaaaaaata aatcagtaat agattatggt tgtggatcag ggatactctc gctcgcggca
     4801 atcaagttag gagctaaaca tgtctatgct gttgacatag ataatcaagc gttacaagcc
     4861 actcaaaata atgcacatgc caaccatatc actgagagtc aactctctat cagctccccg
     4921 gaagcattac aaaatcctgt gcatttaatt atcgcaaata ttttgttagc cccattaatc
     4981 agtttaaagg aacgttttca tcaactgctc ccgtcaggcg cgcatttggt aacatcagga
     5041 attctggaag aacaagctcc tttgctgatt gacgcttatg attctgcatt cactcatatt
     5101 gcaactgaat attgtgaggg atggtcgttg ctggttttta ccagcaaata ataactatat
     5161 cactagggta tatgtaaatg gcgaaaaaac aaatttactc ttctttcaag ccacgtcgag
     5221 cactgctgag tgtttctgat aaaagaggaa taagagaact cggtcaggcc ttacatgacc
     5281 atggagtgga attgatagcc acaggcaaca cagctgcgat attaagagag cataaattac
     5341 cagttaccga tgtcagtgag tgcacgggat ttcccgaaat aatggatgga agagtcaaaa
     5401 cccttcatcc agctattcat gccggtttat tagcccgcgg agaacaagac agccctgttt
     5461 taaaacaaca tggaataaaa cctattgatt tacttgttgt caatttatac ccctttgaac
     5521 aagtcattaa ccacccggac tgcgatttta ataaggccat tgaaaacatt gacattggtg
     5581 gaccagcaat ggtgcgagca gcagctaaaa atcatgctca tacttatgtc atcgttgatc
     5641 ccaatgatta ctcaaaatta attcattatt tacaagatca gaaagcacct tcccattgga
     5701 attttgcgtt agctaaaaaa gcatttgccc atactgcagc ctacgatgcc gcaattgcaa
     5761 attatttaac tactttggat aatgattatg tgccaactgg atttcccgac atactaacct
     5821 gccaatttaa taaaattact gatcttcgtt atggtgaaaa tccccatcag caagctattt
     5881 tttatgctga taaaaattca catccaggct ctctaggtac agcagcctta ttacagggaa
     5941 aacaattatc ctataataat atccttgatg ccgatgcagc tcttgattgt gtgaaatcat
     6001 tttccaatga aaagtcggtt tgcgtcattg taaaacatac caacccctgt ggcattgcgc
     6061 tatccgacac atcacttgat gcttacttaa aagcctttca aagtgatccg atatccgctt
     6121 atggtggaat tattgctttc aatgggactc tggatagcga tacggccaaa gcaatattag
     6181 aaaagcaatt tgttgaagtg attattgcgc ctgatgccaa tgaagaagca aaaaaaattc
     6241 tggcagccaa agagaacatt cgtgttcttc ttaccggttt ttggcaacaa ggtaccaatt
     6301 ttagattaaa tatgaagaaa gtcgatggtg gtctattagt acaagaacac gattctcttt
     6361 ctctggaatc atgtgagtta caaaccgtta cccaagcaaa acccactgat gaacaactac
     6421 aaaatttaat gtttgcttgg ttagcagcca agcatgtcaa atccaatgcc attgtatacg
     6481 ctaatgattt ggccactata gggattggcg gaggacaaac cagtcgagta atgagtgctc
     6541 gtattggttt gtggcaagca gagcaaatgg gttttgatcc ccaaggcgct gttatggcct
     6601 cagacgcatt tatccctttc cctgatacta ttgaaatagc tgcgaaagct ggtatatccg
     6661 caattattca accagggggt tctatcagag atgaaaaaat catttcttgc gccgaccaac
     6721 acaacatagc gatgattttt acaggagtgc gacattttaa acattaatgt caagcatgta
     6781 ttaattccaa gcttgactaa cttgaacaaa gagtcgtata taacgtatca tcttcttttt
     6841 aattgaagct ttttggttag cataaatgga ccttttcttg taggactgat ggacttgata
     6901 ttagaacaag gagcaagtat ttctagaagc agattttacc tatagaataa ataataatta
     6961 taggaatact tactaatcaa ctggcaatca cgcaaaagac aaaaatgcta ctgaaagtct
     7021 aatgcgtcac tgaaacccat ggagtaatat aatgacaact gagcaatacc cgtcctcact
     7081 tgtcaatcgt cttgcaatca cagagcctgc gttggttcca agtggatatt atcgttcgaa
     7141 attatttatt gccccatttt ctacaaatgt tttagttgca gcggccggac caatactgtc
     7201 tttattggaa agactttgtt taagtccttc tttacctcca gtagaggata taagagaaaa
     7261 tatacagcat gaattacgcg cctttcacag taaattagac gcgtctaaat atccgttgga
     7321 tattattagc attgctcaat atttattatc agccaccatt gatgaaatat taggtaaaag
     7381 ttatctaagg gtctataacc tgacgacaga gttcaaatca ttcactccct tatcaagtga
     7441 tggggcacaa ccccaacagc gcttttttga aatattgaat tacattaaag agaggccaaa
     7501 tcaatttctc gatttaattg aattagccta tttttgcctc attgccggat ttgagggtga
     7561 gtaccatctg aaagcagacg ggcgacaatg tcttgataat actattgaag atttatatca
     7621 aatcatacaa aaataccgtt ttaataaacc acatcgcttg tttaatgaga atcctcttcc
     7681 caaaactatt aaaaaaaatt ataaagcaac gtggataaca actattgctg ctgtaggtat
     7741 tgtaattcta gcttttgtta ctagccatgt tttattagaa aacaaagcga aaactgtttt
     7801 atttggacat acccaacttg ctatgctgga aaactaatgg acaattcatt acgcgcatta
     7861 tgtgatgcta taaaaaaaat tctctctcaa ttaaaaccac aagctaatca attatctttt
     7921 attgtcatca ccggaaaaaa cgcccaaggc aaatcggcac tgttaaagca aagtaatatg
     7981 gaagaaatgc ccgtattcag cgaagaacat gccaaactgt attttaatca aaagggaatc
     8041 atagtagagc ttggtgaaaa ttggcttacc aatagcaaaa cattattgct cacaacactt
     8101 aaacaattaa ataaatgcaa tcgtcatctc aaaattacag gtttgatttt atgcgttgat
     8161 gttaatgatt tactgatcgc cgaaccagca caattcactg aacaaaaaaa atctcatttg
     8221 caacttattc aacgtttagg tgttaacctg ggatatcagg ctgaattagc tataatattc
     8281 accaaaatgg ataccttggc tggcttttct gaattttatc aaatggatca tgtcaatgat
     8341 ctatccaaac ctcttggctt ttcccttgat tgtgtcaacc aatcaagtaa aaaaattgaa
     8401 acgttttccc ttcaattcaa tcaattaatt gaagctttgg gacagcaagt tatcaataaa
     8461 atgcatccgg tacgttccac tattaagcgc agtttgatac gcgaatttcc tttgcaatta
     8521 gccagcttga gagccccaat tcagtcgtta attcagggga tttcgccaaa attattccat
     8581 gttcactcta tttttttcac gagtgccgag caaggtggag tcagtgtaga taggctaaac
     8641 aaaaaaattc agcatgaata tgctttggtt gttcaggata ctttccctca agcgaccaat
     8701 ttcagatcct attttgtaga aggtgcactg aaaacaatcc aggaacaatg tatccaggta
     8761 ccacagacta caaaattttc tcaaaaacca ttcattgctg ttgcagtaag tttggccggg
     8821 ctcagtttat taacgctgat ttataaccac tacaaaaccg ctcatattct tgatgaggct
     8881 agtaaagaat tgcttgccta tgaagcactg aataatcagg gcactagcgg tgcacaagcg
     8941 ctatatcact tatcgcaggc tgcaaataaa atgaatcaca ttacatcaaa ctccatatca
     9001 ctgcctactg tgcaccaatt aaaattaaac cttcacaaca atgctcaaaa tcgtttacaa
     9061 ggagaatttc ttccttcctt agccaatgag ttagaacaag tcatcacaag tcctggcaac
     9121 acccctatca cccgttataa ggctttaaaa atttatttaa tgttaggaga ctcaaaatat
     9181 ttttcatccc aacccattct ggattggttt caacaacaat ggcagggacg tgcaccaggc
     9241 actacacaaa aacagttggc tcttttaaaa caaactctta gtaatcctcc tcaaaatata
     9301 cccatcaaat cacaaataat cagtgatgcg aggaattatc ttaatgcact tccaacaagc
     9361 tatttatatt actctattgc taaagaatat ttccctcagg aaaaacaaaa gattgtgatc
     9421 gaaggttttt ctatacccac ggatgaagta ccaacctatt ttactaaaac cggatttacg
     9481 aaaattatta acgaattacc taaaattacc agcacacttc aatcagaaaa ctgggtactt
     9541 gcgagacagg atttgtctca attacacaat atgctgattc aggcttattg ttttgattac
     9601 gtcacttggt ggcagacgtt catgaaaaaa tcccaaccat tacgttatca ggattatcag
     9661 caaggtcgtc aattaacaaa attaatacaa caatccaatt cgttttataa attcgctaat
     9721 cttattcagc aggaaaccaa accggattta actgaaggca tatccacctt taatcaattc
     9781 attgccaatc aatttactga tttaaattta acaactttat ccagcatcaa ggaattagat
     9841 tttagaattt ctgaactaga acgatttatt gcaactttat ctgttgtaaa tgatggaggt
     9901 aaaacagcgt ttaccattac acgctcgcgt tttctcaatg aaaattcctc tgaccctgtg
     9961 agtttgctct acagccaggc acgacaatta cctgaaccat tgagttcgtg gacaaagcaa
    10021 atagctggtg acatttgggt tatactgatt agagatagtc gtcaatacat taatagtcaa
    10081 tggcaaaaaa ccgtctatag agaattccaa aactctattg cccagcgcta tccttttgac
    10141 tcgtcacaaa ctgcagaaat agatattgca gattttaatc gctttttctc aacacatggc
    10201 ttattgaaca cctactctga acaatacatt aagccatttc ttgacacttc ctctccagaa
    10261 tggaagccca aagctgtgaa tgatttcatc ttgcctattt caaatgaagt aattgatgag
    10321 ttaattcgtg ccaatatcat tactaatatg ttctttcctg atcacagtga tgaaagcaaa
    10381 attgatttta gcttgcaaaa aatctctctg gatcctgtag tttctaatct agtccttgaa
    10441 attggcggca ctaaattgat ggataatcaa acaagcgact cactaattcg cttcacctgg
    10501 cctcaaaaca acgctaaatt ggctcttgat tctattgaag gcaatcatta cgagctcgca
    10561 gaatatggaa cctgggcaat atttaaatta cttgaaaaag ttaatgtgct tgtggatgaa
    10621 caagatagtt ccagcctcca aatacttttt gaagtgaata gcaactccgg ccgctattta
    10681 ttaaaaacaa acaaccaaat taatcctttt actccaggaa ttctgaatgg gtttacttta
    10741 aacgaccata ttgcataatt atgaatcaaa acaccaaaca cagcattatt gctggattat
    10801 acggaaatgc tttggagtgg tatgatttct tgctctatgc cagttttgct cctgtctttg
    10861 ctgaaatttt ttttccttca aaaatccatt ttgtttctct tatagcaaca tttagcttat
    10921 ttgctatagg attcattatg cgcccaattg gtgggctttt gcttgggcac tatgctgatc
    10981 atgtaggacg gcggaaagca ttaatactat ctatgtcagt aatgaccatt tctaccgcat
    11041 taattgcggt attacctgat tttaattcgt ggggaattat cgccccactt ttattttcta
    11101 ttttacggct cattcagggt cttgcagtgg gaggcgaact gcctggctcg accacctatt
    11161 tgattgagca tatgtttcaa caaagccggg ggctcgctgg aagcctcgtg ttaagtagcg
    11221 cttttttagg tatttttatt ggagcgctta ccgcatcaat attcagcaca ttatttacag
    11281 gtgatgctct attaaattgg gggtggaggt atgcgtatct gttaggaggt attttaggaa
    11341 ttttgggcat ttatttgcgt attaaaagct ttgaatcacc cacattccta aaggataagc
    11401 atactgctga acttcctgca aaagtcgtat tcacgcgatt taaacagcaa ctgtttttat
    11461 ctgtgataat tactagcatt cttgccatgt ctaattatgt acttatcgcc tatgtcacaa
    11521 cttttttagt caaatcagag tattttctct tgaaagatgc gttattaatc aacttaattg
    11581 cccttgctct tttgacagtt ttaataccgc tgatgggatt tctttctgat gttgtgggac
    11641 gcaagcctat tttcttaatc ggtttatttt ctctttgcat attaatcttc ccattttttt
    11701 ggctcttatt aagtggtagt tggtggaaag cattatgcag cgaattatta ctagcgatag
    11761 ctttagcccc aatcaatgcg acagtaccca ctattattgc agaaatgttt cccaccagtg
    11821 tacgggcgag tgggatttcc atatcctata acattggcca ggctttgttt ggcggtacca
    11881 tgcctttggt tgcgtttacc ctgattgaat taagcagcaa taaaatggct ccggcttggt
    11941 acctgttcct atggatcctt atcgtgatac caataactat ttccattcgt gaaagctatc
    12001 aaaaagaact tacttaaaac attagattga gataaatgga cataatttct atcgattgaa
    12061 taagcaacaa tagatttaaa tcagtttttt agcagaaaaa atccagcatt aatagccaga
    12121 aaataagtgc gttgagccat actaataaaa atcttacctg cttcaaccta cttttgcagg
    12181 caagctttta acgttgggat cttttgaata agcatcaaga atatcattca ccaattggtt
    12241 aatcattccc tctctctcat cgtcttcaat taacccaact ttttgcttca tgcttgctaa
    12301 tcgagcctcc aattctttag tcactgtacc atttgggaac agtgcagcta aaagccgtcg
    12361 agcttcactt ggtcccagat gcctatacaa aagatttcgt actgtggcat cttctgcagt
    12421 agtactaaat gcccataatt ccaccggccc caatgttaag gttaataatt gtacgttgat
    12481 acctgtttta gtagcaaact gagcaaggaa agtagcgcca ccttgtctag gaccatgaac
    12541 tcgagtacgt aacgcaatct tggctgtttc agaaagtcca aaaatttgac tggtcttctc
    12601 gacagcttga gatggtcctg catccataac ataaatagcg gtagcaaaat caatcattac
    12661 cggatcaaag tcatctactg cttgtgataa taaggaaatt tgcactttcc attttcggcc
    12721 ttcacgcata tcaataataa cctgctcacg aacagcagac gattttgccg tgcgatggaa
    12781 ttcatcataa acaatacgct tatgatcttc tcgaatctct aatactctct gtttgtgata
    12841 ctctttatat tgttccggta cattattcaa actttcttct gttaaataat agtgccttgc
    12901 caatacatat ctggctaaca tatacataac cgctgtttgt ctatctgccg catcaccacc
    12961 acttttcgct acttcatcca aatcaagaga cactactctt gcgtcaccaa tatcaaaact
    13021 agtgacccgt gataaaatag gatattcgcg aacagccgat gaaatcattc ttgaaaaagc
    13081 attaattagt gattcgccag ttggagctgt aatcttttca tacaaatcct caattgatgg
    13141 tgttctacag atagaagcag catccgctaa taacggcatt gcatatcttt gcgccaacat
    13201 cgcttcatgg acaaaacctg ctgaatagag cgagtcagtc acttcccacc aggttgattt
    13261 ggcatctcgg acaaaaccta tttcctccag gatgctatcg ataaattcct ctattcctgg
    13321 agcataagga gtcggattga attcatcagc catgctttta tataattcat ctaccaccat
    13381 acccgccaaa tcggccatac cgtcatatgg ctttgcagca cctaatggcg tagttagcaa
    13441 ggtcatgaag ttgactaaga atgatcgttc caaagcagtt ggataacgac atcccaattg
    13501 ggtatcaaag ggattaatgg aataatctgg ggtcatcctt aatctatgat aagcaaccaa
    13561 atgacgtttg gatgcaggaa gagcctcttt tagcaatgaa atcaaaccac tacttgaggg
    13621 acctatatca ataattgcaa tgcgcggcaa acgcattaaa ccacctgaaa gacaaagagc
    13681 taaattcagt gcatttgaca acacagattt accagaacca ggacgagcgt aaaccagatc
    13741 aatccaggta gtttgttcag ttgagcctgg ttgaaatggc cacggtttac catctggtgt
    13801 tctaaaaagc aaggctcctg ttttccaggg agatgctggt cttgtaatag gcagcattga
    13861 gataacgtct gacaaaggag ctactgaggc tactgctgag ctgttcaaag tagtagctag
    13921 catacttgac acaaagcctg caaatggatc cccacaaatc tctgatacat cagtagaacc
    13981 ccaaccttca atagccttta ctaactcaga acttcttcgc cgcaacaagg gaatattgcc
    14041 ttcaggggcc caagttgttg caacgacacg aagcctgaca atcgcctcat ctgtattgag
    14101 ttgtatatat ttcaacagat tgaccgagtc actaattaaa cgattttgtg cagaactaaa
    14161 gctcaatata gcagccaaga gcccctttaa tttaattgtg cttaatcctt cgctttctag
    14221 caaaaaagaa attttccagg gcacatgtga aggaagaata cgggaaaata aactaataaa
    14281 tggtcttacg tcctttggaa acaaatcaat ataggttgaa gaataaattt tatcccctac
    14341 tcggacagtg cgtaaatcaa taatctctgc atctcttggc aaaacctgct tggacaacgg
    14401 cggccataat aaatcagaag gatctccctc aaaattattc agttcgcgaa caggtatttt
    14461 gtctccaggt aaagttgccc gccaatcatc tgcagtataa tcaggatcac ctgtcatgcg
    14521 aatggcatgt atagcatcat gaacctcaag caacttagct atcacattaa gggcatccaa
    14581 atcatttaga attgcacgca cataagcatc atgtgtatct cttatttccg gaacagccgc
    14641 aaaaatcgtt tgtgtgtttt taaagggagg agcctttgta tccttgatca tcttcatttt
    14701 tgctttgttc gcagctttta actgatcgct cgctaaatta aatggtctcg tatacaaaac
    14761 aaaataaaca cgctcctctg cacaatattg tgataaataa ttgaccctct cctcaaataa
    14821 atctcttaaa ttaagttcaa gacgacttgc tgttgcttcc gcagggtcat aaatatcctt
    14881 gatcattttt actatgtttt gcttatcatg gctaaaataa acttgtagcg catgaccagg
    14941 tctacccata gcagcttgaa atgagttggc taaaccttca acgagtcgtt caaactcttc
    15001 agggcccgcc aatccagtaa tcccttcaat ttttaatact gacagtaatg agccatcatg
    15061 attaactaat acggtagggc tatctgccgt ttccaaatct atataagact ctgtagtttg
    15121 cttcagggaa gtacttaacc aggcaaagaa agtatcgacg ccttcaaaaa acgattctga
    15181 ccaatttgcc atcctttgtc ctcaattatt aatagtactt ttactgttta atctgcaatt
    15241 tcttctaaag cactggcagc ccatttctct atttgcttcc tgaattttgc tctggagggt
    15301 aatagcctta tatttttaaa aagtttctct cttatttctt cactattcac accagaatca
    15361 atcattaatt gcccaaatat tgcagggtca cttgtgcctt caccaaattt ttcttcgaca
    15421 atttgcgagc gaaatttaaa gcttctataa atattctgtg cactggattt gtatccggta
    15481 tcattggtaa tagcacaaaa aagaacatgg cataagctat ttagttcagc ttgggttaat
    15541 tctggcaggt agatcaaagt acctccacca taaccaccaa ctcctactga ctcgacaaaa
    15601 aagcattgcg cacaaaaaca acaagctgta actaaatttg ataacctatt gtttgtataa
    15661 tcaccatcca gattcacgat gtcttggtat aatcgggcct gaaacccgca aaattgacaa
    15721 gtatacctat ctcgctgaaa gattttttgc tcataggatt tgaatcgctc atcaattttt
    15781 ctggcagaat ataaacgcca ggaacctggt gaagcaatta attttagctc acatcgttgc
    15841 tgattatccg ccatattttt ggttcacatt tagtcaattt tcaaaaaatt atgtgttgta
    15901 tctttaacta ctgactgcgg tcagcttaag aatactggac catattaata gagacaatca
    15961 tttaaactta ctatttaaca caataaaaag tctataaatt aaattgggta aagctttatt
    16021 ctctttaccc aaataatata aataatctct ctattaatta gtcttatata ttgttccagt
    16081 aggtccagca gttttacctc cactactacc gaacatggtt acaccggcca ctcctaaaat
    16141 agaaggaagg aataataatg ccgcagcgat aaatatcaaa gctattggtg ttccaattgg
    16201 aatttgagta ggattatcct tgtgctgctt aaatttcata atagcaccaa tagaaaaacc
    16261 caatccagcc aagtaagagc ccgctgtaat caatttagta agacttgtaa atgaacccgt
    16321 aatttgtgat gccattttac ctaacgatac acctgctgca gcatcctgac aaattagtgt
    16381 caataaagcc aaacaagctg cacttactag ccagtgttta taacctgatt tatggataac
    16441 cttaatgttc acaattctct cctgttttta aattagctta agtaccatat aaagtattgt
    16501 taatcatatc aactgtacca acaatattga ttgccaatat tcccccaaat acatgaatca
    16561 aacctttacc tgtacctcct ggaggttgcc cctgcgatgc ggaacgcgca attaataccc
    16621 accctcttac aaatgcaatt agaccaataa cccggataat catcaccaaa ggtctgccta
    16681 ccagactgcc actaccaaat aaggctgaga tagtatcgct tccactactc acacctcctg
    16741 agtaagccag cggattgcta taaccaaaag tcgtttgcag tacggaggaa accaatgatg
    16801 ggaaatagat aagtaatgca cctaccatca aatacatcac cggctctttc atactcgtat
    16861 tgctcgacat cattgtcctg gcttctccat aaacctttaa actataaatt gctttaaata
    16921 taaaagcaca cccaatcaaa taagcgcctc cagtaatcag cctttgaaca ggttccaact
    16981 gattagctat attagctaat atgttagctt gactcactat ccagcatgct actgtgccgc
    17041 cagtacaagt cggatcagcc atatacgcct caactatctt cttgactaaa cttaatcact
    17101 tgtccagaac ttgttaaaat acgaccttgc aaagaatcaa ttaatttaac cattccatat
    17161 cctggaattt ttgatccctc tctaactgta agtgtagaac catttgagcc aattagccaa
    17221 gcccgacctg gaattactgc ttgtatgtaa taaataattc ttacaggaac tgttggccga
    17281 gatactttta caactttctt aggagttgta cgcgccatta agacattaat cacttctgat
    17341 tgtctagcta tttgatttga catattacca ataatttgat tcagatttac aatttgagca
    17401 tttaaatttt tgatattgtt atttactgca ttgatttgtt caccaagcgc attaacctcc
    17461 gaacgaagac tctgttgtgt cagttctatt gctgaaactt tcttcttcag atcgggatca
    17521 tcttcaataa tagtcgtagg ctgaacctgt tgtatgggag tggtagttgg tattgtctgt
    17581 acaggctgtg gtgttgctac ctgtgtcact ggaggaatag cgggctttgt ggttacttgg
    17641 ctggatttat ctgagaaaaa catccaacca attattttat acatcaccat tataaataca
    17701 actgccccaa tagcaataag agcgtttcgc ttaatatctt ttttctttgt taatccctct
    17761 ttcccagatt gaaacgaagc ttctgaatcg aggtccgatt caccagcctg atccatatcg
    17821 tatgagtcaa gttctgcaaa tttatattca tcattatttt gatcgtgctc tgccatcatt
    17881 atccgccatc aaattaaatt gttgtaacat cctgggtaaa taaaataccc aaacctgtac
    17941 cagaataaac ttccacagtt gttggtgtat taaacaattg ttgcgcctgt tgactccatg
    18001 cctttccaac ggtagccaat cctataaccg cgttttccaa agttgagcga ccaacaccat
    18061 tggctacagt aatattatta ccaccaccag tacccccaat ggtgattgtt gtatttgccg
    18121 actggaatgc atttccaaat ccttgtagga aagaagaagc aaatagggaa ccatatcgca
    18181 ttaaatagtg atgattagtt ctactggata gggctgttct tgccgtgttt ggatcaattg
    18241 cataagccga gatggatata gttttttctg ctcctggaat tgacattgta ttaaaggtaa
    18301 tcaccatctt gtccgcattg gacggtaagt tgaagcttcc tattaatttt gagcctttta
    18361 gcttaccagt aacaatagtt gccaatattg gacctggctc atcactattc acagcggtat
    18421 ccagaaccgc aaacataata tcgcccgttt tgataatagc ctgattctga gcaccacttg
    18481 cgccttgctc tactggttga ttattagcac ctgcaccagt acctgctcct ggaactgagc
    18541 tttcaccacc agatgttttt gtttcttctg taccctctgt atacacctgg gtttcaacct
    18601 tcttccagtc ttgaaccagc tgagtcgccg ctgagagcat gtcagaagtt ctttgttgaa
    18661 tttcttgctg atatttttgc tcagccaatt gttcattttg cttttgcaaa atggcctgaa
    18721 gttgctcagc acttgaggca gcatcactgg ttgttggtct tggagtagcg ccaggaattg
    18781 aaggaattcc tgtaatactt gagtcgagtt gttgcaaatc aggcccttgt aaccctgaaa
    18841 cagctgattc ttgtggagaa aatccagcat ttctcagctc agctgaggta tatccggctt
    18901 tttttagctc atcagccgaa tatccagcat tctttaattc tgaggcacta aatcctgcgt
    18961 ttttcaaatc tgatgcgcta aaacctgcat ctttcaattc ttttgcgcta aacccggcat
    19021 ttttcaaatc cgaggcgcta aaaccggcat ccttcaactc ctttgcacta aatccggctg
    19081 cttttaattc cgctgcagag aaaccagcat cttttagatc ttttgcagaa aacccagcag
    19141 cctttagctg ggccgcagtg aatccagccg cttttaattc agcagcagaa aacccagcgt
    19201 cttttaaatc ttttgcacta aaacccacat cttttaattc ctgggcggag aaacccgcat
    19261 cacgcaattc ctttgctgta aaaccggcat cttttaaatc tttcgcacta aaaccagctg
    19321 cttttaattc agctgcagta aacccggcgt ctttcagctc ttttgcagta taaccagctg
    19381 ctttcaatgc cgcagcggaa catcctaatg tttgctttat tgtcaaggca ctaacaccag
    19441 ctgccctcgc tttttttaat gactctacgc tacagtctgc aacacggcca tctgcaataa
    19501 tggatgcagg atttaatcca gcatcttcca actgctttgg tgtaaatcct gccgcaagca
    19561 attcttgagg tgtaaatcct gccgcaagca gggattgcgc atcatacccc gcagctttta
    19621 aagcttccgc actgcaacca ttgagttctt tgatgcgttt tgcagataca cccgcagaaa
    19681 ataatttttt cagtttatca ggatcacagc cagcggcctt tatttccgca tcactcaaag
    19741 gagttgctgc actgatttga gctggtgtaa atcctgcgct cgccaaatcc gcatcagtga
    19801 atccggcagc tttgagggcc tgtgcgctac atcccgcgta ttgtctgatt aaagcagcac
    19861 taactcctgc ttccctttcc tttttcaaag cctccacatc acaaccagca ttcttcacat
    19921 cttgcggagt aattccaggc ggaagctctg cctgagcagc tttgatttga gcattagaaa
    19981 atcctgcctt tgccagatca gctggtgtaa atcctgcgtt taacaaatct cttgcagtaa
    20041 atccggcatc ttttaattct gcagcagaaa aacctgctcg ttttaagtcc gcagcactaa
    20101 aacctgcatc ttttaactct ttagcggtat aacctgccgc tttcaactgc tcagcggtgc
    20161 aaccacttat ttttctaatc gcggacgcac taacccctgc ctgacgtaat ttggccaatg
    20221 cagcagctcc acatcctgct ttgcgcacat catctgcagt tataccatct ggtaagccac
    20281 tcgcttttgc aatttcagca tcagagaacc cagctccttt taattcgccg tctgaaaaac
    20341 ctccatcttt catttcttga gctgtaaaac ccgcattacg taattcacac gcatcaaacc
    20401 cacaatttcg cagtcgacct gcactataac ctgcttcttt caactgcctt gcattataac
    20461 cagcggcttt tagctcttta cactcacaga cctgctctaa ttcttgcaaa ccatatccac
    20521 tatcttttaa ctgaacacag ctacaagccg ctttcaaatc agttaattga gctccctgat
    20581 tcacaacttt cgtaatcaca gatttgctac aaccaccatc ttgtaactct tgtatccata
    20641 aacttcgttg aggaccacct aactcttctt gagccaaagc agcaaaaccc actccacttt
    20701 gggaaccaat aacaccaact ccttctccta atgcttgggt gcggataatt gttggaatag
    20761 cactcccacc tgttttttca gcgacttgtg cctgggttat attttgttct tcttgcaatt
    20821 tggcatattg agcggttgga tctaaaactc ctggaataga ttgaattcct cccggcactg
    20881 tagaaacttc agctgcagca atcgaactgg tggtagcgct tcttatttta aaaaaaccta
    20941 ttactaccgc gatgattaat aaagctgctg taaaaatgat aatcactcga gtccgagtat
    21001 tggaaaacag tgactttaga ttttcttttt tgctcgccat ttgcttataa cccttctacc
    21061 ttgagttgca tgacttttcc atgccaagac actaataaaa ccggagattt ttgcatttca
    21121 taagcatgcg ttccatctgc gctagtcata ctggctaacc agcccggcga caatatagtg
    21181 agattcgttc taacatacat tttttcatta gataaccaag ctcgagcatc cccaccacta
    21241 accaccagac gcctgcttcc tggcggagga acaccttcta acacatgaag aagtaaatca
    21301 ttcgctgatg ggggaatgcc ttcttcagtt ggcatacttt tagcgttggg gccatagcct
    21361 tgaactctca agtctactcg ataatccacg gctttctgcc cgggtataag agtaagcatt
    21421 acaggggtat ttaatcctct taaccttact gccaaattac catagttata tagcttcgta
    21481 gcttgaatca ttaaagtatt actggtttta tcccattgaa tattaaacga agagggatca
    21541 cccaaatcat atgcagcaat aggccaaggg gctcctgttg aatccaggaa cactaaggaa
    21601 gacacaaaac cttgtgataa tcttattacc ggcggtgttg agcctggaga cagattaaca
    21661 aattgtgatg tcgctgtagg tttaggtgga gtgcctgccg ttgaagcttt ggcatactcg
    21721 gaagtctcat aaatttgttt taatttgact acttgctcag gatttaaggg atataagttg
    21781 cgtgtcatat ccttaaatgc cttcttatca ataacttcgg catcatcttg actaattatc
    21841 tgcccatcac cgcccacatt tgctgtttga tttgcagccg gagcattagc ctgcccagat
    21901 tgattaggtt gttgcgttga atcagaagct gcattatcac ctccatcacc tgctcctaac
    21961 tgtacatcag gggaagggtt ttgtgataat ttctgctgta gcatacgcag ttgctgcaac
    22021 gcttgctgag catcatctga ttgatcagcc gcatagatac tgcacgacat actgaatgtc
    22081 aaagcaatca ccaaacaata tttacataat tgatcatatt tcttcatcac taggataccc
    22141 caccgctggc aggtccaaca acaaattgtg aaatacctat ccctctaggc gaattcaacg
    22201 ttgaaactct agttattaac attgttacta cgttattttg ttgtgtaaat tcactggcac
    22261 tttgataggt gactaatatt ggcatttgta ccctccagga atacctacca ttgagcaccc
    22321 ctttttgcaa aatgattgga gctcgggttg ctactgctga cacaactaat tttttcgctt
    22381 taaccgcatc aaggttattg gattgctcta atgcacctaa aaattggtcc cagccttcgg
    22441 cagtaaaaaa gccggaagaa gcctgtagct catcgcgata atttacgaaa ttataggtaa
    22501 atgcggcaat tgctgcctga ttagcccatt gtagtacagc ggagtccgat tggtttggtt
    22561 catccaaagg aaataaaggg gtaatacgac cgttaatact tgttgcaaag tactttggag
    22621 caggtggatg agtaatcata tagacaacaa gcgatgccaa gaccacattt accacaatag
    22681 caatcaataa cgccaaaatg actttgcgtt gactgtcttt ataaaactta tttcttaatg
    22741 caactacagt taaggcatct tcagccataa tatcctctaa aaattattgc ggtataatcc
    22801 tttgtgaatc ttcatctggt ggatccggcg gaagcaatgc ttcagatgac atgttaggag
    22861 tcgacattgg cttaagctgg ataggaggag ttatgccgct ggtcgcataa aaatctcgct
    22921 caggttcatg tatatgaaca taataaatca acccagacaa accacaactc aatccaagag
    22981 aaagaattag tagtgttaag ccacgtctgt aaatatggac attaaaattc ttatttcgtt
    23041 ttattaaagc ccatgtttct cgactcattt ttacctcaat tatgccacag cccgcctaaa
    23101 tgtgatctcc accctggcat taggtgatct atcaccacca agagtataac tagttatagg
    23161 tttatcacta cctaaccctt gtgtaaagat gattctgcta tcaaccccct gagaccatag
    23221 gtattcactt actactcttg atctcgccaa tgtcaatgct cgttctcttt ttaccgacac
    23281 atatttacta ctgtaactgg ttaccgttat agcaatttta cgaaattgtt taagaaacgc
    23341 cgcgatctca tttaaaaggc tataggaagc ccaatttaac cgaggagatt gatcagcaaa
    23401 cagggcgcta gcaggaatac taattaaata attttggcct actgatgcaa ctttaatccc
    23461 ttttttattt aaatcagtca tcatttttat tattgttgca tcgcatgcac catcaacacg
    23521 gcatggtaat ttgggcactt cattctcagg gatataacca gaacgattac aagcactcag
    23581 caacaataag aataataatc cagttgtttt aaacaataca tagatgtagt tagtacgaag
    23641 tgatctcacc tgttatcccc atctttgaaa atagactaat tgtgttatct atgtctaact
    23701 atctcaaaca ttccatttga taataaaaac atacaaatca ttacgttaca atgaatttaa
    23761 taaaattgcc atttttttaa ataatttatt tatttaagat ggggttgact aattaaggca
    23821 aatcaacata tgccaaatta atctatgagt aaactgataa ttaccctgtt aattcagaat
    23881 acaaaaagtt ctccccaaga ggaaccacac agaatatcaa cctgatagat caaggtttaa
    23941 agaaacaacg tcactgcccg gattataaaa aactcagagt aacttacata tggagctctc
    24001 ttcataccca atagcacagg aacactgcta cctccaattg ccgactgagt ctttatgtta
    24061 attcctctgc agctttttta ttggctttct cacgttctgc ggaaatttta gctgataaat
    24121 ctcttataat accagtaagt tcttggacat caatcacatc cctttcttca ggaggatagc
    24181 tagtcgctat ttgaaaatct ttgattaatt cattagctac tgtaccagcg tatttgtctt
    24241 tagcccctgc caaacactca atcgttatca tttgatttcg tgtttcatta atgggtaata
    24301 atggctcaga gaaaacttct ttgtcagcga ccaatattgg aggagcatta gggtcaattc
    24361 gtagtttgct gaaaatagtt aatgccccct caacttcttc ctctacaata tcctccacag
    24421 gctcgggttc attttgacca tgaaatgcaa ttaaagcagc aacgcctcgt tcaattggct
    24481 ctacgatagt agattctttt aatgccttac taatgagagt gatttcttcg ttctcgactg
    24541 cattattgat actcaagtca ccgctttcaa gaatgctctg gaaggaagcc aattgtttct
    24601 gtagcttcat taaataatca tctggcggtg gctctacttt taaaaattgg ttaattttca
    24661 attgtttaac cggtttggga ttggcataaa acatgcgagc acgaacaatt ttggatttga
    24721 aaaatatatg cgcctcacct tcggtttgct cttttaaatc aaggaggtct acccgagctc
    24781 tcttttcaaa agaagagctt ttagtatcca tatagctgtt ggctatgctg gtttctttgg
    24841 tttgaaacga atccactttt gtaacataag cttcgccagc tgttttagtg aaaaaatccc
    24901 aggtttcggt aggatcttcc agtttcatac atattttaat gtttgtgttt gcaccaatgg
    24961 aagctgcctc ttctttggat gctttttgga atgcaggtaa gtcttgtcca gcaaaaatag
    25021 cagaaaatcc tagagaacga gcttgcgcag gcactaccgc aaatccttgt actgcgtaat
    25081 aaccatactc atcaagaatg cacatataag gagttggggc attcgtgggt tttcttagaa
    25141 taacatcacg ataatcacct tctacttctt cacctaaacc tgccgccatc atagctttta
    25201 atgatgaaac aataatcttg ccgaggttag ataattcatc cggtgatttt tccagagcgg
    25261 gtaataaaac cactaatata cgtctgttga gcaccacatc tttaaaatct acttctgcta
    25321 aattggtgcg gataatatga ccataagtat ctgccagaga agagaaactt cttaccagct
    25381 gcatggtaat aaagccatgc tgttccaaaa cttgtgatac ctgtttcccc tttttctctt
    25441 tattataccc aggcaaggta tttaaatagt ttcttaaagg atcagttacc agttttggaa
    25501 tggtttcaat attgacgctt tcttgatcat ctctgggaaa aactttatca attacaatag
    25561 attctagtct ttgcaaatca aaataatttc gaatagtatt cgcatcaaga agaattgccc
    25621 cttcatcacg catatacacc aataaacgca ttaatgcttc tacgaaagca atagcccgtc
    25681 ccttccacat atcaccatca gatgattggc ctgaagatcc catcaaactg actaccaact
    25741 gagttagcat actggaagaa ccttggcaga atgggtttag tgtattggaa agtctttttt
    25801 cctgcggacc gacaatatcg cgcgctccag tcatgaagtt gattaagagc aaatcatctt
    25861 cacgccccat gctccttacc atagaaaaga ctttagcata cagagagtta tctcctttac
    25921 catcaacgta gataaacccg ctcccctgta ccagggcatt atatgacaga gaaaccaaag
    25981 tttctgtttt accactaccc gttgaaccga aaattaacgc atgggtacgc atatcatcgt
    26041 tagcaaacca aagttcctcg ccggtctttc tgtcattacc aaaaaaggta atacctcgag
    26101 ctatattcgg tttattaata cccggtttca aatcgttgta atccttcgca cgcgaaattt
    26161 gaggcaatcg aaatggcaac ttttgctttc gggtatagct ataaagaaaa aatatgctcc
    26221 ccataatcaa caaaaatgtt gctgcttcag agaaataata tgaaacagct gctaatgaaa
    26281 taagaacaat agaaatattg gtcggatctg caaaaaaatc tgccaaccgc tgcgtaaaag
    26341 tacgcgtatc cctaagtaaa agggttggat ctaattcatg acgagaatca ataccccgca
    26401 tcatggctct aattcctcca tttgacgtgg tgttaatctc acctctttta ctgcaatttc
    26461 cagagctcta atcgcttcat ctatcattgg caccaaagag cgtcttccca tttccttttc
    26521 agctttccaa tgtgcgaaag gacctgcaac ttcagagtat ggagtttgcc tgcctacaca
    26581 attcaacata taccataaac gtctgtcgac tggtttgagc cataaaaact ctgagctggg
    26641 aaccacacca tcttctcgtg ctgcttcaag caaagaagca atcactgtga gcacataagc
    26701 atgtttggct actacttcct gaaccaactc tgagttttgg tattttttca ttactggcct
    26761 tgcaacagaa aaatcaggtt tcccatccac aaatgtttta tctaaaactt taaggatatt
    26821 atttgctgca tctctatctc tattcattct tgccataaat acggcagaca atgcgtaagc
    26881 ctgaggagag caacgttcaa aaccatccca ataaggaccc aattgcatgg taaatactcg
    26941 tttggcatcg ccacgccgaa tccccgctgt catctcttga ccaggcacag gattatccaa
    27001 taacgcgtcg tcctttctca ataaattata ttttcgcgca aattccatgg gtgttaaagc
    27061 cattgcccaa ggccccttat tgacgtcttg actaaccaaa tcctccttaa ctataggcat
    27121 gatggcgggc caattaaatt gttcctgagc ccgcaaactt ttcatatcat aggttttgcg
    27181 atattttaaa gtcacatttg agttataaag aacaaaggct aacactacca ggatacaaat
    27241 aaccgggtaa cgcatataat caccgaccgc acgcatcaca gtcaccattt gatcccaatt
    27301 gacagtgtta ggatcaagtg tttgcatcag ataaatttga ttggccaaga gttgattatt
    27361 taaaaacaaa ttaactaatc ttgcttgcca gatattgata gtaaatacaa aactaacaat
    27421 gtactgatga gccagtgccc aaacaaaata agcagtaata aataacagta tgacgatcca
    27481 tactggcgcc atggaattat ctgaacctga ttgctgctgt tgttgtgcca tctctatata
    27541 caattagtta atagattcta tatatcaagt atatactgct cagagctatt tttgcgagat
    27601 tttcgtgtac tatatcaaaa tatgaataca aggatgatta aataaagcgt aaagatagtt
    27661 agagtagaaa taaattttta acacactcga aatcattata aggaaaaccg aaacggccta
    27721 tgcattttta attagacggc cattggagta tcgatatacg ccactcacat gaacgaagcg
    27781 actgatgttt tccagactga tggatttatc tttaagcgtt aataacggtt tacctagttt
    27841 atctaccgga gccttatcag cggataagtg caagatatct gattgactga tgtaaattgc
    27901 cacatacgct tcattttgtt tattttcttt tgtttttaat atcgctttta tgtcttcttc
    27961 atctgcgtaa ataggtcgtg aaatcatttg tcggggcaag ctccccacta ttttctccca
    28021 ggactgaaga ttgcttccat ctgaggaata taatgaaaca tatatttcct gctgaccact
    28081 ccttaaggca attcgattgg caagatgaga ctctgctttt aacttggcct gactagcggc
    28141 acctatccgt tttaaaaaac gatctcgaat tgcaattaac tttttaccaa ttacccttaa
    28201 gaaattagat ttatcccatg gacctttttc aatagcatca tttaaggcct ttagaatggt
    28261 ttctttttgt tcatccgaga gttgatcttt cataacttca atacttatgg gaaccaagaa
    28321 ttaggggaaa tataaagaaa gtataacagc atttgtaaaa atcactgaaa tgaaattaat
    28381 ttttcaagat gataatttga aaccacgttc ctcatgactt ggttgctcag acgatttttc
    28441 agattcagta gcaccaaaca ttctttctgt ctttgctgtt aaaatcggtt gatttaaggc
    28501 ggctctctct acttttgtat tgagctctgt taacacatca actgcatccg tgttatcctt
    28561 ccctccggga ttaaagctaa cagcctttgc aacagtcaaa atacaatctt ttacaggtga
    28621 aataatttca ctatagacac tgccaagagt agcatcagtt acatcagatt cacctatctc
    28681 tttaacacga ggtggcgtat aaaaaccaaa tggatttcgt gcactgtcat cagtattatt
    28741 acccattatt accactcctg agctcaatct cttactttac atatatcaaa atatatcttt
    28801 caatatatcc tgatccgata aaactttcta attttttaca aacaactaat catacattaa
    28861 ctcatccagg ggggtataat cacggcttgg gcccccttga ataagctcat tcataacatc
    28921 cgtcatacat aacaagcgta atacattcgg cgtcacttca tcaaaaacca ctctaatacc
    28981 tagtaacgct ccttcagact cgtcataagt cgctcccaaa ccataaccca aacacaagga
    29041 acacaattga tccgctcgct tggcaatggc tggtagcaaa cccgtttctg aaaaaacttc
    29101 atcaaaactt acaaatctat cgttcaggta aaatttcgca ttcatgcttg ctgcaatctt
    29161 tgccatacac ttgctaatat ctcgctccat ttatctccac caaggtctag cagatctaac
    29221 tgaaccagct aaaaaacttc tgagccagcg taaaaaaacc gggacagtga aaccataatg
    29281 ttcaattatg ccaaaaaaaa ttgcagaaac taagaccaaa acacctgtcc atactcttat
    29341 atgcattaga aataaaaata ttgggaatgc ggccctcgca tcaactacaa aaaaccgcgc
    29401 gcttctcgct gagtcacgcc agtggctcgt tgctgaaaat ccacctgcca tttttaaacc
    29461 tcaaaaaatc atcctttaaa aaaagtatat acttaaaaaa cagcttgtgc acgttgatac
    29521 tttcaagaaa cttaaattaa gattaatttt tacgcaattg aatattgcaa acacgaaggg
    29581 gttaaccaca aatagatatc ctaagtgtta gtaacccgga cttaatccat tggagaatgt
    29641 tttttattag atgatatccc taacatatta gataaaatat tttgcatatc aggtttcgct
    29701 tctggttcag cttgaagttt taactgtgga acagtatccg cttcagcttg ctcagtttca
    29761 gcaattttat gactgcttag ctctttgttc ccttgcattt tagctaacat tttgtaggta
    29821 ctgtggatat ccatctgaaa tcccctttta acaactctta aataagatat cacatacaaa
    29881 ttaaggaatt attaagagtt tgaaaaaaaa tcaaaaaatt cggcactgac cgatcattcc
    29941 ccctttataa aatttaggtt tcttaatatt aagttaagta ttttacaata caatatcatt
    30001 ttgaaagcga ttatcaaatt aaaagttgcc atattaatga caaactgtaa aaccagtgac
    30061 actttgctat taaaacccat tctgggatat agttgtttta atttttctat aagtgtcttt
    30121 attttaattt tgttttatat cccgctgaca tcaggcacat gttgggcaaa aaaaagtgta
    30181 aatcacaatg aggttgtaat gagaggaaaa agagaatttt taaaacatga atactcccct
    30241 ggccattggt caattgatta tactcgggca ggcacctcaa tcgcagtgat cactgtccga
    30301 aataaatatc attactctgt gattcttaac cctaccgatt gtagaggtta tcgtatcatt
    30361 attcgatatc tgaatgaggg ggattcaagc ctttcatctg cttttaaccg cccttatact
    30421 gtatctgaac aacgcggatt gaatgacgtt gcaagtttga tgactcaagt ctatgaaaaa
    30481 ttagggctta ttgtgcagtt ttctcaactt ggcaataact cccagtcttt tgataaagag
    30541 acaggagtaa cgctcattgg aagtgaagaa gaacccagta tgcttcattt gcatatgtgg
    30601 ggcaggggcg atcctgacat ggaatatatc gccggtgttc ctcttagagg gcccgaacct
    30661 ggtttgatgt ttgatttaat tgcaaagaat aaaactcacc ctattaacca acatgccata
    30721 aaatggaatg aagatgagct caaagcctgt cttgccatgt tcaagcttaa actggctgaa
    30781 tacgttaatt caccagagtt tactgaagaa tttggcgata ccctaaaagt gactattcac
    30841 gacaaaaaag atcttggcga agcgaaggaa agtggttttg ggtttttcca taaaatgaaa
    30901 tctgcatcag atggtataac aactttggaa aaaaccttag ccctggaaga tgataaaata
    30961 aatcccttcg tataatcgac agagcaacat aaatggtatc gtctttattg tactcgaatt
    31021 tcagccagga aaagtgtcaa aatttccggg taaaaatgtt ttcagccagc gactattaac
    31081 caagcgcatc cactgtctac ctgttgctac taccaacttg atagtggata atcttatgaa
    31141 acacatatat gcagattttt tatctgttcg aattcaattt atcaacagct caattgatgg
    31201 tcatcaaaaa aactgatgaa tatgtttaaa gcactggtat acaccctacc agagtggcat
    31261 ttattggcat taagcctctg gcataacctg tttattttgt ttcaggacaa gggacaaacg
    31321 tctttaaata agcaccacag aaaagttcac aacgcggcag taaaataaaa ataacaggtt
    31381 atgcaaagca gtctattcgt ctcggcaaaa gagtgcatca acacatacaa tgaagtacaa
    31441 actataggtc tattatgaaa gccacggaac ctatggagaa gagaagttgc ttgtctttaa
    31501 aaaaatttaa aaacgagcta gacaaagaac aggacttacg aaaaactcca aggtcaaggc
    31561 aaaaaatgtt tttaatgagg gagtttagat agactaaatg accgaattaa aaacattttt
    31621 taacatagag attggggttt ttcgtaagtc ctgtagaaat tatatttgag tgcaaaaatc
    31681 aatcttgctc agggtgattt attcgaaaag atatattctt catgcaaaat ttccctgaat
    31741 ttgatgcctc ggacaagccg aggctagtag ggaatgggaa ctcaaattat cctttagcac
    31801 ccataaacag ttccatcttt tggcgtatgg tcatttttct tatctccact ctcttcgtct
    31861 ttcttgctga agaatgaatt tgatttgagt gcttccttaa catgcttctt attaacaaga
    31921 gcctgagtat acgtggttgc atcattcatc gcatgctgca ggaatccagc aagctcaggg
    31981 ttttctttaa aatagctata aaggttaact tgaacaccat atcctttgaa tatgtcttgt
    32041 acataaggat catgaagaac atcaatgacc tcaagcatgg catgaaaacc gcgcttggca
    32101 taaacaccta tgacctgaat aagaaaaaga ttgatatcgt ttggcagatc gggatcttcc
    32161 ttataatttt ccatatactt ggctaacata cccacaatat tgcaagtatg tccggaaatc
    32221 gatcctacgt aagacgaata ctgacggtta ttattccaaa accattgttt tccctctttg
    32281 ttatcaggag caacagttga agaatccact agcatattac gcatggggtc gcgttgggca
    32341 tcataaaaga caggcgaaca gcctttcatg actccaccgc cacaattcca tacatctgaa
    32401 cctacttttt ctgagtcagt ctcaaatttt ctttctttaa tgcccgtcaa tttatcccgc
    32461 tcaaccctat cgcgcccttt atgcgcttca gcgtgatgta atacaccggt aataaattca
    32521 acttctttct tggtaaattt agaggtatcc ttatagccat tggcaaagat acaaattttt
    32581 tgttccatct cccgcggagt atccactctt ccacaatcac ctaaaaagaa ttgcagaatt
    32641 ttattacatg ccttcaatgt gttatctctc aattcctcat cttcaggctc tttatcttct
    32701 cctatcgaat caatttcaat ggtcttttct gagtgggaaa gaccgttaat gtactgagca
    32761 aggcgagttt caaaaaaacg tcttgggtca ttactcgtgg gattaaatat ctgaaggctt
    32821 tctacagctt tcataatttt ttgaattttt tcttctccta attgttctat tacttctttt
    32881 tcctttagtt tttctattat tttatgcaag cagtcatcat atcgaacaaa taaataatca
    32941 gccgataatt cttgcaaaaa ccccttatct ccaattttta aggaagtaaa aagaccaatc
    33001 gttaatatta aaaactctga aatatctgtt ttgtcaccaa caagagacaa gtatttgctc
    33061 aagtttatgt tcgttttctg atagctggga tcaccaatcg atggacgaaa tgcgggtaat
    33121 acagaatcaa atgagatatc agttaattgt gacatgattg attttgcaat cgaagtatcc
    33181 gcgtttgcaa ttaaaagagg cagtaactct tttttagttt tcatgcttaa agtccctgtc
    33241 ttgctatcaa gagacagctc agggatagac tggaaagcat tcatcagaat agtgagcaat
    33301 actttctcac tttcattggc aagcatctct tgcacaaact ccagaagctc aggtgagagc
    33361 ggatgtgctt tgtcctgact aaaagcaccg agcatctcca gatcacttga gtaaggtatt
    33421 aattcaattt gtcgtataat ttcttcaagt agaggtttat tatgagtcaa tagccctttt
    33481 aagaaaagag attttaacaa tatatgctta gatttcggtg tttcctcaat tgcttgccaa
    33541 atctgcttaa atcgagaaaa gcgtatattt ttcataaaat catctaaaat tctttgggtg
    33601 tcaatagtgc tgactgtagt atctacggag agttttttgg ttacttcgtt aattttttga
    33661 accgtttcat cggatacctt aaccacatct tcagcgactt tccttgataa aacacttagc
    33721 ttattagctg tttccttcga agtctttttc agatcttctc ccagtttctt tagaatctca
    33781 tctaagtttt gcatataaac tctacctctt aacattgtct tgcctgacaa cttatatttc
    33841 acagggggta tgataaagca caaagattaa gaaaatatta aacttattgt tcaaaattaa
    33901 aaaaaagtgt cgtttattta aattgtcacc tgaatt
//
LOCUS       DATNSN010000017        32442 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.017, whole genome shotgun sequence.
ACCESSION   DATNSN010000017 DATNSN010000000
VERSION     DATNSN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32442
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.017"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            24..953
                     /locus_tag="VAH76_000654"
     CDS             24..953
                     /locus_tag="VAH76_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO1385302.1"
                     /translation="MKLLASLCAFLLSGVVSATPLNNIVVFGDSLSDNGNLYEYMKHQ
                     LPQSPPYFEGRFSNGPVWIERLAASYFPNDPNSHLLDYAFGGAGVSVDEEDDEVFFTL
                     RREVNSYLLAHQDKASPDSLFVIWIGANNYLGMPVEVEETLKNVNRGIADSIQRLVDK
                     GAKHILVLNLPDLGRTPAALEFGSVEEMTYFSAQHNNALSNTVDYFKKTYPEVEWLFF
                     DTGSHFDHVIEHASEYGFTNITGTCSFSIVDEITKNSVLKMVASVKPELTESACDGYL
                     FFDLVHPTALAHKIMAEKARLMLDEAGVEFAEN"
     gene            1145..1918
                     /locus_tag="VAH76_000655"
     CDS             1145..1918
                     /locus_tag="VAH76_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HEO1385303.1"
                     /translation="MNQIKWIVLLFCFVVSNVFADKLDITVDQKKIILPYWLSRSVSY
                     GAVIIVSGGETAQWSLLLEQFAKGLSRNGWSVVLLNCTKNNSIPWINQLPEVISTLRQ
                     NNNKRIVLVHYGDQLNLALEYFSKPQSKMINGLVMLSAYDLNRNLQKVPRLRFPLFDI
                     VGQFDYDMVRQQRKSREEKFKENSYLAIDIPGATHDYQYSQQLLLAFVHGWMAKLPEF
                     EPQPPPILVSYLEPVYSTASSIVSTNKLDYQPLVSQINS"
     gene            complement(1885..2775)
                     /gene="djlA"
                     /locus_tag="VAH76_000656"
     CDS             complement(1885..2775)
                     /gene="djlA"
                     /locus_tag="VAH76_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEO1385304.1"
                     /translation="MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRG
                     LVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVAKSDGRVSEQEISMAKSIMNEMK
                     LSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
                     SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQGYKHTSSSQGQQ
                     GYKPQSPPNTLAHAFALLEVSPNANKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLA
                     NDKTHQIMKAYELICETKGW"
     gene            2930..4189
                     /gene="waaA"
                     /locus_tag="VAH76_000657"
     CDS             2930..4189
                     /gene="waaA"
                     /locus_tag="VAH76_000657"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEO1385305.1"
                     /translation="MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQE
                     NAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVTTMTPTGSERVKSRFGHKVAHQY
                     LPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
                     LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQY
                     RELKNHWGEDRLTVIAASTHDDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQ
                     LSVQAGFNTGCRSNLDTVSRENEVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVL
                     EPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIQLYQDKESQNTMV
                     ANASSVLESNKGSVVRYLQKIESVLGY"
     gene            complement(4194..5027)
                     /locus_tag="VAH76_000658"
     CDS             complement(4194..5027)
                     /locus_tag="VAH76_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1385306.1"
                     /translation="MFFMYFTNYGYRLNQIKKGSDQHWLFLPGGPGLGSEYLASFCDK
                     LTLQGTVSLVDFPKDGNNGEGQLDFISWQEGLISLLKSLNKPILVTHSFSGMFTLNFP
                     EIENHLSGLVLMNTTTTNSFFQHVNEMRQKHNLPDLVPAASQYHLYPSNETYKKFWDT
                     YKHYCFTQEELPIGEQMMELFAYNNESYHYAIEHFYNDFHYKWHPQTIPTMTIASELD
                     YICPPAIFIQDKNFQCQNVINKVIYKAGHCPWILHLEQVQKCFDEFFGMIQEINMERI
                     L"
     gene            complement(5097..5573)
                     /gene="dksA"
                     /locus_tag="VAH76_000659"
     CDS             complement(5097..5573)
                     /gene="dksA"
                     /locus_tag="VAH76_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEO1385307.1"
                     /translation="MTGQLIDKTKERLYNVKNDAIGNMGIAPYQETEGEEYMNEKQLA
                     HIEKILLAWRQSLMEEVDRTVSHMKDEAANFPDPSDRASQEEEFSIELRTRDRERKLI
                     KKIEDALERLRNEDFGYCEACGIEIGLKRLEARPTATLCIDCKTLSEIKERQNQGA"
     gene            complement(5672..6535)
                     /gene="prmC"
                     /locus_tag="VAH76_000660"
     CDS             complement(5672..6535)
                     /gene="prmC"
                     /locus_tag="VAH76_000660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO1385308.1"
                     /translation="MIDIRSLLEQALQQFKVKNQETKLEAELLLCHVLNKNRAYLFAH
                     PDALVSPEQIETYLQMIKQRAEGLPIAYITGQREFWSLSLKVTPNVLIPRHETERLVE
                     LALELIPDKENVSVLELGTGSGAIALALAKERPLWHIDACDFSKEALELARYNAKTLG
                     LNNINFCHSYWFNNLPLKQYHAIVSNPPYIAENDPHLKKGDVRFEPTSALVSSQDGLA
                     DLQYIIQHSYEYLLPDGLLLVEHGFEQKNEISAILNQLGYKNIHCWQDLQGHDRVSGG
                     QRPSNRNNKPL"
     gene            complement(6528..7616)
                     /gene="prfA"
                     /locus_tag="VAH76_000661"
     CDS             complement(6528..7616)
                     /gene="prfA"
                     /locus_tag="VAH76_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO1385309.1"
                     /translation="MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQ
                     LEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMAEEEIDIVKKQIEELDEQLQWHL
                     IPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
                     YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQ
                     INPDDLRIDTYRSSGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLL
                     KTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDI
                     MEGNLSLVIDPLKREYHAELLADLGRHD"
     gene            complement(7600..8874)
                     /locus_tag="VAH76_000662"
     CDS             complement(7600..8874)
                     /locus_tag="VAH76_000662"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEO1385310.1"
                     /translation="MVFVACGLNHKTAPIHVREKVALQPAMQDSLLSSLLDLPEVNEA
                     AILSTCNRTEIYCDTNTPEVLGNWLAHEHQLSEELLSQFLYIHQGKEGIKHTLRVASG
                     LDSMMIGEPQILGQMKQAYQHACRLGTVKTQLRPVFEYIFRASKRIRTRSGIGANPVS
                     IAYAAVQLIGQLFKNYHSLSVFLIGSGETASLVAKYLHQHGVHRFLIASRTLENAQKL
                     AETFGGKTLSIGDIPQYLPLADVVISATACPLPFINKSLVEHALEQRNHAPMFLLDLA
                     VPRDIEGNVNELEQVHLYNVDDLQSMIEKGMNERRNAALQAEQLIESELDNYIRWHRS
                     LRAKDVICDYRNQMHTLAQQELQRALKKISAGQNQQDVLNEFSMRLVNKLTHNPTIGL
                     RQIAWDNREDLLDLARYLFDTTANQSLYEEIS"
     gene            complement(8998..9177)
                     /locus_tag="VAH76_000663"
     CDS             complement(8998..9177)
                     /locus_tag="VAH76_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385311.1"
                     /translation="MTHVVQYVAIHVGGKTQQDFRKSLFSLLDMSPFEDRESKLNPLP
                     ANFPKRDNLEEEEDG"
     gene            9195..9473
                     /locus_tag="VAH76_000664"
     CDS             9195..9473
                     /locus_tag="VAH76_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385312.1"
                     /translation="MDIILQGQHNAIETVESLERVLKLFKERYQVDGFREIHLTVTLV
                     DEQGDEVELVDSDTNQPYRIFEVYRKGYELAGRKGIPMLQLVVDNTHK"
     gene            9530..10603
                     /locus_tag="VAH76_000665"
     CDS             9530..10603
                     /locus_tag="VAH76_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEO1385313.1"
                     /translation="MIMALVAIILTLILVIGIHELGHALAARLFQVKISKISIGFGKP
                     LIQWQTQSGCNWIWAMWPLGGYVQLLNSRISPVKPQENAYCFDKKPIWIRVLILLSGA
                     IANLITAWIALVLVFYIGISYKQPQIQSVKLDSLAAKAGIQAGDQWVSVENYPTDSWQ
                     GVGMQLVIHWGQKNVQIVLRQANQQLKQLSLDLSQIEFTSKDGSLLESLGIKPDLSAV
                     SSLTRYPSLLASMQKAFAEIIHTMYFFIMILKQLFLGVIPFSILLGPLAIFSVSVASL
                     TQGVIVFLLFIATLSLAVALVNLFPIPGLDGGSILYSVIEKIRGKPVSVAVEVLLHRL
                     MIILFCVLLVHLLMNDLNRYLHH"
     gene            complement(10632..11222)
                     /locus_tag="VAH76_000666"
     CDS             complement(10632..11222)
                     /locus_tag="VAH76_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO1385314.1"
                     /translation="MKQLGSCCQYCAYPLSDDTYLVCGQCVRKRPSFDRAYIAYRFEE
                     PLRNLIHQFKYHNGLYLASFLKQLLLDALPKSALKPDCLIPVPMHPKRLKRRGFNQAA
                     VLTRLLARQLKIPYDLYYCQKIINTASQANLDGEQRRKNLRHAFYVSPVTYEHVMIVD
                     DLLTTGSTANEMAHTLKNAGVKRVDICCCARAVTKN"
     gene            11380..12234
                     /gene="bioC"
                     /locus_tag="VAH76_000667"
     CDS             11380..12234
                     /gene="bioC"
                     /locus_tag="VAH76_000667"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEO1385315.1"
                     /translation="MTVNNEISKAFSKHADDYERVAKVQKEIGSRLFERLQYLKIAPR
                     RILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQARKKQGWRRKWPLISADMQKM
                     PFATGAFDLVFANQVIHWSSSLGTVFRELNRVMNVNGCLMFTTLGPDTFKELQTAWSA
                     ANQYAHVNEFVDMHDIGDCLVAEHFMDPVIDMELLSIHYETLPQLLLALKTQGVRNIN
                     PKRNHGLTGKSAWKQFEAQYATMRTTTGKYPLTYEVVYGQAWKGEQRKMEQGIETWIP
                     VSRVVRKS"
     gene            complement(12278..13174)
                     /locus_tag="VAH76_000668"
     CDS             complement(12278..13174)
                     /locus_tag="VAH76_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO1385316.1"
                     /translation="MNKQVIDKYTQRHELYLEQLLNEIIIPAPQIRSALHYALFSGGK
                     RIRPILVYLAGDLIDVDQRVLDIIAAALELTHCYSLIHDDLPAMDNDDLRRGKPSCHK
                     AFDEATAILVGDGMQALAIEVVLTRLSPLLPAAQVVAITQVLVNASGISGMVSGQSLD
                     LSELAKSSVTEEQLREIHLLKTGKLILACFEMVLAAQHEVSEQIKSALRTYGKHIGLV
                     FQMQDDYLDLYAPTQILGKGRSSDQANQKTTFATLFNKQQLEEEIAVHYQIAMDSLRL
                     FGSKAAALIELTKQLQNRSNLS"
     gene            complement(13155..13385)
                     /locus_tag="VAH76_000669"
     CDS             complement(13155..13385)
                     /locus_tag="VAH76_000669"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO1385317.1"
                     /translation="MSKGIHFEQSITELEEIVRQLEKGELSLEESLKQFEKGISLARR
                     CQNALNQAEQKIETLTGTDSNIELDSDEQTSD"
     gene            complement(13579..13962)
                     /locus_tag="VAH76_000670"
     CDS             complement(13579..13962)
                     /locus_tag="VAH76_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385318.1"
                     /translation="MPAFKKGQNPVTELTRLKEYMEDQIAKAKESSSLTAQLKFLENA
                     HTEHFVKMGSLTTIYKGGSEVVDCLKIEIRSLYEEMLELKDKCRDQIQQQDASMKHSP
                     AFFTTRNNKTKTEEFDLDFDKAFGL"
     gene            complement(14225..15103)
                     /locus_tag="VAH76_000671"
     CDS             complement(14225..15103)
                     /locus_tag="VAH76_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1385319.1"
                     /translation="MAKFTVGQDPVKGLTELKAYMEEQISKIKKATSEQDIDQLLIDT
                     LNEYDDKMGSLSKVYKGGNEQVEQLKIDVRKLYEPLRDQFSHKHPLTLVSEVQTKLEE
                     QRKQAEVEKLKREEQLKLEKQVEEEKLKLAKQSEEEKPKLENQQEETITQALQKVDGI
                     IQELTLKIDRVDQHQYKKAHDTANTLLQSLIAARDEYERDLRANEFSQELAGRKFKLA
                     CQDAVKIAKPVLEKDLGWGDYLKNLLKCLGNAVITVFTFGYQQGFFAYARPDSAKAVE
                     KAEEDLGLSQAASSPK"
     gene            15339..16691
                     /gene="radA"
                     /locus_tag="VAH76_000672"
     CDS             15339..16691
                     /gene="radA"
                     /locus_tag="VAH76_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO1385320.1"
                     /translation="MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGLVPENKTL
                     RSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKS
                     TLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
                     KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEG
                     TLAGPRVLEHMVDSVLYFEGQNDSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANP
                     SAIFLSRQPEPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAIL
                     LAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDSETIIFGE
                     VGLAGEIRPVQSGQERIKEAIKHGFKRAIVPFANAPKQQLDSMTVEPVKHLRDVLEKM
                     "
     gene            16688..17524
                     /locus_tag="VAH76_000673"
     CDS             16688..17524
                     /locus_tag="VAH76_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEO1385321.1"
                     /translation="MSYQKPITPEYKMNEDTIRDFNRDGAVCLRQFLNAEEIAILKEG
                     IEFNLSSLSPRAKIASRPDDPGYFVEDFCTWQNNPFYQRIIFGSNLGVAAAQIMQSQS
                     VRLYHDHLLVKEPGTRQVTPWHQDQPYYNIEGKQNCSFWIPVDPVSRSSTLEFIAGSH
                     LGPWLMPRTFLDNQAKWFPEGSLQELPPIEEARARYPIIGWEIEPGDVVCFHMLTLHM
                     ARGVEGRHRRRVFSVRFLGDDITHAPRAWPTSPDFPGLTAEIPAGASMDHPLFPCLPL
                     KI"
     gene            17812..19740
                     /locus_tag="VAH76_000674"
     CDS             17812..19740
                     /locus_tag="VAH76_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-gulono-gamma-lactone oxidase"
                     /protein_id="HEO1385322.1"
                     /translation="MALPTELKSKFIQILNQNKISHSVGWSNYMGGVNNPAAIVVEVK
                     NEKEVQTVMKAIKELNSDREPKDKVTVRATAGWADKPGKSCCFFPWSEAQESQYNEGF
                     SFSQVVGGKESPDTAGTDVIIRFAKKFHHAKVIGSLKNPPVFNPDNPIHRLPVTLVEV
                     SAGMQVADFADFLREHQLSLSTVSMISWVTPVGLAGTAGHGTGRDEPAFSGLIESVTV
                     CDMDGNLREINSDHPDFKDLTGGHSGLLGIVVSMKLRAVKAFNLCETIELFPNTREMT
                     GRLSDILKNNQYISIMGMPSYGCPESDKLVSKWQIRQWNYTTDKPGKKEKAPYDPDVR
                     SFAQELQVRIGASVMDFLLDSGLKHLLPSFMLLSAAVVTGTRGTSPKVDYENHITHPQ
                     VAFPKEMRDVSYLIPVKDDEAGQKLELILQKMEAMLNAAGSKGEFPVTYAIYVRYLKG
                     TNGGLSTSFTSSDDERILAIDVVTHPEAPGITNFEKGFMSFLKEMKIIPRNHLGKNFP
                     AGVVHYDQFLGVERVSKYKQALERFYCSPGKNDGAERLSMSPFYTPYLQGMLSASAAT
                     NQLVGENKYEQRIERLHEEHSNLECAEFLSKLYAEVSRMSTSSEEALIAKKGFLEACK
                     AELEHRNSRLEAPVPLLS"
     gene            19830..20951
                     /locus_tag="VAH76_000675"
     CDS             19830..20951
                     /locus_tag="VAH76_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEO1385323.1"
                     /translation="MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGVTSPIGQVPIKS
                     NQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGE
                     IPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
                     PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYA
                     ETGHLCISTLHANNANQALDRVINFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQR
                     VAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQALFNLYKEGKITKENA
                     IKYADSKNNVGLQIRLGEEKNFNIQDENLQIQPDDLDKI"
     gene            21038..22963
                     /gene="ankK"
                     /locus_tag="VAH76_000676"
     CDS             21038..22963
                     /gene="ankK"
                     /locus_tag="VAH76_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkK/LegA5"
                     /protein_id="HEO1385324.1"
                     /translation="MPRVYNLKDIYLGSPSFSGHEVYLDAVYYPSDPSEKNFRVIYKK
                     NKFGNANLSRMEVAFSQLARLFLDNGLTSFQKMVVNDANKVQGLIVEHLNYVIENKEG
                     LKQPFYTLNAPKNECDCTEKRVTNSNEIPFYFLDKLPQGFFNQLLAAEKNNKLSIDYA
                     SLASILATSYTLEEDDLHKGNFGFYLVKKQGKPRVVFFKIDHDLMFVDSIMSFTTRRF
                     CHLFDGCDAFDITEEDLLKFPNLKYSANGYWPTKTSFFYKPWDNKDYRTYAEIQAFAD
                     LSHVEEFNKAKWRSFYKHILISQSQMEATLKACFDENNSSDRAHISLVIQAMLARQAR
                     LKAMLFSLKDFRDFILSQNGKERDLLCHEILNNLPEEERKSFENEIRQSLDYNHNLCC
                     SGLFEDGDTPLHIAIKLGDYRYDETIGMYGQFINMKNSSGKTPLDIALQMAGQSKVHP
                     ADVRKDYRFIMKHLLANGANQTKQFEEFDKIENIRSYQFHTPYLNKAIKAKTYHELKE
                     VLRDIGEDHQYCLKFKKMLAVECVSEFIKANQDNLSLRGILLKLKKEVDGKGTKSENA
                     ALMYIRQLRSRLWIVRQIRGLYGWSTTQGEIDYMIDKELVRLDTKNLKRLSLFDSRDS
                     STLDNVFLDISLSKNKI"
     gene            complement(23056..24351)
                     /locus_tag="VAH76_000677"
     CDS             complement(23056..24351)
                     /locus_tag="VAH76_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEO1385325.1"
                     /translation="MSSNESAAIVSPIVNQKLSQWKKQDTIWMLSLYGTAIGAGTLFL
                     PIDAGLNGILPLIIMTILAFPMTYYSHRALCRFVLSGSSSHNDITDVVEEHFGSFAGK
                     MLTILYFFAIYPILLMYSVAITNTTESFLINQMGITPPPRALLAIILILGLMGIVRLG
                     QEIIVKSMSVLVYPFATILFLLSLYLIPNWNNAIFIQSNSLHANGGHGLLMTLWLIIP
                     VMVFSFNHSPIISSFAVNQKQQYGSQADEKSSLIMKYSHLMMVFSVMFFVFSCVLSLS
                     PQDLLQAKQQNISILSYLANHFNTPIMAYIAPVIAFIAISKSFLGHYLGAKEGLSGII
                     VKSLKSTTGKTINKNSLNRIIEIFMILTCWVVATINPNILKMIETLGGPIIAVLLFIM
                     PMYAIAKVPAMKKYNNQISNVFVTVMGIISISAIIYGLI"
     gene            24592..25065
                     /locus_tag="VAH76_000678"
     CDS             24592..25065
                     /locus_tag="VAH76_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385326.1"
                     /translation="MFLEPGLINGVVMKRIILFMIAWGLLNSAYSSHSLPSHASILFT
                     LANTERSNSCPALLYNAKVVVDYDYNFERNMGLAQLRQLQSTKWSETLHPLGLSNYYA
                     FMSDMKPKAVQIEGGEVTIYRIIFHLYKNGDSRIYFMINQDGECIMSSDVVNVNL"
     gene            complement(25131..26069)
                     /gene="motB"
                     /locus_tag="VAH76_000679"
     CDS             complement(25131..26069)
                     /gene="motB"
                     /locus_tag="VAH76_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEO1385327.1"
                     /translation="MSEINDISDPINDDLEKKDKREKKEKNTHYTPIIRKIKKNDHKH
                     HGGSWKIAYADFVTAMMAFFLLMWLVASLNKAQKAGIAEYFKQPMKMALFGGESMGNR
                     KATIKGGGPNIEDTDGQVSATNKPLINESIAKDKEIVESQANEIKKLEELKSEINLSM
                     EKDPSLAGLKKQLLMDVVSDGLRIQLIDNQKKPMFDVGSDKINPEIEPILANIAKLLN
                     SVPNKITIQGHTDANPYHNPEELEYTNWELSAQRANAARRALIKAGMDENKVMTVTGY
                     SSTVLLDKKNPYNPENRRISIIVMKKGAENKIKVNK"
     gene            complement(26079..26984)
                     /gene="motA"
                     /locus_tag="VAH76_000680"
     CDS             complement(26079..26984)
                     /gene="motA"
                     /locus_tag="VAH76_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEO1385328.1"
                     /translation="MLIIVGYIVILLCVFGGFSLTGGHLAAIFQPVELLIIGGAAIGS
                     FIVGNNITVLKSVLKSLPATFRGQKSAKQASSDLLSLLYHLLSKARQQGLMSLENDID
                     EPDKSPIFTSYPNLMSNHHLIEFICDYLRLIITSNMQPFQLESLMDMEIESHHEEEMI
                     PANAITKLADAMPAFGIVAAVLGVVHTMESISLPPSELGVLIAHALVGTFLGILIGYG
                     FVGPIATAMEQNANQNQLMLQCIKVTILASLHNNPPIIATEFGRKVLFTSQRPSFNQL
                     NDEIKNAKTAQTTTQNSEATEATSS"
     gene            complement(27014..28174)
                     /locus_tag="VAH76_000681"
     CDS             complement(27014..28174)
                     /locus_tag="VAH76_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1385329.1"
                     /translation="MTRTKKSARQIEYRHIACSFQGGGALGAYQAGVLHALNEVGYHP
                     HWYVGTSIGAINAAIAAGNSDKERIEKMYQFWESIATPSAVNESALLDDKISRKIQHL
                     IAAHSALLLGQPGFFVPRFPSPDFGWHNSPDLLSYYDTSPLHNSLEKFIDFDRLNNNK
                     LTRISVGAVEVSSGMMTYFDSYKQKIGPEHIMASGALPPGFPAVKVDGKYYWDGGISS
                     NSPVNYILNHNNQKQMLCFMIHLFDSFGMVPTSMDEVLKRKKDIEFSSRFIKLINLHK
                     EIHDLRNTIHILSNHISPEEKSKPEIRECIKKGRPSAISLVRFLYEHDETELSSKDYE
                     FSKKSIHERIQKGYDDGKNAIKKSPWNQSVPMTEGIALYDMSAKDTMKKYQD"
     gene            complement(28180..28962)
                     /locus_tag="VAH76_000682"
     CDS             complement(28180..28962)
                     /locus_tag="VAH76_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEO1385330.1"
                     /translation="MKLKNKVAIVTGAASGIGKEIALVYAKEGAKVAIADLNLDQASH
                     VAEEIKSSGGQAMAVAMNVTDEAQVNQGVDKVADHFGGVDVLVSNAGIQIIESVDKLA
                     FSDWKRMLAIHLDGAFLTTKACLKYMYASGNGGSIIYMGSVHSKEASVLKAPYVTAKH
                     GLLGLCKVVAKEGGTHKVRANVICPGFVRTPLVDKQIPEQAKELGISEEDVIKKVMLK
                     ETVDGEFTTTDDVAQTALFFASFESNALTGQSLVVSHGWFME"
     gene            complement(29238..29492)
                     /locus_tag="VAH76_000683"
     CDS             complement(29238..29492)
                     /locus_tag="VAH76_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385331.1"
                     /translation="MKKIIPTLFFIGISTTMMVGCTNTQVGTAVGAAAGAGIGYGVSG
                     GSALGTAIGAGAGALVGGAIGQEQDRREYYYYSGYPYYYY"
     gene            29704..30576
                     /locus_tag="VAH76_000684"
     CDS             29704..30576
                     /locus_tag="VAH76_000684"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEO1385332.1"
                     /translation="MFSGSIVALVTPMRNDSVDVHHLRELVEFHIAKGTHALVAAGTT
                     GEAGTLSHSEKLLVIKTVIEQAKERVPVIAGTAMNATKDCIELTQQAMEYGAHAALIM
                     TPAYIKPTQEGLYLHYSHIAQSVAIPIILYNVPGRTACDMLPETVARLSKISNIIGIK
                     EATGQMTRLQQILRLCEGSIDVYSGDDLTAAQWLLAGAKGVISVTANVAAKLMAKMCD
                     LAMDDDQAGCLRIQEQLMPLHELLFVESNPIPVKWAMKKMGLIGGELRLPMTELSEKH
                     HQALEKVLKNLELI"
     gene            30591..30821
                     /locus_tag="VAH76_000685"
     CDS             30591..30821
                     /locus_tag="VAH76_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385333.1"
                     /translation="MKKLSFIVLAALALTACNSRYASNGENLYLQSRNGEALVVPPPL
                     TSANISNFYDLPQQTQDARVSIAPPVEDITTS"
     gene            30831..32123
                     /locus_tag="VAH76_000686"
     CDS             30831..32123
                     /locus_tag="VAH76_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEO1385334.1"
                     /translation="MSPSESGELSIIQELSQRIVEAQRQIRILDSIKWDESVKQEFFK
                     FRGKKLPSIDKAYYDGKPLPFKVKDKQEEFRIILRDAQNQLGQYSTVTRLIRRQCEEY
                     SRAAQMLSLRGTPSFSEMAMELYGSPDDVFYVGGPRMCEMGSLLFDVLKNLQVQLQSQ
                     ADVKRYTPIQAQEILQERLEKFFDKHPGRVSVMVSDDMIADASAGADSIKLSQNAMFS
                     DRDLRYLEVHEGWVHVGTTLNGALQPNCFFLSKGSPSSSVIQEGLAVITEVVTFSSYP
                     GRMLKITNRVIALDKVTQGADFIDIYRYFLECGLSEEDSYNQSVRIFRGSTPTGGPFT
                     KDLSYAKGFVLIYNYIRFAIGQKRVDTIPLLFTGKLVLDDLPLLGELKEREILTDPVY
                     LPPPFQDLAALSAWMSFSLYLNQFDLNEIQKNFRFLLV"
     gene            complement(32127..32429)
                     /locus_tag="VAH76_000687"
     CDS             complement(32127..32429)
                     /locus_tag="VAH76_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385335.1"
                     /translation="MRIKIILCGLILTNLTYAQGCIVGGQASQPRYVCFDGRTLDLTE
                     TGLVWNAKRGCFISSVPCCNYNATHYAYHPNPAKIGGAYARCRHDYPFTLGQMQTH"
BASE COUNT         9442 a         5854 c         6812 g        10334 t
ORIGIN      
        1 caatctatta tttaaggggt ttcatgaaac tattagcatc tttgtgtgct tttttattgt
       61 cgggagttgt tagcgcgaca ccacttaata acatagttgt atttggtgat agtttgtcgg
      121 ataacggtaa cttatatgaa tacatgaaac accaacttcc tcaatccccc ccttattttg
      181 aaggtcgttt ttccaatggc ccagtatgga tagaacgtct ggcagcttca tacttcccaa
      241 atgatcccaa ttctcatttg ctggattatg cttttggtgg ggcaggagtc tctgtcgatg
      301 aagaagatga tgaagtattt tttaccttaa gaagagaagt gaattcttat ttgttagcgc
      361 atcaggataa agcaagccct gacagtcttt ttgttatttg gataggggca aataattacc
      421 tcggaatgcc tgttgaagtg gaagaaacgc ttaaaaatgt taaccgaggg attgcggata
      481 gcatacaacg tttggtagac aagggcgcga agcatatttt agttctcaat ttacctgatt
      541 tgggaagaac tcctgccgcc ctggagtttg gctctgttga agagatgacc tatttctcag
      601 cacagcataa taatgcatta tccaatacag tcgattattt taaaaaaact tatcctgaag
      661 ttgagtggtt gttttttgac acgggtagtc attttgacca tgtgatagaa catgcttcag
      721 agtatggatt tacaaacata acaggtacct gctccttttc gatagtagat gagattacta
      781 aaaactcggt tttgaagatg gttgcgtctg tgaagccaga attgacagaa agcgcatgtg
      841 atggttattt gttttttgat ttggttcatc cgacagcgtt ggcacataaa ataatggcag
      901 aaaaagcgcg tttgatgttg gatgaagctg gtgtggagtt cgccgagaat taagagtttt
      961 ttatggcgat agcaaatgca acttgcatcg tcattcaaat gaatccgcaa tgataagagc
     1021 ggggatttta tactgagcag aacccaatga gagaatggtg tatttgaact tcacaaatat
     1081 tctctcattc taattggcac tagctagtgc gtcttgacca tcataggcaa ttttataggg
     1141 aaatatgaat caaattaaat ggatagtgct actattttgt tttgttgttt ccaatgtttt
     1201 tgcggataag cttgacataa ctgtcgatca aaaaaaaatc atattgcctt attggttatc
     1261 aagatctgtt tcttatgggg cggttatcat tgttagtggt ggagagacag cgcaatggtc
     1321 cttattattg gaacagtttg ccaagggatt gtctcgaaat ggttggtcag tcgtattatt
     1381 gaattgtact aaaaataact cgataccctg gattaaccaa ttaccggaag tcatcagtac
     1441 tttaaggcag aataataata aaagaatcgt cttagtccat tatggtgatc aacttaatct
     1501 ggcattagag tattttagca agccccaatc caagatgatc aatgggctag tgatgttatc
     1561 tgcctatgat ttaaatagaa atttgcaaaa agtacctcgg ttgcgttttc cactgtttga
     1621 tattgtgggg cagtttgatt atgacatggt aaggcagcaa cgaaagagca gggaagaaaa
     1681 atttaaagag aatagttatt tagccattga tataccaggt gctactcatg actaccaata
     1741 cagtcaacaa ttattattgg cgtttgtcca tggttggatg gcaaagcttc cagaatttga
     1801 gccacagcca ccgccgatat tggtttctta tctggaaccg gtttattcta cagcaagttc
     1861 aatcgtatcg accaacaaat tggactacca acccttggtt tcacagatca attcataggc
     1921 tttcataatt tgatgagttt tatcgttagc cagtttaatc atttcttcgg gtaaaccttg
     1981 agcaattaat ttatctggat ggttacggct taatagacgt ctgtacgctc ttctaacctc
     2041 ctgtttgttt gcattcggac ttacctcaag taatgcaaaa gcatgcgcta aagtattagg
     2101 gggtgattga ggtttgtaac cctgttgacc ttgagatgag gaggtatgct tgtacccctg
     2161 ttgattatga tagtgttgtt tggattgttc ttgctggaaa taagaaccaa aatcctcata
     2221 aaaacgatac tgtttgtgta agggagcaaa tcctagatgc gtaaaaatgt tatctagtgc
     2281 gtgaattttt tggctactta atccatctac ttgagctgcc ctatactgaa tatctacaaa
     2341 aagctttaac aaatcacggt tatccttgca gattctttgt aattggataa gagcaagact
     2401 gacattgaaa tcaggttgtt tcccttcatt aaataatcgt tttgccaaat ctttttgtcc
     2461 cttgctcaat ttcatttcat tcatgatgga tttagccatg ctaatctctt gttcagaaac
     2521 acggccatcc gatttcgcaa cgtgccccat tatggagaaa gtggcttcaa agaaagcttt
     2581 ttgaacgatt ctttgttttt ctgcatggta cagccaatgg ggattagagt aataactgac
     2641 aaggccccga tcaaaaaaat ttccaacgag aatgccaaac aaagcgccta ctggtccagc
     2701 agttaagtag ccaaagaagg caccaagtat ttttccccac caggttgtta ttacaaagaa
     2761 atcgcgtaag ttcattatca gaaattacct ataaagatat gtgttaaatt taacttcaag
     2821 ttctaataat atagattgag gacaagatag tctctaattg aattgtaaca tatcgcacaa
     2881 ggaactgttt taggtatata ctttaccttt ttgtgtttgg tgcctgttta tgcgttttgt
     2941 gtattccttt ttaatgtact tactgactcc ttacttgctt tttcgcttgt ggcggaaagg
     3001 tagaaagttg ccagcttaca ggcaaaggat aggagagcgt ttttgtctgg gaatacaaga
     3061 aaatgcacca gttgatgtat ggcttcatgc ggtttctctg ggagaagtga tcgcggcaac
     3121 accattaatt gatgcaatgc tcaacaaacg ttggtcagtg ttagtcacca caatgacccc
     3181 tacaggttct gaacgagtaa aatctcgatt tggtcataaa gtagctcatc aatatttacc
     3241 ctatgattta ccttgggttc tcaaacgatt ttttaaaaga acgagacctc gtgtcggtat
     3301 cattatggaa actgaattgt ggcctaattt aattaaccag gctcaagctt caggagtggc
     3361 attatttctg gcgaatggac gcttatctga tcgctccctg caaggatatc taaagctcaa
     3421 atttttattt aaaccggttt tgaatcaatt ttcaggtatt ttgactcaaa gcaatgaaga
     3481 tgctgagaga ttcattgcct tgggtgcaaa tgccgattta gtccatgtat tagggaatat
     3541 gaaatttgat ttacagatta actctgttga tcgtagtcaa tacagggaat taaaaaatca
     3601 ttggggagag gatagactta ccgtcattgc agcaagtact catgacgacg aggagtctca
     3661 gattttatct cagctacctc gtttgcagga agctattccg ggagtggtgt tattaattgc
     3721 tccgcgtcat ccagaacggt ttcaaacggt ctatcagttg agtgttcaag caggatttaa
     3781 tactgggtgt cgtagcaatt tggatacggt ttccagagaa aatgaagtcg ttatactcga
     3841 cagcttgggt gagcttttgg gattttatca aattagtgat tttgcttttg ttggcggcag
     3901 ccttgtcccg gttggtggtc ataatgtatt ggagccaatc gcgatgaatg tgcctgtttt
     3961 aagcgggaat caagtgcata actttaaatc aatttgcaga gagcttaaag aggctcaggc
     4021 aattttgcta gtgaatcatg ccaatgagtt ggttgatgca attatccagt tatatcagga
     4081 taaggaatct caaaacacta tggttgcaaa cgccagttca gtccttgaaa gtaataaggg
     4141 ctctgtagtg aggtatttac agaaaattga gtcagttttg ggatattagc ttgttataaa
     4201 attctctcca tattgatttc ttgtatcata ccaaaaaatt catcaaaaca tttttgaact
     4261 tgttcaaggt ggagtatcca tggacaatgg cctgctttat aaatgacttt attaatcaca
     4321 ttttgacatt gaaaattctt atcctgaata aatattgcag gtggacaaat gtaatcaagt
     4381 tcactggcaa tggtcatggt tggaatggtt tgaggatgcc acttatagtg aaaatcatta
     4441 taaaaatgct cgatggcata atgataggat tcattgttgt aggcaaataa ttccatcatt
     4501 tgctcaccga ttggtagttc ttcctgagtg aaacaataat gcttataagt atcccaaaat
     4561 tttttatagg tttcgttgga tggatataaa tgatattggc tcgcagcagg gactaaatcc
     4621 ggcaaattat gtttttgacg catttcgtta acatgctgaa agaaactgtt ggttgttgtt
     4681 gtattcatta aaacaagccc gcttaaatga ttctcaattt ccgggaaatt cagtgtgaac
     4741 attcctgaaa aactatgggt aacaaggatt ggcttgttca aagatttcag cagtgaaatg
     4801 aggccttcct gccaagatat aaaatcaagt tggccttcac cattatttcc atcttttgga
     4861 aaatcaacaa ggcttacagt gccctgcaaa gttagcttgt cgcaaaaact ggctaaatat
     4921 tcagaaccaa gccccggccc acctggcaga aacagccagt gttgatccga gccttttttg
     4981 atttgattta atctgtagcc ataattggta aaatacataa aaaacatcga ttgcttgtaa
     5041 taataaactc ccgcctggcg ggaatttatt gtttaatgtt cagaggcaag aaagaatcaa
     5101 gcaccctgat tttggcgttc cttgatttca gatagagttt tacaatctat gcataaggtc
     5161 gcagtaggtc ttgcttcaag acgtttcagg ccaatttcga ttccgcaggc ttcacaatat
     5221 ccaaaatctt catttcttaa tcgctcaaga gcgtcttcga tttttttaat gagttttcgt
     5281 tctctgtcgc gggttctcag ctcaatgcta aactcttctt cctggcttgc tctgtcggaa
     5341 ggatctggga agttggctgc ttcatctttc atgtgcgaga cagtacgatc tacttcttcc
     5401 attaaagatt ggcgccaggc gagcaatatt ttttctatat gtgccagctg tttttcattc
     5461 atatattcct cgccctcagt ctcttggtag ggcgctattc ccatatttcc tattgcatcg
     5521 tttttcacgt tgtataatct ctctttggtt ttatcaatta attgtcctgt catgccagac
     5581 cctatatttc attctgtatc tgaaactgag ttttatacaa aatcgctacg tataccaaaa
     5641 aacgtcagta tcatcaacaa aaaaataatt actacaaagg tttgttattc cgattgcttg
     5701 gacgttgtcc tccacttacc ctatcatgtc cttgaagatc ttgccaacag tgtatgtttt
     5761 tatatcctaa ttgattaagt atagcgctta tctcattttt ttgttcaaaa ccatgttcaa
     5821 ccaagagcag tccatcaggc aaaagatatt cataactgtg ctgaataata tattgtagat
     5881 cagctaatcc atcttgacta ctgactaatg cacttgttgg ttcaaagcgg acatcgcctt
     5941 tttttaaatg aggatcattt tcagcaatgt agggcggatt agatacaata gcgtggtatt
     6001 gttttagggg gagattatta aaccagtagg aatgacagaa attaatattg tttaagccga
     6061 gtgttttagc gttataacgg gctaactcca atgcctcttt gctgaaatca caggcgtcaa
     6121 tatgccaaag gggtctttct tttgcaagcg ccagggcaat agcaccactt cctgttccca
     6181 gctcaaggac agaaacattc tccttgtcag gtattagctc cagtgcaagt tccaccagac
     6241 gctcggtttc atgtcttgga atgagaacat tgggtgttac ttttaacgaa agagaccaaa
     6301 attcccgctg tccagtaatg taggctattg gtagaccttc agcacgttgt ttaatcatct
     6361 gcaaataggt ttctatctgt tcaggggaaa ccaaggcatc cggatgggca aaaagatagg
     6421 cccggttttt gtttaacaca tggcagagta aaagttctgc ctctaacttg gtttcctggt
     6481 tcttgacttt gaattgttgc agagcttgtt ccagtaatga acgaatatca atcatgtcga
     6541 cctaaatcgg ctagcagttc agcatggtat tcgcgtttta acgggtcaat gaccagggat
     6601 aaattccctt ccatgatgtc actaagctgg tagatagtca gattgatacg atgatcagtg
     6661 agccgacctt gaggaaagtt ataagttcta atgcgctcgg agcggtcacc tgtaccaacc
     6721 agtgacttac gtgtttgagc ttgttcttgt ttttgtttgc ttacttcggc atctaataac
     6781 cttgttttta acaaggacat tgctttggca cggtttttgt gctgtgaacg ctcatcctgg
     6841 cactctacaa ccactcctgt agggatgtgt gtgatacgaa tggcagaatc tgttttgtta
     6901 acatgctgtc caccggctcc agaagatctg taagtatcga tgcgtaagtc atcgggattt
     6961 atctgaatgt cattaatttc atccacctca ggcatgatag caaccgtgca tgcggaagta
     7021 tggacacgac cttgcgattc agtttcaggt actctctgta ctctgtgagc tcctgattca
     7081 aatttgagtt gtgaataaac cgcttgccca ctgattttgg caattatttc cttgtagcct
     7141 ccatgttcac catggctggc gctaattaat tcaatttgcc aaccctggct ttctgcataa
     7201 cggctgtaca ttcgaaagag atccccggca aatatagccg cttcgtctcc acctgtgccg
     7261 gctctgactt caagataaat attgcgttca tcatctggat ctttaggaat gagatgccat
     7321 tgtaattgtt catccaattc ctcaatttgt ttcttgacga tgtctatctc ttcttcagcc
     7381 atagtcgcta aatctttatc atcactttcc aacaattcgt ttaaagaatc caaattattt
     7441 tttgcctcta aatacgactc ataacattgg gaaactggtt caagttgggc atattctttt
     7501 gataaagact taaattgatt ttgatcggca ataatggatg cttcggacaa taagcggccc
     7561 acttcctcgt atcgctccaa catttgctgt aattttaact caagagattt cttcatataa
     7621 tgattgattg gctgttgtat caaagagata tcgtgcgagg tcaagtaagt cttctctgtt
     7681 atcccatgct atttgtctta aacctatggt ggggttatga gttagcttgt tgaccaagcg
     7741 catactgaat tcatttaaaa cgtcttgctg attttgacca gcggatattt ttttcaaagc
     7801 acgttgtaat tcctgttgag ccaaagtgtg catttgattg cggtaatcgc aaatgacgtc
     7861 tttagctcta agcgagcgat gccatctaat gtagttatcc agttcgcttt cgatcaattg
     7921 ttctgcttgt aatgccgcat ttcgcctttc attcatccct ttttctatca tggattgtaa
     7981 atcatctaca ttatagagat gaacttgttc caattcattg acatttcctt caatatcacg
     8041 aggaacggcc aaatccaata aaaacatagg ggcatggttt ctctgttcca aggcatgctc
     8101 aaccaggctt ttattgatga atgggagtgg gcatgctgtg gcagaaataa caacatcagc
     8161 cagaggtaaa tattgcggga tatcaccaat ggacagtgtt tttccaccga aagtttcagc
     8221 taatttttga gcattttcca gagtgcggct cgctatcaaa aaacgatgga caccatgttg
     8281 atgcaggtat ttagcaacca gagaggccgt ttctccagaa ccaattaaaa agacacttaa
     8341 ggaatgatag tttttaaata attgccctat cagttgtaca gcagcatagg caatcgatac
     8401 ggggttagct ccaattccgc tacgagttct gatgcgtttg gacgctctga aaatatattc
     8461 aaacacaggc cgtaattgtg tttttactgt gccaagacga caagcatgtt gatacgcctg
     8521 tttcatttgg ccaaggattt gtggttcgcc tatcatcatg gaatccaaac cgctggcaac
     8581 acgcaaggta tgtttgattc cttcttttcc ctgatggata tacaaaaatt gagataataa
     8641 ttcctctgat aattggtgtt catgagctaa ccagtttcca agaacctcag gggtattggt
     8701 atcacagtag atttcagtcc ggttgcatgt agataaaata gccgcttcat tgacttcagg
     8761 taaatcaagc agactgctca atagagaatc ttgcatggct ggctgcagtg cgactttttc
     8821 acgcacatga attggagcag ttttatgatt aagcccgcaa gctacaaata ccatagaatc
     8881 ttgatttatt tgtttaaggg aattaacgaa ttgtaaacga ttcattgtaa aatttgtaga
     8941 gaaaaaggaa aggttccttg tatttctgtt tcttgcaatc attttacagt atatttttta
     9001 cccgtcttct tcctcctcaa gattgtcccg cttgggaaaa tttgctggca agggattcaa
     9061 tttactttct ctatcttcaa acgggctcat atctaataat gagaacagtg actttctaaa
     9121 gtcctgttga gttttgccgc caacatgtat agccacatat tgcacaacat gtgtcatttc
     9181 aataggagct ttaaatggac attatcctgc aaggtcaaca taatgctatt gaaactgttg
     9241 agagcctgga gagggttctg aaattattta aagagcgtta ccaggttgat ggatttcgcg
     9301 agattcattt gactgtcact ttagttgatg aacagggcga tgaggtggag ttggttgata
     9361 gtgatacgaa tcaaccttat cgaatttttg aagtttatcg taaaggatat gaattggctg
     9421 gaagaaaagg gattccaatg ttgcagctgg tagttgataa tacgcataaa tagattattc
     9481 tttctggtgc ttttttaata agcctcatat tctggataag gagtccatta tgattatggc
     9541 attggtagcc ataatactta cgttaatcct ggtgataggt atacatgaac tggggcatgc
     9601 actggcagcc cgcctgtttc aagtcaaaat tagtaaaatt tccataggat ttggaaaacc
     9661 attaattcaa tggcaaaccc aaagtggttg taactggatc tgggcaatgt ggcctttggg
     9721 agggtatgtt caattactga attcgaggat tagtccagta aagccacaag aaaatgctta
     9781 ttgttttgat aagaaaccaa tttggattcg tgtgctgatt ttattgtcag gagcgattgc
     9841 caaccttatt accgcatgga tagccttggt gttggttttt tatattggca ttagctacaa
     9901 acaacctcaa attcaatcgg taaaactgga tagccttgct gccaaggcag gtattcaagc
     9961 aggcgaccaa tgggtttctg tagagaatta cccaactgat tcctggcaag gggtaggtat
    10021 gcaattggta attcattggg ggcaaaagaa tgttcaaatt gttttaaggc aagcgaatca
    10081 acaattaaaa caattgagtc ttgatttaag tcagattgaa tttacttcaa aagatggctc
    10141 tttgctcgag agtctgggga tcaagcctga cttgtcagca gtttccagtt tgacgcgcta
    10201 tccatctcta ctggcttcaa tgcaaaaagc gtttgctgag ataatccata ctatgtactt
    10261 ttttataatg atattgaagc aattgtttct cggggttatt cctttttcaa ttttacttgg
    10321 accgttggct attttttctg tttctgtggc ttctttaact caaggtgtta tcgttttttt
    10381 actattcatt gctacattga gtcttgctgt agctttggtc aatttatttc ctatacctgg
    10441 tcttgatgga ggcagcattt tatattcggt tatagagaaa attcgtggga aacctgtctc
    10501 agtggctgtg gaagtcttgc ttcatcgctt gatgattatt ctattttgtg ttttgttagt
    10561 gcatttatta atgaatgatt tgaatcgata tttacaccat tgacagtgga ctcataggta
    10621 cgggctgcct tttaattttt agttacagcc cgcgcacaac aacatatatc tactctctta
    10681 actccggcat tttttaatgt atgtgccatt tcattggctg tgctgcctgt ggttaataag
    10741 tcatcaacaa tcatgacgtg ttcataagtt acaggtgata cataaaaagc atgacgtaaa
    10801 tttttccttc gttgctctcc atcaaggttg gcttgtgagg cggtattgat tattttttgg
    10861 caataatata aatcataagg gatttttaat tgacgagcta ataatctggt taatacagca
    10921 gcctggttaa atccccttcg ttttaatcgt ttgggatgca tgggaacggg aattaggcaa
    10981 tctggtttca atgcgctttt gggtaaggca tcaagaagta attgctttag aaacgaggcc
    11041 agataaaggc cattatggta tttaaactga tgtattaaat tacgcagagg ttcttcaaaa
    11101 cgatacgcta tataagccct atcaaaggag ggcctttttc taacgcattg accacaaacc
    11161 agatacgtat catcagaaag ggggtaggca cagtactgac agcaagatcc aagttgtttc
    11221 ataaactcta tgcaattaga gcaaactgca agttggcttt tgtgaaactg gttgcaaaga
    11281 gtgcatattg aaggtaaacg taagttctgt gttatactta agaatttttg gagcacgata
    11341 gttggatttc cagcatttaa tttttccctg gtctttataa tgacggtcaa caatgaaata
    11401 agcaaggcat ttagcaaaca tgccgatgac tatgagcgtg tggccaaagt tcaaaaagaa
    11461 atcggcagtc gtttgtttga gcgattacaa tatttgaaaa ttgctcctcg acgaattctt
    11521 gatttggggt gtgggcctgg atttttttca aaagaattgg ctttgttata tccgaaagcg
    11581 cagattgttg gaatggattt gtcgtttgcc atgttagagc aagccaggaa aaaacagggt
    11641 tggcgtcgta aatggccttt aatcagtgcc gatatgcaga aaatgccttt tgcaacaggc
    11701 gcttttgatt tggtttttgc taaccaggtt attcactgga gtagctcatt aggcacagtt
    11761 tttcgggaac ttaatcgtgt aatgaatgtc aacggatgct tgatgtttac taccttgggt
    11821 cctgatacct tcaaggaatt acaaacggcc tggtctgccg ctaatcaata tgcccatgtt
    11881 aatgaatttg ttgatatgca tgatattgga gattgtttgg ttgccgagca tttcatggac
    11941 ccggtcattg acatggaatt actgtctata cattacgaga cccttcccca attgctatta
    12001 gctctaaaaa ctcaaggggt aagaaatatt aaccctaaac gaaatcacgg gctcacaggt
    12061 aaaagtgcgt ggaaacaatt tgaagcacaa tatgctacta tgcggactac taccggcaaa
    12121 tatcccttaa cttatgaagt ggtttatggg caagcctgga agggtgagca acgtaaaatg
    12181 gagcaaggta tagaaacctg gattcctgtt tcacgtgttg ttagaaagtc ctaatttaaa
    12241 acggatatca attggattgt ccggtgcatc aatggcgtca agacaaattg ctacgatttt
    12301 gtaattgttt ggttaattca attaatgcag ccgctttact accaaataag cgtagagaat
    12361 ccattgcaat ttggtaatga acagcgattt cttcttccaa ttgttgcttg ttaaataagg
    12421 ttgcaaaggt agttttttgg tttgcctgat ccgaggagcg accttttccc aatatttgag
    12481 ttggagcgta gagatccagg taatcatctt gcatttgaaa tacaagccct atatgtttcc
    12541 cataagttct tagtgcggat ttgatttgct ctgatacctc atgttgtgct gctaaaacca
    12601 tttcaaaaca cgccaggatc agttttccag ttttcaaaag atgaatttct cgtaattgct
    12661 cctcagttac tgatgatttg gccagttctg ataaatcaag actttggcca ctaaccattc
    12721 cacttattcc actggcattt accaatactt gagtaatggc tacgacttgt gctgcgggga
    12781 gaaggggcga taatctcgtc aatacgactt caatcgctaa agcttgcatg ccatcgccga
    12841 ctaaaatagc ggttgcctca tcaaatgctt tatgacaact cggcttaccg cggcgcagat
    12901 cgtcattgtc catcgccgga agatcatcat gaatcaggga atagcaatgt gttaactcaa
    12961 gcgctgctgc aataatatcc agaactctct ggtcaacatc aattaaatcg ccagctaaat
    13021 aaaccaaaat gggtcttatt cttttgccgc ctgaaaataa agcgtaatgc agtgcagagc
    13081 gaatttgggg agctggtatt attatttcat tgagcaattg ctcgaggtat agctcatgtc
    13141 tttgggtata cttatcaatc acttgtttgt tcatctgagt cgagttcaat gttggaatca
    13201 gtaccagtta acgtttcaat tttttgctct gcctgattca aagcattttg gcaacgtctt
    13261 gcaaggctaa ttcccttttc aaattgtttc agcgattctt caaggctaag ctctcccttt
    13321 tccagttgcc ggacaatttc ttcgagttca gtgattgatt gttcgaaatg tatgcctttg
    13381 ctcataactg ttttccaagg cgataaaagg agtgattata ctgggttaac gcgaggattc
    13441 aacttctgaa tggaagtaag tggaaaatca atcatagaag cttattggca gctttgggtt
    13501 ttataaagga actccagaac tggatgttgg actttgctag tttaatatca aagaccaaca
    13561 ttaggtggtt agcagttttt aaagaccaaa tgctttatca aaatccagat caaattcttc
    13621 tgtctttgtt ttgttattcc tcgtagtgaa gaacgcgggg ctgtgtttca tggatgcatc
    13681 ttgttgttga atctggtcac ggcatttatc ttttaactca agcatttctt catataaaga
    13741 acgtatttca attttcaagc agtcaactac ttcactgcca cctttgtaaa ttgtggttag
    13801 agaacccatt ttgacaaagt gttctgtgtg ggcgttttcc aggaatttta gttgagctgt
    13861 taaagaggag gactcttttg ctttcgctat ttggtcttcc atatactctt ttaatctggt
    13921 aagttctgtt accggatttt gtcctttttt gaaagcaggc atgattttga cctcacattt
    13981 taaaataatc ccttaggtaa gttaaagcaa aaacaggcaa ttgaaaagca aatattttca
    14041 tattcttttt tattagataa gtattaatgc atagtgcctg gtatagggtt aattaattta
    14101 aattgaagcc aaattgtttg tcagataagt ctatgaaaat caggcgcgat ttgtataaaa
    14161 aacctgacat tcgcatttaa acatcgtgaa gccaagcgaa tgtcaggttc ttgtcgaagt
    14221 ttaattattt gggtgatgaa gctgcttgac taagccccaa atcttcttca gctttttcta
    14281 ccgctttcgc tgaatctgga cgtgcataag cgaagaaacc ttgttgataa ccaaacgtaa
    14341 agacggtaat caccgcgttg cccaagcact ttaaaagatt tttcaggtag tcaccccagc
    14401 ctaagtcttt ttctaaaacc ggtttagcta tttttacggc atcttgacaa gctaatttaa
    14461 actttcttcc agcaagttcc tgagaaaatt cattggctct taagtctcgc tcatactcat
    14521 cacgagcagc tataagactt tggagaagtg tatttgctgt gtcatgtgct tttttatact
    14581 ggtgttgatc aactctgtct attttgagtg tgagctcttg aataattcca tcaactttct
    14641 ggagagcttg agttatagtt tcttcttgtt gattttcaag cttaggtttt tcttcttcag
    14701 attgttttgc aagttttagt ttttcttctt caacctgttt ttccagcttc aattgttctt
    14761 cacgcttaag cttttctact tcagcctgtt tgcgttgttc ttctaatttt gtttgaactt
    14821 cactaaccag tgtcaatgga tgcttatggc tgaattgatc acgcaaaggc tcataaagtt
    14881 ttcttacgtc aatttttagc tgttcaactt gctcgttgcc acctttgtaa acttttgaca
    14941 gggaacccat tttatcatcg tattcattca aagtgtctat gagcagttga tcgatgtcct
    15001 gttctgatgt tgctttttta atttttgaaa tttgctcttc catgtatgcc ttaagttcag
    15061 ttaaaccttt aactggatcc tgacctacgg tgaattttgc cataatttta tcctcggttt
    15121 ttataaaaaa atcacttcgg caaataataa atcaagatag tgtttttgaa aagtaaagtt
    15181 ttaataaata tttcgtaaat tgagtgcaat aaaaagaaaa aggggggagt tcaagataag
    15241 ataagttctg gttcatttaa aaattattta aggcaaatat gttcagcgcg agttatcata
    15301 aacgtactaa ataaaatgct tgaatcaagg caaaaattat gaaagtgaaa acccagtttg
    15361 tttgtaatca atgcgcggcc atttttaaac agtgggcagg acaatgcact caatgtggcg
    15421 cctggaattc cattgttgaa gaagggcttg tgcctgaaaa caagacttta cgaagtgggc
    15481 aatgggctaa tcagcgctct gtcatcactg ctgttgaaga cgtcgtcatg gacaaaatag
    15541 tgcgtatgga ttgtggtata tccgagctca accgggtttt gggagggggg ctggtttatg
    15601 gctcggttgt tttgattgga ggggatccgg ggatcggtaa atccacctta ctgttgcaaa
    15661 cgctagctaa tttatctatg caagaaacag tattgtatgt gacgggggaa gaatcattac
    15721 aacaagttgc catgcgtgcc caaagattgg gactaccttt ggccggattg cggctattgg
    15781 cagaaactca agttgaatcc atcattgctc aagcccaaaa ggaaaaacca cgaattattg
    15841 tcgtcgattc agtacaaacg atattcactg aagccataag ttctgctccg ggaggagtta
    15901 gtcaagtcag ggaatcagca gcacaattgg tgcgttatgc caagatgacc aatacggcca
    15961 tcttcctggt tgggcatgtc accaaggaag gaacattagc cgggcctcgg gtgttagaac
    16021 atatggttga tagtgttctc tattttgaag gacaaaatga tagccgcttc agagtcattc
    16081 gtgccattaa aaatcgtttt ggcgctgtca atgaattagg tgtatttgcg atgactgaca
    16141 aaggcctgaa ggaggtagcc aacccctcag ctattttttt atctcgccag cctgaaccta
    16201 cgcctggcag tgcagtgatg gtgacttggg aaggatcaag acctatgttg gtagaagtac
    16261 aagcattggt tgatgaggcc cacggtcagc aagcgaaacg cgttactgcg ggtcttgagc
    16321 ataatcgtct ggccattttg ttagctgttt tacaccgtca tggaggtatc gctacttacg
    16381 atcaggatgt ttttatcaat gtagtggggg gagtgaaagt gactgaaaca gcaagtgatt
    16441 tggctttgtt ggctgcagtg atttcaagct tacgtaatcg tatatttgat agtgaaacca
    16501 tcatattcgg tgaagtggga ttagctgggg aaatcagacc agtgcaaagt ggtcaagaac
    16561 gcattaagga ggctataaaa catggattca agcgtgctat agtccctttt gccaacgctc
    16621 ccaaacagca actcgattcc atgacggtag agccagttaa acatttgcga gatgtattgg
    16681 agaaaatgtg agttatcaaa aaccaatcac accggaatat aaaatgaatg aagatactat
    16741 ccgtgatttt aatcgtgatg gtgccgtatg tcttcgccaa tttttaaatg ccgaagaaat
    16801 tgctattcta aaagaaggaa tagaatttaa tttatcctcc ttaagtcccc gtgcaaaaat
    16861 agccagcaga cccgatgatc ctggttattt tgttgaggat ttttgtactt ggcaaaacaa
    16921 tcctttttat caacgaatta tctttggttc gaatttggga gtagctgctg ctcaaataat
    16981 gcaaagccaa tcagtccggt tgtatcatga tcatttgttg gtaaaggaac caggcacacg
    17041 acaggttaca ccttggcatc aggatcagcc ttattataat attgagggga agcagaactg
    17101 cagtttctgg attccggttg atcctgtcag tcgttcttca actctggaat ttatcgctgg
    17161 ttctcatttg ggtccctggt taatgccaag aacattttta gataatcagg caaaatggtt
    17221 tcctgaagga agtttgcagg agttgcctcc tatagaagag gcacgtgcaa ggtatccaat
    17281 tattggatgg gaaattgagc caggcgatgt tgtatgtttt catatgctga ctttacacat
    17341 ggcaagagga gttgagggaa ggcatagaag aagagtattt tctgtgcgtt ttttaggtga
    17401 tgatattacg catgccccca gggcatggcc tacttcacct gattttcctg gtttgacggc
    17461 cgaaataccg gcaggcgcaa gcatggatca tccattattt ccatgtttgc cactcaaaat
    17521 atagtaaaac gatatatttt atttcccttc aaaaaagagt tcattttttc aaggatgaga
    17581 tttttaagat tcctattcac aacggatgtt attccaataa gcgtacaatc aaaagggtat
    17641 attttttatc attgactacc aaaattcgtt attgtttttc attaagtatt gtattgtatt
    17701 tttgaagtgt ctttatgtat atctgatcga gaggcactgt tcatggtaca ttggtttgct
    17761 ctattaaatt tatgtttaac cgcttgagga ctaatattac aaggagtaat tatggcgtta
    17821 cctacggagt taaaaagcaa gttcattcaa attctgaatc aaaacaaaat ttctcattct
    17881 gttggttggt ctaactacat ggggggtgtt aataatccag ccgcgattgt tgtggaagtt
    17941 aaaaatgaaa aagaggttca gacagtgatg aaggcgatta aggaattgaa ttctgacagg
    18001 gaacccaaag ataaagtgac tgtgagagct actgcgggat gggctgataa accgggtaaa
    18061 agttgttgct tttttccatg gagcgaagct caggagagtc agtacaatga agggttttct
    18121 ttttcccaag tcgtaggtgg taaagagtct cctgataccg caggtaccga tgtcattatt
    18181 cggtttgcaa aaaaatttca ccatgcaaaa gtgattggtt cattaaaaaa tccacctgtc
    18241 tttaatccgg acaatcccat tcatagactc cccgtgactt tggtggaagt gagcgctggg
    18301 atgcaggtgg ctgattttgc cgattttttg cgagaacatc agttatcctt gtcaactgtt
    18361 agcatgattt cctgggtaac ccctgttggc ttggctggaa cagcgggaca tggtaccggg
    18421 cgtgatgaac cggctttcag tggcctaatt gaatcggtta cagtttgtga tatggacggc
    18481 aacctgcgtg aaattaattc tgatcatcct gatttcaaag atttgacagg tggtcatagc
    18541 gggttgttgg gtattgttgt tagtatgaaa ttacgagcgg ttaaggcatt taatttatgt
    18601 gaaacaatag aattatttcc caataccagg gaaatgacag gcaggttaag tgatatatta
    18661 aagaataatc aatacatcag tattatggga atgccaagtt acggctgtcc tgaatcggat
    18721 aaactggttt ccaaatggca aattcgtcaa tggaattata ctaccgacaa accaggaaaa
    18781 aaagaaaagg caccttatga tccggacgtt cgctcttttg ctcaagaatt acaggttcgc
    18841 attggtgcct cagtgatgga tttcttactg gactcaggat taaagcattt attaccttct
    18901 tttatgttac tgtcagcagc agtagtcaca ggtactcgtg gtacgtcacc aaaagtagat
    18961 tatgaaaatc atatcacaca ccctcaggtg gcttttccaa aggagatgag ggatgtgagt
    19021 tatttgattc cagtcaaaga tgacgaagca gggcaaaaat tggaacttat tttacaaaaa
    19081 atggaagcca tgttgaacgc agcgggtagt aagggggaat ttcctgttac ttatgcgatt
    19141 tatgttcgtt accttaaggg gactaacgga ggattatcaa ccagttttac ttcatcagat
    19201 gatgagcgta ttttagcgat agacgtagtc actcatcctg aagcgcctgg gattacaaat
    19261 tttgagaagg gattcatgtc ttttttaaaa gagatgaaga ttattcccag aaatcatttg
    19321 ggaaaaaatt tccccgcggg cgtagtgcat tatgatcagt ttttgggtgt ggagagggtg
    19381 tcaaagtata agcaggcact ggagagattc tattgcagtc caggaaagaa cgatggtgct
    19441 gagcgcctga gcatgtctcc tttttatacc ccttatttgc aaggcatgtt atctgcaagt
    19501 gcagctacca atcaattagt tggtgaaaat aaatacgagc aacgcataga gaggttgcat
    19561 gaggagcact ctaatctcga atgtgctgag tttttgagta aattatatgc cgaggtgtcc
    19621 aggatgagta cgtcttcaga agaggctctt attgcaaaaa aagggttttt agaagcatgt
    19681 aaggcggaat tggagcatag gaacagtcga ttagaagccc ctgtacctct attgagttag
    19741 gcaatcagta aaataagtac aagacagagc ctagcaaatt caggagtctc tactaaactt
    19801 aaaaatataa taatattttg agttaaccta tggatattac tccttttttt aagctcatgg
    19861 tcgataaagg tgcctcagat ttgtttttca gtgtcggggc accaccaaat attaaaattg
    19921 agggcgtcac atcacctatt ggccaggttc ctatcaaatc gaatcaaatg gcagaaattg
    19981 ttttatctat tatgaatgac gcacagcaaa aagaatttga gtctaaaatg gaactcaata
    20041 tggctttttc attagctgga gtagggcgtt tccgaatcaa tttattcagg caacgtggcg
    20101 aagtggctat ggtggtgcgt tacatcaagg gcgaaatacc ttctatagaa gatttaaatc
    20161 ttcctcaaat tttaaagtct ctggtcttgg agttgcgagg tttagtattg gttgttgggt
    20221 ccacgggatc gggtaaatca acgactctgg ccgcgatgat caaccaccgt aatgaaaatc
    20281 acagagggca tattttaaca atagaggatc ctattgaata tatacatcaa cataaaaaat
    20341 caatagttga tcaacgagag ataggtattg atactctaag ctatgacaat gcattgaaaa
    20401 acgcaatgag agaagcgcct gatgttatct taattggtga aattcgtgat cttaactcaa
    20461 tgaaacatgc gatagcttat gctgaaacag ggcatttgtg catttccact ttgcatgcca
    20521 ataacgccaa tcaggctctg gatagagtaa ttaatttttt ccccgatgag gcaagacaac
    20581 agatattatt agatttatct cttaatttaa aagcaatcat ttcattacgt ttgataccag
    20641 gagttgataa tcaacgtgtc gcagcagtgg agatcttaat taactctcct tatatttccg
    20701 atttgattga aaaagggaag atagacgata ttaaagaagt catggcaaga agcacggaac
    20761 aaggcatgca gacttttgat caggctttgt ttaatttata taaagaagga aaaatcacca
    20821 aagaaaatgc gattaagtac gctgattcaa aaaacaatgt gggtttacaa attcgactgg
    20881 gtgaagaaaa aaacttcaat attcaagatg agaatttaca aatacaaccc gatgatcttg
    20941 ataagattta attcactatg catttgtttt gtacattgtt ttgattggaa cattgtgata
    21001 aaattatagt ctaatttgtt aatttttctt ccaatccatg cctagagttt ataatcttaa
    21061 agatatttat ctgggttccc cttctttttc agggcatgaa gtttatcttg atgcggttta
    21121 ttatccttct gatccgtctg aaaaaaattt tagagttatt tataagaaaa ataagtttgg
    21181 taacgctaac ttatctcgca tggaagtggc ttttagtcag ttagcccgat tatttttaga
    21241 taatggatta acttcctttc aaaaaatggt agtgaacgat gcgaataaag tacagggatt
    21301 gatagttgag catttaaatt atgtgattga aaataaagag ggattgaagc agccgtttta
    21361 tactttgaat gctccaaaga atgaatgtga ctgtactgaa aagcgagtga ccaactcgaa
    21421 tgagataccc ttctattttc tggataagtt gcctcaaggt ttttttaatc aattactggc
    21481 ggcagaaaaa aataataaat tgagcataga ttatgcctct ttggcaagta tattggccac
    21541 ttcgtacact ttggaagaag acgatttgca taaagggaat tttggttttt atttggtgaa
    21601 gaaacaggga aaaccgcgag ttgttttttt caaaattgat catgatttaa tgtttgtaga
    21661 cagcatcatg tcatttacaa caagaagatt ctgccatttg tttgatggat gtgatgcttt
    21721 tgatatcaca gaagaagatt tgttaaaatt tcctaattta aaatattctg ctaatggtta
    21781 ttggccaacc aaaaccagct ttttttataa accatgggat aataaagatt atcgtactta
    21841 tgctgaaatt caggcttttg cggatttatc ccatgtggaa gagtttaaca aggcaaaatg
    21901 gcggtctttt tacaagcata ttttaatctc tcaatctcaa atggaggcta ccttaaaggc
    21961 ctgctttgat gagaataact cttcagacag ggcacatatt tctctggtaa ttcaagcgat
    22021 gttagccaga caggcgcgtt taaaggcgat gttgttttct ctaaaagatt ttcgtgattt
    22081 tattctttcc caaaatggaa aagagcgaga tttattatgt catgaaatcc tgaataactt
    22141 gccagaagaa gagagaaaat cttttgaaaa tgaaattaga caatcgttag attataacca
    22201 taacctctgt tgttccggtc tttttgagga tggggatacc ccactgcata tagcaatcaa
    22261 gttaggcgat tatcgttatg atgaaacgat tggcatgtat gggcaattca tcaacatgaa
    22321 aaatagctca ggaaaaactc ctttggatat cgctttacag atggcagggc agtctaaagt
    22381 tcatcctgcg gatgtgcgta aagattatcg ttttatcatg aaacatttat tggcgaatgg
    22441 agctaatcag acaaaacagt ttgaagaatt cgataaaatt gaaaacatca gatcctatca
    22501 atttcatact ccttatctaa acaaagccat aaaggctaaa acttatcatg aactgaaaga
    22561 agtactcagg gacattggtg aagatcatca gtattgtttg aaattcaaaa agatgttggc
    22621 agtcgaatgc gtttctgaat ttataaaggc caatcaagat aatctcagtt taagaggaat
    22681 actgttaaaa ttaaaaaagg aagtggatgg taagggaaca aaatcagaaa atgcggcttt
    22741 aatgtatata cgtcaattaa gaagtcggct atggattgtg cgtcaaatca gaggacttta
    22801 tggctggtct acaacgcaag gcgaaattga ttatatgatt gataaagagc tggtgcgttt
    22861 ggatactaaa aatttaaaga gactttcatt atttgattcc agagacagca gtactttgga
    22921 taatgtcttt ttggatatat cactatcaaa gaataaaatc taagttcatt ctattgagaa
    22981 aaataccaat gtttagcaat attattaagg cgtaatatac gccttaataa atatacgcaa
    23041 ctaccaggtt gattgttaaa ttaagccata aatgatggct gagatagaaa taatgcccat
    23101 gacggtaaca aatacgttac tgatttgatt gttgtatttt ttcatggctg gcactttagc
    23161 aatggcatac attggcatga taaataataa gacagctatg atggggcctc ctaatgtttc
    23221 aatcattttt aaaatattag gattaattgt agcaacaacc caacaagtca gaatcataaa
    23281 gatttcaatg attctgttca gggagttctt attaattgtt ttgcctgttg ttgattttaa
    23341 tgatttaaca ataattccac ttaaaccttc cttggcacct aaataatgtc ccagaaaaga
    23401 ttttgaaatg gctatgaatg caataaccgg tgcaatatag gccataatag gggtattaaa
    23461 atgattcgcc agataagaca aaatggaaat attttgttgc tttgcttgca aaagatcttg
    23521 aggtgacagg ctcaacacac agctaaatac aaaaaacatc acagagaaaa ccatcatcaa
    23581 gtgactgtat ttcataatta atgagctttt ctcgtcagct tggctaccat attgctgctt
    23641 ttgattcaca gcaaaagatg aaatgatagg agaatggtta aaggagaaca ccatcactgg
    23701 aataattaac cataaagtca ttaatagtcc atgccctcca ttggcatgta aggaattgct
    23761 ttggataaaa atagcgttat tccaatttgg aattaaataa agcgaaagca gaaatagaat
    23821 agtggcaaaa ggatatacca ataccgacat cgatttgaca atgatttctt gtcctaaacg
    23881 cacgataccc attaacccaa gaattaatat gattgctaaa agggctcgtg gcggaggggt
    23941 aattcccatt tgattaatca gaaaactttc cgtggtattt gttattgcga cactgtacat
    24001 aagcaaaatt ggataaatcg caaaaaagta caggatagtc agcatcttac ccgcaaatga
    24061 gccaaagtgt tcttctacaa catcggtgat gtcattgtgt gaggacgagc ctgaaaggac
    24121 aaaacgacat aaagccctat gagaataata agtcatgggg aaagccagaa ttgtcataat
    24181 gattaaaggc aaaatgccat taagaccagc atctatagga aggaaaagag ttccggcacc
    24241 aattgctgtg ccatagaggc ttaacatcca aatcgtatct tgttttttcc attgcgatag
    24301 tttttggttg actatcggag atactatagc tgcagattca tttgatgaca tgaaaaacat
    24361 cctcgtttta atactagtaa aatagcgcct cgcaagttga tcaatattaa cactacaaag
    24421 acaatatttc aacaattgcg aactaaatca tatccaactg aaatgaacaa agcaagagat
    24481 taatttaggg taaggagcga ggaaaatggc cttgccttct taataatcca tgaagctcat
    24541 tctgatctta tacttttaaa tagttgcaaa tgaattcgta ttgtaataag aatgtttctc
    24601 gaaccaggtt taattaatgg agtagttatg aaacgaatca ttttgtttat gatcgcttgg
    24661 ggtttattga attcagccta ttcttctcac tcacttccat cccatgccag cattctcttt
    24721 acccttgcca atacagagcg tagtaattca tgcccggctt tgctgtataa tgcaaaggtc
    24781 gttgttgatt atgattataa ctttgagcga aatatggggc tagctcaatt aagacagttg
    24841 caatcgacca agtggagcga aacactacat ccgctcggtt taagtaatta ttatgcattt
    24901 atgtcggata tgaagcccaa agcggttcag attgaagggg gtgaggtaac catttatcga
    24961 attatttttc atttgtataa aaatggcgat agcaggattt attttatgat taatcaagat
    25021 ggcgaatgca ttatgagttc tgatgttgtg aatgttaatt tgtaaagtaa atagtattaa
    25081 ttttaatgaa aagctctcaa aaacggagct agacttggtc aaatgccaaa ttatttattg
    25141 accttgattt tgttttctgc cccttttttc atgacaatga tgctaatgcg ccgattttca
    25201 ggattgtaag gatttttttt atcgagaaga acagtggaag aataacctgt gactgtcata
    25261 actttatttt cgtccattcc agcttttatc aatgcgcgtc ttgctgcatt cgctctttgt
    25321 gcagataatt cccaattggt gtattccaat tcttcgggat tatgataagg attggcatcg
    25381 gtgtggcctt gaatagtgat tttattgggc actgaattga gtagttttgc gatgttagcg
    25441 agtatgggtt caatttccgg attgatttta tcagagccta catcaaacat cggttttttc
    25501 tgattgtcta tcagttgaat gcgcaagcca tcggacacaa catccatcag gagttgtttt
    25561 tttagaccag caagcgatgg atctttttcc atacttaaat tgatctctga tttcagctct
    25621 tcaagttttt ttatttcatt agcttgagat tcaacgattt ctttatcttt tgctatgctt
    25681 tcgttgatta aaggtttatt ggtggcagaa acctggccat ctgtgtcttc aatattaggg
    25741 cctcctcctt taatcgttgc ttttctattc cccatgcttt ccccgccaaa caatgccatt
    25801 ttcattggct gtttaaaata ctctgcaata cccgcttttt gagctttgtt taatgaagca
    25861 actaaccaca tgagtagaaa gaaggccatc atggccgtta caaagtcagc ataggctatt
    25921 ttccaggagc cgccatgatg cttatgatca ttttttttaa tttttctgat gataggggta
    25981 tagtgagtgt ttttttcttt tttttcccgt ttgtcctttt tttccagatc gtcgttgatc
    26041 ggatcgctga tgtcatttat ttcactcatg gctgtaacct aactggacgt agcttctgtt
    26101 gcttcagagt tttgagttgt tgtctgagcg gttttggcat tttttatttc atcatttaat
    26161 tgattaaatg aaggtctttg cgatgtaaat aatactttac ggccaaattc agtagcaata
    26221 ataggagggt tattgtgcaa acttgccagg atggtaactt ttatacattg cagcatcaat
    26281 tgattttgat tggcattttg ttccatagca gtagcgatag gcccaacaaa accatagcca
    26341 attaaaatac ctaaaaaggt tcccactaac gcgtgagcga ttaaaacacc taattcagag
    26401 ggaggtaagc tgatggattc catggtatgg acaacaccta atactgctgc gacgatacca
    26461 aaagcaggca tggcatcagc cagtttggtg atggcattgg ccgggatcat ttcttcttca
    26521 tggtgagatt cgatctccat atccatcaag ctttccaatt gaaaaggctg catgttggag
    26581 gtaataatca atctcaggta atcacaaata aactctatta aatggtgatt actcattaaa
    26641 ttgggataac tggtaaaaat tgggctttta tccggctcgt caatgtcatt ttcgagcgac
    26701 attaaccctt gttgccttgc tttactgagt aagtggtata gaagacttaa caaatcactg
    26761 gaggcttgct ttgccgattt ttggccgcga aatgtagcag gcagagattt tagaacagat
    26821 tttaaaacag taatattatt accgacgata aaggaaccta tggcggcccc tccgattatc
    26881 aataattcaa cgggttgaaa tatagcggcg agatggccgc ctgtcaggct gaatccacca
    26941 aatacacaga gtagtattac aatataacct actataatta acatatccct atcctatagt
    27001 atctggtgtc caattagtct tgatattttt tcatagtgtc tttggcagac atgtcataca
    27061 gtgctatacc ttcagtcatt gggactgatt ggttccaagg cgattttttg atcgcatttt
    27121 taccatcatc ataacctttt tgaatgcgtt catgaataga ttttttggag aattcataat
    27181 cttttgaaga caattccgtt tcatcgtgct catataaaaa tcgaaccagt gaaatagcgg
    27241 aaggacgtcc ctttttaata cattccctta tttcaggttt tgatttttcc tcaggagaaa
    27301 tatggtttga taaaatgtga attgtgtttc ttaaatcatg aatttccttg tgaaggttaa
    27361 ttaattttat aaaccgactg gaaaattcaa tgtctttttt acgttttaat acctcatcca
    27421 ttgaggtggg aaccatccca aaagaatcaa acaaatgaat cataaaacaa agcatttgct
    27481 tttgattatt atgattcagg atataattga ctggtgaatt acttgatatg ccgccatccc
    27541 agtaatattt accatcaact ttaactgccg ggaagccggg aggaagtgca ccgcttgcca
    27601 taatgtgttc aggtcctatt ttttgtttat aggaatcaaa ataagtcatc attccactac
    27661 ttacttcaac ggctcctaca ctgattcggg ttaatttatt gttattcaga cgatcaaaat
    27721 caataaattt ttccaaactg ttatgcaaag gagaagtatc atagtaactt aataaatcag
    27781 ggctgttatg ccaaccaaaa tcaggggaag ggaaacgagg tacaaaaaaa cctggctgtc
    27841 ccaagagtaa agcggaatga gcagcaatta aatgttgaat ttttcgactt atcttatcat
    27901 cgaggagagc tgattcattg actgcggacg gagtagcgat agactcccaa aattgataca
    27961 ttttttctat ccgctctttg tcagagttac ctgcagcaat agccgcatta attgctccaa
    28021 ttgaggttcc cacgtaccaa tgagggtggt atcccacttc atttaaagca tgcaatactc
    28081 cagcttgata ggcacccaaa gcccctcctc cctgaaagct gcatgcaata tggcgatact
    28141 caatctgtct tgcagatttt ttagttcgag tcatggtttt tactccatga accaaccatg
    28201 actgactacc aatgattgtc ctgtaagcgc attcgattca aatgatgcaa aaaataaagc
    28261 ggtttgtgct acatcgtctg ttgtggtgaa ttccccgtca actgtttctt ttagcatcac
    28321 tttcttgatg acatcttctt cactgatgcc taattctttt gcttgctctg gaatttgttt
    28381 gtcaacgaga ggagtacgga caaagcccgg gcaaatgaca ttggccctta ccttatgagt
    28441 tccaccttcc ttggcaacta ctttgcataa acccaataga ccatgtttgg cagtaacata
    28501 aggtgctttg agtacagagg cttcttttga atggaccgag cccatataaa taatactgcc
    28561 gccattgcct gaggcataca tgtatttcaa gcaagcttta gtagttaaaa aggcaccatc
    28621 caaatgaatg gcgagcattc ttttccagtc tgaaaaggct aatttatcta ctgattcgat
    28681 gatttgaata ccggcattgc tgactaaaac atcaactcct ccaaagtgat cagctacttt
    28741 atccacgccc tgattgactt gtgcttcatc agttacattc atggcaacgg ccattgcttg
    28801 tccgcctgat gatttgattt cctctgctac atggcttgcc tgatcaagat tcagatcagc
    28861 gattgctacc tttgcccctt ctttagcata aacaagcgca atttctttac caattccgct
    28921 tgctgctcct gtgacaatag cgactttatt cttcagtttc atctttacta tccttgtcat
    28981 tgttttccat gaattgatta tagcaatgga tttttaataa tgccttatta tttgctaaca
    29041 aaaatctttg atgaattgtt taagtttttt agagagttgt ctttaaggga ggtaattgct
    29101 tcttttctgt atcatttcac caagagtttt ggttttaccc aaattagaac tatcctggcc
    29161 ctttctctat ctagaaaaga ccaggttctt atgatcgcta aaatgattaa agaatatatt
    29221 tagtcgacaa gtcaggatta gtaataataa taggggtagc cactgtagta ataatactca
    29281 cgtctgtctt gctcttgacc gatggcgccg cctaccaatg caccagctcc tgctccaatt
    29341 gcagttccca atgctgatcc tccagaaact ccatagccaa taccagcgcc cgcagctgct
    29401 ccaactgctg ttccaacttg agtatttgtg caacctacca tcatagtggt tgatatccct
    29461 atgaaaaaca acgttggtat aatctttttc atagtaaact tccttttgta agttgttgcc
    29521 ttcatatcaa gaatagtaca aaataagtta aatatgtaaa aattttacac aaatatgtgt
    29581 atggaatgga agataatgat tgctgataga gattccggtt gactaaagtg ttatataaaa
    29641 atttactcgg atgtctgttt tccagtaata tacgattatg gaccattaat ttttgagggc
    29701 aacatgttca gtggaagtat agtagccttg gtaaccccca tgcgaaatga tagtgtcgat
    29761 gttcatcatt tgcgagaact ggttgagttt cacatagcca agggaactca tgccttggtt
    29821 gcggctggca caactggtga ggctggtact ttatcacata gcgaaaaact tctggtcatc
    29881 aaaacagtca tcgaacaagc aaaagaaaga gtgcctgtga ttgcaggtac tgctatgaat
    29941 gcgaccaagg attgtattga gctaacccag caagctatgg aatatggggc gcatgctgct
    30001 ttaatcatga caccagccta tatcaagcct actcaagaag gattgtattt gcactatagt
    30061 catattgccc aatccgtagc gattccgatt attttatata acgttccggg aagaacagcc
    30121 tgcgatatgt tacccgaaac ggtagcaaga ctgtctaaaa tttcgaatat aataggaata
    30181 aaggaagcaa ccggacagat gactcgttta cagcagattt tgcggttatg cgaaggaagc
    30241 attgatgttt acagtggcga tgatttgaca gctgcccaat ggttgttggc cggggctaaa
    30301 ggtgttattt cagtcacggc taatgtggct gctaaattga tggcaaaaat gtgtgatttg
    30361 gcaatggatg atgatcaagc aggatgtttg cgtattcaag agcaattaat gcctttacat
    30421 gaacttttgt tcgtggagtc aaatccaatt ccagtgaaat gggccatgaa gaaaatggga
    30481 ttgattggtg gagaattaag attaccgatg acggagttgt ctgagaaaca tcatcaggct
    30541 ttggaaaagg tgttgaagaa tttagagttg atataactga gggttttgtt gtgaaaaaat
    30601 tgagttttat agttttggca gcattggcat taacagcatg taattctcgt tatgccagta
    30661 atggagaaaa tttatattta cagagccgta atggcgaagc tttggtagta ccacctccat
    30721 tgactagtgc caatattagt aatttttatg atttacctca acaaactcag gatgcgcggg
    30781 tgagtatagc gccgccggtg gaagatatca caacttcata aggattcatc atgtcgccat
    30841 cagaatcagg agagttgtca ataatacagg agttgtctca gcgtatcgtg gaggcgcagc
    30901 gtcaaatcag gattcttgat agcatcaaat gggatgaatc agtaaaacag gaatttttta
    30961 agttcagggg aaaaaaactt ccttctattg ataaggctta ttatgacggt aagccactgc
    31021 cttttaaagt caaagacaag caggaagagt ttcgtatcat tttgcgtgac gcacagaatc
    31081 aattagggca atactccaca gtaactcgcc taatcagacg tcaatgcgaa gagtatagcc
    31141 gcgcagccca gatgttatcc ttgaggggta ctccttcatt tagcgaaatg gctatggaat
    31201 tgtatggtag cccggacgat gtgttttatg tgggtggacc gcgtatgtgt gaaatgggtt
    31261 ccctgctatt tgatgtatta aagaatctcc aggtccaatt gcagtctcag gctgatgtca
    31321 agcgttacac tccgatacag gctcaggaaa ttttacagga gcgattagag aaattttttg
    31381 acaagcatcc tggccgagtg agtgttatgg tcagcgatga tatgattgct gatgcttctg
    31441 ctggagctga cagcatcaag ttaagtcaga acgctatgtt tagcgataga gatttacgct
    31501 atttggaagt tcatgagggc tgggttcatg tgggaacgac tctcaatgga gccttgcaac
    31561 caaattgctt tttcctgtct aaaggttctc cttccagtag cgtgattcag gagggcttgg
    31621 cagtcattac tgaagtcgtg acattttctt cttatccagg tcggatgttg aaaattacaa
    31681 accgggtgat tgccctggat aaggtgactc aaggggcaga ttttattgat atttatcgct
    31741 atttcctgga gtgcggattg tcagaagaag acagctataa ccagtccgta cgtatattta
    31801 gaggcagcac accgacaggt ggcccattta ccaaggattt atcctatgcc aagggatttg
    31861 tattaattta caattacata cgctttgcta tagggcagaa aagagtcgac acaatcccac
    31921 tattatttac cggcaagctg gttttggatg atttaccttt gctaggtgaa ttaaaagaac
    31981 gcgagatttt aacggatcca gtgtatcttc cacctccttt ccaggatttg gctgcattaa
    32041 gtgcgtggat gagtttctct ctgtatttga accagtttga tttaaatgaa attcaaaaga
    32101 attttcgatt tttgttggtt taaacattaa tgagtttgca tttggccaag agtaaaagga
    32161 tagtcgtgcc tgcaacgggc ataggctccg cctattttgg ctggatttgg gtgataggca
    32221 taatgagtgg cattgtagtt acagcaaggt actgagctga taaaacatcc ccgtttggca
    32281 ttccatacaa ggcctgtttc agtcagatcc aacgttcgcc catcaaaaca gacatacctg
    32341 ggttgagagg cctgaccgcc aacaatacag ccttgggcat aggtgaggtt tgttagaatt
    32401 aacccacaaa gaattatttt tattctcatt ctattcacct ta
//
LOCUS       DATNSN010000018        32390 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.018, whole genome shotgun sequence.
ACCESSION   DATNSN010000018 DATNSN010000000
VERSION     DATNSN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32390
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.018"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..414
                     /gene="zapD"
                     /locus_tag="VAH76_000688"
     CDS             <1..414
                     /gene="zapD"
                     /locus_tag="VAH76_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEO1385336.1"
                     /translation="EFLKTLRQIHHPNTKECEFNSPHLVLWFDSDPLMRQKTISQWVS
                     CLKDLEDTVKIYLSLLRGVTQYTPIRANNGFYQQSLSPKSLNHLILLRIDKKLKMIPK
                     IQLGHHSLTIRLYELITGQEIRDKTIEMEIAFCQI"
     gene            complement(459..1277)
                     /locus_tag="VAH76_000689"
     CDS             complement(459..1277)
                     /locus_tag="VAH76_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO1385337.1"
                     /translation="MITKQNGIYTLTLSLLLFSLQIKAATDYQTYETVCGEPTDMLSF
                     LERGGIATNPCVVPPRSVLISSGYQYQQLIGEGVQHNFPAAAIQLGLPGYFEVDLLLP
                     NYINQTVDPRIGFSQTQLIVNHVLWFNNKWVITASGTFVFPSGSASFGSPNPGGGVIG
                     ILSYNFNSQLNLTGNLGITSQSESSYDGGQSYTSVNPDLILSWTKNKISLFAEIYGQS
                     KTAPDEGSGFSTDAGVLYQVKKNIAIDFEVDQRITGLLNGVERYYGGGITIQFN"
     gene            complement(1506..1910)
                     /gene="mutT"
                     /locus_tag="VAH76_000690"
     CDS             complement(1506..1910)
                     /gene="mutT"
                     /locus_tag="VAH76_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEO1385338.1"
                     /translation="MKVAVAIIIDEKQRILITQRPFHVAHGGFWEFPGGKLEPHESPE
                     DALVREIREELGIIVNEYRFLGYVDYDYPDKHIQLIIFMVTRFTGNPLCQEGQLNMKW
                     VKKEELDINDFPKANHAIFDLIDIPELQYQVL"
     gene            complement(1907..4597)
                     /gene="secA"
                     /locus_tag="VAH76_000691"
     CDS             complement(1907..4597)
                     /gene="secA"
                     /locus_tag="VAH76_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO1385339.1"
                     /translation="MLSTLIKKMFGSRNERTLRRMEKSVMAINAFEPKMQALSNEELA
                     GKTQEFKERFNNGESLDELLAEAFATVREVSLRTLGLRHFDVQLIGGMVLHEGNIAEM
                     RTGEGKTLVATLPAYLNAISGRGVHIVTVNDYLAKRDSQWMKPIYEFLGLTVGVIYPD
                     MSHKEKQEAYKADIVYGTNNEYGFDYLRDNMAFSLTDKVQRELNFAIVDEVDSILIDE
                     ARTPLIISGAAEDSSELYIKINSLIPQLKKQEEEGDEGDYTIDEKQKQAHLTDAGHLH
                     IEELLTKAKLLDPGESLYHASNIMLMHHVNAALKAHAMFHRDIDYIVKDNQVVIVDEH
                     TGRTMPGRRWSEGLHQAVEAKEGVPIQNENQTLASITFQNFFRMYNKLSGMTGTADTE
                     AYEFQQIYNLEVVVIPTNRSMIRKDEADLVYLTQADKFQAIIEDVRECGVRKQPVLVG
                     TVSIEASEFLSQLLKKENIKHQVLNAKFHEKEAQIIAEAGRPGAVTIATNMAGRGTDI
                     VLGGSLAADLANLPADASEQEKEAVKKEWQKRHDEVIAAGGLRIIGSERHESRRIDNQ
                     LRGRAGRQGDPGSSRFYLSLEDNLMRIFASERVASMMRRLGMQPGEPIEHSLVTRAIE
                     NAQRKLEGHHFDVRKQLLDYDNVANDQRQVIYTQRASIMAMTDTQEVVEMMREEVMNS
                     LVDTYIPPQSLEDQWDPQALSDVLSDEFKIKAPVPDWIDKDHSIQPDQIKEKVLALAI
                     EHYDEKVRKVGRPVISQFEKSIILQTLDNHWREHLAAMDQLRQGIHLRGYAQKDPKQE
                     YKKEAFSLFTMMLDNLKYEVIRILSSVEIQTEEDAQVVEEQRRADQIRKMNLMHESLS
                     ENDEASETQTFRRQEKKIGRNDPCPCGSGKKYKACHGSLV"
     gene            complement(4698..5690)
                     /gene="dusA"
                     /locus_tag="VAH76_000692"
     CDS             complement(4698..5690)
                     /gene="dusA"
                     /locus_tag="VAH76_000692"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEO1385340.1"
                     /translation="MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGA
                     VQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQA
                     GRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
                     DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSH
                     LKHVDGVMLGRLACDNPYQIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLS
                     ILVKPLFNLAFGLPGAKHWKKKLMNIIQEKDTSGFSELIQYLLNLEAINQVEFV"
     gene            complement(5771..7510)
                     /gene="recJ"
                     /locus_tag="VAH76_000693"
     CDS             complement(5771..7510)
                     /gene="recJ"
                     /locus_tag="VAH76_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO1385341.1"
                     /translation="MLIKQRQQPEFTVNLPNVPDVLKRIYITRGISDESQLDKQLQTL
                     LPFHTLKGINEACLRLENALRSEQRILIIGDFDADGATSTALAITALRAMGARFVEYL
                     VPNRFEFGYGLTPAIVEVASKWQPHLIITVDNGIASFEGVDAANKMGIDVLITDHHLP
                     AESLPDACAIVNPNQPGCLFPSKSIAGVGVIFYVMLALRRHLSNCNWFNEINMPEPNM
                     ASFLDLVALGTVADVVGLDQNNRIMVNQGMMRIRQGQCREGIKALIEIAGRDCSRLRE
                     SDLGFAIAPRLNAAGRLDDMSLGIECLITTDPKMARNFCQQLDELNLERRQIESEMKE
                     QAMLALEKLSINDEKNKHLPVALCLHDKTWHQGVIGILAGRMKEKYHRPVIAFASVSE
                     HELKGSARSVPDLNIRDVLAAVDKDNPGLILKFGGHAMAAGLSISPELLNDFRSAFVS
                     EVLKHLDLSQCEGEILTDGPLQSHELNLETAQLIQQAGPWGQAFAEPVFDNVFEILDQ
                     RIVGKNHLKMTLISSQGGEQLDAIAFNVDLKSWPNHRIKYVHAAYKLDINFYQGRTRL
                     QLLIQAMNPVQQA"
     gene            complement(7500..8309)
                     /locus_tag="VAH76_000694"
     CDS             complement(7500..8309)
                     /locus_tag="VAH76_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385342.1"
                     /translation="MNVIINTESDCDSKEGKLLKSQGLAVLNLLACGGYDLVNPPVGN
                     LLKSSHNLEGDWVVLTPMHWQASHNDAVIVALDKDLRVTDEEVKYWFDLYSAYLAEEG
                     MSLYFYDKYTWLLRVDDKPPLNAKPIYQVLNKSLMPELSQLDETMYWQKFFTESQMFF
                     SSNAWKSLINGVWAWGSGKLKTKKTISICTDKHFLAVAQAYSSNVTLYDPSVNVSGFE
                     VVLLHGIDSLSELHQVEIKKIPAHWYWNNCALIKAKSHWFTRLWRSLTHAD"
     gene            complement(8314..9507)
                     /gene="alaC"
                     /locus_tag="VAH76_000695"
     CDS             complement(8314..9507)
                     /gene="alaC"
                     /locus_tag="VAH76_000695"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEO1385343.1"
                     /translation="MSQFPRIKRLPPYVFNTINQLKMEARARGEDIIDFGMGNPDQPT
                     PPHIVNKLIEVAQRPDTHRYSMSKGIPRLRRAMATWYLRHYDVHLDSETQILATIGSK
                     EGLAHLALAISGPGDTVLVPDPAYPIHTYGFIIAGANVKQIPLIDEVQFLAAVEDAIN
                     RTWPKPKALVLNFPANPSTHCVEYEFFEKVVDLAKRNNIWIIHDLAYADIVFDGYKAP
                     SILQVPGAIDIAIETYSMSKSYNMPGWRVGFACGNEELVAALTRIKSYLDYGTFTPIQ
                     VAAITALEGPDDCVLEIRNLYEKRRNLLCDGLNDIGWEVSKPKGTMFVWAPIPLNYRA
                     MGSLEFSKYLLKEAQVAVSPGIGFGQQGDGYVRFGLIENKDRIRQALRNLKALFKRDG
                     LLKAI"
     gene            9566..9931
                     /locus_tag="VAH76_000696"
     CDS             9566..9931
                     /locus_tag="VAH76_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEO1385344.1"
                     /translation="MNINLETAEQHAVQAYSDKKIQINSIIYESSLIVSKKEIITDFV
                     IKDIQDINDSYIDLLLRSKPELIIIGHLQTGKTLPVTLISQLSQKRIGIECMSIGAAC
                     RTYNVLLSEHRAVVAGFIF"
     gene            complement(9972..12176)
                     /gene="relA"
                     /locus_tag="VAH76_000697"
     CDS             complement(9972..12176)
                     /gene="relA"
                     /locus_tag="VAH76_000697"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HEO1385345.1"
                     /translation="MVRVKDTTPLMPDGSIDVEMWLHHLGSKGYLDNLELVRAACTLS
                     QLAGQDHATETGQTCLQQGLSMADLLADLEVDQETLAAAIIFENVHYADLSIDDVEEQ
                     LGHNIAKLVKGIEKMSAMNNFQALNKYPQNKNQIDNIRKMLLAMVDDVRVVLIKLAEC
                     LCILRTAGHLSETVRKQLATEAMEIYAPLANRLGIGAIKWEMEDLAFRHLHPEEYKAI
                     AKGLKAKRLERDSFVNRIVEQLEHQIRATGARHFAVYGRSKHIHSIYKKMKRKNVSLD
                     EIYDATAVRILVDTEAQCYEVLGMVHTLWKQIPAEFDDYIINPKSNGYQSLHTAVEGP
                     EGRVFEVQIRTFHMHDLAEMGVAAHWKYKEGAVSQKESHERKIEWLRDVLAWHQEMAA
                     NKGVAENVTTEFLEDRVYVFTPDGDVLDLPHGVTPLDFAYHVHSNLGHRCRGAKVNGH
                     IVPLTYQLKTGDRVEVLTGKEIKPSRDWINPHLNYLKTARAKAKVLHWFKMQDYEDNV
                     RDGRELLEKELKSLGIKSDKLNEVITALNYKKADDLYASLGRGDIKIGQIVNRLAPPD
                     TSEQNLQKFVRQQQKPEVTGSDLRIEGVGNLLTFMARCCQPVPGDEIVGYITIGRGVS
                     VHRKDCPNIIHASEKQKRRFIQVNWGTATRENYVVDVLIKAFDRSELLKDVTSLLSNE
                     KAHVYALQTQSNKHENMAYITLTVEIDGLNSLSRLLTKLEQIPNVLEARRQL"
     gene            complement(12182..13516)
                     /gene="rlmD"
                     /locus_tag="VAH76_000698"
     CDS             complement(12182..13516)
                     /gene="rlmD"
                     /locus_tag="VAH76_000698"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO1385346.1"
                     /translation="MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGE
                     VVEFQYTRIKKDFDEGKLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQS
                     HLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
                     TEINQCPILNSKIDTDIVHLRKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQ
                     DELKIREFAQQFQYKVFLQPGGLDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQ
                     VNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVER
                     AYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDP
                     ERIVYVSCNPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG"
     gene            13637..14893
                     /locus_tag="VAH76_000699"
     CDS             13637..14893
                     /locus_tag="VAH76_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEO1385347.1"
                     /translation="MLKNITRALSLSLLISHNICANEASSIETISQPTTIQFLEKFGA
                     GFANLEHKNLGDRVQLYLPGHNSKTGIVKLKLPNGSKLSYGEIVMYGGDMFGNPAKPI
                     SNCSEKNRLACFQAQFDALGTLGNKQDKRCSNPLNQITHLDRFFANLAEEIEKSKQNG
                     TNDWDYYKQHDSAISKELNKLTCGGSIISGFIPFGNYIKLAEVNYDHFVPDSLVAYKT
                     GHRFALETALKAYEKKQQGLIDEAHKLLELAYAQNAFANHYLTDSFSAGHMRTPRRAI
                     GHDILLPSVLNLLIANLMHNEDNQHGLNVVNHEGISWLAYGDGYLYKPEAELQRVIIL
                     DAMQRSADSIHSTFMNGVIPEHYPEMDVFPDYSKIEQINDTAPLFKVENGVLLKRVNN
                     KDHYDHHWTKYWSGLVTLIEFQVNKI"
     gene            14922..16307
                     /locus_tag="VAH76_000700"
     CDS             14922..16307
                     /locus_tag="VAH76_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385348.1"
                     /translation="MKKNIIMFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKL
                     VLISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARII
                     QGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRW
                     IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKT
                     DIVYSASITLLSALLLIGYTKHSRNKKNPIIKVTLLNIRTFRIAVLGNLLTRLSFGGI
                     PFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRLGYKKLLLLNTVLVC
                     FSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMST
                     VQQLSQSFGVAISALLVSLFTSQVSQHFVLTVKIFHLTFFALGMLTILSGLIFTSLKK
                     EDGKELIESPT"
     gene            complement(16429..16947)
                     /locus_tag="VAH76_000701"
     CDS             complement(16429..16947)
                     /locus_tag="VAH76_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1453 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385349.1"
                     /translation="MPRWFRRIVDEGERLLVEVTDELRDESEVHKAASQLHLRIEQVL
                     NANRARLEKPVIEAAEGFLHDLGVILEEDSMLRFPHAPVNQYLLAEKCTDIIYNYKPS
                     LESAPGIWNQIKAHINNFIEIIFGLENVLPTTQTMFAKDVSFTRCKRNLSRLKESLES
                     DAPDLLSVLVNC"
     gene            complement(17074..17925)
                     /locus_tag="VAH76_000702"
     CDS             complement(17074..17925)
                     /locus_tag="VAH76_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1385350.1"
                     /translation="MTSYEEKKVKIPGFTIALKIWNPNNPNPVLCLHGKMDNAASFDL
                     LAPLLSDRQLVAVDYPGTGLSSHYPEGVVPHWKNDAFLMCHVIKALGWKRFDIIAHSL
                     GSFLATVISIAQPKQVNKLVFLDILGPTVHIIENSMAYLPLNIESYLISGKQPRTVFS
                     SLDAAIKDRMNIGNISYQAAQALVQRGTKQDNEGWVWTFDPRLRCISATIPHEDEIRA
                     MFRAIDVPVCLILANQGVSYPESVFKGRSQSIKNLTIHRVQGGHHVHMDDPAPVAEII
                     SQYLSSK"
     gene            complement(17964..18329)
                     /locus_tag="VAH76_000703"
     CDS             complement(17964..18329)
                     /locus_tag="VAH76_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385351.1"
                     /translation="MKDLLVYVLFFIFSSLINVAWADNNLCIAINNFGTVPINVSGAN
                     SLEDHIVKPRETLILPEKEVAASCFPDWGCDVIIIPIEQNAKGLYLKQLPKGSRIIYS
                     QPDLYYVNRTANVPCIPDL"
     gene            18547..18996
                     /locus_tag="VAH76_000704"
     CDS             18547..18996
                     /locus_tag="VAH76_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEO1385352.1"
                     /translation="MSLLLTSPAFEHNDFIPDEFTCHGLDRPPPLAWENAPPNTKSFV
                     IIMDDPDALGGLWVHWVLFNIPANYNELDAAIDLPDSAVSVKNSWGKTGYGGPCPPRG
                     THRYFFRLYALDIFLSLDEKATKKDVEYAMQGHILETSELICKYTKL"
     gene            complement(19287..22238)
                     /locus_tag="VAH76_000705"
     CDS             complement(19287..22238)
                     /locus_tag="VAH76_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1385353.1"
                     /translation="MRKKNHPTSTMCIGLMTPYTGIASIYGHEIANAGMIACQQVNDA
                     GGVLGQQYQQHLTAYQVNDAGGVLGKELELVIIDDSSMPEQAVRAANSLIDEYHCQAI
                     IGNLLSNSRIDVANKVSLPRKVIHLNFSFYEGSIFNKYFFNFSALPNQQLLHLIPYLA
                     HKYGLKFYFAGTNYEWPRGSIFAAKNYLSQLNGEIVGEDYFAFGEAKAKKIIANVAKS
                     GANVFMPYFAGLDQVEIIKAFVDGGLREKIAMGLGHYDEAMMSYMPDMYRYGFYSSNT
                     YFMSVQTPENQHLLQELSKLKTITGVWPQGNGILTHFGEGAYLCVKAFAIAINQAGTL
                     NTEKIIMALSHVALKGPQGEVKMDPVTHHAHVNNYLAKSNQDGTLSIIKSYGSIPPVI
                     PLRYRESVKITQSKPQHFYEEGVAFSSMVSLKQLDDFKLILDSMTLCFIVTNSCGDIV
                     SINTTACKAFGYEEVELKNRSLFLLTPPPQREIYKNKINKFLHSKEPDPIDAGNNAIF
                     LGYTKNGSEFVLKGKFIKRRINKKIYLFVLLKDISQQIKEEENLVWQATHDPVTQLIN
                     KKLFHHRVIHALQRCQNGNKPISILYIDLEMINFISNIYGMWIEEKFLNEVGSRILSE
                     IHFGDTLARVGDERFVVLCENNENEALVLQLAEKIKDKLRMVFTFDQQKFFLSCTIGI
                     CHRLNKNDTVIDILRDAEVALYEAKRLGKNNIILFNSAIKKKVEDELMLATQLELAID
                     TKQLYFELQPIVTFNKSFLIGAEILLRWKLNNKEIPPSMFIPIAERSGAIYQIGYWVL
                     EQGCNIVAEWQKKLKKGKVPYISINISMKQLEDAAFYEKVRKIIAKTKANPYNIVLEV
                     TESALMEHEQAREVLYSLRKFGFRMAIDDFGTGYSSLKQLSAMPIDILKVDKYFIDNI
                     SKNKLSYSLVKKIVQMAHIFKTKIIIEGLERTEQYKLLAKIKPDAYQGFLFSPSVLQD
                     DFEKQYLFKNS"
     gene            complement(22690..23436)
                     /locus_tag="VAH76_000706"
     CDS             complement(22690..23436)
                     /locus_tag="VAH76_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal large subunit pseudouridine synthase
                     B"
                     /protein_id="HEO1385354.1"
                     /translation="MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAG
                     PSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRW
                     VQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
                     LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLT
                     MPRFLSRGQFYELTPKEVTEFLDSLPNELS"
     gene            complement(23426..24007)
                     /gene="scpB"
                     /locus_tag="VAH76_000707"
     CDS             complement(23426..24007)
                     /gene="scpB"
                     /locus_tag="VAH76_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEO1385355.1"
                     /translation="MIDNELKNIVEALILSSNEPVTLEKIQETFDEWQRPTLEDLQAI
                     IESLKVDYSSRAFELIQVASGFKVQTKSKYATWVARLQIEKPAKYSRALLETLAIIAY
                     KQPVTRADIEDIRGVAVNSQIIKTLMEREWIRIAGYKDVVGKPAVYTTTKEFLNYFNL
                     NYLSELPPLPEIMDTLTLHEATQPIKECTQNEQ"
     gene            complement(24009..24800)
                     /locus_tag="VAH76_000708"
     CDS             complement(24009..24800)
                     /locus_tag="VAH76_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO1385356.1"
                     /translation="MNIDLQPAPVVKAVVDGKEITQFPEDLYIPPDALEVLLDSFTGP
                     LDLLLYLIRKQNIDILDIPMTSITNQYLQYIQLMENRRMELAAEYLLMAAMLAEIKSR
                     LLLPVTPDSEEEEEDPRMTLVRQLQAYEQIKQAAELLDGLPRQDRDNFTIQVSPDEVE
                     AIKVYPEVELSELVEAMKVLLKREEHYSKHQVSRELMSVRERMSRILFQLQHQKTLEF
                     SQLYTLEEGRMGLVVSFLAILELAKQSLLIITQTQPFSPIHLQAA"
     gene            complement(24800..26014)
                     /locus_tag="VAH76_000709"
     CDS             complement(24800..26014)
                     /locus_tag="VAH76_000709"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO1385357.1"
                     /translation="MSALFTSNKRVVSGMRVSGRLHLGHYHGVLKNWIKLQHQYDCFF
                     FAADWHGLTTQYESPNFIEKHLWDMIIDWLACGVNPGLCKIFIQSWVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKEKDLSTYGFLGYPLLQSADVLLYHADYVPVGEDQVA
                     HIELTREIARRFNYLYGREANFEELAAAAIKKMGKKNGKIYTELRRQFQEHGIHESIK
                     TAQALLEDQPNLSIGDKERLLGYLEGSGKIILSEPQPLLTETAKMPGLDGQKMSKSYH
                     NTIMLRDEPALVEKKILTMPTDPARIKRTDPGEPEKCPVWQFHKVYSNEEVKDWVQKG
                     CRTAGIGCVDCKRPVIDAIHQELKPIQEAISDYEADLGSVKRIVAEGSEAAREEGNKT
                     LNTVREVMGLDY"
     gene            complement(26249..26869)
                     /locus_tag="VAH76_000710"
     CDS             complement(26249..26869)
                     /locus_tag="VAH76_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO1385358.1"
                     /translation="MSQFFALHPDNPQARLLRKAVSIIEEGGLIVYPTDSGYALGCSL
                     GNKSALERIRRLRQLDKHHNMTLVCRDLSQLGTYAKVSNPIFRLLKAFTPGSYTFILN
                     ATHEVPRLMLHPKRRTLGLRVPDNTITLSLLECLEAPLMSTTLILPGAEAPLSEPEAI
                     KDLLGNQIDLIIDGGNCGHEPTTVVDLTGDYPVIIREGKGDPEPFR"
     gene            complement(26866..27705)
                     /locus_tag="VAH76_000711"
     CDS             complement(26866..27705)
                     /locus_tag="VAH76_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEO1385359.1"
                     /translation="MIDLHCHSSFSDGALTPEELIKRASTLQVQCLSLTDHDTIAGYD
                     ALYDAAIHTNIEIVKGIELSTRWKKYDIHILGYQINHTAELQELIAHQNKSRIERAEK
                     IGESLHSIGIDKAYEKACLLAGHKRVGRPHFAQVLINEGKAKDMKSAFKNYLGRGKCA
                     YIPTLWISMHDAVKGISHSGGQAVIAHPLKYGLTRSKLRELITEFKEAGGVGMEVVSG
                     EMTTAQIQEMAGMCERFHLLASSGSDYHSDAQSCINLGRQRQLPIICTPIWQEWNMKQ
                     GTL"
     gene            27984..28934
                     /locus_tag="VAH76_000712"
     CDS             27984..28934
                     /locus_tag="VAH76_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO1385360.1"
                     /translation="MSSTLNSEIIKLPPLNTRELANLCGVLHSNLKILEQHFKVSIKI
                     KNHNMIICGQSGDHFEEIKKVLKLLYPLTGEPIDTKTLRVLINEDYQTAMTHHIRLSR
                     KSISARNSKQAAYLDSINTHDITFAVGPAGTGKTYLAVSKAIECFEKGEVQRLIFVRP
                     AVEAGEKLGFLPGDLVEKVLPYLRPIYDALYEMIGFKETQKLIQTDIIEILPLAFMRG
                     RTLNESFIILDEAQNTTIMQMKMFLTRMGFGSKTVITGDITQVDLPKGIDSGLAHAIQ
                     LFRHFPDISIHTFTSREVVRHPLVSKIVDCYDNEMERIKK"
     gene            28931..29407
                     /gene="ybeY"
                     /locus_tag="VAH76_000713"
     CDS             28931..29407
                     /gene="ybeY"
                     /locus_tag="VAH76_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO1385361.1"
                     /translation="MTYHIDIQNATGKLLPLSEDEITKLASLALRDHKQDAELTVRLV
                     DVEEMTYLNHTYRKKNKPTNVLAFPCSLPANIELECPLLGDVVICPEVLLAESAQFNK
                     SLHAHWSLILIHGVLHLLGYDHIKDEEASIMQMLEAKLLAELGYANPYEVEENELE"
     gene            29367..30257
                     /locus_tag="VAH76_000714"
     CDS             29367..30257
                     /locus_tag="VAH76_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO1385362.1"
                     /translation="MLIHMKSRKMNLNKLEDGGSWFSRLKQFLQGEPQNQEELVSLLR
                     DAQIRSLINSETLAMIEGAILFSKMRVRDIMLPKNQMVCINKDDDFKHIISIVTQTGH
                     SRFPVTSENSDEVVGILHAKDLLKYQPENMESFDLLDICRQVTFVPESRRLDSLLSEF
                     RSNRNHMAIVVDEYGEVSGFVTIEDIIEQIIGDIEDEFDIDEDAYIKAHEGHCYIIKA
                     HTPIEEFNEQLNADFSDETYDTIGGIVLNKFGYLPQRGEVIIIDDFEFKVLNADSRRI
                     KLLECLDKRSPEKLNSTVEG"
     gene            30265..30945
                     /locus_tag="VAH76_000715"
     CDS             30265..30945
                     /locus_tag="VAH76_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO1385363.1"
                     /translation="MSSSILIIDDDTELTDLLTQYLEPEGFNVVCVHDGENGVKKALN
                     QVFDAIILDVMLPKLNGFEVLKAIREHLETPVLMLTARGDDIDRIVGLEIGADDYLPK
                     PCNPRELVARLRAILRRTQKIPTPKPIIEQHNIIVDCSKRHVTMGGKFLELTNAEFNI
                     LEMLIKSPGQAFSKEELTEYALGRKYTAYDRSIDVHISNLRNKLGDNPQGEPLVKTVR
                     GFGYMFNA"
     gene            30938..32305
                     /locus_tag="VAH76_000716"
     CDS             30938..32305
                     /locus_tag="VAH76_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO1385364.1"
                     /translation="MRSLYWKIFVSFWLATILIIFTTAWVTSQIAQKSSQPAREEMFM
                     DSYANAAVATYESGQQSALLKWLDKIGLSRHMTLYLLTSTGEIIGAQKPPDAVKKISE
                     NLINDELPSDGIFKTGKLVVSHEILSTSGKYYRLAAVSEKPISYFVQIPWAGLTIRLT
                     LAIFISGLICYLLSRYLTQPLRSLGMAAKSIAKGNLNTRVGRFRGHSKDEIAELSNEF
                     DRMAEQLEALVHSKERLIQDISHELRSPLARLKIAIELGKKKTKHLADNEFARMEIET
                     SRLNDLIGEILDFARLEKSTTELHLSQTNIADLLAQVISDANYEFSHNSIRIQAGIIT
                     PCELNIDQRLIHRAIENIVRNASHYSPPDEKVLISSSYNKNKDQVYIDINDKGPGVPE
                     DQLEKIFNPFYRVDTSRTKKTGGYGLGLAIAARAVALHQGEIIAKNREQGGLLVRIIL
                     PANVK"
BASE COUNT         9968 a         6658 c         5589 g        10175 t
ORIGIN      
        1 gaatttttaa aaactttaag acaaatccac caccccaata ctaaagaatg tgaatttaat
       61 tcccctcatt tggtattgtg gtttgattca gatcccctaa tgaggcaaaa aacaattagt
      121 caatgggtat catgcctgaa ggatctggaa gatactgtaa aaatttattt gtcactgtta
      181 cgaggtgtta ctcagtatac tcccattaga gcaaataatg gattctatca gcaaagtctt
      241 tcacctaaat cacttaacca tctgatcttg ttaagaatag ataagaaatt gaaaatgata
      301 cccaaaatcc agttagggca tcatagttta actatcaggc tttatgagct tatcactggt
      361 caagaaatca gagataaaac aatagaaatg gaaattgcat tttgccaaat atagacgccc
      421 tatgcgctat gctggctttg cgttaaagta agtccgcatt aattaaattg tatagtaatt
      481 cctccaccat aataacgctc aacaccattc aaaaggccag tgatacgttg atcaacctca
      541 aaatcaattg caatattctt tttaacttga taaagaactc ctgcatcagt gctaaaaccg
      601 cttccttcat caggtgctgt tttactttgc ccataaatct cagcaaataa tgatatttta
      661 tttttagtcc acgataaaat aagatctgga ttaacactcg tataactttg tccaccatcg
      721 taactagatt ccgattgaga agtaataccc aaattacctg taagatttaa ttgggaatta
      781 aagttataac ttaaaattcc tattacacca cctcctggat ttggactacc aaaagaagcg
      841 ctgcctgatg gaaatacaaa tgttccgcta gcagtaataa cccacttatt attaaaccat
      901 agaacatgat tgacaataag ctgagtttga ctaaaaccta tgcgtggatc aaccgtctga
      961 ttaatataat tgggtaacaa caagtctact tcgaaataac caggcaaacc taactgtatt
     1021 gcagcagcag ggaaattatg ctgtacacct tccccaatta attgctgata ttgatatcca
     1081 gagctaatta atacgcttct aggagggact acacaaggat tagttgcaat accacctctt
     1141 tcaagaaaag aaagcatatc tgttggttca ccacaaactg tttcatatgt ttgataatct
     1201 gtagcagctt tgatttgaag tgagaatagt aataaagaaa gggtaagtgt ataaattcca
     1261 ttttgcttag taatcatttt tctgtccaat tgattaacaa ttaactgaaa tataaaatct
     1321 aatcatttac aaaaggctac tatctttaca atctctagtc tatgtgaaca taccttgtag
     1381 tgaccattct gattgatttt atctcgacaa ctgatctgtt aaaacatcat ctaaacaggg
     1441 agtttatttt gtgcttagca attaaatatg atgtcctagc ttcaaacttt agattattcg
     1501 cacatttata aaacttgata ttgaagttca gggatgtcaa ttaaatcgaa aatcgcatga
     1561 tttgctttag ggaaatcatt aatatctaat tcctcttttt ttacccactt catattgagc
     1621 tgtccttcct gacataaagg gttgcctgta aaacgagtca ccataaaaat tattaattgt
     1681 atgtgtttat ctggatagtc ataatcgaca taccccagaa aacgatattc atttacaatt
     1741 atacctaact cctccctaat ctcccgaacc aaagcatcct ctggggattc atgaggctcc
     1801 aattttccgc caggaaactc ccaaaatcct ccatgtgcga catgaaaagg acgttgggta
     1861 attagtatac gttgtttttc atcgataatg atagcaactg ctactttcac acaaggcttc
     1921 catgacaggc tttatatttt ttacctgagc cacaggggca aggatcatta cggccaattt
     1981 tcttttcttg tcgtctaaaa gtttgcgttt cacttgcttc atcattctct gataaacttt
     2041 catgcatgag attcattttt ctaatctgat ccgctctacg ttgctcttca acaacttgag
     2101 catcctcctc cgtttgaatt tcaacggatg atagtatcct gatcacctca tacttaagat
     2161 tatccagcat cattgtaaac aaactaaaag cttctttttt gtattcctgc tttggatcct
     2221 tctgagcata accccttaaa tgaattccct gacgtaattg atccattgca gccaagtgtt
     2281 cacgccaatg attatctaaa gtttgtaaaa taattgactt ttcaaattgt gaaatgactg
     2341 gccttccaac ttttcttact ttttcatcat aatgctctat agctaaagca agaacttttt
     2401 ctttaatttg atcaggttgt atactatgat ctttatcaat ccagtcaggc acaggggctt
     2461 tgattttaaa ttcatctgac aaaacatcag acaaagcctg tggatcccac tgatcctcca
     2521 gactttgagg ggggatataa gtatcaacca ggctattcat tacctcttct ctcatcattt
     2581 caacaacttc ctgtgtatcc gtcattgcca taatagaggc gcgctgagta taaatgacct
     2641 gccgctgatc attcgctaca ttatcataat ccaacaattg ctttcttacg tcaaaatgat
     2701 gcccttccag tttgcgttga gcattttcaa ttgctctggt aaccagacta tgttcaatcg
     2761 gctcacctgg ctgcattcct aaacgacgca tcatagaggc cacacgctca gaagcaaata
     2821 ttctcattaa attgtcttca agtgacaaat aaaagcggct actacctgga tcaccctggc
     2881 gaccggctcg ccctcttaat tgattatcga tacgtcttga ttcgtgacgc tcagagccaa
     2941 taattcgaag tccgcctgct gcaatcactt catcatgacg tttttgccat tcttttttaa
     3001 ccgcttcttt ttcttgctca ctcgcatcag ctggcaaatt agccagatcg gctgccaagc
     3061 taccacctaa aacaatatct gttcctcgtc cagccatgtt agtcgcaatg gttacagcgc
     3121 cagggcgccc ggcttcagca ataatttgtg cttctttttc atggaatttt gcattcaata
     3181 cctgatgctt tatgttctcc ttttttaaga gttgacttaa aaactcagaa gcttcgattg
     3241 aaactgttcc aaccaagaca ggttgtttgc gaacaccaca ttctcgtaca tcttcgatta
     3301 tcgcctggaa tttatctgcc tgtgttagat aaaccaaatc agcctcgtct tttctaatca
     3361 ttgatctatt ggtaggtatt acaaccacct caagattata tatttgttgg aactcataag
     3421 cttctgtgtc agcagtaccc gtcattcctg acaatttatt atacatgcgg aaaaaattct
     3481 gaaaagttat agaagccagc gtctgatttt cattttgaat tgggactccc tccttggctt
     3541 cgactgcctg gtgcaagcct tctgaccaac gccttccagg catggtacgg ccggtatgct
     3601 catccactat caccacttga ttgtctttga caatataatc aatatctctg tgaaacattg
     3661 catgagcctt gagtgccgca ttcacatgat gcatcaacat gatattgctg gcatgataaa
     3721 gactctcacc aggatctaat aatttggctt tggtcagcag ttcttctata tgcagatggc
     3781 ctgcatcagt cagatgagcc tgtttttgct tttcatctat agtataatcg ccttcgtcac
     3841 cctcttcttc ttgttttttt aactgtggga tcaaagaatt aattttgata tacaactctg
     3901 aactatcttc tgccgcacca gatatgatca atggtgttct cgcttcatcg atcaaaatgg
     3961 aatctacttc gtcgacaata gcaaaattaa gctctctttg taccttatca gtcagactaa
     4021 aagccatatt gtcacgtaaa tagtcaaaac cgtattcgtt attagttccg taaacaatat
     4081 ccgctttgta agcttcctgt ttttccttgt gcgacatatc aggatagata acgccaactg
     4141 taagccctaa aaattcatat atgggcttca tccattggct atctcttttc gcaagataat
     4201 cattaacagt gactatatgt acaccacgtc cgctaatggc attgagatag gctggcaagg
     4261 ttgcaaccaa tgttttacct tccccggtac gcatttcagc aatatttccc tcatgcaata
     4321 ccatcccgcc tattaattgt acgtcaaaat gcctaaggcc gagagtacgc aatgatactt
     4381 ctctaaccgt agcaaaagct tccgcaagta actcatcgag actttcacca ttattgaaac
     4441 gctctttaaa ttcttgagtt tttccagcca gctcttcgtt tgataaagcc tgcattttcg
     4501 gctcaaacgc attaatagcc attacagact tttccattcg ccgtaatgtc cgttcatttc
     4561 ggcttccaaa cattttctta attaacgtac tcagcatggc ctcaataacc ctcaggatgt
     4621 tccaattaat aaactcgtta atgtactaga aatcttgata aaattccacg aaatgttgac
     4681 atctgctcta ttatttctta tacaaactcg acttgattta ttgcttccaa atttaataaa
     4741 tactggatta attcactaaa accggaagta tctttctcct gaataatatt catcaatttc
     4801 tttttccaat gtttggctcc cggtaatcca aaagctaaat taaaaagtgg cttaaccaaa
     4861 atgcttaacg agagattggc tcgagattga tcaaaccaat aatccagata attcattaac
     4921 aactggctac gtttaatttt gatgaattct gggtataagg cttgatgaat ttcagcaatt
     4981 tgataagggt tatcgcaagc aagccgtcct aacattactc catcaacatg ctttaaatgg
     5041 ctgtttattt cttcaacagt taatatattc ccattgattg taataaagat atggggaatt
     5101 tcttttttta gttcatatac ataatcatag tttacaggag gaatcgttct attttgcttt
     5161 ggatttaacc catttaacca agccttgcga gcatgaataa taagcttatc acaacctgct
     5221 tcaaccaagt gaagagaaaa agatctgaaa aactcataac tgtcctggtt atcaataccg
     5281 atcctcgttt tcgctgtgac tggtattcct actacctgtt ttattgcacg aatacaatca
     5341 gcaacgtgtt ctggctcctt catcaagcag gctccaaatc gtcccgcctg tactttgtca
     5401 ctaggacatc ccaaattcaa attaatttca ttaaaacctt tctgttcagc aataatggcg
     5461 cacttaacta atgcggattt atctgcccca cccaattgaa tagcgatagg atgttcaaca
     5521 gaattaaaat acaaagctct gtcaggatta ttttgcacag cccctgttgt ttgcatttca
     5581 gtatacaaaa gggcgttagg agccaataac ctcatcaaag tacgaaatgg actgttagtc
     5641 caatcaatca tcggcgctat agataatttg gaaaaaatac tattaaccac gtttaatatc
     5701 aattaaaagc aaatataaca ttatacattg ttgtttgaga aatacacaaa aatagcatca
     5761 atttaagagt ttatgcttgc tgaacaggat tcattgcctg gataagcaat tggagccttg
     5821 ttctcccctg ataaaaattg atatccagtt tataggcggc atgaacgtac ttaatcctat
     5881 gattaggcca ggattttaaa tccacattaa aagcaatggc atccagttgc tcccctcctt
     5941 gtgaggaaat taatgtcatt tttaaatgat ttttaccgac aattcgttga tccagaattt
     6001 caaatacatt atcaaatacg ggttctgcaa acgcttggcc ccaaggaccg gcttgttgta
     6061 ttaattgtgc tgtttctaaa ttcagttcat gggattgcaa tggtccgtcc gtcagtattt
     6121 ctccctcgca ctgtgataag tcaagatgtt tcagaacctc tgacacgaaa gctgagcgaa
     6181 aatcattcaa taattcgggg ctaatactta aaccggcagc catagcatgt cctccgaatt
     6241 taagaattag tcctggattg tctttatcaa cagctgccag cacatctcta atgtttaaat
     6301 cggggacaga acgtgctgag ccctttaact catgctcact gacagaagca aatgcaatga
     6361 caggtcgatg atatttctcc ttcatccttc ctgccaagat tcctatcact ccttgatgcc
     6421 aggttttatc atgaaggcac aaggcaaccg gtaagtgttt attcttttca tcattaattg
     6481 acaatttctc caaagcaagc atggcttgct ctttcatttc agattctatt tgtcttcttt
     6541 ccagattcaa ctcatccaac tgttgacaga aatttcttgc catttttgga tccgtcgtaa
     6601 tcaaacattc aataccaagc gacatatcat ccagacgacc tgcggcattt aatcttggtg
     6661 ctatggcaaa accaaggtca gactctctta gcctggaaca atccctacca gcaatttcaa
     6721 tcaaggcttt tattccctct ctgcattgtc cttgacgaat acgcatcatg ccttgattaa
     6781 ccatgatcct gttattttgg tcaagcccca caacgtcggc tacagtccct aatgcaacca
     6841 aatccagaaa actcgccata ttaggctcag gcatgttgat ttcattaaac cagttacaat
     6901 tggaaagatg cctgcgcaat gccagcataa cgtaaaaaat aacgccaact cccgcaattg
     6961 acttgcttgg aaataaacaa ccaggctgat tgggattaac aattgcacaa gcatcaggta
     7021 gtgattctgc tggcaaatga tgatcagtaa ttaatacatc tattcccatt ttattagccg
     7081 catctactcc ttcaaaactg gctataccat tatcaacagt gataattaaa tgaggttgcc
     7141 atttgcttgc cacttcaaca atagcaggag taagaccata accaaattca aatcgattag
     7201 gtaccaagta ctcaacaaac cttgcaccca tagcacgcaa ggcagtaatg gctaatgccg
     7261 tagaagttgc cccatcggca tcaaaatcac cgataataag aatacgttgc tctgaacgca
     7321 aagcattctc taatcttaga caagcttcat taatgccttt taaagtgtga aaaggaagta
     7381 atgtttgtaa ttgtttatca agttgagatt cgtcactaat gcctcgtgtt atataaatac
     7441 gttttaatac atcaggtaca ttaggtaaat taacagtaaa ctcaggctgt tgtcgttgtt
     7501 taatcagcat gcgtcagact tctccataat cgagtaaacc aatgagattt ggctttaatt
     7561 aaagcacaat tgttccagta ccaatgtgcc ggtatttttt ttatttcaac ttgatgtaat
     7621 tcgcttaaag aatcaatgcc atgtaataga acaacctcaa acccgctcac gttcactgac
     7681 gggtcatata aagtcacatt tgaagaatat gcttgagcta ctgccagaaa atgcttgtct
     7741 gtgcaaattg atattgtttt tttagttttc aatttcccac taccccatgc ccaaacacca
     7801 ttaattaatg atttccaagc attggaagag aaaaacattt gactttcagt aaaaaatttt
     7861 tgccagtaca tagtctcatc cagttgcgat aattctggca tcaaagactt gtttagtacc
     7921 tgataaattg gtttggcatt aagaggtggc ttatcatcaa ctcgcaataa ccaagtgtat
     7981 ttgtcataaa aataaaggga cattccttct tccgctaaat aggcggaata caaatcgaac
     8041 caatatttca cctcttcatc tgttactcgt aaatccttat caagagcgac aatgacagca
     8101 tcattatgtg atgcttgcca atgcatggga gttaaaacca cccaatctcc ctcaagatta
     8161 tggctcgatt tcaataagtt acctactggg ggatttacaa ggtcataacc gccgcatgct
     8221 aataaattaa gtactgccaa accttgcgat tttaataatt tgccctcttt gctatcacag
     8281 tcgctctctg tgtttataat gacattcatt gcactaaata gcctttagta aaccatctct
     8341 tttaaacaga gcttttaaat ttctcaaggc ctgtcttatt ctgtctttat tttcaattaa
     8401 gccgaatcgg acataaccat ccccttgctg tccaaaacct ataccaggtg aaacagcaac
     8461 ttgtgcttct ttcaataaat atttactgaa ctcaagagac cccatagcgc ggtaattgag
     8521 aggaataggt gcccaaacaa acatggtccc tttaggtttg ctcacttccc aaccaatgtc
     8581 attcagtcca tcacacaaaa gattgcgtcg tttttcataa agatttctta tttccaacac
     8641 acaatcatca gggccttcca gtgctgtaat tgcagcaacc tggataggag taaaagtacc
     8701 gtaatccaag taggatttta tacgtgttaa ggcagctact aactcttcgt taccacaagc
     8761 aaacccaaca cgccagccag gcatattata agatttggac attgagtaag tttcaattgc
     8821 aatgtctatg gcaccaggaa cttgtaaaat cgaaggggct ttatacccat caaaaactat
     8881 atctgcgtag gccaaatcat gaataatcca aatgttatta cgcttggcaa gatcaaccac
     8941 cttttcaaaa aactcatatt cgacacagtg agtacttgga tttgccggaa aattcaaaac
     9001 caaggctttg ggttttggcc aggttctgtt aatagcatct tctactgcag ccaaaaattg
     9061 aacttcatcg attaaaggaa tttgctttac attagcacca gcaatgataa atccgtaggt
     9121 atggatagga taagcagggt caggaacaag caccgtatca cctggacctg aaattgccaa
     9181 agccaaatgt gcaagtccct ctttagatcc tattgtagct aatatttgtg tttcactgtc
     9241 cagatgaaca tcataatgtc gaagatacca ggttgccatc gctcgacgca atctcgggat
     9301 tcctttcgac attgaatatc gatgcgtatc aggcctttga gcaacctcta taagcttatt
     9361 aacaatatga ggtggggttg gttgatcagg attccccatg ccaaagtcta taatgtcctc
     9421 tcctcgcgcc cgcgcttcca tcttaagctg gtttattgta ttaaatacat aagggggtaa
     9481 gcgtttaatg cgtggaaatt gactcataat attgacctct gtgatatact gttagccatt
     9541 ataaactaat gaacttttta aaatcatgaa tatcaatcta gaaaccgcag agcaacatgc
     9601 agtgcaagct tacagcgata aaaagattca gattaattcc ataatttatg aaagtagttt
     9661 aattgtttct aagaaagaaa tcattactga ttttgtaatt aaagacatcc aggacattaa
     9721 tgattcatat attgatttat tattaagaag caaacccgaa ttaattatta ttggtcatct
     9781 gcaaacaggc aaaacgctgc ctgtcactct aatcagtcaa ttatcgcaaa aaagaattgg
     9841 tatcgaatgc atgtccatag gcgccgcctg cagaacttat aacgtcttat tgagcgaaca
     9901 cagagcagtt gttgcagggt ttatcttcta atatcaattt tttgagaagg ctctggctaa
     9961 atataccaaa gctataattg ccttcttgct tccagcacgt taggaatttg ctccaactta
    10021 gttaataagc gcgacaaact gtttaaacca tcgatctcaa cagttaaagt gatgtaagcc
    10081 atattttcat gtttattgct ctgggtctgt aaagcataaa catgggcttt ttcatttgaa
    10141 agcagcgaag taacatcctt caataactcg gagcgatcaa aggctttaat caacacatca
    10201 acaacataat tttcccgtgt tgctgtaccc caattcacct gaataaaccg tcgcttttgc
    10261 ttttcactgg catgtataat attaggacaa tcttttcgat gaacggaaac cccccttcca
    10321 atcgtgatgt atcctactat ttcatcaccg ggaacaggtt ggcagcatct cgccataaat
    10381 gtcaataagt tcccaactcc ctcaatacga agatcactcc ctgtgacctc tggtttttgt
    10441 tgttgtctta caaacttctg taaattttgt tctgaagtat ctggaggtgc caatcgatta
    10501 acaatctgac ctattttaat atcacctcgt cccaagctgg cataaagatc atccgccttt
    10561 ttataattca atgccgtaat cacttcattg agtttatccg atttaatacc taaagatttt
    10621 aattccttct ccaataactc acgaccatct ctcacattat cttcataatc ttgcatttta
    10681 aaccaatgca atactttagc cttggctctt gcggtcttta aataattcag atgaggatta
    10741 atccaatccc ttgatggttt gatttctttt ccagtcagta cttcaaccct gtctcctgtt
    10801 ttcaattgat aagtcaatgg gacaatatga ccattaacct tggcacctcg gcaacgatgt
    10861 cccaaattac tatgcacatg ataagcaaaa tccaaaggag tcaccccatg gggtaaatcc
    10921 aacacatcgc catcgggagt aaatacatac actctgtctt caagaaactc ggtcgtaacg
    10981 ttttctgcga cacctttatt agccgccatt tcctggtgcc atgccaaaac gtctcttaac
    11041 cattctattt tacgctcatg gctctctttt tgtgataccg cgccctcttt atatttccaa
    11101 tgggctgcca cacccatttc tgctaaatca tgcatatgaa aagttctgat ttgcacttca
    11161 aaaactcttc cttccggacc ctctaccgcg gtatgaagag actgatagcc attggatttt
    11221 ggattgatga tgtaatcatc aaactctgct ggaatttgct tccacaaagt atgaaccata
    11281 cccaacactt cgtaacattg tgcttcagta tctaccagaa tacgaaccgc tgttgcatca
    11341 tatatctcgt ctaatgatac atttttgcgc ttcatctttt tataaatact atggatatgt
    11401 tttgagcgac cataaacggc aaagtggcga gctcctgttg ctcgaatctg atgttcaagc
    11461 tgttcgacaa tgcgattgac aaagctgtcc ctttccaatc gtttagcctt caagcccttg
    11521 gcaatcgcct tgtattcctc tggatgcaaa tgccggaaag ccaaatcttc catttcccac
    11581 ttgattgccc ctattcccaa gcggttagct aaaggcgcat aaatttccat ggcttcggtt
    11641 gccagctgtt tgcgtactgt ttcagacaga tgccctgctg ttcttaaaat acataaacac
    11701 tccgccagct tgattaaaac aactcgcaca tcatcaacca tcgcaaggag cattttacga
    11761 atattgtcaa tttgattttt gttttgcggg tatttattta aagcctggaa attgttcatg
    11821 gcactcattt tttctatgcc tttgaccaat ttagcgatat tgtgccccaa ttgctcttca
    11881 acatcgtcaa tagataaatc cgcataatga acattttcaa aaataatagc ggcagccaga
    11941 gtttcctgat ctacttccag gtcagcaagt aaatcagcca ttgatagccc ttgttgtaaa
    12001 caagtttgcc ctgtttcagt ggcatgatcc tgaccagcca attgacttaa agtacaggca
    12061 gctcttacga gttcgagatt atccaagtat ccttttgaac caagatgatg taaccacatc
    12121 tctacgtcga tactgccatc tggcatcaac ggagtcgtat cttttactct taccatacat
    12181 attatccttt ttgaaacaag gcaatagact cgacatgcgc tgtatgagga aacatatcca
    12241 ttaccccagc tgttatcaaa acgtaacctt tttgattaac caatatatct gtatctcgag
    12301 ctaatgttat cgggttacaa gatacatata ctatacgctc gggatcaatt gaatcaattt
    12361 gttttacaat ttcaagcgca ccagaccggg gaggatcgat taaaaccttg ctaaaagacg
    12421 tattaaccag attcctaact tccatcacat catcaagatt agcggcataa aaatccacat
    12481 tggtaatatg attagacttg gcattcatat aggctctttc aaccatgttc ttattgcctt
    12541 cgacaccgat aactcgagag caatgtttag ccatgggaag cgaaaaatta cccagtccac
    12601 aaaataagtc tagcacgata tcagaatttt ttaattccat taactgaatt gcctgcgtta
    12661 ccatctttct gtttaactcg gcgtttacct gagtaaaatc gttgggatga aattgaaaag
    12721 ttatttgata atcaggcagc tcataactca aataagcatg agcatctgaa ggataaaaac
    12781 aaaaaaccga atcaagtcca cctggctgca ggaatacctt gtattggaat tgttgcgcaa
    12841 attccctgat tttcaactca tcttgctctg ttaatggaga tagattgcgg aaaatcagcg
    12901 ccacttcatt atcacctgca gccacttcaa tttgtgcgat acagtgctta tcttccatcg
    12961 tatcgattaa ttttctcaaa tggacaatat cagtatctat ttttgaatta agaataggac
    13021 attgatttat ttctgtaatg aaccttggat tattcctctc tctaaatcca accatggtgc
    13081 tttgtttctt ttctacaaag cgagtactta agcgcgcttt atttctataa ttccaatgat
    13141 ggcttgttaa aggcgacaac actgattgag gttcagtatg gccataccgg gatagtaaat
    13201 ccaaaagatg ggattgtttg aaacgaattt gttcctcggc actcatatgt tgcaaagaac
    13261 agcccccaca catttgataa tgagggcatt ttggttcaac cctgagagta gagggctcaa
    13321 caattgataa taatttacct tcatcaaaat ccttttttat ccgtgtatac tgaaattcaa
    13381 ccacctcacc aggtaatgct ccctgaataa aagttgcttt gccattaact cgtgctatcc
    13441 cccttccatc atggcttaaa tttacgatcc gagcggtttg tgaggttaaa ttcaattttg
    13501 gcttaacttt tctcatgaaa tgattctcaa ataattatca attacaaaat tctcatgcta
    13561 catttttaga atgttgtatg gatataaaaa acaaaaaatg cccgaataaa gggccaaata
    13621 atcaagggaa tgcataatgt tgaagaatat cacacgagct ttgtctttgt cattgttaat
    13681 aagccataac atctgcgcca atgaagcatc ttctatcgag acaatatctc aaccaacgac
    13741 cattcaattt ttagaaaaat ttggtgcagg ctttgccaat ctggaacata aaaatctggg
    13801 ggatagagtt caactttatt tgccaggcca taacagcaaa actggtattg taaaactgaa
    13861 attgcccaat gggagtaaac taagttatgg cgagatcgtg atgtacggtg gtgatatgtt
    13921 tggaaatcca gcaaaaccca tatccaattg ctctgagaaa aacagattag cttgtttcca
    13981 ggcgcagttt gacgctctgg ggactttagg taacaagcaa gacaaacgtt gctctaaccc
    14041 tcttaatcag ataacccatc ttgatcggtt ttttgccaat ttggctgaag agatcgaaaa
    14101 atcaaaacaa aacggaacaa acgattggga ttattataag cagcacgact cggctattag
    14161 caaggaatta aacaaactga cttgtggtgg aagtataatc agtggtttta taccctttgg
    14221 caattatatt aaattagctg aagtcaacta tgatcacttt gttcctgatt cacttgtagc
    14281 ctataaaaca ggccatagat ttgctttgga aacagcatta aaagcttatg agaaaaagca
    14341 gcagggactt atcgatgaag ctcacaagct ccttgaatta gcctatgctc aaaacgcctt
    14401 tgcaaatcat tacttaactg attctttttc tgcaggacac atgcgcactc caagacgagc
    14461 cataggacat gatatattac tgccttctgt tttaaatctt cttatcgcca atttgatgca
    14521 taatgaagat aatcaacatg ggttaaatgt agtgaatcat gaaggaataa gttggcttgc
    14581 ctatggagat ggctatttat acaaaccaga agccgaatta caaagagtga tcatcttaga
    14641 cgccatgcaa cgttctgcag acagtatcca tagtactttt atgaatggag ttatccctga
    14701 acactaccca gagatggatg tgtttcccga ttactcaaaa attgaacaga taaatgatac
    14761 agctccatta tttaaagttg aaaacggcgt tttgcttaaa cgagttaata ataaggatca
    14821 ctatgatcat cattggacaa aatactggag cgggcttgtg actttaatag agtttcaggt
    14881 taataaaatt taaacgttct aacttccata aaacaggcat tatgaagaaa aatattatta
    14941 tgttcattgt atcatgcgcc atgtttatgg aggctattga tacaactatt ctcaacacgg
    15001 caatcccggt gatggcacat agtttagaag tcaatccaat tgatctcaaa ctcgtgctca
    15061 ttagctatct tttaagtttg gcaatcttta ttcccataag tggatggatt gctgataaat
    15121 atggtgtaaa aaaagtattt atagcagcta ttggtgtctt tactctaagt tccttatggt
    15181 gcggattcac tcaaagtctt tgggaattgg tattagccag aattatacag ggtttaggcg
    15241 gctcactgac cgtgcctgtt ggacgtttaa ttatactgag aacttgtaag cgtcacgaat
    15301 taataacaaa aatgagtatt gtcgtcatgg ttgcatcgct aggtatgcta cttggcccct
    15361 tactgggcgg tatcattact gattatttct catggcggtg gatattttgg gttaatatcc
    15421 ctgttggcat aatcgccatg cttttatctc acaaactgct tccaactatc ctgcccagat
    15481 cggttccacc tttggataaa tcaggattta ttatgttcgg agcaggcctt gcattcttga
    15541 ctttaggttt gtctttgttt agtgagacga aaacggatat tgtctactcc gcttcaatta
    15601 ctcttttatc cgccttgcta ttaattggat ataccaaaca ttctcgtaac aaaaaaaatc
    15661 ctataataaa agttactttg ttaaatataa gaacatttcg tattgccgta cttggaaatt
    15721 tattaactcg tttaagtttt ggtggaattc cctttttatt acctttactc ttccaaatta
    15781 ttttagaata ttctccccag ttatcaggaa tgttacttac accagtagca ttaggcgtat
    15841 tcctggttaa accattatct ttctccatct tacgaaggtt gggttataaa aaattactcc
    15901 ttttaaatac cgttttagtc tgtttttctc tctggtcctt tgccacaata aatcaaagca
    15961 cttctgtcat ctacattggt tttttaacat ttctttatgg atttttcatt gctctgcaat
    16021 acacagggat gaattccctt gcgtatgcga atattgacga taatgatatg agttatgcaa
    16081 caagcattat gagcacagtt caacaattat cccaaagttt tggagtagca atttctgccc
    16141 tactggtcag tttattcact tctcaggttt cgcagcattt tgttttaaca gtcaagattt
    16201 ttcatctaac cttttttgct ttagggatgc ttacgattct ttcaggatta atatttacca
    16261 gcttaaaaaa agaggatgga aaagaactga ttgagtcacc cacctagatt cgcatcagtt
    16321 gcatcactaa gcagacagca ggacgaccaa gacaatgcat atcgatgcag tatctggtca
    16381 ttcaagaaaa attaatgcgt aagtaaatta attaaattca atgacgactt aacaattaac
    16441 caacacagac aacaaatcag gcgcatcact ttcaagtgat tcctttagtc tggaaaggtt
    16501 tcttttgcat ctggtgaaac ttacatcttt tgcaaacata gtctgcgtcg ttggtaaaac
    16561 attctctaaa ccgaaaatta tttcaataaa gttattaata tgagctttga tctgattcca
    16621 aatccctggt gctgactcta aggacggttt gtaattgtaa ataatatcgg tacatttctc
    16681 tgcgagtaga tattggttta ctggtgcatg aggaaatctc aacatggaat cttcttccag
    16741 gataacccct agatcatgaa gaaaaccttc agctgcttca ataaccggtt tttcaagtct
    16801 agctcgattg gcatttaaaa cttgttcgat acgtaaatgt aactgacttg ctgctttatg
    16861 cacttcgctt tcatcacgca attcatcagt tacttcgaca agcagccttt ctccctcatc
    16921 tactattctt ctaaaccatc taggcatatt aatgctccac tattaaatag attggccaat
    16981 tatacatcat gaaatcattc attatcaatc atccctgcat caacatttag agcaagagtc
    17041 catctatact tgttattctt taataaacca gttttattta gagcttaaat attgagaaat
    17101 aatttctgca acaggggctg gatcatccat atgcacatga tgtccccctt gtaccctatg
    17161 gatggtcaaa ttttttattg attgggagcg tcctttaaaa acagattcgg gataagatac
    17221 accttggtta gcgagaatta ggcaaactgg cacatctata gcacgaaaca tggctcgaat
    17281 ttcatcttca tgaggtatag ttgcactaat gcagcgtaac cggggatcaa atgtccaaac
    17341 ccagccctcg ttatcttgtt ttgtacccct ttgaaccagg gcttgagctg cttgatagct
    17401 tatattacca atattcatgc ggtccttgat tgcagcatcc agactgctaa atacagtcct
    17461 tggttgttta ccggatatta aatagctttc aatatttagg ggtaaataag ccatactgtt
    17521 ctcaataata tgaaccgttg gtcctaaaat atctaaaaat accaatttat taacttgttt
    17581 aggttgggca atacttataa cagttgccag gaaagaacct aatgagtggg caataatatc
    17641 aaagcgcttc catcctaatg ccttgataac atggcacata agaaaagcat catttttcca
    17701 atgagggaca acaccttcag gataatggct tgacaaccct gttccaggat aatctactgc
    17761 aactaattgc ctgtctgata acaaaggcgc cagcaaatca aaacttgctg cgttatccat
    17821 tttgccatgc aaacacagta ctggattagg attattggga ttccaaattt taagagcaat
    17881 agtaaaacct gggattttaa cctttttttc ttcataagat gtcaagatag atacctttta
    17941 ttaacacggg ccaatgcaaa gagtcagagg tctggtatgc atggaacatt agcagttcga
    18001 ttcacataat ataaatctgg ctgactataa ataatacgag atcccttggg aagctgttta
    18061 aggtatagtc ctttagcatt ttgctcgatt ggaataataa tgacatcaca gccccaatca
    18121 ggaaaacaag aagcagcaac ctctttttca ggtaaaatta atgtttctct aggtttgact
    18181 atatgatctt ccaaagaatt agcgccagaa acattgattg gcactgtacc aaaattattg
    18241 atagcaatgc ataagttatt atcagcccaa gctacattaa ttaatgaaga aaaaataaaa
    18301 aataaaacat agaccaataa atccttcatt aaaactctcc agttggggca aatatttaag
    18361 aggtttatat actaatgagt aggaggagta agagtcaatg aagagaacaa tattttcaat
    18421 ttcattatca ctttgatgaa tggcagatcc aaaattacca tgctaatata atttaagggt
    18481 agtagggaaa aataacaaaa agttaagatt cacaatgctg tatccaaaag agataaggag
    18541 agtattttga gtttgttact aactagcccg gcatttgagc ataacgattt tattcctgat
    18601 gaatttactt gccatggatt agatcgtcct ccgccgcttg cttgggaaaa cgctccgcca
    18661 aataccaaat catttgtcat aattatggat gatccggatg cgttaggagg attatgggtt
    18721 cattgggttt tattcaacat accggcaaac tataatgaac tggatgccgc tatagatttg
    18781 cccgattctg cggtaagcgt taagaacagt tggggcaaaa caggttacgg cggaccatgc
    18841 ccccctagag gaacacatcg atattttttt aggttatatg ctttggatat ctttttatct
    18901 ctagatgaaa aagcaacaaa gaaagatgtt gaatatgcta tgcaaggtca tattcttgag
    18961 acaagtgagc tcatatgtaa atacactaag ctctaattgc tgttgatgcc taaatcctgc
    19021 tgaatcttat aagatacact tccaaatatt ccactacctt gtttggaaga ttatctcttc
    19081 taaaatagtc aatttgtcac ttttaagaat gttaaatcta tatctttttt cccgccaaag
    19141 cttttgagat aaaggtttca cggagttcct gcatttccgc aagcctgcgt cactagagat
    19201 aaaaagattt gcatatctat agctccttga aattatggcg gctttacaag cttggtcctg
    19261 tttgaacgca gtcacaatca agacgtttag ctgttcttga ataaatattg cttttcgaaa
    19321 tcatcttgca atacagatgg agaaaataaa aagccctgat atgcatcagg tttaattttt
    19381 gctaatagct tatattgttc agtcctttca agcccctcaa tgatgatctt tgttttaaaa
    19441 atatgagcca tttgcacaat ttttttaacc aaactgtagg aaagtttgtt tttggaaata
    19501 ttgtcgataa agtatttatc tacttttaat atatcgattg gcattgctga tagttgttta
    19561 agtgatgaat agccagtacc aaaatcatcg atggccatgc gaaaaccaaa ttttcttagt
    19621 gaatataata cctcccttgc ttgttcatgc tccattaaag ctgattctgt aacttccagt
    19681 actatgttat aagggtttgc cttggttttt gcgataatct tccttacttt ttcgtaaaag
    19741 gcagcatctt ccagttgttt catagaaata ttaatggaaa tataaggtac ttttcctttt
    19801 tttaattttt tctgccattc tgcgacgata ttgcaacctt gttccaaaac ccaatagcca
    19861 atttgataga tagcaccgga acgctcagca attgggataa acatggaagg agggatctcc
    19921 ttattattaa gcttccagcg cagtaatatt tcagctccga taagaaaaga tttgttaaaa
    19981 gtcactatcg gctgcaattc aaaatataat tgttttgtat ctattgctaa ttctagctga
    20041 gttgccaaca ttaactcgtc ttccaccttt ttcttgatgg cagaattaaa gaggataatg
    20101 ttattttttc ctagtcgttt tgcttcatat aaggctactt ctgcgtccct taatatatct
    20161 ataaccgtat cgtttttatt taagcggtga caaatcccga tagtacaact taaaaagaac
    20221 ttttgttgat caaaagtaaa aaccattctt aacttatctt taatcttttc tgctaactgt
    20281 aaaaccagag cttcgttctc attattttcg cataatacaa caaaacgctc atccccaacg
    20341 cgcgccagtg tatccccaaa atggatctca cttaaaattc gacttcctac ttcattgaga
    20401 aatttttctt ctatccacat gccatagata ttagaaatga aatttatcat ttccaagtcg
    20461 atatataaaa tagaaatagg tttgtttcca ttttgacatc gttgtaaagc atggataacg
    20521 cgatgatgaa acaatttttt attgataagc tgggtaacag gatcatgtgt agcctgccaa
    20581 accagatttt cttcttcttt tatttgttga gaaatgtctt tcaacaatac aaaaagataa
    20641 atttttttat taattcttct tttgataaat ttacccttga gtacaaattc agagccattt
    20701 ttcgtgtagc ctaaaaaaat tgcattgtta ccagcatcga ttggatctgg ttcttttgaa
    20761 tgaagaaact tatttatttt atttttatag atttctcgct gaggaggagg ggtgagtaaa
    20821 aacagtgaac gattttttaa ttcgacctct tcataaccaa atgccttaca ggccgttgta
    20881 ttaatagaaa caatatcacc acaggaatta gtaacaataa aacataatgt catgctatca
    20941 agtatgagtt taaagtcatc caattgtttt aaggaaacca tggagctaaa agcgactccc
    21001 tcttcataaa aatgttgtgg tttactttgt gttatcttta cagattctcg atacctcaag
    21061 ggtataaccg ggggaattga accataagat ttgatgatgg atagcgttcc atcttgattt
    21121 gattttgcca aataattatt aacatgcgca tgatgcgtta ccggatccat ttttacctcc
    21181 ccttgtggac cttttaacgc aacgtgactt aaggccatga ttatcttttc tgtatttaag
    21241 gttccagctt gatttatggc gatagcaaat gcttttacac agaggtacgc gccttcacca
    21301 aaatgggtta aaataccatt tccttgcggc caaacgcctg tgattgtttt taatttagat
    21361 aattcctgta gcaaatgttg attttcaggt gtttgcacag acataaaata ggtgttgctg
    21421 ctgtaaaaac catatcgata catgtcaggc atataagaca tcatcgcctc atcgtaatgc
    21481 cccaacccca tggcgatctt ttctcttaat ccaccatcaa caaaggcttt aattatctct
    21541 acctgatcta agccagcaaa ataaggcata aaaacattag cgccagattt tgcgacattg
    21601 gctatgattt tttttgcttt tgcttcacca aaggcaaaat aatcttcacc aactatttcg
    21661 ccatttaatt gagaaagata attttttgca gcaaaaatag acccgcgggg ccactcataa
    21721 tttgttcctg caaaataaaa tttcaatcca tatttatgag ccaaataggg aataagatgc
    21781 agaagttgtt ggttaggtaa agctgagaaa ttaaaaaaat atttgttaaa aatgcttcct
    21841 tcataaaagg aaaaatttaa gtgaattact tttctgggaa gagagacttt gttggctaca
    21901 tcaattcgtg aatttgataa taaattacct ataatagctt ggcagtgata ctcatcgatt
    21961 agagagttcg ctgctcttac agcttgttcg ggcatgctgc tatcgtcaat gataacaagt
    22021 tccagctctt tacccaacac accaccagca tcattaacct gataagccgt caaatgctgc
    22081 tggtactgct ggcccaacac accaccagca tcattaacct gctggcaagc aatcatgcca
    22141 gcatttgcta tttcgtgacc ataaatacta gctattccgg tgtaaggcgt cattaggcca
    22201 atacacatag ttgaggtcgg atgatttttt ttacgcacgt taaaattcca tttcgtacac
    22261 agctgctttt aaacgaatgc cttattaatt tatagtgcat taacctgtgt cttgcaattg
    22321 acatactgtc tgtagtctct ttaaagattg agtatatact agcatctata gaactgggcg
    22381 acctggttcg ctactggcaa actctttaag gctaagggat tgaattgaaa tttctttgaa
    22441 atatccaata cttcaccaac taatacccat gcagtatgtc atgcaggaac tcgctctttc
    22501 tttgaagtaa aataagccac tgatttgttt ggcactgaaa ttaccaaaca aaggagtctt
    22561 ggagagaaaa attaataccg gtttgtaata tcatctctca caaaaatata attaatcaaa
    22621 aacaactgcc catgaaatta tgattctttt agtgaataca acaaaatcca acatccgttt
    22681 ttaaatcatt taagacaatt cgttgggtaa tgaatcaaga aattcagtca cttcttttgg
    22741 ggtaagttca taaaactgcc ctctggataa aaatctgggc atcgtaagca taccataacg
    22801 gattcttatt aatcgactaa cctcaacacc ttgagactcc cacaaccgac gtacttctct
    22861 gtttcttcct tcattaaggg tcacatggta ccaggaattt gttccttctc ccccacgaaa
    22921 ttgaattttg gtaaattttg ccaaaccgtc atccaacata acccctttca ataagttatt
    22981 taaagattcc tggctgactt gaccatgaac tcgtacggca tactctcttt ccaaaccata
    23041 tttgggatgc atgagttggt tagccaattc gccattatta gtaaaaatga gcaaaccgga
    23101 agtatttaaa tccaggcgcc ccacttgcac ccaacgcccc tgtctaagat gcggtaaatt
    23161 atcaaatact gttttttcaa attttggatc atgtcgactg gatacttcac cgaccggttt
    23221 atggtaaaga agaacccgga tgttttgttt gccttttaaa gggttgggaa tcaactttcc
    23281 tttgacgcta attttatcac tagggccagc ggaatctccc agtttagctg gtttgccatt
    23341 aacctgcacc caaccattat caatccaccg ctccatttca cgacgagatc caagtccggc
    23401 ttgacttaat attttctgta atctttcact gctcattttg cgtacactct tttataggtt
    23461 gggttgcctc gtgcagggtt aatgtatcca ttatttcagg taagggagga agttcactta
    23521 agtaatttaa gttaaagtaa tttaaaaatt cttttgtcgt tgtataaaca gccggcttcc
    23581 ctactacatc tttatagcct gctatacgaa tccattccct ctccattaac gtttttataa
    23641 tctggctgtt aacggcaact cctctaatgt cttcaatatc agcgcgcgta acaggttgct
    23701 tgtaagcaat tattgccaaa gtttcaagca gtgcgcgcga atactttgct ggcttctcga
    23761 tttggagacg agcaacccaa gtagcatatt tggatttcgt ttgtacctta aaaccacttg
    23821 caacctggat aagctcaaaa gctcgggaag aataatctac ctttaagctc tctattatag
    23881 cctgcaaatc ttctaatgtt ggtctttgcc actcatcaaa ggtttcttgt attttttcta
    23941 aagtaacggg ctcattactg gataatatca aagcttcaac aatatttttt aattcattgt
    24001 caatcatttt atgcagcctg caaatgtata ggtgaaaatg gctgagtttg cgtaatgatc
    24061 aataaagact gcttagccag ttcaagaata gctaaaaaac tcacaaccaa tcccatcctt
    24121 ccctcttcaa gagtatataa ttggctaaat tccaaagttt tttgatgctg caattgaaag
    24181 agaatacgac tcattcgttc acgaacagac attaactccc tggaaacttg atgtttggaa
    24241 taatgctctt cacgtttcag caaaactttc atagcctcga ctaattcaga taactcaact
    24301 tcaggataaa ctttaattgc ttcaacctca tctggactaa cctgaattgt aaaattatct
    24361 ctgtcttgtc ttggcaatcc atccaataac tcagccgctt gttttatttg ctcataagct
    24421 tgcaactgtc ttaccagagt catgcgtgga tcttcttcct cctcctctga atctggtgtg
    24481 actggcaata ataaacgtga cttgatttca gctaacatgg cagccattaa taaatactcg
    24541 gcagccagct ccattcttcg attttccatg agctggatat attgtaaata ctggttggtt
    24601 atacttgtca tgggaatatc aagaatatca atattttgct tgcggattaa atacagcaat
    24661 aaatctaatg gcccggtaaa agagtccagt aatacctcca atgcatcagg aggaatatat
    24721 aaatcctctg gaaactgagt aatttcctta ccatccacca ccgctttgac tacaggcgca
    24781 ggctgcaaat cgatattcat taataatcaa gccccatgac ttctctgacc gtatttaaag
    24841 tcttgtttcc ttcttctctg gccgcttcgc ttccttccgc tacaatacgt tttaccgaac
    24901 ctaaatcagc ttcataatct gaaattgctt cctgtattgg tttaagttct tgatggatag
    24961 catcaatcac tggtctctta cagtcaacgc aaccaattcc tgctgttcgg caaccttttt
    25021 gcacccaatc ttttacttcc tcattagaat acactttatg gaattgccaa acaggacatt
    25081 tttcaggctc tcctggatct gtacgtttaa tacgcgccgg atccgtgggc atagtcagaa
    25141 ttttcttttc aactaaagct ggctcatctc ttagcatgat cgtattatgg taagacttgg
    25201 acattttctg cccatcaaga ccaggcattt ttgcggtttc tgtaagcaaa ggctgtggtt
    25261 cacttaatat aattttgcca ctcccttcca aatatcccaa caaccgctct ttatctccaa
    25321 tcgacaaatt aggctgatct tctaaaagcg cttgtgctgt cttaatggat tcgtggatgc
    25381 catgctcctg aaattgtctg cgtaattcgg tataaatttt cccattcttt ttacccatct
    25441 ttttgattgc tgcagcagcc agctcctcaa aattagcctc tctaccataa agatagttaa
    25501 atctgcgggc tatttctcgt gttaactcaa tatgagccac ctggtcttct cccaccggaa
    25561 cataatccgc atggtataaa agtacatcag cactttgtag taaaggatat cccaaaaaac
    25621 cataggttga taaatctttt tcttttaatt tctcctgctg atctttataa gtaggaactc
    25681 gctccaacca acctaaagga gtgatcatgg ataaaagtaa atgtaattca gcatgctctg
    25741 gaacccagga ttgaataaat atcttgcata agccaggatt aactccacaa gctaaccagt
    25801 caataatcat atcccaaagg tgtttttcaa taaaattagg ggattcatat tgtgttgtta
    25861 ggccatgcca atctgctgca aaaaagaaac aatcgtattg atgttgtaat tttatccaat
    25921 tttttaaaac accatgataa tgacctaaat gcaatcttcc actgacccgc attccagaaa
    25981 cgactcgctt attggaagta aacaatgctg acatcaattt cctcttcttg ttataaatta
    26041 atcaattatt tcaaatacaa aaatttcttt attcccagaa ttctaacctt atgcttgctt
    26101 gagtcacatt ttatctagat tagagacatg aaattaataa tcaagaacta attggacaaa
    26161 ataaaattca atggtgtcaa ctctagcttg gtaggggtac ataaaattct ctttcactaa
    26221 atactaacaa aactgtggct gcatgccatt atctaaaggg ttcaggatct ccttttccct
    26281 ctcttataat taccggataa tctccagtta aatctactac tgtagtgggt tcatgaccac
    26341 agtttccacc atcaattatt aaatcaattt ggttgcctaa taaatcttta attgcttcgg
    26401 gctcgcttaa tggcgcttca gcccctggta aaattaaagt ggtgctcatt aaaggggctt
    26461 ccagacactc caataaagac aaagtaatcg tgttatcagg tactcttaac cccaatgttc
    26521 ttcgttttgg gtgaagcatc aaacgaggta cttcatgtgt agcattaaga ataaaggtat
    26581 aagagcctgg agtaaaagcc ttcaataatc taaaaattgg attagaaacc tttgcataag
    26641 tacctaactg ggataagtca cgacaaacca gggtcatatt atgatgctta tccaattgac
    26701 gtaagcggcg aatacgctcc agggctgatt tattgcccaa actacagccc aaagcatagc
    26761 cagaatctgt cgggtaaacg ataagccccc cttcttcaat aattgaaact gcttttctca
    26821 acaagcgtgc ttgaggatta tctggatgta aagcaaaaaa ctgactcata aagtcccctg
    26881 tttcatgttc cattcttgcc aaataggcgt gcatattatt ggtaattgtc gctgacgccc
    26941 cagatttata caagattgag cgtcactatg gtaatctgaa cctgatgagg caagcaaatg
    27001 aaatcgctca cacatcccgg ccatttcttg aatttgtgca gttgtcattt ctccagaaac
    27061 gacttccatg ccaacacctc cagcctcttt aaactccgtg attaactcac gcaattttga
    27121 acgcgtcaat ccatatttaa gtggatgagc aattacagcc tgtccaccag aatgagatat
    27181 acctttgacc gcatcgtgca tgctaatcca taaggtaggt atataggcgc acttgcctct
    27241 cccgaggtaa tttttaaaag ccgacttcat atcttttgcc ttcccttcat taatcagtac
    27301 ttgggcaaaa tggggtcgac ccacccgttt gtggcctgcc aaaagacagg ctttttcata
    27361 agctttgtca ataccaatcg aatgcaagga ttctccaatt ttctcggcac gctctattcg
    27421 acttttgttt tgatgagcaa ttaactcttg taattctgct gtgtgattaa tttggtaacc
    27481 aagtatatga atgtcatact tcttccaacg agtactgagt tctatccctt taactatttc
    27541 aatgtttgta tggattgctg cgtcatataa agcgtcatat cctgcaatag tatcatgatc
    27601 agtaagagat aggcattgaa cttgtaaagt actcgctctt tttattagtt cttctggagt
    27661 cagtgcaccg tctgaaaaag aactatgaca atgaagatcg atcataaatc gcagctaatt
    27721 taaaaaaatc gaagtatagc ataattaacg tgagttcgac ataaaaggct tttatgactt
    27781 ttatgtcgct aaacgcacgt tatcccgtaa aaaagcaaag ctttttatac gggatccatt
    27841 caaaataccc aattctatat aagaaaaacc aggtaaaaat atgatgttgt aaagactaaa
    27901 ctatcctttt ctttgaacaa cttgcacaac atagtacttt tgcactattc tttaagtatg
    27961 gtcattaatc tttaataaaa actttgagta gtacattaaa cagtgaaata atcaaattac
    28021 cacctcttaa taccagggag ctcgcaaacc tgtgtggggt tttgcacagt aacttaaaaa
    28081 ttttagaaca gcacttcaag gtttcaataa agatcaaaaa tcataacatg ataatatgtg
    28141 gccaatcagg tgaccatttt gaggagataa aaaaagtatt aaaattattg tatcctttaa
    28201 ctggcgaacc tatagatacc aaaactctac gagtcttaat caatgaggat tatcaaacag
    28261 caatgactca ccatatcaga ttatctcgaa aatcaatttc tgcacgtaac agcaaacaag
    28321 ccgcatatct ggattctatt aatacgcatg atattacatt tgccgtaggg ccggctggga
    28381 caggtaaaac ctacctggct gtttccaaag caatagaatg ttttgagaaa ggcgaagtac
    28441 aaagacttat ttttgtaagg cctgctgtag aagcaggtga aaaactcggt tttcttcccg
    28501 gcgatctggt agaaaaagtc ttaccttatt taaggcctat ctatgacgca ctgtatgaaa
    28561 tgataggctt taaagaaact caaaagctga ttcaaacaga tatcattgaa attctcccat
    28621 tagctttcat gaggggtaga actttaaatg aatcttttat tattctggat gaggctcaaa
    28681 acaccaccat catgcaaatg aaaatgtttt tgacacgcat gggctttggc tctaaaacag
    28741 tcatcacagg tgatatcact caagtcgact tgcctaaagg tattgactcg ggccttgcac
    28801 atgccattca gttattcaga cattttcctg atatcagcat tcatacattt accagtcgtg
    28861 aagtcgtcag acatcctttg gtttcaaaaa tcgtagattg ctatgataat gagatggaaa
    28921 ggattaaaaa atgacctatc acattgatat acaaaatgct acagggaaat tattgccctt
    28981 gagtgaagat gaaatcacca agctggcatc gctagctcta agagatcata agcaggatgc
    29041 tgagctaaca gttcgtctgg ttgatgttga agaaatgact tatcttaacc atacctatag
    29101 aaagaagaac aaaccaacca atgttcttgc atttccttgc tcactaccgg cgaacattga
    29161 actggaatgt cctcttttgg gtgacgtcgt tatttgccct gaagtgttgc tcgcagaaag
    29221 cgctcaattc aataaatcgc tacatgcaca ttggtcttta atcctgattc atggagtttt
    29281 gcatttatta gggtatgatc atataaaaga tgaagaagcc tctattatgc aaatgctcga
    29341 agctaaatta ctagctgaat taggttatgc taatccatat gaagtcgagg aaaatgaact
    29401 tgaataaatt ggaagatggt gggtcgtggt tttctcgttt aaaacagttc ttacaaggag
    29461 agccacaaaa tcaggaagaa ttggtcagtt tattaagaga cgcgcaaata cgctccctga
    29521 ttaattcaga gaccctcgca atgattgagg gagctatcct tttctcaaaa atgcgtgttc
    29581 gagacatcat gctgcctaaa aatcaaatgg tttgcattaa taaggatgat gattttaagc
    29641 atattatatc tattgtaact caaacgggac attcacgttt tcctgtaacg agtgaaaatt
    29701 ctgatgaagt ggttggcatt ttgcacgcaa aggatttgtt aaaatatcaa ccagaaaata
    29761 tggaatcatt tgatttattg gacatctgcc gccaggtaac ttttgtacct gaaagcagac
    29821 gtcttgatag tctgcttagt gaatttcgaa gtaatagaaa tcatatggct attgttgttg
    29881 atgaatatgg agaggtgtca ggtttcgtta ccattgaaga tatcattgaa caaataatcg
    29941 gtgacataga agatgaattt gatatcgatg aagacgccta tattaaagca catgaaggac
    30001 actgctatat catcaaagcc catactccaa tagaagaatt taacgaacag ctcaacgctg
    30061 attttagtga cgaaacctat gatacaattg gtggcatagt attaaataaa ttcggctatt
    30121 tacctcaacg aggtgaagta ataatcatag atgattttga atttaaagtt cttaatgccg
    30181 attcgagaag aataaagtta ttagaatgtc tcgataaaag aagtcccgag aaactaaatt
    30241 ctactgttga aggataagta attcatgagc agctctattc tcattattga tgatgacaca
    30301 gaactaacag atttattaac ccaatatttg gagccagaag gctttaatgt agtatgtgtg
    30361 catgatggag aaaatggtgt taaaaaagct ttaaatcagg tatttgatgc cattatactc
    30421 gacgtcatgc tgcccaagct taatggtttt gaagtattaa aagcaataag agagcatttg
    30481 gaaacacctg tattgatgtt aactgctcgt ggtgatgata tcgatcgcat tgttgggtta
    30541 gaaattggtg cagatgatta cttacctaaa ccttgtaatc cacgagagtt agttgcacgt
    30601 ctgcgtgcta ttttaagacg cactcaaaaa atcccaactc ccaaaccaat tattgaacaa
    30661 cataatatca ttgtggattg ctccaaacgc cacgtgacta tgggaggaaa attccttgaa
    30721 ttaaccaatg cggaatttaa tattttagag atgttaatca aatcaccagg acaagcattt
    30781 tccaaagagg aactcaccga atatgctctg ggaagaaaat acactgctta tgaccgcagt
    30841 atcgatgttc acatcagcaa tttacgaaat aaattaggcg ataatcctca gggagaacct
    30901 ttagtcaaaa ctgtccgcgg gtttgggtac atgtttaatg cgtagcctgt actggaaaat
    30961 atttgtttct ttttggctgg ccaccatttt aattattttt actacggcat gggttaccag
    31021 ccaaatcgca caaaaatcat cacaaccagc tcgagaagaa atgtttatgg acagctatgc
    31081 caatgcagct gtcgcaacct atgagtcagg ccaacaatca gcacttttga aatggcttga
    31141 taaaataggc ctttctcgtc atatgacact gtatttacta accagcaccg gtgaaatcat
    31201 tggcgcacag aaaccacctg acgccgtaaa aaaaatttca gaaaatttaa taaatgatga
    31261 actcccaagt gatggcatat ttaaaacagg gaaattagtc gttagccatg agatcctatc
    31321 aacttctgga aaatattacc ggctagcagc tgtcagtgaa aagccaatct cttattttgt
    31381 gcaaattcca tgggctggac tgacaatacg tttaactctg gccatattca tcagtgggct
    31441 tatctgctat ttactctcac gttatttaac tcaaccttta cgctcattag gcatggcagc
    31501 aaaatctatc gctaaaggaa atttaaatac ccgggttggt cgcttcagag gtcatagcaa
    31561 agatgagatt gcggagttaa gcaatgaatt cgatcgaatg gccgagcaat tagaagcgct
    31621 tgttcattcc aaagaacgat taatacagga tatttcccat gaattacgct ctccattggc
    31681 tcgacttaaa atcgccatag aattggggaa aaagaaaacc aagcatttgg ctgataatga
    31741 atttgcccgc atggaaattg aaacatccag acttaatgat ttgataggcg aaattctcga
    31801 ttttgctcgt ttagaaaaat cgacaacaga acttcattta tcccaaacaa atatcgcgga
    31861 tttgcttgca caggttataa gtgatgccaa ttatgagttc agtcacaatt ctataagaat
    31921 acaagctgga ataattacac cttgtgaatt aaatattgac caacgcctaa tacacagagc
    31981 tatagaaaat attgttcgta atgcatcaca ctattctcct ccagatgaaa aggtcctgat
    32041 ttcatcaagc tataataaaa acaaagatca agtttatatt gatattaatg ataaaggacc
    32101 tggggttccc gaagatcaac tggaaaaaat atttaatcct ttctaccgcg tggatacctc
    32161 cagaacaaaa aaaaccggtg gctatgggct tgggctcgca attgcagcca gggctgtagc
    32221 attacaccaa ggagaaatta ttgctaaaaa tagagagcaa ggtggtttgc ttgttcgtat
    32281 cattttacct gctaatgtta aatagaattt ctatatatcg agttcctcct tattgactaa
    32341 aattgatttt taacttgcat ttttattatc taatttcgca taaatacaaa
//
LOCUS       DATNSN010000019        32123 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.019, whole genome shotgun sequence.
ACCESSION   DATNSN010000019 DATNSN010000000
VERSION     DATNSN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32123
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.019"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(29..1576)
                     /locus_tag="VAH76_000717"
     CDS             complement(29..1576)
                     /locus_tag="VAH76_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HEO1385365.1"
                     /translation="MVLRYPDIHGIELMSAKWRWYDSLFILLFAYLLVLQIQAIWLFT
                     IDDMYISLRYAKNWAAGNGLLWNLNAEPVEGYSNFSFVALGAISIILHINPVLTLKIA
                     GFAGLLATCLFIFLISRFWFTKRESFIPCIWLLLYKGQVIWSVSGLETTVYQALISGS
                     VYFAFRGLGYHFYPAPRGEFKTSGFVLSGLYLSFAGMTRPETPALMLLFFLLIGWDWN
                     KLSGKKPWRGAVSFAVTIGLVFLPYFLWRWSYYGLLFPNSVYCKGFNGISYSLDLNYL
                     KLIWPFAILALPACFYAQDRRHYFLWSPGIIYLLMLVNADPIVAFDNRLFLPAFALML
                     PLTLNGISYLLLSYFKKQDNYFRAALYVSSFFMALLFIPKMSLDAYRYFSQNPVHGEQ
                     LRKSVVQWLNTHAKNQDRVVLADSGLIPFLSGLTFIDSYCLNNKEMAQFPAVKRYELF
                     CQQILEEKPQIIILTALIDKGKIIYTPGDSCLKPLLDVHKEYKLGGVFSFGSAESQYR
                     YELFANF"
     gene            complement(1537..2421)
                     /locus_tag="VAH76_000718"
     CDS             complement(1537..2421)
                     /locus_tag="VAH76_000718"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEO1385366.1"
                     /translation="MMELANKIIRVSYMDKLYQYLLLLRLSHWSKAIFVMLGFIYTPV
                     PGYLFPALMASLSFCLIASAVYIYNDIQDRTEDSLHPFKCNRPIASEQVTLFDAIIVM
                     LLLMVAGIALGWLISKKLVVILIIYLIINLAYNHVLKLIPVFDVACIALGFMLRVLAG
                     TIGIDLAISAWLTVAATLLSLFIALNKRQLEMQLGLKNSTRKVLRRYNPVFLQRLIVI
                     TGIACFITYLFYTVYARAESFFFILTLPFAAIALWRFAWLATQDVENDDPVNIFLSDR
                     LSRINLWCFAILTFMALN"
     gene            2751..3992
                     /locus_tag="VAH76_000719"
     CDS             2751..3992
                     /locus_tag="VAH76_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0963 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385367.1"
                     /translation="MRYILPAGALTSLNFLRDDYQSERKVDLDQPPSLIGVPTLFGGT
                     DVIARDKQTKFIKKMIVVLRANLAKEEEITTPEQWEANLTASRVVIAACLYVQSQISS
                     PKNNSALYRLINKDLGITDTNYLDDEDKEICFLAANRVVNSSLSAFSDANAALRKANM
                     KPFTEQEWSEFSKFVSSVSVKKVSTNPYSNYPVTSITQPLFGAAFSYTGATIGYLGGD
                     VISQSSKAMSTKLQITTMVGSSLLMLGTTGPMGVALFSQIIASKLVTAFFSISLAHVL
                     GTTMGILGQGVGMGIGLPLDYAYRLIWKASATIMNYYSNYPNTSQMTGIRIADGKSIM
                     NGIVIEYTPSSDLKTEEGQQTVEITEEGKLLINGKEVTIPDGGVQLPLELINELKAQL
                     KLQEEKVSEEDSSSSTLQATI"
     gene            complement(4078..7524)
                     /gene="dnaE"
                     /locus_tag="VAH76_000720"
     CDS             complement(4078..7524)
                     /gene="dnaE"
                     /locus_tag="VAH76_000720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO1385368.1"
                     /translation="MRQRFVHLRVHTEFSLVDGLVQIKPLMKALPARGMSTVAVTDFC
                     NLFAAVKVFKSATEAGIKPIIGSDLPCHDLETPDNIFSLVLLCQDTAGYKNLTCLVSK
                     AYQEGQYHGQPRVQLQWIPEFSRGLIALSGGKFGDIGQALLANDDTLARKRALHWMNL
                     FPNRFYLEIQRTGRADELHYNEKLIALADELQLPLVATNDVRFIDEDDFDAHEARVCI
                     HEGYTLADPRRVQQYSSQQYLRSADEMELLFSDLPSALQNTVEISKRCNVKLELGHNY
                     LPNFPIPDGTTVEAYLSHLSEVGLEERLKQLFRDKSEGELLAARGEYDKRLKIELDVI
                     NNMGFAGYFLIVADFIQWAKQNGVPVGPGRGSGAGSLVAYALKITDLDPLEYELLFER
                     FLNPERVSMPDFDIDFCMEGRDRVIEYVAEKYGRQSVSQIITFGTMAAKAVVRDVGRV
                     LGHPYGFVDKLAKLIPFELGITLSKAMEQEEELRRRYEEEEEVKELFDLALKLEGITR
                     NAGKHAGGVVIAPSQLTDFTAIYCEEGSTQIVSQFDKDDVEAVGLVKFDFLGLRTLTI
                     IDWALATVNKQCENAGLSPIDISQIPTSDDSTFELLKACKTTAVFQLESRGMKELISR
                     LQPDCFEDIIALVALFRPGPLQSGMVDDFIDRKHGRARVDYPHPDLEPILKPTYGVIL
                     YQEQVMQIAQVLANYTLGAADLLRRAMGKKKPEEMAKQREIFTQGAVARGVDEQVATH
                     IFDLMEKFAGYGFNKSHSAAYALVAYQTAWLKAHYPAAFMAAVMSSDMDNTDKVVTFI
                     DECAHMKLQVLPPSINNSMYHFTVSDEKTIIYGLGAIKGVGESAIECIIQERNQNGPF
                     LDLFGFCQRLDLRKVNRRVIEALIKSGAFDVWKVERAVLTASLEKALKVAEKEHQNQS
                     SGQFDLFSLLEDKADEQNYVESKSWSEAQRLEGEREVLGFYLTGHPADQYRRELGDFI
                     VPVSQLNPSLHKKAIICAQVVAIRKIITKRGKKLVIIGMDDATARIDAVVFGEIFEQY
                     SDPLATGNMLVIEGEVAIDDYNGGVKMTANQLLSIASARTKFARCLELRLNQNNAAVL
                     PSIQSLLKTYSGRCAVQLAYANGVAKANLNLGQQWCVTPSDELIGLLSNLLGEEQVIM
                     RY"
     gene            complement(7599..8945)
                     /locus_tag="VAH76_000721"
     CDS             complement(7599..8945)
                     /locus_tag="VAH76_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385369.1"
                     /translation="MTFELLWTDKCFLTLLAVVLFTVLLSLRKQHIKLSFAIILQRPL
                     AISAGIVLLLFLAIGVLDSIHIKFINDKTEKTQSLLDRVLAPIGSTYEKTYSAPLALN
                     QFMSETKLINGVITQVYPRLKYPPASIKNEKDKSLIIKSVLMKGITYSALVILGGWLL
                     LALIKFSLGYKNIFILNSTQISAIATVAVLTFAVVIGYGLSRSFHVLGTGQIGQDIFY
                     FSLKSVRTGLIIGFLTTLFMLPLALFLGIAAGYFGGLVDDIIQYVYTTLSSIPGVLLI
                     TASVLSLQTYIANHPEQFTTLAKSADARLLALCLILGVTSWTSLCRLLRAETLKLREI
                     DYVLAARALGSSWFTIVRKHLVPNVMHIVIITLVLDFSFLVLAEAVLTYVGVGVSPMT
                     ISWGNMINGARLELARDPVVWWPMFAAFLLMFLLVLAMNLFADAVRDAFDPHQSHL"
     gene            complement(8969..9946)
                     /locus_tag="VAH76_000722"
     CDS             complement(8969..9946)
                     /locus_tag="VAH76_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385370.1"
                     /translation="MIKYILRRILYAIPILFGINLITFALFFMVNSPDDMARMHLGNK
                     HVEQKAIEDWKAVHGYDLPLFYNEKQTGFNQFRQTLFYQKSLMLFTFNFGVSDAGRDI
                     SHDISYRMWPSLAIALPTLLFAVLVNIIFAMAMAFFRSTYLDLSGVVICIILMSISSL
                     FYIIGGQYLFGKILKWFPISGYDDGLDALKFTILPVLVAVLGQLGAGARWYRTLVLEE
                     INKDYVKTARAKGLSEQRILFKHVLKNAMLPILTGIVVIIPTLFMGSLVLESFFGVPG
                     LGSYIIDAIQQQDFAIVRAMVFLGSVLYIIGLVLTDFSYILVDPRVRLS"
     gene            complement(9943..12108)
                     /locus_tag="VAH76_000723"
     CDS             complement(9943..12108)
                     /locus_tag="VAH76_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO1385371.1"
                     /translation="MQFMLFLMPCCVNLAYGSSWVFNNPYPESEANENIYYSSFNEQP
                     KTLDPARSYSSNEYLFIAQIYEPLLEYDYYKRPYELIPLTATRLPQIRYLDAQGNPVS
                     AGDNTKPAYSVYTIQIKKGILYQPHPALAQKKDGSYRYHHLPEDYLEEHDISKLSDFK
                     YTGTRELIIDDYIYQIKRLANPAVSSPIYGLMSYYILGFKEYGDSLPKGLANKNSFID
                     LRKYPLKGVKKLDDYTFEITLKGQYTQFLFWLAMPFFAPVPWEADLFYSQKDMDDKNL
                     SFGWYPIGTGPFMLTENNPNRSMILEKNPNFREVYFPVNGSDADRRAGYLTNQGRRLP
                     LIDKAVYTLEKESIPRWNKFLQGYYDMSGISADSFDQAIHINRFGEPILSQEMKDKKI
                     YLRQTLEPSIYYTGFNMLDSVVGGNSKRARKLRQAISIAVNFDENIAIFFNGRGIPAQ
                     GPIPPGIFGYKEGKEGINPYVYQWVNNARKRKSLKDAQKLMVEAGYPGGIDPKTGKHL
                     ILHYDVMTTGGPDDKARLDWMRKQFANIGIDLNIRATLYNRFQEKMRIGHAQIFSWGW
                     NADYPDPENFLFLLYGKNGKVKYGGENASNYENPEFDRLFNLMKNRPNDLQRQKLIDK
                     MLAMVRYDAPWVWGMHSEDFILSQDWVSNIKSNTISLNTLKYVSVNVPERNQLRRSWN
                     QPVFWPLGVLFGLLIVLVIPLLITYLKKEKQPAERVKLK"
     gene            complement(12228..14249)
                     /gene="ligA"
                     /locus_tag="VAH76_000724"
     CDS             complement(12228..14249)
                     /gene="ligA"
                     /locus_tag="VAH76_000724"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO1385372.1"
                     /translation="MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKAL
                     QQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQAMLSLSNVFTTDELKAFIKRAIE
                     KLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
                     RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEIS
                     ASRPLAIYCYGIGACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYM
                     LAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQV
                     GRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIHIGDTVIIRRAGDVIPEVVS
                     VILEKRPANARKIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRK
                     AMYIEGLGSVLIDQLVDEGIVHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKK
                     TTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELMTLNDIGPVAASHIVHF
                     FAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLL
                     ALGAKVSGSVSSKTDYLVAGSEAGSKLVKATELGVAIIEEDEFLKWVNS"
     gene            14471..15370
                     /locus_tag="VAH76_000725"
     CDS             14471..15370
                     /locus_tag="VAH76_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385373.1"
                     /translation="MELLRILSTLIILLFSTLIHSHSDTNSNQGIAFVHGTRDHREDA
                     YGGYWKIDFIQSISQTLAKPENHYVVHCDFSQYMWHEDAANCVANQLLEFINDKNVSS
                     LTVYTHSDGANIMRWILSNPTYDSRYMTLKRKISQVIAIAPSSGGTPLADEVLNGGIF
                     ETSLAWLLGYLSDAVKQQRVGDMLIYNEELLLGSKGRPSLSIPFKVIVGTDVYASPFS
                     TSSYCNGYMLNSGLKIAKLYLNQCADGFLNCDSQAQAGEVWFYDIDKTDYNLPLSHNQ
                     SRHSCFGLDQILISALVNEGVAK"
     gene            15367..16545
                     /locus_tag="VAH76_000726"
     CDS             15367..16545
                     /locus_tag="VAH76_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 family protein"
                     /protein_id="HEO1385374.1"
                     /translation="MKTAHLIWMSVFCIAQAHAFTLPRQPTKSYECENCSQLSRENLH
                     DKWEIINEPLNNNISNARRSYGYKERISLEQLQRGVIISTLAPGAVVRITPLQNKSIP
                     ELLIKTPKSQLLPLKEASSLYNQDDEVGNNPLAITKHQAMLQIKPELGYGKFILKSKD
                     ITDKYADAYMISVLDKFSITYLEVETDSLHYQYGDKLKATISLHNDITEYDVNDVDAR
                     LVGPKGQVISLNLTKLKSNVFEGTATLDSELNDRGENWYLETDVQTEYGQEIIRRSGH
                     TAFSYSIPSASLMNVKKLSSKPLTFVVTVDVATASRYALQSVLFQKNGKGEARPIQTS
                     QRAQWLEPGKHVLQFTFDNHNQLSDDNLYLGYLRLIDYGQLKTVYQYNQPVKLSQLVD
                     "
     gene            16549..17811
                     /locus_tag="VAH76_000727"
     CDS             16549..17811
                     /locus_tag="VAH76_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO1385375.1"
                     /translation="MALFSSVTLFEGIALAIAFQFLLLFWVLVKQAQQKKLTEQLGER
                     IQQTLISQLHQLHIDLSQSYQTSQQFINEKIAQGQLATQQLISDAIQRQMADVREQMT
                     HSFKHHASSLTSHLQLLTEEIRNHLNTLTQQVNHKLTEGFEKTSSTFIDVVKRLTIID
                     EAQKKITELSNHVVSLQDVLVDKKARGAFGEVQLSTLISNMLPSASYQMQYTLSNQKR
                     ADCVLFLPEPTGNIVIDAKFPLETYQRLINSDALSTDRKSLQQQFRQDIQKHIKDIAE
                     KYIVPNETTDGAMMFIPAESIFAEIHANYPDLIAMSQKLKVWLVSPSTLMAVLTTARA
                     VLKDDATRKQVHIIQKHLQALANDFQRFEKRMDKLSKHIDLAHQDVNEVNTSAKKITS
                     RFQKIESVDIELDEHEVTQLELVESESI"
     gene            17888..21349
                     /gene="mfd"
                     /locus_tag="VAH76_000728"
     CDS             17888..21349
                     /gene="mfd"
                     /locus_tag="VAH76_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO1385376.1"
                     /translation="MNTKTLLYQPTKNKQIWGQLYGSSLALALAEYCQQTPGIKLLIT
                     QDNLCASQLQDELQFFLNPNQNPQELLFFPDWETLPYDQFSPHQDIISERLYTLSRIQ
                     QITNGIIISSASTLMHRLSPPEFLNQYALMLKEGQKLDLNSFRNQLQQAGYHCVNKVL
                     EHGEYALRGSIIDVYPMGSGLPFRIELFDDEIESLREFDPETQRTIEKINQIHLLPAR
                     EFPLNEQSQLHFRRAFRESFPGNPSHCPIYEAISNSQFPSGIEYYLPLFFDKTVTFFD
                     YLPANAKICLIEEIQENAERFWRELNERYEQRRHDVSRPILTPDNCFINPTELLTKAN
                     NYEQVRLYHKPSEKKGALNFNIAKAPQLPIDRKNIEPLRQLSEYCSDPTRRYLIVVES
                     AGRREVLLDLLKSSHIIPRTQHSWLDFIHDESPINITTGPLIYGCELTDKQIVIIVES
                     QLFGEQSTPQRRSTQKSVDPDLIIRDMAELRLGAPVVHLQFGVGRYQGLQYIDSNGTP
                     SEFLVLAYAGDDKIYVPVTSLHMISRYTGVDSEHAPLHKLGSDQWQKEKKKAAEKIHD
                     VAIELLDLYAKREAQPGHQYDFNQSEYIKFASGFPFTETPDQLQAIEQIIRDMQSPRP
                     MDRLICGDVGFGKTEVAMRAAFVAVQNGKQVCILAPTTLLAGQHFESFRDRFAEFPVN
                     IELLSRFRSNKESEAVLAALQSGKVDIVIGTHKLFQNNISFKNLGLLIIDEEHRFGVK
                     QKEHIKSLRTHVDILSMTATPIPRTLNMAMAGIRDISLIATPPAKRLAIKTFWQEKND
                     LTIREAILREILRGGQVFYLHNNVQTIERVCQDLEALVPEAKIQSAHGQMRERQLERI
                     MSDFYHHRFNVLVCTTIIETGIDIPTANTIIIDRADKFGLAQLHQLRGRVGRSHHQAY
                     AYLLTPNEKLLTPDAVKRLEAIVSLEDLGAGFTLATHDLEIRGAGELLGEEQSGNMHA
                     IGFNLFMEMLDRAVNDLKAGKTPELSAPMHQGPEIDLRISAIIPEDYIGDIHNRLIMY
                     KRIANAKTTQQLRELQIELIDRFGLLPQPVKHLFLITELKLKAAQLGIQKISASAQQG
                     KLDFSEKPSIDPGTLISLIQVHAKRYQMEGPQRLRFTLDSTSAEERIFEISSLLNKLS
                     PHSQTID"
     gene            complement(21328..22995)
                     /locus_tag="VAH76_000729"
     CDS             complement(21328..22995)
                     /locus_tag="VAH76_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO1385377.1"
                     /translation="MQKESNSNSLFDLLLKNEKNSSDRVYLRQPKNGVWHEYTWSSVM
                     LQARKVAGFLHHLGLKKGDHVSIISKNCAEWFIADFGIHIAGMVNVPLFANQNEESAH
                     YVLEHGDVKLVFVGKLDDHQRVRQFIPKSYPTIGFDYHKDLQVDYEWSDVLKSDPMIN
                     LVEPEPDALYTIIYSSGTSGAPKGAMYTNRSIANYLTVFPKDLLRIRELEHYKLISYL
                     PLAHVYERSAIQLGSVAINASVSFVESLEKFAENLREVSPTFFTAVPRIWGVFKQKIE
                     QKLPPAKLNFLLKIPLISSLIKRKIIHSLGLSECTNCFSGASHLPISIIDFFEKLGIY
                     IQEGYGQSENLAYATLSMLNERKRGYVGTPRLEVEIKVGEENELLMKCPCLMSGYYKN
                     EQATKNAFTEEGWLRTGDVVDVDNEDRVKILGRLSENFKNQTGEFIAPSPIEKLFSVN
                     PMLEQLCLVGRELPSNVLLVTLNEGVRLGMPKEEITHALQETLKQVNSKLVKYEKVSH
                     ILVLKDAWTTENDKLTPTLKVKRRVVEKDYAELIQKALQKSQSIVWE"
     gene            complement(23022..23900)
                     /gene="rssA"
                     /locus_tag="VAH76_000730"
     CDS             complement(23022..23900)
                     /gene="rssA"
                     /locus_tag="VAH76_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEO1385378.1"
                     /translation="MTNQYPKIGLALGSGSARGWAHIGVIQSLKRLGVRIDCVAGCSI
                     GALVGAIYACGTLDLFEQWVLKLNKREMAKLIDVNFLGGGVLSGSKLMTFFKDFGLEK
                     NIEELSLPFSSVATDLSTGREIWLSKGSLELAIRSSMSLPGLFTPQRYKDCWLVDGGL
                     VNPVPITLCRAMGADVVIAVNLNHDLLRVPPSTTEHPGFFSVLSGQFDKMIKRIMGIS
                     DGPGYYDVLTRSIKIMQDRITRARAAGDPADLTLVPQLGNIMLYDFHKAKEAIHEGEN
                     CVLREKNNILQLVGLE"
     gene            complement(24021..25463)
                     /gene="tldD"
                     /locus_tag="VAH76_000731"
     CDS             complement(24021..25463)
                     /gene="tldD"
                     /locus_tag="VAH76_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEO1385379.1"
                     /translation="MTKSLAIAKNILLTPASLDEVGIEKLFKTMFNRNVDDADLYFQS
                     CSYESWYLEDSEVKSGNFSLDKGVGIRAVSGDKTGFAYCDDILLPSMLRAADAARSIA
                     LSGSKTVPAVKVLKASVNRYESLNPLEGMSKQEKIALLEAIDKEARSLDPRVIQVNAT
                     LTGCYEVVMVANMDGQMMADVRPLVSINVSVIVEDSHGRRESARSGGGGRVAYSYFTE
                     KENALSYAREAVREALTNLKAEPAPAGTMPVVLGPGWPGVLLHEAVGHGLEGDFNRKG
                     LSAFSGRLGQQVAAAGVTVVDDGTLENRRGSLTIDDEGTPSQCTTLIDNGILVNYMQD
                     KLNAKLMGMQSTGNCRRESYAHVPMPRMTNTYMLAGQYDPQEIIRTVKKGLYAVNFGG
                     GQVDITSGQFVFSASEAYLIENGKITKPVKGATLIGNGPDVMKKISMIGNDLALDRGI
                     GVCGKEGQSVPVGVGQPTLKIDALTVGGTK"
     gene            complement(25475..26281)
                     /locus_tag="VAH76_000732"
     CDS             complement(25475..26281)
                     /locus_tag="VAH76_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEO1385380.1"
                     /translation="MNRVALVQMVSSAKIADNLQITEQSLIQAREQEASLVVLPENFA
                     FMGMNEREKLHIAEYYGQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYD
                     DKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
                     ELYQYLTQRGAQLFSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRH
                     TYGHSMMVEPWGKVLAQKEEGQGVILADIDLERLSQLRRQFPCVEHHVLM"
     gene            26500..27174
                     /locus_tag="VAH76_000733"
     CDS             26500..27174
                     /locus_tag="VAH76_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO1385381.1"
                     /translation="MNRNDITILSQQRESVLATNKVLRNTYLLLSLTFIFSALTAYVA
                     FISGARPMNPLLMIVGVYGLMFLTQALRNSVWGLVSVFAFTGFLGYTIGPLLNYYITG
                     FSNGPQIVATALGGTGMIFFALSGYALTTKKDFSYLGGFLFVGVMVALLAMIAGIFIQ
                     IPALQLVISAAFVLISSGLILLQTSAIIHEGETNYIMATIGLFVSIYNLFVSLLNLLS
                     AFSGRD"
     gene            complement(27427..27681)
                     /locus_tag="VAH76_000734"
     CDS             complement(27427..27681)
                     /locus_tag="VAH76_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385382.1"
                     /translation="MALIIIHMRGRFDCYLMQFNLHDNIIWQCLASPGIFLVTDRISD
                     CSHQPMAVDGRPGLLCFLGILQIKRHGDYCSLIYSEETVA"
     gene            complement(27672..27947)
                     /locus_tag="VAH76_000735"
     CDS             complement(27672..27947)
                     /locus_tag="VAH76_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385383.1"
                     /translation="MKKYLLFILAGSLFMFTAAQASVNVDAMSQCMKPCNGDKKCLDN
                     CVSNRGIAKDLAQCLIGCGKGIANQTGETSNMDDLKACLRGCVNEWH"
     gene            complement(28491..29459)
                     /locus_tag="VAH76_000736"
     CDS             complement(28491..29459)
                     /locus_tag="VAH76_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEO1385384.1"
                     /translation="MNSNYKREDFANETEVRWCPGCGDYAILAALQRVLPELGLPPEQ
                     HVFVSGIGCAGRLPYYMNTYGFHTIHGRATAVATGLKAMREDLCVWVITGDGDALSIG
                     GNHLLHILRRNVNVNILLFNNQVYGLTKGQFSPTSQKGQVTKTSPKGVTSEPINPIMI
                     ALASGASFVARAVDKDPVHLASVLKKAYEHKGCSFVEIYQDCNIFNHGAFDDFAVKNN
                     RAENTIILEDGQPLVFGAQKEKALQLDDEQFVQISANEKALYKHDAKSLISAMRLARL
                     TFPDYPVPLGVYYQKDRELFTLSQEVKKRPEDLPELFKAKASWKQS"
     gene            complement(29443..31260)
                     /locus_tag="VAH76_000737"
     CDS             complement(29443..31260)
                     /locus_tag="VAH76_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEO1385385.1"
                     /translation="MSTTDVIVVRITGDSGDGVQLVGEQLTISAALTGRDVRTMPDFP
                     AEIRAPAGTVAGVSGFQLAMAERAIFTAGESLDVLVALNPAALKNSLQHLNQGGLLII
                     NEDSFQERDWQKAGIDKSFLDNCREHYHIVAFPLITQTIQAVESINLTKPQAVKTKNF
                     YILGLVLWLFDLPTETCQAFIAKKFKTNQAIAKANELALLAGYNYAMTLELSRRDYML
                     GEVNRHSGEYRQITGVEAVGLALATLATQTQVPLLVSGYPITPASAILHECVRLSDYG
                     VQLIQAEDEIAAICSCIGAAYGGRLALTCTSGPGLDLKCESLGLAVVTELPLVLVDVQ
                     RAGASTGLPTKTSQSDLKQALYGRHGEAPLPVIAAQSPADCFNTIIEAFRIAIKYMTP
                     VIVLLDAYLANAAEPWEIPALDSLNIPKPEFNRFTKPYHRDEFLSRSWNVPGTSGFIH
                     QIGGLEKQGDEGRVSYDAENHQKMVITRAEKIKGIARDYPALVLEGDMTAPTLVIGWG
                     STFGSLKSAVHQCLEQGLPVAYLHLRHLNPLPDDLGSLLKKYDKVLVAELNSGQLCQL
                     IRAVYLVDAQSISQCNGQPFSVNHLVSAIKAEANYEQQL"
     gene            complement(31247..31993)
                     /gene="pdxJ"
                     /locus_tag="VAH76_000738"
     CDS             complement(31247..31993)
                     /gene="pdxJ"
                     /locus_tag="VAH76_000738"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEO1385386.1"
                     /translation="MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGIT
                     LHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAMLDFAEEISPEHACLVPEKREEL
                     TTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
                     ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHA
                     IIARALFCGLKEAVRHMRQLMQEARLYVND"
BASE COUNT        10095 a         7189 c         5583 g         9256 t
ORIGIN      
        1 atggtacact atgttacagt ttcccgcatc aaaaattagc aaacaattca tacctatatt
       61 gtgactcagc actgccaaaa gaaaaaacac cacccaactt gtattcttta tgaacatcaa
      121 gcaatggttt caaacaagaa tcaccgggag tataaatgat ctttccttta tcaataagcg
      181 cagtcaaaat aattatctgt ggtttttctt ctaaaatctg ctggcaaaat aattcatatc
      241 tcttcacggc aggaaattgt gccatttctt tattattcag gcagtaagaa tcaataaaag
      301 ttaatccgct taaaaacgga atcaaaccag aatctgccaa caccactcta tcttgattct
      361 tggcatgagt atttaaccat tggacaacac ttttccgtaa ttgctctcca tgtaccggat
      421 tttgactaaa gtagcgataa gcatcaaggg acattttggg tataaatagt agcgccataa
      481 aaaatgaact cacatacaat gccgctctaa agtaattatc ttgttttttg aaatagctta
      541 ataaaagata actgatacca ttcaaggtca gaggtaacat aagagcaaac gctggtaaga
      601 acaatcgatt atcaaaagcg acaattggat cggcattcac aagcattaat aaataaataa
      661 taccaggaga ccataaaaaa taatgccttc tgtcctgagc ataaaaacag gctggcaaag
      721 ccagaatagc aaatggccaa attaatttga gataattcaa atccaatgaa taggatatgc
      781 cattaaaacc tttacaatat acagagttag gaaacaataa cccgtaataa ctccaacgcc
      841 aaagaaaata aggcagaaac accaaaccta tggtcaccgc aaaagatacc gcccctctcc
      901 atggcttctt gcctgataat ttattccaat cccaaccgat taaaagaaaa aagagaagca
      961 tcagggctgg tgtttcaggc ctggtcattc ctgcgaatga aagatacaac ccggacaaga
     1021 caaaaccact tgtcttaaat tcacctcgag gagcgggata aaaatgatat cctaacccgc
     1081 gaaaagcaaa atagactgaa ccgctaatca gtgcctggta aacggtcgtt tcaaggccgc
     1141 taactgacca aataacttgt cctttgtata gtaaaagcca aatgcagggg ataaaagact
     1201 ctcgtttggt aaaccaaaag cggctgataa gaaagataaa aagacacgtg gcaagtaatc
     1261 cagcaaaccc ggcgattttt agggtaagaa ccggattaat gtgcagaata attgatattg
     1321 cccccaaagc aacaaagctg aaattggaat aaccttcgac cggctcagcg tttaaattcc
     1381 ataataagcc atttcctgca gcccagtttt tggcataccg caaagaaata tacatgtcat
     1441 ctatagtgaa cagccatatg gcctggattt gtaaaaccag caaataagca aacaaaagga
     1501 taaacaggct gtcataccag cgccacttag cactcatcaa ttcaatgcca tgaatgtcag
     1561 gatagcgaag caccataaat tgatccgaga taatcgatca cttaaaaaaa tattgaccgg
     1621 atcatcattc tctacatcct gagtggcaag ccaggcaaaa cgccatagtg caatagcagc
     1681 aaaaggtaag gttaaaatga aaaaaaacga ttctgctctg gcataaaccg tgtaaaacag
     1741 ataagtaata aaacaggcta ttcctgtaat gacgataagt ctttgcagaa agactggatt
     1801 atatcggcgt agaaccttac gagttgaatt tttcaatccc aactgcattt ccagttgtct
     1861 tttatttagg gcgataaata aactaagcaa agtcgctgca acagttaacc aggctgatat
     1921 agccaaatca attcctattg ttccggccaa tactctaagc ataaatccta gggcaataca
     1981 agcgacatca aaaaccggaa tcaattttaa aacatggtta taagcaagat tgattatcaa
     2041 ataaataatt aaaatgacca ccagtttttt tgaaataagc caacccagcg cgattccagc
     2101 aaccatcaat aaaagcatta caatgatcgc atcaaacaaa gtgacctgct cgctggctat
     2161 aggtctgttg catttaaagg gatgcagact gtcttccgtt ctatcctgaa tatcattata
     2221 aatataaacg gcacttgcaa taagacaaaa agatagtgaa gccattaatg ccggaaacaa
     2281 ataccctgga acaggagtat aaatgaaacc gagcataaca aaaattgctt tactccagtg
     2341 cgatagacga agaagtaaaa gatattggta taacttatcc atgtaactta ccctgatgat
     2401 tttatttgct aattccatca tacaaatcta cccttttctc aaaaacagct aaaacagttc
     2461 tgtcatttct aatcaaagta ttaatgaccg cctcaatcag gctacaggac atggatttgt
     2521 tttaatttta aattaattac accctataag agtaacatat ctaactcaag gctaaaaata
     2581 gtataatcgc aaaaaaatca tcatttcaaa gagttaaatc ataaataaaa tctgttgcct
     2641 tatctgattg caacaattta gtaaaaatta accaaatcag attgcattta accagctaat
     2701 cggtaagata aaaaacacac aaataaattt tcaatggata tgaggttatt atgcgctata
     2761 tactgccagc aggagctcta accagcctta actttttacg cgatgactat caatcagaaa
     2821 gaaaagtgga tttggatcaa cctccatctt taatcggagt acctaccctt tttggaggca
     2881 ctgatgtcat agccagagat aaacaaacca aatttataaa aaaaatgatt gttgtcctta
     2941 gagcaaatct ggcaaaagag gaagaaatca caacccctga acaatgggaa gcgaatttaa
     3001 ctgcttctag agtcgtcatc gcagcctgtc tatatgttca atcccaaatt tcatctccca
     3061 aaaataactc cgcattatat cgattgatta ataaggattt aggcattact gataccaatt
     3121 atctggatga tgaagacaag gaaatctgct ttttagctgc caatcgagtg gtcaactcct
     3181 ctttatccgc atttagcgat gccaacgctg ccttgcgcaa agccaatatg aagccattta
     3241 ccgaacagga gtggagtgag ttttccaagt ttgtcagtag tgtgagtgtt aaaaaggtat
     3301 ctaccaatcc ttactcgaac tatcccgtca catccattac tcaaccgtta ttcggagccg
     3361 ctttttctta tacaggagca acgattggtt atcttggggg ggatgttatt agccaatcct
     3421 ccaaagcgat gtcgaccaaa ctacaaatta ctaccatggt gggaagcagc ctgctgatgc
     3481 tcggtacaac agggcctatg ggtgtggcgt tgttctctca gatcatagca agtaaattag
     3541 tcacggcatt tttcagtatt agtttggctc atgttttagg aacaaccatg ggtattctcg
     3601 gacaaggcgt aggaatgggc attgggttac ctttggatta tgcctacaga ttaatatgga
     3661 aagccagcgc cactattatg aattactaca gcaactatcc taatacctca caaatgacgg
     3721 gtattcgtat tgcggatggc aaatccatca tgaatggcat agtgattgaa tacactccaa
     3781 gctctgattt aaaaactgaa gaaggacagc aaacagttga aattactgaa gaaggaaaac
     3841 ttcttatcaa tggaaaagaa gtcactattc ctgacggcgg agttcaactt ccactagagt
     3901 taattaatga attaaaagct caattgaagc tgcaggaaga aaaagttagc gaagaagact
     3961 cttcctcttc cacattgcaa gcaactattt gatgtagatg agacaactat cattctgcaa
     4021 tgcgagttga gctgcgctat ctgctcgact ccacaggcgc tatcattagc agtatttcta
     4081 ataacgcatt attacttgct cttcccctaa aagattgctc aatagtccaa ttaactcatc
     4141 gctaggggtc acacaccatt gttggccaag atttaaatta gctttagcaa caccattagc
     4201 ataagccaat tgaacagcac accgtcctga ataagtcttc agtaaggatt ggatagaagg
     4261 caatacagca gcattgtttt gatttaaacg caactccaga caacgagcga attttgttcg
     4321 agctgaagcg atacttaaaa gctgattggc ggtcatttta acaccaccgt tgtaatcatc
     4381 aattgcaacc tcgccctcaa ttacaagcat gttacccgtc gccaatggat cagaatattg
     4441 ttcaaaaatc tcaccaaaaa ctacggcatc aatcctggca gtcgcatcat ccattccaat
     4501 aatcactaat ttcttgccac gtttggtgat aatttttcta attgctacaa cctgagcgca
     4561 aataattgct tttttatgta aagacggatt taattggctg acaggaacaa taaaatctcc
     4621 caattcacgt ctgtattggt cagcaggatg gccagtgagg taaaaaccta atacctctct
     4681 ttcgccttct aatctttggg cttcagacca agatttactt tccacataat tttgttcatc
     4741 cgctttgtcc tctaaaaggg agaatagatc aaattgccca cttgattgat tttgatgttc
     4801 cttttcggca actttcagtg ctttttccaa ggacgctgtt aatacagccc tttccacctt
     4861 ccaaacatca aaagcaccac tcttgattaa agcttcaatc actcttctgt tgactttgcg
     4921 taaatccaat ctttgacaaa aaccaaacaa atccaaaaaa ggtccattct ggtttctctc
     4981 ctgaataata cactcaatcg cagattcgcc aactcccttt attgccccaa ggccataaat
     5041 aatcgtcttt tcatcggaca cggtaaaatg atacatggag ttattgatag aaggaggcaa
     5101 cacttgtaat ttcatgtgcg cgcattcatc aataaaagtt acaaccttat ccgtgttatc
     5161 catatcagag gacatcaccg cagccataaa agctgcagga taatgtgctt ttagccaggc
     5221 ggtttgataa gcgactaaag cgtacgcggc agaatgtgat ttattgaatc catatccggc
     5281 gaacttctcc attaaatcaa aaatatgggt tgctacctgc tcatctactc ctctcgcaac
     5341 agctccctgg gtaaaaattt ccctttgctt tgccatttct tccggttttt tcttccccat
     5401 ggctcgtctt aacaagtcag cagcgccaag cgtgtaattg gcaagaacct gagcgatttg
     5461 cattacttgt tcttgataga ggataacccc ataagtgggt ttaagaattg gttccaagtc
     5521 tggatggggg taatcaactc gagccctgcc atgcttcctg tcgataaagt catccaccat
     5581 ccctgactgt agaggacctg gtctgaataa agcaaccaaa gcaatgatgt cttcaaaaca
     5641 atcaggttga agccggctaa tcagctcctt catgcctcga gattccagct gaaatacagc
     5701 agtggtttta catgctttta ataactcaaa agtagagtca tcactcgttg gaatttgact
     5761 aatatcaatt ggtgacaacc cggcattttc gcattgctta ttcactgtgg ccagagccca
     5821 atcgattatg gttaatgttc ttaatcctaa aaaatcaaac ttgactaacc caaccgcctc
     5881 aacatcgtct ttatcaaatt ggctaactat ttgtgttgat ccttcttcac agtatatcgc
     5941 tgtgaaatca gtcagctgag aaggtgcaat aactactccc ccagcatgtt tgcctgcatt
     6001 acgagtaatt ccctccagtt tcagggcaag atcaaatagc tcttttactt cctcctcttc
     6061 ttcatatcgc cggcgaagct cttcttcctg ctccatcgct ttacttaaag ttattcctaa
     6121 ctcaaaggga attaatttcg caagcttatc cacaaagcca taaggatgac ctaaaacacg
     6181 cccaacatct ctcaccacag cctttgcggc catagtgcca aaagtaataa tttgggaaac
     6241 gctctgccgg ccatatttct ctgctacata ttcaatgacc cgatcacggc cttccatgca
     6301 aaaatcaata tcaaagtcag gcatggaaac acgttcgggg tttaagaatc gttcaaaaag
     6361 cagctcatac tctaaaggat ccagatctgt tatttttagg gcataagcaa ccagagaacc
     6421 agcaccagaa ccacgacctg gtcctactgg aaccccattt tgtttagccc attgaataaa
     6481 atcagcaaca atcaggaagt aaccagcaaa gcccatgtta ttaataacat ccagttcaat
     6541 tttaaggcgt ttgtcgtact cccctctggc agcaagcagc tccccctcag acttgtctct
     6601 gaataattgc tttaaacgtt cctccaagcc aacttcagaa agatgtgata aatacgcctc
     6661 taccgttgtt ccatccggta tagggaaatt aggtagatag ttatggccca gctcgagttt
     6721 cacattacag cgtttactaa tttcgactgt gttttgtaat gcactgggaa gatccgaaaa
     6781 taatagctcc atttcatctg ctgagcgcag atactgttgg gagctgtatt gttgaactct
     6841 cctgggatca gccaacgtgt acccttcgtg aatgcataca cgagcttcat gagcatcaaa
     6901 atcatcctca tcgatgaatc taacgtcgtt ggttgcaacc aaaggtaatt gcaattcgtc
     6961 cgctaacgcg atcagcttct cattataatg caactcatca gctcttccgg ttcgttgaat
     7021 ttccagataa aaccgattcg gaaataaatt catccaatgc aaagcacgtt ttcttgccaa
     7081 ggtgtcatca tttgccagca atgcttgccc aatgtcaccg aactttcctc cggataatgc
     7141 gattaatccc cgtgaaaatt ctggaatcca ttgtaattgc accctgggtt ggccatgata
     7201 ttgcccttcc tgatatgctt tggataccag acaggttaaa tttttatagc ctgctgtgtc
     7261 ttgacataac aaaaccaaag aaaaaatgtt atccggggtt tccaaatcat ggcatggcaa
     7321 atcactacca ataatgggtt tgattcccgc ctctgtcgca cttttaaata ctttgactgc
     7381 agcgaataaa ttacaaaaat ccgttacagc aactgtgctc atacctctgg ctggcaaagc
     7441 tttcatcaat ggtttaatct gtaccaaacc atcaactaat gaaaattccg tatgtactct
     7501 taaatgaaca aaacgttgtc gcatagagaa ttaacccatt cacgataatt tcgaaacttt
     7561 aacctaaatc ccggaaatag gaaactaaca gatattgact ataaatgaga ttgatgtggg
     7621 tcaaaagcat cccgtactgc gtctgcaaac aagttcattg ccaaaaccaa taaaaacatt
     7681 aacagaaagg ctgcaaacat aggccaccat acaacagggt cacgtgctaa ttcaaggcgc
     7741 gccccattaa tcatatttcc ccaactaata gtcatgggtg aaacccctac ccctacatag
     7801 gtcaatacag cttctgccaa gactaaaaaa ctgaaatcca ataccagggt gatgataaca
     7861 atatgcatga cattgggcac caaatgtttg cgtacgatgg taaaccaact actgcctaag
     7921 gcacgtgcgg ccaggacata atctatttct cgcaatttta aagtttctgc tcgcaaaaga
     7981 cggcacaggc ttgtccaact ggtcacaccc agtatcaagc aaagagctaa taaacgtgca
     8041 tcggcactct tggccaaagt agtaaattgc tctgggtggt ttgcaatata agtttgcaat
     8101 gacaatacgg aagccgtaat tagcaaaaca cctggaatgg agcttaaagt agtataaaca
     8161 tactggataa tatcatccac aagcccacca aaataccctg ctgcaatccc cagaaataaa
     8221 gccagtggca acatgaagag ggttgttaaa aaaccaatga ttagacccgt tcgcacactt
     8281 ttcaaactga aataaaaaat atcttgcccg atttgccctg tgcccaagac atgaaaggat
     8341 cttgataatc cgtaaccaat gacgaccgca aaagtaagta cagctacagt cgctatagcg
     8401 ctgatttgag tagaatttaa aatgaaaata tttttatatc caagactaaa ctttattaaa
     8461 gcaagtaata accacccacc caaaatgact aaagcactat atgttatacc tttcataagc
     8521 acagacttga tgataaggga tttgtccttt tcattcttaa tagaggctgg tggatatttt
     8581 aagcgagggt aaacctgagt tatgactcca ttaatcaact tcgtttcact cataaattga
     8641 tttaaagcca aaggagctga ataagttttc tcataggtac tccctatagg tgcaaggact
     8701 ctatctaata gagactgggt tttttctgtt ttatcattga tgaatttaat atgaatggag
     8761 tccaaaacac cgatggctaa aaacagcaac aacacaattc cagcactgat tgctaacggc
     8821 ctttgtaaaa tgattgcaaa tgacaatttg atgtgttgtt ttctaaggct cagcaaaacg
     8881 gtaaataaaa ccaccgccag taaagtaaga aaacatttgt cagtccaaag caattcaaat
     8941 gtcatttttg ccacatactc cttggttatc aggataatcg cactcgggga tcaactagga
     9001 tgtaagaaaa atcagtcaga accaggccta tgatgtaaag aactgacccc agaaatacca
     9061 tagctcgtac aatagcgaaa tcttgctgtt gtatcgcatc aataatatag ctacccaagc
     9121 ctggcacacc aaaaaaagac tctaaaacca aactccccat aaacaaagta ggaatgatta
     9181 caacaattcc ggtcaaaatg ggtaacatgg cattttttaa tacatgctta aataaaatcc
     9241 tttgctcaga gagccccttt gctcttgcgg tttttacata atctttatta atttcttcca
     9301 aaaccaaggt tcgataccaa cgcgctcccg cccccaattg tcccagaaca gctaccagga
     9361 ctggtaaaat agtaaatttt aaagcatcga ggccatcgtc gtagccggaa atagggaacc
     9421 attttaatat ttttccaaac agatattgcc cgccaatgat gtaaaataaa ctggaaatgg
     9481 acattaggat aatacaaata actaccccgg ataaatcaag ataagtcgaa cggaaaaatg
     9541 ccatagccat agcaaaaatg atattcacca aaacagcaaa taataatgtt ggcaaggcaa
     9601 tagcaaggct tggccacatt ctgtaggata tgtcgtggct aatatctcta ccagcatccg
     9661 agacaccaaa attaaatgta aacagcatca gcgatttttg ataaaacaaa gtctgtctaa
     9721 attgattaaa tccagtctgc ttttcattat aaaataacgg taaatcatag ccatgaaccg
     9781 ctttccaatc ttcaatggct ttttgctcta catgtttatt acccaagtgc atgcgtgcca
     9841 tatcatcggg agaattaacc atgaaaaaca atgcaaaagt aataagatta ataccaaaca
     9901 aaattggaat cgcatataaa attctgcgga gtatgtattt aatcatttta attttactct
     9961 ttctgccggc tgtttttctt tctttaaata agtaatcaat aaagggatta ccaaaacaat
    10021 caataggcca aacagcacac ctaaaggcca aaataccggt tgattccagg atcttcgcag
    10081 ttggttacgt tcaggtacat taacagaaac atattttaga gtattgagag aaatcgtatt
    10141 ggattttata ttagataccc aatcttgcga taaaataaag tcttcagaat gcatacccca
    10201 tacccatgga gcatcatacc ggaccatcgc taacatctta tcaatcagtt tttgtctttg
    10261 tagatcgtta gggcgatttt tcatcaaatt aaacaatcta tcaaattcag gattttcata
    10321 atttgatgca ttttcaccac catacttcac ttttccattt tttccataaa gcaaaaataa
    10381 aaaattttcg ggatcaggat aatcggcatt ccagccccaa ctaaatattt gagcatgacc
    10441 tatccgcatc ttttcctgga aacgattgta aagggtcgcc ctaatattca aatctatccc
    10501 tatatttgca aattgctttc gcatccagtc aagccttgct ttatcatcag gtccacctgt
    10561 cgtcataaca tcataatgaa gaatcaggtg tttcccggtt ttcggatcta tccctcccgg
    10621 atatcctgcc tcaaccatta acttctgcgc atcctttaag gattttcgtt tcctggcatt
    10681 attcacccat tgataaacat agggattaat accctctttt ccttccttgt acccaaagat
    10741 accaggtgga atagggcctt gcgccggaat acctcggcca ttaaaaaata tggcaatatt
    10801 ttcatcaaaa ttcaccgcaa tagaaattgc ctggcgtaat tttcgtgctc ttttgctatt
    10861 gcccccaaca acgctgtcaa gcatattaaa gccagtataa tagatagacg gttccagggt
    10921 ttgtcttaaa tatatttttt tatctttcat ttcctggcta aggataggtt ctccaaagcg
    10981 attaatatga atagcctgat caaaactgtc agcacttatc cccgacatat catagtagcc
    11041 ttgcaagaat ttattccatc gaggtatgga ttccttttca agggtataaa cggctttatc
    11101 aattaagggc aagcgccttc cctgattggt taaataacct gcccgtctat cggcatctga
    11161 accattgaca ggaaaataca cttctctgaa attaggattt ttctccaata tcatgcttct
    11221 attgggatta ttttcagtca gcataaaagg ccctgtacct ataggatacc agccaaaaga
    11281 taagttctta tcatccatat ctttttggga ataaaataag tccgcttccc aaggaaccgg
    11341 agcaaaaaag ggcatggcta accaaaacaa aaattgtgta tattgtccct ttaatgtaat
    11401 ctcaaaggtg taatcatcta attttttaac ccccttcaaa ggatacttac gtaaatcaat
    11461 aaaagaattt ttattggcca atcctttagg aagactatct ccatactcct tgaagcccag
    11521 aatgtaatag ctcatcaatc catatatcgg agaactcaca gcagggtttg ccaatctttt
    11581 aatttgataa atgtaatcat caatgatcag ctcccttgta ccggtgtatt taaaatctga
    11641 caacttgcta atatcatgct cttccaaata atcttctggt aagtgatgat atcgataaga
    11701 gccatctttc ttttgagcca aagcaggatg aggttgatat aaaattcctt tttttatctg
    11761 aatggtatag acactgtatg ctggctttgt gttatctcct gccgaaacag gatttccctg
    11821 ggcatccaaa tatcgaatct gtggtaagcg ggtagctgtt aatgggatta attcataggg
    11881 tcttttatag taatcatatt ccagcagtgg ctcatagatc tgggctatga aaagatattc
    11941 attggaagaa taggaacgtg ccggatccag ggtttttggc tgttcattaa aagaagaata
    12001 ataaatattt tcattggcct cgctctctgg gtaaggatta ttgaataccc aagaactgcc
    12061 ataagccaaa ttgacacagc aaggcattaa aaataacatg aattgcatta acaaatatcg
    12121 ccgcacttac ttccctcgtg ccaatcagtc attattacag gtttaaatac ctacttcacc
    12181 cgattcattg atacccatca taaaccaaat aacaaagatc tagctactca tgaattaacc
    12241 cattttaaaa actcgtcttc ctctataatc gctactccca gttctgtcgc tttaaccagc
    12301 tttgaaccgg cttcacttcc tgctactaaa taatccgttt tggaagacac acttccactc
    12361 acttttgcgc ctaaggctaa taattttgcc tttgcctctt ccctccccat ggtactcaga
    12421 gttccagtta aaactactgt tttaccaaaa aatggatttt gctgattaac ctgtattttt
    12481 tcgggcttag gccaatgaat gcccaactcg agaagacggt caatcacttc aagattatgc
    12541 gcttgggcaa agaaatggac tatatgagaa gccgctactg gacctatatc attcagagtc
    12601 attaattcct caatcgttgc cgctttcaag ctctctacat cgcagtagtg ctccgctaaa
    12661 acccttgcgc cggcttcacc gatttctctg atccccaaag cataaagaaa gcgattgaaa
    12721 gtagtctttt tacttttttc caaagcggat aataggtttt ttgcagattt ctcccccatc
    12781 cttggcaggt tagctaaagt ctgcaaatcg agttcataaa gatccgccaa atgatggaca
    12841 ataccctcat caactaactg atcaattaaa acactgccaa gtccttcaat atacatcgct
    12901 tttcgagaag caaaatgcca catcatcctt tttaattgtg ctttacaata taaaccgccg
    12961 acacaacgag caattgcttc atccgcttcc cttacgactt cagaaccaca aacagggcaa
    13021 tttttaggaa gctcaatctt tctggcatta gcgggacgct tttccaaaat cacagaaacc
    13081 acttcaggga tcacgtcacc agcgcgacga ataataaccg tatcaccaat atgaatgtct
    13141 tttcgggtaa tttcatcaaa attatgcaaa gtcgcattac ttactgtaac accaccaaca
    13201 ctaactggct ccaaacgtgc aaccggcgtc acagcgcccg ttctacccac ctggaaatcc
    13261 acggctatca ggcgagtcat ttcttctgta gcgggaaatt tatgggcaca agcaaatctt
    13321 ggggcacgag aaacataacc taattgctgt tgcaaggaga tactgtcaat cttataaaca
    13381 accccatcta tttcaaatgg caattgattc cgtttcgcta acatatactg gtaataatct
    13441 aaacagcctt ctactccaac cgccctcctc gtttctggag acactctaaa tccaaactct
    13501 tttaataaat tcaattgctc caaatgactg ttaggtaact tataatcctc gcaagcccct
    13561 ataccataac aataaatagc aagtggacga ctggcagaaa tttcaggatt taattgcctt
    13621 aaactgcctg cagcagcatt tcgcggatta gcaaaagttt tttcaccgag ttctctagcc
    13681 cttgcgttgt atgcttcaaa atcggctttg gggatataga cttcaccccg cacttcgatc
    13741 aattttggag ggttactaac ccttagcctt aatggaactg aagcaatagt cttaatattt
    13801 gctgtgatgt tttcacctac agcaccatcg ccacgagttg cggcatgagt caagatcccg
    13861 ccctcataag tcatgttaac agccaaccca tcaagctttg gttcgcaagc aaataccagt
    13921 tgttgatttg gttcatccag tttctcaatt gctcgcttaa tgaatgcttt taattcatcg
    13981 gtagtaaaca cattggacaa agacaacatg gcttgcttat gggctaccgg cataaaagca
    14041 tctgaaggag taccgctcac tctctgtgta ggagaatctg gcgataaaaa ttgcggatat
    14101 tgctcttcat actgttgcaa tgccttgaaa catcgatcat attccgcgtc aggaaccaaa
    14161 ggttcatcta aaacataata gtgataatcg tattttctta tttgctcttt aagcgtttct
    14221 atcgattcct taattccttg atcattcatt aatgaagatt cccaaaataa aaacaacagt
    14281 ttatcatttt tttacttctt tcaaatatga atggtttaat cattcaaggc aaaatctttt
    14341 tttggaagcc tctttattcg gaaccaagta atcggcgcaa atccactaaa acgcatcaat
    14401 cccatctctt gaatcttttg ccagaaccat gatatagata cgtctctgta ttgaaggaga
    14461 tattttgaaa atggaacttc tgcgcatttt atccactctt attattttat tgttttcaac
    14521 ccttatccat tctcattccg acactaattc caatcaaggc attgcttttg tccatggtac
    14581 tcgagatcac agagaagatg cctatggagg gtattggaaa attgatttca tacaaagtat
    14641 ctcccaaacc ctggcaaaac ctgaaaatca ctacgtagtt cattgtgact ttagccaata
    14701 tatgtggcat gaagacgcag ccaattgtgt tgccaatcaa cttctggaat ttattaacga
    14761 taaaaatgtg agctctctca cagtctacac ccactctgat ggtgccaaca tcatgcgttg
    14821 gattctctca aacccaactt atgatagccg atatatgact ctcaaacgca aaatcagtca
    14881 agtgattgcc attgcacctt ccagtggagg gactcctctt gctgatgaag tattaaatgg
    14941 cggcatattt gaaacgagtc tggcctggtt actcggttat ctttcagatg cagtcaaaca
    15001 acaacgtgta ggcgacatgc tgatctataa cgaagaattg ctattgggaa gcaaaggccg
    15061 accctctctg tccattccat ttaaagtcat agtgggtact gatgtctatg cttcaccatt
    15121 ttcaacatcc agctattgta atggatacat gctaaatagc gggctaaaaa ttgccaaact
    15181 ctatcttaat cagtgtgctg atggctttct gaattgtgat tcgcaagctc aagcaggaga
    15241 agtatggttc tatgatatag acaaaactga ttacaatttg cctttaagcc ataatcaaag
    15301 tcgacattct tgttttggtt tggatcaaat acttatcagt gccctagtta atgaaggagt
    15361 tgcaaaatga aaactgccca ccttatatgg atgtccgtat tttgtattgc ccaagctcat
    15421 gcgtttacat taccaagaca accaaccaaa tcttatgaat gtgagaattg cagccaatta
    15481 tcacgagaaa atctgcacga taaatgggaa attattaatg aaccattaaa caacaacatc
    15541 agtaatgctc gtagaagtta tggttacaaa gaacgtattt ctctggaaca attacaacgc
    15601 ggagttatta tatcaacatt ggctcctggc gctgtcgttc gcattacccc tttgcagaat
    15661 aaatccatcc cggaattact tatcaaaacg ccaaaaagcc aactcttgcc cctgaaagaa
    15721 gcctcttctc tctataatca ggatgacgag gttggtaata atcctttagc cattacaaaa
    15781 caccaggcta tgttacaaat aaaacctgaa ctggggtatg gaaaatttat actaaaaagc
    15841 aaggacatta ctgacaaata tgccgatgct tatatgatca gtgtccttga taaattttcc
    15901 atcacttacc tggaggttga aaccgattct ctccattacc agtatggcga taagctaaaa
    15961 gctaccatat cgcttcacaa tgatatcact gagtatgacg tcaatgacgt tgatgctcga
    16021 cttgtaggcc ctaaaggtca agtcatttcg cttaatctaa ccaagttaaa atccaatgta
    16081 tttgaaggaa ctgccacttt ggattccgag ttaaatgatc gcggcgaaaa ctggtactta
    16141 gaaactgatg ttcaaacaga atacgggcaa gaaatcatta gacgatctgg ccatacagcc
    16201 ttttcatact ccattccctc agccagtctt atgaatgtaa aaaaattgtc ctctaagcct
    16261 ttgacttttg ttgtcacagt agatgttgca actgcaagcc gatacgcttt acaaagtgtg
    16321 ttgttccaaa aaaatggaaa gggtgaagcc aggcctattc agacctcgca aagagctcag
    16381 tggcttgagc ccggtaagca tgttttgcaa tttacctttg acaatcacaa tcaattatcg
    16441 gacgataacc tctatttggg ttatttacgc ttgatagact atgggcagtt aaaaactgtg
    16501 tatcaataca accaacctgt caagcttagt cagttagtgg attaatgaat ggcattattt
    16561 tcttctgtca ctttgtttga gggcatagcg ttggctattg cctttcaatt cttattgctt
    16621 ttctgggttt tggtaaaaca agcacagcaa aaaaaactga ccgaacaact tggtgaaaga
    16681 atacaacaaa cactaatctc tcaacttcac cagttacata ttgatttaag ccaatcatat
    16741 caaacgagcc aacaatttat taatgaaaag atagctcaag gacaactggc tacacaacag
    16801 ctcattagtg atgccataca aagacaaatg gccgatgtca gagagcaaat gactcatagc
    16861 ttcaaacatc atgcgagctc actcacttct cacttacaat tactaactga ggaaattcgt
    16921 aatcatttaa acacactaac tcagcaggtt aatcacaaat tgaccgaagg ttttgagaaa
    16981 acttcgtcta cttttattga cgtggtaaag cgattaacca tcattgatga ggcacaaaag
    17041 aaaatcactg aactctccaa tcatgttgtc agccttcagg acgtattagt ggacaaaaaa
    17101 gctcgcggtg cctttggtga agttcaatta tcaacactca tcagcaatat gcttcccagc
    17161 gcaagttatc aaatgcaata taccctcagt aatcaaaaac gggctgactg tgttttgttc
    17221 ttacccgagc ccactggaaa tattgtcata gacgccaaat ttcccctgga aacttatcaa
    17281 cgattaatca acagtgatgc cttatccaca gatcgtaagt cattacaaca acaatttcgc
    17341 caagacattc aaaaacatat taaggacatc gctgaaaaat atatcgtgcc caatgaaaca
    17401 acagatggtg ccatgatgtt tattcctgca gaatctatat ttgctgagat tcatgcaaac
    17461 tatcctgatt taattgccat gtctcaaaag ctaaaagtct ggctggtatc gcctagcaca
    17521 ttaatggctg tactgactac agcaagggca gtacttaaag atgatgcaac tcgcaaacaa
    17581 gtacatatca ttcaaaaaca tcttcaagcc ctggccaatg attttcaacg ttttgaaaag
    17641 agaatggata aattatccaa acacattgac ttggcacatc aggacgttaa tgaagtcaat
    17701 acatcagcca aaaagatcac ttccagattc caaaaaattg aatcagtcga tatcgaattg
    17761 gatgaacacg aagtaactca attagaattg gtcgagtctg aatccatcta aaataaaatc
    17821 tttatggtgc agaataccta atttagcatt atactaagcg gttttcagtt ttaatcatta
    17881 aattccaatg aatacaaaaa cgctacttta ccaacccaca aaaaataaac aaatatgggg
    17941 gcaactctat ggtagcagtc tggcacttgc tttagctgaa tattgccaac aaacacccgg
    18001 gatcaaatta cttattacgc aagacaacct atgcgccagt cagttgcagg atgaactaca
    18061 attttttcta aacccgaacc aaaatcccca ggaattatta ttttttcccg actgggaaac
    18121 cttgccttat gatcaattct ctcctcatca agatattatt tcagaaagac tctacacctt
    18181 aagcagaatt caacaaatta ccaatggaat catcatttct tcagccagca ctttaatgca
    18241 cagactttct ccgccagagt ttttaaatca atacgcttta atgcttaaag aggggcaaaa
    18301 gcttgatctg aactcgttca ggaatcagtt gcaacaagca ggataccatt gtgttaataa
    18361 agtgcttgaa catggtgaat acgcactacg tggatctatt attgacgtgt atccgatggg
    18421 tagtggttta cctttccgta tcgagctatt tgatgatgaa attgaaagtc tcagagaatt
    18481 tgatccggaa acacaacgca caatagaaaa aatcaatcaa atccatttgt tacctgctcg
    18541 tgaatttcca ttaaatgaac aaagccaatt acacttccga cgtgctttca gagagtcttt
    18601 tcctggaaat cccagccatt gcccaatata cgaagcaatt agtaacagtc aatttccatc
    18661 tggcatagaa tattatctgc ccttgttttt tgataaaacc gttacctttt ttgattatct
    18721 gccagccaat gccaaaattt gtttaattga agaaatccag gagaatgctg agcgattctg
    18781 gcgagaatta aatgaacgct atgaacaacg gcgtcatgat gtcagccgtc ctattttaac
    18841 cccagataac tgctttatta atcccaccga gttattaacc aaagctaaca attatgaaca
    18901 agtacgcctg tatcataaac cttccgaaaa aaaaggagcc ttaaatttca atattgccaa
    18961 agctccacaa ctacccattg acaggaaaaa tattgagcca ctccgccaat taagtgagta
    19021 ttgttcagat ccaactcgac gctatcttat tgttgttgaa agcgctgggc gtcgcgaagt
    19081 attactggat ctgcttaaat caagccatat cattccaaga acacaacact cgtggttgga
    19141 cttcattcat gatgaatccc ccatcaatat aactactggc ccattgattt atggctgtga
    19201 attaaccgac aaacaaattg tgatcatcgt tgagtcgcaa ttatttggtg agcaaagcac
    19261 acctcaacgc cgaagcactc aaaaatcagt tgatcccgac ttaatcatac gagatatggc
    19321 cgaacttcga ttgggcgctc ctgttgtaca tcttcaattt ggagtaggtc gctatcaagg
    19381 tttgcaatac atcgactcca atggtacccc cagtgagttc ctcgtccttg cttatgcagg
    19441 ggatgacaaa atctatgtcc cagtcacctc tctgcatatg ataagccggt atactggtgt
    19501 ggacagtgaa cacgcccctt tgcataaact gggttctgat caatggcaaa aggaaaaaaa
    19561 gaaagcggcc gagaagattc atgatgttgc aattgaatta ttggatctct atgctaaaag
    19621 agaagcccag ccagggcacc aatacgattt caatcaatcc gaatatataa aattcgccag
    19681 tggcttcccc tttacagaaa caccagatca acttcaggct attgaacaaa ttattaggga
    19741 catgcaatca ccaagaccga tggatcgcct aatctgtggt gatgtaggat ttggcaaaac
    19801 agaagtcgcc atgcgtgctg catttgtagc agtacaaaat ggaaaacaag tctgtatcct
    19861 tgctccaact accttgcttg caggacaaca ttttgagtca tttcgcgata gatttgcaga
    19921 gtttcctgtt aatattgaat tattatcccg ctttcgcagt aataaagagt ccgaagcagt
    19981 ccttgccgca ttgcaatcag gtaaagtaga tattgtgatt ggaacccata aattattcca
    20041 aaataatatt tctttcaaaa acctgggatt actgattatt gatgaagaac atcgatttgg
    20101 agtgaaacaa aaggaacata ttaaatcgct tcgtactcat gttgatattt tatccatgac
    20161 tgctactcca attcccagaa ctttgaacat ggcaatggcg ggaatcagag atatttcact
    20221 tattgcaaca ccgcccgcca agcgtttggc gattaaaaca ttttggcaag aaaaaaatga
    20281 tctcaccata cgagaagcga tattgcgcga aatattaagg ggagggcaag tattttacct
    20341 gcacaataat gtacaaacta tagaaagagt ttgccaggat ctggaagctt tagtgcccga
    20401 agcaaaaatt caaagcgcac atgggcaaat gcgagaaagg caattagagc gcatcatgtc
    20461 tgatttttat catcaccgat ttaatgtcct ggtttgcaca accatcattg aaacagggat
    20521 cgatattcca acggccaata ccattattat cgatcgtgcc gataaatttg gattagccca
    20581 gctgcatcaa ttacgtggtc gtgttggacg ctctcatcat caagcctacg cttacttatt
    20641 aactcccaat gaaaaactgt taacgccaga tgcagtgaaa cgtctcgaag ccattgtgtc
    20701 tttagaagat ttgggagcag gattcacctt ggcaacacac gacctggaaa ttcgtggagc
    20761 aggtgaactc cttggtgaag aacaaagtgg taatatgcat gccataggat ttaatttatt
    20821 tatggaaatg cttgaccgtg ccgttaacga tttaaaggca ggcaaaactc ctgaattgtc
    20881 agcaccaatg catcaaggac ctgaaattga cttgagaatt agcgcgatta tccctgaaga
    20941 ttacataggt gatatacaca atagactcat catgtataaa agaattgcta atgcaaaaac
    21001 tacccaacaa cttagagaac tacaaataga actgatagac cggtttggtt tactcccaca
    21061 accagtcaaa catctctttt tgattactga attaaagtta aaagctgcac agctcggtat
    21121 tcaaaaaatc agtgccagtg ctcaacaagg taaattggat tttagtgaaa aaccatcgat
    21181 tgaccctgga acactgatta gcttaataca agtacatgcc aaacgctatc aaatggaagg
    21241 accacaacgt ctgcgtttta ctttagacag cacgtcagca gaggaacgaa tatttgaaat
    21301 cagttcttta ttaaataaac tgtctcctca ttcccaaacg attgactgag atttctgcag
    21361 tgccttttgg attaactcag cataatcttt ttcaactacc cttctcttta ctttcagtgt
    21421 aggtgttaat ttatcatttt cagttgtcca ggcatctttc agcacaagga tgtgactgac
    21481 tttttcgtat ttaaccaatt tggaattgac ttgctttaag gtttcttgca atgcgtgagt
    21541 gatctcttct tttggcatac ccaaacgcac tccttcattc aaggtaacta acaaaacgtt
    21601 actaggtaac tctctaccta ccaggcacag ttgttctagc atagggttca ctgaaaacag
    21661 tttttctatc ggtgaagggg caatgaactc tcctgtctga tttttaaagt tctcagaaag
    21721 tcttcctaat attttaactc tgtcttcatt atcaacatca acaacatctc ctgttcgcaa
    21781 ccaaccctct tctgtaaagg catttttagt cgcttgttca tttttataat aacctgacat
    21841 tagacaaggg cacttcatta gcaattcatt ctcttcgcct acttttatct caacctccag
    21901 cctcggagta ccaacgtaac cacgttttct ctcatttaac atcgataagg tagcataagc
    21961 caaattctca gattgaccat atccttcctg aatataaatt cccaattttt caaaaaaatc
    22021 aataatggat attggcagat gagatgcccc gctaaaacaa ttagtacact cgcttaatcc
    22081 taaactgtga atgatctttc tttttatcag actggatata agaggaattt ttaataaaaa
    22141 attcaatttg gctggtggca gtttttgttc aattttctgc ttaaatactc cccaaatgcg
    22201 tggaactgca gtgaaaaagg taggactcac ttcccttaag ttctctgcaa acttctccag
    22261 actttcaaca aaagataccg atgcattaat agcaacactg cctaattgga tggcagagcg
    22321 ttcatagaca tgagccaggg gaagataaga tattaattta taatgttcca actctctgat
    22381 tctgagcaag tccttgggga aaactgtcaa gtaattggct atggaccgat tggtatacat
    22441 agcccctttg ggtgcccccg aagtcccgga tgaataaata atggtataca acgcatctgg
    22501 ctctggctcc accaggttaa tcatagggtc acttttcaaa acatcagacc attcgtaatc
    22561 aacttgcaaa tctttatggt aatcgaaacc aattgtagga tagctcttag ggataaattg
    22621 tctgacccgc tgatgatcat ctaattttcc aacaaaaacc agtttcacat ccccatgctc
    22681 taaaacataa tgcgcacttt cttcattttg attagcaaat agcggaacat tgaccattcc
    22741 agcaatatga attccaaaat cagcgataaa ccattccgcg caattcttgg agataatgga
    22801 aacatgatcg ccttttttta accccagatg gtgtaaaaaa ccagctactt ttctggcctg
    22861 cagcatcaca gacgaccagg tgtattcatg ccaaactcca ttttttggct gtcttaaata
    22921 cacacggtct gacgaatttt tttcattttt caacagtaag tcaaataatg aattggaatt
    22981 ggattctttt tgcatggctc ttccttaagc ttgtttaatt actattctag accaactagt
    23041 tgtaaaatat tatttttttc tcgcaaaaca caattttcac cttcatgaat cgcctctttt
    23101 gctttatgaa aatcataaag catgatgttt cctaattgag gaaccaaagt caaatcagca
    23161 ggatcgcctg ccgctcttgc tcgggttatc cggtcctgca ttattttgat gcttctggtc
    23221 aaaacatcat aatatcctgg cccatcactg attcccatta ttctttttat cattttatca
    23281 aattggcctg agagaactga aaaaaacccc gggtgttctg tcgtgctcgg aggtactctt
    23341 agcaagtcat gatttaaatt gacagcgatt acaacatctg cccccatagc tcgacacaat
    23401 gtaataggaa ctggattaac caagccacca tcgactaacc agcaatcctt ataacgctgc
    23461 ggggtaaata accctggtaa agacatggat gaacgaatag caagctcaag agacccttta
    23521 cttagccata tctcacgtcc agtactcaaa tcagtagcaa ctgaactaaa aggtagcgag
    23581 agctcctcaa tatttttttc aagaccaaaa tccttgaaaa aggtcatcaa cttactacca
    23641 gagagaaccc cgccacctaa aaaattcaca tcaatgagtt tagccatttc ccttttatta
    23701 agctttaaca cccattgttc aaacagatcc agggtgccac aagcataaat ggcgccaacc
    23761 aatgcaccta tggaacatcc agcgacacaa tcaatcctta cccccaatcg tttgagtgat
    23821 tgaattaccc cgatatgagc ccagcctcgc gctgatccac tacctaaagc caaaccgatt
    23881 ttagggtatt gatttgtcaa gaataactcc tggaaatagt tgggtacaca ctcgttaaat
    23941 caatcaattt catgtttttt aacaccggat gtcactaaat ccaaaaacca aatgtagcgc
    24001 aatgctaaac tgacattttc ttacttggtt ccgccaaccg tcaaggcatc aattttcaaa
    24061 gtgggttgtc ccacgccaac gggaacagat tgtccttctt ttccacatac accaatccct
    24121 ctatccaatg ccaaatcatt accaatcata cttatttttt tcatcacatc agggccattg
    24181 ccaattaaag tagctccttt cacaggtttt gtaatcttgc cattctctat taaataagcc
    24241 tcactcgcag aaaatacaaa ctggccagaa gtaatatcaa cttgcccacc tccaaaattc
    24301 accgcataca atcctttttt tacagtacga ataatttcct gagggtcgta ctgccctgcc
    24361 agcatataag tattggtcat tctcggcatt gggacatggg cataggattc acgacgacaa
    24421 ttacctgttg attgcattcc cattagtttg gcattcaact tgtcctgcat gtaattcact
    24481 aaaattccat tatcaattaa ggtcgtgcat tgcgatggcg tcccttcatc gtctatagtc
    24541 agagaaccac gtcgattctc taaagtccca tcgtccacaa ctgtaacgcc tgcagcagca
    24601 acctgctgac ccagtctccc tgaaaaagcg gataatccct tgcgattaaa atcaccctcc
    24661 aaaccatgtc caacggcttc atgaagaagt acccctggcc atcctggacc caaaacaact
    24721 ggcatggttc cagcaggagc tggttctgcc ttcaaattgg ttaatgcttc ccgtaccgct
    24781 tcacgcgcat aactcaaggc gttttctttc tcggtaaaat aggaataagc tactcgtcca
    24841 ccaccgcccg atctcgccga ttctcttcta ccatgactgt cttcgacaat aacactgaca
    24901 ttaatgctaa ccaatggcct cacatcagcc atcatctgcc catccatatt ggcaaccatt
    24961 actacttcat agcaaccagt taaagtggca ttaacctgta tcactcgagg atcaagacta
    25021 cgggcttctt tgtcaatggc ttccaataaa gcgattttct cctgcttgct cattccttcc
    25081 aaaggattta aactctcata tcgatttaca gaagccttta ataccttgac ggccggtact
    25141 gtttttgaac cagaaagggc tatggagcgc gccgcatcgg cagcacgaag catggaaggc
    25201 agtaatatat catcacaata ggcaaagccg gttttatcac cgcttacagc cctaatccct
    25261 acccccttat ccagagaaaa attaccgctt ttaacctctg aatcttccaa ataccaggat
    25321 tcataactac aactttggaa ataaagatca gcatcatcga cgttccgatt aaacatggtc
    25381 ttgaataatt tttctatgcc gacttcatcc agggaagctg gtgtcaataa aatatttttt
    25441 gctatcgcaa gagattttgt catttatata ccttttacat taaaacatga tgttcaacac
    25501 acggaaattg tcttctcaac tgtgacaatc tctctaaatc aatatcagcc agaattactc
    25561 cctgcccttc ttctttttga gccaacactt taccccatgg ttccaccatc atactatgcc
    25621 cataagtatg acgcccgttt tcatgagtcc cgccctgatt aggagctaat acataacata
    25681 aattttcgat tgctcttgct ctgagtaaga cctcccaatg cgcagctcca gttaccgcag
    25741 taaaagcaga cggaactgaa aataattgtg cgcctctttg ggttaaatat tggtacaatt
    25801 ccggaaaacg caagtcataa cagatggtta acccaatttt ccccactggc gtatccacaa
    25861 gcgctatatc ttttcctgct tctatagtta acgattcctg gtgtttttcc tgctcggaaa
    25921 ctatgacatc aaataaatga attttatcat agcgagcaac atttaagcct ttatcatcat
    25981 aaactataca acttgctctt acctttgaac ccatgctttt caacgggatt gttcctgcta
    26041 ttatccatac ccgcaaatct ctcgctaact cacttattct ttgttgtata ggtccttgac
    26101 cataatattc agcaatgtgc aacttctctc gttcattcat tcccataaaa gcaaaattct
    26161 caggtaacac aaccaaactg gcttcctgtt ccctggcttg aattagagat tgttcagtga
    26221 tttgcaaatt atctgctatt ttggcagatg ataccatttg cacaagcgct actcgattca
    26281 tccttcttcc taaatgacat tcatattttt catcttacta tatcgttcca tataagccca
    26341 aaacaaaatt gacacaaaaa ccttttcgcc ttaataataa agtgtctatt ggcctatatg
    26401 taaattttat tacaaaattt tcaagtgata cgacttgaaa aataggaaat ttgaccatag
    26461 atatgctaca ctgataataa ataactggag ttctgagcaa tgaaccgaaa tgatatcacc
    26521 atacttagtc aacaaagaga atctgtgctc gcaaccaata aggtcctgcg aaatacgtat
    26581 ttactgctaa gtttaacctt catatttagc gcattaactg cctacgtagc ctttattagt
    26641 ggagctcgcc caatgaatcc actattaatg atagttggcg tttatggttt gatgtttcta
    26701 actcaagctt taagaaatag cgtttggggg ttagtaagcg tctttgcttt tacagggttt
    26761 ttaggatata ccattggccc attactcaac tattacatca ccggtttttc aaatggccca
    26821 caaatagtcg ctactgcttt aggcggtact ggaatgatct tttttgctct atcaggatat
    26881 gccctgacaa cgaaaaaaga ttttagctac ctgggtggat ttctttttgt tggcgttatg
    26941 gttgccttat tggcgatgat agcggggata tttatacaaa taccagcttt gcaattagtg
    27001 atttcagctg cttttgtatt aatttcatct ggattgattc tattacaaac cagcgccata
    27061 attcatgaag gagaaaccaa ttacataatg gccactattg gtttgtttgt ttccatttat
    27121 aacttatttg ttagcctgct gaacctgtta agcgcattct caggtcgcga ctaatttaat
    27181 atcgtctcct ctacctgagt cccggctgta tgccgggatt tttttatgga tcaattcaat
    27241 ctgtgaaggg aaatattctc tgaactttct tccagcctgt ctgactgaag acaaatcaaa
    27301 tggcattttc tgttataaaa ataaagcaat tttatgggtg caattttgag tcagaatacc
    27361 agcggtatac acgccctttc tcttttggtc tggattttgt ctgttattgt ggaggatatc
    27421 tacgattcaa gcaaccgtct cttcactgta aatcaatgag caataatcgc catgtctttt
    27481 aatttgtaaa atacccaaaa aacacaacaa accaggtctt ccatcaactg ccattggctg
    27541 gtggctgcaa tcggaaattc tatccgtgac taaaaatatt cctggtgatg caaggcattg
    27601 ccatataatg ttatcgtgca ggttaaattg catcaaataa caatcaaacc tgcctctcat
    27661 atgaattatg attaatgcca ttcattcaca cacccacgta aacatgcttt gaggtcatcc
    27721 atattgcttg tctcaccggt ctgattagca atacctttac cgcacccaat cagacattgt
    27781 gcaagatctt ttgctatacc gcgatttgaa acacaattgt ccaagcactt tttatcgcca
    27841 ttacatggct tcatacattg cgacatagca tcgacattta ctgaagcttg cgctgctgtg
    27901 aacatgaaca aagaaccagc taatatgaac aataaatatt ttttcattat aactccctat
    27961 tgataatgaa ttagtaacct ttgtaagtgc acagcaattt aaaactattg ataagtgcga
    28021 atgacttaca tccattaaag tatataataa atttttaaat ttatttacga ggcaacagga
    28081 ggcacatttg tggttggggg ttagtgctcg caaacagatt tgttggacct gctcacaatg
    28141 gactcatttt cagtaaattg cgttagaatc ttgttggaaa ccgggttgcc caaatcatca
    28201 cctttattct tatatactga gcaatagtac atcaattttt ttgaggacac attttggcaa
    28261 actcaaaatt atctaaacca caaatcatat tctttcctgg ccattcgtta aatttgcatg
    28321 tctttattat aattacgcag aactcccgaa tgaccacaat aatattcata cttctatagg
    28381 ctgatcctaa aagtaattaa ctgaaacaac cgctgctttt caatttttga gcagttatct
    28441 gaataataat tacattgcaa aatccagagg aattatcaat cgatctgaaa ttaactttgt
    28501 ttccaactag ccttggcttt aaaaagttct ggcaaatctt ccggcctttt cttcacttcc
    28561 tggctcagag taaaaagctc acgatccttt tgataataga ctcccaaagg cactgggtaa
    28621 tcagggaacg tcaatcgagc taaacgcatg gcgctaatta agcttttcgc atcatgctta
    28681 taaagcgctt tttcattggc tgaaatttgc acaaattgct catcatccaa ctgcagcgct
    28741 ttttcttttt gagcgccaaa tacgagtggc tgaccgtctt ccaatattat tgtgttttca
    28801 gcccggttat ttttaactgc aaaatcatca aaagcaccat gattaaaaat attgcaatct
    28861 tgatatattt cgacaaaaga gcatccctta tgttcataag cttttttaag cacagaagcc
    28921 aaatggacag gatctttatc aactgctctg gctacaaaac ttgctccgga agccaaagcg
    28981 atcatgattg gattaattgg ctcactagtc actcctttgg gggatgtttt agttacttgc
    29041 cctttctgag acgttggaga aaattgcccc ttggttaatc cgtaaacctg attattaaac
    29101 agaagtatat tgacattgac attgcgcctt aaaatatgca gtaaatgatt gccgccgata
    29161 cttagcgcat ccccatctcc ggtgataacc cagacacata aatcttctcg cattgccttt
    29221 aagccagttg ctactgctgt agccctgcca tggatagtat gaaatccata agtgttcata
    29281 taataaggaa gccgtccggc acaccctatg cctgagacaa agacgtgttg ctcaggaggc
    29341 aaacctaatt caggaagtac tctttgcaaa gcagccaaaa tggcataatc accacatcca
    29401 ggacaccagc gaacttctgt ttcattcgca aaatcttcac gcttataatt gctgttcata
    29461 gttggcttcc gctttaatgg cacttaccaa atgattaaca ctgaaaggtt gaccattgca
    29521 ttggctaatc gattgcgcat ccacaagata aactgctctg atgagttgac ataattggcc
    29581 agaatttaat tcagcaacca ataccttgtc atattttttc aataaagaac ccaaatcatc
    29641 aggtaatggg ttaagatggc gcagatgcag ataggcaacc ggtaatccct gttcaagaca
    29701 ttgatgtaca gcagacttta aactaccaaa agtactaccc caacctataa caagcgtggg
    29761 agccgtcata tcaccctcaa gaaccaaagc aggataatct cttgcaatac cctttatttt
    29821 ctcggcacgt gtaatcacca ttttttgatg attctcagca tcataactaa ctcgtccttc
    29881 atcgccttgc ttttccaagc ctccaatttg atgaataaac ccagaagttc caggaacatt
    29941 ccagcttcta cttaaaaatt catctcgatg atagggtttg gtaaaacgat taaactccgg
    30001 ttttggtata ttgagagaat ccaaagcagg aatttcccag ggctctgccg cgttcgctaa
    30061 ataagcatcg agtaatacaa taactggagt catgtacttg atggctatcc tgaaggcttc
    30121 aataattgta ttaaaacaat cagcagggga ttgagctgca atcacaggta aaggtgcctc
    30181 gccatggcga ccatacaaag cttgttttaa atcactctgg ctggtttttg tgggcaaacc
    30241 tgttgaagcc cccgctcttt gcacatcaac tagcaccaag ggcagctccg tcacaacagc
    30301 caagcccagg ctttcacatt ttaaatcaag cccaggccct gaagtacagg tcagagccaa
    30361 acgaccacca taagcagccc ctatacaaga acaaatagcg gcaatttcat cctcagcttg
    30421 tattaactgt acgccgtaat cagataagcg aacacattca tgcaaaatag cagaagccgg
    30481 tgtaattggg taacctgaca ccaacagagg aacttgcgtc tgagtagcta atgtagccag
    30541 agctaaccct actgcctcaa ctccagtgat ttgcctgtac tcacctgaat gtctgttgac
    30601 ttcccccagc atgtaatccc gacgggaaag ctccagagtc atagcataat tatatcctgc
    30661 caacaaagcc aattcattgg cttttgctat ggcctgattt gttttaaact ttttagcgat
    30721 aaaagcctgg caagtttctg taggcaaatc aaacaaccac aaaaccaatc ccaaaatata
    30781 aaaattcttg gttttgacag cctgtggttt tgtaaggtta atggattcaa ctgcctgaat
    30841 agtttgtgta attaatggga aagcaacaat gtgataatgt tctcgacaat tatccaggaa
    30901 actcttatcg atacctgctt tttgccaatc tctttcttga aaactgtctt cattaatgat
    30961 cagcaaacca ccttgattca aatgttgaag tgagtttttt aatgcagctg gatttaaagc
    31021 cactaaaaca tccaatgact ctccggcagt aaaaatagct ctttccgcca ttgccaactg
    31081 aaatcctgag acacccgcaa ctgttccagc aggagcccgg atttctgccg ggaaatcagg
    31141 catagtacgc acatcacgtc ctgtcaatgc cgcgctaatg gttaattgtt cacctaccaa
    31201 ttgtactccg tcaccagaat cgccagtgat gcgtacaaca atgacatcag tcgttgacat
    31261 aaagtctcgc ttcctgcatt aattggcgca tgtgtcttac tgcctctttt aatccacaga
    31321 ataatgctct ggcaataatg gcatgtccta tgttaagttc atttaactcc ctaatcgctg
    31381 caatcggttt aacattatga tagtgcaacc catgaccggc attcactacc aagttcaaac
    31441 tggcagcaaa ttcagctgcc tctttaattc gttgcaattc ataatgctgc ttttcctctg
    31501 aggtagcatc agcataacac cccgtgtgca attcaataac tggcgcccca acatcaaccg
    31561 ccgctcttat ttgctcttta tccgggtcaa taaataaaga aacctcactt cccattaatt
    31621 gtaagcgacg tactgccttt tccaccacat ttcggtgagt caaaatatcc aatccacctt
    31681 ctgtagttag ttcttcccgc ttttcaggca ctaagcaagc atgctccggc gatatttcct
    31741 cggcaaaatc aagcatggct tcagtaaccg ccagctcaag attcatacgt gtttgcagaa
    31801 cctgtttaat caatctgaca tcacgcgcct gaatatgtcg taagtcctct ctcatatgca
    31861 aagtaattcc atctgcccca gcttcttcag catccattgc tgcctggaca ggatcaggat
    31921 aacgagttcc tcgtgcttgt cttatggttg ctatatgatc aatattaaca cctaacaata
    31981 actctttatt cattttgcac caaaaaatat aaaatgcaga gtataatgca gttcttgaca
    32041 aactgctatt cacgttacct tgtttcaatc agtttaaacg gcactaccta aagcatttaa
    32101 ccatacttta ctttattcac tac
//
LOCUS       DATNSN010000020        31132 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.020, whole genome shotgun sequence.
ACCESSION   DATNSN010000020 DATNSN010000000
VERSION     DATNSN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 31132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31132
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.020"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            315..512
                     /locus_tag="VAH76_000739"
     CDS             315..512
                     /locus_tag="VAH76_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO1385387.1"
                     /translation="MKSAEIIKIIERDGWVQCHQKGSHCQFKHPTKKGRVTVPHPKKD
                     LPIKTVASIFKQAGINKEDIK"
     gene            509..913
                     /locus_tag="VAH76_000740"
     CDS             509..913
                     /locus_tag="VAH76_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO1385388.1"
                     /translation="MRYPVVIHKDEHSDFGVIVPDIPGCYSSGSTYDEALTNVIEAIE
                     CHLEGLLIDNEPLPVGTTIDRWINDEEFQGGVWAFVDIDLSQISGKAKRINITMPERI
                     LSLIDLYAKNHAIKNRSSFLADAALSYMESHK"
     gene            complement(1485..1808)
                     /locus_tag="VAH76_000741"
     CDS             complement(1485..1808)
                     /locus_tag="VAH76_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385389.1"
                     /translation="MSRLTISLPDNLHQHISALAIKNNDSMSNMINQLIQVGLHHWFK
                     NDGNFKANPAVEKYCHQLIIQMNALIKNMSIELLKLDREDFEKLQQAAAIKYSELKNS
                     AHENL"
     gene            complement(1825..4773)
                     /locus_tag="VAH76_000742"
     CDS             complement(1825..4773)
                     /locus_tag="VAH76_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HEO1385390.1"
                     /translation="MLSVEPLKSAQGAADYYSKAFNYYSGDATAMQWLGQGSQKLKLS
                     GVVQKEQMLALLEGRLPDGQVLQNLEGEHRPGFDMTFSAPKSVSVLVGLGLAPELVRF
                     HDEAVQLTIRQIEKEFAETRVSRRGEISFEKTNNLLVAAFRQPSSRANDPALHTHCVT
                     MNITFHEGKARSLSSDPSREHGVIEQIQNNAHYCGLIYRQHLANKLKETGFHLRLTGK
                     GLFEIDGVPDEVLHEFSTRRMDILGYMDEKGWNGAKSASTATVLTRKNKEEHDINLLT
                     EDWKQRAKDIGFDAQAFMQHRDKSQSISWLSGVKDRLLALVNKYKKDSSPSEIEAAHA
                     CVVVAIETLSQRTSIFSERMLTQEAMKHSLIYPKAVSRQSIIESIQQEIKSQTLYEAR
                     CFDRGERLLTTPWLLTLETETIARIDRNKGAVPAIAHLNAVTAFQKMRSSLLPYPMTN
                     SQKESMKVLLTSKDRYLAIQGYAGVAKTSMLSEARIFIEAQGHKLRGITVASSAAHEL
                     QEKAGIKADVFPLVHQELKEAQTASLTKTLFIIDEASMLSSHQGHELMKQIERVGARL
                     VLVGDRAQLPSVNAGRIFGLTQEYGIETAVMDEVVRQKNEILRQAVIATTKGFVQEAL
                     DNLDVKTLATHKERIAWIANHWLSLSASARDETLLFAPTHANREEITTFIRSGLKQEG
                     TLKGEQFSQIILKTKMMEPIQSRFVAYYQKGDMVRFNQSFKRNKIHAGSYYTVGEISK
                     KHRQDNVLPLIDESGNLIKFNLNNLPNYKTHTAPFERTIEVYETKSLELLVGDKVMWT
                     RNFKANDIRNGQCATLHEIKNNSFMFITKEGRLLTLEKSHPALKHLDYSYVLTNYKVQ
                     GKDAPYGIGLMESFHRFGSTLNNFYVQISRAIHGMTLVTDNKEELVHAIRRNSNEKPA
                     SMDITSSAQLIHHERRFGAQNQLSIQSVIDKKKSLEIQEISKSMNQDDLIIEKKGPHL
                     TKQLSKELEL"
     gene            complement(4788..6623)
                     /gene="traD"
                     /locus_tag="VAH76_000743"
     CDS             complement(4788..6623)
                     /gene="traD"
                     /locus_tag="VAH76_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HEO1385391.1"
                     /translation="MREEPNFKHYTRGGQISFHNLRMWDQITKTLVWVCLFLWVVLTG
                     LITWLVISPEKFHQAIAYYYASFLNLVGQKHTFTLPFHGKNYQQNVETILHYPYYKEN
                     ADHMVDLLGKSSLAAFVFSFVLGLCFAFYFIHRGKAQRANQFIRGSRIDTKENVIQQI
                     LDKKENSDITIDGFPLKANSEVQHLLVHGTVGTGKSQLIMKIMDALRKRGDRVIVYDK
                     GCAFIPHYFDSNQDVILNPFDARCPNWDMWLEAPRDSDFENMAESLIPMHGDSEPFWV
                     NAARTVFSSVAAKMRKDKDRSVEKLLKLLLTGEFSHLEDYLKGTAAATLVSGKIEKTA
                     ISIRSVITTYLKSLSTLSGLDSEGASPFSIRDYILNEQHSGWLFISSNGEQHKSLKPL
                     MSMWLAMASLTLLSLTPNPDRRIWFICDELPSLHKLPLLGETIAEVRKFGGCFLLGMQ
                     SFSQLTKVYGQSGGAKELFDLLNTRFFFRSPSADMARLVASELGEEEIEESRENYSYG
                     ANSIRDGISLGSQRVTRPIVSYPQILELEDLRCFVRLPGSYPITQLTLELENRPKYLK
                     GFVERQFIIDEMLQKLVKETESTEDEAIAKRKCHNKKSVEIEFEV"
     gene            complement(6715..7302)
                     /locus_tag="VAH76_000744"
     CDS             complement(6715..7302)
                     /locus_tag="VAH76_000744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385392.1"
                     /translation="MDPKQLKQAIAEDMKTIKMLNPEIIPARFYYGGLLKGVFNGVWR
                     MSIILFLTLCYVMSDDKEPISFSSLFIDSGITALFLSIVAMLILLTPISFFVQFQFHL
                     EKKLKTSALIRKKCNHISMVFFGMFAFLCILFGSYASGQQIFFMLVVSFFLSLGATHI
                     AVHMELSRIGFSSLFTLCNEFFSKGKTVSIEEAQK"
     gene            complement(7304..10111)
                     /gene="traG"
                     /locus_tag="VAH76_000745"
     CDS             complement(7304..10111)
                     /gene="traG"
                     /locus_tag="VAH76_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HEO1385393.1"
                     /translation="MITIHVLAGGELFQHVLNAISAFMKQDSFLGLLRITALIGIVMA
                     TIGFIKTRDPMAFARWFLGYVLFVNLVILPKTSVMIDDISSQTPKLVDNVPVVFALSS
                     SLVTTIGYGLAQSYDALLTMPNDLQYTKTGALFGSRLVSASTSFRIKDPVLKEEMNEY
                     FRICVVGDIRLNRKYSVSDLAHSTDIWNLITVKASPLRMLSVNGKLVSCLEASKPDGQ
                     YSLRKKLDAEIKKAYTFFGVNLFGKPKNTTYEALFATHLKSAFEYYQGLTDSSSNIFL
                     QSMMINAIGDGVAHYQAFTDATAGIVNQQFTKSQVQHRWSWEVLGQKALWILPITHTC
                     LTLILFGVFPLIIALTTLPGGIRILYGYMQFFMSLQFWPVLFAILNAGMTIYGASSSG
                     EYGQFTMVNLDKIDELHADISGACGYLMMLIPFLSHGLVSNLGGAFSNLATSMMSHMQ
                     GSSMSVAAEAASASFGLGQTSFYNTTANNFSANKHDSNWTHLHGMRTSQLGSGVLKTI
                     TGSGDRVFDVSPGMTRSAVSINALEGLSASLNQAYEQSTQAARNESAHYQNSLSNFAH
                     RALQLSQLAGHDMRLGDSISESETGQYSKALSTISHIAEDVARRLGVSKEDALTHLTS
                     GGLSAQVGFKSQGSIWGKLAQWGTGFHASADAHLKFDRNSTSQDKYHTGMDGSLSSRE
                     SRDFNDAISYVKQFSQNHHFDDSHSQAASLSNQLGADLREAETASRNVDASLAKATRI
                     QNAKNFVESHSSQITTDLNQAFQAYVAARLNESERDELYSHPGDLRALNKLQALGHDF
                     IASKRDELIAQFGNTEHKSQIESFYQKEKSGFIAKENQLGSHYQKNSEEFLHQAQSMK
                     VGINDKELGVFHQEVHQQKSTLPQNLKQGEQLIKSGSRDLMNEKDLRLQEGMMNAQKN
                     VVVPENMHLPFEDKKLHKKGE"
     gene            complement(10123..11487)
                     /locus_tag="VAH76_000746"
     CDS             complement(10123..11487)
                     /locus_tag="VAH76_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HEO1385394.1"
                     /translation="MKKAVITLFLWAFFPVVQANVSQDLDNFFNGMGYASNVTSPDAF
                     ESQAAGFFGGGSLYARNQVRQYQLVQLDLPSYRAGCGGIDLYTGSMSFLSEQKLVDLG
                     KSVMTNAGAYAVDVMLASTVPELKQVRDYLQQLEQMANQASINSCQLSQNMVGGIWPK
                     TAESQQKICKDQAAMGKEGLFSDYVKARMVCAGTGFDNVMSKASQDPERKKQVVLNKN
                     LVWSLLQAKSFLNSDRELAEMVMSLTGTLIIDKEGKVTNVPSLAGNADLVNALIGTGN
                     GPRSAKIWRCKDAGSNSQCMQVSLQDITIQETSTLTYKVREIIRTINTKLINDEKPGN
                     RELNFLSMTSLPVMKFLSVLNSMHYGSSAVDIEEYSMLIAQDLLTNYLIELLREVSVA
                     TAGSELNTDLVKEIQKRINEATTHVESLDPKVGRKLQEKLNLIERMAQIEKQVSSQMN
                     STAG"
     gene            complement(11487..11993)
                     /gene="trbB"
                     /locus_tag="VAH76_000747"
     CDS             complement(11487..11993)
                     /gene="trbB"
                     /locus_tag="VAH76_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HEO1385395.1"
                     /translation="MFARVFLIVMLAFIGLGAKAEGSQWLTNLIAEQEGLVTHQREEP
                     QNTKRKGFFSTHGLILFYGSQCPHCKRFAPILKGWANRNKAEVLPLSLDNQPLPEFPK
                     FLPATTEWINAAFGGNSINYPALFVVNPKTKTLYPVGFGSMTNAELNDRMAVLISKIK
                     AYEIKGRL"
     gene            complement(11993..12733)
                     /gene="traF"
                     /locus_tag="VAH76_000748"
     CDS             complement(11993..12733)
                     /gene="traF"
                     /locus_tag="VAH76_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HEO1385396.1"
                     /translation="MINRMLIVFLLLSGVAFADKPVGFLWYTKERETKRVEKKTSGIP
                     FKSLSYTERDAVLRFYTMEALHKARYTKKMEDMRVFLSLQDYWLKESSRFKSLFQKTM
                     LAHPEYDYAVTHPTSNLGAKITDEVRESQTIEVINRLSKSHGLLFFYRGKSPYDLKQI
                     PIIVDFCKRFRIQLIPVSVDGATSPELLNSRMDQGQANRLGVRYFPALLLVNPASQQV
                     SPVAYGLTTQDVLMERIKQVVNQFKGEE"
     gene            complement(12726..14417)
                     /gene="traN"
                     /locus_tag="VAH76_000749"
     CDS             complement(12726..14417)
                     /gene="traN"
                     /locus_tag="VAH76_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HEO1385397.1"
                     /translation="MSKVGLIFFCILSFSSFSQNQVDALHARDEALQNLKGFNPATAL
                     KGYTINPQETSLQPQEGSNPFASLGLNALKNNSTAHEVYNKALNRSRVRANPNTPEMQ
                     YAETILGDPDGVLEGTCYKEASHCKIESTVKSCEESLQYSMVSCKEMTLSVTVKSLSM
                     SFSRTVVPQRYKSVTTFDLTACSPADRQCSPANTVILTQPCEEISVHVTHHGQPLRVT
                     KQPTCTDTTVTVQLSGNGRLSVPLQVTVTEYVSDDQWQTQVCEPIQSENLNGYCFLEE
                     SQACLEPNQVKVIGGVAIKRPCWGRALQYQCGSVQSTTCTEYINQGCSQTASTCVKSK
                     ANRCERYSQTFQCMQQFCMPEKTACPGKMYCIDGQCDTSRDETSDDMAEGISRLGLLA
                     GVAGDVTANQVRSGTPAIFNGSAQECKKYPLGFRDCCTDSGWGDWVKHCPEALQVLQR
                     AKHENRVVYLGSYKNHKLGARHYTFCIFPTKLASIVQIQGRGGQLGIPYGTAQYPDCR
                     GLTPEELERINFASLDLSPIQEELVARMVLPKNDSLKQTNQSHIERLKQQGRAHD"
     gene            complement(14410..14832)
                     /gene="trbC"
                     /locus_tag="VAH76_000750"
     CDS             complement(14410..14832)
                     /gene="trbC"
                     /locus_tag="VAH76_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HEO1385398.1"
                     /translation="MLRKGILVLIGSVIMTSSFAAQISVLVSFSIPETLLQETLKESA
                     QLNIPAYLNGLYRDSMSETALKVMALSKHIPNLNLAIDPTLFERFGIQQVPAVIVDDG
                     QTFDVIYGHLSIQEGLARMAGRGDVDFTPRDIRRVIGE"
     gene            complement(14846..15829)
                     /gene="traU"
                     /locus_tag="VAH76_000751"
     CDS             complement(14846..15829)
                     /gene="traU"
                     /locus_tag="VAH76_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HEO1385399.1"
                     /translation="MYFRVLLLTVLYLVSYQSSAKLCTGHFVNPITDVCWRCLFPLSI
                     GNAKVVNSSLPDTENASSPIGVCPASTGVRLGLNIGFWEPMALTDVTDTPYCLVNLGG
                     TQLNLGLKQGRGGRHVVGNGQQRAFFHVHWYKYPLINWLNLITSSGCLQKGDFDIAYL
                     TELDPTWSDSEMSFVLSPESVLFGNPVAASACAADALSSTIASKPLDSLFWCAGSQGT
                     HYPMTGHVNASVSPVQTAWLLTERMNYKMHREFLVSDSSPKSDAICKEHYYTVTPKSR
                     YRYELVNQVADGKRCYPGGSSTLAWEAGKIKPHTPDQYGFLVWRKRNCTFL"
     gene            complement(15819..16454)
                     /gene="traW"
                     /locus_tag="VAH76_000752"
     CDS             complement(15819..16454)
                     /gene="traW"
                     /locus_tag="VAH76_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HEO1385400.1"
                     /translation="MNIIFYLFLLMISMGQVHAKSFGVVGEVFPIAEKSFLRLIEERL
                     ASLKASGELKSLNQRWIQTAAEHANRPPALNIVRTHHSIKHEYVPEITLSQDITNEKG
                     QILYSKGTHVNALQHMPSYTPCWLFFNADDDAQLNWADHQKTQCANPKLILTGGAVNK
                     AEKRLDSVIYFDQAGKITSKLHIGHVPAKVIRHQNSLVIHEQAIKENGDVL"
     gene            complement(16451..16786)
                     /gene="trbI"
                     /locus_tag="VAH76_000753"
     CDS             complement(16451..16786)
                     /gene="trbI"
                     /locus_tag="VAH76_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HEO1385401.1"
                     /translation="MRLTPLFLGCALAVFGVVLGFYGVSPQKSIVIFDKKEVLSQFIR
                     QLAEAKATESQVEQSTRRFNALLNKVLVDTAQQKKVIILRASDVLAGGVDITDEMRIK
                     MSHAMRNKL"
     gene            complement(16777..19374)
                     /gene="traC"
                     /locus_tag="VAH76_000754"
     CDS             complement(16777..19374)
                     /gene="traC"
                     /locus_tag="VAH76_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HEO1385402.1"
                     /translation="MGVNFSLWRDKARDLLHRTSHLLGETSGVKPDSLCSYDKAYESC
                     TLDLPSIKSLLPYETVNEAGFFVNRNSMGFGLLLMPMSGADESLMNSLSQLFKNKLTV
                     GTDCTVLLYKHPWIGEQLAQNYEPILKQGGIYAELARETLKYHLNAIKKGYKNGRNVP
                     AGLSDYCGYLFVSRPMQSDVEGQLQLLLADFESELKVAGFGIRRCGEDEFKVLMRALI
                     SPDFNELSWPIINESDGFIAEAIPCPSTVIEIGDEAIEVSITDSQAQTVRTRLVNCEV
                     SHYPAKPFALWQTPDLFANLLDTEQGIQCPFLISFTIRGMNQEKMKAKARARAKSLNA
                     NNNPVQSFINPSIREEAAEWQFVHDHASKGELHLLPTFYNVILYTTSDHEREHVAKAI
                     ASFRQMGFTLTQSRCKQWLRFLGSLPFICTSGLFSSLELLGMTKKLSHANVANLLPVI
                     ADFKGARQGLIVPTYRHQLFYLNTFDDRVLPITNFNRLTVASTGAGKSFFEQAQILDG
                     LSRGQQIFVIDLGGSYKHLCEMVGGSYIDASTLTLNPFTLFDFDGVTEIKGEQVNDYI
                     QIRDLLAIMASPSEALGEVQKAWLLDAVTECWKARGRHAKMDDVLTSLQRMLEKPQSQ
                     GDQRLKDLLILMSKYGSQGIYGHLFNSDTPLLNGSNLVVLEMGELESNPELLTIVMFV
                     MIVIIQGQFYHSDRRREKRCIIDEAWRFLARGSNPVCASFIEQGFRTARKHMGGFAVI
                     TQNLLDTMNTIQGRAIAASSDTKIIMRQGSFKQYLEEHPKHFSPLEARVIESFGEAKT
                     QGFSNLMIQFGNVTTFHRYFCDPFSRVLFSTSGDEVSLIESLTKQGVSLSEAIKRVAN
                     KYYGEELCD"
     gene            complement(19365..19655)
                     /locus_tag="VAH76_000755"
     CDS             complement(19365..19655)
                     /locus_tag="VAH76_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HEO1385403.1"
                     /translation="MRHSIVVLMPIISVLLTGCGPMNSNFSCNATAGDSCLTIEQVDA
                     MTRFADDIKPTKQSLGMMKAENNIPNRQGKIIKQNNGQSLWVARNMEEKSWA"
     gene            complement(19652..21025)
                     /locus_tag="VAH76_000756"
     CDS             complement(19652..21025)
                     /locus_tag="VAH76_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HEO1385404.1"
                     /translation="MANWQWNKMVKGRQVRTLIVVGCGLFFLFAMMIVLLADDRPKKN
                     LEAKKSVNLTGIIEESFTDAVADNALTAQQTELESLKKQVSELTLNIKSMAEAHQKEL
                     QAQKEELTSQVQELVLANQEKQSNSEQQDNPSELKEVQNPQLWHNPNVYSMNNTMNRG
                     TVLSDAIPRIHTVSFRPKRHITKKHHNFYLNPAHYVPSNTSVRAVILGGADADASVNG
                     QSKNNGVMLFKFLEDGTLPNGQRSRLRGCRVSANSYGDISSERAFATLYRLSCAHPGE
                     PIIDKEVTGWVFFNGKVGIKGKPLMRDNKVMTWAGVSGALSGIASAAQYAQSVQAIGP
                     YGATSVVPSSQIAPFAAYGGASKAADTLSQYYVKRAEQYHPVIQVGSGNVVTIVFKDG
                     FYLEPDEDKSQHAMQRYKAQEETQELISRGEEEKNSEMNFTVPPEVLGKINRVNTLSR
                     NETGGLQ"
     gene            complement(21018..21734)
                     /gene="traK"
                     /locus_tag="VAH76_000757"
     CDS             complement(21018..21734)
                     /gene="traK"
                     /locus_tag="VAH76_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HEO1385405.1"
                     /translation="MHKPFSIALALLSTVAMGANSIPVKDNSDIALTLSQGNYNRLVV
                     KNDKIMEAVFPPNAMAIKRDEQDGSVYVMLAAANPFTLFLTTESGRHFSVTVNGEESL
                     GKTIELVPVLPVVAASKTHAPITNPKKITQEAVPEAIIAMLTHMEQQKPFADVKVRRQ
                     FGRAEHWSKGLTLLPKESWDSKLLKGETIELYNGGKEPLELAQDWFAKEGTLAIKFSK
                     PSIKPGERAMLYRVEASHHG"
     gene            complement(21727..22296)
                     /gene="traE"
                     /locus_tag="VAH76_000758"
     CDS             complement(21727..22296)
                     /gene="traE"
                     /locus_tag="VAH76_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HEO1385406.1"
                     /translation="MDFKSYQSRLSQLSARFNLMVVLVFGLLISNVLLSCFLFKAWNQ
                     HTIEVTPFSGAPSYLKSASSVDGHYLSLMAENFVNERLSVTPETVDASHQRLLSYVGH
                     QNYNQMLRLLTNEAKVIKDKKMSSIFYIDKNKANPHELTVVVSGILKRFVGLQALNDE
                     RKTYVLQFQYKDSRLSIVQFAPLKEKQDA"
     gene            complement(22306..22602)
                     /gene="traL"
                     /locus_tag="VAH76_000759"
     CDS             complement(22306..22602)
                     /gene="traL"
                     /locus_tag="VAH76_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HEO1385407.1"
                     /translation="MSNMNYQFLNHIDAPKRVLTLTMDELVVTALSFILLILSNQKVL
                     VSMLGLGLLSGLRHLKKGEGPKVLLVLAYWYLPSYITQFFLPKLPLSHHRVYVA"
     gene            complement(22615..22902)
                     /locus_tag="VAH76_000760"
     CDS             complement(22615..22902)
                     /locus_tag="VAH76_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385408.1"
                     /translation="MKMGKKIVSLMQKAGSAFMFAMLSKSVFANDLLARALEGDVKDS
                     LGSGSMFWKIFILVDIILATAMAVKSKNPMVFIGVTAVAFIPAFLLKTFVF"
     gene            complement(23022..23768)
                     /locus_tag="VAH76_000761"
     CDS             complement(23022..23768)
                     /locus_tag="VAH76_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HEO1385409.1"
                     /translation="MIETMKSGLTKTLLASVVIVLTGCAATHTAIEHGSLQVSTKQSE
                     TIFLDPVPNTQKTVYVSVKNTSDEDVDIAPQLKTVLSAHGYKVVNSPSSAHYLLQANV
                     LKVGKMSVAASQSALGGGYGSAIAGAAAGSAAGSLTQSSTGMIVGGLAGGVIGMATDS
                     LVKDVNYTMITDVQISERVGKGIKINEQFQSHLKNGSSTVTSQTSSRDSQYQRYRTRV
                     VSNADKVNLKFQEARPSLEQGLVKVISGIF"
     gene            complement(23902..24060)
                     /locus_tag="VAH76_000762"
     CDS             complement(23902..24060)
                     /locus_tag="VAH76_000762"
                     /inference="COORDINATES: protein motif:HMM:NF014638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEO1385410.1"
                     /translation="MLILERRVGETVAIENKVFCMVLDHQLDGQLKLAFDAPECVPFH
                     RFEIQEGS"
     gene            complement(24117..24302)
                     /locus_tag="VAH76_000763"
     CDS             complement(24117..24302)
                     /locus_tag="VAH76_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385411.1"
                     /translation="MNAEQEVFDILEGCILCLQKAATEIGGFDFDKHAQAAVFAYSLL
                     KRQQLFLIYPFENETVH"
     gene            complement(24415..25449)
                     /locus_tag="VAH76_000764"
     CDS             complement(24415..25449)
                     /locus_tag="VAH76_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HEO1385412.1"
                     /translation="MYETVVFLETSLSHQEAMHLLPDAKYLPSIKKGDVLKSINDGYK
                     RIVIIDGNFSCVPSVWHKEILTALDYGLEVFGAASMGALRAAELDAFGMKGYGRIYEM
                     YKNEEIDGDDEVAIAYSKYNNEQTIPLINIRLTLERINITNKETILDSIRSIFYAERT
                     WGRIAQQVSEELYHLIKSNYADVKKEDAKSLLFYLNHQQILPMTLALGKKKRNFTLFE
                     KKLIEATLSPVWLNSHELEQIGEVAHLQRAENIVKLLSIPKTKANMLHYQSIIAMLDK
                     QEYEITECELIYQVELFREERNLLKGDEFVNWLKDRGLYDSNLEQLFYDYVKLSKYFA
                     ITYDYNSYFN"
     gene            complement(25442..26503)
                     /locus_tag="VAH76_000765"
     CDS             complement(25442..26503)
                     /locus_tag="VAH76_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEO1385413.1"
                     /translation="MIIRHAETSFRARHFSETLNLLNQFKKLAGITRLADLTHLDYTS
                     LPVYTAIRPRAKSLTTSQGKGLTKEAAKCSALMESIEVYFAEEIIPQVTNKSELELTQ
                     SNNLFIPINQLANSVRFTNPSQPINWVYADLVFSGKTILVPFAEYSLNSYLPEVLIYS
                     PDTTGLAGGNNYKEALLHGILEVIERQDAQQITEIAFVNSNLVENLSIRFDCFITYQE
                     NYYRVPSFEVLLKSKNPFENQILFKGSGSHLNKKIALNRALTEAIQSRVTTIAGSRDD
                     LINTKYDSKASEFPVVMNKKNFDEVQDYSVGTIEDALSVLYEKIKENNQDILVYTYYD
                     KEICIVKVKLISMDLISHV"
     gene            complement(26500..27522)
                     /locus_tag="VAH76_000766"
     CDS             complement(26500..27522)
                     /locus_tag="VAH76_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoeB/ThiF family adenylyltransferase"
                     /protein_id="HEO1385414.1"
                     /translation="MERYKRQIAVIGEQSQKILSNARIMIVGLGGIGCPVAQYLAAAG
                     VGKLILVDNDKVDLSNLHRQILFNEADVGDYKAEKAKVALSQVNCNIVLEAYTNKFDV
                     DFGYSSVSDVDLIIDGTDNFETRYLINDICVLQEKVFISCSIFVNVIQLVLFDTKHCC
                     YRCLYPNPPPTGAIPNCSEAGVLGTVTGIAGTMAANLALNYLLKVEDGEAPQVRIVDA
                     KNFSISSLTIKQNNECIACKQKKINFDYLQTSSADYGISIEDLDRNKHFLVDIRQKEE
                     REIIKLDDDLFFPIKDNNNYAFFLSYKDKKLVFYCASGYRSKLFASELRTLGVDAYYL
                     KTSLSK"
     gene            complement(27527..27778)
                     /locus_tag="VAH76_000767"
     CDS             complement(27527..27778)
                     /locus_tag="VAH76_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEO1385415.1"
                     /translation="MHEYQLIEFSSSKKEIQLVSNQSNLQQVLLEFYKEKFEKMFDED
                     GCSKGYISVFVNSKQITSVKEIALNPNDEICIVTSISGG"
     gene            complement(27926..29089)
                     /locus_tag="VAH76_000768"
     CDS             complement(27926..29089)
                     /locus_tag="VAH76_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEO1385416.1"
                     /translation="MRITTLLCIDPLQDYELIDCEMLLQLGITPFFLIKDASKAVKDR
                     ENVLVNSFSYEDILAICDSIRPKYIVCFSEDMFVDIAKIREHLKIAGMSSNTAMLLSH
                     KDLMYQKLDGSFPFPKTTTLIESPNLKSIQEKIGVQEIFVKPINSSGSYETYHIKTER
                     EFSNFLANKKEDEENYIAQAYVADDLYHSELVVFDGNILFVEARKYTHPNHLMVSKNF
                     PIFSLNILEAPQRQSIIDASVKVSQLLGFHNGVLHTEFFMNEDGYIQFIETNARPPGI
                     GLNKLYQRKYSISLETILCCIVCGVEPPSMVENKSHFVCGYFPTKMGVIKKINKPELH
                     VQNEWTFFVKPDDTSEQMAHMSKSAMVLCWDDSVTEINKTGNFLAEQDIVEVY"
     gene            complement(29061..30323)
                     /locus_tag="VAH76_000769"
     CDS             complement(29061..30323)
                     /locus_tag="VAH76_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385417.1"
                     /translation="MSELSWDRVKTDIARVNKRLYEILETIDGIQNMLFTVLEYQYGQ
                     IIADEDYFYLPNTCGKLSSVPFSMVLEKNLEMFIEFKGKSSTHQVYKEGDFLSVSSLY
                     NASNTHHPTDILQISSGARNTFLLCPVADARPHASLEKYFKADIPVPDELGNHYLTFK
                     SLCEAANCKWRSKLLVFPSEIVTLIKENKLPTLLSLVMEFDSNQTGYYANLPFYNYLM
                     AYVRANNPEISQNEFTNNAISQLITIGTGQVPGYGLAVGDDLLPLDFIVETYRDIYKS
                     KYTPFVMEPVHFDKNKNNPVFYSILKEEMAFKPTSFSNKPQRCELIYDTYFQYADHIK
                     KLGHLKNTPFFESATMLDLTLFHEKNNQVTNNLFKLPRETIFSYDPRFLEVTNKLGYS
                     VDQFPSKTTFLVGCFGIKYNENNDTVMH"
     gene            30722..>31132
                     /locus_tag="VAH76_000770"
     CDS             30722..>31132
                     /locus_tag="VAH76_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385418.1"
                     /translation="MNNSIYYHRFNKLLSLTNCPKEALLLDKIIFHYQGTLLKRENKL
                     WFTKKIPDLAAELGFSESRIYIYLKNLEEEGLIIRKRFKYYGVPRSFIAITETLQSKL
                     QFLSKEKENTIEIKEKKPTSRNDINERMDYLVSAD"
BASE COUNT         9319 a         6549 c         5648 g         9616 t
ORIGIN      
        1 aatttttgat ttgttcagtg aatattggtc acctaaaata gttggtgagg cgaatgattt
       61 gcaaattaaa caagtaaaat ttaaaggcga gcttaaatgg cataagcacg ataatcaaga
      121 tgagttattt caagttgtaa atggtaatct attaataaga tcgagggata agggtattaa
      181 cctcatagta gatgaaattc tatcaagtta tttcccagta ttcttttgat gcttaattag
      241 atatcacaag gtctcattta gtgatatgaa agccttttct tgcaaatgtg tgtatagtgt
      301 gtataatata aaatatgaaa agcgcagaaa taataaaaat aattgaacgc gatggttggg
      361 tgcaatgtca tcagaaagga agtcattgcc aatttaaaca tcccaccaaa aagggtaggg
      421 tgacagtacc tcatcctaaa aaagatttac ctataaaaac agtggccagt atctttaaac
      481 aagctggtat taataaggag gatataaaat gagatatcct gtagtaatac ataaagacga
      541 acatagtgat tttggtgtaa ttgtccctga tattcctgga tgctattctt ctggtagcac
      601 ttatgatgaa gctttaacta acgtaattga ggcaattgaa tgccatttgg aaggattatt
      661 aatagataat gagcctcttc cagttggtac aacaattgac cgatggatta atgatgaaga
      721 gtttcagggc ggggtatggg catttgttga cattgattta agccaaatat cgggtaaagc
      781 aaaacgcatt aatatcacta tgccagagcg cattttaagc ctcatcgatc tgtatgccaa
      841 aaaccatgcg ataaaaaatc gttcctcctt tttggccgat gcagctctga gttatatgga
      901 aagtcataag taatgaaaag tgtgctgtat aggcttcgtt tcaattaatt gggttaaatt
      961 acagaaacat caattcagtt tatgtttatc ccatatctat aatttaaccc aaaaatcatg
     1021 gtcgatgact cttccaaaga tggttaacat catcaatacc tgttcatgag ctataatcct
     1081 ttatttctgc attggaagtc tttgcattta aaacttcttt ttaaactatt aagtagcatg
     1141 ctatacaaca tcactaattg aatcaatcac aaacgcgcaa tcgtaatgcg ctacttaagc
     1201 agtgaataaa agagtaaaaa ccaaacgccc aataaataaa caaaacatcc agttctaaaa
     1261 gcagagacac acctttcgca tcattaaaat atgcgttata agtcctttat ttataggtaa
     1321 ttttggttat ttttattcat ttttttacct aaaaagatgg tgaatagctg gtgaagggta
     1381 atggtgaagt aaaataattg aaagttcttt atttttcatg gcctctaaca tcaacttgta
     1441 taatcttcac catctcttgc accataggtt caccatttta tcttctaaag attttcatgc
     1501 gcactgtttt taagttcgct atatttaata gccgcagcct gctgtagctt ctcaaaatcc
     1561 tctctatcca atttcaacaa ctcaattgac atatttttaa taagcgcatt catctgaata
     1621 attagttgat ggcagtactt ttcgacagct ggatttgctt tgaagtttcc gtcattttta
     1681 aaccagtgat gtaatccaac ttgaattaat tgattgatca tattggacat agaatcatta
     1741 ttttttatag ctaatgctga tatgtgttga tgcaaattat ctggcaatga aatggttaaa
     1801 cgtgacatat aaactcctcc tatcttatag ttctaattct ttacttaatt gcttggttaa
     1861 atgaggtcct ttcttctcaa taatgagatc atcttgattc attgatttag atatttcttg
     1921 aatttcaagg gattttttct tatcaattac tgattgtatg gataattgat tttgcgcccc
     1981 gaatcgacgc tcatgatgaa ttaattgtgc gcttgaggta atatccattg aggctggttt
     2041 ttcgttcgaa tttcttcgaa tagcatgaac taattcttct ttattatccg ttaccaaagt
     2101 cataccatga attgctctgg aaatttggac gtaaaaattg tttaaggtac tgccaaatcg
     2161 gtgaaatgat tccattaaac caatgccata gggtgcatct ttcccttgaa ccttgtagtt
     2221 agtaagaaca tagctataat caagatgttt taaggcggga tgggattttt cgagtgttaa
     2281 taaacgccct tctttcgtga taaacataaa agagttgttt tttatttcat gaagagtcgc
     2341 acattgacca tttcgtatat cgttagcttt aaaatttcgc gtccacatga ccttatcacc
     2401 aaccaaaagc tccaaggatt ttgtttcata tacttcaatg gttcgttcaa aaggggctgt
     2461 atgagttttg tagtttggca gattgtttag attaaatttg attaaattac cactctcatc
     2521 aatcagtggt aacacattat cttgacgatg ctttttacta atttctccta ccgtgtaata
     2581 actaccagcg tgaattttat tccttttaaa actttggtta aaacgcacca tatcgccttt
     2641 ctggtaatag gccacaaatc ggctctggat aggttccatc atttttgttt taagaataat
     2701 ttgactaaac tgctcgcctt ttaaggtccc ctcttgtttt aatccactgc gaataaatgt
     2761 ggtaatttct tctctattgg cgtgtgttgg cgcaaatagc aacgtttcat ctcgcgcgga
     2821 tgcggataaa gaaagccaat gattggcaat ccaagcaata cgctctttat gagtcgcaag
     2881 cgttttaaca tccaaattat ctaatgcttc ttgcacaaaa cctttcgtgg ttgctattac
     2941 agcttgcctt aagatctcgt ttttttgccg aacaacttca tccatgacag cggtttcaat
     3001 accatattct tgggttaacc caaagattcg ccctgcattc acactgggta gctgagctct
     3061 atccccaact aatacgagcc gtgcaccaac acgctcaatt tgcttcatca actcatggcc
     3121 ttggtgagaa gaaagcatgg atgcttcatc gataataaat agtgttttag ttaaggaagc
     3181 tgtttgtgct tctttcaatt cctgatgcac caaggggaat acatcagctt taatccctgc
     3241 tttttcttgc aactcatgtg ctgcagagct tgcaaccgta atcccacgga gcttatgccc
     3301 ttgagcttcg ataaaaattc tcgcctctga aagcatagaa gttttggcaa ctcctgcata
     3361 accttgaata gccaaatatc gatctttgct cgttaacaac accttcatgg attctttttg
     3421 tgaattggtc atggggtaag gtaacaaaga gctgcgcatt ttctggaaag ctgtaactgc
     3481 attaaggtgg gcaatagcgg gaactgcacc tttattgcga tcaattctgg ctatagtttc
     3541 tgtttccaaa gttaaaagcc aaggtgttgt gagcaagcgt tctccacgat cgaagcatct
     3601 tgcttcatat agcgtttgac tttttatttc ttgttggata gattcaataa ttgattgcct
     3661 tgaaaccgct tttggataaa taagactatg tttcatcgct tcttgagtta acattctttc
     3721 tgaaaaaatt gaagttcgct gacttaacgt ttctattgcg acgacaacac aagcgtgtgc
     3781 tgcttctatc tctgatgggc tagaatcttt tttatatttg tttactaaag ccaataatct
     3841 atctttaacc cctgagagcc aagaaataga ttgcgattta tctcgatgtt gcataaacgc
     3901 ttgagcatca aaccctatat cttttgcgcg ttgtttccaa tcttctgtaa ggagattgat
     3961 atcatgttct tctttattct tgcgagtcag tacagtagca gtggatgcac tttttgcacc
     4021 attccaaccc ttctcatcca tataccccaa aatatccatt cgccttgttg aaaattcatg
     4081 taaaacctca tcaggaacac catcaatttc aaacaaacct tttcctgtta atcgcaaatg
     4141 aaagcccgtt tccttaagct tattggccaa atgttgacga taaattaaac cacaatagtg
     4201 agcattattt tgaatctgct cgattacccc atgttctctt gaaggatcac ttgacaatga
     4261 tcgcgcttta ccttcatgaa atgtaatatt catggtgaca cagtgggtat gtaatgctgg
     4321 atcattagca cgagaacttg gctgtctgaa tgctgcaacc aataagttat tggtcttctc
     4381 aaaagaaatt tcaccccgac gggaaaccct ggtttctgca aattcctttt caatttgtcg
     4441 gatagtcaat tgcaccgcct catcatgaaa ccgtactaat tcaggtgcta aacctaatcc
     4501 tactaaaact gacactgatt taggagcaga aaaggtcata tcaaaaccag gccgatgttc
     4561 tccttcaaga ttttgtaaaa cttggccatc tggcaatcta ccttctaata aagccagcat
     4621 ctgttctttt tgcacaacac cagagagctt caatttttga ctaccctgcc ctagccactg
     4681 catggctgtg gcatcgcctg aataataatt gaatgcttta gagtaataat cagcagcacc
     4741 ttgagctgat ttaagcggtt ctacactgag catgcctatt ccttattcta aacttcaaat
     4801 tctatttcca cggatttttt gttatggcat ttgcgcttcg caatagcttc atcctctgtg
     4861 ctctcagttt ctttaacgag cttttgtagc atttcatcaa taatgaattg gcgctcaaca
     4921 aaccctttca aatatttagg ccgattctca agttcaagcg tgagttgagt aatgggataa
     4981 cttcctggaa gacgcacaaa acatcttaga tcttccagtt ccaaaatttg tggataggat
     5041 acaatgggac gtgtcacacg ctggctacca agagatattc catctcgaat actattagct
     5101 ccataggaat aattttctcg tgattcttca atttcttctt ctcccagctc gctggcaacc
     5161 aaacgagcca tatcagcgct tgggcttcta aagaaaaaac gggtgttcag caagtcaaac
     5221 aattccttag caccgcctga ttgaccatac actttggtga gctgagagaa actttgcatt
     5281 cctaataaaa agcaaccacc aaattttcgg acttctgcaa tggtctcacc taaaagcggt
     5341 aatttatgaa gacttggcaa ttcatcacaa atgaaccaaa tccggcgatc tggattaggc
     5401 gtaagactta acaatgtgag ggaagccatg gctaaccaca tagacattaa aggctttaat
     5461 gatttgtgtt gttcgccatt ggatgaaata aaaagccaac cactgtgctg ttcatttaaa
     5521 atgtagtcac gaatagaaaa tgggcttgcc ccttctgaat caagtcctga taaggtagaa
     5581 agcgatttta aataagtggt aataactgaa cgaatggaaa tagccgtttt ttcaatttta
     5641 ccgctaacta gagttgcagc agctgttcct ttaagataat cctctaaatg agaaaactca
     5701 ccggtcaaca gcaacttaag caatttttca acggatcgat ctttatcttt tcgcatttta
     5761 gcagcaaccg aagaaaatac agttctcgcg gcattcaccc agaagggctc agaatcgcca
     5821 tgcatcggaa taaggctttc tgccatgttt tcgaaatcag aatcccgagg tgcttcaagc
     5881 cacatatccc aattaggaca cctcgcatca aagggattta gaatgacatc ctgatttgaa
     5941 tcaaaataat gcggaataaa ggcacatccc ttatcataga caataactct atctcccctc
     6001 ttgcgtaagg catccatgat tttcataatg agctgacttt ttccagtacc tacagttcca
     6061 tgcaccaaca gatgttgaac ttcgctgttc gctttgaggg gaaacccgtc aatagtgata
     6121 tcagagtttt cttttttgtc caggatttgt tggattacat tttcctttgt atcaatacgg
     6181 ctgccacgaa taaactgatt agcacgctgc gcttttcccc tgtggataaa ataaaaggca
     6241 aagcaaagtc ccaaaacaaa tgaaaatacg aaagctgcca atgaagattt tcctaataga
     6301 tctaccatat gatcggcatt ctctttgtaa taaggataat gcagaattgt ctccacgttt
     6361 tgttgatagt tttttccgtg aaagggaagc gtgaaggtat gcttttgccc aacgagattt
     6421 aagaaacttg catagtaata agcaatggct tggtggaatt tttcgggaga tataaccaac
     6481 caggtaatta agccggttaa tacgacccaa agaaaaagac aaacccatac caacgtttta
     6541 gtaatttgat cccacatccg cagattgtga aaactgattt gcccgccacg agtatagtgt
     6601 ttgaaattcg gctcttcacg cacaatattt actccttatc ctctttttta ggcaaagcag
     6661 tagctaccat tgcctgtaaa atggttaaaa tttattcttt ttgcgtttta ttttttattt
     6721 ctgggcttct tctattgaga cagtttttcc tttagaaaag aattcattac atagagtaaa
     6781 taggctgcta aagccaattc ggcttaattc catatggacc gcaatatgag tcgctcctaa
     6841 actcaggaag aatgacacta ccaacataaa aaaaatctgc tgtccagaag cataactgcc
     6901 aaacaaaata cacaagaatg caaacatgcc aaaaaacacc attgaaatgt ggttacattt
     6961 ttttcttatt aatgcactag tctttaattt tttctccaga tgaaactgaa attgaacaaa
     7021 aaaactaatg ggagttagca agataagcat cgcaacgatg cttaagaata gagcggtaat
     7081 gcctgaatca ataaacaagg aggaaaaaga gatcggctcc ttgtcatcac tcataacata
     7141 gcatagcgtt aaaaaaagga ttatagacat ccgccatacc ccattaaaaa ctccttttaa
     7201 gagccctccg taataaaaac gggcaggaat aatctctggg tttagcattt taatagtttt
     7261 catgtcctct gctatggctt gctttaattg ttttggatcc actctactct ccttttttat
     7321 gtaatttctt gtcctcaaaa ggcaggtgca tattctctgg cactactaca tttttttgag
     7381 cattcatcat gccttcttgt aacctcaaat ctttttcatt cattagatca cggctccctg
     7441 acttaatcaa ttgctctcct tgttttagat tttgaggcaa agtactcttt tgttgatgca
     7501 cctcttgatg aaatactcca agctctttat catttatgcc aaccttcatt gattgtgcct
     7561 gatgaagaaa ctcttcgctg tttttttgat aatgggatcc taattggttt tctttggcaa
     7621 taaagccgct tttttctttt tgataaaacg attctatctg tgatttatgt tctgtattac
     7681 cgaattgggc aattaactcg tctcgtttgg atgcaataaa gtcatggcct aatgcctgaa
     7741 gtttattaag cgctcgcaga tcccctgggt gactatacaa ttcatcgcgc tctgactcat
     7801 taagcctagc agctacataa gcttgaaatg cttgatttaa atcagtggta atttgagatg
     7861 aatgagattc cacgaagttt ttggcattct gaatgcgtgt ggctttggca agggaagcgt
     7921 ccacattacg gctggctgtt tctgcctctc tcagatcagc acccaattga ttagacaatg
     7981 aagccgcttg tgaatgacta tcatcaaaat gatgattttg agaaaactgt ttcacataac
     8041 ttatcgcgtc attaaaatca cgcgattcac gagacgaaag ggaaccgtcc ataccggtgt
     8101 gatatttatc ttgacttgta gaattacgat cgaatttaag atgcgcatca gcgcttgcat
     8161 gaaaaccagt tccccattgt gcaagcttac cccatatact accctggctt ttaaaaccaa
     8221 cttgcgcact taaaccaccg cttgtcaaat gtgttagtgc gtcttcttta ctaacaccta
     8281 agcgtcgcgc cacatcctct gcaatatggc taatggttga taaggctttg gagtattgac
     8341 ctgtctcact ttccgatatg ctatctccca aacgcatgtc atgccctgcc aattgtgata
     8401 attgcagggc tcgatgggca aaatttgata atgaattctg atagtgtgca gattcgtttc
     8461 gagcagcttg tgtcgattgc tcataagcct ggttcagtga tgcgcttaaa ccttccagtg
     8521 catttataga tacagcactt ctcgtcattc ctggactgac atcaaacact ctatcaccgc
     8581 tgcccgtaat ggtttttaac acaccacttc ccaattggga agtccgcatg ccgtgcaggt
     8641 gagtccaatt ggaatcgtgt ttattggctg agaaattatt agcagtagtg ttatagaaac
     8701 tggtttgacc aagaccaaaa gaggcgctgg ctgcctccgc tgccactgac atgctggagc
     8761 cttgcatatg actcatcatg ctggttgcga gattactgaa tgcgccgcct agattagaaa
     8821 ccaaaccatg cgagagaaaa ggaataagca tcatcaaata gccacatgct cctgagatat
     8881 ccgcatgcaa ctcatcaatc ttatcaagat tcaccatcgt aaattgacca tattcacccg
     8941 atgaggaagc gccataaatg gtcattcctg cgttgagtat tgcaaagagc actggccaaa
     9001 attgcagcga cataaaaaac tgcatatagc cataaagaat tcgtatcccg ccaggtaatg
     9061 tggttaatgc aataatcaac ggaaatacac caaataatat aagtgttaag caggtgtggg
     9121 taatcggcaa aatccataag gctttttgcc ctaatacttc ccagctccag cggtgttgta
     9181 cctgggattt tgtaaattgt tggttcacaa ttcctgcggt tgcatcggta aacgcctgat
     9241 aatgcgccac gccatcgcct atggcgttaa tcatcatgct ttgtaaaaaa atgttactgg
     9301 atgagtcggt taatccctgg taatactcaa aggctgattt aagatgagta gcaaataacg
     9361 cttcataagt ggtattctta ggctttccaa acaaattcac gccaaaaaat gtgtaggctt
     9421 ttttaatttc agcatccagc tttttgcgca aactgtactg cccatcaggt tttgaggcct
     9481 ccaagcatga aactaatttg ccattcacag agagcatacg aagcggtgaa gctttcacag
     9541 taataagatt ccaaatgtcc gtggaatgcg ctaaatcact gacactgtac tttcgattta
     9601 atcgaatgtc tcctacgaca cagatacgga aatactcatt catttcttct ttaagcacgg
     9661 gatctttaat acgaaatgag gttgatgctg aaaccagacg ggaaccaaaa agagctcctg
     9721 ttttggtgta ttgcaaatca ttgggcatgg taagtaatgc atcgtaggat tgagccaaac
     9781 catagccaat ggttgtgact aaactggagc ttaacgcaaa taccacgggt acattatcta
     9841 cgagttttgg tgtttgagaa gaaatatcgt caatcattac ggaggtttta ggtaatatca
     9901 ccaaattcac aaacagcaca taacctaaaa accaccgagc aaatgccatc ggatcacggg
     9961 ttttaataaa accgatggtg gccatgacga ttccaattaa tgcggtaatt cgtaaaagcc
    10021 ctaaaaaact gtcctgcttc ataaaggcgc taatggcatt tagtacgtgt tggaataatt
    10081 cacctccagc caagacatga attgttatca tctattgtct ccttaacccg cagtactgtt
    10141 catttgagaa gagacctgtt tttcaatttg cgccatacgc tcaatcaggt tgagtttttc
    10201 ttgtagtttg cgacctacct ttggatcaag tgattccacg tgtgtggttg cttcattaat
    10261 gcgcttttga atttctttga ccaaatcggt atttaattca gaccctgctg tggctacact
    10321 gacctcacgc agtaattcga ttaggtaatt agttaacaag tcctgggcta tcagcattga
    10381 atactcttct atgtctaccg cactactgcc atagtgcatg ctgtttaata ccgacaaaaa
    10441 cttcatcaca ggcagcgatg tcatacttaa aaaattaagc tcacgattac caggtttttc
    10501 gtcattaatg agtttggtat tgatggtgcg aataatctcg cgtactttat aggtaagcgt
    10561 tgaagtttct tgaatggtga tatcttgcaa actcacctgc atacattggc tattgctgcc
    10621 cgcatccttg caacgccata tctttgcaga tctcgggcca tttccagtac caattagcgc
    10681 attcactaaa tcagcatttc cagcaaggga aggtacgttg gttaccttac cttctttgtc
    10741 aataatcagt gtcccagtta aactcatcac catttcagcc aactctctgt cactgtttaa
    10801 gaatgatttg gcttgcaaca gtgaccatac aagattttta tttaaaacca cctgcttttt
    10861 acgctcagga tcttgagatg ctttgctcat cacattatca aagccagtac ctgcgcaaac
    10921 cattcgcgcc ttcacataat cactgaacaa tccctctttt cccatggccg cctggtcttt
    10981 acagattttt tgttgacttt ctgcggtttt tggccaaatg cctcccacca tgttttgtga
    11041 gagctgacag gagttaatgc tggcctggtt agccatttgt tccaattgct gcaaataatc
    11101 ccttacctgt ttcagttcag gaaccgtaga ggcaagcatc acatccactg cataagcacc
    11161 agcattggtc atcaccgatt ttcccaaatc aacaagcttt tgctcactta aaaaactcat
    11221 actgccagta tataaatcaa tgcctccaca tccagcacgg taactgggca aatccaattg
    11281 caccaactga tactgacgta cctgattacg agcatagagt gaacccccac caaaaaatcc
    11341 tgcagcttga gattcaaagg catcgggcga agtcacatta gacgcatagc ccatcccgtt
    11401 aaagaaatta tcgagatctt gacttacatt agcctgaaca acagggaaaa aagcccataa
    11461 aaacagagtt ataacggctt ttttcattac agtctcccct tgatttcata cgctttgatt
    11521 tttgaaatga gtacagccat tcggtcgttc agttcagcgt tcgtcatgga accaaaaccc
    11581 actggatata atgtttttgt tttagggtta accacaaaaa gtgccggata attaatggaa
    11641 ttaccaccaa atgccgcatt aatccactct gtagttgccg gtaaaaattt tggaaattca
    11701 ggcaatggtt gattatccaa cgacaaaggc aagacttctg ctttgttgcg atttgcccac
    11761 ccttttaata tcggtgcaaa ccgcttgcaa tgtgggcatt ggcttccata aaacaaaatc
    11821 aatccatggg tgctaaaaaa tccctttctt tttgtatttt gtggctcttc tcgttggtgc
    11881 gttactaaac cttcttgttc ggcaatcagg ttagttagcc attgagatcc ttcggcctta
    11941 gcacccaagc caataaaagc gagcatcaca atcaaaaata cacgggcaaa cattattcct
    12001 ctcctttaaa ttggttgacc acttgtttta tacgttccat caagacatcc tgcgtagtta
    12061 agccatatgc cacaggtgaa acctgttggc ttgcaggatt caccaacagc agtgctggaa
    12121 aataacgaac acccaatcga ttagcttgcc cttgatccat tcgagaattc agcagttcgg
    12181 gagaggttgc gccatcaaca ctaactggga tcagttgaat gcgaaatcgc ttgcaaaaat
    12241 caacaataat gggtatttgc tttaagtcat aagggctttt tccacgataa aaaaacaaca
    12301 agccatgtga tttggacaag cggtttataa cttctatggt ttgggattcg cgcacctcat
    12361 cggtgatttt tgcgcccaga ttagaagtcg gatgagtcac tgcataatca tattctggat
    12421 gtgccaacat cgttttctga aacaatgact taaatcggga ggactcttta agccagtaat
    12481 cttgcagact taaaaatacg cgcatgtcct ccatcttttt ggtgtaccgt gctttatgaa
    12541 gcgcttccat ggtgtagaag cgcagaacag catcgcgctc tgtatagctt agactcttaa
    12601 aaggaatacc cgaggttttt ttctcgactc tttttgtttc cctttctttt gtataccata
    12661 gaaaacccac cggtttatca gcaaacgcaa ccccactaag tagcaaaaaa acaattagca
    12721 tcctattaat catgagcacg cccctgttgc tttaatcgtt caatatggga ttggttggtc
    12781 tgtttaagag aatcattctt aggcaagacc atgcgagcca ccagctcttc ctggatgggt
    12841 gataaatcca atgaggcgaa attaatgcgt tcgagttctt caggggtaag tcctcgacaa
    12901 tcaggatact gtgctgttcc atacggaata cctaattgcc ccccacgccc ctgaatttga
    12961 acaattgatg ctaatttagt aggaaatatg caaaaggtat aatgtcgagc acctaattta
    13021 tggtttttat aagagcctaa atacaccaca cggttttcat gttttgcacg ttgaagcacc
    13081 tgcagtgctt ccggacaatg ttttacccaa tcaccccatc ctgaatcagt acaacaatcg
    13141 cgaaaaccca gaggatattt tttacattct tgcgcacttc cgttaaaaat tgcaggagtt
    13201 ccgctcctaa cctggtttgc agtcacgtct ccagccacgc cggcgagtag cccaagcctt
    13261 gatatacctt ctgccatgtc atcactggtt tcatctcttg aggtgtcgca ctgtccatcg
    13321 atgcaataca tttttcccgg gcatgcagtt ttttctggca tacaaaattg ctgcatacat
    13381 tggaatgttt gggaataacg ctcacagcga ttagcttttg actttacaca ggtggatgca
    13441 gtttgcgagc aaccctgatt aatgtactca gtacaagtgg tggattgaac tgatccacat
    13501 tgatactgta atgccctacc ccaacaaggt cgtttaatag caactcctcc aatcactttc
    13561 acctgattag gctctaaaca cgcttgactc tcttccaaaa aacaatagcc attcaggttc
    13621 tctgattgaa taggttcaca aacctgtgtt tgccattggt catcgctcac gtattctgtg
    13681 actgttacct gcaatggaac agacagcctt ccattgcctg aaagttgcac tgtaacagtt
    13741 gtgtcggtac aagtcggttg tttagtcaca cgcaaaggtt ggccgtggtg tgtgacatga
    13801 acagaaatct cttcacaagg ctgtgtcaga ataacggtat tagccggtga gcactgccta
    13861 tcagctgggg aacaagctgt taaatcaaat gttgtcactg acttatatcg ttgtggcacc
    13921 acagttcgag aaaagctcat actgagtgat tttactgtca cactcaatgt catctctttg
    13981 caagaaacca tggagtactg cagggattct tcgcaggatt taactgttga ttcgattttg
    14041 cagtggcttg cctctttata gcaggtacct tccaagacgc catctggatc accaagaatt
    14101 gtttcagcat actgcatttc aggagtgttg ggattcgcac gcactcgaga tctgtttaag
    14161 gccttattat aaacctcatg ggcagtcgag ttgtttttaa gtgcatttaa tccaagagaa
    14221 gcaaaaggat tgcttccttc ttgcggttga agcgaagttt cttgaggatt tattgtgtag
    14281 ccttttaatg cagttgctgg attaaatcct tttaagtttt gcaaggcttc atcacgtgca
    14341 tgaagtgcat caacctggtt ttgagaaaaa gaagagaaag ataggatgca gaagaaaatt
    14401 agtccgactt tactcaccga ttaccctcct gatatccctt ggtgtaaaat caacatcccc
    14461 tcttcctgcc atacgagcta atccttcttg aatgctcaaa tggccataaa tcacatcaaa
    14521 ggtctgacca tcatcaacaa ttacagcagg tacttgctga attccgaaac gctcaaacag
    14581 agtggggtca attgccaagt tcaaattagg aatatgcttg cttaatgcca tcaccttcag
    14641 tgccgtctca ctcatcgagt cacgataaag tccgtttaaa taggctggaa tgtttaattg
    14701 agcgctctct tttaatgtct cttgcaaaag agtttccggt atggaaaagc taaccaatac
    14761 agatatttgt gctgcaaagg atgaggtcat gattacagac ccaatcaata ccaaaattcc
    14821 ttttcttaac ataacaaact cctgcttaaa gaaacgtaca gtttcgtttt cgccacacca
    14881 aaaacccata ttggtcaggt gtatgcggct tgatttttcc ggcttcccag gctagagtgc
    14941 ttgaaccccc tggataacaa cgcttgccat cagccacctg attcaccagc tcatagcgat
    15001 aacgtgattt aggggttacg gtgtaataat gctctttgca aattgcatca gatttagggc
    15061 ttgagtcaga cactaaaaac tcacggtgca ttttgtaatt catgcgctca gtcagaagcc
    15121 acgctgtttg cacgggtgat acagacgcat taacgtgtcc tgtcatcgga taatgcgttc
    15181 cctgactgcc agcacaccaa aaaaggctat ccagtggttt actagcgatg gtagatgaga
    15241 gtgcatccgc agcacacgca cttgcggcca cagggttgcc aaacaatact gactcaggac
    15301 ttaacacaaa gctcatctct gaatcagacc atgtcgggtc aagttcggtc agataagcaa
    15361 tgtcaaaatc acctttttgc aggcaacctg aagaggtaat caaattaagc cagttaatga
    15421 gcggatattt ataccaatgc acatgaaaaa atgctctctg ctgcccatta ccaaccacat
    15481 ggcgtccacc tcttccctgc tttaatccca gatttaattg agtgccacca agattcacca
    15541 gacaataagg tgtatccgtg acatcggtaa gcgccatcgg ctcccaaaat ccgatattca
    15601 agcccaagcg aacaccggtg ctggctggac aaacgcctat agggctggat gcattttcag
    15661 tatcaggaag tgatgaattc acaacctttg cgttgccaat agataagggg aataaacatc
    15721 gccagcatac atcagtaata gggttcacaa aatgtcctgt acaaagcttc gcagaagatt
    15781 gataggacac aaggtacaaa acagttagaa gcagtactct aaagtacatc gccattctcc
    15841 ttgattgctt gttcatgaat gacaaggctg ttttgatgac gtatcacctt tgctggaacg
    15901 tgaccaatgt gcagtttgct ggtaattttc cctgcctggt cgaaataaat caccgagtcc
    15961 aacctttttt cagctttatt gacggcaccg ccagtcagaa tcaatttagg gtttgcacac
    16021 tgagtttttt gatgatctgc ccaatttaac tgagcatcat catcagcatt aaagaataac
    16081 caacacggtg tataggaagg catatgttgt aacgcattca catgagttcc ttttgaatac
    16141 agaatctggc ctttttcatt ggttatgtct tggcttaagg taatctcagg aacgtactcg
    16201 tgtttaattg aatggtgtgt gcgcacaatg ttcaatgctg gtggcctatt cgcatgctcg
    16261 gctgccgttt gaatccagcg ctgattcaaa gacttaagct caccacttgc cttcaatgag
    16321 gctaaccgtt cttcaatgag ccttaaaaag ctcttttcag caataggaaa cacttcaccg
    16381 actacaccaa aggatttggc gtgaacttgc cccattgaaa tcataagaag aaacaagtaa
    16441 aaaataatgt tcacaatttg ttcctcatgg cgtggctcat cttaattcgc atctcatccg
    16501 taatatcaac acctccggca agaacatcag acgcccttaa aatgatgact ttcttttgct
    16561 gtgctgtatc aaccaatacc ttattcagta atgcattaaa tcttcttgtt gattgctcca
    16621 cttgggactc tgtcgctttt gcttcggcca attggcgaat gaattgactt aagacttcct
    16681 ttttatcaaa aataacaatc gatttttgag gtgatacacc ataaaaaccc aaaaccacac
    16741 caaaaaccgc taatgcacaa cctaaaaata gaggagttaa tcgcataatt cctccccata
    16801 atatttgttt gcaacgcgtt ttattgcctc acttaagctg accccttgtt tggttaacga
    16861 ctcaatgaga cttacttcat cccctgaggt tgaaaacagc acgcgggaaa agggatcgca
    16921 aaaataacga tgaaacgtgg tgacatttcc aaactgaatc atcagattgg aaaacccttg
    16981 agtttttgcc tcaccaaaag attcgatgac acgtgcttca agcggactaa aatgctttgg
    17041 atgttcctct aggtattgct taaacgaacc ctggcgcata atgattttag tatcactact
    17101 tgccgcaata gcacgtccct gaatagtgtt catggtgtct aaaaggtttt gtgtgatgac
    17161 tgcaaaacca cccatatgct ttcttgcagt acgaaagccc tgctcgataa agctggcaca
    17221 cactggatta gaaccacgag ccaaaaaacg ccaggcttca tcaatgatac atcgtttttc
    17281 gcgccttcta tccgagtgat aaaattgccc ctggataatc acgatcatga caaacatgac
    17341 aatggtgagc agttcagggt tagattccag ctcacccatt tcaagaacca ccaaattgga
    17401 accattgagt agaggtgtat cgctgttaaa gaggtggccg taaatgccct gacttccata
    17461 tttactcatc aaaatcagca aatccttaag ccgttggtca ccttgggatt gaggtttttc
    17521 tagcatcctt tgaagtgagg ttaaaacatc atccattttt gcgtgacggc cccgcgcttt
    17581 ccagcattca gtcacagcat ctaaaagcca tgctttttga acttccccta atgcctctga
    17641 cgggcttgcc atgattgcta acaaatctct gatttgaatg taatcgttga cttgttcccc
    17701 tttaatctcg gtgaccccat caaaatcaaa cagcgtaaat ggattaagcg tcaaagtgga
    17761 ggcatcaatg tagctgccac caaccatctc gcataaatgc ttgtaagagc cgcctaaatc
    17821 aatgacgaaa atttgttggc ctcgcgataa accatccaaa atttgtgctt gctcaaagaa
    17881 agatttacca gctcctgtgg aagccactgt gagtcgatta aaattagtta tgggcaaaac
    17941 cctgtcatca aaggtattta aataaaacag ttgatggcga tacgtaggta caatgagccc
    18001 ctgtcttgct cctttaaaat cagcaatgac cggcagaaga ttagcaacat tggcgtgact
    18061 cagttttttg gtcatgccca agagctcaag actcgaaaaa aggccggaag tacatataaa
    18121 aggaagggaa cctaaaaatc ttaaccattg tttgcaacgg ctctgagtta atgtaaaacc
    18181 catttgccta aaacttgcaa ttgccttggc aacatgctca cgttcgtggt cgctagttgt
    18241 ataaagaatg acattataaa aggtaggtaa aaggtgcaat tcgccttttg atgcatgatc
    18301 gtgcacaaat tgccactcgg ctgcttcctc acgaatggat gggttaataa aggattgaac
    18361 gggattatta ttcgcgttta aggatttggc acgagcacgt gctttcgcct tcatcttttc
    18421 ctgattcata cctcgaatgg tgaatgaaat taaaaaagga cattggatcc cctgctcagt
    18481 atcaagcaag ttggcaaata aatccggtgt ttgccataaa gcaaatggct tggctgggta
    18541 gtgactcacc tcacaattaa ccagtcgagt tcgcactgtt tgtgcttgag agtcagtaat
    18601 tgatacctcg atagcctcat ctccaatttc aatcacggtt gagggacagg gtatagcttc
    18661 tgcaataaac ccatctgatt cattaatgat tggccaggac aactcattga aatcaggcga
    18721 aattaatgcg cgcatcagca ccttaaattc atcttcccca caccgcctaa ttccaaaacc
    18781 cgcgacttta agttcggatt caaagtcggc tagcaacagt tggagctgcc cttccacatc
    18841 agattgcatc ggtcttgaaa caaaaagata accacaataa tcacttaagc ccgccggcac
    18901 attacggcca tttttatatc cttttttaat ggcattcaga tggtatttca gcgtctcccg
    18961 tgcaagctca gcataaatac caccttgttt taaaataggc tcatagtttt gcgcaagttg
    19021 ttctccaatc catgggtgtt tatacaacaa caccgtgcaa tccgtcccta cagttaattt
    19081 atttttaaac agttgcgaca ggctgttcat taaggactca tccgcacccg acatgggcat
    19141 taacaacaag ccaaagccca ttgagttgcg attcacaaaa aaaccggctt cattcaccgt
    19201 ttcatagggt aaaaggcttt tgatagaggg caaatctaaa gtacacgact catatgcttt
    19261 atcataggag caaagagaat caggtttcac tcctgaagtt tctccaagta gatgacttgt
    19321 acgatgcaat aaatctcgcg ctttatcgcg ccaaagagag aaatttacgc ccatgatttc
    19381 tcctccatgt ttcgtgcaac ccataaagat tgcccgttat tttgtttgat tattttccct
    19441 tggcgatttg ggatattatt ttccgctttc atcatgccca aagactgctt tgttggcttt
    19501 atatcatcag caaaacgagt catagcatcg acctgctcaa tcgtcaaaca actgtcacct
    19561 gccgtggcat tgcagctgaa attagaattc attgggccac agccagttaa aagaactgag
    19621 ataatcggca ttaggacaac aatagagtgt ctcattgtaa acctcctgtt tcattgcggc
    19681 ttaaggtatt cacacggtta atttttccca agacttctgg agggactgta aaattcattt
    19741 ccgagttctt ctcttcctca cctctagaaa tcaattcctg tgtttcctcc tgcgctttat
    19801 acctctgcat cgcatgttgg ctcttgtctt catcgggctc caagtaaaag ccatctttaa
    19861 agacgatggt aaccacattg ccagaaccta cctgaatcac cggatggtat tgttctgctc
    19921 gtttgacata gtactgagat aaggtgtcag ctgctttgga agcaccacca taagctgcaa
    19981 agggtgcaat ttgactgctt ggcacaacgg atgttgcgcc atatggtcca atggcttgta
    20041 cgctttgcgc gtattgtgca gcacttgcaa taccagacaa ggcaccactc acaccagccc
    20101 aagtcatcac tttgttatct cgcatcaatg gtttcccttt aatacccacc ttgccattga
    20161 aaaacaccca gcctgtaact tccttgtcaa taatgggttc gcctggatga gcgcaagaca
    20221 aacgatagag tgtggcaaaa gcacgctcgc tggaaatatc accgtaggaa ttggcactga
    20281 ctcggcaccc tcttaagcgt gagcgctgac cattcggtag cgtaccgtct tctagaaact
    20341 taaagagcat cacgccatta tttttcgatt ggccattcac tgaagcatct gcatccgcac
    20401 cacctaaaat gacggcacgt actgatgtat tagaaggcac ataatgagca ggattaagat
    20461 agaaattgtg gtgcttcttt gtaatgtgtc gttttggacg aaaagagacg gtgtggatac
    20521 gtggaatggc atcagacaaa accgtacctc tattcatcgt attattcatg gaatacacgt
    20581 tggggttatg ccatagctga gggttttgaa cttcctttaa ttcggaaggg ttatcttgtt
    20641 gttccgaatt actttgtttt tcctgattag ctaaaaccaa ttcttgtact tgagaggtca
    20701 attcttcttt ttgcgcttgc aattcttttt gatgtgcctc ggccattgat ttaatgttaa
    20761 gggtcaattc actaacttgc ttttttaatg attcaagctc tgtttgttgt gctgtgagtg
    20821 cattgtcagc caccgcatca gtaaacgatt cctctatgat tcctgttaaa ttcactgatt
    20881 ttttagcctc aagattcttt ttgggacgat catcggccaa tagcacaatc atcatagcaa
    20941 agagaaaaaa cagaccacat cctacgacaa tcaaggtgcg cacttgacgc cctttaacca
    21001 ttttattcca ctgccagtta gccatgatga ctcgcttcca cacgatacag catggcacgc
    21061 tctccaggtt taatggaagg tttggaaaac ttaatcgcaa gcgtaccttc tttagcaaac
    21121 caatcttgcg ctaattccag gggttcttta ccgccattgt acaattcgat ggtttcgccc
    21181 tttaacaact tgctgtccca cgactctttg ggtaaaagcg ttaatccttt tgaccagtgc
    21241 tccgcgcggc caaattgacg tcttactttc acatcggcaa atggcttttg ttgttccatg
    21301 tgggttagca tggcaataat agcttcaggg actgcttcct gagtgatttt tttagggttt
    21361 gttattgggg cgtgagtctt ggatgcagca accactggca gcacgggaac cagttcaatg
    21421 gtcttgccca gtgactcttc tccatttacg gtcactgaaa aatgtcggcc tgactcagtg
    21481 gttaaaaata aagtaaaggg attggcggct gcgagcataa catacaccga gccatcctgc
    21541 tcatcacgct taatggccat agcgtttggt gggaatacag cttccataat tttatcgttc
    21601 ttgactacaa gacggttgta gttaccctga cttaaagtta atgcaatatc actgttgtct
    21661 ttcacgggga tgctgttagc tcccattgca accgttgata ataaggccag cgcaatactg
    21721 aatggtttat gcatcttgtt tctcctttaa aggcgcaaac tgaactatgg atagacggct
    21781 gtctttgtat tggaactgca aaacataagt tttacgctcg tcatttaatg cttgaagccc
    21841 tacaaaccgc ttcaaaatcc ctgaaacaac gacagtcagt tcgtgtgggt tggctttatt
    21901 tttatcaata taaaaaatgg acgacatttt tttatctttg atgaccttgg cctcattagt
    21961 tagtagccgt agcatttggt tgtagttttg atgcccaaca taagaaagaa ggcgttgatg
    22021 gctggcatct accgtttctg gtgtgacgct taaacgttca ttaacaaagt tttctgccat
    22081 caggcttaaa tagtggccat caacactgga agctgatttc aaataagaag gcgcaccact
    22141 aaaaggagtg acctcaatcg tatgttgatt ccatgcttta aataaaaagc aagacagcaa
    22201 aacgttcgat atcaaaaggc caaatactaa aacaaccatc agattaaacc gtgctgacag
    22261 ctgacttaag cggctttgat aacttttaaa atccatgctc tctccttaag ccacatacac
    22321 acggtggtgt gataaaggta atttgggcag aaaaaactga gtgatataac tgggtaaata
    22381 ccaataagcc aacactaaaa gcacctttgg accctcccct ttcttcaaat gccgcaaccc
    22441 acttaacaaa cccaatccca acattgatac caatactttt tgattggaga ggatgagcaa
    22501 gataaagctt aaggccgtca ctaccagctc atccatggtt agcgttaaga ccctcttggg
    22561 cgcatcaatg tggtttaaga attgatagtt catgttactc atctccttcc tccatcaaaa
    22621 aacaaaggtt ttcagtaaaa aggctggaat aaaggcaacc gcagtcacgc caataaacac
    22681 catgggattc ttgcttttga cggccattgc ggttgcaaga atgatgtcca ccaaaataaa
    22741 gattttccaa aacatcgaac cacttcccag cgaatctttg acatctcctt ctaatgcacg
    22801 agccaataag tcattggcaa agacactttt tgaaagcatt gcaaacatga acgccgagcc
    22861 tgctttttgc atcaaactga ctatcttttt acccattttc attactttct ccgtttttag
    22921 gttataaaaa tcccttaagc acctaaattg cttaattttt aagcaaaaat cacaatgttt
    22981 ttattacgag aataaaatct ctttttaagt tctttgactc attagaagat tcctgaaatc
    23041 actttgacta atccctgttc taatgaagga cgagcctctt gaaattttaa attgactttg
    23101 tctgcgttag ataccacgcg ggttctatag cgctgatatt gactatcgcg cgatgatgtt
    23161 tgggaagtaa ccgtgctgct accgtttttt aaatgagatt ggaactgctc attgatttta
    23221 atgcccttac cgactctctc actgatttgg acatcggtta tcatggtgta attcacatct
    23281 ttgactaaag aatcagtagc catcccaatg accccgccag caaggccacc cactatcata
    23341 ccagtgcttg attgagtcaa acttcctgca gcactacctg ctgctgcgcc cgcaatggca
    23401 gaaccataac ccccacccaa cgcagattga cttgcagcaa cgctcatttt acccaccttg
    23461 agtacgttgg cttgtaacag atagtgagct gaacttggac tattaacaac cttatagcca
    23521 tgcgcgctta agactgtttt taattgagga gcaatatcca cgtcttcatc cgatgtgttt
    23581 tttactgaaa cataaactgt cttctgagtg tttgggactg gatccaaaaa aatcgtttca
    23641 ctttgtttgg tgctgacttg taaagaaccg tgctctatag cagtatgagt tgctgcacat
    23701 cccgtaagca ctattacaac actagccagt agggttttag taagtcctga tttcatagtt
    23761 tcaatcattg tattatcctt atttgtccta acaattagtc atgaattcca gtaaaacact
    23821 tcctaaactt ttttattaat cgattaatca tagttccatc ccaactgatt tctcgtctat
    23881 aagctccatc ctgaattttt ttcatgaacc ctcttgaatt tcaaaacgat ggaatggaac
    23941 acattctggc gcatcaaacg ctaacttcaa ctgaccatcc agttgatgat ctagtaccat
    24001 acaaaacact ttgttttcta tcgctactgt ctcccctacc ctgcgttcta atatcaacat
    24061 attccccttc ctcttctatt gattatttag aaaaagtaat ttattgatag catcgtttaa
    24121 tgcactgtct cattctcaaa tggataaatt aaaaacaatt gctgccgctt taataaagaa
    24181 tatgcaaaca ctgctgcttg tgcgtgttta tcaaaatcaa agccaccgat ttctgtagct
    24241 gctttttgca agcacaaaat acaaccttct aaaatatcga acacctcttg ttctgcattc
    24301 ataatgaatt tctttgataa gcgataatgc cattgaatca tcatttaaga tcaggtgcat
    24361 tcacctaggt gcaaggataa taaaaaagtg gaaaaaattt ttagggtgtg gaatttaatt
    24421 gaaatagcta ttgtaatcgt aggtaatcgc aaagtattta gataatttta catagtcgta
    24481 aaagagttgc tctagattgg agtcatacaa acctctatcc tttaaccaat taacaaactc
    24541 atctcccttc aacagattac gctcctctct aaatagttct acctggtaga taagctcgca
    24601 ttcggttatt tcgtattctt gtttatctag cattgctatt atagattgat aatgaagcat
    24661 attagctttt gttttaggaa tggatagtaa tttaacaata ttttcagctc gctgaagatg
    24721 agccacttct cctatttgct ctagttcatg agaattcaac caaacaggcg ataaagtagc
    24781 ttcaattaat tttttttcaa aaagggtaaa atttcttttt ttcttaccca aggctaaagt
    24841 cattggaagg atttgctggt gatttaaata aaacaataag gattttgcat cctctttttt
    24901 cacgtccgca taattggact taattaaatg ataaagttcc tctgaaacct gttgggcaat
    24961 cctaccccat gttctctcag cataaaatat tgatcgaata gaatctaaaa tcgtttcctt
    25021 attagttata tttattcttt caagcgtcag acgaatattg atcaaaggaa tggtttgctc
    25081 attgttgtat ttagaataag caatggcaac ttcatcatca ccatcaattt cttcattctt
    25141 atacatctca taaatacgcc catacccttt cataccaaaa gcatccagct ctgctgctct
    25201 caaagccccc attgaagcag ctccgaatac ctctaaacca tagtctaggg cagttaatat
    25261 ttctttatgc caaacagaag gaacacaaga aaaatttcca tcaatgatga caattcgttt
    25321 atatccatca tttattgatt taagcacatc acccttttta attgatggta gatactttgc
    25381 atctggcaat agatgcatcg cttcttgatg ggataaagag gtttctaaaa aaacgactgt
    25441 ttcatacatg gcttatcaag tccatagaaa ttagtttaac cttcacaata caaatttctt
    25501 tgtcataata cgtgtacact aaaatatcct gattattttc tttaatcttt tcatagagta
    25561 cagaaagtgc atcctctatt gtccctacag aataatcctg tacttcatcg aagttctttt
    25621 tattcataac aacaggaaac tcactggcct tagaatcata tttagtatta attagatcat
    25681 ctcgagaacc tgctatagta gttactcgcg attgaatggc ttcagtgagt gcacgattta
    25741 aggctatttt tttatttaaa tggcttccac tacccttaaa taggatttga ttttcaaaag
    25801 gattttttga tttaagcaac acttcaaatg aaggaactct ataataattt tcttgataag
    25861 taatgaaaca atcaaacctt attgataaat tttcaactaa attgctatta acaaaagcaa
    25921 tctcggttat ctgttgcgcg tcttgcctct caattacttc caaaattccg tgtaacagag
    25981 cttctttata gttatttcct cctgcgagtc ctgttgtatc aggagaataa attaaaactt
    26041 ccggtaaata agaatttaat gaatattctg caaaaggtac aagaatggtc ttccctgaga
    26101 atactaaatc agcatatacc caatttattg gctgtgaggg attggtaaac cgcactgagt
    26161 ttgcaagttg atttattggt ataaaaaggt tattactttg tgttaattca agctctgatt
    26221 tattagtcac ttgtggaatt atttcttcag caaaatacac ctcaatcgac tccatcaagg
    26281 ccgaacattt agctgcttcc tttgttaatc cttttccttg actcgttgtt aatgattttg
    26341 ctctaggcct tattgcagta tagacgggta ggctggtgta atctaaatgt gttaagtctg
    26401 ctaatcgagt aattcccgct agtttcttaa actgattcaa taagtttagt gtttcactga
    26461 aatgacgtgc tctaaaagaa gtttctgcat gcctaataat catttagaca gactcgtttt
    26521 gaggtaataa gcatcaacgc ctaacgttct aagctctgaa gcaaacaatt tacttcgata
    26581 ccctgaagca caataaaata ctaatttttt atctttataa gataaaaaaa acgcataatt
    26641 attattatcc ttaataggaa aaaataaatc atcatctaac tttataatct ctctctcctc
    26701 tttttgcctt atatcaacca gaaaatgctt gtttctatca agatcttcta tggagatccc
    26761 ataatcagca ctactggttt gcaaataatc aaagttgatt ttcttttgtt tgcatgcaat
    26821 gcattcatta ttttgcttta tagtcaaaga ggagatacta aagttttttg catctactat
    26881 tcgtacttgt ggtgcttccc catcctctac cttaagtaaa taatttagag caaggttagc
    26941 ggccatggtt ccggctattc ctgttactgt gcctaaaaca cccgcttcgg aacagttagg
    27001 aattgcacct gtaggaggcg ggttaggata taaacaacga taacagcaat gtttcgtatc
    27061 aaataaaaca agctgaatga catttacaaa aatactacag cttataaata ctttttcttg
    27121 taagacacaa atatcgttaa taaggtatct cgtttcaaaa ttgtctgttc catcgatgat
    27181 cagatcgaca tctgagactg atgagtaacc gaaatctaca tcaaatttat tggtgtatgc
    27241 ttcaagaact atattgcaat taacttgact tagagcaact ttcgctttct cagctttata
    27301 atctccgaca tctgcctcat taaataaaat ctgtctatgt aaattagaaa gatctacttt
    27361 atcattatcg actaggatta actttccaac accagcagcg gcaagatatt gagcaacagg
    27421 acatccgata ccgcctagac caacgatcat aattcttgca ttacttagta ttttttgact
    27481 ttgctctccg atcactgcga tctggcgttt ataccgttcc attcctctac ccccctgata
    27541 tcgaagttac aatacagatt tcatcattag ggttaagtgc aatttcttta actgatgtaa
    27601 tttgttttga gttaacaaat acagaaatgt atccctttga acaaccgtct tcatcaaaca
    27661 tcttttcaaa cttctcttta taaaattcta ataatacttg ttgcaagttt gattgattag
    27721 aaaccaattg gatttctttt ttggacgaac taaactctat taattggtat tcatgcattc
    27781 ttataacctg tagattttta tataaacgat atatataatt tatttatatt tttaacaatt
    27841 gtggtgaaat atgaacgaac aactgtttaa agatcttact aaaaactcat cagcagccaa
    27901 aatgaggcta taatctattt ttaattcaat aaacctctac aatatcttgc tctgcaagaa
    27961 agttaccagt cttatttatt tcagttacag agtcatccca acaaagaacc atcgctgact
    28021 tagacatatg tgccatttgt tcgcttgtat catcaggctt aacaaaaaaa gtccattcat
    28081 tttgaacatg aagttcaggt ttgtttatct tttttataac ccccatcttt gtaggaaagt
    28141 aaccacatac gaagtgactt ttattttcta ccatgctagg aggttcaact ccacaaacaa
    28201 tacaacataa aattgtttca aggcttatgg aatatttcct ctggtacaac ttatttaaac
    28261 caatacctgg tggtctagca ttggtttcga taaattgaat ataaccatcc tcattcatga
    28321 agaattcagt atgaagtacc ccattatgga atcccaataa ctggctcacc ttaactgaag
    28381 catcaataat tgattgtcgt tgaggcgctt ctaaaatatt caaactaaat ataggaaaat
    28441 ttttactaac cattaaatgg tttggatggg tatatttcct ggcttctaca aacaaaatgt
    28501 tcccatcaaa aactaccaac tccgaatgat ataaatcatc tgccacataa gcttgagcga
    28561 tatagttttc ctcatcctct ttcttattgg ctaaaaaatt agaaaactct ctctcagtct
    28621 taatatgata ggtctcatag gaaccagagg aattaatagg tttaacaaaa atctcttgaa
    28681 cacctatctt ttcttgaatt gattttaaat ttggagattc aatcaatgtg gttgttttag
    28741 gaaatggaaa tgaaccatcc aacttttgat acataagatc cttgtggctt aatagcattg
    28801 ccgtattcga actcatacca gcaatcttta gatgttctcg gatttttgct atatccacga
    28861 acatatcctc agaaaaacac acgatatact ttggacgaat tgaatcacaa attgctagga
    28921 tatcttcata tgaaaatgag tttactaata cattttcccg atccttaaca gccttagatg
    28981 catctttaat caaaaaaaag ggagtaatgc ccaattgaag aagcatctca caatcgatta
    29041 attcatagtc ctgaagagga tcaatgcata acagtgtcgt tattctcatt atatttaata
    29101 ccaaaacatc ctactaaaaa cgttgttttc gaagggaatt gatcaactga atacccaagt
    29161 ttatttgtta cttccaaaaa tcgaggatcg taagaaaaaa tcgtctccct aggtagttta
    29221 aataagttat tagtcacttg attgttcttt tcatgaaaaa gtgttaaatc gagcattgtt
    29281 gcactttcaa aaaatggcgt attcttaaga tggcctaatt tctttatatg atcagcgtac
    29341 tgaaaataag tatcgtatat taattcacaa cgctgtggtt tatttgaaaa tgaggtaggc
    29401 ttaaaagcca tctcttcttt gaggatcgaa taaaaaacag ggttgttttt atttttgtca
    29461 aaatgaacag gctccataac aaaaggagtg tatttggatt tatatatatc gcgatacgtc
    29521 tcaacaataa aatctaaggg caataaatca tcccctacgg ctaaaccgta accgggaact
    29581 tgtcccgtac ctatagtaat caattgacta atggcgttat tagtaaattc attttgagat
    29641 atttcaggat tattggctcg gacgtaggcc attagataat tataaaaagg taagtttgca
    29701 taataacctg tctgattaga atcaaattcc atgactaaac tcaaaagagt tggcaattta
    29761 ttctctttaa ttaacgttac aatttcagaa ggaaacacca ataattttga acgccactta
    29821 cagttggctg cctcacataa actcttaaag gttaaataat gatttccaag ctcatctggc
    29881 acaggaatgt cagccttaaa atatttttct aaactggcat gtggccttgc gtctgccaca
    29941 ggacaaagta aaaaggtatt tcttgcacct gatgatattt gcagtatatc tgtgggatga
    30001 tgagtatttg aagcattata aagtgaacta acgcttaaaa aatccccttc tttatagact
    30061 tggtgtgtgc ttgacttccc tttaaattca ataaacattt caagattttt ttctaaaacc
    30121 attgaaaatg gaaccgagga tagctttcca caagtatttg gcaaataaaa atagtcctca
    30181 tccgcaatga tttgtccata ctgatattca aggacagtaa aaagcatatt ttgaatgcca
    30241 tctatggttt ctaatatttc atagaggcgt ttattaacac gagctatgtc cgtttttacc
    30301 ctatcccaag ataattcact catatgacat caatctaaaa tcctattaat taaccgaatc
    30361 atacaagaga acattagtgt attcaatgaa cctgtatttc atgcaataaa atttattgct
    30421 aaaaataata atatttcttg cttaaatgca tttcaagtta taaattatat cacttgggat
    30481 gcatttaaat aaaaatggag cttttaatgg taattgacca tgttgatagc cagataataa
    30541 aaatgataat aaacggtagc caagttaccg aaatcgctga agataccaaa aaatcaaaaa
    30601 gatacatcct ctatagactt agtgacttaa agactagctt taattgcaaa accacgcctc
    30661 agctaatcta tatgttggcc acgtcagggt taattaagta atttcaccac atggatgttt
    30721 tatgaataat tcaatttact atcatagatt taacaagctt ctttcattaa ctaactgccc
    30781 taaagaagct ctgcttttag ataaaatcat atttcattat caaggcactc ttttaaaacg
    30841 cgaaaataaa ttatggttta caaaaaaaat tcctgatctt gctgcggaac tcggttttag
    30901 tgaaagcagg atttatatct acctaaaaaa tttagaagaa gaaggactga ttatccgaaa
    30961 aagatttaaa tattacggtg tacctcgatc ttttatagca attacagaaa cacttcaaag
    31021 caaattacaa tttctctcaa aagagaaaga aaacactata gaaataaaag aaaaaaaacc
    31081 tacatctaga aatgatataa acgagagaat ggattatctt gtttcagcag at
//
LOCUS       DATNSN010000021        31110 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.021, whole genome shotgun sequence.
ACCESSION   DATNSN010000021 DATNSN010000000
VERSION     DATNSN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 31110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31110
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.021"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            83..217
                     /gene="rpmH"
                     /locus_tag="VAH76_000771"
     CDS             83..217
                     /gene="rpmH"
                     /locus_tag="VAH76_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO1385419.1"
                     /translation="MKRTFQPSNLKRKRDHGFRLRMSTRAGRLVIKRRRAKGRKRLSA
                     "
     gene            218..562
                     /gene="rnpA"
                     /locus_tag="VAH76_000772"
     CDS             218..562
                     /gene="rnpA"
                     /locus_tag="VAH76_000772"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO1385420.1"
                     /translation="MFAFKKAQRLLKKNDFDFVFESAKKITTDDFIFLFRENKLGYAR
                     LGLALSKKMIAKAHDRNRIKRLLRESFRHTNLPAVDIIILARPGLAKKTNLGINTKLN
                     KTWEKLASCYGK"
     gene            529..774
                     /gene="yidD"
                     /locus_tag="VAH76_000773"
     CDS             529..774
                     /gene="yidD"
                     /locus_tag="VAH76_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO1385421.1"
                     /translation="MGKISLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSA
                     IKHYGVIKGLLMALSRLSRCHPWSKGGYDPLFPNDKN"
     gene            784..2454
                     /gene="yidC"
                     /locus_tag="VAH76_000774"
     CDS             784..2454
                     /gene="yidC"
                     /locus_tag="VAH76_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO1385422.1"
                     /translation="MDIRRIVLYMALALIGLSLWNAWQIDYPAAQPVEDKTASQLTSD
                     GHLLPQIIPSNAEQPVTLKAEEKASSGKQLIQVKTDVLDVGIDLKNGDIVKGLLLDYP
                     LSVEDKNKPFPLLQNQASQRYVANSSLFVLDGQTPQSLDFDFTSEKEYYELKPDQNQL
                     IVTLNGKSEDGLDVKKEFVFTKGSYLIEVNYKIANTGNSLWKGYFNTQLLRSSPKEDK
                     SSIFHIGSYTGASFSNPGKNRYQKVSFSDMSKSNLDVDAKGGWIAMQQHYFLSAWVPN
                     ADSENKFYTLATDKDYTIGAVSQPITVKPKEDKIVGSKLYIGPEITSVLKGISPSLDL
                     TVDYGILWFLSSLLFSLMKAIYTVVGNWGWSIVLVTVLIKLAFYRLSATSYKSMASMR
                     KLQPKLQALRERYGDDKAKISQATMELYKQEKVNPLGGCLPILIQIPVFIALYWVLLE
                     SVELRQAPFIFWINDLASADPYHVLPLIMGATMLIQQKLNPAPADPMQAKVMMFLPIL
                     FTGLFWNFPSGLVLYWIVNNTLSILQQWYITRKYSDEKPAKKVVATAK"
     gene            2455..3795
                     /gene="mnmE"
                     /locus_tag="VAH76_000775"
     CDS             2455..3795
                     /gene="mnmE"
                     /locus_tag="VAH76_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO1385423.1"
                     /translation="MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPR
                     LATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGA
                     RLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
                     ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSL
                     VIAGRPNAGKSTLINNLAGRDVAIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDS
                     DDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTLPNKIPIITVYNKI
                     DTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAK
                     ALLLTGQSQLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK"
     gene            4090..5928
                     /locus_tag="VAH76_000776"
     CDS             4090..5928
                     /locus_tag="VAH76_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg3000 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385424.1"
                     /translation="MVYSKQSAAFRRDKQQFFAQIPKDSKLDTKIVTIDDRVNTIDYT
                     FLLHHKQDLEAEFTQMFTVLQQQDDENKEIFWMYCYYCASLLEAFHKAYSQNSKQAEY
                     AKIKQKIKDHLNKVNVQDEEPGFIQSLYDSFLGGFRNLISSPLHASKIRDYVAYSNLC
                     RIYWTFCRLTLTNGLSVARDLKIIEKLDAILGTHTDVDKIISTLQAPTGVITYFSVGF
                     FLMRLIIDGGMLLRHTFFPTELEKGAENGCEIHQMDYLPGPASLEAYRKSYILVHEDN
                     QEVSLYYIPRSGKAEKLSIKENNKLKDALLKKMDKDTSSLRLNSTEVEEIITHQTGHV
                     PEVTTAWDRFKHEFYKRHCNFANDFVWATVNLITNYNYLFNISGPAAGMITSVFLGFD
                     VCMALYKCNLAKQEYLIKKAQYLQEIEDYNNPEKFKKMTPEQRLLHIEMLNKQLRELE
                     INYKTQEATFYFVAAAAALLMMGFTASMLVSPPVLVVGCFFACTLAVAMYLSTGAYSK
                     YEEKSLYLEEAQLTGKNLPVALKEYEAARNDFIFTMVKNTVMPIVLITTFAICWPAAI
                     VLTAMYIGYEIFHAYDQHSEAKAAKQLALAAPSEEKDGYMALATPN"
     gene            complement(5925..6725)
                     /gene="legP"
                     /locus_tag="VAH76_000777"
     CDS             complement(5925..6725)
                     /gene="legP"
                     /locus_tag="VAH76_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegP"
                     /protein_id="HEO1385425.1"
                     /translation="MKIKSWILSLFYSSLCFSSSYAINLNQITITDPLMGSRTITYEQ
                     VGEFAVVEGDILIGKAENLKNQGAVITPKVGGSRWPNGVIPYEIAENLPFINKLAILQ
                     AIDHWQKNTNLEFVELTSKNRYDYRDYISFIPAEGTTCSSYVGRQGGKQEINLAPRCT
                     TMNTVHEIGHALGMWHEQSRTDRNQYIRIVWENIDEEHKFNFDQHLTDGKDFGEYDYG
                     SIMHYGRFAFSKNGQPTIIPLVDGVEIGQRTHLSEKDIAAIKAMYPET"
     gene            complement(6928..8676)
                     /locus_tag="VAH76_000778"
     CDS             complement(6928..8676)
                     /locus_tag="VAH76_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385426.1"
                     /translation="MTIECYSIDGDECILRKEYDDAPDSEKFSGKAVIYTNLPLIKKI
                     KHEIAKNKLHPIIVFNGSSRQSMFMEQLNALREKKLFRGSFCLALQKLCDYLKVELDK
                     TLLPDITGDLEYGTTFNRIMDEVLNIEWLDNKKLQHKHVPYPDYNENWHAKLDPFKRI
                     LLFAQIQKIRNDHPDEDIIFNFIDDKDDILSTLKKYFINYSYMIPHGVTLRLNHYDGS
                     NVSSYASIKGTGRPYKNYRQIVKDMHEHVYNLDAFAETINQNDKFSEIPAVRQILEHE
                     FFVSDPDDVVSCLDKGKIPFVIRKSKSQISGYIMFTAAYKTSQGIFANRYGINTEGEL
                     YKFFDSQIEKMDIDSEGIIAALEREIVITKMIIEKTENLSKIPWKSQPEKKNNDLDYQ
                     ENGEKEAETNPSELFTPMTKKTKSPQRENKQKSLCSQLRSCKFFVSSVNNAESELNKK
                     QLPFLIRPSRATSNEYYMFTTVCKTSKGIFNHRYGIDRKGNLYSFDKDGTQKVNIMPK
                     GIIATLKKEIAEETEKIEKVKDIAQITWVPDEYKSSKSSIKGKNRKPEWHLKFFNENG
                     DSAQVSNTTPTLCKNI"
     gene            9240..11405
                     /locus_tag="VAH76_000779"
     CDS             9240..11405
                     /locus_tag="VAH76_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC26A/SulP transporter family protein"
                     /protein_id="HEO1385427.1"
                     /translation="MTRSQLILAILAGLVISIDNMISIVAFSSLIYQGSLEIYIPLVI
                     NILAISVIIISASYLFMSSPPYAIAQLQDEAAILYSAMALVIIKSMPSSSTNEAIFIT
                     ILFMLGVTTCLTGLSFYFVGKFRFGNIIRYLPYPVICGFMAATGWLVLSGTFALLTGK
                     ALHQNWLIFFHKEEILLWAPGFIAGIVVYIFKEKLGYRYALQLMFISLVILFYALFYI
                     FNLEYLVDPTEGYVLGPFNQSHIDSLMKPEIWQMIHYPFSMTLLNYIGLVILVSFIAL
                     LLNASSYELIVNKQLDLNKELRIAGIGNVFSGLLGGMVGYLSLSASTVAKEYGGARII
                     GVMAFVPMLLILCFGAVVVECFPKMAIGCYLFYIAFCFLSEWLINTWRLLNLSEYLIV
                     LLILVIAIVFNMITAVAIGTVISIFIFAFRYSKINPVKYLFSGSDYNSSFERNPISQS
                     ILSTQGDEIQFLKLQGFLFFGSIYRLLQLIKSIQKARYIILDFELVYNIDSSRIILMK
                     NLKLHAEKNNISFAICSLKPIVYHELMKTNLLQSKTNWLKVFTDQESALEWCEDQVIE
                     KYTENINIEEVTLEKQLLYLGFSSELVAWISEKASIKPYNSDDIICHEGEESNSVYFI
                     HRGKVTAFVGDKRVLVVGSGNVLGEIAMYTHKKRTATLKTNEKTIVYEIDLKSIQELS
                     KNKPLLLVQFHMCMAKILANRLSVLNKRIKVFDSTAILQ"
     gene            complement(11456..12523)
                     /locus_tag="VAH76_000780"
     CDS             complement(11456..12523)
                     /locus_tag="VAH76_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEO1385428.1"
                     /translation="MDDLKKYSFLLVYLLIPLPFVSLYLGGWYSFLPFLIVFAFIPLI
                     DAFLHDSVNPTKEQEQELLKDKFFKYITCAYVPIQITIIAYGVYIVSTQNLVWNEWLG
                     FTMSLGLISGGVGINFAHEMMHKNSKLQQLMSKILLVTVCYGHFFIEHVKGHHVKVAT
                     PDDPATARLGENLYQFLPKTLLGSFQSALLLEEKRLSHKNYSIWSIHNNFWWILTVPF
                     IICIVCFIFGGLIALSFFLLQSFIAILTLEIVNYIEHYGLERKMLSNGQYEKVNPNHS
                     WNANHWLSNQLLIHLQRHSDHHAHGARPYQILRHMENSPQLPSGYLGMIIIALIPPLW
                     HYIMDKRVVAYRIQLRQLEQL"
     gene            complement(12653..13504)
                     /gene="rsmI"
                     /locus_tag="VAH76_000781"
     CDS             complement(12653..13504)
                     /gene="rsmI"
                     /locus_tag="VAH76_000781"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO1385429.1"
                     /translation="MTNTLATGLGTLYIVATPIGNREDISFRALNTLKSVDLILAEDT
                     RHSMQLLTSLGIKNNLTSLHAHNEANKSNEIIELLLDGKSIALISDAGTPLISDPGFP
                     LVKQARQHHIPVVPVPGACALIAALSAAGVPCDSFAFLGFLPAKQSARKHALESSRSV
                     PYTIVFYESTHRIIDCLNDIAEIYGQDYELVLAKEITKTFERFVSGKIKEIKDWLLSE
                     PGHIKGEFVLIFPPRSTNKDLNSHEELLKILLEELPLKQAVAIACRLTNANKNQLYEE
                     ALKLKGS"
     gene            13544..15355
                     /locus_tag="VAH76_000782"
     CDS             13544..15355
                     /locus_tag="VAH76_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEO1385430.1"
                     /translation="MLIKQLKLRMFLVLVSLFLLCQCTKTVNSTEPVVTVNKKLKNPY
                     SLPTAAYLAMAKSQQGHEKQSALISAAGRLITDGQWKQGTAILAQTGELTVEQADEKN
                     LLLAKVDFMRDRPRDALAKLARIKEPERLSLYNQIQFHEQLAKSFRSIGSYSESVSER
                     IKLESLLPDEESQVNNRRALWLTLTSLPQEELNTMALEAVNKSEMQGWLQLAVISRKY
                     RNNSKSLLAALDQWQMHFSNHPANQILPNPLDSISDKLLAPPKQVALLLPLSGPLSGP
                     GNAIREGFMAAYKANRGDESTKIKVYDTSKGDITNTYRQAISDGAEYVVGPLTKTQVA
                     TIASMEHPVPTLLLNDTDASTQNNSYSLGLSPVNEAIQVAIKARSKGYKKALIIAPNN
                     AWGNEVTKAFINQWNEDGGHIVDSLRYEAKQDLNKSMKDFLQITNSQEREKKLKQVLG
                     YNFQSTTSRRQDFDMIFLLAYPSKARQIMPLLKYYYAGDAPVYATSSVYGGSANALKD
                     KDLDGIIFCDMPWVFSHQMGTKNWPEQFNSYNRLYALGVDSYTLATQLNQLILFPADG
                     SSDSTGILYLKPTQQVARVLEWGQFKQGLAHSLGDTV"
     gene            15377..15733
                     /locus_tag="VAH76_000783"
     CDS             15377..15733
                     /locus_tag="VAH76_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEO1385431.1"
                     /translation="MTQEKGKFAEQLALNYLKENGLALVMQNYHCRLGEIDLIMREGS
                     YLVFIEVRSRSNMNFGGGLASITYEKKQKIIKATSHYMIKYRIQDKFPIRFDVISIDG
                     KSNKITWLKNAFDAGC"
     gene            15763..16359
                     /locus_tag="VAH76_000784"
     CDS             15763..16359
                     /locus_tag="VAH76_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEO1385432.1"
                     /translation="MQLEERVRHLFGVTIEAKISAADMLSGSIAKAGKLLVNCLLNDG
                     KIFICASGGSSANCMHFSSSMLNHFEVERPSLPVINLCADASCLSSFADEHHFGQVFA
                     RQIQALGQEQDVLLLLTTSGNSDSMLQALHAANERGMDTIALSGRDGGVLANHMGPED
                     IEIRVASDSTALIRELHLFILHCFCDLIEQSLFGQVLG"
     gene            16363..16935
                     /locus_tag="VAH76_000785"
     CDS             16363..16935
                     /locus_tag="VAH76_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEO1385433.1"
                     /translation="MKMNLKFKSIIVMMIGALLTGCVAVVVAGAAAGMVYDRRSVTTM
                     EADARLFHVIHKTIIKDPRFRHSRILVSSFNRVVLLVGQTPAASLRVLAERIAQNAPG
                     VKRVYDEITVDNPIPLTQRTKDSWITSQVRSNMLTKKGLESGSIRIVTENGVVYLMGI
                     VSDEQALLAVDVARRVAGVRKVVKIFQYIR"
     gene            16937..17524
                     /locus_tag="VAH76_000786"
     CDS             16937..17524
                     /locus_tag="VAH76_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEO1385434.1"
                     /translation="MFKHGCFILIVLFLASCLSGCLGALWTGATLAYDRHNVYKKLDD
                     YNLIKALNDVLAVNRTFKNSETVLDIAVFNRDILIAGHVPTQELYDELQLRLSKVKGY
                     RRLFNRVIINKMPSNSIQDSWITTKIRSQIFADSSIDPNAFKVVTSDRVVYLMGDVQT
                     EQAEKVIEIARYTSGVQKVIKLMRYYTYQTSTNMA"
     gene            complement(17594..17839)
                     /locus_tag="VAH76_000787"
     CDS             complement(17594..17839)
                     /locus_tag="VAH76_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385435.1"
                     /translation="MSEKIQHTAARLQEKMSNRYSDGLSHKHNKNHLSYRQHCRSHPC
                     NQTNNKDEENIVNGSTGLLREFNLRKIRKRLDKGRFH"
     gene            17993..18388
                     /locus_tag="VAH76_000788"
     CDS             17993..18388
                     /locus_tag="VAH76_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEO1385436.1"
                     /translation="MNKQLNKRGITRLWLAQLGVTVILAGLCAILFGVNAASSALLGG
                     LVCIIPNAYFAIKLFKHQGARAARQIVNSFYKGEALKIILSMFLFALVFLLCRITPAA
                     FFASYILVLMTHWFAPWIIVNKQNRPKSD"
     gene            18390..19217
                     /gene="atpB"
                     /locus_tag="VAH76_000789"
     CDS             18390..19217
                     /gene="atpB"
                     /locus_tag="VAH76_000789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO1385437.1"
                     /translation="MVSSTNYIKHHLTYLTYNVNDMTFGSSGGFWTLNLDTIFFSIAL
                     GLIALAIMYLAARKVTTGVPGKLQNFAELMLEFADNQVKDCFHGKNNLIGPLALTIFM
                     WVFLMNFMDIVPVDILPVLAQLVGIHYLKVVPTNDLNLTFGLSLSVFLLIVFYSLKIK
                     GVKGFVKELTLQPFNHPLFIPFNLLLELVGLIAKPISLALRLFGNLYAGELIFILIAL
                     LTLNATTSSLISTMTLGTAQFILALAWSIFHILVITLQAFIFMVLTIVYLSLAHEDH"
     gene            19294..19533
                     /gene="atpE"
                     /locus_tag="VAH76_000790"
     CDS             19294..19533
                     /gene="atpE"
                     /locus_tag="VAH76_000790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO1385438.1"
                     /translation="MTVIAVALLIGLGALGTAIGFGLLGGKFLEGSARQPEMVPMLQV
                     KMFIVAGLLDAVTMIGVGIALFFTFANPFLSSLGS"
     gene            19585..20055
                     /locus_tag="VAH76_000791"
     CDS             19585..20055
                     /locus_tag="VAH76_000791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO1385439.1"
                     /translation="MDINLTLIVQMLVFAAFVLFTMKLVWPPLAKALEERQDKIADGL
                     AAAERGRKELELAQHRVKDELKQAKAHSADIIDKANKRASEIIEAAKEAAKREAQIQA
                     KLAQEQIAQQVNHAKEELRKQVAKLAITGAEKILMREVDAKANSELLDNLIEEI"
     gene            20057..20599
                     /locus_tag="VAH76_000792"
     CDS             20057..20599
                     /locus_tag="VAH76_000792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO1385440.1"
                     /translation="MSDSTTIARPYAKAIFEHALAEKKLSEWSEYLTLLAQVVLTPQA
                     TQFIANPASTDEQQIELLIEICGSKFKKNDALNNLIKLLTTNKRLMLLPEIKALYEVY
                     RAEQEKILEVDVVSYSELTPAQQQRLSESLSQRLSRKVSLKISIDPSLLGGALIRAGD
                     LVIDGSVRGKLNMLGTSLAA"
     gene            20617..22170
                     /locus_tag="VAH76_000793"
     CDS             20617..22170
                     /locus_tag="VAH76_000793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO1385441.1"
                     /translation="MSEQVALNPSEISELIRKKIDQFSVVSEARNEGTIVSLKDGIVR
                     LHGLADVMAGEMIEFPGGVYGLALNLERDSVGAVILGDSSTLAEGQKGKCTGRILEVP
                     VGKGLLGRVVDALGNPIDGKGPIESSGMSPIEKVAPGVITRKSVDQPVQTGLKAIDAM
                     IPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCVYVAIGQKASSVASIVRKLEE
                     HGALEHTIVVVAGASDSAALQYIAPYSGCTMGEYFMERGEDALIVYDDLTKQAWAYRQ
                     ISLLLRRPPGREAYPGDIFYLHSRLLERAARINADEVEKLTNGEVKGKTGSLTALPII
                     ETQAGDVSAFVPTNVISITDGQIFLDVDLFNSGVRPAINSGLSVSRVGGAAQTKIMKK
                     LGGGTRLALAQFRELEAFSQFASDLDDATRKQLERGQRITELMKQKQYSPLTVAEMGV
                     SLFVVEKGYLDDVPVNEISSFEASLHDYMRSTHAALLHAINEAGAYDNEIEAKLKKAV
                     EEFKNTGSW"
     gene            22260..23126
                     /gene="atpG"
                     /locus_tag="VAH76_000794"
     CDS             22260..23126
                     /gene="atpG"
                     /locus_tag="VAH76_000794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO1385442.1"
                     /translation="MAGAKEIRSKISSINKTRKITRAMEMVAASKMRKTQERMRASKP
                     YANKIYEVIKHIARAASEYRHPFMSEREIKRIGIIVVTTDRGLCGGLNSNLFRETIRT
                     IRNWQEHGKEVDIAVIGRKGQAFFRRVGGNILGSIDHLGDTPSINDFIGVVKIMLDAY
                     YNGAIDSLHIVYNEFINTMTQKPFVKQLLPLPKSEEDKKTLGHHWDYIYEPEAKELLD
                     EILERYIELQVYQAVVENIACEQAAKMIAMKSATDNAGDLIKEFQLAYNKARQAAITQ
                     ELAEIVGGAAAL"
     gene            23139..24515
                     /gene="atpD"
                     /locus_tag="VAH76_000795"
     CDS             23139..24515
                     /gene="atpD"
                     /locus_tag="VAH76_000795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO1385443.1"
                     /translation="MSLGTVVEVIGAVVDVEFPRDSVPKVNDALKLVDSDLVFEVQQQ
                     LGDGVVRTIAMGTTDGLKRGLKAENTGHPIQVPVGKKTLGRIMDVLGRPVDDAGPIDA
                     EETWAIHRKAPSYEEQAGSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGM
                     LQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIVGQEHYDTARRVQQTLQRYKELKDIIAILGMDELSEEDKRVVTRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIKGFQGILAGEYDDLPEQAFYMVGSIEE
                     AVAKAKTL"
     gene            24527..24949
                     /locus_tag="VAH76_000796"
     CDS             24527..24949
                     /locus_tag="VAH76_000796"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO1385444.1"
                     /translation="MSITTHLDIVSAEHEIFSGVVEMVVATGELGEIGITPGHAPLLT
                     VLRPGEVRITLQGGTQDIYYVQGGMLEVQPHCVTILADVAERAEHLDEAAALAAKAKA
                     EAAIASKGGDIDYSVAAAELARAVAQIRAIQKTRKKMK"
     gene            25567..27822
                     /gene="parC"
                     /locus_tag="VAH76_000797"
     CDS             25567..27822
                     /gene="parC"
                     /locus_tag="VAH76_000797"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO1385445.1"
                     /translation="MKKIPNMNEVTEKKPLTEFTEKAYLDYSMYVILDRALPSIADGL
                     KPVQRRIVYAMSELGLKATAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYR
                     YPFVDGQGNWGSPDDPKSFAAMRYTEARLSHYADVLLGELLQGTVDWVDNFDGTLQEP
                     ALLPARLPNILLNGATGIAVGMASDILPHNLTEVANACIHLLDNPQADIKTICEYIKG
                     PDFPTEAEIISPSETIRAMYESGNGSIKMRAIYTQEKQNIVITALPYQVSGARVMEQI
                     ALQMQQKKLPMVEDLRDESDHEHPTRLVIIPRSNRVDIESLMSHLFATTDLERSYRVN
                     FNMIGLDGKPRVKGLLAILTEWLEFRLTTVKRRLQFRLEKVLERIHILEGLLIAYLNI
                     DEVIAIIREHEQPKQGLISRFNLTERQAEAILEMKLRHLAKLEEIKLRGELDELSDER
                     DTLQKTLASHSLLKELVKEEITKDRDEFGDLRRSPIITREDSQALKEEDILPNEPITV
                     VLSKNGWVRAAKGHDVDGKELSYKAGDEFKAQTLARTNQQVLFFDGEGKVYSLPGHAL
                     PSARGQGEPLTGKLNPAEGVLFEAIVGGEPEQKVLLATDSGYGFIAKIEDLYVKNRNG
                     KACIKLSENSHILPPRLIPNREQLFVACATNVGRLLIFSLEELPELSRGKGNKLISIP
                     VAKAVSREEYIVDMQVLTTNDSLTVHAGKRHFTLKGEDLTHYKGERGRRGNRLPRGLQ
                     NVSQLHVTQSE"
     gene            complement(27929..28783)
                     /locus_tag="VAH76_000798"
     CDS             complement(27929..28783)
                     /locus_tag="VAH76_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEO1385446.1"
                     /translation="MNFNIIGAGRLGKNLGIAMSVHQIASLNSICNSNLASARIACNE
                     IGFGNAVGKISDLSEADITWITCRDDSILDVVSILDNIEILKKGSLVIHCSGALNSSV
                     LLPLKKQGCYIASAHPLKAFRTNSLDSNAFKQVHCVIEGDYEACRWLRLAFEKLEASI
                     IAIQPEMKVIYHTAATIASNYLVTLASYSEELLLKAGIQQAQSRKMICDLMYSNILNL
                     NQVDRAKSALTGPLTRGDMQTISLHLEAIDNSEIKRLYKSMALATLPLLDLSLDRKEI
                     LRKTLEKE"
     gene            29035..30695
                     /locus_tag="VAH76_000799"
                     /pseudo
     CDS             29035..30695
                     /locus_tag="VAH76_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947875.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
BASE COUNT         9700 a         5309 c         6389 g         9712 t
ORIGIN      
        1 aaaataatca gttattcaaa ttgacagttg aaaaaaagtt atctatcatt gccaacctca
       61 taaatttaga agcagggtta tcatgaaacg cacttttcaa cctagtaatt taaaacgtaa
      121 acgcgatcat ggttttcgtt taagaatgtc tactcgagct ggtagattag ttataaaaag
      181 acgtcgtgct aaaggtcgta agcgtttatc tgcctaagtg tttgcattta aaaaagcgca
      241 acggctatta aaaaaaaatg attttgattt cgtgtttgaa agcgcgaaaa aaataactac
      301 agatgacttt attttccttt tcagagaaaa taagttaggc tatgcacgcc ttggtttagc
      361 attgtcaaaa aaaatgatag ctaaggctca tgacagaaat cgtattaaac ggctacttag
      421 agaaagtttt cgtcatacta acttgcctgc tgtagatata atcattttgg caagaccagg
      481 tctagcaaag aaaaccaatt taggtataaa tactaaatta aataaaacat gggaaaaatt
      541 agcctcatgc tacggcaaat agtgtgctta ccaataaaaa tgtatcagta ttttattagc
      601 cctttgataa caccatgttg tcgctattat ccaagttgtt cagagtatgc tgacagtgcg
      661 atcaaacatt atggtgtaat taaaggttta ttgatggcac ttagcagatt gtctcgttgc
      721 catccatggt caaaaggtgg ctatgatcct ttatttccta acgataagaa ttgagaatat
      781 ttaatggata tacgacgtat agttttatat atggcgcttg cgcttattgg tttatcactt
      841 tggaacgctt ggcaaattga ttatccagcc gcacaaccag ttgaagataa gacagcaagc
      901 caattaacat cagatgggca tttgttacca caaataatac cttctaatgc agagcaaccc
      961 gtaactttga aagcagagga aaaagcaagc agcggtaaac aattgattca ggttaaaaca
     1021 gatgttttgg atgtcggcat tgatttaaaa aatggagata ttgtcaaagg gttattgctt
     1081 gattatccac taagtgttga agataaaaat aaaccattcc ctctcttgca aaatcaggcc
     1141 agccagagat atgtagcgaa cagtagcctt tttgttttag atggccagac accacaatca
     1201 ttggattttg attttacatc agaaaaagaa tattacgaat tgaaacctga tcaaaaccag
     1261 ctgatagtca ctctaaatgg caaaagcgaa gacggtttgg atgtgaaaaa agaatttgtt
     1321 tttacaaagg gaagttattt aatcgaagtc aattacaaga tagccaatac tggtaattcg
     1381 ttatggaaag ggtattttaa tacccagttg ttgcgcagtt ctcccaaaga agacaaatcg
     1441 agcatttttc atattggctc gtatacaggc gcttcctttt caaatcctgg aaaaaatcgt
     1501 taccaaaaag ttagcttcag tgacatgagc aaatctaacc tggatgtgga tgcaaaaggt
     1561 ggttggattg ctatgcaaca acactatttt ttgagtgctt gggttcccaa tgcggatagt
     1621 gaaaacaaat tttatacgtt agctacggat aaagattata ctattggagc agtaagccag
     1681 cctattacgg tcaaaccaaa agaagataaa attgtaggat ccaaattata cataggccca
     1741 gaaattacca gtgttttaaa aggcatatcc ccatctctgg atttaacggt ggactatggc
     1801 attttgtggt tcttgtcttc gttattattt tctttaatga aagcaattta tactgttgtt
     1861 gggaattggg gctggtcaat tgttcttgtg actgtcctaa ttaaattggc attttatcga
     1921 ttatcagcta caagctataa atccatggct agtatgcgga agttgcagcc taagttgcaa
     1981 gctttgcgtg agcgatatgg tgatgataaa gctaaaataa gccaagctac catggaactt
     2041 tataagcaag aaaaagtaaa tcctttaggc ggctgtttac ctatattaat tcaaattcca
     2101 gtttttatag cgctctattg ggttttactg gaaagtgttg agttaagaca agctccattt
     2161 atattttgga taaatgattt ggcttcagcg gatccttacc atgttcttcc attaatcatg
     2221 ggagcaacta tgttgattca gcaaaagctc aatccagcac cagctgatcc aatgcaagct
     2281 aaggtaatga tgtttttacc catattattt acaggactgt tctggaattt tccttctggt
     2341 ctggttttgt actggattgt gaataatact ttatcaatat tacagcaatg gtacatcacc
     2401 agaaaatact cagatgaaaa gccagctaaa aaggttgttg ccacagcaaa ataaatgtca
     2461 atagatacca ttgttgctat cgctaccccg ccgggacgag gcggggtagg aatcgtcaga
     2521 atatcaggtc caaatgctta tgctatagcc ctttgcctaa atgggaacaa agcattgcaa
     2581 ccacgtttag ccactttttg ctctttatat aaaggaaata atgaagtcct tgatcaagga
     2641 ttggttcttt attttaaagg acctcattct tttaccggcg aggatgttat tgaaattcaa
     2701 gcgcatggct cacctgtggt tttggattta ttaattaagg aatccatagc agcaggggca
     2761 aggctagcca ggccaggtga gttttctgaa agagcatttc ttaatgataa aatcgattta
     2821 attcaagcag aagccatagc tgatttaatt caagccagtt cagatactgc ggctcgtatg
     2881 gctttaaaat cactgcaagg tgatttttct aaaaaaataa atcaattgaa tgaagagcta
     2941 atttacttac gcatgtatgt tgaagccgct atcgattttc ctgaagagga aattgatttt
     3001 cttaatgatg gaaatgtcag tcaattacta caaagaatta ttggacgatt agaagagata
     3061 cgttcacaag cgaatcaagg agttttgttg cgtgaagggt tatccttggt tattgctggc
     3121 aggcctaatg caggaaaatc aaccttaatt aacaacctgg cagggcgaga tgttgccatt
     3181 gttactgaaa ttgctggtac tacgcgagat attatgcgag aacatatcct attggatgat
     3241 atccctctac acattatcga tacggcagga ttaagagata gcgatgatct ggtggagaaa
     3301 gagggaataa aacgtgcttg gcaagagttg aagcgagctg attgcgtgct tcttgtagta
     3361 gatattaaca atccggatca acaaaattct ctgcttaatg aattgagatt aacattaccc
     3421 aataaaatac ccataatcac ggtatacaat aaaattgata ctactaaact tacagcaaaa
     3481 tgtgatgagc atacggttta tttgtctgca aaaacaggtg agggactgga tgagctaaaa
     3541 aaagtgatca aacaagtggt tggatatcaa ccaacagaag gccaattttt agctcgcaga
     3601 cgacatctac aagcgttgga tgaagcaaaa gctttgttac taactgggca atcgcagcta
     3661 acaaatcata aagctggaga attgttggca gaagatttac ggttagctca tcagacttta
     3721 tgtgaaatta ctggtgagtt cacttcagac gatttattag gaaaaatttt ttccagtttt
     3781 tgtattggaa aataacgatg atgcaaggct gtcaacatgt gaattttcaa tttttcttct
     3841 tttgaataca gtaaaattat tattaaacat tctgtggtga tgtgctttat tgggtcaata
     3901 aatttattct tttagtttca gcacatgtca aatgaaccgg cttttatctt gattcattat
     3961 tatacaaaat cagtttgaat cgcaaaaatc aatgtgtaaa atattaatac aaaaaagctt
     4021 gtcaaaatac atatcaaatg gtaacgtatt attgtaaaac ttcctgtgta atttttgtga
     4081 gttcttaata tggtctactc aaaacaaagt gctgcatttc gtagggataa acaacaattt
     4141 ttcgcccaaa ttccgaagga tagtaaactg gacacaaaaa ttgtaacaat tgatgatcga
     4201 gtaaacacta tagattacac ttttttacta caccacaaac aggatttaga agctgaattt
     4261 actcaaatgt ttactgtgtt acagcagcaa gacgatgaaa ataaagaaat tttctggatg
     4321 tattgctact attgtgcgtc cttgctggag gctttccaca aagcttattc acaaaacagt
     4381 aaacaagctg aatacgctaa aataaaacaa aaaataaaag accatttaaa taaggtcaat
     4441 gtccaagacg aagaacctgg ttttattcaa tccttgtacg attcctttct tggaggtttt
     4501 cgcaatttaa tcagctctcc gttgcatgct tccaaaatca gagattatgt tgcctactct
     4561 aacttgtgtc gtatctattg gacgttttgt cgtttgactt taactaatgg attgagcgta
     4621 gctcgagacc taaaaattat tgagaagttg gatgctattt taggaactca tactgatgtc
     4681 gataagatta tttcaactct tcaagcacct actggagtta taacttactt tagtgttggt
     4741 ttctttttga tgcgcttaat aattgatggc ggtatgctat taagacatac ctttttccct
     4801 actgaattgg aaaagggcgc agaaaatggt tgtgaaatcc atcaaatgga ttatttgcca
     4861 ggcccagcca gtctcgaggc atatagaaaa agttatattc ttgttcatga agacaatcaa
     4921 gaagtatcac tctattacat tcctagaagt ggtaaagcag aaaaattaag cattaaagaa
     4981 aataacaaac tgaaagatgc tttattgaag aaaatggata aggacacatc atcccttcgt
     5041 ttgaattcaa ctgaagtaga agagataatc actcatcaaa caggacatgt tcccgaagtg
     5101 actaccgcat gggacagatt taaacatgag ttttataaac gacactgtaa ttttgccaat
     5161 gattttgttt gggctacggt caacctgatt accaactata attacctttt taatatttcc
     5221 ggtcctgcag cagggatgat cacttctgtt tttctcgggt tcgatgtatg catggcttta
     5281 tataagtgca acttagccaa acaagagtat ttgatcaaga aagctcaata tttgcaggaa
     5341 atagaagact ataataatcc ggaaaaattc aaaaaaatga ctcctgaaca gcgattatta
     5401 catatagaga tgctcaataa gcaacttaga gagcttgaaa ttaattataa aacccaggag
     5461 gctactttct actttgttgc tgctgctgcc gccttattga tgatgggatt tactgcatcg
     5521 atgctggtta gtccgcctgt attagtcgtt ggttgttttt ttgcctgtac tcttgctgtt
     5581 gcaatgtatc tgtctacagg agcttattcg aaatacgagg aaaaaagttt gtatttggaa
     5641 gaggctcaat tgacaggtaa aaaccttcca gttgctctta aagaatatga agcagctcgg
     5701 aatgatttta tttttaccat ggtaaaaaat acggtcatgc ctatagtttt aattacaaca
     5761 tttgcaattt gctggcctgc tgcaattgtt ttaacagcta tgtacattgg ttatgaaatt
     5821 ttccatgctt atgaccaaca ctctgaagcg aaagcggcca aacagttggc tttagccgcc
     5881 ccttcagagg aaaaagatgg atatatggcc ttagccactc ccaattaggt ttcagggtac
     5941 attgctttga tggcagcgat atctttttca cttaaatgag ttcgctgccc tatttcaact
     6001 ccatcaacta gaggtatgat agttggctga ccattcttgg aaaaagcaaa tcgaccataa
     6061 tgcataatgg atccatagtc gtattcaccg aagtctttcc catcagttaa atgttggtca
     6121 aaattaaatt tatgctcttc atcaatattt tcccagacaa tacgaatata ttgatttctg
     6181 tctgttcggg attgctcatg ccacattccc aaggcatgtc ctatttcatg taccgtattc
     6241 atggtagtac atcttggcgc caaattgatc tcttgcttcc ctccctggcg tccaacatag
     6301 gaagagcagg ttgtgccttc tgccgggata aaagaaatgt aatcacgata gtcatagcga
     6361 tttttagaag ttaattcaac gaattctaag ttcgtgtttt tctgccaatg atctattgct
     6421 tgtaaaatag caagcttgtt tataaaaggt aaattttcag caatttcata agggataaca
     6481 ccatttggcc agcgtgaacc tcctactttt ggtgtaataa cagcaccttg gtttttaaga
     6541 ttctctgctt taccaatcaa aatatcacct tccactactg caaattctcc aacttgttca
     6601 taggtaatgg tacgactgcc cattaatgga tcagttattg tgatttggtt gagattaatc
     6661 gcgtaagaag aggagaaaca taatgaggaa taaaacaaac ttaaaatcca tgatttaatt
     6721 ttcatatata gtccttaagt ttaaaaaaca aggacagttt aggggaaaga gggattagat
     6781 ttccagaact ttttatatag tttgttgact catgataacc ttaaagcaag gacgccttta
     6841 caaaacgtcc ttaattgata attatgtgcg attacatgaa taaatagcta taagaatata
     6901 gcgatgcgaa agtatataag gctaagttta aatatttttg caaagagtcg gtgtagtgtt
     6961 gctaacttga gcactatctc cattttcatt aaagaacttt aaatgccact ctggttttct
     7021 attttttcct tttattgatg atttagagga tttatattcg tcaggcaccc aagttatttg
     7081 agcaatatcc ttaactttct caattttctc agtttcttct gcaatctcct ttttcagggt
     7141 cgctatgata ccttttggca taatatttac tttttgtgtt ccgtctttat caaaactata
     7201 gagattacct tttctgtcta ttccgtatcg atgattaaaa attccctttg aagttttgca
     7261 gaccgttgta aacatgtaat attcattgct agtggctctg ctgggacgta ttagaaaagg
     7321 taattgtttt ttattcaatt cactttctgc attgttaacg ctggagacaa agaatttgca
     7381 ggaacgcaat tgagagcata agcttttttg tttattctct ctttgagggg actttgtttt
     7441 tttagtcatt ggagtaaata attcagaggg atttgtttca gcttcttttt ccccattttc
     7501 ttgataatca aggtcgttat tttttttctc aggttgggat ttccatggaa ttttgctgag
     7561 attctccgtt ttttcaataa tcatttttgt tataactatt tctctttcta atgctgcaat
     7621 tataccttca gaatcaatgt ccattttttc aatttgtgaa tcaaaaaatt tataaagctc
     7681 gccttccgta ttaataccat atcgattggc aaagattcct tgcgaggttt tgtaagcggc
     7741 tgtaaacata atgtaaccag aaatttggga tttacttttg cgaattacaa aaggaatttt
     7801 ccctttgtca agacagctaa cgacatcatc aggatctgaa acaaagaact catgttctaa
     7861 tatttggcgt acggcaggta tttcggaaaa tttatcattt tggtttattg tttccgcaaa
     7921 cgcatcaaga ttatacacat gctcatgcat gtctttgact atttgccggt aattcttgta
     7981 cggacgtccg gttcccttta tgctggcgta tgaactgaca ttgctgccgt cataatggtt
     8041 taaacgtaaa gtaactccat gaggaatcat ataagaataa tttataaaat atttttttaa
     8101 ggtggataaa atgtcatctt tgtcatcaat aaaattaaag attatatctt catcaggatg
     8161 atcatttctg attttttgta tttgcgcaaa aaggagaatt cttttaaagg gatctaattt
     8221 tgcatgccaa ttttcattat aatcgggata aggaacatgt ttatgttgta gttttttatt
     8281 gtctaaccat tcaatattta atacttcatc cataattcga ttaaaggtcg ttccatactc
     8341 aagatctcca gtgatatcag gtaagagcgt tttatctaat tcaaccttga gataatcgca
     8401 tagtttttgt aatgctaaac aaaaagatcc tctaaatagt tttttttctc gaagagcgtt
     8461 gagttgttcc ataaacatgg attgtcgact ggagccgtta aaaacgatga ttgggtgcaa
     8521 tttgtttttt gcaatttcat gcttgatttt ttttattaat ggtaaatttg tataaatgac
     8581 cgctttacct gaaaattttt cagagtcagg agcgtcatcg tattcttttc ttaaaataca
     8641 ctcatcacca tcaatagaat aacattctat cgtcatttca atacctcaag ataatttaat
     8701 ttatttgtaa aaaagatgat tagcaggata ttaatttgaa ctaaatggtt gagaaagact
     8761 taaggatgga ctttaattat agttgttttg gcaatatgtt attgtgtgtt attttgatgg
     8821 aaagaagcct gattaactta aaatacttat ctcaataata tccagctatt ttgatttaag
     8881 attgactaag tttaccagtc ctccactcac ttacatcagg cacaatgcgc ttcttaagtg
     8941 aataattaat tgactggtaa attttaattt atttttatta aggaattatt aaatagagag
     9001 aaagttttgt gtagctattg ttcaggttag ttaatcggat tagaccaagt ttatcgcgaa
     9061 caagagtatg cctagctttt ctgggccaat gaagcggtac tatggcagga gaaaattatg
     9121 cggaatcatt aatgaataga ggccgctatt ttaattccat tagaaatgtg aaaaactgga
     9181 tttttctctt gtttagtaaa ataatccttt agtcttgaaa tgaatgagtg agaggtagga
     9241 tgactcgaag tcaattgatt ttggctattt tagcaggctt ggtcataagt attgacaata
     9301 tgattagtat tgttgcattt tcttcactta tttatcaagg ttcattagag atttatattc
     9361 cgcttgtcat taatatattg gcaatttcag taattattat aagcgccagc tatttgttca
     9421 tgtcttcccc tccttatgca atcgctcagt tgcaagatga agcggcaatt ttgtattcag
     9481 ccatggcttt agtgataatt aaatcgatgc catcaagttc tacgaatgaa gccatcttta
     9541 ttacgatcct ttttatgctt ggagtcacta cctgtttgac gggcttatca ttctacttcg
     9601 ttggaaaatt taggtttgga aatattatcc gttatctgcc atatcctgtg atttgtggtt
     9661 ttatggcagc gacaggctgg ctagttttat caggaacatt tgcattgtta actggtaaag
     9721 ctctgcatca gaattggctt atcttttttc ataaagagga aatattattg tgggcgccag
     9781 gttttattgc gggaatagtt gtttatattt ttaaagaaaa attaggttat cgatacgcgc
     9841 tgcaattgat gtttatttct cttgtaattc tattctatgc tcttttttat atttttaatt
     9901 tggagtattt ggtcgatcca accgaagggt acgtcttggg acctttcaat caaagccata
     9961 ttgattcgct catgaaacct gaaatatggc aaatgattca ttacccattc tcaatgaccc
    10021 tattgaatta catagggttg gttatactgg taagttttat tgccttgtta ttaaacgcaa
    10081 gcagttatga attaatagtt aataagcaat tggatttaaa caaggaactt cgtatagcag
    10141 gtattggcaa tgtattcagt ggattacttg gaggaatggt tggctatttg tccttgtcag
    10201 cgtccactgt tgcaaaagag tatggcggcg cccgtattat cggggtgatg gctttcgttc
    10261 ccatgttgct tattttgtgc tttggtgctg tcgtcgttga atgtttcccg aaaatggcta
    10321 tcggctgtta tttgttttat atcgcttttt gctttctatc tgaatggttg atcaatacct
    10381 ggcgtttgtt aaatttaagt gaatatttaa tcgttttgct gatattggtt attgctattg
    10441 tctttaatat gattacggcg gttgctatag gaacggttat ttcaattttt atcttcgctt
    10501 ttcgctacag caaaataaat ccggttaagt atttattttc cggctcagat tataactcct
    10561 cctttgagag aaatccgata tctcaatcca ttttatcgac acagggagat gaaattcagt
    10621 ttttgaaatt gcagggattt cttttttttg gctctattta caggttactt cagttaataa
    10681 aaagtataca gaaagctcga tatatcattt tagactttga gcttgtctac aatatcgact
    10741 catcccgcat tattttaatg aaaaacttaa agctccatgc ggaaaagaat aatatttcct
    10801 ttgcaatttg ttcattaaag cctattgttt atcatgaatt aatgaaaacc aatttattac
    10861 aatcgaaaac caattggctt aaagtcttta ctgatcaaga atcagcttta gagtggtgtg
    10921 aagatcaagt tatagaaaaa tataccgaaa atataaatat tgaagaagtt acacttgaga
    10981 aacagttact ctatttaggt ttttcctccg aactggtagc atggattagt gaaaaggctt
    11041 ctataaaacc ttataactct gatgatatca tttgtcatga aggagaagag tcaaacagtg
    11101 tttactttat tcatcgggga aaagttacag catttgtcgg tgataaacgt gtacttgttg
    11161 tgggttcagg taatgtactt ggtgaaattg ccatgtacac tcataagaag cgcacagcaa
    11221 cattaaaaac caatgaaaaa accatcgttt atgaaattga tttgaaatca atccaggagc
    11281 tttctaaaaa taaaccccta ctcttggttc aatttcatat gtgtatggct aaaattttgg
    11341 caaatcgtct atcggtacta aataaacgaa taaaagtttt tgattccacc gccatattgc
    11401 aataaaagtt aataaaatat tcttttactg tgaaataata ataaggaaca acgttttaaa
    11461 gttgttccaa ttgtcttaac tgaattcgat aggcaacaac tctcttgtcc atgatgtaat
    11521 gccagagcgg tggaattaaa gctatgataa tcattccgag gtaccctgag ggaagttgag
    11581 ggctgttttc catatgacgt aaaatttgat agggtcgtgc accatgggca tggtgatctg
    11641 aatggcgttg taaatgaatt agcagctgat tacttagcca atgattagca ttccatgaat
    11701 gatttggatt taccttttca tattggccgt tactcagcat tttcctttcc agtccatagt
    11761 gctcaatata attaacaatt tctaatgtca ggatggcaat gaatgattgt aataagaaaa
    11821 atgacagagc gattaatcct ccaaaaatga aacagactat acaaatgatg aatggaacag
    11881 taaggatcca ccaaaaatta ttatgtatac tccagatgga ataattttta tgcgataaac
    11941 gcttttcttc caaaagtaag gcagattgaa acgaacctaa taatgttttt ggtaaaaatt
    12001 gatataaatt ttcgcctaat cgtgctgtgg cagggtcatc aggcgttgct actttaacat
    12061 gatggccttt gacatgctcg ataaaaaaat gaccatagca aactgtaact aataaaattt
    12121 tactcattag ttgttgcaat ttactgtttt tatgcatcat ttcatgagca aaattgatgc
    12181 ctactccacc tgagataagt cctagtgaca tggtaaaacc taaccattca ttccaaacca
    12241 aattttgagt actaactata tagactccat aagcaatgat ggttatttgt ataggcacat
    12301 aagcacaagt aatatattta aagaacttat cttttaggag ttcctgttcc tgctcttttg
    12361 ttggatttac cgaatcatga agaaaggcat caattaatgg aataaaagca aaaacaatta
    12421 aaaagggtag aaaactgtac cagcctccta aatataaaga gacaaagggt aatgggataa
    12481 gtaaatagac cagtaaaaaa ctatattttt ttaaatcatc cattttataa ttccaagcat
    12541 agcctaatat agaaacaata tagcttaaaa ggcgatatct ttgcttggcc ttagaggaca
    12601 aaaaaaggtc atttacggac aatgttatgg gaataagcga tcaatattct ttttagctcc
    12661 cttttaattt caatgcctct tcatagagct gatttttatt ggcattagtc aatctgcatg
    12721 cgatagccac agcctgcttt aaaggcaact cttccaacaa tatcttgagt agttcttcat
    12781 gtgaattgag gtctttattt gttgagcgag gtggaaaaat caaaacaaat tcacctttga
    12841 tatgaccagg ttcactgagc agccagtctt taatttcttt tatttttcca gatacaaatc
    12901 gttcaaaggt tttggttatt tcttttgcta aaacaagttc ataatcttgg ccatagatct
    12961 ctgcaatatc gttaagacaa tctatgattc tgtgagttga ttcatagaat actatggtat
    13021 aaggtactga ccgcgatgat tccagggcat gttttctggc actttgtttg gccggaagaa
    13081 aacctaaaaa ggcaaaagaa tcgcagggta ccccggcagc actcaacgca gcaattaaag
    13141 cgcatgcgcc aggaacaggc acaacaggga tatggtgctg gcgagcctgt ttcaccaaag
    13201 gaaatccagg atcgctaatc aagggcgtgc ctgcatcact gattagtgcg attgattttc
    13261 catcgagcag cagctcaatt atctcattgc ttttgttcgc ttcattgtgg gcgtgcaatg
    13321 aagttaaatt attttttatc cctagcgaag tcaataattg catggaatgg cgtgtgtctt
    13381 cagcaagtat taaatcaaca cttttcaagg tattgagagc cctaaaagaa atatcttcgc
    13441 gatttcctat tggggtagca actatataga gagttcctaa acctgttgct agagtgtttg
    13501 tcatagttta tcctcttgcg aaaagcaaat ttggcgaatt aacatgttaa ttaaacaatt
    13561 aaaacttcgc atgttcttag tattggtatc tctttttttg ctttgccaat gcacaaaaac
    13621 agttaactca accgagcctg tagtaacagt taataaaaaa ttaaaaaacc catattcatt
    13681 acctactgca gcatacttgg ctatggctaa gagtcaacaa ggccatgaaa agcaaagcgc
    13741 attgatttcc gcagctggcc gtttaattac tgatgggcaa tggaagcaag gaactgccat
    13801 tttagcacaa actggagaat taacagttga gcaggctgat gaaaagaatc ttttactggc
    13861 aaaagtcgac tttatgcgcg atagacccag agatgcctta gccaagttag ctagaattaa
    13921 agaacctgaa agactctcat tatataatca aatccaattt cacgaacaat tggcgaagtc
    13981 atttcggtcc attggtagtt actctgaatc agttagtgag cgtatcaagt tagaatcatt
    14041 gctccctgat gaggaaagtc aggtcaataa tcggcgggct ttgtggttga ccttaaccag
    14101 tttacctcaa gaggagctga atacgatggc tctggaggct gtgaacaaat cagaaatgca
    14161 aggctggttg cagctggctg ttatttcccg caagtataga aataactcaa agtcgttgct
    14221 ggctgccttg gatcaatggc agatgcattt tagtaatcat ccagcaaatc agattttgcc
    14281 taatccctta gacagcatat cagataaatt attagcccca cctaaacagg tcgctttgtt
    14341 attgccttta agtggtccat tatccgggcc gggtaatgct atacgggaag gttttatggc
    14401 agcctataag gccaacaggg gagatgaatc aacaaaaatt aaagtttatg atacaagcaa
    14461 aggcgatata acgaacacct atcgccaagc tatttctgat ggtgccgaat atgttgttgg
    14521 ccctttaacg aaaactcaag tcgctaccat tgcatcaatg gagcatccgg ttcctaccct
    14581 attgctaaac gatacggatg ccagcacaca aaataattct tattccttag gcttatcccc
    14641 tgttaatgaa gcgattcagg ttgcaataaa agcaagaagc aaaggatata aaaaagcgct
    14701 gattattgct cccaataatg cctggggtaa tgaagtaacg aaagctttta tcaatcagtg
    14761 gaatgaagat gggggccata ttgttgactc tcttcgctac gaagccaaac aagatttgaa
    14821 taagagcatg aaagattttt tacaaatcac caatagccag gaacgcgaga aaaaactaaa
    14881 gcaggtatta ggctataatt tccaatcgac tacgagccga agacaggatt ttgatatgat
    14941 atttttattg gcttatcctt ccaaggcccg ccaaattatg cctttattaa aatattatta
    15001 tgccggagat gctcctgtgt acgctacttc aagtgtttat ggcggtagcg ccaatgcttt
    15061 gaaggataag gatttggatg ggatcatttt ctgcgatatg ccttgggtat tctctcatca
    15121 gatgggtacg aaaaactggc ctgaacaatt caatagctat aacaggcttt atgctttagg
    15181 tgtggacagt tataccttgg ctacccaatt aaaccaactg atattatttc cagccgatgg
    15241 ctctagcgat tcaacgggta ttttatattt aaagccgact caacaagtgg ccagggttct
    15301 tgaatggggg caatttaaac agggtttagc ccattcacta ggggatactg tttagttttg
    15361 aagtgagcca agaataatga cccaagaaaa aggaaagttt gctgaacaat tggctttaaa
    15421 ttacttaaag gaaaatggtc ttgcattagt aatgcaaaac tatcattgcc gtttaggaga
    15481 aattgattta attatgcgtg aaggatctta tctggttttt attgaggttc gttcgcgttc
    15541 aaatatgaat tttggtgggg ggcttgccag cattacttac gaaaagaaac aaaaaataat
    15601 aaaagcaact tctcattata tgattaagta tcgcatacaa gataagtttc ctatacggtt
    15661 tgatgtgatt agtatagatg gaaaatcaaa caagattacc tggttaaaga acgcatttga
    15721 tgcaggatgc taagaagata atgaatagaa ggttttgaaa tgattcagtt agaagaaaga
    15781 gtaaggcatt tatttggtgt cacaatagaa gcaaaaatat cagcagctga tatgttgtcg
    15841 ggttcaatag ctaaagcagg gaagttgttg gttaactgtt tattaaacga tggaaaaata
    15901 ttcatttgcg ccagtggggg atctagtgct aattgcatgc atttttccag ttcgatgcta
    15961 aatcattttg aagtggaaag accttctttg ccggtcatta atttatgcgc tgatgcgtcc
    16021 tgtttgagtt catttgccga tgagcatcac tttggtcaag tgtttgcaag gcaaattcaa
    16081 gccttgggcc aagagcaaga tgtattactt ttactaacta catcaggtaa ttctgatagc
    16141 atgctgcaag ctcttcacgc agcaaatgag cgcggtatgg ataccattgc actaagtggc
    16201 cgcgatggag gtgttttggc gaatcatatg gggccagaag atatagagat aagagtagca
    16261 tctgacagta ctgcattgat aagagagctg cacttgttca tattgcattg tttttgtgat
    16321 ttaatcgaac aatctttatt tggccaagtt ttaggataaa aaatgaaaat gaatctaaaa
    16381 tttaaatcca tcatagtgat gatgattggt gctttactga ctggttgcgt tgctgtggtt
    16441 gttgcaggtg ctgcagcggg aatggtttat gatagaagaa gcgtaactac catggaggcg
    16501 gacgcccgat tatttcatgt gattcataaa accatcatca aggaccctcg atttcggcac
    16561 tccagaattc ttgtttccag ttttaaccgg gtggtattgc tggttgggca aactcccgct
    16621 gcatcattac gcgttctagc cgagcgaatt gcccaaaatg cccctggagt taaaagagtg
    16681 tatgatgaaa taaccgttga taatcccatt cccttgactc aacggacaaa agacagttgg
    16741 attacaagtc aggttagaag taacatgttg actaaaaaag gacttgaatc gggttcaata
    16801 cggatagtca ctgaaaatgg agtagtctat ctgatgggaa ttgtttctga cgagcaagca
    16861 ttgctagctg ttgatgtggc aagacgtgtt gcaggagtga gaaaagtcgt gaaaatattc
    16921 caatatatac gttagcatgt ttaaacacgg atgttttata ctaatagtct tgtttttagc
    16981 cagctgctta tcaggctgtc tgggagcact atggactggg gcaactctag cttatgatag
    17041 gcacaatgta tataaaaaat tagatgatta taatttaata aaagctctta atgatgtttt
    17101 agctgtcaac cggacattta aaaactcaga gacagtactg gatattgctg tgttcaacag
    17161 agatatttta atagcagggc atgttccaac ccaggagttg tatgatgaat tacaattacg
    17221 tctaagcaaa gttaaaggat acaggcggtt attcaatcgc gtaattatca ataaaatgcc
    17281 atccaactcg atccaggaca gctggattac cacgaaaatc cgtagccaaa tttttgcaga
    17341 ctcttcaatc gatcctaacg ctttcaaagt ggttacttct gatcgcgttg tctatttgat
    17401 gggagatgtt cagacagaac aagctgaaaa agtgattgaa attgcaagat acacaagtgg
    17461 tgtacagaaa gtaataaaac ttatgcggta ttatacttat caaaccagta cgaatatggc
    17521 ctgatcagaa tcaggccaat agctgcacgt ccaatccagg aagtggctaa cctgggagga
    17581 taagtatact gtatcaatgg aatcgacctt tatccagtcg ttttcgtatt tttctcaaat
    17641 taaactcgcg taacagccct gtagagccat ttacaatatt ttcttcgtct ttattgttcg
    17701 tttgattgca tggatgagat ctgcaatgtt gtcgatatga aaggtgattt ttattatgct
    17761 tgtgtgaaag cccatcgcta taacgattgc tcattttttc ttgcagcctg gctgctgtat
    17821 gctgaatttt ctctgacatt ttttaatcct taggcgaggt taaatagtaa atcgcttaac
    17881 atgtgattaa tgctaaaccc tacattacaa ttatagctcg taatttgcat atcaacgttg
    17941 attattacaa ttgtttaatt tataattcgc gccgtgaaag ttaggtgaat atgtgaacaa
    18001 acagctgaat aagcgcggaa tcacgcgatt gtggctggcg cagttgggag ttactgtaat
    18061 ccttgctggc ctttgcgcaa ttttatttgg tgtcaatgcg gccagttctg cattgcttgg
    18121 tggactggtt tgtattatac caaatgctta ctttgctatt aaactgttta agcatcaagg
    18181 tgcgagagct gcgaggcaaa tagttaatag tttttataag ggtgaagcgt taaaaataat
    18241 attgtccatg ttcctgtttg cattagtatt tttattatgt aggatcacac cggcagcgtt
    18301 ttttgcatca tacattttgg ttcttatgac gcattggttt gccccatgga ttattgtcaa
    18361 taaacagaat aggccaaaaa gtgactgaaa tggtatctag cacaaactat attaagcatc
    18421 atttaacgta ccttacctat aacgtaaatg atatgacatt cggttcaagt ggtggttttt
    18481 ggaccttgaa cctggataca atcttctttt ctatagcctt gggtctaatt gctctggcaa
    18541 ttatgtattt agcggcaagg aaagtaacca ctggtgttcc tggaaagtta caaaattttg
    18601 ctgagctcat gctcgagttt gctgataatc aggttaaaga ttgttttcat ggaaaaaata
    18661 acttaatagg tccattggca ttaactatct ttatgtgggt attcctgatg aattttatgg
    18721 atatcgtgcc agtagatatc cttcctgtac ttgcgcagct agtcggtata cactacctga
    18781 aagttgtacc aactaatgac ttgaatttga cttttggatt gtcactttcc gtatttcttc
    18841 taatagtttt ttacagttta aaaataaaag gcgttaaagg ttttgttaaa gagctcaccc
    18901 tgcagccctt caatcaccca ctattcattc catttaattt attgttggag ttggttgggc
    18961 tgatagctaa acccatttcc ttggcattac gactttttgg taacttatac gctggcgaat
    19021 taatatttat tttgatagcc ttactaacct taaatgcaac aacgtcatca ttaattagta
    19081 ctatgacact gggtacggcg caatttattc ttgcactcgc ctggtccatt ttccatatat
    19141 tagtgataac cttacaagca tttattttta tggtactgac tatagtttat ttgagtttgg
    19201 cccatgaaga tcattaaccg atttaattat catcatccat tgtaaagggg ataaaatatg
    19261 caagctgctg aattaatagc acaagttcaa agtatgaccg tcattgcggt tgccttgttg
    19321 ataggtttag gtgcgttagg cacagccata ggtttcggat tgctaggcgg taaatttctc
    19381 gaaggctcag ctcgtcaacc agaaatggta cctatgttac aagttaaaat gtttattgta
    19441 gctggtttgc ttgatgcggt aaccatgatt ggggtaggta ttgcattatt ctttaccttt
    19501 gctaaccctt tcctaagcag cctgggttct tgataacaca caaatagggc gctatgcgcc
    19561 ctcactgtta caggagaata tactttggat attaatttaa cattaatagt tcaaatgttg
    19621 gtttttgccg cgtttgtact gttcactatg aaattagttt ggcctccact agcaaaagca
    19681 cttgaagaaa ggcaagataa aattgctgat ggattggctg ctgctgaaag agggcgtaaa
    19741 gagttggagt tggctcaaca tcgtgtaaaa gatgagttaa aacaagccaa ggctcactct
    19801 gctgatatta ttgataaagc gaataaacga gcatcagaaa ttattgaagc agcaaaagag
    19861 gccgcaaaac gcgaagctca aattcaagca aaactcgcgc aagaacaaat tgctcaacaa
    19921 gtaaatcacg ccaaagaaga actgagaaaa caagttgcaa agctggctat tactggggca
    19981 gagaaaattt taatgcgaga ggtagatgca aaagcgaata gcgaactgtt agataaccta
    20041 atagaagaga tttaaaatgt ctgatagtac cactatagcc agaccctatg caaaagcgat
    20101 atttgaacat gctttagcag aaaaaaaact gagtgaatgg tcagaatatc ttacattatt
    20161 ggctcaagtt gttttaactc ctcaagctac tcagtttatc gctaatcctg catcaacaga
    20221 tgaacaacaa attgaacttc ttattgagat atgtggttca aagttcaaga aaaatgatgc
    20281 actaaataat ttaatcaagc tattaacaac caataaaaga ttgatgttgt tgccagaaat
    20341 taaagctctt tatgaagtat atagagcaga gcaagaaaaa atactggagg ttgatgtagt
    20401 cagctattcc gaattgactc cagcgcaaca acagcgtttg agcgaatcct taagtcaaag
    20461 attatcacgt aaggtttctt taaaaataag tattgatccc tccttgcttg gcggagcgct
    20521 aattcgagca ggcgatttag ttatcgatgg ttcagttcgg ggcaagctta atatgcttgg
    20581 caccagcttg gccgcttaat ttagaggata gtttccatgt cagaacaagt agcgttaaat
    20641 ccttctgaaa tcagtgaatt aatcagaaag aaaatagatc aatttagtgt ggtttctgaa
    20701 gcacgaaatg aaggtactat cgttagtctg aaagacggta ttgtacgact gcatggtctt
    20761 gccgatgtta tggcaggtga aatgatagag tttccaggag gggtctatgg attggctctt
    20821 aacctggaga gagattctgt tggtgctgta atattgggcg actcatccac attagctgaa
    20881 gggcaaaaag gtaaatgtac aggtcgtatt ctggaagtcc cagttggaaa aggtcttttg
    20941 ggacgtgtcg ttgatgcttt aggaaatcct atagacggga aaggcccgat agagtcatct
    21001 ggtatgtctc caatagaaaa agtggctcct ggtgtaatta cacgtaaatc agtagatcaa
    21061 cctgttcaga caggattaaa agcaatcgat gcgatgattc ccgttggaag aggtcagcga
    21121 gaacttatta ttggtgaccg ccaaactggt aaaactgcca ttgctattga tgcgattatc
    21181 aatcaaaaag gtaccggcgt taaatgcgta tatgtagcca ttggccaaaa ggcttcatcc
    21241 gttgcatcga tcgttagaaa attggaagag catggcgctc ttgagcatac tattgttgtg
    21301 gtagcgggag cctctgattc agcagcactc caatatatag caccttactc aggttgtacc
    21361 atgggtgagt atttcatgga gcgtggggaa gatgcattga ttgtttacga tgatttaact
    21421 aaacaagcat gggcatatag acagatctcc ttgttattac gtcggccacc aggtcgcgaa
    21481 gcttatcctg gtgatatttt ctatttgcat tctcgcttgt tggaaagagc ggcaagaatt
    21541 aatgccgatg aagtagaaaa attaactaat ggggaagtga agggtaaaac aggatcactg
    21601 actgcattac cgattatcga aacacaagcg ggagatgtgt cagccttcgt accaacaaac
    21661 gtaatctcta ttaccgatgg gcaaatattc cttgatgttg atttatttaa ctcaggtgtt
    21721 agaccagcaa tcaattccgg actatccgtt tcacgagttg gtggtgctgc acaaacgaaa
    21781 atcatgaaaa agcttggtgg tggtactcgg cttgctctgg cacaattccg tgagttagaa
    21841 gcattctccc aattcgcatc tgatcttgat gatgcaacac gtaagcagct ggagcgtgga
    21901 caacgtatta cagagctgat gaaacaaaaa caatactccc cacttacagt ggctgaaatg
    21961 ggggtaagct tgtttgtggt agaaaaggga tacttggacg atgtacctgt aaatgaaatc
    22021 agctcgtttg aagcttcctt gcacgattac atgcgcagta cccatgccgc tttattacat
    22081 gcaattaatg aagccggtgc ttatgataat gaaatcgaag caaaactgaa aaaagcagta
    22141 gaagagttca agaacacagg cagttggtaa ggtatagcct caaagagtta gctctttgag
    22201 gcacaaaggc cattatttga aagtaatggc ctctaaaact aattaaggcg aattgaaaga
    22261 tggctggagc aaaagaaata cgttcaaaaa tttcgagtat taataaaact cgaaaaatta
    22321 ctcgagctat ggaaatggtg gctgcaagca aaatgcgcaa aacgcaagaa agaatgcgcg
    22381 cttctaaacc ttatgcgaat aaaatctatg aagttataaa gcatattgct cgagcagcat
    22441 ctgagtacag acacccattt atgagtgagc gcgaaatcaa gcgtataggt attattgttg
    22501 ttaccacaga tagaggtctt tgtggtggtt taaactcgaa tctatttcgt gaaacaattc
    22561 gtactattcg taattggcaa gaacatggca aggaagttga tatcgctgtt ataggaagaa
    22621 agggccaggc cttttttagg cgtgtgggtg gtaacatatt aggttccata gatcatctgg
    22681 gtgatacacc gagtatcaat gatttcattg gtgttgtaaa aataatgctt gatgcttatt
    22741 acaatggcgc cattgattct ttgcacattg tatacaatga gttcattaat acaatgacgc
    22801 aaaaaccttt tgtaaaacaa ttattgccgt taccaaaatc agaagaagat aaaaaaacat
    22861 tgggtcatca ttgggactat atttacgagc ccgaagctaa agaattatta gatgaaattt
    22921 tagagcgata tattgaatta caagtgtatc aagctgttgt agaaaacatt gcttgtgaac
    22981 aagctgcaaa gatgatagca atgaaaagcg caacggataa tgctggtgat ttgattaaag
    23041 aatttcaatt ggcttataac aaagcccgac aagcagccat tactcaagag ttggcagaaa
    23101 ttgtcggtgg cgcagccgct ttataaagag ggtatataat gagcttagga actgtggtag
    23161 aagtaattgg tgcggtagtt gacgtagaat ttcctcgtga tagcgtgcct aaagtcaatg
    23221 atgcgttaaa gcttgttgat agtgatctgg tttttgaagt gcagcagcaa cttggagacg
    23281 gagttgtgcg tactattgcc atgggaacaa ccgatggttt aaagcgagga ttaaaagcag
    23341 aaaatacagg ccatcctatt caagtgccag taggtaagaa aactttggga cgcattatgg
    23401 atgttcttgg gcgtcctgta gatgatgctg ggcctatcga tgctgaagag acttgggcta
    23461 ttcatcgtaa agcaccaagt tatgaagagc aagctggcag ccaggaatta ttagaaactg
    23521 gtattaaagt aattgatttg ctttgccctt ttgccaaggg aggtaaagtt ggtctattcg
    23581 gtggtgccgg tgtaggtaaa accgttaaca tgatggaatt aatacgaaac attgcaattg
    23641 agcatagcgg ttattcagtg tttgcagggg ttggtgaacg tacccgtgaa ggaaacgact
    23701 tctatcatga gatgaaagac tctaatgtat tggataaagt atcgcttgtt tatggtcaga
    23761 tgaatgagcc gccaggaaac cgtttgcgtg ttgctctaac cggtttgacc atggctgaaa
    23821 aattccggga tgaagggcga gacgttcttt tgtttatcga taatatttat cgttatacct
    23881 tggcaggggt tgaagtatct gcgctgttag gccgtatgcc ttctgcagta ggatatcagc
    23941 cgacattagc agaggaaatg ggtatgctgc aggagcgcat tacctccaca aaaactggtt
    24001 ctattacttc tatacaagca gtatatgtac ctgcagacga cttgactgac ccatcaccag
    24061 ctacaacctt tgcacacttg gatgctactg tagtattgtc ccgtcaaatt gctgaattag
    24121 gtatttatcc tgcggttgat ccgcttgatt caacatcacg tcagctggat cctttgattg
    24181 taggtcagga gcattacgac accgcacgtc gtgtacagca aaccttgcaa cgatataaag
    24241 aattaaaaga tattattgca attttgggta tggatgaact gtctgaggaa gacaaacgag
    24301 ttgtaactcg ggctcgtaaa atccaaagat tcttgtctca gccattcttc gttgcggaag
    24361 tgtttacagg ttctcctggt aaatacgtat cgcttaaaga tactatcaaa ggtttccaag
    24421 gcattcttgc cggtgaatat gatgatttgc ctgaacaggc attttatatg gttggaagca
    24481 tcgaggaagc tgttgcaaaa gctaaaacct tatgaggcaa gataatatga gtattacaac
    24541 gcacttggat attgttagtg cagagcatga aattttctct ggtgtggtgg aaatggtagt
    24601 tgcaactgga gaattgggtg aaattggaat tactcctggg catgctccat tactaactgt
    24661 gctaaggccg ggtgaagtta gaattacttt gcaaggtggt acacaggaca tatactatgt
    24721 gcagggtgga atgcttgagg ttcagcctca ctgcgtaaca attcttgctg atgtggcaga
    24781 acgagccgag cacttggatg aagctgctgc attggcagct aaagcgaaag ctgaagccgc
    24841 tatagccagt aaaggaggcg atattgatta ctctgttgct gcagcagaat tggccagagc
    24901 agtcgctcaa ataagagcaa ttcaaaagac caggaaaaaa atgaagtaag agtttcctgt
    24961 ttgtggaaac tgctttgcta tgaatacttt atacccctct acaaaacggg tttcttcctg
    25021 ataggaaaaa cccgtgtttt actctcttag taaatataat tttgtcgccg ttaatacacc
    25081 aagaaaatag tgtgataact ctaatgctta gcattgctcc attatttgag ggaattcaag
    25141 ttatctgcgg atacatagat atggtatcca gtatgcgcat tgccattatg gttgaaagac
    25201 aataccaggg gcaattgatc atttaaaaat caagtggtaa caactgaaat ttgaaaattt
    25261 gtttatagtg gagtgatcaa tttacaactt ataagaaatt cttggtaatt aacaacaagt
    25321 tatcttattt attccatatt cattcacata gttatccaca ggacacaaag atataaaatt
    25381 aaaagaaatt aataaatgtt aatataaatc aataagttaa atttatttca tacagatgaa
    25441 gcaaaaggaa tataaagtta tacacagatt atacgatgat tataattcta acagcatgaa
    25501 tattctgtta aaattgtttt gcattgtctt atatgattca ctaggatgat ggatagcaat
    25561 taatatatga agaaaatacc caatatgaat gaagtaactg aaaaaaaacc gcttactgag
    25621 tttacagaaa aagcttatct tgattattca atgtatgtca ttttggacag agcattaccg
    25681 agcatcgcag atggacttaa gcctgttcaa cgcagaattg tttatgctat gtctgagttg
    25741 ggtctgaaag caacagcaaa atataaaaaa tcagcgcgta cagtagggga tgttcttggt
    25801 aaattccatc ctcatggtga tagtgcatgc tatgaagcta tggttttaat ggctcaacct
    25861 ttttcatatc gctatccatt tgttgatgga caaggaaatt ggggatcccc ggatgatccc
    25921 aaatcatttg ctgcaatgag gtatacagaa gcaagattat ctcattatgc tgatgtgtta
    25981 ctcggagaac tgctacaagg aaccgtggat tgggtagata attttgatgg tactttgcaa
    26041 gagcccgctc ttttacccgc tcgtttgcct aatattttat tgaatggtgc aacaggtata
    26101 gctgttggta tggcttcaga tattcttccg cacaatttga ctgaagttgc taatgcgtgt
    26161 attcatctgc tggataaccc ccaagccgat attaagacta tttgtgaata tattaaaggc
    26221 cctgattttc caacagaagc agaaatcatt agcccaagtg aaacaattag agctatgtac
    26281 gagtccggta atggctcaat taaaatgcga gcaatttata ctcaagaaaa acaaaatatt
    26341 gtcataacag ctctgcctta tcaagtatcc ggagccaggg taatggagca aatcgcgttg
    26401 cagatgcaac agaaaaaatt accgatggtt gaggatttac gtgatgaatc agatcacgag
    26461 catccaacac ggttggttat tatcccccgc tctaatcgag tagatataga aagtttaatg
    26521 tcgcatttat ttgctaccac ggatttggag cgtagctatc gagttaattt taatatgatt
    26581 ggtcttgatg gcaagccacg agttaaagga ctgttagcaa tcttgactga atggttggaa
    26641 tttaggctta ccacagtaaa aagacgtctt caattcagat tggaaaaggt actggagcgt
    26701 attcatattc ttgaaggact gcttattgcc tatcttaata tagatgaagt cattgcaatc
    26761 atcagagaac atgagcaacc aaaacaagga ttaatttcac gctttaattt aaccgaacga
    26821 caggctgaag caatattgga aatgaaactg cggcatttag ccaaattgga agagattaaa
    26881 ttgcgtggtg aactggatga gctgtctgat gaacgtgata ctttacaaaa aacgctggct
    26941 agtcattctc tcttaaaaga actggtaaaa gaagaaataa cgaaagatcg agatgaattt
    27001 ggtgacctac gccgttcacc cattattaca agggaagact ctcaggcatt aaaagaagag
    27061 gatattttgc ctaatgagcc tattacggtt gtattgtcta aaaatggatg ggtcagagcg
    27121 gccaaggggc atgatgttga tggcaaggaa ctcagctata aagctggaga tgaattcaaa
    27181 gcccaaacgt tagctcgtac taatcagcaa gttttatttt ttgatggaga gggaaaggtc
    27241 tattcgcttc caggacatgc attgccatcg gcacgtggcc aaggcgagcc tttgaccggt
    27301 aaattaaacc ctgctgaagg agtattgttt gaagcaattg ttggtggaga gcctgagcaa
    27361 aaggtgttat tggctaccga ttccgggtat ggatttatcg ccaaaattga agatttatat
    27421 gtaaaaaatc gtaatggaaa ggcatgcatc aaattaagtg aaaacagtca tatattgccg
    27481 ccaaggctaa taccaaatcg agagcaatta tttgttgctt gtgcgactaa tgtagggcgt
    27541 ttactgattt tttctcttga ggagttgccc gaattatctc gaggaaaagg gaataaattg
    27601 atcagtatcc ctgttgctaa agcggtctca cgagaagagt atatagttga tatgcaagtt
    27661 ttaacaacga atgattcgct gacggttcat gccggtaagc ggcattttac tctgaaaggt
    27721 gaggatttga ctcactataa gggtgaaaga ggcaggagag gaaaccggtt gccaagaggc
    27781 ttacaaaatg tttctcaatt gcatgttact caatctgagt aacatggccg ttagagactg
    27841 tatgtttccc gtgaaacacc tttatgtgat gatttaaaat caaatagaat aatgcacaga
    27901 gctggcttat tgttcaataa ctgcaacgtt attctttctc aagagttttt cttaagattt
    27961 cttttctatc taatgataaa tctaacaaag gcagggtagc tagcgccatg cttttatata
    28021 atctcttgat ttcactatta tctatcgctt cgagatgcaa ggaaatggtt tgcatatctc
    28081 ctctagtcaa aggtccggtt aaagcacttt ttgctctatc tacctgattc aagttaagga
    28141 tattgctgta catgagatca catatcattt ttcttgattg tgcttgctgt attcccgctt
    28201 ttaaaagtaa ctcttcacta tatgaagcta aagtcactaa ataattagaa gcaatcgttg
    28261 ccgcagtatg ataaatgacc ttcatttcag gttgaattgc gataatgctg gcttccagct
    28321 tctcaaatgc aagcctaagc caacgacatg cctcataatc tccctctatc acgcaatgga
    28381 cttgcttaaa cgcattggaa tccaaagagt tagtgcggaa tgctttcaaa ggatgcgcgc
    28441 ttgcaatata acaaccctgt tttttcagag gaagaagtac acttgaatta agagctccgc
    28501 tgcagtggat caccaagctg ccttttttta atatctcaat attgtccaat atcgatacta
    28561 catccaaaat ggagtcgtcc ctacaggtta tccacgtaat atctgcttca gaaagatcag
    28621 atattttacc aactgcattg ccaaatccga tttcattgca ggctatcctg gcacttgcca
    28681 aatttgagtt acaaatagaa tttaaagagg caatctgatg aacagacatt gcaatgccta
    28741 aattcttacc cagacgccca gcaccaataa tattgaaatt catagtgaaa ccaatgtctg
    28801 tctcagtaca ttttcttatt ataatggaaa ataatttatt gtcttgctaa attatcagtc
    28861 tgaattaagc ggacattagc agagtctgag ggaatcgttt gcgataagaa gttgagtata
    28921 aattgtcatt ctagatataa aaaaataaaa ttatgtcgta atttgtgtct tctgattgtt
    28981 tttttctcga atttgtagca gcatgaagtt tccacaaaaa atcaggatga tattatgaaa
    29041 aaacggattg tttcattatt tgtcatttca acagttattt catctgtaaa cgctgcaacc
    29101 caagcaatcg tatggggtga gtcaacgaaa gcattgcctc aatttatgca agcacatcct
    29161 tttaagaaac aaggattatt gcaaactgta aaaagtcagc aaagtgatta caaactggag
    29221 ttacaaaata attctaacca tgcgaccagg catgctcgct atcaaataac ctacaagggg
    29281 gttccggttt ggggttatca agtcattttt cattccaaag atggcgccaa agaaactgta
    29341 acaggtatga atattaccgg aattgaacag gatattaatt ctactgaggg aaaattgagc
    29401 ccgaatgata tagaacaaaa gattcttgga aaggtaactg agccggttaa atttaaaaac
    29461 cttaaaaagg ttatttatat agataaagga aataaagctc atttggctta tcatttgtct
    29521 tactattcca atagtgaaaa aaaacatgtg aatgcaccta attatcttat tgatgccaat
    29581 agcggtgaga ttttgaaaca atgggatgag gtgcgtcatg aaagaattgg tcagggatta
    29641 ggcggtaatg cgtttactct cccataccgt cagggaatgt ttcagcatgg taatgctttg
    29701 ccggggcttc cctcattagg gaagtttgac gttaatgttg aggatggctt atgtcgtgtt
    29761 gaaaatgaat ccataaaagt aatgaatttg gaaaatcata atataggtta tgacttcttt
    29821 ccaattacta tatttgcaga gtctgtactg aatttaagtg ctttttccta tccatgcgat
    29881 gaaaccaact tgtttttaaa ctatgctgat ggcagaacag gtcctgtcaa ttatgctttt
    29941 tctccagtta acgatacgat gtattttccc aacaaacgtt agatatgtat caaaaagttt
    30001 atggtgttaa tcgtccaata ggtgatgatt tacctatacg ggcttacacc catcttggtg
    30061 atatggataa cgcttttgca gtaccaacca tcagtcttga tggggtagtt cttgcacatc
    30121 agcaaattgt aatcggaaat ggtgatgaat ttttaacagc tccagcccag agtgtgttgg
    30181 gacatgaatt atcacataat tttactgccc tgcattccgg attgatgtat gaagggcaat
    30241 ctggggggat caatgaatct ttctctgata tggcggcaat tgcattgtta gattatctta
    30301 gtaaagatta tccatggtat tgggatggtg aggattggac cattgggcgt gaagctgtaa
    30361 aaagtgggca acctattcgt tatttggatg atccagccaa ggatggaatg tctatagggc
    30421 atgctagtga atacactgat gcgttggatg tgcatataac gagcggtgta tttaataaag
    30481 cattttattt attagcacat aaaccaggct ggtctataca aaaagcattt caggttatgg
    30541 ttgacgccaa tatgaattat tggtctccta ttgcatacta tgattttgct gcatgtggcg
    30601 tcattcaggc aaccatagat aagcattggg ataaaacacc tgttatcgag gcatttgccg
    30661 aggtgggagt cgtttgtccg atgcataaaa gctagtttta agttgtttga cttcagttca
    30721 agctacttga aacaacgaga gttgctaagg aagattgatg attttctata gaacgcctac
    30781 tggataagat aattattcca ggtattcagc gcttaaaaga cgaggcttag ttcaatagac
    30841 ttcttgccta accagtctct ttcgttttat tgcagcgttg caaacgtttc tgcggcgctc
    30901 atttgctgta cgtaatcttc gttccttaaa atgtttgcgc caaaataatt aggttatgca
    30961 agaggcttaa taaagactgg cttggagcca ctagaggata ggtagcaagg gctaagccac
    31021 atccgagtca aggtttctct acgttgagga gcttattcct caaaaacaga gagggaataa
    31081 aactcaattt atttttaaaa aaaatcccca
//
LOCUS       DATNSN010000022        30698 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.022, whole genome shotgun sequence.
ACCESSION   DATNSN010000022 DATNSN010000000
VERSION     DATNSN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30698
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.022"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..484
                     /locus_tag="VAH76_000800"
     CDS             <1..484
                     /locus_tag="VAH76_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HEO1385447.1"
                     /translation="GKVEFKFYKHDGVHVLERKTSQGWFRLMQTDLLPIDLKTVEMNL
                     LKLERYPDTISIRDNKTVVGSITEYGRKSLVWEAQSNQLKFSKHDGDEVIQKTLTSFE
                     EGVQILVKEFDIDHISAQELKIYCTETNLPQPIKPWTVDFPLDQAELKKMEENLIFRL
                     "
     gene            complement(564..1292)
                     /locus_tag="VAH76_000801"
     CDS             complement(564..1292)
                     /locus_tag="VAH76_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385448.1"
                     /translation="MTPSISLITSYSLLPAILMLIGGGIASLYRPGGSITSATQHFAA
                     GVVFAAVAKELLPKIGAYHDPVALIIGFSVGILGMLFLKWLANRLEDLEQEKTGLSWG
                     LLTAVGIDLLIDGILIGVAFLAGERGGILIAIALAIEIFFLGLSTTATLGARQVSVPI
                     CLLTSLILAILIPVGAALGASLLIHLPLSITNGILSFGVAALLYLVTEELLLEAHEVR
                     ETPYITLCFFIGFLLILLLEGLAA"
     gene            complement(1375..2055)
                     /locus_tag="VAH76_000802"
     CDS             complement(1375..2055)
                     /locus_tag="VAH76_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEO1385449.1"
                     /translation="MKPIAHIADKEIISIPVKECGEELIDLKNQNIIVYGPPPETPLT
                     ANDYTKIRKTVYQKLCLAQNDLPNGWRFRLYEGLRSLTVQEILFNQQRDNLRQSKQYD
                     SYEDLFNETCRLVSPVVHLDGTANIPPHSTGAAIDIELIDSYGNLVNLGMAAKDWLIV
                     DPDLSETYSKLISEEAMQNRILMLKIMAKHGFVNYPREWWHFSYGDRLWAYMTEHSTA
                     IYGAIINN"
     gene            complement(2239..2517)
                     /locus_tag="VAH76_000803"
     CDS             complement(2239..2517)
                     /locus_tag="VAH76_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385450.1"
                     /translation="MSISMITEIETLLKKAGFTYVGNGRGLGEFEKYENHLYHKNVYG
                     SIQQIQLAMDRENDTVRPVFSKNVPIELRDSIYELMKNPSEKNSLQFN"
     gene            complement(2602..3087)
                     /locus_tag="VAH76_000804"
     CDS             complement(2602..3087)
                     /locus_tag="VAH76_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385451.1"
                     /translation="MNRLTSFLLLLLITCSSGSPYLYAANKNNDFVLFEKIFTDWTLA
                     FNQKKLAPTCALFSKSLIANYQGAPQKNYTSICHGFKKIFQQTRKNYQYHFKLKQVYR
                     SGHLAVARITWYLQIFEQGKLISETQDEGLDVFLKDARNQWKIINFIGYPVPGNLDEK
                     H"
     gene            3234..3848
                     /gene="mavI"
                     /locus_tag="VAH76_000805"
     CDS             3234..3848
                     /gene="mavI"
                     /locus_tag="VAH76_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavI"
                     /protein_id="HEO1385452.1"
                     /translation="MSSVKLCFALFRLYCYWRKLSMGLSAQQKTAIKSYFESNYNLFF
                     GIPTERKEGDLTPNEHVMVLQYLRFYLLKLPIECLEEELVNKQINGDDTVGILLEDFL
                     GNSLSPMNPDAYFHSQKNFIQMGNAEDPAKPEDIIQYLIEVIENMSQQMDHGYLKKES
                     DPKIEKSNSSYASKALKWGLFGGAALAITYAVSKLSDKSHTNSL"
     gene            4093..4626
                     /locus_tag="VAH76_000806"
     CDS             4093..4626
                     /locus_tag="VAH76_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2443 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385453.1"
                     /translation="MIRHFFIVLFFFVGFMNQVSALPWFENDLRNNNKLRYRAHDEIK
                     KNQIDFSGRWSGQCDSNPAVDLTIKHTREHISISYGFMEEKYIPGEVRAESSARLSLS
                     ESGSTTVKWNQDNTALIFINYHLFINESGSLNVFFSKVSMALEQEQLVVKGQHFETGN
                     TLDDIKQETISCIYDRK"
     gene            complement(4746..5156)
                     /locus_tag="VAH76_000807"
     CDS             complement(4746..5156)
                     /locus_tag="VAH76_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1385454.1"
                     /translation="MKPLIPYLLFNGTCREAMTFYQNCFGGELELMTYGDSPEGACDK
                     KADKNEIMHACLRQGSFTLMASDWPGGEAKQGNNVHLNIDCSTKAEIEALFKKLSQEG
                     TVVQALADTFWGAHFGMLIDKYNIHWMLNYSLKQ"
     gene            complement(5257..5730)
                     /locus_tag="VAH76_000808"
     CDS             complement(5257..5730)
                     /locus_tag="VAH76_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1385455.1"
                     /translation="MQKITPCLWFNDKAEEAVTYYISIFKNGSIEKITYYGKEGVEFH
                     KKPAGSVMTILFNLNGQQYVALNGSPEFRFNESISLMVNCDTQDELDYFWKELSADGE
                     EGPCGWLKDKFGLSWQIVPKILAELLSDHQKSEKVMRVLLQMKKLDIAKLKAAAE"
     gene            complement(5768..6727)
                     /locus_tag="VAH76_000809"
     CDS             complement(5768..6727)
                     /locus_tag="VAH76_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO1385456.1"
                     /translation="MGILIDGIWHDENNINTDKEGRFVRAESGFRQHITASGESGFKA
                     EPDRYHLYLSYACPWACRTLIMRKLKGLEKIISVSFSDPVMGKNGWSYGESGNETADE
                     INHIHYLKELYLVARKDYTGRVTVPVLWDKKKKTIVNNESSEIMRMLNSEFNAFSSTD
                     HDYYPKQWRQKIDEINQLVYENINNGVYKCGFAKSQKAYEEAFDALFNTLNQIENLLA
                     TQRYLIGEQLTEADWRLFTTLIRFDAVYHGHFKCNLQRIADYPHLSNYLRELYQFPGI
                     KETVNFNHIKQHYYRSHLSINPTGIVPKGPKLNLDEPHNRYEF"
     gene            6876..7427
                     /locus_tag="VAH76_000810"
     CDS             6876..7427
                     /locus_tag="VAH76_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO1385457.1"
                     /translation="MFNVAVIVGSLRKESFNRKLAFALDKLQHPTFQLSHIKIDDIPL
                     YNQDFDNNLPDSVIRLKKEITEADAVLFVTPEYNRSIPGVLKNIIDWGTRPYGKNVWA
                     GKLMAAIGTSPGAIGTAVAQSHLRSIMVAIDGVYFGQPEIYLVYKDGLIDNNFSITME
                     STRNFLQGFLDRFAMEIEKHKSV"
     gene            complement(7471..7995)
                     /locus_tag="VAH76_000811"
     CDS             complement(7471..7995)
                     /locus_tag="VAH76_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385458.1"
                     /translation="METEEDRVKHTNIKRFKELHHQKERQLQEEEEYNRLFNQLRDEI
                     IAHPKSLKEEVELNYIFSEGAKPELIELLKDYKNEFGKEPMPHQGGFALSFNSKEEAV
                     DFFKSQSEKGRAFDMYNARIDHRVYSDGKGVFVHGTNAEVMDYLKNKEKYDVGKDGAL
                     AKIDNREHGMSMQK"
     gene            8228..9418
                     /locus_tag="VAH76_000812"
     CDS             8228..9418
                     /locus_tag="VAH76_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEO1385459.1"
                     /translation="MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRFFKTDS
                     TTMQATLYLFMLTVALGQLLVGPLADHFGRRQMALSMALLFFAGSLISALAPSVPALI
                     FARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDV
                     TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVYQVMRQIISNSDFRQYT
                     LVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMAVFVANMMAARLTYSHS
                     LESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDF
                     EQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRI
                     LSGS"
     gene            complement(9777..10157)
                     /locus_tag="VAH76_000813"
     CDS             complement(9777..10157)
                     /locus_tag="VAH76_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385460.1"
                     /translation="MTRNSVKSVCIFLSIILYVTALCLPAAIIPDFERGIHQTHYGFE
                     ILITGWMGFLAWQPAWLANLLYLFSLLTYGSKSSYRFGTAAFLLALVSPFILVEKLLI
                     GFYVWLVSFAVLLYGNNLHEMTTE"
     gene            complement(10194..11237)
                     /locus_tag="VAH76_000814"
     CDS             complement(10194..11237)
                     /locus_tag="VAH76_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEO1385461.1"
                     /translation="MITNNIRFNWIIFILLIGLLNLANINELYADETGEQNQFNLESY
                     SPQTKKLCQTVAHISVPSQNQLDEASKASLKNCDALDLYYGFNAAPDYDKAFQCALMN
                     KDYNVLVMAYANGRGVTFNPDLAMHFACMMEDAAPAEMDGRIAHLAQIKEGNKKTTFD
                     ICDDITSGYMMGWCSSIDQRLEDVKRNQKIKALVSQWTAQEQLLYQQVRKTAEVYIRD
                     HSMNEIDLSGTARSAFAINAQLNLNQRLFELLQKVNRCETPLMTIKQYEEMDKQLNSI
                     YKKLMADTSSFQYTTVTKEGIKKTEIAWIKYRDAWIQLARVKCPKISAESWQVLLIQD
                     RIKLLNEILELAE"
     gene            complement(11264..11758)
                     /locus_tag="VAH76_000815"
     CDS             complement(11264..11758)
                     /locus_tag="VAH76_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2434 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385462.1"
                     /translation="MTIMMNKDKHSLLAIAAAEVPPRTKPSIYPEPFASMMTGRQKHA
                     LGDFFGIKNFGVNLTRLAPGAQSALLHKHKLQEEFVFILKGQPTLVTETADIQLHPGM
                     CAGFTPDGTAHQLVNRTADEVVYLEIGDRTQGDEVNYPADDLAAAFGNDGQWHFMHKN
                     GMPY"
     gene            complement(12025..13821)
                     /locus_tag="VAH76_000816"
     CDS             complement(12025..13821)
                     /locus_tag="VAH76_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385463.1"
                     /translation="MEIYMAPLFPEKNAPLTIKQGPVDDCYLLVALDCIFNSGDEARQ
                     LLASKFTQTPLGLTLRIKRNNQSTRLDSQKMRGKYIHVYDRNTDEDVFFIGQQKLKEI
                     DNSKKGVETNSLAIKVLERISSYYYTGDWDHTNESGSLTAHSIRQRHMETSTRFVGKL
                     LGIHAEDTEEMDAIIKLKLMNPKEAVYISFAYGEPDANGKIHDRHALRIDKVVPKING
                     GYDFILVNPWDNQKQETFSLEELEKRDCRFCTFSLNQQRYEITRILLKNPIGQGQYIF
                     ANPELFTLLLQMQKLSLLSDAKNIQACINLHKQMPYLPSLYNSLTSDEQKKLIQGMVG
                     TKGDKEKFIKHLIINAPRLDLIKTIFQNEPPENKTENMIVDLALEAKKKNSPARIVFE
                     DPEFFTLIMNRAIHHKATIRGCSQEQAKKLIESKMINFYFDNLGTDSLTRNARLCELF
                     AAKIFTKTSIETWFAARLSLTNVDLSQALRTLGFSDQRSGVMSAPVLKNQTALVARQV
                     GFFPIHKSSPAPVANKSPAPTQELTAKKSSVPTLPSIVKKPSTPLSEPIGKKLSTPIP
                     ALLVNGLSIYTPMKPSVKTLPPLKGVRLTSLP"
     gene            complement(13987..14880)
                     /locus_tag="VAH76_000817"
     CDS             complement(13987..14880)
                     /locus_tag="VAH76_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO1385464.1"
                     /translation="MKNLLFLVLVILTNSLMAKENLVFAVIGDYGANSAAEAKVASVL
                     KSKNPQFILTLGDNNYTHGCWKTIDKNVGKYYHEYIGNYQGKYGKGSDVNRFFPTLGN
                     HDWLARKACLYQGTLPYFSYFTLPGNQSYYDFVRGPIHFFALDSDSHEPDGSKEGSKQ
                     YQWLTEQVQQSKAPFKIVYFHHAPLSSGKHGSNTRMQWNFASMGIDVVMSGHDHHYER
                     IERNGIVYFVNGVGGAELYSYKKWVEGSKFFYSKHHGFMLITALEHAMKIQFINENDE
                     IKDEIVIQEKKPGAGEHQNLR"
     gene            complement(15014..15961)
                     /gene="mdcH"
                     /locus_tag="VAH76_000818"
     CDS             complement(15014..15961)
                     /gene="mdcH"
                     /locus_tag="VAH76_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HEO1385465.1"
                     /translation="MKILFLFAGQGYHADSLFDIFRTDKKTINYLKTMSSAAEIDFLQ
                     NNLEMTNPYSIQSIIGAYQLTLFSRLLPLFTNHQVDLAGYSLGEISAFLGSINANPRD
                     SFKLFRYRTQLMTSIVDNNIFAEYDLLSVVGKFILEEIQPVIAKHNCYIAIINSIQHV
                     VIGGKISDLRHLITELSQYPISHAKFLGVYLPSHTDFYSDKSKQFQRFLDENYGFYSL
                     KYPVISPLELNKIYNAKQERALLAQELYTTLQWHKVCALIGEYQYNLIVDLGPGDSMT
                     TILNKANTELFNTPLITASHYNSLKGLCNAITSLSSSYQ"
     gene            complement(15958..16800)
                     /gene="mdcB"
                     /locus_tag="VAH76_000819"
     CDS             complement(15958..16800)
                     /gene="mdcB"
                     /locus_tag="VAH76_000819"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEO1385466.1"
                     /translation="MQTFQHSLPNPYKIARYFAKKAVQALYDEVALYPKPGLVSFIDS
                     GAHQDMNGALFFRSLFGLRHYFFQIGLHTALGSSPKKLVTFGLNAEKTMLKITGGVNT
                     HRGAIFALGILCTSICKLSAKYHAFSTEDLHYAVIEQWAQYLEKEHQNRDTHGTLVTK
                     RYAVPDAKQTAIQGYKAVFHTYNELSNIIHDQTFFGLLAYQRLLLAIDDINILYRTGP
                     EGLEFARYHVQQDICQNNREKSIQKAIELHQLFSKKNISPGGVADMLGLIYFLRHVFS
                     REAK"
     gene            complement(16772..17395)
                     /gene="mdcG"
                     /locus_tag="VAH76_000820"
     CDS             complement(16772..17395)
                     /gene="mdcG"
                     /locus_tag="VAH76_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HEO1385467.1"
                     /translation="MNYPRHTLCYLDKNTTTAILPAPEQLDILQYWLKCEYPLIVTRQ
                     PEDVPCGQIQLAIPYFIPTQSQKLRASYVVDDSWIIDTKALPSLQEIFPYLELDTSAK
                     IQVYGSYCWQHLTKEQYIQSSSDLDVLISYENQSLLTLSELHKHLQKMLKIDCIDGEI
                     RFPALGDCSWAELLQTHSSDSILFKAAKKIFLVKRESLYANFPTLLA"
     gene            complement(17392..18108)
                     /gene="mdcE"
                     /locus_tag="VAH76_000821"
     CDS             complement(17392..18108)
                     /gene="mdcE"
                     /locus_tag="VAH76_000821"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HEO1385468.1"
                     /translation="MPNEKITLKQLIDHCFDTTKTTIKQQIVFGSGKIKNKNFNIVGT
                     VEDTSFGVDESIEMARCILDIIKNNNTDPIVLLTDVVGQKLSVRDEWLGMYAYFAHLL
                     KCLHLARQNGNKIITLIYNQAIGGSFIAFGMMADRIYALKNAKLAVMWLEGMSKVTKI
                     DIDVLRKISETSPVFAPGVENFKKLGGLHQVLDLDEVSQQLLLTLQEPNISHDNRAEL
                     GKQYGGRKEAYDIIKAIENL"
     gene            complement(18112..19308)
                     /gene="mdcD"
                     /locus_tag="VAH76_000822"
     CDS             complement(18112..19308)
                     /gene="mdcD"
                     /locus_tag="VAH76_000822"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEO1385469.1"
                     /translation="MDKYHYTFSLPKKIQISPMIKVGVAGSGNLEVIIKPNSDFDKTE
                     IIVNTVISGFRNTWDAVIERFVEDYPYSGLSITLNDAGATPPVVSLRLRQAMETYQTG
                     YPKKDSYTEANARNRIYSLVDEASFTEFLLDKETPSPTLPQLNMQVETDDGVIIGTAK
                     MDGIDIAIASQQKDFIGGSVGEIHGAKINGLIKYAIKNQLPAIIFLIDSGGVRLQEAN
                     VGEIEISEIIRSILDARSAGIKTIGVICGNNGAFGGMGIISGTLDYLIVNQGARIGVS
                     GAEVIQAVKGVEVFDSSNRPLVWRVYGGRTRFLKADVQGYTTNKTMDIRQAIKTALKT
                     LPTAPSLNLNSILAEHEQLQKRIASAKNCREEGEWLKNNRTELYQQDIFNVSDQQFLA
                     LTNKGK"
     gene            complement(19317..20963)
                     /gene="mdcA"
                     /locus_tag="VAH76_000823"
     CDS             complement(19317..20963)
                     /gene="mdcA"
                     /locus_tag="VAH76_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEO1385470.1"
                     /translation="MDWNKNLKDYESRLINIKPFFKKGKIVSEKNIVALLETAIKPHD
                     KVCLEGDNQKQADFLARSLCSVNPQKIYGLHMLQSAVTLSEHLDLFERGIAEKIDFAF
                     AGPQSTRLAIMIQRNQVKIGNIHTYNELYARYVMDLTPNVALITAEKADYYGNLYTGA
                     NTEETPSIVEATAFKNGLVIVQANEIVKKLPRIDIPGEWVDAIVQAPSPSYIEPLFTR
                     DPALIDEVKILMAMMVIKGIYAPYQVNRLNHGVGFNTCAIELILPTYAHSLGLKGKIC
                     KHWMVNPLPTLIPAIEAGFIESIFCVGGEVGMNEFIRSKPDIFFTGRDGSLRSNRLYG
                     QLAGHYGCDVFIGATLQMDPQANSSTATKGRIAGFGGAPNMGCDTLGRRHSSYAWLKA
                     GQEKNSHNPVAMPRGRKLVIQMVETFQGAGKPTFVEKLDAWELQKEMQADLPPIMIYG
                     DDISHVVTEEGIANLLLCKNSEEREQAIRGIAGYTPVGLKRDKAIVDELRHRGIIQRP
                     EDLNISLKEADRDLLAAKNIHDLVEISHGLYCPPNKFRNW"
     gene            21243..22049
                     /locus_tag="VAH76_000824"
     CDS             21243..22049
                     /locus_tag="VAH76_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385471.1"
                     /translation="MDMREKYTGRSEPIINKPSDIPKGLVAKRKAELLLAKLEEAIKA
                     QESLNRESESPAKEGNSESLKPRLTHPTSERPKINHRPPSSFSYTNSDSTLFTSVKEE
                     KTEGEVSSVSLSNDKKKVEDNSSASIWTELIAALSRAVDNYTKKKMEGTTPGEAYGWF
                     SWLRHGKAGEDKAGGLASEARTTSTVESLLTLLHHFFADSTTRYNNHSLAPYVMDELN
                     KLLIQYALKPFILSLGSQYDLNSWLHIKNQLTQLAGKEACQEKQSLVAPV"
     gene            22240..22794
                     /locus_tag="VAH76_000825"
     CDS             22240..22794
                     /locus_tag="VAH76_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385472.1"
                     /translation="MNTKGILCAVLLLVGTVLAEIGYAEFNENGNSIVATNNSQNTSE
                     QSSQQNPKRSPSGQNCTGTSPNPFPQNPASSPFSIGQLGACVNSTACNATNATLKSKI
                     ADRYCQDANKATCDANDDCLPPPDCKGRVNGTASSIVLANCQNAGPTLCTSTGGGIYC
                     LCDAEIAANAQIKCGCECTTKTLP"
     gene            23120..24463
                     /locus_tag="VAH76_000826"
     CDS             23120..24463
                     /locus_tag="VAH76_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385473.1"
                     /translation="MKEIIELLATLDELQKPYIHRVKSRLSELLNLDEKENELMRIEQ
                     STSRSWEEGATTDLRIGPIKSKKRIEEIRAVLITLSGASAVKYEEGHNLENNTVEFII
                     TACDVEKLEGFIPPNQEEMQNTIPFTTFIRMSIAGNRGYVRGISFDSAKLDGESYLGS
                     FKEGELTTSKQEWFSVLELLDLLNRESELNLIQIQNDLKSLLGVKFEVYFHSSSEEFP
                     TDLRTGSLDLKETGAQQLLRSMDSLVLLDCLKTENKELEDGQKVCIIKQVNPQELHRF
                     SLKKGKNTNANAEMLSNIIELLKRSFPNNQVIQDLVVFDGRDGRSMSRPYFHIRTQNY
                     HQEGTSDYKSAMSILKKLQDKTSKKIIFDYRNEMVEMKDESRPIYMSSIENTIKSSQF
                     IIDVAVGLTTEQLPELQNICAEINQSRLKHHRHNLFKPQIEEQKINEQCRVLFIG"
     gene            complement(24552..26246)
                     /locus_tag="VAH76_000827"
     CDS             complement(24552..26246)
                     /locus_tag="VAH76_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385474.1"
                     /translation="MKIILSILIALHATSTYAGQQVLVLSGGDNPGLNHYTQYLQTKT
                     LYEHLITRFNPETVSIYFGGGNTPTTQPSPFDVHKLINTKDNQQNKEDVMFTGIIANN
                     QQANKYNVDAFFLSSKIQQINPGDNLLLFVSDHGMPHGFLEDKNTNPYSNNCIDLWHY
                     QKPFINNFTNAKNFSKACLSKNELSSLLALIPSQHVIFAMSQCYSGGFHQLSVTTQDG
                     YPVANTKICGFTSSTDDHYASGCTADANGATYQGYERSFTEWYTGHGVIDGKKIREPA
                     KSMLVAHQNAIIEDMTVDIPLSTSDYYLLQWAKLFASKQFKSRSKHYDNNMIQSIYLN
                     YKKRLQSSNNDALYQFMHIARASEDKIVQLMPEAREFASLSLTKQKQKISSLEKQLEQ
                     QGKELQAVWEGMMNLTLNVIMPSWEKAVNQSKSQPLNEKQQTFEKEFYQTIVKLNLYQ
                     HPYQFEMYYLQYLSEKQNDPDLVDYQKNRNAIIYQWARAHNKDPLANAIKSWEKLNKK
                     QLSLTEHIAEEEKKKQWLKRIFTYNQIIASWVTLLTIHDEKALADLEGLLKCQNAS"
     gene            26572..28836
                     /locus_tag="VAH76_000828"
     CDS             26572..28836
                     /locus_tag="VAH76_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5621 domain-containing protein"
                     /protein_id="HEO1385475.1"
                     /translation="MSTSEKDVREQQVKTVTLSFLGTGQHRENVHHILTSFHNTISEV
                     HKDNPTVATRMFDGPGSEPKSSGSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQK
                     LTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPETVNLHGFSRGADTCMRMANLLYQL
                     YPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVI
                     ADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLHDDMNRFCRETGSLPSVGISPP
                     IIARVGDKKEEVRTHSELSPEKRFELLCGMKENESEYAKLKKKHHERSILSKQEDYVQ
                     DSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTQKEEVIAELKSLSEDPRYEHFFSS
                     LAKHFQINENNVAGTLPEPSGVDRDEKSSYRQPAVRDRLSYLQHSLTSIANYYHYHCD
                     EKNSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQTLESKNEKGFLWQQINH
                     ISPNTQQYCEQVKAVLQEHLEHNQVLSDTQKEEIRKAMDRMDNIVNDGSKDSQQKYRE
                     IRREVIELNAKATTPEDESQYLRNQFHKAYFRLSGDPHKNLSLNSLSKTLNQLSEAHY
                     GETNMTDKIIQRLDSYKNRNSFWNSVKEVLNFFNIPFPKLHSEVKEQIADALQKRLVD
                     LNEKGMGNDVNTITRELGKAREDLIEHYKKTSKLEMGELDKIINKSMEELLVARKITT
                     KDLVHEEVSQVKLN"
     gene            complement(28934..29485)
                     /locus_tag="VAH76_000829"
     CDS             complement(28934..29485)
                     /locus_tag="VAH76_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385476.1"
                     /translation="MKKDITLFTSARPELKFVRLSECKEHLDTAAMWAKNAWGYMHLD
                     DEQFHYKQLQELCEHVYIAMYGGLPVGMLAVIEKKLSFEENREKSSEKIVEEPSTPET
                     TEMKNAYLDYVYIDERARSMGLAGIIIEKAKSLAREMNAEVISLETLNSTLNGLYKRK
                     GGRVICEGRYNDSYPTEVIRLKF"
     gene            complement(29690..30673)
                     /locus_tag="VAH76_000830"
     CDS             complement(29690..30673)
                     /locus_tag="VAH76_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385477.1"
                     /translation="MTLSYKEAIHIFLPKTKNKEQLRQLLCLYYAHFDRNDKQFWCDV
                     HKKGIKSEVICCAAAITGCSSALDTISLSLMPDEIVKMIQAENYHAFRLAAENGHLHV
                     LNRLCELAPTEVMAMIQAENYHAFRLAAENGHLHVLNRLCELAPTEATAMIQSENYYA
                     FRWAAVGRGHHNVINFLLDCPAMLGYAEMHEFEYGEKYVNPFIARHVNRLKEMHDAFK
                     QSNPEDLFDLVTKSECLQGFYMLRNLIRRNDEALLDDIRFLLSIPGIKALAPAGTIPG
                     NENELLRLALRLGNQGACALLLSIPSVLALTKANNYYIDETGGRLDLRAVA"
BASE COUNT         9193 a         5635 c         5984 g         9886 t
ORIGIN      
        1 aggtaaagta gagtttaaat tttataaaca tgatggtgtg catgttctgg agagaaaaac
       61 aagtcaaggg tggtttcgct tgatgcagac tgatttgctc cctatagatc tcaaaacggt
      121 agagatgaac ttactcaaat tggaacgtta tccggataca atttctattc gagacaataa
      181 aacggttgtt ggatctatta cggaatatgg acgtaaatcg ttggtttggg aagctcaatc
      241 aaatcaattg aaattttcaa aacatgatgg tgatgaagtc attcaaaaaa ctttaaccag
      301 ttttgaggag ggagtgcaaa tacttgttaa ggagtttgat atcgatcata tttcagcgca
      361 agagttgaaa atttactgta ctgaaacaaa tcttccccag ccaataaaac cttggactgt
      421 ggacttcccc cttgatcaag cggagttaaa aaaaatggag gaaaatttaa tttttagact
      481 atagaaaagt gatttgtatt ggctattcca ggcaggttca gcccatcaga acttatcttt
      541 gttattaaaa ggcctgcttg tcgctatgcg gccagccctt ctaacaataa aatcagcaga
      601 aacccaatga aaaaacataa agtaatataa ggggtttcac gcacttcatg ggcttcaagc
      661 aataactcct cggtgaccag ataaagtaat gccgcaacac caaatgataa aattccattg
      721 gtaatcgata gcggtaaatg aattaataaa ctggcgccca gagcagctcc cacggggatc
      781 aatatagcca aaatgaggct ggttaacaga caaattggta cgctcacctg ccttgctcct
      841 aaagtggcag ttgtcgataa tcccaaaaag aaaatttcaa tggcaagggc aatagcgata
      901 agaattcctc ctcgttcacc agccaaaaaa gcaaccccaa ttaaaatacc gtctattaat
      961 aaatcaattc caactgcggt taataatccc caggatagac ctgttttttc ttgttcaaga
     1021 tcttcgagcc tgttagccaa ccattttagg aataacatgc ccagaatgcc tactgaaaag
     1081 ccgataataa gagcaactgg atcatggtat gctccgattt tgggtagcag ttcttttgct
     1141 acagcagcaa aaacaacacc tgctgcaaaa tgttgggtgg cgctggttat tgaaccacct
     1201 ggacggtaaa ggctcgcgat gcccccaccg attaacatca atatggcggg taataaagaa
     1261 taggaggtaa taagcgaaat agatggggtc ataagagata ttctttaagg taatattgac
     1321 agaataatca aataattagc ccagttcaag aatggcaggc attgccacat tggcctaatt
     1381 atttatgata gcaccgtaaa tggcagtaga atgttctgtc atataggccc ataggcgatc
     1441 tccataggaa aaatgccacc attctctggg gtaattaaca aaaccatgct tagccattat
     1501 tttcagcatc agaatacgat tttgcatagc ttcttcagaa ataagttttg aataggtttc
     1561 acttaaatca ggatcaacaa ttaaccagtc ctttgcagcc attccaagat taaccagatt
     1621 gccatagcta tcaatgagct caatatcgat tgcggccccg gtgctatgtg gtggtatatt
     1681 agctgtccca tccaaatgga ctactggaga aaccagacga caggtttcat taaataaatc
     1741 ctcataggaa tcgtactgtt tgctttgacg caggttatca cgttgttggt taaacagaat
     1801 ttcctgaaca gtcagactac gcaatccttc atataatcta aagcgccagc cattgggtag
     1861 atcattttgt gccaagcata atttttgata tacagtttta cgtattttgg tatagtcatt
     1921 tgcagttaaa ggggtttctg gagggggccc ataaacaata atattttggt ttttaagatc
     1981 aatgagctct tcgccgcatt ctttaacagg gatagatatt atttctttat ctgcgatatg
     2041 tgctattggt ttcattattt tttcgcaaaa caagcaaaat tttattgcat ggattcgaca
     2101 aattatctgc acattaatta tatcatgcta acaaaaaagc aggttaggcc ttatgcttta
     2161 attttttaat atttttctga taaaacctaa caatctgcag ttggccattt aaggaaattt
     2221 ttggctcaaa ctctaatgtc aattaaattg taacgaattt ttttcagatg ggtttttcat
     2281 gagttcataa atactgtcac gtagctctat tggaacattt tttgagaaca ctggtctcac
     2341 cgtgtcattc tctctgtcca tagccaattg gatctgttgt attgagccat aaacattttt
     2401 atggtagaga tgattttcat atttttcaaa ttcccccaag cctctgccat tccctacata
     2461 agtaaatccc gcctttttta gtaatgtttc aatttcagta atcatgctaa tactcatcaa
     2521 ataaataggg tggcgattat attgataaaa tacaacaaga tcaatcaagg agcatattac
     2581 aatttattat gccaccttgt tttagtgttt ttcatcgaga ttaccaggga cagggtatcc
     2641 tatgaaattg ataattttcc attgatttcg cgcatctttt agaaagacat ctaatccttc
     2701 atcttgcgtc tcggaaatga gttttccttg ctcaaatatt tgcaagtacc aggtaattct
     2761 tgcaaccgcg agatggcctg agcgatagac ttgttttaat ttaaaatgat attggtaatt
     2821 tttccttgtt tgctgaaata tttttttaaa gccatgacaa atagacgtgt aatttttttg
     2881 cggagcaccc tgataattgg caattaatga ttttgaaaat aaagcacagg ttggtgccag
     2941 ttttttttga ttaaatgcca aagtccagtc agtaaatatt ttttcaaata agacaaaatc
     3001 attattttta ttggctgcat aaagataggg agagcctgaa gaacaggtta tcaagagaag
     3061 caacaaaaaa ctagtgaggc gattcatatt gattcgtgaa acccaaagaa aagattaact
     3121 taacaaattc ctccaaacaa tgacagcaaa ttcttaatgt tgtttttatt tgattattta
     3181 taatttggac ataaaattca ttgtaaggtc atcatgaaag atttaagatc actctgtctt
     3241 ctgttaagct ttgctttgca ttatttagac tttactgcta ttggaggaag ctcagtatgg
     3301 ggttatcagc ccagcaaaaa acggccataa aatcttactt tgaatccaat tataatctgt
     3361 tttttgggat tccaactgaa cgtaaagaag gcgatttaac acccaatgaa catgtgatgg
     3421 tattgcaata cctgcgattt tatttattaa agttgcctat tgagtgtttg gaagaagagt
     3481 tggtgaacaa acaaatcaat ggtgatgata cggtaggtat tttattagag gattttctgg
     3541 gcaactctct ttctccaatg aacccggatg cttattttca tagtcaaaaa aattttatcc
     3601 agatgggtaa tgctgaagat cctgcaaagc cagaagatat tattcagtat ttaatagaag
     3661 tcatcgagaa tatgtcgcaa caaatggatc atggttattt aaaaaaagaa agtgatccca
     3721 agatagaaaa gagcaacagt tcttatgcgt cgaaagcact aaagtggggc ctatttggag
     3781 gagctgcttt agctataaca tatgctgtca gcaaattgtc tgataaaagc cataccaata
     3841 gtctgtgaaa gcgacaatat tcggtttcgg ggtaacggaa tattaatttt cagcagattt
     3901 ctcgaaatac gtcttatacc aaagcaaaac catgctatgt tgtaagagtt gtgtcaaaag
     3961 caaggaacct gaattactat ttccctggat actccttaaa aagtagattg caatgatatt
     4021 ataaattcgt tttctgcgag acagaaaaag tattattctg tacaaacctg cctacagata
     4081 aggaagtcag aaatgataag acattttttt atagttttat tctttttcgt cggttttatg
     4141 aatcaggttt cagctcttcc ctggtttgag aatgatttaa gaaataataa caaactgaga
     4201 tatagggctc atgatgaaat caaaaaaaat caaatagatt tttctggcag gtggagcggg
     4261 caatgtgata gtaatccggc tgtggaccta actatcaagc atactcgaga acacatttct
     4321 atttcctatg gatttatgga agaaaaatat attccgggtg aggttcgagc agagtcatcc
     4381 gctcgcttat ctttatctga aagcggcagc accacagtga aatggaatca agataacaca
     4441 gctttaattt ttattaatta tcatttgttt atcaatgaaa gcggtagttt gaatgtgttt
     4501 tttagtaagg tttccatggc attggagcaa gagcaactgg tagtcaaagg gcagcatttt
     4561 gaaacaggaa ataccttgga tgatattaaa caagaaacca tctcatgtat ttatgaccgt
     4621 aaataatgac atgaagtaaa tccctgtatg atgagctttt tgttggtcat atgggattta
     4681 ctgggtcgtg aaacaacatc aatcgattaa caattgtcga tcggactttt tttgtatttt
     4741 gatttctatt gtttcaaaga ataatttaac atccagtgta tattatactt atcaatcagc
     4801 ataccaaagt gcgcacccca aaatgtatcg gcaagtgctt gcactaccgt tccttcctga
     4861 ctgagttttt taaatagagc ttcaatttct gcttttgtgc tgcaatcaat gttgagatgc
     4921 acattattgc cttgctttgc ctcgccacct ggccaatcag atgccatcag agtgaaacta
     4981 ccttgtctta aacaagcatg catgatttcg tttttatcgg cttttttatc acaagcccct
     5041 tcaggggaat caccatacgt cattagctct aattctcctc caaaacaatt ctgataaaat
     5101 gtcattgctt ctcgacaggt accgttgaaa agaagataag gtattagtgg tttcatctat
     5161 tctccttgat aaattgatta ttgcaagact cgtaactccc aatttctttg ctaaaggtcg
     5221 tttattgctt cgattttgtg atttttcctt agcatcttat tctgctgcag ctttcagctt
     5281 tgcgatatcc agttttttca tttgcagaag cactctcatg actttttctg acttttgatg
     5341 gtcactcagc aattcagcca ggattttggg aactatctgc caagataacc caaatttgtc
     5401 tttgagccag ccacaaggac cttcttctcc atccgctgag agttccttcc agaagtaatc
     5461 caactcgtct tgcgtatcgc aattgaccat cagtgagatg gattcgttaa atcgaaattc
     5521 agggctgcca ttaagtgcca cgtattgttg tccattcaaa ttgaagagga ttgtcatcac
     5581 actacctgct ggttttttat ggaattcaac tccttctttg ccatagtaag taattttttc
     5641 aatactccca tttttgaaaa tcgagatata atatgtcact gcttcttcgg ctttatcatt
     5701 gaaccataga caaggagtga ttttttgcat ggttttttat cctaatttct agctattgaa
     5761 aaatttatta gaattcatag cggttatgtg gttcatccaa atttaatttt gggcccttgg
     5821 gaacaattcc cgtaggatta atgcttaaat gacttcgata ataatgttgt ttgatatgat
     5881 taaaatttac ggtttctttg ataccaggaa attgataaag ctctcgtaaa taattggata
     5941 aatgcggata gtcggcaatc cgctgcaagt tgcatttgaa gtgaccatga tacactgcat
     6001 cgaaccgaat taatgttgta aataaacgcc agtcggcttc ggtgagttgc tctcctatca
     6061 aatatcgctg tgttgccaat aggttttcta tttgatttaa ggtattaaat aacgcatcaa
     6121 atgcttcttc ataagctttt tgggatttag cgaagccgca tttgtaaaca ccgttgttaa
     6181 tattttcata gacaagctga ttaatctcat caattttttg gcgccattgt tttggataat
     6241 agtcatgatc tgtagaacta aaggcattaa attcgctatt caacatgcgc atgatttctg
     6301 atgattcatt attgactatc gttttctttt ttttgtccca taaaacagga actgttactc
     6361 taccggtata atcttttctg gcaaccaaat acaactcttt gagataatgg atatgattga
     6421 tttcatcggc tgtttcatta cctgattcgc cgtaactcca gccattttta cccatgactg
     6481 gatctgaaaa agaaacagaa ataatttttt ccaatccttt cagttttctc atgattaacg
     6541 ttcggcatgc ccagggacaa gcataggaca ggtataaatg atatcgatca ggctctgctt
     6601 taaacccact ctctccactc gcagtaatgt gctgacggaa acctgattca gcacgaacaa
     6661 aacgtccttc tttgtccgtg ttgatattgt tttcatcgtg ccaaatacca tcaattagta
     6721 tgcccattat gaattcctta tatatcaata acttattaat attttttgaa tactggcgca
     6781 gcatgaatac gtcttgttta ataactgctt tttaatgcat aatcattgat aactcgatta
     6841 attcattttg tatcaataaa tttaaggaaa aatttatgtt caacgtagct gttattgtcg
     6901 gaagccttcg taaagagtct tttaatcgta aattagcatt tgctctggac aagcttcagc
     6961 accctacatt tcagctatct catattaaaa tagatgatat tcctctttat aaccaggatt
     7021 ttgataataa tttacctgac tcagtcattc ggttaaaaaa agaaattact gaggcagatg
     7081 ccgttttatt cgtgacacct gaatataacc gttcgatacc cggtgtattg aagaatatta
     7141 tagattgggg gaccaggcca tacggaaaaa atgtatgggc aggcaaacta atggcagcaa
     7201 ttggaacgag tccgggagct attggaaccg ctgttgctca atcgcatttg cgaagcataa
     7261 tggttgcaat tgatggcgtt tattttgggc agcctgaaat ttatcttgtt tataaagatg
     7321 gactgattga taacaatttt tctattacca tggagtctac ccgaaatttt ctgcagggat
     7381 ttctggatcg ttttgccatg gaaattgaaa aacataaatc ggtgtaataa tgccaggata
     7441 ttgtcttaaa acaatgttat tggcaggcaa ctacttttgc attgacatgc catgttccct
     7501 gttatctatt ttagctagtg ctccatcttt acctacgtca tacttttctt tatttttaag
     7561 ataatccatg acttcagcat ttgtaccgtg aacaaatacc cccttaccat ctgagtaaac
     7621 acgatgatcg atcctggcat tatacatgtc aaatgcacga cctttttcag attgagattt
     7681 aaaaaagtca acagcttctt ccttgctatt aaaactgagt gcaaaccctc cttgatgggg
     7741 cataggctct ttaccaaatt catttttgta gtcttttaaa agttcaataa gctcgggttt
     7801 cgctccttcg gaaaaaatat aattgagttc aacctcttct ttcaaagatt ttggatgagc
     7861 aattatctca tctcttaatt gattgaaaag tcgattatat tcttcttctt cctgtagttg
     7921 ccgttctttt tgatgatgta attctttaaa tcgtttgata ttcgtgtgct ttactctgtc
     7981 ttcttcagtt tccatcgctt ttctaataca tttcaattca aacctgttta aattatatat
     8041 tatttggtat tggtgtggtt ttgatagtat atattttaca caaatttatc atttgatctc
     8101 ttgttcaagg gaagtagggt tattatattg atttataagg ttactttgta atcgtccgtt
     8161 taaatctgat acaattaagc gctatttgta tttttcataa gtaatatttt aacaggaaca
     8221 ccattaattg aattttattg gctggacaga aagacaaagt ctttttcgat tatttccgct
     8281 cattatcagc atttcttttg ctatggatgt ttttgtgccc gcaattccgg aaatgagtcg
     8341 ttttttcaag actgacagta caacgatgca agctactctt tatttgttta tgctcactgt
     8401 agctttgggg cagttattgg ttggaccttt ggccgatcac tttggacgaa ggcagatggc
     8461 cttgagtatg gcacttctct tttttgcagg atcgttgatc tctgcgttgg ctccgtcagt
     8521 accggcattg atttttgcac gtatcattca ggcagctggg gcctgtggaa cctatttact
     8581 gtgttttatt attgttcgag ataatttttc aacgggcacc tgtgcccgct tgtttagtat
     8641 cttgtctggg acgaattcaa tggttgccag ttctgctcca gtcattgggg gattattgtt
     8701 ggatgtgacg caagattggc atagtggatt ctacttttta accttgcttg ggttattaat
     8761 gagtggcata gcctttcgtt acatccccga ttatcactat cccaaacctg agctgcccca
     8821 gatgagggtg taccaagtga tgaggcagat aataagtaac tctgactttc gccaatacac
     8881 attggttgca tcagccagtt tgctgggatt gtacttgttt tgtgctcaat caccaggtat
     8941 tttgatcaca caactgcacc tgagtgcgac tgaatatggt ttctggtttg gtttgaatgc
     9001 aatggctgtg tttgttgcca atatgatggc cgcacgactt acttattctc actcattgga
     9061 aagcatagtg cgttggggct tggttttaat gacactgtca tgctttttta tgctggctct
     9121 taatcttcgt caatgcagca ttttaagttt tatgcttccc atgctttgct tgactgtggg
     9181 tattggtatt agcatgggca cagcaacagc gcttgcttta aaggattttg aacagcaagc
     9241 tggtgtcgct accgctctgt tgggtgcttg ccagtttggt ctcgcgggtc tgattggtgt
     9301 tttgacagct caatggatgc ccgggccatt aagtgtagct atccccgtac tttgtttcac
     9361 tgcagtggga ttggctagaa tcaccaaatt cagttttaga atattgagcg gctcttaatc
     9421 ttgtccgcag gcaattggcc aaaaaagacc gattaccggg tatgcggtag gtaacactgt
     9481 aaccttcagt gttgtttatc ctgcatgtgg ttctgtatta aattttagtg gcaatttcac
     9541 taaaaaagga aacatcattg ataccatttc tatcttgact catcaagcgc aggatatcac
     9601 tcatgaaggt cctggcgctc gtttcattgg acatgatcgc tttaaaaaag tagctgcatc
     9661 caatacatag aatgattttt ccaagcaagc ttacaataag cctcatgtca agaaatgagg
     9721 ctttaatgat tctgtagatg ttctatagac aaggaatgaa tttggtaatc ttaaatctat
     9781 tcggtagtca tttcatgcaa attattccca tacagtagga cagcaaaact gacaagccac
     9841 acgtaaaacc cgattaataa tttttctaca agtataaaag gtgaaaccaa tgccagaaga
     9901 aaagcagcgg taccaaaacg ataactgctt ttagagccat aggtaagtaa tgaaaataga
     9961 tacaaaaggt ttgccagcca agcaggttgc catgctagaa aacccatcca ccctgttatt
    10021 aagatttcaa atccataatg tgtttgatgt atccctcgtt caaaatcagg tattattgcc
    10081 gcaggcaagc atagggcagt gacataaagg attattgata aaaagataca tactgattta
    10141 actgaatttc gagtcattga ttactcgctt tttttgatag aaagtaacat tacttattca
    10201 gcgagctcaa gaatttcatt gagcaattta atgcggtctt gaatcaaaag cacttgccag
    10261 ctttccgcgc taatcttggg gcatttcaca cgagccagtt gaatccaggc atccctgtat
    10321 ttaatccagg ctatctcggt ttttttaatg ccttctttgg taaccgttgt gtactgaaag
    10381 gatgaagtat ctgccatcaa ttttttatag atactattca attgcttatc catttcctca
    10441 tattgtttta ttgtcataag aggagtttcg catcgattta ctttttgtaa cagttcaaac
    10501 aagcgttgat tcagattcaa ctgtgcattg atggcaaatg cgcttctggc agttcctgat
    10561 aaatcaattt cattcatcga atgatcacgg atatacactt cggcagtttt tctaacctgc
    10621 tgatataata attgttcttg agcagtccac tgactgacta aggccttaat tttttgattg
    10681 cgttttacat cttccaggcg ctgatcaata gaagagcacc aacccatcat atacccactg
    10741 gtaatgtcat cacaaatatc aaaagtagtt ttcttattac cctcttttat ctgtgcgaga
    10801 tgggcaatac ggccatccat ttccgctgga gcggcatcct ccatcatgca ggcaaaatgc
    10861 atggcaagat caggattaaa tgttactcct ctaccattgg cgtaagccat aaccaggaca
    10921 ttataatctt tattcattaa tgcgcattga aaggctttgt cataatcggg ggcggcatta
    10981 aaaccataat agagatcaag agcatcacag ttttttagtg aggctttact ggcctcatca
    11041 agctggtttt ggcttggtac tgatatatga gctactgttt ggcataattt ctttgtttgg
    11101 ggagaataac tctctaaatt aaattgattt tgctctccag tttcatctgc ataaagttca
    11161 ttaatattgg ctaaatttaa taaaccaatt aatagaataa agataatcca attaaatctt
    11221 atgttgttag ttatcataat tttttctttc taattatttg ttattaatac ggcattccat
    11281 ttttatgcat aaaatgccat tgcccatcat taccaaaggc tgctgccaga tcatcggcag
    11341 gataatttac ttcatcgccc tgggttcgat ctcctatttc aagataaacc acttcatcag
    11401 ctgtccgatt gaccaattga tgtgctgtgc catctggagt aaatccagca cacattccgg
    11461 gatgtaattg aatgtcagca gtttcagtga ctaatgttgg ctgccctttg aggataaaaa
    11521 caaactcttc ctgtaattta tgtttatgta gcaacgcaga ttgtgctcca ggagctaatc
    11581 ttgttaagtt gacgccaaaa ttctttattc cgaaaaaatc ccctaatgca tgtttttgac
    11641 gaccagtcat catggatgca aacggttccg gataaatgga aggtttggtg cgagggggaa
    11701 cctcagctgc tgctatagca agcaatgaat gtttgtcttt gttcatcatg atcgtcaatt
    11761 tgagttctat ccttgcaata tttaatcaat attagcataa agtaaattat cttgaatccc
    11821 tactttcagg aattctagat tatggatttg ttactgaatt tagcgaaacc aaagcaagat
    11881 taatcttagt taacagaaag taaactatag agaaaggttt tgagtgagtt agatttttct
    11941 atgaatctgt ttccggatct ttgctttagg agtttcaggc taaaaaatta attatcagcc
    12001 agcacaatag atatatcatg aactctaggg gagtgatgta aggcgcactc cttttagagg
    12061 agggagcgtt tttacagagg gtttcattgg ggtataaatg gataatccat tcacgagaag
    12121 tgctggaatt ggtgtagata atttttttcc gataggttct gaaagcggag tagatggttt
    12181 ttttactata gatggaagag taggaacaga tgattttttt gcagtcagtt cctgggttgg
    12241 agcaggagat ttatttgcca ctggtgctgg tgaggattta tggattggga aaaatccaac
    12301 ctggcgagcc accaaagcgg tttgattttt tagaaccggc gcgctcatta ctccagaacg
    12361 ttgatcagaa aatcccagag ttcttaatgc ttgagacaaa tcaacattag tcaaagaaag
    12421 tctggcagca aaccaggttt caatggatgt ttttgtgaaa atctttgcgg caaataattc
    12481 acatagcctt gcatttcgag tcagactatc tgttcccagg ttatcaaaat aaaaattaat
    12541 cattttggat tcaatcaatt tttttgcttg ttcctgactg catccccgaa tggttgcttt
    12601 atgatgaatc gctcgattca tgattaaggt aaaaaactcc ggatcttcga aaacaatgcg
    12661 tgctggtgaa tttttctttt ttgcttcaag tgcaaggtca acaatcatgt tctcagtttt
    12721 attttcagga ggctcgtttt gaaaaattgt tttaatcaaa tccagtcgag gagcgttaat
    12781 tatcaggtgt tttataaatt tttctttatc accctttgta cctaccattc cttgaatcag
    12841 ttttttttgt tcgtcagatg ttaatgaatt gtatagggag ggcaagtaag gcatttgttt
    12901 gtgaagatta atacacgctt gaatattttt agcatcggat aacaggctta gcttttgcat
    12961 ttgcaagagc agggtaaaca attccgggtt tgcaaaaata tattgacctt gccctatcgg
    13021 attttttaat aaaatacgtg tgatttcata tctctgttga ttgagactga aggtacaaaa
    13081 acggcaatct cgtttttcaa gttcttctag actgaatgtt tcttgctttt gattatccca
    13141 tggattgact aaaataaaat catagccacc atttatttta gggaccactt tatctattct
    13201 taacgcatga cggtcatgaa tttttccatt ggcatctggc tcaccatagg cgaagctgat
    13261 ataaacggct tctttggggt tcattaattt gagtttgatg attgcatcca tttcttcagt
    13321 atcttcagca tgaataccta ataatttgcc gacaaaacgg gtggaggttt ccatgtgtct
    13381 ttgtcttatg ctatgagctg tcagacttcc agattcatta gtatgatccc aatctccggt
    13441 gtaataatag gagctgattc gttctaaaac tttaatagcg agtgaattgg tttctacccc
    13501 tttttttgaa ttatcaattt ctttgagttt ttgttggcct ataaaaaata catcttcatc
    13561 tgtatttcga tcataaacgt ggatatattt accgcgcatt ttctgagaat caagtcgggt
    13621 actctgatta tttcgtttaa tgcggagagt tagtccaagt ggagtttgtg taaattttga
    13681 tgctaacaat tgacgcgctt cgtctccaga attaaaaata caatctaaag caactaacag
    13741 gtaacaatca tcgactgggc cttgttttat agtaagggga gcgttttttt ctggaaacaa
    13801 aggcgccata tagatctcca attatacaaa aagcttttaa ctttgccatt ttatcaaaga
    13861 aatattatgt taatattaag aaaagtcgag gcaagaatgg tttaaaccca tcattttctt
    13921 atgttcagga cttacgaaaa cccccaaggc cgaggtaaag acatttttta acaaagagat
    13981 tgggggttat cgtaagttct gatgttctcc agcacccggt tttttttctt gaatgactat
    14041 ctcatcctta atttcatcat tttcattgat gaattggatt ttcatggcat gttccaaagc
    14101 tgttatcagc ataaatccgt ggtgtttaga gtaaaaaaat ttgcttcctt ctacccattt
    14161 tttataggaa taaagctcag ctcctccgac tccattgaca aaataaacaa tcccgttgcg
    14221 ttcgatacgc tcgtaatgat gatcatgacc actcataacc acatcaatac ccatagaggc
    14281 aaaattccat tgcatacggg tattggaacc atgtttacca gaactcaacg gagcatgatg
    14341 aaaatagact attttaaaag gagcttttga ttgttgtact tgttcagtta accattgata
    14401 ttgttttgaa ccttccttgc ttccatccgg ttcgtgagag tcactgtcca aggcaaaaaa
    14461 atgaattggg ccgcgaacaa aatcataata actttgattt cctggaaggg taaaataact
    14521 gaaataaggt aatgttccct gatataaaca ggcttttcgg gcaagccagt catgattacc
    14581 cagagtaggg aaaaagcgat tgacatctga acctttcccg tattttccct gataattgcc
    14641 tatgtattcg tgatagtatt tgcccacatt cttatcaatg gttttccagc aaccgtgagt
    14701 gtaattatta tctccaagag taagaataaa ctggggattt tttgacttga gaacgctggc
    14761 tactttggct tccgctgcag aattggcacc atagtctcca atgacagcaa aaaccaggtt
    14821 ttccttggcc atcagcgaat tagtcaggat aactaaaaca agaaacaaca aatttttcaa
    14881 agaagatccc tgtgcttgat gcagcgaagc cgctatggta agagatctct ttttaataaa
    14941 tcaatagaat tgaaagtaca aaatgattaa gtcaatttat ttttaaactg atcgaaattt
    15001 agtgaatatt atttcattga taagatgagc taagcgatgt tatggcgtta caaagtcctt
    15061 ttaagctgtt gtaatgtgat gcagtaatta ggggtgtatt gaacaattct gtgtttgctt
    15121 tgttcaagat agtagtcatg gaatcgccag ggccgagatc tacaatcaaa ttatactgat
    15181 attcgccgat taaagcacaa actttatgcc attgaagcgt tgtatatagt tcttgggcaa
    15241 gcaaagccct ttcttgcttg gcattatata ttttatttag ttctagcggg cttatgacag
    15301 ggtattttag agaataaaaa ccataatttt catccagaaa tcgctgaaat tgtttgcttt
    15361 tatcagagta aaaatccgta tgagaaggca gatacactcc taaaaatttc gcgtgactta
    15421 tagggtattg ggaaagctct gtgatcaagt gccggagatc tgaaattttt ccgccgatca
    15481 ccacatgttg tattgaattg ataatagcaa tatagcagtt atgttttgca ataacaggtt
    15541 gaatttcttc caatataaat tttccaacga cagacaacaa atcgtattct gcaaagatat
    15601 tgttatcaac tatcgatgtc atgagttggg tacgatacct gaataattta aaggaatctc
    15661 gaggatttgc attaatgctc cctaaaaaag cgctgatttc gcctaaactg taaccagcca
    15721 gatcaacttg atggtttgtg aagaggggca gcagtcttga aaaaagggtt aactgatagg
    15781 cgccaataat agattgaata gaatatggat tggtcatttc caggttattt tgtaaaaaat
    15841 caatttcagc tgcagaagac attgtcttta gatagttgat tgtcttttta tcagttcgaa
    15901 aaatatcaaa caaactgtca gcatgataac cttgcccggc aaaaagaaat aatattttca
    15961 tttggcttct cttgagaaaa catgccgtaa aaaataaatt aagcctaaca tatctgctac
    16021 gcccccggga cttatgtttt ttttggaaaa taattgatgc agttcaatgg ctttttgtat
    16081 ggatttttcc cgattgtttt gacatatgtc ttgttgaaca tgataacggg caaattctaa
    16141 tccctcggga cctgtgcgat aaaggatatt gatgtcgtca atagccaata ataaacgttg
    16201 gtatgctaat aatccaaaga atgtttgatc gtgaataatg ttcgacaact cattataagt
    16261 atggaataca gccttatacc cttgaatggc agtttgcttg gcatcaggta cagcatatct
    16321 tttcgttact aatgtaccat gggtatctct gttttgatgt tctttttcca ggtattgagc
    16381 ccattgctca ataaccgcat aatgcagatc ttcagtagaa aaggcatgat attttgcact
    16441 taatttgcaa atcgaagtac ataatatacc aagggcaaat atagctcctc gatgagtatt
    16501 gacccctcca gtaatcttta gcatggtttt ttcagcgttc aaaccaaatg ttaccagttt
    16561 ttttggtgaa gatcctaaag cagtatgtag gccaatctgg aaaaaataat gacgcaagcc
    16621 aaatagactg cgaaaaaata aagccccatt catatcctgg tgagccccac tatcaatgaa
    16681 actcactaat ccaggttttg ggtataaggc tacttcatca tacagggctt gaactgcctt
    16741 ttttgcaaaa tatctggcaa ttttgtatgg attaggcaag gagtgttgga aagtttgcat
    16801 ataggctctc tcttttcact aaaaatattt tttttgctgc tttaaataaa atggaatcac
    16861 tggagtgggt ttgtaacaat tctgcccaag aacagtctcc taaggccgga aagcgtattt
    16921 caccatcgat acaatcaatt ttaagcattt tttgtaaatg tttatgcaat tctgacaagg
    16981 tgaggagtga ttgattttca taagatatca gcacatccag gtctgaagaa gattgaatat
    17041 attgctcctt ggttaggtgt tgccagcagt aggacccata aacctggatt ttggcactag
    17101 tatcaagctc taaataaggg aatatctctt gcagagaagg gagtgctttt gtgtcaatta
    17161 tccaagagtc atccactaca taactggcgc gtaatttttg gctttgcgtt gggataaagt
    17221 agggaatggc aagttggatt tgtccacagg ggacatcttc aggctgcctg gttactataa
    17281 gggggtattc acattttaac caatattgga gtatatcaag ttgttcagga gcgggtaaga
    17341 ttgcggttgt cgtgttttta tcaagatagc atagagtatg tctgggatag ttcatagatt
    17401 ttctatggct tttataatgt cataggcttc tttgcgaccg ccatattgtt tgcctaattc
    17461 ggcacgatta tcatggctaa tattgggttc ctggagtgtc aatagcaatt gctgagagac
    17521 ttcatccaaa tcaagaacct ggtgtaaccc tcctaatttt ttgaagtttt ctacgcctgg
    17581 agcaaatacc ggggatgtct cactgatttt tcgtaacaca tcgatatcta tcttggtcac
    17641 tttagacata ccctctagcc acatgactgc caatttagca tttttcaaag catatattct
    17701 gtcagccatc atgccgaatg cgataaaact gccaccaatg gcttgattgt atatgagggt
    17761 aatgatttta ttgccatttt ggcgtgccaa atgcaaacac tttaataagt gagcaaaata
    17821 tgcatacatt cctagccatt catcacgaac tgataatttt tgtccgacta catcagtcaa
    17881 caggacaata ggatctgtat tgttattttt tattatatcc aaaatacatc tggccatttc
    17941 tatcgactcg tcaactccga aactggtgtc ttcaacggtg ccaacaatgt tgaagttttt
    18001 gttttttatt ttcccggatc caaaaacaat ttgctgttta attgttgttt ttgttgtgtc
    18061 gaagcaatga tcaattaatt gttttaaagt aattttttca ttgggcatta tttatttccc
    18121 tttattagtc aatgctagga attgctggtc agaaacattg aatatgtctt gctgatataa
    18181 ttcagttcgg ttgtttttta accactcacc ttcctcacgg caatttttgg ctgaggcaat
    18241 gcgtttttgc agttgttcat gctctgccag aatagaattc agattaaggg atggggcagt
    18301 ggggagcgtt ttaagggcgg tttttatcgc ctgacgaata tccatggttt tattggtggt
    18361 gtatccttga acatcggctt taagaaagcg ggttctacca ccatagactc gccagactaa
    18421 tggacgatta ctgctatcaa atacttcaac tccttttact gcctgaatta cttccgctcc
    18481 tgatacccca atacgtgctc cttgattaac gataagataa tcaagggtgc cacttatgat
    18541 ccccatgcca ccaaaagccc cgttattgcc acaaataacg cctatggttt taatgccggc
    18601 tgagcgagca tccaggattg agcgaatgat ttcagaaatt tcaatctcac ctacattcgc
    18661 ctcttgcaaa cggactccac cactgtcaat caaaaaaatg attgcaggaa gttgattttt
    18721 aattgcatac ttgattagac cattgatttt ggcaccatgt atctctccta ctgacccgcc
    18781 aatgaaatct ttttgttgcg atgcgatagc aatatcaatt ccatccattt tggcggtgcc
    18841 aatgatgacc ccatcatcag tttccacttg catgtttaac tgaggcaagg ttggacttgg
    18901 tgtctcttta tcaagtaaaa actctgtaaa acttgcttca tcaactaatg aataaattct
    18961 attgcgagcg ttcgcttccg tatagctatc ctttttagga tatcctgttt ggtaggtttc
    19021 cattgcttga cgcaggcgta aggaaacgac gggaggggta gcgcccgcat cgttaagagt
    19081 aatggatagc ccgctatagg gatagtcttc aacaaagcgt tcgattacag catcccaggt
    19141 atttcggaaa ccagagatta cagtatttac aataatctcg gttttatcaa agtcagagtt
    19201 tggtttaata atcacttcca aattacctga accggccact ccaactttaa tcattggaga
    19261 tatctggatt tttttgggca aactgaatgt gtaatgatat ttatccataa ataggtctac
    19321 caattcctga atttgttagg aggacaataa agtccatgcg aaatttccac taaatcatgg
    19381 atgtttttgg cagcgagtag atctcgatca gcttctttta atgaaatgtt gagatcttct
    19441 ggtcgttgaa tgatgccacg atggcgcagt tcatcgacta ttgctttatc acgtttcaaa
    19501 cctaccggag tataaccggc gattccgcgg atagcttgtt cacgctcttc tgaattttta
    19561 cataatagca gattggctat cccttcttct gtgacgacat gagaaatatc atcaccataa
    19621 atcataatgg gcggcaggtc cgcctgcatt tctttttgta gttcccatgc atccagtttt
    19681 tctacaaagg ttggtttgcc tgccccctga aatgtttcaa ccatttgtat tacgagttta
    19741 cggcctcgag gcattgctac aggattatga gagttttttt cttgtccggc tttaagccat
    19801 gcgtaagaag aatgacgtct tcctaaagta tcacagccca tattgggtgc accaccaaat
    19861 ccggcgattc ttcctttagt tgctgttgaa ctgttggctt gcggatccat ttgaagggtc
    19921 gcgccaataa atacatcaca tccataatgc ccggcaagtt gcccatacaa gcgatttgaa
    19981 cgtaatgaac catctcgacc agtaaaaaag atatctggtt tggagcgaat aaattcattc
    20041 atccctacct cacctcctac acaaaagata gattcgatga agcccgcttc aattgcaggg
    20101 attagggttg gtagaggatt gaccatccaa tgtttgcaaa ttttaccttt taaacctaga
    20161 gaatgggcat aagttggcag aataagttca attgcacaag tgttaaaacc aactccatga
    20221 tttaatcgat tgacttgata aggagcataa atgcctttta tcaccatcat ggccatgaga
    20281 atttttactt cgtcaatgag agctggatct cgtgtaaaca aaggctcaat gtaggaaggg
    20341 gatggggctt gaacgatggc gtcgacccac tctcctggaa tgtcaattct tgggagtttc
    20401 ttgactattt cattagcttg tacaatgacc aggccatttt taaaagcagt tgcttcaacg
    20461 atactcggtg tttcttccgt attggcaccg gtgtacagat taccgtaata atctgctttt
    20521 tctgcagtga ttaaggcgac attgggagta agatccatca cataacgtgc ataaagctca
    20581 ttgtaagtat gtatattgcc tatcttaacc tgattacgct ggatcataat tgccaaacga
    20641 gttgattgtg ggccagcaaa cgcaaaatca attttttctg caattcctcg ttcgaacaag
    20701 tcgagatgtt ccgataaggt aacagccgat tgcaacatat gtaacccgta tattttttgg
    20761 ggatttacag aacataggct gcgtgccaga aaatcggcct gtttctgatt gtctccttcc
    20821 aggcagactt tgtcatgagg ttttattgct gtttcaagaa gggcgactat atttttctca
    20881 gaaacgattt tacctttttt aaaaaaaggt ttgatgttaa tgagtctgct ctcataatcc
    20941 ttgaggtttt tattccagtc cataattttt tagtaatcgg atgagaacta tagtgatatg
    21001 aaatgttaat agtatagtag atattgacgc aagtatttga ttttatcctc tgcttttttc
    21061 ttgtggcttg tgcaaggtaa gttggcctat gatatttctc tgtaactgaa aaatactaaa
    21121 tattttgttt cagtgtattt ctgttttctg gttgaacaga agagattaat tccttgaaag
    21181 aaaacaatat attgcattaa cattagtgaa ctgtttaaat tcataaatca tgacataggg
    21241 aagtggatat gagagaaaaa tataccggtc gatcagagcc tattatcaat aaaccttctg
    21301 atattcccaa gggtttggtt gctaaaagaa aagcagagtt gctcttggca aaattagaag
    21361 aagctataaa agctcaagaa tctttaaata gagaatcaga gagtcctgcg aaggagggca
    21421 attctgagtc actcaaaccc cgattgaccc atccaacgag cgaacgtcca aaaatcaacc
    21481 atagacctcc ttcttccttt agctatacca attcagacag tactttgttt acttctgtta
    21541 aagaagaaaa aaccgagggg gaggtgtcgt ctgtatccct ttctaatgat aaaaaaaaag
    21601 ttgaagataa ctcttctgca tcaatttgga cggaactgat tgctgcctta tcaagagcgg
    21661 ttgacaacta tacgaagaaa aaaatggaag gaactactcc cggtgaagct tatggttggt
    21721 tcagctggtt gcgtcatggc aaggcagggg aagataaagc aggagggctc gcaagtgaag
    21781 cacggacaac cagtacggtt gaatctttgt tgaccttact tcatcatttc tttgcggatt
    21841 ctaccactag atacaataat cattcattgg ctccttatgt gatggacgag ctcaataaat
    21901 tattgattca gtatgctctt aaaccgttca tactcagttt aggctcccaa tatgatctta
    21961 atagctggct gcatatcaaa aatcaattaa ctcaactggc aggtaaggaa gcgtgtcaag
    22021 aaaagcaatc tcttgttgca cctgtgtgat tatttaggag tcctgcatag ggctccatat
    22081 ttttaatcgc tattaaagtg ctatattttt tgcaatatat gcaattttgg cactttcatt
    22141 tattaatggc catatctcat cgccttacct tgcaaacttg aggggatgaa aaaaatatcc
    22201 tagactttga atagctgtcc aaacgtttaa gagaataaaa tgaatacgaa agggatttta
    22261 tgtgctgttt tattgcttgt tggaacagtg cttgcagaga ttggctatgc tgagttcaat
    22321 gaaaatggca acagcattgt ggcgaccaat aactcgcaaa atacctcaga acaatcgtct
    22381 caacaaaatc ctaaacgtag tccttcggga caaaactgta ccggaacaag ccctaaccca
    22441 ttcccacaaa atcctgcatc gtctcctttt tcgatagggc aattgggcgc ttgtgttaat
    22501 tcaactgcat gcaatgccac caatgcgaca ttgaaaagta aaattgcaga caggtattgt
    22561 caagacgcta acaaggcgac ttgtgatgcg aatgatgact gtctgccgcc tcccgattgt
    22621 aaaggtcgtg tgaatggaac agcttccagt attgtgctcg ctaactgtca aaatgctggt
    22681 cccacccttt gcaccagtac tggaggaggg atttattgcc tttgcgatgc ggaaatcgca
    22741 gcgaatgccc aaattaaatg tggttgcgaa tgtactacta aaacactgcc ttaatagcct
    22801 taataaataa attgtaaagg cctccctgtc ggcggatata ggtataccca aaaaagtctc
    22861 tttgagcctg tctctttcat tttctgtaca taatttgact catttaaaac atcaccagtt
    22921 ttttaacaat ttttgtgctt cattatgggg gcatggagtt tttattaagt agagtacatg
    22981 ttgtatttaa attataaata ttgcgctttt tatacattgt attttatgtt attgttcaat
    23041 ttcttgatca ataaaatcag gaagttagat acaaaaacta ttttggaata gtccttttac
    23101 tttacttgga atttaaaaga tgaaagaaat tatcgagtta ttagctacct tggatgaatt
    23161 acaaaaacct tatattcatc gtgtcaaaag caggttaagc gagcttttaa atctggatga
    23221 aaaagaaaac gagctaatgc gaattgagca atcgacaagc cggtcctggg aagaaggtgc
    23281 tacaaccgat ttaaggatag gtcctataaa atcaaaaaaa agaatagagg aaataagagc
    23341 tgtactcatc acattgtctg gcgccagcgc agtcaaatat gaggaggggc acaacttaga
    23401 gaataatact gtggagttta ttattacggc atgtgatgtt gagaaactgg aagggtttat
    23461 ccctccgaat caggaagaaa tgcaaaatac tattcctttt acaacgttta ttcgcatgtc
    23521 aattgcaggg aatagggggt atgtgcgagg gatttcattc gattctgcaa aactcgatgg
    23581 agagagttac cttggttcgt ttaaagaagg cgaactaacg acgtcgaaac aggagtggtt
    23641 ttccgttcta gaattattgg atttattaaa tcgtgaatca gaacttaatc ttattcaaat
    23701 acagaacgat ttaaaatctc ttttaggtgt gaagtttgag gtttattttc attcatccag
    23761 tgaagaattc cctactgatt tgcgtacagg aagcttggat cttaaagaga caggagctca
    23821 acaactgctg agatcaatgg atagcctcgt tttgcttgat tgtttaaaaa cagaaaacaa
    23881 agaacttgag gatggccaga aagtatgcat tatcaagcag gtaaatcctc aggaacttca
    23941 caggttttca ctaaaaaaag gaaagaatac taatgcaaat gctgagatgc tctccaatat
    24001 tatcgaactt ttaaagcgat cttttccaaa taatcaggta atccaggatc tggtcgtctt
    24061 tgatggcaga gacgggagaa gtatgtcacg accctatttt catattagaa cccaaaacta
    24121 tcaccaggaa ggtaccagtg attataaaag cgcgatgagc attctcaaaa aacttcagga
    24181 taaaacatca aaaaaaatca tttttgatta cagaaatgag atggttgaga tgaaagatga
    24241 atcaagacct atatacatga gcagtattga gaatactatc aagtcatctc aatttattat
    24301 tgatgttgct gtcggcttaa caacagaaca gctacctgaa ttgcaaaata tatgtgctga
    24361 aataaatcaa tccaggttaa aacatcatcg tcataatctc ttcaagccgc aaattgagga
    24421 acaaaaaatc aatgagcaat gtcgagtttt attcataggt taaagtcaaa ttcagcataa
    24481 gaggcataaa atgcttctta tgtcgctact ttaacattat gccttgttat cttatcctgc
    24541 aatatttttg ttcaactggc attttggcat tttaacaaac cttcaagatc agctagagcc
    24601 ttttcgtcat ggatagttaa tagtgtgacc caagaggcga tgatctgatt gtaggtaaaa
    24661 atccgcttta accactgttt tttcttttct tcttcagcga tgtgctcggt taaggaaagc
    24721 tgctttttgt ttagtttttc ccaacttttt attgcgttcg ctagaggatc tttattatgg
    24781 gctcgtgccc attggtaaat aatcgcgttg cgattttttt gatagtccac taaatcagga
    24841 tcgttctgtt tttctgatag atattgcaaa taatacattt caaactgata agggtgttgg
    24901 tataaattaa gtttaacaat agtttggtaa aattcttttt caaatgtttg ttgcttctca
    24961 ttgagaggct ggctttttga ttgattcact gctttttccc aggatggcat gatgacattg
    25021 agtgtcagat tcatcattcc ctcccaaaca gcttgtaatt ctttgccttg ttgctctaat
    25081 tgcttttcca gcgaactgat tttttgtttt tgttttgtta aggataaaga agcaaattcg
    25141 cgggcttcgg gcattaactg gacgatttta tcttcagaag cacgggcaat atgcataaat
    25201 tggtaaaggg catcattatt gctggactga agtcttttct tataattcag gtaaatggat
    25261 tgaatcatat tgttgtcgta atgtttcgag cgggacttaa actgttttga tgcaaataat
    25321 ttggcccatt ggagtaaata atagtccgaa gtacttaaag gaatgtctac tgtcatgtct
    25381 tcaatgatcg cgttttgatg ggcaaccagc atacttttag ccggttccct gatttttttt
    25441 ccatcaatga cgccatgtcc tgtgtaccat tcggtaaaag aacgttcata tccttgataa
    25501 gtagcgccgt tggcatcagc agtgcagcca ctggcatagt gatcatctgt tgaggaagta
    25561 aaaccacaaa ttttagtatt agctacagga tagccatctt gtgttgtaac agataattga
    25621 tgaaacccac ctgagtaaca ctgagacatc gcaaaaataa cgtgttggct tggtattaag
    25681 gcgagcagcg acgataattc atttttggat aaacaggctt tgctaaagtt tttggcgtta
    25741 gtgaagttgt taataaatgg tttttgataa tgccagagat caatgcaatt gttactgtag
    25801 ggattggtat ttttatcctc taaaaaccca tgaggcatgc catgatcact gacaaagaga
    25861 agcaaattat caccagggtt gatttgttga attttggaag ataaaaaaaa ggcatccaca
    25921 ttgtatttat tggcttgttg attgtttgcg ataatacctg taaacatgac gtcttctttg
    25981 ttttgttgat tatcttttgt attaatgagt ttgtgcacat caaatggaga aggttgtgtg
    26041 gtcggtgtgt tacctcctcc aaaatagatg cttacggttt cgggattaaa tcgggtgatt
    26101 aaatgctcat aaagagtttt tgtttgcagg tattgggtgt agtggttaag gcctgggtta
    26161 tctcctcctg acagtaccaa aacttgctgg ccagcataag tagatgtggc gtgcagagca
    26221 attaaaatgc tgagaattat tttcatcttc attccatgaa tcgttgtttt aatgcttttc
    26281 attgttgtgc caaggatagc aattcttgta ttgaactgct aatgataatt gaagcgttac
    26341 ggtaaaaaat taagactaat tagacatagg taaaatatag tcagcactca gcactgttac
    26401 cgcttgtttt ttgattatat ttctctaact ttacccgccg tttgagctca atgcaggtgt
    26461 taaatgagtc tatttttaat aaatataatc attttgattg atattcgggc attttttgtg
    26521 cgtatggttt aaaattatat caaggggctt ctatttgttg atattcctgt tatgagcaca
    26581 tccgaaaaag acgttcgtga acaacaagta aaaacagtca cattaagttt tctgggtaca
    26641 gggcaacatc gtgaaaatgt tcatcacata ctcaccagtt tccacaacac aatctcggaa
    26701 gttcataaag acaaccctac agtggcaaca aggatgtttg atggccctgg ttcagaacca
    26761 aagtcaagcg gcagcaagga tccaattcca gggacttata tttataaccc aaaagataat
    26821 tccaagatat taattagtcc tgtaatttcc caaacgatta ccaatgccat acaaaaattg
    26881 acaggaaatt tggcaggaga aggtatagag catttattat ttgaagcagt actttacctt
    26941 aatgacatca ttgaaaaaaa tggaggcaaa ctgcctgaaa cagttaattt gcatgggttt
    27001 agccgagggg cggatacctg tatgcgcatg gcgaatcttc tttatcaatt atatcctgat
    27061 atcaaggtca atttatttct tatcgatcag gttcctgggc ctgggaaacg ggatgatcct
    27121 cattcttata ctgttcctcc taatgtagag cattttgagt cgacattgat gttacatgaa
    27181 tacaggcctg gatttgaccc tcaacattct ggaaggtatg tgattgcgga tcccgaaaaa
    27241 acaaaggtag ttgttaaacc gtattatgga gaacataata ccggtaatcg tgttaccgaa
    27301 gatcccaaca caaatcacac cgctatttta ttacatgatg atatgaatcg cttttgcagg
    27361 gaaaccggct cactcccttc tgttgggatt tctccaccca ttattgccag agtaggagat
    27421 aaaaaagagg aggttcgcac tcattcagaa ttatctccag aaaaacgctt tgagttgtta
    27481 tgtggtatga aagaaaatga gtcagaatat gccaaattga aaaaaaaaca ccatgaacgc
    27541 agtattcttt ccaagcagga agattacgta caagactcca gattgtttgt caaccaggag
    27601 caccgggaat tatttaaaca attgtatccc aaatcattta attggttttt tgaaaaaaat
    27661 catggtggtc aaacccagaa ggaagaagtc atcgccgaat taaaatcact atctgaagat
    27721 ccaaggtatg agcatttttt ttcatcatta gcgaaacact ttcagattaa tgagaataat
    27781 gttgcaggaa cattgccaga accctcgggt gttgatcggg atgaaaaaag tagctacaga
    27841 caacctgcag tcagggacag gctttcttat ttacaacatt cgctgacctc cattgcgaac
    27901 tattatcact atcattgtga tgagaagaac tcaactaatg agtcagttaa aaatttgctg
    27961 ttagaacgag taaaagagtc aaggacaaaa ccggatagcg aagcaatcaa acatttggaa
    28021 cagactatgg acgaggtaag acagacactc gaatccaaaa atgaaaaagg tttcctttgg
    28081 caacagatta atcatatttc cccgaatact caacaatatt gtgaacaagt gaaggcagta
    28141 ctccaggaac acctggagca taatcaggtg ctatccgata ctcaaaaaga ggaaattcgt
    28201 aaggctatgg atagaatgga taatatagtc aatgacggta gcaaagacag tcagcagaaa
    28261 tatagggaaa taagaagaga ggtgattgag ttaaatgcca aagccaccac accggaagat
    28321 gaaagccagt atttaagaaa tcaatttcac aaagcctatt ttaggctctc aggagatccg
    28381 cacaaaaacc tgagtttaaa ctctctatct aaaaccttga atcaactctc ggaagctcat
    28441 tatggtgaaa ccaatatgac ggataaaatt attcagcgat tggatagtta taaaaataga
    28501 aattcattct ggaattcagt caaggaagtt ttgaactttt tcaatattcc ttttccaaaa
    28561 ttgcattcag aagtcaaaga gcaaatcgct gatgcacttc aaaaaagact tgttgatctt
    28621 aatgaaaaag gcatgggtaa tgatgtgaat acaatcacca gagagcttgg taaagcacgc
    28681 gaagatttaa ttgaacatta taagaaaact tcaaaactgg aaatggggga actggataaa
    28741 attatcaata aatcaatgga agaactcctt gtggcacgca aaatcaccac caaagacttg
    28801 gtccacgagg aagttagtca ggttaaatta aattaagcac tgttctgttt tggcaaagtt
    28861 atcataagca aggttttggg tttaacaata atgacttctc tagctcaaat tgcaaataaa
    28921 actgatcttc ttgttaaaat tttagtctga tcacttcagt agggtaacta tcgttatatc
    28981 tgccttcaca aattactctt cccccttttc ttttgtagag tccattgagg gtggagttca
    29041 gtgtttcaag agatattact tcagcgttca tttctcttgc aaggcttttt gctttttcaa
    29101 taattattcc agcgagcccc atgctacggg cacgttcatc aatgtacaca tagtccaaat
    29161 aagcattttt catttcagtg gtttctggag tagagggttc ttccacaatt ttctcggatg
    29221 atttctctct gttttcttca aaagagagct ttttttcaat gacagcgagc attccaaccg
    29281 gtagtccgcc atacatcgcg atataaacat gttcacacaa ttcctgcagt tgtttgtaat
    29341 gaaattgttc gtcatccaga tgcatgtatc cccaggcatt ctttgcccac attgctgcag
    29401 tatccagatg ttccttgcac tcggataatc tgacaaattt tagttcaggt cttgctgatg
    29461 tgaataaagt gatgtctttt ttcaaacttg tacctcttgt gaatatgaat taatcaattg
    29521 aaaaagtgct atatgatact gtttatctgt gctttatcaa cttgaatttt ggatgttttt
    29581 agagaaaaga gataattttt tttactatat ggggattgga gaacaaaggg ggatatcatt
    29641 tcgatcaggg gtgtgacatc agatcaagtg aaatgacttt aaaggaatct catgccactg
    29701 ctcttaagtc gagccgccct cctgtctcat caatataata attgtttgcc ttagttaatg
    29761 ccagtacaga tggaatagat aatagtaatg cgcaagcgcc ttgattacct aatcgcagag
    29821 ccaggcgcag taactcattc tcatttcctg gaatggttcc agcaggagcc aaagctttta
    29881 taccggggat gctcagcaaa aaacgaatat catccagtaa agcctcatca ttacgacgga
    29941 ttaaattacg taacatataa aagccttgta ggcactccga ttttgtgact aaatcaaaga
    30001 gatcctccgg gttactttgt ttaaaggcat catgcatttc ttttaacctg ttcacatgcc
    30061 tcgcaataaa cggattgacg tacttctctc catattcaaa ttcatgcatt tctgcatacc
    30121 cgagcatagc tgggcaatcg agcaaaaaat taatgacatt atgatggcct cttccaactg
    30181 ctgcccagcg aaaagcataa taattttctg actgaatcat tgccgtggct tcagttggcg
    30241 ccaattcaca taaacgattc agcacatgca ggtgtccatt ttctgcagcc agacgaaagg
    30301 catggtaatt ttctgcctga atcatcgcca tgacttcagt tggcgccaac tcacataaac
    30361 gatttagcac atgcagatgg ccgttttctg cggccagacg aaaggcatgg taattttctg
    30421 cctgaatcat ttttacaatc tcatcaggca ttaaagaaag agatattgta tctaaggcac
    30481 ttgagcaacc ggttattgca gcagcacaac aaataacctc gctttttatt cctttcttgt
    30541 gaacatcaca ccagaattgt ttgtcattcc tgtcaaaatg ggcatagtat agacacaaca
    30601 attgcctcag ctgttcctta ttttttgtct ttggtaaaaa gatatggatt gcttctttat
    30661 aagataaagt cataagagat aattcatata cactgata
//
LOCUS       DATNSN010000023        29568 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.023, whole genome shotgun sequence.
ACCESSION   DATNSN010000023 DATNSN010000000
VERSION     DATNSN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29568
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.023"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..860
                     /locus_tag="VAH76_000831"
     CDS             51..860
                     /locus_tag="VAH76_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1385478.1"
                     /translation="MITMGRSEMKIASAKLRELMKAVSEGHYETVNTILDKDPELVNQ
                     YVPPTYDSPLARVLNKKYIDYKMLDILVKHHVDFDYPINHHKETPIELACKNQDLQLF
                     KYLVQHNAPISEHAPHFLLVNSTNIKYLTEDKIKNTCEIIKLMGGLEAVSSKCNAEGN
                     RFEELARKSQLINRFGGVVRYDYMQLLQSVYPIVDREVDTPTINASTEKLTNLLNKIR
                     DQHISKEGYDQQNLKDSISLFFMTGGEIPPSRKVPESRFEEAGIDTPKNTL"
     gene            complement(1015..1626)
                     /locus_tag="VAH76_000832"
     CDS             complement(1015..1626)
                     /locus_tag="VAH76_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1385479.1"
                     /translation="MNKRNWTAYYNSTKQNTLPRKSLLKAIANFEKEKINLSKSAIDL
                     GCGAGIDVMELLRCGWSVIAIDSQLSAIDSMLSSAQELTCANKLKTIVSSFEALDSLP
                     KVNLINASFSLPFLKPVYFYKFWNIILKGLKPDGRFSGSFFGFNDFWNTRTDMTFLNR
                     EMICHLFSQFKIEYFQEEEKDEMDALGYYKHWHKFFIVAKKIA"
     gene            1888..2319
                     /locus_tag="VAH76_000833"
     CDS             1888..2319
                     /locus_tag="VAH76_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385480.1"
                     /translation="MHPDLKDLLDYRNHKIITRYNMDYPTAAMQAEEALHELMKFIWL
                     CHKHKADKLLYPDDKNLDFSCVIHAEMADIDKMWHTFLLFTRDYHEFCDTCLNGIFFH
                     HDPLIGENIPVSEEDYALELTRYLTYIHDHLGSETLIKWFQ"
     gene            complement(2321..2701)
                     /locus_tag="VAH76_000834"
     CDS             complement(2321..2701)
                     /locus_tag="VAH76_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385481.1"
                     /translation="MPHPDYSVYNEKCPSHNVLEGISDKWSILVISLLSQKIYRFGEL
                     KREIGGISPKMLTQTLLKLERYGFVQRQSYPVLPMKVEYSLTRLGKELSAILVLLTRW
                     TETNMDKIIKAEKTFCEQKLGCQA"
     gene            complement(2723..3610)
                     /locus_tag="VAH76_000835"
     CDS             complement(2723..3610)
                     /locus_tag="VAH76_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO1385482.1"
                     /translation="MHDTSALKKKLDYARFIHLVIMFMVAMLIFLFSTFPEKWWVLIT
                     VLSVSAGIEPGLIIRRAKHRIGGTLLALIILIPLLYLLQLNYRLISILFVLAIVGLSV
                     ATLNTRRYDISVFFITLVVFFLMAQTEEATSPRGPFEMVLNRGICTLFGVFIVLAGDY
                     FLFQAYRYSHRLYFFHQVMVYDFLNDIVRKIDESNTEKINTLLFVEKLRGQFTEHYLP
                     ISISSENLKLDFKTSEHTKKRIDIFQETIWEIRRLVFALCISEFVLHSSTASEQHLQR
                     FKLLMNKARTHFIQFEGRY"
     gene            complement(3589..4521)
                     /locus_tag="VAH76_000836"
     CDS             complement(3589..4521)
                     /locus_tag="VAH76_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385483.1"
                     /translation="MSMSKFIEWIDKVDPYAVQRIALYKSLFIATVMAYVYWVFRPTN
                     FTAFFSPFLLVRFYESPSLTSFKEKEHFLIFIGVVVVLLSTSFYLVYPFRVVFFFFSI
                     IALASVYFYVLKNYLPLRNVTMLIIASGATILSTEPPANWQIVYDIVGSSALSMIAIF
                     ICLRFFPNQYLSVWKRALAKFIQSLEQDIEGSFSHRGPRFMQEEMTHLGMLRQYRRLI
                     PKKYMIYTQRISINIRNIQFSLDNLYYEAKNEAFWHGVKENLYTLRCAIKTNTSCDTP
                     KMSIVPESKLQQYVMRCLQQAFSHWNRLCMILQH"
     gene            complement(4499..5536)
                     /locus_tag="VAH76_000837"
     CDS             complement(4499..5536)
                     /locus_tag="VAH76_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEO1385484.1"
                     /translation="MKKLMEYGGKIKEYLLRLKPKRWLTLTNVIIFIGFLTAIIYVFS
                     YLIPFTDNAFVVNNVRPVAALTSGYITELYVKNGDAVRKGQKLFTVFKKPYEYTVEQL
                     SADLAGAQAKLAVLKATYERDLKLSENEQKIYKKLAQDDQKYLKGYAIKSVSLITLQN
                     SQQETKAAKDKWQASLKQLEIDQHQITVQENEIKSIQARLKNAKVNLDLTDVYAQGNG
                     VIQNLFFTIGTPVNINQPLFSLVDQDNIYIQANFNETDLRDVRKGSKVLIFPRMYLGR
                     KIFHGVIDSDYWSANRQLVDDRTQLQNVINENQWILLPQRLPVIIKVTDPDPKYPLRV
                     GASAYVYVEVY"
     gene            complement(5676..7028)
                     /locus_tag="VAH76_000838"
     CDS             complement(5676..7028)
                     /locus_tag="VAH76_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1385485.1"
                     /translation="MMCMLKKLMFLLLLGAGLLSCGQSTDQQISNIITPKQFPSSPRD
                     YRPVQELPYLAWWKQFQDPELDRLIDTGLKTNMDIHIAMANLQQAQGELQQVKLSWIP
                     NVQILGGYSTNPALGVPGGFYGIWPSYILNIMQLYTQQKRAQYQVQYHQAAIDGLRLT
                     LIGQVAAAYFTLIAQLEQLKLLYKLDRDLKSLIALSRKEINIGLKNDIDLAQLQSNER
                     LIAAQIKPIKHNIVVSQNALRYLINENPGLIKSKNNFAQLDFTRFKPGSLPASVLSNR
                     PDMKMAEYSLKAARAGIFVAYSDFFPVMQLDDFIGEAHLPDSTFEQATDAYVNWTINP
                     NTLGKISVSKGVYNAKLAEYVKTIRLILKEVDNAFSANKRMREQFLSYRHAQEDYQHK
                     YKLQNGLLKSGLMSYKELLESKVYLDTLALSTNQAKLELAMSLVMLYQDLAGGYACSD
                     "
     gene            7115..7474
                     /locus_tag="VAH76_000839"
     CDS             7115..7474
                     /locus_tag="VAH76_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1385486.1"
                     /translation="MIKKIAFTMYPVQNMDRARQFYEKTLGIKVGKISAEGAWVEYDL
                     PQGGCFAITTLAQGVIPSANSGGSVAFEVDNLDELVFQLKSKGVQFKIDLFASPVCRM
                     AIAVDSEGNAFTLHQLT"
     gene            complement(7688..8119)
                     /locus_tag="VAH76_000840"
     CDS             complement(7688..8119)
                     /locus_tag="VAH76_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEO1385487.1"
                     /translation="MFCIDDRIKSSSFILGEWPLSTVLLKNDASYPWFILVPRKMDIQ
                     EIYQLEKSERFLLIEEINQLSLLVRDYFKPDKLNIGALGNIVPQLHIHIVIRSKNDPL
                     WPQGIWQSSMPSIPYPESELKSLLPTLGDLVRAYDKPQSLC"
     gene            8356..8589
                     /locus_tag="VAH76_000841"
     CDS             8356..8589
                     /locus_tag="VAH76_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO1385488.1"
                     /translation="MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLVP
                     GQAVTYDVVNGERGLHASNVVALSENAEQEEIA"
     gene            8700..9695
                     /gene="hemH"
                     /locus_tag="VAH76_000842"
     CDS             8700..9695
                     /gene="hemH"
                     /locus_tag="VAH76_000842"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEO1385489.1"
                     /translation="MRRGLLLLNLGTPDNADIRAVKLYLREFLTDKRVIDLPTIPRYI
                     LVYCLILPFRSPKSAQAYQSIWTEKGSPLLYHSQNLVTKLQSALKDEYKIALGMRYGT
                     PSITTALAELKDCHSLTILPLFPQYSSAATGSAIEKTLSYLANQEIIPSIKIIRDFYQ
                     RPEYIQAQAKIMKPYIKDNFHVLFSYHGIPERHIHKSGCDTLCPQTCTPIYDKNQACY
                     RAQCYQTSLLLAKELQLGTHQYTTAFQSRLGKTPWIKPYTDEIFAELISKGIKNIVVS
                     CPSFVADCLETLEEIGIRAKEQWEKLGGEQFILTPCMNDHPEWIKAIHSIVNEQI"
     gene            9823..10362
                     /locus_tag="VAH76_000843"
     CDS             9823..10362
                     /locus_tag="VAH76_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEO1385490.1"
                     /translation="MKKIKSVSHLLFLLFKTLAYGLPLVTTYFILFHFESMLNWGGWH
                     SLLSTPVKDPSHFSLLHRFIILLIEALPLSINVLICHQLAKLFRLYEHGYLFEEENIR
                     LIKNISIYLISGELIQLLYQPLITAALTFTNPSGQRIASITLGTANLSTLITAFIILI
                     ASWIIKEAHKLKTDALLTI"
     gene            10449..10700
                     /locus_tag="VAH76_000844"
     CDS             10449..10700
                     /locus_tag="VAH76_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1385491.1"
                     /translation="MSIIVNLDVMMAKRKCRLRDLAEAIGITEANLSILKNGKAKAIR
                     FATLEAICAYLHCQPGDLLEYQSTEDITLSKTGLNLRKL"
     gene            10810..12159
                     /gene="legY"
                     /locus_tag="VAH76_000845"
     CDS             10810..12159
                     /gene="legY"
                     /locus_tag="VAH76_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegY"
                     /protein_id="HEO1385492.1"
                     /translation="MATFFFKTNYYQEIMLKRIFFLMIFFVSQTMASVFTHEEVQILK
                     KHFLNNFQTNGAIVASPSQYNPNYYYDWIRDSAIAMGLVETWYEASQSARYKKLLLEY
                     VSWTEKIQHQADPIAGQDILGEPKFYINGNPFDGEWGRPQNDGPALRASVLIRFAQQL
                     LDHNEIDYVKSHLYNNTMDPQSMGTIKMDLEYIAHHWQDANFDLWEEVYGHHFFTAMA
                     QQKALTDGAILARQLHDRQAAVFYEMQANLINSRLQQHLDHQNKIIQATLLPHPGPQK
                     TLELDSSVMLGILTNPQKEGVFAPHHTFVRNTAKALHEQFNLMFPINKNRSGAILFGR
                     YPGDTYDGYQTNSIGNPWFILTATMAEYYFTMAHNLSLNSSNKLHIQNYLKKGDNYLR
                     LIKQYGPDLNLSEQINLNTGVQQGATSLTWSYVSVLRAIHLREQLENRIKTMGWNTY"
     gene            complement(12249..13670)
                     /locus_tag="VAH76_000846"
     CDS             complement(12249..13670)
                     /locus_tag="VAH76_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEO1385493.1"
                     /translation="MAWIVAIIGSIAGFLFGYDEGIIAGSLGLVKNHFNLNATHIGVM
                     ASALPFGALFGSLLIGAFMASKCVKRFGRRSLLSFAGFLFFVGALGAGFAETISVLIL
                     SRLILGLAIGMASVLTPLYLAETAAMQSRGAVVAIYQLALTVGIVCSYSVNYLLIEQQ
                     AWRAMFASSAIPALLLTLGILFMPESPRWLCSVGRHDAAANSLRKLRGKQSVEQELKD
                     IEATLANEPKQGNWLLLFQKPLLPVLMLGTILFCLQQLSGINVVIYFAPEIFKNLGLG
                     STTGQILATMGIGLVNLLVTIIAILYVDKLGRRKLLLLGFAGTSLSLFALSLFSLNHV
                     AWLSYLSVICLMVYIFSFAISVGPIPHIAMAEIFPLHVRGAGMGMSSMSNWSFNTIVI
                     FSFPVLHQMFGIEVTFVLYAVICFLGFIYAYIYMPETRNISLEQIETYIMSGKPLRFL
                     GREDEEVNAVSTKSESSLLASTN"
     gene            complement(13784..14446)
                     /locus_tag="VAH76_000847"
     CDS             complement(13784..14446)
                     /locus_tag="VAH76_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO1385494.1"
                     /translation="MSFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIH
                     VLEVTLRTPVAIKALELLINTFPDELIGAGTVITPGQLHDVAAAGARFAISPGQTREL
                     LIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKF
                     CPTGGINSKNYEEYLCLPNVVCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCS
                     QN"
     gene            complement(14433..15440)
                     /gene="glk"
                     /locus_tag="VAH76_000848"
     CDS             complement(14433..15440)
                     /gene="glk"
                     /locus_tag="VAH76_000848"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEO1385495.1"
                     /translation="MNFFMMPHDLAQYAIVADIGGTFARFSRVNLVNLQMDKIEISPC
                     AEFISLESALLTYKNRHSLQEIKHIAIAIACPVIDDLVSMTNCHWQFSITELKQRLGL
                     EVLEVMNDFTAIAMSLPVLSTQDLVQIGNGYLDASKVRVVLGAGTGLGVAYLIPHQHH
                     YSAFAGEGGHADWGAKTEQEWFIYRYLKSKYSHVSYERLLSGQGLENLYQALAAYHSK
                     KVEFLSAAQIISLALNQECFIAHKAVAQFFSSLGSFAGDLALTYGAFGGVYIAGGIMP
                     RLLSLVHQSDFRIQFEDKGRFSDFNALIPTYVIAAAQPGILGASVSLKQILTGASHVL
                     F"
     gene            complement(15415..17253)
                     /locus_tag="VAH76_000849"
     CDS             complement(15415..17253)
                     /locus_tag="VAH76_000849"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEO1385496.1"
                     /translation="MHPVINQVTENIQNRSASRRAAYLNQIDKARTKGPQRSILHCGN
                     LAHGFAACAKQDKAILRGNAKANIAIISAYNDMLSAHQPYAVFPDLIKEAISAAGGVA
                     QFAGGVPAMCDGITQGQPGMELSLLSRDVIALSTAVALSHNMFDGGLLLGICDKIVPG
                     LLMAALAFGHLPFIFVPAGPMPSGISNQEKARIRQLYAAGQVDKNALLDVEAASYHTA
                     GTCTFYGTANSNQLVIELMGLQLPGSSFVNPNTPLRNELTKAAACQILSLTDLNSDYM
                     PIGQMLDVKNIVNGIIGLLASGGSTNHTMHLVAIAAMAGYWVNWDDFAQLSAIIPLIA
                     RIYPNGQADINHFQRAGGMAYFVKTLLEADLLHPDVNTVVGFGLDKYTQQPQLIDNRV
                     VWVNGPDSSSNNDVLTSAADPFKPQGGLQVLSGNLGRAIIKTSSLYDEHYVIEAPAVV
                     CSSQEEFEYLFNQGLLNKNCVVVVRFQGPKACGMPELHQLTPKLGVLMDKGFHVALVT
                     DGRMSGASGKVPAAIHVTPEAVDGGGIAKIETGDMILIDCERGILQLLVSDEELAGRS
                     PVAIEGYSSVLGMGRELFGNLRLHFTGAEQGACSLFKGNEFFYDAS"
     gene            complement(17241..17954)
                     /gene="pgl"
                     /locus_tag="VAH76_000850"
     CDS             complement(17241..17954)
                     /gene="pgl"
                     /locus_tag="VAH76_000850"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEO1385497.1"
                     /translation="MQFHSFRDAKQLIHDMVEKLRTILSDAIKRRGQAFLVVSGGRTP
                     IDLFKELALADLPWEKVVITLADERCVNLFDPHRNELLVREFLLQEKAKKAKFISLFD
                     EKEEPLGHLNEVSSQIDSLPTFDAVVLGMGEDGHTASLFPCSDELSAGLDDKANAVLI
                     VKPRTASFQRVSLSKRRLLNSSTIFLHLVGQRKRVVLEQAMATNNPEILPICAILNAP
                     DANVQVMYAPSDEEKECIL"
     gene            complement(17941..19431)
                     /gene="zwf"
                     /locus_tag="VAH76_000851"
     CDS             complement(17941..19431)
                     /gene="zwf"
                     /locus_tag="VAH76_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEO1385498.1"
                     /translation="MLLKAESKYPCDLILFGTLGDLSCRKLLPALYQLELANLLHEKT
                     RIIGCAREELDFSAYADLIKNKIQQFLFDEIDEAVWLRFIKKLAYCKLDLNQSDDYQK
                     LTDYVNPSERVVISYLAIPPSLYSQTCRSLARLNLTQKPSRVVLEKPIGHDLPSSVAT
                     NEQVACFFSEDQIYRIDHYLGKETVLNLLVLRFANSIFSTNWDNRVIDRVEISVAEEI
                     GVEGRWEYYDKSGQVRDMLQNHLLQILSLVAMEPPLSLSAESIRSEKLKVLKSLRFIN
                     KSNAHDHTVRAQYVANNLANGKKIPGYLEEVGANQASTTETFVAIKAYVDNWRWSGVP
                     FYMLTGKRLPKKQSEVVIYFKPQPYNIFHPLNEELYPNQLIIRLQPDEGVEVRIMNKI
                     PGLGERMMLHDSKLDLNFHHSFKTQRIADAYERLLLEVMLGNQYLFVSREEIEQAWQW
                     IDSIVSAWEEDNIPLYTYPACTWGPKEVIRLFNGQAHVWGDHNAVS"
     gene            19749..19994
                     /locus_tag="VAH76_000852"
     CDS             19749..19994
                     /locus_tag="VAH76_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385499.1"
                     /translation="MLNKRFSERLNKELDNIGVPEATAERIEILSKLIKIPKFKAEAL
                     LNGITIPDEAVLKTLAQELEVNVDWLIGKSDSKSGAH"
     gene            complement(19998..20906)
                     /gene="rimK"
                     /locus_tag="VAH76_000853"
     CDS             complement(19998..20906)
                     /gene="rimK"
                     /locus_tag="VAH76_000853"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEO1385500.1"
                     /translation="MKIAILATNPHLYSHKRLKAEAEAAGHEVKIINPLYCYMNVAAS
                     NPKVHYRGGAPLPHFDAVIPRIGASITYYGTAVLRHMETMGMYTLNESIAISRSRDKF
                     RSLQLLARKGIPMPLTSFAQSPDDTEDLIHMVGGAPLVIKLLEGTQGKGVILADSHQS
                     AVSIINAFKEMHANILVQEFIEESRGTDIRCFVIGEKVVAAVKRQAKDGEFRANVHQG
                     GKAVKVKLSPQERAIAVSAAKTMGLRVAGVDLIRSNHGPLVLEINSSPGLEGIEKATN
                     INLAGKIIEYIEKKAKPISSNHRFHG"
     gene            complement(21272..21913)
                     /locus_tag="VAH76_000854"
     CDS             complement(21272..21913)
                     /locus_tag="VAH76_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEO1385501.1"
                     /translation="MNIKIKGGAVTITVLALVSLLAGIYISQNFHFKKKINVADFHGT
                     YLENPRTVNNFSLTGTDQKAFNNSSLQGRWTLVFFGFTNCGYLCPTTMAELGKMYRIL
                     EKKGVKNLPQVVMISIDPQRDSLDKLGSYVTSFHKSFYGARGEEDSIKSMTHEMGIAY
                     AKINNKESSEPENYDIQHSGAVMLFNPQGELNAFFTTPHHADLLAKDYVLLVS"
     gene            complement(21923..22807)
                     /locus_tag="VAH76_000855"
     CDS             complement(21923..22807)
                     /locus_tag="VAH76_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEO1385502.1"
                     /translation="MRTEYAARLPVDWRDYVELCKPRVVLLMLLTVIVGMYLAAPGWV
                     SLRLIAFTLLGIGLCAGSAAAINHLVDRHIDSIMARTKKRPVAYGRVSVKQALWFAVI
                     IGLMGLSLLILFVNQLTALLTFVTLIGYAGVYTGYLKRATSQNIVIGGLAGAAPPLLG
                     WTAVTDQLDPQALLLVLIIFTWTPPHFWALAIYRYKEYQDAEIPMLPVTHGIQFTKLN
                     IYLYTVLLLVVSLLPFVVSMSGWIYLLGALVLGIRFLVWAHKLYFTDKPVVAMQTFRF
                     SILYLMLLFVFLLVDHYF"
     gene            complement(22797..23804)
                     /locus_tag="VAH76_000856"
     CDS             complement(22797..23804)
                     /locus_tag="VAH76_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEO1385503.1"
                     /translation="MSNSSIRLAATLAVFLAFFVVMLGAYTRLTDAGLGCPDWPGCYG
                     RMVLPGADKGLQEAQSQYPDLPIESKKAWTEMAHRYAAGTLALLIFFIAFQVWFKRRQ
                     DQDLPWHLPLALFMLVVFQAALGMWTVTLKLLPVVVMGHLLGGMLIVACLCRFRLQIA
                     QLKGQNLPEWRPWLRLGIVIILLQIALGGWVSANYAGISCIGFPLCNGTWFPELHFAK
                     GFNLFSTIGANYQGGVLESEVRASIQFIHRIGAILTATYLLALSLIIVFKTHSIYLRA
                     TAVIMAMLVLLQFTLGIMNVIHLLPLKVAVAHNGVAALLLAVAFCSLHFTQDREVSHA
                     Y"
     gene            complement(23809..24354)
                     /locus_tag="VAH76_000857"
     CDS             complement(23809..24354)
                     /locus_tag="VAH76_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385504.1"
                     /translation="MKKPYKYMTLLLLVVIFAAPGITAYLFYKNPSWLGTSKVNKGIL
                     LSPPIALKPLDSSAKWRIIYWNPNGCDKTCLKQLNMLGRVRLSFGRKLYQVDEWLLLS
                     NKDSLIAEELKPILKEQDIHVAALSLTEMSKLSTLYPEAKIFIANPDNYLILSYQPGC
                     NPGDIYKDLKLLLNTTEIKSG"
     gene            complement(24338..25066)
                     /locus_tag="VAH76_000858"
     CDS             complement(24338..25066)
                     /locus_tag="VAH76_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEO1385505.1"
                     /translation="MPSLTCFNFRFTSNWPMLILTAVCFFLFISLGFWQIHRADEKTE
                     MITAQQALAKKEPIIWQPGQKLPEQYQRISIEGAFLPKLFLLDNQHYQHQFGYDVVSP
                     MLLDDGSIVMVDRGWVSGDITRRTFPNVQTPNGKFKLFGMVYFPSKKQWILGPSFEEK
                     ENKVTLLELIDEEILKQLLQKKVYPFIIRLDKNEPFGFVREWEIVSMPPQRHFAYAIQ
                     WFAMALVILIIFVIFNLKKNEKTI"
     gene            25134..25337
                     /locus_tag="VAH76_000859"
     CDS             25134..25337
                     /locus_tag="VAH76_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEO1385506.1"
                     /translation="MFTKIFIIFVMILIVASLASGLFYLVKDTDKSKRTVKALTVRIA
                     ISLSLFVFLFIAFKLGLIQPHGI"
     gene            25754..26302
                     /locus_tag="VAH76_000860"
     CDS             25754..26302
                     /locus_tag="VAH76_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEO1385507.1"
                     /translation="MNKQMQWHLFGNTSGLLIICCLLILAFLDQFIFYELPCPICILQ
                     RICFVAVGLCFVMNLMLGIRASHYGLMLLASLLGLSISVRQMYIHLTPGDPGYGGLFL
                     GLHLYTWSAIIFLVIILLIAGALILDKGFMPDYKVRSKSAKVMIWVFFILILANGIST
                     FLECGPHECPDNPSKYYLLNNN"
     gene            26321..26764
                     /locus_tag="VAH76_000861"
     CDS             26321..26764
                     /locus_tag="VAH76_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEO1385508.1"
                     /translation="MRLLFISLILASGYLQADTINNYMNIANNIPQMEMKADPQAQAW
                     ARSARHVLTITSESIAETLIQANEAAKTQGKPIFCLPQGVQLNSFVMNELIQQTYKEI
                     SSQQSDKDKMTVSQVALLGLSKKYPCEPSPQEKQIQHVASLLSPQ"
     gene            complement(26978..27319)
                     /locus_tag="VAH76_000862"
     CDS             complement(26978..27319)
                     /locus_tag="VAH76_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO1385509.1"
                     /translation="MSDKFSHITKDITTQLAKFRKEMPELMTGFSSLAQAATKDGALD
                     KKTKELIAMALAVAKQCPGCIGFHSQTLVKLQATREELLETLGMAVYMGGGPSLMYAA
                     EALEAFEEFSK"
     gene            complement(27523..28113)
                     /locus_tag="VAH76_000863"
     CDS             complement(27523..28113)
                     /locus_tag="VAH76_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0405 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385510.1"
                     /translation="MLYNLARQVNFNNPSCLCNFQKMGYIDELLEELSARLPELEWKI
                     NGLSSSISIHNLPKGIFSSVIEFNGPACIKEIRDDIHALQKHKDESIACFLAERIQKK
                     INVLVILCQMDKKNNKPESKISFDLTTLSTRQQWIKTMEENICALEEQHQAMRKTLEQ
                     MKSCSNPATILHLQTELGNVEKRLTLAKETLNRAIS"
     gene            complement(28103..29506)
                     /locus_tag="VAH76_000864"
     CDS             complement(28103..29506)
                     /locus_tag="VAH76_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1385511.1"
                     /translation="MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILG
                     ALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILS
                     FVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
                     MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHAN
                     DVKDPQHAFPKALVYSVGIILSTLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYH
                     MDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLY
                     TQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRP
                     FKIPGGMAGMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPP
                     FITSWLQAKEADLEPAV"
BASE COUNT         9556 a         6188 c         5177 g         8647 t
ORIGIN      
        1 agattcagcc agtttgattt ttagaatact atggcgatag tttaaaatag gtgattacaa
       61 tgggtagaag tgaaatgaaa atagcatcag ctaaattgcg tgaattgatg aaggcagtat
      121 ctgaaggtca ttatgaaact gtcaatacaa tactggataa agatcctgag cttgtaaatc
      181 agtacgtccc ccccacttac gattcacccc tggcaagagt attaaataaa aaatacattg
      241 actacaaaat gcttgatatt ttggttaagc accatgttga ttttgattat cccatcaacc
      301 atcacaaaga aacaccaatt gaattggctt gtaaaaatca ggatcttcaa ttattcaaat
      361 acttagtgca acacaacgcc cccatttcag agcatgcacc acatttcctg ctggtgaata
      421 gcaccaatat caaatacctg acagaagata aaattaaaaa tacctgtgaa attattaaat
      481 taatgggtgg tttggaagcc gtctcttcta aatgtaatgc ggaaggtaat cgcttcgagg
      541 aactggcaag gaaatcacaa ttaatcaatc gtttcggtgg tgtagtaagg tacgattaca
      601 tgcaattgct gcaatcggtt tatccaattg ttgatagaga agtggatact ccaaccatta
      661 atgcctctac tgaaaaactt acaaacctgc tcaataaaat tagggatcag cacatctcaa
      721 aagaaggata cgatcaacaa aatttaaaag attctatcag ccttttcttt atgaccggtg
      781 gagaaatacc tccttcaaga aaagttccag agagtcgttt tgaagaagct ggcatagaca
      841 cccctaaaaa cacgctttaa atttttttat cctatctatt aagtcgtcaa gaagcaccaa
      901 atttactatt tggcgtttct tggcaccccc gcatctctcc tttatataac tctcaactca
      961 taagaacttg attgcgcctt ctaagccaat acatacgatt ttatgattac ccatttatgc
     1021 aatttttttt gctacaataa aaaatttatg ccaatgctta taataaccta aagcatccat
     1081 ttcatccttt tcttcttctt gaaaatattc aattttgaat tgagaaaaca aatggcaaat
     1141 catttcccga tttaaaaaag tcatatccgt tctggtattc cagaagtcat taaaaccaaa
     1201 aaacgaacca gaaaatcgac catctggctt aagccctttt aaaataatgt tccagaattt
     1261 ataaaagtaa actggcttga gaaatggaag gctaaagctg gcgttaatca agtttacttt
     1321 agggagtgaa tccaaagcct caaatgaaga aactattgtt tttagtttgt tcgcgcatgt
     1381 taattcttga gctgaagaaa gcatactgtc tatagcagat aattgtgaat caatggcaat
     1441 cacagaccaa ccgcatcgca aaagttccat aacatcgata ccggcaccac atcccaaatc
     1501 gattgccgat ttagatagat tgatcttttc cttctcaaaa tttgcaattg ctttaagcaa
     1561 agactttctg gggagagtgt tttgtttcgt agaattataa taagccgtcc aatttctttt
     1621 attcatttac tatataccct ctagttacca aaaggtaact aattgactga ttttattaaa
     1681 tattttagtc tcgaacctgt tcattttata aaataaagga gtggattatg aaagaaaaac
     1741 tcattaaaaa aattgtccag aaaaaactgc taaaaaggaa tagaggatgg tgtggtccag
     1801 gaacttgtat tggatgatat aactgcaatg atggcacgtt atcaaaaaac gtgccgatac
     1861 acttaaacca atcaaagccc cataatcatg catcctgact taaaagactt acttgattac
     1921 agaaatcata aaattattac cagatataat atggattacc cgactgcagc catgcaagct
     1981 gaagaggcat tacatgaatt aatgaaattt atttggctct gccataaaca taaagcagat
     2041 aaattacttt atcctgatga taaaaatctg gatttctctt gtgtcattca tgctgaaatg
     2101 gctgatattg acaagatgtg gcatactttt cttttattca ccagagatta tcatgagttt
     2161 tgtgatacct gtctcaatgg aatttttttt catcatgacc ctttaattgg ggaaaatatc
     2221 cctgtttctg aagaggatta tgctctggag ttgacccgct atttaaccta tatccatgac
     2281 catttaggtt cggaaacttt aatcaaatgg tttcaataaa tcaggcttga caaccaagct
     2341 tctgttcgca aaatgttttt tccgctttga ttattttatc catattggtt tcagtccaac
     2401 gagtaagtaa aaccaatatg gcacttaatt ccttacccaa tctggttaat gaatactcaa
     2461 ccttcatagg taaaacagga taagattgtc tttggacaaa gccatatcgc tccaatttca
     2521 gtaatgtttg cgtcaacatt ttaggtgata ttccaccgat ttcacgtttt aactctccaa
     2581 aacggtatat tttttgggat aaaaggctaa taaccaatat agaccattta tcactaatac
     2641 cttcaagcac attatgcgat gggcacttct cgttataaac actataatca ggatgaggca
     2701 tatttgtact ccctatttct aattaataac gaccttcaaa ctgaataaaa tgggttcgag
     2761 ctttattcat cagtaactta aatcgttgta aatgctgttc agatgccgtt gaggaatgta
     2821 aaacaaactc tgaaatacat aatgcaaaaa ccaagcgtcg tatttcccaa atcgtttctt
     2881 gaaaaatatc aattcttttt ttagtatgtt cacttgtttt aaaatccagt ttcaaatttt
     2941 cggagctgat ggatatcggg agataatgct cggtaaattg accacgtaat ttctctacaa
     3001 agagcaatgt attaattttt tctgtattgg attcgtctat ttttcgcaca atatcattta
     3061 aaaaatcata aaccatcacc tgatgaaaaa aataaagtct atgagaataa cggtatgcct
     3121 gaaataaaaa ataatcgcct gccaacacaa taaaaacacc aaacagtgta cagatgcctc
     3181 gattaagaac catctcaaaa ggaccacgag gcgatgtagc ctcttccgtt tgcgccatca
     3241 aaaaaaagac aactaaagta ataaaaaata cactaatgtc gtaacgtctt gtgtttaagg
     3301 tagcaacact caaaccaact atcgccagaa caaataagat tgatatcaat cgataattca
     3361 attgcaataa atagagtaag ggaatcaaaa tgatgagagc taacagtgtc cctcctatcc
     3421 tgtgtttagc tcttctgata atcaatcctg gttcaatacc cgcgcttact gacaagacag
     3481 tgatcaatac ccaccatttt tcaggaaaag tcgaaaataa aaaaatgagc atggcaacca
     3541 tgaacatgat gactagatga ataaatcgag cataatcaag ttttttcttc aatgctgaag
     3601 tatcatgcat aatcgattcc agtggctaaa tgcttgttgc aaacatctca taacatattg
     3661 ttgtaatttt gactcaggca cgatagacat tttaggagtg tcacaggatg tgttggtttt
     3721 aatcgcacat ctaagagtat aaagattctc tttcactcca tgccagaaag cctcattttt
     3781 ggcttcataa taaagattat ccaaagaaaa ttgaatgttg cgtatgttga ttgaaattcg
     3841 ctgagtataa atcatgtatt ttttggggat aagcctacga tattgcctaa gcatacccaa
     3901 atgagtcatt tcttcctgca taaatctggg gccgcggtgg ctaaaagatc cttcaatatc
     3961 ctgttcaagg gattgaatga attttgccaa agccctcttc catacactga gatattgatt
     4021 ggggaagaat cttaaacaaa tgaatatggc gatcatagat aaagcgcttg acccaacgat
     4081 gtcgtagaca atttgccaat ttgcaggagg ttctgtactt aaaattgtcg ctccggaagc
     4141 aataatgagc atagtaacat tccttaatgg aagataattt tttagcacat aaaaataaac
     4201 gcttgctaag gcaataattg agaaaaagaa aaatacaacc cgaaatgggt aaacaagata
     4261 aaatgaggta ctcaacaata ccactaccac tccaataaaa atcaaaaagt gttctttctc
     4321 tttaaagctg gtaagactgg gtgattcata aaatcttacg agcaaaaatg gtgaaaaaaa
     4381 tgccgtaaaa ttcgtgggac gaaataccca ataaacatag gccattacgg tggcaataaa
     4441 aagtgattta tagagcgcta ttcgttgaac agcataagga tcaaccttat caatccattc
     4501 aataaacttc gacatagaca taagcgctag ctccaacacg aagcgggtat ttagggtcag
     4561 ggtcagtaac ctttataatc acaggaaggc gctggggcaa taaaatccat tgattttcat
     4621 tgataacatt ttgcagctga gtacgatcat ccaccaattg cctattcgcg gaccaataat
     4681 ccgaatcaat aacaccatga aatatttttc ttcctaaata cattcttgga aatatcaata
     4741 cctttgagcc cttgcgcaca tcacgcaaat ccgtctcgtt aaaattggcc tgaatataaa
     4801 tattatcctg atcaaccaat gagaataatg gctgattgat attgactgga gttcctatgg
     4861 taaaaaacaa attttgaata actccattac cctgagcata aacatccgtt aaatccaaat
     4921 tgactttggc attttttaac cgggcctgaa tggattttat ttcattttcc tgaactgtta
     4981 tttgatgctg atctatttca agttgtttta atgaagcctg ccatttatct ttagctgctt
     5041 ttgtttcttg ctgggagttt tgcagggtaa tcagagaaac agattttata gcataccctt
     5101 taaggtactt ttgatcatct tgcgccagct ttttatatat tttttgctca ttttcgctaa
     5161 gcttcaaatc ccgttcgtac gtagctttta atactgccaa ctttgcctgt gctccagcta
     5221 aatcagcact gagctgttca actgtgtatt catagggctt tttgaatacg gtaaatagtt
     5281 tttgtccctt ccgcaccgca tcaccatttt ttacataaag ctcggttata tatccgcttg
     5341 ttaatgccgc aaccggacgt acattgttta ctacaaacgc gttatcagta aaagggatca
     5401 aataggaaaa aacatagata attgcagtaa gaaaaccgat aaaaataatg acgttagtaa
     5461 gagtaagcca gcgttttggt tttaaacgaa gcaagtactc ttttattttt ccaccatatt
     5521 ccatcaattt tttcataaaa tagccaacca gtttcccttg gtatataaca ataatgcgaa
     5581 attagcttat ctcatcgaac agctcaagag acaagaactg caattttagc ccgcctggcg
     5641 acttttaaaa tcagtgcgtt acaaaccaaa ccgatttagt cactacaggc atacccacca
     5701 gccaaatctt gatacaacat aaccagtgac atggccaatt ccagcttagc ctgatttgtt
     5761 gagagcgcca gggtatccag ataaacctta ctctccaata actctttata ggacattaac
     5821 cctgatttta ataaaccatt ttgtaatttg tatttatgct gataatcttc ttgtgcgtgc
     5881 cgataagata aaaattgctc tctcatgcgc ttattagctg aaaaagcatt atcaacttct
     5941 tttaaaatta atcggattgt cttaacgtat tcggccaatt ttgcattata aactcccttg
     6001 ctaactgaaa ttttacccag agtattggga ttaattgtcc agtttacgta cgcatcggtc
     6061 gcttgttcaa acgtgctatc aggcaaatgt gcctctccaa taaaatcatc cagctgcata
     6121 acaggaaaaa aatcactata agcaacaaaa atacctgcac gagccgcttt tagggaatac
     6181 tccgccatct tcatatcagg gcgattactt aatacgctag cgggtaaact tcctggttta
     6241 aagcgggtaa aatccaattg agcaaaatta tttttgcttt tgattaaacc aggattctca
     6301 tttattaaat aacgcaacgc attctgacta acgacaatgt tgtgtttaat gggttttatt
     6361 tgtgctgcaa tcaagcgctc gttggattgc aactgagcca aatcaatatc attttttaat
     6421 cctatgttga tttctttcct gctcaaagca attaacgact ttaaatccct atccagttta
     6481 tacaataatt taagttgctc caactgtgca atcaaagtga aataagccgc tgccacctga
     6541 ccaatcaagg tcaaacgcaa accatcgatt gctgcctgat gatattggac ctgatattga
     6601 gcacgctttt gctgagtata aagttgcata atattcaaaa tataacttgg ccatattcca
     6661 taaaaccctc ctggcacacc aagagcagga ttagttgaat accctcccaa tatttgaaca
     6721 ttaggtatcc aactcaactt cacttgctgt aactctcctt gagcttgctg taaattagcc
     6781 attgcaatat gaatatccat attggtcttc aatccagtat caattaaacg atccaattct
     6841 ggatcctgaa attgcttcca ccaggccaaa taaggcaact cctgaactgg tcgataatcc
     6901 ctgggactgg aggggaattg cttcggcgtt ataatattgg aaatttgctg atcagtgctt
     6961 tgaccacagc ttaacaaacc tgctcccaac aataataaga acattaattt tttaagcatg
     7021 cacatcatcg tttatttcaa agtgaatgtt tatcattgta atttataatt catctaatgg
     7081 tgaaagtata tttaaatttc aacaaggagc tctcatgatc aaaaaaatag catttaccat
     7141 gtatccagtt caaaatatgg atagagcaag gcaattttat gaaaaaacac ttggaattaa
     7201 agtcggtaaa atttccgctg agggagcatg ggtagaatat gatttacctc aagggggttg
     7261 ttttgcaatt actacattgg ctcaaggagt tatccccagc gctaattccg gaggtagcgt
     7321 tgcttttgaa gtagataatc ttgatgagct ggtattccaa ctaaaatcca aaggagttca
     7381 gttcaaaatt gacctattcg cttcacctgt ttgcagaatg gcgattgctg tggattcaga
     7441 gggaaatgcg ttcactttgc atcaattaac atgattgttg ctgccaatac gtgtgagaat
     7501 ccctaaagag ggcctcacac gtattggtgt tatgagaaac atcaatattt ccaaaaatac
     7561 aaaataacaa tgttccttag tatgccttgt ttactaacag gacttacgat gaacctctag
     7621 gtcgaggcaa aaaatgtttt taatgaggga gtttagataa actaaatgac cgagttaaaa
     7681 acatttttta acaaagagat tggggtttat cgtaagccct gactaaatca cccaaggtag
     7741 gcagcagact ctttagttca ctttcaggat aaggaataga aggcatggaa gattgccaaa
     7801 taccttgagg ccacaaagga tcatttttgc tgcgaataac aatatgtata tgcaattgcg
     7861 gaacaatatt gccaagagcg cctatattaa gcttgtctgg tttaaaataa tctctcacca
     7921 gaagagacag ttgattgatt tcttcaatta ataaaaatcg ctcgctcttt tcaagctgat
     7981 aaatttcttg aatatccatt tttcttggaa ccaaaataaa ccaggggtat gaggcgtcat
     8041 tttttaaaag aaccgtagat aaaggccact ctccaagtat aaaagaagac gacttaatcc
     8101 gatcatcaat gcaaaacaaa atactctctc tatcaataag tcctcaatca ttataacaaa
     8161 aacaagacct tcagattata agaattacag ataaatttta ataaacctat tgagtaatga
     8221 tccctttttg cgttatgttg aaattaccta ttgtaaaata ggctattaac aattgtcatt
     8281 taaggaagaa tgcaattgct cagctgaccg cttatgtcag tcgacgattg ataagcttta
     8341 gcataaggag gattcatggc tagaggcgaa gtcaagtggt ttaataacgc caagggttgg
     8401 ggttttatta tcccggaagg tggtggtgaa gatatttttg ttcacttctc ctcaattcac
     8461 ggcacgggat acaaaacgct ggttccagga caggcggtca cctatgatgt cgttaatggt
     8521 gaaagaggtt tacatgcctc taatgtcgtt gctcttagtg aaaacgccga acaagaagaa
     8581 atagcttaac ccaattaatg tgataaacta ttaagcgcgg cagtataact aatatttatg
     8641 ctgctgcgtc catcaacttg aggtattatg tccttcattt tataactgga ctttagctga
     8701 tgagacgtgg tttattactg ttaaatcttg gcactcccga taatgcggat atcagggcgg
     8761 taaagttata tttgcgtgaa tttcttactg acaaaagagt aatagactta ccaactatcc
     8821 cgcgctatat cttggtatat tgtttgatct taccctttag atcgccaaaa tcagcccagg
     8881 cttaccaatc aatatggact gaaaaaggct ctccactttt gtatcatagc caaaatctag
     8941 tgacaaaatt acagtctgca cttaaggatg agtacaaaat tgctttggga atgcgctatg
     9001 gaactccatc aattacaaca gcacttgcag aattaaaaga ttgccattcc cttaccattt
     9061 tacccttatt tcctcaatat tcttctgctg ctacaggttc agccattgaa aaaaccttat
     9121 cctatttggc aaaccaggaa attattccat caattaaaat cattcgagat ttctaccaaa
     9181 gaccagaata catccaggcg caagctaaaa ttatgaagcc ttatattaaa gacaatttcc
     9241 acgttctttt tagctaccac ggaattcccg aacggcatat tcacaaaagc gggtgcgata
     9301 cgctttgccc ccaaacatgc acacccatat acgataaaaa tcaagcatgt tatagggctc
     9361 aatgctatca gaccagtcta ttactggcaa aagaattgca attaggcacc caccaataca
     9421 caaccgcctt ccaatccaga ctcgggaaaa ctccatggat aaaaccttat accgatgaaa
     9481 tttttgccga actaataagc aaaggaatta aaaacattgt ggtctcctgc ccatcttttg
     9541 tcgcagattg cctggaaact cttgaagaaa taggaattcg cgcaaaagaa cagtgggaaa
     9601 aacttggcgg tgagcaattt atattaactc cttgcatgaa cgatcatcct gagtggatca
     9661 aagcaattca cagtattgtt aacgagcaaa tttaacctgc cgtcttgctg atttaaccat
     9721 cggcagtcgc aacaaaatct cgttttacat taaatattta ttgcaaaaca ataaatattt
     9781 aataaaatac gcatgatctc atcttagaag gacaattata taatgaaaaa aataaaatct
     9841 gtgagtcatt tgctttttct attattcaaa acattagcct atggccttcc ccttgtaacg
     9901 acttatttca ttctatttca tttcgagagc atgctgaatt ggggaggatg gcactccctg
     9961 ttatcaaccc cagtgaaaga cccctctcat ttttccttgt tgcatcgttt cattatttta
    10021 ttgattgaag cattaccctt atccattaat gttctaattt gtcatcaatt ggcaaagctg
    10081 tttcgcttgt atgaacatgg ctatttattt gaagaagaaa acattcgact gattaagaat
    10141 atcagtattt acctgatttc aggtgaactt atacaattgc tttatcaacc tttgataacc
    10201 gcagctctga cttttaccaa tccatctggt caacgtatcg caagcatcac cttgggaaca
    10261 gcgaacctgt ccacgttaat taccgctttt atcattttga ttgcctcttg gattattaag
    10321 gaagcacata aattaaaaac ggatgccttg ttaaccattt aatgtgaact tcagcgtaag
    10381 gacatcaaga gattttctta cgcagtctca atcagtttat atcccgcttg tttaaggagt
    10441 ttcaatcgat gtcaattatt gttaatttgg acgtgatgat ggcaaaaaga aagtgtcgac
    10501 ttagagattt agctgaagct ataggcatta ctgaagctaa cttatccatt ttaaaaaatg
    10561 gtaaagccaa agcaatacgt tttgcaaccc ttgaggctat ttgtgcttac ttgcattgtc
    10621 aacccggaga tcttcttgag tatcaatcca ctgaagacat caccttatca aagacagggc
    10681 ttaatctgag aaagctttga gtaaggactt gcaaaaaacc agggtcaagg caaaaagtaa
    10741 ttgatgttaa acacatttta caataatccg ccctaaagac tatggtctga aaaactcatt
    10801 tcctgtacta tggcgacctt cttttttaaa actaactact atcaggagat tatgttgaaa
    10861 aggatctttt tcttaatgat tttttttgtc tctcaaacta tggcttcagt atttacccat
    10921 gaagaggttc aaatattaaa aaaacatttt ttaaataact ttcaaaccaa tggagccatc
    10981 gtagcctcac cctcgcaata caatcccaat tattattatg actggattcg agactctgcc
    11041 attgccatgg gattggttga gacatggtat gaagccagcc aatctgctcg atataaaaaa
    11101 cttttattag aatacgtttc atggacagaa aaaatacagc atcaagcgga tcccattgct
    11161 ggccaggata tattgggtga acccaaattt tatatcaatg gcaatccttt tgatggggaa
    11221 tggggacgac cacaaaatga tggtccggca ctcagagcat ctgttttaat tcgttttgcg
    11281 caacagcttc tcgatcataa tgaaatagat tatgtcaaat cacatcttta taacaacacc
    11341 atggacccac aatccatggg cactatcaag atggatttgg aatacatcgc gcatcattgg
    11401 caagatgcaa attttgacct gtgggaagag gtttatggcc atcatttttt cacggctatg
    11461 gcacaacaaa aagcgctgac cgatggcgcc attttggctc gccaacttca tgacagacaa
    11521 gctgctgttt tttatgaaat gcaagccaac ttaattaaca gtcgactgca acagcatttg
    11581 gaccatcaga ataaaataat tcaagccaca ttattacctc accctggtcc tcaaaaaaca
    11641 ctagagcttg attcgtccgt catgttgggt attttaacta atccacaaaa agaaggtgtt
    11701 tttgccccac atcacacctt tgtgcgaaat actgcaaagg ctttgcatga gcaatttaat
    11761 ttgatgtttc ctattaataa aaaccgttct ggagcaatat tgtttggtcg ttatcctggc
    11821 gatacttatg atggctatca aacaaacagc ataggaaacc cctggtttat cttaacagct
    11881 acgatggctg aatattattt taccatggct cataatctgt ccctgaacag cagtaataaa
    11941 cttcatatac agaactatct aaagaaaggg gacaattacc taaggctgat aaaacaatat
    12001 ggccctgatc tgaatttaag tgaacaaatt aatttgaata ccggagttca gcaaggagcg
    12061 acatccttga cctggagtta cgtttccgtc ttgcgagcta ttcatttaag agagcaattg
    12121 gaaaatagaa taaaaactat gggctggaat acctattagg attttatacg atcaggtaat
    12181 gcgataaaga atagaatttg tggcccgtgt cagtcaaaga taacacgggc atcagaggaa
    12241 aatttgtatc aatttgttga agccagcaaa gaagattccg atttagttga cacagcatta
    12301 acctcttcat cctctctacc taaaaaccgt aatggcttgc cactcattat ataagtttct
    12361 atctgctcta aactaatatt cctggtttca ggcatataaa tataagcata aatgaacccc
    12421 aggaaacaga ttaccgcata cagaacgaaa gtcacttcta taccaaacat ttgatgcaga
    12481 acaggaaaac taaaaataac tatagtatta aatgaccaat tactcatgga tgacatcccc
    12541 ataccggcac cacggacatg aagaggaaaa atttctgcca tagcaatgtg cgggataggg
    12601 cccacgctga ttgcaaaaga gaaaatataa accattaagc aaataacgga taaataagat
    12661 agccaggcaa catgatttaa tgaaaacaac gataatgcaa acaaactcaa actggttcct
    12721 gcaaaaccca gtaacagtaa tttgcgtcgc cctagcttat cgacatacaa aatggcaata
    12781 atagtaacca gtaaattaac taacccaatc cccattgttg ctaatatttg tccagtcgtg
    12841 ctgcccaagc ctaaattctt gaatatttct ggtgcaaaat aaattaccac attgataccg
    12901 cttaattgtt gtaaacaaaa caatatcgtt cctaacatca acacaggtaa caagggcttt
    12961 tggaatagaa gtagccagtt gccttgtttt ggttcgttcg ccagtgtggc ttcaatatct
    13021 tttaactctt gctcaactga ttgtttgcct ctcaattttc ttaaggagtt tgctgctgca
    13081 tcatgacgtc caacgctgca taaccacctg ggagactccg gcataaacaa aatccccagg
    13141 gtcaataaca aggcagggat agcacttgaa gcaaacattg cacgccacgc ttgctgctct
    13201 atcaataaat aattgaccga ataagaacaa acaataccca cagtcaatgc aagttgataa
    13261 attgcaacca ctgcaccacg tgattgcata gcggctgttt cagccaagta taatggagtt
    13321 aaaactgaag ccattccaat ggccaaacct aaaattaacc gagataaaat cagcactgaa
    13381 atggtttcag caaaaccggc acccagggct ccaacaaaga acaaaaaacc ggcaaaggat
    13441 aataaagaac gtctgccgaa acgttttacg cactttgatg ccatgaatgc accaatcaac
    13501 agcgaaccaa acaatgcccc aaaaggtaaa gcagaagcca taacaccaat gtgggttgca
    13561 ttcagattaa aatgattttt taccagtcca agagaacctg ctattattcc ctcatcgtaa
    13621 ccaaataaaa aaccggcaat tgacccaatt attgcgacaa tccaagccat ttttcactcc
    13681 ttgattttaa attcttttcc tctgtctcta ctctacacaa tgaactgttt tagctggcac
    13741 tatcccttac atcacgtgaa aacatttttt caactcgaat agattaattc tgcgagcaaa
    13801 gttctcgctt ctgagaactc accgccatac ataactctgt aattaaagac cagttatgat
    13861 tcttgatagc ctcttccggt acaatccagg accctccaac gcaaactaca tttggcaagc
    13921 aaagatactc ctcataattt ttggaattaa ttccgcctgt tggacaaaat ttaacttgag
    13981 ggaatacccc cgatatcgct tgcagcatag ggatacctcc agccgctgcc gcgggaaaaa
    14041 atttaaaatg gttatatccc atccccaagc cctccattaa ttcagaaaca ctggctacac
    14101 ctggaatgag aggaatttca cttttctgtc ctgctattaa taattccctt gtttggccag
    14161 ggctaatggc aaatcgagcg ccagctgctg ccacatcatg aagttgaccc ggggtaatca
    14221 cagttccggc accgattaat tcatctggaa atgtattgat caataattcc agtgctttta
    14281 ttgccactgg tgttcttaat gtcacttcaa gaacatgaat ccccccggca aataaagcct
    14341 cggccaaagg taacacatcc tctaactctt tgattacaat gactggaata accggagatg
    14401 cagcaaatac agcgcctggt tgtgttttcc attcagaaaa ggacatgaga agctcctgtc
    14461 aatatttgtt ttaaactgac gctcgcgcct aaaatacctg gttgagcggc tgcaatgaca
    14521 taagtgggaa ttaaggcatt aaaatcacta aatcgtcctt tgtcttcaaa ttggattcta
    14581 aagtcgcttt ggtgtaccaa ggagagcaaa cgcggcataa tccctcctgc aatataaaca
    14641 ccaccaaaag ctccataagt taatgctaaa tcaccagcaa acgatcccaa acttgaaaaa
    14701 aattgggcta ctgccttatg agctatgaaa cattcctgat ttaatgccaa agatataatt
    14761 tgcgctgctg acaaaaattc aactttttta ctatgatacg cggctaaagc ttgatataaa
    14821 ttttccagtc cttgaccaga cagcaaacgc tcataagata catgggagta tttacttttt
    14881 aaataacgat agataaacca ttcctgctca gtttttgctc cccaatcagc atgcccccct
    14941 tcccctgcaa aagcactata gtgatgttga tgaggaatta aataagcaac acccaaaccg
    15001 gttcctgcgc ctaacaccac tctcacttta ctggcatcca ggtaaccatt accaatctgc
    15061 accagatcct gagtcgataa tacaggtaaa ctcatcgcaa tcgcagtaaa atcattcatg
    15121 acttccagaa cttcaaggcc aagtctctgt tttaactctg ttattgaaaa ttgccaatga
    15181 caattcgtca tagaaaccaa atcatcgatg actggacacg caatcgctat cgcaatatgt
    15241 tttatttctt gcaaagaatg ccggttttta taagttaata gcgcggactc aagactaata
    15301 aactcggcac aaggagaaat ttcaatttta tccatctgca aattgacaag attaactcta
    15361 ctgaaacgag caaaagtacc gcctatgtcc gcaacaatcg catactgcgc taaatcatga
    15421 ggcatcataa aaaaattcat ttcctttaaa tagactacat gccccctgtt ctgcaccagt
    15481 aaaatgtaat cttaaattac caaacaattc tcgtcccatg cctagaacag aggaataccc
    15541 ttcgatagca actggagagc ggcctgctaa ctcttcatca gaaactaata attgaagtat
    15601 tcctctttca caatcgatca aaatcatatc gcccgtttca attttagcaa tcccgccccc
    15661 atcaaccgct tccggagtaa cgtgaattgc agcgggaacc ttgcccgatg cgccagacat
    15721 tcgaccatca gtaaccaatg ccacatgaaa ccctttatcc atcaacacac caagttttgg
    15781 cgttaattga tgtaattctg gcattccaca tgcttttggg ccttgaaaac gaactaccac
    15841 cacacaattt ttattaagca aaccttgatt gaatagatat tcaaattcct cttggcttga
    15901 gcaaacgact gcgggagctt ctatcacata atgctcatca taaagactgg acgtcttaat
    15961 aattgctcta ccaagattgc cacttaaaac ctgcaaacca ccctgaggtt taaatggatc
    16021 ggcagcagag gttaatacgt cattattgct tgagctgtca ggaccattaa cccaaaccac
    16081 tcgattgtca atcaattgcg gctgctgcgt atatttatcc agtccaaaac ccacgactgt
    16141 gttgacatca ggatgaagta aatcggcctc tagcaaggtt ttaacaaaat aagccatgcc
    16201 tccagctctt tgaaaatgat tgatgtctgc ttgaccatta ggataaattc gagcgattaa
    16261 tggaataatt gccgacaatt gcgcaaaatc atcccaattt acccaatacc ctgccatagc
    16321 cgctatcgca acgaggtgca tggtatgatt ggtcgatccc cctgaagcca ataatccgat
    16381 aataccgttt acaatatttt tcacatcaag catctgccct attggcatat aatcagaatt
    16441 taaatctgtt aatgacaaaa tctgacatgc tgctgctttg gttaattcat tgcgcaacgg
    16501 ggtattagga ttcacgaagg aagagcctgg gagctgcagc cccatcagtt caataactaa
    16561 ttgatttgaa ttggccgtac cataaaatgt gcatgttcca gccgtatgat aggaggctgc
    16621 ttccacatcc aataaggcat ttttgtcgac ttggcctgct gcgtacaatt gacgaattct
    16681 tgctttttcc tgattggaga taccagaggg cattgggccc gcgggtacaa aaataaaagg
    16741 cagatggcca aatgccagcg cagccattaa taatccaggg actattttat cgcaaatacc
    16801 taagaggagc cctccatcaa acatgttatg tgacaaagca acagcagtcg ataaagcgat
    16861 cacatcccgg ctgagtaaac ttaattccat ccctggttgt ccctgagtaa tcccatcgca
    16921 catggctggg acgcccccgg caaattgcgc aactcctcct gcagcagaga tcgcttcctt
    16981 aatcaaatca ggaaaaacag catacggttg atgcgccgat aacatatcgt tatatgccga
    17041 aataatggca atattggctt ttgcattacc tctcaaaata gccttgtcct gtttagcaca
    17101 agcagcgaag ccatgtgcca aattcccaca atgcagaata cttcgttgtg ggcctttagt
    17161 acgagcctta tcgatttgat tcagataagc tgctcttcta gaagcgctac gattttggat
    17221 attttctgtc acttgattta ttacaggatg cattcttttt cctcatcgct tggagcatac
    17281 atcacctgaa cattggcatc cggggcattt aaaatagcgc agattggcag gatctccggg
    17341 ttgttagttg ccattgcctg ctccagaaca acccgttttc tttgacctac taaatgtaaa
    17401 aaaatagtac tgctgtttaa taatcgcctt ttcgataggc taaccctctg gaatgaagct
    17461 gttctaggct tcacaataag caccgcattt gctttatcat ctaatcctgc acttaactca
    17521 tcactgcatg gaaataaaga agcagtatgg ccgtcttcac ccattcctaa aactactgca
    17581 tcaaatgttg gtagagagtc aatttggcta ctcacctcat tcaaatgacc taatggctcc
    17641 tctttttcat caaacaagct gataaatttg gctttttttg ccttctcttg cagcagaaat
    17701 tcacgcacca gcagctcgtt acgatgtgga tcaaaaaggt tgacacaacg ttcatcggcc
    17761 aaagtgatca ctactttttc ccaaggcaag tcggctaaag ccaattcctt gaacaaatcg
    17821 ataggggttc taccgccgga aaccaccaaa aaagcttgcc ctcttcgttt gattgcatct
    17881 gatagaatag ttcttaattt ttccaccatg tcatgaatca actgtttggc atccctaaaa
    17941 ctatgaaact gcattgtgat caccccaaac atgtgcttgc ccgttaaata agcgtatgac
    18001 ttcctttggc ccccaggtac aagctggata ggtatacagc ggaatattat cttcttccca
    18061 tgccgataca atactgtcaa tccattgcca agcctgttca atttcttctc ggcttacaaa
    18121 aagatactga ttacctaaca tgacctctag caataatctc tcataggcat cagcaatcct
    18181 ttgcgtttta aaactatgat ggaaatttaa atccaacttg gaatcatgca acatcatacg
    18241 ctcaccaaga cccggaattt tattcattat cctcacctcc accccttcat cgggttgtaa
    18301 gcgaataatt aactgattag ggtacaattc ttcatttaag ggatgaaaga tattataagg
    18361 ctgtggttta aaataaataa ccacttcgct ttgctttttg ggtaatcttt tgccagtaag
    18421 catatagaat ggaacaccag accatcgcca attatcaaca taggctttga tagcaacaaa
    18481 agtttctgtt gtagatgcct gattagcacc cacctcttcc aaataaccag gtattttttt
    18541 accatttgct aaattattag ccacatattg agctcgtact gtatgatcat gagcattgga
    18601 cttgttaata aaacgcaaag atttcagtac ttttaatttt tcgcttctga tactttctgc
    18661 tgaaaggctt aaggggggtt ccatcgcaac taaagataat atttgtaaca aatgattttg
    18721 cagcatgtcc ctgacttggc ccgacttatc ataatattcc caacggcctt caacaccgat
    18781 ttcttcggca acagaaattt caactcgatc aataacgcga ttatcccaat tggttgaaaa
    18841 tatggaatta gcaaagcgaa gcactaataa attcaagacg gtttctttac ccagataatg
    18901 atcaattcga taaatctgat cttcagaaaa aaaacaagcc acttgttcgt tggttgctac
    18961 agaagaaggc aaatcatgac caataggttt ttccaaaaca actcgcgatg gtttctgggt
    19021 tagattaagg cgagcaagac tgcggcaagt ttgagaataa aggcttggag gaattgccaa
    19081 gtaagaaata accactcgct cagaagggtt aacatagtcc gttaattttt gataatcatc
    19141 tgattgattc agatcaagct tgcaataagc cagtttcttt ataaaacgta accagactgc
    19201 ttcatcaatt tcatcaaata aaaattgctg aattttattt ttaatcaagt cagcataagc
    19261 agaaaaatcg agctcttctc gggcacaccc aataattctt gttttttcgt gaagtaaatt
    19321 agccagttct agttgatata atgcgggcaa caattttcga caagacaagt cacccagcgt
    19381 accaaacaaa attaaatcac aaggatactt cgattctgct tttaagagca tatatcatcc
    19441 caaagttaca ataactatgt ccctgaaatt gcatcgattt tacatcactg gaatcaagcg
    19501 tcaatcaaaa ttgatttttt tcgctcattc tatcaggtac attccctgtt taatcattca
    19561 aatcaattga agtgaattaa aagcggtaaa ggataaaaaa ggattaaaaa atccacttgg
    19621 aaatccgctt tatgaattgc aacattatca aaattagcga tttttgtcta aaaattgcaa
    19681 aacttcattt ttatgctata tgaaataagt acacgttagt aaaaaatccc tttgatcagg
    19741 agccactcat gcttaacaag agattttcag aacgactgaa taaagaattg gataatattg
    19801 gagttcctga ggccacagca gagcgaattg agatactttc caaactcatc aaaattccaa
    19861 aatttaaagc agaagctttg cttaatggaa ttacgatccc tgatgaagcc gtattaaaaa
    19921 cattagctca ggaactggaa gtcaatgtgg attggctaat tggcaaaagt gactccaaat
    19981 caggagccca ttagaaatta tccatgaaat ctatgattgg aactaattgg cttcgctttt
    20041 ttctcaatgt attcaatgat ttttcctgcc aaattaatat tagttgcttt ttctatacct
    20101 tcaagacctg gagaagagtt aatttctagc actaatggac catgatttga gcgaatcaga
    20161 tccactccgg caactcttaa tcccattgtt tttgcggcac taacagcgat tgcccgttct
    20221 tgtggggaga gtttgacttt tactgctttt cctccttgat ggacatttgc gcgaaactcc
    20281 ccatccttgg cctgccgttt aactgcggca actacttttt ctcctatgac aaaacaacgt
    20341 atatccgtac ctcgggattc ttcaatgaat tcctggacca aaatattggc atgcatttct
    20401 ttaaaagcat tgatgatact cacagctgat tgatgactgt cagcaagaat gactcctttg
    20461 ccttgtgttc cttctaataa ttttatgacc agaggagccc caccaaccat atgaatcaaa
    20521 tcttcagtat catcaggaga ctgggcaaaa ctggttaacg gcataggaat tcctttacgt
    20581 gccagcaatt gcaaagagcg aaatttatcc ctggatcgtg atatagctat ggattcatta
    20641 agagtgtaca tgcccattgt ttccatatga cgcaatactg ccgtaccata ataggtaatg
    20701 gatgcgccaa ttctaggaat aacggcatca aaatgcggta aaggcgcacc accccgataa
    20761 tgtaccttag gattagaagc agccacgttc atataacaat ataaagggtt aataatttta
    20821 acctcatgcc ccgcagcctc agcctccgcc tttaaacgct tatgagaata caaatggggg
    20881 ttagttgcca atatcgcaat tttcatgaat taaactttcc tcagtaattt catacttcaa
    20941 tctgaacaat ccaaaccatt ttccctaatt ttctattaaa taacgcaagt tttaagcatc
    21001 acagcagagc tgctctgaaa tgacaaaagc agccaagcgt agaaaatcgc gggttttaag
    21061 ccaagataaa tgaaatgtca acaatcctta cttaaagcct agattaaaaa tcacaaatta
    21121 tcctccctgc cgtacccaac aggagagtaa tttttgaatt catgcaacag tcaattaatt
    21181 aactaatgca ttctagatag atttctttga cataaataaa acaagtctgc ttagccattg
    21241 ctcctgtaga ggtaaaatat cgaattacag gttaagatac caataagaca taatccttag
    21301 ctaagagatc cgcatgatgt ggtgttgtaa aaaatgcatt taactcacct tgaggattaa
    21361 atagcattac ggccccacta tgttgtatat cataattttc aggctccgaa ctctctttat
    21421 tgtttatttt tgcgtaagca atgcccattt catgggtcat tgatttaatt gaatcctctt
    21481 ctcctcttgc accataaaat gatttatgga aagaagtgac ataactaccc aatttatcaa
    21541 gactgtctct ttgcggatca atagatatca tcacaacttg aggtaaattt ttcacccctt
    21601 tcttttccag aattcgatac atttttccaa gctctgccat ggtagtcggg catagataac
    21661 cacaattggt aaatccaaaa aatactaaag tccagcgccc ctgcaagctt gagttattga
    21721 aagccttctg atctgtgcct gttaagctaa aattattgac tgttcttgga ttttcaagat
    21781 aagtcccatg aaaatccgcc acattgatct tctttttaaa atgaaaattc tgagagatat
    21841 aaataccagc taataaactg accagggcca atacagtgat tgttacagcg ccccctttta
    21901 tctttatgtt cattctaacc ccttaaaaat agtgatctac taataaaaag acaaacaaca
    21961 gcattaggta cagaatagaa aatcggaatg tttgcatagc aacgactggt ttatcagtaa
    22021 aatacaattt atgtgcccaa accaaaaatc tgatgcccaa aaccaaagca cctagcaaat
    22081 aaatccagcc actcatactt acaacaaaag gcaaaagact gactaccagc aataaaaccg
    22141 tatagagata aatatttaat ttagtgaatt gaataccgtg tgtcacaggt aacataggaa
    22201 tttcagcatc ttgatattct ttatatcgat aaattgccaa agcccaaaaa tgaggaggtg
    22261 tccatgtaaa tataatcaat actagtaata aggcctgagg atccaattgg tcagtaaccg
    22321 cagtccatcc taataagggg ggcgcagctc cggccaggcc cccgataaca atattctgcg
    22381 aagtggctcg ttttaaatac cctgtgtaaa caccagcata accaatcagt gttacaaagg
    22441 taagtaatgc tgttaattgg ttcacaaaca ggataagaag acttaatccc attagcccga
    22501 taatgaccgc gaaccataag gcttgcttta ctgacacacg tccataagcg actggtctct
    22561 tttttgttct ggccataatc gaatcaatat gcctgtctac caaatgatta attgcagcag
    22621 cgctgccagc gcacagtcct ataccaagta aagtaaaagc gattaaccgc aagctcaccc
    22681 agccaggagc tgctaaatac attcctacaa tgacagtgag tagcatgagc aacacgacgc
    22741 gtggcttaca caattccacg taatcacgcc aatccacagg aagtcgagct gcgtattcag
    22801 tacgcatggc taacctccct atcttgtgta aaatgcaaag aacaaaatgc caccgctaat
    22861 aataatgcgg ctacaccatt atgagcgacc gcaaccttga gcggtaatag atgaatcaca
    22921 ttcattatcc ctaaagtgaa ttgaagtaat actaacatag ccatgataac agcagttgct
    22981 cttaaataaa ttgaatgcgt tttgaatacg attattaatg acagagccag tagataagtg
    23041 gcagtcaata tagcccctat tctatgaata aattgaatgc tggcccgaac ttcactctcg
    23101 agaacaccgc cttggtaatt ggcgcctatc gtagaaaata aattaaatcc ttttgcaaaa
    23161 tgcaattcag gaaaccaggt tccgttacat aatggaaaac caatacacga aatacctgca
    23221 taattggcac ttacccaacc acccaaagca atttgcaaca aaatgataac aattcccaaa
    23281 cgcaaccacg gtcgccattc aggtaaattt tgacccttta gctgggctat ctgcaaacga
    23341 aaacggcata aacaggcaac gattaacatg cctcccagca aatgtcccat gacaactaca
    23401 ggcagtaatt ttaaagtaac agtccacatt cccaatgccg cctgaaatac cacaagcata
    23461 aataaagcca agggaagatg ccaaggcaaa tcttgatcct gacgacgttt gaaccatacc
    23521 tggaaagcaa tgaaaaaaat caacagggct aacgttcctg ccgcatagcg atgagccatt
    23581 tcggtccagg ctttcttgga ttcaatagga aggtccggat attgtgattg agcttcttgc
    23641 aaacctttat ctgctcctgg aagaaccatg cgaccataac aacctggcca atccgggcag
    23701 cctaagcccg catcagttaa acgagtataa gcgcctaaca taacgacaaa aaaagccaaa
    23761 aaaacagcaa gagttgctgc taatcgtatt gaactattag acatcaattt atccgctctt
    23821 aatttcagtt gtattgagta aaagttttaa atctttatat atatcacctg ggttacaccc
    23881 tggttgatag ctcagtatga ggtaattatc cgggtttgct ataaaaattt tagcttcagg
    23941 atataaagtc gacaatttag acatttcagt taatgataag gcagcaacat ggatatcttg
    24001 ttcttttaaa atgggtttta attcctctgc gatcaatgaa tctttgttgc ttagaagcaa
    24061 ccattcatct acctgatata attttctccc aaaagaaaga cgaactcttc ccaacatatt
    24121 aagctgctta agacaagtct tgtcacaacc attaggattc caatagataa tgcgccattt
    24181 tgcactgcta tctaatggtt ttagagcgat aggtggagaa agtaaaattc ctttatttac
    24241 tttagaggtt ccaagccagg aaggattttt atagaacaaa taagcggtta ttcctggtgc
    24301 agcaaaaatc actaccaaga gtagtaatgt catgtattta tatggttttt tcatttttct
    24361 tcaaattaaa aatcacgaat ataatcagaa taaccaatgc catggcaaac cactgtattg
    24421 cataagcaaa gtgcctttgc gggggcatgg atactatctc ccattcacgt acaaaaccaa
    24481 aaggctcatt cttgtcgagg cgaataataa acggatagac ctttttttgc aaaagttgtt
    24541 tcagtatttc ttcatcaatg agctcaagaa gagtcacctt attctctttt tcttcaaaac
    24601 ttggccctaa tatccattgt tttttacttg gaaaataaac catgccaaat aatttaaatt
    24661 tgccgttggg agtctgaaca ttaggaaaag tgcgtcgagt aatgtctcca gaaacccatc
    24721 ctctatccac cataacgatg gagccatcat caagaagcat aggagaaacc acgtcataac
    24781 caaactgatg ttgataatgt tgattatcaa gcaaaaataa ctttggcaga aaagctcctt
    24841 caatgctgat tcgttgatat tgctctggca atttttgccc tggctgccaa attatgggct
    24901 ccttctttgc cagcgcttgt tgtgcggtta tcatttcagt tttttcatcg gctctgtgta
    24961 tttgccaaaa tccaaggcta atgaataaaa aaaagcaaac ggccgtaaga ataagcattg
    25021 gccaatttga ggtgaaacga aaattaaaac aggttaaact aggcatcttt tatcaactac
    25081 tcatttggtt agctaacatt cccctgaagc tagcaatgtc aggagtataa caaatgttca
    25141 ctaaaatctt catcatcttt gtcatgattc ttattgtggc ctcattagcg agcgggctct
    25201 tttacctggt caaagacact gataaatcaa agcgcacagt caaagcgctc accgtgcgca
    25261 ttgcaatttc attatcttta tttgtgtttt tatttattgc cttcaaattg ggattgattc
    25321 agccacatgg aatttagctt ttggctttta ataaattcac ctcaagagat agggtcatgg
    25381 ctgagataat gagatatttt taaatagtca tttcagccac cttcagaaaa ttccccctaa
    25441 tccacgaaac gacaaacagc gagaaaaaat ctcccagcga gcatcaattg accgtaaacc
    25501 aataccaccg ttccaggcag gaggggctac tctcttacaa taaaatttcg aaattgctca
    25561 atattctatc tatacttttg ttgtaaccct caaaagggat ggtgagcatc atgatgatag
    25621 ttctgttttt attttatttc ataataggat tacaaattat atttaaaccc aataaaacga
    25681 taatactcca atttcttttc agtttgtttc ttatactaat cagcttcaat tatcacagcc
    25741 atctggtgaa cctatgaata aacaaatgca atggcatctt ttcgggaata cttcgggctt
    25801 attgataatt tgttgccttt tgatcttggc cttcctggat caattcatct tttatgaact
    25861 accctgccct atatgcattt tgcaaaggat ttgctttgtt gcagtaggtc tctgttttgt
    25921 tatgaattta atgctgggta ttagagcttc acattacggg ctgatgcttt tggcaagtct
    25981 tttagggctg tctatctcgg ttcggcaaat gtatattcat ttaacacctg gtgatcctgg
    26041 ttatggcggc ctctttttgg gtttacatct ttacacttgg tcagcaatca tatttttagt
    26101 cattatctta ttaattgctg gggctctaat ccttgataag ggcttcatgc cagattataa
    26161 agtacgaagc aagtcagcaa aagtaatgat atgggtattt tttatcttga ttcttgccaa
    26221 tggaatcagt acattcttgg aatgtggacc gcacgaatgt ccagataacc cgagcaaata
    26281 ttatttacta aataataatt aaggatcttg aggatttaaa atgcgtcttt tgtttatcag
    26341 tttaattctt gccagcggat atttacaggc cgatacaata aataattata tgaatattgc
    26401 taacaatatt ccgcaaatgg aaatgaaagc agaccctcaa gctcaagcat gggccagatc
    26461 ggcacgtcat gtacttacca ttaccagtga aagtattgct gaaactttaa tccaggcgaa
    26521 tgaagccgca aaaacacaag gtaaacctat cttttgctta ccacaaggtg tgcaacttaa
    26581 ttcttttgta atgaacgagc tcatacaaca aacctataaa gaaatatcca gtcaacaaag
    26641 cgacaaagat aaaatgaccg tttctcaagt ggctttatta gggcttagca aaaaataccc
    26701 ttgtgaaccc agtcctcagg agaaacaaat tcaacatgtg gcttccctgt tatcgccaca
    26761 atagagcccg cagcaacaaa gtcccggaat ttttttctac atccgctgct ttaagccaga
    26821 cgacatgttc tgaaaattgt tagagtaggc atggcaaagc aaagttggca gagcgccaaa
    26881 cactgtgagt tcccttgcca atgtttgcaa aaactctgcc ttgctggttt tttattgatt
    26941 ttagtgttta ttaagcgtaa ctttagcttc tttataatta cttactaaat tcctcaaaag
    27001 cttccaatgc ttctgcagca tacattagag aagggccgcc tcccatataa accgccatac
    27061 ccaatgtttc cagtaactct tctcttgtcg cttgcagttt cactaaagtt tgtgaatgaa
    27121 accctatgca tccggggcat tgcttggcca cggccaacgc catagcgatt aattccttgg
    27181 tttttttatc cagtgcaccg tctttcgtcg cagcttgtgc caaagaagaa aatccagtca
    27241 ttagctctgg catctctttg cgaaacttgg ctaattgcgt agtgatatct tttgtaatgt
    27301 gtgaaaattt gtctgacatg aggctctact cctttcctta ttatattttt tcaagcgtct
    27361 ctaatttaca gcataatatt gggatgcaaa agatgtattc cctaatttaa cgtgagtaat
    27421 gtataagata ggtcgttatc ttgaatcgtc caggctgagg atatcgcctg attcttatac
    27481 aatccctatc gataactcac gttaatttaa gaagtggcga tttcaggata ttgctcgatt
    27541 gagcgtttcc ttagccagag taagtctttt ttcaacattc cctaattcgg tttgcaaatg
    27601 caaaatagtt gcaggattag agcaggactt catttgctct aaagtttttc tcatggcttg
    27661 atgttgctct tccaaggcac agatattctc ctccatggtt ttaatccatt gttgtcttgt
    27721 actaagagtt gtcaaatcaa aagaaatttt tgattcaggt ttattattct ttttgtccat
    27781 ttggcataaa atgaccaaga cattaatttt tttctggata cgctctgcca aaaagcatgc
    27841 aattgactca tccttatgtt tctgtaaagc gtgaatatca tccctgatct ccttgataca
    27901 ggcaggtcca ttgaattcta taacggaaga aaatatgcct ttgggcaaat tatgaattga
    27961 aatagaagaa ctcaatccat tgatcttcca ttccagctct ggcaatcgtg cagataattc
    28021 ttccaacaat tcgtcaatat agcccatctt ttgaaaatta cataaacaac tggggttatt
    28081 aaaattaacc tgtcttgcta aattatacag caggttccaa atccgcctct ttggcttgca
    28141 accaactggt tataaaagga ggggcacaca tgagcagcaa accaataatc aaggtcaatt
    28201 cataacgtgt tgcactgccg acattaatcc cctctggagg aataaagcta actgctaaag
    28261 tggtaaccac tccaatgatg cctatgcctg caacaaacaa cattcctgcc ataccacctg
    28321 gaattttaaa aggtctggga tggtaaggtt ggcttatgcg caatttaatt gctgcgacaa
    28381 acatgatcaa atacatcagc atgtataatt gagccgccaa tgccgttaag agccagtagg
    28441 atccattgac actgggcata aataagaata aagccgataa tactgtgact atggttgcct
    28501 gggtgtatag catcatggcc ggagcacctt ttccattagt ccgttgaaat acctccggta
    28561 aattcccttc ttctgcagca actaataaac ctttggtagg ggcaatgatc caattactca
    28621 caccaccaag ccctcccaga accaacatca cagcgaccac aggcatcatc caatccatgt
    28681 gatatttaag gaaaaaggca gaaaaggctt gcataattcc ggcaaccaga ttgatatcct
    28741 tttgtgggag tactaccgca atcgctaatg aacctaaaat caaagtactc agtatgatcc
    28801 ctacagaata aaccagggct ttggggaaag catgctgggg atcctttaca tcattagcat
    28861 gcactgtagc aatctctatc ccgcaaaatg acaatatgat ggcggttaat gaaacccaca
    28921 tgcccttatc cgataaatgg ggaacaatac tgggcatgtc aaagctgatt tgcaaggggt
    28981 taccttgagc tatccaaaca gcacccaaac caatgatcaa agacatgggt aataataatc
    29041 ctgccaaaga acaaatatta ctgaacatgg ccgatgattt catgccgcgc aaattcacaa
    29101 tggttgctcc ccaaaagcaa ctgacaatga ctgcccacag aaaataagga ttggtagtta
    29161 aagagggatt aataagataa cctattgttc ccgcaacaaa cgatagaatc gtgggatacc
    29221 aaatcacatt ttctatccat tgcaaccaaa tggctaaaaa ccctgatttt ttcccaaaag
    29281 cttccttaac ccagatataa acgccaccct gcctggccca cccagaagct aactctgccg
    29341 agaccaatgc ggttgggata aggaaaaata aggcacccag gataaaataa aaaatcaatt
    29401 gactcccaaa taatgcagta gctggcaaat tacggatact gtccacagag ccaacggtaa
    29461 tcatagtcaa gctaaatata gtgagagaat gttttttatt gctcattgta ttccttcagt
    29521 gaggactatt tggaatcaaa agtttattat aaagaaataa gcttaagg
//
LOCUS       DATNSN010000024        29340 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.024, whole genome shotgun sequence.
ACCESSION   DATNSN010000024 DATNSN010000000
VERSION     DATNSN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29340
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.024"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..1306
                     /gene="nhaA"
                     /locus_tag="VAH76_000865"
     CDS             158..1306
                     /gene="nhaA"
                     /locus_tag="VAH76_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEO1385512.1"
                     /translation="MTKSDSFYNLETLGGILLFIAAVIAIVVANSPYRHIYDQLLHIK
                     ATVGVGSFLIKKPLLLWINDGLMAIYFMLIGLEIKREIRRGVLSHKKNLIVPIITALS
                     GLLVPALIFSWFNVHNPVYLQGWAIPTATDIAFTLGIVSLLGSRVPLSLKILLTAIAI
                     FDDIAAIVIIAFFYTKNLSLLALSFALLFTFILIGLNYFKCRRVSLFIVVGIFLWIAV
                     LKSGVHATLAGIVIAMTIPDESRGSMLARLEDGLHPWIVFLILPVFAFANAGVTFLGL
                     DASMFIHPIVLGIGLGLFIGKQIGIFLSLGYFVKFKHLLKADNITLGQVYGIALICGV
                     GFTMSLFIGSLAYQHNDLNLMPLVKIGVVLGSLTSGILGFIVLKRASS"
     gene            1418..2749
                     /locus_tag="VAH76_000866"
     CDS             1418..2749
                     /locus_tag="VAH76_000866"
                     /inference="COORDINATES: protein motif:HMM:NF021524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEO1385513.1"
                     /translation="MKSRLLNFLSETRASYWYIPLLMSIAALLFSILSLRLDHLFVLH
                     WLDTWGWFHASNPEGARAILSTIATSMITVAGVTFSMTIVAVAFAASQVGPRLISNFM
                     RDRANQITLGTFIATFLFCLFILMALYNANKVGVSESDGLVFIPQISLLMAIFFTISS
                     IIVLIYFIHHIPESINMSNVIAQIGEELNRQVDCLFPINVGKEMSKQSAHIPKNHQHR
                     DAVVATKLGYVRILDGNTLMAIAKKHDLIMQLEAGYGTYVTEGSTLLYIYSSEKIEKL
                     IHKQCVDAFALGHQRNQEHDIIFLIDEMVEIIARALSPGINDPFTAMTCLDWLQSTLQ
                     KISKTSQPSAYRYDTEDKLRLIAQPIAFTEFCDLIFCRVQPYVCKDRNTAIHMMEMIN
                     TIHARITNDEHKITLTSHAASLKNAALECLSINEDRKKLQSLYEKHFVNSL"
     gene            2986..3186
                     /locus_tag="VAH76_000867"
     CDS             2986..3186
                     /locus_tag="VAH76_000867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385514.1"
                     /translation="MTKTLKYLMHELKGKTLLVSVGDEKFDVDLNDFDSYSHEGMTFI
                     KVTASGKTRYINQAKIKYFEIT"
     gene            3284..4432
                     /locus_tag="VAH76_000868"
     CDS             3284..4432
                     /locus_tag="VAH76_000868"
                     /inference="COORDINATES: protein motif:HMM:NF037527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385515.1"
                     /translation="MDLLTQDFPDTFLNPSELPCISLYQPTHRSHPDNEQDVIRFKNL
                     TKEIQHSLEKKYSKSTIQSLLKPFHSLAEDFSFWTHNRDGLAILANPQLFKVYKLSHP
                     VPELTVVADSFHIKPLIRIMQSADRYQILALNRQQIKLFEGNRNGLEKIELENDFPST
                     ITKALGKELTQPYVTAGSYGMGTEGPSMHHGQGSKKDEIDVDVERFFRVVDEAILKFH
                     SETSKLPLLLAALPEYHNLFHKISKNPFLIKNGIQINTDDLSIEELCKQAWKHMEPHY
                     LERLDTLNKQFYEAQSNELGTDILKDIAKAAVSGRISTLLVDADRQIAGQIDHESGDI
                     KLDELVNPDIDDLLDDLGELVLKTKGEVIILPSERMPTNTGAAAIFRY"
     gene            complement(4511..4840)
                     /locus_tag="VAH76_000869"
     CDS             complement(4511..4840)
                     /locus_tag="VAH76_000869"
                     /inference="COORDINATES: protein motif:HMM:NF020469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEO1385516.1"
                     /translation="MLVTFRCDVYPDITMLDDVAIPLLKIMGHSGSVPGAIIAKDVPA
                     ALTRLKSAFKQENNSAELTISNPQSEEEDEDVSLETHAFPLIELFSCAAKEKCNILWD
                     KGTKVPF"
     gene            complement(5116..5565)
                     /locus_tag="VAH76_000870"
     CDS             complement(5116..5565)
                     /locus_tag="VAH76_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HEO1385517.1"
                     /translation="MSVKINEAIQDIAVKHGVVLGKDDPVLILQTMNERLIEENRKAQ
                     QDMLTHFKEEMEDISSQWKDDAKEKAEKVLNAALASSKEAMDKILRETTSESVHAMKR
                     LISESLTEARYLTQQTRKTNWFTLLSSVAMLTVPCAFMLLFLIDFLR"
     gene            complement(5565..6290)
                     /locus_tag="VAH76_000871"
     CDS             complement(5565..6290)
                     /locus_tag="VAH76_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HEO1385518.1"
                     /translation="MAKIHITMQGKGGVGKSFVSATTAQYKLYKEQPPLCFDTDPINA
                     TFHGFKSLNVERLDIMDGDEINPRHFDALIEKIANTQNDVIIDNGASSFVPLSHYLLT
                     NQVPTLLKGLGHELIIHTVITGGQALLDTTHGFSQLVNQFPQDVRFVVWLNPYWGKIE
                     SEGRSFEQMRVYKETKDRIAAIIKIPDLKEETFGLDLSNMLQQKITFNEAIDSPDRNI
                     MTRQRLKLIRDQLFSQIDNAMVI"
     gene            complement(6290..6616)
                     /locus_tag="VAH76_000872"
     CDS             complement(6290..6616)
                     /locus_tag="VAH76_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HEO1385519.1"
                     /translation="MKKPLSERVIQSQSEKKARRISAKIEFIALQEDIKEALDKGCSM
                     KAVWETLSDEGHISFGYKAFRHYVLKLIKSAQENTKDEKQGKSKTTHEIKGFTFNPIP
                     NPKELL"
     gene            7021..7374
                     /gene="traJ"
                     /locus_tag="VAH76_000873"
     CDS             7021..7374
                     /gene="traJ"
                     /locus_tag="VAH76_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HEO1385520.1"
                     /translation="MDEKLKTPTRKNGRHLRVPVLPSEEIQIKSNAATAGLSIAEYLR
                     RISLGYQIQSSIDKDHILQLAKINGDLGRLGGLLKLWLTQDKRVAHFDLQTVKTLLNR
                     IQTTQDAMLEVVKKL"
     gene            7371..9239
                     /locus_tag="VAH76_000874"
     CDS             7371..9239
                     /locus_tag="VAH76_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO1385521.1"
                     /translation="MIIRHIPMKKTRLSSFSGLVKYLCNQQNKQERVGKVRLSNCNSL
                     DPIWAVQEVLATQAKNQRATGDKTYHMLISFAPGENPSPQVLREIEDRVALSIRLKEH
                     QRISVVHHDTDNLHIHVAINKIHPQTFNMIEPYRAYKTFAEVASQLEIELGLQITNHQ
                     TRKNRSENLADDMERHSGIESLINWMKRHCKEKIEAVRSWKEIHSILAEHGLMIRVKA
                     NGFVFCNVQGLTVKASSISRSFSKKNLESKLGSFIASYSEGDAPESNVYRYEPLNKKV
                     LGSELYATYKHEKSHNKHIISDKLKNLREARAKLIEKAKKRGRIKRAALKLMNLSKIQ
                     EKFLYSHINKTLLNEIDSIRKNYAKERSKLLDSHRNKIWADWLKQKAQEGDQEALVAM
                     RYRNRKNNHDYTISGKDSVSTIFNFRKIDSVTKEGTEIYKKDKSVIRDNGKEIFISKG
                     GSISALKRALDMARQRYGDCICVNGSPLFKKIILQIVIQYQMPITFADLDLENQRQKL
                     NFEQEKSHEQSRSSGQNHRGRTSGGYETIGAANENRKSRGTKPNTFSIRQGSPAKDHN
                     GLRNLSQCDLVQFTRRGQVLLQDDAHDHLERQGFKPDNHVRREVSRLKKNRKGYKN"
     gene            9406..10089
                     /locus_tag="VAH76_000875"
     CDS             9406..10089
                     /locus_tag="VAH76_000875"
                     /inference="COORDINATES: protein motif:HMM:NF024949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1385522.1"
                     /translation="MTNEQNNGTYGPIPAKYLAFYIVLYRKQRGWTQETVAELTKLNV
                     RTIQRVENGKGSSPDVRRALASVFGLEDIDIFNRPFQHPDEVALREEYERLEKETITL
                     SVKKVTCGKQLREMAEEAQAHQFEAREGLSKESEHCFAELQDYLQDCDGIYQEMTAVQ
                     KLEINEELQSMLERFKNAGVSLGIAVGSLKMGDDKDPFSIRSNCYIAAPNDSFPEKIM
                     LNKSVRMSM"
     gene            complement(10206..12392)
                     /locus_tag="VAH76_000876"
     CDS             complement(10206..12392)
                     /locus_tag="VAH76_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HEO1385523.1"
                     /translation="MTKMPYHEVVANQIIESLKAGTAPWLKPWEPGVGNGQIPYNPVT
                     GKRYRGINALYLMVNQSDDNRWLTYKQAQSMDAQVRKGEKGTTIQYWKFHEEQTKHDD
                     TGKPVLDEQGNPLKVQVNLERPKVFYATVFHASQIDNMPELITKEPDWSLMEKAEKLL
                     HNSGAKIVHSEADKAFYRLSTDSIHLPPKEQFKSAANYYTTALHELGHWSGHPSRLDR
                     DLGHPFGSDAYAKEELRAEIASMLLGAELGIGHDPSQHTAYIKSWIRVLEDDPLEIFR
                     ASADAEKIVNHLCALEQTQNINLGELVEIKDDKLKEEVVMASENIRSEKVWLSIPFQQ
                     KEIAKSTIGKLPDGTPGIAWDKVQKCWYANPGVSVEKIKAWLPEHQVLTQEKALIPED
                     EFKAALISLGAIVSGEHPIMDGKPHRISTEGDKNSEKAGFYVAHLDGIPAGYIKNNRT
                     GTELNWKCKGYILTDEQKSTLKAEALEHQQIRQRELESKQKSTALKLKQKLSLMTEVV
                     EPTPYMQAKGILNHYGVYTDSEKRLTCIPATDCEGTLWTIQYISEDGAKRFAKDSKKE
                     GCFHVLGGLEKLAETPVIVITEGYATAATIKDATKLPAIVSAFDSGNLKPVAKALRDK
                     FPNTPIIVASDDDKHLELTKGINPGKEKGSEAANSVNGFMILPTFAPGEQLSNPRQFS
                     DFNDLANKSQLGIAGVKRQLKSKIDSVAKQNSRLQLTQKSNVVRIT"
     gene            complement(12385..12549)
                     /locus_tag="VAH76_000877"
     CDS             complement(12385..12549)
                     /locus_tag="VAH76_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385524.1"
                     /translation="MNHETELMDVIAEKFEDLVIPGFLLEVSPIEADIMGAFVEDALN
                     EEDAMEAIYD"
     gene            complement(12539..13090)
                     /gene="traF"
                     /locus_tag="VAH76_000878"
     CDS             complement(12539..13090)
                     /gene="traF"
                     /locus_tag="VAH76_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HEO1385525.1"
                     /translation="MKKLSIFTSMFLISLIVLCVLFHTMGFRINLTESIPIGLYRITG
                     EAPIKNAYVIFCPDSRKSFRLAKDRGYIDHGLFCDGYGYLMKKVVAVSGDILSVTNKG
                     VFVNQILTPYSKPKPRDGMKRSLPQWQVMNYQLQNDEVMTMTSQSEWSFDGRYYGLVH
                     TGQLKGMITPIWVINKRREPHES"
     gene            complement(13087..14976)
                     /locus_tag="VAH76_000879"
     CDS             complement(13087..14976)
                     /locus_tag="VAH76_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO1385526.1"
                     /translation="MSQNKPNKAIGPQVREKNHSKINRTLVLLVICSFLISMHIGTQF
                     FAHQFHYADALGGTFAPIYMPWQIFTWAIKYQPYYPDSFNAAFGLIVMMGCLFLMMIV
                     LASKYFKKNSVSDYLHGSARWANWEDLNSASLIGHDEGVYVGAFEDDKGAIHYLRHNG
                     PEHVLTYAPTRSGKGVGLVIPTLLSWKHSCVITDLKGELWAMTAGWRQKHATNKVIRF
                     EPATLKGSARWNPLDEIRVDTESEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGF
                     ILHAIYKLKTQGEPATFPNIDRMLVDPNINIADLLIEMTQYPHVEGKTHPVISASARD
                     MIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIRDLMNHECPVSLYIVTQPN
                     DKARLQPLVRVMINMIVRLLADRMEFERVSDARGLSYVSAKKTYKHRLLCMIDEFPSL
                     GKLDILQESLAFVAGYGLKFYLICQDINQLKSRERGYGPDETITSNCHIQNAYPPNRV
                     ETAEHLSKLTGQTTIVKEHITTSGKRFSTFLNQISKTVQEISRPLLTVDECLRMPGPK
                     KDANGLIVEAGDMVVYAAGFPAIYGKQPLYFKDPIFMTRASVDAPAHSDILRTRLSED
                     EVIRL"
     gene            complement(14973..16355)
                     /gene="trbL"
                     /locus_tag="VAH76_000880"
     CDS             complement(14973..16355)
                     /gene="trbL"
                     /locus_tag="VAH76_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEO1385527.1"
                     /translation="MNKKIIPYVIAFGLFGFSACCHAKGHGMDSRDLLDNILQRFAST
                     SALWSKTMLSYARYLFWSLASISMVWTYGLMALRKADIQEFLAETVRFFVVVGFFYWI
                     LDNGPAIAKAIIDSMRQLAAKASGINEHVSPSDIVDVGFDIVSKAIDSSSIWSPAATT
                     VGLLVAGVILIILALVSINMLIILITAWILTYGGIVLLGFGGGRWTQDIAINYYKTVL
                     GIALQAFAMILIIGIGKSFVDQYYAAMSENIMLKEMFVMLVVAVLLLVLIDKIPPALA
                     SIVSGGGSGGGGGLGLGGAMGAAGIAGAAMAGAAGSVLAQSGGGLSALNAAFKAASQS
                     TNSGDTNALSGMDKSSKTGGLAEAMGSAAKFAGSFGSHLASGTMDIAREKANSIKEAV
                     AEKIADTTGGKIAEAIHNKVDTSTSESQESNAQNILSMGTPAGSLSSGFETSDEVSQF
                     VNQKASGESQ"
     gene            complement(16360..16572)
                     /locus_tag="VAH76_000881"
     CDS             complement(16360..16572)
                     /locus_tag="VAH76_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385528.1"
                     /translation="MKILFLSIMVVVCVLSGCDSAESKAQKKAEHLATLTCSSPREGR
                     TTEELQAIGDACFRGGSYSKSSGKKW"
     gene            complement(16596..17336)
                     /gene="trbJ"
                     /locus_tag="VAH76_000882"
     CDS             complement(16596..17336)
                     /gene="trbJ"
                     /locus_tag="VAH76_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HEO1385529.1"
                     /translation="MKFKSMIIYCFVLPVFAGGAPVFDIANWIQNGKMIATQLSEYKT
                     QVDQYKNQMDQYQNMLDNTKSLTSFEWDNANSVINNLLESTDTIDYYKQEAGSLQGYL
                     DRFQSQEYYQKTPCFNGTGQCSAEELRKIKQSRLAASVAEKRSNDAMLKGIDKQQQSL
                     KNDSAKLRTLQSQAQSAAGQKQALQAASQLASNQSHQLLQIRGVLLAQQNAQAVKDAA
                     SANKEAIQTAGDEHFRSGAYHKSSGKKW"
     gene            complement(17351..18595)
                     /locus_tag="VAH76_000883"
     CDS             complement(17351..18595)
                     /locus_tag="VAH76_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEO1385530.1"
                     /translation="MNKNNDYLSPDNSPQKLQTAGVKRVNNVPLMIAIGILTLFVVLI
                     ALVANKRANAQNQPPEIVKIKNPKKNTMSLANDVVGNHKSAVIPPQSVTIANNEAAPL
                     PIPEQLLPKEDVLTQDVSEAEIERIRQEKTQEFEEAVKAKTSVMVDNPRLNNRDTAKT
                     SMNSNEMAINIDPSLAFKEQLALLQGRQAKPMPQTLGGEENEMRWHLNSTLQSPNSRY
                     ELRAGSVIPGVMISGISSELPGQIIAQVSQNVYDTATGKYLLIPQGTKILGLYSSDVP
                     FGQNSVLVAWQRLVFPDSKALDIGSMPGADSAGYAGFRDQVDHHYGRIYGSALLMSGI
                     IAGITYSQNTNQSSQFGYAQPTAGSVMSQALGQQLGEVTSQLVSKNLNVSPTINVRPG
                     YRFNIIVVKDLTFKQPYKQFAY"
     gene            complement(18601..19008)
                     /locus_tag="VAH76_000884"
     CDS             complement(18601..19008)
                     /locus_tag="VAH76_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HEO1385531.1"
                     /translation="MKKLSVLLAAVLLSSCASMRYGNFTQMSQGKDAYLAADAATQLT
                     RVYPPAQNTFCIGQAVNDGFGLSLIQNLRKKGYGINENQCPKNKANFFYVLDELKPQS
                     YRVSLFVGMQTLSRLYAKSHENIIPISTWTHKE"
     gene            complement(19011..19886)
                     /gene="trbG"
                     /locus_tag="VAH76_000885"
     CDS             complement(19011..19886)
                     /gene="trbG"
                     /locus_tag="VAH76_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEO1385532.1"
                     /translation="MKRVMQLFCLLTPLTSFALDNTILAEHYFSDTVPKLSSQEKQGL
                     DIAERTQQGEKTSIPFASADGSVSFIYGSGQIRVVCAPLQVCDIALQPGEQFNDMNVG
                     DPRFVVEPSLTGSGAMQQIHLLIKPKDVGLDSSLVITTDRRTYHFRLKSDRTEFMPYI
                     SFSYPDEAKAKWQLLERIQAERRKVDTLPETNEYLGDLNFNYRIQGNARFKPVRVYNN
                     GVKTIIEMPRTMAQSEAPALLVLRSRGIFKKSESVMVNYRLQGCRYIVDGVFDKAILV
                     IGSGSSQEKITITRC"
     gene            complement(19883..20629)
                     /locus_tag="VAH76_000886"
     CDS             complement(19883..20629)
                     /locus_tag="VAH76_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HEO1385533.1"
                     /translation="MAFRKRYSNGTIRRRSMKKSVTNNPYLNARRAWNVHTAGLMKSL
                     QVWQLVGLGSLLIALAAVAGLIAIGSQSKFIPLVFQQDANGNTLSVTRADKVGEASID
                     DYRAAAAHFIENVRMVSLDAELQKKAVFQVYSYLNANDAALAKVQEFYSDKQQSNPFE
                     RAAFEIVSIDIRSVLQESDETWQVDWIETVRNRDGSLKEKPRMMKAMITMYQENELND
                     ATSESILKNPHLIYVRDFNWSYELKHGEQQ"
     gene            complement(20578..23112)
                     /locus_tag="VAH76_000887"
     CDS             complement(20578..23112)
                     /locus_tag="VAH76_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869966.1"
                     /note="type IV secretion system ATPase VirB4 family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HEO1385534.1"
                     /translation="MIGFITFLISITGLLLLGTLFSAARMKSRKNHVRRHRSHTAGLV
                     DLLNYAAVVDDGVIVGKNGSFMAAWLYQGEDIANTTDEAREMMAFRINQAMSSLGNGW
                     MIHVDAIRRAAPGYSEQSASYFPDPVSKAVDEERRLYFESIGALYEGYFVITLTWYPP
                     VLAQKRFVELMFDDSGEKLNQKAKTHQLIETFKQSCRNFESRMSAALHLERLGSELFI
                     NENNKVTQDNFLRHIQYCVTGINHPVNLPKNPIYLDALIGGQELTPGITPKIGRKFIQ
                     CVAIEGFPMESYPGILTLLTQLPVEYRWNSRFIFMDPHEAVAHFTKFRKKWKQKVRGF
                     FDQMFNTNSGVVDEDALGMVNDAQAAIAETNSGMVGQGYYTSVVVLMDEDRANVEKAA
                     LFIEKNINALGFSARTETINTMDAYMGSLPGHGVENIRRPLMNTMNLADLLPTSSIWT
                     GENKAPCPLFPPQSPPLLHCVTSGNAPFRLNLHVRDLGHGIMFGPTRSGKSTHLGLLA
                     LSWRRYKDARIYSFDKGMSMYPTCKATGGEHFTIADKDSRLAFAPLQFLETKADRAWA
                     MEWIDTILALNGLNTTPAQRNEIGHAILSMHQSGSKTLSEFVMTIQDEPIRETLKQYT
                     IDGLMGHLLDAESDGLGLSSFMTFEIEHLMGLGEKFALPVLLYLFRRIETSLDGRPTL
                     ILLDEAWLMLAHPVFKNKIAEWLDSMAKKNCVVFMATQHLSHAANSGILDIIVESTAT
                     KIFLPNLYARDPETRTIYERMGLNPRQIDIIAAAQPKRDYYYVSEKGQRLYQLALGPY
                     ALAFVGATDPDSIARMKQLELKHGEEWVGQWLSEKGIPMEQYGEEA"
     gene            complement(23109..23408)
                     /locus_tag="VAH76_000888"
     CDS             complement(23109..23408)
                     /locus_tag="VAH76_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HEO1385535.1"
                     /translation="MSLRTIPIRRCGNRPSLFMGGDRELVMFSGLLSAILVFAAQDWL
                     AAIFGFALWFLSLKGLRLMAKADPYMRAVYLRQRGYQAYYPARSTPFRNNKGGYL"
     gene            complement(23405..23782)
                     /locus_tag="VAH76_000889"
     CDS             complement(23405..23782)
                     /locus_tag="VAH76_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEO1385536.1"
                     /translation="MNQAININYRSLWMIGAIVLLLLFITDPACASSAGGGLPFDSYL
                     TKIQKSITGPFAFTAAIIGLVGAGATLIFGGEMNGFLRTLLFIVLVLSFLVAAQNTMT
                     AITGKGAEIAKPSVLTSATELQP"
     gene            complement(23794..24759)
                     /gene="trbB"
                     /locus_tag="VAH76_000890"
     CDS             complement(23794..24759)
                     /gene="trbB"
                     /locus_tag="VAH76_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HEO1385537.1"
                     /translation="MNAQEEQGITVKDRAREKLRRDLGGMIEQALSDPKTVEIMLNAD
                     GKLWQERLGETMRCIGSINDARAESIIKTIAGFHGKEITRFNPILEGELPLDGSRFAG
                     QLPPVVSKPTFAIRKKAISVFTLDDYVKSGIMTEAQCAVIKKAVIAHRNILVIGGTGS
                     GKTTLVNAIINEMVCSSPTERVFIIEDTGEIQCAAENCVQYHTTIDVNMTQLLKTTLR
                     MRPDRILVGEVRGSEALDLLDAWNTGHEGGAATLHANNCLAGLHRLKSLITRNPAAPT
                     EIEPLIGETVHCVVHIARTPQGRRVEEIISVKGYENGHYNIEQLI"
     gene            complement(24788..25048)
                     /gene="csrA"
                     /locus_tag="VAH76_000891"
     CDS             complement(24788..25048)
                     /gene="csrA"
                     /locus_tag="VAH76_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEO1385538.1"
                     /translation="MLVLTRKVGESVVISEEVYCTVVGYRDGNVRLAFDAPQSIPVHR
                     DEIQRRIYHERQKDQWFSDFPSNKESIVDRLISKFKNGLKSA"
     gene            complement(25066..25377)
                     /locus_tag="VAH76_000892"
     CDS             complement(25066..25377)
                     /locus_tag="VAH76_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HEO1385539.1"
                     /translation="MFTRFAVYYGHLWRSQFKNDGFLEFAKKEWLEGLSQFSDEILNQ
                     AVIDCRDHCEMPPTLPQMIGFCRDIKRRNAFNVRPEKYQPASKEVVEENIRQCKAYLF
                     K"
     gene            complement(25397..26281)
                     /locus_tag="VAH76_000893"
     CDS             complement(25397..26281)
                     /locus_tag="VAH76_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385540.1"
                     /translation="MYFTVNDNELDALCGLSYIQQITYLRGIRPYMDRNTFIVGIKRR
                     ISYQSLAETLYIEPHQGITESGSPSRQQIRRVIKGLERAGLIVIQSLDKHLILKCLLA
                     DISYCVQNKPGTNPTQQADTKPHATPPVNTGYSERVCEKADTNEIIKADTPLIKENNY
                     IYLLQRFDHFWRMYPEKKSRERAFEIFTQINPDEPLLQCILQALYQQITAREAKEAHG
                     EWVPAWKFPANWLSQKCWEDEVKIELTQEKRNEKRGTNTKSGAIDPFWNPETGETAST
                     DEDEYKQSNVINLQCYRQ"
     gene            26483..27166
                     /locus_tag="VAH76_000894"
     CDS             26483..27166
                     /locus_tag="VAH76_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1385541.1"
                     /translation="MNIKEKIGQRIMNERKAKGLTRKALAELTSELKISRINNYERGD
                     RTPGPTEIKLLADALEVSASYLMCLTDNREGKMTKSLGMGALIPVLDYKQAIDSATFI
                     QKIKEDVDTKVEFIPVSTAVSDSIGKNAFALQIKDESMMPEFRISDVLIVDPDTSPKP
                     GDFIVALIEGDQEVIVRKYKQLSASKEAHQFELIALNEDWADIRVGSSEMQAQIIGCG
                     VSLVRGLKN"
     gene            27502..>29340
                     /locus_tag="VAH76_000895"
     CDS             27502..>29340
                     /locus_tag="VAH76_000895"
                     /inference="COORDINATES: protein
                     motif:HMM:NF016724.1,HMM:NF022063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO1385542.1"
                     /translation="MPFDPTILTESNLNRYDKQLERISNDSGYTENIQETVVNAIDNI
                     TTGCKSFVIYGEPQSGKTEMMIALTAKLLDEENKIIIILLNDNVQLLNQNLDRFKRSG
                     INPTPRTFDEVINQVTDIRNSEWIIFSKKNSKDLQKLLTIVGSEKNKVIIDDEADYAS
                     PNAKINKQEQTKINELVEKLISDNGIYIGVTATPARLDLNNTFRNENQKWVYFHPHEN
                     YCGHDIFFSPAEPIGNLIKLIPDSEDSKKSLSTCLFSFLINIAYLNLRKNSNEENYCM
                     LVHTSGKKIDHIEDQKNLEKIFNILTHQSDSKFTKYYEKIWEITNNKFNCDEELTQEI
                     TKYIRNNIKRYQIIVINSEHDKEKVKGATRPSSLFTIAIGGNIVSRGVTFENLISMYF
                     TRTTKHYIQQDTYIQRARMFGYRKGYLGQFELHIPEQLYKEWQKCFVYHRLSLELIKT
                     GRGVPSWFEDKRVTSVAKPSIDKNTVLLYHGEMAFAIFKYTNEIESIINKESHFYFEK
                     LTKLHTIIGEKSLPMCLIDFIHRTSNGHNDKIAIHNPGSVSNRRTVDPITITRTGGGM
                     LGTGDLELNKFPHAYHHIKIFYTENGDAKLYYKYQRERIKIRRNVGN"
BASE COUNT         8702 a         5905 c         5739 g         8994 t
ORIGIN      
        1 taatgttaac tataagcagt gaatagtttg gctctaagag tgtttgcgtt gagccaattt
       61 attcatgtgc actcaaatca atgtgctatg ttctcatatc tttcaattcg taaagacact
      121 ctgtaaacta attttttacc tctttttgca aaagcatatg acaaaatccg attcttttta
      181 taatttggag actctaggcg gcattttatt atttatcgca gcagtcatag cgatagtagt
      241 cgccaactct ccctatcgtc atatatacga tcaattgctc catattaaag caacggttgg
      301 tgtcggctcg ttccttataa aaaaaccatt gttactgtgg attaacgatg gcttgatggc
      361 catttatttt atgttaatcg gtcttgaaat aaaaagagaa attcgacgtg gtgtgttaag
      421 ccataaaaag aatctaattg ttcctattat tacagcctta agtggtttac tcgtccccgc
      481 tttaatattt tcttggttta atgttcataa tcctgtttac ttgcaaggct gggcaatacc
      541 tactgccact gatatcgcat tcactttagg aattgtgtca ttgctaggct cacgtgtgcc
      601 gctgtcttta aaaattctgc tgacagcaat tgccattttt gatgacattg ctgcaattgt
      661 cattattgct ttcttttata ctaaaaacct atctttgctc gcactttctt ttgctctatt
      721 atttaccttt attttaatag gcttaaatta ttttaaatgt cgccgggttt ctttgttcat
      781 agtggtaggc atcttcttat ggattgcagt attgaagtca ggggttcatg ctacattagc
      841 aggaatagtg attgcgatga ctattcctga tgagagcagg gggtcgatgc ttgcccgcct
      901 cgaggatggt ttacatcctt ggatagtgtt tttaattctc cctgtatttg cctttgccaa
      961 tgcaggcgta acatttttgg ggcttgatgc atcgatgttt attcacccta ttgtgctagg
     1021 aataggatta ggattattta ttggcaaaca aatagggata tttttatctt taggctattt
     1081 tgttaaattt aaacacctcc ttaaggccga taacattacc cttggccaag tttatggcat
     1141 tgcgttaatt tgtggggttg gtttcaccat gagcttgttt attggctctt tagcctatca
     1201 gcataacgat ttgaatttaa tgcctttagt taaaataggt gttgtccttg gctctttgac
     1261 ctctggaatt ctaggattta tagtattaaa gagggcttca tcgtaagtca ccaattgcaa
     1321 acagattatg gattaacact attatcgtta tggtttattg aattggcttc taatattgaa
     1381 taagtgattg acgaaacaac tattataggg acgaaaaatg aagtcaagat tattaaactt
     1441 tctatcagaa actcgagcaa gttattggta cattcctttg cttatgtcca tagccgcttt
     1501 attgttttct attctatccc tgcgtcttga ccatctcttc gtcttgcatt ggttagatac
     1561 atggggttgg tttcatgcga gtaatcctga aggggcaaga gcaattttat caaccattgc
     1621 tacttccatg attacagtcg ccggagtcac tttttcgatg actattgtgg ctgttgcctt
     1681 tgctgccagc caagtgggtc cgaggcttat ctcaaatttc atgcgcgata gggcaaatca
     1741 aattacacta ggcactttta tcgccacctt tttgttctgc ctgttcattt taatggctct
     1801 ttacaatgcc aataaagttg gggttagtga atctgatgga ttggttttta ttccccagat
     1861 atcgttactc atggctattt tctttaccat aagcagcatc atcgttttaa tctatttcat
     1921 tcatcacatt cctgaaagca ttaacatgtc caatgtgatt gctcaaatcg gcgaggaatt
     1981 aaataggcag gtggattgtt tattcccgat aaatgtagga aaagaaatgt ctaaacaatc
     2041 cgctcatatc cccaaaaacc atcaacatcg tgacgctgtt gttgcaacaa aactgggtta
     2101 tgtccgcatc ttagatggga acacccttat ggctatcgca aagaaacatg atttaatcat
     2161 gcagctggaa gccggatatg gcacttatgt tactgaaggt tcaaccttac tctatatcta
     2221 ttcttcagaa aaaattgaga agcttattca taaacaatgt gttgatgctt ttgctcttgg
     2281 acatcagcgt aatcaggaac acgatattat tttcctaatc gatgagatgg tggagattat
     2341 agctcgtgct ctatcacctg gtattaatga cccattcaca gcaatgacat gtctggattg
     2401 gctgcaatca actttgcaaa aaatatcaaa aaccagtcaa ccctctgcgt atcgctatga
     2461 tacagaggat aaattgcgat taattgctca acccattgct tttactgaat tttgtgactt
     2521 gattttttgc agagtacaac cctatgtatg taaagacaga aacaccgcaa tccacatgat
     2581 ggagatgatt aacaccattc atgcccgcat tactaatgat gagcataaaa ttaccttaac
     2641 atcgcatgcc gcatcgctca aaaacgcagc acttgaatgc ctgtcaatca atgaggatcg
     2701 aaaaaagctt caaagtctgt atgaaaagca ctttgttaat tcattgtaaa agatcattct
     2761 ttaatcgaag gaaacaaacg gattggtccg cgtgttttaa gctaaatgac ctcactgaat
     2821 atgccagttt ctgtaaaagt gaagtattgt ctgtttagag taatcgctac cgtaatttga
     2881 tttattcaat gccgagttta gcagtaatat cgattattcc ccagccgttt ttttggcaaa
     2941 atagaactat aatttatgta ctaccactat tcacggaagt aataaatgac taaaacacta
     3001 aaatatttaa tgcatgagct taagggaaag actcttttgg tttcagtcgg tgatgaaaaa
     3061 tttgatgtag atttaaatga ttttgattct tattcacatg aaggaatgac ttttataaag
     3121 gttactgcct cagggaaaac tcgatatata aatcaggcca aaataaaata ttttgagatt
     3181 acctaaaaaa agcagtccag gatcctcaca ttaaaacaca aaatatcatt caatcatcta
     3241 tactgtacaa attaacaatt tagtttctac tggagtaaca ataatggact tacttactca
     3301 agatttccct gacacatttc ttaatccatc tgaattaccg tgtatttctc tttatcagcc
     3361 cacccatcga agtcaccctg ataatgagca ggatgtaatc cgttttaaaa accttacaaa
     3421 agaaatacag cactcattag aaaaaaaata ttcgaagagt acaattcaat ctctactgaa
     3481 accatttcat tccttagcgg aagatttttc attttggact cacaatagag atggtctggc
     3541 tattttagca aacccgcaat tgttcaaggt atacaagctt agccacccag ttcctgagct
     3601 cactgttgta gcagatagtt ttcacattaa accgttgata cgaataatgc agtctgcaga
     3661 tcgatatcaa attcttgccc ttaatcgtca acagataaaa ctttttgaag gaaaccgtaa
     3721 tggacttgaa aaaatagaac tagaaaacga ttttccaagt acgataacca aggctttagg
     3781 taaggaatta actcagccat acgttactgc agggagttat ggtatgggga ctgaaggtcc
     3841 ttcgatgcac catggacagg gtagcaaaaa agatgagatt gacgttgatg ttgagcgatt
     3901 tttccgtgtg gttgacgagg ccattctaaa gtttcattca gagacttcaa agttgccttt
     3961 actgctggct gcattaccag aatatcataa tctttttcat aaaatcagca aaaatccttt
     4021 tttaataaaa aacggcatcc aaatcaatac tgatgattta tccatagaag agctctgtaa
     4081 acaggcttgg aaacatatgg agcctcatta tttggaaaga ctggatacat taaataagca
     4141 attttatgaa gcacaatcta acgaattggg gactgatata ctgaaagata tagccaaggc
     4201 agcggtttct ggacgaatat caacattatt ggtcgatgca gatcgtcaga ttgcgggcca
     4261 aattgaccat gaatcaggtg atataaaact tgatgaactc gtaaatcctg atattgatga
     4321 tcttttggat gatttaggcg aattagtatt aaaaacaaaa ggagaggtta ttattctacc
     4381 tagcgaacga atgcccacga atactggagc cgcagccatt tttcgctatt aaggcctggt
     4441 agtagcctgt ctgtagcgag ttgctaattt ctaataaata tacctgattt acaattcgct
     4501 aaagacagga tcaaaaagga acttttgttc ctttatccca caatatattg cacttttctt
     4561 ttgccgcaca ggaaaataac tcaatcaacg gaaaagcatg tgtttccaag ctcacatctt
     4621 cgtcctcttc ttcactctgc gggttagata tagtcagttc agctgagtta ttttcctgct
     4681 taaatgcact ttttaatcga gtaagagctg cagggacatc tttagctata atcgcaccag
     4741 gtacagaacc actgtgcccc attattttga gcagtggaat agcaacgtca tctagcattg
     4801 taatatctgg atagacgtcg catctaaaag ttaccagcat aaaaatttcc tattaaaaat
     4861 atcttttgaa taagtatagt cctcttctcg tattaattaa tttatctatg acttttggag
     4921 caggtttaat gacaactcca aaaaattttc ttatcataaa cttagcaaga aactatgtta
     4981 acgttcactt tgcagcaaaa aaagcgttga ttcattattt tcactaattg gtgagtagat
     5041 ccactgcgtc gccccagtga acccctctcc agttgcataa ctataaacat cctataatat
     5101 ggtttatatt catgattatc ttaaaaaatc aataagaaag agcaacataa aggcacaagg
     5161 tacagtcagc attgcaaccg atgataacaa tgtaaaccaa tttgttttcc gcgtttgttg
     5221 agtcaaatat cgcgcttctg tcagtgactc tgatatcagc cttttcatgg catgaacaga
     5281 ttcactagtg gtttctctca atattttgtc catcgcctct ttactgcttg ccaatgcagc
     5341 attaagcact ttctcggctt tttccttagc atcatccttc cactgagagg aaatgtcctc
     5401 catttcctct ttaaagtgag tcagcatatc ctgttgggcc tttcggtttt cctcaatcag
     5461 cctctcgttc atcgtttgga gaatgagaac cggatcatct ttacccaaaa ccacaccatg
     5521 tttgactgca atgtcttgaa tggcttcatt aattttaact gacattagat caccatggcg
     5581 ttatcaatct ggctaaatag ttgatctcgt ataagcttta atcgctgcct ggtcataata
     5641 tttcgatcag gggagtcgat tgcttcatta aatgtaattt tttgttgaag catgtttgac
     5701 agatctaggc cgaaggtttc ttcctttaag tcagggatct tgattatcgc cgctattctg
     5761 tcttttgttt ctttgtatac cctcatttgc tcaaatgacc ttccttcact ctcaattttt
     5821 ccccaatagg gatttagcca tacaacaaag cgaacatctt gtggaaattg gttcaccagt
     5881 tgagaaaatc cgtgtgttgt atccagtagt gcttgaccac cggtgataac ggtatggatg
     5941 atgagttcat gccccagtcc ttttaacaag gtcggcacct ggttggtgag gaggtaatgc
     6001 gataaaggaa caaatgagct ggcaccatta tcaataatca catcattttg tgtgtttgca
     6061 attttttcaa ttaaagcgtc aaagtggcgt gggtttattt catctccatc cataatatcc
     6121 aggcgttcga cgttaagtga tttaaaacca tgaaacgtgg cattaatggg atcggtatca
     6181 aaacaaagtg gtggttgttc tttgtagagt ttgtattgag cagtggttgc tgagacaaag
     6241 gatttgccta caccaccttt gccttgcatc gtaatatgta tttttgccat tacaagagtt
     6301 ccttcgggtt gggtattgga ttaaaagtaa aacctttgat ttcatgtgtt gttttacttt
     6361 tgccctgttt ctcatccttg gtgttttcct gcgctgactt aattaactta agtacgtaat
     6421 gtctaaatgc tttataacca aatgaaatat gaccttcatc agacaaagtt tcccaaacag
     6481 ctttcatcga acatccctta tcaagggctt ctttaatgtc ttcttggagt gctataaatt
     6541 caatctttgc actgattcgc ctggcctttt tttctgactg gctctggatt acccgttctg
     6601 aaagtggttt tttcattctt ctttggcgtc acattttttt atttaaaaag tctaaatcat
     6661 aattcggttt ggaacaaggg gaaaaggaat tttctggaat tattaggagc ttactcgaat
     6721 acataaaggg ttttttaggt cctttaaagg aacttttatg aataatttgg atttatttgg
     6781 tcccgtggtc gaaaaagttc tataaaaagc gttgcaagtt ccaaaaagtt cctgctatga
     6841 ttccaaaaag agatctcaaa acgcagggca agataggtga agttagctaa ccagcaagct
     6901 ggctaccaaa cctcacaagt cttattcgca ccttcggtgc tcaacgaaaa aactgttttt
     6961 tgagattaaa tcaataccca aaggattgca taaccactta atcaagaggt cagcatcctt
     7021 atggacgaga aacttaagac acccaccaga aaaaatggtc gccatcttcg cgtacctgtc
     7081 ttgccttctg aagaaattca aattaaatcg aatgcagcca ctgccggtct ttcaattgcc
     7141 gagtacttaa gacggataag tctcggttat caaattcaaa gtagtattga taaagatcat
     7201 atactgcagc tagccaaaat taatggggac ttaggacggc ttggtggact gttaaaacta
     7261 tggttgactc aggataaacg cgtcgcgcat tttgacctcc aaacagtaaa aacattatta
     7321 aaccgtattc aaactaccca ggatgcaatg ttggaagtgg tgaaaaaact atgattattc
     7381 gccatattcc tatgaagaag acaagactta gtagtttctc tggtcttgtt aaatacctgt
     7441 gtaatcagca aaacaaacag gagcgagtgg gtaaagtcag attatcaaac tgtaatagcc
     7501 tggatcccat ttgggctgtt caggaggtat tagcaactca agctaaaaac caaagagcca
     7561 ctggagataa aacctaccat atgcttattt ctttcgctcc aggagaaaac ccttcccctc
     7621 aagttttacg tgaaattgag gatagagtgg cattgtcgat tcgacttaaa gaacaccaaa
     7681 gaatctcagt tgttcatcat gacacagaca atcttcatat tcatgttgcg attaataaaa
     7741 ttcaccctca aacctttaat atgattgaac cttacagggc ttataagacc tttgctgaag
     7801 ttgcatccca gctcgaaatt gaattaggtc ttcaaataac taatcatcaa actcggaaaa
     7861 accgttcgga aaatcttgca gacgatatgg agcgccattc aggtattgaa agcttgatta
     7921 attggatgaa gcgtcactgt aaagaaaaaa tagaggcagt tagaagctgg aaagagattc
     7981 atagtatttt ggcagaacat gggttgatga tccgtgttaa agccaatggt tttgttttct
     8041 gcaatgtcca aggactcacc gttaaagcaa gttcaatttc cagaagcttt tctaaaaaaa
     8101 atctcgaatc caagctaggt tctttcatag catcctactc agaaggcgat gcacctgagt
     8161 ctaatgttta ccgttatgaa ccattaaata aaaaggtact tggctctgag ctctatgcca
     8221 catataagca tgaaaaatca cacaataaac acattatttc agacaaactt aaaaacttgc
     8281 gagaggccag agccaaactt attgaaaagg ctaaaaagcg aggtcgaatt aaaagagcgg
     8341 ctttgaaatt aatgaacctt tctaaaatac aggaaaaatt tctatatagt cacatcaaca
     8401 aaacgttact taatgagatt gatagcataa gaaaaaatta tgccaaagaa cgaagcaagt
     8461 tattagattc ccaccggaac aaaatctggg ctgattggtt gaaacaaaaa gcacaagagg
     8521 gcgatcagga ggccttagtg gcaatgcgtt atcgtaatcg taaaaataat catgattaca
     8581 ccatttcagg taaagactca gtttctacta ttttcaattt tagaaaaatc gactctgtta
     8641 ccaaagaagg cactgagatt tataagaaag ataagagtgt tattagggat aatggaaagg
     8701 aaattttcat ttctaaaggt ggttctattt cggctcttaa aagagcgttg gatatggcgc
     8761 ggcagcgtta tggggactgc atttgtgtga acgggtctcc actatttaaa aagattattt
     8821 tgcaaattgt cattcaatac caaatgccga tcacttttgc tgatttggat ttggaaaatc
     8881 aacgtcaaaa actaaacttt gagcaggaga aatctcatga acaatcaaga tcaagtggac
     8941 aaaaccatcg aggaagaact tcaggaggct atgagactat tggagcagcc aatgaaaata
     9001 gaaagagccg aggcacaaag cccaacactt tcagcattag acaaggctcg ccagccaaag
     9061 atcacaacgg cttgcgaaac ttgtcccaat gcgatctggt tcagttcacc agaagaggtc
     9121 aagtgctatt gcaggatgat gcacatgatc acctggagcg gcaaggattc aaacctgata
     9181 accatgtgcg acgggaagtt tctcgattaa agaagaatag gaagggttat aagaattgaa
     9241 atctctctga gacttcaaac caacaatttc cttactattt tgttaggctc tattgaaaat
     9301 tgtttatatg tttttattgt cgtctctgtg tcgtgctact gtcgtggttg acaaacctta
     9361 cattgataca ataaaagctt atttattaac cagtgaggta aggcaatgac aaatgaacaa
     9421 aataatggca catacggtcc tataccagca aaatatcttg ctttctacat tgtactgtac
     9481 cgcaaacaga gaggatggac gcaagaaacc gtggcagaac tcacgaaact taatgtgagg
     9541 acgattcagc gagttgaaaa tggtaaaggt tcgagtccgg atgtgagaag agctctggcc
     9601 agtgtttttg ggcttgaaga tattgatata tttaacagac cctttcaaca tcccgatgaa
     9661 gtagctctaa gagaagagta tgagcggcta gaaaaagaaa ctatcacact ctctgtcaag
     9721 aaagtcacat gcggcaagca gcttcgagaa atggctgaag aggcacaagc acatcagttt
     9781 gaagcaagag aaggtctttc aaaggagtct gaacactgtt ttgctgagct tcaagattat
     9841 cttcaggatt gtgacgggat atatcaggaa atgacagcag tacagaaatt ggaaatcaat
     9901 gaagaacttc agagtatgct tgaacggttt aaaaatgcag gagtgagtct tggtattgcc
     9961 gttggaagtc ttaaaatggg ggatgataaa gatccttttt ccataagaag caattgttat
    10021 attgctgcac ccaatgacag ctttccagaa aaaattatgt taaataaaag tgtacgtatg
    10081 agtatgtaat ccattgagaa gtgtcttacg tttaacccag gtcggatgat tataatacat
    10141 ataatatgaa ttgaattagt tttgacttaa tggcgatcat cgaatgatcg ccattgaaat
    10201 tgaatttatg tgatacgaac cacattcgac ttttgcgtta attgcaggcg actgttttgc
    10261 ttagcaacac tatcaatttt tgattttaac tgccgtttca ctcccgcaat tcccagctga
    10321 cttttgttag ccagatcatt aaaatcgcta aactgtcgag ggtttgataa ctgctctcca
    10381 ggagcaaaag tcggtagaat catgaaacca ttaactgaat ttgccgcttc actacctttt
    10441 tcttttccag gattgatgcc ctttgttaac tcaagatgtt tgtcatcatc tgaagccaca
    10501 ataattggtg tattgggaaa tttatcacgt aaagctttag cgactggctt gaggttaccg
    10561 gaatcaaagg cggagacgat agctggtaat ttggttgcat cttttatagt tgcagcagta
    10621 gcgtaaccct ccgtaattac aataacaggt gtttctgcta gtttttcgag accacccaat
    10681 acatgaaaac atccctcttt ttttgagtct ttcgcaaagc gtttggctcc atcttcagaa
    10741 atgtactgaa tagtccaaag agttccttcg caatctgttg cagggatgca ggttagtcgt
    10801 ttctcactat ctgtatatac tccgtaatga ttcaggatac ccttagcctg catataaggc
    10861 gtgggttcca caacctctgt catcagggat aatttttgct tgagttttaa tgcagtactt
    10921 ttttgtttgg attccagctc acgttggcga atttgttgat gttcaagcgc ttctgcctta
    10981 agcgttgact tctgttcgtc agttaaaatg tagcctttgc atttccagtt aagctcagta
    11041 ccagttcggt tgtttttaat atatcctgct gggataccgt ctaaatgggc tacataaaat
    11101 cctgctttct cactattttt atcaccctca gtggaaatac gatgtggttt accatccata
    11161 atcggatgct cacctgaaac gatagcgcca aggcttatca atgccgcttt aaattcgtcc
    11221 tcagggataa gcgctttttc ctgagttaat acctgatgct caggaagcca ggctttgatt
    11281 ttctcaacag aaacgccagg gtttgcatac caacattttt gtactttatc ccaggcaata
    11341 cctggtgttc catcagggag ttttcctata gtggatttgg caatttcttt ttgttggaag
    11401 gggatgctta gccagacctt ttcggaacgg atgttttcag acgccataac gacttcctct
    11461 ttgagtttat catccttgat ttcaacaagt tctcccagat taatgttttg ggtttgctct
    11521 aaagcacata agtgattcac tattttctct gcatctgcgg atgccctgaa tatttctaaa
    11581 ggatcgtctt ctaaaactcg tatccatgat ttgatatatg cagtgtgttg agaagggtca
    11641 tggcctatac ccagttcggc acctaacagc atgcttgcaa tttctgctct taattcttct
    11701 ttggcataag catctgaacc aaagggatgg cctaaatctc tatccaatct tgagggatga
    11761 ccgctccaat gccctagttc atgtagggca gtagtatagt agttggccgc agatttaaat
    11821 tgctctttag ggggaagatg aatgctatca gtcgataatc tataaaaagc tttgtctgct
    11881 tctgagtgaa caattttagc accagagtta tgtagtagtt tttcagcttt ttccattaag
    11941 gaccaatctg gctcttttgt aatgagttct ggcatattat caatttgcga ggcatgaaat
    12001 accgttgcat aaaacacttt gggtcgttca agattcacct gcacttttaa tggattaccc
    12061 tgttcatcaa gaacaggttt accagtatca tcatgtttgg tttgttcttc atgaaatttc
    12121 caatattgga ttgtggtgcc tttttctccc ttgcgaactt gtgcatccat actttgtgct
    12181 tgtttgtagg ttaaccaacg attgtcatca ctttgattca ccattaaata caaagcattg
    12241 atccctcgat aacgcttccc ggtgactgga ttatatggaa tttgaccatt gccaacacct
    12301 ggctcccatg gttttaacca gggagcggtt cctgctttca ggctttcaat aatttgattg
    12361 gcaacgactt catggtaagg cattttagtc ataaatagcc tccatcgcat cttcttcatt
    12421 gagtgcatct tctacaaatg ctcccatgat gtccgcttct atcggactga cttcaagaag
    12481 aaatccaggg attactaaat cctcaaattt ttctgcaatc acgtccatta actcggtttc
    12541 atgattcatg tggttctctc cgtttattaa ttacccatat gggtgtgatc attcctttga
    12601 gttgtcctgt gtgaacaaga ccataatatc gaccatcaaa tgaccattcg ctttgactgg
    12661 tcatagtcat cacttcatcg ttttggagtt ggtaattcat tacttgccat tgaggtaagg
    12721 agcgcttcat cccatcccgt ggttttggtt ttgagtaggg ggttagtatt tgattaacaa
    12781 aaaccccttt attggtcacc gagaggatat cgccagatac agccactact tttttcatta
    12841 aataaccata tccatcacag aaaagaccat ggtctatata acctctatct tttgctaacc
    12901 taaacgattt cctgctatca ggacaaaaaa tcacataagc atttttgata ggtgcttcgc
    12961 cggtgatacg atataaacct attggaatgg attcagtcag atttatccta aaacccatag
    13021 tatgaaatag tacgcataaa acaataaggc ttatcaaaaa catagaggta aagattgaca
    13081 gttttttcat agcctgatga cctcatcttc acttaaacga gtacgtaaaa tatctgagtg
    13141 agcaggagca tcaactgatg ctcttgtcat gaagatcgga tctttaaagt aaagaggttg
    13201 tttaccataa atagcaggga agcccgctgc atatacgacc atgtcgcctg cttcaacaat
    13261 caggccatta gcatcttttt taggtcctgg catacgcaga cactcatcaa cggttaagag
    13321 cggtctcgat atttcctgaa cggtttttga gatttgattt aagaacgtag aaaagcgttt
    13381 tccactggtg gtgatgtgtt ctttaacaat agttgtttga cctgtcagct tggaaagatg
    13441 ttctgcggtt tcaactctgt tgggtggata agcattttgg atatgacaat ttgaagtgat
    13501 ggtttcatcg gggccataac ctcgctcacg gcttttcagc tgattaatgt cctgacaaat
    13561 taagtaaaac tttaaaccat agcctgcaac aaaagctaat gattcttgca gtatgtcgag
    13621 ttttccaaga cttggaaatt catcaatcat gcaaagcagt cgatgcttat aggttttttt
    13681 agcacttaca taagataatc ctctggcatc ggatacacgc tcaaattcca ttctgtcggc
    13741 caatagtctc actatcatat taatcatgac tctaaccaga ggctgaagcc ttgccttgtc
    13801 attgggttga gtaacgatat agaggctcac aggacattca tggttcatta aatcgcggat
    13861 gcaaaaatct gaggcagaga cgttatgcgc aactactgga tcacgatata gcgctaaata
    13921 agattttaaa gtagataaca cagaacctgc ttcttcttcg ggcctatcaa tcatgtcacg
    13981 agcagaggct gatataacag gatgggtttt accctcaaca tgcggatatt gggtcatttc
    14041 aatgagcagg tcagcaatat taatattggg atcaaccagc atgcggtcaa tgttgggaaa
    14101 tgtggctggt tctccttggg ttttcaattt ataaatcgca tgcaggataa aaccaactag
    14161 aagggcttga gaggttttct gccagtgggt ttccaaccct ttaccatctg gatcaaccac
    14221 aagcgttgcc aaattttgta cgtcacccac ctctgattct gtatccactc tgatttcatc
    14281 cagagggttc cagcgtgcgc ttccttttaa cgttgcaggt tcaaatcgaa taaccttgtt
    14341 ggtggcatgt ttttgccgcc atccagcagt cattgcccag agctcgcctt ttaaatctgt
    14401 aataacacaa gagtgtttcc acgatagaag agttggaatc acaaggccaa cacctttccc
    14461 tgagcgcgtt ggtgcatacg ttaaaacatg ctcaggccca ttatggcgta gataatgaat
    14521 ggctcctttg tcatcttcaa acgcgccaac ataaactcct tcatcatgtc caatgagact
    14581 agcactgttt aagtcttccc aattagccca cctggctgaa ccatggaggt agtcactcac
    14641 actgttcttt ttaaaatact tggaagccaa cacgatcatc attaaaaaga gacaacccat
    14701 cataacgatg agaccaaagg cggcattaaa agaatctggg taatagggtt gatacttaat
    14761 agcccaggta aaaatctgcc aaggcatata aataggggcg aatgtccctc caagggcgtc
    14821 agcatagtga aattgatgcg cgaagaattg agttccaatg tgcatgctga ttaagaaaga
    14881 acaaatcact agcaagacta aagttctatt gatcttgctg tgattttttt ctctgacttg
    14941 aggacctatg gctttattag gtttgttttg gctcattggc tctctccaga tgccttttgg
    15001 ttcacaaatt gactgacctc atctgatgtc tcaaatcccg aactgagact acctgcaggt
    15061 gtgcccatag ataaaatgtt ttgcgcgtta gattcttgcg attcagaagt ggatgtatct
    15121 accttgttat gaattgcttc ggcaattttt ccacctgttg tgtcagcgat tttttcagca
    15181 actgcttctt taatcgaatt agctttctca cgagctatat ccatagtccc tgaggctaaa
    15241 tgtgaaccaa aagagcctgc aaatttggca gcacttccca ttgcctcagc taagccaccg
    15301 gttttggagc ttttatccat cccagacagt gcattggtat cacctgagtt ggttgattga
    15361 ctggctgctt taaatgcagc attaagtgca gataatccac caccgctttg agcaagtacg
    15421 ctacctgcgg cacctgccat ggctgctcct gcaattccag cagctcccat tgcaccacca
    15481 agaccgagtc caccgccacc accagaaccg cctcctgaca caatggaggc gagtgcaggt
    15541 ggaattttat ctataagaac cagcaacagc acggcaacaa ctagcatgac aaacatctct
    15601 ttgagcatga tgttttcaga catcgctgcg taatattgat ctacaaagga tttaccgata
    15661 ccaataatca aaatcatggc aaaagcttgt aatgcgatac ctaatacggt tttgtagtag
    15721 ttaatcgcaa tatcctgtgt ccaacggcca ccgccaaacc ctaataaaac aatgccacca
    15781 taggttaaga tccaagcggt tattaagata atgagcatgt taatactgac taaagcaaga
    15841 ataatcagaa ttacccctgc aacgagcaat cctactgtag ttgctgcggg ggaccagata
    15901 gatgagctgt ctatagcttt tgagacaata tcaaacccaa catctacaat atcagaaggg
    15961 gagacatgtt cattaattcc agaagccttg gctgcaagtt gccgcatgga gtctatgatt
    16021 gcttttgcaa tggccggtcc attgtctaaa atccaataga aaaaaccgac aactacaaaa
    16081 aaccgtaccg tttcagctaa gaattcttga atgtctgctt tacgcagtgc catcaagccg
    16141 tatgtccaca ccatactaat tgaggctaaa gaccaaaaaa gatatctggc gtaactcaac
    16201 atagttttgc tccatagtgc ggatgtgcta gcaaaacgct gtaaaatatt atccagtaaa
    16261 tcacgactat ccatgccatg ccctttggca tggcagcaag cagaaaaccc aaataaacca
    16321 aacgcaatga cataggggat gattttttta ttcatagcat taccacttct taccagagct
    16381 tttggaataa ctaccccctc ggaaacacgc atcaccaata gcttgcagtt cttctgtagt
    16441 tcgaccctct ctggggctgc tacaggtgag tgttgccaag tgctcagctt ttttctgagc
    16501 cttggattca gcactgtcgc aacctgacaa cacacaaaca acaaccataa ttgataaaaa
    16561 taagatcttc attttcctct cctttttatg atggattacc acttttttcc tgaactttta
    16621 tggtatgcgc ctgatctgaa atgctcatcc cctgcggtct gtatcgcttc tttgttggca
    16681 ctcgctgcat ctttgaccgc ttgagcattc tgctgcgcta ataacacgcc tcggatttga
    16741 agcaattgat gagattgatt actggctaat tgagaagctg cttgaagagc ctgtttttgg
    16801 cctgcagcac tttgtgcctg agattgcaaa gtacgcaact ttgcagaatc attttttaag
    16861 ctctgttgtt gtttatcaat ccctttaagc atagcgtcat tggatcgctt ttcagcaaca
    16921 gaagctgcta atctgctttg tttgattttt ctaagttctt cagcagaaca ttgacctgtg
    16981 ccattaaaac acggggtctt ttggtagtat tcctgacttt ggaatcggtc caagtatcct
    17041 tggaggctgc cagcttcctg cttgtagtaa tcaatggtat cagtcgattc aagcaaatta
    17101 ttaataactg agttggcatt atcccattca aaggaagtca gtgacttggt gttgtctaac
    17161 atgttttggt attgatccat ctgattcttg tattgatcga cttgcgtttt atattcacta
    17221 agctgggttg caatcatttt tccattttga atccaattcg caatatcaaa cacaggcgcg
    17281 ccacccgcga atactggtaa aacaaagcaa taaataatca tggatttaaa tttcataatg
    17341 ctctccttca ttaataagca aattgcttat agggctgttt aaaggttaag tctttcacca
    17401 cgatgatatt gaaacgataa cctgggcgaa catttatggt gggggacaca ttcaggtttt
    17461 ttgaaaccag ttgcgaagtg acctcgccca actgttgtcc taaagcctga ctcatgacgc
    17521 tgccagctgt gggctgggca taaccgaatt ggctagattg attggtattt tggctataag
    17581 taataccagc aataatgcct gacattaaaa gagctgaacc ataaattcgt ccataatgat
    17641 gatctacctg atcacgaaat ccggcatatc ctgcactatc agcaccaggc atagagccaa
    17701 tatccagggc tttgctgtct ggaaaaacca agcgttgcca ggcaactaat acagagtttt
    17761 ggccaaaagg gacatcactg gaataaagcc ctaatatctt ggtaccttgg ggtattaaaa
    17821 gatatttccc ggtagcggtg tcatatacgt tttgtgatac ttgggcaata atttgacctg
    17881 gtaattcaga ggaaatgcca ctaatcatta cacctgggat cacgctgcca gctcttagtt
    17941 cgtatcgact gtttgggctt tgaagtgttg aatttaaatg ccagcgcatt tcattttctt
    18001 caccacccaa ggtttgtggc atgggtttgg cttgccttcc ttgtaataat gcgagctgct
    18061 ctttaaacgc caatgaaggg tcgatgttta ttgccatctc attgctattc atggacgttt
    18121 tggcagtgtc gcgattattc aatcgtgggt tatctaccat tacggaagtc ttagctttta
    18181 cagcctcttc aaattcttga gttttttcct gacgaatgcg ctcgatttca gcttcagata
    18241 catcttgtgt gagaacatct tctttaggta gaagttgttc aggaataggc aaaggagctg
    18301 cttcattgtt agcaatcgtg acactctggg gcggaattac tgctgattta tggtttccta
    18361 ccacgtcatt ggcaaggctc atggtatttt tctttgggtt tttaatcttc actatttctg
    18421 gtggctggtt ttgcgcatta gctcgtttat tggcaaccaa tgcgatgagc accacaaaaa
    18481 gagttaaaat accaattgca atcatgaggg gtacattatt cactcgtttt acacctgcgg
    18541 tttgaagctt ttgcggtgaa ttgtctggcg acaaataatc attattttta ttcatagttc
    18601 ttattcctta tgagtccatg tgctgatagg gatgatgttt tcatggcttt ttgcatacag
    18661 acggctcaat gtttgcattc ccacaaaaag acttactcga taactttgag gtttaagctc
    18721 atcaagcacg tagaaaaaat tagctttatt cttagggcat tgattttcat tgatgccata
    18781 accctttttt ctcaagtttt gaataagact taagccaaag ccatcgttga ctgcctgacc
    18841 aatacaaaat gtattttgag ccggtggata tactcgggtt aattgagtgg ctgcatctgc
    18901 cgccaaataa gcatctttgc cttgagacat ttgagtgaaa ttgccataac gcatgctggc
    18961 acagcttgat aacaagacag cagccaataa aacacttaat tttttcatct tcagcacctt
    19021 gtaatcgtaa ttttttcttg agaagaaccg ctgccgataa ctaagatggc tttatcaaaa
    19081 actccatcta cgatgtaacg acaaccctga agacgataat tgaccatgac ggactccgat
    19141 tttttaaata tccctcgact tcttaaaacc agcagcgcag gcgcttcact ttgagccatg
    19201 gttcttggca tctcaataat tgttttgaca ccattgttat aaactctaac tggcttaaat
    19261 cgagcattgc cttgaattcg ataattgaag ttaagatcgc ctagatattc attggtttct
    19321 ggtaaggtat ctacttttct tctttcagcc tgaatgcgtt ctagtaactg ccattttgct
    19381 tttgcttcat caggataact aaatgaaatg taaggcataa attcagttct atctgacttt
    19441 aatctaaagt gatatgttct tctgtctgtg gtgattacca aagaagaatc aagccctaca
    19501 tctttaggtt ttattagcag atggatttgt tgcatagctc cagatcctgt gagagatggt
    19561 tcaacaacaa agcgtgggtc accgacgttc atgtcgttaa actgctcacc tggttgaagg
    19621 gcaatatcac acacttgtaa aggggcacaa actaccctga tttgccctga accataaata
    19681 aaactgactg agccatcagc agaagcaaag ggtatacttg tcttttctcc ttgttgtgta
    19741 cgctctgcaa tatctaaacc ttgtttttcc tgactcgata gcttgggtac agtatccgaa
    19801 aaataatgct cggccagaat agtgttatcc aatgcaaaac ttgtgagcgg tgttaataaa
    19861 caaaatagct gcattactct tttcattgct gttctccatg ctttaattcg taggaccagt
    19921 taaaatcgcg cacataaatc aggtggggat ttttcaaaat ggactcactg gtggcatcat
    19981 tgagttcatt ctcctgatac atcgtaatca tcgctttcat catgcgaggc ttttctttaa
    20041 gtgagccatc acgatttctc actgtttcaa tccagtcaac ttgccaggtc tcatctgact
    20101 cttgaagtac tgaacgaatg tctatgctca caatctcaaa agctgctctt tcaaagggat
    20161 tagattgttg tttgtcgctg tagaattctt gaaccttggc aagggctgca tcattggcat
    20221 ttaaatagga atacacttga aacactgcct tcttttgtag ttccgcatca aggcttacca
    20281 tgcgaacgtt ttcaatgaaa tgcgctgctg cagcacgata atcatcaatg cttgcctcac
    20341 caaccttatc agcacgggta accgataagg tattgccatt ggcatcttgt tgaaacacca
    20401 aagggataaa tttggattgg ctacctatcg cgatgagtcc agcaactgcc gccaatgcga
    20461 ttaataggct acctaatccc accaattgcc aaacttgtag tgatttcata agtcctgcgg
    20521 tatgaacatt ccatgcccgt ctggcattaa gataaggatt attcgttaca ctttttttca
    20581 tgcttcttct ccgtattgtt ccattggaat acctttttct gaaagccatt gaccaaccca
    20641 ttcctcccca tgcttcaact ccagttgctt cattcgtgca atagaatcgg gatcggtagc
    20701 acccacaaac gccaaggcat aaggtccaag cgctaattga taaagacgct ggcctttttc
    20761 actgacatag tagtaatcac gcttgggttg ggctgctgcg atgatgtcaa tctgacgcgg
    20821 atttaagccc atacgctcat aaatggttcg tgtttcagga tctcttgcat aaagattggg
    20881 taaaaagatt ttggtagcgg ttgattcgac aataatgtct aaaatgccag aattggctgc
    20941 atgcgacaga tgttgggttg ccataaacac aacacagttt ttcttagcca ttgaatcaag
    21001 ccattcagca attttgtttt taaacacagg atgtgccagc attaaccagg cttcatcaag
    21061 caagattagg gtggggcgac catctaatga ggtttcaata cgcctaaaga gataaagcaa
    21121 cacaggcaat gcaaattttt cacctaagcc cattaaatgc tcgatttcaa aggtcataaa
    21181 ggaagacaaa ccaagcccat cactttctgc atccaataaa tgccccatta acccatctat
    21241 ggtgtactgc tttaacgttt ctctaatggg ctcatcttga atcgtcatga caaattcaga
    21301 taaggtttta gaaccacttt ggtgcatgct tagaatggca tggccgattt cattgcgctg
    21361 agctggtgtg gtattcaaac cattcaaagc tagaatcgta tcaatccact ccatagccca
    21421 agctctatcg gctttagttt ctaaaaactg caatggcgca aaggcaaggc gtgagtcttt
    21481 atctgcgatg gtgaaatgct ccccacctgt tgccttgcaa gttggataca tggacatgcc
    21541 tttatcaaaa gaataaatgc gggcatcttt atatcgacgc caggacaatg ccaataaacc
    21601 aagatgggtt gatttaccgg agcgtgtggg accaaacata atgccatggc ctaagtctct
    21661 gacatgaaga ttcaaacgaa atggagcatt accgctggtg acacagtgca gcaaaggcgg
    21721 tgattgaggg ggaaatagtg ggcaaggcgc tttattttca ccagtccaaa tgctggatgt
    21781 tggtaacaaa tcagccagat tcatggtatt cattagaggc cttctgatgt tttcaacacc
    21841 atgaccaggt aggctaccca tataggcatc catggtatta atggtttctg tacgtgcgct
    21901 aaaccctaat gcattgatat tcttttcgat gaatagggct gctttttcaa cgttcgctct
    21961 atcttcgtcc attaacacca caaccgatgt atagtaacct tggcctacca ttccggaatt
    22021 ggtttcggca atagctgctt gcgcatcatt aaccatgccg agcgcgtctt catcaacgac
    22081 acccgagttg gtattaaaca tttggtcaaa aaagcctctg accttttgtt tccatttttt
    22141 acggaattta gtgaaatgag ctacagcctc atggggatcc atgaatatga agcgagagtt
    22201 ccagcgatat tccacaggca gttgagtcaa cagtgttaaa atacctgggt aagactccat
    22261 ggggaatcct tcaatagcaa cacactgaat gaactttcgg ccaattttag gtgtaattcc
    22321 tggagttaat tcttgaccgc ctattaaagc atccagataa attggatttt taggcagatt
    22381 cacagggtga ttaatccctg tgacacaata ttgaatgtgt cttaaaaagt tatcttgagt
    22441 gactttattg ttttcattga tgaatagttc tgagcctagc ctttcaaggt gaagtgccgc
    22501 actcatacgt gactcaaagt tcctgcatga ttgcttgaag gtttcaatta attgatgggt
    22561 tttggctttt tggtttaact tttcccctga gtcatcaaac atcaattcca caaaacgctt
    22621 ttgtgccaat accggtggat accaggttag tgttataaca aaataacctt catacaatgc
    22681 ccctatactt tcaaagtaca gtcttctttc ttcatcaaca gcttttgata ccggatccgg
    22741 gaagtatgaa gcactctgct cactgtaacc tggagcagca cgtcttatgg catcgacatg
    22801 aatcatccag ccattaccca atgaggacat tgcctgattg attcgaaaag ccatcatttc
    22861 acgggcttca tctgtagtgt tggctatgtc ttcaccttga tacaaccagg ctgccataaa
    22921 agagccattt ttacctacaa tcacaccatc atcgactact gctgcataat tcagtaaatc
    22981 aactagtccc gctgtatgac ttcgatgtct tctaacatgg ttcttacggc ttttcattcg
    23041 agctgcgcta aacaatgtac ccaacaacaa aagcccagta atactgatta aaaaagtaat
    23101 gaatccaatc ataggtaacc ccctttatta ttacgaaaag gggtagaacg tgctggataa
    23161 taagcctgat agcctctttg tctcagatag actgctctca tataaggatc agcttttgcc
    23221 atgagcctta gaccttttaa ggataaaaac caaagggcaa agccaaaaat agcggcaagc
    23281 caatcttgag ctgcaaatac taagattgct gagagtaaac ctgaaaacat aaccagctcg
    23341 cggtcaccac ccatgaaaag gcttgggcgg ttaccacatc tgcgtatagg aatggtgcgt
    23401 aggctcatgg ctgcaactcc gtagcactcg ttaaaactga gggtttagca atttcagctc
    23461 ccttgcctgt aatcgcggtc atggtatttt gtgcggcaac caggaatgaa agcactaata
    23521 caataaagag aagggttcgc aaaaaaccat tcatttcacc accaaaaatc agcgtggctc
    23581 ctgcaccaac taaaccaata attgcagcag taaaggcaaa tgggccggta atggattttt
    23641 gtattttggt taaataggag tcaaaaggta aacctccacc ggcactagaa gcacatgcag
    23701 gatctgttat aaacagaagc aataaaacaa tcgctcctat catccataga cttcgataat
    23761 ttatattgat tgcttggttc attcatattc tccttaaata agttgttcaa tgttgtaatg
    23821 accattttca tatcctttaa ccgatatgat ttcttcaact ctgcgtcctt gcggggttct
    23881 tgcgatatgc accacacaat gaactgtctc accaatcaaa ggttcaattt cggtaggcgc
    23941 tgcggggtta cgggtaataa gtgatttcaa acgatggagt cctgcgaggc agttattagc
    24001 atgcaaagtg gcagcaccgc cctcatgacc tgtattccag gcatcaagta aatccagcgc
    24061 ttcactgcct ctaacttcac caaccaggat tcggtctggc ctcatcctaa gcgtggtttt
    24121 caaaagctgg gtcatattga catcaatggt ggtgtgatat tggacgcagt tctctgcagc
    24181 acattgaatt tcaccagtat cttcaatgat gaagacacgc tcggttggtg agctacaaac
    24241 catttcattg ataatggcgt ttactaaagt agttttacct gaacctgtgc cgccaatgac
    24301 gagaatgttt cgatgtgcaa taacagcctt cttaatcacc gcacattgtg cttctgtcat
    24361 aattcctgat tttacatagt catctagtgt aaaaactgaa atggcttttt tacgaatcgc
    24421 aaatgttggt tttgatacca ctggtggcag ctgccctgca aaacgtgaac catccaatgg
    24481 cagctcacct tccaatattg gattaaaacg tgttatctcc ttaccatgaa agccggcaat
    24541 agttttaatg atggattcag cacgcgcgtc attgatacta ccgatacaac gcatggtttc
    24601 accaaggcgt tcctgccaaa gcttgccatc tgcattgagc atgatttcaa cggttttagg
    24661 atcacttaac gcttgttcaa tcatgccacc caagtcacgc cttagctttt cacgggcgcg
    24721 gtctttaact gtgatccctt gttcttcttg tgcattcata cgatataatc cttgattact
    24781 gatgccttta agctgacttc aatccgtttt tgaatttgct aattaaacga tcaacaatgc
    24841 tttccttatt ggagggaaag tcgctgaacc attgatcttt ttgacgttca tgataaatgc
    24901 gtctttgaat ttcatcacgg tgaactggga ttgattgagg ggcatcgaaa gcaaggcgaa
    24961 cattaccatc tcgataaccc accacagtgc agtaaacttc ctcactgata acaactgatt
    25021 caccaacttt tcttgttaaa accaacataa tgcgtctcct taaagttatt taaataaata
    25081 ggccttgcac tgcctgatat tctcttcaac cacttctttg ctggctggtt gatatttttc
    25141 tggtctgaca ttaaaagcat ttcgcctttt gatatcacga caaaagccaa tcatttgcgg
    25201 cagggtaggt ggcatttcgc aatgatctcg gcaatcaatg acggcctgat tcaaaatctc
    25261 atcactgaac tgacttaacc cctccaacca ctccttcttg gcaaactcca aaaaaccatc
    25321 attcttgaac tgactgcgcc acaaatgccc gtagtaaact gcaaagcggg taaacaggtt
    25381 ttcaatacat ttttttctac tgtcggtagc attgcaggtt gatgacgttt gactgcttgt
    25441 attcgtcttc atcggtgctt gctgtctctc ctgtttctgg gttccagaat ggatcaatgg
    25501 ctccgctctt agtatttgtg ccgcgcttct cattcctttt ctcctgcgtt aattcgattt
    25561 tgacttcatc ttcccaacat ttttgagata gccagttagc tggaaatttc catgctggaa
    25621 cccattctcc atgtgcttct tttgcttcac gcgctgtgat ttgttgataa agtgcttgca
    25681 atatgcattg cagcaaaggt tcgtctggat tgatttgtgt aaagatttca aaggcacgtt
    25741 cacgagactt tttctcgggg tacatacgcc aaaaatggtc gaagcgttgt aataaataaa
    25801 tataattatt ttcttttatt agaggtgtgt cggcttttat gatttcattt gtgtcggctt
    25861 tttcacatac acgctcggaa taccctgtat ttacaggggg ggttgcatgc ggctttgtgt
    25921 cggcttgctg tgtcgggttt gtgccgggtt tattttggac acaataactg atatccgcta
    25981 aaaggcattt taaaataaga tgtttatcta aagactgaat gacaataagt cctgctcgtt
    26041 caagcccttt aatgacccga cgaatttgct gacggctagg acttcctgac tcagtaatac
    26101 cttgatgagg ttcaatataa agtgtctcag ccaaggattg gtaactaatc cgtcgcttaa
    26161 ttcctactat gaaagtattg cgatccatat agggacgaat acctcttaag taggtaattt
    26221 gctggatata tgaaagtcca cagagcgcat ccaattcatt gtcattcact gtaaagtaca
    26281 taaatagtcc gttattcctt aactaaaagt cttagcgctc aatgaaactt gtgataacat
    26341 tttgagaact taagacaaaa aatagctctt aatgagctta actactcgaa acgagtagtt
    26401 attgaatctc aaaataacac atattgagct atagtcaata tgtaaatgga ttaatttatt
    26461 aaccaaaaac cgtggaatag gtatgaacat caaagagaaa atcggccaaa gaatcatgaa
    26521 tgaacgaaag gccaaaggat tgacgagaaa agcattggct gagttgacca gtgagttgaa
    26581 aatttcacgt attaataatt atgaaagagg ggaccgtacc ccgggcccaa cagaaattaa
    26641 attgctggct gacgcgctgg aagtttcagc ctcttattta atgtgtttaa ccgataaccg
    26701 tgaagggaag atgacaaaat cccttggcat gggggcattg attcctgttt tggattacaa
    26761 acaagcaatt gattcagcta ctttcatcca gaaaatcaaa gaggatgttg atactaaagt
    26821 tgagtttatt cctgtcagta cagctgtcag tgatagcatt ggaaaaaatg ctttcgctct
    26881 ccagattaag gatgaaagca tgatgccaga gtttcggatt agtgatgtac ttatcgtaga
    26941 tcctgataca agccctaaac ctggcgattt tatagtggca ttgattgaag gagatcagga
    27001 agtcattgtt cgcaagtata agcaattatc ggcctctaaa gaggctcacc aatttgaatt
    27061 aattgcactt aacgaggatt gggctgacat tcgagtgggt tccagcgaaa tgcaagccca
    27121 gattattggg tgtggtgtta gcttggttcg aggattgaaa aactaaatac ttaggaatct
    27181 cacaccgcaa tccagatagt cattgccatg acatgttaag agtattattt gatgtttttg
    27241 taggatttgt ttcattttct cgcatctgag atcatcagaa ttaaccaatg acatgttttt
    27301 ctccgttaat gttgaacaca acaaaaaaac ttagtttgct ttatgaaagt tcaattgaag
    27361 cggtgatcca tttagtttgc taaattaatg gttccattac agcgattatt tggtggtccc
    27421 tttgaaggtg aatgacaatt gctatcaaaa tagttttgct ataattaaca acataatcaa
    27481 agcatgcaag gaagagtcat catgccattt gatccaacta ttctaactga atcaaatcta
    27541 aataggtatg ataagcaact ggaaagaatt tccaatgact ctggttatac ggaaaatatc
    27601 caagaaacag ttgttaatgc aattgataac attaccactg gttgcaagtc atttgtgatt
    27661 tatggagagc cacaaagcgg aaaaactgag atgatgatcg ctttaacggc aaaattactt
    27721 gatgaagaaa ataagataat cattatacta ctaaatgaca atgtacaact actaaatcaa
    27781 aatttggata ggtttaaaag atcaggtatc aatccgacgc caagaacctt cgatgaagtt
    27841 attaatcaag ttacagatat tagaaatagc gaatggatta tcttctccaa aaaaaactcc
    27901 aaagatttac agaagcttct tacaattgtt ggttccgaaa aaaataaagt catcatcgat
    27961 gatgaggcag attatgccag cccaaatgct aaaattaaca agcaagaaca aacaaaaata
    28021 aacgaattag ttgaaaaact tataagtgat aatggtattt acattggtgt tacagcaact
    28081 ccagcaaggc tagatttaaa taatacattt cgcaatgaaa atcaaaaatg ggtctacttt
    28141 catccccacg aaaattattg tggacatgat atcttctttt ctccggcaga gccgattggc
    28201 aatcttatta aattaatacc agacagcgag gatagcaaaa aatctttaag tacttgtcta
    28261 tttagttttc ttataaatat tgcatactta aacctacgaa agaatagtaa tgaggaaaat
    28321 tattgtatgc ttgttcatac aagcggaaaa aaaatagacc atatagaaga tcaaaaaaat
    28381 cttgaaaaga tattcaatat cttaactcat cagagtgatt ctaaatttac gaaatactat
    28441 gaaaaaatat gggaaataac taataataag tttaattgtg acgaagagtt aacccaggaa
    28501 ataactaaat atattcgaaa caatatcaaa cgttaccaaa taattgtaat aaatagcgaa
    28561 catgacaaag agaaagtaaa aggagctaca agaccatcat cattatttac aattgcaatt
    28621 ggcgggaata ttgtatcaag aggagttact ttcgaaaatt taatttccat gtactttaca
    28681 agaacaacta aacattacat tcagcaagac acttacattc aacgtgcaag aatgtttgga
    28741 tatagaaaag gatatttagg acaatttgaa ttacatattc ccgaacagct atacaaagaa
    28801 tggcaaaaat gctttgttta tcacaggtta tccttagagt taattaagac aggtcgaggt
    28861 gttcctagct ggtttgaaga taaaagagtt acatccgtag ctaaacctag catagacaaa
    28921 aatacggtct tgttgtatca cggtgaaatg gcatttgcga ttttcaaata cactaatgaa
    28981 atagaaagca tcatcaataa agaaagtcat ttttattttg aaaaattaac aaaactgcac
    29041 accattatag gtgaaaagtc acttcctatg tgtttaatag actttataca tcgaacatca
    29101 aacggtcata atgataaaat tgctatccat aaccctggtt ctgtttcaaa cagacgaacg
    29161 gttgatccta ttacgataac gcgaactggt ggaggtatgc tgggtactgg tgacttagag
    29221 ttaaacaagt ttccacatgc atatcatcat attaagattt tctatactga aaatggcgac
    29281 gctaaacttt attataaata tcagcgtgaa cggataaaga tacgtagaaa tgtaggtaat
//
LOCUS       DATNSN010000025        27913 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.025, whole genome shotgun sequence.
ACCESSION   DATNSN010000025 DATNSN010000000
VERSION     DATNSN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27913
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.025"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(317..1375)
                     /locus_tag="VAH76_000896"
     CDS             complement(317..1375)
                     /locus_tag="VAH76_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEO1385543.1"
                     /translation="MTEEVLFSQEGQLGFITLNRPKALNALTLTMIMALQKQLSIWKE
                     DNSIKAVVVQAVPGNAFCAGGDIRWLYNAGRSKDSEQMQFFWHEYRLNHFIHHFGKPY
                     ISLLDGITMGGGVGISLHGSHPVASERFVFAMPETGIGFFPDIGASYLLNKCPGFLGV
                     YLGLTGNKLGPHDARKAGLVKQIVFAEQMQSMIEALKREDLSEDAFNRVDQCISSFAS
                     DAMPTEASQIKPLIDVCFSKPSVELIRESLQSTDGVWALGVDNTLLQKSPLSLKITLA
                     QIQKAKGLSLAECLKMDFDLASHFMKGSDFYEGVRSLLINKDKNPQWRPSSLELVTDA
                     MVVSYFESSSSGLELMIL"
     gene            complement(1378..2154)
                     /locus_tag="VAH76_000897"
     CDS             complement(1378..2154)
                     /locus_tag="VAH76_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEO1385544.1"
                     /translation="MNLIEQDIDNNGILTLTLNRPEKLNALSTDVLSALNELFFQAKS
                     NPKVKALLLTGNGKAFCAGADINRLAECNAQTGYEFACQGQEVFRLLETMGKPSLAAV
                     NGFAFGGGCELAISATLRIASNKAMFGQPEVKLGVIPGYGGTQRLARLIGKGRAMDLC
                     LTGRFINAETALNWGLVSEVVAPEDLLTQGKNILNGILSMAPLAVASVMEVIDHGYDL
                     SLTEALHLEAIHFAKVCATEDKKEGVAAFLDKRTADFKGV"
     gene            complement(2171..3325)
                     /locus_tag="VAH76_000898"
     CDS             complement(2171..3325)
                     /locus_tag="VAH76_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEO1385545.1"
                     /translation="MHFNFTDEHLAFREMASEFARNKLAPMADFWDEQGYFPIEILRE
                     AAQLGMAGMVVREDIGGAQLSRLDAALIFEQLATGCIPTSAYLSIHNMVASLVDRYGS
                     QEIRKKWGPKLTSMEVIASYCLTEPESGSDAASLKTRAVKEGEYYVLNGAKAFISGGS
                     VSDVYLCMVRTGDESHHGISCLLIEKNTPGLTFGKLEKKLGWRNQPTCMLYFENCRVP
                     IANRVGEEGMGFKIALNALNGGRVNIASCSLGGALACLRMSQSYMHERKQFGKPLTQM
                     QALRFYFADMLTDYEAARLMVYRAAAAMDNDDPNAPMYCAMGKRLATDVAFRISDKAM
                     QLHGGYGYLRDYQIERIFRDLRVHQILEGTNEIMREIIAKASLDEEYFIE"
     gene            3439..5265
                     /gene="recQ"
                     /locus_tag="VAH76_000899"
     CDS             3439..5265
                     /gene="recQ"
                     /locus_tag="VAH76_000899"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEO1385546.1"
                     /translation="MGTLAQPSFINPNALAVLKKYFGFDSFRPPQEKIIEDVISGEDV
                     LVLMPTGGGKSLCYQIPAIVRRGVGIVVSPLIALMEDQVTALKLQGIRAAYYNSSLTA
                     EEARNVLNQLHHNELDLLYIAPERLISTSFLDRLSECTISLFAIDEAHCISQWGHDFR
                     PEYACLGLLKTNFPDIPMIALTATADKQTREDIVTKLNYQPKKYVVSFNRPNIHYKVV
                     PKTNAVKQLNLFLQSDTQQSGIIYCGTRHTVEHLTAKLQELGLKARAYHAGLSHTERR
                     EVQNLFRYDRIDIVVATIAFGMGIDKPNVRFVVHHDLPKSIEGYYQETGRAGRDGLPA
                     KALLLYDAADSARLRSWIMNIPLDEQRHIETNKLNHMLAFAEASHCRRQILLRYFDEP
                     SHTECKNCDVCDNPPQTADATEDAQKFLSCIYRLKQSYGLTHTIDVLRGSLSEKIKQS
                     GHEHLSTFGIGKEKSANYWKHLAWQLIHKDYCLQDMNHFNVLRLTPKAIPLLKGEESI
                     SLTIPNNDLNSSKKKGKQSPSFKPESNPLFEKLRALRRQLADEENKPPFMIFSDATLH
                     EMAQLKPKNSKQLLNVSGVGQHKLARYGHYFLEALNEFSEVE"
     gene            5275..5826
                     /locus_tag="VAH76_000900"
     CDS             5275..5826
                     /locus_tag="VAH76_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEO1385547.1"
                     /translation="MRKLPRPFYERDTILVAKELLGKYLVHHDGLEEKIGRIVEVEAY
                     LGQHDLACHSSKGLTKRTKVMFGPAGYAYVYLIYGMYYCMNVVTEKEGIGSAVLIRAL
                     EPIKNIQDRTQGPGLLSKAMRIDSKLNHRDLLSNDFYIAEPNSPTDFTIIEKPRVGVH
                     YAKEWANELLRFYIKDNPYISKT"
     gene            complement(6021..6425)
                     /locus_tag="VAH76_000901"
     CDS             complement(6021..6425)
                     /locus_tag="VAH76_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEO1385548.1"
                     /translation="MKKLLSASVITLMITGCAAIPADPQAERVIASPNPAPKGCKYVG
                     QVVGNQGNFFTGGFTSNRNLEEGAMNDLKNKASKKGANYIQLVTNRAGVTGSMSGSFD
                     RHGGFMSGGSEQTNVTNLGNAYICPPKAIGLE"
     gene            complement(6481..6684)
                     /locus_tag="VAH76_000902"
     CDS             complement(6481..6684)
                     /locus_tag="VAH76_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEO1385549.1"
                     /translation="MTDKVYQIVELIGTSQEGIEKAIDNAIKQVAKTHGKLDWFEVVE
                     TRGFIDDNKVKYYQVHLKVGCNV"
     gene            complement(6674..7360)
                     /locus_tag="VAH76_000903"
     CDS             complement(6674..7360)
                     /locus_tag="VAH76_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO1385550.1"
                     /translation="MNEWWLIILLVVLAVFACTLIIYPLRRNRLVSLLLAPIIFIMVF
                     TGYYFWGDFARWQEYLHRNEAQELAQSMLKSIKSPQELIDKLKAKLDAQPESAKGWYL
                     LGRLYSSQKDNQNASLAFAKAHQLKPEDEQFAVNYAHSLWQLSNQQFNPDILEIFHTL
                     LKNNPRQPDALAMLAMHAYLSHDYEAAINYWQRLLQLAPAQSEEALAIRKAIAKAEKQ
                     INRGGKKSDR"
     gene            complement(7353..7754)
                     /locus_tag="VAH76_000904"
     CDS             complement(7353..7754)
                     /locus_tag="VAH76_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEO1385551.1"
                     /translation="MRVFHFCFKVILLLLLVSVAFASSMYPLDSARKEAQFNHLLKDL
                     RCLVCQNQDLADSNAQLAKDLRFQVYELVKEGKSDSEIMDYLTARYGDFILFKPPVKS
                     VTLLLWFGPFLFLLIGFIIFWRTCFKRNSHE"
     gene            complement(7754..8287)
                     /locus_tag="VAH76_000905"
     CDS             complement(7754..8287)
                     /locus_tag="VAH76_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEO1385552.1"
                     /translation="MKKIGWRVIPIAIFCLLCFFLWRGLSLDPHHLPSARIGKPLPDF
                     ALPELQNPDSTFSAMNFHNQVVLLNVWASWCDACVEEQVFMLQLAREGVPIYGLNYKD
                     NPEHARAWLAQWGNPYRLIGQDSEGKVAIDLGVYGAPETFVIDKKGIVRYRHVGIMNQ
                     KTWLTDILPLMRNLEKS"
     gene            complement(8284..10236)
                     /locus_tag="VAH76_000906"
     CDS             complement(8284..10236)
                     /locus_tag="VAH76_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEO1385553.1"
                     /translation="MIAELGLFFLILALVASLMLALIPLAGLQFNRKDWIDSSRNYVV
                     LQFLFVACAYLCLTACFLMDDFSVIYVLNNSSLLLPWFYKFCAVWGGHEGSMLLWVAI
                     LSFWMILVCFFSSSLDTEMRARVLVILGWLSIGFILFLLMTSNPFLRQFQVLNTQGRD
                     LNPLLQDPGFLFHPPMLYMGYVGFCVAFAFAIAALWAGKVETSWSKWTRPWTLAAWCC
                     LTAGITLGSWWAYRELGWGGWWFWDPVENASFMPWLVGTALLHSLAVTEQRQQFKAWT
                     MLLAIAAFSLSLIGTFLVRSGVLTSVHAFAVDPQRGLYILGFLLFVIGGSLTLFLFRA
                     QTLQVNKHPGLLSRESALLLNNVFLVVIMLTVLMGTVYPLLVEGLGLGKLSVGAPYFN
                     AVFVPLIIPLLLLMGLGVRLKWQNDCWRDVFRKLNRIFLLSLILPVILLFCTSTEIHL
                     TALLGLILAFWVLLSTANAAYHKVRDRGVAGVGQAYWGMVLAHCGVAATVIGIAVSSA
                     YGVQDDVRMSPGKQVSLLDYQIGFINQTPLNGPNYKGTRAEFSIQYQNKTRQIFPEKR
                     VYTIGQMPMTESAIDVTPFRDIYIALGEPLSEDAWSVRIYYKPLVRWIWAGGFMILAG
                     GVLALTDRRYYRKRQVTVMAGQEIKI"
     gene            complement(10233..10664)
                     /gene="ccmE"
                     /locus_tag="VAH76_000907"
     CDS             complement(10233..10664)
                     /gene="ccmE"
                     /locus_tag="VAH76_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEO1385554.1"
                     /translation="MTPVRRRKLFILLFALSVLSAAAALVLYALRQNISLFYTPTQIV
                     AGEAPAKHHIRVGGMVEANSIVRAKKGLDVQFKITDFENTIVVTYSGILPDLFREGQG
                     IVAEGEVTDNHHFHAIQVLAKHDANYMPPQVKSALADKVKQ"
     gene            complement(10670..10828)
                     /gene="ccmD"
                     /locus_tag="VAH76_000908"
     CDS             complement(10670..10828)
                     /gene="ccmD"
                     /locus_tag="VAH76_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEO1385555.1"
                     /translation="MNQFFEWLAMGGYSLYVWPAYGLVCVVLGMNILGIKWQRTRTRK
                     KLQRWFKR"
     gene            complement(10812..11567)
                     /gene="ccsA"
                     /locus_tag="VAH76_000909"
     CDS             complement(10812..11567)
                     /gene="ccsA"
                     /locus_tag="VAH76_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEO1385556.1"
                     /translation="MWKILYQLASPKNFYNYAGRLIPWLAVSALATIAIGMVWGLVFA
                     PPDYQQGDAYRIIFVHVPSAFLSMALYAWMGFLAILLLVWRIKMAGLLIHKVAQLGAC
                     MAFLALITGSIWGKPMWGAWWVWDARLTSELILLLLYLAILATYQAVKNKEDGDKIIA
                     ILALVGLIDLPIIHYSVYWWNTLHQGATLSVFAKPKIAPSMLYPLLITLLGFFLYSLW
                     IILEKARNEVLFRERKQSWVKVQFEEESDESVF"
     gene            complement(11815..13383)
                     /locus_tag="VAH76_000910"
     CDS             complement(11815..13383)
                     /locus_tag="VAH76_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISLpn13 family transposase"
                     /protein_id="HEO1385557.1"
                     /translation="MIKQENWIYPIELSKKEEKICSKLKQNGKLFIFLRKNRHLIFTA
                     DISQKLLSMYADYPRGKPLISPTLLAMVSILQAYEQLSDSSAVLETMFDQRWQMVLGC
                     LGAEESIFSQGTLCDFRHRLVKHNLDTMLLEQTVEVAKEVGGFCYKQLRIALDSAPLE
                     GAGRVEDTFNLIGHALELVVDCAAQIKQVDEIEIIKEAGTYLIGRSSIKAALDIDWSD
                     IQEKQKAINTLLKDVEALQRWLSGQSDSLINNQYLKEALSLLDTVLNQNIEPDPDGGS
                     KIIRGVSTDRRISISDSDMRHGRKSSSRTINGFKQHIAIDLDHKLILATSVRPANEPE
                     HEASQWLKPKVEKYGEVASKSIDRGYLSASWTAELFSQGKKIIAKPWNPATHGKYGKK
                     DFIIDLQNSMVICPAGKQQKITGKPGSLMAKFSAKLCGACPIKNKCSDSKTGRVIQIH
                     DQEKMLQALKYYVTSPEGRQEARERVKVEHALATICNRKGPRARYIGLRLNEYDLNRT
                     AMITNLHIAMNLAA"
     gene            complement(13469..14149)
                     /gene="ccmB"
                     /locus_tag="VAH76_000911"
     CDS             complement(13469..14149)
                     /gene="ccmB"
                     /locus_tag="VAH76_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEO1385558.1"
                     /translation="MNSPFQLFGKQFRREILIQVRQIRYLVNSCLFFLMLLFIFPLTL
                     RPELMLMRMFAPGLVWMAILLSMLLSLERLFQQDYEQGVLEQWLVSGQPLNLLVTAKV
                     MAHWLISVTPLLLLSPLIAILFSFNVWETCILLLSLICGTPAILFLCALAAAFGIGIN
                     QRGALMALILLPLTLPLLIFGSGTLSVAMQGLPVSGYLALLLAMSLLAVGFLPYAISA
                     VIRISHID"
     gene            complement(14146..14748)
                     /gene="ccmA"
                     /locus_tag="VAH76_000912"
     CDS             complement(14146..14748)
                     /gene="ccmA"
                     /locus_tag="VAH76_000912"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEO1385559.1"
                     /translation="MLDVIELDFDYHDQPLLQQISFHLPAGGLLHLKGSNGAGKTTLL
                     KLIAGLLNPEKGEILFERRSIKKDLCTYQKQLCFVGHRSGINPYLTLRENCLYDIHFS
                     PGAVGITELCRLFSLEHLIDYPCGLLSSGQKRQVALLRLWMSKAKLWLLDEPLVALDE
                     LSLLTIIRKIQEHRAKGGAVLLTSHQDLPLNKADYEEYHL"
     gene            14896..15831
                     /gene="sohB"
                     /locus_tag="VAH76_000913"
     CDS             14896..15831
                     /gene="sohB"
                     /locus_tag="VAH76_000913"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEO1385560.1"
                     /translation="MEFLSEYGMFLLKCITLVIALLILLAGIFSMGRKTKPKLEITSL
                     NEEYQHLNALMNKEILGKKPGKKKKDKTKRPVLYVIDFSGDIKATQVEQLRDEVTSVL
                     SIAKPEDEVLVRLESPGGAVNGYGLAASQLQRIRDKKIPLTVSIDKMAASGGYLMACV
                     ANKIIAAPFAIIGSIGVVAQIPNFHRWLKKNNIDVELLTAGEYKRTLTLFAENTEKGR
                     KKFQEDLEKIHTAFREYVLKNRSQLDIDKVATGEHWIAKDAFDLRLVDKLATSDEYLI
                     EKMAEFNAFKLTVHAKLPIISKVLKPAMRLIHPWI"
     gene            16054..16335
                     /locus_tag="VAH76_000914"
     CDS             16054..16335
                     /locus_tag="VAH76_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385561.1"
                     /translation="MSDFKSKLPDLNELTSMTAKLFKGIKTSVSEIIQDYKQKRAQPV
                     STEEKTTENKPAESKAEPKAESKTTENKTEPKTQSKPESNTEETQEKEK"
     gene            complement(16379..17794)
                     /locus_tag="VAH76_000915"
     CDS             complement(16379..17794)
                     /locus_tag="VAH76_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEO1385562.1"
                     /translation="MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVN
                     PNPSVIVLGACTSFEKMMDELDTLVKKFPKTPILVIDAVLSTSECADRNIVDCLSFPF
                     SYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
                     VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAI
                     TSRQGRFELANGGTLFLDEIGDMPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAA
                     THRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTV
                     RLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGENKPSCPEA
                     SGAERETLLQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMR
                     RTTLVEKMRKYGLTRPERMER"
     gene            complement(17990..18583)
                     /locus_tag="VAH76_000916"
     CDS             complement(17990..18583)
                     /locus_tag="VAH76_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385563.1"
                     /translation="MSNFRNFLVFILLFFSFCGQLTASSKQNLAFRNFWHPTYLGQRL
                     DYCTLDGKECGKEVANRYCQMLGYDYSTQNVIAYNVGLTNYLGSKAQCKGWRCNGFMT
                     IACAIGLSHTPPKPYHYREKKFAVPRYNDYRVDWCMERNKGCGKQAANSFCNRMGYMQ
                     AKDFVKQAQVSATKTIGSQELCFGNQCNAFKMIICYR"
     gene            18972..19988
                     /locus_tag="VAH76_000917"
     CDS             18972..19988
                     /locus_tag="VAH76_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385564.1"
                     /translation="MKKYLTFIVSGLALIAIIWFIRHLWIIETVKVVHPVYGTAVEAV
                     YATGTVEPTVMMPIASQNTARLIKLNADEGSEVVKGQILANLEDEHLQHNLKELKEHE
                     KLAKIRYERNAQLVKKGVISKDEYDRTLSEWQAASAAVRRIEAQISYLQLIAPADGRI
                     IKRDGEVGQLIPANQPVFWLSCCAPLRVSAEVDEEDIVLVRVGQKVLIRSDAFPNQIF
                     YGRVKSITPKGDPIARSYRVRISFSENNPLLIGMTAETNIIVHEKKNALLLPVSSISE
                     NKVWIVQDDGKLSQNTVTIGARGLKQAEILKGVNINDLVVLQPDNKLKNGQKVRFILV
                     KQEE"
     gene            19988..21238
                     /locus_tag="VAH76_000918"
     CDS             19988..21238
                     /locus_tag="VAH76_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385565.1"
                     /translation="MKLFLKIALKHLLARKRQSLVSLMGIVLGVAFFLTISSLMQGSE
                     KDFIKRLVDNSPHITIIDEYRNPRIQPIQQLHTHGAIEIRSVKPLTEPRGIRGYEQTI
                     NNLSLTIPGIVISPVLIGQALINYAGKDYSVTLNGMIPEEIKKVSTIENYMTSGSIDD
                     LIVNPDGIVLGSELLRKLSLTKDDNITVTAPNGQIRTFKILGVFRTGRSDFDTNQAFI
                     SIKRAQALLNRPNRSNNIIIKLPNPYQAYDIAAQIEKQIGYKTISWQEKSEDLLNTLV
                     IRNIIMYSVVSAVLIVAAFGIYNVISTVVMEKHRDIAILKSIGFQKHDIQFIFIIEGF
                     LLGLVGCLLGLPLGSGLMYSLMQVQFKPPGSSELINMPLDWSYLQFVIATAFAMSASI
                     IAAALPAHKAALVHPVDILRGGGM"
     gene            21235..21912
                     /locus_tag="VAH76_000919"
     CDS             21235..21912
                     /locus_tag="VAH76_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385566.1"
                     /translation="MNKIVIKTKELSRQLSGEVPVTLVKDINLEVREGEFLVISGPSG
                     SGKSSLLYLLGLLDRPSQGTIWLNNEDVSTYEEEKMAWLRLTQIGYVFQFHFLLPEFT
                     ALENVMLPMQRLGKLSLGEMKNRATKLLTSLGLEEQLSKLPKQLSGGQSQRVAIARAL
                     ANDPILLLADEPTGNLDTVSSTNVQNILKELAHEYRRSVIIVTHDMNFAATADRVIHI
                     VDGKMQE"
     gene            complement(22098..22856)
                     /locus_tag="VAH76_000920"
     CDS             complement(22098..22856)
                     /locus_tag="VAH76_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO1385567.1"
                     /translation="MITRDELSLFLLDFLNCSQYQDYAPNGIQVEGKDKIKRICTAVS
                     ASEDVISQAIEQQADALLVHHGYFWKGENPVISGMKRRRIAKLLGHNMNLFAYHLPLD
                     CHPELGNNASLANLLLIESHEMHKVNNTANLLWSGKLSKAMNSEQFSSFLEHKLGRYP
                     VHIAGNEKMIHSIAWCTGAAQDFIEEAYQLGVDAYLSGEVSERTFYQAMELGIQYFSC
                     GHHATERFGIQSLGVYLANYFDLEHWFIDSPNPI"
     gene            complement(22853..24121)
                     /gene="murA"
                     /locus_tag="VAH76_000921"
     CDS             complement(22853..24121)
                     /gene="murA"
                     /locus_tag="VAH76_000921"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO1385568.1"
                     /translation="MDKLLINGGKALHGEVVISGAKNAALPIMAASLLASDHVTISNV
                     PHLKDITTMMELLGQLGAHLIVDEKMNVQVDSSQVNEFVAPYDLVKTMRASILVLGPM
                     LARFGKADVSLPGGCAIGTRPVDLHLKALRAMGADITVKNGYINARCKKGRLQGKRLM
                     FDTVTVTGTENVLMAAVLAEGITTIKNAAREPEVVDLANFLIQMGAKIRGAGTSTIEV
                     EGVESLNGGTYSVMSDRIEAGTYLAAGALTRGQVTVKKVRPDTLLSQLCKFEEAGAEL
                     TIGEDWVSLNMHNKRPQAVNISTAPYPAFATDMQAQFMAMNSVAEGSSTIIETIFENR
                     FMHVQELQRMGANIQLNGNTAIVHGVEKLTGAPVMATDLRASASLILAGLVAEGETVV
                     ERIYHVDRGYERIEEKLSLLGADIKRVSDR"
     gene            complement(24114..24359)
                     /locus_tag="VAH76_000922"
     CDS             complement(24114..24359)
                     /locus_tag="VAH76_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEO1385569.1"
                     /translation="MLSNEEIEQRLNEIDDVFYVKVEGDGYQYQVTVVTDIFLKKSKV
                     ARQQWVYAQLKDFITTGRLHAISMKTWTKEEWGKQNG"
     gene            complement(24749..25030)
                     /locus_tag="VAH76_000923"
     CDS             complement(24749..25030)
                     /locus_tag="VAH76_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEO1385570.1"
                     /translation="MEINNFKPGAELTFKSVVSVRDKLYKALMEDTRERFCLDLSEVT
                     HCDSAGLALIIEARKLCKQNNKVFDVIGISPETQSLAEFCGVKTILQRS"
     gene            complement(25033..25641)
                     /locus_tag="VAH76_000924"
     CDS             complement(25033..25641)
                     /locus_tag="VAH76_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO1385571.1"
                     /translation="MRVFKTILLVVLMTLSQAMVAQTSPIPMLERAANQIISILKENK
                     ARLKSNPEIIYKAVEENLLPNVDVAGMSRSVLGRQAWSKATEAERVEFSKAFTRLVIR
                     TYSSPLAQYSDETVQFLPLRGSLNSRFIRVNSVIVRSAGQNIPLSYSLVSKNGKWKIY
                     DISVEGVSLLQSFRSQFAQALQHSSISQVIKEMQQKQIKKAS"
     gene            complement(25649..26125)
                     /gene="mlaD"
                     /locus_tag="VAH76_000925"
     CDS             complement(25649..26125)
                     /gene="mlaD"
                     /locus_tag="VAH76_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEO1385572.1"
                     /translation="MKKQRYVDISVGIFMLLGLLALLVMAMKVSNITDFMSDKTYRVT
                     ADFTDIGGLKVRAPVTVAGVRIGEVSRIELQPGELNARVTMTLNGDKKIPYEDASARI
                     LTEGLLGSNYISIVPGFDEDGDTDHPYLRDGDTIAKTQEAIILENLIGQLLFNIKK"
     gene            complement(26126..26908)
                     /gene="mlaE"
                     /locus_tag="VAH76_000926"
     CDS             complement(26126..26908)
                     /gene="mlaE"
                     /locus_tag="VAH76_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEO1385573.1"
                     /translation="MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLR
                     YQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKFGASSQLGQLLALSVARELGPVI
                     SALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
                     FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVY
                     QGFECVPTAEGISHATTKTVVYSSLAVLGLDFLLTAMMIGDW"
     gene            complement(26901..27698)
                     /locus_tag="VAH76_000927"
     CDS             complement(26901..27698)
                     /locus_tag="VAH76_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO1385574.1"
                     /translation="MPDNWVEIKNLFFSLDDRCIFDGIDMVVKQGKITAIMGPSGSGK
                     TTLLRLIGAQLRPDSGQIYVNGQNIHQLSRKSLYETRRNMGLLFQSSALFTHLSVFEN
                     VAFPLREHTELNASMIRNIVLMKLEAVGLRGAAHLMPAQLSGGMCRRVALARTIALDP
                     VLMMYDEPFTGQDPISMGVLVRLIQRLNQLLHTTTIIVSHDVEETCSIADYIYLISGG
                     KIIGQGTPDELKRSIEPQIRQFMHGEADGVVPFHYPAKPYTEELLNA"
BASE COUNT         8964 a         6070 c         4842 g         8037 t
ORIGIN      
        1 tttaatggac tccaattttg tcgaactgca acctgaacga atttttctta tggaaaatgg
       61 tgaacaaata tttgtttcca atctaaaata ttatttcact cataattgta ataggtacag
      121 taatcaccgc tgcttacaac cgcaagcttg aagctctttt gacgaaggag acatttttgg
      181 gatgagcaca ttttattaaa ttattacttt tagatacttc cttatgacag caagcagata
      241 atatttcaga ctgctcttca accataagga ggtattcatg cgggaaatct attattcccc
      301 aagcaaatcc agaaaactaa agtatcatta attccaatcc ggaagatgag ctttcaaaat
      361 aactcactac catggcatca gtgactaact ccaaactgga cggtctccat tgaggatttt
      421 tatctttatt aatcaataaa gagcgaactc cttcataaaa atcactacct ttcataaaat
      481 gactggcgag atcgaaatcc atttttaaac attctgctaa agataagccc ttggcttttt
      541 ggatttgtgc aagggttatc ttaaggctca gaggcgattt ttgcagcaaa gtattatcca
      601 ccccaagagc ccacacaccg tctgtgctct gtaaagactc tcttattagt tcaacactag
      661 gcttggagaa acagacatcg attaaaggtt tgatttgact tgcctcggtg ggcatggcat
      721 cacttgcaaa agaagaaatg cattgatcga cacgattgaa agcgtcttct gataaatctt
      781 ctctctttaa tgcctcaatc attgactgca tttgctctgc aaagacaatc tgcttcacta
      841 accctgcctt cctggcatca tgaggaccca atttatttcc ggttaacccc aaatagactc
      901 ctaaaaaccc tgggcatttg tttaataaat agctggcacc aatatcagga aaaaaaccaa
      961 tccctgtttc tggcattgca aatacaaaac gctcactggc aacagggtgg cttccatgta
     1021 aggaaatacc gacacctcct cccatagtaa tcccatccag caaagaaata taaggtttac
     1081 caaaatgatg aataaaatgg tttaacctgt actcatgcca aaagaattgc atctgctcag
     1141 agtctttact acggcccgca ttatatagcc aacgaatatc accacctgcg caaaaagcat
     1201 tgcccggaac agcttgtacg acgactgctt taatagaatt atcttctttc caaattgaca
     1261 attgcttttg caatgccatg atcatggtta aagttaaagc atttaaagcc tttggtcgat
     1321 ttaaagtaat gaatcccaat tgaccttcct gagaaaacag cacctcttca gtcataatta
     1381 aaccccttta aaatctgcgg ttctcttgtc tagaaacgca gctactcctt ccttcttatc
     1441 ttcagtggcg caaacttttg caaaatgaat tgcttccaaa tgaagtgcct ctgtgagtga
     1501 taaatcataa ccatgatcta tcacttccat tacactggca actgctagag gagccatgct
     1561 taatattccg tttagaatat tttttccttg agtcaacaaa tcctctggtg ctacaacttc
     1621 actgactaac ccccagttta aggcagtctc cgcattgata aagcgcccag tcaaacataa
     1681 atccatcgcg cgccctttgc caattagcct ggccaatcgt tgtgtgccgc catacccggg
     1741 aataacacct aattttacct caggctgacc aaacattgcc ttatttgaag caattctaag
     1801 agtcgcagat atcgctaact cacatccgcc accgaaagca aaaccattca ctgcggcaag
     1861 tgaaggtttc cccatcgttt ccaaaagtcg aaaaacctct tgtccctggc aagcaaattc
     1921 ataaccagtt tgagcgttac attcagccag tcgattaatg tcagccccag cgcaaaatgc
     1981 tttaccattt cctgttaaca ataatgcttt gactttggga ttggatttcg cttgaaagaa
     2041 taattcattt aatgcgctca gaacatcagt gcttaaagca ttgagcttct ctggcctatt
     2101 caaggttaaa gttaagattc cgttattatc tatatcctgt tcaattaaat tcatcaataa
     2161 tcccctatga ttactctata aaatactctt catccaaact tgctttcgct ataatttcac
     2221 gcatgatttc attagtgcct tcaagaattt gatgaactct taaatctcta aatattcttt
     2281 caatttgata atctcgcagg tagccataac ctccatgcaa ttgcatggct ttatcactaa
     2341 tacgaaatgc gacatctgta gccaagcgct tgcccatagc acaatacatt ggcgcattgg
     2401 gatcatcatt atccattgct gctgcagccc tataaaccat cagccttgcc gcttcataat
     2461 ctgttaacat atccgcaaaa taaaaacgaa gagcttgcat ctgagttaaa ggttttccaa
     2521 actgtttgcg ctcatgcata taagattggc tcattcgcag gcaagcaagg gcccctccta
     2581 atgagcagga agcaatattc actctccctc cgtttaaggc attcagtgca attttaaaac
     2641 ccataccttc ttcaccaaca cgattggcaa tgggcacacg acaattttca aaatatagca
     2701 tacaagttgg ctgatttcgc caacctaatt ttttctccag tttgccaaat gtcagaccag
     2761 gagtattctt ttcaattaac aaacaactga tgccatgatg agactcatca cctgtacgta
     2821 ccatacataa atacacatca ctaacacttc cacccgatat gaatgctttc gcaccattaa
     2881 gaacataata ttcaccctct tttaccgcgc gagtttttaa agaggctgca tcagatcctg
     2941 attctggctc tgtaagacaa taactggcta tcacttccat actggttaat tttggtcccc
     3001 atttcttgcg aatctcttgg ctgccatagc ggtcgaccaa tgaagcgacc atattatgga
     3061 tggagagata tgcacttgtt ggaatacagc ccgtagcgag ttgttcaaag atgagtgctg
     3121 catccagacg agagagctga gcacctccta tgtcttcgcg gactaccatt cccgccatac
     3181 ctaattgcgc cgcttctctt aaaatttcga ttggaaagta accttgttca tcccagaaat
     3241 ctgccattgg agccagtttg ttacgagcaa actcggaggc catttctcga aatgccaaat
     3301 gttcatcagt gaaattaaaa tgcatagacc atccttagat ttattgattt tagtcattgg
     3361 atttatttat gacgctttct taaaaaatga tattattgcg aatttttagc gcccaggaaa
     3421 gataataaga ataattacat gggaacttta gcgcaaccat cttttataaa tccaaacgca
     3481 ttagctgttt taaaaaaata ttttggattt gattccttca gacctcctca ggaaaaaata
     3541 attgaggatg tcatttctgg ggaagatgta ctggttttga tgcctactgg agggggaaaa
     3601 tcgctttgct atcaaattcc agccattgtt cgccgtggtg ttggcattgt agtatctcct
     3661 ttaatcgcgt taatggaaga tcaagtaacc gctctcaaac tgcagggtat tcgtgcggct
     3721 tactacaact catccttaac cgccgaagag gctagaaatg tattaaatca attacatcat
     3781 aatgaattgg atttactcta tattgctcca gaacgtctta ttagtacctc gtttctggat
     3841 cgactgtcag aatgtactat ctctttattt gctatcgatg aagcacactg tatttcacaa
     3901 tggggacatg attttcgacc tgaatacgcc tgtttaggtt tactgaaaac taactttcct
     3961 gacattccta tgattgctct gacagctact gccgataagc aaacacgtga ggatattgtt
     4021 accaagttga attatcaacc caaaaaatac gttgtttcat tcaatcgacc gaatatccat
     4081 tacaaagtag ttcctaaaac aaatgcagtg aagcaattaa atctgttttt acaatctgat
     4141 acccagcaat ccggaattat ttattgtgga acccgccata cagtcgaaca tcttacagcg
     4201 aaattacaag aacttggatt gaaagcacgc gcttatcatg caggcctgtc tcatacggag
     4261 cggcgtgagg tgcaaaacct tttcagatac gatcgcattg atattgtggt agctacaatt
     4321 gcttttggaa tgggtattga caagcctaat gttcgctttg ttgttcacca tgatttaccc
     4381 aaaagcatcg aaggttacta ccaggaaaca ggtcgtgcag gacgtgacgg attaccggcc
     4441 aaggcccttc ttctttatga tgctgcggat agtgcccgtt tgcggtcctg gataatgaac
     4501 atccctcttg atgagcaaag acatatagaa accaataaat taaatcatat gcttgctttt
     4561 gctgaagcgt cgcattgtcg tcgtcaaatt ttattacgct attttgatga accttctcat
     4621 acagaatgta aaaactgtga tgtctgcgat aacccaccgc aaacagccga tgcaacagaa
     4681 gatgcgcaaa aatttttatc gtgtatttat cgattaaaac aaagctatgg tttaactcat
     4741 actattgatg ttctgagagg tagtttatct gaaaaaatca agcagtctgg gcatgagcat
     4801 ttaagtacct ttggaattgg gaaagagaaa tctgccaatt attggaagca tcttgcctgg
     4861 caattaattc ataaagatta ttgtttacag gacatgaatc attttaatgt gttacgcttg
     4921 accccaaaag cgattcccct cctaaaaggt gaagagagta tttcattaac catccctaac
     4981 aatgatctga atagcagtaa aaagaaaggc aagcagagtc cttcctttaa acctgaaagt
     5041 aacccattat ttgaaaaact tcgtgcgtta agacgtcagt tggccgatga agaaaacaaa
     5101 ccaccattta tgatattcag tgatgctaca ttacatgaaa tggcccaatt aaaacccaaa
     5161 aacagtaagc aactactgaa tgtatctggg gttgggcaac ataaattagc tcgttatggc
     5221 cattattttc tcgaagcctt aaacgaattt tcagaagtcg agtgagattc cgttatgcgt
     5281 aaattaccaa gaccctttta cgaacgtgat accattctgg ttgcaaaaga actcttaggt
     5341 aaatatttgg ttcatcatga tggattggaa gagaagattg ggcgcattgt agaagtcgaa
     5401 gcctaccttg ggcaacatga tttggcctgc cattcatcaa aaggattgac taaaagaact
     5461 aaagttatgt ttgggccagc aggctatgcg tatgtttatc taatttatgg catgtattat
     5521 tgcatgaacg tagtcaccga aaaggagggg ataggttcag cagtattaat acgtgccctg
     5581 gagcctatca aaaacattca agacagaact caaggaccag ggttattatc caaagctatg
     5641 cgcattgact ccaaactgaa tcatcgtgat ttattaagca atgattttta tattgctgag
     5701 ccaaacagtc caactgattt taccattata gaaaaaccca gagttggggt tcattatgct
     5761 aaagaatggg ctaatgagtt attaagattt tatattaaag acaatcctta tatttcgaaa
     5821 acctgattag gcctcgtgca taaactgtct ctttcatttt attgccgcat tgcaaagatt
     5881 tctgtgatgc tcagggtatg taaaaagcgt attatcgtat taatgagccc attattatta
     5941 catccctttc ttcaagcatg tcctgtcaat ccccggatcc aggctttgtg cctaatccgg
     6001 ggtaatttct tgaaataact ctactctaac ccaattgcct tgggcgggca gatataggca
     6061 ttacccagat tagtcacatt ggtttgttct gaaccgccgc tcataaaacc accgtgacga
     6121 tcaaatgaac cactcatgga acccgttaca ccggctcggt tcgtcaccaa ttgaatgtaa
     6181 tttgcccctt ttttgcttgc tttattcttc agatcattca ttgctccttc ttccaaattg
     6241 cgattggaag taaagcctcc ggtaaagaaa ttaccctgat tgcctacaac ctggcccacg
     6301 tatttgcaac ctttaggtgc cggattcggg cttgcaatga cacgttcggc ctgaggatct
     6361 gctggaatag ctgcgcatcc ggtaatcatc aaagtaataa cagacgcaga tagtagtttt
     6421 ttcataatta cacctttttg ttatcaacaa aacaatgaaa tgttaacaac tcctgtttga
     6481 ttaaacatta caacccactt ttaaatgcac ttgataatat ttcactttat tatcatcaat
     6541 gaaacctctt gtttctacta cttcaaacca atccaatttt ccatgcgttt tagctacttg
     6601 ctttattgca ttatcaatcg ctttttcaat tccttcttgc gaagtcccaa ttaactccac
     6661 gatttggtaa actttatctg tcactcttct ttccccctct attaatttgt ttctcagctt
     6721 ttgctattgc cttgcgaata gccaatgctt cttcggactg cgctggcgcc aactggagta
     6781 gccgttgcca gtaattaatc gcagcttcat aatcatgact taagtaagca tgcatcgcaa
     6841 gcatggctaa agcatccggt tgcctggggt tattttttaa taaggtatga aaaatttcta
     6901 aaatatcagg attgaactgt tgattactca attgccataa actgtgggca taattgactg
     6961 caaattgctc gtcttcaggt ttaagctggt gggcttttgc aaaagcgagg gaagcatttt
     7021 ggttatcctt ttgactgctg tataatctgc ctaacaaata ccaacccttg gcactttcag
     7081 gctgcgcatc caacttggcc ttcagcttgt cgattaattc ctgagggctt ttaatcgatt
     7141 ttagcatcga ttgcgctagc tcctgagcct catttcgatg tagatattct tgccaccgag
     7201 caaaatctcc ccaaaaatag taccctgtaa aaaccattat aaaaataatg ggggcgagca
     7261 acaaacttac caaacgatta cgccgcaaag gatagataat aagagtgcat gcaaaaacgg
     7321 ctaaaacaac caataaaata attaaccacc attcattcat gagaatttcg cttaaaacat
     7381 gtccgccaaa aaataataaa gccaatcaat aaaaacagga atgggccaaa ccacaataaa
     7441 agagtcacgc tcttaacagg cggtttaaat agtataaaat ccccataacg tgcggttaga
     7501 taatccatta tttcactatc gcttttgccc tctttcacta attcataaac ctgaaaacgt
     7561 aaatccttcg ctaattgagc atttgaatct gccaaatcct gattttggca taccaaacaa
     7621 cgcaaatctt ttaaaagatg gttaaattgt gcttcttttc ttgctgagtc caatggatac
     7681 atactacttg caaacgcaac ggatactaat agcagcaata aaatcacctt aaaacagaag
     7741 tggaatactc tcattatgat ttctccaaat tcctcattaa aggtaaaata tcagttaacc
     7801 aggttttctg attcattatc ccgacatgcc tgtagcggac tattcctttc ttatcaatga
     7861 caaaagtttc aggcgcccca tatacaccta agtcaatagc gactttacct tctgaatcct
     7921 ggcctatgag tcgataaggg ttcccccatt gtgcaagcca tgctctcgcg tgctctggat
     7981 tgtctttata atttaaacca tagataggta cgccttctct tgcgagttgc aacataaata
     8041 cttgctcttc aacgcaagca tcacaccaac ttgcccagac attaagcaaa acgacctgat
     8101 tatgaaagtt cattgcacta aaagtagaat cgggattttg caactctggt aaggcaaagt
     8161 ctggtaatgg ctttccaatt cgagctgaag gcaaatgatg aggatccaaa gataaacccc
     8221 gccagagaaa aaaacataaa agacaaaaaa tagcgatagg aataacacgc caccctattt
     8281 ttttcatatc ttgatctcct gccctgccat taccgttact tgcctttttc tgtaatacct
     8341 tctatctgtt aatgccaaaa cgcctccggc taaaatcata aatccgccag cccatatcca
     8401 gcggaccaac ggtttataat aaattcggac cgaccaggca tcctcagaca gtggctcccc
     8461 caaagcaatg taaatatctc ggaaaggagt cacatcaatc gcagactcag tcattggcat
     8521 ttgtccaatg gtatacactc ttttttcagg aaaaatttgc ctagttttat tctgatattg
     8581 tattgaaaac tcagcccttg tccctttgta atttgggcca ttcaacggag tctgattaat
     8641 gaatccaatc tgataatcca gcagactcac ttgcttgcca ggagacatac gtacatcatc
     8701 ttgtacgcca taagctgaag aaacagcaat gccaatcact gttgctgcga caccacaatg
     8761 agccaaaacc atcccccaat aagcctgtcc aactcctgcc acccctctat ctctgacttt
     8821 gtgatacgcc gcattagcag tactcaacaa aacccagaat gccaaaatta atcccaaaag
     8881 ggctgtaagg tgtatttcag ttgaggtaca gaatagtaaa atgacaggca atatcagact
     8941 aagcaaaaaa atacgattta gttttctgaa gacatccctc caacaatcat tctgccattt
     9001 caaacggaca cctaacccca ttagcaataa taaaggaatt attaatggaa caaatactgc
     9061 attaaaatag ggcgctccaa cggataattt gcctaaccct aacccttcca ccagcaatgg
     9121 ataaaccgtg cccattaata ccgtcagcat aataaccact aaaaacacat tgtttaacaa
     9181 caacgcactt tctcgggata ataagccagg atgtttattg acttgcaagg tttgggctct
     9241 gaaaagaaac aatgttaaag aacccccaat gacgaataac aaaaatccca gaatatataa
     9301 tccccgctga ggatcaaccg caaaggcatg aactgaagtt aaaactccag agcgaacaag
     9361 aaaagtacca attaaactta aagaaaaagc agcgattgca agcaacatgg tccacgcttt
     9421 aaattgctgg cgctgctctg taactgccaa agaatgcaat agtgccgtac caaccagcca
     9481 gggcataaaa gaagcgtttt ctacaggatc ccaaaaccac caaccacccc agcctaattc
     9541 tctataagcc caccaactgc ccaaagtaat tcccgcggtt aaacaacacc aggcagccag
     9601 agtccagggt ctggtccatt tagaccaact ggtttctact ttccctgccc acaaggcggc
     9661 aatagcaaaa gcaaaagcga cacaaaaccc tacatatccc atgtacaaca taggaggatg
     9721 gaataaaaag ccaggatctt gcagcaatgg attcagatca cggccctgtg tattcaatac
     9781 ttgaaattgg cgcaagaaag gattggaagt cattaacaaa aaaagaataa aaccaatgct
     9841 tagccaacct aaaataacta atacccgagc gcgcatttcc gtatcaaggc ttgagctaaa
     9901 aaaacaaacc aggatcatcc aaaaactaag gatagcaacc cataacagca tggaaccttc
     9961 atgcccaccc cacacagcac aaaatttata aaaccatggg agcaataagc tggagttatt
    10021 cagcacatag atcaccgaaa aatcatccat caaaaaacag gccgtgagac ataggtaagc
    10081 acatgccaca aataaaaatt gcagcacgac ataattcctg gaggaatcaa tccagtcttt
    10141 ccgattgaat tgcaaaccag ctaatgggat caaagctaac atcaaagaag ccactaaagc
    10201 caaaatcaaa aaaaacagtc ctaattccgc tatcattgtt tcaccttatc agctaaagca
    10261 gactttactt gaggtggcat ataattggca tcgtgttttg ccaaaacttg tatagcatga
    10321 aaatgatgat tatcagttac ttctccttct gcaactattc cttgcccttc tcgaaataaa
    10381 tcgggaagta tccctgaata ggtaacaaca atcgtgtttt caaaatctgt aattttaaac
    10441 tggacatcca aacctttttt ggcacgtact atgctgtttg cttccaccat acctccaact
    10501 cggatgtggt gttttgctgg tgcttctccc gcaacaatct gcgtgggggt gtaaaataaa
    10561 ctgatatttt gtcttaaggc atatagaacc aaggcagccg ctgcagataa aacagacagg
    10621 gcaaacagta agataaataa tttgcgtcgc cgtacagggg tcatttcacc tatctcttaa
    10681 accaacgttg taattttttg cgggtacgag ttctttgcca ctttattcct aaaatattca
    10741 tgcccaaaac cacgcaaacc aaaccataag caggccaaac atagagagaa taaccgccca
    10801 ttgccaacca ttcaaaaaac tgattcatca gactcttcct caaattgaac cttaacccat
    10861 gattgctttc tctccctaaa taagacttca ttacgtgctt tttccaaaat gatccataag
    10921 gaatacaaga aaaaacccag caaagtgatt aacaaaggat acaacatact gggagcaatt
    10981 ttgggtttgg caaacacaga taaagtcgca ccttggtgta aagtattcca ccaataaact
    11041 gaataatgaa ttattggtaa atcaattaag cccaccaaag ctaaaattgc tattatttta
    11101 tctccatctt ctttattttt taccgcttgg taggtagcca gaattgccag atagagtaac
    11161 aaaagtatta actctgaggt caggcgcgca tcccataccc accaggcacc ccacatgggt
    11221 ttaccccaaa tgctccctgt aattaaagca agaaatgcca tgcaggcacc taattgcgcg
    11281 actttatgaa tcaaaagccc tgccattttg atacgccaca ccaacaataa aatggccaga
    11341 aaccccatcc aggcatacaa tgccattgat aaaaaagcgc tgggcacatg aacaaaaata
    11401 attcggtatg catccccttg ctgataatct ggtggagcaa ataccaatcc ccaaaccata
    11461 ccaatggcta tggtagctaa agcactgact gccagccagg gaatgagacg tcccgcgtag
    11521 ttataaaaat tttttggcga tgccaactga tacaatatct tccacatagg aaacaataaa
    11581 atcaaaaaga aggattctac cacgcatcaa aacaatcaca aaacaatcac aaaatttagt
    11641 tccaaactta gggtctgttg acatttcgct cgcttgagtc gaaaaacctt gattttcgag
    11701 caccgtagcg gagcatacat agaagtatgt gagtaacgga gcacagaaaa tcaaggcttt
    11761 ttggccaaga tagtagaaat gtcaacagac cctagagcgc cgaatgattt aaatttacgc
    11821 agctaaattc atagcgatgt gtaaattagt aatcatggca gttcggttca aatcatactc
    11881 attaagtctt aatccgatat atcttgctct tggtccttta cgattacaaa tggtcgcaag
    11941 agcatgctct actttaacac gttctcttgc ttcctgacga ccctcaggcg acgtaacata
    12001 atatttcaat gcctgcagca ttttctcttg gtcatgaatt tgaatcactc ttccagtctt
    12061 tgagtcactg catttgttct ttatgggaca agcaccacag agtttggctg agaatttggc
    12121 catcaggctt cctggtttgc ctgtgatttt ttgctgtttg ccagcaggac aaatgaccat
    12181 tgaattttgt aaatcaatga taaaatcttt tttcccatat tttccatgtg ttgctggatt
    12241 ccatggctta gcaatgatct ttttaccttg tgaaaataac tcagcagtcc atgatgcaga
    12301 aaggtacccc cgatcaatac tctttgaggc aacctcccca tatttttcaa cttttggctt
    12361 aagccattgg cttgcttcat gttctggttc attagctgga cgtacgcttg ttgccaaaat
    12421 taacttatga tcaagatcaa tagcgatatg ctgtttaaat ccattgattg tacgagagct
    12481 tgatttacga ccatgacgca tatcactatc tgagatagag attcttctat ctgtagaaac
    12541 gcctctgata attttggaac caccatctgg atcaggttca atattctgat ttaaaacagt
    12601 atctaataat gacaatgctt cttttagata ttgattgttt atcaatgaat cagattgccc
    12661 gcttagccag cgttgtaatg cttcaacatc tttaagcaat gtattaatgg ctttttgttt
    12721 ttcttgaata tccgaccagt caatatccaa ggcagccttt atactactac ggccaattaa
    12781 gtatgtgccc gcttccttga taatctcaat ttcatcaact tgctttattt gagcagcaca
    12841 atcaaccact aactctaagg catgaccaat caaattgaag gtatcttcta ctcgaccagc
    12901 gccttctaat ggcgccgaat ctagcgctat tcttaactgc ttataacaaa atccaccaac
    12961 ttccttggct acctctaccg tttgctcaag cagcattgta tccaaattat gttttactaa
    13021 tcgatgccta aaatcacaca atgtaccttg agaaaagatg ctttcttctg ctccaagaca
    13081 gcctaaaacc atttgccagc gttgatcaaa catcgtttca agaacagctg aagaatccga
    13141 taattgctca taagcttgta atatcgaaac cattgcaaga agcgtagggc taatcaatgg
    13201 cttaccacga ggataatctg catacatcga caataatttc tggcttatat cagcagtaaa
    13261 aataagatgt cgattttttc ttaaaaaaat aaataacttc ccattttgtt ttaatttgct
    13321 gcagatcttc tcttcttttt ttgatagctc aatggggtaa atccagtttt cctgctttat
    13381 catgcacact cctttacact tgtctgcatg aatttgatca taaaatttga acaatgtcga
    13441 tctggttaaa aattaatcga tcggcgctct agtctatatg actaatacgt atcactgccg
    13501 aaatcgcata gggtagaaaa ccaactgcca atagagacat cgccaataat aaagccagat
    13561 agccgctgac cggcaaacct tgcatcgcaa cgctcaaagt accactacca aatatcagta
    13621 aaggtaaagt taacggcaat agaatcaatg ccatcaaagc cccgcgctga tttatcccta
    13681 taccaaaagc tgctgcaagc gcacataaaa ataatatggc aggcgtcccg caaataaggc
    13741 tcaataacaa tatacatgtt tcccaaacat tgaatgaaaa taatattgca atcaaaggag
    13801 ataataataa tagcggtgtc acagatatca accagtgtgc cataactttg gctgtcacaa
    13861 ggagatttaa tggttgccct gaaactagcc actgttcaag aaccccttgt tcataatctt
    13921 gttgaaacaa ccgttctaac gaaagaagca tggataataa aatggccatc cacactaatc
    13981 cgggagcaaa catacgcatc aacatcaatt ctggtcttag agtaagagga aaaataaata
    14041 acaacattaa aaaaaataaa cacgaattaa ctaaatatct gatttgccgc acctggatta
    14101 atatttcacg cctgaactgt tttccgaata attgaaatgg ggaattcata gatgatattc
    14161 ctcataatct gccttgttta aaggcaaatc ctgatgagaa gtcaataata cagcaccacc
    14221 ttttgccctg tgctcctgga tttttctgat aatcgtaagc aatgataact catcaagagc
    14281 aaccaaaggt tcatcaagca accataattt tgcttttgac atccataatc ttaataaagc
    14341 aacttgcctt ttttgtcctg aggatagtaa tccacatgga taatcaataa ggtgttctaa
    14401 actgaataat ctgcataatt ctgtaatgcc cactgcacca ggagaaaaat gtatgtcata
    14461 caaacaattt tctcttagcg taagataggg attgatccct gatctatgcc ccacaaaaca
    14521 taattgcttt tgataggtgc atagatcttt ttttatagat cgcctttcga agagaatttc
    14581 tcccttttca ggattaagca aacctgctat taatttaagt aaagtcgttt ttcctgctcc
    14641 attggaacct tttaaatgca ataagcctcc agcaggaaga tgaaaagaaa tttgttgcaa
    14701 caatggctgg tcatgatagt caaaatcaag ttcaatcaca tcaagcataa aacactttat
    14761 ttttagatac actcggctaa ctataacagg acataagaaa aatccccaat gccttcgtta
    14821 aaagttattt aatttttgct tgcgaagtga gtggatgatc gccataatct gctttatttt
    14881 ttgcaaaggt atatgatgga attccttagt gaatatggca tgtttctctt aaaatgcatc
    14941 actctcgtca tcgcattact cattcttctg gcaggtatat tttctatggg tcgcaaaacc
    15001 aaacccaaac tggaaattac ctctcttaat gaagaatatc agcacctgaa tgccttaatg
    15061 aataaagaga ttttaggaaa aaaacctgga aaaaagaaaa aagataaaac caagcgcccg
    15121 gttctttatg tcattgattt cagcggtgat atcaaagcaa cccaagttga acaactaaga
    15181 gatgaagtca catcggtatt aagtatagca aaacctgagg atgaggtttt agtaaggttg
    15241 gaaagccctg gaggggccgt caatggttat ggacttgctg cgtcccagtt acagcgaatc
    15301 cgtgataaaa aaattccttt gactgtcagc atagacaaaa tggcagccag cggcggatat
    15361 ttaatggcct gcgtagcaaa taaaataatt gctgccccat ttgcaataat cggctctata
    15421 ggcgtagttg ctcaaattcc taattttcat cgctggttaa agaaaaacaa cattgatgtt
    15481 gagctattaa ctgcaggaga atacaaacgc accctaacac tttttgcgga aaatactgaa
    15541 aaaggtagaa aaaagtttca ggaagatttg gaaaaaattc atactgcatt cagagagtat
    15601 gttcttaaaa atagaagtca actcgatata gataaggttg caactggtga acattggata
    15661 gcaaaagatg cttttgatct ccggctcgtt gataagttag ctactagcga tgaatattta
    15721 atagaaaaaa tggcggagtt taatgcattc aaattaactg tccatgccaa attgccaatt
    15781 ataagcaagg tgcttaaacc ggcaatgaga ctcattcatc cgtggattta aaaattagta
    15841 ctgaaacagc ttgggaacgc cattgaaatt caataccgtt aaatcaggca gcattccata
    15901 ggaaacgaga tcaattttat catgggctta aagctgatga tggtagcgga atttaaggaa
    15961 ttaatgagca aaaattttca aaatccgcca ttaaccaaaa tttgaatctc ggtatataat
    16021 taaagaataa caattttctc tgaggagtaa gacatgagtg atttcaaatc gaagttacca
    16081 gatcttaatg aattaacatc aatgaccgcg aaattattta aagggattaa aaccagtgtc
    16141 agtgaaataa ttcaggacta taagcaaaaa agagcacagc ctgttagtac tgaagagaaa
    16201 accacagaaa ataagccagc tgaaagcaag gccgaaccaa aagcagaaag caagactact
    16261 gaaaacaaaa ctgagccaaa aacacaaagc aaacctgaat caaatacaga agaaacacag
    16321 gaaaaagaga aataatcctg tgctagcaaa taagtgatac ggggtgagct gtatctatct
    16381 atcgttccat tctttctggt cgagttaagc catatttgcg cattttttca actaaagttg
    16441 tccttctcat gttgagataa ctggccgcat gagcaacaac ccaatctgac tcatctaagg
    16501 cctggctaat caaggcaagt tctgttttaa ccaaatgctc ttttaaatca atgccctctg
    16561 cattagctac tgactcctgc tttattattt gcaacaaagt ttcacgttcc gcccccgatg
    16621 cctctggaca agatggttta ttttcacctt gaaaccgatg aggtaaatca ctcttgccaa
    16681 taataccatt agggtaaaga atagataatc tctccaccaa atttgctaac tccctcacgt
    16741 tacctggcca gctgtattcg cataaagcat ccatagcatc gggcattaat cgaacggtag
    16801 gtctgccctc tccttcaatt cgagaaacca gctcattaaa aagcagtgat aaatcactac
    16861 ttctattacg tagtggaggc atttcaatgg gaaatacatt caatctgtaa aacaaatctt
    16921 ctctaaattt cccttcttga atagcaattt ctaaatttct atgtgtagca gcaatgatac
    16981 gaacattgac ttcgatgctt ttatttgaac ctactctttc aaaacaacgt tcttgtaaaa
    17041 ctcttaataa cttaacctgc atagctaatg gcatatctcc tatttcatcc aggaatagag
    17101 ttcctccatt ggccaactca aaacgtcctt gccttgatgt aatcgctcct gtaaatgcac
    17161 ctttttcatg accaaataat tcactctcca ataattcccc cgggattgct ccacaattga
    17221 tagggacaaa tggtttattt gctcgggatg aaagagaatg tatgttgcga gcaacgactt
    17281 ccttaccagt acctgactca cccaaaatca acacactggc ctcagtatca gctacttgct
    17341 caattaattt gcgtacttga cgaatacctt cactattccc aaccaagctt ctaaataaag
    17401 gagtcttatg ggtacctgca gtgataaatt taactgcttc tttagctatt tgacacctat
    17461 ggagcgattt aagcattttt gcatagctga atggaaatga taaacaatca acgatatttc
    17521 gatccgcgca ttcagatgta gaaagcacag catcaataac caatattgga gtttttggaa
    17581 actttttaac taatgtatcc aattcatcca tcattttttc aaaagaagta cacgctccaa
    17641 gaactatcac acttggatta ggattaacac tcaattgcca attgccatat tgagtgacct
    17701 cagagaattg tcctataaaa tcaaaaatag tccgaagctt gtcacaacgt tccttatcat
    17761 catcgataat ataaatcctg tcattgctcc tcataaacct atccttaagt ttgagtcatt
    17821 taatttaaag tatcggacac ataatgaata actgtcaaat aattgtcaca atttatttta
    17881 accgtcataa aatctatcaa attaggccgt atcgctataa tcaaaaaaat actaacctat
    17941 ccagatgcta attaaaaaat catagtcatc accagactaa gtatttgatt tatctgtaac
    18001 aaataatcat cttaaacgca ttacactgat taccaaaaca taattcctga cttcctatgg
    18061 tcttcgtagc actcacttga gcctgcttca cgaaatcctt ggcttgcata tatcccatac
    18121 gattacagaa cgaattggca gcttgtttac cacagccttt attgcgctcc atacaccaat
    18181 ccactcggta atcattataa cggggtacgg caaatttttt ctctcgataa tggtaaggtt
    18241 tgggaggagt atgcgataaa ccaatagcac aagctattgt cataaaacca ttacaacgcc
    18301 atcctttgca ttgagccttg ctacccaagt aattagttaa accgacgtta taggcaatga
    18361 cattttgagt ggagtaatca taacccagca tttggcaata acgattcgct acctcctttc
    18421 cgcactcttt cccatctaaa gtacagtaat caagtcgttg ccctaaataa gtaggatgcc
    18481 aaaaatttct aaatgccaaa ttctgcttac ttgaagctgt taactgaccg caaaaagaaa
    18541 aaaataaaag tataaaaacc aggaaatttc taaaattgct cattataaca atccattacc
    18601 aagtattagc acatgttagc ttaggatgac tgggaggcac aagagagatt tattcttctt
    18661 ttactcgtac tgtaatgaac ttttgtctta ttgcattttc tacatacgac tatgatttaa
    18721 aagctgacca aagacaaaac aactatatca agataatttt tcaaagacta taaacaatgt
    18781 tataaatagc atatgaacag aacttatccc tggaatcaag gaaacaaatg tttttaataa
    18841 gaaagtttgg ccaaattgaa tgacagaatt aaaaacactt tgtaacaaag agcatagggg
    18901 cttaagcaaa ttctaatgaa acaagctttt caatcatcac tttaaaagcc catatcaaag
    18961 gctagtcgtt tatgaaaaaa tatttaactt ttatagtgtc aggcttagcc ttaattgcaa
    19021 taatatggtt cattcgccac ttatggataa ttgagactgt caaggttgtg catcctgttt
    19081 atggaacagc agttgaagct gtctatgcca caggaacagt cgaaccaacc gtcatgatgc
    19141 caattgcctc acaaaataca gcccgcctta tcaagttgaa tgcagatgag ggttcggaag
    19201 tagtaaaggg acagatatta gccaacctgg aagatgaaca cctgcaacat aacttaaaag
    19261 aactgaaaga gcatgaaaaa ttagcaaaaa taagatacga gcggaatgct caattagtta
    19321 aaaaaggagt catctccaaa gatgagtatg atcgcacctt gtccgaatgg caagcagcca
    19381 gtgccgcagt acgcagaata gaagctcaaa tcagttattt acaactgata gcccctgcag
    19441 atggccgtat cattaaacgt gacggtgaag ttggccaact aatcccggca aatcaacctg
    19501 tattttggct ttcttgctgt gcccccctaa gagtttccgc tgaagtcgat gaagaagata
    19561 tcgtattggt cagagttggg caaaaagtat tgatcagatc ggatgctttt ccaaatcaaa
    19621 tcttttatgg ccgtgtcaaa agcatcaccc ctaaaggcga tccaatagcc agaagttaca
    19681 gagttcgcat tagtttttca gaaaataatc ctttattgat tggtatgaca gctgaaacca
    19741 atattattgt ccatgaaaag aaaaatgcct tattgcttcc tgtaagtagt atttctgaaa
    19801 acaaagtttg gattgtacag gatgatggga agctctctca aaatacagtg actattggcg
    19861 caagaggatt aaaacaagcc gagattttaa aaggcgtcaa tattaacgac ctggttgtct
    19921 tacaacctga taataagcta aaaaatgggc aaaaagtgcg tttcattctt gtaaaacagg
    19981 aagaataatg aagctatttt taaagatcgc tttaaaacat ttgcttgcac gcaaacgaca
    20041 aagcctggtt tctttgatgg gaattgtatt gggggtagcc ttctttttaa caatttcgtc
    20101 actgatgcag gggtctgaaa aagatttcat caaacgcttg gtggataatt caccgcatat
    20161 cacgatcata gatgaatatc gaaaccctcg tatacaaccc atccaacagc tgcacacaca
    20221 tggcgctatt gaaattcgca gtgttaaacc attaacagaa cctcgaggga ttagagggta
    20281 tgaacaaaca ataaataatt tatcgcttac catcccggga attgttattt ctcctgttct
    20341 aattggacaa gccttaatta attatgcagg gaaagattat tcggtcactc tgaatggtat
    20401 gatccctgaa gagataaaaa aagtttctac tatcgaaaat tatatgactt caggttcgat
    20461 tgatgatctc atagtcaatc ccgatggtat tgtcttggga agcgagctct tgcgtaaatt
    20521 atccctgaca aaagatgata acattactgt gacagcgccc aatggtcaaa tacgcacttt
    20581 caaaattctt ggtgtatttc gcactggccg ttcagatttt gataccaatc aagcgtttat
    20641 tagtattaaa cgcgctcaag cattattaaa ccgacctaac cgctccaata acatcattat
    20701 aaaactgcct aacccctatc aggcctatga cattgccgct caaattgaaa aacagatagg
    20761 ctataaaact atttcctggc aggaaaaatc ggaagatttg ctcaatacac tagtcattcg
    20821 aaatatcatt atgtatagcg tggttagcgc tgttttaatt gtcgctgcat ttggaattta
    20881 taatgttatt tcgactgtag taatggaaaa acacagagac attgccatac ttaaatcaat
    20941 cggtttccaa aaacatgata ttcaatttat ttttatcata gaaggttttc ttctaggact
    21001 ggttggatgt ttgttaggac tgcctttggg aagtggactg atgtacagtc tcatgcaagt
    21061 ccaattcaag ccgccaggaa gctctgaatt gattaatatg ccactcgatt ggagttattt
    21121 acaatttgtc atcgccacag cttttgcgat gtccgcctca atcatagccg ccgcccttcc
    21181 tgctcataag gcagcgcttg ttcatcctgt tgatatcctg cgaggagggg gaatgtgaac
    21241 aagattgtca taaaaacaaa agagctttcg cgtcaactgt caggtgaagt tcccgtcact
    21301 ttagtcaaag acataaatct cgaagtcaga gaaggtgaat ttttagtaat ttctggcccc
    21361 tcaggttctg gcaaatcgtc attgctttat ttgctaggct tgcttgatag accctcccaa
    21421 ggaacaattt ggctgaataa tgaagatgtt tcaacttatg aggaagaaaa aatggcctgg
    21481 ttgcgcttga ctcaaattgg ttatgtcttt caattccatt ttctattacc tgagtttaca
    21541 gccctggaga atgtgatgtt gcctatgcaa cgtctaggca aactaagtct gggggaaatg
    21601 aaaaaccgag ctaccaagct tttaaccagc ctagggttag aggagcaatt atcaaaactg
    21661 cctaaacagc tgtcaggagg gcaaagccag cgcgtcgcta ttgcacgagc tctggccaat
    21721 gatcccatat tgctcttggc agacgagcca actggaaatc ttgataccgt ttcaagtacc
    21781 aatgtgcaaa atatactaaa agaacttgct catgaataca gaagatcggt tatcatcgta
    21841 acacacgata tgaattttgc tgctacagca gacagagtga ttcacattgt cgatggcaaa
    21901 atgcaagaat aaccttactt cttgctagtc catgcttact tcatcagttt catcctgatc
    21961 tattcttcag gactaacgaa aaaccccaag gtcgaggcaa aaaatgtttt taatgaggga
    22021 gtttagataa actaaatcac cgaattaaaa acatttttta acatacagat tggggttttg
    22081 cgtaggtcct gttcttttta aatcggatta ggactatcta taaaccaatg ttccaagtcg
    22141 aaatagtttg caagataaac accgagggat tgaatgccga accgttccgt cgcatgatga
    22201 ccacaagaaa aatattgtat acccaattcc atagcctgat agaacgttcg ttcagaaact
    22261 tcaccgctta aataagcatc aacgccaagt tggtaagctt cttctataaa gtcctgtgct
    22321 gctccagtgc accatgctat ggagtgaatc attttttcat ttcccgcaat atgcacagga
    22381 taccggccaa gtttgtgctc taaaaaactg gaaaactgtt cgctgttcat tgcctttgat
    22441 aattttccag accaaaggag attggcagta ttgttgacct tatgcatctc atgggactct
    22501 attaacagca aattcgccag gctggcatta ttccctagct cgggatgaca atccaaaggc
    22561 aaatgatagg caaataaatt catgttatgg ccaagtagct tagcaatcct tcgccttttc
    22621 attccggaaa taacaggatt ttcacctttc cagaaataac catggtgaac taacagtgca
    22681 tcagcttgct gttcaatagc ttgggaaatc acgtcctctg atgcactgac tgccgtacat
    22741 atacgcttaa tcttgtcttt gccttcaact tgaattccat taggtgcata atcctggtat
    22801 tgagagcaat tcaagaaatc cagtaaaaaa aggcttaatt catctcttgt aatcacctgt
    22861 cagaaactct cttaatgtcc gctcctaata gagataactt ttcttctatt cgctcatagc
    22921 ctctatcaac atgataaatc cgctcgacta ctgtttcccc ttcagcaacc aggcccgcga
    22981 gaattaaact ggctgatgcc cttaaatcag tagccattac cggtgcgcca gttaattttt
    23041 caacaccatg aacgatggct gtatttccat taagttggat attagccccc attctctgta
    23101 actcttgaac atgcataaat cggttttcaa aaatagtttc tataatagtt gaagaacctt
    23161 ctgctacgga attcattgcc ataaattgag cttgcatatc cgttgcaaat gcaggataag
    23221 gggcggttga aatattaact gcctgaggcc gcttgttatg catatttaaa ctgacccaat
    23281 cctcaccaat ggttaattcg gcgcctgcct cttcgaattt acataattga gataataaag
    23341 tatcaggacg aactttctta acagtcactt gaccacgagt aagcgcccca gctgccaaat
    23401 aagtgcccgc ttctattcta tcggacatga ccgaataagt cccaccgttt agtgattcca
    23461 caccttctac ttcaatagta gaagtacctg cgcctcgaat tttcgctccc atttgaatca
    23521 gaaagttagc caagtctact acttctggtt cgcgcgcagc atttttaatc gtagtaatgc
    23581 cttctgccaa cacagcagcc attaacacat tttcggttcc tgtgacagta actgtgtcaa
    23641 acatcaagcg tttaccttgc agacgtccct ttttgcaacg agcattgatg tagccatttt
    23701 taaccgtaat gtctgccccc atggctctta atgcttttaa atgcaggtca actggtcttg
    23761 ttccaatggc gcatccaccg ggcaaagaaa catctgcctt accgaagcgc gccagcattg
    23821 ggcctaatac caaaatggag gcacgcattg ttttgaccag atcgtaagga gcaacaaact
    23881 cattgacttg actggagtca acttgaacat tcatcttttc atcaacaatt aaatgagctc
    23941 ccaattgtcc taacagttcc atcatggtag tgatgtcttt aagatgaggt acattagata
    24001 ttgtgacgtg atcactggct aatagactgg cagccatgat tggaagagca gcgttttttg
    24061 ctcccgaaat gaccacttca ccatgtaatg ctttaccacc attaatcaat aatttatcca
    24121 ttttgcttcc cccattcttc ttttgtccat gtcttcatac tgatagcatg aagccttccg
    24181 gtggtaataa agtctttaag ctgggcataa acccattgct gtcttgctac tttagacttc
    24241 ttaagaaata tatcggtcac tacagttacc tgatattgat aaccatcgcc ctctaccttg
    24301 acataaaata catcgtcaat ttcattaagt ctttgttcaa tttcttcatt acttaacata
    24361 aacttagctc actaattaaa ataaatacaa attttttata cttttattga taaaaaataa
    24421 tggacatgac tgaatcatta tcaaaaaaat ccgtgtttac accatcattc tctaaagatt
    24481 tagactgttt caccaaccac aacattacct caaaattcat gggaatttat caattcagga
    24541 ggacaactag agtctgttaa tttttctctg gcttgaaacc caaaactttg atttttaagc
    24601 accgtagcag aaatatcaaa ccattattaa gcaacggaac acagaaaacc aacgctttaa
    24661 agccaagaca gtagaaatgt cgatagattc tagcgctcta aagggataag ctctatggaa
    24721 taataaatat ttatgactca gaattgtttc aagatctttg taaaatagtc ttcactccac
    24781 agaattcagc cagagattgt gtttcaggag atattcctat tacatcaaaa accttattat
    24841 tttgtttaca taattttcta gcttcaataa ttaaagcaag tccagcacta tcgcaatgag
    24901 tcacctcgct taaatcaagg caaaatctct ctctggtatc ttccattaaa gccttataca
    24961 gcttgtcacg gaccgatacc actgatttga atgtaagctc agcgccaggt ttaaagttat
    25021 taatttccat gctcaggaag ccttcttaat ctgtttttgt tgcatttcct taatcacttg
    25081 actaatgctg gaatgttgca gtgcctgtgc aaattgcgat ctaaaacttt gcaacaaact
    25141 aacaccttca acgcttatat cataaatttt ccatttccca ttcttggaca caagactata
    25201 acttaatggt atattttgcc ctgcagaacg cacaatcacg ctatttacac gaataaaccg
    25261 actatttaaa gagccgcgta aaggtaaaaa ctgcacagtt tcatcggaat attgggccaa
    25321 tgggctagaa tatgtacgaa tcaccaagcg agtaaatgct tttgagaatt caaccctttc
    25381 cgcttcagta gctttactcc aggcttgtct tcccaagact gaacgagaca taccagcaac
    25441 gtcaacgttg ggtaaaagat tctcctcgac tgctttgtaa ataatctcag gattactttt
    25501 taagcgcgct ttattttctt tgagaataga aataatttga ttagctgcac gttctaacat
    25561 agggatagga gaggtctgag caaccattgc ttgagacaac gtcatcaaca caactaataa
    25621 aattgtttta aaaactctca tggttcacct atttttttat attaaataat aattggccta
    25681 taagattttc aaggataatt gcttcctgag tcttcgcaat ggtatcccca tcgcgtaaat
    25741 agggatgatc tgtgtcaccg tcctcgtcaa aaccgggaac gatactaata taattcgaac
    25801 caagcaagcc ttcggtcaaa attcgtgcag aagcatcctc ataaggaatc tttttatcac
    25861 cattcaaagt catagtcact cttgcattga gctcacctgg ctgcaactca attcgactga
    25921 cctcaccaat acggacacct gcaacggtca ctggagctcg aacttttaaa ccaccgatat
    25981 cagtaaaatc tgcagtgaca cgataagttt tatcagacat aaaatcagta atgttactca
    26041 ctttcatcgc cattactaat aatgctagca gcccaagcaa cataaatatt cctacactaa
    26101 tgtctacata acgttgcttt ttcatttacc aatctccaat catcattgca gtcaacaaga
    26161 aatcaagacc taatacagca agcgatgagt atactacagt tttagttgta gcatgactaa
    26221 tgccttcagc tgttggcaca cactcaaaac cttggtatac agaaatccaa gtaaccacaa
    26281 atgcgaaaac caagctttta ataatcccgc ttaagatatc tatacgaaaa tttacagcag
    26341 cttgcatatt agaccaaaaa ctccctgcat ccacgcctag ccagtaaaca ccaataaaat
    26401 aaccaccata tatagctact gcagaaaaaa tcaaagcaag tatcggtaac gctataaatc
    26461 ctgccacaaa acgcggataa accactcttc ctaatggatc cactcccatc atatccatac
    26521 tggataactg ctctgttgcc ttcattaaac caatttcagc tgtaagggcc gacccggcac
    26581 gccctgcaaa caacagagcg ctaatcacag gtcctaattc tctggcaacg cttaaagcca
    26641 ataattgacc taattgactt gaagcaccaa atttttgtaa cgtattatac ccctgaagtc
    26701 ctacaaccat accaataaac aaggctgaaa ccacaataat taaacaagac aatacaccga
    26761 taaaataaag ctgataacgt aataatggcc ataaccttaa aacattaggc tttctgaaca
    26821 acatggaaaa taaaaaaagc cctgaactac caataccttg taaggcttgt acacctcgat
    26881 tacccagacg cgcgattgta tcaagcattt aataactcct ctgtatacgg ttttgccgga
    26941 taatggaaag gcactacccc atccgcttct ccatgcataa actgtcttat ctgaggctca
    27001 atagatcgct ttaattcatc cggagtacct tgacctataa ttttaccccc tgatattaag
    27061 tagatataat ctgcaattga acaagtttct tcaacatcat gagatacgat aatagtggtg
    27121 gtatgcaata attgattcaa tcgttgtatt aatctgacca atacccccat ggaaattgga
    27181 tcttgaccgg taaaaggctc atcatacatc attaatacag gatcaagagc aatggttctg
    27241 gctaatgcaa ccctcctgca cattccccct gataattgag cgggcattag atgggcggct
    27301 ccacgcaaac caaccgcttc gagcttcatt aatacaatgt ttcttatcat ggatgcgttt
    27361 aattcagtat gttctcttaa tggaaaggca acattttcaa atactgaaag gtgtgtaaat
    27421 aaggcactac tttgaaaaag caatcccatg tttcgcctgg tttcataaag tgatttgcgg
    27481 gataattgat gaatattctg accattaaca taaatttggc cactatcagg cctcaattgc
    27541 gcaccaatta accgcagtaa agtagtcttt ccagaaccac tgggtcccat aatagcggta
    27601 atcttgcctt gtttaactac catatcaatg ccatcaaaaa tgcagcgatc atcaaggcta
    27661 aagaatagat tttttatttc aacccaatta tcaggcatgc tttattatcc acttattatt
    27721 ttaacgggta atgatgtcag tttgatatct atcaaagaca ggtaatgctt tattatatct
    27781 ttaattaaga agtatatact ggataaataa aaatgtgttt aataattgcg tttccattaa
    27841 aaaaaaactt tttaaaagtt tttttacaat accaatttct caaaagttaa tcagaaaatt
    27901 taaataaaag gta
//
LOCUS       DATNSN010000026        26159 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.026, whole genome shotgun sequence.
ACCESSION   DATNSN010000026 DATNSN010000000
VERSION     DATNSN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26159
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.026"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..303
                     /gene="tuf"
                     /locus_tag="VAH76_000928"
     CDS             <1..303
                     /gene="tuf"
                     /locus_tag="VAH76_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO1385575.1"
                     /translation="KPGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDV
                     TGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE"
     gene            309..626
                     /gene="rpsJ"
                     /locus_tag="VAH76_000929"
     CDS             309..626
                     /gene="rpsJ"
                     /locus_tag="VAH76_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO1385576.1"
                     /translation="MSSNQNIKIRLKSFDHRLIDLSTREIVDTAKRTGAQIRGPIPLP
                     IRKEKFTVLTSPHVNKDARDQYELRTHKRLVVIVHPTEKTVDALMKLDLAAGVDVQIS
                     LDD"
     gene            661..1311
                     /gene="rplC"
                     /locus_tag="VAH76_000930"
     CDS             661..1311
                     /gene="rplC"
                     /locus_tag="VAH76_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO1385577.1"
                     /translation="MMIGLLGRKIGMTRVFTPEGVSVPVSVVEVQPNRVSQVKTAAND
                     GYSAVQLTGGTKKSSKVSKPVAGHFAKAQIDAGDMQVEFRIDSEDAFTPGQVISVADV
                     FTAGQYVDVSGLTKGKGFAGTVKRHNFRTQDASHGNSRSHRVPGSIGQNQTPGRVFKG
                     KKMAGHMGNARCTIQSLELVKVDSERNLLLIKGAIPGAPGSRVEIKPAVKKQARGE"
     gene            1311..1916
                     /gene="rplD"
                     /locus_tag="VAH76_000931"
     CDS             1311..1916
                     /gene="rplD"
                     /locus_tag="VAH76_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO1385578.1"
                     /translation="MEITTIDTKSKLKLNKEIFAYTYNEGLVHQAVVTFMNNARSGNS
                     AQKTRSEVSGGGKKPWNQKGTGRARAGTIRSPLWRSGGVTFASKKRDYSQKLNKKMYK
                     RALRSIISELCRTGNLVVVSDFQCDNHKTKDFLKKMNQMEISNALIIMSEVGENEYLG
                     SRNLIDYDICDVTTIDPVSLLRFEKVVVTEAAIKKIEEQLQ"
     gene            1913..2209
                     /gene="rplW"
                     /locus_tag="VAH76_000932"
     CDS             1913..2209
                     /gene="rplW"
                     /locus_tag="VAH76_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO1385579.1"
                     /translation="MNAERLMMVLREPHTSEKATVMADKFKQFTFKVLKNATKTEIKL
                     AVEHIFNVKVKSVSVVNVKGKSKRFKQTSGKRSDWKKAFVSLHADQDIDFTVTE"
     gene            2221..3048
                     /gene="rplB"
                     /locus_tag="VAH76_000933"
     CDS             2221..3048
                     /gene="rplB"
                     /locus_tag="VAH76_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO1385580.1"
                     /translation="MALLKSKPTSPGKRGEIRVVHHDIYKGKPHAALVEKLKKTGGRN
                     NQGRITVRHIGGGQRQKYRIIDFKRNKDGILGRVERLEYDPNRTALIALISYKDGEKR
                     YIIAPSNLEVGATIQSGADSPISVGNCLPLKNIPVGTTIHCVEMKPGKGAQMLRSAGC
                     SGQLVAKEGVYATLRLRSGEMRKIHVLCRAVIGEVSNSEHNLRALGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGLPTKGYKTRSNKRTDTFIVRGRKKK"
     gene            3067..3345
                     /gene="rpsS"
                     /locus_tag="VAH76_000934"
     CDS             3067..3345
                     /gene="rpsS"
                     /locus_tag="VAH76_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO1385581.1"
                     /translation="MARSIRKGPFIDHHLISKVEAAIESKSKKPIKTWSRRSTIVPEM
                     IDLTIAVHNGKDHVPVFITDNMVGHKLGEFAMTRTFKGHSGDRKAKGK"
     gene            3355..3690
                     /gene="rplV"
                     /locus_tag="VAH76_000935"
     CDS             3355..3690
                     /gene="rplV"
                     /locus_tag="VAH76_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO1385582.1"
                     /translation="MEVTAKLKGAPLSAQKGRLVADMIRNMNVSGALDVLKFTPKKGA
                     KLMLKLLESAIANAENNNGADIDDLKVGMVCVDEATTLKRISPRAKGRANRICKRTCH
                     ITIKVSDEE"
     gene            3693..4349
                     /gene="rpsC"
                     /locus_tag="VAH76_000936"
     CDS             3693..4349
                     /gene="rpsC"
                     /locus_tag="VAH76_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO1385583.1"
                     /translation="MGQKVNPIGIRLGIIKDWNSKWFAGKRYAEFLIQDIKLRNDLKK
                     KLMAAAVSKILIERPANNAVVTILTARPGVIIGKKGGGIETLRKEISDNLGVPVHLNI
                     EEVKKPELDATLVAEGIAQQLEQRVMFRRAMKRAVTSALKAGAKGIKICVSGRLGGAE
                     IARSEWYREGRVPLHTFRADIDYGTAESKTTYGIIGVKVWIFKGEILPQKKRSTESAQ
                     "
     gene            4366..4779
                     /gene="rplP"
                     /locus_tag="VAH76_000937"
     CDS             4366..4779
                     /gene="rplP"
                     /locus_tag="VAH76_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO1385584.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGSKISFGEFGLKAVERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVAQIQPGKV
                     LFEMEGVSKELAMEAFDLAKAKLPFKVMFEERTVM"
     gene            4779..4973
                     /gene="rpmC"
                     /locus_tag="VAH76_000938"
     CDS             4779..4973
                     /gene="rpmC"
                     /locus_tag="VAH76_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO1385585.1"
                     /translation="MKKIDELRNMSVEELQNELLSLRKDQFNLRMKKASGSLDKTHLI
                     TMVRKSVAKVKTILTEKAGK"
     gene            4975..5229
                     /gene="rpsQ"
                     /locus_tag="VAH76_000939"
     CDS             4975..5229
                     /gene="rpsQ"
                     /locus_tag="VAH76_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO1385586.1"
                     /translation="MSTNSESNARTMIGKVVSDKMDKTIVVMIERTVKHPKYGKIMKR
                     RTKLHAHDENQVCRVGNTVKIRESRPLSKTKSWVLVEVIS"
     gene            5318..5683
                     /gene="rplN"
                     /locus_tag="VAH76_000940"
     CDS             5318..5683
                     /gene="rplN"
                     /locus_tag="VAH76_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO1385587.1"
                     /translation="MIQMQTVLEVADNSGARKVMCIKVLGGSHRRYARVGDVIKVSVK
                     DAIPRSKVKKGAVMRAVVVRTAQGVRRDDGSLIRFDDNAAVLLNNQNEPIGTRIFGPV
                     TRELRERFMKIISLAAEVL"
     gene            5696..6025
                     /gene="rplX"
                     /locus_tag="VAH76_000941"
     CDS             5696..6025
                     /gene="rplX"
                     /locus_tag="VAH76_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO1385588.1"
                     /translation="MKRIKSGDEVIVIAGKSKGHIGKVLRVIDDAVVVEGGNLIKKHI
                     KPNPQKPENKGGIITREAPLHVSNVAHYNPVTKKADKVGFKYLESNGVSKKVRYYKSN
                     NEIIDRI"
     gene            6041..6592
                     /gene="rplE"
                     /locus_tag="VAH76_000942"
     CDS             6041..6592
                     /gene="rplE"
                     /locus_tag="VAH76_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO1385589.1"
                     /translation="MARLKEFYKKDVVTMMMKRFNYSSVMEVPRILKITLNMGVGEAV
                     GDKKVMNHAIEDMTLISGQKPVVTKARKSIAGFKIREGWPIGCKVTLRRERMYEFLDR
                     LISITLPRVRDFRGLNPKSFDGTGNYSMGIHEQIVFPEIDYDKTDGIRGLDICITTSA
                     KTNEEAKALLEAFNLPLKDKDRK"
     gene            6605..6907
                     /gene="rpsN"
                     /locus_tag="VAH76_000943"
     CDS             6605..6907
                     /gene="rpsN"
                     /locus_tag="VAH76_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO1385590.1"
                     /translation="MAKKSMLMRESKRAKLVEKYRQRRNELKQLIKSSDDFQVIMESQ
                     AKLAKLPVNSNPVRYVTRCKQCGRPHAVYRKFNLCRICLRQQLMVGNIPGGRKSSW"
     gene            6936..7325
                     /gene="rpsH"
                     /locus_tag="VAH76_000944"
     CDS             6936..7325
                     /gene="rpsH"
                     /locus_tag="VAH76_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO1385591.1"
                     /translation="MHDPIADMLTRIRNGQQAKHQQVTLVSSKLKEEIARVLKEEGYI
                     QDFFTETLPNGLKSITLKLKYYHGRPVIEFIKRISRPGLRVYKSYKDLHSIPGFGVAI
                     LSTSKGIMTHVSAKVKGVGGEVICEVA"
     gene            7343..7882
                     /gene="rplF"
                     /locus_tag="VAH76_000945"
     CDS             7343..7882
                     /gene="rplF"
                     /locus_tag="VAH76_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO1385592.1"
                     /translation="MSRVAKAPVIHSANVEVTFVDGVITVKGPKGILTQKINKLVNIQ
                     HSKESNKLEFSPASNDPMGWAQAGTARALVRNMVQGVTEGYTVTLELVGVGYRAQSKD
                     KSISLSLGYSHSIEYDLPKGVTVETPNNTTILLKGVDKQVLGQIASEIRAFRPPEPYK
                     GKGVKYAGEQIVRKEAKKK"
     gene            7893..8252
                     /gene="rplR"
                     /locus_tag="VAH76_000946"
     CDS             7893..8252
                     /gene="rplR"
                     /locus_tag="VAH76_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO1385593.1"
                     /translation="MNKQNARHRRGLKAKALIRKTGRSRLVVYRSGVHIYSQIVKSDQ
                     LGDKVLVASSTIDKELRSSLTGKSKVEQASLVGQLLGKRAKAAGITQVAFDRAGYKYH
                     GRVKALAEGAREAGLDF"
     gene            8262..8768
                     /gene="rpsE"
                     /locus_tag="VAH76_000947"
     CDS             8262..8768
                     /gene="rpsE"
                     /locus_tag="VAH76_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO1385594.1"
                     /translation="MSFDELPKSDGYQEKLVSVTRTAKVVKGGRVFGFAVLVVVGDGK
                     GKVGFGRGKAREVPIAIQKAMDQAKKNMVYIPLSGTTIFHEITWNYGASKVFMKPASE
                     GTGIIAGGAMRAVLEVLGVQNILAKSIGSTNPSNIVRATIGALTHIGTPDYVAAKRGK
                     TVEEVMAG"
     gene            8771..8956
                     /gene="rpmD"
                     /locus_tag="VAH76_000948"
     CDS             8771..8956
                     /gene="rpmD"
                     /locus_tag="VAH76_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO1385595.1"
                     /translation="MGKKIKITLVKSTIGRKPKHVAIAKQLGLGKTNSSVVHSDTPAI
                     RGLVNEINYLLLVEESA"
     gene            8956..9390
                     /gene="rplO"
                     /locus_tag="VAH76_000949"
     CDS             8956..9390
                     /gene="rplO"
                     /locus_tag="VAH76_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO1385596.1"
                     /translation="MNLNTLSPDPGSRPSRRRVGRGIGSGLGKTCGKGHKGQKSRAGG
                     YHKINFEGGQMPIQRRLPKMGFKSRVGRTIDEVSLGELAKLNDEVIDLVALRKAGLIN
                     NSIKDVKVILSGELTAAIKLKGLRVTKGARSAIESLGGSIEE"
     gene            9393..10721
                     /gene="secY"
                     /locus_tag="VAH76_000950"
     CDS             9393..10721
                     /gene="secY"
                     /locus_tag="VAH76_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO1385597.1"
                     /translation="MKNQKHNGSKPRGGLAELKSRLLFVVLGILVYRLGAHIPVPGLD
                     PAKLANFFSEQQNTIFGLFNMFSGGALSRVTVFAIGIMPYISASIIIQLFSVVSPKLE
                     QLKKEGESGRRKINQYTRYLTLLLSIFQSMGMARWLASQQIAMQPDFSFYFTAVVTLV
                     TGTMFLMWLGEQITEKGVGNGISLIIFSGIVSSMPNAIGSVLQQVKEGQMQALTLVII
                     AAVVIVVTGFVVFMERAQRRIRVNYAQRTQGRKVYAAQTSHLPLKINMSGVIPPIFAS
                     SIILLPATLAQFFSQTKGLGWLSDVGMALSPGQPLYLIVYAAAILFFAFFYAALVFNP
                     KDTADNLKKSGAYIPGIRPGEQTTKYIDSVMTRLTLVGAIYLVLVCLLPQILMYTWHV
                     PFYFGGTSLLIIVVVIMDFVAQVQAHLMTQQYDSLLKKANFRGTKLPGLL"
     gene            10738..10851
                     /gene="rpmJ"
                     /locus_tag="VAH76_000951"
     CDS             10738..10851
                     /gene="rpmJ"
                     /locus_tag="VAH76_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO1385598.1"
                     /translation="MKVRASVKRICRNCKIIKRSGTIRVICKDARHKQKQG"
     gene            10929..11285
                     /gene="rpsM"
                     /locus_tag="VAH76_000952"
     CDS             10929..11285
                     /gene="rpsM"
                     /locus_tag="VAH76_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO1385599.1"
                     /translation="MARIAGVNIPDHKHVVIALTAIYGIGKTTSLKLCKTVDIDPSVK
                     VSQLSDAQLESLRTEIAKITVEGDLRRVVTMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     KTNARTRKGRRKGTSS"
     gene            11309..11707
                     /gene="rpsK"
                     /locus_tag="VAH76_000953"
     CDS             11309..11707
                     /gene="rpsK"
                     /locus_tag="VAH76_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO1385600.1"
                     /translation="MAITKSKQQKTRKKVKRVVSDGIVHIHASFNNTIVTFTDRQGNA
                     LCWATSGGSGFRGSRKSTPYAAQVATERAAAVAKEYGMKSVAVFVHGPGPGRESTIRE
                     LITQDFKIVEITDVTGIPHNGCKPPKKRRV"
     gene            11724..12344
                     /gene="rpsD"
                     /locus_tag="VAH76_000954"
     CDS             11724..12344
                     /gene="rpsD"
                     /locus_tag="VAH76_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO1385601.1"
                     /translation="MARYLGPKCKLSRREGCDLLLKSGVRDHKSKCKSEKLPGQHGDK
                     KPRLNSYGIQLREKQKIRRLYGILEKQFRNYYKKAARQKGSTGENLMALLERRLDNVV
                     YRMGFASTRAEARQLVAHKAILVNDKVVNVPSFLVNPGDTVSVRQKAKNQGRIQAALA
                     LSEQRAPCDWITVDTGSFKGTFSTAPTLMDLSSDYNVNLVVELYSK"
     gene            12363..13355
                     /gene="rpoA"
                     /locus_tag="VAH76_000955"
     CDS             12363..13355
                     /gene="rpoA"
                     /locus_tag="VAH76_000955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO1385602.1"
                     /translation="MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALR
                     RILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQVTL
                     NKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRY
                     HEDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEA
                     IRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAEN
                     IHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE"
     gene            13374..13757
                     /gene="rplQ"
                     /locus_tag="VAH76_000956"
     CDS             13374..13757
                     /gene="rplQ"
                     /locus_tag="VAH76_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO1385603.1"
                     /translation="MRHRNSGRSFSRTSSHRKAMFSNMCCSLIEHELIRTTLPKAKDL
                     RRYIEPLITVSKSDSVASRRRAFDILRSKSAVGKLFTDLGPRFAKRPGGYIRIIKCGY
                     RDGDNAPMAIVELMDRPVSSDDTEE"
     gene            complement(13824..14303)
                     /gene="ssb"
                     /locus_tag="VAH76_000957"
     CDS             complement(13824..14303)
                     /gene="ssb"
                     /locus_tag="VAH76_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO1385604.1"
                     /translation="MARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSETWKDKT
                     TGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEGSLRTRKWQDQQGQDRYTTEIV
                     ASDIQMLDSKGNGATNYDDMPSFQGTSTPQQASTKNQATPTSTAQDAFDELDDDIPF"
     gene            complement(14387..15754)
                     /locus_tag="VAH76_000958"
     CDS             complement(14387..15754)
                     /locus_tag="VAH76_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385605.1"
                     /translation="MKYSWNKTVFPIAAIFSFRLLGLFLLIPVFTLYATNLKGATPTL
                     IGLALGSYGLAQGLLQIPFGMLSDKLGRKPMIAIGLLLFAGGSLLGAVTHSIAGMITA
                     RILQGAGAIGSVLIALLADLTLDEQRTKAMAIVGITIGTSFSLAMIISPALTHYYGLS
                     GVFYLTAALAVLGIMILYLIIPNPSKEQFHADSEPSLSLIKPVVTDQHLQRLNFGIFC
                     QHFILTATFFAIPMLLKYQIETGHLHQQWHFYLPLMLFSFILMVPFIIISERKKRLKT
                     VFVISVLLTTFTQGLLVFTSQNWYWFCILMFVYFVSFNVLEATLPSLVSKLASPGSKG
                     TAMGIYSTSQFLGIFFGGSLAGILYQWYDNQAIFLINMILSCLWLIISLSMKSNVYFS
                     TLILPFTANNEESKLVVNQLLKVTGIKEVILSKDENTLYVRVDNEEYSTGSAEKILHQ
                     STIYQ"
     gene            15916..16674
                     /gene="fabL"
                     /locus_tag="VAH76_000959"
     CDS             15916..16674
                     /gene="fabL"
                     /locus_tag="VAH76_000959"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HEO1385606.1"
                     /translation="MSSLVFSGKVGLITGGARGIGKATALKLAHSGCDIAIVYYNSSD
                     EAQSLVNEITSMGRKAIALQADVADCQSVKEMFSQFREHFKHLNFLVSNAASGVLKPA
                     LKMSTKHWRWCLETNALALNHLVTEGLDLMPKNSRVIALSSLGASRAIPNYAFIGASK
                     AALEALVRSLSLELASYGITVNTVSAGVVDTDALKHFPNREQLLDEYQAHALADRALT
                     PEDVANAIYLLCLPEAAMINAHTLYVDAGYSQVG"
     gene            16745..17158
                     /locus_tag="VAH76_000960"
     CDS             16745..17158
                     /locus_tag="VAH76_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1385607.1"
                     /translation="MNVAEVYPKVREIIADVLVIDEEEISLNSSLIEDLGAESIDFLD
                     LVFQLEKEFKIKIPRGQLEKNARGDLAEDEFEKGGILTTSGLQALKNYLSEVPADRFK
                     ENMKVNEIPVLFTVETFCKLVISAIAQQQATETVA"
     gene            17158..18054
                     /locus_tag="VAH76_000961"
     CDS             17158..18054
                     /locus_tag="VAH76_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEO1385608.1"
                     /translation="MRFLFVDRIVQLSPGEWIKGIKHVTRDDAYLTTDDQGRLCFMPS
                     LIGETLGQLAAWNVMQHNHFTSRPVAGVVSSARLYRPAYVGETILLESNIDSLDEKAV
                     QYHSIAKVGNDIIFTIDGALGPMLPMTEFISTEVIRQQFAEIYRPGEWSSVLALSSPV
                     LIEENCSDCGMINSSMQFDRVLASEPGISLIAEKRITRSASYFPDHFPNRPVLPMTVL
                     LECKLNLAKEFIARAKYDRAYRISELRKIKMNEFVHPGDVVVCQVKVKQQMDDELILS
                     YRCEVDGKRVCVVDIVLIPKGK"
     gene            18059..19351
                     /locus_tag="VAH76_000962"
     CDS             18059..19351
                     /locus_tag="VAH76_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO1385609.1"
                     /translation="MKRRVAITGLGVVSPLGNDVSTTWNNLIAGYSGIDSIKQFDASA
                     FPTTIAAEVKNFSPDTSISGKHTRFIMSFTHFALEAARQAFEDASIFPTKQTSDRWGI
                     VTGSGMMTAEFEYLTRFQQTCAADGEIDWGKLQSNSRDFYNLVDFGKTTSNSGLSLLI
                     QQYGITGYASSVHTACASGGQALGLAMQVIRRGEADFMLAGGFDSMINPLGLSSFCLL
                     GALSTYNETPQTASRPFDATRNGFVLGEGAAFLILEEWSKAKARGAKIYAELAGEGNS
                     LSSYRITDSHPNGDGAIQAIKRALEDAGVQPRDVDYINAHGTSTKMNDLSETNAIKVV
                     FGDRIANLPVSSTKSQTGHLIAAAGALEAVLSVKSIEQAQVPKTANLKTPDPECDLDY
                     VVDGPREKSLGVVLSNSFGFGGSNSCLLFKHPEFEEAK"
     gene            19352..20629
                     /locus_tag="VAH76_000963"
     CDS             19352..20629
                     /locus_tag="VAH76_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEO1385610.1"
                     /translation="MSQANRVFITGRSALTASGATADETWNAIISGQCGIDEINFWEL
                     SEWSHRLGGEIKDFQPSKMLPDRKLIKVISRQDIMGINAAVQAVEHSKMIAYRDSLAD
                     ADLFNEQTAIYVGSPGNKYFQQYDFIPLLAKTHGDMRSFANQLFDEVHPMWLLRILPN
                     NVLAYTGITYGFKGPNHNVTNHAVGGTQAILEAYHAIRSGQAERAVVVAYDIGTEPQG
                     LFYYEKLGVVSNRHLKPFDKEHDGTILAEGAAALVLESEASVKSRAATCYGEIVGGLS
                     ASEATGLFSIEADGQHLASLMRQTLSTSGISSEEIGLIVTHGNGNSKSDDSEALAIKS
                     VFKQEEVPATAFKWSMGHTLCASGVLDAVLTTYALETKCVPGIANFQQIAPTCEGLSV
                     SREHRETISNSYAMMINRGFASMNVCLVIKACE"
     gene            20622..21467
                     /locus_tag="VAH76_000964"
     CDS             20622..21467
                     /locus_tag="VAH76_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEO1385611.1"
                     /translation="MNNLAQQIEALPITLAGRFIYRFLPYRRRLIFSNISQVYNDQLN
                     ESQKKRLAKAYYSHLAKSLKEALQLRFMSEKKLRAQVEVIGHEKMLAVVAQKKGVLVV
                     TGHFGNWEFAPLGGVLNFKEFKGQFHFIRRTLRFKFIERIMFKNYYQAGLNVIPKKNS
                     LEQVCVALEQNHAVIFVLDQHASLVNRDGIAVEFFGKKAGTYRSLATISRHTGIPVIP
                     AASYRLPNGKHVLEFHDPIPWKDYDTTQESLYRNTLAYNQALEKIILAHPEQWNWMHK
                     RWKLS"
     gene            complement(21576..21881)
                     /locus_tag="VAH76_000965"
     CDS             complement(21576..21881)
                     /locus_tag="VAH76_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0364 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEO1385612.1"
                     /translation="MDERRKYFRLKNHGEINASLDNNPIEIVEISSNGAVVVKQKTDI
                     PKEGVLKLQIHNFIMELCYEVIRAEDNNIVLHFTKEDETNKLFLVLKRLRDERKNKT"
     gene            complement(22057..24747)
                     /locus_tag="VAH76_000966"
     CDS             complement(22057..24747)
                     /locus_tag="VAH76_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0365 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385613.1"
                     /translation="MVDFDKPVSKNDILASLLLIADLNRPSLNQTLSWEQALKSFLSL
                     NDPLIKTEIFIKWLSQLNPDQLQSSPEIVLILKHIVEQKLLPYTLKKILIQRENFTTS
                     QINWLCNHCLIVAPDQTTLPDIIKLGSWSWLENHMKRNELLNFDLLKIAILNKDYLAS
                     INNSKESQLDFIYALNANKFNYQQILELIALAQDSQIKSLLITCLLSKKEYLDNLKEK
                     SFLAHLNQSEFHSPPRLNALVHQLNLSCLTVAQIELLQPEACVTLLCSIPHFHQLSEK
                     QVEKILLKYPKPDMIAYWLNHYSSRPNAHFILAHLMNLVDVHVVDELNKMDSTKKETI
                     IMNMVEHLELFDPTHPLLINQNEEKRLITAIQRYLYGQHHDAYVTYIKLLVYKLLNRN
                     YQFSPQAMQLLICLNNLEEFKELNNKIAYLTNYYLRASAQSGDSSLFYNDSRINITSM
                     TQLVQLAPKIPTKSKEKSLLSSLFCGIKISESSVMTPTNDIPEHPLINKLIKSKRTVK
                     SIDYFLMHYKGDKQVLSKIINDYLSYYVQEGCTEIHRRSIYHLIVLMSLSDISASVRE
                     AIYTAFLNYPELHDEQINFSLFIYDAKRTIRHFGAQASEDSYARVIDLCEGALKKLNP
                     NKHKEIISIATQALTEAKIELSFGENTGFIAQLISRLKRCWYYGWTGFFSPNLPAFVA
                     AIDSPIKQSEIKASPLLQQRKYFTTKLETNLSIILDDIKSGYTQQKLNELIEALSMFS
                     LQSNPKNELKIRQKLHRLFHFLLLESKNDLTLDAWLIKNQQLLIANRFRLLEMQLMKG
                     ARNEVESLLKEFDEDSTHFSPITKEFTHSLPEQNPYKCESVSSNQTPGATILDSATEL
                     ATSLKSTLGNLGVFFSNNIMSAIYPNNKSTPQSTSTMISP"
     gene            complement(25060..25893)
                     /gene="dapF"
                     /locus_tag="VAH76_000967"
     CDS             complement(25060..25893)
                     /gene="dapF"
                     /locus_tag="VAH76_000967"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEO1385614.1"
                     /translation="MGIKFTKMHGLGNDFIVLDGVNQSIQLTVKQIQKLANRHTGIGF
                     DQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATK
                     TTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGN
                     PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETI
                     ACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLL
                     S"
     gene            complement(25903..26031)
                     /locus_tag="VAH76_000968"
     CDS             complement(25903..26031)
                     /locus_tag="VAH76_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO1385615.1"
                     /translation="MRHIYFALLLIVVTLTSLCLSACGQKGPLYLPEQEKKATTNQ"
BASE COUNT         8355 a         4266 c         5698 g         7840 t
ORIGIN      
        1 aagccaggaa ctatcaagcc acacaccaag tttgaagcag aagtgtatgt attatccaaa
       61 gaagaaggcg gacgtcacac tccattcttt aacggatacc gtccacaatt ctatttcaga
      121 accactgacg tgacaggtac ttgtgacttg ccatcaggag ttgaaatggt aatgcctgga
      181 gataatgtgc aattagttgt tagcttgcat gctccgattg cgatggatga aggtttaaga
      241 ttcgcaatta gagagggtgg ccgaactgtt ggcgccggtg tagtcgctaa aataatcgag
      301 taaaaaaaat gagcagcaat caaaatatta aaataagatt aaaatccttt gatcatcggt
      361 tgattgatct atcaacacga gaaatcgttg atactgcgaa aagaactggc gcacaaatac
      421 gtggaccaat tcctttgcct atcagaaaag aaaaatttac tgtattgacc tcaccgcatg
      481 taaataaaga tgcaagagat caatatgaat tacgtactca taaacgcctg gtcgttattg
      541 ttcatccaac tgaaaaaact gtagatgctt taatgaaatt ggatttggct gccggggttg
      601 atgttcagat aagccttgat gactaaatat aaaggcaaag gatattaaag aggtgttaca
      661 atgatgatcg gtttattagg ccgtaagatc ggtatgacgc gtgtcttcac accagaagga
      721 gtttctgttc ctgtttctgt tgttgaggtt cagccgaatc gcgtttctca agtaaaaaca
      781 gccgcgaatg atggttattc agctgtacaa ttaactggtg gtacaaagaa atctagcaaa
      841 gtgagtaagc ctgtggctgg gcattttgct aaagcacaaa tcgatgctgg tgatatgcaa
      901 gtagaatttc gtatagattc agaggatgca tttacaccgg gtcaagtcat atctgttgca
      961 gatgtattta ctgccggaca atatgttgat gtatcgggtc tgactaaagg taaaggtttc
     1021 gcaggaactg taaaacgaca taactttaga actcaagatg catcgcatgg taactcaaga
     1081 tcacatagag tacctggttc tataggacaa aaccaaaccc ctggtcgtgt ttttaaaggg
     1141 aaaaaaatgg ccggtcacat gggtaatgct cgatgtacaa ttcaaagtct ggaattggta
     1201 aaagttgact ctgaaagaaa tttattgctc attaaaggtg caattcctgg tgctccaggt
     1261 tctagagtag aaattaagcc tgcagtcaaa aagcaagcac gaggtgagtg atggaaatta
     1321 ctacaataga tacaaagtca aaattaaagc taaataaaga gatatttgct tatacataca
     1381 acgaaggctt ggttcatcaa gcagtagtta ctttcatgaa caatgctcga agcggtaata
     1441 gcgctcagaa aactcgttct gaagttagtg gtggtggtaa aaagccctgg aaccaaaaag
     1501 gaacaggtcg tgcgcgagca ggaaccattc gcagcccatt atggagaagt ggtggagtaa
     1561 catttgcatc taaaaaaaga gattattcac aaaagcttaa taaaaaaatg tacaaacgtg
     1621 cattacgtag tataatctcc gaactttgcc gcactggcaa cttggtggtt gttagtgatt
     1681 tccaatgtga taatcacaag accaaggatt tcctcaaaaa aatgaatcag atggaaataa
     1741 gtaatgctct gattataatg agtgaagttg gtgaaaatga ataccttggt tcaagaaacc
     1801 tgattgatta tgatatctgt gatgttacaa ctatagatcc tgtttcctta ctacgatttg
     1861 aaaaagttgt tgttacagaa gctgcaatta aaaaaattga ggaacagtta caatgaacgc
     1921 tgagagattg atgatggttc ttagagaacc acatacttct gaaaaggcaa ctgtcatggc
     1981 cgataagttt aaacagttca ctttcaaagt actgaaaaat gcgactaaga ctgaaattaa
     2041 attagcagtt gaacatatat ttaatgtaaa agttaaaagt gtatctgttg tcaatgtgaa
     2101 gggtaaatct aaacgcttca aacaaaccag tgggaagcga agtgactgga agaaggcatt
     2161 tgtctctctt catgcagacc aagatattga ctttacagtt actgaataag gcagataaag
     2221 atggcactat taaaatctaa accgacttca cccggaaaac gaggtgaaat acgtgttgta
     2281 catcacgata tctataaagg aaagccacat gctgcattag ttgaaaaact aaaaaaaaca
     2341 ggtggaagaa ataaccaagg tcgtataact gttagacata ttggtggtgg acaacgtcaa
     2401 aaatatcgaa ttatagactt taaacgcaat aaggatggta tattaggtcg tgttgagaga
     2461 ttggaatatg atccaaatag aactgcgcta attgcactga tatcctataa agatggtgaa
     2521 aagcgctata ttatagcacc atcaaatctg gaagttggag ctactattca aagtggagct
     2581 gattcaccaa tcagtgtagg gaattgtctg ccactaaaaa atatacctgt aggtacaaca
     2641 atacattgtg ttgaaatgaa gcctggtaaa ggtgcgcaaa tgctcagaag tgctggttgc
     2701 agtggtcaac ttgttgcaaa agaaggtgtt tatgcaactt taagattacg ttcaggagag
     2761 atgcgcaaga tacatgtttt atgccgagct gtaattggtg aagtaagtaa tagcgaacac
     2821 aacctaagag cattgggtaa agctggtgcc aaacgatgga gagggatacg tcccactgtt
     2881 cgtggggtcg ccatgaaccc agttgatcat ccacatggtg gtggtgaagg acgaacatca
     2941 ggtggacgtc atcctgtatc tccatgggga ttgccaacaa aaggctataa aactagaagc
     3001 aataagcgta ctgacacatt tattgtcaga ggacgtaaga agaaataatt aatatgagga
     3061 tatcaagtgg ctcgttcaat tagaaaaggt ccatttattg accatcattt gatcagcaag
     3121 gtagaagctg caatcgaatc aaaatcaaaa aagccaatta aaacttggtc tagacgttct
     3181 acaatcgttc ctgaaatgat tgatttaaca attgctgtgc ataacggcaa agaccacgtt
     3241 cctgtattta ttacagacaa tatggttggt cataaattag gtgagtttgc catgactcgt
     3301 acattcaagg gccactctgg ggacagaaag gctaaaggta agtaagagga tatgatggaa
     3361 gtaacagcta aattaaaagg cgctccttta tccgctcaaa agggtagatt agtagccgat
     3421 atgatacgta atatgaatgt gtctggtgcg cttgatgtcc tcaagtttac accaaaaaag
     3481 ggtgctaaat taatgcttaa attattagag tcagctatag caaatgctga aaataataat
     3541 ggagctgata ttgatgatct gaaagtaggc atggtatgcg ttgatgaagc aacaacttta
     3601 aaacgtatta gtcccagagc taagggtaga gcaaatcgaa tttgcaaacg tacctgccat
     3661 ataactatta aagtgtctga cgaggaatag caatgggaca aaaagtaaac ccaataggta
     3721 tacgccttgg aataattaaa gattggaact caaaatggtt tgcaggaaag cgatatgctg
     3781 agtttttaat tcaggatata aaactacgta acgatcttaa aaagaaattg atggctgcag
     3841 ctgttagcaa gatacttatt gagcgaccag cgaataatgc tgttgtgact atcttaacag
     3901 caagaccagg tgttattatc ggcaaaaaag gcggaggaat tgaaacactt cgtaaagaaa
     3961 tttccgataa cctgggtgtg ccagttcatt tgaatattga agaagtgaaa aaacctgagt
     4021 tggatgctac tttagtggct gaaggtattg ctcagcagtt agagcaacga gtcatgttca
     4081 gacgagctat gaaacgtgct gttacctctg ctctgaaagc tggagcaaag ggtattaaaa
     4141 tttgtgtaag tggccgattg ggtggtgctg aaattgcaag aagtgaatgg tacagagaag
     4201 gacgagtgcc tttacatact tttagagctg atattgatta tggtaccgct gaatccaaga
     4261 ctacctacgg aattattggc gtaaaagtgt ggatctttaa gggtgaaatt ctcccgcaaa
     4321 agaaaagatc aactgaaagc gcccagtgag tgaggattag aaattatgtt acaaccaaag
     4381 cgtactaagt accgaaaaca aatgaagggt cgcaatagag gtttggctct tcgtggtagt
     4441 aaaattagtt tcggtgagtt tggtctaaag gctgtagagc gtggacgttt aactgctaga
     4501 caaatcgaag cagcgcgaag ggctatgaca cgacatatta agcgtggtgg taaaatttgg
     4561 atcagagtat ttccagataa accaatcaca caaaaaccac ttgaagtaag acaaggtaag
     4621 ggtaaaggaa gtgttgagta ttgggtagcc caaatacaac caggtaaggt tttatttgaa
     4681 atggaaggtg ttagcaaaga gcttgcaatg gaagcatttg accttgcaaa agcaaaactt
     4741 cctttcaaag tgatgtttga agagcggacg gtaatgtaat gaaaaaaata gatgaattgc
     4801 gcaatatgtc agttgaggaa ttgcaaaatg aattgctttc attacgtaaa gatcaattta
     4861 atttacgaat gaagaaagca agtggctcac tagataaaac acatctgatc actatggtgc
     4921 gtaagtcagt ggcaaaagtt aaaacaatct tgactgaaaa ggcaggtaag tgaaatgtct
     4981 actaatagtg aatcaaatgc cagaacaatg attggaaaag tagttagcga caaaatggat
     5041 aaaactattg tagttatgat tgaacgtact gtgaaacatc caaagtatgg gaaaatcatg
     5101 aagcgaagaa ctaaactaca cgcacatgat gaaaatcaag tttgccgagt tggtaatacc
     5161 gtaaaaatcc gtgagtcacg accactctct aaaacgaaaa gctgggtatt agtcgaagta
     5221 atttcataag taacttaatt gttttacttt taaaacccta aaaggcctga tataatcagc
     5281 agcctttttc tggtcgaaat tagatctgga gagaaaaatg atccaaatgc agacagtgct
     5341 tgaagtagct gataacagcg gggcacgtaa agtaatgtgt attaaagtac ttggtggatc
     5401 acacagacgg tatgcccgtg taggtgatgt aattaaagta agcgtaaaag atgctatacc
     5461 aagaagtaag gtaaaaaagg gtgctgtaat gagagcggta gtcgttagaa ctgctcaagg
     5521 tgttcgaaga gatgatggtt cattgattcg atttgatgat aatgcagcag ttcttttaaa
     5581 caaccaaaac gagccaatag gaacacgtat atttggcccg gttacacgcg agttaagaga
     5641 aagatttatg aagataattt ctcttgccgc agaggttttg taagaaggat tagatatgaa
     5701 acgtattaaa tcaggcgatg aagtaattgt aattgccggt aaaagtaaag gtcatatagg
     5761 taaggtttta agggtaattg atgatgccgt tgtagtagaa ggcggaaatc taattaaaaa
     5821 gcatatcaag cctaatccac aaaagcctga aaataagggt ggtattataa cgcgtgaagc
     5881 tccattgcac gtttcaaatg ttgctcacta taatcctgtt acaaaaaagg ctgataaggt
     5941 cggctttaaa tatcttgaga gtaatggtgt tagcaaaaag gttagatatt ataagtctaa
     6001 caatgaaata attgaccgta tatagagtta ggtgattgaa atggcgagac ttaaagaatt
     6061 ttataagaaa gacgtcgtca ctatgatgat gaaacggttc aactattcga gtgtgatgga
     6121 agtacccagg attctcaaaa taactcttaa tatgggagtt ggagaagctg ttggtgacaa
     6181 aaaagtcatg aatcatgcga ttgaagacat gacgctgatt tctggtcaga aaccagtcgt
     6241 tacaaaagcc agaaaatcaa tagctggttt taaaataaga gagggatggc caataggttg
     6301 taaggtaacg ctcagacgtg aacgtatgta tgagtttcta gatagattaa tatctattac
     6361 tttacctcgt gtaagagatt ttcgtggttt gaatccaaaa tcttttgatg gaacaggaaa
     6421 ctacagcatg ggaatacatg agcaaatcgt gtttccagag attgattatg ataaaacaga
     6481 cggaatacgc ggtttagata tttgtattac aacaagtgct aaaacaaatg aagaagctaa
     6541 agctttgtta gaagcattta atcttccact gaaagacaaa gatagaaaat aaaagggtga
     6601 aattgtggct aaaaaatcaa tgctgatgcg agagtcaaag cgtgcgaagc tggtagaaaa
     6661 gtacagacaa cgccgaaacg agttaaaaca attgattaaa tcatctgatg atttccaggt
     6721 aataatggaa agtcaggcaa aactagcaaa attaccagtt aattcaaatc ctgttcgcta
     6781 tgttactcga tgcaagcaat gtggaagacc acacgcagtt tatcgcaagt ttaatctttg
     6841 cagaatttgt ttaagacaac agcttatggt gggcaatatc cctggcggta gaaaatcaag
     6901 ctggtaaact attttattgg agaataattg tgagtatgca tgatccaatt gctgatatgc
     6961 tgactagaat tagaaatggt caacaagcta aacatcagca agtaacattg gtttcttcta
     7021 aattaaagga agaaatagct cgagttttaa aggaagaagg atatatacaa gactttttta
     7081 ctgaaacatt accaaatggt ctgaagtcga ttactttaaa actaaagtac tatcatggaa
     7141 gaccggtaat tgaatttatt aagcgcatta gtcgtccagg attgagagta tataaatcat
     7201 ataaggattt gcattcaatc cctggatttg gtgtggctat tctttctacg tctaaaggta
     7261 taatgaccca tgtatcagca aaagtaaaag gtgttggtgg cgaagtcatt tgtgaagtgg
     7321 cttaatactt gcgaggaatg atatgtctag agtagcaaag gccccagtaa ttcattcagc
     7381 aaatgtcgaa gtaacatttg ttgatggagt aataactgta aaaggcccca aaggaatatt
     7441 aactcaaaaa attaataaat tggttaatat tcaacatagt aaggaatcaa acaaattaga
     7501 attttcacct gcttctaatg acccaatggg atgggcacaa gctggtacgg ctagagcctt
     7561 agttagaaac atggttcaag gagttaccga gggatataca gttacactag aattggttgg
     7621 tgtaggatat agagcgcaat ctaaagacaa gtcaattagt ttgtcattag gatactcaca
     7681 ttctatagag tatgatttac caaaaggtgt aacagtagaa actcctaata acacaaccat
     7741 tttgctgaag ggcgttgata agcaagttct tggacaaata gcttctgaaa ttagagcatt
     7801 tcgaccacca gagccttata aagggaaagg tgtgaaatat gctggagagc agattgttcg
     7861 taaagaagct aagaagaaat aaggtgttaa ctatgaataa gcaaaacgca cgacatagac
     7921 gtggattaaa agccaaagcc ttaatacgca aaacaggccg ctcaagatta gttgtgtatc
     7981 gaagtggtgt gcatatttat tctcaaattg tcaagtcaga ccagcttggt gataaggtgt
     8041 tggtagcatc atctactata gataaagaat tgagatccag tttgactgga aaaagtaaag
     8101 ttgaacaagc cagtttagtt ggtcaactgt tgggtaagcg tgctaaggct gcaggcatta
     8161 cacaggttgc atttgacaga gcaggttata aatatcatgg cagagttaaa gcacttgctg
     8221 aaggtgcacg cgaagccgga ttagattttt aaggaacgat tatgtcattc gatgaattac
     8281 ccaagtcaga tggataccaa gaaaagttgg tatcggtaac tcgtacggct aaagttgtaa
     8341 agggtgggcg agtttttggt tttgctgttt tagtagttgt aggtgacgga aagggtaaag
     8401 taggttttgg acgaggtaag gcaagagagg ttccaatagc aattcaaaaa gctatggacc
     8461 aagccaagaa aaatatggtt tacattcctc tttcaggcac tacaatattc cacgaaatca
     8521 catggaacta cggagcttcc aaggtgttta tgaagccagc aagtgaaggt acaggaatta
     8581 ttgctggggg cgctatgcgt gccgttttgg aagtgttggg tgtgcaaaat attcttgcaa
     8641 aaagcattgg ttctaccaat ccaagtaaca ttgttcgtgc cactattggt gcattgacac
     8701 acattggtac gccggactat gtagcagcca aacgtggaaa aacagttgaa gaagtaatgg
     8761 caggctaatt atgggaaaaa aaattaaaat aaccttggtt aaaagcacga taggtagaaa
     8821 accaaaacat gttgctatag ccaaacagct gggtttaggt aaaacaaatt caagtgtggt
     8881 tcatagtgat actcctgcaa ttcgtggact tgtaaatgaa attaattacc tgttgttggt
     8941 tgaggagagt gcataatgaa tttaaataca ttatcgccag atccaggttc cagaccctca
     9001 cgaagacgag tggggagagg tattggttct ggattaggca aaacttgtgg caaaggtcat
     9061 aaaggtcaaa aatcaagagc gggtggttac cacaaaatta attttgaagg tggtcaaatg
     9121 cctatacaaa gacgcttacc caagatgggt ttcaagtcta gagtaggtcg aaccattgat
     9181 gaagtgagtt tgggtgagct tgcaaaattg aatgatgaag taattgattt agttgctttg
     9241 cgtaaagcag gtttaattaa taactcaatc aaagatgtta aagtaatttt atctggcgag
     9301 ctgactgcag caattaagct taagggttta agagtcacta aaggagctcg ttctgctatt
     9361 gagagtttag gcggtagtat agaagagtga ctatgaaaaa ccaaaagcat aacggtagta
     9421 agcctcgtgg tggattagcc gaactaaagt caaggctgtt gtttgttgtt ctcggcatat
     9481 tagtttaccg attaggtgct catatacctg ttccaggatt agatcctgca aaattagcta
     9541 atttttttag tgaacagcaa aacacaatat ttggcttgtt taatatgttt tctggtggag
     9601 ccctgtctcg tgtaacagtg tttgcgattg gtataatgcc ttatatttct gcatcaatta
     9661 ttattcaact gttttctgta gtttctccca agctggagca actaaagaaa gagggagaat
     9721 cagggcgtag aaaaataaat caatatacca gatatttaac attgctcttg tcaatatttc
     9781 agtccatggg tatggcgaga tggttggcta gtcagcaaat tgctatgcaa ccagatttct
     9841 cattctattt tactgctgtt gtaactttag ttacaggtac tatgttttta atgtggctgg
     9901 gtgagcaaat taccgaaaaa ggtgttggta atggaatttc cttaattatc ttttctggaa
     9961 ttgtgtctag catgccaaat gctataggat ctgtcctcca gcaagttaag gaaggtcaga
    10021 tgcaagccct tacactagtt attattgcag cagtagtaat tgtagtaact ggttttgttg
    10081 tatttatgga gcgtgctcaa cgacgaattc gtgttaatta tgcacagagg acgcaaggta
    10141 gaaaggtata tgcagcccaa acaagtcatt taccattaaa aataaatatg tcaggtgtga
    10201 ttccaccaat atttgcatct agcattattt tattgccagc aactttagct caattttttt
    10261 cacaaactaa agggctggga tggttatctg atgttggaat ggcactatca ccaggtcaac
    10321 ccttatattt gatagtgtat gcagcagcta ttttgttctt tgcattcttt tatgctgcct
    10381 tagtgtttaa ccctaaggat acagcagata acctgaaaaa atcaggagca tatattcctg
    10441 gaattcgtcc aggagagcaa acaaccaagt atatcgattc agtgatgact cgtttgactt
    10501 tagttggagc catttacttg gttctagtat gtttgttacc acaaattcta atgtatacct
    10561 ggcatgttcc attttatttt ggtggtactt cgttgctgat tatagttgtt gttattatgg
    10621 attttgtagc acaggtacaa gctcatttaa tgactcagca atatgattct ttattaaaaa
    10681 aggcgaattt tagaggtact aagttacctg gtctgctatg atttatcgga gttaggaatg
    10741 aaagtaagag catcagtaaa gcgaatatgt cgcaattgta aaataataaa aagaagtggc
    10801 actatacgag taatttgtaa ggatgcccgt cataaacaaa agcaagggta atatctgctt
    10861 gattttattt tattaaaata gtatccttct gtccctttcg acagaacaaa taacgttcgg
    10921 agaaactaat ggctcgtatt gcaggagtta atatacctga tcataaacat gtggtaattg
    10981 ctttaacagc aatttatggt attggtaaga caacatcatt aaaattatgt aaaacagttg
    11041 atatagatcc ttcagttaaa gtttctcaat tatcagatgc tcagttggaa tctttgagaa
    11101 ctgaaattgc gaaaattact gtagagggtg atcttcgacg tgttgtaaca atgaacataa
    11161 aaagactaat ggatcttggt tgttatagag gattaagaca cagaagagga ttgccgttgc
    11221 gcggacaacg tacgaaaact aatgctcgta ccagaaaagg ccgtagaaaa ggcactagta
    11281 gttgattttt catgtaggaa aataaaagat ggcaataaca aagtcaaagc agcaaaaaac
    11341 acgaaaaaag gtaaagcgcg tagtgtctga tggcatagta cacattcatg catctttcaa
    11401 taatactata gttaccttta cagatagaca aggtaatgca ttatgctggg caacttctgg
    11461 tggatctggc ttcagaggtt ctagaaaaag tactccttat gctgcgcaag ttgcaactga
    11521 gcgagctgct gctgttgcta aagaatatgg tatgaaatct gttgcagttt ttgtgcacgg
    11581 tcctggtcct ggtcgtgaat ctactattcg tgaattaata acccaggatt ttaaaattgt
    11641 tgaaattacc gatgtaactg gcataccaca taatggttgt aagccaccta aaaaacgtcg
    11701 tgtataagac tcaggagtaa ttaatggcta gatatcttgg tcctaaatgt aaattatcac
    11761 gacgtgaagg ttgtgattta ttacttaaaa gtggggttcg tgatcataaa tccaagtgta
    11821 aatctgaaaa gttacctgga cagcatggtg ataaaaaacc tcgtttaaat agttatggaa
    11881 ttcagcttag agagaaacaa aagattagaa ggctttatgg gattcttgaa aagcaattcc
    11941 gtaattatta caaaaaggcg gctagacaaa agggctctac aggtgaaaat ttaatggccc
    12001 tattggaaag acgtttagat aatgttgttt atcgtatggg ttttgccagt acacgtgctg
    12061 aagctagaca acttgttgca cataaggcaa tacttgtaaa tgataaagta gttaacgtac
    12121 cttcttttct ggttaatcct ggggatactg tatcagttcg acaaaaggct aaaaaccaag
    12181 gacgtatcca agctgcttta gctttatctg aacaaagagc accctgtgat tggattactg
    12241 tagatactgg ttcttttaaa ggtacatttt ctacagcacc aacgttaatg gatttatctt
    12301 cagactacaa cgtaaacctt gttgtagaac tttactctaa gtaagctcgg agagatagct
    12361 gaatgtatac tgaaatcaat gaaatgttga cgcctaaggt tctcaaagta caggccgagt
    12421 cgccctataa agctagaata gttttagaac ctttggagcg aggttttggt cacaccctcg
    12481 gcaatgcttt gagaagaatc ttattgtcat ccatgccagg cagtgccatt actgaagctt
    12541 ctattgatgg tgttttgcat gaatatagta ctattgaagg tgttcaggaa gatgtggttg
    12601 atttgttgct taacctaaaa tcagttgcta tcaaattgac tgtaggaaac gaagcgcagg
    12661 ttaccctaaa taaagaagga ccatgtcaag ttacagctgg tgatattcag ttgactcatg
    12721 gtcaagaaat tattaatcca gaacttgtca tagccaatct caatgaaaag ggtaaattaa
    12781 atatgactct taaagttgaa agaggtattg gctttcataa tactgatgca tttgttcgct
    12841 atcatgaaga tgaaattgag aaaaagacgg ttggaaagtt aaaaattgac aacagttttt
    12901 ctccagtgaa gaaagtagct tatttcgtcg acagtgcacg tgtagaaaac agaacagatt
    12961 tagataagct tactattgaa ttagaaacta acggtactat cgatgctgaa gaggcaataa
    13021 gaatatctgc tagtatactg caaagacagc ttcatgcgtt tgttgacatg aaatttgaag
    13081 aatcacgtgc tgacaataaa gagcgtaatg attttgatcc tgtattatta cgttcagtgg
    13141 atgatttgga gttgactgtt cgatcagcta attgtttaaa agctgaaaac attcattata
    13201 taggcgattt ggttcaaaga acagaaagtg aattattgaa gacacctaat cttggtaaga
    13261 aatcactaac tgaaattaaa gacgttttag cgtcacgatc attatcgctg ggtatgaaac
    13321 ttgagaattg gccgccagca agccttggcg agtaatattt aggagtcttt gttatgcgtc
    13381 accgtaattc cggacgtagt tttagccgta ccagtagcca ccgtaaggct atgttttcta
    13441 atatgtgttg ttctttgatt gagcacgaat taattagaac tactttgcca aaggcaaaag
    13501 atttacgtcg atatatcgaa cctttgatta ctgttagtaa atctgattca gtagcatcac
    13561 gtagacgagc tttcgatatt ttgcgatcta aatcagctgt aggtaaatta tttactgatt
    13621 taggaccacg tttcgcaaaa cgaccaggtg ggtacatacg tattattaaa tgcgggtata
    13681 gggatggtga taatgcaccc atggctatag ttgaattaat ggataggcct gtaagcagtg
    13741 atgatacaga agagtaaatt ttagattaat aactataatt tatattttga gcccgcatta
    13801 acgcgggctt ttatttgaat catttaaaac ggtatgtcat catctaattc atcaaaagca
    13861 tcttgtgcag tagatgttgg tgttgcttga ttttttgttg aagcttgttg aggggtagag
    13921 gttccctgaa atgaaggcat gtcatcgtaa ttagtggcac catttccttt gctatcgagc
    13981 atttgaatgt cagacgcaac aatttcagtt gtatatctat cttggccctg ttgatcttgc
    14041 cattttcttg ttcttaagct cccttcgaca tatagcttgg aacctttgcg aatgtattct
    14101 ccagctattt cacccagacg attaaaacag acaactctgt gccattcagt tctatcttgt
    14161 ttttcacctg tcgttttgtc tttccatgtt tcactggtag caactgaaag tgtagtaaca
    14221 gcatttccgt taggcaaata ccttacatca ggatctgccc ctacattacc aacaagtatt
    14281 actttattta ttcctcttgc cattgaaatc atctcctgta tgcgaaagtt agttaggtat
    14341 tatacataat ttggcagtta tttcgtagca gcccaaagag aaggtgttat tggtaaatag
    14401 tagactgatg aagaattttt tcagcgcttc ctgtggagta ttcctcatta tctactcgaa
    14461 catacaaggt attttcatcc ttggacaaaa taacttcttt aatgccggtt acctttaaaa
    14521 gctgattgac tactaatttt gattcttcat tatttgctgt aaaaggcaaa ataagagtag
    14581 aaaaataaac attagatttc atgctcagcg aaatgatgag ccatagacag ctcagaatca
    14641 tgttaattag aaaaatagct tggttatcgt accactgata aagaatacca gctaaagacc
    14701 caccaaaaaa aattcctaaa aattgactcg ttgaataaat acccattgct gtgcctttgc
    14761 tccctggact tgccagcttt gacactaatg aaggtaatgt tgcttcaaga acattgaagg
    14821 aaacgaaata cacaaacatt aaaatacaaa accaatacca attttgtgat gtgaacacca
    14881 atagaccttg agtaaaggta gtcagcagca cagatataac aaatactgtt tttaatcgtt
    14941 tttttctttc tgaaataatg ataaagggta ccattaaaat aaatgagaat aacatgagtg
    15001 gcaaatagaa gtgccattgc tgatgcaaat gaccagtctc aatttgatat ttcaacagca
    15061 taggtatagc aaaaaaagtg gctgtaagaa taaaatgctg acaaaaaatt ccaaaattga
    15121 ggcgttgtaa atgttgatct gttaccacag gttttatgag agacaagctg ggttcactat
    15181 ctgcatgaaa ctgctctttt gagggattgg gaatgatgag ataaagtatc ataatcccta
    15241 gaacagctaa agctgcagtg agatagaaca ctcctgataa tccatagtag tgggtaagag
    15301 cagggctaat tatcattgcg agactaaatg atgttccgat agttatccct actatggcca
    15361 ttgcctttgt acgttgttca tccagggtta aatcagcaag caaagcaatt aacacgctac
    15421 ctatagctcc ggcaccctgt agtattctgg cagttatcat tccagcaata gaatgagtta
    15481 ctgctcctaa caaacttcct ccagcgaata gcaatagacc tatggcgatc ataggttttc
    15541 tgcctagttt atctgaaagc ataccaaaag ggatttgtag taatccttgt gccaatccat
    15601 agcttcccaa agccaagcca attaatgttg gagtagcacc tttaagatta gtggcataaa
    15661 gagtgaatac agggataagt aaaaataatc ccaggagacg aaaagagaat attgctgcta
    15721 taggaaaaac agttttgttc caagaatact tcattcatta taggtctaaa ttaaatcatg
    15781 tggagatggt acaaattaag tgctttggaa acaagataaa ttattaaatt ccgcttgatg
    15841 gagccccgat atttccattg acagcacagc agaataacgg tagtttggtg ggattttata
    15901 ctgtagggtt attgtatgag ttcattagtt ttttcaggaa aagtgggatt gataacgggt
    15961 ggtgctcgag gaataggtaa agcaaccgca ttaaagttag cgcattcagg ctgcgatatt
    16021 gctatcgtgt attataatag ctctgatgag gctcaaagct tggtcaatga aataacaagc
    16081 atgggtagaa aagcaattgc tttacaagcc gatgtagcag attgtcaatc tgtcaaggaa
    16141 atgttctctc aatttagaga acactttaag cacttaaatt ttctagtgag caatgcagcg
    16201 agtggagtat taaaacccgc tttgaaaatg tcgaccaagc attggcgctg gtgcttggaa
    16261 acaaatgctt tggctttgaa ccacttggtt acggaaggtc ttgatttaat gcctaaaaat
    16321 agccgagtta ttgcgctatc cagcttaggt gcatcaaggg caattccaaa ttatgctttt
    16381 attggcgcct ctaaagcagc tctggaagct ttggttcgtt cgctaagctt ggagctggct
    16441 agttatggta ttactgtgaa tactgtttct gcaggtgttg tcgatacgga cgctttaaag
    16501 cattttccaa acagagaaca gttactcgat gaatatcaag ctcatgcttt ggcagataga
    16561 gcgttaacac ctgaagatgt ggctaatgct atctatttac tttgtttacc tgaagcggcc
    16621 atgattaatg cccatacatt atatgttgac gcaggctata gtcaggttgg gtagtagatg
    16681 ataaaagtaa attttatgat ataatttcaa gctttgcgag ctttgttggt tcagaggata
    16741 aaatatgaat gtagctgaag tttaccctaa ggttagggaa attattgctg atgtattagt
    16801 aattgatgaa gaagaaattt ctttaaacag tagtttaatt gaagatttgg gtgctgagtc
    16861 catcgatttt cttgatttgg tttttcaatt ggaaaaagaa ttcaagatta aaatcccgag
    16921 aggccagttg gaaaagaatg ctcgtggtga tctggctgag gatgaatttg agaaaggtgg
    16981 tattttaaca acctcaggat tacaggcttt aaagaattat ttgagcgaag ttcctgctga
    17041 tcgatttaaa gagaatatga aagttaacga aattcctgtg ttgtttaccg tagaaacttt
    17101 ttgcaagctg gttatttcag ctatagccca gcagcaagca acggaaactg tagcctgatg
    17161 aggttcttat ttgttgatcg cattgtacaa ttatccccag gagaatggat taaagggata
    17221 aagcatgtca caagagatga tgcttattta accactgatg atcaaggaag gctttgcttt
    17281 atgccttctt tgattggtga aacattgggg caattggcag cgtggaatgt gatgcaacat
    17341 aatcatttta cttcaaggcc agtcgctggc gtagtatcaa gtgcccgatt gtatcgacct
    17401 gcttacgtcg gtgaaacgat attgcttgaa tcaaatattg attcactgga tgaaaaagca
    17461 gttcaatacc atagcattgc caaagtaggt aatgatatta tttttactat agatggagca
    17521 ttaggcccca tgctgcctat gactgaattt attagtacag aggtcattag acagcagttt
    17581 gcagagatat acaggccagg tgagtggtct tctgtgttag ctttgagttc gcctgtttta
    17641 atagaggaaa actgctctga ttgtggaatg attaattctt ccatgcaatt tgacagagtt
    17701 ctcgccagtg aaccaggtat aagcttgatt gctgaaaaaa ggataactcg ttcagcatct
    17761 tatttcccag atcattttcc taacagacct gtactaccga tgacggtact tcttgaatgt
    17821 aaattgaatt tggcgaaaga atttatagct agagcaaaat atgatagagc ttatcgaatc
    17881 agcgaattac gtaaaattaa aatgaatgaa tttgttcatc ccggagatgt cgtagtttgc
    17941 caggtcaaag tgaaacagca aatggatgat gaattaatac tgtcttatag gtgtgaggtt
    18001 gatggcaagc gggtatgtgt tgtcgacata gtgcttatcc caaaaggtaa atgaggttat
    18061 gaaacgacga gttgctatta caggtctagg ggttgtttcg cctctaggaa atgatgtatc
    18121 caccacttgg aacaatttga tcgccggcta ttctggcata gatagtatca agcagtttga
    18181 tgccagtgct tttcctacca ctattgctgc tgaagtaaag aacttttcac ctgatacgtc
    18241 tatttcggga aagcatacac gatttatcat gtcttttact cattttgcat tggaagctgc
    18301 acgccaggct tttgaggatg cttctatctt ccctaccaag caaacatccg atcggtgggg
    18361 aattgtaaca ggcagtggga tgatgacggc cgagtttgag tatttaacgc ggtttcaaca
    18421 aacttgtgct gcagacggtg aaattgattg gggaaagctg caatctaata gcagggattt
    18481 ctataatttg gttgattttg gtaaaaccac ttcaaactct ggtctttcct tattaataca
    18541 acaatatggg attactggtt atgcctcttc tgtccatacg gcctgcgctt caggaggcca
    18601 agccttggga ttggcaatgc aggtgattcg tcgtggcgag gcagatttta tgttggcagg
    18661 tggatttgat tccatgatta atcctttggg tttgtcgagt ttttgtcttt tgggtgcctt
    18721 atcaacttat aatgagactc cgcaaacggc aagtcgcccc tttgatgcga cacgcaatgg
    18781 ttttgtcctg ggtgaaggag ctgccttttt gatccttgaa gaatggagta aagcaaaagc
    18841 tcgtggggca aaaatctatg ctgaactggc tggagagggg aattctttga gctcctaccg
    18901 aataactgat tcacatccca acggcgatgg tgctattcaa gcgattaaac gtgcgttgga
    18961 agatgccggg gttcaaccac gggatgtcga ttatattaac gcgcatggta catccactaa
    19021 aatgaatgat ttaagtgaaa ccaatgcaat caaagttgtc ttcggggata ggatcgccaa
    19081 tttacctgtt agctctacca aaagccaaac ggggcattta atcgcagcgg cgggagcact
    19141 tgaagcggta ttgtcagtta aatccataga acaggcgcaa gtccccaaaa cagcgaattt
    19201 aaaaacacct gatccggagt gtgatttgga ttatgttgtt gatggcccac gtgaaaaatc
    19261 tttaggggta gtattatcta attcgtttgg ttttggaggt tctaatagct gcttgttatt
    19321 taaacatcca gaattcgagg aggcaaaata aatgagtcaa gcgaatagag tatttattac
    19381 tggtcgcagc gctctaactg cctctggtgc aacagctgat gaaacttgga atgcaatcat
    19441 ttctggccag tgtggaatag atgaaattaa tttttgggaa ttatctgaat ggtcgcatcg
    19501 tttgggcggg gaaataaaag atttccaacc ttccaagatg cttcctgata gaaaattaat
    19561 aaaagttatt tctcgtcaag atataatggg aatcaatgcg gcagtgcaag ctgttgagca
    19621 cagtaaaatg attgcttaca gagatagcct ggcagatgct gatttattta atgaacaaac
    19681 cgccatttat gttggttctc cagggaataa gtattttcaa caatatgact ttataccttt
    19741 gttggccaaa acccatggag atatgcgtag ctttgcaaat cagttgtttg atgaagtgca
    19801 tcctatgtgg ttattgagga ttttacctaa taatgtgctt gcctatacag gtattaccta
    19861 tggctttaaa ggtccaaatc acaatgtaac gaatcatgcg gtaggtggta cacaagcgat
    19921 tttggaagcg taccatgcca tacgtagcgg gcaggcagaa cgcgcggttg tggttgccta
    19981 tgatataggc acagaacctc aaggcttatt ttattatgaa aaattgggtg tggtaagtaa
    20041 tcgtcattta aaaccttttg ataaagagca tgacggtacc attcttgctg aaggtgctgc
    20101 ggcccttgta ttggaaagtg aagccagtgt gaagtctagg gctgcgactt gttatggcga
    20161 gatagttggt ggtttatccg cttctgaagc aacaggatta ttttctattg aagcagatgg
    20221 gcaacatttg gcttcattga tgaggcagac attaagcaca tcaggaataa gttctgagga
    20281 aatagggtta atagtaaccc atggtaatgg aaacagtaaa tcagatgaca gtgaagctct
    20341 ggctatcaaa tcagtattca aacaagaaga agttcctgcg accgctttta aatggtccat
    20401 gggacatact ttatgcgcct ctggagtatt ggatgcggta ttaactacat atgcccttga
    20461 aaccaaatgc gtgccgggga tcgctaattt tcaacaaatt gccccgacgt gtgaaggctt
    20521 gtcagtaagt cgtgagcata gagagacaat atctaattcc tatgccatga tgatcaaccg
    20581 agggtttgcc agtatgaatg tatgtttggt gatcaaagct tgtgaataat ttagctcaac
    20641 aaatagaagc attacccatt acactggcgg gacgttttat ttatcggttt ttgccataca
    20701 ggcgccggtt gattttttcc aatattagcc aggtttataa tgatcaactg aatgaatctc
    20761 aaaaaaagcg cttggcaaaa gcgtattatt ctcatcttgc aaaatcttta aaagaagcgc
    20821 tgcaattaag attcatgagt gaaaaaaaat tacgagctca ggttgaagta atcggtcatg
    20881 aaaaaatgtt ggctgttgtg gcacagaaaa aaggagtgtt agtagttacc ggtcactttg
    20941 gaaattggga gtttgcgccc ttgggaggag tattaaactt caaggaattc aaaggccaat
    21001 ttcatttcat caggcgtacc ttgcgattta agttcataga acgcattatg ttcaaaaatt
    21061 actatcaagc tggcctaaat gttattccta aaaaaaattc tctggagcag gtttgtgttg
    21121 cgttagaaca aaatcatgcc gtaatcttcg tattggatca gcatgcatcc ctggtgaaca
    21181 gagatgggat agcggtagag ttttttggaa aaaaagctgg cacctacaga agcctggcga
    21241 cgatatctcg ccatacagga attccagtta tacctgcagc cagctacaga ttacctaacg
    21301 gaaagcatgt tttggagttt catgatccca ttccttggaa agattatgac actacgcaag
    21361 aatctttata ccgaaatact ttggcctata atcaggcttt ggaaaagatt attctggcac
    21421 atccggagca gtggaattgg atgcacaaac gctggaaact ttcttaaatg aatggattgt
    21481 tttcggtttc ttatttattc tttgtcacag aatgttttct cccggctcct acttaatcac
    21541 gaatgacttt ttatggggca agttatgtca aatttttaag ttttattttt tcgttcgtct
    21601 cttaatcgtt tgagtaccag aaataactta ttcgtttcat cttcttttgt gaaatgaagg
    21661 acaatgttgt tgtcttcagc tcgaatcact tcatagcaaa gttccataat aaagttatgg
    21721 atttgtagtt taagcactcc ctctttagga atatcagttt tctgcttaac tactacagca
    21781 ccattggaag agatttcaac aatctcaatt ggattattgt ctaagctggc atttatttcg
    21841 ccatggtttt ttagccgaaa atattttcgc ctttcgtcca ttgtttaacc ataaattaag
    21901 tgtgcttttt aattttaggt ctattattcc tgataggtca accggatgag cgtgatatct
    21961 gctgattaaa taggaagggt ccaaatttta gaacggttgt ttgtttcagg ttattataga
    22021 atcaatccgg ctgtagatga gcagccggaa gattaatcaa ggtgaaatca ttgttgatgt
    22081 cgattgggga gtggatttat tatttggata aatagctgac atgatgttgt tgctaaaaaa
    22141 tactcccaag ttgcctaatg tgctttttag gctagtagct aactcagttg ctgaatccag
    22201 gatagttgcg cctggagttt gatttgaaga aacagattca cacttatacg gattttgttc
    22261 gggtaaagaa tgagtaaact ctttagtgat agggctgaaa tgagtagagt cttcatcgaa
    22321 ttccttaagc aaagactcaa cttcattacg agcccctttc attagttgca tttctagcaa
    22381 acgaaaacga ttcgcgatta aaagttgctg attttttata agccaggcat ccaatgttaa
    22441 gtcatttttg ctttctagta acagaaagtg aaataggcga tggagttttt gacgaatttt
    22501 aagctcgttt tttggattgg attgcaatga aaacatactc aatgcttcga ttaattcatt
    22561 aagtttttgt tgggtgtaac ctgattttat gtcatcgagg ataatcgaca gatttgtttc
    22621 aagtttggta gtgaaatatt ttctttgttg aagaagaggg gaggctttta tttcgctttg
    22681 ctttattggc gagtcaattg ctgcaacaaa agcaggtaga tttggggaaa aaaaaccagt
    22741 ccagccataa taccaacatc gtttcaatcg tgaaatcagt tgagcgataa atccggtatt
    22801 ttcaccaaaa cttaactcga tttttgcttc agttaaagct tgagtcgcta tactgataat
    22861 ttccttgtgt ttatttggat ttaatttttt tagagctccc tcgcataaat caataactct
    22921 ggcatagctg tcttcgctag cttgtgcccc aaaatgccgg atagttcttt tagcatcata
    22981 gataaataaa gaaaaattaa tttgctcatc atgtaattca ggatagttta gaaacgctgt
    23041 gtatatagct tctctaacag aagctgatat atcgcttaaa gacatgagaa caattaaatg
    23101 atatatcgaa cgccgatgta tttcagtgca cccttcttga acatagtaac ttaaataatc
    23161 attgatgatt ttggaaagta cttgcttgtc gcctttataa tgcattaaaa aataatctat
    23221 cgatttcacc gttcttttgc tttttattaa tttattaatt aaagggtgtt caggtatatc
    23281 attggtcggt gtcattacag agctttcgct aattttgatg ccgcagaaga ggctgcttaa
    23341 aagacttttt tctttcgatt tagttggaat ttttggggct agctggacca gttgtgtcat
    23401 gctcgtgatg tttatgcgtg aatcattata aaataagctc gaatcaccgg attgtgctga
    23461 tgctcgcaag tagtaattgg tcagataggc gattttatta tttaactctt taaactcttc
    23521 cagattattt aaacaaatca ggagttgcat cgcttgaggt gagaattggt aattacggtt
    23581 taataattta taaaccaata acttgatgta agttacatac gcgtcatgat gttggccata
    23641 gagatagcgt tgaatagcgg taattaatcg tttttcttca ttttggttga tcaaaagagg
    23701 atgagtcgga tcaaataatt cgagatgttc caccatattc ataataatgg tttccttttt
    23761 tgtggaatcc attttattaa gttcgtcaac aacatgaaca tctaccaggt tcatcaaatg
    23821 ggctaaaata aaatgcgcat ttggcctgga tgagtaatga tttagccaat aggcaatcat
    23881 atcaggcttt ggatacttaa gtagtatttt ttcaacctgt ttttctgaca attgatgaaa
    23941 atgagggatc gaacagagta aagtaacaca tgcttcaggt tgtagaagtt ctatttgtgc
    24001 cacagtcaaa caactcaggt ttaattggtg aactagtgca ttaagccgag gtggcgagtg
    24061 aaattcactt tgatttaaat gggctagaaa tgatttttct tttaaattgt ccaggtactc
    24121 ctttttgctt aatagacagg taattaataa tgatttaatc tggctatcct gcgctaaagc
    24181 aattaattca agtatttgct ggtagttaaa tttattagcg tttaacgcat agataaaatc
    24241 gagctgactt tctttactat tgttgatgct ggctaaataa tccttattta agatggctat
    24301 ttttaacaaa tcaaaattta atagttcatt tcttttcata tgattctcca gccagctcca
    24361 tgagccgagt ttaataatat caggtaaagt cgtttgatca ggtgcaacta taagacaatg
    24421 attgcaaagc caattgattt gtgaggtggt gaagttttca cgctgaatca atattttttt
    24481 gagggtatag gggagcagct tttgctcgac aatgtgtttc aaaatgagga ctatctctgg
    24541 ggacgattga agttgatcag gatttaattg actgagccat tttataaata tttccgtttt
    24601 aatcagtgga tcattaagcg ataaaaatga ttttagagct tgctcccagg aaagagtttg
    24661 atttagagat ggtctattta aatctgcgat aagtaataag ctggcaagaa tatcattttt
    24721 tgagacaggt ttatcaaaat caaccacttg atcctccttg accgaaatgt caaaactact
    24781 caatcaactt taatacccat ttcgttcatg ttaagccacg gaatataata ttcacaaatt
    24841 ctcccgatgg aatctttaca ggacttaagc aaccccccga atcttttcgt aaaaaccagt
    24901 ttttaattcg gtcatttagt ttatctaaac tccctcatta aaaatatttt ttgccttgac
    24961 cttagcgttt ttcctaagtc ctgttttata tttgtggatt aattcagaat aactgtttga
    25021 aaaaaaaatt caatacttgt tttaattttg tgaattttgt tatgaaagta atgtgccctc
    25081 atacacaaaa gcagcaggtc ctgttaatat aatgggagca gtgggacaag gccattggat
    25141 acacaaatct cctcctggca aatgaacggt gattttgtct gacaggttat aaaataaacg
    25201 tgctattgct gccgcagcaa ccgcaccact accacaagca atcgtttcac cacaacccct
    25261 ttcgtagaca cgtaaattga ttttttcatg attaacaatt tgcataaatc caacgttgac
    25321 ttgttctggg aattgcgggt ggaaactaat ctgttgtccc aaactgttta caggggctgt
    25381 atctatattt tcaaccaaca atacagcatg aggattgcct acacttatcg catgaagatt
    25441 cactgtattg ccgttattta attcaagtga atattctggg gattggcgat cagcaagtaa
    25501 aggaatttca cctggggcta gtctgggaac gcccatattg acactcacgc tgttgtcctc
    25561 attaatgatt aaatccatga gcgtagtttt agttgctact gtcaatttat tttttgctgt
    25621 taaaccataa tatctggcaa aaagagctat gcatcgcgcc ccattaccac attgacctac
    25681 ttcttgccca tcggcgttga agattcgata attaaaatca attccttcag tttgactgga
    25741 ttctattagt aaacattggt caaatcctat accggtatgg cgattggcta gtttttgtat
    25801 ttgcttaaca gtgagttgaa tagattggtt gactccatcc aatacaatga agtcattgcc
    25861 taaaccatgc attttggtaa attttattcc cataagatgt ctctattgat tggttgttgc
    25921 tttcttttct tgttcaggca aatacaaagg tcctttttgc ccacaagctg acaaacacaa
    25981 agaagtcagg gtgacaacga tgagtaataa tgcgaaataa atgtgtctca tgaggtatta
    26041 ttatagagtt aaattttaat tatatgagat ttgctttgaa actcaatgat tcattcttct
    26101 tgttatttta tatgggaata ttttaagctt gagtaagaca gacagatagc ttattccgt
//
LOCUS       DATNSN010000027        25740 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.027, whole genome shotgun sequence.
ACCESSION   DATNSN010000027 DATNSN010000000
VERSION     DATNSN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25740
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.027"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..360
                     /gene="mreD"
                     /locus_tag="VAH76_000969"
     CDS             <1..360
                     /gene="mreD"
                     /locus_tag="VAH76_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEO1385616.1"
                     /translation="YIEYYLPGKFKLTALLIAGLLLDVLLSTVIGEHSFALLLVTWIA
                     STKSRRVQFFSLIQQVCLIGFFCFLYQSIIIFIDALLGFNYNLIMPVASAILGMIIWP
                     WIRVLGYSSLLTRSVYR"
     gene            complement(296..898)
                     /locus_tag="VAH76_000970"
     CDS             complement(296..898)
                     /locus_tag="VAH76_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEO1385617.1"
                     /translation="MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQ
                     SEGLLVLTDDGKLQHRLTHPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPA
                     EAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRHQ
                     IGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK"
     gene            1159..1857
                     /locus_tag="VAH76_000971"
     CDS             1159..1857
                     /locus_tag="VAH76_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEO1385618.1"
                     /translation="MKWVLVVMAIIAQTSCAVNHRVMVGDEPVIIQQTKGQGKTFVHV
                     HHNEQTALKAAKAVIRKEGGSLITLIHSGGRNIVFHLHNQRYEFDPNRIFTDKGIKKT
                     LTQYSYYTPEAHQEVKKLADKIKALLPEGKVIAVHNNSSYSLKDYLPGHELAKDAKAL
                     YMNPDNYFRNFYLVTKLSDYLRLKMQGFNGVLQKPSATDDGSLSVYLAKRDYINVEAG
                     YDQLAEQIKMLLHA"
     gene            1959..3227
                     /gene="icd"
                     /locus_tag="VAH76_000972"
     CDS             1959..3227
                     /gene="icd"
                     /locus_tag="VAH76_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEO1385619.1"
                     /translation="MESSMTYDKIKVPAQGEAITVAADHSLHVPDNPIIPFIEGDGIG
                     VDVTPPMIRVVDAAVQKAYGNKRKISWMEVYAGEKATKVYGSDQWLPKETLDAMKKYV
                     VSIKGPLTTPVGGGIRSLNVAIRQDMDLYVCLRPIRYFNGVPSPVREPWKTDMVIFRE
                     NSEDIYAGIEWQADTPEAKKVIQFLTKEMGVKKIRFPEHCGIGVKPVSREGTTRLVKA
                     AIQYAIDNDRSTVTLVHKGNIMKFTEGAFKDWGYQVARDSFGAKEYQGGPWMEFKNPK
                     TGKQIIINDVIADAFLQQILLRPEDYSVIATLNLNGDYISDALAAQVGGIGIAPGANI
                     SDQMAVFEATHGTAPKYAGQNKVNPGSIILSAEMMLRHMGWYEAADLIIRGMEGAIEA
                     KTVTYDFERGMQGATLVSSSGFADAMIKHM"
     gene            3629..3964
                     /gene="clpS"
                     /locus_tag="VAH76_000973"
     CDS             3629..3964
                     /gene="clpS"
                     /locus_tag="VAH76_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEO1385620.1"
                     /translation="MSRQNLEEIIQTGIADTELSTEISTAIKRPRKYKVLLLNDDYTP
                     MDFVVEVLKHFFHLNEEVAIQVMLQVHFQGKGVCGVFTRDIAETKVALVNEYARMNQH
                     PLLSSMEPE"
     gene            3996..6263
                     /gene="clpA"
                     /locus_tag="VAH76_000974"
     CDS             3996..6263
                     /gene="clpA"
                     /locus_tag="VAH76_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEO1385621.1"
                     /translation="MLNKELEFTLNLAFKEAKEKRHEFMTVEHLLLSLLDNPAAGNVL
                     QACDANIEALRRDLIEFIDETTPRIPEDELDRETQPTLGFQRVLQRAVFHVQSAGKTE
                     VTGANVLAAIFSEQESQAVYFLRRENITRLDVINYISHGVSKYQNNDMHENMNSSMEE
                     DMMTPEGNESPLDSYCTNLNRRAKQGKIDPLIGRHEEIQRTIQVLCRRRKNNPLLVGE
                     AGVGKTAIAEGLARRIVDGEIPDSIRGCVVYSLDLGALLAGTKYRGDFEKRLKAVLKQ
                     LGQQEGAILFIDEIHTIIGAGAASGGVMDASNLVKPLLANGELKCIGSTTYQEYRGIF
                     EKDRALARRFQKIDISEPTIDETFEILKGLKGKLEAHHGVKFSIASLKAAAELSAKYI
                     NDRFLPDKAIDVVDEAGAYQNLLTANKRKKIISVTEIESVVAKIARIPIKKVSARDKD
                     TLRNLERDLKLLVYGQDQAITALASAIKLGRSGLREPQKPVGCFLFAGPTGVGKTEVT
                     RQLANVLGIELLRFDMSEYMEKHTVSRLIGAPPGYVGYDQGGLLTEAVTKNPHAVLLL
                     DEIEKAHPDVFNLLLQIMDHGTLTDTNGRQADFRHIILVMTSNAGASEIVRNSIGFST
                     QDNTNDGLEVIKKQFSPEFRNRLDAIINFAPLDTQTIGLVVDKFIMELDEQLSHKGVT
                     FTVDKSAREWLMEHGYDKAMGARPMARLIQENIKKPLADELLFGKLMHGGHVTLRVKD
                     GKLHFDSHDHREGVC"
     gene            6487..7266
                     /locus_tag="VAH76_000975"
     CDS             6487..7266
                     /locus_tag="VAH76_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO1385622.1"
                     /translation="MLSVIIITKNEEANIRRCLESVHFADEIIVLDSGSTDNTLAIAR
                     EYTDKVFSTDWQGYGIQKDRALQKAQGDWVLNLDADESVSSELRQEMLQAISSDTADA
                     FRIPIQMIFYNQVLKYSGSPKRHIRLFKRENACYSKDIVHEKVLIPVNARVGKLKKPI
                     WHHSFSDVHHVLYKLNKYSSYSAKIRIESNEKVGLVKTFFSALWMFVRCLFLQKGFLD
                     GRAGFLFAVFGAQGAFYRGVKQIYKDRDINKLPSVNHIKEE"
     gene            7269..8519
                     /locus_tag="VAH76_000976"
     CDS             7269..8519
                     /locus_tag="VAH76_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEO1385623.1"
                     /translation="MRLTIMDTKGILLAPFFFISFMFFIPISPTIKSICFICSLIAVL
                     ITPQYRKHIVYAYNTLWGRAAFCLFLFVVIASLWSPAPYSMQWMVIGKYCKLIYLPIL
                     ATGFINPKVRNWSINSYLAAIFITCVISILKAKNIIASEDPGEVFYNHIITGFMVALA
                     AYLTGLLAFQNSGWLRLIYIFMMFLTSYQVLFINTGRTGYLVYFILMLLLLIQKLNFK
                     QALAGIVLFSGIIALAYHHSPVMQTRIYDLIQDIKLLKQHNKNTSIGYRMQFHHYAKQ
                     LMLKHPFIGIGTGGFKYSYSQDVPVPSWGKELTDPHSQYWMTLAEQGLIGLVLLVIFL
                     ASLFITSFQLTSSRPILLGVLFSFCIGSISDTILCYSTAGYLLVVMSALCFGELIEKR
                     RVKGVEEDHLVPLSAHAPAAISQT"
     gene            complement(8520..8894)
                     /locus_tag="VAH76_000977"
     CDS             complement(8520..8894)
                     /locus_tag="VAH76_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurogenic locus notch"
                     /protein_id="HEO1385624.1"
                     /translation="MLFLKIKTNQRTTMNILKPKAFLLASVFALSASPAFAADGCCSK
                     MGGINYCDSSAGRLVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVV
                     CNDGYVSEECSLQKPVEQISVY"
     gene            complement(9025..9621)
                     /locus_tag="VAH76_000978"
     CDS             complement(9025..9621)
                     /locus_tag="VAH76_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO1385625.1"
                     /translation="MINELTASLNMIVTQTQNNLKILGVILLIPWSIFFISYFISNKI
                     LLLGIIPRHILGLPGILLAPLLHANFNHLFFNSIPLLVLSNFILINGVNYYLIVTVMI
                     TLFSGTLIWCFAKRGIHIGASGLITGYWGFLVYNIYQTGTLTTIILGLLSLYYFAGIF
                     LGIFPGEKGVSWQGHLFGLLAGFLTSYLLEVYPQILIY"
     gene            complement(9618..10520)
                     /locus_tag="VAH76_000979"
     CDS             complement(9618..10520)
                     /locus_tag="VAH76_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO1385626.1"
                     /translation="MKKLLHSVACLLILISSAKLSYGLALPENHSVNGGLTIIPIDFN
                     QKPEAYFEGKRIPVLPSTQPRQWLLIVAIPLKNTNSVQYINVTKPIKASIPFHVSEKF
                     YSTQFLNIKDISKVDPQPEDLIRIEKETKKLTQIFANYSNTNPFEKQFTAPLRGPITS
                     LFGLKRVYNKQPRDPHSGLDIAANEGKPVYAVNQGVVADTGDYFFTGNTVILDHGMGV
                     FSVYAHLSKILVKTGETVKQGQELGLVGMTGRVTGPHLHWTMVVNQTLVEPLLFVPFR
                     KITVTPVIPDKKSNKSTQDATVKS"
     gene            complement(10529..11860)
                     /locus_tag="VAH76_000980"
     CDS             complement(10529..11860)
                     /locus_tag="VAH76_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO1385627.1"
                     /translation="MSSQLPILTVSQLNRQVKGFLENEIGLVHVEGEISNLSKPSSGH
                     YYFTLKDSTAQIRCAFFKNRHSNSLLRNFNDGQQIVATGKLSLYEARGEYQLIVEEIV
                     EAGMGILYQRFEELKIKLASEGLFNPERKKTLPRIPETIGIITSPTGAAIQDILSTLD
                     RRFPIARIIIYPSEVQGQTAPQQLVNSLKLANAHKRCQVLILARGGGSIEDLWAFNDE
                     YLARQIAISEIPVVSGIGHETDFTIADFVADYRAETPTAAATAVTPNCIELFNILDTA
                     IYRLHDAIIRLIKGLQLKLNHLIDKIASPRQAISTYWQTLDYLERQLISAMTQFINLN
                     INKVNIFSTQLQASNPKIQIERTKIQLQQLIMQLTQEIRIKVNQLKNQLSTNLSTLHA
                     VSPLATLDRGYAIVSKNQRILFAAQQAQIGDTINIRLAKGSLACEVTQIKD"
     gene            12010..13734
                     /locus_tag="VAH76_000981"
     CDS             12010..13734
                     /locus_tag="VAH76_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEO1385628.1"
                     /translation="MRKALFVVAMLTSSYTTADTLYEAVQYGMIANPDILLNTAKGLS
                     AKQAIDKAKGGLYPSIDVTGGFGRQRSVNPTSAAIDDTPSTTLNIVESAVELRQRLFA
                     GGGIINEVKRNQYLTEAQKWKTQGIAEDLALEITKNYFAVLLHERLYAYSIENLKAHK
                     AVFKMIKERADAGITRTAEVDQANARLALAEANKISALADLQEVKINYAKTVGKWPEN
                     LQMPHVPARKSLPNNLARIIEKGLDNHPTVKSSYADIKEAKAQYDVARAAYYPEVNLV
                     LNSSKNKNLGGLIGPNDSDTVAVRMNYNAFRGGADAARIRETAYQVQEAYETKNRTLL
                     ELKETIRLAWNAYVASALRIQPLKQHVTASRKTRTAYQDEFKVGKRTLLDLLDSQNEY
                     YQSQIELARAENDEILSRYRILNGMGCLLKYLNLRLPVNVVNNDVFSSAQTNILLNRK
                     MDEIPYPNNTDNPMVLTHPVKNMEKTRLTKSMVDKNTTYPQQITPKLWYISAGSFRDR
                     NNAIALVKRLNGLGFTAFMSPYKDLLSVFVGPYEYRGHAGNGMERLKELAHVEGVLVT
                     FKEPPKKA"
     gene            13685..14419
                     /locus_tag="VAH76_000982"
     CDS             13685..14419
                     /locus_tag="VAH76_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEO1385629.1"
                     /translation="MLKESWLLSKSRPKRLSVIAKRFQNVLLESLRYRLVLSIWTGLI
                     LCFITSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWANLIESLKKKPVKIQLEKVN
                     SFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRI
                     TYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLA
                     KTRGESSLMGDSKSLGKWDALMKRME"
     gene            14435..16354
                     /locus_tag="VAH76_000983"
     CDS             14435..16354
                     /locus_tag="VAH76_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1385630.1"
                     /translation="MTLTKKMAVGVLIMLLFIFIGTYFITMNNARNFFIQQLESNAQD
                     TATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVKDIKGKVIILKKQLPQESD
                     IPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFA
                     LVALVLSYCFIKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTK
                     VKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVID
                     GLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFE
                     KECQEFEQILSQGINDIKICKIHMGAASYFLHQPVSNLLSTVDQAVKKARETGVFYCQ
                     KEHDTYKYPQLISGDEIRNSLQQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGA
                     GYFIPVAEKLGLAYLIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLE
                     DTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDL
                     NIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHI
                     PWGQGRFLSSVELIK"
     gene            16490..17215
                     /locus_tag="VAH76_000984"
     CDS             16490..17215
                     /locus_tag="VAH76_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385631.1"
                     /translation="MNYFGNLQICFSKSFLLLLIVSSLTACVDLTGARNTYIKKEKQP
                     AISDVPNKKSKAQNMSSKKGEVHTMLGGLGIFSKGMYTLRDKVSEKYHITAHSTMWYN
                     AGAVSRYIIDHYYQEKDHKPIILVGHSLGANEQIKVARNLNAAGVPVALLVTVDAVSQ
                     TIVPPNVKQALNVYKPGFVPMFSGLKLRAVDPQLTKIDNINVDSIKGIKVNHFTIDKD
                     EILQAMIMDKVDKVLVDGNKKGA"
     gene            17193..18503
                     /locus_tag="VAH76_000985"
     CDS             17193..18503
                     /locus_tag="VAH76_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO1385632.1"
                     /translation="MEIKRVLKDRLSARVCVIGAGPSGIAAIKNLQEHGITNVTVFEK
                     NNQIGGNWVYDEQNEHSSVYETTHIISSKRWSEFEDFPMPVDYPDYPSHSQLLKYFQS
                     YVEHFHLDRYIRFNTVVQKVHRLDDNTWHVIYEDAQGIHEACYDYLLVANGHHWDPFM
                     PVYPGVFDGEILHSHQYKKASIFKGKRVLVVGGGNSACDVAVEISRVAPGTCISMRRG
                     YHIFPKFIFGKPTDVAVAKIQWMPSWLRQKFISLVIRGLQGRYAKYKLMKPDCGPLEI
                     HPTINSELLYFIRHGKIHPRPGISRFEGNTVHFTDGTQGEFDTIIFATGYQISFPFFD
                     KSCIDFSNSTTIPLYRKMMHPEFENLYFIGLCQPQGCIWPLADYQSKIVARIIAGSLK
                     RPDQLHRKIAKEINKPHHRFKSHMRHALEVDYHIFRKELLEMLG"
     gene            complement(18626..19474)
                     /locus_tag="VAH76_000986"
     CDS             complement(18626..19474)
                     /locus_tag="VAH76_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1385633.1"
                     /translation="MNVVIITGAASGIGHALSQVCLSNGKTIVMVDKNQDKLHTEAQS
                     FMTKFSPENVICYACDVTKPSEVERLAEYSCKQLGQVDWIFNNAGIIGNLLPAWELQE
                     NDINQVMQVNLHGMLNVIRSFMPYLFKQNFRSHIINMASLYALCTGSQMAAYSMSKHA
                     VLALSESLYFDLNRLKKPVDVSVVFPSFTDTSLLASSGKLNHSPIHNQLNNLLAHSRP
                     AIEVAEHIVREVEQKRFYILPDKEVKGYCEDRTKAILLQENPHRNSVEQLMCSLLKRQ
                     SSEANL"
     gene            19777..19965
                     /locus_tag="VAH76_000987"
     CDS             19777..19965
                     /locus_tag="VAH76_000987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385634.1"
                     /translation="MNTTEIPEQPIEYPEQPIEYPEEPSEYPEQPLEYPDNPEPLEPG
                     YPELPEPEPSQTPEPGFE"
     gene            complement(19971..20747)
                     /gene="trpC"
                     /locus_tag="VAH76_000988"
     CDS             complement(19971..20747)
                     /gene="trpC"
                     /locus_tag="VAH76_000988"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEO1385635.1"
                     /translation="MNSILERIAKHKLEEVAVAKKNKPLHVLSKQQPGEIRDFITALK
                     SNASPAVIAEIKKASPSKGLIRKDFNVAEIAKIYTQNGARCLSVLTDIEFFQGHPDYL
                     ALAKSKTTLPVLRKDFIIDSYQIYESLVLGADCILLIVALLDDVQLMDFCQLAQELKM
                     SVLVESHTQDELERALRLPTPLIGINNRSLHNFKTDIQLSIQLKQFVPKDKIIITESG
                     INTREDIKLMQSHGINAFLIGESLMRADNIGKALQKLMTD"
     gene            complement(20740..21774)
                     /gene="trpD"
                     /locus_tag="VAH76_000989"
     CDS             complement(20740..21774)
                     /gene="trpD"
                     /locus_tag="VAH76_000989"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEO1385636.1"
                     /translation="MKPKLLFEQLLSRQDLSSDQMQEVIHACMTGEFSDVQIATFLAL
                     MRMKGETVNELTAAAKVMRQLAHKIDLGNPLIDIVGTGGDGRNTFNVSTACSFVVAAA
                     GIKVAKHGNRSVSSRSGSADLLEQAGFILNLSDSQVQNCINQCQLAFLFAPHYHPAMQ
                     HARAARQQLGIRTLFNLLGPLINPAQVKRQVVGVFSTNWLKTIATVLANLGSERSLVI
                     SSQDGLDEISIAAKSEVVEYRDGNFKQWFISPEDYGLKHSSLDAIIVDSPEQSLHLIQ
                     SVLSGDSGPARDIVLLNSAAAIYCAKDGISFDAAIEEAKIAIDSGKANHCFNKLRLLT
                     QTLNKESNHE"
     gene            complement(21752..22330)
                     /locus_tag="VAH76_000990"
     CDS             complement(21752..22330)
                     /locus_tag="VAH76_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO1385637.1"
                     /translation="MLLIIDNYDSFTYNLVQYFQSLNQKVAVFKNDEIQIDAIRQSAP
                     QYLVISPGPNSPDESGISIEVIKEFHKHIPILGICLGHQCIAQAFGGKIIPAPEIMHG
                     KTSEITHHYQGLFTGIPNHFQATRYHSLAVDIMSLPNCFSIDAWADDTIMAISHRQFP
                     LFGLQFHPEAILSQFGLQILENFLRYETQTSI"
     gene            complement(22364..23089)
                     /gene="lptB"
                     /locus_tag="VAH76_000991"
     CDS             complement(22364..23089)
                     /gene="lptB"
                     /locus_tag="VAH76_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEO1385638.1"
                     /translation="MSLLEAKQLKKSFKGRTVVNGVSIHIKQGECVGLLGPNGAGKTT
                     CFYMIVGLQSCDEGHIILNGTDITQDAMHLRARHGISYLPQEASVFRKLTVAENIMAI
                     LELRKDLDDQKREEKLDLLLQEFHISHIENSLGMALSGGERRRVEIARALAIEPSFIL
                     LDEPFAGVDPISVIDIKKIILHLCDKGIGVLITDHNVRETLDICERAYIVSQGKILCE
                     GTPKAILANQQVRQVYLGEEFAL"
     gene            complement(23086..23595)
                     /gene="lptA"
                     /locus_tag="VAH76_000992"
     CDS             complement(23086..23595)
                     /gene="lptA"
                     /locus_tag="VAH76_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEO1385639.1"
                     /translation="MTQLKVKYIILLLLMLCSISGNALPFDKEKMVQVVSDSADLNQK
                     NHQGVYIGNVEFVQGSTNLRAEKAITQGDAKNQLTLAIAEGSKDKQAHYWTTTAPDKP
                     PFHAYADTIKYYPLKHLIELIGNARIEQGPNSLSAAKISYDTQEQHVLSHSDEKTRTT
                     IIIYPEKKS"
     gene            complement(23576..24145)
                     /gene="lptC"
                     /locus_tag="VAH76_000993"
     CDS             complement(23576..24145)
                     /gene="lptC"
                     /locus_tag="VAH76_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEO1385640.1"
                     /translation="MNITKQAIWLFLTLLILAGSGWYYAHFNQAIINKKTLSNEVDTT
                     ISQLTVKQFNSEGVLINLLTTPQVEYIPNNNLHLLKNPHIIIKQDNQPAWEINSLRAK
                     SFDGGKQITFIEQVVVHQNPGENTRESTLKTEEITYYPKEKKATTSLFVTFEQPGNII
                     QSTGMNAYLDEKRVELLHKARGSYDPAKG"
     gene            complement(24142..24669)
                     /gene="kdsC"
                     /locus_tag="VAH76_000994"
     CDS             complement(24142..24669)
                     /gene="kdsC"
                     /locus_tag="VAH76_000994"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HEO1385641.1"
                     /translation="MNELLEKAKKIKCLICDVDGVLSDGLLHIDNHGNELKSFHVQDG
                     MGLKLLMAAGIQVAIITTAQNAVVDHRMEQLGITHYYKGQVDKRSAYQHLKKTLGLND
                     EEFAYIGDDLPDLPLIQQVGLGVAVSNAVPQVLEFADWRTERTGGRGAVRELCDLILN
                     AQNKAELAITGYLKQ"
     gene            complement(24683..25645)
                     /locus_tag="VAH76_000995"
     CDS             complement(24683..25645)
                     /locus_tag="VAH76_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEO1385642.1"
                     /translation="MNFCTLGLAVIETEAQAVFELTQRIDEHFEKACELLLSCKGRIV
                     VTGMGKSGHIANKLASTFSSTGSPAFFMHPGEASHGDLGMITRQDIVVAISNSGNTHE
                     IVTLLPLLKRLEVPLITLTGNKQSTLAKSADVNLDVSIKQEACPLGLAPTTSTTVSLV
                     MGDALAISLLQARGFSAEDFALSHPGGALGKKLLLKIDDLCHTGEQLPLANENATVSD
                     ALIEVTNKKLGMTCVVDNHGYLVGVYTDGDIRRTLTRQFNINTTLIKDVMTKNCRTIS
                     KGMLAAEALAIMQKYSITSLVVVENDNRPYAVLHLHDLLKAGVF"
BASE COUNT         7840 a         4561 c         5239 g         8100 t
ORIGIN      
        1 tatattgaat attatttacc aggtaaattt aaactgactg ctctgttgat agctggctta
       61 ttactggatg tactcttatc aaccgtgatc ggtgaacatt cattcgcatt actattggta
      121 acctggatag cgtcaaccaa atcgagacga gttcaatttt tctcactaat tcagcaggtt
      181 tgtttgattg gatttttttg ttttctatat caatcaatta ttatatttat tgatgctctg
      241 ttaggtttta attataacct gataatgcct gtagcaagtg ccattttggg tatgattatt
      301 tggccctgga tccgagtctt gggttactct tccttgttaa ctcgttcagt ttatcgttag
      361 taaggttgat taacttatat tcccctggct gaagttgatc taaccgccaa tcaccaatct
      421 ggtgtctaat taaacgtaaa gtaggaaatc caatagccgc ggtcattttt cttatttgat
      481 gatttttccc ttctcttaaa ataatttcca gccaggtagt tggtatgtgt ttccgttctc
      541 ttataggcgg aattcgtggc catatttttg gctctgaaat caacttggct tcggcgggta
      601 aaaaagtaat gtctttgatt accaggcctt ttcttaaagg ttgtaaatct ttgtctgtag
      661 gggccccctc tacttgaacc cagtaatgtt tctttttttc aaatttagga tgggttagcc
      721 ggtgttgtag ttttccatcg tcagttaaga caagcaagcc ttcgctttgt ttatccagac
      781 gtccagctgc ataaaaaccc ggaaatttaa taaatgccga gagagtttgt tcgggtaatt
      841 cgccagtaaa ttgagtcagg actccataag gtttattaaa aagtattaat tgtggcattt
      901 tatgattgcg ttgattatgg aaattaatga taaatttttt ggttattgac agcaaccact
      961 tcagggctag atcacgcctt taagcacaat aacagcgtaa ataattgttc atattttgtc
     1021 attcactcaa tgagatatga ggtgcagtaa ttaatcaaaa tatgattttg tccctcttgg
     1081 aattaaagcc aagattgatt ttttatggcc aatcactata caattacaca attttattcg
     1141 ttatggtgac tcttattcat gaaatgggta ttggttgtta tggcaattat cgctcaaaca
     1201 agttgtgcag tgaatcacag agttatggtt ggggatgaac ctgtaataat tcaacaaacc
     1261 aaaggtcaag gtaagacttt tgtacatgtt catcacaatg aacaaaccgc actaaaagct
     1321 gctaaagctg tcattagaaa agagggtgga agtctcatta cacttatcca ttccggcggt
     1381 cgaaatatag tattccattt acataaccaa cgctatgaat ttgatcccaa ccgcattttt
     1441 accgacaaag ggataaaaaa aactttgacg caatacagtt attatactcc tgaagcgcat
     1501 caagaagtga agaaacttgc agataaaatt aaggccttac taccagaggg taaagtaatc
     1561 gctgttcata ataactcctc ctattcttta aaagattatt tgcctggaca tgaacttgcc
     1621 aaggatgcaa aagctttgta tatgaatcct gacaattatt ttaggaattt ttatctggtt
     1681 actaaattaa gcgattatct gcgtttaaaa atgcaaggtt ttaatggtgt tttacaaaaa
     1741 ccttcggcta cagacgatgg atctttatca gtctatctcg ctaaacgtga ttatattaat
     1801 gtcgaggccg gttacgatca gttggcagaa caaattaaga tgttgcttca tgcttagttt
     1861 caatctgagc taatttagca aaaaaaaaat aattaatagt cgtaattgat atttgggaaa
     1921 cctgattatc acatgctatc attaatttct ctaaatttat ggagtcgtca atgacatacg
     1981 ataaaatcaa agtgcctgcc caaggcgaag ctattactgt tgctgctgac cattcacttc
     2041 atgttcctga taatccaatt attcctttta ttgagggtga tggtatcggt gttgatgtaa
     2101 cacctccgat gatccgtgtt gttgatgctg cagttcaaaa agcatatggc aacaagagaa
     2161 aaatttcctg gatggaggtt tatgccgggg aaaaggctac taaggtttat ggtagcgatc
     2221 aatggcttcc caaagagact ctggatgcca tgaaaaaata tgtggtttca attaaaggtc
     2281 ctttaactac tcctgttggt ggcggtatac gttcattgaa tgtcgctatt cgccaagaca
     2341 tggatctcta tgtttgttta cgtccaattc gctattttaa tggtgttcca agcccagtgc
     2401 gggagccatg gaaaaccgat atggtcattt tcagggaaaa ttccgaagat atttatgccg
     2461 gtattgaatg gcaggcagat accccggaag ctaaaaaagt gattcaattt ttaactaagg
     2521 aaatgggtgt taagaagatt cgttttcctg agcattgtgg tatcggtgta aagcctgttt
     2581 ccagagaagg tactactcga cttgtcaaag cggccattca gtatgcgata gataatgacc
     2641 gcagtacagt gactctggta cataaaggca atatcatgaa gtttactgaa ggggctttta
     2701 aagattgggg atatcaggtt gctcgcgatt cttttggagc aaaagaatat caaggtgggc
     2761 catggatgga atttaaaaat cctaaaaccg gtaagcagat tatcattaat gatgtgatcg
     2821 ctgatgcctt tttgcaacaa atcctgttaa gacctgaaga ctatagtgtc attgctacgt
     2881 taaatttgaa tggtgattat atttctgatg ctttggctgc tcaggttggg ggaatcggta
     2941 tagctcccgg agcgaacatt agtgatcaaa tggctgtgtt tgaagcaact catggaacag
     3001 cccctaaata cgctgggcag aataaagtga accccggttc tattattctt tctgccgaaa
     3061 tgatgttacg tcatatgggg tggtatgaag cggctgattt gattatcaga ggtatggaag
     3121 gcgcgataga agccaaaacc gtaacttatg attttgaacg tggtatgcaa ggagcaacgt
     3181 tagtcagcag ctctggtttt gcagatgcta tgattaagca tatgtaaacc ggggagttat
     3241 cacatggtct ttctgaacaa cgcgtattga caatatagtg cttacagcgt tactcagtgc
     3301 caatgcgttg ttcatgaaag actgggatta tcctttcaag attattggta gtatctttga
     3361 ctatcaggtt tatacatctt acgagttcat gaaaattgag caggaacttt atccatgcta
     3421 attatgatgc tactctactg attagagacg gctcaatctc ttgagtaaaa cctggtggtc
     3481 aataacaaat agtacaacat ttgagtgaat catatctaat cactatgcta ttgattattt
     3541 caatgactaa atttttcatt aaaattagct aaatttactg gccaaatgcg tgtagggagt
     3601 ctataaaaat agtaagaggt tgttagttat gagcaggcaa aatttagagg aaataataca
     3661 aacagggata gctgatacag agcttagcac agaaattagc acggctatta aacggccaag
     3721 aaagtataag gttttattgc tcaatgatga ctatacgcca atggattttg tggtagaggt
     3781 actgaagcac ttttttcatt taaatgagga ggttgcgatt caggtgatgt tacaggttca
     3841 ttttcaaggg aagggggttt gtggagtgtt tacacgagac atagcagaaa caaaagtcgc
     3901 cctggtaaat gaatacgcca gaatgaatca acatccatta ttatctagta tggaaccgga
     3961 gtaaactggt ggtgggttgg taaggagcaa tgacaatgtt aaataaagaa ctagaattca
     4021 ccttgaatct agctttcaag gaagcaaaag aaaagcgtca tgagttcatg actgttgaac
     4081 atttgctgtt gtctttgctt gataatccag cagcaggaaa tgtattacag gcttgtgatg
     4141 ctaacatcga ggcattacgt cgtgatttaa tcgaatttat tgatgaaaca accccaagaa
     4201 ttcctgagga tgaactggac agggaaactc aaccaacatt aggttttcaa agggttttac
     4261 agcgtgcagt atttcatgtg cagtcagcag gtaaaacaga agtcactgga gctaatgtat
     4321 tagccgcaat ttttagtgag caagaaagtc aggctgtata ttttttgcga cgtgaaaata
     4381 ttactcgcct tgatgtcatt aattatattt ctcatggagt gtcaaaatat caaaacaacg
     4441 atatgcatga aaacatgaat tcgtctatgg aagaagacat gatgacccct gagggtaatg
     4501 aatctccact tgatagttat tgcaccaatc tcaacagacg cgcaaaacag ggcaagatag
     4561 atccgttgat agggcggcat gaagagatac aaagaactat acaagtgctt tgcagacgtc
     4621 gtaaaaataa tcctttatta gttggcgagg caggtgtagg taaaacggct atcgcagaag
     4681 gtttggccag acgcattgtg gatggtgaaa tcccagattc cataagaggc tgcgtagtct
     4741 actctcttga tttgggcgcc ttattggcag gaacgaaata cagaggggat tttgaaaaac
     4801 gattgaaagc tgtgctgaaa cagttggggc agcaagaagg agccatttta ttcatagatg
     4861 aaattcacac cattattggc gcaggcgctg catctggagg agtcatggat gcttccaacc
     4921 tggttaagcc attattggca aatggtgaat tgaaatgtat tggctcaacc acttatcagg
     4981 aatatcgtgg aatatttgaa aaagacaggg ccttggcaag acgattccaa aaaattgata
     5041 tttcagaacc aacaatcgat gaaacttttg aaatattaaa aggattaaaa ggcaaattgg
     5101 aagctcacca tggagtaaaa ttttcaattg cttcattaaa agcagcggca gaattatctg
     5161 caaaatacat caatgatcgg tttttgcctg acaaagcgat tgatgtggtg gatgaagcag
     5221 gcgcttatca gaatttactg acagcaaata aacgtaaaaa aattattagc gtgactgaaa
     5281 tagagagtgt tgtcgcaaaa atcgctagaa tcccaattaa aaaagtatct gcacgcgata
     5341 aagatactct gcgtaacctg gagcgtgatt taaaattatt agtctatggg caagaccagg
     5401 caattactgc cttggcttca gcaatcaaac tgggtagatc cggacttaga gaacctcaga
     5461 agccagtagg ttgtttcttg tttgccggac caactggtgt aggaaagaca gaggtaacaa
     5521 ggcaactggc taatgtgtta ggcatagagt tgctgcgttt tgatatgtca gagtacatgg
     5581 aaaaacacac tgtttcacgt ttgattggag cacctcctgg ctatgtagga tacgatcaag
     5641 gtggtttact gactgaagcc gtgactaaga atccacatgc tgttctgcta cttgatgaaa
     5701 tagaaaaagc acatcctgat gtttttaatt tattgttgca gataatggat catggcactt
     5761 taactgatac taacggtcgt caagctgatt tcagacatat catacttgta atgaccagta
     5821 acgcaggagc cagtgaaatt gttcgtaact ccataggttt ttcaacgcaa gacaatacca
     5881 atgatgggtt ggaagtaatt aaaaagcaat tcagtcctga gtttaggaac cgtttggatg
     5941 ctattatcaa ttttgctccg ttagatacac agactatagg actagtcgtt gataaattca
     6001 ttatggagtt ggatgaacaa ttaagtcata aaggcgttac ttttacagtt gataagtcag
     6061 ccagggaatg gctgatggag catggttatg ataaagccat gggagcaaga cctatggcca
     6121 gattgattca ggaaaacatc aaaaagcctc ttgctgatga actgttgttt ggtaaattaa
     6181 tgcatggcgg tcatgtcact ttgcgagtga aagacggtaa actgcatttt gacagtcatg
     6241 atcatagaga aggtgtttgt taaacgggaa ccatcttcag ttctgtgcat gacaaatttt
     6301 ttgttatgca cagacaaaat cacaataatc ctgagatagt aattgtcatt aacttgttag
     6361 ttgcataact ggtaattgtt atcacgattt ctttttgtga ccgctaaacg aatattattc
     6421 ctttttacct tgttacaatc cggcttaatt atcgatataa tatttatttt gaattgatat
     6481 aaagccatgt tatccgtaat cattatcaca aaaaatgaag aagccaacat aaggcggtgc
     6541 ttggaatccg ttcattttgc agatgaaata attgtattgg actctggcag cacagataat
     6601 acattagcta tagccaggga gtatacggat aaagtatttt caacagattg gcaaggctat
     6661 ggcatccaaa aggatagggc cttacagaag gctcaagggg attgggtttt aaatcttgat
     6721 gctgatgaat cagtaagctc ggaattacga caggaaatgc tacaggccat ttcatcggat
     6781 acagcagatg catttcgtat tcctatacaa atgatttttt ataatcaagt tttaaaatat
     6841 tcaggaagtc ctaaaaggca tatacgatta tttaaaagag agaatgcttg ttatagcaaa
     6901 gatatcgttc atgaaaaagt attgattcct gttaatgcga gagtggggaa actaaaaaaa
     6961 cctatttggc atcattcttt tagcgatgta catcatgtgc tatacaaact taacaaatat
     7021 tcttcttata gtgcgaaaat ccgtatagaa tccaatgaaa aagttggatt agtaaaaact
     7081 ttttttagtg ccctatggat gtttgtcaga tgcctttttt tacagaaggg atttttagac
     7141 ggcagggcag gttttttgtt tgcagtattt ggtgcgcaag gcgcttttta tcgcggggtc
     7201 aaacaaatat ataaagacag ggatatcaat aaattaccca gtgtaaatca tataaaagag
     7261 gaataatcgt gcgattaacc ataatggata ccaagggtat tttattagct ccttttttct
     7321 ttatctcatt tatgtttttt attccgatca gtcctacgat aaagtctatt tgtttcattt
     7381 gcagtttaat tgctgtattg attactccac aatacagaaa acatattgtt tatgcttata
     7441 atactttgtg gggacgcgct gcattttgtt tgtttctctt tgtcgttatt gcaagtttat
     7501 ggagtcccgc tccttattca atgcaatgga tggtcattgg taaatattgt aaattaattt
     7561 atcttcccat tttagctact ggttttatta atcccaaagt aagaaactgg agtatcaata
     7621 gctatttggc tgctattttt attacttgtg ttatttccat cttaaaggct aaaaatatta
     7681 ttgcgtcaga agatccgggt gaggttttct acaatcatat tattactggt tttatggtgg
     7741 cattggcagc ctatttaaca ggattgctgg cttttcaaaa ttcgggctgg ttacgactta
     7801 tttatatctt catgatgttt ctaaccagtt accaagtgct ttttataaat actggaagaa
     7861 cgggttattt ggtctacttt attttgatgt tattgctttt gatacaaaaa ttaaacttca
     7921 aacaggcttt agcaggaatt gttctcttca gtggcatcat tgctttggca tatcatcata
     7981 gccctgtaat gcaaaccaga atttatgatt taattcagga tatcaagtta ttaaaacaac
     8041 ataataaaaa tacatcgata ggctatagaa tgcagtttca tcactatgcc aagcaattga
     8101 tgttgaaaca tccttttatt ggaataggca caggtggatt taaatacagt tattcacaag
     8161 acgtacctgt cccaagttgg ggtaaagagt tgacagatcc acacagtcag tattggatga
     8221 ctttggctga gcaaggttta attggtctgg tacttttggt aatattttta gcgagtttgt
     8281 ttataacatc atttcaattg actagctcca gacctattct tttaggtgtt ttattttctt
     8341 tttgtatagg ttctatttcc gacacgattt tatgctattc gactgcaggt tatttgttgg
     8401 tagtgatgag tgccttatgt tttggtgagt taattgagaa gcgtagagtc aagggcgtag
     8461 aagaagatca tcttgttcct ttatcagccc atgctccagc agcaataagt cagacctaat
     8521 caataaacag aaatttgctc aacaggtttt tgcaatgaac attcttccga aacatacccg
     8581 tcattgcaaa ccacaagacc cgatgaattt aattgtggat agaccccgcc atgccataag
     8641 caacatccca taagaaattg taaatccatt acggcatgac gtgtgcagta gcaagttgaa
     8701 tagaaaccgt tgttacaaac aagacgacca gcagaagaat cgcaataatt tattcccccc
     8761 attttactgc aacaaccatc agcagcgaaa gcaggagatg ctgacaaagc aaacacactg
     8821 gctagtaaaa atgcttttgg tttcaatatg ttcattgtgg ttctctgatt ggtttttatt
     8881 tttaagaata gcatagagtt agatggatta ttagtgaaat atgatgattt aattgcagtt
     8941 taagactgaa tttattatga caaaaaagtt caaagtgaaa tgataaactg gtttcatggg
     9001 ccagctattg tgaagagtta atgattaata aatgagaatc tgaggataga cttctaataa
     9061 atagctggtg agaaaacctg ctaatagccc aaacaagtgg ccttgccagg atacgccttt
     9121 ttcacctgga aaaattccca gaaaaatacc agcaaaataa tataggctaa gcagaccaag
     9181 aataatagtg gtcaaggtac ctgtttgata aatgttatac accaggaatc cccagtagcc
     9241 tgtgattaaa ccactggcgc caatgtgtat acctcgttta gcaaaacacc aaatcagtgt
     9301 tccgctaaaa agagttatca tcactgtgac aatcaaataa tagttaactc cattaatcag
     9361 aataaaatta cttaaaacca gtaaagggat agagttaaaa aacaggtgat taaaattagc
     9421 gtgaagtaaa ggtgctaata gtatccccgg gaggcccaaa atatgccttg ggataatgcc
     9481 aagaagcaaa attttattac ttataaaata actaataaaa aatattgacc agggaattag
     9541 tagtattact cccaaaattt tcaggttatt ttgtgtctga gtgacaatca tattcaaact
     9601 ggccgttaat tcgttaatca tgattttacc gttgcatctt gtgttgattt gtttgatttt
     9661 ttatctggta taactggtgt aactgtgatt ttgcgaaacg gtacaaaaag taaaggttca
     9721 accaaagttt ggttaactac catagtccag tggagatggg ggcctgttac tcttcctgtc
     9781 ataccaacta atccaagctc ttggccttgt ttgactgttt ctcctgtttt aaccagaatt
     9841 ttgctaagat gagcataaac cgagaatact cccatgccat gatcaaggat aaccgtattg
     9901 cctgtgaaaa aataatcacc ggtatctgct acaactccct gatttacagc atacacaggt
     9961 ttcccctcgt tagcggcgat gtccaatcca gaatgcggat ctcttggctg tttattataa
    10021 actctcttca ggccaaataa actggttata ggtcctctca gtggtgctgt gaattgtttc
    10081 tcaaatggat tggtattgga gtaattagca aaaatttggg ttaatttttt ggtttctttt
    10141 tcaatccgga ttaaatcttc gggttgagga tctactttac tgatatcttt tatatttaaa
    10201 aattgtgtac tgtaaaattt ttcactaaca tgaaagggaa tagatgcttt gatgggtttt
    10261 gtcacgttga tgtattgaac agagttggta tttttaagcg ggattgcaac gattaataac
    10321 cattgtcggg gttgggtact gggaagaacc ggtatgcgtt tcccctcgaa ataggcttca
    10381 ggcttctgat tgaaatcaat aggaataata gtcagtcccc cattgacaga atgattttct
    10441 ggtagagcca ggccatagga tagttttgct gagcttatta atatcaatag gcaggcaact
    10501 gaatgtaata gtttcttcat aattttagtc aatccttaat ttgagtaacc tcacaagcaa
    10561 gacttccttt tgctaaacgg atattgatag tatctccaat ttgtgcctgt tgggcagcaa
    10621 agagtattct ttggttttta ctcacaatag cataacctct atctagtgta gccaaggggc
    10681 ttacagcatg caaagtggaa agattagtgg atagttgatt ttttaattgg tttactttta
    10741 tccggatctc ttgggttaat tgcattatta attgttgtag ttggatttta gttcgttcaa
    10801 tttgtatttt tggattgcta gcttgcaatt gtgttgaaaa aatattcacc ttgttaatgt
    10861 ttaagttaat aaattgagtc atagcagata ttaactgtct ctccagataa tccagagttt
    10921 gccagtatgt tgagatagct tgcctgggtg aggcaatttt atctatcaaa tgattgagct
    10981 ttaattgcaa ccctttaatt agtctgatga tggcatcatg caatcgataa atagccgtat
    11041 caagaatatt gaataattcg atgcagtttg gagtaacagc cgtagcggca gcagtaggcg
    11101 tttccgcacg ataatctgct acaaaatctg caattgtgaa atctgtttca tggccaatac
    11161 cagaaacaac aggaatttcg ctgattgcaa tttgtcgagc aaggtattca tcattaaagg
    11221 cccacaagtc ttcaatgctg ccaccgccgc gtgccaggat gagaacctgg catcgtttat
    11281 gggcattagc cagttttaag gaattgacta gttgttgagg ggcggtttgc ccttgaacct
    11341 cacttggata aataataatt ctggctattg ggaatcgacg gtccagagtc gagagaatgt
    11401 cctggatagc tgctccggta ggtgatgtaa tgatgccaat cgtttctggt attctgggca
    11461 gtgttttttt tctctcagga ttgaataatc cttcgctggc cagtttgatt tttaactctt
    11521 caaatcgttg atagagaatc cccattcctg cttcaactat ctcttcgaca ataagctgat
    11581 attctccccg ggcttcatac agacttaatt tgccagtggc aacaatttgt tgaccatcat
    11641 taaaatttct aagcagagaa ttggaatgtc gatttttaaa aaaagcgcaa cgaatttgcg
    11701 ctgtgctgtc tttcagggtg aaatagtaat gtcctgaaga aggtttgctt aagttagaga
    11761 tttcgccttc gacatgcaca aggcctattt cattctcgag aaaacctttc acttgtctat
    11821 tcagctgact cacggtcagt attggcaatt gactactcat ggtggtaaaa atcccatcaa
    11881 atcaaaaggt tgttaacaac aataactaat taaagaaata aaattatatt gtagtgagat
    11941 gttatttcat ttaaggttat acgttactat atagataatt aacaaaataa caataataag
    12001 gaatgaggaa tgagaaaggc gctgtttgta gttgcaatgc ttacttcttc ctacaccact
    12061 gcagatactc tttatgaagc agtacaatat ggcatgatcg ctaacccgga tatactactt
    12121 aatactgcaa aaggtttgtc agcgaaacag gcaattgata aagcaaaggg tggtttatat
    12181 ccttctatcg atgtaactgg tggttttgga cgacaaagga gtgttaaccc gacatcagca
    12241 gcgatcgatg atactcccag cactaccctc aatatagtgg aatcggccgt ggaattaagg
    12301 cagcgattgt ttgctggtgg agggataatt aatgaagtaa agcgaaacca ataccttact
    12361 gaggcgcaaa agtggaaaac acagggtatt gctgaagatt tggccttgga aattaccaaa
    12421 aattatttcg ctgtattact gcatgaacgt ttgtatgctt actccatcga aaatcttaaa
    12481 gcacataaag cagtttttaa gatgataaaa gaacgggctg atgccgggat taccaggacg
    12541 gcagaagtcg atcaggctaa tgcacgactg gctctcgctg aggctaataa aataagtgca
    12601 ttggctgatt tgcaagaggt gaagatcaat tatgcaaaaa cagtaggtaa atggccagag
    12661 aatcttcaaa tgcctcatgt cccagcaaga aaaagtttac ctaataattt agccaggatt
    12721 atagaaaaag gattggataa tcacccaaca gtaaaatcaa gttatgctga tatcaaagaa
    12781 gcaaaggctc agtacgatgt cgccagagca gcctattacc ccgaggttaa tctggttttg
    12841 aatagttcaa aaaacaagaa cttaggtggt ttgataggtc ctaatgactc cgacacagtg
    12901 gctgtaagaa tgaattacaa tgcatttcgt ggtggtgccg atgccgctcg tatacgagaa
    12961 acggcttatc aagttcagga agcttatgaa acaaaaaatc gaacattgct ggaattaaaa
    13021 gagacaatca ggttagcctg gaatgcctat gtagcctctg ccttgcgcat ccaacccttg
    13081 aagcaacacg ttacagcttc gagaaaaaca cgtactgcct atcaagatga atttaaggtg
    13141 ggtaaaagaa cgcttcttga tttactcgat tctcaaaatg aatattatca atcacaaatt
    13201 gaattggccc gcgcagaaaa tgacgagata ctttcccgct accgaatcct gaatggaatg
    13261 ggttgtttat taaaatattt gaatttgagg ttgccggtaa atgtagttaa taatgatgta
    13321 ttctcgtcgg ctcagaccaa tattttattg aacaggaaaa tggatgagat cccttatccc
    13381 aataatacgg ataatcctat ggttctcact catcctgtta agaacatgga gaaaactcgt
    13441 ttaaccaagt cgatggtaga caaaaacact acctatcctc agcaaataac acccaagtta
    13501 tggtatatct cagcaggctc tttcagagat agaaataatg ctatagcttt ggttaaacgg
    13561 ttaaatggat taggctttac tgccttcatg agcccttata aagatcttct gtccgttttt
    13621 gttggtcctt atgaatacag aggtcatgcg ggtaatggaa tggaaaggct aaaggaattg
    13681 gctcatgttg aaggagtcct ggttactttc aaagagccgc ccaaaaaggc ttagtgttat
    13741 agccaagaga ttccagaatg ttcttttgga atctcttcgt tatagattgg ttttatccat
    13801 ttggactggt ttgatacttt gctttataac aagtttttcc actcaagccg cgcctttaat
    13861 cagtgtggaa aaaattcaaa aattggcgca atcctatcag ggggataccc ggaaacggtt
    13921 taccgcatgg gcgaatttaa ttgaatcgct taaaaaaaag ccagtaaaaa tacaactgga
    13981 aaaagtaaac tctttcttta atcaatttaa ttatgaaact gacccaataa ctggcgcaag
    14041 tgatgattat tggaaatcac cggtagaatt tattgttgat gggggaggtg attgtgaaga
    14101 ttttgccata ataaaatact ttacattagt cgctgttggt gtgccttctg accagttaag
    14161 aatcacttat gctgcttctc ttactctaaa ccaggctcat atggtgctgt ctttttaccc
    14221 aactcctgaa tccgaaccac taatccttga cagtttggaa agtaaaattc tgaaagcctc
    14281 tgcaaggcct gatttgaaac cagtttatag ctttaatgcg gaaggtttat ggcttgcgaa
    14341 aacaagggga gagagttcat tgatggggga ttcaaaaagt ttgggcaagt gggatgctct
    14401 aatgaaacgt atggaataaa aaaggtgata gaccatgaca ttaactaaga aaatggctgt
    14461 aggcgtgttg attatgttgt tattcatttt tatcggaact tatttcatca ctatgaataa
    14521 tgcgcgtaat ttttttatcc aacaacttga aagcaatgct caagatactg cgacatcatt
    14581 aggcttgtct ctatctcagt ctttaatcaa tcatgatgta cctaccatgg attctatggt
    14641 taaagctgtt tttgacaggg gatatttttc atcaattaag gtaaaagaca taaaagggaa
    14701 agtgatcatt ttaaagaaac aattgccaca agaaagcgat ataccacaat ggtttgtgaa
    14761 cttgataaaa tggccatcaa ctgagaagtc atccttaatt atggatgggt ggatgcaagc
    14821 aggagttgtt ttagtcgcta gtgatccgag ctatgtttac gcctctttgt ggcgtaatgc
    14881 tgtagaaatg gtaaacgctt atctgatttt tgcgttagtt gctttggttc taagctattg
    14941 ctttataaaa tatttattgc aacctttaaa acgagtcaca gcacaggctt tggcaatttc
    15001 agaacatgag tttcctgtag aaacaaagat tcctaaaacc cctgaattga ggcaagttac
    15061 cttggctatg aatcaaatgg ttactaaagt caaatcctta tttcaagacc agttaaaaca
    15121 aacagaatca cttagaacac aggtttacca agattcatta acagggatga gtaatcgtcg
    15181 ttatttttta cagcatttgg ctctgatcct ggataatgaa gatgaattta tacctggtta
    15241 tgtcatgatg ctggtcatag atggtctgga tgagttaaat cagaagcaag ggtatcagca
    15301 aggggatcaa ttggtattaa ctgtagccaa aatatgtaaa agctattgga agcaatcgtc
    15361 tgttagtaca ttggcaagaa ttaacgggac gacctttgcc ttgattagtc atgaaaggga
    15421 tcctttggtg tttgaaaagg aatgtcagga gtttgaacaa attttgagcc aaggtatcaa
    15481 tgatattaaa atttgcaaaa tacacatggg ggctgcgagt tattttctcc accaacctgt
    15541 ctccaatctg ttgtctacgg tagatcaagc ggttaaaaaa gctcgtgaaa ctggcgtatt
    15601 ttattgccag aaggagcatg atacttataa atatccccag ttaatcagcg gcgatgaaat
    15661 tagaaactca ttgcaacaga aaaaaataag cttgtatgcg caggcagtta cagacggtaa
    15721 gaattgtttt cataaagagg tcttcgttcg aattcgcaac caggaagggg aggagttagg
    15781 cgctggttat tttataccag ttgctgagaa attaggattg gcctatctta ttgatcagta
    15841 tgtgctcaat gaattaaccg tcatggacat tgcaacacat acccattttg ctcttaatat
    15901 ttcagaagat actttagcaa ataaagtcaa tagcactggt tatttgcgtc aattggaaga
    15961 cactcctgca gctgtcttga gaaatctttc actggaaatt aatgaggctc acgttttgtc
    16021 acatttttca aactccaagt tctttatcaa gcaagccaaa aaattggggg tgacagttgg
    16081 aatagatcgt gtgggtatta aattttctcc gttgcattat ttgagtgatt taaacataga
    16141 ttatttaaag ctgcatggga gtctggtgac agatattgat gaaaatgaaa gcaagcagtt
    16201 tttcatacat tattttaatg aaatggctaa aacgatggat attgcggtgg tagcaactca
    16261 agtggaaagc gaggctcaat ggcaagcatt acaaatagtc cacatacctt gggggcaggg
    16321 acgcttttta tcttctgtag aattaataaa ataacgagat gaaaaacaga ttattctcaa
    16381 tgcattaaaa tgaattcgtg aatagcccta aacccgccag gtgcctctgt aaaatggtac
    16441 aagaataagg tatgataaaa aatcaaataa ttacaataat aatattaata tgaactattt
    16501 tggcaatctg caaatatgtt tttccaaatc atttttattg ttgttgattg ttagtagttt
    16561 aacagcttgt gtggatttga cgggcgcgcg aaatacttat atcaagaaag aaaagcaacc
    16621 tgcaatttct gatgtcccta acaaaaagtc caaagcacaa aatatgtcat caaaaaaagg
    16681 cgaagtccat actatgcttg gcggcctggg aattttcagc aaggggatgt acacacttcg
    16741 tgataaagtg tctgaaaaat accatatcac ggctcatagc accatgtggt ataacgctgg
    16801 agcggtaagt cggtatatta ttgaccatta ctatcaagag aaagatcaca agccaattat
    16861 tttagtgggg cattctttag gggctaatga acaaatcaaa gtggctcgaa atttgaatgc
    16921 cgcaggtgtc cctgttgctt tgttagtgac tgtcgatgca gtatcacaaa ctattgtgcc
    16981 tcctaatgtt aaacaagctt tgaatgtata taaacctggt tttgtaccga tgtttagcgg
    17041 gttaaaactc agagcagtcg atcctcaatt gacaaagatt gataatatca atgttgattc
    17101 gataaaaggg ataaaagtaa atcattttac aattgacaaa gacgaaattt tacaagcgat
    17161 gataatggat aaagttgata aggtgttagt tgatggaaat aaaaagggtg cttaaggacc
    17221 gtctgtctgc ccgtgtttgt gtgatcggtg ctggtcccag cggtattgcc gccattaaaa
    17281 atttgcaaga acacggcatt actaatgtta ctgtctttga aaaaaacaat caaataggtg
    17341 gtaattgggt ttatgatgag caaaatgagc attccagcgt ttatgagacg actcatatca
    17401 ttagttcaaa acgctggtct gagtttgaag atttccctat gcctgttgat tacccggatt
    17461 atccttcaca ctcccagctt ttaaaatatt ttcagagtta tgtggagcat tttcatttag
    17521 atagatacat tcgttttaac acagtggtac aaaaggtaca tcgacttgat gataatacat
    17581 ggcatgttat ctatgaggat gcccaaggaa ttcatgaagc gtgctatgac tatttgctgg
    17641 tagctaatgg tcatcattgg gatcccttta tgcctgtata tcctggagta tttgacgggg
    17701 agatacttca ttctcaccaa tataaaaaag cttcaatttt taaaggcaag cgagtactgg
    17761 ttgttggcgg gggtaattca gcctgtgatg tcgctgttga aatatcgcgg gttgctcccg
    17821 gaacttgcat tagcatgcgt agagggtatc atattttccc caaatttatt tttggtaagc
    17881 caacggatgt agcagtagct aaaatacaat ggatgccatc ctggctaaga cagaagttca
    17941 tcagcctggt cattagagga ttgcaaggcc ggtacgccaa gtataaatta atgaagcctg
    18001 attgtgggcc attggaaatt caccctacta ttaattccga actcttgtat tttattcgcc
    18061 atggcaaaat acaccctcgt cccggtatat cgagatttga gggtaacacc gttcatttta
    18121 ctgatggaac ccagggggag ttcgatacga tcattttcgc tacgggttac caaatcagtt
    18181 ttccattttt tgataaaagt tgtattgatt ttagtaactc taccaccatc cctttatatc
    18241 gaaaaatgat gcaccccgag tttgaaaatt tgtatttcat tggcctatgc cagcctcaag
    18301 gttgcatttg gcctttggct gattatcaat caaaaatagt ggctcgcatt attgcagggt
    18361 ctttgaaacg acctgatcaa ttacatcgaa aaatagccaa agaaatcaat aaaccccatc
    18421 accgatttaa atctcacatg cgccatgctc tagaggtcga ttatcatatt ttcagaaaag
    18481 aattgttaga aatgctaggc taaaaacata ggagactgaa attctacccc tagattcgta
    18541 atgagtacat gaatgttaaa gcatggatga tcacatcagt tataaaccgg gtaacaatta
    18601 ttctgttttg aaaatcgcaa acaatttaca gattggcttc tgatgattgg cgctttaata
    18661 aagaacacat taattgttcg acgctgttac gatgcggatt ttcctgcaac agtatggctt
    18721 tggttctgtc ttcacaatat cctttaactt ctttatcagg cagtatgtaa aatctttttt
    18781 gttcaacttc ccttactata tgttctgcaa cttcaatggc gggtcgagaa tgtgctaaca
    18841 agttatttaa ttgattgtgg attggagaat ggtttaattt cccactactc gctaataatg
    18901 aggtatctgt aaaagaagga aatactacag aaacatctac aggtttttta agacgattta
    18961 aatcaaaata taatgactcg gataaggcta atacagcatg ttttgacatg gaataagcgg
    19021 ccatttgtga gcctgtacat aaggcataca ggcttgccat gttgataata tgagaacgga
    19081 aattctgttt gaataaataa ggcatgaatg agcgaatcac attaagcatc ccgtgcaaat
    19141 tcacttgcat tacctgatta atatcatttt cttgcagttc ccaggcaggg agtaaattac
    19201 caatgatccc agcattatta aatatccagt ctacctggcc tagttgttta caggaatatt
    19261 ccgcaagtcg ttctacttct gatggtttgg ttacatcgca ggcataacaa ataacatttt
    19321 caggagaaaa tttagtcatg aatgattggg cttctgtatg taatttgtct tgattcttat
    19381 caaccatgac tatagttttt ccattgctaa gacaaacttg acttaaggca tgtccaatgc
    19441 cacttgctgc accagtgata attactacat tcatataaaa taccttacaa aagtttaatg
    19501 ccctcttctt caggacttac gaaaaacccc aatctcttcg ttaaaaaaag tttttaattc
    19561 ggtcatttag tttatctaaa ctccctcatt aaaaactttt tttgccttgg ccttggggtt
    19621 tttcgtaagt cctgtttctg tggcgctagg gaggcattga ggtttctaat gtggatttta
    19681 tgaaaaagtg tatcatgggc aagagtattt accaaaatga ttgctaaaat tgattttgct
    19741 atactttgaa tagaactatc agaaggaggc gatactttga atacaactga aataccagaa
    19801 cagccaattg agtatccaga acagccaatt gagtatccag aggagccatc agagtaccct
    19861 gaacaacctt tggagtaccc tgataatcca gagcctctgg agcctggtta tcccgaatta
    19921 cctgagcctg agccaagtca aactcctgag ccaggttttg agtagtttat ttagtcagtc
    19981 attagtttct gtaatgcttt cccgatgtta tctgccctca ttaaactctc tccaatcaaa
    20041 aaggcattaa tgccatggga ttgcattaat tttatgtctt cacgggtgtt gattccgctt
    20101 tcagtaataa taattttatc tttaggaaca aattgtttta attggatact cagttgaatg
    20161 tctgttttaa agttgtgtag gctgcggtta ttaatgccta taagaggggt gggtaagcgt
    20221 aatgctcttt ctaattcatc ttgcgtgtgg ctttcaacca aaacagacat tttaagttct
    20281 tgagccagtt ggcaaaaatc cattaattga acatcatcta ataatgcaac aatcagtaaa
    20341 atgcaatcag cgcccaagac aaggctttca tagatttgat aagaatcaat aataaagtct
    20401 ttacgcaata cgggcaaagt agtttttgat tttgctaaag ccaaataatc aggatgacct
    20461 tgaaaaaact caatatccgt taaaacagat aagcatcttg caccattttg ggtataaatc
    20521 ttggcaattt ctgcaacatt aaaatctttt cgaattaaac ctttgcttgg agaagccttt
    20581 tttatctcag ctatgactgc aggtgaggca ttggatttta aagcagttat gaagtcgcgt
    20641 atttcacctg gttgttgttt tgacagaaca tgtaaaggct tatttttttt cgcaactgca
    20701 acttcttcta gtttatgttt ggctatgcgt tccaggatac tattcatgat ttgattcttt
    20761 atttaaagtt tgagtcaata atcggagttt gttaaaacaa tgatttgcct taccactatc
    20821 aatggcaatt tttgcttcct ctatggccgc atcaaacgat atgccatctt tggcacaata
    20881 aatggctgcg gctgaattga gaaggactat atctcgagct ggaccagaat cacctgaaag
    20941 tacggattga atcaaatgca ggctttgctc tggtgagtcg actatgattg catctaaaga
    21001 agaatgcttt agaccataat cttcaggaga aatgaaccat tgcttaaaat ttccatctcg
    21061 gtattcaact acttcgcttt tcgctgcaat actaatctca tccagtccat cttgagagct
    21121 tattactaat gatctttcac tacctaagtt ggccaggaca gtagctattg ttttgagcca
    21181 attcgttgaa aatacaccaa cgacttgtcg ttttacctga gccgggttaa tcagtgggcc
    21241 aagtaaatta aataaagttc ttattcctaa ttgttgcctt gcagctctcg catgctgcat
    21301 ggcggggtga taatgtggcg caaacaagaa agccaattga cattggttga tgcagttttg
    21361 tacctgtgag tcagaaagat taagtataaa tccggcttgt tctaatagat cagcgctacc
    21421 gctacgactc gaaacagaac ggttaccatg ctttgcaact tttattcctg ctgcagctac
    21481 aacaaaacta caggctgttg atacgttaaa agtatttctt ccatcaccgc cagtgccaac
    21541 tatatcaatc aggggatttc ccaaatctat tttgtgggct aattgcctca tgactttagc
    21601 tgctgcagta agttcattga ctgtttcacc tttcatgcgc attaatgcca gaaaggttgc
    21661 aatttgcaca tcactaaatt cacctgtcat acaagcatgg atgacctctt gcatctggtc
    21721 tgagcttaaa tcctgtcggg atagaagttg ttcaaataga agtttgggtt tcatagcgta
    21781 aaaaattctc gagtatttgc aagccaaatt gacttaatat ggcttcagga tgaaattgta
    21841 gaccaaaaag aggaaattga cgatgtgata ttgccattat tgtatcgtct gcccacgcat
    21901 ctatcgaaaa gcaattgggc aagctcatga tatccacggc taaagaatga tatctcgtcg
    21961 cttgaaagtg atttggtatt cctgtaaata agccctggta atgatgggtg atttctgagg
    22021 ttttaccatg catgatttct ggtgctggga tgattttgcc tccaaatgcc tgagctatac
    22081 actgatggcc aaggcaaata cccaatatcg gaatatgctt gtgaaactct ttaatgactt
    22141 ctatagagat tcctgattca tcaggagagt tgggtcccgg tgaaatgacc aggtattgcg
    22201 gcgctgattg cctgatagca tcgatttgaa tttcgtcatt tttaaaaact gcaacttttt
    22261 gatttaagct ctggaagtac tgtaccaggt tgtatgtaaa tgaatcgtag ttatcaataa
    22321 ttagtaacat aagtataatt aaaatgtatt cttttaaatg aggctacaaa gcaaattctt
    22381 cacccaaata aacttgcctt acttgttgat tggcaagtat tgctttgggg gttccttcgc
    22441 aaagaatttt tccctgactt acaatataag cgcgttcgca aatgtctaaa gtctctctca
    22501 cgttatgatc tgtaattaaa acccctatac ctttatcaca caaatgcagg ataatttttt
    22561 tgatatcgat tacagaaatt ggatcaaccc cggcaaaagg ctcatcaagc aagataaagg
    22621 atggttctat tgccagggct cttgcaatct caacgcgtct tctttcgccg cccgataagg
    22681 ccatgcccaa gctgttttct atgtgagaaa tatgaaactc ttgtaagaga agatccagct
    22741 tttcttcacg tttttgatca tctaaatctt ttcttagttc cagaatcgcc atgatatttt
    22801 cagcgacagt aagctttcta aaaacggagg cttcttgcgg aaggtaacta ataccatgcc
    22861 ttgctcttag gtgcattgcg tcttgagtta tgtctgttcc gttaagaata atatggcctt
    22921 catcacagga ttgcagacct acaatcatat aaaaacaagt ggttttaccc gccccgttag
    22981 gacctaataa accgacacat tcaccttgct taatgtgaat gctaacacca ttcacaaccg
    23041 tccggccctt aaatgatttt tttaattgtt tagcctctag taaactcatg atttcttttc
    23101 aggataaata atgattgtcg ttcttgtttt ttcgtcacta tgtgataaaa catgttgttc
    23161 ctgagtatca taactaattt ttgccgctga aagagagttg ggtccttgct ctatgcgagc
    23221 attcccaatt aattcgatta aatgttttag cggataatat tttatagtgt cagcataggc
    23281 atggaatggg ggtttatcag gggctgttgt agtccaataa tgagcttgct tgtctttaga
    23341 gccttcagca atggctaaag tgagttgatt tttggcatct ccttgcgtaa tggctttttc
    23401 tgcacgcaaa ttggttgacc cttgaacgaa ctcaacattt cctatataga ctccctgatg
    23461 atttttctga tttaagtcgg ctgaatcaga aactacttgc accatttttt ccttatcaaa
    23521 cggaagagca tttcctgaaa tggagcacaa catcaacaat agcagtataa tgtatttaac
    23581 ctttagctgg gtcataacta cctctggctt tatggagtaa ttctactctt ttttcatcca
    23641 aataggcatt cattccagta gactgtatga tattgcctgg ttgttcaaag gttacaaaaa
    23701 gacttgttgt tgcttttttt tccttgggat aataagtaat ctcttcagtt tttaaagtgc
    23761 tttccctggt gttttctccc ggattttgat gaactactac ttgctctata aaagtaattt
    23821 gtttaccacc atcaaacgat tttgcccgta atgaattgat ttcccacgcg ggttgattat
    23881 cttgcttaat aataatatgg gggtttttaa gcaaatggag attgttgtta ggaatatatt
    23941 ctacctgtgg agtggttaat aagttaatta acacaccttc tgaattaaat tgctttacgg
    24001 taagctgcga aatcgttgtg tcaacttcat ttgacaacgt tttcttattg ataatagcct
    24061 gattaaaatg agcataatac caaccagatc ctgctaaaat aagcaaggtc aagaatagcc
    24121 aaattgcttg cttggtaata ttcattgttt taaatagcct gtgatcgcca attctgcttt
    24181 attttgtgcg ttaagaatta agtcacataa ttcgcgaacc gcgcctctac caccagtccg
    24241 ctctgttctc caatcagcaa attccagaac ttgcggtaca gcattactga ctgcaactcc
    24301 taatcccact tgttgtatca agggtaaatc tggcaaatca tcgccaatat aggcaaactc
    24361 ctcatcattg agtcccaatg ttttttttag atgttggtaa gcgctacgct tatcaacttg
    24421 acctttatag taatgagtta tccctaattg ctccattcga tggtcaacta cagcattttg
    24481 tgctgtggta atgattgcaa cttgtatccc ggcggccatt aataatttta atcccattcc
    24541 atcttgtaca tggaatgatt tcagctcatt gccgtgatta tcaatgtgaa gtaagccatc
    24601 actgagcacc ccgtctacat cacaaataag acatttaatt tttttcgctt tttccaatag
    24661 ttcgttcatt tctatctctg tattaaaaaa cacccgcttt tagcaagtca tgtaaatgga
    24721 gtaccgcata aggtctatta tcattttcaa ctactaccaa ggatgtgatg ctatattttt
    24781 gcatgattgc taaagcttcg gcagccaaca tgcctttaga aatagttctg cagtttttag
    24841 tcatgacgtc cttgattaga gtagtgttga tattaaattg acgtgtcaat gttcgacgga
    24901 tatctccatc agtatataca cccactaaat agccatggtt gtctacaaca caagtcattc
    24961 ccagcttttt atttgtgact tcaattaaag cgtcactcac tgttgcgttt tcattcgcta
    25021 agggaagttg ctctcctgta tgacataaat cgtctatttt tagcaacaat tttttgccaa
    25081 gtgcgcctcc aggatgggaa agagcgaagt cctctgcact aaatccccgt gcttgtaaaa
    25141 gagatatagc caatgcatcc cccataacca gcgataccgt agtactggtt gttggagcaa
    25201 gacccagagg gcaagcctct tgtttaatac tgacatccag attgacatct gcagatttag
    25261 ccagagttga ttgtttattg ccggtcaatg taattaaggg gacttcaagc ctttttaata
    25321 atggaagcaa agtaactatt tcgtgggtat tgcctgaatt ggaaatggca acaactatat
    25381 cctggcgagt tatcataccc aagtctccat gacttgcttc tccaggatgc ataaaaaaag
    25441 cggggctgcc agtgctggaa aaagtagagg ccagtttatt agctatatgg ccagattttc
    25501 ccatgcctgt gacgacgata cgccctttgc atgataagag taattcacaa gctttttcaa
    25561 aatgttcatc aattcgttga gttagctcaa atacagcttg tgcttcagtt tcaataactg
    25621 ctagtccaag tgtgcaaaag ttcataggtc attgagtcgt aattttgttt ggttttcatt
    25681 ctaacataat gtatttaaat gatgtaccct atatttcagt aagtgatacc gactaatggc
//
LOCUS       DATNSN010000028        25655 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.028, whole genome shotgun sequence.
ACCESSION   DATNSN010000028 DATNSN010000000
VERSION     DATNSN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25655
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.028"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(245..1753)
                     /gene="ankG"
                     /locus_tag="VAH76_000996"
     CDS             complement(245..1753)
                     /gene="ankG"
                     /locus_tag="VAH76_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkG/AnkZ/LegA7"
                     /protein_id="HEO1385643.1"
                     /translation="MIYVLICIMLVCGLVVYQLMPKLLSYSTSLISKKQDTSELTHDG
                     ITNVLTQLGHPKFEGVCYGFTLNWALAVAQGKESFFYRQLHHLRTHQFGLPKTLQQIK
                     EKKERNQSLSNDEMVIDTLPQLGKKICVAQDPLQYKEKYRKLVWQPDINSILKAINAD
                     SSVAKHIFYKTHSFLNQDEATEYLELLKRTGIREDVAVIISTADHAMGFKLAGNVWRF
                     ININDLYQQDKNKPYFEFSSRNLVKELYRVCAENLQGSRLTVNTDFVSVNPEEKLSRA
                     LQNVFPVFPVRTKTSYPERLAFFSMAATQGDMNSVKKCIHSGWSIFSRQRLSDDSPIL
                     TAIYLGRRDVVRAMLSTSRHRVNQKRKSDSSTLLHIACRYGGSGIVEDLLNIRGIKID
                     PQDNKGRTPLMYACKKSVVTEDKKLFNLLFAKGASLSIKDNDGLTALDHALKNEHTLA
                     IQMIEERLEKEACAKENSTSRRFKFSETKGTLFQRGVKTISYQSQQPGFGMK"
     gene            2014..2691
                     /gene="legA7"
                     /locus_tag="VAH76_000997"
     CDS             2014..2691
                     /gene="legA7"
                     /locus_tag="VAH76_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegA7"
                     /protein_id="HEO1385644.1"
                     /translation="MESLMPKAFTHLCKKVNLSLYNDTLTVYPKSSALPKPIQIRSEL
                     DLLMVLLFSQMDCNSAQVDQFFMMRSNELGVYRFIHRDDSDHDLYHQDEFSMDDNHYH
                     AQFDKAIDESKLENILGILEKHSLISSEEHVSFIEAYHKANTLPEDMPKLETTLPSPL
                     PVSSPSVHTTKKQPVVKQKEVDPPTTFAGLRVGFFLTSTKTTTKPLSRSHDDDEKKIG
                     TNGHGFH"
     gene            2949..4325
                     /gene="ffh"
                     /locus_tag="VAH76_000998"
     CDS             2949..4325
                     /gene="ffh"
                     /locus_tag="VAH76_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO1385645.1"
                     /translation="MFENLTERLTRTFKNLRGQGRLTEDNIQHALREVRLSLIEADVA
                     LPVIKEFIEQVKQKSLGQEVLTSLNPDQAFIKIVHDELIHVMGDERVELDYKTQPPAV
                     FLMAGLQGSGKTTSTAKLARYLKETENKKIMMASVDVYRPAAIHQLKVLAEQIDVAFF
                     DSDPNEQPIKIAQKALESARKQYVDVLILDTAGRLHVDEDMMSEIKSLHKAVNPIETL
                     FVVDSMTGQDAANTAKAFHEALPLTGVILTKTDGDARGGAALSVKQITGKPIKFIGSG
                     EKIEALEPFHPERIASRILGMGDILTLIEEVERKADKQASEKLAKKLKKGKGFDLEDF
                     KQQLLQMNNMGGISGMMSKLPGMGQLPKQAMQQVNDKAMAQTIAIINSMTPKERRIPK
                     IIVGSRKKRIALGSGTQIQDVNRLLKQFEQMQKMMKKFTKPGGMQKMMRGIGGMAGLK
                     GMFPDDLK"
     gene            4412..4672
                     /gene="rpsP"
                     /locus_tag="VAH76_000999"
     CDS             4412..4672
                     /gene="rpsP"
                     /locus_tag="VAH76_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO1385646.1"
                     /translation="MVVIRLSRAGAKKRPFYHMVVTDSRKRRDGNYIERIGYFNPVAR
                     GQEVKLHIDMDKMTHWQKVGAQLSDRVSALLKEHSKKSETAA"
     gene            4678..5187
                     /gene="rimM"
                     /locus_tag="VAH76_001000"
     CDS             4678..5187
                     /gene="rimM"
                     /locus_tag="VAH76_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO1385647.1"
                     /translation="MNNKTNWVIIGRFGRPHGIKGFVTVHSFTDPADNILRYNDWHVF
                     LNKQWQPLKLLTIEVRSKAIIAQIEGYPERELVSALTNLDIGVQESQLAALAPGEYYW
                     YQLIGMSVINSKGDLFGKVVEIMPTGSNDVLVVEGEKRHLIPYLPGQFVINIDESQQV
                     ITVDWDMNF"
     gene            5187..5939
                     /gene="trmD"
                     /locus_tag="VAH76_001001"
     CDS             5187..5939
                     /gene="trmD"
                     /locus_tag="VAH76_001001"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO1385648.1"
                     /translation="MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSS
                     RPYKQVDDKPYGGGPGMVMMYEPLHAAIKHAHSEMKENCKTIYLSPQGKVVRQNDLKQ
                     IATQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
                     SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKT
                     WLKRPDLLEKIQLSETDKQLLAEFKCEHGDSC"
     gene            5966..6331
                     /gene="rplS"
                     /locus_tag="VAH76_001002"
     CDS             5966..6331
                     /gene="rplS"
                     /locus_tag="VAH76_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO1385649.1"
                     /translation="MTNIIDQINAEQMQGKEIPDFNPGDTVLVQVKVIEGNRERLQAF
                     EGVVIAKRNRGLNSAFTVRKISHNVGVERVFQTYSPIVDSITVKRRGDVRRAKLYYLR
                     NLAGRAARIKEKLSGKKGD"
     gene            6347..6802
                     /locus_tag="VAH76_001003"
     CDS             6347..6802
                     /locus_tag="VAH76_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO1385650.1"
                     /translation="MLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLI
                     ASELKAYFDDPHHRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRA
                     VGQACKRNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII"
     gene            complement(6903..7766)
                     /locus_tag="VAH76_001004"
     CDS             complement(6903..7766)
                     /locus_tag="VAH76_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0393 family guanine-nucleotide exchange
                     effector"
                     /protein_id="HEO1385651.1"
                     /translation="MLSSTKEYLQALRDGKYLLFLQWPKFIAEYYGKNDKNQEADEMV
                     SLLIFEWLNNGFCLDDIKKFAILYAVHEMESRPLREGLSYALTTISIALFPCMVYLTN
                     NLQERYITSKKLSSKEVLQLMTMNNAYLEKQRFVEFLGQEQDKFFTWVKEADSSAVSK
                     AFDQIYSVTYLKYLIEDYLNLLESAHLPNDQLKSSRISLVIRLAKYLNEQTELTQDVH
                     DEIAVYVKKLWEMQPAEFEEEFLKKISPLPFIDNTVRILTGLSARFFSILQPPSPPLN
                     INSDADKNPKV"
     gene            7915..8196
                     /locus_tag="VAH76_001005"
     CDS             7915..8196
                     /locus_tag="VAH76_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385652.1"
                     /translation="MKNKLAMSILLFLLPTAAAMANCDLTKFRWDCDLPVQVKPSPGA
                     SSLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER"
     gene            8381..9088
                     /locus_tag="VAH76_001006"
     CDS             8381..9088
                     /locus_tag="VAH76_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEO1385653.1"
                     /translation="MHIQIDGIPIEILRKPIKNLNLRIYPPDGLVKVSVPLKYSEQLI
                     KQQLQEKSEWIRAQCERIRARSFYREPSLQTGATIPFKGKNYLLIVEEHHGPSQILIK
                     DELIYCYTKPDLTPQQKQNILERWYRKEMNLVIPRLIQQWEAIMKVKINQWGIKKMKT
                     RWGSCNPRAQRIWLNLNLIKKPLECLEYVLVHEMVHILEASHNKRFYALMAQFMPKWQ
                     EYQRILEGTPNANTFAR"
     gene            9174..9812
                     /locus_tag="VAH76_001007"
     CDS             9174..9812
                     /locus_tag="VAH76_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEO1385654.1"
                     /translation="MFNPDLIAHNICTQGFHIIDEFLEEAHYYSLRDMAYELKQQNLF
                     RNAKIGQNLQAQQNDKIRSDQIFWIDADSPYPPIQAYLKKLISLGNVLNQTMYLGLAE
                     FETHFAIYLPGTFYRKHIDQFESTKTRKISFVYYLNDTWQKEFGGELLLYNIEDELIQ
                     NISPLGNRFVCFDSQLPHEVHPTQRTRFSMTGWMKTRSTSVLADNKLTQITQ"
     gene            9899..10957
                     /gene="vipA"
                     /locus_tag="VAH76_001008"
     CDS             9899..10957
                     /gene="vipA"
                     /locus_tag="VAH76_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipA"
                     /protein_id="HEO1385655.1"
                     /translation="MKHYDSMDQELITMPISNAFLKLFSEFVDNYARNGTPSFYHWAK
                     EQNHEVINKFFHELLEIIKSNTETFHDVSIIEHLNATVLLSAKTNPYLLDCWISFITK
                     YNAETNSFTPIGASMHIKIGPEGTPSERIIRELKESLVMTQQAFILAQKANATEVDSL
                     KRQLERTKLENERLVTQNQFLKDQVRAEKTFADINPQINNARHQLSALTELIQTLSEI
                     TNAKPKIETVSSLSIPAVTENKSELLKENSCSVAPVSKEVTPAISQEPKKRPVPPPPN
                     DMDSQALFPNIPPKEDVSIKVTIPSMPKPNSTQPVAKNSGFFSGDGFLKELNAKIKER
                     KERPKDEPHTTPVEKKNL"
     gene            11188..12102
                     /locus_tag="VAH76_001009"
     CDS             11188..12102
                     /locus_tag="VAH76_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385656.1"
                     /translation="MHALEINQLSKTYSNGVEALKGIDLTVNTGDFFALLGANGAGKS
                     TTIGLITSLVNKTSGNIKIYGFDLDAYPEKAKSCLGLVPQEVNLNIFENCEQILLNQA
                     GYYGISRKKALPRAEALLEQLGLWDKRRSIVRHLSGGMKRRLMIARALIHQPKLLILD
                     EPTAGVDIEIRHSMWDFLARTNAEGTTIILTTHYLEEAEQLCKNIAIIDKGQIIKNTS
                     MKALLQTLRHQTFIFNTENPIDYLPDIHPFNVTPIDPTTFELRVDNNFSLNEVFAVLT
                     KQGIKIHSMRNKTNRLEELFMDLIKNGI"
     gene            12092..12865
                     /locus_tag="VAH76_001010"
     CDS             12092..12865
                     /locus_tag="VAH76_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385657.1"
                     /translation="MGFKYQLIALYTLVRREVVRMFRISSQVFLPPVITTTLYFLIFG
                     SLIGPRIGDIEGVDYSQFIAPGLIMMSVIINSYGNVSNSLFSVRFQKSIEEMLISPMH
                     NGLLLLGYVIGGVLRGLIVAILVFVISTFFLPVELDHLPMTLLVVVLVAAIFSLAGFT
                     NAMVARNFDDIMLIPTFILTPLTYLGGVFYSINMLPPFWQKISLFNPILYMVNALRHT
                     MIDQEEVSITLAMIIIFLMLIALTVGNMILLKKGVGFRE"
     gene            complement(12892..13911)
                     /gene="htpX"
                     /locus_tag="VAH76_001011"
     CDS             complement(12892..13911)
                     /gene="htpX"
                     /locus_tag="VAH76_001011"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO1385658.1"
                     /translation="MGIADYHATAGDWRAQLRRNEKKTRRVIAVFFLVYILVGLIVDA
                     FVLQGMYPHASLENIIRALITFKVVPIATLLMIAFAGISLLITYALYDRIMLLGTEYY
                     QITPETARSLQEKQLYNVVEEMKVASGLQYMPKVYIIEASYMNAFASGYSEKSAMVAI
                     TRGLMEKLDRAEMQAVMAHELSHIRHHDIKLTLMVAVLSNILLIVIDILFYSVIYKRD
                     RRGDNRLLMVIIILRYVLPIITVLLALFLSRTREYMADAGCVELMRDNEPMARALLKI
                     NQDHVQNAEQYAKAYGDTPHEQVRQASYLFDPSHIDPVKSLNNAFSTHPSIEQRLEAL
                     GFKRK"
     gene            complement(13922..14503)
                     /locus_tag="VAH76_001012"
     CDS             complement(13922..14503)
                     /locus_tag="VAH76_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO1385659.1"
                     /translation="MKTFFIAILIIIALAFIWAISIYNTLIGLIEAINNNKKQIDIQL
                     DRRFKVFESLIEAVKKYMDYEKTTLKDVVALRNQAQAAKEAGDEQGRIAAENGISKIA
                     SNLNLVFEQYPDLKASQNVMQLQEEIVNTENKLAYAKQAYNDGIERYNAKKKSFFESM
                     VVAMFSSALDKEFIYWGLPEEQIQAKENYTVKL"
     gene            complement(14687..17542)
                     /gene="uvrA"
                     /locus_tag="VAH76_001013"
     CDS             complement(14687..17542)
                     /gene="uvrA"
                     /locus_tag="VAH76_001013"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO1385660.1"
                     /translation="MHTITIRGARTHNLKNLDLELPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDSIEGLSPAISIEQKATSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPTHHTSLHAQTISQMVDQVLALPEDSKVMILAPVIRERKG
                     EHVQFLQQLQAQGYVRARIDGELYELDSPPKLGLRTKHTIEVVIDRFKVRPDLAQRLS
                     ESFENALNLADGLAIVSSMDGHFKELLFSSRFACSECGYSLSELEPRLFSFNNPVGAC
                     SSCDGLGVDQFFDPSRVVHDATASLAEGAIRGWDRKTTYYFSMLESLARHYGFDTSTP
                     FCDLSEAMQQIVLYGSGREIIEFQYHRPHGGYMVKRHSFEGIIPNMQRRYRESDSGMI
                     REELAKYLSSRPCQSCQGARLREEARHVFIDNKNLPEITSYSIEQAYQFFKELKLSGY
                     RGEIAAKINKEIVERLGFLVNVGLDYLSLARSAETLSGGEAQRIRLASQIGSGLVGVM
                     YILDEPSIGLHQRDNDRLLSTLMHLRNQGNTVIVVEHDEDAIRSADFVLDIGPGAGVH
                     GGEVVASGSPEEVMNNPASLTGQYLSGKKEIEIPKDRIPVNHDRMIHLKGVSCNNLQN
                     VDISIPLGLLTCITGVSGSGKSSLINDTLYPIAANALNRASLMTIGEVKDISGLHLCD
                     KVIDIDQSPIGRTPRSNPATYTGLFTHIRDLFSGTPESRARGYQPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDIYVSCDVCQGKRYNRETLEIHYKGKNIHEVLDMTVEDACAF
                     FAAIPVIARKCQTLMDVGLSYIKLGQSATTLSGGEAQRIKLARELSKRDTGNTLYILD
                     EPTTGLHFHDTKQLLTVLTRLREQGNTIVIIEHNLDVIKTADWIIDLGPEGGSKGGKI
                     IATGTPEMVAENPQSFTGQFLKQILSKSNEFLLQKCK"
     gene            17637..18095
                     /locus_tag="VAH76_001014"
     CDS             17637..18095
                     /locus_tag="VAH76_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385661.1"
                     /translation="MKSLIKNLLFIVVFSINSSALYATNGNTRVIQWAQQTLMETLSV
                     SYTSKPQDFVTVRKNYSLNAWSGITGFLGGNMQSIREKQLTLHPVLLPDTQILSTGLY
                     SGMSYWIIRQSVAIDELNTKLTFTLTILGRSGAAGSPYIVQSLSILLNAL"
     gene            18354..18905
                     /locus_tag="VAH76_001015"
     CDS             18354..18905
                     /locus_tag="VAH76_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEO1385662.1"
                     /translation="MPKCMHFILATALIFVTSTFYASNLREIEQEVSDSVITAKITAK
                     FAESRHLNPLKISIDTEKGIVTLSGYAPDKQAFIDALQIAKNTKGVKAVHAKDLHIKK
                     VNTAFTDAFITAEVETAVLKAKILDDESIPLVGINASTTNGEVTLSGHVKSARSIAAI
                     IKRVNEIPGVKKIVTNLEIQENT"
     gene            18902..19375
                     /locus_tag="VAH76_001016"
     CDS             18902..19375
                     /locus_tag="VAH76_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEO1385663.1"
                     /translation="MSPLTQFKFFLWFFSHFKVALIGYLKPKLIQLTDNEIVVRLPLT
                     RRSRNHLHSMYFGALAVGADIAGGLHGFYHAKQAKCKVSLAFKSFQAQFLRRPESDVY
                     FICTEGDVVKKMIEESKKSGERINKPIHIKAYINYLTHPEEIADFILELSLKVIK"
     gene            19570..20283
                     /locus_tag="VAH76_001017"
     CDS             19570..20283
                     /locus_tag="VAH76_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEO1385664.1"
                     /translation="MIDLIIQTQPDDETCGPTSLHAIYLYYGLDLPLNEIIQGVDKSL
                     SGGTLTPMLGKHALLHGFRTTVYIYNLNIFDLTWFHNGEVNNSFLMDKLEAQMKYKND
                     PYITKASMAYLDYLKLGGTIRSKSLNVDLLKNYFNKKIPILTGLSATYLYRSAREYFT
                     PEGKSVYDDIRGEPCGHFVVLCGYDVKKRLVIVADPHAENPLSHNNYYKVSINRLINS
                     IMLGVLTYDANLLIIEPKE"
     gene            20287..21741
                     /locus_tag="VAH76_001018"
     CDS             20287..21741
                     /locus_tag="VAH76_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HEO1385665.1"
                     /translation="MQTVIVTDDLDSWSFLSELAPIVHALEYLSGDEYHQNKSFRVIN
                     LCRSYNYQSIGYYVSLLAQARDHKALPSVHSIQDVLNVSLSKLISQDTHEEIQHSLHD
                     IKGNEFVLSLYFGQNLAKRYATLAKQLHGLFPLPLIRFYLEKKKQWRIKTVVPLSLSD
                     VPEHHLEFMRQSAENYLSKKRVHQWRKKQRFHDLAILVDPSEPNAPSNKKALELFVSC
                     GEELGLNVDIIDKNEAKAIAEYDALFIRATTSVNHYTYRVSRRAAQENLVVIDDPQSI
                     IKCSNKVYLAELLSSHQIMTPDTLFISKYDKELPKIEFPCVLKKPDSAFSHGVVKIDD
                     EKSLQKSLNQFFKNSDLVVVQPFIPTEFDWRIGILDNKALFACRYFMAKGHWQIYDWH
                     CQEENKEGDSETLPLHEVPEQIIKIALKSTRLIGDGLYGVDIKSHGNKYYVIEVNDNP
                     NIDHGLEDKILGQNLYEQIMSVFLQRIRRKHGYV"
     gene            21734..22954
                     /locus_tag="VAH76_001019"
     CDS             21734..22954
                     /locus_tag="VAH76_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEO1385666.1"
                     /translation="MSDFPIFSVLGIEIEYMLVDKESLNVQPKSDLILRTLAGHQVNE
                     IELGDIAISNELVLHVLELKNNGPKPLHVPVAEHFQKTIRELQPLLAQHNLLLLPTGA
                     HPWMDPNTETIRWPHGNHDIYNQFNSIFNCQGHGWANLQSMHVNLPYSNEEEFCQLHN
                     AIRLILPLLPAIAASTPFLDGQATGLLDSRLYFYSRNQQRIPSISGDIIPEFIRTERE
                     YQEQILEPMYRDISPYDPKGILQYQWLNSRAAIPKFDMKAIEIRILDSQECVEADIAI
                     AKAINAILKYWQSTSNFYLDNPCDTKSLKYVFDETIKNGLSCHIDNKKLLEQWQLPLQ
                     GMSVNTIWSLLIERISHELDRKQQLALELILKQGNLSERILKACSSSINHDSLKQVYT
                     QLAHCLLRNEQFTP"
     gene            22935..23678
                     /locus_tag="VAH76_001020"
     CDS             22935..23678
                     /locus_tag="VAH76_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEO1385667.1"
                     /translation="MNNLPPDIMKNIALLISCEHAVNTVPYEYQTLFEPHKDVLESHR
                     GIDFGALEIAQSIHKNNHCSLFQATCTRLLVDCNRSINHPHCFSEITKNLSSDKKKKI
                     LDEFYLPFRNRVMDHIHQLISQGLQVWHLSTHSFTPVMNNIIRTTDIGLLYDPRSNSE
                     KILARQWQKEIKILHPQLKVRMNYPYKGISDGFTTSLRKKYSHHEYIGIELEVNQKLV
                     FHNHSLDTLKNTLSLSVSNTIKAYQKSER"
     gene            23841..>25655
                     /gene="sdhA"
                     /locus_tag="VAH76_001021"
     CDS             23841..>25655
                     /gene="sdhA"
                     /locus_tag="VAH76_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HEO1385668.1"
                     /translation="MISEKIKLLESFKQNQFSKLIEKINSKILATLKSGHFNPRLAPY
                     LEKMNKDGVPFTDFDLDPENISQIKKLINALYHARLAFLDLENIDIGNIKRTIPDLKL
                     LYSNTIHQAYQAGYLITNLDVDLQEMFKEEIDLILPVIGKLQAFAGEHQECTKHLAES
                     LKDFPISYKAGEVTGIALEQMQPRTGDWDYQFLTQFSAVLPGYIEKLTQYIQQYSSQI
                     KEKEPTLNNEKLEELQNASLKLLNDLEHLKGNDFFISLKVLKYIHIIRNIITLATSSV
                     EQMGHFSDSSQDVIRDNLAQLKYVVLPTLFGLVDKIEDNAMLKPGTLSIPLMEKIKPL
                     YQLLIYYASKPVNFKEKGEELLSIEDSRFLALRLERTYQRIDEANKALFKIQKAQEAL
                     DNFYKILDTPPYNKQPIHQLPKEIKNQLITYYKIISPYMMDVNPDLNILLIDSFQGPE
                     SWSSYLKKPWRWLRGTLPADHASFVLAQKEALQTLISKKKATQQFHIELNRDLIESVH
                     KQTNLVLFPYSEKTNVFLLDETKALNPANGATDKLKFRPEKGHNILVNPEDLTSEQAL
                     DLYQWYRNKREKFRVASKAYNEFITLLKKQTKTIAETEG"
BASE COUNT         8402 a         5142 c         4642 g         7469 t
ORIGIN      
        1 aatatacccc ttagtatact gacctataaa attgacccaa caggcattga gaattttcat
       61 ttcagtacag gtccgatgcg aaaacatcat atgatgttat atcattaaaa accagtattc
      121 tgtatataaa attaataaca agaatctttc taatgcacat taagtggttt gtttactaac
      181 agggcgcaaa accccaaggt cgagacaaaa agcatgaggt gctaaaaaga ctaattttat
      241 tctcctattt cataccaaaa ccaggttgct gtgattgata agaaatagtt ttcactcctc
      301 tttggaacaa agtacctttt gtctcagaaa atttaaatcg acgagaggtt gagttctcct
      361 tcgcacaagc ttctttttcc aatctctcct cgatcatttg gattgcaagg gtatgctcgt
      421 ttttaagagc atgatcaagt gcggtcaaac cgtcattatc tttaatagaa agagaagcgc
      481 cttttgcaaa taacaaatta aataattttt tatcctccgt cactacagat tttttacagg
      541 cgtacatgag cggcgttctc cccttgttgt cttggggatc aattttaatt ccacgtatgt
      601 tcaacagatc ttcgactata ccactaccac cataccgaca ggcaatatgg agcaaagtag
      661 aagaatcgct tttacgcttt tggttaactc tatgtcgaga agtactcagc atggcacgta
      721 ctacatctct tcttcccaaa tagatggcgg taaggatagg cgaatcatca cttagccgtt
      781 gtcgagaaaa aattgaccag ccagaatgaa tgcatttttt tacagaattc atatcaccct
      841 gagtagctgc catggaaaaa aaagccaatc gttcagggta agaagttttg gttctgactg
      901 gaaacacagg gaatacattc tgcagtgctc ttgacagttt ttcttcagga ttaactgaaa
      961 caaaatcggt attcacggtc aatcgacttc cctgaagatt ttcggcacaa acacgataca
     1021 actctttaac caggtttcgg gacgagaact caaagtaagg tttatttttg tcttgttggt
     1081 ataaatcatt gatgttaata aatcgccaga cattaccagc cagtttaaat cccatggcgt
     1141 gatctgcggt actgataatt actgcaacat cctctctgat gcctgttctt tttaataatt
     1201 caagatactc tgttgcttca tcctggttta aaaaactatg cgtcttataa aaaatgtgtt
     1261 ttgcaacgga agaatcggca tttatggctt tcaaaatgga attaatatca ggctgccata
     1321 ccagttttct gtatttctct ttatattgca atgggtcttg agcaacacaa attttctttc
     1381 ctaactgagg caaagtgtca ataaccatct catcgttgct taatgattgg ttcctttctt
     1441 tcttttcttt aatttgttgt aatgttttag gcaaaccaaa ctggtgggtt cgcaaatgat
     1501 gcaattggcg gtaaaaaaag gattctttac cctgagccac tgccaatgcc caattgagag
     1561 tgaagccata acagactcct tcaaacttgg ggtgacctaa ttgtgttaag acattcgtaa
     1621 taccatcatg tgttaattca ctggtgtcct gttttttgga gatcaaggaa gtagaatacg
     1681 aaagaagctt gggcatgagt tgatacacga ccaaaccgca aactagcata atacaaatta
     1741 aaacataaat catatccctt gaacctgatg ctatccataa tgacttgagt ataaaattaa
     1801 accatcatac aagtcgaggg tataagatat tcgattagct tcaagatcct ccaaacaagt
     1861 caggcttact gctctattat ccctctattg caccgcatga tacctctctg atgccccatc
     1921 atgaaaacat ttaattcttc ttaagaaaac cttaatgttg atgttttaaa ctataggacc
     1981 aaaatataga caactgcttt ttttaaaaaa tcattggagt cccttatgcc taaagcattt
     2041 acccatttgt gtaaaaaagt caatctcagt ttatataacg atacattgac tgtctaccct
     2101 aaatcatctg cactgccaaa acctattcaa atacgtagtg agcttgatct tcttatggtt
     2161 ctgttgtttt ctcaaatgga ttgcaatagc gcacaggttg atcaattttt tatgatgcgt
     2221 tcgaatgaac ttggtgtcta tcgctttata catcgtgacg actccgatca tgatttgtat
     2281 catcaagatg aatttagcat ggacgataac cattatcatg ctcaatttga caaggctatt
     2341 gacgagagta aattggaaaa tattctggga atactagaaa agcattctct tatttcgtct
     2401 gaagaacacg taagctttat cgaagcctac cataaagcca atactctacc cgaagatatg
     2461 ccaaaactgg aaacaactct gcccagtcct ttacctgtat cctctccttc ggttcacaca
     2521 actaaaaagc aacctgttgt gaaacaaaaa gaagtagatc ctccaacaac tttcgcagga
     2581 ttaagagtag gatttttcct tacatcgaca aaaacaacaa caaaaccctt atccagatct
     2641 catgatgatg atgagaagaa aatcggaacg aatgggcatg ggttccatta attgatatag
     2701 tttgaaaccg atagtaaaga tcgttctatt ggtttactta atatgacaat ttgtggtgat
     2761 ttacccaaag tctaaaaact gtagagtaac gaataataaa ttactctaca gcttctaatt
     2821 acaaaatcat aaagataacc cttattgatt cactgttatc aattactgtg actcattaat
     2881 atgtttttct ctgtggtata cttttaactc cctatgttat agtagttatt ttttaggtac
     2941 tacttattat gtttgaaaat ttaaccgaac gcttaacccg gacctttaaa aatttgcggg
     3001 gtcaaggccg cctgacagaa gacaatatcc agcatgcctt gcgtgaagta agactgtctc
     3061 ttattgaagc ggacgtggct ttgcctgtta tcaaagagtt tattgagcaa gtaaagcaaa
     3121 agtctcttgg gcaggaagtc ttaaccagtt taaatcccga tcaggcattc attaaaatcg
     3181 tgcatgatga actcattcac gtcatgggcg atgagcgggt tgaactggat tacaaaaccc
     3241 aaccgcctgc tgtcttttta atggccggct tacaaggttc tggtaaaaca acgagcacag
     3301 caaaactcgc gcgttatttg aaagaaaccg aaaataaaaa aatcatgatg gcaagcgttg
     3361 acgtatacag accagccgcc atacaccaac tgaaagtgtt agctgaacaa attgatgtcg
     3421 ccttctttga ttcagaccct aatgagcaac caataaaaat cgctcaaaaa gcattggaaa
     3481 gcgccagaaa acagtatgtt gacgttctca ttctcgatac agcagggcgc ttgcacgttg
     3541 atgaagacat gatgtctgaa atcaagtctt tacataaagc agtcaatcca atcgaaaccc
     3601 tttttgtcgt tgacagcatg acaggacaag acgctgccaa tactgcaaaa gcatttcatg
     3661 aagccctccc tctgactggt gtgattttga ccaaaacgga cggcgatgct cgaggaggag
     3721 cggcgctatc tgtcaaacaa atcacaggga aacctataaa atttataggg agtggtgaaa
     3781 aaatagaagc cctggaacct ttccaccccg aacgaattgc atcaagaata ttggggatgg
     3841 gagatatttt aactctcatt gaagaggtag agcgcaaagc cgacaaacaa gccagcgaaa
     3901 aactggccaa aaaattaaaa aaagggaaag ggtttgactt agaagatttc aaacaacaat
     3961 tattgcaaat gaacaatatg ggggggatct ccgggatgat gagcaaatta cccggaatgg
     4021 gacaattgcc aaagcaagcc atgcaacaag tcaatgacaa agcaatggct caaaccattg
     4081 caatcattaa ctctatgact cccaaagaac gccgtatacc caaaattatt gtaggctcac
     4141 gtaaaaaacg catagcctta ggctctggga ctcaaattca ggatgtcaac agactattaa
     4201 aacaatttga gcagatgcaa aagatgatga aaaaatttac caaaccaggc ggaatgcaga
     4261 aaatgatgag aggaattggt ggcatggccg gattgaaggg catgtttcca gatgatttaa
     4321 aataattcgt aagaattact tcacatgtcg atgtaatttt cgtacaatac acggcatttt
     4381 tatgtaacca ctctaacagt agaggaaaat aatggtcgtt atacgtttat cacgagctgg
     4441 cgctaaaaag cgtccttttt atcacatggt tgttactgac agccgcaaac gtcgcgatgg
     4501 aaactacatt gagcgtattg gttattttaa ccctgtagca cgtggacaag aagtaaagct
     4561 tcacattgat atggataaaa tgacccactg gcaaaaagtt ggggcccagt tatccgatcg
     4621 agtcagtgcc ttgttaaaag aacatagcaa aaaatcagag acagctgctt aaccaaagtg
     4681 aataacaaga caaattgggt tatcatcggc cgttttggcc gaccccatgg tattaaaggc
     4741 tttgtcacgg tacattcctt cacagaccct gctgataata tcttgcggta caatgactgg
     4801 cacgtttttt taaataaaca gtggcagccc cttaagttat tgacaataga agtaagaagt
     4861 aaagcgatta ttgctcaaat cgaagggtac ccagagcgtg aattagtctc tgcattaacc
     4921 aatttagata ttggtgtgca agaatcacag cttgcagctt tggcaccagg tgagtattac
     4981 tggtatcaac tgattggcat gagtgtcata aactccaaag gcgatctttt tggaaaagtt
     5041 gttgaaatca tgcctactgg ctcgaatgat gtacttgttg tggaaggtga aaaaagacat
     5101 ttaattccct atcttccagg acaatttgta atcaatattg atgaaagcca gcaagtaatc
     5161 actgttgatt gggatatgaa tttttagtgg cattacatct tggagtcatc accctgttgc
     5221 ctgaaataat acaaggcatc cattatgggg taaccggccg agctattgaa caaggcttgg
     5281 ttaaaattga ttgctggaat cccagggatt ggtcatcaag accttataag caagtggatg
     5341 acaagcccta tggtggtgga ccaggaatgg tcatgatgta tgagccattg catgctgcaa
     5401 taaagcatgc gcacagtgaa atgaaggaaa actgcaaaac aatttactta agccctcaag
     5461 gcaaggtagt taggcagaac gatttaaaac aaatagctac ccaaaaacaa tccttgctat
     5521 ttgttgcagg gagatatgaa ggaattgatg agcgaatcat taatcaccac atagatgaag
     5581 aatggtcgct aggggatttt gtattaagtg gcggtgaatt ggccgcaatg gtatttattg
     5641 acgcgataat tcgcttgata cctggtagcc ttgggcattt gggttcagca gaacaagatt
     5701 catttatgaa tggattactg gactgcccac attatactcg gccagcaact ataaatggac
     5761 tgggtgtacc tgatgtttta ttaggaggaa accataaaga aatagaacgc tggcgtagga
     5821 aacaatccct gggtaaaacc tggctcaaac gcccggattt acttgagaaa atacaattaa
     5881 gtgaaacaga caagcaattg cttgctgagt ttaaatgcga acacggcgat tcctgttgag
     5941 gataagccaa tttgaggagt agtctatgac taatattatt gaccaaatta atgccgaaca
     6001 aatgcaaggc aaagaaattc ctgatttcaa cccaggcgat actgttctgg tccaagtaaa
     6061 agttattgaa ggtaaccgtg agcgtttaca ggcatttgaa ggcgttgtga ttgccaagcg
     6121 taaccgtgga ctaaactctg ctttcactgt acgtaaaatt tcccataacg ttggtgtaga
     6181 gcgcgttttc caaacctaca gtcctattgt tgatagtatt acggtgaaaa gacgtggtga
     6241 cgttcgtcgt gccaaattat attacttaag aaatcttgct ggccgcgcag ctcgtattaa
     6301 agaaaaatta tctgggaaga aaggcgatta attcttgaat ggtaaaatgc tcctcgtgtc
     6361 tatttttaat actcctgtgg gtaacctgga agtaacccat gatgagcatt ttattttcaa
     6421 tgcgtccttc acagacaaat cgggcactcc agcccaaaac caattgtccc atttaattgc
     6481 cagtgaattg aaggcttact tcgacgatcc ccatcaccgt tttcagctcc ctctgaaacc
     6541 tcagggaagc ctcttccagc aaaaggtttg gaatacgttg ttagtgattc ccgttggcag
     6601 aacagttacc tatggagaac ttgctacaaa actgcaaagt agtccaagag cagttggaca
     6661 agcctgtaaa cgcaacccac tggctttatt tgttccctgt catagagtag tagggaagca
     6721 tgatctggga ggatatatgg gaaatccaaa cgccatttct tataaaaaaa atctgctcgc
     6781 gcatgaagca aaaatcatct gatggattaa gtttataata atcaatatta cattgatcag
     6841 aacttacgaa aactccaagg caaaaaatat ttttaacgaa gatactagca agtcgtgtga
     6901 gattaaactt tgggattctt atccgcatca gaattgatat tcaaaggtgg ggaaggaggt
     6961 tgtaatatac taaaaaatct ggcacttaat cccgttaata ttcgtaccgt attatcgata
     7021 aacggcagtg gcgaaatttt ctttaaaaat tcctcttcga actcagcggg ctgcatttcc
     7081 caaagctttt ttacatacac agctatttca tcatggacat cttgagttaa ttctgtttgc
     7141 tcattgagat acttggctaa tcgaataacc aagctgatgc gagaagattt taattgatca
     7201 tttggcaagt gtgcagactc tagcaaatta agataatctt caattagata tttcagatag
     7261 gttacagaat aaatttgatc aaacgcttta gaaaccgcag aagagtctgc ttccttaacc
     7321 caagtaaaaa atttatcttg ctcttgccct aaaaattcga caaaccgttg cttctcaaga
     7381 taagcattgt tcatagtcat taattggagc acttccttac ttgatagttt ttttgaagta
     7441 atatagcgct cttgcaggtt atttgttaaa tacaccatac agggaaaaag agcgatagaa
     7501 attgtagtga gtgcatagga taatccttct cttaaaggcc tggactccat ttcatgaacg
     7561 gcatacaaaa tagcaaattt tttaatgtca tccaaacaaa agccattatt taaccattca
     7621 aatatgagca agctcaccat ttcatcagct tcttgatttt tgtcgttctt accataatat
     7681 tctgcaataa attttggcca ctgcagaaat agaaggtatt ttccatcacg taaagcttga
     7741 agatattcct ttgttgatga aagcatagtt atccattacc aaaatctgat tattcaattt
     7801 ataacattaa caatactaaa aatcttgtgg aaaattattt catgttaaac aacatacgca
     7861 catcaattgt tatcctatac ttaaaataaa acgacaaatc aggtggggaa ttccatgaaa
     7921 aataaattgg caatgagtat attattattc ctgttaccaa cagctgcagc catggctaat
     7981 tgtgatttaa ccaagtttcg atgggattgt gatcttccag tacaggttaa acccagtcca
     8041 ggcgcttcct ctctagtgta ttgtggaagc tcttacggct atatcacaaa gcagcaatat
     8101 gatattctgg ctcgctatca acgcgccagt gtcaatatgg ttttggatat caatggtgaa
     8161 tacattgata gcccgtgtat aggagccgag cgttaaaatt attgcagcaa tgacttaaaa
     8221 taaacaatga gaaaaaaaag gaatattatt atttccagca aatttcttat tcaaaatcag
     8281 aggtctctgg acttgtggct cttcaatgaa ctcaactatt aatatcataa atattacttc
     8341 ttctgtataa tcgaaaacaa atcaataagc atattttgct atgcacattc aaattgatgg
     8401 cattcctata gaaatactga gaaaacccat taaaaattta aatttgagaa tttatcctcc
     8461 cgatgggtta gtcaaagtga gcgtaccttt aaaatatagc gaacagttaa taaaacaaca
     8521 gctgcaggaa aaaagtgaat ggattcgtgc ccaatgcgaa cgaattcgcg cacgctcttt
     8581 ttatcgtgaa ccatcacttc aaacaggcgc aaccattcca ttcaaaggta aaaattattt
     8641 attaattgtt gaagaacatc atggcccctc tcaaatcctg attaaagacg aattaattta
     8701 ttgctataca aaacctgatt taactccaca gcaaaaacag aacattctgg aacgctggta
     8761 tagaaaagaa atgaatcttg tcattcccag gcttattcaa caatgggaag ccataatgaa
     8821 agtcaaaatt aatcaatggg gtataaaaaa aatgaaaacc cgttggggtt cttgtaatcc
     8881 tcgcgcacaa agaatctggc tcaatcttaa tttaatcaaa aagcctttgg agtgccttga
     8941 atacgttctt gttcatgaaa tggtgcatat tttggaagcc agccataaca aacgatttta
     9001 cgcactcatg gcacaattca tgcccaaatg gcaagaatat caaaggatac tggaaggtac
     9061 acctaatgcc aatacgtttg caaggtaatc atcgaattca ttacctcatt atttccaaaa
     9121 aataattctt ggcgtacaat gcattatcac tttgcctgat tggtgtagac aggttgttta
     9181 atccggactt aattgcccat aatatttgta ctcaaggatt tcatatcatt gatgaatttc
     9241 tggaagaggc acactactac tctttacgag acatggctta tgaattaaaa cagcaaaatt
     9301 tattcagaaa tgccaaaatt ggccagaact tacaagcgca gcaaaatgac aagattagaa
     9361 gcgatcaaat tttctggata gatgcagact ccccttatcc acccattcaa gcttacttaa
     9421 aaaaacttat ctctttgggc aatgttctga atcaaaccat gtatcttggt ttagcagagt
     9481 ttgaaactca ttttgcaatt tatctccctg gaacctttta tagaaagcac attgatcaat
     9541 ttgaaagtac aaaaaccagg aaaatatcat ttgtctatta tttgaatgat acttggcaaa
     9601 aagaatttgg tggcgaattg ttactctaca atattgaaga tgagcttatt caaaacattt
     9661 ctccattggg aaatcggttc gtctgttttg atagtcaatt accgcacgaa gttcacccaa
     9721 ctcaacgaac gcgcttcagt atgacaggct ggatgaaaac acgctcaacc tccgtactgg
     9781 ccgacaataa actgacccaa ataactcagt agcactttat ccgctgttct ataaaaaaat
     9841 gaataattat tttttaactt cacaacagct attgaacttt aatttaagtt gatattaaat
     9901 gaaacactat gattccatgg atcaggagtt aattaccatg cctatcagta atgcctttct
     9961 taagttattc agtgaatttg ttgataatta tgcgcgcaat ggcacccctt cattttatca
    10021 ttgggcaaag gaacaaaacc atgaagtcat taacaaattt ttccatgagc ttttggaaat
    10081 tattaaatca aacacagaaa catttcatga tgtcagtatc atagagcacc ttaatgctac
    10141 agtactatta tcagcaaaga ccaatcccta cttactcgat tgttggattt catttattac
    10201 taaatacaat gctgaaacca acagttttac cccaataggg gcttcgatgc acattaaaat
    10261 tgggccagaa ggcacgccat ccgaacggat aataagggag ctcaaagaaa gtctggtcat
    10321 gacgcaacaa gcgtttattt tagcccaaaa ggctaatgct acggaagttg actctttaaa
    10381 aagacaactt gagcgaacaa agctagaaaa cgagcgctta gtcacacaaa atcagtttct
    10441 aaaggatcaa gtcagggcag aaaaaacatt tgcagacatt aacccacaga ttaacaatgc
    10501 ccggcatcaa ctcagtgctt taacagaatt aatacaaact ctaagcgaaa tcacaaatgc
    10561 caaacccaaa atagaaactg tatcttcgtt atccattcca gctgttacag aaaataagtc
    10621 tgaacttcta aaggaaaatt catgctctgt cgcccctgta tccaaggaag ttactcctgc
    10681 aatcagtcaa gaaccgaaaa aaagaccagt tccaccgccc cccaatgata tggatagtca
    10741 agcattattc cccaatatac cccctaaaga agatgtgtct ataaaagtta caataccgtc
    10801 catgcccaaa ccaaattcaa cccagcccgt tgcaaaaaat tcaggcttct tttcaggcga
    10861 tggtttttta aaagaattaa atgcaaaaat taaagaaaga aaggaacgcc caaaagatga
    10921 gccacatacc actcccgtcg aaaaaaaaaa tctctagtta agccaacttc tgttcctcct
    10981 gaatataggg cgctcttagc taaaatggag gaaattagaa atgcagtaac agggaaatat
    11041 cctcatgccg atgactcatc gccagagtca actcctcctt catccccaga attaacccgt
    11101 tggaagtaag ggatgaatta aaatccatag gctactgtag aataaaaatt atggtgtata
    11161 atagcgtttt tacttaagta ataaatcatg catgccttag aaattaacca actttcaaaa
    11221 acctattcga atggagttga agcgttaaaa ggcattgatt taacagtgaa taccggtgat
    11281 ttttttgccc tgttaggagc aaatggcgct ggcaaatcga ctaccattgg tttaatcaca
    11341 tctttagtca ataaaacctc aggaaacatc aaaatttacg gcttcgacct ggatgcttat
    11401 cctgaaaaag ccaaatcttg tctgggactt gtccctcaag aagtcaatct gaatattttt
    11461 gaaaactgcg agcaaatttt actgaatcaa gctggctatt atggcatttc aagaaaaaag
    11521 gctttaccca gagcggaagc cttgctagaa caactaggac tttgggacaa gaggcgttct
    11581 atagtacggc atttatcagg tggaatgaag agaaggctta tgattgccag agcactgatt
    11641 catcaaccca aattactgat tctggatgaa cccaccgccg gtgttgatat agaaataaga
    11701 cacagtatgt gggattttct agcgagaact aatgcagaag gaacaacgat tatcctgacg
    11761 actcattacc tggaagaagc agaacaatta tgtaaaaaca tcgcaataat cgataaaggt
    11821 caaattatta aaaatacctc aatgaaagcc ttattacaaa ccttacgcca tcaaaccttt
    11881 atctttaaca cagaaaaccc tattgattat ttgccggata tacacccgtt caatgtgact
    11941 cctattgatc cgactacttt tgaattgcgt gttgacaata acttcagttt aaatgaagta
    12001 ttcgctgtat taacaaaaca gggaataaag attcacagca tgcgcaataa aaccaaccga
    12061 ttagaagaac tttttatgga tctgattaaa aatgggattt aaatatcaac ttattgccct
    12121 ttataccctg gtaagacgtg aagtggttcg tatgtttcgc atttcgagtc aagtattttt
    12181 gccgccagta atcaccacaa cgttatattt tctaattttt ggcagtttaa ttggacctcg
    12241 tattggagat attgagggcg tggattattc acaattcata gcacccggat taattatgat
    12301 gtccgttatt attaattcct atggaaacgt ctctaattca ctattcagcg taagatttca
    12361 aaaaagtata gaggaaatgc tgattagtcc catgcataat ggacttttat tgttaggtta
    12421 tgtgatagga ggcgtgctga gaggattaat cgtcgcaatt ctggtttttg ttatctctac
    12481 cttttttctg cctgttgaat tagatcattt accaatgacc cttctagtcg tcgtgcttgt
    12541 tgcggccata ttctcgctgg ctggatttac caatgccatg gtcgctcgaa attttgatga
    12601 catcatgttg atacctacat ttatcctgac cccccttact tacttgggag gagtctttta
    12661 tagtattaat atgctgcccc ccttttggca aaaaatctct cttttcaatc caattttata
    12721 catggtaaac gctttaagac acaccatgat cgatcaggaa gaagttagta ttactctggc
    12781 tatgattatt attttcctga tgttaattgc tttgacagta ggaaatatga ttcttttaaa
    12841 aaaaggagtg gggtttcgcg aataaaaaac cgaattccct gcaaaacgga tctatttgcg
    12901 tttaaaaccc aatgcttcca ggcgctgctc aatgctagga tgagttgaaa aggcattatt
    12961 tagcgatttt acaggatcaa tgtgagaagg atcaaaaaga taggaagctt gccttacctg
    13021 ctcatgagga gtatctccat aagcttttgc atattgttcc gcattctgaa catggtcttg
    13081 attaatttta agcaaagccc tggccatagg ctcattatct ctcattaatt caacacaacc
    13141 ggcgtcagcc atgtattctc tggttcgact taaaaagaga gctagcaaaa cagtaataat
    13201 aggtaagacg tatcttaaga tgataataac cattaacaag cggttatctc ctcttctgtc
    13261 tcgcttgtaa atgacggaat aaaacaggat atcaatgaca attaataaaa tgttacttag
    13321 tacggcgacc atgagagtca atttaatatc atgatgtctg atatggctta actcatgagc
    13381 cattacagcc tgcatttcag ccctgtccag tttttccatc aagccacgag taatcgcgac
    13441 catcgccgat ttctcgctat agccactggc aaaagcattc atgtaactgg cttcgataat
    13501 gtaaacttta ggcatgtatt gcaagcccga tgcgactttc atctcttcga caacattgta
    13561 caattgcttt tcttgtaagc tacgagccgt ttctggcgtt atttgatagt actccgtccc
    13621 caataacata atgcggtcat aaagagcata agtaattaac aaagaaatgc ctgcaaatgc
    13681 gatcattaat aaagtagcaa taggcaccac tttaaaagtg attaaagcac ggataatatt
    13741 ttccaaagaa gcatgaggat acatgccttg taaaacaaac gcatcgacta tcaatcccac
    13801 caagatataa actaaaaaaa agactgcaat cacccttcgg gtctttttct catttcgcct
    13861 caactgagca cgccaatctc ctgctgtggc atgataatct gcaatcccca tggtatatag
    13921 ctcaaagttt cacagtgtaa ttttcttttg cttgaatttg ttcttctgga agtccccaat
    13981 agataaattc tttatccaaa gcagaagaaa acattgccac aaccatggat tcaaaaaatg
    14041 attttttctt ggcgttataa cgttctatcc catcattata agcttgtttg gcataagcca
    14101 acttgttttc agtgttaaca atttcctctt gtaactgcat gacattttgg ctagctttca
    14161 aatcaggata ctgttcaaaa accaaattca aattggatgc aatttttgaa attccatttt
    14221 ctgcagcaat tcgaccttgc tcatctcccg cttctttagc agcctgagcc tgattacgta
    14281 aggcaacgac atcctttaat gttgtttttt cgtaatccat gtatttttta actgcttcga
    14341 tcagtgattc aaaaacttta aatcgcctat ccaattgaat atctatttgt tttttattgt
    14401 tattaattgc ctctatcaaa ccgattagag tgttatagat gctaattgcc caaatgaatg
    14461 cgagtgcgat aataattaaa attgcgataa aaaatgtttt catcttccta tcctgtgata
    14521 aggttccttc cctaatttat agctcgaaat ttcaaaaaat aacagttaag ataagaaaaa
    14581 agaaacaact ggataacgtg ttttttgtcg caaagagaga ttcaggttgc ctgaaaaaca
    14641 acctacatag agcatcaaat ataaagacaa attgataatt aatgaactat ttgcatttct
    14701 gtaatagaaa ctcatttgat ttagataaaa tttgtttcaa aaactgacct gtaaaagatt
    14761 gagggttttc agcaaccatt tcaggtgttc ctgttgcaat aatctttcct cccttactgc
    14821 ctccttcagg accaagatca atgatccaat cagccgtttt tatcacatcc aaattatgtt
    14881 caataatgac tatagtattt ccttgttctc gcaagcgagt caacacagtt aataactgtt
    14941 tagtgtcatg aaaatgtaat cctgttgtgg gctcatcaag aatatacagt gtatttccgg
    15001 tatccctttt tgataattct cttgccaact tgattctttg agcctctcct ccagagagag
    15061 tagttgcact ttgtccaagc tttatataag acaaaccgac atccattaat gtttgacatt
    15121 ttctggcaat tacaggaatg gcagcaaaaa aagcgcacgc atcttcgaca gtcatgtcca
    15181 acacttcatg aatatttttg cctttgtagt gaatttccaa agtttcccta ttgtatcttt
    15241 tgccttgaca tacatcgcaa gaaacgtata tatcaggtaa aaaatgcatt tcaaccttga
    15301 taagaccatc accttgacaa gcttcacacc ggccaccttt tacattgaaa ctaaagcgcc
    15361 caggttgata cccccgtgct cgtgattccg gagtccctga gaataaatct ctgatatgag
    15421 taaataaacc agtatacgtt gctggattgg aacgcggagt tctgccaatt ggactctggt
    15481 cgatgtcaat aaccttgtca caaagatgca gtccagaaat gtccttaacc tcacctatag
    15541 tcattaagct ggctctgttt agtgcattgg cagcaatagg atataaagta tcattaatca
    15601 aactggattt acctgaacca gaaacacctg taatacaagt caacaaacct aaaggaatac
    15661 tgatatcaac attttgtaaa ttattgcaag acacgccttt taaatgaatc atccgatcat
    15721 ggttaaccgg aattcgatcc ttgggaattt ctatttcctt tttcccagac aaatattggc
    15781 cggtgagaga tgcgggattg ttcatgacct cttcaggaga accgctggca actacttccc
    15841 caccatgcac tccagcccca ggtcctatat caagaacaaa atcagcgctt cggatagcat
    15901 cttcatcatg ttccacaacg attacagtgt tcccctggtt gcgtaaatgc attaaagtac
    15961 tcaataatcg gtcgttgtca cgttggtgca gccctattga aggctcatcc aaaatataca
    16021 tcacccctac aagccctgaa cctatttgac tagccaggcg aatacgttga gcttctccgc
    16081 cagatagagt ctccgcgctt cgagccaaag acaaataatc cagcccgaca ttcaccagaa
    16141 accccagccg ctcaacaatt tccttattga tttttgctgc aatttcaccc ctatatcccg
    16201 ataacttcag ctctttaaaa aactgatagg cttgctcaat agaatagctg gtgatttctg
    16261 gtagattttt attatcaata aatacatgtc gagcttcttc acgtaatctt gctccctgac
    16321 aagattggca agggcgtgag gataggtatt tggcaagttc ctcccttatc ataccagaat
    16381 ctgattcacg atagcggcgt tgcatgttag gaattattcc ctcaaaacta tgccgcttca
    16441 ccatgtatcc tccatgcggc ctatgatact gaaactcgat tatttcacga ccactaccat
    16501 acaaaacgat ttgttgcatt gcttcactta aatcacaaaa aggcgtactg gtatcaaaac
    16561 catagtgcct cgcaagcgac tcaagcatgc tgaaataata cgtggttttc ctatcccatc
    16621 ccctgattgc cccttctgcc aaactggcgg ttgcatcgtg gactactctt gacggatcaa
    16681 aaaactgatc cactccaagc ccatcacatg aggaacacgc accgacagga ttgttaaatg
    16741 aaaaaagcct tggctcaagt tcacttaaac tgtaaccgca ttcagagcat gcaaacctgg
    16801 aagaaaataa aagttccttg aaatgaccat ccattgaaga aacgatggca aggccatctg
    16861 ccagattgag cgcattttca aaagattcac tcaaacgttg ggccaaatca gggcgcactt
    16921 taaaacgatc aataactact tcaatagtgt gcttggtgcg caatcctaat ttagggggag
    16981 aatccagttc ataaagctct ccatcaatac gtgcacgtac gtacccctgc gcttgcaatt
    17041 gttgcaaaaa ctggacatgc tctcccttcc gttcacgaat aacaggcgcc agaatcatca
    17101 ctttactgtc ttctggtaaa gccaatacct ggtcaaccat ttggctaatg gtctgagcat
    17161 gcaagctggt gtggtgggta ggacatcttg gctctccgac tcgggcataa agcaagcgca
    17221 aatagtcata aatttcggta atggtgccta ccgtggatcg gggattatga gaggtagcct
    17281 tttgttctat agaaatcgcc ggggatagcc cctctatcga atcaacatca ggtttttcca
    17341 tcatggacaa aaattgtcga gcataagcag acaaagattc cacgtaacgg cgctggcctt
    17401 cggcatagag agtatcaaag gctaaagacg acttcccaga accagacaat ccggttatta
    17461 cgattaattt atctcgagga agctccaggt ccaagttctt gagattatgg gttcttgcgc
    17521 cacgaatggt gatggtatgc ataaaattcg ctaatttgac ttaaaaatac atgagtatat
    17581 ctctgctata ataataaaac aaattgcaca ctatgatgcg aaatagggat tttaatatga
    17641 aatcactaat aaaaaactta cttttcattg tggtattttc aatcaactca tctgctctct
    17701 atgctaccaa tggtaatacc cgagtgattc aatgggctca acaaaccctt atggagactt
    17761 tatctgtcag ttatacttca aagccacaag attttgtcac tgttcgcaaa aattactcac
    17821 ttaatgcctg gagtggaata accggatttt tagggggtaa tatgcaaagc atacgcgaaa
    17881 aacaactaac acttcatccg gttttactgc ccgacaccca aattctctca acaggtcttt
    17941 attcgggtat gagttattgg attatcagac aatccgtagc cattgatgaa ctcaatacca
    18001 agctgacttt taccttaacc atcctgggac gatctggggc tgcaggctct ccatacatag
    18061 tgcaaagctt aagtatattg ctcaatgcat tataattaca aattaaacag gaatcacctg
    18121 aaatatggat tgtctccatg accacatgga atcatcaaca tgaaaggtat actctcgctt
    18181 taaattctgc ttcaatataa acaggactta cgaaaacccc caaggtcgag gcaaaaagtt
    18241 tttttaatga gagagtttag atacactaaa tgaccgaatt aaaaaaaatt tttaacaaag
    18301 agattggggt ttttcgtaag tcctgataaa agcaacaact ggaggacaca accatgccta
    18361 agtgtatgca ttttattttg gctactgcat taatttttgt tacaagtaca ttttatgcca
    18421 gcaatctacg ggaaatagaa caagaagtaa gtgattcagt aattactgct aaaattaccg
    18481 caaagtttgc cgaaagcagg cacttgaatc cattaaaaat ttcaattgac acagaaaagg
    18541 gaatcgtgac cctaagtggc tatgctcctg ataaacaggc tttcatagat gccttgcaga
    18601 tcgccaaaaa cacgaaaggt gtcaaggcgg ttcatgccaa agatctccac ataaagaaag
    18661 tcaataccgc tttcacagat gccttcatca cggcagaagt tgaaactgct gttctaaaag
    18721 ccaaaattct ggatgatgaa tcaataccat tggttggaat caatgccagc acaacgaatg
    18781 gcgaggtcac cttgtctggg catgtaaaaa gtgcgcgctc cattgccgcc atcattaaac
    18841 gcgtaaatga gatacctggc gtcaaaaaaa tcgttaccaa tttggaaatt caggaaaata
    18901 catgagtcca ttaacccaat ttaaattctt tttatggttt tttagccact ttaaagtagc
    18961 cttgattggt tatttaaagc ctaagttaat tcagcttacc gataatgaaa ttgttgtgcg
    19021 gctgccattg acaagacgca gccgcaatca tctgcattcc atgtattttg gtgccttggc
    19081 agttggtgcg gatatagctg gaggattaca tggtttttat catgctaaac aagcgaaatg
    19141 taaagtatcc ctggcattta aatcttttca agcccagttt ctgcgtagac ctgaatccga
    19201 tgtttatttt atatgcacag aaggggatgt agttaaaaaa atgatagaag aatcaaaaaa
    19261 atccggtgaa cgaattaata aaccaattca tatcaaagct tatatcaatt atctgaccca
    19321 tccagaagaa attgctgatt ttatccttga actctctcta aaagttataa aataacagcg
    19381 ttcgattatt atacaaatat taaccctaca aatgaaaatt gttttttcaa tctcattttc
    19441 ttaaatatca cataattatt agtcttctaa aatcacttat tattgaattg attacaccat
    19501 ttgacaaaga agccactcat gtctaatttt aaatcatgat gtcgctattt ttaccgcaag
    19561 gatttgccta tgattgacct gataatacaa actcaacctg atgacgaaac ttgcggacca
    19621 accagcttac acgccattta tttatactac ggtttggatt tgccattaaa cgaaatcatt
    19681 caaggagtgg acaagtctct ttcaggaggc accttaacac ctatgttggg aaaacatgcc
    19741 cttcttcatg gatttagaac aactgtttac atctataacc tgaatatatt tgatctcacc
    19801 tggtttcata atggtgaagt caataactcc tttttgatgg ataaacttga agctcaaatg
    19861 aaatacaaaa atgaccctta tatcaccaaa gcttctatgg cctatttaga ctacttaaaa
    19921 ctgggaggaa caattcgctc caaatcactg aacgtggact tgttaaaaaa ttattttaat
    19981 aagaaaatcc ccattttaac tggcttaagt gctacttacc tctatcgcag tgcaagagaa
    20041 tactttactc cggaaggcaa atcagtttac gatgatattc gtggtgagcc ttgtgggcat
    20101 tttgttgtac tgtgcggtta tgacgtaaaa aaacgccttg taattgttgc tgaccctcat
    20161 gcagaaaacc cactgtctca taacaattat tataaagtaa gcatcaatcg cttgattaac
    20221 tctataatgc ttggtgtact aacttatgat gcgaatctac tcataattga acctaaagag
    20281 tgataaatgc aaacagttat tgttactgat gatctggata gctggtcttt tttaagtgag
    20341 cttgccccga tagtacatgc cttggaatat cttagtgggg atgagtatca tcaaaataaa
    20401 tcatttcggg tcattaactt atgccgatct tataattacc aatcgattgg ctattatgta
    20461 tctttacttg cacaagcccg cgatcacaaa gcattaccct ctgtacacag tattcaggat
    20521 gttttaaacg tcagtttatc caaactgatc tctcaggata ctcatgaaga aatacaacac
    20581 agtctccacg atatcaaagg caatgaattt gttttaagcc tctattttgg tcaaaacctg
    20641 gcaaaacgat atgcaacatt ggcaaaacaa ctccacggtc tttttccttt accactcatt
    20701 cgcttttatc tggaaaagaa aaagcaatgg cggattaaga cagtagttcc tttgtctttg
    20761 tcggatgtac cagagcatca tctggagttt atgcgccaat cagctgaaaa ttatttatca
    20821 aaaaagagag ttcatcaatg gcgtaaaaaa caacgatttc atgatttggc cattcttgtc
    20881 gacccctccg aacctaatgc cccatccaat aaaaaagccc ttgaactttt tgtttcttgt
    20941 ggtgaggaac tgggattaaa cgttgacatc atagataaaa atgaagcaaa agcgattgct
    21001 gaatacgacg ctctttttat cagagcaact acttccgtta atcattatac ttatcgcgtg
    21061 tcacgacgcg cagcccagga gaatttggtc gttattgatg acccacaatc tataattaaa
    21121 tgctccaaca aagtttatct tgcagagctc ttaagcagtc atcaaatcat gacgccagac
    21181 accttattca tcagcaaata tgataaggaa ttacccaaga ttgaattccc ctgtgtttta
    21241 aaaaaacctg acagcgcctt ttctcatggc gtggttaaaa tagatgatga aaaatcttta
    21301 caaaaatctc ttaaccagtt ttttaaaaat tctgatttgg tagtggttca accttttata
    21361 ccgactgaat ttgattggcg catcggcatc cttgataata aagctctttt tgcctgccgc
    21421 tattttatgg caaaaggcca ctggcaaatc tatgattggc attgccagga agaaaataaa
    21481 gagggggata gtgaaacact accccttcat gaagtgccag agcagattat taaaatagct
    21541 ctgaaaagta cacgtttgat tggtgatgga ttatatggtg tcgatataaa aagtcatggc
    21601 aataaatatt atgttataga agtgaacgat aatcccaata ttgatcatgg ccttgaagat
    21661 aaaatactag gccaaaatct atatgagcag atcatgagtg tcttcttaca acgaattcgc
    21721 aggaaacatg gttatgtctg attttcctat tttttctgtc ttggggattg aaatagaata
    21781 tatgctggtg gataaagaga gtttaaatgt ccaacctaaa agcgatctta ttctaaggac
    21841 tttggcaggc catcaggtta atgaaataga attaggagac attgcgataa gcaatgaatt
    21901 agtattgcat gtccttgagt taaaaaataa tggtcccaaa ccactacatg ttccagtagc
    21961 tgaacatttc cagaaaacca ttagagagct ccagcccctt cttgctcaac acaatttatt
    22021 gctattacct actggcgctc atccctggat ggacccaaat actgaaacaa ttcgctggcc
    22081 tcatggtaac catgatatct ataatcaatt taattcaatt ttcaattgcc agggacatgg
    22141 ctgggcaaac ttgcaaagta tgcatgtcaa cctgccttat tccaatgaag aagaattttg
    22201 tcaattgcat aacgccatac gcttgatttt acctttatta cccgccattg cggcaagtac
    22261 tccattcctt gatggccaag ctacaggatt actggattcc cgcttgtact tttatagccg
    22321 caatcagcaa cgcattccct ccatcagcgg cgatattatt cctgagttta ttagaacaga
    22381 aagagaatat caggaacaaa ttctcgaacc catgtatcga gacattagcc catatgatcc
    22441 caaagggata ttacaatatc aatggctaaa ttctcgtgct gcaattccca agtttgacat
    22501 gaaggctatt gaaattcgca ttcttgacag ccaggaatgt gtagaagcag acattgccat
    22561 agcaaaagca atcaatgcca ttttaaaata ctggcaaagc acctcgaatt tttatcttga
    22621 taatccatgc gacaccaaga gtttaaaata cgtttttgat gaaaccataa aaaacggatt
    22681 aagttgtcat atcgataaca aaaaattact ggagcaatgg caattacccc ttcaagggat
    22741 gtcagtcaat accatctggt cactattgat cgaaagaatt agtcatgaac tggataggaa
    22801 gcagcaactg gctctggaac ttattttaaa acagggaaat ttgagtgaaa gaatcctcaa
    22861 ggcttgtagc tccagtatca atcatgattc tttaaaacaa gtgtatacgc aattagcaca
    22921 ttgtttgctt cgtaatgaac aatttacccc ctgatataat gaaaaatata gcccttttaa
    22981 tcagttgtga acatgctgtc aatacagttc cctatgaata tcaaaccctg tttgaacctc
    23041 ataaagacgt attagaatct catagaggaa ttgattttgg tgcgctagaa attgcacaat
    23101 cgattcataa aaacaaccat tgcagtttgt ttcaagccac ctgtacaagg ctcttggttg
    23161 attgcaatag gagtatcaat catccccatt gtttttccga gattacaaaa aatttatcat
    23221 cagacaaaaa gaaaaaaata ctggatgaat tctatttgcc gtttcgcaat cgcgtcatgg
    23281 accacattca ccaactgatt agccaaggac tgcaagtttg gcatctgtcc acacacagtt
    23341 ttactcctgt aatgaacaac atcattcgca cgactgacat tggccttcta tatgaccctc
    23401 ggagtaattc agaaaaaatc ttggccagac aatggcaaaa agaaatcaaa atactccatc
    23461 cccagttaaa agtacgtatg aattatcctt ataaaggaat cagtgatgga tttactacat
    23521 ccttgcgtaa aaaatacagt catcatgaat acataggcat tgaactggaa gtcaatcaaa
    23581 aacttgtctt tcacaatcac agcctggaca cgctcaaaaa tacactgtca ttgagcgtat
    23641 caaacacgat taaggcttat caaaaatcag agaggtaagc gacaacattt ttttcaagtt
    23701 gcaatttaaa aaaaccaagt ggctgaatta aaaatttttt ttacaaaaaa gattgagttt
    23761 ttacaagcac tgatcatgtg ttaatatttg cttaagtatt tttctttata atatcacaag
    23821 ttaatttttt taatattgaa atgatttcag aaaagatcaa gcttttagaa tcctttaagc
    23881 aaaaccaatt ctctaaatta attgaaaaaa ttaattcaaa aatccttgcg actttaaaaa
    23941 gcggacattt caaccccaga ctagccccct acttggaaaa aatgaataaa gacggggttc
    24001 cattcaccga ttttgatctt gacccggaaa atatttctca aatcaagaaa ctcattaacg
    24061 ccctttacca tgcccgttta gcttttctgg atttagagaa tattgacata gggaacatca
    24121 aacgaactat ccctgatctc aaattacttt acagcaacac gattcaccag gcctatcaag
    24181 ccggttatct aatcactaac ctggatgttg atttgcagga aatgttcaag gaagagattg
    24241 atcttattct tcctgtcatt ggaaaattac aagcttttgc tggtgaacac caggaatgca
    24301 ccaagcattt ggcagaatct ttgaaggatt ttcctataag ctataaagca ggagaagtta
    24361 caggcattgc cttagagcaa atgcaaccga gaacaggaga ttgggattac caattcctca
    24421 ctcaattcag tgccgtatta cctggttata tagaaaaatt aactcaatac attcaacaat
    24481 actcttcgca aatcaaagaa aaagagccta ctctaaataa tgaaaagctg gaagagttac
    24541 aaaacgcatc attaaaatta ctaaatgacc tggaacatct gaaagggaat gactttttta
    24601 tatcccttaa agttttaaaa tacattcaca ttatccgtaa tatcatcaca ttggcgacaa
    24661 gctctgttga acaaatggga catttcagcg attcatctca ggatgtcatt cgtgataatt
    24721 tggctcaatt aaaatacgtt gtattaccta ctttatttgg gttagtggat aaaattgaag
    24781 acaatgccat gctgaagcct ggtacactgt ctattccgtt aatggaaaaa atcaaacctc
    24841 tataccagct tttaatttat tacgcatcca aacccgtcaa tttcaaagaa aaaggagaag
    24901 agttactcag tatagaggac tcccgttttc tcgctttaag actggaaaga acatatcaac
    24961 gaattgatga agctaacaaa gcccttttta aaattcaaaa agcgcaagaa gctctggata
    25021 atttttacaa aatattggac acccccccct ataataagca acccatacat caattaccga
    25081 aagaaattaa aaaccaactg attacatatt acaaaataat cagtccttat atgatggatg
    25141 tgaatcctga tctcaatatt ttgttgatcg atagttttca gggcccggaa tcctggtctt
    25201 cttatttaaa gaagccgtgg cgttggttaa gaggcactct tccagccgat cacgccagtt
    25261 ttgttttagc gcaaaaggaa gcattacaaa ctttaattag caaaaagaaa gccactcaac
    25321 aatttcatat cgaattgaat agagacctta tcgaatctgt tcacaaacaa acaaatctgg
    25381 ttttattccc ttatagtgaa aaaacgaatg tctttcttct agatgagaca aaagcactaa
    25441 accctgctaa tggcgctacg gacaaactaa aattcaggcc agagaaggga cataatatac
    25501 tggttaatcc tgaagattta acatctgaac aggctcttga cctctatcaa tggtatagaa
    25561 acaaacgtga gaaattcaga gttgccagca aagcatataa tgaatttatt accttattaa
    25621 aaaagcaaac caagacaatc gctgaaacgg aaggc
//
LOCUS       DATNSN010000029        25284 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.029, whole genome shotgun sequence.
ACCESSION   DATNSN010000029 DATNSN010000000
VERSION     DATNSN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.029"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(170..3436)
                     /locus_tag="VAH76_001022"
     CDS             complement(170..3436)
                     /locus_tag="VAH76_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO1385669.1"
                     /translation="MLNEAISKMPEAFEAKILLRGEEYFQKGQVLNIRLSDGLLKGRV
                     KGSASQIYDIHMDLKSWPAKPSRCSCAYQYNCKHAAACLFALRDREKINLQSLPSNKL
                     DRKLDVWLKNLRAQEASEIKREEATHHLIYLIDLKLRGYEHKVSIKLALAKLLKRGGY
                     GKKIIFNTLANSKKQHFMGDDNDIVAQLLFKCNVSGWFDNLLIRNTELLDKIIATGRA
                     FFIENEDIPIKKGETLTGECQWILSWNGNQILKLRHDNQNIEPLLLDESWYFDIKNAV
                     IGHLNSPYPIKQLRHLLEAPPIPLDQAELLAKKMSKNCPEFPMPNVFNQKETKQLKPI
                     PVLILDAVTEQEEHRWIDDGEENNSLYIAKIFFDYEGLLVPFAEECEKVVSLKGDVLV
                     EYQRDQEFEDLKIQELRNSIDLRPFHQWEKYHWNLPDHSFFILKDVQIVSDLESLYQQ
                     SLPYLKDHGWRIEFASPFYQEIVSADEVEWYSDIQESTTDFFSYQLGILVEGKPVSIV
                     PLVADLIQRYKGNDLDNLPDEQLVKLPLLDGRLLQLPMGRIKPLVRLLLQFGLRQIDQ
                     NQQLQINKYQLVLMQEVELAIAATKSRWQGAESLREELRKLVRLNDLPEIKVPSGLQA
                     HLRDYQHYGLNWLQFLRVSRFNGVLADDMGLGKTVQTLAHLQYEKEQSRLHKASLIIA
                     PTSLVGNWFAEAKRFTPEIKVLIYHGSDRHQGNFDDYDLIISTYGLIHRDKEKFVGYP
                     FYYLILDEAQFIKNARTKTTQIIQQLKASHRLCLTGTPLENHLGELWSLFHFLMPGLL
                     GDAKQFRLWFRTPIEKYADMGRRDLLAKRVQPFLLRRTKNQVANELPPKTEITRTIEL
                     VGAQRDLYEAIRMSMEKKVRDAIARQGLGKSHILLLDALLKLRQVCCDPRLLSMSEAE
                     IAHGTSCKLDALMELLDNLVEEGRRVLVFSQFTSMLKLIEELLIARQYDYLKLTGQTQ
                     NRQALVEQFQQGDTPIFLISLKAGGTGLNLTRADTVIHYDPWWNPSVEDQATDRTHRI
                     GQESPVFVYKLITSGTVEEAILGMQEKKRQLVEGILSTDASRSMVLSEEDLNQFFMPL
                     "
     gene            3720..5330
                     /locus_tag="VAH76_001023"
     CDS             3720..5330
                     /locus_tag="VAH76_001023"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEO1385670.1"
                     /translation="MNAPLTVLMAQINPTVGAIDANTNKILDVIKNHQASHDVIIFPE
                     LTLSGYPAEDLLFRKEYHDKILINLHDIQDTTKDCYVIVGHPMMHIGDCYNGFSIFYQ
                     GEKIREYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCVCEDLWQKGPVDDL
                     LDSGISILISLNASPFDYRKYQLREELLRSYAKQGVSIIYVNQIGGQDDLLFDGQSLA
                     MDNQGIIRALAPAFEESLCTVNIDGNKIDGSITPYLDKEALIYKALVCGTRDYVRKNN
                     FPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASISNEDALEQLSNLKVA
                     HSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSN
                     KSETAVGYATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELK
                     PNQTDQDSLPEYGELDAIIMAYMEQNLSPSEIIAKGFKPQVVNKIIQLIKRNEYKRRQ
                     AAPGIKISNLAFGKDWRYPITNGFNQCI"
     gene            complement(5382..6830)
                     /gene="ravH"
                     /locus_tag="VAH76_001024"
     CDS             complement(5382..6830)
                     /gene="ravH"
                     /locus_tag="VAH76_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavH"
                     /protein_id="HEO1385671.1"
                     /translation="MDKEQGAYMPPKDGKELLDIIDNLNEFAEKLLSRIADESDEGCK
                     QLVHVLESARARYNKPIESWLKGWFYQFTRKRGQEIDMAIKGMEAFPDAYTRLQEFKL
                     LVGKGEWEHGSFNYYLFDELIKSVPGYKPLESELVHPIILKLRQKINKRIDEFMSQYL
                     ATQKLIEARKQELLQTHQSAQKFIDNVSITNDLEAAKKSAKENKGVIQFCLIFESDIW
                     KLSWVAATGKVYALEPGEELIQILIDGKIQDIVKDIDNLSALHLKQLKQECVKAREML
                     LGRVQLLINPKDPRTQAELTNDVLKENGTSATFVLRGKPNEYSLWWINTLGFAHEISL
                     ESYASMKTWLNDQSHPLKEEHIPQLKTYLLNVNTVKSIDVKEDMKEFKAQLQECLAAG
                     PKSKTPPASGATKTTNAKKLDLRLFGDVERCLRSQLSERQEEKAAIQEPGKINLNKYE
                     NIATLFGNRAKNMEQHEGQQSKPNDSLSIRSI"
     gene            6996..7634
                     /locus_tag="VAH76_001025"
     CDS             6996..7634
                     /locus_tag="VAH76_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEO1385672.1"
                     /translation="MRITLLTTAILATGVASAAVPVDGWYASAFGGYTYIPPNIYNFA
                     YGVFRDEVGYNNGYNAGGRIGYQSNPIRYEVEYTYLRGEARKFNLNFITQLGVTGFTS
                     ANTIMANIYYDCPDMLPAIAPFIGLGIGYASLRTELDSIGPFRPSYFTTSDSAFAYQG
                     TVGITYNFSENYAANLAYRYIATDNSSNFGKTYQAHLASVGAIYRFDQGNYK"
     gene            7644..8408
                     /locus_tag="VAH76_001026"
     CDS             7644..8408
                     /locus_tag="VAH76_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385673.1"
                     /translation="MKKLTQLSVLSGLLLSGSTFAFNQPQGFYVGILGEISHGPSSED
                     IAFLYEDRNTYTGTVKYSSVSGGGGAVLGYKISHYRIEGEVLYNRISTGPLTINTCTL
                     QSPNVFTPTGVCPPGVPPSGYDNFRVNGTGYDGSSAALYGLLNLYYDFFSSVHESTYV
                     PYIGLGAGQVRIQNRSNFVKTVLGRLPPSFGTSSTMVSGAGQGIIGVGYLMDDFAWLA
                     MDFRFLTTNSLSDFNNKRYTLSTFNVGVNFTFDKST"
     gene            complement(8400..9452)
                     /locus_tag="VAH76_001027"
     CDS             complement(8400..9452)
                     /locus_tag="VAH76_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO1385674.1"
                     /translation="MNENHKELISIGLTVSIVIFSLYIVHKFIPSMIWAAIIVIATYP
                     LYTRWRKLFGNKHNTSAFLFTTLMGLLFLIPLSWLIGILIKESQLFINFLQHINKEGG
                     AAPEFFKNIPLVGDDLIQYWDVNIGKPGSIKEFLSNLHVTLTPTSYYIKQIGVNLAHR
                     SFQVGFTLLTLFFFYRDGDKLLLQIQHIGEYCLGDRWFRYSDRLPSALRGTVNGTIVV
                     GIGVGILMGICYALVGFPAPTLTGFITALAAMIPFVVPIVFIIVALILLSVGSLIGGI
                     IVLVWGTLVMFVADHFVKPVLIGGAIQLPFLAVLFGILGGVETLGLLGLFLGPMVMVL
                     FVTLWQEPQGHKKSSG"
     gene            complement(9452..9934)
                     /locus_tag="VAH76_001028"
     CDS             complement(9452..9934)
                     /locus_tag="VAH76_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEO1385675.1"
                     /translation="MNQVNLANRVWQDPVYFIAFGFGSGLMSIAPGTWGTLAAIPLYL
                     LMINAHWSIYLLWTALAFALGVWVSDRVTEDLGIHDYKGIVWDEVVGYLLTMFLAPKG
                     ISWMLIGFILFRIFDIWKPQPIRMIDQRVHGGLGIMLDDVLAAVPAWCIMQILVWSFA
                     "
     gene            complement(10024..10980)
                     /gene="thiL"
                     /locus_tag="VAH76_001029"
     CDS             complement(10024..10980)
                     /gene="thiL"
                     /locus_tag="VAH76_001029"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEO1385676.1"
                     /translation="MNEFTLIDHYFKSIPCKRNDVLFGIGDDAACLQIPDGMHLLVST
                     DTLVSGIHFLPQWDPYDIACKAVMVNISDMAAMGAQPCWATLALTLPELNQSWLDSFA
                     RGIKDSLGLYNVDLIGGDTTKGPLSITLTIMGLTPQGQAIRRCGAQAGDVIMVSGELG
                     AAGLAVKLLDGHKKVNAKDKIELMNKLLHPKPRVDLCHMLRKYATSAIDISDGLGADL
                     NHICVASGVGANLFEEAIPRHPLLNQYCPEQALDLALSGGDDYELCFTVSTQQLDSFN
                     RDLKKENLICFPIGVIEEKAGLRIQSADSPSKELQPRGYSHF"
     gene            complement(10973..11416)
                     /gene="nusB"
                     /locus_tag="VAH76_001030"
     CDS             complement(10973..11416)
                     /gene="nusB"
                     /locus_tag="VAH76_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO1385677.1"
                     /translation="MEKQSIRGKRRARKFALQALYQWLMSGTDLHEIEAQFRTINNMD
                     KVDGEYFCRLLYGIPTHVEALEASLLPYLDREINALNPIELTVLRIGSFELFHCPEIP
                     YKVILDESVSLTKEFGSQEGYRYVNGVLNNLAKQVRSVEVSLDNE"
     gene            complement(11474..11941)
                     /gene="nrdR"
                     /locus_tag="VAH76_001031"
     CDS             complement(11474..11941)
                     /gene="nrdR"
                     /locus_tag="VAH76_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEO1385678.1"
                     /translation="MYCPFCHAEETKVVDSRLVADGAQVRRRRECLECHERFTTFETA
                     ELIMPLIIKRDGRREPFHIDNLRSGMLRALEKRPVSVDDLEKAIISITEEIRRRGERE
                     IDSQVVGELVMKELFRLDHVAYVRFASVYKRFKDVSDFRQTIDQMKNEDKEKS"
     gene            complement(11944..13197)
                     /locus_tag="VAH76_001032"
     CDS             complement(11944..13197)
                     /locus_tag="VAH76_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO1385679.1"
                     /translation="MFDESYTIKNFDDVLFKAISDEKRRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEELAISRAKLLFGAHYVNVQPHSGSQAN
                     AAVMMALLSPGDTFMGMALPHGGHLTHGSKVNFSGKLYHSVEYGVDSNTGLIDYDALE
                     KLALQHKPKLIIAGFSAYSRILDWARFREIADKVGAYLMADIAHVAGLVAVGLYPSPV
                     PYADVVTTTTHKTLRGPRGGLILCKENEEIEKKLNSSVFPGMQGGPLMHVIAAKAVAF
                     AEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEAD
                     AALGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREITLLSNWVADVLDN
                     VHDETNISRVKTQVLLLCREFPVYA"
     gene            complement(13225..13536)
                     /locus_tag="VAH76_001033"
     CDS             complement(13225..13536)
                     /locus_tag="VAH76_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEO1385680.1"
                     /translation="MRDSLKMLLVGSISMIIIACAASPLSESTGEYLDSSTTTAKVKA
                     SLVDQLGTTGFAVKVKTYKDQVQLSGFVDSQKIKQRAGIIAAGVDGVKSVRNDLIIKT
                     R"
     gene            complement(13661..14098)
                     /locus_tag="VAH76_001034"
     CDS             complement(13661..14098)
                     /locus_tag="VAH76_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385681.1"
                     /translation="MKYLIPCVVLFPLLSSCTVIQEDFYQPEYLGPAPRVEVTPSYPQ
                     GHYHRHYNQRYHSNNRYYSAPRGKVHYEHAKTQGHAAVVTPPPSQARVEVQKNVHGHN
                     ANNGQVHGHAHDNGNVHSHPDSSNVHGHDTSNPEEQKKIHGHS"
     gene            complement(14164..14568)
                     /locus_tag="VAH76_001035"
     CDS             complement(14164..14568)
                     /locus_tag="VAH76_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385682.1"
                     /translation="MKLKSLLFVVCFSLFSNVFAANLHLHPKADNADKKSIQKSVTYP
                     GYCQIEIINQSFTDVRVFGTFDDGTTIDFNIYRYESPHYISLFYNFYCHSGMYITITS
                     PYYTIYSGWTNVNSTIRVVPYLKEAKAELSTR"
     gene            14809..16071
                     /locus_tag="VAH76_001036"
     CDS             14809..16071
                     /locus_tag="VAH76_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385683.1"
                     /translation="MNKRLTIMGIILLVVFGGIIAFNLIKAYMIKQFFASYAPPAVTV
                     SSAVAQAVDWKPTIDAVGNFVAINGVDVNSEASGNVVQIHFESGQYVEKDEPLITIDD
                     SIDQALLKFNQSELTLKELSYKRQTDLFKRGATPSSSVDEAKANLQQAQAKVEQIQAQ
                     IKQKHIGAPFSGRLGIRQVNLGQYVTPGQTSIVSLQSLDPLYLEFYLPEQYYKRIHLN
                     QPVTFRVEEFPNILFEGVIHAVNSKVDLTTHNVQVQALLPNCPADAIQDPTKSPLIKV
                     RKQVRGDKLIVSCSTELNKQNKIRNFVFIPGMFASIEVDQPAEPNTIIVPSTAISYSL
                     YGNAVYIIEKSKEGKKNKDGTDQLVVNRVFVTTGEQQGNYTVIKKGIKVGQLVVSTGD
                     LKLQNGTPVVINNSVKLDTDSDPNQLGQ"
     gene            16188..19235
                     /locus_tag="VAH76_001037"
     CDS             16188..19235
                     /locus_tag="VAH76_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEO1385684.1"
                     /translation="MKFTDLFIKRPVLSIVVSMLIFLFGINSIYKMQIRQYPKMENTV
                     ITIMTGYPGADAELIAGFITTPLESAVASAEGIDYMTSSSQQGLSTITLNIKLNFDPQ
                     VAFTDVMSKVQQTLNQLPKESQQPVILKKSDSSTALMYISLDSKDMTPQQITDYATRV
                     VQPQLQTVDGVAKVDILGGQTYSMRIFMDPIKMAALNVTPADVSSVLASNNFLTAAGS
                     TKSEYVAINVTAKTDLNNVGEFERLIVKSDKGSIIRLRDIAKVELGSQNYDSSVTFNS
                     KKAVFISITPTPTANPLTVINDARKLFPSIVREFPPSLTGTIVYDATAFIRASIHEVM
                     ETIIEAAVIVIIVIFLFLGSVRSVLIPVVTIPLSLVGVCTLMLILGYSINLLTLLAFV
                     LAIGLVVDDAIVVVENVHRHLEEGKTPFESAIIGAREIATPVIAMTITLAAVYAPIGF
                     MSGLTGALFKEFAFTLASAVIISGIIALTLSPMMCSKILSEEATSGKFAIFLDEFFNK
                     LKRVYQNALHSILDTREIMLLLAGVVILMLPYLYSNTAAETAPDEDQGFFFVLATAPQ
                     YATLNYIEAFTKPFDAIYKSFPETENYFTVNTSQPISGMVLKPWDQRKKSQFALKQPL
                     QNKLSKIAGLNALAIVPPPLPGGGGGPAVQFVVKTTNDFQSLMDVSNQLTEKARNSGL
                     FIYIDNSLKFNQPQVEFSINRAKASEMGLDMRALGSTLTSALSNNYINYFNLEGRSYQ
                     VIPQLARKFRLTIEQIGQIYVKTLNGTMVPLSTVITPTEKTQPNAATHFQQLNSATIQ
                     AVMMPGKTLGQGLEFLQKAAQEVLPKGFTYDYGGQSRQFMQEGSALIFAFLFAIIVIF
                     LVLAAQYESFRDPLIVLISVPMSICGALIPLNLGAATINIYTQVGLITLIGLISKHGI
                     LIVDFANHLQREKNLDKRAAVEEAAGIRLRPILMTTAAMVFGVIPLLIASGAGAASRF
                     DIGLVISTGLLIGTCFTLFVVPTLYTYLAEDHRHKPNEPAEIQ"
     gene            19491..22256
                     /locus_tag="VAH76_001038"
     CDS             19491..22256
                     /locus_tag="VAH76_001038"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO1385685.1"
                     /translation="MDKTYSPEAIEKALYKKWESHHYFQPRGEGKRFCIMLPPPNVTG
                     SLHMGHGFQHTIMDALTRYHRMLGDKTLWQPGTDHAGISTQLVVERQLEAQGISRKDL
                     TREQFLDKVWQWKEESGNTITQQMRRLGASVDWSRERFTMDEGLSAAVQKVFVQLYEE
                     GLIYRGTRLVNWDPKLGTAVSDLEVLSEEEDGFLWHIRYPVVDSEEFLIVATTRPETL
                     LGDCAVAVHPDDSRFRHLIGKQVHLPLCDRTIPVIADDYVDKEFGSGCVKITPAHDFN
                     DHEVGKRHQLPQINILTKKGTINKNAPLKYQGMDRFVAREQIIKDLEQEGLLAKTEPH
                     KLKVPRGEKSNVIIEPLLTDQWYVKTKPLAEPAIAAVKKGDIRFIPETWDKTYFQWMD
                     NIEDWCISRQLWWGHRIPAWYDNHGNIYVGYSENDVRFKHKIDQSTPLKQDEDVLDTW
                     FSSALWPFSTLGWPERTPELEQFYPTSVLVTGFDIIFFWVARMIMMGLKFTGKIPFKE
                     VFITGLIRDSEGHKMSKSKGNVLDPLDIVDGIDLDSLIAKRTSNLMLNSVRDRITKAT
                     RKEFPEGISAYGTDALRFTYCSLASTGRNVRFDLGRVEGYRNFCNKLWNAARYVLLNT
                     DEEQIDFGDGAFQYSPADQWILSRLQNTVSKVHHYFETYRFDLLANTLYEFVWHEYCD
                     WYLELSKPILQDEQALSAMKRGTRRTLIHVLDQILKLLHPLMPFITEEIWQKTTKFTS
                     ENGISIMLSTYPKVNEEFINPAIEEELDWLKSAIQSLRTIRSEMSISPAKLIPLYIRN
                     ITPELKERIAKYEKILKTLSKIDKINYLAPDEKVPVSATAVLGEIELLIPMADLIDKE
                     AELSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQLAKDKLLQH
                     KNRIESL"
     gene            22378..23625
                     /locus_tag="VAH76_001039"
     CDS             22378..23625
                     /locus_tag="VAH76_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEO1385686.1"
                     /translation="MVTQFNTLKSYIFPIILLLSILLGGLTGYVFNQNVAWLKPFGDI
                     FLNLIFTTIVPLIFFSVSSAIARTGSIGKLGKIFYSMTVIFLFTGIVAAVYALFVVLL
                     FPPAQNMPVLLESSKQLSPTNLPDRLIEIFTVPEFSKLFSHEHILALVLFSILVGLAV
                     AHSKDKGTIFNSFLKAGEDVFMSVFRLIMYYAPIGFFAYFAVIVSEIGPQLMENYLRI
                     AILYYTASVFYFFFALTAYAYLAGKSQGVRLFWGNIFLPAATSIATCSSAASIPANLM
                     ATKSMRIPAEIYETSIPLGSIIHKDGSVMGGVFKIAFLFGIFHLPFTSLPVLMMALGV
                     SLLVGTVMGAIPSGGMLGELLILSVYGFPPSVLIAVAAISIIIDPIATMVNVTSNSVC
                     SMMIARLVEGKNWFLRSSDENNL"
     gene            23714..24175
                     /locus_tag="VAH76_001040"
     CDS             23714..24175
                     /locus_tag="VAH76_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385687.1"
                     /translation="MKKILYVIPGILLITNSFALDIPGNITNSNKDLLPSPFPVYVIE
                     GSAVVNHPYPGAKKVLLPTDNGYVDYPGCYIACYSHNTGVYAISPTISVMGQIRVKGQ
                     YDARICQPDGYKNQDISAAYQFKQLCAEKIPACQNNSCWAGGDTGGWFGIQ"
     gene            complement(24276..25271)
                     /locus_tag="VAH76_001041"
     CDS             complement(24276..25271)
                     /locus_tag="VAH76_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385688.1"
                     /translation="MSKNESGTAKPIIKSTLMFFNSKLTDLNDKRLQLNRGKGLAKYL
                     AKSQKNTRDLAPLDAALGVMSFSLYMLRFSANVGLLAHLILVESQEKTNHKIRRDIYY
                     SLLNDSLWCVVNLTQFFWLSYRNSISAGLQGMQLETLAQLIDILVMIIRYQQDKKEYD
                     LKYNQAMGMERSRLAIEWQNKELNGLRSLLTSLSVVIAFGLFSFSGVAVPLSPVISSI
                     ILISSLLRVLIDIEKDRQLIHQLKLNETSREQIKNEQLAMTNARMKDLNKIILNSVFL
                     PIGFFLLITTPIPLTIIACVSMLLVHWLITNLINMRYSPNISVSPTIQTAELSLC"
BASE COUNT         7733 a         5300 c         4664 g         7587 t
ORIGIN      
        1 taaagcataa gccatgatag gtaactttgc tactgaaatc tttaaaataa atatagggtt
       61 tattatccac tccctgacgg tcgcggctcg gtttgatggt tgaggattgg tttgatccat
      121 aatccgagcc gcgaccgtca gggagcggat aataaaccgt tacttttatt cataaaggca
      181 taaaaaattg attcaaatct tcttcactta aaaccattga cctggaagcg tctgtagaca
      241 aaatcccttc cactaattgc cttttcttct cttgcatacc aagaattgcc tcttctacag
      301 tacctgatgt aatcaacttg taaacaaaaa cagggctttc ctggcctatt cgatgtgttc
      361 tgtcagtagc ctggtcctca acagaaggat tccaccaggg atcataatga atcacagtat
      421 ctgcacgagt cagattaagt cctgtgcctc ctgctttcag actaattaaa aatattggcg
      481 tatcaccttg ttgaaattgc tcgactaagg cttgtctgtt ttgggtctgt cccgtcaatt
      541 tcaaataatc gtattgcctg gctattaata attcctcaat cagttttagc atcgatgtaa
      601 attgtgaaaa taccaatact ctccttccct cttcaaccaa attatcaagc aactccatca
      661 gtgcatcgag cttgcatgaa gtaccatgcg cgatctcggc ttcagacata gacaacaatc
      721 ttggatcaca acaaacctgc cgcaatttca acaaggcatc aagtaataat atatgacttt
      781 tccccaatcc ttgccttgct atggcatctc tcaccttttt ttccatgctc atgcggatag
      841 cctcataaag atcccgctga gcaccaacca actctatagt acgagttatt tccgttttag
      901 gcgggagttc attggctacc tgatttttgg ttcggcgcag taaaaaaggt tgcactcttt
      961 ttgctaacaa atcccttctt cccatatcgg catatttttc tataggcgtt ctgaaccaaa
     1021 gcctgaattg ctttgcatca cccaatagcc cgggcattag aaaatgaaat aaagaccaga
     1081 gttcgcctaa atgattctct agtggagttc cagtaagaca taaacggtgt gaagccttta
     1141 attgttgaat gatttgtgtt gtttttgtcc gtgcattctt aatgaattgc gcttcgtcca
     1201 aaatgagata ataaaagggg tatccaacaa atttttcttt gtccctgtga attaatccgt
     1261 aggtagaaat aattaaatca taatcatcaa aattgccctg atgtctatca cttccatggt
     1321 aaatcaatac tttgatctct ggcgtaaatc gcttggcttc ggcaaaccaa ttaccaacaa
     1381 gactggtggg agctatgatt aaactggctt tatgtaatct tgattgttct ttctcatatt
     1441 gtaaatgggc gagagtctgc actgttttgc caagtcccat atcatccgct aatacgccat
     1501 taaaacgact gacccgcaaa aattgtaacc aatttaaccc atagtgttga taatccctca
     1561 aatgagcttg aagaccagat ggaaccttta tttctggcag atcatttaat ctgaccagct
     1621 tccttaattc ttctcttaat gattcagcac cttgccaacg tgatttggtc gcagcaatag
     1681 ccaattcaac ttcctgcatt aataccagtt gatatttatt aatctgtaat tgctgatttt
     1741 gatcaatttg gcgcaagcca aactgtaata acaaacggac caatggctta attcgcccca
     1801 tgggcaattg caataaacga ccatccaaca atgggagttt aaccagctgc tcatcaggta
     1861 aattatctaa atcattccct ttgtaacgtt gaatcaaatc cgcaaccagc ggcacaatac
     1921 tcactggctt gccttcaacc aaaataccca attgatagga aaaaaaatcg gtcgtacttt
     1981 cctgaatatc ggaataccat tccacttcat cagcagaaac aatctcttga taaaacgggc
     2041 ttgcaaactc tatacgccat ccatggtctt ttaaataagg taacgattgc tgataaagac
     2101 tttccaagtc tgaaacaatt tgcacatctt ttaaaataaa aaaggagtga tcgggtaaat
     2161 tccaatggta tttttcccat tgatgaaatg gcctcaaatc aattgaattt ctcaactctt
     2221 gaatttttaa atcttcaaat tcctgatcac gttggtattc aactaataca tctcctttta
     2281 aagacaccac tttttcacac tcttccgcaa aaggaacaag caatccttca taatcaaaaa
     2341 agatttttgc tatataaaga gaattatttt cctctccgtc atcaatccac ctgtgttctt
     2401 cctgttcggt aaccgcatcg agaattaaca caggaattgg ttttaattgt ttcgtttcct
     2461 tttgattaaa cacattgggc atgggaaatt caggacaatt tttagacatt tttttggcca
     2521 ataactctgc ttgatcaaga gggataggtg gtgcttccaa caaatgacga agctgcttga
     2581 tgggataagg agagtttaag tgacctatta ccgcattttt aatatcaaaa taccaacttt
     2641 catccaataa taatggctca atattctgat tatcatgcct taattttaaa atctgattgc
     2701 cattccagga taaaatccac tggcattcac cagtcaaagt ctctcccttt tttataggga
     2761 tatcctcatt ttcaataaaa aaagctcttc cagttgcaat gatcttatca agaagctctg
     2821 tattccgaat caaaagatta tcaaaccaac cggatacatt acatttaaat aacaattgag
     2881 ccacgatgtc attatcatca cccataaaat gttgtttttt tgaattagcc agagtattaa
     2941 agattatttt ttttccgtaa ccgccgcgtt ttaatagttt ggccaaagct aatttaatgc
     3001 tcactttgtg ctcataaccc cgaagcttta aatcaatcaa ataaatcaga tggtgtgtcg
     3061 cctcctccct ttttatttct gatgcttctt gtgctcgtaa attttttaac caaacatcta
     3121 attttcgatc tagtttgtta gaaggcagtg actgcaggtt tattttttct ctgtctctta
     3181 atgcaaaaag acaagctgcg gcgtgtttgc aattatattg ataagcacat gaacatcgag
     3241 aaggttttgc tggccatgat tttaaatcca tatggatatc ataaatttga cttgcactac
     3301 ccttcactct ccctttgagc aagccgtcac ttaagcgaat attcaatacc tggccttttt
     3361 gaaagtactc ttcaccccga agcaatattt tagcttcaaa agcctctggc atttttgata
     3421 ttgcttcgtt taacatacct aggatttgtc caggaaaaat cgtatattgt agtcattttt
     3481 tacgcttttt gacaatagaa tgggttgttc tatccgctcc cttacggttg cggctcggtt
     3541 ttaaccatga tcctatctga ctcagagtga accataatcc gagccgcgac cataagggag
     3601 cggataaatg caaaaataaa aacttctcca attaacctga ctaccatcct cttcatctgt
     3661 agccatatca atgccctcta agctacaatt cctatatacg aattaattga cagattttca
     3721 tgaatgcacc cttgacagta ctgatggctc aaattaatcc aacagttgga gctattgacg
     3781 cgaatacaaa taaaattctt gatgtcatca aaaaccatca ggcaagccat gatgtgatta
     3841 tttttccaga attaactcta tccggttatc cggcggaaga tttactcttt cgtaaagaat
     3901 atcatgataa aatactgatc aacctccatg atattcagga tactaccaag gattgttatg
     3961 tcattgtcgg acatccaatg atgcacattg gtgattgcta caatggcttc agcatctttt
     4021 atcaaggaga aaaaatcaga gaatatcaca agcaaaaatt gccaaattat ggagtttttg
     4081 atgaagcaag atattttact cccggtaaaa aagatccttg tgttttatca attaaaaatc
     4141 ataaattagg gttttgcgta tgtgaagatt tatggcaaaa aggtcctgta gacgatttac
     4201 ttgacagtgg catttccatt ttaatcagtt taaacgcctc cccctttgat tatcgtaaat
     4261 accagctgag agaagagctt ttaagatctt atgctaaaca gggggtgtcc attatttatg
     4321 tcaatcaaat aggtggtcag gacgatttgc tttttgatgg gcaatcttta gccatggata
     4381 atcaaggtat tatccgtgcc ctggctcccg cctttgaaga aagcttatgt actgtcaata
     4441 tagacggtaa caaaatagac ggatcgatca ccccttatct tgacaaagaa gcacttattt
     4501 ataaggcact ggtttgtggt accagagatt acgtcagaaa aaacaatttc cccggcgtac
     4561 tgctcgggct ctccggaggc attgactctg ccttaacgtt agcaatagct gtcgatgcgc
     4621 tcggagcaga gcaagtccat gctgttctga tgccctcacg atataccgcg tcaatcagca
     4681 atgaagatgc actggaacaa ttaagcaacc ttaaagttgc acattccatt ataccgattg
     4741 aacccatgtt tcaatctatg attgaatcgc ttcatcctgt ttttaaagac ttgccaccgg
     4801 ataccacgga agaaaatatt caggcaagaa tacgtgggat gctgctcatg gctttgtcaa
     4861 ataaaacagg gaaaatggtt cttactacca gtaacaagag tgaaacagct gtaggttatg
     4921 ccactttata tggcgacatg gccggcggat ttgccgtatt aaaagatgta ctgaaaactc
     4981 aagtgtatga attagtgagg tatagaaaca agatttcatt ggttatccca gagcgagtca
     5041 ttaccagacc cccttctgct gaattaaaac caaaccagac agatcaggat agtttacccg
     5101 aatacggtga actggatgcc attatcatgg cttacatgga acagaattta agtccctctg
     5161 aaatcattgc caaaggattt aaacctcaag tagtcaacaa aatcattcaa ttgattaaac
     5221 gtaatgaata caagagacgt caggctgcac caggaataaa gataagtaat ctggcctttg
     5281 gtaaagactg gagatatcct atcactaatg gttttaatca atgcatttga atgcaataac
     5341 tgccttgaaa taaaattcaa ggcagttatt gcatttatta attaaataga tcttatagag
     5401 agactatcat ttggttttga ttgttgtcct tcgtgttgct ccatgttttt cgctctgtta
     5461 ccgaacaagg tggcaatatt ctcatattta tttagattaa ttttaccagg ttcttgtatt
     5521 gcagcctttt cttcttgcct ttcacttaac tggcttctca aacaccgttc aacgtctcca
     5581 aataagcgca aatccagttt tttggcattc gtcgtcttgg ttgcgccact ggcaggaggt
     5641 gttttagatt ttggtccggc agccagacat tcctgcaatt gagctttaaa ttccttcatg
     5701 tcttccttga catcaatgga cttgacagta tttacattca gtaaataagt cttaagttga
     5761 ggaatgtgct cttcctttaa cggatgactt tgatcattta accatgtttt catagatgca
     5821 taggattcta atgaaatttc atgtgcgaaa cctaatgtat ttatccacca caatgaatat
     5881 tcattaggtt taccgcgcaa aacaaaagta gcagaagtcc cattttcttt taatacatca
     5941 ttagtcaact cagcttgagt tctcggatct ttcggattga tgagcagttg aactcgtcct
     6001 aaaagcattt ctcgggcttt tacacactcc tgtttaagct gcttcaaatg caaagcactt
     6061 agattatcaa tatccttaac gatatcctga atctttccgt ctattagaat ttggattaac
     6121 tcttctccgg gttccaaagc atagactttg cctgttgcag ctacccagga aagcttccaa
     6181 atatcacttt caaaaattaa gcaaaattgt ataacaccct tattttcttt ggctgatttt
     6241 ttagctgctt ccaggtcatt ggtaattgaa acattatcga taaatttctg agcactttga
     6301 tgtgtctgta acaattcctg ttttcgagct tctattaatt tttgggtagc caggtattgc
     6361 gacataaact cgtcaattct tttatttatt ttttgtctga gcttcaaaat aattggatgg
     6421 actaactcac tttctaatgg tttatagcca ggaactgact tgataagctc atcaaataaa
     6481 taataattaa acgatccatg ttcccactcc cctttcccaa ccaataactt aaactcctgc
     6541 aagcgagtgt aggcatcagg gaacgcttcc atgcctttaa ttgccatatc aatttcttgt
     6601 ccgcgttttc tggtaaactg gtaaaaccaa cctttaagcc atgattcaat gggcttgtta
     6661 tatcttgccc gggcagattc caagacatga actaattgct tacatccttc atcggattca
     6721 tccgcaattc gacttaataa cttttcggca aattcgttta aattgtcgat tatatccaat
     6781 aactcttttc catcctttgg tggcatatac gctccttgtt ccttgtccaa atttcatctt
     6841 accttaatca gaattttata caagcaacaa aatcattgag ctaactggaa tttaaaattt
     6901 aaattgctca ataaagcgca atagaaaact tgcaaattta agacaaaccg ctaacatgaa
     6961 tcagttaaaa aataataata ataaaaaggg atgtcatgag aattacatta cttacaacag
     7021 ctatattagc tacgggtgtt gcctccgccg cggtacctgt agacgggtgg tatgccagcg
     7081 catttggagg ttacacttat attcccccca acatatacaa tttcgcttat ggggtattcc
     7141 gtgatgaggt gggatacaat aacgggtata acgccggagg acgaataggc taccaaagca
     7201 accccatccg ctacgaagta gaatacacct atctcagggg tgaagccaga aagtttaatc
     7261 ttaactttat cacacagctc ggtgttaccg gttttacctc cgccaatact atcatggcca
     7321 atatttacta tgattgccct gatatgctgc cagccattgc gccatttatt ggattgggga
     7381 ttggttatgc ctctcttagg acagagcttg acagcatagg tccttttaga ccatcttact
     7441 ttaccaccag cgacagcgcc tttgcctacc agggaactgt gggaataact tataatttct
     7501 ccgaaaatta tgcggcaaat ttggcttacc gctatatcgc aaccgataat tcgagcaatt
     7561 ttggaaaaac ctaccaggct catttggcca gcgtgggggc aatctaccgt tttgatcaag
     7621 gaaattataa gtaaggatgc gcaatgaaga agttaactca actctcggta ctatccggtt
     7681 tgctattaag cggttcaact tttgctttca accagcctca aggcttctac gttggaatat
     7741 tgggggaaat aagtcatggg ccatctagtg aggatatcgc gtttctttat gaagatcgaa
     7801 atacatatac gggaacggtc aaatatagtt ctgtttctgg aggaggaggg gctgtattag
     7861 gttataaaat aagtcattac cgtattgaag gggaggtact ttataaccgg atttcaactg
     7921 gccctctaac aataaatacg tgtactttac aaagcccgaa tgtttttact cctactggag
     7981 tttgccctcc tggagtaccc ccaagtggat atgacaattt ccgggtaaat ggcacaggtt
     8041 atgatggcag tagcgccgct ctttatggcc tgctcaacct gtattatgat tttttcagct
     8101 cagtgcatga aagcacttat gtcccttaca ttggtttagg agcaggacaa gtacgcattc
     8161 aaaacagaag caattttgta aaaacagtat taggtcgcct gccgccctct tttggtacta
     8221 gcagtaccat ggttagcgga gcaggacagg gcattatagg ggttgggtat ctcatggatg
     8281 atttcgcctg gcttgctatg gatttccgct ttctcacgac gaatagcctg tctgacttca
     8341 acaacaaacg atatacactc agcacattca atgtgggtgt taactttacc tttgacaagt
     8401 caacctgatg acttcttgtg gccttggggt tcctgccaca aggtcacaaa caaaaccatc
     8461 accatcggcc ctagaaacag gccaagcaaa cccaaagttt ctactccccc caaaatgccg
     8521 aacaaaacag ccaggaaagg taattgtatc gctccaccga taagaactgg ctttacaaaa
     8581 tgatcagcaa caaacataac caaagttccc caaaccagaa caatgatgcc tccaatcaaa
     8641 ctgccaacgg aaagtaaaat caaagcgact atgataaaaa caataggaac aacaaaaggt
     8701 atcatagctg ccaatgcggt aataaaccca gtcaatgtgg gggctggaaa cccaaccaag
     8761 gcatagcaaa tgcccattaa tatacctacc cctatgccaa caacaatagt tccattcaca
     8821 gtacctcgca acgcgctggg gagcctgtcg gaataacgga accatctgtc acccagacaa
     8881 tattctccta tatgctgaat ttgtaatagt aatttatccc catcacgata gaagaaaaaa
     8941 agtgttaaaa gggtaaatcc tacttgaaaa ctacgatgag ccaaattgac cccaatctgc
     9001 ttgatgtaat agctggtagg ggttaacgtt acatgcaaat tagataaaaa ctccttgata
     9061 cttcccggct taccaatatt cacatcccaa tattgaatta aatcatcccc aaccaatgga
     9121 atatttttaa aaaactcagg agcggctcca ccttccttat taatatgctg caaaaaatta
     9181 ataaataatt gagactcctt gataaggatt cctataagcc aactcaaggg aataaggaaa
     9241 agcaagccca tcagtgttgt aaataagaac gctgatgtgt tgtgtttgtt accaaataat
     9301 ttacgccatc tggtgtataa gggataggta gctataacaa taatagcggc ccatatcatg
     9361 gaaggaatga atttatgaac aatataaagc gagaagataa cgatactgac tgttaaccct
     9421 atgctaatta gctctttatg attttcattc attaagcaaa gctccaaacc aggatttgca
     9481 taatacacca ggcaggaact gctgctaaaa catcgtccag cataatccct aacccaccgt
     9541 gaactctttg atcgatcata cgtattggtt ggggtttcca aatatcaaaa atacggaaca
     9601 aaataaagcc aattagcatc catgatattc ctttgggagc taaaaacata gttaataaat
     9661 aaccaactac ttcatcccat acaattcctt tgtaatcatg tatcccaaga tcttctgtca
     9721 ctctgtcact gacccaaact cccagcgcaa atgccagggc agtccataat aaataaatgc
     9781 tccaatgagc attgatcatt aataaataga ggggaatagc agctaatgtc ccccacgtgc
     9841 ctggagcaat tgacatcaat ccactgccaa aaccaaaagc gataaaataa acgggatctt
     9901 gccatactcg atttgccaaa ttgacttggt tcatatttgc tcctcaacct aaatatcgct
     9961 atcacatcac tttagaatcg aattttgtct ttaattttta ttatcatact atagaggcgg
    10021 gatttaaaaa tgtgaatagc ctctgggttg caattcttta cttggactat cagctgattg
    10081 tattcttaat cctgctttct cctctatcac accgatggga aaacagatca gattttcttt
    10141 tttaagatcc cgattaaaag aatccagctg ttgtgttgaa actgtaaaac ataactcgta
    10201 atcatcacct cctgaaagag ccaagtcaag ggcttgttca ggacaatatt gattcaataa
    10261 aggatgcctg ggtattgctt cttcaaataa gttggcccca acaccacttg cgacgcaaat
    10321 atgatttaaa tcagcgccta acccatcaga gatatcaatc gctgaagtag catattttct
    10381 aagcatatgg cataaatcca cccgtggttt aggatgtaat agtttattca ttaattctat
    10441 tttatctttt gcattcactt ttttatgccc atccagcaat tttacagcca gacccgcagc
    10501 ccccaattca ccggaaacca taatcacgtc acccgcttgt gcgccacaac gtctgattgc
    10561 ttgtccctga ggtgtgagcc ccatgatagt caaagtgatt gataacgggc ctttggtcgt
    10621 gtcaccaccg atcagatcca cattatataa gccaagagag tcttttatcc ctctcgcgaa
    10681 ggaatcaagc caactttgat tcaattcggg caaggtcaat gcaagagtcg cccaacatgg
    10741 ctgtgcgccc atggcagcca tgtcactaat attgaccatt actgccttgc aggcaatatc
    10801 atagggatcc cattgcggta gaaaatgaat gcctgaaacc aaagtatccg tgctgactag
    10861 caaatgcatt ccatcgggaa tctgcaagca agcggcatca tcccctatcc caaaaaggac
    10921 atcatttctc ttgcatggga tggatttaaa ataatgatca atcagggtaa attcattcat
    10981 tatcaagact gacttcaaca gagcgaacct gttttgccaa attattcaat accccattaa
    11041 cgtaacgata tccctcttgt gaaccaaact ctttagtcaa ggatactgat tcatcaagaa
    11101 tgaccttata aggtatctca gggcaatgaa acaattcaaa agacccaatt ctcaatacag
    11161 taagctctat tggattcaat gcattgattt ctctgtctaa atacggcagt aaacttgcct
    11221 ccaaagcttc cacatgagtt ggaatgccat ataacaggcg gcagaaatat tctccatcta
    11281 ctttatccat attattaatt gttctgaatt gagcttcaat ttcatgaaga tctgtaccag
    11341 acattagcca ttgataaagg gcttgcaatg caaattttcg agctcgtctt ttaccacgaa
    11401 ttgattgttt ttccacaatt acctcatatt attctaataa caacaagaaa agtgagccct
    11461 tcacattttt tatctaagac ttttccttat cttcattttt catttgatca attgtttgtc
    11521 taaaatcact cacatcttta aagcgtttat acactgatgc aaaacgcaca taggccacat
    11581 gatcaagcct gaataattcc ttcattacca actcgccaac cacctgggaa tcgatctcgc
    11641 gttcacctct tcttcgaatt tcctcagtga tagaaataat agctttttcc aaatcatcaa
    11701 cgctgacagg tcgtttctcc aaggctctca acatgccaga acgtaaatta tcaatatgaa
    11761 acggttctct tcttccatca cgttttataa tcaatggcat aatcaattca gcggtttcaa
    11821 aagtagtaaa ccgctcatga cattccagac attcacgcct tctgcgaact tgagcaccat
    11881 ccgcgaccag acgtgaatcc actacctttg tttcttccgc atgacaaaat ggacaataca
    11941 tgattaggca taaaccggaa attcacgaca gagtagcagc acctgggttt tcactcttga
    12001 aatattagtt tcatcatgaa cattatcaag gacatccgct acccagttag ataacaaagt
    12061 aatctctctt tctttaaagc ctcttgtggt tgcagcaggc gtacccaaac gtaaaccact
    12121 cgttacaaaa ggtgagcgag gatcgttggg aaccgaattt ttattgactg ttatgttggc
    12181 tctgcctaaa gcagcgtcag cttctttacc tgtaattcct ttatttatca aatccactag
    12241 caaaagatgg ttatccgttc cgccagaaac aatatcatag cctctgcttt gtaacacact
    12301 gcacatcgtt ctggcattag ctaaaacctg ttgctgataa gttttgaatt cgggtaacag
    12361 agcttctgca aaagcaactg ccttggctgc aattacatgc attaagggcc caccttgcat
    12421 accaggaaat acagaagaat ttaatttttt ctcaatttct tcgttttcct tacaaaggat
    12481 taaaccaccg cgaggtcctc ttaaagtttt atgagttgtc gtcgtcacaa catccgcata
    12541 aggaacagga gaaggatata accctacagc taccaaacca gctacatgag cgatgtccgc
    12601 cattaaataa gcgccgacct tatcggcaat ttctctaaat cgagcccaat ccaggatcct
    12661 ggaataagct gaaaaaccgg caatgatcaa ttttggtttg tgttgaagcg caagtttctc
    12721 cagagcatca taatcaatca aacccgtatt gctatcaaca ccatactcga cagaatggta
    12781 taatttacct gaaaaattaa cctttgaccc atgagtcaag tgtccaccat gaggtaaagc
    12841 catgcccata aaagtatcgc ctggggacaa taatgccatc ataactgctg cgtttgcttg
    12901 tgatcccgaa tggggttgca cattgacata atgagcgcca aaaagtaatt ttgcccttga
    12961 aatggctaac tcttcagcga catcaacaaa ctcacaacct ccgtaatagc gtttgcctgg
    13021 atacccttca gcgtatttat ttgtcaacac agacccctga gcctccaata ctctcgggct
    13081 gacataattt tcagatgcta ttaattcaat gtgttcttct tggcgtcgtt tttcatcaga
    13141 aattgcttta aaaagaacat catcaaaatt ttttatagta taactttcgt caaacatgaa
    13201 tgttccttgg ttggaaggtt atatttacct tgtttttata ataaggtcat tacgaacact
    13261 ctttacacca tctacgccag ccgcaataat cccggctctt tgttttattt tttgagaatc
    13321 gacaaaaccg ctcaactgca cttgatcttt atatgttttt acttttacgg caaatcccgt
    13381 agtccccaat tggtcaacca aactggcttt caccttggca gtagtcgttg aactatcaag
    13441 atattcccct gtactttccg acagaggaga agcggcacac gcaataatta tcatggatat
    13501 ggatcccact aataacatct taagtgagtc gcgcatagag gctccttaaa gataaaaacg
    13561 ttattagagt atcataaaaa ttacccggat acgaagagga taatagtaca attttagttc
    13621 ttttaaaaaa aaagtttcct gtattcagca agttaaatta ttaactatgg ccatgaattt
    13681 ttttctgttc ctcaggattg gaagtatcat gtccatgtac attagaagaa tcagggtggc
    13741 tgtgcacatt accattatca tgagcatgtc catgcacctg accgttattt gcattgtgtc
    13801 cgtgaacatt tttctgaact tcaactctgg cttgtgatgg tggcggagta actaccgcag
    13861 catggccttg agttttcgca tgctcataat gaaccttccc ccttggcgca ctgtagtatc
    13921 gattattact atgatatcgc tgattgtaat gacgatgata atgcccttga ggataagagg
    13981 gggtaacctc tacgcgagga gcaggcccca aatactctgg ctgataaaag tcttcttgta
    14041 taactgtgca ggaagaaagt aagggaaaca atactacgca gggaatgagg tatttcatgt
    14101 acaactcctc aaaggaaagg aaaactctcg aatcattgag agctttcccg aattttcaat
    14161 gaattatctg gttgataact cagccttagc ttctttcaag taaggaacta cacggatggt
    14221 actgttcaca tttgtccaac cggaataaat agtatagtaa ggtgatgtaa tagtaatgta
    14281 cataccacta tggcaataga aattatagaa taggcttatg tagtgaggtg actcataacg
    14341 atagatatta aaatctatag ttgtgccatc atcaaacgta ccgaatacac gaacatctgt
    14401 gaagctttgg ttaatgattt caatttggca gtaaccagga taagttacgc tcttttgtat
    14461 tgatttcttg tctgcattat cagccttggg atgtaagtga agatttgcag caaaaacgtt
    14521 agaaaataaa ctgaaacata caacaaataa caatgatttt aacttcatga gcttcccctt
    14581 agtaagatgg aagctgcata gtatcaaagt attcaattta gtcaatagga tttttttgta
    14641 cccataaaat caaagttcat tacaattcaa ttttgttaga gaagaagcag aaacaactat
    14701 gaaaatatta aaaataacaa gtcatctcaa acaataagat gataaagtaa atgtttatgt
    14761 tatatttgct aaattaaaat ttttttcaag cattaacagg ggaagataat gaataaacga
    14821 ttgactatca tgggaataat cttgttggta gttttcggtg gaatcatagc ttttaatcta
    14881 attaaggctt acatgattaa acaattcttc gccagttatg cgcctcctgc tgttaccgtt
    14941 tcttctgctg ttgctcaagc agtggattgg aagccaacca ttgacgctgt aggtaatttc
    15001 gttgcaatca acggagttga tgtcaactcg gaagcatctg gcaatgtagt tcaaattcat
    15061 tttgaatcag ggcaatacgt agaaaaagat gagcccttga tcaccattga cgacagcatc
    15121 gatcaagcct tgctcaaatt taaccagtcg gaattaacgc ttaaagaact cagctataaa
    15181 cggcaaactg atctcttcaa gcgtggcgcc acgccaagct ccagtgttga tgaagccaaa
    15241 gctaacttgc aacaggctca ggcgaaagta gaacagattc aagcgcaaat taaacaaaaa
    15301 cacataggag cccctttttc cggccgcctt ggcatccgcc aggtaaacct gggccaatac
    15361 gtcactccag gacaaacctc catagtttct ttgcaatcac tggatcccct ttacctggaa
    15421 ttttacttgc cagagcaata ttacaaaaga attcatctta accagcctgt gacctttcgc
    15481 gtagaggaat ttcctaatat tctctttgaa ggagtcattc atgcagtaaa ctctaaagta
    15541 gatctaacta ctcataatgt tcaggtacaa gccctattac cgaactgccc tgctgatgca
    15601 attcaggatc caaccaaatc tcctcttatc aaagtacgta aacaagtcag aggagataaa
    15661 ctgattgtgt cctgcagtac agaattaaac aaacaaaata aaatacgaaa ttttgtattt
    15721 atcccaggca tgtttgcttc cattgaagtg gatcaacctg cagagcctaa tactattatc
    15781 gtcccttcca cagccatttc ttacagcctc tatggaaatg ctgtttatat cattgaaaaa
    15841 agtaaagaag gtaagaaaaa taaagatggt actgaccagt tggttgtaaa ccgtgtattc
    15901 gtcactaccg gggagcaaca gggaaattac acagttatca agaaaggcat taaagtaggt
    15961 caattggtcg taagtactgg tgacttgaaa ttacaaaatg gtacccctgt tgttattaac
    16021 aacagtgtca aactcgatac agacagtgat cccaaccaac tgggacagta gatcgtgttg
    16081 gcaacctggt atttaaatca ttttataaca ttggcatgct ctaataatat ggcttgttgt
    16141 tctttactac cgactaggat catggtcagt tttttgcagg ataacttatg aaattcacag
    16201 atctattcat caagcgcccg gtactctcca tagtggtcag tatgttgatt tttctattcg
    16261 ggataaactc catctataaa atgcaaattc gccaataccc taaaatggag aatacagtca
    16321 taactataat gactggttat cctggcgctg atgctgaact catagccgga tttatcacta
    16381 ctcctcttga aagtgctgtt gccagtgctg aaggaataga ttatatgact tcatccagtc
    16441 agcaaggcct tagcacaatt accttaaaca tcaagttgaa tttcgaccct caagttgctt
    16501 ttactgatgt catgagtaaa gtgcagcaaa cgctcaatca gttacctaaa gagtcacaac
    16561 aaccggtgat attgaaaaaa tcagactcat caaccgcatt gatgtacatt agtctggaca
    16621 gtaaggatat gactcctcag caaattactg actacgctac ccgagtcgtt caaccccagt
    16681 tgcaaacagt ggatggagta gccaaggttg atattttagg tggtcaaact tattccatgc
    16741 gaatattcat ggatccaatt aaaatggctg cgcttaacgt cactcctgct gatgtgtcct
    16801 ctgttttagc aagtaacaat ttcttgactg cggctggaag tactaaaagt gaatacgtag
    16861 ccatcaatgt cacggctaaa actgatttga acaatgtcgg cgaatttgaa agactgattg
    16921 taaaaagcga taaggggtca ataatcaggc tcagggatat cgctaaagtg gaactgggtt
    16981 cacaaaatta tgatagctca gtgactttca acagcaaaaa ggccgtcttt atatcgatca
    17041 ctccaacacc aacggctaac cctctcacag taatcaatga tgctagaaaa ttgtttcctt
    17101 cgatagtcag agaattcccg ccgtcattaa ctggcacaat agtatacgat gcaacagcat
    17161 tcattcgggc atctatccat gaggtaatgg aaactatcat tgaagccgca gtcatcgtga
    17221 tcatcgttat ctttttattt ttaggctctg ttcgttcagt tcttattccc gttgtcacca
    17281 ttccgctgtc attggttggt gtctgtactt tgatgctcat actcggttac agcatcaact
    17341 tgcttaccct tcttgccttt gtgttagcaa taggattggt agtggatgat gctattgtag
    17401 tggtagaaaa cgtacatcgc catttggaag aaggaaaaac cccctttgaa tccgcaatta
    17461 taggcgccag agaaattgct accccggtta ttgcgatgac aatcaccctg gcagctgttt
    17521 atgcaccaat agggtttatg agtggactga ctggcgcttt gttcaaggaa tttgctttta
    17581 cgctggcaag tgcggttatt atttcaggga ttattgcctt aacgctgtcc cccatgatgt
    17641 gttcaaaaat tctatccgaa gaagccacta gtggaaaatt tgctattttt ctggatgagt
    17701 tttttaacaa actgaaacgc gtttaccaga atgcacttca tagcatattg gatactcgag
    17761 aaataatgtt gctattagcc ggggtcgtga ttttaatgtt gccctatttg tattcgaaca
    17821 cagccgctga aaccgccccg gatgaagatc aaggcttctt ttttgtactg gctactgcac
    17881 cacaatacgc taccctaaac tatatcgaag cctttacaaa accttttgat gccatttata
    17941 aaagttttcc tgaaaccgaa aactatttta ctgtaaacac gagccaacct atttcaggaa
    18001 tggttttgaa accatgggat caaaggaaaa aaagtcagtt tgctctaaaa caacctttac
    18061 aaaacaagtt atctaaaatt gcaggactta atgctttagc cattgttcca cctcccctgc
    18121 ctggcggtgg tggtggacct gccgttcagt ttgtggttaa gacaacgaat gatttccaga
    18181 gtttaatgga tgtgtcgaac cagctaactg aaaaagcccg gaacagtggg ctatttattt
    18241 acatagacaa ttctcttaaa tttaaccaac cacaagtgga atttagcatc aatcgagcaa
    18301 aagcttctga aatgggcttg gatatgagag cgcttggaag caccctgaca agtgctctat
    18361 ctaacaacta catcaattat tttaatctgg aaggccgaag ctaccaggtc atcccacaac
    18421 tggctcgcaa attccgtctt actattgaac agataggcca aatttatgta aaaacactta
    18481 acggaacgat ggttccatta tctactgtga taaccccaac cgaaaaaacg caacctaatg
    18541 cagcgactca tttccaacaa ctcaattcag caacaattca ggcggtcatg atgccaggca
    18601 agacattggg tcaaggattg gagtttttac aaaaagcagc ccaggaagtc ttgcccaaag
    18661 gatttacata tgattatgga ggacagtcca ggcaatttat gcaagaagga agtgcgctga
    18721 tatttgcatt cctttttgct attattgtta tctttttagt gctggcagcg cagtatgaaa
    18781 gttttaggga tccattaatc gttctcatca gcgtgccaat gtctatctgt ggtgcgctta
    18841 tcccgctcaa cttgggagca gccacaatta atatctacac tcaagtgggt ttgattactt
    18901 tgattggact catcagtaaa catggaatat tgatagtaga tttcgctaat catttgcaac
    18961 gtgaaaaaaa tcttgataaa cgtgctgccg ttgaggaagc tgctggaatt agattacgac
    19021 ccatattaat gactacagct gccatggtgt ttggagtaat acctcttctt attgccagtg
    19081 gtgctggagc agcgagtcga ttcgacatag ggctcgttat ctccacaggg ttattaatag
    19141 gtacctgttt cacactgttc gtagtaccta ctttatatac ctacttagct gaagatcatc
    19201 gacataagcc aaacgaacca gcagagatcc agtaattcga ctttggattt tttcgtaagc
    19261 cttattaatc tttaaataat tcgtcaaaca gcctgcatgt ttcctcttct ccgaagcatg
    19321 cagccgcact ttgtggatac cagcgacaag caacggtcat tagagaatcg gccatttaaa
    19381 actcaaagga ctataatgat tttttcctga gttcctgttt ttttaaaatg gctaaattcc
    19441 catactctat gataaaattg tgagttttta ccagtatagg ctatacgata atggacaaaa
    19501 cttactctcc ggaagcaata gaaaaagccc tttataaaaa atgggaaagt catcattact
    19561 ttcaaccacg cggtgagggt aaacgatttt gtattatgct tccgcctcct aatgtaacag
    19621 gaagcctcca tatgggacat ggttttcaac acaccataat ggatgcatta accagatatc
    19681 atcgcatgct tggtgacaaa acattatggc aaccaggtac cgatcatgcc ggcatctcaa
    19741 ctcaacttgt tgtagaacgt caactggaag cccaaggcat atcgagaaaa gatctcaccc
    19801 gtgaacaatt tctggataaa gtgtggcaat ggaaagaaga gtccggtaat accatcacgc
    19861 aacaaatgag gcgtttaggt gcttccgtag attggagtcg agaacgtttc accatggatg
    19921 aaggattgtc tgctgctgta caaaaggtat ttgtacagct ttatgaagaa ggacttattt
    19981 accgaggaac ccgcctggtg aattgggacc ccaaactggg cactgctgtt tcagatcttg
    20041 aagttctctc tgaagaagaa gatggctttc tttggcacat tcgctatcct gttgttgatt
    20101 ccgaagaatt tctaatagtt gccactacac gccccgaaac cttactcggt gattgtgccg
    20161 ttgccgttca ccccgatgac tcacgtttca ggcaccttat tggaaagcag gttcatctgc
    20221 ccttatgtga tagaacaata cccgtgattg cggacgatta cgttgacaag gaatttggca
    20281 gtgggtgtgt aaaaattacc cccgcacatg attttaacga tcatgaagta ggcaaacgtc
    20341 atcaattacc acaaatcaat attttaacaa agaaagggac tatcaataaa aacgctccct
    20401 taaaatatca ggggatggat agatttgttg cccgagaaca aatcattaaa gatttggaac
    20461 aggaaggctt gctggcgaaa acagaacctc acaaattaaa agtaccccga ggtgaaaaat
    20521 cgaatgtcat catcgaacct ttattaactg atcaatggta tgttaaaacc aaaccattgg
    20581 cagaacctgc tatcgccgct gtaaagaaag gagacatccg ttttattccc gagacatggg
    20641 ataaaaccta tttccagtgg atggataata ttgaggactg gtgtatcagc cgccaactat
    20701 ggtggggaca tagaattcct gcgtggtatg ataatcatgg taatatttat gtaggttata
    20761 gtgaaaacga tgttcgcttt aaacataaaa ttgatcaatc tactcccctg aaacaagatg
    20821 aagatgttct ggatacctgg ttttcatcag ctctctggcc cttttcaaca ttaggctggc
    20881 ctgagcgtac tcccgaattg gaacaatttt accctacttc tgtactggta accggatttg
    20941 atatcatatt tttctgggtt gcccgtatga tcatgatggg actaaagttc actgggaaaa
    21001 ttccctttaa agaagtcttt ataactggtt taattcgtga cagtgaaggg cacaagatgt
    21061 ccaaatccaa aggaaatgta ctggatccat tggacattgt agacggaatt gatctggatt
    21121 ctttaatagc caaacgaacg tctaacctga tgttaaattc tgttcgtgac agaatcacaa
    21181 aagccactcg taaggaattt cctgagggaa tcagcgctta tggaacagac gcgctgcgat
    21241 tcacttactg ttctcttgct tcaacagggc gtaatgtgcg ttttgattta ggacgagttg
    21301 aaggatatag aaatttttgc aataaactat ggaacgcagc tcgctatgtc ttattaaata
    21361 cagacgaaga gcaaattgat tttggggatg gcgcatttca atacagccca gctgatcaat
    21421 ggatactatc ccggctacaa aataccgtaa gtaaggtaca tcactatttt gaaacctatc
    21481 ggtttgatct cctcgctaat acgctatatg aatttgtctg gcatgaatat tgtgactggt
    21541 atttagaatt atccaagccc atattacagg atgaacaggc tttaagcgcc atgaaaagag
    21601 gcacgcgaag aactttaatt catgttctgg atcaaatatt gaagcttctt catcctctga
    21661 tgccatttat tacagaagag atttggcaaa aaaccaccaa atttaccagt gaaaatggga
    21721 ttagtatcat gctgagcaca tatcccaaag tcaatgagga attcataaac cccgctatag
    21781 aggaagaatt ggattggtta aagtcagcca ttcaatcact acgaaccata cgcagtgaaa
    21841 tgtctatctc acccgccaag ttaatacctt tatacatacg caatattact cctgaattaa
    21901 aggagcgtat cgcaaaatat gaaaaaattc tcaagacgct aagcaagata gacaaaatta
    21961 attatcttgc tcctgatgag aaagttcctg tttcagctac tgcggtattg ggtgaaattg
    22021 agttgttgat tccaatggca gatttgattg ataaggaggc agagttatca agactgaata
    22081 aagagcttgc caaattgaat aaagacatcg agctagctca gggtaaatta aataacccta
    22141 aattcactga taaagcgcct gaagaaatca ttgctaaaga aaaagacaaa ttagctcagg
    22201 ctcaactggc taaagataaa cttttgcaac ataaaaacag gattgagtca ttataactca
    22261 atgatcctta gttaaaccct ccttacagtc atggctcaac ttaactcatt attctatacg
    22321 tgactgtaag gaacaaatgg cacaaaccgg attatttaaa agaaaggatg tcttctcgtg
    22381 gtaactcaat tcaatacgct taaatcctat attttcccca tcattttgct attatctatt
    22441 ttacttggtg ggttaacagg atatgttttt aatcaaaacg ttgcctggtt aaaacccttt
    22501 ggcgatattt ttcttaattt gatttttact accatcgttc ccctaatttt tttcagtgta
    22561 tcctcagcga ttgcaagaac tggttctatt ggcaaattag gcaaaatatt ctactcaatg
    22621 acagtcatat tcttatttac aggaatagtt gctgcagttt atgctctttt tgtagtttta
    22681 cttttccctc cagcgcaaaa catgcctgtt ctcttggaaa gttcaaagca actctctccg
    22741 acaaatctcc cggatcgctt gatagagatt tttactgtac ctgagttttc aaaattattc
    22801 tctcatgagc atattctggc attagttctt ttttcaattc tggtaggatt ggctgtagcc
    22861 cattccaaag ataaaggtac aatttttaat tccttcttaa aagcaggaga agacgtattc
    22921 atgtcagtat ttcgcctgat tatgtattat gctcctatcg gtttttttgc ctattttgca
    22981 gtaatagtaa gcgaaattgg cccccaatta atggaaaatt atttacgcat tgccatactc
    23041 tactataccg cttcagtgtt ctactttttc ttcgccttaa ctgcctatgc ttatttggcg
    23101 ggaaaatccc agggtgtccg gctattctgg ggaaatattt tccttcctgc agcgacctcc
    23161 atagcaactt gtagtagtgc cgccagtatt cctgccaact taatggcaac caaatccatg
    23221 cgcatccctg ctgaaatcta tgaaacatca atacccttag gctccataat tcataaagat
    23281 ggatcagtca tgggcggtgt ttttaaaatt gcctttttat tcgggatctt tcatttacct
    23341 tttaccagct tgcccgttct tatgatggca ttgggggttt ctttgttggt aggaacagta
    23401 atgggagcga ttcctagtgg cgggatgtta ggagaattgt taattttgtc agtgtatggt
    23461 tttccacctt ctgttttaat tgcagttgct gctattagca tcatcattga tcccatagcg
    23521 acaatggtca acgtaacaag caatagtgtt tgcagcatga tgattgcacg tctggtcgaa
    23581 ggtaaaaatt ggtttctcag atcctccgat gaaaataacc tataatctaa ggtatgttta
    23641 atataaaaag cacatattaa attttcatta tcccatatgt gcaatataaa ggcatttttt
    23701 ggagggaata gtaatgaaaa aaatactgta tgttattccg ggtattttat taattacgaa
    23761 ctcttttgcc cttgatatac ctgggaatat taccaattct aacaaagatc tgctcccttc
    23821 accttttcct gtttacgtca ttgaggggtc tgctgttgtc aatcatcctt acccgggagc
    23881 aaaaaaagta ctcctgccaa ccgacaacgg ctatgtagat tatccaggat gctatattgc
    23941 ttgttattcg cataatacag gtgtctacgc catttcccca acaatttcag tcatgggaca
    24001 aattcgagtc aaagggcaat atgacgcccg aatttgccaa cctgatggat ataaaaatca
    24061 agacatcagt gctgcttatc aatttaaaca gctttgtgct gaaaagatcc ccgcctgcca
    24121 aaataacagt tgctgggccg gtggcgatac tggcggatgg tttggaattc aataagcaga
    24181 ccaatgaatt tcacagaata aaatgagata ggatacttga ttttgggtca aagccacgcc
    24241 tttaaaattt ttagcaagca tcatcactct caacttcagc agagagataa ttcagcagtt
    24301 tgaatagtgg gggatacgga gatatttggt gaatacctca tattaattaa attagtaatt
    24361 aaccagtgaa ccaataacat acttacacag gctattatag ttaaagggat tggagttgtg
    24421 atcaacagaa aaaaaccaat aggtaaaaaa acgctattaa gaattatttt atttaaatct
    24481 ttcatacgag cgttcgtcat tgccaattgt tcattcttga tttgttctcg actagtctca
    24541 ttaagcttaa gttgatgtat taattgccta tctttctcta tatcgattaa aactcgtaaa
    24601 agtgaactaa taagaatgat agaagatatt acaggcgaaa gtggtactgc cactcctgaa
    24661 aatgaaaata atccaaaagc tatcactaca gataagcttg tgagcaagga tcgaagacca
    24721 tttaattctt tattctgcca ttcaattgct aaacgagagc gctccatacc catagcctga
    24781 ttgtatttta agtcatattc ctttttgtct tgttggtaac gaataatcat cactaaaata
    24841 tcgatgagtt gagccaacgt ttctaattgc atcccttgaa ggcctgcaga tattgagttt
    24901 ctataagata accaaaaaaa ctgggtcagg ttaacaacgc accacaagct atcatttaaa
    24961 agactgtagt aaatatccct tcgtatttta tgattagttt tttcttgcga ttcgactaaa
    25021 atgagatgcg ctaataaacc aacgttagct gaaaatcgaa gcatatacag agaaaagctc
    25081 atcacaccca aagccgcatc taagggagca agatcccttg tgtttttttg tgattttgct
    25141 aaatactttg ctaaaccttt accacgattt aattgaagtc ttttgtcatt taaatcagtc
    25201 agtttgctat taaaaaacat taatgtggat tttatgatcg gttttgctgt gcctgactcg
    25261 tttttgctca tattagtatc atca
//
LOCUS       DATNSN010000030        25141 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.030, whole genome shotgun sequence.
ACCESSION   DATNSN010000030 DATNSN010000000
VERSION     DATNSN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25141
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.030"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            2..877
                     /locus_tag="VAH76_001042"
     CDS             2..877
                     /locus_tag="VAH76_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEO1385689.1"
                     /translation="MPLIIFPKQGIECYVKRDDELGCGISGTKIRKYSSLIPFLKIKG
                     IRHLIIIAGAQSNNLLAALQVARECQLKVTAFLVKPKHLKIQGNFKLSLLFLHENEII
                     WINREEWYRVNEFAEQYLEGLRETGYILSEGASVKESMKGAMSLANDIKENEKILGFA
                     FDHIFVDAGTGFSAIALIKGFHELQHKGFIHVLLLADSEEVFKKKLMHWAGVNPDNYA
                     CFYPTTAKSFGSVNQTIKQEIKRLAYEEGILADPIYSAKLFYEARRYIEKYELKGKIL
                     IVHSGGILTMPGFAF"
     gene            1090..2667
                     /locus_tag="VAH76_001043"
     CDS             1090..2667
                     /locus_tag="VAH76_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase Lgt1"
                     /protein_id="HEO1385690.1"
                     /translation="MKARRDQQLSKLRMRFFSALNHTSEIDLHVLFNDLKSILTLDSI
                     KHLKEGSVAYAIIQELLKQDDAQNKIQSFLHGAIKNVIHPGVIKGLTPDEINWNVAKA
                     YPKYYEHEEFPDVTFGGFKVRDSNEFKFKTNIQTSIWFSIKPDLFMPSKQQEALKRRR
                     EQYPGCEIRLIYSSSLLNAEANRQMKAFARKQNISLIDIDSVKTNSPLYPLLKSELAH
                     LGKGGNPAAASDLCRWIPEVFNEGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSII
                     SEPIAPHHRRQEAVCMNTDIIAYSNDKRTQKMMDTVARHLKNIYDDPYTALKDTPLAQ
                     TAFFNKCQEERKSIFDLRKGLQDAFRSDSLLQLYDFLGADKFKEVFKLKEAQSKYINE
                     HISEFSEKDLLLNLISDKPSEINQHTLDFVKAKAMYIDIAKEHYSAFYKPLVEEISGP
                     GVIYNALGGAGSFTTTHRRLTGPMLPTTPPRVLQVFCDAHDKGPFVSDNIARWQTNVR
                     DLGVLNREGLSWLPSVG"
     gene            2870..4741
                     /gene="htpG"
                     /locus_tag="VAH76_001044"
     CDS             2870..4741
                     /gene="htpG"
                     /locus_tag="VAH76_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEO1385691.1"
                     /translation="MVSKQQTMGFQTEVKQMLHLVVHSLYSNKEIFLRELISNASDAL
                     DKLRFLALSNGSLFENDSDLKISIQINEKLQTITISDNGIGLSWEEAVENLGTIAKSG
                     TKEFISQLTGEQAKDSQLIGQFGVGFYSAFIVADKVTVKSRRAGLQPEDGIVWESKGD
                     GEFTIGYEKKSTRGTEITLHLKPENDEFLSDWRIRGIISKYSDHICWPIVMKKLSEEG
                     KESKEFETVNKATALWTLQKSEISEEDYKQLYKHISHDYMDPLTWSHNHVEGKHEYIT
                     LLYIPAHAPFDLWQHEAKHGLKLYVKRVFIMDEATQFLPRYLRFVKGIVDASDLPLNI
                     SREILQDNKQVESIRAACTKRVLSMLEKMATNDKETYQKFWNEFGLVLKEGPIEDFAN
                     KEAIAKLLRFSTTASGSEKQEVSLEEYISRMKEGQDKIYYITASSYNAAKNSPHLEIF
                     RKKGIEVLLLSDKVDEWLVGYMNEFAGKKLQSISKGKIELGDDETSEQIKEQEKTLEP
                     LIKHIKSVLNERVKDVLLTNRLTDSPACVVADEQDMGLEMQRILQAAGQQVPVSKPIF
                     EINPDHALIKRLHDIQDDNQFELWVTMLFEQAVLAEGGQLDNPADFVNRVNRLLVSS"
     gene            complement(4853..5149)
                     /locus_tag="VAH76_001045"
     CDS             complement(4853..5149)
                     /locus_tag="VAH76_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEO1385692.1"
                     /translation="MNVIDTQATTQSTKQEQGLQDLVYSLVTRFLAENKSKSINDLYD
                     MILSEVEPPLLQAVMEKRRGNQLQAAKMLGISRGTIRKKLQRYFGTKYFRLTEE"
     gene            complement(5265..6419)
                     /gene="lpxB"
                     /locus_tag="VAH76_001046"
     CDS             complement(5265..6419)
                     /gene="lpxB"
                     /locus_tag="VAH76_001046"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEO1385693.1"
                     /translation="MPNASRVVIVAGEESGDHHAAELVKQLKAVYPDLEISGIGGKHL
                     RAAGVHLISDLTRYAVTGLTEIIPFLKIFRKAFQDIKQHLSTQKPDLLILVDYPAFNL
                     RLAKYAKKKLGLKIIYYISPQIWAWKGKRIHLIKDCIDKMAVIFPFEKTIYENAGVPV
                     SFVGHPLVKKIAAAKDKHSSRTSLGLPLNEPIIALLPGSRHSEIERHIPILVNTAKLL
                     TLDSPKLRFVVPIAGTINPDKVKAYFSNQNLTVTFIQGQAIECMSAADFVIVASGTAS
                     LECALLEKPMCIIYKSSFLTYVAAMYFIKVKFLGLCNLLANKMMVPEFLQYDCNPIEL
                     SRYISNFHNDPNQPKSMINQLAKLKESLSSSQADCSLFDLVVAELPEKNA"
     gene            complement(6406..7368)
                     /locus_tag="VAH76_001047"
     CDS             complement(6406..7368)
                     /locus_tag="VAH76_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO1385694.1"
                     /translation="MNKIRCAVIGVGYLGRFHAQKYQLIPNAELVAVCDVNSTVCEVV
                     SSELNVPAYIDYHDLLGKVDAVSIAATTNKHFEIAKFCLQHGIHVLIEKPITETVSQA
                     EELIRLAKQHQVKLQVGHLERFNSARLALDEYLELPLFIESQRLAPFNPRGTDVNVIL
                     DLMIHDIDIIQNIVQSPIKSITAQGAPVLTKAIDIANARISFANHCVANVTASRISFK
                     TERKTRIFQENSYISIDYQNKQLAVFKKGTGEMFPGIPDITRDQREFEKGDALLEEIK
                     AFLNCIENDSVPLVTGEEGRDALKTAEQITALINNNLVERYAEC"
     gene            7589..8164
                     /gene="rnhB"
                     /locus_tag="VAH76_001048"
     CDS             7589..8164
                     /gene="rnhB"
                     /locus_tag="VAH76_001048"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO1385695.1"
                     /translation="MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKL
                     SPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVVV
                     DGAFTPQLNIPCKAIVQGDSLVPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
                     ATPVHKEALMRLGPCRIHRRSYSPVADLISK"
     gene            complement(8258..9376)
                     /gene="rodA"
                     /locus_tag="VAH76_001049"
     CDS             complement(8258..9376)
                     /gene="rodA"
                     /locus_tag="VAH76_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO1385696.1"
                     /translation="MSRQTTKPVYRFTAKSLHLDFPLLGLILTLIAFGLLILYSASNA
                     NMGMIMRQSMRLLFAFLIMFVLGFIPPHKYKIWTPWIYGVGLSLLIAVMLMGKIGKGA
                     QRWLELGLFRFQPSEIMKLAVPMMAAWFFDRQSHPSSIRSIGIASLIIFIPALLIAKQ
                     PDLGTAIMVTVAGLCVVFLAGIRFKIILLIALLMCSAIPVVWNLMHDYQKQRVYTLID
                     PEQDPLGAGYHIIQSKIAIGSGGLMGKGWLKGSQSHLNFLPEHATDFIFAVSGEEFGF
                     AGGFAIVALIVLISLRSLNIANNAQTTYTRLLSASLAMTFFLSAFVNIGMVMGIIPVV
                     GIPLPLVSYGGTAMVTFLASFGILMSISSHRILFNSLN"
     gene            complement(9373..11226)
                     /gene="mrdA"
                     /locus_tag="VAH76_001050"
     CDS             complement(9373..11226)
                     /gene="mrdA"
                     /locus_tag="VAH76_001050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO1385697.1"
                     /translation="MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQI
                     SEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTL
                     TKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVSIK
                     ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGK
                     VGYQMVETDVSGRTLRIINKVNPHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSR
                     NGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVG
                     LAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQL
                     GNKMGISNIEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAI
                     GQGFMLATPLQMANATASLSQHGQRFRPHLLIKTVNSDTNETEEYKPFEEYPVSLRDE
                     ANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKYEDIPEH
                     LRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTI"
     gene            complement(11393..11863)
                     /gene="rlmH"
                     /locus_tag="VAH76_001051"
     CDS             complement(11393..11863)
                     /gene="rlmH"
                     /locus_tag="VAH76_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO1385698.1"
                     /translation="MLKITIITLGNKMPDWVNSGVNEYAKRFHDGIQIKLIEIPLLRR
                     NKSSDLARILEKESALIKDALPANARLIALDMLGKSFSSEELALKLTQLQQISSHLCF
                     IIGGPEGLSNEILSLCDERWSLSKLTLPHPLVRIILLESLYRAWSIINNHPYHK"
     gene            complement(11872..12210)
                     /gene="rsfS"
                     /locus_tag="VAH76_001052"
     CDS             complement(11872..12210)
                     /gene="rsfS"
                     /locus_tag="VAH76_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO1385699.1"
                     /translation="MLEKNPTLEKLLKSLEDIQAIDIKIIDVHKQTTITDFMVITSGR
                     SSRHVKSIAQKVLEDMKVAGLPCLSSTGVEHGDWALIDFGDIILHVMQPEFRHFYNLE
                     GLWEEHPGNS"
     gene            complement(12316..13758)
                     /locus_tag="VAH76_001053"
     CDS             complement(12316..13758)
                     /locus_tag="VAH76_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEO1385700.1"
                     /translation="MEKHPKSLRIYFTTEMWERYGFYAVQSLLALYLALHFKWPDKEI
                     YSLVGSFTALTYLSPLVGGWIADKLIGQKRAILLGAIVLFLSYCMLSLIDNSLALTSS
                     LAGVAVGTGLLKPNISSLLGNEYPVGSTKRESGFTIFYMGITTGIILGTTLPSKFSEY
                     FGWSASFTSASIGLIIAFVVFLFGIHQYKIKDYNPFVFQPGKIISAFVLLVFLWSLSF
                     YILSSPQLANIMFGLVVLFSASYILYSVNRESANQSRQTLVIGLLCIISVIFWAFYFQ
                     MFMSLTLFIARVVEPSFLGMDFPPPYYITVQSIGMLIIGYFLAKRHHNLTMIERGLST
                     GKKFVLAMVFMTLAYSIIAFVSTVVDKSILISPLLIIPAYLMISLAELLLSPVGLSAI
                     TVLADKNKVSTMMGIFFVSLGIGGFLSGKLADLTAIPTGETNIIVLKTLYATAFTQQL
                     SILFIATLGCLVLFAVIKFLLTHIQICEKQ"
     gene            13981..15645
                     /locus_tag="VAH76_001054"
     CDS             13981..15645
                     /locus_tag="VAH76_001054"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEO1385701.1"
                     /translation="MSKDKNKRVISAPRGTEIQAKNWLTEAALRMICNNLDPNVAEDP
                     DSLIVYGGLGKAARNWECFDEIVHVLTLLNNDQTLLIQSGKPVGVFTTHEEAPRILIA
                     NSNLVPRWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAAAKKHYQ
                     GDLSGRWILTAGLGGMGGAQPLAGTMAGASVLAVECDRQRIEKRLQTKYLDRYTDNLS
                     EALDWINESCRCKKPVSVAVLGNAAEIFPQLVKLGVHPSLVTDQTSAHDPLNGYLPLG
                     WTLEQAVEMRKKSPEEVVDAAKKSMAVQVHAMLEFHNRGIPVFDYGNNIRQMAFEAGE
                     KNAFSFEGFVPAYIRPLFCEGIGPFRWVALSGDPEDIYATDERVKQLIPDDPHLHHWL
                     DMAREKISFQGLPARICWVGLKDRARLALAFNEMVKNKQVKAPIVIGRDHLDSGSVAS
                     PNRETEGMLDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGVVIVADG
                     TEKAAKRLARVLHNDPATGVMRHADAGYQIAKQCAKENSLWLPMES"
     gene            15659..17176
                     /gene="hutH"
                     /locus_tag="VAH76_001055"
     CDS             15659..17176
                     /gene="hutH"
                     /locus_tag="VAH76_001055"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEO1385702.1"
                     /translation="MSEHFILQPGQLSLLSIKQILDEELSCVLAENAFELIRASHQTV
                     KKVIDEKKTVYGINTGFGSLANQTISSDCLKELQRNIVLSHACGTGKLLPDDVVALIL
                     LLKINNLSQGYSGVRLQLINALIALFNHKVYPCIPSKGSVGASGDLVPLAHLSLPLLG
                     EGEVRHQGQVISAEEGLKLAGLKPLELEAKEGLALLNGLQVSTALALSALFISETLFE
                     TAIISGSLSVDAASGSDVPFDDRIHQIRGHQAQISAARMYRNLLAGSQIRESHRYCNR
                     VQDPYSLRCQPQIMGAVLHQMEFVGQTLQVEANAISDNPLVFAEQGDILSGGNFHGEI
                     IAMAADNLALALSEIGGSAERRIALLIDKNFSGLPAFLVRESGLNSGFMIAHVTAASC
                     ASDNKALAHPHSVDSLPTSANQEDHVSMATSAARRLHEMIDNTSTILAIELLAACQGL
                     EFHKPLKTSPQLDKIYQSVRSVVKEYDKDRYFAPDIEKIKKKILDKEFSLLTLTN"
     gene            17193..18581
                     /locus_tag="VAH76_001056"
     CDS             17193..18581
                     /locus_tag="VAH76_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEO1385703.1"
                     /translation="MELMAEFLALHQQHKNNPYPSLSERKKLLIAIKKILQTEAYTLA
                     EAINKDFTHRPVEETLFLEIFPTIKAINFCLKNMKKWMRKRRRNVSWLFIPAKAYVIP
                     QPLGVVGILVPWNYPVYLALVPAIYALASGNRVMIKMSELSPHIGDTLQKLVHASGIN
                     HSISIINGDIELSKHFASLPFGHLMFTGSTNVGKLVMKAASDNLTPVTLELGGKSPAI
                     ISPTMNPAYFKRLFMGKLLNAAQTCIAPDYVLIPKGWEERVETEFKKFINTFYPDLMS
                     NEQYSSIISESHKKRLLDLVEDARSKGARVVEFGHLIPNSSKLPVFLLFDITNDMLVM
                     KEEIFGPILPVLTYNSIIDAVDFINSFPNPLALYYFGEDKNEIKIIQTTTLSGALTIN
                     ETLMHIAIDDLPFGGVGHSGMGHYHGKEGFDTFSKLKPVLVQRFISTVSWLYPPYGAF
                     MRMFLAWVGGIK"
     gene            18593..19402
                     /locus_tag="VAH76_001057"
     CDS             18593..19402
                     /locus_tag="VAH76_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1385704.1"
                     /translation="MNKVIFITGGSRGIGREIALKFAAKDKANIVIAAKTAEPHPKLE
                     GTIYSVAKEIEELGGQALPLMVDVRDEQQIQNAITKTIETFGRLDVLVNNASAINLTD
                     TLNTPMRRYDLMQSVNVRATFACSQAAIPYLLKSENPHILTLSPPLNMDKTWFAPHLA
                     YTISKYGMSMCTLGLAEEFKEAGIAVNSLWPKTTIATDAIRVHFPSELYMASRKPLIM
                     ADAAYWIINQPSKSTTGNFFIDEDVLKNSGVTDFSCYAMNPKAQLCPDLFL"
     gene            19399..19830
                     /gene="rnhA"
                     /locus_tag="VAH76_001058"
     CDS             19399..19830
                     /gene="rnhA"
                     /locus_tag="VAH76_001058"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEO1385705.1"
                     /translation="MKVEIYTDGACKGNPGPGGWGVLLRYNGREKTLHGGEAQTTNNR
                     MELMAAIKGLEALKRPCEVDLYTDSQYLQQGMKEWIKTWKRNGWRNSKKELVKNAELW
                     KSLDNLASIHNIHWHWVKGHSGHLENDLVDALANLGIEELS"
     gene            19835..20536
                     /gene="dnaQ"
                     /locus_tag="VAH76_001059"
     CDS             19835..20536
                     /gene="dnaQ"
                     /locus_tag="VAH76_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEO1385706.1"
                     /translation="MRQVILDTETTGIGPEQGHRVIEIGCIELIDRKLTGKHFHIYLN
                     PQREVDEGAFRVHGISTEFLQDKPLFQDIVTEFIQFVEGSELIIHNAPFDVGFLNSEL
                     KHVKWNKTLEDYCRILDTLILAREKHPGQRNSLDALCKRYEIDHFNRELHGALLDAEI
                     LAYVYLAMTGGQSSLFTEVESSMSHSKIKTQEIKSLSLKKPVIMKANEEELIQHQSFV
                     EFISKKSGINHWEEV"
     gene            complement(20743..21120)
                     /locus_tag="VAH76_001060"
     CDS             complement(20743..21120)
                     /locus_tag="VAH76_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385707.1"
                     /translation="MSRKLLIATTLVLSTSLFPLISNAEDTANPNEMTKDAWLNSMTP
                     LLPDLICKGFIQDPDLKKRFDEIKMTYEQCVTLIPESTKKCQDELYASMPDKINSETA
                     GTWGRSLGECIGKDFAEKHLIPK"
     gene            21412..21885
                     /locus_tag="VAH76_001061"
     CDS             21412..21885
                     /locus_tag="VAH76_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1385708.1"
                     /translation="MEKSNFRLNFLSQILLFTLGVFLFCNGYAAGNTFIFNPNTLTWK
                     AISANGKVIRTGRGSGGKHYCSDVRRSCKTPSGVFRVISKGGPGCRSSRYPLGKGGAP
                     MPYCMFFSKLYAIHGSPDVPNYNASHGCVRVKPHDARWLSQNFIKIGTKVIIKSY"
     gene            21993..23396
                     /locus_tag="VAH76_001062"
     CDS             21993..23396
                     /locus_tag="VAH76_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385709.1"
                     /translation="MIEVNIWLSTAFLFKKRIKHKFFGPLLASEDKGENVGHVNFTIE
                     IDERTKNSFDFIEKHGPELGAKKTLRAVPTQATGPSITNIESSHLKPTMVKSDVVSHS
                     FWPEGRPSKSETIKGKGVKPKFKTHEDDMIAEDSVTVMDIIHRKSALEEIGKEKMENL
                     DLLVEISDLEVNMEQRSLFMDDLKKLHLEKEELSKQQKQLTSSHQTQLKDLKGQLAKL
                     ELHLNKTNGQIKATERTLGYLNKLENPDLKTRAQSIELTEKLIKLKKELETTISSQHE
                     ISELMSKSELTYHEGMQKIEKNLQQVELAINHREENLRQLDIKINGRDENDLKELQEE
                     ARHRKEYISRKEQFVKSRDFTKGRHPDYSVTLPTAESGFTYYVDEVKILKAMQEERSQ
                     NYSFLFNNCASSAKRCLLAGIDEKLRAKLKEAGLDSKFFEISKVETCKSLRDWARTLE
                     SKLTELNFPPPAPTRGA"
     gene            complement(23461..23853)
                     /gene="erpA"
                     /locus_tag="VAH76_001063"
     CDS             complement(23461..23853)
                     /gene="erpA"
                     /locus_tag="VAH76_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEO1385710.1"
                     /translation="MASVDVSQNTSDIHFSVSAADKVAELIKEEDNSNLNLRVSITGG
                     GCSGFQYGFSFDEQINDDDTIIIQQCSDGKSSVKLLIDSMSYQYLHDAEIDYIKGIQG
                     EQFVIRNPNAKTTCGCGSSFSIGDEDDL"
     gene            complement(23988..25073)
                     /gene="mnmA"
                     /locus_tag="VAH76_001064"
     CDS             complement(23988..25073)
                     /gene="mnmA"
                     /locus_tag="VAH76_001064"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO1385711.1"
                     /translation="MKAKVIVGMSGGVDSSVAAWLLKEQGYQVEGLFMKNWEQDDHND
                     YCPAAKDLADAQAVCNQLRIPLHTVNFSKEYWDRVFAYFLSEYEKGRTPNPDVLCNKE
                     IKFNAFLNHALNLGADYIATGHYAKNTIEGSIGYLFKAKDREKDQTYFLHAVEPEALS
                     KTIFPIGDFTKPQIREFAKQLGLVTHAKKDSTGICFIGEKRFKTFLNEFILAKPGEIK
                     STGGKTLGQHDGLMFYTLGQRQGLGIGGLQNSTDEPWYVVDKDIASNTLYVAQGSQHP
                     MLYSQGLICGPIHWLADYENHLPLTCFAKTRYRQTDQACMISPPDNNLHYVMFSSPQR
                     AITPGQFIVFYEKNQCLGGATIEQIIR"
BASE COUNT         7944 a         4394 c         5121 g         7682 t
ORIGIN      
        1 catgccctta atcattttcc ccaagcaagg tatagagtgt tatgttaaga gggatgatga
       61 attaggttgc ggcataagtg gtacaaagat aaggaaatac agcagtttaa tcccgtttct
      121 aaaaatcaag ggaattcgcc atctcattat tattgcgggt gctcaatcta ataatttact
      181 ggctgctctg caggtggcca gagaatgtca attgaaagtg accgcttttc tagtaaaacc
      241 caaacattta aaaatccagg gtaattttaa attaagcctt ctttttcttc atgagaatga
      301 aattatctgg ataaatcgag aagaatggta tcgagtcaat gaattcgctg agcagtatct
      361 tgaggggctc agagaaactg gttatatttt atctgaaggt gccagtgtta aggagtccat
      421 gaagggggca atgtctctag ccaatgatat caaggaaaat gaaaagattt tgggatttgc
      481 ttttgatcat atctttgttg atgctggtac aggtttttca gcaatcgcgc taattaaagg
      541 ttttcatgaa ttacagcata aaggatttat tcatgtgtta ttgttagcag atagtgagga
      601 ggtttttaag aaaaaactta tgcattgggc aggagtaaac cctgataatt acgcttgttt
      661 ttacccaacc actgcgaaat cgtttggatc tgtaaatcaa acaattaaac aagaaattaa
      721 acgattggct tatgaagaag gcattttggc tgatccgatt tactctgcaa aattatttta
      781 tgaggccaga aggtacatag aaaaatacga attaaaaggg aagatattga ttgtacattc
      841 tggtggtata ttaaccatgc ctggctttgc tttttgaata ggcttgcttc ttggcaaaga
      901 cgtaatttct gaattatcac gttttcctga ctgcaaaatt aagttcatca aggatattca
      961 gtatatttca gtacataatg cgattttttt cctgaatatc tctgtctcga ttatctacta
     1021 tacttatcaa ttataaaaat aaaaaccgtt ctttttaaga aaagttatct tatatgggag
     1081 acgagtatca tgaaagcaag aagggatcaa caactctcta aattgcgaat gagatttttt
     1141 tcagcgctca atcacacatc tgaaattgac ttacacgtgc tttttaatga tttgaaatca
     1201 attctcacac tggatagtat taagcatctc aaagaaggct ctgtagcata tgcaatcatt
     1261 caagaactct tgaaacagga tgatgctcaa aataagattc aatctttttt acatggagca
     1321 atcaagaatg ttattcaccc gggtgttatc aaagggctta cccctgatga aattaactgg
     1381 aatgttgcca aagcataccc gaaatattat gagcatgagg aatttcctga tgtgactttt
     1441 ggcggtttta aagtacgtga tagtaatgaa tttaaattta agactaatat acaaacttca
     1501 atatggttta gtatcaagcc cgatcttttc atgccatcta aacagcagga ggctttaaaa
     1561 agaagaaggg aacaataccc tggttgcgaa attagattaa tttattccag ttcattgcta
     1621 aatgcagaag caaaccgcca gatgaaggca tttgccagga aacaaaatat ttcattaatt
     1681 gatatcgata gtgttaaaac caattcacca ttatatccat tactaaaatc tgaacttgca
     1741 caccttggaa aaggaggaaa tcctgctgct gcctccgatc tgtgtcgctg gatccctgag
     1801 gtattcaatg aagggtttta tgtggatatt gatctccctg ttgatagttc aaaaatagta
     1861 gaaggacatc aaattacagg aggagttcca atcatgctca atatgggctc cattatcagt
     1921 gaacccatag ctccacatca tagaaggcaa gaagctgttt gtatgaatac ggacatcatt
     1981 gcttattcca atgataaaag aactcaaaaa atgatggata cggtcgcccg ccatttgaaa
     2041 aatatatacg atgatccata tacagcatta aaagacacac ctcttgcgca aacggctttt
     2101 tttaataagt gccaagagga aaggaaaagt atatttgatt taagaaaagg attacaagat
     2161 gcatttcgct ccgattcatt attgcagtta tacgactttt taggggcaga caaatttaag
     2221 gaagtattta aattaaagga agctcaaagc aaatacatta atgagcatat aagtgaattt
     2281 agtgagaagg atctgttgtt aaatttgatt tctgataaac cttccgagat taatcagcat
     2341 acacttgatt ttgtcaaggc taaagcaatg tacatagata ttgccaaaga gcactattct
     2401 gcattctaca aaccattggt tgaggagatt tcagggccag gtgtcattta taatgcttta
     2461 ggaggagcgg gtagcttcac taccactcat aggcggttaa caggacctat gttgccaaca
     2521 actccaccac gtgttttaca ggtattttgt gatgcgcatg acaagggtcc ttttgtttca
     2581 gataatattg cgcgttggca aaccaatgtc cgagatctgg gcgtgttgaa tcgagagggt
     2641 ttgagttggt tgccttcagt agggtagctt aattccaaca cacaaatttt gaatattgtc
     2701 tcaagcattt gcaatgtgaa taatcaccaa tagataaaag actttattgg tgattagtgt
     2761 tttttgctgg tttcctcctt ttttagcgct atccccttga aatttaatag atcaacccta
     2821 tatgagaagt taattattcg aacacaacta ttaacgggga gattgtttaa tggttagcaa
     2881 gcagcaaact atgggatttc aaacagaggt aaagcaaatg cttcatttgg ttgtgcattc
     2941 gctttatagc aacaaagaga tttttcttag agaattaatt tctaatgctt ctgatgcctt
     3001 ggacaagtta cgatttttag ctttatcaaa cggttcattg tttgaaaatg attctgattt
     3061 gaaaattagc atccagatta atgaaaagct tcaaaccatt accatcagcg ataatgggat
     3121 tggtctgagt tgggaagagg cagtagaaaa tttggggaca attgcaaaat ccggaacaaa
     3181 agaatttatt agccaattaa ctggtgaaca ggcaaaagat tctcaactaa taggacaatt
     3241 tggagtgggt ttttattccg cgtttatcgt tgctgataaa gtaacagtta aaagtcgccg
     3301 cgctggatta caacctgagg atggaattgt atgggagtct aagggtgatg gtgaatttac
     3361 tataggatat gaaaaaaaat cgacccgcgg tacagaaatt accttgcact taaaacctga
     3421 aaacgacgag tttttaagtg attggcgtat tcgcggtatt attagtaaat actctgatca
     3481 tatttgttgg cctattgtga tgaaaaagct ttcagaggaa ggcaaggaat caaaagaatt
     3541 tgaaacagtg aacaaggcga cggcactttg gacgctacag aaatctgaaa ttagcgaaga
     3601 agactataag caactgtata aacatatttc tcatgattac atggatccat taacctggtc
     3661 tcataaccat gttgaaggaa aacacgagta tattactttg ctttacatac cagcccatgc
     3721 cccatttgat ttatggcagc atgaagccaa acatggacta aagctttatg taaagcgtgt
     3781 atttattatg gatgaggcga cacaattttt accccgatat ttacgatttg taaaagggat
     3841 tgtggatgcc agtgatttac cgcttaatat ttctcgagaa atattacagg ataacaaaca
     3901 ggtagagtca attcgagctg catgcaccaa acgtgttttg tctatgcttg aaaaaatggc
     3961 taccaatgat aaagaaacct atcagaagtt ttggaatgaa tttgggcttg tgctaaaaga
     4021 aggacctatt gaggatttcg ctaataagga agcgattgcc aagttgcttc gtttttctac
     4081 tacagcaagc ggtagtgaga aacaagaagt atcgctagag gaatatatta gtcgcatgaa
     4141 agaagggcag gataaaattt attatattac cgcatcgagc tacaatgctg ctaaaaatag
     4201 tcctcatttg gaaatattca ggaaaaaggg aattgaggta ttattgctca gtgataaagt
     4261 cgatgaatgg cttgttggct atatgaatga gtttgcaggc aaaaaactgc aatcaatttc
     4321 aaaaggcaag attgaattgg gggatgatga aacttccgaa caaattaaag aacaggaaaa
     4381 aaccctggag ccattgatta agcatattaa atcagttcta aatgagcgag ttaaagatgt
     4441 attgctcact aatcgtctta ctgattcccc tgcatgtgtt gttgctgatg agcaagacat
     4501 gggattggaa atgcaaagaa ttttacaagc tgcgggtcaa caggttccag tgagcaagcc
     4561 tatttttgaa attaatccgg atcatgctct gattaagcgg ttgcatgata ttcaggatga
     4621 taaccaattc gaattatggg ttactatgct gtttgaacaa gctgtgttgg cagaaggagg
     4681 gcaattggat aatcccgctg attttgtgaa tcgggttaac agactcctgg tttcctccta
     4741 gaattctata gggttggcaa tcaatcccgg attagaacaa gtcattatcc gggatgagct
     4801 gaacataaaa taaaaaggct caaaaatgag cctttttatt tttaaatttc ccttactctt
     4861 cagttaagcg aaaatatttg gtaccgaaat atctttgtaa tttctttctg atcgtaccgc
     4921 gactaatacc caacatcttg gctgcttgta attgatttcc acgacgtttt tccattacag
     4981 cttgtaatag aggtggttca acttctgaca ggatcatatc atagagatca ttaattgatt
     5041 tggatttatt ttcagcaaga aagcgggtaa ccaaactgta aactaaatcc tgtaaacctt
     5101 gttcttgttt agtggattga gttgtagctt gtgtatcaat gacattcatc ttaacttcct
     5161 tattatttaa ttaaatcata cagtccaatt gcttacacat gtaaatgaaa gcaaaattaa
     5221 ttgtacatga aggctaaatg ataatctaga ttttcatatc aaaactatgc atttttttca
     5281 ggcaattctg ccactacaag atcaaagaga gaacagtcag cctggctgga agacagagat
     5341 tcctttaact ttgccagttg attaatcatc gacttgggtt ggttaggatc attatgaaaa
     5401 ttagaaatat atctagataa ttcaattgga ttgcagtcgt attgcaaaaa ttcaggtacc
     5461 atcattttat tggctaacag attacataag cccaaaaatt taactttaat aaaatacatg
     5521 gcagcaacat aagtaagaaa ggacgatttg taaataatgc acattggttt ttccaataag
     5581 gcacattcta atgaggcagt tccagaagct actatgacaa agtcagcagc agacatgcat
     5641 tctattgcct gtccctggat aaaagtaaca gtaagattct ggttgctgaa ataagctttt
     5701 actttatcag gattgatagt tccagcgata gggactacaa atcgaagttt ggggctgtct
     5761 agggtcagca attttgctgt attcaccaat atcggtatat ggcgttcaat ttcagaatgg
     5821 cgactcccag gcagtaaggc aattattggc tcatttaaag gtaatcccaa agaagttctg
     5881 cttgagtgtt tatccttggc agcagctatc ttttttacta aaggatgtcc tacaaaactg
     5941 acaggaacac ctgcattttc atagatagtc ttttcaaatg ggaagatgac agccatttta
     6001 tcgatgcaat ctttaataag atggatacgt ttgcctttcc atgcccatat ttgagggctg
     6061 atgtaataaa tgattttgag ccccagtttt ttctttgcgt acttggccaa tctcaaattg
     6121 aaagcagggt aatctaccaa aatcagcaaa tcaggttttt gtgtgctcag atgctgtttg
     6181 atatcctgaa aggctttacg gaatattttt aggaaaggaa ttatttcagt caaaccagtt
     6241 acagcataac gcgttaaatc tgaaattaaa tgaaccccgg cggctcttaa gtgttttccg
     6301 ccaatgccac taatttccag gtccggataa actgctttta attgctttac cagctcggca
     6361 gcatggtggt caccagattc ttcacctgcc actatgacaa cacgactagc attcggcata
     6421 gcgttctaca agattattat taattaaagc ggtgatttgt tcggctgttt taagggcatc
     6481 tcttccttcc tcacctgtaa caagcggtac gctatcattt tctatgcaat tcaagaaagc
     6541 tttgatttcc tccagtaatg catctccttt ttcaaattcc ctttgatctc tggtgatatc
     6601 aggtattcca gggaacattt ctcccgtacc ttttttaaat acagcaagct gtttgttttg
     6661 ataatcaatg gaaatataag aattctcttg aaagattctt gtttttcgtt ccgtcttgaa
     6721 actgattctg ctggctgtca catttgctac acagtggtta gcaaagctta tgcgtgcatt
     6781 agctatatca attgcttttg ttaataccgg tgctccttga gccgtaatgg atttaatagg
     6841 actctgaact atattttgaa taatgtctat gtcgtgaatc atcaaatcaa gtatcacgtt
     6901 tacatcagtt ccacgggggt taaaaggtgc gagtctctgc gattctataa ataatggtaa
     6961 ttccagatat tcatccaagg caaggcgagc agaattgaag cgttctaaat ggcctacctg
     7021 taacttcacc tggtgttgtt ttgccagtcg aatcagctcc tcagcctggg atactgtttc
     7081 agtaattggt ttttcaatca aaacatgtat cccatgttgt aaacagaatt ttgcaatttc
     7141 aaaatgcttg ttggtagtcg ctgcgatact gacagcgtct actttgccca acaggtcatg
     7201 gtaatcgatg tatgccggaa catttaactc actggaaacc acttcgcaga ctgtagaatt
     7261 tacatcacaa actgcaacta actctgcatt aggaattagc tgatattttt gggcatgaaa
     7321 cctgccaaga taaccaacac caataactgc acaacgaatt ttattcatct catcatcaat
     7381 tgataattat ttgtacttat gatcgcattt ccttagtgat aaatcaattg tatttcctat
     7441 gttgtcatgc caggtacata gtaattgagg catttaaaac tccaatcaaa cttctaagat
     7501 tggatcaaat caaagaagtc gattcttgtc ttgcacttgc attcaatagc tattatctta
     7561 cgcctaattt ttcgcaggga acagttaaat gaatactgag ctaaaaatat tgatggctgg
     7621 ggtcgatgaa gtggggagag gaccacttgc gggtgccgtt gtaaccgcag ccgttatttt
     7681 aaaaaaacca atagatgggc ttaccgactc aaaaaaatta agtccaaaac aaagaaattt
     7741 attagcaatt agaataaagg aagaagcgtt agcttttgcc tatgggcgag cagaagttga
     7801 ggaaatagat caattaaata ttcatcatgc tactttatta gctatgcgaa gggcagttga
     7861 agctttaccc attcagccag ataatgtcgt tgttgatgga gcttttactc ctcaattgaa
     7921 cataccatgc aaggctattg tccagggaga tagcttggta cctgaaatta gtgcggcatc
     7981 tattctcgcc aaggtattgc gtgatgaaga aatggtagct cttgataaaa tatatccagg
     8041 atatggtttt gcagaacata aaggttatgc aacccctgtt cataaagaag cgctaatgcg
     8101 tttgggtcct tgtagaattc atagaagaag ttactcccct gttgcggatt tgatttcaaa
     8161 gtaaaactct gagtggtatt aatttggaaa ataccgttca aataaaaata tataagaaat
     8221 agcttttaat atgaaaatca gagcaaaaaa aattaaatca attcaaacta ttaaataata
     8281 ttcggtgtga gcttatcgac attaaaattc caaagcttgc caggaaagtt accatagctg
     8341 ttccaccata actgacgagc ggaagaggta tacctacaac cggaataatc cccatgacca
     8401 taccaatatt gacaaatgca gaaaggaaaa aggtcatggc cagactggct gaaagcagac
     8461 gcgtgtaagt ggtttgtgca ttattggcaa tatttaaact gcgtagcgaa attaagacta
     8521 tcagtgcaac aatagcaaat ccgccggcaa atccaaattc ttcgccgctt accgcaaaaa
     8581 taaaatcagt agcatgttct ggcaagaaat tgaggtgtga ttggctacct tttaaccatc
     8641 ctttacccat tagtccgcct gagccaatgg caattttgga ttgaataatg tggtagccag
     8701 caccaagtgg atcttgttca ggatcaatca gggtatatac tctttgtttc tggtagtcat
     8761 gcatcagatt ccagacaaca ggaattgctg agcacatcag caatgcaatt aataatatga
     8821 ttttaaaacg tattcctgcc aaaaatacaa cacataaacc ggcaacagta accataattg
     8881 cagtacctag atcaggttgt tttgcaataa gaagagccgg aataaaaata atcagggaag
     8941 caataccaat tgagcgtatg ctgcttggat gagattgtcg gtcgaaaaac caggcggcca
     9001 tcattggtac agccagtttc ataatctcgg aaggttgaaa acgaaataac cccagttcta
     9061 accagcgctg agccccttta ccaattttac ccattagcat gactgcgatt aacaagctta
     9121 acccaacccc gtaaatccat ggagtccaga ttttatattt atgtggcggg ataaatccca
     9181 gtacaaacat gattagaaaa gcgaagagca gcctcatgga ttggcgcatg atcatgccca
     9241 tattggcatt tgaagcgcta taaagtatca agagtccgaa ggcaataaga gtaagtatta
     9301 atccaagtaa tggaaaatcc agatgaaggg actttgctgt aaaacgatat acgggcttag
     9361 tcgtttgtct gctcatattg ttttcttaat aggataaagt tgataataag tgtcaagtac
     9421 cttacgtgct acagtggaag caatagtgtc attttcaact accaccgcaa tggcaatttc
     9481 cggtttttct actggagtaa atgcaataaa taaagaatta tccctcaaat gctcaggtat
     9541 gtcttcgtat tttgcctttt catattgtct tccgctgtat acttgtgctg tacctgtttt
     9601 tcctgctacc gggtaaggag gatttctgcc aaaacggtaa cctgttcctt cattgctggt
     9661 tagtacgcta tgcatcgcat caatcacaac atcccagttt gcttcatctc tcagggaaac
     9721 cgggtattct tcaaaaggct tgtattcctc cgtttcattg gtatcgctgt ttactgtttt
     9781 tatcagtagg tgaggtctga atcgttgtcc atgctggctt agggaagctg tggcattagc
     9841 catttgcaaa ggggttgcca gcataaatcc ttgtcctatt gcagaaatga gagtatctcc
     9901 tggataccag gatactcctt ttgtttgtct tttccagcga gcgctaggta ttactccatt
     9961 tgcttcttca tgaaggtcaa cgtgtgttag ttgtcccaag ccaaatttta caagcatgtc
    10021 ctcaatgtta gagattccca ttttattacc tagttggtaa aaaaaagtgt cacaagagac
    10081 ggtaatggct cgtttgaaat taatgacccc gtggcctgtt tttttccaat cacggtaaac
    10141 atggctggaa gtagggagtt tgtacttacc ggggtcatat atttcagtgg tcgtggtaat
    10201 gaatccttta tccaaaccag ccagtccaac aaatggtttg attgttgatg caggggggta
    10261 aacacctcga actgctcgat taaaaagagg gcgctggagg gcattggata acactttata
    10321 atccgcttta ctgactccac tgacgaaaat attgggatca aaacttggag aactcaccat
    10381 cgcaagtact tcgccattcc gagagtttat gactactacc gcacctcgtt tatccttcaa
    10441 tgcattataa gctgcttcct gaagtctggc gtctatgctt aaatacagtt tcgctccaga
    10501 atgaggattg actttattta ttattcttaa agtgcggcca ctaacatcag tttctaccat
    10561 ttgataaccc actttaccat ggagaatatc ttcatagtat ttttcaatac cggccttgcc
    10621 aataaagttg gtagcacgat aattagtgga gtccacttgt tttagttcct gaatattaat
    10681 gcgaccgaca taccccaagg catgggcagt aatctctcca agagggtaat gacgcatcaa
    10741 tctcgcttta atgcttactc ccgggaaatg atactgattg ctggcaaagg tagctacttc
    10801 ttcctggctt aattttaatt tgataggaat gggaacaaaa gaacgatttt gtaatctggt
    10861 cttattgaag ctatcaatat cttcgtctgt aattgaagga attaactctc gaagtttcgt
    10921 caaggtctgt ttcatgtctt taacacgttc gggagtgatt tccagcacat acactggtat
    10981 gttttcagcg agtaatactc catttctatc caaaataacc cctcttggag gagcgatggg
    11041 tatcacgctc atttgatttt ttaaagataa ggtttgatat cgtttaaatt cggatatttg
    11101 caggaaggcc aatcttaaaa tcagtattaa agataaaata atcagaatcg caattaatag
    11161 gttaagtcta aatcgttgat gttgggattc agcacgatag tttttaaatg attgattccg
    11221 atacatcgca gaaaatgttg tatgacaaaa caaaagtagg aatagtacct gaaattacag
    11281 atttttacca attataaaat atttataaag cttgtggttt ttcatttttt gagattagag
    11341 tcaaattttt ctatgtaata tagaaggagt gttgcaaaag actatgcaca ccctatttat
    11401 gataaggatg gttgttaatg atagaccatg ctctatagag ggattccagc aaaataatgc
    11461 gaaccagagg atgagggagg gttaatttgg ataatgacca gcgttcatcg caaagagaca
    11521 gtatttcatt ggacaatcct tctgggccgc caattataaa acaaaggtga cttgaaattt
    11581 gttgtaattg cgtcaactta agtgctaact cctcgctgct aaaggatttt cccagcatgt
    11641 ccagggcgat aaggcgagca ttggcaggaa gggcatcttt aatcaaagca ctttcttttt
    11701 ccagtattct tgctaaatcc gaggatttat tacgacgcag caaagggatt tcaatgagtt
    11761 ttatctgaat gccatcatga aacctctttg cgtattcatt aactcctgag ttaacccagt
    11821 cgggcatttt attgccaaga gtgataatgg taatttttag catagataat atcaggagtt
    11881 acctgggtgt tcttcccata atccttcaag gttataaaaa tgtcggaact ctggttgcat
    11941 gacatgcaat atgatgtctc caaaatcaat taaagcccaa tcaccatgtt ctactccagt
    12001 agaactcaag caaggcaaac cagcaacctt catatcttcc agaacttttt gagctatcga
    12061 tttgacatgt ctggatgaac ggcctgatgt gatgaccata aaatcagtga ttgttgtttg
    12121 tttatgcaca tcaatgattt taatatctat cgcctgaatg tcttcaagag attttaacag
    12181 tttttcgagc gtaggatttt tttcaagcat aactaagata tattccgtca aaagggttga
    12241 ataatatcaa caattgattt gtataaaaag agttttagtt ttccaggcct ggaaaactaa
    12301 aacgtaaaaa tctaattatt gtttttcgca tatctgaata tgggtcaata aaaatttaat
    12361 cacagcaaac aataccaggc agcccaatgt agcaatgaac aatatgctga gttgttgggt
    12421 gaatgctgta gcatatagtg tttttaaaac gattatattt gtctctccag taggaatggc
    12481 tgtcagatca gctaatttcc ctgaaaggaa tcctcctatt cctaatgaaa cgaaaaagat
    12541 acccatcatg gtgcttacct tgtttttgtc agcaagtaca gtgattgctg acaatcctac
    12601 tggggaaagc agtaattcag ccaaagaaat catcagatag gcagggatga taagaagagg
    12661 tgaaatcaaa atagatttat caactacagt acttacaaag gcaataattg agtaggcgag
    12721 agtcatgaat accatagcaa gtacaaactt tttcccggta cttaagcctc tttcaatcat
    12781 tgtaagatta tggtgtcttt tggcaagaaa ataaccaatt attaacatgc caatactttg
    12841 tactgtgatg tagtatggtg gcgggaaatc catacctaaa aagcttggct cgactactcg
    12901 cgcaataaat aatgttaaag acatgaacat ctgaaaataa aatgcccaga aaataacaga
    12961 gatgatgcat aataacccga taactaatgt ttgcctggat tgatttgcac tttcacgatt
    13021 tactgaatat aaaatgtaac tggctgaaaa aagaactaca agtccaaaca tgatattcgc
    13081 cagctgaggt gaacttaaaa tataaaatga caatgaccac agaaaaacca ataacacaaa
    13141 agctgaaatg attttacctg gttggaaaac aaaaggatta taatccttaa ttttgtactg
    13201 atgtattcca aataaaaaca ctacaaatgc gatgattagc ccgattgatg cactggtgaa
    13261 ggaggcagac cagccaaaat actcactaaa cttgcttggc aatgttgttc cgaggattat
    13321 tcctgtcgtg atgcccatat aaaaaatagt aaaaccactt tctctttttg ttgagccaac
    13381 tggatattca ttacctagta atgaagaaat gttaggtttt aataaacctg ttcctacagc
    13441 tactcctgca agtgaagagg ttaatgccag agaattatca atcaatgaca acatgcaata
    13501 acttaaaaac agaacaatag cacccagcaa tatagcgcgt ttttggccta ttaatttatc
    13561 agcaatccag cctcctacta atggtgagag gtaggtgagt gctgtgaacg agcctactaa
    13621 agaatagatt tctttgtccg gccatttaaa gtgtaaggcc aaataaagtg ctaataagga
    13681 ttgaactgcg taaaagccat aacgttccca catttcggtt gtaaagtaga tgcggagaga
    13741 ttttgggtgt ttttccatat tatttaagta actacgatta aattaattta ttaaataaaa
    13801 attatatcat ataaaaaaca gtatgaatat tgattggtca ttttatctgg tttttctcaa
    13861 ctcattagat tatctcattc ccggaggtag ttgactggtg tatgatgcta aaaaattttt
    13921 taatcgctat ttgataggaa ataataactg aagttttcat agcaataagg agtattcttg
    13981 atgagcaagg acaagaataa acgtgttatt tctgccccta gaggtaccga aattcaagct
    14041 aaaaattggt taactgaggc ggcattaagg atgatttgca ataatttgga tcccaatgtt
    14101 gcagaggatc ccgattcttt gattgtttat ggtggattgg gcaaggcagc cagaaactgg
    14161 gaatgttttg atgaaattgt tcatgtattg acattattaa ataatgatca gactttactc
    14221 attcagtccg gaaaacctgt cggtgtattt actacccatg aagaggctcc cagaatacta
    14281 attgccaatt ctaatttggt accgcgctgg gctacttggg agcattttaa cgaactggat
    14341 aagaaaggcc tgatgatgta tggccagatg accgccggca gctggattta tattggttct
    14401 caaggcattg tgcaaggaac ttatgagaca tttgttgcgg cagctaaaaa acattatcag
    14461 ggtgatttgt ccgggcgttg gattttaaca gcgggtttag ggggaatggg tggagctcaa
    14521 cctttagctg gaactatggc tggtgccagc gttctggctg tagaatgtga caggcaaagg
    14581 attgagaaac gtttacagac gaaatatctg gatcggtaca ctgataattt aagtgaagca
    14641 ttagactgga ttaatgaatc ctgtcgctgt aaaaagccag tttcagtagc tgttttggga
    14701 aatgctgccg aaatatttcc tcagctggtt aagttgggag ttcacccttc cctggttacc
    14761 gatcaaacca gtgctcatga tccgctcaat ggctatttgc cattaggatg gacattggaa
    14821 caagctgttg aaatgagaaa aaaatcacca gaagaggttg ttgatgcagc caagaaatcg
    14881 atggctgttc aagtgcatgc aatgcttgag tttcataaca gaggcattcc tgtatttgat
    14941 tatggcaata acattagaca aatggcattt gaagcgggag aaaaaaacgc tttttccttt
    15001 gagggatttg taccggctta tatcaggcca ttattttgcg aaggaatcgg accatttcga
    15061 tgggttgcat tgtcaggtga tccagaagat atttatgcaa cagatgagcg agtaaagcag
    15121 ctaattcctg atgatcctca cttacatcat tggttagata tggccaggga aaaaatttca
    15181 tttcagggat taccagcaag gatatgttgg gtagggttaa aggatagagc gcgccttgcc
    15241 cttgctttca atgaaatggt taaaaataaa caagttaaag cgcctattgt gattggccgt
    15301 gaccatttgg attctggttc tgtggccagc cccaaccgcg aaacagaggg tatgctggat
    15361 ggcagtgatg ctgtttctga ttggcctttg cttaatgcgc tgttaaattg tgccagtggg
    15421 gctacttggg taagcattca tcatggggga ggggttggta tgggcttttc tcaacatgct
    15481 ggcgttgtga tcgttgctga tggaacagaa aaagcggcta aacgattggc tcgcgtgcta
    15541 cacaatgatc ctgcgacagg ggtaatgagg catgctgatg caggttatca aattgccaag
    15601 caatgtgcca aagagaattc gctttggcta cccatggaat cttaaaagga gcaggtttat
    15661 gtcagaacat tttatattgc agcctggcca gttatcgcta ctttctatta aacaaatttt
    15721 agatgaggaa ctatcttgcg ttcttgctga gaacgccttt gaattaatta gagcatctca
    15781 ccaaacggta aaaaaagtaa tcgatgaaaa gaaaacagta tatggaatca atacaggttt
    15841 tggttctctg gcaaaccaga ccatttcttc agattgtttg aaagagttgc agcgtaatat
    15901 tgtactttct catgcttgtg gaacaggtaa attattgccg gatgacgtgg ttgccttgat
    15961 tttgcttttg aaaattaata atttgtctca agggtattcc ggagttaggc tgcaattaat
    16021 caatgcattg attgctttgt ttaatcataa agtatatcca tgcattcctt ctaagggttc
    16081 ggtaggtgcg tccggagatt tagtcccgct tgcgcatttg tctttacctt tgttaggaga
    16141 aggcgaagta aggcatcaag gtcaggtgat cagtgctgag gaagggctaa aactggctgg
    16201 attgaaaccg cttgaattgg aagctaaaga aggattagca ctgcttaatg gcttacaggt
    16261 atctaccgct ttagctttgt cagctctgtt tattagtgag acattgtttg aaacagcgat
    16321 tatttctggc agtttgtcag tagatgctgc cagtgggagt gatgtgccct ttgatgacag
    16381 aatacatcaa attcgaggac atcaagctca aatttctgct gccaggatgt acagaaattt
    16441 attggcaggc agtcaaattc gagagtcaca caggtattgt aatcgtgttc aggatcctta
    16501 ttctttaaga tgtcagccac aaatcatggg ggcagttctt catcagatgg agtttgttgg
    16561 acaaactctt caagtagagg ccaatgcaat ttccgataat cctctcgttt ttgcagagca
    16621 gggggatatt ttgtctgggg ggaattttca tggtgaaatc atcgcaatgg ctgctgacaa
    16681 tttagcattg gcattatccg agataggagg tagtgcagaa cggcgaatcg ctttattaat
    16741 tgataaaaat tttagtggat tgcctgcttt cctggttaga gaaagcggct taaattcagg
    16801 ttttatgatt gcccatgtta ctgctgcctc ctgtgccagt gataacaaag cgcttgctca
    16861 tccgcactcc gtcgatagtt tgcctacctc ggcaaatcag gaagatcatg tttctatggc
    16921 aactagcgca gcacgtcgac tgcatgaaat gatcgataat acttcaacaa ttcttgctat
    16981 tgagttattg gcagcttgtc aaggactgga atttcacaaa cctctaaaga catcaccgca
    17041 attggataaa atttatcaaa gcgtaagaag cgtggttaaa gagtatgata aagatcgcta
    17101 ttttgcgcct gatatagaaa aaattaaaaa gaagatttta gataaggaat tctcattact
    17161 cacactcacg aattaatagc aggaaaaata caatggaatt gatggcagaa tttctagctt
    17221 tgcatcaaca acacaaaaac aatccttatc cttctttgtc tgaaagaaaa aaattgctga
    17281 ttgcaataaa aaaaatcttg cagacagaag cttacacttt ggcagaagca ataaataagg
    17341 actttactca tcgccctgtt gaggaaacct tatttttgga aatttttcca acgataaaag
    17401 ccattaattt ttgtttaaaa aatatgaaga aatggatgag aaagagacga aggaatgtgt
    17461 cttggttgtt tattcctgca aaagcttatg ttattcccca acctcttgga gttgtaggca
    17521 tcctggttcc ctggaattat cctgtctatc tggctttagt acctgctatt tatgccttgg
    17581 catcgggaaa cagggtaatg ataaaaatgt ccgagttatc tccacatatt ggcgatactt
    17641 tacaaaaatt ggtacatgca agtggaataa accattctat cagcatcatt aacggcgata
    17701 ttgagctatc aaaacacttt gcttctctgc cttttggcca tcttatgttc actggttcaa
    17761 ctaacgtagg aaaactggtg atgaaggctg ccagcgataa cctgacccca gtgacactgg
    17821 agctaggtgg taaatctccg gcaataatat ctcccaccat gaatccagct tattttaagc
    17881 gcctgtttat ggggaaatta ttgaatgccg ctcaaacttg tattgcacct gattatgtat
    17941 tgattcctaa aggctgggag gagagagttg aaacagagtt taagaagttt attaatacct
    18001 tttatccaga tttaatgagt aatgagcagt attccagtat catatcagag agccataaaa
    18061 agcgattact tgatctggtc gaagatgcgc gaagcaaagg ggcccgcgtt gttgagtttg
    18121 gtcatttaat tccgaatagc tcgaagctac ctgtgttttt attatttgat ataacaaatg
    18181 atatgcttgt tatgaaggag gaaatttttg gccctatact gcctgtattg acttataatt
    18241 cgataattga tgccgtagat ttcatcaatt cttttcccaa tccgcttgcg ctatattatt
    18301 ttggagaaga taagaatgaa ataaagatca tacaaacaac aacattatct ggtgctttga
    18361 caattaatga aaccttaatg catattgcaa ttgatgattt gccatttggc ggtgttggcc
    18421 atagtggtat ggggcattat catggaaaag aaggatttga taccttttca aaattaaaac
    18481 cggtattagt gcagcgattt atctctacag tgagctggtt atatccacct tatggtgctt
    18541 ttatgcgtat gtttttggct tgggttggtg gtatcaagta gaaggagaaa tcatgaataa
    18601 agtcattttt attacgggtg gcagcagggg aattggtaga gaaatcgctt taaagtttgc
    18661 agcaaaagat aaagctaata tcgttattgc agcgaaaaca gcagaacctc atcctaaatt
    18721 ggaaggaact atttattcag ttgcaaaaga aattgaagag ttaggcggac aggccctgcc
    18781 tttaatggtt gatgtcagag atgaacaaca gatccaaaat gctatcacta aaaccataga
    18841 aacttttgga agattggatg tattagtcaa taacgcgagc gcaatcaatt tgactgatac
    18901 attaaatact ccaatgaggc gttatgatct catgcagagt gttaatgtca gggcaacgtt
    18961 tgcatgttct caagcagcca ttccatatct tttaaaaagc gagaatccgc atattctgac
    19021 cttgtcccca cctttaaata tggataaaac ctggtttgca ccacatctgg cttataccat
    19081 cagtaaatac ggtatgagca tgtgcacatt aggcctcgct gaggaattta aagaggcggg
    19141 aattgctgtt aattctttat ggccgaaaac tactatagct actgatgcca taagagttca
    19201 ttttcccagt gagctgtata tggcaagcag aaagccactc attatggctg atgctgctta
    19261 ttggattatc aaccaaccat caaaatcaac cactggaaat ttttttattg atgaggacgt
    19321 attaaaaaat agtggtgtca ctgatttttc ttgctatgct atgaatccta aagctcaact
    19381 ttgtcctgat ttatttttat gaaggtagaa atctatactg atggtgcatg taagggaaat
    19441 ccagggcctg gcggctgggg cgtattgctc cgatataatg gtagagaaaa aactcttcat
    19501 ggaggagagg cgcaaactac caataatcgc atggaattaa tggcagcaat taagggcctg
    19561 gaagcattaa aacgtccttg tgaggtggat ttgtataccg actcccaata tttgcagcaa
    19621 ggaatgaagg aatggatcaa aacatggaaa agaaatggtt ggcgtaactc caagaaagag
    19681 ttggttaaaa atgcggaatt atggaaatca ttagataatc tggcttctat tcataatatt
    19741 cattggcatt gggttaaagg gcactcaggt catttggaaa atgatttggt tgatgctttg
    19801 gcaaatttgg gtattgagga gttatcatag agttatgaga caagttatat tagataccga
    19861 aacgactggt attggacctg aacagggaca tagagtaatt gaaataggtt gtattgagct
    19921 tatcgataga aaattaaccg gcaagcactt ccacatatac cttaatccac agcgtgaagt
    19981 ggatgaaggg gcatttagag ttcacggtat aagtactgaa tttttgcaag ataaaccttt
    20041 attccaggat attgtgactg aatttataca atttgtggaa ggttcagaat taattattca
    20101 caatgctcca tttgatgttg gatttctgaa ttctgaatta aaacatgtga aatggaataa
    20161 gacattagaa gattattgcc gaattcttga tactttgatt ttagccagag aaaaacatcc
    20221 tggacagcgt aatagtttgg atgctttgtg taaacgttat gaaatagatc attttaatcg
    20281 tgaactacat ggtgctttgc ttgatgccga aatattagct tatgtgtatt tggcaatgac
    20341 aggtggccaa agcagtttat ttactgaggt tgaatccagt atgagccatt caaaaataaa
    20401 aactcaagag ataaagtcat taagtttaaa aaaaccagta ataatgaagg ctaatgaaga
    20461 ggaattaatt caacatcaaa gtttcgttga atttatatct aaaaaatccg gtatcaatca
    20521 ttgggaagag gtatagctct catgattata taaattataa ataattattc tgttacgctt
    20581 attattcggt ttcgcgtctg ccaatactaa gccttataca ataacaaaat tcttcagaca
    20641 tcttattcta ggtcgctatc ctcaaaacaa gcaacccaag tgattggtta taggcagagg
    20701 cgaagcctaa tgattatctc aatcaagcaa tttttttatt atctatttgg gaattagatg
    20761 tttttcagca aaatcttttc ctatacattc tcctaatgat cttccccatg tgccagctgt
    20821 ttcactattg attttatctg gcatgctggc atacagttca tcttggcatt ttttggtgct
    20881 ttcaggaatt aaagtcacgc attgttcata agtcatttta atttcatcaa atcgtttttt
    20941 gagatccgga tcttgaataa atcctttgca gataagatct ggtaataagg gagtcataga
    21001 gtttaaccaa gcatccttag tcatctcatt aggatttgct gtgtcctcag cattggatat
    21061 taagggaaat aatgaagtgc ttagcacgag cgtggtagca ataagtaatt tccttgacat
    21121 gatttattgt ccttattttt agagtctcat atatatagta ggacaaaaaa tacaattttt
    21181 cctttattta gtaatttata gttacttgaa actaaatgat tgattcctct ttacttcttc
    21241 ttgccaacac atctatgtgc ttgccgtaca tgctaggcaa tcatggcccc acgggttatt
    21301 gggtaagtta tttaataatt gattagcagt ggcataaagg tgatggaata tttgaattgc
    21361 tcaaaaatta aactaaaata ataaagtgac ggaaaaaaaa taggttaaat catggaaaag
    21421 tccaattttc gcttaaattt tttgtctcaa attttgctct tcactttagg ggtattttta
    21481 ttctgtaatg ggtacgcagc agggaatacc tttattttta atcccaatac attaacctgg
    21541 aaagccatca gtgctaatgg aaaggtgata cgtacaggac gcggatcagg tggaaaacac
    21601 tactgttctg atgttcgcag atcatgtaaa acaccgtctg gcgtttttag agtgattagt
    21661 aaaggtggcc caggatgccg atccagtcgt tatcccctag gtaaaggtgg agcaccaatg
    21721 ccatattgca tgttttttag taaattgtat gcaatccatg gttctccgga tgtgccaaat
    21781 tataacgcga gtcatggttg tgtcagagtg aagcctcatg atgcaagatg gctaagtcaa
    21841 aattttatta aaattggaac gaaagtaatt attaaatcct attaatagca ttaattaatg
    21901 caccataatt ggggattgtg tagtataatt gaaattattg gtgaattata attcattcat
    21961 ttatactcat taattttaga agaagtagcg ttatgattga agtaaatatt tggttatcaa
    22021 ctgctttttt gtttaagaaa cgaattaagc acaaattttt tggcccgctt ttagcttcag
    22081 aggacaaagg ggaaaatgta gggcatgtca attttactat agaaattgat gagcgcacaa
    22141 aaaatagttt tgattttatt gaaaaacacg ggcctgagct gggggctaaa aaaacattgc
    22201 gtgccgtccc aactcaagcg actggacctt ccataacaaa catagaatcg tctcatttga
    22261 aaccaactat ggtaaagagt gatgtggttt cgcatagttt ctggccagaa gggagaccaa
    22321 gtaaatcaga aactataaaa ggtaaaggag tcaaacccaa gtttaaaacc catgaagacg
    22381 atatgattgc tgaagattca gtaacggtaa tggatataat acatcgcaaa tctgctttag
    22441 aagagattgg caaggaaaag atggaaaatc ttgatttgct tgttgaaatt tcagatttgg
    22501 aagtcaatat ggagcaaaga tcacttttta tggatgatct taagaaattg catttggaaa
    22561 aagaggagct gagcaagcaa cagaagcaat taaccagttc acaccaaacc caattaaaag
    22621 atctaaaggg acaattagcc aagctggaac ttcatttgaa taaaacaaac ggacaaatta
    22681 aagctacaga gagaacttta ggctatttga acaaattgga aaatcctgat cttaaaacca
    22741 gagcacaatc gattgagctt actgaaaaat taattaaatt aaaaaaagag ctcgaaacta
    22801 caataagttc tcaacatgag ataagcgaat tgatgtcaaa atctgaactc acttatcatg
    22861 aaggtatgca aaaaattgaa aaaaatcttc aacaggttga gcttgcaatt aaccaccgtg
    22921 aagaaaatct aaggcaattg gatattaaaa tcaatggcag ggatgaaaat gatttaaagg
    22981 aattacaaga agaggctcgc catcgcaaag aatatatttc tcgtaaagaa caattcgtca
    23041 agagtcgaga ttttaccaaa ggtagacatc ctgattacag tgtcacattg ccaactgcgg
    23101 aaagtggttt tacttattat gttgatgaag tcaaaatact gaaagctatg caagaagaac
    23161 gcagccaaaa ttactcattt ctttttaata actgtgccag tagtgctaaa cgttgtttgc
    23221 tagcaggaat agatgaaaaa ttgcgagcca aattaaaaga agctggttta gatagcaagt
    23281 tttttgaaat tagtaaagtg gaaacatgca agtcattacg tgactgggca agaactcttg
    23341 agagtaaatt aacggagctt aattttccac cacccgcacc tacccgaggt gcctaagaat
    23401 aataagcagt tcatccgtaa aaaactaaga tcactgccca agttttaggc agtgattttt
    23461 ttataaatca tcttcatcac caatactaaa cgaagagccg caaccacaag tcgtttttgc
    23521 attaggattg cgtatgacaa attgctctcc ttgaattcct ttaatgtaat caatttccgc
    23581 atcatgtaaa tattggtaac tcattgaatc aatgagcaat ttgacagaag attttccatc
    23641 agaacattgt tgtatgataa tcgtgtcgtc atcattgatt tgttcatcaa agctaaatcc
    23701 atattgaaat ccagaacaac caccacctgt aattgagacg cgtaaattca aattggaatt
    23761 atcttcttct ttaattaatt ctgctacttt atcagctgcg ctgacagaaa aatggatatc
    23821 tgaagtgttt tgggatacgt caacactggc catacttagc tccaaaataa ttactaccat
    23881 tattattcat tatatcaata atgtgagttg tttcgtagtc tcaatccata aattggtatg
    23941 gggaccagag aaaacaaaca gtaccagtac aaaatttgct tccctgttta gcgaataatc
    24001 tgctctatag tagccccacc caagcattga tttttttcat aaaagacgat gaattgtccc
    24061 ggagtaatcg cacgttgagg acttgaaaac atcacatagt gcagattatt atccggagga
    24121 gaaatcatgc aagcttgatc ggtttgccta tatcttgttt tggcaaagca ggttagtggt
    24181 aaatgattct catagtctgc cagccaatga atgggaccac atatcaaacc ttgtgaatag
    24241 agcattggat gttgactgcc ctgtgccacg taaagggtgt tactggctat gtctttatcc
    24301 acgacatacc atggctcatc tgtcgagttt tgtaatcctc caattcccaa accctggcgt
    24361 tgccctaatg tgtaaaacat taaaccatca tgttgaccaa gtgtttttcc tccagtactt
    24421 tttatttctc ccggtttggc aagtatgaat tcattaagaa aagttttgaa tcttttttca
    24481 cctataaagc aaatacctgt agaatctttt ttggcatgag tgacaaggcc cagttgttta
    24541 gcgaattctc taatttgtgg cttggtaaaa tcacctattg gaaaaatggt tttggacaat
    24601 gcctctggtt caacagcgtg gaggaaatac gtttgatcct tttccctatc ttttgcctta
    24661 aataaatagc cgatagatcc ttctatcgta tttttggcat agtggccagt agctatataa
    24721 tctgcgccaa ggtttaaagc atgattcaaa aaggcattaa atttgatttc tttattacat
    24781 aacacgtcgg ggttgggtgt tcttcctttt tcatattcac tcagaaaata agcaaagact
    24841 ctatcccagt attcttttga aaaatttaca gtatgtagtg gtatgcgcaa ttgattacag
    24901 acggcttggg catctgccaa atcttttgct gcagggcaat aatcattgtg atcgtcctgc
    24961 tcccagtttt tcatgaatag cccttccacc tggtatcctt gttctttcaa tagccaggct
    25021 gctacagaag agtctacccc tcctgacata ccaacaataa ctttagcttt cataaatggt
    25081 ttgtatcgct cttttttgaa tcataatcat attggaatgt gcgattttaa caaattaact
    25141 a
//
LOCUS       DATNSN010000031        24917 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.031, whole genome shotgun sequence.
ACCESSION   DATNSN010000031 DATNSN010000000
VERSION     DATNSN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24917
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.031"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2625
                     /locus_tag="VAH76_001065"
     CDS             <1..2625
                     /locus_tag="VAH76_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1385712.1"
                     /translation="MLQTQIIPALTECGEALDKQNVTEALQALNKLPAEKEIGKAKGI
                     GQELRGQIVGVTQELTGNLESLQRAVTTPVVKDAEKMRVRYETLVTDVTKRVTDFEKI
                     KPVNLDSYNKAIADLNNMQQELTLLRNEKIRMHTDKDKAVDFSDIEALEKRLQEAQPN
                     LYPSLVEGTTKGIQELEKIPKSISFDDVKSMTSKINGYLETLEFIRNERVKKHGESTE
                     PLDMSDLDKLKNQLQGVSQHLAQILLNGAKNSLDKIKDPATLEKEGQYIKRCLDHFAE
                     LEKTLDSSVKGMKQKEDFNTYKNSLMEKQEKAYPEMLQLQYKSEALITQLRNLCNIHH
                     DNLAKTREEQQQLLNKTGGKLDGFFSQITTAISQAEAKKANELARFKTELNNDKNDVS
                     QLIQFLVKKKPSELEENLGISKENAEQLHGLLKQLDAKATPIDKLQENTRLIDEISTK
                     MGSKPVSPEHVAATKAREYYQFLLYGTTQKIGNFEKVKPLDPESYKKAMSDLNNMQEA
                     LQFLRSEKSRIHDDKEKAVEFSDIEALEKRVKNAQPKLLTALLDKTTRDISALVKIPR
                     KLTFEDIKSMTTKHNSYLETLELIRNERIKQHGESPELLDMSDLDSLKGQLQTFNQNL
                     TGMILNATKDGLDRINNRANFNEGEPYVKTCLDLLTELEKTLDSSVKGMKQKEDISAC
                     RNSLLDKQEKANSGMLDLQSKSKDLVTQLRDICKIHHGNLAEARRAKLHSLDNQEKGL
                     LGGLWSVTNKLGVTTDTVGIERMQIKMKEQTLRAFKTELTNDKLNTDQIIAFLANKKP
                     SELEEGLGISKENAEELHGLLSKLTSKMTSKIEIEENTQLIDEISTKIGTEPVQLELT
                     RTVDEDESDTYRRESGYI"
     gene            complement(3008..5275)
                     /locus_tag="VAH76_001066"
     CDS             complement(3008..5275)
                     /locus_tag="VAH76_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385713.1"
                     /translation="MPKFKTDEERMKHPQAKLIPSSMWNDNELFCETLNDTVLSLMKV
                     TEKDLMYRLTNAIPKLNNLWLKKQAWLAIALSHPNLELSMLEQVAKLLGLEDSKIFSL
                     LAILGKVHLLAEFVKRHAQSHILELIASNSFSVYRKAAENGHIDVLDYLETLVKPKQV
                     IQMIRAVDFSAYRDAARNGHLDVLKNLEGKAPDLVLSMIKAENFYAYRLAAARGNIEI
                     LKHLEANVPNLITDMVKAEDFYAFRKAFENGHIEQCKSLLSKSNLCFAYAEMHMREYG
                     EQIIEPFIDQLLLTLHRDSLNTPAHGVFDVKDPEQAKICFYMIRNIIRRNDRDFDDQI
                     RFLLSIPSVRDLAHREITVGLPNELVRLALTTGNQQAASILLNIPEVRILSEQNNYYY
                     ADIQGQLDLARLAKDRESAMTALTKGEQKRLNAAIEYYRPALKEHGVDKLMNDLREQL
                     RQRYESKPALIVSDDGLEIKLPMDFSEFQKLNLNKNEYQQALKAYYQHKDHTAWRYLA
                     KPNLWMNNEASYVYFDKKRGERWSTFEEYQPLIVLFWLAATDNSTPPIDGHTFQSRLD
                     HFIDELALIGRAHNWDQTRINEKQQEEEYDDLTGDKPSCFSGVKRRLFQSVLGHPLIT
                     ILTEDMILEEIRNFARDHFQSQINEENRHMFKEAFEDYIVNTNDIEEDNKKLLLTLNI
                     SKEKLQQFEFNLVNKYGAQYAEDYFFQKLVRTKLSLASDGTEFFYQSHALSLDGIVGF
                     YKLVNGSTLIRPDFR"
     gene            5511..6431
                     /locus_tag="VAH76_001067"
     CDS             5511..6431
                     /locus_tag="VAH76_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385714.1"
                     /translation="MAKRYEKRIDWQRIYHKYRRSNVKKRSQLLNELCDLTGMIRKYL
                     IRKLNKKKVKSSKHRGAKPTYEAEIYLPIIKPIWLAADQIRGKRLKEVLQDCLHFYEE
                     EFGDLDEIIRFKVKQISSATLDRLLKPLKANYKGRGISGTKPGTIIKNQIPIKTNQWE
                     ETKPGFMEADTVAHCGISLEGNFVWSLTMTDILLTWTENWAVWNKGAYGVGNAIDDVE
                     ANLPFEIKGFDCDNGSELLNYYLIRKFAERPQETAIQFTRSRPYKKNDNAHVEHISVI
                     VKLHAKSPAKDEPQWATVSASKKPGFSSFH"
     gene            6953..20764
                     /locus_tag="VAH76_001068"
     CDS             6953..20764
                     /locus_tag="VAH76_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385715.1"
                     /translation="MPKYTQEMANLFANLEPHQIELFVGMLSVDAIKIIRTEVLPQIK
                     DTERRIFLKQTLAESEGHLKQSSKKSQASQQLENISDQLKNLYDNLTTVVLWKIADDD
                     AQKRFIAEANDEQLLILKRKTSPYKDEHSKTFNLAIENEIERRKQENLGHSSISKKEN
                     LQWEANVWLSIIDKIKKVNSDLEIERREQAEKQVKPVQPQGPTVVEKSWWSNFKESVS
                     NLFSSSKPVPTPYKLEISENEKIEIDGLRKEGAQRIANNTGVVKVGEAEELSAEGKKK
                     AMMSRFGMVMASQMVAGTKSDNHRILYNVLESLAQPEDDDDALEKIQNAIKPVIPDTP
                     EQPNPLKGKHACATGGNPEFVTAFESEVVQKLLSSPEHFEQYGVRLSEKEKEKVLHYH
                     ENMERSRDINNVITSICYSWGGYEKDTVQKGYKKSENFETKQARYEERLGLASKGLQE
                     ACAKLQDGQSLYLETGVENHAMQLVIKKVGNEFKLSTYDSSGALENTALSQSLFGLFK
                     LSRLRSTGAMRRNAYSFTVPQEKLISEQGLNYLSGLIRSNSMAGWAQTHINQNLQTAT
                     REEMSHMNWFQRLYALREARAKYKVYMDHFASLASPDSPPKFDDLLQHPQNTSNCFAK
                     KAQSCELYELGKPVYKKFRLAMLLEQKDKLLSDIEGQDQFVSMEYRTMLKNIEPEYLS
                     PTELHEISMRLCEVKEPPTKGYYEKFFQTLLDAREKLVEANNDDNKLAIQRIDEKIAS
                     HAQSYYNYLSKGNRQSEIQQIFSSEVCSQGNSYNWKTGAMPFKTGLDGKIDPTALGKI
                     SEYASAQAWKASMQMLNHQIKKLSVNERYISSSKERLSHASTREASGKVTLKDLENAN
                     IVNFTTGFTRQEETKIEINVGGKRREIDKATFFELIVKNKESLTNPKVISLLDSLRNT
                     SPAIEKRYMKEVYPTQRKAFESKVEQNSSKSGKLLEETISSLKKHQMQIEPMIQRSDE
                     NIRLITDEIDKEKLNSNGKRSIKLDNLELRLALLKIENQELKDLEASVSTKIERLELE
                     HGSIKGKIAKAEEILKLLQTPGALKSINEINHAFLDARAELESVERELNSTKKEFEVN
                     DIENHINDRMVQINHYRQKIVGRHLQANAEYRILDELSAGRMGTEKDRNRNMYRQTES
                     ERSAFNKQGKNTFLPGSVFQEIQSLNQKLKTKVNEIAERQIILGTNISDTVSLASLES
                     ANDKYMQMDRQAQELSKKSIPQEIKEEWVREMFNIWLSHESKDRLYHLQKKYGDDPSV
                     GINQAFHHFIEQKANIKYAALKEAGYEIDLDKSKIVQMGWKPISEQELGEYHEKRKQA
                     ASKLMELFKFKRVLEKLEEGIQSKEKTVTPEPVTLTSEDLSAQHEVSLVTKGFVPPEV
                     TFLSQSQREALDGSTLKFLRENPTPQPKGVSREECEQYYKEVIAHLHKLKMHSALENK
                     AQRIAEFCHSSASGIFDLPYPPSQELALEIAHNIIDSYRDDENLINDSFLKLENNERA
                     QILSALIKLSLSQIEVSESQKTPVNSKFYSTIKQWERLLIPVDEAISRKISMLAPGGT
                     EPTNMDLLREVDVALHQSPVSLEGLYEGQKGLQAAITTYGMEKIVEGADANLRKLADR
                     LGMRNGEALLASQFINYYSNTKLLLSSDGINSTQGRELFSRALLDAFQNATPSEKQQL
                     LAFIQKLEFNDEVEPCTLKTSHQAFIDEALLRCSNLDPEAFREYSVAKVEEHSPETLL
                     SFIDTLTKESNEDGTDKLFGFAKEINILSLQIEHALNSKPVDASRLDVLYSKVICSNL
                     AYQLLLDQVTEETAANLGENFEYTREMALAQANINKLQDNLINFAGNLDRESQFFSVF
                     SKYVNEKKFDGPPILAQSASRSDIPGFISLGGNRSLDVLHGAVYVGNNRLGMMPAHLQ
                     SHIAVNELGLNQLPFRPLGGGYAYSEGKEIKAFIMPSEDGTVTIQRELMTFDGTSEML
                     QYISPEKMDSVPMALKRRLNAEHFFIDSKGTIHAYTANFKPILKLAKHEELWTGELLD
                     HNGNKTTISLDSEGNTPIIQNLSRVFPQGEMISVDSNTVYIPSISKYVISDKSGSNYF
                     ISDNLIDSTSRKHLTIMDQGSAHTERVLTESESQEVLSLQKQIDKLKEEHALITKSDL
                     VSKQNKSKLEKKIKECESRIRDITTPENFVFVSDSKQIQSLEQEHLRLRKEMQQAYTD
                     FREGGKKDKEKLSTYYEKAKEDYLQNKKALQKADSQANYLRVFDVKEGTLHAKDFASI
                     LYVGLIPGKTDVLTQSLASSVPKFPLKLNELEDLRNLKVQYKDKSPISKEEQLALIML
                     IGTELQHHILERSAAATGKLRDWDRATYNKLLTEFTEEVKRANELHGKPPLSQFGELW
                     RAIQSEFSSDKELQEIFTKSVQLVDTGEKKPININTKTTNMPIETMGARTLVEFKLHD
                     NPQSLIDPEQVELERSLQGITGFESSVQAQEDGYYYENYGLFNTNTFEKLFSVTYQHA
                     GIGGLTKEQVTDLFKLMEKEGWIRKVPGMEDKNKYQITRHPSEFYSSAKIAGYLNELG
                     FERNQSNAISERLEIFLYQTAVNGGSYKVNEKNREELIQKVTEAQDKCNLEYLEALDK
                     IESTLAKASSKITFADLNAAYLLNDYSSILADFPEKDRAQVEIVLNNGMTRLLYYKTE
                     LDHLNDVQFKFKIGQDSTAVAMLHTRRNYQLDKLLDSETTLDDKSTKEEIQKVSEERK
                     MQRAFLLFESEFGHRCNARQVNIFRGLLLDDATDPDKIDSAQARMGFGKTSLLPLVAL
                     YKTGDKLVRFIVPKSALETNTADMSTTLTNILGRRAVKDDFQRYRIATDPETGMGENS
                     PCLKSLQDAKADLQKRLALYHRVRDNKEVLVQAPSVRNSMECQAKIFLDMLMKVTDEP
                     LQKKELMACISLLNEIRSLTTVSVFDELDATQDPATTEVNYTAGEKISLDTAEIYPLE
                     VITQTIGATEDKSIENLAKVLLNKFGIEDVDESIFKYITSLKEKQPPSVTPSNSTEIY
                     LIRAILSDPVMLSIFTEKEPGTDFGVWFENAKDGSKLYDYEALKTGRDDSRTPLLIAI
                     PYSAANTPKPQGSRFDNPEVTAITTMLYYLDPRTEINELPHLEFLIDSMRKGIGEAPF
                     LDSSGEHLEPEFKKLFNEISALAEIEDPLIRNEERKKYFAGLEERIKEGEIAPTALRK
                     MLARTIIQEQVKFDAGKANSNRYEQGTTDDVVIGFSGTAGDTSSHFKENMLDPAADGN
                     MTLGIMGRKNCQATTALDTTSFAEKGEDYNTVLVKQLAASFTSNTRTLIDVGGLCKAS
                     NRAVAKEIALQLKESSDSTLKNLKGVIFYDDVTNMKKLLVLEESGREKIVDLTSDMVA
                     ESDQKGSYFTYYDQSHSRGADIKQMDKAHAVLTMNFNVTNNDYKQAIMRMRKIVDKSL
                     GQSFSTAVPDNVREKIIDELNLGKEHTLTGNDIAVWLRQKELKNNLNNISVFTMELDS
                     IIKNAILQQQGKITQLMSQSPMTEEQLEAFRECIRELNNISPFISGGSTDLIEKYGKV
                     YGTVKKEDFVKDLHESFGKRLNHVFDIVNRARENIKLPLTTESDRAPYVEMEKRIVQK
                     REAQLSPEFVIPSSSSALAEAQSETENQSESQSQSQSQSQTQTHSFSEVANEEVVVEA
                     KLRKHEVLDEPASIAYLSESEKIDKLVLASQTPHMQHLFKDEDPIRCSPAYSVSGEKD
                     HILPPVRYFIAREEGNPKIILINQDEANLFKSSPVDPWSLYDVRLKKGESLEPMVGSS
                     IASLNESILKKVSLASFRYELKGQDIESLAQSLEGICTPNQLHPSLSMKYETSKNIAK
                     DGGVFSLPKWGFYGEKQQDIQFAIEQTQVKFKEGKYEKRGVTVSFGKGDERAEVFISS
                     KLNKRILDEVGKQERAEVPVGHPKLKAVIEQVKKEHDSAMKERSELRSDLKDLKSRKN
                     KVIQDADQKISELEEKKRKAIEQARVDISKGFEDGMKNQINRRKYFQSYFAKNIGAMC
                     RLRVGDNKDFGVQVFGKTFPNLEKAIAHAVTQLYAEHKKKAMTSEELNSRVDFYIEEI
                     VKAAEERFHSVGRYRDKTTTALETLHAASKEATPMSKEKIELLLKGELQDLKQAWMKG
                     DKWDKLVAALEEVTNITGKNLSIDEFHQKTLEALQKFASENGEDLDKTGIVPRLFSSL
                     TLGEKPRNKDGFMAGFLNEDPNKVRNHAAIRSDIANFIKRGFAGNTNTPSEEQREFFT
                     AVNSLVNSKINRHNYDVPATLLDFESPISLERMNEGIKQTLTTQNVRTVKKDTDSMAR
                     ECSAYLVGRKALENILDHYKPLENRSSQIDPVEGQEAKFFSANFERLTKLDTEVLDIQ
                     RQIDEVKSQKKAHLGQLEEEHVVLRDKLQKNGEKVSELKGEISALNKLISGIKKVLSV
                     FTNHQVKIEQDDPIDFLDTHLDLGAIIQSEVSATATLTFTPPEFYDLEKDMQAQFLNM
                     HGLEETTERRSEKSFDEAATLVRETAGKFQERESLTVGLKLASSKETELVDKVQLEQE
                     APKVERASSFKQQLSQIRKDQVEALITPIVEQNPTQAFKNKLKHEMNKDEEVVMTVPV
                     HETTNSFKDKIKKIRDNETTAPDIALTGLTPNPKD"
     gene            21045..22021
                     /locus_tag="VAH76_001069"
                     /pseudo
     CDS             21045..22021
                     /locus_tag="VAH76_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            22252..>24917
                     /locus_tag="VAH76_001070"
     CDS             22252..>24917
                     /locus_tag="VAH76_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1385716.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGDITSGSIYNGVPTIDTGAL
                     NRQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKTSGEGYQNILAAQLPMGS
                     TLSVIPNGSNLNRGLCGYWVASAGLRAQQALNQHNPPTLLNLGQTITDEMRNELDHDG
                     YRKITGWLRAVADEFPEGDPQLDGKALRENTEKDLKIEIPTLILPGKDTSPKETPTTP
                     TAPQVAPKYSLDSKLLENDDDVLETINYVHKEYLNKDYPGPLKNPKDPKEGRIPPDEQ
                     SRINHGLAHTVRTMACAEVMIEEARKAKLRGETLGKAKNGQTLADVTPEEMKKILIAQ
                     AFFVVGRDDERSGYDEFHKRNFYAEYHEKSEQAFRKYVEDNQLIGKIFKDQKEVDFYA
                     AIILDKDHDWTATPAHILINQGHMVDLMRVKTPSEVTLERAFNALKNTVGSKGAEAVL
                     KAHRDFFFATGAVVPLVNPEAIDDPSRGGPYENPYNGEKYVIVEGKEPKSTKDLPKPV
                     GRNYQLKDNERFLTIKEYYAFPDVQQIYPGYKTRLEGTSYYLPTPFARECERDPAKCL
                     GAIQKARSKLQTDAIKNGFQSSSDKARRQPNVDEIAAARIIQQILANPDCIHDDHVFL
                     NGQKLEEKFFRDLLAKCEMAVVGSLLNDTDIGNIDTLMRHERDTEFHATDSDAVPKKI
                     GEYWVNDQNKNNKSNNITQKKHDLIFLMQNDKWYFSRVNAIAQNRDKGSTFKEVLITT
                     LMTPLTSKALVDTSSAPAPKRLFRGLNLSEEFTNKLINQTNAIIANTENTLFTDLSAE
                     TFKQIKLNDLSQMSGRTNASTTTNIELCRTIWDSNVIFEMLDPDGLLHPKQVGRHGAG
                     TESEFSVYLPEDVALVPVKVTLDSGKTQNGENR"
BASE COUNT         8800 a         4309 c         4861 g         6947 t
ORIGIN      
        1 atgctgcaaa ctcaaattat cccggctctc acggagtgtg gtgaagcact ggataaacaa
       61 aatgtcacag aagcattaca agcgctcaat aaactgcctg cagaaaagga aattggtaaa
      121 gccaagggga taggacaaga attaagaggg caaatcgttg gtgttacaca agagctaacc
      181 ggaaatcttg aatccctgca acgcgcagtc actacccctg ttgtaaaaga tgctgagaaa
      241 atgagagtac ggtatgaaac ccttgtcaca gacgtgacaa aacgagtcac cgactttgaa
      301 aaaatcaaac cggtcaatct tgacagttat aacaaagcca ttgccgattt aaataacatg
      361 caacaagaat taactcttct gcgcaatgaa aaaattcgca tgcatacaga taaagacaaa
      421 gcagtggact tttctgacat agaagccctg gaaaagagat tacaagaagc acaacccaat
      481 ttatatccct cgcttgtgga aggaacaaca aaaggtattc aagagcttga aaaaataccc
      541 aagtcaatca gttttgacga tgttaaatca atgacatcca agattaatgg ttacctggaa
      601 accttggaat tcattcgtaa tgaaagagtt aaaaagcatg gcgaatccac tgagccactg
      661 gatatgtctg atctggataa attaaaaaat caactacaag gcgtgagtca gcatttagcc
      721 caaattttat taaatggagc taaaaactca ctcgataaaa tcaaggatcc ggctacactc
      781 gaaaaagaag gacaatatat aaaacgctgc cttgaccatt ttgctgaact tgaaaaaact
      841 ctggatagtt cggtaaaagg aatgaaacaa aaagaagact ttaacaccta taaaaactct
      901 ctaatggaga agcaggagaa agcctatcca gaaatgctgc aattgcaata caagagtgaa
      961 gcattgatta cacaattacg caatctatgc aatattcatc atgataattt ggccaaaacg
     1021 agagaggaac aacaacagct actgaacaag accggaggaa aattagacgg tttctttagc
     1081 cagataacga ctgctataag tcaagccgag gcaaagaaag caaatgaact tgcaagattt
     1141 aaaactgaat taaataatga caaaaatgat gtgagtcaac ttattcaatt tctggtaaag
     1201 aaaaaaccat ctgaattgga agaaaacttg ggcatatcga aagagaatgc tgagcaatta
     1261 catggactgt taaagcaact agacgcaaaa gctactccaa tagataaatt gcaggaaaac
     1321 acaagattaa ttgatgaaat atcaaccaaa atgggtagta aacccgtctc gccagaacat
     1381 gttgccgcaa caaaagcgag agaatactat cagtttcttc tatatggaac gacacaaaaa
     1441 atcggtaact ttgagaaggt caaacctttg gatcctgaaa gttataaaaa ggctatgtcc
     1501 gacttaaata acatgcaaga agcattacaa ttcctacgca gtgaaaaatc ccgaatacat
     1561 gatgacaaag aaaaagcggt ggaattctct gatatagaag ccttggaaaa acgagtaaaa
     1621 aatgctcaac ctaaattact gactgcactc ctggataaaa caaccagaga tatctcagca
     1681 ttagtaaaaa tacccaggaa attgaccttt gaagatataa aatcaatgac aaccaagcat
     1741 aacagttacc tggaaaccct ggaattgatt cgtaatgaaa gaatcaaaca acacggtgaa
     1801 tcccctgaac ttctggatat gtccgatttg gatagtttaa aaggtcaatt gcaaaccttt
     1861 aatcaaaatt tgactggcat gatactaaat gctactaaag atggtctgga tagaatcaat
     1921 aatagggcta actttaatga aggagaacct tacgtaaaaa catgtcttga tcttttaaca
     1981 gaacttgaaa aaacactgga tagttcggta aaaggaatga aacaaaaaga agacattagc
     2041 gcctgtagaa attctctcct tgataaacag gaaaaagcca attccggaat gctggatttg
     2101 caatccaaga gtaaagatct agttacccaa ttacgtgata tctgtaaaat tcatcacggt
     2161 aatttagctg aagcaagaag agccaagctt cattcacttg ataatcagga aaaaggatta
     2221 ttaggtggat tatggtctgt tactaataaa ttaggtgtta ccacagatac agtaggtatc
     2281 gagagaatgc aaataaaaat gaaggaacaa actctaagag cgtttaagac agagcttact
     2341 aatgacaagc ttaatacgga tcaaatcatt gcctttttag caaataaaaa accatcagag
     2401 ctggaagaag gtttaggtat atcgaaagaa aatgccgaag agttacatgg actgttaagt
     2461 aaactcacta gtaaaatgac ttcgaaaatc gaaattgaag aaaatacaca gttaattgat
     2521 gaaatttcaa ctaaaatagg cactgaaccc gtgcaactcg aattaactcg cactgtagat
     2581 gaagatgaga gtgataccta tcgtcgagaa agtggttaca tttaggtgct tgtttgctgg
     2641 ttaaatgatt gaaatcgaca gcagatgaga aaataaagac tacgggcact ttattttctt
     2701 atcgctaaaa ttttgtgata ctcaaggccc atggagcccc gataatccag gcacttagcg
     2761 attaaatttt tctattttat ttggagtcat gcgccagcag cttcagcaag tcatcgtggg
     2821 aaattgtgag cctgacttat tccaaaaact ctttgttaga gctgcttcac gcacaaaaat
     2881 taagataaga cttaatcgaa tttttaacag tgacaaagtg ggaaaaccca aggggcatca
     2941 gacctttcgg cgtgtttatc aatcgattta aatttgctag aaatgtgtgc tcaacaattt
     3001 tgatgcacta cctaaaatct ggcctgatta gggtactgcc attaaccaac ttatagaatc
     3061 caacaatacc atctagtgac agggcatgag attgataaaa gaattctgtt ccatcggatg
     3121 ctaaacttaa tttagtacga acaagttttt ggaaaaaata atcttctgca tattgagcac
     3181 catatttatt cactaagtta aattcaaatt gctgtagttt ttctttagaa atattcaacg
     3241 ttaaaagaag ttttttgttg tcctcttcta tatcattggt attaacaatg taatcttcga
     3301 acgcttcttt aaacatgtgt ctgttctctt cattaatttg tgattggaaa tgatctcgcg
     3361 caaaatttct tatttcctct aaaatcatat cttcggttaa aatggtaatt aggggatggc
     3421 cgaggacgga ttggaagagt ctgcgcttta cgcctgagaa acaagaaggt ttatcgccag
     3481 tcaaatcatc atattcttct tcttgttgct tttcattaat tcgagtttga tcccaattgt
     3541 gcgcgcgtcc aatcagcgca agttcatcaa taaaatgatc gagtcgcgat tggaatgtgt
     3601 ggccatcaat cggtggtgtt gagttgtcag tagcagccaa ccaaaacaag acgatcagtg
     3661 gttgatattc ttcaaacgta gaccatctct ctccacgttt tttatcgaag tatacataag
     3721 acgcctcgtt gttcatccac aggttaggtt tcgcaagata acgccatgcc gtgtgatcct
     3781 tgtgttgata atatgctttt agagcctgtt gatattcatt tttgttgaga ttgagttttt
     3841 ggaattcaga aaaatccata ggtaatttaa tttcaagtcc atcatcactg actatcaagg
     3901 ctggtttgct ttcatatcgc tgtcttaatt gctcacgcaa atcattcatg agcttatcaa
     3961 caccatgctc cttcaatgct ggtcgataat attcaattgc tgcatttaga cgcttttgct
     4021 ctcctttagt cagtgcagtc atagccgatt cacgatcttt agcaagtcga gctaaatcca
     4081 attgtccttg tatgtctgcg taataataat tattttgttc agagagtatt ctgacttcag
     4141 gaatatttaa aagaatggat gcggcttgtt gattgcctgt agttaaagca aggcgtacta
     4201 attcatttgg tagaccaact gtaatttcac ggtgtgctaa atctctgact gatggaatac
     4261 tcagtaaaaa acgtatctga tcatcgaaat cccggtcatt acgacgaatt atgtttctta
     4321 tcatataaaa gcagattttt gcctgctctg gatctttgac atcaaaaaca ccatgagcag
     4381 gagtattcag actatcgcga tggagcgtta agagtagttg atcgatgaat ggttcaataa
     4441 tttgctcacc gtattcacgc atatgcattt cggcataggc aaaacaaaga ttactttttg
     4501 agagtaggct tttgcattgt tcaatatggc cattttcaaa agccttacgg aaagcataaa
     4561 agtcttctgc tttcaccatg tctgtaatga gatttggtac atttgcttct aaatgcttta
     4621 atatctcaat atttccccga gccgctgcaa gtctgtatgc ataaaaattt tctgccttta
     4681 tcatgcttaa aaccagatca ggagctttac cttccaagtt ttttaataca tcaagatgac
     4741 catttcgggc agcgtctcga taagcagaaa aatctactgc tctaatcatt tgaatgacct
     4801 gcttgggctt taccagtgtc tccaaataat ccaacacatc tatatgacca ttttcagctg
     4861 cttttctata tacagaaaag ctattgcttg cgatcagttc caaaatatga ctttgggcat
     4921 gtctttttac aaactcagct aaaagatgta ccttccctaa aatagccaat aaagaaaaaa
     4981 ttttgctatc ttctagtcct aacaatttag caacttgttc caacattgat aattccaagt
     5041 ttgggtggct taatgcaata gctaaccagg cttgtttttt tagccatagg ttatttagtt
     5101 ttggaattgc attagttaaa cgatacatta agtctttttc tgtgactttc attagtgaaa
     5161 gcactgtatc atttagagtt tcacaaaata gctcattgtc attccacatt gaggaaggaa
     5221 ttagttttgc ctgcggatgc ttcatccgct cttcatctgt tttaaacttg ggcatattag
     5281 ctcttacagt taagaataag gttatgtcat tgaattattg ggaaaaatat cggtgccata
     5341 tagatttttt ctttaaaatt cacgcccgaa ttgtataagg aaacaggata ttagcaccga
     5401 tagtgaaaat attctgtgat attgatgatt tttgcaaggg gtggtttaaa gaaatgtgtc
     5461 attatcgtgt tgaatcacat taaaggtaac ccgaaattgt ggagctaacg atggcaaaac
     5521 gctatgaaaa acgaatcgat tggcagcgaa tatatcacaa atatcggcgc tctaatgtaa
     5581 agaaaagaag tcaattattg aatgaactat gcgatttgac tgggatgatt cgcaagtatt
     5641 taattcgaaa gctgaataaa aagaaagtta agtcttctaa acatcgagga gcaaaaccga
     5701 cgtatgaagc agaaatttat ttaccgatta taaaacccat ctggttagcg gcagatcaaa
     5761 ttcgcggtaa acgtttgaaa gaagttttgc aagattgtct tcacttttat gaagaagagt
     5821 ttggcgacct agacgagatt atcaggttta aggttaagca aataagctca gccacattag
     5881 acaggctatt aaagccactc aaggcgaatt acaaaggacg agggatatct ggcacaaaac
     5941 ccggcactat tattaagaat cagattccaa ttaaaacaaa ccaatgggaa gaaaccaaac
     6001 cagggtttat ggaggcagat acagtggcgc attgtggaat atctctagaa ggtaattttg
     6061 tctggagttt aaccatgact gatattttat taacttggac agaaaattgg gcagtttgga
     6121 acaaaggggc atatggagtg ggcaatgcca ttgatgatgt tgaagctaat ttaccttttg
     6181 aaatcaaagg gtttgattgt gacaatggct cagaactttt aaattattat ttgattcgaa
     6241 agtttgcgga aagacctcag gaaacagcga tccaattcac tcgttccaga ccttataaaa
     6301 aaaatgataa cgcacatgtc gaacacatat cggtcattgt taaactccat gcgaaatccc
     6361 ctgctaaaga tgaaccgcag tgggcaacag tatctgcttc taaaaagcca ggcttttctt
     6421 cattccattg atttgttttt atcggaatat gtttttttaa aatggtaccc gattttgttc
     6481 cgctcaaccc tcgtttacca ttaacacgtg aactggataa catacgatct attgtagagg
     6541 cactcatctt taataagccc tcatatatat tgggttgggc gtccctgtct ttttttatca
     6601 gttttattag gctgtttctt ttgatttaac aatcgtattg cgcgtttttt gtggtaaccg
     6661 cttgcagtac agagctcatt taatatcgtg ctcttttctt tccgggtagc agcatgatac
     6721 cgcctaataa tattgtccaa ataaagatcg attgaaagct tccccatttc tatgtccttt
     6781 ttttcttagg taacattatt tatgaggcaa ttcgaacgaa tgttgattag aaaaatattt
     6841 gttcatgtgt actataatta tctataaaga acaaaaattg cttccaaatg aagcactaaa
     6901 gtcgtgaaaa actacaatgc cgttaaggcg ttattgtaaa gagagtctat aaatgcctaa
     6961 atacacccaa gaaatggcaa atttatttgc taacctagag cctcatcaaa ttgaactgtt
     7021 tgttggaatg cttagcgttg atgcaattaa aataatacgc actgaagttc ttccacaaat
     7081 taaagacaca gagcgaagaa tatttttaaa gcaaactttg gctgaatcgg aaggacatct
     7141 taaacaaagc tctaaaaaaa gtcaagcatc tcaacagctt gaaaacataa gcgaccaact
     7201 gaaaaatttg tatgataatc tgaccacagt agtcttgtgg aaaatagctg acgacgatgc
     7261 ccaaaagcgt tttatagcgg aagcaaatga tgagcaatta ttgattctta agcgcaaaac
     7321 aagtccttat aaagacgagc acagtaaaac ttttaacctc gcaatagaaa atgaaataga
     7381 acgacgaaag caggaaaatc ttgggcattc ttccattagc aaaaaagaaa atttgcaatg
     7441 ggaagctaat gtatggctaa gcattattga taagatcaaa aaggttaatt ctgatcttga
     7501 aattgaaagg agagagcagg ctgagaaaca ggttaagcca gtacaacctc aggggcctac
     7561 cgtggttgaa aagtcatggt ggagtaattt taaagaatct gtttctaatt tgtttagtag
     7621 ttctaaacca gtgcctacac cttataaatt ggaaatatca gaaaatgaaa aaatcgagat
     7681 agatgggctt cgcaaagaag gggcacaacg tatagccaat aatactggag tcgtaaaggt
     7741 tggggaagca gaggaacttt cagcagaagg taagaaaaag gccatgatgt ctcgctttgg
     7801 gatggtaatg gcatctcaga tggtggccgg cacaaaaagt gacaatcatc ggattctata
     7861 taatgtcctg gagtctttag ctcaaccaga ggatgatgat gatgctcttg aaaagataca
     7921 gaacgcaatt aagcccgtaa tacctgatac accagaacaa cctaacccac tcaaaggaaa
     7981 acatgcttgt gcaactgggg gaaacccaga atttgttaca gcatttgaat cagaagtagt
     8041 tcaaaaatta ctaagttcac ctgagcattt cgaacaatat ggtgtgcgtc tatctgaaaa
     8101 ggaaaaagag aaagtattgc attatcatga gaatatggag cgctcaagag acataaataa
     8161 tgttataact tctatttgtt attcttgggg ggggtacgaa aaggatacgg tacaaaaagg
     8221 ttataaaaaa tctgaaaact ttgaaaccaa gcaagcgaga tatgaagagc gtcttgggct
     8281 agccagcaaa ggcctacaag aagcatgcgc caagcttcaa gatggccagt ctttgtattt
     8341 agaaactgga gtagaaaatc atgcaatgca acttgtaatt aaaaaggtag gaaatgaatt
     8401 caaattgtct acctatgatt cctccggtgc tttagaaaat acagcgctta gccaaagttt
     8461 gtttggtttg tttaaactgt ctcgtttaag aagtacagga gcgatgcgac gtaacgctta
     8521 ctcatttact gttccacaag aaaaattgat ttctgagcag ggattgaatt atttaagtgg
     8581 attaatacgt tccaattcaa tggccggctg ggctcaaaca catataaacc aaaatcttca
     8641 aactgcgaca cgagaagaga tgagccatat gaactggttt cagcgtttgt atgcattaag
     8701 agaagcacga gcaaaataca aggtttatat ggatcatttt gcatctctgg cttctcctga
     8761 ttctcctcca aaatttgatg acttattgca acatccacaa aatacatcaa actgttttgc
     8821 caaaaaagca caatcttgtg aattatatga gttgggtaaa ccagtctata agaaatttag
     8881 acttgcaatg ttgctggaac agaaagataa attgctaagt gatatagaag gccaagatca
     8941 atttgtgagt atggaatata gaaccatgct caaaaacatt gagccagaat atctctcgcc
     9001 aactgagtta catgaaattt cgatgcgttt atgtgaggta aaggagcctc ccactaaagg
     9061 gtattatgag aaattttttc aaacgttact tgatgccaga gaaaaattag ttgaagccaa
     9121 taatgatgat aataaattag ctatacagcg tattgatgaa aaaatagcaa gccatgctca
     9181 atcctattat aactatttaa gtaaagggaa tcgccagtca gaaattcagc aaattttttc
     9241 ttcagaagta tgtagccaag gtaactcata caattggaaa actggggcaa tgccctttaa
     9301 aacaggcctt gatggaaaaa ttgatcccac tgcattagga aaaattagtg aatatgcatc
     9361 tgctcaagct tggaaagcat ccatgcaaat gctcaatcac caaattaaaa aacttagtgt
     9421 gaatgagcgc tatataagct catcaaaaga acgtttatct catgcaagca cacgagaagc
     9481 ctccggtaaa gtaacattaa aagatctgga aaatgcaaat attgttaatt ttactaccgg
     9541 atttacaagg caggaagaga ctaaaattga aatcaatgtt ggtggaaaac gtagagaaat
     9601 agataaggcc accttttttg aattgatagt taaaaataaa gagtcattaa ctaatcccaa
     9661 agtaattagt cttttagatt ctttacgtaa tacttctcct gcaatagaaa aacgatacat
     9721 gaaggaagta tatccaacac aacgtaaagc atttgaaagt aaagtggaac agaattcaag
     9781 taaatccggt aaattgttag aagaaacaat tagttctctt aagaaacatc aaatgcaaat
     9841 tgagccaatg attcaacgtt ctgatgaaaa tattcgtctc attactgatg aaattgacaa
     9901 agaaaaacta aacagtaatg gaaaacgttc tataaaatta gataatttag agttacgttt
     9961 ggcactactt aaaatagaaa atcaggaatt gaaagatcta gaagcttctg tttctactaa
    10021 gattgaaagg cttgaattgg agcacggttc tataaaagga aaaatcgcta aagcggaaga
    10081 aattcttaaa ttgctccaaa ctccaggtgc attaaagtcg attaatgaaa taaatcatgc
    10141 ctttttagat gcacgggcag agcttgagag tgtggagcga gagttaaata gcactaaaaa
    10201 agaatttgaa gtaaatgata ttgaaaatca cattaatgac cgtatggtac aaattaatca
    10261 ttaccgacaa aaaattgtgg ggcgtcattt acaagctaat gctgagtatc ggatattgga
    10321 tgaattatca gcgggtagaa tgggaacaga gaaagaccgc aatcgaaaca tgtatcgtca
    10381 aacagaatct gagagatctg catttaataa acaaggaaaa aatacatttt tacccggttc
    10441 agtatttcag gaaattcaaa gcttaaatca aaagcttaaa acaaaagtta atgaaatagc
    10501 tgagcgtcaa attatattgg gaacaaatat ttcggatact gtttcactgg cttcccttga
    10561 aagtgcgaat gacaaataca tgcagatgga tcgccaggct caggaactta gtaaaaaatc
    10621 cattccccaa gaaattaaag aagaatgggt acgtgaaatg tttaatatct ggctaagcca
    10681 tgaaagcaaa gaccgtttat atcatctcca gaaaaaatat ggagatgatc ctagtgtagg
    10741 catcaatcaa gcatttcatc attttataga gcaaaaagct aatatcaagt atgcagcatt
    10801 aaaagaagca ggttatgaaa tagatctgga taaaagtaaa attgtccaaa tgggatggaa
    10861 acccatcagc gagcaagaat taggggagta tcatgaaaaa cgtaagcaag cagctagtaa
    10921 acttatggaa ctctttaaat ttaaaagagt tctagaaaaa cttgaagaag ggattcaatc
    10981 taaagaaaaa acagttacgc ccgaacctgt tacacttaca agcgaggatt taagtgcgca
    11041 acatgaagtc tcattagtaa ctaaagggtt tgtgcctcca gaagtaacat ttttatcaca
    11101 atcacaaaga gaggcattag atggaagtac cttaaagttt ttgcgtgaaa atccaactcc
    11161 tcagcctaag ggagtatctc gagaagaatg tgaacaatat tataaagaag tgattgctca
    11221 ccttcataaa ttaaaaatgc atagtgcttt agaaaataaa gctcaacgta ttgctgagtt
    11281 ttgtcattca tctgcatcag gcatttttga tttaccctat ccaccatctc aagaattagc
    11341 cctggaaata gctcataaca taattgatag ttaccgagac gatgaaaatt taattaacga
    11401 ttctttctta aagttggaga acaacgaacg agctcaaatc ttatcagcat taattaaact
    11461 gagcttatca caaatcgaag taagtgagtc tcaaaaaact cccgttaatt caaaatttta
    11521 ttctaccatt aaacaatggg aacgattgct tattcccgta gatgaggcca tatcaagaaa
    11581 aatctccatg ttggcacctg ggggtactga acctacaaat atggatctac taagagaagt
    11641 ggacgttgcc ctacatcaat ctccagttag tcttgaggga ttatatgaag gacaaaaagg
    11701 attacaagca gctattacca cctatggtat ggaaaaaata gttgagggcg ctgatgcgaa
    11761 tctgcgtaaa ctggctgata gattgggtat gcgtaacggg gaagctttgt tagcctctca
    11821 atttattaac tattattcaa atactaaact tcttttatca agtgatggga taaatagtac
    11881 tcagggacga gagttatttt ccagggcttt attagatgca tttcaaaatg caacacctag
    11941 tgagaaacag caacttttag cgtttattca aaagttagag tttaatgatg aagtagaacc
    12001 ctgcacatta aaaacatctc accaagcgtt tatagatgaa gctttgttga gatgctctaa
    12061 tcttgatcca gaggcattta gagaatattc tgttgcaaaa gtagaggaac attcccctga
    12121 gacgcttctt tcatttattg acaccttaac gaaagaatct aatgaggatg gaactgacaa
    12181 actatttgga tttgcaaaag aaatcaatat tctttcatta caaattgagc acgcattaaa
    12241 tagtaaacct gtagatgctt cacgtttaga tgtgctttac tctaaagtta tatgctccaa
    12301 tctagcatat caattactcc tggatcaggt aacggaagaa actgctgcta atcttggtga
    12361 aaactttgaa tacactcgag aaatggctct agcccaagca aatattaata aattacagga
    12421 taatctcatt aattttgcag gtaacctgga tcgtgagtca cagtttttta gtgttttcag
    12481 taaatatgtt aatgagaaaa aattcgatgg cccccctatc cttgcgcaat ctgcctcacg
    12541 atccgatata cctggattta tttctttagg aggtaataga agtcttgatg tacttcatgg
    12601 tgccgtatat gttggaaata atcgattagg gatgatgcct gcgcatttac aatcacatat
    12661 cgcagtaaat gaattaggac ttaatcagct tcctttcagg cctttgggcg gtggatatgc
    12721 ttattctgaa ggtaaagaaa ttaaagcatt tataatgcct tcagaagatg gaacggtgac
    12781 aattcagcga gaattaatga cctttgatgg tacttctgaa atgcttcagt atatttctcc
    12841 cgagaagatg gattcagttc caatggcatt aaagcgccga ttgaatgcgg agcatttctt
    12901 tattgactct aaagggacta tacatgcata tactgctaat tttaagccta ttttaaaatt
    12961 agcaaagcat gaagagttat ggactggaga attgctagat cacaatggca ataaaactac
    13021 tataagtctt gatagcgaag gaaacacacc cataattcaa aatttgtcgc gggtatttcc
    13081 acagggagaa atgattagtg ttgattcaaa tacagtgtac ataccttcaa tttcgaaata
    13141 tgttatttct gataaatcag gcagtaatta ttttattagc gacaatttaa tcgatagcac
    13201 ttcaagaaaa catttaacca ttatggatca aggaagtgcc catactgaaa gagtattaac
    13261 tgaatctgag tctcaagagg ttctttcatt acaaaagcaa attgataaac tcaaagaaga
    13321 acatgctttg attaccaaat cagatttagt aagtaaacaa aacaaaagta aactcgaaaa
    13381 gaaaatcaag gaatgtgagt ctcgtattcg agatattacc actccggaaa actttgtttt
    13441 tgtttctgat tcaaaacaaa ttcaatctct tgaacaagaa catttaagat taagaaagga
    13501 aatgcaacaa gcttatactg atttccgaga aggaggaaag aaggataaag aaaaactgtc
    13561 aacgtactat gaaaaagcta aagaagatta tttacaaaac aagaaagcac tgcaaaaagc
    13621 tgactctcaa gcaaattatc taagagtctt tgatgtcaaa gaaggaactt tacatgccaa
    13681 agatttcgcg agtattcttt atgtaggctt gattcctggt aaaacagatg tgctaactca
    13741 atcattagca tctagtgtac caaagtttcc tttgaagctt aacgaactag aagacttaag
    13801 aaatcttaaa gttcaatata aagataaatc acccatatcc aaagaagagc aattggcttt
    13861 aattatgctc ataggtacgg agttacagca tcatatatta gagcgaagtg cggctgcaac
    13921 tggtaaatta agagattggg acagagctac gtacaataaa ttgttaacgg aatttactga
    13981 agaggtaaaa cgagctaatg agctccatgg caaaccccca ctatcccaat ttggagagtt
    14041 atggcgggca atacaatctg agttttcttc tgataaagaa ttacaagaaa tatttaccaa
    14101 atctgtgcag ttggtggata caggtgagaa aaagcctata aacatcaaca ccaaaactac
    14161 aaatatgcct attgaaacta tgggggctcg taccttagtt gaatttaaac tccatgataa
    14221 tccccagtca cttatcgatc cggaacaagt agaacttgaa cgaagtttac agggtataac
    14281 tggattcgaa tcgtctgttc aagctcaaga agatggttat tattatgaaa attatgggct
    14341 atttaacacg aatacatttg aaaagttgtt tagtgttacc taccagcatg ctggtattgg
    14401 tggattaaca aaagaacaag ttactgattt gtttaagtta atggaaaaag aaggatggat
    14461 acgtaaagtt ccaggaatgg aggataagaa taaataccaa ataacacgcc atccaagtga
    14521 gttttattct tcagctaaaa tagcaggtta tcttaatgag ctgggattcg aacggaatca
    14581 gtccaatgca atttctgaaa gattggaaat ctttttgtac cagaccgctg ttaatggtgg
    14641 tagttataag gtaaacgaaa agaatcgaga ggaactgatt caaaaagtta ccgaagctca
    14701 agataaatgc aacttagaat atttagaagc attggataaa attgaatcga cactggcaaa
    14761 ggcttcttca aaaattactt ttgccgattt aaatgcagca tatctcttaa atgattacag
    14821 cagtattcta gctgatttcc cagagaaaga tagagcacaa gttgagattg ttttgaataa
    14881 tggtatgact cgcttgctgt attataaaac tgagttagat catttaaatg atgttcaatt
    14941 taaattcaaa attggacaag attcaacagc agttgctatg ttgcatacgc gcagaaatta
    15001 tcaattagat aaacttcttg attctgaaac tactctggat gataaatcaa ctaaagaaga
    15061 gattcaaaaa gtttcggaag agcgaaaaat gcaacgagca tttctacttt ttgaatctga
    15121 atttggacac cgatgtaatg ctcgtcaagt gaacattttc cgtggattat tactggatga
    15181 tgccacagat cctgataaaa tagattcagc tcaagcaagg atgggatttg gtaaaacctc
    15241 tttattacct ttggttgcat tgtataaaac tggcgataag ctagttcggt tcatagttcc
    15301 caaatcggca cttgaaacca atacagcaga tatgtctaca acccttacaa atattcttgg
    15361 tcgccgtgcg gttaaagatg attttcaacg ttatcgaatt gcaactgatc ctgaaacggg
    15421 tatgggagag aatagtccat gcctcaaatc attacaggac gctaaggcag acttgcagaa
    15481 acgactcgct ctctaccata gagtaagaga taataaagag gttctggttc aagctcctag
    15541 cgtacgtaat tcaatggagt gtcaggcaaa aatatttcta gacatgttaa tgaaggtaac
    15601 ggatgagcct ttacagaaaa aagagctaat ggcatgtatc tctttattaa atgaaattcg
    15661 atctttgact actgtttccg tatttgatga acttgatgcg actcaagatc ctgctacaac
    15721 agaagttaat tacactgcag gggagaaaat ttctcttgat actgcagaaa tttatccttt
    15781 ggaagtgatt actcaaacaa tcggtgcaac agaagataag tctatcgaaa atcttgcaaa
    15841 agtattactt aataaatttg gtattgaaga tgtcgatgag agtattttta aatacatcac
    15901 ttctttaaaa gaaaaacaac ctccttctgt cactccttct aacagcacag aaatttattt
    15961 aattcgcgct attctttctg atccagttat gctaagcatt tttactgaaa aagagccagg
    16021 taccgatttt ggcgtgtggt ttgaaaatgc aaaagacgga tctaaattat atgattacga
    16081 agctttaaaa acaggtaggg acgattcaag aacccctcta ttaattgcaa ttccttattc
    16141 tgcagcaaat acaccaaaac cccaaggttc aagatttgat aatccagaag ttaccgcgat
    16201 tacaactatg ttatattatc ttgatcctcg cacagagatc aatgaattgc cccatttgga
    16261 atttttaatt gattctatga gaaaaggtat cggggaagca cctttcttag actcctccgg
    16321 tgagcatcta gaacctgaat ttaaaaaact cttcaacgaa attagtgcat tagcagaaat
    16381 tgaagatccc ttgattcgta atgaagagcg caaaaagtat ttcgcaggtt tagaggagcg
    16441 aattaaagaa ggagagatag ctccaacggc acttcgtaaa atgttggctc gtaccatcat
    16501 tcaagagcaa gtaaaatttg atgctggcaa ggcgaatagt aatcgttatg aacaaggcac
    16561 taccgatgac gttgtgatcg gattttcagg aaccgctggt gatacttctt ctcactttaa
    16621 agaaaatatg ttggatcctg ccgctgatgg caacatgact ttgggaatta tgggtaggaa
    16681 aaattgccaa gctactacgg ccttggatac gacgagtttt gcagaaaaag gagaggatta
    16741 caatactgtt ttagtcaaac agttagctgc ttcctttacc tccaataccc gaacactgat
    16801 tgatgtgggt ggtttatgca aagcatctaa tcgtgctgta gcaaaagaga ttgcattgca
    16861 gttaaaagaa agttcagaca gcacacttaa gaatcttaaa ggagtaattt tttatgatga
    16921 tgtaaccaat atgaaaaagc ttcttgttct tgaggagtct ggaagagaaa aaattgttga
    16981 tttaacttca gatatggttg ctgagtctga ccaaaaaggt agttatttca cctattatga
    17041 tcaaagccac tcgcgaggtg ctgatataaa gcagatggac aaggcacatg cggtgttaac
    17101 aatgaatttc aacgtaacga ataatgacta caaacaagcg attatgagga tgagaaaaat
    17161 cgttgataag tcattaggac agtctttctc gacagcagtt cctgataatg ttcgtgaaaa
    17221 gattattgat gaacttaatc ttggtaaaga acacacactt acaggtaatg atattgctgt
    17281 ttggttaaga caaaaagaat tgaaaaataa tttaaacaat atttcagtat tcacgatgga
    17341 gttagattcc atcattaaaa atgccatttt gcaacaacaa ggaaaaatta ctcaattaat
    17401 gtctcagtct ccaatgaccg aagaacaact cgaggcattt cgtgaatgta ttcgggaatt
    17461 aaataatatt tcacctttca tctctggtgg cagtacggat ttaattgaga aatacggtaa
    17521 agtttatgga actgtaaaaa aagaagattt tgtcaaagac ttgcacgaat catttgggaa
    17581 gcgattaaat catgtttttg atatagttaa tcgtgctaga gaaaatatta aacttcctct
    17641 tactacagaa agcgatagag ccccctatgt cgaaatggaa aaaaggattg tccaaaaacg
    17701 cgaggctcaa ttaagtccgg agtttgttat tccttcttct tctagtgctt tggctgaggc
    17761 acaatctgaa actgaaaatc aatccgaaag tcaatcacaa agccagagtc aaagtcaaac
    17821 gcagacacat tctttttctg aggtggccaa tgaggaggtt gtagttgagg caaaattaag
    17881 aaagcatgaa gtgcttgatg agcccgcatc aatcgcgtat ttatctgaaa gtgaaaaaat
    17941 tgacaaatta gttttagcaa gtcaaacacc tcacatgcag catttattta aagatgaaga
    18001 tcctattcga tgtagtcctg cctattcggt gtcaggagaa aaagatcata ttttacctcc
    18061 tgtacgttat tttattgctc gagaagaagg taatccgaag ataatcttaa ttaaccagga
    18121 tgaggctaat ttatttaaat catctccagt agatccatgg agtctttatg atgtccgatt
    18181 aaaaaaagga gaatcgcttg agccaatggt aggttcctct attgcatcat taaacgagtc
    18241 aattttgaaa aaagtcagtc ttgcctcatt ccgttatgaa ctgaaaggac aggatatcga
    18301 atctttagca caatctctag agggtatttg tacgccgaat caacttcatc cttcattaag
    18361 tatgaaatat gaaacaagca aaaatatagc taaagatggt ggtgttttca gcttgcctaa
    18421 atggggtttt tatggagaaa aacagcaaga tattcagttt gcaattgagc agactcaagt
    18481 aaaattcaaa gaaggaaaat atgaaaaaag aggggttact gtctcatttg gtaaaggaga
    18541 tgaaagggct gaagtgttta tttcttctaa attaaataaa cgtatactgg atgaagtggg
    18601 caaacaagaa cgagcagaag tccctgtcgg acatcctaaa cttaaggctg taatagaaca
    18661 agttaaaaaa gaacatgatt cagcaatgaa ggagcgatca gaacttcgat ctgatttaaa
    18721 agatctaaaa tcaagaaaaa ataaagtaat tcaagacgca gaccaaaaaa tcagcgagct
    18781 tgaggagaaa aaaagaaaag ctattgaaca agccagagtg gatatttcta aaggctttga
    18841 agatggaatg aaaaatcaaa taaatcgcag aaaatacttt caatcttatt ttgctaaaaa
    18901 tattggtgca atgtgtagat tgagagtagg cgataataaa gattttgggg ttcaagtttt
    18961 tggaaaaaca tttcctaatt tagaaaaggc aatcgcacat gctgtaactc aactttatgc
    19021 agaacataaa aagaaagcta tgacttctga agagttaaac agcagagtag atttttatat
    19081 tgaagagata gtgaaagcag cagaagagcg atttcactca gtaggacgtt atagagataa
    19141 aacgacaaca gctttggaaa ctttacatgc tgctagtaaa gaagcaaccc ctatgtccaa
    19201 agaaaaaatc gagcttttat taaaggggga gttgcaagat cttaagcaag cgtggatgaa
    19261 aggtgataaa tgggataaat tagttgctgc cctagaggag gttaccaaca taacgggtaa
    19321 aaatttatcc atagatgaat ttcatcaaaa aacccttgaa gccttgcaaa aatttgcatc
    19381 agaaaatgga gaggatcttg ataaaacagg tattgtacct agattatttt cttcattgac
    19441 gctaggagaa aaacctcgaa ataaagatgg atttatggcg ggatttctaa atgaagaccc
    19501 taataaagtt cgaaatcatg cagcaattag aagtgatata gccaacttta ttaaaagagg
    19561 ttttgccgga aatacgaata ctccttcaga ggagcaacgt gagtttttta ccgctgttaa
    19621 ctctttggtt aatagcaaaa taaatcgcca caactatgat gtaccagcaa cactattaga
    19681 ttttgaaagc cctatttctt tagaacgaat gaatgaagga attaagcaaa ccttaacaac
    19741 acaaaacgtt cgtactgtca aaaaagatac ggattcaatg gctagagagt gttctgctta
    19801 tctagttggg cgtaaagcgc ttgaaaatat actggatcat tataaacctt tagaaaatag
    19861 gtcctcgcaa atcgatcccg tagagggtca agaagcaaaa tttttctcag caaactttga
    19921 aagactaaca aagttagata cagaagtgct cgatattcaa agacaaatag atgaagttaa
    19981 gtcacagaaa aaagcccatc taggccaatt agaagaagag catgttgtat tacgagataa
    20041 actgcaaaaa aatggtgaaa aagtatctga gcttaaaggg gaaatatcag cattaaacaa
    20101 attaatctcc ggtattaaaa aagtgttgtc agttttcact aaccatcaag taaaaattga
    20161 gcaagatgat cctatcgatt ttcttgatac tcatttggat ctcggtgcaa ttattcaatc
    20221 cgaagtttct gcaacggcaa ctcttacctt tacacctcct gagttttatg atctagaaaa
    20281 agatatgcaa gcacaatttc taaacatgca tggcttagag gaaacaacag aaagaaggtc
    20341 tgaaaaatca tttgatgaag cagcaacttt agtccgagaa actgctggaa aattccaaga
    20401 aagggagagt ttaactgttg gattaaaact ggcaagtagc aaggaaactg aactggttga
    20461 taaagttcaa ttagaacagg aagctccaaa ggtagaaagg gcttcatcat ttaagcaaca
    20521 attatctcaa ataagaaaag atcaagtaga agcattgata acacctatcg tggagcaaaa
    20581 cccgactcag gcatttaaaa ataagttgaa acacgaaatg aacaaagatg aggaagttgt
    20641 gatgacagta cctgtacatg aaacgactaa ttcatttaaa gataaaataa agaaaataag
    20701 agataatgag acgactgctc ctgatattgc attaacagga cttacgccaa accctaaaga
    20761 ttgagaaata gatcaaagag gaataaataa gagaaagata aaagttggca tggtttaatt
    20821 gattttgagg gtagtgttta aataactgtg tcatctcttt taacttgtta taatttaacg
    20881 cttaaaagag gacagcatga ataataaaaa aatagactta cctaattttg attttaatga
    20941 attcaaatca gaagcattaa cccaattaaa atcaggccaa tcattgacag gcaatgtgtg
    21001 attcatagac atcgtttcaa taagttgata gatttacccg ccttatgaag cagcaaatta
    21061 aagccaggtt aacttgggta aatctatatc ttgaaactaa agatgcagga tatgtttgcc
    21121 ataaatgtgg tatttctcgt cctatcctga gaaagtggta cagacgttat ttagaagcgg
    21181 gtatggatgg attaaatgat cgaagcaaaa agcctcattt gtcaccaagt aagaagttaa
    21241 attcagagct gattaaacta attcttgatt tgcgtataag tcgtaatctg ggcgccagac
    21301 gtatccaaac tgaattgatt agattgcaca actgtccgct ttctttagcg agtatccata
    21361 aagcgttaac tagcaaccaa atacaaccaa taaaaaaaac ttaagcggaa aaagaaattt
    21421 aagcgctata gtcgtcctat tcctggtgag cgtatccaaa tggatacctg caaaatagca
    21481 ccaggaattt atcaatatac tgctgttgat gattgttctc gctggcgtgt actagagatt
    21541 tacaaacgcc gtgcagctac taacacacta cattttttag acatggttct ggagcaattt
    21601 ccttttcctg tacagcgtat tcaaacggat agaggattag aatttttttg ccgaaaaagt
    21661 tcagctaaaa atgatgaaat taggtattaa atttagaccc aataagccag cttcccctca
    21721 tttaaatggc aaggtaaagc gatcgcaaaa aacagacctc gaagaatttt atgctattac
    21781 agatttatct gactttgaaa ctcttagaga agagctagct caatggcaat tcttttataa
    21841 ctggcagcgt cctcatggtt cacttaaagg aaagcctccc tcggctatcg tggctgaatt
    21901 aagcgaaata actcctttaa gcgaagaggt gagtaataac tataaaatag acaatgagcg
    21961 tattcaaata gctaattatc atactgattt gattatgaaa aaattgaaag gatctctatg
    22021 aatctgatcc ctatcttttg agaacttatt taaaatatgt attttgttat tttttatagc
    22081 ttggcacctt cttggtattt ttccaaatat catatcgctg attggatatt ttttttacat
    22141 ttaatgcttt cttaatacta cacttctata ataaacatac aatttgaaac atttatattc
    22201 atcaatgtgc gtattcgcac gttttagaat atcagtttgg gagaaatcat aatgcctaag
    22261 tatgtcgaag gggtagaact cactcaagaa ggtatgcatg ctatttttgc acgcatgggg
    22321 tatggcgata ttaccagcgg cagcatttac aatggagtgc caaccattga cacaggtgcc
    22381 ctgaacaggc aaggtttcat gcccgtgtta accggggtag gaccacacag agactccggg
    22441 cactggatta tgttaatcaa gggacctggc aaccaatact acctctttga tcctttaggc
    22501 aaaacatcag gtgaaggcta tcaaaatatt ttagcagcac aactacccat gggttctacc
    22561 ctttctgtta ttcctaatgg ttctaattta aataggggat tatgcggcta ttgggtcgcc
    22621 tctgccggtt taagagcaca gcaagcatta aatcaacata atccccctac tttgttgaat
    22681 ctgggccaaa ccattaccga tgaaatgcgt aacgaactcg atcatgacgg ttatcgaaaa
    22741 attaccggat ggttacgagc tgttgctgac gaattccctg agggtgaccc acaattagat
    22801 gggaaagcat taagagaaaa cactgaaaaa gatttaaaaa tagaaattcc taccctgatt
    22861 cttccgggaa aagatacctc tcctaaagaa acccctacaa cacctaccgc gccacaagta
    22921 gctcccaaat attctttgga cagtaagtta ttagagaatg atgatgatgt tctcgaaacc
    22981 ataaactatg tgcataaaga gtatttaaac aaagactatc ctggtccatt aaaaaaccca
    23041 aaggatccaa aagaaggacg catccctcca gatgagcaga gcaggataaa tcatggtctt
    23101 gcccatacgg tacgcacgat ggcctgtgct gaagtgatga tagaagaagc acgtaaagcc
    23161 aaacttagag gagaaacttt aggtaaagca aaaaatggcc agactttagc tgatgtaaca
    23221 ccggaagaaa tgaaaaaaat tctcatcgca caagcctttt ttgtggttgg aagagacgat
    23281 gaacgctcag ggtatgatga atttcacaaa agaaatttct acgcggaata tcatgaaaaa
    23341 agtgagcaag cgttcaggaa atacgttgaa gacaatcaac tcattggcaa aatctttaaa
    23401 gatcaaaaag aagttgactt ctatgccgcg attattctgg ataaagatca tgattggact
    23461 gccactccag cacatatcct gataaatcaa ggccatatgg ttgatttaat gagagtcaaa
    23521 actcctagcg aagtgacatt agaacgagct tttaatgcac ttaaaaatac cgtcggttcg
    23581 aaaggagccg aggctgttct taaagcccac cgagatttct tctttgctac tggcgccgtt
    23641 gttcctttag taaaccctga agcaatcgat gaccccagta gaggcggacc ttatgaaaat
    23701 ccctacaacg gagaaaaata cgttattgta gaaggcaagg aaccaaaatc tacgaaagac
    23761 ttacccaaac ctgtaggtcg taactatcaa ctgaaagata atgaacgatt cctaaccata
    23821 aaggaatatt acgcttttcc tgacgtacag caaatctatc ccggttataa aacacgtttg
    23881 gaaggaacct cttattattt accaacacca tttgcaagag aatgtgagcg agacccggca
    23941 aaatgcctgg gggctatcca aaaagcccga tcaaaactac aaacggatgc cattaaaaat
    24001 ggctttcaat ccagttctga taaagcgaga agacaaccta atgtggatga aattgctgct
    24061 gcccgcatca ttcagcaaat tttggccaat cctgattgca tccatgatga tcatgtgttc
    24121 ctcaatggcc agaaactgga agaaaaattc ttccgcgatt tgctagcgaa atgtgaaatg
    24181 gctgttgtag gctcactgct taatgacacc gacataggga acatcgatac tttaatgcga
    24241 catgagagag acaccgagtt tcacgctact gattctgatg cagttccgaa gaaaattgga
    24301 gaatattggg tcaatgacca aaataaaaat aataaaagta acaacatcac tcaaaagaaa
    24361 cacgatttaa ttttcctcat gcaaaatgat aagtggtact ttagccgagt caatgccatc
    24421 gcacaaaaca gggataaagg ttccactttc aaggaagtgc tgattactac tttaatgacg
    24481 cctttgacca gtaaagcatt agtcgatacc tcaagtgccc ctgctccgaa aaggctgttt
    24541 cgtggattaa atctctcaga agagtttacc aataaattaa taaatcaaac caatgccatc
    24601 attgccaata cagaaaatac gctttttact gatctctctg cagaaacctt taaacaaatc
    24661 aaattaaatg acttgagcca aatgtctggc agaactaatg ccagtacaac cacgaatatc
    24721 gaactgtgta gaacaatctg ggattctaat gtcatctttg aaatgcttga cccggatggt
    24781 ttgctgcatc ccaagcaagt aggacgccat ggagcgggca ctgaaagtga attctccgtt
    24841 tatttgccgg aggatgttgc cctggtccca gtcaaagtga ctcttgattc aggtaaaacg
    24901 caaaatggag aaaatcg
//
LOCUS       DATNSN010000032        24256 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.032, whole genome shotgun sequence.
ACCESSION   DATNSN010000032 DATNSN010000000
VERSION     DATNSN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24256
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.032"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(10..660)
                     /locus_tag="VAH76_001071"
     CDS             complement(10..660)
                     /locus_tag="VAH76_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEO1385717.1"
                     /translation="MTYSFSKPVSWVFLFTAVIYLVSLSFIQYPVTTVLKPIPIVCLI
                     VGVFRTFLPLLAKIFLILALVFSLAGDVVLTLPVALQLELGIGCFLLAHCFYIALFLK
                     SFQFNKLHFFYYILILLLMGFIAFTIIPYLGDLLIPVMIYFCVLMLMVFSAFQVKKET
                     LTIGSGALFFLISDLTLALNLFVYTQADVRVFVMFTYYVAQFLLIFGLVRIYKEEF"
     gene            complement(740..1240)
                     /locus_tag="VAH76_001072"
     CDS             complement(740..1240)
                     /locus_tag="VAH76_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385718.1"
                     /translation="MTKEKSESGNSPSNSEEHGTLGPDVYENARKLNIQLATRGPAIL
                     YALGKASEQVTNGNVVTAVGGMVVGAIGFFGNRFYRNKGDDSAYTYMGQIVGAVQFTA
                     GFTLLALQAFEAFTDTGETSPLLNVVKLSGVALTLTESALILINSYLYGDGSAKTTSI
                     TSPKID"
     gene            1732..2592
                     /locus_tag="VAH76_001073"
     CDS             1732..2592
                     /locus_tag="VAH76_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO1385719.1"
                     /translation="MLKRLLGVASLFLILNHQCFAEDHEIAITIDDLPFVGSGTSTPG
                     NLKRTQERFMAIVNTLVDNQVPATGFAIGGAIAKNEWELLEIFRNQGFSIGNHTYKHR
                     SLNSMTAENYIADIEKADTVLSPVMTEPKYFRYPYLAEGSGEKKQKVHEWLAAHQYTI
                     APVTIDSKDYEFNAQFYRIPYRQRPQRLAQFKKRYLAFIWQQTLRAEKKVKKVEGQPV
                     KHILLIHANLINSLCLADIIEMYRSNGYKFITLQEALKGNTATPINDSSPTEALKSET
                     EKKEVNEPQS"
     gene            complement(2710..3906)
                     /locus_tag="VAH76_001074"
     CDS             complement(2710..3906)
                     /locus_tag="VAH76_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEO1385720.1"
                     /translation="MKSKLIYLTVALAAIILSAAVWWWLQSPKPTFRQVSFKHLPGWE
                     NAQLKKSLETFQTSCRAFLKQNPERVVGTEQIDLQAKDWHPACRAALKINPVDEQSAK
                     LFFEKWFAPVEFYDDKPVKGLFTGYYMPLIKGSYNKSDEFSVPIYELPSNLVTVDLGL
                     FLPNLKNKKIIGRIAGNKVVPFYTREEINKGAIKNTARVLVWIHSPVDRLFLEIQGSG
                     IIELEDGKRIFIGYDGQNGAPYTAIAGVLIKKGIMTKHNASMQRIKKYLEAHPKQMDK
                     IINQNKSFVFFRKLSLEAALGSQGVALTPGYSLAIDKQWIPMGAPLWLNTTRPDSKNP
                     DVNKPMQRLMIAQDTGGAIRGKVRGDVFWGGGDKATLIAGHMKNEGHYWLLLPAHALS
                     RLAQLK"
     gene            complement(4016..4573)
                     /locus_tag="VAH76_001075"
     CDS             complement(4016..4573)
                     /locus_tag="VAH76_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385721.1"
                     /translation="MKKTDSGFILLMTLCITFLMSLLVLASLQQIFLQYKALNSQETF
                     HQNFYQLEHWMMRLARSPLLYAGMDCYVQKDYGANKIANELVKNKGCLLILNQKKYRY
                     FIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDDEHGEFSIMQLRFIKLGSNLSSCPEE
                     EIQVKEGVSSWRYLPDYKNFEILTG"
     gene            complement(4570..5376)
                     /locus_tag="VAH76_001076"
     CDS             complement(4570..5376)
                     /locus_tag="VAH76_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO1385722.1"
                     /translation="MLRGLKKANQLFAPMIWVLIEMGRQTGFSLSEMLISLFLASIIT
                     IPLTQFYIRSKPQYLKAQERLEINFDLLWIGDLLSDSIRKAGFTPCVSVEKLKTFDSR
                     HAERKIINFQIENMPQVQLRINRMSDVYTEVANFMSPTQIVIEESVISNIKHPLIISD
                     CRQGEVHTVSKIDKLSNGYQITLKKPLQFRYDEIAYLGEWLEETWFTKQNEKGEKTLH
                     YKLFQTEELSPFIHEFYVTRRLIKQRPVLDFSIGSDKETLRKISVSVRNL"
     gene            complement(5315..5629)
                     /locus_tag="VAH76_001077"
     CDS             complement(5315..5629)
                     /locus_tag="VAH76_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO1385723.1"
                     /translation="MSKHQGFTLMEVLVSLMLMTTVVLTLVEQQLKTRDLINHLIWRI
                     QGSQYLDQVDEALYISENRLPLPPANYHFDIRRSQKKIILNVTWFKKSQSIIRSYDLG
                     AN"
     gene            5789..6898
                     /locus_tag="VAH76_001078"
     CDS             5789..6898
                     /locus_tag="VAH76_001078"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEO1385724.1"
                     /translation="MSIDFQQLPHAGIRSLIPYVPGKSIEELAKEKGITDIIKLASNE
                     NPLGCSPLALSVIQTMSSHYIATYPSPWNHPLMSKLASYLKVKPEQLFLSNGSDYLFN
                     ILLNCFALHTDRHILTHDYAFSTYAIQANSLQIPINSVPIGHNWEVNITDIVNACNEQ
                     TGIIFIANPNNPTGVLIQQEEIKYLLEQIPKSTLLVLDEAYYEFAASQLTVNSLDWLE
                     EHPNLVVTRTFSKIYGMAGLRLGYAIANPSIINILKRVQLPFIVNQVALAAAYAAIDD
                     DDFIQSSLKMNNEGMSQLQAGFNELNIKYLPSSCNFLTFDCEEDSMALYNYLLDNGII
                     VRPLHAYKMNNFIRVTIGTKEQNSRFLTALKNFYL"
     gene            6935..7276
                     /locus_tag="VAH76_001079"
     CDS             6935..7276
                     /locus_tag="VAH76_001079"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEO1385725.1"
                     /translation="MTSDLSSKHCESCEGIGAALNSEQIKNLLPQLNTKWEVTEDNRI
                     IKRAFSFKNFYETMAFVNAIAWIANIENHHPDLEVGYNYCRVHFMTHALNGLTHNDFI
                     CAAKIDKLLVD"
     gene            complement(7454..8371)
                     /gene="secF"
                     /locus_tag="VAH76_001080"
     CDS             complement(7454..8371)
                     /gene="secF"
                     /locus_tag="VAH76_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEO1385726.1"
                     /translation="MEFFNPNSKIDFMGARRWTALFSILIFVVSIGALMINGLKWGLD
                     FTGGTQIEISYSKAADLPLIRENLNKAGFKEAQVVSYGTSKDVLISIAPRADKQQSML
                     VDQVMEVLPGAVKQRVDFVGPQVGQELATKGALAIIVSLLATMIYIAMRFEYKLAVSS
                     AVALIHDPVLILGVFALFGIEFDLKALAGLLAVIGYSLNDTIVVFDRVRENFVKIRRT
                     SPIDIMNISINQTLSRTIMTSVLTLFVVVALFVYGGEAIYGFSLALIIGIVIGTYSSI
                     YVAGALAVAMGLDNKDFLPSHRREVDDRP"
     gene            complement(8384..10240)
                     /gene="secD"
                     /locus_tag="VAH76_001081"
     CDS             complement(8384..10240)
                     /gene="secD"
                     /locus_tag="VAH76_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEO1385727.1"
                     /translation="MQNKYPLWKNLMLIVIAVIGFIYAIPNLYTENPVVQISAGTDED
                     TETIKNQVESILKNANMDYKSLVVERDGVEVVFSSTDSQLLARDVIKNSLGSDYTVAL
                     NLAPSTPAWLEAIHADPMKQGLDLRGGVHFLLEVDVESVISRRYEGIMKNIGQELREA
                     GIRYSGIRYLSDKGIELRFRDSDSMENALVELRQKISDVVFVKTKTQNSILGAISPAE
                     LNSIRQNTIEQTMSILRNRVNELGVGEAVVQQQGATRVAVDLPGIQDAARAKQILGGT
                     ATLEFHLVDQENDPFIAKQTGVVPVNDKLYMMDGHPILLKRQVVLSGDSITSAVSSFD
                     QQTASPSVQIQLGGGGESLFTKVTRENIGKRMAIVFVETKTTTQTINGEEKRVTKKEE
                     RVISAPVIQNALGSNFQITGLTDSKEASNLALLLRAGALPAAIYPVEERTVGPTLGKE
                     NIRRGLISLEVGMGLILVLMLVYYHFFGLIADIALMLNLVFLCALLSIIDATLTLPGI
                     AGIVLTVGMAVDANVLIYERIREELRNGLSPQAAIHAGYERAFSTILDANVTTLIVAI
                     ILFAVGTGPVRGFAVVLSLGLLTSMLTGITYTRAIVNWYYGGRNVKKLSIGI"
     gene            complement(10262..10597)
                     /gene="yajC"
                     /locus_tag="VAH76_001082"
     CDS             complement(10262..10597)
                     /gene="yajC"
                     /locus_tag="VAH76_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO1385728.1"
                     /translation="MSFFISDALAAAGNAPAAQTDGTFSLIMIVAIFVLFYFMIIRPQ
                     SKRAKEHRELINRLKKGDEIVTSGGILAKVVSLDDQYIKVSPAEGIEINLQKGAVTAV
                     LPKGTIKSL"
     gene            complement(10743..11576)
                     /locus_tag="VAH76_001083"
     CDS             complement(10743..11576)
                     /locus_tag="VAH76_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuine tRNA-ribosyltransferase"
                     /protein_id="HEO1385729.1"
                     /translation="MLSTNQNFVPVLNTEAGLCLTAANWQEIKITIASCYLDLLLLKP
                     GYSLLKNITDFTKYLGWSGHLILNASRLVSDKNGMVVLISPYDGSRIKLTNSELVHLI
                     LHIKPVAVLLPETLVNGFSGLWEQWDDTIFPFLSAKQLETLQVPGKHGVYFNFSEAKY
                     HDDFLSQLQKWSELPLYVSGPIGADLIEDLNRKKSILIESDEPAKNAMRGIVYGREGN
                     VDLTDESQAFNFQLIDENCSCPTCSAQLTRAYLHHLLANTPLLCQRFLIQHNAYYVQG
                     N"
     gene            complement(11623..12636)
                     /gene="queA"
                     /locus_tag="VAH76_001084"
     CDS             complement(11623..12636)
                     /gene="queA"
                     /locus_tag="VAH76_001084"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO1385730.1"
                     /translation="MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFR
                     EIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLK
                     SNDLIYLDAGKRLEVLERQDDLFLCKASENILDLLNDLGHIPLPPYIAREDESLDKER
                     YQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQD
                     HKMHSEWFTVSPDLCSAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIF
                     IYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDAS
                     LLL"
     gene            complement(12751..13164)
                     /locus_tag="VAH76_001085"
     CDS             complement(12751..13164)
                     /locus_tag="VAH76_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385731.1"
                     /translation="MLLTLALVILFATIMVFFSQEFIRTFKKILAIKGAKLLVPLGIA
                     SWLIYNFDYLFIWVIYYIREVLQAVLAFLTRIMPFKPYSTSIALIILLTTVSVGPVLL
                     LDLIYRKRTYKGYAYPYLTSTLILIFCTLILLVVS"
     gene            complement(13246..15099)
                     /locus_tag="VAH76_001086"
     CDS             complement(13246..15099)
                     /locus_tag="VAH76_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO1385732.1"
                     /translation="MSIVSLNGASLILAGNCILDRADLQIQSQDRIALVGRNGAGKST
                     LLKILQGELVLDSGQIQRSSGLRISGLVQEVPGAEGESVYHFLVKSLGETGEVLSQFH
                     EFTKQGDMDKLAKCQQRMDNLNAWHLLPEIETMASRLGIDIHEQMSNLSGGMKRRVLL
                     GAALLAKPDLLLLDEPTNHLDVEAIEWLENYLKSFSGAVMVVTHDREFLSQIATSILE
                     IDRGKLYLHHCNYETYLDRRESIRLSEQKQNDLFDKRLAEEEAWIRTGIKARRTRNEG
                     RVRALKAMREQYKARRNQLGTVKSLNLDVSRSGALVVEANKVSYAINDKPIIKDFSLL
                     MTRGDKLGIIGPNGCGKTTLVRLLLGEIKPDAGTIKLGTGLQVAYFDQLRRHLLEDQT
                     VMFNVGEGADYVTINGKQKHVASYLKEFLFSSDRFNQPVSSLSGGERNRLLLAKLFAK
                     PVNLLVMDEPTNDLDIETLELLEAVLADYPGTLILISHDRAFINQVVTSVLVYEGNGK
                     FNEFVGGYDDYKKHQLTRSEQQTKSPVSKRSSPEKAKNLSFNEQRELSKLPQQIEAVE
                     KKIEALHSQMASPEFYQQDAKIISEVTQLLAKEEALLNQYFTRWEELEEKK"
     gene            complement(15160..15651)
                     /locus_tag="VAH76_001087"
     CDS             complement(15160..15651)
                     /locus_tag="VAH76_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEO1385733.1"
                     /translation="MSDDQYARTGRLMFLFVWIIFFIGMFLFFYYYDKPESTIFVASR
                     TEYVLSADNEGHYWIKGKINEYPVEFLVDTGATLVAIPQGLAKDLKITGRYPVTIETA
                     NGKVTGFLTRLQHLSFGEFHLQNVKAVIIPENDDNTVLLGMNVLSQFHMIQQGKQLIL
                     KRQ"
     gene            complement(15654..16040)
                     /locus_tag="VAH76_001088"
     CDS             complement(15654..16040)
                     /locus_tag="VAH76_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEO1385734.1"
                     /translation="MQPINTKLAPKAIGTYSQAIRSGEIVFLSGQIPLDPETMQICSD
                     DIKLQITQVLENLSAVCEEAGGSLANIAKLNVYLTDLSHFPLINEAMSRYFSEPYPAR
                     AAIEVSALPKGAKVEMDGILVLPASA"
     gene            complement(16040..18163)
                     /gene="spoT"
                     /locus_tag="VAH76_001089"
     CDS             complement(16040..18163)
                     /gene="spoT"
                     /locus_tag="VAH76_001089"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO1385735.1"
                     /translation="MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEP
                     YITHPVAAALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTK
                     LTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
                     ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEK
                     DLQHALSQLNIPYEQVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYR
                     ILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADN
                     GVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
                     TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAP
                     GAHPNPAWLNFVVTGKARSNIRHFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPE
                     TLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTE
                     GMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQ
                     GEYWVDITVDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTH
                     LARVMRRLRANKVVMRLYRKKQGEI"
     gene            complement(18331..18534)
                     /gene="rpoZ"
                     /locus_tag="VAH76_001090"
     CDS             complement(18331..18534)
                     /gene="rpoZ"
                     /locus_tag="VAH76_001090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO1385736.1"
                     /translation="MARVTVEDCLEHVKNRFELVMVATKRARQIAVRGDQPMVEWEND
                     KPTVVALREIAEGYVTADILDED"
     gene            complement(18585..19214)
                     /gene="gmk"
                     /locus_tag="VAH76_001091"
     CDS             complement(18585..19214)
                     /gene="gmk"
                     /locus_tag="VAH76_001091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO1385737.1"
                     /translation="MASDNSGNLYIVAAPSGGGKTSLVKKLIEMVGEIEVSVSHTTRP
                     MRPGEKEGVDYFFIDEEQFISMVNEGAFIEHAKVFNHWYGTSVVQINKRLQFGIDVVL
                     DIDWQGAEQIRHAYPDAVSVFIIPPSLDALNERLMNRRQDKDHVISERMTKAQDELGH
                     YPEFDYLIVNDDFEKAAMELQSIVIANRLRIEKQVNKQAKLLSFLLSSQ"
     gene            complement(19220..20086)
                     /locus_tag="VAH76_001092"
     CDS             complement(19220..20086)
                     /locus_tag="VAH76_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEO1385738.1"
                     /translation="MIHSMTAFSRIQKQFDVGDFCWEIRSVNHRYLDVSFRLPESFRF
                     LEAELRSLLRDKIHRGKLECHLKYKDTSCDNRSMLINIGTINALLDLSGKLSASHHLA
                     NDMTVSQVLSWPGVVEANPLDMEELSQIALELFQKSIDKLCEVRAGEGRSLKAYVEDR
                     LQALRQEVMKSKSIMVSMSAKTKDKLLTRLHELQMDVTEARVEQEIALLLAKLDVSEE
                     LDRLETHIAEVNRTLNAEKVTGRRLDFLMQELNREANTLSSKSDSAELTLCAVEMKVL
                     IEQMREQIQNFE"
     gene            20387..21094
                     /gene="rph"
                     /locus_tag="VAH76_001093"
     CDS             20387..21094
                     /gene="rph"
                     /locus_tag="VAH76_001093"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEO1385739.1"
                     /translation="MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIV
                     EGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACI
                     DLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPVFNYVA
                     AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSML
                     SLAEIGIPQLIEIQKNA"
     gene            complement(21426..22460)
                     /locus_tag="VAH76_001094"
     CDS             complement(21426..22460)
                     /locus_tag="VAH76_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEO1385740.1"
                     /translation="MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEH
                     KDVIKIIYDIMNDRQRKEYEEFLETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSE
                     ILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSTRYEHILTVED
                     PIEFVHQSKKCMVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTNSATKTINRIIDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGR
                     VAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTA
                     HEAALNKSLF"
     gene            22624..23319
                     /locus_tag="VAH76_001095"
     CDS             22624..23319
                     /locus_tag="VAH76_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO1385741.1"
                     /translation="MSISQNIKKVRELLTQTELSSNRQPNSVLLLAVSKEQSPELIRE
                     AFEQGIRHFGENYFQEAQEKILALKDLPICWHFIGPIQSNKTKGIAKYFNWVHSIDRN
                     KTAKLLNQFRSEQLPPLNVCLQINLAGETTKSGIPPEHAIDLALEVKQLPNLQLRGLM
                     TIPPQQNNMQTQYDLFLKLNQLKESINKTLHLKMDTLSMGMSDDLVPAIKAGSTIVRI
                     GRAIFGERKGKLL"
     gene            23316..24104
                     /locus_tag="VAH76_001096"
     CDS             23316..24104
                     /locus_tag="VAH76_001096"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO1385742.1"
                     /translation="MNISFIGYGNMAKAIACHLQKQNKYSLSAASPSLSAGISKENIR
                     THYDNKEVVKDADVIILAVKPTQMREVVNEINPFISSGCLLISVAAGLSLTWFAKYCK
                     KGQAVVRTMPNTPAIVGFAATSMIANEFVNEKQKKWAEQLFSSIGITDWVKDETDMDT
                     LTALSGSGPAYVFLFLEAMINAGVQLGLEETVAKHFAIQTFSGSLQLAQHSQLSLTEL
                     RKKVTSPGGTTAAALEILHGDLDELIFSAMKAAKQKAHELGKVE"
BASE COUNT         7628 a         5138 c         4128 g         7362 t
ORIGIN      
        1 aataatcccc taaaactctt ctttatatat tctgaccaga ccaaaaatta ataagaattg
       61 agctacataa taggtaaaca taacaaaaac ccttacatca gcttgggtat aaacaaacaa
      121 gttaagtgcc agggttaaat cagaaattaa aaagaacaaa gcgccactcc caatcgttaa
      181 agtttccttt tttacttgga aagcactaaa taccataagc ataagaacac agaagtaaat
      241 cattactgga attaataaat cgccaagata cgggattatg gtaaatgcta taaaccccat
      301 taatagaaga atgagaatat aatagaaaaa atgtaattta ttaaattgaa atgattttaa
      361 aaacaaagcg atataaaaac aatgcgctaa caagaagcaa cctatcccta attctaattg
      421 taatgcaaca ggtaaagtca ataccacatc ccctgccaag gaaaatacaa gtgccaagat
      481 caggaaaatt ttagccaata aggggaggaa agttctgaat accccaacaa ttaaacaaac
      541 tataggaata ggctttaaaa cagtagtaac aggatattgg ataaacgaaa gactgaccaa
      601 ataaatgact gcggtaaaca aaaacaccca agacactggt ttggaaaaag aataagtcat
      661 aaatagtccc ttattgtaaa atcaaagtca ttgatttgag ctgccttatg cctcaaccct
      721 actagccttt ttattgtatt caatcaatct tagggcttgt tattgatgtt gttttggctg
      781 atccgtcccc atataaatac gagttgatta aaattaaagc gctctcggtt aatgttaacg
      841 caaccccgct tagtttaaca acattgagta agggacttgt ctctccagta tctgtaaacg
      901 cctcaaatgc ctgtaatgcc aacagtgtga atccggcagt aaattgcacc gcaccaacaa
      961 tttgtcccat ataagtatac gcggaatcat cacccttgtt tcgataaaaa cgattaccaa
     1021 aaaaacctat cgccccaaca accatgccac ccaccgccgt tacaacattt ccattagtta
     1081 cttgctcact ggctttacct aatgcataca gtatggcagg ccctcttgtt gccaattgaa
     1141 tatttagctt tcttgcattc tcataaacat ccgggcctaa agtaccatgt tcttctgaat
     1201 ttgatggtga atttcctgat tcagattttt cttttgtcat tatcaactcc tgtttaacaa
     1261 aattccgggt aagacagtat cctttcaatt tctcagatta tatccacggc cttgccttat
     1321 tgtttgacat aatcagccct aatttcacgt gagttatgga taagatcaac cgttaccttg
     1381 aattgctcag gctgaggagg catttatgcc gtctcgaagc cttgtatgga atttaatatt
     1441 tcatgcttag gctttgagag gacgctcaga catctccgta gcccaaacgg accctggata
     1501 tcgagtgctc cgtatataac ccttatgcat aactcacgtt aatttaagta attctctatt
     1561 tgtcacttta ataaattatc caatagccga aaaatcgaag tatttattgt acctaaaccc
     1621 tctcaaaaca catacatatt tccaaacagg tggaaattta atttatcgtc tatagttaaa
     1681 ttaaatgcct aaaataataa gaaattttcc aaattgaaaa gaggggaaaa tatgttaaaa
     1741 cgtctactcg gtgtagcatc attattttta atattgaacc accaatgctt cgctgaagat
     1801 catgaaattg ccattactat tgacgattta ccttttgtgg gctctggaac aagtactcca
     1861 ggaaatctga agcgaaccca ggaaagattt atggccatag tgaacacttt ggttgacaat
     1921 caggttcctg ccacaggttt tgccatagga ggggctatag cgaaaaatga atgggagctt
     1981 ctggaaattt ttcgtaatca aggtttttca attggcaatc atacctataa gcatcgaagt
     2041 ttaaattcaa tgactgcaga aaattatatt gctgatatcg aaaaagcgga taccgttcta
     2101 tcccctgtta tgactgagcc aaaatatttc cgttatcctt accttgctga aggcagtgga
     2161 gaaaagaagc aaaaagtgca tgagtggctg gctgcccatc aatacacgat tgctcctgtc
     2221 actatagata gcaaagatta tgaattcaat gcgcaatttt atagaatacc atatagacaa
     2281 agaccacaac gtttagctca atttaaaaaa cgctatcttg cttttatctg gcaacagacc
     2341 ctgagagcag aaaaaaaagt caagaaagta gaaggacaac ctgttaaaca tattttactc
     2401 atccatgcga atctgattaa tagtttgtgt ttggcggata tcattgaaat gtacagaagc
     2461 aacggttata aattcattac tctgcaagag gccctaaaag ggaatacagc tactccaatt
     2521 aatgattcat caccaactga agctttgaaa tcggaaaccg aaaagaaaga agttaatgag
     2581 cctcaatctt aaaaaacata gaggtatttc ttgaaacaaa tgatttacaa tgctatcggg
     2641 gattcattat cacaggactg gtgaatccca atagccaaat aaaaaattgc aaagacagga
     2701 cttacacccc tacttcaact gtgccaagcg cgataatgca tgagccggca gaagaagcca
     2761 ataatgcccc tcgttcttca tatggccagc aatcaaagtg gctttgtcac caccacccca
     2821 aaatacatca cctcttactt taccacgaat agcgccacca gtgtcttgag ctatcatcaa
     2881 acgttgcata ggtttattga cgtcaggatt tttactatct ggtcttgtag tgtttaacca
     2941 tagaggcgct cccattggta tccattgttt atcaatagca agagaataac cgggtgttaa
     3001 tgccacaccc tgtgaaccta aagccgcttc taatgacaac ttgcggaaaa aaacaaatga
     3061 tttattttga ttgattattt tatccatttg cttgggatga gcttccagat attttttaat
     3121 cctttgcatg gaagcattgt gcttggtcat aatacccttt ttaattaata ctccagcaat
     3181 ggcagtatag ggggcaccat tttgaccgtc atagcctata aaaattcgtt tgccatcttc
     3241 gagctcaatg attcctgagc cctgaatttc taaaaataac ctgtccacag ggctatggat
     3301 ccacaccaaa acacgtgctg tgtttttaat tgctcctttg ttaatttcct ctcgagtata
     3361 aaatggtaca actttattac ccgcaattcg tccaataatt ttcttatttt ttaaattagg
     3421 aagaaataaa cccaagtcaa cagtcactaa gttactgggt aattcataaa taggaacact
     3481 aaattcatct gatttgttat aactgccttt aattaaaggc atataatagc ccgtaaataa
     3541 acctttaact ggtttatcat catagaattc cacaggggca aaccacttct caaaaaataa
     3601 ttttgctgac tgttcatcaa caggatttat ttttaaagca gcacgacagg ctggatgcca
     3661 atcttttgcc tgtaaatcaa tctgctctgt gccaacaaca cgttcaggat tttgttttag
     3721 aaaggctcga caagatgttt gaaaagtttc taacgatttt ttaagttgtg cattctccca
     3781 cccaggtaaa tgcttaaagc ttacctgcct gaaagttggc ttgggagatt gcaaccacca
     3841 ccatactgct gcgcttaaga taattgcagc caaggctact gtcagataaa ttaatttcga
     3901 tttcatagga ggtcaatttt tacacgtgat ggagaaaaat gcaactaaaa attattgcta
     3961 ttagtccatc ttactacaaa aaacatccag ttataatagc tttttttcaa taatttcaac
     4021 cagttaatat ttcaaaattt ttataatccg gcaagtatct ccagctgctt accccttcct
     4081 tcacctgtat ttcctcctct ggacaagagg acaaattgct acccaatttg ataaacctta
     4141 attgcatgat agaaaattcg ccatgctcat cgtcttgtaa tacggtgaat ctaaaatggt
     4201 gagaagcata tttttgccca tccttattaa gaaccaaaca agaaaattcg ttcaggtcct
     4261 caataaaata tctgtatttt ttctgattta atatgagcaa acatccttta tttttaacca
     4321 actcgtttgc tattttatta gctccataat ccttttgcac ataacaatcc atacctgcat
     4381 acaataacgg tgaacgagcg agtcgcatca tccaatgttc caactgatag aaattttgat
     4441 ggaatgtctc ctgtgaattg agtgctttgt attgcaagaa tatctgttgg aggcttgcca
     4501 aaaccaataa gctcatcaag aaagttatac aaagcgtcat taaaagaatg aaaccggaat
     4561 cggttttttt cataaatttc gtaccgaaac actgatttta cgtagcgttt ccttatcgct
     4621 accaattgaa aaatccaata ctggcctctg tttgataaga cgtctggtaa cataaaattc
     4681 atgaatgaat ggacttaatt cttctgtttg aaacagtttg taatgtaatg tcttttcacc
     4741 cttttcattt tgtttagtaa accatgtttc ttcaagccac tctcccaagt aagcaatttc
     4801 gtcgtaccgg aactgcagtg gtttttttaa agtaatttga tatccattgc ttagtttgtc
     4861 tatttttgaa actgtatgta cttctccttg ccggcaatca gaaataatta atgggtgttt
     4921 tatatttgat attactgact cttcaatcac tatttgtgtt gggctcataa aattcgccac
     4981 ttcggtataa acatcactca ttctgttaat acgcagttga acttgtggca tattttcaat
     5041 ttgaaaatta attattttcc tctctgcatg tcgagaatca aaagttttta atttttcgac
     5101 acttacgcat ggagtaaatc cagcctttct aatactgtcg cttagtaaat caccaatcca
     5161 cagcaaatca aaattaattt ctaaccgttc ttgtgccttt agatattgag gcttgcttct
     5221 tatataaaac tgagtaaggg gtatcgttat aatacttgcc agaaaaagac taattagcat
     5281 ctcagataaa ctaaatcctg tttgtcttcc catttcaatt agcacccaga tcataggagc
     5341 gaataattga ttggcttttt ttaaaccacg taacattcaa aatgattttc ttttgagatc
     5401 gcctgatatc aaaatgataa tttgcaggcg gcagagggag tctattctca ctaatatata
     5461 atgcttcatc tacttgatct agatattggg agccttggat tcgccaaata agatgattga
     5521 ttagatctct ggttttcaac tgttgttcca ctaacgtcag aactaccgtt gtcattagca
     5581 ttaatgacac aagtacttcc attagagtga aaccctgatg cttgctcata ataaatctct
     5641 tcctgaggtt ggactgccca tgaaggttaa ctgttcgttt atataaattc aatataacga
     5701 tttataaatt ctaaaaaata aattcattcg tgacagctgg catatgctta ggtaatataa
     5761 aaacattttt ctgtctagga atagagttat gtctattgat tttcaacaat tacctcatgc
     5821 cgggatacgt tctttaattc cttatgtacc aggtaaatcc atcgaagaat tggcaaaaga
     5881 aaagggaata actgatatta tcaagctggc tagtaatgaa aacccattgg gatgcagccc
     5941 attagccctt tccgtcattc agactatgtc atcgcattac atagcaacct acccatcgcc
     6001 ctggaaccat cctttaatgt ccaaacttgc aagttactta aaagtaaaac ctgagcaatt
     6061 gtttctaagc aatgggtctg attacctgtt taatatttta ttaaattgtt tcgctcttca
     6121 taccgacaga catatcctta ctcatgatta cgcttttagc acttatgcca tccaagccaa
     6181 ttccctgcaa ataccaataa atagtgtgcc tattggccac aactgggagg ttaatatcac
     6241 tgatatcgtt aatgcttgca atgaacaaac aggaattatt tttatagcta acccaaataa
     6301 tccaactggg gtgctaatcc aacaagagga aattaaatat ttattggaac aaattccgaa
     6361 aagtactttg ttagttttag atgaagccta ttatgaattt gctgctagcc aattgactgt
     6421 caatagtttg gattggcttg aagaacatcc taatttggtt gttactcgta cgttttcaaa
     6481 aatctatggt atggcaggtt tgcgtttagg ttatgctatt gccaacccat ccattattaa
     6541 tattttaaaa cgagtacaat tgccatttat agtcaatcaa gttgctcttg cagcagccta
     6601 tgctgccatc gatgatgatg acttcatcca gtctagcctc aaaatgaata atgaagggat
     6661 gtcgcagcta caggcgggct ttaatgaatt aaacataaaa tacttaccct cttcctgtaa
     6721 tttccttact tttgactgcg aagaggatag tatggctctt tataattatc tattggataa
     6781 tggcataata gtaaggcctc tacatgctta taagatgaat aatttcatca gggttactat
     6841 tggcaccaaa gaacaaaatt ccagatttct aacagcactg aaaaattttt atttgtaact
     6901 ctttttaaat taaaaataaa attaaggaat caaaatgact agtgatttaa gtagtaagca
     6961 ttgtgaatct tgtgagggaa ttggtgctgc gctcaactcc gagcaaatca aaaatttact
     7021 acctcaatta aacacaaaat gggaagtaac agaggacaac cgaattatca agcgtgcttt
     7081 ttcatttaaa aatttttatg aaacgatggc ttttgtcaat gcgattgcct ggatagcaaa
     7141 tattgaaaat catcaccctg atttagaagt cggctacaat tattgccgtg ttcactttat
     7201 gacgcatgca cttaatggtt taactcataa tgattttatt tgcgcagcaa aaatagataa
     7261 gttactggtt gattaaaagc tattgactct ttttaagaag atacatttta acaaaatttt
     7321 gtaattattt attatccgct ccctggcagt cgcggctcgg tttcgtccat aatccgatgt
     7381 ggttcggagt aaactatgat ccgagccgcg accatcaggg agcggataat gcatggacaa
     7441 taagctctcc acctcaaggc ctatcatcca cctccctgcg atgactgggt agaaaatcct
     7501 tgttatccaa acccatggct actgctaaag cgccagcaac ataaattgaa gagtaagtac
     7561 ctattactat tcctataatc aatgccaatg aaaacccata tatcgcctca cccccataga
     7621 caaaaagcgc aaccacaaca aacagcgtca acacagatgt catgatcgtt cttgagagag
     7681 tttgattgat tgaaatattc ataatatcta ttggacttgt tcggcgaatt tttacaaaat
     7741 tttctcggac acgatcaaag acaacaatgg tatcattcaa ggaataacca ataacggcca
     7801 acaacccagc aagagccttc agatcaaatt caattccgaa caaagcaaaa acgcccagaa
     7861 tcaataccgg atcatgaatc aaagccactg ctgaacttac agcgagttta tattcaaacc
     7921 gcatagcgat atagatcatc gtggctaata aggacacaat aatcgcaaga gcccctttcg
     7981 ttgctaattc ttgtccaact tgcggaccaa caaaatcaac tcgttgtttt actgctccag
     8041 gcagaacttc catcacctga tctaccagca tgctctgctg tttatccgct ctcggtgcaa
     8101 tgctgataag tacatctttt gatgtaccat aactaaccac ttgagcttct ttaaaacccg
     8161 ctttattcag attttctcta attaaaggta aatcagccgc tttggagtaa ctgatttcaa
     8221 tttgcgttcc accagtaaaa tccaatcccc atttcaaacc attaatcatc aatgcgccaa
     8281 tagacactac aaaaattaat atagaaaaca atgcagtcca acggcgcgcg cccataaaat
     8341 ctatttttga atttggatta aaaaattcca tctgagtctc gccttatatg cctatagata
     8401 attttttgac attacgccca ccataatacc aattcacaat agctctggtg taagtaatac
     8461 cagttaacat agaagttaac aaacccagag aaaggacaac cgcaaacccg cgcaccggtc
     8521 ctgtgcccac cgcaaataaa atgatagcta caattaaagt agtcacgttg gcatccaata
     8581 tagttgaaaa tgctcgctca tatcctgcat gaatcgcagc ttgaggagac aatccattgc
     8641 gcaactcctc tctaatccgc tcataaatca ggacattggc atcaacagcc atgcccacgg
     8701 tcaaaacaat ccccgcaatt cccggtaaag tcagtgtcgc atcaattatg gacaacaatg
     8761 cgcaaagaaa aactaaattc agcatcaatg caatatcagc aattaaacca aaaaaatgat
     8821 aatacacgag cattagtacc aaaattaaac ccataccaac ttccagggaa ataagtcctc
     8881 ggcgaatatt ttcctttcca agagttgggc caacggtacg ctcttcaaca ggatagattg
     8941 cagcaggtaa agcgccagct cttaataata aagccagatt acttgcttct ttgctatcag
     9001 tcagtccggt aatctggaaa ttgctgccta aagcattttg aatcactggc gcactaataa
     9061 ctcgctcttc tttctttgtt actcgttttt cctcgccatt aattgtttgt gtagtcgttt
     9121 tggtttccac aaaaacgatc gccatgcgct taccgatatt ttcacgcgta actttagtaa
     9181 acaagctctc gcccccgcca cccaattgaa tttgaacaga tggagaagcc gtttgttgat
     9241 caaaactgga cactgctgac gtaatagagt caccacttaa aacgacctgt ctctttaata
     9301 agataggatg accatccatc atatacagct tatcattgac aggaactacg ccggtttgct
     9361 ttgcaatgaa agggtcattt tcctgatcaa ccaaatgaaa ttctaaagta gcagtaccac
     9421 caagtatttg ttttgctctg gccgcgtctt gaatgcctgg caaatctacg gcgactcgag
     9481 tcgccccttg ttgttgtacg acagcttcac caactccgag ctcgttgaca cgatttctca
     9541 agatactcat tgtctgctca atagtatttt gtcttatgga atttaattct gctggagaaa
     9601 tggcgcctaa aattgaattt tgtgttttag ttttaacaaa aaccacatca gatatttttt
     9661 gtcttaactc aactaaagca ttttccattg agtcagaatc tctgaaacgc aattcaatcc
     9721 ctttatctga taaataacgt atgcctgaat aacgaatacc tgcttcacgt aattcttggc
     9781 caatattttt cattattcct tcataacgcc gacttataac actctcgaca tccacttcaa
     9841 gaagaaaatg aacaccgcct cttaagtcca acccttgctt catagggtca gcatgaattg
     9901 cttcaagcca agctggtgtg gaaggcgcta aattcaaagc cactgtgtaa tcagatccca
     9961 ggctattctt gataacatca cgagctaata attgagaatc agtagaagaa aatactacct
    10021 ctaccccatc cctctccaca accagggatt tataatccat attggcattt tttaatatgg
    10081 actctacctg attttttatt gtctctgtat cttcatctgt acccgcagaa atttgaacaa
    10141 cgggattttc cgtatacaaa ttcggtatag cgtaaataaa tccaatgact gcaatcacta
    10201 tcagcattag atttttccat agaggatatt tattttgcat ttctgattcc ctatgcctaa
    10261 cttaaagtga tttgatagtg ccttttggta atactgcagt aactgcgcct ttttgcaaat
    10321 taatttctat accttctgct gggcttactt tgatgtattg atcatccagg ctcacaactt
    10381 ttgccaaaat ccctccggat gttactattt catccccctt ttttaagcga tttatcaatt
    10441 ctctatgctc tttggctctt ttgctttgcg gccttattat cataaaataa aacaagacaa
    10501 aaatcgcaac gatcataatc agtgaaaaag ttccatcagt ttgagcagct ggcgcatttc
    10561 ctgctgcagc taatgcatca ctaataaaaa aactcataaa atctcctcaa atcgggtatt
    10621 aatttcctgg ttttctttcg aagatggtcg ataaaccaat ttcaaaagac agttctaaga
    10681 actcttgtat ggattaccag cgtgacatca tatagcgaat ttactcgaag gtaaatgaat
    10741 agttaattac cttgaacgta atacgcatta tgttgaatca agaatctctg acatagcaat
    10801 ggggtattag ctaacaaatg atgcagataa gctcttgtca actgtgctga acaagtagga
    10861 catgagcaat tctcatcaat cagctgaaaa ttgaaagcct ggctttcatc cgttaaatca
    10921 acattccctt cgcggccgta tacaatgccc cgcatggcat tttttgcagg ctcatccgat
    10981 tcgattaaaa tcgatttctt cctgtttaaa tcctcgatta aatcagcacc gataggacca
    11041 gagacataaa gaggtaattc agaccatttt tgcaattgac tcaagaaatc atcatgatat
    11101 tttgcttcac tgaaattgaa atacacacca tgctttccag gcacctgcaa ggtttcaagt
    11161 tgcttcgctg acaaaaaagg gaatatagta tcatcccatt gctcccatag cccggaaaaa
    11221 ccattcacaa gcgtttcagg taaaagcact gcgacaggct taatatgcaa aatcaaatgc
    11281 actaactctg agttagttaa tttgattcga cttccgtcat aaggtgaaat taataccacc
    11341 atcccgtttt tatctgatac aaggcggctc gcattgagga tgagatgacc tgaccaccct
    11401 agatatttgg tgaaatcagt aatatttttc aataatgaat atcctggctt taataacaat
    11461 aaatccagat aacaagatgc gattgtaatc ttaatttctt gccaattggc cgcagtaaga
    11521 cataatcctg cttcagtatt taaaacagga acaaaatttt gatttgtact caacatccac
    11581 tatcctcaaa ctttagccac cattattata ttccagaaca cgttataaca ataaactggc
    11641 atctccataa ctaaaaaaac gatacctttt atcgattgct tcttgataaa gagccataca
    11701 ctcctggtgt ccaataaaag ccgacaccaa catgaccaga gttgattcag gtaaatgaaa
    11761 attagttatt aatccatcac aaaccttaaa ctgatagcca ggataaatga aaatatcggt
    11821 atcacgacta catggaatta gctctcctcc catagcagcg ctttcaaggc tgcgtaatgc
    11881 agtagtccca acagcaatta ctctatttcc catagatttg gcagccttca ctgcagagca
    11941 cagatcagga ctaaccgtaa accactcact atgcatttta tgatcctgaa tgcgttcaca
    12001 gcgaacaggc ctgaatgttc ctgcccctac atgaagagtg acataagcaa tattaactcc
    12061 tcgcgctcta atactggata aaacagcatc atcaaaatgc aatcctgctg taggcgcagc
    12121 aacagacccc gcgcacttgg catagacggt ttgatatctc tccttatcca gtgattcatc
    12181 ttctctggca atataaggtg gtaaaggaat atgcccgaga tcatttaaaa gatctaaaat
    12241 attttcgctc gccttacaaa gaaataaatc atcctgcctc tctaatactt caagccgctt
    12301 accggcatca agatatatga gatcgtttga ttttaatgat ttgctcgctt tgatatgggc
    12361 caggaaagta aaatcgccag ttatcctctc tactaacagt tcaaccttgc cgccagtggc
    12421 tttgtggccg tataagcgtg ctggtatcac tttactgtca ttcattacca ataagtcccc
    12481 tggctgtaag aaatctgcaa tttcccggaa ttgataatga ccatattctt ctgtctgtcg
    12541 attataaatt aaaagccgtg aatcacttct attcgccaga ggatattggg ctattaattc
    12601 ggaaggcaaa tcaaaataaa aatcttgctt attcatagaa caacctaaaa acaaattggt
    12661 gcaattatac accgaacttt agtcttaaaa tcaattatca ggaatttatt cattttatta
    12721 gaaaattaac atcatttgtt ttttatctgt ttaagagact acgagcaaga tcagcgtaca
    12781 gaaaattaaa atcagggtac ttgttaaata gggataagca taacccttgt aagttcgttt
    12841 gcgatagatc aggtccagta ataatacagg tcccactgaa acagtagtga gtagtataat
    12901 gagtgctatc gatgtggaat atggcttaaa aggcataatc cgcgtcaaaa aagctaacac
    12961 agcttgcaat acttctcgaa tataataaat tacccagata aataaataat caaaattata
    13021 aattagccaa gaagcgatgc ctaaaggaac aagtaactta gcccctttga ttgccaaaat
    13081 ttttttaaag gttctaatga attcttgaga gaagaaaacc ataattgttg caaataaaat
    13141 aaccaaagcc aaggttaaaa gcattttaaa tcctattttt aattttattt aaagatatct
    13201 ttttattgtt tttcacttaa acttatcaat taaccaaata cttttttatt ttttctcttc
    13261 cagctcttcc caacgagtaa aatattgatt taacaatgcc tcttccttgg ctagcagctg
    13321 agttacctct gaaataatct tggcatcttg ctgataaaat tcaggtgagg ccatttgtga
    13381 atgcagtgct tcaatttttt tctctacagc ttcaatctgt tgcggcaact tcgataactc
    13441 acgttgttca ttaaaagata aatttttagc tttctctgga ctgctccgtt ttgaaactgg
    13501 agatttcgtt tgctgctcac ttcgtgttaa ttgatgtttt ttataatcgt cataaccacc
    13561 aacaaattca ttaaacttac catttccttc ataaaccaac acactggtta ctacttgatt
    13621 aataaacgct ctatcatgac tgattagtat taaagtacca ggataatcag ctaatacagc
    13681 ttcaagaagt tccagagttt ctatatccaa atcattagtt ggttcatcca ttacgagtaa
    13741 attaaccggt ttggcaaaaa gttttgccag taataatctg tttctttccc ctccagacaa
    13801 ggaagatact ggctgattaa atcgatcaga ggaaaataaa aactctttca aataactggc
    13861 aacatgtttt tgcttaccat taattgttac ataatcagcc ccttccccca cattaaacat
    13921 gactgtttga tcttcaagca aatgtcggcg taactgatca aaataggcta cctgaagccc
    13981 tgtccctaat tttatagttc cagcatcagg tttaatttct cccagcagta atcgaactaa
    14041 agtagttttg ccacaaccat tggggccgat aatcccaagc ttgtctcctc tagtcattaa
    14101 taaagaaaaa tcttttataa tgggcttgtc gttaattgca taactcactt tgtttgcttc
    14161 tactacaaga gcaccagaac ggcttacatc caaatttaag gatttcactg ttcctaactg
    14221 attacgacgt gctttgtact gctctctcat tgcctttaag gccctgactc tgccctcatt
    14281 acgtgtgcgt cttgccttga tacctgtacg tatccaggct tcttcttctg caagccgttt
    14341 gtcaaacaaa tcattttgtt tttgttcgct aagccttatc gattcacgcc tgtcaaggta
    14401 agtttcataa ttacaatggt gcagatacaa ttttccacga tcgatttcaa ggatgcttgt
    14461 tgctatctgg cttaagaatt ccctgtcgtg agttactacc attacagcac cagaaaagga
    14521 ttttaaataa ttctctagcc attcaattgc ttcgacatcc aggtggttgg taggttcatc
    14581 cagtaacaat aaatcaggtt tggcaagcag tgccgcacct aataatacgc gtcgtttcat
    14641 accgcctgag agattagaca tctgctcatg aatatctatt cccaatcggc ttgccatcgt
    14701 ttcaatttca ggtaataaat gccatgcatt caagttgtcc attcgctgct gacacttggc
    14761 caatttgtcc atatctcctt gttttgtaaa ctcatgaaac tgggacaata cttcccctgt
    14821 ttcacctaag cttttaacaa gaaaatggta cactgactct ccctcagcac ctgggacttc
    14881 ctggactaaa ccagaaatac gcaaaccgga tgagcgttga atttgtccac tatcaagcac
    14941 caactcccct tgtagtattt tcagcaatgt tgattttcca gcgccattgc gtccaacaag
    15001 agcaattcta tcctgtgact gaatctgtaa atctgcacgg tccagaatac aattgccagc
    15061 caagattaaa gaggcaccgt taagggaaac tatactcatc aaaactctct atttaaataa
    15121 atctgtttgt tctcatttta ttgaaaacat acagtcagtt cactgtcttt ttaatatcaa
    15181 ttgtttgccc tgctgaatca tatgaaattg tgataggaca ttcattccta ataacaccgt
    15241 attatcatca ttttccggga ttatgaccgc tttgacattc tgtaagtgaa actctccaaa
    15301 tgacaaatgt tgcaaacgag ttaaaaatcc cgttacctta ccattggcag tttctatggt
    15361 aacaggatat cgcccggtaa tttttaaatc cttagccagg ccttgcggta ttgccaccaa
    15421 agtagcgcca gtatccacta aaaactctac aggatattca ttaatcttac cttttatcca
    15481 ataatgtcct tcattatcag cgcttaatac gtattcggta cgactagcta caaaaatggt
    15541 actttctggc ttatcgtaat agtaaaagaa taaaaacatc cctataaaaa atataatcca
    15601 aacaaataag aacatcagac gccctgtgcg agcgtattga tcatcactca tgattatgcg
    15661 gatgcaggta aaaccagaat cccatccatt tcaaccttgg caccctttgg taatgcggag
    15721 acttcgattg ccgctcttgc aggataaggc tctgaaaaat atcgactcat cgcttcgtta
    15781 attaagggaa aatgacttaa atcagtgaga tagacgttta atttggcaat attggccaga
    15841 ctaccaccag cctcttcaca tacagccgat aaattttcca ggacttgagt aatttgtaat
    15901 ttaatatcat cgctgcagat ttgcattgtt tcaggatcta atgggatctg tcctgaaaga
    15961 aaaacgatct caccacttct tatcgcttga ctgtacgtgc ctatcgcttt tggcgccaat
    16021 tttgtattga ttggttgcat taaatctccc cttgtttttt tcgatataac cgcataacta
    16081 ctttatttgc ccttaatcga cgcatgactc gagccaaatg ggttctgtcg cgaacactga
    16141 tggaaaaagt gaccgcatta tgacgaccat ctctgggatc cacattaata ttccctatat
    16201 tagagtctgc ttctgaaata gcagttgcta aagcagccaa tacacctctt tcgttggcta
    16261 catctacagt aatatctacc caatattcac cctgaacctg ctcatcccaa cgcaaagcaa
    16321 taaactgttc cggattacta cgagttgttt ttagtgtagt gcagtcactg gcatgaacaa
    16381 taatgcctct gccttgttga aatttaccaa ctatattatc tccaggtatt ggttggcagc
    16441 attcaccaaa atgcacaacc atcccttcag tacctttaat ggccaaaggt tttttattgc
    16501 ccttatccga ttcattggcg tcttgcgaaa ccaccaaccg cttggcaata accattgcca
    16561 tctgattccc tattcctatg gcatacaata agtcatcaac cgttttatag tgtaaatcgc
    16621 tgagcaatgc ctgcaatgtt tccggaggca ctttggcgta atcgctggat aattcagcaa
    16681 gggattgttc caataatcgt ttgcctaaaa caatagactc agcgtgttgt tggcttttca
    16741 gaaaatgacg aatatttgat cttgcctttc cagtcacaac aaaattcagc caagccgggt
    16801 tggggtgtgc tcccggggct gtaataatct ctacggtttg accattggat aaaggaatgc
    16861 ttaaaggaac caaacgcctg ttcactttcg ctgcaacaca actatttccc actccagagt
    16921 gtaccgtgta agcaaaatct acaggagtgg ctcctttcgg caactccata atgtgccctt
    16981 taggagtaaa aacatatact tcatcaggaa ataaatctat tttaacgttt tcgataaatt
    17041 caagagaact gcctgtgctc ctttgcattt ctagcaaact ttgaacccac tctctggcac
    17101 gaagttgagc ttcattgact tccaatccgg aagatttata gatccagtgc gctgctacac
    17161 cattatcagc tactttatcc atctctcttg ttcgtatttg aacctcaaga ggaacaccaa
    17221 aaggcccaaa caaagtggta tgcaatgact gatagccatt ggctttggga attccgatat
    17281 aatctttaaa tcgttgtggg acaggtttat aagtacagtg caaagctccc aaaattctat
    17341 aacaggagtc aatgtcttct gtaatgatgc gaaatgcgaa cacatccgta atttctgtaa
    17401 aggatgcttt tttctgcttc atttttctat aaatactata taagtgcttt tgcctgccaa
    17461 atacttgttc atagggaatg ttcaattgcg ataaggcgtg ttgcaaatct ttctctatgg
    17521 tctgtgtcaa ctctcttcga tttccccgag atttttcaac agcagactta attgccctgt
    17581 aacgcatagg atataaagct tgaaaaccca aatcctcaag gccgatataa atagaatgca
    17641 tacccaaacg attagcaata ggggcataaa tctcgagcgt ttcaattgca attcgtctgc
    17701 gcttggctgc gggcattgat cctaaagtgc gcatattatg taagcgatca gccaatttaa
    17761 cgataataac gcgaatatcc ttaaccatag ccaaaaccat cttgcgaaaa ttttccgctt
    17821 gggcttcggc ctttgattca aatttaattt tggtgagctt ggtgacgcca tcaacaaggg
    17881 cagtgacttc ttcaccaaat tgattaacca gttcttcttt acttatagag gtatcttcaa
    17941 cgacgtcatg aagcagagta gccatgatag tttgatagtc tagcctcatt cttgccagaa
    18001 tgagggctgc tgctacaggg tgtgtaatat aaggctcacc tgaacgacgc atttggccat
    18061 ggtgtgcttt ttcagccacc aaataagctt gataacattt ctctatttgt gcctgctcaa
    18121 ggtagcattt aagctcttca tcgagctctt taaagtagct cacgcagtac tccctagtat
    18181 ccaacatggc tatttatacc tcacattact caaaatacgg ttttatgcct cgcccttgtt
    18241 ccctttgctt tgcttaaata ccataacttg attatcaaga ggtatatttt agaatggcta
    18301 atattcccaa ggcaaagtca ataactcatt ttaatcttca tccaaaatgt ccgctgtaac
    18361 ataaccttcg gcaatttccc taagtgctac tacagtaggc ttatcatttt cccattcaac
    18421 cataggttga tcgccacgaa ctgcaatttg ccttgctcgt ttggtagcaa ccataaccag
    18481 ctcaaaccgg tttttaacat gttctaaaca atcttcaact gttactcgtg ccataaaatc
    18541 cacctccagg ttattagaac agatactagc tcaaatcttt tattttactg cgatgacaag
    18601 aggaaagaaa gcaatttggc ttgtttattt acttgttttt caattcgaag cctattggca
    18661 attactatcg actgtaattc cattgccgcc ttttcaaaat catcattaac aatcagataa
    18721 tcaaattcag gataatggcc aagttcatcc tgtgctttgg tcattctctc actaatcaca
    18781 tgatctttat cttgtcgcct gttcatcaag cgttcattta atgcgtctaa agaaggagga
    18841 atgataaata cactcactgc atccggataa gcgtgtctaa tttgctccgc tccttgccaa
    18901 tcaatatcga gaacgacgtc aattccaaat tgtaaccgtt tgttaatttg aacaactgag
    18961 gtaccatacc aatggttaaa tactttggca tgctcaataa acgccccctc attcaccata
    19021 gaaataaatt gttcctcatc aatgaaaaaa taatccaccc cttctttttc tccaggcctc
    19081 ataggtctgg tcgtatgaga aaccgatact tcaatctcac ccaccatttc aatgagtttt
    19141 ttcaccaaac ttgtttttcc tcctccggag ggtgctgcaa caatatataa atttcctgaa
    19201 ttatcactag ccatatcatt tactcaaaat tctgaatttg ttcgcgcatc tgctcaatca
    19261 atactttcat ttcaacagca cataaagtta actctgctga atctgatttt gagcttaggg
    19321 tattcgcctc tctatttaat tcttgcatta aaaaatccag tcttctaccc gtcacctttt
    19381 ctgcattcaa agtgcggttt acttccgcta tatgagtctc taatctgtcc agctcctcac
    19441 tcacatcaag ctttgctaat agcaaagcaa tttcttgctc tacacgcgcc tctgtaacgt
    19501 ccatttgcaa ttcatgcaaa cgcgtaagta atttatcttt agtcttggca ctcattgaaa
    19561 ccataatgct tttggatttc attacctctt ggcgcaaagc ctgcaatcgg tcttcaacat
    19621 atgcctttaa tgacctgcct tctccagctc tgacttcaca caatttatcg atacttttct
    19681 gaaataactc caaagcaatt tgagacaact cctccatatc cagcggatta gcctccacaa
    19741 cacccggcca tgaaagcact tgactgacag tcatatcatt agccagatga tgagaggcgg
    19801 ataatttacc gcttaaatca agtaatgcat taatcgtacc tatattaata agcatagacc
    19861 gattatcaca acttgtgtcc ttgtacttta gatggcactc tagtttaccc ctatgtatct
    19921 tatcccgaag caaggatctt aactctgctt caagaaaccg aaaggactcc ggcaaacgaa
    19981 atgagacatc gagatagcga tgattaacag acctgatttc ccaacaaaaa tcacctacat
    20041 caaactgctt ttgtattcga gaaaaagccg tcatactatg aatcataaag aacacctact
    20101 ttttaacctt gcgcgaatat acctgaaatt aaaagaattg cacagggatt gcccatcaat
    20161 taattgcaac aaacttctgt atcaggtcct gaaagtcagg ccttaaacca aacttgaaac
    20221 ttaaaacaaa ctgtttctaa cgtaaagaat ggagacaaat cgagtggttt ggttaaaaat
    20281 atttttcagc aaaaatagag aggaatgtac aaatagcgta tgtatttagg gtgcaactca
    20341 agtattaaag agttatactt gggcatttta cagaggagta tctctaatgc gtcccagtaa
    20401 tcgtgaacat gatcaacttc gtcccgttac aataacacgt aattttacca attatgccga
    20461 aggttctgta ttagtcgaat ttggtcagac aaaagtgatt tgtaacgcat caatcgtcga
    20521 aggagttccc cggtttttaa aaggtaaaaa ccaagggtgg ataacagctg aatatggcat
    20581 gctcccacgt gcaactcata gccgtaccga aagggaagcc agtaaaggta agcaaggtgg
    20641 cagaacattg gaaattcaac gtctcatagg gcgttcattg agagcatgta tcgatttaaa
    20701 ggtattgggc gaaaatacta ttactcttga ttgtgatgtt attcaagcgg atggaggaac
    20761 cagaacagca gctataactg gcagttgtgt ggctatgcga gatgccattc actggatggt
    20821 tcaacgtgaa aaaataaaga agatgcctgt ttttaattat gtagctgccg tatctgtggg
    20881 tatttacagg ggtcaacccg tgctggattt ggattatgca gaagatgttc ttgctgaaac
    20941 agatatgaat gtggttatga atgaacaagg gcattttatt gaagttcaag gcactgctga
    21001 agataactcc tttaaccgag aacaactgaa tagcatgttg tcattagctg aaattggtat
    21061 tcctcaattg attgaaatcc aaaaaaatgc ttgagagcaa tagccctgca taaacagtcg
    21121 tctgctatat aaaattaaca cttcaagcct gaacaaaaga tctctgcagg gacgctttga
    21181 ggcaactgaa gagatcttat tgcagttatt ggcattgctg tcttcaaggt tttttggggg
    21241 aattctggaa cttgcctggg ctcaattcct gtaaatacga acaacgtatt gaaatctatc
    21301 tggacttgcg ccccccaatc tctttgttaa ataatattta aaattcagtt acttagttta
    21361 tataaacttc ttcattcaaa acattttttg cctcgaccat ggggtttttc gcaagccctg
    21421 tctatttaaa ataatgactt gttcagagca gcctcatgag cggtgtgttt cgaaataata
    21481 tttttattaa ccaattcagt caaatgttga tccagtgttt gcattccttt ggcttgccca
    21541 gtttgaattg acgaatacat ttgcgctatc ttatcctcac gaattaaatt acgaatagcc
    21601 ccggtacaca tcataatttc aagcgccgca acccgaccac ctttattctt ttttaataaa
    21661 ctttgagcaa cgacagcttg taatgactca gaaagcatag agcgtaccat cgatttttct
    21721 tcagctggaa atacatcaat tatcctgttt attgtttttg ttgcggaatt ggtatgcaat
    21781 gttccaaaaa ccaaatgccc agtttctgct gcagtcattg ccaggcgtat agtttccaaa
    21841 tcacgcaatt cacccaccaa tataatatcc ggatcttcac gtaacgcgga tctcaaagca
    21901 gcattaaagc tcaacgtgtc tttatgtact tcgcgttgat tgaccataca ttttttgctt
    21961 tggtgaacaa attcaatggg atcttcaact gtcaaaatat gctcataacg ggttgagtta
    22021 atataatcga tgactgcggc aagcgtggta ctttttccgg atccagttgg gccagtaacc
    22081 agaaccagac ccctggaaaa actagccatt tctttaaaaa ttgggggcaa ccctaaatct
    22141 tccatgctaa gaatttcaga aggaatagtt ctgaaaacag cagcggcgcc gcgtgattga
    22201 ttgaaagcat taaccctgaa ccgcgccaga ttggctattt caaaggaaaa atctgtttca
    22261 agaaattctt catattcttt tctttgtctg tcattcatta tgtcataaat aatcttaatg
    22321 acgtctttat gctccaaggg aggtaaatta attttgcgta aatcaccatc aacacgaatc
    22381 ataggaggca tgccagctga aatatgcaaa tctgatgact tgtttttaac tgaaaatgcc
    22441 aacaattccg cgatatccat actctataat ccctgccaat tttttcatta tatattgtag
    22501 attaacccat gaaatgatta ccagcaatca tggatatctt tttgtagtac cattacactt
    22561 catgcataat taatacttct ttttataata atgcgcctca tcttttgatt aaccaggatt
    22621 aatatgagta ttagtcagaa cataaaaaaa gtaagagaac tgttaaccca gacagaatta
    22681 tccagtaaca ggcaaccgaa cagtgtctta ctattggctg ttagcaaaga acaaagcccc
    22741 gaattaatca gagaggcctt cgaacaagga ataagacact ttggagaaaa ctattttcag
    22801 gaagcccagg aaaaaatatt ggcattaaaa gatctgccta tctgttggca ttttataggg
    22861 cctattcaaa gtaataaaac caaaggaata gcgaaatatt ttaattgggt ccacagcatt
    22921 gaccgaaata aaacagcgaa acttcttaac cagttcagat ctgagcaact accaccgctc
    22981 aatgtctgtt tacaaattaa tttggctgga gaaaccacaa aatcaggcat tcctccagaa
    23041 catgccatcg accttgctct cgaagtaaag caattgccta atttgcaact aaggggactg
    23101 atgacaatcc ctcctcagca aaacaacatg caaacacagt acgatttatt ccttaaactc
    23161 aatcaactca aggaatccat caataagaca cttcatttaa aaatggatac tttatcaatg
    23221 ggtatgagtg atgatttggt tccggcaatt aaagcaggtt cgaccatagt tcgaattgga
    23281 cgagcaattt ttggcgaacg aaaaggtaaa ttattatgaa tatcagtttt attggttatg
    23341 ggaatatggc gaaagcaatt gcctgccact tacaaaagca aaacaaatac tcattgtcag
    23401 ctgcctcacc atccttatct gcgggcatta gtaaagaaaa tattcgcact cattacgata
    23461 ataaagaagt ggttaaagac gcggacgtca ttatattggc agtaaaacct acacaaatgc
    23521 gtgaggtagt caatgagatt aaccctttta tttcatcagg ttgccttcta atctcagtgg
    23581 cagcgggatt gtccctgacc tggtttgcca aatattgtaa aaaaggacaa gcagtcgtaa
    23641 gaaccatgcc taataccccg gccatagttg gatttgccgc aacatcaatg attgcaaatg
    23701 aatttgtaaa cgagaaacaa aaaaaatggg cagaacaatt attttcaagc atcgggatta
    23761 ctgattgggt taaagatgaa acagacatgg acaccctgac agccctttct ggcagtggcc
    23821 ctgcttacgt atttttgttt cttgaagcga tgattaatgc aggggtccaa ttgggcctgg
    23881 aagaaacggt tgccaaacac tttgccatac agacattttc aggatcgctt caattagctc
    23941 aacatagcca gttaagtttg actgaactga gaaaaaaagt aacttctccg ggaggtacaa
    24001 cagctgcagc tctggaaatc ctgcatggag atttggatga gctcattttt tctgccatga
    24061 aagctgcgaa acaaaaagcc catgaattag gaaaagttga atagctcttt tgagttaaca
    24121 taaattctat cgcaagaagt cctctgttga cttcttttat tttaatgtca gtcaggattt
    24181 acaaaaaccc caaggtcaag gcaaaaaatg tttttaatga gggagttaag ataaactaaa
    24241 cgactgaatt aaaaat
//
LOCUS       DATNSN010000033        23996 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.033, whole genome shotgun sequence.
ACCESSION   DATNSN010000033 DATNSN010000000
VERSION     DATNSN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23996
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.033"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            1..1016
                     /locus_tag="VAH76_001097"
                     /pseudo
     CDS             1..1016
                     /locus_tag="VAH76_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948076.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2372 family Dot/Icm T4SS effector"
     gene            complement(1781..2623)
                     /locus_tag="VAH76_001098"
     CDS             complement(1781..2623)
                     /locus_tag="VAH76_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385743.1"
                     /translation="MTSLLNCSFITGTRLTEQERLIALIEKHASTSNRRVFHLDFEGD
                     CYVVKMQQKKRSKIGYYILTMIAKVFSIPALRGIPILGGKPTQDIEIKRLASLSAAGI
                     LVPKVVYEGQNYFVMSCLGNSNFDFLLKNPGPQSLVFYWKQALAAILDVHHKGAYLSQ
                     ALVRNMIAQEGLSVGFVDFEDDPGAVMPINLAQTRDWLLCILSSSLRLDISPQKQAEI
                     ILSYLKQDRIDVQEEVFACASKIALLRFIFRRAKLYHNRDLQSINAFIQVMRELITYR
                     SASS"
     gene            complement(3100..4494)
                     /gene="fumC"
                     /locus_tag="VAH76_001099"
     CDS             complement(3100..4494)
                     /gene="fumC"
                     /locus_tag="VAH76_001099"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEO1385744.1"
                     /translation="MAKTRVETDSMGEIEVPSDKYWGAQTERSLHHFNIGKDIMPREV
                     THAFGILKKAAALTNLELGKLPKDKADLIVQAAEEVSKGLLDEHFPLHVWQTGSGTQS
                     NMNANEVISNRAIELAGGTLGSKSPIHPNDHVNMSQSSNDTFPTAMHIAAAIAFNEKL
                     LPAVRNLRQVFAAKMDAFKNIIKIGRTHLQDAVPLTLGQEFSGYVAQLDACIHRLEEV
                     LPELYELAAGGTAVGTGLNTHPQFAVKVAEHIAKITKLPFVTAPNKFAALASHEPLVH
                     AHGAMKTLACALMKIANDIRWLASGPRCGIGELIIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDTAVGIADSQGNFELNVFKPVIIFNVLHSLNLLADSCHSFQEFCAEGIE
                     PNQPVIDYYLHHSLMLVTALNQHIGYDKAAKIAKTAHHDNISLQEAAVKLGILTAEQF
                     AAFVKPEKMISPEQ"
     gene            4739..5473
                     /locus_tag="VAH76_001100"
     CDS             4739..5473
                     /locus_tag="VAH76_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2936 family Dot/Icm T4SS effector
                     methyltransferase"
                     /protein_id="HEO1385745.1"
                     /translation="MVVRTIRIYQPGEYQPGQVLELSPEAGQHVGVVLRMEQGEQLTL
                     FNGDNQEFTAFIERVKKKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEVVMQKSA
                     ELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQ
                     FVREAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEITLTSGYGFLPLS
                     LGPRVLRTETAAITALSVLQAAGGDL"
     gene            5686..6012
                     /gene="trxA"
                     /locus_tag="VAH76_001101"
     CDS             5686..6012
                     /gene="trxA"
                     /locus_tag="VAH76_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO1385746.1"
                     /translation="MSDHIKTVTDASFEQDVINSSKPVLVDFWAEWCGPCRALTPILE
                     EVAATHSKDMTFAKINIDENPQAPAKFGVMSIPTLILFKNGQVEAVKMGLLSKSQLSA
                     FVESNL"
     gene            6335..7597
                     /gene="rho"
                     /locus_tag="VAH76_001102"
     CDS             6335..7597
                     /gene="rho"
                     /locus_tag="VAH76_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEO1385747.1"
                     /translation="MNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHAL
                     KGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIR
                     PPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTAR
                     VVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDE
                     CDAIEFLLERMKNHKTNAEFFDAMKRQE"
     gene            7743..9209
                     /gene="ubiD"
                     /locus_tag="VAH76_001103"
     CDS             7743..9209
                     /gene="ubiD"
                     /locus_tag="VAH76_001103"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEO1385748.1"
                     /translation="MKYSDLRDFIAQLESRELLKRIDYPVSPHLEMTVVSDKVLRSGG
                     PALLFTNTPNYNMPVLTNLFGTVERVALGMGEESIVALREIGKLLAALKEPDPPKGFK
                     DAFSKLPLLKQALNMAPKYVSGAECQTHVWEKDEVDLTLLPIQTCWPGDVAPLITWGL
                     VTTRGPHQSRENMGIYRQQLLSKNKLIMRWLSHRGGALDYQAWQQEYPQERFPVAVTL
                     GADPATILAAVTPVPDTLSEYAFAGLLRGQRTRLTRCIGNDLHVPASAEIVLEGYLEP
                     GNEAPEGPYGDHTGYYNEVQSFPVFTVERITHRDKPIYHSTYTGRPPDEPAILGVALN
                     EVFIPLLQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYPGHAKRIMMAVWSFLRQFMYT
                     KFVIVCDDDVDARNWQDVIWAMTTRMDPSRDTVMVENTPIDYLDFASPVSGLGSKMGM
                     DATSKWPGETQREWGKPITMDEDVLNRVNGYWSLLGLK"
     gene            9209..9922
                     /locus_tag="VAH76_001104"
     CDS             9209..9922
                     /locus_tag="VAH76_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HEO1385749.1"
                     /translation="MTDKIIQAQVEDISPLTDSIVRLVLMPDEYVDYQAGQYLQILFA
                     NDAFSYSIANAPLGSHRYELHIRHSQDNDYHQSLLAHIKQYGSLRLRLPLGECSIDQL
                     HPQKPILFIAGGTGFAPIKAMIEQLLADSDSRPFELFWGARSQSDLYMDEKVRSWGSH
                     ASHFQYFSLLSDKSEDTLASRVLARHANILRDWQIVISGPFDMVYSTRDTLVGYGISP
                     TQMFSDAFSFESKTDDAKN"
     gene            9940..10134
                     /locus_tag="VAH76_001105"
     CDS             9940..10134
                     /locus_tag="VAH76_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385750.1"
                     /translation="METLYQILGLIGAGLIIFILYRFIKGSPEQFSKENMSKSFMTMG
                     VLGLILIGFIALLVLMLRNT"
     gene            complement(10172..10483)
                     /gene="hspQ"
                     /locus_tag="VAH76_001106"
     CDS             complement(10172..10483)
                     /gene="hspQ"
                     /locus_tag="VAH76_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEO1385751.1"
                     /translation="MNKIAKYNIGDLVIHKRIRYRAIVIDVDPIFQASGKYNPRATQH
                     EFATRNPWYRLLVDDSSQITYVEECQLLPDTSQEPINNPNIDDYLIKNKGSYQKAIPS
                     H"
     gene            complement(10545..11270)
                     /gene="tatC"
                     /locus_tag="VAH76_001107"
     CDS             complement(10545..11270)
                     /gene="tatC"
                     /locus_tag="VAH76_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEO1385752.1"
                     /translation="MSFMLIHLIELRQRAIYTLLWFGALALLFFFISDQLFHLLVRPL
                     LRSLPNQEGLIATQITSSVFTPLKLAVDTALLLTAPFALLQLWRFMRPGLYKNEQEQI
                     RGTIILSLLLFVFGTLFCFYLVLPYMFQFFAQALPEGVRLMPDMAYAIDFITRMLLLF
                     GFSFQVPLICLVLVKTHFTTVETLIKIRPYVIVGAFIIGMLLTPPDVFSQVALALPLC
                     LLYEAGIILARIYGKSPAPTLIQ"
     gene            complement(11376..11777)
                     /locus_tag="VAH76_001108"
     CDS             complement(11376..11777)
                     /locus_tag="VAH76_001108"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEO1385753.1"
                     /translation="MAYRKMLKSKIHRACVTQADLDYEGSITISPELLKVANILPYEA
                     VNVWNITAGTRFETYAITGEKGSTDICVNGAAAHLVTPGDLVIIASFTQILEEDCAAH
                     EPTVVFVDQFNRLKEIRPERIGVKSRIPYPA"
     gene            12113..12883
                     /gene="rsmA"
                     /locus_tag="VAH76_001109"
     CDS             12113..12883
                     /gene="rsmA"
                     /locus_tag="VAH76_001109"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO1385754.1"
                     /translation="MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGA
                     LTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLNLIPADALTVDFCQFGPHLRVVG
                     NLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
                     EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNN
                     LKGIISLSQLNDLGIDGGKRPEQISVAEYVQLAKFISN"
     gene            12920..14257
                     /locus_tag="VAH76_001110"
     CDS             12920..14257
                     /locus_tag="VAH76_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HEO1385755.1"
                     /translation="MFHRQRKSSLSAQSKSLKDLIRMVTAEQFLSEEKRQVLLQKMRA
                     FSGLEASRYESLCSVLVENLVNYCQNLPETANSYYSQPGGLVDHALNRTEAALSLFKE
                     FTVQEQPDLLSEEQKLWQYALYSAAILQGLGKLFDEFRINLFDSNGQLLKQWNPLLES
                     LIQTGSYYDYQFEKEADAEFRRRLNLLLARTLMPASGFAWIASNPEVLAVWLALLNED
                     YRSAGTLGAILIRADAIAIQRYFLAFMARSGARRSGRYGRAVTFTGGVPESITEKEQA
                     IGVEFIQWLIKSLDEGRIMVNKAPLFMVPGGMLMCPEMFQLFVREHPEYKNWQAIQNG
                     FLSLGLHGRDAGGNVTSRFEQAHTQQMYTGVVFAGYGVALPNSVQVHHLNTGKIESMS
                     ALELIHKSQYTSQFTQQSSAIAIAPLQRLAANGQWQNIEQDMSQLTFGAKRGA"
     gene            14250..15080
                     /locus_tag="VAH76_001111"
     CDS             14250..15080
                     /locus_tag="VAH76_001111"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEO1385756.1"
                     /translation="MPDYAIGDVQGCYDPLQRLLELIDFNEKEDCLWFVGDLVNRGPD
                     SLAVLRFIYSLPVKPKITLGNHDLHLLGLLFGGQPWKGHDDTLEEVMLADDGEELGHW
                     LRKQSLLCRSSELNIVMCHAGIAPLWDLSKAVGLANELEAVLSGDSYHEFFAQMYGNK
                     PDIWSDDLVGLDRLRVITNYFTRMRYCDAHGRLDLGYKGTLSKAPNHLYPWFEVPCRK
                     EIEMDIVFGHWAALMGRSSHPRIHAIDTGCLWGGQLTALRLQDRQRFSVPGYGVSRFE
                     "
     gene            15124..16752
                     /locus_tag="VAH76_001112"
     CDS             15124..16752
                     /locus_tag="VAH76_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1385757.1"
                     /translation="MKFLNKSTALLPVIILSGLISFHAASAKTSSNADNSLYSWTENP
                     SAEAQRVEQAEERLKAAIANPEYMLKNWIELPTSPESKKKTRHQLTIREAILLALRYN
                     PNIQNAELDRIIQRYQLRLANNEFELQYALGASGVVQKSTFEGIGSSTNHSFLASPEL
                     DLKTKIGTKASVSIDNNVSTNNSYNPVLNLSITQPLLRGFGKSANEAKLLDAIDKEWL
                     NKLSLQQSVMDQITQVITAYRALILSGNNLENQRLQLEEARKSYRINEKKIAAGQLEP
                     TGNIQQSYQIESLSLMVEQGENDFKLASQELLQMIGLDPDLHISVPSDVTIKEIIVPD
                     LQESIAIALSHNTQYLAQKMALRADERAYTVAKNQQLWQLDLAGTVQGGAVNDVTGTS
                     SGLSSIYNGNNITESARVTLTIPLNDIGRRSQLINAKVQLEKDKINLIAAKRALITSV
                     TNTINNIQSLAKRYELAQKQVKLAEQSYALEKKKQQAGISSALDVNNTQNQLIQAQSG
                     LIGAKIAYLNQLSALQRILGTTLDYWQIKLRYGG"
     gene            16749..17894
                     /locus_tag="VAH76_001113"
     CDS             16749..17894
                     /locus_tag="VAH76_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385758.1"
                     /translation="MNNYLKIFSMVSLILMLVSCGSSDKPQTSSLKTYEVKPQPLHKA
                     LHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNELQKQYNDTLTD
                     YLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQATRKLSEMLE
                     KMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDKNNRVTV
                     GSTIKSGQVIALIGDLNGISVEIDVPEIDIDKIRPGMEATISGVAFGRHQLKGKLVAV
                     NAQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLLIPIAAIK
                     REKGNSVVQVQLAKGKVQKRMITTGAAQADSVVVESGLKSGDVVVYD"
     gene            17887..18576
                     /locus_tag="VAH76_001114"
     CDS             17887..18576
                     /locus_tag="VAH76_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385759.1"
                     /translation="MIDRPLIQLSDLVKTYHLEGISTTVLKAVSLTVNEGDLLAIVGA
                     SGSGKSTLMNIIGLLDKPDAGTYTLNNRNVASLSDDEAADLRNQNIGFVFQQFNLLPR
                     FTAMQNVALPLTYRGVNPTLIKEKVEQALDKVGMRQYIRHRPTQLSGGQQQRVAIARA
                     LVTDPQVILADEPTGALDSRTGSEVMNLFLALHQEGRTIIMVTHDEHVAAQCKRRITL
                     ADGAIIAESGQ"
     gene            18573..19766
                     /locus_tag="VAH76_001115"
     CDS             18573..19766
                     /locus_tag="VAH76_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385760.1"
                     /translation="MNFLNHCQQALVNLTASKLRSLLAVLGILVGTAAVVALISCGQL
                     ATEKALEQFKALGTDLLAVSVYQKMPSKSHSESDSLTIDQWQQIAERIPYVLRIAPYS
                     TAYQTLSFNGKVMQGAVIGADESLADIIHVTLAQGHFVSFLDSFEHFCVIGDSLAQQI
                     KEITLDDPIGKQLRIGQSLYTIIGVAERWKENGFFNEDINQAVIIPLAGMSLVSKDAK
                     INNAVLLLKPDSPIDEVINEIKEIINSQAPKLSVFPRSAKQIIASMENQGRIFTLLLA
                     VIGGISLLVGGIGVMNVMLVSVSERKKEIGIRKAVGAKNREIQALFLVESVMLSLLGG
                     VLGVILGLIFTRIVAYFSDWTFTIYLLPPIAGFLVSAATGIFFGFYPARRASKLEPMV
                     SLRSE"
     gene            20101..21063
                     /locus_tag="VAH76_001116"
     CDS             20101..21063
                     /locus_tag="VAH76_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEO1385761.1"
                     /translation="MKKWGLAIVALMLFNQITYASQSWNQWVAGVREEALQQGIPPSL
                     FDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKRNKEILEE
                     IAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
                     LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANY
                     MKQNGWQAGEPWAIQVKLPAKFDMKLEGKGIVKPVSEWNALGVRTESGEPLPYQNLQA
                     SIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ"
     gene            21372..22418
                     /locus_tag="VAH76_001117"
     CDS             21372..22418
                     /locus_tag="VAH76_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1385762.1"
                     /translation="MNQKSFQWSVVGAGPAGIAAVGKLLDYGIAPSEILWIDPHFKVG
                     DFGQLWRNVSSNTTVKLFSSFLHAANSFSYKKAPSTFRLNNLDPDNTCLLSDVAEPLQ
                     WVTSVLVNQVIAEETVIHSLSLSGGCWTLCSDTQQYRAKNVILATGAVPSSLNYPGVN
                     VVPFEIAIDKQRLAHFVDTNETYGVFGSSHSAIMILRSLIELGVKKVINFYRSPCRYA
                     INMGNWILFDNTGLKGDTAAWARKHIDGVLPANLVRYYPSELNIARHLPECDKVIYAV
                     GFERRKNMTIDNYEHVTPNPHVGIIGPGLFGLGIAYPELSVDPFGTIEHQVGLWKFMV
                     YLCKVLPVWFKYPA"
     gene            22528..23031
                     /locus_tag="VAH76_001118"
     CDS             22528..23031
                     /locus_tag="VAH76_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385763.1"
                     /translation="MTKILICLGLFLSSNLLMAKPLSQLDSANLLPCFNIEQAERIGK
                     QINKLLQHEFCEENSNPKKFASISHNILPKIMTETFLGVTPPENWQQLSDDIIKNCIA
                     NKNLCKKAARKELEECIKPRIPLILIQFGPWLAQNCPQLNKSLIEQWPSKQTILKKII
                     NENKSVE"
     gene            23274..23821
                     /locus_tag="VAH76_001119"
                     /pseudo
     CDS             23274..23821
                     /locus_tag="VAH76_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         7113 a         4633 c         5330 g         6920 t
ORIGIN      
        1 ttaaaacatc ataatcttgc aagagtaatt gtgggagatt taggtcaggt catcgaaaaa
       61 aatgatctca atatgaaact gataattaca aataaggata cgacacaaaa tatagttgaa
      121 cttgaaatga ctagtaatct caagaaaaaa gagttttata aaaaggggat cgtgatagcc
      181 acagatacat cagctatcaa tcatgaatta atgaaatatg ttagtgaagt cacggttaat
      241 tgtcactggt ccaggaaaaa tagaacatcc tacaacagca cgagggagct agttgatact
      301 gttgccaacg aactgaagca tgagaagcag tatccgcggc tcaagcgggt taccatttta
      361 ggctgcgaca cagcggatat aatccctccc gaggttgaaa ccaaatcatc ttctacaatg
      421 ttatatgtat accaatgtga aaaaaataac gcagctaccc cagacaggaa aaaaatacaa
      481 agtttccctt gttttgtact cacaatttca caaggacaat atcagctcca ttattatatt
      541 ggccaagaca aagaagaaac ctgtggagaa atggttaaga ttactaagca aaaaaactcc
      601 aaaataacta taaggtgcta gcagtttgca ataaaaatga catccccctc atacccgata
      661 gtatcttaaa gaacatttat gataaaacaa attccgcaaa aataaatggt gtaaataacc
      721 cctacttaca tagaggcgct accgtaggca atgtacaatc aagagaagag tggagaaatc
      781 ggaaaaaaaa tattcccgca ctctcgtgtg gtttatttaa atttgataat aaaaaaattc
      841 acaactcaaa tttagctgaa agggcagctt taattatcaa taatagccgg aataacgtta
      901 aagttaaagg ctatttaata ccaattaccc cggatgaaaa ccacggattt atgaaaccaa
      961 tgacaatcca tgaggacacg ccttgtaaag cagttataat tgaccatggg cagtaaaagc
     1021 agtgtagatc aattgcatga cagcgctgat agcagctaaa gaacattttt cagtgtcatt
     1081 tcagatcctg aactaaagca gtcagataaa ttagctaagc cccgaagaac acaccagcct
     1141 taattgaggc gtattcttat ataaaaataa aaaattgtta acatttctca aacttacgaa
     1201 aaataggctc taatttaagt tgtttttttg gctattagta ccctgtctgg tatttttgat
     1261 ttccttcatg cataaacgat gtaactcctt aataaaatca acaatcaatt ttggctttaa
     1321 agcaaaattt tcaagtatat aacataatgc tttggtcatg cccatcaagt aaccagtctc
     1381 tttgctttcg aaacctaact agctttggtg catgtccaaa tgagacttta gcttttcttt
     1441 atcggcactt ttacccttga gattgagaaa ctctaacacg gagcgactta atcctcgttc
     1501 ctctagaaga tcttggtatt cttgtatcgt aatctcttga aatagcgcat cttttaaaat
     1561 agtttccgta ggaattattt ttaatttgtc atcatctaac aatgttagct gtttatatac
     1621 aaatcgatac agttattcgc gataatcata acgaccacct tcaagcgcat aagtagaata
     1681 aacctgatct tttaatacag ttcaagttat ctctctaaat ctatagatta atttttgtat
     1741 ttatctgatt tatcttgttt ttgcggcata caagagtttt ttaactgctt gcagatcgat
     1801 aggtaataag ctctcgcatc acctgaatga atgcattaat tgattgtaga tcgcggttat
     1861 gatacagctt cgctcttcga aaaataaaac gtaataaggc aattttagaa gcgcaggcga
     1921 atacttcttc ttgcacgtct attctatctt gtttcaagta acttaaaatt atctcagctt
     1981 gtttttgggg actaatgtcg agccgcagag aggaggataa gatacataat aaccaatcgc
     2041 gtgtctgggc taaatttata ggcatcacag cacctgggtc gtcttcaaaa tcaacaaagc
     2101 caacagatag tccttcttgg gcaatcatat tacggacaag agcctgactt aagtatgctc
     2161 ctttgtgatg aacatcaaga atcgctgcta gcgcttgttt ccaataaaaa actaaagatt
     2221 gtggaccagg attttttaac aaaaaatcaa agttagagtt acctagacaa gacataacaa
     2281 aatagttttg tccctcataa actactttcg gaactagtat ccccgcagcg gataatgaag
     2341 ccaggcgttt tatttcaatg tcttgagtgg gtttgccgcc aagaatagga atgccccgta
     2401 aagcaggtat ggaaaatact ttcgcaatca ttgttaaaat gtaatatcca atcttgctgc
     2461 gtttcttttg ttgcatttta acaacgtagc aatccccttc aaaatccaag tgaaatactc
     2521 tgcgatttga agttgaagca tgcttctcaa ttaatgcaat taatcgttct tgttcagtca
     2581 atctcgttcc ggtaataaaa ctacaattca ataaacttgt catcagtatc cttagactta
     2641 atcttcgttt ttttgaaaag ctggattcca aagagattgt aattgctaca ctacatttta
     2701 aactagttaa ttcacttgtt tacaatacac aaccattgcc taatttgttc agtactaggg
     2761 gatatctttt ctggaagcgg atagtccgga ttaatgcaga gtccaaaaca atttatattt
     2821 ttccccattt caacccttcg ttgaaatagt cttaaaattc cgattaaaag aatataaagc
     2881 cgtgcacagt gcatcacaaa ttaatttagt aatgccatga gatttttaaa gaaaacaatc
     2941 tcgcaatggg atattataaa cttggcaatt tgtggtttat tttcattaga ttcattccgg
     3001 tgaatgcaat ttaaactatt gtttgttgtc attttgtttt ctgctacatc ttgggttaag
     3061 acatgccaga accaccggca tgtcttaacc ctgtcaattt cattgctcag gagaaatcat
     3121 cttttccggt ttcacaaacg cagcaaattg ctcggcggtt aaaattccca gtttcacggc
     3181 ggcttcttgc aatgaaatat tgtcatgatg agcggttttg gcgattttag ccgctttatc
     3241 atagccaatg tgctggttaa gagcagtaac cagcatgagg gagtggtgta aatagtaatc
     3301 aattaccggc tgattaggct ctattccctc tgcgcaaaat tcctggaagg agtggcagct
     3361 gtcggcgagc aggtttaatg agtgcaatac gttaaatatg ataactggtt taaacacgtt
     3421 taattcaaaa ttgccttggc tgtcagcaat tccaacggct gtatcattac ccagtacctg
     3481 ggcgcaaacc atggtcatgg cttcgcattg tgtcgggttg actttgcctg gcatgatgga
     3541 tgacccgggc tcattttctg gaataatcag ctcaccaatc ccgcaacgag gtcctgatgc
     3601 gagccagcgt atgtcattgg cgattttcat taatgcgcag gcgagagttt tcatggcacc
     3661 gtgcgcatgc accaaaggtt cgtgagaagc cagagcggca aatttgttag gggcagtcac
     3721 aaaaggcagt ttggtgattt tagctatatg ttcggcgact ttgactgcga attgcggatg
     3781 ggtgttcaat ccggttccaa cagcagtgcc cccggcagcc agctcatata attcaggcaa
     3841 tacctcttcc aggcggtgaa tgcacgcatc cagttgggca acataacctg aaaactcttg
     3901 ccccaaagtc agtggaaccg catcttgcaa gtgggttctg ccaattttta tgatgttttt
     3961 gaatgcgtcc attttcgctg cgaaaacctg gcgtaaattg cgtacagcag gtagcaattt
     4021 ttcgttaaac gctatggccg ctgcaatatg catggctgtt gggaaggtgt cattcgatga
     4081 ttgagacata ttgacgtgat cattgggatg aataggggac ttgcttccca gggtgccgcc
     4141 agccagctca atggcccgat tggaaatgac ttcattggcg ttcatattcg attgtgtgcc
     4201 gcttcccgtt tgccagacat gcaagggaaa atgctcatcc aatagccctt tgctcacttc
     4261 ttctgctgcc tgaacaatca aatccgcttt gtcttttggt aatttgccta attcaagatt
     4321 ggtcagggcg gctgcctttt tcaggatgcc aaaggcatgg gttacttcgc gtggcataat
     4381 gtctttgcct atattgaagt gatgcaatga acgttcggtt tgggcacccc aatatttatc
     4441 agacgggact tcaatttcac ccatgctgtc tgtttctaca cgtgttttag ccatgttatc
     4501 aattccttag caaagttaat ctgttgatta cgtttattta tgtctagctg ctttatcagc
     4561 gagttgggaa agaataaaat caatcgccaa aattctagca tagcagacgt aaattggtaa
     4621 tttttgttat agtcagggct tacgaaaaac gccaagatca aggcaaaaaa tattttttac
     4681 aaagagattg gtgtttttcg taagtcctga tagtgtcatt tatcgaaatg aggaatcatt
     4741 ggttgtgaga acaatacgta tctatcaacc cggcgaatac cagccggggc aagttctgga
     4801 attatcgcct gaggcggggc agcatgttgg tgtagtcttg cgcatggagc aaggtgaaca
     4861 actgactctg tttaatgggg acaatcaaga gtttacagca ttcattgaga gggtcaaaaa
     4921 aaagcaggta ttcgttagaa ttgcctctgt tcttgaggtg aacagggaat ccccattaaa
     4981 gatacatttg gctcaagcca tttccaaggg cgagcggatg gaagtggtga tgcaaaaatc
     5041 ggcagagtta ggagtggcat gcattacacc tatcattacc gagcgttgcc aggttaaaat
     5101 agacaaagaa aaaatggcaa aaaaaatgca ccaatggctt aacattatca ttggagcttg
     5161 cgagcaatgt gggcgtaatc aaattccaga gttgcgacag ccagtttatt tggatcaatt
     5221 tgtcagagaa gccaaagaac atctcaagct cattttgcat ccggcattta gcaagacatg
     5281 gcgcgattat cctgtccaac ctcctgatgt tgccttgatt attggcccgg aaggtggttt
     5341 cagtgatgag gaaattacat tgaccagtgg ctatggattt ctgcctttgt cactggggcc
     5401 gcgagtattg cgcacggaaa ccgcggcaat aactgcgcta agtgttttgc aagctgccgg
     5461 aggtgatcta taatagactt ctttccaact tggtataagc tgcgtctttt gttttatagc
     5521 tctattacaa gtatttctgg agtattatat gtaaactact ctttttgaaa atcttgtgtt
     5581 tgctctaaaa caaaacacac aggatacaca ggaaagattt tggatcgaat agcataatct
     5641 ggtcttggac cagaccttta tatttctaat aactgaggtt tatttatgag tgatcatatt
     5701 aaaacagtaa ccgatgcaag ctttgagcag gatgtaatta attcatccaa gcctgttctt
     5761 gttgattttt gggctgagtg gtgtggacca tgtcgtgcat tgacacctat tctggaagaa
     5821 gtggcagcca ctcatagcaa ggatatgacg tttgctaaaa tcaatatcga tgaaaatcct
     5881 caagctccag ctaaatttgg tgtgatgagt atcccaactt tgattctttt taaaaatggt
     5941 caggttgaag cagttaaaat gggtttgctg tctaaatccc aattaagtgc ttttgttgaa
     6001 agtaatctgt aacggtatta aagataaaaa gccctgagat gaaaatttat ttacagggct
     6061 tttacttttt tatggggctt gtgttaaaat taacctactt aaagggattt tttcttaaag
     6121 atttaaaagt taaaatcaag aaagtcaaaa taacttttta tttttagttt aaaaatgttg
     6181 gatcttgttg taaagctgtg atagcctgta gctatatgtg acatacttta aaacaagtga
     6241 gccacatagg cagcagattc cttagctgct attccctaac ataatttttt cttcggatat
     6301 tcaattcatt taaacaacaa atcaagtgag aagtatgaat cttagtgaac ttaagcaatt
     6361 acctattgcc gatctcgtta atatcgctca agagatgggt gtggaaaata cctcccgtat
     6421 gcgcaagcaa gacatcattt tcgccatcct taaagcccat gctttaaaag gggaagacat
     6481 ccatggcgat ggtgttttgg aagttttgac tgatggtttt ggtttcttgc gttccgctga
     6541 tggctcctat ctggcaggcc ctgatgacat ttacgtctct cccagtcaaa tcagacgttt
     6601 cggtcttcgt tcgggggata ctatttctgg taaaatccgc ccccccaaag acagcgaacg
     6661 ctattttgct ttattaaaag ttgatcaaat taactacgat acaccggata gcgcaaagcg
     6721 taagatttta tttgaaaact taactccctt gtttgctacc gagcgcctgg ttatggagca
     6781 aggtaatggc agcacagaag atctcacggc aagagtagtt gatttatgcg ctcctttcgg
     6841 ccgcggacag cgtggtttga tcgtgtcacc tccgaaagca ggtaagacat tgatgttgca
     6901 aaacattgct cgctctatag aaaaaaatta tcctgaatgt tatctcattg ttttgctgat
     6961 tgatgaacgt cctgaagaag taacggaaat gcaacgttct gtcaaagggg aagttgttgc
     7021 gagtactttt gacgagcccg ccaatcgcca tgtccaggtt gccgaaatgg taatagaaaa
     7081 agcaaaacgt ctggttgagc ataaacgtga tgtggttatt ctgctggatt cgatcactcg
     7141 cctggccaga gcttacaata cggttgtacc ggcatcaggt aaagtactga ctggtggtgt
     7201 tgatgcgaat gccctgcaaa gacccaagag attgtatggt gcggcacgta acatagaaga
     7261 gggcggcagt ttaactatta ttgcaacggc tctggtagac acgggttcca aaatggacga
     7321 agtgatttac gaagagttca aaggtacagg taacatggaa atccacttga gtcgtaatat
     7381 tgccgagaga agggttttcc ctgcgattaa cattaatcgc tctggtactc gccgtgaaga
     7441 tctcctgttg agtcccgaag aattacagcg cacctggata ttgcgtaaaa tcctgcaatc
     7501 catggatgaa tgtgatgcga ttgaattctt attggaacgc atgaagaatc ataagacaaa
     7561 tgccgaattc tttgatgcaa tgaagcgtca ggaataattt aaatgattaa ttaaccccgt
     7621 aattagcgga gcataattcg gggttcaaat tcgccttatt ttcaggttta taattcatgc
     7681 tcatagcctg tattaagctg tgctggtacg ggtttacagt aagcttcttt ttgagataat
     7741 taatgaagta ttcagatctg agagatttca tagcccaact tgaatcacgt gaattattaa
     7801 aacgtattga ttatcctgta tcacctcatc ttgagatgac cgtagtcagc gataaagtgt
     7861 tgcgctcagg agggccagct cttctgttta ccaatacccc caattacaac atgcctgtac
     7921 tgaccaatct ttttggcacg gtagagcgcg tggctttggg aatgggtgag gaatcaatag
     7981 tggctttgag ggagattgga aaattattgg ctgctttaaa ggaacccgat cctcccaaag
     8041 gcttcaaaga cgcttttagc aagttgccct tattgaagca agcgctgaat atggcaccca
     8101 aatatgtcag tggagccgag tgccagactc atgtgtggga aaaggatgaa gtggatttaa
     8161 cgttattgcc catccaaacg tgttggcccg gagatgttgc tcctctaatt acctggggtt
     8221 tggttactac tcgtggccca caccagtcca gagaaaacat gggcatctat cgccagcaac
     8281 tattaagtaa aaacaaattg atcatgcgct ggttatctca ccgcggaggt gctttggatt
     8341 accaggcctg gcaacaagaa tatccccaag aacgtttccc tgttgcggtg actttaggcg
     8401 ctgatccagc caccatactg gcagcagtta ctcccgttcc tgatactttg tctgaatacg
     8461 cttttgcggg cttgcttaga ggacaacgaa cccggttgac tcgatgcatt ggcaatgatt
     8521 tgcatgttcc agccagcgca gaaattgttt tggaaggtta cctggagcca ggaaatgagg
     8581 cacccgaagg gccttatggc gatcacaccg gttattataa tgaagtccaa tcttttcctg
     8641 tttttacggt agagcgtatt actcatcgcg ataaacctat ttaccacagt acttataccg
     8701 gaagaccgcc agatgagcca gccattttgg gggttgcctt aaatgaagtg ttcattccct
     8761 tgttgcaaaa acaattccca gagattgtgg atttttattt gccgccagaa ggatgctctt
     8821 atcgtttggc tgtagtcact ataaaaaagc aatatccagg acatgctaaa cgtattatga
     8881 tggctgtttg gtctttcttg cgccagttta tgtataccaa gttcgtcatt gtttgtgatg
     8941 atgatgtgga cgcgcgcaat tggcaagatg tcatatgggc aatgacgaca cgcatggatc
     9001 cgtcccgcga tacagtcatg gtagaaaata cacccattga ttatctggat ttcgcttccc
     9061 cagtttcagg attgggttcc aagatgggca tggatgctac cagtaaatgg ccaggagaaa
     9121 cacaaagaga atggggtaaa ccaattacaa tggatgaaga tgtgcttaat agagtaaatg
     9181 gttattggtc cttattagga ttaaaataat gacagataaa ataattcaag ctcaggtgga
     9241 ggacatttcc cctttaacgg acagcatcgt gcgattggtc ttaatgcctg atgaatacgt
     9301 tgattatcaa gccggacagt atttgcaaat cctgtttgca aatgatgcgt ttagttattc
     9361 aattgcgaat gcgcctttag gttctcatcg ttatgaattg catattcgcc atagccagga
     9421 taatgattat catcaatcgt tattggctca tatcaaacaa tatggatctc tgcgcttaag
     9481 gttacctttg ggtgaatgca gtatcgatca attacacccc caaaaaccca ttttattcat
     9541 tgccggcggg actggatttg cgccaattaa agccatgatt gagcaattac tcgcagattc
     9601 ggacagcagg ccatttgagt tattttgggg agcacgctca caaagcgatt tatacatgga
     9661 tgaaaaagta aggagttggg gctctcatgc cagccacttc caatattttt cgctattatc
     9721 cgataaaagt gaggatactt tggcttctcg agttcttgcc aggcatgcaa acattctacg
     9781 ggactggcaa attgtcatca gcggtccttt cgatatggtg tacagtactc gtgatacctt
     9841 ggttggttac ggcatatcgc ctacccagat gttttcagat gcctttagtt ttgaatccaa
     9901 aacagatgat gcaaaaaact gagtggaagg agtgaattaa tggaaacttt atatcaaatt
     9961 ttaggactca ttggcgccgg attgattatt tttatattgt accgatttat taagggaagc
    10021 ccagagcaat ttagcaagga aaatatgagc aaaagcttta tgacgatggg ggttttaggt
    10081 ctgattttaa taggttttat tgccttgctg gtgttgatgt tgaggaatac ataaccagct
    10141 ccatgccctg ttggcatctc cacctcataa gctagtgaga cgggatagct ttctgataac
    10201 tgcccttgtt tttaattaaa taatcatcaa tattggggtt gttgataggc tcctgactcg
    10261 tatcaggaag taactggcat tcttcgacat aagttatttg gctgctgtca tcaactaaca
    10321 ggcggtacca tgggtttctt gttgcgaatt catgctgagt tgcccgggga ttgtatttcc
    10381 ctgaggcttg gaatatggga tctacatcaa taacaatcgc ccggtatctg atgcgtttat
    10441 gaataaccag atcaccgata ttgtatttgg ctattttatt cataatgggc tccgtttttg
    10501 agcattatga caaatatatc ggtaattccg cttttaactt gagcttactg aattaaagtg
    10561 ggcgcggggg atttcccata aatcctggct aaaataatcc ccgcttcata aagcaaacac
    10621 aaaggcaagg ctaatgcaac ttgagaaaaa acatcagggg gcgtgagtag cataccaata
    10681 ataaatgccc caactatcac ataaggcctg atttttataa gtgtctcaac cgtagtgaaa
    10741 tgggttttta ccagtacaag acaaattagt ggaacctgaa aagaaaaacc aaacaaaagc
    10801 agcatgcgtg taatgaaatc tatggcgtaa gccatatccg gcatgagtcg cactccttcc
    10861 ggaagggcct gagcaaaaaa ctggaacata taagggagga caagataaaa gcaaaatagt
    10921 gtcccaaaga caaagagcaa aagacttaaa atgatggtgc ctctgatttg ttcctgctcg
    10981 tttttataaa gccccggacg cataaaacgc caaagttgca ataaagcaaa cggagcagtg
    11041 agcaacaaag cggtatccac tgcaagtttt aaaggggtga aaaccgagga agtaatctgt
    11101 gtggcaatca agccctcttg attcggtaaa gaacgcaata acggccttac caggagatga
    11161 aacagttggt ctgatatgaa aaaaaacaat agcgctaaag cgccaaacca gagaagggta
    11221 tagattgctc tttgccgtaa ttcaattaaa tgaattaaca taaagctcat tggagttgaa
    11281 tgccgagatc atagaataaa ccattccatt tgatttttct aattttgatc attgcctgac
    11341 acagtagcat atgaacccat tgctatgggt tcaattcaag ccgggtaagg aattctactt
    11401 ttaacaccga ttctttcagg tcggatttct ttcagacgat tgaattgatc aacaaaaaca
    11461 acagtgggtt catgtgctgc acaatcctct tccagaattt gcgtaaaaga ggcgataata
    11521 accaaatcgc caggggttac caaatgagct gccgctccat tgacacagat atccgttgaa
    11581 cccttttcac ctgtaatggc ataagtttca aatcgggttc cggcagtgat attccagaca
    11641 ttaaccgctt catagggcaa tatgtttgcc acttttaaaa gctctggtga aatggtgata
    11701 ctcccttcat aatccagatc cgcctgtgtg acgcaagcac gatgaatttt tgatttaagc
    11761 atttttctat aagccattat ttacttccta aatttaagcc tggaaaaagc aattggctaa
    11821 atacgactga gtttatttgt tacatggctt tttccagatt gaaataacta aaaaaaatag
    11881 ataagaatat caaccttggt cattgtggtt gaattttacc ccataccttg gagtttacgc
    11941 tatataaatt tttaaaattg agggagaagg ctttgagtta ttgcgtattg tcggggtttg
    12001 ctggcacttg acactaacct acggtaaaat taacaggctt ccccaatggg gtataccttg
    12061 tgatgtcaac aagattactg aattaatttt atcctaacat aggtgataga aagtgaggca
    12121 tagcccacgt aaacgttttg gtcaaaattt cttacaagac aaatatatta ttaatgagat
    12181 tctacgcgct atcaatcctt tggcggatga taatatgctg gagattggcc ctggtttagg
    12241 cgctttaact cagccattat tgcaaaaact gaatcgatta acagccattg aaatcgatac
    12301 ggatttacaa aactatttaa cccgcttgcc cgtatctcaa ggcaaactga atttaattcc
    12361 tgctgatgcg ttaaccgttg acttttgtca attcggcccc catttaagag tagtgggtaa
    12421 tttaccttat aatatttcta ctccattgct gatctattta ttgaaattta taacttgtat
    12481 tgatgacatg cattttatgt tacaaaaaga agttgtagaa cgaatagcag caacccacgg
    12541 gactaaagcc tatgggcgac taagcgtgat gttgcagtat cattgtgagg tagagtattt
    12601 atttgatgtt cctccagaag cgtttgagcc caagcccaag gtagactcag caatagttcg
    12661 attaacacct tatcgtgttt cgccatttga gtctgtaaac actgagaaac tggaaaatat
    12721 tgttgcgaaa gcatttgcta tgagacgcaa aactcttact aataatttga aagggattat
    12781 ctcattatct caactcaatg atttaggtat tgatggaggt aagcggccag aacaaatttc
    12841 ggtggcggaa tatgtccaac tagcgaaatt tatttccaat tagtgtaaaa ttatatgata
    12901 agttattgtg gaggttgtct tgtttcatcg tcagcgtaaa agctctttat ctgctcagtc
    12961 caagtcatta aaagatttaa tccgcatggt gaccgcagag cagtttctga gtgaagagaa
    13021 acgtcaggta ttactccaga aaatgagagc atttagcggg ttggaagcat ctcgctatga
    13081 gagtttatgt tctgttcttg tcgagaatct ggtgaactat tgtcaaaatt tgccggaaac
    13141 cgcgaacagt tattattccc agcccggtgg tttggtcgat catgctttaa acagaacaga
    13201 ggctgcattg agtctattca aggagtttac cgttcaagaa cagccagatt tgctttccga
    13261 ggaacagaag ctttggcaat atgctcttta ttcagcggca attttacaag gccttggcaa
    13321 attatttgat gagtttcgca ttaatttgtt tgatagcaac ggtcaattgt taaaacaatg
    13381 gaaccctcta ttagaaagct taatccaaac tggaagttat tatgattatc agtttgagaa
    13441 agaagcggac gcggagttcc gccgccggct gaacttgttg ctggccagaa ctttgatgcc
    13501 ggccagtggt tttgcctgga ttgcctccaa tccggaagta ttggctgttt ggttagcttt
    13561 actgaatgaa gattatcgtt ctgctggtac tttgggcgct attttaattc gagctgatgc
    13621 cattgcgata cagcgctatt ttctggcgtt tatggcacga agtggggcaa gacgaagtgg
    13681 ccgttatggc agagcggtaa cttttaccgg cggtgtaccc gaatccataa cagaaaagga
    13741 acaggcaata ggggtcgaat tcatccaatg gcttatcaag tcgttggatg aaggtcgaat
    13801 tatggtgaac aaggccccat tgtttatggt tcctggcggc atgttgatgt gccctgaaat
    13861 gtttcaattg tttgttcgtg agcatcctga gtataaaaat tggcaagcca tacagaatgg
    13921 gtttttatca ttaggacttc atggccggga tgcggggggc aatgtaacca gtcgttttga
    13981 acaggctcat actcagcaaa tgtataccgg agtcgttttt gcaggatatg gggttgcttt
    14041 gcccaactct gtccaggtgc atcatttgaa tactggcaaa atagaatcca tgtcagcact
    14101 ggagttaatt cataaatcac aatacaccag ccaattcaca cagcaatcaa gtgcgattgc
    14161 aatagcgcct ttgcaacgat tggcagcaaa tggccaatgg cagaatattg agcaggatat
    14221 gtcccaattg acatttggag caaaacgcgg tgcctgatta tgcaataggg gatgtacaag
    14281 gttgttatga tcctttacaa cgtttgcttg agctaatcga ttttaacgag aaggaagatt
    14341 gcttgtggtt tgttggggac ttggtaaacc gcggtcctga ttcattggct gtattacggt
    14401 ttatttattc cttacctgtg aaacccaaaa taaccttggg taatcatgat ttgcatttat
    14461 taggcttatt attcggcggg caaccctgga aagggcatga tgatacactt gaagaggtga
    14521 tgctggctga tgatggagag gagcttgggc attggttgcg caagcaatcc cttctttgca
    14581 ggtcatctga gttaaacata gtcatgtgtc atgcaggtat tgctccttta tgggatttat
    14641 cgaaggccgt gggcttggct aatgaattgg aagcggtgct ttcaggcgat tcatatcacg
    14701 aatttttcgc ccagatgtac ggcaataagc cggatatttg gtcagatgat ttggttggac
    14761 tggataggtt acgagtcatt accaattatt ttacccggat gcgctattgt gatgcccatg
    14821 gacgattaga tttgggttat aaggggactt tatccaaagc ccccaatcat ctgtatccct
    14881 ggtttgaagt gccatgccga aaagaaattg aaatggatat cgtttttggt cattgggcgg
    14941 cgctcatggg gcgaagttct catccgcgaa ttcatgcgat agataccgga tgtctttggg
    15001 gagggcaatt aacggcatta cgcctgcagg acaggcaacg cttttcggta cccgggtatg
    15061 gggttagtcg tttcgaataa ccttgaggga taggcatttt tataaacaca ggcgataaca
    15121 acggtgaaat ttttaaataa aagcacagca ttgttaccgg taattatttt atcgggcctg
    15181 attagttttc atgccgcaag cgccaagact tcttcaaatg ctgataattc actttattcc
    15241 tggacagaaa atccttcagc tgaagcacag agagtggagc aagcggagga aagactaaag
    15301 gcagctattg ccaacccgga atacatgctg aaaaattgga tagagctgcc tacctcgcct
    15361 gaatcaaaaa agaaaacaag gcaccagttg acgataagag aagccatact gttggccttg
    15421 cgttataatc ctaatatcca gaatgcggaa ttagatcgta ttattcagcg ctatcaattg
    15481 cgtttggcta ataatgagtt tgaattgcaa tatgctcttg gtgcttcagg cgttgtgcaa
    15541 aaaagtactt ttgaggggat aggcagttct acgaatcatt cttttctggc cagtcctgaa
    15601 ttggatttga aaaccaaaat aggcaccaag gcgtctgtat ccatcgataa caatgtttcc
    15661 accaacaaca gctataatcc ggtattgaat ttatccatta ctcagccttt gctccgtgga
    15721 tttggcaagt cagcgaatga agcaaaactg ttggatgcga ttgataagga atggctaaat
    15781 aaattaagtt tacagcaatc ggttatggat cagattacgc aggtgattac ggcgtacagg
    15841 gctttgattt taagtgggaa caatttagag aatcagcgct tgcaattgga agaagccaga
    15901 aaatcctatc gtattaatga aaaaaagatt gcagcgggac aattagaacc taccggaaat
    15961 attcagcaat cctaccagat agaatctttg agtttgatgg tagaacaagg tgaaaatgat
    16021 tttaaattag cctctcagga attattgcaa atgattgggc ttgatccgga tttgcatatt
    16081 tcagttccca gtgatgtgac gataaaggaa attatagtgc ctgacttgca agagtccatt
    16141 gccatcgcat taagtcacaa tactcagtat ctggcccaga aaatggcctt gcgtgcggat
    16201 gagagggctt atactgttgc aaaaaatcag caactttggc agcttgattt ggcgggtact
    16261 gtgcaaggcg gcgcagtcaa tgatgtcacc gggaccagca gtggtctaag cagcatttac
    16321 aatgggaata atatcacgga atcagcccgg gtcaccctga ccattccatt aaatgacatt
    16381 ggccggcgta gtcaattaat taatgctaaa gtccagttgg aaaaagacaa aatcaatctt
    16441 atcgcagcca aaagagcttt aattaccagt gtaaccaata ccatcaataa tattcaaagc
    16501 ttggccaagc gctatgagct ggcacaaaaa caagtcaaat tggcagagca gtcttacgct
    16561 ttggaaaaga aaaaacagca ggcaggaata tcgagcgccc tggatgtgaa caatactcag
    16621 aatcaattga ttcaggcaca gtcaggattg ataggggcaa aaattgcgta tctcaatcag
    16681 ttatccgcgt tgcaaaggat tttagggacc acgctggatt actggcagat taaactaagg
    16741 tatggcggat gaacaattat ctgaaaatat tctctatggt gtcattgatt ttaatgctgg
    16801 tgtcttgtgg cagttctgat aagccgcaaa cctcttcatt aaaaacgtat gaagtgaaac
    16861 ctcaaccttt acacaaggct ttgcatttta ccgggaccgt gcaacccata agggaaagca
    16921 gcctgaccag tccaatggaa gccgttgttg aaaccatgca tttccattat ggccagatgg
    16981 ttaaaaaggg agagattgtt ttaactttaa attccaatga attgcaaaaa cagtataacg
    17041 acacattaac ggattactta aaggcgaaag acagttattc cattgccaag gcaaaatttg
    17101 taggaaccga ggagctgtgg aatgccgggt taatctccaa aaacaattac ttaagtgaaa
    17161 aatccgggtt ggatacagcg cgagtcactt taatgcaggc cactcgcaag ctttctgaaa
    17221 tgttggaaaa aatggatgat aaaaacactc agaatatatc caatctcagt ctggctgatt
    17281 ttgacaaagt gaggaagatt cttacctcaa atcataattt gattcattta aaggctccca
    17341 gtgatgggat catgctgtac cctcccaaat caggagaaga taaaaacaat cgagtgaccg
    17401 taggatcgac cataaagtca gggcaagtca ttgccttgat aggcgatttg aatggaatca
    17461 gtgtggaaat tgatgtccca gagatagaca ttgataaaat tcgcccggga atggaggcaa
    17521 caattagtgg cgttgcattt ggcaggcatc aactgaaagg taaattggtg gcggtgaatg
    17581 cgcaggcatc gaataccagc acgggaggtt taccctcctt taccgctgtc gtggaggtca
    17641 attccctaac tcctgagcaa cagtcatgga ttaaagtggg gatgagtgcc tccatagagc
    17701 tgaatgtgga gtcgaataat caattattga tccctatcgc ggcgattaaa agggaaaaag
    17761 gcaacagcgt tgttcaggtc caattagcca aggggaaagt ccagaaacga atgattacta
    17821 ccggtgccgc tcaggcggat tcagtagtgg ttgaaagtgg ccttaaatca ggagatgtcg
    17881 tggtttatga ttgacagacc tttaatccaa ctttctgatt tggtgaagac ttatcatctt
    17941 gagggtatca gtaccacggt attaaaagca gtgtctttga ccgtaaacga aggtgatttg
    18001 ctcgctattg tcggtgcgtc cggctctggt aaatccacgt taatgaacat catcggctta
    18061 ttggataaac cggatgcagg aacttatacc ttaaataata gaaatgtggc cagcttaagc
    18121 gacgatgagg cggctgattt gcgcaatcaa aatataggtt ttgtttttca gcagtttaat
    18181 ttgcttcctc gttttacagc aatgcaaaat gtggctttgc ctttgacgta ccgaggggtc
    18241 aaccccactt tgataaaaga aaaagtggag caagcactgg ataaagtagg aatgcgccaa
    18301 tacatccgcc acaggccaac ccaattatct ggcggtcagc agcagcgagt tgctatcgcc
    18361 agagcgttag tgaccgaccc acaggttatt ctcgctgatg agccgaccgg tgcgctggat
    18421 tcaagaacag gtagcgaggt aatgaatttg tttttggcct tacaccagga aggccggact
    18481 atcatcatgg tgacccatga tgagcacgtg gccgcgcaat gcaaacgccg aataacctta
    18541 gctgatgggg caattatagc ggagtccggg caatgaattt tcttaatcat tgtcaacaag
    18601 ccttggttaa tttaacagcc tccaagctcc gatcgctttt ggctgtgctg ggaatactgg
    18661 tagggactgc cgctgtcgtt gccttgatta gctgcggaca actggctacc gagaaggcgt
    18721 tggagcaatt caaggcattg ggtaccgatt tgctggctgt ttccgtgtat caaaagatgc
    18781 ccagcaaatc ccatagtgaa tccgactcgt taacaataga tcaatggcag cagatagccg
    18841 agcgtatacc ttatgtctta agaattgcgc cttacagcac ggcttaccaa actttaagct
    18901 ttaatggcaa agtgatgcag ggggcggtga ttggcgccga tgaaagcctt gctgatatca
    18961 ttcatgtgac cttggcacag gggcattttg tttccttttt ggattctttt gagcattttt
    19021 gtgttattgg cgatagcctg gcacaacaaa tcaaagaaat taccctggat gatcccattg
    19081 gcaagcaatt acgcataggc caatccttgt ataccattat cggcgttgcc gagcgttgga
    19141 aagaaaatgg cttttttaat gaggacatta atcaggctgt cattattccc ctggctggaa
    19201 tgagcctggt cagcaaggac gccaaaatca ataatgcggt attgcttctc aagcccgaca
    19261 gccccattga tgaggtcatt aatgaaataa aggaaatcat taattctcag gcgccgaagc
    19321 tcagtgtttt tccgcgcagc gccaaacaaa ttatagccag catggaaaat cagggtcgta
    19381 tttttacctt gctgttagcg gtcattggtg gaatatcctt gctggttggt ggcatcggtg
    19441 tcatgaatgt gatgctggtt tcggtcagcg agcgcaaaaa agaaattggg attcgcaaag
    19501 cagtgggtgc gaaaaacaga gaaatccagg ccttgttttt ggtggagtct gtcatgctgt
    19561 ctttgctagg gggtgtgctc ggagttattt taggattgat ttttaccaga atagtcgctt
    19621 atttcagtga ctggactttt actatctact tacttccccc aattgccggc tttctggttt
    19681 ctgctgccac tggaatattt ttcggatttt atccggctcg acgggcttcc aagctggaac
    19741 ccatggtttc tttacggagt gaatagcttt gtacaatctt gccatcaaga catggtttgc
    19801 catggattta cgctattgac cacatcatgg atttccgtgg acgcttaatt taagtacaat
    19861 actggacact ggactaggca cttggactta aaatcagcca aagtgccaca aaaacatcag
    19921 ggtatttttt tgccctatgc attaaatggg cttttataga ggctaatttt taataaatga
    19981 gctatctttt aagaatacca gtatgattta aacggattgt tttttataac aattaaagag
    20041 aaataaaagc tgggcgaaat acatattatc aaacaataaa tataactgag gggaagagct
    20101 atgaaaaagt gggggctcgc tattgttgca ctgatgttat ttaaccaaat aacctatgca
    20161 agtcaaagct ggaatcaatg ggttgccggg gtgcgtgagg aagccttaca acaagggatt
    20221 ccacccagtt tatttgatga agcctttgct ggcattcatg aaccaagccg gaaaataaaa
    20281 ggttttttac gctctcaacc tgaacacagg ttaacgttca tgaaatatcg taataccaga
    20341 gcagacaatt accgaattgt gattggaaga aaagagtaca agagaaacaa ggaaatactg
    20401 gaagagattg cccaacaata cggagttaat ccttgcttta ttgtgtcgtt ttggggtatg
    20461 gaaaccagtt acggctccta catgggaaat ttcccggtaa tcaatgcgct ggctactttg
    20521 gcctatgatt caaaccgtaa agactttttc cgcaaggaat tgttcctcgc tttacacatc
    20581 ctgaaaggcg ggcatgtcag cctggctgat ttcaaaggag aatgggctgg cgcctctggt
    20641 caacctcaat ttttaccatc cagctgggtc aaatatgcgg tcgactacga tggggatggc
    20701 cgaaaagaca tatggaagag caagcccgac gttttcgcat ccatagcgaa ttacatgaaa
    20761 caaaacggct ggcaggccgg agagccctgg gctattcaag tgaaactccc tgccaagttt
    20821 gatatgaaac tggaagggaa aggcatagtc aaaccagtca gcgagtggaa tgcattaggt
    20881 gtgcgcacgg aaagcggcga acccctgccc tatcaaaatt tgcaggccag tatcgtacag
    20941 ccttacggtg gcccgacctt cctggcgttt cccaactaca agatgatcct gcgttataat
    21001 aactccattt attatgccgg ggctatcggg tatatggctg ataaaatttg ccagggtcaa
    21061 tgattcgcct caattatttg ctggagacat aaatatctat ggatgaattc cactgggcca
    21121 ggcgttcagt ggagttcaca tacgccaagc tttataaatc ataggtaacg tgaattatgg
    21181 ataaaatcag ttgttatctc gaaccgctcg ggctcaggag gcatttatgc catctcgaag
    21241 cctagtatcg gattttatgt ttcatgccaa ggcttcgaga cgatgcttag gcatctcctc
    21301 agcccgaacg taccctgggt atcgacgggc tgttatatcc tctttatcca taactcatgt
    21361 taggtattaa aatgaatcag aaatcctttc agtggtcagt tgttggtgcg gggcctgccg
    21421 gaattgctgc agtaggtaaa ctattggatt atgggatcgc gccttctgaa atattatgga
    21481 ttgatcctca ttttaaagtg ggtgattttg gacagctctg gcggaacgtt tccagtaata
    21541 ctacggtcaa gctcttctca agttttctgc atgctgcaaa ctcattttcc tataaaaaag
    21601 ccccttccac atttcgttta aacaatctgg atcctgataa tacctgcctg ttgagtgatg
    21661 ttgcggagcc cttgcaatgg gttaccagcg ttctggtgaa tcaggttatt gcagaagaga
    21721 ctgtcatcca cagcttgtct ctctcaggcg gttgctggac tttatgctct gacacacaac
    21781 aataccgagc gaaaaatgtt attttagcga caggggctgt cccatccagc ctcaactacc
    21841 cgggtgttaa cgtggtaccg tttgaaattg caatcgataa acagcgtctt gcccattttg
    21901 ttgatacgaa tgagacttac ggagtttttg gctcttctca ttcggccatt atgattttac
    21961 gctctcttat cgaattaggg gtaaagaaag tgattaactt ttatcgctcc ccttgccgtt
    22021 atgccatcaa tatgggcaat tggattttat tcgataacac aggcttgaaa ggtgatacgg
    22081 ctgcttgggc caggaaacac atcgacggcg ttttgcctgc caatcttgtc cgctactacc
    22141 caagcgaatt aaatattgcg cgccatcttc cagagtgcga caaagtcatt tacgccgttg
    22201 gctttgaaag acgtaaaaat atgaccattg ataattatga acacgttact cctaatcctc
    22261 atgtgggcat tatagggcca ggcttattcg gcttgggcat agcctatcct gaactcagtg
    22321 tcgatccgtt tgggactatt gagcatcagg taggattatg gaagttcatg gtgtatttgt
    22381 gtaaagtgct gccggtctgg ttcaaatatc ctgcctagag ttgggaaatg ataattcaac
    22441 acatggccac tcttcatcgg ctggttttct acagactcgc aaaactctgt tatattgaca
    22501 tgcttaggaa acgccaagga taaaaaaatg acgaagatat taatttgttt aggactgttt
    22561 ttaagcagca atttactcat ggcgaaacca ttgtcgcaat tggactctgc taatttgctc
    22621 ccctgtttta acatagagca agcagaacgc attggaaaac aaatcaataa gctcttacag
    22681 catgagtttt gcgaggaaaa cagcaatcca aagaaatttg cctctatcag tcacaatatc
    22741 ctgcccaaaa ttatgacaga aacattttta ggagtaaccc cgccagaaaa ctggcagcaa
    22801 ttaagcgacg atattataaa aaactgcatc gcaaacaaga atctatgcaa aaaagcagct
    22861 cgcaaagagt tggaagaatg catcaaaccg agaattcctt tgatcctgat acaatttggc
    22921 ccctggcttg cccaaaattg tccacaattg aataagtctt tgattgaaca atggccaagt
    22981 aaacaaacca ttctcaaaaa gataattaat gaaaataaaa gtgtcgagta attgagacag
    23041 gtttaattcc gagagttatg cctgataaaa ccacatttat ttacctaatt tcatcaaata
    23101 taactcaccg atatgatcta caaccaggtt cttaaatgat gggtttgcgg acgtctaaaa
    23161 ttgatgttca aaattttgta atacatagca aaagccagtt tgaaaactct gcgcattaat
    23221 caaagttcat tttgatctat gtttttagcc tttctgaaca aaaaggaata ataatgagag
    23281 tagggtattc aagagtatca acgtatgaac aaaatttgga cttgcaattg gatgaattga
    23341 aaagggctgg gtgtgaagaa atttttacag acgtgattag tgaatctgtt tctgaaaggc
    23401 caggtctgga taaagttgaa attatctaag gaaaggagat gtcttagtcg tttggcgatt
    23461 agataggtta ggacggtctc ttaaacatct tatttcttat gtaagtaatc ttgaagaaaa
    23521 agtcattggc tttcatagca ttactgagtc gatcaatact gagacaactt ctggaaagtt
    23581 tatgttttat gtctttggag cactttctga gtttgaacgt aatttaatta aagagagaac
    23641 acttgcaggt cttggagctg ctagagctag agatcgccaa ggtggtaggc ctgaaaaact
    23701 tagtgatgag caaaaagagc tagtgaagac tctttacgcc aataaaaaac attcaattca
    23761 atcaatttgt aagatggttg gtgtgggaaa gactacactc tataagtata taaacgaata
    23821 aatgtaaaag tataaaaact taatgttaag acataaataa aggaataata atgttttatt
    23881 tatctttatg gggcgccatt actggtacga taggaactat tctaggaata atgaccctca
    23941 ttttaaaagc aaaggaattt aatagaaata aggcaaactt aatatgtaaa tctgag
//
LOCUS       DATNSN010000034        22312 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.034, whole genome shotgun sequence.
ACCESSION   DATNSN010000034 DATNSN010000000
VERSION     DATNSN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22312
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.034"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1602)
                     /locus_tag="VAH76_001120"
     CDS             complement(<1..1602)
                     /locus_tag="VAH76_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO1385764.1"
                     /translation="MKKNRFTYGLALGALGVVFGDIGTSPLYALKVTLSGIPINQFNI
                     LGVLSLIFWSLIIVVSFKYLMIIFRADNDGEGGILALLALMKHKSTKYQPLFYIVAIF
                     GAGLLLGDGMLTPAISVVSAVEGLGTLSDKLYPYVLPIASVILVLLFSLQAKGTARIG
                     YLFGPLILIWFITIAILGILQIVEHPVVLQAINPYYAIAFLVDEGLRGYFLLGGIFLV
                     VTGGEALFADIGHFGKNPIRFSWFFIALPCLLLNYFGQGANLIVRPEEISNPFFMIAP
                     PWFYLPLIIIATVATVIASQAVISATFSLTKQAVLLGLCPKIPIVQTSMLHSGQIYVP
                     QINFILFIGTMAFCLAFKTSDNLAHAYGIAVNLEMLLVDAMVAYAAVSIWRWSTFNVI
                     FLFGLFLLIDLAFLGANTHKFITGGWVPIVLAFVIAFIMYSWRYGLEYLRDNFYMNKE
                     DISKILKQLQYKSLNQLPGVSAIFITDVYDKSGGSFLHFLKLNRSVPENVLIVNYIVD
                     NIPYVHYSQRYEIVCLDEKVCKLVIH"
     gene            complement(1630..2613)
                     /locus_tag="VAH76_001121"
     CDS             complement(1630..2613)
                     /locus_tag="VAH76_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385765.1"
                     /translation="MRQFTKYLAIFLFSSSVSVAYAGPWFTGPLLAPAGHTVAKGHTN
                     LEVYGIDVFTDGQYNASGQRISTPKFKTFAANPILTHGFTDWLDVQLTVPYLFNSTRG
                     VHENHLADVSTALGIQVLEQKGSPKRIDFRVLLQETIPTGRYDQLNPALLGTDATGLG
                     SYQTLVGLDFQYLLQVFKTHYLRTRFIASRLFSSNVHVDGLSSYGGSINTHGTVKPGI
                     ENNLDLAFEFTLTQHWVAVMEGYIATGKATRFNGILNFNSIGSPPVNVSDLDAAPVVI
                     GNGSFREEALAPAIEYNFNENIGVIGGVWFPVSGVNTSHYMTYVLAINAFW"
     gene            2691..3863
                     /locus_tag="VAH76_001122"
     CDS             2691..3863
                     /locus_tag="VAH76_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO1385766.1"
                     /translation="MKAKVILVNGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIG
                     SEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEE
                     TNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVQVLQELFPHNHIIWL
                     PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKL
                     SRGKRVLDLYTFTGGFALHAAKAGALHVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKA
                     DAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNC
                     SSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV"
     gene            3988..5136
                     /locus_tag="VAH76_001123"
     CDS             3988..5136
                     /locus_tag="VAH76_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEO1385767.1"
                     /translation="MSLALKIKSIILSFFLVLMATVACATQVSLRDKIGQMLILGFQG
                     KVVDEKSPIAQAIEKNNIGGVILFDFNQQSQTFDKNIESPEQVNQLNNQLQTLTQKAN
                     RKYHRDNLPLLISVDYEGGNVNRLHPRYGFPEMPSAKDIGSMSLERADASAELMANTL
                     KSTGFNLNFFPELDVNINPDNPIIGKKDRSFSSIPEIVTQYAQLYTEQFIKHQIHCSY
                     KHFPGHGSSLSDSHLGFVDISDTWSEQELIPFLQLLSQPNHCDMVMIAHIVNRKLDMT
                     GLPASLSYQIINGLLRHDLKFNGVVITDDMQMKAITNYYGLETAVTLSINAGADMLIF
                     GNQLVEKFQDSTEIIDMIEQKVRSGEISEQRINEAYQRIVKMKRSFNY"
     gene            5277..6002
                     /locus_tag="VAH76_001124"
     CDS             5277..6002
                     /locus_tag="VAH76_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEO1385768.1"
                     /translation="MKNVLKREDAANKKLGVLIYENVQPMDVIGPWEVFATWKTILDA
                     PIDMFLVAETGNEVCCANQISLKPHCEFNNSPQFDYLLVPGGVGRREQVNNPRLISFL
                     KKQAQNTDYLLSVCTGAFLLHAAGLLIHHEATTYWRAMPELKSFEDVQLVEKRIVKSG
                     KVWTTGGISSGIDLAFEFISTIAGNDVAGKVQLLFEYFPECKLYSQPAMVSELPSYYA
                     TKKDEAVLEMNLPDYIKEYFKKS"
     gene            complement(6091..6714)
                     /locus_tag="VAH76_001125"
     CDS             complement(6091..6714)
                     /locus_tag="VAH76_001125"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEO1385769.1"
                     /translation="MSDLDINSLDWQKMNGLLPAIIQNAENGKVLMLGYMNQEALIAT
                     LTTGQLTLYSRSRKRLWRKGESSGNSMALQHISADCDNDSLLIQVIPKGPACHLGYST
                     CYQPHNNYTLGFLDGLIELINERAEEKNPNSYTAQLLDSGVNRCAQKLGEESVEAVIA
                     AVSNNKEELINEASDLIYHLLVLLKACELSFYDVLLCLQARDRSVHT"
     gene            complement(6711..7478)
                     /gene="hisF"
                     /locus_tag="VAH76_001126"
     CDS             complement(6711..7478)
                     /gene="hisF"
                     /locus_tag="VAH76_001126"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEO1385770.1"
                     /translation="MLTKRIIPCLDVLDNQVVKGVKFRNHRIIGDILPLAAHYSASGA
                     DELVFYDITASSDDRSVCPVWVNKVAATINIPFTVAGGIRTLHQARSILNAGADKISI
                     NSPALENPSLINELCKEFGKQCVVIGVDSQWIIDDYYVYQYTGNENKSTNSKRKTREW
                     IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVF
                     KKSHVSGALAASVFHDKILAINDIKLALQTENIEVRL"
     gene            complement(7472..8191)
                     /gene="hisA"
                     /locus_tag="VAH76_001127"
     CDS             complement(7472..8191)
                     /gene="hisA"
                     /locus_tag="VAH76_001127"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEO1385771.1"
                     /translation="MLVIPAIDLQSGRCVRLKQGRFDQVTQFSAFPIERALHFAKLGA
                     KRLHVVDLDGARSGKMQQLELICSMQKTGIPIQAGGGIRSIEQALECSNAGISQLVIG
                     SLAITNSDLTIQIIEKIKPENIVLALDVRVDTKIPLLAINGWQNNSTSSLWEVVSYYE
                     KYGIKNILCTDIACDGMMNGPNFDLYQQAVEYFPQIAWQASGGVRHMQDITTLNSLGI
                     SAVILGLMLYQDNVNFEELLC"
     gene            complement(8185..8784)
                     /gene="hisH"
                     /locus_tag="VAH76_001128"
     CDS             complement(8185..8784)
                     /gene="hisH"
                     /locus_tag="VAH76_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEO1385772.1"
                     /translation="MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILP
                     GVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGFAE
                     LLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
                     EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESTC"
     gene            complement(8781..9839)
                     /gene="hisB"
                     /locus_tag="VAH76_001129"
     CDS             complement(8781..9839)
                     /gene="hisB"
                     /locus_tag="VAH76_001129"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEO1385773.1"
                     /translation="MKKILFIDRDGTLVEEPFDFQVDSLDKIKLTSGVIPALLQLQKA
                     GFTFIMVSNQNGIGTVAFPEEDFAVCHEFILDLFSSQGILFDEIFICPHTPEDNCICR
                     KPKTGLLEPYLKETAFAKQYSWVIGDRDTDKEFADNLGVNFLPISKTHTWEMVASAII
                     NDARKASVQRKTKETAIDLSVQLDSDQTSVINTPIPFFTHMLEQVAKHGGFDLRLQAS
                     GDLEVDEHHLIEDTAIALGEAIRTALGDKWGINRYGYTLPMDESLACVAIDISGRSFC
                     DFKGQFTREFVGGMATEMVPHFFQSLSSALGATIHIEVTGTNHHHMIEACFKVLGRAL
                     RQACSRTNNYLPSTKGVL"
     gene            complement(9817..10911)
                     /gene="hisC"
                     /locus_tag="VAH76_001130"
     CDS             complement(9817..10911)
                     /gene="hisC"
                     /locus_tag="VAH76_001130"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEO1385774.1"
                     /translation="MSILNLVRADLLNSQNYVPGGESARYRSHANELPWSPVTMGEHN
                     LNYYPNIGLQIELQKQLAKRYQINSDQIILTRGSDDGIDLTTRLFLTAGKDAFMQFPP
                     TFPMYAFYVRLQQAELIECPLDRRTNFRLTLDQIENSWKPNCKVIMFCSPNNPTGNLV
                     DLNLIAKTCELYANQSIIVVDEAYIEFANAPSATSLIGEFENLIVLRTLSKAFGLAGL
                     RLGCIIAQSPIIQAFNKIIAPYSIATPSMELAKRALNNSDWFTKAIEQIKSSRAWVIK
                     KFTDNPIIQKIYPTETNFILIQTRFSKQLTTWLAHYGIAVRDFPSSSLLHDHLRITVG
                     HDEQNQLLINALSSFNADVAGLNYEKDFIY"
     gene            complement(10912..12207)
                     /gene="hisD"
                     /locus_tag="VAH76_001131"
     CDS             complement(10912..12207)
                     /gene="hisD"
                     /locus_tag="VAH76_001131"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEO1385775.1"
                     /translation="MLTIKNWQLLPENDKRRCLSRPRDNSAIQENVLEIINQVQLSGD
                     KALYDLTKRFDRVNLQYLQISQEKIRQASIPQNALIAIKQAIETISSYHQSLLPENTE
                     ISTASGITIRNVYRPIQKVGLYVPGGNKTPLVSSLLMQAIPAKVAGCPIKVLCTPPDA
                     EGEINEHILVAARLCGIDTIYAIGGAQAIAAMAYGTESVIKVDKIFGPGNSYVTQAKT
                     LVAIDADGAAIDMPAGPSEVMILADTEANPEFIAADLLAQAEHGPDSQVILICDECEL
                     ANQVNQQLEIQMSYLSRIEFIKQSLANSRIIICSNQSEQLDIINSYAPEHLIINRKNP
                     EPWIEKIVAAGTVFLGSWAAETMGDYVTGSNHVLPTSGFSRNHSGLSTLDFMTRFTVQ
                     AINQEAIRNLGPAAMTLAEIEGLDAHANAVQIRLNTLGD"
     gene            complement(12213..13094)
                     /locus_tag="VAH76_001132"
     CDS             complement(12213..13094)
                     /locus_tag="VAH76_001132"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEO1385776.1"
                     /translation="MKNRLRLALQKKGRLADESLNLLQRCGLKFRIKPNALLTHVDNF
                     PIDLLFVRDDDIPTLVFDSLCDGGIVGENVLLEASLNRPDKSFRKILTLGSCTCRLSI
                     AVPEAFDYQGSGSLDGKRIATSYPNLLNNYLAERKISAEILTLSGSVEVAPRMGMADV
                     ICDLVSTGQTLEDNKLKEVDTVLNSQAVFIQTQRQLDPDMQELFAMLARRIQAVQQAQ
                     ERKYIVFHAPKAALERIAQKLPGAESPTILPLPGNNEKVAVHVVSSEGVFWNTLETIQ
                     YLGASSILVLPIEKMLE"
     gene            complement(13091..13387)
                     /locus_tag="VAH76_001133"
     CDS             complement(13091..13387)
                     /locus_tag="VAH76_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1385777.1"
                     /translation="MKIHGSFKKSALPNLMQAISLLKNEDEAMRFFTDLCTPAELESM
                     ADRWQVVPLLRQGIPYRTIHEETGVSVTTITRVARCLSFGTGGYRLIAERLEKL"
     gene            13589..13759
                     /locus_tag="VAH76_001134"
     CDS             13589..13759
                     /locus_tag="VAH76_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385778.1"
                     /translation="MKPLTRDIVFTLSFKMILLVLLWYFCVRGMHPDLSSNKEWLLGK
                     EEQTVLTPNNKR"
     gene            13764..15296
                     /locus_tag="VAH76_001135"
     CDS             13764..15296
                     /locus_tag="VAH76_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO1385779.1"
                     /translation="MIPAAEVVDLSRLQFALTALYHFLFVPLTLGLSVMLAIMETVYV
                     MTGRELWKQMVKYWGILFGINFVLGVATGLTMEFQFGTNWAYYSHYVGDVFGAPLAIE
                     GLMAFFLEATFVGLFFFGWNRLSKLQHLTSTWLLALGTNLSALWILIANGWMQHPVGA
                     YFDYQTMRMEVSSFYEMMFNPVAQAKFVHTISAGYVTGAMFVLSISAYFLLRGRNKAF
                     AKRSMTVAVSFGLASALSVVVLGDESGYVANENQKMKLAAMEAMWHTEKAPANLTIFG
                     IPDEKSFTTKYAIDIPYALGIIATRSFNTPLQGIIELIEEGKLKIKDGMIAYDALKVL
                     QKDPKNEQAKAVFTKHSENLGYALLLKKYTENVTDATEEQINQAAHDLKPKVLPMFFS
                     FRIMVACGIYFILLFATGFYLSVRHRLEKTSWYHRVAFYSLPLPWIAAELGWIVAEYG
                     RQPWVVEGILPTFMGASSLTSGQVMTSIAGFVLFYTVLAIVEVYLMVKYIRLGPDGMN
                     HH"
     gene            15312..16448
                     /gene="cydB"
                     /locus_tag="VAH76_001136"
     CDS             15312..16448
                     /gene="cydB"
                     /locus_tag="VAH76_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO1385780.1"
                     /translation="MPLDYETLRIIWWLLIGILLIGFAIMDGFDLGVAMWLPWLGKTD
                     IERRILINSIGPTWEGNQVWFILGGGAIFAAWPTLYALSFSGFYMAMLVVLLALILRP
                     VGFKYRSKLDNPVWRAFWDWALFIGGFVPALIFGVAVGNVIQGVPFYFDESLRVFYTG
                     SFFELLNPFALGCGLLSVLMLAMHGAFFIQVKTVGTLQNKAKDRARWSALLVILLFIG
                     LGAWVYYGIDGYVLKGTVPHDGPSNPSYKDVIIQAGAWFNNYKEYPVSLLVPVLCVIS
                     AFLAIIFANKSVLAFVLSGLSVGTLIATVGVSMFPFILPSSSNPKYSLIVWDSSSSQV
                     TLFIMLVATVLLLPIVLAYTAWVYRVLRGKVTESSIKAEQDSAY"
     gene            16460..16561
                     /gene="cydX"
                     /locus_tag="VAH76_001137"
     CDS             16460..16561
                     /gene="cydX"
                     /locus_tag="VAH76_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEO1385781.1"
                     /translation="MWYFSWILGTGLACVFAVLNAVWLELREEDVKQ"
     gene            complement(16674..17306)
                     /gene="pyrE"
                     /locus_tag="VAH76_001138"
     CDS             complement(16674..17306)
                     /gene="pyrE"
                     /locus_tag="VAH76_001138"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO1385782.1"
                     /translation="MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGD
                     SLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVK
                     THGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
                     TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA"
     gene            17555..17788
                     /locus_tag="VAH76_001139"
     CDS             17555..17788
                     /locus_tag="VAH76_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEO1385783.1"
                     /translation="MSQRETGHVKWFNEKKGFGFIVNQKGEDIFVHYKDIQGVGFKTL
                     HENDPVTFELDRGPKGLKAQNVVVASESVHTEE"
     gene            18299..18871
                     /locus_tag="VAH76_001140"
     CDS             18299..18871
                     /locus_tag="VAH76_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO1385784.1"
                     /translation="MLLPIQVTFRNMDPSKAVESRAIKLAKKLDCLYDKIMGCRVVIE
                     SPHRHHNKGKLYHVRIDLTVPDAELVVSRESSNKHAHEDVYVSLRDAFNAIERQLKNY
                     ANQRRGHVKRHEDSIRGGRVLEVCPIADYGYIETIDGRLIRFTGQSVVDYDINKLEVG
                     DRVRFVETVKNTQEAAASTVYVEGKRHIMN"
     gene            19031..19486
                     /locus_tag="VAH76_001141"
     CDS             19031..19486
                     /locus_tag="VAH76_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEO1385785.1"
                     /translation="MPVKKLKQFLDSHKIKYLSIAHSPAYTAQEIAASVHVSGKQLAK
                     TVIIKMDGRLAMVVLPASDHITFMKLKEAIGASDLELATESEFEGKFAECDVGAMPPF
                     GNLYGLPVLVSTKLSAQDNILFNAGSHSELMQLSFSDFEKLVKPTLVTL"
     gene            complement(19516..19935)
                     /locus_tag="VAH76_001142"
     CDS             complement(19516..19935)
                     /locus_tag="VAH76_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO1385786.1"
                     /translation="MYLSSLLKKTNRLLIKRANELIKPYAMSHAYTYILMELYIEDGL
                     TQSELVQLIEVEQPTLVRTLDRMERDGFITRKPSSMDRRAVHIFLTEKARLLQEKIDE
                     CSRILNDEILAGFSEDEKTKLQELTHRVLDNLRNPIE"
     gene            20013..20801
                     /locus_tag="VAH76_001143"
     CDS             20013..20801
                     /locus_tag="VAH76_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEO1385787.1"
                     /translation="MKSLPIYQVDAFASNLFKGNPAAVCPLDKWLSDETMQAIAAENN
                     LSETAFFVPETDGYRIRWFTPNEEVALCGHATLASSYVLFNQLGFSGEEIIFNSLSGK
                     LWVKNTDQGLVMDFPVLPYEEINFSAELQHLNLKRPFKALRSQFDLMFVYQNAEEVVE
                     AKPDLNAVAHLDYRGLILTAPGISTDVYSRCFYPGCNVPEDPVTGSAHCVIAPYWCEQ
                     LSKNHITALQGGFRQGELYCEVKGNRVLLYGNCHLYLEGRIYLP"
     gene            20798..21346
                     /locus_tag="VAH76_001144"
     CDS             20798..21346
                     /locus_tag="VAH76_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385788.1"
                     /translation="MKYLTTPRLLLRAWDEEDTIPFYRMSQDPRVMKYFPGLWSMDMV
                     KDFIIRMNKQLSQKNFTLWAAEVKDCKRFIGFIGLNAPTWNAHFTPCVEIGWRLATEF
                     WGQGYATEGAKAALEYAFLEMHIPEIVSFTVPDNSRSRSVMDRLGMIRDIAGDFLHPR
                     LASDHRLAKHVLYRIQNPLIKV"
     gene            complement(21470..22129)
                     /locus_tag="VAH76_001145"
     CDS             complement(21470..22129)
                     /locus_tag="VAH76_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEO1385789.1"
                     /translation="MEQWHQFTLLFPLIILVFISLISPGPDFAITVKNSLAYSRRSGL
                     LTALGIALGSLVHISYTLLGLGLFITKTPIILHIIQYLGAGYLFYIGLKNLGAKPNAD
                     QIGSYASYNDLKPSEAFLNGFITNLLNPKAMLFFISLFSVLMPREISIPNKLFLAIIL
                     FLQTLLWFSFVAFSLSGKNTRTKFQRVSYWIERITGVILIALALKLLLSQLGSVDISL
                     A"
BASE COUNT         6703 a         4055 c         4541 g         7013 t
ORIGIN      
        1 atgtattact agtttacaaa ctttttcgtc gagacaaaca atttcatagc gctggctgta
       61 atgaacataa ggaatattat caacaatata atttacaatt aagacatttt ccggtactga
      121 gcggtttagt ttaagaaaat ggagaaagct accaccactt ttgtcataaa catcagtaat
      181 aaaaatcgcc gaaacaccgg gcagttgatt taaactttta tattgtagtt gttttaaaat
      241 tttagagata tcttctttgt tcatataaaa attatctctt aaatactcta agccatagcg
      301 ccaactgtac ataatgaagg cgataacgaa agctaaaaca attggaaccc agccacctgt
      361 aatgaattta tgagtattag ctcctaaaaa ggctaaatca atcaaaagaa atagaccaaa
      421 gagaaatatc acattgaatg ttgaccatct ccatattgaa acagcggcgt aagcaaccat
      481 ggcatcaact aaaagcattt caaggtttac agcaattcca taagcatgag ccaggttatc
      541 cgaggttttg aaagccaggc aaaatgccat ggtaccgata aatagaataa aattgatttg
      601 cggaacatag atttgccctg aatgcagcat ggaggtctgg acgattggaa ttttggggca
      661 taaacctaac aacaccgctt gtttggttag tgaaaaagtt gctgaaatta ctgcttgaga
      721 tgcgatcact gtagcaacag ttgcaataat aattaaaggc agataaaacc atggtggcgc
      781 aatcatgaaa aatgggttgc ttatttcttc ggggcgtact atcaggttag cgccttggcc
      841 aaaataattt aatagcaggc agggaagcgc tataaaaaac cagctgaatc ttattgggtt
      901 tttaccaaaa tgacctatgt ctgcaaataa agcttctcct cctgttacta caaggaaaat
      961 acctcccagc aaaaagtatc ctcgcaagcc ttcgtcaacc aaaaacgcaa tggcgtagta
     1021 ggggttgatt gcttgcaaaa caactggatg ctctacaatt tgcaatattc ctaatatggc
     1081 tatggttata aaccaaataa gtataagtgg gccaaaaagg tagcctatcc ttgctgtgcc
     1141 tttggcttgc aaagagaaaa gcaaaacgag gatcacagaa gcaatgggga gaacgtaagg
     1201 gtagagctta tcagaaagag ttccaagacc ttctaccgca ctgacaacgg aaatggccgg
     1261 ggtaagcata ccatcaccta ataacaaacc ggcgccaaat atggcgacta tgtaaaatag
     1321 aggttgatat ttggtgctct tatgcttcat taaagcaaga agtgccaaaa ttcctccttc
     1381 cccatcatta tctgcccgaa aaataatcat tagatactta aaagaaacaa cgataatcaa
     1441 tgaccaaaaa attagcgata atacgcctaa tatattgaat tgattgatag gaatgcctga
     1501 taaggtaact tttaaagcat acagtgggct agtgcctata tcaccaaata caacacctag
     1561 agccccaagg gcaaggccat aagtgaacct gttttttttc atttcatagg aggaataacg
     1621 catgtgctat caccaaaagg cattaattgc cagtacatag gtcatataat gggaagtatt
     1681 tactccactc acgggaaacc aaaccccacc aattacccca atgttttcat tgaagttgta
     1741 ttctattgcc ggagctagtg cttcttctct aaatgagccg ttgccaataa ctacgggggc
     1801 agcatccaaa tcactgacat tgaccggcgg gcttccaatg ctgttaaagt ttaaaatacc
     1861 gttaaatcga gtagctttac cagttgcaat atagccttcc attacggcaa cccagtgttg
     1921 agtaagagta aattcgaagg ccaagtctaa gttattttca atgccgggtt taactgtccc
     1981 atgagtattt attgaaccac cataactgct tagtccatca acatgaacat tactggaaaa
     2041 aagacgtgat gcaataaatc gtgttctgag ataatgggtt ttaaaaactt gtaataaata
     2101 ttgaaagtct aagcccacta acgtttggta gcttccaaga ccagtggcat cagtaccaag
     2161 taaagccgga ttcaattgat cataacgtcc agtgggtatt gtttcctgta aaaggacacg
     2221 aaaatcgatt cttttagggg aacctttttg ctccaagact tgtattccca atgcagttga
     2281 aacatcggcc aaatggttct catggactcc gcgtgtcgag ttgaataaat aaggcacggt
     2341 caactgcaca tccagccagt cagtaaagcc atgagtgagt attgggttgg cggcaaaggt
     2401 tttaaacttg ggtgtggata tccgttgtcc agatgcgttg tattgcccat cagtaaagac
     2461 atcaatacca taaacttcga ggttggtatg acccttggcg acagtatggc ctgcgggtgc
     2521 caataaaggt ccggtgaacc agggaccggc ataagcaacg ctcactgaag atgaaaatag
     2581 aaaaatggct aggtattttg taaattgccg catatttgtc cttctttcca ttgcaagtat
     2641 aattcttgat agtgtagatt aactgacttt taggttttgg gaaatagaga atgaaagcga
     2701 aagttatttt agtgaatgga aagcaaaaca ctgtattaag aggtcatccc tggatttttc
     2761 ccaaagccat agctcaatat tctggcaagc tgattacagg tgaattggtt gagataattg
     2821 gctctgaagg tgagagttta ggattcggag cctataatga gcactcttta tatagagtaa
     2881 gggttcttgc tctctccaat gaacagataa atagtttcga ttacaagtct ttaattactc
     2941 atagaatcaa gcaagccagt ctggtgagac gatgtcttga tttaccaaat gaagaaacta
     3001 atgcttatcg attgttcaac agcgaagcgg atggactctc tggtttaacg attgattgct
     3061 ttaatgacta ttgcgttata gcaagttctg cttattgggt agaattgaat aaatcgatta
     3121 ttgttcaggt attgcaagag ttattcccgc ataatcatat catttggctt cctcaaaaca
     3181 agccgttatc tcaagatggc tggaaagaaa ttcatacaga gaaaagtcag ggtagcactc
     3241 aggttttgga agaaggtgtt ctgtatcatg ttgaatttgc gcaaacccaa aaaactggat
     3301 tgtttcttga ccaaagagaa aatcacaaaa ggatagcaaa actatccagg ggaaaaagag
     3361 tcttggatct ttatactttt acgggagggt ttgctttgca cgcagcgaaa gcgggcgctc
     3421 ttcatgttac ggctgtagat agctcggcgc aggcaataga gcaggctaaa aataatgctg
     3481 ttttaaatca cttaaatacc attgaattta ttaaggcaga tgccagagaa tatctgaaaa
     3541 tggctggcga atatgatgtg gtcattcttg acccgccaaa actggttcct tcacaaaaac
     3601 atttacagca agctaaaaat tattaccgat ttttacatag agaagcattt aaatatatga
     3661 aaccaggatc tttattgatg acttgcaact gttcttcagc gctttcatcc caggattttt
     3721 gttccctggt aagcgctcag gctgttgcga tgggaaagca ggcgcgtatt ttaggcgtat
     3781 acgggccagc aagctgccat cctaccttgg cagcctttcc agagggaaat tatttaacag
     3841 ctgttttatt ggcattggta taaaaggaac cttgattttt aaatcaagga tggtgttagg
     3901 ttgatttttt tgtataacat ttgattagtt tggctttaat atgtataatt ggcgcaaatc
     3961 gcaatagaga aaattaatca ttttctaatg agtttagcat tgaaaatcaa gtcaattatt
     4021 ttatcttttt ttcttgtttt gatggctaca gtagcttgcg ctacacaagt gagtttgaga
     4081 gataaaattg ggcaaatgtt aatattagga ttccagggca aagtggttga cgagaaatca
     4141 cctattgccc aggcaattga gaaaaacaac attggtggtg ttattctttt tgattttaat
     4201 cagcagtcac aaacttttga taaaaatata gaaagtccgg aacaagtcaa ccaattaaat
     4261 aatcagttac aaacacttac gcaaaaagcc aaccgtaaat atcatagaga taatttacca
     4321 ttacttattt ctgttgatta cgagggggga aacgttaatc gattgcatcc ccgttatgga
     4381 ttccctgaaa tgccttcagc aaaagacatt ggctccatgt ctctggaaag agctgatgca
     4441 tcagcagaat tgatggcaaa tactttaaaa tcaacaggat ttaatttgaa ttttttccct
     4501 gaattagatg tgaatataaa tccagataat cctatcatag gcaaaaaaga caggagtttc
     4561 tcttcgatac cggaaattgt cactcaatac gcgcaacttt atacagagca atttataaaa
     4621 catcaaatac attgttctta taaacatttt cctggacatg gcagttcatt atctgattca
     4681 catttgggtt ttgtagatat aagtgacacc tggagcgagc aagaattaat ccctttttta
     4741 caattattat ctcaacccaa tcattgcgat atggtgatga tagctcatat tgtgaataga
     4801 aaactggata tgacagggct acctgccagc ctttcttacc agatcattaa cggattattg
     4861 cgtcatgatt taaaatttaa cggggttgtt atcaccgatg atatgcaaat gaaagcaata
     4921 acgaattatt atggattaga aactgctgtt actctttcaa ttaatgcagg agcagatatg
     4981 ctaatatttg gtaatcaatt ggtagaaaaa tttcaagact cgacagaaat cattgacatg
     5041 atcgagcaaa aagttcgatc gggagagatt tctgaacaaa ggattaatga agcttatcaa
     5101 cgaattgtaa aaatgaagag gtcttttaat tattagaagt ttttctcaat ttactctgac
     5161 aaaagtactt tgataatctg caagctatag ataagctgag gtatatatgc ggtgcaggtc
     5221 aattatcaaa aaaactaata taaacaatgg gatttttcag attagggtgg cattgcatga
     5281 aaaacgtgct gaaaagagaa gatgcggcga ataaaaaatt gggagttttg atttatgaaa
     5341 acgtccagcc tatggatgtt attgggcctt gggaagtatt tgcaacctgg aaaactattc
     5401 ttgatgcccc tattgatatg tttctggtag cagaaaccgg caatgaagtt tgttgcgcaa
     5461 atcaaattag cctgaaacct cattgtgaat ttaataattc tccacaattt gattatttac
     5521 tggttccagg tggggttggt agaagagaac aagtaaataa tccacgtttg atttcttttt
     5581 tgaaaaagca ggctcaaaac actgactatt tgctttccgt ttgtactgga gcatttttat
     5641 tgcatgcggc agggctattg attcatcatg aagcaacaac ttattggcgc gctatgcctg
     5701 aattaaagtc ttttgaggat gtccaattgg ttgaaaagcg tatcgttaag tcaggtaaag
     5761 tttggactac tggtggaatt tccagtggca ttgatctggc atttgaattt atttcaacca
     5821 ttgctggcaa tgatgttgcg ggaaaagtac agttgctttt tgaatacttc cctgagtgca
     5881 agctgtattc ccaacctgct atggtgagtg agttgccttc ttactatgca actaaaaagg
     5941 atgaggcagt gttggaaatg aatttgcctg attacattaa agagtatttc aagaaaagtt
     6001 aatttcttaa tagtagttta aaaaattaac tagtcttgat tgataattcc tgaggattct
     6061 ggttatagag atcttctatg gaataagcaa ttaagtatgc acacttctgt ctcttgcctg
     6121 taaacacaat aaaacatcat aaaaacttaa ctcacaagct ttgagaagca ctaataaatg
     6181 ataaattaaa tcagatgctt cgttgataag ttcttcttta ttgttgctca cagcagcgat
     6241 cactgcttct accgactctt ctcctaattt ttgagcacat cggttcacac cagaatccaa
     6301 gagttgagca gtataactgt ttggattttt ttcctcagca cgttcattaa ttaattcaat
     6361 caaaccatca agaaaaccaa gagtataatt attatgtggc tgatagcagg ttgaataacc
     6421 taaatgacaa gcgggtcctt taggaataac ctggatcaaa agactgtcat tgtcgcaatc
     6481 ggcactaata tgttgcagag ccatactatt gccagaagac tctcccttac gccacagtct
     6541 tttccgactt ctgctgtata aggtcagttg tcctgtagtt agtgtggcaa ttaatgcttc
     6601 ctgattcata taacctagca tcaatacttt accattttct gcgttttgga ttattgccgg
     6661 caatagtcca ttcatttttt gccaatcaag agagttaatg tccaagtcac tcatagtctt
     6721 acctcaatat tttctgtttg taatgctagt tttatgtcat tgattgccag tattttgtca
     6781 tgaaaaacgc ttgctgccaa agccccgctg acatgagact ttttaaatac ttcaacaaaa
     6841 tcattaattt ttccagcacc gcccgatgcg atgagaggaa cttggcagag gttaatcatt
     6901 tctgatagct gtgcaatatc atatcccatt cgaacgccat cagattgcat gcaattaagg
     6961 actatctcgc cagcaccgcg gtcttgaatt tcatatatcc attctcgagt tttgcgtttt
     7021 gaattggtac ttttattttc attacctgta tactgataga cataataatc atcaattatc
     7081 cactgactat cgacccctat taccacacat tgttttccaa attctttgca taattcgtta
     7141 atcaatgaag ggttttccaa cgcaggactg ttgattgaga ttttatctgc tccggcattt
     7201 aatattgatc ttgcttgatg caatgttcgg atacctccag ccactgtaaa tggaatatta
     7261 atagtagccg ccactttatt gacccaaaca ggacaaacag aacggtcatc gctacttgct
     7321 gtaatatcat aaaataccaa ttcgtctgcg cctgatgctg agtagtgagc agctaatggg
     7381 agtatgtctc caattattct atggttgcga aattttactc ccttgacaac ctgattatcc
     7441 agaacgtcaa gacagggaat gattcgcttg gttaacataa taactcctcg aaattcacat
     7501 tatcttgata aagcattagc ccaaggatta cggcggatat tcctaatgag ttcagtgttg
     7561 taatatcctg catatggcgt actccccctg aggcttgcca ggcaatctga ggaaaatatt
     7621 caactgcttg ttggtacaaa tcgaaattag gcccattcat catgccatcg catgcgatgt
     7681 cagtacaaag tatgttcttt atcccatatt tttcgtaata ggacactact tcccaaaggc
     7741 tgcttgtact attattttgc cagccattaa tcgcaaggag ggggattttt gtatcaacac
     7801 gaacgtccag agcaagaaca atattttcag gtttaatctt ttcaatgatt tgtattgtca
     7861 aatcagaatt tgtaatagcc aggctgccaa tgaccaattg tgaaattcct gcgttgctgc
     7921 attccaaagc ctgttctata ctcctgattc cacctccggc ttgaattggt attcctgtct
     7981 tttgcatgga acaaatgagc tccaactgtt gcatttttcc gctgcgagca ccatccaaat
     8041 cgactacgtg aaggcgtttg gcgcctagtt tggcaaaatg caaagccctt tctatgggaa
     8101 aagcgctaaa ttgcgttact tgatcaaacc gtccttgttt taagcgcaca catcgtccag
     8161 attgcaaatc aatagctgga ataactaaca tgtagactcc aaagataaaa agttatttag
     8221 taataccatg ccaacatcag ctgatttttc tggatgaaat tgcataccat aaaaattgcc
     8281 tttttttaca actgcagtga actcttcatg atactgacat cgagccaatg cataatcatc
     8341 tgcttttaga gcatagctat gaacaaaata aacataatca ctattattta atccttgttg
     8401 taatgatgat gttttttgcc aatgaagatt gttccatccc atatgaggaa caggatgatt
     8461 tctttcagct tttaataact ctgcaaatcc aggaatcaaa cccaggcagg gtatgtcgtc
     8521 ttcttcgctg tattcaagca ataattgcat tccaagacag atgccaagaa gtggttgggt
     8581 taaggttctt aaaacgtcta ttaacccatt ttgctgcaaa gctgtcatac cagaacgtgc
     8641 tgttccaacc cctggcaaaa tgacatggct tgctttttgt atttcttcag catcgtgggt
     8701 cagtgcaaaa tgccctccca atcgaatcaa tgcatttgtc agagaagtta aattattccc
     8761 actgacatca atcacagcaa tcataataca cccttggttg aaggtagata attattcgtt
     8821 cttgagcatg cctgtcgcaa ggctctgcct aaaactttga agcatgcttc gatcatgtgg
     8881 tgatgattgg tgcctgttac ctcgatatgg atagttgccc ccaacgcgct cgatagcgat
     8941 tggaaaaagt gaggcaccat ttctgttgcc ataccgccaa caaattcacg agtaaactga
     9001 cctttgaagt cacaaaagct tctgccgcta atatcgattg caacgcaagc taatgattca
     9061 tccataggaa gggtataacc gtaacgatta attccccatt tgtcacccag cgctgttcta
     9121 attgcttctc ctaacgcaat ggctgtatcc tcaatcaagt ggtgctcatc tacttccaaa
     9181 tctccagaag cttgtaatcg caaatcaaag cctccgtgct ttgctacttg ttccaacatg
     9241 tgagtgaaga aaggtatagg agtgtttata acactggttt gatcgctatc cagttgaact
     9301 gacaaatcta ttgctgtctc ttttgttttt ctttgcacag atgctttgcg agcatcgttg
     9361 ataattgcag aagccaccat ttcccaagta tgtgttttcg aaatgggcaa gaaattaacg
     9421 cccagattat cagcaaactc tttatcagta tcccggtcgc caattaccca tgaatattgt
     9481 ttggcaaatg ctgtttcttt taagtaaggt tcgagcaagc cagttttagg ttttctacaa
     9541 atacaattat cttcaggggt gtgagggcat ataaaaatct catcaaaaag aatgccttga
     9601 gaggaaaaca gatccaggat aaattcatgg caaacagcaa aatcctcttc tggaaaagct
     9661 acggttccta tgccattttg attggatacc ataatgaagg taaagccagc tttttgtaat
     9721 tgcaataggg ctggtattac tccagaagtt aatttaattt tatccagaga gtctacttga
     9781 aaatcaaagg gttcttcaac taaagtgcca tcgcggtcaa taaataaaat ctttttcata
     9841 atttaatcct gctacatcag cgttaaatga ggataaggca ttaatcaata attgattttg
     9901 ctcatcatga cccaccgtaa tacgaaggtg atcatgcaat aaagaggacg aaggaaaatc
     9961 tcttactgcg atgccataat gggcaagcca agtggttaat tgttttgaaa atcgtgtttg
    10021 aatgagaatg aaattggttt cagtaggata aattttttga atgattggat tatcggtgaa
    10081 tttttttata acccaggcac gagatgattt gatttgctca atagcttttg tgaaccaatc
    10141 gctattattc aaagctcttt tagccagctc catactagga gtggcaatgg aatagggggc
    10201 aatgatttta ttaaaagctt gaataatagg actctgggct ataatgcagc ctaagcgtaa
    10261 tccagccagc ccaaaggctt tcgacagggt tcttaagaca atcagatttt caaactctcc
    10321 aattaaggac gtagcgcttg gtgcatttgc aaactctata tacgcctcgt ccacaactat
    10381 aatagattga ttcgcataca gttcacatgt cttggcaatt aaatttaaat ccaccaaatt
    10441 accagtcggg ttattgggac tacaaaacat gataactttg cagtttggtt tccagctatt
    10501 ttcaatttga tccagggtga ggcgaaaatt agtccttcta tccaaaggac attcgatcaa
    10561 ttctgcttgt tgtagccgaa cataaaaagc gtacatagga aaagtagggg gaaattgcat
    10621 gaaagcgtct tttccggcag ttagaaaaag tctggttgtc aaatcaatgc catcatcact
    10681 tcctctggtc agtatgattt gatcggaatt gatttgataa cgttttgcca attgcttttg
    10741 cagctctatt tgtaagccaa tgtttgggta ataatttaaa ttgtgctccc ccatagtaac
    10801 tggtgaccaa ggcagttcat tggcatgtga tcgatagcga gcactctcac caccggggac
    10861 ataattttga ctatttaata gatctgcacg aacaagattg agtatggaca tttaatcccc
    10921 cagagtattg agtcttattt gaacggcatt ggcatgagca tccaaaccct ctatttccgc
    10981 taaagtcatt gccgctggac ctaaattacg gatagcctcc tgatttatgg cttgcacggt
    11041 aaatcgtgtc ataaaatcaa gagtagaaag cccgctatga tttctggaaa aaccactggt
    11101 aggtaagacg tgattgcttc ctgttacata gtcacccatt gtttctgcgg cccaagagcc
    11161 aagaaatact gttccggcag ctacgatttt ctcaatccat ggttcggggt tttttctgtt
    11221 aatgatcagg tgttccggag cgtaggagtt gataatatcc agctgttccg attgattaga
    11281 acagataatt atcctgctat tcgcaagaga ttgtttgata aattctattc ttgacaagta
    11341 actcatctga atttccagct gttgattaac ctggtttgcc aattcacatt catcacaaat
    11401 taaaataacc tgtgaatccg ggccatgttc agcctgggct aataaatctg cagcaataaa
    11461 ttcagggttt gcctcagtat ctgcaagaat cattacttca gatggcccag cgggcatgtc
    11521 aatcgcagct ccgtcggcgt caattgcaac taaagttttt gcctgagtga catagctatt
    11581 acccggtccg aatattttat ccactttgat gacactttca gtgccataag ccatagctgc
    11641 tatggcttga gctccaccta tagcgtaaat tgtgtcgata ccacataatc ttgcagctac
    11701 caggatatgc tcgttaattt ccccttctgc atcagggggg gtacaaagca cttttattgg
    11761 gcagcccgct acctttgccg gtattgcctg catgagaagt gaagaaacta aaggtgtttt
    11821 atttcctcca ggcacataca agccaacttt ttggattggt ctgtagacat tgcggattgt
    11881 aattcctgat gcagtcgaaa tctcggtatt ttcaggcaac aaagactggt gatatgacga
    11941 gattgtttca atagcttgtt ttatggcgat taaggcattt tgcggtatgc tggcttgtct
    12001 aattttttcc tgtgagatct gtaaatattg taaattgact ctatcaaacc gtttggttaa
    12061 atcgtaaagt gctttatctc ctgataattg gacctgattt ataatttcca gcacattttc
    12121 ctgtattgca gaattgtctc gtggccttga aaggcatcgc cttttatcgt tctcaggcag
    12181 taattgccag ttttttatcg ttagcatatg aattactcca gcatcttttc aataggtaat
    12241 accaggatgg agctggcacc taaatattgg atagtctcta aagtattcca aaaaacacct
    12301 tcactggaga ctacgtgcac ggcaaccttt tcattatttc cgggtaatgg gagtatggtt
    12361 ggtgattcag ctcctggtaa tttttgagca attcgctcaa gggcggcctt gggcgcgtga
    12421 aaaacaatgt attttctttc ctgagcttgc tggacagcct gtattcttct ggcaagcatg
    12481 gcaaacaact cttgcatatc aggatccaat tgtctctgag tttgaataaa gacggcctga
    12541 ctgttgagaa cagtatcaac ttctttaagt ttattatctt cgagtgtttg gcctgtagat
    12601 accaaatcgc atatgacgtc agccattccc attcgtggcg ctacctcaac cgagccggat
    12661 aaggtcagaa tttccgcact aatttttcgt tcagcaagat aattgttgag taaatttgga
    12721 tagctcgtgg caattcgctt cccatccaga ctcccggagc cttgatagtc gaatgcctct
    12781 ggtactgcta tggataaacg gcaagtacaa ctgcctaatg ttaatatttt tctaaatgat
    12841 ttatctggcc tgttaagaga ggcctccaac aggacatttt ctcctacgat gccaccatca
    12901 cataaactgt caaatacaag agtagggatg tcatcatcac gaacgaagag taaatcgatt
    12961 ggaaaattat caacatgggt taataatgcg tttggcttaa tgcgaaattt taaaccacaa
    13021 cgttgtagga gatttaaaga ctcatctgct aatcgacctt ttttttgcaa ggccagacgt
    13081 aaacgatttt tcacaacttc tccaatcttt cagctattaa acgataacca cctgtaccaa
    13141 agcttaaaca acgagcaact cgcgtgatgg ttgttacact gacccctgtt tcttcatgta
    13201 tcgttctgta agggattccc tgacgtagca aaggaactac ttgccatcga tcagccatag
    13261 attctagttc ggcaggagta cataagtctg taaaaaaccg catggcttcg tcttcgtttt
    13321 taagcaagct gatggcttgc atcaagttag gcagcgcaga ttttttaaaa gatccatgta
    13381 ttttcatatt aatgtattag cgtgttgtaa catattaatg atgataatga ttctatttat
    13441 cattgtcaat aagttagtga aaaaaaaatc tggatagggt agagtataca ggtctctttc
    13501 ccggaaactg gattggctga aaatcaaaag gcagacgtaa atatttttta acagagactc
    13561 tcgatatgat aaatcctgag gaaatgtcat gaaaccacta accagagata ttgtatttac
    13621 cctttcattt aaaatgatat tgcttgtttt gttatggtat ttctgtgtca gaggaatgca
    13681 tcctgatctg tctagtaata aagaatggtt gttaggaaaa gaagaacaaa cagtacttac
    13741 cccaaataat aagaggtagt tttatgattc cagctgctga agttgttgat ttatcaagat
    13801 tacaatttgc attaactgca ctatatcatt ttctatttgt gccccttacg ttggggttat
    13861 cggttatgtt agcgatcatg gagacagttt atgtgatgac tggccgagaa ctctggaagc
    13921 aaatggtcaa atactggggt attttgtttg ggattaattt tgttttggga gttgcaaccg
    13981 gtctgactat ggaattccag tttggtacta actgggctta ctattcacat tatgttggag
    14041 atgtatttgg tgctccatta gcaattgaag gactaatggc cttttttctg gaagctactt
    14101 ttgtggggtt atttttcttt ggctggaaca gattgtcaaa attgcagcat ctgacctcta
    14161 catggttatt ggctttaggt actaatcttt cagcattatg gattttaatc gctaatggtt
    14221 ggatgcaaca tcctgtaggt gcctattttg attaccaaac gatgagaatg gaagtcagca
    14281 gtttttacga aatgatgttt aaccctgtcg ctcaggctaa gtttgtgcat actatcagtg
    14341 cgggatatgt gacaggagcc atgtttgtct tgtccataag cgcctatttt cttttgcggg
    14401 gacgaaataa agcgtttgcc aaacgctcaa tgacagtcgc agtttctttt ggtttggcat
    14461 cagcattgtc tgttgtggta ttgggagatg agagcggtta cgttgccaat gaaaaccaaa
    14521 aaatgaaatt ggctgcgatg gaagcgatgt ggcataccga aaaagctccc gccaatttga
    14581 ctattttcgg gataccagat gagaaaagtt tcacgacaaa atatgcgata gatattcctt
    14641 atgctcttgg tattattgct actcgttcgt tcaatactcc tttacagggc attattgaat
    14701 taattgagga aggaaaatta aaaatcaaag atgggatgat cgcatatgat gcattgaaag
    14761 ttcttcagaa agatccaaag aacgaacaag ccaaggctgt ctttacaaag cacagtgaaa
    14821 atctgggtta tgctttgtta ctcaagaagt atactgaaaa tgtaacggat gccactgaag
    14881 agcaaataaa tcaagcagcg cacgatctca aacctaaagt actgccaatg ttcttctcat
    14941 ttagaattat ggttgcttgc gggatatatt ttatacttct tttcgcaacc ggtttttatt
    15001 tgtctgtaag acatcggttg gaaaaaacat cttggtacca cagggttgca ttttactctt
    15061 tgcctttacc ttggatagct gctgaattag ggtggatagt agcagaatat ggtcgccaac
    15121 cctgggttgt tgaaggtatt ctgcctactt ttatgggagc ctcctcttta acatcagggc
    15181 aagtaatgac atcaattgca ggctttgtat tgttttatac tgttcttgca attgttgagg
    15241 tttatttaat ggtcaaatac atacgtcttg gcccagatgg gatgaatcat cattgatgaa
    15301 aggagaatta aatgcctttg gattatgaaa cactcagaat aatttggtgg ttattaatag
    15361 gtattttgct tattggtttt gccattatgg atggatttga cctcggtgtg gcaatgtggc
    15421 ttccctggct aggcaaaaca gatattgaga gacggatatt aattaacagc attggcccaa
    15481 catgggaggg taaccaggtt tggtttatac tgggcggagg cgctattttt gcggcttggc
    15541 ctactctcta tgctttatct ttctcaggtt tttacatggc gatgctggtt gttcttttag
    15601 cgttgatttt aagacccgtt ggctttaaat acagatcaaa gctggataac cccgtatgga
    15661 gagcattttg ggattgggct ttattcatcg gtggatttgt tccggcttta atctttggag
    15721 tagcggttgg aaatgtcata caaggtgttc cattttattt tgatgagagt ttaagagtat
    15781 tctatacggg aagttttttt gaattactca atccgtttgc tttagggtgt ggattattat
    15841 ctgtgcttat gcttgctatg catggtgctt tttttattca agtaaaaact gtagggactt
    15901 tgcaaaataa agcaaaagac agggctcgct ggagtgctct tttggttatt cttttattta
    15961 ttggattagg ggcttgggtt tattatggta ttgatggtta tgttttaaaa ggaacagtac
    16021 cccatgatgg gccatcaaat ccttcttaca aggacgtcat tattcaagcc ggagcatggt
    16081 ttaataatta taaagagtat ccagtatcac ttttagtgcc tgttttatgc gtaatatccg
    16141 cttttttagc gattattttt gcgaataaaa gtgtgttggc ttttgtgctg agcggattga
    16201 gcgttggtac tttgattgca acggtagggg ttagtatgtt tcctttcata ttaccctctt
    16261 cttcaaatcc taaatacagt ttgatcgttt gggatagctc ttccagccag gttactttgt
    16321 ttattatgtt agtggcaacg gtattattat tgcctattgt gcttgcctac acggcttggg
    16381 tttatcgagt gttacgaggt aaggtaactg aaagttctat taaagcagag caagattctg
    16441 catattagga ggtttttcta tgtggtattt ctcctggatt ttgggcacag gactggcttg
    16501 tgtgttcgcg gtgcttaacg cagtatggtt ggaacttaga gaagaagacg tcaagcaata
    16561 gacagaggca atatcagtgt aagttgctga gcttcatgaa tagctcagca actgaaaaca
    16621 caatgagtga tgattgtctt actctttttt aaggaaaatc tgtaatccta atattatgcg
    16681 ccataacgct cttgatagga ctccagtttt tgtacctgtt cgtattgatt gtcatttttc
    16741 agaaattcta ttagatcaaa caaattgata attgaataga cttcaattcc ttgctccctg
    16801 atttctgaca gggttgattt ctcagttaat cctcgttcgc atctgtccag agcgataatg
    16861 acgccacgaa gaattcctcc attctcttta atcagggttt gtgattctct gaatgctgtg
    16921 cccgctgtaa tcacatcgtc aatgatcact gtccttcctg ttagcggcga accaattaat
    16981 tgtccgcctt ctccatgagt ttttacttct ttacgattaa aagtcactgt gatgtccctg
    17041 cctaactcag ccaaggccac tgcagttgcg gtagcgagtg ggataccttt gtacgctggt
    17101 ccaaacaagt gctcaaaaga aacttcttgt tctaaaaggg tttttgcata aaattgacct
    17161 aacttacgta aggaatcacc gtgataaaat aaaccagcgt taaaaaaata gggacttatt
    17221 cgtcctgatt ttaaagtaaa ttcaccaaat ttaagtactt gacactccag agctaatttt
    17281 ataaaagatg atttggtatg attcattctg gtttcctaat acaaaatttt attgaaaaca
    17341 atcacatatt ttaaaaaaat ccttaacaat caacaaaaaa ctgatatgct aatcatagtg
    17401 caggatttat ccttgcatgg aaaagggttt attatagcgt aaattgctaa ttagggagtg
    17461 atttattttt tcactttaag ttgcgctaaa gtgaactttt atttatttaa ataacaattt
    17521 tgatgtatct gtgtaagtca aaggggaaat gcgaatgtcg caaagagaaa caggccatgt
    17581 taaatggttt aatgaaaaaa aaggatttgg ttttattgtt aatcaaaaag gcgaagatat
    17641 tttcgtgcat tacaaagaca ttcagggagt tggttttaaa acgctgcatg aaaatgatcc
    17701 agttaccttt gaacttgatc gaggacctaa gggtttgaaa gcacaaaatg tggtagtggc
    17761 tagcgaatca gtacacacag aagaataagc cagatgattt tatctggtga ggctacgctt
    17821 tattttttaa agcctcgtta aagtacttaa gagcatgttt atagaaacct gagagtttga
    17881 cataaaatcc ggtaatttta tagtctactg ccgagtcaaa aagtacagct gttccaaatc
    17941 taaaatgaat ggagaaggtg gctgacttga ggacgagtgt aagttaaaga gaatggtctg
    18001 aggcgatcat gcttcggagt gcagcgtcga actcttaggc ttatccacgt atgcaatccc
    18061 ccccaaaaaa gtatcagtgt cctgttgtta tatgctcatt aagtttcaga tatgaagtat
    18121 ttgtatgtca actcaattca ttttttatac cattttttta gtctggtttt aataatttta
    18181 tttttattgt taattcaagt gcttatcatt tttctattga aagcaacatt ttttttgtct
    18241 atagattagt aaatgagatc tgaaattatt cataatcaaa acataaggga ggttaaaaat
    18301 gttacttcct attcaggtga cattccgaaa tatggatcca tctaaagctg ttgaaagcag
    18361 ggctataaaa cttgctaaaa agcttgattg tctttatgac aaaataatgg gttgtcgagt
    18421 agtcattgag tctcctcatc gccatcataa caaaggaaag ctttatcatg tacgtattga
    18481 tctgacagtt cctgatgctg agttggttgt gagccgtgaa tcatctaaca agcatgcgca
    18541 tgaagatgta tatgtttctc taagagacgc ttttaatgcg attgagcgcc agcttaaaaa
    18601 ttatgcaaat caacggcgag gccatgtcaa gcgtcatgag gattctattc gtggcgggag
    18661 agttcttgaa gtatgcccca ttgctgatta tggctacata gaaacaattg atgggcgtct
    18721 gatacgattt acaggccaaa gtgttgttga ttatgacatc aataaattag aggttggtga
    18781 ccgagtccga tttgttgaaa cggttaaaaa cacacaggag gctgctgcaa gtacggttta
    18841 cgttgaaggt aaacgccata ttatgaatta acaggggtat cagaggccta ttggtaattt
    18901 gcagtttgct gttgaataat tgtttaggcg aaatgactgc ttccaaacaa atgattcaaa
    18961 attttaaata agggcacttg agagagggta ttttctgaaa gaatgaatta gtatcgaaaa
    19021 ggagtttgtc atgccagtta aaaagcttaa acaatttttg gacagccata agataaaata
    19081 tttgtctata gctcattcac cggcgtacac tgcacaagaa atcgctgcct ctgttcatgt
    19141 ttcaggaaaa caactggcaa agacagttat tataaaaatg gatggacgtt tagccatggt
    19201 agtattgcct gcaagtgacc atattacatt tatgaaactt aaagaagcta ttggtgcttc
    19261 tgatcttgaa cttgctactg aatcagagtt tgaaggaaaa tttgcggaat gtgatgttgg
    19321 agctatgccc ccattcggta atttgtatgg tttaccagta ttggtgtcga ctaaactgag
    19381 tgcacaggat aatatcttat tcaatgcggg ttctcattcg gaattgatgc agctctcttt
    19441 tagtgatttt gaaaaattag taaagccaac attggtgacg ctatagaatg aagcgatgag
    19501 aaatatcatt ataaattatt caatgggatt ccttaaatta tcaagaacgc gatgagttag
    19561 ctcttgtaat tttgtttttt catcttctga aaaaccagcc agaatctcgt cgttaagtat
    19621 tctggagcat tcatctattt tttcttgcaa aagtcgggct ttttctgtaa gaaaaatatg
    19681 cacggctctt ctatccattg agcttggttt tcgagtaata aacccatctc tttccattcg
    19741 atccaaagtt cttacaaggg taggttgctc tacttcaatc aattgaacta attcagattg
    19801 tgtcaaacca tcttcaatat acagttccat taatatataa gtgtaggcat gactcatagc
    19861 atagggttta attaattcat tggctcgctt gattaacaaa cgatttgttt tttttagcag
    19921 agaggaaaga tacatcaatt tctcattatt aaatagcttg ctatataata acaggctatg
    19981 ttattattcg caagttgttt taggagacat ttatgaaaag cttaccaatc taccaagtgg
    20041 atgcatttgc ctcaaattta ttcaaaggta atcctgccgc tgtttgtcct ttggataaat
    20101 ggttaagtga tgagactatg caagccattg cagctgaaaa caatttatct gaaacagcct
    20161 tttttgttcc cgaaaccgat ggatacagga tccggtggtt tacccccaat gaagaagtgg
    20221 cattgtgcgg ccatgccact cttgccagct cttatgtact ttttaatcaa ttgggctttt
    20281 caggggaaga aattatattt aatagtttga gtggtaagct atgggttaaa aacacagatc
    20341 aaggcttggt tatggatttc ccagttctgc cctatgaaga aattaatttc agtgccgaac
    20401 tgcagcattt gaatttaaaa aggccattta aagcattgcg aagccaattt gatttgatgt
    20461 ttgtgtatca aaatgcagaa gaagtagttg aggcaaagcc tgatttgaat gctgtggctc
    20521 atcttgacta tagggggtta atattaactg ctccaggaat atcaactgat gtttattctc
    20581 gttgttttta tcctggatgt aatgtgccag aagacccggt aacaggatct gctcattgtg
    20641 ttattgctcc ttattggtgt gaacagttaa gcaagaacca tataaccgct ttacaaggag
    20701 gcttcagaca aggtgagctt tattgtgaag tgaagggaaa cagagtatta ctctatggaa
    20761 attgccatct ttatttggaa ggaagaatat atttgccatg aaatatttaa caaccccaag
    20821 gcttttgcta agagcgtggg atgaagaaga tactatcccc ttctatagaa tgagtcagga
    20881 tcctcgggtc atgaagtatt tccccgggtt gtggtctatg gatatggtca aagatttcat
    20941 tatcagaatg aataaacaat tatctcaaaa aaactttact ttgtgggcgg ctgaagtaaa
    21001 ggactgtaag cggtttattg gttttatagg attaaatgct cctacttgga atgctcactt
    21061 cactccttgc gtagagatag gatggcgttt ggcaactgag ttttgggggc aaggctatgc
    21121 tacagaaggg gcaaaagcag ctcttgaata tgcttttctg gagatgcata ttcccgagat
    21181 agtttctttt actgtgcctg ataattcccg ctcaagaagt gttatggata gacttgggat
    21241 gatcagagat attgcgggag actttttaca tcctagatta gcctctgatc acaggttagc
    21301 aaagcatgtt ttatatcgta tacaaaatcc tttaataaaa gtttgatgga ttttgtatat
    21361 gttttatccg gccattagac taaggtctgt tgacatttct actatcttgg ccgaaaagcc
    21421 ttgattttct gtgctccgct acggtgctcg aaaatcaagg cttttcgact caagcgagcg
    21481 aaatatcaac agaccctaat tggcttaata ataactttaa tgccaatgca attaaaatga
    21541 cacctgtaat acgttcaatc caatagctaa ctctttgaaa tttcgtacgc gtattttttc
    21601 cagaaagact gaatgcaaca aaagaaaacc ataacaatgt ttgtagaaaa aggataattg
    21661 ccaggaataa tttattgggg attgaaattt ctctgggcat aagtactgaa aacaaactga
    21721 taaaaaagag catggctttg gggttcaata agttcgttat aaacccattg agaaaagctt
    21781 cacttggttt taaatcgttg tacgatgcat aacttcctat ttggtcagca tttggcttgg
    21841 caccaagatt tttaaggcca atataaaaga ggtatccagc ccctagatat tggatgatat
    21901 gcagtattat aggtgttttg gtaataaaca ggcctaatcc aaggagagtg taactaatat
    21961 gaaccaaact gcccaaagca attcctaacg cggttaataa accactcctt ctggaataag
    22021 ccaagctgtt tttgactgtg atagcaaaat ctggcccagg actgattaat gaaataaaaa
    22081 ctaaaatgat taaagggaat agtagagtaa attgatgcca ttgttccata aagactccaa
    22141 gttttttgct aagttttttc agccaatatc atagctaatt tttaagcgct cttttttaat
    22201 tttacattta gttaataaga cattgtttat cttattttaa ctctctaaat gtacccacca
    22261 caggggtgga aagggtggtt tcctatcctc attaaaggga ttttctctct tt
//
LOCUS       DATNSN010000035        21947 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.035, whole genome shotgun sequence.
ACCESSION   DATNSN010000035 DATNSN010000000
VERSION     DATNSN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21947
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.035"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..938
                     /gene="csy3"
                     /locus_tag="VAH76_001146"
     CDS             <1..938
                     /gene="csy3"
                     /locus_tag="VAH76_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HEO1385790.1"
                     /translation="HKDKATPLRLNEKSVRGTISNRLKPALQKDPAALNNEVEKPNPQ
                     TVDTCSLGSEEDTLKLYFTLKVLGGLKHPSACNNAAFKQSYQRAVSNYLETKGMRELA
                     KRYALNIANARFLWRNRFGAENIQVEVMALNEGAEKSWNFNALDFNPRDLETTTNEVD
                     SLADRIADTLASDNDFLMLQITCYARIGKAQDVYPSQELVLGKGKEKKSKILYAVDGI
                     AAMHSQKIGNALRTIDTWYPEFSDPVSSAGPIAIEPYGAVTNLGKAFRTPADKQDFYT
                     FFDTWARGGELSRVEDEHYMMAVLVRGGVFGESDK"
     gene            949..1503
                     /gene="cas6f"
                     /locus_tag="VAH76_001147"
     CDS             949..1503
                     /gene="cas6f"
                     /locus_tag="VAH76_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HEO1385791.1"
                     /translation="MDHYLDISILPDSEFTTPVLMNAIYTNLHKALHTLASTNIGVSF
                     PKYSSTLGNLLRIHGKKEVLQQLQNLNWIGGMIGYCEASLIKTVPADTKFRTVSRKQP
                     TMSQSKLRRLIKRNSLTEDEIRQYKAKMFSKGLDNPYIELVSVSNGQRHRRYIEFGEL
                     FNEPITGLFDQFGLSNSATVPWFD"
     repeat_region   1735..5362
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1735..1762
                     /rpt_unit_seq="gttcactgccgcacaggcagcttagaaa"
     gene            5414..5911
                     /locus_tag="VAH76_001148"
     CDS             5414..5911
                     /locus_tag="VAH76_001148"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1385792.1"
                     /translation="MGWKLIKELIPDIKSKTAHYEHALLTKGHARPCRVVTTYRQPKG
                     RKVKTSRGKTTSTLSNGSYSQANKEPWILATNLPLDVGPVDIINLYFKKMQIEESFRD
                     VKSHQFGLAGRYLRTHSVNRWGVKMLLAAIVEITYWVIGVIAHSQNKQQYFQANTVKD
                     KKIFS"
     gene            complement(6285..7184)
                     /locus_tag="VAH76_001149"
     CDS             complement(6285..7184)
                     /locus_tag="VAH76_001149"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO1385793.1"
                     /translation="MEKIQRGDTTMGNDTVVSLKKPVVIDDLLTETLRAGARQLLAAA
                     IEAEVAEFLEAHNGTGEKARFVRNGYLLEREIQTGIGQVAVKVPRVRDRSKSSDGIGY
                     VSNLVPKYLRRSKSLNDFLPLLYLKGISTNDFVEVLKPLIGSEGKNLSPGVISRLKAS
                     WEQEYTAWRKREMSGKHYVYFWVDGIYLQVRMEESKACVLVIIGVDERGQKDLIAMEA
                     GYRESKESWLTLLRDLKSRGLHQAPKLAVGDGALGFWGALNEIYPDTRHQRCWFHKMD
                     NILDKLPKSLLSKAKQQLQAIWI"
     gene            complement(7275..7937)
                     /locus_tag="VAH76_001150"
     CDS             complement(7275..7937)
                     /locus_tag="VAH76_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEO1385794.1"
                     /translation="MNIKKEIGKRILEARKAKGLTLKALGELAGGLKQTRLTNWEQGV
                     RTPGPEEIKSLARALDVSPAYLMCLSDDIQIKTVKNPTHLVPLLTHQAFDTQAYNSLV
                     RNQDPSNLMFVSVSTLLLPELSNEAFALKIVNESMTPEIRVSDIIIIDPLTLPKPGDY
                     VVVKVKMQKEVIICQYKKLSYTSSEFELLTLNDTWPNIKVSDCGAVEIIGVVVQNIRN
                     YH"
     gene            8101..8970
                     /locus_tag="VAH76_001151"
     CDS             8101..8970
                     /locus_tag="VAH76_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385795.1"
                     /translation="MMDFVINGDELEAMCGLPHIQQLTYLRGIRPYMDVKTGIVGIKR
                     RISHQSISEQLYVEPHQGIKSQSFSRDQVRRSVSGLVRAGLIEVQSEGMHLILKCLLA
                     SPHYSVQIKAAINPPQKATTKPRWKSLENTGLSEVEALKADTVESQKAAIPHKGNYYI
                     YLLSQFEKFWSLYPEKKSKNNAQAVFEELKPNAELCHTIINALTAQINHRETMKLQGT
                     WVPPWKYPANWLAQRCWEDELPTDALQEKHHAEHTKNTRKRHTTEDLFCPPRDAEDRE
                     HNNVIQLQRYLQS"
     gene            8936..9298
                     /locus_tag="VAH76_001152"
     CDS             8936..9298
                     /locus_tag="VAH76_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HEO1385796.1"
                     /translation="MLSSSKDTFKAERRIDLLFSKFAAFYGHVWRSQFKDEVFLKFAK
                     KEWQEALADFTDAVLIKAILNCREFYELPPTLPQLLYCCRQIRKQESFYVVKDVYQPA
                     NKAVVSSCLQKCKELLAK"
     gene            9311..9514
                     /locus_tag="VAH76_001153"
     CDS             9311..9514
                     /locus_tag="VAH76_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEO1385797.1"
                     /translation="MLVLTRKAGQQILIGKGLIQMKVLKVDDDIISIGIKAPQHIDID
                     REEIYLKKLQQEQAESSMQKVAP"
     gene            9511..9801
                     /locus_tag="VAH76_001154"
     CDS             9511..9801
                     /locus_tag="VAH76_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEO1385798.1"
                     /translation="MNKLKRWSQSLGNLYLIAVCTLLPTLSHAQSIESIINRTINYLQ
                     GGLARTVGVFCIIIAGYLCLARQKFPKEYFVMILVGMGIIFGGSTLYSTLIG"
     gene            9811..10092
                     /locus_tag="VAH76_001155"
     CDS             9811..10092
                     /locus_tag="VAH76_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEO1385799.1"
                     /translation="MSDLEISPIFNALTRPAMTAGVTFEYHMLNLIVSMCAFIGFSPL
                     YGLIFIPLHVFGWLVCRYDTHFFTICAKRFLLPEAPNTSLWRVRAYEPF"
     gene            10079..12559
                     /locus_tag="VAH76_001156"
     CDS             10079..12559
                     /locus_tag="VAH76_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEO1385800.1"
                     /translation="MNLSKTLKHVYQEAKVSDLFPITHLNSPCIFESQSGLVGSVIKV
                     NGAAFEIEEAETLNHQRFLLHQALVSLDSRFIIYVTTHRQKISCPLIGEFKPGFAKDL
                     DERYQQRFKDKNLYKNTLYITVVLKGDDTSKTGSWLKWAKSLSIKGNSELSEHHREQS
                     IQTLNRVVEQLQANLNPFEATILGKQDEVLGFSELLQFLGLVVNAGQTASFKHPVYSP
                     PIANSITQTFKQEVKYPEGHLGQYLSSCQLLFGECIQFQGNTQKDCVFGAMLSLKKYP
                     TNTVSILLDSMLSLDCEFIATHTFAPIGRDSALDTISKKRSKLLSAEDKALSQTTALS
                     DLEDGIASETLLLGAHHHTVMLLAPNKSLLDAAILEATKRYGSAGIVVVKETLGLEPA
                     FWSQLPCNQHFITRASLITSQNFVDFCPLHNTQTGFSNENFLGGAVTLLETPSKTPVY
                     FNYHARGSKTNPSKGHAAIFGGNNAGKTTLVNFLDAQMGRFGGRSFFIDRDESSKIYI
                     LASGNSSYIKIEPSNPIAMNPLQLPDTSENRSFLKSWFATLVQEESERVIPSHIAEII
                     NDCIDYSFEQLAPEFRTLSHLSQFLPIDFPRWPHLKRWLKRNDSRIDGEFHWLFDNQH
                     DALNLDFDKVGFDVTYLMDQVHSVIATPVYLYLLHRMRQCLDGRLTSFIIVEAWQLFA
                     SPFWEKALREWLPTIRKKNGHFIFDTQSPKTITDSPIKHIVLDNLATLIAFPNPLADR
                     ETYMEHLKLTEAQFEAIKENTPESRIFLYKQDHEAFLCKLDLSGLSQYIRVLSANTQS
                     VKLLDDIMQEVGHDPELWLPVFYERSKK"
     gene            12556..13266
                     /locus_tag="VAH76_001157"
     CDS             12556..13266
                     /locus_tag="VAH76_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385801.1"
                     /translation="MKRAACFLIAAMGISSASHADILGVEDAQLILLAMKQLGELKEQ
                     YRVLSDTYQTAKNQLDNLNQLKSMNSGHYGFGDFNNSLDSLQSWQSPVSTWQDALQNL
                     SGGNQQRYQALMEAYEKNHPALDENSYARFTTPANAARFKEDKAVNRAVMVQTTESYN
                     EINKHMEALHKLSQQIEKTPNTKGAIDLNSRLITEIGFIQLMSLRLQALISQQAAQEN
                     LSALQDRAEMAKFNRLPK"
     gene            13281..13427
                     /locus_tag="VAH76_001158"
     CDS             13281..13427
                     /locus_tag="VAH76_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385802.1"
                     /translation="MKWLLSVLMIALLSGCSKPPDLDSPCHHFGQYCPQYPINDEPIM
                     ELTK"
     gene            13424..13819
                     /locus_tag="VAH76_001159"
     CDS             13424..13819
                     /locus_tag="VAH76_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LvrD"
                     /protein_id="HEO1385803.1"
                     /translation="MNTLIKGLMVGVMVATLSACHHENPLKTQPAKASVTFLINASAN
                     AEKRLQFAIHKDAYGFAYLECMEGKQSPDIQCNALYQGMIAFAREGHYSGFQTITQAD
                     LTEQEVFESLADEYAETAATSFPHFVSGS"
     gene            13823..14863
                     /locus_tag="VAH76_001160"
     CDS             13823..14863
                     /locus_tag="VAH76_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEO1385804.1"
                     /translation="MNTLQFDNFIIQLAGEVDKLTNHFVFDGYTALATLLKAPLGAMI
                     VLYIILKGYGIALGVIERPQHELFRFSMRAGLIYMMAMNWDLFSSYMRDLFVSGSESI
                     ATRLMQAVHKYPSGGSINQGLQSVLNEILKLGSDLFDAGSLRKLTPYFAGMMVFLSGS
                     VTIGLAFIEIVIAKLMLAVTLCTAPLFILFTLFDQTKSFFDRWLGVLAGFAFVLIFVS
                     SVVGLCIHLLHWVTYAFLGNTETLTAAIWVPIFIVACLCVMGITQAVAIGKSIGGSVC
                     TSAGSAMVGGFIGGSLGAWGFSKSSVQKPINTLRQGLGKASHLVEKGKQLGEASHNVF
                     KQLHKNLRKGAS"
     gene            14860..15576
                     /locus_tag="VAH76_001161"
     CDS             14860..15576
                     /locus_tag="VAH76_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEO1385805.1"
                     /translation="MSKTNLNDYFKRARSWADDQFGRIEQSRNRYQAAFFSAMGLNVV
                     ALIVIGILAHYQTVVPMLVHHYDNGVTTVEPIENKEIPINRAQIESDIARYIQYRESY
                     DASSYRAQFELVHLLSNSTVAKEYLQEQDAANTASPIHALGNHIKREVRIYSINFLDS
                     VLANEKDLHKDHHALAEVVFSLIDTDKTSGKTTSTHYSAMISWRYTNPPDSPETRWKN
                     WDGFEVTRYSRQTRLMEAQA"
     gene            15573..16325
                     /gene="virB9"
                     /locus_tag="VAH76_001162"
     CDS             15573..16325
                     /gene="virB9"
                     /locus_tag="VAH76_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HEO1385806.1"
                     /translation="MKKILLSAAALLLSLGTYAQNNPMSLPTDTRIKKVVYRENDVIP
                     VHGIPFTTTQIQFAKQERVLDIEGGDTTAWMVTYHPQLENMIFVKPTMFDSNTNMTVI
                     TNQRAYYFHLASTKKLEQEPGKRTYALKFEYPQPQPVHTKNTTPNKTVHPKVVNTAYR
                     FSGSPQLVPRHVFDDGKFTYFELSSQGSVPAIFAVDDQSGKESTVNTRREGKFIVVQR
                     LAPQFTLRQGGLTASVFNAPEINRIKANRRPK"
     gene            16322..17413
                     /locus_tag="VAH76_001163"
     CDS             16322..17413
                     /locus_tag="VAH76_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEO1385807.1"
                     /translation="MSMDDKKREDSLGNRTQVAKNPKHTAIKDKLLLAGVVAICIVIG
                     IGGLFPKQKANTVNEPDEKQSLSMALNQNLELIAALKEKNAKAQSGYKGGDPRHPPLL
                     KTMQTQTVSKETLARMNAPSTFFSSGGNEVISNTTGGEAQASKTLTGRDGNSEFLNQQ
                     NDITSVSAKRLPHPAMTVPAGEMIPATLETAINSELAGMVRAITTRDIFALEGSKLLI
                     PKGSTLVGQFNTAITQSQSRIFVVWNRLQMTNGVIVTLNSPGSDPIGRSGQAADYIDR
                     HFFERFGSGALLSVLGAYTATGGVHGQDEYNSMSQYRMNIASSFQQAANQTLQQDMQT
                     RPTLQINQGAAINVFVAHDLDFYRVAGRA"
     gene            17415..18419
                     /gene="virB11"
                     /locus_tag="VAH76_001164"
     CDS             17415..18419
                     /gene="virB11"
                     /locus_tag="VAH76_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HEO1385808.1"
                     /translation="MEAIKACFSPGATGLLSPLQDFLEDEHVSEILINKPQEVFIERH
                     GQLIRFDIPVLTTQYLRRLFLLIANENKQTLSESSPVLSGNLADGSRVQLVMPPASLH
                     ETLSIRKFTLKQVSFEDYKAKGFFSSARGVGVKEYSECIGKTDGLLELYDAKNWQEFI
                     RQAIMSKKNIIISGGTSSGKTTFLNSCVGQIPLHERLITLEDTYEMDVPHSNIVRLKA
                     LKQIGDHAQKITMQDLVQATLRLRPDRIIMGEIRGRELFDFVSACSTGHSGALATIHA
                     NNPKVAFMRMAQLYKLNQVAGMDEADIYKVLHEVIDVIVQLQKTSDGRRLVEVYYKHA
                     "
     gene            18412..20313
                     /locus_tag="VAH76_001165"
     CDS             18412..20313
                     /locus_tag="VAH76_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO1385809.1"
                     /translation="MRNPRVIAVALIIGLAITAQIAVLLTGITQWSSPLVLIDLIRHG
                     FYWETTIAGCITAVVMGCMAGFLKQGFYRYVSLLILSTLVGVMAIMLLGFELYSWVFL
                     NAFQSPFKVVELATLRYQEPELWRNFLTTEGVAFFLMFLALVIYLFHWLRPDNKALGN
                     AHFSNGFETKRAGFFERQEQSILIGKKYGAPLYSNGFEHVLVFAPTGSGKTRSIGIPN
                     LFHYPYSVVCNDVKLTLFKTTSGYRERMLGHRCFCWAPADENRITHCYNPLSLISEDK
                     IQRLTDIQRIAHILMPDNKKSDPIWQQASRKLFKVAVLYLLDTPERPTTLGEINRLVK
                     QAGFDDWLASVLEETDHLDPEFYRNGYSYLNNHEKTRSSILETFSGYFELFDDPTIDA
                     ATAHSDFDLRQLRREKITIYIGFTDDDMERLSPLLTLFWQQLISVMIKNIPDPVDEPY
                     PLLCLIDEFSSLGRIERLRRSLKLLREYRVRCVLIMQYIAQTYEQYTHDEAKAFTNIK
                     TKIAFATEDIFDAEYVSKLLGTRTVKVSAGSTSTQTQGYSESKSYNYQAIPLLRPDEV
                     MRLPEDQTLIMRTGHAPVKASQMIWYLDPEMKHLACGHTEVPRQIVQHHPFIHRSSDR
                     TALPEAFEL"
     gene            20412..21209
                     /locus_tag="VAH76_001166"
     CDS             20412..21209
                     /locus_tag="VAH76_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEO1385810.1"
                     /translation="MNHLKIIGTAGLFMMSCAAQANDKELWAHIEALYSKIGTLQTQI
                     NSIPQGAPGPMGPAGPKGERGPAGTYTAGEGISISNGEIKTTLTHHIGEEYNGGIIFW
                     VDKTGQHGLIASKTDLNNNEGIQWRNGESGNKVTNARADGIYAGESNTKLIIAQQTID
                     NQSGTFAALLAANYRVLGDGITPCPIPTPESATCYGGWYLPSAYELTLLHNNLQRQGI
                     STFAPDYYWSSTESGVGEAWLQNFSTGELTASGKASTLGHVRAVSRF"
     gene            21489..21824
                     /locus_tag="VAH76_001167"
     CDS             21489..21824
                     /locus_tag="VAH76_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385811.1"
                     /translation="MRKMEFSKMSKCLWALRFEVLGVILLIIATGLTISSGNGLGIVA
                     LFAAGVIFCLFNKFCSYVFSSKQPSCPVCDVSHSYHSEESIQTQEKEENEAKQSMDNE
                     GGNQTNRER"
BASE COUNT         6529 a         4758 c         4646 g         6014 t
ORIGIN      
        1 aacacaagga taaagccacg ccacttcgat taaatgaaaa atctgtgcgg gggacgattt
       61 caaatcgact caaaccggcg ttgcaaaaag accctgccgc actcaataat gaagtcgaga
      121 agcctaatcc tcaaaccgtt gatacttgtt cattgggttc agaagaggac acgcttaaat
      181 tgtattttac gcttaaggtg cttggcggat taaaacatcc ctctgcttgt aataatgctg
      241 cttttaaaca gagttaccag cgggctgtaa gcaattatct tgaaacaaag ggtatgaggg
      301 agttggcaaa acgttatgcg ctgaatatcg ccaatgcccg atttttatgg cgcaatcgct
      361 ttggagcaga aaatattcaa gtggaggtta tggcattaaa cgaaggcgct gaaaaatcct
      421 ggaattttaa tgcacttgat ttcaatccac gtgatttgga aacaaccact aacgaagttg
      481 acagtttggc agatcgtatt gctgacacat tagcaagtga taatgacttt ttaatgcttc
      541 aaatcacttg ttatgccaga attggcaaag ctcaggatgt ttatcccagt caagagttag
      601 tgctgggcaa gggtaaagaa aaaaaaagca aaattcttta tgccgtagat ggtattgcag
      661 caatgcattc gcaaaaaatt ggtaatgctc ttcgaacgat agatacctgg taccctgaat
      721 tcagtgatcc ggtatcttct gctggaccaa tcgcaattga gccatatggt gctgttacca
      781 atttgggaaa agcgtttagg acaccggcag acaagcagga tttctatact ttttttgata
      841 cttgggcacg tggcggagag ttatctcgcg ttgaagatga gcattatatg atggctgttt
      901 tagtgcgtgg tggtgttttt ggtgaaagtg ataagtaggg ggtaggttat ggatcattat
      961 ttagatatat ctattcttcc tgattctgaa tttacaacgc cagtgttaat gaatgcaatt
     1021 tacaccaatc tccacaaagc cttacatact ctggcttcca ctaacatcgg tgttagcttt
     1081 ccaaagtata gctctacttt gggaaacctt ttacgtattc atggtaaaaa agaggttttg
     1141 caacagctgc agaatttgaa ctggataggc ggtatgattg gttattgtga ggcaagcttg
     1201 ataaaaacag ttcctgcgga cacaaaattc cgtacggtgt ctcgtaaaca gcctacaatg
     1261 agccaatcaa aattgaggcg gttaattaaa cgtaactcct tgacagaaga tgaaatcagg
     1321 caatacaagg caaagatgtt ttccaaaggt ctggataatc catatattga acttgtgagc
     1381 gtatcaaacg ggcaaagaca ccgacgctat attgagtttg gcgaattgtt caatgagcca
     1441 attacgggcc tctttgatca gttcggcctt tctaacagtg ctactgttcc atggtttgat
     1501 taaattttag aacaaggtaa attccttgag ttcgataggt taaagtaagg agatatagtt
     1561 tggtcaaatg cttcaatact tgattatttc gatacttcta acgaccaata aatttagttc
     1621 tttaaaaatt tgtcttaaaa tcaattggtt ataaattagc tgaaagatat tgggtaaatc
     1681 gatttttttc agctaacttt ctgttgtaac tattttttta agatttatat tacagttcac
     1741 tgccgcacag gcagcttaga aaaaactgta gataacaaaa caatggttgt tgatgttcac
     1801 tgccgcacag gcagcttaga aataagccag aataatgcta ctcaatgaat accagttcac
     1861 tgccgcacag gcagcttaga aatcagtatg agattgatga taaccccgac tatggttcac
     1921 tgccgcacag gcagcttaga aataatcatg actgggagat atgactagac aaatgttcac
     1981 tgccgcacag gcagcttaga aaattgctct aactacttta tacatgatcg ctagttcact
     2041 gccgcacagg cagcttagaa atgattaaac gctagctctt attactaagt atagttcact
     2101 gccgcacagg cagcttagaa acattgcttc actgcctagt ttactcttag gtgttcactg
     2161 ccgcacaggc agcttagaaa ttccaataga gttgacctag caagatttct atgttcactg
     2221 ccgcacaggc agcttagaaa acaaacacaa actgaattta tcgtgctgta aagttcactg
     2281 ccgcacaggc agcttagaaa acaaacattt ttgaaatatg cagctgtacc acgttcactg
     2341 ccgcacaggc agcttagaaa cattcatgct cacaatagtt atcgatcata gagttcactg
     2401 ccgcacaggc agcttagaaa ccaattttag acgcatattc aatcttttcc ttgttcactg
     2461 ccgcacaggc agcttagaaa tgcaattaca ggcggtggaa atgaagcaga aggttcactg
     2521 ccgcacaggc agcttagaaa acccagcagg taattttata ttatcacgtg acgttcactg
     2581 ccgcacaggc agcttagaaa agtatgaaga tgaaaacata ctggcaatga ttgttcactg
     2641 ccgcacaggc agcttagaaa tgcaatccaa attggccgtg gatatattca gtgttcactg
     2701 ccgcacaggc agcttagaaa atgtgttatg caactggaaa gattaaagcc aggttcactg
     2761 ccgcacaggc agcttagaaa ataatgcgca gtagttcatg ctggtataag ctgttcactg
     2821 ccgcacaggc agcttagaaa aactggttat tgtcttgacc aagatttttt ccgttcactg
     2881 ccgcacaggc agcttagaaa tatggtgagg taagtttgta gtttcataat tcgttcactg
     2941 ccgcacaggc agcttagaaa tttcttgaag gtataacccg cccgtttagg ccgttcactg
     3001 ccgcacaggc agcttagaaa ttgggcaaga atggcaattt aattttaaaa cagttcactg
     3061 ccgcacaggc agcttagaaa aaaatctggc tttggagttt ttccaagtca atgttcactg
     3121 ccgcacaggc agcttagaaa gtatatggac aatttgtata tggatagttt ttgttcactg
     3181 ccgcacaggc agcttagaaa tgaatatacc acaatgagtc gtaagcctgg cagttcactg
     3241 ccgcacaggc agcttagaaa gaatggccac aactgctcca aagtcggaga acgttcactg
     3301 ccgcacaggc agcttagaaa attatcagtc tcctctagca atctacctgt ttgttcactg
     3361 ccgcacaggc agcttagaaa tcattcgcta ggcgtggcga ttgagtttcg tagttcactg
     3421 ccgcacaggc agcttagaaa ttactcggcc acggatgtcg ctcctaatcc cagttcactg
     3481 ccgcacaggc agcttagaaa tattacaaga gctatttcat catcattttt aggttcactg
     3541 ccgcacaggc agcttagaaa gtaacgatta atgtttgtta aatggtgtgc aagttcactg
     3601 ccgcacaggc agcttagaaa acataaaaaa tcttttagga attatattat gagttcactg
     3661 ccgcacaggc agcttagaaa attcaaggtg agatgcctat tgatagaaca tcgttcactg
     3721 ccgcacaggc agcttagaaa aagcacaaag ccattgattc cgtattaacg gggttcactg
     3781 ccgcacaggc agcttagaaa cagcacaagc ccagccgaaa attgctgata tggttcactg
     3841 ccgcacaggc agcttagaaa ttgagcataa atctctttat taagaacagc ttggttcact
     3901 gccgcacagg cagcttagaa attactaaaa ctgacgcgct acgcttgtct gccgttcact
     3961 gccgcacagg cagcttagaa acaacggggc tgatgtatct aaattcattt agagttcact
     4021 gccgcacagg cagcttagaa aataagctat tatactactt aaaaatagta aaggttcact
     4081 gccgcacagg cagcttagaa atgatatgga tagagaagaa tctattcaca caagtttact
     4141 gccgcacagg cagcttagaa aatcaccaaa ccgctttcta agacgcttca taagttcact
     4201 gccgcacagg cagcttagaa atgtatcttc atcaacaaca gcataacgag tcagttcact
     4261 gccgcacagg cagcttagaa atgtagggtt cgcttaaaat atattcatct taatgttcac
     4321 tgccgcacag gcagcttaga aatgatgact ttgctcgtat taaggataat cgtagttcac
     4381 tgccgcacag gcagcttaga aactaaaacg ggctgtaaat ggatagtatt aaatgttcac
     4441 tgccgcacag gcagcttaga aagcgcatgg gcttagcatg tacatttcgt ccatgttcac
     4501 tgccgcacag gcagcttaga aatatgactg gccgtaaatg gatagtatta aatagttcac
     4561 tgccgcacag gcagcttaga aataaataac aaaaatgaca ctcaataaat tcatgttcac
     4621 tgccgcacag gcagcttaga aaaaaaaata aaggctacat ttctgtactt aaaagttcac
     4681 tgccgcacag gcagcttaga aatgtaccta tcttatttcc cgtaagacac aaaagttcac
     4741 tgccgcacag gcagcttaga aactgcccaa ttgaaatcaa cacagcatca tgacgttcac
     4801 tgccgcacag gcagcttaga aaaaattgct taaagatgaa gaggcaacaa aaaagttcac
     4861 tgccgcacag gcagcttaga aatctttttc cttacatgga caatttgtat atgggttcac
     4921 tgccgcacag gcagcttaga aacggcgata cttttaattt gaagatgacc gcttgttcac
     4981 tgccgcacag gcagcttaga aatagatatg atgagttaaa tgtggagtgt taaagttcac
     5041 tgccgcacag gcagcttaga aaaagcgtct gttggtctaa caatcgtata tcctgttcac
     5101 tgccgcacag gcagcttaga aatctttcaa cttgcagtct tttccaaaat tctcgttcac
     5161 tgccgcacag gcagcttaga aatttgtgac tttactttag actgtaaata atttgttcac
     5221 tgccgcacag gcagcttaga aattgagaga tgcgtgccag catgcggtct gagtgttcac
     5281 tgccgcacag gcagcttaga aaaagtaaat ccagaccaga tatacattct tatcgttcac
     5341 tgccgcatag gcagtgggtt ggtattggtt aagtcgtata agaggagtta aatcggtata
     5401 attatccgaa gaactgggat ggaaactcat taaagaacta atccctgata ttaaatctaa
     5461 aacggcacat tatgagcatg cgctattaac caaagggcat gcaagaccct gtagggtagt
     5521 tacaacttat cgtcagccca aagggagaaa agtaaagaca tcacgaggaa aaactacctc
     5581 aacattatcc aatggaagtt atagtcaagc gaataaggag ccttggattt tagctactaa
     5641 tttacccttg gatgtagggc ctgttgatat tatcaatctc tattttaaaa aaatgcaaat
     5701 agaagaatca tttagagacg ttaaaagtca tcaattcgga ctggcggggc ggtatctgcg
     5761 tactcattca gttaatagat ggggcgttaa aatgcttctt gctgctattg tggaaattac
     5821 ttattgggtt attggagtga ttgcccatag tcaaaataaa caacaatact tccaagccaa
     5881 tacagtcaaa gataaaaaaa tattctctta acctcagctc gatataagaa taaggttatg
     5941 tcattgaatt attgaaaaaa ttaccggtgc tatatacatt ttattttttt aaaattcacg
     6001 cctgaattat taaaggaaac aagatgttag caccgatagt agaaatattc tgtgatattg
     6061 atgatttttg caaggtatgg tttaaaaaaa tgtcgcctta tttacttcca ttcccttaat
     6121 aaaaatttag gcttacttat gtcgagctga ggttctctta ttttactctt ggaaaactta
     6181 tgattgagca taatcagctc ggttcgttag cccctgaccc tagaacctta caggaaatta
     6241 tagaacagga gcttaaacgt gtttggtgat ttcatgggga tcagtcagat ccagatggct
     6301 tgcagttgtt gtttcgcctt ggagagtagt gattttggca atttatcgag gatattgtcc
     6361 atcttgtgaa accagcaacg ctgatggcgt gtatcagggt agatttcatt tagagcgccc
     6421 cagaaaccca gtgcgccatc cccaactgca agctttggag cctgatgaag accgcgtgat
     6481 ttcaaatcac gcaagagagt aagccaggat tccttacttt cccgataacc agcttccatg
     6541 gctatgagat ctttttgccc tcgttcatca acaccgataa ttaccaaaac acacgctttg
     6601 ctttcttcca ttctaacctg caaataaatt ccatctaccc agaagtacac ataatgttta
     6661 ccgctcatct ctcgtttacg ccatgcggta tactcttgtt cccagctcgc tttcagacgg
     6721 ctaatcactc cgggagacaa gttttttccc tcactgccaa tcaagggctt taaaacctcg
     6781 acaaaatcat tcgtggaaat ccctttcaga tagagtaacg gtaaaaagtc attcaggctt
     6841 ttgctacgtc ttaaatactt gggaaccaga ttggaaacat agccaatccc atccgatgac
     6901 ttcgaacggt cgcgtacccg tggaacttta accgccactt ggcctattcc tgtctgaatc
     6961 tctcgttcaa gcaaataacc attccgtaca aagcgggctt tttctcccgt cccattatga
     7021 gcttccagaa actctgctac ttctgcttca atcgccgctg ccaataattg ccttgcccca
     7081 gctcttagtg tctctgtcaa taaatcatca atgactactg gtttttttaa tgaaacaacg
     7141 gtatcgtttc ccatcgtggt gtctcctctt tggatttttt ccattaacct tcttcagtta
     7201 atgaggctac accacacaat ctttttttca attatctccg tacacaactt tcaaccgtaa
     7261 ctcgaaaaat atctctaatg ataatttcgg atattttgta caacaacccc aataatctct
     7321 actgctccac aatcactaac tttaatgttt ggccaagtat catttagcgt tagcagctca
     7381 aactcggatg atgtataaga caactttttg tactggcaaa tgataacttc tttttgcatc
     7441 ttcaccttaa ccacaacata gtctccaggt tttggtaaag tcaaagggtc aataattatt
     7501 atatcactca ctcgaatttc tggtgtcata ctttcattaa caatcttaag tgcaaatgct
     7561 tcattgctta gttctggcaa tagtaaagta cttacagaca caaacatcaa attagatgga
     7621 tcttgattac gaaccaggct gttatatgct tgagtgtcaa aagcttgatg agtcaaaagg
     7681 ggaactaaat gagtaggatt ctttactgtt ttaatctgga tgtcatctga caaacacatc
     7741 aaataagccg gcgaaacatc caacgcccgt gcaagcgatt taatctcttc cggtcccgga
     7801 gtacgcacac cctgctccca atttgtcaga cgtgtttgct tcaatccccc ggcaagctcg
     7861 ccaagagcct ttaacgtcaa gcctttagcc ttccttgcct caagaatacg tttgccaatt
     7921 tcctttttga tattcagctc agtcaagttt acccctaaaa attgagaatt aactattgca
     7981 atatcacttt acgtgatata aattagttta taaattcacg attagtgaat aataatcttt
     8041 tatttttata aagatatcac attatggaaa taaaaacgag gatgaatagg gatattaccc
     8101 atgatggatt ttgtaattaa tggtgatgag ttagaggcta tgtgtgggct gccccatatc
     8161 cagcaactga cttatttaag agggatacga ccttatatgg atgttaaaac tggaatagtc
     8221 ggcatcaaaa ggcgcatcag tcatcagtct atttctgagc agctttatgt tgagccgcat
     8281 caaggcataa aaagccagag tttttcgcgt gatcaggttc ggcgttccgt ttcaggattg
     8341 gttcgtgctg gcttaatcga agtgcaatct gaagggatgc acctgatttt aaaatgcctt
     8401 ttggcttcac cgcattattc tgtccaaatt aaagccgcca taaacccgcc acaaaaagcc
     8461 accacaaagc cgcgctggaa atcccttgag aatactgggt tatccgaggt tgaggcacta
     8521 aaagccgaca cagtggaatc gcaaaaagcc gccatacctc ataaaggtaa ttattatatt
     8581 tatttattat cgcaattcga aaaattctgg tcgctttatc ctgagaaaaa atcaaagaac
     8641 aacgcacaag ctgtatttga agagcttaag cctaatgctg aactgtgcca caccatcatc
     8701 aacgcactga cagcccaaat taatcatcgt gaaaccatga aactacaagg cacatgggta
     8761 ccaccttgga aatacccagc gaattggctt gcccaacgtt gttgggagga tgaattacct
     8821 acggatgcat tacaggagaa gcaccatgca gagcatacaa aaaatactcg taaacgtcac
     8881 accacagaag acctgttctg cccaccaagg gatgccgaag accgagagca caacaatgtt
     8941 atccagctcc aaagatacct tcaaagctga gagacgtatt gatttgctgt tctccaaatt
     9001 tgcggctttt tatggccatg tttggagaag tcagtttaag gatgaagtgt ttttgaaatt
     9061 tgccaaaaag gaatggcaag aagcattagc ggattttacc gatgcggtac tgatcaaggc
     9121 gattttaaat tgtcgcgagt tttatgagtt accgcctacg ctgccgcaat tgctgtattg
     9181 ctgcagacaa atcaggaaac aagagtcgtt ttatgtggtc aaggatgtgt atcaacctgc
     9241 aaacaaagcc gttgtgagtt cctgtttgca gaagtgcaag gaacttttag caaaataacc
     9301 ggagaacacc atgttggttt taacacgaaa agcagggcaa cagattttaa taggcaaggg
     9361 cttgattcaa atgaaggtgc tcaaggttga tgatgacatc atcagcattg gcattaaagc
     9421 accccagcat attgatattg accgagaaga gatttatctg aaaaaactcc aacaggagca
     9481 ggctgaatct tccatgcaga aggtggcacc atgaataaat taaaacgatg gagccaatcc
     9541 ctcggcaacc tctatttaat agcggtttgt actttactgc caacgctgtc acatgcccaa
     9601 tcgatagaaa gcatcatcaa cagaaccatc aattaccttc agggtggact tgccagaacc
     9661 gtgggcgttt tttgcatcat catcgcaggt tacctgtgtt tggcacgtca aaaatttcct
     9721 aaagaatatt ttgtgatgat tcttgtaggc atggggatca tctttggcgg atctaccttg
     9781 tattcaacac ttattggata ggagattctt gtgagcgatt tggaaataag ccctatcttc
     9841 aatgcgctga cccgtccggc aatgacggct ggggtgacct ttgaatacca catgttaaac
     9901 ctcattgtct cgatgtgcgc ctttattggg ttctctccac tgtatggtct gatttttatt
     9961 ccattgcatg tttttggatg gttggtgtgt cgatacgaca cccatttttt taccatctgc
    10021 gcaaagcgtt tcttattgcc agaagctccc aatacatcac tatggagggt tcgcgcctat
    10081 gaacctttct aagacattga agcacgttta tcaggaagca aaagtatctg atttgtttcc
    10141 cataacgcat cttaatagcc cctgcatttt tgaatcgcaa tcaggcttgg ttgggtcggt
    10201 gattaaggtg aatggtgcgg cttttgaaat cgaagaagct gagactctaa atcaccaacg
    10261 cttcttgctc catcaggcat tagtgagttt ggattcgcgt ttcattattt atgtgaccac
    10321 gcatcgacaa aaaatatcat gccctttgat tggggaattt aagcctggct ttgccaagga
    10381 tctggatgag cgttatcaac aacgctttaa agataaaaac ctgtataaaa ataccctgta
    10441 catcaccgtt gtgcttaaag gtgatgacac cagtaaaact gggtcatggc ttaagtgggc
    10501 aaaaagtctc tcgattaagg ggaatagtga gctgtccgaa caccataggg aacaaagcat
    10561 ccaaacctta aatcgtgttg ttgagcaatt gcaagctaac ctcaatcctt tcgaggcaac
    10621 gatacttggc aaacaggatg aggtattggg ttttagcgag ctgcttcagt ttcttgggtt
    10681 ggtggtaaat gcaggccaaa ctgcatcatt caaacatcct gtgtacagtc ctcctatagc
    10741 taactcgatc actcagacct tcaaacaaga agtcaaatac cctgaaggac atctgggcca
    10801 atatttaagt agttgtcagc ttttatttgg ggagtgcatc cagtttcagg gcaatactca
    10861 aaaggattgc gtgtttggtg cgatgctgtc actcaaaaaa tatcccacca acactgtcag
    10921 catcctcttg gattccatgt tatcgctgga ttgtgaattt attgccaccc acacttttgc
    10981 ccccattggc cgtgacagtg ccttggatac gatttcaaaa aaacgatcca agctcttaag
    11041 tgctgaggat aaggcattaa gccaaaccac agcattaagt gatttggaag atggcattgc
    11101 cagtgaaacc ctgttactgg gtgcacatca tcatacggtg atgttattgg ctccgaacaa
    11161 atcactattg gatgcagcca tattggaagc caccaaacgc tatggctctg ctggaattgt
    11221 tgtggtgaaa gaaacgctgg gacttgagcc tgctttctgg agccaattac cttgcaacca
    11281 gcattttata acacgagcct ccctgattac ctctcaaaat ttcgtggact tttgcccatt
    11341 acataacacc caaacgggct tttccaatga gaacttttta ggtggcgcgg tgacgcttct
    11401 ggaaactcct tcgaaaacgc cagtttattt taactaccat gcacgaggct ctaaaaccaa
    11461 cccctccaaa ggacatgctg ccattttcgg aggaaataac gcagggaaaa ccacactggt
    11521 gaattttctt gatgcgcaaa tggggcgttt tggtggaaga agttttttta ttgaccggga
    11581 tgaatcctcc aaaatttaca ttttagcttc cggtaacagc tcctatatca aaatagagcc
    11641 atcaaaccca atcgccatga atcctttgca actcccagac acctcagaaa atcgctcctt
    11701 tctgaaatca tggtttgcaa ccttggtgca agaagaaagc gaacgtgtta tcccaagcca
    11761 cattgctgaa atcatcaatg actgtattga ttacagcttt gaacaacttg caccggagtt
    11821 cagaacgctg agtcacttga gccaatttct acctattgat tttccacgtt ggccgcattt
    11881 aaaaagatgg ttaaaacgga atgattccag aattgatggc gaatttcatt ggttgtttga
    11941 caaccagcac gatgccttaa atcttgattt tgataaggtg ggctttgatg tgacctacct
    12001 gatggatcaa gtacacagcg ttattgcaac gcctgtctat ttgtatttgc tccatcgtat
    12061 gcgccaatgc ctcgatggtc ggttaacttc ttttattatc gtcgaagcat ggcagctttt
    12121 tgcatctcct ttttgggaaa aagcacttcg ggaatggctg cctacgattc gaaagaaaaa
    12181 cgggcatttt atttttgata cccaatcccc aaaaacgatt actgattccc ccattaaaca
    12241 cattgtgctg gataacctgg ccaccttgat tgcctttcct aatccgctcg cagacaggga
    12301 aacctatatg gagcatttaa agctgaccga agctcaattt gaggcgatta aagagaacac
    12361 gcctgaatca cgtatttttc tttataaaca agatcacgaa gcctttttat gcaaactgga
    12421 tttaagcggt ttaagccaat acatcagagt gctttctgcc aatacccaat ccgttaagtt
    12481 gctggatgac atcatgcaag aggtcggtca tgacccagaa ctttggcttc ctgtctttta
    12541 tgaaaggagc aaaaaatgaa acgagccgcc tgtttcttaa ttgcagcgat gggcatcagc
    12601 agtgccagtc atgcagatat tctgggtgtg gaggacgcac agttaatttt attggcgatg
    12661 aaacaattag gtgagctcaa agagcaatac cgtgttttat ccgacaccta ccaaacagcg
    12721 aaaaaccaac ttgataacct gaatcaactt aaatccatga actctggaca ttatggattt
    12781 ggagatttta ataatagtct cgattcatta caaagctggc aatcccctgt aagcacttgg
    12841 caggatgcgt tgcaaaacct ctctggtggc aatcaacaac gatatcaggc actcatggag
    12901 gcttacgaaa agaaccatcc cgcactggat gaaaacagtt atgcgcgttt cactactcct
    12961 gccaatgccg ctcgctttaa agaagataag gccgtcaatc gtgcggtgat ggtgcaaacc
    13021 accgaaagct ataacgaaat caacaagcat atggaggcgc ttcataagct ctcgcaacaa
    13081 attgaaaaaa cacccaacac caagggagct attgatttaa attcccgcct cattaccgaa
    13141 atcgggttta ttcaattaat gagcttaaga cttcaggcgt taatcagcca gcaagcagca
    13201 caggaaaatt tatctgcgct ccaagacagg gctgaaatgg caaagtttaa tcgattaccc
    13261 aagtgacaag gagtaagcac atgaaatggc ttttgagtgt cttgatgatt gcattactaa
    13321 gtggctgttc aaagccccct gatttggatt ctccctgcca tcactttggg cagtattgcc
    13381 ctcagtatcc cattaacgat gaacccatta tggaactcac aaaatgaaca cactcataaa
    13441 aggtctaatg gttggtgtaa tggttgcaac actaagtgcc tgtcatcacg aaaacccgtt
    13501 aaaaacacaa cctgccaagg ccagtgttac gtttttgatt aacgcctcgg ccaatgctga
    13561 aaagcgattg cagtttgcta ttcacaagga tgcttatggc tttgcctatc tggaatgtat
    13621 ggaaggcaag caaagtccag acattcaatg taatgcgcta taccaaggca tgattgcctt
    13681 tgccagggaa ggtcattatt cgggcttcca aacgataaca caggctgatt taaccgagca
    13741 agaagtgttt gaatcactgg cagatgaata tgccgaaaca gcagccacca gctttcctca
    13801 ttttgtttcg ggaagttaaa tcatgaatac actacagttt gacaatttca tcatacagct
    13861 tgcaggtgaa gttgataagc tgaccaacca ttttgttttt gatggttata cagctcttgc
    13921 aacactgctt aaagctcctt tgggagcgat gattgttctt tacatcattt tgaaaggcta
    13981 tggaattgct ctgggagtta ttgagcgacc ccagcatgaa ttgtttcgat tcagtatgcg
    14041 ggcgggactt atttacatga tggctatgaa ctgggatctg ttttcatcct acatgcgcga
    14101 tctatttgta tcgggtagcg agtccattgc tacccgattg atgcaggcgg ttcacaaata
    14161 cccatcaggc ggttcaatta atcaggggtt acaaagcgta ttgaatgaaa tcctcaagct
    14221 gggttcggat ttatttgatg caggatcact cagaaaactc acgccctatt ttgcgggcat
    14281 gatggtgttt ttatcaggga gtgtcacgat aggtctggct tttattgaaa tcgtcatcgc
    14341 taaactgatg ttggctgtga ccttatgcac tgcaccctta ttcatcctgt ttacgctttt
    14401 tgaccaaacc aaatcctttt ttgaccgatg gcttggcgtg ctcgccggat ttgcctttgt
    14461 gcttattttt gtgtccagcg tggtggggct ttgcatccac ttgttgcatt gggtgactta
    14521 tgccttttta ggcaatacgg aaacgcttac ggctgccatt tgggtaccaa tttttattgt
    14581 ggcctgttta tgtgtcatgg gcatcaccca agccgttgct attggaaaaa gtattggtgg
    14641 ttcggtgtgc acttcggctg gctctgccat ggtgggaggc tttattggtg gcagtttagg
    14701 ggcatgggga ttttcgaagt cctccgttca aaagccaata aatactctgc gccaaggatt
    14761 aggcaaggca tcgcatctcg tggaaaaggg caaacaactg ggggaagcca gtcacaatgt
    14821 attcaagcaa ctgcataaaa acctgcgaaa aggagcatca tgagtaaaac caatttgaac
    14881 gattacttta agcgtgcgcg aagttgggca gacgaccagt ttggccgaat tgagcaatca
    14941 cgaaaccgat atcaagccgc atttttctca gccatgggtt taaatgttgt agctctcatc
    15001 gtgataggga tactcgcaca ttatcaaacc gtagtaccga tgctggtgca tcattatgac
    15061 aatggggtta caaccgttga acccatagaa aacaaagaaa tccccataaa tcgcgcacag
    15121 attgaaagcg atatcgcccg ttatattcaa taccgcgaat cctatgatgc atcgagctat
    15181 cgcgctcaat ttgaattggt gcacctgtta tccaatagca cggttgccaa ggaatatctg
    15241 caggaacagg atgctgcaaa taccgcctca cccatccatg ccttggggaa tcatatcaag
    15301 cgagaagtgc gcatttacag cattaatttt cttgattccg ttttggcgaa tgaaaaagac
    15361 cttcacaaag atcaccacgc cttggcagaa gtggttttta gtctcataga caccgacaaa
    15421 accagtggta aaaccacctc tacacattac agcgccatga tttcatggcg atacaccaat
    15481 cccccggatt cccctgaaac ccgttggaaa aactgggatg gatttgaagt cacacgctac
    15541 tcaagacaaa cccgtttgat ggaggctcaa gcatgaaaaa aatactgttg agtgcagccg
    15601 ctctgctctt gtcgttaggc acctatgccc aaaataaccc aatgtcttta ccaaccgaca
    15661 cccgaatcaa gaaggtggtt tatcgggaaa acgatgtgat accagttcat ggtatcccct
    15721 ttaccacaac gcaaattcaa tttgcaaagc aagagcgggt tcttgatatt gaaggtggcg
    15781 acaccaccgc atggatggtg acctatcatc cacaactgga aaatatgatt tttgtaaaac
    15841 ccacgatgtt tgattccaat accaatatga cggtgattac caatcaacgc gcctattatt
    15901 ttcatttggc cagcactaaa aaactggaac aggagccggg taaaagaacc tatgctttga
    15961 aatttgaata ccctcaacca caacccgttc acacaaaaaa tacgactccc aataaaacgg
    16021 tacatccaaa agtagtcaac accgcgtatc gatttagtgg cagcccgcag cttgtccctc
    16081 gccatgtatt tgatgacggg aagttcacct attttgagct aagtagccag ggtagcgttc
    16141 cagcgatttt tgcggtggat gaccaaagcg gaaaggaatc aaccgttaat acaaggcgtg
    16201 aagggaaatt cattgtcgta cagcgtttag ccccgcaatt tactttaagg caaggtggcc
    16261 ttacggcatc ggtctttaac gctccggaaa tcaatcgtat caaagcaaac aggaggccaa
    16321 aatgagtatg gatgataaaa agcgggaaga ttctttgggc aatcggactc aggttgctaa
    16381 aaatccaaaa cacacagcaa tcaaagacaa gttattgctc gcaggcgttg tagcgatttg
    16441 tattgtcatc ggcattggcg ggttattccc taagcaaaaa gcaaatacag taaatgaacc
    16501 agatgaaaaa caaagtttaa gcatggccct aaaccaaaat ctggaactga ttgcagccct
    16561 taaggagaag aatgccaaag cgcagtcggg ttataaaggt ggcgacccaa ggcatcctcc
    16621 tttacttaaa accatgcaaa cccaaacggt tagcaaggaa actctagctc gaatgaatgc
    16681 accctccacc ttttttagct caggcggtaa tgaggtgata agtaatacaa cgggtggcga
    16741 agctcaagcc agcaaaaccc tgacaggccg tgatggaaat tctgagtttt tgaatcagca
    16801 aaatgacatc acttcagttt ctgcaaaacg attaccgcat cctgcgatga ctgtacctgc
    16861 aggtgaaatg attccagcga cactcgaaac ggccattaat tcggaactgg cgggtatggt
    16921 gcgggcaatt acaacacggg acatctttgc tttggaaggc agcaagttat taatccctaa
    16981 aggctccacg ctagttggac aatttaatac cgcaattacc caaagccaga gccggatatt
    17041 tgtcgtatgg aaccgtctgc agatgaccaa cggcgtaatt gtgaccttaa atagccccgg
    17101 cagtgatcca ataggtcgtt caggacaagc ggccgactac atagaccgcc atttctttga
    17161 gcgttttggc agtggtgcgc ttttgtctgt tttgggtgcg tataccgcaa caggtggtgt
    17221 tcatggtcag gacgaataca actccatgtc acaataccgc atgaacattg cgagcagttt
    17281 ccaacaagct gcaaatcaaa cactacagca agacatgcaa acacgcccga ctttgcaaat
    17341 caatcaggga gctgccatta acgtatttgt agctcacgat cttgatttct accgagtggc
    17401 tgggagggca taatatggaa gctattaaag cctgcttctc gcccggcgca acaggtctgc
    17461 tctcgccatt gcaggatttt ctggaagatg agcacgtctc tgaaatttta atcaataagc
    17521 ctcaagaggt ttttatcgag cgacacgggc aattaattcg atttgatatt ccggtattaa
    17581 ccacccaata cctgcgccgt ttgtttttac tgattgccaa tgaaaataag caaacgctct
    17641 cggaatcctc gcccgtgctt tctgggaatc tggctgatgg ctcaagggtt caactggtga
    17701 tgccaccagc ctccctacat gaaacactat ccatccgaaa atttacttta aaacaggtta
    17761 gcttcgaaga ttacaaagcc aaaggatttt tttcatcggc gcgtggggta ggagttaaag
    17821 agtactcgga gtgcattggt aaaaccgatg ggctgcttga actctacgat gctaaaaact
    17881 ggcaggaatt tataagacaa gccattatga gtaagaaaaa catcatcata tcgggtggaa
    17941 cctcttcagg taaaacgact tttttaaaca gttgtgtagg gcaaataccg cttcatgaac
    18001 gcctcattac acttgaggat acttatgaaa tggatgtacc ccacagcaat attgttcgtc
    18061 tgaaagcatt aaagcaaatc ggggatcacg cacagaagat caccatgcag gatttggttc
    18121 aagccacctt aagactcaga cccgaccgca ttattatggg tgaaattcgc gggcgagaac
    18181 tctttgattt tgtatcagcc tgttccacgg ggcatagcgg tgcacttgca accatccatg
    18241 ccaataaccc aaaagttgct tttatgcgta tggcgcaact ctacaaactg aatcaggttg
    18301 caggcatgga tgaggctgat atttacaaag tgttgcatga agtcattgat gtcatcgtcc
    18361 agttacaaaa aacatcagac ggcaggcgat tagtggaggt ctattataaa catgcgtaat
    18421 ccacgggtta ttgcagtcgc cttaatcatc gggcttgcca taaccgccca aattgcagtg
    18481 ctgttgacag gtatcaccca gtggtcaagt ccattggtgc ttattgattt aatcagacat
    18541 ggattctatt gggaaaccac aatcgcaggt tgtatcaccg ctgttgtgat gggttgcatg
    18601 gctggctttt tgaaacaggg attttatcga tacgtttccc ttttaatctt aagcaccttg
    18661 gtgggtgtaa tggccattat gctattggga tttgaactct atagctgggt ttttttaaat
    18721 gcgttccaaa gtcctttcaa agtagtggag ctggctaccc ttcgatatca agaacctgaa
    18781 ctatggcgaa actttctgac tactgaaggg gttgcattct tcctgatgtt tttagcgctt
    18841 gtgatttacc tgtttcattg gctcaggcca gataacaagg cactgggtaa tgcccatttc
    18901 tcaaatggct ttgaaaccaa acgcgcagga ttttttgagc gtcaggaaca aagcatactg
    18961 attggtaaaa aatacggtgc gcctttgtat tcaaacggct ttgaacatgt tctggttttt
    19021 gcacccaccg gaagcggtaa aacacgaagc atcggcatac ccaatctgtt tcactacccc
    19081 tactcagtag tgtgcaatga tgtaaaactc acgcttttta aaacaacctc aggataccgt
    19141 gagcgtatgt tagggcatcg atgcttttgc tgggcacctg ctgatgagaa ccgaatcacg
    19201 cattgttaca atcccttatc cctaatctct gaggataaga ttcaacgcct aacggacatt
    19261 cagcgcatag cccatattct aatgccagat aataaaaagt ccgaccccat ttggcagcaa
    19321 gcctcacgta aattatttaa agtggccgtg ctgtacctgc tggatacgcc tgaaagaccc
    19381 accacccttg gcgaaataaa ccgcttggtc aaacaggccg gatttgatga ttggctggcc
    19441 tcagtcttgg aggaaaccga tcatctcgac ccggagtttt accgcaatgg ctattcctat
    19501 ttgaataatc atgaaaagac gagaagctcg attcttgaaa cgttttccgg ctactttgaa
    19561 ttattcgatg atcccactat tgatgccgcc acagcgcatt ctgattttga tttacgtcag
    19621 ttaagacgag aaaaaataac gatttacatc ggttttaccg atgatgacat ggaacgactc
    19681 tcaccgttgt taacactgtt ctggcagcag cttatttctg tcatgattaa aaacatcccc
    19741 gacccagtgg atgaacccta tcctctgtta tgcctgattg atgagttttc atcattaggc
    19801 cgtattgaaa ggctaaggcg ttcacttaaa ttattgcgcg aataccgtgt gcgttgtgta
    19861 ctcatcatgc aatacatagc ccaaacctat gaacaataca cccatgatga agccaaagcc
    19921 tttaccaaca ttaaaactaa aatagctttt gctaccgaag acatctttga tgcggagtat
    19981 gtcagtaaat tgttaggtac acgcaccgtg aaagtatccg caggctccac cagtacccaa
    20041 acccaaggtt attccgagtc taaaagctat aactatcagg ccatcccttt attaagaccg
    20101 gatgaagtca tgcgtttacc cgaagatcaa accctgatta tgcgaacagg tcatgcaccc
    20161 gttaaggctt cgcaaatgat ttggtatctg gatcctgaga tgaagcattt ggcctgcggt
    20221 cataccgaag taccgcgcca aatcgttcag catcatccat ttatccatcg ttcatcagat
    20281 agaacggcac tgcccgaagc attcgaacta taaaccacca caggagaaaa ccaaatcagc
    20341 caacaaaact aaaaggatgc tctgaaaaga gattatggat tcatacagac ttaattgagg
    20401 taagaatcag aatgaatcat cttaaaatta taggtacagc agggcttttc atgatgtcat
    20461 gtgcagctca ggcaaatgac aaggaacttt gggctcatat tgaggcgtta tacagtaaaa
    20521 ttggtacttt gcaaacgcaa attaactcga taccccaagg tgcacccggt ccaatgggtc
    20581 cagcaggacc aaaaggggag cgaggacctg ccggaaccta tacagcagga gaaggcattt
    20641 cgatcagcaa tggtgaaata aaaacgactc taacccacca cattggcgaa gaatataacg
    20701 ggggcattat cttctgggtc gataaaacag gacagcatgg attaattgct tccaaaactg
    20761 acctcaataa caatgaaggg atacaatggc gaaacggcga aagtggcaat aaagtaacca
    20821 atgcccgcgc tgatgggatt tacgcaggcg aatccaatac caaactcatc attgcccaac
    20881 aaaccataga caaccaatca ggtacttttg cagcacttct ggctgcgaat tatcgcgtgc
    20941 taggcgatgg catcacgcct tgcccaatcc cgactccaga atcagctact tgttacgggg
    21001 gatggtatct accttcggcc tatgaattga ccttgcttca taataatttg cagcgtcagg
    21061 gcattagcac ttttgcgcca gactattatt ggagttcgac agaatctggt gtgggtgagg
    21121 cgtggttaca gaatttttct acaggggagt taacggctag tggcaaagct agtactttgg
    21181 ggcatgttag ggctgtgagt cggttttagt ttattaatgt gcttatcttc acagtgaaga
    21241 taagcacatt aataacaata taattttagt caccagaagg acacttttta ctgcattcta
    21301 actccctaat ttccaaaaga ctgatttgga aaaaaagatt caacggcaaa agtatcccca
    21361 gttccgttac gccatcattt ttcatctcaa ctatacataa aaatacgaag aatcaaatag
    21421 tagtctaaaa ttaatgaaag ttattttttc cgctaatatt gatacatcaa tagtatagga
    21481 gtaataccat gagaaaaatg gaattttcta aaatgagcaa atgtctctgg gctctgcgct
    21541 ttgaagtgct gggagttatc ttattgatta tcgcaactgg tttgaccata tcttctggga
    21601 atggccttgg aatagtcgct ttatttgctg caggtgtcat cttttgttta tttaataaat
    21661 tttgtagtta cgttttctca tcaaaacaac catcctgccc tgtttgtgat gtatctcatt
    21721 cttatcactc tgaagagtcc attcaaactc aggaaaagga agaaaatgaa gcaaaacaat
    21781 ccatggataa tgaaggtgga aatcaaacta acagggaaag atagcctatt catatattaa
    21841 agaaagtatt actttcaatt gcctagtctt taattgcgat tattcatcta tttgggaggg
    21901 aattcaaaaa agctgtgttc aaaattacaa ttatctaaca aactaat
//
LOCUS       DATNSN010000036        21787 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.036, whole genome shotgun sequence.
ACCESSION   DATNSN010000036 DATNSN010000000
VERSION     DATNSN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21787
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.036"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            175..795
                     /gene="sspA"
                     /locus_tag="VAH76_001168"
     CDS             175..795
                     /gene="sspA"
                     /locus_tag="VAH76_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HEO1385812.1"
                     /translation="MAIVAKRTIMSLFSDVDDVYSHQVRIVLAEKGVNVEILAVKQDE
                     PGADLLSINPYGTVPTLIDRELVLYEARIIMEYLDERFPHPPLLPVYPVARAEARKMM
                     HRIEQDWYYLMNAILREESVEQARANLLESLTSLDPVFADKPYFLSDEFSLLDCALAP
                     LLWRLPKLGLEIAPNFKGIISYMQRVFKRDSFQTSLTDAERQIRAA"
     gene            798..1193
                     /locus_tag="VAH76_001169"
     CDS             798..1193
                     /locus_tag="VAH76_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEO1385813.1"
                     /translation="MAMTSNKPYLIRAFYDWIVDNDLTPYILVDASNPNVQVPREHVQ
                     HDRIVLNISPSATRGLLLENDRIVFTARFSGQTEQIFVAPNAVLEIYAKENGRGIAFS
                     VDEDDEPPPKTSSSSESATKNKPSLKLVK"
     gene            1321..2802
                     /locus_tag="VAH76_001170"
     CDS             1321..2802
                     /locus_tag="VAH76_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HEO1385814.1"
                     /translation="MNKLENALYRCDQIKACEQQAATLYQKDENALMALAGAEAFSFL
                     QKIYPHVRHIAVFCGAGNNAGDGYVLARLAHEQGFSVTVYQCKSIEDLPSAARNAARL
                     ALTSGVHCQSADEPLDGDAELIVDALLGIGLQGAVHGVIANAIHQINASGLPVLSIDI
                     PSGLNADTGLVENICVNATATITFIGLKTGMYTLDGPDYCGVIHCAHLNLEACRTKLT
                     RFASLLRDSPLPLPLAARKKNSHKGDYGHVLVIGGGPGMPGAVSLAAKAALRTGAGVV
                     TVATWPDYAKSALALIPEAMIWGVKTAAELMPLLAKATVCVVGPGLGDSDWAKTLFLT
                     AITTQLPMIIDASALRLLSQHPQMDDNWILTPHPGEAASLLSCTVRDVQTDRYHAASA
                     IQKQYGGVVVLKGVGTVIQSAERNIFVCPQGNPGMATAGMGDILSGIIAAFCAQGFTL
                     SDAAKLGVWVHAVAGDKVAEKSGEVGILASDLLDEIPSILNCI"
     gene            2929..3411
                     /gene="tsaE"
                     /locus_tag="VAH76_001171"
     CDS             2929..3411
                     /gene="tsaE"
                     /locus_tag="VAH76_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO1385815.1"
                     /translation="MTIEDNDNFITLDLVSEQESKRFAEKLAFCISAPLVITLSGDIG
                     AGKTTIIRAMLKSLGVISAIKSPTFSLVESYNCGQFHIHHFDLYRIHQEEELEYLGFR
                     DYFSNQSVCCIEWPEHGGKTLPPVDIQFNLIIKGTGRLIQIAALSVAGKRILSCLAGE
                     "
     gene            3411..4841
                     /locus_tag="VAH76_001172"
     CDS             3411..4841
                     /locus_tag="VAH76_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO1385816.1"
                     /translation="MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQ
                     GSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDV
                     KQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISS
                     QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAI
                     TLKLKRLIDRQPGMRAVLTRSGDYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNG
                     ASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVRSVLIDLSQTATINA
                     GLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNP
                     AYQERLSQAIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVSA
                     LQSINRLQARARLMPGQKLVIPGTWS"
     gene            4832..6562
                     /gene="mutL"
                     /locus_tag="VAH76_001173"
     CDS             4832..6562
                     /gene="mutL"
                     /locus_tag="VAH76_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO1385817.1"
                     /translation="MVLRIHQLSPAIANQIAAGEVIERPASVVKELLENSLDAGASVI
                     GVDVNYGGLLQITVSDNGSGIVGDDLPLAVAAHATSKIRTLDDLYSIDSMGFRGEALA
                     SIASVAKVTIISKPEEQDNAMMLRVEGENRTLSPCARNIGTTIDVSDLFYNAPVRKRF
                     LKNEKLEFQAIEMVVKRFALSAPRIALTLKHNKKLIFSLPPALSEQAKAIRMGRILGN
                     TFMREAIFLDVEHSGMRLYGWISNQSLQRSQNDRQWIYVNQRMVKDKLLQHAVKQAYD
                     GLLHAGRFPICLLYFSLPASEVDVNVHPTKHEVRFQQPRLVHDFFTSQLTKALQSTLD
                     HSDMREYAYAASSCNEQKNTVSEPSHICNDRESHEGEKALFPSWSGNSLSSSITSKRY
                     KKNSSVYHPETESQWVILNERFILVFIKQQPYLADIVALFQEWIEKQLMQKALPLESR
                     PLLISIRYSLPNQWIHKPEQLKHELARVGIQIEYSNADDLLIRSIPLNAPYLDVRRFF
                     NILCELDFFDTEQLIALISRSQIFDPKQLTVEERIEMNQLLLELSLKEEKHNICKALT
                     IDNCRMLLHE"
     gene            6555..7496
                     /gene="miaA"
                     /locus_tag="VAH76_001174"
     CDS             6555..7496
                     /gene="miaA"
                     /locus_tag="VAH76_001174"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO1385818.1"
                     /translation="MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMN
                     IGTAKPSIHELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTM
                     MYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
                     IQRALEVYYLTGSTLSTYLTGPHEQPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEG
                     FIEEVQQLQAKWPIQINLPAMRCVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQL
                     TWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN"
     gene            7519..7713
                     /locus_tag="VAH76_001175"
     CDS             7519..7713
                     /locus_tag="VAH76_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEO1385819.1"
                     /translation="MRKTTKEPASAKKNYVVHRHQLPLSCPTDEMELWNAHPKVYLPI
                     EKTGVEVCPYCGSRFVLQND"
     gene            7757..8743
                     /locus_tag="VAH76_001176"
     CDS             7757..8743
                     /locus_tag="VAH76_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEO1385820.1"
                     /translation="MLVPLIRTLQNKLPNAQLTWIISKPAFYLVEGMEGVEFIVIDKP
                     KTLADYWRFRKQMLHRQFDVLLAPQASFRTNWLIPFIKAKRKVGYDSHRANDGHRLFI
                     KESITPGLEHTLEGFLKFAEPLGVTEKIIHWDLPITKADYEWAQNHLPEQERPILVVN
                     PAASKPERSWLAERYIEVLKQAQVKWDVKVVLTGGPGDHDKSLAEEIAKEIPVVNLVG
                     KTKPKQLLAVISKAKAVLCPDTGPSHMAAAVGTPVVALHAVTNPCISGPYTFQHLVVN
                     RYSQAIQTVLGVSPEKNIWGTQVHGYEAMKLITVDDVMEKLAEIFSGLMNNT"
     gene            8832..9689
                     /gene="legD1"
                     /locus_tag="VAH76_001177"
     CDS             8832..9689
                     /gene="legD1"
                     /locus_tag="VAH76_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegD1"
                     /protein_id="HEO1385821.1"
                     /translation="MILIFEEHRMQLSSEQCDFFFTNGYLVINNFFSESVCDLLKQRI
                     ETLLENNQAEIPKTIFSTQTNEHAKKQYFLDSGDKIHYFFEPGAFDEAGDCLRPFNQS
                     INKIGHALHELDPVFRQYSRDDRIKKIAHQLGLKTLGLVQSMYIFKQPGIGAEVLCHQ
                     DSTYIFGEDSDALGFWFAIEDATLENGCLEVIPSPCTTPLKQRMFRRENEIYFENFDS
                     SPWPEENSVPLPVKKGALIILHGRVPHKSQANFSNQSRHAYTLHLVDISLPYPEKNWL
                     QWPNGIPCL"
     gene            9813..10613
                     /locus_tag="VAH76_001178"
     CDS             9813..10613
                     /locus_tag="VAH76_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1385822.1"
                     /translation="MDNESLLAILNNWSATNYAKGNYILREISSKFLAKISIHSNDYL
                     LDAGCGDGSFTQMLANLVPDGYVLGLDRSKTMIDYANKHCRTINVRFDIGDIQEPIIY
                     GPFDNILSFWCLHWTDLEKSLSNLYHVLKPGGKICAVFSSGKQSTLFEVLNSLQFQNL
                     YPDVAKNVVQSRMMRENYSVRCRRALDNLPFKSISFVVEHCQVELPDLGLFENFIHGA
                     PLFKDLPGKMYQDIVNSMVQFFDEYCKKKYGGKWVYETTPYFLTITRE"
     gene            complement(10685..11215)
                     /locus_tag="VAH76_001179"
     CDS             complement(10685..11215)
                     /locus_tag="VAH76_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2692 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385823.1"
                     /translation="MPTTFEDKSSKVPSLKSLAANAVQKTNANLFFRLHSLETPPEIK
                     KGFIDKELEALTRELTEDYQTKVEARNEKIEECSSNLSSNECFVKCSAFTITTLMAGV
                     HVGIYYILKAAAVDPSTQIAYISSIPATICFSMCVGVCLNRQITKCLSSCFTPSVPDK
                     ITVDLDELGRQSHVSP"
     gene            complement(11466..14009)
                     /locus_tag="VAH76_001180"
     CDS             complement(11466..14009)
                     /locus_tag="VAH76_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEO1385824.1"
                     /translation="MSKPYFFYVSGMNCTSCSNAIESFLRNSNLIKINTFHADMSTPD
                     PKKIVISLSEEKENEHESNWKTIKKLIDDTGFTCENYEFPQMKDQKPALPLEKTQTTV
                     QQEATQLSALFSSAKKIISSHWLLGGLGCITGVSLMILTMAIGGLPLALMIPLAGLST
                     LLTIALGAQSYYEAWKKLVHSRTLTMDTLFSISTISVLIVSLASFFVPWLPMMFEAGL
                     LIYGFRHIGIAIEDTIKDKISSAKFQDRAPQKVKLYRHPDLKKTRIEEIQPNDIIQVK
                     PGELVPLDSVCEQDTLIYPTIITGAVIPKQFRSGEKILAGMRLANDAKPLTLRVLKTA
                     KESYLARLDESIAQSVLEKAPIEVKTGQLLSYFIPSIIALSILSGIIVGLIATPALAI
                     QCAISVLVSACPCTLGLIIPLAVKTGIHKGAEHGVQFKNSRVLQLAEQIDVVIFDLNG
                     TLTTGVPVVKEYAVLNDADLPSEQFLSICNVLEEQSKHPFGKAIQSFTQGINKQKFNI
                     ANLDHSNHSGVSAEINGIQYTIGSKTLMQEIGISTSSLENQMNLVAGDSLVFLARNKQ
                     LLGYLVITDPLRKDAKQTVDALIKMGKEVHLCTGADEKTAYRYAQALGIKKVHANCVA
                     TTINTEDRSKPVYIKSLQNQGLKVAMIGDAANDANALAASDFGIAVLSQDSDEITQQH
                     ASAIIQSGTILPIANAFAISKQTVNNINQNLLISLGYNLTTVLIAGGLLVALGFVLNP
                     AVGVALMVTQACFVLLNVYRFKEQPLQHLQDKVQEKPEPAPSSYEAMKKHSPNFHTAP
                     EINHSQAIQSGKQPTNKNKLPFFWSDYFPELFEEEKDTSTEHLMNNKIG"
     gene            14364..16004
                     /locus_tag="VAH76_001181"
     CDS             14364..16004
                     /locus_tag="VAH76_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HEO1385825.1"
                     /translation="MSSLLSRRKTILVLLIAIYCAYDLMVSLYTAWDFTTDDAYISWY
                     YARQLVSDKGLFWHVTLPPVEGYSNFLWLMIAALVIKLQLPLVSSMKVISCCSLGLAL
                     LFLYRLARLFCSPLLSILPVFLFSHYTGVTWWTVSGLETLFYCALSLALIWQCTLAFG
                     YQTVGDRPWPNRFSLYSTTHWIITNMLLLLLSLTRFEGIVWIIPLAVFIGCQYLRAQR
                     ELVFKDHKRLFLWVSIIFICFVLPYFAYFIWRLIYFGHWIPNSYRCKALAYGQFLAVD
                     FDYFLFITPLLVLSIPYLLSANKDCRHVLLWVPSVLYSLLLWKANPVVTYGLRLFLSP
                     FALFSILPVLGLNQLIQYFKPFRWDPEYLAAVLMLFVTCFFVSGNDLHHLSKAVTEYN
                     ERTLNRMTIADLLNRKAKKGDSVLLDDCGIIPFFSREDIRFIDAQCLNNSDLTQKPFK
                     NDLSLYADYLNSEVKPDWVITNYYPMESRGDYLTDLLYEKHFFKNYHLISTLSSGFYL
                     NKNQPQGKKMIDFVYQIFKRVNKPGYNGSLELESQEPK"
     gene            complement(16008..17417)
                     /gene="icmX"
                     /locus_tag="VAH76_001182"
     CDS             complement(16008..17417)
                     /gene="icmX"
                     /locus_tag="VAH76_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmX"
                     /protein_id="HEO1385826.1"
                     /translation="MKVLPKLALANLFCFTTLSVFAQQGYDTYDQQTSKNTNSLVTYL
                     TNLGKYLGYDITEKGKPPTPPYSQLFNSNVAQLIQNYAYNTFLGAIPVDAMSQSLMNF
                     VTNKVQGNALINNLANSTFKYQNFSSPSSGSDGKITANSLIDQSTTSAQAPTPSGVFS
                     AIQIPTSGTYLNDPVSQAVFNILGTPDYSFCMDNEQKNWLPNCNLMYQNLVMQNIIGT
                     LPPALPNQSSLEPFYSYTYNKPLISQLNSNSLIAPLLLDTSAAQSEQKSGLKAQSQAQ
                     QALNFIRYASAQVTPPSLPKLSAYTELWNQAKAEPGSQGYNEVQQKQAAATLSGYFNN
                     LRVYAAQTSVGVSNLYYILSKRLPQNMSGDQNSSNITSQALNEFNMATRRLFDPTASN
                     TPGQPNQQWIKQINDASPATVQKEIAVLLAEINYQMYLDRQIQERILLTNSIMLLQNL
                     KAAQPTADFSNQSSPSEQQ"
     gene            complement(17417..17872)
                     /gene="icmW"
                     /locus_tag="VAH76_001183"
     CDS             complement(17417..17872)
                     /gene="icmW"
                     /locus_tag="VAH76_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmW"
                     /protein_id="HEO1385827.1"
                     /translation="MPDLSHEASAKYWFEYLDPMIYRVITFMESVENWTLDGNPELED
                     AMKQLGQELDDIEKIDLGLLAEEDKFIRIVGNIKSGRGLRLLQAIDTVHPGSASRVLI
                     HAEETSLSSNDPAGFFLKRNIVFERLRLLSRVFCQYRLKLVLRALEGDE"
     gene            17985..18440
                     /gene="icmV"
                     /locus_tag="VAH76_001184"
     CDS             17985..18440
                     /gene="icmV"
                     /locus_tag="VAH76_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmV"
                     /protein_id="HEO1385828.1"
                     /translation="MKKKSGSRIVRVITRIINVRKWVDWDRMKSFTLYLVNGGKRLFV
                     PQEPTHVESFDEAAKKLKLSEADLIIKQKALFRLSVIMVIAAFMLLIYTGYQLFYGSW
                     KATIISLVVVMIALVLAFRYHFWYYQIKQRKLGCTVKEWYRQGLLGEKE"
     gene            18437..21556
                     /gene="dotA"
                     /locus_tag="VAH76_001185"
     CDS             18437..21556
                     /gene="dotA"
                     /locus_tag="VAH76_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotA"
                     /protein_id="HEO1385829.1"
                     /translation="MNKLAITVLLCFFPALALAESGALSFAPPASDLSVVFLGNLFGV
                     VDGVLHGTGSQIMGNMFGVFNSAVLALGGIIIMYTLMVSTMNTAHEGQMLGQKWSSIW
                     IPLRSTFGLALLIPKASGYCMMQVFFMWVIVQGVGAADKIWEAALSYLNRGGVIIQAQ
                     SDPTKSLTDKGGSSTGIANGALTILAGQVCMLGLQKQLQAQRDMYLSQSKSPPCGGNP
                     STEMDKFCRTAIPDFISTVNFVKKQNDSKPEGPFSIDMPNFEQGSTFDFLNGICGTVK
                     WKSISGLSANMGKGINLNSSELKTAQLSRAIAIQQMYVTLSTVAQVMVNNDPAFSTTS
                     STGNSKNDFSDIAKQQFGVPYKASGQPCEIYSDVCQTWGAATSSSGSTPGVLFNGTEF
                     LGAINDYNGIMTPTVNLLKQKDALADDDTSRKFIAEANAKGWIMAGSYFFDLVKLNGS
                     AVKDDADFDSVTGLEDSNFDLEQLTKPFGKTCQGTRALLCTWFQNKSDKLVQIQSLIN
                     GVPALSQDGVKQPDLKPNTGRKAVEGLLSSTVYGFVNNSMMVQLPGQPGIKPLTFANL
                     INFKVDTSLYYMKHQDFDCGRVKILFFSFCLGRMMGDLFYNYVFRYVYNFFLAIFGEM
                     INSIVMAFLMIPLQGMKDIFIVGVQTLTKPGVNPIVALANMGTMYINFSGTLWLTLLN
                     MAVVSSLIPLFGIFIFALIMMAMPLLMAWIGTMVSIGFVTAYYIPVLPYMIFTFGSFA
                     WLIAVIEAMVAAPIVALGVTHPEGNEAFGKGEFAIMILVNVFLRPSLMIIGYITAIAL
                     SYVGVWILNAGFDHAISFIQSDQGIETWEKGDFSKTHADSAYCKRYPADCKIAEAGQT
                     VWNWSGTKGDTSQWNDFGSKLSGDYQSTALQSDTLNHGYTGWAGVYAFFFSILIYTTM
                     YLIIVQKAFTLIAHLPDKVLRWIGGSPESFGQETMQWGEEAKGKVEKAGEATYGAEKA
                     IGDKLGAKGQEMLGKFGPQAKQISENAGDVSGKGKNSGSQTGPSSKPDTGQLGGSDSG
                     TPTSTPPPE"
BASE COUNT         6248 a         3922 c         4776 g         6841 t
ORIGIN      
        1 ttaccttcag aatcagaaaa tatatctggt ttcttgtatc gactttacgc taaaataaga
       61 agatttgctc aaagtaaccc agaagtttaa aaatgtgata gaatagcaac ctttgtacac
      121 tagaatataa aaagccattt taacaggctt aaatgaattg ataggagtaa ggtaatggca
      181 atcgttgcaa agcgcaccat aatgtcttta ttttctgatg tagatgatgt ttatagtcac
      241 caagttcgta tcgttctggc agaaaaagga gttaatgttg aaattcttgc agtaaagcaa
      301 gatgagcctg gagccgactt attgtctatt aatccttatg gaaccgttcc aacattaatt
      361 gatagggaac ttgtattata tgaagcaaga atcattatgg aatatttgga cgaacgtttc
      421 cctcatcctc cattattacc agtttatcca gtagcccgtg ctgaagctcg caaaatgatg
      481 catagaattg aacaggattg gtattattta atgaacgcta ttttgcggga ggaatctgta
      541 gagcaagcca gagctaattt acttgaaagt ttaaccagtt tagatcctgt ctttgcagat
      601 aaaccttatt ttttaagtga tgagttttcg cttttggatt gtgcattggc tcctttatta
      661 tggcggttac ctaaattagg tttggaaatt gcgcctaatt ttaaaggaat aatttcctat
      721 atgcagcgtg tgtttaaacg tgattctttt caaacgagtt tgacggacgc tgagagacaa
      781 ataagagcag cataattatg gcaatgacat caaacaaacc ctacttgatt cgtgctttct
      841 acgattggat tgttgataat gatttaacac cctacattct cgtagatgca tcaaatccca
      901 atgtgcaggt tccaagggaa catgtccagc atgatagaat tgtattaaac atttctccct
      961 cagcgacccg aggactttta ttggaaaatg atcgcattgt gtttacggca cgcttttctg
     1021 ggcaaacaga gcaaattttt gttgctccca atgctgtact agagatttat gcaaaagaaa
     1081 atggcagagg aattgctttt tcagttgacg aagatgatga gccaccacct aaaacttctt
     1141 cttcctctga aagtgctaca aaaaataagc catcactaaa attggtaaag tagacggcat
     1201 ggtttctttt aagcgaatcg tagccaagaa gcactgattt ttacagcatg atcttggcat
     1261 agcgatataa aagtattttg tggtcgttat atcaaattta cgttaatttg gagccgagag
     1321 gtgaacaagc tagaaaatgc tctgtatcgt tgtgatcaaa ttaaagcttg tgaacagcaa
     1381 gctgcaactt tgtatcaaaa agacgaaaat gccctgatgg ctctggctgg cgcagaggca
     1441 ttttctttct tacagaaaat ctacccgcat gtacggcata tagccgtctt ctgtggtgct
     1501 ggcaataatg caggtgatgg ttatgttttg gccagattag ctcatgaaca aggattctca
     1561 gttactgtat atcaatgtaa atcaatagaa gatttacctt ccgctgcccg gaatgccgca
     1621 cgcctggcat tgacatctgg agtacattgt caatccgcag atgaaccttt agatggcgat
     1681 gccgaattaa ttgttgatgc attattgggt attggattac aaggagcagt gcatggggta
     1741 atagccaacg cgattcatca aatcaatgcc agtgggttac cggtattatc gattgatatt
     1801 ccatctggtc ttaatgcgga tacaggatta gtagagaata tttgcgtcaa tgcgactgcc
     1861 acaattactt ttatcggact taaaacagga atgtatactt tggacggacc agattattgt
     1921 ggtgtgattc attgtgctca tttaaacctg gaggcatgca gaaccaaatt aacccgattt
     1981 gcttccctgc tgagggattc tcccttgcca ttgcctctcg cagctagaaa aaagaactct
     2041 cataaaggtg attatggaca tgttttagtg ataggcggag gtccaggaat gccgggagca
     2101 gtgagcttgg cagccaaggc ggcactcagg acgggagcag gagtagttac tgttgcaacc
     2161 tggccggatt atgcgaagag cgctttagcc ttaattcctg aagcgatgat ctggggtgtc
     2221 aaaacagcag cggagttaat gcctttatta gcaaaagcta cggtttgtgt tgttgggcct
     2281 gggcttggtg atagtgattg ggccaaaacc ttgtttttaa ccgccattac aacccaacta
     2341 cccatgatca ttgatgcttc agcgctgaga ttactttctc aacatcccca aatggatgac
     2401 aattggattt taacacccca tcctggcgaa gctgccagtt tgctttcatg tactgtaaga
     2461 gatgttcaaa cggatagata ccatgcggca tctgctatac aaaaacaata tggtggtgtt
     2521 gtggtactga aaggagtagg cacggttatt cagtcagcag aaaggaatat ttttgtgtgc
     2581 cctcaaggca atccaggtat ggctaccgca ggcatggggg atattttgag tgggattatt
     2641 gccgcttttt gcgcccaggg ttttacctta tccgatgctg cgaagttggg cgtgtgggtc
     2701 catgctgttg ctggagacaa ggttgctgaa aaatcaggag aagttgggat attggcgagc
     2761 gacttacttg atgaaattcc aagcattttg aattgtatct agtgcgatga gtcattaaga
     2821 aggtaaacgg aactgttact taggtaacaa actctgtttc aatgcttgtt tataacggct
     2881 tggctgacag gctttttcta aacagataca ttatgtagtg ttctcgatat gactattgaa
     2941 gataatgata atttcataac tttggatttg gttagtgaac aagagagcaa aagatttgca
     3001 gaaaaattgg cgttttgtat ctcagcgcct ttggtgatta ctttaagtgg tgatataggg
     3061 gccggaaaga caaccattat tcgcgcaatg ctaaaatcac tcggtgtaat ttcagccatc
     3121 aagagtccaa ctttttcatt agttgaaagt tacaattgtg gtcaatttca tattcatcac
     3181 tttgatttgt accgcattca ccaggaagaa gaactggagt atttaggttt tagagactat
     3241 ttttccaatc agagcgtttg ttgtattgaa tggcctgaac atggtggaaa aacattgcct
     3301 ccagtcgata ttcagtttaa tttaataatt aaagggactg ggcgcttaat acaaatagct
     3361 gcattaagtg tcgcgggtaa aagaatttta tcttgcctgg cgggtgaatg atgatgatgc
     3421 gagcgtgggt cttaagtctg ttattatatc taccaagttc ttttgctgta ttttctgcac
     3481 aattaaagaa tattacagtt tctcagcaac caaccatgac gtctctttat ctttctatac
     3541 aaggttcatt tacccataag ctatttacct taagtcagcc caatcgcgtg gttttagacc
     3601 taagtgatac acaattagcc gtcaatatca gtcaattggg tttaatcaat tcgcttattc
     3661 agcaggtaag aagcggtagt ccctccaaaa acacgctcag gcttgtcatg gatgtaaaac
     3721 aaccagtcaa ggtgcgctca tcttcatgga aaccgaaagg agaatttaat gggatcagaa
     3781 ttgatcttgt taatgatact cctttagtca taaataatcg caggcagttg agctcgcctg
     3841 ttaaaacgat taaagctcct gtccagcaaa tccaatcaaa aatatccagt cagccatcag
     3901 aacaacgaat aactgtgcat aaaacacctg tccaggttcc tcataaccca tcaaaaaaac
     3961 ctttgcgtga tgttgttgta gtgttggatg cagggcatgg gggcaaagat cctggtgcaa
     4021 gaggtccgcg ccgcagtaac gagaaggatg ttgtgctggc cattacttta aaacttaaac
     4081 gattgataga caggcaacca ggtatgcgcg cagtcctgac acgatcggga gattattacg
     4141 ttggacttag acaacgcttg gatattgcca gaaaatataa tggtgatgta ttcatttcta
     4201 tccatgccga tgcattcaat aatcctcact ctaatggagc ctcggtattt gccttgtcac
     4261 aaaggggggc caccagtgaa gcagcacgct ggttagcaga aaaggagaat tactctgaac
     4321 taggtggagt gaatttaggt gagttggatg atagtaatgg ggtagtacgc tctgttttga
     4381 tcgatttatc acaaactgcg actataaatg ctggtttgga aatgggggga aaagtattag
     4441 gtcaattgga taattttacc aatttgcata ataacaaagt ggagcaagcc cgttttgttg
     4501 ttctgaaatc tccagatatt ccttctattt tggttgaaac cggttttatc tcaaatcccc
     4561 gtgaggaaag aaatttaacc aaccctgcct atcaggagcg attgagtcag gcaatatttc
     4621 aaggactaaa aaattatttt tgggatcatc ctcctcatgg gactcgaatt gaggctatga
     4681 tttctccaaa ttttcatatc gttaaaaagg gtgagactct tcctgctatc gctgctcgat
     4741 atagggtatc tgtgagcgca ttacaatcaa ttaaccgtct tcaggcaagg gcaaggctaa
     4801 tgcccgggca aaaattagtg atacctggca catggtctta aggatacatc aactttcgcc
     4861 agcaatagcc aatcagattg cagcaggaga agttatagaa agacctgctt cggtagtcaa
     4921 ggagctgctg gaaaactctt tggatgctgg tgccagtgtg attggtgtcg atgtcaatta
     4981 tgggggattg ctgcagatta cagtaagtga taatggttca ggaatcgttg gagacgactt
     5041 gcctttagct gttgcagctc atgcgaccag taaaatcaga acgcttgatg atttatattc
     5101 cattgatagt atgggcttta gaggggaggc attggcaagt attgcctctg ttgccaaagt
     5161 gactatcatt tcaaagcctg aggagcagga caatgcgatg atgttgcgag tagaggggga
     5221 gaacaggact ctctctcctt gtgcgcgcaa tattggcact accatcgatg tttctgattt
     5281 attctataat gcgccagttc gtaagcggtt tttaaaaaat gaaaaactgg aatttcaagc
     5341 tattgaaatg gttgtgaaac ggtttgcatt aagtgctccc cgaattgcat taacgcttaa
     5401 gcataataag aaactgattt tttcattacc gccagcgtta agtgagcaag ccaaagccat
     5461 tcgtatgggt cgaattttgg gcaatacttt catgagagaa gctatttttc ttgatgtgga
     5521 gcacagcggt atgcgtcttt atggctggat aagtaaccag agtttgcaac gcagccagaa
     5581 tgataggcag tggatttatg tgaatcaacg catggtaaaa gataaattac ttcaacatgc
     5641 agtaaaacaa gcttatgatg gcttattgca tgcaggcagg tttccaattt gtttgttgta
     5701 tttttctcta cctgcttctg aagtggatgt gaatgtccat cctacaaagc atgaagtgag
     5761 attccaacag ccccgcctgg tgcatgattt ttttacttcc caattgacga aagcgttgca
     5821 atcgactctt gaccattcag atatgaggga atacgcatat gctgcttcct cttgtaatga
     5881 gcagaaaaat acagtttcgg aaccttccca tatctgcaat gacagagagt cccatgaagg
     5941 ggaaaaagct ttattcccaa gctggagtgg caattcatta tcatcatcta taaccagcaa
     6001 aaggtataaa aaaaacagct ctgtatatca tccagaaaca gagtctcagt gggttatctt
     6061 aaatgagcgg tttattttag tttttataaa gcaacagcct taccttgcag acattgtagc
     6121 actgtttcaa gaatggatag aaaaacagct aatgcaaaag gcattgcctc tggagagcag
     6181 gcctttattg atttcaattc gttactcatt accaaaccaa tggatacata agccggagca
     6241 attaaagcat gagttggctc gtgttgggat acaaattgag tattctaatg ctgatgattt
     6301 gttaattcgc agcatccctt taaatgcgcc ctatttagat gttcgtcgtt tttttaatat
     6361 tctctgcgaa ctggattttt ttgatactga gcaattgata gcgttaatta gccgctctca
     6421 aatatttgac ccaaaacaat taacagttga agagcgtatt gaaatgaatc aattgttgct
     6481 ggaactctct cttaaagaag agaaacataa tatatgtaaa gcgttaacta tagataattg
     6541 tcggatgtta ttgcatgaat aagctagtgt tttgtttaat gggaccaact gcttcaggaa
     6601 agacagggtt agcatgcgag ttgctaactc atttcccttt tgaaattatt agtgttgatt
     6661 ccgccatgat ttatagagat atgaatattg ggacagcaaa accttcaatt catgaattgc
     6721 agagagcgcc tcattattta attgatatta aagatcctgt tgaatcctat tcggcagccc
     6781 agttttgtac agacgctttg tctttgtgtg ctgaaatcat caaaagaggg aatattcctt
     6841 tgttagtggg tggaactatg atgtatttta atgctctgca aaaagggtta gccactttgc
     6901 ctgaagccga tgaagctgtt cgtaaacgat tggaggagga agcattatca cagggttggg
     6961 attttcttta tcaaaagcta agtcaattag atccggtaac agccgctcgc attcatgctc
     7021 atgatacgca aagaattcaa agagcactgg aagtttatta cttaaccggt tcaacgttat
     7081 caacctattt gaccggaccc catgaacagc ctgattatta ttttgttaat cttgctcttt
     7141 ttcctgagca aagatcctgg ttgcatgaac gtatcgcgca acggtttgac gcgatgctta
     7201 gtgagggatt catagaagaa gttcaacaat tgcaagcaaa atggcctatt caaatcaact
     7261 tgcccgccat gcgatgtgtt ggatataggc aaatactcga gtacctcgca ggccattatg
     7321 attatgagac tatgagggaa aaaggtatag cagcaacaag gcagctggcc aaacggcaat
     7381 taacctggtt gaggcattgg gagggcgctt tattttatga ttcgcaaaat gtgggtttta
     7441 atacagatat tatagcgaaa ataagggaaa tattagataa tactgtctct aattgacatc
     7501 atgccgcaaa ggaacaacat gaggaaaaca acaaaagaac ctgcaagtgc taaaaaaaat
     7561 tatgtcgtac atagacatca attaccattg agttgcccaa cagatgagat ggagttatgg
     7621 aatgctcatc ccaaggttta tttgcctata gagaaaaccg gggtggaggt ttgcccttat
     7681 tgtggctctc gttttgtatt gcagaatgat taaatcgatt tgtgttataa ggctttcagc
     7741 tttaggggat gttttgatgt tggtcccact gataaggacc ttgcaaaata aattgcctaa
     7801 tgctcaattg acctggatta tttcaaaacc tgccttttat ttggtggaag gaatggaagg
     7861 cgtggagttt attgttattg ataaacctaa aacgcttgct gactattggc ggtttaggaa
     7921 acaaatgttg catcgccagt ttgatgtgtt gttagcgcct caagccagtt ttagaactaa
     7981 ttggcttatt ccatttatta aagctaaacg caaagttggc tatgattctc acagagcgaa
     8041 tgatggccac cgtctgttta taaaggaaag cattactcct ggattggaac acacacttga
     8101 gggcttttta aaatttgctg agcctttagg tgtcactgaa aaaataatcc actgggattt
     8161 accgattacc aaggcagatt atgaatgggc tcaaaatcat ttgccggaac aagaaaggcc
     8221 aattttagtt gttaatcccg cagccagtaa acctgagcgc agttggttag ctgagcgata
     8281 cattgaggtt ctgaaacagg cgcaagttaa atgggatgtc aaggttgttt taactggagg
     8341 ccctggtgat catgataaat cattggcaga ggaaatagcg aaagaaattc cagtggttaa
     8401 tttggttgga aagaccaaac ctaagcaatt gttggctgtg atcagtaaag caaaagcagt
     8461 cttgtgtcct gatacaggtc cttctcatat ggcggcagca gtaggcacgc ctgttgtggc
     8521 tttgcatgct gttaccaacc cctgtatttc tggcccgtac acttttcaac atttggtagt
     8581 taacaggtac tcgcaagcta tccaaactgt attgggagtg agtccagaaa aaaatatttg
     8641 gggtacgcag gtccatggat atgaagccat gaaattaatt accgtagatg atgtaatgga
     8701 aaaattagct gaaatatttt caggacttat gaacaatacc taatctttgt gcgggaaagg
     8761 tttttaatga gaacatttcg cttatgctca ttaaaagcgg tttgccctta cccaggtgat
     8821 ttccccaagt attgattttg atttttgagg agcataggat gcaactttct tctgagcaat
     8881 gtgatttttt tttcacaaat ggatatttgg taataaataa tttcttttca gaatctgtat
     8941 gcgatctatt aaagcagcgc attgaaacat tgcttgaaaa taatcaggca gagataccta
     9001 aaacaatttt ttccacgcaa accaacgagc atgcaaaaaa acaatatttc cttgattctg
     9061 gagataaaat tcattatttt tttgagccgg gtgcttttga tgaagccggt gattgtctac
     9121 gtccttttaa tcaatcaatt aataaaattg gacacgcgtt acatgaactg gatcctgttt
     9181 ttaggcaata ttcccgagat gatcgaatta agaagatagc acatcagctt gggttgaaaa
     9241 cacttggatt ggtacaatcg atgtacatat tcaaacagcc gggaatagga gctgaagtac
     9301 tttgtcatca ggatagtacc tatattttcg gagaggatag tgatgcttta gggttttggt
     9361 ttgcaataga ggatgctact ttggaaaatg gctgtttgga agtgattcca agcccttgta
     9421 ccaccccctt aaagcagagg atgtttcgtc gagaaaacga aatttatttt gaaaatttcg
     9481 attcaagtcc ttggccagaa gaaaacagtg tccctttacc tgttaaaaaa ggcgccttaa
     9541 ttatactaca tggcagagtt ccacataaaa gccaggctaa tttttctaat caatccagac
     9601 atgcctatac tttgcatttg gttgatattt cattacctta tcctgagaaa aattggttgc
     9661 aatggcctaa tgggattcct tgtttataac atcactagtt tagagatctc agagaataca
     9721 acatcaaaag cttctgattt ttagaaaatt gtgctggaga actctataat ttacaagagg
     9781 ctcttggtaa atcattttgg aagaaatccc gaatggataa tgaaagtttg cttgccatat
     9841 tgaataattg gtctgcaacc aattatgcaa aaggtaacta tatcctaaga gaaatctcca
     9901 gcaagtttct tgcaaagata tctatccata gtaatgatta cttactcgat gctggttgtg
     9961 gtgatggcag tttcactcaa atgctagcca atttagtccc tgatggatat gttcttggct
    10021 tggatcgatc aaaaacaatg attgattatg caaacaaaca ttgcaggact atcaacgttc
    10081 gttttgatat aggggatatt caagaaccta taatttacgg gccatttgat aatattttat
    10141 cattttggtg tcttcactgg actgatttgg aaaaatcatt atccaattta tatcatgtct
    10201 tgaagccagg aggtaaaatt tgtgcagttt tttcttctgg caaacaaagc actttgtttg
    10261 aagtgttaaa cagcctgcaa tttcaaaatc tttaccctga tgtcgctaaa aatgtcgtgc
    10321 aatctagaat gatgcgggaa aattattctg ttcgatgccg aagggcattg gataatttgc
    10381 catttaaaag catttctttt gttgtagaac attgccaagt agaattacct gatctcgggt
    10441 tatttgaaaa ctttatacat ggcgccccat tattcaagga tctccccggt aaaatgtatc
    10501 aagatattgt aaacagcatg gtgcagtttt ttgatgagta ctgcaaaaaa aaatacggtg
    10561 gaaaatgggt gtatgaaacc actccctatt ttttaacaat taccagggag tgaagaatca
    10621 ttgtaattaa attatatttt agcgaataag tcggtttttt ctttcaagga acccataaaa
    10681 ccgcttatgg actaacatgg gattgtctac ccaactcatc caaatcgaca gtaattttat
    10741 cggggacact gggcgtaaaa caagagctca agcatttggt aatttgtcga ttcaagcaga
    10801 ctccaacgca catagagaaa caaatagtag cgggaatact cgaaatatat gctatttgcg
    10861 ttgatggatc gacagctgct gctttcagga tgtaataaat accaacgtgc actccagcca
    10921 tcaatgtagt gatggtaaaa gcagagcatt ttacaaaaca ttcattagaa ctaagattgg
    10981 agctgcattc ctcaattttt tcgttcctgg cttccacctt ggtttgatag tcttcagtta
    11041 attcgcgggt taatgcctct agctccttgt caataaaccc tttttttatc tcagggggag
    11101 tttcaagtga gtgcaacctg aaaaagagat ttgcattcgt tttttgaacg gcattagcgg
    11161 ccagggattt taaactgggg actttggatg atttatcttc aaaggttgtt ggcatgagtt
    11221 ttcttaaaat gtaagttaat taattttgcc aatttgacca tataatttat ttattgtcca
    11281 tataatgatg cggtatacat tgctgattac ctcatttcac atgcgatttt tatgagtcat
    11341 catatattgg taagagacat cacattcttg accctttaga gcataataaa tcaaaataga
    11401 ttgttatgat tgaggaaggt gattagcctt cctcatttca aattcagcca agctttattt
    11461 agggcttatc ctattttgtt attcattagg tgttcggtac ttgtgtcctt ttcttcctca
    11521 aataattcag gaaaataatc actccaaaag aaaggtaatt tatttttatt ggttggttgc
    11581 tttccagatt gaatggcttg cgaatgatta atctcgggag ctgtatggaa attgggtgaa
    11641 tgctttttca tggcttcata agaagaagga gccggttctg gtttttcctg aactttgtct
    11701 tgcaaatgtt gcaagggttg ctctttaaag cggtatacat tgagcaagac aaaacaagcc
    11761 tgggttacca ttaatgccac accaacagca gggtttagca caaaaccaag ggcgactagc
    11821 agaccgccgg ctattaatac tgttgtcaaa ttgtacccta agctaattaa caaattctgg
    11881 ttgatattgt ttactgtttg ttttgatatg gcaaaggcat tggctatagg caatatagtt
    11941 ccactttgta taatggctga ggcgtgttgc tgggtgattt catcgctgtc ttgagacagt
    12001 acagctatac cgaagtcact cgcagcaagg gcgtttgcgt cattagccgc atctccaatc
    12061 atagctactt ttaatccctg attttgtaat gatttaatat aaaccggttt ggatctatcc
    12121 tctgtattga tagttgtagc gacacaatta gcatgtacct tcttaatgcc aagagcttgc
    12181 gcatagcgat aggcggtttt ttcatctgca ccagtgcata gatgcacttc ttttcccatt
    12241 ttaattaaag catcaaccgt ttgctttgca tcttttcgta aggggtcggt gatgaccaga
    12301 taacctaata gttgtttgtt tctggctaga aacaccaggc tatcgcccgc aaccagattc
    12361 atttggtttt caagcgaaga tgtagaaatc cctatttcct gcattaaggt tttactgcca
    12421 atagtatatt gaataccatt aatctctgct gaaacgcctg aatggttcga atgatccaaa
    12481 ttagcaatat tgaatttttg tttattgatt ccttgggtaa acgattgtat tgctttgccg
    12541 aaagggtgtt ttgattgttc ttccaacaca ttgcaaatac tcagaaattg ctcagaagga
    12601 agatcagcat catttaatac agcatattct ttaaccacag gaacgccggt tgttaaagtg
    12661 ccattcaagt caaaaatgac tacatcaatt tgttctgcta attgtaatac cctggaattt
    12721 ttaaactgca caccgtgttc ggctccttta tgaattccag tttttactgc tagaggaata
    12781 attaaaccaa gcgtacaagg acaagcagaa accaacactg aaatagcgca ttgtatcgca
    12841 agtgctggtg tggcaatcaa gccgacaatg atccctgaaa ggatggagag agcaataatg
    12901 gatgggataa aatagctaag aagctgccct gtttttacct ctatcggtgc cttttcaagt
    12961 acagattggg ctatgctttc atcaagacgg gcaaggtatg actcttttgc agtttttaga
    13021 acgcggaggg tgagtggttt tgcatcattt gccagacgca ttccggcaag tattttttca
    13081 ccactacgaa attgtttagg tatgacagca ccggtaatga tggtgggata aataagggtg
    13141 tcttgctcac aaacgctatc caatggtaca agctcacctg gtttgacttg tataatgtcg
    13201 ttgggctgaa tctcttcaat cctcgttttt tttaaatcag gatggcgata taatttgact
    13261 ttttgtgggg ctctgtcttg aaatttggct gagcttattt tgtctttgat agtgtcctca
    13321 atagcgatac caatatgcct gaatccataa ataagtaatc ctgcctcaaa catcataggt
    13381 aaccacggta caaagaatga agcaagcgac acaataagaa cagaaatagt gctaattgaa
    13441 aacaatgtat ccattgtgag tgtgcgagag tggactaact ttttccaagc ctcgtaataa
    13501 gattgggcac caagcgcaat agttaataaa gtgctgaggc ccgctaaagg aatcattagc
    13561 gcaagaggta aaccacctat cgccatagtc aggatcatta agctaactcc ggtgatacaa
    13621 cccaatcctc caagcaacca atgggagctg attatttttt ttgcgctgct aaagagtgca
    13681 gataattggg tggcttcctg ttgaacggtt gtctgcgttt tctcgagagg aagagcgggt
    13741 ttttgatctt tcatttgagg gaattcataa ttttcgcaag taaaacctgt gtcatcaatt
    13801 aattttttaa tggttttcca gttgctttca tgttcatttt ccttttcttc ggaaagagat
    13861 atcacaattt ttttgggatc cggagtgctc atgtccgcat gaaatgtatt gattttgatt
    13921 aaattggagt ttcttaaaaa ggactctata gcattgctgc aactggtgca attcattccg
    13981 ctgacataaa aaaaataagg tttggacata atagctcgct gagttaaatg gttaactcat
    14041 cctgagaact ttgtaccgga tttatcaatc aacaccgacc attgagtagg ggttttaaat
    14101 caaggcctac catcttgagt ccagtggatg cgattcactg ttttgaaatg ttattgtggt
    14161 actcccgtat cacaatgccc cagacttttg gcgtttcttg taagttccgt attattccat
    14221 tgaggataca gtttttttaa attgggttgg aatcataaca tacccattat tttttttgca
    14281 tcatgatttt ggagcttgtt gatactttat gatattgcta ttgtggattg tgttataaaa
    14341 aaatttctgg agcagaacac attttgagtt ccctgttatc aagaagaaaa accattctgg
    14401 tgctactgat agccatttat tgcgcctacg acctgatggt ctcgttgtat acagcttggg
    14461 atttcacaac agatgatgct tatatcagct ggtattatgc acgacagtta gtgagtgaca
    14521 aagggctgtt ttggcatgtg accctgcctc ccgtagaggg gtattccaat tttttatggc
    14581 ttatgattgc tgccttggtt attaaattgc aattacctct ggtgagcagt atgaaggtca
    14641 tttcctgttg tagcctggga ttggcattac tgtttttata tcgactggca agactttttt
    14701 gtagccctct gttatccata ttaccggttt ttttatttag ccattataca ggagtgactt
    14761 ggtggacggt cagtggatta gaaaccttat tttactgcgc tctgtctttg gcattgatat
    14821 ggcagtgtac cttggcattc ggttatcaaa ctgttggtga caggccatgg cccaatcgat
    14881 tttcccttta ctcaaccact cattggataa taaccaatat gctcttgtta ctattgagct
    14941 taacacgatt tgagggaatc gtttggataa ttcctttggc tgtttttata ggatgtcaat
    15001 atttgagagc tcaaagggag ctggttttta aagatcataa gagattattc ttatgggttt
    15061 caattatttt tatttgcttt gtattgccct attttgcgta ttttatttgg cgattaattt
    15121 atttcggcca ttggattcct aatagctatc gttgcaaagc gttagcttat gggcagtttt
    15181 tggcagtaga ttttgattat tttcttttta taacaccatt acttgtattg tcgataccct
    15241 atttattatc tgccaataaa gattgtcgcc atgttttgct ttgggtgcct tctgttttat
    15301 atagtttgtt gctttggaaa gcaaatcctg tagtcacata cggtttgaga ctgtttttaa
    15361 gcccatttgc tcttttttcc atattacctg ttttagggct taatcagttg atacaatatt
    15421 ttaaaccttt cagatgggat cctgaatatc tggctgctgt attaatgtta tttgttacct
    15481 gtttttttgt gtcggggaat gatttacatc atttatccaa agcagttaca gaatataatg
    15541 aacgcactct aaatagaatg actattgctg atttactcaa ccgaaaggcc aaaaagggcg
    15601 acagcgttct gttagatgat tgtgggatta ttcctttttt ttctcgagaa gacattcgat
    15661 tcatcgacgc gcaatgtttg aacaatagcg atttaaccca aaaaccattt aaaaacgatt
    15721 taagtcttta cgctgattat ctcaattctg aagtgaagcc tgattgggtg atcaccaatt
    15781 actaccctat ggaatcacga ggtgattatt taacagattt gctatatgaa aagcattttt
    15841 ttaaaaatta tcatttaatt agtacattaa gctcgggttt ttatttaaac aaaaatcaac
    15901 cacaagggaa aaaaatgatt gattttgtgt accaaatttt taagcgtgtt aataaacctg
    15961 gttacaatgg ctccctggaa ttggaatccc aggagccaaa gtgatgacta ttgttgctca
    16021 gagggtgagc tttggtttga aaaatctgcg gtcggttggg cggcttttaa attttgcaat
    16081 agcataatgc tgttagttaa taatatccgt tcttgtattt gtctgtccaa atacatttga
    16141 taattaattt cagccagcaa taccgctatt tctttttgaa cggtggcagg ggatgcgtca
    16201 ttaatttgtt taatccattg ttggtttggt tgtccgggag tgttggaggc ggttggatca
    16261 aataatcgtc gagttgccat gttaaattca ttcaacgcct gacttgtgat attggaacta
    16321 ttttggtctc cactcatatt ttggggtaat cttttggata aaatataata gagattactc
    16381 acgcctacag aagtttgtgc ggcgtaaaca cgcaaattat taaaataacc gctcaatgtt
    16441 gccgccgctt gtttttgctg gacttcatta taaccttggg aacctggttc agccttagcc
    16501 tgattccata actcagtgta ggcgctcaac ttgggcaagg aaggaggagt gacttgagcg
    16561 ctggcgtatc taataaaatt aagcgcttgc tgggcttggc tttgtgcctt taatccggat
    16621 ttttgctctg attgtgccgc agaggtatcc aggagtaatg gcgcaatcaa agagttactg
    16681 ttcagttggc ttatgagcgg tttattgtac gtgtagctat aaaaaggttc cagcgagctt
    16741 tgattaggca aggctggagg taaagtgcca attatatttt gcatgaccag attttgatac
    16801 atcaggttgc aatttggcaa ccagtttttt tgctcattgt ccatacaaaa actgtaatcc
    16861 ggggttccca gaatattgaa tactgcctga ctgacgggat catttaaata agtcccggag
    16921 gttgggattt gtatggcaga aaaaaccccg gaaggagtgg gggcttgagc actggttgtt
    16981 gattgatcta ttaaactatt agcggtaatt tttccatctg acccggatga tggggagctg
    17041 aagttttgat atttaaaggt gctgtttgcc aagttattta ttaatgcatt tccttgcacc
    17101 ttattggtga cgaaattcat taagctctgt gacatggcat cgacaggaat agcgcccaaa
    17161 aaggtattgt aagcgtaatt ttgaatgagt tgggctacat tggaattaaa tagctgtgaa
    17221 taaggcggag ttggtggttt gcccttttca gtaatatcat agcctaaata tttccccaga
    17281 ttcgtcaggt aagttaccag gctattcgtg tttttgcttg tctgttgatc atacgtgtca
    17341 taaccctgtt gagcaaaaac agataaggtg gtaaaacaaa acagattggc caaggccagt
    17401 ttaggtaata ctttcattat tcatcccctt cgagtgctcg taaaacgagt tttagacggt
    17461 attggcaaaa aactcgcgat aataacctta gtctttcaaa tacaatattg cgttttagaa
    17521 aaaaacctgc cgggtcattg ctgcttaaac tggtttcttc cgcatggatt aaaacacggg
    17581 aggcgctacc aggatggact gtatctatcg cttgaagcaa gcgtaagcct cttcctgatt
    17641 tgatgtttcc aacgatacga ataaatttgt cttcttctgc tagcaagccc aagtcaattt
    17701 tttcaatatc atcaagttct tgccctaact gtttcattgc atcttccagc tccggatttc
    17761 catcaagagt ccagttttcc acactttcca taaaagtgat gaccctgtaa atcatggggt
    17821 caaggtactc aaaccagtac tttgcggagg cttcatggct taaatcaggc attatttact
    17881 ctttttttac tgagatacgt tgatagacga aacaactact taatactata gtatattatg
    17941 atatataaaa gtccattact taaaattgtg aaaaagtaaa aaatatgaaa aagaaatcgg
    18001 gatcaaggat cgtaagagtt attacaagga ttatcaatgt tcgtaaatgg gtcgattggg
    18061 acagaatgaa gtcattcacc ttatatttgg tcaatggcgg taagcgttta ttcgtcccac
    18121 aggaaccaac tcatgtcgaa tcatttgatg aggcagccaa gaaactaaaa ttgagcgagg
    18181 ctgacttgat aatcaaacaa aaagccttgt tccggttaag tgtgattatg gttatcgctg
    18241 cgtttatgct actcatttac acgggttatc aactgtttta tggttcatgg aaagctacaa
    18301 taataagctt ggttgtggtt atgatcgcat tggtactagc ctttcgttat catttttggt
    18361 actaccagat taaacaacgt aaattgggat gtaccgttaa agaatggtac aggcaagggt
    18421 tattgggtga gaaagaatga ataaattagc tattacggtc ctcctttgct tttttcctgc
    18481 gcttgcactg gctgaaagtg gcgccttaag tttcgcgcct ccggcaagcg atttgtccgt
    18541 tgttttttta ggtaacctgt ttggtgtagt cgatggggtc ttgcacggaa cgggaagcca
    18601 gataatgggc aacatgtttg gtgtcttcaa ctccgctgtc cttgctttgg gtggaatcat
    18661 cattatgtat accctcatgg tttccaccat gaacaccgct catgaagggc aaatgcttgg
    18721 ccaaaaatgg tcctctatct ggattcctct tcgttccact ttcggtttgg ctttgttaat
    18781 acctaaagca tccggttatt gtatgatgca ggtatttttt atgtgggtta ttgtgcaagg
    18841 ggttggtgca gccgataaaa tatgggaagc tgctttgagt tatctcaatc ggggtggtgt
    18901 gatcatacag gctcagtctg acccaaccaa gagcctcaca gataagggtg ggagttctac
    18961 aggcatagca aatggggcat tgaccatatt ggctggtcaa gtctgcatgt tgggtttaca
    19021 aaaacaatta caagcgcaaa gggatatgta tttaagtcaa agtaagagcc ctccttgtgg
    19081 cggtaatcct agtactgaaa tggataagtt ttgccgtaca gcgattccgg acttcatcag
    19141 tacagtgaat tttgtcaaga agcaaaacga tagcaagcct gaaggaccat ttagcattga
    19201 tatgccaaac ttcgagcaag gctcaacatt tgatttttta aatggtattt gtggcacagt
    19261 aaaatggaag agtatttccg gtttgagcgc taatatggga aaaggcataa atcttaattc
    19321 ttcggaattg aaaacagccc aattgtctcg cgcgattgct atacagcaaa tgtacgtgac
    19381 tttatcaacg gtagcccagg tgatggttaa taatgatccg gctttttcaa caacctcgtc
    19441 taccggaaat tcaaaaaatg atttttccga tattgccaaa caacagtttg gggtgcctta
    19501 taaagcatct ggtcagccct gcgagatata tagtgatgtt tgccaaacat ggggggcagc
    19561 aaccagttcc tcaggatcta ctccgggggt attgtttaat ggcacggaat ttttaggggc
    19621 gatcaatgac tacaatggca ttatgacgcc gactgttaat ctattaaagc aaaaagatgc
    19681 tttagcagac gatgacacat cccgtaagtt tattgcagaa gccaatgcca aagggtggat
    19741 catggccgga tcttatttct ttgatttggt gaaactcaat ggaagcgcag taaaagacga
    19801 tgctgatttt gatagtgtga cagggcttga ggatagtaat tttgatctgg aacaactcac
    19861 caagccattt ggtaaaacat gtcagggcac tcgtgcttta ttgtgcacct ggtttcaaaa
    19921 caagagtgat aagctggttc aaatccagtc cctaatcaat ggcgttcctg ccttgagtca
    19981 agatggggtc aagcaacctg atctaaagcc taatactgga cgtaaagctg tggaaggatt
    20041 attgtcttca acagtttatg gctttgtcaa caactccatg atggtccagt tgcctggcca
    20101 gccgggaata aaaccgttga cctttgccaa tcttattaat tttaaagtag atacctcgct
    20161 ttattacatg aagcaccagg attttgattg cggcagggtg aagattttgt tcttttcttt
    20221 ttgccttggc cgcatgatgg gtgatttatt ttataactat gttttcaggt atgtgtacaa
    20281 cttctttcta gccatattcg gggaaatgat taattccata gtcatggcct ttttaatgat
    20341 tcctttacaa ggtatgaaag atattttcat agtgggcgtt caaactctta cgaagcctgg
    20401 agtaaacccc attgtcgcat tggctaatat gggaacgatg tatattaatt tttctggtac
    20461 tttgtggcta acgctgttga atatggcggt ggtcagttca ctaatcccac tttttggtat
    20521 ctttatcttt gccttgatta tgatggctat gcccttgttg atggcatgga taggaaccat
    20581 ggtatccata ggatttgtta cggcatatta tatacccgtt ttaccttata tgatttttac
    20641 ttttggatca tttgcctggc tgatagccgt tatcgaggct atggttgctg cgccaattgt
    20701 cgcattgggt gttacgcatc cggaagggaa tgaggctttt ggtaaaggcg aatttgctat
    20761 tatgattttg gttaatgtct ttttaagacc ttctctgatg ataataggat atattacagc
    20821 aattgccttg tcctatgttg gcgtctggat tttgaatgcg ggatttgatc acgccatttc
    20881 tttcattcaa tcagatcaag gtatcgaaac atgggaaaag ggtgattttt caaaaacaca
    20941 tgctgattca gcatactgta aaaggtatcc tgctgactgt aaaatagcag aggcagggca
    21001 gacagtctgg aattggtccg ggacaaaggg agatacgtcg caatggaatg attttggatc
    21061 caaattgagc ggtgattatc aatcgacggc attacaatct gatacattaa atcatggtta
    21121 tacgggttgg gctggagtgt atgctttctt cttctccatc ttaatttata ccaccatgta
    21181 tttaattatc gtgcaaaaag ccttcacgct gattgctcac ttgccagata aagtgctgag
    21241 atggataggc ggtagccctg aatcttttgg tcaagaaacc atgcaatggg gtgaagaggc
    21301 caagggtaaa gtagagaaag caggcgaagc cacttatgga gctgagaaag ctattggcga
    21361 caagttggga gctaaagggc aagaaatgct tggcaaattt ggaccccaag ctaaacagat
    21421 ttcagaaaat gcgggtgatg tttcagggaa aggtaaaaac agcggcagcc agactgggcc
    21481 aagctccaaa ccggacacag gtcaactggg tggttcagac agtggtaccc cgaccagtac
    21541 accacctccc gaataacatt ctagtacaat actataagaa gggctcaaag gagcctttct
    21601 tattatttat gggcagttca ttgtattaag gttttgacag ttagttatta atgctatttg
    21661 gacggccttt taaaacgagg attactatgt tagtgactaa ggaagtggaa tacgaggatg
    21721 gtgatactgt gtgcaagggt tttattgcct atcattccag tgtctcaaaa ccgttacctt
    21781 gcgttat
//
LOCUS       DATNSN010000037        21295 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.037, whole genome shotgun sequence.
ACCESSION   DATNSN010000037 DATNSN010000000
VERSION     DATNSN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.037"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..466
                     /locus_tag="VAH76_001186"
     CDS             <1..466
                     /locus_tag="VAH76_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385830.1"
                     /translation="IDEQYFPVKTIQKIYNECYDLEYTATVMVPSALNGQPDSVYYPL
                     QCPFAKINTFKTNQSNSAYSELEMLKNVLLAYQSEFTADNSSAFGSLLYHVSKQTEFS
                     FYHYKSSSNKSINSSEELGGTDNRFAFSYCSDRTQFASDAKLFRGCVRLSKQ"
     gene            477..1034
                     /locus_tag="VAH76_001187"
     CDS             477..1034
                     /locus_tag="VAH76_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1385831.1"
                     /translation="MRILETERLFLRTFQENDVESLIAISQDEKVMQFFPAIPTREEI
                     IAFIDRIITHQEEKNFSLYAAEIKNTGEMIGFVGLFTATFKAHFTPAIEIGWRLSSKH
                     WNQGYATEAAKAVLDYAFRKLDLDEVVSFTSELNKPSIRVMQKIGLHTNSEDNFDNPK
                     VPSGSPLMGHVLYRLKRSEYLSTQY"
     gene            complement(1020..1796)
                     /locus_tag="VAH76_001188"
     CDS             complement(1020..1796)
                     /locus_tag="VAH76_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HEO1385832.1"
                     /translation="MHKLIVVGSGIKSISHLTEETKRVIQSADKVLYLINEDNLKHWI
                     QREAKNSESLDSIYFSSEKRVEAYQALTTHIIEEYKKVSILCVVFYGHPTVFADSALN
                     AVRQIKQDNGEAIILPAISSQDCLFSDLEIDPGDQGCFSIEATELVLFERCIDIHAHL
                     ILWQVANFGRTDGKKTNNLSILKDYLSGYYPADYSICLYEAPSLPTCPSRIEWIQLSN
                     LELSAISSITTVYIPPIEKKAISNKYLKLLNLKIDDLILS"
     gene            complement(1789..1974)
                     /locus_tag="VAH76_001189"
     CDS             complement(1789..1974)
                     /locus_tag="VAH76_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385833.1"
                     /translation="MSLMEELEAAVGNEYLISHKNLLLNTNQNTQRQAYSNKIVSIQK
                     QYQPDSNKIVNIKRVNA"
     gene            2357..3484
                     /locus_tag="VAH76_001190"
     CDS             2357..3484
                     /locus_tag="VAH76_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO1385834.1"
                     /translation="MGRKKSPGLFKRGDYWHIDKAVFGCRIRESTGTGNLEEAEKYFT
                     KRIEEIRQASVFGVRPKRTFMEAAIKFLDENQHKRSIDSDAGRLRVLVQYIGKMPLDS
                     IHMGTLQPFIDGRRQDQVKTRTINHGLKIVRRICNLAATEWIDEFGLTWLLNAPKIKL
                     LPEDDLRKPYPLSWDEQHRLFAELPVHLEQMALFAVNTGCRESEVCHLQWDWEIKLPQ
                     LPHLLVFIIPPEMVKNGEERLVVCNQTAKSVVDSQRGKHKQFVFTFKGNPITRINNTG
                     WKEARKKAGLEFVRVHDLKHTFGRRLRSVGVSFEDRQDLLGHRSGRITTHYSSAELQN
                     LYEAGNKVCEKQQSGVVLTLLRNPNNRKEKDTSHGRKVVSL"
     gene            complement(3577..3653)
                     /locus_tag="VAH76_001191"
     tRNA            complement(3577..3653)
                     /locus_tag="VAH76_001191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3617..3619),aa:Ile,seq:cat)
     gene            complement(3743..5608)
                     /gene="rpoD"
                     /locus_tag="VAH76_001192"
     CDS             complement(3743..5608)
                     /gene="rpoD"
                     /locus_tag="VAH76_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO1385835.1"
                     /translation="MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHF
                     DVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVR
                     MYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
                     LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPE
                     QAKVYFDELREAFEKAMKALKEHGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHF
                     RQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETIISKHAKKLDLQR
                     IEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQILQETGREATPEELAEKMELSEDKIRKVLKIAKE
                     PISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAKVLRM
                     RFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG"
     gene            complement(5695..7428)
                     /locus_tag="VAH76_001193"
     CDS             complement(5695..7428)
                     /locus_tag="VAH76_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO1385836.1"
                     /translation="MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHN
                     EKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGT
                     GEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
                     HNLEKAFPNNQRELLSTGMLIKGDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKD
                     QKPKYLNSPETVLFQKSRELYGLHQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVA
                     TLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLP
                     NEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYL
                     QKMVEGSYKQLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLL
                     QNPEIYSIAIQQINPALLHEQEHHILLKLLQQLKDKPNANTATLIEFWRNSSYFELIV
                     KLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERLSLQNLL
                     KERHGQAEIEK"
     gene            complement(7437..7880)
                     /locus_tag="VAH76_001194"
     CDS             complement(7437..7880)
                     /locus_tag="VAH76_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2359 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385837.1"
                     /translation="MTIKEQINNDIKDAMRAKDKNLLNALRLISAAVKQIEVDERIEV
                     DDERMLVILDKMSKQRKESIAQFEKASRDDLVAQEQFELNVISKYLPEPLTDNEIEEL
                     IKDAIKSTGAEKMSDMGKVMAILKPKLQGRADMAQVSARIKAKLS"
     gene            complement(8083..8322)
                     /gene="rpsU"
                     /locus_tag="VAH76_001195"
     CDS             complement(8083..8322)
                     /gene="rpsU"
                     /locus_tag="VAH76_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO1385838.1"
                     /translation="MPTVRVKEGENPEYALRRFKRSCEKAGILTELRRREFYEKPTAE
                     RKRKQAAAVKRHLKKISRDVSSRRGVGHRRKKSTT"
     gene            8527..9528
                     /gene="tsaD"
                     /locus_tag="VAH76_001196"
     CDS             8527..9528
                     /gene="tsaD"
                     /locus_tag="VAH76_001196"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO1385839.1"
                     /translation="MLVLGIESSCDETGVAVYDSESGLLFHALHSQIATHRVHGGVVP
                     ELASRDHVNYLVPLVDEVLTKAQIRKNQLDGIAYTAGPGLIGALLVGSCFAKSLAYAL
                     NIPALAIHHLEAHLLAAKMETPSLDFPFIALLVSGGHCQLIEVNNIGEYRLLGDTLDD
                     AVGEAFDKTAKLMGIPYPGGAVLANLADQCLSTPYQFPRPMTDRPGLDFSFSGLKTHA
                     LNTWNQSEKKESDRSEIAKAFQQAVVETLIIKCKRAIKESQSKRLVVAGGVGANKALR
                     SALQKWIKDIKGEVYFPALEYCTDNGAMVAYAGCLRMMRGESDGGLEVMVKPRWPLA"
     gene            complement(9951..10784)
                     /gene="plsY"
                     /locus_tag="VAH76_001197"
     CDS             complement(9951..10784)
                     /gene="plsY"
                     /locus_tag="VAH76_001197"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO1385840.1"
                     /translation="MALFIFLILVGYLMGSINSAIIVCRTFGLPDPREEGSKNPGATN
                     VLRLGGKQYGIMVMVFDALKGILPVILAKFLSAEPVTVAFTALAAVVGHMYPVFFHFR
                     GGKGVATTIGALLAFHFVIGVMVAATWLLVANFWRYSSLASIASISLAPFYSLILVGN
                     LNIFPPLFMITILVLYKHRDNFNRLIDGKEPKIKFKHSVIEEIMEASPATSAEQEFPG
                     KEVIDTNIDETEKTEQAEAVKKPKAKKATTKAKKTASKEETTKKPKSTKPKTKTVKEK
                     E"
     gene            11138..12547
                     /locus_tag="VAH76_001198"
     CDS             11138..12547
                     /locus_tag="VAH76_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO1385841.1"
                     /translation="MDAIYLLSATEIIRRIKMKELSAAEIMVAHLNHIDKINPVINAL
                     TERIPSEECLKQAKEIDKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGE
                     KAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
                     SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNL
                     ISFGPMARSVSDLRLGLSVLAGSDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVD
                     AEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGV
                     NNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAK
                     PHGIGIKEISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAE
                     RVEQLMGGYKPPSLIHSLQ"
     gene            complement(12579..13706)
                     /gene="hemW"
                     /locus_tag="VAH76_001199"
     CDS             complement(12579..13706)
                     /gene="hemW"
                     /locus_tag="VAH76_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEO1385842.1"
                     /translation="MLPLSLYIHIPWCIRKCPYCDFNSHKSPDVLPEHQYIQALIEDL
                     KTDISSYNTREINSIFIGGGTPSLFSAEAYNNLFNELKRILPFAKNIEITMEANPGTV
                     EQHRFTDYRQSGINRLSLGIQSFNPNHLKILGRIHDEKQAHSAIDTARKAGFDNLNLD
                     IMHSLPNQSVTQGLQDLKNALSYQPEHLSWYQLTIEPNTVFYKHTPPLPSEEEDYLLE
                     EQGFALLHDSGYHRYEISAFSKPEKQARHNINYWLFGDYLGIGAGAHGKMTTPNAIIR
                     TRKHRQPKDYLDNEKPYLAARETITGKELLFEFMLNTTRLEQSIPLELFTTATGLELN
                     QLLPLLKLANKKQLINLTDTHWQVTPLGRQYTNDLQALFLP"
     gene            13788..14348
                     /gene="nudE"
                     /locus_tag="VAH76_001200"
     CDS             13788..14348
                     /gene="nudE"
                     /locus_tag="VAH76_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEO1385843.1"
                     /translation="MRNKPKCKQRTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGH
                     GAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAA
                     RELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAHELLEKP
                     DFTEARSIAALFLVKEWLNKRKSYDK"
     gene            14338..15330
                     /locus_tag="VAH76_001201"
     CDS             14338..15330
                     /locus_tag="VAH76_001201"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEO1385844.1"
                     /translation="MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLEL
                     EPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDL
                     LQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
                     ELRARTQLAKKADVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKE
                     TFVSTVQQRGAAVIKARGSSSAASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGV
                     DEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSERDTVKSLGLLD"
     gene            complement(15428..16348)
                     /gene="mavF"
                     /locus_tag="VAH76_001202"
     CDS             complement(15428..16348)
                     /gene="mavF"
                     /locus_tag="VAH76_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavF"
                     /protein_id="HEO1385845.1"
                     /translation="MQNIWLLCFYKLLLKKGLWRNIGMEDPFSVLLKSLQSVFHLEAM
                     RNGKYTFPAVQHLKEATENYNPKARNTFIELLRAIREALPYIEKWRINFNVIRKSMDA
                     LAKLHHMPTIDWNQVLSHPKVSPRFQFSALNHSHHDYDLMTWLEKKIGKPFSQLDHTE
                     LTQTLVDNRDRLGFSQKLSNHLKKDPDFLYNIILRSERNFIKISQTRLILYLTDEQLA
                     RAIIKHIPVLMHKRKEPFEQIEQLIHTLNEILSNGRSVSTLLRNTDAKTILENSTLFQ
                     MYLSEEYKSRHQHPNKDPDLQTKESLKPGL"
     gene            16479..17018
                     /locus_tag="VAH76_001203"
     CDS             16479..17018
                     /locus_tag="VAH76_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO1385846.1"
                     /translation="MITVGNKFPEFQLKATVANDINNAFQMISNETYRGKWLVVFFWP
                     KDFTFVCPTEIAEFGRLNSEFADRDAQILGGSTDSEFVHLAWRNQHPDLSNLPFPMLA
                     DVKRELTANLGILDDEEGVAQRATFIVDPQGVTRFVMVTDLNVGRNPAEVLRVLDALQ
                     TDELCPCNWKKGEETIHIK"
     gene            17041..17556
                     /locus_tag="VAH76_001204"
     CDS             17041..17556
                     /locus_tag="VAH76_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO1385847.1"
                     /translation="MLENVKESIPEVAKDIRLNLTKVLDLEQSDGLSEHQILGSALAV
                     SYSLGNEVLIKGLKELVHNEQIVRAAQLAASLMAMTNIYYRFVHLTEIKEIEHIPAGL
                     RMQGMMTPGVDRVTFEILSLAVSALNGCGACVSSHARQLKEHGLSDTSLARVGRITAV
                     IHAVHVSARIK"
     gene            complement(17600..18088)
                     /gene="sodC"
                     /locus_tag="VAH76_001205"
     CDS             complement(17600..18088)
                     /gene="sodC"
                     /locus_tag="VAH76_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodC"
                     /protein_id="HEO1385848.1"
                     /translation="MNKSGIILIGTILFSSMAIADDLTAPIYTTGPKPVAIGKVTFTQ
                     TPYGVLITPDLTNLPEGPHGFHLHKNADCGNHGMHAEGHYDPQNTNSHQGPYGNGHLG
                     DLPVLYVTSNGKAMIPTLAPRLKLNDMHNLAVMIHANGDTYSDNPPQGGGGDRIACGV
                     IK"
     gene            18393..20417
                     /locus_tag="VAH76_001206"
     CDS             18393..20417
                     /locus_tag="VAH76_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEO1385849.1"
                     /translation="MELRIDNTPFKAIFQPLDLGFTQIKNRLLMGSMHTGLEEDKENL
                     NRLASFYRERAQGGVGLIVTGGFAPNRAGRLAPFAAKLTNSKEQHRHEVITQAVHEAG
                     GKIALQILHAGRYGYHPFIVAPSGIKSPISPFKPWEMSKRRIIKTIKHFARCARLAQL
                     AGYDGVEIMGSEGYLINQFIVTHTNHRRDEWGGSFSNRIRFPIEIVRSVREAVGEHFI
                     IIYRLSMLDLVAEGSNWQEVVTLAKAIEQAGASLINTGIGWHEARIPTIGTMVPSAAF
                     TQVTQHLKPEIKIPVITSNRINTPELANQLLEDGVADMISMARPFLADPCFAEKAKLG
                     ESKAINVCIACNQACLDQVFVNKTASCLVNPRACNETTLVYETTNQPKAIAVVGSGPA
                     GLAFAVTAAERGHKITLFEKANQIGGQFNLAKKIPGKEEFQHTLNYFAYQLQKYKVDV
                     RLNTEATVELLKDYDEVVLASGIIPRVPEIKGIDHKKVVSYIDVITQNKPVGSRVAVI
                     GAGGIGFDVAEFLTHDHQLTSEQFYNEWGIDISGQHRGGLKPPEIAPSPREVYLLQRK
                     KEKMGKRLGKTTGWIHRSSLKHKQVKMFSGVNYERIDDQGLHLTIDDKPQVLEVDSIV
                     ICAGQIELKNLYEPLKMAGKLVHLIGGAYKALELDARHAIDQACRLAALV"
     gene            20414..>21295
                     /locus_tag="VAH76_001207"
     CDS             20414..>21295
                     /locus_tag="VAH76_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO1385850.1"
                     /translation="MSKIAVVINPIAGGGRGKKIWKVLKPGLHALFEQTVYRISNRVN
                     DLEAITDGLLAENPDSLLIIGGDGTLNHVLNGLIENDKLKNPQIKIAFFNAGCGGDFT
                     RQFPQQKMTEFLDRLSHNQFIKCDIGKITLANQVSRYFINIASCGLSGHVVLRVSKSK
                     WLKKLGGTLNYLLHALTGLMTYRKTKVRVQIDDALPFDSSILLMAICSGQYFGGGMHV
                     APMAKVNDGLFDVVSFNEFSKLNALLKLYKIYSGGHLLDNNVHYVQTKKIKIEPLEES
                     KIEIEADGEIIGCLPAQF"
BASE COUNT         6136 a         3920 c         4579 g         6660 t
ORIGIN      
        1 aattgatgaa caatactttc cagttaaaac aattcagaaa atttataatg aatgttacga
       61 cttagaatac acagcgacag taatggtccc gtccgctttg aatggccagc ctgatagtgt
      121 ttactatccc ttacagtgtc cttttgctaa gattaataca tttaaaacca atcaaagtaa
      181 tagtgcttac agtgaacttg aaatgctaaa aaatgtgtta ttggcttatc aatcggagtt
      241 tactgcagat aacagttctg cttttggaag tctgctttat catgtaagca aacaaacgga
      301 attttctttt taccattata aatcttctag taataagagt attaatagct ctgaagaatt
      361 aggaggtact gataacagat ttgctttttc ctattgtagc gatagaacgc aatttgccag
      421 tgatgcgaag ttatttagag gttgcgttag attaagcaaa caataaggct atatagatga
      481 gaatattaga aacagaacga ttatttctgc ggacttttca agaaaatgat gtggaatcat
      541 taattgccat tagccaggac gaaaaagtca tgcagttttt tcctgccata cctactcgag
      601 aagaaatcat tgcattcata gatagaataa ttacacatca agaagaaaaa aatttctccc
      661 tatatgctgc tgaaattaaa aatactggtg agatgatagg ttttgtaggt ttgtttacag
      721 caacatttaa agctcatttt actcctgcta ttgaaattgg ttggcgttta tcttcaaaac
      781 actggaacca aggttatgca acagaggccg ccaaagcagt ccttgattat gcttttagga
      841 aactagattt ggatgaagtt gtctcgttta catccgaatt aaataagccg tctattcgag
      901 taatgcaaaa gatcggatta cataccaatt cagaagataa ttttgataac cctaaagtac
      961 cgtcaggtag ccctttaatg gggcatgtgt tgtatagatt aaaacgatca gagtatttat
     1021 caactcagta ttaaatcatc aattttcaga ttgagtaatt tcaggtattt gttgctaatg
     1081 gccttttttt cgatcggtgg aatatatact gttgttattg aagaaatagc tgatagttcg
     1141 agattactaa gttgtatcca ttctattcgg gaagggcaag taggaagaga gggtgcctca
     1201 taaagacaaa tggagtaatc tgctggatag tatccactta gataatcttt taaaattgat
     1261 aggttgttag ttttttttcc atcagttcta ccaaaatttg ctacctgcca aagtattaaa
     1321 tgcgcatgaa tatcaatgca ccgttcaaat aataccagct ctgtggcttc aattgaaaaa
     1381 catccttgat cgcctggatc aatttctaga tcactaaaca agcagtcttg ggaagatatt
     1441 gcaggtaaaa taattgcttc gccattatcc tgtttaattt gtctcacggc attaagtgca
     1501 gaatctgcaa atacggtagg atggccgtaa aaaacaacgc ataaaattga aaccttttta
     1561 tattcttcaa tgatatgagt tgttagtgcc tgatacgctt caacccgttt ttcgctacta
     1621 aaatagatag aatccaacga ttcagaattt ttcgcttctc gttgaatcca gtgtttaagg
     1681 ttatcttcat taatgagata gagtacctta tcagcacttt gaataacccg tttagtttct
     1741 tcggtcagat gagaaatcga ttttattcct gaccctacta ctattagttt atgcattaac
     1801 tcgctttata ttaactatct tattgctatc aggctgatac tgtttttgta ttgagactat
     1861 tttattagaa taagcctgcc tctgtgtatt ttgattagtg tttaacaata aatttttgtg
     1921 gcttatcaaa tactcatttc ctacagcggc ttcaagttct tccattaaac tcatttgatg
     1981 taccttttat taaactttga tagccattgt acaaataaaa ttcttatagg tatagcctaa
     2041 aaaaacaatt gcagtgattt actcattatg ctctaataac tactcgtatc gagtagttat
     2101 gatttgagaa ttacaggagt tgagaagtca tgagcaatat tcatctaatc ccccgtttga
     2161 ttcgcttaag agatgctcct acctatgtag gtatggatcg acacagattt aataaagacg
     2221 ttagaccaaa tttggtagaa attccgatgg gaattcaggg aattgcattt gatcgacttg
     2281 atttagacgc atgggtcgac gattatatac agtgtagtgg tcgtcctgcg gtaaccaagc
     2341 ataggagttt ggaacaatgg gacgaaaaaa atcgccgggt ctcttcaaaa gaggtgacta
     2401 ttggcacatc gataaagcgg tctttggatg cagaattcga gaaagcactg gtacgggcaa
     2461 cctcgaagaa gcagaaaaat actttacaaa acgtatagaa gaaataaggc aagcgagtgt
     2521 ttttggagta agacccaaac gcacgtttat ggaagcagcc attaaatttt tggatgaaaa
     2581 ccaacataag cgaagcattg attctgatgc agggcgttta cgtgttctgg ttcagtatat
     2641 tggtaaaatg cctttagact cgattcatat gggaacttta cagccattta ttgatgggcg
     2701 acgtcaggat caggttaaaa caagaacgat taaccatggt ctaaaaatag ttcgtcgaat
     2761 ttgtaatttg gctgcaaccg agtggattga tgagtttggg ctgacttggt tgctgaatgc
     2821 ccccaaaatt aaattgctgc cagaagatga tttacgcaaa ccttatccac taagttggga
     2881 tgagcaacat agactctttg cagagttgcc tgtgcattta gagcaaatgg cgctatttgc
     2941 agtgaatact ggatgtagag aaagtgaagt atgtcacttg caatgggatt gggaaattaa
     3001 gcttcctcaa ttgccgcatc tgttagtgtt catcattccc cctgaaatgg ttaaaaatgg
     3061 tgaagagcgc ttggtagtct gtaatcaaac ggcaaaatcg gtagttgata gccaaagagg
     3121 aaaacataaa cagtttgtgt ttacctttaa aggcaatccg attacccgaa taaataatac
     3181 aggttggaaa gaagctcgta agaaagctgg tttggagttt gtgcgtgttc atgatctgaa
     3241 acataccttt ggtagacgat taagatctgt tggagtgagt tttgaagaca ggcaagatct
     3301 gcttgggcat cgttcaggtc gtattacaac gcattactca tcagctgaat tgcagaatct
     3361 atatgaagct ggaaacaagg tttgcgaaaa gcaacaaagt ggggtggttc tcacgttact
     3421 tcgtaatcca aataatagaa aagaaaaaga tacatctcac ggacgtaagg tagttagttt
     3481 atgagtttgt gtctactgtg ccccacaaaa tccccacaat ggtttttgag gggttgggat
     3541 aatcgattgc aagttgttga ttttactgac tttaattggt gggcccacta ggacttgaac
     3601 ctaggaccaa cggattatga gtccgctgct ctgaccaact gagctatggg cccggagagg
     3661 gggtcatttt aaacttaatt tctttaatgt tctactattt tttatatttt tttaatgact
     3721 gtttatataa acagtcatta aattaacctt catccccttc aaggaagctt cttagttttt
     3781 ctgaacgtga agggtggcgc aatttgcgta gtgcttttgc ttcgatttgg cgaatacgct
     3841 cgcgggttac gtcaaattgt ttaccaactt cttccagggt atggtctgta ttcatttcga
     3901 taccaaaacg catacgcaga accttggctt ctcgtggggt cagagtttcc agtatttcta
     3961 aagtggcttc tcttaaacct tccgctgtag ccatatcgat tggcgattca atgttgttgt
     4021 cttcaataaa atcacccaga tgagagtcgt catcatcacc gacaggggtt tccatggaaa
     4081 taggttcttt ggctatcttt agaactttgc gaattttatc ttcacttaat tccatttttt
     4141 ccgccaattc ctccggtgtc gcttcccggc cagtttcttg caaaatttgg cgagaaatac
     4201 gattgagctt attgatggtt tcaatcatat gaaccggaat acgaatagtc cgcgcctgat
     4261 cggctatgga tcgtgtaatc gcttgtctaa tccaccaggt cgcataggta gaaaacttat
     4321 agccacggcg gtattcgaac ttatcgactg ctttcatcaa gccaatgttg ccttcctgga
     4381 tcaaatcaag aaactgcaag ccgcggttgg tgtatttttt ggcgatggaa atcacgagac
     4441 gcaagttagc ctcgaccatt tcttttttag cacgtcttgc tttggcttcg ccaatagaca
     4501 tcttgcggtt aatgtctttg atttcactaa tggttaaacc atactcgatt tcaaaagagg
     4561 ccagcttgga ttgtaagcgt agtatttctt cccgattttc ttctatacgc tgaagatcaa
     4621 gttttttggc atgtttgctg ataatggttt ctaaccagct tagatctgtt tcctgacctg
     4681 ggaaggtatc tataaacaat tttctgggga tgcgtgcttt ttcgatgcaa agacgcataa
     4741 tgcttctctc aaattcacgg atatgatttc gcaattggcg aaaatgacga gttaatttat
     4801 caacttgtct ggaagtcaat ttgagtttca gaaaagattc tgacatggaa tcgagcagct
     4861 cgactgtttt tttattcgta cggccatgtt ctttgagggc tttcattgcc ttttcaaagg
     4921 cttctcgcag ttcatcaaag taaacttttg cttgttcagg attgggtcca tcatccacat
     4981 cagatatgcc gccttcgccg tcttcatctt cttcatccac atcaagaagt atttcttcct
     5041 gctgctcttc atccagcatt gaaccaatgt tagtggcagg tgctatttct tcatcaacat
     5101 cagcaaaacc actaatgatt tcattaaggc gaatttcttc tgcaaggaag cgatcatagt
     5161 cttcaagaac cagttgcact gtttccggat aatgcgccag ggattttaga acctggtaaa
     5221 ttccttcttc aatgcgtttg gcaatgcgga tttcaccttc acgagtcagc agttctactg
     5281 tgcccatttc gcgcatatac atacgaaccg ggtcagtggt tctgcctgtt tctttatcca
     5341 cagaggcgag taccatggct gcttcttcaa tatctgggac ttcctcagta tttaataaga
     5401 gcgcaagttc atcttcactg ggtggaagct caaaaacttt gatgttcatg ccttccagca
     5461 tgctaatgat tacatcaaaa tgctctgtat ccacaatatt aggtaataag tcattaattt
     5521 gactgtaagt caaataacct tgctctttac ccaggctaat gactttcgta atttgtgagc
     5581 tttgctgatc ttggtcattc atggtcatag gcacttctgt tggaacaatg ggaaattaca
     5641 aaaagtttta aattataaac tgggtagctg caacatacca gtatttcagt atttttattt
     5701 ttcaatttca gcttgcccat gtctttcctt taataaattt tgtaaactga gccgttcagc
     5761 ttctgttaag ccagtttttc tggatttttc tatgtattgt cgtatcaata tctcacgatt
     5821 ttgtttttgt aaaaataata agacatcaat aaattcttta gtcagctcct gctcaggcac
     5881 ttgatgatcc caggccgcca atttaactat caattcaaaa taactgctat tgcgccagaa
     5941 ttcaatgagg gtggcagtat tggcattggg cttgtctttt aattgttgca ataatttcaa
     6001 taaaatatga tgctcctgtt catggagcag agcaggattg atttgttgta ttgctataga
     6061 ataaatttcg gggttttgta agaggagcgc gatagcaata cgcagaggtg aacgagaaat
     6121 agcagcttgt acttccggct tgctttccga tttatcagta atcaaattag ttaaccgatg
     6181 gctttcaata tgggttaagc gtgccaggtc atcaataagc aactgtttgt atgagccctc
     6241 aaccattttt tgcaaatagg gtttcactgc gttgataagc tgtgtttttc ctgcagggtt
     6301 tgacaaatta atgtctttac ttaaagtatc aaagaaaaag cggtggagtg gtgttgcttg
     6361 ttgcaagagc tcaaggaaac cgttttttcc ttcatttctt accaggctat ccgggtcatg
     6421 ttcattcggc aagaaaataa atccggcatc taatccggca ttcaaatgcg gtaaactgct
     6481 ctccaatgct cgccaggctg cttgtttacc tgccgcatcg ccatcaaagc aaaaaatgag
     6541 atgtttggta tgctttgcca atagttgtat atggaatgtg ctggtggcag ttcccaacgt
     6601 tgccaccaca ttaaaaatgc catgttgtgc caaggcaatg acatccatgt aaccttcaac
     6661 aacaataata ctgtcgggat ttttctgctg agacaggatt tgatgtaagc catataactc
     6721 acggcttttt tgaaaaagta ctgtttcagg cgagttaagg tatttgggct tttgatcttt
     6781 atcgagtaca cgtccgccaa agccaatcac acgaccgttt ctgtcatgga tggggaacat
     6841 aattcgatta cgatatctgt cataaatttt accttcatcg cctttaatga gcataccggt
     6901 actcaatagt tcacgttgat tgttcgggaa agctttttcc aggttgtgcc aaccttcagg
     6961 tgcatagcct aaatgatatc gtttggcaat ttccccacta aggcctcggt tgcgaaggta
     7021 gtcaattgcc acttgtccat tatattttaa ttttttttgg taatattggc tgacttctga
     7081 catgagatta tagagattga gagatgattt atgtttctct cctgtaccat cccttgggac
     7141 acttaaaccc aagcgtgtcg cgagggtttc aacggcatcc gtaaaacctt gattcagata
     7201 attcattaca aaactgatgg cgttacctga agcgccacaa ccaaaacaat gataaaattg
     7261 tttttttgca acgacattga atgatggtgt tttctcatta tggaaagggc aacacgcaat
     7321 atgactgttg cctctttttt tcaaaggaac atatccgtct attaattcca ccaaatcagt
     7381 gcggtttaat agatcatcaa tgaatggctg tgggattaag ccagacatat tggaactcag
     7441 cttaattttg cctttatccg ggcgctgact tgggccatgt ccgcgcgtcc ttgtaatttt
     7501 ggcttaagga tagccatgac tttacccata tcagacattt tttcagcgcc agtagacttg
     7561 atcgcgtctt tgattaactc ttcaatttca ttgtcagtta aaggttcagg caggtatttt
     7621 gaaatgacat tcaactcaaa ctgttcctgg gcaacgagat cgtcacggct tgctttttca
     7681 aattgagcaa tagattcttt gcgttgtttg gacattttat ccaggatgac taacatgcgc
     7741 tcatcatcca cttcaatgcg ctcatcgact tcaatttgtt ttaccgcagc actgattaag
     7801 cgtaaagcat tgagcaagtt tttgtccttt gcgcgcatag catctttaat gtcattgttg
     7861 atttgttctt ttatggtcat tttaattcct taaaaaacaa aaggtcacat acaatgtgac
     7921 cttggtgtaa tataggacga gctgcgattt actaccgaaa ctgccacgaa ggccaatttc
     7981 aaataaaaac aattaaaaat aacagagctt ccaaaacagg gcactgttca agtgcctcac
     8041 gcaaacttgc tttttagtgc agtcaatctt gaaaaaatca gatcaggtag tgcttttttt
     8101 acgtcggtga ccaacacctc gtcttgagct aacatcacga gatatttttt taagatgacg
     8161 ttttactgca gcagcttgct tgcgcttacg ctctgcggtt ggtttttcat aaaactcacg
     8221 gcgacgtaat tcggttaaaa taccggcttt ttcacaagag cgtttgaaac gtcgtagtgc
     8281 atattctggg ttttcgcctt cttttacacg aacggtaggc attaactagt cctctggtta
     8341 tttaatctta ataggtgggc aattctagtg ctagattttc cacacgtcaa gattaatcta
     8401 tacgtttctt gctattttag caaattatca agacattgtt ctttaattta atggtatctt
     8461 ttgtctgtga tataattgaa aaatgattaa aatctggaaa attgattccg attattgagg
     8521 tagttcatgc ttgtattggg tatagagtct tcctgtgatg aaacaggtgt tgctgtctat
     8581 gattcagaat caggattatt gttccacgcc ttgcattcac aaattgccac tcatcgtgta
     8641 catggcggtg ttgttccaga actggcatca agagatcatg tgaattactt ggttccttta
     8701 gtcgatgagg tactaactaa agcgcaaatt cgtaaaaacc aattggatgg aatagcctac
     8761 accgcaggac ccggattaat tggcgctttg ttagttggtt cctgctttgc caaaagtctc
     8821 gcctacgcct taaatatccc ggcattggca attcaccatt tggaagccca tctgttagcc
     8881 gcaaaaatgg aaaccccatc tctggatttt ccctttattg ccctgttggt ttccggtggc
     8941 cattgtcaac tgatagaagt aaataatatt ggtgagtatc gattattagg agacactctc
     9001 gacgatgcag taggagaagc ctttgacaag acagccaaac tgatggggat tccctatcct
     9061 ggaggagccg tgttggcaaa tttggctgat caatgtttat caacacccta tcaatttccc
     9121 cgtcccatga ctgacaggcc aggcctggac tttagtttta gcggactaaa aactcatgcc
     9181 ttaaatacct ggaaccaaag tgaaaaaaaa gaaagcgacc gctccgaaat tgcaaaagca
     9241 ttccaacaag cagtagttga aacactaatt ataaaatgca aaagagcaat taaagagtcg
     9301 caatcaaagc gccttgtggt agccggtgga gtaggggcca ataaagcctt gcgtagcgct
     9361 ttgcaaaaat ggattaaaga catcaaaggg gaagtgtatt tccccgctct ggaatattgc
     9421 acagataatg gtgcaatggt cgcttatgca ggatgtttgc gcatgatgcg gggggagagt
     9481 gatggggggt tggaggttat ggttaaacct cgttggcccc tcgcttgaac gcccgtcttc
     9541 ggcgcatctt gccagatcag ttctctaaaa aatgctcatg tacttatctg tacactccgc
     9601 ttttttagtc gcaacggctt gtcaacctgc gctcgaatac gggcgttcaa gggtaggtgt
     9661 cttcggcgca tcttgccaga tcagtgctct aaaaaatgct catgtactta tctgtacact
     9721 ccgctttttt agtcgcaatg atctgtcaac ctgcactcga atacgggcgt tcaagggtag
     9781 gtgtcttcgg cgcatcttgc cagatcagtg ctctaaaaaa tgctcatgta cttatctgta
     9841 cactccgctt ttttagtcgc aacggcttgt caacctgcgc tcgaatacgg gcgttgaagg
     9901 gcaagttgtc tttggcgcat ctttgcttgt gcattaataa gtgctgcgcc ttattccttt
     9961 tctttgactg ttttcgtttt aggtttggtt gatttcggtt ttttagttgt ttcttccttg
    10021 ctggctgtct tttttgcttt tgttgttgct tttttggctt ttggtttttt tacagcctca
    10081 gcttgttctg tcttttctgt ttcatcaata ttcgtgtcaa tgacttcttt gccaggaaac
    10141 tcttgctctg cagaagtagc tggagaagct tccattattt cctctatcac actgtgtttg
    10201 aacttgattt taggctcttt tccatctatc aaacgattaa aattatctct gtgtttgtag
    10261 agaaccagaa tggtgatcat gaataatggg gggaaaatat taagattacc caccaagatt
    10321 aaggagtaaa aaggggctaa agagatagaa gctattgatg ctaaagagga atagcgccag
    10381 aaatttgcta ccagtaacca ggtcgcggca accatgacac caattacaaa atgaaatgct
    10441 aacaaggctc ctattgttgt agcaacacct tttccccctc tgaaatgaaa gaaaactggg
    10501 tacatatgac caacaactgc agctaaagca gtaaaggcaa cggttacagg ttcggcactt
    10561 aaaaatttgg ccaggatgac aggaagtata ccttttaaag catcgaatac catgaccata
    10621 ataccgtact gcttccctcc aagacggagt acattagtag cccctgggtt ttttgaaccc
    10681 tcttctcttg ggtcggggag cccgaacgta cggcacacta tgatagctga attaatggag
    10741 cccattaaat aaccaactag tattagaaat ataaaaagtg ccaccgattt ctccttactc
    10801 gggaataatt tttattatca accaatctca attaacaggc aattttaaga tagaaaatat
    10861 gccaagacta gataatagaa ttcatttcat gttatttcaa tttaattcca tgtttagtgc
    10921 tggaataagt gtaggaattg acttgtttat gcaaacagca ggcatggttg gtataatttg
    10981 atccaacaag agcgttcata ttgctaaaaa agaggatgtt ccatagaatt tgaattattt
    11041 gcaatagttt gagacggata ctgaatgttg ctttttagtc ctgttcggtg tgtcactatc
    11101 attctattcc attatttaag ggaattttac taaagctatg gatgccattt atctcctttc
    11161 tgcaacagaa atcattcgcc gcattaaaat gaaagaactc tcggctgcag agatcatggt
    11221 ggcgcactta aaccatattg ataagattaa tcctgtaatc aatgcgctca ccgaacgtat
    11281 tccctctgag gaatgcttaa agcaggcaaa ggaaattgac aagagtatcg cttcgaaaaa
    11341 aaacctgaac aagttaatgg ggttgcctgt tgccataaaa gacgctttgt atgtgaaagg
    11401 actaatttgt tcttcggcat gttcaggatt ttacaaggga gaaaaagcag tacgtgatgc
    11461 gaccctggtt tctcgactga aaaaggaagg agcaattatt cttggcttaa ctaatgttcc
    11521 tgaattgtgt cgcggtggtg attcggataa tttaatctat ggacggacca ataatcctta
    11581 tgatctggca agaaccagtg gaggcagtag tggtggttcc gcggctttga tcgctgcagg
    11641 aggcgttcct tttgctttgg gttccgatgg tggaggtagt ttaatgcagc ctgcccattg
    11701 ttgcggcatt gttgcattga agccaacaca tggccatctt cctcatacag gctctgtagg
    11761 tggggattcc tatggtctta ttggcaatct gatttctttt ggaccaatgg ctcgttctgt
    11821 cagtgacctt cgtttaggac tctcagtgct tgcgggctct gaccaatatg atccttatac
    11881 aaatccggtt cctgttatgc ctgctgcacc actaaaaaaa ttacgtgtag cctattttac
    11941 agagaatggt tttacgccag ttgatgcgga gatacagaac gttgttaaat cagcagccct
    12001 ggctttgcag gatgatgtgg ctatagtgag ggaggtacga cctgattgtg tcagcaaggc
    12061 ttttgatttg cactgggagc tttttctcgg cggcgatcgc ggagcgggat ttaaaactat
    12121 gttatcggag ttaggggtta acaatctgtc atgggaatta caagagttct tgcgccaagc
    12181 ggaacaaact caattctcgg tgactcaatt gcatcaacgt atgcgtgaaa ttgatttgtt
    12241 tcgcctggaa ttagctttat ttatgcaaga ttatgatgta ttgattagtc cagtgtttcc
    12301 aacagtagca aaaccgcatg ggattgggat taaagaaatc agcgattttt cctatgcgat
    12361 gacacacaat ttaagtggat ttccaacgat tagcctaaga tgcgggactt cagccgaggg
    12421 gctgcccatt aatgtattaa tagccgctaa tcgctggaaa gatactacct ctctggctgt
    12481 tgcggaacgc gttgagcaat taatgggggg atataaaccc ccttctctaa tccactctct
    12541 gcaatgaagc aagaatcaat aatttttgaa acagcaaact agggcagaaa caaggcttgc
    12601 aaatcattag tatattgcct gcctaatggt gttacttgcc aatgagtatc tgttaaatta
    12661 atcaattgtt tcttattggc caatttcagc aagggtaaaa gctgattaag ttccaatcct
    12721 gtcgctgtcg taaataactc caaaggtatg gattgttcca gccgggttgt attcagcata
    12781 aactcaaaca acaattcttt acctgtaatg gtttcgcgag cagccaaata aggtttctca
    12841 ttatccagat aatctttggg ttgacggtgc ttacgggttc ttattattgc gtttggggtt
    12901 gtcatttttc catgagcacc agcgcctata cccaaataat caccaaatag ccaataattg
    12961 atattgtgtc tggcttgctt ttctggtttg gaaaaggcag aaatttcata gcggtgatat
    13021 ccggaatcat gcaacagggc aaatccttgc tcctccagca agtaatcttc ttcttcagaa
    13081 ggcaggggag gagtatgctt gtaaaataca gtattcggtt ctatggttaa ttgataccag
    13141 gataaatgct caggttgata ggataaagcg ttcttaagat cctgtaatcc ctgcgttaca
    13201 ctttgattag gtaaactatg catgatatca agattgagat tatcaaagcc ggctttacgg
    13261 gcagtatcta tagcagaatg ggcttgtttc tcgtcatgaa tacggccaag aatttttaaa
    13321 tgattgggat taaagctttg aattcccaaa gaaagacgat tgattcctga ttgtctataa
    13381 tcagtaaatc gatgttgttc tacagtaccg ggattggctt ccattgtgat ttcaatattt
    13441 tttgcaaagg ggagtatgcg ctttaattca ttaaataagt tgttatatgc ttcggctgaa
    13501 aataaactgg gtgtgccgcc accaataaaa atggagttaa tttcacgggt attgtatgag
    13561 gaaatatccg ttttaaggtc ttcaatcaaa gcctgaatat actgatgttc gggtaaaaca
    13621 tcagggcttt tgtgtgagtt aaaatcacag tatgggcatt ttctgatgca ccagggaata
    13681 tgaatgtaca aggataacgg aagcattaca atttttaaac tggcttgtta gggaatgcaa
    13741 tagtatcatg gtagcaattt tgctacaaaa ttaagtgatg accattgatg cgaaataaac
    13801 caaaatgtaa gcaaagaacc gtgatagcca aatcccggtt gtttacagtt gaagaaatgt
    13861 atctggaatt ttcgaatggg gctcagcgag tctatgagcg gattaaaagt catggtcatg
    13921 gtgcggtgtt aatagttgca ttgactcccg gagaatcatt attattagtt cgggaatacg
    13981 ccgcaggtac tgattcttat gaattatcat ttcccaaggg agtgatagac agaaatgaaa
    14041 cccctataga agcagctaac agagagttac gtgaagagac tgggtttgcg gcaagggaac
    14101 tgcactggat tcgttccatg tcattggctc ctggttattt tggagctcgc attgagctta
    14161 ttgttgcaga gggtttgtac cattcgccgt tgccaggcga tgagcctgag cctcctgaaa
    14221 cagtagagtg gcctttacac gctgctcatg agttattgga aaaaccggat tttaccgagg
    14281 cgcgcagtat tgctgcgcta tttttagtta aagagtggtt gaataagagg aaatcatatg
    14341 acaaataatc gagtaagagt tgctgtcaca ggtgcagcag ggcaaatagg ttatgccctg
    14401 gtgtttcgta ttgcttccgg tcaaatgttt gggccaaata ctgaagtgga attgaattta
    14461 cttgaacttg agccggcact tccctcactg gaaggagtcg ctatggagct tgatgattgc
    14521 gcatttccct tattaaaacg cattgtttgt actgcagatt tgaataaggc aatggatggt
    14581 gttaactggg cattattagt tggttcagtt ccacgaaagc aaggcatgga gcgttctgat
    14641 ttattacaaa tcaatggtgg tatattcacc aaacaaggtc aggcgattaa tgattatgcc
    14701 agcgatgatg tacgggtatt tgttgtggga aacccttgca ataccaattg cctaattgct
    14761 atgaatcacg caaaagacgt gccttctgat cgtttttatg caatgaccac attggatgag
    14821 ttaagagctc gcactcaatt ggctaaaaaa gcagacgttg acattactgc agtgactcaa
    14881 atgacgattt ggggtaatca ttccgctacc caatatcccg atttttataa cgcaaaaatc
    14941 aatggaactt ctgctgcgca agtcattaat gatgaaacct ggttaaaaga gacttttgta
    15001 tcaaccgtcc agcaacgagg agccgcagtg atcaaggcac ggggttcatc ttctgctgct
    15061 tctgccgcca atgcgataat tacaggagtc aatcatttgg ttactgatac tcctgctggc
    15121 gaatcgtttt caatgtgccg tcgctcaaag ggagagtatg gagttgatga aggtttgatt
    15181 ttctctttcc catgccgaag agaacatggc gaattaaaag tggttgaaaa cctggagttc
    15241 aacgattttg gacgcgagag attcaatacc actttaaacg aattacgaag tgagcgtgac
    15301 acggtcaagt ctttaggctt attggattaa ccaaaccggg tcaattcttg attgatttga
    15361 ttctgttgtt tttgctgatt accctgcttt gtgaattttt cagggcaggg tgctgtcgca
    15421 ccttgtttta taaaccaggc ttcaatgact cttttgtttg taaatcaggg tctttattgg
    15481 gatgttgatg tctgctttta tattcctcac tcaaatacat ttgaaataat gtcgaattct
    15541 ccaggatggt tttggcgtct gtatttcgta aaagagttga tacggaacga ccattggata
    15601 atatttcgtt gagagtatga atcaattgct ctatttgctc gaagggttct tttcttttat
    15661 gcatcaaaac gggaatatgc ttaattatag ctctggcaag ttgttcgtct gtcaagtaaa
    15721 ggattaaacg ggtttgagaa attttgatga aatttctttc tgatctgaga atgatattgt
    15781 ataagaaatc aggatctttt tttaagtgat tgctcaattt ttggctaaat ccaagcctgt
    15841 cccggttgtc aaccagtgtt tgggttaatt cagtatgatc caattgacta aagggttttc
    15901 ctatcttttt ttcaagccag gtcattaaat catagtcatg atgagaatga ttcagtgcgc
    15961 tgaattgaaa tcgtggcgat acttttggat gagataagac ctgattccag tcaatagttg
    16021 gcatgtggtg cagtttagcc agagcatcca tggattttct gattacatta aaattaatcc
    16081 gccatttttc aatataaggc aaggcttctc taattgctct aagcagttca ataaaagtat
    16141 ttctggcttt tggattgtag ttctcggtgg cttctttaag atgttgaact gctggaaaag
    16201 tatacttgcc atttctcatt gcttctaaat gaaatactga ttgtaatgat tttagcaata
    16261 cagaaaaagg gtcttccata ccaatattcc tccacaggcc cttctttaaa agtagtttat
    16321 aaaaacataa aagccaaata ttttgcaaaa tggattaata atttttagat tgattgactt
    16381 tatttatcgt tttgataaaa acaattgatt ttatttatta aatgatttca cctaaattta
    16441 acaatgaaaa tagtgttaat ttaacgagga gaaaacttat gattacggta ggcaataaat
    16501 ttccagagtt tcaacttaaa gcaacggtag ctaatgatat aaacaatgca tttcaaatga
    16561 tctctaatga aacgtatcgg ggtaaatggt tagtcgtatt tttttggcct aaagatttta
    16621 cctttgtctg ccctactgaa attgcggagt ttggaaggct aaattccgag tttgccgatc
    16681 gagacgctca aattctgggt ggaagcacag acagtgaatt tgtacacttg gcctggagaa
    16741 atcagcatcc ggacttaagc aatttgccat ttcccatgct ggctgatgtt aaacgagaat
    16801 tgacagcaaa tttgggcata ttggatgacg aggagggagt tgcccaacgt gcgacattta
    16861 ttgtagaccc tcagggagtt actcgttttg tgatggttac ggatttaaat gtagggcgca
    16921 atccagctga agtattacgt gttctggatg ctttacagac cgatgaactt tgtccatgta
    16981 attggaaaaa aggtgaagaa acaattcata tcaaataaat tgaaaaaaga ggcatcaatc
    17041 atgttagaga atgttaaaga aagtataccg gaagtggcta aagacatacg cttgaatttg
    17101 actaaggtac ttgatctgga acaaagtgat ggattgagtg agcatcagat tttaggttct
    17161 gcattggctg tgagttattc tttaggcaat gaggttctaa ttaaaggact taaagaattg
    17221 gtgcataacg aacagattgt gcgtgcagca caacttgcgg caagccttat ggcgatgact
    17281 aatatttact atcgctttgt tcatttaact gaaattaagg aaattgagca cattcctgca
    17341 ggattgcgaa tgcaaggaat gatgactcca ggggtagaca gggtaacctt tgaaatattg
    17401 tcccttgctg tttctgctct caatggttgt ggggcttgtg tcagttctca cgcacgacaa
    17461 ttgaaagagc acggcttatc cgatacaagc cttgcacgag ttggacgtat tactgctgtg
    17521 attcatgcgg ttcatgtgag tgctcgaatc aaataaatca ttgcatttga agcagattat
    17581 ctgcttcaaa tgtaaaaaac tatttaatga cgccgcaagc tatacggtcc cccccgccac
    17641 cttgtggagg attgtcactg taagtgtctc cattggcatg gatcatgaca gctaaattgt
    17701 gcatatcatt taatttcaaa cgaggagcta aagtaggtat cattgctttc ccattacttg
    17761 tgacatataa aacaggtaaa tctcctaaat gcccgttacc ataagggcct tggtggctat
    17821 tggtattttg aggatcataa tgtccttctg catgcattcc gtggttacca caatccgcat
    17881 ttttatgtaa atggaaacca tgcgggcctt caggaaggtt agttaaatct ggtgtaatca
    17941 aaacaccata aggggtttgt gtaaaggtta ccttgcctat tgcaactggt ttgggaccag
    18001 tcgtgtaaat tggtgctgta agatcatcgg caatagccat gcttgagaat aaaatagtac
    18061 caattaatat tattcctgat ttattcatgc catttcctgg tatttttcaa agcccgttaa
    18121 tatttttagt attatccaag aaaaacattt attccacagg atttaaaaaa aattcgattt
    18181 ttttgggtta aaaatatttt taattcagac atttaatata tcaggatttg cacgttcaaa
    18241 ccttcattgt gtagaccatg agagtttcgt aaactctatt ttaaagttta gagagaggat
    18301 tacaaattaa ttgaatcggt aaaaaatgat tgctgtataa taagcaaggt ttatacttta
    18361 tgttttaagc tttaattaaa cagagagtcc tcatggaatt gagaattgat aatacccctt
    18421 ttaaagccat ttttcagcct ttggatctgg gctttaccca aattaagaat cgtttattga
    18481 tgggttcaat gcacactgga cttgaggaag ataaagaaaa tttaaatcgc ctggcaagct
    18541 tttatagaga aagggctcaa ggtggagttg gattaatcgt aacgggtggt tttgctccta
    18601 accgagctgg ccgtctggct cccttcgctg ccaagctgac taatagtaaa gagcaacatc
    18661 ggcatgaggt cattactcaa gcggtccatg aagcaggcgg aaaaattgcc ttgcaaatat
    18721 tgcatgcagg gcgttatggt tatcaccctt ttattgtagc gccgagcggc attaaatccc
    18781 ccattagccc gtttaaaccg tgggaaatga gtaaaagacg aattatcaaa actatcaaac
    18841 attttgcccg atgtgccaga ctggctcaat tggctggcta tgatggggtt gagatcatgg
    18901 gaagcgaagg atatttgata aaccagttta tagtcactca taccaatcat cgaagggatg
    18961 aatggggagg aagtttctct aatcgcattc gatttcctat tgaaatagtt cgcagtgttc
    19021 gtgaggcggt aggcgagcac tttattatca tttatcgctt gtctatgctt gatttagtgg
    19081 ctgaggggag taattggcag gaagtggtta ccctcgctaa agccattgaa caagctggtg
    19141 ccagtttaat caatacgggt ataggttggc atgaagcaag aattccaacg ataggaacga
    19201 tggttccatc tgctgccttc actcaagtga cccagcattt aaaaccggaa attaaaatac
    19261 ctgtaattac atctaaccga attaatacac cagaattggc taatcaatta ttagaagatg
    19321 gggtcgcaga tatgatctct atggccagac catttctagc ggatccctgc tttgcagaaa
    19381 aagcaaaatt aggcgaaagc aaagcgatta atgtatgcat cgcttgtaat caagcatgtc
    19441 ttgatcaagt ttttgtgaac aaaacagcat cttgcctagt taatccccgc gcatgcaacg
    19501 agacaacctt ggtttatgaa acaacaaatc aaccgaaagc tattgctgta gttggttctg
    19561 ggccagctgg ccttgctttt gctgtaacag cagctgagcg agggcataaa attaccctgt
    19621 ttgaaaaagc gaatcagatt ggcgggcagt ttaatttagc gaaaaaaatt cctggaaaag
    19681 aagagtttca gcataccttg aattattttg cctaccaact ccaaaagtat aaagtggatg
    19741 tacgtttgaa taccgaagct actgtggaat tacttaaaga ctatgatgaa gtggtcttgg
    19801 ccagtggaat tatacccaga gttcccgaaa ttaaaggtat agatcataaa aaggtggtga
    19861 gttatattga tgtgattact caaaacaaac ccgttggttc acgagttgcc gtcattgggg
    19921 caggaggaat tggttttgat gtcgctgagt ttttaactca tgatcatcaa ttgacttctg
    19981 agcaatttta taatgaatgg ggaattgata ttagcgggca gcatagaggt ggattaaaac
    20041 cacctgaaat tgcaccgagc cctcgtgaag tgtatttatt acaacgcaaa aaagaaaaaa
    20101 tgggtaaacg attgggaaaa actaccggat ggattcatcg ctccagttta aaacataaac
    20161 aagtcaaaat gttttcgggt gtaaattacg agcgtattga tgatcaggga ttgcacttga
    20221 ctattgacga caagcctcaa gttttggaag ttgattctat agtgatttgt gcaggccaaa
    20281 tagaattaaa gaatttgtat gagcctctga aaatggcagg caagttggtc catttaatag
    20341 gaggcgctta taaagctttg gagcttgacg cacgtcatgc tattgatcag gcttgtcggt
    20401 tggctgcatt agtatgagta aaattgctgt agtgataaat cctatagcag gcggcggtcg
    20461 cggaaaaaaa atttggaaag tcctaaaacc cgggttacat gccctattcg aacaaactgt
    20521 ttacagaata tctaacaggg ttaatgatct tgaagcaatc acagacggac ttcttgctga
    20581 aaaccctgac agcctgttaa taatcggtgg agatggcact ttaaatcatg ttttgaatgg
    20641 cttaattgag aacgataaat taaaaaatcc gcaaatcaaa atcgcctttt ttaacgcagg
    20701 ttgtggcggt gattttacaa gacaatttcc ccaacagaaa atgaccgaat tcctggacag
    20761 attaagtcat aaccaattta tcaagtgtga tattgggaaa attactcttg ccaatcaagt
    20821 gtcgaggtat tttattaata ttgccagctg tggtctgtca ggacatgtgg ttttgcgcgt
    20881 atctaaaagc aagtggttaa aaaaactggg cggtactctg aattacttgc tgcatgcttt
    20941 aaccgggttg atgacctatc gtaaaaccaa agtaagggtt cagatagacg atgctctacc
    21001 gtttgatagc tccatcctgc tcatggccat ctgcagcggt cagtatttcg gcggaggtat
    21061 gcatgtagca ccaatggcaa aagtgaacga tggcttgttt gatgtggttt cttttaatga
    21121 attttcaaaa ctaaatgcac ttttaaagtt atataaaatt tattcagggg ggcatttgtt
    21181 ggataataat gttcattatg ttcagactaa aaaaattaaa atagagccgt tggaagaaag
    21241 taaaatagaa attgaggcag atggtgaaat tattggttgc ttaccggcac agttt
//
LOCUS       DATNSN010000038        20835 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.038, whole genome shotgun sequence.
ACCESSION   DATNSN010000038 DATNSN010000000
VERSION     DATNSN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20835
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.038"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            108..770
                     /locus_tag="VAH76_001208"
     CDS             108..770
                     /locus_tag="VAH76_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385851.1"
                     /translation="MSKEQIKKTALEIVEYIHGNIKACNIPNQKRENKFFAPLVYLCQ
                     TECDKILSNTKISLLERLLKCAKIVDDLQSGNCMQQAFLAFQRLLTRLIEDKLSNFST
                     CIPISIMTISKHAFLIIDNDIVCDPWLNFVGDLRDYRFAKMKRKEYFGIRSDWTCFTN
                     TEVYDEDSTEFTIQFVNALGVGISGFSEVKLANQLMQSSSMASDLGEDPVISIPKEFH
                     QI"
     gene            878..1651
                     /locus_tag="VAH76_001209"
     CDS             878..1651
                     /locus_tag="VAH76_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1385852.1"
                     /translation="MNFLNNKIVMITGASSGIGQACARLFAAQGARLILAARRVERLH
                     ELSSELKHHNNQEHYILPLDVCKKDLVRQQIESLPGQWNSIDVLINNAGLALDTLPLH
                     QGILEHWDTMIDTNIKGLLYISRLILPGMLERNSGHVINIGSIAGHECYPNGNVYSAT
                     KHAVHAISKSMRLDMLGSAVRVTEIAPGAVETEFSEVRWKDKQRAIDFYKDFQPLMAE
                     DVADAALYCATRPLHVDIEQMIIMPTVQASANHLSRKGR"
     gene            2170..2400
                     /locus_tag="VAH76_001210"
     CDS             2170..2400
                     /locus_tag="VAH76_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385853.1"
                     /translation="MNKLIVAVTAGASLLLLTGCAVDGVAVDDVSYDSGYSNDYVYSV
                     GYYSNRPYWGNNYYSSPSFVGPYGYWGGDYDF"
     gene            complement(2454..3023)
                     /gene="efp"
                     /locus_tag="VAH76_001211"
     CDS             complement(2454..3023)
                     /gene="efp"
                     /locus_tag="VAH76_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO1385854.1"
                     /translation="MAVYSTNEFKNGLKVMVDDAPCSILDCEFVKPGKGQAFTRIKIR
                     NLKTGRVVERTFKSGDTLPSADVADVEMQYLYNDGEYWHFMVPDTFEQYAVTENVLAD
                     AAQWLKEQDVCVLTLWNNEPIQVTPPNFVILAITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTGELIKVDTRKGEYVSRAKE"
     gene            3095..4075
                     /gene="epmB"
                     /locus_tag="VAH76_001212"
     CDS             3095..4075
                     /gene="epmB"
                     /locus_tag="VAH76_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEO1385855.1"
                     /translation="MRDTSLSWQKILAQGFTSTADLLDFLELPRSEGNLFAEKQFPSR
                     IPLGFAERMQKGNPKDPLLLQVLAKEDELTVADDYVIDPLSESNTLIKGLLHKYHGRV
                     LLTLTGVCAVNCRYCFRRHFPYQANNPGRRGWKEVCAYIANDPSITEVILSGGDPLLA
                     ANLVLEELLQSLEEISHVHTLRIHTRIPIVLPERIDKGLLDLLTNTRFKKVIVVHCNH
                     PQELDESVLRACSDLKKATCYLLNQSVLLAGVNDDAVILSKLSHALFDYGIMPYYLHL
                     LDKVKGSAHFDMPLPRAQSIYHQLQSLVPGYLLPRLAREEPGRSSKTLLI"
     gene            complement(4144..6216)
                     /gene="ppk1"
                     /locus_tag="VAH76_001213"
     CDS             complement(4144..6216)
                     /gene="ppk1"
                     /locus_tag="VAH76_001213"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEO1385856.1"
                     /translation="MDTTLDNPDYYINREFSAVAFNQRVLMLANDERVPLLERMRFLS
                     ICSSNLDEFFEIRVAGLKEKIAMSSGKLTIDGLRADEAFSQISHKAHALIDQLYNIFN
                     KQLLPALRKENIHFLETDQWTDDIHLWAKHYFKHEILPVISPIALDLAHPLPRLINKS
                     LNFIISLSGKDAFDRNINYAVVHAPRSIPRMIHLPSELCGDAHYFVYLSSIIKTHINS
                     LFPGMEISGCYSFRLTRNSDLFLREEEIDDLAKAVQREIFSRHYGHVVRLEIENKCPE
                     KIVDFLLQKYHLRHEDTYYCDGPVNLQRYMSAINSIDRPDLNYPAFTPQYPKFSKSQR
                     NLFNILDEQDILLHHPYQAFDLVIDFVRQAASDPNVLAINQTLYRTHSESVMVDALVN
                     AAHSGKEVTAVIELRARFDEESNLKLANKLHAAGILVLYGVVGYKTHAKMTLVVRRTH
                     GKLKRYVHLSTGNYHEQTAKRYTDLGLLTSEPTITSDAQLIFQQLTGLGKVVKLKALS
                     HSPFTLQKTLLQFIEQCSTAATGGIDTEIILKVNGLTDKVMIQALYKASQAGVKINLL
                     VRGVCCLKPGLPGVSENIRVLSFIGRFLEHHRVFYFRINEEEHYFCSSADLMERNLYN
                     RIEIMFPIYDEGCKKRIKNEIIKNYLKDNNNVWEMQSDGTYKHLMQNGGCAQEKLIAL
                     YEEEDKSI"
     gene            complement(6257..7462)
                     /locus_tag="VAH76_001214"
     CDS             complement(6257..7462)
                     /locus_tag="VAH76_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1385857.1"
                     /translation="MKRIFFVVFLLCLETACNTAKNNPLPAQLSDKALVPGFKEVRTE
                     FTRSDDPIIKKQSLERVKQLAKAYPDTMFHQKGSFSLLIISGGGDYGAFGAGLLNGWT
                     KSGSRPTFTTVTGISTGALIAPLAFAGPKYDATLKKVYTTITTKDVLSNKPVLWTLLT
                     GGSALKGTKPLQDLIARHVDEKLLDDIAKGYYQGRRLYVGTSNLYSRKLVVWDMTRIA
                     ASKNPERVQLFRKILLASASIPVVMPPVYFDVMANGKPYTEMHVDGSTTFAVFLSRLG
                     SDIKTAKKHFKLKQKPNVKIYVIRNNQNQFHYKVIKPKLLVIGKRAIESVTASQGAAD
                     VIFIYLCALIEGVDFNLANVPNEFDSNSEELFDTQRMNQLYQIGYSKAVKGYPWQKIP
                     PDVRALHGD"
     gene            complement(7538..8071)
                     /locus_tag="VAH76_001215"
     CDS             complement(7538..8071)
                     /locus_tag="VAH76_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEO1385858.1"
                     /translation="MIKTFIEIIYSFGKIGLISLGGGNSMLKLLEYEAVTYRHWIGKE
                     EFVGMLGSSFLFPGLTGIKLAALIGYKAAGITGLILAIICLNLPGLIMALVGYQWLNS
                     HNGPITRKIMISVQYGALALLAAATFSVVQGVIDVYYSIPMAICCILFFLALAFWNLS
                     PFYGFLAFIAVCFFVVR"
     gene            complement(8068..8688)
                     /locus_tag="VAH76_001216"
     CDS             complement(8068..8688)
                     /locus_tag="VAH76_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385859.1"
                     /translation="MQRLVWNFEFSSEQTTPLTTLDHDAQDNLKWEKRYFWPADQIIR
                     LNTIDSSLLDLANYQKKHKEDYYYLLPDSDHNIKQRRNELLYKPVIKQSGSALGFGPK
                     VHLENPQTLSCHGDAGQLNRQELLKKIKEKGIVVFVKKEAFVFKFPTTPTVKLELARL
                     EVKDKVYFSACIEGRSLKLVETISVLLFGTQPSFEYVTFLKKTLKI"
     gene            8864..11302
                     /locus_tag="VAH76_001217"
     CDS             8864..11302
                     /locus_tag="VAH76_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEO1385860.1"
                     /translation="MAIVFFLIYLAFTLVVLSRRINPLIWEIGSIIYLIPATFYFGFP
                     WIIGLFIWFFIIAAVLVVRVEPLRFFIGDYLYKTAGKSVPKLSKTEEEALNAGDTWLE
                     QDIFTGKPDWQRLSAVSTELTAEEQAFLDNETHTLCSMLDEWEISQEYDLSPKVWNYI
                     KEKGFLGLVIPKEFGGKGFSARAHSDIVMKIATRSRVAAVTVMVPNSLGPGELINYYG
                     TDEQKAYYLPRLAKGVEIPCFALTEPGAGSDATSIQSEAIVVKKKIDGKSVLGLNITL
                     DKRWITLAPVATLIGLAVNLKDPDGLLKGEGEEGITCLLIPRDTENLEIGNRHLPADQ
                     PFMNGTLRGKDIFVPIDTIIGGQKKAGSGWQMLVECLSIGRSISLPALGAGSSSISYL
                     TTGAFARIRRQFNVEIAQFEGVEEKLAEIAGLNYLINSTRLLTVAAVNEHKKPSVASA
                     ITKYFNTELARITVNASMDVHAGRAIVVGPRNYLNSLYQSVPISITVEGANIMSRNLL
                     IFGQGSMACHPFIRDEFYAISSENKEAFREIIWKHIHYFMQNFAKTVCSAWTSGLFIA
                     VPENSMKREYKKLARLSYAYSWLADLSLIYLGGDLKRKERLSARLADGMSYLYMAMAA
                     LRNVQLNQDHPDEQLHARWAVSYCFYHAQKSMIALCHNFPSKFLGFIARVVAFPFGQT
                     MRYPSDKLDHQLARLMTGNNHYRDRLKKSVFLSGDSKQPVDRMEHALQLIIEADGLSK
                     KVSDLKRYKWSKLKGKLKEKVESGQLSQQEMEELLAVEDARWDAILVDEFTFDSMKKK
                     TLHSVIEKMKSPLA"
     gene            11416..12108
                     /locus_tag="VAH76_001218"
     CDS             11416..12108
                     /locus_tag="VAH76_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1385861.1"
                     /translation="MPKYTLPTRDALLKAMQVGETSIEAAEYMATRFEQILTQAKLLP
                     ECNDMLEKIQEYAQFVKFKLLSSAQVWSGQERPISDYQNMQENKAEFLASHLKELPSG
                     LKLEIAIGDDAKILRGFSSNGKMVEGEQLKTMDGLLEGWLAKNNLAISGGAVVQRNST
                     GNQTSVDPEEIRKLINDSEKGVAKYFADKGVSMEVVQRTYQEPKALETKREEIRQEIE
                     SGAEAPTTQSIR"
     gene            complement(12145..12807)
                     /locus_tag="VAH76_001219"
     CDS             complement(12145..12807)
                     /locus_tag="VAH76_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO1385862.1"
                     /translation="MKTAMILAAGRGERLRPLTDKIPKALCTVRNKPLIEHHIINLAN
                     AGFDRLIINHAYLGGQIRQYIGNGKQWGLNVIYSPEPPGGLETGGGIVNALPLLGEGP
                     FLTVNADIYTDFDFTKLQLKNIDTFHLILIPKNPSLLHHGDFGLINGSHLTNTNQAYT
                     FSGIACYNPKVFANCRQGRYSVTPLLRKYVEQNSATGSVHSGIWYDIGSFDRLHAANQ
                     FP"
     gene            complement(12804..13781)
                     /locus_tag="VAH76_001220"
     CDS             complement(12804..13781)
                     /locus_tag="VAH76_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEO1385863.1"
                     /translation="MHERENALKEWLAQTIQQKDFVLLPLAGDASFRRYFRIQYNGLT
                     QVVMDAPPEKENIESFLHIANVLDKIKIPVPDILAINKREGFLLLSDLGDQLLLNKLN
                     HETVDNYYNQAMNLLLQIQKCPTDDPELPLFDKSFMLKEMNLCLEWFLKAYLSLDLKQ
                     DELQLFQHTIEWIASEVATQPRVFIHRDYHSRNLMLVKNKDSCLATIDFQDAMLGPVT
                     YDLVSLLKDCYISWPREKVLEWITYFHEKSPHTNIYSLTDFIRAFDLCGLQRHLKVLG
                     VFCRLYLRDNKAGYLGDLPLTLKYALECAETYEELHPFFNFLQKRVYLP"
     gene            14024..16543
                     /locus_tag="VAH76_001221"
     CDS             14024..16543
                     /locus_tag="VAH76_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEO1385864.1"
                     /translation="MAIGITACVLSLINYQGLAYSAALINEPIQACVIAREVDLTDDI
                     RTKFAQCLGWQADQSSPVCLGFYKPITVTPLASPDEVRILADTASFYRTQRSTLSGHV
                     EMQQGQRVVNAQTAYVYRDPKTNEVTKIEFLNHVRYLEPDRMMIARKAVVYPQDKSGE
                     VEDVLYRFNTNRSNALLPAWGRASLIKRFANQDYFLKEATYTTCAPQDKAWAIEAESI
                     SIDNEKGKGIARNAKLRIHEWPVLYTPYLSFPTNRDRKSGFLMPIVGYSNVGGADLGI
                     PYYWNMAPNYDMTLVPHLYTKRGLMLGGQFRYLTSKSTGTFNGNFLPKDKAFGRFLQD
                     NEVEFPQIRGLSTNRWEVNFVDSTQFLSDLQLNVNFQQVSDDYYLQDFSTNLASVTQR
                     QLLRQADLTYTTENWTFRGMGQSYQTLHPINEIPVSPVYERLPQLMARGYYDDLPFNA
                     QFNILGQYDQFHWPNDSWNIALNNMPQGPRFHLNPILSVPMMKPWGYVTPSVQFVENY
                     YDISRNYTWGTSRANYNLTIPRYSLDGGLYFERDLHLKGTYYIQTLEPRLFYLRVPYY
                     NQTLIPVYDSGFMIFNVDQLFRTNRFSGFDRIGDANQLSYALTTRWLEDESGAEKANF
                     SIGQIKYFSERRVQLCQSPTGFCTDNPDTFGNLSSTFGTSPVASRAVYKFNPAWGITG
                     DYIWDPATRATNNADLNLHYQPARNAIINGGYSYLVNGDVTQVRNNDTENNALHQAIL
                     SAAWPLSEKWSGIGAYSYNISKNYSMMSFLGVQYDSCCWAMRILGGRTFRSLNEEFEP
                     QYNNNIYLQILLKGLGSVANSDPSGILNTYIPGYYDPFRRR"
     gene            16719..18008
                     /locus_tag="VAH76_001222"
     CDS             16719..18008
                     /locus_tag="VAH76_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO1385865.1"
                     /translation="MFKRIALVCALFSGICFAEGKQLLDKVVAIVNDNVITSSELNAQ
                     VELSKKQIIAQNMQMPDESVLRKQVLQHLIDVDLEMQMAKQNGITIENAEIDEAIEKI
                     AASNHLNLSQMRDEITKQGISWQEYRQNIRKEMLISRVQQKAVGKDIIVTNEQVEQYL
                     KTAGRIENSNLTYHLKNIVIPLSEEPTTKQLQRAKIEAENLLNKIKKGEDFSRLAIEE
                     SSGEFALEGGDLGERHLAELPEVFAKEVVHMKVGQVAGPIRAGNGFHLIKLVAVGGEN
                     QRHVITQTHVRHILLKPDASMVPSEAIKQVNNIYRQIQSGKDFALMAKQYSLDAASAV
                     KGGDLGWVNPGELVPEFEKTMNSLPLHKVSKPVKTQYGWHLIEVIARRQKDDSEAFKK
                     QQVRQFLQQRKFVEAVQNWQQHLRSQAYINIVDKDLA"
     gene            18005..18979
                     /gene="pdxA"
                     /locus_tag="VAH76_001223"
     CDS             18005..18979
                     /gene="pdxA"
                     /locus_tag="VAH76_001223"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEO1385866.1"
                     /translation="MNPLLISSGEPAGIGPDLCLALAETDLPVVILGDLSLLEARASE
                     LNLSIKFLEYGPHQSFEKKAGYLTVWPVPCAAPVISGELNPQNAAYVMELLTLGASLC
                     SKGEFSALVTAPVHKANINAAGITFTGHTEFFADFFEVETVVMMLACSQMKVALVTTH
                     LPLRMVPDAINSLLIIKVIQQLHHSLKHDFGIQSPRINVAGLNPHAGESGYLGREEIE
                     IITPALNTLKNQGIDVLGPLPADTMFITNNINHCDAYVAMYHDQGLPVLKYAGFNEAV
                     NITLGLPIIRTSVDHGTALELAGKNKANPGSMLAAVKMAKDMALTRIK"
     gene            18981..19472
                     /locus_tag="VAH76_001224"
     CDS             18981..19472
                     /locus_tag="VAH76_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO1385867.1"
                     /translation="MSVISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIM
                     GRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSLGQAIKETTGASEVMIIGGAKIY
                     SQAISMANRLYITRIHHQFTADVFFPKINELVWSCRSKEYRQHDDKNQYDMTFCIYER
                     ISI"
     gene            complement(19479..20024)
                     /locus_tag="VAH76_001225"
     CDS             complement(19479..20024)
                     /locus_tag="VAH76_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HEO1385868.1"
                     /translation="MSKLVYLLLMIFFLNPAWANTHYHQHPVSPLANALRTGTDTNGN
                     ALYLCKAKLFNSIQPGKTWAGYGRCNVPYGGKEYVLSQFTIPNQNEFGRYSWEPNVEH
                     ALLMGKDTNGNPLFVCQSNFNGSIQPGKTWPGYSHCNISYGGREIITDNYRILSRPTE
                     IIVRTQPPVHRHPSQNRHYHY"
     gene            20312..>20835
                     /locus_tag="VAH76_001226"
     rRNA            20312..>20835
                     /locus_tag="VAH76_001226"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6311 a         3784 c         4402 g         6338 t
ORIGIN      
        1 ttaacctaac gggatatgtt tttatcaaat tgtaagcgat ttttgattta aaaaaggtat
       61 aatagataac aaaataaata ccacctgcgg ttagaactgg aaaattgatg agcaaagaac
      121 aaattaagaa aacagcactg gagattgtcg aatacattca tggcaacata aaagcgtgta
      181 atatccccaa tcaaaaaaga gaaaataagt tttttgcgcc tttggtttat ctctgtcaaa
      241 cagaatgcga taagattttg agtaacacca aaatcagttt attggagcgt cttttaaaat
      301 gcgcaaaaat tgtcgatgat ctgcaatcag gaaattgtat gcagcaagct tttttagcat
      361 ttcaacgttt attgacgcga ttgattgagg ataagctatc taatttttcc acgtgcattc
      421 caatttcgat catgaccatt tccaagcatg cctttttaat cattgataac gatattgtgt
      481 gtgatccctg gttaaatttt gtgggagatt tgcgggatta tcgctttgca aaaatgaaaa
      541 ggaaggagta ttttggaata cgttccgact ggacttgttt taccaataca gaggtctatg
      601 atgaagactc tactgaattt acaatccaat ttgtaaatgc tttaggggta gggatatcgg
      661 gtttcagtga agttaagttg gcaaatcaat tgatgcaatc atcgagtatg gcttctgatc
      721 ttggagagga tcctgtgatt tctatcccca aagagtttca tcaaatttga tggagcgctc
      781 atcttgattg taatgaactc tattgctgtt gtttttgttc aagttccggt tcgatttaac
      841 gggaaatata ttgtacttga taatgatggg ggagaggatg aattttttaa acaataaaat
      901 tgtaatgata accggcgcgt ccagcggcat tggacaggct tgcgcccgac tttttgctgc
      961 acaaggagca cgactcatct tggcggcacg acgggttgaa cgtttacatg aattatccag
     1021 cgagttaaaa catcataata atcaagaaca ttatattctt cctctggatg tctgtaagaa
     1081 agatttggtg agacaacaaa ttgaatcgtt gcctggtcaa tggaactcta ttgatgtgtt
     1141 aatcaataat gcgggtttgg ctttggatac cttgcctttg catcaaggaa tattggagca
     1201 ttgggacacc atgatagata ccaatattaa aggattgctt tatattagcc ggctaattct
     1261 cccagggatg ttagaaagga atagtgggca cgtcattaat atcggttcca tagcaggtca
     1321 tgagtgttat cctaatggaa atgtttactc ggccaccaag catgccgttc atgcgatatc
     1381 aaagtccatg cgattggata tgttaggaag tgcggttcga gtgactgaaa ttgcaccagg
     1441 tgctgttgag acagagttta gcgaggttcg ctggaaagac aaacagcgtg ctatagattt
     1501 ctacaaggat tttcaaccgc ttatggccga agatgttgct gatgctgctt tgtattgtgc
     1561 cacaagaccg ctgcatgtcg atattgagca aatgattatt atgccaacag ttcaggcttc
     1621 tgcaaatcat ttgtcaagga aaggcagata attatcaggt gaataattgt gcaaaaattt
     1681 gccaacaatg gggcggttag tctattattt aaaatatagg tgatgactga tcctggctga
     1741 accagtaagt agtgccagat taggaggtcg ttttggaggg tggtgtgtaa gcctacaaag
     1801 ggtaggaaac ctgaagctct acataaatct ccacagtgtc tttcagttgc ggctcagaaa
     1861 ccgggaagtc gttgcctgta cttaaactct tttctattct ctgtataaac tatttttttc
     1921 ttattaaaga atgatttaag tcgaacagat catagacaaa cccacaatcg ctttgttaaa
     1981 aatgttttta attcagtcat ttggtttaat taatctctct cattaaaaca ttttttaatg
     2041 ccccttatgg ttttgcgtaa atccagttaa agtcaatccc gatactttat gtttaaaata
     2101 aagagcaatt tgagcaatta ataatattat cttataatta aagaacaact attttgccag
     2161 gaagcaatta tgaacaagtt aattgttgcg gtcactgctg gtgcatcatt attgctgcta
     2221 actggatgtg cggttgatgg tgtggctgtt gatgatgttt cttacgattc aggttatagc
     2281 aatgactacg tttattcggt tggatattat agcaatcgtc cctattgggg taacaactac
     2341 tattcgtcac cttcttttgt aggtccttat ggatattggg gtggagatta cgatttctaa
     2401 tttccgtgag aaggcaagcc ttcaaatcaa atgaaggcta ttcctgctga taattactct
     2461 ttagcgcgag acacatattc gcctttgcgc gtgtctacct taatcagttc gccagtttga
     2521 acaaacaatg gtacacgaac aacggcacct gtttccaatg tagctggctt tccgcctccg
     2581 ccagaagtgt ctccttttaa tccaggatct gtttcggtga ttgccaaaat gacaaaattg
     2641 ggtggcgtga cctgtatagg ctcattattc catagagtaa gtacacaaac gtcctgttcc
     2701 tttaaccatt gtgctgcatc ggctaatacg ttttcagtca cggcatattg ttcaaacgta
     2761 tctggaacca taaaatgcca atactcccca tcgttatata gatactgcat ttctacgtca
     2821 gccacatcag cagatggtaa ggtgtcccca gatttaaagg tacgttccac aacgcgcccg
     2881 gtttttagat tgcgaatttt aatacgggtg aatgcttgac cttttccagg ttttacaaat
     2941 tcacagtcaa gaatgctgca cggagcatca tcaaccatta cttttaagcc gtttttaaat
     3001 tcatttgtgc tatagactgc catttctgct ccatagttga gattttgctg tattttcgct
     3061 aaagttcgca cagtttatca agtctgtaac tttaatgcga gatacctctt tgagttggca
     3121 aaaaattttg gcacaaggat ttacttcaac ggctgattta ttagatttcc tggagttgcc
     3181 gagatctgaa ggaaacctat tcgcggaaaa acaattccca agccgcattc ctttaggttt
     3241 tgctgagcga atgcaaaaag ggaatcccaa agatcccttg cttttacagg ttcttgcaaa
     3301 agaagatgag cttaccgtag cagatgatta tgttatcgat cctctgagtg aaagtaatac
     3361 ccttattaaa ggtttgttgc ataaatatca cggacgtgtt ttgttaactt taaccggtgt
     3421 ttgcgctgtg aattgtcgtt attgctttag aagacatttt ccttatcaag cgaataatcc
     3481 gggccgccgg ggatggaaag aggtgtgtgc ctacatcgca aatgatccca gtattactga
     3541 agttatttta agtggtggcg atccattgct cgctgccaat ttggttttgg aagaattact
     3601 ccaatctttg gaagaaattt ctcatgtaca tactttaaga attcatacaa gaattcctat
     3661 tgttttaccg gagcgtattg acaaaggatt acttgattta ttaacaaata cgcgatttaa
     3721 gaaagtgatt gttgttcatt gtaatcatcc gcaagagttg gatgagagtg tattgcgggc
     3781 gtgctctgat ttgaagaaag cgacatgtta tttactgaat caatctgttt tattagcagg
     3841 ggttaatgat gatgcagtga ttttatccaa gctaagtcat gctctttttg attacgggat
     3901 catgccttac tacttgcatt tgctcgataa agtaaagggt tcggcacatt tcgatatgcc
     3961 gcttcctagg gctcagtcaa tttatcatca attacagtcc cttgtgccag ggtatttatt
     4021 gcctcgcttg gcacgagaag agccgggaag atccagtaaa accttactga tttaaagagc
     4081 ttgatatgtc ctggctattt atggttttat tcaatagcca tgtttccact gttatttttc
     4141 tgactaaatc gatttgtcct cttcctcata aagtgcaatc agcttttcct gagcacagcc
     4201 gccgttttgc atgaggtgct tgtaagtccc atcactttgc atttcccaaa cattgttatt
     4261 atctttaaga tagtttttaa tgatttcgtt ttttattctt tttttacatc cctcatcata
     4321 aatggggaac atgatttcaa tacgattgta taaatttctt tccatcagat cggcactgga
     4381 gcaaaaatag tgttcttctt cgttgatgcg aaaataaaac actctgtgat gttcgaggaa
     4441 tctgccaata aaagacaaga ctctgatgtt ctcagacaca ccaggcaatc ctggttttaa
     4501 acaacatacc ccacgtacaa gaagattaat tttaacgcct gcctgtgatg ccttatataa
     4561 tgcttgaatc atcactttat cagttaatcc attcactttc agaatgattt cagtatcaat
     4621 gccccccgta gcagcagtac tacattgttc gatgaattgt aaaagagttt tttgtaaggt
     4681 aaatggcgaa tggctcaagg cttttaactt aacaaccttt ccaagaccgg ttaattgttg
     4741 aaaaatgagc tgggcgtcag aagtaatagt gggttcactg gttaataatc ccaaatcagt
     4801 gtatctcttg gctgtttgtt catgatagtt tcctgtgctt aagtgcacat atcgttttaa
     4861 tttaccatgc gttcttcgaa ctaccagcgt cattttcgcg tgagttttat agcctactac
     4921 tccgtagagt accaggatcc ccgcagcatg gagtttgtta gccaatttta agttggattc
     4981 ctcgtcaaaa cgggctctta gctcgatgac agcggttact tctttccctg aatgggcggc
     5041 gttaaccagc gcatcgacca tcactgattc agagtgggtt ctgtaaagcg tttgattaat
     5101 tgccagtaca ttcgggtctg aagcagcttg cctgacaaaa tcaatgacta aatcaaatgc
     5161 ttgataggga tgatgtaata aaatgtcttg ttcgtcgaga atattaaata aattacgttg
     5221 tgatttggag aacttgggat actgtggggt aaaagcggga taattcagat caggtctatc
     5281 gatgctgtta atagcactca tatatcgttg taaattaacg ggcccatcac aataataggt
     5341 gtcttcatgt cgcaaatgat atttctgcag gaggaagtca acaatttttt cagggcattt
     5401 gttttcaatt tccaatctca cgacatggcc ataatgacgg gaaaatatct ctctttgcac
     5461 cgctttagcc aaatcgtcaa tttcttcttc tcttaaaaat aaatcactat ttcgcgtcaa
     5521 ccgaaatgaa taacatccgc ttatttccat acctggaaat aagctattaa tatgcgtttt
     5581 aataatggaa gataaataaa caaagtaatg ggcatctcca caaagctcag aaggcaaatg
     5641 tatcatgcgt ggaatcgagc ggggagcatg tactacggcg taattgatat ttcgatcaaa
     5701 agcatctttc ccactcaatg aaatgataaa atttaaactt ttattgataa gccttggaag
     5761 aggatgggcc aaatccagag caatagggct tataacaggt aaaatttcgt gtttaaagta
     5821 atgctttgcc cataaatgaa tgtcatcagt ccattgatct gtctcaagaa aatggatatt
     5881 ttctttgcgc aatgccggaa gtaattgttt gttaaaaata ttgtaaagct gatcgattaa
     5941 agcatgagct ttatggctta tttggctgaa tgcttcatca gcccgtaatc catctatggt
     6001 tagcttgcct gacgacatgg caattttttc tttcaacccg gccacccgaa tttcaaaaaa
     6061 ttcatcaaga ttactgctgc agatactgag aaatctcatt ctttccaata agggtacgcg
     6121 ttcatcatta gcaagcatga gtactctctg attaaacgca acagcagaaa attctctatt
     6181 aatataataa tctgggttat ccagagtagt atccatgagg aactccaggt attttcattt
     6241 catgttcaca ggcaatttag tcaccatgca aagcccttac atctggaggg attttttgcc
     6301 aggggtaacc ttttaccgct ttggaataac caatttgata taactgattc attctttgag
     6361 tatcaaacaa ttcctccgag ttcgaatcaa actcatttgg aacattggcc aagttaaaat
     6421 caacgccctc tataagagca caaagataga taaaaataac atcagcagca ccctgggatg
     6481 ccgtgacact ttctatcgct cgcttgccaa ttaccaataa cttcggcttg attactttgt
     6541 aatggaattg attctggtta ttgcgtatta cataaatttt aacgtttggt ttttgtttta
     6601 atttgaaatg ttttttcgcg gttttgatat cagaaccaag acgacttaaa aagaccgcaa
     6661 acgttgttga gccatcgacg tgcatttctg tgtagggttt tccgttggcc atgacatcaa
     6721 aataaacagg cggcatgacc acgggaatgc tggcagaagc aagtagaatt tttcggaata
     6781 actgaactct ctcggggttt ttgcttgccg caatgcgggt catatcccat accaccagct
     6841 ttcgagagta aagattggat gtccctacat aaagccgtcg tccttgataa tagcctttgg
     6901 caatgtcatc aagcaatttt tcatcgacat ggcgagcaat caggtcttgt aatggtttgg
     6961 ttcctttaag ggcgctacct cctgttaaca gagtccataa tacaggttta ttactcagta
     7021 catccttcgt ggtaatggtt gtatacactt tttttaatgt agcatcatac ttggggccag
     7081 caaaagctaa aggtgctatc aatgccccag tacttatacc tgtcacggtt gtaaatgttg
     7141 gccgagaacc ggattttgtc cacccattca gtaatccagc accaaaagca ccataatcgc
     7201 caccacctga aataatcagc aagctgaaag agcctttttg atggaacatg gtatcagggt
     7261 aagctttagc cagttgtttt acacgttcaa gagattgctt tttaattatt gggtcatcac
     7321 tgcgggtgaa ttcagtgcgt acctctttaa atccgggcac taaggcctta tctgataatt
     7381 gcgctgggag aggattattt tttgctgtat tgcaagcggt ttctaaacaa agcaaaaata
     7441 caacaaaaaa aatccttttc ataattttcc tttaaattaa tgttttcctg aattttattc
     7501 aaaaaatgga ttaaaagcga tagtgcttgt tttcatatta acgcaccaca aaaaaacata
     7561 cggctataaa tgctaaaaaa ccataaaagg gtgatagatt ccaaaaggcc aaggcaagaa
     7621 aaaacaaaat acagcaaata gccataggaa tagagtaata gacgtcgata acaccttgaa
     7681 ctactgaaaa tgtagcagcc gctaacaagg ccaaagctcc gtattggact gagatcataa
     7741 ttttgcgtgt tattggaccg ttatgagaat taagccactg atatcccaca agagccataa
     7801 ttaatcctgg taaattcagg cagatgatcg caagaattaa accggttatt ccggccgctt
     7861 tatagcctat tagtgcggcc agttttattc ccgttaatcc cgggaataaa aaacttgaac
     7921 ctaacatacc tacaaattct tcttttccta tccagtgcct gtacgtgact gcctcatatt
     7981 ccaataattt taacatggaa ttgccgccgc ctaaggatat caggccaatt ttcccgaaac
     8041 tgtaaatgat ttcaatgaaa gtttttatca tatttttaaa gtctttttta agaaggtaac
     8101 gtattcaaaa gaaggttggg ttccgaataa aaggacgctg atggtttcta ccagttttaa
     8161 tgatctcccc tcaatacagg cactgaaata cactttatcc ttgacttcaa gacgagcaag
     8221 ctcaagcttg acagttggcg ttgtagggaa tttaaaaaca aaggcttctt ttttaacaaa
     8281 aacaacgatt cctttttctt ttattttttt caatagttct tgtcgattta actgcccagc
     8341 atcgccatgg caagagagcg tttgtgggtt ttcaagatga actttaggtc caaaacctag
     8401 tgcagagcca gattgcttaa taacaggttt atacaataat tcattacgcc tttgctttat
     8461 attatgatca gagtcaggca gtaaataata atagtcttct ttatgttttt tctgatagtt
     8521 ggctagatca agcaacgaac tgtcaatagt atttaaacgg atgatttgat ctgcaggcca
     8581 aaaatagcgc ttttcccatt ttagattgtc ttgagcatca tgatctaaag tggtaagtgg
     8641 tgttgtttgt tcagaggaaa attcaaagtt ccaaaccagt ctttgcatta ttagtatttc
     8701 attttctgtt ttgcttacgc tagatgattt gatttgtttt ggcaaggtta acttaaatgt
     8761 ttcttataat ttttaaagga ttattgctcg ctaaataaag cgtaaaaagt atatactggt
     8821 tattctataa acctcttaat aaaatagcaa aggaaggtat tttatggcta ttgtgttctt
     8881 tttaatttat ttggctttta ctctggtagt actgtccaga agaattaatc cattgatttg
     8941 ggaaattgga agcataatct accttattcc cgcaacattt tattttgggt tcccctggat
     9001 tattggttta ttcatttggt tctttattat agctgccgtg ctggtagtta gagtggagcc
     9061 attacgcttt ttcatcgggg attatttata taaaaccgca ggaaaatcgg tgcccaagct
     9121 ctctaaaacg gaagaagaag ctttaaacgc gggggatacc tggctggaac aagatatttt
     9181 tacaggaaaa cctgattggc aaagattatc agcagtttct acagagctca cagcagaaga
     9241 acaagctttt ttggataatg aaactcatac cttgtgcagt atgttggatg aatgggaaat
     9301 tagccaggaa tatgatttat ctcccaaagt ttggaattac attaaagaga aaggattttt
     9361 agggttagtg ataccaaagg aattcggtgg caaagggttc tcagctcgag cccattccga
     9421 tattgtaatg aaaattgcaa ctcgctcaag agtagcggca gtgacggtaa tggttcctaa
     9481 ttccctgggc ccgggtgagt taataaatta ttatggaact gatgagcaaa aggcatatta
     9541 tcttccgcgt cttgccaaag gtgttgagat cccctgtttt gcattgacag agccaggggc
     9601 tggaagtgat gcgacctcca tccagtcaga agccattgta gttaaaaaga agatagatgg
     9661 aaaatcagta cttggcttaa atatcacctt ggataaacgg tggattactt tagctcctgt
     9721 tgcgacttta attggattgg ctgtgaactt gaaagatccg gatgggcttt tgaagggtga
     9781 aggcgaggaa ggcataactt gcttgctgat tccaagagat actgaaaatc ttgaaattgg
     9841 caataggcat ttaccagctg atcagccctt tatgaatgga actttacgtg gcaaagatat
     9901 ttttgtgcct attgatacca tcattggtgg tcaaaagaaa gcgggatccg gttggcaaat
     9961 gctggttgag tgcttatcta tagggcgctc aatatctctg ccagcgcttg gagcaggttc
    10021 ttcttctatt tcttatttaa caacaggtgc ttttgccaga attcgtcgcc aatttaatgt
    10081 agaaatcgct cagtttgaag gagtagagga aaaacttgca gaaatagcgg gtttgaatta
    10141 tttgatcaac tctacccggt tgttgacggt tgctgctgtg aatgagcata agaaaccctc
    10201 tgtagcttca gcgattacca aatattttaa tacggaattg gcacgaatca ctgtgaacgc
    10261 ttctatggat gttcatgctg gacgagccat tgttgttggg ccacgcaatt acttaaatag
    10321 tttatatcaa agtgttccta tttctattac ggtagaaggg gctaatatta tgtctcgcaa
    10381 tttattgatt tttgggcagg gatctatggc ctgtcatccg tttattcgtg atgaattcta
    10441 cgccatctcc agtgagaata aagaggcatt tagagagata atttggaagc atatccatta
    10501 ttttatgcag aattttgcta aaacagtctg ttcagcatgg acaagtggtt tgtttattgc
    10561 ggttcccgaa aattcaatga aaagagagta taagaaactg gcgcgtttga gttatgccta
    10621 ctcttggctt gctgatctgt ctttaattta tttgggaggt gatttaaagc gcaaggagcg
    10681 attgtctgca agattggccg atggcatgtc ttatctctat atggcaatgg cggctttacg
    10741 aaatgtgcaa ttaaatcaag accatcctga tgagcaattg catgccagat gggcggtaag
    10801 ctattgtttt taccacgcgc aaaaatccat gattgcactt tgtcacaatt tcccttccaa
    10861 atttttaggt tttattgccc gagtagttgc gtttccattt gggcaaacca tgcgttatcc
    10921 ttcagacaaa ctagatcatc agttggctcg tttgatgacg ggcaacaatc attatcgtga
    10981 ccgtttgaaa aaatcggtct ttctcagtgg tgattcgaag cagcctgtgg atcgtatgga
    11041 acatgcttta cagcttatta ttgaagcgga tggattaagc aaaaaagtga gtgatttaaa
    11101 gcgctataag tggagtaagt tgaaagggaa gttaaaagaa aaagtagagt ctggccagtt
    11161 gtcacaacag gaaatggaag agttgcttgc tgttgaagac gcacgttggg atgccatttt
    11221 agtggatgaa tttaccttcg actccatgaa gaagaagaca ttgcattccg tgatagaaaa
    11281 aatgaaatct cccttggcgt gatttttaca gacttttaac atcgatttgc caaccagcta
    11341 tcaatccaaa tgaggctggt ttgctataat taaactatat ttcctggacc aaatatacat
    11401 tgttgagagt aagttatgcc aaaatacaca ctgcccacaa gagatgcctt attaaaagca
    11461 atgcaagtag gtgaaacatc cattgaagcc gctgaatata tggcaacaag gtttgagcag
    11521 attttaacgc aagcgaagtt attaccagaa tgtaatgata tgctggaaaa aatacaggaa
    11581 tatgctcaat ttgttaaatt caaattatta tcctctgctc aagtttggtc tggccaagaa
    11641 agaccaattt cggattacca aaatatgcag gaaaataaag ctgaattttt ggcaagtcat
    11701 ttgaaagagt tgccaagtgg attaaaactc gaaattgcta ttggggacga tgccaaaata
    11761 ttgcgaggat tttcttccaa tggaaaaatg gtagaaggag aacagctgaa aaccatggat
    11821 ggattattag aaggatggct tgccaagaat aatcttgcta tttcaggagg tgctgttgtt
    11881 caaagaaaca gtactggcaa ccaaactagc gtagacccag aggaaatcag aaaattaata
    11941 aacgatagtg aaaagggagt cgccaaatat tttgcagata aaggcgtgag catggaggtg
    12001 gtacaacgaa cctaccaaga gcccaaagca ttggaaacca agcgagaaga aatcaggcaa
    12061 gaaattgaat ccggtgctga agccccaacc actcaatcca tcagatagga atatactggt
    12121 tcccctaaaa accaagattg gtttttaggg gaattgattt gctgcatgca gtctatcgaa
    12181 agagcctatg tcataccaaa ttcctgagtg aacactgcct gtggcactat tttgttccac
    12241 atattttctg agtaaagggg taacagaata ccttccttgt ctgcagttgg caaaaacctt
    12301 tgggttatag caggcaatac ctgaaaaagt gtaagcctga tttgtattgg ttaagtggga
    12361 tccattgatt aagccaaaat cgccatgatg aagcagagaa ggattttttg gtattaaaat
    12421 aagatgaaat gtatcaatat ttttgagttg gagcttggta aaatcaaaat cagtgtaaat
    12481 gtcggcattt actgttaaaa atggcccctc gcccagcaaa ggcaaggcgt tcacaatacc
    12541 accccctgtt tcaaggcctc ctggcggttc tggagaatag ataacattca agccccattg
    12601 ttttccattt cctatatatt ggcgaatttg tccgcccagg taggcatgat tgattatcaa
    12661 tcggtcaaag ccggcattgg ccaggttaat gatatggtgt tcaattaacg gcttgtttct
    12721 taccgtacaa agtgctttag gaattttgtc ggttaaaggg cgtaatcgtt ctccacgtcc
    12781 tgctgcaaga atcatagcgg ttttcatggt aaataaactc gtttttgcag aaaattaaaa
    12841 aatgggtgta attcctcata ggtttctgcg cattctaatg catattttaa cgtgagaggc
    12901 aagtccccaa gatatcctgc tttattatca cgcaaatata aacggcaaaa aacacctaac
    12961 acttttaaat gcctttgtaa cccacataga tcaaaggccc gaataaaatc ggttagagaa
    13021 taaatgttgg tgtgggggga tttttcatga aaataggtta tccattctaa aaccttttct
    13081 ctcggccatg aaatataaca atctttgagt aaagaaacta aatcataggt aacaggacct
    13141 agcatagcat cctgaaaatc aatagttgct aaacagctat ccttattttt gactagcatc
    13201 agatttcttg aatggtagtc cctgtgaatg aaaactcttg gttgagtcgc tacttcactt
    13261 gcgatccact cgatggtatg ctgaaaaagt tgtaattcgt cttgttttaa atcaagagaa
    13321 aggtaagctt tcaaaaacca ttccaaacat aaattcattt ctttaagcat gaaacttttg
    13381 tcaaataagg gaagttcagg atcgtcagtg ggacattttt gaatttgtaa tagcaaattc
    13441 attgcctggt tataataatt atccactgtt tcatgattca gcttatttaa taataattga
    13501 tcccctaaat cgcttagtaa taggaaacct tctcttttat ttattgccag aatatcgggg
    13561 actggtattt taattttatc aagaacgtta gcgatatgga ggaaagattc aatattttcc
    13621 ttttcaggcg gagcgtccat aactacttga gttaatccat tatattgaat gcgaaaatac
    13681 cttctaaaac ttgcatctcc tgctaaagga aggaggacaa agtctttttg ttgaatcgtt
    13741 tgagctaacc actctttcag tgcgttttct ctttcatgca tggtatactt cccgttttta
    13801 tttacagagt gcggttgata tactcccttc ttaataaaga gtaatttcat tcgcgcacaa
    13861 ataataaggt attttgttag ctgtgaacat gaggttttat aaaaagctaa ttatatccgg
    13921 ttttatttca tttatcgcct tattgcgagg tttactctat tgccatttaa aaaggttatt
    13981 taaaaaatgg cctgggtctt tattttcagt gaaatgggtc ttaatggcca tagggataac
    14041 tgcttgtgtt ttatctttga taaattatca aggcttagcc tactccgctg ccttgataaa
    14101 tgaacctatt caagcttgcg taattgcgcg agaagtcgat ttaacagacg atataagaac
    14161 taaatttgcc caatgtttgg gctggcaagc tgatcaaagt tcccctgtgt gcctcggttt
    14221 ttacaaaccc attacggtga caccgttagc tagtcctgat gaagtacgta ttctggcaga
    14281 tacggcttcc ttctaccgta ctcagagatc cactttatct gggcatgtag aaatgcagca
    14341 gggccaaaga gtagttaacg ctcaaacagc ctatgtgtac agggatccta aaacgaatga
    14401 agtaacaaaa attgaatttt tgaatcatgt acgttattta gaacctgata gaatgatgat
    14461 cgctagaaaa gcagttgtgt atcctcaaga taaatcgggt gaagtggaag atgttcttta
    14521 tcgatttaat accaatagaa gcaatgcatt gctgcctgcc tggggtaggg caagtttaat
    14581 caagcgtttt gcaaatcagg actacttttt gaaagaggca acttacacga cttgcgcgcc
    14641 tcaagataag gcttgggcta tcgaggccga atccatcagt atagacaacg aaaaaggaaa
    14701 gggcattgct cgcaatgcca aattacgtat ccacgaatgg ccggtgcttt atacccctta
    14761 tttaagtttt cctaccaata gagatagaaa atcggggttt ctgatgccca tagtgggtta
    14821 ttccaatgta gggggagctg atttgggcat cccttattat tggaatatgg cgccaaatta
    14881 tgacatgact ctcgttcctc acctttatac aaaacgcggc ttgatgttgg gaggacaatt
    14941 tagatatttg acttcaaaga gcactggaac atttaatgga aattttttac caaaagataa
    15001 agcctttggc aggtttttac aggacaatga ggtagagttc cctcagattc gaggactttc
    15061 cacgaatcgt tgggaagtaa attttgtcga ttcaacccaa tttctctctg atcttcaatt
    15121 aaatgttaat tttcaacagg tttctgatga ttattacttg caagatttca gtacaaattt
    15181 ggcatcggta actcaaagac aattattgcg ccaggctgat ttaacctaca ctaccgaaaa
    15241 ctggactttt agaggtatgg gacaaagtta tcaaacgtta catccaatta acgaaatccc
    15301 ggtttcccct gtttatgaac ggttaccgca acttatggca cgaggttatt atgatgattt
    15361 accttttaat gcccaattca acattcttgg tcaatacgac cagtttcact ggcctaatga
    15421 ctcttggaat atagcactta ataatatgcc tcagggaccc cggtttcatc tgaatcccat
    15481 tttatcagtg ccaatgatga agccttgggg atatgttact ccctcagtgc aatttgtgga
    15541 aaattattac gatatttcaa gaaattatac ttggggcaca tctcgagcta actataacct
    15601 gacaatacct cgctatagcc ttgatggcgg attgtatttc gaacgagatt tgcatcttaa
    15661 gggtacgtat tacatacaaa ctttagaacc caggttattc tatttaagag taccttatta
    15721 taaccaaaca cttattccag tttatgattc aggatttatg atttttaatg tcgatcaatt
    15781 gtttagaacc aatcgctttt cagggtttga tcgaattgga gatgccaatc agctaagtta
    15841 tgcccttact acaaggtggt tagaagatga atcaggtgct gagaaagcga atttttctat
    15901 tggtcaaatc aaatatttca gtgagagaag agtacaactt tgtcaaagcc ctaccgggtt
    15961 ttgtaccgat aatccagata cctttggaaa cttatcttct acttttggaa catcgcctgt
    16021 tgcgtcaagg gcggtatata aatttaatcc agcttgggga ataacagggg attacatttg
    16081 ggatccggca acaagggcta caaataatgc ggatttgaat ttgcactatc agccagctcg
    16141 taatgctatc atcaatggcg ggtatagtta tttggttaat ggtgacgtta ctcaagtaag
    16201 aaataatgac actgaaaata atgccttaca tcaagctatt ttatccgctg cctggccgct
    16261 tagtgaaaaa tggagtggta ttggagccta tagttataat atcagtaaaa attacagtat
    16321 gatgtcattt cttggcgttc aatatgatag ttgttgctgg gctatgcgta ttttgggagg
    16381 gcgaactttt agaagtttaa atgaagaatt tgagcctcag tacaataaca acatttattt
    16441 gcaaatatta ctaaaaggct taggatcagt agcaaacagt gatccaagtg gtatattgaa
    16501 tacctatatc ccaggatact atgacccttt ccgacgccgt taaaatccaa agataaatga
    16561 cgcaataaaa gcgagtcagg tttaggtgtt taggttagta caggttgaaa atattttaat
    16621 ctttttttta tgagttatgg tgtaaattaa gcaaacttgc ttgtattttg acaaagaata
    16681 acataaattg cccgaaaaat gtaaatgagg attatggcat gtttaaaaga atagcattgg
    16741 tatgcgcttt gttttcagga atatgtttcg ctgaaggaaa gcagctgtta gataaagtgg
    16801 ttgctatagt aaatgataat gtcattactt ctagcgaatt gaacgctcaa gtcgagctat
    16861 ctaaaaagca gattatcgct caaaatatgc aaatgccaga tgagtctgta ttacgtaagc
    16921 aagtattgca acatttaata gacgtagatc tggaaatgca aatggcaaaa caaaatggta
    16981 ttactattga aaacgctgaa attgatgaag cgatagaaaa aatagctgct tcaaatcatc
    17041 taaatttatc gcaaatgcgc gatgaaatta caaagcaagg aattagctgg caagaataca
    17101 gacaaaatat ccgtaaagaa atgctgattt ccagggttca gcaaaaggca gtaggtaaag
    17161 atatcattgt tactaatgag caggtggaac aatacttaaa aactgcaggc cgcatagaaa
    17221 attctaactt gacgtatcat ttaaaaaata ttgttatccc tttaagcgaa gaaccaacga
    17281 ccaaacagct tcaaagagct aaaatcgagg cagaaaactt gttaaataaa attaaaaaag
    17341 gtgaagactt tagccgtttg gcaatagaag aatcaagtgg agagtttgca ctggaaggtg
    17401 gtgatttggg tgagcgtcat ttggcggagc tgcctgaggt atttgccaag gaagtagtac
    17461 atatgaaagt aggtcaggtg gccggcccga taagagcagg aaatggattt catttgatca
    17521 aattagtagc tgttggcggc gaaaatcagc gtcatgtgat cactcaaacc catgtcagac
    17581 atatactgct taagcccgat gccagcatgg tgccttctga agcaattaag caagtcaata
    17641 acatttaccg gcaaatacag tcaggcaaag attttgctct tatggctaaa caatattcac
    17701 tcgatgctgc cagcgcagtg aaaggcggtg atttaggatg ggttaatcct ggtgaattag
    17761 ttccagagtt tgaaaaaacc atgaacagtt tgccattaca taaagtcagt aaaccggtaa
    17821 aaacccagta cgggtggcat ctcattgaag tgattgcacg cagacagaag gatgattcag
    17881 aagcttttaa gaagcaacag gtcaggcaat ttctacaaca gcgtaagttt gtagaggcag
    17941 tgcaaaactg gcaacaacat ttgcgttctc aagcttatat taatatagtt gataaggatt
    18001 tagcatgaac ccactgctta ttagtagtgg agagcctgct ggaattggcc cagacttatg
    18061 cctggctttg gcagaaaccg atttgcccgt agtgatattg ggagatctgt ctttattaga
    18121 ggcaagagcc agtgagttaa atctcagtat taaattttta gagtacggcc ctcatcaaag
    18181 cttcgaaaaa aaagcaggat atttaactgt atggcctgtt ccatgcgctg caccggtcat
    18241 tagtggtgaa ttaaatccgc aaaatgcggc ttatgttatg gagcttttga ctttaggggc
    18301 ttcgctatgc tccaaaggtg agttttccgc attagtgaca gcgcctgttc ataaggcaaa
    18361 cattaatgca gctggtatta cgtttactgg ccacacggag ttttttgctg atttctttga
    18421 ggtggaaaca gtggtgatga tgttggcctg ttctcaaatg aaagtggcat tagtcacaac
    18481 gcatcttcct ctgcgtatgg tgcctgatgc cattaactct ttgcttatca taaaggtaat
    18541 tcaacaatta catcattcat taaaacatga ttttggaatt caaagtccaa gaatcaacgt
    18601 ggctggtctg aatccgcatg ctggagaatc aggatattta ggtcgagaag aaattgaaat
    18661 tattactcca gccttaaata ctctaaaaaa ccaggggatt gatgtgctag ggccgttacc
    18721 agcagatacg atgtttataa caaacaatat caatcactgt gatgcttatg tggcaatgta
    18781 ccatgatcaa ggactgccag tcttaaagta tgctggattt aatgaagcag taaatataac
    18841 tctcggattg ccgataattc gtacttctgt agatcatgga acagcattag agttagctgg
    18901 taagaacaaa gctaaccctg ggagtatgct tgcggcggtt aagatggcga aagatatggc
    18961 tttaacaagg ataaaatgaa atgtctgtga ttagcctgat agcagccata gatgaaacag
    19021 gaggattggg tattaacaac caattactct gctatttgcc agccgacttg cagcacttta
    19081 aatcgattac catgggtaag cctattatca tgggcagaaa aacttatgaa tcgataggca
    19141 aacctcttcc aggcagacta aatattgtga ttagtcaatc gattcagtca ataccgggtg
    19201 ttgttgttgt cgattctttg ggccaggcca taaaagaaac cactggcgca tcagaggtaa
    19261 tgatcattgg aggggccaag atctattctc aagccatatc aatggctaat cgcttatata
    19321 tcactcgaat acaccatcaa tttacggccg atgtattttt tccaaaaatc aatgaattgg
    19381 tatggagctg ccgaagcaag gagtatcgac agcatgatga caaaaatcaa tacgatatga
    19441 cattttgcat atatgaaaga atatcaattt gatagctatc aataatgata atgtctgttt
    19501 tgtgagggat gtctgtgcac tggtggctga gtccttacaa ttatttcagt tggcctgctc
    19561 agtattctat aattgtccgt tataatttcc cgaccaccat aagaaatatt acaatgtgag
    19621 taaccaggcc atgttttccc aggctgaata cttccattga agttactttg acagacaaat
    19681 aaaggattac cattggtatc tttacccatt aacaatgcat gctctacgtt gggttcccag
    19741 ctgtatcgcc caaattcatt ttgattcggt atggtaaact ggctaagaac gtattcttta
    19801 ccaccataag gtacgttgca tcgaccatat cctgcccatg tttttccagg ctgtatactg
    19861 ttaaacagtt ttgccttaca cagatacaat gcattgccat tcgtgtcagt tccagttcgt
    19921 aacgcattag ctaacggtga aacaggatgt tgatggtaat gagtattagc ccaggcagga
    19981 ttaagaaaga atatcatcaa taaaaggtat acaagtttgg acatatcaac tctctaaaaa
    20041 tttaaagagc cgcaagcata acgcaagaaa aaaatatgcg caagatgaat acttaaagat
    20101 cgatatggtt tatctattgt aacccaaaga aaaacaatta aaaaaaatta aaaaagtatt
    20161 tgacagtgtt gttgaaatga gtagaatacg ccctacgcca cgagggcggg ttcattaaga
    20221 agagagaaga caaactgtgt gggcactttg agatactttg agtgctttag agagataaag
    20281 aaatgagcta gcgagagcta gtgccggaat tgaactgaag agtttgatcc tggctcagat
    20341 tgaacgctgg cggcatgctt aacacatgca agtcgaacgg cagcattgtc tagcttgcta
    20401 gacagatggc gagtggcgaa cgggtgagta acgcgtagga atatgccttg aagaggggga
    20461 caacttgggg aaactcaagc taataccgca taatgtctga ggacgaaagc tggggacctt
    20521 cgggcctggc gctttaagat tagcctgcgt ccgattagct agttggtggg gtaagggcct
    20581 accaaggcga cgatcggtag ctggtctgag aggatgacca gccacactgg aactgagaca
    20641 cggtccagac tcctacggga ggcagcagtg gggaatattg gacaatgggg gcaaccctga
    20701 tccagcaatg ccgcgtgtgt gaagaaggcc tgagggttgt aaagcacttt cagtggggag
    20761 gagggttgat aggttaagag ctaattaact ggacgttacc cacagaagaa gcaccggcta
    20821 actccgtgcc agcag
//
LOCUS       DATNSN010000039        20724 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.039, whole genome shotgun sequence.
ACCESSION   DATNSN010000039 DATNSN010000000
VERSION     DATNSN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.039"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            153..1016
                     /locus_tag="VAH76_001227"
     CDS             153..1016
                     /locus_tag="VAH76_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEO1385869.1"
                     /translation="MTDIAVIINNRAKNAHRMEDYLSELRAHDIDYQLYKADPQQLEK
                     NIQHCCSKYPLLLIGGGDGTVRSAAQWCSNTSVILGVLPLGTMNHFSKELNLPSTTEE
                     LIAAIVEKTTTTIDVAEVNGHIFINNSSIGFYSKLAKNRDYYTNFYNKWLTYIPSFIQ
                     ALSYHPSYALLIKNKELDISIRTSFFMVSNNCYTYEFPLKFTRESFQNEQLGIYYYKR
                     SKLRLFKFLIDFLTDKEHFEIIQTRLPLEVDVLHKNKITISLDGDTLAIAPPLYYKIL
                     PKSLKLLTKKI"
     gene            1013..1825
                     /locus_tag="VAH76_001228"
     CDS             1013..1825
                     /locus_tag="VAH76_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO1385870.1"
                     /translation="MKIIHISDLHFGKHQPAVLNAFLQETALDKPDIILISGDLTQRG
                     LSYQYRELCSFINQLPAQTLTVPGNHDIPFYNVPARFIFPFRHYKRYINSDVTTTFEN
                     DCVRILGVNSVNPLQLKDGKLSHETLNRIERYFKPDDEKLNLLFFHHNFDYMEGLHKP
                     LANAQTLLSFLKQSTIHLVCTGHLHYAHLSLIAKNNGYSCLALHAGSLLCPRSKDNLN
                     SYYVIEANQKKCRITWRVFDQEHFTARSIYFIDFNKKNASFEHINPVKDDTC"
     gene            complement(2007..4241)
                     /locus_tag="VAH76_001229"
     CDS             complement(2007..4241)
                     /locus_tag="VAH76_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385871.1"
                     /translation="MKPNLFYFVLTQILAESPNYKLLDEITPVHDITLKDKKYYRLEL
                     PLTNIAAEGFRLHDAHISLYEKTDPHNPNLGPSHFTAIFQDPNGQTYRMHLFLNRFDA
                     LACPATWELLDDDEQYVKISPPENLEYFIHSVWQLGLPCLQLLRKKQKEIEAKLLADY
                     HRLNEETTLLSKDLDKNRAVYLEKLDELIKTTKSLSQISESNHWLREAIYLGKTHKYV
                     SSMPEPILEPVNKGDEEQKKDDQAEVAALSPKQGATKPKISRGNIGLAQSTLFSKSAK
                     AKRPVDVKMERDISKIKKLFAQLLKTEDKKIKAVLLIDLHWKIKDINLETEELLIKEQ
                     TQALNEIEGRANKEAKSLLERALFAGEFEYAQTLSPYYPLINNDLMVLALTQRKADLL
                     SFLVTKVGLPINSYPIKTKVKTYSNAVEYCFSEHSESLVDCFSVLIKNGASLMQPVGL
                     HKLPLAHLLLSEIPRHPLYAALEKNKSSTINNKQFYAHLINALKSCLLSDDIEGDQKI
                     ALEESIARYEHLKTNVKNSSSLLSPQNQALADEISDITKKLLSDAVAEAIESDEEISR
                     EQAMADKEYKELVRKVKAFYHKTGKNFAFQTLINSANQELKKELLKIDFNIDISFSEL
                     KKSILENLSNERLMLSYCSELIDVQTTIMQISQGASRKNKNLKKLNARHAELIELLTS
                     LSQDTPQSMIKEANEFQDSFSQLQDLLNGLNELEGSFSMLAGVLNQFIGGSTSESESF
                     GVKL"
     gene            complement(4770..6059)
                     /locus_tag="VAH76_001230"
     CDS             complement(4770..6059)
                     /locus_tag="VAH76_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEO1385872.1"
                     /translation="MSFYIQLASLSIIILYVLLLFRNVDPLVATVVCVGLGFLWNIST
                     PIDIGNAMADALGSFMALVGFIIMLGRGLGEVLTHTQVSHTLVHQIVYGVGVNTQRRA
                     KIGIVLSSFIIVGLLGTLAGGLAILAPSLRPIAGSVGLSRPSLAVLMQASAEEALILG
                     PFAPPVVTLLGVTGLSYGTVFLYASLPIAVVTLITTWFMASRLQKLYINEPCEDENST
                     EPFTPNRQQKRATLIFLISFLFCVVYGLFTQAKTSYVIFVMLFLALITGLSCRLGLKQ
                     IFNLLFEGMQKSLHLFFLFILFDPFMALIHQAGGFNALTELLTPLINLGGQPVLSILI
                     GFTGAFGMPGAAEATIKMLHQLFSPSVLQMQLPLITFALCMLFATRVTNYAYPGANMF
                     AAMGFAGSENVKAMIQNGLVVTGVQVVFLIVYSLFIP"
     gene            complement(6059..7162)
                     /locus_tag="VAH76_001231"
     CDS             complement(6059..7162)
                     /locus_tag="VAH76_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEO1385873.1"
                     /translation="MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIG
                     YGSAASTPAITGDSKESIIAAIKNALGPPLIGRDISAFNLLLEMNDRAIGGNSSAKAA
                     IDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLK
                     IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPVVE
                     QPVNARNLPDLKTITDQVDCAIIADEACFSPEDALNIAKINACDGVNIKLMKSGGIEK
                     AQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYADLDPIFLIRENYILG
                     GAQSLSNKIILADKPGLGIEGITQGLNLIGVIR"
     gene            7633..8445
                     /locus_tag="VAH76_001232"
     CDS             7633..8445
                     /locus_tag="VAH76_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEO1385874.1"
                     /translation="MYELNDTPYFIYSNLIQEYCSPLFNLTPIKGFTFCRTYGDKTAF
                     LLTSEYQFHIECIQHEKKISKLNMLRTPEYTQAGYFLNSPALDGKYSPFYESTAQKYG
                     YGFSFTIVRKNENHCDYFHFIGNRHQKDMNNYYLHNKWLLDKFCDYFLERAAKVLAYS
                     EKENRFTLTNTAEHPFPLKIHSSALDQFLKDYHFEQFGLKPVCLPKRQIECAQLLVRG
                     KTAEEIAIILNLSKRTVEHYINILKRKLNAHNKGLLITKLLHQYPDMFESIL"
     gene            complement(8475..8879)
                     /locus_tag="VAH76_001233"
     CDS             complement(8475..8879)
                     /locus_tag="VAH76_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385875.1"
                     /translation="MTSFFLRKNITKSAKVLLEKEAEKIFSRNGQGLTFLEKGYKVMT
                     YTKHPVQNDDLILAYSPNVIYSNERMNKIREVMRRRQCGFALFRVKEDTFCQVKQLKN
                     YPEDLEARDTSQFFVTSETLEPVKFLESSPPP"
     gene            9065..9832
                     /locus_tag="VAH76_001234"
     CDS             9065..9832
                     /locus_tag="VAH76_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385876.1"
                     /translation="MFFKTCEFQRNKSSDTVYPRIDIRQAIYKESISLTPEMAISMQK
                     KGGYQTDNDWKKMLMGDVTQNLLIQSVIGKKSLICAPPNINSMSLEDFHLWTKQPLPA
                     GTSQYLALPNAFADNLIHGYHFVEARNYHHLVQVGEVKCQKTGNTLYPLRKALAVFAI
                     SEWMQELPVLVARASANTEHGEGGAIQLIVPLELLRLLHSNVKLLITQEDNEYSVEKK
                     EGLYSPWKIRSKIKEAHEIALCSEHGVVEEFIPRPET"
     gene            complement(10024..10911)
                     /locus_tag="VAH76_001235"
     CDS             complement(10024..10911)
                     /locus_tag="VAH76_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385877.1"
                     /translation="MSFFSTKNTSLQSNFIPEDGLEFSNKSQGMLYYRSDTRSPEQIF
                     KLGFSPRLPEFQNEWWKEAIKSRGYIDDLGIDYRAVDSDPSVCVCMTTKLESAPIFPL
                     SQENCYIYAIALPEFTQIKYLGKGTGDITLSRTGGTPLDPKNIVIDLHNLQSKQVGNI
                     CRFFGSQTKNLGLYAGWPLHAYEALTYRVLPQSIICAIRCQREALDLQIEVDCILGEK
                     LRLCAGKQFTFTGNIIENQHFSMGQLLYVGRPGKSKLCEMDYTLVKERAVAEITNIKN
                     QPEYCFTPNIYYGLGGKTF"
     gene            11102..12352
                     /locus_tag="VAH76_001236"
     CDS             11102..12352
                     /locus_tag="VAH76_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEO1385878.1"
                     /translation="MLFRSNKSSAHKPEQISRNYHRLMLQLKTWETNHYTPDLNEEYF
                     RTLDIETRNIQIFYNDFVNNVLIPLEQQPATDLPEFITREDIFKLLSFKIFMPLAPIF
                     AEREKWMKRIAGTDGKNAHASENYNKFKKIFLNSHHKFVIEESLSHFEHGTLKYHHEW
                     LTAKRQIAKEVKSLRALQENWKEKKNKIDRKKASILFDFLDSFESIPNQLRSREEEGF
                     KQTEVYKKALSTDKLKAIELLNQHLFDYSNPQEELAERMKLIKALYENNILFEQHHTP
                     IGWLKNQLNIETVLDKKLASLEQSLKAAENALNSYMEHAMPKTVTISTELRKESEKDS
                     IPLPEKNKSPEAVSENQNTSTSNPEIQPQTNPVQNRKNTGKVYLKVQQYEENLRRLGV
                     FTPKTPHCSESLKKDPADGNRISL"
     gene            12545..14125
                     /locus_tag="VAH76_001237"
     CDS             12545..14125
                     /locus_tag="VAH76_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEO1385879.1"
                     /translation="MFDLAKLTEFNHALVYGFEPESSPARLAGSETFEDALFPEAGQH
                     QDMLNAYLYAQETILPQIKKTGLSHITPEMLIEWVKAIHGFIGKSLMQAYDRKSGEYT
                     NEIIFRWHLGAELGTHFTLYLSGLHECKSPQQLARYLNKQFDMDYQSALDFIALLEKI
                     AKDKKYTIHESLQPYIDYSSPAIKGILVQNKLASAYNLNQLTESEKITVNKILKICMF
                     PPLIPEAMNRWARETTVQLRACDSRDLKNVSEFLATTFYQLTEVHPFANANGRTATCL
                     INTFLRAFGYPSIVLRHPGERENKDSRYQKAFAEIDSSLIPLIDLIYTRVNEAQEKAF
                     ENEKLKKLIVLRVALSDLLQETKSKHPQFNLIAFQKQVFSSPETLFAMQMADETEASI
                     FVLSMSINKLSQIAKELDLSKQKKPTLFSTAVLDSTQINAVIKSLEKISGLPGWKTNA
                     KKGLVTWLELPDMEKAKEIASELEALKVAKITVARRADNKIPVIKCEEIDCEKLVNVA
                     LQKDGKTSSKNSEFVATF"
     gene            14392..16386
                     /locus_tag="VAH76_001238"
     CDS             14392..16386
                     /locus_tag="VAH76_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1959 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385880.1"
                     /translation="MLISTRILEPALSHFNDRNNLIKQLDLSSDSDNNSFYKWVLAFW
                     LGSNITREKIIKHPLYIKCEAFIDLGYSCILVLDKQMSLLKQNSLAYKILQKNLFEII
                     QHYNDNYPLLTQNPQTLFSFFANILAKIDSKVCEDISHRKILELTQNTCLAELSRTQN
                     GPPDGLAATQVSNDVTSLMKVNPFNIGVAINKLITENFSKELFFPHYIKPTTDKHITH
                     KLLIPAWDGIVLEGLSVKGRKKTNNTVVLALIGHFQTEHHYLNTSFHEFQELFGTELV
                     LINHRNYSNRSNKFANSAEEIARDVIAFVNHFRQKNKKIVLYGMCGGAAHMILAAHML
                     SHQKIPFKLIVDRFSQKYINFVDFKTLSRARDFSRSNGQDCSRLLPGYKYYPGLMPYL
                     LILLFLLLFILVQLGLFLTKTNIDFAKLVRMIPEEDLLILQAKGEKIATSKKPFFTDI
                     IVHPENDMRAAVKDKRKQRKTILKNLCEHCLHAAGQAVFSAEMQKIFLQLFNCFDQCL
                     QLINNEKLMENTITNRPVDLHSKKLYTLTTRNKLPISQFIRGFFKQSPKMHAHLLDSI
                     KPYSSDLIVDALKQIYGNHPSMHSNLLQFSNHLALLLNDMKTNQIFISYMADRLSATQ
                     LADLNEPINVLLRSELLQLIFKSSSEQNNQDIINNHRVSI"
     gene            16571..17344
                     /locus_tag="VAH76_001239"
     CDS             16571..17344
                     /locus_tag="VAH76_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385881.1"
                     /translation="MFFSKNEKNPIKKALQGELLQDEPFIQLCTRIENFLMDTEAVNE
                     QLIELNEQLTMRLKEKGLKPGEKGATKQLRSLIQEILTEAGFREGMLQTIGNKPLKKE
                     DFMFLVSSGFMLKDSSLRGSSHGELTHAIQWCLIILKQKKDSNFLENIPVNKICDQIY
                     KKLGHKDSSNPSHPFTCWDMLIDQPGVEDARSPEWLSEHIQNDGEQIFPVLKEVIKNR
                     TKKGQTEENKEKLQKKLENPPEHYEKHEEIENILMPKPK"
     gene            complement(17376..18905)
                     /locus_tag="VAH76_001240"
     CDS             complement(17376..18905)
                     /locus_tag="VAH76_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO1385882.1"
                     /translation="MNDSENESDSEYDSDSESEYELRTLPSKIHFVWVGGPIRAQYLR
                     TIMDVAAVALKSGFEVNLWVDNEMNYIKTSTQEGIDIPNLRIRNIHELEEGMRNDPFY
                     EGDRYKKFWEYVNRERVGFRNLAAAADFFRFEILRQEGGYYFDTDTVFYLDENSQFVA
                     DEVAFGIKAHVDLYTGSLRDPHGRGPTIYHVNTLGDVNNDIIAAIPDHEVMEDAILNA
                     MQRHEEYDLSPLPRESLKKRFGPYGNLMDAKRTPYQLEGSMDFRKHQTIAAGPGALQD
                     ALRDYMEGVEVEDYNLLMTLKVGNESSRVANDKDKKPIMGIEVVSKCDKTWLTKINKA
                     KKRKARQKAFDTDSLAIEPALSKDSGQTASNREAYSDGESEYDGELAEEIEFQGNLRK
                     EQFINKLKDYIITKKEEARQALEVKENEEGHDSEEKILSKHFKTRHIELTKGFSVEQK
                     IAAVEKLISLLQEKDGAELLNAIDYAALTSSRLGKLVQRYDDLILKEYVVPDEYGEKP
                     Y"
     gene            19051..19617
                     /gene="lirB"
                     /locus_tag="VAH76_001241"
     CDS             19051..19617
                     /gene="lirB"
                     /locus_tag="VAH76_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system effector LirB"
                     /protein_id="HEO1385883.1"
                     /translation="MNKIICLLSNLLLTIGIMSAHAETAIIKTSEGNITCELFTKEAP
                     NTVANFVGLATGTKEFKDIKTGKMVKRPFYNGLTFHRVIAGFMIQGGDPLGNGTGGPG
                     YTFDNENTNASFNKPGVLAMANAGPNTNGSQFFITVAPTPELQGNYNVFGQVISGQEV
                     VDKISKMPTDPQDKPITPVVIENITIQR"
     gene            complement(19698..20045)
                     /locus_tag="VAH76_001242"
     CDS             complement(19698..20045)
                     /locus_tag="VAH76_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385884.1"
                     /translation="MNGKSGSEELNIIFNYENIKNKNPHDLFNELFKKFTEDVGYGMG
                     SRSIEALKRALAKNDITYTDGYYGKTATLTSLLWSKEPPVITDEKNIFKLLDKKHLSE
                     NKEDVLGKGIRYI"
     gene            complement(20086..>20724)
                     /locus_tag="VAH76_001243"
     CDS             complement(20086..>20724)
                     /locus_tag="VAH76_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1385885.1"
                     /translation="KESLKDQKEFCKLIDKAIKELEETKKKKIFQKEPHELVTSIINK
                     SIKLLHDLKNNMPLRREDLVIVILSTENALTAAKSKLRPQEINLSEPIKCIKNFKKIL
                     SIKQKEELCFYKSDEEKSIFSKYINSELRELVESYKVQKPCEKELVNEPEKNNSPQTI
                     ESKEEDKKIDLNKKMNLYRVQKIMKSFVFEYSGYKGGKKVKEGGGNDRRTEK"
BASE COUNT         6798 a         4072 c         3460 g         6394 t
ORIGIN      
        1 tgtgttttat ttaactactt gaagtttcaa aatgcgtctc atagacatac acaagttagt
       61 aaaggcaaat taaccggcag gtgcttatga tgccttacta taatttttga atgtaaccta
      121 aactttaagt aagcaggaca ttgggaagcg tcatgacaga cattgcggtt attatcaata
      181 atcgagctaa aaatgcacac cgaatggaag attatttatc cgaactcaga gcgcatgaca
      241 ttgattatca attatacaaa gcagaccctc aacaattgga aaagaatatt cagcattgtt
      301 gctcaaaata ccctttacta ttaatcgggg gtggagacgg tactgttcgg agtgctgctc
      361 agtggtgcag caatacgtct gttattcttg gtgtgcttcc tttaggaact atgaaccatt
      421 tttccaaaga gttaaattta ccttcaacta ctgaggaatt aatagcagca atcgtggaaa
      481 aaacaacaac cacaattgac gtagcagaag taaatggcca tatctttatt aacaactcgt
      541 ctattggctt ctattccaag ctggcaaaaa accgtgatta ttatacaaat ttttataata
      601 aatggcttac ttacattccc agttttattc aggctttgtc ataccacccc tcttatgcct
      661 tattaataaa aaataaggaa cttgatatat caatacgcac ttcctttttt atggtaagca
      721 acaattgcta tacctacgaa tttcccctca aatttacccg ggaaagtttt caaaatgagc
      781 agctgggcat ttattattac aaacgcagca aattacgttt attcaaattt ttaattgatt
      841 tcttaacaga taaggagcat tttgaaataa tacagacaag attacctctg gaggttgatg
      901 ttcttcataa aaataaaatt accatctctt tagatgggga tacgctggct atagcgcccc
      961 ctctttatta taaaatttta cctaaatcac taaaattact gactaaaaaa atatgaaaat
     1021 tatccatatt tccgatttgc attttggcaa gcatcaacct gcagttttaa atgcgtttct
     1081 tcaggaaacc gccctcgata aaccagacat cattcttatt tcaggagatt taactcaacg
     1141 tggactctcg tatcagtacc gggagctttg ctcatttata aatcaactgc cagctcaaac
     1201 gcttactgtt cccggtaatc atgacattcc cttttataat gtcccagcgc gttttatatt
     1261 cccttttcgc cattacaaac gctacataaa ctcggatgta accaccacct ttgaaaacga
     1321 ttgtgtgcgt attttgggag ttaatagtgt caaccccttg caattaaagg atggaaaact
     1381 atctcatgaa acattgaata gaattgaacg atattttaaa ccggatgatg aaaaattaaa
     1441 cttgctattc tttcatcata attttgatta tatggaagga ttacataagc cactagcaaa
     1501 tgcacaaacg cttttaagtt ttttaaagca aagtaccatt catttggttt gcacgggtca
     1561 tctacactat gctcatttaa gtttaattgc taaaaataat ggctactcct gcctggcgct
     1621 gcatgccggc tcattactgt gtccacgcag caaagataat ttaaacagct attatgtgat
     1681 tgaagcgaat cagaaaaagt gccgcattac ctggcgtgtt tttgatcaag aacactttac
     1741 tgctcgctcc atctatttta ttgattttaa taaaaaaaat gcgtcatttg agcacataaa
     1801 cccagtcaag gacgatactt gctaagacaa tgcaatgcac ttggtcaaaa taatcttcac
     1861 aggcaaaaca aataaagaat aattgttgtg tctcgcatct atagagcctc cctttaagcg
     1921 acaacaattg cctatgccaa aagaatcacc gaatgcacac gattacattg tatttctctt
     1981 ctaagaacac tagtattcca attgtcctac agcttcacac caaaagattc tgattccgat
     2041 gttgagcccc caatgaattg atttaatact ccagcaagca tgctaaaaga gccctccaat
     2101 tcattcaagc cattcaataa gtcttgaagc tgactaaaag aatcttgaaa ttcatttgct
     2161 tcttttatca tagattgagg tgtatcttgg cttaaagatg tgagcaattc gataagctca
     2221 gcatgacgcg cgttcagttt tttcaggttt ttattctttc tgcttgctcc ctgcgatatt
     2281 tgcatgattg ttgtctgaac gtctattaat tcactgcaat agctgagcat taatctttca
     2341 ttgcttaaat tttccagtat ggatttctta agctcgctaa agctaatgtc gatattaaaa
     2401 tcgattttta ataattcttt cttcaattcc tgattggctg aattaattaa ggtctgaaaa
     2461 gcaaagttct ttccggtttt atgataaaat gctttcacct tacgaactaa ctctttatat
     2521 tctttatcag ccatggcttg ttctcttgat atttcctcat cactctcaat tgcttcggcc
     2581 acagcgtcag acaatagttt tttggtaata tcacttattt catcagccaa tgcctggttt
     2641 tggggactca gaagtgagga tgagtttttt acattagtct ttaagtgctc gtatcgagca
     2701 atggactctt ccaaggcaat tttctggtca ccttcaatat catcagataa caaacatgat
     2761 tttaatgcat taattaagtg agcataaaat tgcttgttat tgatggttga acttttgttt
     2821 ttctctaacg cggcatataa ggggtgcctg gggatttcag acaaaagaag atgagctaaa
     2881 ggtaatttat gcaagccaac aggttgcatc aaacttgcgc cattttttat taatacacta
     2941 aagcagtcta ccaacgattc actatgttct gagaagcaat actcaacggc attggaatac
     3001 gttttcactt ttgttttaat tggataacta ttgataggca aacctacttt agtgactaaa
     3061 aagcttaaaa gatcagcttt tctctgagta agtgcaagaa ccattagatc attattaatc
     3121 aaaggataat aaggcgacag ggtttgagcg tattcaaact ctccagcaaa caatgctctt
     3181 tccaggagtg acttggcttc tttatttgca cggccttcga tttcatttag ggcttgtgtt
     3241 tgctctttta tcagtaactc ttccgtttcc aaattaatgt ctttgatttt ccaatgcaaa
     3301 tcaattaaaa gaactgcttt aattttttta tcttccgtct ttaatagttg agcaaacaat
     3361 ttttttatct tgctaatatc gcgctccatt ttcacatcca cagggcgttt cgctttggct
     3421 gatttggaaa ataaggtcga ttgagcaagc ccgatatttc ctctgctgat tttgggcttc
     3481 gtagcccctt gctttggaga caaggcagca acctctgctt ggtcatcttt tttctgctct
     3541 tcatcaccct tattgactgg ctcaagaatt ggttctggca tagaggatac gtacttgtgt
     3601 gttttcccca ggtaaattgc ttctctcagc caatgattac tttcagaaat ttggcttaat
     3661 gatttagtcg tcttaattaa ttcatcaagc ttttccaaat agaccgctct gtttttatcc
     3721 aaatccttac tcagtaaggt cgtttcctca tttaatctgt ggtaatcagc aagcaattta
     3781 gcttctattt ccttttgctt ttttcgcaac aattgcaaac aaggcaggcc tagctgccat
     3841 acagaatgaa tgaaatattc cagattttct ggtggagaaa ttttgacata ttgttcatca
     3901 tcatccaata actcccaggt agctgggcat gccagagcat cgaaacgatt caaaaacaaa
     3961 tgcattcgat aggtttgacc atttggatct tggaagatcg cagtaaaatg tgaaggtccc
     4021 aaatttggat tatggggatc tgtcttctca taaagactaa tatgcgcatc atgcagcctg
     4081 aaaccttctg cagcaatatt agtcaagggt aattctaaac gataatactt cttatccttt
     4141 aaggtaatat cgtgaacagg tgtaatttca tctaataatt tataatttgg agattcagct
     4201 aaaatctgcg tcaatacaaa gtaaaacaaa ttgggtttca taatctcaaa cagctcattg
     4261 aaatgagcca aggatacata aagaattaaa tttgatcaac gtgttttttt taaacccata
     4321 agctattgtc cacaacatgg aatgacatcc tcaataacaa caaacaaatg ccggtaagtg
     4381 ctgtatcgat tcaataaaaa gccaacagta tgcagttaga aagaatcagg aatgtaaaac
     4441 ctgagtttat cggatccttt cttaactcat tgaatacccc caaagtgaca gattttatag
     4501 cactttgggg aaattgaata agtagccttt caggaagaga attcatttca ttactgtaaa
     4561 ttatgcttcg gtaggaaaat cttcaaccgg gggggctatc catagactgc aatttggagc
     4621 aggcataata taagttggct tgcttaattt agctttggca gaaattttta aacactattc
     4681 tttaccagct tctgtatctg tataaaccct aagcatagcc ctgtgtggta caaactcatt
     4741 gatttaaagg attaaagcga tcacaaacat cagggtatga ataaactata aacaattaaa
     4801 aatacaacct ggacgccagt cactacaagg ccattttgta tcatggcttt gacattctca
     4861 cttccagcaa aacccattgc cgcgaacata ttggcgccag gataagcata atttgtgacg
     4921 cgagttgcaa agagcatgca caaagcaaaa gtgattagag gcaattgcat ctggaggaca
     4981 gaggggctaa agagctggtg aagcattttg atggttgctt ctgcagcgcc tggcatacca
     5041 aaagcgccgg taaagcctat taagatcgat aatactggtt gccctcccaa attaattaat
     5101 ggtgttaata gctcagttaa tgcattaaat cctccggctt gatggattag tgccataaaa
     5161 ggatcaaata aaataaacaa aaagaacaga tgcaagcttt tttgcattcc ttcaaataac
     5221 aggttaaaaa tttgtttcaa accgagtcga caggataatc cggtaatcag cgccagaaat
     5281 aacatgacaa agatcacata agaggttttc gcttgagtaa ataaaccata gacaacacaa
     5341 aacaaaaagg aaatcagaaa aattaatgta gccctttttt gttgtctatt gggagtaaaa
     5401 ggctcagtgc tgttctcgtc ttcgcaaggc tcattgatgt atagtttttg taatcgtgaa
     5461 gccataaacc aggtggtaat taaagtcact acagcaattg ggagcgaagc gtaaagaaat
     5521 actgtcccat aacttaatcc agtgacacca agcaaggtta caaccggggg agcaaaaggc
     5581 cctagaatta aagcctcttc agctgaagct tgcatgagta ccgccaaact gggacgtgac
     5641 aagcctactg accctgcaat aggacgcaaa ctaggggcca gaatggctaa acctcccgct
     5701 aatgttccca gcaaaccaac aataataaag gatgagagga ctataccaat tttagcgcgt
     5761 cgctgagtat taaccccaac tccataaaca atttggtgca ctaaagtatg acttacctgg
     5821 gtatgcgtta aaacttcgcc caaccctcga cctaacatga taataaaacc aaccaaagcc
     5881 ataaatgaac ccaaggcgtc agccatagca ttccctatgt caatgggagt gcttatattc
     5941 cacaaaaaac ctaacccaac gcaaactact gtcgctacta atggatcaac gttacgaaat
     6001 aacagtaaaa cataaagaat aataatagat aaactggcta gctgaatgta gaaagacatt
     6061 agcggatcac cccaattaga tttagtccct gtgtgatgcc ttcaatccct aatccaggtt
     6121 tgtctgccag gataatttta ttgctgagac tttgtgcccc accaagaata taattctcgc
     6181 ggatcaaaaa aataggatcg agatctgcat aagaaatatc gggcttactt aatgcaaaac
     6241 tggctattgc agcaactcca atcggcgact ccagcataca tccaaccatt atttgcattt
     6301 ttgccgcttg agcaatatgg tagatagatt gagctttctc aatgccccct gatttcatca
     6361 actttatatt gaccccatca caagcattaa tctttgcgat attcaatgca tcttctggtg
     6421 aaaaacacgc ttcgtcagcg ataattgcac aatcaacctg atcagtaatc gtctttaagt
     6481 caggcagatt tcgagcatta accggttgct ccaccacagg aacattcaaa ttctcttttt
     6541 tgaacgcagt aatggcgatt aacgcctctt cataagacca cccctgattt gcatctgcta
     6601 gcagagtaat gtcattgcct actgcctgac gaatagctcg aattcgatta atgtcgtcca
     6661 ctggatttaa tcccaatttg attttcaggg ttttgtgccc tcgctgaatt aaatcaatcg
     6721 catcacccac catctcctca acttctttaa cacttatcgt aatacatgag ttgatgcaat
     6781 tggtatttcc tcccaattgt ttataaagag gcaagccaca atattgggca aataaatcat
     6841 gcaaagcaat atcaatcgca gctttggctg aggaattacc tcctatagcc cggtcattca
     6901 tttcaagtaa taaattgaat gcagaaatat cacgtccgat aagaggaggc cccaaggcgt
     6961 ttttaatagc agcaataatg gattccttgc tatccccagt tattgcaggt gttgatgcag
     7021 ctgaaccata accaatcttt ccacaatccg tttttattaa tatcacgaca tcatcaacgc
     7081 attctgtacg cctcaccgct gtaataaacg gacggcttaa tggaatagtt agttctgcga
     7141 tatggatttc tttaatattc aaacaagcac ctgttcattt ataggttgca ggggatcata
     7201 acaatctcaa ttttttaccc aattttatta tgcattgttc tgcttttaga aaccaatata
     7261 atcaattgac aacagccata taccttgatt gataattgat agcttatcgt tattcatgcc
     7321 tgccaaaaca ccccttatct gtatttatat actgattaac ttgtaaaata tcaactaatt
     7381 ggccttatcg gagtactacc caattcattc tgctcaaaat attttaaagt acagtatttc
     7441 aaattaaaaa acatctgaga atcactctga attaaggcta tatgactaat gatcattaca
     7501 acataatcac ttgttcctgc tcaattttca ttcctcaaaa attacataag atatacagag
     7561 gccaaaaaac aaccttggaa aatcggtgtt ttttatacta gtatttaaag agaataagca
     7621 ttatttttag atatgtatga actgaatgac acgccctatt ttatatacag caatttaata
     7681 caggaatatt gttcccctct ctttaatctg acacctatta aagggtttac tttttgtcgc
     7741 acatatggag ataaaacggc ttttttatta accagtgaat atcaattcca tatagaatgc
     7801 atccaacacg aaaagaaaat ttccaaactt aatatgttgc gtacacctga gtatactcaa
     7861 gccggctatt ttcttaactc ccctgctctt gatggaaaat actctccctt ttacgaaagt
     7921 accgctcaaa aatatggtta tggatttagc tttactattg tcaggaaaaa tgagaatcac
     7981 tgcgattatt ttcattttat agggaacagg catcaaaaag atatgaataa ttattatctt
     8041 cataataaat ggttactgga taagttttgc gactattttc ttgaacgggc agcgaaagta
     8101 ttagcttaca gtgagaaaga aaatagattc acgctaacca atacagcaga acatcctttt
     8161 cctcttaaaa ttcattcaag tgcgttagat caatttttaa aggactatca ttttgaacaa
     8221 tttggactta aaccagtttg cttgcctaaa cgacaaattg aatgtgcgca gttattagtc
     8281 cgtggaaaaa cagcagagga aatagctatt attcttaact tatcaaagcg cactgttgaa
     8341 cactatatta acatcttaaa aagaaaacta aatgcccata acaaaggact ccttattaca
     8401 aaattgcttc accagtatcc tgacatgttt gaatcaattc tctaggtaga ggtaatgttc
     8461 tgtatgtaaa aatctcatgg gggaggagat gactcgagaa atttcactgg ctccaaagtc
     8521 tcagaggtga caaaaaactg ggatgtatcc ctcgcctcaa gatcttcagg ataatttttt
     8581 aactgtttta cttggcaaaa tgtgtcttct tttactctga aaagagcaaa accacattgt
     8641 ctccggcgca tgacttcgcg gattttattc attctttcat ttgaataaat gacattagga
     8701 gaataagcta aaatcaaatc atcattttgt acaggatgct tggtataagt cattacttta
     8761 taaccctttt caagaaaagt aagcccttgc ccatttctac tgaaaatctt ctccgcttct
     8821 ttctccaata ataccttagc tgactttgtt atattttttc ttaaaaaaaa actggtcata
     8881 ttttactctc ttctgacaat gacaacctac taattattac cgagaaaaaa tttaaagaaa
     8941 taccgtaaaa atacgggtta aaaaatccgg tcccataatc ttgatttcat tttaattctt
     9001 tatccttgca caagggataa agaagtttgt atacagggag taaaattagc tggagattaa
     9061 tgatatgttt tttaaaacct gtgaattcca aagaaacaaa tcaagcgata ctgtataccc
     9121 aaggattgat atacgccaag ccatctacaa ggaaagtata agtttaaccc ctgaaatggc
     9181 aatatcaatg cagaaaaaag gaggttatca aacagataat gattggaaaa aaatgctaat
     9241 gggagacgtt acccaaaatc ttctcattca atcagttatt gggaaaaaaa gcctgatttg
     9301 tgccccccct aacattaact ccatgtcctt ggaggatttc catctctgga ctaaacagcc
     9361 acttcccgct ggaaccagtc agtaccttgc gctacccaat gcattcgcgg ataatctgat
     9421 acatggatat cattttgtcg aggcaaggaa ctatcaccat ttagtccagg ttggagaagt
     9481 aaaatgccaa aaaactggaa ataccttgta cccattaaga aaagcattag ccgtatttgc
     9541 gatttctgag tggatgcagg aactacctgt tcttgttgcc agagcgtcag ccaataccga
     9601 acatggcgaa ggtggcgcga ttcaattgat tgtacccctg gaattattgc gcctccttca
     9661 ttccaatgta aaactactga ttactcaaga agacaatgaa tattcagtgg agaaaaaaga
     9721 ggggttatac tctccctgga aaatccgatc taaaataaaa gaggcgcatg aaattgcact
     9781 ctgttcagaa cacggtgtag tcgaggaatt tatcccacga ccagaaacct aatgaggagt
     9841 tatgatggcc aaggatgagc ccttttatta tctgtaaaaa cattatctta ctggcatact
     9901 ccaaaattct taattcacaa cttatccttt gggtattgat tgcaatttta attgattcta
     9961 cctaaagcca gctttattaa tgtattgcta tccacacaat tattcccagc aaaatcactt
    10021 tggttagaaa gtcttacccc caagtccata ataaatatta ggggtaaaac agtattcagg
    10081 ctgatttttt atattagtaa tttccgctac cgccctttcc ttgaccaatg tgtaatccat
    10141 ctcgcacaat ttggactttc ctggtctgcc aacatataag agctgcccca tggaaaaatg
    10201 ttgattctct ataatgtttc ctgtgaaagt aaattgtttg cctgcacata acctgagttt
    10261 ctctccgagt atacaatcga cctcaatttg taaatcgagt gcttctcgct gacatcgaat
    10321 ggcacaaatg atagattggg gtaacactct gtatgtcaaa gcctcatacg catgtaatgg
    10381 ccatccagca tataaaccaa gattttttgt ttggcttcca aaaaacctgc aaatattacc
    10441 cacttgtttg ctttgcaaat tatgcaaatc aatcacaata tttttagggt ccaaaggggt
    10501 tcccccggta cgggataatg taatatcgcc agttccttta cccaggtatt taatctgagt
    10561 gaactcaggg agcgcaatcg cgtaaatata gcaattctcc tgacttagag gaaaaatcgg
    10621 agcactctcc agcttggtag tcatgcaaac acatactgaa ggatccgaat cgactgctct
    10681 ataatctatt cctaaatcat caatataacc tctggattta atcgcttctt tccaccattc
    10741 attttgaaat tcaggcaatc tgggagaaaa acccagttta aaaatttgtt ctggagaacg
    10801 agtatcagaa cgataataaa gcatcccttg acttttattg gagaattcca ggccatcttc
    10861 aggaataaaa tttgattgta atgaagtatt ttttgttgag aaaaaactca ttctgcctcc
    10921 atttgctgac aagctcaata atgcgtgaac caattattcc caacaggcac taaaaataaa
    10981 tacagaagaa atactgtatt taaaaaaatg tatcttttgg tatcatccaa tatttataaa
    11041 atctgttcat aaagtaaaaa aattcggtca ttttattttc aaatttatcc tggagatact
    11101 catgcttttc agatccaaca aatccagcgc ccacaaacct gaacaaatta gcagaaatta
    11161 ccaccgtttg atgctgcagt tgaaaacatg ggaaacaaat cactacacac cagacttaaa
    11221 tgaagaatat tttaggactt tggatattga aacaagaaat attcaaatat tctacaatga
    11281 ttttgtgaac aatgtattaa tccctcttga acagcaacct gcaactgatt tgccggaatt
    11341 catcacccgg gaagatattt tcaaactttt aagctttaaa atatttatgc cattagctcc
    11401 gatattcgcg gagagagaaa aatggatgaa gcggattgcc ggtactgatg gaaaaaatgc
    11461 tcatgccagt gaaaactaca ataaattcaa aaaaatcttt ttaaactctc atcataagtt
    11521 cgttattgag gaaagcttat cccattttga acacggaaca ttaaaatatc accatgagtg
    11581 gctaactgcg aagcggcaaa ttgccaagga agtcaagtca ttacgcgcat tacaagaaaa
    11641 ctggaaagag aaaaaaaaca aaattgacag aaaaaaagct tctatcctgt ttgattttct
    11701 tgatagtttt gaaagtattc ccaaccaatt acgctcaagg gaagaggagg gattcaaaca
    11761 aactgaagtc tacaaaaaag cactttccac agacaagctc aaagcaatag aactcctgaa
    11821 tcagcatctt tttgattaca gcaatcctca agaagaattg gcagaaagaa tgaagcttat
    11881 caaagctctg tatgaaaata atattctatt tgaacagcac catactccaa taggatggct
    11941 gaaaaaccaa ttgaacattg aaacggtact tgataaaaag ctggcgtctc tggagcaatc
    12001 gttaaaagca gcggaaaatg cgctaaattc atatatggaa catgcaatgc ctaaaacggt
    12061 cacgatttcc actgaactaa gaaaagagtc ggaaaaggat agcatccctc tccctgagaa
    12121 aaataaatca cctgaagcag tttcagaaaa tcagaataca agtacttcta atccggaaat
    12181 tcaacctcaa accaatcctg tacaaaatag aaaaaacacc ggcaaagttt atctgaaagt
    12241 acagcagtat gaagaaaacc tccggagact tggtgtattt actcctaaaa cccctcattg
    12301 ctccgagtca ttgaaaaaag atcctgctga tggaaataga atctctctat aaaaacaagc
    12361 gaaacgcctt ttatagtctt tgtgaaagga ctcacaaata gctattgaga tagacctaaa
    12421 aaatgcaaaa gcttctatag gcgaatactt aaacctcgac aaaaggacaa ttaaagagca
    12481 cagtggatat ttattgcgtt ctctgctacg atcattcccc ttttaattaa ttacaaaaac
    12541 aaccatgttt gatttagcaa aactgactga atttaatcat gctttagtgt atggatttga
    12601 gccagaatca agccccgcac gtttggcagg ctcagaaaca tttgaagacg ctctttttcc
    12661 ggaggcaggc cagcatcaag atatgttaaa tgcctatctt tatgcacaag aaacaattct
    12721 tccgcaaatt aaaaaaacag gtttatctca tattacccca gagatgttaa ttgagtgggt
    12781 taaagccata catggcttta tcggaaaatc tctaatgcag gcttacgata gaaaatcagg
    12841 tgaatatact aatgaaatta ttttccgatg gcacctgggt gccgaactgg ggactcattt
    12901 tactttgtat ttgtcaggtc tccatgagtg caaatcccca caacaacttg caagatattt
    12961 aaataaacaa tttgatatgg attatcagtc tgctttggat tttattgctc tgttagagaa
    13021 aatcgccaaa gataaaaaat atacaattca cgaatcactg caaccctata tcgattattc
    13081 cagtcctgcg ataaagggaa ttcttgttca aaataaattg gcgagtgcat ataacctcaa
    13141 ccaattgaca gagagtgaga aaattacagt caataagatt ttaaaaattt gtatgttccc
    13201 tcctctgatt cctgaagcca tgaatcgatg ggcgcgagaa acaacggtac aattacgcgc
    13261 ttgtgattca agggatttga aaaatgtttc tgagttcctg gcaaccactt tttatcagct
    13321 tacagaagtg catccttttg ccaatgctaa tggaagaaca gcaacctgtc ttattaatac
    13381 tttccttcgc gcatttggct atcctagcat tgtgcttcgt caccctgggg aaagagaaaa
    13441 taaagacagt cgatatcaaa aagcattcgc tgaaattgat tcctctctaa ttccgttaat
    13501 tgatctgatt tatactcgtg tcaacgaagc tcaagaaaaa gcttttgaaa atgaaaaact
    13561 aaaaaaactg atcgtattgc gagtcgcttt aagtgacttg ttacaagaaa ccaagtctaa
    13621 acatcctcag tttaatctaa ttgcattcca gaaacaggtg ttttcttctc ccgagacgtt
    13681 gtttgcaatg caaatggctg atgagactga agcatccatt tttgtcctgt caatgagtat
    13741 caataaatta tctcaaatag caaaagaatt agacctgtca aaacaaaaga aaccaacatt
    13801 gttttcaaca gccgtgttgg atagcacaca aataaatgcc gtgataaaaa gcctggaaaa
    13861 aatatctggt ctgcctggct ggaaaaccaa cgcaaaaaaa ggattggtca cttggctgga
    13921 acttcccgat atggaaaaag ccaaagaaat cgcctctgaa ttggaagcgc tcaaagtagc
    13981 caaaatcacc gtggcacgac gagctgacaa taaaattcca gtgattaaat gcgaagaaat
    14041 tgactgcgaa aagcttgtta atgttgctct ccagaaagat ggtaaaacat catcaaagaa
    14101 ctcagaattt gttgcgacgt tttgataaaa aatgagcctg tatttaaaga ggcaggccaa
    14161 aaacaacaac aaattacagt gagggaaaaa atagcaggat aaataatttg tttcaattta
    14221 agcttgatct ggccaatgcc agttttttat aaaactgcgg ccggatctta ttgagccaaa
    14281 acgacaggaa atactgagca caattttttc atagcaaccc aaagatgaca tctatccatt
    14341 ttgatcgaga tagaacttaa atgaattatc ttttattacg tttttgaact tatgttaatt
    14401 tctaccagaa ttctcgagcc agcattaagt catttcaacg acaggaataa tttaatcaaa
    14461 caattagatt tatcatctga tagcgataac aattcctttt acaaatgggt tttggcgttt
    14521 tggttgggtt caaacatcac tcgcgaaaaa attatcaaac accctttgta cataaaatgt
    14581 gaagctttta ttgatttggg ttactcgtgc attttggttt tggataaaca aatgagcttg
    14641 ttaaagcaaa attcacttgc ttataagatc ctgcaaaaaa acttatttga aataattcaa
    14701 cattataatg acaactatcc gttattaacc cagaatcccc aaacgctatt ttctttcttt
    14761 gccaatattc ttgcaaaaat tgacagcaag gtatgcgaag atatttctca ccgaaaaatc
    14821 cttgaactga ctcagaatac gtgcctagct gaattatcgc gcactcaaaa tggcccgcca
    14881 gatggattag cagcaacaca agtgtctaat gatgtcacca gtttaatgaa agttaatccg
    14941 ttcaatatag gtgtcgctat caataagcta attacggaaa attttagcaa ggaacttttt
    15001 ttcccacact acataaagcc aacaactgac aagcatatta ctcataaatt attaatacca
    15061 gcctgggatg gtattgtact tgaggggctt tccgttaagg gaagaaaaaa aacaaacaat
    15121 accgttgttt tagcattaat tggacatttc caaacagaac atcattatct taataccagc
    15181 tttcatgagt tccaggagct ttttggcaca gaattagtgc ttataaatca tagaaattat
    15241 tccaatcggt ccaataaatt tgccaacagt gctgaagaaa tagccaggga tgtgatcgct
    15301 ttcgtaaacc atttcagaca aaaaaacaaa aaaattgtac tgtatggaat gtgtggtggg
    15361 gctgcgcata tgattttagc ggcccatatg ctatcccatc agaaaatccc atttaagtta
    15421 attgttgaca gattctctca aaaatacatc aatttcgtag actttaaaac actctcgaga
    15481 gccagagatt tttcacgctc aaacggacaa gattgctcga gattgcttcc aggttacaaa
    15541 tattatcccg gtttaatgcc atatttgctc attctccttt ttttattgtt atttatttta
    15601 gttcaattgg gtctattttt aacaaaaaca aatattgatt tcgcaaagct tgtgcggatg
    15661 attcctgaag aagaccttct gattttacaa gccaagggag aaaaaatagc aacctcaaaa
    15721 aaaccattct ttacagacat cattgtacac ccagaaaatg acatgcgggc agccgtaaaa
    15781 gataaaagaa aacagagaaa aaccatcttg aaaaatttat gtgagcattg tttgcatgct
    15841 gctggtcaag ctgttttttc agccgagatg caaaagattt ttcttcaatt atttaactgc
    15901 tttgatcaat gcctccagct aataaacaac gagaaattga tggaaaatac catcactaat
    15961 cgccctgtag atttacacag taaaaaactt tacaccctta caaccagaaa taagttacct
    16021 atcagtcagt tcattagagg tttttttaaa caatcaccaa aaatgcatgc ccatttgctt
    16081 gattcaatca aaccatactc tagtgacctt atagttgatg ctttaaaaca aatttatgga
    16141 aaccacccat caatgcattc taatttattg caattttcta atcatttggc tttgctttta
    16201 aatgatatga aaaccaatca gatttttatt tcttatatgg ccgatcgttt atctgctact
    16261 caattagctg atttaaatga acctataaat gtgctgttaa gaagcgaact acttcaacta
    16321 attttcaaga gttcgtctga acaaaataat caagatatca taaataatca tagggtatcc
    16381 atttaatgca ttcattgccc ccactaattg ggctatcgtt gatactaaat ggcaagatta
    16441 atgcaaatgc gcttaaacaa tgtgctcatc aacactcaac ccaaccaata agtaagcaat
    16501 aataaatatt tataaacaat atttattaaa aataatctat acttaaaaaa attagcgcgt
    16561 tggaggtaat atgtttttct ctaaaaatga aaaaaatcca ataaagaaag cacttcaagg
    16621 tgaattattg caagatgagc catttataca actatgtaca agaatagaaa acttcttgat
    16681 ggataccgaa gctgtaaatg agcaactcat tgagttaaat gaacaactga caatgaggtt
    16741 aaaggaaaaa ggactcaaac ccggagaaaa aggagcaaca aagcagttaa gatcgttaat
    16801 ccaggaaata ctgacagagg caggctttag agagggaatg ctgcaaacaa taggtaacaa
    16861 accattgaaa aaagaagatt ttatgttttt ggtatcatcc ggatttatgt taaaggacag
    16921 cagcctgaga gggtcatctc atggagaact gacccatgca attcaatggt gtttaatcat
    16981 attgaaacaa aagaaagaca gtaattttct tgaaaacata cctgtcaata agatttgtga
    17041 ccaaatttat aaaaaattag gccacaaaga ttcttcgaat ccgagccatc catttacttg
    17101 ttgggatatg cttattgatc aaccaggagt agaagacgcc agatcaccag aatggctttc
    17161 cgaacatatt caaaatgacg gggagcaaat ttttcctgta ttaaaagaag tgataaaaaa
    17221 tagaaccaag aagggtcaaa cagaagaaaa caaggaaaaa ttacaaaaaa aactggaaaa
    17281 tccgccagaa cattatgaaa aacatgagga aattgaaaat attctcatgc caaagccaaa
    17341 ataaatttga actactacaa ctaatccatt tgaattcagt acggtttttc cccatactca
    17401 tcaggcacaa catattcttt aagtatcaaa tcatcgtatc tttggactaa cttacccagg
    17461 cgagaactgg taagtgcagc gtaatcaatt gcattaagca gttccgcccc atccttttcc
    17521 tgcagtaaac taataagctt ctctacggca gcaattttct gttcaacaga gaaccctttt
    17581 gttaactcta tatgtcgtgt tttgaaatgc ttacttagaa tcttttcctc ggaatcatgg
    17641 ccttcttcat tctcttttac ttccaaagcc tgccttgctt cttctttttt tgtgataatg
    17701 taatctttca atttattaat aaattgttct ttgcgtaagt tgccttggaa ttcgatttct
    17761 tctgctaatt caccatcgta ctcactttct ccatcagagt aagcctctct attacttgct
    17821 gtctgcccag agtcctttga aagtgctggc tctatagcta gcgaatcggt atcaaaggct
    17881 ttttgcctgg cttttctttt tttagcttta tttattttag tgagccatgt tttatcacac
    17941 ttactgacca cttcgattcc catgatcggt tttttatcct tatcatttgc tactcttgag
    18001 ctttcattac caacctttaa ggtcattaat aagttgtaat cttccacttc aacaccttcc
    18061 atgtaatcac gcaatgcatc ctgtaaggca cccggaccag ctgcaatagt ttgatgtttt
    18121 ctaaaatcca ttgatccctc caattgataa ggtgtgcgtt tagcatccat cagattgcca
    18181 tatggaccga atcttttttt aagagattcc cggggcaaag gcgataagtc atactcttca
    18241 tggcgttgca tagcatttaa gattgcatct tccattacct catgatcagg aatagcagca
    18301 ataatatcgt tattgacgtc ccctaaagta ttgacatgat agatagtagg ccctcttcca
    18361 tgtggatccc tcaatgatcc tgtatacaaa tctacgtggg cttttatacc aaaagcaacc
    18421 tcatccgcta caaattgcga gttttcatcc aaataaaaaa cggtatcggt atcaaagtaa
    18481 tacccaccct cttgccttaa aatttcaaaa cgaaaaaaat ctgcagctgc agcaagattt
    18541 ctgaaaccga ctctttctct attgacatat tcccaaaatt ttttgtacct gtccccttcg
    18601 taaaaaggat catttctcat cccttcttct aattcatgaa tattccttat tcttaagtta
    18661 ggtatgtcta ttccttcttg agttgatgtt ttaatgtaat tcatttcatt atcaacccat
    18721 aaattaacct caaaaccact ttttagtgct actgcagcaa catccataat agtgcgtaaa
    18781 tattgagctc gaataggtcc cccaacccaa acaaaatgta ttttggatgg tagcgtacgg
    18841 agctcatatt cagattcaga atcgctatca tattcagaat cactttcatt ttcagaatcg
    18901 ttcatgaaac tctcgcataa taactcaata ttgtttaatt atagtacacc cctatctaat
    18961 tcagctgaaa ctctgagttt catttaaaaa ccattttaat acgacataat actgttatac
    19021 tgatcttagc cattctatgg agagcttatt atgaataaaa ttatttgttt actcagcaac
    19081 ttattgttaa caataggcat catgtcggcg catgcagaaa ctgcaattat taaaacttca
    19141 gagggcaaca taacgtgtga attatttaca aaagaagctc ccaataccgt tgcaaatttt
    19201 gtagggctgg caactggaac aaaagaattt aaagacataa aaactggcaa aatggttaaa
    19261 cgcccttttt acaatggctt aacctttcat cgtgtgattg ccggttttat gattcaagga
    19321 ggagacccgc ttggaaatgg gaccggtggg ccaggttaca catttgataa tgaaaatact
    19381 aatgccagtt ttaacaaacc tggcgtactt gcaatggcta atgcaggccc caatacgaat
    19441 ggcagccaat tttttattac cgttgcgcca acacccgaac tacaaggcaa ctacaatgtt
    19501 tttggtcaag taatttctgg acaagaagtt gttgacaaga ttagcaagat gcctactgat
    19561 ccgcaagata agccaataac gcctgtagtc attgaaaaca ttaccattca aagataaaag
    19621 atcagtgcct gtgtcagatt aggtctaaac caattcatta aatatatatt aaaactcgga
    19681 acatgggtac gtaagaacta gatataccta atcccttttc cgagtacatc ttccttattc
    19741 tcacttaaat gcttcttatc taataattta aatatatttt tttcatcagt aataacaggt
    19801 ggctctttac tccataatag acttgttaaa gttgctgttt ttccataata tccatcggta
    19861 taagtgatat catttttagc taatgccctt ttaagagcct cgattgaacg tgaacccatg
    19921 ccatatccta catcttcagt aaatttttta aagagttcat taaataaatc atgtgggttt
    19981 ttattcttga tgttttcata gttgaatatg atattgagtt cttctgaacc acttttacca
    20041 ttcatcacta tctctatttg aaaataatct ccaaaatctt tgagtttatt tttcagttct
    20101 tctatcgttg cccccccctt cttttacttt ttttcctcct ttatacccgc tatactcgaa
    20161 tacaaaactt ttcataattt tttgaacacg gtataaattc attttcttat ttaaatcaat
    20221 ttttttatcc tcttcttttg attctattgt ctgtggtgaa ttattttttt ctggctcatt
    20281 aaccaactct ttttcgcatg gcttttgaac cttgtaagat tcgacaagtt ctctaagctc
    20341 agagtttatg tatttggaga aaatagattt ttcttcatct gatttataaa agcagagctc
    20401 ttctttttgt tttattgata atatcttttt aaaattttta atgcatttta tcggttcgga
    20461 taaattgatt tcttgtggcc ttaattttga ttttgctgca gttaaagcgt tttctgtact
    20521 taagattact atgaccaaat cttctcgtct taatggcata ttatttttca agtcgtgtaa
    20581 caactttatt gatttattga taatacttgt taccagctca tgcggctcct tttgaaaaat
    20641 ttttttcttt tttgtttctt ctaattcctt aatagcttta tctatcaatt tacaaaattc
    20701 tttttggtct tttaaacttt cctt
//
LOCUS       DATNSN010000040        20544 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.040, whole genome shotgun sequence.
ACCESSION   DATNSN010000040 DATNSN010000000
VERSION     DATNSN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20544
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.040"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            165..668
                     /gene="ceg33"
                     /locus_tag="VAH76_001244"
     CDS             165..668
                     /gene="ceg33"
                     /locus_tag="VAH76_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg33"
                     /protein_id="HEO1385886.1"
                     /translation="MAAQLDPSSEFAALVKQLQREPDNPGLKQAVVKRLPEMQLLAKT
                     NSLALFRLAQVYSPSSSQHKQMILQSAAQGCTNAMLSACEILLKSGAANDLITAAHYM
                     RLIQSSKDSYIIGLGKKLLEKYPEFAEELKSKSKEVPYQSTLRFFGVQSESNKENEEK
                     IINRPTV"
     gene            785..1576
                     /locus_tag="VAH76_001245"
     CDS             785..1576
                     /locus_tag="VAH76_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO1385887.1"
                     /translation="MQTRFTYLPVTSLQAGQYQPRQDFNVTELQELAQSISSQGLIEP
                     LVVRPIAKERYEIIAGERRWRAAKLAGLNEVPCLIGDYSDQQACALTLIENIQRQDLN
                     LIEEATGYKRLIEEFHYHQDEIAALVGKSRSHVANILRLLTLTDKVKQLVRDKVLSFG
                     HARVLVGLNPEQQEMFAHQIIGEEWSVRHLEQEIKFYKNKDMNSPKNPRKDRDIERLQ
                     TILAEQVGAPVQIINDGEDGGWLKVKFFDNDTLAGLLERLGLRYD"
     gene            1668..3461
                     /gene="dacB"
                     /locus_tag="VAH76_001246"
     CDS             1668..3461
                     /gene="dacB"
                     /locus_tag="VAH76_001246"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEO1385888.1"
                     /translation="MKRTLTGAFMWAVWSLSSHAASMQFEVDKLINRLNPHVNLGIVV
                     TDLTSGETLYKRNANRLYIPASNMKLFSEAAALMALGPDYQFKNQLSTSANQLQQGVL
                     HGNLYLHLSGDPSFSREDLKTLLSSLKDWNITTIQGNVIIDSSLMSIPAYPPGWLTSD
                     LSYSYGAPIAPLMVDSNRLTITVNPGAKAGAPAIVEVDDGGGTINLNNQATTKASEKG
                     CGVGFYLDPENNLTVRGCVGLGQWAVQQRIAIKNPFVYAQGMIVSELAKSNIKLNGQV
                     LLGRAPAGTLLIATRYSKPISQLMADTLKPSDNLYADSLYLHAAAKIKGSPVDWKQAQ
                     PVIKNFLQQQTGIDLKDSNFTDGSGLSRYNLVTPAQTMALLKFLYQRFPLSYEYIAAL
                     PISGRDGTLQKRFKTPNQQGFVRAKTGTMTGMNSLSGYLYTANGHTLAFAMYINRLPG
                     KPAGPGRPLLDALCTYFLQQSPTSSRLARVLSPHSRIKFQFNPTQIELQRVHQAKWRR
                     LETAVRQVLRGQDVNVVYRGNELIVTDNQSNANSVWKALQSIGKKYSFAVALSSKVMP
                     VTPSGKPLLLWVQAPLSENKAERTWIIREAV"
     gene            4273..5430
                     /gene="legS1"
                     /locus_tag="VAH76_001247"
     CDS             4273..5430
                     /gene="legS1"
                     /locus_tag="VAH76_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegS1"
                     /protein_id="HEO1385889.1"
                     /translation="MNFSIRVILLLTCFVSLAFSAPKFLTISDIHYGAENIAKDGQDT
                     GKEFLDVTFKRFEELSQNVDFILHLGDLPTHSLFSTSKKEEYEQVVFHGLHEANPGQK
                     PMFYITGNNDSLLGNYQPFSSDNKSPLNLALDWDGACVHCDGLIIDDTHMRKDGYYSS
                     YVIPNNKEIILIALNSVQWTKTPVFLPKYPNQQRDAFVQLFWLEQQLKNHRAKQLLLA
                     MHVPPGTAYNGNRFWHEIYLDRFLTLLDKYHRSYDQITILFSHSHMEEFRKIKLSDGS
                     TIYAFSTPGISRAHHNNPGMKIFDLDKQMRVRNFTTYFTSDLYEWGNEHYQAMNIPEA
                     IFPNCQNKTIPQCLDTLSPEQVCNNLEAGLFYGVKSTRVPYQGCIKTYQIN"
     gene            complement(5447..6997)
                     /locus_tag="VAH76_001248"
     CDS             complement(5447..6997)
                     /locus_tag="VAH76_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO1385890.1"
                     /translation="MAQKPTKITHLVVLGDSLSDRGTLNKRELLGFIPMSYLSGLRSK
                     SPKGRFTNGFLWGDYVGATTAEQFEIEHIRRKLRLHNNARDNADIGDELLINDKARRE
                     NENSFSLNEDNHILFKGERFARFYCEGGLTSHDYSCSFTFNLVLFFTRLILATLGGKR
                     TQLLTDDKKYNISNLEKSETLVVEWSGANDLITANDKPSRAAAEKAVNDRIQNIEALI
                     HNGYRNFVLFNLPNLSLTPRFQRKNADEQRNASDCTEYFNQQLQTKSLELIKKYKELG
                     IPVNLSVFDVNEQFKEVYSNPEQYGFDRDKLKSPYVESDLFKENQKNPIDQKEHISPA
                     KGYMFWDDVHPTATMHDWLAERFKEKYYDKVFRFEPPLQAKHRHKETADALAKTTDSM
                     IKTDKPIKKLPEDIVLMLNAIHSRAQLMCQSTDAQRREKGELLKHFIFEIKCQHGNLE
                     NIYGLISAFTLNPDHSKIIKTHQNPMIDFFRNKVTTRSEDDIAALQKAVATYLQPKES
                     NEHLVKLT"
     gene            complement(7218..8312)
                     /locus_tag="VAH76_001249"
     CDS             complement(7218..8312)
                     /locus_tag="VAH76_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEO1385891.1"
                     /translation="MRVSKLLLLTLALSGNLFAQDIKVVGTLNQTLKAPQNKTLNTKP
                     AIQQIKLLKMQLSNSAIKALARKTDITLKKPNTIATSTALPSKVELGMNNVPVLNQGS
                     FGTCVTFAATAAVDAALNKGDYISQLCQLQLGLYLEKNGHSPSGWDGSLGRIVLSQME
                     NFGIVSKEKQKNLGCGGLTEYPDNEQPTPDHAMSLEQFHEMSEDPETNPVIYTPILDV
                     YQAVLDRTDTNKTINDIKTALNQKDRVTFAVLLLDFDLGVMGAVGTNKAQFDTWVLTP
                     EIARDIYLRPFFGGHEMIITGYDDNAVAVDDNGREHKGLFTLRNSWGEQFGDKGNFYM
                     SYDYFKVLTIEAQRIRALADNESEEESMTA"
     gene            complement(8515..9246)
                     /locus_tag="VAH76_001250"
     CDS             complement(8515..9246)
                     /locus_tag="VAH76_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEO1385892.1"
                     /translation="MKKISALIFLYLISTASFSLPKGFVYLHDVAPDIIEDIRYATHN
                     NFIGNPIPGYKRGVCIVTRQTAQQLKKAEEYIKSKGYTLKVYDCYRPQRAVDYFYKWS
                     RNAKDNRMKPEFYPREIKQDLFDRGYIALTSGHTRGSTLDLTLVKLTASRENKSNHPL
                     TRCYDKKPNYLDDDSIDTGTRFDCLDVSANIDYSKLSKNQKANRKLLQKLMSRFGFRP
                     YYYEWWHFTLKNEPYPDTYFNFPVE"
     gene            complement(9272..12010)
                     /gene="sidF"
                     /locus_tag="VAH76_001251"
     CDS             complement(9272..12010)
                     /gene="sidF"
                     /locus_tag="VAH76_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidF"
                     /protein_id="HEO1385893.1"
                     /translation="MPRITENIETYVTHLLSDLEPVPSQSQHLAYGYPGERQVFKDPM
                     LDGKQVVVVNSQYDKHGKPVTGQPDVIQEANNYIDNLVAAAKSLIDKDNKDKKDSRKN
                     TIDEMAKTFKKSISETIPSDKAKADLFSSKKSSANKDFLEQLAKVEGSLQQFTQAVAK
                     ASGHKLKALDKEGHNIRSHNRDTLIHRFKAPNSKEGEEQFIMYIPCGRYTKRQQRLMG
                     KDESEVNRDHDTTSHKRSDLVLGNSSMARMIAGTRHSDGTVTIHHDSFSGPGARMPYS
                     DFKGADDYKKLAIKAVTLINQEEVIQTLAQRQIDRMTNEDLWNKIPEEYRPDELPPDA
                     EKARAQLIKLYVEHNPLSVTECYTQVVTAGQRVAAENQKEQFEYVRQMMDAFDGSKAK
                     ITIQTGSNTEVETEVGYQARMSSWGVNWFRQVGALNPLSDNSVTKNQNARFVNQMTDD
                     VISNLDKVAQNLSDYDKAGALHTLLKGPDVSDLNQQITEKENALKEVKGAYRETLFSY
                     FEEYQKGEGKWDQAKLDQLKNQVDGYEKSIKKQESAIYELHNQIDALRKAYHTEHKGQ
                     INKALQELKEQISPVIQNKETDPETKSRLQHFYNSCAYLMQAQELYYENTWHHGKNNF
                     KLQTLMASLAGELDYANTKGCKSNNDRGQRLAQKIVGNALWTAMSEDGLFTGEFLDHR
                     RTHGKEVSKVEQLDRELTTIQALHHTANTGVSGGKFEIQDKANFADNGLFGKIANFAK
                     VKEMGPENAFTKNMGTKIKAGLGLGLLAAGGLVALTLLFPPAGLAVAALGIGVAAGAA
                     IVAGVTFAATYLFGTAIDAINNKIEEGKIKSIVREGMKAASENSPQHKTEQKDHLTVN
                     AKLHQQLGVTRESVLERQSTPKQEFEKPKDMTSKVDLATEVEDDKRSESTPTPVNF"
     gene            complement(12174..12353)
                     /locus_tag="VAH76_001252"
     CDS             complement(12174..12353)
                     /locus_tag="VAH76_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385894.1"
                     /translation="MKWIMLIVVIITTAGCCCTTAEVVEYQQVTVAAPVVEPVILDVD
                     DPEPLDVTTTTIEFY"
     gene            complement(12476..12742)
                     /locus_tag="VAH76_001253"
     CDS             complement(12476..12742)
                     /locus_tag="VAH76_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEO1385895.1"
                     /translation="MKRKKPVAVALRYDGNSPPKVTAKGEGLFAQQIINTAKEHGIPL
                     EQNPELTALLSQVRLNEEIPKALYIAVAQILAFLYYINGKHSKE"
     gene            complement(12739..13905)
                     /locus_tag="VAH76_001254"
     CDS             complement(12739..13905)
                     /locus_tag="VAH76_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEO1385896.1"
                     /translation="MSVEMSKTSSVRFPPGQEIKLAKPATELYVGQILKTVVVTTLSN
                     DQVLININGQNLNAKASHHFTPGELFEVKVIANHPETILEVQQKTPLPSLLQNALLQY
                     LPKQAPATNLLQTLTQLMAENNLSAPINQVMKAILGNVPVLTQLPNQLMQAISQSGVF
                     LESKLLKGKIPREIQSDFKGLCFKLLHSLGTDSKLPINLPNELESGFLQQAPLPLPGA
                     IPQPLTKDTILNLIGLSAEAVHHILREQVTQALARITTNQINYLSQENQEAYLIMLDF
                     PLKTPDGIDVIPIMIKQHKAEPMQLSKWSISFALNLSHLGSIQATVSIYAKNIDIKVN
                     TQQSSTLQLLNDYHKDLEETLYESGLHLREWKLQVGLEENHIDATNLRLLDIKI"
     gene            14143..16380
                     /locus_tag="VAH76_001255"
     CDS             14143..16380
                     /locus_tag="VAH76_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385897.1"
                     /translation="MLDRASVVDEQFLKRVLQGDFPQLRSSMSPDTAELDKKTAIEFF
                     DSQIKSRLLDIIARQLKEKGLSFYTIGSSGHEGNAVLGQVFRASDMAFLHYRSGAFFL
                     QRAKQLPGIDGVKDILLSLVAAANDPISGGRHKVFGSVPLNIPPQTSTIASHLPKALG
                     AALSITRARELGLPSKLAPDSVILCSFGDASTNHATSQATLNACSWITQQNYPLPIVF
                     ICEDNGIGISVPTPSSWIKSSVSSRPGIHYIECDGLNIADVFAKAQEADYLARVKKQP
                     VFLHMRCVRLLGHAGSDIESQYNTQEEIEQREANDPLLHTAGILYKEGWMSLQAMVEL
                     YQDNRALIEAKALEAIREPRMSSAEEIMASIVPRVEKKPKYPLPDDNRREQVFAGAYN
                     QLTLKRNLCQQINFALTDLMMQYPNMLIFGEDVGKKGGVYRVTADLQARFGQRRVFDT
                     LLDETTIIGTAIGLAHNGFIPVPEIQFLAYLHNAEDQLRGEASTLSFFSSGQYQNPMV
                     LRIASLAYQKGFGGHFHNDNSIAVLRDLPGVIVACPSNGPDAARMLRTCMRLAYEEGR
                     VVVFLEPIALYMTKDLYSPGDNGWLFEYPSPDEMISQGEVGVYGEGDTVILTYANGYY
                     LSRQAEKVLREAHNISVKIVDLRWLSPLPKDAILKEIAKAKRILIVDEGRQSGSISEG
                     LMTLLMEEASPRLKIKRITGKDCFIPLGTAWQYLLPSQESIIDAVIALQSDKREKESG
                     RLVVS"
     gene            16358..17515
                     /locus_tag="VAH76_001256"
     CDS             16358..17515
                     /locus_tag="VAH76_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEO1385898.1"
                     /translation="MDDLLFLDEQLHDDERMIRDSVSRFVSNDVIPLMAESYEHAHFP
                     KELIKKSADLGLLGLTLPAEYGGAEASYVAYGLVCQELERGDSGLRSFVSVQSSLCMY
                     PIFKFGSEEQKKRYLPEMAAGNIIGCFGLTEPDSGSDPASMRTHAKKIEGGWVLNGAK
                     MWITNAPIADIAIVWAKTEAGIRGFIVDTKSEGMSCPEIKLKMSLRASITGELVFDNV
                     FVPDENLLPKSEKGLGAALSCLSQARYGIAWGAMGAAMACFDIARNYLLERKQFAKPL
                     ASFQLIQKDLADMYTEIIKAQCLNLQIGRLKDQHRETPTMISLAKGNACREALKIARK
                     CRNLMGGNGISLEYHVIRHMLNLESVFTYEGTDNVHTLVLGRHITGINAFG"
     gene            17711..18124
                     /locus_tag="VAH76_001257"
     CDS             17711..18124
                     /locus_tag="VAH76_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385899.1"
                     /translation="MDIRCKTVLYKQLQEFNMINMKLKATLIACLSVLTLSSYANSSE
                     NKEAILQQCHDLASTVASLVSSQAKKTCAEKLVIASLHIDTAADWIVEDVHSAAKQEL
                     DNAIYSLQYAELNSCNRYIQISHSKLEAQRIKSLL"
     gene            18367..18621
                     /locus_tag="VAH76_001258"
     CDS             18367..18621
                     /locus_tag="VAH76_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385900.1"
                     /translation="MIELEPEPDTVLDPKDILTEEMKLEGSDNDMDHNQFTRGDEVDL
                     VGDGVPGYETTGQDGTDDEIEEQREARYTPTLNPNPKNVP"
     gene            18717..19469
                     /locus_tag="VAH76_001259"
     CDS             18717..19469
                     /locus_tag="VAH76_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385901.1"
                     /translation="MLVTPRFKIMVVAKKREETQKDERARAIENNPCSICRSLGLPFC
                     RGHGGGSGGGGSDSASDDKADDNDHQLLASKNPANVTNIEQLLLHSGLWHLPEDEDFV
                     FEFKDPLALVNMTLNMELGLLVLQGKRELGNEEQNALDELYETIAQEFNLFKNSIKDP
                     QVPLETMTLAREGNSLTIKIPSPKYYDLFVQQLMEKNLLPGNAPVLQHQNQSVKHPEK
                     SEAAKDSVMEEYKSSLPNPFDISRGPKLPDWI"
     gene            19772..20149
                     /locus_tag="VAH76_001260"
     CDS             19772..20149
                     /locus_tag="VAH76_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEO1385902.1"
                     /translation="MTTPEITLSRVYSPPEHKEGLWILVDRLWPRGIKKESIPLDMWL
                     KDIAPSPALRKWFHQDSEHWHEFAKQYAQELKEKPELIEHILALASTSPVTLFYAAKD
                     LQHNHALVLQKVLKSWPKIPEIK"
     gene            20393..20469
                     /locus_tag="VAH76_001261"
     tRNA            20393..20469
                     /locus_tag="VAH76_001261"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20427..20429,aa:Pro,seq:ggg)
BASE COUNT         5843 a         3867 c         4368 g         6466 t
ORIGIN      
        1 cgcgttttga tcaagatggg ggttttttta gcatttatgc ccttaaaata ctgttaaaaa
       61 aacagttatg tgtaaaaaaa atgtaaaagt gccatctctt aattttgtct taattttgag
      121 catttattct gtctttaatg aagatgataa tcaaaggtta aagtatggcc gcgcaattag
      181 atccaagtag cgaatttgct gctttggtta aacaattaca acgagaaccc gataatccgg
      241 gattaaagca agccgtagtg aagcgtctgc ctgaaatgca gttattagct aaaacgaatt
      301 ctttggcttt atttcgtctg gcacaagttt attctccttc atcatcacag cataagcaga
      361 tgattttgca atcagctgcc caaggttgca caaatgcaat gttatctgct tgtgagatac
      421 tattgaaatc cggagctgca aatgacttga ttactgctgc acattatatg cgcctcattc
      481 aaagctccaa ggattcgtat attattgggc ttggcaagaa actattagaa aaatatcccg
      541 aattcgctga agaattaaaa tcgaaatcca aagaagtacc ttatcagtcc actcttcgat
      601 tttttggagt tcaatctgaa agtaacaagg aaaatgagga aaaaataata aacagaccca
      661 ctgtttagaa taagtttttt ctcgtttcag ggtttttctt tgtggccaga taaaccggat
      721 acaattccat ttcaggcact attgtgaaac aatttcatat tcttaagttt cttgattttt
      781 tcctatgcaa acccgattta cttatttacc tgtcacaagc ctgcaggcag gtcagtatca
      841 acccaggcaa gattttaatg taacggagtt acaggaattg gctcagtcaa tttcttccca
      901 gggtttaatt gaacctttgg tggtaagacc catagcaaaa gagcgatatg aaattattgc
      961 aggggaaaga aggtggcgtg cggccaaatt ggcggggtta aatgaagtcc cttgtttgat
     1021 tggagattat agtgatcaac aggcatgtgc tctcacttta atcgaaaata ttcaacgtca
     1081 ggatttgaat ttaatcgagg aagccactgg ttataagcgt ttaattgaag aattccatta
     1141 tcatcaggac gagatcgctg cgttggtagg taaatctcgt agccatgtcg caaatattct
     1201 tcgtctcctt accttgacag ataaggttaa acagttggtt cgagacaaag tgttatcctt
     1261 tggccatgct cgtgtattgg ttggcttaaa tcctgaacaa caagaaatgt ttgctcacca
     1321 gattattgga gaggaatggt ctgtcagaca cttagagcaa gaaattaaat tttataaaaa
     1381 taaagatatg aatagcccaa aaaatcccag aaaagacaga gatattgagc ggttacagac
     1441 tatcttggct gaacaagtgg gagctcctgt acaaattata aacgatggcg aagacggagg
     1501 ttggttaaaa gtaaaatttt ttgataatga tacccttgca gggcttttgg agcgtttggg
     1561 cttgagatat gattagctta tcggaactaa ctcagaaatt gtcactatgt atcaaacaat
     1621 tcaaactggt ttatattgtg aaattcaatg ttaaggaaaa tatttaaatg aaaaggacgt
     1681 tgactggggc attcatgtgg gcagtatggt cactgtcatc tcatgcggca agtatgcagt
     1741 tcgaggtgga taaattaatt aatcggctaa accctcatgt gaacctgggg atagtagtga
     1801 ccgatctaac ctcgggcgag acgctatata aacgcaatgc caatcgtttg tatattcctg
     1861 ccagtaacat gaagcttttt tccgaggcag cggccctgat ggctttgggt ccagactacc
     1921 aattcaagaa tcaactcagt acgagcgcca atcaattaca acaaggtgtt ttgcacggaa
     1981 acttgtattt acatctcagc ggcgatcctt cgtttagcag agaagattta aagacgttgc
     2041 tttcttcact caaggattgg aatatcacaa ccattcaagg taatgtgatc attgacagta
     2101 gcctgatgtc cattcccgcc tatcccccag gctggctaac ttctgattta tcttatagtt
     2161 atggtgcgcc aatagcacct ttgatggttg attccaaccg attaacaatc acggtcaacc
     2221 ccggggctaa agcaggcgct cctgctattg ttgaagtaga tgatggcggc ggtactatta
     2281 acctgaataa tcaagcgacc acaaaggcaa gtgagaaagg gtgtggtgta gggttttatt
     2341 tggatcctga gaacaattta acggtaagag gctgtgttgg attagggcaa tgggctgttc
     2401 agcaacgcat cgctataaag aatccttttg tttatgccca ggggatgatt gtgagcgagc
     2461 ttgccaagtc taatattaaa ttaaatggac aggttttgct tggaagagcg cctgctggta
     2521 ctttattaat tgccactcgc tattcaaagc caatttccca actgatggca gatactttaa
     2581 aaccttctga taatttatat gctgatagtt tgtacttgca tgctgctgcc aaaattaagg
     2641 gtagtcctgt agattggaag caagcacagc cagtaattaa aaattttttg cagcaacaaa
     2701 caggaatcga tttaaaagac tcgaatttta cagatggttc tggattatca cgttataatt
     2761 tggtaactcc agcgcaaacc atggcgcttt taaaattctt gtatcaacgt ttcccattat
     2821 cttatgaata cattgctgct ttacccatct ctggtcggga tggtactttg caaaagcggt
     2881 ttaaaacccc aaatcaacaa ggatttgtgc gggccaaaac cgggacgatg acgggtatga
     2941 acagtttatc cggttacttg tatactgcta atggccatac cttggctttt gctatgtata
     3001 tcaatagatt acccggaaaa ccggctgggc caggacgccc attattagac gcattatgca
     3061 cttatttctt gcagcaaagc ccaacaagca gtcgactggc acgggtatta tctccccaca
     3121 gtcgcattaa gttccaattt aatcctacac aaattgagtt acagagagtt catcaagcca
     3181 aatggaggcg tttggaaacg gcagtgaggc aggtcttgag aggacaggat gtgaatgtgg
     3241 tttaccgggg caatgaatta atcgtgacag acaatcagtc taatgcgaat agcgtttgga
     3301 aagctttaca atcaattggc aagaaatatt cattcgccgt agccttgtct tctaaagtga
     3361 tgcctgttac accatcggga aaacctttgc ttctttgggt gcaagcacct ttatctgaaa
     3421 ataaggcaga gagaacctgg ataattcggg aagcggtata acccgagttt gataattaaa
     3481 aaaaagacca gcctcgtatt actaaaatgc aatgcggggc tagtacaatc attccataag
     3541 ttatggataa gatcagtcgt tatctcgaac cgtttgggct caggaggcat ttatgtcgtt
     3601 tcgaagcctt gtatgagatt taatattttg tgccaaggct tcgagacgat gcttatgcat
     3661 cttctcagcc tgaacggccc ctggatatcg actggttctc atataatcct tatccattta
     3721 ttgccgcgct gcaaacatgt ctgcggtcct gatcaactga ttttgaattg atttgagctg
     3781 aggcatcatc agggattggg ttttgaatgt gctgttctgc ccaaaacatg atttaaataa
     3841 cgtgaagtta tggataagat cagttgttat ctcgaaccgt ttgggctgag gaggcattta
     3901 tgccgtctcg aagccttgta tgggatttca tatttcgtgt cagggcttcg agacgatgct
     3961 tatgcatctc ctcagcctga acggaccctg gatatcggca ggttctcgta taatccttat
     4021 cggtataact catgttaaat agattgttat ttctgcattc gggctttgca gtattcaagg
     4081 tgattgtaac agtacttacg aaaacgccaa ggtcaaggca aaaaatgttt ttaatgcggg
     4141 agtttagata aactaaatgg ccgaattaaa aacattttta aacaaagaga ttgaggttta
     4201 tcgtaagtcc tgtataaaag agctataatt gagaactgaa taatttcggt ataaattggt
     4261 ttggagtagt aattgaattt tagtattcgg gtaattcttt tattaacatg ttttgtttct
     4321 ttagcctttt ctgcaccaaa atttctaaca atctctgata ttcattatgg tgcagaaaat
     4381 atagcaaagg atgggcagga tactggaaag gagtttttgg atgtcacgtt caaacgattt
     4441 gaggaattgt ctcaaaatgt agattttatt ttacatttgg gtgatctgcc tactcactcg
     4501 ttattttcga catctaaaaa agaagaatac gaacaagttg tatttcatgg tttgcatgaa
     4561 gcgaatccgg gtcaaaagcc catgttttat attacaggaa ataatgattc cttgcttggc
     4621 aattaccagc cattttcttc agacaataag tcgccactca atttggctct ggattgggat
     4681 ggtgcttgcg ttcattgtga tgggttaatt attgatgata ctcatatgcg caaggatgga
     4741 tattactcca gttatgtcat ccccaataat aaagagatta ttttaatagc acttaattcg
     4801 gttcaatgga ccaagacccc tgttttttta ccaaagtacc ctaatcaaca aagggatgcc
     4861 tttgtacaat tattttggct tgaacaacaa ttaaaaaatc atcgtgcaaa gcaattgttg
     4921 ttggcgatgc atgttcctcc tggtacagcc tataacggca accggttttg gcatgaaatt
     4981 tatctggata gatttttgac cttattagac aaatatcatc gatcctatga tcaaatcacc
     5041 atactgttct cacactccca tatggaggaa ttcaggaaaa tcaaattatc cgatggctca
     5101 actatttatg cgttttctac tcccggaatt agcagggcgc atcacaacaa tcccgggatg
     5161 aaaatttttg atttagacaa gcaaatgagg gtcagaaatt ttactactta ttttacgtcc
     5221 gatttgtatg agtggggtaa tgagcattat caagccatga acattcctga agccattttt
     5281 ccaaattgtc aaaataaaac gataccccag tgcctggaca ccttaagtcc agagcaagta
     5341 tgcaataatc tggaagcagg actattttat ggggttaaaa gtacgcgtgt accctatcaa
     5401 ggatgcatta aaacttacca gatcaattaa gttaggattt cgtttatcag gtaagtttaa
     5461 cgagatgttc attgctttct ttaggttgta aatacgttgc gaccgctttt tgcaaggcag
     5521 caatatcatc ttcgcttctt gtcgttacct tatttctaaa gaaatcaatc attggattct
     5581 ggtgggtttt tattattttt gaatgatcag ggtttaaggt aaatgcggag attaacccat
     5641 agatgttttc aaggtttcca tgttggcatt ttatttcaaa aataaaatgc tttaataatt
     5701 cacctttttc acgtcgttga gcatcagtgg attgacacat caattgtgct cttgaatgga
     5761 tagcattcaa catcaataca atatcttccg gaagcttctt tatcggctta tcggttttga
     5821 tcatggagtc cgttgttttt gccagagcat cagcagtttc tttgtgccta tgctttgcct
     5881 gaagcggtgg ttcaaatcga aaaactttat catagtattt ctctttgaaa cgctcagcta
     5941 accagtcatg cattgttgca gtcgggtgaa catcatccca aaacatatat cctttggctg
     6001 gtgaaatatg ttctttttga tcgataggat ttttctgatt ttctttaaaa agatctgatt
     6061 caacgtaagg ggattttaac ttgtctctat caaagccata ttgttcggga ttactgtaaa
     6121 cttccttaaa ctgctcatta acatcaaaga cggagagatt gacaggaatt cccaattctt
     6181 tgtatttttt gattaattca agagattttg tctgcagttg ctgattaaag tattcagtac
     6241 aatcggatgc attacgctgt tcatcagcat tttttctttg aaatcttgga gtcaatgaga
     6301 gattgggcaa attgaataaa acaaaatttc tgtacccatt atgaataagc gcttcaatat
     6361 tttgaattcg gtcgttaacg gctttctctg cagccgcacg agaaggcttg tcattggcgg
     6421 tgatcaagtc gttcgcgcct gaccactcca caactaatgt ttcagatttt tcaaggttgg
     6481 aaatattgta ttttttatca tcagtcaata attgagtccg ttttcctccc aaagtagcga
     6541 gaatcaacct tgtaaaaaac agaaccaggt taaaggtaaa gctacaagag tagtcatgag
     6601 aagttaatcc accttcgcaa tagaagcggg caaatctttc ccctttaaac agaatatgat
     6661 tgtcttcatt taagctaaaa gagttttcat tttctcttcg cgccttatca ttgatgagta
     6721 attcatcgcc tatatccgca ttgtctcttg cattattatg caatcttaat tttcttcgga
     6781 tatgctctat ctcaaattgc tctgcagtgg tcgcaccgac ataatccccc caaagaaaac
     6841 cattagtgaa tcgacctttt ggagatttgc ttcttaatcc acttaaataa ctcatgggga
     6901 taaaacccag cagctcacgt ttattaagcg ttcctctatc ggataaacta tctcccaata
     6961 ctacaaggtg tgtgatttta gttggttttt gggccatgat atttgcaaga aaaataacgt
     7021 gatcacatta tacacaaaat gatttttaaa tggtatgttt tggactgctc ttctggtgaa
     7081 atttttattg cggaggacgc aaaatagctg aatcaagttt gatttaggaa taccctcacc
     7141 taatccactt gcaaagtgaa ccaggatgag gggagtttag cttaaccgat tatattgatt
     7201 ttaagatggt gatttgctca ggcagtcatt gactcttctt ctgattcatt atcagcaaga
     7261 gcacggatac gttgtgcctc aatagttaaa accttgaagt aatcatagga catgtaaaaa
     7321 ttacccttgt ctccaaattg ctcaccccat gagttacgta aagtaaataa tcctttgtgc
     7381 tcccggccat tgtcatcaac agcgacggca ttgtcatcat atccagtgat aatcatttca
     7441 tggccgccaa aaaatggacg aagataaata tcacgagcaa tttcaggggt cagtacccag
     7501 gtatcgaatt gtgctttatt cgttcctacg gcacccataa cacccaaatc aaaatccagc
     7561 aataatactg caaaagtaac tcgatctttt tgattcaaag cggtttttat atcattaatc
     7621 gttttgttgg tgtctgttct gtctaataca gcctgataaa catccaggat gggggtgtaa
     7681 ataacaggat tagtctctgg atcctcgctc atttcatgga attgctcaag cgacatggca
     7741 tgatcagggg taggttgctc gttgtctggg tattcagtta gtccaccaca tcccaggttt
     7801 ttttgttttt ctttgctgac tatcccaaaa ttttccattt gacttaagac tatgcgccct
     7861 aatgagccat cccaaccact tggtgaatga ccgtttttct ccagatacag tcctagttgt
     7921 aattggcaca attgactgat gtagtcacct ttgtttaaag cagcatctac ggccgcagtc
     7981 gctgcaaaag tgacgcaagt tccaaagctt ccctggttca gaacaggtac gttattcata
     8041 cctaattcta ctttggatgg gagagctgtc gatgtggcta tggtattcgg ttttttgagt
     8101 gtaatgtctg tttttctcgc aagtgccttg atagcagaat ttgacaactg cattttaagc
     8161 aatttgattt gttgtatggc gggttttgta ttgagtgttt tattttgagg ggcttttaat
     8221 gtctggttta acgttcctac tactttgata tcttgagcaa ataaattccc gctcaatgcc
     8281 aaagttaata aaagtaattt tgaaacacgc atctatcatt ctccgttagt gacatggact
     8341 tgaaatggtt ggcaagaata actgatgcgc aaataatatt caatatatat ttaatatgcc
     8401 catgaaagaa aaaataagcc catattgggt tttaattgtc ttgcaagggt tggattgatc
     8461 tgtttcatcc aggacatgaa taagctggtc agagttaatt ctgatgttaa ctatttattc
     8521 gactggaaaa ttgaaatagg tgtctggata aggttcgttt ttcagggtga aatgccacca
     8581 ttcatagtaa taaggcctaa aaccaaagcg gctcatgagt ttttgtaata atttgcgatt
     8641 ggctttctgg tttttactca gcttggaata atcaatgttg gctgacacat ccagacaatc
     8701 aaagcgagtt cctgtatcga tcgaatcgtc atcaagatag tttggctttt tatcgtagca
     8761 acgtgttaaa ggatgattag atttgttttc tcgagaggcc gttagtttca ccagagttaa
     8821 gtcaagggta ctgcctcgcg tgtggccaga ggttagagca atataaccac ggtcaaacaa
     8881 atcttgtttt atttcacggg gataaaattc aggtttcatg cgattatctt tggcattgcg
     8941 gctccactta taaaaataat ccacagccct ttgtggcctg tagcaatcat atactttaag
     9001 cgtatatcct ttggatttga tgtattcttc cgcttttttt aattgctgcg cggtttgtct
     9061 ggtgactata catactccgc gtttgtaacc tgggatagga tttcctatga aattattgtg
     9121 ggtggcatat ctgatgtctt caatgatatc aggcgctaca tcgtgtaaat aaacaaagcc
     9181 tttgggaaga gaaaaactgg ccgtgcttat cagataaaga aaaataagag cgctgatttt
     9241 tttcattgtt agattgttaa actcataacc cttagaagtt tactggcgtg ggtgtgcttt
     9301 cgcttcgctt atcatcctcc acctctgttg ctaagtcaac ttttgaggtc atatctttgg
     9361 gtttttcaaa ctcttgtttt ggagtagatt gtctttctaa gacagactct cgcgtcacac
     9421 ctaattgttg atgaagtttg gcattcaccg ttaaatgatc cttttgttcg gttttgtgtt
     9481 gtggtgaatt ttctgatgcc gctttcatac cctcgcgaac aatggattta atctttcctt
     9541 cttctatctt gttattaatg gcatcaatag cggtaccaaa aagataagtg gccgcaaaag
     9601 taacgccagc aacaatggca gcaccggctg ccacaccaat tcccaatgcg gccacagcca
     9661 agcctgccgg agggaatagc aaagtaaggg cgactaatcc accagccgct agtaacccaa
     9721 gcccaagacc cgctttaatt tttgtaccca tatttttggt aaaagcgttt tcaggcccca
     9781 tttcttttac tttggcaaaa ttagcgatct taccgaaaag gccattatca gcgaaattgg
     9841 ctttatcctg aatttcaaac tttccgccgg aaacacccgt attggcagta tgatgaaggg
     9901 cctgtatagt agtaagttcc cgatcaagtt gttctacttt actgacttct ttaccatgtg
     9961 tcctgcgatg atccagaaat tcaccagtga ataacccgtc ctcactcatt gctgtccata
    10021 aagcattgcc aacaattttt tgcgccaatc tttggccacg atcattatta ctcttacatc
    10081 ctttcgtatt ggcgtaatcc agctcgccgg cgagagaagc cattaaggtt tgcagcttga
    10141 aattattttt gccatgatgc caggtgttct cataatagag ctcttgagct tgcatgaggt
    10201 aagcgcatga attgtaaaag tgctgcagtc tggatttggt ttcaggatct gtttctttat
    10261 tctggattac agggcttatt tgttctttaa gttcttgtaa agccttattg atttgccctt
    10321 tgtgttctgt gtgataagcc tttcttaagg catcgatttg attatgcagt tcataaatgg
    10381 ctgattcttg ttttttaatg cttttttcat agccatcaac ctgatttttc aattgatcca
    10441 gtttggcttg atcccatttc ccttcgcctt tctgatattc ttcgaaatag ctgaataatg
    10501 tttctcgata ggctcctttc acctctttta aggcattttc tttctcagtg atttgttgat
    10561 ttaggtcgct gacgtctggg ccttttaata acgtgtgtaa tgcgcctgct ttgtcataat
    10621 cactcaggtt ttgtgccact ttgtctaaat tgctgatgac gtcatcagtc atttggttta
    10681 caaaacgcgc attttgattt tttgtgacac tattgtcaga gagcggattg agcgctccaa
    10741 cctgtctaaa ccaattcact ccccaggaac tcatacgcgc ttgataacca acctctgttt
    10801 ccacttctgt attggagccg gtttgaatgg tgatttttgc tttggagcca tcaaaagcat
    10861 ccatcatttg ccgtacgtat tcaaattgtt ctttttgatt ttcggccgct accctttgcc
    10921 cggctgttac aacttgtgta taacattccg tgacagataa tggattatgt tctacataca
    10981 gctttattaa ttgtgctcgt gctttctccg catcaggagg aagctcatca gggcgatact
    11041 cttcaggaat tttattccaa aggtcttcat tggtcattcg gtcaatttga cgctgtgcca
    11101 gggtttggat gacttcttcc tggttgatta aggtgaccgc tttgatagcc agctttttat
    11161 agtcatcagc acccttaaaa tcagaatagg gcatgcgtgc gccaggacca gaaaaactgt
    11221 catggtgaat agtcacggtg ccatcagaat gccttgtccc ggcaatcatg cgcgccatac
    11281 tgctgttgcc cagaaccagg tcagagcgtt tatggctcgt agtgtcgtga tctcggttga
    11341 cttcggattc atccttgccc attagtcttt gttggcgttt ggtgtacctt ccacacggaa
    11401 tgtacataat aaattgttct tcgccttctt tagagttggg cgctttaaag cgatgtatca
    11461 aggtatcgcg attatgactg cgaatattat gcccttcttt atccagtgct tttaatttat
    11521 gacctgaggc ttttgcgaca gcctgtgtaa attgctgtaa actgccttcg actttggcca
    11581 actgttccag aaagtcttta ttagcagaac tttttttact tgaaaacaaa tccgcttttg
    11641 ctttgtcgga aggaatggtt tctgaaatgg actttttgaa agtttttgcc atttcatcaa
    11701 tggtattctt tctcgaatcc ttcttgtctt tattatcttt gtctatcaga gatttggcag
    11761 cggctacaag attgtctata taattattcg cctcttggat cacatcaggt tgccctgtaa
    11821 caggcttgcc atgtttatca tattgactgt tcaccaccac gacttgtttc ccgtccagca
    11881 tcgggtcttt gaatacctgt ctttcgccgg gataaccgta ggcaagatgc tgactttgag
    11941 aaggaacggg ttccagatca cttaataaat gcgtgacata ggtttctata ttttcagtga
    12001 ttcgtggcat ggtttattcc atatcataat atttatttta ttatagctgt caaacattaa
    12061 cgtaagatta ataagttctt gcattgagtg attgcgtagg gcttcactta aagactggtc
    12121 tgaaaaatag aatttagtac tattctcaga aaatagtgaa gcctgataat ttcctagtaa
    12181 aactcaatag tggtggtagt cacatccaaa ggctccggat catcgacatc taaaatgaca
    12241 ggctcaacca ctggagcggc aactgtcact tgttggtatt caactacttc tgctgtagta
    12301 caacagcaac ctgcagttgt tataataaca acgatcagca ttatccattt catagcgaaa
    12361 cctctcgtca taacttaacc aaattgtaga ttatttgttc atatattgcc aatgttttat
    12421 ggatgccttt gcgtgtttgt tcatctgaag aagttcaaat tcgtttgaaa tgggtttatt
    12481 ctttggagtg ctttccatta atgtaataaa ggaatgccaa aatttgggct acggcaatat
    12541 aaagtgcttt gggtatttcc tcattgaggc gaacttgaga taagagcgct gttaattcgg
    12601 gattttgttc gagaggaatg ccatgttctt tggctgtatt gataatttgc tgagcaaata
    12661 aaccttctcc tttagcggtt actttgggag gagaattgcc atcataacgt aatgcaacag
    12721 cgacgggttt ctttcttttc atatttttat atccaataaa cgaaggtttg tggcatcgat
    12781 atgattttct tccaacccaa cttgcagctt ccattctctt aagtgaagac ctgattcata
    12841 gagcgtttct tccaaatcct tatggtaatc attgaggagt tgcaatgttg acgattgctg
    12901 agtattaacc ttgatatcga tgtttttagc gtaaatcgaa accgtagctt gtatgcttcc
    12961 taaatgggat aaattcaaag cgaacgatat tgaccattta gacagttgca taggttctgc
    13021 cttgtgttgt ttgatcataa tagggattac atcaatgcca tcgggggttt ttaaaggaaa
    13081 atcaagcatg attagatagg cttcttgatt ctcctgcgat aaataattaa tttgatttgt
    13141 cgtaattctc gctaatgcct gtgttacctg ctcacgtaaa atatggtgta ccgcctctgc
    13201 tgatagtcca ataagattca atatcgtgtc tttagtcagt ggctgaggga tagctcctgg
    13261 taaaggaagg ggcgcttgct gaagaaaacc tgattccaat tcgttcggca gattgattgg
    13321 gagtttactg tctgtgccta aactgtgtaa taatttaaaa cacaaccctt taaaatccga
    13381 ttgtatttct cggggtatct ttccttttaa caatttggat tctaagaaca caccactttg
    13441 actaatcgct tgcataagtt gattaggtaa ttgagtcaaa acagggacat tgcctaaaat
    13501 ggctttcata acctgattga tgggagccga taaattattt tctgccatca attgagtcag
    13561 tgtctgtaaa agattggtag ctggtgcttg ttttggtaaa tactgaagta acgcattttg
    13621 caatagagat ggaagcggtg ttttttgttg cacttcaagg atggtttcgg ggtggttcgc
    13681 tatgactttt acttcaaata actctcccgg tgtaaaatga tgagatgcct ttgcgttgag
    13741 attttgccca ttgatattga taagaacctg atcattagac agtgttgtga caacaaccgt
    13801 tttcagaatt tgtcctacat aaagttctgt cgccggtttt gctaatttga tttcttgtcc
    13861 aggagggaat ctgacactgg aggttttgct catctcgacc gacatacacc atccttatca
    13921 tcagttagac tgctttttct tatcgtcgtc ccccgctgga ctgtttcgta agttgtctaa
    13981 taagtatata ctcatcgtct taattatcgg cagaattatg aatttcttta tagttcgttg
    14041 ggaaaggata ccaggcatgg atataagagt aatcattaca caaaatgtaa ttgaaatagc
    14101 cttattcatc tggtcattgc gcaatttaaa cagggtgtgg tgatgttaga tagagcaagt
    14161 gtagtggatg aacaatttct gaaaagagtg ctgcaagggg atttcccaca attaagaagc
    14221 agcatgagcc ccgatacagc agagttggac aagaaaactg ctatagaatt ttttgactca
    14281 caaattaaat ctcggcttct tgatattatt gcgagacaat taaaggaaaa aggcctctct
    14341 ttttatacga taggaagtag cggccatgaa ggcaatgcgg tcttgggtca ggtttttaga
    14401 gcgagtgata tggcgttttt gcactatcgc agcggtgcct ttttcttgca acgagcaaag
    14461 caattacctg gaatcgatgg agtaaaggat attttattgt cattagtggc tgcggcaaat
    14521 gatcctatct cagggggcag gcataaagtc tttggcagcg tccctttaaa tatcccgcct
    14581 caaacgtcga ctatcgcatc tcatttacca aaagctttag gtgctgcttt atcaattacc
    14641 cgggcgagag agttaggatt gccaagtaaa ctggcgcctg attccgtgat actttgttcc
    14701 tttggtgatg cttcaaccaa ccatgcaacg agccaagcca cattgaatgc ttgctcttgg
    14761 ataacccagc aaaattatcc gctaccgatt gtgttcattt gtgaagataa tggaatcggt
    14821 atttcagtgc ctactccatc cagttggatt aagtcatccg tctcctctcg tccaggaatc
    14881 cattacattg agtgtgatgg attaaacatt gctgatgttt ttgccaaagc gcaggaagcg
    14941 gactatttag caagagtaaa aaaacaaccc gtgttcttgc acatgcgttg tgtccgtttg
    15001 ttgggccatg ccggttctga tattgaatcg caatacaaca ctcaggaaga aattgagcaa
    15061 cgagaagcaa atgacccttt attgcataca gcaggaattt tgtacaaaga gggttggatg
    15121 agtttgcagg caatggttga gctttatcag gacaaccgag ctttaataga agcaaaagca
    15181 ctggaggcaa taagagaacc cagaatgagt tctgctgaag aaataatggc ttcaattgtt
    15241 ccacgagttg agaaaaagcc aaagtaccca ttgccagatg ataatagacg agagcaagtg
    15301 tttgccggag cttataatca actcaccctg aagcgtaatt tatgccagca gatcaatttt
    15361 gctttgaccg atctgatgat gcagtatcca aatatgttga tttttggaga agacgtagga
    15421 aagaaaggtg gagtttatcg agttacggct gatttacagg ctcgttttgg gcaacgcagg
    15481 gtttttgata cccttctcga tgaaacgacc attataggta ccgctattgg tttagcacat
    15541 aatggcttta ttcctgttcc ggaaattcag ttcttagctt atctgcataa tgccgaagat
    15601 caattacgtg gagaagcatc cactttatct tttttttcaa gtggccaata tcaaaatcct
    15661 atggtacttc gaatcgcctc tcttgcttat cagaagggat ttggcggtca ttttcataac
    15721 gataattcga tagccgtttt acgcgatttg ccaggggtta tagtggcctg cccctctaat
    15781 ggtcccgacg cagcaaggat gttgaggact tgtatgcgcc ttgcctatga ggaaggacgg
    15841 gttgttgtgt ttttagaacc catcgccctc tacatgacca aggatttgta tagcccggga
    15901 gataatgggt ggttatttga atacccgtca cccgatgaaa tgatttctca aggagaggtt
    15961 ggcgtatatg gggaggggga tactgttatt ttaacatatg ccaatggata ttatttatca
    16021 cgccaggcgg aaaaggtttt gcgtgaagca cataatattt ctgttaagat cgttgatttg
    16081 cgttggctca gccccttgcc aaaagacgcc attttaaaag aaattgccaa agcaaaacgt
    16141 attttaattg ttgatgaggg tagacaaagc ggctcaatta gtgaaggatt gatgacatta
    16201 ttgatggaag aggcttcgcc acggttaaaa ataaagcgga ttacgggcaa agattgcttc
    16261 ataccattag gaactgcttg gcagtattta ttgccaagtc aggaaagtat tatagacgcg
    16321 gtaatcgcat tacagtcaga taaaagggag aaggaaagtg gacgacttgt tgtttcttga
    16381 tgaacaatta catgatgacg agcgcatgat acgagatagt gtgtctcgtt ttgttagcaa
    16441 tgacgtaata cctttgatgg ctgaatctta tgaacatgcc cattttccaa aagagttgat
    16501 aaaaaaatct gctgatctgg gtttgttggg cttaacttta cctgcggaat atgggggtgc
    16561 agaagcttct tatgttgcct atggtttagt atgccaggaa ttggagcgag gtgatagcgg
    16621 tttgcgcagc tttgtttctg tgcaaagttc tttgtgtatg tatcctattt ttaaatttgg
    16681 cagtgaagag caaaaaaaac gctacctgcc agagatggct gcggggaata ttataggttg
    16741 ctttggatta actgagccag actcaggatc tgacccggcc agcatgcgta ctcatgccaa
    16801 aaaaattgaa ggtggctggg ttttgaatgg tgctaaaatg tggatcacca atgcccctat
    16861 cgcagacata gcgatagtgt gggcaaaaac agaggcggga attcgtggtt ttattgtcga
    16921 tacaaaatct gaaggcatga gttgtcctga aattaagtta aagatgtctt tacgagcctc
    16981 cattaccggg gagctggtct ttgataatgt ttttgttcca gacgagaatt tattgccaaa
    17041 aagtgaaaaa gggcttggag ctgcgttaag ttgcttgagc caggcgcgtt atggtatcgc
    17101 atggggcgca atgggcgctg ccatggcttg ctttgatatc gctcggaatt atttgctgga
    17161 acgcaagcag tttgccaaac ctttggcctc gtttcagttg attcaaaaag atttggctga
    17221 tatgtataca gaaattatta aagcacaatg ccttaatttg cagattgggc gtttaaagga
    17281 tcagcatcgt gagacaccga ctatgatttc tctggctaag ggtaatgcgt gccgtgaagc
    17341 attgaaaatt gcgagaaaat gtaggaatct aatgggcggg aatggaatta gtcttgagta
    17401 tcatgtcatt cggcatatgc tgaatctgga gtcggtcttt acttatgaag gtaccgataa
    17461 tgtgcatact ttggtcttgg gaagacatat aacgggaatt aatgcttttg gttaaggatt
    17521 tttctgcttt tatcgagtta gttctgaata ccacaagata actattctta atcagtaatg
    17581 attacggttc aagttccttt ttcttatgga aagaggaact gcttaaacct ttttgatttt
    17641 agagttgagc gatcgcccgc cagattatgg tgctgaatta ggaaggtagg gccctggcat
    17701 gggttatttt atggatattc gatgtaaaac ggttttatac aaacaattac aggagtttaa
    17761 tatgattaac atgaagttaa aagcaacttt gatagcctgt ttgtccgttc taactttaag
    17821 cagttatgca aattcatcag aaaataaaga agccatttta caacagtgtc atgacttggc
    17881 ttcaacagta gcctctttgg tttccagcca ggctaaaaaa acctgtgcgg aaaaattggt
    17941 tattgcttca ttacacattg atacagcggc ggactggatt gtagaggatg ttcattctgc
    18001 agcaaaacaa gaattagata atgccattta ttctttgcaa tatgctgagc tgaatagttg
    18061 taatcgctat atacaaatat ctcattccaa actggaagca caaagaataa aaagcctatt
    18121 atagaaatag aaaataatgg ttatattttt tgaaaaaagg tctctttgct tatagggcaa
    18181 tgagacttta tgtccaactg aagcagcagt ttagcctgtc attaactcca tatcacctaa
    18241 tggttttttg agtcttctcc cggcaagggg cagaaggtat tcggtaaact aatccttgcc
    18301 aaatttctta tctttctatc cctgctacac ttaaatatag agaagattat atttaagggc
    18361 agcaatatga ttgagttaga gccagaacca gacacagtcc tggatcctaa agatattttg
    18421 actgaggaaa tgaagttgga agggtcagat aatgatatgg atcataatca attcactcgt
    18481 ggtgatgaag tggatttagt cggtgatggt gttcctggat atgaaacaac aggacaagat
    18541 ggaacagatg atgaaattga agagcaaaga gaagctcgct atactcctac cttgaaccct
    18601 aatcccaaaa atgtgccttg atctccgtaa tgaggttcaa ggtgtagttt attgtaaatt
    18661 ttttgcaaaa ttcaccaagt tgccaaatga aacattcatt ttaaattatc atctgcctgt
    18721 tagtaactcc aaggtttaaa ataatggttg tggcaaagaa aagagaagag acgcaaaaag
    18781 atgaacgtgc acgtgcaatt gaaaataatc cctgcagtat ctgtcgttca ttaggattac
    18841 ctttttgtcg tggtcatggc ggcggttctg ggggtggcgg atcggatagc gcttctgacg
    18901 acaaagcgga tgataatgat catcaattgc tcgcatcaaa gaatcctgcc aatgttacaa
    18961 atatagagca actactattg catagcggcc tatggcattt gccagaggat gaagattttg
    19021 tttttgaatt taaagacccc cttgctttag tcaatatgac tctgaacatg gagcttggtt
    19081 tattggtgtt gcaaggcaag cgcgagcttg gcaatgaaga gcagaatgct ctggatgagt
    19141 tgtatgagac gattgcacag gaatttaacc tttttaaaaa ttcaataaaa gacccacagg
    19201 ttcctctgga aacaatgacg ctcgccagag agggtaatag tttgactata aagataccca
    19261 gccctaaata ttatgatttg tttgtgcaac agttgatgga aaaaaatttg ctacccggca
    19321 acgcaccagt tttgcaacat caaaaccaat cagtgaaaca tccggaaaaa tcggaagcgg
    19381 cgaaggattc agtaatggaa gaatataagt catccttgcc taatccattt gatattagcc
    19441 gcggccctaa attacctgat tggatataat ctttcccagg taaggatgag cggaaaaatt
    19501 tctccatcat gctattctta attaaagtga gttatggata aggattatat aagaagcagt
    19561 cgatatccag gagccgttca ggctgaggga tgcataagca tcgtctcgaa gccttagcac
    19621 gcaacattaa attccataca aggcttcgag acagcataaa tgcctcatca gcccgaacga
    19681 ttggagataa cgcctatctt atccataact catgttaatt aaagagatca gcatgatcct
    19741 gaaatttttc caacgccagg aatgacatag tatgacaacg cccgaaatta ccttatccag
    19801 agtgtactct ccaccggagc ataaagaagg gctatggata cttgtagacc gattatggcc
    19861 tcggggaata aaaaaagaga gtattcctct ggatatgtgg ttgaaagaca tagccccaag
    19921 cccggctttg aggaaatggt ttcatcaaga cagtgagcat tggcatgagt ttgcaaagca
    19981 gtatgcccaa gagttaaaag agaaacccga attgatagaa cacattttgg cgttagcgag
    20041 cacttcacca gtcactttat tttatgcggc aaaagatctt caacataatc atgcgctggt
    20101 acttcagaaa gttctaaaat cgtggccaaa aataccagaa atcaagtagg gcctatttgt
    20161 gcgaaataaa actcagacaa gccagtaaac ctaaacactt cagtcattat agtaaactat
    20221 aaccccagga cttacgaaaa accccaatct atatgttaaa aaatgttttt aattcggtca
    20281 tttagtttat ctaaactccc tcattaaaaa tattttttgc cttgaccatg gagtttttcg
    20341 taagttctgc ccttgttatt ttagccttat ttagatatca tgcctaacca atcggggcgt
    20401 agcgcagcct ggtagcgcac tagcatgggg tgctagtggt cgaaggttca aatccttccg
    20461 tcccgaccat caaatcccct ttaaaatcag taagttgtat gttcttggtt aaatccttta
    20521 aaaatcaaaa attctgctag gtgt
//
LOCUS       DATNSN010000041        20482 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.041, whole genome shotgun sequence.
ACCESSION   DATNSN010000041 DATNSN010000000
VERSION     DATNSN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20482
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.041"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..141
                     /locus_tag="VAH76_001262"
     CDS             <1..141
                     /locus_tag="VAH76_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385903.1"
                     /translation="YNNSSPAAQLQNAYLRENDNLIIKSTIVLSKELGLNFAAGGVET
                     EE"
     gene            293..1964
                     /locus_tag="VAH76_001263"
                     /pseudo
     CDS             293..1964
                     /locus_tag="VAH76_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid synthase"
     gene            complement(2031..2107)
                     /locus_tag="VAH76_001264"
     tRNA            complement(2031..2107)
                     /locus_tag="VAH76_001264"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2071..2073),aa:Arg,seq:tcg)
     gene            complement(2168..2243)
                     /locus_tag="VAH76_001265"
     tRNA            complement(2168..2243)
                     /locus_tag="VAH76_001265"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2208..2210),aa:Lys,seq:ttt)
     gene            complement(2299..2374)
                     /locus_tag="VAH76_001266"
     tRNA            complement(2299..2374)
                     /locus_tag="VAH76_001266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2339..2341),aa:Lys,seq:ttt)
     gene            complement(2461..3339)
                     /locus_tag="VAH76_001267"
     CDS             complement(2461..3339)
                     /locus_tag="VAH76_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385904.1"
                     /translation="MKWQIPAKTFLLGEYAAIAGEPAILLTTSPCFTLTLTENAKLEG
                     IHPESPAGLWWQKNQCHPFGLSWQDPYHGQGGLGASSAQFLACYLATCSLQEITPRLN
                     HMLEAYYQSSWSGLGLRPSGYDVIAQSQQGCVYIDKQKNQVNSFSWPFKDLSFLIIHT
                     GNKLATHHHLQEVTLPSQIDYLASLVIKAKEAFDLAHSHHLIQCINLYAQKLTELNLV
                     SQHSLPLIQSIKFYPETLAIKGCGALGADTLLIITDKRDLQSLKDKVQDQNWLILATE
                     DNLTVRNNNSLFEKSI"
     gene            complement(3336..4634)
                     /locus_tag="VAH76_001268"
     CDS             complement(3336..4634)
                     /locus_tag="VAH76_001268"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO1385905.1"
                     /translation="MSIAANAGELFRGFSKLSREERFQRLCALGALTHEDITFLKQGG
                     IKDLNLADKLIENVIGYFQLPLGVATNFNIDGRDYVIPLAVEETSIIAALSKSAKWIR
                     QHGEINTWVHGECILGQIQLAKVKDFHRFSDLFNKNRQYFIEIANKDVAANMVKRGGG
                     VTDLQVRHLKREDGQDMAVIHLTMNSCDAMGANIINQVLEYLKQPIEQITGEEVTMCI
                     LSNLNDQKLTTAQVIIRNIDPILGQKLQEASLFAEIDPYRAATHNKGVMNGIDPVLIA
                     TGNDWRAVEAGIHSYAARSGQYKAITRWRYQNEILTGELTAPIIVGTVGGVTSLHPTA
                     KMCLRMMDITSANQLSQVIAAVGLVQNLGALKALCTDGIIQGHMKLHIDNLLLVAGAN
                     ENEMPVLKEKLQEWLNLNKRVSLNNAYDLLAEIRQAPVAV"
     gene            complement(4625..5653)
                     /locus_tag="VAH76_001269"
     CDS             complement(4625..5653)
                     /locus_tag="VAH76_001269"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO1385906.1"
                     /translation="MQNEYSQFEQRKRDHIELALMPANQSSELNPFDHFSLVHEALPD
                     LDFKDISIQSIRLKKTVEKPFIISSMTAGHSNALEINYRLMEACSKTKWAMGVGSQRR
                     ELTDKQAAFEWAPLRRDFPMVSLFSNLGIAQLIDTPISAIQRLIDTLQAEALIVHCNP
                     LQECIQPEGTTNFQGCWTALEALVKKIASPVIIKETGCGFSKNTLLRLNNIGVAAVDV
                     SGVGGTHWGRIEGHRANKDPIRHRTADTFRNWGIDTLQSIRNAISLNPSFEIWGSGGV
                     RNGLDAAKLFALGATTVGFAKPMLEAALDSTGQVLTQMNTIEYELKTAMFCTGSRVLD
                     DLKEKACP"
     gene            5821..6879
                     /locus_tag="VAH76_001270"
     CDS             5821..6879
                     /locus_tag="VAH76_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2050 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385907.1"
                     /translation="MKSKLEYHFSANADGDESCIEDVDGNEFTPNGSPSHGWTAWTRF
                     FGHKNQSPKYVVKQPRPDKVRSLSDLRDKKLNNEWNRTRYQHELDIWNLVYPDNKAYL
                     FTDSGIRLVLPYLPGKTLFSFTSREEDQLTWCQLNHAVFCEFKRFTELGLKHCDLKYD
                     NFLIERKQDGTFKAYIIDLDSVGKLKPNDQDYDWNIVLASLFDDTGLKSPDVCPPGYI
                     ALKSIDVPAENENEARSKLDKIKKELSQKKPTLIRIKNCQQNKLDFYFVYGCLYIGQW
                     GFTKLDEKIISSVNLSNFSGSLVDCRAASVIGNHIKLKQGHISYRLDLYINCLNEKIA
                     ELTLLKSQNSPKKLYTPF"
     gene            complement(7017..7316)
                     /locus_tag="VAH76_001271"
     CDS             complement(7017..7316)
                     /locus_tag="VAH76_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385908.1"
                     /translation="MGRFIFKGTSLIKLMLAFTLLIGALILVTPANAKAVNRDINPII
                     NKANPTQMAYWVRYRYWGPYYYRGYYYRPYYYRWGYPYYRPYYRPYYRPYYYYRW"
     gene            complement(7442..8266)
                     /locus_tag="VAH76_001272"
     CDS             complement(7442..8266)
                     /locus_tag="VAH76_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385909.1"
                     /translation="MNEKLIETQSNLIKQAEAELTQSNQLLESLQLEKGLPEESDLDE
                     LDSLKSLILVLQYRLDTQNALSQLGHLQQYLLQLLGIEAKHSFEFNLERLAFALGTDE
                     LKQILAMLHHLVDSLLRVIAQKQLKESHDKRKSLEQTKQFKPIDTLVKVLNKQKLFMA
                     KLDELKLVLENIGGAPQPGIVYDHIAALQGPISRFYQALQHGLGLSNSLYQQLQKKNQ
                     LSYQLPDTLLKPEHVIRNMNFHPESQRLFKTTLEHNKIENLEERAAAKRLGHFFNH"
     gene            8387..9511
                     /locus_tag="VAH76_001273"
     CDS             8387..9511
                     /locus_tag="VAH76_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385910.1"
                     /translation="MEQTSAQIVFEQKNATYQCCGSWSVLTLDGLPKKFSTSPLPDSQ
                     QITINGEKISVFDSAGALALKKCIDELKKRNNQVELVNFSDSQHQMLELIESKQEILS
                     YQLPIVSKEGVLYQIGKESENKLHQVDGLIILIGDLSTRLFEAFAYWRRFHLPSIVSV
                     IDSAGLKALPIIGLLSFLIGVVLAYQMGLQLETYGANIFIAYLSGMAIFREFGPLITA
                     IIVAGRTSSSFTAQIGSMKINEEVDAILTMGLSPTELLVLPKVLGLLIVFPLLIFWAD
                     VFSIWGAMIMSKNMLNIGFEDFLTRLRDSVGLNQMMLGLYKAPAFSILIALVGCFQGF
                     RVESSADSIGSQTTKSVVQALFLIIIADAAFSIAYSWMGL"
     gene            9512..10249
                     /locus_tag="VAH76_001274"
     CDS             9512..10249
                     /locus_tag="VAH76_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO1385911.1"
                     /translation="MSEPVIEIKGMKNCLGGQWVHTDVNLTVERGEILAIIGGSGSGK
                     TTILRSLLMLLKPSAGSIKVFGEEIYNLDTEQAFRLRRRWGMLFQHSALFSAMTVLEN
                     VMFPMREFTTLDQEFMQELALLKITLVGLSKEAAVKYPSELSGGMQRRAAAARAIAMD
                     PELLFLDEPTTGLDPRGARLFDDLVVFLRDALNLTIVMVSHDIESLKRTTDRVAFVGD
                     GKILSVEPINELMKNKNPLIAEYFSKF"
     gene            10279..11202
                     /locus_tag="VAH76_001275"
     CDS             10279..11202
                     /locus_tag="VAH76_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEO1385912.1"
                     /translation="MESKTNYTIVGLIVLILTAGLLSAGLWLSVGFNQKEYTSYTVYL
                     KESVSGLSVESPVKFNGVQVGYVKEIKLNKNDPRQVELLLNIEKSTPITTSTSATLIS
                     QGITGVTYVGLSAGSSELTPLRKMPGEPYPVIPSKPSLLNQLDAVLKEVAENVGAVSE
                     KAQLIFNEENADNVRKSLANIERITEIIADKGQTIDSSLNNLDVFVANMAKTSKQFPQ
                     LIKDLKTGISKFKSLADNMSAAGKDVSKTMIAGKNTIDQISQQAIPPAVILLRRLNAI
                     SANLEKVSNEMRQNPSVIIRGTKTPKLGPGE"
     gene            11206..11808
                     /locus_tag="VAH76_001276"
     CDS             11206..11808
                     /locus_tag="VAH76_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEO1385913.1"
                     /translation="MRVLSSVCFILGILTLMSCSPVKVPVTNEYQLTAYSTKQLTRKP
                     RPITIQVTVPEAVAGYQTEEMLYMKKPFKLEPFVKNAWTSPPADMLFPLLVQSLQSSG
                     YFYAVTSSPYSEEADYRLDTQLLKLEQNFIKKPSVLEFSAKIVLTHISDNQIIGSRIV
                     SLQIPCSQDTPYGGVIAANQATFRFTATATDFVVSHIKRD"
     gene            11906..12436
                     /gene="pal"
                     /locus_tag="VAH76_001277"
     CDS             11906..12436
                     /gene="pal"
                     /locus_tag="VAH76_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEO1385914.1"
                     /translation="MKAGSFYKLGLLVASAVLVAACSKTPGSADGGAAVGDGDATAQG
                     LGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARV
                     MIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEA
                     SHAQNRRVEFIYEATR"
     gene            12433..13401
                     /gene="ybgF"
                     /locus_tag="VAH76_001278"
     CDS             12433..13401
                     /gene="ybgF"
                     /locus_tag="VAH76_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEO1385915.1"
                     /translation="MINCKKSIITLCFVLMLPFALWAEAPVIDDSENFAMIDRQEEYD
                     APLVNPKYDDPQIENAELDGPQNDNYSTDTSQSYDEPALVKEDRSNISDNAKLIDKIQ
                     QLQKEIQELRGQLEVQAHDLKLLQQQQVAFYKDLDSRLSNSSTSAKTVQNDKPATDIS
                     LGSNSPETLKVASPQIKAGSSNSKPQPVVAVSRANPADEQISYLAAYELVKNKRYDEA
                     IKSMQIFVQKYPRGGYTANAEYWLGELYLVKKDYSKAIEHFEIVLQQYPSSSKAAASL
                     LKSGYAYAEKGDKQEAKKRFQQVVKTYPDTPTAQLASSKLEAINAL"
     gene            13398..14051
                     /gene="queE"
                     /locus_tag="VAH76_001279"
     CDS             13398..14051
                     /gene="queE"
                     /locus_tag="VAH76_001279"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEO1385916.1"
                     /translation="MKRFNEQLRITEIFHSLQGESVTVGLPTVFVRLTGCPLRCQYCD
                     TAYAFSGGEVVEIDDILNKVASYQCQHVCVTGGEPLAQPGCIPLLSKLCDAGYSVSLE
                     TSGARDIASVDQRVMIVMDLKTPDSREADKNLLSNLSFLKPTDQIKFVLCSRNDYEWA
                     CSMLSEYQLAERVQLLFSPSWNQLNPADLANWIIQDKLPVRFQLQLHKILWNDAPGH"
     gene            14069..15016
                     /locus_tag="VAH76_001280"
     CDS             14069..15016
                     /locus_tag="VAH76_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO1385917.1"
                     /translation="MHWFAQAPSNIALIKYMGKKDENSNLPDNSSLSYTLSNLLSSVK
                     LEKLPTKKDIWEPLSIPGAPEFNLSVEAQKRFIDHLVRLKEYFGYVGGFLIQSSNNFP
                     HSSGLASSASSFAALTKCASIALSDLTQKPLPSIDEQAQLSRLGSGSSCRSFYAPWAL
                     WTGDKVSAIDLPYKDLLHQVIVISSQEKEIPSRVAHKLVKTSPFYETRSERAEANLKL
                     LLNAFENKDWTSIYQICWHEFLDMHQLFKTCEKPFSYITDNTLHILSVIEKFWNEKGD
                     GPVVTMDAGPNVHLLYRSDQTDLARQFKSDHLVGNYDVL"
     gene            15006..15884
                     /locus_tag="VAH76_001281"
     CDS             15006..15884
                     /locus_tag="VAH76_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO1385918.1"
                     /translation="MFYDFETTTFGKWILAGEHAVVRGHEALVFPIKERQLTLKYNPS
                     SPSLSAEFQGINGSDMHLLFWSVLERGMHLLGRSLNELGGHFYLETNIPVGVGMGASA
                     ALCVAMSRWYCAQQMIQDNQCNEFAKQLEHLFHGKSSGLDIAGVASDTGVYFKSGLCT
                     PVKQTIYPYWYLSSCNQIGITSHCIQQVEYLWNKNPELAHQVDKQMVEAVQEAKAVLE
                     EGGSNALNRLAKAINKAADCFLQWGLVSESLQQHMNHLFADGAIAVKPTGSGGGGFVL
                     SLWDKIPSSHTELIPV"
     gene            complement(15897..16166)
                     /gene="ftsB"
                     /locus_tag="VAH76_001282"
     CDS             complement(15897..16166)
                     /gene="ftsB"
                     /locus_tag="VAH76_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEO1385919.1"
                     /translation="MRPIFIILIVALVALQHKLWLGDGNIIQWIKLEKKLEAHKSQND
                     KLAARNKALEADIKELKSGDQALEEQARYELGMIKQNEVYYQFVD"
     gene            complement(16388..17656)
                     /gene="eno"
                     /locus_tag="VAH76_001283"
     CDS             complement(16388..17656)
                     /gene="eno"
                     /locus_tag="VAH76_001283"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO1385920.1"
                     /translation="MHIHKIQAREILDSRGNPTIEADVILTTGIIGRASVPSGASTGS
                     REACELRDNDPKRYAGKGVQKAVKHVNNEINQALQGLSVEDQENLDRILCQLDNTENK
                     SHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
                     IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQAL
                     DLLSEAIEKAGFRLGEDIVFALDVAASELFNEGFYHMYSENQKFDSHQLIEYYANLIS
                     SYPIVSIEDGLDEKDWSGWKQLTTHLGNKVQLVGDDLFVTNPKILREGIAQGIANAIL
                     IKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLC
                     RTDRTAKYNQLLRINELASLPYAGKNILKR"
     gene            complement(17688..18290)
                     /gene="maf"
                     /locus_tag="VAH76_001284"
     CDS             complement(17688..18290)
                     /gene="maf"
                     /locus_tag="VAH76_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEO1385921.1"
                     /translation="MSKFLQQKPIILASSSTIRHKLMKSLGLDFLVVPSNCNEEEIKT
                     RHKSDELVELGITLAKIKALDVSQHYPEHYIIAADQLCVAGKRVFNKPQNHQTAVSHL
                     RELSGKQHQQIACLCIVKESKILWQYHETATLTLHHLSEKTIEAYLQAEKPYQSCGAY
                     QYEGLGKWLFKEVQGSEDTILGLPLMPLVNALVNLKVVGI"
     gene            complement(18283..19041)
                     /locus_tag="VAH76_001285"
     CDS             complement(18283..19041)
                     /locus_tag="VAH76_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEO1385922.1"
                     /translation="MLSATSLKIIFAVSIFIVILIAGWYPFKKRLKDDKHIDFPIGET
                     LATGVFLGAALLHMLPESNTLFKEMGYNYPFAFIITGVVFLIFLWFEHLGKELYHHHD
                     AEHPAFAILAWAMLSVHSLMLGAALGFTQYNSMIIMLFLAIITHKWAESLAIAIQLNK
                     SSMSTRKSMVFFFFFSLMTPLGIYFGWYFGHGVETHSLFDPILIAASAGTFLYLGTLH
                     GLERCVMVERCCNLSDFSFVIIGFLLMASVAIYV"
     gene            complement(19157..20299)
                     /locus_tag="VAH76_001286"
     CDS             complement(19157..20299)
                     /locus_tag="VAH76_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO1385923.1"
                     /translation="MYPHDRYWQAKKVTLIGAFINALLGVVKMIGGFLYHSHALIADG
                     VHSISDLITDVMVVFASKYGSVSADDSHPYGHQRIETAATLLLSLLLILAGCGIAWDA
                     LDELLKSDHTMPNWLSIPIVCFSIIANEALFHYTRHVGKKISSQLITANAWHHRSDAA
                     SSIVVLIGLIGSLAGLVYLDAIAAVIVSLMIIKMGWSYGWNSVKELVDTAVDAELFSQ
                     IERVIQSIDGVKRIHQLRSRFMGSDVLIDVHILVSPKISVSEGHYIAQHVHHTLVKEI
                     DCVKDVIVHVDPEDDEISCPSLHLPNRAILQEKFFNDIRHDFPQILFWNIHYLNGKMS
                     LDIFCSNDFNQWKKLHNRVTSTLKSQIDIKEVRLFGLHEEMHHPSN"
BASE COUNT         6120 a         3837 c         4214 g         6311 t
ORIGIN      
        1 tacaataata gttcacctgc tgctcaacta caaaatgctt atctcagaga aaacgataat
       61 ttaattatta aatcgacgat tgttttgtct aaagagttag gattgaattt tgcggcaggg
      121 ggtgtagaaa cagaagaata actcaatttt ttaagtaaat ctccaagaag tcattctttc
      181 gcttttcgat tctttaagaa gaggctcctt acatgacatt gatgcgctaa atcttgtgct
      241 atgctgtatt atatcttgtt gattattggg cgtaatgccc aggatgtcag ccatgaaggg
      301 gctagaaaaa caatgctgct ctaaggaaag gatactcact cagagccaag aattgcaaga
      361 aggggtggat ttctcttgct gtgtacccgt tgagagtact tcctccaata gcttcttttc
      421 gttttttact cgattaactc aagctaactt ggctaaatgg acagcaggaa taagtcctgc
      481 tgccatcggc tcttcctatt ccacctggct ttggcaatta gctcaatccc ccggggtctt
      541 atgggaactc gctttttatc cggttttgca cgccaaggat tgcattaata atatcgtttg
      601 cgttgagcgt gctgcggatg gtaaagacgt gcgttttaaa aagaacagtt ggcagccaat
      661 gccttgatgc cctgtcacct tccaattttg cttggtctaa tcctgattta ttccatgaaa
      721 ctatgcgcac tgggggactt aatcttatcc aaggcaccca aatcgcgttg gaggattggt
      781 tggaaaagct ggcgggtgca ccgcctacgg gctctgaaca ttttatccca ggtcaacacg
      841 tggccattac accaggacga gtggtgtttc aaaatcattt gattgagctc attcaatacg
      901 aatctcatac taagacggtg tataaagagc ctatcttaac ctttcccgcc tggattatga
      961 aatattatat tttggatttg tcaccgcata actccatggt gaagtggctc gtaagtcagg
     1021 gacacaccgt attcatcatt tcttggcgca atcctgacaa agaggaccgt gatttaggga
     1081 tggatgatta ttataggcaa ggagctatgg ccgccattga tacggtatcg actcttttgc
     1141 caggaaccaa aatcaatctg atgggttatt gtttaggtgg caccttggct atgattactg
     1201 catcggcgat gggaagagac aaggatgaac gtttaaacag cttgacactc ttggcagctc
     1261 aaggagattt tactgaggcc ggggaattaa tgctttttgt gactgaaagt caagttgact
     1321 ttcttaaaag tatgatgagg gaacaagggt atctcgatac caagcaaatg gcaggttctt
     1381 ttcagatgtt gcgagcctac gatttaattt ggtcaaaaat ggttcaggat tacatgcatg
     1441 gaatgcggcg agggatgatt gatttgacgg cttggaatgc ggatgccacg cggatgcctt
     1501 acaaaatgca cagtgaatat cttgaaaaac tatttttaag gaatgatttt gctgaagggc
     1561 gttatactgt agaaggaaag ccagtggctg ctgagaatat cgagcggcct gtttttgcag
     1621 tgagcactga aaaagaccat gtagcgccat ggcaatcagt atataaaata catcttatgg
     1681 tgaaaggaga tgtaaccttt gttctcactg acggtgggca taatgcaggc atcatcagtg
     1741 aaccgggaca tctcgggcgc tcttatcatg ttcatgagca tacacaaggc gagacgtact
     1801 taaatcctga aagctggctt gcgatggcaa aggtacgaga aggctcctgg tggcaagaat
     1861 ggcatgagtg gttagtacga caaaactcta aaaaactcat tccgccacct gttatggatc
     1921 agtcactgcc agcagctcca ggaacttatg tgcttcagaa ataggactca tgttaggggg
     1981 ttatgttgtt aaatggtgag tgcccccgta attgggaaag agccattaaa tggcgcgctc
     2041 ggagggactc gaacccccga caccttggtt cgaagccaag tactctatcc agctgagcta
     2101 cgagcgcaat ggggtgtgga tgatcatttt atatttctgg tctttttatg caggagggag
     2161 accagaatgg tgggccgtat aggattcgaa cctatgacac agaggttaaa agcctcctgc
     2221 tctaccacct gagctaacgg ccctttcagg gtataaccag caaaatacaa tagttgatgg
     2281 tataggttta ttaaaaactg gtgggccgta taggattcga acctatgaca cagaggttaa
     2341 aagcctcctg ctctaccacc tgagctaacg gccctaaaga ggctgaaatc ataccggatt
     2401 agagcagaat ttcaagtctt tttagagatt attttttgtt taacaaacaa gatgtgttat
     2461 ttatatgctt ttttcaaaca aggaattatt attcctgaca gttagattat cttctgtagc
     2521 taaaattaac cagttttgat cttgaacttt atctttgaga gattgtaagt ctcttttatc
     2581 ggtaataatt aacaaagtat cagctcctaa agcaccacag ccttttattg ccaaggtttc
     2641 cggataaaat tttatagact gaataagagg cagactatgt tgactcacca gatttaattc
     2701 tgtcaatttt tgagcatata agttaataca ttggattaaa tggtggctat gagccaaatc
     2761 aaaagcttct tttgctttga taaccaaaga ggctaggtaa tctatttgag atggtagggt
     2821 gacttcttga agatgatgat gtgtcgccaa tttgttgcca gtgtgaatga tcaagaagga
     2881 taaatctttg aatggccagg agaacgaatt aacctgattt ttttgtttat caatgtaaac
     2941 acaaccttgc tgtgactgag caatcacatc ataaccactg ggtcttaacc ccaaccccga
     3001 ccaggacgat tggtaataag cctctagcat atggtttaat cgaggggtaa tctcttgcag
     3061 tgaacaagtt gccaaatagc aggctaaaaa ttgcgcgctg gatgcgccca aaccgccttg
     3121 gccatgataa gggtcttgcc aggatagacc aaagggatgg cattgatttt tttgccacca
     3181 taatccagca ggtgattccg gatgaattcc ttctaatttt gcattttcag ttaatgtaag
     3241 agtaaagcaa gggcttgtcg ttaataggat agctggttct cccgcaatgg cagcgtattc
     3301 tcctaataaa aatgttttag cgggaatttg ccatttcata ctgcaacagg agcttgtcgg
     3361 atttcagcta ataggtcata agcgttattc aagctgactc gtttgttcag gttaagccat
     3421 tcttgcaatt tttctttgag cacgggcatt tcattttcat tagcccctgc aactaatagc
     3481 aaattatcaa tatgcaattt catatggcct tgaatgatcc cgtcagtgca taaagctttg
     3541 agtgcgccaa gattttgcac tagtccaact gctgcgatca cctgggagag ttgattggca
     3601 gaagtgatat ccatcattcg taaacacatt ttagccgtgg ggtgtaaaga ggttacgcct
     3661 ccaacagtac ctactatgat aggagcagtt aactctcctg tgagtatttc attttgatac
     3721 cgccagcgag taatcgcttt gtattgtccc gaacgtgccg cataggagtg tattccagct
     3781 tccacagctc gccaatcatt ccctgtagca attaaaaccg gatcaattcc attcataacc
     3841 cctttattat gggtagcagc gcgataggga tctatttctg caaagagtga ggcctcttgc
     3901 aatttttgac cgaggatggg gtcaatatta cgaatgatga cttgagcggt cgttaatttt
     3961 tgatcgttta aattggataa aatacacatg gtgacttctt caccagttat ttgttctatg
     4021 ggttgtttta aatattcaag tacttgattg atgatattgg cacccatagc atcgcagctg
     4081 ttcatcgtca aatgaattac tgccatatct tgcccatctt ctctctttaa atgacgtact
     4141 tgcaaatcgg taacaccgcc gcctcgtttg accatattgg cggcaacgtc tttattcgct
     4201 atttctataa aatattgacg atttttatta aagagatcag agaatctatg aaagtccttt
     4261 acttttgcca gttgaatttg cccaagaata cattctccgt gaacccaggt attgatttca
     4321 ccatgctgcc taatccattt cgctgatttg gaaagagcgg caataataga ggtttcttcc
     4381 actgccaaag gtattacata atcacgtcca tcaatattaa aattagtagc cacacccaat
     4441 ggaagctgaa aatatccaat cacattttca atcaatttgt cggccagatt taaatcttta
     4501 ataccgcctt gtttaagaaa tgttatgtct tcatgagtca aggcacctaa agcgcataac
     4561 ctttgaaagc gctcttcacg tgataacttt gagaatccgc gaaataattc accggcatta
     4621 gcagctatgg acatgccttc tcctttaaat catcaagaac tcggctgcca gtacaaaaca
     4681 tagcagtttt caattcatat tcaattgtat tcatttgggt cagtacttgc ccggttgagt
     4741 ccaaagccgc ttcaagcatg ggtttcgcga agccgactgt ggtagcaccg agcgcaaata
     4801 gctttgcagc atccaaacca tttcttacgc cgccagaacc ccaaatttcg aaagagggat
     4861 taagactaat ggcgttacga atgctttgta aagtatcaat accccaattt cgaaaagtat
     4921 cagcagttcg gtgtcttatc ggatccttgt ttgcccgatg tccttcaatt cgcccccaat
     4981 gcgtaccccc cacaccactg acgtcaacag cggcaacacc tatattattt aaccggagca
     5041 gcgtattttt tgaaaaacca caaccagttt cttttattat aactggagaa gcaatctttt
     5101 tgactaacgc ttccaatgct gtccagcacc cttgaaaatt agttgtccct tcaggttgaa
     5161 tacactcttg taatggattg caatgaacaa tcaatgcttc agcttgcaat gtatcaatta
     5221 agcgctggat tgcagaaatt ggagtgtcaa ttaactgagc gattcccaaa ttactgaaca
     5281 ggctgaccat ggggaaatca cgacggagtg gcgcccactc aaatgcggct tgtttatcgg
     5341 ttaattctct gcgttgtgag ccaacgccca ttgcccattt tgtttttgag caagcttcca
     5401 ttaatcgata gttaatttct aaagcattgg aatggcctgc tgtcattgaa ctgataataa
     5461 atggtttttc aaccgttttt ttaagccgga tgctttgtat ggaaatgtct ttaaaatcaa
     5521 ggtcaggtaa ggcttcatgc accaaagaaa aatgatcaaa gggatttaat tcgctgcttt
     5581 gattggctgg cattaaggcc agttctatgt gatcgcgctt acgctgttca aattggctgt
     5641 attcgttttg catgcctgaa tttacttttg aaaaatagcg tcaattttag cataaaatca
     5701 tgaaatagac cattgcattt ctattcgaca cagtgattta tttggtatca ttgatttaac
     5761 catgagttaa ctaatttttt cgtttagctg gcttcttggt aacaaattaa ggtaattatc
     5821 atgaaatcca aacttgaata ccatttctct gcaaacgcgg atggcgatga atcctgtatt
     5881 gaagatgttg atggaaatga atttactcca aatggttcac catcacatgg atggactgcg
     5941 tggactagat tttttggcca taaaaaccag tctcctaagt atgttgttaa acaacctaga
     6001 ccagataaag tccgttcctt gtcagatctt agagataaaa aacttaataa tgaatggaac
     6061 agaacacgct atcagcatga actggatata tggaatttgg tttatccaga taataaagcc
     6121 tatttgttta cagatagcgg aattcgattg gttctccctt atttacccgg taaaactcta
     6181 ttttctttta ctagtaggga agaagatcag ttaacttggt gtcaactaaa ccatgcagta
     6241 ttttgtgagt ttaagcgctt tactgaactt ggattgaagc actgcgattt aaaatatgac
     6301 aattttttga tagaaagaaa gcaagatggt acctttaaag cctacataat tgatctcgat
     6361 tcagtgggta aactaaagcc taatgatcag gattatgatt ggaatattgt cctggcatct
     6421 ctattcgacg ataccggact aaaaagtcca gatgtatgcc cacccggcta tattgctttg
     6481 aaatcaatcg atgttccagc agaaaatgag aatgaagcac gatcaaaact tgataaaata
     6541 aaaaaagaac tatcgcaaaa aaaacctacc ttgataagaa tcaagaattg ccagcaaaat
     6601 aagttggatt tttattttgt ttatggatgt ttgtatattg ggcaatgggg ctttactaaa
     6661 cttgatgaaa agatcattag ttcagttaat ctatctaatt tttctggttc tttagttgat
     6721 tgtagagccg cttctgttat tggtaatcat attaaattaa aacaaggcca cattagttac
     6781 agacttgacc tttatatcaa ctgtctgaat gagaaaatag ctgaactcac tctactcaaa
     6841 tctcaaaact cacctaaaaa gctgtatacc ccattttaaa tcagatcttg tttctatcat
     6901 gaagggatca aagcgtgctg ctgtgctgta agtataatat cttcttcttg gcaatgcctt
     6961 cttaggctat atcaccatcg ctatattcta tacataggcc agcagcgcct tatctcctac
     7021 cagcgataat agtaataagg tctgtagtag ggcctgtagt aaggtctgta atatggataa
     7081 ccccagcgat agtaatacgg tctatagtag tatccgcgat agtaatatgg cccccaatat
     7141 ctatagcgta cccagtaagc catttgagtc ggattggctt tattaattat cggattgata
     7201 tctctgttaa ctgctttggc atttgcaggc gttactaaaa tcaaagctcc aatcagaagg
     7261 gtaaaagcta gcatcagttt tattaaagag gtccctttaa agataaaacg tcccattttt
     7321 tactccttaa agcgtacttt atttaagtat agactacacg ggattatttg attaattttt
     7381 gtacataatc attatgggca tgagtttaaa ttttattgag gcagtaagtg gtttattagg
     7441 attaatgatt aaaaaaatgc ccgagccttt tggccgcagc tcgttcctca aggttctcta
     7501 tcttattgtg ttccaaggtt gttttgaata gtcgctgcga ttcgggatga aagttcatat
     7561 tgcgaataac gtgttctggt ttaagcaaag tatcaggtaa ctgataactg agttgatttt
     7621 ttttctgtaa ttgttgataa agagagttac ttaagcccaa gccatgttgt agcgcctggt
     7681 agaaacggga tatgggcccc tgtaaagcgg caatatggtc atagactatg cctggttgtg
     7741 gagctccacc aatgttttca agaaccagct ttaactcatc gagtttcgcc ataaataatt
     7801 tttgtttatt caaaacctta accaaggtat ctattggttt gaattgcttg gtctgttcaa
     7861 gtgatttcct tttgtcatga gattctttaa gctgtttttg agcaataaca cgtaataacg
     7921 aatctaccaa atgatgaagc atagccagaa tttgttttaa ttcgtctgtt cccaaagcaa
     7981 aagccaatcg ctccagattg aattcaaaag aatgcttggc ttctatccct aataattgaa
     8041 gcaggtattg ttgcaaatga cctaattggc ttaaggcatt ttgtgtatcc agtctgtatt
     8101 gcaaaaccag aataagggat ttgagtgagt ccaattcatc caagtcggat tcttccggta
     8161 gtcctttttc cagttgtaaa gactccagca gctgattaga ttgagtgagt tctgcttccg
     8221 cttgtttgat gaggttagat tgggtttcaa ttaacttttc attcatgaag ataacctgga
     8281 tataaaagag cactatctat attattatga taccatgaat cgtaagtttt tgcggtcttg
     8341 tggtattttg tattattcta ggaaaattca ataaaaagaa ataaatatgg aacagactag
     8401 cgcacagatt gttttcgaac aaaagaatgc aacataccag tgttgtggtt cctggtctgt
     8461 tttgaccctt gatggattac ctaaaaaatt tagtacaagc ccattacctg attcacaaca
     8521 aataactata aacggtgaaa aaatcagtgt ttttgatagc gccggggctt tggcattaaa
     8581 gaaatgcata gatgagctta aaaaaagaaa taaccaggtt gaattagtca atttttccga
     8641 tagccaacat caaatgctgg agctcattga gtccaaacaa gaaattcttt cctatcaatt
     8701 acctattgtt tccaaagaag gtgttcttta tcagattggt aaagagtcag aaaataaatt
     8761 gcatcaggtt gatggtttga ttattttgat aggagattta agcacaaggc ttttcgaagc
     8821 gtttgcatat tggcggcgat ttcatttgcc cagtattgtg tctgttattg attctgccgg
     8881 gctaaaagcg ttacctatca ttggattatt gtcttttttg ataggggtcg ttttagctta
     8941 tcaaatgggt ttgcaattag aaacttatgg tgccaatatt tttattgcct atttatctgg
     9001 catggcaatt tttcgggaat ttggaccttt aattacagca atcattgtag cggggcgcac
     9061 cagctcatca ttcacagctc aaattggcag catgaagatt aatgaagagg tagatgccat
     9121 cttgaccatg ggactttctc ccactgaatt attagtttta cccaaagtct tgggtttatt
     9181 aatagtgttt cctttattaa ttttttgggc agatgtcttt agcatttggg gggcaatgat
     9241 catgtccaaa aatatgttaa acattggttt cgaagatttt ttaactcgct tgagagattc
     9301 cgtaggactt aaccaaatga tgctgggttt gtataaagcc cctgccttct cgatattaat
     9361 tgctttagtg ggttgttttc aggggtttag agtagaatca agtgctgata gtataggaag
     9421 ccaaacgacc aaaagtgttg tgcaagcttt atttttaatt attattgctg acgctgcttt
     9481 ctccatagct tatagctgga tgggtttgta aatgagtgaa ccagttattg aaatcaaggg
     9541 aatgaaaaat tgtcttggcg gtcaatgggt gcacaccgat gttaatctca ccgtagaaag
     9601 gggagaaatc ctggctatta tagggggctc tggaagtggc aaaaccacca tattacgcag
     9661 tttgctgatg ttattgaaac cttctgcggg tagtattaaa gtattcggag aagaaattta
     9721 taatcttgat acggagcaag ctttcaggct taggcgtcga tggggcatgt tatttcagca
     9781 cagtgcttta ttttcagcta tgactgttct ggagaatgtg atgtttccaa tgagggaatt
     9841 cactacttta gatcaggagt ttatgcaaga actggcgctt ttaaaaataa ctttggtagg
     9901 cttatctaag gaagcagctg tgaaatatcc ttccgagtta agcgggggaa tgcaaagaag
     9961 agctgccgcg gcaagagcca ttgcaatgga tccagagcta ttatttctgg acgaaccaac
    10021 aacaggattg gatcctaggg gagccaggct ttttgatgat ttggttgttt ttttaagaga
    10081 tgcgttaaat ttaaccatag tcatggttag tcatgatata gagtctttaa aaaggacaac
    10141 agatcgtgtt gcatttgtag gtgatgggaa aattctcagt gttgagccca ttaatgaatt
    10201 gatgaaaaat aaaaacccgc taatcgccga atattttagc aaattctaag aaatactata
    10261 acagagggga caacttactt ggaatctaaa accaactaca ccatagtagg cctcatcgta
    10321 cttattctga cagctggcct attgtctgcg ggtttatggc tctctgtcgg gtttaaccag
    10381 aaagagtata cttcttatac tgtttatctc aaggagtctg tttctggtct cagtgtcgaa
    10441 tcgcctgtta aattcaatgg ggtgcaggtt ggttatgtca aggaaattaa attaaataaa
    10501 aatgatccca ggcaagtgga attattgtta aatatcgaaa agagtacgcc aataacaacc
    10561 agtacttccg caaccttaat ttcacagggt attacaggag tgacttatgt tgggttatct
    10621 gctggatctt cagagctgac tcctttacga aaaatgccgg gggagcccta tcctgtaatt
    10681 ccttccaaac catctctttt gaatcagttg gatgctgttt taaaggaggt tgctgagaat
    10741 gtaggtgcgg tgagtgaaaa ggctcaactt atctttaatg aagaaaacgc tgataatgta
    10801 agaaaatcat tggctaatat agagcgtatt acagaaataa tcgctgataa aggtcaaaca
    10861 attgatagtt ctttaaataa tcttgacgtg tttgtagcta atatggcgaa gacaagcaaa
    10921 caatttcctc agttgataaa agatttaaaa acaggtattt caaaattcaa atctttagct
    10981 gacaatatga gtgctgcggg taaggatgtt tcaaaaacga tgatagcggg caagaatact
    11041 atcgatcaaa tttcccaaca ggctatacct ccagcagtca ttttattgcg acgcttgaat
    11101 gccatttcag cgaaccttga aaaggtcagc aatgaaatga ggcaaaaccc ttctgtgatt
    11161 attcgaggta cgaaaacacc taaacttggg ccaggggaat aaaatatgag agttctcagt
    11221 agtgtttgtt ttatactagg aatattaaca ctgatgagtt gttcacctgt taaagtccca
    11281 gtgaccaacg aatatcaatt aacagcatac agtaccaaac aattgaccag gaaacccagg
    11341 cccatcacaa tacaagttac agttccggaa gcggtagcgg gatatcagac cgaagaaatg
    11401 ctttacatga agaaaccgtt taaattggag ccatttgtaa aaaacgcctg gacctcacct
    11461 cccgcggata tgctttttcc tttattagta caaagcctgc agagttccgg gtatttttat
    11521 gccgtcacat cgagccccta tagtgaagaa gcagattatc gtttggatac tcaactattg
    11581 aaattagagc aaaattttat caagaaacct agtgttcttg aattttctgc aaaaatagta
    11641 ctaacccata ttagtgataa tcaaattata ggctcacgaa ttgttagttt acaaatacct
    11701 tgttcacaag atactcctta tggcggagtt attgcggcga atcaggctac gtttcgattt
    11761 actgcaacgg caacagactt tgttgtatcg catattaagc gtgattagga atagccaaaa
    11821 aataaaaaga attgtacaat ggctctccat gattatcatg tgtagtttct aaaaaataat
    11881 aagaagagtt taaggagatt gtggaatgaa agccggatcg ttttataaac tgggactgct
    11941 tgtagcaagc gctgtattgg ttgcagcctg ttctaaaacc ccaggcagtg cggatggtgg
    12001 tgctgcggta ggtgatggtg atgctactgc ccaaggtttg gggcaaatga ctcactttgc
    12061 tggacaagag cctggtgagt cctatacaac gcaggcaccg cataatcaat tgtatttgtt
    12121 tgcgtatgat gacagcactt tggcatcaaa atatttacct tctgttaacg cgcaggcaga
    12181 atatttaaag actcacccag gtgctcgagt gatgatagct ggacatactg atgagcgtgg
    12241 aagtcgtgaa tataacgttg ctcttggcga acgccgtgct gatactgtag ctgaaattct
    12301 gcgtatggca ggtgttagca gacaacaaat tcgagtagtt agctacggca aagagcgccc
    12361 ggctaattat ggtcatgatg aagcgtcgca tgcgcagaat cgacgcgtag agtttattta
    12421 tgaggcaaca agatgattaa ttgcaaaaaa tccattatca ccctatgttt tgtactgatg
    12481 cttcctttcg ccttatgggc agaagcgcca gtaatagatg atagtgaaaa ttttgcaatg
    12541 attgacaggc aagaggagta tgatgctcct cttgtcaatc ccaagtatga tgatcctcaa
    12601 attgagaatg ctgagttgga tgggcctcaa aatgataact actcaaccga tacctctcaa
    12661 tcctatgatg aacccgcact tgtcaaagaa gaccgaagca atatcagtga caatgcaaag
    12721 ttaattgata aaattcagca gcttcaaaag gaaatacaag agttacgtgg ccagcttgaa
    12781 gtacaggcac acgatttgaa attattacag cagcagcaag tcgctttcta caaggatctg
    12841 gattctcgat tatccaattc ttcaacttca gcaaaaactg ttcaaaatga taaaccagca
    12901 acagatattt cattgggatc taattcacca gagactttaa aagtggcttc tcctcaaatc
    12961 aaagcaggat catctaatag taaacctcaa cctgttgttg ctgtttccag agctaatcct
    13021 gctgatgaac agataagcta tctggctgca tacgagttag ttaaaaataa acgctatgat
    13081 gaagcgatta agtctatgca gatttttgtg caaaaatatc ccagaggtgg ttacactgcg
    13141 aatgcagagt actggctagg tgaattatat ctcgttaaaa aagactattc taaagcaata
    13201 gaacattttg agattgtttt acagcaatat ccttcatcca gcaaggctgc agccagttta
    13261 ttaaaatctg gctatgctta tgccgagaaa ggtgacaagc aagaagctaa aaagcgtttt
    13321 caacaagttg ttaaaacata tcctgataca ccaacagcac aactggccag ctctaaattg
    13381 gaagcaatta atgctttatg aaacgattta atgaacaatt aagaattacg gagatttttc
    13441 attcattgca aggagaatca gtaacggttg gcttgcctac tgtatttgtt cgcctgacag
    13501 gctgtccatt gcgatgtcag tattgtgata cggcttatgc tttttcaggt ggtgaagttg
    13561 ttgaaataga tgatattttg aataaagtag cttcatatca atgtcagcat gtttgtgtta
    13621 ctggtgggga accacttgct caacctggct gtattccttt attaagcaaa ctttgtgatg
    13681 cagggtactc cgtttctttg gaaaccagtg gtgccagaga tatagcctct gtagatcaac
    13741 gagttatgat cgttatggac ttaaaaactc ctgattccag ggaagcagat aaaaacttgc
    13801 tgtccaattt gagttttctg aagcctactg atcagattaa gtttgtacta tgcagccgaa
    13861 atgattatga atgggcatgt agtatgctga gtgaatatca gttggctgaa cgggtacaac
    13921 tcttattttc tccgagttgg aatcagctaa atcctgctga tcttgcaaat tggataattc
    13981 aggataaatt accagttcgt tttcaactac aattacataa aattttatgg aatgatgcac
    14041 ctggtcattg acctcaagga gactaactat gcattggttt gctcaggcac catctaatat
    14101 cgctttgatc aaatatatgg gtaaaaagga tgaaaattcc aatttacctg ataattcttc
    14161 tctatcttac accttgagta atttattaag ttccgtgaaa ttagagaaat tgccgactaa
    14221 aaaagacatt tgggagccac ttagtattcc tggagcacca gaatttaacc tgtcagttga
    14281 agcgcaaaaa cggtttattg atcatttagt acgattgaaa gagtattttg gatatgtcgg
    14341 tgggttttta attcaatcca gtaataattt tcctcatagt agcggtttgg cgagttctgc
    14401 ctccagcttt gctgccttga caaagtgtgc atcaattgca ttaagtgatc taactcaaaa
    14461 acctttgcct tctattgatg agcaagcaca actgagtcga ttaggttcag gctcatcatg
    14521 ccgttctttt tatgcaccat gggcgttatg gaccggcgat aaagtatctg caatcgattt
    14581 gccttataaa gatctattac accaagtcat agtaattagc agtcaggaaa aagaaatacc
    14641 atcaagggta gcgcataagc tggttaaaac aagtcctttt tatgaaacgc gaagcgaaag
    14701 ggccgaggca aatttgaagt tgttactgaa cgcttttgaa aacaaagatt ggacgagcat
    14761 ataccaaatt tgctggcatg aatttctcga catgcatcaa ttatttaaaa cctgtgaaaa
    14821 accattttct tacattacag ataatacttt gcatatattg agcgttattg aaaaattttg
    14881 gaacgagaaa ggtgacggcc ccgtagttac tatggatgct ggccctaatg ttcatttgct
    14941 ttatagatca gatcaaacag atctcgcacg acaatttaaa tcggatcatt tggtaggaaa
    15001 ctatgatgtt ttatgatttt gaaaccacca ctttcggcaa gtggatttta gcaggtgaac
    15061 acgcggtagt aaggggacat gaagctttag tatttcctat taaagaaagg caattaactt
    15121 tgaaatataa cccttcctca ccgtccttga gcgcagaatt tcaaggcatt aatggttctg
    15181 atatgcattt attattttgg agcgtattag agcgtggcat gcatttgttg ggacgttcac
    15241 tgaatgaatt aggcggtcat ttttatttgg aaaccaatat tcctgttggg gttggcatgg
    15301 gtgcttcggc agcgctttgt gttgccatga gtcggtggta ctgtgcgcag caaatgatac
    15361 aagataatca atgtaatgaa tttgccaaac aattagaaca tctgtttcat ggtaaaagta
    15421 gtggcttgga tatagctggt gtagcatcag atactggtgt atattttaaa tccgggcttt
    15481 gtactccagt aaagcaaacc atttatccct attggtattt atcctcctgc aatcaaatcg
    15541 gcattacttc tcattgtata cagcaagttg aatatttatg gaataaaaat ccagaacttg
    15601 ctcaccaagt agataagcaa atggtagagg ctgttcagga agcaaaagct gttttggaag
    15661 agggggggag taatgcttta aaccgtttgg caaaagcgat aaataaagcc gcagattgtt
    15721 ttttacaatg gggtttggtt agtgagagct tgcaacaaca tatgaatcat ttgtttgctg
    15781 atggtgcaat tgctgttaag ccaaccggtt caggtggtgg agggtttgta ttaagccttt
    15841 gggataaaat tccctcatct catacagaat tgattccggt ctagctcctt tcaagcctaa
    15901 tcgacaaatt gataatatac ttcattttgc ttaatcatgc cgagctcata tcgcgcttgt
    15961 tcttcaagag cctgatcacc actttttaac tctttgatat ctgcttccaa agccttattt
    16021 ctggcagcta atttatcgtt ttgactttta tgagcctcca attttttttc gagtttaatc
    16081 cattggatga tatttccatc acctagccat agcttatgtt gtaaagcaac taaagcaaca
    16141 attaagataa tgaatatggg gcgcatagaa ttttctattg aagaatgtat ctaattacta
    16201 ttgtagtatc ttattaccac gaggggcaat atgtgttcag ggtatatagt attaaatttt
    16261 ttatcgaact gattttaaat tgtattttat ttatattacc ttggatttaa attagagctg
    16321 atgctgagtt ttattcagca ctcaacatca ccccaactta aacattaata atgctgaaag
    16381 cttgaactca tctctttaga atgttttttc cagcataggg taaagacgcc aattcattga
    16441 ttcttagtag ttgattatat ttcgcagtcc tgtctgttcg acacaaagat ccggttttta
    16501 tttggccaca accactggct actgcaagat cagcaatgaa ggtatcttct gtttctcctg
    16561 aacgatggga catgacacat cggtatccat tgtcgtatgc gagttttata gcttgcctgg
    16621 tttcgcttaa agtacctatt tgattgactt taattaatat ggcattagca attccctgag
    16681 caattccttc tcgcagaatt ttgggattag ttacaaataa atcatctccg acgagctgaa
    16741 ctttatttcc taaatgggtg gttaattgct tccatcctga ccagtctttt tcgtccagtc
    16801 catcttcaat actgactatg ggataactgg aaataagatt tgcatagtat tctatgagtt
    16861 gatgagaatc aaatttttga ttttcagaat acatatgata aaagccctca ttaaaaagct
    16921 cagaagctgc aacatccagg gcaaatacaa tgtcttcccc taatcgaaag cctgcttttt
    16981 caattgcttc gctaaggaga tccagcgctt ggcgattgga ttgaatatta ggtgcaaaac
    17041 caccttcatc accaacagcg gtatttaatc cttgcttttt taacacagat ttcagtacat
    17101 gaaaaatttc agttcccatt tgcaatgcaa ctggaaaatc aggagcgccg attggcatga
    17161 tcataaattc ttgaatatcc acattattat cagcatgagc gcctccattc agaatattca
    17221 tcataggaac aggcatggtc atcatgtcac cctgattaag ggtcatatac aatggctgat
    17281 taagagacaa tgctcttgct ctggcacatg ccagagaagt agccaggata gcattagctc
    17341 ccagatggga tttgttttct gtattatcca attggcataa aatgcgatcc aagttttcct
    17401 gatcctcaac cgacaatcct tgtaatgcct gatttatctc gttattaacg tgtttaactg
    17461 ctttttgtac acccttgcca gcgtatcgtt ttggatcatt gtctctaagt tcacaggctt
    17521 ccctgcttcc tgtggaagca cctgaaggaa cgctggctct tccaataatg ccagttgtga
    17581 gaataacgtc tgcctcaata gttggattac cgcgagaatc taatatttca cgtgcctgaa
    17641 ttttatgtat gtgcatgatc tttcctaatc tttttatatt tattaactta aatacctacc
    17701 accttaaggt ttacaagcgc attaactaat ggcattagtg gcaaaccaag aatagtatcc
    17761 tcgcttcctt gtacttcctt aaataaccac ttgcctaggc cttcatattg ataagcacca
    17821 caactttgat aaggtttttc cgcttgtaaa taggcttcaa ttgttttttc acttagatga
    17881 tgcagtgtca gtgtagccgt ttcatggtat tgccaaagta ttttgctctc tttaacaata
    17941 cacaggcaag caatttgttg atgttgcttc ccgcttaact ctctcaaatg gcttacagct
    18001 gtttgatgat tttgtggttt gttaaatact cttttaccag cgacgcataa ttgatctgct
    18061 gcaatgatat agtgctctgg ataatgttgg cttacatcca acgctttaat tttagccagg
    18121 gtaatcccta actcaactag ttcgtcggat ttatgccttg ttttaatttc ctcttcgtta
    18181 caattcgatg gtacaaccag gaaatccaag ccaagtgatt tcatgagttt atgtcttatg
    18241 gtcgaagagg aggcgagaat aatcggtttt tgttgtaaaa atttagacat agatggccac
    18301 cgatgccatg agtaaaaagc ctatgattac aaagctgaaa tcacttaaat tacagcaacg
    18361 ctctaccatg acacagcgtt ccagtccatg taaagttccc aaatagagaa atgtaccggc
    18421 agatgccgcg attaatatag ggtcgaataa agagtgagtt tctactccat gaccaaaata
    18481 ccaaccaaaa taaatgccta aaggtgtcat aaggctaaaa aagaaaaaaa ataccatact
    18541 ttttcgagtg ctcatcgagc ttttatttaa ttgaattgct atagccaaac tttccgccca
    18601 cttatgagtg atgatcgcta aaaatagcat aatgatcatg gaattgtatt gggtaaaacc
    18661 taaagcagct cccagcataa gcgagtggac agaaagcatg gcccaggcga gtattgcgaa
    18721 agcaggatgt tctgcatcgt gatggtggta tagttctttt cccaaatgtt caaaccacaa
    18781 gaaaataagg aatactactc cggtaataat aaacgcaaac ggatagttat aacccatttc
    18841 tttaaataag gtgtttgatt caggtaacat gtgaagaagt gctgcaccca gaaaaacacc
    18901 tgtagccaaa gtttcaccta tgggaaaatc aatgtgttta tcgtctttaa gtcgcttttt
    18961 aaatgggtac cagcctgcaa tgagaatgac tataaatatc gaaacagcaa aaatgatttt
    19021 taaactggtt gcggacaaca tgaagtcttc ctactttaaa atgcgtatat attatcctga
    19081 aatgactaat cgaatctaca gggcacagca gtattacatt gttaaaatct agcaattctt
    19141 gcatgccttc tgaatcttag tttgatggat gatgcatctc ttcatgcaag ccaaagagac
    19201 gcacttcctt tatatcaatt tgtgatttca gggttgaggt tactcgatta tgcaattttt
    19261 tccattgatt aaaatcattt gagcaaaaaa tatccaaact catttttcca tttaaataat
    19321 gtatattcca aaataaaatc tggggaaaat catggcgaat gtcattaaaa aatttttcct
    19381 ggagtattgc cctgtttggt aaatgcaagg atggacatga tatttcatcg tcttcagggt
    19441 caacatggac aatcacatct ttgacgcaat caatttcttt tactagagta tgatgcacat
    19501 gttgggcgat atagtgcccc tcggaaacgg atattttggg tgaaacaaga atatggacat
    19561 caataagcac atcactaccc ataaaccggc ttctcaattg gtgaatacgc tttactccat
    19621 caatgctttg tataactcgt tcaatttggg aaaataattc tgcatcaaca gctgtgtcta
    19681 ccaactcttt gacactgttc cagccatagc tccatcccat tttgataatc atcaggctta
    19741 cgatgacggc ggcaatggca tccaggtaaa caagccctgc caagctgcca attaatccaa
    19801 tcagcaccac tatggatgaa gcagcatctg atcgatgatg ccaggcattg gcggtgatta
    19861 attgggagct aatttttttt cctacatggc gggtgtagtg aaataatgct tcgtttgcaa
    19921 tgattgagaa acacactata ggaatcgata accagtttgg catggtatgg tctgatttaa
    19981 gcaattcatc aagtgcatcc caggcaatcc cacagcctgc caggattagt aaaagggaaa
    20041 gcaataaagt tgctgccgtt tcaattcttt gatggccata tggatgagag tcatcagcgc
    20101 taacgcttcc atattttgaa gcaaatacga ccatcacatc agtaattaaa tcagaaatgg
    20161 agtggactcc atccgcgatg agagcatgag aatgatataa aaaaccacca atcattttga
    20221 caacaccgag taaggcatta ataaaagcac cgataagggt cacttttttg gcttgccaat
    20281 acctgtcatg ggggtacatt gttgttcctt tttcaatcac aaaaataatt tggatttttg
    20341 taaataaaaa ttagaaggga aactatacca tgttatagcc tgagttccta tttatcttgg
    20401 attatcaatg attagtactc tgtcgaaaat aggaaaaaca agtgttcagg agcaagcatt
    20461 ccagtattaa attcaatata ta
//
LOCUS       DATNSN010000042        20480 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.042, whole genome shotgun sequence.
ACCESSION   DATNSN010000042 DATNSN010000000
VERSION     DATNSN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20480
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.042"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            88..213
                     /locus_tag="VAH76_001287"
                     /pseudo
     CDS             88..213
                     /locus_tag="VAH76_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
     gene            479..1234
                     /locus_tag="VAH76_001288"
     CDS             479..1234
                     /locus_tag="VAH76_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385924.1"
                     /translation="MYVFRRFSKHIITFLHSLELAFRFLGHLVDSTIKVIFGKLSIVW
                     PNTLEMIYYSGARLVILLSFIGILLGATVSQTVYALLKPFQLHQRVLPIVQNILTHEI
                     LPVLIGFILCIQAALHMVNTRLEHYQENPEAIILAQVLPIIVGMNIASLLLYVYLVSA
                     IFFSFYLTFHFMLGFTNNEFLSYVVSSTTLFDLIYSVVKTLILCIIVGLTSGYYYYEA
                     SIRHIYLRKAVSRILTRGSFWLIIASMYITLTF"
     gene            1302..1988
                     /locus_tag="VAH76_001289"
     CDS             1302..1988
                     /locus_tag="VAH76_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEO1385925.1"
                     /translation="MILGGTFFYIEYKRAQKQTFVMFFKGSLKGLVTTAPVTYRGVKI
                     GEVKVIEITENKEHSKVLIPVYVQFFVERTYGFSQDPIHLLIDNGYVANITKPNLLTG
                     VAEIELIKPTPAVKYKQTYYRSYPVFPTHNSAEKYTSMEEAFEAAKKAFEDVSELVRS
                     KEIQDTLEAIQKVSENLGQLASSLNQDLPSVVAYLNQSLKQITSAAYSTQNLTDYLSR
                     YPESLLRGKR"
     gene            1985..2710
                     /locus_tag="VAH76_001290"
     CDS             1985..2710
                     /locus_tag="VAH76_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEO1385926.1"
                     /translation="MKNNRHFLKFYCIKDLSWLSSVRTSFIYSPFQSIASSPFSSYIE
                     LRKKPVKFLLILLIALTLCDCGRSKETQFYLLTPISPQKNSRNSYNHLQIGVDEVSIP
                     DYMKKPQLMIHQTPHHLTLEEFNQWAGALDKNITLVLTTNLSTLMPGVVVQTSPWNNK
                     FHPDYHLQVMISQFEIDIHGNSILRAEYLIYRKEELIYKGNAYYHIKVPLISIETLVQ
                     SMNTNLTHLTEEIARFFIKNTTK"
     gene            2811..3221
                     /locus_tag="VAH76_001291"
     CDS             2811..3221
                     /locus_tag="VAH76_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEO1385927.1"
                     /translation="MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTAS
                     TVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQK
                     RRRSRRVKQKMLDEKSRQSQLKTLRKSPRSHDIE"
     gene            3488..4372
                     /locus_tag="VAH76_001292"
     CDS             3488..4372
                     /locus_tag="VAH76_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEO1385928.1"
                     /translation="MAGTSLLTLIDDIASALDDIAAMTKVATKKTAGVLGDDLALNAE
                     QIIGIHANRELPVIGAVAKGSAVNKLILIPLAILINFFAPALVGILLILGGLYLCFEG
                     FEKIVEKIFHKKNEPAKKKIDIQTIDMRQFEKEKISGAVRTDFVLSAEIIVITLGTVA
                     ATSLVNQIAVLTLISLIMTIGVYGLVACIVKLDDLGFILREKKHPLKQIGILLIGAAP
                     ILMKILSVAGTIAMFLVGGGIITHQITAIHHFTENLSAMMSIGIDFFIGIISGAITAA
                     LYFIFKKMKSKFKLMNTK"
     gene            4541..4996
                     /locus_tag="VAH76_001293"
     CDS             4541..4996
                     /locus_tag="VAH76_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO1385929.1"
                     /translation="MPDIKHYLKPMVLFSGLLVTPFAMAEATKAEETSTVKLSTILQQ
                     LSDQGYKNIHDAEIDDGVLTVEGSNEQGVKFEIHLDPATGKILKKQEHPQPKYSIIDI
                     TKKIENEGKFKIIEIEFDEGIYKIKGETDSHEKKELHIDPNTGETKTDH"
     gene            complement(5078..5851)
                     /locus_tag="VAH76_001294"
     CDS             complement(5078..5851)
                     /locus_tag="VAH76_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1385930.1"
                     /translation="MYKTVHFYSNNLKLEGKLEEPTGKCLGYVLFAHCFTCGKDIAAA
                     SRIASALVSNGFAVLRFDFTGLGSSEGSFSETNFSSNVEDLVAAADYLRTHYRAPVLL
                     IGHSLGGAAVLLAAKKISEVKAIATIGAPASAHHVKHHFSADLSKIESDGEAQVTLGP
                     RSFTIKKQFLQDIDRYQDTINSDAGKALLILHSPIDKVVSIKEAEKIYKAAKHPKSFI
                     SLDKADHLLSDKRDSQYAAEVIAAWASRYLTPSSEDQSG"
     gene            6277..6696
                     /locus_tag="VAH76_001295"
     CDS             6277..6696
                     /locus_tag="VAH76_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEO1385931.1"
                     /translation="MKTYRKELWFNIPERMGFINITDKVIECLKESSIQEGLILINAM
                     HITASVFINDDESGLHQDYKKWLEQIAPHEPIRQYRHNDTGEDNADAHIKRQIMGREV
                     VVAITEGRLDFGPWEQIFYGEFDGRRDKRVLVKIIGQ"
     gene            complement(6834..7571)
                     /locus_tag="VAH76_001296"
     CDS             complement(6834..7571)
                     /locus_tag="VAH76_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1385932.1"
                     /translation="MNNVNGQSMTRIDEIVPDIYRISTSISQEIIPGGFSFNQYLIID
                     KKPVLFHTGPLQLFPAISQAVATVIPVELLCFIAFSHFEADECGSLNEWLKVAPDAVP
                     MCGRIAAMLSINDLAIRSPKVMNDLEQVSLGKHNVQWLDAPHLPHGWDCGFLFETTTK
                     TLFCGDLFTQPGAEHQPITEEDILESSEMMRQAMDYYAHSNNQRQLFEKLASTHPKIL
                     ACMHGSAWKGNGFQLINALADRVCSNI"
     gene            complement(7664..8641)
                     /locus_tag="VAH76_001297"
     CDS             complement(7664..8641)
                     /locus_tag="VAH76_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385933.1"
                     /translation="MNLKAEVFLNQNCAEMMIKKAAQLILSSDLDFEYTRGIQDIQVD
                     LGPAFMFSPDEEKTLWVSGKNQETLEKDLATLNKSSVYFFRTGTQGGAGHWQVLYYEA
                     AKSGWVSYSSQSNHFQVTDSNGKLTASGKGLLVPHANWGKENGNYAFLLVNASAENII
                     HAANFVYILRTQNEVAAIEYCALNHEFHPEIKRTARAKAKPETRTKGEIHNPPKDLSC
                     ENLKTIYSKHVALLHVINRLETGSKSYNPYWMGSQAKLNTILGALGACYQKGLDVDSE
                     LSNSNSELSKAINIRRLPSLFSFWGGKIESPVESRKIIDENRTYRRNNP"
     gene            8701..8940
                     /locus_tag="VAH76_001298"
     CDS             8701..8940
                     /locus_tag="VAH76_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385934.1"
                     /translation="MIAWIIESPQYLSHLQDSRKEQSKEWEVLVLSSPVNAALCLIGK
                     EEKIDSVARSGSCPCINAPKSRVKIPKIEAPFKTA"
     gene            8953..9432
                     /locus_tag="VAH76_001299"
     CDS             8953..9432
                     /locus_tag="VAH76_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385935.1"
                     /translation="MKEKTAKQLKLFIIYLISYWLLSIISCLIAFGYDDSLRMLLASP
                     KSDLSGALLFFSSFIAAALLFVFRYKTFSDKPYPYFIFGFYVGNVSLLMLFILDAFIR
                     ELIVWKFPEFLLVFISPFVELVLSYLFFGFAFLAIIPAVTSAFILYGVQRKLLLPPI"
     gene            9608..9820
                     /locus_tag="VAH76_001300"
     CDS             9608..9820
                     /locus_tag="VAH76_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HEO1385936.1"
                     /translation="MGIVKISDELHEATKLMARAMSRSINSQAEYWIRIGKLAEENPS
                     ITYTDILKILVHQASLGEIYDVSEDV"
     gene            9801..10562
                     /gene="map"
                     /locus_tag="VAH76_001301"
     CDS             9801..10562
                     /gene="map"
                     /locus_tag="VAH76_001301"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEO1385937.1"
                     /translation="MLVKTSEEIKKMRVAGQLAASVLEMIEPYVIAGTSTRELEQICR
                     QYIVEDLKAIPSTLNHYGFPACVCTSINHVVCHGIPSEKKLKNGDIINIDVTVQKDGY
                     IGDTSKMFLVGNIKPFAKKLVEVTQECLYKAISIVRPGAYLGDIGNIIQTHAEQHGYS
                     IVREFGGHGIGKSMWEDPHIMHFGKPNTGLRLQAGMTFTIEPMLNLGRKEVKTLGDGW
                     TVVTKDHKLSAQWEHTILVTDNGHEILTLRADEHF"
     gene            10667..11119
                     /locus_tag="VAH76_001302"
     CDS             10667..11119
                     /locus_tag="VAH76_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO1385938.1"
                     /translation="MPCRKCDSATTYHLSGTYQLLTLPPRGHCYSKLLKHLTENNFDM
                     NKRDSGIIELLFTAERAQDIGHALERCYSQAELEDSKAVLLPIDIADRNVERVLNHTH
                     SLKKIMPSIFIKVFCLRDLCLTKKYPTISKQAHKNGLAIGCMTNAYKH"
     gene            11103..11980
                     /locus_tag="VAH76_001303"
                     /pseudo
     CDS             11103..11980
                     /locus_tag="VAH76_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(12052..13251)
                     /locus_tag="VAH76_001304"
     CDS             complement(12052..13251)
                     /locus_tag="VAH76_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1385939.1"
                     /translation="MKIMNLNLKKLIGYFLAIVIFFSVVLWFLSHQGISVERAKVKYD
                     HFIQYIEDDAKTRAIKIYSIVSPVSGELLRINLTEGDKVKKDDVIATIKSKIPALLDI
                     RAKQELEHELGTAEALYEQAKAESIRAEAALETAQSDFKRKEPLAKKGFVSETELEHA
                     QLEYQLRQKEHNAALRKLHAAEHYILKVKASLSASEGSEASQQDKIEIRSPVTGSILR
                     VIEKSSTTVEPGTKIMDIADVVGLEVVADILSDDAAKIPPYARVIIKRWGGPYPLEGR
                     VRIVEPSGYTKISALGVEEQRVNVIIDITTPYELWKNLGDGYRVDVEIILFESNHALV
                     VPMSALFRDGEDWAVFVVKGHRAYKKKLTIKYHNPDYAVIERGLELGDEVILYPSSLI
                     HDGVLIK"
     gene            complement(13251..15617)
                     /locus_tag="VAH76_001305"
     CDS             complement(13251..15617)
                     /locus_tag="VAH76_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEO1385940.1"
                     /translation="MVTALNKKLWRDVIKLRSQIITIALVVCSGVSVLIASVNTYVSL
                     RNAQQEFYSKYHFADVFASLKRAPDYLEKRITEISGVSRVETRIVKDVVLDLPWMPEP
                     AVGRFISISDKNKSGLNQLYLRQGRWPDSNRYDEILVNESFANSHDLKPGDNIIALLN
                     GHREQLKIVGIALSPEYVYAIRGEDLLPDNRHFGVFWMDRKALSTTFGMQGAFNDIII
                     KLAPNASEQFVMTQLERMFRSYGLTITYSRKDQVSDRFVTNEIKQQKIIATYIPPVFL
                     LVAAFLLNLVTGRLVSKEREQIATLKALGYGDLAIAYHYIKIILIIIVIGSLAGVSLG
                     AWFGNLMTELYAEYFRFPRFSYLFSNLAAFIGILVCFLAALLGTLRAIYQVINLMPAV
                     AMKPPAPIVYRATLIEKIKFFSRLPSSVKMVYRYIFRHFLRTMLTCVGIALAMAIIIL
                     GLFWQDAIRYLINTQFIMAQREHAMVSFNNPVNKIAVTELNKITGVTSSEGYRIVPAR
                     LTYQHRSELSSLFGIAENAELKILLDKHLNRMIIPEQGLLISEGLAERLRLAVGDRLD
                     IELLEGNKAKTQLKVQGVINDYVGMFAYIEIKALNRLLNEDRLVNAAAITVDTKHVKH
                     LYKKVKEIPKIGTITFKTSIIRTFEETFAKHILVFTTILASFAIIIAVSVVYNNASIT
                     LAERARELTTLQVLGFTQGEVSTLLFLNHIFEIIISTPLGLVIGYLMSWSILQLMQTD
                     WFKIPFVIELKTYVISIMVIFFSSLISFYIIQRKASHLNLTAVLKVAD"
     gene            complement(15611..16321)
                     /locus_tag="VAH76_001306"
     CDS             complement(15611..16321)
                     /locus_tag="VAH76_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1385941.1"
                     /translation="MSPIPAFVIRNLTKTYLMGEVVIHALRCINLDLYQGELVVFLGP
                     SGSGKSTLLNIIGGLDVPSSGEVIYKDHLITSASQHDLTRYRREHIGFVFQFYNLISN
                     LTALENVSIVTEIAENPMHPEDAIKLLGLEHRMHHFPAQLSGGEQQRVAIARAIAKNP
                     DVLLCDEPTGALDCETGKIVLATLALANQKLKTTVVIITHNIVISEMADRVIHIADGV
                     ISNISKNKFKRKPEELEW"
     gene            complement(16795..17541)
                     /locus_tag="VAH76_001307"
     CDS             complement(16795..17541)
                     /locus_tag="VAH76_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385942.1"
                     /translation="MKRYIWFHPSYVLESAEATLLGQQTNAKDHGYILLNDQELGQVT
                     CEDHLTIMGHSSLESTPSEDTGLYMQGDTADGLIRRLTANGLKEAPKILSLESCRAGI
                     SGGLAQQLSCSLFFKYTLIEANLNSVGRKPMDTQWNFPTDSLGRAVLKLDNSPWMFYL
                     GGFLVGRLTHDTYTMENLFSLLSPPTFHKHFFANYQPGFFGGRSGRHCLFNKTTITLE
                     KASNFAHEDQDSASAKALEVVLKKLYSQPG"
     gene            complement(17802..19238)
                     /locus_tag="VAH76_001308"
     CDS             complement(17802..19238)
                     /locus_tag="VAH76_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385943.1"
                     /translation="MTPLFPQKGQPIAIKQGRSGSCFLLACLDCLLNGEAEGAGVVRK
                     KFKQTTSGVTLRIKRTDNTMKVQWEKMAGKYTYSYDPRTDEDVIFIDNEKLKEIDNSK
                     KGSSSNSLAVKILEHISAYYYQIAWDGESLLAHKLHPKFRNNPIAKQSDTAFLAKLLG
                     VHVTSFYDPHKLVKLKTIDPMIPVYVGMNFGKLDRLGRHRRHALRVGNIKPLDDGEYE
                     VTLINPWNNQKQTTCLLTDLRKRKCHFAVFDSSPQKKLLTEIFLEYKTEIGCELFRNK
                     ELAFLLCEMKQCGLLDSELAKYPLSSCMVLYKKLPNLVANYRCLPYSERYNMLMCVAS
                     TNGHVRLFNKLMQGSSVVDKPSVARSPQRPYLSAKSFFADLFASLEASLERVHYNDLL
                     KTLRVNYGHLRSVNTALNQVENRQGIYSKMELKNMNQRLDRLGFFMKCHTDPRGHRNS
                     DLGQQPNFFDQSTQKVNVKNHQNKGVSL"
     gene            complement(19753..20106)
                     /locus_tag="VAH76_001309"
     CDS             complement(19753..20106)
                     /locus_tag="VAH76_001309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385944.1"
                     /translation="MIKIYNNCTLKNQLSHGLKGQFTVIKGQCELIQARVDTGKPPIR
                     LVVWCFYGRASRIVVVYGKQFQSFFAPLMIILISEICSNPPWSPVFKKHTLSMPSIVM
                     NKLVVSGLIATFGGQ"
     gene            20174..20398
                     /locus_tag="VAH76_001310"
     CDS             20174..20398
                     /locus_tag="VAH76_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385945.1"
                     /translation="MKPIKNSKEQEYIAPEAQDPRLSYTEDNRRPLTSTTSAKHPSSL
                     FGKSEERSARESYDQYGNNVSTHWTQGPWN"
BASE COUNT         6373 a         4153 c         3762 g         6192 t
ORIGIN      
        1 ttcagaaaat aaataattta ggttattcac catgaaagca aaatacgccc ccacagagcc
       61 tggactccaa aagttacctc ctgaaatcaa ggccatgcac atcgccgtgc ttttcaattc
      121 ttttcccaac ccagtcaaag tgctgtacaa caaaactcgg aagaagaaaa tttaagtgct
      181 gatcccaagg cctggccatg tgagccattt tagttcgggt cttggcccga tatttaccga
      241 atgcttctac ttaattcttc gatatttatt atatttacaa ccttattttg atcctccact
      301 cacccgaata tcactagcaa cgaactaaac agagtgttat tcttggttgt agtaaaagag
      361 caatacgtct cactgctatt aagttaagaa ttaacttctt tttgcctgat acaagcatct
      421 tgctaacgaa gctattattc ttgtcacggc attttgaaag aggactaatg gattagccat
      481 gtatgttttt cgccgttttt cgaagcatat tattactttt ctccattcgc ttgagctcgc
      541 ttttcgattt ttaggccatt tggttgatag cactatcaaa gttatttttg gcaaactgtc
      601 catcgtctgg cctaacacac tagaaatgat ttactattct ggagcgcggc ttgttattct
      661 actgagtttt attggtatat tattaggcgc tactgtttca caaacggttt atgcgctctt
      721 aaaacctttt cagctacatc aacgagtact tcccattgtt caaaatattt taacccatga
      781 aatattaccg gtacttattg gcttcatttt atgtatccaa gccgccttgc atatggttaa
      841 cacccgtttg gagcattacc aggagaatcc agaggcaatt atccttgctc aagtattacc
      901 aatcattgta ggtatgaaca ttgcctcctt actgttatac gtttatcttg tatccgctat
      961 tttttttagt ttttatctca cttttcattt tatgctaggg tttacaaaca atgaattttt
     1021 atcctatgtt gtcagctcga caaccttatt tgatctgata tactcggtcg ttaagacatt
     1081 gattttatgc atcatagttg ggttgacttc tggttattat tattatgaag catctattag
     1141 acatatttat ttgagaaaag cagtttctcg aattttaacg cgtggctcat tctggctgat
     1201 cattgccagt atgtatatta ctcttacgtt ttagggacga cgtgcataac gaacgattct
     1261 atacattagt gggtattttt atagtaggcg ccctatgttt aatgattcta ggcggtactt
     1321 ttttttatat agaatacaaa cgcgcacaaa aacaaacttt tgtcatgttt ttcaaaggct
     1381 ctttaaaagg gctagtcact actgccccag taacctatcg aggcgtgaag atcggtgaag
     1441 tcaaagtcat agaaatcact gaaaataaag agcacagtaa agtactgatt cctgtttacg
     1501 tccagttttt tgtcgagaga acctatggtt ttagccaaga ccctattcac ctgcttattg
     1561 ataatgggta tgtagcaaac atcaccaaac caaacctgtt aacgggcgtt gctgaaattg
     1621 agttgattaa gccaacccca gcggtaaaat ataaacaaac atactaccgt tcttatcctg
     1681 tttttccgac tcacaattcg gctgaaaaat atacatctat ggaagaagcg tttgaggccg
     1741 cgaaaaaggc ttttgaagac gtcagtgaat tagttcgttc caaagaaatt caagatactt
     1801 tagaggccat acaaaaagtt tctgaaaatt tagggcaact agcctctagc ttaaaccaag
     1861 atttgcccag tgttgttgcg tatttaaatc aaagcttaaa acaaattacc agtgccgcct
     1921 actccactca aaacttgaca gattatttgt ctcgatatcc tgagtcttta cttcgaggca
     1981 aaagatgaaa aacaataggc attttttaaa attctattgc atcaaagatc tgagttggct
     2041 aagtagtgtt cgaacctctt ttatatattc cccttttcag agcatcgcat cctcgccttt
     2101 ttctagctat attgagcttc gaaagaaacc tgtgaagttc ctgttaatct tgctcattgc
     2161 cttaacactc tgcgattgtg gtcgaagcaa agaaactcaa ttttatttat taacacctat
     2221 ttcccctcaa aaaaacagcc gtaactccta taatcattta cagattggtg ttgatgaagt
     2281 gagtattcct gattatatga aaaaacctca attaatgatt caccagacac ctcatcacct
     2341 aactctcgaa gagtttaacc aatgggctgg ggccttggat aagaatatta ctcttgtatt
     2401 aactactaat ctttcaactt taatgccagg agtagtagtt cagacctcac cctggaacaa
     2461 taaattccac ccggattatc atcttcaagt aatgatttca caatttgaaa ttgatattca
     2521 tggtaacagt attttaagag ccgaatacct tatttaccga aaagaagagt taatctacaa
     2581 aggaaatgct tattaccata ttaaagtccc tttaatcagt attgaaacgc ttgtgcagag
     2641 catgaatacg aaccttactc atttaactga agaaattgca cggtttttta taaaaaacac
     2701 gactaagtaa tatactcata gtatcttcaa atcaagaaaa attattttga ttgaattcag
     2761 ctattataat gctttaaagg cattctttta tgggataaca aagagacttc atgattggca
     2821 aagataaatg ggaaatactg accgagaaga tgaccaatct tcatatctat gaaacagatc
     2881 tcagcgaaaa atttatacta ggcagtggta aaggcggaca aaaactacat aaaacggcat
     2941 caacagtcta tttgaaacat ttgcctactg gtatggaagt aaaatgccag gaatccagga
     3001 gtcgtgaaga taaccgctat tttgcgaggc aacggctttg tgagaaatta caagccgtat
     3061 tcagcgatga aaaaacaaag acacaacaaa gaattgaaaa gataaaacgc cagaagagac
     3121 gacgctcgag aagagtcaaa caaaaaatgt tggatgaaaa atcaagacag agtcaactca
     3181 agacattaag aaaaagcccc cgatctcatg acattgaata ataggcacat taaccactta
     3241 tcattttagt taaatcaaaa taccgggtaa catctctaaa gttttttgta gactcctgaa
     3301 ctttaaccca gggacattat tttcagttcg cttcgatcaa ttccttctcc cacgtctggt
     3361 gattttgaaa ttgaaatttt tatatcgtca ggttcgagtc tttgaaaaga agataagaca
     3421 tctttttgca atgctttgaa tttgctttag tattgaaagg aatcattaaa ttaaggtcag
     3481 ctccgatatg gcaggaacaa gtttattaac attgattgac gatattgcat cagcactgga
     3541 tgacatagct gccatgacga aagtggcaac taaaaaaaca gccggtgtgt tgggcgatga
     3601 cttggcactc aacgccgagc aaataatagg catacatgcg aatagagaat tacctgtcat
     3661 aggagctgta gccaaaggct ctgctgttaa taaattaata ttaattccat tagcgatttt
     3721 aataaatttt ttcgcccccg cactggtggg tatcctattg attttgggcg gattgtattt
     3781 gtgttttgaa ggttttgaaa aaattgtaga aaaaattttc cataaaaaaa atgagcctgc
     3841 aaaaaagaaa atagatatac agaccatcga tatgcgccaa tttgaaaaag aaaaaattag
     3901 cggtgctgta cggacggatt ttgtattatc agctgaaatt attgtgatca ccttaggcac
     3961 tgtggctgcg acatctctgg ttaaccaaat agcggtatta actttgattt cattaattat
     4021 gaccattggc gtttatggct tggttgcgtg cattgtgaaa ttggatgatc tgggcttcat
     4081 tttacgggaa aaaaagcacc cacttaaaca aattggaatt cttttgattg gagcagcccc
     4141 aatacttatg aaaattttaa gcgtggcagg aacaattgcc atgtttttag taggaggggg
     4201 gattattact catcaaataa ctgccattca tcatttcaca gaaaacctgt ctgcaatgat
     4261 gtctatagga attgatttct ttataggaat tatttcaggt gcaattacgg ctgctctata
     4321 ttttatattc aagaagatga aatcaaaatt taaactaatg aatacgaagt aagtactgta
     4381 agagtcgaaa tccattgtaa aatcaaagta attttgcatt ccatcgctca aatgactata
     4441 gttaaacaat gattttttaa caccaaacct catcaagcga ttcttttttt gcgatttatt
     4501 ttggatttta ataccacact atcaatagga gatatgcagg atgccagata tcaaacatta
     4561 ccttaaaccc atggttttat tttcaggact attggttact ccttttgcca tggccgaagc
     4621 aacaaaagct gaggaaactt ctactgtaaa attaagtact attcttcaac agttgtctga
     4681 tcaggggtat aaaaacattc atgacgctga aattgacgat ggcgtattga ctgtcgaagg
     4741 cagcaatgag caaggcgtta agtttgaaat tcaccttgat ccggccacag gcaagatttt
     4801 aaaaaagcaa gaacatccac aaccaaaata ttcaatcata gacattacca aaaaaattga
     4861 aaacgaaggt aaattcaaaa tcattgaaat tgagtttgat gaaggtattt acaaaataaa
     4921 aggtgaaaca gacagccacg aaaaaaagga actgcatatt gaccccaata ccggggaaac
     4981 caaaacagat cattaactcc atatgaaaga gcaagtcttt cctttccctt agcacaacca
     5041 attatttccc taaggaaaag ggagaggctt gccccactca accactttgg tcttctgatg
     5101 aaggagttaa ataacggctg gcccaggcag caataacctc tgcagcatat tgggagtctc
     5161 ttttgtcact taacaaatga tcagccttat caagactgat aaagcttttt ggatgcttgg
     5221 ctgctttata gattttttcg gcctctttaa ttgataccac tttatctatg ggggagtgca
     5281 aaatgagtaa cgccttacca gcatcgctat taatcgtgtc ctgataccgg tcaatatctt
     5341 gcagaaattg ttttttgata gtaaacgagc gtggaccaag cgtaacttga gcttcaccat
     5401 cagactctat tttacttaaa tcagcagaaa aatggtgctt aacgtggtga gcactggcag
     5461 gcgcaccaat agtggcgatt gcctttactt cagaaatttt cttggctgca agcagtacag
     5521 cggcacctcc aagactatgg cctatgagca gaacaggagc tcgataatgg gttcttaaat
     5581 agtcagcagc agcaactaaa tcttcaacat ttgaagaaaa attcgtttcc gaaaaggaac
     5641 cctcgctgct gcccaagccc gtaaaatcaa agcgtaagac cgcaaatcca tttgagacca
     5701 aagcgctggc aattctgctt gcagcagcta tatctttacc gcaggtaaag caatgagcaa
     5761 ataaaacata accaagacat ttccctgtcg gttcttccag tttcccttcc agcttcaaat
     5821 tgttactgta aaaatgaacg gttttgtaca ttgcatattc cttatttatc ttgattaggt
     5881 gctcatacag aacattcaaa tctgtttata aatccttttt cttgctctga aattaatttg
     5941 attgattact gatccagagt ttggatagtt tcaaagtgtt cacatactaa caaatatagt
     6001 catatttaac ccaatttccc aatctatttc gactctttat tcactttgtg tcatggtgtc
     6061 tcgaagtgaa atcaaagcaa cgatttatct gcactacacc acacttaaag cgccgctcgt
     6121 ttaaaatcat gggagattga ccaccctctt tttcccatca ttatgattcg cgccttaaga
     6181 cccggaggtt tccagaaatt ttcatctatt tgaaataaaa ccaagaatac tatatgtttg
     6241 ccagatattg atttattgcc aaatcattgt cacatcatga aaacgtaccg aaaagaacta
     6301 tggtttaata tcccggagcg tatgggattt attaatatca cagataaagt cattgaatgc
     6361 ttaaaggaaa gtagtattca ggaaggttta atattgatca atgccatgca tatcaccgct
     6421 tccgtattca tcaatgatga tgaaagcggc ttgcatcaag attataaaaa atggcttgaa
     6481 caaatagcac cccacgaacc cattaggcaa taccgccata atgacaccgg cgaagataat
     6541 gctgatgccc atatcaaacg acaaatcatg gggcgagaag tggtcgtcgc tattacagaa
     6601 ggaagactgg attttggccc atgggagcaa atattctatg gtgaatttga cggtcgccgt
     6661 gataaaagag tccttgtaaa aattatcggc caataaatca ggactcgaag gcatcctgag
     6721 taattgaagg ataaaaaatc gggattttcc ttagcaagcc atgccctgat tctcaccata
     6781 gaaaacagaa accccaacca tgatctgcta cttaacggga gtcgcctgct ccattagatg
     6841 ttactgcaga ctctatcagc caaggcatta atcaactgaa acccatttcc tttccaggcg
     6901 ctcccatgca tacaggccaa tattttgggg tgtgtgcttg ctaatttttc aaataactgg
     6961 cgctgattgt tactgtgcgc gtagtaatcc attgcttgtc gcatcatttc actgctttcc
     7021 aagatatcct cttcagtgat aggctgatgc tctgctcctg gctgggtaaa cagatctcca
     7081 caaaataggg ttttggttgt ggtttcaaac aaaaaaccac aatcccaccc gtgcggtaaa
     7141 tgcggcgcat ctaaccattg aacgttgtgt ttacccaaag aaacttgctc cagatcgttc
     7201 atgaccttag ggctacgaat cgccagatca ttaattgaga gcatcgcagc gattcgccca
     7261 cacataggaa ctgcatcagg agctactttc aaccattcgt ttaatgaacc gcactcatcc
     7321 gcttcaaaat gtgaaaaggc gataaagcat aacaattcta cggggattac tgttgcaacg
     7381 gcctgactta tcgcaggaaa aagctgcaaa ggacctgtat ggaataacac aggcttttta
     7441 tcaatgatta aatattgatt aaatgaaaaa ccaccaggaa tgatctcttg tgatattgaa
     7501 gtactgattc gatatatgtc tggcacaatt tcatcaattc ttgtcattga ttgtccattt
     7561 acgttattca tccattcacc tttatgatta aatgacagtt cactattatg ttttttgcaa
     7621 caccaacaac ataaaagacc atatcctgcc ctatgagcag tttttatgga ttatttcgtc
     7681 ggtaagtcct attttcatca attatctttc ttgattctac cggggattca attttccccc
     7741 cccaaaaact aaaaagcgat ggtaagcgac gtatattgat tgcttttgat aattcgctgt
     7801 tgctattgct taattcacta tcgacatcaa ggcctttttg ataacaagct ccaagcgccc
     7861 ccaaaattgt attgagcttc gcctgactgc ccatccagta aggattgtaa ctcttacttc
     7921 ctgtttccag acggttgatg acatgcaaca aggctacatg cttactgtag atagttttaa
     7981 gattctcgca agacagatct ttggggggat tatgaatctc accttttgtt ctggtttctg
     8041 gttttgcttt ggctctggca gttcttttta tctctggatg aaactcatga ttaagtgcgc
     8101 aatattcgat tgcagcaacc tcattctgag ttctcaaaat atatacgaaa ttcgctgcat
     8161 gaatgatgtt ttctgcactc gcattaacaa gtaaaaatgc atagtttcca ttttcttttc
     8221 cccaattggc atggggaaca agtagtccct taccactggc tgttaacttc ccattgctgt
     8281 cagtcacttg aaagtgattg gattgagatg aataactaac ccaacctgat ttcgcagcct
     8341 catagtacag gacttgccaa tgaccagccc ctccttgagt accagtccga aaaaaataga
     8401 cactgctttt attaagagtc gccagatcct tttccaacgt ttcctgattt ttcccactta
     8461 cccatagtgt tttttcttca tctggactga acataaaagc aggacccagg tcaacttgaa
     8521 tatcttggat acctcgagtg tattcaaaat caagatcact gcttaaaatc aactgagcag
     8581 ctttctttat catcatttct gcacaatttt ggtttaaaaa tacttcagct ttcaaattca
     8641 ttggaaaatc ccagccattt ttggattatt ataatacaaa ataatcgcat aaaaagctaa
     8701 gtgattgctt ggattataga atcaccccaa tatttgagtc acctgcaaga cagcagaaag
     8761 gaacaaagca aggagtggga agtcttagtc ctttcctcgc cagtcaatgc cgccttatgc
     8821 ttgataggca aagaagaaaa aattgactct gtggcccgtt cagggtcatg cccctgcatc
     8881 aatgccccca aaagtcgtgt taaaattcca aagatagaag ctccatttaa aacagcttga
     8941 ttggaattgg ttatgaaaga aaagactgcg aaacaattga aattgtttat tatctatcta
     9001 attagctatt ggctattaag cattatttca tgccttatcg ctttcggtta tgatgattcc
     9061 ctacgcatgt tgcttgcctc gccgaaatca gatctcagcg gtgctttatt atttttctct
     9121 tcatttattg ccgccgcttt gttgtttgtt tttcgataca aaactttttc agataaacct
     9181 tatccttatt ttatttttgg gttctacgta ggaaacgtct cgttactgat gttattcata
     9241 ttggatgctt ttattcgcga actcattgta tggaaattcc ctgaattctt acttgttttt
     9301 atatcaccat tcgttgaatt agtactctct tatctatttt ttggctttgc attcctggca
     9361 atcattccgg cagttaccag tgcttttatt ctctatggag ttcagagaaa attattattg
     9421 ccaccaatat aaacctccag agtcttctct ttttcatttg tcatcgctcc cttattctcg
     9481 ccttggaaaa aaggaagcct gcccctaatc tattgcccga tttatttatt ccccacaaaa
     9541 atatatttta taatgaaatg tatcatttta tattaaaacg ctatattata cgttttttgg
     9601 agaaaatatg ggcatagtaa aaatttcgga cgaactgcat gaagcgacca aattgatggc
     9661 aagagccatg agtcggtcaa tcaactcaca agcagaatat tggattagaa taggaaaatt
     9721 ggcggaagag aatccatcca ttacctatac tgacattctt aaaattttag tccatcaagc
     9781 ttcactaggt gaaatatacg atgttagtga agacgtctga agaaataaaa aaaatgcgcg
     9841 ttgcggggca gttggcagca tccgtacttg aaatgataga gccttatgtc attgcaggta
     9901 ccagtaccag ggagcttgaa caaatttgtc gtcagtatat tgtcgaggat ttgaaagcga
     9961 ttccctcgac gttaaaccac tatgggtttc ccgcatgcgt ttgcacttcg ataaatcacg
    10021 tggtatgcca tggcatcccc tcagagaaaa aacttaaaaa tggcgacatc attaatattg
    10081 atgtgactgt acaaaaggac ggttatattg gggataccag caaaatgttt ctcgttggca
    10141 acataaagcc atttgctaaa aagttagtag aagttaccca ggagtgccta tacaaagcta
    10201 tttcaatagt tcgcccaggg gcttatctcg gagacatcgg aaacataatc caaacacacg
    10261 cagaacaaca tggatattca atagtacgag agtttggtgg gcatggaatt ggtaaatcca
    10321 tgtgggaaga tcctcacatt atgcacttcg gtaaaccaaa taccggatta cgattacaag
    10381 caggaatgac tttcactatt gagccaatgc tcaatttggg tcgtaaagag gtcaagactt
    10441 taggggatgg ttggactgtt gtcacaaaag accataaatt atccgcacaa tgggagcaca
    10501 ccattttagt gaccgataat ggacatgaga tacttacatt aagagcggat gagcactttt
    10561 agacatctgc cctcttgttc actaattact gttcccccaa gcctctcggc ggaaaacata
    10621 tactataatc aaactaaagg aaatctagtc aatcaaggga aatagcatgc cttgtcgaaa
    10681 atgcgattca gcgacaacct accatttatc cggaacctac cagttactca ctttaccccc
    10741 acgaggtcat tgctattcaa aattattaaa gcatttaacc gaaaataatt ttgatatgaa
    10801 taaaagagac agcggaatta tcgaattatt atttactgct gagagagcac aagacatagg
    10861 tcatgcactg gagcgatgtt attcacaagc agaacttgag gacagtaaag cagttttgtt
    10921 gccaattgat atcgcggaca gaaacgtaga gagagtactc aatcatacgc attcattaaa
    10981 aaaaattatg ccatcgattt ttatcaaggt tttttgtttg agagacctat gccttacgaa
    11041 aaaatacccg actatatcaa agcaagcgca taaaaatgga ttggctattg gatgcatgac
    11101 aaatgcttac aaacattgaa agagcaaaac ttcaattaat tgatgaaacc agcgatatgg
    11161 tcattataac caaagcggat ccacttgatg cacctaatgg tccggaagtg gtttgggtaa
    11221 ataaagcctt tgaaaaaatt tcaaaatata aagccaatga aattattgcc aaacaccacg
    11281 tatccttcaa ggtaaagaaa ccaatcaaaa cacattaaaa atgataagag aagcaatgaa
    11341 agctcaaacg agtatcaatg tggagttatt aaattatact aaggatggga caccttattg
    11401 gattaatttt tcaatagtat atttgagaga cgggcagggt aatttatgtt atttgggtgc
    11461 aatagaaaga gatatcaccg caatcaagaa tttaaatttt caattatctg aaaaagcaaa
    11521 gactgatccg cttaccaacg tttacaatca ggaaaatttc ttcatcgcag ggaatgaagc
    11581 tattaaaaat tttcatcaaa accaggaagc agttggatta ttatttttcg atgtggatga
    11641 ttttaaagga ataaacgata cgcaagggca tatctatggc gataagatac taaaaaaagt
    11701 tgcccaggaa tctttaaagc ttgtccgtaa aacggataac gtatttcgat atggcggcga
    11761 tgagtttgct attatttttt caggcattga tgtgaaaatc ctggaaagaa aagcacaaca
    11821 actacaaaaa aagttagcaa aatctcacat ctcagtgagc attggaggga ctatttcaaa
    11881 gcaaagtgac aaatcgataa ataccctctt ggcgagagcg gatgaagcac tctattacgt
    11941 caaagccaac aaaaaagggt tgttttttat cagagaataa tcacattttg acgctgctaa
    12001 attacgagga aattaaaccc ggagacattc aattctttga aaatgacctc attatttaat
    12061 taagacccca tcatgaatta aagagctggg ataaagaatg acttcatcgc ctaactctaa
    12121 accacgctct attaccgcgt aatctgggtt atgatatttt atagttaact tcttcttata
    12181 agctctgtgc cctttcacga caaaaacagc ccaatcttcc ccgtctcgaa ataaagcact
    12241 cataggcact acaagagcat ggttcgattc aaaaagaata atttcaacat ccacccgata
    12301 tccatcacca agatttttcc ataattcata tggggtcgtg atatcaatga tgacattgac
    12361 tcgttgttcc tcaactccaa gcgctgatat tttagtataa ccgcttggct caacaattcg
    12421 aacgcgccct tccaaaggat aaggccctcc ccatcgtttt atgatgactc ttgcatacgg
    12481 aggaattttt gcggcatcat cgcttaatat gtcagccaca acttcaagac cgaccacatc
    12541 ggcaatatcc ataatttttg tgccaggctc aacggtagtt gaactttttt caatcactct
    12601 caaaatcgag cccgttacag gtgaacgaat ctcaatcttg tcttgctgac tcgcttcact
    12661 gccctctgat gccgaaaggg atgctttaac ttttaaaatg taatgctctg cagcatgtaa
    12721 cttacgcaat gcggcattat gttctttctg tcttaattga tattccaatt gagcatgctc
    12781 caattctgtt tccgaaacga accctttttt tgccagtggc tcttttcttt tgaaatctga
    12841 ctgggccgtc tccagtgctg cttcagcgcg aattgactct gcctttgcct gttcatacaa
    12901 ggcctcagca gtgcccaact catgctctaa ttcttgcttt gctcgaatgt ccagcaaagc
    12961 aggtatttta gacttaatag tggcaattac gtcatctttt tttactttat caccttccgt
    13021 caaattaatg cgtaacaatt ctcctgacac tggagacaca atactgtata tttttatagc
    13081 acgcgttttc gcatcgtctt caatatactg aatgaagtga tcatatttca cttttgccct
    13141 ttcaactgaa ataccttgat gagaaaggaa ccagagaaca acactgaaaa atatcactat
    13201 tgccagaaaa taccctatta gttttttcaa attcaggttc attatcttca tcagtctgct
    13261 acctttaata ccgctgttaa attcagatgg cttgctttac gctgaataat ataaaaacta
    13321 attaaactag aaaaaaatat caccataatc gaaataacat aggttttcag ttctataaca
    13381 aaaggaatct tgaaccaatc ggtttgcatt aattgcagta tagaccagga catcaaatac
    13441 ccgataacta atcctaaagg ggtagagatt attatttcaa agatgtgatt taaaaacagc
    13501 aaggtggata cttctccctg agtaaatcct aacacttgga gtgtcgttaa ctctctcgcc
    13561 ctttctgcca aggtgatgct cgcattatta taaacaacac tcaccgcaat aatgatggca
    13621 aaacttgcaa gaatggtagt aaatacaaga atgtgttttg caaaagtttc ctcaaatgtc
    13681 ctaatgatgg aggttttaaa agtaatagtg cctatcttgg gtatttcctt taccttttta
    13741 tataaatgtt tgacgtgttt ggtgtctact gtaatagccg ctgcattgac taaccgatcc
    13801 tcatttaata aacggttaag cgctttaatt tctatatagg caaacatacc tacatagtca
    13861 tttattactc cttgtacttt cagttgagtt tttgctttat ttccttccag cagttcgata
    13921 tcaagcctgt caccaacagc caaacgcaat cgttctgcca atccctcact gattaataaa
    13981 ccctgctcag gaatgatcat tcgattaaga tgcttgtcta ataaaatttt taattccgca
    14041 ttttcagcga taccaaacag cgaggataat tcactacgat gttggtaagt taatcgagca
    14101 ggaacaatac gataaccttc actgctcgta accccagtaa ttttattcaa ctccgttacc
    14161 gctattttat taacgggatt attaaagctg accatggcat gttcacgttg tgccattatg
    14221 aattgggtat tgatgagata gcgtatcgca tcttgccaaa acaaacccag aattatgata
    14281 gccattgcca acgcaatacc cacacaagtc aacatggtgc gtaaaaaatg gcggaaaata
    14341 tatcgataaa ccattttgac actactggga agtcttgaaa aaaatttaat tttttcaatc
    14401 aaagtggctc gatacacgat aggggcaggc ggtttcatcg ctactgcggg catgagattt
    14461 atgacctggt aaatcgcacg caatgtcccc aaaagagcag ccaaaaaaca aaccaggatc
    14521 ccgataaacg ctgctaaatt ggaaaaaaga taagagaacc tggggaacct gaaatactcg
    14581 gcatagagct cggtcatcaa attaccaaac caggcaccta atgacacacc tgcaagtgac
    14641 ccaataacaa taataattaa aattattttt atatagtggt aggcaatcgc caaatcacca
    14701 taacctaatg ccttcaaggt tgctatttgt tcgcgttctt tacttaccaa acgtcctgtc
    14761 accaaattca ataaaaacgc tgccaccaac agaaaaacag gcgggatata agtggcaata
    14821 attttttgct gttttatttc attggttaca aatcgatcgg atacctgatc ttttcgtgaa
    14881 tacgtgatgg tcaaaccata agaacgaaac attctttcca attgagtcat cacaaattgc
    14941 tctgacgcat taggcgcgag cttaatgatg atgtcattaa aagcgccctg catcccaaag
    15001 gtggtgctta atgcctttct atccatccaa aaaacaccga aatgcctgtt gtcaggaagt
    15061 aaatcttctc cccgaatggc gtacacatac tcaggtgata atgcaatccc aacaattttt
    15121 aactgttctc tgtgcccgtt taataatgca ataatgttat cgcctggttt taaatcatgg
    15181 ctgtttgcaa aactttcatt caccagtatc tcatcatatc gattgctgtc aggccatctt
    15241 ccctgacgta aatacaattg attaagaccc gatttattct tatccgatat cgatataaag
    15301 cggcctacag cgggctctgg catccaaggc aaatcaagca caacatcttt tacaattcgg
    15361 gtctctactc gagatactcc tgaaatttct gttattcgct tttctaaata gtcaggcgct
    15421 cttttcaatg aagcaaacac atctgcaaaa tgatattttg aataaaattc ttgttgtgca
    15481 ttccttaaag aaacataggt attgactgac gcaattaaaa cactgactcc actacagaca
    15541 actaatgcga tcgtgatgat ttgacttctt aatttgatca catcacgcca caattttttg
    15601 tttaatgctg ttaccattct aattcttctg gtttgcgctt gaatttattc ttggatatgt
    15661 tgcttatgac accatcagca atatggatca cacgatcagc catctcgctt atgacaatat
    15721 tatgggtaat aatgacaact gtcgtcttta atttttgatt cgccaaagca agtgtcgcta
    15781 aaacgatttt acctgtctca caatcgagtg cgcctgttgg ctcatcacat aagagaacat
    15841 caggattttt ggctattgcc cgagctattg cgacccgctg ttgttctcca ccggagagtt
    15901 gggcggggaa atgatgcata cgatgctcta aaccaagtaa tttaattgca tcttccgggt
    15961 gcatggggtt ttcagcaatt tcagtgacaa tagaaacatt ttccaaagcc gtaagattcg
    16021 aaatcaggtt ataaaattga aacacaaatc ctatatgctc acgacgatag cgagttaaat
    16081 catgttgact ggctgaggtg atgaggtgat ccttgtaaat tacttcaccc gatgaaggga
    16141 catctagccc tcctataatg ttcaataatg tggatttgcc acttcctgat gggcctaaaa
    16201 atacgaccaa ctctccctgg tacaggtcga gatttatgca tcgtaaagca tgaatgacaa
    16261 cttcacccat taaataggtt ttagtcaaat ttcgaataac aaatgctgga atcggtgaca
    16321 ttataaagtc ctttattaag ataagattcg aattgttatt ctcttatctt tacaatgcgc
    16381 ttcagcactg caacgcatta tcccgtcaga tcattccaaa aagagaaaaa cggagcagtt
    16441 tttatgtatt tttgttgtga tgatcattga acattccttg ttaatccatc ccagaaagct
    16501 ggttaataaa atgaagggaa gttaattggc actaccaacc atgccataag taatctattt
    16561 taaccgggtt tctctcaaaa gtcattttgc ctaatcatgt gttatatcat attgttcctt
    16621 aattattatt gagggtgagt ctgactaaaa gtctattgat caggacttac gaaaaatccc
    16681 aaggtcaagg caaaaaatgt tgctaataag ggagtttaga taaactaaat gaccgaatca
    16741 aaaacatttt taacaaagag cgtggggttt ttcgtaagtc atgttgatgt tgaattaacc
    16801 tggttgggaa taaagctttt tcaagaccac ttcaagagcc ttggcgctgg ctgaatcctg
    16861 gtcttcatgg gcgaaatttg acgccttttc caatgttatt gtggtcttgt taaacaaaca
    16921 atggcgtccg gatcggcctc caaaaaagcc tggttgataa ttggcaaaga aatgcttatg
    16981 gaaagttggg ggagacagga ggctaaacaa attctccatg gtataggtat catgagtgag
    17041 tctcccgacc aggaatcccc ccagatagaa catccaagga ctgttatcca gttttaatac
    17101 ggcgcgacct aatgagtcag ttggaaaatt ccactgcgtg tccatgggtt ttcgccctac
    17161 actgtttagg ttcgcttcaa tcagcgtgta tttaaaaaaa agagagcatg acagttgctg
    17221 ggcaaggcca ccagagattc ctgctcgaca agattcaagg ctcaatatct tgggcgcttc
    17281 ctttaaccca ttagcagtta atcgtctaat tagaccatct gctgtgtccc cctgcatata
    17341 caatccggta tcctctgatg gggttgactc aagtgaagaa tgtcccatga ttgtcagatg
    17401 atcttcgcat gtgacttgtc ctaattcctg atcgtttaat aagatataac catgatcctt
    17461 ggcattggtt tgctggccaa gcaaagttgc ctcagcagac tctaaaacat aggatggatg
    17521 aaaccagatg tatcgcttca tagaggaaat ctcttttatt aaacaagatg aactcaaacg
    17581 atacaaattt atttgttttt tgtaaatgca aaattcgata tttgttgtct tttcaaattt
    17641 aaaataatgt ccccagctca ggaaaataac ttgtgaagcc aagcttacaa gaaatgcttg
    17701 tccctgtttt tatagtggtg gcgcgcaaaa gccggggctg gcagcccccc tccccattgc
    17761 attgttccgg ctctttacag atacttccaa gttcctgggt gtcaaagcga aacgccttta
    17821 ttttgatggt ttttaacatt cactttttga gtcgactggt caaaaaagtt tggctgttgt
    17881 ccaaggtcag agttgcgatg gcctcttggg tcagtatggc acttcataaa gaaacctagg
    17941 cgatcaagac gctgattcat gttctttaat tccattttag agtagatccc ttgtctattt
    18001 tcaacttgat ttaacgccgt attgacagaa cgcaaatggc cgtagttgac tcttagtgtt
    18061 tttagtaaat cattgtagtg cacccgctcc aaagacgctt ccagcgatgc gaacaagtca
    18121 gcaaaaaatg attttgcact gagatatgga cgctgtggcg acctggccac agatggcttg
    18181 tcaactactg acgacccttg cattagttta ttgaataaac gcacatgacc attggtgcta
    18241 gcaacacaca ttaacatatt gtatcgttcg ctatagggaa gacacctgta gtttgcaacc
    18301 aaattcggga gctttttata caaaaccata catgaactta gcggatattt cgcaagctcg
    18361 ctatccagta aaccacattg tttcatttcg cataacaaaa aagctaattc tttgttgcga
    18421 aataattcac atcctatctc ggtcttatat tccaaaaaaa tttctgttaa taattttttt
    18481 tgtggagagc tgtcaaatac ggcaaaatga catttccttt tcctcaggtc agtgagcaga
    18541 caggtggttt gtttctgatt attccaggga ttgattaaag taacctcata ctctccatcg
    18601 tctaaaggct taatatttcc aacacgtagt gcatgacgcc tgtgcctgcc caatctatcc
    18661 agcttcccaa agttcatccc tacataaaca ggaatcatgg ggtctattgt tttaagctta
    18721 accagcttgt gcgggtcata gaaagaagtg acatgaactc ccaaaagctt ggctagaaat
    18781 gcagtatcgc tttgcttggc aataggatta ttgcgaaatt ttgggtgtag cttatgagcc
    18841 aaaagacttt cgccatccca ggcaatttgg tagtagtaag cgctgatgtg ctctaaaatt
    18901 tttactgcga gcgaattgga actggatcct tttttactgt tatcaatttc ttttaatttt
    18961 tcattatcga taaaaataac gtcttcatca gttctgggat cataagaata agtatacttc
    19021 ccagccattt tttcccactg caccttcatg gtgttgtcag tgcgtttaat tctaagagta
    19081 acgccggacg ttgtttgttt gaattttttc cgtacaacac cggccccttc tgcttcaccg
    19141 ttgagcaaac aatccaggca agccaggaga aagcaagacc cactcctgcc ttgttttatg
    19201 gctataggct ggcctttttg cggaaataat ggcgtcattg gttacaaata tataaattgc
    19261 ttcaggtgct ggatatgata accgaagact caaatttatc aaaataatgc gcacttagga
    19321 catttgcaag ccaaaaaaaa aacaataagg tagggaaaaa agttgagccc taaccacttt
    19381 atctgactta ttgtgaaatt gaaagtcgga tcaatatccg gggactcaac cctggtaatg
    19441 ggaaggttta agtgaaaaaa tccaccttga atttataact cacagctcag ccagagattt
    19501 gccccgtatt gagcacttat ttatccccat ctcaggagtt caaatcagat acaccaagcc
    19561 ataatagtct atcctatatg gtataggagt atgtgagaga tgccttaggc aatctcatta
    19621 tcatattaag ggataagaat ttatgaaact caccctattt ttagtattag tgtggatctg
    19681 cactgagtct tttgggacaa ctatcatggt aaaaggactg ccatctcctt tagagtacag
    19741 aaatgatctt tattattggc ctccaaatgt tgctatcagc cctgacacta ctaatttatt
    19801 cattacaatg gatggcatcg acaaggtgtg tttcttgaat acaggactcc aaggtgggtt
    19861 agagcaaatt tcagagatta gtattatcat caatggagca aaaaaggatt ggaactgttt
    19921 cccatatacc acgactattc ttgaggcacg cccatagaaa caccaaacca ccaatcgaat
    19981 aggtggcttg cccgtatcta ctcttgcttg gataagctca cattgtccct ttataaccgt
    20041 aaattgtcct tttaaaccat ggctcaattg attttttaaa gtacaattgt tatatatttt
    20101 tatcattaag gatttttcac tggctttttg attttaattt gcacccaaat aacctttaac
    20161 ctgaggatag ataatgaaac ctataaaaaa cagcaaagaa caagaatata ttgctcccga
    20221 agctcaagac cccagattga gctacactga ggacaatcgt cgcccactaa cgagcacaac
    20281 atcagcaaaa catcctagta gtttgttcgg caaatcggaa gagcgctcag caagggagag
    20341 ttacgatcaa tatggtaaca atgttagtac ccattggaca caaggtccct ggaattaatg
    20401 cactaactaa tggcttgggt cgtttccact caggccatgc atgccccaaa ttgcccttcc
    20461 tgtttaatcc attaatacta
//
LOCUS       DATNSN010000043        20022 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.043, whole genome shotgun sequence.
ACCESSION   DATNSN010000043 DATNSN010000000
VERSION     DATNSN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.043"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            135..1160
                     /locus_tag="VAH76_001311"
     CDS             135..1160
                     /locus_tag="VAH76_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO1385946.1"
                     /translation="MIELCGLKKSFSGKLALNDINLFIQEGEIFGVIGKSGAGKSTLL
                     RCINILEKPDEGEVIIDGQNLMSLSRKDLALARHKIAMIFQHFNLLNSKTVFDNIALP
                     MRIQGIDEESIKQKIEELLPVVELTDKKDAFPSQLSGGQKQRVAIARALSCSPKVLLC
                     DEATSALDPATTDAILSLLKKINELYGITIVLITHEMDVVKRICQRLSVMVDGKIVET
                     TALSNIFNKPESLARKMLYAQISPELPTCLTRRLADYATEKPLVRLFFQGEEATVPFI
                     SQTSRELNMDINILLANIDRFDGVTCGVLVVELTANPLLLEAFINRCEQAGITVEVLG
                     YVLPDGL"
     gene            1138..1785
                     /locus_tag="VAH76_001312"
     CDS             1138..1785
                     /locus_tag="VAH76_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1385947.1"
                     /translation="MSYQMVYDLLSATGETLYMVFFSTLFAVLLGLPLGILLYSSSRI
                     KPNIKLNKILSALINIFRSIPFIILLVAIIPFTRLIVGTSIGINAAIVPLTVGATPFF
                     ARLVDNVLQSLPLGLIETGYSMGANTRQIILHIILPEAQSGLIHSITVTAITLINYSA
                     MAGAVGAGGLGTLAINYGYQRFNAGIMIATVIVLIILVQLIQMVGDYLAKRCTHY"
     gene            1798..2577
                     /locus_tag="VAH76_001313"
     CDS             1798..2577
                     /locus_tag="VAH76_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO1385948.1"
                     /translation="MRILSCLVLIISLVACSSKPAPNTLVIGTIAGPETELIETAKQV
                     AEKEYGLNIKIVEFNDYNLPNEALQDGSLDANVYQHLPYLKAAILSHGYDLQAIGRTF
                     VYPMGIYSKKYKTLSELPENGIIAVPNDPSNEMRAFLLLEKAHLITLKNTTNSGIQDI
                     ESNPKQFKFKEIDAAQLPRVLPDVDAAVINTTFALPAGLSPSKDALFTEGKDSPYANI
                     IVIRRDTEKRPQLELFVKALNSEEVKEKAKKLFGEDAIPAW"
     gene            2657..4075
                     /gene="ceg25"
                     /locus_tag="VAH76_001314"
     CDS             2657..4075
                     /gene="ceg25"
                     /locus_tag="VAH76_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg25"
                     /protein_id="HEO1385949.1"
                     /translation="MAFYNSIPLIGKHLKSESRAKKYTSEEFKRLERKYRDHIGLPEN
                     SSFSSDAFDKLVSSRVDDFRAFPKTSLPGGLLFHDKLKEANTTEGENIRDVLSKTVLA
                     PTEKFKTAQKNFTESTSTFKELTKLIPSKFRAQDLVGTMKELTDDARKAIIEQQAHEI
                     QMLKEKLDPQKEGQAGNYVSDFKKALGLKDNDEVKKVTEGLIKELEETHKKQLGEFDK
                     STSDSLNQLHKASASESSEYLFLANLYENNKEMRQTLEKLYAKERQRKGLPPESTTVQ
                     VGISENKIDLNGIKLKDIPVIKSITGREIHQQPDGSFTIQMPMINPRYYQDPRQNVKT
                     DMMLIAQAIKASGYDSIEMNCNFPNSEKVAMERGRQAFAACIEAGFPVDKITINVNGK
                     PMKADELFKEHPKTYQDIMSRASKISKDYEDIKAPKTSTAPVNTVAVKQEIATLRAKQ
                     QQEERAQTQPVDEQRHGMTPNN"
     gene            4401..4568
                     /locus_tag="VAH76_001315"
     CDS             4401..4568
                     /locus_tag="VAH76_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385950.1"
                     /translation="MSTRRFDEFDNEMDDFSDAEPVITEASPSTKLERRRKIEDLLEE
                     KRLREELEEFG"
     gene            complement(4565..5296)
                     /locus_tag="VAH76_001316"
     CDS             complement(4565..5296)
                     /locus_tag="VAH76_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1385951.1"
                     /translation="MNRIKAVGYENNELKEKAQLLADQLNLQLDQNADTCLFVTPEKL
                     TLKIRNFSLMFADFSTMTWSKRRGEGKKQGLIRACKPTKGIKILDATAGWGKDAAILA
                     TFGADVLMLERHPVMAALLADALSRRNEADIQKMCLSLIASDAISFLHSLQEKDYPDI
                     IYIDPMHPERNKSALVKKEMQVLQQLIGTDYDAMELIKLSLSHVKSRVVVKWPQKVKP
                     LLPPDASIDGKTVRFDIYMPQFSSN"
     gene            complement(5299..5829)
                     /gene="gmhB"
                     /locus_tag="VAH76_001317"
     CDS             complement(5299..5829)
                     /gene="gmhB"
                     /locus_tag="VAH76_001317"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEO1385952.1"
                     /translation="MNRIILLDRDGVINTDSINYIKNVDELIIIPDSITAIARLSAAG
                     YKIGVATNQSGVSRGLYSEDDLSAIHCKLREVLAEAGGQIDALEYCIHLPEDNCFCRK
                     PQPGMLKALAEKLNCSLDSVPFVGDRVSDIQAALAVGAQPIIVLSNMTDMSELAQYPK
                     VPIYNSLLQYVNQLLL"
     gene            complement(5835..7901)
                     /locus_tag="VAH76_001318"
     CDS             complement(5835..7901)
                     /locus_tag="VAH76_001318"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO1385953.1"
                     /translation="MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEV
                     KRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAYDSAGNPTPALLGFAKSCGVTVD
                     ALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
                     HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFA
                     TRRQAIIHQVQELAAPLGASVVMPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALI
                     ASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQ
                     DKKQPLSAHIPSTEQVIFQVKLGSVYDKVQRIKVMMDHLSQSLNLPSHLAERATLLSK
                     CDLMTGMVGEFPELQGIMGYYYALNDGEEAVVATALNEQYMPRFSGDDLPSTDLGKAL
                     SLVDRMDTLVGIFAIGQKPTGVKDPFKLRRHALAVVRLLISISAPLNLSTLIKEALAN
                     YGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLDKRLFAL
                     KSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKT
                     VETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGV
                     ADISML"
     gene            complement(7891..8796)
                     /gene="glyQ"
                     /locus_tag="VAH76_001319"
     CDS             complement(7891..8796)
                     /gene="glyQ"
                     /locus_tag="VAH76_001319"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO1385954.1"
                     /translation="MTKPTTFQEIILTLQKYWSDLGCVILQPLDLEVGAGTFHPATFL
                     RAIGPEPWSAAYVQPSRRPTDGRYGENPNRVQHYYQFQVVLKPSPLDIQDRYLDSLRA
                     LGVDPLLDDIRFVEDNWESPTLGAWGLGWEVWQNGMEVSQFTYFQQVGGLVCKPITGE
                     LTYGLERIAMFVLGVDNLFDLPWCKTANGIVTYGDVFHQNEVEMSAYNFEHANVEELF
                     KQFDYYEGEAQKLIALQLPLPAYEMVIKASHTFNLLDARKAISVTERQRYILRVRHLS
                     RAVAQAYYQAREALGFPMQKVCNGQ"
     gene            complement(9031..9816)
                     /locus_tag="VAH76_001320"
     CDS             complement(9031..9816)
                     /locus_tag="VAH76_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEO1385955.1"
                     /translation="MKFTSLLTAGALASTLVSPAIMAADTASASLSDAQKKEIEKVIH
                     DYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLV
                     EFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQ
                     AMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLM
                     GTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN"
     gene            complement(10024..12189)
                     /gene="uvrD"
                     /locus_tag="VAH76_001321"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(10024..12189)
                     /gene="uvrD"
                     /locus_tag="VAH76_001321"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEO1385956.1"
                     /translation="MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIA
                     WLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHY
                     KEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
                     ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAIL
                     VDEFQDTNTIQYAWIRLLAGDHTAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQI
                     IRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILVYSAFNELDEARFVT
                     ERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTL
                     AYLRLLVNPNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGL
                     GQRGSLALAKFIDLIEKLQVVVANKELDEQISDVIQHSGLYAHFSKIKGDKSESRVDN
                     LQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTLHAAKGL
                     EFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGR
                     EEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFG
                     QGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL"
     gene            12410..13369
                     /gene="pip"
                     /locus_tag="VAH76_001322"
     CDS             12410..13369
                     /gene="pip"
                     /locus_tag="VAH76_001322"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEO1385957.1"
                     /translation="MHTLYPAIKPYTQHELKVSKLHTLYLEETGNPEGIPVIVLHHGP
                     GGEADPHLRRFFDPQRYRIILFDQRGCGRSIPHLEISENNTQLLMEDIDSIRDFLGLR
                     EFVLFGGGWGSLLALLYAQKFPQQVKALLLHQIFLGRQKDIDWFYKHGASLVYPDYWE
                     EFTNTVPENKLDNIPQYYAECLQGSNELARMAAAKNWALWQARCSSLQPHLYVIDQYS
                     DPHFALALATLESYYIINHYFIEENQVIANAHKIRHIPTYIVHGRFDLVSPLSSAWEL
                     HQAIPASNLRIVRDAGHSDRESGIIDALIYASKDISKQGLDAC"
     gene            13363..13800
                     /locus_tag="VAH76_001323"
     CDS             13363..13800
                     /locus_tag="VAH76_001323"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO1385958.1"
                     /translation="MLTVLQRVKEARVDIDGQTVGKINHGLLILCGFEPKDSLENIKR
                     MLDKCINYRIFEDPSGKMNLSLKDVNGGLLLVPQFTLMADTQKGLRPSFSNAASPELG
                     RELFDNLLTLAQKCHQNTQSGCFGANMQVYLCNDGPVTFLLQF"
     gene            14017..14799
                     /locus_tag="VAH76_001324"
     CDS             14017..14799
                     /locus_tag="VAH76_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEO1385959.1"
                     /translation="MRLILMFASIMGSVILASCASKGSNPIDPFEPFNRKVHNFNMAF
                     DATVLRPPAKLYQAVVPKLVRKGVNNAFNNLDLFPTIASDILQAEGKWVIKDSWRLFI
                     NSTIGVAGLFDVAEKFGLPPHYNDLGLTLAKWGDKKSPYLVIPFLGPSTLRDGAGWLF
                     QFALWSPYVYIDDAGLVYGLIGLRYIDLRTQFFDSEPLLNEALDKYTFIRDAYLQHRN
                     YLIAGTEQDTGTSFVEDKKANKTAMQESTASNNQLGSDYVDE"
     gene            complement(14908..15867)
                     /gene="gshB"
                     /locus_tag="VAH76_001325"
     CDS             complement(14908..15867)
                     /gene="gshB"
                     /locus_tag="VAH76_001325"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEO1385960.1"
                     /translation="MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYC
                     CDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYAL
                     DLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
                     PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGD
                     PVPFALARIPAKGDLRGNLAAGARGEVVAITERDKWICEQIAPALREKGLYFVGIDVI
                     GDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK"
     gene            complement(15867..17162)
                     /gene="gshA"
                     /locus_tag="VAH76_001326"
     CDS             complement(15867..17162)
                     /gene="gshA"
                     /locus_tag="VAH76_001326"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEO1385961.1"
                     /translation="MNKHEIPVPHLTTAHSGPLYHVEKIILNKLAEIESWFRKKWQET
                     PAPLTSSVDLRHAGFKLAPVDTNLFPAGFNNLNPEFLPLCIQAAQSALIEYIPDCTKI
                     LLLPESHTRNKYYLQSLNVLKTIFVKAGFIVRIGSLDPDIKEPTEIILEQGETLLIEP
                     LQRQGDKVGLDNFSPCLLLLNNDLSSGVPEILQDVQQRIRPTAKLGWASRLKSSHFQF
                     FEEVAIEFAELVGIDPWLINPYFSAIEGVDFMAQEGIETLAQEVDKILSLINDKYTTY
                     GIENKPFAVVKADNGTYGMSVMMVHNGDEIRQLNRKQRTRMSTSKGSRKVDRVIIQEG
                     VYTFETMPDGSVAEPVVYMIGQFVVGGFYRVHKGRGIDENLNAPGMHFEPLAFAQACN
                     MPCDDLEVVDCPNRFYVYGVIARLAALAAAREIAAIGGE"
     gene            17540..18436
                     /locus_tag="VAH76_001327"
     CDS             17540..18436
                     /locus_tag="VAH76_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEO1385962.1"
                     /translation="MLHFKTIQSILLLIVLGFIWGSGYTLAKFAMTNGVNPLGYAFWQ
                     SLGPALLLTLVSFFTKQTRLLHPVYWPYFFICGLIGIAIPNTNMYFIASHIPAGLLAV
                     LVNTVPLLVYPLALMAGQEKPDGWRFFALLLGMSGILAIIGINSAGLYSSWTILAMLS
                     PLGFAFCSIYIGAKQPKEINALQAANGMLLAASLLLTPVVLKQHAFYSLSAPLTLVKQ
                     IVILEIILSSLGYLLFFILIRMAGPVFYSLTGGVVAITGLFWGYLAFDEKPSSIQGMA
                     IALVVSAIFLLSWRQSKQTQGI"
     gene            18440..18721
                     /locus_tag="VAH76_001328"
     CDS             18440..18721
                     /locus_tag="VAH76_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385963.1"
                     /translation="MLSELAKQFSTQIQTFFYLIMLINGLLHFIFAGAVARDAGNLYR
                     LGQKPVLVSAPTWAFATLIGGVITATIYWLLHHSTLTRPTMREIRYDKA"
     gene            18708..19058
                     /locus_tag="VAH76_001329"
     CDS             18708..19058
                     /locus_tag="VAH76_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO1385964.1"
                     /translation="MTKHKIKTIDVHELKHQMDNQANLSLIDVRELDEWEMMHIPGAL
                     HIPKDRISLEIQNQIPNKEQTIYLHCRSGVRSLYAAQCLMDLGYYEVYSVDGGIMAWA
                     MSGYPVKQESYTPS"
     gene            complement(19082..19744)
                     /gene="lem14"
                     /locus_tag="VAH76_001330"
     CDS             complement(19082..19744)
                     /gene="lem14"
                     /locus_tag="VAH76_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem14"
                     /protein_id="HEO1385965.1"
                     /translation="MSFELVAYEKLKGSIRESIITLIKSHNEKAKIIEDKLEDSVKEV
                     SRERQPQVLVLLKTIELLDNSSKEPEDKARVLNALAYYIRDQIAATYKYTSPDNSDFY
                     KSLTISLDLNKDNNPNREDLADMYSALEKFLRSHVYKNSDPRKGYLDKQPFAIKHYSV
                     VDDILELSDRVHKLRHEIIIAARDLHLLQQKPKSSQGGLFSVPSGKEKVTDTHEVANH
                     RL"
BASE COUNT         6177 a         4146 c         3602 g         6097 t
ORIGIN      
        1 taaaatgcaa ttattgcttt ttattaagaa ttattgtttt ccagttcttc agatatcttt
       61 tgaaaatacg gctaaagcgg ttgctttcta atccccactt tatgccccgc aagccatatt
      121 tccaggttat agtaatgatt gaattatgtg ggctaaaaaa atctttctct ggtaaactcg
      181 ccttaaacga tattaattta tttattcagg aaggtgaaat ttttggagtt atagggaaaa
      241 gcggggcagg aaaatcaaca ttactgcgtt gcatcaatat attggaaaaa cctgacgaag
      301 gtgaagtcat tattgatggg cagaatctga tgagcctttc cagaaaagat ttagccttgg
      361 cacgtcataa aattgccatg atcttccaac actttaattt gctaaattca aaaactgttt
      421 ttgataacat agcccttccg atgagaattc aaggtataga cgaagagtcg atcaaacaga
      481 aaattgaaga gctgcttcct gttgtcgagt taacagataa gaaagatgct ttcccctcac
      541 aacttagtgg tgggcaaaaa caaagagtag caattgcgcg agcactaagt tgttctccca
      601 aagttctatt atgtgatgaa gccacttcag ccctcgatcc cgcaactact gacgccatat
      661 tatcactact aaaaaaaatc aatgaattgt atggaattac tattgtcttg attactcatg
      721 aaatggatgt cgtaaaacgt atatgccaaa gactgtctgt catggtcgat ggcaagattg
      781 ttgaaactac tgctttatct aatattttca ataagccaga gagtctcgct cgcaaaatgc
      841 tttacgcaca aattagccct gaactcccaa cctgccttac tcgcaggttg gcggactatg
      901 ctactgaaaa acctctcgtg agattatttt ttcagggcga ggaagcaacg gtacctttta
      961 ttagccaaac cagcagagaa ttaaacatgg acatcaatat tctactggca aatattgatc
     1021 gtttcgatgg agtgacttgt ggtgttttgg ttgttgagtt aacagcgaat cctttattgc
     1081 ttgaagcatt tatcaatcgt tgcgaacaag ctggtattac tgtggaggtt ttaggctatg
     1141 tcctaccaga tggtttatga tttacttagc gcaacaggag aaacacttta tatggtgttt
     1201 tttagtactc tatttgcagt cctccttggc ttgcccttag gtatattgct gtactcatca
     1261 tctcggataa aaccaaacat taaattgaat aaaattttat ctgctctgat taatattttc
     1321 cgttccatcc cgtttatcat tttacttgtc gctattattc catttaccag gctaattgtt
     1381 ggtaccagca tagggatcaa tgcagcaatc gtcccattaa cagttggtgc gactcctttt
     1441 tttgcaaggc tagttgataa tgttctccaa tcgctacccc ttggtcttat agaaaccggg
     1501 tattcaatgg gagctaacac acgccaaatt attttacata tcatattacc tgaggcacaa
     1561 tcaggattaa ttcattccat tacagtaaca gcaatcactc tgattaatta ttcagccatg
     1621 gcaggagctg tgggtgccgg aggattaggt acattagcga ttaactatgg ttatcaacgc
     1681 tttaatgctg gcattatgat agctactgtg attgtattaa ttattttggt ccaactgata
     1741 caaatggttg gagactactt agccaaacgt tgtacacatt attaattggg aggttcaatg
     1801 agaattttaa gttgtttggt tttaatcatt agcctggtag cttgcagtag taagccagct
     1861 cccaatactt tagtcatcgg cacaattgct ggccctgaaa cagaacttat tgaaacagca
     1921 aaacaagttg ctgaaaaaga gtatgggctc aacataaaaa ttgttgagtt taatgattat
     1981 aatttgccca atgaagcact gcaagatggc agtctggatg ctaatgttta ccaacattta
     2041 ccttatttaa aagccgctat actgtcccat ggttatgatt tgcaggcaat cggcagaacc
     2101 ttcgtttacc caatgggtat ttattcgaag aaatataaaa cactcagtga attacctgaa
     2161 aatggaataa ttgcagtccc taatgaccct agtaatgaga tgcgtgcctt cttattattg
     2221 gagaaagctc atttgattac tcttaaaaat acaacgaata gcggtattca agatatcgaa
     2281 agtaacccca aacaattcaa attcaaagag atagacgccg ctcaattacc cagagtactc
     2341 cctgatgttg acgccgcagt aattaacacg acgtttgcct tgcctgcggg attaagccca
     2401 tcaaaagatg ctttgtttac tgaaggcaag gattcacctt atgccaatat cattgtgata
     2461 cgccgtgaca ctgaaaaaag gccacagctg gaattgtttg tgaaggcatt gaattcagaa
     2521 gaagtaaaag agaaagcaaa aaaactgttt ggagaagatg caataccagc ctggtaatta
     2581 ttaataaatt agcttaattt ttacttaata aaaaactgtt atactttatc tagataacct
     2641 tattgaggta tttgatatgg ctttttataa ttctatccct ttaattggta aacatttaaa
     2701 atcagagagc cgtgcaaaaa aatacacttc tgaagaattt aaaaggctag agagaaaata
     2761 cagagatcac atcggtttgc cagaaaactc aagctttagt tctgacgcat tcgataagct
     2821 tgtcagctcc agagtggatg atttccgggc ttttccaaaa accagcctgc ccggggggtt
     2881 gctgtttcat gataaactga aggaagccaa tactaccgaa ggggaaaaca tccgggatgt
     2941 cttaagtaaa acagtcctgg caccaactga aaaatttaaa acagcgcaaa aaaattttac
     3001 agaatcaact tcaacattta aagagctcac caagttaatt ccaagtaaat tccgagccca
     3061 ggatcttgtt ggcaccatga aggaactgac tgatgatgct cgtaaagcga taatagaaca
     3121 gcaagcgcat gaaatccaaa tgttgaaaga aaaattggac cctcagaaag aaggacaagc
     3181 tggtaattat gtatccgatt ttaaaaaagc tctgggtctt aaggataatg acgaagttaa
     3241 gaaagttaca gaaggcttga ttaaggaatt ggaagaaacc cataaaaaac aactgggtga
     3301 attcgataaa tccacaagcg actcgttaaa ccaactacat aaagcctctg cgagtgaatc
     3361 atcagagtat ctgtttcttg ccaatctgta tgaaaacaat aaggaaatgc gtcaaacgct
     3421 tgaaaagtta tatgccaaag aacgccaaag aaaagggctt cctcctgaat ctacaacagt
     3481 tcaagtaggt atatcagaaa acaaaattga tctcaatgga atcaaactca aagatattcc
     3541 tgtcattaaa agtattacag ggagagagat tcatcaacaa ccggatggca gttttacaat
     3601 ccagatgccg atgataaatc cacgttacta tcaggatcca agacaaaatg tcaaaacaga
     3661 catgatgctc attgctcaag ctataaaagc ctcaggttat gactctattg aaatgaattg
     3721 caattttcca aatagtgaaa aggtagcgat ggaaaggggc aggcaagctt ttgccgcctg
     3781 tattgaagca ggctttcctg ttgacaagat caccattaat gttaacggca aacctatgaa
     3841 ggcggacgaa ttgtttaaag aacatccgaa aacatatcag gatattatgt caagagcctc
     3901 aaaaatctcg aaagattacg aggacatcaa agcgcccaaa acatcaactg ctccagtaaa
     3961 caccgtcgcc gtcaaacagg aaatcgccac attacgcgcc aaacagcagc aagaagaacg
     4021 agctcaaaca caacctgttg atgaacaacg acatggcatg acccccaata attgacttat
     4081 aagttgaatt tggattgttt aatccaaatt caacaaattc attatcctcc ccgaattttg
     4141 ctcaaaaaat ttcactacct caaaccatca atttctgata ttttatccat tctagacact
     4201 tgaattttaa atgacagatt ctttgtaccg ccacttatta gaagtatcca taaagcggat
     4261 accggttacc ttggataaac tgtgtcaata aggctatttg aggtggcatt taaaaataaa
     4321 gtgccagtat aactattcca ggtattttgg tatcatcgag aatagatatg gaattcaatg
     4381 ggttagctaa ggagaaagct atgagtactc gtcgctttga tgaatttgac aatgaaatgg
     4441 atgattttag tgatgctgaa cctgttataa ctgaggcaag cccttcaaca aaattggaaa
     4501 gacgtagaaa aatagaagat ctgttggagg aaaagcggct aagagaagaa ctggaagagt
     4561 ttggttagtt ggaagaaaat tgaggcatat aaatgtcaaa ccttacagtc ttaccatcaa
     4621 ttgaggcatc tggtgggagt aatggtttta ccttttgcgg ccacttgact acaactcttg
     4681 acttaacatg agataaagac aacttgatta actccattgc atcataatcc gtaccaatta
     4741 gttgctgcaa aacttgcatc tctttcttaa ccaaagccga tttgtttcgt tctgggtgca
     4801 tgggatcaat gtaaataata tcgggataat ccttttcttg caaactatgc aggaatgaaa
     4861 tagcgtcact tgcaatcaaa gacaagcaca ttttttgaat gtctgcttcg tttctacgag
     4921 acagtgcatc agcaagcagt gcagccatga ccgggtggcg ttccagcata agcacatcgg
     4981 caccaaaggt tgccaaaata gcggcatctt ttccccaacc tgctgtagcg tccaaaattt
     5041 taatgccttt tgttggcttg caagccctga tcaagccctg ttttttaccc tcccctcttc
     5101 ttttactcca ggtcatagta ctgaaatcag caaacataag agaaaaattt cgaattttta
     5161 aagttaattt ttcaggagta acaaaaaggc aggtatcagc attttgatct aactgtaaat
     5221 tcaattgatc agctaacaat tgtgcttttt ctttaagctc attattttca taaccaactg
     5281 cttttattct attcattctt acaataataa ttgattgaca tactgtaaca gggaattata
     5341 aattggtact ttaggatatt gtgccaactc agacatatca gtcatattag aaagtacaat
     5401 aataggctgg gcaccaactg ccaatgctgc ttgtatatct gaaaccctat ccccgacaaa
     5461 gggcacgcta tccagtgaac aatttaactt ttctgccaga gcctttagca ttcctggctg
     5521 gggttttcgg caaaaacagt tgtcttcagg taaatggata caatactcta aggcgtcaat
     5581 ttgcccccct gcttctgcaa gcacctctct caacttacaa tgaatggcag ataaatcatc
     5641 ctcagagtat agccctcttg aaactccaga ttgattggtt gcaacaccga ttttatatcc
     5701 ggcagcactt aagcgagcta tcgctgtaat gctatcgggt ataataataa gctcatcaac
     5761 attcttgata taattgattg agtcagtgtt aattactcca tctctatcaa gcaatataat
     5821 tcgattcata agtgctacaa cattgaaatg tcagctacac cctgcaataa ctcttgtaat
     5881 cgctgcagga gggcaagcct gttctgtttc aatgactcct cttcaaccat aaccatcaca
     5941 tgatcaaaaa aagcgtctac tggctcttta agactagcca gaagcttgag taatgcttgg
     6001 taatctccgg caatgtataa agtttctact gttttagtaa ttttattaag atgatcaaac
     6061 aatgcttttt cagcgccttc ctctataagc tcttccttaa tatctacttt tttatccgca
     6121 tatgctgcct gagccaagat attgccaaca cgcttacagg cagctgataa tgatgtcgcc
     6181 tctggcatag taataaatga ctttaaagcg aatagacgct tatctaaatc atataaccaa
     6241 tcatcctgac gagcacggac agcatgaacc aaatcagcac tgataccttg attttgataa
     6301 taagattgaa gcctctcaag aataaatggt tttaattcag ccactaaatt cttgtcttga
     6361 gggagtcgat caccataatt agccaaggct tctttaatca aagttgataa attaagcggt
     6421 gctgatattg aaatgagtaa acggactaca gccaaagcgt ggcgtcttaa tttaaaagga
     6481 tcttttactc cagttggctt ttggccaatc gcaaaaatac ccaccagagt atccatacga
     6541 tccaccaggg aaagtgcttt acccaaatca gtcgatggta aatcatcacc agaaaatcta
     6601 ggcatgtatt gctcatttaa agcggtagca acaacagctt cttcgccatc attaagagca
     6661 taataatatc ccataatccc ttgcaattca ggaaattcgc ctaccatacc tgtcatcaaa
     6721 tcacacttgc ttaataatgt agctcgctca gccaggtgag agggtaaatt gagagactgg
     6781 cttaaatgat ccatcattac ctttattcgt tgtaccttat catacacgct tcccagcttt
     6841 acttgaaaaa taacctgctc ggtagaaggt atatgagcgc ttagcggctg ttttttatct
     6901 tgtttaaaga aaaaggcagc atcacttaat cttgctctca ttaccttctc atttcctaaa
     6961 accacttgtt ttggattgga gctggcgata tttgataccg taataaaata aggtagcagc
     7021 tcaccatgct tattttttag tgcaaaacac ttctgatgag attgcatgga agcaataagg
     7081 gcttcagcag gaacctctaa aaattcctgt tcaaaatttg caatgagcgc ttgaggccat
     7141 tcgactattg aggtgacttc atcaaccaat tcttcaggca taaccactga agcacctaaa
     7201 ggtgccgcca attcttgtac ctgatggata atggcttgtc gcctagtagc aaaatcggca
     7261 attacaaaag catctttcat ctgatcttca tagcttgcag ctgaatttat tcttatttcc
     7321 tgcggatgat gaaaacgatg tcccctgcta tgacatccac ttgtaacgcc taaaatttta
     7381 tgattaagtg tctcacttcc ataaagcatc acggcccaat ggacaggtcg ggcaaattcg
     7441 tcgtctcctt ctccccatct catgggtttt gcgataggta aagactctaa agactgacta
     7501 accaatccag gcaagagatc ttttgtttta gtaccctcac tttgagtttc aaaaacaatc
     7561 cattcacctt tgtcagtttg agtcttggtg agcgcatcta ccgtaacacc acatgatttt
     7621 gcaaatccta acaaagcagg agtcgggtta cctgcactat cgtatgctgc tattgcagca
     7681 ggaccgcgtc gcatgacttt ttggctttcc tgttcagttt gtaagtcatg aatcacaata
     7741 gccagacgtc ttggtgtagc gaatctcttg acttcaccat aacttaattg cgccttatct
     7801 aatgcggcta acaatcgagt agaaaactca tttgccaaag gccaaacagc accagaaggc
     7861 agctcttcac atcctaattc aaaaataaaa tcattgacca ttacacacct tttgcatagg
     7921 gaaacctaac gcctcgcgcg cttgatagta agcttgagct actgctcttg atagatgtct
     7981 tacacgtaat atataccttt gccgctctgt cacagaaatt gctttacgtg catccagtaa
     8041 gttaaaagtg tgagaagctt ttattaccat ttcgtaagca ggaagaggta attgcaaagc
     8101 tatcaatttt tgtgcctctc cttcataata atcaaattgc ttaaataatt cttcaacatt
     8161 ggcatgttca aaattataag ctgacatctc tacttcattt tgatggaata catccccata
     8221 tgtcactata ccattagctg ttttacacca aggcaaatca aataagttat ctacccctaa
     8281 aacaaacatt gcgatacgtt ccaaaccata ggttaattca ccagtgattg gtttacatac
     8341 aagtccacct acttgttgaa aataagtaaa ctgagacacc tccattccat tttgccaaac
     8401 ctcccaacct aagccccatg cgcctagagt tggtgactcc caattgtctt cgacaaaacg
     8461 gatatcatcc aataagggat ctactccaag tgcgcgtaac gaatccaagt atctgtcttg
     8521 tatatccaat ggcgacggtt ttaataccac ctgaaattga taataatgct gaacccggtt
     8581 tggattttct ccataacggc catccgtcgg ccttctggat ggctgaacat aagctgctga
     8641 ccatggctca ggtccaatcg ctcttaaaaa agtagctgga tgaaaggtac ccgcgccaac
     8701 ttccagatcc aagggttgca aaataacaca accaagatct gaccaatatt tttgcaaggt
     8761 cagtataatt tcctgaaaag tagtaggttt agtcatgaaa atctcaaaaa acaaatatga
     8821 tctttaaata taggttggtt tttaatcctt ttatccagga tatatcccat aaaacctatc
     8881 tttcatttag agaattaatt attcagtcca ataaattatt ggattgttgg gtgccagggt
     8941 tgattgaact caacgccaca acactaaaat actatcccag ttgaggacaa ctgggatata
     9001 tgaatttaat ccaagtattc agataaatac ttaattgcca gccgcctttt taattaactc
     9061 acgtagtgat tgctcgcttg tagcgcctgg tataaatgat atttcactgc cctttttata
     9121 ttgaccatct ggtgttgagc caattataaa ggctggtgta cccatcaaat gtaatttttc
     9181 agccagttgg cgattcgcat ccaggatatc ggtcacttcc tgactatcca tatctttttt
     9241 cagtttttgc atatccagac ctatggattt ggcggcatcc atcactgttt tttcatccaa
     9301 acgtttgtct atcgtaatca aagcattatg catagcctga tatttgcctt gcatgccagc
     9361 cgccagagca actctggaag ccagatcaga agtcttgcca aagattggaa attctttgta
     9421 tataactcgt aatccgctgt cttttttaac cagattttca attgtagatg ccattttttt
     9481 gcaatgaatg cattgataat caaaaaactc aaccagtgtt acattccctt taggattacc
     9541 tacagtagtt aatttgcctt gaaaaacctg ttcagcattt tcctgaattg cggcttgagc
     9601 ttgctgttgc atattttgct gctgtttttg ttgtaatgct tgcgaagcct ccaataaaac
     9661 ttccgggtta ttaatcagat aatcatgaat gactttttca atttcctttt tttgggcatc
     9721 tgataaagaa gcgctagccg tatcagccgc cattattgca ggtgatacta atgtagaggc
     9781 taatgctcca gctgttaata aacttgtaaa tttcacgtaa ttccccttac ttataattaa
     9841 tcaatgacgc aaattacatc tcgcagataa ctttatcatt aattgatctg ctcaaaacag
     9901 caaagtataa caaatttctg ttgcctaacg caccctagca ttcatgaaaa aaaaattcaa
     9961 tattacaaca acaaactgca agtcagggta cctcagatat tttataaaaa ttaaatgcaa
    10021 taactacagg cattcagtca aattagcata ggccaaaacc aaccatttta caccatgttc
    10081 actgaatttt acttgaactc ttgtatgggc cccgctgccc tcaatggcaa gaacgacacc
    10141 ctgtccaaat ttggcatgct gcacattttg cccaattgta atgcccgaca cctcgtcagc
    10201 cactggcaat ttgctttttg tagttccggg ccactgagaa cgtgatttaa ctcgtacctc
    10261 atcaagaaat tgttgaggaa tttcacgcaa aaatcttgat gatctgtgat actcttcacg
    10321 gccatattgt cttctgacct cagcatagga taaaaccaat ttacgcatgg cacgagtcat
    10381 accgacataa cataatcgac gctcttcctc caagcgacca ggctcttcaa tcgattgtct
    10441 gcctggaaat attccttctt ccataccaac caaaaacaca atcggaaact caagcccttt
    10501 ggctgcatgt aaggtcatca agtgaacgga gcgctcatgt tcatcagcct gcaattcccc
    10561 cgattctaat gaagcatgag caaggaatga attgaccaac ggtaactcct cctcttcatc
    10621 gtactcatat cgaaattgtt tggcagcatt aattaattct tgcaggttat ccacacgtga
    10681 ttctgattta tcacctttga ttttgctaaa gtgggcgtat aaaccactat gctgtattac
    10741 atcagagatc tgctcatcca actccttatt agcaaccaca acctgtaatt tttcgatcaa
    10801 atcaataaac ttagctaatg ccaagctccc ccgttgccct aatcctgtgg attgaagtat
    10861 gcccttggaa gcgtcccaca acgaacactg ttctgctctg gcgtactggc gaacttcatc
    10921 caatgttttt tctcctattc ctcgagtagg aaaattaacc actctttcaa acgcagtatc
    10981 gtcatttgga ttaactaaca atcgtaagta agccaaagta tctttaattt ctgccctgtc
    11041 aaagaaacgg actcctccat atatcctgta ggctatacct acacgtaaca aagcttcttc
    11101 caaaacacgg gattgggcat tcgaacgata caacactgca atctcatccg cactggcgcc
    11161 ctgattcaat tccatgccaa ttcgttcagt aacaaaacgt gcctcatcca attcattgaa
    11221 tgcactgtat acaagaattt tttctccttc actaccagca gtccataact ctttacccat
    11281 acgagtactg ttgttgtgaa tcaatgcatt tgctgcattt aatatcattg cagttgacct
    11341 gtaattttgt tcaagtctta tgatttgggt atccttaaaa tcatgaacaa attgttgaat
    11401 attttccact ttagccccac gccaaccata aattgactga tcatcatctc ctacagccaa
    11461 taccgctgta tgatcaccag caagcaaacg gatccatgca tattgaatcg tattggtatc
    11521 ctgaaattca tcaactaaaa tagcttggaa gcgttcacgg taatgagcaa gaatctcttc
    11581 attatctcgt agcaattcat gagttcgtaa taatagttca gcgaaatcga ttacccctga
    11641 ggtttgacaa acgtcttcat acgctttata aattgaaacc aaagtcttgg tcgggccata
    11701 atgcagtgca ttaatatgtt gtgggcgcaa gccttcatcc tttttactat taataaatgc
    11761 ttgcgcttgt ttgacttgcc attgctcagg atcaagatta agtgaaagaa tgactcgctt
    11821 gataactcgt gcctggtctt ccgaatccag aatatgaaac tgttctggaa ggttagcctc
    11881 cttgtagtgg cgacgcaata aacgatggca taatccatga aaagtaccaa cccataaacc
    11941 caacgttggt gtagacaaca tactgctaag tctcgatctc atttcaccgg ccgctttatt
    12001 cgtaaatgtt actgctaata tagcgtgggg agagagatgc tgttcttcaa ttaaccaggc
    12061 aattcggctc accaagactt ttgttttacc actgcctgca cctgctaaaa ccagcatatt
    12121 acctaatgga gaggttaccg cgtcacgttg cttttcgttt aatcccttga gaagtgctgc
    12181 tatggtcata gaatatttcc aacagaaaaa gtgcaagtat tatcttcaat ttaattgcta
    12241 aagcaaagat ataatcgata cggtaaaaaa tctaaaaatt ctcctggata tatatcgata
    12301 aaacccatac aagtattgct ttctgatcaa tcagagcgca aaatactgtt cgtttaaagt
    12361 agctactaga tagtattata atacaatgac gttatcataa aggactaaaa tgcataccct
    12421 ctacccagca attaaacctt acacacaaca cgaattgaag gttagtaaac tgcatacact
    12481 ttatttggaa gaaaccggaa atcctgaagg catacctgtt attgtattgc accatggccc
    12541 aggtggagaa gctgacccgc atctaagacg tttttttgat cctcaacgat atcgcattat
    12601 tctttttgat cagcgaggtt gtggccgttc aattccacat ttggaaataa gtgaaaataa
    12661 tacccagctt ctcatggagg atatcgactc cattagagat ttcctgggct taagggaatt
    12721 tgtgttattt gggggtggat ggggttctct gttagcatta ttgtatgcgc aaaaatttcc
    12781 tcaacaggtg aaagcattgc tactccacca aattttttta ggtcgacaaa aagacatcga
    12841 ttggttttat aagcatggag ccagtctggt ttatcctgat tactgggaag agtttacgaa
    12901 cacagtcccc gaaaacaaac ttgataatat cccccaatat tatgcggaat gtctacaagg
    12961 ctctaatgag ctagcgcgaa tggctgctgc gaaaaactgg gctctttggc aagccagatg
    13021 cagtagttta cagccacact tatatgttat tgatcagtat agcgacccac atttcgcatt
    13081 agcattagca actctggaat cctattacat tattaatcat tattttattg aagaaaatca
    13141 ggttatagct aatgcccata aaataaggca cattcctacc tatattgttc atggccgttt
    13201 tgacttggta agccctttat ccagcgcatg ggaacttcat caagcgatcc ccgcttccaa
    13261 tctaagaatt gtcagagatg ccggtcactc tgatagagaa tcgggaatta ttgatgcact
    13321 tatttatgcc agtaaggaca tctcaaagca gggtcttgat gcatgttaac tgtattacaa
    13381 agagtaaagg aagccagggt tgatattgat ggtcaaacag ttggcaaaat aaatcatggc
    13441 ttgctgatac tttgtggttt tgaacctaag gattcgctag aaaatattaa acgaatgctg
    13501 gataaatgca ttaattatcg catttttgaa gacccgagtg gcaaaatgaa cttgagttta
    13561 aaggatgtaa atggcggctt gctattagta ccacaattta ctctcatggc agatacccaa
    13621 aaaggattgc gtccaagttt ttcaaatgca gcttctccag aactcgggcg tgagcttttt
    13681 gataacttat taactcttgc tcagaaatgc caccaaaata ctcaaagtgg ttgttttgga
    13741 gcgaacatgc aagtttattt atgcaatgat gggcctgtga catttctgtt acaattttaa
    13801 ttagttttta ctgtattaaa tttttgtata acctgaattg tcgagcaaaa tacaatcaac
    13861 attcattatt ggaattcatt ccggccaaaa aaaagcatgt ttgtttcagt taaaaagcag
    13921 caatttaaaa ggcctattct ttacatcaaa gcatttgttt gcaagaacca aaaactggtg
    13981 taacatcatt ttaagtattt aataaataga ataattatgc gactaatctt aatgtttgct
    14041 agcatcatgg gatccgttat tttagcgagc tgtgcaagta aaggatcaaa cccgatcgat
    14101 ccattcgaac cctttaatcg taaagtgcac aattttaata tggcatttga tgctaccgta
    14161 ttaagaccac cagcaaaatt atatcaggct gtcgtaccaa aattggtaag aaaaggtgtg
    14221 aataatgcat tcaataatct ggatttgttc cctacaatag ccagcgatat actgcaagct
    14281 gaaggaaaat gggtaatcaa agacagttgg cgactcttca ttaactccac tattggggtt
    14341 gctggtctgt ttgatgtagc agaaaaattt ggtttgccac cgcattacaa tgatctgggc
    14401 ctaacactcg ccaaatgggg cgataaaaaa tcgccttacc ttgtaatacc ctttttaggc
    14461 cccagtactc ttcgtgatgg agccggctgg ctgttccagt ttgcactctg gagtccttat
    14521 gtatatattg atgatgctgg tctggtttat ggtttaattg gattacgcta tattgattta
    14581 cgaacacaat ttttcgactc tgagccttta ctgaatgaag cattagacaa atacacattc
    14641 atcagagacg cttacctgca acatagaaat tatcttattg cagggactga gcaagataca
    14701 ggcacgtcct tcgttgagga taagaaagct aacaaaactg caatgcaaga atcaaccgct
    14761 tcaaataacc aactaggttc tgactatgtt gatgaataaa atgattaatt gagacttgcc
    14821 aggtcatgat aagggttggt gttgagccaa atgcaaaacc aaccctcttt aagctctgaa
    14881 cttcaaaatc aaaataatga ctgttcttta ttttgataaa gaagtatgta acttttcaag
    14941 gcagcgcata tattcacctg cgatatccaa accagtttca gtgctaattt cccgaataca
    15001 agtgggacta gtcacattaa tctctgttag ataatcacct atcacgtcga tacccacaaa
    15061 atacagacct ttttctctca aagctggagc tatttgttca catatccatt tgtctctttc
    15121 agtaatagct accacttctc ccctggctcc tgcggccaaa tttccccgca aatctccttt
    15181 tgcaggaata cgagccaaag caaaaggaac ggggtcgcca ttgatcaata aaatgcgttt
    15241 gtctccgctt actgtaatat ccgggatata acgctgagcc ataatacatt ttgtctgccc
    15301 tttggttaat acctccaaaa tgaccgataa atttttacct tctttgtcaa cataaaaaac
    15361 ggaattacct cccattcctt caagaggttt aaaaataact tgttgatgaa tttgccagaa
    15421 ctcttttaat tgccgagcat ctctggatac aaaagtaggg ggacagcaat gaggaaaatt
    15481 tagggtaaaa aatttctcat tcgcgtcacg taaactttgc ggtttatttg caaccaacac
    15541 cccttcttgt tccgctaaat ccaaagcgta agttgcataa atatactcca tatcaaatgg
    15601 aggatcctta cgcatcaaaa taacatcaaa atctctcaat ggcttattac ccaaatctat
    15661 ggttttaacc caactcagac tctctatatc tccaatgtga atttcatgga ctcgtgcata
    15721 agcgccccca tcacaacaat ataagtcatt cagggtaaag taggcgcaag accatcccaa
    15781 tccttgtgcg cttttaatca tagctactgt agagtcttta tagggtttta tttgatccaa
    15841 tggatccata agcaccgcaa gtttcatcat tcgcctccaa ttgcagcaat ttctcgagct
    15901 gcagccagtg cagccaaacg agctataacc ccataaacat aaaaacgatt tgggcagtca
    15961 actacctcta aatcatcaca tggcatatta caagcctgtg cgaaagctaa cggctcaaaa
    16021 tgcatgcctg gagcattgag gttctcatca attcctctgc ctttatgaac gcgataaaat
    16081 ccaccaacaa caaattgacc aatcatataa actacaggtt cagcaacaga accatcaggc
    16141 atcgtttcaa aagtataaac accttcctga ataatgactc tgtctacctt cctgcttcct
    16201 ttactggtag acatcctggt acgctgcttt ctattcaatt gtcgaatttc atctccatta
    16261 tgaaccatca taacgctcat gccataagta ccattatctg cctttactac tgcgaacggt
    16321 ttattttcta ttccgtaagt agtatattta tcgtttatta acgataatat cttatcgact
    16381 tcttgagcta aggtttcaat cccttcctgg gccataaaat cgaccccctc tatagcgcta
    16441 aaatagggat taattaacca cggatcaata ccgactaatt cagcaaactc aatagctact
    16501 tcttcaaaaa attgaaaatg actcgatttt aatcgagaag cccatcctaa tttggctgta
    16561 ggtctaattc gctgttgcac atcctgtaat atttcaggaa cgcctgaaga caaatcgtta
    16621 ttcaataaca aaaggcaagg gctgaaatta tccagtccca ccttatcacc ttgtctttgt
    16681 aatggttcaa ttaataaagt ttcaccttgt tcgagtatta tttctgtagg ttctttaatg
    16741 tctggatcta aactgcctat tcgaactata aaaccagctt ttacaaaaat tgtttttaat
    16801 acattcaaac tctgtaaata atacttattc cgagtatggc tttctgggag aagcaaaatt
    16861 ttagtacaat cagggatata ctctatcaag gcagattgag cagcttgtat acacagggga
    16921 aggaactccg ggtttaaatt attaaaacca gctggaaata aattggtatc gacgggagcc
    16981 aatttaaagc cagcatgtcg caaatcaaca gatgacgtta gaggtgccgg tgtttcctgc
    17041 cacttttttc tgaaccagga ttctatttca gcaagtttat ttaatattat tttttccaca
    17101 tgatacaaag gacctgaatg agcagtagtc agatgtggaa cggggatttc atgcttattc
    17161 attcatactc cctatttttt aaaatggatc gatgttacct aggttattca taaaataaaa
    17221 gtatttatca ttcttaatac attattacta cagttttcat gatgacaaca tatatagaaa
    17281 agagaggcat ttatataata gtcgtgtttc acaacaacat attgtgaata cagcaatatt
    17341 tattccatca tcgattcttc atgttcttga ccaggatttt tggcaaaatg tactgcaatc
    17401 atataaaata ataattcagc atttgtaggg attaaaaaac tgttttattg agcaaaattt
    17461 aaaggcctca caagaatatc gttattttct actttcaatg gcataattat ccaaaattat
    17521 actatttatc ctacctttta tgctccattt taaaaccata caatcaatcc ttcttctaat
    17581 tgttttagga ttcatctggg gatcaggcta tactcttgcc aaatttgcaa tgactaatgg
    17641 agttaaccca ctaggctatg ctttttggca atcattaggg ccagccctac tattaacttt
    17701 agtgagcttc tttacaaaac aaactcgctt actacaccct gtttattggc cttatttttt
    17761 tatatgtgga ttgataggta tcgctattcc taataccaac atgtatttta ttgccagcca
    17821 tatcccggca ggtttattgg cagtgttagt taatactgtt cctttactgg tttacccttt
    17881 agctctcatg gcgggccaag aaaaaccgga tgggtggcga ttttttgcct tgttacttgg
    17941 tatgtccggc atacttgcaa ttataggaat aaactccgca ggactttact caagctggac
    18001 tatattagct atgcttagcc ctcttggctt tgctttttgc tcaatttata taggagccaa
    18061 acagccaaaa gaaattaacg ccctgcaagc tgctaatgga atgttactgg cggcgtctct
    18121 tctgcttacc cctgttgtcc tcaagcagca cgcattttat tctctctcag ctccgttaac
    18181 tctcgtgaaa caaatcgtta tccttgaaat aattttatcc agtttaggat atttactttt
    18241 ctttattctc attcgtatgg caggacctgt tttttatagt ttaactggtg gagtcgttgc
    18301 aataaccgga ttgttttggg ggtacttggc ttttgatgag aagccttcat ctatacaggg
    18361 catggcaata gctttagtgg tttccgccat ttttctgtta tcatggagac aatcgaaaca
    18421 gactcaagga atctgattca tgctcagtga attagcaaaa caatttagca cacaaatcca
    18481 aacctttttc tatttaatta tgttaattaa tggtctttta cattttattt ttgccggagc
    18541 agtggctcga gacgcaggta atctctatag gctaggtcaa aaacctgttt tagtatcggc
    18601 ccctacctgg gcatttgcga ctctaattgg aggagtcatc acagcaacta tatactggct
    18661 gcttcatcac tccacattaa ccagacccac tatgagagaa attcgttatg acaaagcata
    18721 agatcaaaac aatagacgtt catgaactaa aacaccaaat ggacaatcaa gccaatttga
    18781 gtttgattga tgtacgtgaa ctggacgaat gggaaatgat gcatatcccg ggagcacttc
    18841 atattcctaa agatcgcatc tctcttgaaa tacaaaacca aatacccaat aaagaacaaa
    18901 ctatctattt gcattgccga agcggcgtca gatcactgta tgcagctcaa tgcttaatgg
    18961 atttgggata ttacgaagtc tattctgtgg atggtggaat tatggcttgg gcaatgagtg
    19021 gttatccggt aaaacaggag tcttacaccc cttcctgagt gaagaggtga gagtagaaag
    19081 cttaaaggcg atgatttgca acctcatgag tatcggtgac tttttctttt cctgatggaa
    19141 cactaaaaag accgccttga gagctctttg gtttttgttg cagtaaatgc aaatcgcgag
    19201 cggcgataat aatctcatgt cttaatttat gcactctatc agataattca agaatatcat
    19261 caacaacgga ataatgcttg attgcgaaag gttgtttatc caaataccct tttcttgggt
    19321 cagaattttt ataaacatgg gaacgaagga atttttccaa agcactgtac atatcagcca
    19381 aatcttcccg attaggatta ttatccttat tcaaatcaag cgatatggta agagatttat
    19441 aaaaatcact gttgtcggga gaggtgtatt tataagttgc agcaatctga tcacgaatgt
    19501 aataggccaa tgcatttaat acgcgtgcct tatcttctgg ctctttggat gagttatcca
    19561 gcagttcgat agtttttagc aagacaagga cttgtggttg cctttctctt gaaacttctt
    19621 ttactgagtc ctctaatttg tcctcaataa ttttggcttt ctcattatgg gacttgatta
    19681 aagtaatgat actttccctg atgcttcctt ttaatttttc atatgcgacc aattcaaatg
    19741 acatcttatt ttctccttat actccatgaa caggactagt gaaatgcttt cactgtaggc
    19801 cagtttaaac caattctctc ttttcgtcat gctttttctt tttactatct aattaatttt
    19861 caatctattg attttaatga aaaatttgta atttccttgt tagttcatca aatgaataga
    19921 cttctggaga gtatttttaa gccttgttat cgtcgcttta agagtaagct cagatcaatt
    19981 aaatcaagat attttttgct caatcagtca gacacaataa aa
//
LOCUS       DATNSN010000044        20009 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.044, whole genome shotgun sequence.
ACCESSION   DATNSN010000044 DATNSN010000000
VERSION     DATNSN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.044"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            12..218
                     /locus_tag="VAH76_001331"
     CDS             12..218
                     /locus_tag="VAH76_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO1385966.1"
                     /translation="MSEKIRGTVKWFNESKGFGFLESGGKDYFVHFSAIQGSGFKTLA
                     EGATVMFKACNGQKGPQAEEVEVV"
     gene            complement(478..2208)
                     /gene="ceg34"
                     /locus_tag="VAH76_001332"
     CDS             complement(478..2208)
                     /gene="ceg34"
                     /locus_tag="VAH76_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg34"
                     /protein_id="HEO1385967.1"
                     /translation="MLMIMARIIVTYSPEVGGSSVLSLKESFTYVGSEVIDGDYRKMM
                     EDIPKEEFGNLYSTKEGRQRLFAHAKAKAMELLKNVDVLALSGNNSMIDPELFNQTRD
                     PDQNYDFSRTIAELALAHVAIEKGMPILGVCGGHQVIAVYGGGEICDLNSDKLNKQQV
                     MNYDAIKLHKSSLIAQIIGGKQVQKEMDTARKAFNERMNQYNRLSEKINALEKLNIVQ
                     EAGKLDALGKLEELDKSEVQLLLKLTGLNIEEELFDLRELIGEMGESDQLKTELQNIK
                     TSLNVEDSHSLNSPHYETEFFGAHEQVVSKLAAGFQHTGTASDNESIEAAESEFGVPI
                     ITTQFHPEVGAKGLPEAHFFYLRTEAEQEMNLNIFRYLDKAGDAYNKKKNLMVELKKI
                     NPKNQDALLQPSQIKKTSKERQQEKEKQGNKSSSTRQTESYFFESILSKIGSYLSAIG
                     EFIKEGIGKFLRDILSSMFTYDSVSRLKKKEQKQSKSEHSKIDLIDSKSNSYSTVFHD
                     MKVKSPSEILRENSAKLWAKKDNNHEKTITNQTDMNNSNKVRITTELVQEEVGQEFDE
                     NHTGLYRNNL"
     gene            2649..3533
                     /locus_tag="VAH76_001333"
     CDS             2649..3533
                     /locus_tag="VAH76_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385968.1"
                     /translation="MVPPEGFCFNYETSTSNSFQSFLSIKDIGNKALAEFTAMVLQLE
                     KEEIKVLTLSQSRDLPDAVFPNNWFSTHIDARGNNILIIYPLLAKNRQAEVNIKGLME
                     VLNRAQFEVHEIIDLRNDKDEILEGTGSLVLDRENRLLYASLSPRTSLNLVNKVADIL
                     GYTPIVFNSVDRQNKPVYHTNVIMGLSKCYAIICLDSINDAQQKAILSESFKETGKTL
                     IDISWNQVQHMCGNVLELYNKKGESLLILSEQAKRHFTHEQLRIIQQYSRLISTDIPT
                     IEAIGGGSARCMMAEIFY"
     gene            3680..4936
                     /locus_tag="VAH76_001334"
     CDS             3680..4936
                     /locus_tag="VAH76_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2828 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385969.1"
                     /translation="MKISELKKLVAELNKEVSPKVECTNIINLAGNLSEIIEYFEQDE
                     HASPELIYRIKAVICEFWKLVSLTLPYEEWQSSIQVAPWLTLQQSLSKAGLLPTDFHH
                     PILYQRLKEHYESFSHSELGIDQLLPLLIRCSRMTGYANKDPESLDTYPHSPLNKQIE
                     ARRPQELAKLKDILCLLRAIFYLIHHCCTIEQLTLIPYLIYFRNPTTDEERRSELAIF
                     NWLIQKPADCLEFFKTNEDYIDTRSFRQINELAPLRPFIPTARSDFIKITNKEHWIYP
                     FIQSRTNTSRSEYDLLNDAVNWLDTDFATEKDKSYHAALEFAHTVKKQANILTQREMK
                     IVHSALYVFCLDKYIKHRKADPRPRCTPFSLSGETKCSAAEKKQQEILEKPTKFGFFE
                     NLALNEGRLKTLTKTFEMPSYTLPGN"
     gene            complement(5047..6687)
                     /locus_tag="VAH76_001335"
     CDS             complement(5047..6687)
                     /locus_tag="VAH76_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2832 family Dot/Icm T4SS effector"
                     /protein_id="HEO1385970.1"
                     /translation="MTNKTQELQRVIADMAVLIEQDAEPDPQLYAFFFQHPEISLELI
                     HLINNLSEELVHDGPPIYSACVFALDICIAQLQAAAEANNKNATKLLSQLMNHLAEVI
                     DTHKHSLSFWLPVLNAFYEVHVELTDDLKEAYYGLASQDDDAVEVDEQFNHLDSIKDL
                     IHELSDLTLFDIAESFFAQSYAMPPDFFADLIVDLYSIEEGKDIALLVLLHPNIEVRE
                     IALSTIDQLMNTITLTPASLSRLQIIKHWYPTHYHGMFDRWIKTQRKKGVTFETEASQ
                     AKITIKATEVDGSGSQGVFIHARKNKKNRLCGLLLKYDLGIKDAWITPEISAAEVTDY
                     YHKAFEDSVTLREVDYSYFQTIVEHFLAITVEKSDIPNLHFLEILEFLGIRLKPDKLD
                     LDCVFEQLSVQIIPFTQEKIAESLKRSKMWLKNKQFTESWYIENPTIDKIVNHNSSFV
                     EGVKVCRLADAMDAVFSEEMELHRDKWQFHFLWIALWVKAKHRKNEKVWQDSFLIAYA
                     IHQGYPLKDIPVMKEICYQTVVNSIETMQERRTHLSQE"
     gene            complement(6684..7064)
                     /locus_tag="VAH76_001336"
     CDS             complement(6684..7064)
                     /locus_tag="VAH76_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEO1385971.1"
                     /translation="MQTPKGELTIQTLAMPLNTNANGDIFGGWIVSQMDLAAGVLAKR
                     ISHGRVATVAINSMTFLKPVHVGDVVSCHVELVKVGNTSMTIRVEVWAYSSIKMEKYQ
                     VTEGIFVFVAIDENGKPRQVPKTQ"
     gene            complement(7067..9436)
                     /locus_tag="VAH76_001337"
     CDS             complement(7067..9436)
                     /locus_tag="VAH76_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO1385972.1"
                     /translation="MAEVMLASAAIIADELKVKVSQVETAIRLLDEGATVPFIARYRK
                     EATGGLDDVQLRFLAERLIYLRELDERRTVILQSIREQEKLTPELEQSILAAETKTRL
                     EDLYLPYRPKRRTKAQIAIEAGLEPLAHALWKDPALDPEEQASQFINSEAGIENTQAA
                     LDGARHILMEHFAEDAELINELREYLWQHGIVKSVGNSTKKDQANKFSDYFDYSEAIK
                     KIPSHRALALFRGRREGILQVSLVLPDQDANYGENKVATYFNVTDRQRKADGWLIDTV
                     RMTWKIKLFTKLELELLTRLREIADEEAIKVFSKNLRDLLLAAPAGPQVTIGLDPGIR
                     TGVKVVVVDATGKLLDYSVIFPFAPQNEWHQSIADLAKLAAKYQVNLISIGNGTGSRE
                     TERLVADLIKMYPDLKLTKMIVSEAGASVYSASEIAANEFPDLDVSLRGAVSIARRLQ
                     DPLAELVKIEAKSIGVGQYQHDVNQAKLARCLDGVVEDCVNAVGVDINTASAALLSHI
                     SGLNDTLAKNIVQFRDEHGAFTNRNQLKSIHRMGEKAFQQAAGFLRIMNGDNALDASC
                     VHPEAYPLVEKIISDKKVSIKEIIGNRELLQSINAEQYVDENFGLPTIRDVLRELEKP
                     GRDPRPEFRTAQFKEGVEDIKDLEQGMVLEGVVSNVTNFGAFVDIGVHQDGLVHISAM
                     TNRFITDPRSVVKAGDIVKVKVVEVDKERRRIGLSMKLNDEQAPAVLQKANKPQPARK
                     QAEQKQPEFKKKQEHKKKPDKIKKSEPVKKTVFNTAMADALAKLKRGGE"
     gene            complement(9560..10225)
                     /locus_tag="VAH76_001338"
     CDS             complement(9560..10225)
                     /locus_tag="VAH76_001338"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEO1385973.1"
                     /translation="MNKGQYFWNELWCEGRISFHKEEVNPDLIAYVSSLNTPAKGRIL
                     VPLCGKSVDMLWLVRQGYHVVGIELVEKAILQFVQEHQITVRENTIGQAKQYFTDNLN
                     LWVTDIFALNSALIEPVDAIYDRAALVALPKKLRPAYVDICLKWLKPGGSILLKTLQY
                     NQEKVQGPPYSVSSEEIALSYQQCAKIELLKSQKRIQEPNDHLFNLGISEVNDYVWCI
                     RKG"
     gene            complement(10222..12036)
                     /gene="glmS"
                     /locus_tag="VAH76_001339"
     CDS             complement(10222..12036)
                     /gene="glmS"
                     /locus_tag="VAH76_001339"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO1385974.1"
                     /translation="MCGIMGAVSERDISKILLEGLRRLEYRGYDSAGIAVIDSQDRLK
                     RVRIQGKVQNLADAMQETAIAGNTGIAHTRWATHGKPSEQNAHPHLSHGEIALVHNGI
                     IENHEHLRQQLITYGYQFTSETDTEVAAHLIHYHYQQHENLLIAVQKAAAEMQGAFAL
                     GVIHQKRPEELVAIRKGSPLVLGFGIGENFIASDALALRSFAQSVIYMEEGDSACVTT
                     QDIKVYDSNRILVQRAVHPLNSDSEIVSKGPYRHFMLKEIFEQSKVITDTLESRINSI
                     DVLRASFGEKASHIFPMVKNIHIVACGTSYHAGMIAKYWLESLAGLPTQVEIASEYRY
                     RDVVVPDNTLFITVSQSGETADTLAALFKAKQSNYLASLAICNVATSTLVREADCVFL
                     TRAGIEIGVASTKAFTTQLAAFLMLAAALCKDNRAQEVLRQLQELPACCERVLQMNEE
                     VESLASLFVNKVHALFLGRGVQYPVALEGALKLKEISYIHAEAYPAGELKHGPLALVD
                     KDMPVIAVAPNDELLDKLKSNLHEVSARGGQLFVFVDDSQNWKANGARLIKVPSCGAW
                     LAPIVYTIPLQLLAYHVAVAKGTDVDQPRNLAKSVTVE"
     gene            complement(12400..13701)
                     /locus_tag="VAH76_001340"
     CDS             complement(12400..13701)
                     /locus_tag="VAH76_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO1385975.1"
                     /translation="MIQNNRICRWLFIVLLSLPLVGFTSIPVKSIVVFGDSLSDNGNT
                     THLLKSLRQEEDPAFLVAPFKIFVINKMIEFANDYYVPQMVLDAGISAVTDFFDHDLA
                     PYLANLVGKVKQVPVLPGKPYWKNRFSNGRVWIEYLAEMLSIQKSDEEVYLNKAFGGS
                     WSATYDYQLTVWNLIRHPLGTIKNLIVGKLIPPSLGLIVQAYLLEHEQLSDETLYFIY
                     SGSNDYINVLFFEDNYNTEVMSTYIDNVLDGLSSAVLKLANAGARRFVIMGIPHVGDT
                     PKFVKTTDRDVLNAAVDLHNERLAKRIDAWKERYPTADFLFVNTEQYLKKAFEDPEKY
                     GFYNVKDACIDVKFPMFNAFIHSPFARNYVLQYTQVLQYRDKQFAPGENNYHMCSEPE
                     DYLFWDEIHPTTRAHNYLAYEVCLALEEHGYEVTCKMPESA"
     gene            13954..14718
                     /locus_tag="VAH76_001341"
     CDS             13954..14718
                     /locus_tag="VAH76_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO1385976.1"
                     /translation="MKDIIIASFYKFIPLNDFESLREPILTKMHEIGIKGTIILAHEG
                     VNGGFAGNREQMNVFYDYLRSDSRFADLHFKETYDSKNPFDKAKVKLRKEIVTMGVQK
                     VDPSYNAGTYLSPEEWHQFIQDPNVILLDTRNDYEYELGTFKNAINPDIENFREFPDY
                     VQRNLIDKKDKKIAMFCTGGIRCEKTTAYMKELGFQHVYQLHDGILNYLESIPESESL
                     WEGKCFVFDDRVAVDQKLDRVYPQLPQDYKYEREQK"
     gene            14829..15299
                     /gene="smpB"
                     /locus_tag="VAH76_001342"
     CDS             14829..15299
                     /gene="smpB"
                     /locus_tag="VAH76_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO1385977.1"
                     /translation="MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAG
                     KINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDPVRTRKLLMNKKELNHLIGSVE
                     RQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT"
     gene            15311..15775
                     /locus_tag="VAH76_001343"
     CDS             15311..15775
                     /locus_tag="VAH76_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO1385978.1"
                     /translation="MKIGEMVPDFAFNATNNVQGHLNDYKGQIIVLYFYPKDATPGCT
                     TEGQDFRDAYSQFKDLNTVIFGISRDSLKSHEQFKSKQNFPFELISDDNETLCQTFDV
                     IKMKSMYGKQVRGIERSTFIIDTQGVLTHEWRKVNVKGHVEEVLNAVRALAG"
     gene            15899..17098
                     /locus_tag="VAH76_001344"
     CDS             15899..17098
                     /locus_tag="VAH76_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1385979.1"
                     /translation="MFIRNLQLFIVVASCILVLIGTDILLPSLPNIAHALSVSSNEAK
                     MLISIFMIGQFSTVLIWGVIADRLGRRKTLFLGMIIFFIGSIFSLKANSIQLLLACRF
                     LQGTGAVVVPVAGWALVQDLFPKDEGARIMTWIGTLTAVIPLFAPALGGQLDVLYGWR
                     SNLYCIAFYSFILCVIMLFLPRTIPVQKTTTADLKETLGIYIRIIKNKTFISYIALFG
                     LLNCGEWCFLTVAPFYYSHAHIAADTMGILLMITSMGFVCGSLLASRLFNRLGIDKII
                     ALGIQLAVMSSLMLLIGEYFHWSKYQLYNAITIGVYILSSALLWGATTSRALQCFDDC
                     RGSASAIRSLILLCFAAFGTYSGRLVNHNSLFPVGLFLLFMAFIAFIVFNNKELKTER
                     HMEGIPI"
     gene            17253..18659
                     /locus_tag="VAH76_001345"
     CDS             17253..18659
                     /locus_tag="VAH76_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO1385980.1"
                     /translation="MDNTNTGLKLFVLDTNILMHDPTAIYHFDEHDIYLPMVVLEELD
                     SHKTGTSELARNVRQTNRMLVELMSNATHDQIVSGLPIPSYINSSNKKCSGRLFFQTD
                     EFDQVVPSTLPGHKVDNTILATALGLQKKYYGKRQVIIISKDINLRIKAGILGIHAED
                     YYNDQVLDDVNLLHRGLHILDNNFWDSHAKDMGSWQESGKTFYKVSGPLVSQWNPNDC
                     LSTEDDQFQALVKKIDGPNAIVQLIRDYTQPKHSVWGITARNREQNFSLNLLLDPDID
                     FVSLQGSAGTGKTLLTIAAGLTQVMDQNRYSEILMTRVTIPVGEDIGFLPGTEEEKMT
                     PWMGALMDNLEVLHSSQVGGSFGRGATQDLLQNKIKIRSLNFMRGRTFLNRYIIIDEA
                     QNLTSKQIKTLVTRAGPGSKIICLGDIKQIDTPYLTETTSGLTFAVDRFKSWEHSAHM
                     TLTRGERSRLAFYAAEHL"
     gene            18682..19695
                     /locus_tag="VAH76_001346"
     CDS             18682..19695
                     /locus_tag="VAH76_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HEO1385981.1"
                     /translation="MTDQAITFDDVLLVPSYNHHESRRVVETTSTDRLGKLTLNLPVI
                     SANMDTITESNMANFMHSKGAMGALHRFMTIEENIQEFKKCKGPVFVSVGCTENELQR
                     AEALRDAGADFFCVDVAHAHAKYVGKTLKSLRQLLGSRCIMAGNVATYAGADYLASCG
                     ADIIKAGIGGGSVCSTRIKTGFGVPMLTCIQDCSRADRSIVADGGIKTSGDIVKALAF
                     GADFVMIGGMLAGSAPTPGEVIQKDDGSKVKRYRGMASREAQEAFLGQMHEWKTAEGV
                     ATEVPFRENPDGIIADIVGGLRSGLTYAGADSISELQRKLNYVIVTQAGRLESLPHKL
                     LEG"
     gene            complement(19718..>20009)
                     /locus_tag="VAH76_001347"
     CDS             complement(19718..>20009)
                     /locus_tag="VAH76_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385982.1"
                     /translation="QPTTDSTQPTTGNTQPTTDSTQPTTDSTQPTTDSGSNDTPMQMS
                     PPPESNTSTSTLDLGGQTKYDEVAKTGNSQTPDSGQIPDVQPTTPTMTGGGG"
BASE COUNT         6070 a         4128 c         3645 g         6166 t
ORIGIN      
        1 aggaaattaa catgtcagaa aaaattcgtg gtacagtcaa gtggtttaat gagtccaaag
       61 gtttcggttt tttagaaagt ggcggcaaag attattttgt gcattttagt gcgatccaag
      121 gcagtggttt taaaaccctg gctgaaggcg ctactgtaat gtttaaagct tgcaatggtc
      181 aaaaaggacc tcaagctgag gaagttgaag tagtctaatt tttttatatt agccctgttt
      241 ttttaagtat aatttaatcc ttagaaaagc agggcttttt tttatctgaa acattcttaa
      301 aatttctgtt ctgcatttaa gaaaaactct tctcaagcgg ctactttggc aaatacctgt
      361 gctgataata gctgttatta gcattctgtt tttcaatgga ggcagcatag tagataaata
      421 tcaatccccc atcgtttctg gtagaccgca taaagacgtt cggtcatatt ttttctatta
      481 taaattattt ctgtacaaac cagtatggtt ctcatcaaac tcttgtccaa cttcttcttg
      541 aaccagttct gttgttattc ttaccttatt tgagttattc atgtcagttt gattggtgat
      601 ggttttctca tgattattat ctttttttgc ccacaacttt gctgaatttt ctcgtaatat
      661 ttcagaaggt gacttaactt tcatatcatg aaacacagta gaatatgagt tacttttgct
      721 atcaatgaga tctattttcg aatgctcaga ttttgattgt ttttgttcct tttttttcaa
      781 tcttgaaacg gaatcatatg taaacatact tgataggatg tctcttaaaa atttaccaat
      841 tccttctttt atgaattctc ctatggcact taaatatgaa ccaatttttg agagtatact
      901 ctcaaaaaag taagattctg tttgtcttgt tgatgaagac ttgttccctt gtttttcctt
      961 ctcttgctga cgttctttgc ttgttttttt aatttgagag ggttgaagca atgcatcttg
     1021 atttttcgga ttgatttttt ttagctcaac cattaggttt ttctttttat tgtaagcatc
     1081 acctgctttg tcaaggtacc taaatatatt aagattcatc tcttgttcag cttctgttct
     1141 tagataaaag aaatgagcct cgggcagtcc tttagcacca acttcgggat ggaattgcgt
     1201 ggttataatc ggaacaccaa actctgattc tgctgcttca atggattcat tatcacttgc
     1261 agtacctgta tgttgaaagc ccgctgctaa tttggaaaca acttgctcat gcgcgccaaa
     1321 aaactccgtt tcataatgag gggaattaag agaatgagaa tcttctacat ttaaactcgt
     1381 ttttatattt tgtaattctg tttttaattg atctgactcg cccatttctc ctattaattc
     1441 tcttaaatca aataactctt cttctatatt taatccagtc aactttaata aaagctgcac
     1501 ttcagattta tctaattctt ctaattttcc taatgcatct aatttccctg cttcctgcac
     1561 tatgtttaat ttttccaatg catttatttt ttctgataat ctattatatt ggttcattct
     1621 ttcattaaac gcttttctgg ctgtatccat ttctttctgg acttgctttc caccaataat
     1681 ttgagctatt aatgaactct tatgcagttt gattgcatca taattcatga cttgttgttt
     1741 atttaatttg tcagaattta aatcgcatat ttctccaccg ccatatacag caatgacttg
     1801 atgtccacca caaacaccta gaataggcat acctttttct atcgctacat gcgctaatgc
     1861 caattcagca attgttctgg aaaaatcata attttgatcg gggtctctag tttgattaaa
     1921 aagctccgga tcaatcatgg aattattacc agataatgct aacacatcaa catttttaag
     1981 taattccatt gcttttgctt tggcgtgtgc aaataatctt tgtcgacctt ctttagtgga
     2041 atacaaatta ccaaattctt ctttgggtat gtcttccatc attttcctgt agtcgccgtc
     2101 aataacttcg gaaccgacat atgtaaatga ttctttcaac gaaagaacac tgcttccacc
     2161 aacttcagga ctataggtaa caattattct tgccatgatc ataagcatat ttaaatgagc
     2221 aaatgatatt ataaatgatc attattaagg gatccttatt tattggttgg gttttgagca
     2281 gttcgcaatt tcaggtaatt atttgatgaa actgaaaaat taaaaggagt tctggacaag
     2341 agcaaattat ttgcagccgt gaactatgac ctttgccttg ataccatggg atacaatata
     2401 aagcttgatt agcggcctta ttcttggagc actatttacc ctatttgttt tgcctgcggt
     2461 ttacaacgtc atcaaatcca aactaattga tttgcaaaaa acagggggca aaaaacggtg
     2521 cagcaaagaa taaagattta tttaaaatgc aataagtgga tatggatttt tgcaaatact
     2581 atcagtattc taggcgacat gatttccccg ctaacttatt tatgacttgt aaaggatcta
     2641 cggtattaat ggttccaccg gaaggatttt gcttcaatta tgaaacatca accagcaact
     2701 ctttccaatc attcctttct attaaggata taggtaacaa ggcattagca gaatttactg
     2761 cgatggtgtt acaactggaa aaagaagaga taaaagtatt aaccctctca caaagccggg
     2821 atttacccga tgccgtcttt cccaataact ggttttcaac tcatattgat gcaagaggga
     2881 ataatattct gattatttat ccattattag ctaaaaacag acaagctgaa gtcaatatca
     2941 aaggattaat ggaagtactg aacagagccc aatttgaagt tcatgaaata attgacttaa
     3001 gaaacgataa ggatgaaatc cttgaaggaa ctggcagcct ggttttagat agagaaaacc
     3061 gccttcttta tgcgtcttta tccccgagaa cttccctgaa tttggtaaac aaagtcgctg
     3121 atatcctggg atacacccct attgtattta atagtgtaga taggcaaaac aaacccgttt
     3181 atcataccaa tgtcataatg ggcctaagca agtgttacgc tattatttgc ctggactcaa
     3241 ttaatgatgc tcaacaaaaa gcaatacttt cagaaagttt caaagaaaca ggcaaaacat
     3301 tgattgacat tagttggaat caagtgcagc acatgtgtgg taatgttttg gaactttata
     3361 acaaaaaagg tgagagcctt ttaattttat ccgagcaagc taaaagacac tttactcatg
     3421 agcagctaag aatcatccaa caatactcca gattaatatc gacagatatc cccacaatag
     3481 aagctattgg aggtggaagc gcacgatgca tgatggctga gattttttat taaaagttca
     3541 aaaaattttg ctaccagttt aatttccttt cagatattct ctagagcgag acttaagaaa
     3601 aaccccaagg tcaaggtaaa aaagattgga ggtttactta agtcctgtgg aaaactaatc
     3661 attcaggcag ggaatcatca tgaaaattag tgaattaaaa aaactggttg ctgaattaaa
     3721 taaagaggtt tcaccaaagg ttgaatgcac aaacataatt aacctggctg gcaatttatc
     3781 agaaatcatt gagtattttg agcaggatga acatgctagt ccagagctta tataccgaat
     3841 aaaagcagtt atttgtgagt tctggaagct agttagtctc acattacctt atgaagaatg
     3901 gcaaagcagc attcaagttg ctccctggct tacattacag caatcattat ccaaagccgg
     3961 attactgcct acggatttcc accatcccat tttgtaccag cgcttaaaag aacactatga
     4021 aagctttagt cacagcgaat tgggaattga ccagctcttg cccttgctta tccgttgcag
     4081 ccgcatgact gggtatgcca ataaagatcc agagtctctt gatacttatc ctcactcacc
     4141 attaaacaaa caaattgaag cgagaaggcc acaggaactt gccaagctaa aagacatcct
     4201 ctgcctttta agagccatct tctatttaat tcatcattgt tgcaccattg agcaattgac
     4261 tttaattcct tacctgattt attttcgtaa tcccactaca gatgaagaaa gacgatctga
     4321 attggccatt tttaactggc taatccaaaa accagctgat tgccttgaat tctttaaaac
     4381 caatgaagat tacattgaca caagatcatt tagacaaatt aatgaattag caccattacg
     4441 cccttttatc ccaactgcac gtagtgattt tattaaaata actaataaag aacactggat
     4501 ataccccttt attcaaagca ggacaaatac atcaagatca gagtatgatt tgttgaatga
     4561 tgcagtcaat tggttagaca ctgattttgc aactgagaaa gataaatcct atcatgctgc
     4621 tttggagttt gcacataccg tcaaaaagca agcaaacatt ttaacccaaa gggaaatgaa
     4681 aatagtgcat tccgcgctct atgtcttttg tctggacaaa tacataaaac atcgtaaagc
     4741 agatcctcgt ccaaggtgca ctccgttttc actttcggga gaaacaaaat gcagtgcggc
     4801 cgaaaaaaag cagcaggaga tacttgaaaa accaacgaag tttggatttt tcgaaaatct
     4861 ggccttgaat gaaggaagat taaaaacact gaccaagact tttgaaatgc catcctatac
     4921 tttaccagga aattaatgac atttaattga tgttttgatt ataaaagatg gtgcctgatt
     4981 aaatcaaaat taactcacct caacgcatag gcatggtgga aggagtcctc tcgatcattc
     5041 tgctatttac tcttggctca agtgtgttct tctttcctgc atggtttcta tgctgttgac
     5101 aactgtttga taacatatct ctttcattac tggaatatct ttcaatggat atccttgatg
     5161 aatagcataa gctatcaaaa aactatcctg ccaaaccttt tcatttttcc tgtgttttgc
     5221 cttaacccat aacgctatcc ataaaaaatg aaactgccat ttatcccggt gtaattccat
     5281 ttcctcggaa aataccgcgt ccatagcatc ggccaaacga caaactttaa ctccttccac
     5341 aaagctgctg ttatgattga caattttatc aatggtggga ttttcaatat accatgattc
     5401 tgtaaattgc ttatttttaa gccacatttt cgagcgtttt aaagattctg ctattttttc
     5461 ttgagtaaaa ggaataatct gaacacttaa ttgttcaaat acgcaatcca aatcaagctt
     5521 atcaggtttc aaacggatgc ccagaaattc gagtatttct aaaaaatgca aattaggtat
     5581 atcacttttt tcaacagtaa tggctaaaaa atgctccact attgtttgaa aataagaata
     5641 atcaacttcc cgtaaggtca cactgtcttc gaaagcttta tgataataat ctgtaacttc
     5701 cgctgcagaa atctctggag ttatccaggc atctttaatg cccaaatcat actttaataa
     5761 taagccgcat agcctgtttt ttttattttt ccttgcgtgg ataaaaactc cttgcgaacc
     5821 actgccatcc acttcagttg ctttaatcgt tatttttgct tgactagcct ctgtttcaaa
     5881 tgttactccc ttttttcttt gcgttttaat ccaacgatca aacattccat ggtaatgggt
     5941 tggataccaa tgtttgatta tttgtaatct tgacagtgat gctggcgtca aagtaattgt
     6001 attcattaat tgatcgatag tagacagcgc tatctctcgt acctcgatat taggatggag
     6061 taaaaccagt agtgcaatat ctttaccttc ttcaatacta tacaaatcaa caattaaatc
     6121 tgcaaaaaaa tcaggcggca tggcatagct ttgtgcaaaa aaactctctg ctatgtcaaa
     6181 cagggttaaa tcagataatt catggatcaa atcttttatg gagtccaaat gattaaattg
     6241 ctcatccact tctaccgcat catcatcctg gcttgccaag ccataatatg cttcctttaa
     6301 atcgtcagtt agctcaacat gcacttcata aaatgcattc agtacgggta accaaaagct
     6361 taagctatgc ttatgcgtat caatgacctc agctaaatga ttcattagtt gagacaataa
     6421 ttttgtggca tttttattat ttgcctctgc agctgcttgc aattgagcaa tacaaatgtc
     6481 taaagcgaaa acgcatgctg agtatattgg aggcccatca tgcactaatt cttcactcaa
     6541 attattaata aggtgtatta attcaagact aatttccgga tgttgaaaaa aaaaggcata
     6601 caattgaggg tcaggctctg catcttgctc aatcaataca gccatatcag caattactcg
     6661 ctgcaactct tgagttttat tagtcattgt gtttttggta cctgcctcgg ctttccattt
     6721 tcatcaatcg caacaaaaac aaatatccct tcagtaacct ggtatttttc cattttgatc
     6781 gatgagtaag cccatacctc aacccgaata gtcatagagg tattacccac tttcactaat
     6841 tcaacgtggc agctcactac atcgcctaca tgcacaggtt ttaaaaaagt cattgaatta
     6901 atcgcaacgg tagcgacacg cccatgggaa attctctttg ccaaaacacc tgctgccaaa
     6961 tccatttggg agactatcca tccgccaaat atatcgccat tggcattagt atttaaaggc
     7021 atggccaaag tttggatagt caactctcct ttaggtgttt gcatccctat tctccaccac
     7081 gttttagttt ggctaaagca tcggccatgg ctgtattaaa tacggttttt ttgactggct
     7141 ccgatttttt tatcttatct ggttttttct tatgttcttg cttcttctta aattcaggct
     7201 gcttttgttc cgcttgcttt ctagccggct gtggtttatt agctttctgc aatacagcag
     7261 gagcctgctc atcattcagc ttcatactta aaccaatcct tcggcgttct ttatccactt
     7321 caaccacttt aacttttaca atatcaccgg ctttaacaac agaacgagga tctgtaataa
     7381 agcgattggt cattgctgaa atatgtacca acccatcctg gtgaacgcca atatcaacaa
     7441 aggcaccaaa attagtcaca ttgcttacaa caccttccag aaccattcct tgttccaaat
     7501 ctttaatgtc ctcaacacct tccttaaatt gagcggttct gaattctggt cttggatctc
     7561 gccccggttt ctccaattca cgtaaaacat ctcttattgt tggcaaacca aaattctcat
     7621 ccacatattg ttccgcgtta atactctgca ataattctct attgccgatt atttccttaa
     7681 tactaacttt tttatctgaa ataatttttt ccactaaagg ataggcttca ggatgaacac
     7741 aagaggcatc taaagcatta tctccattca taatgcgcaa aaagccggct gcttgctgaa
     7801 aagccttttc tcccatgcga tggatacttt ttaattgatt tctgtttgtg aaagcaccat
     7861 gttcatcgcg aaattgcaca atatttttag ctaaagtatc attcaatcca gaaatatggg
     7921 atagcaaagc tgcagaggcc gtattaatat ctacgccaac agcattcaca caatcctcga
     7981 caacaccatc caggcaacgc gccaactttg cctggttcac atcatgttga tactggccaa
     8041 cacctattga tttggcttca atttttacca gttctgccaa gggatcttgc aatcttcgtg
     8101 caatagatac agcgcccctt agggaaacat ccaaatcagg aaattcattg gctgcaattt
     8161 cagatgcgga ataaacagat gccccggctt cactgacaat catttttgtt aatttcaaat
     8221 cagggtacat tttaattaaa tcagctacca accgctctgt ttcccgagaa ccggtgccat
     8281 ttcctatgct gattaaatta acctgatatt tcgcagcaag ctttgccaaa tccgcaatgg
     8341 attgatgcca ttcattttga ggagcaaatg gaaaaataac agagtaatcc aacagcttac
     8401 ctgtagcatc cacgacaacc actttaacac ccgttctaat tcccgggtca agacctatcg
     8461 ttacttgagg acctgccgga gcagccagca acagatcacg taaatttttg gaaaacactt
     8521 taatggcttc ctcatctgct atttcgcgca gacgagttaa taactcaagc tccagcttgg
     8581 taaatagctt aattttccaa gtcatcctga ccgtatctat aagccaacca tcagcttttc
     8641 tttgcctgtc agtgacatta aagtaagtcg caaccttatt ctctccataa ttggcatcct
     8701 gatcaggtaa aaccagactt acttgcagaa ttccttcacg ccgcccccga aacaaggcca
     8761 gtgcccgatg agatggaatt tttttaatcg cttccgaata atcaaagtaa tcagaaaatt
     8821 tattcgcttg atctttttta gtactgtttc ctactgattt gactattccg tgttgccata
     8881 aatactctcg taattcatta attaactcgg cgtcttcagc aaaatgctcc attaaaatat
     8941 gccgcgcacc atccagtgcg gcctgggtat tttcgatccc tgcttcgcta tttataaatt
     9001 gggaagcctg ttcttcggga tccaaagcag ggtctttcca taaagcatga gctaatggct
     9061 ctaatcctgc ttcaatagca atttgcgctt ttgtacgacg cttaggccta tagggtaagt
     9121 acaagtcttc caggcgtgtt ttagtttcag cagctaatat gctttgttcc aattccggtg
     9181 ttaatttttc ttgttcacga atggattgca aaatgactgt tcgtctctca tccaattcac
     9241 gcaaataaat tagcctttcc gcaagaaacc gcaattgcac atcgtcaagt cctccagttg
     9301 cttctttgcg ataacgcgca ataaaaggta ctgttgctcc ttcatctaac agccgaatag
     9361 cagtttctac ctgcgatact ttgaccttaa gttcatctgc aataattgcg gctgacgcca
     9421 acatcacttc tgccattcat atccccgtaa aacatgataa aaatcgtctc attatatact
     9481 catcacatac tataatgcga tatagaaatt aagctaatat ataaacaaat tgtccttatg
     9541 cgtaacactg tactgcgttt taccccttcc tgatacacca gacataatca ttcacttcac
     9601 taataccaag attaaataaa tgatcattag gctcctgaat tcgtttttgc gatttcaaca
     9661 actctatttt ggcacattgc tgatacgata aagcgatttc ttctgaggaa acactatatg
     9721 gaggaccctg cactttctct tgattatatt gtaaagtctt tagcagaata cttcccccag
     9781 gcttaagcca cttgaggcaa atatctacat acgctggtcg caattttttt ggcaaagcaa
     9841 ccaaagctgc tctgtcatag atagcatcaa ctggttctat taaagccgag tttaaagcaa
     9901 aaatatcggt aacccataaa ttcaagttat ctgtaaaata ttgttttgct tgacctatcg
     9961 tattctcccg aacagtgatt tggtgttcct gaacaaactg aagaatagcc ttttcaacca
    10021 gttcgatacc aaccacatga tacccttggc gaactaacca aagcatatcg acactttttc
    10081 cacataaagg aaccagaatc cttcccttgg caggtgtatt taaggaagag acataggcta
    10141 tcaaatccgg atttacttct tctttatgaa aagaaatacg tccctcgcac cataactcat
    10201 tccaaaaata ttgacctttg ttcactccac agtcaccgat ttagcaaggt ttctaggttg
    10261 atccacatca gtccctttag ctacagcaac atgataggcc agcaattgca aaggaatagt
    10321 gtaaacaata ggcgcaagcc aagcaccaca ggatggaact ttgattagac gagcgccatt
    10381 cgctttccaa ttttgtgaat catcaacaaa tacaaataac tgcccacctc tggcgctcac
    10441 ttcatgtaaa ttggatttta atttatctaa aagttcatca ttaggggcta ctgcaatcac
    10501 aggcatatcc ttatcaacca aagccagtgg accatgcttt aattcaccgg caggataagc
    10561 ttccgcatgt atataagata tttccttaag ttttaatgcg ccttccaggg ctacgggata
    10621 ttgaacccct cgacctaaaa ataaggcatg aactttattt acaaacaaag aagccaatga
    10681 ttcgacttct tcattcatct gcaaaacacg ctcacaacaa gcaggcagct cttgtaattg
    10741 tcttaaaact tcctgcgcac gattgtcctt acaaagcgct gctgccaaca tcaggaaggc
    10801 tgccaactgt gtagtaaacg ctttggtaga tgctacacca atctcaatac ctgcccgagt
    10861 taaaaaaaca caatccgcct ctctaactaa agtactggtt gcaacattgc atatagctaa
    10921 actagccaaa taattcgatt gctttgcctt aaatagggca gcaagagtat cagctgtttc
    10981 tcctgactga gatacagtga taaataaggt attatcgggg acaacaacat ctcggtatcg
    11041 atattcactt gcaatttcaa cctgtgtagg taagccggca agtgattcaa gccaatattt
    11101 ggcaatcatt ccagcatgat aactggttcc acatgccaca atgtgaatat tttttaccat
    11161 tgggaaaata tgggatgctt tctcaccaaa actcgctctt agcacatcaa tgctattaat
    11221 tcgactttct aacgtgtctg tgattacctt cgattgttca aatatttctt tcagcataaa
    11281 atgcctgtat ggccctttac tcactatctc agaatcactg ttcaacggat gaaccgctct
    11341 ttgaacaagt attcgattgg aatcatagac cttaatatcc tgtgttgtga cacaagcact
    11401 gtcaccctct tccatgtaaa taactgattg tgcgaaagat cttagtgcca aggcatcaga
    11461 tgcaatgaaa ttctcaccaa ttccaaatcc caaaaccaaa ggactccctt tacgaattgc
    11521 aaccaattct tctggtcttt tctgatgaat cactcccaat gcaaaagccc cttgcatttc
    11581 tgcagcagct ttttgtacag caattaataa attctcatgc tgctggtaat gataatgaat
    11641 taaatgcgca gcaacctctg tatcagtttc agatgtgaat tgataaccat aagtaataag
    11701 ttgttgacgc aaatgttcat gattttcaat aatgccattg tgaactaatg cgatttctcc
    11761 atgagataga tgaggatgag cattttgttc agagggttta ccatgcgtcg cccaacgagt
    11821 atgggctata cctgtatttc cagcgatagc ggtctcttgc atagcatccg ccaaattctg
    11881 tacttttccc tgaattctta ctcgttttaa acgatcttga ctatctatga cggcaatacc
    11941 tgcagaatcg tatcctctat attccaatcg gcgcaagcct tccaataaaa tcttgctaat
    12001 atcccgttct gaaacagccc ccataatccc gcacataacc tgctcctctt ttcagcaact
    12061 gtttttgtag aaataagctt ttttcattct tagttcatct actgcatata gaaaagaaca
    12121 aaaggcataa gaatggagct aatttcctaa ataaccggtg ccttaaattt tataaataat
    12181 attccctcat atgaatgaag tatatcaata aaaaagggga attatgacat aaagttttac
    12241 aaatcactaa ggcaaattaa tcttcgtcat catttagaga ctcacggatt gcttaaaaac
    12301 cactctggaa ttacttaaat gaggatcaat tttgacaact tcatcatgaa atcacctgaa
    12361 tcactgttaa tgtgattcag gtgatgatac ctaattgtat taggcggact ctggcatttt
    12421 gcaagttacc tcgtagccat gttcttccaa cgccaaacaa acctcataag caagatagtt
    12481 atgtgctctc gtggttggat gaatttcatc ccagaataaa tagtcttctg gttcactaca
    12541 catgtgatag ttattttcac ctggagcaaa ttgcttatct cgatattgaa gaacctgtgt
    12601 gtattgcaat acgtagttac gagcaaatgg agagtgaatg aatgcattga acatggggaa
    12661 cttcacatcg atacaggcat ccttcacatt ataaaaacca tatttctccg gatcttcaaa
    12721 cgcttttttc agatactgct ctgtattcac aaacaagaag tcagctgttg gataccgctc
    12781 tttccatgcg tcaatccgtt ttgcaaggcg ctcattatga aggtctaccg ctgcatttaa
    12841 cacgtcacgg tcggtagttt tcacaaactt gggagtatcc cccacatgtg gtattcccat
    12901 aataacaaaa cgtctcgctc ccgcattagc cagtttcaga accgcagaac tcaaaccatc
    12961 caacacattg tcaatatagg tgctcattac ttcagtattg taattgtctt caaaaaacag
    13021 aacattaatg taatcattac ttcctgaata aataaaatac agcgtttcat cacttaactg
    13081 ctcgtgttcc agcagataag cttgcactat tagaccaaga ctcggaggaa ttaattttcc
    13141 tactatcagg ttcttgattg ttcccaatgg atgccgtatc aagttccaaa ctgttaattg
    13201 ataatcataa gtggcagacc agctacctcc aaatgcctta tttaggtaga cttcttcatc
    13261 acttttttgt atcgataaca tttctgctaa atactcaatc cagacacgac cgttagaaaa
    13321 gcgatttttc caataaggtt tgccaggcag caccgggacc tgttttactt tacccaccaa
    13381 attggcaaga tagggagcca aatcatgatc aaaaaaatct gtaactgctg atattcctgc
    13441 atcaagtacc atctgtggaa cataataatc gttagcaaac tctatcattt tgttgataac
    13501 aaaaattttg aaaggagcca ctaaaaaggc aggatcctct tcctgacgca ggcttttaag
    13561 taaatgggtg gtattaccgt tatcagacaa gctatctcca aacaccacta ttgatttcac
    13621 tggaatagac gtaaaaccaa ccaaagggag agacaacaat acaatgaaga gccagcgaca
    13681 aatcctgttg ttttggatca taaaaatctc cttatatcaa agctagcgaa acaaaagata
    13741 gcagcttaac ccttagagta tgatcagaat ttaattattg tcaacgattc tttatactta
    13801 agtactaaca tgcaataacg caactatttt tcacccaata tgccgttaaa atcccgatgt
    13861 ccaggaaatg catttaattc attcctattg attaaattgt gagtaaatga taatttttgc
    13921 gaattgattt tttgttaatc cttaggttac agcgtgaaag acattattat tgcatcattt
    13981 tataagttca ttcctctcaa tgattttgaa tcactgagag agcccatact cactaaaatg
    14041 catgaaatag gcataaaagg aactatcatc ctggcccatg agggcgtcaa cggcggattt
    14101 gccgggaata gagaacaaat gaatgttttt tatgattacc tgcgttctga ttcgcgcttt
    14161 gccgacctgc attttaaaga aacttatgac agcaaaaacc cttttgataa agcgaaagtg
    14221 aaactgcgta aagagatagt gaccatgggc gtccagaaag ttgacccctc ttacaatgca
    14281 gggacctatc taagccctga ggaatggcat cagttcattc aagatcctaa tgttatttta
    14341 ttagacaccc gtaatgatta tgaatacgaa ctgggtactt tcaagaatgc aattaatcct
    14401 gacatagaaa atttcaggga attcccggac tatgttcaac gcaatttaat tgataaaaaa
    14461 gacaagaaaa tagccatgtt ttgtactggc ggaattcgtt gcgaaaaaac aacagcctat
    14521 atgaaagagc tgggctttca gcatgtttac caacttcatg atggtattct aaattacttg
    14581 gaatctattc ctgaaagcga atcactttgg gaaggcaaat gttttgtttt tgatgaccgt
    14641 gttgcagtgg atcaaaaatt agatcgtgtt tatccacagt tacctcagga ttataaatac
    14701 gaaagagagc agaaataata tagaatatac tttttgccat aagccgttca aggcaacaaa
    14761 attaagaaaa gcagttcaat ggtgtaatac atcagttata ctgttttttt caacaaatca
    14821 gatgaattat gactaccaaa aaacaaccag attccaccat agccttgaat agaaaagctg
    14881 gttttgatta ctttattgaa gatcaatacg aagcaggcct ggttttggaa ggctgggaag
    14941 taaaaagtct gcgtgctgga aaaatcaatt tgtcggattc acacgtgata atcaaatacg
    15001 gtgaggcatt cctattgggc gcccaaatac aacctcttcc caccgcatcc actcatttta
    15061 ttcctgatcc ggtcaggacg cgcaagctat tgatgaataa aaaagaatta aaccatctca
    15121 taggaagtgt tgaaaggcag ggctatacca tagtccctct ttctttgtat tggaaaaaaa
    15181 ataaaatcaa aataaaaatt gctctggcca aaggaaaaaa agagcatgac aaaagagaca
    15241 cgatcaaaga cagagaatgg caaagagatc gttcaagaat aatgaaaaag aacacttgag
    15301 gagatggttt atgaaaatag gtgagatggt ccccgatttt gcttttaacg ccacgaacaa
    15361 cgtgcaagga catcttaatg attacaaagg acagatcatc gtactttatt tttaccccaa
    15421 agacgcaacc ccgggctgta caactgaagg ccaagacttc agagatgcct actcacaatt
    15481 taaagattta aatacggtga ttttcggtat ttcgagagat agtttaaaat cccatgaaca
    15541 attcaaaagc aaacagaatt tcccgtttga attaatcagt gatgataatg aaacattatg
    15601 ccaaacgttt gatgtgatca aaatgaaatc catgtatggc aaacaagtca gagggataga
    15661 acgaagtact ttcattatag atacacaagg tgtcttgaca cacgaatggc gtaaagtgaa
    15721 tgttaaaggg cacgtagagg aagttctaaa cgcagtgcga gctttggcgg gataaaaaaa
    15781 taatccctga taaggattcc tgttttgaaa aagtactttt tatagagtat ttaattaatt
    15841 tttgctatta ttgttgtgga taaatacatc aaatacaccc ttttcttaag agttcaatat
    15901 gtttatccgc aacctgcaac tttttattgt tgtcgcctct tgcatactgg ttttaattgg
    15961 tacagatatt ttgttaccca gcctgcctaa cattgctcac gcactgtctg tctcatcaaa
    16021 tgaagccaaa atgctcattt ccattttcat gataggacaa ttttctaccg ttcttatttg
    16081 gggcgttatt gccgaccggc taggcagaag aaaaacatta tttctaggga tgattatttt
    16141 ttttattggc tcaatcttca gtttaaaagc caattcaatt cagttgttac tagcctgtcg
    16201 attcttgcaa ggtactggcg cagtcgttgt ccctgttgca ggttgggctt tagtccagga
    16261 tctcttcccc aaagacgaag gagctcgcat catgacctgg attgggacac tgactgcagt
    16321 gattccactc tttgcccccg cattaggggg acaattggat gtgttatatg gttggcgttc
    16381 aaatctttat tgtatcgcat tttactcttt cattttatgc gtcatcatgc tgtttttacc
    16441 cagaaccatc cctgtgcaaa aaacgacgac tgctgacctg aaggaaacac taggcattta
    16501 cattcgaatt ataaaaaaca aaacatttat ttcctatatc gcactatttg gtctattaaa
    16561 ttgtggtgaa tggtgcttcc tgacagtggc tccattttat tattcgcatg cgcatattgc
    16621 tgctgacacc atggggatat tattaatgat tacttccatg ggatttgtct gtggatcttt
    16681 actggcctcc cgcttattta atcgcctggg gattgataaa atcatagcat tgggaattca
    16741 gttggctgta atgagcagcc tgatgttgtt aataggagaa tactttcact ggagcaagta
    16801 ccaactctat aatgccataa ctatcggcgt ctatattcta agttcagcct tgctatgggg
    16861 cgcaaccaca tccagagccc tgcaatgctt cgatgattgc agaggctcgg cttccgcgat
    16921 tcgcagttta atcttactgt gcttcgctgc ttttggtact tattccggtc gcctggttaa
    16981 tcataatagc ctgttccctg ttggattgtt tttacttttt atggccttca ttgcgttcat
    17041 tgttttcaat aataaggaat tgaaaacaga acggcatatg gaaggcatac ccatttaact
    17101 taaaagcaaa cacttaacaa tataattctt gacggaacca ccatagctca gcacaatgaa
    17161 catagaattc agggatttca atgcgctcac tgcccaggca ggaaactgat tccacttgaa
    17221 ttgaagaata ctttagctga cagggtgaac ctatggataa taccaatacc ggacttaagc
    17281 tgttcgtgct tgatactaat attttgatgc atgatcctac tgctatctac cattttgatg
    17341 agcatgatat ttatttgcca atggtggtgc ttgaagaatt ggatagtcat aaaacaggga
    17401 catccgaatt ggcgagaaac gtgaggcaaa cgaaccgcat gttggtagag ctaatgagta
    17461 acgccactca cgatcaaatt gtctcaggac tgcctatccc atcttacatc aatagcagca
    17521 acaagaaatg cagtggacga ttgttttttc aaacagatga atttgatcaa gtggttccat
    17581 caactctgcc gggccataaa gtagacaata caatactggc aaccgcttta ggtctgcaaa
    17641 aaaaatatta tggcaaaaga caagttatca ttatttcaaa agacatcaac ttgcgtatta
    17701 aagcagggat attaggcatt catgctgaag attactacaa tgaccaagtg ctggatgacg
    17761 tcaatttact tcacagagga ttacatattc tcgataacaa tttttgggat agtcatgcca
    17821 aagatatggg ttcctggcaa gaaagtggta aaacctttta taaggtaagc ggtcctttgg
    17881 taagccaatg gaatccaaac gattgcctta gtacggaaga tgaccagttt caagctttgg
    17941 tcaagaaaat tgatggacct aatgccatag ttcaactcat tcgtgactac actcaaccca
    18001 aacattcagt ctggggaatc acggcaagaa accgtgaaca aaatttttcc ctaaacttgt
    18061 tactggatcc cgatattgat tttgtcagct tgcaaggctc agcaggtaca gggaaaacac
    18121 tattaaccat agccgcagga ttaacccagg tcatggatca aaatcgctac agcgagatat
    18181 taatgacaag ggtgacgatc cctgttggcg aggatatagg attcttgccg ggtaccgagg
    18241 aggaaaaaat gactccgtgg atgggcgctt taatggataa tctcgaagtg cttcacagct
    18301 cacaagtcgg cggcagtttt ggacgtgggg caactcagga tttgctgcaa aataaaatta
    18361 aaatacgctc gttaaatttt atgcggggaa gaactttttt aaaccgctat attataattg
    18421 atgaagcgca aaatctgaca tcaaaacaaa ttaaaacctt ggttacaaga gcgggcccag
    18481 gtagtaaaat tatctgtctg ggggacatta agcaaattga taccccctat ttaactgaaa
    18541 ccacttccgg actcactttt gccgtagacc ggtttaaaag ttgggaacac agcgctcata
    18601 tgactttgac tcggggagag cgctcaaggc tggcttttta tgcagctgag catttatagt
    18661 atttgtttat gaggattaat aatgactgat caagcgatca cttttgatga tgttctgctc
    18721 gttccatcgt acaaccatca tgaatccagg cgagttgttg aaacaaccag taccgacagg
    18781 ttaggtaaac tgactttaaa tcttccggta atcagcgcca atatggatac gattaccgaa
    18841 agtaatatgg ctaattttat gcacagtaaa ggagccatgg gcgctttaca tcgctttatg
    18901 accatagaag aaaatattca ggaatttaag aaatgcaaag gacctgtctt tgtatcagtt
    18961 gggtgcactg aaaatgaatt acaaagagca gaagcactgc gggatgcagg agccgatttt
    19021 ttttgtgttg atgtggcaca tgcccacgcc aaatacgtcg gcaaaacatt aaaaagcttg
    19081 cgtcagttat taggaagccg ctgcattatg gctgggaatg tcgccactta tgccggggca
    19141 gattatcttg cgtcttgtgg cgctgatatt attaaagcgg gtattggagg aggctcagta
    19201 tgcagcaccc gaattaaaac cggctttggc gtacccatgt tgacctgtat tcaggattgt
    19261 tcacgtgccg atcgttccat cgttgctgat ggcggcatca aaacatcggg tgatatcgtc
    19321 aaagcattgg cctttggcgc tgattttgtg atgataggcg gcatgttagc cggctctgcc
    19381 cccacgccag gagaggtcat tcaaaaagat gacggaagca aagtcaaacg ctatcgaggc
    19441 atggcatccc gagaagccca ggaagcattc cttggacaaa tgcatgaatg gaaaacagca
    19501 gaaggtgttg cgactgaagt accatttaga gaaaaccctg atggcattat tgctgatata
    19561 gtggggggat tgcgttctgg gcttacctat gccggcgccg actccatcag cgaattgcaa
    19621 agaaaattga actatgtgat tgtcacccag gctgggcgcc tagaaagctt gcctcacaag
    19681 ttgttggaag ggtaaccaac cgactggtca atctatatta ccctccgcca ccagtcatag
    19741 ttggagtagt gggctgaaca tcagggattt ggcctgagtc aggggtttga ctatttccag
    19801 ttttagctac ttcatcatac tttgtttggc caccaagatc tagcgtactt gtacttgtat
    19861 tcgattctgg tggtgggctc atttgcatgg gagtatcgtt actgccgctg tctgtcgttg
    19921 gttgcgtgct gtccgtcgtt ggttgcgtgc tgtccgtcgt tggttgcgtg ttgcccgtcg
    19981 ttggttgcgt gctgtccgtc gttggttgc
//
LOCUS       DATNSN010000045        19947 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.045, whole genome shotgun sequence.
ACCESSION   DATNSN010000045 DATNSN010000000
VERSION     DATNSN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19947
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.045"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            196..852
                     /locus_tag="VAH76_001348"
     CDS             196..852
                     /locus_tag="VAH76_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEO1385983.1"
                     /translation="MQNIQHLIDYILHIDVYLNAFVSTYGFWTYLALFAVIFCETGLI
                     VTPFLPGDSLLFAAGSIAAQPGNPMNIMLLFVLLFMASILGNQVNFLVGRAIGPRVFS
                     AKRSWLLNPKHLHDTHAFYEKHGGKTIILARFIPIIRTFAPFIAGIGRMRSLHFTLFN
                     LISAILWIGSLLSLGYYLGSIPLIKENFSLVIYGVIAISILPPIIALINKKSTSSIKK
                     "
     gene            complement(916..3150)
                     /gene="lem21"
                     /locus_tag="VAH76_001349"
     CDS             complement(916..3150)
                     /gene="lem21"
                     /locus_tag="VAH76_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem21"
                     /protein_id="HEO1385984.1"
                     /translation="MAKTIKATGDGACLFNAVSIGLSVEILSGRLDSQLDTPGYQALL
                     DEFAKHHPQFNPKSWKTLKEWLAYYNDTRDIELILAPVLFNLNQKYQDHLDEEILNEL
                     TNLVWKNKANIENGQAWFQLQNTGDLGEALFPKLENLDLKKDRTPLLDKLREILKDYK
                     LELTRENVKQFLTEKAKELLSALKKKISSDPHAFQRGYSCDELKGMTDALAISLVENR
                     EEDITDNRIKIRLENQEEHWNVLCNEEDSERFLDSTPSRLKMTSLEAYRGDKQVSAPT
                     SEQLDLIEEPGVFLRERTIDNPGLGNCAFYAFAIGLVNIIQEEAKYNRRTMFDRWVGL
                     DRSISGQYDEILKLNLEDPDKELLDRLQSSLRIVTYQYQIRELRNVCVFRNGNYNRLT
                     GNSNFVNFAALYYGDPLDTDSRFNPFADSVPILIKMANTDRDSVHPGHENDVLVPLFL
                     DLLYGDATNPADITLETEPKSDSPIITAMNNITQDFFWGTHLDLNYLAEAFEVNLHVL
                     RNNSPIQEFVDIPERHTLTLTNSNNTHWTTQITTARATTGMLLGRNPHLSGAKRTGEQ
                     QRVNEEFPGKESANDFMIPPFDEISNPHHKKEVETNKPMGKGFFHDRPVVSEEQRKLE
                     QLKRIVANATLAYTTYSESIWFCFFHYHGQTGRDRANNFCKTFSKITDYSQAKEALVH
                     YLNDSRNGNTHPHSYRTMLLHELQGNHDNKKDLQYLSRHFTSSLNKLASVLEVQLFAN
                     RYVP"
     gene            complement(3241..3939)
                     /locus_tag="VAH76_001350"
     CDS             complement(3241..3939)
                     /locus_tag="VAH76_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEO1385985.1"
                     /translation="MNLGILLCDKVSEVFVADHGQYPEMFANLLRPADSTLEFTVFDA
                     EHGELPTDVHAVDAYLISGSRHGVNDNYPWIRKLEEFVCTLHASQKKLIGICFGHQLI
                     AKALGGKVIKSPKGWGVGMSQNQIYQLKEWMRPSLNCFNLLVSHQDQVIELPTGAEIL
                     AGSDFCPNYMMQIGSFFSVQGHPEFTKSYSRDLMVSREDSVNEIEFAKGMKSLELHED
                     DTIIAQWIVNFLKA"
     gene            complement(4069..5229)
                     /locus_tag="VAH76_001351"
     CDS             complement(4069..5229)
                     /locus_tag="VAH76_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEO1385986.1"
                     /translation="MKNQSLIVNWNYPTRILVGAGRINELAKACRELGMNAPLLVTDP
                     GLASSLMVSKVIQQAQASGLRTGLFCQIKTNPTGDNVMNGVCAFKAGQHDGVIAFGGG
                     SALDAGKAIALMVGQDRPLWDFEDVGDNWTRVNVSAMAPVLAIPTTAGTGSEVGRASV
                     ITDAEKQVKKIIFHPKMLPAMVILDPELTVGLPKLLTAATGMDALSHCLEAYCSNYYH
                     PMAEGIALEGIRLIKENLIQAYKDGSDLEARTHMLVASAMGATAFQRGLGAMHALAHP
                     LGAIYDIHHGRLNAVLMPYVLLANRSEIENKIERLANYLSIANGFDGFLDWIEQMRLE
                     LGIENTLSQLGIDHSQIDRLAKMATEDAAAASNPVLFTFEQYKELLSKAIGE"
     gene            complement(5359..6756)
                     /locus_tag="VAH76_001352"
     CDS             complement(5359..6756)
                     /locus_tag="VAH76_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEO1385987.1"
                     /translation="MIATLKTYSPVDNSLYVEREYANQEEIKTALSKAQVAKKLWSLS
                     SLAERQEYCLAAVNEIVACKVDIAEEICWQMGRPIRYAAGEINGFEERARYMIAQAES
                     SLASVELPEKEGFTRYIKREPLGLVLVIAPWNYPYLTAVNAIIPALMAGNVVILKHSA
                     QTPLVAERFDQAFKKAGLPMGVFQYLHLTHEDTEQLMRSPAVNSVAFTGSVAGGERVE
                     QTLAGRFIPVNLELGGKDPAYIRADADLEHAVETVIDGAFFNSGQSCCGIERIYVHKE
                     IYEQFVNKAVNLVKQYKLGRPDDPETTLGPLVRASSADFVREQIKEAIAQGAIAHINT
                     QDFVMDRPGTPYLAPQILTDVNHRMRIMTEETFGPVVGIMSVDSDEEAIERMNDSDYG
                     LTAAVFTQDIHRGIAIGERLQTGTFFINRCDYLDPGLAWTGVKKSGRGCTLSAIGYEY
                     LTRPKSFHIRTKIET"
     gene            complement(6794..8167)
                     /locus_tag="VAH76_001353"
     CDS             complement(6794..8167)
                     /locus_tag="VAH76_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEO1385988.1"
                     /translation="MLNPREVRTIEDAKRIVEERQLTHVKVGLFDIDGVMLGKYMSRN
                     KFFSALEHGFAFCDVILGWDSKDKLYDNVKYTGWHTGYPDASVRIVPSTCREILFEEN
                     QLLFMAEFSQAAEAICPRGILRRVINKAAGMGYDVYAALEYEFFVFDETPDSVRAKGF
                     RNLKPVTPDNFGYSIIRNTVHAEFYHQILTMAEKMDFPIEGLHTETGPGVLEAAIAVD
                     DAEAAGDKAALFKTFMKILAQRNNKMATFMAKWSGDYPGQSGHIHLSLRDRDGGKSAF
                     YESGREYNMSKIQRHFLAGQQKLMPEFLAMLSPTVNSYSRLIPGYWAPTEATWGVENR
                     TTALRVIPGSDKSQRIEYRLGSADANPYLALAAAIGSGLYGIENELEPEAEVKGNSYE
                     QDHSPELALPRTLWESAQNLKNSSAAYELFGQEFVEHFAASREWEEREFRKHITDWEL
                     DRYFEII"
     gene            complement(8219..9007)
                     /locus_tag="VAH76_001354"
     CDS             complement(8219..9007)
                     /locus_tag="VAH76_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEO1385989.1"
                     /translation="MKSLLTLQTQFQEFIYSGQGSIKESIVPTKLVSTETRLNIYKDG
                     YRLRLIECLAANYPALYAYLGTEEFEKLSARYIDSYPSAYRSIRWYGDRLPELIKKYY
                     AKSCYYLYELAELEWKMTLAFDAADDTVLNVEEMAAVPPESWAEMGFVLHSSVHRLDF
                     CWNVFSLWQALVNNLEIPELKDSPAATGWVLWRGQNYIIQYYSLSEEEAWALDALIQG
                     SSFGAICEGLCQWMPAEEVGMKAASYLKNWIQNGLLSQCRIRRN"
     gene            complement(9004..9867)
                     /locus_tag="VAH76_001355"
     CDS             complement(9004..9867)
                     /locus_tag="VAH76_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEO1385990.1"
                     /translation="MEVSPNKSDQKLPYLGFGLGLRPNYYEEILTSKPDLDWFEILTE
                     NYLIPGGKPLYYLDKIREHYSIVMHGVSLSLGSTDPLNWDYLKQVQELASRIEPVWIS
                     DHLCWTGVHGLNTHDLLPVPYTTEAIQHIVSRIQEIQDFLKRPFLIENVSSYLTYKQS
                     EMSEWDFILEIVKQSGCYLLLDVNNVYVSSFNHNFDPMAYINSMPPGRVAQIHLAGHT
                     SHGDYIIDTHNAPVIEPVWDLYEATIQRLGPVSTMIERDDNMPDFSELLSEINHAKRL
                     SIQAAKEKVAV"
     gene            complement(9943..10194)
                     /locus_tag="VAH76_001356"
     CDS             complement(9943..10194)
                     /locus_tag="VAH76_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385991.1"
                     /translation="MNKLQLTGAALAVGAASMFALAPAFAVDATTTTIHCKGVNACKG
                     QGSCKTADNACKGQNSCKGKGILEMSKEDCEKQGGTVAE"
     gene            complement(10624..11982)
                     /locus_tag="VAH76_001357"
     CDS             complement(10624..11982)
                     /locus_tag="VAH76_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1385992.1"
                     /translation="MKLSFLGATSTVTGSKYLVESNNKKILVDCGLFQGLKELRLRNW
                     SPLPVSPESIDAVVLTHAHIDHSGYLPLLVKQGFKGRIYATAATTDLCSILLPDSGFL
                     HEEDARRANKYGYSKHHPALPLYTQEDANNTLKYFESVEYDTPYPLMEDCEISWHRAG
                     HILGSAFIQFQTKQIKLLFSGDIGRLNDPVMKPSELMKSTDYLVIESTYGNRLHDKTD
                     PLAFIANIVNTTCKRGGTVLIPAFAVGRAQTMLYYLYILKQKKLIPDVPVYLDSPMAI
                     DATELLLKHHNEHRLSAEECRAVCQIATYVHTPNESMAVDWGRESKIIISASGMMTGG
                     RVLHHLKVYAPDPASTILLTGFQAAGTRGEKMLNHEPSIKIHGDDYPVRAQVAMLTNA
                     SAHADYEEILVWLKHFEKAPKEVFITHGEPQAALGLKGHIEAELGWNCKIPNYLEKVN
                     LD"
     gene            complement(12105..12755)
                     /locus_tag="VAH76_001358"
     CDS             complement(12105..12755)
                     /locus_tag="VAH76_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1385993.1"
                     /translation="MNYFIGLCFGLLNAVICYAEPNGSSLPKELIKVNKENNPKCVEF
                     VTYKGELYCSVVPLADSSGDSQVIYYEKQIVRFDDRPWKIAWGKKTDKVVTIEYIPVG
                     DDINNWKELITTQFMPGLINMTPAHFGDQFLYNLNKSGVKYIVHVIEEQPNLLIFEFK
                     IQEPANLQQDEILKITKGKDGIYVLHYAIKKSDMTKENRDKWVKNLKSSFIKASTP"
     gene            12872..13162
                     /locus_tag="VAH76_001359"
     CDS             12872..13162
                     /locus_tag="VAH76_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HEO1385994.1"
                     /translation="MNGCKISPVGLGLSLGILWGLSLLVMGLIAYFYAYGRPFVEAVA
                     TLYLGYEPSVLGSFIGGVIGFIDGFVTGFLIAWLYNCFSCCSCCRGKENKKE"
     gene            complement(13253..13564)
                     /locus_tag="VAH76_001360"
     CDS             complement(13253..13564)
                     /locus_tag="VAH76_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEO1385995.1"
                     /translation="MLKCLSSVIIVFLILFTAGCQTNTVSNMFIPSNPSGMTLAQSVK
                     DSLMESNDPVINQIHVESNQNVVILSGYVKKIRQSDIAEQIARQVQGVQSVENRIIVR
                     P"
     gene            13781..15568
                     /locus_tag="VAH76_001361"
     CDS             13781..15568
                     /locus_tag="VAH76_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1385996.1"
                     /translation="MKKTTEKPINKTKEMPASSKKEILNPLEQTPSPEQSDPIFRFID
                     KLYQANLGKLTAGISPAALGTAYYSWFAQLLQSPGSMLRLAYYPLLHANDYLSNLFKY
                     DKPRDGKDVRFHTENWSYYPWRLWAEQFLQFEDWCLQASGKVPGIPLHVKRTVTFSTR
                     QILDALSPSNFVLTNPDLLQETIRSNGQNLIRGTELAFQDFVEKITGSPPAGVENFIP
                     GKQVAVTKGKVVYSNHLIELIQYTPQTEKVYKEPILILPAWIMKYYILDLLPENSLVN
                     WLVRQGHTVFIVSWRNPTKEDRNLGLDDYYKLGAMDAINAVSNAIPHTKIHLMGYCLG
                     GTLALLTAAAMAHDHDNRLKTLSLLAAQGDFIDAGELLLFITKSEVSFLKSMMWEQGY
                     LDTKQMSGTFQMLRSYDLIWSKMVQDYMHGTQRGMIPLLAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRFILDGKPVVGENIRIPAFVVSTEKDHVAPWKSVYKTHLLINSDITF
                     VLTNGGHNAGIVSEPGHEGRYYRIRERKMDSTYLDPTNWLKKAELREGSWWIAWHDWL
                     VNHSSQKQVSAPKLDKKLPNAPGKYVLQK"
     gene            15580..16986
                     /locus_tag="VAH76_001362"
     CDS             15580..16986
                     /locus_tag="VAH76_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEO1385997.1"
                     /translation="MNNEFLENVTYEELTIGRKASLNRTLTQDDINLFATMSGDINPS
                     HVDPQFAKSDVFHGVVGHGMWSGALISTLLGTALPGPGTIYLEQDIQFITPVRIGDNL
                     TITIVVRDKHPGKSIVHFDCMGVNQKGETVIKGLAKVIAPTKKMKVPRTVLPLVQIHH
                     NDHFNKIIEACSNLPSIKTAVVHPVTANVLEAVYDSVKAGLITPVLIGPMAKIKCAAS
                     EAKIDLSNWEMIDTEHSDAAAFRAVELAASGKVDAIMKGALHTDELMSAVVPATSGLR
                     TKYRISHAYVMDVPTYHKPLIITDAAINIAPNAADKADICQNAINLWRILYGEEIKPK
                     VAILAAVEMVNPKMQATVDAAILCKMADRAQIVDGILDGPLAFDNAINKQASKEKNII
                     SPVAGDADILLVPDIESGNMLAKQLTFLGHADAAGIVLGARVPIILASRADSLRTRLL
                     SCAVATKIAAARKAGKIK"
     gene            16983..18101
                     /locus_tag="VAH76_001363"
     CDS             16983..18101
                     /locus_tag="VAH76_001363"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEO1385998.1"
                     /translation="MRHSALLIINSGSSSVKFQVFSNTEKLVLLAKGKIINIGGEPSF
                     TAVNKTSEHQNTLITLEKDMTHEQAIHLILDWISQQQQNWEIHAVTHRVVHGGEYFTQ
                     SVLVTPQIISQLETLNSLAPLHQPHNLAAIKIIKKIKPELPQIACFDTAFHAQHTPLF
                     TTCALPEYLRQQGIRRYGFHGLSYEWISLVLKKDYPDLANNRIIIAHLGNGASLCAMD
                     KGVSIDTTMSLTALDGLPMGTRCGSLDPGAVIYMMRELNLPPDKVEEILYNESGLLGL
                     SGKTNNVLELQSSPDKNAQFALEYFCLKTAQFIGMMTISLGGVDGIVFTGGIGENSEP
                     IRANILKRIECLHPFSAIVIPANEEKMMAMHTLTALRT"
     gene            18141..19031
                     /locus_tag="VAH76_001364"
     CDS             18141..19031
                     /locus_tag="VAH76_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEO1385999.1"
                     /translation="MDKDYYKIMGVSQDASEKDIKMAYRKLARKYHPDISKEPDAEER
                     FKEMAEAYEVLKDPKKRSEYDQYLKYKEFNPQNDGFTGRRTQEQPFQEFHFDSDFFET
                     LFGHSRYQQQPMTGQNYHGKITISLEEAYHGAVKNLQVPVEQGTETKMQTLKVKIPAG
                     VKSGQQIRLAGQGGSGSGGGARGDLYLTVEVIKHPIFDVMENDIYLTLPITPWEAALG
                     ATVVIPTLAGKIDLKIPPGSQGGQKLRIKNRGLPGSPPGNQYVLLKIITPPAQTEEAK
                     ALYKKMAEVMPYNPRKTMGV"
     gene            19034..19360
                     /locus_tag="VAH76_001365"
     CDS             19034..19360
                     /locus_tag="VAH76_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEO1386000.1"
                     /translation="MSKDNLKDNLLIGMLIEETTTISFIEVCQKYHIPEELFIEMIEQ
                     GLFPDQPSDPEKIALGQKELRRLESAFRLHRDLGINLPGVALALDLLEKIDQMHHELE
                     ILRKHF"
     gene            complement(19357..19920)
                     /locus_tag="VAH76_001366"
     CDS             complement(19357..19920)
                     /locus_tag="VAH76_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEO1386001.1"
                     /translation="MQKICRFIFKLIGWKIVGELPNEKKYIVIVAPHTSNWDFVICLC
                     ARFVVGVKINFLAKHQLFFFPLSYFLRAVGGTPVDRSKKGNRVEKVVELFRQRDELKL
                     GLAPEGTRSPVTRWKEGFYHIARQAGIPIVMVGPDYSTKEIRIHEPFWTTGDIKKDFQ
                     YIIEFFRTIKGYRPKEIPDYNPKDSDA"
BASE COUNT         6048 a         4309 c         3651 g         5939 t
ORIGIN      
        1 cgttcaggct gaggagatgc ataagcatcg tctcgaagtc ttggtatgaa acattaaatc
       61 tcgtacaagg cttcgagacg gcatataaat gcctcctcag cccgagcggt ttgagataac
      121 aactgatttt atccataact cacgttagta tagatacatg gaattcacgt catacattta
      181 atccaaagag acactatgca aaacatacag cacctgattg actacattct tcatatcgat
      241 gtttatttaa atgcctttgt atcaacatat gggttttgga cttatcttgc gttatttgct
      301 gtcatctttt gtgagacagg ccttatcgtc actcctttct taccaggaga ctccctgtta
      361 tttgctgcag gaagtatagc agctcaacca ggaaacccta tgaatatcat gctgttgttt
      421 gttttgcttt tcatggcttc gatattaggg aatcaagtga atttccttgt tggacgtgca
      481 ataggccctc gtgtctttag cgccaagcgc tcctggttac tgaatcccaa gcatctgcat
      541 gatacccatg ctttttatga gaagcatggc ggcaaaacaa taatactggc gcgttttatt
      601 cccataatac gcacctttgc tccatttatt gcagggattg gcaggatgcg atcattgcat
      661 ttcacgcttt tcaatctgat cagcgccata ctttggattg gaagtcttct tagcctggga
      721 tattatttag gctcaatccc gttaataaaa gagaatttct cattagttat ctatggggtc
      781 atagcgattt caattttacc gcccatcatc gctttgatta ataaaaaatc gacgtcctca
      841 attaaaaaat aatatactct aggaactcat gcgggtgcaa agggctctaa ggatgccttt
      901 tgcaccacgc tcttactagg gaacatatct gttggcaaac aattgcactt ctaaaacaga
      961 tgctagttta tttaaagaag aagtaaaatg tctggacaaa tattgcaaat cttttttatt
     1021 gtcatgattc ccttgtaatt catgcagtaa cattgttcta taggagtgtg gatgtgtatt
     1081 tccattcctg ctgtcattca aataatgaac caaagcttct ttggcttggc tataatctgt
     1141 tattttagaa aatgttttgc agaagttatt agcccgatct cggcctgtct gaccatgata
     1201 atgaaagaag caaaaccaga tgctttcact gtaggtggtg taagcgagcg tagcattagc
     1261 aacaattcgt tttaattgct ccaattttct ctgctcttca ctaactaccg gtctgtcatg
     1321 gaaaaaccct ttacccattg gtttattggt ctctacctct ttcttatgat gggggttaga
     1381 tatttcatca aaaggaggta tcataaaatc gttggctgat tcttttccag gaaactcctc
     1441 gttaactcgc tgttgctccc cggttctttt agctccactc aggtgagggt ttcgccccaa
     1501 aagcattcct gtagtggctc tcgccgttgt aatttgggta gtccaatgcg tattattgct
     1561 attagttaaa gtaagagtgt gtcgctcggg aatatcgaca aactcttgaa ttggactatt
     1621 attccttagc acatgaagat tcacctcaaa cgcttcggct aaataattta aatccaaatg
     1681 ggtgccccag aaaaaatcct gagtgatgtt attcatcgca gtaatgatag gtgaatcact
     1741 ctttggttct gtttccaggg tgatatcagc tggattcgta gcgtcaccat ataataaatc
     1801 taaaaagaga ggaaccaata catcgttttc atgtcctgga tgaacactgt ctcgatcagt
     1861 gtttgccatt ttaatcaata taggaacaga atcggcaaaa ggattgaacc gagaatctgt
     1921 atccagagga tcgccataat ataaggctgc aaaattaaca aaattgctgt ttccagttaa
     1981 tcgattataa tttccatttc tgaatacaca gacatttctt aattctcgta tttgatattg
     2041 gtaggtcact attcttaagg agctttgcaa cctgtccagt aattctttat cgggatcttc
     2101 aagattcagt ttcaaaatct catcgtattg acctgaaatt gacctgtcga gacctaccca
     2161 tcgatcaaac atggttcttc tattatactt tgcctcttcc tgaattatat tcaccaaacc
     2221 tatcgcaaag gcataaaaag cgcaattgcc aaggccaggg ttatcaatgg ttctttctct
     2281 taaaaaaacg ccaggctctt caatcaaatc caattgctca gatgttggtg cactaacttg
     2341 tttatcacct cgataagcct ccaaggaagt cattttcaaa cgtgagggtg tcgaatccag
     2401 aaaacgctcg ctatcctctt cattacacaa tacattccaa tgctcttcct ggttctctaa
     2461 tctgattttg attcggttgt ccgtaatgtc ctcttctcta ttctcaacca gactgatcgc
     2521 taaggcatct gtcataccct ttaactcatc acagctataa cccctttgaa aggcatgagg
     2581 atctgaactg atctttttct ttaaagcgga caataattct ttagcctttt cggtcagaaa
     2641 ttgttttaca ttttcacgtg tcagttcaag tttgtagtct ttaagaattt ctcgtaattt
     2701 atctaataaa ggagtcctgt cttttttcaa atccagattt tccaatttgg gaaaaagtgc
     2761 ctcgcccaaa tcccctgtgt tttgcaattg aaaccaggct tggccatttt caatattggc
     2821 cttatttttc catacaagat tggttaattc attgagaatt tcttcatcca gatgatcttg
     2881 atatttctga ttcaaattaa ataatacggg tgccaaaatc agctcaatgt ctctggtatc
     2941 gttgtaatag gctagccact cttttaaagt tttccagctt ttggggttaa attgcgggtg
     3001 atgtttggca aattcatcca gcaaggcctg ataaccgggt gtatccagct gcgaatccag
     3061 tcttccgctc aagatctcga cactcagtcc gattgacact gcattaaata gacaagcgcc
     3121 atctccagta gccttaattg tcttcgccat acatactccc gatagttctg gtcagtgaat
     3181 aaaattaatg ttcacatatt gtactctaag ggggtataat aatcaattaa aaacagctca
     3241 tcaggctttt aaaaaattaa ctatccactg ggcaataatt gtgtcatcct catgtaactc
     3301 aagagatttc atcccttttg caaattcaat ttcattgacc gaatcctcgc gagacaccat
     3361 caaatcacga gaataagatt tagtaaactc aggatgcccc tgcacactaa agaaagaacc
     3421 aatttgcatc atataatttg gacaaaaatc actccctgcc aatatctccg caccggttgg
     3481 taactcaatc acctgatctt gatggctaac tagcaagttg aagcaattta gagatggtct
     3541 catccattct tttaattggt aaatttgatt ttgtgacata cctacccccc aacccttggg
     3601 agatttaatc acttttccac ctaaggcttt cgctattaat tgatgcccaa agcaaatacc
     3661 aattaatttt ttttgggaag catgcaaagt acaaacaaac tcttcaagct ttcttatcca
     3721 gggataatta tcattcaccc catgtcgact tcctgatatc agataagcgt ctacggcatg
     3781 gacatctgtt ggcagctccc catgctctgc atcaaataca gtaaattcaa gagtagaatc
     3841 cgccgggcgc aatagattgg caaacatctc agggtattga ccatgatccg caacaaatac
     3901 ttcacttacc ttatcacaaa gcaaaatacc aagattcatt taattcacct aattaattat
     3961 cgcaaaattt ttattgtctg gattagactt aatttcaata tacaagtcct ttccatctaa
     4021 tttatacctg gcaaacaacc cgtttctgca tcttttcctt tacaacgatt attcaccaat
     4081 agctttgctc aatagttctt tgtattgctc gaatgtaaaa agaacaggat tggaagctgc
     4141 tgccgcatcc tcagtggcca tttttgccag tctgtctatc tgcgaatgat caatacctaa
     4201 ctgacttagt gtattttcga ttcctaactc taatctcatc tgctctatcc aatccaaaaa
     4261 accatcaaat ccatttgcta ttgacaaata atttgccaga cgctctatct tgttttcaat
     4321 ttcactacga ttagccaata aaacataggg catcaaaacc gcattaagcc gcccgtgatg
     4381 gatatcataa attgcaccta atggatgagc caaggcatgc atcgctccaa gaccacgctg
     4441 aaaggcagta gctcccattg ctgaagcaac tagcatatgg gttcttgcct ccaggtcact
     4501 accatccttg taagcttgaa ttaaattctc cttgattaat cttattcctt ctaacgcaat
     4561 accttctgcc ataggatgat aataattgga acaataagct tcaagacaat gggaaagagc
     4621 atccatacct gtggctgcag tgagtaattt gggaagcccg actgttaact ctggatccag
     4681 aataaccatg gcaggcaaca ttttaggatg aaaaataatt tttttaacct gcttttcagc
     4741 atcggtaatt acagaagctc gcccgacctc tgaaccagtt cctgctgttg ttggaatggc
     4801 aagaactggg gccatggctg aaacattcac tcgagtccag ttatcaccca catcctcgaa
     4861 atcccataaa ggtcgatcct ggccaaccat taaagcgatg gctttgccgg catccaaagc
     4921 cgagccacct ccgaaagcaa tcacgccatc atgctggcca gctttaaaag cacagactcc
     4981 attcatcaca ttgtcgccgg taggatttgt tttaatttgg caaaataacc ccgttcgcaa
     5041 accggatgcc tgagcctgtt gtatgacttt actcaccatt aacgacgaag ccaatcccgg
     5101 gtctgtgact aacagaggag cattcatgcc taactcacga catgctttcg ctagttcatt
     5161 gattcgccca gccccaacca ggattcgtgt cgggtaattc caattgacaa ttaatgattg
     5221 gtttttcatg tatgatcttc ctttgattcg aaaaagacca ttgtaactga tagcaagtac
     5281 ctttccgcag gaaagattag gtctttttta taattttatt ttgagtttta agacgtaagt
     5341 aatcgtaggt atcaggcttt aagtctcaat tttagttctg atatggaatg atttgggccg
     5401 cgttaaatac tcatagccta tggcggataa agtgcaaccc ctgcctgatt tttttacacc
     5461 tgtccaagcc aaaccgggat caaggtagtc gcaacgattg ataaaaaaag tccctgtttg
     5521 taatcgttca ccaatcgcta ttcctcgatg aatatcctga gtaaatacag cagcggtcaa
     5581 accatagtca ctatcattca tcctttcgat tgcctcttca tcactatcaa ccgacatgat
     5641 ccctacgact gggccaaaag tttcttctgt cataattcgc atgcgatgat taacatcggt
     5701 taaaatttgc ggtgccagat agggtgtacc aggtctgtcc attacaaaat cctgggtatt
     5761 tatatgtgct attgcccctt gggctatcgc ttctttgatt tgctctctga caaagtcagc
     5821 tgaggaagca cgtaccagtg gacccagagt ggtttctgga tcatcgggcc gtcccaattt
     5881 atactgttta accaaattaa ctgctttatt aacaaattgt tcgtatatct ccttatgaac
     5941 ataaatcctt tctataccac aacaggattg acctgaatta aaaaacgcac catctatgac
     6001 tgtttctact gcgtgctcca aatcagcgtc tgctctgata taagccggat cttttccacc
     6061 tagctccaaa ttcacaggaa tgaaacgtcc tgccaatgtc tgttcaactc tttcaccacc
     6121 agcaactgaa cctgtgaaag ctacggaatt aactgctggc gatctcatga gttgttctgt
     6181 atcttcatgt gtcaaatgca aatattgaaa aacacccatg ggtaagcctg cttttttgaa
     6241 tgcctgatcg aaacgctcag ccactaacgg agtttgcgca gaatgcttca aaatcacaac
     6301 attacccgcc ataagtgcag gaataatcgc attgactgct gtgagatagg ggtaattcca
     6361 tggcgctata accaacacaa gccctaacgg ctctcgtttg atgtatcttg taaatccttc
     6421 tttttcaggt aattccacag aagccagtga tgattcggcc tgtgcaatca tatatcttgc
     6481 tctctcttca aacccattga tttcaccagc tgcgtatcga attggtctac ccatttgcca
     6541 acaaatttct tctgcaatgt ctactttgca agcgactatt tcattgacag cagccaaaca
     6601 atattcctgg cgttcggcca gagaagacaa agaccacagt ttttttgcta cttgtgcttt
     6661 gcttaaagca gtttttatct cttcttgatt cgcatactct ctctcaacat aaagagagtt
     6721 atcgacagga gaataggttt ttaaggtagc aatcattttt aatccataag ttttataaaa
     6781 tctgtcgttt caattaaata atttcaaaat acctgtcgag ttcccaatca gtaatatgtt
     6841 ttctaaattc tctctcttcc cattctcttg aagcagcaaa atgctcaaca aattcttgac
     6901 caaataattc ataagcagca gaagagtttt ttaaattttg tgccgattcc cataatgtgc
     6961 gaggcaacgc caattccgga ctatgatctt gctcgtagga attccctttt acctcagcct
     7021 caggctccaa ctcattctca atgccatata aaccggaccc tatggcagca gccaacgcga
     7081 gatatgggtt tgcatctgca gaacccagcc gatattcaat acgttgtgac ttatcactgc
     7141 cgggaatcac tcgtaaagca gtggtgcggt tctcgacacc ccatgttgcc tctgttggag
     7201 cccaataacc gggaattagt ctggaatagc tattcacagt cggggataac atggctaaaa
     7261 attcaggcat caatttttgc tgcccggcta aaaaatggcg ttgaattttg ctcatattat
     7321 attctctgcc tgattcataa aaagcagact tgccgccatc tctatctcgc aaagacagat
     7381 gtatatgccc actttgccca ggataatcac ctgaccattt agccataaaa gttgccattt
     7441 tattattgcg ttgcgcgaga attttcataa atgttttaaa caaagccgct ttatctcctg
     7501 ctgcctcggc atcatcaaca gctatcgctg cttccagaac tcccgggcct gtttcagtat
     7561 gtaaaccttc aatggggaaa tccatttttt ccgccatagt gagaatttga tgataaaatt
     7621 cagcatgtac cgtattacga ataattgagt agccaaaatt atcaggagta acaggcttta
     7681 gattacgaaa tccttttgca cgcacgctgt caggtgtttc atcaaataca aaaaattcat
     7741 attccaatgc ggcataaaca tcatacccca tacccgcagc tttattgatt acacgacgca
     7801 agatcccgcg aggacagatg gcttctgctg cttgtgaaaa ttcagccata aacaacaatt
     7861 gattttcttc aaatagtatt tcacgacagg ttgaaggaac aattcgcact gaagcgtcag
     7921 gataacccgt atgccagcca gtatatttca cattatcata aagcttatct ttagaatccc
     7981 atcctaaaat gacatcacaa aaagcaaaac catgctccaa agccgaaaaa aacttgttac
     8041 ggctcatgta cttgcccagc atcacaccat cgatatcaaa aagaccaacc ttaacatgag
     8101 tcaattgtct ttcttccacg atgcgttttg catcttctat ggttcggacc tctcttgggt
     8161 ttagcatggc atatccttat gtcgttctgt caatgtaagc ttctttacct cccaatgtct
     8221 aattgcggcg aatacgacac tgagacaata acccattttg aatccagttt tttaaataag
     8281 acgctgcttt cattccaacc tcttcagcag gcatccactg gcataatccc tcacaaatcg
     8341 ccccaaaaga ggaaccctga atcagagcgt ctaaagccca agcttcttct tccgacaagc
     8401 tgtaatattg aatgatataa ttttgacccc gccataacac ccagccagta gcagcaggac
     8461 tatctttcaa ctctggaatc tcaagattat tgaccaaagc ttgccacaat gaaaaaacat
     8521 tccaacaaaa atcaagccta tggactgacg aatgaagtac aaaccccatc tcagcccagg
     8581 attcaggcgg cactgctgcc atctcttcta cattaagcac tgtatcatct gctgcatcga
     8641 aggctaaggt cattttccat tccagctcag ccagttcata caaataatag caagacttcg
     8701 catagtactt ttttatcaat tcaggcaatc tatcgccata ccatcggatt gaacggtaag
     8761 cagatgggta agaatcaata taacgagcac ttaacttttc aaattcctca gtacctaaat
     8821 acgcatataa agcggggtaa tttgctgcca aacattcaat caatctcaat cgataaccgt
     8881 ctttataaat atttagacgc gtttctgttg aaactaactt ggttggcact atggactctt
     8941 taattgatcc ctgcccggaa taaatgaatt cttgaaattg ggtttgtaaa gtcaatagcg
     9001 atttcatacg gctacctttt cttttgcagc ttgtatggaa aggcgttttg catgattaat
     9061 ttcacttaaa agttcgctaa aatcaggcat gttatcatca cgttcaatca tagtagatac
     9121 aggcccaagt ctctgtatgg ttgcctcata aagatcccaa accggctcaa tcactggagc
     9181 attgtgagtg tctataatgt aatctccgtg actagtgtga cccgccaaat ggatttgagc
     9241 cactcttcca ggaggcatgg aattaatata agccattgga tcgaaattat gattgaatga
     9301 actgacataa acattgttga catccaacaa taaataacaa ccggattgtt ttactatttc
     9361 cagaataaaa tcccattctg acatttctga ctgtttgtat gtaaggtagc ttgatacatt
     9421 ttcaattaaa aaaggacgtt ttaagaaatc ttgtatttct tgaatacggg atacgatgtg
     9481 ttgaatagct tcagttgtat agggaaccgg taataaatca tgcgtattta atccatgaac
     9541 gccagtccag cataaatgat ctgaaatcca gacaggctca atacgagaag ctaattcttg
     9601 tacctgcttc aaataatccc aattcaaagg atcagtgctt cccagagaaa gagagacgcc
     9661 gtgcatgaca atagaatagt gttcgcgaat tttgtccaga taatagaggg gcttacctcc
     9721 ggggattaaa taattttcag ttaatatttc aaaccaatcc agatcaggtt tgctcgtcag
     9781 gatttcttca taatagtttg gtcgcaatcc taaaccaaaa cctaaatacg gtaatttttg
     9841 atctgactta tttggggata cttccatatt aaacctaaaa aagaatgggg accaggcatg
     9901 tttttctaca tgcctggcaa gatgcgactg aactaattgc tattattcag caacagtacc
     9961 accttgtttt tcgcaatctt ctttagacat ctcaagaata cctttgcctt tgcaagaatt
    10021 ttgacctttg caagcattat cagcagtttt acagctgcct tgacctttac aagcattaac
    10081 acctttacag tgaattgttg tggttgtagc gtcaacagca aatgcaggag ccaaagcaaa
    10141 catagaagca gcgccaacag ctaaagcagc gcctgttaat tgtaatttat tcatagcaat
    10201 tcccttttga ttaatgaaaa tgattgtaaa cccggcaaat ctgaattgtt ttgccaatag
    10261 cattttaaga atcaataatc aaaaaagtca aggttatcca aactgataaa taattatttt
    10321 ttcaaatagt taagtaaatt atttaattac actacaaatt ttgaaagtac tttatatcac
    10381 caactcccgc cgtttaatcg aacaggttcg ggagacaatt gaatcaattt gcgattttct
    10441 aacttccgga tcattaagcg gaattgacct ctaatccttt caataaaagc cattatttct
    10501 gcaccgggaa aaatcacacg ggaatattgc gaaaaaactg gaatatgaat gattgtttcc
    10561 tattttccga gtctctgtag ctcttgttca gaatattaaa tcgaatatta ttttggggat
    10621 tcgttaatcc aaatttactt tttcaagata gttaggtatc ttgcaattcc atcctagctc
    10681 cgcttcaata tgtcccttta accctaatgc cgcttgaggc tcgccatggg taataaaaac
    10741 ttcctttggg gctttctcaa aatgctttag ccaaactagt atttcctcat aatctgcgtg
    10801 agcagaggca ttggttaaca tagctacctg agccctgact ggataatcat cgccatggat
    10861 tttaatggac ggctcatgat ttaacatttt ttcacctctc gttccagcag cctggaatcc
    10921 ggtcaaaagg atagtacttg caggatcagg agcatatacc ttcaaatgat gcaaaaccct
    10981 gccccctgtc atcatgccac ttgcagaaat aataatttta gattcgcgcc cccaatccac
    11041 tgccatggat tcatttgggg tatggacata agtcgctatt tggcagacgg ctcgacactc
    11101 ctctgcactt aagcgatgtt cgttatgatg ttttaataat aattcagtgg catcaatagc
    11161 catagggcta tccagataga caggaacatc aggaattaat tttttttgtt ttaaaatata
    11221 caaataatac aacatagttt gtgccctacc gactgcaaaa gcaggaatta gcactgtacc
    11281 acctctttta caagtcgtat tgactatgtt ggctataaac gctaaaggat cggttttatc
    11341 atgcaagcga ttcccataag ttgattcaat caccaaataa tcggtagatt tcattaactc
    11401 ggaaggcttc ataacaggat cattgagtct tccaatatca ccactaaata ataacttgat
    11461 ttgctttgtt tgaaattgaa taaatgcaga ccctaatata tgccctgccc tatgccaact
    11521 gatttcacaa tcctccatta aaggataagg ggtgtcatat tctactgatt caaaatactt
    11581 taaggtatta tttgcatctt cctgagtata caaaggtaaa gccggatggt gcttgctgta
    11641 cccatattta tttgctcttc tagcatcttc ctcgtgcaaa aaaccgctat caggcagcaa
    11701 gatagaacat aaatcggttg ttgcagctgt tgcatagatg cgccccttaa atccctgttt
    11761 caccaataat ggcaaataac cactatgatc aatatgtgca tgggtcaaaa caacggcatc
    11821 tatcgattct ggagacacag gcaggggtga ccagtttctt agtctcaatt cttttaaccc
    11881 ttggaaaagt ccacaatcta ccaaaatctt tttattattt gattctacca ggtacttaga
    11941 accggtaacc gtactggtag cacctaaaaa agacaatttc ataataatac cttgttgtat
    12001 gtcattctca gtactgtaat gaataacacg acctattgcc aatttatttt aaagtcattt
    12061 gaagtttgaa attgtaaatc tttatcccgc ccattcactt gcacttacgg tgtggaggct
    12121 tttataaaac tgctttttaa gttttttacc cacttgtctc tattctcttt agtcatatcc
    12181 gattttttaa ttgcataatg aagcacataa attccatcct tgcccttggt aattttcaga
    12241 atttcgtctt gctgcagatt ggcaggctct tgaattttaa actcaaaaat gagtagattg
    12301 ggttgctcct caatgacatg gacaatatat tttacccctg atttattcag gttatataaa
    12361 aattgatcgc caaaatgagc tggagtcatg ttgattaaac ccggcataaa ttgtgtagta
    12421 atcaactctt tccagttatt aatgtcatct ccaacaggaa tatactcaat ggtaaccacc
    12481 ttatctgttt tctttcccca agcgatcttc catggtctgt catcaaaacg tactatttgc
    12541 ttctcataat agataacttg tgaatcacct gaggaatcag ccaaaggaac aacactgcaa
    12601 taaagttctc ctttataggt tacaaactcc acacatttag gattattttc tttattgact
    12661 ttgattaatt cttttggaag agaagagcca ttaggctctg cgtagcaaat aacggcatta
    12721 agtaatccaa aacataaccc aatgaaataa ttcatgctct taatccaaaa agtggatact
    12781 atttaattgt agacgttgaa acccatgacc tacaattttg attaggcaaa ctggtatata
    12841 attatattag agagctaata aggagattgc catgaatggt tgcaaaatca gtcctgttgg
    12901 actgggtctt tctttaggaa ttctatgggg attatcattg ttagttatgg gattaatcgc
    12961 ctatttttat gcctatggca ggccctttgt tgaagcagtt gccaccttat atcttggcta
    13021 cgaaccttct gtcctgggaa gcttcattgg tggcgtcatt ggctttattg atgggttcgt
    13081 cacgggtttc cttatcgctt ggctttacaa ttgttttagt tgttgttctt gctgccgagg
    13141 caaagaaaat aaaaaagaat aagcttgcta ataatgagaa atcatgacgt caatgacgtt
    13201 aaaaccaacg ccattgacaa gagcaattaa tttattcatg agttaaaatc atttatgggc
    13261 gaacaataat acggttctct actgactgaa ccccttgaac ttgtctggca atttgttctg
    13321 cgatatcgct ctggcgaatt tttttcacat aaccacttaa tatcaccaca ttttgattgg
    13381 attccacatg aatttgattg atcacaggat cattactttc cattaatgaa tcctttactg
    13441 actgagccaa agtcatgcct gatggattag atggaataaa catgttactc acagtattgg
    13501 tctgacaacc tgctgtaaac aaaatcaaaa acacaatgat tacactggat aaacatttta
    13561 gcatcacaac ctccaagata gagtggattt atttacactg cattaaaaga accaaataat
    13621 agcacaactc cccgtatttc tatagtcaag ctactgtcta taaatcatcg tttgccaaga
    13681 gaaacttaag tcttttcaaa cgataactca gattaaattg tcatttatgg aatgctgcta
    13741 tactattttt tataaatcaa acacctacag gacgcaaggt atgaaaaaaa caaccgaaaa
    13801 acctatcaat aaaacaaaag aaatgcctgc atcctcaaaa aaagaaatcc taaatccact
    13861 ggagcaaaca ccttctcccg aacaatccga tcccattttc agattcattg ataagttata
    13921 tcaagctaac ctgggaaagt taactgctgg tataagtcct gctgcgctag gtacagccta
    13981 ttactcctgg tttgctcaat tgctacagtc acctggttca atgctacgac tggcttatta
    14041 tccgctgttg catgccaacg attatctttc caaccttttc aagtatgaca agcccagaga
    14101 tggcaaggac gtacgttttc atacagaaaa ctggagttat tatccctggc ggctttgggc
    14161 tgagcaattt ctccaatttg aagattggtg tttacaagcc tcaggcaaag ttccaggtat
    14221 accacttcat gtcaaaagaa cagtaacctt ttccaccaga cagattcttg acgccttgtc
    14281 accttccaat tttgttttga ctaatcctga tttgcttcag gaaacaattc gctcgaacgg
    14341 gcaaaattta attcgcggaa cagagcttgc ctttcaggat tttgtagaaa agataacagg
    14401 ctcacctcct gcgggagtcg agaattttat cccggggaaa caagttgctg taacaaaggg
    14461 gaaagtcgtt tatagcaatc atttaattga attaatccag tacacacccc aaacagaaaa
    14521 ggtatacaaa gaaccgatct tgattttacc tgcctggatc atgaaatatt atatcctgga
    14581 tttgctgccg gaaaactctt tagtaaactg gctggttaga cagggacata ctgtatttat
    14641 tgtatcttgg cgcaatccaa cgaaagaaga tagaaatctt ggactggatg attattataa
    14701 gttgggcgct atggatgcca taaatgcagt gagcaatgcc attccacata ccaaaatcca
    14761 tttaatgggt tattgtcttg ggggcaccct ggctctgtta actgcagcag cgatggccca
    14821 tgatcacgat aaccgtctta aaacattatc cttgctcgct gctcaaggcg attttattga
    14881 tgccggagag ctactgctct ttataaccaa aagtgaagta tcctttttaa aaagtatgat
    14941 gtgggagcaa ggatatctgg acacaaaaca aatgtctgga acttttcaaa tgctccgttc
    15001 gtacgatttg atttggtcta aaatggttca ggattacatg cacgggactc aaagagggat
    15061 gatcccttta ctggcatgga atgccgatgc aactcgcatg ccatacaaaa tgcatagtga
    15121 atacctggaa aaactgttcc ttaataatga ttttgccgaa ggccgattta ttctggacgg
    15181 gaaacctgtc gtaggcgaaa atattaggat acctgctttt gttgtaagta cagaaaaaga
    15241 tcacgtagcc ccctggaaat cagtgtataa aacccatttg ttaatcaatt ccgatattac
    15301 ctttgtcttg actaatgggg ggcataacgc tggaattgtc agcgaaccag ggcatgaggg
    15361 acgttattat cgtattcgag aaagaaaaat ggattcaacc tatcttgacc caacaaactg
    15421 gctaaaaaag gctgaattaa gagaaggatc ctggtggatt gcctggcacg attggttagt
    15481 taatcactca tcccaaaaac aagtgtctgc gcccaaactg gacaaaaaat tgcccaatgc
    15541 tcctggaaaa tatgtattac aaaaataaag gaattagtaa tgaataatga atttcttgaa
    15601 aatgtcacct atgaagaact gactataggg cgcaaggcca gcttaaaccg tacattaact
    15661 caggatgata ttaatttatt tgcaacaatg tctggagaca tcaatccatc tcatgtcgat
    15721 cctcaatttg ctaaaagcga tgtattccat ggcgtagtcg gacatggcat gtggtctgga
    15781 gctctaattt ctaccttatt gggaactgcc cttcccgggc ctggaactat ctatcttgaa
    15841 caagatattc aatttataac tcctgttcgc attggggaca atcttacaat taccatcgtt
    15901 gttcgtgaca aacatccagg caaatcgatt gttcatttcg actgcatggg agtgaatcaa
    15961 aaaggagaaa cagtaattaa aggccttgcc aaagtcattg ctccgactaa aaaaatgaag
    16021 gtacctcgaa ctgttttgcc tctggtgcaa atccatcaca atgaccactt taataaaatc
    16081 attgaagcct gcagtaactt gccctcaatc aaaacagcag ttgttcatcc tgtcactgcc
    16141 aacgtcctgg aagcagttta tgactcagtc aaggctggtt taattactcc tgtactaatt
    16201 ggtcctatgg caaaaatcaa atgtgccgca agtgaggcta aaatcgattt atccaattgg
    16261 gaaatgattg atacagaaca cagtgatgct gctgctttta gagcagttga attagcggca
    16321 tccgggaaag tggatgctat catgaaaggc gccctgcata ctgatgagtt aatgtctgca
    16381 gtggtacctg ccacttctgg actaagaacc aaataccgca tcagtcacgc ctatgttatg
    16441 gatgttccaa cttaccataa acctttaatt attaccgacg cagccattaa tatagcccca
    16501 aacgcagcgg ataaagcaga tatatgtcaa aatgcaatca atctctggcg tattttatat
    16561 ggagaagaaa taaaacccaa ggtagctatc cttgcagccg ttgagatggt aaatccaaag
    16621 atgcaggcaa cagttgatgc cgctatcctg tgtaaaatgg ctgaccgagc tcagatagtc
    16681 gatggcatac tggatggccc cctcgccttt gataatgcca tcaataaaca agccagtaag
    16741 gaaaaaaata ttatatctcc tgtagccggc gatgccgaca ttttgctggt accggatatt
    16801 gaatcaggaa atatgctagc caaacaactc acttttctcg ggcatgctga cgcagcagga
    16861 atagttctcg gagcaagagt ccctatcatt ctggccagca gagctgattc gttaagaaca
    16921 cgtttgttat cgtgtgcggt agcaaccaag atagcagcgg cccgtaaagc aggcaaaata
    16981 aaatgaggca ttccgcactg ttaattatca attcaggttc ctcaagtgtt aaatttcaag
    17041 tgttctccaa tacagagaaa cttgttcttc tcgccaaagg caagattatt aatattggag
    17101 gagaaccatc ttttaccgca gttaacaaaa cctcagagca tcagaacact ctaatcactt
    17161 tggaaaagga catgacgcat gagcaagcca ttcatcttat acttgactgg attagccaac
    17221 aacaacaaaa ctgggaaatt catgctgtca cccaccgggt cgttcatggc ggagaatact
    17281 tcactcagag cgtcctggtt actccacaaa tcatcagcca attggaaaca ctgaattctc
    17341 tcgcgccact gcatcagcct cataacttgg ccgccataaa aatcattaag aagattaagc
    17401 cggaattacc tcagattgca tgctttgata ctgcttttca tgctcaacat actccattat
    17461 ttactacctg tgctttgccc gaatatttaa gacaacaagg gattaggcgt tatggatttc
    17521 atggtctttc ctatgaatgg atttctcttg ttctcaaaaa agactaccct gatttggcta
    17581 acaatcgaat tattattgcc catttaggca atggcgccag cttatgcgct atggataagg
    17641 gagtcagtat agacaccacc atgagtttga cagcactgga tggtttacca atgggaactc
    17701 gttgtggcag tttggatcct ggggctgtta tttacatgat gagagaattg aatcttccac
    17761 cagataaggt agaggaaata ctttataacg aatccggctt gcttggttta tcaggaaaaa
    17821 ccaataatgt gctggaatta caatccagcc ctgataaaaa cgcccaattt gctttggagt
    17881 atttttgcct gaaaacagct caatttattg gaatgatgac catttcgcta ggtggagtag
    17941 atggtattgt attcacagga ggaatagggg aaaattcaga accgattaga gcaaatatcc
    18001 tcaagaggat agaatgctta cacccttttt ctgctatcgt tattccagcg aatgaagaaa
    18061 aaatgatggc tatgcatacc ttgacagccc tcaggacgta aaggcgtatt ttttactggc
    18121 ataggtaatg agtataaaaa atggataaag actactataa aatcatggga gtcagtcagg
    18181 atgcttctga aaaagacatc aaaatggctt accgaaaatt agcccgcaaa tatcaccccg
    18241 atattagtaa agaaccagat gctgaagaac gatttaaaga aatggcagaa gcctatgaag
    18301 tactgaaaga ccctaaaaaa agatccgaat atgatcaata tctgaaatac aaggaattca
    18361 atccccaaaa cgatggattt accgggcgaa gaactcaaga acaacccttt caggaatttc
    18421 attttgacag cgattttttt gaaaccttgt ttggacattc gcgctatcaa cagcaaccga
    18481 tgactggtca aaactatcat ggcaaaatca ctatcagtct ggaggaagct taccatggag
    18541 ctgttaaaaa cttacaagtt cctgttgaac aaggcacaga gactaaaatg cagaccctta
    18601 aagttaaaat ccctgcaggg gttaaatcag gacaacaaat tcgcttggca ggacaaggag
    18661 gcagtggaag cggaggaggt gctcgcggcg acctgtatct aacagtcgag gtgataaaac
    18721 accccatatt tgatgtcatg gagaatgata tttatctcac attacctatc acaccgtggg
    18781 aagctgcatt aggagctacg gttgtcatac caaccctggc aggaaaaatc gatttaaaaa
    18841 tccctccggg ctcacaaggt gggcaaaaac tcagaattaa aaatcgcgga ttaccaggtt
    18901 caccccctgg taatcaatat gttctattaa aaattattac cccccctgct caaactgaag
    18961 aagcaaaagc tttatataaa aaaatggcgg aggtaatgcc ttacaaccct cgtaagacta
    19021 tgggagtata agcatgagca aagataatct taaagacaat cttttaatag gcatgcttat
    19081 tgaagaaacc acaacgattt cttttattga agtatgtcaa aagtatcata ttcctgaaga
    19141 attattcatt gaaatgatcg aacaaggttt gtttccagat caaccatctg atcccgaaaa
    19201 aattgcatta ggccaaaaag aactgcgtcg ccttgaatcc gcctttcgat tacatagaga
    19261 tttaggcatt aacctgcctg gagtcgcttt agctcttgat ttactggaaa aaattgatca
    19321 aatgcatcat gagcttgaaa ttttacgcaa gcatttttag gcatcagaat ctttaggatt
    19381 ataatcaggt atctctttag gacgataacc tttaatcgtt ctaaaaaact cgataatgta
    19441 ctgaaaatcc tttttaatat ctcctgtggt ccaaaaaggt tcatggatac ggatttcttt
    19501 agtggaataa tcaggcccca ccatcactat aggaatacct gcttgccgag caatatgata
    19561 aaacccctct ttccatctgg tcaccggact tcgggtacct tcaggagcca ggcccagctt
    19621 taattcatcg cgttggcgaa acaactctac aactttctca accctgttcc cctttttaga
    19681 acgatcaacc ggggtgcctc caactgcacg caaaaaatag ctcaaaggga aaaaaaataa
    19741 ctgatgtttc gctaaaaaat tgattttaac cccgacaaca aaacgggcac acaggcaaat
    19801 gacaaagtcc caattgctgg tatgcggagc aacaatcaca atatattttt tttcattcgg
    19861 taattcacca actatcttcc aaccgattaa tttaaaaata aaacgacata ttttttgcat
    19921 gacaaaaatc catttttgtt ttaacca
//
LOCUS       DATNSN010000046        19686 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.046, whole genome shotgun sequence.
ACCESSION   DATNSN010000046 DATNSN010000000
VERSION     DATNSN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19686
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.046"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(32..1375)
                     /gene="nadA"
                     /locus_tag="VAH76_001367"
     CDS             complement(32..1375)
                     /gene="nadA"
                     /locus_tag="VAH76_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEO1386002.1"
                     /translation="MFKNSTTYQPEATLMAIRNDMAICQSDYPLDWYQEDFIPYAQEY
                     QALPDRKLTTVLNWMKPYLAKAQNHFGNKLLLLAHYYMGGEIVRLIEQFGGKIGDSYQ
                     LALMAANNPEKSVIVESAVHFMAESISILANENQHVYITNPKSGCTMEMLAKDFMVEP
                     AFLDLNERYGAENILPVCYMNTSGRVKAMTGAQGGAVCTSSNVKKIFQWAQKQNKKIL
                     FIPDQHMGENVAYWLGIKNLAYWPGGTAGANYSLPAQDKKTLKQFDKAELILFASQCA
                     VHTHYQPEMCDYWKSLGYTTIVHPECRNDVIKVADYSGSTAFIWDLVVHDKAGTKSYA
                     IGTENHMVENLKQYCKNLGIRVVNLAEAPQKDTEKGVGCGCATMSRNDPPHLVALLDL
                     LRQGKSMPYNEVKAGDVVNEFTGTRNRLPVNDQQWVINNAKKALEMMINITEDRV"
     gene            complement(1450..3099)
                     /gene="nadB"
                     /locus_tag="VAH76_001368"
     CDS             complement(1450..3099)
                     /gene="nadB"
                     /locus_tag="VAH76_001368"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEO1386003.1"
                     /translation="MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPKLNIALI
                     SKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDII
                     KQLDQYKVQFKRENDGRFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
                     FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQHNRIHTFLAKCVILATGGAGKTYRY
                     TTNPMVATGDGVAMAYRAGARVGNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKN
                     PETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQ
                     IYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGAN
                     RLASNSLLEALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRG
                     LRGEMTSYAGIVRTEAGLEDLLQLILKRKKIIEEYYWKHCITRDFIELRNIILNAELI
                     VRAALSRRESRGGHFREDFPNKSFKAQESIAKLGQADSSLG"
     gene            complement(3096..4466)
                     /gene="purB"
                     /locus_tag="VAH76_001369"
     CDS             complement(3096..4466)
                     /gene="purB"
                     /locus_tag="VAH76_001369"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO1386004.1"
                     /translation="MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWF
                     ESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQD
                     KFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
                     HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHV
                     AAYPEVDWRKHCANFVTSLGLSFNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIW
                     SYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQ
                     RDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTV
                     MRRYNEPNAYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQ
                     LVKAFS"
     gene            4619..6322
                     /gene="sulP"
                     /locus_tag="VAH76_001370"
     CDS             4619..6322
                     /gene="sulP"
                     /locus_tag="VAH76_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEO1386005.1"
                     /translation="MNTITESYRAGLFQKKYWLQNIISGLIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGLIVSIMGGSRVQIAGPTGAFIVVLSGITAQYGISGLQIATLMAG
                     FILVLFGFARLGSIIKFIPSPVIIGFTSGIAVVIWVGQWQYFFGLPAAGSGHFHEKFW
                     HLLLSFPHMNIATTFLGICALFIVLYSSKLPGLKRIPGPLIALVLVTIIQSVWHFEGV
                     ATIGSLFGGIPQGLPAFTWPEITMGRLIELIGPAFAIAMLGAIESLLSAVVADGMSGT
                     RHNSNRELVGQGIANIVAPLFGGFAATGAIARTATNIRNGGNSPLAGIIHSITLILVL
                     LFLAPLAVDVPLAALAAILFVVSWNMSEARHFIKLIRVAPRADVVILLVTFCLTVFVD
                     LVVAVNVGVIIAVLHFLRRMVNSIQIQRMSEGQLSQELAQEKNYDLPEGVMVYVIEGP
                     FFFGAVETFQSALANTHTDPKVLIIRMRWVPFIDVTGLQAMEEIILNLQKRGVRVLLS
                     EANPLVDAKLRKMGIVQLIGEQNYYKEFSQALDVCRINDSDRKSQPPFEVAKPLASVT
                     Q"
     gene            complement(6525..8228)
                     /locus_tag="VAH76_001371"
     CDS             complement(6525..8228)
                     /locus_tag="VAH76_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEO1386006.1"
                     /translation="MSIKNEYETARDHFRLWDKQLKDNILLEKSCLMHTYKTYFPDEP
                     GFIQQLSQFSKAVAEELEPTVMENNLDANLPKIEQYNAIGQRDDRVIHHPAYVRSGDI
                     IYGSNLMQYLLKPGQMKKTLSLFLLSSHAGEAGHNCPIACSAGVIRVLSRYSQLEKTS
                     YYLDKLTHPSFSYNFTGAQFLTEIQGGSDVGANATRASLDKQNQWRISGEKWFCSNAN
                     ADLILMTARFDEETPGTKGLGLFLVPSRLDDGSPNHYYLRRLKQKIGTRTMATAEINF
                     EGALAYPMGDVAEGIHLVMENVLHLSRIFNSFSVLGMSRRAYQIACYYAKNRRAFNHP
                     IIEYPLVKESLAKIKSENLAMLASVFHMARCQDELDALPEPQQTKSQQLLLRTLANIN
                     KYFTAKRSVDNIHHCLDLLAGNGTIENFSSLPRLLRDCIVCENWEGTHFTLWMQTLRD
                     MHKFAIDELFIAHLQQLLEQVQDINPHKALFFEKINELDTTIKTMKLANHEEQSLHIK
                     TIIEHMASLLAALCLAIEIQQGQSPETKQAGLTLFIHDHIASCLLQHDDYMKILNKVI
                     G"
     gene            complement(8457..9455)
                     /locus_tag="VAH76_001372"
     CDS             complement(8457..9455)
                     /locus_tag="VAH76_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEO1386007.1"
                     /translation="MFKRIGLISIALSLNTISLTSEACSALVFNKNKPATVAVNLDWK
                     YREGAFVIHPRNAMMVSSVDYHSMHPLIWKSQYGSVIFHGGNRFKAGPSADGMNEKGL
                     TASILMLDSSSYPSNSELPALKTTEWVQYILDNFQTVQEVIDDSSNYQLVADTYRGIT
                     MNVHLMVSDAEGKSAVFEYLDGKLVIHTQDNLTTPVLTNTDYKSSLALLEEYQDHGGS
                     KALPGGYDSKSRFVRAAHYLKKLPSFIAKEEHIAYAFNGLNVVTQAPGTSSPTQLSMV
                     FDIPTKTIYFRSINEAALRVIPMDSIDFDYLYQPIALNVYQHLSGDVTDKFQPMIG"
     gene            9680..12067
                     /gene="ppsA"
                     /locus_tag="VAH76_001373"
     CDS             9680..12067
                     /gene="ppsA"
                     /locus_tag="VAH76_001373"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEO1386008.1"
                     /translation="MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPG
                     GFATTADSFREFLSQNGLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFE
                     QSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
                     QVFASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVV
                     FITSSYGLGEMVVQGAVNPDEFYVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLER
                     RVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWAKDGVNGKLYILQA
                     RPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDV
                     LISDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDV
                     VTVSCAEGDTGFVYSGLLPYEQERLDVETMPELPMKVMLNVGNPERAFAFQSIPNSGV
                     GLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERLKEGIA
                     TIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECF
                     ALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCE
                     LPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLVASQFDERNDAVKALLHMAI
                     AACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN"
     gene            12308..13747
                     /locus_tag="VAH76_001374"
     CDS             12308..13747
                     /locus_tag="VAH76_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1386009.1"
                     /translation="MSNSKWWFLLVCFIPAITFASEGGEHSDPVTSVLLGVTTILFLA
                     IIGRYLARLLNQPGVLGELLIGMIVGNLSYFFGSELVVILREGSAIYDVVKEMLAGIS
                     LNQAVTHVIHNSYYANQVIHALSGAEGTEYLKIAYVVDIFSRYGVIFLLFMVGLETSL
                     SDLKKTGKESILVAVIGIVAPMILGFVVACFIIPNSNYKINLFIGATLSATSIGITAS
                     VLKEMNKLSTREARTILGAATLDDILGLIILAVVSSIVISGAVSIMVVMKIIILALLF
                     FIGTLFMGPFVLRKAVDLFRFLEPWESKLFISFLFIMALAWLASFIQLAAIIGAFAAG
                     VILHDDYFGEQKAHHKDHRSIHHLVSPLETILAPMFFTLIGIQVKLETFYDWNVIVLA
                     GGLSVAAILGKLLSGLGANRRDDRLLIGIGMLPRGEVGLVFASIGRTIGVISDNLFTA
                     IVLMIMITTFIAPPLLKMRYAKPKEDRNA"
     gene            13744..14586
                     /gene="nadC"
                     /locus_tag="VAH76_001375"
     CDS             13744..14586
                     /gene="nadC"
                     /locus_tag="VAH76_001375"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEO1386010.1"
                     /translation="MNNVLSQVTFDVKRALQEDVGNGDVTAALLPQQLIVEAEIISRE
                     PMLVCGQPWVNEVFKQVDNTVEMEWRVSEGDFLSTPATLCIIHGTASSILTAERTALN
                     FLQTLSATATQTYHYVQKLKGTKAKLLDTRKTIPGLRLAQKYAVHCGGGLNHRMGLYD
                     AFLIKENHIKACGSVAHAISLARKTDKHLLVEIEVETLEELQEALDAHPDRILLDNFT
                     QDMLEQAVKMNHPKYCELEASGGININNIADIAQTGVDFISVGSITKSIQAIDLSLLI
                     RETL"
     gene            14583..15674
                     /locus_tag="VAH76_001376"
     CDS             14583..15674
                     /locus_tag="VAH76_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEO1386011.1"
                     /translation="MSPSIVFTGGGTAGHVTPNIALIKEFRKEGWNVEYIGSVSGIEK
                     EMIEPMDIPFHGVSSGKLRRYFSLKNLLDPFKIVLGIIQSSLLFYKIKPDVVFSKGGF
                     VAFPVVVGAWLNRIPVVAHESDMSPGLANRLSFPFVNKICLTFDAGKKYFKRQDKIEV
                     TGTPIRQQLLTGNRMKGLELCGFNSSKPCLLVVGGSLGAGSINSCIRSALKQLTSEFQ
                     VIHLCGKGKLDSSLVGVEGYCQFEYANEELADLFAASSVVISRAGANSLYEILALGKP
                     HILIPISSQVSRGDQIQNARYFQGLGISVVIQDELLKADVLLQAVQDVMRKKDEIDNK
                     IKALKIESATDKIVAIIKEQAHVQTPRIV"
     gene            15649..17064
                     /locus_tag="VAH76_001377"
     CDS             15649..17064
                     /locus_tag="VAH76_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEO1386012.1"
                     /translation="MFKPQGLYDYICQQWQEEILPSLCDYIKIPNKSPHFDAKWEEHG
                     YMEQAVNHIANWCKSHAPKGMTLEIVRLKNRTPLLFMEIPGQIDDTVLLYGHLDKQPE
                     MSGWSDDLHPWKPVLKNGLLYGRGGADDGYSAYASLTAIRALEQQGLPYPRCILIIEA
                     CEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELI
                     NEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEI
                     LGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTS
                     LKLSMRLPPLVDPEAASVAMEKALTQNPPYNAKVDFKIQNGGSKGWNAPLLSDWLAKA
                     ASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHSNAHGPNEFLHL
                     DMVKKLTSCVSYVLYSFSQKK"
     gene            17156..17473
                     /locus_tag="VAH76_001378"
     CDS             17156..17473
                     /locus_tag="VAH76_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO1386013.1"
                     /translation="MKARLIAILLSFIVIPFSSQAENEPQKTPAKQVLVKGKINLNTA
                     DVHSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEELAEVKGIGQRFVDKNREKLKEIF
                     VIN"
     gene            17669..18706
                     /locus_tag="VAH76_001379"
     CDS             17669..18706
                     /locus_tag="VAH76_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEO1386014.1"
                     /translation="MFRKLRGVFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVALRNE
                     SGQKRVAAVGLEAKRMLGRTPGNINAIRPMKDGVIADFFVTEKMLQHFIHKVHENKFL
                     RPSPRVLVCVPCGSTQVERRAIRESAMGAGAREVFLIEEPMAAALGSGMPVEEASGSM
                     VVDIGGGTTEVAIISLSGIVYHQSVRIGGDKFDDAIVSYVRRNYGTLIGETTAERIKH
                     EIGSAFPSRDLFEIEVRGRNLAEGVPRSFTLTSAEILEALQEPLSGIVGAVRAALELA
                     PPELAADIAERGMVLTGGGALLKNIDTLLMEETGLPVLIAEDPLTCVARGGGKALETM
                     DLRGGDFLSTE"
     gene            18684..19592
                     /gene="mreC"
                     /locus_tag="VAH76_001380"
     CDS             18684..19592
                     /gene="mreC"
                     /locus_tag="VAH76_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEO1386015.1"
                     /translation="MISSQQNNKHNRLFAKGGHSSLGFVFALAFSILLMFSDYHYRYL
                     DFVRSGFSLIVSPLQYAVDYPVRVIGWIQSLVSAKKALIDENMRLKYKQTMLEAELQK
                     LIVIQRENSQLKELLLTSSKADMRVMAAQILAVDTSQARQVVVLNKGTRDGVYVGQPV
                     LDAKGVMGQVIDVGPMTSTLLLISDSKSAVPVRNNRTGERAILVGTNDIEELSLINLP
                     KTSSIHPGDVLVTSGLGRRYPEGYPVGRVEEVKSIPGEDFVKVTVSPVALLNRNRLVL
                     LIWADSEQEELTAQINERLNAEESTA"
BASE COUNT         5480 a         3610 c         4408 g         6188 t
ORIGIN      
        1 aggaagcgga caatacacta gctaacacgt attaaaccct gtcttcagtg atattaatca
       61 tcatttccag ggcttttttg gcattattaa taacccattg ttgatcgttg actgggagtc
      121 tgtttctggt tcctgtgaat tcgttgacca catcacctgc ttttacttca ttgtaaggca
      181 tcgatttgcc ctggcgaagt aaatcgagga gagcgaccag atgtggcgga tcattgcgag
      241 acatggtagc acaaccacat ccaactcctt tttcagtgtc tttttgaggg gcttcagcaa
      301 gatttacaac acgaattcca agatttttac aatattgctt taagttttct accatgtggt
      361 tttcagttcc gatggcataa ctttttgtac ctgctttgtc atggactacc agatcccata
      421 tgaatgcagt agaacctgag taatcagcca ctttgatgac atcattacgg cactctggat
      481 gtacaatggt agtgtatccc aggcttttcc aataatcaca catttcaggt tgataatggg
      541 tatgaaccgc gcattgactt gcgaataaaa tcaactctgc tttatcaaat tgcttgagtg
      601 tttttttgtc ttgcgctggt agtgaataat tagcgccggc agttcctcca ggccaataag
      661 cgagattttt aattcctaac cagtaagcga cattttctcc catgtgttga tcaggaataa
      721 acaggatttt cttgttttgt ttttgagccc attgaaaaat ttttttgaca ttggaacttg
      781 tacaaaccgc acctccctgg gcaccggtca tggctttcac acgaccagag gtattcatgt
      841 agcacacagg taaaatattt tcagcaccat aacgctcatt taaatcaagg aaagcaggct
      901 cgaccataaa gtctttggca agcatttcca tggtgcagcc tgacttggga ttggtgatgt
      961 agacgtgttg attttcatta gccaaaatgc ttatggattc cgccataaaa tgaactgctg
     1021 attccactat gactgatttt tcagggttat tcgcagccat caaagccaat tggtaggagt
     1081 caccgatttt acccccaaac tgttcaatta atcgaacaat ttctccaccc atataataat
     1141 gggcaagaag taacagtttg ttaccgaaat gattttgggc tttagcaaga taaggtttca
     1201 tccaatttaa aacagtcgta agctttctat cgggtaaagc ctgatattcc tgggcataag
     1261 gaataaaatc ttcttgatac caatctaatg gataatcgct ttgacatata gccatatcat
     1321 tacgaatagc catgagggtc gcttcgggtt ggtaagtagt tgaattttta aacattaaat
     1381 ctacctctag tcattaggga tttgtgggaa ggattctttc attattgtta atgggatagc
     1441 tttgccaggc tatcctaatg aactgtctgc ctgtcccagt tttgcaatac tttcctgggc
     1501 tttgaaactt ttattgggga aatcttcacg aaaatgtcca ccacgtgatt cccttctgga
     1561 aagggctgct ctaacaatca attctgcatt aaggataatg ttccttaact caataaaatc
     1621 tctggtaatg cagtgtttcc agtaatactc ttcaataatt ttcttacgct tcaatattaa
     1681 ttgtagtaga tcttcaaggc ctgcttcagt gcgcacaatg cctgcatagg aagtcatttc
     1741 ccctcttaag cctctccatt gagcgttaat ttggctggct cttcttgcat tgacttcacc
     1801 tggagagctc cagctaggga tatgatcgat aagtttcagc ggagtagcaa tgtctttgag
     1861 tgtacagcgt gccgcattgg aacccatgac caaggcttca agtagggaat tgcttgccag
     1921 cctgttcgca ccatgcagcc cagtaaaagc cacttcaccg atagcgtata atcgtgccag
     1981 atcggtgcgg ccgtccacat cagtaagaac cccaccgcat tggtaatgag cggccggaac
     2041 gacaggaatc aaatcctggc tcatatcaat tccaatagat aacaatgtgg aataaatttg
     2101 tgggaaacgt tttttcagga aagatttgct ttgatgagaa atatccagat gcacatatcc
     2161 agcctggcct tgctcaattt cactaaaaat agctcttgcc acgacgtctc tggttgctaa
     2221 ctcaagttgg tcaggtgcgt atcgtttcat aaaacgctcg cctgtttcag ggtttttaag
     2281 caaagcgcct tcgcctcgaa ctgcttcgga aattaaaaaa ttgttaatgg tgtgatggtg
     2341 caagagggta ggatgaaatt gataaaattc catgttgccc acacgagccc cagccctgta
     2401 tgccatcgcg acaccatcac ctgttgcaac catagggtta gtcgtatacc tgtaggtttt
     2461 gccggcgcca ccggtcgcta aaatgacgca tttcgcaaga aaggtgtgaa tgcgattatg
     2521 ttggcagtcg agtatatagg ctcctaatac ctcaccctgg ttatctgtcc ggtgaggatg
     2581 atattgagta atcaggttaa ccgccacgtg atgttcaaaa aactggatct gttgatgttg
     2641 ccttgccaaa tgattgagag tttgtgttag cgttaatccc gtttgatcgc cacaattaaa
     2701 aatgcgccgg tgtgaatggc caccttcttt ggcaagatga aagcgaccat cattttctct
     2761 tttaaattga actttatact gatcaagttg ttttataata tctggccctt ggcggataat
     2821 aaattccacc gcaggtaagt agcatagccc atctccagcc aatacagtat cagatatatg
     2881 agactccagt gaatcttctg gcgaaatggc ggcagcaatc cccccttgag catagcggct
     2941 gttacactca atggactctg ctttgctgat taacgcaatg tttagcttgg gttggatagc
     3001 taaaagctgc aaacaataat gtaacccggc taatcctgtt ccaatgacga gaacatcgaa
     3061 ttcgaacgat tggccttgtt gtgatagctg atcactcatg aaaaagcctt gaccaattgg
     3121 gttgcaagac ctgtgtaagt ctctggagtt aatttcatta actctgcttt tgcttcttcg
     3181 ggaatagaca gggttttaat aaatttttta aggttttcag cgtctatcat ttgtcctcgt
     3241 gtaagctctt ttaattgttc ataggcattg ggttcattgt agcggcgcat gacagtttgt
     3301 atggcctctg ctaaaacttc ccagttttcg gataaatctt tttgtagagc tgatttattg
     3361 atttgtaatt tatcattacc tttggctact gagtgatagg caatcaaact gtaagagaag
     3421 gctacgccca agttacgtaa gacagtcgaa tcagacaggt ctctttgcat gcgtgattga
     3481 gttaatttgt tggcaaaatg aataaataaa gcattagaca gacctaaatt gccttcggca
     3541 ttttcaaagt ctataggatt taccttgtgc ggcatggtcg atgagcctac ttcttcggca
     3601 attgtttttt gcttaaagta gccaagagaa atatagctcc atatgtcttg ggtataatcc
     3661 agaagaatat tattaattct caccataatc tgggagactt ccgctatgcc atcatgtggt
     3721 tcaatttgag tggtataggc gttaaaggat aaacctaggg atgtaacaaa attagcgcaa
     3781 tgcttacgcc agtccacttc gggataggct gctacgtgag cattgtaatt accaacagct
     3841 ccattaaatt tggcagggat taatacttct gccaattgtt gctggggtcg tttaaggcga
     3901 gctacaaaat tgaccaattc tttacccatg gtggttggtg ttgctggttg accatgcgtt
     3961 cttgagagca tcgcaacgtc agcatgttgt tttccaagaa gcgtaatgct tcccatgatc
     4021 tcagctatag taggttgtat aacttgggct atcgcttgtt tgatcattaa agcataagcc
     4081 aggttattga tgtcctctga agtgcaggca aagtgtatga aagcaacgca agatttgagt
     4141 tgttcatttt cctgaaattt atcctgaaga taatactcta cagctttaac gtcatgattg
     4201 gtttgttttt caaattcttt tattttttca gcttctgatt cattaaaatt ggaaataaga
     4261 tcagataaaa atttacgagc cttgttatcc aaagcaggta cttctggaat agtgtcgtta
     4321 gcagctaatg attcaaacca cttgatttca accattaaac gataataggt taatgcaaac
     4381 tcactaaaat agggacttaa tgctctggtt ttattgacat aacgaccatc tatgggtgaa
     4441 atcgcgttta atgcagtaag tgtcatgata ggtagcttat tccaatatta aagcgtatat
     4501 aatattagat gcaggacaag atgtgaatta aagcgataaa tattctagaa aattagcgta
     4561 aatttgttca aagtgcgtat aataatgacc atgtttttaa tgggacaggt tattatttat
     4621 gaacacgatc acagaatcgt atcgagccgg tttatttcaa aaaaaatatt ggttacaaaa
     4681 tattatttct ggcttaattg ttggtgttgt tgctttacct ttggcaatgg cttttgcaat
     4741 agcttccggg gctaaaccgg aacaaggatt atataccgct atcgttgccg gattaatagt
     4801 ctcaattatg ggtggcagca gagttcaaat cgccggtcct acaggtgctt tcattgtcgt
     4861 tctttccggg ataacggccc aatatggtat ttcagggtta caaatagcta ctttaatggc
     4921 gggctttatc ttggttctgt ttggctttgc ccgtctggga tccattatta aatttattcc
     4981 cagtccagta atcatcggat ttacttcagg gattgctgtc gttatttggg tagggcaatg
     5041 gcaatatttt tttggtttac ccgcagctgg aagcggacat tttcatgaaa aattttggca
     5101 tttacttctg tcttttcctc atatgaatat cgcgactact tttttaggta tttgtgcttt
     5161 atttattgta ctgtattcca gtaaattacc aggactaaag cgtattcctg gccctttgat
     5221 tgctttggta ctggttacaa tcatacagtc agtttggcac tttgagggag tggccactat
     5281 aggcagcctc tttggaggaa taccacaagg cttgccggct ttcacctggc ctgaaatcac
     5341 aatgggacga ctgattgagt tgattgggcc agcctttgct attgcgatgc tgggagcaat
     5401 tgagtcctta ttgtctgctg tggttgccga tgggatgagt ggaaccagac ataattcgaa
     5461 tagagaatta gttggtcaag ggatagctaa tattgtggcg cctttgtttg gtggttttgc
     5521 tgccaccgga gcgattgccc gcacggccac aaacatacgc aatgggggca atagtcctct
     5581 ggcaggaatt attcacagca ttactttgat tttagtcctg ttgttcctgg ctcctctggc
     5641 agtggatgta cctctggcgg cattggctgc tattttattt gtggtttcgt ggaatatgag
     5701 tgaagcccgc cactttatta aattaattcg agtagctcct cgtgctgatg tagtcatctt
     5761 attggttact ttttgtctga cggtgtttgt tgatttggtt gttgctgtca atgtaggggt
     5821 catcattgcc gtcttgcatt tcctgcgccg aatggttaac agcattcaaa ttcagcgtat
     5881 gtcagaagga cagttatctc aagagttggc acaagaaaag aattatgatt tgcccgaagg
     5941 ggtcatggtg tatgttatcg agggcccgtt tttctttggt gctgtcgaaa catttcagag
     6001 tgcgttggct aatacccata ctgatccgaa agttttgatc atcagaatgc gttgggttcc
     6061 gtttatagat gtcactggtt tgcaggctat ggaagaaatt attttgaatt tgcagaagcg
     6121 tggagtcaga gttttgctca gtgaggctaa cccgctggtt gatgcgaaac tccgtaaaat
     6181 gggaattgtt caattgattg gagagcaaaa ttattataag gaattttctc aggctcttga
     6241 tgtttgccga attaatgatt ctgacagaaa gagtcagcct ccctttgaag ttgcaaagcc
     6301 gctggcttca gttacccagt gagctaagct ggttatctgt tcaagttgag gaagcgcttc
     6361 ctcaactcga atggatagaa ttaacgtgat aatcgaaaaa cttcaaagat tgccatgcct
     6421 gtttttatat gtaattcaat ttgctaaact taaaatctat ttttttggtt tgacactacg
     6481 aataattttt cccggcctgc tggttaatgc atttgcagag tgaactatcc aattacttta
     6541 tttagtattt tcatatagtc gtcgtgttga agtaaacaac ttgctatatg atcatgaata
     6601 aataaagtta atccagcttg ttttgtttct ggcgactggc cttgttgtat ctctatagcc
     6661 agacataaag cagcaagcaa cgaggccata tgttcaatga ttgtttttat atgcagactt
     6721 tgctcctcat gattggctaa tttcattgtt tttattgttg tatctaactc atttatcttt
     6781 tcgaaaaaca atgccttatg agggttgata tcctgaactt gttccagaag ttgttgcaaa
     6841 tgggcaatga ataattcatc aattgcaaat ttatgcatat ctctgagggt ttgcatccat
     6901 aatgtaaaat gcgtcccctc ccaattttcg caaacgatac agtctcttaa tagtcttggt
     6961 aatgaagaaa agttctctat ggtgccattt cctgccaata aatcgagaca atgatgtata
     7021 ttgtccactg aacgtttggc tgtaaagtac ttgttgatat tcgccagtgt acgtaataag
     7081 agttgttgtg atttggtttg ttgaggctct ggaagagcat ctaattcatc ctgacaacga
     7141 gccatatgaa acacgctggc taacatagcc agattttctg attttatttt agccaaactt
     7201 tcttttacca gaggatattc aataatagga tggttaaatg ctcggcggtt tttagcataa
     7261 tagcaagcaa tctgataggc gcgacgagac atacccaaga ctgagaagga attgaatatc
     7321 cttgataaat gcaacacatt ttccataacc aaatgtattc cttccgccac atcacccata
     7381 gggtaggcta gtgccccttc aaaatttatt tctgcggttg ccatggtgcg ggtaccaatt
     7441 ttttgtttta atcgacgaag ataatagtga ttagggctgc catcgtccag gcgactgggc
     7501 actaaaaaca aacccaaccc tttggttccg ggagtttcct cgtcgaaacg agccgtcatt
     7561 aaaattaaat cggcattagc gttggagcaa aaccattttt cacctgaaat gcgccattga
     7621 ttttgcttat ccagactggc acgggtcgca ttggcgccaa cgtcagaacc accttgaatt
     7681 tcagttaaaa attgggctcc agtaaaattg taactgaaag aagggtgagt gagtttatcg
     7741 agataatatg aggttttctc taattgggaa taacgactta aaacacgtat tactcccgca
     7801 gaacaggcaa tagggcagtt gtgtcctgcc tccccggcat gcgatgagag cagaaacagg
     7861 ctcaacgttt ttttcatttg ccctggcttt agtaaatatt gcatgaggtt ggatccataa
     7921 ataatatcgc cggatcgaac ataggcagga tggtggatta cccgatcatc gcgctgacca
     7981 attgcattgt attgttcgat ttttggcaag ttggcatcca ggttattttc cataacggtt
     8041 ggttctaatt cttcagctac tgctttggaa aattgactca attgttgaat aaaaccaggc
     8101 tcatcgggaa aataagtttt gtaagtgtgc attaaacaag acttttcgag cagtatattg
     8161 tctttgagtt gtttgtccca taacctgaaa tggtctcttg ctgtttcata ttcatttttt
     8221 atgctcatta ccccaccctt tggtaattaa tagatgcatt gatatcacag ttttttaagt
     8281 tcttgttgta cttaaatatt tttcaataaa ttgtaatagc ggttattaat caattatccc
     8341 cctcggcttc gtttgtgcca tatccatgcg gcttcacaat agacctcttg cctaaacttg
     8401 cctctttcat taaacaaaat aatcaggttc tgcaagaggg cctaatgaat tccaatttat
     8461 cctatcatgg gttgaaattt atcagtgacg tccccggaca aatgttgata aacattcagg
     8521 gcgattggtt gataaagata atcaaagtca atgctatcca ttggaatcac tcttaatgca
     8581 gcctcgttaa tgctgcggaa gtaaattgtt ttggtcggga tatcaaaaac catactcagt
     8641 tgggttggag aagaagtgcc aggtgcttgt gtgacgacat tgaggccgtt gaaagcataa
     8701 gcaatatgtt cttctttagc tataaagctg ggaagttttt ttagataatg tgctgctctt
     8761 acaaaacggg atttagaatc atatccacct ggcagagcct tggagccacc atggtcctgg
     8821 tattcttcaa gaagagccaa tgacgattta tagtccgtat tggttagtac tggcgtggtc
     8881 aagttgtctt gagtatgaat taccagttta ccatccaggt actcaaaaac agcagatttt
     8941 ccttcggcat cgctcaccat caagtgaacg ttcatggtta ttccccggta ggtgtctgca
     9001 accagttgat aatttgagct gtcatctatg acttcttgga ccgtttgaaa attatctaaa
     9061 atatactgaa cccattcagt agtttttaaa gcgggaagct cagagttact gggataactt
     9121 gatgaatcaa gcatcagtat tgaagcagta agtccttttt cattcatccc atcagcagat
     9181 ggtcctgctt taaaccgatt tcctccgtga aatatcacac ttccatattg gcttttccag
     9241 attaatggat gcatgctgtg gtaatctaca ctggaaacca tcatggcatt tcgaggatga
     9301 atcacaaaag ctccttcccg gtatttccag tccaaattga ctgcaacagt tgcgggcttg
     9361 tttttattga acactaaggc agaacaggct tcgctggtta aggagatagt atttaaagat
     9421 aaggcaatcg atattagtcc tatgcgttta aacatttcaa atccttttgc ttattatatt
     9481 cttggaacag gtttgcttac ccgttcaacc ctaaaacatg gacagcaaga tatcttaccg
     9541 catagtacat atacttgtca ttttttgtga aaatgccggt attttgacct ttataaaaat
     9601 gaaatacagc acaagttcta attccaataa agtaacaatt taagtagaat gtttactatt
     9661 ttatgaattt ggggcggtta tgactgttaa aagacatact atagatttgg cacatcttgg
     9721 catgcgcgat ttggatcagg tcggtggtaa gaactcttct cttggtgaaa tgattagtca
     9781 tttgtcatca gcaggcgttt ctgtgccggg aggatttgct acaactgctg attctttcag
     9841 agaatttttg tcccaaaacg gtcttgataa gaaaatttat gataagttga ctgctctcga
     9901 tacggatgat gtctgccaac tggcagtggt agggaaacaa attagggaaa tgattattga
     9961 tacgccattc accccggagt ttgaacagtc ggttcgctct tcctaccaga aacttgctca
    10021 agagattggg catgataatt ttagtgttgc agtaagatct tctgctactg ctgaagattt
    10081 gcctgatgct tcttttgccg gtcagcagga aactttttta aatgtgaagg gagaagaggc
    10141 ggtattggct gccattaaac aggtatttgc ttctttattt aatgacaggg caatagctta
    10201 tcgtgtgcat cataattttg ctcataacga agtggcattg tctgctggca ttcagcaaat
    10261 gattcgtagt gatttggcag tcagtggtgt catgtttact atggatactg aatccggttt
    10321 tgatcaagta gtatttatca cttcctctta tggtttaggt gaaatggttg ttcaaggcgc
    10381 tgtaaatcca gatgaatttt atgtccataa accatgcctt gaagccggaa aaccggcgat
    10441 tatccgtcga aacctgggca gcaaggcgtt aaagatggtt tattgcgatg acccttccct
    10501 tgaaagacgt gttaaaacag tcgatgtaga ccctgctgaa agattattgt tttccttatc
    10561 caaagaggaa gtagagcaat tagcgaatca ggcacttatt attgaaaaac attatggtcg
    10621 ccctatggat attgaatggg cgaaagacgg ggtcaatggt aaactgtata tcctgcaagc
    10681 tcgtccggag actgtaaaaa gcagggataa caaacaagtg ctcgaacggt atactttgca
    10741 aaaaagaggc gacgtattgg ctgaaggccg tagtataggt caaagaatcg gtcagggtaa
    10801 agcaaaggtt attaaagata ttaatgaaat gcacagggtt caacccggcg atgttttgat
    10861 atccgatatg accgatcctg attgggaacc tgtcatgaag cgtgcttctg caatcgttac
    10921 caatcgtgga ggcagaactt gtcatgcagc tattattgct cgtgaattgg ggattcctgc
    10981 agttgttggg tgtggtgatg caacaaaaac gattaaagat ggtgatgtgg tgactgtaag
    11041 ctgtgcggaa ggcgatacag gttttgtcta ttctggcctg ttgccttacg agcaagagcg
    11101 tcttgatgta gaaaccatgc ccgaattacc catgaaagtg atgttgaatg ttggaaaccc
    11161 tgaaagagcc ttcgcatttc aatcaatacc taattccgga gtgggattgg cccgtcttga
    11221 atttttgatt tccaacacga taggtattca tcctaaagcg cttctggaat ttgatacttt
    11281 aaaggatgag gaacttaagc ggtacattaa agaaaagacc atagcgtatg attcacctgt
    11341 agaatattac attgaaagat taaaagaagg catagcgact attgctgctg ctttttatcc
    11401 caaacccgtt atagtcaggc tttcagattt taaatcgaat gaatacgcta atctggttgg
    11461 tggctcattg tatgaacccc aagaagaaaa tcctatgctt ggttttcgtg gcgcctctcg
    11521 ttatgtttct tcaaattttt ctgagtgctt tgctcttgaa tgtaaagcag taagacgagt
    11581 cagggaggag atgggtttag ataatgttga ggtcatgatt ccttttgtcc gaacagtttc
    11641 tgaagccggt aatgtcatcg aagtattaaa aaagcatggt ctggaaagag gaaaatgtgg
    11701 gttaagagtg attatgatgt gcgagttacc ttccaatgcc ttgttagcca gtgaattttt
    11761 gcagtatttt gatggttttt ccataggatc gaatgattta actcaattga ctttaggatt
    11821 agacagggat tcaggtttgg tggcttcaca atttgatgag cgcaacgatg ctgtgaaagc
    11881 tttattgcat atggctattg cagcttgtaa aaaagaaggc aagtatatag gtatttgcgg
    11941 tcaaggtcct tcagatcacc aggattttgc ccaatggctg atgaaggaag gaattgagag
    12001 tgtttctcta aaccctgact ctgttttaca aacatgtttg tttttagcta aacaatcgat
    12061 gaattgatga accagagtgt actggcaatt cagtttgaaa ttgttaggct gttggtgcat
    12121 taggaattta ctgaggcatt cctatgattt tgaaatgcag cagattgcct tggaagatga
    12181 gttttttgat caatacaatt attttggctg ttagtaatgg aactacttgt gatggtatgg
    12241 tgttgcaatt caaaattccc ttgcatttgg gagaaagcag cacattaagc ttataggata
    12301 aagcggtatg agtaattcaa aatggtggtt tcttttagtc tgtttcattc ctgctattac
    12361 ttttgcatct gaggggggag agcattcaga tccagtcacc tctgttcttc tgggtgtcac
    12421 tactatttta tttttagcta tcattggtcg ttatttagcc agacttctta atcaacctgg
    12481 cgttcttggt gaattactga tagggatgat agttgggaat ctctcttact ttttcggaag
    12541 tgaattagta gtaattcttc gcgaaggttc tgccatttat gatgttgtca aagaaatgtt
    12601 agcaggaatt tctttaaatc aggcggtaac tcatgttatc cataattcct attatgctaa
    12661 ccaggtgatc catgccttaa gtggagcaga gggaactgaa tatcttaaaa tagcctatgt
    12721 cgttgatatt ttctcacgtt atggtgtcat atttcttctt tttatggttg gtcttgagac
    12781 gtcgctttct gatttgaaaa aaactggcaa agaatccatt cttgttgctg tgatcggtat
    12841 tgttgcgcca atgattttag ggtttgtagt ggcttgtttt atcataccta actcaaatta
    12901 caagattaat ttatttatcg gcgctacctt gagtgctacc agcattggta ttacagcaag
    12961 tgttcttaaa gagatgaata aactgagtac acgggaagca agaacaattc ttggtgcggc
    13021 tactttggat gatattttag gcctgattat tttggcggta gtcagcagta tcgttatcag
    13081 tggtgctgta agtatcatgg ttgtaatgaa aattatcatt ttagccctac tgtttttcat
    13141 tggtaccttg tttatggggc cttttgtact tcggaaggca gtcgatttat tccgttttct
    13201 ggagccttgg gaatccaaat tatttatctc attccttttt attatggcat tagcctggtt
    13261 ggcttcattt atacagttgg cggctataat tggtgctttt gctgcgggcg tcattttgca
    13321 cgacgactat tttggagagc aaaaagccca tcataaagat catcgcagta ttcatcattt
    13381 ggtatctccc ttggagacta tattggctcc tatgtttttt actctcattg ggatacaggt
    13441 taagttagaa actttttatg attggaatgt cattgtttta gcgggaggct tatcagtagc
    13501 ggccattctt ggcaagctgc ttagtggctt aggcgctaat cggcgggatg ataggctgct
    13561 cattggtata ggaatgctgc ctcgtggaga ggtaggtttg gtttttgctt ctattggcag
    13621 aactataggc gtaataagtg ataatctgtt taccgcaatt gtattgatga tcatgatcac
    13681 aacctttatt gcgccacctt tattaaagat gcgatatgcc aaaccaaaag aggataggaa
    13741 tgcatgaata atgttttatc gcaagttaca tttgatgtaa aacgtgcttt gcaagaggac
    13801 gtgggcaatg gtgatgttac tgcggctttg ttgcctcagc agctgattgt agaagcagaa
    13861 attatatcaa gagaacctat gcttgtttgt gggcagccct gggtaaatga ggtttttaag
    13921 caagttgata atacagttga aatggagtgg cgggtttctg aaggagattt tttaagcacg
    13981 ccagctactt tatgtattat tcatggtact gccagtagta ttttaacggc agagcgtact
    14041 gcgttaaatt ttttgcaaac cctttcagct acggcaacac aaacttatca ctatgttcag
    14101 aaattaaaag gtaccaaggc aaagctgtta gacacacgca aaacaattcc aggacttcgg
    14161 ttagcgcaaa aatacgctgt ccattgtggt ggcgggttaa atcatcgtat gggactttat
    14221 gatgcgtttc taatcaaaga aaatcatatc aaagcctgtg gttcagttgc tcatgctatc
    14281 agtctggcaa ggaaaaccga taaacactta ctggttgaga ttgaagtgga aacccttgag
    14341 gagttgcaag aggctttgga tgctcaccct gacaggatat tattggataa tttcactcaa
    14401 gatatgcttg agcaagcagt caaaatgaat catcccaagt actgtgaatt ggaagcctct
    14461 ggcggcatta atatcaataa tattgcagac attgctcaaa ccggagtgga ttttatttca
    14521 gttggatcca tcaccaaatc aattcaagca attgatttga gcttattgat tagagaaaca
    14581 ttatgagccc aagtattgtt tttaccgggg gaggcactgc cggacatgta acgcctaata
    14641 tcgctttgat taaggaattt cgaaaagaag gctggaatgt agaatatatc ggctctgttt
    14701 ccggaattga aaaggagatg attgagccga tggacattcc ttttcatgga gtcagtagcg
    14761 gtaaattgcg caggtatttt agtttgaaga acttgcttga tcctttcaaa attgttctgg
    14821 gaattattca atcttctttg ctattttata aaatcaaacc cgatgtggtt ttttcaaaag
    14881 gtggctttgt agcctttcct gtggttgtag gcgcctggtt aaatcgaatt cctgttgtcg
    14941 ctcatgagtc tgatatgagt ccaggacttg cgaatcgcct atcctttcct ttcgtcaata
    15001 aaatatgcct tacttttgat gctggcaaaa aatactttaa gcgtcaggat aaaatagaag
    15061 tgacgggtac tccaattcgt caacagctat taactggaaa tcgaatgaaa ggattggagc
    15121 tatgcggatt taattcctcc aaaccttgcc tgcttgtagt gggaggaagc ttaggggctg
    15181 gttcaattaa cagttgtatt cgaagcgcat tgaaacaatt gacatcagaa tttcaagtca
    15241 ttcatctttg tggcaaggga aaacttgatt cttcattagt tggtgtggag ggatattgcc
    15301 aatttgaata cgccaatgaa gagttggctg atctgttcgc tgcttcttct gtggtgattt
    15361 ctcgagcagg agctaattct ttgtatgaaa tattagcatt aggaaaacca catatcttaa
    15421 ttccaatctc ttcacaagta agcagaggag atcaaattca gaatgcaagg tacttccagg
    15481 gattgggaat aagcgttgtg attcaggacg agttattgaa agctgatgtt ctattacagg
    15541 cagtacagga cgtaatgcga aaaaaagatg aaatagataa taaaatcaaa gcattaaaaa
    15601 ttgagtctgc cactgataag attgtggcaa ttatcaagga gcaagcacat gttcaaaccc
    15661 caaggattgt atgattacat atgccaacag tggcaagaag agatattgcc aagtttatgt
    15721 gactatataa aaatccctaa taaatctcct cactttgatg caaaatggga agaacatggt
    15781 tatatggagc aggcagttaa tcacattgcc aattggtgta agtcgcatgc tcccaaagga
    15841 atgactctgg aaattgttcg cctgaaaaat aggactccat tactatttat ggaaattcca
    15901 ggccaaattg atgacactgt gttgctttat gggcacttgg ataagcaacc tgagatgtca
    15961 ggctggagtg acgatttaca tccatggaaa cccgtattga aaaatggatt gttatacgga
    16021 agaggaggag cagatgatgg atattctgct tatgcatcac tcacggctat tcgcgccttg
    16081 gaacagcaag gtttgccata tcctcgttgt atattaatca tcgaagcgtg tgaggaaagt
    16141 ggcagttacg atttgccttt ttatattgag ttgctgaaag agcgtattgg taaaccatca
    16201 ttggttattt gtcttgattc cggagcaggt aattatgagc agttatggat gactacgtca
    16261 ttacgcggta atttagtcgg taagttaact gttgaattaa ttaatgaggg cgttcattcg
    16321 gggagcgcca gtggtatagt ggcagacagt ttcagagtag ctcggcaatt gatcagcagg
    16381 atagaggacg aaaacaccgg agagataaaa ttacctcagt tgtattgtga tattcctgat
    16441 gagagaataa aacaagcgaa acaatgtgcg gaaattctag gtgaacaagt ttatagcgaa
    16501 tttccatgga tagattctgc caaacccgtt attcaagaca aacagcaatt aatattaaac
    16561 agaacatggc gacctgcctt gacggtgact ggtgcagatg ggtttccagc gatagctgat
    16621 gcagggaacg taatgcgccc tgttacgtct ttgaaattat ccatgcgtct tccaccactg
    16681 gttgatccag aagcagcttc tgttgctatg gaaaaagccc tgacccaaaa ccctccctat
    16741 aatgcaaagg ttgattttaa aatacaaaat ggaggttcca agggatggaa tgctcctttg
    16801 ctttccgatt ggttagcgaa agcggcatct gaagcatcaa tgacttatta tgataaacct
    16861 gctgcctaca tgggagaagg gggaaccatt ccatttatga gtatgctagg cgagcaattt
    16921 cccaaagcac aatttatgat aactggtgtt ttaggccccc attccaatgc tcatggtccg
    16981 aacgagttct tacatttgga catggtaaaa aaactcacct catgtgtctc gtacgttctt
    17041 tatagttttt cacagaaaaa ataatttgaa attggctaga ctcttgtttg aaaaattgag
    17101 atactcgctt gcggctctgg aacgagccgt ttagtccaac aattaggaga aatttatgaa
    17161 agcaagactc attgctattt tgttgtcatt tattgttatc cctttctctt ctcaagctga
    17221 gaacgagcct caaaaaactc cagctaaaca agtccttgtt aagggcaaga ttaatctcaa
    17281 tacagcggat gtgcattctt taacaggctc ttttaaaggg ataggaaaaa aacgggctga
    17341 agctattatt gcttatagag agaatcatca aggctttaaa tctctggaag agcttgcgga
    17401 agtcaagggt atagggcagc gttttgtaga taaaaataga gaaaaactga aagaaatttt
    17461 cgttattaat taattcacgt gcaatataaa acgtaaatca tgttttataa agcaaatgag
    17521 attgatccca tatgaaagcg ttgcttttac tatgggatcc ggttaaaaga agttgtttgg
    17581 tgtataaaat tcttatgcgg caactgtcga tttttatcca aatataggat aaagtagcaa
    17641 tcaaaaaaac aacggggtag tactgcgtat gttcaggaaa ttaagaggcg tgttttctag
    17701 cgatttatca atcgatttgg gaacagctaa tacattgatt tacgtaagag ataaggggat
    17761 tgttctcaat gaaccttctg tagtggcatt gcgtaatgaa tcaggccaga agcgtgtcgc
    17821 cgctgtaggt cttgaagcca aacgcatgtt gggaagaacc cctggcaata ttaatgcgat
    17881 aagacccatg aaagatgggg ttattgctga cttttttgtc accgagaaaa tgttgcagca
    17941 ctttatacac aaagtgcatg aaaataaatt tttacgaccc agtccacgcg ttttggtctg
    18001 tgttccttgt ggttctacac aagtggaacg cagagcaatt cgtgaatctg caatgggcgc
    18061 tggtgctcga gaagttttcc ttattgaaga gcctatggct gcggcattag gttccggaat
    18121 gcctgttgag gaagccagtg gttctatggt ggttgatata ggcggtggta ccacagaagt
    18181 cgcaattatt tcactaagtg gaatcgttta tcatcaatca gtacgtattg gtggcgataa
    18241 atttgatgac gcaatcgtgt cctacgtacg ccgtaattat ggtactctca tcggagaaac
    18301 aactgctgaa cgtattaaac atgaaattgg ttcggctttc ccaagccggg atttatttga
    18361 gatcgaggta cgaggtagaa atcttgcaga aggagtacca cgtagcttta ctttaacaag
    18421 cgctgaaatc ctggaagcac tacaggaacc tctatcaggg atagttggtg cggttcgagc
    18481 tgctttggag cttgctcctc ctgagttggc tgcagatatt gctgaaagag gtatggtttt
    18541 aacaggcgga ggcgctctgt tgaaaaatat agacaccttg ttgatggaag aaactggatt
    18601 acccgtatta atagctgaag atccattaac ctgcgttgcg cgtggtggtg gtaaagcttt
    18661 agaaaccatg gatttgcgcg gcggtgattt cctctcaaca gaataataaa cacaatagat
    18721 tatttgctaa gggcggacac agttctctgg gatttgtgtt tgcccttgct ttttctattc
    18781 tcctgatgtt ttctgattat cattatcgat acctggattt cgttcgtagc ggtttttcac
    18841 taatagtttc tcccttgcaa tatgcggtag attatcctgt tcgagtcatc ggctggattc
    18901 agtcgttggt tagtgccaaa aaggcactga tagatgaaaa tatgcgttta aaatacaagc
    18961 aaacaatgct tgaagctgaa ttgcaaaaat taattgttat ccaaagagag aattcgcaat
    19021 taaaagaatt attattgact tcatccaagg ctgatatgag agtcatggct gcccagatac
    19081 tcgctgttga tacaagtcag gcaagacaag ttgtcgtctt aaacaaaggc acacgtgatg
    19141 gcgtctatgt gggacagcca gtgttggatg ccaagggagt gatggggcag gttattgatg
    19201 taggaccaat gacaagcact ttattactga tttcagattc aaaaagtgct gtgccagtaa
    19261 gaaacaatcg aaccggagaa cgcgcaatcc ttgtaggcac taatgatatt gaagaattgt
    19321 ctttgattaa tttaccgaag acttcttcta ttcatccagg tgatgtgctg gtaacgtccg
    19381 gtttaggccg aagatatccg gagggctatc ctgtaggacg cgttgaggaa gttaagagta
    19441 ttcctggcga agattttgtc aaagtcacag ttagccctgt cgcattgttg aatcgcaaca
    19501 ggttggtgtt attgatttgg gctgatagtg agcaagagga attaactgct caaattaatg
    19561 agcgattgaa tgctgaggag tctacagcat gagcgtgctt cacattcgtt taggattggg
    19621 gtttctcgtg gcacttgctt tatcaatact gccgatgcct gagttaattt caatttttcg
    19681 acctcc
//
LOCUS       DATNSN010000047        19311 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.047, whole genome shotgun sequence.
ACCESSION   DATNSN010000047 DATNSN010000000
VERSION     DATNSN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19311
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.047"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            127..1341
                     /locus_tag="VAH76_001381"
     CDS             127..1341
                     /locus_tag="VAH76_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386016.1"
                     /translation="MSHLLDLSILKKNRDFTLLYLGQFISFIGTMITSVALPYQIYHL
                     TESTMMVGLLSLAQLLPLLITALLGGVFADRYSRRGLLIISELLLAVGCLFLAFNSKL
                     VNPSVSLIFIIASLMSAITGLHRPAFDSVIQQIVKPEDYKKVGALGSFKFSFCMIIGP
                     AAAGLIIAQYGIVITYLIDLLTFIISLLNLSLMHTIPKPLAKEHPSIIVSLKEGIGFA
                     LRRQELMGSYFVDFFAMVFAMPNALLPAIAQQFGGAKTLGLLYAAPAIGSLIISFVSG
                     WTAKITHDGKAIAIAAALWGVAIIGFGITSSLWLSLLFLALAGAFDAISGIFRSSLWN
                     HTIPQELRGRLSGIEMISYLSGPRLGDTRAGFVAAGLGIPAALISGGILCIIGVTVCC
                     YALPKFWNYHSK"
     gene            complement(1354..2670)
                     /locus_tag="VAH76_001382"
     CDS             complement(1354..2670)
                     /locus_tag="VAH76_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1639 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386017.1"
                     /translation="MAGIYLKYYVPVNQIGENMMINPIEYHPKGDKVNSDYFNEYKTK
                     IYERRLSSGLEDLFGDMCGVVVQVQTGDAIPYIKELYSMTPYRYSRSYISDTHKIYCL
                     LNTKNSPAFLVLEPLDPNFKDDITRLNKMYPNSRAKFNARYVGEIYKCKDKDETRNIL
                     VSHDFNFHNPDMTENKFYCNKHIHFTRLSDFTYNCTGYTDDNIYDFDCLELGQRFYLT
                     KEQEAMLDAKDQESHDNGIKDLIKGIDHMATRILAGEREDAILEFLCLSKYYFWGAYN
                     IYDMNSSTNVNRNPHGHDIKSPAKVFTANNTPFMVNSFENLPMPTETFVRNYGRRMHH
                     IAYEVKDGDHSSGKKNIDFVVETLRDKLNIEFLAKVFGACEDSPDLKQIFSKHSIYSI
                     LITEYVERCHNFDGFFTKENVAALTEAASLDETTKQQHKKASIIGD"
     gene            2761..3318
                     /locus_tag="VAH76_001383"
     CDS             2761..3318
                     /locus_tag="VAH76_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEO1386018.1"
                     /translation="MEALIVSLGVITLAEIGDKTQLLAFLLAAQFKKPLPIILGILTA
                     TLINHSLAGLIGIWITTLLKPNVLRWILGISFILMAIWTLIPDEIEQDEKKISKYLGV
                     FGATFITFFLSETGDKTQIATIALAAHYSSPILVIAGSTLGMLLADLPAVYFGNLFSH
                     KIPMKIIHAVAACAFLIIGLITILF"
     gene            complement(3336..5306)
                     /locus_tag="VAH76_001384"
     CDS             complement(3336..5306)
                     /locus_tag="VAH76_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEO1386019.1"
                     /translation="MKGVCLGLIICLVVFHDGWAGKRLPELNDLYRIQDISEPDLSPD
                     GKWVAYTVSQLNAKDNISTTEVWKVSVDSKSSQRITFAEDSSSSLPKWSPNGKWLAYL
                     SDGGKEGTTQIWIASVQDNKEHQLTHIDGEVSDFTWSPDSNRIAFIAQKSDPSNEEEQ
                     PIVVDRYQFKVDGVGYLGKGRDHLYLVELKSKAITQLTDGNHDEYLPAWSPDGKYIAF
                     VTKRGKDPDRHFNYDIYLIEPKSGAKEHQLTHFPGSDMNPDYDSNLSWSPDSSKIAYL
                     RSNEHQWTYYSPNQLAIIDINTKDEKIIAPRDNWYYKPKWSQDGKFIYALIEESRNTY
                     LNRIDIHSGQIKKLTGGLRYDMDYSLAKGKIILLTTDDTHPSELFILNSGLIPLTQHN
                     QKLLDEVKFQPAEDIQCNSFDGTRVDGLLMKPAEYQPGILFPGFVYLHGGPVYQFSHE
                     FNFDMQWIAANNYAVIAPNPRGSSGRGFHYAKAIFADWGNLDVKDILASVDYVIGKGM
                     VDPNRLGIGGWSYGGMLTNYVIATDSRFKAAISGAGAANILAGYGVDQYTPEYELELG
                     KPWTNPELYLKLSYPFLKANNIKTPTLFLCSGLDFNVPCVGSEQLYQALKSLDVPTQL
                     VIYPNEYHTLEKPSFVIDRLKRYTNWLDTYVK"
     gene            complement(5516..6769)
                     /gene="sidB"
                     /locus_tag="VAH76_001385"
     CDS             complement(5516..6769)
                     /gene="sidB"
                     /locus_tag="VAH76_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidB"
                     /protein_id="HEO1386020.1"
                     /translation="MAKIYNAPKPKYSGWEWFKFIAIRTVFPPVLLWDLIKNGANKLL
                     GEWVSGLVLPAQNENFDDLAVSDDTVSNYNEDGLICEKHDVITHDGAHLDTFEVRHRS
                     QESIDPKYQKYIINLVGNGMCYEHIIDDIKEDSKALKANVIGFNLRGVGQSTGKAKSS
                     EDLVADGIAQVQRLLDQGVSPQNITLKGHSLGAGVASLVAQHFHQLGQPINLFNSRSF
                     STITNFLVGHMRLERDEIGRAIGHKDSTVGTILGWLAKPFIKFGVALAKWEINAGSAF
                     KSVPEAYKDYIVVRSRKEIRGERIDDAVIPHYASIHKELTSERQKKKAEIDEEIANLD
                     DIIRKADPLAKPGLATARDALVQAREKIKSDRKMETDVQYANGHNSDWNALHNRSGKS
                     AQTFFREFVQRTEADHAVKSIPEIN"
     gene            complement(7148..8416)
                     /gene="thrC"
                     /locus_tag="VAH76_001386"
     CDS             complement(7148..8416)
                     /gene="thrC"
                     /locus_tag="VAH76_001386"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO1386021.1"
                     /translation="MKIRIKSTRGQYVSTIDEAILSGLAGDGGVFVPDRFPNLTIKKF
                     YEYGNLIDFSVNLLSPFFSDSKIDVNNAFFQKFLTFSFPLHHLFKKSYVLELFHGPTL
                     SFKDLGTQFFSECLLHLIENKSAKVMVATSGDTGAAIAHALHGKRDLEGIILFPKDKL
                     TFRQQSQITCWGENIRALAVNGSFDQCQQLVKLVFAKREHKGVLTTANSMNIARLLPQ
                     IIFYAFTSIQLALRHNHSVNFIVPGGNLGNVTACYWAKMLGFPIEQILIANNTNSALG
                     EFLLTGAYQAKSVTKTLATAMDVGEPSNLERLFILFNNYSECRKQMNAESVSDEQIKQ
                     AILHCYNKYHYIICPHAATAYHRLSTINQDKPWVIVAPGHPAKFEEVLEPLLNKEIPV
                     PEQLKVFLGRKQHYSIIEPNYHSLYHFLVE"
     gene            complement(8588..9352)
                     /locus_tag="VAH76_001387"
     CDS             complement(8588..9352)
                     /locus_tag="VAH76_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1386022.1"
                     /translation="MYLGFPVQSILLFLLFSFTPLFVLAAEKTQLNFFLRPDTYFGFS
                     VIRDTAHYNFKRNETSQELNFTDFDHFDWHGSGLGGEIYLGHEKFFRTHYYFGLEGFF
                     NRSSNEGKIYFFDSNELYSRILKGSFKQKWQSGLAIHPGIYLPSVGIIYGRVGWIISD
                     VNLSGSITQSGPLGSFSGRFSNTKKKNGVQLGTGLELELTQNWRCRVEWDWNRLQDFT
                     FDTQGKDSNNHRYQTTRIAKHPILEQFKLGLNWRFS"
     gene            complement(9653..11677)
                     /locus_tag="VAH76_001388"
     CDS             complement(9653..11677)
                     /locus_tag="VAH76_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1386023.1"
                     /translation="MPIPLLNFKHFQNDNSIFRSTTHHNHMVSRGTFPGNPKTWFAKV
                     CSDPASARNEVLAQEFFRLIIPHQPETLIAKDEAQNNYYVYSEEVEGFKNLPYGQGKS
                     FEDGTFTGLGQAVFVSVFMQEVDLKNGNIGLDKDGRVIKIDGDQCIVSMLGVDQRFDL
                     TPKVIDSLPRPVDFATNNWLDYKSSFFSLQRILQPTITNNEVFRSEVNQAMLKICLIP
                     DEYIERFVSAYIPDGHKPDKQGYIDLIKSRRDVLRLSAVKNESFRNYLSEPAAQEDAK
                     HFMEHMRSFKSGGEPVLASQQERSQLTIDLALQNLKLETAFIQQSEKVATVKNAVEKE
                     AFADQENELAMVFRFQMEMKKQAQLSDSSFISIISRSSQASAEKTAYQNFLNKSVNPV
                     MEKCFAALVRNDLKQLEQVLAEFPNNEKWSAFNLQESEVAKAQMDAIKRLIEERVFIR
                     GKILPVLEQCKDAIDKKDIPAALQALSAFPPDKDMSKTTSISSGLKGQIIEMKQDAIE
                     NLNAIAQQSAISTPVIDDAEKVESGYSPLLVEITKQIEALESEHCEDLPKLEMHLTTV
                     NDCLDTVEFLISERIKQHGASEDPLELSSLEELRSRLQKIKKTDENALNAYPKDNAEI
                     TQTETQPSQVLPQTSNNTNPAKRTIKSDYDVDSENKVTKIGEDRPGFKPE"
     gene            complement(11824..12399)
                     /locus_tag="VAH76_001389"
     CDS             complement(11824..12399)
                     /locus_tag="VAH76_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEO1386024.1"
                     /translation="MNVYIKSSLWVLIFFVNTLTYASKLPEWEIVPAQSEITFTGTQN
                     GAPVTGRFKKFTGQIFADPSNYKTGSIDITIDMNSIAAPFEDIVTTLASPDWFNVKTF
                     PNAEFKASKFNKLDDKTYEAEGILTIRDKSAPVTLKFVVDQISNDQALVEGSSTIKRS
                     TFGVGQGEWESTDEIQDEVIVRFKITATRKK"
     gene            complement(12399..12929)
                     /locus_tag="VAH76_001390"
     CDS             complement(12399..12929)
                     /locus_tag="VAH76_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEO1386025.1"
                     /translation="MQLKNTSTSFGLVAIFFHWIMAALIIGLLGVGLYMVRIPISLEK
                     LKLYGWHKEYGFLVLFLAFFRLIWRLTNQLPELAIPLLEKIAARSMHWAFYFFMFAMP
                     FSGWLITSAAGLPASFFGLFVLPDLVAPDENRRILFQLIHEWLGYALIMAIILHAAAA
                     LKHHFINKDDILRRML"
     gene            complement(12933..13526)
                     /locus_tag="VAH76_001391"
     CDS             complement(12933..13526)
                     /locus_tag="VAH76_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEO1386026.1"
                     /translation="MKRIYRHFIASILAVFALTSYVYAEPQTLTLDNQHTYVLWKVKH
                     LGFSTQAGKWYASGQLVLDKDNPQQSKVNVTIKVDDIVTGIPELDKHLKGKLFFDTKQ
                     FPTATFVSNRVEVMGENKAKVYGMLTLHGVTKPIILNVILNKAGINLLNDRETAGFSA
                     TTSLKRSDYGIKALIPEVGDDVEIEIEAEAYLDKKYG"
     gene            complement(13554..13811)
                     /locus_tag="VAH76_001392"
     CDS             complement(13554..13811)
                     /locus_tag="VAH76_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEO1386027.1"
                     /translation="MSMKDKLIQSAISAFLVLIASNTAIAEDLSKEKCYGVVKKGMND
                     CSTATSHCAGSSTRDSQKDAFILLPKGICEKLVGGSLDMGK"
     gene            complement(13933..14829)
                     /gene="iolE"
                     /locus_tag="VAH76_001393"
     CDS             complement(13933..14829)
                     /gene="iolE"
                     /locus_tag="VAH76_001393"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEO1386028.1"
                     /translation="MEVKMSIQLGIAPINWCNDDDPNLGKDISFEQCINEMALAGYSG
                     TELGNKYPRDATTLKAALEQRGLQLSSSWFSTYFTEANAFEKTVSRFLEHLSFMRALG
                     ASYINVCECGHAIQGTDLPILSAHIPQFTQEQWSLLIQGLHTLGRIAYDFNMKIVYHY
                     HAGTGVFWEKDIDFLMTNTSPKLLSLLLDTGHAVFSGIDPVNLINKYGDRILYVHLKD
                     IRPEVLRRVEMERLSFMDAVRRGVFTVPGDGAIDFIPIWAGLQKHRYNGWMIVEAEQD
                     PEKAHPLEYAKKAYDYLHSFIH"
     gene            complement(14832..16703)
                     /gene="iolD"
                     /locus_tag="VAH76_001394"
     CDS             complement(14832..16703)
                     /gene="iolD"
                     /locus_tag="VAH76_001394"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEO1386029.1"
                     /translation="MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTG
                     LGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAA
                     TATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
                     LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAML
                     AEAIRHIRKAKQPLIIAGGGVHYSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMN
                     VGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAM
                     KMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQA
                     GLHQTAVLGLLNQYVTEEDVIISAAGSLPGDLHRIWQSKRAKDYHLEYAYSCMGYEVA
                     AGLGVRIAKENQEGEVYVLVGDGSFIMMHSELLTAIQERKKITILIFDNHGFQCIRNL
                     QEGNGSLGFGNEFRYREPTTNALSGDYLPIDFCKYAEGLGAATYFVKSYEQFRTALDS
                     AKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMQEQIETVRQY"
     gene            complement(16742..18628)
                     /gene="iolC"
                     /locus_tag="VAH76_001395"
     CDS             complement(16742..18628)
                     /gene="iolC"
                     /locus_tag="VAH76_001395"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEO1386030.1"
                     /translation="MKQFPGFYFDKNRAIDLICMGRVAVDLYAEQIGLDLKDVASFKK
                     YLGGCAGNIAVGAARLGLKSQMFSCVGSDELGKFLKEELEREGVNIELLSETDNHLTG
                     LVLLGIKPPSNFPLLFYRNDCADMQIKSDQISFTDLKNAKALLITGTGLSTESMKNTT
                     LEVVHLARKAETTIVFDLDYRPVLWRLTVIGNGESRYCQNEYVTQVYQQVLPLCDLIV
                     GTEEEICIAGGSNDIEVSLINIRQRTDAPIVVKLGEKGARVHFGTDKGLLQAKSFPVE
                     VLNVLGAGDGFMSGLLSGLLQGKSWEQAVTYANACGALVVTRHGCAPAIPYKEELDYF
                     IQEYEHDPEIWSSSQLTQLHEKLSKNQKTQLLIKNPCGFADGLNSIVTMQDSPTAMSF
                     SSLKLNKGQSHQFNSSYEFAALLMTGKVVFQYGSYSQEVERTDYFSQSPYVLHCSKNE
                     VSSVIALTDCEILLMETENENLFSPILFDASNMLELDNRGEGVLENTSYRLVRTVFDK
                     RNRPESNLVVGEIITFQGRWSSYPSHYHQQPEIYHYRFSEPQGYAFGENGKEVMRIEH
                     YDTYQIANNQEHAHCAAPGYALYTLWFIRHLNNNAYTLPTFIKDHEWTRTSRANLRAW
                     QQKQ"
     gene            complement(18636..>19311)
                     /locus_tag="VAH76_001396"
     CDS             complement(18636..>19311)
                     /locus_tag="VAH76_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEO1386031.1"
                     /translation="NIIKNNNTLLQLGFNRRFDPSFASIQKRVQFGEIGTPHILRITS
                     RDPACPSKEYCATSGGIFMDMSIHDFDMARFLMQSEVVEVYATGAVLINPDFEQFDDV
                     DTAIIQLRFANGAFGVIDNSRQAIYGYDQRIEVFGSAGMLLAENQLEHTVSRYSANGT
                     DGAKPQYFFLERYHQAYLAELNAFYTAWNDNQTSPVSGLDGLQALRIAKAAKQSLETH
                     LPVVLS"
BASE COUNT         5810 a         4026 c         3212 g         6263 t
ORIGIN      
        1 tgaggttttt cgtaatgctc ttatagtctg atcttattaa tttctgtact gtttcatcaa
       61 aagagcttaa aattcatatc tcaaaatctt tataattcac cccactataa tttacagatt
      121 gccaatatga gtcatttgct agatttatcc atccttaaaa aaaatcgcga ctttaccttg
      181 ctttatttgg gccaattcat ttcattcata ggcacaatga ttaccagtgt cgcccttccc
      241 tatcaaattt atcatttgac cgaatcgacc atgatggtag gattactcag cttggcacaa
      301 ttacttcctc tactgattac tgcattgctt ggtggagtgt ttgctgatcg atatagccga
      361 cgcggattac taatcattag tgaattatta cttgctgttg gttgtctttt tctggctttc
      421 aattccaaac tggttaatcc aagtgttagc ttgatattta taatagcctc attaatgtcc
      481 gccattacag gtttacatag gcctgcgttt gatagtgtga tacaacaaat cgtgaaacca
      541 gaggattaca agaaagtagg cgctcttggc agcttcaaat tcagtttttg tatgattatt
      601 ggaccagctg ctgctggcct gattattgcg caatatggta ttgtaatcac ttatctcatt
      661 gatttattaa cctttatcat ttcacttctc aatttgagct taatgcacac catacccaag
      721 cctttagcca aagagcatcc ctccattata gtttctttga aagagggtat aggttttgct
      781 ttgcgccgac aagagctgat gggtagttat ttcgttgatt tctttgcgat ggtttttgct
      841 atgccaaatg cattgttgcc cgctattgcc caacaatttg gaggagctaa aacactagga
      901 ctactttatg ccgcaccagc tatagggtcg ctgattattt cttttgttag tggctggacc
      961 gcaaaaatta ctcatgatgg aaaagcgatc gcaattgctg cggctttgtg gggagtggca
     1021 attatcggat ttggtatcac cagttctttg tggttatctc tcttgtttct tgctcttgcg
     1081 ggagcatttg atgccattag tggtattttc agaagcagct tatggaatca taccatcccg
     1141 caagaattga gaggccgcct ctctggaatt gaaatgatta gttatttgag cggccctaga
     1201 ttgggtgata ccagagcagg ctttgttgca gcaggtttag gcatacctgc tgcattaata
     1261 tctggcggta ttttatgtat tataggagtg actgtatgtt gctatgcctt gcctaaattt
     1321 tggaattatc actcaaagta attaaccaca ttactaatct ccaataatag aggctttttt
     1381 atgttgttgc ttggtagtct catccaaact ggccgcttcc gttaatgcgg ctacattttc
     1441 cttggtaaaa aatccatcaa aattgtgaca tctttcaaca tattcagtaa ttaatatgga
     1501 ataaatggag tgtttggaga aaatttgctt taaatcaggc gaatcctcgc aagccccaaa
     1561 tactttagct aaaaattcaa tatttaattt atctcgaaga gtttcaacga caaaatcaat
     1621 attcttttta ccagaagagt gatccccgtc ttttacttca taagcaatat gatgcattct
     1681 tcttccgtag ttacgcacaa aggtttctgt gggcataggg agattttcaa aggaattcac
     1741 catgaacgga gtgttattag cagtaaacac ttttgccggc gacttgatgt catgtccgtg
     1801 tggatttcta tttacattgg tagaggagtt catatcatag atattataag caccccaaaa
     1861 ataatatttg gataaacata aaaactctaa aatagcatcc tcacgctcac cagccaggat
     1921 tcgagtagcc atatggtcaa tgcctttgat caagtccttg atcccattat cgtggctttc
     1981 ctgatctttc gcatccagca tcgcctcttg ttctttggtt aaataaaatc gttgtcccaa
     2041 ttccagacag tcaaaatcat aaatattatc atcggtatac ccagtgcaat tgtaggtaaa
     2101 atcagataac cgggtaaaat gtatatgttt attacaatag aacttgtttt cagtcatatc
     2161 aggattatga aaattaaaat catgagaaac taatatgtta cgtgtctcat ccttatcctt
     2221 gcatttataa atctcaccta cataacgagc attgaatttg gctcttgagt tgggatacat
     2281 cttgtttaat cttgtgatat cgtctttaaa attaggatcc agtggctcaa gcaccagaaa
     2341 agcaggagaa tttttggtat tgagtaaaca ataaatttta tgcgtgtcac taatataact
     2401 tcgagaatat cgataagggg tcattgagta gagttcttta atatatggaa tagcatctcc
     2461 cgtttgtacc tgaacaacta ccccacacat atcaccaaat agatcctcaa gtccggatga
     2521 taaacgcctt tcgtaaatct tggttttata ttcattaaaa tagtctgaat tgactttatc
     2581 ccctttggga tggtattcaa ttggattaat catcatgttc tccccaattt gattcaccgg
     2641 cacataatat tttaagtata tacctgccaa tgaattttta tttgaattaa taactgtttc
     2701 ctctctttca tggcaaaata gaggtataat aattcatttt gattccttac atactttcca
     2761 atggaagctt taatcgtatc acttggggtg attacccttg cagaaattgg cgataaaaca
     2821 caattactcg ccttccttct ggcagcacaa tttaaaaagc cattgccaat tattttggga
     2881 atattgactg ctactttgat aaatcattct ttggccggac tcatcgggat ctggattacc
     2941 actttattaa aaccgaatgt tttgcgttgg atacttggca tatcgtttat tctgatggca
     3001 atatggacgc tgatacccga tgaaattgaa caggatgaga aaaaaatatc caaatatctt
     3061 ggtgtttttg gagcaacatt tatcactttc tttctgtcag aaacaggaga taaaacacaa
     3121 attgcaacca ttgccctggc agcacactat agctccccga tcctggttat tgccggttca
     3181 acccttggaa tgttgcttgc tgatttacca gctgtttatt ttggaaattt attttcccat
     3241 aaaattccca tgaaaataat tcatgctgtc gctgcatgtg cttttctaat tattggactc
     3301 attaccatct tgttttgacc gactaggcag gattgttatt taacataagt atctagccag
     3361 ttggtatatc tttttaatct gtcgatgaca aagcttggtt tctccaaagt atgatattca
     3421 ttggggtaaa taaccagctg tgtgggcaca tcgagtgatt tcaaagcttg gtataattgt
     3481 tctgacccaa cacaaggaac attaaaatcc aagccactgc acaaaaataa tgtaggtgtt
     3541 tttatattat tcgctttaag aaaagggtag cttaatttta gatacaattc agggttggtc
     3601 cagggcttcc ctaattccag ctcgtattcc ggggtatatt gatcgacacc ataaccagct
     3661 aaaatattcg ctgctccagc acctgatata gccgctttga aacggctatc tgtagctatg
     3721 acataattgg ttagcattcc tccgtaactc catccgccta ttcccaaacg attcggatca
     3781 accattccct tccctatgac ataatcaaca gaagccaata tgtcttttac atccaaatta
     3841 ccccaatctg caaaaattgc ttttgcataa tgaaaccccc ttccggaact ccctcttgga
     3901 ttaggagcaa taacggcata attattggct gctatccact gcatatcaaa gttaaattca
     3961 tggctaaatt gatagactgg tcctccatgc agatagacaa aaccaggaaa taaaataccc
     4021 ggctggtatt ccgctggctt cattaataat ccatcaactc ttgttccatc aaagctattg
     4081 cactgtatgt cttctgcggg ttgaaattta acttcatcca atagcttttg attatgttgt
     4141 gttaaaggaa ttaatccgga attcaaaata aataattcag agggatgtgt atcatctgtt
     4201 gttaacaaaa taattttccc tttggcaagt gaataatcca tgtcatagcg caagccgcct
     4261 gtcaattttt ttatctggcc ggaatgaatg tctatgcgat tcaaataggt gtttctgctt
     4321 tcttctatta atgcataaat aaatttgcca tcttgagacc attttggctt gtaataccaa
     4381 ttatcccgag gtgcaatgat tttctcatct ttagtattga tatcaataat tgccaattga
     4441 ttgggcgaat aataggtcca ttgatgttca ttactgcgaa gataagctat ttttgaacta
     4501 tccgggctcc aggaaagatt tgaatcgtaa tcaggattca tatcactacc ggggaaatga
     4561 gtgagttggt gctcttttgc cccagacttg ggttcgatca aataaatatc ataattaaaa
     4621 tgcctatccg gatcttttcc acgctttgtg acaaacgcga tgtattttcc atcaggtgac
     4681 caggccggta aatattcatc atgatttcca tcagtcagtt gagtaatagc cttactctta
     4741 agttcaacca aatataaatg atctcttccc ttaccaagat aaccgacacc atcaacctta
     4801 aattgatatc tatctacaac tatgggttgt tcttcctcat tagatggatc actcttctga
     4861 gctataaatg caatcctgtt agaatctggg gaccatgtaa aatcgctcac ttcgccatca
     4921 atatgagtta attgatgttc tttattatcc tggacagatg ctatccaaat ttgtgtcgta
     4981 ccctcttttc cgccatcgga taaataagct aaccatttac cattaggact ccatttgggt
     5041 agcgagcttg atgaatcttc tgcaaaggta attcgttgac tggatttact atccactgat
     5101 actttccata cctcagtcgt ggaaatgtta tctttggcat ttaattggga tacggtatag
     5161 gcaacccatt ttccatctgg tgacagatct ggctcgctga tatcttgtat acgataaaga
     5221 tcattaagtt ctggcaaacg cttcccagcc catccatcat gaaatacaac aagacaaata
     5281 attagaccca ggcatacccc cttcatgaca atcatcctta ttatgctgaa aaatcagaga
     5341 tttatagtgc cttaaaaagt atagaataaa tttgttatat atgatttttc ctgcaatcag
     5401 caaacaaatt ttgagtggaa taagtacagt ctggtgtcat tgattaagaa tcgtagctaa
     5461 aattcttcag aaataaagaa atccccgtct gttcaacgag gctcataaaa aggttctaat
     5521 ttatttctgg tatacttttt acggcatgat ccgcttctgt tctttgaaca aactctctaa
     5581 agaaagtctg cgcgctctta cctgatcgat tatgcaatgc attccagtct gaattatgac
     5641 catttgcata ttgtacatca gtctccattt ttctgtcact tttgattttt tccctagctt
     5701 gaactaaagc gtctcttgca gtagccaacc ctggtttcgc aagaggatca gcttttcgaa
     5761 tgatatcatc cagatttgca atttcttcat caatctccgc ctttttcttt tgccgctcag
     5821 aagtcaattc tttatggata gatgcataat gtggaatcac agcatcatcg atacgctcac
     5881 cccttatctc ttttcttgac ctaaccacta tgtaatcttt atatgcttca ggaacactct
     5941 tgaatgcact gcctgcatta atttcccatt tagctaaagc aacaccgaat ttaatgaagg
     6001 gcttagccaa ccatccaagt attgtcccta ctgtgctgtc tttatgacct attgctcgac
     6061 caatttcatc acgctctaaa cgcatatgcc ctactaagaa attagtaata gtagagaaag
     6121 aacggctatt aaatagatta attggctgtc caagttgatg aaaatgttgc gctaccaagg
     6181 aagccacgcc tgcaccaaga gaatgacctt tcagggtaat attttgtggc gaaacacctt
     6241 gatctaatag tctttgcact tgtgcaatac catctgcaac caaatcctca cttgatttgg
     6301 ctttgcctgt actttgaccg acaccacgaa gattaaaacc aataacattg gccttaagag
     6361 ccttcgaatc ttcttttata tcatcaatga tatgttcata acacatacca ttccctacca
     6421 aattgataat atatttttgg tatttgggat cgatactttc ttgtgaccta tgccttactt
     6481 caaacgtatc taaatgcgct ccatcatgag taattacatc atgtttctca caaatcagac
     6541 cgtcttcatt ataattactt acagtatcat cactaaccgc taaatcatca aaattttcat
     6601 tttgtgctgg cagcactaaa ccactaaccc attcaccaag cagtttattt gccccatttt
     6661 ttattaaatc ccataataaa acagggggaa aaaccgttcg aattgcaata aatttaaacc
     6721 attcccaacc actatattta ggttttggtg cattataaat tttagccatg agttcaattc
     6781 tgtttacata aattgttcat attatataca gttaatatta atttaacctt aagctgaata
     6841 ttataaactt aaaaaaatgt cctaatctgg gaagtaaaaa taagtaaagg atgcgtgatc
     6901 gctcaatatt ctcaggactt acgaaaaact ccaaggtcaa ggcaaaaaat gtttttaatg
     6961 agggagttta gataaactaa atgaccaaat taaaaacatt ttttaacata gagattgggg
     7021 tttttcgtaa gtcctgattc tatcattatg ctgacttagc ttatgagctt gagccaatca
     7081 aagacaatgg tgataattaa ccgatgctat tatatgccat cacaataatt tgcttctgta
     7141 atggccttca ttcaactaag aaatgataca aagaatgata gtttggctct ataattgaat
     7201 aatgttgttt tctgcctaaa aagaccttca gctgttccgg gacaggaatt tctttgttca
     7261 atagtggttc taatacctct tcaaatttgg ccgggtgccc tggtgccaca ataacccaag
     7321 gtttgtcctg attgattgta cttagacgat gataagctgt ggcggcatga gggcaaatta
     7381 tataatgata tttattgtaa caatgtagga tagcttgctt aatttgctca tcactaacag
     7441 attctgcatt catctgtttt ctacattcag aataattatt aaataaaatg aataaacgct
     7501 ccaaattgct tggctcacct acatccatcg ctgttgccaa tgttttagta accgatttgg
     7561 cttgataagc tcccgttaac aaaaattcgc ccagtgcact attagtatta ttagctatta
     7621 aaatttgctc aatcggaaaa cccagcattt ttgcccaata acaagccgta acattaccca
     7681 ggtttccgcc tggaactata aaattaacag aatgattatg tcttaatgcc agttgaatgc
     7741 tggtaaaagc atagaaaata atctgtggta ataatctggc tatattcatg ctattggctg
     7801 tagtcaatac tcctttatgt tctcttttgg caaataccag cttcaccagc tgttgacatt
     7861 gatcaaagct gccattaaca gccaatgcac gaatattctc tccccaacaa gtaatttgag
     7921 attgttgtct gaaagtaagt ttatctttgg gaaataaaat gattccttcc aggtctcttt
     7981 tcccatgcaa agcatgagca atcgcagcgc cagtatcacc agatgttgcg accatgactt
     8041 tggcgctctt gttttctatc agatgcaata aacattcact gaaaaattga gttcctaaat
     8101 ctttgaaaga cagggttggg ccatgaaaca actccagtac ataacttttc ttaaaaagat
     8161 gatgtaacgg aaaggagaag gtgaggaatt tttgaaaaaa agcattattt acatcaattt
     8221 tactatctga aaaaaatggc gagagtaaat taacagaaaa gtcgattaaa ttaccgtatt
     8281 cataaaattt ttttattgtt aaattgggaa acctgtcagg tacaaatacc ccaccatccc
     8341 ctgccaggcc tgacagtatc gcttcgtcta ttgtggagac atattgtcct ctcgtactct
     8401 ttattctaat tttcatctgc agcctttgca ccttctgatg aaatattggc aaccaataaa
     8461 tcactttcaa agacaactat tgcttcacgg tatattgctc acatcaatat ttaattcaat
     8521 ctaatttaga agataatcga agtttagttt attttttaga aatttattaa gattgacgtg
     8581 agtggcatca ggaaaaacgc caattcaaac ccaatttgaa ttgttctaaa attggatgct
     8641 tggcgattct ggtcgtttga tacctatgat tattagagtc cttaccctgt gtatcaaagg
     8701 taaaatcttg caaccgattc caatcccact ctacacggca acgccaattt tgagttaact
     8761 ccaactcgag gcctgtacct aattgaactc cattcttttt tttggtattt gaaaatcttc
     8821 cactgaatga gcctaaaggg ccactctgag taatgcttcc gcttaggttg acatcactga
     8881 taatccagcc tacccgtcca tagataattc ccacagaagg caaataaata ccagggtgta
     8941 tagctaaacc agactgccat ttttgtttaa atgaaccctt taatattctt gagtaaagtt
     9001 cgttagaatc aaaaaaatag atttttccct cattggaact gcggttaaaa aaaccttcta
     9061 aaccaaaata ataatgtgtc cggaaaaatt tttcatggcc taaataaatt tctcctccaa
     9121 gtcccgatcc atgccagtca aaatgatcaa agtcagtaaa attgagctct tggctggttt
     9181 cattcctttt aaaattgtaa tgagctgtat ctctaataac tgaaaatcca aaatacgtat
     9241 cgggtcttag aaaaaaattg agctgtgttt tctcagccgc taaaacaaat aaaggtgtaa
     9301 aactgaaaag taaaaacaac aagattgatt gaacaggaaa acctaaatac acgaataatt
     9361 ccttttacta agtcaaaaaa tatccattat cttctattgc acgtcactat tttgcatcaa
     9421 tgacaaaaaa attaattgta cttatctcat gaccaaaact ctatattttt aagaggaaaa
     9481 tctgcataca actatttaat agtttgttac tgtctgttta acagctcata tcaccaaaat
     9541 gaaagacttc ataataatca ataaaatcgc tgtcttatat gcgttctaac cctgattaaa
     9601 tggcatggaa ggatttttga aagctatatt gatatgaaac tatactgttt gattattctg
     9661 gtttaaaacc tggtctgtcc tctccaattt ttgtcacttt attctcgcta tctacatcgt
     9721 agtcagactt aatggttctt tttgctggat ttgtattatt acttgtttga ggtaatactt
     9781 gactgggctg ggtttctgtt tgtgttattt ctgcgttatc ttttggataa gcatttaaag
     9841 cgttttcatc tgttttttta attttctgta atcggctcct caattcttcc aaactagaca
     9901 actcaagagg atcttctgat gcaccatgtt gtttaatacg ttcgcttatt agaaattcca
     9961 ctgtgtccaa acaatcattc acagtagtga gatgcatttc taatttgggc aaatcttcgc
    10021 aatgctcact ttcaagtgcc tcgatttgtt tggttatttc aactaaaagt ggactataac
    10081 ccgactctac tttttcagca tcatcgatta caggtgttga aatagcgctt tgttgtgcta
    10141 ttgcattgag attctcaatc gcatcttgct tcatctctat aatttgtcct tttaaaccag
    10201 aactgattga ggttgttttg ctcatatctt tgtctggtgg gaatgcacta agtgcttgca
    10261 aagcagcggg tatgtctttt ttatcaatag catctttaca ttgctccaaa actggcaata
    10321 ttttacctcg gataaatact ctctcttcaa taagtcgctt tatcgcatcc atctgcgctt
    10381 tagctacttc agactcttgt aagttaaatg ctgaccattt ttcgttatta ggaaactctg
    10441 ctaatacctg ttccaattgc ttaagatcat tcctcaccag ggcagcaaag catttttcca
    10501 taactggatt cacactttta ttgagaaaat tttggtatgc cgttttttca gcactagcct
    10561 gagatgagcg ggaaattata ctgataaaac tactatcaga taattgcgct tgctttttca
    10621 tttccatttg aaaacggaat accattgcca attcattttc ctgatcagca aatgcctctt
    10681 tctcaacagc attttttact gttgctactt tctcactctg ttgaataaaa gctgtttcta
    10741 acttcaaatt ttgtaacgcc aaatcaattg ttaattgaga acgttcttgc tggctagcca
    10801 aaacaggttc tccacctgat ttaaaactcc tcatatgctc catgaaatgt ttggcatctt
    10861 cctgagctgc tggctcagat aggtaatttc taaaggattc attttttaca gcgcttaatc
    10921 ttaagacatc acgacgactt ttaatcaaat caatatagcc ttgtttatcg ggcttatgcc
    10981 catccggtat gtatgcggag acaaagcgtt caatatactc atctggtata aggcaaattt
    11041 ttaacatcgc ctgattgact tcagatctga acacttcatt atttgtaata gttggttgaa
    11101 gtattctttg taaagaaaaa aagctacttt tataatccaa ccaattattg gttgcaaaat
    11161 caaccggccg aggtaatgaa tcaatcactt taggagtaag atcaaatctc tgatctacgc
    11221 ctaacatgga tacgatgcac tgatcaccat ctatcttaat tactctaccg tctttatcta
    11281 atcctatatt cccatttttt aagtcaactt cttgcataaa tactgacaca aaaaccgctt
    11341 gccctaaacc agtaaatgtt ccgtcctcaa agctcttacc ctgaccatag ggtaggtttt
    11401 tgaaaccttc tacttcttca gaataaacat agtaattatt ttgtgcctca tcttttgcaa
    11461 taagcgtttc tggctggtga gggatgatta atctaaaaaa ctcttgtgcg agtacctcgt
    11521 tccttgctga tgcaggatcg ctacacactt tggcaaacca ggtcttggga tttccaggaa
    11581 atgttcctcg tgataccata tggttatggt gtgtagttga cctaaaaata gaattatcat
    11641 tctgaaaatg tttaaaattc agtaaaggta taggcattct tagctccaaa cacatttttg
    11701 atttaactat taaccattag tatagtaatt aatcaaaaat atgtcggaaa atgcctgcct
    11761 tgtgaaataa ttaaacttat ttcataggtc tactgattta tggattgcat gcaagtctgg
    11821 caattatttt ttccgtgtcg cagttatttt aaagcgtacg atcacttcat cttgtatttc
    11881 atcagtactc tcccattcac cttgacctac cccgaatgta ctgcgtttga ttgttgagct
    11941 accttcaact aaagcctgat cgttagaaat ttgatccaca acaaacttca gagtaactgg
    12001 agctgattta tctcttatag ttagaatccc ttctgcttca taggttttat catccaattt
    12061 attaaatttc gaagctttaa attctgcatt aggaaatgtc ttgacgttga accagtcagg
    12121 ggaagccaat gtcgttacga tgtcctcaaa aggtgctgca atcgaattca tatcaatagt
    12181 gatatcaatt gaccctgtct tgtagtttga agggtcagca aaaatttgac cagtaaattt
    12241 cttaaatcgc cctgtaaccg gggcaccatt ttgagtacca gtaaaagtaa tctcactttg
    12301 tgctggcacg atttcccatt cagggagttt actggcataa gtcaatgtat ttacaaaaaa
    12361 tatcaatacc caaagggaag atttaatata gacattcact ataacattct ccgtaatata
    12421 tcatccttat taataaaatg atgttttaat gctgccgctg catgcaggat tatggccata
    12481 attaatgcat accccaacca ttcatgaatc aattggaaca aaattcttct attctcatcg
    12541 ggtgcaacaa ggtcaggtaa aacaaataaa ccaaaaaaag aagcaggcaa tcctgcagcc
    12601 gaggtaatta gccatccact gaagggcata gcaaacatga aaaaataaaa agcccagtgc
    12661 atgctgcgag ctgcaatttt ctctaacagg ggaatagcca attccggtaa ttgattagtc
    12721 aaacgccaaa tcaatctgaa aaaagccaaa aacaatacca gaaatccgta ctccttatgc
    12781 caaccatata attttaattt ctcaagacta atagggatcc tgaccatgta taaacccact
    12841 cccaatagcc caataattaa agcggccata atccaatgga aaaaaatcgc aaccagacca
    12901 aagctggttg aagtattttt taattgcata gattatccat atttcttatc aagataggct
    12961 tctgcttcta tttctatttc aacatcatct cctacttctg gtattaaagc ttttattcca
    13021 taatcagaac gctttaatga agtggtggca ctaaacccag ctgtttctct gtcatttaat
    13081 aaattaattc ctgctttatt taatatcacg ttcaatatga tgggttttgt gacgccatgc
    13141 aaagtaagca ttccatatac ttttgcttta ttctctccca ttacttccac tctgttacta
    13201 acaaaagttg ctgttggaaa ctgcttggta tcaaaaaata atttaccttt caaatgctta
    13261 tccaactcgg gtatacctgt gacgatgtca tcgaccttga tagtaacatt cactttactc
    13321 tgctgcgggt tatccttatc aagaacaagt tgaccacttg cataccattt tccagcttga
    13381 gttgagaagc ccaaatgctt gaccttccat aacacataag tatgctgatt atctaaggtc
    13441 aaggtttgtg gttctgcgta gacataagac gtcaatgcaa agactgccaa tatcgaagca
    13501 ataaaatgcc tgtaaatccg tttcatattc aactcccaaa ttaaattaat gttttattta
    13561 cccatatcca atgatccacc aacaagtttt tcgcaaatcc ctttgggtaa taaaataaaa
    13621 gcatcctttt ggctatccct ggttgaagac ccagcacaat gtgaagttgc tgttgaacaa
    13681 tcattcatcc cttttttaac cacaccataa catttctcct tactcaaatc ctctgctatt
    13741 gctgtgtttg atgcaatgag aactaaaaaa gcacttatgg cagattgtat caatttatcc
    13801 ttcattgaca tggataatct ccttattata gtgaacttaa ccagatttca gcctataaac
    13861 agataactct taagcttccc tggtattttt aataaagtaa actttattaa atcttcaatc
    13921 atcagccttt ctttaatgaa taaatgaatg caaataatca tatgcttttt tggcgtattc
    13981 caaaggatgt gctttttccg gatcctgctc cgcctccact atcatccatc cattatagcg
    14041 atgtttttgc agccctgccc atataggtat gaaatcaatt gctccatcac caggcacagt
    14101 aaatacccct cttcttactg catccataaa gctgagtctc tccatttcaa ctcgtctcaa
    14161 gacctcaggg cgaatgtctt tcagatgaac atacaaaata cgatcaccgt acttatttat
    14221 taaattaacc ggatctattc ctgaaaagac agcatgtcct gtgtcaagta atagagaaag
    14281 caactttgga ctggtattgg tcattaaaaa atcaatgtct ttctcccaaa acaccccagt
    14341 ccctgcatgg taatgataaa cgatcttcat attgaagtcg taagcaattc ggcctaaggt
    14401 atgcagccct tgaatcaata aactccattg ctcttgagta aattgaggga tatgagcact
    14461 taatatcggt aaatcagtgc cttgaatagc atgaccacat tcacaaacat taatatacga
    14521 cgcacctaat gcacgcataa aacttaagtg ctcaagaaat cttgatacgg ttttttcaaa
    14581 agcattggcc tcagtaaaat aagtgctaaa ccatgaactg gataattgta aacctcgttg
    14641 ttccaaagca gcttttaatg ttgttgcatc tcgtgggtat ttattcccca gctcagtgcc
    14701 agaataacct gccaaagcca tttcgttaat acattgctca aaactaatgt ctttacctaa
    14761 attcggatca tcatcattgc accaattaat tggcgcgata ccaagttgaa tactcatctt
    14821 cacttccatc ttcaatattg tctaactgtt tcaatttgct cttgcatcat ttggtaagct
    14881 ttactcactg attctgattg agatacctca gcaactccaa cccgccacca ggtcttatat
    14941 ccttgactca tggtctttgg taacacagga agaacaataa ccgtactatt ttcctgtttt
    15001 tttgctgagt ctaaagcagt ccggaattgc tcataggatt taacaaaata agtggctgcc
    15061 cccaatccct ctgcgtattt acaaaaatct atgggcaaat aatcgccact taaagcatta
    15121 gtagttggct ctctataacg aaattcatta ccaaaaccca gactaccatt cccttcctgt
    15181 aaatttctaa tgcactgaaa tccatgatta tcaaaaatga gaattgtaat tttcttgcgt
    15241 tcctgaatgg cggttaaaag ctctgaatgc atcatgataa aactgccatc cccaactaaa
    15301 acgtagactt ctccttcctg attttctttt gcaatacgta ctcctagacc cgctgcaacc
    15361 tcatatccca tacaggaata agcgtactct aaatgataat cctttgccct tttggattgc
    15421 caaattcgat gcaaatcacc tggcaaactc cccgctgcac taattatcac atcctcttca
    15481 gtaacatact gattgagtag tcccagaaca gcagtttggt gtaacccagc ctgctctgta
    15541 gaataagatt gagtcaattt atctaactct tgggcccatt cattttgata atgacgtatt
    15601 aactgctggt aagagaggga ggtttgataa tctgccagct ctttccccaa ttgctctaaa
    15661 ccagttttag catctccttt taacataact ccattcattt tcatggcatc aagacgtgat
    15721 acattaagat gaatgatttg gcagtcctgg tttttaaaag cccatttgga agctgtcgta
    15781 aaatcctgta atcgagaccc gataaccaaa ataacgtcag cctgagctgc cagagaattc
    15841 gctatttcag atccagtaac acctattcca ccaacgttca tggggtgttt cgctgccagg
    15901 ctactttttc ctgcttgggt ttcagcaaca ggaatgcgat gttttaatgc aaactctgat
    15961 aaagtctccg aagccagtga ataatgcaca ccaccacctg caatgattaa tggttgctta
    16021 gcctttctga tatgccgtat ggcctctgcc aacattgcat cacttatcct ctctctctca
    16081 atacgccata agcgcttttt aaaaaaagat tcctcatatt cataagcttc agactgaacg
    16141 tcttgaggca aacacaaagt aacagtacct gtttcaactg gatcggttaa taccctcata
    16201 gcatgcatac acgctgtcat taactgttca ggtctggtta tcctgtccca aaacttactt
    16261 actggtttaa agcaatcatt aacagatatg gtgtagtcat atggcacctc caattgttgg
    16321 agaaccggat ctggttgcct gcaactgaat acatcacctg gcaataatag taatggaatt
    16381 ctattggttg tcgccgtcgc cgctgcagta atcatattag tagcgccagg accaattgat
    16441 gaagtacaag ccattatccc tagccgattt ttttgtttgg cataggcggt tgctgcgtga
    16501 gccattcctt gttcattatg tccctgataa taaattaaac cctgagcatc gtattccaat
    16561 gcctcaccta atccggttac attaccatgg ccaaatattc caaatacacc attgatgaaa
    16621 cggtattcat tattatccag aacgacatac tgattagcta aaaatcttaa tagggcctga
    16681 gccatagtta atttaatctt catttattta actctcaaaa tcatactgca gataaggatt
    16741 actactgttt ctgttgccat gctcttaaat ttgccctgct tgtccttgtc cattcatggt
    16801 cttttatgaa ggtaggcaag gtatatgcgt tattatttaa atgccggata aaccacagtg
    16861 tataaagtgc ataaccgggc gctgcacaat gagcatgttc ctgattattg gcaatctggt
    16921 aggtatcata gtgttcaatt cgcatcacct ctttaccatt ttcgccaaaa gcatagcctt
    16981 gaggctctga aaacctgtaa tggtaaattt caggttgttg atgataatgt gaaggataac
    17041 ttgaccaacg cccttgaaac gtaataattt ccccaactac caaattagat tcgggtctgt
    17101 ttcgtttatc aaataccgtt ctgacaagtc gatatgaagt attttcaaga actccttctc
    17161 ccctattgtc tagctcaagc atattggatg catcaaataa aatcggagaa aataaatttt
    17221 cgttttccgt ttccattaat aaaatttcac aatcagtcag agcgattact gaacttactt
    17281 cattctttga gcagtgcaag acatacggtg attgagaaaa ataatctgta cgttcaacct
    17341 cctggctata agagccatac tggaaaacaa cttttcctgt cattaagagg gctgcaaatt
    17401 cataacttga gttaaattga tggctttgcc ccttatttaa tttaagagaa ctaaaactca
    17461 ttgctgtagg tgaatcttgc atcgttacaa tcgaatttaa accatcggca aatccacatg
    17521 gatttttaat aagcaactgt gtcttttgat ttttacttaa tttctcatgc agttgtgtta
    17581 actgagagct gctccatatt tcaggatcat gctcatattc ttgaataaaa tagtccaatt
    17641 cttccttgta tggaattgca ggagcgcaac catgacgagt cacgaccaag gcaccacaag
    17701 cattagcata ggttactgct tgctcccatg atttcccttg cagcaaacca gataaaaggc
    17761 cggacataaa accatcacct gctcccaaaa cattcaatac ttcaacagga aaagattttg
    17821 cctgtaatag acctttgtct gttccaaaat gaacacgagc acccttctct cctaatttaa
    17881 caactattgg cgcatcggtt cgctgcctga tattgattaa tgaaacttca atatcattcg
    17941 aaccgcctgc aatacatatt tcctcttcag tcccaacaat caaatcgcat aaaggcaaaa
    18001 cttgttgata cacttgagtt acatattcat tctggcaata acgactttcc ccattaccaa
    18061 tcacagttaa ccgccaaagg acaggtctat aatcgagatc aaaaactatt gtcgtttctg
    18121 cttttcgggc taaatgaact acttccaggg tagtattttt cattgattct gtggataaac
    18181 cagtgcccgt tatcaataat gcctttgcat tttttaaatc agtaaaacta atttgatctg
    18241 atttaatctg catatctgcg caatcattac gataaaaaag cagagggaaa ttggagggag
    18301 gcttaatgcc caaaagtaca aggccagtta aatgattatc tgtttccgat aataactcaa
    18361 tattaactcc ctcacgttcc aattcttctt tcagaaactt acctaactca tctgagccaa
    18421 cacaggaaaa catctggctt tttaatccta atcgggcagc tccaacggca atatttcctg
    18481 cacaaccacc caaatatttt ttaaagcttg caacatcctt caaatccaat cctatttgct
    18541 ccgcgtagag atcaactgct acgcgtccca tacaaatcaa atcgattgct ctattcttat
    18601 caaaataaaa tccgggaaac tgtttcatag cggcctcaac tcaatactac cgggagatgc
    18661 gtttctaaag attgtttcgc tgcttttgca atgcgcaagg cttgtagtcc atccaatcct
    18721 gaaacgggac ttgtctgatt atcattccag gcagtataaa acgcattgag ttctgccaga
    18781 taagcttgat gataacgctc aaggaaaaaa tattgaggtt ttgcgccatc cgttccatta
    18841 gccgagtatc gagataccgt atgctccaat tgattttcag ccaataacat accagctgaa
    18901 ccaaatactt ctatcctttg atcataccca tagattgctt gacggctgtt atctatgaca
    18961 ccaaatgcgc cattagcgaa ccgtagttga atgattgctg tatctacatc atcaaactgc
    19021 tcaaaatcag gattaattag aacagcccct gttgcataaa cctccacaac ctcggattgc
    19081 attaaaaaac gtgccatatc aaaatcatga atagacatgt ccataaatat tccaccagag
    19141 gtcgcgcaat actctttgga tggacaagct ggatcacgag aagtaattcg aagaatatgg
    19201 ggagtgccaa tttcaccaaa ctgaactctt ttctgaatgc tggcaaaact tgggtcaaat
    19261 cgacgattga atccaagttg caataaagta ttattgtttt ttattatatt t
//
LOCUS       DATNSN010000048        19207 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.048, whole genome shotgun sequence.
ACCESSION   DATNSN010000048 DATNSN010000000
VERSION     DATNSN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19207
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.048"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            165..563
                     /locus_tag="VAH76_001397"
     CDS             165..563
                     /locus_tag="VAH76_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386032.1"
                     /translation="MFNKFLVTALSVVTLTLSPYGFAKDDSCEVQVAVSTPPVQFDQS
                     VAFNVTNDDGISKSIILQGGSSPKTIGKLICSPLPYTISATQYFTPTGLTNRHAQPVG
                     QCVLKAGNVILNGSNNSVAVVFPNDFICHH"
     gene            complement(604..1791)
                     /locus_tag="VAH76_001398"
     CDS             complement(604..1791)
                     /locus_tag="VAH76_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEO1386033.1"
                     /translation="MVFGFLNLSFWGYVLATFILTQITIASVTIYLHRNQTHRALTLH
                     PVISHFFRFWLWLTTGMVTADWVAIHRKHHATTDVEGDPHSPVVEGIRKVFWQGAELY
                     REARKDRDMVAKYSHGTPTDWIERNVYSRHSSKGILLMLLLDLFFFGIPGITVWAIQM
                     MWIPLFAAGVINGIGHYWGYRNFECPDAATNIIPWGFWIGGEELHNNHHTFASSAKFS
                     VKWWEFDIGWMYIRILSFFGLAKVKKLPPKLAMDEGKFHVDLDTVRAVISNRFQVMSN
                     YYKNVIRPILKYEKNTSIETKEDKKLFQRAGRLLRREDKLLSTKAKTRLQKLLDAREQ
                     LRVVYSYKQSLQNVWLKTATTQKELVEALQQWCRQAEESGLDVLKQFAQQLKGYVPVY
                     TRE"
     gene            1972..2202
                     /locus_tag="VAH76_001399"
     CDS             1972..2202
                     /locus_tag="VAH76_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386034.1"
                     /translation="MSDKVQKLIENIVPFIVIGVGIAIVVALLFMFFYVAIWGVIIGG
                     ILWLGALAKEYFFPSKSSQKEQGRIIEHDDKK"
     gene            2202..3026
                     /gene="mutM"
                     /locus_tag="VAH76_001400"
     CDS             2202..3026
                     /gene="mutM"
                     /locus_tag="VAH76_001400"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEO1386035.1"
                     /translation="MPELPEVETTKQGIKPHLEGRMITAVQVRNRKLRLPVPLNLNEL
                     CQGKHITAITRRGKYILLHMDKGYLLIHLGMSGHLRIVSQTANPQKHDHIDLHINNGL
                     ALRFCDPRRFGLFIYIDENPYQHPLLAHLGPEPLSDDFNSEYLLRKAANKSQSIKSFI
                     MDSQIVVGIGNIYAAESLFLAKIHPNTSAKKITTEEFNALTGHIKKILESAIEAGGTT
                     LRDFYSSDGKPGYFRFALKVYGRKNLPCLVCENKIETVVIAGRHSAFCPHCQPIIT"
     gene            3101..3721
                     /locus_tag="VAH76_001401"
     CDS             3101..3721
                     /locus_tag="VAH76_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386036.1"
                     /translation="MDIKKLKKAASNFLERYPGGFYHPEMIEMGKKHKMDKMVALAQG
                     SFAKQNFKNPDEITNSMLKIVTQSSLISIFEKPKFRDFIKRTPPNDKVLIAKGLKELL
                     YGNEQEGFEELLFQFQREKLAKWTLMTIFQAYFNPEVDVFIKPTTTKNIIKELNLDIV
                     YNPTPSWEFYESYRNIINQMKTKIEKSLAPSNAAFCGFLMMSLDDR"
     gene            complement(3779..3849)
                     /locus_tag="VAH76_001402"
     tRNA            complement(3779..3849)
                     /locus_tag="VAH76_001402"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3815..3817),aa:Gly,seq:ccc)
     gene            4161..5228
                     /gene="dinB"
                     /locus_tag="VAH76_001403"
     CDS             4161..5228
                     /gene="dinB"
                     /locus_tag="VAH76_001403"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO1386037.1"
                     /translation="MNPIRKIIHIDMDCFYAAIEMRDFPELANKPIAVGGDAKRRGVI
                     ATCNYAARQFGIRSAMPTAHALKLCRELILRPVRMDVYQKESQYIRSLLTEYTDLIEP
                     LSLDEAYLDVTESTQCQGSATWIAEEIRARIYQTRQLTASAGIAPNKSLAKIASDWHK
                     PNGQMVIRPEDVSAFVLDLPVRKLFGVGPKMEEKLGALNIKTCADLQRYSVEYLLQKF
                     GTMGQRLYELARGIDNRPVNPERIRKSISVEETYPKDLPNSEACLAVLPELMARLEAR
                     IQRAGKISGIHNLFVKLKFNDFQQTTIERVMDKLDLIILRQLIQEGFARRGMPVRLLG
                     IGIKLKQENTYQSVQLPLLDL"
     gene            5472..5926
                     /locus_tag="VAH76_001404"
                     /pseudo
     CDS             5472..5926
                     /locus_tag="VAH76_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214785.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            6069..6389
                     /gene="sugE"
                     /locus_tag="VAH76_001405"
     CDS             6069..6389
                     /gene="sugE"
                     /locus_tag="VAH76_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEO1386038.1"
                     /translation="MLLPWLYLIIAGLLEVCWAVGLKYTDGFSKWQPSLFTVTMLIIS
                     MFLLAKAAQTLPIGTAYAVWVGIGSLGTAIMGILLFQEHLSVFRLLYLVLMLISIFGL
                     KYTA"
     gene            6458..7189
                     /locus_tag="VAH76_001406"
     CDS             6458..7189
                     /locus_tag="VAH76_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO1386039.1"
                     /translation="MKISRLKTFWIITRLFYYTGVACTRSIFKYLFSTPTRPWVDKVI
                     HHWVDQLLNAVKVEYKVINPHNVQPQPGKATILMCNHSSAYDIPLGFKAFPNNSIRML
                     AKRELSKIPLLGKGMAAAEFPFIDRKNRFQAIKDLEFAQQLMESGIILWVAPEGTRSK
                     DGKLAPFKKGGFITAIQAKATIIPIGIRGAFDILPARTFNIHLNQKAEIHIGEPIDAA
                     QYTIENKEELIEKTHQSIKKLVGEN"
     gene            7399..9615
                     /locus_tag="VAH76_001407"
     CDS             7399..9615
                     /locus_tag="VAH76_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1386040.1"
                     /translation="MSINKIEEDRDTSSDLEMKAKMYLVHRMDKARPLTVKDVHILIN
                     KLSKDLQKNQLTLIDCQLIINLVESRFTLVKQATRYLQFFLNKLDGKSINPILIPLSN
                     IPFWQNEPHPFDHYRSTDQLPEKADIVIIGAGLTGASTAYHLIDAVKKRNLTVVLLDK
                     GNPATEASGRNGGNFELLPENSVGIYSGLVSERFRFLKRCYPLVHPSILQIESEYHAS
                     LVFQFALKNRNRFKEIVRKERIYCDLAAKGWLYIAHTEEEEQAICDEVTLAAQHGERI
                     YIWSRKKIREQFGINSKFIGRFIPDDGTYNPFKYTCCLIKAAIKGGIKLYTFVKVQQI
                     QSMDTEYHQLKTNMGLLKARQVIVATNAFTSELLPELSAIKPHQSQIAITDSTPDYCK
                     GRLITSEDGPLYLNQPRVRSKNGLVPLLIGAGDDRPMKNPYYRKRSKKVHDLIVQQRD
                     KYFPDLKGKPFSTEWVGALAFTPDQLPAIGYLSPGIIIVMCCNGYGGSYTTAAGFAAA
                     EIALTGQNPPWLPADVFSPKRLLSDKPLFLEDSDSLWRIGKTLCTTLNNLNQLLAESL
                     SYQSMYQPYFTISSLPHGHQEKTCNLDPLRVMHQLAIFRTFAYQELEMLIRYTSPVCL
                     NKGEILFKQGDAGHSCFILIQGKINLYYEHAEGFTSMVAELEAGSVFGQMALFLENKR
                     MATCQATENCSMLEIMVEPCKSLFIQQQALGIKLLRLLNQGVIDALHKLNKRLKYT"
     gene            complement(9713..11437)
                     /gene="ggt"
                     /locus_tag="VAH76_001408"
     CDS             complement(9713..11437)
                     /gene="ggt"
                     /locus_tag="VAH76_001408"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEO1386041.1"
                     /translation="MKYNYKWLSCLFFLICNIYQVNAKTFPQHPPGYAVASAHPLATH
                     AGLETLAAGGNAFDAAVAVAAALAVVEPYHSGLGGGGFWLLHQEKEHLNLFIDGREVA
                     PLAASKDMYLAPDGQVIPGLSLNGGLSAAIPGQPAALVYIAKNYGRLPLSKTLAPAIK
                     LAEEGFPVDPQFRYFSTLDDRLENLKKYPDTAAIFLKNGQPFEIGEKLVQKDLAKTLR
                     LIAKYGNAGFYSGETASLLVQGVNAAGGIWSLDDLAQYKIKVRPPLISAYHNMLIITA
                     PLPSAGGVALVTMLNILSKFPLSSFPKLKWVHYVVESMRLAYWQRDQFLGDPDFIKVP
                     LDKLISSDNAKQLSRLISPNKAIDSKVLQGSVPTGKHSNTSHISIIDTEGNRVAATMT
                     INYIFGSSVVAKGTGVLLNDEMDDFSTKVGEQNVFGLVGSERNAIEPRKRPLSSMAPT
                     FLEMPGRVAILGTPGGSRIPTMVLLASLLFYDGYGAISMVSAMRFHHQYLPDVLQFEP
                     DAFPSSIQQGLQAMGYHLMPLKQYYGDMQAITWDKESNVLTAASDPRHIGLAAAISNM
                     AYGYGVNY"
     gene            complement(11427..11933)
                     /gene="coaD"
                     /locus_tag="VAH76_001409"
     CDS             complement(11427..11933)
                     /gene="coaD"
                     /locus_tag="VAH76_001409"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO1386042.1"
                     /translation="MQMVINEMKQKAIYPGTFDPVTNGHIDIITRASTIFPELIVAVA
                     SNKNKRPYLSWETRISLLEESVGHLTGVRVVGFDNLLIDFVLEQSAGIILRGLRAVSD
                     FEYEFQLAGMNRKLSKKVETLFLTPAEHLMYISSTLVREIAALNGDISQFVPPNVVRE
                     LKKRQNEI"
     gene            11996..12595
                     /gene="lolB"
                     /locus_tag="VAH76_001410"
     CDS             11996..12595
                     /gene="lolB"
                     /locus_tag="VAH76_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEO1386043.1"
                     /translation="MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETAT
                     VSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIRLMGPLGGGAVLISKKGNTITFQ
                     DGSKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGVQSEKHDQFNHLIQLKQN
                     GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV"
     gene            12723..12797
                     /locus_tag="VAH76_001411"
     tRNA            12723..12797
                     /locus_tag="VAH76_001411"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12755..12757,aa:Gln,seq:ttg)
     gene            12977..13924
                     /locus_tag="VAH76_001412"
     CDS             12977..13924
                     /locus_tag="VAH76_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEO1386044.1"
                     /translation="MSTMMIFTGNANPELALKISSHLQIPIGKATVGTFSDGETMVEI
                     LENVRGKDVFVLQSTCAPANNNLMELLIMADALRRSSAGRITAVVPYFGYARQDRRVR
                     SARVPITAKVVADMMASVGICRVLTVDLHADQIQGFFYMPVDNVYSTPVLLEDITKQK
                     LNNIMIVSPDVGGVVRARAVAKRLNDAELSIIDKRRSGPNKSEVMHIIGEPANKNCII
                     VDDIVDTAGTLCTAAQELKKNGAKSVRAYITHPVLSGPAVNNIKHSGLDEVVVTDTIP
                     LSAEAQNCEKIRVVSLADMLAQAIKRVNVEESVSSMFAE"
     gene            complement(13986..14267)
                     /locus_tag="VAH76_001413"
     CDS             complement(13986..14267)
                     /locus_tag="VAH76_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEO1386045.1"
                     /translation="MTTISNDAGDTTASLAESVTQSVQKYFSELKGTDPVDLYQFVLE
                     EIETPLFRAVMEHCKYNQSRAAIMLGISRGTLRTKLRRYFDDKYVGTRD"
     gene            complement(14457..15914)
                     /locus_tag="VAH76_001414"
     CDS             complement(14457..15914)
                     /locus_tag="VAH76_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEO1386046.1"
                     /translation="MKKLTGLVIILAVLILGGYYGMGILTERTVKKTVEVINQSNGLF
                     ADIQQYKRGWFCSDALVKWRLHVPERVVKDDDGKLQTTPAQDYQMEMPIKIYHGPIIY
                     ANKQLRFGMGFAQTVFPFPEKYNQQFNELFSQDSEKPQLDLSIFVNYLNKSTVELSIP
                     SFKLTAKDGNGKFNWMGMDSTTSMSSNLDKVEGEIEFDGAEFSKDDTKLSLGKVISEY
                     DLHKTTTGLYLGQASFSLPSLAVVVKDQKMFEISELTLSSSSDIDDNLFSTHFSAALK
                     SLFANGQNYGPGEVEMSLRNLDAEVLAKINEQANAMQRGTDQERQQALMAMLPELPKL
                     FSKGAEFEISKLSLKLPQGMIDGNLLITLPKGDNSNPFELIQKTQGNAKLKMPIEVVK
                     QLMQQSIMQQMAKQPEMQQALIQQLQSSQGQSNQAEPTPEQIAAMQVDKQINELEQSG
                     LITVDGKDYVIEMSLSEGKFTVNGKPFDPSMMKFQ"
     gene            complement(16142..17425)
                     /locus_tag="VAH76_001415"
     CDS             complement(16142..17425)
                     /locus_tag="VAH76_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386047.1"
                     /translation="MGISKVEIYKRKNYLGFAWLVWGLAAAFYFSDYLARVAPGVMHR
                     YLQMDFGINEAGFGILTASFYVPYILMQIPVGLTVDRLSIRWLLTIMSLVTAFGCCVF
                     GLADGLLTASIGRMLIGFSAAFAFICSLRLATSWFPPTMLGLLSGLTQALGMLGAAAG
                     EAPVSFLVSNVGWRHSMLIIAFLFIALSGLLYQFVQDKPGEHRNEIRSVNRISILDSL
                     KIILSNKQTWLNAMYAGFLFGPTAVIGEAIGPAYLQFGRGLGAHAAAFATGLIFIGWG
                     ISGPLSGWISDKMGRRKPLMIISAVCGVILSSLFVFIPEMSQTTAYILFFVFGLTNTG
                     VAISYAVSTEIHDRSVVGTSIAFTNMTSIFVGALFQPLVGRIIDMVSGPRAYNVETLL
                     LSDFQAGLKLLPLCSLVALILAFTVKETYCKPIRH"
     gene            18074..18463
                     /locus_tag="VAH76_001416"
     CDS             18074..18463
                     /locus_tag="VAH76_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO1386048.1"
                     /translation="MNKLNIAIKEAFESSGSSKEANKVYLEFIKANFIIPIESNSDDS
                     EPRVLFLEENNHAFLPVFTDMKYLDAWASEISQHIKLLKLSGVDLLKGLGEKVTVCLD
                     IGSELYKEFNPSEIERMRSMVLKLFKD"
     gene            complement(18534..18905)
                     /locus_tag="VAH76_001417"
     CDS             complement(18534..18905)
                     /locus_tag="VAH76_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmission trait enhancer LetE"
                     /protein_id="HEO1386049.1"
                     /translation="MNDTTSLLSHLRLRFNIEHPSYEECYIFGYECALAEVPEEDNPF
                     REGSQEAEQWLEGWWDGIYGEKPLFDLNNIEEDILLDKTTAANDQQYHHKNSFLSLVF
                     EIGGAIAASAIVGYQLLELVA"
BASE COUNT         6158 a         3796 c         3519 g         5734 t
ORIGIN      
        1 agctacttta tttttgatca tgttacggat ctgatataaa tgtattaatc atgactaatt
       61 tgcattacaa atcgaatgaa gataccctaa atctgctttt aagtaagcat cgtaatataa
      121 gtgtttttta atattagaag tagcattcag agaatgaggg tattatgttt aataagtttt
      181 tagttacagc actatccgta gtcacattga cactttcacc atatggtttt gccaaagatg
      241 attcatgcga agttcaagtg gctgtttcca ctccccctgt tcaatttgat caaagtgtgg
      301 cgtttaacgt aacgaatgat gatgggatta gtaaatccat tattttacaa ggcggaagca
      361 gtccaaaaac tatagggaaa ttaatttgtt ctccactacc ttataccatt agcgccaccc
      421 aatattttac accaacaggc ttaaccaata ggcatgctca acctgttggc caatgcgtat
      481 taaaagctgg aaatgtcata ttgaatggtt ctaacaatag cgttgctgtt gttttcccca
      541 atgattttat atgccaccac taaaatatta gctgcattgt gcattgatgg ggagtgcaaa
      601 acatcactcc ctagtatata ccggtacgta gcctttcaat tgttgagcaa actgtttcag
      661 cacatcaaga cccgattcct ctgcctggcg gcaccattgc tgcagagctt ctactaattc
      721 tttttgagtc gttgctgttt tcagccaaac attttgtaat gattgcttgt aagagtaaac
      781 cactctcaac tgttcgcggg catcgagcag tttttgcaaa cgagttttag cttttgtcga
      841 taataactta tcctctcgac gcagtaatct acctgcccgt tgaaacaatt tcttgtcttc
      901 ttttgtctca atgctggtat ttttttcata cttcaaaata ggtctgatga cattcttata
      961 gtaattagac atcacttgaa agcgatttga aataactgct ctcacagtat ccagatcaac
     1021 atggaatttt ccttcatcca tagccaattt gggaggtaat ttttttacct tggccaaacc
     1081 gaaaaaggaa agaatgcgaa tatacatcca accaatatca aactcccacc atttcactga
     1141 aaatttggct gaggaagcaa aagtgtgatg attattatgt aactcttctc caccaatcca
     1201 aaatccccag ggaatgatat tagtagcggc atcagggcat tcaaaattcc tgtatcccca
     1261 ataatggccg atgccattga tgactcccgc agcaaacagc ggaatccaca tcatttggat
     1321 tgcccatact gtgattccag gaatcccgaa gaaaaataaa tccagtaata acatcagcaa
     1381 aatacctttg cttgaatggc gcgagtagac attacgctct atccaatcag ttggtgtgcc
     1441 atgagaatat ttggcaacca tatccctgtc cttgcgtgcc tctctgtaaa gttcagcgcc
     1501 ttgccaaaat acctttctga ttccctcaac cactgggcta tgagggtctc cctcaacatc
     1561 agttgttgca tggtgcttgc ggtggatagc aacccaatcc gcagtaacca tacccgtagt
     1621 taaccataac caaaaacgga aaaaatgact gatcacagga tgtaatgtta gcgctctatg
     1681 agtctggttc cgatgaagat agatagtaac agatgcaatt gtgatttgag tcaggataaa
     1741 agttgcaaga acatatcccc agaaagacaa gtttaaaaag ccgaatacca taataacccc
     1801 acattggacg gaattataaa aataactata atttaagagt gaatgtattt tattcctaaa
     1861 ttatagaatc aatatatgtg taatgataac acatttttta tcatttcatt gcatgatttc
     1921 atgatgtagt cgataataaa tttactcatc ttattttatc aggatataga catgagcgat
     1981 aaagttcaga aattgattga aaatattgtg ccttttatag tgattggtgt tgggattgca
     2041 atagttgtag ccttattgtt catgtttttt tatgtggcta tttggggcgt aatcatcggt
     2101 ggaatactct ggcttggggc cttagctaag gaatatttct tccccagtaa atcatcccaa
     2161 aaagaacaag gaagaattat agaacatgat gataaaaaat aatgcctgaa ttacccgaag
     2221 ttgaaacaac aaagcaaggt attaaacccc atcttgaagg ccgtatgatt accgcggtgc
     2281 aagtgcgtaa tcgaaaactt agattaccag tacctctaaa cctgaatgaa ttatgccaag
     2341 gaaagcatat aacagctatt acccgtagag gaaaatacat cttacttcat atggataaag
     2401 gttatcttct catccatctt ggaatgtccg gacatttgcg tatcgtctcc cagacagcaa
     2461 atcctcaaaa acatgatcat attgatctgc acattaataa tgggcttgct ctcagattct
     2521 gcgatccaag aagatttggc ttatttattt acattgatga aaatccttac caacacccac
     2581 tgctggcaca tttaggcccg gaacctctgt ctgatgactt taatagcgag tacttattac
     2641 gtaaagctgc gaataaatcg caatccatca agtcatttat tatggatagc caaattgttg
     2701 tcgggatagg taatatttat gcagcagaga gtctttttct tgccaaaatc catccaaata
     2761 cctctgcaaa aaagataact acagaagaat tcaatgctct gactggtcac atcaaaaaga
     2821 tacttgaatc tgccatagag gcaggaggga cgacacttcg tgatttctat tcttctgatg
     2881 gtaaaccagg gtactttcgt tttgctttaa aggtttatgg caggaaaaat ctcccctgtt
     2941 tggtatgtga aaataaaata gaaactgtcg tgattgccgg gcgccactcc gcattttgcc
     3001 cgcattgcca gccaatcatt acataataag tttaataatg gccacctgga accatttttg
     3061 tttcttaatt gattctgttg ttgtgacaaa aggttaattt atggatatta aaaaacttaa
     3121 aaaggcagcc tctaattttc tggaaagata tccaggaggt ttctaccacc ctgaaatgat
     3181 tgaaatgggt aaaaaacata aaatggataa gatggttgct ttggctcaag ggagttttgc
     3241 caagcaaaat tttaaaaacc ctgatgaaat aacaaattcc atgttgaaaa ttgttactca
     3301 gtcatctctg atttcaatat ttgaaaaacc taaatttaga gattttatta aaagaactcc
     3361 tccaaacgat aaagtactta ttgcaaaagg attaaaagaa cttttgtatg gaaacgaaca
     3421 agaaggcttt gaagagcttc tttttcaatt tcaaagagag aaattggcga agtggacttt
     3481 aatgactatt tttcaggctt acttcaatcc cgaagtggat gtgtttataa aacctacaac
     3541 aaccaaaaat atcattaaag aactaaacct tgatattgta tataacccaa ccccttcttg
     3601 ggagttctat gaatcttata ggaatatcat taaccaaatg aaaacaaaaa ttgaaaaatc
     3661 actagcgccc agcaatgcgg cattctgtgg ttttttaatg atgagcctgg atgatcgtta
     3721 aattttctat gagtatcgct tgatgggctc tatgaagaca tgccacattt ttttattgag
     3781 cgggtgatgg gaatcgaacc cacgccatca gcttgggaag ctgaggttct accattgaac
     3841 tacacccgca aaaactcacg acccctttcg tcaatagaat attaaggtac cattttgtag
     3901 ttattgattc aattaagtta tcacaactgc gatttaaatt taaaaacaga ttttaaaact
     3961 caactattaa cttaaactat acaaatgtat ggcaagcata atttacagaa catagcttgg
     4021 ttgcttcttt tgattgcaat cttatattga tatctaaata aagtaaaaaa tagttattct
     4081 gtagactaag ctttacagct tcaagaaaat cagctaagac ctaatatgaa acctctgtta
     4141 ttgcaatctg tgattgaaaa atgaatccga taagaaaaat tatccatatt gatatggact
     4201 gtttttatgc ggcaattgaa atgcgtgatt tccctgagct ggcaaataaa cctatcgctg
     4261 taggcgggga tgcaaaacgt cgtggagtca tagcgacctg taattatgct gcccgccaat
     4321 ttggcatacg ctctgcaatg ccaacagccc atgcgcttaa actttgccga gaattaatct
     4381 tgcggccagt acgtatggat gtataccaaa aggaaagcca atatattaga tccctattaa
     4441 cagaatatac agacctcatt gaacctttat cattagatga agcctatctg gatgtcactg
     4501 aatccacaca atgccaggga agcgccacct ggattgctga agaaatacgc gcccgaattt
     4561 atcaaacaag acaactcact gcgagtgcgg gcattgcgcc aaataagagt ctcgcaaaaa
     4621 tagccagtga ttggcataaa ccgaatgggc aaatggttat tagacctgag gacgttagcg
     4681 catttgttct tgatttacca gtacgcaagt tatttggtgt agggccgaag atggaggaaa
     4741 aattgggtgc gctcaacatt aaaacctgtg ctgatttgca gcgatattcc gttgaatatt
     4801 tattacaaaa atttggcacc atgggtcaaa gactttatga attagcgcga ggcattgata
     4861 atcgccccgt caatcctgag cgtattcgaa aatccatcag cgttgaagaa acctatccaa
     4921 aagacttgcc aaatagtgaa gcttgcctgg cagtcttgcc agagcttatg gcgcgccttg
     4981 aagcaagaat acaacgtgca ggtaaaattt ctggcattca caatttattt gtaaaattga
     5041 agtttaatga ctttcagcaa acaactattg aacgagttat ggacaaactt gatttaatca
     5101 ttttacgtca attgatacaa gaaggttttg ccagaagagg catgccagta cgattattag
     5161 gtataggaat taaattgaaa caagaaaata cgtatcaaag cgtacaattg ccattacttg
     5221 acttataaaa agtatctaaa cagttgatat cagattcttt cccaaacttg ccataattat
     5281 cagaacttaa tacatcagct ctctttttta tgcaacatcc tgcaatagtt aacaaaatca
     5341 ggacttgtat attgaaaata tcaatcaact gatgtgcgca ctgtagtttt cataacataa
     5401 acaaatagat aggataaatt taaaaatagt cttattattc ttgtataata acacttggta
     5461 atattatttt gatgcagcca ttaattatcg atgaaaaact ggtttgtcgt ttaattgcga
     5521 gccagtttcc ccattattcc aaactccctg ttagcccggt ggctgtcggt ggatgggata
     5581 accggacgtt tcatcttggc aagacacacc tgtgtgggtt catggtgata tcagcgcggg
     5641 taatcttctg gttaaaaatg ggaaattatg cgctgttatc gattttgggc agttgtccgt
     5701 tggcgatccg gcgtgtgatc ttgctattac ctggacgtta tttgcaggag acagcagaaa
     5761 aatcttccgt gaaatgcttg ctttagacaa aggcacttgg ttccgtgggc aagcatgggc
     5821 tttatggaaa gcgttaatcg tagcagcagg cattgtaaac accaatgcaa tggaactgca
     5881 aaaatcctgc cgtattatta atgagctatt cttgactgag ctataatcta taccatatcg
     5941 ctcaatttga ctcagggaat actattttct tgaaggcaat gatatgctat aaaataatct
     6001 ccggacgaga cgcgccgtac ccggttaaga caagacgacg cctttaatca atctaataag
     6061 gagtcgttat gctactaccc tggctttatt taattatcgc gggacttttg gaagtctgtt
     6121 gggctgtcgg tttaaaatat accgatggtt tttcgaaatg gcagccaagc ctgtttacag
     6181 tcaccatgct gattatcagt atgtttttat tagccaaggc cgcccaaaca ttacctatcg
     6241 gcactgccta tgcggtatgg gtagggatag gttcgctggg gactgcaatc atgggaatac
     6301 tgctgtttca ggaacatcta tctgtcttcc gattgctata ccttgtatta atgctgatat
     6361 ctatctttgg tttaaaatac acggcttgat ttgaatcgtt ccagtattcc tcaacttgtt
     6421 gtatgatggc cctctcataa tcccatgcgc tctaacgatg aaaataagtc gattaaaaac
     6481 tttttggata attacccgcc tattttatta cacaggtgtt gcttgcactc gctctatatt
     6541 taagtatctt tttagtactc ctactaggcc atgggttgat aaagtcattc accattgggt
     6601 tgatcaactc ttaaatgctg tgaaagtaga atataaagta attaatcctc ataatgtcca
     6661 gcctcagcct ggcaaggcaa cgattcttat gtgcaatcat tccagtgctt atgatattcc
     6721 tttaggcttc aaagcattcc ctaataactc tattcgtatg cttgccaaac gagaactctc
     6781 caaaattcca cttttaggaa agggaatggc cgcagcggaa ttccctttta ttgacagaaa
     6841 aaacagattt caagccataa aggatttgga atttgcccag caactaatgg aaagtggaat
     6901 tattctatgg gttgcaccag aaggaactcg ctcaaaagat ggcaaactag ccccatttaa
     6961 aaaaggtggc tttatcactg ctattcaagc caaggctact ataattccca taggaattcg
     7021 cggggctttt gatattctcc ctgcccgcac ctttaatata catttgaatc aaaaagcaga
     7081 aattcatatt ggcgagccta ttgatgcagc tcaatataca attgaaaata aagaggaatt
     7141 aatcgaaaaa acccatcaat caataaaaaa gctggttggg gagaattgaa aaaactcaca
     7201 ttccagtttg ctgaaaaaga tgactaaaaa tacctacaat aactagaaaa aaaagaccac
     7261 gttatacaaa aaataaaatt gacttctaca atagaatgaa caatccaggc tacctgtctt
     7321 ttaatatcgg tactaactgt tttgaaattt cctaaaactt cacctagact ttaaaaagaa
     7381 aatcaatatg accatgccat gagcattaat aaaatagaag aagacaggga tacctcttct
     7441 gatctggaaa tgaaagccaa gatgtactta gtacaccgaa tggataaagc caggccatta
     7501 acagttaaag atgttcacat cctgataaac aagttaagta aagatttgca gaaaaatcaa
     7561 ttaacattaa ttgattgtca actaataata aatcttgttg aatctcgctt tactttggtc
     7621 aaacaagcaa caagatatct acagtttttt ctaaataaac ttgatggcaa atcaattaac
     7681 ccaatcttaa tcccactttc aaatataccc ttttggcaaa atgaaccaca tccattcgat
     7741 cattatcgat ctaccgatca actacctgaa aaagcagata tcgtaatcat aggtgcaggt
     7801 ctaactggcg cttctaccgc atatcatctg atcgatgcag ttaagaaaag aaatcttact
     7861 gttgtattgt tagataaagg aaatccagcc actgaagcca gtggtcgcaa tgggggtaat
     7921 tttgaattat tacctgaaaa ctctgtggga atctatagtg gtttagtctc agagcggttt
     7981 cgttttttaa aacgatgcta cccccttgtt catccgtcaa tattacaaat tgaaagcgaa
     8041 taccatgcct cgctggtttt tcaatttgct ttaaaaaatc gtaatcgctt taaggaaatt
     8101 gtcagaaaag aacgtattta ttgtgatctg gcagcaaaag gatggctcta cattgctcat
     8161 actgaagagg aagaacaagc aatttgtgat gaagtcactc tggctgccca acatggtgag
     8221 cgcatctaca tttggtctcg aaaaaaaatt agagaacaat ttggcatcaa ctccaagttc
     8281 ataggtcgat ttattccaga tgacggcacc tacaatccct ttaaatatac gtgctgtctg
     8341 attaaggcgg caatcaaagg tggtatcaag ctctatacgt ttgtgaaagt tcaacaaatt
     8401 cagagtatgg atacagaata ccatcaattg aaaaccaata tgggtttgct taaggctcgg
     8461 caagtgattg ttgcaacaaa cgcatttact agtgagttgc tcccggaatt gagcgcgatt
     8521 aagcctcacc aaagtcaaat tgcaattacc gattcaacac cagattactg taagggtcga
     8581 ttaataacta gcgaagatgg accgctctat ctcaatcaac ccagagtcag atcaaagaat
     8641 gggttggtgc cattgcttat tggtgccggt gacgaccgac caatgaaaaa cccttattat
     8701 cgcaagcgtt caaaaaaagt tcatgatctt attgtgcagc aaagagataa gtatttcccg
     8761 gaccttaaag ggaaaccatt ttcaaccgag tgggttggcg ccttagcttt tacacctgat
     8821 caattacccg ctattggtta cctgtctcca ggaataatta ttgtgatgtg ctgtaatgga
     8881 tatggaggaa gctataccac agcagcagga tttgccgcag ctgaaattgc attaactgga
     8941 caaaatcccc catggctacc cgcagatgta ttttctccta aacgcctcct gtcagacaag
     9001 ccactatttt tggaagatag tgacagttta tggcggattg gaaaaacact ttgtacaaca
     9061 cttaataatc tcaatcaact tctggctgag tccctttcat atcagtccat gtatcaacct
     9121 tattttacaa tttcttcatt accgcatggg caccaagaga agacttgcaa tcttgaccca
     9181 ctaagagtaa tgcatcaatt agcaatcttt agaacttttg cctatcagga attagaaatg
     9241 ttaattcgct atacctcgcc tgtttgctta aataagggag aaattctttt taaacaaggt
     9301 gatgcgggac attcttgttt tattctgatt caaggaaaaa tcaatcttta ttatgagcat
     9361 gctgaaggct ttacttcaat ggttgcagaa ctggaggccg gatcagtatt tggtcaaatg
     9421 gcattatttc ttgagaataa acgtatggcc acttgccaag ctacagagaa ctgttccatg
     9481 ttagaaatca tggtagaacc ctgtaaatcg ctttttattc agcaacaggc tcttggaatt
     9541 aaattactcc gcctcttaaa ccagggagtc atcgatgcgt tacacaaact caataagcga
     9601 ttgaaataca cttaacaaaa atcataaaac aaatattgat tttgattgct ttattcagca
     9661 ctaacaattt acgcaccaag tgtagctggc cgattcattt taaaaatgta ttctagtaat
     9721 taaccccata cccatatgcc atattagata tcgcagcagc taaaccaata tgtcttggat
     9781 cagaagcagc cgttaagaca ttactttctt tgtcccatgt aatggcttgc atatctccat
     9841 aatattgttt caatggcatt aaatggtatc ccatagcttg taaaccctgt tgaattgaac
     9901 ttgggaaagc atcaggctca aattgcaaaa catctggtaa atattgatga tgaaatcgca
     9961 tggctgaaac catactgata gctccatacc catcataaaa taatagtgag gcaagtaaaa
    10021 ccatagtagg aatacggctt cctccaggag tccccaggat agctacacgc cctggcatct
    10081 ccaggaaggt tggagccatg ctggatagcg gcctttttct tggttcaatg gcattgcgct
    10141 cacttcccac taaaccaaag acattttgct cacccacttt agtagaaaaa tcgtccattt
    10201 catcatttaa taatacccct gttcccttcg caactacgct ggagccaaaa atgtaattaa
    10261 ttgtcattgt tgcagcaaca cgatttcctt ccgtatcaat aatcgaaata tgtgaggtat
    10321 ttgagtgttt tcccgtcgga actgagcctt gcaagacttt actatcaatc gctttatttg
    10381 gtgaaatcaa gcggcttaat tgtttcgcat tatccgagga aatcaattta tcaagtggaa
    10441 ccttaataaa atcaggatca cccagaaatt gatctctttg ccaataagcc aatctcatcg
    10501 attcaacgac gtaatgaacc cattttaatt tgggaaatga agacaaagga aatttggata
    10561 atatattgag catggttacc aatgccactc caccggccga aggcagagga gctgtgataa
    10621 ttaacatatt atgataagca ctaattaaag gtggcctcac tttaatttta tattgagcca
    10681 gatcatcaag agaccaaatt ccacctgccg cattgacacc ttgaactagc agagaagccg
    10741 tttctcctga ataaaatccg gcatttccgt acttggctat caatctgagt gtttttgcca
    10801 aatctttttg aacaagtttt tctccaattt caaaaggctg accattcttt aaaaaaatag
    10861 cggcggtatc gggatatttc ttcaagtttt ccaatctatc atccagagtg gagaaataac
    10921 gaaattgagg atctacagga aaaccctctt ccgcaagctt aatagctgga gctaatgtct
    10981 tgctaagcgg aagacgtcca tagttcttgg ctatataaac cagggcagct ggctgtccag
    11041 gtatggccgc agataaacca ccatttagag aaaggccggg aatcacttgg ccatcaggag
    11101 ccaaatacat atccttactt gcggccagag gagctacctc cctgccatca ataaagaggt
    11161 ttaaatgttc cttttcctga tgaagtagcc aaaaacctcc tcctccaagc cccgaatgat
    11221 atggctcgac tactgccaag gcagcagcta ccgcaactgc agcatcaaaa gcatttcctc
    11281 ctgctgccaa agtctccaaa ccggcatgag ttgccaaggg gtgagcactt gctacagcat
    11341 atccaggagg gtgttgtgga aatgtttttg cattaacctg ataaatatta caaattaaaa
    11401 aaaataaaca actcagccat ttatagttat atttcatttt gtctcttttt taattctcta
    11461 actacattgg gcggaacaaa ttgagaaata tcaccattta aggctgcaat ttcacgaact
    11521 aaagtggatg agatatacat taaatgctct gctggagtta aaaataatgt ttccaccttt
    11581 ttagatagct ttctattcat tccagccagt tgaaattcat attcaaaatc agaaacagct
    11641 cgcaatccac gtaaaataat gcctgcactt tgttctaata caaaatcaat taacaaatta
    11701 tcaaaaccca ctactctcac accagttaaa tgtccaacgg actcttccag taaactaatt
    11761 ctggtttccc aagataaata aggtcgtttg ttcttattac tcgctactgc aactatcaat
    11821 tcaggaaaaa tagtgctggc ccgagtaata atatcaatat ggccattggt tacagggtca
    11881 aatgttcccg ggtagattgc tttttgtttc atctcattaa ttaccatttg catatagaag
    11941 cagtctagcg tattgtaaat tgaaaaacta taaaaaaatg gaattgaggt aagcaatgaa
    12001 tgcgataaaa cgtttgacaa tcattttaat ttgctttcta acagcatgcg ctcccccaag
    12061 accctctgaa gagccgcctg caaaccgtga aattcctctt gaagaacgca aagccgaaac
    12121 agccactgtt tcctcatggg aaattaaagg cgccctggct gccaaaacca aagcgaaagg
    12181 ctggtctgct actatgaatt gggtacaaca aggggccaac tcataccaaa ttcgtttaat
    12241 ggggccatta ggtggtggag ccgttctaat cagcaaaaaa ggcaatacta ttacatttca
    12301 agatggttcc aaaataacta cttcgaccaa tgccgatgag ttattactaa aacaaactgg
    12361 cattcgactt ccagtaaata acttgtatta ctgggtaaga ggtttacctg ccccaggtgg
    12421 agtccaatct gaaaaacatg accaattcaa tcacctgata caattaaagc aaaatggtta
    12481 taccattgat tttactaagt atacttcagt caagggaata gatttaccaa gtatgattcg
    12541 ccttgaagga aatggtgtca tggtaaaggt agtgataaag aattgggttg tataatttaa
    12601 ttcttgtaat ttatttgctt tctgaagttg acatcgataa aaaagcactg caaaatacgc
    12661 agcacattgc agggatgaat acaatttata aactcatgga tgattaaaga atctacaatt
    12721 ataggggtgt cgccaagcgg taaggcacag ggttttgatc cctgcatacc taggttcgaa
    12781 tcctagcacc cctgccattt tcttgttgat tcaacctaga atatgaagtt gaatctaggt
    12841 acaagtaaac agacggcaga agataacctg gaaggatgcg tacttcgtat cgctcaaata
    12901 ataataatat ctttttggct gtcttctgct atgtgtttaa gttatgtgat taacctaaat
    12961 ctaaggcttt tgacacatgt ccacgatgat gatttttaca gggaatgcta accctgaatt
    13021 agcactaaaa atttcctcac atttgcaaat acctattggc aaagccaccg ttggtacctt
    13081 cagcgatgga gagactatgg ttgagatttt ggaaaatgtc cgaggtaaag atgtttttgt
    13141 cctccaatca acttgtgcgc ctgcaaataa taatttaatg gaattgctga ttatggcgga
    13201 tgccctgaga agatcatctg ctggtcgtat tacagctgta gttccttatt ttggctacgc
    13261 acgccaagat aggcgagtgc gctccgcccg tgttcctatc acagctaaag tggtagcaga
    13321 catgatggct tccgttggaa tttgtcgggt ccttactgtt gatttgcacg cggatcaaat
    13381 acagggtttc ttttatatgc cggtcgataa cgtatactca actccggtat tgctggaaga
    13441 tataaccaaa caaaaattaa acaacatcat gatcgtatcg ccagatgtcg gtggggttgt
    13501 tcgtgccaga gcagttgcta aaagactgaa tgacgctgag ctgtcgatta ttgataaacg
    13561 ccgtagtggg ccaaataaat cagaagtaat gcacattatt ggtgagcccg ccaataaaaa
    13621 ctgtattatt gtagacgaca tagtagatac tgctggaaca ctttgtacgg ctgctcagga
    13681 attaaagaaa aacggagcaa aaagcgtaag agcttacatt acacatcctg tgttgtctgg
    13741 tcccgcagtt aacaatatta aacattcagg tcttgatgag gtggtagtaa ctgataccat
    13801 tcctttatca gccgaagcac aaaactgtga aaaaatcaga gtggtcagtc ttgctgatat
    13861 gttagctcaa gcaatcaaac gagtaaatgt tgaggagtca gtaagctcta tgtttgctga
    13921 ataaaatgaa atattcattg ataataaaaa gggagccaag gctccctttt tattatcaat
    13981 gtttcttaat cccttgtacc aacgtatttg tcatcaaaat aacgtcttaa tttagttctt
    14041 aaagttccac gactaattcc cagcatgatt gcagcgcggg actgattata cttgcaatgt
    14101 tccattactg cacgaaatag aggggtctca atttcttcaa gtacaaactg gtataagtca
    14161 acaggatcag ttcccttaag ctctgaaaaa tatttttgta cggattgagt cacgctttcg
    14221 gctagtgaag cagtggtatc tcctgcatca ttactgattg ttgtcattat tattagctcc
    14281 tccttttaaa agcggtatat taccgaattg agcaatacga gacaagggca gaacagaaaa
    14341 aactgcatta aaattcaata tatactgttt gcacttgaat cattgccacg tttctcacca
    14401 cagtggattg aaaagaggat aagccgctct caacagctta tcctatattt aacgaactac
    14461 tggaatttca tcatagacgg atcaaatggt ttcccattaa ctgtgaattt tccttcactc
    14521 agactcatct caatgacata gtctttgcca tcgacagtaa ttaaaccaga ttgctccaac
    14581 tcattaattt gcttatctac ttgcattgca gcgatttgct caggagtagg ttctgcttgg
    14641 tttgattgac cttgactgct ttgcagctgt tgaattaacg cttgttgcat ttctggctgt
    14701 tttgccattt gttgcataat cgattgctgc atcagttgct tcactacctc gattggcatt
    14761 tttaacttgg cattaccttg agttttttga atcaattcaa agggattaga attatcgcct
    14821 ttgggtaaag taattaacaa gttaccatca atcatgcctt gtggaagttt gagacttaat
    14881 ttggaaattt caaactctgc acctttacta aatagctttg gcaattctgg caacattgcc
    14941 attaatgctt gttggcgctc ttgatctgtg cctctttgca tcgcattagc ttgttcattg
    15001 attttagcca gtacttcagc atcaaggttt cttaaggaca tctcgacttc accaggaccg
    15061 taattttgcc cattggcgaa taacgatttt aaagcggcac taaaatgcgt gctgaagaga
    15121 ttgtcatcta tatcgctgct tgagcttaat gtcaattcac taatttcaaa catcttttga
    15181 tctttaacaa ctacagcaag tgatggtaat gaaaaactgg cttgacccaa atataaccct
    15241 gtagtcgttt tatgtaagtc atactcgctg attaccttac ccaaagagag cttggtatca
    15301 tctttagaaa actctgcacc atcaaattca atttcccctt ccactttatc caaattggaa
    15361 gacattgatg tagtgctgtc catacccatc caattaaatt taccatttcc atctttagca
    15421 gttaatttga aagaaggaat ggacaactcc accgtgcttt tattcaaata atttacaaaa
    15481 atgctcaaat ccagctgagg cttttctgaa tcttgagaaa aaagctcatt gaattgctga
    15541 ttatattttt ctggaaatgg aaatacggtt tgtgcaaagc ccattccaaa acgtaactgc
    15601 ttattcgcgt atataatggg gccatgataa atcttaatcg gcatttccat ttgataatct
    15661 tgggcaggag tagtttgcaa ttttccatca tcgtccttta ctactcgctc aggtacgtgt
    15721 aaacgccatt taactaatgc atcagagcaa aaccaacctc tcttatattg ctgaatgtca
    15781 gcgaaaagcc cattggattg attaattacc tcgacagttt ttttaaccgt tctttcggtt
    15841 agaattccca tgccataata tccacctagg attaaaacag ccaggataat tactaatcct
    15901 gtaagttttt tcattattat ctccatgtta ttaatttgca aatagcatta ctcgagcgca
    15961 gcaaagtgct gcgaaaagta taacagcaaa taacctaaaa gtgcgacagt ctaatgcaag
    16021 aagattcaaa cttaatgaaa aatattttca ttaaatttca taaatttgat gtatttacag
    16081 caaaaatcaa acaatgatgg catatttata atgcaatgct caatgtttga tttttgctat
    16141 cttaatgtct tataggtttg caataagtct ctttaacagt aaacgcgagg attaaggcca
    16201 ccaaggaaca taaggggagt aattttagac cggcttggaa atccgataat aataacgttt
    16261 caacattata ggctctgggc ccagacacca tgtctatgat acgtcctact aaaggctgga
    16321 ataaggcccc gacaaaaatt gaagtcatat tggtaaatgc gatagatgta ccaacaacac
    16381 tcctgtcatg tatttctgta gaaacagcgt atgaaatcgc aactccagtg tttgtaagac
    16441 caaaaacaaa aaatagtatg taagccgttg tttgactcat ttcaggaata aaaacaaata
    16501 aactgctcaa gatcacacca catactgcgg aaataatcat caatggctta cgacgtccca
    16561 ttttatcaga aatccaacct gataatggac cactaattcc ccagcctata aagatcaatc
    16621 ctgttgcaaa tgcagctgcg tgcgccccta aaccacggcc gaactgtaaa tatgccggac
    16681 ctattgcctc tccaattaca gcggtggggc caaataaaaa tccagcatac atcgcattaa
    16741 gccaagtttg tttatttgat aaaatgatct ttaagctatc caaaatactg attcgattaa
    16801 cagaacgaat ttcattgcga tgctcgccgg gtttatcttg cacaaattga tacaataatc
    16861 ctgataaggc aataaataag aacgcgataa tcagcatact atggcgccag ccaacattac
    16921 tcactaaaaa tgaaacaggg gcctcccctg cagcagcacc aagcattcct aacgcttgag
    16981 ttaatccaga taataatccg agcatagttg gaggaaacca tgaagtggca aggcgaagtg
    17041 agcaaatgaa tgcaaaagcg gcactgaaac caatcaacat tcttccaatt gatgcagtca
    17101 gcagaccatc cgcaaggcca aatacacagc aaccaaaagc agtaactaat gacataatag
    17161 tcaataacca tcgtatgctt aatcgatcaa ccgtcaatcc tacaggaatt tgcattaaga
    17221 tatatggaac atagaaggat gcagttaata tgccgaagcc agcctcatta atgccaaagt
    17281 ccatttgtag atatcggtgc attacaccgg gcgctactct agctaaataa tcagaaaaat
    17341 aaaaagcagc tgccaacccc caaactaacc acgcaaaacc caggtaattt ttacgtttat
    17401 aaatttccac tttagatatt cccattttgg tataaccacc acatgctgta tttatttaaa
    17461 aaatgaccaa taaattaaca atgataactt atcattatac tgctgttgta acttatttca
    17521 acatatagtt tatttttacc tttgccctat tagtaatgta ttgaggattt aggatataat
    17581 cctaattttt aatgactcat cgtcatccag caaaacaaaa atcctctgga ttgagttgaa
    17641 tgattaaaag accaggactt acgaaaaaat ccaaggtcga ggaaaaaatg ttttaaatga
    17701 gagaatttag acaaattaaa taactgaatt aaaaatattt tttaaccaag agattggagt
    17761 tttcggatat ccagtggatg aattcaaaac aaaactgaat aatccccgag ggttcaccaa
    17821 gaagttttaa agagagtaag gcaaaatcat attttgatta tataatgaaa cattcgagtc
    17881 gttcagccta tacggatgac aaaaataggt tcaaatttat aaagcaccag ggctcataat
    17941 tgaccgaaat tggtatgccc ttgccctgtt aaaattgaat cttcatatat tttcatgaat
    18001 ggaagttggt tactctgaac aagtttaaaa agcaaatttt caagatacta tacagacatg
    18061 agatccaccc ctgatgaaca aactaaatat agccattaaa gaagcttttg aatcttccgg
    18121 aagctcaaaa gaagcgaata aagtttacct tgaatttatc aaggcaaatt ttattattcc
    18181 tattgaatcc aattctgatg atagtgaacc cagagtatta tttttggaag aaaataacca
    18241 tgcttttttg ccggtattca ctgatatgaa atacctcgat gcttgggcaa gtgaaattag
    18301 ccaacatatc aaattactta aattatcagg ggttgacctg ctgaaaggtt taggtgagaa
    18361 agttacagtt tgccttgata ttggcagtga gctttataaa gaattcaatc catctgaaat
    18421 tgaacgaatg aggtcgatgg ttttaaaact atttaaagac tgaactcaat acaaggaaca
    18481 ttcccattca attgaagaag cattacatta tgctcttcaa ttgaacgcga tgatcatgct
    18541 acgagctcaa gtaattggta gcctactatt gcggaagcgg ctatcgcacc accaatttca
    18601 aagactaaag ataggaagct gtttttatga tgatactgct gatcatttgc agctgtggtt
    18661 ttatctaaaa gaatatcttc ctcgatattg ttcaaatcaa ataatggttt ttctccatat
    18721 atgccatccc accatccttc gagccattgt tctgcttctt gagaaccttc gcggaaagga
    18781 ttatcctctt ctggaacttc tgccaatgca cattcatacc caaaaatata acattcttca
    18841 tagcttgggt gttcaatgtt aaatcgtaat ctaagatgtg ataataatga tgttgtatcg
    18901 ttcatgatga tagcctcttt tatgtatcct gaatatattt ccgcttttca actcatttac
    18961 tcctaaatct ataattatta acgcaattgg tgcagaactg ctttttagtg catgtaactc
    19021 ccacacttaa atcaatttta cgactttaat ttgccccctg ccttcgaaga tcaaatattt
    19081 acattatctt gacaaaaatc tttttacctt tggttcttta acacgatact tcattaactt
    19141 attgatttta ttaatagaga tgcaagtatt aggccaactt ttttatctta ttttttcatg
    19201 gactttt
//
LOCUS       DATNSN010000049        19106 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.049, whole genome shotgun sequence.
ACCESSION   DATNSN010000049 DATNSN010000000
VERSION     DATNSN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.049"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            82..753
                     /locus_tag="VAH76_001418"
     CDS             82..753
                     /locus_tag="VAH76_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEO1386050.1"
                     /translation="MRLLIVEDHKRTADFICKGFNEYQFQADAVYDGAEAIYRISEIN
                     YDAVVLDVMLPIMDGWAVLEKIRELKPDLPVLMLSACDDVDSRVKGLEMGAYDYLVKP
                     FSFNELLARVKSLLRRKPKESPILLKINDLTLNLQKHTAIRGDKKLPLTAKEFMLLTF
                     MLKRSGEVLSRTVIAEQVWDINFDTNTNIIDVAIKRLREKVDNDHPTKLIHTVRGVGY
                     VLEIR"
     gene            734..1831
                     /locus_tag="VAH76_001419"
     CDS             734..1831
                     /locus_tag="VAH76_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEO1386051.1"
                     /translation="MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHH
                     SHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINA
                     SSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
                     TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHL
                     ISSIIEYYYSIAQEKGIEIQMFGEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILII
                     IEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIME
                     HHKGQLAIDSKMGLGTTVILTLPGKSFLKIE"
     gene            complement(1973..3973)
                     /locus_tag="VAH76_001420"
     CDS             complement(1973..3973)
                     /locus_tag="VAH76_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEO1386052.1"
                     /translation="MSDTPFIAANKKVAELTFRVIILAIILTVLLAMSNAYLALKLGI
                     LTSASIPAAIISMGILRFFKNSTILENNAVQTAASAGEAVAGGIVYTIPALIIIGFWN
                     HFDYVTNFFIAVCGGILGVLFSIPLRRILVNDQTLKFPEGRAIAEVLKSSADKVGIKD
                     IFLGGLVGGLLELLQIGFKVIASSWNYWFVVKRSLFGLGAGFSATMIGAGYLVGHDMA
                     ISIFLGAVISWLIALPIASQFYPDFLNQYPAEQAATLLWNSEMRYLGIGAMLFAGIWT
                     FIKLAKPLTRSVKTSFRTYISKSKFREQYPRTEKDIPMPFILMGIGLMAGILFLFFQI
                     IFPLGQVGLDNDFSPTIVFIGVIYVLVVGFLFSVITAYFSGMVGVTASPGSSVVISGM
                     LFAAWLLVSAINHLLPLPLTSHQIQAAEAITIIIGAVVTGIAAIANDNTQDLKVGQLV
                     GATPWKQEVMLLLGVVISALVIPPVMQILFNVYGIAGVMPHAGMDVTQSLPAPTAALM
                     AAITEAVFRNSLPWNMMLAGSCIILLAIFLNRILNIKRFLNLSILGIAIGMYLPLSSS
                     VPLFIGGMIALFVQNRLGRLKVNPEESTNRKQKGTLIACGLVAGSAIMDVLLAIPFSI
                     FHSPDALQLVGSSWKQYGVYLGVFSTLLLAWWISHRVCRSKI"
     gene            complement(3973..4668)
                     /locus_tag="VAH76_001421"
     CDS             complement(3973..4668)
                     /locus_tag="VAH76_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HEO1386053.1"
                     /translation="MPIQMNLINKIGLMFGLLFYSFLASAITTEVIKKETAEYLLDIK
                     YPQGFQSNGVNQAVKEFITNQQKSFMNELSEDADTPADAPGKTGLNITYAIPYQSTNA
                     LSVRFNISIYHRGAAHPSNAVKVLNFIKNQPVQLSDLFVPGSDYLQPIADLAKKEITA
                     KKISDENWIKEGTKPTQENYSIWHFTKKGIAIVFNTYQVAAYVYGEQTVDIPLSLISA
                     MVKPEISKAVWGN"
     gene            complement(4695..5906)
                     /locus_tag="VAH76_001422"
     CDS             complement(4695..5906)
                     /locus_tag="VAH76_001422"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEO1386054.1"
                     /translation="MFACDELLLNASTIDATGLQLSNQAIVIKKGRIEWCGSEDQLPA
                     HFQESAKSRKDCHGQLITPGLIDCHTHLVYAGHRAAEFRLKLQGVSYADIAKSGGGIL
                     STVQMTRDASEEELIDQSLPRLLALKNEGVTTVEIKSGYGLDLQNELKMLKVARQLGE
                     MAGVRVKTTFLGAHAVGPEFKGNSQAYVDFLCNEMLPAAKNMDLVDTVDVFCESIAFS
                     IRQAEQIFQAAKDLNLPIKCHAEQLSNMGASSLAARYGALSCDHLEFLDENGALNMVK
                     ANTVAVLLPGAFYFLKEKQKPPVDLLRQVGVGMAIATDSNPGSSPTTSLLLMMSMACQ
                     FFSMSIPEVLSAVTYQASRALGMEKDIGSIEAGKIADLVLWSIKDSAALCYYFAYPLP
                     HQTMVAGEWVS"
     gene            complement(5906..6715)
                     /locus_tag="VAH76_001423"
     CDS             complement(5906..6715)
                     /locus_tag="VAH76_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1386055.1"
                     /translation="MSGIISPIVFYIDEEIVYLITGGGSGIGRALAFALAKRNKQVFI
                     VGRREKLLQETASLSANIHYLCADVSTPQGRELIKASLSTTPQIDGLINNAGTLEPTL
                     LLKDISFAEWHQALNTNIDAALFLPQLLYDKLSGGRVLNVGSAAAYFPIKGWGAYCVS
                     KAALAMLTRCWQIECDSLAFASVMPGIIDTHMQAIARNGINLDPEQVNFYRRLKDHNR
                     LISPETVAEFFIWLLLDIDNETYVSKEWDIYDTTHHSAWLKPPHQVLHWEF"
     gene            7006..7965
                     /locus_tag="VAH76_001424"
     CDS             7006..7965
                     /locus_tag="VAH76_001424"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEO1386056.1"
                     /translation="MFDDLSNYRPANPALWQGRKDTANQERFFQKITFIDNQNELMTK
                     DKKTIFLGFASDAGIKRNLGRTGAKLGPDQIKTQLAKLPCHNNKHYVDLGNVVCENDE
                     LELAQSQFAQIVHFCHENGHQICAFGGGHEIAWAHYQGLSSLYPKLGVINFDAHFDLR
                     PYKKGEFGNSGTPFSQIATYCEEKKMPFHYCCIGVQKFGNTPSLFEKAKKLNVSYLSA
                     EDLYEQSQAWQIAFLDDFILNLDHIYLTICLDVLAECYAPGVSAPQALGLSPWQIMPL
                     LKYLIQSGKVVSLDIAELSPPLDSELKTARLAALIIAELLDTN"
     gene            7978..8952
                     /locus_tag="VAH76_001425"
     CDS             7978..8952
                     /locus_tag="VAH76_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEO1386057.1"
                     /translation="MKKTVLWGALFTLITTQANSEATQQAISTQNSASTGTNVVSEST
                     NQVPATNQNTQQNQLNQPVQSNGQQPTQTIQNNQQNQPSQTGEQQSNQNTQNNQQNQP
                     AQQQTTQQPAQQQVPSQQTAPVINCDYKISAETKAIEQSLVLTWSEKAIIQSFDFDPA
                     TVDAQLQKLQACFTDQGWLSFNSALQKSGNLDAIKSQKLHVSSQIDGQPQVTEAIENQ
                     WKIIIPLQVVYQNDKEKVTQLLNVNVTVGRKITGDLGINQMIAVPRTATNTSQTNSPS
                     NTNPADSNTSTSQTPGDTTNNATQTNTQTQTGTPTNSPPSGSTTNTQN"
     gene            9009..10181
                     /locus_tag="VAH76_001426"
     CDS             9009..10181
                     /locus_tag="VAH76_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEO1386058.1"
                     /translation="MRLIFTGVLCLLTLNAQANVIQYFAGISYNNPADLFKVKNGILL
                     VGGTGSYADLQFKGSALNFNTFQYDSGVNHSRTYIVWPYGRVAKRLNDKTVVAVDLTE
                     PFNSNLDWGNDAFTRYAATQNYLTDVDLSPKISYAISKKLQIGGGINFNVLLKNEVNW
                     AFPTGQSTYANLINRSSSFGVGYNLGINYAVNDTNFLGITYYSRIRQNTSGTSYLGLA
                     ANPDFQFGFYMPATTVASYVHIFNPKWLINLQVFQSEWNANQKVRLYNTAAPPPFTNF
                     IFDMHFDASYAYLAAIRKQVSDKLGIALAGMIDDGPEEDGLRTIVFPSDTQYFLGLIG
                     DYRFTEHASMELILGHVYSNPSIQNKAKVNNVPVPFTTGRVTINANVLDLKVKIEG"
     gene            complement(10221..10646)
                     /locus_tag="VAH76_001427"
     CDS             complement(10221..10646)
                     /locus_tag="VAH76_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO1386059.1"
                     /translation="MYNIMSFLCGLLFGAGLTISNMINPNKVLNFLDINGFWDPSLLI
                     VMAVAVTVTFLGYKLINKFDKPILCNQFYLPEKKNIEKKLILGSIIFGIGWGVAGYCP
                     GPSITALTTFNSDPVYFVIGLIAGSWTYYWLFKKRTIQK"
     gene            complement(10646..11083)
                     /locus_tag="VAH76_001428"
     CDS             complement(10646..11083)
                     /locus_tag="VAH76_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEO1386060.1"
                     /translation="MDYITSFTPYLSAIGGLLIGLSAVLFLWLNGRIAGISGMVHGLF
                     PPEKPFPVWRIAFLLGLAAAGLIYFISPVIQFPLRTHYPVFLLLIGGFLVGFGTRMGQ
                     GCTSGHGVCGLARFSKRSFIATLLFILSAMITVYLIRHVGGLY"
     gene            complement(11193..11804)
                     /locus_tag="VAH76_001429"
     CDS             complement(11193..11804)
                     /locus_tag="VAH76_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1386061.1"
                     /translation="MKKIYWAVILMCLGLTSCVEFDESTLITDDAGYVHRTVHYLRDK
                     RGRNYFPMQIKAPGERLFVFDPKASAWAAYDEEGHRVMTGAGSGGIDVCEENPNQSCR
                     TITGTFRIYNKRGIDCRSGEYPVDTKGGAKMPYCMYFYRGFTIHAAYEVPEHPSSHGC
                     VRVFPSAAKWLNEEFLRVGTKVIVLAYPDQNGVKTMSSLEKVN"
     gene            complement(11846..13519)
                     /gene="ettA"
                     /locus_tag="VAH76_001430"
     CDS             complement(11846..13519)
                     /gene="ettA"
                     /locus_tag="VAH76_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEO1386062.1"
                     /translation="MAQYIFTMNRVSKIVENQRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDTQYEGEARPQPGIKIGYLAQEPELDLSKTVREVVEEGVAEIKEKLS
                     RFDAISMRFAEPMSDDEMNALLMEQGELQNEIEACGGWDLERKLDVAADALRLPEWDA
                     IIGKLSGGERRRVALCRLLLSSPDMLLLDEPTNHLDAESVAWLEHYLEEFSGTVVAIT
                     HDRYFLDNAAEWILELDRGEGIPYKGNYSSWLEQKEARLSMEKKQEDALQRAIKAELE
                     WVRTSPKGRHAKNKARLARFEEMNSKEFQKRNETNEIYIPPGERLGDLVLEGEKICKS
                     YGDRILIDNFDFKLPKGGILGIIGPNGAGKSTFLKMLTGQEEPDKGVIRIGETVKLAY
                     VDQMRDNLNPNNSVWQEISDGHDIMQIGNFQMPSRAYVGRFNFKGTDQQKKISQLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPSNDLDVETLRALEEAILNFPGCAIVISHDRWFLDR
                     ICTHLMAFEGDSQITFFEGNYTEYETDRKRRLGDEANRPSRIKYRKLSD"
     gene            complement(13562..14584)
                     /gene="tdh"
                     /locus_tag="VAH76_001431"
     CDS             complement(13562..14584)
                     /gene="tdh"
                     /locus_tag="VAH76_001431"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEO1386063.1"
                     /translation="MKSLVKAKKEPGIWMQDVPVPEYGVNDVLIKIKRTAICGTDIHI
                     YSWDEWAQATIPVPMTVGHEFYGEIVEVGKEVQGLKVGQRVSGEGHITCGFCRNCRAG
                     KRHLCRNTLGVGVNRPGCFAEYLALPATNVIALPDNITEEQAAILDPFGNAAHCALAF
                     DVVGEDVLITGAGPIGIMAAAIVRHIGARHVVITDVNDHRLELARQMGVSRAVNVKYQ
                     KLSDVANELGMLEGFDVGLEMSGNPMALNDMMKAMNHGGHVALLGIPPQETPIDWNQV
                     IFKGLVIKGIYGREMFETWYKMIAMLQSGLNISPVITHNFPVDEYQHAFQIMASGQSG
                     KVILNW"
     gene            complement(14673..15857)
                     /locus_tag="VAH76_001432"
     CDS             complement(14673..15857)
                     /locus_tag="VAH76_001432"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEO1386064.1"
                     /translation="MLERFINYLQNELETLKADGLYKSERVISSQQQADVKVNEREVI
                     NLCANNYLGLANDPELIAEGQAALAKYGYGMASVRFICGTQTPHKQLEQKISHFLNKE
                     DTILYSSCFDANTGLFETLLTEEDAIISDALNHASIIDGVRLCKAARYRYANNDMKAL
                     EEQLIAAKNARFRLIATDGVFSMDGILANLPAICELADKYDAMVMVDDSHAVGFMGKT
                     GRGTPEHFGVSDRIDIVTGTLGKALGGASGGYTASNQVVIDWLRQRSRPYLFSNTLAP
                     VIAHTSCVVLDKLSTNNSLAEKLKRNSHYFREGMTQLGFELIPGEHPIIPVMLGDASL
                     AGRIANRLLELGVYVVGFSYPVVPKGLARIRTQMSAALELHHLDKALKAFETVGKEFS
                     VI"
     gene            16038..16688
                     /locus_tag="VAH76_001433"
     CDS             16038..16688
                     /locus_tag="VAH76_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEO1386065.1"
                     /translation="MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHF
                     AYSDMQIPLAHGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVI
                     SIDYYSEFTTNAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHK
                     LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF"
     gene            16700..18067
                     /locus_tag="VAH76_001434"
     CDS             16700..18067
                     /locus_tag="VAH76_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEO1386066.1"
                     /translation="MRKSLFCWILTLGVSTHAFATDLMDIYQQALENDTIFKEAYDTY
                     MSSTEAIPQARAALYPQVGLGSQAGRNYQDAVAGAFSANQYYGSYLWQVKASQALFNY
                     QAWAQVAQAKASVKAAQASFNDAAQNLILRTAKAYFDVLFAKDTLDFAEAKKRANKRQ
                     YDQATQRFQVGLDAITSVYEAKAAYDQSIATVIAARNNQINQSENLRKLTNHVYETLA
                     PLKDSKIPLVKPEPNDVNQWIDTGLKQNYKLYAAKYNLEVAKENVKAISAGNWPVFSL
                     QSNASQVHNNASGNNVFIPSKQTQANIAIAMNFPVFQGGLVQAQTRQAQYNFQSTSEK
                     LEQTYRDVIVNSRIAFNTITDGISKVKADRQTVISVQNSLQSTEAQFEVGTRTMVDVV
                     NAQQRLFEAQEQLARDQYDLINSILTLKYLAGTLNVNDLEQINSWLATTRVNGFSPVD
                     NKTSK"
     gene            18085..18936
                     /gene="ttcA"
                     /locus_tag="VAH76_001435"
     CDS             18085..18936
                     /gene="ttcA"
                     /locus_tag="VAH76_001435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEO1386067.1"
                     /translation="MSSNPSSVEKKLLHYTGKAIADFNMIRRGDRVMVCLSGGKDSFT
                     LLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVK
                     EKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
                     MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLI
                     DQLAIENPKVPSNMLHALQSLKPSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEF
                     EFEDEKI"
BASE COUNT         6153 a         4132 c         3376 g         5445 t
ORIGIN      
        1 attacaaaat tgtaatctaa ttcattatga taattagcat ttttatgtta aaatagaaca
       61 taatttaaac acaatagcat tatgcgatta ttaattgttg aagaccataa aagaacggct
      121 gattttatct gtaaaggatt taatgaatat caatttcagg cagatgctgt atacgatggt
      181 gcagaagcaa tctatcgaat ctcagaaatt aactacgatg ctgttgtttt agacgttatg
      241 ttacccataa tggatggatg ggctgtactt gaaaagattc gtgaactaaa acctgattta
      301 cctgttttaa tgctttctgc ctgcgacgat gtcgatagtc gtgtgaaagg gcttgaaatg
      361 ggtgcctatg attatcttgt taaacctttt tccttcaatg agctccttgc ccgagtaaaa
      421 tctttactac gaagaaaacc caaagagagc cctatcttat taaagattaa tgatttaact
      481 ctaaacttac aaaagcatac agcaatacga ggcgacaaaa agttaccttt aacagcaaaa
      541 gaattcatgt tattgacgtt tatgttgaaa cgaagtggtg aagtgctttc aaggacagtc
      601 attgctgaac aagtttggga tattaatttt gacacgaata ccaatatcat tgatgtagcg
      661 atcaagcgtt taagggaaaa agtggataat gatcatccta ctaaattgat ccataccgtc
      721 agaggagtag gctatgtatt ggagattcgc taactcaatg tccattacta accgcttatt
      781 actgatattt attggttcaa ccgtactgat attgtccatt atcactgggt taatctatcc
      841 tccaatgaaa gaattactcc atcatagtca tttgaataat gagcattata attatttatt
      901 aacacggatt tgtattaaaa atttctttgt tggactctgg ttttctactt tgataatcat
      961 tcttgccagt tatttgctcg caaaaaaaag catgagacca attaaaatat tcactaaaga
     1021 gcttgcatcg attaatgcct catcactaga gaaacgatta ccttacacag ggaatcccaa
     1081 agaactggaa gaattagctt tgacttgtaa tgatatgctc tttagaatag aaagtgcatt
     1141 tagccatata aagcaatttt cagccagtat agctcatgaa ttacgaaatc ccattcatta
     1201 cctccgaact gctaccgaaa ttaccctgac taaaccacaa acgattgaaa cctaccaaca
     1261 tgtattacaa acgcatctcg aagaatatca aagtttaacc caacttatag ataatctgct
     1321 ctttttaact cgctgtgagc acggccaact tcaattaaat gttagaacat tatctgcaaa
     1381 ccacttaatc agctcaatta tcgagtatta ttattcaata gctcaagaaa agggcataga
     1441 aattcagatg tttggcgagg ctcagattga agtagatgaa catttattta agcgcgttat
     1501 ttcaaattta atagacaaca gtctgaccta taccaacaag ggcggaagaa tactaatcat
     1561 catagaaaaa agaacagaca acagcattca aatccgtata aaagacaatg gaattggtat
     1621 atcaaaagaa cacttacctc ttttatatca cggattttat cgcgtagatt atagcactaa
     1681 caaagaaaac gcaggtcttg gtttaggttt ggctatcgct aaatcaatca tggaacacca
     1741 taaaggccag cttgcgatag atagcaagat gggtttaggc accactgtta tattaaccct
     1801 accaggaaaa agctttttaa agattgaata atgtatgtat ttcatctatc cgagacctta
     1861 agggaacgga tatacattga actaagttat tctaagaaca tagacttttc ccgcctcaca
     1921 cagcaacacc aatctacaat aagtagaatt gttagagtaa ttattaatta actcaaattt
     1981 tgcttctgca gacacgatga cttatccacc atgctaaaag caaagtagaa aatactccta
     2041 aatacactcc atattgtttc catgatgatc ctaccagctg caaagcatca gggctatgaa
     2101 aaatagaaaa cggtatagcc agtaacacat ccatgatcgc tgatcctgcc actaaaccac
     2161 aggcaataag agtgcctttt tgctttctat tggtagattc ttcagggtta actttcaacc
     2221 gtcctaaacg attttgaaca aatagggcaa tcattcctcc aataaataaa ggaaccgaag
     2281 aggaaagagg taaatacatt ccaatggcaa tacctaaaat agataaatta agaaagcgtt
     2341 tgatgtttaa tatgcgattg agaaaaatag ccaataaaat aatacatgaa ccagccaaca
     2401 tcatattcca gggcaatgaa tttctaaata ctgcttccgt gatggctgcc attaatgcgg
     2461 cggtcggggc gggtaacgat tgtgttacat ccattccagc atgtggcatt actcctgcta
     2521 tgccatagac attaaataaa atctgcatga ctggaggaat aaccaaagca gaaataacaa
     2581 cgccaagcag caacatcacc tcctgtttcc atggagtagc cccaactaat tgacctactt
     2641 ttaagtcttg ggtgttgtcg ttagcgatag ccgctatgcc tgtgactaca gcacctataa
     2701 taatagtaat agcttctgca gcttgaattt gatgtgaagt taatggaagg ggcaatagat
     2761 gattaattgc tgaaaccaat aaccatgcgg caaaaagcat acccgagata accactgaac
     2821 tcccgggact tgcagtgact cctaccattc ctgaaaaata agctgtaatc actgagaata
     2881 aaaaaccgac gactaaaaca taaataacac caataaatac aatagttggt gaaaaatcat
     2941 tatccagtcc aacctgccct aatggaaaaa tgatttgaaa gaacaaaaac agtattcctg
     3001 ccatcagtcc aattcccatc aatataaatg gcatgggaat atctttttct gtgcgaggat
     3061 attgttctct gaacttgctc ttggaaatat aagttctgaa tgatgtcttg acacttctgg
     3121 tgagtggttt cgctaatttt ataaaagtcc aaataccggc aaacagcatg gctcctatcc
     3181 ccaaatagcg catttcacta ttccataaca aagtagctgc ttgttcggcg ggatattgat
     3241 ttaaaaaatc aggataaaac tggctggcta ttggcaaagc gatcaaccat gaaatgaccg
     3301 ccccgagaaa aatgcttatt gccatatcgt gaccaacgag gtagcccgca cctatcatgg
     3361 ttgcagagaa gccagcaccc aaaccaaata gagaacgttt tacaacaaac caataattcc
     3421 agctgctggc gattactttg aaaccaattt gcagtaactc gagaagtccg cctacaagcc
     3481 cgccgagaaa aatgtcttta ataccaacct tgtctgccga agatttcaag acctctgcaa
     3541 tcgctcttcc ttcaggaaat ttgagggttt ggtcattaac aagaattcga cgcaaaggaa
     3601 tagaaaacaa aactcccagg attcctccac aaacagcaat aaaaaaatta gtgacgtaat
     3661 caaagtggtt ccaaaacccg atgataatga gcgcaggaat agtgtaaacg atacctcctg
     3721 caacagcctc accagcagat gctgctgttt gtacggcatt gttttcaaga atggttgaat
     3781 ttttaaagaa tcgcaaaatc cccatggaaa taatagcggc gggaattgaa gcggaagtta
     3841 atataccgag tttaagcgcc agataagcat tagacatggc cagtagaacg gtcaaaatta
     3901 tcgccagaat aatcactcta aaagtgagtt cagctacttt tttattcgct gcaataaatg
     3961 gagtatcaga catcaattac cccacactgc tttggaaatt tctggtttta ccatagcaga
     4021 aattagtgat aatgggatat ccactgtttg ctctccataa acataagctg ccacctggta
     4081 tgtattaaaa acaatagcaa tccctttttt ggtaaaatgc caaatactat aattttcctg
     4141 tgtcggtttc gttccttctt taatccagtt ttcatctgaa atcttcttgg ctgtgatttc
     4201 tttttttgcc agatcagcga ttggctgcaa ataatcggaa cctggaacaa ataaatcaga
     4261 caattgtaca ggctgattct tgataaagtt gagtaccttt actgcatttg agggatgagc
     4321 agctcctcga tgatatatgg aaatattaaa tcgaacactt agggcattag ttgattggta
     4381 aggaatagca tatgtaatat ttaaaccggt tttacctgga gcatctgcag gagtatcagc
     4441 atcttcagat aattcattca taaaactctt ttgctgatta gtaataaatt cctttaccgc
     4501 ctgatttact ccgttacttt gaaatccctg agggtatttt atatccagca aatactccgc
     4561 tgtttctttt ttaataactt ctgttgtaat cgcagaagca agaaacgaat aaaataatag
     4621 cccaaacatc aggccaattt tattgattaa attcatttgt atcggcattg tttatctcca
     4681 atcaagtaat cctctcagga tacccattca ccagccacca tcgtttgatg aggtaacgga
     4741 taagcgaaat aataacatag tgcagcgcta tcttttatag accataaaac caaatcagca
     4801 atcttgcctg cttcaatgct cccaatatcc ttttccattc ctaaagctct ggaagcctga
     4861 tacgttactg cagataaaac ttcaggtata gacatagaga aaaattggca agccatactc
     4921 atcatcaata acaaagaagt cgttggagaa gaacccgggt tagaatccgt ggcaatggcc
     4981 ataccaacac caacctgacg caataaatca acaggtggtt tttgtttttc tttaaggaaa
     5041 taaaaagcgc caggaagtaa gacagcaacc gtattggctt ttaccatatt taacgcgcca
     5101 ttttcatcca aaaactccag atgatcacag gataacgcac cataacgagc agccaaagaa
     5161 ctggccccca tattagataa ttgttcagca tggcatttta ttggtaaatt taaatcctta
     5221 gcagcttgaa aaatttgctc agcctgcctt atagaaaaag ctatggattc acaaaaaaca
     5281 tccacggtat ctaccaaatc catattttta gctgcaggca acatttcatt acaaagaaaa
     5341 tcgacataag cctgactatt tcctttaaac tcgggaccta cagcatgcgc ccccagaaaa
     5401 gttgttttta ccctgactcc agccatttcc cctaactgcc tggcaacctt gagcattttc
     5461 agctcatttt gtaaatcaag gccgtagcct gacttaattt caacagtagt aaccccttca
     5521 ttttttagcg caagaagtct tggcaatgat tgatcaatta actcttcttc ggaggcatct
     5581 cgtgtcattt gtactgtgga taatatgccc ccgcctgatt tggcgatatc agcgtaactg
     5641 acgccttgca attttaatcg aaactctgcg gctctgtggc ctgcataaac cagatgagta
     5701 tgacaatcaa ttaatcctgg tgtaattaat tggccgtgac aatcctttct tgacttggca
     5761 ctttcctgaa aatgggcagg taactggtcc tcagaaccac accactctat tctgcctttt
     5821 tttataacaa tggcttgatt agaaagttgc aaccctgtgg catcgattgt gcttgcattg
     5881 agcaataatt catcacaggc aaacattaaa attcccaatg aagtacctgg tgaggcggct
     5941 tcaaccaggc agagtggtga gtcgtgtcat atatatccca ctctttggat acatacgttt
     6001 cattatcaat atctaacagc agccaaataa aaaattctgc tactgtttcg ggtgatatca
     6061 aacgattatg atcttttaaa cgcctataaa aattgacctg ttccggatca agattaatcc
     6121 cgtttcgggc tattgcctgc atatgagtgt cgataattcc aggcattacg ctggcaaagg
     6181 ccagtgaatc gcactcaatc tgccagcatc gtgttaacat agccaaggcc gctttcgata
     6241 cacaataagc tccccatcct ttgataggaa aataagcggc agctgaacct acattcaata
     6301 ctctgccacc tgacaattta tcatacaaaa gttgtggaag aaaaagtgcc gcatctatat
     6361 tggtgtttaa tgcctggtgc cactcggcaa aagaaatatc ttttaaaagc agtgtaggct
     6421 ccagagttcc tgcattgttt atcaaaccat caatttgagg tgtggtactt aaactggctt
     6481 tgattaactc tctgccttga ggagtggaga catcggcaca taaataatga atgtttgcag
     6541 acaaagaagc ggtttcttgc agtaattttt ccctgcgccc cactatgaac acctgtttat
     6601 tgcgttttgc caaagcaaac gccaaagctc ttcctatgcc gctaccacca ccagtaatga
     6661 gatacacaat ttcctcatca atatagaaaa caataggtga aataatacca gacattagac
     6721 ttcttgcata acctgcacgt tttattttag aacaagaaaa aagcgactga acggtctaag
     6781 tttattaaaa gtaaatgaaa atcttgagta tttagcattt ctcttggaaa ctagccctat
     6841 cattagagct cttgcataac ctgattattt tgttttaggg caaggcgcaa acgttttgag
     6901 gagcggagtt tacatgcagt aaattagcac cacagaaacg tttgcaacgc ggcaataaca
     6961 ctaaagaaac aggttatgca agaggtctat taaaaggaga acttgatgtt tgacgattta
     7021 agtaactatc ggccagccaa ccctgcgctt tggcaaggca ggaaggacac cgccaaccaa
     7081 gaacgttttt ttcaaaaaat tacttttatt gataatcaaa atgaattaat gactaaagac
     7141 aaaaaaacca tatttcttgg atttgccagc gatgccggaa ttaaacgcaa tttaggcaga
     7201 acaggagcaa aattaggccc tgatcaaatt aaaacccaac ttgccaaatt accatgccat
     7261 aataataaac attatgttga tcttggaaac gtggtatgtg aaaacgatga attggagtta
     7321 gcccaatctc aatttgctca aatagttcat ttttgccatg aaaatggcca tcagatctgt
     7381 gctttcggcg gtgggcatga aatagcctgg gcgcactatc aaggattatc ctcactctat
     7441 ccgaagttag gggttatcaa ctttgatgct cattttgatt tacgccctta taaaaaaggt
     7501 gaatttggca actcgggaac tcctttctct caaattgcca cctattgcga agaaaaaaaa
     7561 atgccttttc attattgttg tattggtgtg caaaaatttg gcaacacccc atctcttttt
     7621 gaaaaagcaa aaaaattaaa tgtgagctat ctctcagcag aagaccttta tgagcaaagc
     7681 caggcttggc aaattgcatt tcttgatgat tttatcctta atctggacca tatttactta
     7741 acgatatgcc tggatgttct cgccgaatgc tatgcccctg gtgtaagcgc cccgcaagca
     7801 ttaggtttat ctccctggca aataatgcca ctgttgaaat accttataca aagtggcaaa
     7861 gtagtaagtt tagatattgc agaactttcg ccgccactag attccgaatt aaaaactgct
     7921 agactagcgg cacttattat cgcagaattg ttagatacta attgaggagt atatttcatg
     7981 aagaaaactg tgctgtgggg tgctttattt actctaatca ctactcaagc gaactctgaa
     8041 gcaactcagc aagcgatatc aactcaaaat tcggcgtcca ccggcactaa tgtagtttct
     8101 gagtcaacga atcaggtacc tgccacgaat caaaacacgc agcaaaatca gctgaaccaa
     8161 cctgtacaat caaatggaca acaaccaact caaaccattc aaaacaatca acaaaatcaa
     8221 ccgagccaga caggggaaca acagtctaac caaaacaccc agaataatca gcagaatcaa
     8281 ccagcacagc aacaaaccac acagcagcct gctcaacaac aagtaccttc acaacaaaca
     8341 gcgccagtta ttaattgtga ttacaagatt tccgccgaaa caaaagctat agagcaatcc
     8401 ttggttttaa cctggtcaga gaaagcaata attcagtcat ttgattttga tccggctact
     8461 gtagatgccc aattgcaaaa attacaagcc tgctttacag atcaaggatg gctaagcttt
     8521 aattcagcat tgcaaaaatc aggtaatcta gatgccatta aatcacaaaa gttgcatgtt
     8581 agcagccaaa ttgatggtca gccacaagtg actgaagcga tagaaaatca atggaaaatt
     8641 atcataccat tacaagttgt ctatcaaaat gacaaggaga aggtaaccca actacttaat
     8701 gtcaacgtaa ctgttgggcg aaaaattacc ggtgatttag gcatcaacca aatgattgct
     8761 gtaccccgca cagcaaccaa tacctcacaa accaattcac cgtctaatac aaacccagca
     8821 gactcaaata catcgacctc tcaaacacct ggtgacacaa caaataatgc tacccaaacc
     8881 aatacacaaa cacagactgg gacaccaacc aactcacctc cgagtggctc tacaacaaat
     8941 acccagaatt aaattcaaca attagtgcgg cattccatta agaatgctgc caaaatcacg
     9001 gaaatatcat gcgattaata tttacaggag tattatgcct gttgactctc aatgcccagg
     9061 cgaatgtgat acagtatttt gcaggaatta gttataacaa ccccgctgat ttattcaaag
     9121 taaaaaatgg tatcttgctt gttggaggaa ccggctctta tgccgatttg caatttaagg
     9181 gaagcgcctt aaatttcaat acgtttcaat acgattcagg ggttaatcac tcgcgtactt
     9241 atattgtatg gccatatggt cgagttgcca agcgcctcaa tgacaagact gtagtagcag
     9301 tagatcttac cgaacctttt aattccaatc ttgattgggg caacgacgct tttacaagat
     9361 atgctgcaac ccaaaattac ctgaccgatg tggatctaag ccccaaaata tcctatgcga
     9421 ttagcaaaaa attacaaatt ggtggtggta taaactttaa tgtactacta aagaatgaag
     9481 tcaattgggc ttttcctacc ggccaatcca cttatgcgaa tttaattaac cgatcctcta
     9541 gttttggtgt gggctataat ttggggatta attatgcagt gaatgatacc aatttccttg
     9601 gtatcactta ttattcaaga attcgccaaa atacttccgg taccagttat ctgggattag
     9661 ctgccaaccc tgatttccaa ttcggttttt atatgccagc cacaacggtt gccagttatg
     9721 tccatatttt taatcccaaa tggctcatca acctacaggt ttttcaaagc gaatggaacg
     9781 ccaatcaaaa agtgagactt tataacacag cagcaccacc tccatttact aattttattt
     9841 ttgatatgca ttttgatgcc tcttatgctt atcttgccgc aatacgtaaa caagtctctg
     9901 ataaattagg cattgcgctg gcaggcatga tcgatgatgg tcctgaagaa gatggcctta
     9961 gaaccatcgt tttcccttct gatacccaat attttcttgg attgattggt gactaccgtt
    10021 ttacggaaca tgcttccatg gaacttatct tgggacatgt gtattctaat cccagcattc
    10081 aaaacaaagc caaggtaaac aatgtacccg ttccctttac tacaggccgg gtgacaatta
    10141 atgccaatgt tttggatctt aaagtcaaaa tcgaggggtg atcttccagg tgtaaccacc
    10201 tggcacttcc tccaagagtt ttatttttga atagtcctct ttttaaatag ccaataataa
    10261 gtccatgatc ctgctatcag cccaatcaca aaatataccg gatcggaatt aaaggtagtc
    10321 aaggcagtaa tagatgggcc tgggcagtat ccagccacac cccaaccaat tccaaaaatg
    10381 atacttccca agattaattt tttctcaatg tttttcttct cgggtaaata aaattgatta
    10441 cacaatattg gtttatcaaa tttgtttatc agtttgtacc ccaaaaaagt tacggtgaca
    10501 gcaacagcca ttacaatcag taaactcgga tcccaaaatc cattgatatc aagaaaattt
    10561 aataccttgt taggattgat catatttgaa atagtcagcc cggctccaaa taacaaacca
    10621 catagaaatg acatgatgtt atacattaat ataaccctcc tacatgtctt atcagataca
    10681 cagtaatcat tgcagataag ataaaaagca gtgtcgctat aaaggagcgc ttggaaaatc
    10741 tggccagtcc acataccccg tgtcccgaag tacaaccttg gcccattcgg gttccaaaac
    10801 ccaccaaaaa tccaccaatt agtaacagga aaaccggata atgtgttctt aatggaaact
    10861 ggataacagg gcttatgaaa tagatcaatc ctgcagctgc caaaccaagt aaaaaggcaa
    10921 tacgccagac tgggaatggt ttttcaggtg gaaagagtcc gtgtaccatt ccactgatgc
    10981 ctgctatacg gccgtttaac cataaaaaaa ggacagcact taagccaatc agtaatccac
    11041 caatggcact tagataagga gtaaaagagg ttatataatc cattatttac atttccgttt
    11101 catttacaat gaataacacc tgaattgaga cactttaaaa aattaaagtg tctctcaaat
    11161 tacattgatg ttttaaaaca atagtccaaa ttttaattca ctttttccaa tgaggacatg
    11221 gttttcaccc cgttttgatc agggtaggct aatacgatta ccttcgttcc aactcgcaag
    11281 aactcttcgt tcaaccattt ggcagcacta gggaaaaccc ttacacatcc atgacttgat
    11341 gggtgctctg gaacttcata agccgcgtga atagtgaatc ccctgtaaaa atacatgcaa
    11401 tagggcattt tagccccgcc tttcgtatcg acagggtatt cacccgaacg acagtcaata
    11461 cctcgtttgt tataaatcct gaatgttccg gttatcgtcc tgcaagattg atttggattt
    11521 tcttcgcaaa catctattcc accagaacca gctcccgtca ttacacgatg accttcttca
    11581 tcgtaggcag cccaggcaga cgctttagga tcaaaaacaa ataatctttc gcccggtgct
    11641 ttaatttgca ttgggaaata attacgtcct cttttatccc ttaaataatg aacggtccta
    11701 tgtacataac ccgcatcgtc agtaattaat gttgactcat caaactcaac gcacgaagtt
    11761 aatccaaggc acattaaaat aactgcccaa taaatttttt tcatttagat tttatctcca
    11821 caatagtaaa acctggtggc cttaattaat cgcttaattt tcgatactta attcgtgatg
    11881 gtctgttagc ttcatcaccc agtcgacgct ttctatccgt ttcgtattca gtgtaattac
    11941 cttcgaaaaa ggttatttgt gaatcacctt caaaagccat caaatgagtg caaattctat
    12001 ctaaaaacca tctatcgtga gaaatcacta tagcacaacc tggaaaatta aggatggcct
    12061 cttccaaagc tctcaatgtc tcaacatcca aatcattact gggttcatcg agtagcaaaa
    12121 cattcccgcc acttttcaat aattttgcca aatgaacccg gttacgctcc ccgccagaca
    12181 attgagaaat tttcttttgc tgatccgttc ctttaaaatt aaaacgccca acataggctc
    12241 gtgaaggcat ttggaaatta cctatctgca taatgtcatg gccatctgaa atttcttgcc
    12301 acaccgaatt gttggggttt agattgtccc gcatctgatc aacataagcc aacttcactg
    12361 tttctccaat acgaatgacg cccttatctg gttcttcctg cccagttaac atttttaaaa
    12421 aagtagactt acctgcccca ttgggaccaa taattcctaa aataccgcct ttgggaagtt
    12481 tgaaatcaaa attgtcgatt aatatccggt caccgtaaga tttgcatatt ttttcacctt
    12541 ccagaaccaa atcacctaac cgctcaccgg gcgggatata tatttcattg gtttcattac
    12601 gcttttggaa ttctttagaa ttcatttctt caaaacgggc caagcgggct ttatttttag
    12661 catgacgtcc tttaggtgag gttctaaccc attccaactc tgctttaata gcacgttgca
    12721 aagcatcctc ttgctttttc tccatactca accgtgcttc tttttgttca agccaggaac
    12781 tgtagttccc cttatacggg ataccttcac cccgatccaa ttctaaaatc cactccgcag
    12841 cattatccag gaaataccgg tcatgggtaa ttgcaacaac ggtaccagaa aactcttcaa
    12901 gataatgttc taaccaggca acactctctg catccaaatg gttggtaggt tcatcaagaa
    12961 gcaacatatc aggacttgat agcaacaatc gacaaagagc aacacgtctg cgttcaccac
    13021 ccgacagttt tcctattatg gcatcccact cgggtaatct taacgcatca gcagccacat
    13081 cgagctttct ctccagatcc caaccgccac aagcttctat ctcattttgc agctcacctt
    13141 gttccattaa caaggcattc atttcatcat cgctcattgg ttctgcaaaa cgcatactaa
    13201 tcgcatcgaa acgagacagt ttctctttaa tttcagcgac accttcctca actacttcac
    13261 gaacagtttt acttaagtca agctcaggtt cttgagcaag gtatccaatt ttaattcctg
    13321 gctgaggtct tgcttcacct tcatattgtg tatcaacacc agccataatc cgcaatagag
    13381 tagattttcc agaaccattt aatcctaata cgccaatttt tgccccggga aaaaaactta
    13441 atgaaatatc ctttaaaata aacctttggt tctcaacaat cttgctcact ctattcatag
    13501 tgaatatata ctgcgccata atcgctccaa ctctctataa tcagcctttt atcaaacaat
    13561 actaccagtt cagaataact ttccctgact ggcccgatgc cataatttga aatgcgtgct
    13621 gatattcatc tactgggaaa ttatgagtaa taacaggaga aatatttaaa ccgctctgta
    13681 acatagcaat catcttatac caggtttcaa acatctctcg accataaata cccttaataa
    13741 ccagtccttt aaaaataacc tggttccaat ctataggggt ttcttgtggt gggataccca
    13801 ataaagccac atgaccacca tgattcatcg ctttcatcat gtcgtttaat gccatagggt
    13861 tcccggacat ttccagaccc acatcaaaac cctcgagcat tcctagctca ttagccacat
    13921 cacttaattt ttggtatttg acattaaccg ctcggctgac acccatttgc ctggccagtt
    13981 ccaatcgatg atcgttaaca tcggtgataa caacatgtcg tgcgccaata tgtcttacga
    14041 tggctgcggc cattattcct atgggacctg caccagtaat caaaacgtct tcgcctacta
    14101 catcaaatgc caatgcacaa tgagcagcat ttccaaaagg atcgagaata gccgcttgct
    14161 cttctgtaat gttgtcagga agagcaatga cattggtagc aggaagcgcc agatactcag
    14221 caaagcatcc tggtcgattc acccccactc ccagggtatt acgacataaa tgacgcttgc
    14281 cggcgcgaca atttctgcaa aaaccacagg taatatgacc ctcaccagaa actctctgtc
    14341 caactttcaa accttgtact tcttttccaa cctctactat ttcaccataa aattcatgcc
    14401 ctaccgtcat aggtacagga attgtggctt gcgcccattc atcccaggaa taaatatgga
    14461 tgtctgtacc acaaattgca gtccttttaa tttttattaa aacatcgttt actccatatt
    14521 caggaacagg aacatcctgc atccaaattc caggctcttt ttttgcttta accaatgatt
    14581 tcatgtcata cctcaaatta attaggtaat accaccattt cgcaattcat cctaaaaaaa
    14641 tatcaggttg tgaaatgtag agtaatttag tttcagatta cagagaattc cttaccaacc
    14701 gtttcaaacg ccttcagggc tttatccaaa tgatgcaatt caagggctgc agacatttgg
    14761 gtacgaatgc gggccaaacc tttgggtacc actggatagg aaaaccctac aacatagaca
    14821 cccagctcca gcaaccgatt agcgattcgc ccggccaaac tggcatctcc taacatgaca
    14881 ggaataatag gatgttcgcc cgggattaac tcaaaaccta actgagtcat tccttcacgg
    14941 aaatagtgac tattacgctt taatttttct gcaagggagt tatttgtgga caatttatcg
    15001 agcaccacac aagaagtatg cgcaataacg ggggccaatg tattggaaaa cagataaggc
    15061 ctggatcgtt ggcgcaacca atcaatgacg acttgatttg acgcagtata tcctcccgat
    15121 gcgcccccta aggctttacc aagagtacca gttacgatat caattctatc actaactcca
    15181 aaatgctctg gtgttcccct acctgtcttg cccatgaaac caacagcatg agaatcatca
    15241 accatcacca tggcgtcata cttgtcagcc agttcacaaa ttgcaggcaa gttagccaat
    15301 ataccgtcca ttgaaaaaac gccatctgtt gcgatcaaac gaaatctcgc attttttgct
    15361 gcaattaact gttcttccag ggccttcata tcattattgg catatcgata acgtgcagcc
    15421 ttacacagcc tgactccatc aataatactc gcatgattca gggcatcact gataatggca
    15481 tcttcttccg tcaataatgt ttcaaataat cccgtattgg catcaaaaca ggaagaatac
    15541 aatatagtgt cttctttatt taaaaaatgg ctaatttttt gttctagttg cttatgcggg
    15601 gtttgtgttc cacaaataaa acgaacagaa gccatgccat atccgtactt ggctaaagcg
    15661 gcctgtcctt cagctattaa ctcaggatca ttggccaaac ccaagtagtt atttgcacat
    15721 agattaataa cttctctttc gttaactttc acgtcagctt gctgttggct ggaaataaca
    15781 cgctctgatt tgtacaatcc gtctgccttg agtgtttcaa gttcattttg taaataatta
    15841 atgaatcgct caagcactat agtctcctgt atttctggat aaattcataa ataataccaa
    15901 attttaaaca ttctcaccat gagatattat caagaaaatt tcactttcaa acattaatgc
    15961 ttagacataa gctgaattct tgctaaaatt gctccaaagt tttaacctat ccaatgatta
    16021 ttaagacggt agagcaaatg aatcacagcg cacgcataaa tatgatcaaa caacagttac
    16081 gaactggtga tgtattaaac gaatccatcc ttgacttata cgatgagatc ttaagacatg
    16141 agtttgttcc tgaacctttt tctcattttg cttattcaga catgcaaatt cctttggcac
    16201 acggtcaaag gatgttaact ccattggaag aaggtacaat tttacaatcc cttgatctta
    16261 aaggccatga aaccgtgttg gaagtgggaa caggaaccgg ttttatgacc gctttactca
    16321 gtaagttatg taaaaaagtg atcagtattg actattattc tgaatttact accaatgcaa
    16381 aacgcaaact tgaagagcat aattgtaata atgttgaatt aattaccgga gatgcttgtc
    16441 gtggatggtt ggaaagtgca ccatacgatg ttatcgtatt cactggtgcc atggaaaagc
    16501 tgacagatac ccataaacta caaattttac ctggtggcaa gttattcgca atccttggta
    16561 aatcccctgt aatgaaagct tatctttttc agttggatca taatgcaatc tggacagaat
    16621 ccatgctttt tgaaacggat atacctcctc tggtagatca attgaaaccg aaagaatttg
    16681 ttttctaggg aaagctcgaa tgagaaaatc tctgttttgt tggattttaa ctttaggtgt
    16741 ttcaacccat gcgtttgcaa cagacctaat ggatatctat caacaagctc tggagaacga
    16801 taccattttt aaagaggcat atgataccta tatgtccagt actgaagcca ttcctcaagc
    16861 tcgagcagca ctttaccctc aagtaggcct tggttcacag gctggccgca attatcagga
    16921 tgctgttgct ggagcgttca gtgcgaatca gtactatgga agctatttgt ggcaagtcaa
    16981 ggcttctcag gcactcttta actatcaggc atgggcccag gtagcccagg ctaaagcgtc
    17041 tgttaaagca gcacaagcca gtttcaatga tgcagctcag aatttaattc taagaacagc
    17101 caaagcctat tttgatgtcc tgtttgccaa agacacctta gactttgccg aagcaaaaaa
    17161 acgggcgaac aaaagacaat atgatcaagc aactcaacgt tttcaagttg gtctggatgc
    17221 cattacttct gtatatgaag cgaaggcagc gtatgatcaa tccatagcga cagttatcgc
    17281 cgcacgcaat aatcagatta accagagtga aaatttacga aaacttacta atcacgtata
    17341 cgaaacactc gcgccactca aagacagtaa aattcctctt gttaaaccag agccgaatga
    17401 cgtgaatcaa tggatagata ccgggcttaa gcaaaactat aaattatatg ctgcaaagta
    17461 taatcttgag gtggcaaaag agaatgtaaa agcgatctct gccggtaact ggccagtatt
    17521 ttcgctacag agcaatgcca gtcaggtaca taataacgca tctggcaata atgtttttat
    17581 tccatcaaag caaacccagg ctaatattgc aatcgcaatg aattttcctg tttttcaagg
    17641 cggcctggtc caggctcaga cccgacaagc tcaatataac tttcagtcta cgagtgaaaa
    17701 actggaacaa acgtatcgtg atgtcattgt aaacagtcgg attgctttca ataccatcac
    17761 tgatgggata agtaaggtaa aggcagacag acaaacagtc atctccgtgc aaaattcttt
    17821 gcaaagtact gaagctcaat ttgaagttgg tactcgaacc atggtagatg tggtcaatgc
    17881 tcaacaaaga ttgtttgaag cacaggaaca attagcccgg gatcaatacg atttgattaa
    17941 ttccatcctt acgctgaaat accttgcagg gacattaaac gttaatgatc tcgaacagat
    18001 taactcatgg ttagcaacta cacgtgtcaa tggattttca cccgtagata acaaaacctc
    18061 caaataacaa atttaggttt tcttatgtct tccaatcctt cttcagttga aaaaaaatta
    18121 ctccattata caggtaaagc catagctgat tttaatatga tccggcgtgg tgacagagtc
    18181 atggtatgtc tgtctggcgg aaaagattca ttcacactat taacaattct taaccagctc
    18241 aggatcaaat caggaaataa atttgaaatt tttgcgttta ctctggatca agcccagcca
    18301 gggtggaatg atgcctgttt gcgtcaatgg ttggctgaaa aatccattcc tcatgaaata
    18361 ctaacccgag acacatacag tatagtgaag gaaaaaattc ctgaaggcaa aacctactgt
    18421 tctttgtgtt ctcgcttgag acgtggaatc atctacagat atgctgaaga aaacggattt
    18481 aataaaatag cattaggtca ccatcgtgat gatcttattc gtacattgat gatgtcaatt
    18541 ctatacaatg gcgatatacg ttccatgcca cccaaactac tgagtgataa taaaaagcac
    18601 atagtcatcc ggccactgtg ctatgtacag gaaaaagata ttattacgtt tgcatcagaa
    18661 caagcctttc caattatccc atgcaatctt tgtggctctc aagaaaatct aatgcgcaaa
    18721 aaagttgcct cactcattga tcagcttgcg attgaaaacc ccaaagtccc cagtaacatg
    18781 cttcatgcgc tgcaaagttt gaagccaagt caattgatgg atcaaaattt ttggaatttt
    18841 aaaaatctgg aagatgggct tgaaaccgcg cagtcagttc aatgtgaaga agtatttaat
    18901 gcacaggaat ttgagtttga ggatgaaaaa atttgactta tgaaaattat tatctaatat
    18961 ttcttttcat ttttgcaata taatgaaata attgacacgt aaaataagct tattattttg
    19021 acaaatggaa ttaaatatat aaaaataggc tttcaaatta tcacattaac tgtgactttg
    19081 tttcattaac ccgatactga attcaa
//
LOCUS       DATNSN010000050        18546 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.050, whole genome shotgun sequence.
ACCESSION   DATNSN010000050 DATNSN010000000
VERSION     DATNSN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.050"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1300)
                     /locus_tag="VAH76_001436"
     CDS             complement(<1..1300)
                     /locus_tag="VAH76_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO1386068.1"
                     /translation="MNCNLLIIKIFFSFILIFIGISNPAISGRAPDVWDVLRSEFTLS
                     HEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESA
                     YDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
                     GNWILAIAAYDSGEGTISRALKSTARNGRKVQFWNLPVPRETKVYIPRLLALAEIINN
                     PKRYKIALPNIPYLPYFEEVNIGSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYK
                     PFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLN
                     QLTSNKVQLNQSLLIPSSKNSPVVAKIPPQEKKPATKQLAPMKNVRIIHIVQNNDNYQ
                     KIQKAYGVAAKEIQNWNKLANNAPLRKGQRLVIWKRVKQPV"
     gene            complement(1414..2178)
                     /gene="gloB"
                     /locus_tag="VAH76_001437"
     CDS             complement(1414..2178)
                     /gene="gloB"
                     /locus_tag="VAH76_001437"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEO1386069.1"
                     /translation="MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAGPIIRFAQSNQ
                     LTLRTILLTHHHYDHIGGVDLLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLH
                     FHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
                     NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFL
                     RTDKEQVKQYALSHGANSSDSLDVFKVLRNQKDSFK"
     gene            complement(2182..3030)
                     /locus_tag="VAH76_001438"
     CDS             complement(2182..3030)
                     /locus_tag="VAH76_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEO1386070.1"
                     /translation="MLSYQHGYHAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSG
                     RGIYDLKDKQSLKTEEYKEGINPVWLDRENLPSLFLEYISVIKQINLNSTLRYYPGSP
                     YFAINQLRSQDRLYLCELHPTEYNFLLKLPHFNKKVYVNHTDGVSKLNALLPPPEKRG
                     LIFIDPSYERKEEYKEIPYAIKNAYSKFSTGLYCVWYPVVNKAWTEQFLRKMREISSK
                     SVRIELHLNPLINEGMTSCGLWIINPPYTFPSEIKIVLETLTTYFNPGSSSYIIESGS
                     KLCHEL"
     gene            3318..3527
                     /locus_tag="VAH76_001439"
     CDS             3318..3527
                     /locus_tag="VAH76_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386071.1"
                     /translation="MGLHEEFEDQSFSDYNYDEEIDDHIDDLNQKKKIRRMLEDRLER
                     KRLKEEFKDDFDELSGEFDWDELDK"
     gene            complement(3530..4384)
                     /gene="folD"
                     /locus_tag="VAH76_001440"
     CDS             complement(3530..4384)
                     /gene="folD"
                     /locus_tag="VAH76_001440"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEO1386072.1"
                     /translation="MPASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGND
                     PASVIYVSNKRKACEEVGITSHSFDLPAETTQEKLIELINELNQSDKIDGILIQLPLP
                     KHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
                     HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIAT
                     DWLREHQVVIDVGMHRLPDGSIRGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTM
                     MSAARLRE"
     gene            complement(4471..4671)
                     /locus_tag="VAH76_001441"
     CDS             complement(4471..4671)
                     /locus_tag="VAH76_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386073.1"
                     /translation="MNRENEVIEIFLMDISKKEKCKLLQDFLLDCKNEMEAQDQNMHP
                     EVHHNLSQAYQLAQNYLRKLQE"
     gene            4940..7525
                     /locus_tag="VAH76_001442"
     CDS             4940..7525
                     /locus_tag="VAH76_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein FimV"
                     /protein_id="HEO1386074.1"
                     /translation="MKKTVLHAAIFSLTMPISLYALGLGEMKVQSSLDQPFSAEIELI
                     DVGADPLASIKVSLADPEQFEQIGLERTAVLSLLRFQVERNPKGAPVVKVSSTERMTE
                     PYMEIVVDLTWPKGQLYKAYTVLLDPPGYQLGITTAQSRPVYYKKKSASYSNEPGVIE
                     KTVITTVEHGPVSKNDSKKKTTYGPTVTNENVWQIAQRYKTSKLILPQVVLAIVGANP
                     EAFKDGNLNGLKVGVRLVIPSTEKIVKVPSDLAIEEVMAHDKAWNEHTSINHALAPPY
                     INSSIVDVTPKQAKASSIPVIPKFTIGENSNIPTPQQLIPDSTSVSIDASTQQQPVQN
                     NNIVQTTEQNEKIKAEISITTAAVESVREANALLMEQLRILQEQNKKLQEKLDKREKE
                     IELMRTQVQTMLKERKAIASQVSSLPNNEQGMNLWPYIILLIFAAGGAAFAYWYLRQR
                     QQREDISPYKTDSDSSPKPFMPLVEPAKYEPDTQADDKETSVQEKISDSEKEIGVGVS
                     KADWTEYQPKEMDKIEKIDAVESETVEDVTYLGDTEQLRAKEYTELEPEVQTKPEAQT
                     KPEAQTKPEIQTKPEIQTKPEVQAKPEVQAKPEVQAKPEVQAKPEVQAEPEVQAGENQ
                     IMFEPVMELEQLDVNRDDEVDNSQLSEDETQQTEGHQSSEMIEFESGLHELIKDKPKT
                     KITESTDEGDNNGIDFIPLTINDADKTLKYEQEIEKSDKKEDKKSEEFSTKENKFDLD
                     NSDFDMKNLESEPEINQEEKEDHISEITEFDQKSTQEEKTEKGISISQSSDEATFTKS
                     SNESNQTSNPLKSKKALDILLDLAKTYISMGDIETARSSLEEVIEHGSVSQKEQARQL
                     LDQLK"
     gene            complement(7581..8048)
                     /locus_tag="VAH76_001443"
     CDS             complement(7581..8048)
                     /locus_tag="VAH76_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEO1386075.1"
                     /translation="MLYFYLKFIHVLSSTILFGTGIGTASVMIYGHRTKNPIVIAAIS
                     KYVVFADWIFTGTSGILQPLTGFAMVYLAGFSWTSFWILGSILGYVVAACCWFPVVYL
                     QIKMRDLAIETVKNNTTLPKKYDRYFLYWFILGWPAFLSLIGVFYLMTNKPVF"
     gene            complement(8048..9346)
                     /locus_tag="VAH76_001444"
     CDS             complement(8048..9346)
                     /locus_tag="VAH76_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO1386076.1"
                     /translation="MKILVTGASGFIASQFVTDLLIAGHEIICCVRNTKHTQRIFPGA
                     QVIFCDFINDTKPEIWSKRLQGIDVVINCVGILYHPDERIIWNVHYETPKALFDACIN
                     SGVKKIIQISALGIDKVDVSYAASKKAIDDYLLTLSIPSVIVRPSYVYGKGSYGGSSL
                     FRGIAGTPFFTAIPGQGTQKFQPISLNDLSQAIVRLVSTPVTETIILHAVSKKIITLE
                     EIIIKLRSWLGLTKTRIIHIPLTFIRLLTRLGDLLPYSTINTVSYKMLASDNITTQEE
                     TQRFADYVNFTPRDYEEGLYSQPSSVQDHWHAQLYFLKLPLRLGISFVWIWTALCCLF
                     FYPKSDSLSLFAQIGFSSLGQLFLFYASALWDGLLGITMLFNYRFKLTSILQLITILV
                     YTVIITIKLPYLWLDPFAPIAKNIPLLIAILIGLGLESDK"
     gene            9590..10378
                     /gene="truA"
                     /locus_tag="VAH76_001445"
     CDS             9590..10378
                     /gene="truA"
                     /locus_tag="VAH76_001445"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO1386077.1"
                     /translation="MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISV
                     VCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARY
                     SAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
                     QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDV
                     LNAKDRKLGAETAPSYGLYLVQVTYPKEFGLLQNNPGPLFLWEK"
     gene            10379..11002
                     /locus_tag="VAH76_001446"
     CDS             10379..11002
                     /locus_tag="VAH76_001446"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEO1386078.1"
                     /translation="MNPSRIRIKMCGMTRSEDIQYAIDLGVDAIGLIFYPKSARNVSL
                     EKARIIVNNIPPFVDIVAVLVNPEQSFVQLIINEIPVQLLQFHGEESSEFCRQFNKPF
                     IKAIHPKTAIQIQSAVDEFFDASAILLDTPSDKERGGTGLTFDWNIIPENLSKPYILA
                     GGLNESNILEAITMCHPYAVDVCSGIEASPGVKDHLKMSRFIKAIWG"
     gene            11004..12203
                     /gene="trpB"
                     /locus_tag="VAH76_001447"
     CDS             11004..12203
                     /gene="trpB"
                     /locus_tag="VAH76_001447"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEO1386079.1"
                     /translation="MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQD
                     FLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
                     VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEAR
                     AQHMEKTGRLPDALVACVGGGSNAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASL
                     IAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAIND
                     SEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVA
                     AIDGITI"
     gene            12229..13047
                     /locus_tag="VAH76_001448"
     CDS             12229..13047
                     /locus_tag="VAH76_001448"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEO1386080.1"
                     /translation="MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSG
                     ADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGY
                     LNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
                     ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPE
                     MAAQVAEFADGVIVGAALINEIIEAYEAKKDPLQASGALLSSMRQAIDNIGSMV"
     gene            13044..14699
                     /locus_tag="VAH76_001449"
     CDS             13044..14699
                     /locus_tag="VAH76_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEO1386081.1"
                     /translation="MTEQTEKRAHFIRQLIIDDLANGKHQGIVTRFPPEPNGYLHVGH
                     AKSICLNFGLAEEFKGKCFLRFDDTNPIKEEEEYVNAIIDDVRWLGFEWCAMTHSSDY
                     YHELYELAIYLIKKDMAYVDSLSMDEIRAFRGTLQEPGRESPYRNRTIEENLDLFARM
                     KAGEFPDGTHVLRAKIDMKSGNLNMRDPVLYRIRHARHQRTGDEWCIYPMYDYAHPIS
                     DALEKITHSLCTLEFQDHRPLYDWLVDNLPLPAKPVQTEFARLNLSHTVTSKRKLREL
                     VEKKIVTGWDDPRLPTLRGMRKRGYPPAAIRQFCEIIGISRSDSVIDMSLLEECVRAE
                     FNKTAKRALCVMDPLKVVIVNYPENKVEQLQAAFYPQNPESESRNLPFSREIYIEKSD
                     FMENPPNKYFRLSPGAEVRLRHAYVIKCQQVIRNDQGEITELHCTYDENTLGKNPEDR
                     KVKGVIHWVSSSHAYPVTIYQYDRLFTDPNPAREDDYFQFLNHNSLQTLQGFCEPAMA
                     EQAEGDVFQFERLGYYCVNSVAEGCVQAFHRVVDLKDTWGKVS"
     gene            14709..16079
                     /locus_tag="VAH76_001450"
     CDS             14709..16079
                     /locus_tag="VAH76_001450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO1386082.1"
                     /translation="MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSM
                     VAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHA
                     LNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSENGDVCYEVDTFPEYGKLSHKDI
                     EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWSSPWGEGRPGWHIECSAMAMHELG
                     EQFDIHGGGLDLQFPHHENEIAQSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYT
                     IADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLYQAVKDVPPQTADSK
                     LDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALVHTLKILAGILGFLQKD
                     PESFLQSGLAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTT
                     WRRIAE"
     gene            16251..18164
                     /locus_tag="VAH76_001451"
     CDS             16251..18164
                     /locus_tag="VAH76_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HEO1386083.1"
                     /translation="MNIKTYLKKNDLTLRFVGWFFLINSFIFWLIGLGYLKSILLNAS
                     LFKNYIADYSSLSGKILILVFSVLNYFTYMMFLAFVPAIPIIIMAFFLPYKRIIWIIS
                     VVAATTSLIFLFVDSQVFLMFKFHLNKTILSFVFNSDWSVIFTFSRYELILSFFMIVF
                     ILFLESIVAWLVWKKIVLKERLKIGKTISLFWLGGALISYFILLLSIAQNNNLFAQQI
                     PNLPLYNQLIAYTIPDKNADDILRRYSEHHFTQPLFPNYPLHYPLHPMQCKMPEKPYN
                     IILIMVDSLRYDSLRAKYMPNTAQFAKRSWQFSQHMSAGNATQPGLFSLFYSIPSNYS
                     TAVLEQKKSPVLIDLLLQHGYQTKIIWSGSMAPLPLDQTIYKKIANLNINGAPIDDTG
                     DKDRYSTSEAIQFLTDPKTNNPFFLHIFYNAPHDYCRYQSFPQLYKPAIEECVRIGMT
                     NHDDPVPYYNRYLNAVTFIDQEISKVLGVIEKKGYLKNSIIIITSDHGQEFNDNRQNY
                     WGHTSNFTPIQLHVPLIIHWPGELARRFNYLTSSYDIVPTILQRLFACKNPVSDYSIG
                     YNLLIEGNRASFLLVGSYINMGIFEADRATALETSGRITITGMKAEPLDDAVPRTDVI
                     TQALDLMRKYYGK"
     gene            18292..>18546
                     /locus_tag="VAH76_001452"
     CDS             18292..>18546
                     /locus_tag="VAH76_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386084.1"
                     /translation="MANQTRENLINKAKNGSESIALIYSPIELHEYIVEIISKDSDHF
                     IHKGKEICHFNSIDEAVSRATELGAKEFFLCADNTYDECGS"
BASE COUNT         5878 a         3235 c         3733 g         5700 t
ORIGIN      
        1 caacaggttg tttgactctt ttccatatga ccagccgctg gcctttacgt aatggcgcgt
       61 tatttgccaa tttattccag ttttgtatct ctttagccgc aaccccataa gctttttgaa
      121 ttttctgata gttatcatta ttttgtacga tatggataat acggacattt ttcataggtg
      181 ccagctgttt agtagcaggt tttttttcct gtggaggtat tttagctact actggggaat
      241 ttttgctgct cggaatgagt agggattgat taagttgaac tttattgctg gttaattgat
      301 tgagttgttt tatcaaattg actgttgtgt gatatctctt tgcaatagca tccaagctgt
      361 cacctctttt tacttgatgt ttagtccagt ttacgcgctt atcttcgggt atattcgata
      421 gattcaagtt aaatctttcg actttttcag caggtattag taatttaaat ggcttataag
      481 gtgcagttgt ccatcgatta taaccgggat ttagtttaat caactcctta taactgattc
      541 cggcgagttt agcggcatga tttaaatcaa tttgactacc aatattgact tcttcaaaat
      601 aaggaagata gggaatattg ggaagtgcta ttttataccg ttttggattg ttaataattt
      661 ctgccagagc taataaacgc gggatatata ccttggtttc tctgggaact ggcagattcc
      721 agaattgcac cttacgtccg ttacgtgcgg ttgatttgag cgcccgcgaa attgttcctt
      781 ctccagagtc ataagcagca atagctaaaa tccagttacc actaaaatat ttatttagat
      841 agacgaggta atttaatgcg gcatcagttg aagggccaat gctgcgtctt ccatcaaacc
      901 accagtcttg ctttaatcca agatcgcttc cagttcgagg catgagttgc catataccag
      961 cagcgcctgc tcctgaatag gcaaaaggat catatgcact ttctatcatg gggagtagcg
     1021 ccaattctcc gggcagtttt cttttcttta tctcacctaa cacgtggtaa atgtaaggtt
     1081 cagattggct acttactttg ttgatataac taggatgaga aattagccat ctgatttgtt
     1141 cttgcacttc tgggcgagtt acttcatggc ttaaggtaaa ttcactacgt aatacatccc
     1201 atacatctgg ggctctgcca gaaattgccg ggttagaaat accaataaaa ataaggatga
     1261 atgaaaaaaa tatttttata atcaataaat tacaattcaa tggtcttacc tgttattata
     1321 attggctaat cagtttacta aaaattaatg aaggattaaa ccctttttgg tttaatttgg
     1381 cgcaaatggg tttttatttg atcctttgtt ttattatttg aacgagtctt tttgatttct
     1441 caatacctta aaaacgtcca gagaatctga agaattcgca ccatgcgaga gagcatattg
     1501 ttttacttgc tctttatcag tgcgtaaaaa aggattaatt gataattcca agtctatatt
     1561 agacgggaga gtacatggtg atggttgttt cagaatttgt tgcatgtaat tgatgactga
     1621 tgaattgcag ggctcaacgg tatgtgcaaa ttttaaattc tgtaaggtgt attcatgagc
     1681 acaaaaaact ttagtatttc gcgggagttt tttaaataag agcagggatt catgcaattc
     1741 ctctatagtt ccatcaaaca ctctaccgca accagcagaa aatagagtat ctccacaaaa
     1801 taaccatcct tgttgtggtt cataataact gatatgggta gaagtatgcc caggattaaa
     1861 taaaatatga aagtgaagag agcccacttg aacagattga ccttgtttaa ttgggtgggt
     1921 tacattgttg attcgttcat ctatggggcc atagacctta caagatggcc attgcttgat
     1981 tagcaagtca acacctccta tatgatcata gtggtggtgg gttaataata tcgttcttag
     2041 tgtcagctgg ttggattggg caaagcgtat tattggtcct gcttctcccg gatcgacaca
     2101 atctaatacg ccagctattt tatcaataaa ggtccagata taattatctg aaaaagccga
     2161 aataggcagg atagtcataa tttataactc gtgacataat tttgaacctg attcaatgat
     2221 ataagaagat gagccagggt taaaataagt tgttaatgtc tctaaaacta tcttgatttc
     2281 tgaagggaaa gtatagggag ggttaattat ccaaagacca caactagtca ttccttcatt
     2341 aatgagtgga ttcaagtgaa gctcaatcct taccgatttg gaactaattt ctctcatttt
     2401 cctcaagaat tgttcagtcc atgctttatt gacaacagga taccaaacac aatataagcc
     2461 ggtagaaaat tttgagtaag cattttttat agcgtatgga atttctttgt attcttcttt
     2521 tctttcatat gagggatcaa taaaaattaa cccgcgtttt tctggtggcg gcaaaagggc
     2581 atttaatttt gaaactccat ccgtgtggtt aacataaact tttttattaa aatgaggaag
     2641 ttttaataag aaattatatt ctgttgggtg taattcacat aagtataatc ggtcttgaga
     2701 gcgcagttga ttgattgcga aataggggga gccaggataa taacgcaatg ttgaattaag
     2761 atttatttgc ttaattacac taatatattc aagaaaaaga gaggggagat tctctctatc
     2821 gagccatact ggattaattc cttccttata ttcttctgtt tttaaagact gcttgtcttt
     2881 taaatcatat atccctcgac ctgaatgggt ttcaagatag aataagggtt tatctttgtg
     2941 ggttagataa gccaatagtc gagttagggt gatgtgcttg atgacatcag caaaatttcc
     3001 ggcatgatag ccatgttgat aactgagcat gaaagagggc ttcaaaagat attttccaaa
     3061 ttatatcaaa ataatggttt atgctatagc tgcatattag acttcttgca taagtggtct
     3121 cttttatttt acttccgcat cgtaagcatt tcttcaacac ttatgtactg catgtaaact
     3181 ccacctctca aaacgtatgc gacatgtcct gaagcaaaat aatcaggtta tgcaagaggc
     3241 ctattttgct tccagttact ttatttcata atttatgcac tatacttttt ttgaggggag
     3301 taaattggga gactaagatg ggtttacatg aagagtttga ggatcaatca ttttcagatt
     3361 acaactatga cgaagaaatt gatgatcata tagatgattt aaatcaaaaa aagaaaatca
     3421 ggcgtatgct tgaggatagg cttgaacgta agcgtctaaa agaagagttc aaagatgatt
     3481 ttgatgagct aagtggtgaa tttgattggg atgaactgga taaataactt cattctctta
     3541 atcttgctgc tgacatcata gtattttcaa gcaaagtgac aatagtcatt gggccaactc
     3601 cccctggaac aggtgtgatc caggcaactt tttcgactgc cttcttaaaa tcaatatcac
     3661 ctctgatact gccatcgggc aaacgatgca ttcctacatc aatcactact tgatgctctc
     3721 tgagccagtc agtggcaatg acgtccatct ttccagtggc tactattaga aaatcagcaa
     3781 tctcaacaaa cttctgtaat tgttgggtaa acttgtggca tattgttacg gtagcaccag
     3841 ctaatagtaa ctccagactc attggccttc caacaatatt ggatgcccca atcactacgg
     3901 catgttttct ttttacattg agctcatagt gatgtaataa attcataatc cccaagggag
     3961 tgcatggtct taaaaatggg tttcgttgag caagtcggcc taaattataa ggatgaaatc
     4021 catccacgtc tttctcgggt tttatgtgtt ctattatagt tctttcgtta atatgtttgg
     4081 gcaaaggtaa ttggattaaa ataccatcaa ttttatccga ttgatttaat tcattgatca
     4141 gttcaattag tttttcctga gttgtttcgg caggcaaatc gaaagaatga gaggtgatcc
     4201 ctacctcttc acaagctttt cttttgttac tgacataaat cactgatgca ggatcattgc
     4261 caattaaaac aacagcaagt cctggcgccc tttgcccttg ctccacatgg tattgtactc
     4321 tttgctttag ttcagttctg cgaagggcag agatttctct tccatcgatt agtgatgccg
     4381 gcattttctt ctcgcaattt ttatggagta cattatgaat tacgggttat tctaatgcaa
     4441 tatgattcat atgcaacaag gaaaatacac ttattcttgc agttttctta aataattttg
     4501 cgccagctga taagcttgcg ataaattgtg atgcacttca ggatgcatgt tctgatcctg
     4561 agcttccatt tcatttttgc aatccagaag aaaatcttgt aacaatttgc atttttcctt
     4621 tttgctgata tccattagaa atatttcgat tacttcattt tctctattca tcgctattcc
     4681 aaatttaatt ttactaatcc ttaatataat tatagttgaa ggtctattgg agttatgaat
     4741 tttcctggtt ggattttaaa aggccacaat tgatttggct tgttaaatta gatgaaatat
     4801 gtggtgtaat tttatacatc atcttatgat ttttagggaa taatttcgtg ttttcttgaa
     4861 tagactaacg gataatgata ctttttactt aggcaattaa atcatcaaca tcttgtatca
     4921 caggtgcatg gagctctgat tgaaaaaaac tgttctgcat gcggctatat tctcattgac
     4981 tatgccaata tctctttatg cgcttggctt aggggaaatg aaggtacagt cctcattgga
     5041 tcagcctttt tcagccgaaa tcgaattaat agatgtagga gcagatcctc ttgccagtat
     5101 caaggtgagt ttggctgatc cggagcaatt tgaacaaatt ggacttgagc gaactgcagt
     5161 cttgtctttg ttacggtttc aggttgagag aaacccaaaa ggtgcccctg ttgtaaaagt
     5221 atcgtctaca gaaagaatga cagaaccata tatggaaatt gtcgttgatc taacctggcc
     5281 aaaaggtcaa ttatacaagg cctatacagt attattagac ccgccaggat atcaattggg
     5341 aattaccact gctcaaagca ggccagttta ttacaagaag aaatccgcaa gttacagtaa
     5401 tgagcctggt gttattgaaa agactgtgat tactacggta gagcatggcc ctgtatcaaa
     5461 aaatgacagc aaaaagaaaa cgacttatgg cccaactgta accaatgaaa acgtttggca
     5521 aattgctcaa agatataaaa cttcaaaatt gattttgcct caggttgtat tagcaatagt
     5581 tggtgcgaat cctgaagcat ttaaggatgg aaatttaaac ggtttaaaag ttggcgttcg
     5641 gctagttatt ccatcaactg aaaaaattgt aaaagtgcct tctgatttgg ctattgaaga
     5701 ggtgatggct cacgacaaag cctggaatga acatacatct attaatcatg cattagctcc
     5761 gccatacatc aacagttcaa tagtggacgt gactccaaaa caggctaaag cttcttcaat
     5821 cccggtaatt ccaaagttta ctattggaga aaactccaac attccgacac ctcaacaatt
     5881 aatccccgat agcacttcag tttcgataga cgcatctacc caacagcaac ctgtacaaaa
     5941 caacaatata gtacagacaa cagaacaaaa tgaaaaaatc aaggcagaaa tttcaattac
     6001 aacagcagca gttgaatcag tccgggaagc aaatgcatta ttgatggaac aattacgaat
     6061 tctgcaagag caaaataaaa aattgcagga aaagctggat aagcgtgaga aagaaataga
     6121 gttaatgcgt acgcaggttc aaaccatgtt gaaggagcgt aaagccatcg cgtcccaagt
     6181 cagttcactt cccaataatg aacaagggat gaacctttgg ccgtatatca ttttattgat
     6241 atttgctgca ggaggagcag cgtttgctta ttggtacctc agacaacgcc aacagagaga
     6301 ggatatttct ccttacaaaa cagattctga ttcaagtccg aagccattta tgcctttagt
     6361 tgagccagcg aaatacgaac cagataccca ggctgatgat aaggaaactt ccgtccagga
     6421 aaaaatatct gacagtgaaa aagaaattgg agttggtgtt tctaaagcag attggactga
     6481 gtatcaacct aaagaaatgg ataaaatcga aaaaattgat gcagtagaat ctgaaacagt
     6541 tgaggatgtg acatatcttg gagatacaga acagttaaga gcaaaagaat atacagaatt
     6601 agaaccagaa gttcagacta aaccagaggc tcagactaaa ccagaggccc agactaaacc
     6661 agagattcag actaaaccag agattcagac taaaccagaa gttcaggcta aaccagaggt
     6721 tcaggctaaa ccagaggttc aggctaaacc agaggttcag gctaaaccag aggttcaggc
     6781 tgagccagag gttcaggctg gagaaaatca aattatgttt gagcccgtta tggagcttga
     6841 gcaattggat gttaacaggg atgatgaggt tgataattct cagttaagtg aagatgaaac
     6901 tcagcaaact gaaggccatc aaagttctga gatgatagag tttgagtcgg ggttacatga
     6961 attaatcaaa gataagccca aaactaaaat aacggaatca acagatgaag gagataataa
     7021 tggaatagat ttcattcctt taactataaa tgatgctgac aaaacattaa agtatgaaca
     7081 agaaattgag aagtcagaca aaaaggaaga taaaaaatca gaagaatttt ctacaaagga
     7141 aaataagttt gatttagata attcagactt tgatatgaaa aacctcgaat ccgaacctga
     7201 aataaaccag gaggaaaaag aagaccacat ttcagaaata actgaatttg accagaaatc
     7261 tactcaggaa gaaaaaacag aaaaaggtat ttctatatct caatcttctg acgaggcgac
     7321 attcaccaag tcatccaatg aaagtaatca aacctccaat ccattgaaga gtaagaaggc
     7381 attagatatt ttgttagatt tggcaaaaac ctatattagt atgggcgata tagaaacagc
     7441 aagaagttct cttgaggaag tgatcgagca tggaagcgtt agccaaaaag aacaggccag
     7501 gcagttgctg gatcaattaa agtaataaat aacacagaaa ttacgaaacc ccctaatctc
     7561 ttaatattca cctttgatgt ttaaaaaaca ggcttgttag tcatcaaata aaatactcca
     7621 ataagactta agaatgcagg ccaacccaga ataaaccaat aaaggaaata tcggtcatat
     7681 ttttttggta aggtcgtgtt attttttacg gtctctatgg ctaaatccct cattttgatt
     7741 tgcaggtaga cgacaggaaa ccaacaacat gccgcaacga catacccaag aattgaaccc
     7801 agtatccaga aagaagtcca agaaaatccc gcaaggtaaa ccatggcaaa ccctgtgagt
     7861 ggttgtaata ttccagatgt accagtgaat atccaatcag caaaaactac atattttgaa
     7921 atggcagcaa taacaatcgg attttttgtt ctatggccat aaatcatcac acttgctgtt
     7981 cctatgcctg taccaaaaag tatcgttgaa ctcagcacgt ggataaactt taaatagaaa
     8041 tagagcatta tttatcagac tccaaaccca gacctattaa aatagctatt aacaaaggaa
     8101 tgttcttggc tatgggagca aatggatcta accaaaggta aggcagtttg atggtaataa
     8161 ttaccgtata taccaaaatc gtaatcagtt gcaatatact ggtcaattta aatcgataat
     8221 taaacaacat ggttatacct aaaaggccat cccataaagc tgaagcataa aataagaata
     8281 actgccctaa ggatgaaaaa cctatttgag caaacaaaga taatgagtca cttttagggt
     8341 aaaaaaacaa acaacataag gccgtccaaa tccagacaaa ggatattccg agtcttaggg
     8401 gaagtttaag aaagtataat tgcgcatgcc aatggtcttg gactgaactc ggttgactat
     8461 agagaccttc ttcatagtct cttggagtaa agttaacata atcagcaaat cgctgtgttt
     8521 cttcctgagt agtaatgtta tcactggcaa gcattttata cgaaacagta tttatagttg
     8581 aataggggag caaatcacca agtcgggtca atagtcgaat aaatgtcaat ggaatatgta
     8641 ttattctggt tttggtcaac ccaagccagg aacgcagttt tattattatt tcttctagcg
     8701 tgattatttt tttgcttacg gcatgcaaaa taatagtttc ggttacaggc gtactcacca
     8761 atcgtactat tgcttgagat aggtcattta gagagatagg ttgaaatttt tgtgtccctt
     8821 gcccaggtat agcggtaaaa aaaggtgttc cagcaatccc tctgaataga gaagaccctc
     8881 cgtaagagcc ttttccatac acataagagg gtcttactat aactgacggg atagagagtg
     8941 tcagtaagta atcatctatc gcttttttac tcgcagcata actcacatca actttatcaa
     9001 ttcccaaagc agatatttga ataatttttt ttaccccaga gtttatacac gcatcaaaaa
     9061 gtgcttttgg agtttcataa tggacattcc atatgattct ctcatcaggg tggtataaaa
     9121 tccctacaca attaataacc acatcaatcc cctgcaatct ttttgaccag atttcaggtt
     9181 ttgtatcatt gataaaatca caaaagataa cttgtgcccc aggaaaaatc ctttgcgtgt
     9241 gtttggtatt ccgtacacaa caaatgattt catgtccagc aatcagcaga tcggtaacaa
     9301 actgtgaagc gataaaccca gatgcgcctg ttaccagaat tttcataagg atctcttatt
     9361 tttgattaca attataatag aaatactata acgaatttaa ctcctactat tcaaatttcc
     9421 tcaccaggat taattttaat caacaccagt ttggcatatt taggttaaat gaaatagtat
     9481 gagttcatgc aaaatgataa aacccataca ggcaaagagt taatactcta aaaattatag
     9541 acaaaacaaa gagggtgtta taaattgacc cttttatcag gaaaattgaa tgcgtattgc
     9601 cttagtggtt gaatacgatg gtagccaata tcacggctgg caggctcaaa cgggtttgca
     9661 tactattcaa caagctgttg aatttgcatt atcaaaagtt gcagacagtt ctatttctgt
     9721 tgtctgcgca ggtagaacag atactggggt ccatgcgacc aatcaggtga ttcattttga
     9781 ttgtgagaaa gatcgcagca tacgagcttg gatacatggt gctaatacct ttttacctaa
     9841 agacatctgc gtcaaatggg gcaaggaaat gccagaaaat tttcatgctc gttattctgc
     9901 tgtgagtcga cggtatcgct atgtcattta taatggagcc atacggcctg ggttattacg
     9961 cggtaatgtg acatggcaat atcggcagtt ggatcatcgt ttgatgcaac agggtggaca
    10021 atgtcttctg ggggaaaatg attttacttc attccgctcc gtcgaatgtc aatctaatac
    10081 accaatgcgt aatattcatc aattgcaagt tacaagacat ggtgacttgg ttgttcttga
    10141 tattacagcg aatgcctttt tacatcatat ggtaagaaat atagctggtg tattgattgc
    10201 tgttggctcg ggaaaacacc ctgttggttg ggttaaagat gttttaaatg caaaggatag
    10261 aaaattaggc gctgaaactg caccgtcata tggcctatat ttggtccagg tgacttatcc
    10321 caaagaattt ggattgttgc aaaataatcc tggcccttta tttctttggg agaaataatt
    10381 gaatccatct cgtataagaa tcaaaatgtg cggaatgaca cgttcagaag atattcaata
    10441 cgccattgat ttaggggtag acgccattgg cttaatattt tatcccaaaa gtgcgcgtaa
    10501 tgtctcactg gaaaaagcca gaataatagt caataatata cctccatttg ttgatattgt
    10561 tgcagttttg gtaaacccag aacaatcttt tgttcaactg attattaatg aaattcctgt
    10621 gcagttattg caatttcatg gtgaggaatc ttcagagttt tgcagacaat ttaataagcc
    10681 gtttatcaaa gcgattcatc ctaagacagc gatccaaatc caaagtgctg ttgatgagtt
    10741 ttttgatgcg agcgctatat tgttggatac gccatcagac aaggaacgag gaggaacagg
    10801 attaactttt gactggaata taattcctga aaatttatcc aagccttaca tattggcagg
    10861 tggtttgaat gaatctaaca ttttagaggc aattaccatg tgtcatcctt atgcagtgga
    10921 tgtgtgcagt ggtatagaag cttcacccgg agtaaaagat catttgaaaa tgagccgatt
    10981 tataaaggca atatggggat agtatgatta aaaaagagct tccagatgag tttggccatt
    11041 ttggcccata tggcggtatg tttgtagccg atacattagt tcatgcttta aaacaattag
    11101 agcatgccta taccaaatat cgtaatgatc aggattttct gtctgagtta cacacagaat
    11161 taaaagatta tgtggggcgg cccaatcctc tataccatgc tgtacactta agtaaaaaga
    11221 taggcggtgc acaaatttat ctcaagcgtg aagatttaaa tcataccggc gctcataaaa
    11281 tcaataatac cattggacaa gccttactcg ccaaacgcat ggggaaaact cgagtgatcg
    11341 cggaaacagg cgcaggtcaa catggcgttg caactgctac agttgccgct aaatttgggt
    11401 ttcaatgcgt tgtgtacatg ggatcagaag acattaagcg tcaatccagt aatgtttata
    11461 gaatgaaatt gttaggtgct gaagtggtgc ctgtcacttc aggatcaaag acgttgaaag
    11521 atgctttaaa tgaagcattg agagattggg tgagtcatgt cgacgacacg ttttatatta
    11581 ttggtactgt tgcaggtccg catccttatc ctcaaatggt aagagatttt caagcgatta
    11641 ttggtgttga agctcgagct cagcatatgg aaaaaacagg ccgtttgcca gatgctttgg
    11701 tagcttgtgt cggaggtgga tccaatgcaa taggcttgtt ttatcccttt ttgaatgatc
    11761 agtctgtcat gatttatggg gttgaagcag gaggtaaagg catagaaacc ggtgagcatt
    11821 cggcatctct gattgctgga aaacctggtg tgttacacgg taatagaacc tatttattgt
    11881 gtgatgagta tggacaggtg aaagacactc attcagtatc agccggtctg gattatcctg
    11941 gagttgggcc tgaacatgct tatttaaaag atactggacg ggtcatttat aaagcaataa
    12001 atgattccga ggccctggat gcgtttcgcc tgttaaccca tacggaagga attattccag
    12061 cattagaatc tagccacgcg gtagcctatg ccatacaatt ggctaaaacg atgtcaaaag
    12121 aacaaagcat catagtcaat ctatctggtc gtggtgataa agatatgcat acagtagctg
    12181 ctattgacgg tataacaatt taattttgat aaattattca ggtacaacat gaaccgtatt
    12241 gataagactc tggaaaaatt aaaagccaat agaaaaaaaa tgttaagtcc ttatattact
    12301 gcaggcgatc catatcctga gttaacagta agtttaatgc atcaattggt taaatcaggg
    12361 gcagatgtat tggagctggg aatacctttt tcagacccta tggcagaagg tcctgttatc
    12421 caaagagcaa tggaacgagc tttggcgcat tcaattcatt gtgatgacgt attgaatatg
    12481 gttaggcaat ttcgcaaaac ggataccgaa acgcctgtga ttcttatggg atatttaaat
    12541 cctatagaac aatatggcta tgatcttttt gctcaacagg cagtggaggc tggggttgat
    12601 ggaacgatat tagttgattt gcctcctgag gaagctgatg gagtatcgcg agtttggcaa
    12661 aaacatggtt tgtacagtat ttatttatgt tcgccaacca cctcagctga aagaatgaac
    12721 tttataaatc agcatgccaa tggttatctg tattatgttt cattgaaggg agtgactggt
    12781 tctgacgcgc ttaagttgcc agaattaaaa gctcaatatc tacaacgaaa ggcacaatca
    12841 aaattaccgc ttatggttgg gtttggaata aaaactccgg aaatggctgc acaagttgct
    12901 gaatttgctg atggagtcat tgttggggcg gcactgataa atgaaatcat tgaagcctat
    12961 gaggccaaga aagatccgct acaagcaagc ggggctctat tgagttccat gaggcaagct
    13021 atcgataata ttggaagtat ggtatgacag aacaaactga aaaaagagct cattttatta
    13081 gacaactcat tatagatgat ttggcaaatg gaaaacatca gggaatcgtt actcgttttc
    13141 ctcctgaacc aaatggatat ctccatgttg gacatgccaa atctatttgt cttaattttg
    13201 gtttggcaga agagtttaag ggaaaatgct ttttacgttt tgatgacacc aatccaatta
    13261 aagaagaaga agaatatgta aacgcgatca ttgatgatgt tcgttggttg ggatttgaat
    13321 ggtgtgccat gacgcattcc tccgattact atcatgagct gtatgaatta gccatttatc
    13381 ttattaagaa agatatggct tatgtcgata gcctgagtat ggatgaaatt cgtgcttttc
    13441 gcggtactct acaagagcca gggcgcgaaa gcccttatag aaacagaaca atcgaagaga
    13501 atctggattt attcgctcgg atgaaagctg gtgagtttcc tgatggaact catgttttga
    13561 gagcaaaaat agatatgaaa tcaggtaatt taaatatgcg tgatccggtt ttatatcgta
    13621 tacgtcatgc ccggcaccag cgtacaggag atgaatggtg tatttatccc atgtatgatt
    13681 atgctcatcc tatttctgat gccctggaaa aaataacaca ttctttatgt acgttggaat
    13741 tccaggatca tagacctttg tatgattggc tggttgataa tttacctttg cctgccaaac
    13801 cagtacaaac cgaatttgcc agattaaatt tatcccatac tgttacgagc aagagaaaat
    13861 tacgtgaatt ggttgaaaaa aaaatcgtta caggatggga tgacccacga ttgcccacat
    13921 tacgtggtat gcgcaaaagg ggatacccac ctgctgcaat tcggcaattc tgtgaaataa
    13981 ttggcatatc aagaagtgat tcagtgattg atatgagttt acttgaggaa tgtgtacgcg
    14041 ctgaatttaa taaaactgca aaacgggctc tctgtgttat ggatccctta aaagttgtta
    14101 ttgtaaatta tccggaaaat aaagtagaac agcttcaagc agctttctac cctcaaaatc
    14161 ctgagtcaga gagtaggaat ttaccctttt cccgtgaaat ttatattgaa aagtctgatt
    14221 ttatggaaaa tccgcctaat aaatatttcc ggttatctcc tggcgctgaa gtacgcttac
    14281 gtcatgccta tgttataaag tgccagcaag ttatccgtaa tgaccaaggc gaaataacag
    14341 aattgcattg cacttatgat gaaaatactc tcggcaaaaa tccggaggat cgcaaggtga
    14401 agggtgtcat tcattgggtg tcttcatcac atgcctatcc tgtcactatt tatcaatacg
    14461 acaggttatt tacagatccc aatcctgcac gggaggatga ttattttcag tttctgaacc
    14521 ataactcttt acaaaccctc cagggatttt gtgaaccggc aatggctgag caggcagaag
    14581 gagatgtgtt tcaatttgag cgtttaggtt attattgcgt caattcagtg gctgagggct
    14641 gtgttcaagc ctttcatcgg gtagtggatt tgaaggatac ttggggtaaa gtgagttgag
    14701 aggcagacat gttacattta tataattctt tgaccagaaa aaaagaacca tttgtttcct
    14761 taaggcccgg aaagataggt atgtatgttt gcggaattac tgtgtatgac cactgccact
    14821 tagggcatgc ccgttcaatg gttgcctttg atgtaatggt tcgttatctg cgctcacaag
    14881 gattcgatgt aacttatgta cgcaatataa ctgacataga tgataaaatc attgcaagag
    14941 cctcggagag aggtgtatct attgatgaat tgaccgcaca atatattgat gcgatgaata
    15001 atgatactca tgcattaaat attttgccac ctgaccatga gccaagggca accgggcaca
    15061 ttgaaactat cattcgcttg attcaacgtt tgcttgaaaa aggtaatgcg tatgtcagtg
    15121 agaatggcga tgtgtgctat gaagtagata cctttcccga atatgggaaa ttatcgcata
    15181 aggatattga aggcttggtt tcgggttctc gcgtagagat tgtaaaagag aaacgttcac
    15241 cgcttgattt tgtgctatgg aaaaaagcaa aaccgggtga acctagttgg tcttcaccgt
    15301 ggggggaagg gcggcctgga tggcacattg agtgttcagc tatggctatg catgaattag
    15361 gtgaacaatt tgatattcat ggaggtggat tggatttgca attcccacat catgaaaatg
    15421 aaattgctca aagtgaagcg gctacgggta aaccttttgc taattattgg ctgcatgtcg
    15481 gtatgttgca ggtcaatggc gaaaaaatgg caaaatcaat agggaacttt tacactattg
    15541 ccgatgtttt aaaagagcat catccggaag tcatccgcta ttttctatta agtagtcatt
    15601 atcgcagtcc tttgaattat tcagaagaaa atttgttaaa tgccaaaaaa gcccttatca
    15661 ggctttatca agcggttaaa gatgttccgc cacaaactgc agatagtaaa ctggatgagt
    15721 attggcaaga gcaatttaat caggcaatga atgatgattt caatactcct gttgcattat
    15781 cggttctttt ccaattggct catgaggtaa acaaaagtaa ttcacctgca ttggttcata
    15841 ccctgaaaat ccttgctggt attcttggat ttttacaaaa agatcccgaa tcattcctgc
    15901 aatccggctt ggcagaggaa gaaaaattgg tcatagaaca actcattgca gagcgtcttc
    15961 aggcgcgagc ggaacgcaat tgggcaaagg cagatcagat tagagctgat ttattgagca
    16021 aaggcataga gctggaagat ggtgcaacag gaacaacatg gcggaggatt gctgaataat
    16081 tgttccttct atgcggttct cttgaatcag aatggacttc tatcccatac ttaatatagg
    16141 ttgcaattac cttttcagaa attttaaaac aggtatattg gctacattaa ttgctattta
    16201 ttgaaggggt tgttcattaa gataaattcg aaattacatt tgaattcgta atgaatataa
    16261 aaacatatct aaaaaaaaat gatttaacct tacgatttgt tggatggttc tttttaataa
    16321 actcattcat attttggctt attggcttgg ggtatttaaa gtccatttta ttaaatgcct
    16381 ctctgtttaa aaattatatc gcggattatt caagtctatc aggaaaaatt ctcattctgg
    16441 ttttctcagt tttaaattat tttacttata tgatgttttt agcttttgtc cctgcaatac
    16501 caatcataat aatggcattt tttttacctt ataaacgaat aatttggatt atcagtgttg
    16561 ttgcggctac cacaagtctt atttttttat ttgttgatag tcaagttttt ttaatgttta
    16621 agtttcatct taataaaaca atattatcgt ttgtttttaa ttcagattgg agtgtcattt
    16681 tcactttttc cagatatgaa ctgattttgt ctttttttat gattgttttt atactctttc
    16741 tggagtctat tgttgcctgg ttggtatgga aaaaaatagt tttaaaagaa cgtttgaaaa
    16801 ttggtaaaac tatttcatta ttttggcttg gtggggcatt aataagttat tttatattgc
    16861 tcttatcgat agctcaaaat aataatttat ttgctcagca aatacctaat ttgccccttt
    16921 ataatcaact cattgcctat actatccctg ataaaaacgc ggatgatata ttacgccgct
    16981 acagtgagca ccattttaca cagcctcttt tccccaatta tccattgcat tatcccttac
    17041 atcccatgca atgtaagatg cctgaaaaac cttataacat tatcctcata atggtagatt
    17101 cacttcgcta tgattcccta agggcaaaat atatgccgaa tacggcacaa tttgcaaaaa
    17161 gaagttggca gttttctcag catatgagtg ctggaaatgc cacacaacca ggattgtttt
    17221 ctttatttta ttcgattccc agtaattact cgacggcagt cctggaacaa aaaaaatcac
    17281 cagttttaat tgatttatta ttacaacatg gttatcagac aaaaattatt tggtctggtt
    17341 ctatggctcc actgccatta gatcagacta tttataaaaa aattgccaat ctcaatatca
    17401 atggagcacc aatagatgat accggagaca aggacagata ttccaccagt gaagctatcc
    17461 aatttttaac agatcctaaa acaaataacc cattttttct tcatattttt tacaatgcgc
    17521 cacatgacta ttgtagatat caaagttttc cgcagttata caaacccgca atagaagaat
    17581 gtgtacgaat tggaatgaca aatcatgatg atccagtacc atactacaat cgttatttga
    17641 atgcagtcac atttattgac caggaaattt cgaaagtatt aggggttatc gaaaaaaaag
    17701 gttatttaaa aaacagcatc attataatta cctctgatca tggccaagaa tttaatgata
    17761 atcgtcaaaa ttattgggga cataccagta attttacccc tattcaacta cacgtcccct
    17821 tgattattca ttggccaggg gagttagctc gtcgttttaa ttatctcacc agcagctatg
    17881 atattgtccc tacaatatta cagcgtttat ttgcttgtaa aaatcctgtt tctgactaca
    17941 gcatcgggta taacctgcta attgaaggca atcgagcctc atttctattg gtaggaagtt
    18001 atattaatat gggaattttt gaagcagatc gcgccacagc attagaaacc tccggaagaa
    18061 ttacaattac tggtatgaag gcagagcctc ttgacgatgc ggtacccaga acggatgtta
    18121 ttacccaggc attggatttg atgcgcaagt attatgggaa ataaagattg tctttaaaaa
    18181 taaattggca attaaccttt ttattctcaa tttaggtcat acagcggata tctttaaatt
    18241 tgtaggcaaa atatggctat aattatatat aatttgcttg ggaattaagt aatggcaaac
    18301 caaacacgtg aaaatttaat taataaggca aagaacgggt ccgaatctat agcgttaatt
    18361 tattctccaa tagaacttca tgaatatatt gttgaaatta tttctaaaga ttcagatcac
    18421 tttatccata aaggaaagga aatatgtcat tttaattcga ttgatgaagc ggtgtctcgt
    18481 gctaccgaat taggggcaaa agagtttttt ctttgtgcag ataatacata tgatgaatgt
    18541 ggttct
//
LOCUS       DATNSN010000051        18276 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.051, whole genome shotgun sequence.
ACCESSION   DATNSN010000051 DATNSN010000000
VERSION     DATNSN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18276
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.051"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1109
                     /locus_tag="VAH76_001453"
     CDS             <1..1109
                     /locus_tag="VAH76_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386085.1"
                     /translation="LLELFVLINMAQSYDIDEQLKAIMSWLYQFNPILKASNKGLLSF
                     YAELEPKLHSEGHLEKRVETGTDNLLYRTKIFLVSLFVTPFEVFPFSGKTISFWDINN
                     VIFSEGEKIYNQFAPFLMTNKLDILIAIYKKTIEEHIIPCQKNKHIYKWLTHYQSTED
                     WYQLVETGGLSKLDVYWFDPELILHGLAHFRLINKSLGEKIVNFLDELIHTYAQNNNE
                     FQIQLRINILFSRFLKSLDEHQRRKLILTLSLFDPVEAKSKFLTNCIHYVTNRLCQIS
                     MHQLDSSPNFFGTYQCIDSKKLLINKTDVKQVSAILEAFKEMLHSLEERCNPEQLENM
                     LIFLRNISRPILTVAEIEEAQQSARVIDYIGAPT"
     gene            1270..2439
                     /locus_tag="VAH76_001454"
     CDS             1270..2439
                     /locus_tag="VAH76_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2744 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386086.1"
                     /translation="MSNLIELALRNAALDRTKLLSQAQKIEFLNGYDPRFVEGLISLE
                     MIDDIDEYVASLMYVEMTDKYTQEEKEQLTDTILTINNDRLISCQYRFPQLYQKLMQD
                     QFLSTFFSSSHLLPNIVPGTNNQSYVTVSTNNKMKMESAKLYGHYEYYQCVVALITQV
                     DGSRYFYHLNPNRIFGDYMGSAPLKLSGSEVKDVKNITFILHPRSMVDYREFQRLQER
                     NIVFKTVMLPVGAKSVSLSYDTDKDVLTVNNLEAGQTVTYNNLLQTPQYNRYQYTPEQ
                     YFMTQLYRGQTWAEALGEALDKYIVTRQEGATWWDLKPLFFNRETTKNNMIAAARIKK
                     EVEAGATSFDFTGVSRNGLLNQLLTTAVDYQLEYQRGLQNGVLSAEDLTTSAALR"
     gene            complement(2474..3451)
                     /gene="rsgA"
                     /locus_tag="VAH76_001455"
     CDS             complement(2474..3451)
                     /gene="rsgA"
                     /locus_tag="VAH76_001455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HEO1386087.1"
                     /translation="MSKRRINKQQSARIAKIQKNYHESMGNNSDNLADGLVITRFSRH
                     AEIEDNQGKRIRCSIRPNLDTLVAGDRVIWQIEGEKQGVVVSIYPRGSLLARPGQKGL
                     KKPVAANITQLVVVIAPKPEISWPLLDSYLIMAEILQLHVVIVLNKIDLPCQTLQQRL
                     MTDYQLLNYPVIMASNQIPDTIVPLKQALNNQISVFVGQSGVGKSSLISSLLPHEENI
                     AIDKISMVSELGKHTTRNSRYYHLPSGGALIDSPGVREFSLWDIDCSMIAQGYKEFKP
                     YLSQCKFRNCTHIDTPQCAIIDALNKGKISTQRYENFIKLCAQFNSLKE"
     gene            3514..4746
                     /locus_tag="VAH76_001456"
     CDS             3514..4746
                     /locus_tag="VAH76_001456"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEO1386088.1"
                     /translation="MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRRYRQVGR
                     DFPVFLHPETKEEYALARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDE
                     QGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFRVANETRLLM
                     YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPN
                     PYQYHQEIDTGIHSLMALRASSELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEE
                     GTKLIRALCTRLRIPNDYRDLAVTVARAHLNIHRVCELRPNTVVKLLEQVDAFRRPQL
                     FHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVKPQTFIVQGYEGKAIKEAMHQ
                     SRVACVERIMTSWKSNEK"
     gene            4736..5299
                     /gene="orn"
                     /locus_tag="VAH76_001457"
     CDS             4736..5299
                     /gene="orn"
                     /locus_tag="VAH76_001457"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEO1386089.1"
                     /translation="MKNNQNLIWIDLEMTGLEPEQDRIIEMATIVTDPQLNILAEGPV
                     IAVSQPKILLDSMDAWNTKQHNQSGLVKRVLESNVSESQAEQLTIEFLKQYVDKGKSP
                     MCGNSICQDRRFLYKYMPELAAYFHYRNLDVSSLKELVLRWRPELMNGVVKESKHLAL
                     DDIKDSINELIYYRQHFINLPEVKNDK"
     gene            5289..5951
                     /locus_tag="VAH76_001458"
     CDS             5289..5951
                     /locus_tag="VAH76_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO1386090.1"
                     /translation="MTNRERLELPNGAKKLLLHSCCAPCSGEVMEALIFSEIDFTIFF
                     YNPNIHPLQEYEIRKEENIKFAQKHNIPFIDADYDKENWFARAKGLEWEPERGKRCTM
                     CFDMRFERTALYAYEHDFPVISSSLGISRWKDMNQINDCGVRAASHYPDLIYWTYNWR
                     KNGGSERMYEIAKREAFYKQEYCGCVYSLRDTNAWRKQNTRDRIKIGLNYYSESKNEG
                     EL"
     gene            5948..6871
                     /locus_tag="VAH76_001459"
     CDS             5948..6871
                     /locus_tag="VAH76_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO1386091.1"
                     /translation="MSKHSHDGAHHHEHGEVTYDRAFIIAITANGLFVVMQIIFAYLA
                     NSTSLLADAFHNLGDVLGLILAWVATGLMKRKPTHKATYGFKKTSILAALANGALLVF
                     TCGIIATDAMYKLFSPTEIQAVSVMIVAAIGIVVNFSTALLFLRGSDDLNIRGAYLHL
                     FYDALISVGVVLSAGVLYLTGWLWIDPVVGLLIALVILKGTWSLFADSFRLIIDGVPR
                     DISWIKVSDFLLAIPGVKSIHDLHIWAISTKENALSVHLFMPEEPLSDDLRRELVEKL
                     KHDFSIQHVTIQVEKTEAECNDACHNPRIII"
     gene            complement(7007..8194)
                     /locus_tag="VAH76_001460"
     CDS             complement(7007..8194)
                     /locus_tag="VAH76_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEO1386092.1"
                     /translation="MIRVLFAFIILLLSVFLGIQLNKDPGYVLIAINNWTIETTLWVS
                     VFGLFFLFFLIYGLVQLCNKITRMPGMVNKWRSRRLAQKAQATTRKGLIEYSEGYWQK
                     AKNHLIQALPNTDTPLLNYLTAARAAQKMGDSKLRDHYLREAQQSMPEAKIAVELTQA
                     QLQLANHQWEQALATLKHLQDLAPRHPYVLKLLMNLYEEIKDWQQLIALLPDLKKNHI
                     ISDQAFEQLQKNTYLQAMIDLAKHNQSDGITKLYQTLPKTLVNDTAITAEYARFLIRN
                     RDYDQAEYHLKRCLRKELDNRLIELYSLLPCEENQLLFAESLLKKNPHSAALYLCLGR
                     MCLSRHLWGKAKHYLEQSIELNPTPVAFIELGKLHEKLNDPLSSGSCYKKGLELITKD
                     ELS"
     gene            complement(8197..9321)
                     /locus_tag="VAH76_001461"
     CDS             complement(8197..9321)
                     /locus_tag="VAH76_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEO1386093.1"
                     /translation="MANSNEEQKKTIQPQQPPKVEPDINQQMKASFFGSKYLSLIISL
                     TSLLAFLAIIVSIYSIQLEKQFQNHQLVENKNLTDELGRIKAEQNTVQKLLDNNANHL
                     QQIQSDLTSKMDSLNKELQTAMKQKLYQNNDWLLLKARYYLELAQINVHWSDNFNTSV
                     ALLQQADALLKGMNIPKIFTIRQIIAKEIAQLKSISVVDITGILSQLDAAQAAINNLS
                     IQSVVDQQEVLNHITKSEESGKTGWRNRLQDSVNFLEKLVVIRRHNENIQPLISPLYE
                     SAIKENIRLNLQEAQWAILNNNPAVYQFALNQAIANLKRVFNESSQNTDALIKQLSTL
                     QNVKLTQEKPVVGQAIPLINQMIDNKELLVDQANSKKGEN"
     gene            complement(9325..10077)
                     /locus_tag="VAH76_001462"
     CDS             complement(9325..10077)
                     /locus_tag="VAH76_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEO1386094.1"
                     /translation="MTKSLNGLRILNTRPREQAQVLNKKIIESGGIPIDCPTMEIMAS
                     ENNWINNLPDLTLVNQAIFVSANAVRYFMMELASKSINWPNSIRVAAIGKGTSRVLNE
                     FNIQVSDIPELPDSEHLLTLNSLQQIRNQTILLVKGEGGRQLIEEYLMLKGAKLCLLS
                     VYKRVMPTIDKEFLNSLWRDDLADIILLTSEQSIHNLFKMFSIEGQMWLQNKPCLVIS
                     DRLAKVASLFGIKKILISHPERMIGTLFDYKD"
     gene            complement(10074..11003)
                     /gene="hemC"
                     /locus_tag="VAH76_001463"
     CDS             complement(10074..11003)
                     /gene="hemC"
                     /locus_tag="VAH76_001463"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEO1386095.1"
                     /translation="MSAKIIRIATRQSPLALWQANHVREMLVKQWPNLSIELLPMITS
                     GDRFLKDKLLSAGGKGLFVKELEEALLDKRADLAVHSTKDMPAQLPDGLLLAAICKRD
                     NPFDALISPQFKSLDALPKNAIIGTSSLRRQSQLLAYNPNLQIKTLRGNIHTRLSKLE
                     SGEYQAIILAAAGLERMGLAHHITQLIPDDIMLPTCAQGALCIECRTDDLEIQELIHG
                     LNDPISALCVHTERRVNAKLGGNCHIPFAVYCTITEEKRLLLRAKVLNMDGSQMIDDE
                     QQGKIAEAEVIADRCTESLMTKGAMSLLSTIPS"
     gene            11393..12682
                     /locus_tag="VAH76_001464"
     CDS             11393..12682
                     /locus_tag="VAH76_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO1386096.1"
                     /translation="MSQLKRIVKHLDESMQRSLSNSAHQLVAVGAIAFVGFPLFYVIW
                     AFWLPQPYENLPLRLIGSFLGLGLMLTPYWPLKWKQYLSWYWFLTLLFTLPYFFTFLF
                     LMNQASVISAMSLLCGVFLLVLLVDLLSLSIVLILGFSLALVSYYLVSPQMYFGEEHI
                     QMTLVIIFTIIAGSTLNYKTAMLQQQKLAGMAAAAGMIAHELRSPLLGIKSGAQALVQ
                     YLPRLIDGYQLAKKQELISSPLRDRLLLQLGGVSNRIVNEIDYANTIIDMLLVKASRD
                     NILQNCILETCSIAECLSEAITRYPFQSPRERTLITWTGDFQFKGSKLLMQHILFNLI
                     KNALYVIAMAQRGEINIWTESGDKYHYLYFKDTAKGMSKQQLNRLFNHFYTTTFMGTG
                     IGLSFCKLVMNAFGGDIVCESKEGEYTLFILSFPGSV"
     gene            complement(12770..13333)
                     /locus_tag="VAH76_001465"
     CDS             complement(12770..13333)
                     /locus_tag="VAH76_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEO1386097.1"
                     /translation="MANSQEFYQPPVDELKRNWGWILGFGIFFLILGCVGLGMVVGLT
                     LVSMFFFGALLIVGGISHIIDVFKYKEWKGMIWQALIAVLYIAAGCIVLYDPFLASTL
                     ITAFLAAVLIIIGLTRMIMAFALKDSKGWGWLLLAGITAIILGILIIMQWPVSGLWII
                     GLFIAIELIVTGWTYIFIAISVKMAKL"
     gene            13615..14640
                     /locus_tag="VAH76_001466"
     CDS             13615..14640
                     /locus_tag="VAH76_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386098.1"
                     /translation="MQHFSIPTCYFPSTAVFVDDSRDFLLNFVLQLDEGLAYRVFDSP
                     FEALDCIKQKRCELELLSERCLSEYTEAKNCPLTNHTINLDLAAIHAEVYNSRRFSEI
                     SVVVVDYAMPGMDGLEFCRRIEDTNIKKILLTGQADEKLAIAAFNEGLIHRYIKKSDP
                     DVASLITQSIHDLQLQYFQSMSDMIVRMLSVTSPNCLHDKKYAELFWRLCREKGIVEF
                     YLADNSGSFLMLDDDANISFLIVKNEADMQLHYDLALDNGASGDVLDQLHNGEKIPCF
                     WQSNLVTPQWNEWSNCLVPANRFVSDETYFYAYVQGAVLFDVRLNKILSYHQYLEELD
                     AEEMFLN"
     gene            complement(14722..15972)
                     /gene="cqsA"
                     /locus_tag="VAH76_001467"
     CDS             complement(14722..15972)
                     /gene="cqsA"
                     /locus_tag="VAH76_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing autoinducer CAI-1 synthase"
                     /protein_id="HEO1386099.1"
                     /translation="MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVN
                     KSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLN
                     NSSFLLSQCENKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
                     GVKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLTAYADIARKFK
                     CLLIVDESHSLGTHGSQGKGMVAKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEY
                     IRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADFLREALSCEGINIG
                     SSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSD
                     LLRVIECLAKLARKRPDIPLFKGN"
     gene            16161..16589
                     /locus_tag="VAH76_001468"
     CDS             16161..16589
                     /locus_tag="VAH76_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEO1386100.1"
                     /translation="MRLNIALILSFFSIMLFAMDEVDEQQILQRIQPVGKVRVQEDLG
                     NNIEAEATKATVVDSKSKKEPGQDIYDKYCSVCHKDGLAGAPKFQSEADWKPRLASKK
                     IDDLLASAIKGLNAMPAKGTCMDCSDAELKAAIQYMLPKS"
     gene            16586..17077
                     /locus_tag="VAH76_001469"
     CDS             16586..17077
                     /locus_tag="VAH76_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEO1386101.1"
                     /translation="MKKLTYRKIQSFQAIITVLVIFASFYLEYAVGLQPCPLCLMQRI
                     CVFILLGLMGLSLGTLKKAHIVSLMQFLVACAGLYFSLRQLWLQSLPSDQAPACMPGL
                     DVLIQYFPWQTVAKALFWGAGDCAEVTWTMFGISMPGWAAMYFLFMAIMGLFLFFRTR
                     TIN"
     gene            17139..>18276
                     /gene="tgt"
                     /locus_tag="VAH76_001470"
     CDS             17139..>18276
                     /gene="tgt"
                     /locus_tag="VAH76_001470"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO1386102.1"
                     /translation="MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVN
                     NMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVF
                     SLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
                     KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSA
                     MNTDGIAIGGETVGFDMNTTVEVIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGID
                     MFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENERIQIKKSCFADDLAPVMESCS
                     CYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHL"
BASE COUNT         5369 a         3179 c         3740 g         5988 t
ORIGIN      
        1 atctattaga actttttgtt ttaatcaata tggcgcaatc ttatgatata gatgaacagt
       61 taaaagctat aatgagttgg ttgtatcaat ttaatcccat attaaaggcc tcaaataaag
      121 ggttattatc tttttatgca gaattggaac caaaattaca ctcggaaggt cacttagaga
      181 agagggtaga aactgggaca gataatttat tgtatcgcac taaaattttt ttagtgtcat
      241 tatttgttac tccatttgaa gtttttcctt tttcaggtaa aacaatcagt ttttgggata
      301 tcaataatgt aatcttctca gaaggagaaa agatctataa tcagtttgct ccttttttga
      361 tgacaaataa gttagacatt ttaattgcaa tctataagaa aacaatagaa gaacacatta
      421 tcccttgcca aaaaaacaaa cacatttata aatggctgac acactaccaa tcaactgagg
      481 attggtacca gttggttgag acgggtggtt tatctaaatt ggatgtttat tggtttgatc
      541 cggagttaat acttcatggt ttagcgcatt ttagattgat taataaatca ttgggcgaaa
      601 aaattgttaa ttttttggat gaattaatac atacttatgc tcaaaataat aatgagtttc
      661 aaattcaact gagaatcaat attttatttt ccagattctt gaagagttta gatgagcatc
      721 agagacgaaa attaatttta actttgtcat tgtttgatcc tgtagaggct aaaagtaaat
      781 ttttaacgaa ctgcattcat tatgtgacaa accgactatg tcaaattagt atgcatcagt
      841 tagacagttc ccctaatttt tttggcactt accaatgcat agacagtaag aaattgttaa
      901 ttaataaaac agatgtaaag caagtgagtg ctattttgga agcctttaaa gaaatgcttc
      961 attcgttaga agagcgttgc aaccctgagc aattggaaaa tatgctcatt ttcctgcgaa
     1021 atataagccg ccctattttg actgttgcgg aaatagaaga ggcacaacaa tcagcccgag
     1081 ttatagatta tataggcgct ccgacctgac ttaattaact ggaattatcc taattcatta
     1141 ttgtgcattt tgatctcatc gcgttgataa ggatcgattg tcttggccta gatgagcaaa
     1201 gcttgctcca gcgtgcgaat tgaattataa ttagacaatt tgtctcattt atgcttttaa
     1261 aattataata tgtcaaatct aatagaactt gctttaagaa acgcggcact tgatagaaca
     1321 aaacttttaa gtcaggcaca aaaaattgaa tttttaaatg gttatgatcc caggtttgtc
     1381 gagggattga tatcattaga aatgatagac gatattgatg aatacgttgc ttctttgatg
     1441 tatgttgaaa tgactgataa atacactcag gaggaaaaag agcaattaac tgatactata
     1501 ttaaccatca ataatgatcg attgatttca tgtcaatatc gctttcctca attatatcaa
     1561 aaattaatgc aagaccaatt tttatctact ttcttttcaa gttcgcattt actccccaat
     1621 attgttccag gcacaaataa ccaatcttat gtaacagtaa gcactaataa taaaatgaag
     1681 atggagtcag caaagttata tgggcattat gaatattatc aatgtgttgt cgctttaatc
     1741 actcaagttg atggttctcg gtatttttat catttgaatc caaaccgtat ctttggtgat
     1801 tacatgggta gtgcgccctt gaaattatca ggtagcgaag ttaaagatgt aaagaatata
     1861 acctttatcc ttcacccgcg aagtatggtt gattatcgcg aatttcaaag attgcaagag
     1921 agaaatattg tttttaaaac cgttatgcta ccggtggggg caaagtcggt atcacttagt
     1981 tatgatacag ataaagatgt attgacggtt aacaatcttg aggcaggaca aactgtaaca
     2041 tataacaatc tgctacaaac accccaatat aatagatatc aatatacacc tgagcaatat
     2101 ttcatgaccc aactatatag aggccaaaca tgggcagagg cacttggcga ggcattggat
     2161 aaatatattg ttacaagaca agaaggggca acctggtggg atcttaaacc attatttttt
     2221 aatcgtgaga ctacaaaaaa taatatgatt gctgctgcgc gaatcaaaaa ggaagttgag
     2281 gctggtgcta cctcatttga ttttacaggg gttagcagga atggcttgct gaatcaatta
     2341 ttaacaactg ctgttgacta tcaattagaa taccagcgtg gattgcaaaa cggggtgctt
     2401 tcggctgaag acctaacaac aagtgctgcg ttacgttgat aactgattat ccattttcgg
     2461 cagagctttt tgtttactct tttaaactat taaattgggc acataatttt ataaagtttt
     2521 catatcgttg ggttgaaatc tttcccttat ttagcgcgtc aatgatcgcg cattggggcg
     2581 tatcaatatg tgtgcagtta cggaatttgc attgggacaa ataaggttta aattctttat
     2641 atccttgagc aatcatagag caatcaatat cccacaagct gaattcacga acgcctggtg
     2701 agtctatcaa ggcgccacca ctaggtagat gataatagcg tgagtttctt gtagtatgtt
     2761 tgcctaactc tgaaaccatg gatatcttat cgatcgcaat attttcctca tgtggcaata
     2821 aacttgaaat taatgatgat ttacctaccc ccgattggcc aacaaaaaca ctgatttgat
     2881 tatttaatgc ttgttttaat ggtactattg tgtctgggat ttggttactt gccatgatga
     2941 cgggatagtt cagcagttgg taatctgtca tgagtcgctg ctgaagtgtt tgacagggaa
     3001 gatcaatttt attgagaacg atcaccacgt gtaattgaag tatctctgcc ataatcaaat
     3061 agctgtcaag taatggccaa ctaatctctg gttttggagc aatcactaca accagttgag
     3121 tgatatttgc tgcaacgggt ttctttaacc ccttctgacc gggccttgcc agtaagcttc
     3181 ccctgggata gatgctcaca accacccctt gtttctcacc ttcaatctgc cagattactc
     3241 tatcaccagc taccagtgtg tcaagattag gcctgataga gcaacgaatt cgtttcccct
     3301 gattgtcttc aatttctgca tgcctgctaa atcgagtaat gactaaacca tcagccaggt
     3361 tatcggagtt atttcccatg ctttcatgat agtttttctg gattttagct atacgagcag
     3421 attgctgctt attgattcgt cttttactca tagtgtcaat ctatactata attgatgcga
     3481 ttaataatac agtatgaagg taaatcatat ttaatgaaag tctacttggt aggcggtgca
     3541 gttcgtgatc gattattagg aattcctgtc caggaacagg attgggtcgt cgtcggagca
     3601 acgccggaag aattattaaa aagaagatat aggcaagttg ggcgggattt ccccgttttt
     3661 ttgcatcctg aaacgaaaga ggaatatgca ttagcaagaa cagagcgtaa atcagctcca
     3721 ggatattatg gattcatttg tgattttagt gaatcagtga cattggaaga ggatttggca
     3781 cgacgagatt taacgattaa tgccatggct atggatgaac agggaaattt aattgatcca
     3841 tatcagggac agcgagatct cgaagagaaa ttattacgtc atgtgtcccc cgcttttgca
     3901 gaagatcctg ttcgggtttt acgtgttgcc cgttttgcca gtcggtttca tcatcttgga
     3961 tttagagtag ccaatgagac tcgtttatta atgtattcca tggttaaaca aggtgaatta
     4021 gctcatttga tccctgagcg tgtttggcag gaatggcaaa aaagtcttga agaaaaaaac
     4081 cctgagcaat tcattctttc tttacgttcc tgtgatgcat taagagtgat tttgccagaa
     4141 attaactcat tatttggtgt gcctaatccg tatcaatacc atcaagaaat agatacgggc
     4201 attcattcat taatggcatt acgggcatca tctgagctta gcgaagaacc attagtacga
     4261 tttgctgctt tggttcatga tttaggcaaa gcctcgacac caattcaggc ttggcctaaa
     4321 caccatgggc atgaggagga gggtaccaag ctgattcgag ctttatgtac ccgcttgcgc
     4381 attcctaatg attatcgcga tttggccgtt acagtggcgc gagctcattt gaatattcat
     4441 agggtatgcg aattgcgtcc taacacagtc gtcaagcttt tggagcaggt agatgccttt
     4501 agacggcctc aactatttca taaaatattg attgcttgcc aagcagatgc cgagagttgt
     4561 ggcaaaacag tggtctatcg ccaaactcaa ttatggaatg aaatattatc tgaatgtgtt
     4621 aaagtgaagc cacaaacttt tatcgtacaa ggttatgaag gtaaagcgat taaggaggcc
     4681 atgcatcaaa gtcgtgtggc ttgtgtagag cgaattatga cttcttggaa atcaaatgaa
     4741 aaataatcaa aatttaattt ggattgattt ggaaatgact ggccttgagc cagagcaaga
     4801 tagaattata gaaatggcca cgattgtaac ggatccacaa cttaacattc tcgcagaggg
     4861 acctgtcata gcggtgtctc agcctaaaat tcttctagat agtatggatg catggaatac
     4921 aaagcaacat aatcagtcgg gattggtgaa gcgagtttta gagagcaatg tcagtgaatc
     4981 tcaagctgaa caattaacga ttgaattttt aaaacagtat gtggataagg gaaaatcacc
     5041 catgtgtggc aatagtatct gccaggatag acgtttttta tataaataca tgcctgagtt
     5101 ggctgcctat ttccactatc ggaatttgga tgtcagttcg ttgaaagagc tggtattacg
     5161 ttggcgtcca gaattaatga atggagtagt taaggaatcg aaacatcttg ctttggatga
     5221 tataaaagat tcaatcaatg agctgattta ttatcgacaa cattttatta atttgccgga
     5281 agtaaaaaat gacaaataga gaacgtttag aattacctaa tggcgctaaa aagttattac
     5341 ttcattcctg ttgtgctcct tgttcaggtg aagtgatgga agcgctcatt ttttctgaga
     5401 ttgactttac tatatttttt tataacccca atattcatcc tctccaggaa tatgaaataa
     5461 gaaaagaaga gaatattaag tttgcccaaa agcataatat tccctttatc gatgccgatt
     5521 acgataaaga aaactggttt gctcgagcca aaggattgga atgggagcca gaaagaggca
     5581 agcgttgcac tatgtgtttt gatatgcgat ttgaaagaac agcactctat gcttatgagc
     5641 atgactttcc tgttatcagt agctctttgg gtatttcacg ttggaaagat atgaatcaga
     5701 tcaatgattg cggagtgcgg gcagcaagtc attatcctga tttgatatat tggacttata
     5761 attggcgaaa gaatggcggc tctgaaagaa tgtacgaaat tgccaaaaga gaagcatttt
     5821 acaaacaaga gtattgtggt tgtgtttatt ctttacgaga tacgaatgcc tggcgcaagc
     5881 aaaatacaag agatcgcatt aaaattggac ttaattatta ctctgaaagc aagaacgaag
     5941 gagaattatg agtaagcatt cgcatgatgg agcacaccat catgagcatg gggaagtgac
     6001 ttatgacagg gcttttataa ttgcaattac tgcgaatggc ctttttgttg tgatgcaaat
     6061 tatttttgcc tatttagcga attctaccag cttgctagca gatgcttttc ataatttagg
     6121 tgatgtatta gggctgattt tagcatgggt agcaaccgga ttaatgaaga gaaaacctac
     6181 ccataaagca acgtatggat ttaaaaaaac ctctatttta gcggcattgg ccaatggagc
     6241 gttactggta tttacttgcg ggatcatagc gacagatgct atgtataagt tattttcacc
     6301 gactgaaatt caagctgtct ctgtgatgat agtagccgcg attggtatag tcgtgaactt
     6361 ctctacagcg ctcttgtttt tacggggttc cgatgatctc aatattcgag gagcttactt
     6421 acatttattt tatgatgcct tgatctcggt aggtgttgtt ttatcagcgg gtgtattata
     6481 cctgacgggt tggttatgga tagatccggt tgtcggacta ttaattgcat tagtgatttt
     6541 gaagggcact tggtcactat ttgctgatag tttcagatta attattgatg gtgttccccg
     6601 tgatatatcc tggattaaag tcagtgattt tctcttggct atacctggcg ttaaatctat
     6661 tcatgattta catatttggg caatcagtac gaaagagaat gcactttctg ttcatttatt
     6721 tatgccagag gagccattat ctgatgattt gcgtagagag ctggttgaaa aattaaagca
     6781 tgatttcagc attcagcatg tcactattca agttgaaaaa acagaagcag aatgcaatga
     6841 cgcttgtcat aatcccagga taatcattta attagggcag gatttacgac ttctaatctc
     6901 ttggttaaaa aatgggttta gtttattcat taattgtatc tacacgccct cattaaaaac
     6961 atttttgtct tgcatttcga gttatctctt tcagttttta aatcaattag gataattcat
     7021 cttttgttat caactccagg ccttttttat aacaagagcc actggataag ggatcattta
     7081 gtttttcatg aagtttgcca agttctataa atgctacggg cgtggggttt agctcaatag
     7141 actgttcaag ataatgtttg gcttttcccc ataaatgccg cgagagacac attctcccta
     7201 aacataagta caatgcggct gagtgaggat tttttttgag taatgattca gcaaacaaca
     7261 attgattttc ttcacaaggg agcaggctat aaagctcaat taaccgatta tccaactctt
     7321 tgcgaaggca tcgctttaaa tgatattctg cctgatcgta atcccgattc ctaatcagaa
     7381 agcgggcata ttctgctgta attgctgtat cgttaaccag tgtttttggc agcgtctgat
     7441 acaatttggt aatgccatct gattgattgt gttttgccaa atcaatcatg gcttgcaaat
     7501 aagtattttt ttgtaattgt tcaaaagctt gatctgaaat aatgtggttc ttttttaaat
     7561 caggtaaaag agcgatcaac tgctgccaat cttttatttc ttcataaaga ttcattaata
     7621 atttcaagac ataaggatgg cgtggtgcta aatcttgaag atgctttaat gtggccaatg
     7681 cctgttccca ttggtgattg gccaattgta attgcgcctg ggttaattca acagcaattt
     7741 tcgcttcagg catagattgc tgtgcttccc taagataatg atcgcgaagc ttgctatccc
     7801 ccattttttg agcggctctc gctgctgtta aataattaag taaaggagtg tcggtgtttg
     7861 gcagagcttg tattaaatga ttttttgctt tttgccaata gccctcgcta tattctatca
     7921 gtccctttcg agtcgtggct tgagcttttt gtgccaagcg tcgtgagcgc catttattga
     7981 ccatgcctgg cattcttgta attttattgc aaagctgaac caggccatag attaaaaaaa
     8041 acagaaaaaa caagccaaaa acagagaccc ataaggtggt ttctatagtc cagttgttaa
     8101 tagctattaa gacataacct ggatccttat tgagttgaat accaaggaac acagagagca
     8161 gtaaaataat aaaagcaaac agaactcgta tcattcttag ttttctcctt tcttgctgtt
     8221 tgcttgatcg accagcaatt ctttattgtc aatcatttga ttgattagcg ggatggcttg
     8281 tcctacgaca ggtttttcct gagttaattt tacattttgc agagtggaca attgcttaat
     8341 caaagcgtcg gtgttttggg aagattcatt aaatactctt ttaagatttg caatggcctg
     8401 gtttaaagca aactggtaga ctgcggggtt attatttaaa atagcccact gcgcttcctg
     8461 aaggttaagg cgtatattct cttttattgc agattcatat agaggggaga taagtggttg
     8521 aatattttca ttgtggcgac gaataacaac caatttctcc agaaaattca cactgtcttg
     8581 caaccgattt cgccatccag ttttgccaga ctcttcagac ttggtgatat ggtttagcac
     8641 ttcctgttga tctactacgg attgaataga aagattattg atagccgctt gagccgcatc
     8701 caattggctt aatatccctg tgatatcaac aacagaaatt gactttaatt gggcaatttc
     8761 tttggcgatt atctggcgaa tggtaaatat tttggggata ttcatgcctt tcagtagcgc
     8821 atcagcttgc tgtaaaaggg cgacagaggt attaaaatta tcactccaat gaacattgat
     8881 ttgcgctaat tcaagataat aacgtgcttt taaaagaagc caatcattat tttgatacaa
     8941 tttttgcttc atagctgttt gtaattcttt atttaagcta tccattttgc ttgttaaatc
     9001 actctgaatt tgctgaagat gattggcatt gttatccagg agcttttgga ctgtattttg
     9061 ctctgctttt attcttccca attcatcagt taaatttttg ttttcaacaa gctgatggtt
     9121 ttgaaattgt ttctccaact gaatggaata aatagagacg atgatagcaa ggaacgccaa
     9181 aagcgatgtc aagctgatta ttaaggatag gtatttacta ccgaagaaag aggctttcat
     9241 ttgttggttg atgtcgggct caactttagg tggttgttgc ggttgaattg tttttttctg
     9301 ttcttcattg ctattggcca tggattaatc cttatagtca aataacgtac ctatcatcct
     9361 ttctggatgg ctaataagaa tctttttaat tccaaataaa gaagctacct ttgcaagtcg
     9421 gtcactaatg accaggcaag gtttgttttg taaccacatt tgcccctcta tgctaaacat
     9481 tttaaaaaga ttatgtatgg actgctcact tgttaacagt ataatgtccg ccaggtcatc
     9541 gcgccacaga gaattaagaa attctttgtc gatggttggc atgactcgtt tatagactga
     9601 cagaagacat agcttcgctc ccttcagcat taaatattct tcaatcagtt gcctgcctcc
     9661 ttctcctttc acaagcagaa tcgtctgatt tcttatttgt tgcaatgaat tgagagtcag
     9721 taaatgctcg ctgtcaggta attcggggat atcactaacc tggatattga attcattgag
     9781 tactctggag gtgcctttac ctatagcagc tacccggatg ctatttggcc aattgatgct
     9841 tttggaagcc agttccatca tgaaataacg aactgcattt gcactgacaa aaatggcctg
     9901 atttactaag gtcaaatcag gcaaattatt tatccaattg ttctcagatg ccattatttc
     9961 catcgtgggg cagtcgatgg gtattccacc agactcgata atttttttat taagtacttg
    10021 tgcttgctcc ctgggtctgg tgtttaatat gcgaagacca ttaagtgatt tggtcatgat
    10081 ggtatcgtac tcaataagga cattgcacct ttagtcatta atgactcggt acacctgtct
    10141 gcaatgactt cagcttctgc aattttccct tgttgctcat catcaatcat ttgagaacca
    10201 tccatattaa gtacttttgc tctcaaaagg aggcgtttct cctcagtaat ggtacaataa
    10261 acagcaaagg gtatatggca attgcctcct aattttgcgt tgacgcgtcg ctcggtatgg
    10321 acacaaaggg cagaaattgg atcatttaag ccatgaatca gctcttggat ttccaaatca
    10381 tccgttcggc actcaataca taaagcgccc tgagcacaag taggcagcat aatgtcatcg
    10441 ggtatgagtt gggtgatgtg atgtgctaag cccattcttt ccaagcctgc ggcagcaaga
    10501 ataatggctt gatactctcc agactccagt ttgctgagtc ttgtatggat gtttcctctt
    10561 aatgttttga tttgcaaatt gggattgtaa gccaatagct gagattgacg tctaaggctt
    10621 gatgtaccga taattgcgtt tttggggaga gcatccagac tcttaaattg gggacttatt
    10681 aaagcgtcaa aagggttatc tcttttgcaa atggcggcca acaagaggcc atccggaagt
    10741 tgtgctggca tgtctttggt cgaatgaaca gccaagtccg ctcttttatc caataatgct
    10801 tcttctaact ctttaacaaa taaccctttt cctcctgcgg ataataattt atctttaaga
    10861 aaccgatcgc cagaggttat catgggtaat aattcaatgc ttagatttgg ccattgcttt
    10921 acaagcattt cccgtacatg attagcctgc catagtgcaa gcgggctctg cctcgttgcg
    10981 attcgtataa tttttgcgct catggaatag acggtatatg ttctttgggt aaagatcata
    11041 acattgattc taatttaaaa agaaccccat tcagataata ggtacgcaaa tcttgtcagg
    11101 taattgattt ataaatgatc attcctatcg gtttgagaat tggtttatag tgccaaccac
    11161 caatttcttt gctaaaaaat ttttaaatcc tgtcatttat agttatttaa aaatcaaata
    11221 actatagctt atctaatctt cttattaaaa tttttttgcc tcgaccttgg agctttgtgt
    11281 aagtcctata gagcatatct catgcacaat ccctatattt tatgataggc ttttccggta
    11341 gataaagcgc tgaaaatcag gggcgttttt tagcttgatt taaggataag gaatgtcaca
    11401 actaaaaaga atagtgaaac atttagatga atcaatgcag cgtagtttgt ctaattccgc
    11461 tcatcaattg gttgcagtag gggcaatagc ttttgtgggg tttcctttat tttatgttat
    11521 ttgggctttc tggttgcctc agccttatga aaacctgcca ctccgtctta ttggaagctt
    11581 tttaggtttg ggattgatgc ttactcctta ttggccatta aaatggaagc aatatttatc
    11641 ttggtattgg tttttaactc ttttgtttac tttgccatat ttttttactt ttttattttt
    11701 aatgaatcaa gcaagcgtta tttctgcgat gtctttgttg tgtggtgtat ttttacttgt
    11761 attgctcgtt gatttattaa gtttatccat tgtgctgata ctggggttta gtttagccct
    11821 tgtaagttat tatcttgttt ctccgcaaat gtattttgga gaagagcata tacaaatgac
    11881 attggtgatc atatttacca ttattgcagg ctctacttta aattataaaa cagccatgtt
    11941 gcaacaacaa aaattggcag gcatggctgc tgctgcaggc atgattgctc atgaattgcg
    12001 ctcaccattg ttaggaataa agagcggcgc tcaagcttta gtccaatatc tgccaagatt
    12061 aattgatggt tatcaattgg ctaaaaaaca agagttaata tcttctcctt tacgtgacag
    12121 attattgttg caacttgggg gagtcagcaa tcgaattgtt aatgaaattg attatgctaa
    12181 tacgatcatt gatatgttat tagtcaaggc aagtcgcgat aatattttgc aaaattgcat
    12241 attagagact tgttctatag cagaatgttt gtctgaggcc attaccagat acccatttca
    12301 gtcaccaaga gaaagaacat taatcacatg gactggtgat tttcaattta aaggctccaa
    12361 gttgttaatg cagcatattt tatttaattt aataaaaaat gcgctttatg ttatcgcaat
    12421 ggcccagcgt ggggagatta atatttggac tgaatctggc gataagtatc attatttgta
    12481 ttttaaagac acagcaaaag ggatgagcaa acaacaatta aatcgattat ttaatcattt
    12541 ctatactact acctttatgg gaacaggcat tggtttatca ttttgtaaat tggttatgaa
    12601 tgcgtttggt ggtgatattg tttgtgagtc taaagagggc gaatacactt tatttatact
    12661 gagttttcca ggctcggttt aaaattggtt ttctgttgaa cataccatca ttcgtcttaa
    12721 aagacgtaag gatggtattg agttagagag cccctctgat tctcttctgc tagagtttgg
    12781 ccatttttac tgagatagcg ataaaaatat atgtccaacc agtgacgatc aattcaattg
    12841 caataaaaag cccaatgatc cacagcccgc taactggcca ttgcattata attaatatcc
    12901 caagaataat ggcggtgatt ccggccaata acaaccatcc ccatcctttg gaatctttta
    12961 aggcaaaagc cataatcatt cttgttaaac caattataat aagcaccgct gccagaaaag
    13021 ctgtgattag agtggatgct aaaaacgggt catacaaaac aatgcatccc gccgcaatgt
    13081 acaacactgc aataagcgcc tgccatatca ttcctttcca ctctttgtat ttaaatacgt
    13141 ctatgatgtg acttattccc ccaacaatta ataaggcacc gaaaaagaac atacttacca
    13201 aggttagacc aactaccatt ccaagaccaa cacagccaag gataaggaaa aatattccaa
    13261 atcctaaaat ccatccccag tttcgtttta actcatccac tgggggttgg taaaattctt
    13321 gtgaattagc catatcaata tccttttttg atttcttagc taagataagg tacttattta
    13381 ataatataga cttatattca ataacttgtc taattttgag ctttgatttt aagagcgctt
    13441 attttgaata cttactccaa aaagcccggt gaatataaaa cctaaataat ttggttataa
    13501 taaacaatca tgttacttta ttcacaagtt agccaggttc tgatgatgga gccacaggtt
    13561 atttgaagtt catagaaagg atataacttt ggaacgtttt gctcaggagg agccatgcaa
    13621 catttctcaa tacctacctg ttactttcca agcactgcgg tttttgtcga cgacagtcgg
    13681 gatttcttgc tcaatttcgt attgcagctg gatgagggtt tggcgtaccg tgtatttgat
    13741 tccccttttg aagctttgga ttgtattaaa caaaaacgct gtgaactgga attattaagt
    13801 gaacgttgtc tcagcgaata cacagaagca aaaaattgcc cactaactaa tcataccatt
    13861 aatcttgatt tggctgctat tcatgccgaa gtttataatt ctcgacgatt ttctgaaatt
    13921 tcagtagtcg ttgttgatta tgcaatgcca ggcatggatg gattagaatt ttgcaggcga
    13981 atcgaagata ctaatattaa aaaaatatta ttaactgggc aagcagatga gaagctggca
    14041 atcgctgcct ttaatgaagg attaattcat agatacatca aaaagagtga tccggatgtc
    14101 gccagtctca ttactcaaag tatccatgat ttgcagttac aatattttca aagcatgtct
    14161 gatatgattg tacgtatgct ttcggtgaca tccccaaatt gtttacatga caaaaaatat
    14221 gctgagttat tctggcgttt atgcagggaa aagggaatcg ttgaatttta tctggccgat
    14281 aactcaggta gctttttgat gctggatgat gatgccaaca tcagtttttt aatcgtgaaa
    14341 aacgaggcgg atatgcagtt acactatgat ttggctttgg ataatggagc tagtggagat
    14401 gtattggacc aattgcataa cggagaaaaa attccttgtt tttggcaatc taatttagtg
    14461 acacctcaat ggaatgagtg gtcgaattgt ttggttcctg ccaaccgatt tgtttcagat
    14521 gaaacctatt tttatgctta tgtgcaaggg gctgttttgt ttgatgtgcg cctgaataaa
    14581 attctttctt atcaccaata cctggaagag ctggatgcag aagaaatgtt tctgaattaa
    14641 taggaattct atataaagag cacataattc ttatttgaaa tggtaactgt tcaaatgagc
    14701 tgccaataat tagaagtcct gttaattacc cttaaacaaa ggaatatcgg gtctttttct
    14761 tgcaagtttg gccaagcact caatcactct gagcaagtcc gatttttcgt gatgcgcact
    14821 gatggataaa cgaatcaggc atttattttt gggagtggcg ggtgaacaga acacggctcc
    14881 aaagaggtct tctttttcta actcatccct taaccaaagc gtgttagcct cgctgcctgt
    14941 aactaaaggg actatttgag agccactact tcctatatta attccttcgc atgataaggc
    15001 ttctcgtaaa aaatcagcat tggtttgtaa ttttgctctt ctccattctt cttgagtaat
    15061 aatttctaat gatgcgctaa atcctgctaa atcatgaggc attaaagcag aactaaaaat
    15121 agaaggcaga gatgaatatc tgatatattc tataagttgc ctatcccctg taatgatgcc
    15181 tccacgtcct gataaggctt tagccaggct tgctgttatg atgtctattt ggcttgataa
    15241 tcccagttta gccaccattc ccttgccttg cgagccatgc gtacctaatg aatgagattc
    15301 atcgacgatg agtaaacatt tgaattttct tgcaatgtca gcataggcag tcaatggact
    15361 aatcgtaccc agcgtactat aaatagaatc tactgcaatt attcctggtc catggtgttc
    15421 aattcgtttt tttagtgaat cgactgaatt atgtagaaac ggtataggtt tggctcttgc
    15481 tgcttttaca ccagaccaaa aagacatatg ggcataaaaa tccagataca ctggtgtatt
    15541 gtacttggcc agggtttgta tgagaccaac gttagcgctc cagcctgatt gagtgagcag
    15601 ggccgcagaa taatttaaaa aggaactgaa tttattttca cattggctga gcaaaaaaga
    15661 gctgttattt aaaaatacag cagattgcat ttgaccatta ccatattttt gcatcgcagc
    15721 aatttgcgct ttaattaatt gcggatgttg actgatgttt aaataatcat tgctcgaaaa
    15781 aatcagtgaa tttgcagtcg gggttttgcc tttaagtatg tgtttccctt gccaggattt
    15841 attgactcgt ccctcgtaat atttttccat ggaatgattg atgtactcag gataagcatg
    15901 gttttgatgt ttttcttttc cattgatata ttgagtctcg ttatcaattg tttggtattc
    15961 tgtattgctc atccataact cagattcttt tcccttgtgt gcagtttagc acaatggttg
    16021 atagtggaag gatcttttca gtaaaagtct aagtattaag ttaatgattt tataaatcgt
    16081 tgggcctatt gaaatctttc tggaaaaaaa agtcacttgt cggtatgatt tttaaattaa
    16141 ctattagagg acggataaat atgaggttga atattgcgct gatcttgtct tttttttcaa
    16201 tcatgctttt tgcaatggac gaggtggatg agcaacaaat tttacaaaga attcaaccgg
    16261 ttgggaaagt tcgagtccaa gaggatctgg ggaataacat agaagctgaa gcgactaaag
    16321 cgacggttgt tgattccaag agcaaaaaag aaccaggcca agatatatat gataaatatt
    16381 gctccgtatg tcataaagat ggattggcag gggcgcctaa atttcaatcg gaggccgatt
    16441 ggaaacctcg tttggcaagt aaaaaaatag atgatttact cgcttctgca atcaaggggt
    16501 tgaatgccat gccagcaaaa ggtacttgta tggactgttc tgatgctgaa ttaaaagctg
    16561 cgatacaata tatgttgccg aaatcatgaa aaagcttact tacagaaaaa tacaatcttt
    16621 tcaggcaatt attacagtat tggtaatatt tgcttcgttt taccttgaat atgctgtcgg
    16681 actacaacca tgtcctttgt gcttgatgca acgaatttgt gtatttattt tgttagggtt
    16741 aatggggttg tctctgggaa ccttgaaaaa agcgcatata gtaagcttga tgcagtttct
    16801 ggttgcgtgt gccggtttgt atttctcttt aagacaattg tggttgcaat ctttaccatc
    16861 agatcaagct cctgcttgca tgcctgggct tgatgtatta attcagtatt ttccatggca
    16921 aactgttgct aaagccctat tttggggagc aggggattgt gctgaagtga cttggactat
    16981 gtttggaatt tctatgcctg ggtgggctgc catgtatttt ctgtttatgg cgataatggg
    17041 gctttttctg ttttttcgaa cccgaacgat caattaattc ctggcattaa acgcgtgctt
    17101 tcgttatcat tctttgtttt atcttgtcgt ttctgtgaat gaacactttt tcatgtgaag
    17161 tactgcacca gcattcgggt accaaagctc gtgttacacg tgtggttaca cctcatggtg
    17221 aatttataac cccggtattt atgccagttg gaacccgtgc aggggtcaat aacatgatgc
    17281 cgtcagaact gtctgctgcg ggtactcaaa ttattctggg cggcaatacc tatcatatgt
    17341 tgtgtgcgcc tggcatgaag gtgatagaaa atgcaggggg gatgcatcgc tttatggggt
    17401 ggcatgggcc tatgctaaca gacagcggtg gttttcaggt ttttagttta tcgaagaaca
    17461 aggaaatttg ttctatagat gaatctggtg ctcattttaa attgcctggc agtaagggga
    17521 ttatccatat gactccggaa atgtctctgg agacccagaa aataattggt gccgatatta
    17581 taatggcatt tgatcagtgt actcctgata gctgctccaa agatgaagta agtcaaatca
    17641 tgctgcggac ccacagatgg ctaaaacaat cgattgacta ccatcaacaa tatcccaact
    17701 ctcgatatgg atataaacaa gctttgtttg gcattgtgca agggggagtt tttcaagatt
    17761 tacgtcgaga aagtgctgat tttgtttctg caatgaatac ggatggaatt gcaataggtg
    17821 gagaaaccgt agggtttgat atgaatacta cggtagaagt cattcgttgg gtgcatgagt
    17881 atcttccgga caataagcca cgttatacta tgggggttgg aatgtcacct caagatctgt
    17941 tggatgtggt ggcagaaggt atcgatatgt ttgattgtgt tgcgccaaca cgtaatgccc
    18001 ggcatggtgc cttatattgt ggtaaattgg taagagaagg gaattggcta aaatttcaaa
    18061 gcgaacatga aaatgaacga attcaaataa agaaatcatg ttttgctgat gatttggcac
    18121 ctgttatgga gagttgttct tgttatactt gtcttaatta ttcaagatcc tatttgcatc
    18181 atttatcaaa acaaaaagct aatttattta ctgctttggc aagcatccat aatgttcatg
    18241 ttttgcatga tgtttgtaat cacatgcgtc atttga
//
LOCUS       DATNSN010000052        18063 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.052, whole genome shotgun sequence.
ACCESSION   DATNSN010000052 DATNSN010000000
VERSION     DATNSN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.052"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..358)
                     /locus_tag="VAH76_001471"
     CDS             complement(<1..358)
                     /locus_tag="VAH76_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEO1386103.1"
                     /translation="MHKKIFCQNLLDSALKNSIHAKRKQCLVRFLSDLMDYDTTLSVT
                     EIGKKLTSKTTVKSKIYAAQTFVNNFKLERDIVCIYKSLAHFFWSHAKEIVVLIDWTG
                     GCSEGYHVLEASIAAHG"
     gene            complement(709..2589)
                     /gene="parE"
                     /locus_tag="VAH76_001472"
     CDS             complement(709..2589)
                     /gene="parE"
                     /locus_tag="VAH76_001472"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO1386104.1"
                     /translation="MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSV
                     DEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSD
                     KNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
                     TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQG
                     LTDYLRQTLPEDYLPEEPFAGAFKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGG
                     THVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKE
                     RLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQ
                     GPALPGKLADCLQTDLSQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVD
                     SSQVLASQEIHDISVAIGVDPGSSDLEGLRYGKICILADADSDGAHIATLICALFLRH
                     FKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNVQRFKGL
                     GEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRAADRKLWLETKGNLA
                     EI"
     gene            2723..4531
                     /locus_tag="VAH76_001473"
     CDS             2723..4531
                     /locus_tag="VAH76_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEO1386105.1"
                     /translation="MQNTAKINQLSVAFKSPDKTVLALDKLSFSLNPGETLVLLGESG
                     CGKSLTSLAMMRLLPRAGVYGIDSQINIHGEDILNLPEKMMRELRGRRLAMVFQEPMT
                     ALNPVLTIGEQIAEVLKRHRSLTKEQIKERVILLLNEVEMPQPQMRIYQYPHQLSGGQ
                     KQRVVIAMALACEPDILIADEPTTALDVTIQAQILALLKKIQKKHQMSLLLITHDLGV
                     AKTMADRICVMYAGQVVEQASVKDFFSKVSHPYVQQLLTSLPSFAKRNEKLSIIAGTV
                     PSLESMPSGCRFHTRCIYAFERCHHEEPQLQEVEQRILRCHLYPDIKELPLLDKNKIS
                     WVPSVEKEEIVFTVDDLSVHFIHKKGFLSRKKTIFKAVDGLSFTLSRGKTLALVGESG
                     CGKTTASRALLRLLPAAGGKIRYKDSDVLSMTGSALRRYRKKVQIIFQDPFSSMNPRM
                     TVGEIIAEGMHAQGMKPSYIRKQQKSLIEQVNLPITSLHRYPHQFSGGQRQRICIARA
                     LATEPDILICDEPTSALDVSVQAQILNLLKELQQQRGISYLFITHNMGVVSYIADDVL
                     VMKDGLAVEYGACEKVLQQPEHPYTRQLLNAVLDIE"
     gene            4620..8903
                     /locus_tag="VAH76_001474"
     CDS             4620..8903
                     /locus_tag="VAH76_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386106.1"
                     /translation="MKITRNQLNFKGVKEFGGCLTRNDEQIEFINKEIKLLKELIEFN
                     ILKGIDENKSQSLFDAVIEAVEKAGLDVEHELLIQFIQSTSEIIDSKSTDLPELVSLL
                     TVLIKSSNKYDKSELTRYCRLIDKLEKTDEKTLEQWIKILTILGRNVESVNGDLLFNV
                     QSRLEIHSVLLKDISSLFDYPPYPALDVFSEKLREYSRELKLYIDSFDRDPAGSRAPK
                     VNQEGILIKSREQILNEQFDTSQIQEVIAKIESLVDGSGLSCKGQYELAQQVVFINAI
                     GRDFPLKIANETYTDLTQLGRNKLQELSDFLIMEMRRLDISKEDKLRTQLKLLAVMRE
                     QYFRSTGIFFDTTQMISLLLSLHHQEQNVLMGVSRNENLSAPPAAILAAMQWVLADGG
                     TVDLCFANHGLIAQDYLEKGDRDFFTCLGIPSSVVEANSESGTYQVGGINYSTLSDLT
                     SYRSHAVIEDEELSMDRCGYPVSSYLILNHIDFSNLDDRTFSHFSSLYDYYKGKGRVV
                     RITEIEETVGEVVGQISRFDMDVGYRVFPARKNLQEELNTQSLAERRREEAVKQYFIQ
                     SVSNIQQAVLNQLEEWQAFLHLIYPKSEWRQLDNELFAQRNELIDSLKKQWTKCLEDS
                     DPKKMYPNPYVRRDVFQRLQTRELEKAIQAYEKSVSSIWNLNRDQLKAKTENRFSSDS
                     VNALRCKYLEEVDLSEQIKWSKLSARLKKKEATQEKKRSHRHLESAVEVTGAMLSYHD
                     GDLEPYRLAFVHSQLKLWARDISKEINNSSLPASVKQGLIERANNADNFLALELLLID
                     YESRWLDKEKLSEKFRMQPIISDMLRVYKQAGFEEHKELKILKETYLDSTATQIIHHL
                     DKTLAWAKPENRGPWYWIERSAVKKAANEILVLANGVKQARDSLSKQIAIKKLYRALC
                     KHQAQLEDVWIFSFDHKNTRDLINQTLNTMDSLTVLGRDEGGLDINFIQECKEEAQYY
                     LLGEKFSSVLEDFEKNNSQILTAHSEWQEVKRQLQQKQNESRKVYTLNEMYYVLCKKI
                     NELSISYSLLREPLVQLRGMIRTLWNSFQEKHQDIIDESKYFDFKARKIKEELEGIEG
                     YSVFNVKVKKGYTGFNEYVELIIEGEGTSALFEGFMQYKSRLTELKEVYRQLEFSLKQ
                     NRLELLNLNRIHAKHLPFLKDRIKGNPEVELFPEQCRSKVKEILTLKEWINGQIPENL
                     SQFPENMQNHFLDRNTLKSMDRNTMTMEQIDKIKDEQLKADLTELYNKMRKVDEEQSQ
                     SWFSTVTRRIVGLIPGMFRQEETEDDWRYQFNELQGRSDSILQSLLTKKIMTKYDALV
                     NEMVLIQSNLAQQMDSGNKKIQFLSDKILEEERKTNVVIKRFDNLNDFYDFEAKLKRV
                     KTAPPIASVNEVQIESKNRILSYGEGAFSNPEEEFQTDSVMQPPMVFNI"
     gene            complement(9261..10970)
                     /locus_tag="VAH76_001475"
     CDS             complement(9261..10970)
                     /locus_tag="VAH76_001475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO1386107.1"
                     /translation="MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMP
                     LGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQR
                     EYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLSIESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGE
                     DLIFYSDASDYAANIEQATSLKPPKANQACNETITLVDTPNQKTIDEVASFLGIASNQ
                     TIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFIDEELILKTLKTPLGS
                     IGPIQLNIPVIVDHHALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDP
                     SPDGRGMLHCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVA
                     AAIEQHHDEHGIIWPQALAPFQVNIIPLNGARSQAVKEQAESLYQQLKSHGIDVLLDD
                     RNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVVNFIIEL
                     INK"
     gene            11133..13982
                     /gene="ankN"
                     /locus_tag="VAH76_001476"
     CDS             11133..13982
                     /gene="ankN"
                     /locus_tag="VAH76_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkX"
                     /protein_id="HEO1386108.1"
                     /translation="MVKIMPNLPGLYFLQAYPREEIWRLFVDGRFWSKENGWRGYESR
                     EPGCLNAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGELRTD
                     EPVSFGIPAGRASIKGIEEFLSLVFLTEGEAEFGPGKAGPFGPRFDKNYFKNLNPEQI
                     PDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSYNQEIKTAETLDEKLKII
                     VKHIRMYEVLHPFRDANGRTFVNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVV
                     KEAIFNTVEIIEQSKRKTPITLYGYHSSLEEQTKFRDMLDSPAYEKIKHMDFSDLNPE
                     KLHRKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG
                     KTPAHLAVISGNMAMIDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLS
                     QEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVIQINEPDNSGSSAITLAYK
                     NHKLKIFDELLNSGADISDELLDAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLG
                     SVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNS
                     VLHYVFYSKAENREALANIITEKDKKLINQPNTNGNPPLYNAVVVNDLKMATILLEMG
                     ARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHQALHEMHTF
                     PSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHT
                     NISSPSKFLKNSDANSILERPFKFKNDLKKELDDNPLIAMAQINDLYVQIKNNRIRTP
                     TGYASKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKF
                     FDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW"
     gene            complement(13989..15701)
                     /gene="lem3"
                     /locus_tag="VAH76_001477"
     CDS             complement(13989..15701)
                     /gene="lem3"
                     /locus_tag="VAH76_001477"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector phosphocholine hydrolase Lem3"
                     /protein_id="HEO1386109.1"
                     /translation="MKLRYIINENKLVFTSCNMRDKIITGKKVIFSQSVAKDQTKNLS
                     SFLSERFYSVNQSHNHSIIIGSSLSHQENDIEYDTILDTSGVLVTTDTNGLVNGARIA
                     ITDGLGGGNGDQEEDDEIYRVSHSSCANFLNCDQNMDTTLSLITQPKASDKKQTAPKT
                     LQHTEASMAAFIYQNHPGKGYIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTW
                     TPPSLQALATTANKDALLVRQTRTLAEGDIIISMTDGVWGELKTSLIAQTNDRRDIGV
                     DKEYFKTLFDELTDAPYPSSFDIARIITHRAMSRSLERRKTLIKLINEIEQQHFHEKS
                     VKTINEVLEYFIKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTV
                     GDCSTINVTRIPYHLDELIRGFINYPEKHQILAPLFKARVKSEADLEEAFHRLSLEMV
                     QPEIECPISEAHFEPAFKKETLDKTQAVLTHYFRISTGLDSKKNYQERLNDLSAYLSK
                     ESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFGENQNKLYKAFHKKIELQFLDGEIENK
                     NELK"
     gene            complement(15788..>18063)
                     /locus_tag="VAH76_001478"
     CDS             complement(15788..>18063)
                     /locus_tag="VAH76_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional SulP family inorganic anion
                     transporter/carbonic anhydrase"
                     /protein_id="HEO1386110.1"
                     /translation="FRIYSKRYLKFDFVAAIVVFLVAIPLCLGIALASGAPLFSGILS
                     GIIGGIVVGIFSGSQVSVSGPAAGMAAVVLAAISQLGDFNTFLLALTIAGVLQLIIGA
                     LRAGFVADYVPSNVVQGLLCSIGILLIIKQLPLAFTLSSDFDELKTHLLETTEGFTVS
                     PLLALSQHINEGALIITTLSLAILIYFDITKNKILKEIPAPILVVLAGILLNELFIWA
                     NSSLAQNSPQLVNIPDTNGFFQFFSHLEYPDWSAWTNPKVYLYALVICIVASLETLLN
                     LKASERLDKKRRHSPTNQELVAQGLGNLTAGLVGGIPVTSVIVRTSINIHAGSKTKFS
                     AILHGFFILFAVMLIPGALNKIPLSSLAAILIYTGYKLNKPAIYINIFSQGSDRFIPF
                     IVTVISIIAFNLLAGILIGLAISLFYILKSNSQARIDIIKEIYPNGSTYRLVLPQQIT
                     FLNKAALVAELDTLPRHSQLIIDARYSQYIDKEILELFNELKEELAGSKQISLNFTGF
                     QEHYKIHNYIDFITVTTYDVQTNLTPAQVLNILLEGNQRFLSDNRIHRSNQIDIKYTA
                     KTQHPIAVVLACIDSRVPVETIFDMSFGDLFCVRIAGNVINDDILASIEYACNVVGAK
                     LIMVLGHTRCGAIQSACDGIEKGHITQLLSKIKPAVNAEKETTTERNGKNQIFVNHVT
                     ELNVANTLQNIYKKSDILRTMIDSNEIGMVGAIYDVSSGKVSCKTYTEELSTLDGDEN
                     KLLAQKFETILLEAKAS"
BASE COUNT         5665 a         3293 c         3638 g         5467 t
ORIGIN      
        1 gaccatgagc tgcaatacta gcttccagaa catgatagcc ctcgctacat cctccagtcc
       61 aatcaattaa aacaacaatc tcttttgcat ggctccaaaa aaaatgggcc agactcttat
      121 aaatgcaaac aatatccctt tccagtttga aattattcac gaatgtttga gccgcatata
      181 ttttactttt aactgtcgtt ttggatgtta attttttacc aatctctgta acggataatg
      241 tggtatcgta gtccattaag tcacttaaaa agcgaaccaa acattgtttt cttttagcat
      301 gaattgaatt ctttaatgcg ctatccaata aattctggca aaatattttc ttgtgcatac
      361 aacttccgat tagttttgtc ttggtaaaca aatttgatca tgaatccgta tgcacttcaa
      421 tttcttttcc catctttgtt aatgtacact ttaaaaaagt aactgctcaa ttaaatgaca
      481 ggattcctca gcgcgaaggc gggaatctat ccataaagct agcacaaggg ttgattttta
      541 gttagcttcc cgccttcgcg ggaaagacaa ctcaaataaa taggcttata agcagaaaaa
      601 tttgtatacc ccgtgtacgg ttactatcca tattaccaag gataagatag aaaagtagcc
      661 gataatgact atcgctaaag gggtggccaa ttgctaatgc accttagctc aaatttcagc
      721 aaggtttcct ttggtttcta gccagagttt tctgtcggcg gccctttttt tgttgagcat
      781 catatccata atggcttccg tctgctcttc atcatcaaga gtaagctgta ccagacgcct
      841 tgtattgggg tccattgtgg tttctctaag ctgaatggga ttcatttcac ctaatccttt
      901 gaatcgctgc acattcactt ttgattttgt ggtttgactg agatgttgaa cgattctatt
      961 tttttcctca tcatccaggg catagtgaat cagcttacct gcatcgattc gatagagcgg
     1021 gggcatggct acaaaaacat ggcctgcttt aaccagaggt ttgaagtgtc ttaaaaacaa
     1081 ggcacatatt agtgtggcaa tatgagctcc atcagagtct gcatcggcaa gaatacagat
     1141 ttttccatag cgaagaccct caagatcgct ggagccagga tctacaccta tagcaacaga
     1201 tatatcatga atttcctggg atgccagaac ttgtgaagaa tcgacttccc atgaattaag
     1261 tattttccca cgtaagggta aaatggcttg ataatcttta ttgcgggctt gtttggccga
     1321 ccctcctgcc gaatctcctt caaccaggaa tagttccgct tgacttaagt ccgtttgcaa
     1381 gcagtcggct aattttccag gtaaagcagg cccttggcta acacgtttgc gtgccacttg
     1441 tttggcttgc tttagccgtt tctgtgcgcg ttctattgcg agagctgcta tggcttcacc
     1501 ctggttgcga tgctgattca accaaattgc aaaagcgtct tttatgacat tactgactag
     1561 tcctgaggtt tggcgtgaac tcaagcgctc tttggtttgc cccgcaaatt gcggctcttt
     1621 cattttgatt gataaaacat actggcatga ttcccataga tcatccgctg tcaattttac
     1681 tccacgagga agaaggtttc tcagctcaca aaattcagac atggcttcaa ataatcccga
     1741 acgcaatcca ttgacgtgcg tgccaccttg tattgtcgga attaaattca cataactttc
     1801 atttaaattg ccattggcag agtctgacca ggccaaagcc caatcaacag ttgcttcatc
     1861 acttttgaat gctccggcaa aaggttcttc aggcaaatag tcttcgggca aggtttgccg
     1921 taaataatca gttaaacctt gctcataaca ccagttcatt tcttcattag tgattttatt
     1981 gataaaggtc atggacaagc cactacacaa taccgctttt gctcttaata cgtgagtcag
     2041 gtgttttaca gaaatttttg tagtgtcaaa atatttgcca ttaggccaaa aacgtataac
     2101 agtcccagtc tctctttttt ttgtaacccc ggtttcattt aattcacata atttgtcgcc
     2161 attagcaaag gtcatttgat aaatgatccc attgcgcttt atggtgacgt caagtcgttc
     2221 tgacaaggca tttaccacgg acaccccaac gccatgcaag ccgccagaaa agctgtaatt
     2281 tttatctgaa aacttacctc cggcatgaag tcgagtcata atgacctcga caccacttaa
     2341 gcccagttga ggatgcaaat ctactggcat acctcggcca tcatcctcga cttcaataga
     2401 accgtcttca tgtaaagtaa caataatacg gctggcaaat ccagcaagta cttcatcgac
     2461 gctattatca atcacttcct gagccaaatg gttggggcgt gtcgtgtcgg tatacatacc
     2521 gggacgtcgt tgaacaggct caagaccact taagacttca atcgcttccg cagtgtagtt
     2581 ttctgacatg ggtattcact caaatattaa ttgtgcttat tctatcataa acattctttt
     2641 gctcagagtg gtatggtatt tttattgcta ctatcaccca tctcttgtta gagtgtagtt
     2701 atcattattt gtggcaaatt ccatgcaaaa caccgctaaa attaatcaat tgtcagttgc
     2761 ttttaaaagc cctgataaaa cagttctggc tttggataaa ttaagtttta gtctaaatcc
     2821 aggggagact ttagttttac tgggagagtc aggatgtggt aaatccttaa cttcattggc
     2881 tatgatgagg ttgctgccta gagctggagt ctatgggatt gacagccaaa tcaatattca
     2941 cggtgaagac atcttaaatt tacccgagaa aatgatgagg gagttaagag ggcgcaggct
     3001 tgcgatggtt tttcaagagc caatgacggc tttaaatccc gtattaacga ttggagagca
     3061 gatagctgag gttttaaaaa ggcatcggtc tttgacaaaa gagcaaataa aagaaagagt
     3121 aatattgtta ttgaatgagg tagaaatgcc tcaaccacaa atgagaattt atcaataccc
     3181 gcatcaattg tctggtggtc aaaagcagag ggtggttatt gctatggcgt tggcttgcga
     3241 gccagatatc cttattgccg atgaacctac tacagctttg gatgtgacta tccaggctca
     3301 aatattggcc ttgctaaaaa aaatacaaaa gaaacatcag atgagtttgt tattaattac
     3361 tcatgatctg ggcgtggcta aaaccatggc tgatagaatt tgtgtgatgt acgcagggca
     3421 ggtagtggaa caggcctcag taaaagattt tttttcaaaa gtgagtcatc cctatgtcca
     3481 gcaacttttg acatccttac cttcatttgc caaaagaaat gaaaaactgt ctatcattgc
     3541 aggcacggta ccttctttgg agtctatgcc ttcagggtgt cgtttccata cacgctgtat
     3601 ttatgctttt gaacgttgtc atcatgagga gcctcaatta caagaagttg agcagcgtat
     3661 cttgagatgt catttgtatc ctgatatcaa agaattgcca ttactggata aaaataaaat
     3721 cagttgggtt ccttccgtag aaaaagaaga gatcgtcttc acagtagatg atttatcagt
     3781 tcattttatc cataagaaag gatttttgag tcgtaagaaa accattttca aagccgttga
     3841 tggtttgtcg ttcactctct ccagaggtaa aacattagct ttggttggag agtcgggctg
     3901 tgggaaaaca actgcaagcc gtgctctgtt gaggttattg ccagcggccg gagggaaaat
     3961 cagatataag gatagtgatg tactgtctat gacaggaagc gctttaagaa gatacagaaa
     4021 aaaagtacag attatttttc aagatccttt ttcttctatg aatccacgca tgacagttgg
     4081 agaaatcatc gcagaaggga tgcatgcaca gggaatgaag ccttcttaca tcaggaaaca
     4141 acaaaaatcc ctaattgagc aagtgaactt accaattacc agtctgcatc gataccctca
     4201 tcagttttcc ggtggacaaa gacaacggat ctgcattgcg agagctttgg cgacagaacc
     4261 tgatatttta atctgcgatg agccgaccag tgctcttgat gtttctgtac aggcacaaat
     4321 tcttaattta ttaaaggaat tgcaacagca aagaggtatt tcctatttgt ttataaccca
     4381 taacatgggg gtcgtctctt atatcgctga tgatgtatta gtcatgaaag acggcttggc
     4441 tgttgaatat ggagcatgcg agaaggtact tcagcaacca gaacaccctt ataccaggca
     4501 gctgttaaat gctgtattgg acatcgagta atatttcaaa ttttctttta catttatctg
     4561 ctacatttta tgcagcttat atataatgag ggcattctat ttttcaggga attcagggca
     4621 tgaaaatcac tagaaatcaa ttaaatttca aaggtgtaaa ggaatttggt ggttgtttaa
     4681 caagaaatga tgaacaaatt gaattcatta acaaagagat caagctgtta aaggaattaa
     4741 tcgagttcaa tattctgaag ggaatcgatg aaaacaaatc acaatcctta tttgatgctg
     4801 tgattgaggc agtggaaaag gctggcttgg atgtcgagca tgaactgctc atacaattta
     4861 ttcaatccac atcagagatt attgactcca aatcgacaga ccttcctgag ttggtttcct
     4921 tattaacggt actcattaaa tcaagtaata agtacgacaa atcagaattg accagatatt
     4981 gccgtcttat cgataagctt gaaaaaacag atgagaaaac cttagaacaa tggataaaaa
     5041 tactgactat acttggaaga aatgttgaga gtgtaaatgg tgatttatta ttcaatgttc
     5101 agtctcgcct tgagatccat agtgttttac taaaagatat ttcttcttta tttgactatc
     5161 ctccttatcc cgcattggat gttttttccg agaaattgcg tgaatactca agagagttaa
     5221 aattatatat tgattcattt gacagagatc ctgccggatc cagggctccc aaagtgaatc
     5281 aagaaggtat tctgattaag tcgagagaac aaattcttaa tgagcaattt gacacaagtc
     5341 aaattcaaga ggttattgcc aagattgaaa gtttggttga tggatcgggt ttatcgtgta
     5401 aagggcaata tgaattggct caacaagttg tatttatcaa tgcaataggc agggattttc
     5461 ctctaaaaat agcgaatgaa acgtatactg atttaaccca attaggcagg aataagttgc
     5521 aggagttgtc tgatttttta attatggaga tgagaagact cgatattagc aaagaggata
     5581 aactgcgaac tcaactgaag ttactcgcag tgatgcgaga gcagtatttt cgcagtaccg
     5641 gcatattctt tgatactaca caaatgattt ctcttttatt gtctttacat catcaagaac
     5701 aaaatgtgtt aatgggagtc agtcgtaatg aaaacctgag cgctccacct gcagcaattc
     5761 ttgctgccat gcaatgggtg cttgctgatg gtggtaccgt cgacctgtgt tttgcaaatc
     5821 acggattaat agctcaggat tatcttgaga agggtgatag agatttcttt acctgtttgg
     5881 gtataccttc ctctgtggta gaggcgaatt ctgagtctgg tacttaccag gttggaggta
     5941 ttaattactc aacactttct gatttgactt cttatcgctc gcacgcagtg atagaggatg
     6001 aggaactgtc tatggataga tgcggctatc ctgtctcaag ctaccttatt cttaatcata
     6061 ttgatttttc taatttagac gatagaactt tctctcattt ttcttcactg tatgattatt
     6121 ataaagggaa ggggcgtgtc gtcaggataa ccgaaataga ggaaaccgtt ggtgaagtgg
     6181 ttgggcaaat cagccggttc gatatggatg tggggtatcg ggttttccct gccaggaaga
     6241 atttgcagga agaattaaat acgcaaagtt tggctgaaag acgaagagaa gaagcagtta
     6301 aacagtattt catacaatct gtttctaata ttcaacaagc ggtattaaat caattggaag
     6361 aatggcaagc atttttgcat ttaatttacc ccaagtcaga atggagacag ctggataatg
     6421 aattatttgc gcaaagaaat gagttgattg attcgctaaa gaagcaatgg acaaagtgtt
     6481 tggaagattc tgatcctaaa aaaatgtatc caaaccccta tgtccgtagg gatgtttttc
     6541 agcgcttaca aaccagagaa ttggaaaagg caattcaagc ctatgaaaaa tctgtttcca
     6601 gcatttggaa cctaaatcga gatcagttga aagcaaaaac agaaaataga ttctccagtg
     6661 actcagtcaa tgcattgcgt tgtaaatatt tggaagaggt cgatttaagt gaacaaataa
     6721 agtggagtaa attatcagct cgtttaaaga aaaaagaagc aactcaggag aaaaagcgca
     6781 gtcatcgtca ccttgagtca gcagtagaag taactggtgc gatgttaagc taccatgatg
     6841 gtgatttaga gccctatcga ttagcttttg tgcacagcca actgaaattg tgggccaggg
     6901 atattagcaa agaaatcaat aactcctcat tacctgccag tgttaaacaa ggcctaatag
     6961 agagagctaa taatgcggat aactttcttg ctttggagtt gttattaatt gattatgaaa
     7021 gcagatggtt agacaaagag aaattgagcg aaaaattccg catgcaacca attatcagtg
     7081 atatgctgag agtgtataag caagcaggtt ttgaagaaca taaagaactt aagattttaa
     7141 aagaaaccta tctggatagt acagctacac aaatcattca tcatcttgac aagacattgg
     7201 cttgggctaa gcctgaaaac agaggacctt ggtattggat tgagcggtca gcagtaaaaa
     7261 aagcggcgaa tgaaatattg gttttggcca acggagtaaa acaagcaaga gattctttat
     7321 ccaaacagat tgctatcaaa aaattatata gagccttatg caaacatcaa gctcaattgg
     7381 aggatgtttg gattttttca tttgatcata aaaatacacg tgatttgatt aatcaaacat
     7441 taaataccat ggacagttta actgttctgg gtagagatga gggtgggctg gatattaatt
     7501 ttattcagga gtgcaaagaa gaagctcaat actatttgtt gggagagaaa ttcagctctg
     7561 ttttagaaga ctttgaaaaa aataacagtc agatcttaac tgctcattct gaatggcaag
     7621 aggttaaaag gcaacttcag caaaagcaaa atgagagtcg caaggtttat acattgaatg
     7681 aaatgtatta tgttctttgt aaaaaaatca atgaattatc catttcatat tccctattgc
     7741 gtgagcctct agtgcaatta cgagggatga ttcgtactct ttggaatagt tttcaggaaa
     7801 aacaccagga tatcatagat gaatcgaaat attttgattt taaagcgcga aaaattaagg
     7861 aggagctgga gggaatagaa ggatattctg tttttaacgt gaaagtaaaa aaaggatata
     7921 caggatttaa tgagtatgtt gagttaatta ttgaaggaga agggacatcc gcattatttg
     7981 aagggtttat gcaatataaa tccaggttaa ctgaattaaa agaggtgtac cgtcagttag
     8041 aattttctct taagcaaaat agattagagt tgctgaatct gaatagaatc catgccaaac
     8101 acttaccttt tttgaaagat cgcatcaagg gcaatcccga agtagagttg tttccagagc
     8161 aatgccgaag caaggtgaag gagattttaa cactaaagga gtggattaat ggtcaaatac
     8221 ctgaaaatct cagtcaattt cctgaaaaca tgcaaaatca ttttcttgac agaaatacgt
     8281 taaaaagcat ggatagaaat acgatgacca tggagcaaat cgataagata aaggatgagc
     8341 agttaaaagc ggatcttaca gaattatata ataaaatgcg taaggtcgat gaagaacagt
     8401 cccaatcatg gttttctacg gtgactcgca ggattgttgg tttgattccc ggcatgttca
     8461 gacaggaaga gacagaggat gattggaggt atcaatttaa tgaattacaa ggacgctcag
     8521 atagcatttt gcaatcgttg ttaactaaaa aaataatgac aaaatatgat gcgttggtta
     8581 atgaaatggt tttaatccaa agcaatctgg cacaacagat ggactctggt aataaaaaaa
     8641 ttcaattttt aagcgataag attcttgaag aagaaaggaa aaccaatgtg gttatcaagc
     8701 gttttgataa cctcaatgat ttttatgatt ttgaggctaa actaaaacgt gttaaaaccg
     8761 cgcctccaat agcctcagta aatgaggttc agatagaatc taaaaacagg atactttctt
     8821 atggtgaggg ggcgttttcc aatcctgaag aggagtttca gacggattct gtaatgcaac
     8881 ctccaatggt ttttaatatt taacgtcagt gatggataag gattataaaa cgagttgata
     8941 tccaaggttc gttcgggctg aggagatgca taagcatcgt ctcgaagcct tggcgcacaa
     9001 cattaaatcc cgtacaaggc ttcgagacgg cataaatgcc tcctcagccc gaacgactcg
     9061 aggtgacaac tgatcttatc cataactcac attaatgaat accaaacgag atatggtgaa
     9121 cctgggcaaa gaactttatt tgtgattctt taaatacaag tcctgtatta ttggcttctc
     9181 tttaatgccg ttcgggttat ttttaatccg aacagcattg ttaaaataca gataatgcag
     9241 gaagtgagga gtagaaaatc ttatttatta attaactcaa taataaaatt aaccacctta
     9301 tccagattga tcagttgagt ttcgcttgaa gttcgggatt tgtactcaat acagccttgt
     9361 tccagatttc tctcactgac cactaatcgg tgaggaatcc ctatcaagtc attatcagca
     9421 aataatacac ctgctctttc attacgatca tcgagtaata catcgatgcc atgagatttg
     9481 agttgctgat aaagtgactc tgcctgttct ttaacggctt gtgatctcgc gccattaaga
     9541 ggaataatat tgacttggaa aggtgctaaa gcttgaggcc agatgattcc gtgttcgtca
     9601 tgatgctgct caatcgcggc agcgactaca cgagtgatcc ctaatccata gcagcccatg
     9661 atcatggttt gtaattgtcc ttgctcattg ataacagacg cattcatggc tttggcgtat
     9721 ttatcaccca attggaaaac gtgtcctacc tctattccac gacagcagtg aagcattcct
     9781 cttccatcag ggcttggatc accttcttta acattacgta aatcataggc atcatcgtat
     9841 tttgcatcgc gctcccatgc agcattgata aagtgtttat ctgcctgatt ggcgccacag
     9901 acaaaggaag gcatggctaa ggcatgatga tcaacaatga cagggatgtt tagctggatt
     9961 ggccctatgg agcctagagg ggtttttaac gtttttaaaa tcagttcttc atcaataaag
    10021 gacaggggag agtgaactaa gggatgtttg gttgctttga cttcattgag ctcatcgtct
    10081 cctcttaaga ccagagccac cataggatgt tccttgcctt tgacaatcaa ggttttgatg
    10141 gtttgattac tggcaatacc aaggaagctg gctacttcat caattgtttt ttgatttgga
    10201 gtatcgacca atgtgattgt ttcattgcat gcttgattcg ctttgggtgg ttttaagctg
    10261 gtcgcttgtt caatatttgc agcatagtcg ctggcgtcac tataaaaaat cagatcttca
    10321 cccgattcag ccaaaacctg aaactcatgg gacgctgaac cgccaatggc tcccgtatca
    10381 gcttcaactg ctcgatattt gagtcccatt ctatcaaaaa tcctgcaata ggcttgatac
    10441 atatccttat aagtttcttg caggctttca atactgaggt ggaaggagta agcatctttc
    10501 ataataaact cacgggctcg cattacccca aaccgtggtc tgatttcatc gcgaaacttg
    10561 gtttgtattt gataaaaatt gactggcaat tgtttatacg attgcaattc attacgcatg
    10621 atatcggtaa tgacctcttc atgggtaggc ccaaagcagt actctctttg attactgtct
    10681 ttcatagtta ataattggcc accaaaggtt tcccaacgtc ccgtctcttg ccataattca
    10741 gcaggttgta ctgatggcat aagcagttcc atggcatgag ttttgttcat ttcttcccga
    10801 actatatttt caactttacg aagcactcta aggccaagtg gcatccaggt ataaagtcct
    10861 gagccaagct tgcgaatcat tcctgatctt agcattaatt gatgggatgc tatttctgca
    10921 tcgttgggag tttctttttg agtgaccaaa aaccattgag atgcgcgcat gtctattcct
    10981 ggatttctat aaataattaa ggtcgatatt atatacttat cacccttcaa tttgcgatat
    11041 gtttagttta ggtccttcgt ttatctgttc ttctctaaaa gactatattt gctcacattg
    11101 tcctataatt attaataaat tggtatttta aattggtaaa aattatgcca aatctacccg
    11161 gattatattt tcttcaagct tatccacgcg aagaaatatg gagattattt gttgatgggc
    11221 gcttctggtc aaaagaaaat ggatggagag gctatgagtc gcgagagcca ggatgcctta
    11281 acgccgctct ggagagttta tgttctattg cattgcaagt tgagaaatca ggagaggaat
    11341 ttgaattaag cgttgattta ataaagagga tacacaagaa gtgtggaaaa aaggtggagg
    11401 aattacagga aaaaaatcca ggtgaactca gaacagatga acctgtttca tttggcattc
    11461 cagctggccg ggcctcgatt aagggtattg aagaattcct gagcctggta tttcttacag
    11521 aaggcgaagc tgaatttggc ccaggcaaag caggtccatt tggcccacgc tttgataaga
    11581 attattttaa aaatctgaat ccagaacaaa taccggatct tgccaagcaa atctatttcg
    11641 atatgtgcaa atatgggcat agtaatacca atcactttta tcttgcagta atgaaaaatg
    11701 tggatgttta cctggaaaaa attacccaat cttacaacca ggaaataaaa accgctgaaa
    11761 ctcttgatga aaaacttaaa attatcgtta agcatattcg aatgtacgaa gtattgcacc
    11821 cttttcgaga tgccaatggc cgtacctttg tcaataattt attaaatatt cttttaatgc
    11881 aacaaggttt accgccagcg acattttacg aacctaatgt ctttgattta tatagcgctg
    11941 aggaattagt tgttgttgtg aaggaggcaa tttttaatac ggtagaaatt attgaacaaa
    12001 gtaaaagaaa gactcccatt actttatatg gatatcattc aagcttggaa gagcaaacca
    12061 aatttcggga catgctggat agcccagcgt atgaaaaaat caaacatatg gatttttcag
    12121 atttaaaccc tgagaaactg catcggaaga ctcaaaaatg tttatcatca ttaaatgagc
    12181 aatatccatt acacagaggc gcaatctatc ttagtgatcc cggtgagata aaattattat
    12241 tgtcaaatcg caatgaatcc cagattaatc agcagataga gcaaggagca ccgcctattt
    12301 atgtcggtaa aactcctgct catttggctg tcatatcagg aaatatggca atgattgatg
    12361 agcttatcgc aaaaaaagcg gatttatctc ttcaggatta tgatggaaaa accgctcttc
    12421 actatgctgc agagtgtgga aatatgcaaa ttatgggaaa aatactaaaa gttgtgctaa
    12481 gccaggaaga tgccatcaaa gtactgaata tcaaggataa tcatggcaaa acagcattcc
    12541 attatgctgc tgagtttgga accccggaat taatatcagc cttgacaacc acagaggtta
    12601 ttcagattaa tgaaccggat aacagcggtt cttcagcaat cactttggca tataaaaatc
    12661 ataaattgaa aattttcgat gaactattaa attccggtgc tgatatcagt gatgagcttt
    12721 tggacgcgat atgggctcgg aaggataaag aaacattagg taaaataatt gccaaaaatg
    12781 agaaaattct tttgaataaa gaagccttca ggatagctat ttctttagga agtgtcagtc
    12841 tggttaaaaa atttttacgc gccggggtcg atattgatat acccttaact aaagataaag
    12901 caacaccgct aatgttatct ataaattccg gtaatcccaa attggtcagt tatttattga
    12961 aaaagggagc taatacacgt ttaaccgata ccagtggtaa ttctgtcctg cattatgttt
    13021 tttattcaaa ggcagaaaac agggaagctt tagcaaatat tataactgag aaagataaga
    13081 aattaattaa tcagccaaat actaatggaa atccacctct ttataatgct gtggtagtta
    13141 atgatttgaa aatggcaaca attttattag aaatgggggc aagggtcgat ttcgaagaca
    13201 ggctgggaaa taatattttg catagtgcca tgagaagatg tgatttgccg attatattgg
    13261 atattgtcaa gaaagatagc accttacttc ataaaagaaa ctctgaaaga agaaatcctt
    13321 ttcatcaggc attgcatgag atgcatacgt ttccatcctc aaaagaaacc gaagaaatac
    13381 acttcatgaa tttaagtgat ttacttttaa aagaaggagt cgatttaaat aaaaaagata
    13441 ttaaaggaaa aacaatatta gatatcgcat tatccaagca gtattttcat ttatgtgtca
    13501 aactgatgaa agcaggtgcc cataccaata tatcttctcc atcaaaattt cttaaaaact
    13561 ccgatgccaa ttctattttg gaacgtcctt ttaaatttaa gaacgattta aaaaaagagc
    13621 tcgatgataa tccgctaatt gctatggcac aaattaatga tttgtacgtc caaatcaaaa
    13681 ataacaggat taggactcct acaggttatg cctcgaaaga aggagtatcc ttttttaaag
    13741 gaaaatcaaa cgatgccaaa gcacatgatg aggttttgtc tgttttaaag gaactctacg
    13801 atagtaaact cacagagatg ctcggtaatc ttcctggtga aggattagaa gaaatcaaaa
    13861 gaagccagaa attttttgac ggcgaactaa aattattaat taaaaaccag gacatttcga
    13921 ggaaagtcga taaaaagtct attcaggaag cagttggtac atccttgaaa ttaaaatggt
    13981 aagaactgct atttcaattc atttttattt tctatttcac catccaagaa ttgcaactcg
    14041 atttttttgt gaaatgcctt gtataattta ttttggtttt ccccaaacaa tgtttggaaa
    14101 accccggttt taggttttat ttcactatct agcatagata atagcaattt aatatcattt
    14161 ttttcgagag aggattcctt agataagtaa gcactcagat catttaatcg ttcctgataa
    14221 ttttttttac tatctaaacc agtcgaaata cgaaaatagt gagttaacac agcctgagtt
    14281 ttatcaaggg tttctttttt aaaagcaggt tcaaaatgag cttcagaaat tgggcactcg
    14341 atttcaggtt gtaccatttc aagtgacaga cgatgaaatg cctcttctaa atcagcctca
    14401 gactttaccc ttgctttaaa tagtggcgcc aggatttgat gtttctcagg gtaattgata
    14461 aaacctcgta tgagctcatc taaatgataa ggaatgcgcg ttacattgat ggttgaacaa
    14521 tcaccgacgg tacgactttt taaatcgtgc attaccatct ccagcgggat ttcaatattt
    14581 tcaaaataag ttattccatc actcaaaccg tcttcaaaga gaattgcttt taatgtctgt
    14641 gctgtttcca catggcctgt cttaataaaa tattccagga cttcattaat ggttttgaca
    14701 gatttttcat ggaagtgttg ttgttctatt tcattaatta attttattaa cgtttttcta
    14761 cgctccaaac tgcgagacat ggcgcgatgg gtaatgatac gagcgatatc aaatgaagaa
    14821 gggtagggag catctgtcaa ttcgtcaaat agcgttttga aatattcttt atccactcct
    14881 atatctcgac ggtcattggt ttgagcaatc aaactggttt tcagctcccc ccaaacccca
    14941 tctgtcattg atattataat atcgccttct gcaagtgtcc gtgtttgcct gacaagcagg
    15001 gcgtctttat ttgcagttgt tgccagggct tgcagggaag gtggagtcca ggtaccaaat
    15061 ccgcgatata tatggcatgc ggataccata tgctttatct taaaacgttt atccaggata
    15121 ataattagtc catcaccaat atttgcaaat tcacctatgt atccttttcc cggatgattt
    15181 tgataaataa aagcagccat cgacgcttct gtatgctgca acgttttcgg tgctgtttgt
    15241 tttttatcag aggcctttgg ttgggtaatc aagcttagag tcgtgtccat attttggtcg
    15301 caattcaaaa aattcgcgca actggaatga gatactctgt atatctcgtc gtcttcttct
    15361 tgatcgccat ttccaccacc cagaccgtca gtaattgcga ttcttgcccc gttaacgagg
    15421 ccatttgtat cagtagtcac taacacacct gatgtatcga gtatggtatc gtattcgata
    15481 tcattctctt ggtgagaaag actggaaccg ataataatgg aatgattatg gctttgattt
    15541 acagagtaaa accgttctga taaaaagctt gataaatttt ttgtttggtc ttttgcaacg
    15601 gattgtgaaa aaataacttt tttcccagtg ataattttat ctctcatatt gcaactcgta
    15661 aaaacaagct tattctcatt aataatatag cgtaacttca tacgccaggc ttaatgactt
    15721 gtagaaaatc ttatacctgg cttgagaaat ttatttcttt ccggtgcttt tacaatttca
    15781 aaaaagttta acttgctttg gcttctaaaa gaatggtttc aaatttttga gccaggagct
    15841 tattttcatc tccatccaaa gtggataact cttcggtata tgttttacag ctcacttttc
    15901 cactgcttac atcataaata gcaccaacca tccctatctc attgctgtca atcatagtcc
    15961 gtaaaatatc acttttttta taaatgttct gtaaggtatt cgcaacattt aattcagtga
    16021 catgatttac aaaaatttga ttttttccgt tacgttctgt tgtagtttct ttttctgcat
    16081 taactgcagg cttaatttta gagagcaatt gggtgatatg acccttttca ataccatcac
    16141 aggcagattg aatggcgccg catcgggtat gtcccagaac catgataagc ttcgcaccaa
    16201 ctacgttaca ggcgtattct atgctggcca aaatatcgtc attaatcaca ttgcctgcta
    16261 tgcggacaca aaaaagatca ccgaaactca tatcaaagat ggtttctaca ggaactcttg
    16321 aatcgataca ggccaaaacc acagcaattg gatgttgtgt ttttgcagtg tattttatat
    16381 cgatttggtt tgatcgatga atccggttat cgcttaaaaa gcgctgattt ccctcaagca
    16441 gtatgtttaa tacttgtgcg ggcgtcaggt tagtttgaac atcataggtt gtaaccgtaa
    16501 tgaaatcgat gtaattatga attttatagt gttcctgaaa tccggtgaaa ttcaaagaaa
    16561 tttgcttact gcctgctaac tcttctttca actcattaaa taactccaga atttctttat
    16621 caatgtattg agaataacgt gcgtctataa tcagttgaga atgtctgggt aatgtatcca
    16681 attcagcaac cagtgcggct ttgttaagaa aagttatttg ttggggaagc accagacggt
    16741 atgttgaacc atttggataa atttccttga tgatatcaat acgagcctga ctattggact
    16801 ttaatatata aaagaggctg atggccaaac cgattaatat acctgcaaga agattgaaag
    16861 caataatact gattacggtc actataaagg gaataaatcg atcactacct tgtgaaaaga
    16921 tattaatata gatggctggt ttgtttaatt tataaccagt ataaattaaa atcgcagcaa
    16981 gagaagacaa aggaatttta ttcaatgcgc ctggtatcaa catgacagca aacaagataa
    17041 agaaaccatg aagtatggct gaaaatttgg tttttgaacc ggcgtggata ttgatggagg
    17101 ttctgacaat aactgatgta acaggaatgc ctcctaccaa acctgctgtt aaattaccta
    17161 aaccttgagc aaccaattcc tgattagttg gactatgtct tcttttttta tccaatcttt
    17221 ctgatgcttt gagattgagc agtgtttcca aagaagcaac aatacatatg actagcgcat
    17281 acaggtagac tttgggatta gtccaggcgg accagtcagg gtactcaaga tggctgaaaa
    17341 attggaaaaa accattggta tctgggatat taaccagttg aggcgagttt tgtgccaggc
    17401 tagagtttgc ccagatgaac agttcattta acagtattcc tgccagaact acaaggatag
    17461 gggctgggat ttccttcaga attttattct tggttatatc aaaatagatt aaaatagcca
    17521 atgaaagggt agtaatgatg agtgcgccct cattaatatg ctgcgacaaa gccaacaaag
    17581 ggcttactgt aaatccttcg gttgtttcca ataaatgcgt tttaagttca tcaaagtctg
    17641 atgagagggt gaatgcgagt ggcaattgct taatgattag caagatcccg attgaacaca
    17701 gcaagccttg tactacgttg gaaggaacgt aatctgcaac aaatcctgct cttaacgcac
    17761 ctattatcag ttgtaaaaca cctgcaatag taagagccaa taaaaaggta ttaaaatcac
    17821 caagttgcga gattgctgcc aatacgacgg ctgccatacc cgcagcaggt ccgcttacac
    17881 ttacctgtga gccgctaaat atacccacaa caataccgcc aatgattcca cttaatatac
    17941 cagaaaaaag aggtgcgcca gaagccaggg caatacctaa gcataaagga atcgccacga
    18001 ggaaaacgac aattgcggca acaaaatcaa attttaaata acgtttagag tagatacgaa
    18061 ata
//
LOCUS       DATNSN010000053        17940 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.053, whole genome shotgun sequence.
ACCESSION   DATNSN010000053 DATNSN010000000
VERSION     DATNSN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17940
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.053"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(22..660)
                     /locus_tag="VAH76_001479"
     CDS             complement(22..660)
                     /locus_tag="VAH76_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO1386111.1"
                     /translation="MTQFEKTFNFMKKIWVIITYIILVVLSYYFLDRTLATYFHSLDL
                     RVNAHALTYLTALGKWKIYVALFLITALYFRYIQQNKQYEIRSWYLLGCVLLPNLLTF
                     VLKISFSRARPDLLFDNNLYGFYWFQLNDNYWSFPSGHSITITALAAGLGFLFPRYFF
                     LFIGAALLVAATRVILYHHYLSDVMTGFYISMLLVGLFTQYLQRNHYLNKIN"
     gene            917..1576
                     /locus_tag="VAH76_001480"
     CDS             917..1576
                     /locus_tag="VAH76_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEO1386112.1"
                     /translation="MPEFTLIQQICIWALPVLLAITFHELAHGYVAYRCGDNTAKMFG
                     RLTLNPIKHIDPIGTVLLPLLIGVITNFNFVIGYAKPVPVNQSQLRKPRRDMILVTLA
                     GPLANLIMAFLWAGCFKIATFLNPQSSMAALFLLVTARAGMMINLILAFLNLIPIPPL
                     DGSKVVINLLPARQAIAYAKLEPFGFLILIVLIFTNVLGYILTPLIYGSLYILSAIFN
                     L"
     gene            1573..2625
                     /locus_tag="VAH76_001481"
     CDS             1573..2625
                     /locus_tag="VAH76_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HEO1386113.1"
                     /translation="MNILADALLPGLDSAFPPPFTVTLYHKADEIPELLHHKDVLLCR
                     STLKINGDLLKNHQIKVVATATSGTDHIDFPFLESQNISIIDAKGCNAISVADYVVAC
                     LAYLDKQQLIQGKTAGIIGLGQVGTKVYERLNAAEFQLCLYDPPKATRDTSFQSCSLE
                     DLFECDLLCVHAELHSDAPYPSLNLINRDFLKELKPGCIIINASRGGIVNEEALLHLG
                     SAILYCTDVYNNEPHIDSRIVSKATLCTPHIAGHSLEAKFAAVAIVSRKLHQMLGLPY
                     PQFATPEKPYRLNENSDWRELALSIYNPIHETLELKHAGNLSSAFLTLRKNHHHRHDF
                     TTYFDSDSIKKYPLLG"
     gene            complement(2670..4121)
                     /locus_tag="VAH76_001482"
     CDS             complement(2670..4121)
                     /locus_tag="VAH76_001482"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEO1386114.1"
                     /translation="MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAAS
                     DGRLYIEKAVASGAVAVVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPS
                     QFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
                     LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYG
                     AAKSRLFERAELQWAIINQDDDFQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDIT
                     GTEIEVKSPWGHQLLKIKALGKFNIYNSLAVYSSLLASGHNPEKVVQVMAELKAAPGR
                     MEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAA
                     SMYADNIVITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDI
                     ILIAGKGHESYQQIGNTKHVFSDQEVVRRLMNN"
     gene            complement(4143..5804)
                     /locus_tag="VAH76_001483"
     CDS             complement(4143..5804)
                     /locus_tag="VAH76_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO1386115.1"
                     /translation="MKKTHHFARLVTVAVFFSLILAVLIWRMVDLTVLHRQFLQGQGD
                     ARSIRVIDIPAYRGMITDRNGTPLAVSTPVESVWVNPKEFSPDEEQFMQLAKYLNLAP
                     KELSRKIVRAENREFVYLQRQLPPPLSKKIEALKIPGVNFQKEFKRYYPDSDSISPLI
                     GFTNVDDQGLEGIELAYQDWLMGVVGKKRVIKDRLGRVVEDLGVLKEPRPGHELTLSI
                     DRRLQYLAYSELGKTVEEFSAKSGSVVVIDAENGEILAVANVPSYNPNARGHYDKETY
                     RNRAFTDTFEPGSVIKSFSIASALETGLFTPDTIIDTNPGWMTVHGRTVRDIHNYGVL
                     DVTGILQHSSNVGVTKMVLSTPAEQLIGFLQKCGFGQRSELAYPGESEGGIVKAKDAN
                     PFVLATLGFGYGLSVTALQLAKANLIFANKGRLIPVTLIHNDTPSNGIQVMQPKTAEN
                     VLLMMEAVMNKGTGKGARVPGYRVAGKTGTARVAGKNGYKDRKYTASFIGIAPVSKPK
                     FVVAVIIHEPSRKGYYAASVAGPLFAKVMSGALRLFNIPTDEPVS"
     gene            complement(6186..6524)
                     /gene="ftsL"
                     /locus_tag="VAH76_001484"
     CDS             complement(6186..6524)
                     /gene="ftsL"
                     /locus_tag="VAH76_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO1386116.1"
                     /translation="MNAAAKVINQGNLFNGQLADMQMSKSLYMLIVLLAAVLVSAFAV
                     IYSTNSYRLTLSQVQQEEQQTHFLQLQWGQLLLEQASLATPARVEELAREKLGMTLPT
                     SANTYLLHPQ"
     gene            complement(6521..7447)
                     /gene="rsmH"
                     /locus_tag="VAH76_001485"
     CDS             complement(6521..7447)
                     /gene="rsmH"
                     /locus_tag="VAH76_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO1386117.1"
                     /translation="MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSD
                     KGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLG
                     VSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
                     GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDL
                     SNCLEECLDVLGPRGRLAVISFHSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNF
                     KRVGKAVKPQSSEIKENVRSRSAVLRIGEKLA"
     gene            complement(7459..7917)
                     /gene="mraZ"
                     /locus_tag="VAH76_001486"
     CDS             complement(7459..7917)
                     /gene="mraZ"
                     /locus_tag="VAH76_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEO1386118.1"
                     /translation="MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETC
                     LLLYTAAQWQIIEDNLQKLPSFNAAARRIQRLLIGHATDVEVDANGRVLLPTVLRNYA
                     KLEKDVVMIGQGNKFEVWNKELWESKREQWLAEEASMTDGLPEEMKTFSL"
     gene            complement(8530..9300)
                     /locus_tag="VAH76_001487"
     CDS             complement(8530..9300)
                     /locus_tag="VAH76_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEO1386119.1"
                     /translation="MILCIDVGNSHIYGGVFDGDEIKLRFRHTSKVSTSDELGIFLKS
                     VLRENNCSPETIRKIAICSVVPQVDYSLRSACVKYFSIDPFLLQAGVKTGLNIKYRNP
                     VEVGADRIANAIAATHSFPNQNIIVIDFGTATTFCAISHKKAYLGGAILPGLRLSADA
                     LSKNTAKLPSVEIIKTESVVGRSTIESIQSGVYYGVLGACKELIQRIHHEAFNGDKIL
                     ILATGGFASLFDKQGLYDHLVPDLVLQGIRLAAMMNTA"
     gene            complement(9569..10126)
                     /locus_tag="VAH76_001488"
     CDS             complement(9569..10126)
                     /locus_tag="VAH76_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1386120.1"
                     /translation="MKKQILLVPVCVLAASCASMDRSTAIVDDAGYTHYTMSMESDRK
                     GSNYFPEKRQATGKKVFIFDPKATAWAAYDAQGNRVKTGSASGGKDFCEDVGRPCRTV
                     TGSFKVYSKKGEECTSSIYPIETGGGAKMPYCMHFHGGYSIHAAYEVPNYNASHGCIR
                     VLPSAAKWLNQNFIDVGTSVIVKPY"
     gene            complement(10203..10610)
                     /locus_tag="VAH76_001489"
     CDS             complement(10203..10610)
                     /locus_tag="VAH76_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEO1386121.1"
                     /translation="MRPCYFVCILALFMFAMPGYSENHHPQEFLKSIAGTKDEGEKIF
                     NHFCVNCHGTKPMISLGAPEIGNERAWGARLKQGMEVLFKHTDEGFNAMPPRGGCFEC
                     TDKQLILAIIAMVPKQAQKGLLNNLLDHKEYKQ"
     gene            10697..11398
                     /gene="flgA"
                     /locus_tag="VAH76_001490"
     CDS             10697..11398
                     /gene="flgA"
                     /locus_tag="VAH76_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEO1386122.1"
                     /translation="MKKCILSFFLFFASTSLFSESSQSLELLKNKIEQYALNELSNYT
                     EGKIQVTADNIDPRLNLKVCDDNKLEVFNPYQTPMLNTSTMGIKCLEENNHWTLYIPV
                     KITIFKSVLVAKRALLKGTKISNSDIYQTELDVQKLKQGYFTDSKELIGLVCKHDITP
                     NSPLNPFNIELAKLVHKGEQVSIIAAHDNLTVSMDGIAMDEGALGDSVKVKNLSSKRI
                     IEAQVTGKKTVKVIF"
     gene            11489..11809
                     /gene="flgM"
                     /locus_tag="VAH76_001491"
     CDS             11489..11809
                     /gene="flgM"
                     /locus_tag="VAH76_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEO1386123.1"
                     /translation="MINTIEDIHMVNQINDSANLRHIDMDNRINANHKEVKSPVLENN
                     STDSVNLSSTSKQLEALKASLKDLPEINEARVLYFKAEIQSGQYEIDSSKIAHGMLNS
                     VEMV"
     gene            11826..12323
                     /locus_tag="VAH76_001492"
     CDS             11826..12323
                     /locus_tag="VAH76_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEO1386124.1"
                     /translation="MNNNNKAKTLITQLEQEISWVEELNTLLSEEKEILSTSQFNQLE
                     TLAEKKQELSTKLEASAKQRVDLIKESNPGDKPHGLLLSDFLKNCATEDGIQINHLNN
                     TLAEKLNLCRELNTINGQVIANNMYTRQQIVNALSGNKSNAVSVYTSNGNLETPSESS
                     HHEEA"
     gene            complement(12386..13129)
                     /locus_tag="VAH76_001493"
     CDS             complement(12386..13129)
                     /locus_tag="VAH76_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1386125.1"
                     /translation="MNFDNQIALVTGGVSGMGKACVQYLQQHGMKVVVWDKQEGTSNE
                     AELYVACDVTSDESVEKAMRQTISQVGVPRVCINCAGIAPAKRMVGKEGPMPLESFRQ
                     VIDVNLIGTFNVMRIAAHAMSGLELDNKSQERGVIINTASIAAFEGQIGQSAYSASKG
                     GIVSMTLPAARELAQFAIRVNTIAPGLIATPLLLNMPQEVQDSLVATVTFPKRLGRPE
                     EFASLVGHIIENQMINGEVIRLDAALRMR"
     gene            complement(13338..13886)
                     /locus_tag="VAH76_001494"
     CDS             complement(13338..13886)
                     /locus_tag="VAH76_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO1386126.1"
                     /translation="MLLLNRADSLLVLIDVQEKLTPAILNLDLFLARCEWLLKLARKL
                     DVPVLASEQYPKGLGRTVSPLSSYINQEECIEKIHFSCMQEPQYINLLKGYNKNQLIL
                     IGIEAHVCVLQTAIEMKGYGFEVFVVVDGVSSRSEFDLKYGLKRMKQNGIHLITAEMV
                     LFEWLKQAGTPEFKAISKEFLQ"
     gene            complement(14046..15137)
                     /locus_tag="VAH76_001495"
     CDS             complement(14046..15137)
                     /locus_tag="VAH76_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO1386127.1"
                     /translation="MEMMRQQRNLLLIPFILCFILARPVFSLNLDALLPDERNTVEVF
                     QKASSKVVYVHRLANATVQRRYSLQKTHVPDGAGSGIIWDNKGHVVTNFHVINGADDI
                     AITLGNMTVPAKVIGSEPRKDIAVLEIKSPKALDYLKSFQPFEIVSLNDLIVGQKAIA
                     IGNPFGLDHSLSKGVISALGRKVPGIGGVTIYDMIQTDTPINPGNSGGPLLNSAGQLI
                     GMNTMIYSRSGSSAGIGFAVPAEDIQKIASQIINHGRVVLSGIGIQRVEPHLAQRLGV
                     KKGILIADVVPGTPADKLKLRGTHRDQWGRIVLGDVIVGVNAHPVPNYDALYNLLTEI
                     KVGEQITVSILRNGKKMDVKMKTIDIAAL"
     gene            complement(15112..15450)
                     /locus_tag="VAH76_001496"
     CDS             complement(15112..15450)
                     /locus_tag="VAH76_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386128.1"
                     /translation="MVYIKRDKDGQICAVYSQKTSGVTEQIDINDAEFVDFLLRCESD
                     KKLKFLQSDLQLIRVLEDLIDVLIDKNIITITDFPQSVIDKLLARQAFRKRFASRAGM
                     ELNGDDETTT"
     gene            complement(15669..16565)
                     /locus_tag="VAH76_001497"
     CDS             complement(15669..16565)
                     /locus_tag="VAH76_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386129.1"
                     /translation="MEPVTIALICAASFGAVVALSAFIRQLLLSRDKNLNDEAQRKAI
                     NQEAGELEKMREQMQSNKRFDSHYKVLGANKDAIIYLDNKIEDVLRKKAQLVERYAQV
                     SLKESGAIVEGGISIERKDACDRLKKEIDEEIKFYDSELLHLQQRRTSLWDTHTDLQE
                     YLLGQEKSRNESLDHLYKQHTSLLEKIYIRHTDDSENIAKQSIDAGTTSFKAIILAPL
                     QFLLQYFNISSGINFDQSKLENAARDDVDKTESDINDSENSDDEIYSDTDVNSDDESN
                     RSDNEDSQDESDSENDSRLMFA"
     gene            complement(16576..17232)
                     /locus_tag="VAH76_001498"
     CDS             complement(16576..17232)
                     /locus_tag="VAH76_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386130.1"
                     /translation="MHQKQSEIVVLKPTNLFLSFLASQLPEANLPSLKLLHTDNTAYV
                     IPKYDSDEDTLNEIEKHFTTMFRHEICRWLGRSAHNEIETSFLDFLCCFKFQLHSHII
                     LMEPSLKEGRQMLNVKPRSALLEWMKCTVEDQEELSDVMSRVNLAQIAENSTVIVKNF
                     TAIKDVKPFIKQYFKPIFETTMSRVSGQPTQWPQVNSFQSFSRYFAVEIHTQLIHLHS
                     "
     gene            17652..>17940
                     /locus_tag="VAH76_001499"
     CDS             17652..>17940
                     /locus_tag="VAH76_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEO1386131.1"
                     /translation="MKLLGKILLAFTALMFFVSITLWILAKNIKPETIKQLVSNKITA
                     ITHKQSHIDGTISWQLFPRPGLKFTKIHIGDDNLNDNYSLLIDTLLLNLKIT"
BASE COUNT         5594 a         3765 c         3007 g         5574 t
ORIGIN      
        1 tttgactacg atttatagag tctaatttat cttattgaga tagtggttcc tttgcaaata
       61 ctgtgtaaat aagccaacaa gcaacatact gatataaaat ccagtcataa catcacttaa
      121 ataatgatga tataaaataa ccctggtagc agcgacaagc aaggctgctc cgataaagag
      181 gaaaaaatat cttggaaaga gaaaccccaa tcccgcagcc aaagcagtta tagtaataga
      241 atgccctgaa gggaatgacc aataattgtc attgagttga aaccaataaa acccatagag
      301 gttattatca aacaagagat ccggccgcgc gcgactaaag ctaattttta acacaaaagt
      361 caataaatta ggcagcaaaa cacagcccaa taaataccag gatctaattt catattgttt
      421 attttgctga atgtatcgaa agtataatgc ggtgattaaa aacagtgcaa cataaatctt
      481 ccattttccc aaagccgtca agtatgttaa tgcatgagca ttgactcgaa gatccaggct
      541 atggaaataa gtagccaacg ttctatccaa aaaataataa gagagaacaa caaggataat
      601 ataggttatt attacccaga tttttttcat gaagttaaaa gttttctcaa attgcgtcat
      661 cgaaaccaag ccttataaat cctcatatca aatggaatca tataagatgc tttcgattta
      721 gccaagaccc catgttatta ataaaaatta ggtcctgtcg agatttctca cgcttgagta
      781 gaaaatcaag atttttcggc aaggatggta aaaatatcaa caaatgcgag cggccacaca
      841 atttttcatc aacccttgtt attagcgagt agaattcgtt aaaatcagga tccttattaa
      901 atacatcaga atattaatgc cagaattcac gttaatccaa caaatttgta tttgggcttt
      961 accagtatta ttagctatta cttttcatga gttagctcat ggttacgttg cctaccgctg
     1021 cggtgacaac acagctaaaa tgttcggtcg attaacatta aaccctatca aacacattga
     1081 tccaataggc actgttctat tacctctttt aattggcgtg ataacaaatt tcaatttcgt
     1141 aattggttac gcaaaaccgg ttccggttaa ccagagccaa ctgcgaaaac cacgccgaga
     1201 tatgatattg gtaacgcttg cgggaccatt agcaaatctg ataatggcat tcctatgggc
     1261 tggatgtttt aaaattgcaa ctttcttaaa tcctcaaagc tcaatggctg ctttattcct
     1321 gcttgttaca gccagagcgg gaatgatgat caatttgatt cttgcctttt taaacttaat
     1381 ccccattccg ccacttgatg gcagcaaagt tgtaataaat ctattgccag caagacaagc
     1441 tatcgcttat gcaaaattag aaccatttgg ttttttaata ttgattgtat taatttttac
     1501 taatgtattg ggatatattc ttactcccct catatatggt tcattatata ttttaagcgc
     1561 gatttttaat ttatgaatat tttagcagat gctttattgc caggattgga tagtgctttt
     1621 ccaccaccat ttaccgtaac cttgtatcat aaggccgatg aaattccaga attgcttcat
     1681 cataaggacg ttcttctttg tcgttcaact ctaaaaatca atggcgattt actaaaaaac
     1741 catcaaataa aggttgttgc tacagcaacc agtggaaccg atcatattga ttttcctttt
     1801 ttagaatcac aaaacatatc tattattgat gccaaaggat gcaatgccat atcggttgct
     1861 gactatgttg ttgcttgcct tgcctattta gataaacagc aacttattca aggtaaaaca
     1921 gcaggaatta ttggtttagg gcaagtaggt actaaagttt atgaacgact aaatgctgcc
     1981 gaatttcagt tatgccttta tgacccaccc aaagcaacca gagatacttc ttttcaaagc
     2041 tgctctcttg aagatctttt tgaatgtgac ctcttatgtg ttcatgctga attacatagc
     2101 gatgcgccct acccaagcct taacttaatt aatcgggatt ttctcaagga attaaaacct
     2161 ggctgcatta tcatcaatgc ttctcgagga ggtatcgtta atgaagaagc ccttttgcat
     2221 cttggctctg ccatacttta ttgcaccgac gtctacaata acgaacctca tatagatagc
     2281 cggattgtct ccaaggcaac tctttgcaca ccacatattg caggacacag cctcgaagct
     2341 aaatttgctg cagtcgcaat cgtcagtcgt aaattacatc aaatgcttgg tcttccatac
     2401 ccacaatttg caacccctga aaaaccttat aggttaaatg aaaacagcga ctggagagag
     2461 ttggctttat caatatataa cccgattcat gaaacactag aattaaaaca cgctggaaat
     2521 ctatcctcag cattcttgac tcttcgcaag aatcatcatc accgccatga tttcacaact
     2581 tattttgatt ctgactcaat aaaaaagtat ccattattag gttaaagtgt tgatgtctgt
     2641 ttctaataat taacccctgt cccgaaattt taattattca tcaaacgtct tacaacttcc
     2701 tgatcagaaa aaacatgttt tgtattccca atctgctgat acgattcatg tccttttcct
     2761 gctattaata tgatatcgtt atgctcagcc tttcttaaag cataagcaat tgcctcttct
     2821 ctattcacca gtttagagac aggcgcagag gcaggaatgc cacaagcaat ttcatttaga
     2881 ataatttccg gatcttcact tctcggatta tcgctggtaa tgacaatatt atcagcgtac
     2941 atgctggctg cctttcccat aatgggccgt ttggttttat ctctgtcccc accacaacca
     3001 aatacaaccc acaatctccc tttttttatt tgttttaagg tgataagaac attttccaat
     3061 gcatcgggag tatgagcata atcaacaaga acatatgggg attgagcgac aatttccatt
     3121 cggccaggag ccgcttttaa ttcggccata acttgcacta ctttttctgg attatgcccg
     3181 ctggcaagca aactgctata aacagccagg ctattataaa tattaaactt acccagtgct
     3241 ttaatcttta gaagctggtg cccccaagga gatttgactt caatttctgt tccggtgata
     3301 tccatattcc attggagcgc tttcacatcg caattttgat gcataccgta agtcactttt
     3361 tttacatgag tctttagcac attgctcatg agatcttgaa agtcgtcatc ctgattaata
     3421 atagcccatt gcaactctgc tcgttcaaac aacctcgact tcgcagcccc ataagcttgc
     3481 atagtttggt gataatctaa atgatcgagc gttaaattgg taaagatagc ctgactaaat
     3541 tcgagagagt cgactctatg ctggctcaac gcgtgagaag atacctccat acaaacctgt
     3601 ttaatgccct cttctttgta ctggtgcatc aacctctgca aacaaagagc atctggcgta
     3661 gtattttcca aaggttttaa agcatccacc ttgccttgtc ctatagtccc gatgtaagcc
     3721 gagtgctgcc cgagtaaatc atgtgcctgt gccaactgat aagcaatagt cgtcttgcca
     3781 tttgtgccag ttaccccagt taaaccaaga aattgggatg ggttattaaa aaaccgtttc
     3841 gctatttccc ctaatttttc agctaattgg ggaatggcta tacagggaat tgctgttggc
     3901 aaagtaattc cctgaggtaa attattgggc tcataaacca cagcgacagc acctgatgcc
     3961 acggcctttt ctatatataa tcgaccatcg gaagctgccc ctggataggc gacaaataaa
     4021 tccccttctt tcaccaagcg gctgtcattt tttatgtcac tcacagtgcc atcaataatg
     4081 tcccttttaa tccacggttc taataattgg gttaatttca caaaattggc tcctattctt
     4141 ctttaactaa ctggctcatc agtaggaata ttgaataagc gtaaagcacc tgacataact
     4201 ttagcaaata agggccctgc cacagaggca gcataataac cctttcgtga aggttcatga
     4261 ataataacag cgactacaaa ctttggtttg gaaactggtg ctatcccaat aaaactggct
     4321 gtgtatttcc tgtctttata tccattcttt ccagcaactc gtgctgttcc agtttttcca
     4381 gctaccctat aaccaggaac ccttgctccc ttacctgtac ccttattcat taccgcttcc
     4441 atcattaata atacattttc agcggtttta ggttgcatca cttgaatgcc attactgggg
     4501 gtatcattgt gaatcaaagt cacaggtatt aatcgtcctt tatttgcaaa aattaaattg
     4561 gctttagcca attgcagtgc agtaacagac aagccataac cgaaacctaa tgtggctaaa
     4621 acgaatgggt ttgcatcctt ggcttttact atgcctcctt cactttcacc cgggtatgcc
     4681 aattcagagc gttgcccaaa accacatttt tgcaaaaacc cgattaattg ttcagctgga
     4741 gtggataata ccatttttgt aacacccaca ttactggaat gttgtaatat tccggtaaca
     4801 tctaaaacac cataattatg gatatctctt actgttcggc catggacagt catccatcct
     4861 ggattagtat caataatggt atccggagta aataagcctg tttccaaagc actggctata
     4921 ctgaatgatt taatgacaga cccaggctcg aaagtatcgg tgaaggctct gttgcggtag
     4981 gtttccttat cataatgccc acgtgcgtta ggattataag atggaacatt agccacagcc
     5041 aggatctcac cattttcagc atcgattaca actacagacc cagatttagc agaaaactct
     5101 tcaactgttt tacctaattc gctataagct aaatattgta gtcgtctatc tatactcaaa
     5161 gttaactcat gtcccgggcg tggttccttc aaaactccca gatcttcgac aactcgtcct
     5221 aatcgatctt tgattactct ttttttacca acgactccca taagccaatc ttgataggcc
     5281 agctcaatcc cctccaatcc ttgatcatcg acattggtaa atccaattaa tggggaaata
     5341 ctatctgaat ctggataata acgtttgaat tctttttgaa aattaacacc tgggattttt
     5401 aaagcttcga tctttttcga aaggggtgga ggtaactgtc tttgtaaata cacaaactcc
     5461 cgattttcgg cccggacaat ttttctactt aattctttag gtgccaaatt caggtattta
     5521 gctaactgca taaattgttc ttcatccgga gaaaattcct ttggattgac ccaaaccgat
     5581 tcaacaggtg tgctcacagc aagcggagta ccatttctat cggtgatcat acctcgatag
     5641 gcaggaatat ctataactcg gatgctgcgc gcatcaccct ggccctgcaa aaattgccta
     5701 tgcagaacag tgagatcaac cattctccat atcaaaacag ccaaaatcaa agagaaaaaa
     5761 acagcgacag taaccagcct ggcaaaatgg tgtgtttttt tcatatatga ctactcataa
     5821 atagtattag ctatgcacgc aacaataaat tagtgaaaat atacttgaaa cctctttctc
     5881 aaaattacct tagagttaaa cataaggcac gtatacctgc ctggaattta tcaggctatt
     5941 ttacacttag tcttccatga cgtcctggaa agaagatgaa tgacgtgtaa gtgtacatga
     6001 agctttcaat tttttcgagg tgatttcctc cgcattgcgc ctaattgctc tatcaaatcc
     6061 tttgaaattt cattattcaa tctttaattt gatgctcttg gacatagatt atccttcacc
     6121 aggtactcag ctcttaccta taactcaaaa tatggaaaca ctttaactcc tctaaaacga
     6181 ctcacttatt gaggatgcag taagtacgta tttgcagaag ttggtaaagt cattcccaat
     6241 ttttctctgg ctaattcctc aacccgggct ggagttgcca aactggcctg ttccaataat
     6301 aattgtcccc attgcaattg taaaaagtgg gtttgctgtt cctcttgttg cacttgactg
     6361 agagttaatc tgtaggaatt agtgctatat atgacagcaa atgcactgac tagtacggca
     6421 gctaacaata ctatcaacat atagagtgat tttgacattt gcatatctgc caattgacca
     6481 ttaaataaat taccttgatt aatgactttc gctgcagcgt tcatgctaat ttctccccta
     6541 tcctaagtac tgcacttcga gatctaacat tttcctttat ttcgctactc tgtggtttca
     6601 ctgctttacc tactctttta aaattggtct ttagttcttc gtatttgatt gggacttcaa
     6661 caggcggcct gttaccttgc tctttatctc tcataaattg tttgactatt cggtcttcca
     6721 aagagtggaa actgataacg gccaagcgtc ctcttggccc caatacgtcc agacattcct
     6781 ctagacaatt actcaaatcg gttaattcct gattgacatg tattcgaatc gcttgaaata
     6841 cccgggttgc aggatgtttg tgtttttccc atttggggtt tgcttcttta acaatttctg
     6901 ctaattccaa agtagttgtt attggtttta gttttcttgc atcaacaata gcttttgcaa
     6961 tcctgcctgc aaatcgttcc tcaccataag ccctgaatac atgagccagc tcgttgactt
     7021 ctgcttcatt gacaaaattc gcagcattaa ttgattgagt taaatccatt cgcatatcaa
     7081 gagggccttg cagcataaaa ctaaaacctc tttctggatt gtccaactga ggtgaagaca
     7141 ctcccagatc aagcaaaata ccatcaacgg ccccaattac tcccgcttgt gacgcaaatt
     7201 ccttgatttg cgcgaatgaa ccgtgaaaaa tttgaaatcg tttatccaaa ccaaaataat
     7261 ccttggcata ttgcaccgca tcaaggtcct tatctattgc aaacagtctt cctttatcgc
     7321 ttaaatgatt taaaatctcg cggctgtggc ctcctcggcc aaaagttcca tcaaaataga
     7381 tgccgtcagc ttttatagcc aaacctttga ttgactcatg taataaaaca gattgatgct
     7441 ttgccatttc tctttccgtt ataaagaaaa cgttttcatt tcctcgggta acccatccgt
     7501 catggacgct tcttctgcta gccattgctc acgcttggat tcccataatt ctttattcca
     7561 aacctcaaat ttatttccct ggcctatcat cacgacatct ttttctaact ttgcataatt
     7621 tcttaaaact gtgggtaaaa gtactcgtcc attcgcatca acctccacat cagtggcgtg
     7681 cccgattaac agacgttgaa ttcgtcttgc tgcagcatta aaacttggta gcttttgcaa
     7741 attgtcttcg ataatttgcc attgtgctgc tgtgtaaagg agtaagcagg tttcttcggt
     7801 atcaatagtc accacgagag ggattttatc ctctgcaccc aaggcacttc tgtaacgcgt
     7861 cggaatagct aagcgccctt tagtgtctat ggtgatggca ttaattccac gaaacatatt
     7921 atttcccaag tgaaggagaa ttattctccc acaattctcc acttttttat tattttaacc
     7981 cacttttcta cactatagga acacatttct acacacgtca agctgaaata ccacttttgg
     8041 agtaattttt tttatggatt aaagaggcaa atcccaaact tgttatttat ttgatcttta
     8101 agaaagattt gtaaccaaat gtatgctcac agttataagt tctctcgaat catctgatta
     8161 ttttgcttca aggaaagagc agattttttg aggagcaggg tttgccagag taaatgagca
     8221 ccacaagaac atttccaacg cagcaataaa atgaaagaga cagattatgc aagtgagcgg
     8281 tctagaattt gtttggctca taggttgatc ttgaattgat taatgaaaca gaaggaagag
     8341 cccagagttt ttctaataga aaaagcggga ggtaaaacgc taaaaatgga aatcctgagc
     8401 aaaagggttt cctgctttat aagactcgaa atcctctctt ttgattaact taccagaata
     8461 caaaacaccc cttaaactcc attagaataa acagaaataa atctgatatt aacaaaaacc
     8521 ttttactgct caggcagtat tcatcatagc agctaatctt atcccctgga gaaccaaatc
     8581 tggcacaaga tggtcataaa gaccttgctt atcaaataaa gacgcaaatc ctcctgttgc
     8641 caaaattagt attttatcgc cattgaacgc ttcatgatgt attctttgaa ttaattcttt
     8701 acatgcacca aggacaccat agtagactcc agactgaatg ctttctatgg tagaacgccc
     8761 aacaacactc tcggtcttga taatttcaac agaaggtagc tttgcagtat tttttgacaa
     8821 agcatcagca gataatctaa gaccaggtaa aatagctcca cctaaataag cttttttatg
     8881 agaaatggca caaaaagtag ttgctgtacc aaaatcaata acaataatgt tttgattggg
     8941 aaaactatga gtggcggcaa tagcatttgc aattcggtca gctccaacct caaccggatt
     9001 tctatatttg atattcaatc cagtcttaac tcctgcttgt aataaaaacg ggtcaataga
     9061 aaaatacttc acacaggctg accgtaatga gtaatctact tgaggtacta ctgaacaaat
     9121 agcaattttt ctaattgtct cgggagagca attgttttca cgtaaaacac ttttaagaaa
     9181 gatgcccaat tcatccgaag tacttacctt ggaagtatgg cgaaaccgga gtttaatctc
     9241 atctccatca aatactccac catatatatg agagtttcct acatcaatac acagaatcat
     9301 tattaaatct actttttaaa tggcataact agtgatttgt gattataggt ctaagataat
     9361 aattcagcaa tcccggaaag aaatagccct gtattgagag cattctatat attattgatt
     9421 caaattattg ttagatccgc tttgctccca acccaaactt tagctggact tcttacgaaa
     9481 ccattatcct cttgtctgca cgagataata aaatcatcca ataaaaaaac cccgcaatta
     9541 gcggggagta gtggagaatc tttacttatt aataaggttt aacgattacc gaagttccta
     9601 cgtcaataaa gttttgattt aaccattttg cagcgcttgg taaaacccgg atacagccgt
     9661 gacttgcgtt atagttggga acttcatagg cagcatgtat tgaataacca ccatgaaaat
     9721 gcatgcaata aggcattttt gctccaccgc cagtttctat tggatagata ctggaggtac
     9781 actcctcgcc tttcttggaa taaactttaa agctgcctgt taccgtccga cagggtctgc
     9841 caacgtcctc acaaaaatct tttccccctg aagcactacc ggttttaact cggttaccct
     9901 gggcatcgta agctgcccag gcagtagctt taggatcaaa aataaatact ttttttcctg
     9961 tggcctgtct tttctcagga aagtaattag aaccctttct atcggactcc atactcatag
    10021 tgtaatgggt ataacccgca tcatcgacta tggccgttga tctatccatg gatgcacaag
    10081 aggcagccaa gacacaaacc ggaactaaca gtatttgttt tttcactttc tatctccctc
    10141 tttctctttt taactatatc gggagcagtt tgaataagtt taggcttttt tataaaaatt
    10201 ttttactgtt tgtattcttt atgatctaac aggttgttta atagtccttt ttgagcctgt
    10261 ttgggaacca tcgcaatgat agctagaatc agctgtttat cagtacattc aaagcatcct
    10321 cctctgggag gcatggcatt aaacccttca tcggtgtgtt taaaaagcac ttccattcct
    10381 tgtttcaatc ttgcccccca tgctctttca tttccaattt caggggctcc taaagaaatc
    10441 attggtttcg ttccatggca attaacacaa aaatgattaa aaattttttc cccttcatcc
    10501 tttgtgccag caattgactt tagaaactct tgaggatgat gattctcaga gtaccctggc
    10561 atcgcaaaca taaataaagc caatatacaa acaaaataac aaggtctcat gatatactcc
    10621 taaaattggc gcaatacttg cttgggacac ggtgcgtttc aatataaaaa atcaatagac
    10681 taagggagtt tatgtcgtga aaaagtgcat actaagcttt ttcttatttt ttgcaagcac
    10741 cagtttattt tcagaatcaa gtcagtctct cgaattatta aaaaataaaa tagaacaata
    10801 cgccctcaat gaattatcaa actatactga aggtaaaatt caggttacgg cggataatat
    10861 tgatcctcgc ctaaacttaa aagtttgtga tgataataaa cttgaagtat ttaaccctta
    10921 tcaaacaccc atgctcaata ccagtaccat gggaatcaaa tgtttggagg aaaacaatca
    10981 ctggacctta tatatacctg tcaaaataac tatatttaaa tctgtacttg tggcaaaacg
    11041 agctttactg aaaggtacaa aaattagtaa ttcagatata tatcaaaccg agcttgatgt
    11101 tcaaaaactg aaacaaggat attttactga cagtaaagag cttattggat tggtttgcaa
    11161 gcatgatatc acgcctaata gcccactaaa ccctttcaat attgaattag ccaaactggt
    11221 ccataaagga gaacaggtat ctataatagc cgctcatgac aacttaacag tgagtatgga
    11281 tggaattgca atggatgagg gagcgctagg agactctgta aaagtaaaaa atctttcctc
    11341 aaaacgaatt attgaagctc aagtcacagg aaagaaaacc gtgaaagtta ttttttaact
    11401 aatgttttaa tttttaagaa aaaatttgga aaaaggatta aaaatcaacc ttaatttact
    11461 aaagaaattg caaaaggagc cgataatcat gataaatacg attgaggata ttcatatggt
    11521 taaccaaatt aatgactctg ccaatttaag gcatatcgat atggacaatc gtatcaatgc
    11581 aaatcacaag gaagtcaaaa gccctgttct ggaaaataac tctactgata gcgtcaattt
    11641 aagtagtaca tcgaagcaac tggaagcttt aaaagcatct cttaaagatt tacctgaaat
    11701 caatgaggct cgtgtattgt atttcaaagc tgaaattcaa tccgggcagt atgaaattga
    11761 tagcagcaag attgcacatg gcatgttaaa tagtgtagag atggtttaaa ctgcagggcg
    11821 acccgatgaa taataacaat aaagcaaaaa cattaataac ccaattagaa caggaaatta
    11881 gctgggtaga agaactcaat actttattat ctgaagaaaa agagatactt tctaccagtc
    11941 aattcaatca gctggagaca ttagctgaga aaaaacagga attatcaact aaattagagg
    12001 ctagtgcaaa acaacgtgta gatcttataa aagaatcaaa tcccggcgac aaacctcatg
    12061 gactcctgtt gtctgatttt ctcaaaaatt gcgctacaga agatggcatt caaatcaatc
    12121 atttaaataa tactcttgca gagaaattaa acctatgtcg tgagttaaat accattaatg
    12181 gacaagtcat agcgaataac atgtataccc gtcaacaaat tgtcaacgcc ttgtcgggaa
    12241 ataaatctaa tgctgtgagt gtttacactt ctaacgggaa tttggaaacc ccttccgaaa
    12301 gcagtcacca tgaagaagca taagaaatat aacccctagt attttttaaa atagctctcc
    12361 agtatgatgc gccaggcata gccgctcaac gcatacggag agccgcatcc aaacgaatta
    12421 cctctccatt aatcatctgg ttttcaataa tatgacctac tagtgaagca aattcctctg
    12481 gtctgcccag gcgtttggga aaagtcactg ttgcaaccaa actgtcctga acttcttgag
    12541 gcatatttaa aagtaaagga gttgctatta accccggcgc aattgtattt actcttattg
    12601 caaattgagc taattcacgt gctgctggca aggtcattga tactattcct ccctttgacg
    12661 cactataagc agactgtcct atttgtccct caaaagcagc aatggaagct gtattgatta
    12721 tgacaccccg ttcctgagat ttgttgtcta actccaatcc tgacatggca tgtgcagcta
    12781 ttcgcatgac gttgaaggta cctattaaat tgacatctat tacttgtcta aatgattcca
    12841 aaggcattgg cccttctttt cctaccatgc gttttgccgg agcaattccc gcacaattga
    12901 tacaaaccct tggtactcct acctgagaga tagtttgtcg cattgccttt tcaacagatt
    12961 catcactggt tacatcacaa gcaacataca attccgcctc atttgaggtg ccttcctgtt
    13021 tatcccagac cacaactttc attccatgct gttgtaagta ttgtacacaa gccttaccca
    13081 tacctgagac accaccagtt actagtgcaa tctgattatc gaaattcatt ttctctccaa
    13141 aattctacgg cttcaatttc caactttctt attctactta aaaacagtat tctattagac
    13201 ctcttggcaa acctgcttat tttttaaggc aaagcacaaa accgctttga ggaaggaagc
    13261 ttacatacag tacatgagta ccgcagaaac gtttgcaaca cagcaataaa aggaaataat
    13321 caggccatgc aaatggtcta ttgtaaaaat tctttgctaa tcgctttaaa ttctggcgta
    13381 cctgcttgtt ttaaccactc gaacagtacc atttcagcgg ttatcaaatg gatcccattc
    13441 tgtttcatcc tttttagacc gtattttaaa tcaaactcag agcgactgct cacaccatct
    13501 acaacaacaa agacctcgaa tccatagccc ttcatttcta tggccgtttg aagcacacat
    13561 acatgagcct caattccgat aagaataagc tggtttttat tatatccttt caggaggtta
    13621 atatattgtg gttcttgcat gcatgagaaa tgaatttttt caatgcattc ttcttgattg
    13681 atataagagc ttaaaggaga aacggttcga cctaaacctt tagggtattg ttcactagct
    13741 agaacaggca cgtccagttt tctggctaat tttagtaacc attcacatcg agctaaaaaa
    13801 agatcgagat ttaatattgc cggggttagt ttttcctgta catcaatcaa gaccaagaga
    13861 gaatccgctc tatttagtag taacatcctg ataacctcat tctatttatt gagacatatc
    13921 atatagcatt aagaagataa actaaatgga gtaacctgaa ttatacaaga cttgaagcgg
    13981 agtaaatccc ggattggata atacttcaat ccgggcaaat aatagacaga aaaaagtaac
    14041 gagagttaaa gggctgcaat atcaattgtt ttcattttga catccatttt cttaccattt
    14101 cttaatattg aaacagtaat ttgctctccc accttgattt ccgtaagcaa attatataaa
    14161 gcatcgtaat ttggaacagg atgcgcattc acgcctacaa taacatcacc caaaacaata
    14221 cgcccccatt gatctctatg agtgcctctt aatttcaatt tgtcggcagg agttccgggc
    14281 accacatccg caatcaaaat acccttttta acacccaatc tttgagcaag atgtggctca
    14341 actcgctgaa taccgatacc agataatacc actcgaccat gattgataat ttgcgatgca
    14401 attttctgaa tatcttctgc cggaactgcg aaaccaatac ctgcagaaga acctgatcgt
    14461 gaataaatca tggtgttcat accaattaat tggcctgcgc tatttaatag aggacctcct
    14521 gaattacccg gattgatagg ggtatctgtc tggatcatat cgtatattgt tacaccgcct
    14581 atgcctggta cttttctacc taaagcagaa ataactccct ttgataaact gtgatccaag
    14641 ccaaaagggt ttccaatcgc aatcgctttt tgacctacga tcaaatcgtt aagtgaaact
    14701 atttcaaagg gttgaaatga tttcaaataa tccagagcct tgggtgattt aatttcaagg
    14761 actgcaatgt ctttgcgagg ctctgaacca atcactttgg cagggacagt catatttccc
    14821 aaagtgattg ctatgtcatc agcaccgtta atgacatgaa agttagtaac aacatggcct
    14881 ttgttatccc aaataatacc ggagcctgcc ccatcaggaa catgtgtttt ttgtaatgag
    14941 taacgacgct gaacagtagc atttgctaat cgatgaacat aaacaacttt agatgacgct
    15001 ttttgaaata cttcaaccgt atttcgttca tctggaagaa gagcatccag atttaaacta
    15061 aaaacaggtc tggccaggat aaaacataaa ataaaaggga taagtaataa gttacgttgt
    15121 tgtctcatca tctccattta actccatacc tgcacggctt gcaaatctct tgcgaaatgc
    15181 ctgtcgcgct aataatttat caattactga ttgcggaaaa tctgtaatag taatgatatt
    15241 tttatctatt aaaacatcaa tcaaatcttc aagtacacga atcaattgta aatcagactg
    15301 tagaaatttt aattttttgt cgctctcgca acgcaacaaa aaatcgacaa actctgcatc
    15361 attaatgtca atttgttccg ttacacccga ggtcttctgg ctatatacag cacaaatctg
    15421 accatcctta tcacgtttta tgtagaccac tggttaattc cttcttttag ctgttgctta
    15481 aatctagaaa aaacagacac ataaacctta tgtctgctgt ttctaaacta aagtcttctg
    15541 tattgattgt aagcctatac ctgaacaaaa gactttcatg agttattcaa ggaatatcat
    15601 taagtccaag catggcattg taatatattc tttcacagat gtcactaatt aactcaagag
    15661 ggtaattttt acgcaaacat taatcttgag tcattttcag aatccgactc atcttgagaa
    15721 tcttcattat ctgaacgatt agactcatcg tcagaattga catccgtatc actataaatc
    15781 tcatcgtcac tattttcaga atcattgata tcactttccg ttttatcaac atcatctcgc
    15841 gcagcattct ccagtttgga ttgatcaaaa ttaatacctg acgaaatatt aaaatattgc
    15901 agcaagaatt gtaaaggtgc caaaataatc gccttgaatg aagtagttcc agcatcaata
    15961 ctttgtttgg caatattttc actgtcatca gtatgccgaa tatatatttt ttctaaaagt
    16021 gatgtatgtt gcttgtaaag atgatccaga ctctcattgc gtgatttttc ctgacccaac
    16081 aaatattcct gtaaatccgt atgagtatcc cacaatgagg ttcgtctttg ttgcagatgg
    16141 agtaattcac tgtcatagaa ttttatttct tcatctatct ctttctttaa cctgtcacag
    16201 gcatcctttc gttctatcga gatccccccc tcaactatgg cacctgattc cttgagagat
    16261 acttgcgcat accgctccac taactgtgct tttttacgta aaacatcctc aattttatta
    16321 tccaggtaga taatcgcatc tttattcgcg ccgagaactt tataatgaga gtcaaatcgt
    16381 ttgttacttt gcatttgctc tctcattttc tccaattcac cggcttcctg atttattgct
    16441 tttctttgag cttcatcatt taaattttta tctctgctta ataatagctg tctgataaat
    16501 gcggataatg caacaactgc tccaaacgac gccgcacaga tgagggctat agtcactggt
    16561 tccatagtca actccttaag agtgcaaatg tatcaattgg gtgtgtattt caacagcaaa
    16621 ataccggctg aaagactgaa atgaattgac ttgtggccat tgtgttggtt gaccgcttac
    16681 ccggctcata gtcgtttcaa atattggctt aaaatattgc tttataaatg gctttacatc
    16741 tttgatggct gtaaaatttt taacaattac cgttgagttt tctgctatct gagccaggtt
    16801 tacccgactc ataacatcag aaagctcctc ttgatcttca acagtacatt tcatccattc
    16861 taaaagcgct gaacgaggct taacatttaa catctggcgc ccttctttta aagagggttc
    16921 cattaaaata atatgggaat gcaactgaaa tttaaaacaa cacagaaaat caagaaaact
    16981 cgtttcaatt tcattatgtg cggaacgacc taaccaacga caaatttcat gtctgaacat
    17041 tgtagtgaaa tgcttttcga tctcattaag agtatcttcg tcagaatcat acttgggaat
    17101 aacataagcc gtattgtcag tatgcaataa ttttaagcta ggtaaattag cttctggtaa
    17161 ttgtgacgct agaaaagata aaaatagatt agtaggcttc aatactacta tctcggattg
    17221 tttttgatgc atgaggctct ccttgattta attaactcct cctgattaca tctcaactat
    17281 taaaatcaac gtctcatctc aatttagaat cttagtcatc actttgacta atacataata
    17341 cagagttttc ttcacaaaca cacatgaagt catatttgac cttcaaagac ttaaaaattc
    17401 cctgatattg ttaaatttag gaccaagatt agaacatcaa caaaaaatct ggtaatacat
    17461 taactcataa gtccaaaaaa aaacctctct tattcagtct aatttgccta tatgatagtt
    17521 tcaatagtta aacagaattt ttttgagaat attttcccaa tttgttagaa tgcataaata
    17581 tagtcttaaa tccttcttga cagaaactcc tttatctata tgcttttgtt tttaaactct
    17641 ggttctatcc catgaaattg ctaggaaaaa ttctacttgc ctttactgct ttaatgttct
    17701 tcgtatctat tactttatgg attttagcaa aaaatattaa acccgaaacc attaagcaat
    17761 tagtcagtaa taaaatcaca gcaatcactc acaagcaaag ccatattgat gggactattt
    17821 cctggcaatt gttcccaaga cctggactca aattcaccaa gatacatata ggtgatgaca
    17881 atttaaatga caattattct cttctgattg ataccttgct attaaattta aaaattaccc
//
LOCUS       DATNSN010000054        17863 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.054, whole genome shotgun sequence.
ACCESSION   DATNSN010000054 DATNSN010000000
VERSION     DATNSN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.054"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..158
                     /locus_tag="VAH76_001500"
     CDS             <1..158
                     /locus_tag="VAH76_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1386132.1"
                     /translation="LLMSHVTLSIGIVYIHFESSVSIEHAITAADKALYQAKKKGKNQ
                     IVVKKLL"
     gene            complement(220..861)
                     /locus_tag="VAH76_001501"
     CDS             complement(220..861)
                     /locus_tag="VAH76_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386133.1"
                     /translation="MKMKLQLIAIGILLSINVFADGYYPEPTSQDQPWTIIASFGSGR
                     YQHMYSKDGKTALGRLALANELMLSGDIALGLELGVQNGNKMRLNIPRETLSVLGWLP
                     VETNLSPMLDLLVTAKSDPLFGSSFFAQLKGGVAYRSWQVDLYPINEISQLAGEIQAG
                     FGYPITALASLSLLYQGVFGNDPNVKLNMSSKTGSVSNIPVLHGILLGFTVNL"
     gene            complement(1034..1121)
                     /locus_tag="VAH76_001502"
     tRNA            complement(1034..1121)
                     /locus_tag="VAH76_001502"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1085..1087),aa:Ser,seq:gga)
     gene            1344..1778
                     /locus_tag="VAH76_001503"
     CDS             1344..1778
                     /locus_tag="VAH76_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386134.1"
                     /translation="MTLQHSIQKGVSDFSFIIGTGLKYGGAGGLLVMAVVGTVAIDLV
                     LLAAAEKHHNSFLTGFVLGSMFSRGNLDPVPLLIASPITSAVAVVLSVALGVPGVGLA
                     ILAGWTLAGTLLAVGMGLEAFAKSIDPELSSESDYNSCLCFN"
     gene            1900..2346
                     /locus_tag="VAH76_001504"
     CDS             1900..2346
                     /locus_tag="VAH76_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386135.1"
                     /translation="MYLRLLALSALCFVTSPIWSFTCIYTLVKDNCWTDYDVTVDIIE
                     DSTSKTLLTVTAPKGKSWARGTFNCEAAEGLRYVAQFSPVFWQNDVGKTYPALRNWYL
                     PAKVNPGDLAWTIPVCFPADFAQVPFPPNVAGNCKCNFKNIPDPKL"
     gene            2474..3550
                     /locus_tag="VAH76_001505"
     CDS             2474..3550
                     /locus_tag="VAH76_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEO1386136.1"
                     /translation="MITIRNKIGQMLIMGFHGSELHDNSPVAQWLSNDGLGGVLLFDK
                     DLSTGIYGKNLRNQAQIRQLIRQLNRYASMISGGNDNLPLFTAIDYEGGVVDRLSKIE
                     GCPPTMKARELAKLSSDDFDEEVTQMAMTLQSLGFNLNFAPVVDLNLNAHAGIIGNLG
                     RSFSSNPEVTIRLAKQFVDVFSRYGIACAYKHFPGHGSATGDTHEGFVDVTETFQTDE
                     LIPYRSLLKDLSNPVMVMTAHVINKHLDSKGLPATLSYDVLTLLLRQSIGYDGVIISD
                     DLQMHAISNHYSLEEALCLTINAGADMVIFANQLGTITAPEVIDVIEKLVIDKQISSQ
                     RIDEAYRRIVRLKQQIICSELINT"
     gene            3937..5217
                     /locus_tag="VAH76_001506"
     CDS             3937..5217
                     /locus_tag="VAH76_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEO1386137.1"
                     /translation="MCAAHHQKNKYIFSTPLIYAAMIGLGIISGLSDLPFLKETGLLI
                     SDLFIKLFKCISLPIISLSIIVTLANYQTDSLMKSVWQRTIKYTFATTLVAALISCFL
                     YILIHPSTVQVNLDAANPKSTSSLGYLGYLSNMIPTNLLSPFLEQQVMGVLLLSIVIG
                     FAVRQIPETEPRETIIRFFRGAHGMFLVMTKWIIKLIPLGLFGFITSTVIQLRSGMDI
                     KGIGQYLMIIVLANLIQGLVILPLWLKSNGIKPFSSMRAMLPALSVAFFSKSSVGTLP
                     VTMNTVEANLKVNPQVSRFVLPLCTSINMNGCAAFIFATVIYLMQSHGIQVSYATMGI
                     WVLVSTIAAIGNAGVPMGCFFLSVSLLSSMNVPITLMGVILPFYGLIDMLETALNVWS
                     DACVTKVVNDKAEGGDGVVGIRPKERSLLNAELG"
     gene            complement(5540..6673)
                     /gene="dapE"
                     /locus_tag="VAH76_001507"
     CDS             complement(5540..6673)
                     /gene="dapE"
                     /locus_tag="VAH76_001507"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEO1386138.1"
                     /translation="MTDITQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNN
                     GPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTDPFSLEEKNGMLYGRGVADMKG
                     SLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
                     VGEPSSSLKTGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSM
                     QWDNGNAYFPPTSMQITYIHCGGHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAF
                     THHKLNPTIEWRFNGEPFLTNKGILLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPY
                     GVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLFID"
     gene            complement(6666..7496)
                     /gene="dapD"
                     /locus_tag="VAH76_001508"
     CDS             complement(6666..7496)
                     /gene="dapD"
                     /locus_tag="VAH76_001508"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEO1386139.1"
                     /translation="MNSLQALIEQAFENRQNLSLNTVSSDLINAINEVLSGLDNGQFR
                     VAEKINGEWTVHQWLKKAVLLSFKLFPNQIIDAGFCKFYDKIPLKYTDSSNEQFQQSG
                     VRVVPHAMVRRGAYIAKNTVLMPSYVNIGAYIDEGVMVDTWATVGSCAQIGKNVHISG
                     GAGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEKNSVISMGVFLGQSTKIYNRI
                     TGEVSYGRIPAGSVVVAGNLPSHDGSHSLYCAVIVKQVDEKTRAKVSINDLLRANQDD
                     "
     gene            complement(7500..8393)
                     /locus_tag="VAH76_001509"
     CDS             complement(7500..8393)
                     /locus_tag="VAH76_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1386140.1"
                     /translation="MQKNKQNGGQLHAIAAIFALIVSTTLCFIPILFVGLLKLFPNKN
                     WQSLCTRCVDRIATFWCGINNFYVAKAQKIHWEISGLNNLTPKDWYLVVANHQSWLDI
                     VILQRLFNRKIPVLKFFIKDQLKWIPLLGFSWWAMGCPFMKRYSKEYLAKNPHKQGKD
                     IISTRKAIETFKRTPASIMNFVEGTRFTKVKKEQQQSPYNYLLKPKAGGISFIINSMG
                     QQIKSLIDVTIVYAEKNHSLWDFLCKRVKKVKIIIRQLPIPAQFTSANLINDFQAQNA
                     FKEWLNEQWAIKDNLIASLQE"
     gene            complement(8460..9611)
                     /locus_tag="VAH76_001510"
     CDS             complement(8460..9611)
                     /locus_tag="VAH76_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEO1386141.1"
                     /translation="MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLG
                     YEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAAINTVIDLLDSGDHVLAMDDLYG
                     GTFRLFDKVKTRTSNLSFSFIDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
                     AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNP
                     MLSDKIAFLQNSCGAVAGPFDSFLVLRSLKTLSLRMQRHCENANHLANWLNGHPKIEK
                     VIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARCELFTLAESLGGVES
                     LIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG"
     gene            9760..12075
                     /locus_tag="VAH76_001511"
     CDS             9760..12075
                     /locus_tag="VAH76_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1386142.1"
                     /translation="MRRQEINYEKVAKAAEKIKKRGIEPSVNEIRDELGLVGNHPQLS
                     ILLEEWYHNQPEFKRKSHTPLTENINLNTDEIREKNVELEKSISLLRATLESTADGIM
                     MVNGHGAVVDWNQKFVEMWRIPSYMMESGKESISFEYILEQLIDPQSLIADVQYLYQN
                     LEWQGELPELHFKDGRIYERFTQPQRVGSQIVGRVYSFRDVTQKRMALDELRIRERAI
                     EASTHGVVIIDVTKNENKVIYVNRAFERITGYGEQHALGKGLLTLLGSNLEEVNHKRI
                     ELAIRESKEETIEMESIKRNGEFYWCEISVAPVKDSFGYVKHYICILNDVTQRRDMED
                     QLLQQATYDSLTNLPNRVLLMDRVEQAILQARKNKAILAFLFLDLDRFKLTNDTLGHS
                     MGDKLLQAIANRLLIVTEDFDTVARLGGDEFVILLTDIDNMLEAETIAQNILKIIEKP
                     IQIDQHSLKITGSLGISFYPRDGDDYESLMKSADLSMYHAKDTGRNNYRVYEPEMNRR
                     VINHMQLDNALRDALKNDELFLVYQPLIDLKQSRVVGFEALMRWHSKILGLVIPADFI
                     PMAEENGMILEMGEWAMKQACIQVKEWHKAGFKNLSIAVNLSGRQFRQKNLPEVVSRV
                     LKSSGLQSRFLELELTESLLIEDIDHVVDTMYALKDMGTKLVIDDFGTGYSSLSYLKQ
                     FPVDKLKIDRSFITEMVSNQNDAAIAKAIINLGHSLNLQVLAEGVENEFQRDFITSHG
                     CDYAQGYFFKAPDTPENILEFLKSLSESKIK"
     gene            12377..13726
                     /locus_tag="VAH76_001512"
     CDS             12377..13726
                     /locus_tag="VAH76_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEO1386143.1"
                     /translation="MKHITIIGAGLAGTLCGLYLARRGYEVELFESRPDIRNSPTDYG
                     RSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEVHYQPFGRHIDEYINA
                     ISRSDLNALLLNKAELCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEASYHRLIGA
                     DGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLG
                     NPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPT
                     GHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSR
                     VTPVFYEQRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMH
                     VLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLSIYDKKLAKII"
     gene            13944..14696
                     /locus_tag="VAH76_001513"
     CDS             13944..14696
                     /locus_tag="VAH76_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386144.1"
                     /translation="MKTVQAYIDSKQHEFMNHPFFNILEELNSIEEISYFVPELTFWA
                     MTFQDILRINEERVTDPYLKKVARHHRLEDAGHEKWFLSDKKYMGEICDNKACDRDDV
                     AWLYSKDSQLVRDAAYSIMSEIYKIEDENLNIVLLLTLESSGHVFFEKVVKQVKKTGE
                     DKNLKYFSSSHLEVELAHALFEEEMERKVYSRRLPISTRREALRMVDRCYDAFSKMFD
                     GLILACNKRLELAKERNQHVAKSVELRENQAV"
     gene            14653..16023
                     /locus_tag="VAH76_001514"
     CDS             14653..16023
                     /locus_tag="VAH76_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEO1386145.1"
                     /translation="MSQNQWSLEKIKQYKEDTGQALLSDEQSLVLFGQDFGKLIQSQP
                     VAVSAPQNIESLQSLILFANQYHLPVTIRGNGLSQGGQSLPVPGGLTISMQSFNKPLD
                     LSEDLIWVEANTSWKNLLEKSLLKNKAPYVLPYNCNLSVGGVLSAGGIGASSFKYGVI
                     NAYVSALEVVDGLGRKQIVEKNSPLFQACLSGQGRFGVITKACIQLRSVQPRVKTFFL
                     VYADQNQWFEDIYKIQGKVDYMEIFCSPSIQGARLKEDKRIPMAYWLYGLHLSVEYDQ
                     HVEDILGQLKPWNVLNIQEESILSYFLRHDSRFDMMKLTGQWDLLHPWYECFVPTSFL
                     KGILSQLLEELPLHYASLVHVVPIAQKKAGFMMLPDSDSICEFMILNPGVPSSLAESC
                     LQAIESLDKYLLQNNGKRYLSGFLGTELPDNYWLKHFGEKYDSWAGLKKQYDPAGIFS
                     SVLHQI"
     gene            16370..16879
                     /locus_tag="VAH76_001515"
     CDS             16370..16879
                     /locus_tag="VAH76_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspC"
                     /protein_id="HEO1386146.1"
                     /translation="MKFDIQFLFSANYAKWIIISLVSLFGILIIYEWSTLFYPLTSVK
                     AIPGSNTKSIAGMKEENLNYLFNTSLFGVYVPADLNEDNVKQSMLNVTLVGILFADKI
                     EESQVIIRSASGEEKTYYVGDKIPGGATIKRIMPGGVLVERDGTLESLSLPKNDLTFE
                     PVAKPLKEE"
     gene            16885..17262
                     /locus_tag="VAH76_001516"
     CDS             16885..17262
                     /locus_tag="VAH76_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEO1386147.1"
                     /translation="MQRIFKLFILIIFIPMMAACITGTLNLREGIQCFKAQDYRKAFI
                     RLKPEAQKGNPDAQYAVGYMYYYGKGVVEDRKKARFWITCAARAGQPDAIVALGILDR
                     QARQDRRLFEDNNLQHFPVDNGL"
     gene            complement(17385..>17863)
                     /locus_tag="VAH76_001517"
     CDS             complement(17385..>17863)
                     /locus_tag="VAH76_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEO1386148.1"
                     /translation="SILSCRSTPANLLSIAIGLLILPICSKYIVYNASEIAKWAGISE
                     FTIGLIIVAIGTSLPELTTALVAAVKGEDSIALGTILGSNIYNMLLVLALPGLLNPTK
                     VSSVVLWRDMPVMLSITLLLLFLNYYYQKKTSSWPGGILLLVYFCYMASLIINAHG"
BASE COUNT         5483 a         3044 c         3646 g         5690 t
ORIGIN      
        1 aattactgat gagtcatgtc accctaagca tagggattgt ttatattcat tttgaaagtt
       61 cagtcagtat agagcatgca attactgcgg cggataaggc attatatcaa gctaaaaaaa
      121 agggaaaaaa ccaaattgta gtaaaaaaac tactttaaga agatgacatt ctatctttaa
      181 ctgagatggc gcctggaagc tgcgtcctag tttaatggat tataagttga cagtaaatcc
      241 taataaaatt ccatgtaaaa cagggatatt tgaaacactg ccagtttttg agctcatgtt
      301 taatttgacg ttaggatcgt taccaaaaac accctgatac agcaaactca aactggccaa
      361 agcggtgata ggataaccaa agccagcttg aatttctccg gctaattgtg aaatttcatt
      421 gatagggtag agatcaactt gccaagacct gtaagcgaca ccacctttta attgtgcaaa
      481 aaaagaactt ccaaataaag ggtcgctttt ggcggtaact aataaatcca gcataggact
      541 gaggttggtt tctaccggta gccatccaag gactgataag gtttctcgtg ggatattcaa
      601 gcgcatttta ttgccgttct ggactccaag ttctaatccc agagcaatat ctccactcag
      661 cattaattca ttagccagag ccaatcggcc taaagcggtt ttgccatctt ttgagtacat
      721 gtgctggtat ctgccacttc caaaactggc aataattgtc catggttggt cttgagaggt
      781 gggctcagga tagtaaccat cagcaaatac gttgattgat aataaaattc ctattgcaat
      841 tagttgtaat ttcattttca tctttcatat ccctgattga attggttggt tctgaaagca
      901 gttttaccaa aagaatctta tatgagccag ctgtagtctt tacacaatgg atataattta
      961 ctgctttatg caactcaatg aaagcggcaa ttatagcctt ataccctgtg aaggataacc
     1021 aggaattgta atttggcgga gagagaggga ttcgaaccct cgatacgttg ccgtatacac
     1081 actttccagg cgtgcgcctt cgaccgctcg gccatctctc caaccgggca agcttatact
     1141 tgtcggcaaa taaaagcaag actgtgataa ttattaaaga ggttttccat cacaaagata
     1201 atatattcta tttttggatg ggaataaatt gtatcgcttt atttgagttt taatccaatc
     1261 tactcgttac ttcagtgttc aattagtgtt gattatgtgc aaaattaagt gtattattcc
     1321 tcaatttttt aggagtaaga ttaatgacac tgcaacattc catacaaaaa ggggtatctg
     1381 attttagttt tattattggc acaggtctaa agtatggtgg tgctggcggt cttttagtga
     1441 tggctgttgt aggaacggtt gctattgatc ttgtcttgct tgctgctgca gaaaaacatc
     1501 ataacagttt tttgacaggg tttgtgttag gttcaatgtt ttccagaggt aatctggatc
     1561 cggttccact tttgatcgca tcccccatca catcagctgt tgctgttgtg ttatctgttg
     1621 ccttgggtgt tcccggggtc ggtcttgcga tactggctgg atggactttg gctggcactt
     1681 tattagctgt tggtatgggc ttggaagcgt ttgccaaatc cattgatcct gaactttcat
     1741 ctgagtcaga ttacaattct tgtttgtgct ttaactaata gagtcaagag gggaggtcag
     1801 attatttccc ctcttaaaac agtttatcgg aaaattattt ttataaatat tgtatatact
     1861 atatagtaat aatggttcat taaatcttag gattgaatca tgtacttacg tttactcgcc
     1921 ctatctgcat tgtgctttgt gactagccca atttggtctt tcacatgtat atataccttg
     1981 gttaaagaca attgttggac tgattatgat gttactgtcg atatcattga agattctacg
     2041 tcaaaaactt tgttgacagt taccgctccc aaaggaaaat catgggctag aggtactttc
     2101 aattgtgagg ctgctgaagg gttgagatat gtcgctcaat tttcgcctgt cttttggcaa
     2161 aatgatgtcg gaaaaaccta cccggcatta aggaattggt atttaccagc aaaagtgaat
     2221 cctggagatt tggcctggac tatccccgtt tgttttccgg cagattttgc tcaagttccc
     2281 tttccaccta atgtagcagg aaactgtaaa tgtaacttca agaacattcc tgatcccaag
     2341 ctttaatttt atgagaagca tgtttttatt tgtacacatt gccatactgt ttattgataa
     2401 tgcgtagcgg ggcatagcca gagagaatat ctttgtgtat gattttatgc ttattttgtg
     2461 atggagcgag tgattgatta caataagaaa caaaatcgga caaatgctca ttatgggatt
     2521 tcatggttcc gagctgcatg ataatagtcc ggttgcacaa tggttatcta atgatggtct
     2581 cggcggagtc ttgttattcg acaaagatct atccacaggg atttatggca agaatttaag
     2641 gaatcaggct cagataaggc aattgattcg acagcttaat cgttatgcat caatgatttc
     2701 aggaggtaat gacaatctgc ctttatttac tgcgattgat tacgaaggcg gagtggttga
     2761 caggctaagc aaaatagagg gctgtccgcc tactatgaag gccagggaac ttgcaaagtt
     2821 atccagtgat gattttgatg aagaagtaac tcaaatggct atgacgttgc agtcattagg
     2881 ttttaattta aattttgccc cggttgttga tttaaattta aatgctcatg cagggattat
     2941 tggaaacttg ggaagaagct tttcttctaa cccagaggtt actattcgtt tggccaagca
     3001 gttcgttgat gttttcagtc gttatggtat tgcttgtgcg tataaacatt tccccggcca
     3061 tggcagcgcc actggagata cacatgaggg atttgtagat gttacagaaa cctttcaaac
     3121 cgatgagcta attccttatc gaagtttatt aaaagacctg tctaaccctg tcatggtaat
     3181 gacggcacat gtgattaata agcatttgga tagtaaggga ttgccagcaa ctctgtccta
     3241 cgacgtttta acgttattgt tacgtcaaag cataggatat gacggggtca ttatcagcga
     3301 tgatttgcaa atgcatgcca tttcaaatca ctattcatta gaagaggctc tttgtcttac
     3361 catcaatgcc ggggctgata tggtcatatt tgccaatcag cttggaacta ttaccgcgcc
     3421 ggaagttata gatgtgattg aaaaactggt aattgacaag caaatttcct ctcaacgtat
     3481 tgatgaggca tacagaagaa tagtacgtct gaagcaacaa attatatgca gtgaattgat
     3541 caatacgtaa tgcccatgcc aagtggtagc tggcggattt atgaaccatc aagttaccac
     3601 cataacttca cctattagag aggctccatc ttcttttcag catgaacaga cataattgtt
     3661 tgagattggg tttgaagtat tttattagta tctatatggt gtgttctgtg aagaatataa
     3721 ttgacacagt ttatttatcc tattgactag aagaaaaatc gtgttacaat atgtactcta
     3781 aatgaacaat tttagctatg ggtgatgact tttatgtcaa ttcaatcatt atattattat
     3841 aattcctcac tatttcactc catatatcaa gacatctgtt gttgctgcta atcctacttt
     3901 gcttaaataa ttaattaatg ttttaaggat tccgttatgt gtgcagcaca ccaccaaaag
     3961 aataaatata tttttagtac cccactaatt tatgcagcga tgattggact tgggataata
     4021 agtggattat ccgatttgcc ttttttaaag gaaacagggc ttttaatttc tgatttgttt
     4081 attaaattgt ttaagtgcat tagtttacca attatttcct tgtcgattat tgtaactctg
     4141 gcaaactatc aaacggatag tttgatgaaa agtgtatggc aaagaacaat taaatatact
     4201 tttgctacaa ctttagttgc agcattaatc agctgttttt tatatatttt gattcatcca
     4261 agcactgttc aagtcaattt ggatgcggcg aacccaaaat ctaccagcag tttaggatat
     4321 cttggctatt tgagtaacat gataccgact aatttattat ctccattctt ggagcaacag
     4381 gttatggggg tattgctgct tagcattgtt attggttttg ctgttcgtca aataccggag
     4441 acagagccac gggaaactat tattcgattt ttccgcggtg ctcatgggat gtttttagtg
     4501 atgaccaaat ggataattaa attaatacct ttggggttat tcgggttcat tacttctact
     4561 gtaattcagt tgcgatcagg tatggatatt aaagggatag gccaatactt gatgattatt
     4621 gttttagcta atttgataca aggattagtc attctgcctt tatggctgaa atcaaatggt
     4681 attaaaccat tctcttcgat gcgggcaatg ttacctgcgt tatctgttgc ttttttttcc
     4741 aagtcatctg tcggtacttt gcctgttaca atgaatactg tagaggctaa tttaaaggtt
     4801 aaccctcaag tcagtcgttt tgttttacct ttatgcacaa gcattaatat gaatggatgt
     4861 gctgcattta tttttgccac agtcatttat cttatgcaaa gtcatggcat ccaggtttct
     4921 tatgctacta tggggatatg ggttttagta tcaacaattg cggcaatagg gaatgccggc
     4981 gttcctatgg gctgcttttt cttaagcgtt agtttgctct ccagtatgaa tgtgcctatc
     5041 acgcttatgg gagttattct tcctttctat ggtttgattg atatgttgga aactgcctta
     5101 aacgtatggt ctgatgcttg tgttaccaag gttgttaatg ataaagcaga aggtggtgat
     5161 ggagtggtgg gcattagacc aaaagagcgt tcattattaa atgccgagtt aggttaaagc
     5221 cagcaaatct tcaagcgcaa atgagtgtag cttacatata agtctccaga aagttttttt
     5281 gagacttata tctatgaaat cagtttgtaa tttataagag atcagctcac tacctggaac
     5341 ggccgtattt ggttcatcgc gaacgaaaat aatccttcaa aaatgctcac agaacaatct
     5401 atactgtgct ttttcaagga ttatttttat ccaaactaaa tgcaaatctg accgccagag
     5461 gatatttaaa gatgaggtta ctaacctatt actgtaattt ggactgttgg tgcgaaccct
     5521 cattacagaa ttggtttgat taatcaatga ataatttctc acagattgaa aaatacatgg
     5581 tttcaagtgt attcaaatcc tgtagagaag tgcattcatt aacctgatga atggttgcat
     5641 ttaccagtcc cagctctatg acctcaaccc cataaggtgc tataaaacgc ccatctgaag
     5701 ttccaccact tgttgaaagt tcggggagag tcccaatatg ttcaagcact gtttgtttgc
     5761 agctttctag caatatgcct ttattcgtta agaatggttc gccattgaat cgccattcta
     5821 tagtggggtt aagtttatgg tgtgtaaagg cattaattac tctcgttttt aatgactcgt
     5881 ctgtttgctc tgtcgaatat ctaaagttca aatgcagatt taattcgcct gggataatat
     5941 tacccgcatg ccctccacaa tgaatataag ttatttgcat ggaggtagga ggaaaataag
     6001 cattgccatt atcccattgc attgaagtga gttcggctaa tactgggcta attctatgaa
     6061 ttggattgtc agccaaatgc ggataggcaa catgaccttg ctttccactc agatgtattt
     6121 ttgcacttaa tgatccacgt cttccaattt tgatgacatc accagttttc aagctgcttg
     6181 aaggttcacc tactatgcaa taatcaataa caatcccttg ctgctccagt ttttgcatca
     6241 cataaggggt gcccaaatta aattcatcgc cttcttcgcc gctggtgatc agaaatccta
     6301 gtctgcctgg aaaggaagga taagttttaa taaatcttct ggccatatgc aacatacagg
     6361 ccaggctgcc tttcatatca gcaaccccac gaccatagag catgccattt ttttcttcga
     6421 gagagaaagg gtctgtatcc cattttgata cttcaccaac aggaactaca tcggtatggc
     6481 ctgcgaacac caataaaggc ccaattttgc catagcaggc aaaaaaattg gatacgggcc
     6541 cattattcag ttgttgacaa gtaaatccca attgctccag aaattgaatc atgtattttt
     6601 ggcagccagc atcttctggt gtaatagaag gaaagccaat gaggtcagtc agaatttgcg
     6661 taatatcagt catcttgatt ggctcttagt aaatcattaa tgcttacctt cgcgcgagtt
     6721 ttttcatcca cttgtttaac aatgacagca caatataaac tgtggcttcc atcgtgactt
     6781 ggcaaattac ctgcaacaac tactgagcca gccggaattc tgccgtagct tacttcgcca
     6841 gtgattcgat tgtagatttt agtgctttgg cctaaaaata ctcccattga tatgacggag
     6901 tttttttcta cgataacccc ttccactatt tcagagcgag caccaataaa gcaattgtct
     6961 tcaatgatgg tcgggttcgc ttgtaatggt tctaaaacac ctccaatacc cgcgccgcca
     7021 gatatatgta cattttttcc tatttgggcg caagaaccaa cagttgccca ggtatcaacc
     7081 attactccct catcaatata agcacctata ttgacataag aaggcattaa aacagtattt
     7141 ttggcaatat atgctccacg tcgcaccatg gcatgaggaa caactcttac ccctgattgt
     7201 tgaaattgct cattactaga atccgtatat tttagcggta ttttatcgta aaacttgcaa
     7261 aaacccgcgt ctatgatttg atttgggaaa agtttaaagg ataagagaac tgccttttta
     7321 agccattgat gaactgtcca ttcaccattt attttttcag cgactctgaa ttggccatta
     7381 tcaagaccac tgagaacttc attaatggca ttgatcaaat ctgaagaaac tgtgttgagc
     7441 gataaatttt gacggttttc aaaggcttgt tcaattagag cttgaagtga gttcatgttt
     7501 tattcctgta aagaggctat taaattatct tttattgccc attgttcatt taaccattct
     7561 ttgaatgcat tttgtgcctg aaaatcgttg ataagatttg ccgatgtaaa ttgtgcaggg
     7621 atagggagtt gacgaataat gattttaact ttcttgactc gtttacaaag aaaatcccag
     7681 agagaatggt ttttttctgc ataaacgata gtgacgtcaa tcaggctttt tatctgttgt
     7741 cccatcgagt taataataaa actaataccg cctgctttgg gtttcaataa ataattataa
     7801 ggggattgtt gctgttcttt ttttacttta gtaaaacggg ttccttctac aaaattcata
     7861 atagaagccg gggttctttt aaaagtttct atggctttgc gagttgaaat tatatctttt
     7921 ccttgtttat gagggttttt agccaaatac tccttactat accgcttcat aaaggggcaa
     7981 cccatagccc accaggaaaa gccaagtaga ggaatccact ttaactgatc tttgataaaa
     8041 aattttaaaa cgggtatttt tctgttaaat aaacgttgaa gaatgacaat atccagccaa
     8101 ctttgatgat tggcaacgac taaataccaa tctttaggag ttaaattatt taacccggag
     8161 atttcccaat gtattttttg tgctttagcc acataaaagt tatttattcc acaccaaaaa
     8221 gtagcaatcc tatcaacaca acgagtgcaa agggattgcc agtttttatt aggaaataat
     8281 tttaataatc caacaaataa tatggggata aaacaaagag tagtggacac tatcaatgcg
     8341 aaaatagccg caatcgcatg gagctgcccg ccattttgtt tatttttttg catgaaatac
     8401 ttctcctata cacatctgtc ctggttcttt ttaacagtca gaaccaggat ttacatcttt
     8461 taacctaaag catgctccag atcggcacgc agatcatcaa tgtgttcaat accaactgat
     8521 aatcttatga aaccatcttc tatacccagg gcttttcgtt gttcgactgg aatagaggca
     8581 tgggtcataa ttgcagggtg ttcaattaaa ctttctacac cccccaggct ttcagccaaa
     8641 gtaaataatt cgcagcgagc taaaaatcgt ttggcatctt caagaccacc tttaagtacc
     8701 aaagagatca taccaccgaa atcgttcatt tgttccttgg caagagaata ttgcggatga
     8761 ctttttaatc cgggataaat gactttttca atcttggggt ggccgttaag ccagttagcc
     8821 aggtggttag cattttcaca gtgcctttgc atacgaagag ataatgtttt taagcttctc
     8881 agaaccagga aactgtcgaa agggccagct acagcgccgc atgaattttg caaaaaagca
     8941 attttatcag acaacatggg attgtcaccg accacaacga caccactgat tacatcagaa
     9001 tgtccgttca gatatttggt tgctgaatga agtacaatgt caaatccgag ctcaagaggg
     9061 cgttgaatcc agggggttgc aaaggtgtta tcggcaactg taatcaaatt atgttttttt
     9121 gcaatagcag cgatttttcg taaattagct agctttagca tgggattgga aggggtttcc
     9181 aaccatagta attttgtctt cggagttatt gcggcttcaa tattctcggg tactgacata
     9241 tcaataaaag aaaaggataa attagaagtt cgggttttta ctttatcaaa aagccggaaa
     9301 gttcctccat aaagatcatc catggcaaga acgtgatctc cggaatctaa taaatcaata
     9361 actgtattga ttgcagccat gcccgaagca aaagcaaatc ctttttggcc tgattcaagg
     9421 cttgctatgc aatcctcata ggcttttctg gtggggttct gggtgcgtga gtattcatag
     9481 ccaagatgtt cgccaggggc aatttgctta taggtggagg tagcataaat aggggtcatc
     9541 acggcccctg tactttgaca tggttcttgt cctgcatgaa ttgcacgtgt atcgaaatga
     9601 gtcttattca ttttttattc ccataaaaat atttaaattc aaagaaatac caataggcga
     9661 tcaaaataat gattaaaaat tatacttttg gcgccttttt tgctaaagtt catagtgtaa
     9721 atgccgatat ttttatagag tcattctaga gagggatgca tgcgtaggca agaaatcaat
     9781 tatgaaaaag ttgccaaggc cgcagaaaaa ataaaaaaaa gaggcataga gccgtccgtt
     9841 aatgaaattc gtgacgaact tggcttagtt gggaaccatc cgcaattatc aattttgctg
     9901 gaggagtggt atcacaatca accagaattt aaacgaaaaa gtcacacacc tttgactgaa
     9961 aatattaatt taaataccga tgaaattcgc gaaaaaaatg tagagcttga aaaatcaatt
    10021 tcgctgttac gggcaacact cgaatccact gcagatggta ttatgatggt taatggccat
    10081 ggagccgttg tcgattggaa tcaaaagttt gtagaaatgt ggcgaattcc ctcttatatg
    10141 atggagtcgg gaaaagaaag tatcagtttt gaatacatac ttgagcaact tattgatccg
    10201 caatctttga ttgctgatgt gcaatatctt tatcaaaatc tagagtggca aggtgaatta
    10261 cctgagcttc atttcaaaga cggtcggatt tatgagcgtt ttactcagcc tcagcgcgtg
    10321 ggctcacaaa tagtggggcg agtatatagt ttccgtgatg ttactcaaaa gaggatggct
    10381 ctcgatgagt tgagaatacg tgaacgtgct attgaagcca gcacacatgg tgtggtgatt
    10441 attgacgtaa caaaaaatga aaacaaagtc atttatgtca atcgagcttt cgaaagaata
    10501 acgggatatg gggagcaaca tgctttaggg aagggtttgc ttactttgct aggttcaaat
    10561 ttggaggagg taaaccataa acgtattgaa ctggcaatta gggaaagcaa agaagaaaca
    10621 attgaaatgg aaagtataaa acgaaatgga gagttctatt ggtgtgaaat cagcgttgct
    10681 ccggttaaag attcttttgg ctatgtaaaa cattatattt gcattttaaa tgatgtgact
    10741 caacgacgtg atatggaaga tcaactgttg cagcaagcaa cttatgattc cctgactaat
    10801 ttacctaacc gtgtcttact gatggataga gtggagcagg ccattctaca agcaagaaag
    10861 aacaaggcaa ttttggcttt tttatttttg gatttagatc ggtttaaact gactaatgat
    10921 actttgggac acagtatggg ggataaatta ttacaagcta ttgccaatcg attactcatt
    10981 gttacagagg attttgatac tgttgccaga ttggggggcg atgagtttgt gatattactt
    11041 acagacatag ataatatgct ggaagccgaa acaattgcac aaaatatttt aaaaattata
    11101 gaaaaaccta ttcaaataga tcaacatagt ttgaaaatta caggtagttt gggaatcagc
    11161 ttttatcccc gagatggtga tgattacgaa tctttgatga aaagtgctga tttgtctatg
    11221 tatcatgcga aagatactgg aagaaacaac tatcgtgtct acgaacctga aatgaatagg
    11281 cgagtcatta atcatatgca attagacaat gcgttaaggg atgctttaaa aaatgatgaa
    11341 ttatttctgg tttatcaacc attgattgac ttaaagcaat ccagagtagt cggattcgag
    11401 gctttaatga gatggcatag caaaatattg ggactggtta ttccggcgga ttttataccc
    11461 atggcagaag aaaatggcat gattttggaa atgggtgaat gggcaatgaa gcaagcttgc
    11521 atacaagtaa aagaatggca taaagctggc tttaaaaact taagtattgc ggtgaatctt
    11581 tctggacggc agtttcgaca aaaaaacttg ccggaggttg tatcgcgagt attaaaaagc
    11641 tctggtttgc aatcgagatt tttggaactc gaattaactg aaagcttgtt gatagaagat
    11701 attgatcacg ttgtagatac tatgtatgca ttgaaagaca tgggaaccaa gttagttatt
    11761 gatgattttg gtacaggtta ctcaagcttg tcttatttaa aacaatttcc cgtagataaa
    11821 ctgaaaattg atcgttcgtt tatcactgaa atggtaagca atcagaatga tgcggctatt
    11881 gctaaagcga ttattaacct gggccacagt ttaaatcttc aggttcttgc ggagggcgtt
    11941 gaaaatgaat ttcaaagaga ctttataaca tctcatggat gtgactatgc tcaaggatat
    12001 ttttttaagg ctcctgatac tccagaaaat atattagaat ttttaaaaag tttatctgaa
    12061 tcgaaaataa aataataact aattcattaa gttgagaatt cctgatttca ataaaaactc
    12121 aaatcccatc ataaatctct ccagatcggt atgttttttg caagcaaaga ttaaattaat
    12181 caagtagttt ctgttggctt tgtttatttt atctttcatg ttgtttatat gaagggcaag
    12241 tatatagaac aactcaaaac gacaggttga gcctgagttt tacttttatt caagcaatat
    12301 attgattgaa tttgtgtatt ttttaaggag attcggaata gatgtggtgt tgatatagaa
    12361 tgggttggga ggataaatga agcacattac cataattgga gccgggttag cgggaacttt
    12421 gtgtggttta tatcttgcta gaagaggcta tgaagtagag ttgtttgaat caagaccaga
    12481 cattcgtaat agtccgactg attatggacg ctccattaat cttgctttgt catgcagagg
    12541 aattaccgct ttaaaagcaa tgaacctgtt aagtgaagta aataaaatta tggttccaat
    12601 gcgggcaaga gctattcacg aagccaatgg cgaggttcac tatcaaccat ttggacgtca
    12661 cattgatgaa tatatcaatg ctatttctcg ttctgatctc aatgccttgc ttttaaataa
    12721 agcggaatta tgccctaaca ttaaattaca ctttaatatg aaattgcact ctcttgatat
    12781 tcataataaa aaaattaaat ttgaaaataa aaatggtgat tttgttgagg cctcatatca
    12841 tagactaatt ggagcagatg gtgcgccgtc tcatgtaagg gatatgctga aaaatgaagg
    12901 gatagttagt gcaagcagag attttttatc tcatggatac aaggagcttt caattagcaa
    12961 aaaacataca aaagggatgg ctagagagca tttgcattta tggccacgtg attcgtttat
    13021 gctattaggt aaccctaatc ctgacgattc aattacaggt tctttgtttt tagctaatga
    13081 agggaaagac agttttgctg aactaaataa cgaagaaaaa ttacatttat tttttaaaac
    13141 acagttcccg gatgcctatg cagctatgcc aaatttggtt caagaatttt ttggtaatcc
    13201 tactggacat ttgagtacta tccaatgctc accctggtac tataaagacg aatgcttatt
    13261 aataggtgat gcggcacatg ggataattcc attctttggg caaggaatga atagcgcatt
    13321 tgaagattgc cgtattcttg atgaattact ggatgaatac caagacgatt ggtcgcgggt
    13381 gacccctgtt ttttatgaac aaagaaaagt gaataccgat gcgatagcga aaatgtccat
    13441 ggataattat catgagattc attcggacat aagaaatcca aaatttattt tacaaaagca
    13501 gattgaacgg gaactcatgt tgcgataccc ggaacattat gtttccatgc atgttttggt
    13561 tatgtttacc aataccccgt atgctaaagc gatggcaata ggagagttac aatctggcct
    13621 tttagagcaa atttgtttcc caataactga tattaaggag ttaaattggc aggaagtaga
    13681 gaaattactt agcatatatg acaaaaaatt ggcgaaaatt atttgattta atttatgtca
    13741 ttcattttaa atgaaattta tagtcaaagt tttgtcagaa aagatggatt cagggacatt
    13801 tttttgtcgc tacctgaggg ttattagcgc aaagaataaa aaaataactt aatttttgtt
    13861 atattattga gcattttgac caaaaagatg gcacgccttc tgcagtataa acttgtctaa
    13921 ataaagttta tatggagtct gttatgaaaa ctgttcaagc ctatattgat tcaaaacaac
    13981 atgagttcat gaatcatcct ttcttcaaca ttttagaaga attaaatagt atagaagaaa
    14041 tcagttattt tgtcccggag ttaacatttt gggcaatgac ttttcaagat atacttcgaa
    14101 ttaatgaaga aagagtaact gatccttatt taaagaaagt agctcgacat cacagattag
    14161 aggatgcagg acacgaaaaa tggtttttaa gtgataaaaa atacatgggt gaaatatgtg
    14221 ataacaaggc ctgtgatcgc gatgatgttg cctggctata cagtaaggat tcccaattgg
    14281 tacgtgatgc tgcctattct attatgtctg aaatttataa aatagaggat gaaaatctga
    14341 atattgtctt gctgttgacc ctggaatcat caggacatgt attttttgag aaagttgtca
    14401 aacaagttaa aaagacgggt gaagataaaa atctgaaata cttctcaagt tctcatcttg
    14461 aagtagaatt agcacatgct ttatttgaag aggaaatgga aagaaaagta tattccagaa
    14521 gattaccaat tagcactcgt agagaagcac taaggatggt agatcgttgc tatgatgcat
    14581 tcagcaagat gtttgatgga ttgattcttg cttgcaataa acgattggaa ttggcaaaag
    14641 aaaggaatca acatgtcgca aaatcagtgg agcttagaga aaatcaagca gtataaggaa
    14701 gataccggac aagcattgct cagtgatgag caatcgcttg ttttatttgg tcaggatttt
    14761 ggcaaactca ttcaatcaca gccggtagct gtgtctgccc ctcaaaatat agagtcattg
    14821 caatcattaa ttttattcgc caatcaatat cacttaccag tgactattcg tggtaatggt
    14881 ttaagtcaag gtggccaatc attacctgtt cctggaggac taacaatttc tatgcagtct
    14941 ttcaacaagc cacttgattt gagtgaagat ctcatctggg ttgaggctaa taccagttgg
    15001 aagaatctgc ttgaaaaatc actactgaaa aataaagccc cctatgtgct gccttataac
    15061 tgtaatttgt cggttggggg tgttttatct gcaggaggaa taggagcttc ctcttttaaa
    15121 tacggtgtta ttaatgctta tgtttcagca ttggaagtgg ttgatggttt ggggagaaag
    15181 caaatcgttg aaaaaaactc acctctattt caagcctgcc tttcagggca aggtcgattt
    15241 ggtgtcatta ccaaagcatg tattcagcta cgatcagttc agccacgcgt caaaactttc
    15301 tttctggttt atgctgatca gaatcaatgg tttgaagata tttataaaat acagggtaaa
    15361 gtcgattata tggagatatt ttgttctcct tcaattcagg gcgctcgatt gaaagaagat
    15421 aaacgtattc ctatggctta ttggttgtat ggtttgcacc tttctgttga atatgatcaa
    15481 catgtagaag atattttagg gcagttaaag ccatggaatg tcctgaacat acaggaagaa
    15541 agcattttat cttatttttt aaggcatgac tctcgctttg acatgatgaa attgacaggg
    15601 caatgggatt tgttacatcc ttggtatgaa tgttttgtcc ccacctcatt tttaaaagga
    15661 atactttccc aactattaga agaattacca cttcattatg ccagtttggt tcatgtagtt
    15721 ccaattgccc agaaaaaagc cgggtttatg atgctgccag atagcgattc aatttgtgag
    15781 ttcatgattt taaatccagg agtaccttct tctttggcgg aaagttgcct gcaggcgatt
    15841 gagagtctcg ataagtactt gctacaaaat aatggtaaac gctatttatc aggtttctta
    15901 ggtacagaat taccagacaa ttattggtta aagcattttg gagaaaaata cgattcttgg
    15961 gcaggtttaa aaaaacagta tgatccagca ggtatttttt catcggtatt acatcaaata
    16021 taaagagtat tgtgcaaacc catgccttgt aatagatact aatgcgtggt ggcagatctg
    16081 atggataaag gtatgaattc agcctggata aagatatatt atctacagga cttacgaaaa
    16141 cccccaatct ctttgttgaa caatgttttt aattcggtca tttagtttat ctaaactcct
    16201 tcattaaaaa cattttttgc tttgaccttg gggtttttcg taagccctga tctagtgatc
    16261 ttgaagtgag gatgataaag atctattttg tggtaggatg ctatgtatcg atattattta
    16321 aagagaagga tgaatttgtt ttgataggtt taactgggat tggaaacgta tgaaatttga
    16381 tattcaattt ttgttttcag ccaattacgc taaatggata atcattagtt tagtctccct
    16441 gtttggtatt ttgattattt atgaatggtc caccttattt tatccattga cttctgttaa
    16501 ggctatcccg ggttcaaata caaagtctat tgctggaatg aaagaagaga atctaaatta
    16561 tcttttcaat acatccttat ttggagttta tgttcctgca gatttgaatg aggataatgt
    16621 aaaacaatcg atgttgaatg ttactttggt cggtattctt tttgcagaca agatagaaga
    16681 gtctcaagtg attatacgtt ctgccagtgg ggaagaaaaa acctattacg taggagataa
    16741 aattccaggt ggtgcaacaa ttaaacgtat tatgccagga ggagtgcttg ttgaaagaga
    16801 tggcacttta gaaagtttaa gtcttccgaa aaatgattta acctttgaac ctgtcgctaa
    16861 gcctttaaaa gaggaataaa tggcatgcaa cgcatcttta agttattcat tctaataatt
    16921 tttattccca tgatggctgc ctgtataact ggtaccttaa atttacgcga agggatccaa
    16981 tgctttaaag ctcaggatta caggaaagca ttcattcgct tgaaacccga agctcaaaag
    17041 ggaaatcctg atgctcagta tgctgtaggt tatatgtatt attatggtaa gggtgtggta
    17101 gaagatagaa aaaaagcccg tttctggata acctgcgctg caagagcagg tcaaccagat
    17161 gccatagttg ctcttggaat tcttgatcgc caagccagac aagacagaag actatttgaa
    17221 gataataact tgcaacattt tcccgttgat aatggtttgt aagttttttt actttaatct
    17281 aatttccttt attaatgtac tttcttaaaa aacgtaaccg ttgacgccat tgtgtgaaca
    17341 gttactaaaa aacatgggta aaatccggga gaggttggtt tttgctaacc gtgcgcgtta
    17401 ataatcaaag aggccatgta acaaaaatac acaagtaaaa gtatcccccc aggccaggaa
    17461 gatgtctttt tttggtaata ataatttaaa aataataaaa gtaaggtaat ggaaagcatg
    17521 accggcatgt cacgccataa tactacgctg ctaactttag ttgggttcaa tagtcccggg
    17581 agagccaaaa ctaacaacat gttataaata tttgatccaa gtattgtacc aagcgctata
    17641 gaatcctctc ctttgacagc ggcaacaaga gccgtagtga gttctggcaa ggaggtacct
    17701 atggcaacaa tgatcaatcc aatggtgaat tcacttatac cagcccattt tgcaatttca
    17761 gatgcattat aaactatgta ttttgaacaa ataggcagaa tcaataagcc tatagcgata
    17821 ctcaataaat tagccggggt tgaacgacag gatagtatac ttg
//
LOCUS       DATNSN010000055        17797 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.055, whole genome shotgun sequence.
ACCESSION   DATNSN010000055 DATNSN010000000
VERSION     DATNSN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17797
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.055"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            29..685
                     /gene="adk"
                     /locus_tag="VAH76_001518"
     CDS             29..685
                     /gene="adk"
                     /locus_tag="VAH76_001518"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO1386149.1"
                     /translation="MRLMLLGGPGAGKGTQASLLINRYKIPQISTGDMLRAAIAKGTP
                     LGLSAQKIMESGGLVSDDIIIGLVKERLKNPDCDRGFLFDGFPRTLVQAEALKDAEIH
                     LDHVIEIAVDDEEIIERISGRRIHQPSGRVYHVVNQPPKNPGVDDITGEPLIQRDDDK
                     EETIRKRLQVYHSQTAPLVQYYKEWAESGSKEAPKFHTISGTGTVEQIFDNIVTILET
                     "
     gene            694..1056
                     /locus_tag="VAH76_001519"
     CDS             694..1056
                     /locus_tag="VAH76_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO1386150.1"
                     /translation="MPTQTINSAEFESLINDNEIVFIDFWAEWCAPCKQFSKVYEQVS
                     DQFPAIKFVKVNIEEQQELADFFEIRSIPHLMVFKDSIVIYSDAGSIPESTLKELAQQ
                     ALDADVSEIRAQLEQKDK"
     gene            complement(1016..2536)
                     /gene="glpD"
                     /locus_tag="VAH76_001520"
     CDS             complement(1016..2536)
                     /gene="glpD"
                     /locus_tag="VAH76_001520"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEO1386151.1"
                     /translation="MDQVFDVAIIGGGINGCGCAADAALRGLSVILFEQDDLASKTSS
                     SSTKLIHGGLRYLEHYEFGLVKKALQERQTLLNLAPHLVHSQSFVLPYLKHMRPSWLL
                     RLGLFFYDHLSRKNHLPKSRSIHRNNKNNYFTPLKEELNRGFLFYDATTDDARLTILN
                     AIQAKNHGASIRPHAKVTRAEVINNIWHLTIQPTKGQSYKTYAKSIINATGPWVQSIA
                     QLTHTPIQKDITLVKGSHIIVPKLFEGNHAYFLQHNDQRVIFVIPYHGFSMIGTTDIH
                     YTGDLNHIEIGEEEIDYLIELVNAYFKFKITKEDVVHSWSGIRPLLADDSKEVKSLSR
                     DYSYEFNHSPAPIITIFGGKITTYRQLAEEVVDQLTPVFPQMKPSKTQHTPLPGATLA
                     DMSFTQYVNYAKTKYHWLDDELLNRYLNTYGCYTEIFLSQCNNMEAMGKKYGPSLYQV
                     EVDYLILEEWANYCDDILNRRTKLGLTMDNASKKELADYLASISTYPSAQAELVFH"
     gene            2598..4073
                     /gene="glpK"
                     /locus_tag="VAH76_001521"
     CDS             2598..4073
                     /gene="glpK"
                     /locus_tag="VAH76_001521"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO1386152.1"
                     /translation="MNYILAIDQGTSSTRAMIYTLQGDLIANSQYPLTQYYPNLGWVE
                     HDPEEIWQKTLNAMRDVVSQVQGKAILCCGLTNQRETTLIWNKKTGECLAPAIVWQDR
                     RTEEFCYSLSEYSSMIQEKTGLLPDPYFSASKLNWLLKNNAQAQSLINQGDLAFGTID
                     SFLIWRMTKGASHATDITNASRTMLFNIHDEKWDKQLLELFSISESVLPEVYSSDNHF
                     GVIDKEWLGVEIPITGVAGDQQAALIGQGCFNGGMVKATFGTGGFLLLNTGDKPVFSK
                     HKLLTTIAYKIKEKTAYGLEGSIYHAGTTVKWLRDEMKLISHSAETEVLAQSLDSNNG
                     VYLVSSFTGLGAPHWLSITGTAIIGVSRSTNRAHFARAALEGVCYQTRDVLTCMRDDS
                     QLDLTLLRADGGMAVNQWFLQFMADQCQLTIQKPKDIETTAKGAAVLAAIGSGLFDSV
                     SSVNKVWEVENEFQPSREREVVEKDFRGWLEALAMVKARHA"
     gene            complement(4168..5439)
                     /gene="gltA"
                     /locus_tag="VAH76_001522"
     CDS             complement(4168..5439)
                     /gene="gltA"
                     /locus_tag="VAH76_001522"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEO1386153.1"
                     /translation="MTQKTAKLSIDGQETIDLPIYTPTLGNDVIDITQLGSHGVFTFD
                     QGFLSTASCESKITYIDGDKGILLYRGYPIEQLAEKKDFLDVSYLLLNGELPNKEEKK
                     AFVSLINNHTMVHQQMYQFLNGFRRDAHPMAIMVGIVGALSAFYHESMDLNNAQDRFI
                     SAIRLVAKMPTLAAMSYKYSIGMPYMYPKNKMSYAENFLHMMFGVPSEDTTPDPVLVR
                     AMDTIFILHADHEQNASTSTVRLAGSTGANPFACISAGIGALWGPAHGGANEACLNML
                     KEIGDVSRIDHYIKRAKDKNDPFRLMGFGHRVYKSYDPRAKVMRKTCYDVLEAVGAKD
                     APLFKLAMELERIALEDEYFIEKKLYPNVDFYSGLTLSAIGIPTNMFTVIFALARTVG
                     WMSHWMEMVATKSRIGRPRQLYTGEKIRDVK"
     gene            5665..6546
                     /locus_tag="VAH76_001523"
     CDS             5665..6546
                     /locus_tag="VAH76_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEO1386154.1"
                     /translation="MITPAELPINHRGAVYHLDLRTEEIADLIITVGDPGRVSLVSQH
                     FDKIEVQRSHREFVTHTGYLGTNRITVLSTGIGMPNIDIVMNELDALANIDLSTRTVN
                     TNKKSLTIIRLGTTGGVTASCLPGDIAISRYAIGFDSLIDYYQYQPMGVLETIRDELS
                     NYLKGESGPFYISESCPHLAEQFSALGHTGITATCGGFYGPQGRQLRMPLHYPNLISQ
                     LGKFCTQNYSVINLEMETAAIYALGSMLGHKCLSLSVVINNRIHGSFVTDVKSCLDSL
                     ITKSLANIKNILEPYEA"
     gene            6630..9221
                     /gene="gyrA"
                     /locus_tag="VAH76_001524"
     CDS             6630..9221
                     /gene="gyrA"
                     /locus_tag="VAH76_001524"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO1386155.1"
                     /translation="MVYLAKEVLPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYLL
                     IDGQGNFGSVDGDAPAAMRYTEVRMSKVAHALLADLDKETVDFSPNYDETEFAPVVLP
                     SRIPNLLVNGSSGIAVGMATNIPPHNLTEVINACIALVDEPDTSLEDLMEIIPGPDFP
                     TAAIINGRAGIIEGYRTGKGRVVIRARTEIETDENSGRQSIIIQELPYQVNKARLIER
                     IAELVRDKKIEGISGLRDESDKQGMRVVIELKRNEVADVVLNNLFAHTQMQNVFGINM
                     VALVDGQPRTLNLKQILEYFIKHRREVVTRRTIFELKKARNRAHLLEGLGIALANIDE
                     MIALIKQSPTPQDAKNALLSKIWQPGLVKAMLEKAGSNASRPDDLTEEYGLHENGYKL
                     SEAQAQAILELRLHRLTALEQDKIINEFEELLNLIKELLDILASPERLMQVIRDELIE
                     IKSQFGDERRTEITASQEDLTIEDLITEEDVVVTLSHQGYVKYQPITAYQAQRRGGKG
                     KSATHVKDEDFVERLVIASTHDTLLCFSNHGKLYWLKAYQLPQASRTSRGRPIINILP
                     LAEGEEINAMLPVREYKDGSYVFMATKKGTVKKVPLNAFSRPRSNGIIAVDLEEDDSL
                     VGVDITDGARDIMLFTDAGKVIRFDESKVRPMGRTARGVRGIRVEKDQAVKSLVVVDP
                     SGGTILTATENGYGKRTHIDEYRVSGRGGQGVISIQVTERNGKVVRSLQVTDNDEAML
                     ITDKGTLVRFKVNELSVIGRNTQGVRLINVSSGETVVGMQKIVDLGEELEESEDSSLN
                     ADDNSDE"
     gene            9214..10302
                     /gene="serC"
                     /locus_tag="VAH76_001525"
     CDS             9214..10302
                     /gene="serC"
                     /locus_tag="VAH76_001525"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO1386156.1"
                     /translation="MNSRVFNFGAGPAMLPEEILKEAQEEFLNWRNTGMSILEIGHRT
                     PEIINLLSTAEQSLRELLNIPKNYHVLFLGGAARAQFAMIPMNLLQPGDEAAYFITGI
                     WSKMAYHEANLLKQAYYLSNEEKEGFVSIPDYQKWELKSNTAYVYYTPNETINGVRFP
                     YVPKTGGVPLVADMTSCLLSEPININQYGLIFAGAQKNIANAGLTVVIIHEDLLKNQP
                     EPVIPTMLNYKNHAEHRSLYATPPVFNCYLASKMFEWIKTQGGIEGLFQRNCLKAAKL
                     YQYLDSTDFYLTPVSKEARSIMNICFSLCYPDLEQKFLYMANERGLKALKGHRFAGGL
                     RASLYNAMPMAGVDALIEFLSEFAKENG"
     gene            10308..11609
                     /gene="aroA"
                     /locus_tag="VAH76_001526"
     CDS             10308..11609
                     /gene="aroA"
                     /locus_tag="VAH76_001526"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO1386157.1"
                     /translation="MLNFISKPVVCLKGEITVPGDKSISHRSIIFGAIAIGTSVIDGF
                     LDGEDCIATLKAFQSMGVRIEGPDKQRVIIHGVGKYGLKQPQNIIDCGNSGTSMRLLA
                     GLLAAQQFDSQLTGDESLLKRPMLRISRPLSQMGADVTTQDGKPPILIKGGKKLNGIH
                     YVMPEASAQVKSCLLLAGMYAEGQTKITENAVSRDHTERMLRTFSYPVQIQDGVIVID
                     RNGECHGTRLNIPGDISSAAFFIVAASITPGSDILIRNVGINPTRTGIIHILTEMGAD
                     IKVLNQRAYGEEPVADLHIRYSQLKGIDIPVSMVPLAIDEFPVIFIAAACAQGKTTLH
                     GAKELRLKESDRIGAMVDGLNQLGVHAEGFDDGILIEGGSIQGGEVNSRGDHRIAMSF
                     AIAGAVASAPVTIKNCANVATSFPSFVTTANILHFQIEEYS"
     gene            11609..12301
                     /gene="cmk"
                     /locus_tag="VAH76_001527"
     CDS             11609..12301
                     /gene="cmk"
                     /locus_tag="VAH76_001527"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO1386158.1"
                     /translation="MVFNNEVPVITLDGPSGTGKGTICHLVAKRLHWNMLDSGAIYRV
                     LAYAARKNSIEPNDIKKLTALARSLNLRFESSADNETKVILDDENVSQQIRSEQCGQD
                     ASQIAVIPEVRAALLERQRNFAQFPGLVTDGRDMGTVVFPNAVLKVYLYASAEERANR
                     RYLQLQDNGINVSLAQVVEELAKRDARDTARTHAPLKPAEDAVLIDTTGLTIVQVFNI
                     VLKLIDERLNNL"
     gene            12367..14043
                     /gene="rpsA"
                     /locus_tag="VAH76_001528"
     CDS             12367..14043
                     /gene="rpsA"
                     /locus_tag="VAH76_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO1386159.1"
                     /translation="MSESFKELFEQSIAGAQFYPGAIITAKVIDIDDDFVTLNAGLKS
                     EGIVAVEEFYDKNGELEVKVGDTVEVALDSVEDGHGETLLSREKAKRQEAWRKLSKCH
                     ENNETVTGLISGKVKGGFTVEIGSIRAFLPGSLVDVRPVRDPSYLEGKELEFKVIKMD
                     LKRNNIVVSRRAVVEEESSADRQALLESLHDGQELHGIVKNLTDYGAFIDLGGIDGLL
                     HITDISWKRVKHPSEVLSVGQDVKVKVLSFDSERNRVSLGMKQLGNDPWVDLVERYPI
                     GKRLQGKVTNITDYGCFVEIEEGVEGLVHMSEMDWTNKNVHPSKVVSLGDVVDVMVLE
                     IDEERRRISLGMKQCVGNPWQQFASTHNKGEKVKGKIRSITDFGIFIGLDGDIDGLVH
                     LSDISWTVPGEEAVKQFKKGQDLEAVILAIDPERERISLGLKQLEGDSFASFAETYTK
                     GSIVKGTVTAVEPKTVTVALAEDVSGTIRVSELSDERVDDATTIVKVGDEVEAKITNI
                     DRKNRTISLSVKAKDAQDEADAIKKYSRTEAASTTLGDLLKEKMASKEGE"
     gene            14157..14447
                     /locus_tag="VAH76_001529"
     CDS             14157..14447
                     /locus_tag="VAH76_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEO1386160.1"
                     /translation="MRILMLVTYLLLIIIGVSFAALNASSVQVNFYFKTVSMPISVLM
                     TIMLGVGVFIGFVLFITRYWRLKIECRKMKNQLKLTEKEIKNLRSIPLQDQH"
     gene            14494..15663
                     /gene="lapB"
                     /locus_tag="VAH76_001530"
     CDS             14494..15663
                     /gene="lapB"
                     /locus_tag="VAH76_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEO1386161.1"
                     /translation="MINLWPLLLPAAAWSGWWLASRSNSNKDAHVHNRLSREYVVGLN
                     YLLNEQSDKAVDVFIKLLEVDSDTVETHLALGSLFRRRGEVDRAIRIHQNLIARPQLS
                     IQQRKEALMALGQDYMSAGVFDRAERIFLEVVELGGSRDTSSLQGLLAIYQQEKAWEK
                     ALDIIKKLEISTGASFHNQAAHYYCEMASQALKINAMDRAIYCIKQAMNVDPESVRAS
                     LMNATIEIKEGRYKQAIRSLKRVPQQDAEFLTEIIEPLVLCHKELDSMPDCIHYLEQT
                     LEKHPRASVIFVIADYLRQNKGMDIAIDFVADNLSKHPSIRGLNRLIYWHLESAHGKV
                     REKLQMLYDITSKFLDNKPIYRCGHCGFGGKHLHWHCPSCKQWGRMKPIHGLEGD"
     gene            15922..17037
                     /locus_tag="VAH76_001531"
     CDS             15922..17037
                     /locus_tag="VAH76_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEO1386162.1"
                     /translation="MQFIDLKTQYSLIENDILNSIKKVLNHGQYIMGPEIAELEKELA
                     TFVGVKHAIVNSSGTDALLMALMALDIQPGDEVITSPFSFFATAEVISLCHATPVFVD
                     IDPLTYNLDPEQLERAITKKTKAIMPVDLYGQCADYDAINAIAAQYGIPVIEDAAQSF
                     GATYKGKYSCSLATIGCTSFFPSKPLGGYGDSGACFTNDDVLAQKLIEIRIHGQNARY
                     CHHRVGINGRMDTIQAAILLQKLKIFSNEILMRQKVAKRYDQMLSELVKTPYVHECNA
                     SVYAQYTIEVENRDVFAKSMQASGIPTAVHYPIAMHQQQALGYLSYKLGDFPNSEKAS
                     QHVISLPMHPYLQEDEQLKIVAAVRNALLVMNEEAMV"
     gene            17038..17727
                     /gene="pyrF"
                     /locus_tag="VAH76_001532"
     CDS             17038..17727
                     /gene="pyrF"
                     /locus_tag="VAH76_001532"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO1386163.1"
                     /translation="MTPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQ
                     FVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESL
                     KTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
                     VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNP
                     HEVVSALLRDL"
BASE COUNT         5494 a         3029 c         3907 g         5367 t
ORIGIN      
        1 tgattatgaa ttatttaagg agttttggat gcgattaatg cttttaggtg gaccgggagc
       61 cggcaaagga acccaagctt cacttttaat taaccgttat aaaattcctc aaatttctac
      121 cggtgacatg ctgcgtgctg ctatagcaaa aggtacgcca ttaggattaa gtgcccaaaa
      181 aatcatggaa tcaggtgggt tggtgtctga tgatattatt atcgggttag tgaaggaaag
      241 actaaaaaac cctgattgtg atagaggatt cctgtttgat ggttttccaa ggacattggt
      301 ccaggccgaa gctttaaaag atgcagaaat ccatttggat catgtgatag aaatagccgt
      361 cgatgacgaa gaaataattg aacgcatcag cgggcgacgt attcaccagc cttctggtag
      421 ggtttatcac gttgtgaatc aaccgccgaa aaatccgggt gtagatgaca tcacaggtga
      481 gcctctgatt caacgtgatg atgataaaga agaaaccata cgtaagcgct tgcaggttta
      541 ccatagtcaa acagcgccat tggtgcaata ttataaggaa tgggctgaat ctggctctaa
      601 agaagctcca aaatttcaca ctattagtgg tacaggtaca gtagagcaaa tatttgacaa
      661 catcgtaacg atactcgaaa cttaggagtt aacatgccga cccagactat taacagcgct
      721 gaatttgaat cattaattaa tgacaatgag atagttttta ttgatttctg ggctgagtgg
      781 tgtgctccct gtaagcaatt ttcaaaagta tatgagcaag tttccgatca atttccagct
      841 attaaatttg ttaaagttaa cattgaagag caacaagagt tagcagattt tttcgagatc
      901 cgatccatcc ctcatcttat ggttttcaaa gacagtattg ttatttattc tgatgctgga
      961 agcatacccg agtccacttt aaaagaattg gcgcaacaag cacttgatgc tgatgttagt
     1021 gaaatacgag ctcagcttga gcagaaggat aagtagatat actagccaga taatccgcaa
     1081 gttctttttt actggcatta tccatagtaa ggccgagctt agtgcggcgg ttaagtatgt
     1141 catcacaata atttgcccac tcttctaaaa taagataatc tacttccacc tggtagagtg
     1201 atgggccata ttttttcccc attgcttcca tattgttgca ttgcgaaaga aaaatttcag
     1261 tataacaacc ataggtattg aggtagcgat ttaatagttc atcatccagc caatgatatt
     1321 ttgttttggc gtaattgaca tattgagtaa aactcatatc cgctaaagtt gcccccggta
     1381 agggcgtgtg ttgtgttttc gatggcttca tttgtggaaa tacaggggta agttgatcta
     1441 caacttcttc agccaactgt ctgtaagtgg ttatttttcc tccaaaaatt gtaataatag
     1501 gagctgggga gtggttaaac tcataggaat agtcacgact taatgatttg acttccttgc
     1561 tatcatcagc taataaaggc cttatgccgc tccaggaatg gactacatct tctttagtta
     1621 ttttaaactt gaagtaggca tttaccaatt caattaagta atcaatttct tcttcaccaa
     1681 tctctatgtg gttaagatcc ccagtatagt ggatatctgt ggttccaatc atactaaagc
     1741 catgataggg gataacaaaa ataacccgct gatcattatg ttgcaagaaa taggcatgat
     1801 ttccctcaaa aagtttgggg acgataatat ggctaccttt aaccagggta atatcttttt
     1861 ggataggtgt atgagttaat tgggcaatgg attgcaccca tggtcctgtg gcattaatga
     1921 tgcttttagc ataagtttta tagctctgcc ctttggtggg ctggatagtt aaatgccata
     1981 tgttattgat tacctcagca cgggttactt tggcatgtgg ccttatgctt gcgccatgat
     2041 ttttggcttg tatggcgtta agaatggtta agcgagcatc atccgtggtg gcatcataga
     2101 aaagaaaacc gcgatttagc tcctctttta gtggcgtaaa ataattattc ttattatttc
     2161 tgtggatcga tctggatttg ggtaaatgat tttttcgact gagatgatcg taaaaaaata
     2221 aacctaacct caataaccat gaggggcgca tatgttttaa gtagggcaaa acaaaagatt
     2281 gggaatggac taaatgggga gccaaattga gaagcgtttg acgttcctgg agtgcttttt
     2341 tgactaaacc aaattcataa tgctctaaat accttaaacc accatgtatt aattttgtgc
     2401 tacttgatga ggttttcgaa gccagatcgt cctgttcgaa gagtatcaca gacagacctc
     2461 ttaatgctgc atctgcagcg cagccgcagc cattgatgcc tccaccaata attgcaacat
     2521 caaaaacctg atccatgttt ttatctccga aaaaagatta cactcaatat atataaatat
     2581 agagggattt tataataatg aactatatac tagctattga ccagggaaca agtagtactc
     2641 gcgctatgat ctacaccctt caaggtgatt taattgcaaa tagtcagtat ccgcttactc
     2701 aatattaccc caatttgggt tgggtagaac acgatcctga ggaaatatgg caaaaaactc
     2761 tgaatgcgat gagggatgtc gtatctcaag ttcaaggaaa agcaatttta tgctgtggcc
     2821 ttaccaacca acgggaaact actctgattt ggaacaaaaa aacaggggaa tgcctcgcgc
     2881 cagctattgt gtggcaagat agacgtacgg aagagttttg ttattcattg tctgaatact
     2941 cgtcaatgat acaagagaaa accggattac ttcctgatcc ctatttttca gcgagcaagt
     3001 taaactggtt actaaagaat aatgctcaag ctcaatcact aattaatcag ggggatttgg
     3061 catttgggac aatagacagt tttttaatat ggcgcatgac taaaggtgcc agtcatgcta
     3121 ccgatatcac taacgcatcc agaactatgc tgtttaatat tcatgatgaa aaatgggata
     3181 agcagttgct ggagcttttt tctatttcag aatcggtatt accagaggtt tactccagtg
     3241 ataatcattt tggggtgata gataaagaat ggctgggagt agaaatacca attaccgggg
     3301 ttgcaggtga tcaacaagcc gctttaattg ggcaaggttg ttttaatggc ggaatggtta
     3361 aagctacttt tggtacgggg ggatttttat tgctcaatac aggtgataaa ccagttttct
     3421 caaaacataa attgttgact actattgcct ataaaattaa agaaaaaaca gcttatggtt
     3481 tggagggtag tatttatcat gccggaacaa ctgttaaatg gttacgcgat gaaatgaagt
     3541 tgatatctca ttctgctgaa actgaagtat tggcacaaag tcttgatagc aataacggtg
     3601 tatatcttgt ttcatctttt actggtcttg gagcacctca ttggttatca ataacaggaa
     3661 cggctattat tggcgtatcc cgttcaacga atagagcgca ttttgccaga gccgccctgg
     3721 aaggcgtttg ttatcaaaca cgtgatgtat taacctgtat gagagatgat agtcaattgg
     3781 atttgacttt attaagagcg gatggaggga tggcagtaaa ccaatggttt ctacaattta
     3841 tggctgatca gtgtcagttg acaattcaaa aacccaagga cattgaaacg actgccaaag
     3901 gggcagcagt attagccgca ataggttctg gtctatttga ttcagtttct tcagtaaata
     3961 aggtatggga agttgagaac gagtttcagc catccaggga aagagaggta gttgaaaaag
     4021 attttcgtgg ctggttggag gcgttggcaa tggttaaagc aaggcatgca taggctagtc
     4081 ttatatttaa attagtgcga ttaaattaga tcgcagtagc tcgtattata gtttaataca
     4141 agctactgat taacacgttg gattttttta ttttacgtca cgaatttttt caccagtata
     4201 caattggcga ggtctgccaa tgcgtgactt ggtggctacc atttccatcc agtgactcat
     4261 ccatccaaca gtgcgggcta gagcaaatat cactgtgaac atattggttg gtattccaat
     4321 agcgcttaaa gtcaatcctg agtaaaagtc tacattcggg tagagttttt tctcaatgaa
     4381 atattcatct tcaagagcaa tacgttcaag ttccattgct aatttaaata aaggtgcgtc
     4441 ttttgctcca acggcttcta acacatcata acaggtctta cgcattactt ttgctcttgg
     4501 atcatagctt ttatagaccc tgtgcccaaa acccatcaaa cggaatggat catttttatc
     4561 tttggctcgt ttaatgtaat gatcgatgcg actcacatcg cctatttctt ttagcatatt
     4621 taagcaggct tcattcgctc caccatgagc tggtccccat aaagccccta taccagctga
     4681 aatacacgca aaggggttag ctccagtaga gcctgccaga cgtactgtcg atgtagatgc
     4741 attttgttca tgatcagcat gtagaataaa aatagtatcc atagctctta ccaatactgg
     4801 atctggcgta gtatcttcgg atggaacacc aaacatcatg tgaagaaaat tttctgcata
     4861 agacatttta tttttaggat acatgtaggg catgccaatg gagtatttat aactcattgc
     4921 cgctaaagta ggcattttag caaccagtct gattgcggaa ataaaacggt cttgtgcgtt
     4981 attcaaatcc atggattcat gataaaaagc agacaaagcc cccacaattc caaccattat
     5041 agccattggg tgagcgtcac gtctgaatcc attcaaaaat tggtacattt gctgatgaac
     5101 catagtgtga ttgttgatca gactaacgaa agctttcttt tcttctttgt taggtagttc
     5161 tccattgagt aataaataac ttacatcaag gaagtccttt ttttctgcca attgttctat
     5221 aggatacccc ctatacaaca aaatgccttt atccccatca atataggtga tttttgattc
     5281 acaggaggca gttgataaaa aaccttgatc aaaggtaaag acgccatgtg aaccaagctg
     5341 ggtaatatca atcacatcat tacctagggt gggagtatag attggtaaat caatcgtctc
     5401 ttgcccatcg atgctgagtt tcgctgtctt ttgagtcatt gcttctcctg attatttaca
     5461 tgctttaagc attcataatt gcgttagtgc gcggaactat ataaaaatga caacatacag
     5521 tcaaattaaa cgtaattttg gtcattaaaa atgctagccc aatatccaat gttaattaaa
     5581 cattcaaata atttagatca tttgcaaccg tatacattga ttatatatta tggtttacaa
     5641 atttgaggaa taatagaaaa aaaaatgata actccagctg aattacctat taatcatcgc
     5701 ggtgcggtat atcatcttga tttaagaaca gaagaaatag cagatctcat cattacagtt
     5761 ggagaccctg ggcgagtaag cctagttagt caacattttg ataaaattga agtgcaacgc
     5821 tcccaccgag aatttgttac tcatacaggg tatctcggaa ctaatagaat tacagttctc
     5881 tcaactggca ttggcatgcc aaacatagat attgtgatga atgaactgga tgctttggca
     5941 aacatcgatt tatctacacg aacggtgaat actaacaaaa agagtttaac aattattcga
     6001 ttaggcacca caggaggagt gactgcgagc tgtctgcctg gagatatagc gattagtcga
     6061 tatgcaattg gttttgatag tttgattgat tattatcagt atcagccgat gggagtgctt
     6121 gaaacaatta gggacgagtt atctaattat ctaaagggag agagtggccc attttatatt
     6181 tctgaatcct gcccgcactt agctgaacag tttagtgcct taggccatac gggaattaca
     6241 gccacttgtg gtggttttta tgggccgcaa ggccgccagt taagaatgcc tcttcattac
     6301 cccaatctta ttagtcagtt aggaaaattt tgtactcaga attattcagt aattaatctt
     6361 gagatggaaa ctgctgccat ctacgcactt ggtagtatgc ttggacataa atgcttatcc
     6421 ttaagtgtgg tgattaataa caggatacat ggaagttttg taactgatgt caaatcttgt
     6481 ctggattccc taattacaaa atcacttgcg aatattaaaa atatccttga gccatatgaa
     6541 gcttaaggtc atttaattaa gacgcccagt gtggtatcct tttgcattaa attaatctta
     6601 atttattgtc taaaggatac atccaaatca tggtatacct agccaaagaa gtcttaccag
     6661 tcaacataga agacgaattg aagcaatcct atttggatta tgcgatgagt gtcattgtag
     6721 gccgagcatt gcctgatgta cgtgatggtt taaaaccggt gcataggcga gttctttttg
     6781 cgatgagcga gttgggtaat gattggaata aaccttataa aaaatctgct cgtgtagtag
     6841 gggatgtcat cggtaaatac catcctcacg gggatacagc tgtttatgac actattgtcc
     6901 gtatggccca acctttttcc atgcgctacc ttttaatcga tggacagggt aattttggct
     6961 ctgtagatgg agatgctcca gctgctatgc gttacactga agtaagaatg tccaaagtgg
     7021 cacatgcttt actggctgac ttggataagg aaaccgttga ttttagtcct aactatgatg
     7081 aaacagaatt tgctccagtg gtattgccat cgagaattcc caatttgcta gttaatggct
     7141 cttccggtat tgcggtaggg atggctacta atattccacc acataatctt actgaagtaa
     7201 tcaatgcatg tattgcctta gtggatgaac ctgacacgag tcttgaagat ttaatggaaa
     7261 ttattcctgg ccctgatttt cctacagcag caattattaa tggtcgtgct ggaattattg
     7321 aaggttatcg tactggaaaa gggcgggttg ttatcagggc acgcacagaa attgaaacgg
     7381 atgaaaattc aggccgtcag tcaattatta ttcaggaatt accttatcag gtgaataaag
     7441 cgcgtttgat cgagcgtatc gctgaattgg taagggacaa gaaaatcgag ggaatttccg
     7501 gcttgaggga tgagtcagac aagcagggaa tgagagtagt cattgaatta aaacgcaatg
     7561 aagtagcaga tgtggtattg aataacctgt tcgctcatac tcaaatgcaa aatgtattcg
     7621 ggattaatat ggttgccctg gtagatggcc aaccgcgtac tttgaatttg aagcaaatac
     7681 tggaatattt tataaaacat cgaagagagg ttgttaccag gcgcacaata tttgagttga
     7741 aaaaagccag aaatcgagcc catttattgg agggcttggg aatcgcctta gctaatatcg
     7801 atgaaatgat cgcgttaatt aagcaatctc ctactcctca agacgcaaaa aatgctttat
     7861 taagcaaaat atggcaacct ggcttggtaa aggctatgtt ggaaaaggct ggctcaaacg
     7921 cgtcaaggcc tgatgattta actgaagagt atggtttgca tgagaacgga tataaattat
     7981 ctgaagcaca ggcacaagca atacttgaat taagattaca taggctaaca gctcttgaac
     8041 aagataaaat aattaacgaa tttgaagagc tattgaattt aattaaagaa ttgcttgata
     8101 tattggcttc acctgaaaga cttatgcaag ttattcgtga tgaattgata gagattaaat
     8161 cccaatttgg tgatgagaga cgaacggaaa taacagcctc acaagaagat ttgactattg
     8221 aagatttgat taccgaagaa gacgttgtgg tcactttatc tcatcaaggt tatgtaaaat
     8281 atcagcccat tacagcctat caggctcagc gccgaggtgg caaaggcaaa tcagcaactc
     8341 atgtcaagga tgaggatttt gttgagcgtt tggttattgc gagtactcat gacaccttat
     8401 tatgtttctc caatcatggc aaattatatt ggctaaaagc gtatcaattg cctcaagcaa
     8461 gccgcacttc gagaggaagg cctataatta atattcttcc tttggcagaa ggtgaagaga
     8521 ttaatgctat gctccctgtt cgtgagtata aagacggcag ctatgtgttt atggcaacca
     8581 agaagggaac agttaaaaaa gtacctttaa acgcatttag caggccgcgt tccaatggta
     8641 ttattgctgt ggacctggaa gaagatgaca gtctcgttgg agtcgatatt actgatggtg
     8701 ctcgcgatat tatgttgttt accgatgcag gcaaagtaat ccgctttgat gaaagtaaag
     8761 tgcgacctat gggacgcacg gctcgaggag ttcgcggaat tagggtagaa aaggatcaag
     8821 ccgttaaatc ccttgtggta gtcgacccaa gtggaggtac cattttgacg gcaactgaaa
     8881 acggctacgg caagagaact catattgatg aataccgagt gtcaggaagg ggggggcaag
     8941 gcgttatttc cattcaggtt actgaacgaa atggtaaagt ggtccgctcc ctacaggtta
     9001 cagataatga cgaagccatg ttaattacag ataaaggaac tttggttcgt tttaaagtta
     9061 atgaattatc tgtgataggc agaaatacac aaggagttcg cctgattaat gtcagttctg
     9121 gtgagacagt tgttggaatg caaaaaattg tagatcttgg agaagagtta gaagaatcag
     9181 aagattcatc ccttaatgct gatgacaaca gtgatgaata gcagggtttt caattttgga
     9241 gctggccctg ctatgctgcc cgaggaaata ttaaaagagg ctcaagaaga attccttaat
     9301 tggcgtaata ctggaatgtc catacttgaa atagggcata gaacaccgga gatcattaat
     9361 cttttaagta ctgcggagca atcgttaaga gagctgttaa atatccctaa aaattatcat
     9421 gtattgtttt taggaggagc agctcgggca caatttgcca tgatccctat gaatttatta
     9481 cagccagggg atgaggcggc ttattttatt actggaatct ggtctaagat ggcttatcat
     9541 gaagcgaatc ttctaaaaca agcatattat cttagcaatg aagagaaaga gggatttgta
     9601 tcaattcctg attatcaaaa gtgggagtta aaaagcaata cagcttatgt ctattatacc
     9661 ccaaatgaaa caattaatgg agtcagattt ccttatgttc ctaaaacagg aggggttcct
     9721 ttagttgcgg atatgacttc ttgcttgctg agcgagccga taaatattaa tcaatatggt
     9781 ttgatttttg ctggtgcgca gaagaacatt gccaatgcag gtttaactgt agttatcatt
     9841 catgaggatt tactaaaaaa tcaacctgaa ccagtgattc ctaccatgtt gaattataaa
     9901 aatcatgctg aacatcgctc cttatatgct acaccaccag tatttaattg ttatttggca
     9961 agtaaaatgt tcgaatggat aaaaactcag ggaggtatag aaggattatt tcaaaggaat
    10021 tgtttgaaag ccgcgaagtt atatcaatat cttgattcaa ctgattttta tctaactcca
    10081 gtatctaaag aagcccgttc tattatgaat atttgttttt ccctctgcta tcctgatttg
    10141 gaacaaaaat tcctctatat ggctaatgag cgtgggttaa aggccttaaa aggtcatcgt
    10201 ttcgcaggag gattgcgcgc cagtttgtac aatgccatgc ctatggctgg agttgatgcg
    10261 ctcatagagt ttttgtctga atttgcaaaa gaaaatggtt aataagtatg ttgaatttta
    10321 taagtaaacc agttgtctgc cttaagggcg aaatcacagt acctggagat aaatccattt
    10381 cccatcgttc tattattttt ggcgcgattg ctattggaac ttcagtaatt gatggctttt
    10441 tagatggtga agattgtata gctaccctta aagcattcca gtcgatgggt gttaggatag
    10501 aggggcctga taagcaacgt gtcattattc atggggttgg aaaatatggt ttaaaacaac
    10561 ctcagaatat tattgattgt ggcaactcag gtaccagtat gcgcttgctg gctggattgc
    10621 ttgcagcaca acaatttgat agccagttaa cgggagacga aagtttactt aaacgcccaa
    10681 tgttaagaat cagtagaccc ttaagtcaaa tgggtgcaga cgttactacc caggatggga
    10741 agccgcctat attaatcaaa ggcggaaaaa aattaaatgg aatacattat gttatgccgg
    10801 aagccagtgc tcaggtaaag tcgtgcctgt tgttagctgg tatgtatgct gaaggacaaa
    10861 caaaaataac tgaaaatgca gtcagtcggg atcataccga aagaatgtta agaacatttt
    10921 cttatccagt tcagatacaa gatggtgtta tcgtaattga tcgtaatggc gagtgtcatg
    10981 gaactcgtct gaatatcccg ggagacattt catctgctgc tttttttatt gttgcagctt
    11041 ctattactcc aggatccgat attttaattc gtaatgtagg tattaatcct acacgaactg
    11101 gcattattca tattttaact gaaatgggag cggacataaa agttttgaat caacgtgcct
    11161 atggagagga accagttgct gatttgcata tcagatattc ccaattaaaa ggaatcgata
    11221 ttcctgtctc tatggtgcct ttggcgattg atgaattccc ggttattttt attgcagctg
    11281 cttgcgcaca agggaaaacc actcttcatg gagctaaaga gctgcgcttg aaggaaagtg
    11341 atcgtattgg tgcgatggtg gatggcttaa accaattggg agttcatgct gaaggttttg
    11401 atgatggaat attgatagag ggtggaagta tacagggtgg tgaggttaat agtcgaggag
    11461 atcaccgtat tgccatgtca tttgccattg ctggcgctgt tgccagtgct cctgtaacga
    11521 ttaaaaactg cgccaatgtt gcaacttcat ttccatcttt tgttactacc gctaacatac
    11581 ttcattttca gatagaggaa tacagctgat ggtttttaat aatgaggttc ctgtcattac
    11641 gttggatggt ccaagtggaa caggaaaggg gacaatttgt catttggtag ccaagagact
    11701 gcattggaat atgcttgaca gtggcgctat ttatcgtgtt ctagcttatg ctgctcggaa
    11761 aaatagcatc gaaccgaatg atattaaaaa attgactgct ttggcccgtt ccctgaattt
    11821 gcgttttgaa tcatctgcgg ataatgaaac aaaagtaata cttgatgatg aaaatgtcag
    11881 ccagcaaatc cgcagtgagc aatgtggtca ggatgcctca caaattgcag tgattcctga
    11941 agtaagagca gcactgcttg aacgccagag aaactttgcc caatttcctg gattggtgac
    12001 agatggacgc gatatgggta cggtggtctt tccaaatgct gttttgaagg tttatttata
    12061 tgcaagtgca gaagaaagag caaatagacg atatttacag ttgcaagata atggaattaa
    12121 tgttagcctc gcccaagttg ttgaagaact ggctaaacgg gatgcaaggg atacagctcg
    12181 tacgcatgcg ccattgaaac cagctgaaga tgctgtcttg atcgatacaa ccggactaac
    12241 aattgtacaa gtgttcaata ttgtattaaa attaattgat gaacgtttga ataatttgta
    12301 gttttgaatt ggggggaagg tgagtggaac tcattcttcc atttttttac taaagagttt
    12361 attaacatgt ctgaaagttt caaagaatta tttgagcaaa gtattgccgg agctcaattt
    12421 tatcctggtg ccattattac tgctaaagtc atcgatatcg atgatgattt tgttactctc
    12481 aatgctggac taaagtcaga aggtattgtt gccgtagaag aattctacga taaaaatggc
    12541 gaattggaag ttaaagtagg cgatactgtc gaagtggcct tggattcagt agaagatggt
    12601 cacggcgaaa cgctcctttc aagagaaaaa gcaaaacgcc aggaagcatg gcgcaagtta
    12661 tcgaaatgcc acgaaaataa tgaaacagtc actggtttga tttctggaaa agtcaaaggt
    12721 ggttttactg tggaaattgg ctcaatcaga gcatttttac cgggttccct tgttgatgtt
    12781 aggcctgtaa gagatccatc ttacctcgaa ggtaaagaac ttgaatttaa agtcatcaag
    12841 atggatttga aacgcaataa tattgttgtt tctcgacgag ctgtagttga agaagaaagt
    12901 agcgctgaca gacaagcact actggagtct ttacatgatg gtcaagagct tcacggtatt
    12961 gttaaaaacc ttaccgacta tggtgctttc attgatcttg gcggcataga tggtttgtta
    13021 catatcactg acatttcctg gaagcgagtc aagcatccaa gtgaagtatt atcagtaggt
    13081 caggatgtta aagtcaaagt actaagcttt gatagtgaaa gaaaccgcgt ttctttgggt
    13141 atgaagcagt taggaaatga tccttgggtt gatttggttg aacgttaccc aataggcaag
    13201 agattgcaag gtaaagtaac taacatcacc gattatggtt gctttgtcga aattgaggaa
    13261 ggtgtagaag gtttagtcca catgtcagaa atggattgga ctaataaaaa tgttcaccca
    13321 agcaaagtag tgtctctggg ggatgtagtc gacgttatgg ttcttgaaat tgatgaagaa
    13381 cgacgacgta tttctttagg tatgaaacag tgtgtgggca atccatggca gcaattcgca
    13441 tctactcata ataaaggcga aaaagtaaaa ggcaagatcc gttctattac tgactttgga
    13501 atctttatag gtcttgatgg tgatattgat ggactagttc atttatctga tatttcctgg
    13561 actgttcctg gtgaagaagc ggtaaaacaa tttaaaaagg gtcaagattt agaagctgtt
    13621 attttagcaa tcgatccaga gagagagcgt atttctttag gattgaaaca attggaaggc
    13681 gacagttttg ccagctttgc tgaaacttac acgaaaggat ctattgttaa aggtacagtt
    13741 acagctgtag aacctaaaac tgtaacagtt gcgttagcag aagatgtttc tggcacaatt
    13801 cgtgtgagtg aattatccga tgaacgtgtt gatgatgcta ccactatcgt aaaagttggt
    13861 gatgaagttg aagcaaaaat cactaatatt gatagaaaaa atcgcaccat ttcactttct
    13921 gtcaaggcaa aagatgccca ggatgaagcg gatgcaatta agaagtactc cagaacagag
    13981 gcagcatcaa ctactctggg tgatttattg aaagaaaaaa tggctagcaa agaaggtgaa
    14041 taaccttttt aagtcaaaac taaaaaagcg tagaactatc tacgcttttt ttttattttg
    14101 agaaaaagat tatggtatga tggacgtttt agcttaagga ttaaaggaaa tattcaatgc
    14161 gcatattaat gctggtaact tatttactgc ttattattat tggtgttagt tttgcggctc
    14221 taaatgcctc gtcggttcaa gttaatttct attttaaaac ggtgtccatg cctatctctg
    14281 ttcttatgac tatcatgctc ggagttggtg tttttatagg ttttgttctc tttattacac
    14341 gatattggcg tttaaaaatt gaatgtcgta aaatgaaaaa ccaattaaaa ctaactgaaa
    14401 aagaaattaa gaatttacgt tcaataccct tacaagatca acattaattt tattattagt
    14461 ggaaactcga tacgcagaga ggaaaattaa tagatgatta atttatggcc actgttgtta
    14521 cctgctgccg catggtctgg ctggtggctg gctagccgca gtaattccaa taaagatgct
    14581 catgtccaca atcgcttatc gcgagaatat gtagttggac ttaattattt attaaatgaa
    14641 caatctgata aagcagttga cgtatttatc aagcttctag aagttgatag cgatacagta
    14701 gaaacccatc ttgcactggg tagcttgttc aggcgtagag gggaagtaga cagggctatt
    14761 cgtattcatc aaaatttaat cgccaggcct caattaagta ttcaacaacg aaaggaagcc
    14821 ctaatggctt tagggcaaga ttatatgagt gctggagtgt ttgaccgagc tgaacgtata
    14881 ttccttgaag ttgttgagtt gggcgggagt agagatacaa gcagtcttca aggattatta
    14941 gcaatttacc aacaagaaaa agcttgggaa aaagctttag atattattaa gaaactggaa
    15001 atttctaccg gggccagttt ccacaatcaa gcagctcatt attactgtga aatggctagt
    15061 caagcgctta aaatcaatgc aatggatagg gcaatttatt gtataaagca agcaatgaat
    15121 gttgatccgg aatccgtaag agccagtttg atgaatgcta caattgaaat aaaagaaggc
    15181 cgctataagc aggctattcg atctttaaaa agagtgccgc agcaggatgc agagttttta
    15241 accgaaatca ttgaaccatt agttctttgt cataaagagc tggactccat gccggattgt
    15301 attcactatc ttgagcaaac tttggaaaaa catccgagag cctctgtaat tttcgttata
    15361 gctgattatt tgcgacaaaa taaaggtatg gatattgcca ttgactttgt ggctgataat
    15421 ttaagcaaac atccatcgat aagaggtttg aatcgtttga tttattggca cttggagtct
    15481 gctcatggga aagtacgtga aaaactgcaa atgctttatg atattactag caagttttta
    15541 gataataagc ccatatatcg atgtgggcat tgtggttttg gtggtaaaca tttacattgg
    15601 cattgcccaa gttgtaaaca gtggggtaga atgaagccta tccatggttt ggaaggggat
    15661 taacctgaga catactctca gaaagttaat gaacagttgc atattagctt agtatcgtaa
    15721 ttcaaatttg ttattgcaaa ttaagtaaaa caattcgaac aggaaattta atttgatttg
    15781 ttttgtcttc tcgtttgcca aggcagttta cgttcaaatc agtttattgc aactttagat
    15841 ttgtattaag attattcctt aacttcatgg tattgatctt aggtatagct aaacaattta
    15901 tatactaatt atgagattat aatgcaattt atcgacttaa aaacacaata ttctttaatt
    15961 gagaatgata ttttaaacag cattaaaaag gtattaaatc atggccaata tattatgggg
    16021 cctgaaattg cagaacttga aaaagagtta gccacttttg ttggagtgaa gcatgcgatt
    16081 gtgaattcaa gtggtacaga tgctttatta atggcattga tggctctgga tattcagcct
    16141 ggggatgaag taattacaag cccatttagt ttcttcgcaa cagcagaagt gattagcctt
    16201 tgtcatgcaa cacccgtctt tgtagatata gatcctttaa cttataattt ggatcctgag
    16261 cagttagaga gagcaatcac taagaaaacc aaggccatta tgccagttga tttatatggg
    16321 caatgcgcag attacgatgc gattaatgcc attgcagctc aatacggtat tcctgttatt
    16381 gaagatgctg cgcagagttt tggagctact tataaaggca aatactcttg ttccctggct
    16441 actattggct gtaccagttt tttcccatca aaacctttag gtggttatgg tgattctgga
    16501 gcttgtttta cgaatgatga tgtattagcc caaaaattaa ttgaaattag aattcatggg
    16561 caaaatgccc gctattgcca tcatcgggta gggattaatg gtcgaatgga cactatccaa
    16621 gcagcaatac tacttcagaa actgaaaata ttttctaatg aaattctaat gcgacaaaaa
    16681 gtagcaaaac gttatgatca aatgctttct gagttagtga aaactcctta tgtgcatgaa
    16741 tgcaatgcga gtgtgtatgc tcagtatacc attgaggttg agaatagaga tgtatttgcg
    16801 aagtcaatgc aagcctcagg tatacctaca gctgttcatt acccaattgc tatgcatcaa
    16861 cagcaggcac ttggttactt aagttacaaa cttggagatt ttccaaactc tgaaaaagca
    16921 agtcaacatg taattagcct gcctatgcac ccttatttac aagaagatga gcaattgaaa
    16981 atagttgcag cagttagaaa tgctttactt gtgatgaatg aagaggccat ggtataaatg
    17041 acacctaaat taatcgttgc gttggatttt gataatcaag ataacgcttt acagttggtt
    17101 gaaaaacttg atccgaatca ttgcgcactt aaggttggga gtgagctttt tactctattg
    17161 gggcctcagt ttgtaaaaga attggtaaga agagagttta aagtattttt agatttaaaa
    17221 tttcacgata tccctaatac agttgctaaa gcatgccact ccgctgcaga attaggggta
    17281 tggatgataa atgttcatgc cattggtggc ttgaaaatgt tgcaagctgc cagggaatct
    17341 ttaaaaacgt atggtaaaga caggcctttg ttgattgcag tgactgtgct gaccagtttt
    17401 gaagaagggg agctggccag tgttggcatt agtaatacat tgcctgagca agcaacccat
    17461 ctggctatgc ttgccagaga ggctgggctt gatggtgtgg taagctctgc acatgaagtt
    17521 aaaattataa aacagaagtg tggtgagaat tttattaccg ttactcctgg cataagactg
    17581 ccaaataatc taaaagatga tcaatcgcga gtaatgactc cacaacaagc catcagagaa
    17641 ggaagtgatt ttctggttat tggaagacca attacgcaag cgagcaatcc tcatgaggtt
    17701 gtctcagctc tattgcgtga tctttgatgg tgcatacttt taaaaaatta tacatatggt
    17761 ataattttgt tcatttttga attagttgta ccattcc
//
LOCUS       DATNSN010000056        17599 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.056, whole genome shotgun sequence.
ACCESSION   DATNSN010000056 DATNSN010000000
VERSION     DATNSN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17599
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.056"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(213..1106)
                     /gene="prpB"
                     /locus_tag="VAH76_001533"
     CDS             complement(213..1106)
                     /gene="prpB"
                     /locus_tag="VAH76_001533"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEO1386164.1"
                     /translation="MSNFRYSQGKKLREALMKEKPLQVVGTINAYAALQAKRAGFHAI
                     YLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTI
                     KEMIKAGAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
                     DALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVT
                     PLFTLEELKEAGVSLALYPLSAFRAMSAAAEKVYDTIRKNGSQKDILAEMQTREELYQ
                     VLNYHFYEDKLNELFMKEKTT"
     gene            complement(1415..2149)
                     /locus_tag="VAH76_001534"
                     /pseudo
     CDS             complement(1415..2149)
                     /locus_tag="VAH76_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843025.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(2139..2843)
                     /locus_tag="VAH76_001535"
     CDS             complement(2139..2843)
                     /locus_tag="VAH76_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3865 domain-containing protein"
                     /protein_id="HEO1386165.1"
                     /translation="MIREYMDKRNNQFTTEILNYFSDLKKFSTSYLEWLTMEHYQFSL
                     NNTRFLMNSHIQTKSLSDPGVSEELLANYNEEKNHAQMYRHALKKIGLDVTNRIPYVA
                     TRQFFMRLEALIEINASKTLGVMYATETAAIFEHILFKEISAELCQRKRLDFENSRIK
                     QFHDLHLDGVEQAHKDELGKFMDHTLAQEHVDPNIFLQEKEIIDGAYAAIDIMEDWWS
                     GLINAKAHLLEQAHAQ"
     gene            2949..3857
                     /locus_tag="VAH76_001536"
     CDS             2949..3857
                     /locus_tag="VAH76_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1386166.1"
                     /translation="MINFSQIQVFIAIVESGGFIAAGEKMHRSQPAITNAIKKLESSL
                     GFELFDRKVYRPQLTAKGKAFYQKAREVAAQFMQLENSAETLRSGVETQFSIDLDIAL
                     PLPGYAPLIQSFIRQYPETCFSLSNNTFVRCLERLERRECHLCIAASHIRSPQIEYLR
                     MPSIRMIPVASRDYYDQHKERINNPKLGIYCMQIMIAGTQQELYQLKIEPLSNSMPKW
                     FVGDMYIRKQLILSGMGIGRLPDHLVEKELAEGSLVQLNTRDYVAVTMDIYAMRLNNQ
                     EHGIVSNYAWQSLKSYADDTVSPELK"
     gene            4002..4721
                     /locus_tag="VAH76_001537"
     CDS             4002..4721
                     /locus_tag="VAH76_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEO1386167.1"
                     /translation="MIDFKINSQPNDESCGPTCLHAIYQHYGLDISYEEVASQVESFL
                     SGGTIAPMLGKHALQLGFKVTLYVNNLHLYDPTWFNSDGSSNTDVLREMLMQQMRYKR
                     NQYLARGTHAILDFLTLGGAIYFRCLTAEILKEYFARKIPILTGLSSTYLYNCSREIF
                     NKEGKSRSDPIRGLPCGHFVVLCGYNQKHRLVVVADPYKKNPVSGDNYYTVSIHRLIN
                     AIMLGVVTRDGNLLIIEPKYA"
     gene            4876..5874
                     /locus_tag="VAH76_001538"
                     /pseudo
     CDS             4876..5874
                     /locus_tag="VAH76_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6559..7806
                     /locus_tag="VAH76_001539"
     CDS             6559..7806
                     /locus_tag="VAH76_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1386168.1"
                     /translation="MTRSKESKTENSSFFQNHLKLSVAKLLFSEGVGDWYKKKGYGAQ
                     DTDKDFLAPFADFLKQQKTNPEATYYNAFQNLKLTLNRVPSQLVSGDSCELEVLTCEP
                     EADSPQKTGTGKHIVYFPGANTYYQACFRDISAAAKETGATVHALNYPGIGMSTGEVV
                     EANDLTNAGIAVVLSLLNQGVKPDDIVLQGDCFGASVALEVKNEIEKQADIKVRLIMN
                     NAFKSFKAAVSDLIDRSPLIPSVSKSIVKNLLEFTGWNVAPGQKYIHSDPYQCYIQHE
                     GDQTLTTATLSSKVAKYKEEIRTGRTTSRSRDPVIDKCPEEYKPFRDFLDENCFVRVR
                     DSAKERLAAKFGVDSRGEVNAHFADLCELEMLGGQSVYEGFVNNYLVMSDKYIKAHPQ
                     KDYDPDKINFLSSNPMGYTSKSS"
     gene            complement(8112..8516)
                     /locus_tag="VAH76_001540"
     CDS             complement(8112..8516)
                     /locus_tag="VAH76_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO1386169.1"
                     /translation="MNNKKIGEFAKLCGVGIETVRYYQRQGLLHVPLLNKELGAEKIR
                     RYGDDDINRLRFILLAKKAGFTLKEIKELLEFDANNDRERVRDMAERRIEKLNLKINE
                     LTEARNRLSLLVTKCIETSSSPCPILMTFEKS"
     gene            8586..9329
                     /locus_tag="VAH76_001541"
     CDS             8586..9329
                     /locus_tag="VAH76_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEO1386170.1"
                     /translation="MNHTSIKKAVLYRMVIEHHVCPYGLKARDLLRRQGYEVEDNWLT
                     TREQVDAFKDKHNVKTTPQIFINDERIGGYDDLRHYLGKKVRDPKEKSYRPVIVLFAI
                     TALMAMAVSYASFANPFTPHVLSWFIAFSMAVLALLKLQNIESFSTMFLNYDLLAKRW
                     VPYSYIYPFAEGLAGILMIAGALNWLSIPIALFIGTVGALSVFKAVYIDKRELKCACV
                     GGDSNVPLGFISLTENLMMIGMALFMLLM"
     gene            9528..10619
                     /locus_tag="VAH76_001542"
     CDS             9528..10619
                     /locus_tag="VAH76_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386171.1"
                     /translation="MSKEIKKNLQNNNSAFVYLRSTGLATNSLSSILQYMTRIIVLTE
                     SAGIALGILGVLFSVIGFVSSTIARIFDPTASKLSRVMSAGILIAAAAGAIPLIYLGT
                     LFPAMWVYLGIGIMGFGVLNSSYYLYSDVRKENALINQYKDNPEQYVESIKEKRQAKY
                     EKLLNTRLDDKQARVLLVFKLYQLDKKLAVAKQEGLARLYDIVCHRKHTRSLERFIDL
                     GTSLTSLIVGVSVLFWGIAAWPIGPILLGISLAVFSVSLSKSAIQFYRKVNKNNALLK
                     EVLSNDRYISLLDANLDNPYDIEEMGTMEKTQEKVCTHELSQEPALSKDQEHKEHYTK
                     LFQPVNKTALRPNDEETDELSCKSVLLQR"
     gene            complement(10625..10933)
                     /locus_tag="VAH76_001543"
     CDS             complement(10625..10933)
                     /locus_tag="VAH76_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386172.1"
                     /translation="MGEVRMDLYLKTQRWLYHRGNREVKKRILDEFCKTHGYHRKAAA
                     RLLRQLPIPDKKTKKQVKRKLMTRTYYWSCLKRFGLPGIRFVAHCGVNSKGSGTLIGL
                     "
     gene            11073..11567
                     /locus_tag="VAH76_001544"
     CDS             11073..11567
                     /locus_tag="VAH76_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HEO1386173.1"
                     /translation="MKAFNVPKSMETLFSKAQAYVQDYFQNLKIDPSKGTIDINGERY
                     ILVRAASMSIEFFETVKKLYENDASKSEEVVFQLLFDISHSIGKQDAKKFHKQLSLKD
                     PIEKLSAGPPHFAFSGWAFVDILPESKPTPDENFYLVYNHSYSFESSAWLKANKKINF
                     LFAS"
     gene            11822..13363
                     /locus_tag="VAH76_001545"
     CDS             11822..13363
                     /locus_tag="VAH76_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEO1386174.1"
                     /translation="MNKLPKWALAEKKEFKREKIQSLLIVPMEKENSLVGFMGFDIVT
                     HKKQWLAEDIFMLSTVADIVIQSILKSYTIIEHESIVRYFDPLTRLPNKELFVNYLQA
                     VLSNIEQQENCFSFLILLNIDEFHIINDAYGTPFGDQLLFIIAEKLLHILPYKAKLCR
                     LEADRFLILLQGNKAEILKIADEILSMFNHVFKLNNHQVYLQARIGVTKIDNHSDNPE
                     KLIQRASLAMRKARLDNIKIAIFESNTEKSVNLKLNIQAELYQAITENQLVLHYQPQF
                     CAKTKQIVALEALLRWMHPKKGLLYPSEFLDVLLCSDLLLPAGEWVLKTVCAQNVAWI
                     KQGLRPVSIAVNISEPQFRQPNFVPMLKKILTKTHMDPCLLKLEVTEQVMIDHLDETA
                     RKLHEIEAHNIQIILDDFGTGYASLSYLHQLPISKLKIDKSFIKNMLTNKNAAMITKT
                     IILLAKGLGIKSLAEGVETKEQFDILKQLGCDEIQGYYFSEPLPVEKIALFLQDGSTP
                     NKGAF"
     gene            13545..13877
                     /locus_tag="VAH76_001546"
                     /pseudo
     CDS             13545..13877
                     /locus_tag="VAH76_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
     gene            14220..14402
                     /locus_tag="VAH76_001547"
     CDS             14220..14402
                     /locus_tag="VAH76_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386175.1"
                     /translation="MSILIKFESLAKQAIFSSNKDIILQSIGLLDTIKAKNNQKIRMT
                     LSNSSEQFADMSRVVR"
     gene            14417..15181
                     /locus_tag="VAH76_001548"
     CDS             14417..15181
                     /locus_tag="VAH76_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HEO1386176.1"
                     /translation="MKKLALVGSGIKSISHFTFEFNTYTTSADKVLYLVNEPITKQWI
                     ERYSKLSESLDPIYFAENDRQISYDKIRDKILTELETHNFITVVLYGHPTVFADPGLQ
                     SIIIAQKKSIETIVLPGISVENCLYADLKIDPGQFGCFHVEATELLLYDKIIDPTVHL
                     CIWQPGMIGNRSVPKPNQKSNHLELLRIKLKKYYPDDHISILYEASMYPGVEPIIHKF
                     PLYDIEDQNIGTLSTLYIPPLPQRKPNLDILKQITS"
     gene            15211..16389
                     /locus_tag="VAH76_001549"
     CDS             15211..16389
                     /locus_tag="VAH76_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386177.1"
                     /translation="MLSVLSYRLSLLPKLSKTCYLLLLFSFLECVAGGIAYYLSLYLG
                     TSTPLTKLQIGQVGSIVGFGALAGALCSTYITDKLNNKNIIIASFLFLGLGFFCLPQS
                     MSVLLISGCVFIIGFATSIFLTTNNTLLLQNAPQDQNSLRFIQSMKIVSENLGNSFSI
                     LLIMFFAAQYYGQIFETIGLFFFLFALNTMKYLNVKQNSLEDSSYEEKETNQQGNNKR
                     LYVTISAVFIVGIIYSQQRIAYPLFLHETFSSFTITAILFWLDPIIVSLFQIKITKIV
                     SPLKISYQLAMGGLLLGTGLFALLIVDSIGGAIMACLTFILGEMLFMPTSFVRCYESA
                     GNKRKGMVAGAWRSAYSSGLIVGPFLSGFLMEYYSYNACWILAGILGFFLFILFSVKK
                     "
     gene            complement(16390..>17599)
                     /locus_tag="VAH76_001550"
     CDS             complement(16390..>17599)
                     /locus_tag="VAH76_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386178.1"
                     /translation="AMYLVDYGYGDLVGDDDGIFLPTKTGQYVRLDSEYTPPSMLNDL
                     AYGLSNSPLGMFFNKSFEWFLNTSDEQINRTFPIYIDPPGTFFSIGHIINYGYTATHL
                     PNGVLFVTAGSKSNFMIPKIGCGIMHSRIQKNHNITKSAPRSYHEHADIFQDIVKGNR
                     DNNGWSASVLYFSENWIEKISKDSAWQEVRDYFYRISNHRSEYAVNSDYYNHVYRVTN
                     KRNNLKSNLLIYETARHLFEILLGAKLGFAPAMDDELLPLSIIQEVYSECYKLEYLPI
                     IAIPAYYNYRDPKPVYYSLQYPSLCSYSPKARNASTTLSDLIALIDVVHKYQKDFTLP
                     IRECKNTILEEVSKITSFNFYHPATEENSIIKSPEIIPYQDERFRGEKRFPINASFFK
                     GCVSIFSNSI"
BASE COUNT         5542 a         3185 c         3295 g         5577 t
ORIGIN      
        1 atctgtttca acaaatcact tatcccaaaa ctataaatag aattgctttt cttttgcaat
       61 aactttatca aattttaaat agattgcttt tgaatgcctg tctggataga gagttaatct
      121 gttgtttata aacatcaatt tcggcagata atcctctatc caggcatata aaaccttatt
      181 ttctcaaatt agttctgata attaattgat attcacgtcg ttttttcttt cataaagagt
      241 tcattaagct tgtcttcgta aaaatggtaa tttaatacct gatacaactc ttcgcgagtt
      301 tgcatctctg ccagaatatc tttttggctg ccgtttttgc ggattgtgtc gtaaactttt
      361 tctgcagcgg cactcattgc ccgaaatgca cttaaaggat aaagagccag gctaacgcct
      421 gcttctttta attcttccag cgtaaataat ggagtgactc caaattcagt gatattcgcc
      481 aataccggaa cattacattg ctcagttagc gcctgatatt cctcaagttt acgtactcct
      541 tcgaagaaaa tcatatctgc cccggcttca atataagcac taattcgctc cagcgcctta
      601 tttaatcctt cattcgccag ggcatcagtt cttgccataa taacaaaatc aggatcggtt
      661 ttggcatcca cggcggcctt aatacgatct atcatttctt ctttttcaac caatgcctta
      721 cccgggcgat gaccacatcg tttcgcttga acttgatctt caatatgtac cgctgctgcc
      781 ccagctttaa tcatttcttt gatagtccgt gcgatgctaa aggctccacc ccatcctgta
      841 tcaatatcaa ccaggagggg caaatcaacg gcactcataa tgcgccggac gtcttcgagt
      901 acatcattta atgtggtaat gcccaggtct ggtaagccgt aagaggcgtt agctactcct
      961 gctcctgaaa gataaattgc atgaaatcct gctcttttgg cttgcaaagc ggcataggca
     1021 ttaattgttc ctacaacttg caatggtttt tctttcatta aagcttcgcg tagttttttt
     1081 ccctggctgt atcgaaaatt tgacatttgg tttccttgtt ttgcctaaga tgagaattta
     1141 tcaggactta cgaaaaatcc caatctcttt gttaaaaaaa gtttttaatt cggtcattta
     1201 gtttatctaa gctccctcat taaaaacatt ttttgccttg atcttggggt ttttcgtaag
     1261 tctggtttat tttattttta cgtttttttg aatatttttt caaacttctg gaaatgcaca
     1321 gcaaaacgac atccaaagct tgaaaagcgt atagatcatg taccaaagac aagcttgttt
     1381 gcctgatatc ataacgagag atggcaaaaa tcaactacaa atcatgaata acccaaacat
     1441 ttggctcaat gtatattcca atatcctttt ctttattgat agtgacagga tttaacccgc
     1501 ctttaacaag atattgaccc gtttgaagta acggatggcc gaaccattgt tcccactgag
     1561 caatattcgc tgtgatcacg cgtgattgct ggcatggata agctattttt cctcccagtt
     1621 gtgcatgaac gcttaaccaa ggatcaaaaa tagtattatc cactcttttc cacttcaaat
     1681 aatcctctag ggaaataagt gggtagtttt ctttttggct aggtctggct ggcactatta
     1741 gtgctttata ttgagcctcc tgtgcactgg cttttaaccg attcaataag agtttgctta
     1801 aaccaagtct ttggtaagcc ggattcacag tcactgatac agcagcaaga taatttgatg
     1861 gggttaaggg tattgtagat gccagatgag acaggaccca atttaatcct tcttgtggca
     1921 aattatcgag tgattgagta agtgctactg gcaaagaatt aataacagct actacctcac
     1981 cattatagtg agatagccat tgatataaag tctaatgact atagagctta aaaagagtgt
     2041 catttttaat gcgttgataa tcaggccaaa tcttttgtcg taaatgtaga attttctcta
     2101 tccagtcctc ttgcgccttg ccagttagcc acataagttt attgagcatg agcttgttcc
     2161 aaaaggtgtg cttttgcgtt aattaaaccg gaccaccaat cttccattat atcgatcgcc
     2221 gcatatgcgc catctattat ttctttttcc tggagaaaaa tattgggatc aacgtgctct
     2281 tgagcaagcg tatgatccat aaacttgcct aactcatctt tatgtgcctg ctctactcca
     2341 tccaggtgta aatcatggaa ttgcttgatg cgagaattct caaaatccaa acgttttctt
     2401 tgacaaagct cggcgcttat ttctttaaat aaaatatgct caaatattgc agctgtttca
     2461 gtagcgtaca taacgccaag ggttttggat gcgttaatct caatcaaggc ttcaaggcgc
     2521 ataaaaaact gtctggttgc cacatatgga atcctgtttg ttacatccag gccgattttt
     2581 tttagtgcat gccgatacat ttgagcatga tttttttctt cgttatagtt agcgagtaac
     2641 tcttcagaaa ctcctggatc agaaagcgac ttggtttgga tatgagaatt cattaagaaa
     2701 cgagtattgt ttaacgaaaa ctgataatgc tccatggtta gccattcaag ataactcgtc
     2761 gaaaattttt ttaggtcact aaaataattt aaaatttcgg tagtaaattg gttgtttctc
     2821 ttatccatgt attctcttat catgaagcct cccgtcagta ttaaaatcaa ttcatcaagg
     2881 gtttaccata actataaaga tcatataatt aaaatattat tttataatta tcttgataat
     2941 ttttagttat gataaatttt tcacaaatcc aggtgtttat tgcaattgtt gaatctggtg
     3001 gatttattgc tgctggagag aagatgcatc gttcacagcc ggcaataact aatgccataa
     3061 aaaaattgga gtccagcctg ggcttcgaac tgtttgatag aaaagtatac cgacctcaac
     3121 tcactgcgaa aggtaaagct ttttatcaaa aagcaagaga agtggccgct caatttatgc
     3181 aactggaaaa tagtgctgaa acattgagat ccggagtaga aacacaattt tctatagatc
     3241 tcgatattgc tttaccattg ccaggatatg ctccgcttat tcaatctttc ataagacaat
     3301 atcccgaaac ttgtttttca ctaagtaaca atacttttgt tcgatgtctt gagcgcctgg
     3361 aaagaagaga gtgccattta tgtatagctg caagccatat tcgttctcca cagatagaat
     3421 atttgcgtat gccttccata cgaatgattc ctgttgcgag cagggactat tatgaccagc
     3481 ataaagaacg aataaataat cccaagctgg gaatatactg catgcaaatt atgatagctg
     3541 gcacgcaaca ggaattatat caactcaaga ttgagccatt aagtaattct atgcccaaat
     3601 ggttcgtggg tgatatgtat attcgtaaac aactgatttt atcaggtatg ggtattggcc
     3661 gtttaccgga tcatctggtt gaaaaggagt tggcagaggg ctctttggtt cagttaaata
     3721 ccagggatta tgtagccgtt actatggata tttatgctat gcggttaaat aatcaagagc
     3781 atggcatagt ctccaattat gcttggcaat cactaaaaag ttatgctgat gacacagtat
     3841 ctcccgaatt gaaataacca atctttatgt ttgcttgcta atttaagcgg tttggtggat
     3901 cgaatgtttg aaaaacagga agtgattcat tgacataaaa ccagggagag cctaaattta
     3961 aatcaaaaag atataaaatt aatctccagt tggatattat aatgattgat tttaaaataa
     4021 attcccaacc caatgatgag tcttgcgggc caacctgttt acatgccata taccaacatt
     4081 atggcttaga tatcagttat gaagaggtgg caagccaggt agagagtttt ttatctggtg
     4141 gaacaatagc gcccatgctt ggtaaacatg ccttgcaatt gggatttaaa gtcacgcttt
     4201 atgtcaataa tttacacttg tatgacccga catggtttaa tagcgatggt tcatcaaata
     4261 ccgacgtcct gagagaaatg ctcatgcaac aaatgaggta taagcgtaac cagtatcttg
     4321 cccgcggaac acatgcaata cttgattttc tgactttggg gggcgctatc tattttcgat
     4381 gcctgaccgc agaaatttta aaggaatatt ttgccaggaa gattcccata ttgacagggc
     4441 ttagttcaac ctatctgtat aactgcagtc gcgagatatt caacaaggaa ggcaaatcga
     4501 ggagtgatcc tattcgcggc ctaccgtgcg gacattttgt tgttctgtgc ggatacaacc
     4561 aaaagcatcg tttagtggtg gtcgctgatc cttacaaaaa gaatccggtt tcaggtgata
     4621 attattacac cgtatccatc catagattaa ttaatgcaat tatgttgggc gtagtgacgc
     4681 gtgatggtaa tttgttgatt attgagccga aatacgcgta ggcgagcagc aaattctaac
     4741 tatcccagat caagggatta tatcaccaga tttttaatat tgatttcaat ataagaggcc
     4801 atgtatgtct tttctttatt atccgctaat aattgatata gaatggatgc tggttaattt
     4861 gatgtgtcat ttgtcatgag cgtaataatt gattccttaa aaaattctga tgtgcctcat
     4921 ctctatttgt taaaagtcgg cttaacaaaa aaagaatata acaacacctc caggatgagc
     4981 cgagatgaaa aacgacaact ggtgaataac atcatagcga aagcttctca cgaagaaatt
     5041 ttaaaaatta tcaatgactt agtggatatt gaactttcaa tagaatccac tgatccaatt
     5101 cgtacaggta atcgcctcat agggcaactg ctgctgggtt atataacaaa aattgatcag
     5161 aaaaatttta tgagttttta tgatcaaaca ataaaaaacg gcaataaaac attaggggat
     5221 tatttaatcc ctgaacaagt aaaacaaatt taggctacta taaaacaaac ggctgcaaaa
     5281 tatttttctc tgaatcaaag aggcgctgat tatcaagcgt ttctaaataa agggtttaga
     5341 attttgccta ttttttacta ccagcaacaa tttcctgaaa ttactcccga gcaatataga
     5401 caaggaatca gaccacttga attgacaaga gaaccagagg aaatcaagaa cgcatttcac
     5461 aataatttgt ccgccaatgt aacaatcccg gcatttcctg aagcgaatta tctgaagacc
     5521 agattggctg aaataaagac gcatattatg gccagtgaat ggaaactagc taattattca
     5581 ttttatagtg atggtgttat gcatggtgat aaacgccttc ctcatcgagt aaaggatatt
     5641 ctggatgtaa ttgaaaaatt cgaaagcagt aaattaaacg ccaaagcggc ttatgagcaa
     5701 attgttgtga aagctaaaga ggctttggat tacccgagga gcggtcgatt ttctgaaacg
     5761 accgattttt atcaagatat atatagtcac catatcctgc gagatgatta ccaatttaat
     5821 cattctcgag aattaacaaa ttcccatggg ccttcattta atttaaatcg ctaaagataa
     5881 atttagtcat tttaaatcat caattccagt gcaagaaaac tgctttatta acttggtaga
     5941 cggcagtgca ttttgaacga ttgcccgagc atgctattca tgtaaaaaag atgaattaga
     6001 gatatacatt gcaaatgcca ctagaaaaca agagtgccct gcaagttaaa gtatagattt
     6061 gagtttcact acagcaagca agtaacctgg tgtagggggc gtagctcaat atattttctg
     6121 aaatcaagaa ctgattaatc cagtaaagca tgggtatgct gacacagcta aagaatggct
     6181 ttattcctgt tttcatcgtc atgtccagtc tgggttattg gatgggaatt ggcgagatgg
     6241 ggagtgaatt agtggtaaag gtaactccag gacacgggtt tcgccctcct cccaggttgc
     6301 taaagtcttg cagcacaata ttcaaaattt tcaaccaaat tatcattaca gctatgcttt
     6361 cattcgagcc agccttgtcc aatcagtatc aagtgcaatg catgaggagc tacattaata
     6421 gataaatttt cgttttgagt aatgacatca tgaaatgata agagatctcc ttgcagttga
     6481 gctagaattt gatttgttca aaatcgacga actatactta aattgacgtg ttctttatat
     6541 tatcaatttg agctaacaat gactagaagt aaagagtcaa agacagaaaa tagttcattc
     6601 tttcagaatc atttgaagct ctctgttgcc aaattattgt tctcagaggg agtgggagat
     6661 tggtataaaa aaaagggtta tggagctcaa gacacggata aggatttctt ggcaccattt
     6721 gctgattttc ttaagcaaca aaaaaccaat cctgaagcca cttattataa cgcattccag
     6781 aatcttaaat taacattaaa ccgagtaccc tctcaattgg tttcagggga ttcgtgtgaa
     6841 ttggaagttc tgacatgcga accagaagca gattctcctc aaaaaacggg cactggtaaa
     6901 cacattgtgt attttcctgg cgcaaatacc tactatcagg cttgctttcg agatatcagc
     6961 gctgcagcaa aagaaaccgg agcaacggta cacgctctca attacccagg catcgggatg
     7021 agtactggag aagttgtgga agctaatgat ttaaccaacg ccggtattgc agtggttcta
     7081 tctttattaa atcagggcgt caagccagat gatattgttt tgcaaggcga ttgttttggg
     7141 gcatccgttg cactggaggt aaaaaatgag attgagaagc aagcggatat aaaagtacga
     7201 ctgataatga ataatgcatt taaatcgttt aaagcagctg tttctgattt aattgacaga
     7261 tcgcctttaa ttccctcggt atcaaaatcc attgttaaaa atttgctgga atttaccgga
     7321 tggaacgttg cccctgggca aaagtatatc cattcagatc cctaccagtg ttatattcag
     7381 catgaggggg atcaaacctt aaccaccgcg actttatcct ccaaggtcgc taaatacaaa
     7441 gaggaaataa gaacagggag aaccacatcc cgctcaagag atcctgttat tgataaatgc
     7501 cctgaagaat acaaaccatt tcgtgacttt cttgatgaga attgttttgt cagagttaga
     7561 gattctgcaa aagaacggtt agctgctaaa tttggtgtgg acagccgcgg tgaggtgaat
     7621 gcccattttg ccgatctttg tgaattggaa atgctgggag ggcaatcggt ctatgaagga
     7681 tttgttaata actatttagt catgtcagat aaatacataa aagcacaccc tcaaaaggat
     7741 tatgatcctg ataaaattaa ttttctttct tctaatccta tgggctatac ctcaaaatca
     7801 agttaattca aagcaagtaa ccctcttccc atgccctgct attgttatgt cgataaggta
     7861 agctcattga taaaatccgg ttaaaggata ataccaccca aacattccac aatttgggaa
     7921 cagtattttc tcttccagtt cggtttcaac ccgatgaatt aggacggata cagcttcttt
     7981 ggtataggca tttacgtgtt ttaggtgttg atcgagtttg agtgtttgat ttgccagaaa
     8041 agtgagttgc aatcattgct gagctcaatg attggttatc aacaatttga ttgctttcta
     8101 tttttatggg tttaactttt ctcaaaggtc ataagaatgg ggcaaggcga agagctggtt
     8161 tctatacatt ttgttactaa gagtgaaagt ctgttccgag cttcggttaa ttcattaatt
     8221 tttaaattta gtttttcaat acgcctttca gccatatctc ttacgcgttc ccgatcattg
     8281 ttagcatcaa actccaatag ttcttttatt tcttttaaag taaagcctgc ttttttggct
     8341 aaaagaataa accttaaacg atttatatca tcgtcaccat aacgccttat tttttcagcc
     8401 ccaagttctt tgttaagcaa aggaacatga agcagtccct ggcgttgata ataacgcacg
     8461 gtttcaatgc caactccaca taatttagca aattcaccaa tttttttatt attcattact
     8521 tgactccgta ctatagtacg tactctatat tactatttat agaccattaa tcatagggga
     8581 tagaaatgaa ccatacttca attaaaaaag cagttttgta tcgcatggtg atagaacacc
     8641 atgtttgccc ttatggtctc aaagccagag atttgttaag gcgtcaaggc tacgaggtag
     8701 aagataattg gctaacaacc cgtgaacagg tcgatgcgtt taaggataaa cacaatgtaa
     8761 aaaccacacc tcaaattttt atcaacgatg agcgtatagg gggttatgac gatttacggc
     8821 attaccttgg aaagaaagta agagatccaa aagaaaaaag ttatcgcccc gtcatcgtgt
     8881 tgtttgcgat taccgcttta atggcgatgg cagtgagtta tgctagcttt gctaaccctt
     8941 ttacaccaca tgttttaagc tggtttatcg catttagcat ggcagttctt gctttattaa
     9001 agctccagaa tatcgaaagc ttctcaacca tgtttttaaa ctatgatctc ttggccaaac
     9061 gctgggttcc ttacagctat atttacccat ttgccgaagg tttagcaggg atattaatga
     9121 ttgcaggtgc gttaaattgg ctttccattc caattgcctt atttattgga actgtaggtg
     9181 ctctttctgt ttttaaagcc gtttatatcg acaagcgtga gcttaaatgt gcctgtgtgg
     9241 gaggtgatag taacgtacct ttaggattta tttctctcac tgaaaattta atgatgatag
     9301 gaatggcttt attcatgttg cttatgtaat tcaactagca agcgtagccc ggatgaagtg
     9361 gaacgaaatc cgggagtatg actgcgcttg catcagtgct acgctagcta tacactgtca
     9421 atatataaaa agttgatcat cgagctatcg atatccaggg ttgttaaagt tctcttaaga
     9481 caaaccttat ataatgcaca ttttttatat ttattccagt tctgccgatg agcaaagaaa
     9541 tcaagaaaaa cctacagaac aataattcag cctttgtcta cttgcgcagt acggggcttg
     9601 ctacaaactc tctgtcgagc attctgcagt acatgacacg catcattgta cttacggaaa
     9661 gcgcgggcat tgccctgggc attctgggag tgctcttttc agtcattggt tttgtaagca
     9721 gtacgattgc aagaatattt gatccaacag cctctaaatt aagtcgtgtg atgtcggctg
     9781 gtattctcat tgcagctgca gcaggggcga taccactcat ctatctggga acgttattcc
     9841 ccgcgatgtg ggtatatctt ggtataggca ttatggggtt tggcgtactt aattcgtcat
     9901 actaccttta ttcggatgtg cgtaaggaaa atgcactcat aaatcagtat aaagataacc
     9961 ctgaacaata tgtggaaagt attaaagaaa agcgacaagc caagtatgaa aaacttctca
    10021 acacgcggct cgacgataag caagcacgag tgctgctagt ttttaagctt taccaacttg
    10081 acaagaaatt agccgttgct aaacaagagg ggctagctag actttacgac atcgtatgcc
    10141 atcgcaagca cactcgcagt ctggagagat ttattgacct tggcacttct cttacaagcc
    10201 ttattgttgg ggtgtcagtc ctgttttggg gaatcgctgc ctggccaatt ggccccatat
    10261 tgctcggtat atctttggct gtattctctg taagcctatc aaagtcggcg atacagtttt
    10321 atagaaaagt taataaaaac aatgcgcttt taaaagaagt tctctctaat gatagataca
    10381 tatcacttct tgatgcgaat ctggacaacc cgtatgacat cgaagagatg ggcactatgg
    10441 aaaaaacaca agagaaggtt tgtacgcatg agctttcaca agagcctgcg ctctcgaaag
    10501 accaagaaca taaagaacat tataccaaac tcttccagcc tgtgaataaa accgctctca
    10561 ggccaaacga tgaagagaca gacgagctaa gctgcaagtc ggttctactc caaaggtagt
    10621 ggcactataa tccgattaaa gtgccactac ctttggagtt tacaccacaa tgtgccacaa
    10681 atctgatccc tggcaagcca aatcttttta agcagctcca ataatatgtt cgggtcataa
    10741 gttttctttt tacctgtttt ttagtttttt tatcggggat gggtaactgt cttaataacc
    10801 ttgctgccgc cttacgatga tagccatgtg ttttgcaaaa ttcatccaat atgcgtttct
    10861 tcacctcacg attacctcgg tgataaagcc aacgctgtgt ttttaaatat aaatccatac
    10921 gtacttcccc catttttgct ttcctccttt cggcaacagg tattttgagg caatgcgagt
    10981 aatgtttaat gttccgtaat atttaattat agttatgggc atctggtcta taattaatat
    11041 atttataggg aataaacgta cgagttgaga gaatgaaggc atttaatgtg cccaagtcaa
    11101 tggagactct tttttcaaag gcgcaagctt atgtacaaga ttattttcaa aatttgaaaa
    11161 tagatccgag caaaggaacg attgatatca atggcgaacg atatattttg gttcgcgcag
    11221 catcaatgtc catcgaattt tttgagacag ttaaaaaatt atatgaaaac gatgcctcaa
    11281 aaagtgaaga ggtggttttt caattattat ttgatatctc acattccatc ggaaaacaag
    11341 atgcaaaaaa atttcataag caactcagtt taaaagatcc aattgaaaaa ctttcagcag
    11401 gtcctcctca ttttgcattt tctggctggg cttttgttga tatcttgccc gagtccaaac
    11461 caacacccga tgaaaatttc taccttgttt ataatcatag ttactcattt gaatcaagcg
    11521 catggcttaa ggccaataaa aaaataaatt tcctgtttgc atcatgaatg ctggttattc
    11581 atcaggttgg tgtgaagaaa gttttgggct aatgaaaatt tatatgtgtc gcccctgaga
    11641 ttaagaagaa atataaaaat gagcgctgat cctcatcact tcaaatacat gcataatatg
    11701 cacgaatggt gctcaaaatc ggtcgagccc tattttaatt ttctacaaaa tataccaatt
    11761 aaccaatttc cttggttaat ttcaaaatta aaaaaaaaag aaagtagtga atgtaagtga
    11821 tatgaacaag ttgcccaaat gggcacttgc tgaaaagaaa gagtttaagc gggagaagat
    11881 tcaatcgtta ttgattgttc ccatggaaaa agaaaattct ttggttggat tcatggggtt
    11941 tgatatagtc actcataaaa aacagtggct tgcagaagat atttttatgt tgagtacagt
    12001 ggcagacatt gtcattcaat ccatattgaa atcctacacc atcatagagc atgaatcgat
    12061 tgttcgatat tttgacccac tgacaagact tccgaacaaa gaattgtttg tcaactatct
    12121 tcaagctgta ttatctaaca ttgagcaaca ggaaaattgt ttttcctttt taattttgtt
    12181 gaatattgat gaattccata ttattaatga tgcttatgga acgccttttg gtgatcaact
    12241 gttatttatt attgcagaaa agttattgca tatactgcct tacaaggcga aattatgccg
    12301 ccttgaagct gatcgttttc tcattttgct gcaggggaat aaagcagaaa tcttaaagat
    12361 tgccgatgag attttatcca tgtttaatca tgtttttaag ctaaataacc accaagttta
    12421 tttgcaagcc agaattgggg ttactaagat agacaatcat tctgataatc cagaaaaatt
    12481 aatacaacga gctagcctcg ccatgaggaa agcaaggttg gataatataa aaatcgcaat
    12541 ttttgaatct aacactgaaa aatccgtaaa tttaaaatta aatattcaag ccgaattgta
    12601 ccaagcgatc actgagaatc agttagtgtt acattatcaa ccgcaatttt gcgcaaaaac
    12661 aaagcagatt gtggctttgg aagctctact acgttggatg caccctaaaa aaggactttt
    12721 ataccctagt gaatttctcg atgtgctgct gtgttctgat ttgcttttac ccgcgggtga
    12781 gtgggtgcta aaaacagtct gtgctcaaaa cgtagcatgg ataaaacaag gacttagacc
    12841 ggttagtatc gcagtcaata tatccgagcc tcaatttagg caacctaatt ttgtccccat
    12901 gctgaaaaaa atactaacaa agacccatat ggatccctgc ttattgaaat tagaagtaac
    12961 agagcaggtg atgattgatc acttggatga aacggctagg aaattacatg agatagaagc
    13021 tcataatata caaattatat tggatgactt tggtactgga tatgctagtt taagttattt
    13081 acatcagtta cctattagta aattaaagat tgataagtca tttataaaaa acatgctaac
    13141 caacaaaaac gccgccatga ttacgaaaac tattatttta ttagcaaagg ggttaggcat
    13201 caaatctctt gctgagggcg ttgaaaccaa agagcaattt gatatcctta agcaattagg
    13261 ttgtgatgag atacaagggt attattttag cgaaccactg ccggtggaga aaattgcttt
    13321 attcttgcaa gatggctcta ccccaaataa gggggcattt taatcaagtt agtatagact
    13381 aactctttga tttgacggtc aaaaggaaga ttaaaatgcc ccagaaagat tgtatcctaa
    13441 atttaccagg atattctatt aaaaaggtgg gtggagagaa cccggttaaa cgtttatcac
    13501 aaaccagagc ctggtatcgt gttgaacgca ggcaaagttt tattaaagcc catgaacggt
    13561 ctgttgaaaa tgctttaatt atccatgttc gtgactttct cattgagctc ggccaaggtt
    13621 ttgcctttgt tggttctcaa gtgctcttga cgtttgatga tcaattacga aagcctggaa
    13681 ataaccaaac gattggaatc ttgctatgca aatcgaaaaa taaggttatg gctgaatacg
    13741 cgttgcgaaa cattagtgct cccataggtg tttcagaata taccttatct aaatcaattc
    13801 cgactgattt gaaaggcagt ttactgacaa ttgaagaaat tgaagctgag ctaaatgagt
    13861 cggtaaaaga ggattgattg gttcaatgta ttgtaaactt ataaaaaata tatggggcaa
    13921 aaggtattag tggggcaccc atattaatac tgggttaaaa cagtcccagc atgagttgtt
    13981 tatgttggaa ctttggggac accttggacg cactctattg tctcacattt aacaaagatt
    14041 aaatttcttt aattgacaaa tagaattgaa gaatatccaa acagtaattt atattgagcg
    14101 tttcttgatt atacgtaatg atatcaatct attgattttt ccgagggaac tattttatat
    14161 agttttttca ttccatatca cttatactct tttatagagg gcatataaga gtgaatatta
    14221 tgagcatctt gattaaattc gaaagcttgg ctaaacaagc aatcttttcc agtaacaaag
    14281 atatcattct tcaatcgatt ggcctgcttg atacgattaa ggctaaaaat aatcaaaaaa
    14341 taaggatgac tttatctaat agttctgaac aatttgctga tatgtctagg gtagttagat
    14401 agtaaaagga tttatcatta agaaattagc tttagttggt tcaggtatta aatctatatc
    14461 tcatttcaca tttgaattta atacttacac taccagtgca gataaggttt tgtatttagt
    14521 taacgagcct attactaaac aatggataga acgttattca aaattgtcag aatctcttga
    14581 tccgatatat tttgctgaaa atgatcgaca gatctcttat gataaaataa gggataaaat
    14641 tcttactgag ctggaaacac ataattttat tacggtggta ctttatgggc accctacagt
    14701 tttcgcagat ccaggcttac agtctataat tatagctcaa aaaaaatcga ttgaaacaat
    14761 agtcttacct ggtatttctg ttgaaaattg cctctatgca gatcttaaaa ttgatcccgg
    14821 acaattcgga tgttttcatg tagaggcaac agaattattg ctatatgata aaattatcga
    14881 tcctacggta cacctttgta tttggcagcc aggtatgatt ggcaatcgct ctgtacctaa
    14941 accaaatcaa aaaagtaatc atttagagtt actaagaatc aaattaaaaa aatattaccc
    15001 agacgaccat atatctattc tttatgaggc gtcaatgtac ccaggtgttg aaccaataat
    15061 acataaattc ccattatatg atattgaaga tcaaaacatt ggaacacttt caacattata
    15121 tatcccccct cttccccaaa ggaaacctaa tttagatatt ctgaaacaaa taacctcata
    15181 gaataagcta ctaattattt aaggaaaaat atgttatctg tactgtctta tcgattaagt
    15241 ttactgccaa aattatcaaa aacttgctat ttacttcttc ttttttcttt cttggaatgt
    15301 gtagcaggag ggattgcata ctatctatca ttatatttag gaacatcaac accattaaca
    15361 aaactacaaa ttggtcaggt tggttcaatc gttggatttg gagcattagc aggcgcgtta
    15421 tgttctactt acataacaga taaactcaat aataaaaata ttataattgc cagttttctc
    15481 tttctaggtt taggcttttt ttgtttacct caaagcatga gtgttctgct tatatcaggt
    15541 tgtgtcttca taattggttt tgcaacaagt atttttctta ccacgaataa taccctctta
    15601 ttacaaaacg ctcctcaaga tcaaaatagc cttcgcttta tacaaagtat gaaaatagtc
    15661 agtgaaaacc ttggtaatag cttttctata ctattaatta tgttttttgc agcgcaatat
    15721 tacggtcaaa tttttgaaac aattggtcta tttttcttcc tttttgcact gaataccatg
    15781 aaatatctaa atgtaaagca aaactcatta gaagactcct cttatgaaga aaaagaaaca
    15841 aatcaacaag ggaataataa acgactttat gtaactatta gtgctgtatt tatagttgga
    15901 attatctatt cccagcaacg aattgcatac ccactttttt tacatgaaac tttttcaagt
    15961 tttactatta ccgcaatact tttctggtta gatcctatta ttgtttcttt atttcagatc
    16021 aaaataacaa aaatagtatc cccattgaaa atatcttatc aattagcaat gggagggtta
    16081 ttattaggta caggcttatt cgcattattg atagtcgata gtattggagg tgcaatcatg
    16141 gcttgtttga cgtttatttt aggagaaatg ctatttatgc ctacttcctt tgtacgatgc
    16201 tatgagtcag ctggaaataa gcgtaaaggc atggttgctg gtgcatggag aagtgcttat
    16261 tcctctggtc tgattgttgg cccttttttg tcaggtttcc tcatggagta ttatagttat
    16321 aatgcctgtt ggatattagc aggaatactt ggttttttcc tatttatttt atttagcgtc
    16381 aaaaaatgac taaatactat ttgaaaaaat cgaaacgcac cctttaaaaa aagatgcgtt
    16441 aatggggaac ctcttttcac ctctgaagcg ctcatcctga taaggaataa tttccggtga
    16501 tttgataatg ctattttctt cagtagctgg atgatagaag ttaaaactgg ttatttttga
    16561 aacctcttca aggatagtat ttttacattc ccgaatcggt aaagtgaaat ccttttgata
    16621 tttatgaaca acatcaatta aagcgataag atccgaaagg gtggtcgatg cattccttgc
    16681 tttgggtgaa taactacata gagagggata ctgaagagaa taataaacag gtttaggatc
    16741 ccggtaatta taatatgcag gtattgctat aatgggcaaa tattctaact tataacattc
    16801 tgaataaact tcttgaataa tagataatgg gagtaattca tcatccattg caggcgcaaa
    16861 accaagtttt gcgcctagca gtatctcaaa aagatgtcgt gctgtttcat agattaataa
    16921 attggatttt aaattatttc ttttattggt aactctataa acgtgattat aataatcgct
    16981 atttacagca tattcagatc gatggtttga aatcctgtaa aaataatctc taacttcctg
    17041 ccatgcagag tccttactga ttttttcaat ccaattttca gaaaaatata atacagacgc
    17101 actccaacca ttattatccc tgttaccttt aactatatct tgaaatatat cagcgtgctc
    17161 gtgataagag cgaggagccg actttgtaat attatgattc ttttgtattc gcgaatgcat
    17221 aattccacac ccaattttgg gaatcataaa attggatttt gaaccggctg taacaaaaag
    17281 aaccccattt ggtaaatggg ttgctgtata accataatta ataatatgtc ctatactaaa
    17341 aaacgtacct ggaggatcaa tgtatatagg aaacgtccgg ttaatttgct catcagaggt
    17401 atttaaaaac cactcaaatg atttattaaa aaacattcct aatggactat tagacaaacc
    17461 ataagccaaa tcatttaaca tcgatggagg agtatattct gaatcaagac gaacatattg
    17521 acctgtttta gttggcaaaa atatcccatc atcatcgcca accaagtctc cataaccata
    17581 atctacgaga tacattgca
//
LOCUS       DATNSN010000057        17481 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.057, whole genome shotgun sequence.
ACCESSION   DATNSN010000057 DATNSN010000000
VERSION     DATNSN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17481
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.057"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(134..1810)
                     /locus_tag="VAH76_001551"
     CDS             complement(134..1810)
                     /locus_tag="VAH76_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2527 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386179.1"
                     /translation="MLYFVLCDLKFNQFSRYPMSYQKIELSISLSLDSPKLDESDFIL
                     LSVKYLEKSLGKKKEFSGFFEDIEKLYFNQNYKEAIEKILDFCKKNESLLSEQVVQRL
                     AEVAPRLKSNPKDNESRRLYETLYADHLESAIKQESDLSVFNELRDSYNAVKPEYAVT
                     HETEIKTLDEAKQFILSFVMLNDNVELPLKAQSERYPKKDRSREELGNTPSANPGIMK
                     PNSPNFTDNLVPVRDVPKIAINEKVVGGYSKTKPTTPFVASLSGTTYSLMVVLTDYIE
                     KHKTDKDIEKKVNQIINLWISSYIKEGYHSYSEVVDVLTEPFLQSIFDKANIKLNYGV
                     LDDTHAEFRKAQDYVFGLTIRAAMHHELQERFKNKEKLQEEVKNFESGLNKLNQDVES
                     TGRHREALNKLNEVFQDWSAGKKSYDAFKSESNQLIRDIESEEQKSNRGLGSMLKNFG
                     NYLLYLITLRFLKEDYLKPSSPVSMLVTELRGTLEQIDNFHKNSLSLKKATQPDPKNT
                     SPSVETSDVLKSAVDNDKLNKNQDSEVKKTLDSEQMEEVQRQDDKSARLQ"
     gene            1977..3527
                     /locus_tag="VAH76_001552"
     CDS             1977..3527
                     /locus_tag="VAH76_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO1386180.1"
                     /translation="MSPRICCYNQYPGLYQDFDAMREDLQRIQKMGFTQVWVNPFYTP
                     CQYNPIPNMANRIHSPYAMQNESIYPRYAKNEKSVQQYTAKANELGLMPVFDLVARHL
                     AVDHRFVNGDKFFLEKYNIDTKKWFKRHPNGNLVMHNMDENYNPLTNNPWSDVATFNY
                     DDPVICEQIIEYYWKPFIERNIEHLGFQGIRIDAPAMVNNKVLSRLTEIAKEACQRKF
                     QRDPLIIAETIGRGYMEENLALKGIVTHTMNSVFWMPGPEGGYGYSYNLWQQDDNWFT
                     QNKGLLQQVGPTIGFPGSHDEPRYIQQLVEKGIHDDKLLAKRMREKLAVSAFCSDGGW
                     ILQYGDEYGATKPVNVFDPTPVEYHQQNHLRRFDLSDYISEINTTLAQLPNPHFPEWA
                     QRVFLPNHPDLVTFIVHQGWGYTGTSFVIATNTDPSQQIQLTEKELGEIMLANGRNNT
                     TEKQMKPQVLYLCGAVRASPELKKEIQIVASRPTVSEKSPHFFQSHPEEKKTGEKLHS
                     DNTPPQFK"
     gene            complement(3801..5531)
                     /gene="lem27"
                     /locus_tag="VAH76_001553"
     CDS             complement(3801..5531)
                     /gene="lem27"
                     /locus_tag="VAH76_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem27"
                     /protein_id="HEO1386181.1"
                     /translation="MVRVLNLQKGVNMFNVDNSGKGNCLYYAYSISLMYYLRAKNNVR
                     ITEDIFNKLGLKEEDRAHLRKLLSKDPDRAFTRDEIKTIIEPILGRATRDLAAEHTKV
                     EFKSSPHDTPLFSSLHYAVEFGFKRSLQINESELTLLIDNDFSNPDYTEAEIYKVSGL
                     LDALQEYILTRTPSVIEEFNRQWENKKQELKEQSLTEKESQVHQATILDNILRKETID
                     FLLADNEKHLDEYREHLRREFVWGSEETLMVLHRAIQGERMVRNHEGRIELVYDHEII
                     LHVHRNGASPSYQAGSPEMILNNEGNVHWTSVIPDAIFTSKLTDKEEKLLEMLERMQS
                     EYGNKELGVEKYSSISDWLSDLMKQIELIKTSPTAVTKQKEIELFFQLLAKATPKLAS
                     EPALRMSLGTLFSNFLECIPALMVENKLSTGLAKFGLYSPERSPVISQKKEDLSLIKS
                     SEEKVNSDQSQKGVLSQFSKHTLLKVSEDVSPKPYPTELNPYIRKNKQSGPRVARALA
                     QMYQKGLEGDERYTPEKCKNIAQRYIEYSKSHKRNFILDDVILFVQEMDKIIKQKETI
                     SEVTCKLVAM"
     gene            6298..7341
                     /locus_tag="VAH76_001554"
     CDS             6298..7341
                     /locus_tag="VAH76_001554"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO1386182.1"
                     /translation="MSYSILKKLPPIEKIIRELPLSHSAYLQIAQDRQEIKAILEGRD
                     KRLLMIVGPCSAWPKKAVLEYAERLVQLNSKVKHALKLIMRVYIQKPRTTKGWTGPVN
                     QPDLFSTPDIETGIKYTRDMMIKVIQMGLPIADEALFTHNAKGFLELLSWVAIGARSS
                     EDQEHRIFASALDCAVGLKNPTHGSLAVGVNSLVASQHPHVAVFDGYEVQTHGNHHAH
                     LVLRGSNQAPNYSVSHLEEVMYYMGMHQIVNPSVIIDVSHDNCLVNGKKNHQLQPEIA
                     FNILESIKNRPDLKQLVKGFMVESFIKEGNQKLDSINPDNIDLSGLSVTDPCLSWEKT
                     ESFLLELAEREAL"
     gene            7338..7922
                     /locus_tag="VAH76_001555"
     CDS             7338..7922
                     /locus_tag="VAH76_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO1386183.1"
                     /translation="MNNLVEKKEAFRFGVSGDIGSFSEEAALLYARKKGFNAHLVHLL
                     DMEGVLNAIENETIELGILPVVNLLGGLVKPAFQAMGKYSFTPIDEIWHDINQCLLVK
                     TNTQNHKITHIVSHPQGFAQCKKFLKNKFKNAKKIEWIDTAKAAKDLAEGILPPHAAV
                     IASARCAPLYDLKISARNIQDSHLNRTAFILVKK"
     gene            7957..8217
                     /locus_tag="VAH76_001556"
     CDS             7957..8217
                     /locus_tag="VAH76_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO1386184.1"
                     /translation="MSTIEELRKQIEQTDADIIEKLAQRQELSKQIGALKAKEGKKII
                     DRLREKKLFEYYDFLSRQYHLQQDFVNQLFKIIISNSKKAQK"
     gene            8217..9383
                     /locus_tag="VAH76_001557"
     CDS             8217..9383
                     /locus_tag="VAH76_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO1386185.1"
                     /translation="MNHYEKMSLLKDSATVAINSLAQQKINAGIKIHNLSAGEPKLPP
                     HPSIINAVTQAMEQGHTLYPPIMGIPELRHMGCEWMYRSYDCSFQNEQCLVVNGGKLG
                     IYLLMQLLLQPNDEVIIPSPYWVSYPAMVSLFGGTPVSVETTEAEEWKLTPKALQKAC
                     TTKSKILILNNATNPTGALYTQSELIHLLQIAKKHNLLVISDEVYSELTYDGHHYVSC
                     GSLPQFRERVIIIQSCSKNFSMTGWRVGFIFAPEIFIKPLSALVSQSTSGVTTLSQWA
                     AVAALQEVNQVNIWVQQNMEKRRNCLLRALHDYLGLTITPPVSSLYVFLSLKDIGCKN
                     QNSEEFCKQALDKANVAMVPGIAFGKEGYVRLSFGGNEDDLKSGIYALAQWLHR"
     gene            9695..10591
                     /gene="tehB"
                     /locus_tag="VAH76_001558"
     CDS             9695..10591
                     /gene="tehB"
                     /locus_tag="VAH76_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO1386186.1"
                     /translation="MPREYDNLICYKQLSINDHGKLKFFLEKHSTKEGTWGKLSLNEG
                     EIDFIFQNGLGQELSRIRLDKNNRQLSIPPASWHKIIPISASFDAQLGFYCKPHRYFN
                     KKYGLTEVHRDLLYVYHTYLSHLGSLTTLDVGCGSGRNLLYLAQLGHTVTGIDINQTA
                     LENIQIIAQKENLSPLNILSQDLNQPQSLAPDHYDFVCSTVTLQFLNASRIPSLLIEL
                     QEATKKNGYHFLVFPVQSELYSLPSSFTFLPKQEELYHFYQDSGWSILEYKESVGHLH
                     KQDELGRPVQGLFALLLAQKVI"
     gene            complement(10624..11055)
                     /locus_tag="VAH76_001559"
     CDS             complement(10624..11055)
                     /locus_tag="VAH76_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEO1386187.1"
                     /translation="MQLTQFTDYSLRALIYIALKKGSCTIKDITDAYSISNNHLIKII
                     HNLSKMGLIKTIRGKNGGILMAASPETINLGQLIMELEPNFDLVPCFNKEKANCCIAP
                     ACKLKSVLYEAQRAFLQILEQYTLADVLHNPSELSVLLRIS"
     gene            11135..11509
                     /locus_tag="VAH76_001560"
     CDS             11135..11509
                     /locus_tag="VAH76_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEO1386188.1"
                     /translation="MTESLFERLGGQNAVNTAVDIFYRKMLMDDRVNYFFDDVDMEQQ
                     ILKQKGFLTMVFGGPNQYTGKSMREGHQHLLARGLNDSHVDIVIEHLGETLKELGANE
                     EDIQKVAAIANSVRGDVLGRSS"
     gene            11506..12462
                     /locus_tag="VAH76_001561"
     CDS             11506..12462
                     /locus_tag="VAH76_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEO1386189.1"
                     /translation="MNTVRFNNQSFALTPDESILQCFLRHGVEYPNSCQAGICQSCLI
                     KAKDGEINPDWQEGLPETLKSQGYFLACLAKPSTSLYVASPDNAECETQAQIVSLQRL
                     NHNVIQVKLKVEHPAPWLPGQYLSLINPEGTMRNYSIANIPADDGYIELHIKIYPQGK
                     MGQWLLQRAAINTFITIRGPFGQCYYHNPHNLAFDILLAGTGTGLAPLIGIIRCALTQ
                     KHEGTITLVHGGVTDEDIYYKEELEMLSLLFSNFRYDPCVLQSQGLYPEGSIEKRVLT
                     HLHSPNTTKVYVCGPKETTNKLKTRIFLAGVPSTNIYSDVFL"
     gene            12687..13706
                     /locus_tag="VAH76_001562"
     CDS             12687..13706
                     /locus_tag="VAH76_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO1386190.1"
                     /translation="MIVDFETHFITEACIDYLTQRQEVPKLVPELNGAYTMCFTPDVS
                     LFHTSALMEELLSLNEQRLAIMDQAGVTIQVLSLTTINGIDSCPGDENKSTALAREVN
                     DQLYSAIQKHPERFKGFASISPYDVKEGVKELERAISQLGFVGWLTHSNFGQDNYLDD
                     KIYWPLLEAAESLNIPIYLHPNVPIMREFGKYGFALGGSALGFEFDTALCLMRMILGG
                     VFDAFPKLKIMLGHLGETMPFLMERLDHLYRIPDLKPYRPSIQRIPSEVLRQNVYITT
                     SGRFFVPALRYVLEVMGEDRVLFASDYPMESLLDATRFIQDSDLSNQTKQKIFSINAK
                     NLNLI"
     gene            complement(13830..15176)
                     /locus_tag="VAH76_001563"
     CDS             complement(13830..15176)
                     /locus_tag="VAH76_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2538 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386191.1"
                     /translation="MKTKYEISQDKTEFLAKEQSSSYPGYQVSVLDLEKIVKHYQEKY
                     GIRLIINGTTPKYQALIKERQVNFEQQKQQFLELKYAKFLQIFFQPPNLNGANSPFSI
                     NKHMGAFIGFYEEIYNKVLPFLDAKGKTISGLSLEELRQLNEACQELSCKGILDAKIN
                     EFIERNFDYMGLTARESASEIKDICDELQEGEVLGYFFTGQRTSGRCHFDLYICLPGK
                     AIRPIFYNTALVRYHDLGGVFHLNFPFVEGNFFTPDLLKLYSAMDLQQLIPQADRTSC
                     GTLTMMYAKELLKDDARGLKEFTLSFTYYNEKGEKEYFFLPSPQVLRYSQISLYNEAL
                     KAILSHENDGQAGLVRKGAKKYMFHTVEKILIQSFKIAFEKEDADVLEENQKIWDMLP
                     SFQEKWQEAYKEMVAKRDVMHQGVNKYLLYSTHRMSHIASDRSINNEADADRLILR"
     gene            complement(15828..16235)
                     /locus_tag="VAH76_001564"
     CDS             complement(15828..16235)
                     /locus_tag="VAH76_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386192.1"
                     /translation="MKLIINKKNRAESMALDVEATTTVNELLIILYTHRSKRYEPLSF
                     SEYQKFISVYQCLLYGDKFINKLLPGTNLADYQLEDPCHLTWEEFAEKQCLLADAEAS
                     RILLWQANKKASKPEIEKDLMSVASSNAMNPDM"
     gene            complement(16447..16830)
                     /gene="mscL"
                     /locus_tag="VAH76_001565"
     CDS             complement(16447..16830)
                     /gene="mscL"
                     /locus_tag="VAH76_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO1386193.1"
                     /translation="MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPC
                     VGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFIIVAFAIFVLIKLINLLQRKKANE
                     PEPVTSEIQLLTEIRDLLARNSSKI"
     gene            complement(16946..>17481)
                     /locus_tag="VAH76_001566"
     CDS             complement(16946..>17481)
                     /locus_tag="VAH76_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386194.1"
                     /translation="NPFKNLKSIKKVTARLKDLGLEIGNRIFNDLDHCVYWAYHLYGK
                     SLARVQRGNEGFCNQILHKIDEDTIVIARVGAKHIPTVRKDGVVIEEGMLQRLGKDMA
                     SACFITHFPKHKNYSEQKTYQPESDMFGSYDSIECCMVQKKMIHQPDLTLWAEQDKQR
                     KDSNDYKDTKKHRCTIM"
BASE COUNT         5313 a         3515 c         3201 g         5452 t
ORIGIN      
        1 ccttcttgcc caagctaatt attttggttt aggacaaggc gcaaacgttt tgatgagtga
       61 aatttacata caagcacttc ataaacattt acaacgcgga aataaaatga aaggagtggg
      121 ttatgcaaaa gctctactgt aaccgagcgc ttttgtcatc ttgcctttga acttcctcca
      181 tttgctcaga atctaatgtc ttttttactt cactgtcctg gtttttatta agcttgtcat
      241 tatccacagc tgatttcaga acatctgatg tctcaacaga tggtgaagta tttttagggt
      301 caggctgcgt tgcctttttt aaagagaggc tatttttatg aaaattatct atttgctcaa
      361 gtgtccctct taattcagtg actaacattg agaccggact agatggtttt agatagtctt
      421 ctttcaaaaa acgaagggta atcaagtata aaagatagtt tccgaagttc ttcagcatcg
      481 aaccaagacc acgattactt ttttgttctt cagattcaat atcacgaatc agttgattag
      541 actcactttt aaaagcatca tagctttttt tgcccgcaga ccaatcctga aatacctcgt
      601 ttaatttatt cagtgcctcg cgatggcgac ctgtactttc gacatcctgg ttgagtttat
      661 ttaacccaga ctcaaaattc tttacctctt cctgaagttt ttctttattc ttaaatcgct
      721 cttgtaactc atgatgcatt gctgctcgaa ttgtcaaacc aaaaacataa tcctgagctt
      781 tacgaaattc tgcatgcgtg tcgtctagta caccgtaatt taatttaata tttgctttat
      841 caaaaatgga ttgaagaaac ggctctgtca aaacatccac aacctcactg tagctgtgat
      901 aaccttcctt gatataactg gaaatccata aattaataat ctggtttact tttttttcaa
      961 tgtctttatc cgttttatgc ttttcaatat agtccgttaa caccaccata agagaatagg
     1021 ttgtgccaga cagcgacgcc acaaaaggag tagtaggctt ggtttttgaa tatccaccca
     1081 ccactttctc attaatggct attttgggca catctctgac aggcaccaga ttatcagtaa
     1141 aattaggtga gttaggtttc ataatccctg gattcgcaga tggagtattc cccaattcct
     1201 ctctggatct gtcctttttg gggtatctct ctgattgcgc ttttaaaggt aattcaacat
     1261 tatcattcag catgacaaaa gataaaataa attgctttgc ttcatccaaa gttttgattt
     1321 cggtctcatg agttactgca tactcaggtt tcaccgcatt ataactgtct ctcaattcgt
     1381 taaatacgga aagatcactc tcttgtttaa tggctgattc aaggtgatct gcatacaagg
     1441 tttcataaag ccttctgctt tcattgtctt taggattgct ttttaatctg ggagcgacct
     1501 ctgccagcct ctggacaact tgctcactta acaaggattc atttttttta caaaaatcca
     1561 atattttttc aattgcttcc ttgtaattct ggttaaaata gagcttttct atgtcttcaa
     1621 aaaaaccaga aaactctttc tttttgccaa gtgacttttc aagatactta actgagagca
     1681 atataaaatc agactcatct aattttggag aatctaatga taatgaaata ctgagttcta
     1741 ttttttggta tgacatagga tatctgctaa attgattaaa ttttaaatca catagtacaa
     1801 aatataacaa agtgtttaat aaaagtaaat atgatgccca taaagagcaa acaacacact
     1861 ttggttaatt ttctaagcat gcataggaat taaatgattg gcaacaatac aataaggtcg
     1921 aatttaatac ttctactata cttcatatac atagaaacag aaattgagaa gtcagcatgt
     1981 ctccacgtat ttgttgctat aaccaatacc ctggcttata tcaagatttt gatgcgatgc
     2041 gggaagattt gcagcgaatt cagaagatgg gttttacgca agtttgggtc aacccttttt
     2101 atacaccttg tcaatataat cctatcccta acatggctaa tcgtattcat agtccttatg
     2161 ccatgcaaaa tgaatcgatt tatccacgct atgccaagaa tgaaaaaagc gttcagcaat
     2221 acactgcaaa agcaaatgag cttggcttaa tgcctgtatt tgatttggtc gcaagacact
     2281 tggcggtaga tcatcgtttt gtgaatggcg ataaattttt tcttgaaaaa tacaatatcg
     2341 atacgaaaaa atggtttaaa cgtcacccca atggaaattt ggtgatgcat aacatggacg
     2401 aaaattacaa tccgttgacc aataatccct ggagtgatgt ggcaacgttt aactatgatg
     2461 accctgtcat ttgcgaacaa atcatcgaat attattggaa gccgtttatc gagcgtaata
     2521 ttgagcatct tggttttcag ggaatacgta ttgatgcccc agccatggta aataacaagg
     2581 tattgtctcg actaacagaa atcgcaaagg aggcttgcca acgcaaattt caacgtgacc
     2641 cgcttattat tgcagaaacg ataggcaggg gttatatgga agaaaatctt gctttgaaag
     2701 gtattgtaac ccacacgatg aatagtgtgt tctggatgcc gggccccgaa ggtggctatg
     2761 gttattcata taacttatgg cagcaggatg acaattggtt tactcaaaat aaaggattat
     2821 tacagcaggt tggtcctaca attggttttc ctggctctca tgatgagcca cgctatattc
     2881 agcaactggt ggaaaaaggt atccatgatg acaaattact tgcaaaacgc atgcgcgaaa
     2941 aactggctgt cagcgcattt tgttctgatg gcggttggat tttacaatac ggtgatgaat
     3001 atggtgctac caagccagta aacgtttttg atccaacccc tgttgaatat catcaacaaa
     3061 atcatctgag acgatttgat ctgtcagact atatctctga aattaataca acacttgccc
     3121 agctacccaa tcctcacttc cccgaatggg cgcagcgggt ctttcttccg aatcatccag
     3181 atttggtaac ctttattgtt caccagggtt ggggttatac tgggacctca ttcgttattg
     3241 cgacaaacac cgatccctct cagcaaatcc aattaacaga aaaagaactt ggagaaataa
     3301 tgctggcaaa tggccgaaat aatacgactg aaaaacagat gaaaccccaa gtgctttatt
     3361 tatgtggcgc agttcgcgca tcacctgaat tgaaaaaaga gatccaaatt gttgcatcaa
     3421 ggccaactgt aagtgaaaaa tctcctcatt ttttccagag ccatcctgaa gaaaaaaaaa
     3481 cgggcgaaaa gttacattcg gacaatacac caccccaatt taaataacag gtaccaggct
     3541 tagctctaaa accacgttta ctggtgagtt gattgttatc atattgctta ctcaccaatc
     3601 agcacatcac tttgataagg aagcagcatg gctttggtga ttttatcgtt cctgttttta
     3661 aaaaaagcat gggcctctct cttgcataaa ctgattattt tgttttaggg caaggtgcaa
     3721 acgttttgag gagcagtgtt tacatccagt aaataagcac ctcagaaacg tttgaaaagc
     3781 gacaggttat gccaggtttt ctacattgct acaagtttac atgtaacttc gctaattgtt
     3841 tctttttgct taatgatttt atccatttct tgcacaaaga ggatgacatc atcaagaatg
     3901 aaattgcgtt tatgagattt cgaatactct atatacctct gagcaatgtt tttacacttc
     3961 tctggcgtat atctttcgtc gccctcaagt cctttctgat acatctgggc gagtgctctc
     4021 gctacgcgtg gaccagactg cttgtttttt cgaatataag ggtttagttc tgtagggtat
     4081 ggttttgggc ttacgtcttc tgatacctta agcagggtat gtttcgaaaa ttgacttaat
     4141 accccctttt gagattggtc agaattgact ttctcctcag atgatttaat caaggacaag
     4201 tcctcttttt tctgggatat tacaggactg cgttccggac tgtacaatcc aaatttagct
     4261 aggcctgtgc tgagtttatt ttcaaccata agggctggaa tgcattcgag aaaattggaa
     4321 aaaagagtac caagcgacat cctcaaggct ggttctgatg ctaatttagg tgttgcttta
     4381 gccagaagct gaaaaaataa ttcaatttct ttttgttttg taacagcagt tggactggtt
     4441 ttaataagct ctatctgctt catcaaatcg ctaagccaat cacttatcga agagtatttt
     4501 tctaccccaa gctctttatt gccatactct gactgcatcc gctccagcat ttcgagtagt
     4561 ttttcttctt tgtccgttag ttttgaggta aatatcgcat caggaatgac agaagtccaa
     4621 tgaacattgc cttcattatt taatatcatt tccggactgc ctgcctgata ggacggactt
     4681 gctccatttc tatgaacatg caaaataatt tcatgatcat agacaagctc aattcttccc
     4741 tcatggttac gtaccatgcg ttcaccttgt atggcgcgat gtaaaaccat cagtgtttct
     4801 tcactacccc atacgaactc tcttcgaaga tgctctctgt attcatcaag atgtttttca
     4861 ttatcagcta ataaaaaatc tatcgtttct tttcttagga tgttatctaa aatagtcgcc
     4921 tgatgaacct gactctcttt ttcagttaag ctttgctctt tgagttcttg ttttttattt
     4981 tcccattggc gattgaattc ttctatgacg gaaggtgttc ttgttaaaat atattcctga
     5041 agagcatcca acaaaccact taccttgtat atctcggcct cagtataatc aggattcgag
     5101 aaatcattgt ctattaacag cgtcaactcg gattcattga tttgtagtga gcgtttaaaa
     5161 ccaaattcca ccgcataatg gagagatgaa aataaaggcg tatcatgagg tgaagatttg
     5221 aattcgactt tggtatgttc cgctgccaaa tctctcgttg ccctgccaag aatgggttct
     5281 ataattgttt tgatttcatc gcgggtaaac gccctatcag ggtctttgga taatagtttt
     5341 cgtaaatggg ccctatcttc ttcctttaat cctaatttgt taaaaatatc ttcagtaatc
     5401 ctgacattat tttttgcccg caaataatac attaatgaaa tactgtaagc ataatacaag
     5461 caattgcctt taccactatt atccacatta aacatattga ctcctttttg taaattcaaa
     5521 accctcacca cattaatagt cttttattgc ttttcctcct tggtttgatt tcattatttg
     5581 aaagattcat gagttctttt ctggtaatgg actgatactc agtgatataa gtgagtaaat
     5641 cgataaatag tttatttgat acgaaaagaa ttcctttatg ggtggattcg gagtcattca
     5701 tttgaacccg gtgaccagtg aaattggttt gcagcggggt attatgattt atgattaaat
     5761 cccaacatat tgacacaaca ttaatccctt agtttttatt tgatataaaa tgcaatgtat
     5821 acgatcaaaa tgattcacgc aatactttga tctcattttc cgatcagtga gtgcgattaa
     5881 tgccaaagtg atttttatat gtaaatcatt gggttaattc acagggaaaa attggctata
     5941 aaattaactg tcgctaacag gtgttgacag gtttaccata gtcctgtata cataatatac
     6001 aaattaaatt ttttgtgatt attcatgttc tacactacga caccaaatca gttaaataaa
     6061 aaagcaagcc ttgtacaggc ttttgcttgc tttttttatt ttagcttttt cagccctgag
     6121 ctgggtggta ggtaggcaat actaacactc caacccccag ctgagtctgg gggtttttga
     6181 tacccaagat ccccagtcta ataagggggt tgggtacaaa agaggagtgt gaacgatgaa
     6241 ccaccaatgt caaattcatc atccatctaa agcgctttct ggcttgagga ggatacgatg
     6301 agttattcca tcttaaaaaa actgccacca atagaaaaaa tcatccggga attgccacta
     6361 tcccactccg cctatctaca aattgctcaa gaccggcaag agatcaaagc tattttggaa
     6421 gggcgagata aacgtctttt gatgatagtt ggtccttgtt cagcatggcc taaaaaagcg
     6481 gtattggaat acgctgagcg tttggtacaa ctcaacagca aagtaaaaca cgctttaaaa
     6541 ttaatcatgc gcgtttacat tcaaaagcca agaaccacca aaggctggac tggaccggtg
     6601 aatcaacccg atttattttc aactcctgat attgagacag gtataaaata tacccgggac
     6661 atgatgataa aagtcattca aatggggctc cctatcgctg atgaggctct tttcacccat
     6721 aatgccaaag gatttcttga acttctgtcc tgggttgcaa taggagcccg tagttcagag
     6781 gatcaagagc atcgaatttt tgcctccgcc cttgactgtg ctgtaggatt aaaaaaccct
     6841 acacatggtt cactcgctgt cggagttaac agcctggttg catcacagca tcctcatgtc
     6901 gctgtcttcg atggctatga agtgcaaacc catggtaatc atcatgctca tctcgttctg
     6961 cgtggttcaa accaagctcc caattactcc gtttctcatc tggaggaagt catgtactac
     7021 atgggcatgc atcaaatagt gaacccttct gtcattattg atgttagcca tgacaactgt
     7081 ctggtgaatg gaaaaaaaaa tcatcagcta caaccagaga ttgcatttaa tattctggaa
     7141 agcatcaaaa acagacctga tttaaaacaa ctggtcaagg ggttcatggt agaaagtttc
     7201 atcaaggaag gtaatcaaaa attagattct atcaatcccg ataacattga tttaagcggg
     7261 ttatccgtta ccgatccatg cctcagttgg gagaagacag aatcgttcct gttggagctg
     7321 gcggagaggg aagcattatg aataatctcg ttgaaaaaaa agaagccttt cgctttggtg
     7381 tttcagggga tattggatca ttttctgagg aagcggcttt gctttatgcc aggaaaaaag
     7441 gctttaatgc ccatctcgtt catttgctgg atatggaagg cgttttaaat gccattgaga
     7501 atgaaacgat agagctgggc atattacccg ttgttaacct tttaggagga ttggtaaagc
     7561 cagcatttca ggctatgggc aaatattcat ttactcccat cgatgagatt tggcatgaca
     7621 taaaccaatg cctgttagtt aagacaaata ctcaaaatca taaaatcaca catattgtgt
     7681 cccatcctca aggctttgcc caatgcaaaa aattcctaaa aaacaaattt aagaatgcga
     7741 aaaaaataga atggatcgat acagcaaaag cagcaaagga tttggcggaa ggaatattgc
     7801 caccccatgc cgcagttatt gcgtcggcac gttgtgcacc actttatgat ttgaaaatca
     7861 gtgcgagaaa tattcaggac agccatctga acagaacggc ttttatatta gttaaaaaat
     7921 aaagtgacaa ggtccctatc cgctaaggag ttcatcatga gtacgattga agaactaaga
     7981 aaacaaattg agcaaactga tgcagacatc attgaaaagt tggctcaacg ccaagaattg
     8041 tctaaacaaa tcggagcttt aaaggctaaa gaaggaaaaa aaataataga tcgcctgcgt
     8101 gaaaaaaaat tgtttgaata ttatgacttt ttatccaggc aatatcactt gcagcaggat
     8161 ttcgtgaatc aattattcaa gataattatc tcaaactcaa aaaaggccca aaaataatga
     8221 accattatga aaaaatgagc ttgttaaaag attccgcaac agttgccatc aattctctag
     8281 cccaacaaaa aataaacgcg ggaatcaaaa ttcataacct cagtgctgga gaacccaaac
     8341 tgccacccca tccctccata ataaatgccg tcacacaagc tatggaacaa ggacataccc
     8401 tttatcctcc aataatggga attcctgaac tacgtcatat gggctgtgaa tggatgtata
     8461 gatcctatga ttgctcattc caaaatgagc aatgcctggt tgtgaatggc ggaaaactcg
     8521 gcatttattt actcatgcaa ttattactgc agcctaacga cgaagtcatt attccttctc
     8581 cctactgggt ttcctatcca gccatggtta gtctctttgg cggcaccccc gtgtcagttg
     8641 aaacgacaga agccgaagaa tggaaattaa cgccaaaggc attgcaaaaa gcgtgtacca
     8701 caaaaagtaa aatactcatt ctgaataatg ccaccaatcc caccggagct ctgtataccc
     8761 agtccgagct gattcactta ttgcaaattg ccaagaagca taatctttta gtgatctccg
     8821 atgaagttta cagcgaactg acctatgatg ggcatcatta cgtatcctgt ggttctttac
     8881 cccaatttcg ggagcgtgtc atcatcattc aaagctgttc aaaaaacttt tccatgaccg
     8941 ggtggcgagt gggttttatt tttgccccgg aaatattcat caaaccttta agcgccctgg
     9001 tcagtcaaag cactagtgga gtgaccaccc ttagccaatg ggccgctgta gctgcgctgc
     9061 aagaagtaaa tcaagtcaat atctgggttc aacaaaacat ggaaaaaaga agaaattgcc
     9121 tcctccgcgc cttgcatgac tatctgggat tgactataac acctccagtt tcctcacttt
     9181 atgtttttct ttccctaaag gatattggct gcaaaaatca aaattccgag gagttttgta
     9241 aacaagcttt ggataaggca aatgttgcga tggtgccggg aatcgccttt ggtaaagagg
     9301 gttacgtccg cttgtctttt ggaggcaatg aagacgattt aaaatcaggt atttatgccc
     9361 tggcgcaatg gctgcatcgc tgattaaagt aattcattac tttgcaagaa gcattgctag
     9421 acctcttgcc taatctgatt attttcagga cttacgaaaa actccaaggt caaggcaaaa
     9481 aatgttttta atgagggagt ttagataaac taaatgaccg aattaaaaac attttttaac
     9541 aaagagatgg gggtttttcg taagtcctga ttttatttta ctgcagtttt gcagttaata
     9601 acctgctcca tataacggct ctataaaatt gatgcattcg acaataggct aaaaaaccat
     9661 tattttattt atcaatacat caggataaaa aaccatgcca agagaatacg ataatttaat
     9721 ctgctacaaa caactatcca ttaatgacca tggaaaactt aaattttttc ttgaaaagca
     9781 cagcactaaa gaaggaacat ggggcaaact ttccctaaac gaaggtgaaa ttgattttat
     9841 tttccagaat ggcttgggtc aggaactgtc gcgtattcgt cttgataaaa ataataggca
     9901 actatctatt cctccggcct cctggcataa aatcattccg atcagcgcat cgttcgatgc
     9961 tcagcttggt ttttattgca aacctcatcg ctactttaac aaaaaatacg gcttaactga
    10021 ggtgcaccgt gacttattgt atgtgtatca tacctacttg tcgcatttag gctcactaac
    10081 cactctggat gtcggttgtg gttccggtag aaatctgttg tatttggccc aacttggcca
    10141 tacagtgact ggaattgata tcaatcaaac ggcactggaa aatattcaaa taatcgctca
    10201 aaaagaaaac ctgagtccgc ttaatatttt gtcgcaggat ttaaatcaac ctcaatccct
    10261 tgcaccagat cattacgatt ttgtctgctc taccgtgact ctccaatttt taaatgcctc
    10321 acgtatcccc tctttattga ttgagttgca ggaggcaacg aagaaaaatg gctatcattt
    10381 tctggttttt cccgtccaat ctgaacttta ttcactccca agctcattca cgtttttacc
    10441 aaaacaagaa gagctgtatc atttttacca ggacagcggc tggtctatcc tcgaatacaa
    10501 agaatcagta ggacatcttc ataaacagga tgaattggga agaccagtgc aaggattatt
    10561 tgctctgttg cttgcccaga aagttatata aagatcactt tcataaaata ttactctgga
    10621 ttgctaactg atcctcaata aaacagacaa ttcacttgga ttatgtaaaa catcagccag
    10681 agtatactgc tctaaaatct gcaggaaagc acgttgcgct tcatacaaaa cgctcttgag
    10741 tttacatgct ggggcaatac agcagtttgc cttttctttg ttaaaacagg gaaccaaatc
    10801 gaaattgggc tcaagctcca taatcaattg gcctaaatta atggtttcag gtgaggccgc
    10861 catcaaaata ccgccgtttt tcccccgtat tgtcttaatt aaacccatct tcgataaatt
    10921 atgaatgatt ttgatgagat ggttgtttga tatggaatag gcatctgtaa tgtccttaat
    10981 ggtacatgag cctttcttta aggcaatgta gatcaaagca cgcagggaat aatcagtaaa
    11041 ttgagtcaat tgcataaaaa atccttctgg tttgttgacg tctattattt ctttttataa
    11101 gatgtatttc aaatgcatct ttaaggagta tatcatgact gaatcattgt ttgagcgatt
    11161 aggtggccaa aatgccgtca acacggctgt ggatattttc taccgcaaaa tgctcatgga
    11221 tgacagggtg aattattttt ttgatgacgt cgatatggag caacaaatcc ttaagcaaaa
    11281 aggattttta accatggtgt ttggcgggcc aaatcaatac actggaaaaa gtatgcggga
    11341 aggacatcag catctacttg ccagaggctt aaatgactca catgtggata ttgtaatcga
    11401 gcatttaggg gaaaccctta aggaattggg cgccaatgaa gaagacattc agaaagtagc
    11461 tgcaatcgcc aatagtgttc gtggcgatgt attggggcgc tcatcatgaa taccgtacga
    11521 tttaacaatc aatcttttgc attaactccg gatgaaagca tcttgcaatg ttttttgcgc
    11581 cacggggtag aataccctaa ctcctgccag gcaggcattt gccagtcatg cctgattaaa
    11641 gccaaagacg gagagattaa tccagactgg caagaaggat tacccgaaac actcaaatct
    11701 caaggatatt tcctggcttg tcttgccaaa ccaagtactt cgctttatgt cgcatctcct
    11761 gacaatgccg aatgcgagac acaggctcaa atagtgagcc tgcaacgctt aaatcacaat
    11821 gttattcaag tcaaactgaa ggtggaacat cctgccccat ggctacccgg acaatacctg
    11881 tcgctcatta atccggaagg aacaatgcgc aattattcca tagccaatat ccctgctgat
    11941 gatggataca ttgaactgca catcaaaatt tacccgcaag gcaagatggg acaatggctt
    12001 ttgcaacgcg cagccattaa tacattcatc accatccgag gcccttttgg tcaatgttat
    12061 tatcataatc cacataatct ggcttttgat atattgctgg cagggacagg gactggcctc
    12121 gctccgctga taggtatcat cagatgcgca ttaacacaaa agcatgaagg aacaattacc
    12181 ctggtgcatg gcggagttac tgatgaggat atctattaca aggaagaact ggaaatgctg
    12241 tctttattgt tcagcaactt ccgctatgat ccctgtgtgc tgcaaagtca agggctttat
    12301 ccagaaggct caattgaaaa acgggtgcta actcacctgc actctcccaa cactacaaaa
    12361 gtgtatgtct gtggtccaaa ggaaacaacc aataaattga aaacacgaat ttttcttgcg
    12421 ggcgttcctt caacaaatat ttatagtgat gtttttttat aatatttaaa tagccctggt
    12481 gatttataat ctggtgcaaa ccagattatg tttctcgctc atcaaaatga tctcttcttt
    12541 ccgctgatag catttctttt ctttaagaca ttttccggca atttgccatc agaactttta
    12601 tagccatgtg gcgatatcta atcttccggc tatacttaag catataattt tcaatcgttt
    12661 ttctcacgtc taaaaggatt acgttcatga tcgtcgattt tgaaacgcac ttcatcactg
    12721 aagcttgtat tgattatctg actcaacgcc aggaagttcc caaattggtt ccagagctca
    12781 atggagctta tacaatgtgt tttacgcctg atgtttcctt atttcacaca agtgcattaa
    12841 tggaagaatt attaagttta aacgagcaac gactggcaat tatggaccaa gcaggggtga
    12901 caatccaggt attaagtttg actactatca atgggattga ctcctgccct ggagatgaaa
    12961 acaaaagcac cgctttagca agagaagtca atgaccagct ttactctgcc attcaaaaac
    13021 atcctgaacg atttaaaggt tttgcttcta tatcgcctta tgacgtcaaa gagggtgtaa
    13081 aagaattgga aagagcgata tctcaattag gctttgtagg ttggcttaca cactccaatt
    13141 ttggccagga taattacctg gatgataaaa tctattggcc attgctggaa gccgcggaaa
    13201 gtttaaacat tcctatctac ctgcatccga atgtacccat catgcgcgaa ttcggtaaat
    13261 atggttttgc cttgggagga agtgctttag ggtttgaatt tgacacggct ttatgtctga
    13321 tgcgaatgat tcttggaggt gtattcgatg cgtttcccaa attaaaaatt atgctggggc
    13381 acttgggcga aaccatgcct tttttaatgg agcgtctcga tcatttatac cgtataccag
    13441 acttgaaacc ttatcgacct tctattcagc gtattccctc agaggtatta cgtcaaaatg
    13501 tttacataac caccagtggg cgtttcttcg ttcccgcctt acgttatgtt cttgaggtca
    13561 tgggagagga cagggtattg tttgcctccg attacccaat ggaatcctta cttgacgcca
    13621 ctcgatttat tcaagattcc gatttatcaa accaaacgaa gcaaaaaatt ttctctataa
    13681 atgccaagaa tttgaactta atatgaatca tctctcttgc atcacatcag taaggagctg
    13741 attttcttgc agacattaat tgagccaggc tcaattaatg tcatgctttc tataaataaa
    13801 atacgaacag tttcattctg caatattttt taacgaagaa ttaatctatc agcatccgct
    13861 tcattgttta tggatcgatc gcttgcaata tggctcatcc gatgggtgga ataaagcaaa
    13921 tacttattta ccccttgatg catcacatcg cgtttggcaa ccatttcctt ataggcttcc
    13981 tgccattttt cctgaaaact gggtaacata tcccaaattt tttgattttc ctccagaaca
    14041 tcggcatctt ctttctcaaa tgcaatctta aaagattgga tgagaatttt ttcgacagtg
    14101 tgaaacatat attttttagc gcctttcctg accaaaccag cctgcccatc attttcatga
    14161 gataaaatgg ctttcagcgc ttcattatat aaacttattt gagaatatcg taatacttgt
    14221 ggtgatggga gaaaaaaata ttccttttct cctttctcat tataataagt aaaactcaac
    14281 gtaaactctt tcagccctct cgcatcatct tttaataact ccttggcata catcattgtc
    14341 aaagtaccac atgaagttct gtcagcttga gggattagct gttgcaagtc cattgcagaa
    14401 tataatttta acaaatcagg tgtaaaaaaa ttgccttcga caaaaggaaa attcaaatga
    14461 aacacccctc caagatcatg atatctgaca agcgctgtgt tataaaatat cggtcttatg
    14521 gccttaccag gtaaacagat atagagatcg aaatgacatc tcccactggt acgttgacca
    14581 gtaaagaaat aacccaaaac ctctccctct tgcagttcat cacagatgtc ttttatttca
    14641 cttgcgcttt ccctggcagt taaccccata taatcaaaat ttcgctctat aaattcatta
    14701 atttttgcat caagtattcc tttgcatgac agctcctggc aggcttcatt gagttgtctt
    14761 aactcttcca gggataatcc agaaatggtt tttccttttg catccagaaa tggaagaact
    14821 ttattgtata tttcttcgta aaaacctata aatgcgccca tatgtttatt gatagaaaaa
    14881 ggagagtttg cgccattcaa attaggtggt tgaaaaaata tttgtaaaaa tttggcgtat
    14941 ttgagttcca ggaattgctg tttttgttgt tcaaaattaa cctggcgctc tttaatcaga
    15001 gcctgatatt tgggtgtcgt gccattaata atcaagcgaa taccgtattt ttcctgataa
    15061 tgcttaacta ttttttcgag atccaaaacg ctgacttgat accctggata agagctactt
    15121 tgttctttag ccaaaaactc agttttatct tgacttattt catattttgt tttcataaaa
    15181 aaccctggtt gcagcttgta tcattactaa ttcatgaatt tttctcatca cctggactgg
    15241 aatcaattaa ttgtccaccg ggatgatttt cattgatttc ataatgacaa ttccttgctc
    15301 tatctcaatg aaacaactga ttattaaaat cattattttt cattatgaat tccttataaa
    15361 ttaacaaacc ctctgcctaa taaagactga cagaggccat ggatatttca atttagagac
    15421 aaaaagaaag gaaacgacga tttgttaagt aacgcctcgc tgaattaaag ttaataaact
    15481 actctcaatc accaaatccg agagtaaatc gctcattttc tttgcctcag cgacgaaccc
    15541 gtttacttaa ttaaagctaa gtgttaccac tagtcctccg tatatccttg tggagaagat
    15601 aggccgcagc aaaaagatca ttttttatga tcttttagtg agcaaaattt taacaataaa
    15661 agcttaagaa tttattaaat gggcaacacc ttatattgag gccatagggg actcaagacc
    15721 tttcactggt taactgattg gatgtactta atttttttca ttgcatgact caagtaatga
    15781 attacaaaac gaacactcac tatgtaatac aggacatttt attcttgtta catatcagga
    15841 ttcatagcat tactgcttgc aacggacata agatcttttt caatttcagg tttacttgct
    15901 ttcttattag cttgccaaag aaggattcta ctggcctctg catccgctaa aagacactgc
    15961 ttctcagcaa actcctccca ggtcaaatga catggatctt caagctgata atcagctaaa
    16021 ttggtcccag gaagtaattt atttataaat ttatctccat acaataaaca ttggtaaacg
    16081 gaaataaatt tttgatactc actaaaagac aaaggctcat accttttact gcgatgagta
    16141 taaagaatta taagcaactc attcactgtc gttgttgctt caacatccaa agccatcgac
    16201 tccgccctat tttttttatt aattataagt ttcatttgcg atcattaagt aaaaattagc
    16261 cttgtctgct agtatagttt aaataacaat aaatcaatat atggctttgt gagatttaag
    16321 tggccgattg aaacaccaga attctgtata catctcaccg aaataaaaca taagctgttt
    16381 aacaagccat tttgaatggg ggtatctgac tgcgaataaa tagaggttta tttttgccag
    16441 ccaatttcag attttactgc tatttcttgc aagcagatca cgtatttcag taagtaattg
    16501 gatttccgaa gtgactggtt ctggttcatt tgcttttttt ctttgcagca gattgattaa
    16561 tttgatgagt acaaaaatag caaaagcaac gataataaaa tcaatgacgt tttgtaaaaa
    16621 atttccccac ttgatttgag cgtctcctac agtaaaactc aatcctgcaa tattgacgcc
    16681 tcccaataac aaaccaacgc agggcatgat aattccatcg accaaagcag tgacaatttt
    16741 attaaaagca acacccatta caacagcaac agccaaatcc atcacgttgc ctcgcatggc
    16801 aaattcttta aattccttta gtaaactcat cagacatccc tatcatgatt aagttatgcg
    16861 atgaaatgta tattcgcaaa atttttccaa tctttcaatt acagattgtt ctgacgcctg
    16921 ttagcttgta gaaaggaact taaatttaca ttattgtgca acggtgtttt ttagtatctt
    16981 tgtagtcatt ggaatctttt ctttgtttat cttgttcagc ccatagagtc aaatccggtt
    17041 gatgaatcat cttcttttga accatgcaac attctataga atcataactt ccaaacatat
    17101 cggactctgg ctgatacgtt ttttgttctg aataattctt gtgtttaggg aagtgtgtaa
    17161 taaaacaggc ggaagccatg tctttgccta accgttgcag catcccctct tcaataacta
    17221 ctccatcttt tcgtaccgta gggatatgtt tggcccctac tctggcaata acgatagtat
    17281 cttcatctat cttatgcaaa atttgattac aaaagccttc attaccccgt tgtactctgg
    17341 ctaatgattt tccatagaga tggtaggccc agtaaacaca atgatcaagg tcattaaata
    17401 ttcgattccc tatttccaaa cccaaatctt ttaaacgtgc cgtcactttt ttaatagatt
    17461 taaggttttt aaatggatta c
//
LOCUS       DATNSN010000058        17284 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.058, whole genome shotgun sequence.
ACCESSION   DATNSN010000058 DATNSN010000000
VERSION     DATNSN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.058"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            114..1004
                     /locus_tag="VAH76_001567"
     CDS             114..1004
                     /locus_tag="VAH76_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HEO1386195.1"
                     /translation="MPFAHLLLALLVVFVWGINFIFVKLSLEEFSPLLLCAVRFLLAS
                     VPAVFFIKPPAVPFKIIAGYGLIMFALQFALLFIGLRVGMTPGVASLLMQVQVFFSMF
                     FAIIFLGEQPQVGQIIGALIAFIGIGVVALHFDHNVSLMGFLCILAAAASWGIGNLIT
                     KKIHSVNLIAVIVWSSFVACLPMFILSLVFEGPESFVTAYEHMTWKGIFSVLYIVYIS
                     TWVGYGVWNWLISRYPVGMVVPFTLLVPVVGILSSVFILGEPFYLWKLVAGLLVISGL
                     CINLLTTRLFVAKTQQPETA"
     gene            1171..1983
                     /locus_tag="VAH76_001568"
     CDS             1171..1983
                     /locus_tag="VAH76_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEO1386196.1"
                     /translation="MSDLKNIFLCADDFGLNHGITQGILKMARLQRLSAVSCIVNASS
                     FDVDAPDLLALKNGVRTGLHFNLTEGYFLSHPDKLCFGLRELLIRSHLGILNSRLISD
                     ELNTQLDHFIKVMGKLPDFIDGHQHIHQFPGIRRIIIDMYEQRLKGKSIFIRSTYPSV
                     NMSGYQAKTKILGITGGKILSSQLKKTNIPHNPYFAGIYDFSDRTNYRSLFRQWLNNV
                     PTNTLIMCHPGEGENSDIIAEARARELNYFLSDEFMQDCEEYQVNLTTGPKS"
     gene            complement(1960..2349)
                     /locus_tag="VAH76_001569"
     CDS             complement(1960..2349)
                     /locus_tag="VAH76_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO1386197.1"
                     /translation="MTTALKLSHRLIYFTGVGLCAAVVHLLIVFNLVNFLNLQPLLSN
                     AIAFLIAFNVSYLGHKYFTFNQLQDEKQLSLPHFFLVAASAGLLNETLYYLLLRFTTL
                     HYIAALVFVLSIVAVYNYIFSRFWACR"
     gene            complement(2333..3292)
                     /locus_tag="VAH76_001570"
     CDS             complement(2333..3292)
                     /locus_tag="VAH76_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO1386198.1"
                     /translation="MSKTAKHKKLISCVVPVFNEEVLIAEFIAALDKTLKSISYPYEI
                     LLIDDGSQDNTFAIIQTLRKEYSLRCIRFSRNFGKEKALSAGLDHARGDAVILLDSDF
                     QHPLELLSEFIAKWEDGYDMVYAVRQNRSDESWLKRVCAKIFYHLTSKINRINIPANA
                     GDFRLLDRKIVNALQTLPERNRFMKGLYSWVGFKQIAVPFNVHPRKSGSSQWSFYSLL
                     DLAITGITSFTAFPLRMISLGGMGVAALALIYAAWIVLSTLIFGIETPGWATLVTAIS
                     FFGGLQLFAIGVVGEYIGRVFDEVKHRPHYIIDEESSFDDHCS"
     gene            3439..4116
                     /locus_tag="VAH76_001571"
     CDS             3439..4116
                     /locus_tag="VAH76_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEO1386199.1"
                     /translation="MNDSGWFLISTALLLGMRHGFDLDHLATIDAISRTVRYKPSLSR
                     MVGFLFSLGHGLVVIIVSLIIGGGIISASTPEWLNEVGNIVSVTCLLLFGFLTLWNTV
                     HYSSSAIIPTNLRSYLFRKLADNNVHPLMILLIGALFALSFDTISQIVLFSLAASALS
                     GFLFSGLLGFVFMLGMMMSDGLNGFFVASLIRRADGISLLFSRLVGFGIAFFSIGIAI
                     INCCKLL"
     gene            4128..4469
                     /gene="hypA"
                     /locus_tag="VAH76_001572"
     CDS             4128..4469
                     /gene="hypA"
                     /locus_tag="VAH76_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEO1386200.1"
                     /translation="MHELWLCKRIVEIIKQQATGNKCRMVKKIVLEIGQLVAVDKHAL
                     NFSFKVITQGTIAQNAELSIVEIPGEAICNSCQQIVPMKQYYDECLVCGNHSLTLTKG
                     EELKVKSMVVE"
     gene            4469..5227
                     /gene="hypB"
                     /locus_tag="VAH76_001573"
     CDS             4469..5227
                     /gene="hypB"
                     /locus_tag="VAH76_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEO1386201.1"
                     /translation="MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQN
                     NEFAAHNRDYFLRHKILVLNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCD
                     AEMLKSSGGNAWQINTGKVCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHL
                     GEQFRIVTLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNP
                     SIDIITLSAVNGVGLKDWYDWLLHSFRLNVQASS"
     gene            5243..7468
                     /gene="hypF"
                     /locus_tag="VAH76_001574"
     CDS             5243..7468
                     /gene="hypF"
                     /locus_tag="VAH76_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEO1386202.1"
                     /translation="MELERVQISIQGLVQGVGFRPCVYKLAKQMELTGFVQNNASGVL
                     IEIQGNYLSQFIPKLREQLPPLAAIHNLEFTSLPIVPNERAFEILDSQQGKINTVITP
                     DVCICSECLGELFDPQSRYYRYPFLNCTHCGPRFTITRNLPYDRCQTSMSQFPLCQSC
                     QSDYLNPDNRRYHAQPTACSQCGPQLALSVDKVAQRIRDGEIIALKGLGGYQLICDAR
                     NEGAISQLRLRKSREAKPFALMVVNAKSAEKIAEVSPQARLVLESRERPIVLLRKKKA
                     LLPDGIAPGLNHFGIMLPSTPLHYLLFNSFLGNPDYCQWLDEYHSMVLVVTSANIGGE
                     PLIIDDKKAEQGLISIADKIISHDREIITRTDDSVVRMVNDTSVLIRRARGYVPAPLR
                     LPYEIPPTVALGGHLKNTFCITRGEEAFVSQHIGSLNNKATIEYFHESLNHLLKFLAV
                     KPERIAHDWHPDFYTSRLAQKYDIPVYGVQHHHAHLASVVAEHGIQEPVLGLVLDGYG
                     FGSDGEAWGGELLLLEKAEFKRVGHFFPIPQPGGELAAREPWRMAAGILHILGRGDEI
                     SQRFKEQPQSPFLLQLLEKKIHCPLTTSCGRLFDAVSALLGVEFLSRFEGHAAMRLES
                     MVTHPQVLNKGWTINDNVFDMRPTLQFIANTTEQQLGANLFHGTLIAGLSEWVTGICR
                     EKGIHMLLLSGGCFLNQVLAEGLINSLIKSGIKPLLPKLLPPNDGGIALGQAWIVGIK
                     E"
     gene            7478..7705
                     /locus_tag="VAH76_001575"
     CDS             7478..7705
                     /locus_tag="VAH76_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEO1386203.1"
                     /translation="MCLALPAQIDQVLEDNKAIVNLGGITKEISTVLLEQVSEGEYVI
                     IHAGFALTKLDEREANKTLSLFAHMLKDGLK"
     gene            7702..8808
                     /gene="hypD"
                     /locus_tag="VAH76_001576"
     CDS             7702..8808
                     /gene="hypD"
                     /locus_tag="VAH76_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEO1386204.1"
                     /translation="MKYIEEFRNGAYAQAIINEIIATADPQRHYQFMEFCGGHTHAIH
                     RYGIPSLLPNNVEMIHGPGCPVCVLPIARIDKAIALASMQQVILCSYADMLRVPGSGQ
                     KSLLTAKACGADVRMVYSANDTLKIAQENPNKDIVFFAIGFETTIPPTAFVIKQAKKL
                     NITNFSVFCNHVLTPIAMDSLLQANEVALDGFVGPAHVSIVIGSFPYEQVCKKYQKPI
                     VISGFEPLDVLHSILMLIQQLNEGRCDVEIQYTRAVNRKGNLLSQHIMAEVLEIRDRF
                     EWRGLGFIPSSALKIKESFSEFDAEQRFELPESAAQEHKQCLCGEVLRGVKKPTDCKL
                     FAKACLPENPLGSCMVSSEGACAAVYAYGRVNAL"
     gene            8805..9863
                     /gene="hypE"
                     /locus_tag="VAH76_001577"
     CDS             8805..9863
                     /gene="hypE"
                     /locus_tag="VAH76_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEO1386205.1"
                     /translation="MNEILITKKLTGPKLNFKEGTINLTHGSGGRAMAQLIEQLFLTA
                     FDNEWLAQKNDQACFIVPSGRMVMATDAHVISPLFFPGGDIGSLSVHGTVNDVAMSGA
                     KPLYLTASFILEEGFPLNDLRKIVYSMADAARQAGVAIIAGDTKVVERGKGDGIFITT
                     TGVGIVPAGIHISGDAAKPGDKIIVSGFIGDHGVAIMAHRNNLEFQTKVQSDTASLHG
                     LVGEMVKAVPELHCLRDPTRGGLATTLNELALQSKVGFVIDESKIPIRTEVASACELL
                     GLDPLYVANEGKLIAICPPEKAERLLTAMKAHLLGLHAEIIGEVIEDHRCFVQLKTKL
                     GGLRIVDWLAGEQLPRIC"
     gene            9899..11038
                     /locus_tag="VAH76_001578"
     CDS             9899..11038
                     /locus_tag="VAH76_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEO1386206.1"
                     /translation="MNDKRNCFLPHDKLQHLINSLQNAGYSCVGPQVRDGAIVYDVLT
                     RAEQLPWGMRDHQEPGGYRLEQISERKAFAFANGPQGIKPLLFKPQETVWRVERDEQG
                     KLCFKTHQPDETPVAIIGSRACDLAAMSIQDKVFIESNNPDPRYKRRREQLFVVAVNC
                     TYSSANCFCVSAGTGPAVNNSFDILMTEIEDGFVVEAGSDKGKKIINQLDLHDAKSAQ
                     CTDAQNNVKQAAALQTKRIPLDNKRDLRDLLFSNLNHTRWDEVAERCLSCGNCTLVCP
                     TCFCHSEVEKPSLDGCTSEHHREWDSCFTTGHTYLNGKIIRDDTKKQYRQWLTHKVGS
                     WFDQFDTSGCVGCGRCVTWCPVGIDITEELAAISGESNARKIENE"
     gene            11031..11876
                     /locus_tag="VAH76_001579"
     CDS             11031..11876
                     /locus_tag="VAH76_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO1386207.1"
                     /translation="MNKVVYDPYLPKEVEIIQRTQESSSIFTLRLRFTDEDHHKQFQF
                     YPGQFNMLYLYGVGEVAISIVSDPRKKTFLSHTIRAVGRVTKAMQKLQVGDRLGIRGP
                     FGVGWPLQKTIGKDIIVLTGGLGCAPSVSIINYILGRRRHYGKLSILQGVKHSDDFIF
                     RKQYAKWQKSDHTEVYIAADQAGPKWPWGVGYVTDLIDHIMIQPDNSVVMMCGPEMMM
                     NTAVKVFTQKGILENDIYLSMERNMECGIGHCGHCQYGGLFVCKDGPVFAYSQIKELF
                     KESGF"
     gene            11894..12679
                     /locus_tag="VAH76_001580"
     CDS             11894..12679
                     /locus_tag="VAH76_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HEO1386208.1"
                     /translation="MKPRIAVHKFTSCDGCQLAFLNAGESLLTLSDLVEIVHFAEAGM
                     VDVDMKVDIAFVEGSISTPDEEHRIRKIRENSQYLITIGACATAGGIQALRNFADSRE
                     WMASVYASPDYIKTLTTSTAIAHHVKVDWELWGCPVNSGQILEAIRFLLSNATPKIKR
                     DSECMECKRKGNVCVLVTKKQPCMGPVTQTGCGSLCPTLGRACYACYGPKENANPNSL
                     GEWFLSIGLTKEAIARQFLHINNQAPAFNQAGNYFKGIKISNG"
     gene            12672..13964
                     /locus_tag="VAH76_001581"
     CDS             12672..13964
                     /locus_tag="VAH76_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEO1386209.1"
                     /translation="MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLF
                     EKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGE
                     WLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
                     VGACVGGFYKAPSHPQIKLILEKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHP
                     TEYPMNEGRIVSDRGLNITKEEFDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNC
                     FGHLPAPIHLLLQSLNLQFPSRNMCHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPE
                     IKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLEHSLIQF
                     GLNQEEEAIRYFSEMIIRNYDPCISCSTHFLNLKIDRI"
     gene            13961..14440
                     /locus_tag="VAH76_001582"
     CDS             13961..14440
                     /locus_tag="VAH76_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEO1386210.1"
                     /translation="MNLIKILGIGSPFGDDQVGCLAADCLKTELSKDQYISKLVSIES
                     HDRPGLRLIELMNPAKVVFLIDAVKSGCALGTIHRLKNKEIYAFQNRLSTHEMGVAQA
                     LQLGHSLNALPEHIILYGIEIDNIAFNAMLSKPVEKAMRAVVIQIKKEIDEALKLMT"
     gene            complement(14500..15219)
                     /locus_tag="VAH76_001583"
     CDS             complement(14500..15219)
                     /locus_tag="VAH76_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEO1386211.1"
                     /translation="MVDYHYFKSKLNHFYGRYVGYNQRPVFFDIDQTFPELNHITRHF
                     PDIKKEFENVQANVKEFPRYHDIDPGESAISNTTEKNWNVFMLYLLGYQLKEARLLCP
                     TLCNLLEGIPHLIQAFFSILEPGKSIPLHEGPYIGYLRYHLGIHVPQNNPPQILVNNQ
                     PYTWKEGEAVLFDDSWPHEVRNTSDDYRAVLIIDVLRPMPFLPQLVNQFVTHIIARYF
                     YGRKVMKRAMQYGGEVKPLAL"
     gene            complement(15483..16415)
                     /locus_tag="VAH76_001584"
     CDS             complement(15483..16415)
                     /locus_tag="VAH76_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386212.1"
                     /translation="MSKQENIISHNTDSSRLNTVMQQMKERIINEGDKPHVTVETQLK
                     ILQELSQFGFGQFLIKNQGINGYWTHYMLTHPWFGRKTGLNNSREPLSNMECFLLDKA
                     PTMLATQQRFELFLQENQKALVNGATLASIPCGMLGELLYLDFNGINNIQLMGIDYDQ
                     AALEEANALAKQRSLDRFTTLVCRNAWELNIQEEFDLISSNGLNIYEPDDNKVLDLYH
                     QFHTALKKGGKLVTSFLTPPPHLSSQCEWMFNKINPEDLLLQRIIFSDILNAKFQCYR
                     SSQQTQKQLALAGFKNIHFLYDEAHLFPTVIAEK"
     gene            complement(16620..17258)
                     /locus_tag="VAH76_001585"
     CDS             complement(16620..17258)
                     /locus_tag="VAH76_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2461 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386213.1"
                     /translation="MDNRRKGMSQFNFFEKNQAFDENNFEQKLQDAINLIKQNQSFVA
                     RLVLDAINSNQVTLSTFFQLTPNHYQKTKTIMRKDYHTTLPNTFPPTISAIRKIEKEL
                     DGIIYDNQHIYISSTKSVEEIAKTLIHEVCHFLNADLYDKEMETEESKQVGYKDEVRA
                     FTAERMFEKNKGYLLRSDINQIHETVTELYPEFTDPENPISGYIYSTFDSPS"
BASE COUNT         4982 a         3025 c         3785 g         5492 t
ORIGIN      
        1 tgccaatatg ccgaaattat ttgttgttta tttgatcaaa tattaagatt tcttagcata
       61 ttttatgttt atgggttaaa ataaagagac ttttataaaa gttgacttca gtcatgcctt
      121 ttgcccatct cttgttagct ttattggttg ttttcgtttg gggaattaat tttattttcg
      181 tcaagcttag tcttgaagaa ttttctccac tgcttctttg cgctgtcaga tttcttttag
      241 ccagtgtccc ggctgtgttt tttatcaaac ctcctgctgt accttttaaa attattgctg
      301 gttatgggtt aatcatgttt gccttgcaat ttgcattgct ttttattggt ttgcgtgtgg
      361 gcatgactcc aggagtggct tctttgctca tgcaagtgca agtctttttc agtatgtttt
      421 ttgcaataat atttttagga gaacaacccc aagttgggca gataataggg gctttgattg
      481 catttatagg cattggtgta gtggccttac acttcgacca caatgtgtct ttaatggggt
      541 ttttatgtat tcttgctgca gcggcttcct ggggtattgg caacctcatt acgaaaaaaa
      601 ttcattctgt taatctgatt gctgtaattg tttggagtag ttttgttgcc tgtttgccca
      661 tgtttatact ttctcttgtt tttgaaggac ctgaaagctt tgtaactgcc tatgaacata
      721 tgacctggaa aggtattttt tccgtgcttt atattgttta tatttcaact tgggttggtt
      781 atggggtatg gaattggttg ataagccgtt atccagttgg catggtagta cctttcactt
      841 tattggttcc agttgtgggt attttgagtt ccgtctttat tttgggagaa ccattttatc
      901 tctggaagct ggttgccgga ttgctggtga ttagtggact ttgtattaat ttactgacaa
      961 ctcgcctgtt tgttgcaaaa acgcaacaac ctgagacagc ctagttatcc tcagctgatt
     1021 ctatatatcc ttttaagatc ataagtggag cagggggtta aatttctatc acccctataa
     1081 tatggattat cagataatta ttttggttat tacaaaataa ggataggggc agtaatatat
     1141 tacaattttt tttggaatta gaagttatta atgagtgatc tgaaaaatat attcttgtgc
     1201 gctgatgatt ttggccttaa tcatggtatt acccagggta ttttaaaaat ggctcgttta
     1261 cagcgcttat ccgcagtaag ctgtattgtg aatgccagtt cttttgatgt cgatgcccca
     1321 gacttattgg ctttgaaaaa tggggttcgc actggcttgc attttaatct cacggaaggt
     1381 tattttctat ctcatcctga caagctttgt tttggtttaa gagaattact catcagatcg
     1441 catttgggta tcttgaattc aagactaatc tcagatgaat tgaatactca attggatcat
     1501 ttcataaaag tcatgggaaa gttgccggat tttattgatg gtcatcaaca tatccatcag
     1561 tttcctggta ttaggcgaat tattattgat atgtatgaac agcgattaaa gggaaagagt
     1621 atttttattc gttccactta cccttcggtt aacatgtcag gatatcaagc aaaaacaaaa
     1681 attctgggaa taactggcgg taaaatttta agtagccagt taaagaaaac aaatattcct
     1741 cataatcctt attttgcagg gatttacgat ttttctgaca gaacaaacta cagatcttta
     1801 ttcaggcaat ggttaaacaa tgttccgaca aatacgttaa tcatgtgcca tcctggtgaa
     1861 ggtgagaata gtgatattat tgcagaggca agagccaggg aactaaatta ttttttgagt
     1921 gatgaattta tgcaagattg tgaggaatac caagtcaatt taacgacagg cccaaaatct
     1981 tgaaaaaata tagttgtaaa ctgcaacgat acttaaaaca aaaaccaggg cagcaatata
     2041 atgcaacgtt gtgaagcgta atagcagata atatagtgtt tcattaagta ggccggcaga
     2101 agctgccacc agaaaaaaat gaggtaaact taattgtttt tcatcttgta gctgattaaa
     2161 agtgaaatat ttgtgaccta aatagctgac attgaatgca attaaaaaag caatagcatt
     2221 agacagcagg ggttgaagat ttagaaaatt cactaaattg aacacgatca aaagatgtac
     2281 gacagcagcg cagagcccta ctccagtaaa ataaattaat cgatgggata atttaagagc
     2341 agtggtcatc aaaactggac tcctcatcga taatgtagtg gggtctgtgt ttcacttcat
     2401 caaaaacacg acctatgtat tcgccaacta cacctatagc aaataattgc aatccgccaa
     2461 aaaagctgat tgcagtgact agggttgccc agccaggggt ttcaatgcca aagattagcg
     2521 tgcttaatac aatccaggcg gcataaataa gcgccagggc tgctaccccc attcctccca
     2581 gggaaatcat tcttagagga aaagcagtaa acgatgttat gccagtaatt gccaaatcca
     2641 gtaaagaata aaaactccat tgcgaactac ccgatttacg ggggtgtaca ttaaatggca
     2701 cggctatttg tttaaaaccg acccagctgt ataaaccctt cataaagcga ttacgttcgg
     2761 gaagggtttg tagtgcatta acaatttttc tatcgagcaa tctaaagtca cctgcatttg
     2821 ccgggatatt aatacggttt attttggaag tgagatgata aaaaatttta gcgcaaactc
     2881 gttttagcca tgattcgtca gaacgatttt gtctcacggc ataaaccatg tcataaccgt
     2941 cttcccattt ggcgataaat tcgcttaata attcaagcgg gtgttggaaa tcactatcca
     3001 ataagatcac agcatctccg cgtgcatgat ccaatcctgc actgagcgct ttttccttgc
     3061 cgaaatttct actgaaacga atacaacgaa gagaatattc ttttcttaat gtttgaatga
     3121 ttgcgaaagt attatcttgg cttccatcat ctatcagtag aatttcatag ggataagaga
     3181 tgctcttaag agttttatcc aatgcggcga tgaattcagc aataagtact tcctcgttaa
     3241 aaacaggaac aacacaagag ataagttttt tatgttttgc agttttagac ataatgagta
     3301 ttaatgtacc agacaaaaga tagatagctt catatgttaa cctactcttg gaagttgagt
     3361 ccattgaggg ttataaatac agtgaaagta ttgatatatt gtccacagtc tttaacatta
     3421 ataatggcct tatactaaat gaatgattca ggttggtttt tgatttcaac tgctcttttg
     3481 cttggcatgc gtcatggttt tgatttggat catctggcaa cgatcgatgc gattagtcgc
     3541 acggttcgtt ataaaccttc tttatcgcgc atggttggct ttttgttttc actaggccat
     3601 ggtttggtag ttattattgt gagtttgatc attggcggag gtattatttc agcgagtact
     3661 cctgaatggt taaatgaagt gggaaatatt gtatcagtta cctgtttatt gttatttggg
     3721 tttttaactt tgtggaatac ggttcattat tcatcttctg ccatcatacc aaccaactta
     3781 aggagttatt tattcaggaa gcttgctgat aacaatgtac atcctcttat gattcttctc
     3841 attggcgcgc tgtttgcctt atcttttgat acgattagtc aaattgtatt gttttcactt
     3901 gcagccagtg ctttatcagg gtttctattt tccgggttat taggttttgt ttttatgctg
     3961 ggaatgatga tgtcggatgg attaaatgga ttttttgtag caagcttgat tcggcgtgct
     4021 gatgggatct cattactttt ctcaagatta gtaggatttg gcattgcttt ttttagcata
     4081 ggtattgcta taatcaattg ctgcaaattg ctttaaacag gctaaaaatg catgaattat
     4141 ggttatgtaa gcgtattgtc gagattatca agcaacaagc cactggcaat aaatgccgaa
     4201 tggtaaagaa aatagtgctg gaaatagggc agctagtagc agtcgataag cacgcattga
     4261 attttagctt taaagtgata actcaaggga cgattgcaca aaatgcagaa ttatcaattg
     4321 tagaaatccc aggtgaagca atatgcaatt cttgtcaaca aattgtacca atgaaacagt
     4381 attatgatga atgcctggtt tgcggcaatc attccttaac gctgacaaaa ggtgaagaat
     4441 taaaagtgaa atcaatggtg gtggaataat gtgcgggata tgcggatgtt cagtagaaaa
     4501 aggtcaaacc atggataatg agcaccatgc tactcaccat gagcattctg tgcctcatga
     4561 acattcaatt gatatcgaac agaatatttt agcgcaaaac aatgaatttg ctgctcataa
     4621 ccgggattat tttctcaggc ataaaatttt ggttttgaat ttgatgtcaa gtcctggatc
     4681 cgggaagaca acattgcttt ccaaaaccat ttcagatctt ggcaaggaaa tggatattgc
     4741 agtcatagta ggtgatcaac aaacaaattg tgatgccgaa atgctaaaat ccagtggagg
     4801 gaatgcctgg caaattaata ctggaaaagt ctgccatttg gatgcccata tggtgagtca
     4861 tgccatacaa gatatttctc taaaaaagca atcgctttta tttatcgaaa atgtgggtaa
     4921 tctcgtttgt ccggcttcat ttcatctggg cgagcaattc agaatagtga ctctttctgt
     4981 gacagaagga gagaataaac cgctcaaata ccctgatatg tttcattcag cagatttgct
     5041 cctgataacc aaagcagatt tattacccca tgtgaatttt gatttggatt tatgtaagga
     5101 atatgtacgc cgcacgaatc catccataga cattattacc ctgtcagcgg ttaatggtgt
     5161 tggattgaag gattggtacg attggttgtt gcattctttt aggttgaatg tccaggcttc
     5221 ttcatagaaa aagtggtagc ccatggaatt ggaacgggta caaatcagca ttcaaggact
     5281 agtgcaaggg gttggtttta gaccgtgcgt ttataagctt gccaaacaaa tggaattaac
     5341 tggctttgta caaaataatg catcaggtgt tttgatagaa attcaaggca actatttatc
     5401 acaatttatt ccgaaattaa gagagcaatt accgcctctc gcagcaattc ataacctaga
     5461 gttcacttca ctgccaattg ttccgaacga aagagctttt gaaattcttg atagccagca
     5521 gggtaaaata aatactgtca ttactccgga tgtttgcatt tgttctgaat gcctcggcga
     5581 attatttgac cctcaaagca gatactatcg ttatcctttc ttaaactgta ctcactgtgg
     5641 tccaaggttc acaattacca gaaatctgcc atatgatcgt tgtcaaacat caatgagcca
     5701 atttcccctt tgtcaatcgt gtcaatccga ctatttaaat cctgataacc gtcgttatca
     5761 cgcgcaacct acagcctgta gtcaatgtgg cccgcaactc gcgctatcag tcgacaaggt
     5821 agctcaaaga attcgggatg gagaaattat tgctttaaag ggtttgggag gatatcaatt
     5881 aatttgtgat gctcgaaatg agggtgctat ttcacaattg cggttaagaa aaagccgtga
     5941 ggccaaacca ttcgctttga tggtagttaa tgcgaaaagt gcagaaaaaa ttgccgaagt
     6001 aagcccccag gccagattgg tattggaaag cagagaaagg ccaattgtct tgttaagaaa
     6061 aaagaaagcg cttttaccgg atgggattgc tccgggctta aatcatttcg gtatcatgct
     6121 accttctact ccattacatt atttgctttt taattcgttc cttggcaacc cggattattg
     6181 ccaatggctg gatgagtatc attccatggt attagtggtt accagtgcga acataggcgg
     6241 tgaaccgctc attattgatg ataagaaggc cgagcaaggt ttaatttcaa tagccgataa
     6301 aattatctct catgatcggg aaatcattac tcgtacagat gactccgttg ttcgcatggt
     6361 taatgacact tcagtattga ttcgtcgggc acgcggctat gtgcctgctc ctttaagatt
     6421 gccttatgaa attcctccta ctgtggcctt gggaggacat cttaaaaata ccttctgtat
     6481 tactcgtggc gaagaagctt tcgtttcaca acatattggc agtttaaaca ataaagcgac
     6541 gattgagtat tttcatgagt cattgaacca tttattaaaa tttcttgcgg tcaaaccaga
     6601 acgaatcgct catgattggc atcctgattt ttataccagt agattggcgc aaaagtatga
     6661 tatacctgtt tacggggtgc agcatcatca tgcccacttg gcttctgttg tagcagaaca
     6721 tggaattcag gagcctgtat taggattagt tctggatggc tatgggtttg gttctgatgg
     6781 cgaagcttgg gggggcgaat tattattgct agagaaagcg gaatttaagc gcgttggaca
     6841 ttttttccct attcctcagc ccggaggaga acttgctgca agagaaccct ggcgtatggc
     6901 tgcgggcatt ttgcatattt tgggaagagg ggatgagatt tcacaacggt tcaaagagca
     6961 gccacaatca ccatttttac tgcaattatt agaaaaaaag atccattgtc cgctgactac
     7021 cagttgtggg cgactgttcg atgcggtcag cgccctttta ggagttgagt tcctgtctcg
     7081 ttttgaaggt catgcggcca tgcggttgga aagcatggtg actcatccac aagtcttaaa
     7141 caaaggctgg acgataaacg acaatgtttt tgacatgcgg cctactttac agtttatagc
     7201 aaacacgaca gagcaacagt taggggccaa tctgtttcat ggtactttaa tagcaggtct
     7261 tagcgagtgg gttacaggaa tttgtcgaga aaaagggatt cacatgctac tattgagtgg
     7321 cggctgtttt ttaaaccagg ttcttgcgga gggcttaatt aattcattaa ttaaatcagg
     7381 tattaaaccc ctgttgccca aattgcttcc accaaatgat ggtggaattg cgctaggtca
     7441 agcatggatt gttggaataa aagaataagg gtggcttatg tgcttagcgt tgccagcgca
     7501 aattgatcag gttttggaag ataacaaggc aatcgttaac ttgggtggga tcaccaagga
     7561 aatttccact gttcttttag agcaggtttc tgaaggggag tacgtcatta ttcatgcagg
     7621 attcgcctta acaaaactgg atgaaagaga ggcaaacaaa acattgagtt tgtttgccca
     7681 tatgctaaag gatggcttga agtgaaatac atagaagagt ttcgtaacgg tgcttacgca
     7741 caagccatta tcaatgaaat tatcgcaaca gcagatccac aaagacatta tcagtttatg
     7801 gagttttgtg gaggccatac tcatgctatt catcgttatg ggattccaag tttgcttccc
     7861 aacaatgttg aaatgattca tggccccggt tgccctgttt gcgttttgcc tatagcgaga
     7921 attgataagg ctattgcgct tgcctcaatg cagcaagtga ttctgtgcag ctatgctgac
     7981 atgctgcgag tcccaggaag tggacaaaaa agtttattaa ccgccaaagc atgcggcgct
     8041 gatgtcagga tggtctattc tgcgaatgat acattaaaaa tcgctcagga aaaccccaat
     8101 aaagacattg tgttttttgc tataggcttt gaaacaacaa tacctcccac tgcttttgtc
     8161 attaagcaag ctaaaaaatt aaacatcact aatttcagtg tattttgtaa ccatgtgtta
     8221 actcccattg ctatggatag tttgctacag gccaatgaag ttgccctgga cggctttgtt
     8281 ggcccagccc atgtcagtat cgtgattggg agttttcctt atgagcaagt ttgtaaaaaa
     8341 taccaaaaac ctatcgtgat atcagggttt gagccgttgg atgtgttgca ttctattctg
     8401 atgttaattc aacaattgaa tgaggggcgt tgtgatgtgg aaattcaata cactcgtgca
     8461 gtgaatcgta agggtaatct tttgtcacaa cacataatgg ccgaagtcct ggaaatacgc
     8521 gatcgttttg aatggcgtgg tttgggtttt attccatcca gcgcgttaaa gatcaaagag
     8581 tctttttctg agtttgatgc tgaacagcgt ttcgaattac ctgagagtgc agcgcaggaa
     8641 cataagcaat gtctttgtgg tgaggtatta aggggagtaa aaaaaccaac tgattgcaaa
     8701 ttatttgcca aggcttgctt gccagaaaat cctttaggat cctgtatggt ttcatcggag
     8761 ggagcttgtg cagcagttta tgcttatgga cgggttaatg ctttatgaat gaaatcctga
     8821 taactaaaaa attaacgggg ccaaagctga attttaaaga aggtactatc aatttgaccc
     8881 atggcagtgg tggacgtgct atggctcagc taattgaaca attattttta accgcatttg
     8941 ataatgaatg gttggcacag aaaaatgatc aggcttgctt tattgttcct tcaggacgta
     9001 tggtcatggc aaccgatgct cacgttattt ccccactttt ttttcctggc ggggacattg
     9061 gttctttgtc agtgcatgga actgttaatg atgtggctat gtcaggggca aaacctttgt
     9121 atttgacagc cagctttatt cttgaagagg ggtttcctct taacgatttg cgaaaaatcg
     9181 tttattccat ggctgatgca gccaggcaag caggtgttgc catcattgcc ggcgatacaa
     9241 aagttgtcga acgaggaaaa ggcgatggta tttttattac gacaaccgga gtagggattg
     9301 tccctgcggg aattcatatt tcgggcgatg cagcaaaacc cggtgacaaa atcattgtga
     9361 gtggttttat aggggaccat ggggtagcta ttatggcgca ccgcaataac ctggaatttc
     9421 agaccaaagt gcagtctgac acagcctctt tgcatgggct tgtcggtgag atggtgaaag
     9481 cagttccaga acttcattgc ttgcgtgatc caacgcgtgg agggcttgca acgacgctca
     9541 atgaattggc cttacaatcc aaagtcgggt ttgttattga tgaaagtaaa attccgattc
     9601 gtactgaggt tgccagtgcg tgcgaattat taggtcttga tcctctttat gttgctaatg
     9661 aaggaaaatt gatagcaatt tgccctcctg aaaaggcaga gaggctatta actgctatga
     9721 aagcgcacct cttgggttta catgctgaaa tcattggtga ggtgattgaa gatcatcgct
     9781 gttttgttca gctgaaaaca aaattgggcg ggttgcgaat tgtggattgg ctggccggag
     9841 agcaattgcc cagaatctgt taataagtaa taacgatcga caagaaagga cgaccaggat
     9901 gaacgacaag aggaactgtt ttttgcctca tgataaattg caacatttaa ttaattcttt
     9961 gcaaaatgct ggatactcct gtgtggggcc tcaggtgcgt gatggtgcga ttgtttatga
    10021 tgtcctgacc agggctgagc aattaccttg gggtatgcgt gatcatcaag aaccaggtgg
    10081 ctatcgattg gaacaaattt ccgagcgcaa agcttttgca tttgccaatg gccctcaggg
    10141 aataaagcct ctattgttca agccacagga aacagtttgg agggttgagc gtgatgaaca
    10201 aggaaaattg tgctttaaaa cacatcagcc agatgaaaca cctgttgcca ttatcggttc
    10261 tcgcgcttgt gatttagcgg cgatgagcat tcaagataaa gtttttatag aaagtaataa
    10321 cccggatcct cgttacaaaa gaaggcgcga gcaattattt gttgttgccg tgaattgtac
    10381 ctattcctct gcgaattgtt tttgtgtttc tgctggaact ggccctgcag tgaacaattc
    10441 ttttgatatt cttatgacgg aaattgaaga tggatttgtt gtggaagcag gtagtgataa
    10501 aggcaaaaag attattaatc aactggattt acatgatgca aaaagtgctc aatgtactga
    10561 tgcccaaaat aatgtgaaac aagccgctgc cttgcaaacc aagaggattc ctctggacaa
    10621 taaaagggat ttacgagatt tgctgttttc gaatttaaat catacccgtt gggatgaggt
    10681 ggccgaacga tgtttatcct gtggtaattg tactttggta tgcccaacct gtttttgtca
    10741 tagtgaagtt gaaaagccaa gcctcgatgg ttgtaccagt gaacatcata gagaatggga
    10801 ctcttgtttt acgacaggtc atacttatct caatgggaaa attattcgtg atgacacaaa
    10861 aaagcaatac aggcaatggt tgactcacaa ggttggaagc tggtttgatc aatttgatac
    10921 aagcggctgt gttggatgtg gacgttgtgt gacctggtgt cctgtgggaa ttgatataac
    10981 cgaagagttg gcagcaattt caggcgagtc caatgcaagg aaaatagaaa atgaataaag
    11041 tagtctatga tccttatttg ccaaaagaag ttgaaattat tcaacgtacg caggagtcgt
    11101 cttcaatttt tactctgcgc ttgcgcttta ctgatgaaga tcatcataag caatttcaat
    11161 tttatcctgg tcaattcaat atgctatacc tttatggggt cggtgaagtg gccatttcta
    11221 ttgtttcgga tcccagaaag aaaacttttc tgagtcacac tattcgagca gtagggcggg
    11281 taaccaaagc catgcaaaaa ctgcaggttg gtgatcgtct tggaatacga ggtccttttg
    11341 gagtaggctg gccactgcaa aaaacgatag gtaaagacat tattgttcta accgggggat
    11401 tgggttgtgc cccatctgtt tctattatca attatatttt aggaagacga cgtcattacg
    11461 gtaagctcag tattttgcag ggagtaaaac acagtgacga ttttattttt agaaagcagt
    11521 atgctaaatg gcaaaaatca gatcatactg aagtttatat agctgcagat caagctggtc
    11581 ctaaatggcc ttggggtgtt ggttatgtga ctgacttgat tgaccacatc atgatacaac
    11641 cagataactc cgtggtgatg atgtgtgggc ctgaaatgat gatgaataca gccgttaaag
    11701 tgtttaccca aaaggggatt cttgaaaacg acatctacct cagtatggag agaaacatgg
    11761 agtgtggaat tggtcattgt ggtcattgtc aatacggtgg tttattcgtg tgtaaagatg
    11821 gtcctgtatt tgcttattct caaattaaag agttattcaa agaatctgga ttttaaagtg
    11881 cctgaggaac gctatgaaac cgcgtatcgc tgtacataaa tttacttctt gtgatggatg
    11941 tcaattggca tttttaaatg cgggtgaatc gttgttaact ttatcagact tggttgaaat
    12001 tgtgcatttt gcagaggcag gtatggtgga tgtggatatg aaagtggata tcgcatttgt
    12061 ggaaggcagt atttcaactc ctgatgaaga gcatcgaata cgtaaaattc gtgaaaacag
    12121 tcaatacctt attaccatag gtgcttgcgc tacagcgggc ggtatccaag ccttacgtaa
    12181 ttttgcagac tccagggaat ggatggccag tgtttatgcg tcaccggatt atatcaagac
    12241 tttaactacc tcaacggcta ttgcgcatca cgttaaagtg gattgggagt tatgggggtg
    12301 tcctgtaaat agcgggcaga tattggaggc gattcgtttt ttattatcta acgcgactcc
    12361 caaaataaaa agagattctg aatgtatgga gtgcaaacga aaagggaatg tgtgtgtgct
    12421 ggtgaccaaa aaacaacctt gtatggggcc agtaactcaa acaggctgtg ggtcgctttg
    12481 ccccacttta ggcagagcat gttatgcttg ttatgggcca aaagaaaatg ctaatcccaa
    12541 ttctttggga gagtggtttt tatctatcgg attaaccaaa gaagcgattg ccaggcagtt
    12601 tttacatatt aataatcagg cacctgcatt taatcaagcc ggaaattact ttaagggaat
    12661 taaaatcagc aatgggtaaa gatatttcaa ttaatgttcc aattttggcg cgggtggagg
    12721 gagagggggc tcttgaactt tgcattcggg atagcaaaat tgaaacatta aaattaaaaa
    12781 tttatgaacc cccaagattg tttgagaaat ttctggaggg tagaagttac aatgaagtgt
    12841 tggattttgt agcccgtatt tgtggtatat gtcccgttgc ttatcaaatg agtgctaccc
    12901 aggctataga gcaatgtttt aacctaagtc ccactccttg ggttcgcgat atgcgtcgcc
    12961 tgttttattg tggcgaatgg ctggaaagtc atagcttgca cgtctatttt ttggctttac
    13021 ctgatttttt aggatttcaa tcagcacctg aaatggcaag gaaatatcca gaggaagtga
    13081 aatgcgggtt gcggattcag gcttttggta atgatttgat taaattgtta ggggggcgtt
    13141 ccgttcatcc agtgggagct tgcgtaggtg gtttttataa agcgccttcc catccgcaaa
    13201 tcaaattaat attagaaaag gcaaaagaac ggatctcaga ttgcgaagcg ttaattcatt
    13261 ggcttgcgag cttatcctta cccgataatt ctcatgattt taccagtgtt tcgctttatc
    13321 accctactga atatcctatg aatgagggaa ggattgtttc agatcgtgga ttaaatataa
    13381 ccaaagaaga atttgatgct tatttcaaag agcatcatgt ggattactcc aatgcattgc
    13441 actgtttatt gcaaaataaa ccttatttag taggtcctct ggctcgtatt aataattgtt
    13501 ttgggcattt gccagcaccc attcatcttc ttttacaaag cttgaatctt caatttcctt
    13561 cgcggaatat gtgccatagt attattgctc gagcagtgga gatgtactac tgtgttttgg
    13621 aagctatccg cattctggaa caatatgact tgccagagca atctcgtcct gaaatcaaac
    13681 ctcaggcagg agaaggagca ggttgtaccg aggctcccag aggtatgctg tggcagcatt
    13741 atgtttttga tgaaaccggt caggttttac aagcgcgtat tgttcctcca accagccaaa
    13801 atcaagcgcg tattgaggaa gatttagagc attctttaat acaatttggt cttaatcagg
    13861 aggaagaagc aatcaggtat tttagtgaga tgatcattcg taattatgat ccgtgtattt
    13921 catgctctac acattttctg aatttaaaaa tcgatagaat atgaatctta taaaaatatt
    13981 aggcataggc tcaccttttg gtgatgatca agtgggatgt ttggctgccg attgtcttaa
    14041 gacagaattg tccaaagatc aatacattag taaactggtg agcatagagt ctcacgatcg
    14101 acctggtctt cgtttgattg aattgatgaa tccagccaaa gtcgtttttt taattgatgc
    14161 tgtaaagtcc ggatgcgccc taggtacaat ccatcgctta aaaaataaag agatttatgc
    14221 atttcaaaac aggttatcga cccatgaaat gggggtggct caagctcttc aattaggcca
    14281 tagccttaat gctctgccag aacatattat tttatatggg attgaaattg ataacatcgc
    14341 ttttaatgcg atgctgtcta aaccagttga aaaagcaatg agagcagttg ttatccaaat
    14401 aaaaaaagag attgatgaag cactaaagct tatgacataa gtttctttat gccttaggcg
    14461 gtcttctcaa ttgatatctt aaagaaatcg tttcttaatt taaagcgcca aaggttttac
    14521 ttctccacca tactgcatcg cgcgtttcat tactttgcgt ccataaaaat atcgagcaat
    14581 gatgtgggtt acaaattgat tgaccagttg cggcaagaaa ggcatagggc gcaagacatc
    14641 aatgattaat acggcacgat aatcatcact tgtatttctt acttcatgtg gccaggagtc
    14701 atcaaaaagt actgcttctc cttctttcca ggtataaggt tggttattaa ctaaaatttg
    14761 tggtggattg ttttggggaa catgaattcc taaatgatat cttagatagc caatataggg
    14821 gccttcatgg agggggattg attttccagg ttctaaaatc gagaaaaaag cttgtataag
    14881 atggggtatt ccttccaata aattacaaag tgtcgggcat aataaacgcg cttccttgag
    14941 ttgataacca agaagataca gcatgaaaac attccagttt ttttctgtgg tatttgatat
    15001 ggcagactct ccgggatcta tgtcgtggta gcgtggaaat tctttcacat tcgcttgtac
    15061 attttcgaat tcctttttga tatcaggaaa atggcgtgtt atgtgattta attctgggaa
    15121 agtttgatca atatcaaaaa aaaccgggcg ttgattatat cctacatagc gcccataaaa
    15181 gtgattcaat ttactcttga aataatgata gtctaccatt gcttattcct ttattttatt
    15241 tgtgataata atcaggtgcc gaatgttcga ctatccttgg gaaaatcagc tattttaaaa
    15301 tgaagtttga agaaatggaa gtttaaaaaa tgctcgtttt tgtgccgagg tatcctttca
    15361 ggttttggaa atgattcttg gcatccagct tattccaagt aagcatattc tcaatggcag
    15421 tcatcatata acatttatcg ctatggctta aaaaaatacg gataacaatt tccattttct
    15481 tgttattttt cagctattac agttgggaag agatgggcct cgtcatatag gaaatgaata
    15541 ttcttgaagc ctgccaaggc gagttgcttc tgggtttgtt gcgatgagcg ataacactgg
    15601 aattttgcat ttaaaatatc ggaaaaaata attctctgca gcaataaatc ctctggatta
    15661 attttattga acatccattc gcattgtgaa ctcagatgag ggggaggagt taaaaaactg
    15721 gttactaact tcccgccttt ttttaaggcc gtatgaaact gatggtataa atccagaact
    15781 ttgttatcgt ctggttcata aatatttaac ccattgctgg aaatgagatc aaattcctct
    15841 tgaatattaa gctcccaggc attacgacaa acaagcgttg tgaatctgtc caggcttcgt
    15901 tgttttgcca aggcattggc ctcctctaat gctgcctggt cataatctat acccatcagt
    15961 tggatgttgt tgatgccatt gaaatcaaga taaagtaatt cccctaacat cccacagggg
    16021 atagaagcaa gggttgcgcc attaaccaga gctttttgat tttcctgcaa aaataattcg
    16081 aaacgttgtt gggttgctaa cattgttggt gctttgtcca acaaaaaaca ttccatgttt
    16141 gataatggct cgcgactatt gtttaaccct gtctttcttc caaaccaagg atgagtcaac
    16201 atataatgag tccaataacc attgataccc tgatttttta ttagaaattg accaaaccca
    16261 aattgactta attcttgaag aatcttaagt tgagtttcta ccgtgacatg tggcttatca
    16321 ccctcgttta ttattctttc cttcatttgc tgcattacag tattcagacg agatgaatcg
    16381 gtgttatgag aaatgatatt ttcttgtttt gacatgcagg ctctctggat aatcttttaa
    16441 ttattaattg ctgtgagttt aagataagaa aatggattta ttttataatt caaactcaca
    16501 acaattatac ttaatattct tgaagatacg tagatttttt aactcatttc attgtacttc
    16561 cgctacggcg ctcgaaaatc gaggcttttc gactcaagct ggcaaaatgt aaacagccct
    16621 taagaagggc tatcaaaagt gctgtaaata taccctgata ttgggttttc cggatcggtg
    16681 aattcaggat atagttcggt aaccgtttcg tgtatttggt ttatatcact tctgagcagg
    16741 tagcctttat ttttttcaaa cattctttct gctgtaaatg cccttacttc atctttatag
    16801 ccgacttgtt ttgactcttc tgtttccatt tctttatcgt aaagatctgc atttaaaaaa
    16861 tgacagactt catggattaa tgttttggca atttcttcaa cgctttttgt cgaactgata
    16921 taaatatgtt gattatcata aataatgcca tccagttctt tctcaatttt acgtattgcg
    16981 gaaattgtag gggggaaagt atttggaagg gtagtatgat agtcctttct cataattgtt
    17041 ttcgttttct ggtaatgatt tggggtaagt tgaaagaaag tacttaatgt gacttgattg
    17101 gaatttatcg catcgagcac taatcttgca acaaaagatt gattttgttt tatcagattg
    17161 attgcatcct gcaatttttg ttcgaaattg ttttcatcaa aagcctggtt tttttcaaaa
    17221 aaattgaatt gagacattcc ttttctccta ttatccagta ttaatgatta cttccatgca
    17281 attt
//
LOCUS       DATNSN010000059        17159 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.059, whole genome shotgun sequence.
ACCESSION   DATNSN010000059 DATNSN010000000
VERSION     DATNSN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17159
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.059"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1307)
                     /gene="bioA"
                     /locus_tag="VAH76_001586"
     CDS             complement(<1..1307)
                     /gene="bioA"
                     /locus_tag="VAH76_001586"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEO1386214.1"
                     /translation="MVNTDQLIAKDLKHIWHPCSQMKDFETFPPIVVNKAQGSYLYTN
                     KGPVIDAISSWWCKSLGHGHPAVIAAIKNQLDCFEHVISANTTHPAAVELAEKLSEIT
                     GKQHVFFASDGSSAVEIAMKLAIHASQIKGHKNKNQFIALKNGYHGETIGTMSVSDLG
                     LYKAPYASFGVQCHYIDDIPYVSGFKDPLWTDCDAYWNKVVQQLNKICDKVCAILLEP
                     IIQGAGGMLCYSADFLSKISSWAKEKGIYLIADEIMTGIGRTGEWLACDHARVQPDLI
                     CLSKGLTSGSIPFSCVMIDSTLFDLFYDDYHTGKSFLHSHTYSGNPLAIVAALATIKT
                     IEQDNLLMHSQELGKIMFSYLQDIARDSRKLVNVRGVGAVVAADLIEGSQARIGFKIY
                     QEAIKQGALIRPIGNTLYWLPPLNTDKKIIGKLAEITLNSIEEA"
     gene            1380..2327
                     /gene="bioB"
                     /locus_tag="VAH76_001587"
     CDS             1380..2327
                     /gene="bioB"
                     /locus_tag="VAH76_001587"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO1386215.1"
                     /translation="MSEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFA
                     TLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAA
                     WRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
                     YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPE
                     SVPINRLIPVEGTPLAQAERVEGIELVRTIATARILMPKSAIRLTAGRTEMSDELQAL
                     CYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMA"
     gene            2328..3473
                     /locus_tag="VAH76_001588"
     CDS             2328..3473
                     /locus_tag="VAH76_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEO1386216.1"
                     /translation="MTLNIKINEYTAQLQEQGLLRTRQILETDKIDYIRFDSNDYLSL
                     IEDRRIAEAYQKGYELYPCGSGASMVLSGYHSSHQTLERAFAEMLGVDDCLLFSSGYC
                     ANLAITALLGRLGTHCFIDKSVHASIYDGLALSRVTYSRYIHNDMNALSALLKSHLGD
                     SVLITEGIFSMSGQIAPLSTISTLCKENGSELFVDEAHAFGVIGPQGMGSVPSHGLTQ
                     NEVPLRVIPFGKAFVSQGAVVGGQKDWINALLQTARSFVYSTAISPALCYGLLKTLEI
                     ITAADSRRAKLNQLVAQFKELIKSSPLNWLDSDTPIQQLRLGCPHKALHYASELKKDG
                     IYCSAIRSPTVNSASSGLRIIINYKHTIEQIIELFNKLHLIHEYSSR"
     gene            3454..4173
                     /locus_tag="VAH76_001589"
     CDS             3454..4173
                     /locus_tag="VAH76_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1386217.1"
                     /translation="MNIHLDKYGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVD
                     LPGFGLTPMMDWESFKKNLLDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITS
                     SPRFISDVDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
                     LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRA
                     AHMPFLSHTDLFITMMDEFIK"
     gene            4170..4808
                     /gene="bioD"
                     /locus_tag="VAH76_001590"
     CDS             4170..4808
                     /gene="bioD"
                     /locus_tag="VAH76_001590"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEO1386218.1"
                     /translation="MKRYFVTGTDTDCGKTFVTNQLVNYFSSSAAIKPIASGCEYSKN
                     QLVNSDALLHQQQNHLPLEIINPWRFRLPVSPHLSAREDGASIDVHKVADYCLNLQLN
                     DIKKLFIEGAGGLMVPLNEQDTWLDFLKLTRIPVILVVGLKLGCINHTLLTQEVLEIN
                     KIKCQGWIANCLDQDMLMLDENISTLEAKLKYPLLARTNYGGKISDICLSSL"
     gene            4943..5257
                     /locus_tag="VAH76_001591"
     CDS             4943..5257
                     /locus_tag="VAH76_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEO1386219.1"
                     /translation="MPIYEYQCTNCHHQFDLMQKVTDAPVKQCPKCYENTVTKLISPA
                     GFQLKGTGWYVTDFKNKGNGPKESTENKSGDPGINKESSSKETPAASSADSAKSTKGD
                     SE"
     gene            5254..5883
                     /locus_tag="VAH76_001592"
     CDS             5254..5883
                     /locus_tag="VAH76_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEO1386220.1"
                     /translation="MKLISLRAYLITGLIVWLPIFVTIVVLRFIIDMLDSTLALFPKA
                     YQPEQLFGFYIPGFGVLFSLVLLLVTGIIATNFFGQRLVSRGESLLAKIPLVRSIYNA
                     VKQVIHAVLSTNSQAFRKVVLVEYPRKGLWTIAFQTGSVNPEIKEKSKEDMMSVFVPT
                     TPNPTSGFMLMIPRQDAIELNMSIDEALKLVISLGVMQSDPALAQVKTQ"
     gene            5902..7683
                     /gene="aspS"
                     /locus_tag="VAH76_001593"
     CDS             5902..7683
                     /gene="aspS"
                     /locus_tag="VAH76_001593"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO1386221.1"
                     /translation="MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGL
                     LQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEIL
                     NQSPTPPFLPDDHQIINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
                     DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCF
                     RDEDLRADRQPEFTQLDIEMAFINEEDILQLIEGLLKIVFKEILNITLPDKLPRMSYK
                     EAMTRYGSDKPDLRNPLELIDIADLVKDCDFNVFSSAANDNSGRVVALKLPNGCDLSR
                     KDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEAQTG
                     DVIFFGADKAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPM
                     HHPFTSPQELSAEALRSKPTQTLAKAYDIVINGYEIGGGSIRIHQPELQKTVFDLIGI
                     GEQEAQEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKTQTASCP
                     LTSAPSPAGNAQLTELGIRLAPTITTK"
     gene            7658..10609
                     /locus_tag="VAH76_001594"
     CDS             7658..10609
                     /locus_tag="VAH76_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEO1386222.1"
                     /translation="MRQLLRQSNLNLSTTSLKYLWCFFLPLFLLFFSFSLFSASVKEN
                     NATLVEYLAHEKTHLTIAINEIKQKSAPKNEQEYNEKLRQIRTILSMNKAKIESLNGF
                     SENQKKEEVNLNEQLKRLQQMPLANAEVTIQEKVGKIETLLTVNKKTTELIAETLDLA
                     KQYQLLLNDVLNELKLWKANFDLKEKLKSIKAIRAKLNKELNILYENAVSSQDKKQPE
                     LTISEQIQYEANLYINNQRIAVIHQKLNALELQKKIIKTNILLLNNLDTKALQSAIDN
                     YREVINQYSAIESSLQTILNSLTNQANLFVEPGLQKSVSLLQNDVKTELKETSNQKLI
                     LSKNLEDYQNQLKKQISTRQSLSEYNIDSWAVILKKLIDIPNLFYKYIKTLTIKVYDS
                     YTWLDTFPAIFLWTVLIFILSLFYILNHVLKTLARDKERSRFTGYLYGGILVLLQRNI
                     PHLCFFAMLSFILYFTSISYANSQLLIKLIIVWLTFRVLILIARLILLESISDSSGKD
                     VKLYYRLKWLLLFGGWTTALMTFSHLLPLSILLQDIFNRLFMLFMLTVSLVAWKSKDV
                     IPYLIRPLLKSKKRYFKNAISLLVILVPITLFTTAIIGLIGFVNLAWTMSRYQAYILL
                     VLVGYILVRGLIFDALELFSEWMISSLKNGWLWIEVFLKPIDKILRIVLFLTSVLILF
                     QLFGWYSDSLVVTSLEKVVQYSILNITGIHITVASIIEFLILLAVLVWAAKWTREFCF
                     RWLYKNTKDVGIRHSLSVFTQYAIILLGTFITLHVLGLDFSGMSMILGGLAVGMGFGL
                     RDFASNIVGGIMLLIERPVREGDLITIGEYEGQVAHIGIRSMRVSSWDNTQVLIPNAE
                     TFNKPFTNWTHQDGIIRTVIPIKVSRADDPVMIQQLILDVLAIIPEIVPDPPAQVFLK
                     KIDTALIEFEARYFINIQNHNRVEVRSKVLFAITAQFKAAGIQPPIEPITVELKESSS
                     DFSLKKQTTEE"
     gene            10572..11246
                     /gene="mutH"
                     /locus_tag="VAH76_001595"
     CDS             10572..11246
                     /gene="mutH"
                     /locus_tag="VAH76_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HEO1386223.1"
                     /translation="MILALKSKPPKNEKELLEQCQRIEGLSFAQLALELGLFTPLNPV
                     HRKGWVGQALEIALGTDAKNESLPDFKQLGIELKTIPINKSGKPAESTFIVSIPLLTI
                     HQQSWESSQCYAKLKRVLWIPIEGDTDIPFPHRRIGRGFLWSPNKDQKSVLAEDWNYL
                     TNQIVLGQLETLNSSSGEYLQVRPKAANGKSLCYGYDPEGNRIRTLPRGFYLRSCFTS
                     VILSQS"
     gene            complement(11319..12359)
                     /locus_tag="VAH76_001596"
     CDS             complement(11319..12359)
                     /locus_tag="VAH76_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386224.1"
                     /translation="MKTIFANKELLYNQYKKMFLEQISDISKLKECVKARYDIKELPD
                     EIFFKLLINNHKKIYDFIGTHNQNRIRYFFGNFDNFDPRDNQISVLDRFILDSVHFYI
                     TSSLLMQLHKEYQENAIEIDKAWQKEIRAHAHLGPLPTTAIIANSMKSNKNHVFKDLQ
                     FPANKNIQLFQLNKLSIKLNDQVFALWLPKTSEAQVSLSNFTRNTIFVCIATAAILAI
                     GVSLFVFSASSVAGLAVIFNPASLGIMIALLACATLMLGISSLYSSTTIHDSTIEHPE
                     NRTPEEPYHSHNIRKDKMHSSKAKPEEQLPYHDPKWLLFNRKPYTLRESIVTPATASE
                     QTSSMHTNRQKL"
     gene            complement(12558..13394)
                     /locus_tag="VAH76_001597"
     CDS             complement(12558..13394)
                     /locus_tag="VAH76_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386225.1"
                     /translation="MGKVNAVGMAEVGVERAKRKEALRVLTSSPFNLDNKEHTQALEK
                     YIKQFELSVNQMYLYQTMSTALTAWGGSWIVGRFLPIPEFANHLLAACLYLGVSGYIL
                     ESFSMTDFYKQLKEMKVLYNWCLKNNKENYSTDIDNTNNLLYPDIQRMIKLIAPLCST
                     DFMIAWPKEVKTAETQNGILSNTLSIGYSIVSAPFSLFSTASKPSQDQVQRIKDLKVQ
                     VETRGLDLGVFAGFEQAVKYFATNSDFRELLKAKLTQPVNSFKNLLQTTIPGMLAGSN
                     SK"
     gene            13540..15129
                     /gene="legK1"
                     /locus_tag="VAH76_001598"
     CDS             13540..15129
                     /gene="legK1"
                     /locus_tag="VAH76_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector kinase
                     LegK1"
                     /protein_id="HEO1386226.1"
                     /translation="MPRTMFFSVDAANLQNLQITNRKAFAALESYLNDNDSPGAWNNN
                     QGYKYTHKDVTYCFSFNQSLVRRPRKSDQKKHTFEIFDPNKNPLGKGAYGIVYPILGA
                     IQFEFGTAIKRPPKNKLVKIQNHSERDQSHSVLREYQGLLQSGHIAVKPPIFVENKES
                     KFSYLIMEKAEGIVLEKFLNPVKRLDLSEEVPELNLINRIEITFAILKAIKEQVTDKH
                     LIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALY
                     TAKADVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKED
                     KSKIRSCLSAMLIVDPEERGSIDKAIKQFSQIDFEKYKRLKRSTNFEIDLTDNDIKLR
                     KQLNSIHLHLITLQGKEKDLRDRACFDAANAMSQLVSKLSTYTNYLEKNPDPFLIKRY
                     KECCIAEIDLANLALQNHRDALWLVAELSTAILLLGIGYLFAFGINYYYTGRLGLFSQ
                     TRSEKLVEEMKSSVLGIAVGN"
     gene            complement(15183..15992)
                     /locus_tag="VAH76_001599"
     CDS             complement(15183..15992)
                     /locus_tag="VAH76_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1484 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386227.1"
                     /translation="MPSREMNCCIGKSLNYMRFSMSSFKHSCEKIDINLAHNVLTICP
                     KNREALQRITALFNNLYILYFIGGIPSITYDDMDQIQINIKYSQQITDAKLKNTLLSF
                     VNSELISQDEKETFLNELELTKTSFTTQFNTELENIRKKAEELSKKAEVDPYHYESAS
                     KAANRLHQELLIASQLYFYHKTPEVYENFKQTCQREIERARPVLEQHRGWKPLLVNVG
                     AAIIGLGVLYLLAASINYYKTEGRHFFFHFETDSLQKLENLNMAQSQVLRI"
     gene            complement(16182..16583)
                     /locus_tag="VAH76_001600"
     CDS             complement(16182..16583)
                     /locus_tag="VAH76_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386228.1"
                     /translation="MQESIHSLFLAQAIGLYLLIVGIIMLSRASYYRELLTHLKEGSA
                     TIVTAGSLGLIIGICLVLVHNIWVPESEILVTLVAWLILIKSILWLGFPEFMVKCSQK
                     AYTGTGYYVISIVVLIIGILLITHGFYLFSH"
     gene            complement(16669..17130)
                     /locus_tag="VAH76_001601"
     CDS             complement(16669..17130)
                     /locus_tag="VAH76_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEO1386229.1"
                     /translation="MLDKIDRKILNILQKNNQITNQALADIVGISAPPCFRRVKRLRE
                     EKIIVNDVALVDPFKVGRPLIVFVNITLEKQREDLLAHFERKMQEEPEVMQCYFVSGD
                     TDYLLIIHVKDMNHYNEFARRVFANEPNIRKFRSSFCLNRTKYNTQVVLDE"
BASE COUNT         5373 a         2939 c         3337 g         5510 t
ORIGIN      
        1 gcttcttcta tagagtttaa tgtgatttct gctaatttcc caataatttt tttatctgtg
       61 ttaagaggtg gtaaccaata gagagtattt ccaattgggc gaatcagtgc gccttgtttt
      121 attgcttcct gataaatttt aaatccaatt cttgcttggc tgccctctat gaggtcagct
      181 gcaacaactg ctccaacacc acgaacattt acaagttttc tactgtccct tgctatatcc
      241 tgcagatagg aaaacatgat tttaccaagt tcctgactat gcatcagtaa attatcttgt
      301 tctattgttt taatagttgc taaagcagcg actatagcaa gtggattacc gctataggta
      361 tgtgagtgca aaaaagattt ccctgtatgg taatcatcat aaaaaagatc aaataaggta
      421 ctgtcaatca tgacacagct aaatggtatt gagccggaag ttaatccttt agatagacaa
      481 atcagatcag gttgtaccct tgcatgatca caggctagcc attctccagt tctgcctata
      541 ccagtcataa tctcatcagc aattaaataa atgccttttt ccttagccca tgacgagatt
      601 ttcgagagaa aatctgcact gtaacatagc atacctccag caccctgaat gataggctcc
      661 agaagtatcg cacaaacttt atcgcaaatt ttattcaatt gttgtacgac tttattccaa
      721 taagcatcgc aatctgtcca taagggatcc ttaaagcctg agacataggg aatatcatcg
      781 atgtaatggc attgcacgcc aaacgatgca tagggagcct tatagagtcc caaatcactg
      841 acactcatgg tgccaatagt ttctccgtgg tagccatttt ttaaggcaat gaattggttt
      901 ttatttttgt gcccttttat ttgtgaagca tgaatagcta atttcatggc aatttcaaca
      961 gcactggaac catcactggc aaaaaacaca tgctgttttc ctgtaatttc gcttaatttt
     1021 tcagctaatt ccactgcagc aggatgagtt gtattggcac taatcacatg ctcaaaacag
     1081 tccagttggt tcttgatagc agctatcact gctggatgac catgccctaa tgatttgcac
     1141 caccaactgg aaattgcatc aatcacgggg cccttattgg tatagagata gctaccttgt
     1201 gctttgttga caacgatagg ggggaatgtt tcgaaatctt tcatttgtga acaaggatgc
     1261 caaatgtgct ttaagtcttt tgcaataagt tgatctgtgt ttaccatttt aaaaattttg
     1321 gttgacaagt ataggcgcga ctttatcatg ttcactattc tgaattaaag ggggtagccg
     1381 tgtctgaaga aaataaacag tggtcaattt ctgatattga agcaatttat caacaaccat
     1441 ttaatgattt actttatcaa gcacatacca ttcataggac atatcacgat cctaattctt
     1501 tacaatttgc aactttgtta agcattaaaa cgggtgcatg cccagaagat tgtggctatt
     1561 gctctcaaag tggtcactat aaaacgcatg tagaaaaaga aaaattgatg tcagttgaag
     1621 aggtattgca atgtgccaaa gaagcaaagg agggtggggc caaacgtttt tgtatgggag
     1681 ctgcatggcg ttgtccacct gataaagcaa ttccgcagct aaaagaaatg atagaaggag
     1741 taaagtcttt agggcttgaa acctgtatga ctttaggcat gcttaccaaa gaacaggcct
     1801 ctcatttaaa ggaggctgga ttggattatt ataaccataa tattgatacc tcaccatcct
     1861 attacgataa ggtggtaacc actcgaaaat tcagtgatcg attggatact ttaaataacg
     1921 ttcgttcagc gggaattaat gtgtgttgtg gtggtatttt aggattgggg gaaaccagag
     1981 aagatcgaat tgaattttta ttaacattag ccaatatgga aactcctccg gaaagtgtgc
     2041 caattaatcg tttaattcca gtggaaggaa cacctttagc ccaagctgag agagtagaag
     2101 ggattgaatt ggtaagaacc attgctacag caagaatttt aatgcctaaa agtgctatta
     2161 gactgacagc cggtcgaacc gaaatgagcg acgagttaca agcactttgc tattttgctg
     2221 gagccaattc tgtgtttatt ggcgataagt tattgacaga agataatccc cagcgcttca
     2281 aagacaaaac tctttttaac aaactgggtt tgactgagat ggcataaatg actcttaata
     2341 taaaaattaa tgagtataca gcacagctgc aagaacaagg attattgcgt acaagacaaa
     2401 ttttagaaac ggataagata gattatattc gatttgactc taatgattat ttgtcgctca
     2461 tagaagatag gcgaattgct gaagcctatc aaaagggata tgagttgtat ccttgtggaa
     2521 gtggtgcttc gatggtttta agtggctacc atagtagcca ccaaaccctt gagagggcct
     2581 ttgccgaaat gctgggagta gatgattgtc ttttattttc ctctggctac tgtgcaaatt
     2641 tggccattac ggccttgctt gggcgtttgg gaacacattg ttttatagat aaaagcgttc
     2701 atgcatcaat ttatgatgga ttggctttgt cacgagtaac ctactctcgt tacatacata
     2761 atgacatgaa tgctttgtct gccttgttaa aatctcattt gggcgattct gttttgatta
     2821 cagaaggtat ttttagtatg agtgggcaga tagcgccgtt atcaacgatt agtacgttat
     2881 gcaaagagaa tggctctgag ctctttgtag atgaagcgca tgcttttggt gtgattggac
     2941 cgcaaggaat gggctctgtg ccaagccatg ggctgacaca gaatgaagtt cccttgcgag
     3001 ttattccttt tggtaaggca tttgtttccc agggcgctgt ggttggtggg caaaaagatt
     3061 ggataaatgc tttgttgcaa acggcacgtt cttttgttta ttcaactgct atcagtcctg
     3121 ctttatgcta tggtttgctc aaaacattgg agataataac agctgccgac tccagaagag
     3181 caaaattaaa ccaattagtt gctcaattta aagaattaat aaaatcttcg cccttaaatt
     3241 ggctggattc agatactcca attcagcaat taaggttagg atgccctcat aaggcactgc
     3301 attatgccag tgaactgaaa aaagatggga tttattgctc agctatcaga tctccaacgg
     3361 taaatagtgc ttcttccggt ttacggatta ttattaatta taagcatact attgaacaaa
     3421 tcattgagtt atttaataaa ttgcatttaa tacatgaata ttcatctcga taaatatggg
     3481 caaggtatgc ctttggttct atttcatggc tggggatttg atagccaaat ctggcaaccg
     3541 ataattcctt accttaagcc taaatatcag attattttgg tcgatttgcc tggttttgga
     3601 ttgactccaa tgatggactg ggagagtttt aaaaagaatt tgcttgacca attacctgat
     3661 aaatttgcat tggcagggtg gtcaatgggt gggctgtacg cgacacgttt agcaattgaa
     3721 gagcctgcca gagtgcagta tttgataaat ataacgtctt cacctcgctt tatctctgat
     3781 gttgattggc cgggagtagc agaggaggta tttgtcaatt tttataacaa tttatcgaaa
     3841 gatattaata agacattaaa agagttcata tccttgcaac tcaataagat gaaatttgat
     3901 tttaaaatag gtaatcctcc atctccagaa ggcttggcgt ttggtctcga aattttgggt
     3961 acctgggatt ttagagagca gttgaaacaa atatctatac ctactgttta cttatttggt
     4021 cgacttgatc cgattactcc tgccaagacc atggcaatta tggagaaaaa ttaccccaat
     4081 tttaagtatg tcctcttcaa cagagcagct cacatgcctt ttctgtccca taccgattta
     4141 tttattacaa tgatggatga atttataaaa tgaaacgata ttttgttacg ggtactgaca
     4201 ctgattgtgg taagacattt gtaaccaatc aattggttaa ttatttttca agttcagcag
     4261 cgataaaacc tatagcaagc ggatgtgagt acagtaaaaa tcaattagta aattctgatg
     4321 cacttttaca ccagcaacag aatcatttgc ctttggaaat tattaatccc tggcgattta
     4381 gattgccagt ttcgccgcac ttatcagcaa gggaggatgg tgcaagtatt gatgttcata
     4441 aagtggctga ttattgcctt aatttgcaac tgaatgatat taagaaatta tttatcgaag
     4501 gagccggggg attaatggtt cctctgaatg agcaggacac ttggcttgat tttttaaaat
     4561 taaccaggat ccctgttatt ttagttgtgg gcctgaagtt gggttgcata aaccacactt
     4621 tgcttaccca ggaagtgctc gaaataaata aaatcaaatg tcaaggatgg attgctaatt
     4681 gcctggatca ggacatgcta atgcttgatg aaaatattag tacccttgaa gccaaattaa
     4741 aatatccttt attagccagg acaaactatg gcggcaagat ttctgatata tgtttaagct
     4801 ccttgtaatc gcagtgaaat tttatatcaa aacagcacta tatacctgat gttattgctt
     4861 gttctgcttg cgctataccc atggcagcaa ttataatgaa aagttaaatt acagatactt
     4921 cgagtttatg gagtgatgct agatgccaat ttatgaatat cagtgcacaa actgtcatca
     4981 tcaatttgat ttgatgcaaa aagttacgga tgcgcctgta aaacaatgcc caaagtgtta
     5041 tgaaaatact gtaacaaaac ttatttctcc ggctggattt cagttaaagg ggactggctg
     5101 gtatgtcaca gattttaaaa acaaaggtaa cggaccaaag gagtcaactg agaataaatc
     5161 aggagatccc ggcatcaata aagagagttc ttctaaagaa actccagcag caagctctgc
     5221 agatagcgca aaatcgacaa aaggtgatag cgagtgaaat taatctccct cagggcctat
     5281 ttaattaccg ggttaatagt gtggttacca atatttgtaa ccattgtagt cctgcgattt
     5341 attattgata tgcttgatag cactttggct ttatttccaa aagcttatca acctgagcag
     5401 ttattcggtt tctatatacc aggctttggc gttttatttt cattggtttt attactcgtg
     5461 acaggaatta ttgcaaccaa tttttttggg cagagacttg taagtcgggg tgaatcctta
     5521 ttagctaaaa tacctctggt gcggtcaatt tataatgcag taaagcaagt tattcatgct
     5581 gttctatcaa ctaatagtca agcttttcgt aaagttgttt tagttgagta tcctcgtaaa
     5641 ggactttgga ccatcgcatt tcagactggc tccgttaatc ctgaaattaa agagaaatcc
     5701 aaggaagaca tgatgtctgt atttgttcca actacaccta accctacttc gggttttatg
     5761 ttgatgatac ccaggcaaga tgccatcgag ttgaatatga gcattgatga agcgttaaaa
     5821 ttagtaatat cgttaggtgt aatgcagtct gatcctgcat tggcacaagt aaaaactcaa
     5881 taaactttaa taggtggttt tatgcgtaca cactactgtt cggccgtgaa tgaattaagc
     5941 ttggataagc aaatcacagt ttgtgggtgg gtgcataata gacgagatca tggcggagtt
     6001 atttttttag acattcgtga tcgttccgga ttattgcaag ttgtgtatga gcctgaaaat
     6061 aaggaaattt ttgcaattgc tgaaaaacta agatcagagt ttgttgttcg tgttactggc
     6121 atagtccgta aaagaccaga aggaatgatc aatgataaaa tggagactgg cagggttgaa
     6181 gtaatcggta cacaacttga aattcttaat caatctccaa ctccaccgtt tttgccagat
     6241 gatcatcaaa ttatcaatga agatttgcgc tataaatacc gctatattga tttgcggcgt
     6301 gctgttatgc agaagaaact gactttaaga cacaaattaa acagttgcat acgtaattac
     6361 ttgaatgagc agaacttttt agatatagaa acgcctatgt taactaaggc aactcccgaa
     6421 ggcgcgcggg attatcttgt gccttcaagg gtacatccag ggcaattcta tgctttacct
     6481 caatcgccac aattatttaa gcaattgttg atgatgtcag gatttgataa atattaccag
     6541 attgtacgct gttttagaga cgaagattta agggctgaca gacagcctga atttacgcag
     6601 cttgatatag aaatggcatt tattaatgaa gaagatatat tgcaactaat tgaaggattg
     6661 ctcaagattg tatttaagga aatccttaac attactttac ctgataaatt gccaagaatg
     6721 tcgtataaag aggctatgac acgctatgga agtgataaac ctgatctgcg taatccactg
     6781 gagttgattg atattgcaga tttggtaaag gattgcgact ttaatgtatt ttcctctgct
     6841 gcgaatgata attcaggaag agtggttgct ttaaaattac ctaatggatg tgatcttagt
     6901 cgaaaggatc tggataacta tggacaattc gtaactatat atggggctaa aggacttgca
     6961 tatattaaag tcaacgattt aagtgctggt atggcgggtt tgcagtcacc aattctaaag
     7021 tttttatctg aaacggctgt gcaatctatt cttaatcggg ttgaggcgca gactggcgac
     7081 gtcatttttt tcggtgctga caaagctcat gtagtaaacg aatctatggg agccttaagg
     7141 aataaactgg gtcatgatag aaatctgatt aattctggat ggcaattact gtgggttgta
     7201 gattggccta tgtttgaatt ggatcctcag agcaataagt tacagcctat gcaccatccc
     7261 tttacatccc ctcaggaact atctgccgag gcacttcgct ccaagccaac gcaaacatta
     7321 gccaaggcat atgatatagt tattaatggt tatgaaattg gcggtggatc tattcgtatc
     7381 catcaacctg agctacaaaa aactgtattt gatttgatcg gaattggtga gcaagaggca
     7441 caagagaaat ttggattttt gctggatgcc ttacaatatg gtgctccacc tcatggagga
     7501 attgctttgg gaattgatag attagctatg ctgctgacag actcaacttc cataagggat
     7561 gtgattgcgt ttcccaaaac tcaaacagca tcttgtcctt taacaagtgc gccatcgcct
     7621 gcgggcaatg cacaattaac tgaactgggt ataagacttg cgccaactat tacgacaaag
     7681 taatttaaat ttaagcacca caagtttaaa atatttgtgg tgctttttcc tgcctctgtt
     7741 tttattattt tttagttttt cactcttttc agcatcggtt aaagaaaaca atgccacgct
     7801 agtagaatat ttggcacatg aaaaaactca tttgaccatt gctatcaatg aaattaaaca
     7861 aaagagcgct cccaaaaatg agcaagagta caatgagaag ttaagacaaa ttagaactat
     7921 tctttcaatg aataaagcaa aaattgagag tttgaatggt tttagtgaaa atcaaaagaa
     7981 agaagaagtc aatttgaatg agcaactaaa gcgtttgcaa caaatgccgc ttgccaatgc
     8041 tgaagtaact attcaggaaa aagtaggtaa aatcgagacc ttgcttactg ttaataaaaa
     8101 aaccactgag ttaattgcag aaactttgga tttggccaaa caatatcaat tacttttaaa
     8161 tgatgttttg aatgaattaa aactttggaa agccaacttc gatttgaaag aaaagcttaa
     8221 gtcaattaaa gccattagag ctaaattgaa taaagaactg aatatccttt atgagaatgc
     8281 agtatccagc caagataaaa agcaaccgga actaacgatt agcgaacaga ttcaatatga
     8341 agctaatttg tacatcaata atcaaagaat tgcagttatt catcaaaagt tgaatgcatt
     8401 ggagttacag aaaaagataa ttaaaacgaa tatcctgtta ttaaataact tggatactaa
     8461 agccttgcaa tcagcgattg ataattacag agaggttatt aatcaatatt cggctataga
     8521 gagttctttg caaacaattc tgaattctct tacaaatcag gccaatcttt ttgttgagcc
     8581 agggttacaa aaatccgtta gcttattgca gaatgacgtt aaaacagaat taaaggaaac
     8641 ctctaatcaa aaacttatct tatccaaaaa tttagaggat tatcaaaatc aattaaagaa
     8701 acagatttca acaagacaaa gcttaagcga atacaatata gacagttggg cagtcattct
     8761 caagaagcta attgatatcc ccaatttgtt ttataaatac atcaaaacac taaccataaa
     8821 ggtttacgat agttatacct ggctagatac ttttcccgct atttttttat ggacagttct
     8881 tatttttatc ctgagcctgt tttatatttt aaatcatgtt ttgaagactt tagcaagaga
     8941 taaagaacgc tcaagattta ctggttatct ttatggtgga atactcgttt tactgcaaag
     9001 aaacatacct catctttgct tcttcgctat gttatccttc attttatact tcaccagtat
     9061 ttcttatgct aactctcaat tattaattaa actcattatc gtgtggctta cctttagagt
     9121 tttaatttta atagcccgtt tgattttgtt agaaagtata tcggattcat caggaaagga
     9181 cgttaaatta tattatcgac taaaatggtt gttgcttttt ggtggatgga caaccgcgtt
     9241 gatgacattt agtcacttgt tacctttatc aattctactg caagatatat ttaatcgatt
     9301 attcatgctt tttatgttaa cggtatcctt ggtcgcatgg aaaagtaaag atgtaattcc
     9361 ttatctcatt agaccattac tgaaatcaaa gaaaagatat tttaaaaatg caatttcttt
     9421 attggtcatt ttagttccta taaccttatt tacaaccgca attattggtt taattggctt
     9481 tgtgaatctg gcatggacta tgagtcgtta tcaagcttat attctattgg ttttagtagg
     9541 ttatattctc gtaagaggtt tgatatttga tgcactagag cttttctctg agtggatgat
     9601 ctcttcattg aaaaatggtt ggttatggat agaggttttc ctcaaaccaa tcgataaaat
     9661 cttgcgtatt gtcttgtttt taacaagtgt gttaatttta tttcagttgt ttggttggta
     9721 ctctgattca ctggttgtga ctagcctcga aaaagtagtt caatacagta ttcttaatat
     9781 tacaggcata catattacag tcgccagtat cattgaattt cttattctgc ttgctgtttt
     9841 agtatgggca gcaaagtgga caagagagtt ttgctttcga tggctatata agaataccaa
     9901 agatgtgggt atacgccata gtttatctgt atttacacag tatgcgatca tattactggg
     9961 tacattcata actcttcatg ttctgggact ggattttagc ggtatgtcga tgatacttgg
    10021 agggcttgct gtcggtatgg ggttcggttt gagagatttt gcttcaaata ttgttggagg
    10081 cattatgctt ttaattgaac gacccgtaag ggaaggcgat ttgatcacca taggtgaata
    10141 cgaagggcaa gtggctcaca taggcattcg ctcgatgcgc gtatcttcat gggataatac
    10201 tcaggttttg attccgaatg ctgaaacgtt caataaacct tttacaaact ggactcatca
    10261 agatggtatt attcgtacgg tcataccaat aaaggtaagc agagccgatg atcctgtgat
    10321 gattcaacag ttaatacttg atgtattggc aattattccg gaaattgttc cagatcctcc
    10381 cgcacaagtt tttttaaaga aaatcgatac agctttaata gaatttgaag cacggtattt
    10441 tattaacatc cagaatcata accgggttga agtaaggtca aaagtcttgt ttgccataac
    10501 tgcacagttt aaagcggcag gaatacagcc gccaattgag cctattactg ttgaactaaa
    10561 agagagctcc agtgatttta gccttaaaaa gcaaaccacc gaagaatgaa aaagaacttt
    10621 tggagcaatg ccagcgaatt gaagggttga gtttcgctca gttagctctt gaattgggtc
    10681 tatttacccc tttaaaccct gttcatcgaa aaggatgggt cggacaagct ctcgagatag
    10741 ctttaggtac tgatgcaaaa aatgagtcgc tacctgattt taagcagttg ggcattgaat
    10801 taaaaacgat acctattaat aaatcaggga aaccggccga atccacattt attgtttcta
    10861 ttccactact gaccatccat caacaaagtt gggagtcttc tcaatgttac gctaaattaa
    10921 aaagggtatt atggattcct atagagggag atacagatat tccttttcct cataggagaa
    10981 ttggacgtgg atttttatgg tctccgaata aagatcagaa atctgttctt gcagaagatt
    11041 ggaattattt aacaaaccaa attgttctgg gacaattaga aacattaaac tcctcttcgg
    11101 gagagtatct tcaagtgagg cctaaggctg ccaatggaaa atctttatgt tatggttatg
    11161 acccagaagg taatcgtata agaacgttac cacgaggatt ttatttacgc tcctgtttta
    11221 catccgtaat tttaagtcaa tcctaatttt aaggataaag taagcaccat ctgtttaccg
    11281 atattatgat aaattttaca gatattaggg aaagctcatt atagcttttg tcgattagta
    11341 tgcatggatg atgtttgttc agatgctgtt gctggagtca ctattgattc tcttaatgta
    11401 tatggttttc tgttaaataa taaccatttg ggatcatggt agggtaactg ctcttctggt
    11461 ttggccttac tagaatgcat tttatccttg cgaatattat gagagtgata aggttcttct
    11521 ggtgttctgt tctccggatg ttctattgta ctgtcatgta tggtagtgga agaataaaga
    11581 gagcttatac ccagcattag cgtggcacat gctaacaatg caatcattat tcctaacgat
    11641 gcagggttaa aaataacggc taaaccagct actgagcttg cagaaaatac aaagagactt
    11701 acacctatag ctaaaatagc ggcggtggca atacaaacaa aaatggtatt gcgagtgaag
    11761 ttggatagtg atacttgtgc ttcacttgtt ttgggtaacc agagcgcaaa gacttgatca
    11821 ttaagcttaa ttgataattt atttaattga aagagctgta tgtttttgtt cgcagggaat
    11881 tgtaaatctt taaagacgtg gtttttattt gatttcatcg agtttgcaat aattgctgtt
    11941 gttggtaatg gacccaaatg agcatgggct cgaatttctt tttgccatgc tttatcaatt
    12001 tctattgcat tctcttgata ttctttgtgt aattgcatta ataaactgct agtgatataa
    12061 aaatgaacgc tatcaagaat aaatcgatct aaaacgctaa tttggttatc acgtggatca
    12121 aaattatcaa aattaccgaa aaaatatcga attctatttt gattatgtgt ccctatgaaa
    12181 tcgtaaattt ttttatgatt attaattaac aatttaaaaa atatttcgtc aggaagctct
    12241 tttatatcat accttgcttt aacacattct tttaatttgc tgatgtcgga tatctgctct
    12301 agaaacattt ttttgtattg attgtaaagc aattccttgt ttgcaaaaat agttttcatc
    12361 ttgagaaacc tttgtggagt aaatcaatgg cattatttgc tgttaatcat cctcatattg
    12421 gccttaatga tgattatgcc tcctttcttt aattctaatg ccaaccttta ataaggctta
    12481 aggtcggcaa gaaaaatatc agctctttac ctaatacgac cattaggtaa tatttaagca
    12541 tgagggatct attaccatta ttttgaatta gatcccgcca acattcctgg tatggttgtt
    12601 tgcaacaaat ttttaaatga gttgacaggt tgagttagct tggctttcaa tagttctcta
    12661 aaatcagagt tcgtagcaaa atacttgact gcctgctcaa aaccagcaaa aacacctaag
    12721 tcaaggcctc ttgtttctac ctgcactttt aggtctttaa ttcgttgcac ttgatcctga
    12781 ctcggtttac tggctgttga gaacagtgaa aatggcgctg atactataga atagcctatg
    12841 gacaaagtgt tggacaggat tccattttga gtttctgcag tttttacttc tttaggccag
    12901 gcaatcataa aatcagtaga gcataaaggg gcgattaatt taatcatgcg ttggatatca
    12961 ggatacaaaa ggttattggt attatcgata tcggtactat agttttcttt gttatttttt
    13021 agacaccaat tatagagtac tttcatctct ttcaattgtt tataaaaatc ggtcatgcta
    13081 aatgattcaa gaatgtaacc agaaacgcct aaatagagac aggctgcaag aaggtgattc
    13141 gcaaattcag gaataggcaa aaaccttcca actatccacg agccccccca agctgttaat
    13201 gcagtactca ttgtttgata gaggtacatt tgattgacac ttaactcaaa ttgtttgatg
    13261 tacttttcca atgcctgagt gtgttcctta ttatccaggt tgaagggaga tgacgtcaga
    13321 actcttaggg cttctttcct tttggctctt tcaactccga cttcagccat tccaactgcg
    13381 tttaccttac ccacaattct ctccttgaaa tcaaaatttc attttatact ataaatttaa
    13441 aaaataatga attagatttt agctaagagt tcaattaatt taacttaata aaaaagacga
    13501 ggtataataa aaccgattaa tcaaattgaa gtcaatatca tgcctcgtac aatgtttttt
    13561 tccgttgatg cagctaatct ccaaaattta caaattacaa atcgaaaagc ttttgcagcg
    13621 ttggagagtt atctgaatga taatgattca ccaggtgcct ggaataataa tcaaggatac
    13681 aaatatactc ataaggatgt tacttattgc ttctcattta atcaatcctt agtaagacgc
    13741 ccaagaaaat cggatcaaaa aaaacataca tttgaaattt ttgaccctaa taagaaccct
    13801 ttaggcaagg gagcgtacgg tattgtctat cccattttag gtgcaattca gtttgaattt
    13861 ggaaccgcaa ttaaaagacc accaaagaat aaattagtta aaatacaaaa ccatagtgaa
    13921 cgcgatcaaa gtcattctgt actacgtgaa tatcaagggc ttcttcaatc agggcatata
    13981 gcggttaaac cgccaatatt tgtagaaaac aaagagagta aattcagtta cttgattatg
    14041 gaaaaagcgg agggtatcgt tttagagaag tttctaaatc ccgttaagcg gcttgatttg
    14101 tctgaagagg tacccgagct taatcttatt aatcggattg agattacttt tgccatttta
    14161 aaagcaatta aagaacaggt gacggataag catttgattc atcgagatat aaaaccagga
    14221 aatattataa tcgattttag caagtcacct ccgatagcta aagtaatcga ttttggtttt
    14281 gtgcttaggg cgtcagaaca ggattaccgt cgttgtggaa cccgagcata cagagcgccc
    14341 gaatctttca atacccaggc attgtacaca gcaaaagctg atgtttggtc aacaggcagg
    14401 atattaagtt atttgtgggg agataaatat actaattact atatctctcg tgataagggt
    14461 ttggactacg tgattgagaa gtcaagaaac gaactactct tctctgaccc agaattggaa
    14521 ctctatttaa ccaaggaaga taaaagtaaa atcagatcgt gcctaagcgc aatgcttata
    14581 gttgaccctg aggaaagagg atctatagat aaggctatca aacagttttc ccaaattgat
    14641 tttgaaaaat ataaaagatt aaagcgctct acaaattttg aaattgattt aacagataat
    14701 gatattaaat taagaaaaca gctcaattca attcacctgc atttgattac cctacagggc
    14761 aaagaaaaag atttgcgtga tagagcatgt tttgatgcag cgaatgcaat gagccaattg
    14821 gttagtaagc tctctaccta caccaattat cttgaaaaaa atccagatcc atttttaata
    14881 aaacgttata aagagtgctg catcgcagaa atagatttag ctaatttggc tttgcaaaat
    14941 caccgagatg ctttatggtt ggtggctgag ttatcaactg ctatcctttt gcttggtata
    15001 gggtatttat ttgcttttgg gataaattat tactacacag gtcgcctggg attattttct
    15061 caaaccaggt ctgagaaatt ggtggaagaa atgaaaagtt cggtgttagg tattgcggtt
    15121 ggaaattaaa aatctgtatt tcccggatta taacagagct gtctaatccg ggttattcat
    15181 ggttagattc ttaacacttg agattgagcc atattcaggt tttctaattt ttgcaaggaa
    15241 tccgtttcaa aatgaaagaa aaaatgacgg ccctctgttt tataatagtt aatggaagct
    15301 gcgagcaaat agagaacacc aagccctata atggccgctc ctacattaac taacaaaggc
    15361 ttccatcctc tatgctgttc cagtacaggt cgagccctct caatttctct ttggcatgtt
    15421 tgtttgaaat tttcgtatac ctcgggtgtt ttatgataga aatacagttg ggaggctatt
    15481 aataattcct gatgcagtct gttagctgct tttgacgcag actcataatg ataaggatca
    15541 acttctgctt ttttgcttaa ttcctcagcc ttttttctga tattttccaa ttcggtatta
    15601 aattgggtag tgaatgaagt tttggtgagc tccagctcat ttaaaaatgt ttctttttca
    15661 tcttgagaga ttaattcaga attaacaaaa gaaagtaatg tatttttgag tttggcatcg
    15721 gttatttgtt gagaatactt aatattaatt tgaatctgat ccatgtcatc ataggtgatg
    15781 ctagggatac ctcctataaa ataaagaatg tacagattat tgaaaagggc agtaattctt
    15841 tgcagtgcct ctctattttt aggacaaatg gttagaacat tatgagctag attgatatca
    15901 attttttcac aagaatgttt aaacgatgac atactaaacc tcatataatt cagagatttc
    15961 cctatgcaac aattcatttc ccttgaaggc aggtcaagta tatgtagaca agcttaagat
    16021 tttattaaga agcgccttaa atttaacaat ttttcaaaaa aactttacga ttttttgaaa
    16081 tgcaaaaaat atcgttaaca cttgcagatt tcttttataa aaacctacac acttcatcca
    16141 cttgatggtt accttgccga aagtccagac agcaggtaaa atcaatggga aaacaggtaa
    16201 aatccgtggg ttattagcag aatcccaata attaatacca ctatggaaat cacataataa
    16261 cctgtccctg tataggcttt ttgactgcat ttcaccataa actcgggaaa tccaagccat
    16321 aatattgatt taatcaaaat aagccaggct accagtgtaa ctaatatctc tgattcagga
    16381 acccaaatgt tatggaccag tacaaggcaa atacctataa ttaaccccaa gcttccagcc
    16441 gttacaatag tggcactgcc ttctttgaga tgagtcaaca attcacgata atatgaagct
    16501 cgagaaagca ttataattcc cacaataaga agatagaggc cgattgcttg tgccaaaaat
    16561 aaagagtgaa ttgattcttg catagttggc tccttgtgat tcctctccta aattaattat
    16621 agtacatgcg ggattgggtt tgttaaccag gcaggtactt gctagttact actcatctaa
    16681 aacaacttgt gtattatatt tggttctatt aagacaaaag ctgcttctga attttctaat
    16741 attgggctca tttgcgaata ctcgcctggc aaattcattg taatgattca tatctttgac
    16801 atgaataata agcaggtagt ctgtgtctcc cgagacaaaa taacattgca taacctcagg
    16861 ctcttcctgc atttttcttt caaaatgagc tagcagatct tctctttgtt tttcaagagt
    16921 aatattgacg aacacaatta atggtctgcc tactttgaat ggatcaacca aagcaacatc
    16981 gttaacgata attttttcct ctctcaggcg tttgactctt cgaaagcagg gtggggcaga
    17041 gattccaact atatctgcca atgcctggtt agtaatttga ttatttttct gaagaatatt
    17101 tagaattttt ctatcaattt tatcaagtat ttcaactgga tatcctgtat ttttttcat
//
LOCUS       DATNSN010000060        17098 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.060, whole genome shotgun sequence.
ACCESSION   DATNSN010000060 DATNSN010000000
VERSION     DATNSN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17098
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.060"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(35..1021)
                     /locus_tag="VAH76_001602"
     CDS             complement(35..1021)
                     /locus_tag="VAH76_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEO1386230.1"
                     /translation="MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPA
                     YCVSEMCSAIDILHKHSSQSRYIYRAPTEKLLAYQISGNEPYLLAEAAAKLQSLGADL
                     IDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
                     AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKA
                     LEISACDGFMISRAGTGKPWLYRDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKA
                     VLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRVSNILVAAS"
     gene            1103..1546
                     /locus_tag="VAH76_001603"
     CDS             1103..1546
                     /locus_tag="VAH76_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386231.1"
                     /translation="MDRRYLSPLELLGIATQHAYTADYMLQQIANGMYRGGETIAVFS
                     PITSLMYVAFQLTFKAYCLHDHRPIKEYKNLMELVELNSHLGLSSNDIFLLKTLSRQQ
                     VFNKGVDYDLWENQQQLHVFCEEIISLYERVQSMMPLELQSDYQE"
     gene            1723..2718
                     /locus_tag="VAH76_001604"
     CDS             1723..2718
                     /locus_tag="VAH76_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEO1386232.1"
                     /translation="MEQLDDKVADNVQQKILQLSRHLNSRILGQKDLISRLLIALLAD
                     GHLLVEGAPGLAKTRAVKELSDVVEGDFHRIQFTPDLLPGDLTGTDVYHPQNGSFVFQ
                     PGPIFHHLILADEINRAPAKVQSALLEAMAERQVTIGGKTYPLPELFLVMATQNPIEQ
                     EGTYPLPEAQLDRFLMYVKISYPDAKVEHDILTLARKEALGNATENQIKVTKLSQKTL
                     FEARKQILKVHTSEALENYLVQLVVATRNPGVYSDELGRWLRFGASPRATIALDRCSK
                     AHAWLAGRDYVTPDDIHVIAHDVLRHRILLSFEAEAEGISSDDFIDSLLRLVAIP"
     gene            2727..3638
                     /locus_tag="VAH76_001605"
     CDS             2727..3638
                     /locus_tag="VAH76_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEO1386233.1"
                     /translation="MTDGVVAELSELIDLRRYVHSVHYKPQGRALRSGNHISKFRGRG
                     MDFAEVRNYQAGDEIRHMEWRVTARTGRPHVKIYQEERERPVVLLVDFNPSMIFGTKI
                     AFKSVVAARLASLLAWTVIKQGDRVGGFFFSATEHSEFIPRGRDSGVLPLLASLSRYT
                     TQTEAQQEEKPRMLSDALMRLRRVIRPGSILVLISDFYSMDVECEKHLNRLRGHNDIL
                     AYHVCDRIELAPPKPQQYAISDGKQEIILDTGLETVSTAYQLYCQQRIEQLNDQFKRL
                     QIQYVQVTADTNLTQLVRLTFPRRSRG"
     gene            3631..4116
                     /locus_tag="VAH76_001606"
     CDS             3631..4116
                     /locus_tag="VAH76_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HEO1386234.1"
                     /translation="MAKAEPLAQLRDIHLPEPVGWWPLAPGWYGLMVLIVVLVAGIAY
                     FIYKRHVNALPKKQALGLLKIYKEQYEKDKNTQLASAHISELLKRVALVYYPRAEVAS
                     MHGEAWVEFLNQTGKGIDFTPVKSMLLDSPYKTSEALNLNPLFTRAEKWIKQRGAPCS
                     N"
     gene            4104..5138
                     /locus_tag="VAH76_001607"
     CDS             4104..5138
                     /locus_tag="VAH76_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO1386235.1"
                     /translation="MFELATPWALLLIPVPLFLWYLLPRAKVKLPAALKVPFFEAMAG
                     IVEQENRLISAKTLLLIPVLVWVLLVIALSGPRWVGEPKPVAREGYNIMMVLDLSGSM
                     EITDMLLHGRPVSRLLVVKRAAEQFVEDRVGDRIGLILFGTRAYLQTPLTYDRHSVLM
                     RIDDATAGLAGKTTSIGDAVGLAVKRLQDVPSKGRVIILLTDGANNSGVLAPLKAAEL
                     AKQDGIKIYTIGLGSEADPRALTGDFFAPTLSAELDEKTLEKMAKMTGGRYFRATDPE
                     SLQSIYQTINQLETVKQEQATVRPQKEYYPWFVAFALLLCFYWLISKADLFVYLRSFL
                     KVERSLSHDS"
     gene            5128..6060
                     /locus_tag="VAH76_001608"
     CDS             5128..6060
                     /locus_tag="VAH76_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO1386236.1"
                     /translation="MIAEFHFLRPWCLLMIVPLLALVVIYWRQKPKLHAWEAVCDPHL
                     LEHLLHSKGQSKRALSLSFLFFSILFMILSVSGPTWYKLPQSTYKPIQPRVLLLDMSD
                     KMMLGDLSPNRLSRAKFKLHDLFTRKDIGQFGLIVFTGEPFVVSPLTDDGQTISSLLS
                     TLTPDIMPVAGYNLDSALDEASQLIKQAGYDHGEILVLTAETPSSSAIEKAKKLAESG
                     IFSSIMPIRADENLNPLFQRFANAGDGYLLVYSPDSSDLEKWLNASQGKEKFALEKND
                     DIPLWRDEGRWFLIPALLLLLPVFQRGWMQRVTI"
     gene            6057..6983
                     /locus_tag="VAH76_001609"
     CDS             6057..6983
                     /locus_tag="VAH76_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO1386237.1"
                     /translation="MMWRVLTIVAVALISNQLKALSWSDLWFTPNQQAQHLMEKGQFA
                     KAKDLFERNDWAASAAYRAGDYEQAAKLFNELKTEQGYYNQGNALAHLGKYEEAIRAY
                     DKALAFNPNNQDALYNRKLISELLKKDKEKKKSQNQQDKDQQNKDQQNQDQQNQDKQN
                     KDQQNQDQQNQDKQNKDQQNQGQQNQDKQNKDQQNQDQQNQDKQNKNQQNQDQQNQDK
                     QNKNQQNQDQQNQDKQNKDQQNRDQQNKAQQNKNQNKKNDKQEGEIQSESNQEKQLAK
                     EQWLRLIPDDPGGLMREKFLRDHLRRERGWYQ"
     gene            6980..8629
                     /locus_tag="VAH76_001610"
     CDS             6980..8629
                     /locus_tag="VAH76_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEO1386238.1"
                     /translation="MKKLMLWVLLSLFSSLSFAEIKVELEPSQVTMGETFKLSITQED
                     QQGGGVPDLTELQKDFVILGTERHVNYSIINGQAQSQSQWIVLLRALKPGIFTIPPIK
                     VGLEQSLPITINVQGSANTQALPDEAIQKQDVFLTAEVNEKNPYVNQQVIYTVKLYNS
                     KRLLDADYQGPKVDDALLIPLGDAKRYHAVHNNINYVVEEQTYAIYPQKSGTLKIISP
                     IFNALVYDYNPQRTKATDKAISLTIKPIPKKYQGKLWLPAKQVKLSEQYENSNQTLSQ
                     GSTLTRTVTLEGIGIPAQLLPTLTFQETDFFSVYPEKGAERNQVKQGELIGSVEIKVT
                     YLFNKAGKTTIPELRLPWFNTETGKEEIAVLPPRSIDIIPSTAVKANTANTQVTGKEM
                     SASSQSAPDSASVANESGGWAVKLAIFFGLAWLITLSLWIWQKRPRESSSKKEYKKAL
                     DALNKACSQCNPKKARDAVLKWASLQWPDAPMLNLTDLAQLVRDAHLRKQLHLLSQVL
                     YRSQEKTLWRGDELLRAVQAVKRNESVKKRKNSNLPPINPF"
     gene            8866..10248
                     /locus_tag="VAH76_001611"
     CDS             8866..10248
                     /locus_tag="VAH76_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO1386239.1"
                     /translation="MHVNEYIHYDVQELAKLIKNHQVGAEEVLRCAQERLYQVNPSLN
                     AVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFANNLAEINS
                     DLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIM
                     AGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPWVDEAWSGMATSFILTRSM
                     SDAIALFKNVINPMRLNRLSRKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNS
                     GHRLNEVHLPLDLQVISSCAHTLIAASTYAKVKTQEQISGRKPMIHELEPITWEFYYR
                     GKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRIDEEFNKYL
                     QKKKEFSPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQPVL
                     PKNITPASIP"
     gene            10529..11986
                     /locus_tag="VAH76_001612"
     CDS             10529..11986
                     /locus_tag="VAH76_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1386240.1"
                     /translation="MKTLPSKVIFIGAGPSGLFAARQLKEIAKLQNKTVECIFLEREP
                     VVGGKCHTYSDAVHPELKTEWGAALVAPNYGVVLDALAEHDIKFEKVMPTASETIEIK
                     QLFEKASSLEKISMEMKLLRELWAFNSDYDVYKKAKQNKKALPDKLLNPFSEYCKLNN
                     MSYLPVFLKPFVPGFGYGDLNFCPAYCVLEYMGKVTMPNILLADQLIGRPSLLAIHGG
                     FQLLMERIAEQFDVRTSASVSRIKREKNKVTVTYVHNGIEQTETADTLVLATSPKNWK
                     SLGLDLTEVENNCIESLDYYRYPVAVYKIKGLPPQQYFFTKALEERGFGHLALITTRD
                     NRSNPEDGRLCTVYVNLSPNNNEFKFDHEALRKELESIPDVTEVTVVKDKIWEDYMST
                     LPWDLRLQLDKEQQRTNTMYLGSYALGSFEDVACVANKATDAMTEWYAPTMRYEEDFS
                     WKNMNRAWQFFTSPTKSPLASLGEPRMSDEHSNRP"
     gene            12225..12440
                     /locus_tag="VAH76_001613"
     CDS             12225..12440
                     /locus_tag="VAH76_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO1386241.1"
                     /translation="MSVKETGHVKWFNDDKGYGFISRDNGQDDVFVHFRSIVSSAGRK
                     TLLEGQRVEFLVTKGAKGLQAEEVTAI"
     gene            complement(12541..13845)
                     /locus_tag="VAH76_001614"
     CDS             complement(12541..13845)
                     /locus_tag="VAH76_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386242.1"
                     /translation="MTQQDPVVIKSKGDQALEKGDLPLALTLYDEALAINPQFSGAYY
                     QKGTVLLRMGKAIEAAAMYWQAYLFSEFKTDIGLMTAKTLAHANYSLSACKLFESFKI
                     EEMDGESLAYYLYALRQQGRAQEAYALFPYVNQFNIPKTSWARAIILLDLNKVEEARF
                     LLEPLEKTDKDGHITILLHAVYLALNDKKAVKSLLDRAIQRIPNNDYFCCQRIAIDIL
                     NGLNKTPIETYRAFKRFDLIDAADYLHKHADKHLRHSGTTYQTFDLLAPQVLSEGLIL
                     EFGVRFGYSISHIAKLFPKRKIFGFDSFQGLPEDWYHEKAGSYSTYGKIPSVASNVTL
                     IAGWFNETLPDFKKNNREPIAFMNIDCDLYSSTKLVFNELNSQIAPGTIIVFDEYIGN
                     INWRQDEFKAFQEWVKENKVEYRYLTASFYTKQVSVQILKRK"
     gene            14008..14988
                     /locus_tag="VAH76_001615"
     CDS             14008..14988
                     /locus_tag="VAH76_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEO1386243.1"
                     /translation="MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNP
                     DNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKAICDLS
                     PGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
                     YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTD
                     GYFSLAMRLTTEVNNTEFGQYLTAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNG
                     ILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDFSNEKWL"
     gene            15127..16089
                     /locus_tag="VAH76_001616"
     CDS             15127..16089
                     /locus_tag="VAH76_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEO1386244.1"
                     /translation="MLKRCYLLILLMFVLASCAHHKPQTPPAEVKKQGTSSTRQFRQV
                     SSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTVVKQNTLYVSLGQGYPDYG
                     VVTVDIKTKFLNRFRYEGAGVVTGNNIRTSYLDLYLANEGTTRLAGNIGLQKLEAVGS
                     GVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKK
                     AAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDA
                     SDIYYYNLSKTRADFMAFNGSVLDMREWSQSDLKDFDRYNKQFP"
     gene            complement(16181..>17098)
                     /locus_tag="VAH76_001617"
     CDS             complement(16181..>17098)
                     /locus_tag="VAH76_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn1 family transposase"
                     /protein_id="HEO1386245.1"
                     /translation="IVVLIDWTGGCSEGYHVLEASIAAHGRSIPIYHEVHSESEQENA
                     EIHRQFLLRLKEVIPSSLSVTIITDAGFHREWFQQVLELGWDVIGRIYSLYCYQIEGE
                     TNWHKVKDILFEGIGKASALGKVKLGKTKKAVEGYLYTYKEKLSGKVRKKKNKYPSHD
                     KAHSNYYKNGWVLFSSLNKHARFLVSYYKKRMQIEQNFKDIKNEQLGMGLRRNQSSGK
                     TRVNMLFFLAVLLIMIAWWFGLMIESLNKHRSYQANTIKNKRVRSFIHLARMAYRHEP
                     ELLNWDLFQYIMSDLKQQYHSFIECGALS"
BASE COUNT         5216 a         3136 c         3639 g         5107 t
ORIGIN      
        1 attatagaaa taatatacaa atcctgacgt tatatcatga ggcggcaact aatatgttgg
       61 aaactcggtg agttgcctca gctaaaaaat ccttgatttc cattaatgaa cctaattgat
      121 agaactgttg cagttgttgt tcgttcagtt gctttttgaa gtaatagcga accagagatt
      181 tactttgcag tactgcttta tgctcgtttt caaggtatga caatccttcg aggtggagca
      241 taaatagttt taatttctca tcaaaagcga catcatttgt ttccagattc aataagtctc
      301 gatatagcca gggtttgcct gtaccggctc gagaaatcat aaaaccatcg cacgcgctga
      361 tttccagggc ctttcttaaa ctgaatatat tgttaatatc accatttaca atgactggta
      421 tgttgacagc attcttgata tttgcaattt gctgccaatt gcatgggata tcataatctt
      481 ctgtccatcg cctgccatgt actatcaggg catgagctcc ggcgtcctct atttgtttgg
      541 ctaaggttaa atccttttca ttattttgaa tgcgtatttt aatagtcagc ggaatgctaa
      601 ttgctgcgcg taccctagag ataatggcaa tcaatttttg tggatcttcc aataaagcgc
      661 taccagcccc ttttttacga attttgcttt tagggcagcc acaattaata tcaattagat
      721 ctgcacccag tgattgtagt ttagctgctg cttctgcaag aagatatggc tcatttccgg
      781 agatttggta agccaggagt ttttctgtgg gggctctata aatataccgg gattgagagg
      841 agtgtttatg aagaatgtcg atggcagaac acatttcaga cacgcaataa gcaggcggtg
      901 tataaatatt gaatagatct ctgaaagggg cgcaactgta acctgccaaa ggtccttgaa
      961 tcagtcgatg agggagtttt aaagagccga tttgcagagg actgttaata aaagtgtgca
     1021 tcaatggaat taaaggatat aatcgctttt tattttaaca tgattcacca tgaagaacca
     1081 gaaaaaaggg agaggcttcc atatggacag gaggtatttg tcacctttgg agctgcttgg
     1141 cattgctacg caacacgcct atacagcaga ttacatgctg cagcaaattg ccaatggaat
     1201 gtatcgaggt ggggaaacca tcgcagtttt ttctccaatc acttcactca tgtacgttgc
     1261 ttttcagcta accttcaaag cttattgttt gcatgatcac agaccaataa aggaatataa
     1321 aaatctaatg gaactggttg agttgaatag tcatcttggt ttatccagca atgatatttt
     1381 tcttttaaaa actttatcaa gacagcaagt gtttaacaaa ggagtagatt acgacctttg
     1441 ggaaaatcag caacaattgc atgttttttg tgaggaaatt atttcattgt atgagagagt
     1501 gcagtcgatg atgcctctgg agttgcaaag tgactatcag gagtgagcgg ctgaattagc
     1561 gccctgttaa gtgtctttgc ttaagttttc ttatgttgtg catggcccag gatgcattaa
     1621 tcagatatca aaatgattta tttttatcca aaatagactc attctacttg gcatcccaat
     1681 ggattcaagt taggatatta tttttttaaa agggtaattg tcatggagca actagacgat
     1741 aaagtcgcag ataatgtgca acaaaaaata ctacagttaa gtcgtcatct taattcgcgt
     1801 attttgggtc aaaaggattt gatctctcgt ctgttgatcg ctttgcttgc ggatggccac
     1861 ttgttggttg aaggagctcc gggtttagca aaaaccagag ccgttaaaga attgtcagac
     1921 gtagttgaag gtgattttca tcggattcaa ttcacccccg atctgctacc tggggactta
     1981 actggtacag atgtctacca tccgcaaaat ggttcttttg tctttcagcc cggccctata
     2041 tttcatcatc ttatcctggc cgatgagatc aatcgcgcac ctgccaaagt acagtcagcc
     2101 ctgcttgaag cgatggcaga aaggcaagtc actattggag gaaaaactta tcctctgccg
     2161 gaattgtttc tggtcatggc aactcaaaac cctatagaac aagaaggaac ttatccttta
     2221 ccagaagccc aattggacag atttttaatg tatgtgaaaa ttagttaccc tgatgcaaaa
     2281 gtagaacatg acattttaac tttggcacgt aaagaagcac tgggtaatgc aacagaaaat
     2341 caaattaaag tgacgaaatt gtcccaaaaa actttatttg aagctcgaaa acaaatattg
     2401 aaggtacata ccagtgaagc acttgaaaat tatctggttc agttggtagt tgctacccgt
     2461 aatcctggtg tatacagtga cgaattggga cgctggttgc gttttggagc aagcccacgc
     2521 gctactatcg cactggaccg ttgttccaag gctcatgcct ggcttgcagg tagagattat
     2581 gtgactcccg atgatataca cgttatcgct catgatgtac tgcgccacag gattttgtta
     2641 agttttgaag cagaagcaga aggcattagc agcgatgact ttatagattc tttattacgt
     2701 ttagtcgcca tcccttagag gtttttatga ctgacggagt tgtagctgaa ttaagcgaat
     2761 taatcgattt aagacggtat gttcattccg ttcattataa gccgcaagga agagcgttgc
     2821 gttctggaaa ccatatttcc aaatttcgtg gacggggaat ggattttgcg gaagtaagga
     2881 attatcaggc tggtgatgaa attcgtcata tggaatggcg cgttaccgcc cgtactggaa
     2941 ggcctcatgt caaaatttat caggaggagc gggaaaggcc agtggttctg cttgtagatt
     3001 ttaacccttc catgattttc ggcaccaaga tagcatttaa gtcagtggta gctgcacgat
     3061 tggcttctct tttagcatgg acagtgataa aacaaggaga tcgtgtagga gggttctttt
     3121 tttcagctac agaacacagt gagtttattc cgcgaggacg agattcgggc gtactccctt
     3181 tattggcttc tttaagtcga tataccactc aaacagaggc tcagcaagaa gaaaagccac
     3241 gtatgttaag tgatgcctta atgaggctgc gtcgggttat tcggcctgga agtatactgg
     3301 ttttgataag cgatttttat tctatggatg ttgagtgtga gaagcattta aatcgtttac
     3361 gaggccataa tgacatactg gcctatcatg tttgtgacag aattgaactg gctcctccaa
     3421 agccgcaaca atatgccatt agcgatggta aacaggaaat catattagac actggtctgg
     3481 aaactgttag cacagcttat cagctttatt gtcagcaaag aattgagcag ttaaatgatc
     3541 aatttaagcg gttgcaaatt cagtatgtcc aggtgactgc tgataccaat ctcactcaat
     3601 tggtgcgctt gacctttccc cggaggtctc gtggctaaag cagaaccatt agctcaatta
     3661 cgagacattc atttgcctga gcctgttggc tggtggcctc ttgcgccagg gtggtatggc
     3721 ttaatggttc ttatcgttgt tttagtggca gggattgcct atttcatcta taaaaggcat
     3781 gttaatgcat tacccaagaa acaagccttg ggtttattaa aaatctacaa agaacagtat
     3841 gaaaaagata aaaacactca attggccagt gcccatattt ctgaattgct aaagcgagtt
     3901 gccttggttt attatccaag agcagaagtt gccagtatgc atggtgaagc ttgggttgaa
     3961 tttttaaatc agactggcaa aggaattgat tttactccgg ttaaatcgat gttattggat
     4021 tcaccctata aaacatcaga ggcactgaat ttaaatcctt tatttactcg tgcagaaaaa
     4081 tggatcaagc aaaggggggc gccatgttcg aattagctac cccatgggct ttgttactca
     4141 ttcctgtccc tttgtttctc tggtatttgt taccgcgggc aaaagtaaaa ttacctgctg
     4201 cattgaaggt tccttttttt gaggcaatgg caggtattgt tgaacaagag aaccggttaa
     4261 tctcggctaa aacgctgcta ttaattcctg ttttagtttg ggtattgctg gttatcgccc
     4321 tttctggtcc gcgatgggtt ggtgagccaa agcctgtagc aagggaagga tataatatca
     4381 tgatggtatt ggatctttca ggcagtatgg aaattacgga tatgctactg catggccgac
     4441 cagtcagtcg tttgcttgtc gtgaaaaggg ccgcagagca atttgttgag gacagagttg
     4501 gagatagaat cggtttgatt ctgtttggta ctcgagctta tttgcaaaca ccattaacct
     4561 atgacagaca tagcgtatta atgaggatag atgacgcaac agctggtctt gcaggtaaaa
     4621 ctacttcgat tggtgatgcg gtaggtttag ccgtcaaaag gctacaagat gttccttcca
     4681 aaggaagagt tattattttg ctgacggacg gagcaaacaa ctcgggtgta ttagctccct
     4741 taaaagcggc agagttagcc aagcaagatg gaatcaaaat ctatactatc gggttaggtt
     4801 cggaggccga tccaagagcg ttaaccggtg atttttttgc ccccaccctt tccgccgaac
     4861 tggatgaaaa gacattggaa aaaatggcaa aaatgacagg tggtcgttat ttcagggcga
     4921 cagatcctga gtctttacaa tccatatacc aaaccatcaa tcaattagaa actgtgaagc
     4981 aagaacaggc aactgttcgg cctcaaaagg aatattatcc ctggttcgtt gcctttgctt
     5041 tgttattatg tttttattgg ttgattagca aggcggattt atttgtgtac ttaaggtctt
     5101 ttttaaaagt agagcggagc ttaagtcatg atagctgagt ttcatttttt aaggccatgg
     5161 tgtttgttga tgatagtgcc tttgcttgcg ttggtggtaa tttattggcg gcaaaaacca
     5221 aaattacatg cctgggaggc ggtatgtgat ccccatcttc tggaacattt attacacagt
     5281 aaaggtcaga gtaaacgtgc cttatcttta tcattcctgt tttttagtat tttatttatg
     5341 attttgagtg tgtctggccc aacatggtac aaattgcctc aatctactta taagccaatt
     5401 cagccaaggg ttttgttatt ggatatgtca gataaaatga tgcttgggga tttatcgcca
     5461 aatcgcctta gtcgggccaa attcaaatta catgatttat ttacccgaaa ggatataggg
     5521 cagtttggtt tgattgtttt tacaggtgag ccttttgttg tatccccttt aacagacgat
     5581 ggtcaaacta tctcttcctt attgtcaacg ttgactcctg atatcatgcc cgttgcagga
     5641 tataatttag acagcgcttt ggatgaagca agccaattaa ttaagcaagc gggttatgac
     5701 catggcgaaa ttttagtttt aaccgccgaa acaccatcct ctagcgccat tgaaaaagct
     5761 aaaaagctgg cagaatcagg tattttttct tcaattatgc caatcagagc tgatgaaaat
     5821 ttgaaccctt tgtttcagcg cttcgccaat gcgggagatg gatacttgtt ggtatactcc
     5881 ccagattcaa gtgatttgga aaagtggttg aacgcctctc aaggcaaaga aaaatttgct
     5941 cttgaaaaaa atgacgatat cccattatgg cgtgatgaag gccgctggtt cttgattccc
     6001 gcattgcttt tattattacc cgtatttcag cgaggttgga tgcaaagggt gacgatatga
     6061 tgtggcgggt tttaactatt gtggcagttg cactaatatc caatcagctt aaggcactta
     6121 gttggtctga tttatggttt actcccaatc aacaagctca acatttgatg gaaaagggac
     6181 aatttgcaaa agctaaagat ctttttgagc gcaatgactg ggctgcaagc gcagcataca
     6241 gagcaggaga ttatgaacag gcagccaaat tattcaatga gttaaaaacg gaacaaggct
     6301 actataacca gggaaatgct ttagcacact tagggaaata tgaggaagca atacgagcat
     6361 atgacaaagc cttggcattt aatccaaata atcaggatgc tttatataat cgaaaattaa
     6421 tcagtgaatt gttgaagaaa gataaagaaa agaaaaaaag ccagaatcag caggataagg
     6481 atcaacaaaa caaagatcaa cagaatcagg atcagcaaaa tcaggacaaa caaaacaaag
     6541 atcaacagaa tcaagatcag caaaatcagg acaagcaaaa caaagatcaa cagaaccagg
     6601 gtcagcaaaa tcaggacaag caaaacaaag atcaacagaa ccaggatcag caaaatcagg
     6661 acaagcagaa caaaaaccag cagaaccagg atcagcaaaa tcaggacaag cagaacaaaa
     6721 accagcagaa ccaggatcag caaaatcagg acaagcaaaa caaagatcag cagaaccggg
     6781 atcagcaaaa taaggcacag cagaataaaa atcaaaataa aaaaaatgac aagcaagagg
     6841 gagaaattca atccgaatcg aatcaggaga aacaacttgc taaagaacaa tggttgcgat
     6901 taatacctga tgatccgggt ggattaatga gagaaaaatt tttacgtgat catttacgta
     6961 gagagcgtgg gtggtatcaa tgaagaaatt aatgttatgg gttttattga gtttattttc
     7021 atcattatct tttgcagaaa tcaaagtcga acttgagcct tcgcaagtca caatgggaga
     7081 aacctttaaa ttatccatta ctcaagaaga tcaacagggg ggcggagttc ccgatctgac
     7141 tgaattgcaa aaggattttg tgattttggg tacagaaagg catgtaaact attccattat
     7201 taatgggcag gcgcaatcac aaagtcaatg gattgtactc ttgagagcgc taaagccagg
     7261 tatttttact attcctccaa taaaagtagg ccttgaacaa agtctgccaa tcactatcaa
     7321 tgtccagggt agtgccaata cacaagcttt gccagatgaa gctatacaaa agcaggacgt
     7381 tttcctgaca gctgaagtga atgagaaaaa cccctatgtt aaccaacaag ttatttatac
     7441 ggtcaaatta tacaatagca aacgattgtt agatgccgat tatcaaggtc caaaagttga
     7501 tgatgccttg ctgattccgt taggagatgc gaaacgttac catgctgttc ataacaacat
     7561 taattatgtt gttgaagagc aaacttatgc gatatatccg caaaaaagtg gcactttgaa
     7621 aatcatatca ccgattttta atgctcttgt atatgactat aacccccaac gcaccaaagc
     7681 gacggataaa gcgattagct taactatcaa gccaatccct aaaaagtatc aaggcaaatt
     7741 atggttgcca gcaaaacaag ttaagttatc cgagcaatat gaaaacagta atcagaccct
     7801 cagtcaggga agtacgttaa cacgtacagt gacattggaa ggaataggca ttccagctca
     7861 gttattgcca acacttacct ttcaagaaac agatttcttt agcgtttatc cagagaaagg
     7921 ggcagaacgc aatcaggtta agcaaggtga gctaattggc agtgtcgaaa tcaaggtcac
     7981 ttatttgttc aacaaagcag gcaaaacgac aatacccgaa ttgcgtcttc cctggtttaa
     8041 tactgaaaca ggtaaagaag aaatcgctgt tttacctcca aggagcatag atatcatccc
     8101 ttctactgcg gtcaaggcaa atactgcaaa cactcaagtc acaggtaaag aaatgtctgc
     8161 cagcagtcaa tcagctccag attctgcatc agttgccaat gaatcaggag gttgggctgt
     8221 gaagctggca atattttttg gtttggcctg gctaatcact ttaagcttat ggatatggca
     8281 aaagcgaccg agagagtcat ccagtaaaaa ggagtataaa aaagctttgg atgctttaaa
     8341 taaagcttgt agccaatgta atcccaaaaa agctcgtgat gctgttttaa aatgggcctc
     8401 tttgcaatgg cctgatgctc caatgcttaa tttaactgac ttggcgcaat tggtaagaga
     8461 cgcgcattta aggaaacaat tgcatctgct ttcgcaagtc ttgtaccgaa gtcaggaaaa
     8521 aacattatgg cgcggggatg agttattacg ggctgtgcaa gcggtaaaac gtaacgaatc
     8581 tgtgaaaaag cgaaaaaaca gtaatttacc tcctattaat ccattttaga aattaaatct
     8641 atttaagcga tggtttttac tagttattcc tttactgtca atacggacaa cttatttatc
     8701 ctcttttttc tgtaatcacg ggactattgc cagattgtat gccgtcataa tttggctaag
     8761 ctcgctcagg cgactatgat aacaacagct ataatgttat agataacctt gtaaagagta
     8821 atgttctctc tttataaagc tctctggcta ttgtaggaat tgagtatgca tgttaatgag
     8881 tatatccact atgatgtgca ggagctcgct aaactgataa aaaaccatca ggttggtgct
     8941 gaggaagttt tgcggtgtgc gcaagaacgc ctatatcaag tgaatccttc attaaatgcc
     9001 gtagttacag attgttttga atatgcgcat caatgccttg cgaaaatgaa aggtaacgag
     9061 ccctattacg gtgttccgtt attagtcaag gatctgggcc atgccataga gggcattcga
     9121 ctaacggaag ggtcaaggtt ttttgcaaat aatctggctg agataaacag tgatttgatt
     9181 aataaattga ttgctcttgg ttttgtacca tttgcaaaaa caaatacttc agaattaggc
     9241 ctttcctgcg ttactgactc taatttattt gggccttgca gaaatccttt tgatactatg
     9301 agaacggcag gcggatcttc tggtggttct gctgctgcca taatggcagg gatagcgcct
     9361 gtggctacgg caagcgatgg aggcgggtcg attcgtattc cagcggcttg ttgtggtttg
     9421 tttggattca aaccgacacc aggtctggtt ccctcagggc catgggttga tgaagcatgg
     9481 tccgggatgg cgaccagttt tatactcact cgttccatga gtgatgctat tgcattgttt
     9541 aaaaatgtaa taaaccccat gcgacttaac agactatcga gaaagaaaaa aaaattgctt
     9601 attaccgagt tgacaggcgc ttttgccgag gtaccaattg caagggaatg ccaggaggcg
     9661 gtagagaaaa taaaagaaat cctgaaaaat tcaggccaca gattaaatga agtgcacttg
     9721 ccattggatc tgcaagttat cagttcatgc gcccatactt taattgcagc gagcacttat
     9781 gcgaaagtaa aaactcaaga acaaatcagt ggcagaaaac caatgattca tgaattggaa
     9841 cccataacat gggaatttta ttacagagga aaaatggtct ccgcttatga attcattatt
     9901 gccaaaaaca agctatacca atcactaaga cctctgcatc aattattgga aaaaacagac
     9961 attgtattaa ctcctgcatt ggctcaacta cccctgttaa tcggtcattt gcgaattgat
    10021 gaggaattca ataagtatct acaaaaaaag aaagaatttt caccttttac ttccctgttt
    10081 aatcaaggtg gattacccgc tatgactgta ccagttatgt tccaccatca tttgcctgtc
    10141 tctgtgcaaa tgggttcagc aaaaggcaat gatttgctct tattggagtt ggctcatgaa
    10201 ttacagcctg tgctgccaaa aaatatcacg cctgcatcaa ttccttgatg ctgatttttt
    10261 gtaagttttc atttatcgaa ccttgtgtta agttaactgt agcttttcat aaaaataaga
    10321 ataagtgatt gttttttata gctatagtca cttgaatttt aaataatcga ttctctacgt
    10381 actgctgctt aacgcggttt ttaaaaatca agtcactata tatttttcca acttcagggc
    10441 attttgcgta ggcagtaaat agttacagtt tgagtcgttg tatttaataa aggtaagttg
    10501 taaatggatt tcactaaaga ggatttcaat gaagacgtta ccatccaaag tcatttttat
    10561 aggggcaggc ccgagcggct tattcgctgc acgtcaatta aaggaaatag ccaaattaca
    10621 aaataagaca gtagaatgta tatttcttga acgtgaaccc gtagtgggtg gcaaatgcca
    10681 cacctactcg gatgcagttc atcctgagtt aaaaacggag tggggagccg cattggttgc
    10741 tcccaactat ggtgttgttc ttgatgcatt agctgagcat gacattaaat ttgaaaaagt
    10801 aatgccaacc gcttctgaaa cgatagaaat caagcaatta tttgaaaaag ccagttcgtt
    10861 agaaaaaata agcatggaaa tgaagttgct tagagaatta tgggctttta atagtgatta
    10921 tgatgtttat aaaaaggcaa aacaaaataa aaaagcactt ccagataaat tgttaaaccc
    10981 tttttctgaa tattgtaagc tgaacaacat gtcttattta cctgtctttt taaagccatt
    11041 tgtgcctgga tttggttacg gggatttaaa cttttgccca gcttattgtg ttcttgagta
    11101 tatgggtaaa gtcaccatgc ctaacatcct gttagccgat caattaattg gcaggccctc
    11161 actattagca atacatggcg gttttcagct tttaatggag agaatcgcgg aacaatttga
    11221 tgtgcgtacc agtgcttctg tttccaggat taagagagaa aaaaataagg tgactgtgac
    11281 ttatgtgcat aatggtatcg agcaaacaga aacagccgat actttggttt tggcgacttc
    11341 accaaaaaac tggaaatccc tgggtttaga cttaactgaa gttgaaaaca attgtataga
    11401 gagtctggat tattatcgct atccggttgc tgtttataaa atcaaaggat tgccgccgca
    11461 acagtatttt ttcacaaaag cacttgagga aaggggcttt gggcatttgg cgcttattac
    11521 aaccagggat aacaggagta atccggaaga tggacggtta tgtacggttt atgtgaattt
    11581 atctcccaat aataatgaat ttaaatttga tcatgaagcg ctaagaaaag aattagagtc
    11641 tatcccggat gtaactgaag ttacggttgt aaaggataaa atctgggaag attatatgtc
    11701 gactcttccc tgggacttgc gattgcaatt ggataaagag caacagcgca cgaatactat
    11761 gtatttgggc tcttatgctc tgggaagttt tgaagacgta gcctgtgtgg caaataaggc
    11821 aaccgatgcc atgacggaat ggtatgctcc cactatgagg tatgaagagg atttcagctg
    11881 gaaaaatatg aatagggcct ggcagttttt tacatcgcca acgaaatcgc cactcgcttc
    11941 tttgggtgag cccaggatgt cggatgaaca cagtaacagg ccatagacgg taagataaaa
    12001 gtggtacatt tatcgaagat attgtacata atgctcgcta tttgtagaat agtgtattat
    12061 aatataatca cttgttctac gagcattatt ggttaaaatc ttgtttttcg acaaactcct
    12121 tccttatatt tcccttggtc gtgaatttct ctcgatgatt acattgtatt ttttctctta
    12181 tcgctcatta caagtgtttg tttaatttta tttggaactt tgaaatgtct gtgaaagaaa
    12241 ctggccacgt taagtggttt aatgatgata aaggttatgg tttcattagt cgtgataatg
    12301 gtcaagacga tgtatttgta cacttccgct ctatcgttag cagcgctggg cgcaaaactt
    12361 tgctagaggg acaacgagtg gaatttcttg ttactaaggg agcgaaaggt ttgcaagcag
    12421 aagaagtaac tgctatataa tcccgtattc actgaattac cttcctcttt gtctcaaaac
    12481 aacgaagagg gaggtggttt tttaatgcca ataaattgca ttaatttttt caattatttt
    12541 tcactttctc ttaagaattt gtactgagac ctgtttggta taaaaactgg cagtaagata
    12601 acgatattcc actttatttt cttttaccca ttcttggaaa gctttaaatt catcctggcg
    12661 ccaattgata ttaccaatgt actcatcgaa tactatgata gtacctggtg caatctggga
    12721 gtttaattcg ttgaacacca atttagtgga agaatagaga tcacaatcga tattcataaa
    12781 agctatgggt tccctgttat tttttttaaa atctggcaaa gtttcattaa accatccagc
    12841 gattaaagtc acattacttg ctacggaggg aattttgccg taggtggaat aggagccagc
    12901 cttttcgtgg taccaatctt caggtaaccc ttgaaaactg tcaaaaccga aaattttgcg
    12961 tttgggaaag agtttggcaa tatgggaaat ggaatagcca aagcgcactc caaattccag
    13021 tattaagcct tcagaaagaa cctgtggcgc taataaatca aatgtttgat aagttgttcc
    13081 actatgtcgc aaatgtttgt cagcatgttt atgaagataa tcagctgcat caatgagatc
    13141 aaagcgtttg aatgcgcgat acgtttctat cggtgtcttg tttaaaccat tgagtatgtc
    13201 tattgcaatt cgctggcagc aaaaataatc attattgggt atgcgttgaa ttgctctgtc
    13261 taaaagagat tttactgctt ttttatcatt taaagccaga tataccgcgt gcagtaaaat
    13321 ggtgatgtgt ccatccttat ccgttttttc aagtggttca agtaagaagc gcgcttcctc
    13381 tactttatta agatcaagca aaataatggc tcttgcccaa cttgttttgg ggatattgaa
    13441 ttggtttaca taagggaata aggcatacgc ttcttgtgcc cttccttgtt gccttagagc
    13501 atagaggtaa tatgcgaggc tttcaccgtc catttcttca attttaaatg attcaaaaag
    13561 cttgcaggct gacaaagaat aattagcatg cgccagagtt tttgctgtca ttaatcctat
    13621 gtcagttttg aattcgctga ataaataagc ttgccagtac atggctgctg cttctatggc
    13681 tttacccatg cgaagtaaaa cggttccttt ttgataatag gctccggaaa attgtgggtt
    13741 tatcgctaat gcttcatcat acagtgttaa ggcaagaggc aaatcgccct tctctaaagc
    13801 ctggtctccc ttggatttaa ttacaacagg atcttgttgg gtcatttatt ttctccagta
    13861 cctacttcta tatcatagta ttcttttatt agtaatgtag ttgtattaaa aaaatcctta
    13921 ttaatcaatt tcatgaaaaa ataattgctg gcattcatat ccgactaggg tataatgggc
    13981 ttaccaaaga caagccagga tgtcgtaatg ataaaaccaa tcaccctgct tttgcttttt
    14041 tgttctttca ctgttaatgc ctcaattctc gtcaatggta ctttcgaagc aacaaaatca
    14101 tgccctgctt acctttccaa gaatcgtaaa tcaaatcctg ataatttaat ggttgagccc
    14161 aaccagaaat atcaattaaa agaaattaat aaaaccaaac cggattggtt gcgtatacaa
    14221 ttttcagacc atcaacctaa tctgcgctgg gtgagtgttg attgcggggt cattgaatac
    14281 actgagcgtc ccaaagcgat ctgtgatctt tctcccggga tggctgattc ttatgtgttg
    14341 gctttaagtt cacagccggg tttttgtgaa acttatggat atgaaatagg caaaccagag
    14401 tgtttgaaat taacaaagac agcttatcag gccaatcatc tgatcctgca tggattatgg
    14461 cctaatcaag aagcatgcgg ccataattat ggctattgtg gaataagccc tcgaacaaac
    14521 cattgtgatt attctccatt ggatttgtct tctgatgttg ctcaaaattt aaagaaactt
    14581 atgccgagtt accattatgg cagttgtctg gagcgccatg aatggaacaa acatgggagc
    14641 tgtcaggatt taagcacaga tggttatttt agtttggcca tgcgcttaac tacagaagtt
    14701 aataatacag agttcggtca atatcttacc gctcatcgtg gtgatactgt aaaattgact
    14761 actttgcgtg aattgattca aagatctttc ggggaaaaca atacaggtaa agtgacttta
    14821 agttgcaaga atgggatctt ggtggatatt tatgtccaat tacctgctct catacctttt
    14881 aaagagtctc tagagtcttt ggttgataaa gccagtcact cacaacacca tgattattgt
    14941 aatagcaatg tcaccatttc tgattttagt aatgaaaaat ggttataggt attcaagtgg
    15001 aggagatgac tcgttcacaa aactttcttt aaaataatat ggcattgctt ccttatagga
    15061 gactgccttg cacattgtca aaacttcctc taatgtagag agattattga tataggaata
    15121 cagattatgc taaagcggtg ctacttattg attttactta tgtttgtatt ggcaagctgc
    15181 gcccatcata aaccgcaaac acctccagcg gaggttaaaa aacagggtac cagcagtaca
    15241 cggcagtttc gccaagtgtc atcgttcaat caaatcgtag ttcagggacg actgaatgtt
    15301 aatttgcata ccgggtataa taagccagag gtcatgttac gcggtgatcc cagagatttg
    15361 gttcaagtaa gaacggtagt aaaacaaaat acactttatg tgtctttggg ccagggatac
    15421 ccggattatg gtgttgtgac tgtggatatt aaaaccaaat tcctcaaccg gtttcgctat
    15481 gagggtgctg gggttgtgac agggaataat atacggacca gctatttgga tttgtatttg
    15541 gcgaacgaag gcaccaccag actggcagga aacattgggt tgcaaaaact ggaagcagtc
    15601 ggtagtggtg tgacacaaat caatggtgta tccagcagga atttgcaaat cgtattgaaa
    15661 ggagatccca aagtactgat atctggtttt gtcaatctaa gacagcttga tatgtatgga
    15721 aaaggcacgt taagtttgta ttggataaaa agcgatactt taaccattag agcaaaaaaa
    15781 gccgccaaaa ttcaattggc tggaattgtg aatcgcctgg atgtcgaatt gtgggatttt
    15841 gctcaattta aggggaaata tttacgagcc caacgtagtt ttgtgaaaac tcatgataaa
    15901 tccgtagcag aaatttcagc tgtgaaccat caaagcagcc ttgctaccga tgccagtgat
    15961 atttactatt ataacttatc aaaaaccagg gctgatttca tggcatttaa tgggtccgtc
    16021 ttggatatgc gcgaatggag ccaatctgat ctaaaagatt ttgatcgcta caataagcag
    16081 tttccctagc attgtaactg ttcatggggt atgtaattaa ggaatattag gtcctctgtc
    16141 tttcccgcgc aggcgctgag tgatcctgtc attattttac tcagctcaaa gcgccacatt
    16201 caataaatga gtgatattgc tgtttcaaat cactcattat gtattgaaat aaatcccaat
    16261 tcagtaattc cggctcatgt cgataagcca ttctagcaag atgaataaaa gagcgaactc
    16321 gtttgttttt tatagtattt gcttgataag agcgatgttt attaagggat tcaatcatta
    16381 aaccgaacca ccaggcaatc ataattaata aaacagcaag aaaaaaaagc atattaactc
    16441 tggtttttcc tgaagattga tttcgtcgta atcccattcc cagttgttca ttttttatgt
    16501 ctttgaaatt ttgttcgatt tgcattcttt ttttgtaata agacacgaga aatcgagcat
    16561 gcttatttaa tgagctaaag agaacccatc catttttata ataattggaa tgagctttat
    16621 catgagaagg atatttgttt ttcttttttc gtaccttgcc tgagagtttt tctttatagg
    16681 tataaagata accttccacc gccttttttg tttttccaag cttgacctta cctaatgcgg
    16741 aagcttttcc aataccttca aaaagaatat ctttcacttt gtgccaattt gtttctcctt
    16801 caatctgata gcaatacaaa gaatagatgc ggccaataac atcccagcct agctcaagaa
    16861 cctgttgaaa ccactcacga tgaaaacccg cgtctgtaat aattgtcacg gacaaactcg
    16921 aaggaataac ctctttcaag cgtaataaaa attgacgatg aatctctgca ttttcttgct
    16981 ctgactcaga atgaacttcg tgatagattg gtattgagcg accatgagct gcaatactag
    17041 cttccagaac atgatagccc tcgctacatc ctccagtcca atcaattaaa acaacaat
//
LOCUS       DATNSN010000061        16852 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.061, whole genome shotgun sequence.
ACCESSION   DATNSN010000061 DATNSN010000000
VERSION     DATNSN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16852
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.061"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            132..635
                     /locus_tag="VAH76_001618"
     CDS             132..635
                     /locus_tag="VAH76_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386246.1"
                     /translation="MSIFKTIKNIIFNPNVYIAVVIGALLGGVSGGAVGLFSGGFIGR
                     SFKICMDCPNQLLGFNIGIFDLNMVAGAIIGVVIGAALGGAITGLITTFHVYTKPHLP
                     KTVSRDNIHEVLISALWISIEISLGIILGAVIGSLKSPGIGSAVGAFIGIILMLLTAI
                     WENRAKK"
     gene            complement(684..1178)
                     /locus_tag="VAH76_001619"
     CDS             complement(684..1178)
                     /locus_tag="VAH76_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEO1386247.1"
                     /translation="MGSLRKHQPGKTTDLLMSPFLRLQQEVNHAMSDFYNLFDPQRSA
                     NLEQFEHISLTPSLDIVEDKDNFKIEVEMPGMGEEDIKVSFCENRLTIEGEKTTSKKD
                     ENKNYISREISYGRYERTISLPLSADVDKATASFKKGMLWITIPKKTEAKENVKTIKI
                     EKAK"
     gene            complement(1344..2009)
                     /locus_tag="VAH76_001620"
     CDS             complement(1344..2009)
                     /locus_tag="VAH76_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEO1386248.1"
                     /translation="MNALMTNVEHPVQIPSGHYFLNGLLYIPENAQGIVLFVHGSGSS
                     RFSVRNQFVAQSLNQACLATLLFDLLTPDEENIDNYTREYRFNIPLLASRLIDATHWC
                     MKELQSFHLQIGYFGASTGAGAALVAAGQIPDRVKAIVSRGGRPDLARTYLSLVVAPT
                     LLIVGGYDEVVIELNRQALTKLNCDKRLEIVPGATHLFEEPGALKEVARLAQNWFKQH
                     LKE"
     gene            complement(2090..3076)
                     /locus_tag="VAH76_001621"
     CDS             complement(2090..3076)
                     /locus_tag="VAH76_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO1386249.1"
                     /translation="MHAMIMEKPGCALQYQEVNKPNPNENELLIKVSACGICRTDLHV
                     VDGELKNPKIPLIPGHQIVGTVEQVGSAVKQFAIGQRVGVPWLGGSCGICNFCTSGRE
                     NLCDQACFTGYQIDGGFAEFCVANYHFCFPIPDGYPDHQAAPLFCAGLIGYRALLKVG
                     NATHLGLYGFGAAAHILIQVACQKGIKIYAFTSPGDQESQKFAYRLGAVWAGDSTQSP
                     PHLLDAAIIFAPVGALVPQALRNIVKGGTVVCGGIHMSDIPSFPYEILWGERAVCSIA
                     NLTRKDGEEFLSLAPKIPVKTKVQTYPLTEVNRALDDLRHGRYRGAGVIVMK"
     gene            3207..3656
                     /locus_tag="VAH76_001622"
     CDS             3207..3656
                     /locus_tag="VAH76_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO1386250.1"
                     /translation="MRIGEFCNRDVVIINGDESVKQAAELMRTHHVGDIVLVEELKGH
                     RVPIGIVTDRDLVVEVMALDVNPEELAVQDIITRSVLVAREEDSLIDSLEFMKEKGVR
                     RLPVVDNDHELVGIITIDDITELLAEMLHKVVGVVDRQQKIEKQQRS"
     gene            3737..4300
                     /gene="thpR"
                     /locus_tag="VAH76_001623"
     CDS             3737..4300
                     /gene="thpR"
                     /locus_tag="VAH76_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEO1386251.1"
                     /translation="MNAIRAFFAISLPPEIQDNIRGNLEPLRQMAPVHSIRWTKPMHL
                     HITLQFLQRLETEHIQVLIERVSNELNHIKQFDLEFGVLEWFPSPKNPRLISLSVDPQ
                     DRLRDWSRMIGQVMDSLKYPLEKRPFRGHLTVGRVVNFSPKLQQLLQMKFPLIPPVPI
                     NKFYLIESRPDKEGSNYFPLAQFDLTR"
     gene            complement(4424..8308)
                     /gene="lepB"
                     /locus_tag="VAH76_001624"
     CDS             complement(4424..8308)
                     /gene="lepB"
                     /locus_tag="VAH76_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS GTPase-activating effector LepB"
                     /protein_id="HEO1386252.1"
                     /translation="MLTYQGKEIVRFKEKTGGKNKSDVDGFYEDSDGRKFFIKKPGDP
                     RELFTELFAGLLLKEFMKRGLIDESYFPSLICADVIQFEDKSYGLIQPLVSFDELHKI
                     IGTSYGDGSDRNTIKETFFGPGYYAGITKQNKYFGLSMALMFSLLLGAHSVHSGNIVV
                     LKGEGEEKSKQFGRIDWGDAFRYFAHPNNNDNLLYAYENRGWFNYKSLTKDYFLNYKK
                     INGLFPAMAEKARQLQSKLNPELLIDIVTGALKNIPTDLIEEKTKIQLAAYMCMDSLK
                     EATFGPEGNCKSFAIAMAALLENRLGKIAVLKDMSPLSNPEELYQSILELKYMTLPMT
                     SSTSFSETINQWTNILKTSDMEKFSFDSNPINLLELVKQFNLYVDELAITCEANNVWA
                     KERIDSTPNLFALYDNSGGEAIHGHAFVPYYKESIVLRRLFTVDPNTFNLSRFAAFEG
                     PCQLYCKEHKDSAWVKIQTLLTLGNGIINTLKIIKQAQAFGIDEAVTENLKALKEQFI
                     AFQLAEADVKESLKAPTFAEPLPDKESEFFYPIDEKALAKMNGYQLATICLEELNSPK
                     PSPLIERILSNKKFWKRINSAFESGVFKGRTDDPAGKIAKIREWHQLLQISGKKTAGQ
                     IDELQKIVISLQSKIQKQTIEFEELEATLIQIKEKYQLQEKMAEQSEHEKSSAQSIIR
                     SLNLELSQLKLQLQEQEKLQFQLKELKEKIHEQTTLSKRLGEKLQTQKKTNTHQEETI
                     QRITKEKSLADSSLESLRKELHELAKKESSLHKTLEEKQLQVQQLEEQLAEKEKENLV
                     LKKADKQSQHEKSLDKSAIESLTSELNQLKLELQKQEALQLQLKSLSEQVQEQTLLVE
                     SLKEELQKQKESNTHQEETIERITKEKSLANSALESLRKEMHELTRKNEENQLKLIKQ
                     VHSLSEQLEEKQLQIREFEKQLQEKEKRVEQSEKEKASEKRTVVSLREQVSNLKLQLQ
                     QLGEVIQEKEKGSSLISQQSKQIIALQEIIEDQKRQLEELKIKIQELASANQELGKQN
                     QSLSKENLHNKNTVEDLKKKLNELSVQLEQLHQSSSEKEQTIRKLREELIKKDSSLKQ
                     NEEMQLVQKHLQEEIDRLQKEIKQQQLNVNQLQSLIAQSKEAEKRYQQALQQKKGIYF
                     ARMERVSPIYWQIQQIEQKAKELEERRETEASTAAKTLATKLRLEIKNYLDNSEFDEK
                     SALNSFKINAKRHIENSKETLNQHREEWKYLLANVTLGVFLLGIGYLAAILINKATTG
                     NYTFFSQTNSGKKLDALEKAISSTHSETLVYG"
     gene            complement(8388..8624)
                     /locus_tag="VAH76_001625"
     CDS             complement(8388..8624)
                     /locus_tag="VAH76_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386253.1"
                     /translation="MLQPFITDFIKNRSFQFIFVMGLVSDIALAGTIKHIVFLFGFPY
                     LSSVGLYCLMKRKKTTVKARISEQYRPKQKEICR"
     gene            complement(8660..9343)
                     /gene="radC"
                     /locus_tag="VAH76_001626"
     CDS             complement(8660..9343)
                     /gene="radC"
                     /locus_tag="VAH76_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO1386254.1"
                     /translation="MMVAHTAQQLDLREKLLTNGVHSLSDIELLAVFISSGNNKKSCL
                     QLAYELTKHLGNLRNILNADLQSFKSIHGLGEVRYAQLQAAKEICHRSDFIDLQKEIQ
                     LSNTQQTYAFLKKRLRDYKNETFAALFLDSQHRIIAYEELFSGTINTATVYPRPIVER
                     VLQLNAAALILAHNHPSGLSDASQQDFAITERIRDALDLVDARLLDHIVIGDNEVYSI
                     FAENKWVCN"
     gene            9420..10616
                     /gene="coaBC"
                     /locus_tag="VAH76_001627"
     CDS             9420..10616
                     /gene="coaBC"
                     /locus_tag="VAH76_001627"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEO1386255.1"
                     /translation="MQDFINKKILLGVCGGIAAYKSAHLVRELTRLGADVRVVMTHSA
                     QQFINPLLMQALSGNEVRIDLFDTQAERAMGHIELARWADYLLIAPASANCLAKMAQG
                     LADDLLSTLYLVTETPVIVCPAMNRSMWAHPATKANCELLKERGVFFVGPEEGSQACG
                     EHGLGRVSEADQIVNTLRLLDVNQLLHGKRIVITAGPTRESIDPVRYLSNYSSGKMGF
                     AMAEAAVMAGAQVTLISGPCHLQTFSDIELIRVESAQSMFDEVNRHLQQGDLFIGTAA
                     VSDYSVLNPASEKMKKNENSELTLQLVRNTDILSEVAKSGKASFVVGFAAETTDVISY
                     AKEKMKNKKLDMIIANQVGKGMGFESDVNQVTVITKNKQIELPLTHKTRLAGQIIAIL
                     AASLQN"
     gene            10643..11101
                     /gene="dut"
                     /locus_tag="VAH76_001628"
     CDS             10643..11101
                     /gene="dut"
                     /locus_tag="VAH76_001628"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO1386256.1"
                     /translation="MHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVA
                     PQQAVLLPTGIAIYIADPKLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKISCW
                     NRSQEHFTVNPGDRIAQLVFIPVVQASFEVVNEFTESSRGEGGFGSSGRY"
     gene            11102..12490
                     /locus_tag="VAH76_001629"
     CDS             11102..12490
                     /locus_tag="VAH76_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEO1386257.1"
                     /translation="MTYQQKQIPRSVFRAYDIRGIIGKELDENSFYSIGLAIARYLQK
                     LNRRQIFVARDGRLTSYALAKALKEGLLDSGIDILDLGAVATPVMYYATHTQGIDSGL
                     MVTGSHNPADYNGIKLVLAGKTLIQDEIDTLYALLGEVKQSAVRGKETAYAVIEDYIQ
                     RIVSDIQLKRPLKVVVDCGNGVAGPVIPRAISKLGCEVIPLYCEVDGRFPNHHPDPSI
                     EANLVDLKAAVANHQADIGLGFDGDADRLGLVTNKGEVIWPDRLMMFYSREILSRNPG
                     ATVVFDVKCSSHLEKEIQAAGGVARMCPTGHSIVKAVMKKEQAILAGEMSGHLFFKDR
                     WYGFDDALYSACRLLEIISSSHLTVSEQFELIPNSVNTPEIKIAITDEEKFDFIQRFS
                     EQADFPDGRILNIDGLRVEFPNGWGLLRASNTTPCLVARFEATDKDHLEQIQYLFKKQ
                     IQRLDSELDLPF"
     gene            complement(12596..14329)
                     /locus_tag="VAH76_001630"
     CDS             complement(12596..14329)
                     /locus_tag="VAH76_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO1386258.1"
                     /translation="MNSEVDTLFAQAYKLQYEGHLPQAISLYEQILAQSPKHTETLHF
                     LGLTYAQLGDMENAILYFLQARKINPNDVGILNNLANAYKKAGQLDEAIKYYQQAIEI
                     KPEYVQAHNNLAATYALLNNYQKALHHYVIAVNTEPDFSAAHFNLGLLLLKNQQLSAA
                     KTQFNNVIALNPQHREAQFYLGILHLEDNLLSEAEQAFHKVLEQDHEHVQSLINLGVI
                     ALKREQNQLAVDYFTKALALDNEDIDARNNLAATFMHHDRFENALMHYDVLLKKEPDN
                     LEYLYNSGVAQMALGHLNEATLLFDQILTLQSDHTPSLNNLAAIYLKMDMRETAREYL
                     ERALVINPDDVVSRHMLNAITGATSVNTTEKYAQNLFNNYALYYDQHMQGELHYKIPH
                     HIGRLIHQLQLLQTSHSLDLGCGTGLTGIVLREISKHLTGVDIAEKMIARAKEKNIYD
                     LLVCSELIDFLRKDKNDYDLAVAADVLPYFGNLDDIFNSINQHLKQEGYFIFTTEIST
                     TTPWKLESSARFSHQPEYIESLINKYQFHLIKQEKIPGRTQNKQVLEVMLYCVKKSTS
                     HFVSNRTYETP"
     gene            complement(14331..14915)
                     /gene="rdgB"
                     /locus_tag="VAH76_001631"
     CDS             complement(14331..14915)
                     /gene="rdgB"
                     /locus_tag="VAH76_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEO1386259.1"
                     /translation="MKEIILATSNPGKIKELEQLLAPTICIPQADLGISDAEETGLSF
                     IENAILKARHASSLANKPALADDSGLVVPSLNGEPGIYSARYAGIKANDEDNIQQLLS
                     KMADLSQEQRQAYFFCAIALMQHAKDPTPLIATGVFHGVISMKPSGTNGFGYDPVFYL
                     NEYQCTAAELPAKIKNRISHRAKALNQLRALLPD"
     gene            14964..15521
                     /locus_tag="VAH76_001632"
     CDS             14964..15521
                     /locus_tag="VAH76_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO1386260.1"
                     /translation="MRILEKNIKKTPWYLKPFFWNQKRKYGQILEPALIWARIPRLFL
                     AVSAMYGVIDRKKSPLSPILRSLITVRVSQINWCEFCVDINSATLIKRAGSEAHYKQL
                     AKWRSSSLFSPEEKAALDYAEKVTYSDQRPDDECFNQLKLYYSEQEIIELTALIAFQN
                     MSSKFNSALGVEAQGFCSRQLTGKS"
     gene            complement(15548..>16852)
                     /gene="sdbB"
                     /locus_tag="VAH76_001633"
     CDS             complement(15548..>16852)
                     /gene="sdbB"
                     /locus_tag="VAH76_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbB"
                     /protein_id="HEO1386261.1"
                     /translation="LQQFVANSLFPAGAGDWYKNKGYGKKGEVDDEAPFRDFVEKQKT
                     DKKSHYYKEFQGLDLSLKKVKTKLVSGESCQLEVMKCQPESNEPKKPGTGKHIVYFPG
                     ANTYYQACFRDISTACKETGATVHAFNFPGTGLSSGKVREANDLINAGLSVVSSLIKQ
                     GVHPDDIILQGDCYGASIALEVKRQLEEQADIKVRAIMNNVFKSFKAAVCDMITQSPW
                     LPNILKSIVKRLLEFTGWHVTPGKKYKHADPYQCHIQHLGDQTLESSTLSSKVSKYQH
                     EIQTGQTKSQKRAPITDTCPEEYRKDRDELDRKHYVRVKETAKERLASKFGVDKFGRV
                     NAHFADLCELEMLDGQSVYQGFVNDYIARSNAYIEKHPQKGIKDIQQDLSQLHYLQAA
                     DSIEITEDEAQDFNTVVDLITDEQQLRHDRFNDNTIGKSISM"
BASE COUNT         4710 a         3092 c         3390 g         5660 t
ORIGIN      
        1 aacgatttta ttcaaatttt acaataatag agctttgctg cagtgttcag catttgatac
       61 agcttgtgac ataaaagaaa catgagctat gctattgaat tatttaacta actcaaaaat
      121 gccatttgaa tatgagtatc tttaagacaa taaagaacat tatatttaac cctaatgtct
      181 acattgctgt ggtaattggg gcactacttg gaggggtaag tggtggcgct gtcgggctat
      241 tttccggtgg attcattggc cgctcgttta aaatatgtat ggactgccct aatcagttat
      301 taggttttaa tataggtatt tttgatctca atatggtagc gggtgctatt attggagtgg
      361 ttataggggc tgctttgggt ggggcgatta ctggtttgat tactaccttt catgtttaca
      421 caaagcctca tttacctaag acagtttctc gcgataacat acatgaagtg ttaataagtg
      481 cattatggat tagtattgaa atatcccttg gaattatttt aggtgctgtc ataggaagtt
      541 taaaatcacc gggtattggg tcagcggtag gcgctttcat aggtataatt cttatgttat
      601 taactgccat ttgggaaaat agagccaaaa aataagaacg gggctgactc catcgcgttg
      661 gagcctgacc ctgactgaat taattatttt gctttttcaa ttttaattgt ttttacattt
      721 tccttggctt cagttttttt aggtattgtg atccataaca tacccttttt aaaagaggca
      781 gtcgctttat ccacatccgc agatagaggt aaggaaatgg tgcgttcata tcgcccataa
      841 ctaatttcac gactgatata atttttattt tcatcttttt tagaagttgt tttctctcct
      901 tcaatggtta atctgttttc gcaaaaagat actttaatat cttcttcacc cataccaggc
      961 atttctactt caattttgaa attatcttta tcttcaacga tatccaaaga cggagtaaga
     1021 gaaatatgct cgaattgctc aagattggca cttctttgag ggtcaaataa attataaaaa
     1081 tcactcatag catgattgac ctcttgctga agtcttaaaa acggactcat cagaagatca
     1141 gttgttttac ctggttgatg ttttcttaag ctgcccatga tgtcacctcc ttattccata
     1201 aattgacgta tcagttagtt gttaaaaaca ccgaaagtaa tattcaccag cttatcaatg
     1261 taaatatagt tgctagataa aatattttca attgttataa atttatttca taatgatttt
     1321 atattgttgt ttttgtattc gttttattct tttaaatgtt gcttaaacca attttgagcg
     1381 aggcgagcta cttccttcaa agcaccgggc tcttcaaata aatgagttgc accggggact
     1441 atttctaacc gtttatcgca gttcagtttt gtcagggctt gtcggtttag ttcaatgacg
     1501 acctcatcat atccgcctac aatcaacagt gtaggggcaa caaccaatga taaatacgtt
     1561 cttgccaaat cagggcgccc tcctcgggat acgattgctt tgactctatc cggtatttgc
     1621 ccagccgcaa ccaaagcagc ccctgctcct gtgcttgcac caaaataacc aatttgcaag
     1681 tggaaagatt gaagctcctt catacaccag tgagttgcat cgatgaggcg cgatgcaagc
     1741 agggggatat taaagcgata ttcacgagtg taattatcga tgttttcttc atccggggtt
     1801 aataaatcaa ataataaggt agccagacaa gcttgattca aagattgcgc aacaaattgg
     1861 ttgcggacac taaatcgact gcttccactg ccgtgaacaa acaacactat cccttgagca
     1921 ttctcaggaa tatagagcaa gccattaaga aaataatggc cacttggaat ttggacaggg
     1981 tgttcaacat tggtcattag agcgttcatg atttgaatat tccttgctat caatcagtcc
     2041 ttcccccaat gatttgagtt tgttgtaata gcttttgata attatagctt tatttcataa
     2101 caataacccc ggctcctcta tacctgccat gtcttaaatc atccagcgct cgattaactt
     2161 cggtaagggg ataggtttga acttttgtct taaccgggat tttaggggcc agagacagga
     2221 actcttcccc atctttgcga gttagattgg caattgaaca aacagcgcgt tcaccccaca
     2281 gtatttcata agggaagctg ggaatatccg acatatgtat accaccacaa accacagtac
     2341 ctccttttac aatattgcga agagcctgag gcactaatgc accaacgggt gcaaaaatga
     2401 ttgctgcatc aagaagatga ggtggtgatt gcgtagaatc cccagcccag acagcaccta
     2461 atcggtatgc aaatttttga gattcctgat caccggggct tgtaaacgca taaattttaa
     2521 ttcctttttg gcaagcgact tgaattagta tatgtgctgc cgctccaaat ccatatagac
     2581 ctaaatgagt tgcattacca acctttaaca aggcacgata tccaattaaa cccgcacaaa
     2641 ataatggtgc tgcctgatga tccgggtatc catcgggaat aggaaagcaa aaatggtaat
     2701 ttgccacaca gaactcagcg aaaccaccat ctatttgata tccagtaaag caggcttgat
     2761 cacataaatt ttctcggcca gatgtgcaaa aattacagat cccgcaactt ccacctagcc
     2821 aagggactcc aactcgctgt ccaattgcaa attgcttcac agcggatcca acctgttcaa
     2881 cagttcctac aatttggtgg cctggaataa ggggaatctt gggatttttc agctcaccat
     2941 cgacaacatg caagtcagtt ctacaaatcc cacaagctga tactttaatt aaaagttcat
     3001 tttcatttgg atttggttta ttgacttcct gatattgcaa tgcacatcca ggcttttcca
     3061 tgatcatggc atgcatgggt attggtcctg tttttatcct ctatcttaca attatagtag
     3121 ggtgggttat acttacttaa tgtattcaaa aagtaacaat atttgacatc aatgatgttg
     3181 attgaatagt ttgcctggag gccattatgc gaattggaga gttctgtaac agggatgtgg
     3241 taataattaa tggtgatgag tctgtaaaac aagctgctga actgatgcgt acccatcatg
     3301 ttggcgatat cgttttagta gaagagctca aaggtcatcg ggttcctatt gggattgtta
     3361 cagatagaga tttggtagta gaagtcatgg cattagacgt taatcccgaa gaattagctg
     3421 ttcaagacat aattacccga tccgttttgg tggccagaga agaggatagc ctcattgatt
     3481 cattagaatt tatgaaggaa aaaggggttc gccgattgcc tgttgtcgat aatgatcatg
     3541 agctcgttgg tattattacc atagatgata taacggaatt actggcagag atgttacaca
     3601 aagtagtggg tgtcgttgac aggcagcaaa aaattgaaaa acaacagcga tcttgataat
     3661 taatttggaa aaagcgcttg tttttcggaa tttattttgt gaattgcaat atttttccca
     3721 ataggcaacg aattttatga acgctatacg tgcttttttt gctatttcac taccaccgga
     3781 gatccaggac aatatccggg gtaatttgga gccattgagg caaatggcgc cagttcactc
     3841 cataaggtgg acaaagccaa tgcatttgca tatcaccttg caatttttac agagactgga
     3901 aacagaacat attcaggtct tgattgaaag agtaagtaat gaacttaacc atataaagca
     3961 atttgatttg gagttcggtg ttttagagtg gttcccttct cccaaaaatc caagacttat
     4021 ttcattatcg gtagatcctc aagacagact tagggattgg tctcgaatga ttggtcaggt
     4081 gatggacagt ttaaagtatc ctttagaaaa acgtcctttt agggggcatt tgactgtagg
     4141 tagagttgtg aatttcagcc caaaacttca gcagttattg caaatgaaat ttcctctcat
     4201 tccaccagtt cccattaata aattttattt aattgaaagt aggcctgata aagaaggaag
     4261 taattatttt cccttggcgc aatttgattt aacccggtaa aagagaatgg ctgatttgcc
     4321 aattggaacg attctttgtc tctgtgtaca atttattatg ctcaagtgtc tttattattt
     4381 ggcataggat gaatttaagc caacctatgc caaaaagaga agattatcca taaactaatg
     4441 tttcggagtg agtggaagaa atcgcttttt ccaacgcgtc caattttttc ccgctgttag
     4501 tttggctgaa aaaggtataa ttgccagtag ttgctttgtt aatcaaaata gcggcgagat
     4561 aaccgattcc aagcaagaat acccctaaag taacattggc taacaggtac ttccattctt
     4621 cgcgatgttg atttagcgtt tccttcgagt tttctatatg ccgtttggca tttattttga
     4681 atgagtttaa ggcacttttt tcatcgaact cactattgtc gagataattc tttatctcta
     4741 aacgaagttt tgttgcgagt gtttttgccg ctgttgatgc ttctgtttct cttctttcct
     4801 ctaattcttt ggccttttgt tcaatttgct gtatttgcca ataaatagga gacactcttt
     4861 ccattcgcgc aaagtaaatt ccctttttct gttgtaaagc ttgctgatag cgtttttcag
     4921 cttccttact ctgagcaatt aaagactgta attgatttac atttaattgc tgttgtttaa
     4981 tttctttttg taaacgatca atttcctctt gcagatgttt ttgaactaat tgcatttcct
     5041 cattctgttt gagcgaagaa tcttttttaa tgagttcttc tctcaatttt ctaatggttt
     5101 gttctttttc acttgaagat tgatgcaatt gttccaattg aacacttaat tcattcaatt
     5161 ttttcttaag atcttcaact gtatttttat tatgtaaatt ttccttgctc aaactttgat
     5221 tttgttttcc aagctcttga tttgcagatg caagttcctg aatttttatt ttcaattctt
     5281 ccaattgtct tttttgatct tctataatct cttgaagagc aataatttgt ttggattgct
     5341 ggcttatcaa agaggagcct ttttcctttt cctggattac ctctccaagt tgctgtaatt
     5401 gcaattttaa attgcttact tgctccctaa gtgaaaccac agttcgtttt tcggaagctt
     5461 tctctttctc actttgctca actctttttt ctttttcttg taattgcttc tcaaattctc
     5521 taatttgtaa ttgtttttct tcaagttgtt cgctaagaga gtggacttgt tttattagtt
     5581 taagttgatt ttcttcgttt tttcgagtta attcatgcat ctcttttcga agcgattcaa
     5641 gagcagaatt ggccaatgat ttttctttag tgattctttc aatagtttct tcctggtgtg
     5701 tgttgctttc cttttgtttt tgtaactcct ctttcaagct ctctacaagt aatgtttgct
     5761 cttgaacttg ttcgcttaaa gattttaact gcaattgtaa tgcttcttgt ttttgcagtt
     5821 ctaatttaag ctgattaagc tctgaggtta gagattcaat agcagattta tccagggatt
     5881 tttcatgttg actttgtttg tcagcttttt tcaggactag attttccttt tctttctctg
     5941 ctaattgctc ttctaactgt tgtacttgta attgtttttc ttcgagcgtt ttatgaagag
     6001 agctttcttt ttttgccaat tcatgcagct cttttcgaag cgactcaagg gaagaatcgg
     6061 ccaatgattt ttctttggtg attctttgaa tggtttcttc ctggtgcgtg ttggttttct
     6121 tttgcgtttg cagcttctct cctaatcgct tggatagagt cgtttgttca tgtattttct
     6181 ctttgagttc ttttaactgg aactgtaatt tttcttgctc ttgcagttgt aatttaagct
     6241 gactcagttc taaattcaga gagcgaataa tggattgggc agaagatttc tcatgttcac
     6301 tttgctcagc catcttctct tgtaactgat atttttcctt tatttggatt aaagtcgctt
     6361 ctaactcctc aaattcaatc gtttgctttt gaatttttga ttgaagagaa attactatct
     6421 tttgcagctc atcaatttgt ccagcagttt ttttaccaga aatctgtaat aattgatgcc
     6481 attcacggat tttagctatt tttccggcgg gatcatctgt gcggccttta aatacaccac
     6541 tttcaaaagc agagtttata cgtttccaaa attttttatt actgagaatt ctttcaatta
     6601 aaggcgacgg tttagggcta tttagttcct ccagacaaat cgttgcaagt tgatatccat
     6661 tcattttagc taatgctttt tcatcaatgg gataaaaaaa ctcactttcc ttatcaggca
     6721 agggctctgc aaaagtaggt gcttttaatg attcctttac atcagcttct gcaagttgaa
     6781 aagcaatgaa ttgttctttc aatgctttta aattctctgt aactgcctca tctattccga
     6841 atgcttgagc ctgtttgatt atttttagcg tattaataat tccattacct aaagtcagta
     6901 aggtttggat tttaacccag gcagagtctt tatgttcctt gcaatagagt tgacatggtc
     6961 cttcaaaagc ggcaaaacga gataagttga aagtattagg gtctactgta aataggcggc
     7021 gcaatacaat gctttctttg tagtaaggaa caaaggcatg tccgtgaatc gcttcaccac
     7081 cactattatc gtagagagca aacagattag gggtgctgtc tattctttct tttgcccaga
     7141 cattattggc ttcacacgtg atagctagtt catcaacgta aagattaaat tgtttgacta
     7201 attccagtag gttgattgga ttactatcaa acgaaaattt ttccatgtct gaagttttta
     7261 agatgtttgt ccattgattt atggtttctg aaaaagaggt tgaagaggtc ataggaagcg
     7321 tcatgtattt taattccaaa atactttgat acagttcttc aggattgctc aatggggaca
     7381 tatctttaag aacagcaatt tttcctaaac gattttctaa taatgcggcc atggcaatcg
     7441 caaaagattt gcaattcccc tccgggccaa atgtcgcctc ttttaatgaa tccatacaca
     7501 tgtaggcagc aagttggatt ttggtttttt cttctatcag atcggttgga atgtttttga
     7561 gtgcaccagt tactatgtca ataagcaatt caggatttag cttggattgt aattgccggg
     7621 ctttttcagc cattgcaggg aagagcccat tgatcttctt atagttcagg aaataatctt
     7681 tagttagtga tttataatta aaccaacctc tgttttcata agcataaagg agattgtcat
     7741 tattgtttgg atgagcgaaa taacggaagg catctcccca atcgattcgg ccaaattgtt
     7801 tgcttttttc ttctccttct cctttgagta caacgatatt gccactatgt acactatggg
     7861 ctcccagcaa tagagaaaac ataagggcca tagaaaggcc aaaatattta ttttgttttg
     7921 tgatcccggc ataatagcct ggcccaaaga aggtttcctt tatcgtatta cgatcactgc
     7981 catcaccgta gctcgttcca atgattttgt gtaattcatc aaatgaaacc agtggctgta
     8041 tcagtccata ggacttgtct tcaaattgaa tgacatcggc gcaaatcaat gatggaaaat
     8101 agctttcatc aatcaatcct cgcttcatga actcttttaa tagcaagcct gcaaataatt
     8161 ccgtgaatag ctcacgaggg tcgcccggtt tttttataaa aaattttcgt ccatcactgt
     8221 cttcgtaaaa accatcgaca tcacttttat ttttaccacc tgttttttct ttaaatcgaa
     8281 ctatttcttt accttgataa gttaacataa tgactctgac ctaaaacaac actttgtgcc
     8341 attataacca ccaagtatta actaaacctt aagaaaatct taagaattca tctgcaaatt
     8401 tctttctgct ttggccggta ttgctcactg attcttgctt taacagttgt ttttttacgc
     8461 ttcattagac aataaagacc aacagagctt aaatagggga acccaaagag aaatactatg
     8521 tgttttatcg tcccagccag agcgatatca gaaactaatc ccattacaaa aataaattga
     8581 aaggaacgat tttttataaa atctgtaata aaaggttgga gcatttttaa tccattaact
     8641 caaatgataa ggacgagaat tagttacata cccatttgtt ctcagcaaaa atggaataca
     8701 cttcattatc tccaatgaca atgtgatcca gtaacctggc gtctaccagg tccagagcat
     8761 ctcgaatgcg ttctgtaatg gcaaaatctt gctggcttgc atcagacaag cccgagggat
     8821 gattatgtgc cagaattaaa gcagcagcat ttaattgcaa tacacgttct acaatcggtc
     8881 tgggatagac tgttgctgtg ttgatggttc ctgagaataa ttcttcataa gcaatgattc
     8941 ggtgttggct atccagaaag agtgcggcaa aagtttcatt tttgtagtct cgcagtcgtt
     9001 tttttaaaaa agcataagtt tgttgcgtgt tagataactg gatttctttt tgcagatcaa
     9061 tgaaatcact acgatgacat atttctttcg cagcctgaag ttgcgcatag cgaacttctc
     9121 ctaatccatg gattgattta aagctttgca gatcagcatt gaggatatta cgtaaatttc
     9181 ccaagtgttt ggtgagctca taggctagtt gcaaacaaga ttttttattg ttacccgaac
     9241 tgatgaaaac ggctaacagt tcgatatcag agaggctatg tactccatta gtaagtagtt
     9301 tttcgcgcaa gtcaagttgc tgcgctgtat gggcaaccat cattttgtat tccttttata
     9361 ttcctttatt gcgaagtttg tgctttgatc ggggttattc ttatcaaaaa tggcgagcca
     9421 tgcaagattt tattaataaa aaaattctac ttggtgtttg tggtggtatt gctgcttata
     9481 aatctgctca tctggttaga gaattgactc gtttgggagc cgatgtgagg gttgtcatga
     9541 ctcattcagc acaacagttc attaaccctt tattgatgca ggcattatca ggcaatgaag
     9601 tgaggattga tttattcgat actcaagctg agcgggctat gggacatatt gaattagcca
     9661 gatgggcgga ttatttgctc attgctcctg cgtccgcaaa ttgtcttgct aaaatggctc
     9721 aaggtcttgc tgatgatttg ctttcaacat tatatctcgt tactgaaacg ccagttattg
     9781 tttgtcctgc catgaacaga agcatgtggg cacatccagc aaccaaggca aattgtgaat
     9841 tgttaaaaga acgaggtgtg ttttttgtcg ggcctgaaga aggctcccag gcctgtgggg
     9901 aacatggatt gggaagagtc agtgaggcgg atcaaattgt gaataccttg cgcttactgg
     9961 atgtgaatca actattgcat ggcaaacgaa ttgtgattac tgcaggtcct actcgcgagt
    10021 cgattgatcc agttcgttat ttaagcaatt acagttcagg aaaaatgggt tttgcaatgg
    10081 cagaagccgc cgttatggca ggggcacaag tgacattaat tagtggccct tgtcatttac
    10141 aaacattttc ggacatcgag ttaataagag ttgaatcggc gcaatcaatg ttcgacgaag
    10201 tgaacaggca tttacaacaa ggagatttgt ttattgggac agcagcggta tcagattata
    10261 gcgtgctgaa tcctgcttcg gaaaaaatga aaaaaaatga gaattctgaa ctcactcttc
    10321 agcttgttag aaataccgat attctttctg aagtggctaa aagtggtaaa gcttcctttg
    10381 ttgttgggtt tgcagctgaa acaactgatg tgatttccta tgcaaaagaa aaaatgaaaa
    10441 ataaaaaact ggatatgatt atcgccaatc aggtaggtaa gggaatggga ttcgaaagtg
    10501 atgtaaatca agtaacagtg attacaaaaa acaaacaaat agagctgcca ttaacccata
    10561 aaacacgcct ggcaggccaa attattgcaa tccttgctgc aagtctgcaa aattaggcgc
    10621 aataaaacga ttgagaatta ttatgcatca agttattcag ttaaaaattc tggattcaag
    10681 gataggagat accattccat tgcctgccta tgccactgat gggtccgccg gtctggattt
    10741 aagggtctgt atctcggagc ctatgcaagt agcacctcag caagcagtat tgctgcccac
    10801 cggcattgct atttatattg ccgatccaaa gctggcggca gtgattttac caagatcagg
    10861 tttaggccat aaaaatggta ttgttttagg aaaccttgta gggctgattg actcggatta
    10921 tcaaggagaa ttaaaaatct cttgttggaa caggagccag gaacatttta ctgttaatcc
    10981 tggtgataga attgctcaat tagtttttat cccggttgta caggcttctt ttgaggtggt
    11041 caatgaattt acagaaagct ccagaggaga aggtggtttt ggcagctccg ggagatatta
    11101 aatgacctat caacaaaagc aaattccccg ctctgtcttt cgtgcctatg acattagggg
    11161 gattatcggt aaagaactgg atgagaattc tttttatagc attggcttag ctattgcacg
    11221 ttatttacaa aaattaaatc gtcgacaaat atttgtagct cgtgatggac gtctgaccag
    11281 ttatgcattg gctaaggcct tgaaagaggg tttgctagac agcgggatag atattcttga
    11341 tcttggtgct gtggcgacac ctgtcatgta ttatgctacc catacgcaag ggattgattc
    11401 tggattgatg gttaccggaa gtcataatcc cgctgattat aatggtatca agttagtatt
    11461 ggctggaaaa acattaattc aagatgaaat tgatacactt tatgctttgc ttggtgaagt
    11521 caagcagtca gctgttcgtg gcaaagaaac ggcttatgct gtgatagaag actatattca
    11581 gcgtatcgtg agcgatattc aattgaagcg ccctttaaaa gtagttgttg attgtggcaa
    11641 tggtgtggcg ggcccagtta ttcccagggc tatttcgaaa ttgggctgtg aagtcattcc
    11701 cctgtattgt gaagtggatg gtcgatttcc caatcatcat cctgaccctt caatagaagc
    11761 taatttagtt gatttaaaag cggctgtggc aaatcatcaa gctgatattg gtttgggctt
    11821 tgatggggat gctgacagac ttggcctagt gacaaataaa ggcgaagtga tttggcccga
    11881 tcgtttaatg atgttttact cacgagagat tttaagtcgt aatccaggtg ctactgttgt
    11941 gtttgatgtg aagtgctcca gtcatttgga gaaagaaatc caggcagcag gcggtgttgc
    12001 cagaatgtgt cccactggcc attcaattgt gaaagcggtg atgaaaaaag aacaagccat
    12061 cctggctggc gaaatgagtg ggcatctgtt ttttaaagat cgctggtatg gatttgacga
    12121 tgctttatat agcgcatgcc gattgttgga gattatcagt tcttcccatt tgactgtcag
    12181 tgaacaattt gagttgatcc ctaatagtgt caatactcct gaaataaaaa tagcgattac
    12241 tgatgaagaa aaatttgatt ttattcagcg ttttagtgag caggctgatt tccctgatgg
    12301 caggattttg aatattgatg gtttgcgtgt tgaatttcca aatggatggg gattgttacg
    12361 agcatcaaac acaactcctt gcctggtcgc acgttttgag gcgacagata aagatcatct
    12421 ggagcaaatt caatatttgt ttaagaaaca aattcagcgc ctggattctg agttggattt
    12481 gccgttttag tgtttaggaa tatgaaaaat tcaatatagt gctattctgc tcagaatttg
    12541 tcagatttgg acaggactaa cgaaaaagcc caaggttcat tttttaatat agagattagg
    12601 gggtttcgta agttctgttg gaaacaaaat gagatgttga cttcttaaca caatacaaca
    12661 tgacttccag cacttgtttg ttttgtgttc ggcctggtat tttctcttgt ttgataagat
    12721 gaaattggta tttattgatt aagctctcta tgtattcggg ttgatggcta aatcgagcgc
    12781 tgctttccag tttccacgga gttgttgtgc tgatttcggt agtgaaaata aaataccctt
    12841 cttgcttcag atgttgattg atggaattaa aaatatcatc gagattacca aaatagggta
    12901 ggacgtctgc agcgaccgct aaatcgtaat cgtttttatc ttttcttaaa aaatcgatta
    12961 attccgagca taccagaaga tcatagatgt ttttttcctt tgctcgagct atcatttttt
    13021 ctgctatatc tactccggtt aagtgtttgc ttatttcacg aagcacaata cctgttaatc
    13081 cagtaccaca acccaagtcc aaagaatgac tggtttgcaa aagttgaagt tggtggatga
    13141 ggcggccaat gtgatgcgga atcttatagt gtaattcacc ttgcatgtgt tgatcgtaat
    13201 acaaggcata attattaaat aaattttggg catatttttc tgtagtatta acgctcgttg
    13261 ctcctgtaat ggcattcagc atatgcctgc tgaccacatc atcggggttg attaccagtg
    13321 cgcgctcaag atattcgcgc gcggtttctc gcatgtccat ttttaaataa atagcggcca
    13381 gattattcaa ggatggggtg tgatcacttt gtaatgttaa aatctgatca aacaaaaggg
    13441 tggcttcatt gagatggccc aatgccattt gagcgactcc ggaattgtat aaatactcca
    13501 gattatcggg ttcttttttc agtaaaacat cgtaatgcat gagcgcattt tcaaatcgat
    13561 catgatgcat gaaggtggct gccaaattat tacgtgcatc aatgtcttca ttatccaaag
    13621 ccaaggcttt ggtaaaataa tcaacagcca attggttttg ttctcttttc aaagcgatca
    13681 cgcccaagtt aattagggat tgaacatgtt catgatcttg ctctaacact ttatgaaatg
    13741 cctgttctgc ttctgagagt aaattgtctt ccaaatgcaa aatacctaag taaaattgag
    13801 cttctctgtg ctggggattt agagctatca cattattaaa ttgtgttttg gcagcagaca
    13861 attgttgatt tttgagaagt aacaaaccta aattaaagtg agccgcgcta aagtcaggtt
    13921 ctgtattcac tgcaatcaca taatgatgca acgctttctg gtaattgttg agtaatgcgt
    13981 aggttgctgc cagattatta tgagcctgga catactctgg tttaatctca atggcttgct
    14041 ggtagtattt aattgcctca tctaattgac ctgccttttt ataagcgtta gcgagattat
    14101 tgagtatacc aacgtcattg gggttaattt ttctggcttg caaaaaatat aaaatggcat
    14161 tttccatatc acccaattgg gcataggtta atcccaggaa atgcagagtt tccgtatgtt
    14221 tgggtgattg ggcaagtatt tgctcatata agctaatggc ttgaggtaaa tggccttcat
    14281 attgaagctt ataggcttgt gcaaaaagag tatctacttc actgttcata ttaatctgga
    14341 agaagggcgc gtaattggtt taaagctttt gcgcgatggc taatcctatt tttaatttta
    14401 gcaggtaatt cagcggctgt gcattgatat tcgttgagat aaaataccgg gtcataccca
    14461 aagccatttg ttcctgaagg tttcatgcta ataacgccat gaaatacgcc tgtggcaatg
    14521 agcggtgttg gatctttggc atgttgcatt agcgctattg cgcaaaaaaa ataggcttgt
    14581 ctttgttctt gggataaatc agccatttta cttagcaatt gttgaatatt gtcttcgtca
    14641 tttgccttta ttccagcata gcgtgctgaa tagatacccg gctctccatt taaagagggg
    14701 actacaagcc ccgagtcgtc ggctaaagct ggtttattgg ctaatgagct ggcatgacgg
    14761 gctttgagaa tggcattctc tataaaactt aatcctgttt cttctgcatc ggatataccc
    14821 aagtcggctt ggggaatgca tatggtgggg gctagtaatt gttctaattc ttttattttt
    14881 ccagggttac tggttgccaa aataatttct ttcattctta ttccatgtta ttaattcagt
    14941 attcataaaa aggataatta tgcatgagaa ttttggaaaa aaatatcaag aagactccct
    15001 ggtatttaaa accattcttc tggaatcaaa aaagaaaata cggccaaata ctcgaacccg
    15061 ctttgatttg ggcaagaatt cccaggttat ttttggcagt ttctgctatg tatggcgtga
    15121 tagacagaaa aaaatcacca ctttccccaa tattacgctc tctcatcact gtcagggttt
    15181 ctcaaattaa ctggtgtgag ttttgtgttg atattaattc agcaacttta attaaaaggg
    15241 ctggaagtga agcacattat aaacaactgg cgaaatggag gagttcctca ttattttcac
    15301 cagaagaaaa agcggcattg gattatgctg aaaaagtaac ttattctgat caacgtcctg
    15361 atgatgagtg tttcaatcaa ttgaaattgt attattctga acaggaaatc attgaattga
    15421 cagcactgat tgcatttcag aatatgtcca gtaaatttaa tagtgccctt ggcgttgaag
    15481 cacaaggatt ttgttcgagg caactgactg gcaaaagctg aatagtcctg ccagtcagta
    15541 cttgcaatta catagagatg cttttaccta ttgtattatc gttgaatcga tcatgcctta
    15601 gttgttgttc atccgtaata agatcaacta ccgtgttaaa atcctgagcc tcatcttcag
    15661 taatctctat tgaatcggct gcttgtaagt aatgtaattg acttaaatcc tgttgaatgt
    15721 ctttaatacc tttttgggga tgtttttcta tgtaagcatt cgatctggca atatagtcat
    15781 tgacaaaacc ttgatatacc gattgcccat ccaacatttc cagctcacac aaatcagcaa
    15841 agtgagcatt gacccttcca aatttatcga ctccaaattt ggaagcaagg cgttctttag
    15901 cggtttcctt gactcgaaca taatgttttc tatccagctc atcacgatct tttcgatact
    15961 cttccggaca ggtatccgta atgggagccc ttttttgaga tttggtttgc ccagtttgta
    16021 tttcatgttg atatttgctg actttacttg ataatgtcga agattctaat gtttgatctc
    16081 ccaagtgttg aatatgacat tggtaaggat cagcatgttt atattttttt cctggagtaa
    16141 catgccagcc ggtaaattct aacaatcttt taacaatgct ttttaatatg ttaggtagcc
    16201 atggtgattg ggtaatcata tcgcaaacgg ctgctttaaa tgacttgaaa acattgttca
    16261 tgatcgcacg aactttgatg tccgcttgct cttctaattg cctttttacc tctaatgcaa
    16321 tgctagctcc gtagcaatcc ccctgtaaaa tgatgtcgtc cgggtgcacc ccttgtttaa
    16381 ttaaagagga aaccacagaa agaccagcat tgattaagtc attcgcctct ctcacctttc
    16441 ctgaactgag gcctgtccct gggaaattaa aagcgtgtac agttgctcca gtttctttgc
    16501 aagccgtgct gatatcgcga aaacaggctt ggtagtaagt attcgctccg ggaaaataga
    16561 caatatgttt cccagtgcca ggctttttag gctcatttga ttctggttgg catttcatga
    16621 cttctaactg acatgattca ccagaaacca gctttgtttt cactttcttc agacttaaat
    16681 ccaggccttg aaactcttta taatagtgtg attttttatc ggttttttgt ttttcaacga
    16741 aatctctaaa aggagcttcg tcatcaactt cgcccttttt tccgtagcct ttgtttttat
    16801 accaatcacc ggcccctgct ggaaaaaggg aattagctac aaattgttgt aa
//
LOCUS       DATNSN010000062        16838 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.062, whole genome shotgun sequence.
ACCESSION   DATNSN010000062 DATNSN010000000
VERSION     DATNSN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16838
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.062"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(445..1329)
                     /locus_tag="VAH76_001634"
     CDS             complement(445..1329)
                     /locus_tag="VAH76_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEO1386262.1"
                     /translation="MISDHFICKKAKIIAMSSLFITTVAFAAPSIQYASPMGEENWRM
                     TGNPLRCGLSLTIPNYGIGYFEQYATQSPHFILRKWDQVQRALPAQVVASPPVWKPLL
                     KGSYLVTRTYIKPGEYGLFLNRQPALKLLTFLSQGYQANFSYLSEEGFRTTVTLSPVR
                     FQKVYSQYQRCLGGLLPFNYEDVKESVFHFGVDSKLLSDADKAQLRRIAQYVDADVQI
                     DVIKVIGHADDSGRKGYNNAISQYRAESVKNYLIRLGVPQRKLSITWVGALQPIARND
                     TDEGRAANRRVVVTLIKK"
     gene            1399..2436
                     /gene="pyrC"
                     /locus_tag="VAH76_001635"
     CDS             1399..2436
                     /gene="pyrC"
                     /locus_tag="VAH76_001635"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO1386263.1"
                     /translation="MQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKP
                     ALTNLATIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYP
                     AGATTNSEEGAKSLKALYPLFETLQTSNLVLQIHGEVTHSDIFEREALFIDEYLKPIA
                     RHFPKLRIVLEHISTKAAVDYVLEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLP
                     ILKHEKDQKALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPFAVALYTQI
                     FDELNQLKKLNDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMPFGSTHVVPIAAGD
                     TINWSINGFSQ"
     gene            2420..3043
                     /gene="rnt"
                     /locus_tag="VAH76_001636"
     CDS             2420..3043
                     /gene="rnt"
                     /locus_tag="VAH76_001636"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEO1386264.1"
                     /translation="MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIV
                     LLDMDEQGLLKKESSYFEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQIALERLF
                     KPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
                     GETVLAKAVQTAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI"
     gene            complement(3169..3774)
                     /locus_tag="VAH76_001637"
     CDS             complement(3169..3774)
                     /locus_tag="VAH76_001637"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin C"
                     /protein_id="HEO1386265.1"
                     /translation="MSVLVGRKAPDFTVAAVMGNGEIVDKFNLHEHLKGKYGLVFFYP
                     LDFTFVCPSELIALDHRIEEFKRRNVEVVAVSIDSHFTHNAYRNTPVKNGGIGPVRFT
                     LAADMTHSICQSYGVEHPVAGVAFRGAFVIDTNGMVRSQIVNDLPIGRNIDEILRIID
                     AVQFFEENGEVCPAGWQKGQAGMKASPKGVAEYLSEHSESL"
     gene            complement(3827..4147)
                     /gene="grxD"
                     /locus_tag="VAH76_001638"
     CDS             complement(3827..4147)
                     /gene="grxD"
                     /locus_tag="VAH76_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEO1386266.1"
                     /translation="MVDTLEKIKKQIAENAIMLYMKGTPKMPQCGFSARAVQCIEACG
                     VDFAYVDILANPDIRQVLPQFSDWPTFPQLYVKGELIGGSDIIAEMFQQGELEPMLRD
                     AVAA"
     gene            4475..5053
                     /gene="sodB"
                     /locus_tag="VAH76_001639"
     CDS             4475..5053
                     /gene="sodB"
                     /locus_tag="VAH76_001639"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEO1386267.1"
                     /translation="MTFTLPQLPYALDALAPHVSKETLEYHYGKHHNTYVTNLNKLIP
                     GTEFESMTLEEIIMKAKGGIFNNAAQVWNHTFYWHSMSPNGGGEPKGRLAEAINKSFG
                     SFAAFKEQFSQTAATTFGSGWAWLVQDQSGALKIISTSNAGTPMTEGLNALLTCDVWE
                     HAYYIDYRNRRPDYIEAFWSLVNWDFASSNLK"
     gene            5113..6282
                     /locus_tag="VAH76_001640"
     CDS             5113..6282
                     /locus_tag="VAH76_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEO1386268.1"
                     /translation="MSLITSYSPMPVTFTHGEGVWLYDEQGKKYLDGLSGIAVCGLGH
                     AHPDVTRTIQEQAARLIHTSNVFHIKEQELLAAKLTSMARMEQVFFANSGAEANEAAI
                     KLTRLFGHKKGIETPSIIVMEKAFHGRTMATLTASGSRKVQAGFEPLVPGFIRAPFND
                     LDAIHTIAANREDVVAVMVEPIQGEGGIYPAEEGYLRALANLCEQNDWMLILDEIQTG
                     NGRTGKLYSCMHYDIQPDILTTAKGLGNGMPIGACLMSKRAKDLFKPGNHGSTFGGNP
                     LACATALTVLEVIERDKLCERVTKNSALLMDKLISNLGEHPHVKAIRGKGYMIGIELD
                     RPANDMRVLGLENQVLFNITAETVVRLLPPLIISEDEIDELVNRITRTINQFTNQ"
     gene            complement(6411..7178)
                     /locus_tag="VAH76_001641"
     CDS             complement(6411..7178)
                     /locus_tag="VAH76_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1386269.1"
                     /translation="MTLKITFLGSGSSFAIGPENYQSNVLLELENDTLLIDAGTDIKY
                     SLRDQGLSYKDIKNVYISHLHYDHCGGLEWFALITYFDPNHSHKPCLIASDKIIKELW
                     DKSLSGGLSTLPHERANLSAFFDVRPVKQYEGFVWQSIKFKLVQTVHYYSEYELMPSF
                     GLIFSYNKSRILFTSDTQSCPVQLISFYEESDIIFHDCETTESKSGVHAHYSELVKLP
                     SHLKKKMWLYHYNPGKLPNAKKDGFLGFVAKGQSFVF"
     gene            complement(7334..8116)
                     /locus_tag="VAH76_001642"
     CDS             complement(7334..8116)
                     /locus_tag="VAH76_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO1386270.1"
                     /translation="MLLKCIEKLIDGYFAVIPRHAPEFENINGALVIAHRGAHNNARG
                     IIENTLEAFQNAKEAGCWGVELDVHLTKDKVFIVNHDPTLNRLWNKDIAISDLSFSEL
                     RALECRIPSLSEVVRQFGNSMHLFIELKTAVEEEEILVQTLQHLTPCKDFHLLTLQES
                     TFKKLCSFPRQSLLLVAVHNNVKKFCELSIKEGYGGVLGNYLLLTNRVLKYLQDANQH
                     FGVGFVDSKNSLYRELNRGVKWLFTNEAITINQYLNDLKNRM"
     gene            8368..10038
                     /locus_tag="VAH76_001643"
     CDS             8368..10038
                     /locus_tag="VAH76_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEO1386271.1"
                     /translation="MAIKVTKRGMDLLRDPVLNKGTAFSLQERDEFALHGLIPTTVET
                     LEQQVIRCLDAYSAKENPLEKHIYLRALQDRNEVLFYRFIIDNLVDILPIIYTPVVGQ
                     ACEMFSHIYRQPRGVFLSYPERDKLDSIIQNIASTRSIKVIVVTDGERILGLGDQGAG
                     GLGIPIGKLSLYTSCGGIHPSNTLPIILDVGTNNKERLGDPEYIGWRHARISGKEYDD
                     FVDQFVQSIKRHMPHVLLQFEDFAQQHAYPLLERYKNQLCTFNDDIQGTASVAVAAIL
                     AATRVTNTPLKEHRIALLGAGSAGCGISEQLVHAMMNQGLSEEEARSRFYLVDRYGLL
                     HDEMTDLLPFQKGFVRSSTSLQNWKLAKKGEITLTDVINNAQPTILLGVSGQPNQFKE
                     AMIKTMLSYCERPIIFPLSNPTSRAEAIPQDLLNWTAGKALIATGSPFEPVVINGHKI
                     EIAQCNNSYIFPGVGLGVVAGQAKRVTDLMMMAAAVALSELAPAIRTGEGRLLPELNS
                     IREVSQHIARAVILQGIKEGHIGPMNNNEIDDSIKRTMWTPQYEPYVL"
     gene            10268..11236
                     /locus_tag="VAH76_001644"
     CDS             10268..11236
                     /locus_tag="VAH76_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO1386272.1"
                     /translation="MSIITTNLNRAIHDLQQGKPVAIPTETVYGLAAPINNNAAIRAV
                     FEMKSRPLNHPLIVHIASNWDLHDLVEEVPEYAHQLAGKFWPGPLTLVFHCKKDQINP
                     LITGGQSTIAIRCPDHPLTQKLLQKLNIPVVAPSANPFGKISPTTALHVKQSFNDAEL
                     TILDGGRCRVGIESTIVDATNPDSYQILRHGIIDEKTIAETIAIGCSTQKNDIRVPGK
                     LDSHYQPQKTLYYFDQYETLSEFCQKRKGTVYVIANRQPPGVETNLFHPLGMHPEKSA
                     YDLYYQLRLADTSDAICIAIELPPNTSEWQGVRERILKAGFPYSQQ"
     gene            11319..11405
                     /locus_tag="VAH76_001645"
     tRNA            11319..11405
                     /locus_tag="VAH76_001645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11353..11355,aa:Leu,seq:cag)
     gene            complement(11410..12261)
                     /gene="psd"
                     /locus_tag="VAH76_001646"
     CDS             complement(11410..12261)
                     /gene="psd"
                     /locus_tag="VAH76_001646"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842959.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEO1386273.1"
                     /translation="MFRDVLKTLPQYLIPKHGITALAGYFADVKSPRLKNFLIRNFIR
                     KFDVDMSEALIEDPKSYDCFNDFFIRHLKPECRPLSQSDVICPVDGCISEIGKIERGQ
                     LLQAKGKYYSVQELLACDGQLAEQFVQGQFATLYLSPKDYHRVHMPIDAELVSMTYIP
                     GALFSVQPATTRVVPKLFARNERLAIFFKTKIGPMVMVMVGATIVGAIGTSWHGDVKR
                     AKKLERFDYSEQFPDKIISQGSEMGYFKLGSTVVLLFANGEKIQWDKELLAGSKIQLG
                     KPMAIIT"
     gene            complement(12354..14969)
                     /locus_tag="VAH76_001647"
     CDS             complement(12354..14969)
                     /locus_tag="VAH76_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2975 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386274.1"
                     /translation="MGLPKKALKESQLQFLTAGTAVSDSSHQTYKVSFIENGVIKNAF
                     YKKLDPKNHYPELLAKISVAVSLFKRIFQGRRSAEERLVFDDEERLVGTLSISVDGFK
                     GFNFHKESVPQESSAKEQVIPSTRTLIEKSFMEILLGRWFLDDDDGHPHNLSLAGDID
                     FDMFFYWFTIYMKEPRPAIGIPKTRVNLTVRDWEGFPNVKDSKPFHWPTYKNPGQETL
                     PTVLPVQDKLVNLILEKTYPDPGQFEQLAHEPVAQEQKFAAALKILLTYQPEMIRKRL
                     TELFGEMTLNYTSLDETDVALRSQYEKTFPHLCNESTNIKPFVDFIMNLYQMHYDNLY
                     RVVVFYMGCENNGYGVPLPATNSALYHKPSFYKDIVEWARTQNITIFSKDDSSIKFDE
                     DELRRRYHQVWRDAYAPTFRDLLHDSYSLTNKLLQQVSTFHVVLNEVEGKKPTDDTLT
                     NAWELFGTMPELSLEKITPLISVDKDSKLRTALILLVEFTTQFHAVAKTYYQKDRKDL
                     TEEDNLEFSEQLVQLYTNYNLKIRQSLAHTSTLAGEFNRIAVGLKQYTERANFQLHLT
                     TTDEQMKEATVATTPKEILPHTHEDVIRQFNDSLFLWAKNLRPEDLSHHISEIIDKYY
                     APTIELLSKRHRAQPVKEYLQASVNESGENRLAYILSAGEGDTGALNTLLIQHLTPYM
                     LQTYPLLSIRNAVKEGNFDKDLEIFTKAAVDFAKHDRRFIHLYNVEGKSLFFKTMYEW
                     IDELPATKFKGLLESALKDYEGKLWWSTSRRSEVEGYCSKFSQAKIVAMTFLNGKDSS
                     SLNDVLFDKIIAAIQKDINKNKEKLKIPGFRLINCYNAKEHRADYFKEVKNYAEPISH
                     RQETTLNSNVTSLVV"
     gene            complement(15232..16761)
                     /locus_tag="VAH76_001648"
     CDS             complement(15232..16761)
                     /locus_tag="VAH76_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386275.1"
                     /translation="MSITAQELVKQYKLRLTPAMENDLLSEESRLKKELEAVPFNSEE
                     TLYKSILQMIIVFYEENTLEENRSLLQDHELIKQLSALMWDDIQIKLIPFLIQKNFTL
                     SEIKELLFDEAYYRSLHVLVDFGLTQDIPELLAHQEKREQLKFINTLANDHCRKLCLI
                     FWVKGSLSIKEIQDIVNATSHYPMLAETLIALDKTKTISIKQLKKLALDPKKHQQESI
                     LYHYSEQFKAYNLRKSDLSQLNLDDLDALGKSFKVLKEAGIANDYAYRLVLKNNKTGQ
                     LLRLFLPGLAKIESLSHRKALIELLYIGAQKGVVTQGKALLQIKDSNLLVLARALRER
                     FICVQQMQDLGFKKEIIAFTGEENNINSSRFRHVIMRVEEKCKDIHERLRKSSLDKDK
                     VGNWQRADEKYRQTLYSIAYDGITKSGVDLHIKMKSAEKEILSIVDPEIKSIIHKVLV
                     VIANIIITALTLGFANDLKESATGNYWFFNQSPSGEVIRALNKEVLTTIDSPELITIS
                     P"
BASE COUNT         5232 a         3433 c         3042 g         5131 t
ORIGIN      
        1 ccaatatccc atgcagttct ttcgcaagga acagttacat tacctggggt gcaaactggt
       61 cccatggttc cagcaaacac tgcaccgctt cctaaggcga gtactgccac tgctgttttt
      121 ttcaaactaa acatgccttg tctccacttg tttattattg agtttccatt ttattgataa
      181 ctcaccattt ggagttgtca atagcacgca tcgtcgctta ggttcgaagc atgcaacgat
      241 atgagtatct gcagtgattt taaataagtc aagaaaattt attaaaaatt ccaatatttt
      301 catgacgata taactaaatt tgaacagtat ctctattttt ttaacattat tcccatgtct
      361 ctttcaaagg aaatttgctg accaagaagg ggattttcga ggatgaaatg ccatcataaa
      421 aagatgcttt tttaaccaca aaatttattt ttttatcaat gtcacaacaa cacgtctgtt
      481 agctgctctc ccctcgtcag tatcattcct ggctatcggt tgcagagctc ctacccatgt
      541 tattgagagt tttctttgag gtactccaag tctaatcaaa taatttttca cagactctgc
      601 tcgatattgt gaaatggcat tattgtagcc tttacgtccg ctatcgtcag catgaccaat
      661 aaccttaatg acatcaattt gtacatcagc atccacgtat tgggcaattc gtcgtaattg
      721 cgctttatca gcatcagaca ataacttact atccacccca aaatgaaaca cgctttcttt
      781 tacatcctca tagttgaagg gcaacaagcc accaagacaa cgttgatatt gcgagtacac
      841 tttttgaaac cgaacaggtg agagtgtaac cgtagtgcga aatccttcct ctgataaata
      901 gctaaaattt gcctgatatc cttgcgataa aaatgtcaat aattttaaag cgggctgtcg
      961 atttaaaaat aatccatact ccccgggttt aatataagtg cgagttacca gataggaccc
     1021 tttcaataat ggcttccaca ctggaggact tgctacaact tgagcaggta aggcccgttg
     1081 aacttgatcc catttacgca gaatgaagtg aggggattga gtagcgtatt gttcaaagta
     1141 gccaatacca taatttggaa tcgtcaatga caaaccacat cgaagaggat tgcctgtcat
     1201 acgccaattt tcttctccca tagggctggc atattggata gagggagcag caaaggcgac
     1261 agtagtaatg aataaagaac tcatcgcaat aatttttgct ttcttgcata taaaatgatc
     1321 tgaaatcatt ttttgctcat cccaattgcc taattcatgt tattctcttt tatcggcaat
     1381 aatgcaggaa cctttattat gcaaactctg ataattaatc gccctgacga ttggcatctc
     1441 catctccggg atggagagtt tctaactcat accgtccagg cgtctgccca acattttgcc
     1501 agagcgttag tgatgcccaa tctcaaacca gcactaacta atttagccac aattgagagt
     1561 tatagaaaca gaatcatttc ggccattccc aaacaaaact cctttattcc ttatatgact
     1621 ttttatttaa atgagtccgt taaaccagaa gaattacaac aagccgcctc tctttcttat
     1681 atagttggag caaaacttta tccagctgga gcgacgacaa attctgaaga gggtgctaaa
     1741 tctttgaaag cactctatcc tctttttgaa acattgcaaa cgagtaattt agtcttgcaa
     1801 attcatggcg aagtcactca tagtgatatt ttcgaacgcg aagcactatt tattgatgaa
     1861 tacctgaaac ccattgccag acatttcccc aaattaagaa tcgttctgga gcacatatca
     1921 accaaagctg ccgtggatta tgttttagaa ggacctgcta accttacagc aacaataact
     1981 cctcaccatt tgctttataa tcgcaataag ctactcgcag ggggcattag acctcattat
     2041 tattgtttgc ctattttaaa acacgaaaaa gaccaaaagg ctttacaaat tgcagcgacg
     2101 agcggaaatc caaaattttt tgccggcact gacagtgccc ctcatgccat caatgccaag
     2161 gaaaatgcat gtggatgcgc cggtatttat tcagctccat ttgcggtagc gctctacacg
     2221 cagatctttg atgaacttaa ccaattaaaa aaacttaatg attttcttag cagatttggc
     2281 gcagagtttt accaactccc tattaatcaa cagcaattgg aactcattaa atcacctcaa
     2341 actatcccca actcaatgcc atttggatca actcatgttg tgcctattgc tgctggagac
     2401 actataaact ggagtattaa tggtttctcg caataatatt ctaagtaaca ccattaaaaa
     2461 tcgctttcgt ggctttttac ctgttgttgt tgatgtagaa actgcgggca tagaccctca
     2521 aaaaaatgct ctcctggaaa tgtgtattgt tttattggat atggatgaac aaggcttgtt
     2581 aaaaaaggag agtagttatt ttgaacatat tttacctttc ccaggtgcag aactggatca
     2641 aaaatcactg gaatttaacc aaattgaccc ttaccagcct cttcgttttg cagtagatga
     2701 acaaattgcc cttgagcgat tattcaaacc catccatgac ttgctaaaaa aaacccgttg
     2761 ccaaagggct gttttagtag ggcataatgc ctggtttgat ttgttattca tcaaagcggc
     2821 aatcaaaaga accgggataa aatcgccatt ccatgccttc acttgctttg atacagcaac
     2881 attagctggt ttgatttttg gagaaaccgt attggccaaa gcggtgcaaa cagccgggtt
     2941 acattttgac agccaggaag cgcactctgc catttatgat gcagaaaaaa cggctgattt
     3001 gttttgcctt atgattaaca agtggcgaat gctaaacatc tgatatcaga aaaacataaa
     3061 tagcctggat tacctaaaac tgtaatccag gagtttgata gaaattttct tgtgttatgc
     3121 ttctcaataa aacacaacaa caaaaagacc ccatttcctt gtcttatatt acaaggactc
     3181 ggagtgctca gacaggtatt ctgcaacacc tttaggagac gctttcatac cagcttgtcc
     3241 tttttgccag ccagcaggac aaacctcacc attttcttca aagaattgaa ctgcatctat
     3301 gatacgaaga atctcatcaa tatttcttcc tatcggcaag tcattgacga tttgcgaacg
     3361 aaccataccg ttcgtatcga taacaaaagc gcctcggaaa gccacaccag caactggatg
     3421 ttctacacca taggattggc aaatactatg agtcatgtca gctgccaaag taaatctcac
     3481 tggccctatt ccaccatttt tcacaggagt attcctatac gcattatggg taaaatgtga
     3541 atcaatagat acagcaacca cttcaacatt acgtcttttg aactcttcga tacgatgatc
     3601 aagagcaatt aactcggaag gacatacaaa agtaaaatcc aggggataaa agaaaactaa
     3661 tccgtatttg ccttttaaat gttcgtgtaa attgaattta tcaacaattt ccccattgcc
     3721 cattactgca gccacagtaa aatcaggtgc tttacgccca actaagacac tcattctatt
     3781 atctccatta gcggtgggtg agtaaatccc acaattgtta ttaaaattat gccgctactg
     3841 catcacgcaa cataggctct aactctcctt gctgaaacat ttcggctatg atgtcagatc
     3901 ctccaattaa ttcccctttc acataaagtt gaggaaaggt aggccagtca gaaaattggg
     3961 gcaaaacttg acgaatatca ggattggcaa ggatatcgac ataagcaaag tctactccac
     4021 aagcctcgat acactgaaca gcacgagcgg aaaacccgca ttgaggcatc ttcggagtgc
     4081 ctttcatata gagcattatc gcattttcag caatttgctt tttaatcttt tctaaagtat
     4141 ccactatgca ccttttataa aataaaaaac ccaattatgt cgaaaaccaa acaaggtcgc
     4201 aactaatgat tctattgtga taatcctatc aatctttttc ttatagtgat attttaccct
     4261 acctcaacga tcaataaaat accaatttag tactaaatgt gccaaaaatc cccaccttga
     4321 cgagaattaa aatccactat tttgtaaaag attattatac cagcagaaat gtatccatgg
     4381 ccaagactat aaaaatattg aatcgctcag taatggcgca ttgaatatca tacttatcct
     4441 gataaactaa agacttttaa tcctggagat tgatatgact tttaccctgc cgcaattacc
     4501 ttatgcattg gatgcactgg ctcctcatgt ttccaaagaa accttggaat accattatgg
     4561 taaacaccat aatacttacg ttaccaactt aaataaatta attcccggaa cagaatttga
     4621 atccatgact ctggaagaga tcatcatgaa agctaaaggc ggcattttta acaatgcggc
     4681 tcaggtttgg aatcacacct tttattggca cagcatgagt cctaatggtg gaggcgagcc
     4741 aaaaggcagg ctggcggaag ccattaataa aagctttggt tcttttgcag cattcaaaga
     4801 acaattttca caaacagctg ctacaacatt cggttccggg tgggcatggt tagtccagga
     4861 tcagtcgggg gctttaaaaa ttatcagcac aagcaatgct ggaacaccaa tgactgaagg
     4921 attaaatgcc ttattaactt gtgacgtatg ggagcacgct tattacatcg attatagaaa
     4981 taggcgcccg gattatattg aagccttttg gtcattagtt aattgggatt ttgcgagcag
     5041 taatctgaaa taaaataaca agctggattt gttgcaatcc ctaccggata attttatgtt
     5101 tatggagaca tcatgagttt aattacaagt tacagcccaa tgcctgtaac cttcacgcac
     5161 ggagaaggcg tgtggttgta tgatgagcag gggaaaaaat acctcgatgg cttaagcggg
     5221 atagcagttt gtggattagg tcatgcacac cctgatgtca cccgtaccat acaggagcag
     5281 gcagccaggc taatacatac ttctaatgtc tttcatatta aagaacaaga attattggcc
     5341 gccaaactca cttcaatggc acgaatggag caggtttttt ttgctaattc tggagcagaa
     5401 gctaatgaag cagccatcaa acttacccgt ctgtttggtc ataaaaaggg aattgaaacg
     5461 ccttctatta ttgtaatgga aaaagcattt catggccgta ctatggccac cttaactgca
     5521 tcaggcagtc gtaaagttca agctggtttc gaacccttgg tccctgggtt tattcgtgct
     5581 cccttcaatg atcttgatgc cattcatact attgcggcca acagagagga tgtggttgca
     5641 gttatggtag aaccgattca gggtgagggg ggaatttatc ccgcagaaga aggttatctg
     5701 cgtgcattgg ctaacctttg cgaacagaat gactggatgt taattttgga tgaaatacaa
     5761 acaggaaatg gcagaacagg taagctttat tcttgtatgc attacgacat acaacccgac
     5821 attcttacca cggcaaaagg acttggaaac ggtatgccaa ttggggcttg tttaatgagc
     5881 aagcgagcta aagatttatt taaaccagga aatcatggct cgacttttgg tggtaaccct
     5941 ttagcttgtg caacggcttt gactgttctg gaggtcattg aacgagataa gctatgtgag
     6001 cgcgtcacta aaaacagtgc tttgttaatg gataaattaa ttagcaacct gggagagcat
     6061 ccccatgtaa aagccatacg tggtaaaggt tatatgatag gaatagagtt ggacaggcct
     6121 gcaaatgaca tgcgcgtcct gggtttggaa aaccaagtct tgttcaatat tacggctgaa
     6181 acagtagtaa gattattacc tccacttatt atcagtgaag atgaaattga tgaattggtt
     6241 aatcgaataa ctcgtactat taaccagttc accaatcagt agtgagaaga caactttctc
     6301 tcagttgaat agaaattgtt ttctccctgg ctgtctcgca gtatgttaaa ttaatctggt
     6361 atctgacagc atgctccctg gattatccgg gagcatgtgt gtgagttaca tcaaaacaca
     6421 aagctttgcc ccttggcaac aaaacctaag aaaccatcct ttttggcatt aggcaatttc
     6481 cctggattat aatgatatag ccacattttt ttctttaaat gagagggaag tttaacgagc
     6541 tctgagtagt gcgcgtgtac cccgctttta gattctgtag tttcacaatc atgaaaaata
     6601 atatctgatt cctcataaaa ggaaattaac tgtaccggac aactttgcgt gtctgatgta
     6661 aaaaggatgc gtgatttatt ataactaaat atcaaaccaa aacttggcat caattcatat
     6721 tcgctatagt aatggacagt ttgtaccaat ttaaacttta ttgattgcca aacaaaacct
     6781 tcatattgtt ttaccggtct aacatcaaaa aaagcgctaa gatttgctct ctcatgaggt
     6841 aaagtgctta acccgccgga taaactttta tcccataatt ccttaataat tttgtcagaa
     6901 gcgattaaac aaggtttgtg cgaatgatta ggatcaaaat aggttattag agcgaaccat
     6961 tccaatccgc cgcaatgatc ataatgcaag tgactaatat aaacattttt gatgtcttta
     7021 taactcaatc cctgatcccg taatgagtat ttgatatctg ttcctgcatc gattaataaa
     7081 gtatcgtttt ctagctctaa aagcacattg gattgatagt tttctggccc aatagcaaag
     7141 gacgatccgg aacctaaaaa agtaattttt agagtcattg taccccctgc cgtcactcac
     7201 taaaatgccc tgaccttaaa cctgctgtct taaggtacta atccttgcta catgataaaa
     7261 tagaactgtt aatttaagac tatgtgatga ttattgaatt gtcaaaaacg ggtaggagcc
     7321 attatcaaaa tagctacatt ctatttttta agtcatttaa atactgatta atggttattg
     7381 cctcattagt aaagagccat ttaacccctc tatttaattc tctatacaga ctgtttttgg
     7441 aatcaacaaa tcccacgcca aaatgttgat tggcatcttg taaatatttt aataccctat
     7501 tagtcaataa taaataatta cccagtacgc ctccataccc ctccttaatg cttaattcac
     7561 aaaatttctt aacgttatta tgcactgcaa caagcaataa agattgccta ggaaaagaac
     7621 acaatttttt aaaggtagat tcctgcaaag tcaataaatg aaagtcttta catggcgtta
     7681 aatgttgcag tgtttgcact aatatctctt cctcctcaac agctgttttt aattcaataa
     7741 ataaatgcat ggaattccca aactgacgca ctacttcaga gagggacggg attctgcact
     7801 ccaatgcccg taactcgcta aaactcagat cgctgatagc aatgtccttg ttccataatc
     7861 gatttaaggt aggatcatga ttaacaatga acaccttatc cttagtgagg tgtacatcca
     7921 gttcaactcc ccaacatccg gcctcttttg cgttctgaaa tgcttccaga gtgttttcaa
     7981 ttatccctct tgcattatta tgagctcctc tatgagcgat tactaacgca ccgttgatat
     8041 tttcgaattc cggtgcgtgc ctgggaatta cagcaaaata cccatcaatt aatttttcta
     8101 tgcatttgag taacaaaacc aattatccta atagtccaat tttaatcatt ttatgccggc
     8161 ataaaataaa aggcaaaata cgctattagc acaaaatcgc aaaaatcgac taaactagaa
     8221 actatgtgag aattacaata caagaataaa ttagcattta taaaatttgt aaataacttc
     8281 catcttaaaa tcaatgagtt gaattgaagt tatttagaaa tcctgattta aatttcttgg
     8341 ctacaaatat tcacaaggag tgttctaatg gcaatcaaag taacaaaaag gggaatggac
     8401 ttattaagag accccgtact aaataaaggc accgcattta gcctccagga aagagatgaa
     8461 tttgccttgc atggtttaat accaacaaca gtggaaaccc ttgaacaaca agtaattcgt
     8521 tgccttgatg cttatagtgc caaagaaaat cctttggaaa aacacattta cttacgtgcc
     8581 ttgcaggacc gaaatgaagt actcttttat cgttttataa ttgataatct ggttgatatt
     8641 ttgcccatta tatacacgcc agttgtcggc caagcctgtg aaatgttttc ccacatttat
     8701 cggcaaccaa gaggagtatt tctaagttat ccagagcgag acaaactgga cagcatcata
     8761 caaaacattg cgtcgacccg ttcaatcaaa gtgattgttg tcactgatgg cgaacggatc
     8821 ttggggttgg gagatcaagg ggcaggtgga ttgggaattc ctataggcaa attgtctctg
     8881 tataccagtt gcggtggtat acatccgtct aacactttac ctattattct ggatgtagga
     8941 accaataata aggagcgtct gggcgatccg gaatatattg ggtggagaca cgcccggatt
     9001 tcaggcaaag agtatgatga ttttgttgat caatttgtgc aaagcataaa acgccatatg
     9061 cctcatgtgc tattacaatt cgaagatttt gcacaacaac atgcctatcc tttattggag
     9121 cgatataaaa atcaactttg tacttttaat gatgatattc aaggaactgc ttccgttgct
     9181 gtagccgcca tactagcggc aactcgtgtt actaatactc ctttaaaaga acatcgaatt
     9241 gctttattag gcgcaggatc agcgggttgt gggatcagtg agcagctggt gcatgccatg
     9301 atgaaccagg gactttctga agaagaagcc agatcacgtt tttatttagt tgacaggtat
     9361 ggacttttac atgatgagat gacagatcta ttgccatttc aaaaaggatt tgtccgttct
     9421 tctaccagtc tccaaaattg gaaattagca aaaaaaggag aaatcacgtt aacagacgtt
     9481 attaacaatg cccaaccgac tatactcctg ggtgtgtctg gccaacctaa ccaattcaag
     9541 gaagcaatga tcaagaccat gctaagctac tgtgaacgcc ccataatttt tcctttatca
     9601 aatcctacgt ctcgtgcaga agcaatcccg caagatctgt taaattggac agctggtaaa
     9661 gctttaattg caacaggcag tccctttgag ccagttgtaa ttaatggtca taaaattgag
     9721 atagctcaat gcaataattc ttacattttc ccgggagttg gtcttggagt agttgcaggg
     9781 caagccaaaa gagtgactga tttaatgatg atggcagcag cagttgcatt aagtgagcta
     9841 gcacctgcaa tcagaaccgg agaaggacgc ttactcccag aattaaattc cattagagaa
     9901 gtcagtcaac atatagcgag agcagtaatt ttacagggta taaaagaggg tcatatagga
     9961 ccaatgaaca ataatgaaat cgatgattct ataaaaagaa cgatgtggac tccacaatat
    10021 gaaccatatg ttctataata aaacgatgta gaaattgctg gaatttaagt tttaattttt
    10081 tagaaaggct aaaatttatt agtttatcgc aatagaattt acagcatagg gttgagggga
    10141 aatcaggtta ttgatcctgc ctgattccct gcggtttatt tatagaagct tattcttatt
    10201 attaaattga gcttaaaaac agatagaaag cccctgaaac ataactattt tttatcgagc
    10261 cccttatatg tccataatta ctaccaatct caatcgggca attcatgatt tacagcaagg
    10321 aaaaccagta gctataccaa ctgaaacggt ttacggattg gcagcaccaa taaacaataa
    10381 tgctgccatc agagctgtgt ttgaaatgaa aagcaggcct ttaaatcatc ctctgattgt
    10441 tcatattgct tccaattggg atctgcatga cctcgtggaa gaggtacctg agtatgcaca
    10501 tcaattagct ggcaagtttt ggcctggacc attgaccctg gtattccatt gtaaaaaaga
    10561 tcaaatcaac ccgctcatta ccggtggtca atctaccata gcgataaggt gcccggatca
    10621 ccccttgaca caaaaattgt tacaaaaatt aaatattcct gtggtggcac cctctgccaa
    10681 tccttttgga aaaattagtc cgaccaccgc tttgcacgtt aaacaatcat ttaatgatgc
    10741 cgagttaact atattagacg gaggacgctg tcgtgtggga attgaatcaa ccattgtaga
    10801 tgctaccaat cctgattctt atcaaatttt acgccatggg ataatagatg agaaaactat
    10861 tgctgaaacg attgccatag gttgctcgac tcaaaaaaat gatattcgtg tccctggtaa
    10921 attggatagc cattatcaac cacaaaaaac tttatattat tttgatcaat atgaaaccct
    10981 ttccgaattt tgtcaaaaac gcaaagggac tgtttatgtc atagcgaata gacaaccccc
    11041 tggtgtcgag actaatctct ttcatccact gggaatgcat cctgaaaaat cggcctatga
    11101 tttgtattat caactaagat tagctgatac atccgatgcc atttgtatcg ctatcgagct
    11161 accacccaat acaagtgaat ggcaaggagt acgagaaaga atattaaaag ccgggttccc
    11221 ctattcgcag caataaatct cgagtaattt atactttttt gatttcgtgc aatagtgtat
    11281 tgacagaaca ctataatttg cgtaacttaa cgccctatgc cgaggtggtg gaattggtag
    11341 acacgctagc ttcaggtgct agtgggggca accccgtgga ggttcgagtc ctcttctcgg
    11401 taccattaat taagtaatga ttgccattgg tttacctaac tgaatcttgc taccagccaa
    11461 taattcttta tcccattgta ttttttctcc atttgcaaac aataaaacga ccgtagatcc
    11521 caatttaaaa taacccattt cacttccctg agatataatc ttgtccggaa actgctcact
    11581 ataatcaaaa cgttctaatt tttttgctct tttgacatca ccatgccaac tggttccaat
    11641 tgcgcctaca atagtagccc caaccatcac catcaccatc ggaccaattt tcgttttaaa
    11701 aaaaatggca agcctttcat ttcgtgcgaa caatttagga acaacacgtg tagttgctgg
    11761 ttgaacggaa aacaaagcgc ctggaatata agtcattgaa acgagctctg catctattgg
    11821 catatgtacc ctgtggtaat ctttcggcga taagtatagc gttgcgaatt gtccttgcac
    11881 aaattgttcg gccaattgcc cgtcacaagc taaaagctct tgtacagaat aatatttgcc
    11941 tttagcctgc aataactgtc ctcgttcaat cttgcctatt tcgctgatac aaccatctac
    12001 tggacaaatc acatctgact gagaaagagg acggcattcc ggctttaaat gacgaataaa
    12061 gaaatcatta aaacagtcat aagacttagg atcttcaata agtgcctcac tcatatcgac
    12121 atcaaactta cgaataaagt ttcgaattaa gaaattcttg agacgagggc tcttaacgtc
    12181 agcaaaatac cctgctaaag cagtaatacc gtgtttcgga ataaggtatt ggggtagtgt
    12241 cttcagtaca tcacgaaaca tactttttac tcaaaactga aaaaagagaa taataaccta
    12301 aagcttattg tattgtcaca ataagacgag taaatctcca tgccagagaa aatttaaacc
    12361 accaagctcg taacatttga attcaaagta gtttcttgcc tatgagatat aggttctgcg
    12421 taatttttga cctctttaaa ataatcagcc ctgtgttctt tggcattgta gcaattaatt
    12481 agcctgaatc ctggtatctt taatttttcc ttgtttttgt ttatgtcttt ttgtatggcc
    12541 gcgatgattt tatcaaataa aacatcattc agtgaggaag aatctttccc gttcaaaaag
    12601 gtcatagcaa caatttttgc ttgtgagaat tttgaacaat aaccttccac ctccgatctc
    12661 cttgaagtgg accaccataa tttaccttcg taatccttta atgcagactc aagcaacccc
    12721 ttaaacttag tcgcggggag ctcatcaatc cactcataca ttgttttgaa aaatagagac
    12781 ttaccctcca cattgtataa atgaataaaa cgcctgtcat gtttggcgaa atctacagct
    12841 gctttggtaa aaatttccaa atccttatca aaattacctt ctttaactgc attacgaatg
    12901 ctcaacaaag gataagtttg tagcatataa ggcgtgaggt gttgaataag caaagtattt
    12961 aaagcacctg tatcaccctc ccctgcgctt aaaatataag ccaatctgtt ttcaccactt
    13021 tcatttacgc tagcttgtaa atattcttta acaggttgtg ctctatgacg ctttgataac
    13081 agttctatag ttggggcata atatttatca attatttcag agatatgatg agataaatct
    13141 tcaggtctta agttcttggc ccaaaggaat aaagaatcat taaactgcct aatgacgtct
    13201 tcatgcgtat gtggcaatat ttctttgggt gtcgtggcta cagttgcttc cttcatctgc
    13261 tcatctgttg tcgttaaatg caattggaaa ttagcccgct ctgtgtattg ttttaaccca
    13321 acggctatgc gattaaattc accggcaaga gtgcttgtat gtgccaagct ttgacgaatt
    13381 ttcaggttat aattcgtata caactgtacc aattgctcgg agaattcaag attgtcctcc
    13441 tccgttaaat cctttcggtc tttctgataa taagtcttag ctactgcatg aaattgagtt
    13501 gtaaattcaa caagcaatat taacgctgtt cttagtttgc tgtctttatc tacactaatt
    13561 aatggggtaa ttttctcgag tgataactca ggcatggtgc caaacaactc ccaggcattg
    13621 gttaaggtgt catcggttgg ctttttgcct tctacttcat tcagaacaac gtgaaatgta
    13681 gacacttgct gcaatagctt attagtcagg ctataggagt catgcaacag atctctgaat
    13741 gtaggcgcat aagcatctcg ccacacctga tgatatcttc ttcgcaactc atcttcatca
    13801 aatttgatgc tgctgtcgtc cttgctaaaa atcgtaatat tttgagtcct tgcccattcg
    13861 actatgtctt tatagaaaga gggtttgtga tagagcgctg aattcgttgc aggcaaagga
    13921 actccgtacc cattattttc acaccccata taaaaaacca ctactcgata caaattatca
    13981 taatgcattt gatataaatt cattatgaaa tcgacgaatg gttttatatt ggtgctttca
    14041 ttgcataaat gaggaaatgt tttctcgtac tgactgcgca gggcgacatc cgtttcatcc
    14101 agagaagtgt agtttagtgt catctcacca aaaagctcgg tcaaacgttt tcttatcatc
    14161 tccggctgat aagttaacaa tattttcaac gcggcagcaa atttttgctc ctgagccact
    14221 ggctcatgag cgagttgctc aaattgacca ggatccgggt acgtcttttc aagaattaaa
    14281 ttaaccagct tatcttgaac aggcaaaacc gttggcaaag tttcctgtcc cggattttta
    14341 tatgttggcc aatgaaaagg cttcgagtct ttgacattag gaaatccttc ccaatcgcgc
    14401 acggtcaaat taacccttgt tttaggtata cctatagctg gcctcggctc tttcatgtag
    14461 atggtaaacc aataaaaaaa catatcaaaa tcaatgtccc cggccaaact tagattatgc
    14521 gggtgaccat catcatcatc aagaaaccac cttcccagaa gaatttccat gaaacttttt
    14581 tcaattaagg ttcttgtgct gggaatcacc tgttctttgg cggaagactc ctgaggcact
    14641 gactctttat ggaaattaaa ccctttaaat ccgtcaacac tgatagataa agtacctacc
    14701 agcctctctt catcatcaaa tacaagacgt tcttcggccg atcttcttcc ctgaaaaatt
    14761 cttttaaata aagagacagc gacacttatt ttcgctaaaa gttccggata atgattttta
    14821 ggatccagtt ttttataaaa ggcattttta ataacgccat tttcaataaa actgaccttg
    14881 tatgtttgat gcgagctatc cgaaaccgca gtccccgctg ttaaaaactg cagttggctc
    14941 tctttcagtg cttttttggg caaacccata tatcaaatcc tttaaatcag atcaatccat
    15001 cctatgcccc atattaatca gaaatagtca agaagttcaa gtataatttt catacaaaat
    15061 aacacttgac tagaaacaaa ctagacatct ggcacacata atgagttata aataaaaatt
    15121 tgcttgctta tttatcatga tgacaaaccc taaatcatca gtttaattaa acattcaggg
    15181 ttgtcttata ttgcccaaag cgataaggtt ttgttgacag aagccctatg attagggcga
    15241 aattgtaatt aactccggag aatcaatagt agttaatacc tctttattta aggctcttat
    15301 gacttctcca gatggacttt ggttaaaaaa ccaataattg ccagtggcac tttcttttaa
    15361 atcgttagca aaacccaagg tcagtgccgt aataataata ttagctataa cgactaaaac
    15421 tttatgtatt attgacttta tttcaggatc aacaatactc aatatttcct tttccgcact
    15481 tttcatttta atgtgcagat caacacctga ttttgttatg ccatcatagg caatgctgta
    15541 gagcgtttgc cggtattttt catcagcacg ctgccaattt ccaactttat ctttatcaag
    15601 agatgatttt cgtaatcttt catgtatatc cttgcatttt tcttctaccc gcataatgac
    15661 atgcctgaaa cggctggagt tgatattgtt ttcttcgcct gtaaatgcaa tgatctcttt
    15721 tttaaatcct aaatcttgca tctgctggac acaaataaat cgttcgcgca acgctcgtgc
    15781 caaaactaat aaattgctgt ctttaatttg taaaagcgcc tttccctgag taacaacccc
    15841 tttttgagct cctatatata aaagttcaat taaagctttt ctatgagaga gcgattcgat
    15901 tttagcgagc cctggcaaaa acaagcgaag caattgccct gttttattat tttttagcac
    15961 caaacgataa gcataatcat tggcaatccc cgcttctttc aagactttaa aagatttccc
    16021 taaagcatcc agatcatcca aatttaattg ggataaatcg gatttacgca aattgtaggc
    16081 tttaaattgt tcagagtaat gatataaaat agattcctgt tgatgttttt tgggatccag
    16141 agccagtttt tttagctgtt ttatagaaat ggttttggtt ttatctaatg ctatcaatgt
    16201 ttcagccagc attgggtaat ggctggtagc gttaactatg tcttgtattt ctttgatcga
    16261 taaactcccc ttgacccaaa atataagaca tagttttctg caatgatcat ttgctaaagt
    16321 attaatgaat tttagttgct ctcttttttc ctgatgggct aataattcag ggatatcttg
    16381 agttaaacca aaatcgacaa ggacatgaag cgaacgataa tacgcttcgt caaataagag
    16441 ctctttaatc tcacttaaag tgaaattttt ctgaattaga aaaggaatta acttgatttg
    16501 gatgtcatcc cacatcaatg cgcttaattg ctttattaac tcatgatctt gcaataaaga
    16561 acgattttct tccaaagtat tttcttcata aaatacaata atcatttgca aaatactttt
    16621 atacagagtt tcctcagagt taaaaggaac cgcttcaagc tccttcttta atctgctttc
    16681 ttccgacaat aaatcgtttt ccatggcggg agtcaaacga agcttgtatt gttttaccaa
    16741 ctcctgggct gtaatactca ttggaagtta acctcttgtt catttactaa taaaccctat
    16801 ttcatggaga tagggtaata acgattattt tttggatc
//
LOCUS       DATNSN010000063        16770 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.063, whole genome shotgun sequence.
ACCESSION   DATNSN010000063 DATNSN010000000
VERSION     DATNSN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.063"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(4..1743)
                     /locus_tag="VAH76_001649"
     CDS             complement(4..1743)
                     /locus_tag="VAH76_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1386276.1"
                     /translation="MKTSNSQNGTSNELSLNLGKLSTAKNLVFRGGGSKGVAYVGALQ
                     SLSEQGVLENIENVAGSSAGAMTAAIVACGGSAELMDYFCSANSLADFVDNRLTRKLL
                     PKREKSPQADSSVDYSSISHEQTGNQLDTKSSLAEAKLATKPSKLTFAVASKGAQLVE
                     AMEQVMRMAIFTHFPESLDNELKSVREKLNAIEDKGSAKYTNLAKRQQILEDTQKIIN
                     GLHDPNFDLTFGHIHTLHQYDPQRFKELYVTGTDEKGELQIFSHEKDVDMPVSIAVRI
                     SGSLPGVFDPVIYKGRKYIDGGAANNLPVNIFFNKEDAYTPGVVTTIGVSCENQLDIT
                     EKEFENPSFMERIMGYLRVFVKKLLCKLDVEGAVKEQKREMLKYIDNGNLFVMVAPPI
                     SITTAQMHIDKEEKEGVIKVGKAFCDVQLKSMIDADYKPQPLDEKTAKILASSGEQQE
                     HSIQKNFDFKSRLQNFKQADRPWSQHVEKTIDRLMLVKGEATQDQIDLCLQWQKIKSV
                     HSNPADRLREFAYAFLASPLAEPVSNHQEINFSKETRKEINLLKNLPGLYETTPDFTE
                     EFNNQRMSSLQRA"
     gene            complement(1939..3174)
                     /gene="flgL"
                     /locus_tag="VAH76_001650"
     CDS             complement(1939..3174)
                     /gene="flgL"
                     /locus_tag="VAH76_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEO1386277.1"
                     /translation="MRISTNQIYQRGLDNLLTQQERLARLQDQQASGIRVRTPSDDPV
                     ASAQIELIKQRISNIELLQNNRQAADSALRLEEGILSNTVNSLHRLREIQIQSGNPSL
                     SEEDRKTLAVEAQALLNQLLDYANTKDSNGSYMFSGSKSLTQPVSLNLSGQYVYNGDN
                     TQRFQAVTTSLLVAVNDTGDNVFMRIPSGNGRFAIRETVTPNTGTASVSSGSVINEAA
                     FVPDNYTMTFALNSQGNLVVMVSGTLSGNVIPPSGLPDDAPLYQEGSAISFNGMEMVV
                     SGLPKAGDSFSISPAKNESIFSTVQRMINNLNKPYVSSVEKAATQTENNQLLAQIDSA
                     LGHILSVQSDLGARLNQLETAEKANNDYLDISAATLKKLREIDPVAVATELNLQLVNL
                     QAAQLSFTRIQGLSLFNFL"
     gene            complement(3178..5127)
                     /gene="flgK"
                     /locus_tag="VAH76_001651"
     CDS             complement(3178..5127)
                     /gene="flgK"
                     /locus_tag="VAH76_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEO1386278.1"
                     /translation="MSILNIAYSGLNAFQRALDVTGNNIANFKTRGYSRQSIQFTPIA
                     SNRYAGSYIGAGVSVSSIYRNVDQFANAQVRSTLSYRTQYDAFYNQAIQIDKLLSQDG
                     SSISVPLQTFFDSIGQLNSTPDNIATRGVVLKQSQLLAQQFNSLQTKLEEYERNSTLQ
                     VTESVKIINRITKELAEVNGKLLGNNNIPELLDHRDELLKQLSGYTDLSIFDQGDGTI
                     SVGIASGDMLVAGTQQRDLVVGTGKDSIFGTKIFLSSGGNNQVDITDRLTTGMLGGLI
                     DYEKNVLGQASQLIGQMAIGLAQTFNAQHKLGMDMNSQIGKDFFTDFNSPGQMLKRST
                     ASADNSGTAVLSVNISDISQVKLSDYDLIISDTGANELRLIRKSDGTSTTLTWSSSPP
                     APPAGQVVIDGMTITVNDLSQLANNDHYTLTPTRGAARDFALEIKDAYEIALASPVKT
                     TASLNNTGQGQIILGPVLNTASVNKQFRIDFISDTQYNLVNVTDSTTAGPFAFVPNTN
                     NVIQIPDGVTPSYSVVLSGIPKSGDQFTTDYNAGGFGDNRNGLILSNIQQNKIFSNGS
                     ETLFDRYGGLLAEVGGRTNQAKTSFESADILHKQALDFQDSKSGVNLDEEGANLLVFQ
                     QAYQAAGKLMEISNQIMNLLFDIMR"
     gene            complement(5177..6052)
                     /locus_tag="VAH76_001652"
     CDS             complement(5177..6052)
                     /locus_tag="VAH76_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEO1386279.1"
                     /translation="MTIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSM
                     LKSMRMGQHFLDESSPFSGKNEATFQEMLDTQYASTIAESKGIGLAALLAKQLENSVG
                     DKANNLVNSSTEVSNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVAQAA
                     LETGWGKFVTRDTDGSSSNNLFNIKAGSHSEVESIQVKTTEYIADTPIKINASFRKYP
                     SIEHSFHDYVSLIKGSERYQMALANAENPEIYVSELNKAGYATDPNYSNKILSIYHGD
                     ELNQAIQRCESSEQS"
     gene            complement(6064..7167)
                     /locus_tag="VAH76_001653"
     CDS             complement(6064..7167)
                     /locus_tag="VAH76_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEO1386280.1"
                     /translation="MRRMLVIRWILAIHLIATQVFAERIKDIATLAGVRVNQLVGYGL
                     VVGLSGTGDKTGTKFTEDSFANMLTQLGINVPPGVRLNSKNIAAVMVTANLSSFMKKG
                     QTMDVNISSIGDSKSLLGGTLLLTPLKGADGRVYAMSQGNVVVSGISASGSDGSSVTV
                     NVPSGGRIPNGATIEADIPNPFYYSNSLTYNLHTPDFTTAKRMSDAINELMGPGTAKA
                     IDAGSVVVTAPKKLSQRVDYVSVLENIEFKPGEAMAKIIINARTGTVVISSNVIVKSA
                     AVSHGNLVVSITETPVISQPNAFASGRTVATQQSQVNIQQKNNRAFILPKGTTLKDIV
                     RGINAVGATPADVISILEALQQAGALSATLIVI"
     gene            complement(7206..7898)
                     /gene="flgH"
                     /locus_tag="VAH76_001654"
     CDS             complement(7206..7898)
                     /gene="flgH"
                     /locus_tag="VAH76_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEO1386281.1"
                     /translation="MNRLNIAVSCLATALLFGCEALHPPAPGDNPDYAPTYPVTPDPK
                     ELRKVSGAIYSSETALPLFETPRARHPGDILTVYLIEKTDAQKNATTTQRKNDTTKIT
                     NKLFLGRPISLGSGYSMDFDLDNQRQFNGEGRSIQNNKLAGSISVTVAKVLANGNMVV
                     QGEKWVRINQGNEFVRLSGIVRPQDIKADNTITSDRIANARISYGGTGQINNTNAQGW
                     LSRILWGPLFPT"
     gene            complement(7911..8696)
                     /gene="flgG"
                     /locus_tag="VAH76_001655"
     CDS             complement(7911..8696)
                     /gene="flgG"
                     /locus_tag="VAH76_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEO1386282.1"
                     /translation="MEPALWVSKSGLEAQDKNIATIANNLANVNTTAFKKGRALFEDL
                     IYQNLRQAGAQSSQNSQIPTGINMGTGVHLVATEKIFSQGSLQNTQNALDVAIEGRGF
                     FTVLMPDGTQAYTRDGSFKTDSTGQIVNSNGYPLQPNITIPPQTTKITIGTDGTVSAL
                     VAGNNAPSVIGNIQLTDFINPAGLQPIGQNLYTETVASGTPITDNPGNSGLGTLLQGS
                     LEASNVNVVEELVNMIQAQRSYEITAKSIQTVDSMLQYLTQTL"
     gene            complement(8857..9603)
                     /gene="flgF"
                     /locus_tag="VAH76_001656"
     CDS             complement(8857..9603)
                     /gene="flgF"
                     /locus_tag="VAH76_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEO1386283.1"
                     /translation="MEPILYKAVSGGRSDFKRQEMIANNLANINTPGFKADLYQAQTM
                     YMNNANGNNQFNMHSFITQNANGVDTSPGEIITTGRDLDVAIDGDGWMAVQDSQGREA
                     YTRGGSLRLNANGQLITASGKVVLGDGGPISIPPAQSIEIGSDGTISIVPLEGDVKSL
                     AILDRIKLVTLDRNNIYKNDEGLIQLKSGAATANSNIKLQSGALEGSNVKAIDQMVAM
                     ISAGREFDAQMKLLSTVDDNGQKLAQLLQE"
     gene            complement(9614..10927)
                     /gene="flgE"
                     /locus_tag="VAH76_001657"
     CDS             complement(9614..10927)
                     /gene="flgE"
                     /locus_tag="VAH76_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEO1386284.1"
                     /translation="MVFNTALSGIQASTRDLEVIGNNIANSATIGFKGSRAEFADIYS
                     SSAYGSGGNAVGGGVKLSRVHQSFATGTLSTSNNTLDLAVNGSGFFILSDQGAKVYTR
                     AGQFKLNNENYIVNANNQRLTGLLTDSNGAITGASGDLRINTSNITPRASSNVSVGVN
                     MNSQSKAPNVDWVGGASPATDTYNNVTSSTIYDSLGNSHVLSMYFIHADPTALAGSPN
                     AASPAGTENQWYVAFQIDNQNVPPNVGAQNTNNLFRANFNSDGSFAGVFDTSNAALPN
                     NLIPLSFNLNNGSSPLNLNIDLSDSTQFGSPFAVQSAYNNGYTTGSLAGLDIDDSGMI
                     YGRYTNGQSLAMGQIQLANFADPESLQNIGNTSWTETTSSGQPLVSAPGSGGLGLINS
                     GRLEESNVDLTGELVKLISAQRNFQANAQTIRTGDAITQTIINIR"
     gene            complement(11041..11718)
                     /gene="flgD"
                     /locus_tag="VAH76_001658"
     CDS             complement(11041..11718)
                     /gene="flgD"
                     /locus_tag="VAH76_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEO1386285.1"
                     /translation="MTTNSVNGSNSAGGLGLNQIDSDKRQNLGQQDFLRLMVAQVQNQ
                     DPLQPQANGEFLSQLAQFSTNDGITKMQESLQQLASSLQSNQALQASALVGRKVLVNS
                     NTLSLGTEGYVKAAIDMAPGVSNLRAAIYTESGELIKTIPLGQPEPGFFQFSWDGTDQ
                     SNQRLASGRYTIKVTGTYGGQEVALKTMTSANVDSVSLGQNGEDLKLNVAGVGSVYLS
                     QVRQISV"
     gene            complement(11729..12151)
                     /gene="flgC"
                     /locus_tag="VAH76_001659"
     CDS             complement(11729..12151)
                     /gene="flgC"
                     /locus_tag="VAH76_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEO1386286.1"
                     /translation="MSLNAIFDIAASAMTAETVRLTTSAGNMSNANVEAGSPDEVYQA
                     KYPVFKSVQENANQWMGEQIKAGVKLDGIYESDAEPHRRYDPNSPIADKDGFVYTSNI
                     NYVEEMANIISASRSYQMNIELLNTTKQLMQRTLQLGE"
     gene            complement(12154..12546)
                     /gene="flgB"
                     /locus_tag="VAH76_001660"
     CDS             complement(12154..12546)
                     /gene="flgB"
                     /locus_tag="VAH76_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEO1386287.1"
                     /translation="MAINLDSYFGIHAKALIARDQRASVLANNIANVNTPNFKARDVD
                     FNEVLTATMAGNSQQLRVTSDQHINTNADFMTHLKYRMTHHASLDGNTVDKDMETTEF
                     ARNALNYQASLSFLNGKIKSMLTALKGE"
     gene            12762..13697
                     /gene="hemF"
                     /locus_tag="VAH76_001661"
     CDS             12762..13697
                     /gene="hemF"
                     /locus_tag="VAH76_001661"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEO1386288.1"
                     /translation="MSISKTLPYNAIEQIKSYLLQLQNTICVSLESIDGKTRFHEDSW
                     QRAAGGGGKTRIMANGNVFEKAGVNFSHVSGEQLPASASAHREELAGRHFSALGVSLV
                     IHPQNPYVPTTHANVRFFVAEKEDSEPVWWFGGGFDLTPYYGFVEDCEHWHQTALNAC
                     LPFGETIYPKFKRWCDDYFFIKHRNEARGIGGLFFDDYNEISFDHSFELMRSIGDHFI
                     LAYEPIVARRKDIPFGNREKAFQNYRRGRYAEFNLVYDRGTLFGLQSGGRTESILMSL
                     PPIVHWEYNWHPEKGSDEEKLYTDFLPAKDWLKKE"
     gene            complement(13842..15149)
                     /locus_tag="VAH76_001662"
     CDS             complement(13842..15149)
                     /locus_tag="VAH76_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386289.1"
                     /translation="MTAGFIGLLKQRKFLPLFLTQFFGAFNDNAYKLAMLTMISYHLS
                     HTQEQSEYYQAIAGALFILPFFIFSATSGQLADKYDKALLTRLVKVLEVLLMIIGGIA
                     LYSGSIFLMMCTLTGMGVHSSFFGPIKYAILPDHLPKKNLLAATGLIEASTFIAILLG
                     TTLGTLTVGGKEAIPYIAIIMTLSAAFSGLVSSLFIPPAPSALHELKVDFNLWRATVK
                     MLKEAKKEYGILISILTISWFWLIGAVMLTKLPDYTHYVLGASAHVFALFLALFSIGI
                     ALGSITINWLLKGQVNLSAVPMAMLAFTFFAFDLYWSSPAGHDLKTPLFNLFNFFTSF
                     THWRIAFDLFMLAFCSGLFVVPLYTYLQIISPVENRARTIAANNIFNALFMVMGTLLV
                     MLLLFFSFSIPQVFLILCLLNLVAAVFLFLGLRTLRRSSEEIA"
     gene            complement(15386..15949)
                     /locus_tag="VAH76_001663"
     CDS             complement(15386..15949)
                     /locus_tag="VAH76_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1386290.1"
                     /translation="MVADLMLCTNFFIFTGGPGSGKTSVLNELNTWGYLTVSEVARSI
                     IQKQQADGGDAIHTGNRKAFRDLMLEQSIADYCKMQSEEKIVFFDRGIPDLYGYSKAF
                     CGEASKKVNEAVRRFRYNKTAFIFPPWPEIYENDDERQQDYQEALQTYYAVKEGYFDC
                     GYVLLEVPKCPIKDRANFIFQMISKKG"
     gene            complement(16007..>16770)
                     /locus_tag="VAH76_001664"
     CDS             complement(16007..>16770)
                     /locus_tag="VAH76_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386291.1"
                     /translation="RIVNSLINDELVTVNEGLDKRERFLRVTDKGKEKVNYIDEYSNT
                     KIKRAFHYLTDEEKEQVITAIGKYAQALEQSRLIRDQIRILRLSTSRALRKQIVNMIE
                     NIQVNEFHLSITPEINASILKAEEEYCYYSSCNFWYAVDEQGGIIGSIGLKKLNHTEG
                     EVKKFFIAPKYRGLGLAQKLMLTLVRNAMKHGFKKLFLGTVAQLNAARRFYEKNGFVL
                     VNKNSLPKEFELCPLDTHFYYCETDKLDKSLRELI"
BASE COUNT         4957 a         3580 c         2949 g         5284 t
ORIGIN      
        1 tttttatgcc ctttgtaagg atgacattct ctgattatta aactcttctg taaaatcagg
       61 agtagtttca tataatcctg gcagattttt taatagatta atttcttttc ttgtttcttt
      121 actgaaatta atctcctgat gattactgac tggttctgcc aaaggagaag caaggaaagc
      181 ataggcaaac tctcttaatc tatcagcagg gttgctatgt actgatttta ttttctgcca
      241 ctgcaagcat agatcgattt gatcttgagt ggcctctcct ttgaccaaca ttagtcgatc
      301 aatcgttttt tctacgtgct gagaccaagg tctgtctgcc tgtttaaaat tttgcaatct
      361 tgatttaaaa tcaaagttct tttgtatcga gtgctcttgt tgctcaccac tgcttgccaa
      421 tatttttgct gttttttcat ccaatggctg tggtttataa tctgcatcaa tcatactttt
      481 tagttgcaca tcacagaacg ctttacctac cttgataact ccctcttttt cctccttatc
      541 aatatgcatt tgggcggttg ttatggaaat tggaggcgcc accataacaa acagatttcc
      601 attgtctatg tatttcaaca tttctctttt ctgttccttt acagcgcctt caacatcaag
      661 cttacataat aattttttta caaaaactct taaatatccc atgatccttt ccataaagga
      721 tgggttttcg aattcttttt ctgtaatgtc taattggttc tcgcaagaaa ctccgatggt
      781 agtcactact cctggagtat acgcgtcctc cttattaaag aagatattaa ctggtaaatt
      841 attagctgca ccgccatcaa tatattttct tcccttatag attaccggat caaaaacacc
      901 tggcaatgac ccagagatac gaactgcaat agaaacaggc atatcaacat ctttctcatg
      961 agaaaaaatc tgcaactcac ctttttcatc tgttccagtg acatacaatt ctttgaatcg
     1021 ctgaggatcg tattgatgta gcgtgtgtat gtggccaaaa gttaaatcaa aattcggatc
     1081 atgcaacccg tttatgattt tttgggtgtc ttccagaatt tgttgtcttt ttgcaaggtt
     1141 agtatattta gcgctccctt tgtcctcaat tgcattaagc ttctcgcgaa cacttttcaa
     1201 ttcattatcc agagattcag gaaaatgcgt gaaaatagcc atcctcatga cctgttccat
     1261 cgcttcaact aattgagcac ctttagaagc aacagcaaaa gttaatttgg atggtttcgt
     1321 ggcaagtttt gcttctgcaa gactggactt agtatctagc tgattacccg tttgctcatg
     1381 acttattgaa gaataatcca ctgaagagtc agcttgtgga cttttctcgc gtttaggaag
     1441 caattttcta gtcaaccggt tatcaacaaa atccgctaaa ctatttgcac tacaaaaata
     1501 gtccattaac tcagcagatc caccgcaagc gactatcgct gcggtcatag caccagcaga
     1561 gcttcctgca acattttcaa tgttttccaa tactccttgt tcagacaagg attgcaatgc
     1621 tccaacataa gctacccctt tagagcctcc tccgcgaaat acgagatttt ttgcggtact
     1681 caattttccc agatttagag ataactcatt tgatgtacca ttttgactat ttgacgtttt
     1741 cataatttca aatgctcatc gggctcttta atacataata acaagtgaat atgaacagga
     1801 tgtgacatgc ctatgagttc tttttatagg ttataactta tagttatgtt gattgattta
     1861 caataatgag taagtctgca taagattatt caacactact tgatgtaaaa gataaaaaac
     1921 tctatttttg atatgctttt acaaaaagtt aaataatgat aagccttgaa ttcgtgtaaa
     1981 acttaactga gctgcctgga gattgactag ttgtaaattc aattccgttg caacagcaac
     2041 aggatcaatt tctctgagtt tttttaatgt cgcagcgctg atatcaagat aatcattatt
     2101 agctttttca gcagtttcca gttgattcag tcttgcgccc aaatcagact gtacgctcaa
     2161 aatgtgacct aaagcgctgt caatctgagc taataattga ttattttcag tttgagtagc
     2221 tgctttttct acagacgaaa catacggttt atttaagtta tttatcatgc gctgtacagt
     2281 agagaaaata gattcatttt ttgctggact aattgaaaat gaatcccctg ctttgggtaa
     2341 gccagacact accatttcca taccattaaa actaatggca ctaccctcct gatataatgg
     2401 tgcatcatcc ggtaaaccac ttgggggtat cacattacca ctgagagtac cactcaccat
     2461 gactaccagg ttaccctgag aatttaaagc aaaagtcatt gtgtaattat ccggcacaaa
     2521 agcggcttca ttaattaccg aaccggaact gactgaagct gttcctgtat taggagttac
     2581 cgtttctctt atggcaaaac gcccatttcc acttggaatg cgcataaaca cgttgtcacc
     2641 agtatcatta actgctacca gtaaactcgt tgtaactgct tgaaaacgct gtgtgttatc
     2701 tccgttataa acatattgcc ctgagagatt caatgacact ggctgtgtta gtgatttact
     2761 cccactgaac atataacttc cattactatc tttagtgtta gcataatcaa gtaattgatt
     2821 taataaggct tgagcttcaa cagcgagggt ttttctgtcc tcttcggaaa gacttgggtt
     2881 tccagactga atttgtattt cacgtaagcg gtgaagtgaa ttgactgtat tggataatat
     2941 cccttcctct aaacgtaaag cactgtctgc cgcttgccga ttattttgta ataactctat
     3001 attgctgatg cgctgtttaa tcagctcaat ttgtgccgat gccactggat catcagatgg
     3061 agtccgaact cttataccgc tggcttgttg atcctggagc cttgccaatc tctcttgctg
     3121 agtcaacaaa ttatccaaac cccgctggta tatctgatta gttgaaatac gcattcatca
     3181 cctcatgata tcaaacaaca agttcataat ttgattggat atttccatca attttcccgc
     3241 tgcctggtac gcttgttgaa agacaagcaa attggcccct tcctcatcca ggttgactcc
     3301 acttttgcta tcctggaaat ctaaagcctg cttatgaagt atatcggctg attcaaagct
     3361 ggttttagct tgattggttc tgccccctac ttcagcgagc aagccaccat atctgtcaaa
     3421 taaggtttcg cttccatttg aaaaaatctt gttttgttgt atgttactaa ggatcaagcc
     3481 attgcgatta tcaccaaatc caccagcatt gtaatcagta gtaaattggt ctccggattt
     3541 agggattcca gataaaacaa ctgaataaga aggagttact ccatcgggaa tttgaatgac
     3601 attgtttgta tttggtacaa aagcaaacgg accggctgtt gtactgtcgg ttacattaac
     3661 caggttgtat tgagtatccg aaatgaaatc tatcctgaat tgtttgttaa cagaagccgt
     3721 attcaatact ggtcctaaaa taatctgacc ttgcccggta ttatttaatg acgccgttgt
     3781 tttcactgga gaagccagcg caatttcata agcatcctta atttccaagg caaaatctct
     3841 ggctgctcct ctggtaggag ttagagtata atggtcatta tttgccaatt gcgataaatc
     3901 gtttactgta atcgtcattc catctataac tacttgccct gctggggggg ctggtggcga
     3961 gcttgaccag gttaaggttg tagaagtacc gtctgatttt cttatcaatc gcaattcatt
     4021 agccccagta tcgctaatta tcagatcgta atcgcttaat ttcacttggg atatatctga
     4081 gatattgaca gataagacag cagttccact attatcagct gaagctgtag aacgtttcag
     4141 catttgtccg ggggaattaa aatcagtgaa aaaatccttg ccaatttggc tattcatatc
     4201 cattcccaac ttatgttggg cattaaatgt ctgagctaaa ccaatagcca tttgacctat
     4261 taattggctc gcctgtccca aaacattttt ctcataatct ataagaccgc ctaacatgcc
     4321 ggtagtcagc ctgtccgtaa tatcgacttg attatttcct ccgctgctta aaaagatctt
     4381 tgttccaaaa atagagtctt tacctgtacc cactaccaaa tcgcgttgct gggttcctgc
     4441 cactagcata tctccgctgg caattccaac actaatcgta ccatcaccct gatcaaaaat
     4501 actgagatca gtataaccag ataattgttt gagcagctcg tctctatggt caagtaattc
     4561 gggtatattg ttattcccta atagtttccc attgacctca gccaactctt ttgttattcg
     4621 attaattatt tttactgact ctgttacttg caatgttgaa ttacgctcat actcttcaag
     4681 ttttgtttgt agcgaattaa attgctgagc taataactga ctctgtttta gtaccacacc
     4741 tcttgtcgct atattatcgg gtgtactatt caattggcct attgaatcaa aaaaggtttg
     4801 taaaggaacc gaaatacttg atccatcctg tgaaagcaat ttatctatct gaattgcctg
     4861 attataaaac gcatcatact gagttctata actcaaggta cttctaacct gagcattggc
     4921 aaattgatca acgtttcgat aaatcgatga aacagatact cctgctccta tgtatgaacc
     4981 agcatagcga tttgaggcaa taggtgtaaa ttggatagat tgtctgctat accctctggt
     5041 tttaaaatta gcaatattat tgccagtaac atctagggca cgctgaaaag catttaatcc
     5101 tgaataggca atattaagta tagacatatt ttgttcactc cctgaactaa ttttttcttt
     5161 aacttgggcc taactatcaa ctttgttcgg atgattcaca tcgttgtatt gcctggttca
     5221 attcatcacc atgataaata gacaaaatct tattactata atttgggtca gtagcatacc
     5281 ctgctttatt aagctcactg acataaatct ctggattttc tgcattggcc aaggccatct
     5341 ggtatcgctc acttccctta attaatgaaa catagtcatg aaaactatgc tctatggatg
     5401 gatattttct aaaggaagca ttgatcttaa tgggggtatc ggcaatgtat tctgttgttt
     5461 taacctgaat tgattctact tcgctatgcg acccggcttt tatattaaat aaattattac
     5521 tgcttgaacc atctgtatct cttgtaacaa atttacccca gccagtctct aatgccgctt
     5581 gcgctaccaa cagcttggga tccaatccaa ttaatgatgc agcctgtttt gctgtgggcc
     5641 acacagattt tacgaaatca tcaacaacag atagcgattc ttctgaatta gtcactttag
     5701 tattgctaac ctctgtagaa gagttaacta gattgtttgc tttatcccca actgaatttt
     5761 ccagttgttt agccagtaga gctgccaatc ctattccttt agactcggca atagtactag
     5821 catattgtgt atccagcatc tcttgaaaag ttgcttcatt cttaccacta aaaggacttg
     5881 attcatcaag gaaatgctgc cccatcctca tgctttttaa catcgattgc aagaatattc
     5941 cttcaaactg tttagctact tcaggtaacg cttcttttgc attgtttttt gcctggactt
     6001 ttaattcatt taatccttga aaatcactcg tagcaatact ttgtatggtc atatccatca
     6061 cctttatatg actattaatg tagcactcaa tgctcctgcc tgttgtaaag cttccagtat
     6121 ggaaatcaca tcagcaggtg ttgcaccaac agcattgatc cctctgacta tatctttcaa
     6181 agtggtaccc ttgggcaaga taaaggcacg attatttttt tgctgaatat tgacttgtga
     6241 ttgctgcgta gccaccgtgc ggccagaagc gaaggcatta ggttgactga taacgggagt
     6301 ctctgtaatg ctaacgacca aattaccatg agatacggca gcagatttaa caatcacatt
     6361 gcttgaaata accactgttc cagtcctggc attgataata atttttgcca tggcttcgcc
     6421 tggtttaaat tcaatattct caagtactga aacataatct actcgttggg ataatttttt
     6481 aggagctgtt actaccacag aaccagcgtc tatagctttc gctgtgcctg gtcccattaa
     6541 ctcattgatg gcatcactca ttcgtttcgc tgtagtgaaa tccggggtat gcaagttgta
     6601 agtcagtgag tttgagtaat aaaatggatt aggaatatca gcttcaatcg tagcgccatt
     6661 gggaatcctt cccccacttg gcacgttaac agtgacactt gaaccatcac tcccggaagc
     6721 actgatgcca gatactacaa cgtttccctg tgacatggca tagacacgac catccgctcc
     6781 tttcaaagga gtaagtaata aagttccacc taataagctt ttagaatcgc ctatggatga
     6841 aatgttgaca tccatagtct gtcccttttt cataaagctg gacaaattgg cagtgaccat
     6901 cactgctgca atgtttttgg aattgagtct aactccagga ggaacgttaa ttcctagttg
     6961 agtcaacata ttagcgaaac tgtcttcagt aaatttcgtg ccagttttat cccctgtacc
     7021 gcttaaaccg actaccaacc catacccaac caattggtta acacgaacac cagccaaagt
     7081 agcgatgtct ttgattcgtt cagcaaaaac ttgagttgca atcaaatgaa ttgccaatat
     7141 ccatcgaata accagcattc gccgcatctt aattccttgt cgtttgtaat ctcgataatt
     7201 accaattaag tcgggaataa aggcccccat aaaatccttg ataaccatcc ttgagcattc
     7261 gtattattaa tttgaccagt tccaccgtaa gaaattctcg cattggcaat tctgtcagaa
     7321 gtaatagtgt tatctgcttt tatatcctgg ggacgaacta tccctgaaag acgaacgaat
     7381 tcattgccct ggttaattct gacccacttc tccccttgaa caaccatatt gccattcgct
     7441 aacactttgg ctaccgtcac tgaaatactg ccagctaatt tattgttttg tattgatctg
     7501 ccttctccgt taaactgcct ttggttatcc aaatcaaaat ccatgctgta accacttccc
     7561 aaagagatag gtctacccaa aaagagttta tttgtgattt ttgtcgtatc attttttctc
     7621 tgagtggtag tcgcgttttt ctgggcatca gttttttcga ttaaatacac cgttaatata
     7681 tcacccggat gccttgccct aggtgtttca aacaaaggga gagccgtttc actactataa
     7741 atagcaccac ttacctttct taattccttg ggatccggag ttacaggata agttggagca
     7801 taatcagggt tgtcgccagg tgccggtggg tgtaaggctt cacacccaaa caacaaggct
     7861 gtagctaaac aagagacagc tatatttaat cgattcataa cctatctcac ttacagagtt
     7921 tgggttaaat attgcaacat gctgtcaacg gtctgaatgc tttttgctgt tatttcataa
     7981 cttctttgag cctgaatcat attaaccaat tcttcaacca cattcacatt agaagcttct
     8041 aatgaacctt gcaataaagt acctaatccg gagttgccag ggttatctgt aatcggtgta
     8101 ccgcttgcta cagtttccgt atataaattc tgcccaatgg gttgcaaccc cgctgggttt
     8161 ataaaatcgg ttaattggat atttcctata acggatggcg cattatttcc tgcgactaaa
     8221 gcactaaccg tgccatctgt tccaatagta atttttgttg tttgtggcgg tatcgtaatg
     8281 ttgggctgaa gaggataccc attactatta acaatctggc ctgtactatc agttttaaag
     8341 cttccatcac gtgtataggc ttgtgtgcca tctggcatta agactgtaaa aaagccacgt
     8401 ccttcgatag cgacatccaa cgcattttgt gtattctgaa gactgccttg gctgaatatt
     8461 ttttctgtag caaccagatg cactcctgtc cccatattta taccggtagg aatttgtgag
     8521 ttttgtgaag attgagcgcc agcctggcga agattttgat aaattaaatc ctcgaataaa
     8581 gctctaccct ttttaaacgc agttgtgttg acgttagcaa gattattcgc aatagtagca
     8641 atattcttat cctgggcttc caaacctgat ttgctcaccc ataatgctgg ttccataacg
     8701 gcctcctgct attattctta ttattatatc ggccttaatt atcatttcag attttcaaat
     8761 gcataaagca caccttccgt tcctaaaatc acctgtgaca actaattatt ggaaaacggt
     8821 tcgccctcag tgaaacaata attaaattaa ttaagtttac tcttgcaata gctgtgccaa
     8881 tttttgtcca ttatcatcga ctgttgataa caacttcatt tgggcatcaa attcacggcc
     8941 agcactaatc atagctacca tttgatcaat cgcttttaca ttactccctt ccaatgcacc
     9001 gctttgcaat ttaatattac tattagcggt agcagcgcct gatttcagtt ggatcaaacc
     9061 ttcatcattt ttatagatat tatttctatc cagcgtaact aacttaatcc tgtctaagat
     9121 tgccagagat ttgacatcgc cttctaaagg gacaatcgat atagttccgt ccgaaccaat
     9181 ctctatggat tgagcaggcg ggatagaaat ggggcctcca tcgcccaaaa caaccttacc
     9241 cgaggcagta attaactgtc cattagcatt caaacgcaaa ctaccccctc tggtataagc
     9301 ttcacgacct tggctgtcct gaacagccat ccacccgtcc ccatcaattg ctacatccaa
     9361 gtctctgcct gtcgtaataa tttccccagg agaagtgtcc actccattgg cattttgagt
     9421 tataaatgaa tgcatattga actgattatt tccatttgca ttattcatgt acatggtctg
     9481 tgcctggtac aaatcggcct taaatccagg ggtgttaata tttgccaaat tattggcgat
     9541 catttcctgc cttttaaaat ctgaacgacc gccagaaaca gccttataca ggataggctc
     9601 catagcttac ctcctagcga atattgatga tcgtttgagt aatcgcatca ccggttcgaa
     9661 ttgtttgagc attcgcctga aaattacgct gagcactaat taacttgacc aattcaccag
     9721 tcaaatccac attcgattct tcaagtcttc ctgaattaat taaccccaaa ccgccactcc
     9781 caggagcgct aaccaatggc tgtcctgaag aagtagtttc agtccagctg gtattgccta
     9841 tattttgcaa actttctgga tctgcaaaat tagccagctg aatctggccc attgctaaag
     9901 attgaccatt cgtataacgg ccatagatca ttcctgaatc atcaatatcc agtccagcaa
     9961 ggcttccggt agtgtagcca ttattataag ccgactgaac agcaaaggga ctgccaaatt
    10021 gtgtactatc cgataaatca atatttagat ttaaaggact ggaaccattg tttaagttga
    10081 atgacaaagg aattaaatta ttaggcaagg cagcgtttga ggtatcaaat actcctgcaa
    10141 acgagccatc agaattaaag ttagctcgga ataaattatt tgtattttga gctcccacat
    10201 taggaggaac gttttgatta tctatctgga atgctacata ccattgattt tctgttccag
    10261 caggtgatgc agcattaggt gaaccagcca aggcagtagg atccgcatga ataaaataca
    10321 tgctcaatac atgtgaattt cctaaactgt cgtagattgt tgacgaggtg acattgttat
    10381 aagtatcggt tgccggacta gcccctccga cccaatccac attcggcgct ttgctttgag
    10441 aattcatatt aacgcctact gagacatttg aactggctct gggggtaata ttcgaagtgt
    10501 ttattcttaa atcgcccgat gctcctgtaa ttgccccatt agaatcagtt agtaaaccag
    10561 tcaaccgttg attatttgca ttgactatat agttctcatt attcaacttg aattgcccgg
    10621 ctcgagtata aaccttggct ccttgatcac ttaatataaa gaagcccgaa ccattcacag
    10681 ccagatcgag tgtgttgttt gaagtactta atgttcctgt agcgaatgac tgatgtacac
    10741 gcgagagttt aacaccacca cctacagcat tgccgccaga gccataagcg ctggatgaat
    10801 aaatatctgc aaactcggct cttgaacctt taaatcctat tgtggcagag tttgcaatat
    10861 tattacctat aacttccaaa tctctggttg atgcctgaat tccacttaat gctgtattaa
    10921 aaaccataat agcctcctgc tttataattt tttagcattt gcctttgact gctttataag
    10981 caatgcgctt gtgacttaag ccatcgtttc aataaggcca cggtgactga aatcattgat
    11041 ttatactgat atttgcctta cctgacttaa atagactgat cctacgcccg ctacattaag
    11101 ctttagatcc tcgccatttt gcccaagact gacactatcg acattggctg acgtcatagt
    11161 tttcaatgct acttcttgtc cgccataagt accagtaacc tttattgtat atcttcctga
    11221 tgctaaacgt tgattacttt ggtcagtacc atcccaacta aattggaaaa acccgggttc
    11281 tggttgcccc aagggaatag tttttatcaa ctcgccagat tccgtgtaga ttgcagcacg
    11341 aagattagaa acaccaggtg ccatatcgat ggcagctttt acatatcctt ctgtacccag
    11401 actcagggta ttgctgttta ctaagacctt gcgaccaacc aaagcggatg cttgaagcgc
    11461 ttgattagat tgtagagatg aagctaattg ttgcagtgat tcttgcattt ttgtgattcc
    11521 atcattggtg ctaaactgag ccagttgaga taaaaactcg ccatttgcct gtggttgcag
    11581 aggatcttga ttttgaactt gtgccaccat caaacgcaaa aaatcctgtt gtcccaagtt
    11641 ttgtctttta tctgaatcaa tttgattcaa acctaaacca cctgctgaat tggaaccatt
    11701 tactgaattg gtagtcattc ccttcccctt attcgcctaa ttgcagcgtc ctttgcataa
    11761 gttgtttggt tgtattaagt aattcaatat tcatttgata agaacgagaa gctgaaatga
    11821 tatttgccat ttcttcaacg taattgatat ttgacgtata gacaaacccg tctttatcag
    11881 ctattggact atttggatcg taccttctgt gcggctctgc atcactctca taaataccat
    11941 ccagtttcac ccctgcctta atttgctctc ccatccattg atttgcattc tcctggactg
    12001 acttgaaaac aggatatttg gcttgataga cctcatctgg actacctgct tcaacattcg
    12061 cattactcat attgccagca ctggtagtca atcgcactgt ttctgcagtc attgcagaag
    12121 ccgcaatgtc aaaaattgca tttaatgaca tgattattcc cctttcaatg cggtcagcat
    12181 ggacttgatt ttgccattta agaaactcaa gctggcctga taattcaatg catttcgagc
    12241 aaactcggta gtttccatat ctttatcaac ggtattacca tctaaagaag catgatgtgt
    12301 cattcggtat ttcaaatgag tcataaaatc ggcattggta ttaatatgct ggtccgaagt
    12361 tacccttaac tgctggctgt taccggccat agttgctgtc agtacttcat taaaatcaac
    12421 atcccgggct ttaaaattcg gtgtattaac attggctatg ttattagcaa gaactgatgc
    12481 cctctgatcc cttgcaatca acgccttggc atgaatccca aaataagaat caagattaat
    12541 ggccatattt actcctgtct tattgttatt taagtcttaa tcatcatttg acattatgcc
    12601 aattttccaa agcactatat ggattcatac ttgtttccaa acttcactga aacataaaaa
    12661 tgataaaaaa atcctattta aacaaagcaa acattgtgcc aatatccaaa aaatgagaaa
    12721 atgaagcatc caagcgctaa ttccctatgc ggagtctcat catgtcaatc tcgaagacac
    12781 ttccttataa tgcgatagag caaattaaat cttatctgct tcaactccaa aatacaattt
    12841 gtgtctcact ggagtcaatt gatggcaaaa ccagattcca tgaggattcc tggcagcgag
    12901 ctgctggagg tggaggaaaa acaagaataa tggcgaatgg gaatgtcttt gaaaaagccg
    12961 gcgtcaattt ctcacatgtt tcaggggaac aattaccagc atcagccagt gcacatagag
    13021 aagaattggc aggaaggcat ttcagcgcat taggtgtatc tctggtcatt catcctcaaa
    13081 acccctatgt gccaacaaca catgccaatg ttcgattttt tgtcgctgaa aaggaagatt
    13141 cggaaccagt ctggtggttt ggtggaggat ttgatttaac tccttattat ggatttgttg
    13201 aagactgtga gcattggcat caaacggcat taaatgcctg tttacctttt ggagaaacga
    13261 tttacccaaa attcaagcgt tggtgcgatg attatttttt tattaagcac agaaatgaag
    13321 cacgtggaat tggcggttta ttttttgatg attacaatga aatcagcttt gatcacagct
    13381 ttgaactcat gcgtagcata ggcgatcatt ttattctggc ttatgaaccc atagtggctc
    13441 gccgtaaaga catccctttt ggcaacaggg aaaaagcatt tcagaattac cgcagaggcc
    13501 gttatgcgga gttcaattta gtctatgatc gaggcacttt atttggtctg caatctggcg
    13561 gaaggactga gtctatattg atgtccctgc ctcccattgt tcattgggaa tacaattggc
    13621 atcctgaaaa aggcagtgat gaagaaaagt tatacacaga ttttttacct gcaaaagatt
    13681 ggttgaaaaa ggagtaaaac accaaactca aaaataaatg atggctttct ccccttacct
    13741 gaaaatttcg aggagccagt atacatgcag taaatgagca ccgcgaaacg tttacaacgc
    13801 gcaataaaat gaaagagaca ggttatgcaa gagatctgtt gttaagctat ctcctctgag
    13861 cttcttctta aggttcttaa cccaagaaac aagaacaccg cggcaaccaa atttaaaaga
    13921 caaagtatca aaaaaacctg aggaattgaa aagctaaaaa acaataacag catgactaac
    13981 aaagttccca tcaccataaa caaggcattg aaaatattgt tcgccgcaat cgttctagcc
    14041 ctgttctcaa ctggacttat aatttgcaag taagtataca agggaactac aaatagtcct
    14101 gaacaaaatg ctaacataaa taaatcaaaa gcaattcgcc aatgagtaaa tgaagtaaaa
    14161 aaattaaata agttaaataa gggggtcttt aaatcatgac ccgcaggaga tgaccaatac
    14221 aaatcaaaag caaaaaaagt aaaagcgagc atggccattg ggacagcaga aagattaact
    14281 tgtcctttca ataaccaatt tatagtaatt gatcccagag caatgcctat tgaaaataaa
    14341 gcaagaaata gagcaaaaac atgagcgcta gcccccaaaa cataatgggt ataatcaggt
    14401 aatttggtaa gcatgacagc gccaattaac caaaaccagg aaatagttaa tatggatata
    14461 agaattccat attctttttt cgcctccttt aacatcttca ctgtggcccg ccataaatta
    14521 aaatccactt ttaattcatg aagtgctgat ggtgcaggag gtataaacag gctggagacc
    14581 aaaccagaaa aagcggcact taaggtcata ataatcgcga tataagggat agcctccttc
    14641 cctccaacgg ttaacgtacc caatgtagtg cctaataaaa tagcaataaa agtgctggct
    14701 tcaattaggc ctgtcgctgc taataaattt ttcttaggca aatggtctgg taaaatcgca
    14761 tatttgatag gtccaaagaa agaagaatgt acccccatcc cagtcaaagt acacatcatc
    14821 aagaaaatgc taccactgta taatgcaata cctccaataa tcattaataa cacttcaagc
    14881 accttaacca gacgagtcaa caaagccttg tcatatttat ctgccaattg tccggaagta
    14941 gcagaaaaga taaagaaagg caggatgaat aaagcgcctg caatagcctg ataatattcc
    15001 gactgctctt gtgtatggct taaatgatag ctaatcatag tcaacatcgc tagcttataa
    15061 gcattatcgt taaaagcacc aaaaaattgg gtcaggaaaa ggggcaaaaa ttttctttgt
    15121 ttgagaagac caataaaacc cgctgtcatt aaggctccat ttgtgcttta aaaataaagt
    15181 aaacaaggat attggtgtat tgaacgaaag taaatgataa ataccagata aaaagcttat
    15241 aggatgccac tcccactatt taatttacaa atgcagcaca ttgtgattgc tatttcgcga
    15301 tattaatttt ggctgaattt atttcaggaa ttggaatgac aaaagaacat acaggagatt
    15361 tacattagcc aataccccaa tcggctcatc cttttttact tatcatttga aaaataaaat
    15421 tagcccggtc ttttataggg catttgggaa cctctaataa cacataccca caatcaaaat
    15481 acccctcttt tacagcataa taagtttgca acgcttcttg ataatcctgt tggcgctcat
    15541 cgtcattttc ataaatttca ggccagggag ggaaaataaa ggctgtttta ttataacgaa
    15601 atcgcctaac agcctcattc actttcttac tcgcctcacc acaaaatgct ttggagtaac
    15661 catacaaatc cgggattccc cgatcgaaaa atacaatttt ttcctctgac tgcatcttgc
    15721 aataatctgc aatcgattgt tcaagcatca agtctcgaaa cgcttttcta tttccagtat
    15781 gtatcgcatc accaccatca gcctgttgtt tttgaataat agaacgtgct acttcagaaa
    15841 ctgttaaata gccccaagta tttaactcat tcaatacgga agttttgcca gagccaggcc
    15901 ctcccgtaaa tataaaaaaa ttagtgcata gcattaaatc cgctaccata acctgtcata
    15961 cctttaaaaa ttgctcataa attagattac aaagctttta ttgtactcat atcaattctc
    16021 ttaagctttt atccagctta tctgtttcgc aataataaaa atgggtatcc aaagggcata
    16081 gctcaaattc tttaggtaaa gaattcttat taacaagaac aaatccgttc ttttcataaa
    16141 aacgtcttgc tgcgtttaac tgagctactg ttcccaaaaa cagtttttta aatccatgtt
    16201 tcatggcgtt tctaactaaa gtcagcatta atttttgagc taatcctaac cctcgatact
    16261 tcggcgcaat aaaaaatttt tttacttccc cttcagtatg atttaacttc tttaaaccga
    16321 tagaaccgat aatccccccc tgttcgtcaa ccgcatacca aaaattacaa ctgctataat
    16381 agcaatactc ctcttcggcc tttaaaattg atgcattgat ttcaggagta atcgaaagat
    16441 gaaactcatt cacttgaata ttttcaatca tattaacaat ttgtttacgc aaagctctgg
    16501 aagttgacaa gcgtaaaatt ctaatctgat cacgaattaa acgactttgt tctaaagctt
    16561 gagcatattt tccaattgca gtaattactt gttctttctc ttcgtcagtt aaataatgaa
    16621 atgctctttt tattttagta ttagaatatt catcaatata atttaccttt tccttaccct
    16681 tatcagtgac tcttaaaaag cgctctctct tatccagccc ttcgtttacc gtaaccaatt
    16741 catcattaat caaagaattc actattcgcg
//
LOCUS       DATNSN010000064        16731 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.064, whole genome shotgun sequence.
ACCESSION   DATNSN010000064 DATNSN010000000
VERSION     DATNSN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16731
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.064"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..250)
                     /locus_tag="VAH76_001665"
     CDS             complement(<1..250)
                     /locus_tag="VAH76_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HEO1386292.1"
                     /translation="MILNIRRMKDSDIENVYSIETNVHIAPWSKDILRDCVLVGYDCR
                     VLEINNGDSSILAGYIISRISNNSCHILNLCIAKPLQSK"
     gene            complement(585..2009)
                     /gene="lpdA"
                     /locus_tag="VAH76_001666"
     CDS             complement(585..2009)
                     /gene="lpdA"
                     /locus_tag="VAH76_001666"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO1386293.1"
                     /translation="MANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGV
                     CLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLK
                     ALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKR
                     IFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLV
                     NILQKRMTKKGVKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAVGRKPN
                     GGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEV
                     IAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGR
                     EEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSE
                     TIAQSAEAFEGTITDLYLPKKKKK"
     gene            complement(2098..3732)
                     /gene="aceF"
                     /locus_tag="VAH76_001667"
     CDS             complement(2098..3732)
                     /gene="aceF"
                     /locus_tag="VAH76_001667"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEO1386294.1"
                     /translation="MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEK
                     ASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKAESEKQNSQT
                     RAEEQSVDTKATASTPGSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
                     DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKASAKSETPEIEKSQIKNISEQS
                     IKEIEKPYEELKSEPISINNLEIAESKSIIISAGPAVRRLAREFGVDLSLVQGSGRKS
                     RVTKEDLQNYIKVRLNEKTTSGGFSLPSNPAIDFSKFGSIETKPLNKIKKLTGANVHR
                     SWITIPHVTQFDEADITDLEAFRKSESESTKNQDYKLTLLAFVCSVVCKALHAYPQFN
                     ASLDASGENLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREK
                     GLTPIDMSGGCFTISSLGGIGGTAFTPIVNSPEVAILGLSRSVIKPIYDNKEFKPRLM
                     LPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL"
     gene            complement(3790..6453)
                     /gene="aceE"
                     /locus_tag="VAH76_001668"
     CDS             complement(3790..6453)
                     /gene="aceE"
                     /locus_tag="VAH76_001668"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEO1386295.1"
                     /translation="MTNETNLDLDPIETREWLDALQAVLFNDGPERTAFLLQQLLNKA
                     NAEGVKLTSSINTPYRNTIKPHEEKQMPPDEGIGKRINALIRWNAVAMVLRAGKYAPE
                     LGGHIASYASASTLYETGFNYFFKGQKGENGGDLLYIQGHSAPGIYARAFLEGRLTEK
                     QLEKFRQEVEVDGLSSYPHPWLMNDFWQFPTVSMGLGPLQAIYQARFLKYLENRGLIK
                     AEGRKVWAFLGDGEMDEPESVGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQ
                     ELEGLFRGAGWNVIKVIWGGRWDPLFARDNQGWLQKRMEECLDGDYQSYKANDGSYVR
                     QHFFNQYPELKKMVENMSDEEIWRLNRGGHDPQKVYAAYARAVEHKGTPTVILAKTIK
                     GYGMGAAGEGQNITHQQKKMTIDQLKAFRDRFNIPISDDKIADIPFYKPDDDSPEIKY
                     IKKQREALGGYLPHRSTEVEQLKIPDLGEFSSITKGLGDREISTTMAFVRILSALLKN
                     KDINSRIVPIVPDECRTFGMEGLFRQIGIYSPVGQLYTPVDHEQVMYYREAVDGQILE
                     EGINEAGAFCSWIAAATSYSSNKLAMIPFYIYYSMFGFQRIGDLAWAAGDMQARGFLL
                     GGTAGRTTLAGEGLQHQDGHSHVLASTIPNCISYDPTYAYELAVIIQNGLHRMYEKQE
                     NVFYYITIMNENYSHPDMPAGVEEGIIKGMYLLKENKKKSKNHVQLMGCGTILREVIK
                     AAEMLEEDFSITSDIWSVTSFNELRKEGLAVERYNNMHPQNKPQESYVTSQLKGRRGP
                     VIASTDYMRIYADQIRPFVPNRYITLGTDGYGRSDTRTQLRHFFEVDAKFIVLAALNA
                     LVAEGSLDKAKIVDAMKRYNINQDKLDPMTH"
     gene            complement(6481..6870)
                     /locus_tag="VAH76_001669"
     CDS             complement(6481..6870)
                     /locus_tag="VAH76_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1386296.1"
                     /translation="MNTAFRLLFCLIILELSACATLKSKITHHKTLSQCQQTCFQQLN
                     YCKQNCTDNCRDCSTKVDHFAKENYLEYLHEVKIQGGYITRGLQSYRDPLQCRKVTCN
                     CSADFNACNQGCSGVIQKRLQPVPYCS"
     gene            complement(6909..7661)
                     /locus_tag="VAH76_001670"
     CDS             complement(6909..7661)
                     /locus_tag="VAH76_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386297.1"
                     /translation="MKLLVIVLCLLSERFLIHSVSYQRFSWFNNYCLFLKKFIDKNEY
                     FSNPWATLIAIILPIVFLTFLIYFSLQSILFGLFGLILSLFIFYYCLGPQNAFYPILK
                     KQANQTETDAIGEYFAEVNSQLFAVVFWYIIAGPIAALTYRLIALCKEINFISTQASQ
                     ITSILEWIPARITALLFLLVGNFQRGFHLFVQYVLTSPDSNDKILRGCGLQAVRINDT
                     EEVPMAAAENLVEHATIVLLVFIALFTLVAWL"
     gene            complement(7666..8838)
                     /locus_tag="VAH76_001671"
     CDS             complement(7666..8838)
                     /locus_tag="VAH76_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1386298.1"
                     /translation="MHDGSVFYTIFLIFAGAAFFSTLVLYTKQSLLVAYITLGAILGP
                     WGFKLVSDVTVVKQIGDIGIVFLLFLLGLHLQPQNLIHMLRKVTWIAIVSSILFAALA
                     YGIGRWFGLTVTESLILGGAMMFSSTIIGLKLLPTTILHHQHTGEVMISVLLMQDVIA
                     IILLILLNSAHQDGNLSINDFILVIIALPTLCLFSFLFERYILIKLLARFDRTQEYVF
                     LLSIGWCLGISVLAQTIGLSEDIGAFIAGVALASSPISLFIAESLKPLRDFFLVMFFF
                     SIGATFNFSFGAQVVIPAVILATLILIIKPLLFYILLARSGEKKQVAKEVGIRLGQGS
                     EFSLLVASIALSANLISEVASNLIQATTILTFIVSSYLVVLKYPTPIALSEKMRKD"
     gene            complement(9156..9977)
                     /locus_tag="VAH76_001672"
     CDS             complement(9156..9977)
                     /locus_tag="VAH76_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEO1386299.1"
                     /translation="MRKLLIVFSIFLASCQATHDKPNLTNKKRQTTHTKNSVYDRYKY
                     TSNRYTIQQDGAPKQAKQVSFKEPVPVKEPLSRYGNPSEYYVDGRTYEVMKSSTGYKT
                     RGIASWYGTKFHKQRTSSGEPYNMYVMTAAHKTLPLPTYVKVRNLENGKVAIVKVNDR
                     GPFHANRVIDLSYAAALKLGVFPKGTAHVEIETLMGPAGQGHYYLQAGAFSSEALAKR
                     LKEKLIHITPSPVFIEHYKQHYIVRVGPFGNKSMADNLKTKLSNNGIKGSFSVLI"
     gene            10173..11396
                     /locus_tag="VAH76_001673"
     CDS             10173..11396
                     /locus_tag="VAH76_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO1386300.1"
                     /translation="MTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTT
                     KPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQ
                     IHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLGGSE
                     NAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
                     QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEP
                     SDSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIP
                     AGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTR
                     TKDSIRLAFKRWFGS"
     gene            11409..12245
                     /locus_tag="VAH76_001674"
     CDS             11409..12245
                     /locus_tag="VAH76_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEO1386301.1"
                     /translation="MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYF
                     VDQHLNRLFSNMKKIKMTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGVRKHDI
                     PTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
                     SAGFHTSILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLD
                     LKFREIEISISELFSAQEVWVTSTTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQL
                     VN"
     gene            12249..12512
                     /locus_tag="VAH76_001675"
     CDS             12249..12512
                     /locus_tag="VAH76_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEO1386302.1"
                     /translation="MTKTTLIEFPCYFPIKIIGINSELLLNEIRQIVINHFPTFKNEH
                     LTHKTSKQDKYLAITVTVYAENQISLDGLYQDLSQHPAVKMVL"
     gene            12512..13111
                     /gene="lipB"
                     /locus_tag="VAH76_001676"
     CDS             12512..13111
                     /gene="lipB"
                     /locus_tag="VAH76_001676"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEO1386303.1"
                     /translation="MIIHNLGIKDYTEIWEQMKAFTAIRDSNSCDELWLLEHYPVYTQ
                     GQAGKPEHVLNPNSIKIVQSDRGGQVTYHGPGQLVAYVLMDIRRRNLGIRTLVAKLEE
                     ILISVLKHYKIPANIRSGAPGVYVGEKKIASIGLRVKNGCTYHGIALNVNMDLSPFLG
                     INPCGFAKMEMTQMSHFHPNIQLEEVSQHFVQYFLTQFK"
     gene            13121..15157
                     /locus_tag="VAH76_001677"
     CDS             13121..15157
                     /locus_tag="VAH76_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO1386304.1"
                     /translation="MNLLADYVQPLTYWLQANPHWSLFITFLIALSESLAVIGSIIPG
                     SVTMTAIGILAGSGIMRIDLTLLAATLGAICGDSLSYFLGYFYSDRLIEIWPFRKYPG
                     LLKYGKDFFASHGGKSVLIGRFVGPLRSIIPVIAGIMHMKQWRFLIANIVSAIGWSIL
                     YVMPGVLIGAAGHELSAEGATRFFILILVILAVIWLSSLVLKWLFIKLNSFLRNNLHR
                     FWLEIKTSPGLSKVYYAITPVQEENHYNTVGLLLFALVIFFLFLILVIFTIEGQLLDY
                     INVPVHFFMQSFHSSLLEAVFIVCAQLTSKSTLAGLFIASCIWFIYHKNYRATVYLSS
                     ITLLSGVIALFLTKIIDSPRPQGLLITLPGSSFPSVSVSIATALYGFILFYINSKYSL
                     ITSSFKTVILIVLGLSGFANIYLGDFWFSDILAAYLLGAEVCLIHCLVYRRFSTSAIK
                     NVHPLIMLISLLMVIVFATAVSTVVNFKELVHDHMPYHKEFTLTEKQWWDQQRPLLPL
                     YRLSRIGKRISLLNIQYVGDLDALQNKLEQFGWEPQSDKTFFTRLLMRMNSAPSNVKL
                     PLLTQLYENKKPGLIMTYQDKKSKLIFDLTIWQSNYYLSEPKRMVWIGSIHQNIFNLK
                     QKKSKHNLPPVLNPLSYVIPALDDFTLRRIALTDDLVKTSVFPTAPFILLIKTK"
     gene            15283..15888
                     /locus_tag="VAH76_001678"
     CDS             15283..15888
                     /locus_tag="VAH76_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system protein LssZ"
                     /protein_id="HEO1386305.1"
                     /translation="MHILAKVIHCIFPLIALILLIIGIKRNAIYYIISSLWVSLISLV
                     IHYQTSGGEILGTYFNYINATIYTINLFVLCLSLVYVLWHLSNDNVAFKIVSSLFQSF
                     IVIGALLVVINLWINAYFIENRLTGTPVMQVALLEKPEYCDYRYIFYKVALDGTVHYL
                     CPNHYGLIPSIGRLSISPDFIASQLSIPSKKQMLLLQQKRS"
     gene            15973..16674
                     /locus_tag="VAH76_001679"
     CDS             15973..16674
                     /locus_tag="VAH76_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386306.1"
                     /translation="MKRFFSILFILYCCNLFAMAPKLNWDKAIDKAIKRYGLRVEPQL
                     ISYFSKAGVSYPPKEIALLAFKSERKVELWAKNPSQEWTHIHDYPLTGFSGRLGPKLR
                     ENDKQIPEGIYKLVNFNPFSSMHLSMMINYPNSFDRQKGYMDGRKELGNNIFIHGKNL
                     SVGCLAVGDLAIDQLFILARRVGLKNIQVIIAPNDLRYQKPSTSTFAQPRWLPELYKQ
                     ITESLKPFKKTKFTA"
BASE COUNT         5043 a         3118 c         3034 g         5536 t
ORIGIN      
        1 cttttgattg taatggtttg gcaatgcaca aatttaatat atgacaacta ttattgctta
       61 ttctggatat gatatatcca gcaaggatcg agctatctcc attgttgatt tccagaactc
      121 tgcaatcata accaacaagc acgcaatctc ttagtatgtc tttactccag ggggcaatat
      181 gaacatttgt ttcgatagaa tagacatttt caatatcact atctttcatt ctacgaatgt
      241 ttaatatcat tcattactcc tgggaataat catacttaat ttatgatgaa ttaaatatta
      301 gcaaatgtgt tatcaaacat tactaatcca aaatactgca cttaataatt ctttagtttt
      361 ttatttagac cctttgcatg accgtccttt cattttttgc tgtactgcag atatttttgt
      421 gggatgtatg taaactctac tccgcaaaaa gtttgtatct agcccaaaat aaaataatca
      481 gcccatgcaa gaggtctata tataattata tacatataat aagcatccct ttgcttgtgt
      541 gagcaaagga aatgcaatga ggtataattg gctacctgta attattattt tttcttcttt
      601 ttgggcaaat acagatccgt tatagtgcct tcaaatgctt ctgctgactg agctatggtt
      661 tctgatagag tcgggtgagg gtgaatagta agagcgatgt cttcaacatc acaacacatt
      721 tcaatggcta aggcagtttc tgcaattaaa tcgccggcat taaccccaac aatacctgct
      781 cctaggatac ggttagtttc tgggcagaat agtaatttag tcatcccctc ttctcgcccc
      841 atgctgagtg cacgaccgct agcagcccat ggaaaggagg ctttctcata gtttattcct
      901 ttctcttttg cttctttttc tgttagacca gcccaagcta attctggatc tgtataagca
      961 acactaggta tacattttgg ctcaaaataa tgttttttcc ctgcaatgac ttctgctgca
     1021 actttccctt cagggatggc tttatgtgcg agcattggtt gtccattgac atcgccaata
     1081 gcaaaaatat gaggaacatt cgttcttaat tgattatcaa ctggaataaa gccacgttca
     1141 tctactttga tccctgcttt ttcagcgttg attgccccac cgttaggttt tctgcctact
     1201 gcaacaagta cttgctgaaa acaaagtggc ttatcagttg catgttctcc ttccattgaa
     1261 acataaatac catctttttt ggcttcgact gcagttactt tcgtttttaa tagaaatttg
     1321 acaccctttt tagtcattcg tttttgcagt atattgacca ggtcagcatc agcattcgga
     1381 attaattggt ccataaattc aacaactgtt acattaactc ccagggagga gtagacagtt
     1441 gccatttcca agccaataat accaccaccc aatacaagta agtcaccttt gatatcagcg
     1501 agttctaatg ctcctgtaga gctaaatatt cgtttatctt ctggtataaa tggtaattta
     1561 atggattctg agcctactgc aataatggca ttatcaaatt caatttctac agtcccttct
     1621 ttcgtttcaa caagaatttg atgggtacct gaaaacttac ctgtcccagt aattacttct
     1681 acttttcttt gtttggcaag agcttttagt cccccggtca actttgcaac gacagaattt
     1741 ttccaggcaa ccaatttttt attgtcgaat ttgggtttac caaatgctac accttgctcc
     1801 gacatttcat gggcttcatc tactacttta gcaatatgca ataatgcttt tgagggaatg
     1861 caaccaacat ttaaacaaac cccacctagt gagtcaaaac gctcgaccaa aaccaccttt
     1921 ttatccaagt cggcagccct gaaagctgcg gtgtatccgc ctggtccact acctaatacc
     1981 actacatctg tcttaatttt attagccata agaagcctca ttaaaaaata tgttttctgg
     2041 ggttattggc atttcattca gaacaaggaa ataccaatag ctctattcct ttatatgtca
     2101 gagtaaaact cttctaatat cacctaaaca atcacaaaga aagcgagtaa accgggctgc
     2161 ctcagcgcca tcgattactc tatgatcgta agaaagggaa ataggcaaca tcaatctggg
     2221 cttaaactct ttattatcgt atatgggttt tattactgat cttgataagc ccaaaattgc
     2281 aacttccggg ctatttacaa taggagtaaa tgccgttccg cctataccgc caaggctgga
     2341 aattgtaaaa caaccgcctg acatatcaat aggtgttaat cccttttctc tagctttcgt
     2401 actgagtctg gacatttcct ttgcaatatc aatcacactc aatttatcaa cattctttat
     2461 tacaggaact accagaccgt taggtgtatc tactgctata cctatattat aatatttttt
     2521 ataaattaaa ttttcaccac tggcatccag tgacgcatta aattgcgggt aagcatggag
     2581 agccttacaa actacgctac aaacaaatgc taacagagtt aatttgtaat cttgattttt
     2641 agtgctctct gattctgatt ttctaaacgc ttccaggtct gtaatatctg cttcgtcaaa
     2701 ctgagtgaca tgtggaatgg ttatccaaga gcgatgtacg tttgctccag taagcttttt
     2761 aattttattt aaaggctttg tttcaattga accaaattta ctgaaatcaa tggctggatt
     2821 tgacggtaag ctgaagccac cagacgtagt tttttcattt aaacgtactt tgatataatt
     2881 ttgcaggtct tcttttgtaa cacgagattt tctcccactt ccctgaacca aagataaatc
     2941 gactccaaac tctcttgcca accttcttac ggctggacct gcagatatta tgatactttt
     3001 tgattcagct atctccaggt tattaattga tattggttcg gattttagtt cttcatatgg
     3061 cttttctatt tcctttatag attgttctga gatattttta atctggcttt tttcgatttc
     3121 aggtgtctca gattttgctg aggcttttag agttagaata ggagtacctt gagatacttt
     3181 atcccctaat ttaattttca tttcgatgac tttacctgca tagggcgaag gaatatccat
     3241 cgttgcttta tcaccctcta atgtaattaa tgcctgatcc ttttctactt ccatccctgg
     3301 tttgactaaa atatcaatta catcaacgtc atttgcacca ccaatatccg ggatagatat
     3361 ttcaatatct ttggagccag gtgtagatgc agttgccttt gtatcaacag attgctcttc
     3421 tgctctggtt tgcgaattct gcttttctga ttcagccttt tgttcttgag aactggaaat
     3481 atttgtcgtt gtatctgact tggctttgac aatgagatcg ccttcggata ctttgtcacc
     3541 gactttaacg agaatttgcg ttaccgttcc agatattgga gaggggatgt ccatgcttgc
     3601 cttctcagac tcaagcgtaa tcaatggtgt atccacttcg atctgatcac cctcttttac
     3661 taaaatctca atgacatcaa cttgattagc gccaccgata tcaggaattt taatttcact
     3721 ttcttttgtc atattttcct cagcactcag ttaaaccgga actatcttct gttctgatta
     3781 attgtttaac taatgggtca ttggatccaa tttgtcttga ttaatattgt aacgtttcat
     3841 ggcatcgaca atttttgctt tgtcaaggct accttcagct acaagtgcat ttaatgctgc
     3901 caacactata aattttgcat caacttcaaa gaaatgacgt agttgtgtac gggtatcact
     3961 tctgccgtaa ccgtctgtac ctagggtaat atagcgatta ggaacaaaag gcctgatctg
     4021 atctgcataa atacgcatat agtcagttga ggctataaca ggtccacgac gtccttttaa
     4081 ttgagatgtc acataacttt cctgaggctt attttggggg tgcatgttat tgtatcgctc
     4141 tacagccaaa ccttctttac gtagctcatt gaagctggtt acactccaga tatccgatgt
     4201 aattgaaaaa tcctcttcca gcatctctgc ggctttgata acttcacgca aaatagtacc
     4261 gcatcccatt agttggacat ggttttttga ttttttctta ttttctttta agagatacat
     4321 tcctttgata ataccttcct ctacccctgc aggcatatcg ggatgagagt agttttcatt
     4381 cataatagta atgtaataaa acacgttttc ctgtttttca tacatacggt gcaatccatt
     4441 ttggatgata actgctaatt cataggcgta tgtgggatca taagaaatac aattaggtat
     4501 tgttgatgct aaaacatgac tatgtccatc ttggtgttgc aatccttctc ctgccagcgt
     4561 tgttcgtcca gcggttccac cgagcagaaa tcctctcgct tgcatgtctc ctgccgccca
     4621 tgctaaatca ccaatgcgtt ggaaaccaaa catagagtaa taaatgtaaa atggaatcat
     4681 tgccagctta tttgaactgt aagaggttgc ggcagcaatc catgaacaaa acgccccagc
     4741 ttcattaata ccttcttcaa gaatctgccc gtcaactgct tctcggtaat acatcacttg
     4801 ttcatgatct acaggagtat aaagttgacc tacgggagaa taaattccaa tttgtctgaa
     4861 taatccctcc ataccgaatg ttctgcattc atctggtact atgggaacta ttctggaatt
     4921 aatgtcttta tttttgagta gagctgaaag aattcgcaca aatgccatag tagtcgagat
     4981 ttctctatct cctaatccct tggtaatact ggaaaactct cctaaatcag gtatttttaa
     5041 ctgttctact tcagttgaac ggtggggcaa ataaccgccc aaggcttccc tttgcttttt
     5101 tatatacttg atttcaggac tgtcgtcatc aggtttatag aaaggaatat cagcaatttt
     5161 gtcatcgctg attggaatat taaatcgatc tctgaaagct tttagttgat ctatggtcat
     5221 ttttttctgt tgatgagtaa tgttttgtcc ctcaccagcc gcacccatcc cgtagccctt
     5281 gatggttttt gcaaggataa ctgttggagt tcccttgtgt tcaactgctc tggcataagc
     5341 tgcatacact ttttgaggat catggccacc acgatttaat cgccaaattt cctcatcaga
     5401 catattttca accattttct ttaattctgg atattgatta aagaaatgtt gtcttacata
     5461 cgatccatcg ttcgctttgt aagattgata atctccatcc agacattctt ccattctttt
     5521 ctgtagccag ccttgattat cacgggcaaa taatggatcc caacggcctc cccagatgac
     5581 cttaataacg ttccatccag ctcctcgaaa taggccttca agttcttgaa tgattttacc
     5641 gttaccacgc acaggcccat caagtctttg taaattgcaa ttaactacaa aaatgagatt
     5701 atccagcttt tctcgtgctg cgatactgag tgccccgact gattcaggct catccatctc
     5761 gccatcaccc aaaaatgccc atactttcct accctcagct ttgatcagtc cccggttctc
     5821 aagatatttt agaaagcgag cttgatatat tgcttgtaga gggcccaaac ccatggatac
     5881 ggtgggaaat tgccaaaaat cgttcattag ccaagggtgt gggtaggaag ataaaccatc
     5941 tacctctact tcttgccgga atttttcaag ttgtttctct gttaatcggc cttcaagaaa
     6001 tgctctggca taaatgcctg gagccgagtg gccttgaata taaagcaagt cccctccgtt
     6061 ttcgcctttt tgacccttaa aaaaatagtt aaatccagtt tcatataacg ttgatgcaga
     6121 tgcataagaa gcgatatggc cgccaagctc tggcgcgtat ttacctgccc taagtaccat
     6181 tgcaacagcg ttccatctaa ttaatgcatt gatgcgcttt ccaattccct catcgggagg
     6241 catttgtttt tcttcgtgag gttttatagt gtttctgtag ggagtattaa ttgagctggt
     6301 tagtttaacc ccctcggcgt tcgctttatt aagaagctgc tgtaaaagga atgcagtacg
     6361 ctcaggacca tcattaaaga gtacagcttg tagggcatcc agccattcac gcgtttctat
     6421 tggatccaga tctaaatttg tttcattggt catgaaatag ttccccaaca atcaataatt
     6481 ttatgaacag tatggtacgg gttgtaatct tttctgaatt acaccagaac aaccttgatt
     6541 acatgcatta aaatccgcag agcaattaca agttactttg cggcattgta gtggatcacg
     6601 gtaagattgc aacccgcgag taatatatcc accttggatt ttgacttcgt gaaggtattc
     6661 cagataattt tctttggcga aatggtctac tttcgtagaa caatctcggc agttgtctgt
     6721 gcaattttgt ttgcagtaat taagctgctg aaaacaagtt tgttggcatt ggcttaaggt
     6781 tttatgatgg gtaattttac tttttagtgt ggcacaagct gaaagctcca atattataag
     6841 gcaaaaaagt aaacggaatg cagtgttcat gattcaagtc ccacattaat ttattgtgaa
     6901 atagaacatt atagccaagc tactaaagta aataatgcaa taaaaaccaa caacactatt
     6961 gtggcatgtt ctacaagatt ttcagcagca gccattggta cttcttctgt atcattgata
     7021 cgaactgctt gcaaaccaca tcctctcagt attttatcat ttgagtcagg agaagttaga
     7081 acatattgaa cgaatagatg aaaacctcgt tgaaaatttc caactaacaa aaacagcaaa
     7141 gcggtgattc tggctggtat ccattcgaga atgctagtaa tttgacttgc ctgagtactt
     7201 ataaagttaa tttctttaca taaagcgatt aatctgtatg ttaaagctgc tattggccca
     7261 gcgatgatgt accagaatac aacagcaaat aattggctgt taacctcagc aaaatattct
     7321 ccaattgcat ctgtttcagt ttgattagcc tgtttcttca aaattgggta aaaagcattt
     7381 tgcggcccca gacagtagta aaaaataaac aggctaagaa ttaggccaaa taaaccaaac
     7441 agaatacttt gtagtgaaaa ataaataaga aatgtgagaa aaacaatggg caatatgata
     7501 gctatcaagg tagcccaagg attagaaaaa tattcatttt tatcgatgaa ttttttaaga
     7561 aataggcagt agttattaaa ccaggaaaat cgctgatagg acactgaatg gattaaaaac
     7621 cgttcactga gcaagcataa tactattacc agtaatttca tttgattaat ccttacgcat
     7681 tttttcagat aaagctatag gtgttggata tttcaataca acaagatacg atgaaacgat
     7741 aaaagttaaa atagtagttg cctggattaa attggaggcg acttctgaaa tcagatttgc
     7801 actcaaggcg atgctcgcaa ccaataaaga aaactcactc ccctgcccca agcgaattcc
     7861 tacttctttt gccacttgtt ttttctcacc agaacgggca agcaatatat aaaatagcaa
     7921 cggcttaata atcagtatca atgttgctaa tatcaccgcc gggataacta cctgagctcc
     7981 aaaactaaaa ttaaaagtag cacctatcga aaaaaagaac atgacaagga aaaaatctct
     8041 taatggcttt aaactttcag caataaacaa tgaaattggg cttgatgcca aagctactcc
     8101 tgctataaaa gcgccaatat cttctgataa gcctattgtt tgtgctaaaa cggatatccc
     8161 caaacaccat cctatggaga gtagaaaaac gtattcctgg gttctatcaa aacgtgccag
     8221 caattttatc aggatatatc tttcaaataa aaaagagaac aagcacaggg tcggtaacgc
     8281 gataataacc aggatgaaat cattgattga caggttccca tcctgatggg cactattaag
     8341 taatatcaaa agaattatgg ctataacgtc ctgcattagg agtacgctaa tcatgacttc
     8401 accagtatgc tggtgatgta aaatggttgt tggtaataat ttcaagccaa ttatagtact
     8461 tgaaaacatc atggctccgc ccaatatcaa tgactcagtc accgtgaggc caaaccatct
     8521 gccaattccg taggcaagtg cagcaaacag aattgagctt acgattgcaa tccaggttac
     8581 ttttcttagc atatgaatca aattctgggg ctgtaaatgc aaacctaata agaaaagcaa
     8641 gaatactatt ccgatgtcac ctatctgctt gacaaccgtc acatcggata caagtttgaa
     8701 tccccaggga cctaagatag caccaagtgt tatataagca accaataatg actgtttggt
     8761 gtacaatacc aaggtagaaa aaaaagcagc tccagcaaat atcagaaata tagtgtaaaa
     8821 tacagagcca tcatgcattg attgaatacc tcttaagaaa aggtagttaa aattgaatta
     8881 taaagccaat tttatccatt aatctattaa ttagctatta attattgttt attaataata
     8941 aattgttgcc tttagttaga gccaaggtga tatctattga tgagaatgtc ctcgattgag
     9001 tcataatgtg ttagtaaaat taacacaagc tatgcacaca ttcagaatca gtctgagtaa
     9061 agtgtttgac ctaaaactat ttctttccca aactattctg agtggatttt tttaatttaa
     9121 agatataagt taaccagttt tttccacgaa acaaattaaa ttaacacaga aaaggatccc
     9181 tttattccgt tatttgataa ttttgtttta aggttgtcag ccattgattt gttaccaaaa
     9241 ggacccactc ggacaatata atgctgttta tagtgttcaa taaacaccgg cgacggtgta
     9301 atgtgaatta gtttctcctt caaacgcttt gccagcgctt ctgaggaaaa ggctcctgcc
     9361 tgcaaataat aatgaccttg cccagcaggc cccattagtg tttcaatttc aacatgagca
     9421 gttcctttgg gaaaaactcc cagttttaat gcggcagcgt aagagagatc tatgacacga
     9481 ttcgcgtgaa atggccctct gtcattaacc ttaactattg caactttgcc attttccaag
     9541 tttcttactt tgacataagt tggcaaaggc aatgttttgt gggcagctgt catgacatac
     9601 atgttatagg gttctccact ggaggtacgc tgcttatgaa attttgttcc ataccatgag
     9661 gcaatcccac gagttttata ccccgttgag cttttcatga cttcataagt acgcccgtca
     9721 acataatatt cggaaggatt accatatctg cttaatggtt ctttgacagg cactggttct
     9781 ttaaagctta cttgtttggc ttgtttggga gctccatcct gctgtattgt ataacgattg
     9841 gatgtgtatt tatatcgatc gtagacagag tttttagtgt gagtggtttg gcgtttttta
     9901 tttgttaggt tgggtttgtc atgagtagct tggcaacttg ctaaaaaaat tgaaaaaaca
     9961 ataagtagtt tacgcatatt ctttgatatt tcttgttgtt atgagttatt gctaatatta
    10021 ccaaaaaata ataattttcg aagtgcgatt aaattaattc acaacttatt tagctttata
    10081 aaaaaaatat taatattttt aatatgccat ttggccgcct gcaaaaggct gtgataagat
    10141 acatttattt tgcaactata tcaggtaata tcatgaccag agcaacgtct tttctattga
    10201 cgctattatt ttttattact ttatttgtac agtctaccaa ttcaatggct gatgaagtgc
    10261 tcccctcctc tgatttgcag aagccttctc caacagtcac aacgaaacct tttacaactc
    10321 cagctgctcc attgcttaat gccaaagctt atattttaat tgatgtgaat agtggaaaag
    10381 taattgcaga aaagaatagt gaggaaaaat taccacctgc cagtttgacc aaaatgatga
    10441 cattgtatgt catttctaat gcattgaaga gtggacaaat tcatcttaat gatttggtaa
    10501 gagtgagccg tgatgcatgg cagacaggtg gctctcgtat gtttataaaa gagggtgatc
    10561 aggtctctgt tgaagatctt ttaaaaggca ttatagtaga ctctggtaat gacgcttgtg
    10621 tcgcaatggc agagcacctt ggtggttccg aaaatgcttt tacagacatt atgaatcagc
    10681 aagctcaaaa tctgggtatg aaaaatagtc attttacaga cagtactgga ttacctgatc
    10741 ctaatctcta tacaactgct aaagatttgg cgattttagg tcgagcctta atacttgatt
    10801 tccctgagta ttatgactgg tataaacaaa aatggtttac atataacggt atacgccaac
    10861 ctaataggaa ccgtttgttg tggcgagata gccaagttga cggtataaaa acaggccaca
    10921 ccaatgatgc tggatattgt ctagtatctt ccgcaaagcg taataacatg cgtttattag
    10981 ctgtggtttt aggggaaccc agtgattctg tgcgtgctga tgacagtgaa aaattgctaa
    11041 attacggatt tagatttttt gaaacacatc aattatataa atctgggcaa tcaattgccg
    11101 agttaccgct ttacaaagga acttctgata aagttgctgt gggtttaaca gaagatcaat
    11161 ttattacgat acctgccggt caatatgaaa ggttaaatat ttcaactaaa atttctaatg
    11221 atttacaagc tcctataaag aaaggcgata aagttggcga tattattgtg caatttaata
    11281 ataatatcgt ttcaagcaaa ccgctttatg ctttgcaaac agtggagtca ggtggattct
    11341 ataccagaac aaaagattcg ataaggttag catttaagcg atggttcggt tcctaggata
    11401 ttttcataat gaatatcgca tttgttaacg gcaagtattg ttgtcaatct gaagcaaaag
    11461 tttcaatatt tgatcgaggt tttctttttg gtgattcggt ttatgaagtg ctgcctgttt
    11521 accatgggca gccttacttt gtagaccaac atcttaaccg attattctca aatatgaaaa
    11581 aaattaagat gactatacca aattatgatt ggcatggttt aattcataga ctaatatcag
    11641 aaaataatgg aggtaattta caagtatata tccaagtcac acgaggaaat caaggggtgc
    11701 gcaagcatga tatccctact tccatcacac cttctgttat cgcattcacc atgcataatt
    11761 catttcccac cctcgaagat aaggaacagg gaatgtcagc aaaactggtt gaagactttc
    11821 ggtggatgag atgcgatata aaaactactt ctttaattgc caatatatta ctgaatgacg
    11881 aggctgtatc tgcaggattc catacttcaa ttcttgcccg gaatggtcta attacagagg
    11941 gaagtagtac caacgtattc attgtcgccc aggatggcgt tatcaagacc ccgcctatga
    12001 ataatttctg tttaccagga attacgcggc aagttgttat tgaaataatt aaaaaattag
    12061 atttaaagtt cagagaaata gaaattagta tttcagagct tttttctgct caggaagttt
    12121 gggtaacaag tacgacaaaa gaagtattcc ctattacaaa gattaatgat tctttggtta
    12181 atagcggaaa agttggcgaa tattggcgga taattaatga ttcttaccaa caactagtaa
    12241 actaaattat gacaaaaacg actttaatag aattcccttg ctattttcct ataaaaatta
    12301 tcggcattaa ctcagaatta ttgctcaatg aaatcaggca aattgttatt aatcacttcc
    12361 ctacttttaa aaatgagcat ttaactcata aaacgagtaa acaagacaag tatttggcta
    12421 ttactgtgac agtatatgca gaaaatcaaa tctcgctgga tggattatat caggatttaa
    12481 gccaacatcc ggctgtgaaa atggttttat aatgataatt cataatttag gcataaaaga
    12541 ttatactgaa atatgggagc agatgaaggc gttcactgca atccgtgaca gtaattcttg
    12601 tgatgagctt tggttactag agcattaccc tgtatatact caggggcagg cagggaaacc
    12661 agagcatgta cttaatccca actccataaa aattgtgcaa tctgatcgtg gtggacaagt
    12721 tacatatcat ggcccaggcc aactagtagc ctatgtttta atggatattc gtcgcaggaa
    12781 cctgggcata agaactttag tagcaaaact ggaagaaata ttaatttcgg ttttaaagca
    12841 ttataaaatt ccagcaaaca ttcgcagtgg ggcacctggg gtttacgtag gtgaaaaaaa
    12901 aattgcatcg ataggactga gggtgaaaaa tgggtgtacc tatcatggta tagcattaaa
    12961 tgtgaacatg gatttgagcc cattcttagg tattaaccca tgtggttttg ctaaaatgga
    13021 aatgacacag atgagtcatt tccatcctaa tattcagtta gaggaagtaa gccaacattt
    13081 tgtacagtat tttttgactc aatttaaata ataggaaatt atgaatttgt tagcagatta
    13141 tgtgcaacca ctgacttatt ggctacaagc aaacccacat tggtcactgt ttattacttt
    13201 cttaatcgct ttaagcgaat ctttagctgt cattgggagt attatacccg gctctgttac
    13261 tatgactgca atagggatat tagcaggttc tggaatcatg cgtattgatt tgacattact
    13321 tgccgctact ctgggggcta tttgtggaga tagccttagt tatttcttgg gatatttcta
    13381 cagtgatcgt ttaattgaga tttggccttt tagaaaatac ccgggcttat tgaagtatgg
    13441 taaagatttt tttgcaagcc atggcggtaa aagtgtatta attggtcgtt ttgttggccc
    13501 attgcgttct atcattccag ttattgctgg aataatgcat atgaaacaat ggagattcct
    13561 gatcgcaaat atagtttctg ctatcggatg gtctatttta tatgttatgc caggggtgtt
    13621 gattggtgca gcaggacatg agttatcagc agaaggagct acaaggtttt ttatcttgat
    13681 attggtcatt cttgctgtta tttggttgag tagcctcgtt ctcaaatggt tatttattaa
    13741 gttaaattca tttttgagaa ataacttgca tcgtttttgg ttagagatta agacaagccc
    13801 cggattatct aaagtatatt atgctatcac tcctgttcag gaggaaaatc attacaatac
    13861 ggttggttta ttactttttg ctctcgtgat tttttttctc tttttaattt tagttatttt
    13921 taccattgaa ggtcaactac tagactatat taatgtgccc gtgcattttt ttatgcaaag
    13981 ctttcacagt tccttgctgg aagcagtttt tattgtatgt gctcaattaa caagtaaatc
    14041 aactttagct ggattattta tcgcttcttg tatatggttt atttatcata aaaattaccg
    14101 agctacggtt tatttaagca gcataaccct attgagcggt gttattgctt tatttttaac
    14161 taagattatt gatagcccca gaccgcaagg actattgatt actttgcctg gttcttcttt
    14221 tccatctgtt agtgtttcca tcgcaacagc actgtatggt tttattttat tttacataaa
    14281 tagtaagtat tccttaataa ccagcagttt taaaacagtt attttaattg tgctcggatt
    14341 aagtgggttt gctaatatct atttaggtga tttttggttt tctgatattt tagctgctta
    14401 tctgttggga gctgaagtat gtttgattca ttgtttggta tatagaaggt ttagcacttc
    14461 tgctataaag aatgtacatc ctttaattat gctcatatca ctgcttatgg ttattgtatt
    14521 tgcaacagct gtttcaacag ttgtgaattt taaagaactg gttcatgacc atatgcccta
    14581 tcacaaggag tttactttaa cagaaaaaca atggtgggat cagcaaagac ctcttttacc
    14641 tttgtacagg ctaagcagaa ttggaaaacg aataagtctc ttaaacattc aatatgtcgg
    14701 tgatctggat gcattgcaaa ataagctaga acaatttggt tgggaaccac agagcgataa
    14761 aacttttttt accagattgc tgatgcgaat gaatagtgca ccatcaaacg ttaaactgcc
    14821 tttactcact caattatatg aaaataaaaa accaggcttg ataatgacgt atcaagataa
    14881 aaaatcaaag ttgatttttg atttgactat ttggcaatcg aactattatt tatctgagcc
    14941 taaaagaatg gtgtggatag gctccattca tcaaaatatt tttaatttaa agcaaaaaaa
    15001 atccaagcat aatttaccac cagtattaaa tccattgtct tatgtaatcc ctgctcttga
    15061 tgactttacc cttagaagga ttgcgctaac tgatgatttg gtaaagacat cagttttccc
    15121 tactgctccc tttattcttt tgataaaaac caaatagtag agcatagttg attacagtaa
    15181 gcatgttctg atttatttat attaggcctg gctagtaacc catgtaaatt actgatataa
    15241 ctatatatgt tcttataatt tatataataa gggatggaat caatgcatat cttggctaaa
    15301 gttattcact gtatctttcc attaattgcc ctaatacttt taattattgg aatcaaacga
    15361 aacgcgattt attatattat ttcttcgtta tgggtaagcc ttatttcatt agtcattcat
    15421 tatcagactt ccggtggaga gattttaggt acttatttta attatatcaa cgcaacaata
    15481 tataccatca atttgttcgt tttgtgttta tctttggttt atgttttatg gcatttaagt
    15541 aatgataatg tagcgtttaa aattgttagc agtttgttcc aatcatttat agtcattgga
    15601 gctttattgg ttgtaattaa cctttggatt aacgcttatt ttatcgaaaa caggttgaca
    15661 ggaactcctg tgatgcaggt agctctttta gagaaacctg aatattgtga ttatagatat
    15721 atattttata aggtggcact ggatggtacc gttcactatc tttgccctaa tcattacggt
    15781 ttaatcccat cgattggtcg attgtctata agtccagatt ttatagcatc gcagttatca
    15841 ataccaagca aaaagcaaat gctcttattg caacaaaaaa ggagttaaat tagcgatttt
    15901 ctatatttta acaaattgtt ctgatattta ttatttgata gtatgtgtgt aacttaccag
    15961 tatagtgcgt ctatgaaacg atttttttcg atattgttta ttctttactg ctgtaattta
    16021 tttgcaatgg cacctaagct taactgggat aaagcgattg acaaagcaat aaaacgttat
    16081 ggactaagag tagaaccaca attaatttct tatttttcta aagccggggt tagctaccct
    16141 cctaaagaaa ttgccttgct tgccttcaag tcagagcgta aagttgagtt atgggcaaaa
    16201 aatccaagcc aggaatggac gcatattcat gattacccat taactggttt tagcgggcgt
    16261 ttaggaccca aattacgtga gaatgataag cagatccccg aagggatcta taaattagta
    16321 aattttaatc cttttagcag catgcactta tccatgatga ttaactaccc aaatagtttc
    16381 gatcggcaaa aaggttatat ggacggtagg aaagagttag ggaataatat ttttattcat
    16441 ggaaaaaatt tatctgtcgg atgtttggct gtcggtgacc ttgccattga tcaactgttt
    16501 atcttggcaa gaagagtcgg tctcaaaaat attcaggtta ttattgcccc taatgactta
    16561 cgatatcaaa agccatcaac ctctacattt gcccaaccca gatggttgcc cgagctttat
    16621 aagcaaataa cagaatcact caaacccttt aaaaagacta aatttactgc ttaatataaa
    16681 gattggtctt tttattttat ttaagtaatt tgagtatata cttaaataaa a
//
LOCUS       DATNSN010000065        16715 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.065, whole genome shotgun sequence.
ACCESSION   DATNSN010000065 DATNSN010000000
VERSION     DATNSN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16715
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.065"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            80..1153
                     /locus_tag="VAH76_001680"
     CDS             80..1153
                     /locus_tag="VAH76_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HEO1386307.1"
                     /translation="MMSVDNIKNDNQVLAEEDFLEYALSHGFGDLHFKVDPQTGMKAM
                     IAIHSTKLGPALGGCRFIEYPNTAAALKDAMRLARGMSFKAASVNLPLGGGKSVIIKP
                     KGFFNRSEYMHRFGEFVNELNGRYITALDSGTELSDMDIIAEHTDYVASLSRYNGDPS
                     PSTAKGVLRGIQAAVAFKLGKDSLKGLHIAIQGLGHVGYALAKQLHELGATLTVADIS
                     PAAVNMAVQELGAKAVGTDIIHKIPCDVFAPCALGAIINDLTIPQLQTSIIAGAANNQ
                     LAHTYHGKILHEKGILFAVDYVINAGGLIFAASKYLQTPEDKVSEQIDSIYASLTEIF
                     TRSAKDNLPTSEIADTLAKEKLA"
     gene            1168..1824
                     /locus_tag="VAH76_001681"
     CDS             1168..1824
                     /locus_tag="VAH76_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386308.1"
                     /translation="MKHLSLTPELYEYLLDISLREHPALAALRKETSTMELANMQVAP
                     EQAQFMQMLIRLTRAKKVLELGTFTGYSALAMSLALPDDGQVITCDINEGWTKHAHPY
                     WREAKQEHKIKLRLGPALDTLHSLLNEGGEHQFDFIFIDADKTNYLNYYELALKLVTP
                     KGLIAIDNIFWDGKVIDPNDTSGQTREIKKLNQVIKNDSRVFVSLLAIADGMFLVQPI
                     "
     gene            1891..2937
                     /gene="hppD"
                     /locus_tag="VAH76_001682"
     CDS             1891..2937
                     /gene="hppD"
                     /locus_tag="VAH76_001682"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEO1386309.1"
                     /translation="MQNNNPCGLDGFAFLEFSGPDRNKLHQQFSEMGFQAVAHHKNQD
                     ITLFKQGEIQFIVNAASHCQAEAHASTHGPGACAMGFKVKDAKAAFQHAIAHGGIAFQ
                     DAPHANHGLPAIQAIGGSVIYFVDEEHQPFSHEWNITSSEPVVGNGLTAIDHLTHNVY
                     RGNMDKWASFYASIFNFQEIRFFNIKGKMTGLVSRALGSPCGKIKIPLNESKDDLSQI
                     EEFLHEYHGEGIQHIALNTNDIYKTVNGLRKQGVKFLDVPDTYYEMINDRLPWHKEPL
                     NQLHAEKILIDGEADPKDGLLLQIFTENIFGPVFFEIIQRKGNQGFGEGNFQALFEAI
                     ERDQVRRGTLKELT"
     gene            2993..3991
                     /locus_tag="VAH76_001683"
     CDS             2993..3991
                     /locus_tag="VAH76_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEO1386310.1"
                     /translation="MKLASLKSTRSRDGELCIVNRLLTAAIRVPHIASTLQYALDHWD
                     VVEPKLQLVYQQLNDNQIEDAFAFAPELCASPLPRAYQWADGSAYVNHVELVRKARGA
                     EMPADFWTNPLMYQGGSDAFLGPRDPILVSSEAYGIDFESEVAVITDDVPMGIDHTNA
                     AHHIKLLMLVNDVSLRNLIPEELAKGFGFFQSKPASSFSPVAITPDELGSYWDGQRVN
                     LPLISHFNGQLFGQPNAGIDMTFSFPELIQHAAKTRFLTAGTIIGSGTVSNLDRSKGS
                     SCIAEKRMLEMIEFGQAKTPFMRFGDRIRIEMLDGQGHSLFGSIDQIVTKYEKDSL"
     gene            3988..4626
                     /gene="maiA"
                     /locus_tag="VAH76_001684"
     CDS             3988..4626
                     /gene="maiA"
                     /locus_tag="VAH76_001684"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEO1386311.1"
                     /translation="MILYDYFRSTACYRVRITLNLKKIVYEKIEVHLVNNGGEQHSLQ
                     YHQINPQELVPSLDINGHILSQSMAIIDYLEEVYPEIPLLPKDPFMKANLKSLALIVA
                     CDMHPLNNLRVLNRLKEQFRASQEQVLEWYHHWLKTGFDAFEEKLGTMKRDKPVCLGS
                     EVSLADVCLIPQVYNAQRFHFDMASYPIINQINEYCLTLPAFHDAAPEAISS"
     gene            complement(4623..5183)
                     /locus_tag="VAH76_001685"
     CDS             complement(4623..5183)
                     /locus_tag="VAH76_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386312.1"
                     /translation="MLQRVLLVLLCFFSFLASASDTVLKLYRPFGDVFEQAPPVVKKI
                     MSGQCYEQSRIIIREDAWLCQAEGQLFDPCFVKAGSKKMEAVCPQSPWVGDSVQINVG
                     IPLNNEHHKTLDMSRAFPWAIELVNGEYCLAVNSNEQYDSMPVRYRCSNQNVLIGYLQ
                     RCKTAWSMLEKTSKGVITVELRRAWF"
     gene            5331..6734
                     /gene="asnS"
                     /locus_tag="VAH76_001686"
     CDS             5331..6734
                     /gene="asnS"
                     /locus_tag="VAH76_001686"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO1386313.1"
                     /translation="MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHD
                     GSCFSPIQIVATDQLSNYHKEVTKLTAGCSMVATGKLVASQGKGQFFEIQAESIEVVG
                     WVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
                     VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVE
                     AYCMAMSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCK
                     TVLEERADDMDFFNQFVAPGCIERMEHIADSEFEMMTYTDAIKALEASDQKFEFPVSW
                     GLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVS
                     NVRDVIPFPRTPGHADY"
     gene            complement(6731..7129)
                     /locus_tag="VAH76_001687"
     CDS             complement(6731..7129)
                     /locus_tag="VAH76_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386314.1"
                     /translation="MDEKKDKTIQINEITSTVEDEKKAQPLRKSERSLPVKSHRRSFS
                     IFYPYSAENSKISPSAQTSNKGFLPGPDFNREHYDTVDAGGPLAGNADDKILEKIFSP
                     RNRRRLLPESHQPQQQTVEVDDDSFRPLPI"
     gene            complement(7153..7836)
                     /gene="lolD"
                     /locus_tag="VAH76_001688"
     CDS             complement(7153..7836)
                     /gene="lolD"
                     /locus_tag="VAH76_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEO1386315.1"
                     /translation="MNDIILTSQKLYKSYHDGTSTVEVLKGVDLAITKGDRIAIIGPS
                     GSGKSTLLHLLGGLDKPTSGLITLGKVDWQKINEKQRCQLRNQQLGFVYQFHHLLPEF
                     TALENVMMPLLLAGMAVKDAEEKAINMLEQVGLKPRLAHKPAQLSGGERQRVAIARAL
                     VHQPHCVLADEPTGNLDEATASKVFDLMLELNKKMNTALVIVTHDQRIAERMDRVLVL
                     HEGSLYARE"
     gene            complement(7829..9076)
                     /locus_tag="VAH76_001689"
     CDS             complement(7829..9076)
                     /locus_tag="VAH76_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEO1386316.1"
                     /translation="MYKPLALFIGLRYTRSRKKNHFVSFISLSSMLGIGMGVMVLITV
                     LSVMNGFDQEIHRRFFGMAPEITITGPDERLSDWPEVVKKVETIPGIKAIAPYIGSQG
                     LLTHEGQVLPIVLTGILPEKEQSVTHLNKKLLAGNMDNLKHFGIILGKGLADSLGVMI
                     GDKVTIMIPQATVTPAGMIPRFKRFTVVGVFSAGTGFNFDTKLAFINIEDAQKLMQMD
                     KNDVSGIKMKINNVYKAPELSYELSDLLGEGYQVGNWTQQFGAFFEAVKMEKTMMFMI
                     LLLIIAVAAFNLVSSLVMVVNDKQAEIAILRTIGATPSTILWIFIVQGMMVGLVGTIL
                     GLLGGLVLANNATEIVNALQSFFQVKVLSSSIYFVDYLPSKIMFRDLWQVCAMALLMS
                     FAATIYPAWRASKTVIAEALHYE"
     gene            complement(9517..9603)
                     /locus_tag="VAH76_001690"
     tRNA            complement(9517..9603)
                     /locus_tag="VAH76_001690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9567..9569),aa:Leu,seq:taa)
     gene            complement(9620..9693)
                     /locus_tag="VAH76_001691"
     tRNA            complement(9620..9693)
                     /locus_tag="VAH76_001691"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9659..9661),aa:Cys,seq:gca)
     gene            complement(9754..9829)
                     /locus_tag="VAH76_001692"
     tRNA            complement(9754..9829)
                     /locus_tag="VAH76_001692"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9794..9796),aa:Gly,seq:gcc)
     gene            complement(9882..10445)
                     /gene="pgsA"
                     /locus_tag="VAH76_001693"
     CDS             complement(9882..10445)
                     /gene="pgsA"
                     /locus_tag="VAH76_001693"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO1386317.1"
                     /translation="MSTLTSLPNILTLMRISIIPVFIMVFYFPFKWAHPTAAVLFAIA
                     SITDWLDGYLARKLEMMSHFGAFLDPVADKLLVSTSLLLLVGAKDIDYITIPAIVIVG
                     REIVISALREWMAEVGSRASVAVGYIGKVKTSLQMIALVLLLAFNPLVSWWGSLGFVL
                     LYVSAILTIWSMVIYLAIAWPELMKKS"
     gene            complement(10466..11125)
                     /locus_tag="VAH76_001694"
     CDS             complement(10466..11125)
                     /locus_tag="VAH76_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO1386318.1"
                     /translation="MSSAYQLVIFDWEGTIADTLGVILHTVATEAKMLGFGDIDPYQA
                     RKYVDLGLVQAVKKTLPNLTAGQQEDLLQAVQSAMISRPTEVRLIPGVLEFIQQLHQA
                     KVDLAIATNKGHNSLIRALQATELDQLFKVTRSAGQVPAKPCPQMLEEIMEEFGRDPS
                     STLMIGDSTTDMEMAKRINVNAIGMDFYHQQEDALKAAGALAVFDDYKLLSAYLMLPE
                     A"
     gene            complement(11118..12065)
                     /locus_tag="VAH76_001695"
     CDS             complement(11118..12065)
                     /locus_tag="VAH76_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO1386319.1"
                     /translation="MSEVSYKEIGSEENGQRLDNYLIRILKGVPKSHIYRIIRGGEVR
                     INKKRAQANSRLQIGDSIRIPPIRMTDSKEIFVSDNLAKRLKDSIIFEDSSLLVINKP
                     AGIAVHGGSGLSLGVIEALRKTRHDLSYLELVHRLDRETSGCLLLAKKRSTLRAIQAL
                     LEAREVQKIYWALLTHRWDGKKKITITAPLEKNTLKSGERVVSVQKDGKASETDFKLL
                     ENYHQACWVEASPKTGRTHQIRVHSAYFGHVIVGDGKYGSKVGEVEGIDYQQNRLYLH
                     ARSIQFNLNGVMHVFQADVDDGFGNTLKQLRARSLLSYE"
     gene            12419..12616
                     /locus_tag="VAH76_001696"
     CDS             12419..12616
                     /locus_tag="VAH76_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386320.1"
                     /translation="MLGRKNKMRFHLSRRYKEATQYQSKIDNYFDRYLRMPDMLYTNY
                     ELLPGFCTPDERPFFSNPLNL"
     gene            12965..14956
                     /locus_tag="VAH76_001697"
     CDS             12965..14956
                     /locus_tag="VAH76_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEO1386321.1"
                     /translation="MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKA
                     IVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQEL
                     LIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
                     TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIR
                     SIRDNLRKSISEIIIDDQISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQI
                     ETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATALNTNLEAADEIARQ
                     LRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQR
                     LRLSLGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMAT
                     FIMNEKRNFIRDIEKRHGVSVVVIANPYMHPPQYTITRLKEDNVGKSKKPSYSLIQQP
                     EVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHTVTQEPG
                     KKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRK
                     KSNPNQQSHGGQQSNRSIPAKNEGNKKREAILAKEPEEK"
     gene            complement(15316..16593)
                     /gene="legC7"
                     /locus_tag="VAH76_001698"
     CDS             complement(15316..16593)
                     /gene="legC7"
                     /locus_tag="VAH76_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC7/YlfA"
                     /protein_id="HEO1386322.1"
                     /translation="MATNETELQVLIQHDSKSTITTSSLDSTDKDPKASTGTPEIESG
                     SLAQIVDTQKQLSQVKESLESIVDSIAENPSLITRAASAWGELPMWQKVTGGLVLTAP
                     TLAVGLFAHIGVLLVIGGVTGLTYTAGAIVLDDHHTCNVNIAKRLKEGLFGLADLLQI
                     TIEALDAIRKKFAEEIEKFKNENLRLTDNIDRLGNEVESLSAQVELYMEIEKMLRKDT
                     NEMEQTVKKLQESTTKQTDLLEKNQKELSKIRKEYEKSQLQLAEKIAELHEVRLSLGL
                     EVQKAKTVAKTLEGTVQTLTGTVIADEEQRVSFQKKLDGFLNDKQLSFDQVAERICKA
                     EEELKKVKEELRQSNDRYSELLKRQEQQVERLEKLGLHKLERIVDKENVKPSNDPVHS
                     GLLSHGIYSTPKGKVTQPKVEVVEDNKTIALVN"
BASE COUNT         5086 a         3514 c         3183 g         4932 t
ORIGIN      
        1 gtttgctaag aattacattg atttaattga cgaatagatc tataacttct cccaaaatta
       61 ttgcaagagg agcttaaaaa tgatgtctgt tgacaatatt aaaaatgata accaggtttt
      121 ggctgaagaa gatttcctcg aatacgcatt gtctcatgga tttggtgatt tacattttaa
      181 ggtggatccg caaacgggta tgaaggctat gattgcaatt catagcacca agttggggcc
      241 tgctttgggt ggttgtcgat ttattgagta ccctaatact gctgctgctt tgaaggatgc
      301 tatgcgcctt gccagaggca tgagctttaa ggctgcttca gtcaatttac ctttgggcgg
      361 tggaaaatcg gttattatta agcctaaggg tttctttaat cgatcggagt acatgcatcg
      421 gtttggtgaa ttcgttaatg aattgaatgg caggtacatc actgctttgg atagcggtac
      481 tgaattaagt gacatggata ttattgctga gcatactgac tatgttgcta gtttatccag
      541 atacaatgga gatccttcac cttctactgc taagggtgtg ttaagaggaa ttcaggctgc
      601 cgttgccttt aaattaggta aagattctct caaaggtttg catattgcta ttcaaggtct
      661 tggccacgtc ggttatgctc tggcaaaaca attacacgaa ttgggcgcga cattaactgt
      721 tgctgatatt tcacctgctg ctgtgaacat ggcggttcaa gagttgggag ccaaagcggt
      781 cggcactgac attatccata aaataccgtg tgatgtattt gctccttgtg ctttaggtgc
      841 gattatcaat gatcttacta ttccccagtt acaaaccagt attatcgctg gcgctgccaa
      901 taatcagctg gcacacactt accatggcaa gattttacac gaaaaaggca ttttgtttgc
      961 tgtggattat gtgattaatg caggtggttt gatattcgca gcctctaaat acttacaaac
     1021 gcctgaagac aaggtaagtg agcaaattga tagtatctat gcctctctga ctgaaatttt
     1081 tactcgttct gctaaggata atttacctac tagcgagatt gctgatactt tggctaagga
     1141 aaaattggct taatttggat accgtttatg aaacatttat cccttactcc tgagttgtac
     1201 gaatacctgc ttgatatttc cttgcgtgaa catcctgctc ttgcggcatt acgcaaagaa
     1261 acatccacta tggaactcgc caatatgcag gtagctcctg agcaagccca atttatgcag
     1321 atgctgatac gtttgactcg tgcaaagaag gtattggaac ttgggacatt cacaggttat
     1381 agtgccttgg ccatgtccct tgctttgcct gatgatgggc aagttatcac ctgtgatatt
     1441 aatgagggat ggacaaagca tgcccatcct tattggcgtg aagccaaaca ggaacataaa
     1501 attaaattac gccttggacc tgctcttgac actttacact cattacttaa tgaaggtgga
     1561 gagcatcaat ttgatttcat ttttattgat gctgacaaaa ccaattatct gaattactat
     1621 gaactggctt taaaattggt tacacctaaa ggattaattg ccatagataa tattttttgg
     1681 gatggaaaag taattgatcc gaatgatacg agtggacaaa ccagagaaat taaaaaatta
     1741 aatcaagtga ttaaaaacga ttcaagggtc tttgtcagtt tgttagctat agctgatgga
     1801 atgtttctgg tgcaacccat ttaatcttta aaatccccgg caagcaatac actgcattgc
     1861 tatgtaaata agtacaagga gaatctgata atgcaaaata acaacccctg cggattagat
     1921 ggctttgcct ttttagagtt ttcaggccct gataggaata aattacatca acaattttct
     1981 gagatggggt ttcaggccgt tgcccaccat aaaaatcaag acattactct tttcaaacaa
     2041 ggggaaatac aatttatagt gaatgcggct tcccattgtc aggcagaagc gcatgcttca
     2101 actcatggtc caggcgcttg tgcaatgggc tttaaagtaa aagatgccaa agccgcattt
     2161 caacacgcta tcgcgcatgg cggtatagca tttcaggatg cacctcatgc caatcacggc
     2221 ttgccagcca tccaggctat tggtggtagt gttatttatt ttgtcgatga agaacaccaa
     2281 cccttctctc atgaatggaa tattacctca tcagaacccg tagttggaaa tggtctgacc
     2341 gcaatcgacc atctcaccca taacgtttat cgcggtaata tggataaatg ggctagtttt
     2401 tatgcttcca tttttaactt ccaggaaatt cgttttttca atatcaaagg aaaaatgact
     2461 ggtttggtca gtcgagcatt aggtagccct tgtggcaaaa tcaaaattcc tttaaacgaa
     2521 tccaaagatg atttatcaca aattgaagag tttcttcatg aatatcatgg cgagggcatt
     2581 caacacatcg ctctcaatac caatgatatt tataagacag tcaacggctt aagaaaacaa
     2641 ggggtcaaat tcctggatgt gccagatact tactatgaga tgattaatga ccgcctccca
     2701 tggcacaagg agccactgaa tcaactccat gctgagaaaa ttttaattga tggagaagca
     2761 gatcccaaag acggcttgtt actgcaaata tttactgaaa acatatttgg accagtcttt
     2821 tttgaaatta ttcaacgcaa aggcaatcag gggtttggtg aagggaattt ccaggctcta
     2881 ttcgaagcca ttgaaagaga tcaagttcga cgtggtactt taaaagaatt aacctagaca
     2941 aacagttgaa atgtaatgat gctgtttttc aggcataagc caggagatct atatgaaact
     3001 cgccagtttg aaatcaactc gttctcggga tggagagctt tgcattgtca acagattgct
     3061 gaccgctgca atacgcgttc ctcatatcgc atcaacactt caatatgccc tggatcattg
     3121 ggatgttgtt gaacccaaat tacaactggt ttatcaacag ttaaatgata atcaaatcga
     3181 agacgccttt gcgtttgctc ccgagctatg tgcctcgcca ttaccccgtg cttatcaatg
     3241 ggccgatggc agtgcctatg taaaccatgt cgaattggtt cgtaaagcca gaggagcaga
     3301 aatgcctgct gatttttgga ccaatccact catgtatcaa ggcggatctg atgcttttct
     3361 ggggcctcgt gatcctattt tggtaagctc agaagcctat ggaattgact ttgaatccga
     3421 ggtggctgtt atcactgatg atgttcccat gggaattgat catacaaatg ctgcccatca
     3481 tatcaaactg ttgatgttgg ttaatgatgt ctctctacgc aatcttatac ccgaggaact
     3541 ggcaaaaggg tttgggttct tccaatcaaa acctgccagc agcttctccc cggtagcgat
     3601 aaccccggat gagctaggtt cttactggga tggacaacgt gtgaatttgc cattaattag
     3661 tcattttaat gggcaattat ttggccaacc caatgctgga attgatatga ccttttcttt
     3721 tccagaactg atacagcatg cggcaaaaac aaggttttta actgcgggta ctattatagg
     3781 ttctggaaca gtatccaacc ttgaccgcag caaaggttct tcttgtattg ctgaaaagcg
     3841 aatgctggaa atgatagaat tcggacaggc aaaaacaccg tttatgcgat ttggggaccg
     3901 aattcgcata gaaatgctcg atggtcaagg gcattcccta tttggctcca ttgaccaaat
     3961 agtgacgaaa tacgagaagg attccttatg atattgtatg actatttccg ctctaccgca
     4021 tgctatcgag taagaatcac tcttaattta aaaaaaattg tttacgaaaa aattgaagtg
     4081 catctggtta ataatggagg cgaacagcat agccttcagt atcatcaaat taaccctcaa
     4141 gaacttgttc ccagcctcga catcaatgga catatactga gccaatcaat ggcaatcatt
     4201 gattatctgg aagaagtcta cccggaaata ccattgcttc ctaaagatcc ctttatgaaa
     4261 gccaatctaa aatctctggc tttaattgtt gcttgtgata tgcaccccct caataattta
     4321 agagtgttga atcgcttaaa ggagcaattt agagccagtc aagaacaggt actggaatgg
     4381 tatcatcatt ggctaaaaac aggatttgat gcgtttgagg aaaaacttgg gactatgaaa
     4441 cgtgacaagc cagtctgcct tggaagtgag gttagtctgg cagacgtttg tttgatcccc
     4501 caggtttaca atgcccaaag atttcatttt gatatggcga gttacccaat aatcaatcaa
     4561 atcaatgagt attgtctaac cctgccggca ttccatgatg cggcaccaga agccatttca
     4621 tcttaaaacc atgctcttct caactccacc gtgatcacgc ctttagaggt tttctccaac
     4681 atactccagg ctgttttaca tcgttgcagg taaccaatta aaacattttg attgctgcaa
     4741 cggtacctta caggcattga gtcatattgc tcgttagaat ttacagccaa acagtactca
     4801 ccattaacca attcaatagc ccagggaaaa gcgcgagaca tatctaaggt cttatgatgt
     4861 tcattattca aaggtatccc tacgttaatt tgtacgctat caccaaccca tggagattgg
     4921 gggcatacgg cctccatttt tttgctacca gctttcacaa aacaaggatc aaataattga
     4981 ccttcagcct gacaaagcca cgcatcttcg cgtataatga tgcgggattg ttcataacat
     5041 tggccagaca ttattttttt aacgacaggt ggtgcttgtt caaaaacatc accaaaaggt
     5101 ctgtataatt ttagaacagt atcgctggcg ctggctaaaa aagaaaaaaa acatagcaat
     5161 acgagtaaaa ctctttgcag catggtgttc ccccacggat tttatttagt gtatcatcac
     5221 attttaattc agggtttagg aaatactaca aacgggagaa aatcaatccc tcgaaggatt
     5281 ggaatatttc ctaagccctg aatcaatcgt tttcgatttt ggagtatata atgacagaag
     5341 tctttactat aaaacaatgt ctcgacggcg aaatcagtat tgatgaaacc gttacggtaa
     5401 gaggatgggt gaaaactcga agagattcaa aagctggctt gtctttcatc agccttcatg
     5461 atggctcttg cttttctccc atacaaatcg tcgctactga tcagttatcc aactaccaca
     5521 aagaagtaac aaaattaact gctggttgct caatggtagc gactggaaaa ttggtggcat
     5581 cgcaaggaaa agggcaattt tttgaaatcc aggccgaatc cattgaagtg gttggatggg
     5641 ttgaaaaccc agatacatat cctattcaag ccaaacgcca tactcttgaa tttttaagag
     5701 aagtagctca tttgcgcccc agaaccaata ccataagcgc tgtcaccaga gtgcgtcatt
     5761 ctttggcgca agccattcac cgtttttatc atgaacaagg ttttttttgg gtgcatacac
     5821 ctatcattac tgccagtgac tgcgaggggg caggagaaat gttcagagta tccactctgg
     5881 atttattaaa tatccctaaa aatgataaag gacaaatcga tttcagtaag gatttttttg
     5941 gtagagaaac gttcttaact gtttctggcc aactaaatgt cgaagcttat tgtatggcca
     6001 tgtccaaggt ctacactttt ggaccaacat ttcgggctga aaattcgaat acaagccgcc
     6061 atttggctga attctggatg atcgaacctg aaatcgcttt tgcaaatctg gaagacattt
     6121 gtaaactcag ccaaagtatg ttgcgttatc tctgcaaaac agttcttgaa gagcgtgccg
     6181 atgatatgga ctttttcaat caatttgtcg cgccaggatg tatagaacgt atggaacata
     6241 ttgcggattc tgaatttgaa atgatgacct ataccgatgc gataaaagca cttgaggcga
     6301 gtgaccaaaa atttgaattt ccagttagtt ggggattaga tctccaatca gagcatgaac
     6361 gttacctggc tgaagtactt tgcaaaaagc ctgtcattgt gacaaattac ccgcaagaaa
     6421 ttaaggggtt ttacatgcgt cttaacgacg atgggaaaac ggtagccgcc atggacgttt
     6481 tagctccagg catcggagag attatcgggg gcagccaacg cgaagagcga ttggaaatac
     6541 tggatagacg catggacgaa tgcaacctga ataaagaaca ttaccaatgg tatagagatc
     6601 tgcgccgtta tggcactgtt cctcatgccg ggtttggctt gggctttgaa cgtctgatta
     6661 gctatgttac aggagtatcc aatgtgcgag atgtgatacc tttccctcgc acaccaggtc
     6721 acgcggatta ttaaatcggt aaaggtctaa aagagtcatc atcaacttca actgtttgtt
     6781 gttggggttg gtgactctct ggcagcaatc gccgtctatt tcttggcgaa aatatttttt
     6841 ctaatatctt atcatctgca ttacccgcaa gcggtcctcc tgcatcaact gtatcataat
     6901 gttccctatt gaaatcaggt ccaggtagaa atcctttgtt acttgtctga gctgaaggag
     6961 atatttttga attttcggct gagtaaggat aaaaaataga aaaacttctt ctatgtgact
     7021 ttacaggtag actacgctcg gattttctca atggttgggc ttttttttcg tcctcaacag
     7081 tgcttgtaat ttcattgatc tgaattgttt tatctttttt ttcatccatt gttaacactc
     7141 cttatcttat ttttattctc tcgcatacaa actgccttca tgcaaaacaa gtactctatc
     7201 catacgttct gcaatccgtt gatcatgagt tactataacc agggctgtat tcattttttt
     7261 attaagttcc aacatcaaat caaaaacttt actggctgtt gcctcatcca aatttccagt
     7321 cggctcatca gccagaacac aatgtggctg atgcaccaaa gctctcgcta tggcaacccg
     7381 ttgcctttct cctccagaca actgagcagg cttatgcgct aatcgtggtt taagccctac
     7441 ctgttccagc atattaatgg ctttttcttc cgcatctttc acagccatac ccgccagtaa
     7501 taacggcatc atcacattct caagtgccgt aaactcgggt aataaatggt ggaactgata
     7561 aacaaatcct aattgttgat tacgcaattg acaacgttgc ttttcattta ttttttgcca
     7621 atcgactttc ccaagagtaa tcaggccgct ggttggctta tccagcccac ccaacaaatg
     7681 caaaagggtg ctctttccag aacctgatgg cccgataatt gcgatcctgt caccttttgt
     7741 tatggccaag tcaactcctt ttaacacctc gactgtagat gtgccatcat ggtaggactt
     7801 atacagtttt tgacttgtta aaatgatgtc attcataatg caaagcctcc gcgatgactg
     7861 tctttgatgc tcgccaggca ggataaatgg ttgcagcaaa actcattaat aacgccatgg
     7921 cacaaacttg ccacagatca cgaaacataa ttttagaggg caaataatca acaaaataga
     7981 tgcttgaaga caataccttc acctggaaaa aggactgcaa cgcattaact atctccgttg
     8041 cattattggc caacactaaa cctcctaaca acccaagaat tgtaccaacc aggccaacca
     8101 tcattccctg tacaataaaa atccataaaa tggtagaagg agtagcgcca atagttctta
     8161 aaatagcgat ttctgcttgt ttgtcattga ctaccattac cagagaggac accaaattaa
     8221 aagctgccac agcaataata agcaataaaa tcataaacat catcgttttt tccattttaa
     8281 ctgcttcaaa aaaagctcca aattgctggg tccaattgcc tacttgatat ccttctccca
     8341 aaagatcaga taattcataa gacaattcag gtgctttgta tacattatta attttcattt
     8401 ttatacctga aacgtcattc ttatccattt gcattaattt ttgagcatct tcgatattaa
     8461 taaatgccaa tttagtgtcg aaattaaatc ctgtaccagc agaaaaaaca cctacaacgg
     8521 taaagcgctt gaatcgaggg atcataccgg caggagttac tgttgcttgc gggatcatta
     8581 tcgtaacctt atccccaatc atcaccccaa ggctgtccgc aagacctttt ccaagaataa
     8641 tcccaaaatg tttcagatta tccatattac ctgccagtag tttcttattg agatgagtta
     8701 ctgattgttc tttttctggc agaataccgg tcaggacgat aggcaaaacc tggccttcat
     8761 gagttaataa tccttgactt ccaatataag gtgcaattgc ctttattcca ggaattgttt
     8821 caaccttttt aaccacttca ggccaatcac ttaatcgctc atcaggacct gttatggtga
     8881 tttcaggcgc catcccaaaa aaacgacgat gaatttcctg atcaaaacca ttcatcactg
     8941 ataaaacagt gattaaaacc atcactccca tgcctatacc caacatggaa cttagggaaa
     9001 taaaggaaac aaagtgattt ttcttacgtg aacgcgtata acgtaatccg ataaaaagag
     9061 ccaatggttt atacataatc gtattcagct ggattaaagt tagtattgta cagcacttat
     9121 agaaaaggcc aagattgaag cctaaagatt ttaaagaact tattaataat tcatcatcag
     9181 acaacccaca tagttatcca cagaatctgt ggatatctaa taagtcccat tcatcgatta
     9241 gagcatcaat taagcaaatg ccaacttaca taaaatgaac atccgttacg atatagcaga
     9301 tgctacacat aaccagaaaa ttattaaatg gcactggtat ttgtcagtga aattttaaaa
     9361 ttaatcagga aatgcattat ttaaatggag aaaagaagtt attaggatgc ttcgttttac
     9421 gaagaataat caaataacta aagggtttca ttggaagatg aaattgattc aaggtagggt
     9481 tatcattgcc attttaaaat ggggattagt caaatttggt gcccagggcc ggaatcgaac
     9541 cggcatggtg ttaccaccga gggattttaa gtcccttgcg tctacctgtt tcgccacctg
     9601 ggcattcact gtcttttaat ggaggctgag gccggaatcg aaccggcgta cacggctttg
     9661 caggccgctg catgaccact ctgccacaca gcctcaaatc tatatacttc gctattgctc
     9721 taaaaaaaaa gcgacttaga tgtcgcttaa atttggagcg ggaaacgaga ctcgaactcg
     9781 cgaccctaac cttggcaagg ttatgctcta ccaactgagc tattcccgcg tctctacggg
     9841 atgagattct accgaaaata aataccctgt caacagcaaa attaactttt tttcattaac
     9901 tctggccagg caatagccaa ataaatcacc atagaccaaa tagtcaaaat agcagacaca
     9961 taaagaagaa caaaccctaa gctcccccac caggaaacaa ggggattaaa tgccagcaat
    10021 aataccaaag cgatcatttg caaagaagtt ttaactttcc ctatatatcc aacggcaaca
    10081 ctagcacgac ttccaacttc agccatccac tcgcgcaagg ctgaaatcac aatttctcgt
    10141 ccaacgatta cgatagcagg tatagtgata taatcaatat ccttcgctcc aactagcaat
    10201 aacaagcttg tcgataccaa taatttgtcg gcgacgggat caagaaaagc tccaaaatga
    10261 gacatcattt caagtttacg cgccaaataa ccgtctaacc aatcagtaat gcttgcaata
    10321 gcaaaaagaa cagcagcggt aggatgagcc catttgaaag gaaaataaaa taccataata
    10381 aatactggaa tgatcgaaat ccgcattaag gttaatatat taggcaaact ggttaacgta
    10441 ctcactgctg aacatccccc cttttttatg cttccggcaa catcagataa gctgacaata
    10501 gcttataatc atcaaataca gccaaagcac ctgctgcttt aagcgcatcc tcttgctgat
    10561 ggtaaaaatc catgcctata gcattaacat tgattctttt tgccatttcc atatccgttg
    10621 tagaatcacc tatcatcaaa gttgatgaag gatctctccc gaattcctcc atgatttcct
    10681 caagcatttg cggacaaggt tttgctggta cttgtccagc agagcgtgtc actttaaaca
    10741 attgatcaag ctcagttgcc tgaagagcac ggattagaga attgtggcct ttatttgtag
    10801 cgatggccaa atcgaccttt gcttgatgaa gttgttggat aaattctaat actccaggta
    10861 ttaaacgcac ttctgttggc cgtgaaatca ttgcggattg cactgcctgt aacagatctt
    10921 cctgctgccc ggcagttaga ttgggcaatg tcttttttac tgcttgaacc aatcctaagt
    10981 ctacatattt tcttgcttga tatgggtcaa tatcaccaaa gcccaacatt ttggcttcag
    11041 tagcaacagt atgtaaaata actcccaatg tatcagcaat cgtgccttcc caatcaaaaa
    11101 ttactaattg atatgcacta ctcatagctc aataggctcc ttgcacgcag ttgctttaaa
    11161 gtatttccaa atccatcgtc tacatcagct tgaaatacat gcattacccc attcagattg
    11221 aattgaatag accttgcatg cagataaaga cgattctgtt gataatctat accttccacc
    11281 tcacccacct tagagccgta tttcccatca ccaacaatga catgaccaaa gtaagcgcta
    11341 tgaacgcgaa tttgatgtgt ccgtcctgtt ttaggagagg cttctaccca acaggcctga
    11401 tgataatttt ccagcaattt aaaatctgtt tctgatgcct taccatcttt ctgaacggaa
    11461 acaacccttt ctccggattt caatgtgttc ttctcgagag gagctgtaat tgtgattttc
    11521 tttttaccgt cccaacgatg cgttaataaa gcccaataaa ttttctgcac ttcgcgggct
    11581 tccaatagcg cttgaatggc tcgtaacgtg cttctttttt tggctaataa aagacaaccg
    11641 gaagtctctc tatccaatcg atgtaccaat tccaaatagg ataaatcgtg ccttgtcttt
    11701 cttaatgctt caataacccc caggcttaaa ccactaccac catgtactgc aatacctgct
    11761 ggtttattaa ttaccaacaa ggaagaatct tcaaaaatga tactatcctt caagcgtttt
    11821 gccaaattat cacttacaaa aatttcttta gaatcagtca ttcgaattgg cggtattctt
    11881 atactgtccc caatttgtaa ccgactgtta gcttgtgctc tttttttatt aatccgaacc
    11941 tcgccgccac gaataatcct gtagatatgg cttttaggaa ctccttttaa aatgcgaatc
    12001 agataattat ccaaacgctg gccattctct tcggagccaa tttctttata acttacttca
    12061 ctcattgtca ataaacctgt tttctgagcg tgattttttt gatatgatta gatatcgtac
    12121 gggaaaatcc cttacgaccg aattataacg tataaataga aaaatagttt aataatcgat
    12181 tcataatcga taaaagattt gtaattaatt agttaaaata cttgcgttca tcttaatttt
    12241 tcatcattaa tgaagaatga aaaattaaca gaatagcata aatttagtta atttgatagc
    12301 aaatcgaact aaagaagcag ctgctcaagc gaatataaaa caggttgaat tcaataagaa
    12361 cttgaaccac aagttaaaga atcacctctg tttttttcat cgtaatacgc ctgagcagat
    12421 gcttggaaga aaaaacaaaa tgcgcttcca tttaagtcga agatataagg aagcgaccca
    12481 ataccagagt aaaattgata attatttcga cagataccta cgcatgccag acatgcttta
    12541 taccaattac gaattattgc cgggcttttg cacgcctgat gaaaggcctt ttttttccaa
    12601 cccacttaat ctgtaaacgg tacatcatac agtgtgcacc tttaactact gaggtaacat
    12661 gcgtacggtc tgtccacaag gtcatgggtc agcttaaagt tcctcaccct gccagcttaa
    12721 taacattgtt tttcaaaaac aaaattgagc aattagggaa taggctgtta tcatttcacc
    12781 agaattatag agaccaggat attaattttc tgactccctc tctctgcaga ttcaagacga
    12841 atgcaagata tgggacccgg aaaattaact tcatggcaag aaaactgtag aaatgatcat
    12901 acggctatac tgagccttac actatctcga tgtaaatgca tgcgccctaa atatggggtt
    12961 atacatggaa aaaatgctaa tcaatgcaat gcaaacagaa gaaattcgtg ttgcactaat
    13021 taaaaacaat caattatttg atctggacat tgaatgcccg ggagaaataa agaaaaaagg
    13081 taatatttat aaagccattg taacaagaag agaacccagc cttgatgctg ttttcgttga
    13141 atatggttcc aaacgccaag gtttcttacc cctaaaagaa atagcgcctg aatatttaag
    13201 caagaatccg gaagagtttg gagatgagaa acctccaatt accagcctca tccgtgaagg
    13261 tcaagaatta cttatccaag tggataaaga agaaagaggg aataaaggag cagcactgac
    13321 aacttttatt accctggcag gttgctacct ggttttaatg cccaataacc ctcattcggg
    13381 cggaatatca agacgtattg aaggtgatga gcgtgacgaa ttacgcgaaa cattaaatgc
    13441 tttaaccctc cctgaagaca tgggccttat tattcgcact gcaggtgttg gaaaaagtct
    13501 ggaagaactg caagatgact taaacatgct ttgcaatcag tggcaatcca tcaaggaagc
    13561 ttataattca gaactggcac cttgcctgat tcatcaagag ggcgatgtca ttattcgttc
    13621 cattcgtgac aatttgcgaa aatccatcag tgaaattatt attgatgatc aaatatccta
    13681 tatcaaagca aaacaataca ttgaacgcgt aaaaccagaa tttttaccta atctaaaact
    13741 ctataacagc agcatccctc tttttaattt ctatcaaatt gaaagccaaa ttgagacagc
    13801 ttatcagcgc caggtcatgc ttccttctgg aggtgccttg gtcatagata gaactgaagc
    13861 tctggtatca attgatatta actcagccaa ggctacaggc ggtgccgata ttgaagcgac
    13921 tgcattaaat accaaccttg aagcagctga tgaaattgct cgccaacttc gcctgcgtga
    13981 tttaggtggt ttggtagtta ttgattttat tgacatgagt tctagtaaaa atcaaagaga
    14041 ggtagaaaat cgactcaaag aagcactgca ggctgatagg gccagaattc aagtagggcg
    14101 aatttcacgc tttggccttc ttgaaatgtc aaggcaacgc ctccgattat cattaggaga
    14161 aactgcccag gaaatttgtc cacgttgtga aggtagagga actgtacgca gtatacagtc
    14221 tcatggctta tcaattaccc gccttattga agaagaggcg ctcaaggaaa aaacagctga
    14281 gattcaagtg caattgccac cagaaatggc tacattcatt atgaatgaaa aacgtaattt
    14341 tatcagggac attgaaaaaa ggcatggtgt cagtgttgtt gttattgcga atccttatat
    14401 gcaccctcca caatatacga ttactcgcct gaaagaagac aacgtaggca aatctaaaaa
    14461 accaagttac tctttgattc agcaacctga agtacatgtt cccagcagtg ataaagaacc
    14521 cgtgtcacat gatgaacctg ctgtgaaatc atttgccgaa cacagggtca tcaaacaccc
    14581 tcaaaccagt tttattaaac gattatggac cagtttattt ggtggtcatt cagttgccca
    14641 caccgtaact caagaacccg gtaaaaaaca tcatcgtgct caaaatcaag gtaaacacgc
    14701 atcgtcaaaa agctcaggag gagacaaacg tcaacaaggc agcaacgcaa atcgtagacg
    14761 tcgttcagga ggaggccagc aacgttcttc atcaggaaat acaaatgctg cagccagcac
    14821 acaccagcaa cgaaaaaaat ccaacccgaa tcagcaaagt catggtggcc aacaaagcaa
    14881 tcgttcaatc cctgcaaaaa atgaaggcaa caaaaaacgg gaagccatac tggctaagga
    14941 gccagaggag aaatagggca tattaattat ttgcctattc accttttcaa taagcgttta
    15001 tccgcacagt ccagttacct ctggattgtg tggataaaac acagcactct ttcttgtcag
    15061 gataccgatt ttaaaattgt aactattcac aggatatgca aatttttctg tttataagcc
    15121 tgtttatttg agtcgtcttt cccgcaaagt cgggaatcta tccataaagc tagtatatag
    15181 gttaattttt agatggattc ccgccttcgc gggaaagaca gaggacttaa tattctttga
    15241 ttgcatatcc cttaaccggt tacttcaaat tataaccttt aaaacagcct gattcttctt
    15301 ccttaaatcc ttctcttaat tgactaaagc aatagtctta ttatcctcta ctacctctac
    15361 tttaggctgg gtgactttac ccttgggcgt actatagatt ccatgggaaa gcaaacctga
    15421 gtggacaggg tcattactgg gtttcacatt ctctttgtct actattcttt ccaatttatg
    15481 caagcccaat ttttccaaac gttcaacctg ttgttcctga cgctttaata attcactata
    15541 acgatcattg ctttgtctta attcttcttt gactttttta agttcttctt ctgccttgca
    15601 aattcgctca gctacttgat caaagctgag ctgtttgtca ttcaggaatc catccagttt
    15661 cttttgaaaa gaaacacgtt gctcttcatc agcaatgacc gttccagtta aagtctggac
    15721 tgtaccttcc aacgttttag caactgtttt agctttttga acttcgagac ccaacgacag
    15781 tctgacttca tgtaattcag ctattttttc ggctaactgc aattggcttt tttcgtattc
    15841 ctttctaatt tttgacaatt ccttttgatt tttttccaat aaatcagttt gttttgttgt
    15901 agactcttga agttttttga cagtttgctc catttcattt gtatctttac ggagcatttt
    15961 ctcaatttcc atatataact caacctgagc actcagtgat tccacttcat tgcccagcct
    16021 atcaatatta tcagttaatc tgaggttttc attcttaaat ttctcaattt cttcagcaaa
    16081 ttttttgcga attgcatcca atgcctcaat cgtaatttgt aacagatctg ccaaaccaaa
    16141 aagtccttct tttaatcgtt tggcaatatt cacattgcat gtatggtggt cgtccaaaac
    16201 aattgcacct gcggtatagg ttagtcctgt aactccccct attactaata agacaccgat
    16261 gtgcgcaaat aatcctactg ccaaagtagg cgctgtcagg acaaggccac ccgtgacttt
    16321 ctgccacata ggcagttcac cccaggctga cgcagcgcga gtaatcaggc tgggattttc
    16381 cgctatactg tctactattg attccaggct ttctttaact tggctcagtt gtttttgggt
    16441 atcgacgatt tgggcaagac taccgctttc aatttcagga gtgccagtac ttgccttagg
    16501 atccttatca gtactgtcca agctagaagt agtgattgtt gatttgctat catgttggat
    16561 caatacttga agctctgttt cattagtagc catatttact ccttacgatt aaaaatcttc
    16621 cttagatgtc catttttaat cataaattat caataagaca agtaattctt gcattttttg
    16681 caattaaatt ttattttaat aaacctacta caaaa
//
LOCUS       DATNSN010000066        16605 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.066, whole genome shotgun sequence.
ACCESSION   DATNSN010000066 DATNSN010000000
VERSION     DATNSN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.066"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            43..1797
                     /locus_tag="VAH76_001699"
     CDS             43..1797
                     /locus_tag="VAH76_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1386323.1"
                     /translation="MSFSKVNKILESNSILKEEIDKPEVLGLAQSSGGEEPPMIPDEM
                     LFALQDRVGVNRVQQACIDGNLHVLERMNCDEFDYVDSYGTTPLMLAIEHDHKAIAYY
                     LLRSRMINLNLHKKDNAGRSALFMAKEKGWDDIVDLIRNTMENPVYNNMELYYATING
                     YLDSVTELLPKIDEPGFATKETWINTYPLFGNIFHAACCGGSLEIVKLLMEHGAEINS
                     RSYVRERHNFYYATTPILVACRYGHPHLVEYLLTQDGVQYDIIELFHIAVEYGHYAVM
                     DLLLRKIRAENIALDLNRGLEFHASWYGTPLDKACRSGQVDIVDRLIAEGIDMKEEDN
                     ISAITTAIWNADNPEYPAIDILDRLLAECDRQGISIPLTAPEIAIIACNRNRADILDR
                     LIIRGFDFNLGNRHGEYPLITAAIKGHEAIVDRLLSLSVDVNQRDSEGRTALFQAACH
                     NNLEIVKLLVDKGADINIPDNKGVTPLMRVAETCFTEVVKYLLNSGADTSLKDKGGRS
                     ALDYASSSSLYFMQENLALIRDADIQKNLYKSSSSSSQSVNNPYMLWSSNRANLPEEE
                     SVKSEIISEEKAFTFQSK"
     gene            complement(2012..2917)
                     /locus_tag="VAH76_001700"
     CDS             complement(2012..2917)
                     /locus_tag="VAH76_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1386324.1"
                     /translation="MHMSDIKSFLAVVEFHSISLASKRMHISQPTMTRRIQKIEQELG
                     TPLFFSSGIRTELTAKGKELLPYFRQMLDIYQQMYINVGNKTKNPLKLINVGTSVYVA
                     NSIIQDFMHSAEISSSNYFFNIKLLAEPHIIDSLNSGEYDLIIAPPVYDCSSSVVSIP
                     LWKEKILAVVGASHPLTRNKKAISITELMSHDVALMEKGFTLRKKLDSLSEELHTPLK
                     AIFESNSIYNNIALIEKGVGWCLIHERLLNPNLIPVELSDCNMEIEFHCSFLKRREDE
                     RLIWQFLEQIRKWILADYLKQYACP"
     gene            complement(3216..4295)
                     /locus_tag="VAH76_001701"
     CDS             complement(3216..4295)
                     /locus_tag="VAH76_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386325.1"
                     /translation="MNALSLIRQMAEKPELNEFIQGIKEGNLTAEHVKLFCQATAQVG
                     MEATRGLWDTNVEQNDFSNDKNREAIYSASLLHGAHNIGHVNNLLHNKNIGISDRSKE
                     AIIGQMEDHSPIGSRRDLAWEKEIATGFLHDAINKFQEGRAKNAAKKGSGDKDFHDGV
                     VDMVGFVMHSLFGVGKAMGRGFLDSQERFSNSAQSNEPTINEHGQLINERLASTPQEP
                     YVQNIGSSKSSIGVSPQLNSTTQNSTIPNEIRKFVTGGLQFKNDDQQVVDNTIEDLKT
                     EVISAFELHNMVQDLPTSKSVQGIEAFKVEQEPSRSNDVTHYFKSILQQLKTTDPQPH
                     KGEIAAHNPGEDNESDISKSIRFRS"
     gene            complement(4793..6535)
                     /locus_tag="VAH76_001702"
     CDS             complement(4793..6535)
                     /locus_tag="VAH76_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1386326.1"
                     /translation="MSFSKISKTLENSATLIDEKAEQDASDLSHTLGSDSQLLISEES
                     SDVLLENTGENLVQQACIDGNLEKLKELLRGDMWGMDEVSLNQVNNRGETLLMLAIKY
                     GHKQMALFLARSLFHLHKIDNSGKSALSLAHEKGWEDIVQLILESINNREARIRELHY
                     AAIHGYVESVSQLLGIIDKYGFKPHGFILVGDETPRFENIFHAACCGGSLEIVKLLLE
                     HGADINSKSQFSENNYSHYPATPILVACRYGHANIVEYLLAQDGIEYDINELLHTAVG
                     FGHFAVLDLLLRHSKENRTRNLNMVGEYPLTHFRAPLLTRACYSGQVDIVDRLLAEGI
                     DMNNAENRQAFLTAIFCAKTQGSPAIDIIDRLLAENDRQNIDIPLDGFKDLVLWAYRQ
                     PFVGILDRLIIRGVDFHRVRDPGDYPLIEAVKNGYKDIVHRLIDLSVDINLRDRKGRT
                     ALFCAVMQGEKEIVELLINNGADPNIQDNEGVTPLMKVVPTDNSEMVELLLNAGADTS
                     IKDKRNRLAIDLAKHFPYHMKLLKDATEIQNANKSSSSSNQSANALYTLWYSNRGDLR
                     EEAQFKEKNTPHPF"
     gene            7074..7823
                     /locus_tag="VAH76_001703"
                     /pseudo
     CDS             7074..7823
                     /locus_tag="VAH76_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611762.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            7874..8164
                     /locus_tag="VAH76_001704"
     CDS             7874..8164
                     /locus_tag="VAH76_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO1386327.1"
                     /translation="MHPSYVYILASQRNGTLYIGSTSDIINRVWEHKNKVIPGFTAKY
                     NVHMLVYYEMYDLYIDAARREKRFKNWPRQWKINLIEKFNPEWRDLYEEICQ"
     gene            complement(8408..10228)
                     /locus_tag="VAH76_001705"
     CDS             complement(8408..10228)
                     /locus_tag="VAH76_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEO1386328.1"
                     /translation="MIVIPHEVFVSKPNTALISMRYIVTTGNDYTLESEIMNVKPNIQ
                     TTLQNGKPLPYQKIGNQKVASFKDPNKPPYGPFANTTNAGEFPLKQTVLIHGIEYSFT
                     WPSSEHAFHAQKLIHLMNKLGINDPNQIHILNTLRKIEKTQNQAGKQFDPRENWDPIA
                     RELATNMGITGTDQDKKDKIDELCDSNYYSKALVKKGIVPGTKEPYTRRFMATVLRLK
                     CDQNPELKELAMRCASEGILPIEASTKDVNWASGIDGSGRNMLGIEILKLGNQYLMEA
                     GRGNEIKIADPDKAYEDLQRSHQKDLGHDAIIGKPWSNPLNNSLNIWYDSTRKQTVVE
                     SHRGSNLQLIYKDGEPPKAIWRKDENSPWQDGDFKRPAVQSLLQAYQDAGSPVPTVAK
                     QQPHPAQLNDRWAKVHKQHHDNPVINLPLFKQELREGVKLELNKIEAELKKGINPLFS
                     RNESHLERIVREYVNPAVLEASKILAAYSVKNAMHDLNTSVTVSMVDNNRAHDDKHPL
                     HAMKIQFKDNAEAKQFAEKLLNEYGIHSHTFGKGIMKTPQNGAIYLTPKDLQMLSQLS
                     GLAKDPLAGLQAFANMAKNYDKAKEVSIKEDLESHAGLSP"
     gene            10667..11419
                     /locus_tag="VAH76_001706"
     CDS             10667..11419
                     /locus_tag="VAH76_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386329.1"
                     /translation="MFSKILKSRPNFFKAISEICNPGIGDKILKLQKPSLLIRIDGRS
                     PDSLHIQDGFQPRVSGERLKSVTASDVEDYQKFNVNPFGWGACQSLKEIMTFMQKNKS
                     QTDKGWLYKFFGKATPLLILKQEVGADSHGHDDEKEVIITEHIPLKNFIASIAPEDRE
                     MFMDGREIPNAMYSYNSSLPKSLSKQDLLTEQDILAWLLRHDAEEPFTDLCQTVFTDM
                     PQDILEYLFQENPGIVSAITSAIEKNMVLNLL"
     gene            complement(11877..11981)
                     /locus_tag="VAH76_001707"
                     /pseudo
     CDS             complement(11877..11981)
                     /locus_tag="VAH76_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
     gene            12173..13207
                     /locus_tag="VAH76_001708"
     CDS             12173..13207
                     /locus_tag="VAH76_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEO1386330.1"
                     /translation="MIDNTVKIIKHKVGLLNLAEELGNVSKACKVMGLSRDTFYRYKS
                     AVESGGIDALFDQNRRKPNIKNRVDESVELAVKEYAIAFPAHGQQRTSNELRKQGIFV
                     SPSGVRSIWLRYELANFKDRLKALEAKVTSEGIILTEAQVSALEKKKFDDEACGEIET
                     AHPGYLGSQDTFYVGTLKGVGRIYQQTFVDTYSKVAFAKLYTTKIPITSADILNDKVL
                     PFFEQYNLPILRILTDRGTEYCGKVEHHDYQLYLAINNIDHTKTKANSPQTNGICERF
                     HKTILQEFYQVTFRKKIYESIDELQKDLDEWMDYYNNQRTHQGKMCCGRTPMQTLIED
                     KQIWMEKFIN"
     gene            complement(13273..14484)
                     /locus_tag="VAH76_001709"
     CDS             complement(13273..14484)
                     /locus_tag="VAH76_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386331.1"
                     /translation="MAKFDVSQSQLWTILNAQQNKKQHTNSPSKEVKKFDMSEAQLWA
                     ILNAQQNKKQQISSPSDEKGDSYIKFFNKHSREHLKKEYDPNGNANQPSKIIKDAQSS
                     IGALSRIFDEYDCPTPNYEEINTIYQKPNGLREHIQENLKGIIPVLLNEDRTALHPDV
                     IEAIGPENYKAILKNAQGDERAIAQQFVQAVILGYGQRIYQNTDDQNLKDNLYNTVLP
                     ALGKLAEKVTLQGLPEQSRELVPADILDTLIMSRGLLKLLNEANTAGVEIPNCRSMKD
                     KLGTVFDVMDPKNEDYNMLPEATKICKARENYDEAINSFITGVRDILKKDEIPKPKEV
                     SWFKKFLRFITSNEKLLQNADEKRYDQQFKVLSKIEDLNKKLNSLSQEKNISSEHEHT
                     HEHGNGNRMTF"
     gene            14768..14941
                     /locus_tag="VAH76_001710"
     CDS             14768..14941
                     /locus_tag="VAH76_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386332.1"
                     /translation="MLPEYLLCLPKVLGYGLNIALRNTSAPTIQLRKGIIMLRYQCYA
                     WGIGDINTYKMFN"
     gene            14990..>16605
                     /locus_tag="VAH76_001711"
     CDS             14990..>16605
                     /locus_tag="VAH76_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386333.1"
                     /translation="MPHSTNQSNVNINQSNVIENINQYLKWHNLPVRFGTGGVCNGLS
                     TVHAQYVLQGREREFFKLLRYVAGDKGILDADDSVKEKVNDFVWKVVASHMTSGHDKE
                     LNQLNSFKTLSVNNKPLKSVFDLPLVTSDENWGTILKDLKLKEDEVMLVRSINHTISI
                     TRKGNQYHVYDPNYEKEAEPFSSEQEVIKELHERVFHYNKGNMDNKGNMGLILSIITP
                     EDKEPRQDLPKPVDIYGKYLDKENVDSSATITVEHPLGKWDLTTKTIDLACECGDENL
                     IKKLIELHQFDVKNRPINQAYKAVQYNNVRTAELLVNDMIRILPKQDQVEQDELSDIP
                     TEELPEEFLKQFEEIPDAPEGYEIKEAEVQNPKVVMSPSEKKIKDLLYPLILKAASYG
                     RKEVFDTLWDIRNGYDLHLMSELPQNTEQYKNSYLFIKKGDTRELYYIKPDGKDEKVE
                     INDFNLFEEKINAIKNENEIKLRVGEKQIQEIITSCGGSALLNKNADDFDKEDLKDFI
                     LSAAKGGNSVLLQELIDKYYESNKTLYDKD"
BASE COUNT         5131 a         2962 c         3183 g         5329 t
ORIGIN      
        1 aattgtaaac taaagtccat taattttgat ggagaaaata ggatgtcatt tagcaaagtc
       61 aacaaaatac tagaaagcaa ttcaattctt aaagaagaaa tagataaacc ggaagtcctt
      121 ggtttagccc aaagttcagg tggagaagaa ccaccaatga taccagatga aatgctattt
      181 gctttgcagg atagagtagg tgttaaccgt gtgcagcaag catgtataga tggaaactta
      241 cacgtattgg agaggatgaa ctgtgatgag tttgattatg ttgacagtta tggcaccaca
      301 cctctgatgc tagccattga acatgatcat aaagcaatcg catattattt gttacgaagt
      361 agaatgatta atcttaattt acacaagaaa gacaatgcag gtaggtctgc cctttttatg
      421 gcaaaagaaa aaggttggga tgatatagtt gaccttattc gtaacactat ggaaaatcct
      481 gtgtataata atatggaact ctactatgct accataaatg gatatttgga cagtgtgact
      541 gagttattgc ctaaaatcga tgagcctgga tttgccacaa aagaaacttg gataaatacg
      601 tatcctcttt tcggaaatat tttccacgcg gcttgttgcg gtggtagttt agagattgtc
      661 aaattattga tggagcacgg cgctgaaatt aactccagaa gttatgtccg cgagcgacat
      721 aacttctatt atgctacaac accaatatta gtggcatgcc gctatggtca tccccatctt
      781 gttgagtatt tattaacgca ggatggagtc caatacgata tcatcgagtt atttcatatt
      841 gcagttgagt atggtcatta tgcggtaatg gatttattgc ttcgtaaaat tagagcagaa
      901 aatatagcac tggatttaaa ccggggtttg gaatttcatg catcctggta tgggactcct
      961 ttggataaag cttgtagatc gggacaagta gacatagtgg atcgcctcat tgctgaaggt
     1021 atcgatatga aggaagaaga taatatatca gcaattacaa ctgctatttg gaatgcagat
     1081 aatcctgaat atccggcaat agatattctg gatcgcttgc ttgccgaatg cgatcgtcag
     1141 ggaatcagca taccccttac agcccccgag attgctatta tcgcttgcaa taggaatcgt
     1201 gcagatatac ttgatcgctt gattattcgt ggctttgatt ttaatctggg caatagacat
     1261 ggagagtatc ctttaattac agcagctata aagggacatg aggctattgt tgaccgcttg
     1321 cttagtttgt ctgttgatgt taatcaaagg gatagtgagg gtaggacagc tctattccag
     1381 gcggcttgtc acaataatct cgaaattgtt aaacttttag tggataaggg cgcggacatc
     1441 aatatacccg ataataaagg tgtgactcct ttgatgcgtg tcgctgagac ttgtttcaca
     1501 gaggtggtta aatatttatt aaactctggt gcggatacaa gcttaaaaga taaggggggg
     1561 cgctcagcac tggattacgc tagttcttcc tctttgtatt ttatgcaaga gaacctggct
     1621 ttgattaggg atgctgatat tcagaaaaat ctctataagt catcaagctc ttcatcccag
     1681 tcagtcaata atccatacat gctgtggtcg tcaaatcgag ctaatttgcc agaggaagaa
     1741 agtgttaaat ctgagataat ctcagaagag aaagcattca cttttcaaag taaataactt
     1801 tatcttgaga ttctatccca tgaccatata aaaatcggtc atgggaagct gtctcaaaga
     1861 aattttaacg gttacaggat ttacgaaaaa cctttaggtc aaggcaaaaa atttttgatg
     1921 gggattttag ataaactaaa tttgacctga cagacccttc ttgaaaaacc ggtaactctc
     1981 agatccagtg tgaaactact acacataatg tttatgggca tgcatattgt tttaaataat
     2041 ctgccaaaat ccattttcga atctgctcta gaaattgcca aatcaaacgt tcatcctctc
     2101 tcctttttaa aaacgagcaa tgaaattcaa tttccatgtt gcaatctgat aactccacag
     2161 gaattaaatt tggattgagc aagcgttcat gaattagaca ccacccaacc cctttttcaa
     2221 tcaatgcaat gttattataa atgctgtttg actcgaaaat agcttttaga ggagtatgaa
     2281 gctcctccga caaagaatct aattttttac gcagggtaaa tcctttttcc atcaacgcaa
     2341 catcatgaga cattagctcg gtaattgaaa tggctttttt atttctcgtc aaaggatggc
     2401 ttgctccaac cacagctaaa attttttctt tccataaggg gatagataca acagatgaag
     2461 aacaatcata tactggtggg gctataatta agtcatattc tccactgttt aagctatcta
     2521 ttatatgagg ttctgctagt aatttaatat taaaaaaata gttggaggaa gatatttctg
     2581 cgctgtgcat gaaatcttga ataatggagt tagcaacata gacagaagtt cctacattaa
     2641 taagctttaa tgggtttttc gttttattgc cgacattaat atacatttgt tgataaatat
     2701 caagcatctg acgaaagtaa ggtaaaagct cctttccttt tgctgtaagt tcagtcctta
     2761 tcccggaaga aaaaaacaag ggcgttccca attcctgttc aattttttga atccttcttg
     2821 tcatcgtagg ttgggatatg tgcattctct tagacgctaa tgatatggaa tggaattcaa
     2881 caaccgcaag gaaagacttg atatctgaca tatgcatatt tatgtttacc tcataccatt
     2941 gagagtgtgg tcgcattaat tgtttaacat aagtattgtg ctataccata aaaatatcaa
     3001 taaaccatgt tcgattttga tatgaccacc taaaattagt aaattttatt tcgagtttca
     3061 ggatgattat tttgctcttt tcccgaagag catctgagaa gctgtaatac attataactg
     3121 ttgatgtaag cgagtggttt agctgaaaaa gtcgaagggg ttaagctgat ctgtaagtta
     3181 cttcttgaaa aagtaactta cagattgagt ttgggctaac ttcgaaatct aatagattta
     3241 gatatgtcac tctcattgtc ctcaccagga ttatgggcag caatttcacc cttgtgtggt
     3301 tgtggatctg ttgtcttaag ttgttgtaat attgacttaa aataatgggt aacatcattg
     3361 cttcttgagg gttcttgttc aactttaaaa gcttctattc cttgcacaga tttagaagta
     3421 ggtagatctt gcaccatatt atgtaattca aatgcgctaa ttacttcggt tttcaaatct
     3481 tcaattgtat tgtctacaac ttgctgatca tcatttttaa actgaagccc tcccgtaaca
     3541 aattttctta tctcatttgg aatagtagaa ttttgagtcg ttgaatttaa ctgaggacta
     3601 accccaatag agcttttact ggaaccgatg ttttgaacat aaggttcttg aggtgtgcta
     3661 gctaatcttt cgttaattaa ttgtccatgt tcatttatag taggttcatt actctgggct
     3721 gagttggaaa agcgctcctg actatctaaa aaacctctcc ccatagcctt tccaacacca
     3781 aaaagtgaat gcatgacaaa ccctaccata tctacaacac catcatgaaa gtctttatct
     3841 ccagatcctt ttttagcagc atttttagct cttccctcct gaaatttgtt tatggcatca
     3901 tgtaaaaaac ccgtagcaat ttccttttcc catgcgagat ctctcctgct tccaattggt
     3961 gagtgatctt ccatctgtcc aattatggct tcttttgatc tatcactaat tccgatgttt
     4021 ttattgtgga gaaggttatt aacatggccg atattatgcg caccgtgtaa tagactcgct
     4081 gaataaattg cctctcgatt tttatcatta ctaaaatcat tttgctctac gtttgtgtcc
     4141 caaagtcctc ttgtagcctc cattcctact tgagctgtgg cttgacaaaa taattttaca
     4201 tgctcggctg ttaaattgcc ctcttttata ccttgtataa attcatttaa ctctggtttt
     4261 tcagccattt gcctaataag tgataatgcg ttcattttgt atcctccact gtatgttctt
     4321 ttattttaca aaatgagcat tattgtatca ttatgagcgc attcgtattt ttaatggctt
     4381 atacgcaatt tggtaatcat gatagcaaag tgttgaagga aaattattac agtgtaattt
     4441 tttatcttgt actaggcata agacgttaag tattctcgtg cttgggtaaa gagttaactt
     4501 agttcttata ttttacgctc tatccaaaag gaagataatc gattgatgat aactgtcagg
     4561 tttccatgcg ctgaatttat cctttagtag atatggtatt gttatctgtg gcgctaagat
     4621 gctaatttgc aaagactttt gtcttccttg acttgatttc aaaatgattt aaatgattga
     4681 ttatccatgc tggtgctatt ggttgaaatc tacaacgggc actcttgcga tatgatgagc
     4741 ccttatctga tattttggtc agataaaggg cagcaggggg caactgtttc ctttaaaagg
     4801 gatgtggggt atttttttcc ttgaattgtg cttcttctct caaatccccc cgatttgaat
     4861 accatagcgt gtacaacgca ttggcggatt ggtttgacga acttgacgat ttgttcgcgt
     4921 tttgtatttc tgtggcatcc ttcaataact tcatgtgata tgggaaatgc ttggcaagat
     4981 caatagctaa gcgattcctc ttgtctttaa tgcttgtgtc tgcaccggca tttaataaaa
     5041 gttcaaccat ctcgctgtta tcagttggaa caactttcat taaaggagtc acgccttcat
     5101 tatcttgtat attgggatcc gcaccattat tgattaatag ctcaacaatt tctttttctc
     5161 cctgcatcac tgcgcaaaaa agtgctgttc tacccttcct atctctcaga tttatatcca
     5221 cagacaaatc gataagtcgg tgaactatgt ccttgtatcc atttttgaca gcctcaatca
     5281 atggataatc tccggggtcg cgtactcgat gaaaatcaac accacggata atcaaacggt
     5341 caagaattcc tacaaacggt tgtctgtacg cccaaagtac aagatctttg aacccatcaa
     5401 gaggtatgtc tatgttctga cgatcatttt cggcaagcaa tcggtcaatt atatctattg
     5461 caggtgagcc ttgagttttt gcacaaaaaa ttgctgtcaa aaatgcttgt ctgttttcag
     5521 cattattcat atcaatacct tctgcaagca ggcgatcgac tatatctacc tgtcctgaat
     5581 aacaagccct tgttaaaagg ggcgcacgaa aatgggtcaa aggatattcc ccaaccatat
     5641 tcagattccg ggtcctattt tctttactat gacgaagcaa taaatctaat accgcaaagt
     5701 gaccaaaccc aacagccgta tgcaataatt cgttaatgtc atactcaatt ccatcttgag
     5761 ccaatagata ctcgacaata ttagcatggc cgtaccggca agctacaagt ataggtgtgg
     5821 caggataatg gctataatta ttttctgaaa attggctttt cgaattgata tcagcgccgt
     5881 gctctagcag taatttgact atctctaaac taccaccgca acaagcagca tgaaagatat
     5941 tttcaaatct gggggtttca tcgccaacta atataaaccc gtgtggttta aatccatact
     6001 tatcaatgat gcctaacaat tgagagacac tttctacgta cccatgtatt gcagcatagt
     6061 gcaactccct gattctagct tcacgattat ttatactttc caaaataagt tgaactatat
     6121 cttcccagcc tttttcatgt gctagagaca gcgctgattt acctgaatta tctattttat
     6181 gcaaatgaaa gagagaacgt gccagaaata gagccatttg cttatgccca tatttaatgg
     6241 ctagcattaa gagcgtttct cctctgttat tcacttgatt gagtgaaact tcatccatgc
     6301 cccacatatc acctctaaga agttctttta atttttctaa atttccatct atacaagcct
     6361 gttgaaccaa gttttctcct gtattttcca acagaacatc tgagctttcc tctgaaatta
     6421 ataattggct gtctgatccc aaagtatgcg ataaatcaga tgcatcctgt tcagcttttt
     6481 catctatgag tgtcgcacta ttttcaagag tcttactgat tttgctaaat gacattcatt
     6541 ttctccaaca aggtcaatca gccttaagtt acagtctgta tattaataaa acattaacca
     6601 gacatttaaa ttttttatgc cttatgcaat atttgttgat tacaatagga caattcaatg
     6661 ctggtgttca gaaaccaagt tagacaaaca gaataattga tttatatagg caaagtccct
     6721 gaagtttttt agacagtatt gaatcttgaa ttgagcacgg attattcaat ttcactccga
     6781 tctgctcctt ttcctgcgcc aggagtttta atagcatgat gatgccttgc gcgggagagg
     6841 gttggggaga ggggaatggg aaaatctttg caaagacaac gagctcgcga tttacggaaa
     6901 aacagtacca atacctaggg tctgttggca tttcacctgc cgcctacgtg ccgcggcttg
     6961 tccgcggcat ccagtagatg ttcaaatcaa aaaccgcact acctaattca ttgaataata
     7021 agagcaaact cataatataa cggttctgac cccaagaata aaacgagttt gcaatgctaa
     7081 gactaatgct caatgatgag ttttggtcga agctaaagga gagcatgcta cagcatggga
     7141 tttatgacaa gcctaatctt cgtatggtag tggaggcaat gttatatcgt atacgggttg
     7201 gctgcccttg gcgcgacctg ccatcataat ttggatgttg gaactccaat ctatcaaaaa
     7261 tttaatcgat ggtcatcaaa aggtaaattg atgtgcctat ttaaagaact tgttcaaaaa
     7321 ccagatcttg aatgggagtt tattgacggc agcattgtaa aagcgcacca acatagtgcc
     7381 ggcggagctt ctgaagaaaa tcaagctata ggaaaatcta gagggggcaa tacaacaaaa
     7441 attcatatgg ctgttgatgc tcatgggtta cccatcgact ttgaaataac tggaggtgaa
     7501 gtgcacgatt gtaaagtggc tccagaattt attgaaaaac tggctgcagc aggtcacacc
     7561 attgctgata aaggctatga cagtgaagaa gtgagagaca gtattcgtaa aaaatcatcc
     7621 actccagtta ttccaagaaa gagtaattct aaagttgcaa tatagatatg gactggtgtc
     7681 tttataaaca taggcattta gttgaaaatt tattcgctag aataaagcat ttccgtgcaa
     7741 ttgctacaag atttgataaa ttaaagcaaa attatgcttc tgttgttgct atggcgtatt
     7801 tatttttatg gctaactatg tagcatttct tgcagtatta tctggatgtt tgtccaacat
     7861 ccaaggaaat atgatgcatc catcttatgt ttatattctc gcaagtcagc gtaatggtac
     7921 tctttacata ggttctacgt cagacatcat taatcgagtc tgggagcaca aaaataaagt
     7981 cattcctggc tttactgcaa agtataacgt acatatgctt gtctattatg aaatgtatga
     8041 cttatatatt gacgcagctc gtcgtgaaaa acgtttcaaa aattggccaa gacagtggaa
     8101 aataaattta atcgagaaat ttaatccaga atggcgtgat ctgtatgaag agatttgcca
     8161 atgatttaga tctaatggat gccgcggaca agccgcggca cgtaggcggc aggtgaaatg
     8221 tcaacagacc ctagtgacca cgtcatcctg ttgagatgtt attatgctcc gaaaactcta
     8281 aaagaaaaat cctcacttga tattgctcag tgaggattga ttcttattag ttgaactgca
     8341 ttgcataatg acaaaattga ggtgattagt tctatagggt tcacactaat cacccttttg
     8401 cagagaacta tggactcagg ccagcatggc tttctaaatc ttcttttatc gaaacctctt
     8461 ttgccttgtc ataatttttt gccatattag caaatgcctg aagaccagcc aatggatctt
     8521 ttgccagtcc ggataattgc gacagcattt gtaaatcctt aggtgtcagg tatattgctc
     8581 cattttgagg tgttttcatt atgccctttc caaaagtatg actatgaata ccatactcgt
     8641 ttaaaagttt ttcagcaaat tgtttggctt ctgcattgtc tttaaattga attttcatcg
     8701 cgtgcaaagg atgtttatca tcatgagccc gattattgtc aaccatgctg acagtaacag
     8761 aggtattaag atcatgcata gcatttttta cactgtaagc agctaatatt tttgatgctt
     8821 ccaaaactgc tgggtttacg tattccctga cgatacgttc gagatgactc tcgttacggg
     8881 aaaataaagg attgattccc tttttcagtt cagcttcaat tttatttaac tccaatttta
     8941 ctccttcacg taactcctgt ttgaataagg gtagattaat gacggggttg tcatgatgtt
     9001 gtttatgaac tttagcccat ctatcattca gttgagcagg atgaggttgt tgctttgcta
     9061 cagttggtac aggcgagcca gcatcctggt atgcttgtaa taagctttgt acagcaggac
     9121 gtttaaaatc cccatcttgc cacggtgagt tttcatcttt tctccatatt gcttttggcg
     9181 gttctccatc tttatatatc aattgcagat tagaacctcg atgggattcc actacggttt
     9241 gctttctcgt ggagtcgtac cagatattta aagagttatt taaaggattc gaccatggct
     9301 taccgattat tgcatcatgc cccaaatctt tctgatgaga acgttggaga tcttcataag
     9361 ctttatctgg atcagctatc ttaatctcat ttccacgccc tgcttccatt aaatactgat
     9421 tgcctaattt taatatttca attcccaaca tatttctgcc agaaccatcg attccagatg
     9481 cccagttaac atctttagta cttgcttcta taggaagaat tccttcgctg gcgcaacgca
     9541 tagctaattc tttcaactca ggattttgat cacatttgag acgtaataca gtcgccataa
     9601 atcgacgtgt gtaaggttct ttggtacctg gaacaatccc tttctttaca agagctttag
     9661 agtaatagtt tgaatcacat aactcatcaa tcttatcttt cttatcttgg tctgtacccg
     9721 taatacccat attagtcgct agttctcttg caattggatc ccaattttct cttggatcaa
     9781 actgctttcc cgcttgattt tgtgttttct caattttcct taaagtgtta agaatatgaa
     9841 tctgatttgg gtcgtttata ccaagcttat tcattaagtg gattagtttt tgcgcatgaa
     9901 atgcgtgttc ggaggagggc caagtgaatg aatattctat gccatggatc aacaccgttt
     9961 gctttaatgg gaattcacca gcattagttg tattagcaaa aggaccgtaa ggtggtttat
    10021 ttggatcttt gaatgaggcg actttttgat tcccaatttt ctgatagggt agtggtttcc
    10081 cattctgtaa ggttgtttgt atgtttggtt ttacgttcat gatttcgctc tccaaagtat
    10141 aatcattgcc agtagttaca atgtaacgca tcgagattaa tgcagtatta ggtttcgata
    10201 cgaatacttc atggggtatg acaatcatgg atagctatgc atattatcgt cctcaaaact
    10261 ttcatggatc cggacaggta atgcaatagc ttatgtcatt aatcgatatc aggcagctat
    10321 agcgtttgaa gggttacact ttaagctctg atcatgcggc cttggcaggt gatgtaataa
    10381 ttcctggcca atcatgagct aatttcgtaa cgcattgcga acttaccgaa ttaatgaaaa
    10441 ggatttaggc atctgagctc attttttgag acactcgcat ttgatctttt tcactctctt
    10501 ctctggaaca ggattgttca caattcagaa atttagggtt cttataagtt tttgtgattt
    10561 gcgtataacc ccctccagaa atgtcataac caaataataa ttaattaatc aacatgaatt
    10621 aaaatcaacc aaaaatgcat tgattgttct ttattatgag gttattatgt tttccaaaat
    10681 tctaaaatcc agaccaaatt tctttaaagc catatccgaa atctgcaacc cagggattgg
    10741 tgataaaata cttaaattac aaaagccatc tttattgata cgcatagatg ggcgctcccc
    10801 tgattctcta catatacaag atggttttca gccgcgtgta tcaggagaaa ggctgaaatc
    10861 tgttactgcc tctgatgttg aagattatca aaaatttaat gtaaatcctt ttggatgggg
    10921 tgcatgtcaa tctttaaaag aaataatgac attcatgcag aaaaataaat cacaaacaga
    10981 caaaggctgg ttgtataagt tttttggtaa agctacgcca cttctcattt tgaaacaaga
    11041 ggtgggagct gacagtcatg gacatgatga tgaaaaggaa gtcattatca cagagcatat
    11101 tccactaaaa aattttattg cctcaattgc tcctgaagac agggagatgt tcatggatgg
    11161 aagagaaata cctaatgcca tgtatagtta taacagcagt ttgccaaaat cattgagtaa
    11221 acaagattta ttaacagagc aagatattct tgcctggtta ctaagacatg acgcagagga
    11281 accattcacg gatctttgtc agacagtatt taccgatatg cctcaagata ttttggagta
    11341 tctgtttcaa gagaaccctg gaattgtgag tgcaataacc agtgccatag agaaaaatat
    11401 ggttctcaat ttgctgtaaa aattatttta agctaggcaa cgcccctgaa gtatttttag
    11461 acaaaattta atcttgaaat gagcacatat ttttcaattc cacttcgacc tgccccctct
    11521 cccgcgatag gaactttggt atgatgatgt ctttcgctgg agagggttgg ggagagagaa
    11581 tgtcacggat ggtcattgat gacgaaatgt ggtctcgttt agaagaaact cccaaagcca
    11641 aaaggacgac acgataaaga tgaacggtta tctatggagg caatatgtaa gatgtttcgt
    11701 gctggtgcga catggcgtga cgtgacttgc caccgtacca tggcaactgg aaaagtgttt
    11761 ataatcgtta taataactgg tcgaaaaaag gttatatcac cacttagaag gattgctaca
    11821 aggtatgata aaacagccag aatgtacttc actggcttta tgtttttgag tatattttag
    11881 cataaaagta atgcagacat gataacttcg agggcagaag acgtttgctg aaaaacgtca
    11941 tgattccaaa agcgcaagac cttaaaccca tgagtcgtaa gtccagatct caataacctt
    12001 tcagagcaag gttattgagg tgggacgaaa ttacttccct agtaatcaaa gtggtttctc
    12061 taattttaga tgaattagtt tcgacttaat ctgacacaac tacttgaaaa agtgagagtt
    12121 tccggttttg atagaagtgc gaactttaaa caaaaccaag aggaaaactc tcatgataga
    12181 taatacagtt aaaatcatta aacataaagt tggcttatta aaccttgctg aagaattagg
    12241 aaatgtttct aaagcatgca aagtaatggg tttatccaga gatacttttt atcgctataa
    12301 gtcggcggtt gaatcgggag gcattgatgc tttattcgat caaaaccgaa gaaaacctaa
    12361 tatcaaaaat agagtagatg aatcagtcga gctagcagtc aaggaatacg cgatagcatt
    12421 tccagcacat ggtcagcagc gcacgagcaa cgagcttcgt aaacaaggta tttttgtttc
    12481 tcctagtggt gttcgtagta tttggttgcg gtatgaactt gccaatttta aagaccgttt
    12541 aaaagcatta gaggccaaag taacctctga agggattata ttaacagagg cgcaagttag
    12601 cgctttggag aaaaagaaat ttgatgatga agcatgtggt gaaattgaaa cagcccaccc
    12661 tggttacctc ggctctcaag atacttttta tgtaggtacg cttaaaggag tcggccgtat
    12721 ttatcagcaa acctttgttg atacttacag caaagtagct tttgccaagc tttatacgac
    12781 aaaaataccg attacctcag cagatatact caatgacaaa gtcttaccat tctttgagca
    12841 atacaacttg cctatactgc gaattttaac tgacagaggc acggaatatt gtggaaaggt
    12901 agagcaccat gattatcagc tttatttggc gattaataat atcgaccata cgaagacaaa
    12961 agcaaactca cctcaaacaa atggaatttg tgagcgtttc cataaaacaa ttttacaaga
    13021 gttctatcaa gtaacatttc gaaagaaaat ttacgaatca atcgatgaat tacaaaaaga
    13081 tctggacgaa tggatggatt actataataa tcaacgaact catcaaggta aaatgtgttg
    13141 tggtcgtact ccaatgcaaa ctttgataga ggataaacaa atctggatgg aaaaatttat
    13201 aaactaaatt tgacctgaca gacacttctt gaaaaaccgc taactgtcag atcaagtttg
    13261 aactactaca ttttagaatg tcatgcgatt tccatttcca tgttcatgag tgtgttcatg
    13321 ttcagaggat atgttttttt cttgtgaaag agaattcagc tttttattta aatcttcaat
    13381 tttagaaagt actttaaatt gttggtcgta acgcttctca tcagcatttt gtaataattt
    13441 ttcgttgctc gtaataaacc gtaggaattt tttaaaccag ctgacttcct tgggtttggg
    13501 aatctcatct tttttcaata tatctcttac tccagtaata aaagaattga tagcttcatc
    13561 ataattttct ctagctttgc aaatttttgt tgcctctgga agcatattat aatcttcatt
    13621 ttttggatcc ataacatcaa atacagttcc caatttatct ttcatgcttc tgcagttagg
    13681 tatttcaacg cctgctgtat tcgcttcatt aagtaatttc aagagaccac gtgacattat
    13741 tagagtatct agaatatcgg ctggcactaa ttctctagat tgttcaggta aaccttgcaa
    13801 agttactttt tcggcaagct ttcccagggc aggcaaaact gtattatata agttatcttt
    13861 cagattttgg tcgtcggtat tttgatatat cctttgccca tagcccaata ttacagcctg
    13921 gacgaattgc tgagcaatcg ctcgttcatc accctgagca ttttttaaaa ttgctttata
    13981 attttctggc cctatcgcct caataacgtc tggatgaagt gcggttctat cttcattaag
    14041 taaaacaggg attatcccct ttagattttc ctgaatatgc tctcttaatc cattaggttt
    14101 ttgataaatg gtgtttatct cctcatagtt tggagtcggg caatcatact catcaaatat
    14161 tcgtgacaat gctcctatcg aactttgagc gtcctttatt attttactgg gttgattggc
    14221 attaccgttg gggtcatatt cttttttcag atgttcgcgt gaatgcttat taaaaaactt
    14281 aatataagaa tcaccttttt catcagatgg actacttatc tgttgttttt tattctgttg
    14341 agcattaaga atagcccata attgagcttc tgacatatca aattttttta cctctttaga
    14401 tggactattt gtatgttgtt ttttattttg ttgagcatta agaatcgtcc acaattgaga
    14461 ttgcgacaca tcaaatttag ccatgataat ttctccaaaa ttcaaattgt ataaaattag
    14521 aacaattaca gtttaattac cactcattaa ggttaagtta tatccatatc aaataattga
    14581 ataaggcatc aataaagctg ataagtaatg aaccatggta ttgaccatga acgaggttct
    14641 gtatcgcata ttcataacaa tgctgaaggt gtttaatgag tatttgaagg ttaagttttt
    14701 gatagggctt tttaaaacga tagaaaggag ttaaacccaa ttcgttttgc ttgaggataa
    14761 caccaaaatg ctacctgaat atctgctttg tttacccaaa gttcttgggt atggtctgaa
    14821 tatcgctttg cgcaatacct ccgcgccaac aatccaattg cgaaagggta ttatcatgct
    14881 gcgatatcaa tgttatgcat gggggatagg ggatattaat acttacaaaa tgtttaatta
    14941 ataatcattt tgtaatatgg aagcataata ctctaaatag gagaatatta tgccacatag
    15001 tactaaccaa tctaatgtaa acatcaatca atctaatgtt atcgaaaaca tcaatcaata
    15061 cctgaaatgg cataatttgc cagtaagatt tggtactggc ggagtttgta atggattgtc
    15121 tactgttcat gcccaatacg ttttgcaagg tagagaaaga gagtttttca aactgttgcg
    15181 atatgtcgcg ggggataagg gtattctaga cgcggatgat tccgttaaag agaaggttaa
    15241 tgattttgtt tggaaggttg ttgcgagtca tatgacgtca gggcatgata aagaactcaa
    15301 tcaattgaat tcctttaaga cactctctgt taataataaa ccattaaaat ctgtatttga
    15361 tttacctctt gttactagtg atgaaaattg gggaacgatt cttaaagacc ttaaattaaa
    15421 agaagatgag gtaatgttag tacgtagtat taatcataca atcagtatta ctcgcaaagg
    15481 caatcaatac catgtttatg atcccaatta tgaaaaagaa gcggaaccat tttcctctga
    15541 acaggaagtg attaaagagc tgcatgaaag agtatttcat tataataaag gaaatatgga
    15601 taataaagga aatatggggt taatactatc cataatcacc ccagaggata aagagccgag
    15661 acaggacctg ccaaaacctg ttgatattta tggtaaatat ttggataaag agaatgttga
    15721 tagttcagct accatcacgg ttgagcatcc attgggaaaa tgggatttaa ctacaaaaac
    15781 tatagatttg gcatgtgaat gtggtgatga aaatctgatt aaaaaattaa ttgaattaca
    15841 tcagttcgat gttaaaaata ggcctattaa tcaagcatac aaagcggtgc aatacaataa
    15901 tgtaagaaca gctgaattat tagtaaatga catgataaga atattgccga aacaagatca
    15961 agttgaacaa gatgaacttt ctgatatccc cactgaggaa ttaccagaag aattcttaaa
    16021 gcagtttgaa gaaatacctg atgcgccaga gggatatgag attaaagaag cagaagtgca
    16081 aaatccaaaa gtagtgatgt ccccaagtga aaaaaaaatt aaggatttgt tgtaccctct
    16141 tattctaaag gctgcttctt atggaaggaa agaagtattt gatacgctat gggatatacg
    16201 gaatgggtat gacctccatt tgatgtccga attacctcag aatactgaac aatacaaaaa
    16261 cagttatcta tttatcaaaa aaggcgatac tcgggagctg tattatatta agcctgatgg
    16321 aaaagatgaa aaagtagaaa ttaatgattt caatttgttt gaagaaaaaa taaacgccat
    16381 taaaaatgag aatgagatta aattgcgagt cggtgagaag caaattcaag aaattataac
    16441 atcatgcgga ggttcggccc tattgaataa gaatgcagat gattttgata aagaagattt
    16501 aaaagatttt attctatcgg cagcaaaagg aggtaatagt gtgcttttgc aagagcttat
    16561 cgataaatac tatgaaagca ataagacttt atatgataag gatat
//
LOCUS       DATNSN010000067        16522 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.067, whole genome shotgun sequence.
ACCESSION   DATNSN010000067 DATNSN010000000
VERSION     DATNSN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.067"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(93..1418)
                     /locus_tag="VAH76_001712"
     CDS             complement(93..1418)
                     /locus_tag="VAH76_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HEO1386334.1"
                     /translation="MWTHRRSGQTNQRGSQDHRDPYERDRTRVIHCPAFRRLQRKTQI
                     LGTDEGDFHRTRLTHSLEVDSIGRSIVRNLMTNQEHQLLLSSLLPNDDLISVICLLHD
                     IGHPPFGHGGEVALNYMMRHYGGFEGNGQTLRLLTKVENSYGGFGLDLTRRALLGILK
                     YPVKRANVVAPQQPPIHESIHKTIKINDWLPPKAYFDCEQPEVDWLLSPLSDNDRELF
                     QSLSVKPNGTQAGKAAYHNFDCSIMDTADDIAYGVHDLEDAIHLRLINRSHLDTPEFR
                     QLISETTLSLHRERLLNSLFSPEICLRKQAIGEMVNYFITSTQIIVTNEEFENSLLKH
                     NIALIPEAHALLSYLMQCIYNNVIDSQEARTFEYGGQTVVLRLFDAISSNPASLLDNK
                     NRVLFSQAPDEVAAYRVVCDYLANMTDEYAYRMHERLFGFNTRTIFERL"
     gene            1694..2227
                     /locus_tag="VAH76_001713"
     CDS             1694..2227
                     /locus_tag="VAH76_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEO1386335.1"
                     /translation="MMGISIRYIILGISVMNISSCMVYEDTYTTRYQIYTYDYGQLYP
                     QADYHARDYNYGNQSGQGVVVPDSYHVGEYHSPVSFKDRDRNWVNSQNPQGYTIQIAD
                     DEKASYVAQKLYKAPKNDRMAQVKYYQNGKTHYKGLYGTYDSQEAAQKALESLPPEIK
                     QGAGIKDWGSIQGNLNE"
     gene            complement(2331..2801)
                     /gene="lspM"
                     /locus_tag="VAH76_001714"
                     /gene_synonym="gspM"
     CDS             complement(2331..2801)
                     /gene="lspM"
                     /locus_tag="VAH76_001714"
                     /gene_synonym="gspM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     LspM"
                     /protein_id="HEO1386336.1"
                     /translation="MKTYLNTLNDREKWMVIVAGVSLFIYGYYLLLYAPLSNQVNQKS
                     TQLIEKTETLEWMKQVRMQKRSAKRKESVDNSQLLTILASQLKNNKTLKFPYQLQQTG
                     SGDVQLTFDEVPFQNFIQWLAKINEVYSINIKQFDVEKTPTPGVTRLMIILSTS"
     gene            complement(2798..3937)
                     /gene="lspL"
                     /locus_tag="VAH76_001715"
                     /gene_synonym="gspL"
     CDS             complement(2798..3937)
                     /gene="lspL"
                     /locus_tag="VAH76_001715"
                     /gene_synonym="gspL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL family type II secretion system protein
                     LspL"
                     /protein_id="HEO1386337.1"
                     /translation="MDKCFIFSKHLDDNGCVCLKISDLGELIAPPEFRNFSQIKDLQK
                     ESSTIIVETCTRAILLGLALPWLPERKARIAIPYALEDKVTQPVEELHFAFDKFYYQN
                     NQYLVVVIDKQRMRKLIQVFDEHGIEFDAVTLDWFALASQELIISETELLINNEDFKG
                     VLSGDLAQTYLKNHPHNPAHLFQDSKIPVASPASQNEEHSYTWISKRLIKSRPLNLCQ
                     GEIQHGKTTDWVKKGYLLVGGLCGFWLISILFVNWLSLHWLNRQIDEIDQQTAVIYRE
                     FFPDAKQVISPKFRISQLLGGSTAENQTRFWFILNQLSKAIKDSGITVEQLRYQNKTI
                     SITLVSSDFESLQKIENKLKQSQLKVKQTQASTKDQQVVATLELT"
     gene            complement(4012..5028)
                     /gene="mltB"
                     /locus_tag="VAH76_001716"
     CDS             complement(4012..5028)
                     /gene="mltB"
                     /locus_tag="VAH76_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEO1386338.1"
                     /translation="MRQIIWLGFISLFLLSFTTYSDTTFTKRKDVQSFINMMVTNYHF
                     NRNQLTTVMNQVKLQPQVIESMEKPYEKKNWDVYRELFLTQKRLKSGLQFWAENQKTL
                     EKIQRRYGVSPEIIIAILGVETMYGERQGEYRVLDTLATLAFNYPKRSAFFTKELKEF
                     FLLCREQHVSPTQYKGSYAGAIGKPQFMPSSYRYYAVDFNNNGRRDLVNDNGDSIASI
                     ANYLHKHGWNLNQGIAQPAKISGWRYKQLRMNPKTANYRYSQLISYGVKPITASQNHP
                     PRAGVIELVTRKGKEYWIAYPNFFVITRYNSSPQYALAVYLLSQQLKQHWYTSATKRH
                     RAYV"
     gene            5223..7055
                     /gene="lepA"
                     /locus_tag="VAH76_001717"
     CDS             5223..7055
                     /gene="lepA"
                     /locus_tag="VAH76_001717"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO1386339.1"
                     /translation="MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLT
                     EREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSR
                     SLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
                     DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGV
                     VSLVRIVNGAIRKGDKMRVMSTGRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKE
                     IQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDA
                     SLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGE
                     TLLIDNPSHLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRH
                     VSVVYDIPMSEVVSDFFDRLKSVSRGYASLDYNFQRFQIADLVKMDILINSERVDALA
                     VIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKNVTAKCY
                     GGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK"
     gene            7163..7918
                     /gene="lepB"
                     /locus_tag="VAH76_001718"
     CDS             7163..7918
                     /gene="lepB"
                     /locus_tag="VAH76_001718"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO1386340.1"
                     /translation="MNFALILVILSAVTGLIALLDWLIWSKKRLPEQKPGRIIEYSRS
                     FFPVFFLVLLLRSFLIEPFRIPSGSLEPTLLVGDFVAVNKFSYGLRLPVWEKKIVPIA
                     NPKTGEIAVFRWPPEPSYDYIKRVIGVPGDKISYHDKKLTINGKEVKQTFVEYTTDES
                     SGKAVTKYKEDLNGVVHDIFVRPDAPAVDFDIVVPEGNYFMMGDNRDDSADSRFWGFV
                     PDSYLRGKAFLVWMSWNGKTDNVRWSRIGKLIH"
     gene            7930..8322
                     /locus_tag="VAH76_001719"
     CDS             7930..8322
                     /locus_tag="VAH76_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEO1386341.1"
                     /translation="MRKQQGMTLIGMLFTAVVVIMAGILVMRAAPVYIQHYSILHSIK
                     ALNSTPVSSLSGDPLTDVTTLRESVTKRLDINGVEDLKPGELVITPSGPNKFKIKLKY
                     QVIKPLIYNMSLLFNFEDNFEVITGSEN"
     gene            8312..8986
                     /gene="rnc"
                     /locus_tag="VAH76_001720"
     CDS             8312..8986
                     /gene="rnc"
                     /locus_tag="VAH76_001720"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO1386342.1"
                     /translation="MKINLERLCRRLNYHFNNIAYLKQALTHCSAGSDNYERFEFLGD
                     SILSFVIANELFNRFPLHSEGQLSRLRSFLVKGEMLAEIAREIGLGDYLFLGQGELRS
                     GGFRRTSILADALEAILAAIYLDGGMTAAKQIILMLYSSRLDDPDLNHCLKDAKTQLQ
                     EFLQASKFALPEYVLTKIEGDEHAQIFHVTCTIEGVSQVAYGTGPNRRKAEQLAAKAM
                     LEQLQG"
     gene            complement(9014..10864)
                     /locus_tag="VAH76_001721"
     CDS             complement(9014..10864)
                     /locus_tag="VAH76_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO1386343.1"
                     /translation="MLTLRQITLSRGDKILLDKVNVSLYEKQKIGLIGHNGCGKSTLF
                     DFLLGQLSPDTGEYLINPQLSISHLSQHIPDSNEKALDFVLAGDQQYIKLLKRLSKAE
                     KTGDDAEVLQCHEELSHTGGYSKPAQAAVIMSGLGFSGNQQQEPVNSFSGGWRMRLNL
                     ARCLMKPADLLLLDEPTNHLDMEAIFWLERYLKQSPSTIILISHDREFLDAFVTHILH
                     IENRNLTLYSGNYSSFEKIHAQQLALQQAMHEKQQTKIKHMMTFVTRFKAKATKAKQA
                     QSRMKAIEKMEIIAAAQADSPFSFDFFPCPRAGNPLIQCNQVDAGYINHPPILKKIKL
                     SLNPGDRIALLGPNGEGKSTLIKTLTADLPVLSGDIHRSPHLQIGYYAQHQLEQLDCK
                     LSPVETLQTLSPEVREQTIRDFLGGFNFRGDMAVQSIQHFSGGEKARLALARLVWLKP
                     NLLLLDEPTNHLDLGMRSAIELALQTYEGALILISHDRHLLKSSVDNFYLIYQGKVQP
                     FEGDLDDYYLWLQTRNSEKESKLATSPNEYKERKSYQNKLKKLEQLIEHYQNQLRLLD
                     KQLADVSLYEDTKQKSKLDNLLMERDIAHSALIKTEEEWLEICHALESLE"
     gene            complement(10986..12212)
                     /locus_tag="VAH76_001722"
     CDS             complement(10986..12212)
                     /locus_tag="VAH76_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO1386344.1"
                     /translation="MIMKTKLLPLFDNLNHIHKNHENVILPDARFQNDFTYCLSFLKS
                     YIGSEGTFNSYRRETERLLQWSWLVKNSTIDSLRRDDIEQYIHFCQNPPKTWISLKKV
                     PRFIEKNGKRIPNEEWRPFVVTLSKVSRKNGHLASIDQFKLSQGAVQEIFAILSTLFN
                     FLIAEEYLISNPVALIRQKSKFIRKRQHNAPVRRLSLVQWEAVLRAAESLAEDSPEKH
                     ERTRFILSLLFGMYLRISELAASERWVPSMNDFAKDSNGHWWFTTVGKGNKERQIAVS
                     DAMLESLTRWRLFLGLTPLPSPADNSPLLPKLRGHGPMSDTAPIRRIVQHCFDLAEEQ
                     LRLNGLSEEADNLSAATVHWLRHTGISEDVKIRPREHVRDDAGHSSSATTDRYIDIEK
                     QARYQSARKKIIQPSE"
     gene            12484..12560
                     /locus_tag="VAH76_001723"
     tRNA            12484..12560
                     /locus_tag="VAH76_001723"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12518..12520,aa:Pro,seq:tgg)
     gene            12566..12642
                     /locus_tag="VAH76_001724"
     tRNA            12566..12642
                     /locus_tag="VAH76_001724"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12600..12602,aa:Arg,seq:tct)
     gene            12803..12878
                     /locus_tag="VAH76_001725"
     tRNA            12803..12878
                     /locus_tag="VAH76_001725"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12836..12838,aa:His,seq:gtg)
     gene            12969..13053
                     /locus_tag="VAH76_001726"
     tRNA            12969..13053
                     /locus_tag="VAH76_001726"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13003..13005,aa:Leu,seq:tag)
     gene            13083..14414
                     /locus_tag="VAH76_001727"
     CDS             13083..14414
                     /locus_tag="VAH76_001727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO1386345.1"
                     /translation="MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGF
                     RPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKD
                     FKYTAVFEVMPVFEIVELNQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
                     GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFP
                     EDYGHKDLAGKEATFKITIKKIMEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMAR
                     ELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIP
                     DFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWY
                     QSSKEHMAEVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGE"
     gene            14417..15060
                     /gene="clpP"
                     /locus_tag="VAH76_001728"
                     /pseudo
     CDS             14417..15060
                     /gene="clpP"
                     /locus_tag="VAH76_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
     gene            15201..16475
                     /gene="clpX"
                     /locus_tag="VAH76_001729"
     CDS             15201..16475
                     /gene="clpX"
                     /locus_tag="VAH76_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO1386346.1"
                     /translation="MSKSGNGNGDKVLYCSFCGKSQHEVKKLIAGPSVFVCDECVELC
                     NDIIREETHETHEETEARLPTPKEISNFLDEYVIGQQHAKKVLSVAVYNHYKRLQHKS
                     EDGVELGKSNILLIGPTGSGKTLLAQTLARILNVPFAMADATTLTEAGYVGEDVENII
                     QKLLQKCDYDVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVA
                     SVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRERSDKSSIGFSAQLKSKKS
                     SNDEVSKVLGQLESDDLIKYGLIPEFVGRLPVVTTLQELDEAALIDILTRPKNALTKQ
                     FQSLFKMEGSELEFRDEALIAIAKKALERKMGARGLRSILENILLDTMYDLPSLEGVN
                     KVVIDESVVNGLSKPILIYEQDEKKSASGSKD"
BASE COUNT         4895 a         2842 c         3513 g         5272 t
ORIGIN      
        1 ccccaataat aattatattt ttagttgaaa ggtttttatt aagtaacaat ttggttttgt
       61 tttaatgtca ttactttaca tgtttgccat ttttaaagcc tttcaaaaat tgttcttgta
      121 ttgaacccga ataatctctc atgcattcga taagcatact cgtcagtcat gtttgctaga
      181 taatcacaaa cgactctata agcagcgacc tcatctggag cttgcgaaaa taacactcga
      241 tttttattat caagcaagct ggctggattg gagctgatgg catcaaatag ccggagtact
      301 accgtttgac ctccatattc aaaggtacgt gcttcttggg agtcaatgac attattatag
      361 atacattgca ttaaatagct gaggagggca tgggcttctg ggataagtgc aatattatgt
      421 tttaataaac tgttttcaaa ttcttcattg gttacaatga tttgagtaga ggtaataaaa
      481 taattgacca tttctccaat agcttgtttt cttaaacaga tttctggaga aaatagagaa
      541 ttaagcaaac gctcccgatg tagcgagagg gttgtttcac tgattaattg cctgaactca
      601 ggagtatcaa gatgtgaacg gttaattaat cttaagtgaa ttgcatcttc caggtcatgg
      661 actccatagg caatatcgtc cgctgtgtcc ataatagagc aatcaaaatt atgatatgct
      721 gctttacctg cttgggtgcc attgggcttt acggagagag actgaaataa ttctctgtca
      781 ttatctgata aaggcgataa taaccaatcg acttctggct gctcgcaatc aaaataagct
      841 ttaggaggca accagtcatt gattttgatt gttttatgaa tcgattcatg aatagggggt
      901 tgttgtggag ccaccacatt agctcgtttt accgggtatt ttaagatacc tagcagcgca
      961 cgacgagtta gatccaggcc aaaaccaccg taactatttt caacttttgt cagtaagcgt
     1021 agagtttgtc cattgccttc aaatcctcca taatgtcgca tcatataatt caatgcgact
     1081 tctcctccat gtccgaaggg cggatggcca atgtcatgaa gcaaacaaat gacgctgatg
     1141 agatcatcat ttggcaaaag gctggacaat aacagctggt gttcctgatt ggtcatcaag
     1201 ttgcgaacga tacttctacc tatagaatct acttccaggg agtgggttag gcgggttcta
     1261 tgaaaatcgc cctcatctgt tccgagaatt tgtgtttttc tttgtagtct tctaaatgct
     1321 ggacagtgaa ttactcttgt cctatctcgc tcatagggat ctctatgatc ttgagagcct
     1381 ctttgattgg tttgaccaga acgtctatga gtccacatat cttttgccct tattccacaa
     1441 actaattact ttattctata ccatgttggg aaacaataga gtctttgcat agtctctgga
     1501 aaatcacgat tgtttataat gacttttatt tttttgttat atgcagaata agagaaattt
     1561 tttcggacga tggcttgaat agaaatccaa taactctgaa tcaactcata aaactttttc
     1621 ctaaagtttt ccttattttt gtagataact acaaataaga ataaaagtaa aagttaaaat
     1681 cagggggggt gttatgatgg gtattagcat caggtacatc attctaggta tcagcgtaat
     1741 gaatatttct tcttgtatgg tttatgagga tacatacact acccgctatc aaatttatac
     1801 ctatgattat ggtcagttgt atcctcaagc agattatcat gctcgtgatt acaattatgg
     1861 caatcaatct ggtcaaggtg ttgtggttcc tgattcatat catgttggtg agtatcattc
     1921 tcctgtatcc tttaaggaca gagatagaaa ttgggtaaac agtcaaaatc cccaaggcta
     1981 tacgattcag attgccgatg acgaaaaggc ttcttatgtg gcccaaaaac tctacaaagc
     2041 acctaaaaat gatagaatgg ctcaagtgaa atattatcaa aatggcaaaa ctcattataa
     2101 gggtttatat ggaacttatg atagtcaaga agcagctcaa aaagcattgg aatcattacc
     2161 ccctgaaatt aaacagggtg ctggaattaa agattggggg agtatccaag gaaatttgaa
     2221 tgaataggtt atcttgagaa actatatgta tttaaaaaat actttgatat tctgtatttt
     2281 attattctaa ggtcaggact tatagtcttg actaatacat aatagtactt ttaacttgta
     2341 gagagtatga tcatcagtcg ggttactccc ggtgttgggg ttttctccac atcaaattgt
     2401 tttatattga tagaatagac ttcgttgatt tttgcaagcc attgtataaa gttttgaaaa
     2461 gggacttcgt caaaggttaa ttgaacatcc cccgagcctg tttgttgtaa ttgatagggg
     2521 aatttgagtg ttttattatt tttaagttgg gaagcaagaa tagttaacaa ttggctgtta
     2581 tctacagatt cctttctttt ggcagagcgt ttttgcattc ttacttgctt catccattcc
     2641 aatgtttcag ttttttcaat taattgagtg gatttttgat ttacctggtt gctcagaggg
     2701 gcataaagta ataaatagta gccataaata aaaagtgata caccagccac tataaccatc
     2761 cacttttctc gatcattcag cgtgtttaaa taggttttca cgttagctcc agagttgcaa
     2821 ccacttgttg atctttcgtt gaggcttgag tttgtttcac tttgagttgt gattgtttta
     2881 gtttattttc aattttttgt aagctttcaa aatcagaact tactaatgtg attgagattg
     2941 ttttgttttg ataccttaat tgctctactg tgataccact atcttttatg gctttggata
     3001 attgatttaa tataaaccaa aatcgagttt gattttctgc agtactcccc ccaagaagct
     3061 gacttattcg aaacttagga ctgatgacct gtttggcatc aggaaaaaac tcacgataaa
     3121 taaccgcggt ttgctggtca atttcatcta tttgtctatt gagccaatgc aaagaaagcc
     3181 aattaacaaa aagaatagaa attaaccaga agccgcataa tccgccaacc aataggtatc
     3241 cttttttaac ccaatcggtt gtttttccat gttgtatttc accttgacaa agattgagtg
     3301 gtcttgattt tatcaatctt ttactaatcc aagtgtatga gtgttcttca ttttggcttg
     3361 ctggagaggc gacaggtatt ttgctgtctt gaaacagatg agcgggattg tgtggatgat
     3421 ttttcaggta agtttgagcc aaatcacctg agaggacacc tttaaaatct tcgttattga
     3481 ttaatagctc ggtttcacta atgatcaatt cttgagatgc tagcgcaaac cagtcaagag
     3541 ttactgcatc aaactctatc ccatgctcat caaaaacttg aattaacttt ctcattcgtt
     3601 gtttatcaat cacaacaacg agatattggt tattctgata ataaaattta tcaaaggcaa
     3661 aatgaagttc ttcgactggt tgagttactt tatcctcaag tgcataggga attgcaattc
     3721 tggcttttct ttcaggtagc catggaagtg caagaccaag aagtattgct ctggtacagg
     3781 tttctacaat aattgtactg gattcttttt gtaaatcttt tatttgcgag aaattacgaa
     3841 attcaggggg agcaatgagc tcacccaagt cagatatttt caggcataca caaccattat
     3901 catcgagatg tttgctaaaa ataaaacact tatccatgag tcaaactctg tattgttatg
     3961 gctgtcaatt tttcagtata taccagtaat tattattatt tccagctggc attaaacata
     4021 agccctgtgc cttttagtgg cactagtata ccaatgttgc ttcaattgct gggataataa
     4081 ataaaccgcc agagcatatt gagggctgga gttatagcgg gtaattacaa aaaaattggg
     4141 ataggcaatc cagtattctt tacccttcct tgtaactaac tcaatgactc ctgcacgagg
     4201 aggatgattt tgtgacgctg taattggttt tactccatag cttataagtt gtgagtatcg
     4261 gtaatttgct gttttagggt tcatacgaag ttgtttgtat cgccatccgg agattttggc
     4321 cggttgagcg atgccttgat ttaagttcca accatgctta tgcaaataat tggcgatgct
     4381 ggcaatagag tcaccattgt cattgactaa gtctcttcga ccattgttat taaagtccac
     4441 tgcataatat cgataactgc ttggcataaa ttgaggtttt cctatggcac ctgcgtaaga
     4501 ccctttgtat tgggttgggg atacgtgctg ttctcggcac aggaggaaaa actcctttaa
     4561 ttctttagta aagaatgcag aacgtttggg gtagttgaaa gccagtgtag ccagagtatc
     4621 caagactctg tattcaccct gacgctcacc atacattgtc tctactccta aaatagctat
     4681 aatgatctcc ggagacaccc cataccttct ctgaattttt tccaatgttt tttgattttc
     4741 agcccaaaat tgcaatccgc tttttaatct tttctgagta agaaatagtt ctcggtagac
     4801 atcccagttt ttcttttcat agggcttttc catagattcg atgacttgag gttgaagttt
     4861 aacttggttc attactgtag ttaactgatt acgattaaag tgataattcg ttaccatcat
     4921 gttaataaac gattgtacgt ctttacgttt tgtaaatgtt gtgtcggaat aggtagtgaa
     4981 agataaaagg aagagtgaaa tgaaacctag ccagatgatt tgtcgcatca tgtaccctgt
     5041 atcgttgcaa accacgaatt ttagtaaaaa taaagcctca tagcaaattt ttggcctttg
     5101 ttcatcttaa atacagtgca tgaaattggt aaatgaataa gcaattttca ttctcttaag
     5161 aatataaaaa cgactgaatt tgctttgcct actttctgca cgcgaacatt taagatagac
     5221 ttatgctatt tgcaagaagg ttagaacagt tgaaagattt aaaccgaatt cgaaattttt
     5281 caataattgc ccatattgat catggcaagt ctacgttggc tgatcgattt atccaaattt
     5341 gtggcgggtt gactgagcgt gaaatgagct cgcaagttct tgattctatg gatattgaac
     5401 gtgagcgagg tattaccatc aaagcgcaat gtgtttcctt gaattacaca gctaaagatg
     5461 gaaagaccta tttattgaat tttatcgata cgccagggca tgttgatttt agttacgagg
     5521 tatctcgttc tcttgccgct tgtgaaggtg ctattctggt tgttgatgca gcacagggag
     5581 tagaagcgca aactttagca gtttgttata cagcaattga tcagtctctt accgtattgc
     5641 cagtactgaa taaaattgat ttaccgcagg cagaacctga acgggttatt tccgagatcg
     5701 aagatattat tggactcgat gctcaagatg cgattagagt gagtgctaaa agcggcttgg
     5761 gtgttaatga tgttttagag gcattagttg cgaatattcc ccctccgaaa ggcgatgttc
     5821 atgccccatt acaagcactt attattgact cctggtttga tagttattta ggagttgtct
     5881 cactggttcg tattgttaat ggcgccatac gaaaagggga taaaatgcga gtgatgtcca
     5941 caggcagggc ttatgaagtg gatcaagttg gtatttttac ccccaaacgg actaaactgg
     6001 atgctttata tgctggtgaa gtgggttatg ttgttgctgg tataaaggaa attcagggcg
     6061 ctccagttgg tgatacttta actctggata gaaatccagc tgataaagtt cttccagggt
     6121 ttcaacgcgt taagccccaa gtttatgctg gtttgtttcc agtcagctcg gatgattttg
     6181 aggcatttag agaggctttg gctaaattga gtttaaatga tgcttcttta ttttatgagc
     6241 cggaatcttc agaggctttg ggatttggtt ttcgttgcgg atttttaggc atgctgcata
     6301 tggaaatcat tcaggagaga ttggaaagag aatataacct tgatttgatt tctacggcac
     6361 ctactgttgt ttatcagatt gtaactcaga aaggggaaac tttattaatt gataatcctt
     6421 cgcatttgcc cccaaccccg caaataaaag agatgtatga gccaattgtc agagcaaata
     6481 ttctagtgcc acaagattat ttggggccta tcattacctt atgtgttgaa agacgtggtg
     6541 ttcaagtcag tatgacctat agtggtcgtc atgtttctgt tgtttatgat attcccatga
     6601 gcgaagtagt ctctgatttc tttgatagat taaaatcagt tagtagaggt tatgcctctt
     6661 tggattataa tttccaaaga ttccagattg ctgatttggt caagatggat atattaataa
     6721 atagcgaacg tgttgatgct cttgctgtga ttgttcatag agattccgct catagccgcg
     6781 gaaaattaat tgcggagaag atgcaacagc ttattccaag acaaatgttt gatgtggcta
     6841 ttcaggctgc tataggaagc catattattg ctaggcaaac agtgaaagca ttgcgcaaaa
     6901 acgttacagc aaagtgttat ggtggtgatg tgacgcgtaa gcggaaattg ctggaaaaac
     6961 aaaaagctgg caagaaaaga atgaaacagg tggggcatgt agaaatacct caagaagcat
     7021 ttatggccgt gtttcaaaca gataaaaaaa agtagagccg ctcatttact aataaggatg
     7081 tgttgacctt ttcgtcagca tctggacata agtcaaatca tgattaaatt gtcttttgct
     7141 taaaattgag gatattgaat ttatgaattt tgctttaata ttggtcatat tatccgctgt
     7201 aacaggattg attgctttat tggattggtt gatttggtct aaaaaacgtt tgcctgaaca
     7261 aaagcctggt cgaatcattg agtattcacg ttcttttttt cctgtatttt ttcttgttct
     7321 gctattgcga tcttttttaa ttgaaccttt tcgtattcca tcgggctcac ttgagcctac
     7381 tttgttggtt ggtgattttg tagcggttaa taaattttct tatggactga gacttccagt
     7441 atgggagaaa aaaatagtgc caattgctaa tcccaaaacg ggagaaattg cagtatttcg
     7501 ctggccacct gaaccatctt atgactatat taaaagagtt attggcgtgc ctggggataa
     7561 aatttcatat catgataaaa aactaactat taatggcaaa gaggtgaaac agacttttgt
     7621 tgagtatact accgatgaaa gctctggcaa ggctgtaaca aagtataaag aagatttaaa
     7681 cggtgtggtg cacgacatat ttgtcagacc tgatgcgcca gccgtagatt ttgatatagt
     7741 tgttcctgag gggaattact ttatgatggg agataatcgg gatgatagcg ccgatagtcg
     7801 gttctgggga tttgtacctg atagctattt aagaggaaaa gcatttctgg tctggatgag
     7861 ttggaatggc aaaacagata atgtacgctg gtcaaggata ggcaaattaa ttcattgagg
     7921 gaatagggca tgagaaaaca acagggaatg acattgattg gtatgctgtt tactgccgta
     7981 gtggtgataa tggctggtat acttgttatg cgggcagcgc cagtttatat tcaacattat
     8041 tcgattttgc attcaatcaa agcattaaat tcaacacctg tttcttcttt gtcgggggat
     8101 cccttgaccg atgttacaac tttaagggaa agtgtgacta aacgattaga tatcaatggg
     8161 gtagaggacc taaaaccagg agagctggtt attactcctt caggccccaa taaatttaaa
     8221 attaagttaa aatatcaagt catcaaacct ctaatttata atatgagctt attatttaat
     8281 tttgaagata attttgaggt aataacaggt agtgaaaatt aatttagaaa gattgtgcag
     8341 acgtttaaac tatcatttta ataacatagc ctatttgaag caggctttga cgcattgcag
     8401 tgctggtagc gataattatg aacgctttga gtttcttgga gactcgatat taagttttgt
     8461 tattgccaat gagctattca atcgttttcc acttcacagt gaagggcaat taagtcgatt
     8521 gcgctctttt cttgttaaag gtgaaatgtt agcagaaata gccagagaaa taggtttggg
     8581 tgattatcta tttcttgggc aaggtgagtt aaggagcggt ggatttcgcc gcacatctat
     8641 attagcagat gcccttgagg ctatattggc agccatttat ttggatggag gaatgactgc
     8701 tgcaaaacag attattttaa tgctttatag ttcacgatta gatgatccgg atttaaatca
     8761 ttgtttaaag gatgcaaaga cacaattaca ggagtttttg caagcatcaa aattcgcttt
     8821 acctgaatac gtcctaacca agattgaggg ggatgagcac gctcaaatct tccatgtaac
     8881 atgcacaatt gagggagtaa gtcaggtagc ttatggcaca gggcctaata gaagaaaagc
     8941 tgagcagttg gcagcaaaag ccatgttgga acagttacaa ggttaaatgg tattgatcag
     9001 cagaacctat tacttactct aggctttcca gggcatgaca tatttctaac cactcttctt
     9061 ctgtcttaat taaagcggaa tgagcgatat ctcgttccat caaaagatta tccaatttgc
     9121 ttttctgctt tgtgtcttca tacagagaaa cgtcagctaa ctgcttatca agaaggcgca
     9181 attgattctg atagtgctca atgagttgct ccagcttttt taatttgttt tgatagcttt
     9241 ttctttcttt atattcattt ggggaagtag ctaatttgga ttccttttct gaatttctgg
     9301 tttgcagcca gagatagtaa tcatcaagat ccccttcaaa tggttgtact tttccttgat
     9361 aaataagata aaaattgtcc acgcttgatt ttaacaaatg gcggtcatgc gaaatcaaaa
     9421 ttaaggcgcc ctcataagtt tgcagcgcta attcaattgc tgagcgcatt ccaagatcca
     9481 aatggtttgt tggctcatcc agcaaaagaa ggttgggttt tagccaaacc agtcttgcta
     9541 atgccaggcg ggctttttcg ccacctgaaa agtgctgaat ggattgtacc gccatatccc
     9601 ctctgaaatt aaatccacct aaaaaatccc gtatagtttg ttctcttacc tctggcgaga
     9661 gagtttgcag tgtttctacc gggcttaatt tacagtctaa ttgctcaagt tgatgctgag
     9721 catagtagcc tatttgtaaa tgcggggaac gatggatatc tccacttaat acgggcaaat
     9781 ctgccgtaag cgttttaatc aaggttgatt ttccttcacc gttaggtccc agtagcgcaa
     9841 ttctatcgcc gggattaaga gagagtttga tttttttcaa aatcggggga tgattgatat
     9901 atcctgcatc aacttgattg cattgaatta aaggatttcc tgcccgagga caagggaaaa
     9961 aatcaaaaga aaaaggggag tcggcttggg ctgctgcgat tatttccatc ttttctatag
    10021 ctttcattcg actttgcgct tgtttggcct tggttgcttt cgctttaaaa cgagttacaa
    10081 aagtcatcat atgtttgatt ttggtttgtt gcttttcatg cattgcttgt tgtagagcca
    10141 attgttgcgc atgaattttt tcaaagctgc tgtagtttcc tgaataaagt gtcagattac
    10201 gattttctat atgtaatata tgagtaacaa aggcatcaag aaattctcta tcgtgtgaaa
    10261 taagaataat agtacttggg ctttgtttta aatagcgttc cagccagaag atggcttcca
    10321 tgtctaaatg gtttgtgggt tcatcaagca ggagtaaatc ggcgggtttc attaagcacc
    10381 gagcaaggtt taagcgcatt ctccagccac cagaaaagct attaacgggc tcttgctgtt
    10441 gatttccact aaatcctaag ccagacataa taactgctgc ttgcgcgggc ttactgtaac
    10501 caccagtatg agagagttcc tcgtgacatt gcaaaacttc tgcatcatcg ccggtttttt
    10561 cagcttttga aaggcgcttt agcaatttaa tgtattgttg atctccggca agaacaaaat
    10621 ccaaggcttt ttcattgcta tcaggtatgt gttgagacaa atgactgatg ctgagttgtg
    10681 gattaattag atattctcca gtatcaggag ataattgacc taaaagaaaa tcaaataatg
    10741 ttgattttcc acatccatta tgaccgataa ggccaatttt ttgtttttca taaagactga
    10801 cattaacctt atcgagtaaa attttatctc ctctggataa agtaatttga cgcaatgtaa
    10861 gcatgttaga ccgtaagctt gaactaatca gccattttat cataaattag cttaagcttc
    10921 ctattgatca agtaagcgag ttggatattg cataagttca aaaccggatt ttctttaaaa
    10981 ataagttatt cgctgggctg aattattttc tttcgcgccg attgataacg tgcttgcttt
    11041 tctatatcaa tatacctgtc cgttgtagca cttgaactgt gcccggcatc atctcgcaca
    11101 tgctctctgg gcctgatttt tacatcttct gatataccag tatgccgcag ccaatgcacc
    11161 gtggcagcgg atagattatc cgcttcctct gataaaccat taagtcgcag ttgttcttct
    11221 gccaagtcaa aacaatgctg aacaattcgc cttataggag ctgtgtcact cattggacca
    11281 tgaccacgca gcttaggtag taaaggcgaa ttatcagctg gtgatggaag tggggtaagt
    11341 ccaagaaata atcgccagcg ggtaaggctt tccagcatag cgtcgcttac cgcaatttgt
    11401 cgttccttat ttccttttcc tacggtggta aaccaccagt ggccattact gtccttggca
    11461 aaatcattca tactgggtac ccatctctca ctggcagcaa gttctgatat acgaagatac
    11521 atgccaaata acagacttaa aataaaacga gtgcgttcat gtttttcagg ggaatcttca
    11581 gccaaacttt cagctgctct caggacagct tcccattgaa ccagacttaa tcgtctaaca
    11641 ggcgcgttat gctgtctttt gcgaataaat ttgctttttt gcctaataag ggcaactgga
    11701 ttggatatca gatattcttc agcaattaga aagttgaata gagtacttaa gatggcgaat
    11761 atttcttgca cggctccctg agataacttg aattgatcta ttgatgcaag atgcccattt
    11821 ttcctggaaa ccttacttag tgtcactaca aaaggtcgcc actcttcatt aggtatccgt
    11881 ttaccatttt tttctataaa tcgtggaact ttttttagac taatccatgt tttaggcggg
    11941 ttttgacaaa aatggatgta ttgttcaata tcgtcacgcc ttaaagaatc aatagtagag
    12001 tttttgacaa gccatgacca ctgtaacaaa cgttctgttt ctcttctgta actgttgaat
    12061 gtgccttcgc ttcctatata gctttttaaa aaacttaagc aataagtgaa atcattctga
    12121 aatcgcgcat caggtaatat cacattttca tgatttttat ggatgtgatt gagattatca
    12181 aacaagggta atagttttgt tttcatgatc ataacttatc tgctgatatg tcattatgct
    12241 aacctaattg ctgatttaag tcatgaggtt gatagtgcat tagtataagg gcatgcgtta
    12301 ttgtaaatta tagtgttggg gcagctacgt aagtgcctgt aaagggcaat aaattgacta
    12361 aaagtacgga agaaattcag attggaattc agactatata ataaataaaa attatatgct
    12421 aaaggatatt gaccttcgtg atagcaccta gtattatctg cgctctgtcc ttgggacggg
    12481 tatcggggtg tagcgcagtc tggtagcgcg cctgctttgg gagcaggatg tcgggggttc
    12541 aaatccctcc tccccgacca tttaagcgcc cgtagctcat ctggatagag catcggcctt
    12601 ctaagccgag ggtagcaggt tcgaatcctg ccgggcgcgc cagccgcctt gcttttgcag
    12661 tattatcgct ggaacagggc ttacgaaacc ctccatggtt gcataatttt taataaaaac
    12721 ctattttgtg caagtagcta tattgaaaat ttatgaacgt ataatttggg aagtttcgta
    12781 agccctgtta agagtaattt tggtgagcgt agctcagttg gtagagcccc gggttgtgat
    12841 cccggttgtc gtgggttcga gtcccatcgt tcaccccatt tgtaacagat ataattttga
    12901 gtctaccgct aaagattcca tctgagtctt tacaagactg aattactgat tgataatcca
    12961 acctgattgc gaaagtggcg gaattggtag acgcactgga tttaggttcc agcggggcga
    13021 cccgtgagag ttcgagtctc tcctttcgca ccatttcatg ataacttccg agaggtgaat
    13081 taatgcaagt ttctgttgag accctagaag gtttggagcg taaggttaca gtttctgtac
    13141 ctacagagaa agttgaggaa gaggtgagct cgcgtctcag aaatcttgct cgcaaagtga
    13201 aaatagatgg ttttcgtcca ggcaaagtcc cttttaatgt tgtaaaaagc cgtttctctg
    13261 atagtgtgcg tgaagaagtt gcccgggaaa tggtacaatc taccttatat gaagccttac
    13321 aaaaaaatga attggtgcca gcaggttatc ctcatgttga gcctttagaa atcgaaccag
    13381 gtaaggattt taaatacact gctgtatttg aagttatgcc cgtttttgaa attgtcgaac
    13441 tcaatcaagc tccagtggaa ttaattcaat ctgaagtcac tgataaagat gttgacaaca
    13501 tgattgagaa attacgagaa caaaacaaag agtggcatga agtaactcat gcggtcaaaa
    13561 aaggtgataa agttgttatt gattttcagg gatttttgga tgacaagcca ttccagggcg
    13621 gcagtgctga aggatatgag cttgtgattg gttctggatc gatgattccc ggatttgaag
    13681 atggaattgt tggtgggaaa attgacaaac cgttcgatat aaaagtgagt ttcccggaag
    13741 attatggcca taaagatttg gctgggaagg aagcaacgtt caagatcact atcaaaaaga
    13801 ttatggaagg caagttgcct gctttggatg aggcatttgc ggagaaattt aatataaaag
    13861 aaggtggtat agagtctctt aaaaaggata ttagagaaaa tatggcgcgt gaattggaac
    13921 gtcgcgtcaa catgatgaac agagagaaac tgtttgattc attaatgtct gtgaatcatg
    13981 ttgagttgcc aatagctttg atagataaag aaattgagca tttaaagcat gatatgtatc
    14041 accgattgtt tggtcacgag cataaagacg atgaaaaaat tcctgatttc ccaagggagc
    14101 tatttgaaga acaagccaag cgacgtgtac acctgggctt actttttgct gagtatgtaa
    14161 aaaaacacga aattgttgca gacaacgaca aagttaatgc tatgattgat aaatttgcca
    14221 gtgcttacga aagtcctgat gagcttcgcg cgtggtatca aagcagcaaa gaacatatgg
    14281 ctgaagttga agcattagtt atggaagata tggttgctga taaaatagca gaagatgcga
    14341 aacttaaata taaaaatatg gattatgatt cagtgatgaa tcccaaaaag ggcactgaga
    14401 aaaaaggaga gtaagcatgc caggctattc agataatatt atccgtaatg caagcggatt
    14461 aataccaatg gtaattgagc aaacctcgcg aggtgagcgt tcctatgata tttactcaag
    14521 attattaaaa gaaagaatta tttttctctt gggtgaagta gaagatcata tggctaacct
    14581 ggttgtggct cagttgttgt ttctggagtc tgaaaatcca gagaaagata tatcttatat
    14641 attaattctc ccggtggagt cgttactgca ggtcttgcta tttacgacac catgcaattt
    14701 attaagccag atgtaagtac attatgcata ggccaggcag caagtgcagc tgccttgcta
    14761 ctttgtgccg gtgctgaagg caaacgattc tgcttgccta actcaagagt tatgatacat
    14821 cagcccttgg gtggctatag aggccaagca acagacatag aaattcatgc tcgagagacc
    14881 ttagcagtta gggaacgttt gaataatatt atggctaaac acactaaaaa aacacctgat
    14941 caaatcatgc gtgatacaga aagagataac tttatgagcg ctactcaggc tatggagtat
    15001 ggtctaatcg ataaagttct ctatgacaga caagttgctg gacattctac agacttataa
    15061 ctttgctaga tactcctggt ttatctgagt ataatataag ttggtttgat agtaatgtgg
    15121 tggcgcaata ttatctgatt gtctaaaatt ttacatgtaa ttactattaa ttagtaatag
    15181 ctgtaagaag gggtttggtt atgagtaaat ccggtaacgg aaatggagat aaggtcttat
    15241 attgctcttt ttgtggcaag agtcaacatg aggtaaaaaa attaattgct ggaccttctg
    15301 tgtttgtttg tgatgaatgt gtagagctat gtaatgatat tattcgagaa gaaacacatg
    15361 aaactcatga agaaactgag gcacgtctac cgacaccaaa agaaatatct aactttctcg
    15421 atgaatacgt aatagggcaa caacatgcca aaaaggtttt atcagtagca gtatataatc
    15481 actataagag attacagcat aaaagcgagg atggtgttga actcggtaaa agtaatatac
    15541 tacttatagg acctactggc agtggtaaga ctcttttagc tcaaaccctg gcgcggattc
    15601 ttaatgttcc ttttgccatg gctgatgcca cgacccttac tgaagctgga tatgttggtg
    15661 aagacgttga aaatatcatc cagaagttat tacagaagtg tgactatgac gtagataagg
    15721 cgcagcaagg tattgtctat atcgacgaaa tcgataagat ttcacgcaaa tcagataatc
    15781 catctatcac cagagatgtt tccggagaag gtgttcaaca agctttactg aaattaattg
    15841 aaggtactgt tgcatctgtt ccaccgcaag gtgggcgaaa gcatccacag caggaatttt
    15901 tgcaagtaga tacatcaaat attttattta tctgtggtgg cgcttttgca ggtttggaca
    15961 aagtaatacg tgaacgcagc gataaatcga gcataggttt ttctgctcaa ttaaaaagca
    16021 aaaaaagcag taatgacgaa gtttcaaaag ttttaggtca attggaatca gatgatttaa
    16081 ttaaatatgg gttaattccc gagtttgttg gacgtttgcc tgttgtaacc actttacagg
    16141 aacttgatga agcggcattg attgatatat taacgagacc gaaaaatgct ttaacaaagc
    16201 aatttcaatc acttttcaaa atggaaggtt ctgaacttga gtttagagac gaagctttga
    16261 ttgctattgc taaaaaggcc ctcgaaagaa aaatgggcgc aagaggtttg cgatcaatac
    16321 ttgaaaatat tcttttagac accatgtatg acttaccttc cttggaaggt gtgaataagg
    16381 ttgttattga tgagagtgta gtgaatggac tatctaaacc gattctcatt tatgaacagg
    16441 atgagaaaaa aagtgcgtct ggtagtaaag attaagtaag atgttgattt ctgcaataat
    16501 ttaaattgtt gcagaaatca tt
//
LOCUS       DATNSN010000068        16390 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.068, whole genome shotgun sequence.
ACCESSION   DATNSN010000068 DATNSN010000000
VERSION     DATNSN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.068"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            145..2076
                     /locus_tag="VAH76_001730"
     CDS             145..2076
                     /locus_tag="VAH76_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386347.1"
                     /translation="MPHASDENQASKPTDLVFILAIAGRIDAQSRILAQKLDKSRSIY
                     YAYAVMDSLSSSYSMFKYFFDLFASPNDNDAMHEFLNSPTGLITIAVESVFLVTFSVL
                     ACQFENEKEDYYKKFIANAWPYFRDVMKGLKNAYKGWRSAVVAFSLIGGVDAKFLILP
                     MGVILGVFAAANRFWIRSMLEDRKTMMSENIALLMEIKKLISLSHEESFSYFKRIRSQ
                     TMQTRNFAFWAVAAGGLLDGLYLYVGVLSLAVLSPQLLMAMALICAFYTVACVITRIY
                     EEYDFQLRLLVTQTKCQLALITKELETNYAKLLLLQDKMDLDKADQEELKRLKRLVHN
                     LITRFDDSRKLLSQQSNRTYLSSALLGMKNGLYAYGALASVLFLVGSILVMAGVSFPP
                     ALLTACIILGLVLVLSFTIHSLVSNYWHQKKQETQKQRPYERLIEWNNCLNDEFNKTE
                     LLAPEEFNKLLNDGLSLDPSPQFFFQEWFEVLRSLFSGFGKGQKFIDFAGNSLQELGE
                     DGHYRDTPVMYVLSVFSALLFGVTLALRALAKGLGRTPLAQQAKLTEDSKEASVSPEI
                     TKGVMQKTEEQRESIQSEKTRTDSPGHTDSLSLFGIFETKTKTKRNQPDSPSIPHSKS
                     ESALNQLIPSQNSTILGLA"
     gene            complement(2042..2632)
                     /locus_tag="VAH76_001731"
     CDS             complement(2042..2632)
                     /locus_tag="VAH76_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HEO1386348.1"
                     /translation="MQESFDPSTITTLHKTSIIRWKANGIEYQHKQFLQLVEENHAFN
                     FQLWHAEDKARRNDMGFEFVYQAKREIDAFNQQRNNRMEAMDEWLYKALQPENHNHCP
                     VNSESPGMIIDRLSILSLKSYHMDLQTKRQDVGVEHRIACTQKLEIIQQQLNQLALCL
                     DELLNEVRAKTRTFRVYHQFKMYNDPKLNPELYCSD"
     gene            complement(2859..3587)
                     /locus_tag="VAH76_001732"
     CDS             complement(2859..3587)
                     /locus_tag="VAH76_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO1386349.1"
                     /translation="MKRFMFILLIFINFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFG
                     FNIDLMNSICKIIEEECQYKGMNIQQLYTNLNAGKVDIMLAPTPIVSTAGTNYIFSLP
                     YLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
                     MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMK
                     INKALLQIEKNGTYLEIYNTYFGD"
     gene            complement(3679..4125)
                     /locus_tag="VAH76_001733"
     CDS             complement(3679..4125)
                     /locus_tag="VAH76_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEO1386350.1"
                     /translation="MTIWVDADACPKMIKDILFRAAIRTNTNLILVANSYLTYPNSPF
                     IRSVLVEKGYDRADHYITSHMKAKDLVITADIPLAAEVIVKQGLAMSPRGELFTANNI
                     KQRLTLRDINEQLRSAGERTGGPSALSAKEKTNFANALDRWLVKSK"
     gene            4236..8210
                     /gene="lem1"
                     /locus_tag="VAH76_001734"
     CDS             4236..8210
                     /gene="lem1"
                     /locus_tag="VAH76_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem1"
                     /protein_id="HEO1386351.1"
                     /translation="MKQLISIIKGQIQQLPIPDLKHLLLSHLKEDAHDLTELFCQQTP
                     AQKNQFSHNQSVPFGLPASLSVLAIVLTYAPNLLHRELSEQLLTMLDSIRETETSVDN
                     QLAERCTEQLNYYSDAKPVLIALKERGAKDLIQILEQADERSLLAFSHLAKDTDLNVL
                     IETFLVKNNKDLDLVNRNHLVQFLRQPDLAGWLTRFLDSLIDGSINALSIRLLLSLIS
                     SVKAIEKLKAARACLAQYPFSIDLHAMRAVFDFYQYELKRDLFSPESHLVMQLLHDLL
                     KNTKLDKQQEFFQGLSQETVLLIIEYCIAEYNKTTGEEKQNCKNLLTRLCSAFSDSSQ
                     DFLGKIRTKINQLDPYLFDTPQLYQLASDLITKSCNSGKESLSSSPIALLLSMPQFVG
                     VCTPKVLKQLVERYSLYTLTLNQKDYKQDLCFKEYAPYFDEINEHMAQIERNLLIHPE
                     AQSFWLDKKYSLLQFRVEISKSALLHQMECLLEANKGGEELNNKGLEVLYHYYALSYP
                     NLRSDLFLKVVNHIYSRFFIKGNNETEARDGLFHWLKQNFPLIDKLPKDLDRKKEIFL
                     YNSAGKKIGFLGESNAAMTFVNEEIVLLAQTGSMDINEPLYDQEGLVLGYLTEESCLR
                     SESAIHKNAAAWVLAGLSKSDLESSSFGLESLLHNVLFENSIGVLYQCESICLDANKR
                     IWLNNKLLKEIQEAPGEIPASVLYPLLDHFEDETVFELLGVIRNKNNALELFHYILNR
                     DSRCSILLNGLYESDFQRFLEHQGADVCLADYLVNYYDKPWFSEVLLRFAYHGKKYKK
                     PDLLSSALTFITKEISRDKKQETICDAVLDRLISSEACAALVLKEFLGDRTHNSIQRV
                     NNPEIGKVSQFFDRRHIVSLIAQLNKTSYWEQNAQYKLALHILANQHGRLFSETDLNP
                     DSPQVWQGKELKELACFTSRHLSKKRPFDNKHDIGYRVLGELLFRCANSGITSLFYKQ
                     KTLNRAITRLSITEPFLVRMVDKLWMPKGIRELYGKSLFFKGSWFDDQVFLQKELKDH
                     HPLLDWSHFIKQTWNKRDKQKKSPIICTYLLSYTGRKSVLSRLLQDYFNSFQGMPDYI
                     RPVSQLLRFFPQRDVSAVIYDALEAEMIKKPQLLDRQILSDMAFYYGKQLARKDTSFP
                     QADINLLTYWGQNKHYSLVKRGCELLVRDCEDKGVKKRLVSAAKEAEVEQDLSSSVQT
                     FYFGLIKFFKRLWHYGFNAENSSSKIIKFCDDPTPGPTRKKAGDLINAPVAESRTNSL
                     YLGFSERRKQLISLLATIKSSPAHFTRVVNYSDNTQTFFGEQPKQSIDYNTPAIPSSF
                     ESIM"
     gene            8428..8892
                     /locus_tag="VAH76_001735"
     CDS             8428..8892
                     /locus_tag="VAH76_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386352.1"
                     /translation="MRILLSFFLLLLSTGLFAESTFPDGCRAVPVQGQSVTLKAKKNK
                     LVFIHNVTNLDLWITHPVTDPGASAGWTSRLQAGKWSALVVDKPAFILNCIESRPGHE
                     QQIPCENAIAVCQWKGVKIPASNQGTFWVAEDLSLAALNAAVGGRGFVLPEK"
     gene            9288..11468
                     /gene="rnr"
                     /locus_tag="VAH76_001736"
     CDS             9288..11468
                     /gene="rnr"
                     /locus_tag="VAH76_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO1386353.1"
                     /translation="MSKKSKDPFYKREREKYAEPIPSREYIMEILDEYGRPMSRSKLF
                     DKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFF
                     IPDDGSEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFF
                     TDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRSQAIGRVIHILG
                     EHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLRNLPFVTIDG
                     EDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVP
                     MLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAW
                     LEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRGAMDFETTETRIEFDENKKIKCIV
                     PVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELGL
                     SLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYT
                     HFTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREV
                     ITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAK
                     HRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE"
     gene            11461..12210
                     /gene="rlmB"
                     /locus_tag="VAH76_001737"
     CDS             11461..12210
                     /gene="rlmB"
                     /locus_tag="VAH76_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO1386354.1"
                     /translation="MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKA
                     ENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALPEYGESDLVTLLERSKKPVLILIL
                     DGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
                     RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSL
                     PMMGSVESLNVSVATGVSLYEVIRQRGQFRE"
     gene            12203..12853
                     /gene="rpiA"
                     /locus_tag="VAH76_001738"
     CDS             12203..12853
                     /gene="rpiA"
                     /locus_tag="VAH76_001738"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO1386355.1"
                     /translation="MSELKIKAAKAAIAYIEDDMVIGVGTGSTVNFFIKELAAIKHKI
                     EACVASSKATEALLRAEGIPVIDLNSVQDLPIYVDGADEVNERGEMIKGGGGALTREK
                     IVANVATQFICIVDESKVVKRLGEFPVAVEVIPMARSFVARQIVKLGGDPEYREGFVT
                     DNGNIILDVFNLNFSTPMALEDSLNVIPGVVENGVFAKRLADKVLVASASGVNNLK"
     gene            12917..14296
                     /locus_tag="VAH76_001739"
     CDS             12917..14296
                     /locus_tag="VAH76_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5' nucleotidase"
                     /protein_id="HEO1386356.1"
                     /translation="MDTHKVFVNRIINMRKIKLIGLDMDHTLIRYNSKNFESLVYDLV
                     KERLAESFHYPEEIKKFKFNFDDAIRGLVIDSKNGNILKLSRYGAIRLSYHGTKQISF
                     SDQKKIYRSIYVDLGDPNYMAIDTSFSIAFCILYGQLVDLKDTNPDKMPSYQAIAQDV
                     QYCVDKVHSDGTLKNIIIKNLKKYVIREKEVVEGLKHFIHYGKKIFILTNSEYSYSKL
                     LLDYALSPFLDKGEHWQDLFEFVITLANKPRFFYDNLRFLSVNPENGTMTNVHGPIVP
                     GVYQGGNAKKFTEDLGVGGDEILYIGDHIYGDILRLKKDCNWRTALVVEELGEEIASQ
                     IRALPIEKKIVEAMSIKKELEQKYVDLCTRSIDESSQQYDQEIHDLQLQISTVDLQIS
                     RLLQEQNSFYNPKWERVFRAGAEESYFAYQVDRFACIYMEKLSDLLEHSPMTYFRANR
                     RLLAHDIDI"
     gene            14680..15873
                     /gene="ceg4"
                     /locus_tag="VAH76_001740"
     CDS             14680..15873
                     /gene="ceg4"
                     /locus_tag="VAH76_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector phosphotyrosine
                     phosphatase Ceg4"
                     /protein_id="HEO1386357.1"
                     /translation="MPKVIIFTDFDGTVTGRSGNETVFTDFYQSLLQGYKKDVEQDYK
                     NTPMKDPIEAQALFEAKYGKYNENFDHDQQDVDFLMSPEAVAFFHEVLKNDDVTVNIV
                     TKNRAEYIKAVFKYQGFSDEEIEKLTILESGYKFNDVNSRLNHPTERANRVYILDDSP
                     TDYAEMLRAVKGKGYNEEEIRGHRKNPGEFEWSQYLEDVREMFPPKENSPEEVRLPYE
                     QGDEVSIGEEIPVLLQEDNLSRREETATVLSNQPVPSSDFRTLKIMGAFAGIGFGFGF
                     ALGVTLVATGVFAPPGVGLLGALTLGAISGTGAAALSSAIGFAVAKGTEITPEPVSEE
                     PVPAVKQSSHEAMRGLGGSQLLSTNNIHPVGHFPSVLSGRTPTKGKEEQCQKEYVLTN
                     PLNNS"
     gene            16035..16376
                     /locus_tag="VAH76_001741"
     CDS             16035..16376
                     /locus_tag="VAH76_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO1386358.1"
                     /translation="MNQLPNCPKCLSEYCYEDGDVLICPECTHEWPRNSNNNDEESAK
                     IIKDANGNLLQNGDTITVVKDLKIKGSSQVIKVGTKVKNIRLVDGDHDIDCRIDGIGA
                     MKLKSQFVKKI"
BASE COUNT         4957 a         2809 c         3543 g         5081 t
ORIGIN      
        1 taattttgct aataggatgt gttattatga agggtttgag gttattgttg tattttttta
       61 acccaggatg aaagtttaat tgacatcaag tctaattatg catacaatag cgcccaatct
      121 tagctgatag ccccatacct atacatgcct catgcgagtg atgaaaatca ggcttccaag
      181 cctactgatt tagtcttcat tttagcaatt gctggtcgta tcgacgcgca aagtagaatt
      241 ctcgctcaaa aactcgataa aagtcgcagt atctattatg cctacgctgt tatggattct
      301 ctaagctcct cgtacagcat gttcaaatat ttttttgatc tgtttgcatc tcctaatgat
      361 aacgatgcga tgcatgaatt cttaaattct ccaaccggtc ttattaccat tgcagttgag
      421 tcagtatttt tagttacctt ttctgttttg gcctgtcaat ttgagaacga aaaagaagat
      481 tactataaaa aatttattgc taatgcctgg ccatattttc gtgatgtcat gaagggcctg
      541 aaaaatgctt acaaaggctg gagaagcgct gttgttgcat tcagtttaat tggaggagtg
      601 gatgcaaaat ttcttattct tccaatggga gtgattttgg gggtatttgc tgcagccaac
      661 aggttttgga tacgtagcat gctggaagat cgtaaaacaa tgatgagtga gaatatagcg
      721 cttctcatgg aaataaaaaa attaatttct ttatctcatg aggaaagctt ttcttatttc
      781 aagcgaatca gatcccagac catgcaaact cgtaatttcg cattctgggc tgttgctgca
      841 ggcggactac ttgatggttt atatctctac gttggtgtat tgagtctggc tgtattgtca
      901 ccgcagttgc ttatggcaat ggcgcttatt tgtgcctttt atacagtggc ttgtgttatt
      961 acccgtattt atgaggaata tgatttccag ttgagattat tagtgacgca aaccaaatgt
     1021 caattggctt taattaccaa agaactggaa actaattacg ccaaattact cttattgcaa
     1081 gacaaaatgg atctggacaa agctgatcag gaagagctca aacgactaaa aaggttagtc
     1141 cataatttga taacccggtt tgacgatagt cgtaagttat tatcccaaca atcgaaccgc
     1201 acttatttat catctgcttt gttgggtatg aaaaatggat tatatgctta tggcgcgctt
     1261 gcaagtgttt tgtttttagt gggttctatt ttagtaatgg caggtgtttc ttttcctcct
     1321 gccttgctta cagcttgtat tattttaggt ctggtattag tgctcagttt cactattcat
     1381 tctttggtga gtaattattg gcatcagaag aagcaggaaa ctcaaaaaca acgtccatat
     1441 gagcgattga tcgagtggaa taattgtctt aatgatgaat ttaacaagac tgagctgttg
     1501 gctcctgaag aatttaataa attactaaat gatggattgt ctttagaccc ttcaccacaa
     1561 tttttctttc aagaatggtt tgaagttttg cgttccttgt tttccggatt cggtaagggg
     1621 caaaaattta ttgactttgc ggggaattcg ttacaggaac tgggcgagga tggccattat
     1681 cgtgatacgc ctgttatgta cgtcttatct gtcttcagtg ccttattgtt tggggttact
     1741 ctggctttaa gagctctagc caaaggattg gggcgaactc ccttggcgca gcaggccaag
     1801 ctgacggagg acagcaaaga agcgtcggtt agccctgaga taacgaaagg agttatgcag
     1861 aaaaccgaag aacaaaggga atcaatccaa tctgaaaaaa ctcggactga ttccccaggg
     1921 catacggatt ctctgtcttt attcggtata tttgaaacca aaaccaaaac caaaagaaat
     1981 caacccgact ctccatcaat tcctcattcg aaaagcgagt cagcattaaa tcaattaata
     2041 cctagtcaga acagtacaat tctgggttta gcttaggatc gttatacatc ttaaattgat
     2101 ggtaaactct gaacgtacgt gttttagctc gtacttcatt aagtaattca tcaagacaca
     2161 aagccaattg atttaattgt tgctgtatga tttccagttt ttgtgtgcaa gctattctat
     2221 gttctacgcc tacatcctgg cgtttggttt gtaaatccat atgatatgac ttcagtgata
     2281 aaatagataa tctgtcaata atcatcccag gtgattcgga attgacaggg caatgattat
     2341 ggttttcagg ctgtaatgct ttatacagcc actcatccat tgcttccatg cggttgtttc
     2401 tttgctgatt gaaagcgtct atttctcttt tggcctggta aacaaattca aagcccatat
     2461 catttcggcg cgccttgtct tctgcatgcc ataattggaa attaaaagca tgattttctt
     2521 ctacaagttg aaggaattgt ttatgttgat actctatgcc attggctttc caacgtatga
     2581 tgctcgtttt atgaagggta gtgatagttg atggatcaaa actttcttgc atgtcatatc
     2641 ctgcagatta atttggattg tactttaaga tgagtttagt ctatgaaaat atacctattt
     2701 ccataacttg aactcgactg ttttgaacta tatctcatgc attgaggctc aatacattat
     2761 gagagacagg ttcagttggt attagtttaa cctgaattct ggagttggat ctagtttcgg
     2821 gtttttgaaa tgcctggtga attgctcagg ataatatcct aatcaccgaa ataggtatta
     2881 tatatttcca aataggttcc gtttttttct atttgtaata aagccttgtt gattttcatg
     2941 atgagcggag cattattctt gagggctagt attccatagc cattacctat aggaatggga
     3001 tctcccacaa atttgagctc agtcgcggaa ttgctcatcg cgtatttcgc tgcattggca
     3061 ttaagcacta ctgcttcgat ttctcgattt gacaatgcga ttgccatatc gggaagtttg
     3121 gaaaattgca tcaaatcaac ataatcctga aaatgctcct ggaggaggcc accgtataaa
     3181 gtgtatttta aagtaccaac ttttttacct tttagatccg ttaaggcatt gatatggtca
     3241 ttagctttca gggttacaat ttgggcattg ctcggcaaat agggaagact aaagatataa
     3301 ttggtgccag cagtggatac gattggagta ggtgccagca taatatcaac ctttcctgcg
     3361 ttgagattgg tatatagctg ttgaatgttc atgcccttgt attggcattc ttcctcaata
     3421 atcttgcaaa tcgagttcat caaatcgatg ttgaatccga aataaaactc acctccacca
     3481 gatcttgata caaatggtgg agcaaaatct ggaacgccaa taatgagtgg ttttccaaag
     3541 gcgtggaaat ttataaagat caataagatg aacatgaatc gtttcatgtt tttaagtcca
     3601 tttaggatgt acttaaaatt taggaggagc aatcgatttt gtcaaattaa agtcaatgtg
     3661 gttttttaat tgatgctttt atttgctttt tacaagccaa cgatccaggg cattggcaaa
     3721 attggttttt tctttcgcgc ttaatgcaga ggggcctcct gtgcgctctc cagctgaacg
     3781 caactgttca ttgatatctc taagagtcag acgctgtttg atattatttg cggtaaacag
     3841 ttctcctctt gggctcattg ctaacccttg cttaactatt acttctgctg ccaaaggaat
     3901 atccgctgtg attaccaaat ctttagcttt catatgactc gtaatataat gatctgctcg
     3961 gtcatagcct ttttctacaa gaactgagcg aataaacggt gaatttggat aagtaaggta
     4021 ggaattagct accaggataa ggttggtatt ggttcgtatg gcagcgcgaa agaggatatc
     4081 ttttatcatt ttcgggcaag catcggcatc aacccagatc gtcatttggt atcctaaagt
     4141 tttcaaatca atagtttaag ggtatagtaa aaacattatc cttataaatc cctattgggg
     4201 aaaggattat tttaggatgt tagggaagtg tcgtaatgaa gcagttaata agtataataa
     4261 aaggacaaat tcaacagctc cccattcccg acctgaagca tttgttgtta agtcacctta
     4321 aagaagatgc tcacgattta acagaactct tttgccaaca aaccccggct cagaaaaatc
     4381 aattctcgca taatcaatca gtcccatttg gtttaccggc ttcgttgtct gtactcgcaa
     4441 tcgttttgac gtatgccccc aatctgctac accgagagtt atcagagcag ttgctgacaa
     4501 tgttagattc aataagagag accgaaacca gtgttgataa tcaattagca gagagatgca
     4561 cggagcaatt gaattattat agtgatgcaa aacctgtcct tatcgcgctg aaagaaagag
     4621 gagccaagga tttaattcag atcttggagc aagccgatga gcgcagtttg ttagcctttt
     4681 ctcatctggc taaagatact gatttaaatg ttttaattga aaccttttta gttaaaaaca
     4741 ataaggattt ggatttagtc aatagaaacc atttggtaca atttttacgt caacctgatt
     4801 tggcaggatg gttaacccgt ttccttgatt cattaatcga tggatctatc aatgctctct
     4861 caattcgatt gttgttgtct ttgatttctt ctgtgaaagc gattgagaaa ctgaaggcgg
     4921 ctagagcctg ccttgctcag tatccttttt caatagattt gcacgccatg cgggctgtgt
     4981 ttgactttta tcagtatgaa ttaaaaagag atttattttc tcctgaaagc catttagtca
     5041 tgcagttgct gcatgattta ttaaaaaaca ctaaattaga caaacaacaa gagttttttc
     5101 agggcttaag tcaggaaact gtgcttttga tcattgagta ttgtatagcg gaatataaca
     5161 aaacgactgg ggaagagaag cagaactgta aaaacttgct taccaggtta tgttcggctt
     5221 tttctgattc cagccaggac tttttgggga aaataagaac aaaaattaat cagctggatc
     5281 cttatctctt tgatacccct cagctatatc agctggcatc cgatttaata actaaatcat
     5341 gtaactcagg aaaagagtcg ttatccagtt ctccaatcgc tttattgtta tccatgccac
     5401 aatttgtcgg ggtttgtacg cctaaggttt taaaacaatt agttgaaaga tacagcttgt
     5461 ataccttaac tctgaatcag aaggactata agcaagacct ttgttttaaa gaatatgctc
     5521 catattttga tgaaatcaat gaacatatgg ctcagattga gcgaaatctg cttattcatc
     5581 cggaagctca gtcattttgg ctggataaaa aatacagctt attgcagttt agagttgaga
     5641 tcagtaaaag tgctttgctt caccaaatgg agtgtttact ggaggcgaac aagggaggag
     5701 aggagctaaa taataaggga ttagaggtgt tatatcatta ttacgccttg agttatccca
     5761 atttacgttc ggatttattc cttaaagtgg taaatcatat ttattcacgg ttttttatta
     5821 aggggaataa tgaaaccgaa gctcgggacg ggttatttca ctggttaaaa caaaattttc
     5881 ctctaattga taagttgccc aaagacttag acagaaaaaa agaaattttt ttatataact
     5941 ctgccgggaa gaagattgga tttcttggtg agtctaatgc tgcaatgact tttgttaatg
     6001 aggaaattgt attattagct caaacgggct ctatggatat caatgagcct ttatatgatc
     6061 aagagggttt ggttctcggg tatttaacgg aagagagttg cctaagaagc gaaagtgcca
     6121 ttcacaaaaa tgctgctgcc tgggttttag ctggtttgtc caaaagtgat ttggaaagtt
     6181 cctcgtttgg attggagtcc ctgttacaca atgtgttatt cgagaactcg ataggggttt
     6241 tatatcaatg tgaatccatt tgccttgatg ccaataagcg tatatggctg aataataaat
     6301 tattaaaaga aatccaagag gccccaggag aaatccctgc gtctgtttta tatccattgc
     6361 ttgatcattt tgaggatgaa acagtatttg aattattagg cgtcatcagg aataaaaaca
     6421 atgcgcttga gctttttcat tatattttaa accgagactc aaggtgttct attttactga
     6481 atggcttgta tgaatctgat tttcaaagat ttttggaaca tcaaggcgcc gatgtttgtt
     6541 tagctgatta tttggttaat tactatgata aaccctggtt ttcagaagtc ttactacgat
     6601 ttgcctacca tggtaaaaaa tataaaaagc cagatcttct aagtagtgcc ttgactttca
     6661 ttaccaagga aatttctcga gataagaagc aagagacaat ctgtgatgct gtactggatc
     6721 gtttaataag ttctgaagca tgtgccgcat tagtgttaaa ggagtttctt ggtgatagaa
     6781 ctcataattc cattcagagg gtaaataatc cggaaatcgg caaagtaagc caattttttg
     6841 ataggcggca tatagtgtcg cttattgcac agttaaacaa gacttcttat tgggagcaaa
     6901 atgcgcagta taaattggct ttacatattt tggctaatca acatggacgc cttttttctg
     6961 aaactgattt gaatcccgat tctcctcagg tctggcaagg taaagaatta aaagaactgg
     7021 cttgttttac aagcaggcat ttatccaaaa aacgtccttt cgataataaa catgatattg
     7081 ggtatcgtgt tttaggggag cttcttttca gatgcgccaa ttcgggaata acgtcattat
     7141 tttataagca aaaaacgttg aatcgcgcaa taacccgatt gtcaatcaca gaaccttttt
     7201 tagtcaggat ggtggataaa ttatggatgc ctaaaggaat cagggaactt tatggaaagt
     7261 ctttgttttt taagggttcc tggtttgatg atcaagtctt cttgcaaaag gaattaaaag
     7321 accatcatcc tctgctggac tggagtcatt tcattaagca aacctggaat aaaagggata
     7381 aacagaagaa gtcccccata atatgtactt atttgttaag ttatacgggt aggaaaagcg
     7441 tattgtcgcg cttattacag gattatttta attcttttca aggaatgcca gattacatca
     7501 ggccagtcag ccaattattg agatttttcc ctcaacgcga tgtgtccgca gtcatatacg
     7561 atgcgctgga agctgaaatg ataaaaaaac ctcagttatt agacaggcaa attttaagtg
     7621 acatggcctt ttattatgga aaacagttgg ccaggaagga cacaagtttc ccccaggctg
     7681 atattaattt attaacgtat tgggggcaga ataaacatta ttctttagta aaaagaggat
     7741 gtgaactatt agtcagggat tgtgaggata aaggagtaaa aaaacgattg gttagtgctg
     7801 ccaaagaagc ggaagtagaa caggacttga gttccagtgt gcaaacattt tattttgggc
     7861 taattaaatt ttttaaaaga ttatggcatt atggttttaa tgctgaaaac agctcatcaa
     7921 aaattataaa gttttgtgat gatcctactc ccggccctac tcgtaaaaaa gcaggggatc
     7981 tcatcaatgc accggttgca gaaagcagga ccaattcttt gtatttaggc ttttctgaaa
     8041 gacgcaagca gttaattagt cttttagcta ctattaaaag tagtccggcg cacttcacac
     8101 gagttgttaa ttattcagac aatactcaaa cattctttgg agaacaaccc aaacagtcaa
     8161 ttgactataa tactcctgca ataccaagta gctttgaaag tataatgtag aattgccctt
     8221 atagggcatt tataccttcg ctcatatttt tttattagcc aggcgttctg catgcattaa
     8281 tcaacccgat gcatgactta tcaatggaag tcttatatga gtagggatat tatatgagtg
     8341 ttttcttaaa tcgatgccga tattgatgat ggatttatta gtgaagattt aataattttt
     8401 ggttacataa acactatggg gtgaatcatg agaattttgc tctcgttctt tcttttattg
     8461 ctttcaacag ggctttttgc agaatctact tttcctgatg gttgtcgggc tgttcctgta
     8521 caaggtcaat cagtgacttt aaaagccaag aaaaataagt tggtgtttat tcacaatgtg
     8581 accaacttgg atctatggat tactcatcca gttaccgatc ctggggccag cgctggttgg
     8641 acaagccgat tgcaagctgg taaatggtca gctttagtag tggacaaacc tgcctttatt
     8701 ttaaattgca tagaatcaag gccaggacat gagcaacaga taccttgtga aaacgctatt
     8761 gccgtatgtc aatggaaagg agtaaaaata ccggcaagca atcaaggtac tttctgggta
     8821 gcggaagacc tgtcattagc agcattaaat gccgcagtcg gtggaagagg ctttgtattg
     8881 ccggagaaat aatttatcaa tgaatgtctt ggggcatgac tttggattct atgctgcaac
     8941 atgtcatatc aattttcatt acaaatcagg atagccgctg atgaggctat cctgagcctt
     9001 tggcaaatag tttacccagc tgccatgctg cccactcttg agtccttatg aaaacttaaa
     9061 actttaataa tggggtaaat ggattgagat tatgcaatat aaataagcct gattttatca
     9121 tgagggcttc ttgtcttgta agattggaaa aaagatgaat aaaaggcgtt ttatacagaa
     9181 ttctctgata gttggcagca tattcttgct agttatagtg ataaatggta gaacaggact
     9241 taagaggttt tagttagcat taagtttgaa gagaaaaatg gtgaaaagtg agtaaaaaat
     9301 ccaaagatcc cttttataag agggagcgcg aaaaatacgc cgagccaatt ccaagccggg
     9361 aatacatcat ggaaatcctg gatgaatacg gtaggccaat gtctcgcagt aaactgtttg
     9421 ataaattgga tttgagctct gagagccagc aggaatcctt gggttttcgc ttgaaggcca
     9481 tgctgcgtga tggccaaatc atgcaggaca ggcgtggacg cttttgttta ttgcaaaaaa
     9541 ttaatttatt gcgtggaacg gttcaaggcc atcctgatgg ttttggattt ttcatcccgg
     9601 atgatggttc tgaggatatg gtgctctctg ctaaagaaat gagggctgtc atgcatggag
     9661 atatggtgct tgcctatcaa tctggattgg atagaagagg aaggccagaa gctaaaatcc
     9721 acgaagtcct ggaacatgcg aataccacgg ttgtaggtcg attttttact gatcatggcg
     9781 tgaattttgt cattcctgat agcaaacgtc tgactcagga tatttcaatt ccccaagaat
     9841 tcattggcga ggccaaaaat ggccaaatag tactggttga gttaatcgca taccctaaca
     9901 agcgttcgca agctataggc agagtaatcc atattttggg tgagcatatg gctcctggca
     9961 tggaaattca ggtggcaatc catgcccacg gcattccttt cgaatggcca gatgatgttc
    10021 ttgcggaagt gggtaaaatt ccacaacaga tttccgagga acaattaaag ggtcgaaccg
    10081 atttgcgtaa tcttcctttt gtaaccattg acggggaaga tgcaaaagat tttgatgatg
    10141 ctgtgtattg ttacaaaaag cccaaaggcg gcttccaatt gtatgtcgct atcgcagacg
    10201 tcagtcatta tgtggcgaag gattctgctt tggatcaaga agcagcaagg cgaggaaatt
    10261 cagtttattt tcccggtaaa gtggtgccca tgctaccaga ggccttatcc aatggcgttt
    10321 gctcattaaa tcctcatgtt gaccgcttat gtatggtggc tgagttatcg attacccaag
    10381 aaggaaaaat atcccgatcc cgcttttatc gggcagtgat tcattctcat gctcgtttga
    10441 cctacacgtc agttggggca tggttggaac aaggcaaggc agatgaaaaa catcaagcct
    10501 tgtggcctat gcttgagtct ctttatgatt tatatcatgt tctgctcgat acccgtagac
    10561 ttagaggtgc tatggatttc gagactacgg aaactcgtat cgaatttgat gaaaataaga
    10621 aaataaaatg tatcgtgcct gtaattcgta atgacgctca taaattaatt gaagaatgca
    10681 tgctggcagc gaatgttgcg acagcaagat ttttagaaaa ggctgaaatt cctactttat
    10741 accgagttca tgcggctcct gaagaggata aaattactgc attgaggcag tttttagggg
    10801 agctgggatt gtcattggga ggggggagaa aacctggacc caaagatttt cagcgcacca
    10861 tgaacatgat tggtgaccga ccagagaagc atcttattga aacggtaatg ctgcgttctt
    10921 taaaacaggc tcagtacgtt gaatcgaatg aaggtcattt tggtttggcc tattccgctt
    10981 acacgcattt tacatcacca atcagaagat ttcctgatct attaattcac agagcaatag
    11041 ggcatttact ggataatcat cctgtttatg agtttgaata tagccatgaa gatatgaatc
    11101 gtttgggtaa acattcctcg atgacggagc gccgtgctga tgaagcgacc cgggaagtga
    11161 ttacctggtt aaagtgcgag tatatgcaag acaaattggg gcaggtattt aagggtcgaa
    11221 tatccgctgt gaccagcttt ggtatttttg tggagcttga tgaaatttat gtagaaggct
    11281 tggttcatgt cacttcttta aaaaacgatt attatacgtt tgactctgca aaacatcgtt
    11341 taataggggc tcgtggcggt caggtttatc gtttgggaga taaaatgact gtgttggtag
    11401 ctcgtgttga tttaaacgaa cgtaaaatag attttgagcc tgtcgaggat gaagtttgta
    11461 atgaatgagc agtatgttta tgggttgcat gcagtttctg cattattagc aaacccgcat
    11521 cgaatgatca aaaaactctt tgttaatcaa gatagaatag ataaaagact tcatgagttt
    11581 ttggtcaagg cagaaaacgc ccatgtccct atcgaaaagc taagcgcaca aaaaatgaat
    11641 cagcagtttt cagagtttac gcatcagggc gttgttgcac tcgcttcggc attacccgaa
    11701 tacggtgaaa gtgatttggt tactttatta gaaagaagta aaaagcctgt gttgattctt
    11761 attctggatg gtattacaga tccccataat ttaggcgctt gtttgcggtc ggctgatgca
    11821 accggtgttg attttgttgt gattcctaaa gataaaagtg cgaatattac tcccgtggtc
    11881 agtaaagtgg cttgcggggc tgctgaatca attcctttgg tgagagtaac taatttagca
    11941 agagctatgg acatcttaaa acaacagggt gtctggatct ttggagcagc tggcgaagca
    12001 gagcatttac tttatgcaat ggattgttct ggaaacatcg ctcttgtaat gggagcagaa
    12061 ggaaccgggt taagaagatt gactcgcgag cattgcgatg gattgttttc tttgcctatg
    12121 atgggaagtg tagagagttt aaatgtgtca gtggcgactg gtgtttcatt gtatgaagta
    12181 attaggcaaa gagggcaatt tcgtgagtga attaaaaatc aaagcagcca aagcagctat
    12241 agcttatatt gaagatgata tggttatagg tgttggaaca ggctctacag ttaatttttt
    12301 tataaaagaa ctggcagcaa tcaaacataa gatagaggct tgtgtagcca gttcaaaagc
    12361 aaccgaggca ttgcttcggg cagaaggtat cccagtaatt gatttaaatt cagtacagga
    12421 tttacctatc tatgttgatg gcgctgatga agtcaatgaa cgcggtgaaa tgataaaggg
    12481 ggggggagga gctctgactc gagagaaaat tgtagcgaat gtagccacac aatttatttg
    12541 tattgtcgat gaatccaagg ttgtgaaacg tttaggggaa tttcccgttg ctgtggaagt
    12601 cattccaatg gcccgtagtt ttgttgcacg acaaattgtt aaattagggg gagaccctga
    12661 atacagagaa ggtttcgtta ctgataatgg taatattatt ctggatgtgt ttaatctgaa
    12721 tttctcaaca cctatggcct tagaagacag tcttaatgta atcccgggtg tcgttgaaaa
    12781 tggagtgttt gccaaacgct tggcagataa agtgttagtg gcttcagcaa gcggtgtgaa
    12841 taatttaaaa tagtcgactt gtatacgttc atatctcaaa acattaattg ccaataattt
    12901 tataggttcc tcaaaaatgg atacgcacaa ggtttttgta aatcgaatta taaatatgag
    12961 aaaaattaaa ctgattggtt tggacatgga ccataccctc atccgatata attcgaaaaa
    13021 ttttgaatcc ttagtttacg atctcgtcaa ggaaaggctg gcagaaagct ttcactatcc
    13081 tgaggaaatt aaaaaattca aatttaattt tgatgatgcc attcgtggct tggtgattga
    13141 cagtaagaat ggcaacattt taaaactgag ccggtatggt gccattagac tgagttacca
    13201 tggcacgaaa cagatcagtt ttagcgatca gaaaaaaatt tacaggagta tttatgttga
    13261 cctgggtgat ccgaattaca tggcaattga tacttctttt tccatagcat tttgcatcct
    13321 gtatggccag ctggttgact taaaagatac caatcccgat aaaatgccaa gctatcaagc
    13381 catagcgcaa gacgtccaat actgcgtgga taaagttcac tcggatggta ccctgaaaaa
    13441 cataattatt aaaaatttga aaaaatatgt gataagagaa aaggaagttg ttgaagggtt
    13501 gaagcatttt attcattatg ggaaaaagat atttattctg accaattcag aatattctta
    13561 ttctaaattg ctgttggatt atgccttgtc cccatttcta gataaagggg agcactggca
    13621 agatttattt gagtttgtta ttaccttagc aaataaaccc cgtttctttt atgataactt
    13681 acgatttcta tcagttaatc ccgaaaacgg gacgatgact aatgtccatg gcccaatagt
    13741 tcctggagta tatcaaggag gtaatgccaa aaaatttact gaagatctcg gggtcggcgg
    13801 agatgaaatt ctctatatag gggatcatat ttacggtgat atattacgat tgaaaaaaga
    13861 ttgtaattgg cgtactgctt tggttgttga agaattaggt gaagaaattg catcccaaat
    13921 aagggctttg ccaattgaaa agaaaattgt tgaagccatg tccattaaaa aagagttaga
    13981 gcagaaatac gtcgatcttt gtacaagaag tattgacgaa agctctcaac agtatgatca
    14041 ggaaattcat gatttgcaat tgcaaatatc cactgttgac ttacaaattt caagattgtt
    14101 acaagaacaa aacagtttct ataatcccaa gtgggaaaga gtttttcgcg ctggagcgga
    14161 agagagctac ttcgcctacc aagtcgatag attcgcctgt atctacatgg aaaaattatc
    14221 cgatttactc gagcattctc caatgaccta ttttcgtgcc aacaggcgat tgttagctca
    14281 cgatatagat atttgatttt ttctattaat caggtaccgg gatcggacga gggggagtgc
    14341 tattcatccc ctacaaagtt attctatggc gtggtatgtg caagggaaca cgatctttat
    14401 cacgcaaaat actggatatg ctataatgaa gtgtgccaac cagactggtt ggtgcttttt
    14461 cataggcctt catgctgtct aaataatcaa ttgcaactat aaattataag ctttccatgc
    14521 agaaaacatt ttgataaaac tcataagcaa agtctattgc aatttgtacg gggaagatag
    14581 cctgataatt ttgtaatttt ctatacttaa tattctctta atttaaactt tgtacaatat
    14641 cgaatcattt aaaattaaga gaaatagtaa ggaaatacaa tgcctaaagt aattattttt
    14701 accgattttg atggcactgt aaccggaaga agcggtaatg aaactgtatt taccgacttt
    14761 tatcaatcac tgttacaagg ctataaaaag gatgtagagc aggattacaa aaacactcca
    14821 atgaaagatc caattgaagc ccaggcttta ttcgaagcaa aatatggaaa atacaacgaa
    14881 aattttgatc atgaccagca ggatgttgat tttctgatga gtcctgaagc agtagcgttt
    14941 ttccatgaag tactaaaaaa tgatgatgtg accgtcaata ttgtcaccaa aaaccgagcg
    15001 gaatacatta aagcagtttt taaatatcaa ggattttccg atgaagaaat cgagaaatta
    15061 accattctgg agtcgggata taaatttaat gatgtcaatt cccggcttaa tcacccaaca
    15121 gaaagggcca accgggttta tatccttgat gatagcccga cagactatgc tgagatgctg
    15181 cgtgcagtaa aaggtaaagg atacaatgag gaagaaattc gtgggcatcg taaaaatcct
    15241 ggtgagtttg aatggtccca atatttagaa gatgttaggg aaatgtttcc gcctaaagag
    15301 aactctcctg aagaggttcg attaccatat gagcaagggg atgaggtttc tatcggtgaa
    15361 gagatccctg ttttacttca agaggataat ttatcccggc gtgaggaaac ggctactgtc
    15421 ttatctaatc aaccggttcc gtcttctgat tttcgtaccc taaagattat gggtgctttt
    15481 gcaggcattg gctttggttt cggctttgct ttaggtgtta cattggttgc taccggagtt
    15541 tttgctcctc caggtgttgg tcttttagga gctttgactt taggtgctat aagtggtact
    15601 ggcgctgctg cactatcaag tgctattgga tttgcagttg ctaaaggaac agaaattaca
    15661 ccagagcccg taagcgaaga gcctgttcct gctgttaagc aaagcagcca tgaggctatg
    15721 cggggtttgg gtggctctca actactgagc acaaacaata ttcatccggt tggtcatttc
    15781 ccaagtgttc tatcaggaag aactcccacg aaaggtaaag aagagcaatg ccaaaaagaa
    15841 tacgtattga ctaatccact caataattca tagcatttct atttcgttgg ataaataatg
    15901 attaaaaaaa tcattattta tccggccatt ccagaatcgt tcaattacag atcaatgtag
    15961 tcagaaattt ttaaaatgct ttctattaga gtaaaatagt ttgtaatcaa tttaattcag
    16021 ggtttttcaa caaaatgaac caattgccca attgtcctaa atgcctttct gaatactgtt
    16081 atgaggacgg tgacgtatta atttgccctg agtgtacgca tgaatggcca agaaacagta
    16141 ataacaatga cgaagagtct gcaaaaatta tcaaagatgc caatggaaac ctgttgcaaa
    16201 atggagatac gataacggtc gtcaaggatt taaaaataaa gggctcttct caagtgataa
    16261 aggtaggaac taaagtaaaa aatatccgtc tggttgacgg cgatcatgac attgactgca
    16321 gaatcgacgg gataggcgcc atgaaattaa aatcacaatt tgtaaaaaaa atttaatagg
    16381 gaacttcgtt
//
LOCUS       DATNSN010000069        16330 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.069, whole genome shotgun sequence.
ACCESSION   DATNSN010000069 DATNSN010000000
VERSION     DATNSN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16330
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.069"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..115
                     /locus_tag="VAH76_001742"
     CDS             <1..115
                     /locus_tag="VAH76_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO1386359.1"
                     /translation="LIIIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG"
     gene            359..904
                     /locus_tag="VAH76_001743"
     CDS             359..904
                     /locus_tag="VAH76_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEO1386360.1"
                     /translation="MKLLFDFFPIVLFFIVYKFFGIYTATAVAMVASLTQVAFYRLKF
                     QHYEKMHLFSLAIIMVLGGATLFFQNPWFIKWKPTGIYWLSALVFYGSGYIGSKPLIQ
                     KMMEANINLTTKIWYRLNLAWTLFFIVMGALNLYVAYHYDTDVWVNFKLFGGVGFTLL
                     FVLIQAFYLTKHTDEKSFEKQ"
     gene            965..1642
                     /locus_tag="VAH76_001744"
     CDS             965..1642
                     /locus_tag="VAH76_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386361.1"
                     /translation="MRLLLVEDDELLGDAVKAGLTQFGYVVDWLKDGEAARAALKSES
                     FELIILDLGLPKLSGLSLLQNIRHDGNSTPVIILTARESIEDRVKGLDSGADDYLIKP
                     FDLNELSARIRALVRRSQGRADSVLQYKNITLDPAAHSVFVDDVLVNVPRREFALLQK
                     LLENSGQVLSREQLMQSIYGWEEDVDSNALEVHIHNLRKKLNANFIRTIRGVGYMAEK
                     NDSVLLS"
     gene            1639..3054
                     /locus_tag="VAH76_001745"
     CDS             1639..3054
                     /locus_tag="VAH76_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEO1386362.1"
                     /translation="MKSSIRKFLLINLLLAITITTTLTAIGNYYLDQKDIQDHLDTLM
                     AISALSYQALLGDDLHQRPLAKIQNALESIPQKINTYYQKRYLNDEPQENYLDKFNFQ
                     VWTNGGKLLLHSPTAPKIPLTAETDGFSDKRISNQDWRVFTTYNDKAGIRTVLAERYD
                     TRNELGHRIAQDDLYIMLLTFPLSGLLIWIIIGRGLDSLDRVAEEVANRAPSHLEPVD
                     LQEVPEEIKPVIDELNKLFFRLKEGFEREKRFAADAAHELRTPLAALKAQAQVALNSN
                     DIEEKNQALQKLIASVNRSTHIVQQLLTMSKLVPEATELHEQDEVNLSKLTREILAML
                     APSAVEKQIELEFETDEKMPKIYGNPTALGILIRNLVDNSIRYSMENGRVIVRLLKHN
                     NEIILEVCDNGPGIPPELQKRVFERFFRVLGNKSPGSGLGLAIVQQICELHGGRVVLD
                     SPKIGTGLIVRVYLPIKTKPK"
     gene            complement(3095..3415)
                     /locus_tag="VAH76_001746"
     CDS             complement(3095..3415)
                     /locus_tag="VAH76_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386363.1"
                     /translation="MKRMIGFMVSVVLTFAVNAADSQVNTHKHQTDKGKVTPGMSIQR
                     NTEQSTAITADAASSNTTEKVTSQQMKEHKNSVKEANYNAQKKEEEAKTATDTQKHEA
                     DSPS"
     gene            complement(3467..4066)
                     /gene="ruvA"
                     /locus_tag="VAH76_001747"
     CDS             complement(3467..4066)
                     /gene="ruvA"
                     /locus_tag="VAH76_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO1386364.1"
                     /translation="MIGWLHGQIIDKHQPGKLVLDVNGVGYDVETSLNTFFQIENGNQ
                     PIGLHIHTIVREDALLLYGFLDKEERSLFRSLIKVNGVGPKLAMTVLSSISPKEFIQC
                     IHQENAALLTKLPGIGKKTAERLVVEMRDSIKQFDGSVSDTFQKQAGSTHSQQEAISA
                     LEALGYKPQEAWKVVNKIDNGNKSCEQLIREALQILSSR"
     gene            complement(4063..4587)
                     /gene="ruvC"
                     /locus_tag="VAH76_001748"
     CDS             complement(4063..4587)
                     /gene="ruvC"
                     /locus_tag="VAH76_001748"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEO1386365.1"
                     /translation="MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDAELSH
                     KLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRAKICE
                     YSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
                     LIGSRGTKRRRMRL"
     gene            complement(4587..5330)
                     /locus_tag="VAH76_001749"
     CDS             complement(4587..5330)
                     /locus_tag="VAH76_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO1386366.1"
                     /translation="MAGHSKWANIKFRKGVQDAKRGKIFTKLIREITVAARMGGGDES
                     SNPRLRDAVKKALNANMKRDTIDNAVKRGVGGADGEAMIAMRYEGYGPGGVAILVDCL
                     SDNKNRTVSEVRHAFSKHGGNLGTDGSVSYLFTNQGEILMASNQPEDKVMEIAIDAGA
                     SDVAVEDSQVEIITPVEAYHTVLNALQDAGLEVEQSHLTMRAQTLVPISDETAESLIK
                     LIDMLEDLDDVQEVYSNAEFSEKILESMN"
     gene            5478..6728
                     /locus_tag="VAH76_001750"
     CDS             5478..6728
                     /locus_tag="VAH76_001750"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEO1386367.1"
                     /translation="MYLQGFGNYHHSEAVKGALPPNQNSPQHCNLGLYAEQLSGTAFT
                     RPRHNNLRSWLYRILPTVTQGTYYPYEFNIMHSFVDEFSPNAMRWSPLYNSSQIKCDF
                     VEGLFHIAGSPLVNTYTYYCNHSMSDKYFANNDGELLFVPYAGEIHLYTEFGKLMLSS
                     GSIAVIPRGVKFKVEVISKEAKGYLCENSGNPLTLPQLGPIGANGLANPRHFQYPVAA
                     FEDSGGEHTIICKNQKKLWFTVCNHSPLNVVAWHGNYAPYCYDLSLFNTINTVSFDHP
                     DPSIFTVLTSESEIPGVSNLDFVIFPPRWMVAEHTFRPPYFHRNYMNELMGLVYGEYD
                     AKKEGFIPGGISIHNCMTPHGPDYESYEIAASQDLKPNYINSLAFMFETKDYWQVTEQ
                     AYRHPSRQVDYLNCWQGFKVEFSQ"
     gene            complement(6828..7853)
                     /gene="rpoS"
                     /locus_tag="VAH76_001751"
     CDS             complement(6828..7853)
                     /gene="rpoS"
                     /locus_tag="VAH76_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEO1386368.1"
                     /translation="MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLD
                     EFPEFTDDEVFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
                     ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
                     WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKP
                     LEDVQKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLL
                     DKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVG
                     LTQEDLF"
     gene            complement(7939..8682)
                     /locus_tag="VAH76_001752"
     CDS             complement(7939..8682)
                     /locus_tag="VAH76_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO1386369.1"
                     /translation="MMRLFRKQLICIFFILSVAGCGTRSDLAPVTELKWQPYSKYQKF
                     HTVRSGETLYAIAFRYDTDYRRLAIINHLRPPYSLRVGQVLSLKGIVPKSNHFHSAPR
                     ANLHITHKPKQKIIYSPAYNVRSSSGWLWPVTGRVVTTFIPSQGKKGINIACNRGDKV
                     RASASGVVAYAGSGLPGYGNLIIIKHSNEYLTAYGNNARNLVTEGQRVSAGQVIAEAG
                     LIDRSYWGVHFEIRRAGVPVNPLNYLQKG"
     gene            complement(8701..9459)
                     /gene="surE"
                     /locus_tag="VAH76_001753"
     CDS             complement(8701..9459)
                     /gene="surE"
                     /locus_tag="VAH76_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEO1386370.1"
                     /translation="MKILVSNDDGVLAPGIKILANELSTLGEVKVVAPDRNRSGASNS
                     LTLTQPLRVKQLDNGYYSVDGTPTDCVHLALTGFLEPIADIVVSGINEGANLGDDVLY
                     SGTVAAAMEGRYLGLPAIAISMVGDNIQHYETAAIIAKQLVIKLSANKLPSQTILNVN
                     VPDLPLNQIRGMQVTRLGTRHSAEPIIKEYDPRGRPIYWVGPPGIEADAGAGTDFFAI
                     KTGHVSITPLHLDMTHYKLFDHLSNLLNEICIEN"
     gene            complement(9462..9845)
                     /locus_tag="VAH76_001754"
     CDS             complement(9462..9845)
                     /locus_tag="VAH76_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386371.1"
                     /translation="MNKYLVIILITLGLVSCQFKKDESYYRSHPSELQNALKLCPGTQ
                     PDGLSCQQLEAIGRRMNSLAYQLQYNPQEFGNKILALQQVIANQQREIQSKKDNAELQ
                     DSLKRNQEDLAYYLAVVKWLESPES"
     gene            9925..11649
                     /locus_tag="VAH76_001755"
     CDS             9925..11649
                     /locus_tag="VAH76_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEO1386372.1"
                     /translation="MLDFSLNRDPQTGELYIETSLCGKPLLTTPQLNKGTAFTQEERK
                     DFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHL
                     GEMLPIIYTPIVGAAAKRFSHEYRQPRGLYIAHSDKNQLEEIINNRSNPDIDLVVVTD
                     GEGVLGIGDQGIGGMDIPVAKLMVYSLCGGIDPTRTLPIFLDVGTNNQELLNDPMYLG
                     CRHPRIKSSEYDDFIKTFVNEIHKQFPNAFLHWEDFGRGNARRILDQFQDELCTFNDD
                     IQGTGAVTLAAILAACDVTGLPLHEHRIVVFGAGSAGTGISDQIVDAMVKSGLSLTDA
                     YDRFWLIDKQGLLLATDQELTDAQKPYARNPIDIQSWEINNKQHPSFTDTIRHVKPTI
                     LIGCSAQTGAFSQDIVETMSTACERPIIFPLSNPDEKCEAQPSDILAWSKGRALIATG
                     TAFAPIEYQNRMVQIAQCNNALVFPGIGLGVLAVSASRLTKDMILAAAQTLSKFAPSK
                     KDSFLPLLPSLDNAQIVAKEIAIAVAQCAIDSGYAQKNQDKDLPRLIDELFWEPRYLP
                     FRKKGWSE"
     gene            complement(11697..12068)
                     /locus_tag="VAH76_001756"
     CDS             complement(11697..12068)
                     /locus_tag="VAH76_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEO1386373.1"
                     /translation="MKKLFSGLLMGFSALAYANNDVVISVSSKNPSFVVSLAANPTTG
                     YQWSVVEFDKNLLTLSNSIYEKPKTNLIGAGGKMLFTFNLNKGKTYPAQTKMVFKYAR
                     SWEPESGTIKNVTINFTNPSD"
     gene            complement(12105..12422)
                     /locus_tag="VAH76_001757"
     CDS             complement(12105..12422)
                     /locus_tag="VAH76_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEO1386374.1"
                     /translation="MIIRNYLLAIVFLMIVYPNAYAMRCGDKLVYEGDSKYTVLSKCG
                     EPLDKLVYEENVPLYNAAGYQIGYGTNTVETWIYQKSPVDFQYELIFDYGKLKQINVN
                     RNP"
     gene            complement(12505..14316)
                     /locus_tag="VAH76_001758"
     CDS             complement(12505..14316)
                     /locus_tag="VAH76_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1386375.1"
                     /translation="MNSYNKKSEELPKTLGRFFWHFIKKQPLAFTVFFVAPIAMVLEN
                     NAIPYSLKMIIDALGGHSGEENIFSAVAPALWLGGGAWFALIVILRLQNWWQGYVIPR
                     FQADVRMSVMEYLSRQSYHYFSNQMAGGLANKVNDLPRALDSIFNIITWYAIAAFASI
                     IVALILMCTINYWFAVILLSWIIFQLYISYRLSKKVDQYAKENAEDKSQLSGKIVDSL
                     SNASVVKLFARSRDELAYISASQNMEVQSNKKLSIYMNIFRLYLDIPVTVMLVAMVYF
                     LLTFWNKKLITTGDLVFIFNVSFSIMTQIWYLCHAIAEFFREIGIARQAIALLSAPIE
                     VEDAPDAKPLIVKEGKIEFDSVTFHYNQGKKVFENKSVIIKPRQRVGLVGFSGSGKTT
                     FIHLILRLFNVESGKILIDGQDISQVSQDSLRSAISLIPQDTTLFHRTLMENIRYGNP
                     DASDDEVVMASKQACCDDFISLLPDGYNTLVGERGIKLSGGQRQRIAIARAILKNTKI
                     LILDEATSQLDSLTEEAIQNSLWQLMESKTTIVIAHRLSTLLHMDRILVFEAGKIIED
                     GSHEELLAKDGLYKSMWDAQVGGFLPESDRDRDNWDE"
     gene            14552..16183
                     /locus_tag="VAH76_001759"
     CDS             14552..16183
                     /locus_tag="VAH76_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEO1386376.1"
                     /translation="MEHETMEFDVIIVGAGPSGLSAAIKLKQLALASQKEITICILEK
                     GAQVGAHILSGAVLEPRSLKELLPENWQQAPLDTPVNKDLFYFLTQNKSFKLPTPKPM
                     HNEGNFIISLGELCKFLAEQAEALGCEIYPGFAAAEILYNDKNQVIGVATGDVGIDRQ
                     GKKTDNYQPGMHLLAKQTLFAEGCRGQLSQTLMSRYHLRDHSQPQTYGIGIKEIWQVD
                     ASRHEPGKVIHTVGWPLDHATYGGSFLYHLSEQRVAIGFVVGLDYKNPWLNPFGEFQR
                     FKTHPLIKSVLTGGERIGYGARALNEGGWQSLPKLTFPGGALIGDAAGFLNVPKIKGI
                     HTAMQSGMLAAQACFDALSEEKNGQFELSAYSEKINQSWLKQELYAVRNIRPGFKYGL
                     FPGLINAALETYVTRGYSPWTLKNHADHKSLIPAEKAKKINYPKPDGVLTFDKLSSVF
                     LSNTFHEENQPCHLKLKQPKLAIEVNLNQYASPESRYCPAAVYEIIENVEKGPQLQIN
                     AQNCIHCKTCDIKDPRQNIVWQAPEGGGGPNYSGM"
BASE COUNT         5063 a         3342 c         2909 g         5016 t
ORIGIN      
        1 tttgatcatt attgccaaaa aacaccaaac aaaagtgaat actattatca gtttaaatcc
       61 aggacttaaa aaatcaatgc cccttcgctt gggacaaaaa atattaattg gctagattat
      121 gtgcattatc gtgatataaa ctagatagat taatgcatct gtttgaaaca gaaacgtgct
      181 tttgagggaa taccaagact actggcatgc cgctaaaagc ttaaagatta ttaaataaaa
      241 aatctgtgat aagtatgtaa attctgcaaa ttattttgtt aatacggtat gaagcttgca
      301 ggtgagttct aaaacttgtg ttataatgat attaattaat tgataaatta aacaatatat
      361 gaaattacta ttcgattttt ttcctatcgt tttatttttt atagtctata aattttttgg
      421 catttatacc gctactgctg ttgctatggt agcttcatta actcaagttg cgttttacag
      481 attaaagttt cagcattatg aaaaaatgca tttgtttagt ctggctatca ttatggtctt
      541 gggaggagcg acgttattct ttcaaaaccc ttggtttatc aaatggaagc ctacaggaat
      601 ctactggtta tctgcacttg tcttttatgg ttcaggttac atagggtcta agccactaat
      661 ccaaaaaatg atggaagcaa acatcaattt aacaacaaaa atttggtatc gacttaattt
      721 ggcatggaca ttatttttta tagtaatggg ggcattaaat ttatatgttg cctatcatta
      781 tgacactgat gtatgggtta attttaaatt atttggtggt gttgggttta ctttactttt
      841 tgtgttgatt caagcttttt atttaacaaa acacacagat gaaaaaagct ttgaaaaaca
      901 atagtaacat ctctgtatct gcagaaaaat atagcatgta ataagagaaa aagtaggagc
      961 accgatgaga ttattacttg ttgaagacga tgaactgctt ggcgatgcag taaaagccgg
     1021 actgacacaa tttggttacg ttgttgactg gctaaaagat ggcgaagctg ctcgggcagc
     1081 tctcaaatca gagtcttttg aattaattat tcttgacttg ggattaccta aattatccgg
     1141 tttatcatta ttgcaaaaca tcagacatga tggaaatagc acacctgtga ttattcttac
     1201 agctcgggaa tcaatagaag atagagtaaa aggcttagat agcggtgctg acgattatct
     1261 tattaaacct tttgatttaa acgaattaag tgcaaggatt agagcattgg taagacgctc
     1321 tcagggcaga gctgactccg ttttgcaata taaaaatatt actctcgatc ctgctgctca
     1381 ttctgtattc gtggatgatg tactcgtcaa tgttccaaga agagaatttg ctttattgca
     1441 gaaattactt gaaaacagtg gccaggtttt atctcgagaa caattaatgc agagtattta
     1501 tggctgggaa gaagacgtgg acagtaatgc tctcgaagtt catatccaca atttacgtaa
     1561 aaaacttaac gcaaatttta tccgcactat acgtggtgta ggatatatgg ctgaaaaaaa
     1621 tgacagtgta cttctatcgt gaaatcgtcc attcgaaaat ttttattaat taatctgctt
     1681 cttgcaatta ctattactac aacattaact gctattggta actattatct tgatcaaaaa
     1741 gacattcaag accaccttga tacattgatg gctatttccg cgctatccta tcaagcctta
     1801 ttaggagatg atcttcatca aagacctctt gcaaaaattc aaaatgccct ggaatctatt
     1861 ccccaaaaaa ttaatactta ttatcaaaaa agatatttaa atgatgagcc tcaagaaaat
     1921 tatttggata aatttaattt tcaggtatgg accaatggag gtaaattatt attacattca
     1981 cctactgccc ctaaaatacc attaacagca gagacagacg gcttcagcga taaaagaatt
     2041 tctaaccagg attggcgagt attcacaacc tataatgaca aagcaggaat tcgtactgta
     2101 ctggcagagc gttatgatac cagaaatgag ctgggtcaca gaattgccca agatgatctc
     2161 tatattatgt tgcttacttt tccattatca ggactattga tatggatcat tattggaaga
     2221 ggactagaca gtctggatag agtagcagaa gaggttgcta accgtgcacc aagccacctg
     2281 gaacctgttg atttacaaga agttcccgaa gaaattaaac ctgtaattga tgaattaaat
     2341 aaattatttt ttcgactcaa agaagggttt gagcgtgaaa aacgatttgc tgccgatgca
     2401 gcacatgagt tgagaacacc tcttgcagca cttaaggccc aagctcaagt ggctttgaac
     2461 tcgaatgata tagaagaaaa aaatcaggct ttacaaaaat taattgccag cgttaatcga
     2521 agcacacata tagtgcagca gttgctaacc atgagtaaac ttgtaccaga agcaacagaa
     2581 ctacatgagc aagatgaagt caatctaagt aaactaactc gtgaaatatt agccatgttg
     2641 gccccgtctg cggtcgaaaa acaaattgag ctggaatttg aaaccgatga aaaaatgcct
     2701 aaaatttatg gaaatccaac ggctttggga atattaatac gcaatctggt tgacaattcc
     2761 attcgatatt ccatggaaaa cgggagagtt attgttcgat tattaaaaca taataacgaa
     2821 atcatactcg aagtatgtga taatggccca gggatcccac ctgaattaca aaaacgtgtt
     2881 tttgaaagat tttttagagt attaggaaac aaaagcccgg gtagcggtct gggtttggct
     2941 atcgtgcagc aaatatgcga acttcacggt ggacgggtcg tgcttgattc gcctaaaatt
     3001 ggcacaggcc taattgtgag agtttatttg cccatcaaaa caaaaccaaa ataaactcaa
     3061 taatcaaatc ataaccttgc tgtaattaat ctttttagct tggagaatct gcttcatgtt
     3121 tctgtgtgtc ggtcgcagtc ttcgcttctt cttccttctt ttgagcatta tagttcgctt
     3181 cctttacact gtttttatgc tctttcatct gttgggaagt aaccttttcc gtggtatttg
     3241 aagaggctgc atcggctgta atagccgttg attgctctgt gtttctctga attgacatac
     3301 ctggggtcac tttaccctta tcagtttggt gtttatgagt attgacttga gaatcagcag
     3361 cattgacagc aaaggttagc accacactca ccataaaacc gatcattctt ttcataaatg
     3421 ctcctgttta ttatcgagaa ttttatcaga tcgcgcagtt acaatctcat cgcgaagaaa
     3481 gaatttgcaa agcctcacga atcaattgct cacaactctt attgccatta tcaattttat
     3541 tcacaacttt ccacgcctct tgtggcttat agcctaatgc ctccaacgcg ctgattgcct
     3601 cttgttgact atgagtacta cctgcctgct tttggaaagt atcggaaact gaaccatcaa
     3661 attgctttat actgtctctc atttcaacta ctaggcgctc tgcagttttc ttgcctatgc
     3721 caggtaattt ggttaataaa gcagcattct cctggtgaat acactgaata aattccttag
     3781 gagaaatact ggataaaaca gtcatagcca gtttggggcc taccccattc actttaatta
     3841 atgatctgaa taaagaacgc tcctctttat ccaggaagcc atacaataac aaagcatctt
     3901 ctcgaactat ggtgtgaata tgtaatccga taggttggtt accattttct atctgaaaaa
     3961 aagtattcaa agatgtttct acatcatatc ctactccatt gacatcaaga accaattttc
     4021 ctggctgatg cttatctata atttgaccat ggagccagcc tatcatagtc tcatcctcct
     4081 acgtttcgtt cctcttgaac cgattaattt tgacaaacca tttctcatat ggctatgaca
     4141 tattgctatt gccaaagcat cggcagcgtc tttttgagga gctcgattga gctgaagaag
     4201 ctctacaacc atatgactta cttgatcttt ttcagcggcc ccataaccaa ctacactttg
     4261 ctttatttct cttgctgaat actcacagat tttcgcacga tgtgaggctg ctgctaccat
     4321 ggcaaccccc cgcgcatgtc ctaatttcaa agctgaatta ggattgttat gcataaatat
     4381 ctgctcaata gcaacctcag ttggggaata gtgatccatt agctcacata ttccatcata
     4441 tatttgcaat aatttatgac ttaattccgc atcgtttgat gtgcgaatac agccgctatc
     4501 gatgtacgcg attttacggt cagattcttt aatcaatcca tatcctgtaa cccgtgagcc
     4561 tggatctatc cctaatataa tcgtcattaa ttcattgatt ctaatatttt ttcagaaaat
     4621 tcagcattgc tgtaaacttc ttggacatca tccaaatctt ccagcatatc aatcagttta
     4681 attaaacttt cagccgtttc atcactaatt ggaaccaagg tttgtgcacg catagttaaa
     4741 tgagattgct caacctccaa accggcatcc tgtaaagcat ttaagacggt gtgataagct
     4801 tctacagggg taatgatctc tacctgactg tcctctacag caacatcaga agcacctgca
     4861 tcgattgcaa tctccatcac cttatcttcc ggctgattag aagccatgag aatttcacct
     4921 tgattagtga aaagatagga taccgagcca tccgtgccca aattaccccc atgttttgaa
     4981 aaagcatgcc gaacttccga taccgttcga tttttattgt cagacaaaca atctaccaat
     5041 atagcaaccc cacctggccc gtaaccctca taacgcatag ctatcattgc ttcgccatca
     5101 gctcccccca cccctcgctt gacagcatta tcgatggtat cgcgcttcat attggcattc
     5161 agtgcttttt taacagcatc tctcaaacgt ggattacttg actcatctcc tcctcccatg
     5221 cgggcagcta cagtaatttc acgaattaat ttagtaaata tttttcctct tttggcatcc
     5281 tgtacgcctt tacggaattt gatattagcc catttactat gaccagccat tacatcacct
     5341 ctaaatgaat ttttattagg agattataca atgctctaac atcaaaaaga atgactaaag
     5401 cgaacgatgc tctcgacaat ttgaactttt ttggactatc ctagcaccat caaaaaaatc
     5461 ttgaaggaga ttgaactgtg tatttgcaag gatttggtaa ttatcaccac agcgaggctg
     5521 ttaaaggagc attaccccca aatcaaaact cacctcaaca ctgtaactta ggactttacg
     5581 cagagcaatt gagtggaacc gcattcaccc gtccccgaca taataatctt cgaagctggc
     5641 tatatagaat acttcctact gttactcagg gtacctatta cccctatgaa tttaatataa
     5701 tgcattcttt tgttgatgag ttctcaccca atgccatgcg ttggtcacct ctttataaca
     5761 gttctcaaat taaatgtgat tttgttgaag ggctatttca tattgccggt agcccgttag
     5821 tcaataccta tacttattat tgcaaccatt ccatgagcga taaatatttc gccaataatg
     5881 atggtgagtt attatttgtt ccttatgcag gcgagattca tctgtatact gaatttggca
     5941 aattaatgct ctcttccgga tcgatcgcag tgatacctcg tggcgttaaa tttaaagtgg
     6001 aagtaatcag caaggaggca aaaggttatc tttgcgaaaa tagcggaaat cccttaacct
     6061 tacctcaatt aggccccatt ggagccaatg gtttggcaaa cccacgacat tttcaatacc
     6121 cagtagccgc atttgaagat tctggcggcg agcatactat aatctgtaaa aaccagaaaa
     6181 aattatggtt tactgtatgc aaccactctc ctttaaatgt cgtggcctgg catggcaatt
     6241 atgcaccata ttgttatgat ctcagtttgt tcaatacaat taacacagtc agtttcgatc
     6301 accctgatcc ttccatattc actgtattaa cttcagaaag cgaaataccc ggtgtttcta
     6361 acttggactt tgttattttc ccacctcgct ggatggttgc cgaacatact tttagaccac
     6421 cttattttca cagaaactac atgaatgaac tgatgggact tgtctatggt gaatatgacg
     6481 ccaagaagga aggattcata ccgggcggta tcagcatcca taattgcatg actccacacg
     6541 gccctgatta cgaatcttac gaaattgctg catcgcagga tctaaaacca aattatatca
     6601 actccctggc ctttatgttt gaaaccaaag actactggca agtaactgag caagcttata
     6661 ggcacccaag caggcaagtg gattatctta attgttggca aggctttaaa gtagagttta
     6721 gtcaataaat tgactgcttt ttaagatgta aaattgacgg aaattaatta atttggcttc
     6781 agttgggtca atttcgaccc aacttgcata tagttaacta ggttaaatca aaacaaatcc
     6841 tcttgtgtta gtccaacccg ctctaacaaa gttctaagtg tcttcaaagc ttcaacttga
     6901 atttggcgaa cgcgttctcg ggtcaaatca atttccttac caacgtcttc cagagtcgct
     6961 ttttcaaaac ctcttaaccc aaaacgtctt gcgataacct gctgttgatt ctcagttaat
     7021 ttatccagaa gagattctat atgctgcctt aaattctcat tggttagcag ctctgcagga
     7081 tttaaactgt tttcatcagc aattgtatct aataaagatt tggtttcatc aaatccaatt
     7141 ggagtatcaa ctgaagccac tttatcattc aatcccaata atttttggac atcctccaaa
     7201 ggcttatcca ccatttctgc tatttcttca ggtgatggtt catgatccag tttttgcgtc
     7261 aattgtcttg ctgctctgag ataaacatta agttctttca cgacatgaat aggcaagcgg
     7321 atagttctcg tttgattcat aattgcgcgc tcaatagttt gcctaatcca ccaggtcgca
     7381 taagttgaaa aacggaatcc tcgcttgggg tcaaatttct caacagactt cattaaaccc
     7441 aggttaccct cttcgattag atccagaaga ggtaatcctc tattaagata acgtctggct
     7501 attttaacca ccaggcgcaa attcgactca atcattttct tgcgtgcttc catatcacct
     7561 tttaaagcaa gagtcgcata atggatttct tcttcagccg ttaacaaagg agaaaagcca
     7621 atttcaccca agtaaagttg agtcgcatct aaagccttag caatgttttt cccgcgctga
     7681 aatgaaggaa aaacttcatc atcagtaaac tctggaaatt catcaagatc ttcttccatt
     7741 ttttcgatat caggttcaac atcttcttct gacaacagag agtcatcatc tggctcagac
     7801 cattcctctt ctttaaattc cttgtcctta attggctctt cttcatcttg cattgtatca
     7861 cctatatgtt tagctatgcc ttgaaatagt tttttacttc ttaacaagcc tattgttgct
     7921 ataactatga gttatatgtc aacctttttg tagataattc aatggattaa ctggcacccc
     7981 tgctcttcta atttcaaaat gaacacccca gtatgatcta tctattaaac cagcttctgc
     8041 tattacctgc cccgcactaa cacgctgccc ttctgtcacc agatttctgg cattgttacc
     8101 gtatgcagtt aaatattcat tactgtgttt aataatgatt aaattaccat acccaggcaa
     8161 accgctgcca gcataagcca caactccact cgctgaagct ctaactttgt ctcctctgtt
     8221 acatgcaata tttatgcctt ttttcccctg agaaggaatg aatgttgtaa caacacgacc
     8281 agtaactggc cacagccaac cagaggaaga acgtacatta taagctggag aataaataat
     8341 tttttgtttt ggcttatggg taatatgtaa gttagctctg ggagctgaat gaaagtgatt
     8401 tgattttggc acgatgcctt ttaaacttaa cacttgtcct actcgcaagg aataaggcgg
     8461 ccttaggtga ttgataatcg caagccgccg ataatcagta tcataacgaa atgctatagc
     8521 ataaagggtc tcacctgatc gcaccgtatg gaatttctga tattttgaat aaggctgcca
     8581 ttttagctcg gtaacaggag ccaaatcact tcttgtccca caaccagcaa cacttaagat
     8641 aaaaaaaata caaattaatt gtttgcgaaa taggcgcatc attatctcat ttattattta
     8701 ttaattttct atgcaaattt cgttaagcaa gttagataaa tgatcaaata acttataatg
     8761 agtcatgtct aaatgaagcg gtgttataga aacatgacct gttttaattg caaaaaaatc
     8821 agtgccagca ccagcatctg cttctatgcc cggtggacca acccaataaa ttggccttcc
     8881 tctgggatca tattctttaa taataggctc agcgctatgg cgagttccca atcgcgtaac
     8941 ctgcattccc cttatttgat tcaaaggtaa atcaggaaca ttaacattta atatagtttg
     9001 agaaggtaat ttgttagcac ttaattttat aaccaattgt ttggcaataa ttgcagctgt
     9061 ttcataatgt tgtatgttat ctcctaccat tgagattgca atagcaggca accctaaata
     9121 acgaccttcc atagccgctg ctactgtacc tgagtaaagt acatcatccc ccaaattggc
     9181 accctcatta atccctgaaa cgactatgtc tgctataggc tctaaaaatc cggttaacgc
     9241 cagatggacg caatcagtag gtgttccatc cacactataa taaccattgt ccaattgttt
     9301 aactcttaaa ggttgtgtta aagtcaaaga attactggcg ccacttctat ttctatcagg
     9361 cgcaaccact ttaacttctc ctaaagtgga cagctcatta gctaaaattt tgatcccggg
     9421 cgctaaaacc ccgtcatcat tgcttactaa tattttcatg tttaactctc aggggattct
     9481 aaccatttta caactgctaa atagtaagca agatcttctt gatttctttt caatgaatct
     9541 tgtaattcag cgttatcctt tttactctgt atttcccttt gctgattggc aataacctgc
     9601 tgcaaagcca aaattttgtt accaaactct tgtgggttgt attgtagttg ataagcaaga
     9661 ctattcatcc tcctgccaat tgcctccagt tgctgacaac ttaatccatc gggttgcgtg
     9721 ccagggcaca attttaaagc attttgcagt tcggatgggt gggaacgata ataggattcg
     9781 tcttttttaa actgacatga aaccaatccc aaagttatta atattatcac taagtactta
     9841 ttcatacccg ccctcttgat gtaaactaca aaaagtcgat attatcacca gtcagcaagt
     9901 aacgctatct tatgagaata ccatatgctt gattttagtt tgaatcgtga tccccaaacg
     9961 ggcgagttat acatagaaac ctcattatgt gggaagcctt tactgactac gccgcaatta
    10021 aacaaaggaa cagcatttac tcaggaagaa agaaaagatt ttggtctttt aggcaagtta
    10081 ccacaccgcg tagaaactct tgatgaacaa gtaaagcgtg cctatcttca atattcaagt
    10141 tacacgacac gtttgcagca acatatttat ttaaataatt tacatgataa aaaccaaata
    10201 gtattttaca aactgctaag tcgtcatttg ggggaaatgc ttcccatcat ctatacacca
    10261 atcgttggtg ctgcagccaa gaggtttagt catgaataca gacagccacg aggattgtat
    10321 attgctcatt cggataaaaa ccagcttgag gaaataatta ataaccgttc gaatccagac
    10381 atagaccttg ttgtagtgac cgatggtgag ggagtcctag gcatcggcga tcaagggata
    10441 ggaggaatgg atatacctgt tgcaaaacta atggtttaca gcttatgtgg tgggattgat
    10501 ccaactcgca cattacccat atttcttgat gtaggaacta acaaccaaga attattaaat
    10561 gaccctatgt atctgggttg ccgtcacccc agaataaaaa gctcagaata cgacgatttt
    10621 atcaaaactt ttgtcaatga aatccataag caatttccaa atgctttttt gcattgggaa
    10681 gattttggtc gaggcaatgc tcgacgaatc ctggatcaat ttcaagatga attgtgtact
    10741 tttaatgatg atattcaggg aactggtgca gtaacactgg cagcaatttt ggcagcatgt
    10801 gatgtgactg gactaccctt acatgaacac agaatcgttg tgtttggagc tggctcagca
    10861 ggtactggta ttagcgacca aattgttgat gcgatggtga aaagcggatt gagcctaact
    10921 gatgcttatg acagattctg gttaatagac aaacagggtt tattacttgc gactgaccaa
    10981 gagcttactg atgctcaaaa accctatgca cggaatccta tagatataca atcctgggaa
    11041 attaataata aacaacatcc ttcgtttact gacactatca gacatgtcaa acctaccatt
    11101 ttaattggct gttcagcgca aacgggcgca ttttcacaag atattgtgga aactatgtcg
    11161 acagcctgcg agcggccaat cattttccct ttatcaaatc ctgatgaaaa atgtgaagca
    11221 caaccttcag atatattggc atggagtaaa ggacgggcat taattgctac tggaaccgct
    11281 tttgccccga ttgaatatca gaaccgaatg gtgcaaatag ctcaatgcaa caatgccttg
    11341 gtattccctg gaattggatt gggtgtcttg gcggtaagtg cctctcgttt aaccaaagat
    11401 atgatattgg ctgccgctca aactttaagt aaatttgcgc caagtaaaaa ggacagtttt
    11461 ttgccccttt taccctcatt agacaatgca caaattgtag ctaaagaaat agctattgcc
    11521 gtagctcagt gtgcaataga ctcaggctat gctcaaaaaa atcaagacaa ggatctaccc
    11581 cggttaattg atgaactctt ttgggagcct agatatctgc cttttagaaa aaaaggatgg
    11641 agtgaataga ataaagctct atgactatat tattgagaaa taaattttct taattcttag
    11701 tctgaggggt tggtgaaatt aatcgttaca ttttttatag tcccgctctc tggctcccag
    11761 gaacgagcgt atttaaagac cattttggtt tgagcgggat aggttttacc tttattcaag
    11821 ttaaaagtaa atagcatttt accgcccgca cctatcaaat tagtcttggg cttttcataa
    11881 atgctattac ttaaggtaag caaatttttg tcaaattcta ctacagacca ttgataaccc
    11941 gtagttggat tagctgcaag actaacaacg aaactgggat tttttgagct gacactgatg
    12001 acaacatcgt tatttgcgta agccagagca gagaaaccca taagtaatcc gctgaataat
    12061 tttttcatat tttttccctc acaaagcttc ttacacgctc accattatgg gttacgattt
    12121 acgttaatct gttttaattt cccataatca aaaattaatt cgtattgaaa atcaacagga
    12181 gatttttgat aaatccaggt ttcgactgta ttagtaccat aacctatctg ataaccagcc
    12241 gcgttgtata atggcacatt ctcctcatag actaatttat caagtggctc accacattta
    12301 gacaaaacag tgtatttact atccccttca tagaccaatt tatctccaca ccgcattgca
    12361 tacgcattag gatatacaat cattaaaaag acaatagcca acaaataatt ccggatgatc
    12421 attccctacc ccttgagaac tcaatgctcc ttgcaacaaa attttcaaaa agtctatttt
    12481 tatagcatag cacttggtat ctgattattc atcccaattg tctctgtctc gatcggattc
    12541 aggcaagaat ccacctactt gagcatccca catggattta tataaaccat ccttcgctaa
    12601 taactcttca tggcttccat cttcaattat ttttcctgct tcaaaaacta aaattctatc
    12661 catatgcaat agtgttgata atcggtgagc aataacaatt gtagtctttg attccatgag
    12721 ttgccacaat gaattttgta tggcttcttc ggttagggaa tccagctgtg aagtagcctc
    12781 atctaatatc aaaatcttcg tattttttaa aatagcgcga gcaattgcaa tgcgttgtct
    12841 ttgccctcct gatagtttaa ttcccctttc acccaccaaa gtattatagc catcgggtaa
    12901 aagtgaaata aaatcgtcac agcatgcctg ttttgatgcc atcacaacct catcatcact
    12961 cgcatcaggg ttaccataac ggatgttttc cataagagtc ctatgaaaca atgtagtatc
    13021 ttgagggata aggctgatag ctgaacgtaa ggaatcttga gacacttggc tgatgtcctg
    13081 accatcaatc aaaatttttc cagattccac attgaatagg cgtaaaatta aatgaataaa
    13141 ggtcgtttta ccgcttcccg aaaaccccac caaaccaaca cgttgtctag gtttaatgat
    13201 cactgattta ttttcaaata ctttcttacc ctggttataa tgaaaagtta cgctatcaaa
    13261 ctctattttt ccttctttaa caatcaatgg cttggcatca ggagcatctt ctacctctat
    13321 cggtgcacta agcaaagcaa tagcttgtct tgctatgcct atctctcgaa agaactcggc
    13381 aatagcatgg cacaaatacc aaatttgagt catgattgaa aacgatacat tgaatataaa
    13441 gaccaaatct cctgttgtaa tgagcttttt attccaaaaa gtcaacaaga aatacaccat
    13501 agccactaac atcactgtga ctggaatatc gaggtacaaa cgaaatatgt tcatataaat
    13561 cgacagcttt ttattgcttt gtacctccat attctgactt gcggaaatat aagccagctc
    13621 atcgcgagaa cgagcgaaaa gcttaaccac actggcatta ctcaaactgt caactatttt
    13681 accgcttaac tgacttttat cctctgcgtt ttcttttgca tattgatcaa cttttttaga
    13741 taatctatag ctgatataaa gctgaaaaat aatccagctt aacaaaataa ctgcaaacca
    13801 gtagttaatg gtacacatca aaatcaatgc cacaatgata gaagcgaatg ctgcgatcgc
    13861 ataccaggtt atgatattga atatggaatc aagagcacgg ggtaaatcat tgactttgtt
    13921 agctaaacca cccgccattt gattggaaaa ataatgatag gactggcggc tcaaatactc
    13981 catgacagac attctgacat cagcctgaaa tcttggtatc acataacctt gccaccaatt
    14041 ttgcagacgt aaaatcacaa ttaaggcgaa ccaggcacct ccacctaacc ataaagccgg
    14101 agcaactgct gaaaaaatat tttcctcccc ggagtgcccc ccaagagcat caatgatcat
    14161 tttcaaagaa taaggaatag cattattttc aagcaccata gcaatcggtg ctacaaaaaa
    14221 cacagtaaaa gccaaaggct gtttctttat aaaatgccag aaaaaccgac ccagtgtttt
    14281 tggcaactct tcagattttt tgttatagct attcataaac cataccttca atgaattttg
    14341 acaaggttcg attattctaa cctaatattt gctaattgga gaatttatgg ttaaaaaaca
    14401 attatatgcc ctaccaattc acctattctc tcagatttac attgccatga aataaaattc
    14461 gcccttcaaa aattgaaaat ataaaattta atcttgatct taaaccttat aaaggcgatc
    14521 attttttcat ttgtaagtta gaggagtacg tgtggaacac gaaacgatgg aatttgatgt
    14581 tatcatagtc ggtgcagggc cttctggatt atccgctgca atcaaattaa agcaactggc
    14641 tttagcttca caaaaagaaa ttactatttg tatactggaa aaaggagctc aggtcggcgc
    14701 tcatattctt tctggtgcag ttttagaacc cagaagctta aaagaattac tgccagagaa
    14761 ttggcaacag gccccgctag atactcctgt taacaaggat ctgttttatt ttttgactca
    14821 aaacaaatca tttaagctcc ctactccaaa acctatgcat aatgaaggaa atttcattat
    14881 cagtttggga gaactatgta agtttcttgc agaacaagca gaagctctag gctgtgaaat
    14941 ataccctgga tttgcggcag cagaaattct ctataatgat aaaaatcaag tcataggtgt
    15001 agcgaccggg gacgtaggaa ttgatcgaca aggaaaaaaa actgataatt atcaacctgg
    15061 catgcattta ttagccaaac aaactttatt tgctgaggga tgtcgcgggc aattaagtca
    15121 aacattaatg agtcgctatc atttaagaga tcattcacaa ccacaaactt acggtatagg
    15181 gattaaagaa atctggcaag ttgatgcatc ccggcatgaa cctggaaaag tcatccatac
    15241 tgttggttgg cctcttgatc atgcgactta tggaggctcc tttttgtatc atctttcaga
    15301 gcagagagtt gctataggtt ttgttgtagg acttgactac aagaacccat ggttaaatcc
    15361 ttttggagag tttcagagat ttaaaactca cccccttatt aaatctgttc taacaggtgg
    15421 tgaacgaatt ggttatggag ccagagcact taatgaggga ggatggcaat ctctccctaa
    15481 acttaccttt cctggtggcg ccttgattgg agatgctgcc ggtttcctaa atgtgcctaa
    15541 aatcaaagga attcacaccg caatgcaatc tggaatgctt gctgcacaag cttgctttga
    15601 tgcgctaagt gaggaaaaga atggtcaatt tgagctaagc gcttactctg aaaaaatcaa
    15661 tcaatcctgg ctaaagcaag agctatatgc agtgcgaaat attcgaccgg gattcaaata
    15721 cggtttattc cccggtctaa ttaatgctgc cttggaaact tatgtaactc gagggtattc
    15781 tccatggact ttaaaaaatc atgcggacca taaatcgtta attccagcag aaaaagctaa
    15841 aaaaattaac tatcccaagc ccgatggcgt gctcacattt gataaactct catctgtatt
    15901 tttatcaaat acatttcatg aagaaaatca accctgccat ctcaagttaa aacaacccaa
    15961 actggccatt gaagtgaact taaaccaata cgcttcccca gaaagccgct attgcccggc
    16021 agctgtttat gaaataatcg agaatgttga aaaagggccc caattacaaa ttaatgccca
    16081 gaactgtatt cattgtaaaa cgtgcgatat taaagatccg cgtcaaaata tagtttggca
    16141 agcacccgaa ggtgggggtg gaccgaatta ctcaggaatg taaccaatca atcttaaata
    16201 atgtttttgg gtagagtcct gtaattatta gcagggatgt tgatataaaa catgaaaata
    16261 aggattattg catgtaccag ccttatcttt tatttgccat tttaattctt tctctcattc
    16321 tattcgtttg
//
LOCUS       DATNSN010000070        16078 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.070, whole genome shotgun sequence.
ACCESSION   DATNSN010000070 DATNSN010000000
VERSION     DATNSN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.070"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            101..2977
                     /gene="rapA"
                     /locus_tag="VAH76_001760"
     CDS             101..2977
                     /gene="rapA"
                     /locus_tag="VAH76_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEO1386377.1"
                     /translation="MTFRIGQRWISNTESQLGLGIITEVDGRQVSISFPAAGEDRIYA
                     IDNAPLSRIVYKTGEEILTNDQKRIKVTSIQEQDGVLLYTGNDDLGNVAQVSELALNC
                     FIKLNSPQQRLFSGLLDKLNAFKLRIKTLEHLGRLQQSKARGLLGSRTNHLIHQIYIA
                     YEVAQRFAPRVMLADEVGLGKTIEAGMILHYQLHTGRASRVLIVVPNSLIHQWFVEML
                     RRFNLHFSIIGPNQYDAMPSTLDELEDNDNLIEPASEDGNLFEGEQLVLTSLDFLMEN
                     ETARQQALAAQWDLLVVDEAHHLYWSEDSQSPEYTFIEKLSARGTGLLLLTATPEQVG
                     MKSHFARLRLLDPARFYDFSAFVNEEKQYHEVNKIVQDLMDYKEKNGVDELNPQLHSH
                     LKQLLGKEAPADINATIKELLDRFGTGRVLFRNTRAAIKGFPERRIHPVALTCPTLYS
                     EIIRESNLIHLYPETLLNNDSWIEHDPRVQWLVNKINELRPEKILVICAKAKTAISLE
                     QYLKLKTGIRSALFHEGLTIIERDRAAAYFAEQENGAQVLVCSEIGSEGRNFQFSHHL
                     ILFDLPLNPDLLEQRIGRLDRIGQNHPIEIHVPYLAGTAQEKLFRWYHEGINILQQSC
                     SVGFSIFETFENRLTPVLSNPMQETNEHSLEKLIADTQLHTQHIKEALNAGRDRLLEL
                     NSCNYTIGKELIEAIEAEENCLELENYMSQVYQEYGIDQEYHSENAEILRPGNHMKTA
                     HFPGLKEDGMTVTYSRPKALVREDMEFLSWEHPMVYDTMEMILESELGNATVTTISIK
                     SIKPGTLFLETFYTINSSAPKYLQLDRFIPSLPIRILMDTTGKNLSSILSYEQLNNLC
                     QPLKRHLGYPVIKQVREEIESILQQTNKIAEDQMKLFIGEAKSMMELTTSQEVNRLES
                     LQKINPAIRDEEIEFFKKKISESNYFINSSTLKLQAIRVVINVS"
     gene            complement(3091..4494)
                     /gene="ankH"
                     /locus_tag="VAH76_001761"
     CDS             complement(3091..4494)
                     /gene="ankH"
                     /locus_tag="VAH76_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkH/LegA3"
                     /protein_id="HEO1386378.1"
                     /translation="MSIANDIISHRMPDFDAYLRAGESLDDIDEYGFTPLIECAITRQ
                     IKIAEQLIARKVDINKPDVTGRTPLHWAVDNNDLDMTKLLLTYGADPNAYTRNGLCVL
                     VYPVLRGQDAIKQLLYHHGAKLDFALDFINAKLIGHRFELQGDVDIVNAKGEFIELDY
                     EGFILEFTVAVVKDSLRRFISSYSTRHLRDYFPYIHNIMDAFTDAAELLQYQHHPRLG
                     EQHFKRIAALLKAPMLILPAASRGHALCFVRYHQWWAKIDRGENSLQEGSVNIYRITR
                     PEALTINFIHDFLYKKQNRRYYHQMINQQLGLLPFAQMPISSQISGNCSWANVQAVVP
                     VAYSMQELTSVENFKPDVALSLYDEWVEWDKDRALDECIQRFYLASPERKASIAAILG
                     GILFQACDHANKRHLERAEKILNILILDEYYYVLSSYLEEYCIKRLTRKGNNLLKILD
                     DCGINPNIGVNPVATGL"
     gene            complement(4495..5268)
                     /locus_tag="VAH76_001762"
     CDS             complement(4495..5268)
                     /locus_tag="VAH76_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEO1386379.1"
                     /translation="MAKEYYLSDEIEEIVLASTVLSYSRPNLSSKRFNDLKNGKTPWE
                     AELDLNGLNVESARKSLYQFIQTQIKNNKQCVLIIHGADNSQDKPPLMKNLVNRWLPQ
                     INEVLAFHSAKPKDGGSAAVYVLLGSVFHVELPTRLPSNRSESTFLVAETKAMERRRR
                     LAEQQGERRFASVKAREHPEEEIKAAPEGELQNNILQHPELNSQRFDGIDPNLNPEPP
                     LNTEARREFDNERREQDKEKQLRLGNMPKFTTAPTPRGP"
     gene            complement(5417..6439)
                     /locus_tag="VAH76_001763"
     CDS             complement(5417..6439)
                     /locus_tag="VAH76_001763"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO1386380.1"
                     /translation="MSRHLNVAIVGATGAVGETFLTVLEERNFPIKSLYPLASSRSVG
                     KTVTFRDQELDVLDLAEFDFSKVDLALFSAGGAVSKEYAPKAVAAGCVVVDNTSCFRY
                     EDDIPLVVPEVNPHRIADYTKRGIIANPNCSTIQMVVALKPIYDAVGISRINVATYQS
                     VSGTGKKAISELVAQVGDLLNGRPANVQVYPQQIAFNALPHIDQFEDNGYTREEMKMV
                     WETRKIMEDDSIMVNPTAVRVPVIYGHSEAVHLELKKPLTADDARALLAKAPGVTVVD
                     NLSKASYPTAIKNAVGHDDVFVGRIRQDISHPCGLNLWIVADNIRKGAATNAVQIAEI
                     LQKEFL"
     gene            complement(6549..7607)
                     /gene="aroC"
                     /locus_tag="VAH76_001764"
     CDS             complement(6549..7607)
                     /gene="aroC"
                     /locus_tag="VAH76_001764"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO1386381.1"
                     /translation="MSGNTFGALFTVTTFGESHGPAIGCVVDGCPPGMSLTEADIQPF
                     LDKRKPGQSKYTTQRREEDKVQILSGVFDGKTTGAPIALLIQNTDQRSRDYEDIKNLF
                     RPGHADFTYHYKYGHRDYRGGGRSSARETAARVAAGAIARLYLKRYLNLDIIGYLQQM
                     GDLKLQFENENEINKNPFFCPNNKQIQELADYVDRLRRQGDSVGARVKILARGVPTGL
                     GDPVFDKLDATLAYAMMSINAVKGVEIGAGFNAVEQLGSHHRDQMTAKGFLSNHAGGI
                     LGGIATGQPIEVSIALKPTSSITTPGQTINTEGEEVTVVTKGRHDPCVGIRAVPIAEA
                     MMALVLMDHYLRHKAQCK"
     gene            complement(7619..8551)
                     /gene="prmB"
                     /locus_tag="VAH76_001765"
     CDS             complement(7619..8551)
                     /gene="prmB"
                     /locus_tag="VAH76_001765"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO1386382.1"
                     /translation="MVMVDTLVSSYFSKETEELVTILDFLRFSLSCAENAQLFYGHGT
                     DNAWDDMRSLILRSLSLPYDVDPFLLNARLTTSERKHLCNQLDKRINQRVPVPYLIKE
                     AYFCDLPFYVDERVLIPRSPIGELINNQFSPWIEAERVHHVLDLCTGSGCIAIACCYA
                     FPEAQVDAVDISNQALAVATINCERHDVGDQLALIESDCFTALSGKQYDLIVSNPPYV
                     GKEEMQTLPDEYRHEPVLALETGNNGLAIIEKILKNAHAYLSEHGILVVEVGNSEEAL
                     CETYPMVPFTWLEMSNGGQGVFLLTKQQLEDYFK"
     gene            8716..9285
                     /locus_tag="VAH76_001766"
     CDS             8716..9285
                     /locus_tag="VAH76_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEO1386383.1"
                     /translation="MTDDFLSDEDKALFRHHMRSVKPLNEKTKRVKSPTQPTHEKNTT
                     TFKSQPVPKEEYFLSDFIRDTVQPDTILSFSEPGIPNQRFRALKNGQIPWEAKLDLHG
                     LKAEAARQALYDFIQTQSLNNKRCLLIIHGKGGHQGAPPVIKNLINRWLPQFSQVLAF
                     HSALPKDGGLGAVYVLLKRNREKDEQGNY"
     gene            9358..9777
                     /locus_tag="VAH76_001767"
     CDS             9358..9777
                     /locus_tag="VAH76_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO1386384.1"
                     /translation="MEKLGQFIINHWQLWLAFVVVVFLIFINEILSQRKKAKEVSPQS
                     LVDLMNNGNAIVIDIRDKESFKNGHIINSMNVNPDDFDQQKMSKYKDKPIILVCARGL
                     QSPALAMKIRNQGYQPLVLSGGIQAWQNADLPLVKGK"
     gene            9782..10036
                     /gene="grxC"
                     /locus_tag="VAH76_001768"
     CDS             9782..10036
                     /gene="grxC"
                     /locus_tag="VAH76_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEO1386385.1"
                     /translation="MNEVILYTTGYCPYCIKAKELLDRKKVIYTEIRVDLQPELREEM
                     IQKSGRRTVPQIFINGQAIGGCDDLYALEAQGTLNELLKK"
     gene            10046..10534
                     /gene="secB"
                     /locus_tag="VAH76_001769"
     CDS             10046..10534
                     /gene="secB"
                     /locus_tag="VAH76_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEO1386386.1"
                     /translation="MTEQLNTNQQNDEAQFMIQRIYIKDLSYETPNTPAVFQQQWEPE
                     LKLDLNTTTTQLDKNVYEVVLTVTTTVMNQKTTAFLVEVKQAGIFTIQGAAASQLDHL
                     LHSFCPSILFPYAREAITSQVIRGSFPQLVLAPINFDALYMQQLEEKQKATGAKDETV
                     SH"
     gene            10536..11525
                     /locus_tag="VAH76_001770"
     CDS             10536..11525
                     /locus_tag="VAH76_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO1386387.1"
                     /translation="MNKKTIAMLGAGSWGTAVAIHLAKIGHKTLLWSHNPQHVALMAE
                     QHSNPAYLPGIPFPENLIPSDDLIECVQLADYVIIAVPSHAFAEIINKIPKPTQGLAW
                     LTKGVDPASHQLLSQLVASRFGVDFPIAVISGPSFAKEVARFLPTALTLASNNTNYQK
                     KMHQLFHHDNIRVYLSDDLIGVQLCGAVKNILAIACGISDGLGYGANAKAALITRGLA
                     EMTRLGLSMGARQDTFLGLAGVGDLVLTCTDDQSRNRRFGLLLGREVPIPEAEHQIGQ
                     VVEGKHNAAQICAIANKNKVEMPICEQINALLHGIVHAQQAVNNLMSRPAKEE"
     gene            complement(11611..12450)
                     /locus_tag="VAH76_001771"
     CDS             complement(11611..12450)
                     /locus_tag="VAH76_001771"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO1386388.1"
                     /translation="MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYL
                     GDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIP
                     VLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSSRACSVLVA
                     LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKGVAIVDSAQ
                     ATAISLYELLQNQNLYNDNLEVLPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELID
                     AMG"
     gene            complement(12522..15989)
                     /locus_tag="VAH76_001772"
     CDS             complement(12522..15989)
                     /locus_tag="VAH76_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interaptin"
                     /protein_id="HEO1386389.1"
                     /translation="MILEEYIRMAKNKEFFDALGEIAKSAKSDETLRDELAKVLDDIL
                     KTDPSDPDAFRKIIVEHQEFWDEHNPSLMKFEEGWFLGPSTEEYLESDDFLDSNDPIN
                     SFQKLHQFAAEQRVKLGLEKSDTDTLVAILKNNPEECRAYIESKKPGLGNFSEGNVHG
                     WLKEEYTPTIPPKAINKSTGVLSDGAIKKIKEQASELLLLKLIKNCENRQLLKDLLTA
                     NSKTEAEHAANAIGFPTEGNGELSYPLSDEVEEALDDIIQELEEEAAKAGFDSYVQSL
                     SHDALLAKKNGLESTTAAGFKNSLDEPYKTYLPDSEWETAQRVLGARYLQAVLSSGAQ
                     NLKDALNAKDANALIAELKKPALLGPHDYIDKAVTEENLGSLKKSMMKSFINNIKDEP
                     NLKALDALKALDGAKNLDKFKEVLGKLGITPADWVKDTDLKDMKQWARARQFELEINR
                     VSSLGSGAHSKLMSTLTQLPVEKQREILAKPQQLRHLMNAYEPHVAEHYLGKNASGIA
                     ELLTENKRLEGFRAIHNAEVARVLANFKPEITLSDKQVAAINQALNSANSNPNTYTQV
                     ADYKTLIDAIKIQSGPVNQKDFYNAFNLNDDGSDFTRPTPRKDEMSKQQQYNQHIYAE
                     YNSTSNSGNKKLLAVLLSIEKPTTFSKDIVNQFLRPLKDRKTPQEYADRLFGENPPNP
                     TNKKFKDDLLRELTPTLFNEIKNDLRKQELLDTNPTHVMIAINALSTELESVKGITGP
                     IRTNADKLKFINDIDPVHLYNPTFQGTARSKAAQMKARYQGLSRDCELVVDQLRRQVI
                     ALEGHLKSLPQDGQFKATGLTLEQKEEIKKLRAALQTELDAVRKDLDFYKKIQGKLET
                     IIKEVDVAAKGKMQYYYNSEGIKRHPPVSPDQIPPLPNVPNPSLNSNTTVTTGSSGKI
                     QEFLIGEKIPEGQVAVVDVSHKTTSKSGAPIETVGRYTQTKHEGGSKFEIVQFPTQVP
                     PPARPSDDPLVEAKVNFVMAKAADILASLDAPPTKDRPIRLRGSNPEELEYLYTALVI
                     LGEKNPKMKFSRDAIEVNSAVFHPDNVKGRLWGFSSNSLYSQVFTNTGLTEAQNIIQS
                     KIKHMQQMTDEKFSPQKEREKVDSKVQEITDKQSKMKKELNPVHKTTERTIEQEGPAP
                     ESPSTGMRK"
BASE COUNT         4796 a         3500 c         2964 g         4818 t
ORIGIN      
        1 ccactaaaat tatttctgta acttgtatca aaaattgata caattacgca ttaaaacata
       61 cagttcattt tcgtttttaa taatgaggac gtttctctaa atgacatttc gcattggtca
      121 acgctggata agtaataccg aatcacaact gggtttaggc attattactg aagtagatgg
      181 cagacaagtt tctatcagtt tccctgctgc tggggaggat agaatttatg ctatagataa
      241 tgcaccatta agccgcatag tctataaaac aggtgaagaa atacttacca atgatcagaa
      301 aagaataaaa gtaacttcca tccaggaaca agatggtgtt ttactgtata caggaaatga
      361 cgacttggga aatgtggctc aagttagtga gttggcactt aattgtttta tcaaacttaa
      421 ctcccctcaa caaaggctct tcagcggatt attggacaaa ctaaatgctt ttaaactcag
      481 aattaaaaca ttagagcatt taggccgatt acaacagtct aaagcaagag gcctattagg
      541 ttcacgaact aaccatctta tccaccaaat ctatatcgct tatgaagttg ctcaaagatt
      601 tgctcccaga gtgatgcttg ccgacgaggt tggattgggg aaaaccattg aagcgggaat
      661 gattctgcat tatcaactgc atactggccg tgcttcacgc gtactgatcg ttgttccaaa
      721 ttcattgatt caccaatggt ttgtcgaaat gttaaggagg ttcaatctgc atttctctat
      781 cattggtcca aatcaatacg atgccatgcc atccacactt gatgagttgg aagataacga
      841 caatctaatc gagccagcat cagaagatgg caatctgttt gaaggtgaac aactggtatt
      901 aacaagcctg gatttcctta tggaaaatga aacagcacgt caacaggcat tagctgcgca
      961 atgggatctt ctggtagtag atgaggcgca tcatctttat tggtcggaag actcacaaag
     1021 tcccgaatac acgttcattg aaaaattatc cgctcgaggc acaggcctgc ttcttcttac
     1081 cgcgactcct gaacaagttg gaatgaagag tcatttcgct cgtcttcgtc tacttgatcc
     1141 agcacgcttt tatgattttt cagcctttgt aaatgaagaa aagcaatacc atgaagttaa
     1201 taaaatagtt caggatctca tggattataa ggaaaaaaat ggcgtagatg agttaaatcc
     1261 ccaactccac tctcatttaa aacaactctt gggtaaagaa gctcctgctg acattaatgc
     1321 aacgatcaaa gagttgcttg atagattcgg cacaggtcga gtactttttc gcaatactcg
     1381 tgcagcgatt aaaggatttc ccgaaagaag aatacaccct gtcgcattga catgcccaac
     1441 gctttattcc gagataatca gagaatccaa cttaatccat ctttacccag aaactttgct
     1501 taataatgac tcctggatag agcatgaccc ccgagtgcaa tggcttgtta acaaaattaa
     1561 tgaattacgt ccggaaaaaa ttctggtaat ctgcgcaaag gcaaaaacag ccattagtct
     1621 ggaacagtat ttaaaattaa aaacaggcat tcgaagtgct ttatttcatg aaggacttac
     1681 cattatagag cgtgacagag ctgccgctta ttttgcagaa caagaaaatg gtgcacaggt
     1741 tcttgtttgt tccgagatag gaagtgaggg aagaaacttc cagttttccc atcatttgat
     1801 tttgtttgat ttgccattaa accctgacct tttagaacag cgtatcgggc gtttggatcg
     1861 tattggtcaa aatcatccca ttgaaattca tgtaccctat ttagcgggta ctgcgcagga
     1921 aaagctattt cgctggtatc acgaaggaat caacattctt caacaaagct gctctgttgg
     1981 cttttcaatt tttgaaactt ttgaaaatcg gctgacacct gttttatcta atcccatgca
     2041 ggagacgaac gaacactcat tagagaagct tattgctgat actcaattgc atacccagca
     2101 tattaaggaa gcccttaatg caggaagaga tagattacta gaattaaact cctgtaatta
     2161 cactataggg aaagaactca ttgaagcgat agaagcagaa gaaaattgcc ttgaacttga
     2221 aaattacatg tcgcaagtgt accaggaata tggaattgat caggaatatc attcggagaa
     2281 tgccgaaata ttgcgccctg gtaaccacat gaaaaccgct cattttcctg gactaaaaga
     2341 agacggtatg accgtcactt attctcgccc taaagccctt gttcgggaag acatggagtt
     2401 tttaagttgg gaacacccca tggtttacga cactatggaa atgatcctgg agtcagaact
     2461 tggcaacgcc acagtgacta ctatttcaat caaaagcata aaaccgggta ccctcttcct
     2521 ggaaactttt tataccatta attcttccgc ccctaaatac ttacaacttg accgatttat
     2581 tccttcattg ccaattcgaa ttttgatgga tactaccggt aaaaatttat caagcatttt
     2641 aagttatgag cagcttaata atctgtgcca accacttaaa cgtcatctgg gttaccctgt
     2701 tattaaacaa gttcgtgaag agattgaatc cattctgcaa caaaccaaca aaatcgcaga
     2761 agatcagatg aaattattta ttggagaagc aaaatcaatg atggagctaa cgacttctca
     2821 agaagttaat cgactggaat ccttgcaaaa aattaatccc gccattcgtg atgaagaaat
     2881 tgagttcttt aaaaagaaaa tttcagagag taattacttc attaatagct ccactttaaa
     2941 acttcaagcg attcgggttg taatcaatgt cagttagtta atcatccctt gacaaattga
     3001 cacgagaata aaagaaattg cttgtcttac aggttagacg ctgatttcac aaaatcaaca
     3061 actattagat tattaaagga aaatttctgt ttataggcct gtcgcaacag gattgacgcc
     3121 aatattagga ttaatcccac aatcatccag aattttcaat aaattatttc ctttacgggt
     3181 taatcgttta atacagtatt cttccagata actgctcaaa acataataat attcgtctaa
     3241 aatcagaata ttaagaattt tctccgctct ttccagatga cgcttgttag cgtgatcaca
     3301 agcctgaaac aaaattcctc ccaagattgc agcaatactt gccttacgtt caggactggc
     3361 taaataaaat ctttgtatgc attcatcaag agccctgtct ttatcccact cgacccactc
     3421 atcatacagt gataaggcca catcaggttt aaaattttct acgctggtca gctcttgcat
     3481 agaatacgcg actggtacaa cggcttgcac attagcccac gaacaatttc cactaatttg
     3541 agaactaatt ggcatttgag caaaaggaag cagccccaat tgttgattaa tcatttgatg
     3601 ataatatcgt ctgttttgtt ttttatataa aaaatcatgg ataaaattga ttgtcagtgc
     3661 ctctggcctg gttattcggt aaatattaac acttccttct tgtaagctgt tttcacccct
     3721 gtcaattttt gcccaccact ggtgataacg gacaaagcat aaggcatggc cacgactcgc
     3781 cgcaggaaga atcagcatag gggcttttaa caaagcagcg atacgtttga aatgctgttc
     3841 gccaagtctg ggatgatgtt gatactgcaa cagttctgcc gcatcagtaa aagcatccat
     3901 aatattatga atatatggaa aataatccct taaatgccga gtcgaataac tactgataaa
     3961 tctccgcaag gagtctttta caacagcaac agtgaattcc aaaataaacc cttcataatc
     4021 aagttcgatg aattcacctt tcgcattgac aatatccaca tcaccttgca attcaaatcg
     4081 atgaccaatc aattttgcat taataaaatc aagagcaaaa tcaagcttcg caccatgatg
     4141 atataacaac tgctttattg catcttggcc acgcaaaacc ggataaacca acacacaaag
     4201 accattacga gtgtaggcat taggatcagc gccgtaggtt aataagagtt tggtcatatc
     4261 caaatcatta ttatccactg cccaatgtaa tggagtgcgt cctgtgacgt ctggcttgtt
     4321 gatatccact tttcgagcaa ttaactgctc tgctatttta atttgtcgtg taatggcaca
     4381 ctctatgagg ggagtaaaac catattcatc gatatcatcc aaagattccc ctgcacgcag
     4441 ataagcatca aaatcaggca ttctatgact gataatatcg tttgcaatac tcatttaggg
     4501 cccccggggg gtaggtgctg tagtaaattt tggcatattg cctaagcgta attgtttttc
     4561 tttatcctgt tctcttctct cattatcaaa ctcccttctt gcttctgtat taagcggcgg
     4621 ttcagggttt aaattaggat ctattccatc aaaacgctga ctattcaatt cagggtgttg
     4681 caatatattg ttttgcaact ccccctctgg agcagccttg atttcttctt cagggtgttc
     4741 acgcgcttta acagaagcaa accttctctc tccctgctgt tcagccaaac gtcttcgtct
     4801 ttccattgct tttgtttcag caaccaagaa agtactttca ctgcgattgg aaggcaatcg
     4861 tgtgggtaac tcaacatgaa atactgagcc caacaagaca taaacagctg cagaaccacc
     4921 atccttgggt ttggcgctat gaaatgccaa tacctcattg atctgaggca accaccgatt
     4981 caccagattt ttcattaaag gcggtttgtc ctgagaatta tccgcgccat gaataattag
     5041 aacgcattgt ttgttatttt ttatttgagt ttggataaat tgatataagc tcttacgagc
     5101 agattcaacg tttaaaccgt ttaaatccag ttccgcttcc cagggagttt ttccattttt
     5161 taaatcattg aaacgttttg atgataaatt gggtcgagag taagacaata cagtactcgc
     5221 gagcactatt tcttcgatct catcggataa ataatattct ttcgccatct ttttcaaaat
     5281 ttaacagtca taatagaagt ataacttagg atataaaaag tatggcattt tttctaatat
     5341 aacctgcatg accttatgga ggcatacagg ttttctatca agatactgga cctatgctat
     5401 tgcgggagcg ggagcttcac agaaactctt tttgcagtat ttcagcaatt tgtaccgcgt
     5461 tagttgccgc acctttgcga atgttatccg cgactatcca caaatttagt ccacaaggat
     5521 gagaaatatc ctgccttatg cgccctacaa aaacatcatc atgcccgact gcattcttaa
     5581 ttgctgtggg ataacttgct ttagaaagat tatccactac agtaacgccg ggtgcctttg
     5641 ccaaaagcgc gcgagcatcg tcagccgtca aaggcttttt taactccaga tgaaccgctt
     5701 cagaatgccc ataaataaca ggaaccctga cggctgtagg gttaaccatg atgctgtcat
     5761 cttccataat cttgcgggtt tcccagacca tcttcatctc ttctcgggta taaccattgt
     5821 cttcgaactg atcaatatga ggaagcgcat taaaagcaat ttgctgagga tagacttgaa
     5881 cattagctgg tctcccattt aaaagatcgc ctacctgagc aaccagctcg ctgatggctt
     5941 ttttgccagt cccggaaaca gactggtaag tcgccacatt aatacgacta ataccaacag
     6001 cgtcatagat tggctttaga gcaacgacca tctgaatggt agagcaatta ggattagcaa
     6061 taatgcctct tttagtataa tcagcgattc gatgaggatt cacttcaggg acaacaagag
     6121 gaatatcatc ctcataacga aaacaggagg tattatcaac caccacgcat cccgcagcca
     6181 cagcttttgg cgcatactct tttgaaacag cgccgcctgc agagaataaa gcaagatcaa
     6241 ctttgctaaa atcaaattct gctaaatcca aaacatccaa ttcctgatct ctaaaagtca
     6301 ctgtcttacc tactgagcga gaactcgcta atgggtaaag ggacttaatt ggaaagtttc
     6361 tttcttccaa aacggttaaa aaggtttctc caacagcgcc ggtggcacct acgatagcta
     6421 cattcaaatg tctgctcatt atgacctcat cttaccatca attaacaatg tgctagtcaa
     6481 gtacaattcc tgcttgcgca gcagaaaacg gactacgata tggctatatt ttgaaacgtt
     6541 ataatttatt atttacactg cgctttatgt cgcaaataat gatccattaa caccaaagcc
     6601 atcattgcct ctgcaattgg cacagccctt atccccacgc agggatcatg tcgccctttg
     6661 gttactacag taacctcttc accttcagta ttaatagtct ggcctggagt tgtgatgctt
     6721 gaagttggct ttaaagcgat gctcacctcg atgggttgcc ctgtcgctat gcctcctaaa
     6781 ataccaccgg catgattact caagaatccc ttggcagtca tctgatctct atgatgactt
     6841 cctaactgct ccactgcatt aaaaccagct ccaatttcaa cacctttaac agcattaatt
     6901 gacatcattg cataagcaag agtagcgtcc agtttatcaa atacagggtc tcccaatcct
     6961 gttggcactc cccgtgccag tatctttacc cgagcaccaa cggaatcacc ctggcgtctt
     7021 aaacgatcta cataatcagc cagctcctga atttgcttat tattaggaca aaaaaagggg
     7081 tttttattga tttcattttc attttcaaac tgtaacttca aatcgcccat ttgttgtaaa
     7141 taaccaataa tatccaagtt taaataacgc tttaaataca atctggcaat agcaccggca
     7201 gcgactcgtg cggctgtctc acgagcggaa gaacggcctc cccctctata atctctatgc
     7261 ccatatttat aatgataggt aaaatcagca tgtcctggac ggaacaaatt tttaatgtct
     7321 tcataatccc gggaacgctg atcagtattc tgtatcagca atgcaatggg agctcctgtc
     7381 gttttcccat caaatacacc agaaagaatc tgtaccttat cttcctctcg tctctgggta
     7441 gtatatttgg attgacccgg tttacgtttg tctaaaaaag gttggatatc tgcttcagtt
     7501 aaagacatgc cgggaggaca accatcgact acacatccga tagcaggacc gtggctttca
     7561 ccaaaggttg ttacagtaaa taacgctcca aaggtattcc ctgacatgcc ttactcgctt
     7621 atttaaaata gtcttctaat tgctgttttg ttaataaaaa caccccttgc ccgccgttgc
     7681 tcatttcaag ccaggtaaaa ggaaccatag gataagtctc gcataaagcc tcttcactat
     7741 tacctacttc cactaccaaa atcccatgtt cacttaaata agcatgggca ttctttaaaa
     7801 ttttttcaat aatagccaag ccattatttc ctgtttccaa tgccaaaacc ggttcatggc
     7861 ggtattcatc cggcagggtc tgcatctctt ccttccctac ataaggagga ttgctcacga
     7921 tcaaatcata ttgtttacct gacagtgccg taaaacaatc cgattcaatc agtgccaatt
     7981 gatcgcctac atcatgacgt tcacaattga tagtagcgac agccaatgct tgatttgaaa
     8041 tatccacagc atccacctga gcctcaggga atgcatagca acaggcaatc gctatacaac
     8101 cactcccggt acataaatcc aaaacatgat gcactcgttc tgcttctatc caaggggaaa
     8161 attgattatt gattaactct cctattggtg atctgggtat aagcactcgt tcgtcaacat
     8221 aaaaaggcaa atcgcaaaaa taggcttcct taattaaata aggtacgggt actctttgat
     8281 taattcgttt atccaattga ttacaaagat gtttgcgttc actggttgtt aaacgtgcat
     8341 tcaacaaaaa agggtcaaca tcgtatggta aagacaagct tctcaatatc agggatctca
     8401 tgtcatccca agcgttgtca gttccgtgac cataaaataa ttgcgcgttt tctgcacaag
     8461 acaaactaaa ccgtaaaaaa tccagaatag taaccagttc ttctgtttcc ttgctaaaat
     8521 aactactcac aagcgtatca accataacca tagaaaaagt acaaattgta cactaaatta
     8581 aaatctatgt cctgtattct ttgttaaggg gtgcgattta agacgcccct tcgcatggct
     8641 cgattttcac ctaacctaaa gaattacttc aaattaagtt ataattaaat atattctttt
     8701 gatgcctgtt aaaaaatgac tgatgatttc ctgtctgatg aggataaagc tctatttcgt
     8761 catcatatgc gttctgtaaa accattaaac gaaaaaacaa aacgcgtaaa aagccccacg
     8821 caacccactc atgaaaaaaa taccacaact ttcaagagtc agccggtacc caaagaagaa
     8881 tattttttat ctgattttat tcgtgacact gtccaacccg atactatttt atctttttca
     8941 gaacctggca ttcccaatca gagattccgt gcactaaaaa atggacaaat tccatgggag
     9001 gccaagctgg atttacatgg attaaaagct gaagcagctc gccaagcgct ttatgatttt
     9061 atccagacgc agtcactcaa taataaacgc tgcttactca ttattcacgg caaaggaggc
     9121 catcagggcg ctcccccggt aataaaaaac ctaatcaatc gctggctgcc gcaatttagt
     9181 caagtcctgg cattccacag cgctcttcca aaggatggag gattgggagc tgtgtacgtc
     9241 ttattaaaaa gaaacagaga aaaggatgaa cagggcaatt actagtaaca ctttacttgc
     9301 gttaaagcag taatcttagg ataattcgtc aaattaaaaa atatcttgag tacagctatg
     9361 gaaaaattag gtcagttcat catcaatcat tggcaactct ggctcgcctt tgttgtggtt
     9421 gtatttttaa ttttcattaa tgaaatactc tctcaaagaa agaaagccaa agaagtttca
     9481 ccgcaatccc ttgttgattt aatgaacaat ggaaatgcca tcgtaatcga catacgcgac
     9541 aaagaatcat tcaaaaatgg ccatatcatt aattcaatga acgtaaaccc agatgatttt
     9601 gatcaacaaa aaatgagcaa gtacaaagat aaacccatta ttctggtctg cgcacgaggc
     9661 ttacaatctc cagccctagc catgaaaatc cgcaatcaag gttaccaacc cctggttttg
     9721 agtggaggaa ttcaagcctg gcaaaatgct gatcttcctt tagtgaaggg taaatgaaca
     9781 gatgaatgaa gtcatactct acactacagg ctattgtcct tattgcatca aagcaaaaga
     9841 actactagat agaaaaaagg ttatctatac cgaaatccgc gtcgatttac agcctgaatt
     9901 acgtgaagaa atgatacaaa aaagtggccg gcgtacagtg cctcaaattt tcattaatgg
     9961 ccaggcaatt ggtggctgcg atgatttata tgccctggaa gcccagggaa cattaaatga
    10021 acttttaaaa aaataggaat aaaacatgac tgaacaactg aataccaatc agcaaaatga
    10081 tgaagctcaa ttcatgattc aacgaatcta tatcaaagat ttatcctatg aaacaccaaa
    10141 tacaccggct gtttttcaac aacaatggga accggaactc aaactggatc tcaatacaac
    10201 gactactcaa ctggataaaa acgtttatga agtggtttta actgttacaa ccactgtgat
    10261 gaatcaaaaa accacagcgt ttttggttga agtaaagcaa gcgggcatct ttactatcca
    10321 aggcgccgca gcaagtcagt tggatcactt gcttcatagc ttctgcccca gcatcctgtt
    10381 cccctatgct cgtgaagcaa ttacctcgca agtcattcgt ggcagcttcc cccaattagt
    10441 actcgcaccg attaattttg atgctttata tatgcagcag ctggaagaaa aacaaaaagc
    10501 aactggagct aaagacgaga ctgtttctca ctaagatgaa taaaaaaacc attgccatgt
    10561 taggtgccgg ctcctggggc actgctgtcg caattcattt agctaaaata gggcataaaa
    10621 cgctgctgtg gagccataat ccacagcatg ttgccctcat ggctgaacag cattcaaatc
    10681 cagcttacct tcctggtata ccttttccag agaatttgat tccttctgat gatttaattg
    10741 aatgcgttca attagcagat tatgtcatca tagcagtccc ttctcatgcc tttgcggaaa
    10801 taatcaataa aatccctaaa ccaacccaag gcttggcgtg gcttacgaag ggtgttgatc
    10861 cagcgagtca tcaattatta agccaactgg ttgccagtcg atttggggtt gattttccca
    10921 tagcagttat ttcaggccca tcctttgcca aagaagtggc tcgcttcctg cctacagctc
    10981 tcactttggc cagtaacaat acgaactatc aaaaaaaaat gcatcaacta tttcatcacg
    11041 ataacattcg tgtctattta agtgatgatt taataggtgt gcaattatgt ggcgctgtta
    11101 aaaatatcct ggcgatagcc tgtggaatca gtgatggctt aggctatggc gctaatgcca
    11161 aggcagcgct tattacccgt ggacttgctg aaatgactcg tttgggatta agcatgggag
    11221 cgcggcaaga tacttttcta ggcttggctg gtgtagggga cttggtattg acttgtacag
    11281 atgatcaatc gcgcaatcgc cgctttggat tgctcttggg tcgtgaagtt cctattcctg
    11341 aagccgaaca ccaaattggt caagtagttg aagggaaaca caatgcagca caaatttgcg
    11401 ctatagccaa taaaaataaa gtggaaatgc caatttgtga gcaaattaat gcgctattgc
    11461 atggtattgt tcatgcccaa caggccgtga acaacttgat gagtcgtccc gccaaggaag
    11521 aataaccttt gcccttgtaa agaatcatat taaattaggt ttagtgcacc aggacaccag
    11581 gacgcaggtg atactggtgg attgtcgaca ttaacccata gcatcaatca attcaatatc
    11641 actcaaactc aattgctcgc ctaaaaatat ctcacctacc ttctggaacc tattgataga
    11701 atctgttacc aaatagttga ttctggggag aacttccaaa ttgtcattgt acagattttg
    11761 attttgcaat aattcataaa gagaaattgc agtcgcctga gcagaatcga caattgccac
    11821 accttttggc aataagctgg ataacaaggg cttaaatact ggaaaatgag tgcaccccaa
    11881 taacaccgca tcttcatccg aaaaatcgtc gagataatgc ttcaatgcct cacgagcaac
    11941 tgcattagtc accatacctt cttctgccaa agcgactaaa acgctacatg cccgactgct
    12001 gacaacggcc tcaggcaaat tctttatgat caaatcatga taggctctgg aagcaatggt
    12061 tgtttctgta gccagcacag caatacgatg atttttggta gctgacaccg cagcggctgc
    12121 accaggtgcc accacaccca gaaccggaat atcagttaac atgctctgta aatggggcaa
    12181 ggcagccgtc gttgcggtat tacaggcaat gactaaggct tttatgcgcc gctcaaccag
    12241 aatttttgcc atttgcatag cgtattgttt gacagtatcg ggacttttcg taccataagg
    12301 taagcgggct gtatctccca aataaacaaa agattcatga ggtaatttat cccgcagagc
    12361 ccttaaaacc gtaagccccc ccatgcctga atcgaataca ccaatagcca aatccttgtt
    12421 agagttcaat tgattactcc ttcttgtcat ttatttcttg gctctcttat taggcaaaat
    12481 agcttcgttc taccaaaaat gagagccaaa gtcattatta tttacttacg catccctgta
    12541 ctcggacttt caggtgctgg tccttcttgt tcaatggttc tttcagttgt tttatgcacc
    12601 ggattcagtt ctttcttcat cttgctttgt ttatcagtaa tctcttgtac tttggaatcc
    12661 actttttctc tttctttctg aggactaaat ttctcatcag tcatttgttg catatgtttt
    12721 attttagact gtatgatatt ctgggcctct gttaatcctg tgttagtaaa tacctgacta
    12781 tataatgaat tgctgctaaa tccccacaac cgtcctttca cattatccgg atggaatact
    12841 gcagaattca cctcaatcgc gtcacggcta aatttcattt tgggattctt ttcaccaaga
    12901 attaccaagg cagtgtacaa gtattccagc tcttcaggat tcgatccacg taatctgatt
    12961 ggtctatcct tcgttggagg tgcatccaga cttgctaaaa tatcagctgc cttagccata
    13021 acgaaattca ctttagcttc taccaaagga tcatcgcttg gacgggccgg agggggaact
    13081 tgtgtcggaa actgtacaat ttcaaattta cttccccctt cgtgtttggt ctgagtatac
    13141 cgaccaaccg tttcaatggg agcacctgat ttcgatgttg ttttatgaga tacatcaacg
    13201 acagcaacct gtccttccgg tattttttca ccaataagaa attcttgtat ctttcccgaa
    13261 gatcccgttg taacggttgt attcgagttc aatgaagggt tgggaacgtt tggtaatggg
    13321 ggtatctgat caggtgaaac aggaggatgg cgtttgatac cctcagaatt gtagtagtac
    13381 tgcatcttcc cttttgctgc cacatccact tcttttataa tggtttctaa ctttccctgt
    13441 attttcttat aaaaatccaa atccttgcgc accgcatcca attcggtttg caaagcggca
    13501 cgcaattttt tgatttcctc tttttgttcc agtgtcaaac ctgtggcctt aaattgacca
    13561 tcttgaggca agctcttcaa atggccttcg agagcaatca cctgacgtcg taattgatca
    13621 acaaccagct cacaatccct ggataatcct tggtaccttg ccttcatctg agccgctttc
    13681 gatctggcgg taccctgaaa ggttgggttg tataaatgaa ccggatcaat atcattaata
    13741 aactttaatt tatctgcgtt tgtccttatt ggtcctgtga ttcctttaac agactctaat
    13801 tcagtactta aagcattgat tgctatcatc acatgagtag gattagtatc taacagctcc
    13861 tgcttcctga gatcattttt tatctcatta aataaagttg gtgtaagttc ccgcaacaaa
    13921 tcgtctttaa attttttatt tgtcggattg ggtggatttt ctccaaataa tctatcagca
    13981 tactcttgag gagtttttct atccttcaga ggacgtaaaa actgattgac tatatctttg
    14041 gaaaaggtgg taggtttctc tatgctcaac agaacagcta ataatttttt attacctgaa
    14101 ttagaagtac tgttgtattc agcatatata tgttggttat attgttgctg cttactcatc
    14161 tcatcttttc gaggagttgg tctggtaaaa tcagagccat catcgtttaa attaaacgcg
    14221 ttataaaaat ctttttgatt taccgggcca gattgaattt taatggcatc aatcaaggtt
    14281 ttatagtcgg caacctgagt ataggtattg ggattggaat tggcgctgtt taatgcctga
    14341 ttaatagcag cgacttgctt atcacttaaa gtgatttcag gtttaaaatt agccaaaacc
    14401 ctggctacct cggcattgtg aatggcccta aatccctcga gtcttttatt ttctgtcaat
    14461 agctctgcaa tcccggatgc attttttcca aggtaatgtt ctgctacatg aggttcataa
    14521 gcattcatta aatggcgtaa ttgttgtggc ttggccaaaa tctctctttg tttttcgact
    14581 ggaagctggg ttaatgtgct cattaatttc gaatgggccc cgctgcctaa agaagacact
    14641 ctatttattt caagttcaaa ctggcgagct cttgcccatt gcttcatatc tttcaaatcg
    14701 gtgtctttca cccaatccgc tggagtaata cctaacttcc cgagcacctc tttaaattta
    14761 tccagatttt tagccccatc cagtgccttt aaagcatcca gtgcctttaa atttggctca
    14821 tccttaatat tattaatgaa gcttttcatc atgctttttt tgagcgagcc taaattttct
    14881 tctgtcaccg ctttatcaat gtagtcatga ggtcccaaaa gggcaggttt ttttaattca
    14941 gcaattaatg cattggcatc tttagcattg agagcatcct tgagattttg ggctcctgat
    15001 gataaaaccg cttgaagata acgagctccc aatactcttt gggctgtctc ccattccgag
    15061 tccggtaagt acgttttgta aggttcgtcc aaactgtttt taaaccctgc cgctgtcgta
    15121 ctttctaacc cgtttttttt ggctaatagc gcatcgtgag ataatgattg gacataacta
    15181 tcaaatcctg ccttggcagc ttcttcttcc agctcctgaa ttatgtcatc caaagcttct
    15241 tctacttcat cacttaaggg ataagataat tctccatttc cctcggttgg aaaacctatg
    15301 gcatttgcgg catgctctgc ttcagtttta ctattagctg ttagcaaatc tttcaataat
    15361 tgccggtttt cacagttttt aatcagttta agcaataata attcgcttgc ctgttcttta
    15421 atctttttga ttgccccatc agataaaaca ccagtagatt tgttaatagc tttcggggga
    15481 atcgtcgggg tatattcttc ttttaaccac ccatgcacat tcccttcact aaaattaccc
    15541 aaaccaggtt ttttggattc gatatacgct ctgcactctt cagggttgtt ttttaaaata
    15601 gcaaccaaag tatcagtatc tgatttttcc aagccaagct tgactcgttg ttcagcagcg
    15661 aattgatgta atttttgaaa actgtttatt gggtcattag aatcgaggaa atcatctgat
    15721 tctaaatatt cttctgtgct tggtcccaaa aaccaccctt cttcaaactt catcaagctg
    15781 ggattatgct catcccagaa ttcctgatgc tcaactatta tctttctaaa agcatcagga
    15841 tcagaaggat cagttttcag tatatcatct aaaacttttg caagctcatc acgaagagtt
    15901 tcatcacttt ttgctgattt cgcaatttct cccagcgcat caaaaaattc tttatttttg
    15961 gccatacgga tgtactcctc gagaatcatt atgtgttcat tttaagccaa aaaacttaag
    16021 ttatccttaa attttgccga tattaatacc acagatgaaa acctaagcta gctttata
//
LOCUS       DATNSN010000071        15901 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.071, whole genome shotgun sequence.
ACCESSION   DATNSN010000071 DATNSN010000000
VERSION     DATNSN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.071"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..303
                     /gene="tuf"
                     /locus_tag="VAH76_001773"
     CDS             <1..303
                     /gene="tuf"
                     /locus_tag="VAH76_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO1386390.1"
                     /translation="KPGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDV
                     TGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE"
     gene            365..440
                     /locus_tag="VAH76_001774"
     tRNA            365..440
                     /locus_tag="VAH76_001774"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:398..400,aa:Trp,seq:cca)
     gene            454..825
                     /gene="secE"
                     /locus_tag="VAH76_001775"
     CDS             454..825
                     /gene="secE"
                     /locus_tag="VAH76_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO1386391.1"
                     /translation="MKSSNENQGKMKDIMSWLAIILATAGAFVCTYYYTFTGPIQSII
                     WLAWLILILFLGYLTTTGKRVFVFAQEAKVELLKVVWPTRQETIQTTTIVMVMVGLTG
                     FILWGVDSIMMWAIAKLTHLG"
     gene            828..1376
                     /gene="nusG"
                     /locus_tag="VAH76_001776"
     CDS             828..1376
                     /gene="nusG"
                     /locus_tag="VAH76_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO1386392.1"
                     /translation="MVEENKAKQWYVVHAYSGYENFVMREITSRAKHHNLEDKIGEVV
                     VPSEEVVEMRSGQKRKSTRKFFPGYVLVHMVMDDQTWHMVRGIPRVLGFIGGTSQTPT
                     PISDKEAQAIMQRVEDGVTKPRPKILFEPGEVIRVKEGPFVDFNGVVEEVNYEKNRLR
                     VAVLIFGRSTPVELEFSQVEKT"
     gene            1486..1920
                     /gene="rplK"
                     /locus_tag="VAH76_001777"
     CDS             1486..1920
                     /gene="rplK"
                     /locus_tag="VAH76_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO1386393.1"
                     /translation="MAKKVEAYIKLQIPAGKANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQQMEQGLPIPVVITVYSDRSFTFITKTPPASVLLKKAAGIQSGSGTPNTKKVAKLN
                     VSQLEEIAKVKKPDLTAADLAAAVRSIAGTARSMGIEVEGLE"
     gene            1930..2625
                     /gene="rplA"
                     /locus_tag="VAH76_001778"
     CDS             1930..2625
                     /gene="rplA"
                     /locus_tag="VAH76_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO1386394.1"
                     /translation="MSKLTKKQKKIAEVIKPNQLYTVADAVAILKQFASKKFRESLDI
                     SINLGVDPRKSDQVVRSSTNLPKGTGKTVRVAVFAQGDNAAKATAAGADIVGFEDLAD
                     KIKAGEMNFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTTNVEAAVNDAKSGQ
                     VRYRTDKNGIIHCTVGKADFAPEDVLENVVALINDLKKAKPASSKGQYLKKISLSTTM
                     GPGLPIDISSIPV"
     gene            2798..3331
                     /gene="rplJ"
                     /locus_tag="VAH76_001779"
     CDS             2798..3331
                     /gene="rplJ"
                     /locus_tag="VAH76_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO1386395.1"
                     /translation="MTLNLAAKKAVVEEVTAVASKAISAVVADYRGLTVNQMTQLRSE
                     ARKSGVYLRVVRNTLTRRAFKNTEFECLNDLLVGPVFIALSLEAPSDAARLLKDYAKT
                     FEKLEIRALSVGGKVYNANQIDAVASLPTRDEAISKLMYVMKAPIEKFVRTLAEPHAK
                     LARTLAAVKDKKAGNPA"
     gene            3362..3742
                     /gene="rplL"
                     /locus_tag="VAH76_001780"
     CDS             3362..3742
                     /gene="rplL"
                     /locus_tag="VAH76_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO1386396.1"
                     /translation="MAVSKNEILETISNMTVMEVVELIEAMEEKFNVSAAAAAVAVAA
                     PAAGAGAAAAEEQTEFTVVMTSFGSNKVNVIKAIRGITGLGLKEAKDLVEGAPSTVKE
                     GVSKDEAASIKKELEEAGATVEVK"
     gene            3834..7940
                     /gene="rpoB"
                     /locus_tag="VAH76_001781"
     CDS             3834..7940
                     /gene="rpoB"
                     /locus_tag="VAH76_001781"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO1386397.1"
                     /translation="MAVAEAKPQYSHAEKKRFRKSFGKQTDIMPIPNLLEIQLKSYRD
                     FLQTDTKLSEQLNTGLHAAFSSVFPIESFSGNARLEYVGYKLGEPAFDVRECKLRGLT
                     YSAPLRVKIRLVVLDKDASDDPKPIKDIREQDVFMGEIPLMTDVGTFVVNGTERVVVS
                     QLHRSPGVIFEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVYVRIDRRRKLP
                     VTILLRALGYEAEDILSEFFETTRCHLKNGEYHIDLIPQRLRGEIASFDIHVPETGEL
                     IVEQGRRITARHIKQMEKSQMQDLVVPRDYLIGKTLAKNIIDTSTGEFLAQANDEITE
                     ELLDAMANHGILQIDMIYTNDLDHGSYISDTLKIDPTGSQLEALVEIYRMMRPGEPPT
                     KEAAEALFKNLFFVEERYDLSAVGRMKFNRRVGIKSDEGPGTLTKEDILSVIKTLIDI
                     RNGIGMVDDIDHLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLVESENLMPQDLI
                     NAKPVSAAIKEFFGSSQLSQFMDQVNPLSGVTHKRRVSALGPGGLTRERAGFEVRDVH
                     TTHYGRVCPIETPEGPNIGLINSLSVYARTNEYGFIETPCRKVVNGRVTDEVEYLSAI
                     EEVDQYIAQSNVELDAQGNILADLVPCRHQNEFSLTTPDKINYMDVSPKQIVSVAASL
                     IPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTGMERIVASDSGVSVVAKRGGVID
                     LVDASRIVVRVNDDETTAGETGVDIYNLTKYFRSNQDTCINQRPIVSTGDRIQRGDVL
                     ADGPCTDMGELALGQNLLVAFMPWNGYNFEDSILISERIVHDDRFTTIHIEELTCIAR
                     DTKLGTEEITADIPNVGESALSNLDESGVVYIGAEVKAGDILVGKVTPKGETQLTPEE
                     KLLRAIFGEKASDVKDSSLRVPSGMNGTVIDVQVFTRDGLEKDARAKSIEEEHLARVR
                     KDLIDERRIREEDIYHRVSHLLLDKVATGGPGSLKPGSKITQDYLDKVEREKWFDIRI
                     EDDAVSQQLEQLSKQLELLTKEMEKRFNDSRKKIIQGDDLAPGVLKIVKVYLAVKRRI
                     QPGDKMAGRHGNKGVISIVVPVEDMPHMEDGTAVDIVLNPLGVPSRMNIGQVLETHLG
                     LAAKGLGRKIAQMLDERQTPEAIKAYLEKIYNHDGVQRVNLKCLNDDELMTLADNLRA
                     GVPMATPVFDGATEQEIKSMLQLADLPADGKTVLIDGRTGNKFDNPVTVGYMYMLKLN
                     HLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVK
                     SDDVGGRTKIYKNIVDGDHRMDPGMPESFNVLLKEIRALGIDIELEHD"
     gene            8029..12234
                     /gene="rpoC"
                     /locus_tag="VAH76_001782"
     CDS             8029..12234
                     /gene="rpoC"
                     /locus_tag="VAH76_001782"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032829172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO1386398.1"
                     /translation="MSDLLGILKQQGQSEEFDAIKIALASPELIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKTFGPVKDYECLCGKYKRLKHRGVICEKCGVELALAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEAFVVVDPGMTELERGQLL
                     NDEAYLDAMEQYGDEFDARMGAEAIRDLLRQIDLEDEIRNLREELPTTNSETKIKKIT
                     KRLKLLEAFYESGNKPEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLEFRGLAT
                     TIKAAKKMVEREESVVWDILDDVIREHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLEARSLMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYLTREKVNALGEGKIYSSAQEAQNFYEAGHLDIHAKIKIRMPKEDGETGYHLVET
                     TVGRAILAEILPKGMPFDYINRTMTKKVISKVIDSCYRKFGLKETVIFADQLMYTGFK
                     YATRSGASIGIEDMEIPDDKASIIEHADNEVREIESQFRSGLVTNGERYNKVIDIWSR
                     TNELVAKSMMSKIATEEVTDAKGNKVRQESFNPIFMMADSGARGSAAQIRQLAGMRGL
                     MAAPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRRLVDVA
                     QDVVITEDDCGTDTGILMQPLIEGGDIVEPLHERVLGRVVASDVYIPTQTEPVVKAGT
                     LLDEEWVEKLEKHGVDQVMVRSPITCQTRFGLCAKCYGRDLARGHLVNTGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGAASRATAANNIQIKTKGVIRLHNIKTVTHENKNLVAVSR
                     SGEVTIVDEFGRERERYKVPYGAVISAQDNSPVEAGQVIATWDPHTHPVISEVSGRLK
                     FVDLIDGITMNRQTDELTGLSNIVIIDAKQRSAAGRDLRPMVKLVTDEGDDIYLAGTN
                     VPAQYYLPVDAIVNFEDGSLVGIGDVIARIPQERSKTRDITGGLPRVADLFEARKPKD
                     SAVMAEVSGLVNFGKETKGKRRLIINVSEDQCHEELIPKWRHISVFEGEHVERGEIIA
                     EGALNPHDILRLLGVGALANYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKRVITFA
                     GDSKFLVGEQVEESAMLQENDKLLAEGKQIARGTPILLGITKASLATESFISAASFQE
                     TTRVLTEAAVSGKVDELRGLKENVMVGRLIPAGTGYTYHQSRKAKRARAAAGGDSSAT
                     HTVTASDVEHALSEALNADNHEH"
     gene            12348..12728
                     /gene="rpsL"
                     /locus_tag="VAH76_001783"
     CDS             12348..12728
                     /gene="rpsL"
                     /locus_tag="VAH76_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO1386399.1"
                     /translation="MATINQLVRKPRVDVKKKSNVPALESCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVNDRKQGRSKYGTKKPKDKK"
     gene            12749..13276
                     /gene="rpsG"
                     /locus_tag="VAH76_001784"
     CDS             12749..13276
                     /gene="rpsG"
                     /locus_tag="VAH76_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO1386400.1"
                     /translation="MPRRREVPKREILPDPKHHSELLAKFINVLMVSGKKSIAEKITY
                     GALSVMEERVKKIKKNEEDGSETGSSGSAGAVLRYFEEALDNVRPSVEVRSRRVGGAT
                     YQVPVEVRHDRSIALGMRWIVQAARTRGEKGMMLRLAGELMDAYENKGSAVKKREDTH
                     KMAKANQAFAHFRWN"
     gene            13291..15375
                     /gene="fusA"
                     /locus_tag="VAH76_001785"
     CDS             13291..15375
                     /gene="fusA"
                     /locus_tag="VAH76_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO1386401.1"
                     /translation="MATPLKLYRNIGIAAHVDAGKTTTTERVLYYTGMSHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCYWSGMDKQFESHRINIIDTPGHVDFMIEVERSLR
                     VLDGAVVVFDSVAGVEPQSETVWRQANKYGVPRIVFVNKMDRMGANFLRVVSQIKQRL
                     GSTPVVLQLPIGAEEEFKGVIDLIKMKAIHWDEENKGMTFKYVDIPADLKATCEEYRA
                     HIIEAAAEYSEELMEKYLEGEEFTEAEIKKALRHLTITNKVVPVFCGSAFKNKGVQAV
                     LDGVIEYLPSPTDIPDIQGVDEHGDEIHRKTSYDEPFSALAFKIATDPFVGTLTYFRA
                     YSGILKSGDTVYNSVKGKKERIGRLLQMHANSREEIKEVRAGDIAAAVGLKTVTTGDT
                     LCDQDKVVILERMDFPDPVIAVAVEPKTKADQEKMGIALGKLAQEDPSFRVHTDEESG
                     QTIIQGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETLKQAVEQEGKFVRQSGGRG
                     QYGHVWLKIEPQEPGKGYEFINAIVGGVIPKEYIPAVDKGIQEQMQNGVIAGYPVVDV
                     KVTLFDGSFHEVDSSEMAFKIAGSQCFKQGALKAKPVLLEPIMSVEVVTPEDYMGDVM
                     GDLNRRRGLVQGMEDSPAGKIVRAEVPLAEMFGYSTDLRSATQGRATYTMEFCKYAEA
                     PTNIAEAIIKKQ"
     gene            15396..>15901
                     /locus_tag="VAH76_001786"
     CDS             15396..>15901
                     /locus_tag="VAH76_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEO1386402.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTA
                     KAYDQIDAAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
                     YDFPGDDIP"
BASE COUNT         5127 a         2784 c         3734 g         4256 t
ORIGIN      
        1 aagccaggaa ctatcaagcc acacaccaag tttgaagcag aagtgtatgt attatccaaa
       61 gaagaaggcg gacgtcacac tccattcttt aatggatacc gtccacaatt ctatttcaga
      121 accactgacg tgacaggtac ttgtgacttg ccatcaggag ttgaaatggt aatgcctgga
      181 gataatgtgc aattagttgt tagcttgcac gctccgattg cgatggatga aggtttaaga
      241 ttcgcaatta gagagggtgg ccgaactgtt ggcgccggtg tagtcgctaa aataatcgag
      301 taaaatagga aaaagtacaa aagtggcatg agccttatat acatggctca tgtcaataaa
      361 atttaggcca gtagctcaat tggcagagtg gcggtctcca aaaccgcagg ttgggggttc
      421 gattccctcc tggcctgcca actacaagta aatatgaaaa gttcaaacga aaatcaaggt
      481 aagatgaaag atataatgtc ctggctagct atcatattag ctacagcagg agcttttgta
      541 tgtacttact attatacgtt cacaggacca attcaatcga taatatggct tgcttggctt
      601 attttgatcc ttttcttggg ttacttaaca acaacaggta aaagagtctt tgtgtttgcg
      661 caagaagcaa aagttgaatt attaaaagtg gtttggccta cacggcaaga aacaatacaa
      721 acaactacta tagtaatggt gatggttgga ttgacaggat ttatactctg gggagtagat
      781 tcaatcatga tgtgggcaat tgctaagtta acacatttag ggtgaatatg gtcgaggaaa
      841 acaaagcaaa acagtggtac gttgtacatg cctattcagg atatgaaaat tttgtcatgc
      901 gcgaaataac atctcgtgca aaacaccaca atttagaaga taaaataggc gaggtagttg
      961 taccttcaga agaagttgtt gaaatgcgtt caggccaaaa gcgtaaaagt acccgcaaat
     1021 ttttcccagg atatgtactc gttcatatgg taatggatga tcaaacatgg cacatggtca
     1081 gaggcatacc cagagttcta ggctttattg gaggaaccag ccaaacccca actccgattt
     1141 ctgataaaga agctcaagcc attatgcaac gagttgaaga tggcgtaact aagccaaggc
     1201 caaaaatact atttgagcct ggagaagtaa tcagagtgaa agaaggtcca tttgttgact
     1261 ttaatggagt tgttgaagag gtcaattatg agaaaaacag actaagagtc gcagttctta
     1321 tttttggacg ttcaactcca gtagaacttg agttcagtca agtagaaaaa acataaatga
     1381 tttagataaa atttaaattt aaaggtttta caaaaccggg gagctaagac ttaattgaaa
     1441 atgttaattc aataaaagct agcgcatgac ccataaggag taataatggc aaaaaaagta
     1501 gaagcatata ttaaattaca gatccctgca ggaaaggcaa atcctagccc tccagttgga
     1561 cctgctttag gtcaacgtgg ggtaaatatt atggaattct gtaaagcatt caatgctgcg
     1621 acacagcaaa tggaacaagg tctgcctatt ccagttgtta tcactgtata tagtgatcgt
     1681 agttttacct ttataaccaa gacccctcca gcttcagttc tgctgaaaaa ggctgctggc
     1741 attcaaagcg gaagtggcac accaaatact aaaaaagtgg ccaaactgaa tgttagtcaa
     1801 cttgaagaaa ttgctaaggt caagaagcct gatctgacag cggctgatct agcagcggct
     1861 gtaagaagta ttgcaggtac agcacgtagc atgggtattg aagttgaagg tttggaataa
     1921 ggggttacca tgtcaaaatt aactaagaaa caaaagaaaa tagcggaagt tattaagcca
     1981 aatcaactct atacagttgc tgatgctgta gctattctaa agcaatttgc atcaaaaaaa
     2041 tttcgtgaga gccttgatat cagtattaat ttaggagttg atcctcgtaa atcggatcaa
     2101 gtagtacgat cttctaccaa tctgccaaaa ggtacaggaa aaacagtacg agttgccgtt
     2161 tttgcccagg gtgataatgc tgctaaagct actgctgcag gtgccgatat agtaggattt
     2221 gaagatctgg ctgataaaat caaagcaggc gaaatgaatt ttgatgttgt tattgcaact
     2281 ccagatgcta tgagaatcgt aggtcagctc ggacagattt tgggaccaag gggactcatg
     2341 cccaatccta aggttggaac agtaacaact aacgtggaag cagcagttaa tgatgccaaa
     2401 tcaggacaag taagatacag aactgataaa aatggaatta ttcattgtac tgttggaaag
     2461 gcagattttg ctcctgaaga tgttcttgaa aacgtagttg cattaatcaa tgatctgaag
     2521 aaggcaaagc cagcatcctc taaagggcaa tatctgaaga aaatatcttt atctacaaca
     2581 atgggacctg ggttacctat tgatatttcg tcaatacctg tgtaacatat catcccattt
     2641 ttaagaattc acggcataga ttaggttcca tgccgtcgaa gaccgcaggt gcctaggctt
     2701 aatttcctgc gcagacggtg aaccggctcc ttaataaaga gacggccacc ataactgtca
     2761 agtccatgag atttgtacat attaggaggt cagtaacgtg acattaaatt tagctgcaaa
     2821 aaaagccgtt gttgaagaag tgactgcagt cgcgtcaaag gctatttcag cagtggttgc
     2881 tgattatcgt gggttaactg taaatcaaat gacgcaattg cgtagtgaag cacggaaatc
     2941 cggtgtttat cttcgtgtgg tgcgaaatac actaacaaga agagcgttta aaaacactga
     3001 atttgaatgc ttgaatgact tacttgtagg tccagtattc attgcattgt cactggaagc
     3061 accaagtgat gctgcaaggc tactgaaaga ttacgcaaaa acatttgaaa aacttgagat
     3121 tagagcatta tcagttggcg ggaaggtata caacgcaaat caaattgatg ctgttgcgag
     3181 cttgccaaca cgcgatgagg caatatccaa gttaatgtat gtgatgaaag cgccaattga
     3241 gaaatttgtt cgtacacttg cagagccaca tgccaagttg gctagaactc ttgctgctgt
     3301 aaaagacaaa aaagcaggca atcccgctta atcattattt aattttaatt aggagctagt
     3361 aatggctgta tcaaaaaacg aaattttaga aacaatttct aacatgacag taatggaagt
     3421 tgttgaatta atcgaggcaa tggaagaaaa atttaatgtt tctgctgccg ctgctgctgt
     3481 agctgttgca gcgcctgctg caggtgctgg tgccgctgct gctgaagaac aaaccgaatt
     3541 tactgttgta atgaccagct ttggttctaa caaggtaaac gtaatcaagg caattcgtgg
     3601 cataactggt ctgggcttga aagaagctaa agacttagta gaaggtgcac catcaactgt
     3661 taaagaaggt gtatcaaaag atgaagctgc cagtatcaag aaagagcttg aagaagctgg
     3721 tgctacagta gaagttaaat aattaaaata tagcagtatt taagaaccca gccatgttca
     3781 catggctggg tttactcgtt tgaaaatcat caataattta cagaggaacc accatggcag
     3841 ttgcagaagc taaacctcaa tattcgcatg ctgagaaaaa acgatttcgc aaaagctttg
     3901 gtaagcagac agatataatg ccaataccga atctgcttga aattcaactt aaatcctaca
     3961 gggattttct tcaaactgat actaaattaa gcgaacaact gaatacggga ttgcatgctg
     4021 cattttcttc tgtatttcct atcgaaagct tttctggtaa tgcaagactt gaatacgtcg
     4081 gatacaagct tggagagcct gcatttgatg ttcgcgaatg caaactaaga gggctgactt
     4141 actcagctcc tctaagagtc aagatcagac tcgttgtgct tgataaagac gcaagcgatg
     4201 atcctaaacc tataaaggat attagagagc aagatgtttt tatgggggaa attcctctaa
     4261 tgaccgacgt tggtacgttt gtggtcaatg gaactgaacg tgttgttgta tcacaattac
     4321 atcgttcgcc tggggttata tttgaacatg acaagggaaa aactcactct tcaggtaaat
     4381 tgctctattc tgcacgaata ataccttatc gtggttcatg gttggatttt gaattcgatc
     4441 ctaaagattg cgtttatgta cgtattgaca gacgtcgcaa actgccagtg accattttgc
     4501 tcagagcatt gggctacgaa gcagaagata tcttaagcga attttttgaa acaactcgtt
     4561 gtcatttaaa aaatggcgaa taccacatcg atttaattcc acagcgtttg cgtggtgaaa
     4621 tcgcttcatt cgatattcat gttcctgaga caggagagct tatcgtagaa caaggtcgtc
     4681 gaattaccgc aagacatata aagcaaatgg aaaaaagcca aatgcaggat cttgtggtgc
     4741 caagagacta tttaattgga aaaacattag caaaaaatat tattgatacc tcaaccggtg
     4801 aatttctggc tcaagcgaat gatgagataa cggaagagct tttagatgct atggctaacc
     4861 atggtatttt acaaattgat atgatctata ctaatgattt ggatcatggt tcttacatat
     4921 cggatacatt aaaaattgat cccacaggat cacaactaga agcactcgtt gaaatttacc
     4981 gcatgatgcg ccctggagag cctcctacaa aagaagctgc tgaggcactg ttcaaaaatc
     5041 tattctttgt tgaagaaaga tatgatctat ctgctgttgg cagaatgaaa tttaatcgtc
     5101 gcgtaggaat taagagtgac gaaggcccag gcactttaac gaaagaagat attttatctg
     5161 tcattaaaac actgatagat attcgaaacg gtatagggat ggttgatgat atcgatcacc
     5221 taggaaatcg tagggtaaga agtgttggcg aaatgaccga aaatcagttt agagtaggtc
     5281 ttgttcgtgt tgaacgtgct gttaaagagc gtttaagttt ggttgaatct gaaaatctga
     5341 tgccacagga cttaattaac gcaaaaccag tatccgccgc aattaaagag ttttttggat
     5401 ccagtcagtt atcacagttt atggatcaag tgaatccatt atctggtgtt acgcataaaa
     5461 gacgtgtatc ggctcttggg ccaggtggtt taacacgtga gagggctgga tttgaggtcc
     5521 gtgacgtaca tacaactcac tatggtcgtg tgtgtccaat tgaaacgcct gaaggaccaa
     5581 atattggtct aatcaattca ctgtcagttt atgcaagaac taacgaatat ggatttattg
     5641 aaactccttg tcgaaaggtc gttaatgggc gtgtgacaga tgaagttgaa tacctatcgg
     5701 ctattgagga agttgatcaa tacattgcac aatccaatgt tgagttagac gcacaaggaa
     5761 atattcttgc tgacctagtt ccttgcagac atcaaaatga attttcactg actaccccgg
     5821 ataaaattaa ctacatggat gtctcgccca agcaaattgt gtcagttgct gcttcattga
     5881 ttccgttttt agagcacgat gacgcaaaca gagcattaat gggttcaaac atgcaacgac
     5941 aggctgtacc aaccttgcgt tcagaaaagc ccttggttgg gaccggtatg gaaagaattg
     6001 ttgcatctga ttcaggggtc tcagttgttg cgaagagagg tggagttatt gatctggttg
     6061 atgcttctcg tattgttgtt cgagtgaatg acgacgaaac aacagcaggg gaaacagggg
     6121 tagatattta taacttaacc aagtatttcc gttctaacca ggatacttgt atcaatcaaa
     6181 gacctatagt atcaacaggt gatcgaattc aacgaggcga tgtcctggct gatggtccat
     6241 gtacagatat gggtgaattg gctcttggac agaatctact ggtagcgttt atgccttgga
     6301 atggttataa ctttgaggat tccattctta tatctgaacg aatcgttcat gacgacagat
     6361 tcactactat tcacatcgaa gagttaacat gtatagctcg agataccaaa ttaggtacag
     6421 aagaaattac agcagatatt cccaatgtag gcgagtctgc actctccaat ttggatgaat
     6481 ctggagttgt ttacatcggt gcagaagtaa aagcaggcga tattcttgtg ggtaaagtaa
     6541 ctcctaaagg cgaaacccaa ctaactccag aagaaaaact gttaagagct atctttggtg
     6601 aaaaagcttc agatgttaaa gattcatctt tgagagttcc atcaggcatg aatggaacag
     6661 tgatcgatgt acaagtcttc actcgtgatg gacttgaaaa ggatgcccgc gctaaaagca
     6721 ttgaagaaga gcatctagca cgagttcgca aggatttaat agacgaacga cgtattcgtg
     6781 aagaagacat ctatcataga gtttctcatt tgttgttgga taaggtggca acaggcggtc
     6841 ctggaagctt aaagccagga tctaaaatca cccaggatta cttagataag gttgaaagag
     6901 aaaagtggtt tgatattcgt atagaagatg acgcagttag tcagcaactt gaacaattat
     6961 ccaagcagct ggaactttta acaaaagaaa tggaaaagcg ctttaacgac agtagaaaga
     7021 aaattatcca gggagatgat ttagctcctg gtgtattaaa aattgttaaa gtgtatttgg
     7081 ctgttaaaag aagaattcag cctggagaca aaatggcagg tcgtcatgga aacaagggtg
     7141 tgatctccat tgttgttcca gttgaagata tgccacatat ggaagatgga acggctgtag
     7201 atatcgttct taatccattg ggcgtaccat cgcgaatgaa tatcggtcag gttcttgaaa
     7261 cgcatttagg actagctgcc aaaggattag gaagaaagat tgcacagatg cttgatgaaa
     7321 ggcaaacacc tgaagcaata aaagcatacc ttgagaaaat ttataatcat gacggagtgc
     7381 agcgtgttaa tttgaaatgc ctcaatgatg atgaattgat gacactggct gataacctga
     7441 gagctggtgt gccgatggca actcccgtct ttgatggtgc tactgagcaa gaaatcaaaa
     7501 gtatgttgca gttagcagat ttaccagcag atggtaaaac tgtgttgata gatggccgaa
     7561 caggtaataa atttgataat ccggttactg taggctacat gtatatgttg aagttaaacc
     7621 atttggttga tgataagatg catgctcgct caactggatc atacagtttg gtcactcaac
     7681 aaccacttgg cggtaaagca cagtttggtg gtcagcgttt tggtgagatg gaagtatggg
     7741 cattagaagc atatggtgca gcatacactt tacaagaaat gttaaccgta aaatcagatg
     7801 acgtcggagg tcgaacaaag atctataaaa atatagtcga tggagatcat cgtatggacc
     7861 caggaatgcc tgaatccttc aatgtattat tgaaggaaat tagagcactg ggaattgata
     7921 tcgagttgga gcatgactaa ctcatcagcg atacatactg actaaccatt ttttggaggc
     7981 atagacttgg ctaccctaag caacgaccca atgaaaaaag caccagtaat gagtgactta
     8041 cttggtattc ttaagcaaca aggtcaaagt gaagaatttg atgcaataaa aattgcattg
     8101 gcgtcacctg aattaattcg atcatggtcc tacggcgaag ttaaaaagcc tgaaacaatc
     8161 aactacagga catttaagcc tgaaagagat ggtttgtttt gtgctaaaac ttttggtcct
     8221 gttaaagatt atgaatgttt gtgtggaaag tacaagcgat taaagcatcg tggtgttatt
     8281 tgcgagaagt gtggggttga attggctctt gccaaagtga gacgagaaag aatggggcat
     8341 atcgaactcg ctagcccagt cgctcacata tggttcttaa aatctttacc atcaagaatt
     8401 ggtttgctcc tcgatatgac tctaagagat atcgagagag tactctattt tgaagctttt
     8461 gtcgttgttg atcctgggat gacagagctt gaaagagggc aactacttaa cgacgaagcc
     8521 tatcttgatg cgatggaaca gtatggtgat gagtttgacg cgcgtatggg cgcagaagca
     8581 attcgtgatt tattgcgcca aattgatctc gaagatgaaa ttagaaattt acgtgaagaa
     8641 cttccaacaa ctaactctga gacgaagatt aaaaaaatca ccaaaagact caagttatta
     8701 gaagcttttt acgagtcagg aaataaacca gaatggatga tcatggatgt acttccagtg
     8761 cttccacccg atttgaggcc attggttccg ttagatggtg gacgttttgc tacttcagac
     8821 ttgaatgatt tatacaggcg cgtcattaat aggaacaacc gactaaaacg cctgctggat
     8881 ctgaatgcgc ctgacattat cgttcgtaac gaaaaacgaa tgctgcaaga atccgtagat
     8941 gctttacttg ataacggcag aagaggtcgt gccatcactg gtacaaataa gagacctctt
     9001 aaatcactgg cagatatgat taaaggtaaa cagggtcgtt tcaggcaaaa tctcttaggt
     9061 aagcgtgtcg actactctgg acgttcagtt attgtagttg gaccaacttt aaaattgcac
     9121 caatgcggat tgcctaaaaa aatggcgttg gagttattca agccatttat attttccaaa
     9181 ttggaattta gaggtttggc aactactatc aaagcggcca agaaaatggt cgaaagagaa
     9241 gaatcggtgg tttgggatat attagatgat gttattagag aacatcctat ccttttaaac
     9301 cgtgctccta ccttgcatag attaggtatt caagcatttg agcctgtttt aattgaaggt
     9361 aaagcgatcc agctccatcc tttggtatgt acagcataca atgctgactt cgatggagac
     9421 caaatggctg tgcacgtgcc cttgacactg gaagcgcagc tggaagctcg ttcattgatg
     9481 atgtctacaa acaatatatt atctccagcc agtggtgagc caattattgt tcccagtcag
     9541 gacgtcgtcc ttgggttata ctatttaaca cgagaaaagg ttaatgcatt aggcgaagga
     9601 aaaatatatt caagtgctca ggaagcgcaa aatttctatg aagcaggtca tttggatatc
     9661 catgcaaaaa ttaaaattcg tatgcctaaa gaagatggtg aaactggata tcatttggtt
     9721 gagacaacag tcggacgcgc aattttagct gagattttgc ctaaaggaat gccatttgac
     9781 tatataaacc gtacaatgac aaaaaaggtt atctcaaaag taatagacag ctgttaccgc
     9841 aaattcggct tgaaagaaac agtcattttt gctgatcagt taatgtatac cgggtttaaa
     9901 tacgccacac gatctggagc atccataggt attgaagaca tggaaatacc agatgataag
     9961 gccagtatta ttgaacacgc cgataatgaa gtaagagaaa tcgagtctca attcagatcg
    10021 ggtttggtaa cgaatggtga acgatacaac aaggtaattg atatttggtc aagaactaat
    10081 gaattagttg ccaaatcaat gatgtctaaa attgcaacag aagaagtcac tgatgcaaaa
    10141 ggcaacaaag taagacaaga atcatttaac ccaattttca tgatggctga ttctggagct
    10201 agaggttctg ctgctcagat acggcagctt gccgggatgc gaggtttgat ggcagctcca
    10261 gatggctcaa tcattgaaac gcctatcact gcaaacttcc gcgaaggttt gaatgtattc
    10321 cagtacttta tttctacaca cggagctcgt aagggtttgg cagatacggc gttaaagaca
    10381 gcaaactcag ggtatctgac acgacgcttg gtagatgttg cgcaggatgt tgttattacc
    10441 gaagatgatt gtggtactga tacaggtatt ctaatgcaac cactgattga aggtggtgat
    10501 attgttgagc ctttgcatga acgcgtcctt gggcgggtgg ttgcttcaga tgtctatatt
    10561 cctactcaaa ctgaaccagt agtcaaggct ggaactttac tggatgaaga atgggttgaa
    10621 aaattagaga agcatggggt agaccaggtt atggtcaggt caccaatcac ttgtcaaaca
    10681 agatttggtc tttgtgcaaa atgctatggt agagatcttg ctcgtgggca tcttgttaac
    10741 acaggtgaag cagttggtat tatcgcagca caatcaatcg gtgagccagg aacacagtta
    10801 accatgcgta ctttccacat tggaggtgct gcatcaaggg caacagctgc gaataatatt
    10861 caaattaaaa cgaaaggggt tattcggtta cataatataa agaccgtaac tcatgagaat
    10921 aaaaacctgg tggcagtttc tcgctcaggt gaggtaacta ttgtcgatga gttcggacga
    10981 gaaagagagc gctataaagt tccttatggt gcagtaattt cagcacagga taattcccct
    11041 gttgaagctg gtcaggttat tgcaacgtgg gatccccata cgcatccggt tatttccgaa
    11101 gtgtcagggc gtttgaagtt tgttgatctt attgatggca ttacaatgaa tagacaaact
    11161 gatgaattaa ccgggttaag caatattgta ataattgatg ccaaacaacg gagtgcggca
    11221 ggtcgagatt tgcgcccaat ggtaaaatta gttacagatg agggggatga catatatctt
    11281 gctggaacca atgtcccggc acaatattat ctacctgtcg atgccattgt gaactttgaa
    11341 gatggaagtc tggtgggaat aggggacgtt attgcccgta ttccgcagga acgttctaaa
    11401 acccgagata ttaccggagg tttgccaaga gtagcggact tatttgaagc cagaaaacca
    11461 aaggattctg cagtaatggc agaagtatca ggattggtta attttggtaa agaaaccaaa
    11521 ggtaagcgac gcttgattat caatgtatcg gaagatcaat gccatgaaga gctgattcct
    11581 aaatggcgtc atatttcagt gtttgaaggt gaacacgtag agcgcggtga aatcatcgca
    11641 gagggtgcac ttaatcctca tgatatattg cgcttattag gtgttggagc gctggctaat
    11701 tacattgtaa atgaggtcca ggacgtctat cgtttacaag gtgtgaaaat taatgataaa
    11761 cacattgaag taattgtcag acaaatgcta cgtaagagag tcattacttt cgctggtgac
    11821 tcaaaattcc tcgtaggaga gcaagtagaa gaaagtgcta tgcttcagga aaatgataaa
    11881 cttctagctg aaggaaagca gattgcgcga ggtactccta ttttactagg tatcacaaaa
    11941 gcttcattag ctacagaatc gtttatatca gccgcttcat tccaagagac aaccagagtc
    12001 ttgactgaag cagcagtaag cggaaaagtt gatgaattgc gtggtttaaa agaaaacgtg
    12061 atggtaggtc gactaattcc agcaggaaca ggttatactt atcatcaaag ccgaaaggca
    12121 aaaagagcaa gggctgcagc aggcggagat agttcagcaa cacacacagt tactgcaagt
    12181 gatgttgaac acgcgttgag tgaagcatta aacgcggata atcatgaaca ttaatcagga
    12241 ttcaaaaatg gcagatttaa gcttgacaaa tctgccgtac ctatatagaa tccggcctcc
    12301 ttatgcattc aaaatattca caagtgacag atcggagtta agtacaaatg gctactatta
    12361 atcagttagt aagaaagcct cgtgtggacg taaagaagaa aagcaacgtg ccagcacttg
    12421 agtcatgtcc acaaagacgc ggtgtatgta ctcgtgtata cactactaca ccaaaaaaac
    12481 ctaactcagc tatgcgtaag gttgcacgtg tgcgcttgac caatggattt gaagtaacat
    12541 cctatattgg tggtgagggt cataatttac aagagcactc tgttgtttta attagaggtg
    12601 gtcgtgttaa ggatttacct ggtgtgcgct atcatacagt tcgaggtagc ttagatactt
    12661 caggagtgaa cgaccgaaaa caagggcgtt ccaagtatgg tacaaaaaag ccaaaagata
    12721 aaaaataacc gattgtagga aaattgagat gccaagaaga cgagaagtac ccaaacgaga
    12781 aattttacct gatccaaaac atcatagtga actgcttgct aagttcatta acgttttaat
    12841 ggttagcggt aaaaaatcaa ttgctgaaaa aattacttat ggtgcgctgt ctgttatgga
    12901 agagcgtgtc aaaaaaatta agaaaaatga ggaagatgga agtgaaactg gcagcagcgg
    12961 aagtgccggt gccgtacttc gttattttga agaggcttta gataacgttc gcccaagcgt
    13021 ggaagtacgt tcacgccgag ttggtggcgc aacataccaa gttccagtag aagtccgaca
    13081 tgatagaagc atagcgcttg gaatgcggtg gatagttcaa gctgctcgca cacgtggtga
    13141 aaaaggcatg atgctgcgtt tggcagggga actgatggac gcttatgaaa ataaagggtc
    13201 tgcagtaaag aaacgtgaag atacacataa aatggcaaaa gctaaccaag cctttgctca
    13261 ttttagatgg aattaagaga gagataagcc gtggcaactc cattaaaact atatagaaac
    13321 ataggcattg cagcccacgt tgatgctgga aaaacaacaa ctacagaacg agtattgtac
    13381 tataccggaa tgtctcataa gattggtgag gtacatgatg gtgctgcaac tatggactgg
    13441 atggtccagg agcaggaacg tggtattacc attacttctg ctgcaacaac ttgttattgg
    13501 tctggtatgg acaaacaatt tgaatcccat cgaatcaata taattgatac accaggacac
    13561 gtagacttta tgattgaagt cgaacgctct ttacgtgtac tagatggagc agttgttgta
    13621 tttgattcag ttgccggcgt tgaacctcaa tctgaaacag tatggcgtca agccaataag
    13681 tatggtgttc ctcgtatcgt ttttgtaaat aaaatggata ggatgggtgc caatttccta
    13741 agagttgtga gccagattaa gcaacgatta ggctctactc ctgttgtgtt acaattacca
    13801 attggcgcag aagaagagtt caaaggtgtg attgatctca ttaaaatgaa ggcaattcac
    13861 tgggatgaag aaaataaagg tatgacattt aaatatgttg atatccctgc tgatttaaag
    13921 gcaacctgtg aagaataccg tgcacacatc attgaagcag cagcagaata ctctgaagag
    13981 ttaatggaaa aatatttgga aggcgaagaa ttcacagagg cggaaattaa aaaggctctt
    14041 cgtcatctga caatcacaaa taaagttgtt cctgtattct gtggatcagc ctttaagaat
    14101 aagggtgtcc aagcagtact cgatggagta atagaatatt taccatcacc cactgacatt
    14161 cctgatattc agggagttga tgagcatggt gatgaaatcc atagaaaaac cagttatgat
    14221 gaaccattct cagcgttagc gttcaaaatt gcgactgacc catttgtggg cacgctaaca
    14281 tattttagag cttattctgg tattcttaag agtggggata cagtctataa ctcagttaag
    14341 ggtaaaaagg aacgtatcgg ccgcttgttg cagatgcatg ctaattctcg tgaagaaatt
    14401 aaagaagtgc gagcagggga catagccgct gctgtaggtc ttaaaacagt aactactggt
    14461 gataccctct gtgatcaaga taaagttgta atattagaga gaatggattt cccagatcca
    14521 gttattgcag ttgctgttga accaaaaact aaagcggatc aggaaaaaat gggaattgct
    14581 ttaggcaagt tagcccagga agatccttct ttcagagtac atactgatga agagtcaggc
    14641 cagacaatca ttcaaggtat gggtgagtta caccttgaaa ttattgttga ccgcatgaag
    14701 agagagttca atgtagaagc taatgttggt aaacctcagg ttgcataccg agagacactc
    14761 aagcaagctg ttgaacaaga gggtaagttc gtaagacaat caggtggacg tggtcaatat
    14821 ggacatgtgt ggttaaaaat tgaaccacaa gaaccaggaa aaggttatga atttatcaat
    14881 gctatcgttg gtggcgtcat tccaaaagaa tatatccctg cagtagataa agggattcaa
    14941 gagcaaatgc aaaatggtgt aattgctgga tatccagtgg ttgatgttaa agttacactg
    15001 tttgatggtt cattccatga agttgattca agcgaaatgg catttaaaat tgctgggtct
    15061 caatgcttca agcaaggtgc actaaaggcg aaaccggtac ttcttgagcc aattatgagt
    15121 gtcgaagttg ttacacccga agattacatg ggcgatgtta tgggtgatct gaacagacgc
    15181 cgtggtttag ttcaaggtat ggaagactca ccagcaggca aaatagttag agcagaggtg
    15241 ccgcttgcag agatgtttgg ttattcaact gacttacgtt ctgctacaca aggaagagca
    15301 acctacacta tggagttttg taagtacgca gaagcaccaa caaatattgc tgaagcaatt
    15361 atcaagaaac aataaaagtt aacgaggtta ttgaaatggc gaaggaaaaa tttgaacgta
    15421 agaagccgca cgtaaacgtg ggcacgattg gtcacgtaga ccatggcaag acgacattga
    15481 cagcagctat tacaacgatt atggcgaaga aatatggtgg tacagcgaag gcgtacgatc
    15541 aaattgatgc tgcgccagaa gaaagagagc gtgggattac tatttctaca gcacacgttg
    15601 agtacgaatc agcgagcaga cattatgcgc acgtagactg cccggggcat gctgactatg
    15661 ttaagaacat gattactggt gctgcgcaaa tggacggagc gatactggtt gtatcagcgg
    15721 ctgatggtcc tatgccacaa acgagggaac acattctatt gtctcgccag gtaggtgttc
    15781 catatattgt tgtgttcatg aacaaagcgg atatggttga tgaccctgag ttattagagt
    15841 tagtggaaat ggaagtgcga gatttattaa gcagttacga tttcccaggg gatgacatac
    15901 c
//
LOCUS       DATNSN010000072        15794 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.072, whole genome shotgun sequence.
ACCESSION   DATNSN010000072 DATNSN010000000
VERSION     DATNSN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.072"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1551
                     /locus_tag="VAH76_001787"
     CDS             <1..1551
                     /locus_tag="VAH76_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1386403.1"
                     /translation="IQFIDYLMHSCSGQKCEPCIGVRQSDHRFQNTLWEQFPFNMYAQ
                     SFLMNEHIWNEATTAIRGVSEHHENLVNFTTRQILDMFAPSNFPFTNPEVIEATINEG
                     GANFVNGLNNWIEDVSRKLNKQPPVGSEQFKVGKNIAITPGKVVYRNHLIELIQYEPT
                     TPKVYLEPILMIPAWIMKYYILDLSPNNSMVKYLVDQGHTVFMISWRNPDEKDRNLTM
                     EDYISLGAMDAMNAITSIVPKQKIHSVGYCLGGTLLMLAAASIAKEKDNPLKSITLFA
                     SQVDFKDAGELMLFIDESQIAYLEDIMWTKGYLDGQQMAGTFSMLQSIDLIWSKIMLD
                     YQLGKRRPINDLMAWDYDTTRLPYAMHSEYLRKLFLNNDLIEGHYCVNGKILTLQDIE
                     LPIFAESALRDHVAPWKSVYKIHYFTDTDITFVLTNGGHNSGVVNEPGHPGRSYQLMT
                     HKKGDKDIFPELWQQKASQNEGSWWPAWQQWLASYSGKKVLPPAMGNAKRGYKVLCDA
                     PGTYVLQK"
     gene            1578..2318
                     /gene="phbB"
                     /locus_tag="VAH76_001788"
     CDS             1578..2318
                     /gene="phbB"
                     /locus_tag="VAH76_001788"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEO1386404.1"
                     /translation="MKNRIALITGGMGDIGTAICHEFCNRGAKVIAADRLDKDKALAW
                     QAQQKKLGFEMGIASMDVTQFESCVALAKDVEQRFGPIDILVNAAGTINDVTLRKMTP
                     EQWNQVVHTDMDSMFNVTRQFINGMVERGYGRIVNISSVNAEKGQFGQTNYSSAKSGV
                     YGFTKSLAQEVAKHGVTVNTISPGYVESRMVMSVPEPVRLKIIENIPVGRLAKPEEIA
                     WSIAFLASERSGFMTGSNLAVNGGIHMY"
     gene            2356..3549
                     /locus_tag="VAH76_001789"
     CDS             2356..3549
                     /locus_tag="VAH76_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO1386405.1"
                     /translation="MDHKNDPIVIVSAVRTPIGHFGGYFKNCSTIELGATAIKAVIER
                     AKLKPADIQEVLMGCVLPAGLGQAPARQASILAGVPTSVPCVTVNKVCGSGMQTVIFA
                     YDELQAHSRNIIIAGGMENMSRAPYLLPKARFGYRLGHGEILDHMMFDGLEDPYSKKA
                     MGFFAEQCATKYKFSRKEQDDYAIESFHRAKHATDKGYFAAEICPVSVTDKHHNVVTV
                     SQDEGISCVDPEKIPKLHPAFVKDGTVTAGTSSQISDGASALIIMRQSEAEKRGIKPI
                     AKILGHCIFAHDPAWFTTAPIGAINGVLENTGLTTKDIDLYEINEAFAVVPMAAIKEL
                     KLSEKQVNFFGGACVLGHPLGDTGARIIVTLLNALHQKKLHRGIAALCIGGGEATAVV
                     VEMMP"
     gene            3575..4342
                     /gene="fabI"
                     /locus_tag="VAH76_001790"
     CDS             3575..4342
                     /gene="fabI"
                     /locus_tag="VAH76_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEO1386406.1"
                     /translation="MIIDLTNKKGLVTGIANEHSIAYGCAEQFCEANAELATTFLNAK
                     AEPFMHPLALELKSPLILPCDVQKEEEIIALFAAIEKKWGKLDFMLHSMAFAPKTDLE
                     DQLVDCTKEGFLEAMDISCHSLIRLSKYAAPLMKNGGSILTMTYYGSQKVILNYNLMG
                     VVKAALEASVRYLAYELGPKNIRVNAISPGPIQTRAASGIPNFYEYIDAEKQRSPLHR
                     LVDIKNVGNLAAFLASDAAMDMTGQVHYVDAGYSIMS"
     gene            4364..5761
                     /locus_tag="VAH76_001791"
     CDS             4364..5761
                     /locus_tag="VAH76_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEO1386407.1"
                     /translation="MSFIENRTFDELKIGDSAHIERTLTPKDIELFSIMSGDVNPAHV
                     DAEYAKNDMFHQIIVQGMWGAALISTVLGTELPGPGTIYLDQTLRFSAPVVPGDTITI
                     SVTVTDKKPEHHIVVLECKCVNQHDKVVISGVATVMAPTLKVKRERIELPKAVLKEPK
                     GTWYKKLIKLTKDMTPLKTAIVHPVDPLALQGAVDAANEHLIIPILVGPKDKILAAAL
                     EAKLDISAYELIATRHSNESAEVAVKLVKEGKAEALMKGKIHTDELMGPVVNKETGLL
                     TGRRMSHVFYVEAPKYFKPLFLSDAALNIRPSLEEKKDITQNAIDLFHTLGFGTPKVA
                     IVCAVETVNEKMQSTLDAAALSKMADRGEITGAIVDGPLAFDNAISIESAREKNIKSE
                     VAGYADIIIVPDIESGNMLYKEMTYMSGMEAAGMVMGASVPIILTSRGSDDISRKASC
                     AMALIYVRNKEKLHD"
     gene            5754..6932
                     /locus_tag="VAH76_001792"
     CDS             5754..6932
                     /locus_tag="VAH76_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEO1386408.1"
                     /translation="MTEVLLVINTGSSSIKFSIFACQKKLNLLYHGEIESISESPSLT
                     ILDANHTQIVDKKISSKGTEAGLRAFFNWFEHLPDSMKLKAVGHRVVHGGTYFSHPTL
                     VTDEVVKKITSLIPFCPFHEPQSLEAIKSIKTIYPDMPQVVCFDTTFHRTQEHLATLF
                     AIPRKLTDEGLIRYGFHGISYEYIASVITQHIGDIGNKRVIVAHLGSGASMCAMHQRK
                     SVATSMGLTALDGLMMGARSGSIDPGLILYLLQEKKLSVEQVTTLLYQESGLLGVSGI
                     SSDMRKLQSSDEPHAIEAVDLFCFIAARELSALCGILQGCDAIIFTAGIGEKSVLVRK
                     KICDRLNWLGVVLDEKANSTNASVISQDSSKIKVGVIPTNEEYMIAKHTMDGIVETIN
                     "
     gene            6956..9466
                     /locus_tag="VAH76_001793"
     CDS             6956..9466
                     /locus_tag="VAH76_001793"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HEO1386409.1"
                     /translation="MKTSTNKHHAKKSKQNTKNDLKSIPMAELLKKLDTTQNGLSQAE
                     AKKRLIQYGPNELEEKNINPFLKFLTYFWGPIPWMIEVAVILSAVVQHWPDFFIILIL
                     LISNAVVGFWEEYQAGNVIAALKAKLAIKSLVKRDGKWITPPACELVPGDIIRMRLGD
                     IVPADARLLDGDSIEVDQSALTGESLPITRQSGEAVFSGSIIRQGEISALVYATGQKT
                     YFGKTAQLVEEAHTVSHFQRAVLKIGNYLIIFALVMVSGIISVAIYRGDPILTTLQFA
                     LVLTVAAIPVAMPTVLSVTMAVGARLLAKKKAIVSKLVAIEELAGVDVLCADKTGTLT
                     QNKLTLGDPFCAKKFTAEQVILNGALASRVDDNDTIDMAVMAGLKSKDSLKGYKMLHF
                     IPFDPVHKRTEATIKGPDGKEFKVTKGAPQVILKLAANAAQIKADATKAVDEFAARGF
                     RSLGVARAEGEGKWQFVGVLPLFDPPRKDAKKTIATAFAMGVKIKMVTGDALAIAKET
                     AEKLGMGSNILDASTLGDANRKETTKMTKYIENADGFAQVFPEQKFHIVDALQKSGHM
                     VGMTGDGVNDAPALKKADCGIAVSSAMDAARAAASIVLTTPGLSVIIDAIKESRKIFQ
                     RMNSYAIYRIAETLRVLLFMTAAILIFNFYPLTAVMIVMLALLNDGAILSIAYDNVHY
                     KDAPEAWNMRMVLGIASVLGIIGPIAAFGLFYLGDQVFHLTHKHLQPMMYLMLSVAGH
                     MTIFITRTRGPFWSIRPARILWMAVLGTQILATFIAVYGFLMPPLGWKWAGFVWGYAL
                     VWVLVTDRVKLIAYWIFDPVNTKPKSEAKVEPISSATS"
     gene            9519..9926
                     /locus_tag="VAH76_001794"
     CDS             9519..9926
                     /locus_tag="VAH76_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEO1386410.1"
                     /translation="MLNSNILNINPETVCFIIAKAKEFQAKEGVTFPEEAPNSEYEYD
                     MQQILADHADDLSYTEVKQTIEDLGIDEKVDLLSLMYIGRGDFVESDWSSAHKEARNN
                     LPPDLTAHLFSKPTIAYYLEKGLESLGYSCENQ"
     gene            10015..11451
                     /locus_tag="VAH76_001795"
     CDS             10015..11451
                     /locus_tag="VAH76_001795"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013117.1,HMM:NF016836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEO1386411.1"
                     /translation="MRVKKLELRRNKLIPILIGLFWASFITPALGENIKSNVSQSLSS
                     APIADVSNKVDVKPIARDTEISDRIEKILNATTWYENPKVEVTNGVVFLKGKTKTSEH
                     KKWAESLAKNTQDVVAVVNQIEILTSSVWDVSSLVSAGFKEQWQNFLRSIPIIILSFL
                     ILIIFWIIALIVKTISRKYLNSRELHPLLSHVISRGAAFLCVLIGIYFILKLLGLTNI
                     ALTILGGTGVLGIILGIAFKNITENLLASVLLSVQNPFKNDDLIEVAGVTGYVQGLTI
                     RATLLMTQDGHEVQIPNAIVYQSNIYNFTSNPNRRETFLIEIASTASISAAQELALNT
                     LKNHPAILQEPEPLVLVNSLTSGNVVLCIYFWLDGSQYNWLKVKSSAIRLLKRAFQDA
                     EIHIPGTEIKINIANEISLPEKKSKHETVNKPLSINEESQTLVTQAEGELGSDMNDIQ
                     KQAQQSKVITEENNLLTQSKKTPTLKPS"
     gene            complement(11578..11709)
                     /locus_tag="VAH76_001796"
                     /pseudo
     CDS             complement(11578..11709)
                     /locus_tag="VAH76_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(11983..12672)
                     /locus_tag="VAH76_001797"
     CDS             complement(11983..12672)
                     /locus_tag="VAH76_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO1386412.1"
                     /translation="MQHKEYHRIERIGWLRAAVLGANDGIISTASLLIGVAAAHTPYN
                     GIFVAGIAGLIAGAMSMAAGEYISVSSQADTEKSALKREMTELQENLPNEIEELTTIY
                     INRGLQRDFAEEVVKQLMAKDALGTHARDELGITEVTSARPLQAALFSACSFTVGSLL
                     PLLMIFIVPRIYLIPSVSIMAVLFLALLGAVAAKVGGARIVLGSLRVVIWGTIAMLVS
                     AGIGSLLGIGV"
     gene            complement(12723..13208)
                     /locus_tag="VAH76_001798"
     CDS             complement(12723..13208)
                     /locus_tag="VAH76_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO1386413.1"
                     /translation="MRTIGIKIAFISTLLVSGLISPSLFAGSKDAANNKINAVISSKV
                     FEMAKKNENWKLALVTGKDAQVVFMNITPKTNPNNEIGLETHPFDQVIFVVEGQAKSV
                     LNGKKSTVTAGDMIFVPQGIPHNFINLNVKKPFKIISVYSATDIPENAAYKQKSDTPQ
                     E"
     gene            13336..13869
                     /locus_tag="VAH76_001799"
     CDS             13336..13869
                     /locus_tag="VAH76_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEO1386414.1"
                     /translation="MESNKNWHYDTITRLLHWVIALLLIGLISIGWYMMSIEDQPNSG
                     WYFNLHKSFGLIAAMLIFFRLIWRFTHKPAPLPSSVPHWQTIVSRGIHLLLYFCMLIM
                     PITGFIGASFSKHGIIFFGVELPNWVNQSHAISEQFFEVHEVVAWILVGLIALHILAA
                     IKHLVINRDKVFQRMWL"
     gene            complement(13994..14587)
                     /locus_tag="VAH76_001800"
     CDS             complement(13994..14587)
                     /locus_tag="VAH76_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEO1386415.1"
                     /translation="MKLSNEITLKDLFARPVFHAAEAREAGIHPSRLSYYVKTNRIER
                     IGHGVYRGVESTVDADFQWEELIIIVKSIPHGVVCLVSALALYELTDEMPRENWIAVS
                     HSTTAPKREHARVVRMRDMETGKTMYTIGGETVAIFDRERTIIDSFRYLSKEIAIKAL
                     KAAVSSRAPQKLDIKKLQKYAKQFGLNLEPYILAVTT"
     gene            15112..>15794
                     /locus_tag="VAH76_001801"
     CDS             15112..>15794
                     /locus_tag="VAH76_001801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEO1386416.1"
                     /translation="MTGKKRKGSREIHTYLHKGKERLNNPQVGLVTPETDQDGQNTTY
                     LYDPHLDPSLQWAGKNEHTSFEVPTVSLHVHERIDPYSIIRALKRKNKNIQGSLFEQQ
                     ETPLREAIEFYKHKFNWTNRLISGDSLLVMNSLYEKEAMGEQIQMIYIDPPYGIKYKS
                     NFQPFISNNKVAENDRDEDLTGEPETLKAFRDTWELGIHSYLTYLKDRLVLAKNLLTV
                     SGSCFVQIS"
BASE COUNT         4901 a         2987 c         3278 g         4628 t
ORIGIN      
        1 atacaattta tagattatct tatgcattct tgcagcggtc aaaaatgtga accttgcatt
       61 ggggtgagac aatcggatca tcgatttcaa aatacattat gggaacaatt cccgttcaat
      121 atgtacgcac aatcattttt aatgaatgag cacatttgga atgaagcgac aaccgctatc
      181 agaggggtgt cggagcatca tgaaaatctg gtaaatttta ccacccgaca aatactcgac
      241 atgtttgctc catctaattt tcctttcaca aatcctgaag tgattgaagc gactattaac
      301 gagggggggg ctaattttgt taatggatta aataattgga ttgaagatgt ttcgcgtaaa
      361 ctcaataaac aaccacccgt tggttcagaa caatttaagg tcgggaaaaa tattgcgatt
      421 accccgggaa aagtggtcta tcgaaatcat ttaatcgaac tcatacaata tgagcctact
      481 actccaaaag tatacctgga acctattctc atgatacctg cctggattat gaaatattac
      541 attcttgatc tttcccccaa taattcaatg gtcaaatatt tagtagatca aggacacacc
      601 gtattcatga tttcatggcg aaatcctgat gaaaaagata gaaatttgac tatggaagat
      661 tacatcagtc tcggcgcaat ggatgcaatg aacgcgataa cgagtattgt gccgaaacaa
      721 aaaattcatt cagtcggata ttgtcttggt ggcactttat taatgctcgc ggcggcaagc
      781 atagcaaaag aaaaggataa tccattaaag tccatcaccc tgtttgcatc gcaggtcgat
      841 tttaaggatg cgggcgaact catgctcttt atcgatgaga gtcaaatagc ctatcttgaa
      901 gacatcatgt ggacaaaagg ctatttagat gggcaacaaa tggcaggaac atttagtatg
      961 ctgcaatcca ttgatcttat ctggtctaaa attatgcttg attatcaatt gggaaaaaga
     1021 agaccaatca acgatcttat ggcatgggat tatgatacaa cccggctgcc ctatgcgatg
     1081 cattcagaat accttagaaa attattttta aataatgatc ttatagaagg ccattactgt
     1141 gtgaatggga aaattcttac cttgcaggat attgagcttc ctatttttgc agaaagcgcc
     1201 ttaagagatc acgtcgcccc ttggaaatct gtttataaaa ttcattattt tactgataca
     1261 gacatcacct ttgtattaac caatggtggt cataattcag gtgtggtcaa tgaaccaggt
     1321 caccctggac gtagttatca actcatgacc cataaaaaag gcgataaaga tatttttcca
     1381 gaactttggc aacaaaaggc ctcacaaaac gaaggatcct ggtggcctgc ttggcaacaa
     1441 tggctcgcca gttattctgg taaaaaagtg ttgccaccag ctatgggaaa tgcgaaacga
     1501 ggctataaag ttttatgtga tgcaccaggt acctatgtat tgcaaaaata gattgaagct
     1561 taaaaaaagg agtttttatg aaaaatcgta ttgctttaat tactggagga atgggagata
     1621 tcggaacggc catttgccac gaattttgta accgaggagc taaggttatt gctgccgatc
     1681 gtttagataa agacaaagca ttggcatggc aagcccagca aaagaaactc gggtttgaga
     1741 tggggattgc ctccatggac gttactcagt ttgaaagctg tgttgctttg gcaaaagatg
     1801 ttgaacaacg ttttggccct attgatattt tagtcaacgc tgcaggtacg attaatgacg
     1861 ttaccttgcg gaaaatgaca ccagaacaat ggaatcaagt agtccatacc gatatggaca
     1921 gtatgtttaa tgtgacacga caatttatta atggaatggt tgaacgcggc tatggacgta
     1981 ttgttaatat ctcatcggtt aacgcagaaa aaggtcaatt tggtcaaacc aattattcat
     2041 cagcaaaatc aggtgtttat ggatttacca aaagtctggc ccaggaggtt gcgaaacacg
     2101 gagttactgt caatactatt tctccaggtt atgttgaaag tagaatggtc atgtcggttc
     2161 cagaacctgt tcgtttaaaa atcatagaaa atatacctgt aggccgtttg gcaaaacccg
     2221 aagaaattgc gtggtcaatt gcttttttag catcagagcg tagcggattt atgactggct
     2281 ctaacttggc ggtcaatggt ggaattcata tgtattgatg aagaagaatc atcaatacat
     2341 gaaaagggag atatcatgga ccataaaaac gatcctattg ttattgtcag cgctgtacgg
     2401 actccaattg gtcatttcgg tggatatttc aaaaattgtt ccactataga gcttggtgcc
     2461 acagccatta aggctgtcat tgagcgagct aaactcaaac cagctgacat ccaggaagta
     2521 ttgatgggat gtgtattacc cgcaggtctt ggtcaggctc ctgctcgcca agcgtcaata
     2581 ttagctgggg ttccgacttc agttccttgt gtgactgtaa ataaagtgtg tggctcgggc
     2641 atgcaaacag tcatttttgc ttatgatgag ctacaagccc atagccgcaa tattattatt
     2701 gcaggtggga tggagaatat gtcacgagct ccttacttgc ttccaaaagc gcgatttggc
     2761 tatcgactgg ggcatggcga aatccttgat catatgatgt tcgatggttt ggaagatcct
     2821 tattcgaaaa aagccatggg ctttttcgca gaacagtgtg ccactaaata taaattttcg
     2881 cgaaaagaac aggatgatta tgccattgaa tccttccacc gagctaaaca tgcgaccgat
     2941 aagggatact ttgcagctga aatctgtccc gtttcagtga ctgataaaca tcacaacgta
     3001 gtgactgtct ctcaagatga aggaatatcc tgtgtcgatc ctgaaaaaat tccaaagctt
     3061 catccggcat ttgtaaaaga tggaacagtg accgcgggga cttccagtca gatttcagac
     3121 ggagcttctg cactgataat catgcgccaa tcagaagccg aaaaaagagg aatcaaacca
     3181 attgccaaaa ttttagggca ttgtatcttt gcccatgatc ccgcctggtt tactactgct
     3241 cccatcggtg ccattaacgg ggtgcttgaa aatacaggat taaccacaaa ggatattgat
     3301 ttatatgaaa ttaacgaagc gtttgccgtt gtacctatgg ctgccattaa agagttaaaa
     3361 ctctcagaaa aacaggtgaa tttttttggt ggtgcttgtg tactgggcca tccactgggt
     3421 gatacaggag cgagaatcat cgtcacacta ttaaatgcat tgcaccaaaa aaaattacat
     3481 cgtggtattg cagcattgtg tatcggcggt ggagaagcaa ctgccgtcgt agtggaaatg
     3541 atgccttaat ctgtaaataa aagggagatt ttacatgata attgacctga caaacaaaaa
     3601 aggtttagta accggcatcg ccaatgaaca cagcattgcc tacggatgtg cggaacaatt
     3661 ttgtgaagcg aatgcagaat tggcaacaac ctttcttaat gccaaagcag aacccttcat
     3721 gcacccttta gcactagaat taaagtctcc acttattctt ccttgtgatg tgcaaaaaga
     3781 agaagagatt attgctttat ttgctgctat tgaaaaaaaa tgggggaagc ttgacttcat
     3841 gttgcattcg atggcctttg cccctaaaac tgacttggaa gaccaactgg ttgattgtac
     3901 taaggagggg ttcctcgaag cgatggatat ctcctgtcat tctttaattc gactgtcaaa
     3961 atacgccgca cccttgatga aaaatggcgg tagtatcttg actatgacct attatggcag
     4021 ccaaaaagtc atacttaatt ataatttaat gggtgtggtc aaggcggcat tagaggcatc
     4081 ggttagatac ctggcatatg aattaggacc caaaaacatt agagtcaacg ccatttcacc
     4141 aggccccatc caaacacggg ctgcatctgg aattcctaat ttttatgagt acatcgatgc
     4201 agaaaagcag cgctccccgt tgcatcgttt ggttgatatt aaaaatgtcg gtaatttagc
     4261 agcattcctt gccagcgatg cggctatgga tatgaccgga caagtacatt atgttgatgc
     4321 aggttactcc attatgagtt gatgattatc aaggagcatt aagatgtctt ttattgaaaa
     4381 tcgaacattt gatgagctta aaattggtga ctcagcacac atagagcgca cattaactcc
     4441 aaaagatatt gaactctttt ccatcatgtc tggtgatgta aatccagctc atgtggatgc
     4501 tgaatatgca aaaaatgata tgtttcatca aatcattgta cagggtatgt ggggtgctgc
     4561 actgatatca acagtacttg gtacagagct cccaggccct ggaaccatct atttagatca
     4621 aacacttcga ttttccgccc ctgttgtccc cggtgataca atcacgatta gcgtaacagt
     4681 gaccgataaa aaacctgaac accatattgt tgttttagaa tgcaagtgcg tgaatcaaca
     4741 tgataaagtg gtaatcagtg gtgttgccac agtgatggca ccaaccctta aagtgaaaag
     4801 agaacgaatt gaattaccca aagccgtttt aaaggagcct aaaggtacat ggtataaaaa
     4861 gttaatcaaa ttaacaaaag atatgacacc tcttaaaaca gctatagttc atcctgtcga
     4921 tccacttgcc ctacaaggtg ccgttgatgc agcaaatgaa catctcatta tccccattct
     4981 tgttggtcca aaggataaaa ttcttgcggc agcccttgag gccaaactgg atatttcagc
     5041 gtatgagctg attgcaactc gccatagcaa tgaatcagct gaagttgcag taaaacttgt
     5101 aaaagaaggc aaagcagagg cgttgatgaa agggaaaatc cacactgacg aattgatggg
     5161 gccagtggta aataaagaaa cgggcttact cactggacgg agaatgagcc atgtctttta
     5221 tgtcgaagcc ccaaaatatt tcaaaccttt atttctctct gatgccgcac tcaacattcg
     5281 gcctagttta gaagaaaaaa aggacatcac ccaaaatgcg attgatttgt ttcatacctt
     5341 aggctttggc acgccaaaag tcgccattgt ttgcgctgtc gaaacagtca atgaaaaaat
     5401 gcaatccacg cttgatgccg cagcactaag taaaatggca gaccgaggtg aaataactgg
     5461 cgctattgtc gatggccccc tggcatttga taatgccatt tctattgaat ctgcacgtga
     5521 gaaaaatatt aaatcagagg ttgcgggcta tgctgacatc attatcgtac ccgatattga
     5581 atcaggcaat atgctctata aagaaatgac ttatatgtcg ggcatggaag ccgctggcat
     5641 ggtaatgggt gcgagtgtcc ccattatatt aaccagtcgt ggaagtgatg atatttctcg
     5701 taaagcatct tgtgctatgg cgctgatata tgttcgtaat aaggagaaat tacatgactg
     5761 aagtcctatt agtgattaat acaggttctt caagcattaa attttcgatt tttgcctgtc
     5821 aaaaaaaatt aaatttactc tatcatggcg aaattgaaag tatttcagaa tctccatctt
     5881 taaccatttt agacgcgaac catacacaaa tagtagacaa aaaaatttca tctaaaggaa
     5941 ccgaagcggg gttacgagcg ttttttaatt ggtttgagca tttacccgac tccatgaaat
     6001 taaaagcagt aggacatcgt gtagtgcatg gaggcaccta tttttctcat cctacattag
     6061 tgactgatga ggtcgtgaaa aaaataacca gtttaattcc tttttgccct tttcatgagc
     6121 cgcagagtct cgaggccatt aagagcatta aaacgattta ccctgacatg ccgcaagtgg
     6181 tttgtttcga taccaccttt catcgcaccc aagagcattt ggcaacgctt tttgctatcc
     6241 caagaaagtt aacagacgaa ggtctgattc gttatggatt ccatggtatt tcctatgaat
     6301 acattgcctc agtcatcacc caacatattg gcgatatagg caataaacgg gtgattgtgg
     6361 ctcatttagg tagtggtgcg agtatgtgcg ccatgcatca acgtaaaagt gttgccacct
     6421 cgatgggact taccgctttg gatgggttga tgatgggtgc tcgcagtggt tcgattgatc
     6481 ctggattaat attgtattta ttgcaagaaa aaaaattatc agtggaacaa gtaactaccc
     6541 tgctttatca agaatctggt ttattaggcg tctctggcat tagcagcgat atgcgaaaac
     6601 ttcaatcaag cgatgaaccc catgcaatag aggcggttga tttattttgt tttatcgcgg
     6661 cgagagaact aagtgcacta tgtggcatct tacaaggatg cgatgcaatt atttttacgg
     6721 caggtattgg tgaaaaatcg gtactcgtta gaaaaaaaat ctgtgaccga ttaaactggt
     6781 taggtgttgt cttggatgag aaggcaaata gcactaatgc atccgtcatt agccaggact
     6841 cgagcaaaat taaagtaggt gtcatcccaa ccaatgaaga atacatgatt gctaaacata
     6901 caatggatgg aatagttgaa actattaatt agtactgttt caaaaggata aaatgatgaa
     6961 aacaagtacc aataagcacc atgctaaaaa atcgaaacaa aacaccaaga acgacctcaa
     7021 atcgattccc atggcggaat tattgaaaaa attggataca acacagaatg gactcagtca
     7081 agctgaggca aaaaagcgat tgattcaata tggtcctaat gagcttgaag aaaaaaacat
     7141 taatcctttc ttaaaatttc ttacctattt ttggggacct ataccctgga tgattgaagt
     7201 agcggtgatt ttgtctgctg tggttcagca ctggccggac tttttcatca ttttaatcct
     7261 cctcatttcc aatgctgtag tgggattttg ggaagaatat caggctggta atgtcatcgc
     7321 ggctcttaaa gcaaaattag ccattaaatc tttggtgaaa cgagatggga aatggataac
     7381 cccacctgct tgtgaattgg tacctggtga tattatccgg atgcgactgg gcgatattgt
     7441 acccgcagat gcgcgtttgc tagacggcga ctcaattgag gtagatcagt cggcactaac
     7501 aggagaatca ttgcctatca cacgccaatc cggcgaggcc gtattttcag gatctatcat
     7561 tcgtcagggt gaaatcagtg cgctggtcta tgctacgggt caaaaaacct acttcggtaa
     7621 gacggcacaa ctggttgagg aggcacacac ggtcagccat tttcaacgcg cggtacttaa
     7681 aattggtaat tacctcatca tttttgcttt agtgatggta tcggggatta ttagtgtcgc
     7741 gatctatcgt ggcgacccta tccttaccac gttacagttc gctttggtgt tgaccgtagc
     7801 tgctatacct gtggcaatgc caacagtgtt atcggtgacc atggcggttg gtgcgcgctt
     7861 gctggccaag aaaaaagcga ttgtaagcaa gttggtggct attgaggagt tagcgggtgt
     7921 tgatgtgttg tgcgctgata agactggcac tctgactcaa aacaaactga cgctgggcga
     7981 cccattctgt gccaaaaagt tcaccgctga acaagtgatt ctcaatggtg cattggcttc
     8041 acgagtggac gataacgaca ccattgatat ggctgtcatg gctggattga aaagcaaaga
     8101 ttcgttaaag ggttataaaa tgcttcattt cataccattt gacccagtac acaaacgcac
     8161 agaggccacg attaagggcc cagacggaaa ggagtttaag gtaaccaagg gcgcaccgca
     8221 ggtgatcttg aaattagcag ccaatgccgc tcagataaaa gctgatgcta ccaaggcggt
     8281 cgatgagttc gcagcacgtg gctttcgttc gttgggtgtg gcacgcgctg agggagaggg
     8341 taaatggcag tttgttggtg tgctaccttt gtttgatccg ccgcgcaaag atgccaagaa
     8401 aactattgcg actgcttttg caatgggcgt aaagatcaaa atggttactg gcgatgcttt
     8461 agcaatcgca aaggaaactg cagagaaact ggggatgggc tcaaatattc ttgatgccag
     8521 tactttgggt gatgcaaatc gaaaagaaac gacaaagatg accaagtaca tcgaaaatgc
     8581 agatggtttc gcccaagtgt ttcccgagca gaaatttcac atcgtagatg ccttgcaaaa
     8641 aagcggacat atggttggca tgacaggcga tggagtgaac gatgcccctg cactaaaaaa
     8701 agcggattgc ggcatcgcgg tgtcgagtgc catggatgca gcacgtgcgg cagcgtccat
     8761 cgtattaaca acacctggcc tatcagtgat tattgatgca atcaaagaaa gccggaaaat
     8821 tttccagcgc atgaatagct atgcgattta ccgtatcgcc gagaccttgc gtgtcctgtt
     8881 gtttatgaca gcagccatcc ttatcttcaa tttttatccc ttgacggcag tgatgatcgt
     8941 gatgctggca ttactcaatg acggggccat cctatccatt gcctatgata acgttcatta
     9001 caaggatgcg ccggaggcat ggaacatgcg catggtgcta gggatcgcct cagttttggg
     9061 catcattggc cccatagctg cttttggcct attctacctt ggcgaccaag tgtttcacct
     9121 cactcacaag cacctccaac caatgatgta tttgatgctg tcagtggccg gtcatatgac
     9181 catctttatt actcgcacgc gtggcccatt ctggtccata cgcccggcac gtatattatg
     9241 gatggcggtg ttggggacac aaatcctggc gacgtttatt gcggtttatg gcttcctgat
     9301 gccgcctctc ggctggaagt gggcaggatt cgtatggggg tatgcgcttg tttgggtact
     9361 cgtaactgac cgtgtgaagc tcatcgcata ttggattttt gatcccgtta acacaaaacc
     9421 taaatcagaa gccaaagtag agcctatatc ctctgcaact agttagatca aagcaaaagt
     9481 ttgtttcttg ctttgttaat gagggaaagg agataaacat gttaaattca aatatattaa
     9541 atattaatcc tgagactgtt tgttttataa ttgctaaagc aaaggagttt caggcaaaag
     9601 agggtgttac ttttccggaa gaagcgccga actcagaata tgagtatgat atgcaacaaa
     9661 ttttggcaga ccatgctgat gaccttagtt atactgaagt aaaacaaaca atagaagacc
     9721 taggtattga tgagaaagtt gatctccttt ctttgatgta tatagggaga ggtgattttg
     9781 ttgaaagcga ttggagtagt gcacataagg aagcaagaaa taatttaccc ccagatttaa
     9841 cagcccattt attctccaaa cccactattg cttattattt ggagaaagga ctggagtctt
     9901 taggatattc atgtgagaac caataagtaa atattttaac ttacagggcg ttggcaaaat
     9961 agttgcagga aaaacggacc ataaaacgga acctatttaa ggatattacc tctcatgcga
    10021 gtaaaaaaat tagagctcag aagaaataaa ttaataccta ttttgattgg tttgttttgg
    10081 gcaagtttta ttactcctgc tttgggagaa aatataaaat caaatgtatc gcaatctctt
    10141 tcctcagcgc caatagctga tgtttctaat aaagtagatg taaagcctat cgctcgagat
    10201 acggaaataa gcgatcgaat tgaaaagata cttaatgcaa ctacttggta tgaaaatcca
    10261 aaggtagaag taacaaatgg ggttgtgttt ttaaaaggaa aaactaaaac gagtgaacat
    10321 aaaaaatggg ctgaatcctt agcaaaaaat acccaggacg tcgtggctgt tgttaatcaa
    10381 atagagattc ttacttcatc agtatgggac gtgagctcat tagtaagtgc tggctttaaa
    10441 gagcagtggc aaaattttct aagaagtata ccaataatta ttttatcctt tttaatcctc
    10501 ataattttct ggattattgc gttaatagta aaaactataa gtcgcaaata tttaaattct
    10561 cgagaacttc accccctttt atcccatgta atatctcgcg gggcagcttt cttatgtgta
    10621 cttattggaa tttatttcat tttgaaactt ttaggattaa caaatattgc gctgactatt
    10681 ctgggaggaa cgggtgtact gggtattatt ttgggtatcg cttttaaaaa tattacagag
    10741 aatcttttag caagtgtctt actaagcgtt caaaatccat tcaaaaatga tgatttgata
    10801 gaagttgctg gagttacagg gtatgtacag gggttaacta ttagagcgac tttattgatg
    10861 actcaagatg gacatgaggt gcaaattcct aatgctattg tataccaaag taatatatat
    10921 aattttacaa gtaatccaaa tcgcagagaa acatttttaa ttgaaattgc cagcacagcg
    10981 tctatttctg cggctcaaga actggcttta aatacactta aaaatcatcc tgctattttg
    11041 caagagcctg aacccttagt tttagttaat agtttaacat ctggtaatgt tgttttatgc
    11101 atttattttt ggttggatgg aagccaatat aattggctaa aagttaaatc atcggcaatt
    11161 cgtttgctaa agcgcgcatt tcaagatgca gaaattcata ttccaggaac tgaaataaaa
    11221 ataaacattg ctaatgaaat ttctcttcca gaaaaaaagt ccaaacatga aacagtaaat
    11281 aagccactat ctataaatga ggagtcacaa accttagtca cgcaagcaga aggagaacta
    11341 ggcagcgata tgaatgacat tcagaagcag gcacaacaat caaaggttat tactgaggaa
    11401 aacaatcttt taacgcaatc aaaaaaaaca ccaacgctta aacctagtta acttaactac
    11461 gtcacttaat gataaagtgc gaaactagat aaaattagtc aaaacatcgc cgtccatagc
    11521 ttgtttaaaa cctggaattt ctgattgata agctatcttc tccaggaaaa taagatttta
    11581 ctttgccaaa gaaaatggcg tttgaatcaa atttctgata acagcaataa cttctgttaa
    11641 tgatttctgc tctaattcat tctttctcaa aaaagctttc cacattgatt ctctagttgg
    11701 atcattggct tctgatttat caatagaaca tccagcatgg atatagacta taaattcatc
    11761 tttcgtatcc agtcctaatc ttactaattc gagtttatta gacatgttat ccaatactaa
    11821 ttcctttgtc tgttgtacta aagattcagt ttgtaaatat tcaggtatga tgcaagacca
    11881 attaaattgt tccattatga tttttattct caaactgaag cataggtaat gctctttttc
    11941 ttgctttacg attagaccgt tggcatacct ggtagtcgca ttttatacgc ctatgcctaa
    12001 cagcgagcca atacccgcac ttacaagcat cgctattgtt ccccatatca caacacgcaa
    12061 tgatcctaat actattcgag ctccgccaac ctttgcagcc actgctccga gtaatgccaa
    12121 aaatagaacc gccattattg atactgatgg aataaggtag attctaggaa caataaaaat
    12181 cattaataac ggtaataacg agccaacagt aaaactgcag gcagaaaaca gagctgcctg
    12241 aagtgggcgc gcactggtta cttcagtaat accaagctca tcgcgagcat gtgtgcccaa
    12301 tgcatcttta gccatcaatt gctttacaac ttcttcagca aaatcacgct gcaatccacg
    12361 attaatatat atagtagtta attcttctat ttcattcggt aaattttctt gaagttctgt
    12421 catttcacgt tttaaggcgg atttttcggt atctgcttga gagcttactg atatatattc
    12481 acctgcagcc atagacatgg cgccagcaat taatcctgca atccctgcta caaatattcc
    12541 attatagggg gtatgagcag cagcaacacc aatcaataag ctcgcggtag agataatacc
    12601 gtcatttgca cccaatactg cagcacgtaa ccagccaatt cgttcaattc gatgatattc
    12661 tttatgttgc atctatatca gaactcctat actgaataaa tctgcgttaa tttgttacag
    12721 agtcactctt gtggtgtatc agatttctgt ttatatgccg cattttcagg gatatcagta
    12781 gccgaataaa cgctaattat tttgaatggt ttctttacat tgagattaat aaaattatga
    12841 ggaatccctt gagggacaaa aatcatatca cctgctgtaa cagttgattt tttgccattg
    12901 aggacagatt tagcctgacc ttcaacaaca aatatgactt gatcaaatgg atgtgtttcg
    12961 agcccaatct cattgttggg gttggtctta ggtgtgatat tcatgaaaac aacctgcgca
    13021 tctttacctg tcaccagtgc taatttccag ttctcattct tttttgccat ttcaaaaact
    13081 ttggaggaaa tgacagcatt tattttattg ttagctgcat ctttagaacc tgcgaataac
    13141 gatggtgaga ttaaacctga tactaataat gtagagataa aggctatttt tattccgatt
    13201 gttctcataa atgctctcca taatttaaat tagcttcttt caatatatca acttatgagc
    13261 ttgtttaata ccgatagata acttaaccta gaaaaatggc taaggattaa ataaaccggt
    13321 tggataggag ctgtcatgga gagcaataaa aattggcatt atgacactat cactagattg
    13381 cttcattggg ttatagccct tcttttgata gggttaatca gtataggttg gtatatgatg
    13441 tccatagagg atcagcctaa tagtggttgg tattttaacc ttcataaatc ctttggcctt
    13501 atcgctgcta tgctgatttt ttttagattg atatggagat ttacccataa acccgcccca
    13561 cttccatcaa gtgttccaca ttggcaaact attgtatcaa gaggaattca tttgttgctt
    13621 tatttttgca tgctcataat gccaattact ggctttatcg gagcttcttt tagcaaacat
    13681 ggtattattt tttttggagt agaattacca aattgggtta atcaaagtca tgctatatca
    13741 gaacagtttt tcgaagtgca tgaggtggtt gcatggattt tagttggatt aatcgcactt
    13801 catattcttg ctgcaattaa acatcttgtt attaacagag acaaagtttt tcaacgtatg
    13861 tggttataag aatacaacat atacagttta aggaataaga ggaagatatt gattctttga
    13921 atagagtgtg ttgaaaaaat attggcgatt aaagttgctt aataagttca ttgccatgta
    13981 tggaccccgc cattcatgtt gttaccgcca aaatataagg ttcaagatta agcccaaatt
    14041 gtttcgcata cttctgaagt tttttaatat ccaatttttg gggagctcta ctactaactg
    14101 ctgctttaag agcttttatg gctatctcct tactgagata acgaaaagag tctattatcg
    14161 ttctttctct gtcaaaaatg gcgaccgttt cccctccaat tgtgtacatt gttttacctg
    14221 tttccatatc acgcattcta acaactcggg catgctctcg cttaggtgct gttgtagaat
    14281 gagaaacggc tatccaattc tctcttggca tttcatccgt cagttcatag agagccaaag
    14341 ctgagactag gcatactacg ccatgtggaa tacttttgac gatgataatc aattcttccc
    14401 attgaaaatc agcatcaaca gtcgattcaa caccgcgata aacaccgtga ccaatgcgtt
    14461 caattcgatt agtttttaca taataactga gccggctggg gtggatacct gcttcacgtg
    14521 cctctgcggc gtgaaagact ggtcttgcaa acagatcttt caatgtaatt tcattactta
    14581 gtttcatacc cgtaatttta atattaaaac ccaataacgt caaatgggtt tcaaaattaa
    14641 aataatgccg taaaaatcca ttgtggggaa tttgcgtatt tgagtacgac tctgttatac
    14701 ttcgtacgac aattgtagtg gtttcctgcg attaaaatta taataaaatc aatgacttat
    14761 tttagcattg acgggctcat aatccgttgg tcctaggttc aagtcctagt gggcccacca
    14821 ataaaatcaa gtagttacgg tagttttcag gaaattcgaa aaagtcatgt agacgtaatg
    14881 tagacagtta aaagtgctcc ccttatttgg ggtagagcct tatatattta tattgccgta
    14941 cttggcattg tctacacatt gaatgtctca tgataataga ccatactttt agataccatt
    15001 ctaatatcaa gttgtttgag tttagtttta tagatttttt gccaatatac agaattttaa
    15061 attagaaatt tttaaagatt taaaaattat tactaattaa aaggctaatc tatgacaggg
    15121 aaaaaaagga aaggtagtcg tgaaattcac acctatctac ataaaggaaa agaaaggctg
    15181 aacaatcctc aagtaggatt agtcactccc gagacagacc aagacggaca aaatacaact
    15241 tatctatatg atccccacct tgacccaagc ttacaatggg caggaaagaa tgagcatacc
    15301 tcatttgaag tacctactgt atcattacat gtacatgaaa gaatcgatcc ttattcaatt
    15361 attagagcat taaaaagaaa aaacaaaaat attcaaggct ccctatttga acagcaggaa
    15421 acaccgttac gcgaggcaat tgaattctat aaacataaat ttaattggac aaatcgtttg
    15481 atctcaggtg attcattact agttatgaat tctttatatg aaaaagaagc catgggcgaa
    15541 caaatacaaa tgatctatat tgacccgcct tatggcatta aatataaatc taattttcag
    15601 ccatttataa gcaataataa ggtggctgaa aatgatagag atgaagattt aacaggagaa
    15661 ccagaaacgc ttaaagcctt tcgcgataca tgggaattgg gtattcattc atacttaaca
    15721 tatttgaaag atcgtcttgt attagcaaaa aatttattga ctgtttcagg tagttgcttt
    15781 gttcagatct ctga
//
LOCUS       DATNSN010000073        15682 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.073, whole genome shotgun sequence.
ACCESSION   DATNSN010000073 DATNSN010000000
VERSION     DATNSN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.073"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..419)
                     /locus_tag="VAH76_001802"
     CDS             complement(<1..419)
                     /locus_tag="VAH76_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEO1386417.1"
                     /translation="MNSSTSGKNNQQKNPLPTYFSDPPLQHVDLFKLSSYPVLDVEEA
                     IKFTLTEENLAEMLHTMLNHSLPDEVAEMKKFHDLGDWEKTQDIAHKIKGGCVYVGTV
                     QMKMACQYFERYWKSGETDLLEALYQQILHTIEENVR"
     gene            complement(513..3671)
                     /locus_tag="VAH76_001803"
     CDS             complement(513..3671)
                     /locus_tag="VAH76_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEO1386418.1"
                     /translation="MKIALFFIDRPVFATVISIIIVMVGVLSYFNLPIEQYPQVVPPT
                     IQVQASYPGANAKTVAETVATPIEQEVNGVENMLYMDSQSTDDGQMRLTITFKLGTDL
                     DQAQVLVQNRVAVVEPKLPEEVRRLGITTNKNSPDLMLVVNLYSPNGQYDQTYIGNYA
                     ILHIRDKIRRIEGVGNVLIFGASEYAMRLWLNPDLMNTFNLTANDVLAAVRAQNVQVA
                     SGKLNLQPQKKQYGFEYHIETKGRLVKKEEFENIIVKSGEGGRIVRLKDIGRVELGTQ
                     NYLTKGYLDKYPAVALPIYQRPGTNALATAQEIIKTMKTIAKDFPPGITYEIAYNPTL
                     FVQQSIDAVFHTIYEAIALVVLVILIFLQTWRASVIPVVAIPVSLIGTFAVMQAIGFS
                     LNYLTLFGLVLAIGIVVDDAIVVVENMERNIQAGMNPRDAARKTMTEVGSALVAMGLV
                     LIAVFLPTVFLEGISGRFYQQFGTTLAVATAISVFVSLTLTPTMAVLLLRAHHETIKQ
                     EGLAWWRKPVYGFLHGFNRLMEGFSRQYGKLVATLTRKTALMIIAYGIFISITLLLFV
                     YVPRGFIPRQDQGYFIVAVQLPPGASLSRTDAVINKAVKKILAIPGIAHTVSFTGFSG
                     ATFTNSSNAGAIFTPLLSFEERQRMGINYNDILKRLRQELSTIKEALVVVIPPPPVRG
                     IGNAGGFKMMLQDRGGRGLDVLMEAAATMVNAANQEKATTSVFTFFENSTPRLHLKLD
                     REKVERLNVPYANVVEALEVYLGSVFINEFNYLGRTFRVIAQADSEYRHTEDDILRIK
                     VKSNNGDMVPIGSVAQIENTVAPSRMPRFNLYPAIDLQGDVAPGYSSDEALATMEKLA
                     QNNLPDGIGYEWTEIAYQQKMVGNTALMAFTLGVIFIFLVLAAQYESWSLPLAVILIV
                     PMCLFSSMLGVKILGMENNIMTQIGFLVLIGLASKNAILIVEFARQLENRGYNLWKAA
                     IQAAKLRLRPILMTSFAFILGVFPLIIATGAGAEMRRALGVAVFSGMLGVTFFGLIFT
                     PLFYVLISRLSRYKRKLTRPEK"
     gene            complement(3771..4877)
                     /locus_tag="VAH76_001804"
     CDS             complement(3771..4877)
                     /locus_tag="VAH76_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1386419.1"
                     /translation="MKKILALVMRLLAVILIPVLMSCNSESNKPVGQTLAEVDVALPV
                     KKKIVEWDEYTGRFQAVEEVDVRARVTGYLEAIKFQDGQTVKKGDVLFVIDQRPFKYA
                     LARAEAQYALAKAQYERAIKLQKEKFISAEVIDQRFQDVQVAETRVQEAKLNLEFTEI
                     KAPISGKISRDFVSVGNLIRMNDTVLTKVVSVDPIYFYFETSQNDLLKYIRLDRAGKR
                     LSSEKKPTPVLIKLPDEKDYLHQGEVDFLDNVIDSGTGTILARALVPNPDNVIYPGLF
                     ARVKLVGSGEYEAILLPDKAINTDQARKFVYVVDNENKVKRVYVELGPLRETGFYIIR
                     SGLKGDEKVIIHGIQRIHGPNQEVKPVVIEVTEN"
     gene            5094..6932
                     /gene="legS2"
                     /locus_tag="VAH76_001805"
     CDS             5094..6932
                     /gene="legS2"
                     /locus_tag="VAH76_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector sphingosine 1-phosphate
                     lyase LegS2"
                     /protein_id="HEO1386420.1"
                     /translation="MFVRICAMFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYF
                     LYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREK
                     LRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGRGDG
                     KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKE
                     GYGLLTHGGTTSIIEAMAAYVIRARAKGIDYPEIVVPETAHAAFKKAAELTGAILITV
                     PVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDA
                     CLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAA
                     LNWSGGLYATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTT
                     LVEEGAGLTSEDIYVYGNPQWSILGFRSNTCNAHFIADELEKRGWKLNLLQNPDGFHL
                     CLTHVHTVVKGFETQFINDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEIC
                     KQYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQKVQTSL"
     gene            complement(7068..7829)
                     /locus_tag="VAH76_001806"
     CDS             complement(7068..7829)
                     /locus_tag="VAH76_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEO1386421.1"
                     /translation="MLKLFDAHFHIIDYRFPLVANQSYLPPEFTVEDYLQRAKPLNIC
                     GGAVVSGSFQAFDQTYLLTALSVLGPNFVGVTQLPATVSDEEIIQLNQQGIRAVRFNL
                     KRGGSENIHQLKYFAHRIYEMVRWHVEIYVDSKELGDLTNLLLELPAVSIDHLGLSQS
                     GFSELLHLVEHGIKIKASGFGRVDFDVKKALQTIAQINPDALLFGTDLPSTRAPRPFL
                     DSDIQLIRDLFDEELTEKILYRNACNFYSKNKPLA"
     gene            8006..8078
                     /locus_tag="VAH76_001807"
     tRNA            8006..8078
                     /locus_tag="VAH76_001807"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8039..8041,aa:Lys,seq:ctt)
     gene            8196..9299
                     /locus_tag="VAH76_001808"
     CDS             8196..9299
                     /locus_tag="VAH76_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEO1386422.1"
                     /translation="MHQELLKYLEMYHPQIAPFMHLQINEWGKNRPLEGVKILHHVPL
                     VPNTLLKIAALVAANAEVTVTNPASFCQAHPDAITCLHKSGIPYVENLDLLRDETFDV
                     YLDCGGQLYQSLGRPLRGAIELTGSGDQYYRSLKTLDFPVISIDHTMTKQLETVFGCA
                     ESSHQAIQKLTGVDPTNKNWLIFGFGKIGRGLAYFCVEHQVPVTVVDSSVHQCDLASN
                     LGIESIRPEEHDKLEKAVAKADIILTATGGKNIMSKYPRAWFDGKILGNLGLHDEFGP
                     NFTSQDVLYDKKPINFCLYDPTPMIFIDPEFYLHNLGCLLLLQGNFPRGMHDIPEDID
                     SSIIKDWCQYHRRSEKSIRRWFLTSQDFSLKTN"
     gene            complement(9422..10432)
                     /locus_tag="VAH76_001809"
     CDS             complement(9422..10432)
                     /locus_tag="VAH76_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386423.1"
                     /translation="MSTDNLLQPKLIEATLSNYPVIQNMARFYVYDMSRYCGFISEEW
                     ACPPDGLYESYDFKIYFTDPERKAFLIQIENELAGFALLNKFGTQAVDWNMGEFFILA
                     KFQGKGIGQSVAHQLWRTHPGEWEISVIPENIPALSFWRQAVMTYTLNQYKEQIKTVN
                     YDIRQTQRIILSFNTQQQNKSCHSIPPKPITIDFVDSIPESLEKRMTEDLIAYERTHG
                     IDVNYKRFSIVLSAEGTPCGVINAFTAFAEIYIDDIWVDKTYRGKGYGRKLLQTLEDH
                     FKGQGFNNINLCTSAFQAPEFYKKCGYTAEFTRINQANPKLSKTFFVKFFDEENQTQG
                     IF"
     gene            complement(10635..11249)
                     /locus_tag="VAH76_001810"
     CDS             complement(10635..11249)
                     /locus_tag="VAH76_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386424.1"
                     /translation="MVWDATTTNAISNAVHAFFLLLYLIGACIHYFKKDHTFSLLIVF
                     FFLNLLVLKVLGVYVHYYPSHLHLPPAWIAISLLVIMLNYLLVQSIQMPDLCRVIVVF
                     LSIIFTYLFLTHDGNYTYIALPVVLVCLIAAYYSQAKVRIGFVMVVISNLIWIVTRHI
                     ENYLTGHEIPLEYRYDNDIYHILLILSTYVIYRGIAEGQWRHSH"
     gene            complement(11406..11780)
                     /locus_tag="VAH76_001811"
     CDS             complement(11406..11780)
                     /locus_tag="VAH76_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1386425.1"
                     /translation="MLSDPNLVLFYVKNPAKSEEFYKNLLDTQPIESSPTFAMFVMKT
                     GLRLGLWAKEEIEPKAHQTGGGMELSFQVSSNEMVDEIYKQWSDKEISILQPPTQMDF
                     GYTFVGVDPDEHRLRIFCLKRT"
     gene            11971..12744
                     /locus_tag="VAH76_001812"
     CDS             11971..12744
                     /locus_tag="VAH76_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386426.1"
                     /translation="MNSRIETFHQMERHFFSLISVELLLLEGLQACNSGMPIPALNPV
                     FYSGKWTLLRPEIYQCKRFYESHSTPWALVVPDYLSDTEADKVLAQSGFLSIDQGVAM
                     YLPIGDLGISELVSDLLIRRMDNEIDTWILPTIPAFESTEEIAKIYRVRHQEAIEKSP
                     GISHFSGFIQEQIVCSLTLTVMGNSARIDDVATFPKHQKKGYATQLILSVLQTLKDTN
                     ISWCFLEASADGLNIYKKIGFRELFKNLYYEEQCQYEQN"
     gene            12731..13417
                     /locus_tag="VAH76_001813"
     CDS             12731..13417
                     /locus_tag="VAH76_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEO1386427.1"
                     /translation="MSKTERLFHLLQILRRYRYPLSGQFLANELDVSIRTIYRDIAVL
                     QAQGADIEGEPGLGYVLKPGFMLPPLMFNEEEIEALVLGSRWVAKKTDTHLQQAAHNA
                     LAKIASVLPADLRQQLELSSLLIGPGEVISGEDESLALVRKAIRKELKLFIQYHDLKG
                     NVSTRTIWPIGLGYFDQVRVLIAWCELKKDFRHFRADRIVDVENLGVSYAKRRSVLLH
                     DWRKQNNIPG"
     gene            complement(13577..14836)
                     /gene="lem19"
                     /locus_tag="VAH76_001814"
     CDS             complement(13577..14836)
                     /gene="lem19"
                     /locus_tag="VAH76_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem19"
                     /protein_id="HEO1386428.1"
                     /translation="MIDMISITTYWQQFKDCIDQLRSSVSNKEFSNQIAVADLMIEIG
                     LTIEQLGITQIQQVGDRLHEKAKLFGYRVPFVTPEKKYEFCLPKITNAETLIFAIVYY
                     QHLKNYENVEYQGSINWFRSKVIHGVNEKKISTRAQAEAMLGEIRRYCQSQNIAFNES
                     EFIKKNDESAKEYTTRLFKNFHADKTAKSEPIGLKQLNESKESNLSDELAKLEKSLED
                     SRNKRNQLIVKIDALAKKLADYHLLSQNHHKLNDEWNNKWFFTKFFDWIMSWFSNDSF
                     IKNLKTSYEQVINAEHELKQEFHPFDTTDAYRAELKKQLEETHIECDKNQKQINEYQL
                     KQLEHKLEQRLEQKLQEKLQKDTAVEQKEPSVTKVLSEIKHETNGSDFSSNLNHYYGF
                     FKEHLPNRETLGAIAVGVAAIAIQNLM"
     gene            complement(14971..15483)
                     /locus_tag="VAH76_001815"
     CDS             complement(14971..15483)
                     /locus_tag="VAH76_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386429.1"
                     /translation="MSVINGKKIITIILGIACLFCSIHPVNSAPDFSNKPHVREKNKN
                     LLGVFVGGNHAQNDEAFTYGFEYHRVLSMPFGFSAVIEHTPVNVEHNNQVELIGLGTL
                     NVFRNLTFGIGPGIRYEKDEPNRMVGRLGIGYIIHFPPDIEITPNIDLDFIEGGEKEL
                     IFGITFGKQF"
BASE COUNT         4679 a         2953 c         3111 g         4939 t
ORIGIN      
        1 taacgtacat tttcttcaat tgtatggaga atttgttgat ataatgcttc tagtaagtca
       61 gtctctcctg acttccaata gcgttcaaaa tattgacaag ccattttcat ttgcactgta
      121 cccacataaa cgcatccgcc tttaatttta tgtgctatgt cttgggtttt ttcccaatct
      181 cccaagtcat gaaatttttt catttcagcg acttcatccg gcaacgagtg attgagcatc
      241 gtatgaagca tctcggcaag attttcttca gttaatgtga atttaatggc ttcctcgaca
      301 tcaaggaccg ggtaagatga aagcttgaac aagtctacat gttgaagagg tggatctgaa
      361 aaataagtgg gtaaaggatt cttttgttga ttattttttc cagaagttga agaattcata
      421 tgtcctcata agaataattt ctgaagaatg cagtctatat ttttaaacta tagactattt
      481 ataaataact tagggactcc tgcaagtcga tttcatttct caggcctggt caatttccgc
      541 ttatatctac tcaagcgact aattaaaaca taaaacaaag gcgtaaagat aaggccaaaa
      601 aaagtcactc ccaacatccc actgaataca gcgacaccga gcgctcggcg catttcagcg
      661 cctgcccctg ttgcgataat caagggaaaa acaccaagaa taaatgcaaa tgaggtcatc
      721 aaaatcgggc gcagacgaag ttttgctgcc tgaatggcgg ctttccagag attatagccc
      781 cgattttcaa gttgtctcgc aaattccaca atgaggatgg catttttcga tgcaagtcca
      841 atcaaaacca gaaaaccaat ctgagtcata atattatttt ccatcccgag aattttaact
      901 cccagcatgg aagaaaagag acacatcggt acaattaata tgaccgctaa gggtaaagac
      961 cagctttcat attgagcggc aaggacaagg aaaatgaaaa taacacccag agtaaaagcc
     1021 atcaatgcag tattacctac cattttctgt tgataggcta tttctgtcca ctcataacca
     1081 atcccgtctg gaagattgtt ctgagccaat ttttccatgg tcgccaacgc ttcatcagaa
     1141 ctgtatccgg gagcaacatc accctgcaaa tcaatagcgg gatacagatt gaaacgaggc
     1201 atacgtgagg gagcaaccgt attttctatt tgcgcaaccg agcctatagg aaccatgtct
     1261 ccgttgttgc ttttgacttt gattcgcaaa atatcatctt cagtatgccg atattctgaa
     1321 tcagcttgcg caataacacg aaaagtacgt cccaggtaat tgaattcgtt aataaaaaca
     1381 gagccaaggt aaacttccag tgcttccacg acattggcgt atggcacgtt aagtcgttcc
     1441 accttttctc tgtcaagttt caagtgaagt cgtggtgttg aattttcaaa aaaagtaaat
     1501 actgatgtgg tggctttttc ctgattcgct gcattaacca ttgttgctgc cgcctccatc
     1561 aatacatcaa gacctcgccc acccctgtct tgaagcatca ttttaaaacc acccgcattc
     1621 ccaatacctc ggactggggg aggagggata accacgacaa gggcttcttt gatggtactt
     1681 aattcctgcc ttagacgctt aagaatgtcg ttataattta tccccatacg ttgtctctct
     1741 tcaaatgaca ataggggggt aaaaatggca ccggcattcg atgaattagt aaatgtagct
     1801 ccagaaaagc cggtaaaact aacagtatgg gcaatgccag gaatcgccaa tattttcttg
     1861 actgctttgt tgatgacagc atcggtacgg ctcaaagaag ctccaggcgg caattgaact
     1921 gcaacgataa aatagccttg gtcttgtctt gggataaaac cgcgcggcac gtaaacaaat
     1981 agaaggagtg tgatggaaat aaaaatacca taagcaataa tcattaaagc tgttttacgt
     2041 gtgagggtag caaccaattt cccatattgc cgggaaaatc cttccattag ccggttaaat
     2101 ccatgcaaaa atccatatac tggttttctc caccaggcta acccttcttg ttttatagtt
     2161 tcatgatgag cgcgaagcaa taataccgcc atggttggag tcagggttaa cgagacaaaa
     2221 acagaaatgg cagttgcgac agcaagcgtt gtaccgaatt gctgataaaa acgtccggaa
     2281 attccttcca gaaaaacggt aggtaaaaat acggcaatca acaccaaacc catagcgacc
     2341 aatgctgatc ctacttctgt catggttttt ctggcggcat cgcggggatt catgcctgcc
     2401 tggatattac gctccatatt ttcgacaacg acaatggcat catcaaccac aataccgata
     2461 gcaagaacca gtccaaacaa ggtcaagtaa ttcaacgaaa atccaatagc ctgcattacg
     2521 gcaaaagttc ctatcaatga taccggaata gcaactactg gaatgacaga agccctccag
     2581 gtttgtagaa aaatcaaaat gacgagtaca accagggcaa tcgcttcata aatggtatga
     2641 aataccgcat caatcgattg ttgaacaaat agagtaggat tataggcgat ttcataggta
     2701 atcccgggtg gaaaatcctt ggcaatcgtt ttcatggttt taatgatttc ttgcgcggtg
     2761 gctaatgcat tggttcctgg gcgttggtaa ataggtaatg caacagcagg gtatttgtct
     2821 aaataacctt tcgtcaggta attttgcgta cctaactcga cgcgaccaat gtctttcaag
     2881 cgaacgatac gcccgccttc acctgatttg acaataatat tctcaaattc ttctttttta
     2941 accaggcgtc cctttgtttc aatatggtac tcgaaaccgt attgtttttt ctggggttgc
     3001 aaattaagct taccgcttgc gacttgcacg ttttgagcac gaacagctgc taaaacatca
     3061 tttgcggtta aattaaaagt attcattaaa tcgggattga gccaaagacg catggcatat
     3121 tcactggccc caaatatgag tacattgcct acaccttcaa ttcgtctgat tttatcgcgt
     3181 atatgcaaga tggcgtagtt gccgatgtaa gtttgatcat attggccatt aggggagtac
     3241 agattcacta ccaacatcaa atcaggtgaa tttttgttag tagtgatacc taagcggcgg
     3301 acttcttccg gtaatttggg ttccacaact gccaccctgt tttgcaccag tacctgcgct
     3361 tgatccagat cggttccaag cttaaaagta atagttagac gcatttgtcc atcatcagtg
     3421 gattgggaat ccatatacag catgttttcc accccgttaa cttcctgttc aatgggggtt
     3481 gccacggttt cagctactgt ttttgcatta gctccaggat aagaagcttg tacctgaatc
     3541 gtagggggaa ccacttgcgg gtattgttct attggtaaat tgaaatagga caataccccc
     3601 accataacaa tgatgatgga gattactgtt gcgaatacag gcctatcaat aaagaaaaga
     3661 gctattttca tccagtaaat ccttttcttg ttggctgcac tgtaaaacgt tgtatgcttt
     3721 atgcagtatt aaattctatc gaatgaacag gatttcaaca cacaactggt ttaattttca
     3781 gtcacctcaa ttaccacagg cttaacttcc tggtttgggc catgaatacg ttggattcca
     3841 tgaatgataa ctttttcatc ccctttcaat ccacttcgaa taatgtaaaa cccggtttcg
     3901 cgcagtggcc ctaactcaac atacactcgt ttcactttat tttcattatc aaccacatac
     3961 acaaatttgc gggcttgatc tgtattgatt gctttatcgg gaagcaaaat tgcttcatat
     4021 tccccacttc caactaattt tacacgtgca aaaaggccag gataaatgac gttatcagga
     4081 ttaggtacaa gtgctctggc aagaatagtt cctgtgcctg agtcaatcac attatctaaa
     4141 aaatctactt ctccctgatg caggtaatcc ttttcatcag gtaacttaat taagactgga
     4201 gttggttttt tctccgaact gagccttttt ccagcgcgat caagacgtat gtatttgagc
     4261 aaatcatttt gactggtttc aaaataaaaa taaatgggat caacagaaac tactttagtc
     4321 aagactgtgt cattcatgcg gattaaatta cccacactca caaaatcgcg acttattttg
     4381 ccactgatgg gggctttgat ttcggtaaat tcgagattca attttgcttc ctgcactctg
     4441 gtttcagcaa cttgtacgtc ttgaaatcgt tggtctatca cctcagcgga aataaatttc
     4501 tctttttgta gttttatggc acgttcatat tgcgctttgg ctaatgcgta ttgagcttct
     4561 gctcttgcca gagcatattt gaaggggcgt tgatcaataa cgaataaaac gtctcctttt
     4621 tttaccgttt ggccatcctg gaatttgatc gcctcaagat atccagtgac acgggcacga
     4681 acatccactt cttctactgc ttgaaatcgt ccggtgtatt catcccattc tacaattttc
     4741 ttttttactg gaagcgcgac gtcaacctct gccaaggttt gccccactgg cttattggat
     4801 tctgaattac aagacatgag gacaggaata agaataacag ccagtaatcg catgaccaat
     4861 gccagtattt ttttcattgt tatctatccc tgatattcaa gtcactggta agttatgatc
     4921 cagtagataa gcttagttta gcttataaag atgtattatg cgtaataccg tgaatattat
     4981 atcttgcatt tggagatcat ccgggtcaag caaaatacca gcgaattttt gttatactgg
     5041 cgcgcatcaa ccggtcttta tttttttatg caaagtgact attatccaac attttgttcg
     5101 taaggatatg tgcaatgttc ggttttattt cagatttatt aacagcagca gtcagttctt
     5161 tagatgaatt attacaagat actccggcac atcaaatcat attaggcaca gctgctcttt
     5221 attttcttta taatcaatac cataacccat cgatttccag gtggtaccgt agccgcaata
     5281 acgccagtat gaagcagcgt attattgata gtgcttacgc cttggcaaaa aatttacctg
     5341 gtgtgaatca gatcattgag aaagagctca ataaagaact ctcttcaacg agagaaaaat
     5401 tgagaattca gcgttccggt atgactctca gggaggaaat acctgaggaa gggctttctc
     5461 cccaagatat tttgagtgca tttgatgtgg atgtggaaaa atgtcatttt gattttttat
     5521 ccgtaactaa tgacagccct gagagggagt ttcttgttgg aagaggggat ggtaaagatt
     5581 caggtgcatt atacgctatt caccctaagg aactgacaga gctgcttaaa gaagtttatg
     5641 gggcaaccgc tctaactaat cctttgcatg ataaatggcc tcgaattaac gcaatgcaag
     5701 cggaggtgat tcgctggtgt caaaatttgt ttcatggttc aaaagaaggt tatggtttat
     5761 taactcatgg gggtacgacc agcattattg aggcgatggc tgcttatgtc attcgcgcaa
     5821 gggcaaaagg cattgattat cctgaaattg tcgttcctga aacagcacat gctgcattta
     5881 aaaaagcagc cgaattaact ggagcaatat taattacggt tcctgttgat aaaaaaacgg
     5941 gtgcggtgaa tcccaatgtc atgagttcct atattaccag aaacactgct gtaatggttg
     6001 gatctgcccc atcgttcatg aatggaattc atgatcctgt ctctgagtta ggtcaattag
     6061 caaagaaaaa aaatgttcct tttcatgtag atgcctgttt gggtgggttt ttaacggctt
     6121 ttttagacac ttcatccgag cctatggatt ttcgagtgcc tggagtgact tccatttcag
     6181 ccgatttgca taaatatggc tgttgtccaa aaggcacctc tgtttgtctt tttagtgagg
     6241 attcgcctgc tttgtcggtt tatgccgcat taaactggtc aggtggattg tatgctacgc
     6301 caggaattct ggatggttca accagtggag ctcgtgtcgc ggaagtctat gctacattgt
     6361 cttactatgg gaaaaataaa tatcaggaaa tcgccaaaag cattatcaga ttacgcaatg
     6421 ctattcaaaa agaattgacg acattagtgg aagaaggcgc tggattaacc agcgaggata
     6481 tttatgttta tggtaatcct caatggtcca ttctgggttt tagaagtaac acttgcaatg
     6541 cccatttcat tgcagatgaa ttggagaagc gggggtggaa gcttaacttg ctacaaaatc
     6601 cggatgggtt tcatttatgt ttgactcatg tgcacactgt agtaaaaggt ttcgaaacgc
     6661 aatttatcaa tgatttaaga gaggcggtga ttgatgttaa aaattatccc cctggaaaga
     6721 aaccaagtgg taatgtcaaa gtgtatggtg ctgtcggtat gatgccgatt gagcttcaga
     6781 aagaaatttg taaacaatat caaaaagccc gccttgattt tacagcagcc aaccacggga
     6841 gcttggggat atttacacct tcatctacag aagaggacga tggacttaga aacaggaaag
     6901 taggggaaca aaaagtgcaa acctcgcttt gaaccaatag agctcttgca taaccagtct
     6961 ttatcttttt aatgctgcct tgcaaacgtt tctgcggcgc tgatttactg catctaaact
     7021 acgctcgctg agacgtttgc gccttgccgt aaatcaaaat aatcagctta tgcaagaggt
     7081 ttgtttttac tataaaaatt acacgcgttt ctgtacagaa ttttttctgt cagttcctca
     7141 tcaaatagat cccggattaa ttgaatgtca ctatccagaa aagggcgggg agcccgcgtg
     7201 gagggtaaat ctgtaccaaa taacaaagcg tctggattaa tttgggcaat ggtttgcagt
     7261 gcttttttta catcaaaatc gactctccca aaacctgaag cttttatttt gatgccatgc
     7321 tccaccagat ggagtaactc ggaaaaccct gactgtgata atcctaaatg atcaatactg
     7381 acagctggca attccaataa cagattagtc aaatcaccaa gctcctttga gtctacataa
     7441 atctctacat gccaccttac catttcataa atacgatgag caaaatactt taactgatgg
     7501 atattttctg agcctccacg cttgagattg aaacgaaccg ctcgaatgcc ttgttgattt
     7561 aattgaataa tttcttcatc acttacagtg gcaggtaatt gagtaacgcc cacaaagttt
     7621 gggcctaaaa ccgataaagc cgttagcaga taggtttggt caaaggcttg gaatgaacct
     7681 gaaaccacgg ctcctccaca aatattcagg ggctttgccc gttggagata atcttcaaca
     7741 gtaaattcgg gaggaagata ggattgatta gcgactagag gaaaacgata atcaataata
     7801 tgaaaatgag catcaaatag tttcagcatt tcagtcaatt aaatttgcgg ttaaaatcat
     7861 actatcatac aaagatttca ttcgttatta gcgattggtt ttttgcttga tgactataaa
     7921 tgatataagg ccattctcta atttatttgt cagttgacag atagaatact tgttgctatt
     7981 aaattgtaag tggtttttat aatatgggcc gttagctcag ttggtagagc aggagactct
     8041 taatctcagg gtcataggtt cgaatcctat acggcccaat caattattaa tcgcctaatc
     8101 ctcaaaaatt aaaatatgct ttattaattc gtgttattgc taatgagttt tattatagta
     8161 ttgtcattct ttctcatact catatggtct aaacaatgca ccaggagtta ttaaagtatt
     8221 tagaaatgta tcacccgcaa attgcgccat ttatgcatct tcagattaat gaatggggca
     8281 aaaacaggcc actggaaggg gtgaaaatac tgcatcatgt gcctttggtt cccaatacgc
     8341 tgctaaaaat agcagctctt gtcgctgcaa atgctgaggt gaccgtaacc aatccggctt
     8401 ctttttgtca ggcacatcct gatgcgatta cttgtcttca taaatcagga attccttatg
     8461 ttgaaaatct tgatctttta agagatgaaa cgttcgatgt ttatctggat tgtggtgggc
     8521 aactttatca gagtctcgga agacctttga ggggagctat tgagctaacg ggttcagggg
     8581 atcagtatta tcgtagtctc aaaacacttg attttccggt tataagcatt gatcacacaa
     8641 tgacgaaaca attggaaaca gtatttggct gtgctgaaag ttctcaccaa gcgattcaaa
     8701 aactcactgg tgtcgatccc acaaacaaga attggctaat ctttgggttt ggtaaaatag
     8761 ggcgagggct cgcttatttt tgtgtggaac atcaagtacc agttactgta gtggacagtt
     8821 cagtccatca atgtgattta gcttccaatc ttggtataga gtctattaga cctgaggagc
     8881 atgataagct cgaaaaagca gtggctaaag cagatattat tttaactgca acgggcggta
     8941 aaaatatcat gagcaaatac cccagagcct ggtttgatgg caaaatcttg gggaatttag
     9001 ggctgcatga tgagtttggt cccaatttca cctctcagga tgtattatac gataaaaaac
     9061 ctattaattt ttgcctatat gatcctacgc caatgatttt tatagatcct gagttttatt
     9121 tgcataatct gggatgttta ttgcttttac agggaaattt tcctcgggga atgcatgata
     9181 ttcctgaaga tatagattcc tctattatta aagactggtg ccaatatcat aggcgttctg
     9241 aaaaaagtat tcgacgttgg tttctgactt cccaggattt ttctcttaaa acaaattaat
     9301 ataaacagtt cgaataagac ggtttatagc cgctttaatt agtttcctta attgaaaatt
     9361 actttttcag gtcttcttaa tattaataaa atgtggggag ctatctaaaa ctcctgtttt
     9421 ttcaaaatat tccttgtgtt tgattttctt catcaaaaaa cttcacaaag aaggttttgg
     9481 aaagcttggg attggcttga ttgatgcgtg taaattcagc cgtatatcca cattttttgt
     9541 aaaattctgg tgcttgaaag gcgctggtac ataaattgat attattaaac ccttgccctt
     9601 taaaatgatc ttcaagagtt tgcagtagtt ttctgccata tcctttacca cgataagtct
     9661 tatctaccca gatatcatct atgtatattt ctgcaaaggc agtaaaggca ttaataacgc
     9721 cacaaggggt cccttctgca gatagtacta tcgaaaaacg cttgtagttc acatcaatgc
     9781 catgagttcg ttcataggct atcagatctt ctgtcatcct tttttccaga gattcaggga
     9841 tactatccac gaaatctatt gtaataggtt ttgggggaat ggaatgacaa gatttgttct
     9901 gctgttgtgt attaaaactt aaaataatgc gttgggtttg gcgaatatca taattgacag
     9961 tctttatctg ctctttatac tgattaaggg tataggtcat taccgcttga cgccaaaacg
    10021 ataaagccgg tatgttttct ggaataaccg atatttccca ttcacctgga tgagtacgcc
    10081 ataattgatg ggctactgat tgaccaatac ctttcccttg gaatttagcc aggatgaaaa
    10141 attcccccat attccaatca acagcttggg ttccaaattt atttaataag gcaaaaccgg
    10201 ccagttcatt ttcaatttga ataaggaagg cttttcgttc ggggtcagta aaataaattt
    10261 tgaaatcata actttcataa agtccatctg gtggacatgc ccattcttcc gagataaaac
    10321 cgcaataacg tgacatatca taaacataaa atcgtgccat attttgaata actggatagt
    10381 tactaagtgt tgcttcaatt aacttgggct gtaataaatt atcagtgctc atgaatattt
    10441 atactggagt gagtttctta actatacgga taaggtatca aagtgtgctg tggttttata
    10501 gagcgattgt tgttatttgc ataattaatc agcgattttg gtagattgtc tggaataaat
    10561 aaaagatttc catgaatgaa agtggttaat tcttaatatt gatttttata attataggtt
    10621 tctcaatgat atttttagtg agaatgtctc cattgccctt cggcaatacc tctatagatc
    10681 acataagtag agagaattaa aaggatatga tagatatcat tatcatagcg atattctaaa
    10741 ggtatttcat gaccagtgag atagttttca atatgtcttg taactatcca tatcaggttt
    10801 gatataacga ccatgacaaa accaatcctg acttttgctt gcgaataata agccgcaatc
    10861 aaacatacaa ggacgacagg aagggcaatg taggtataat ttccatcgtg ggtcaggaac
    10921 agataagtaa atatgatgga tagaaatact acgataactc ggcacaaatc gggcatttgt
    10981 attgattgga caagcagata attcagcatg attacaagca aactaatcgc aatccaggct
    11041 ggtggtaaat gcaaatgaga agggtagtaa tggacataaa ctcccaatac cttgagaaca
    11101 aggaggttta agaaaaaaaa tacaatcaaa agtgaaaaag tatgatcttt tttaaagtag
    11161 tgaatacagg caccaattaa gtacaataat aggaaaaacg catgaactgc attagagata
    11221 gcatttgttg ttgttgcgtc ccataccatt ctgtatctcc ctttgaaaat acagctatca
    11281 gggtctgata tcctgatgtt tgttaatgta ctaatagtaa ttgtagcttg tagaagattg
    11341 ggatatgtga tatcagtcac ttaaaagcca tactacaagc tatgattagt cattactcca
    11401 attgtttatg ttcgctttag acaaaaaata cgaaggcgat gttcatcggg atcaacacca
    11461 acaaatgtgt acccaaaatc catttgtgtc ggaggttgaa gaatagaaat ttctttatca
    11521 gaccattgct tatatatctc atcgaccatt tcattactac tgacctgaaa ggacagctcc
    11581 ataccccctc ctgtttgatg agctttaggc tcaatctctt cttttgccca taacccaaga
    11641 cgaagccctg ttttcatgac aaacatagcg aatgtcggtg aagattcaat aggttgtgtg
    11701 tctaacagat ttttataaaa ttcttcactt tttgccgggt ttttgacgta aaataaaacc
    11761 aaattagggt cacttaacat gttattttcc ttatttttta ttaatcatcg ccagtataaa
    11821 caaggctcat gacagatttt gtcagtagtg ttttaagtgc ctaagattga ccttcttgat
    11881 aataaaaaac acacgaaaca ggattttggt gttattatga aaacaaaatt tagtctatcc
    11941 tggctcaaaa aaaattgatg agtttatttt atgaacagcc gtattgaaac tttccatcaa
    12001 atggagcggc attttttttc actgattagt gtggagcttt tattattgga aggcttgcaa
    12061 gcttgtaaca gtggaatgcc aattcctgct ctaaatcccg tgttttattc aggtaaatgg
    12121 actctgctta ggccagagat ttatcaatgt aaacgctttt atgagtctca ttcaacccct
    12181 tgggcactgg tggttcctga ctatttatcg gatacagaag cagataaggt gctcgctcaa
    12241 tcaggattct tatctattga tcaaggcgtt gccatgtatt tgccaattgg tgatcttgga
    12301 atttctgagc ttgtatctga cttgttgata aggcgcatgg ataatgagat agatacctgg
    12361 attttaccta ctatccctgc atttgaatca acggaagaga ttgcaaagat ttacagggtg
    12421 agacatcaag aggctattga gaaatccccg gggatttctc atttttccgg gtttattcaa
    12481 gagcaaattg tctgctcatt aactctgact gttatgggga acagtgcgcg aattgatgac
    12541 gtagccactt ttcccaaaca tcagaaaaaa gggtatgcaa cacaattaat tttatctgta
    12601 cttcagactc tcaaagacac caatatttcc tggtgctttt tagaggcatc tgctgatggt
    12661 ttgaatattt ataaaaagat tggttttcgt gaactgttta aaaatcttta ttatgaagag
    12721 caatgccaat atgagcaaaa ctgagagact ctttcatctg ttgcaaattt tgcggcgtta
    12781 ccgttatcct cttagcggac aatttcttgc caatgagttg gatgtaagta tccgtaccat
    12841 ctatcgggac attgcggttt tacaggctca aggcgctgat atagagggtg aaccaggatt
    12901 gggttatgtg ttaaagccag gttttatgtt accgcctttg atgtttaatg aagaggaaat
    12961 tgaggctctt gttttaggct ccagatgggt ggctaaaaaa acggatactc acttgcaaca
    13021 ggctgctcat aatgctttgg ctaaaatcgc ttctgtactt cctgccgatt tgcgacagca
    13081 attggagcta tccagtttat taatagggcc tggtgaagtg atttcaggag aagatgaatc
    13141 cctggcttta gtcaggaagg caatacgaaa agagctgaaa ttgtttattc aataccatga
    13201 cttaaaagga aatgtttcaa cacgtacgat ttggccaatt ggacttgggt attttgatca
    13261 agtaagagtt ttgattgcct ggtgtgaatt aaaaaaggat tttcgtcatt ttagagccga
    13321 tagaattgta gatgttgaaa acttgggggt ctcttatgca aagcgacgct cggtgttgct
    13381 tcatgattgg cgaaagcaaa ataatatccc aggttgatga caacacgatg ttattggcgc
    13441 gttaacttaa acaggtaaaa tttaatgccg ggaatcaagc tttactttct tcccggatta
    13501 agattgcttc ttatgcgaaa gacgtgctga actccctctt attcctgagt gagagaacct
    13561 cgtatttcaa acggttttac atcaaatttt gaatggcaat ggctgcgaca ccaacagcaa
    13621 ttgctcctaa tgtttctcga tttggcaagt gctctttaaa gaaaccataa taatgattta
    13681 aatttgaaga gaaatcgctg ccattggttt catgtttgat ttctgatagc acttttgtaa
    13741 cggaaggctc tttttgttca acagcagtgt ccttttgtag tttttcttgc agtttttgtt
    13801 ctaacctttg ttcaagttta tgttctaact gtttcaattg atattcgttg atttgctttt
    13861 gattcttatc gcattcaatg tgagtttctt ccagttgttt ttttaattca gctcgatagg
    13921 catccgtggt gtcgaagggg tgaaattctt gcttcagctc atgttcagca ttaatgactt
    13981 gctcatagct ggtttttaaa tttttgataa atgaatcatt agaaaaccag gacatgatcc
    14041 agtcgaaaaa ttttgtaaaa aaccatttgt tattccattc atcgtttaat ttatggtggt
    14101 tttgcgaaag gagatgatag tctgccagtt tctttgcaag agcatctatt ttgacaatca
    14161 attgatttct tttatttctt gaatcttcaa gggatttttc aagcttggct agttcatcgg
    14221 acaggttgga ttcttttgat tcattaagtt gttttagccc gattggttca ctttttgcag
    14281 ttttatcggc atgaaaattc ttgaacaatc ttgttgtata ctcttttgct gactcgtcat
    14341 ttttctttat gaattcagat tcattaaagg caatattttg actttgacaa tatcgtctaa
    14401 tttctcccaa catcgcttcg gcttgagcac gggtcgaaat tttcttttca ttaacaccat
    14461 ggattacctt tgatctaaac caattgatag aaccttggta ttcgacattt tcatagttct
    14521 taaggtgctg ataataaacg attgcaaaaa tcagagtctc agcattagtt attttgggta
    14581 aacaaaattc atattttttt tcaggggtaa caaatgggac tcgatatccg aacaatttgg
    14641 ctttttcgtg caatcgatct ccaacctgct ggatttgagt aatcccgagt tgttctattg
    14701 tcaagccaat ctctatcata aggtccgcga cggctatttg attggagaat tctttgttgc
    14761 ttacagatga acgaagttga tcaatgcaat ctttaaattg ttgccaataa gttgttattg
    14821 aaatcatatc aatcattatt taagaaaaaa gaatcataaa tttacaccgg tcttctaaaa
    14881 aaagctatta tttagtcgtg attatcaatc agaaagtttt taaaaagaga gaaagtaggg
    14941 gagacagttg atatagggga aaagagctat ttaaaattgt ttgccaaaag taataccaaa
    15001 gatcagctct ttttcaccac cttcaataaa atcaaggtct atattcggag tgatttctat
    15061 atccggaggg aaatgtatga tatatccaat ccccaatcga ccaaccatgc ggttgggttc
    15121 atctttttca tatctaattc cagggcctat gccaaaagtc aggttcctga atacgtttaa
    15181 tgtaccaagg cctatcaatt ctacttgatt attatgttcc acattgacgg gtgtatgttc
    15241 aataaccgca ctaaacccaa aaggcatgga aaggactcgg tggtactcaa atccataggt
    15301 aaacgcttca tcattttgag catgatttcc acctacaaaa acaccaagga gatttttgtt
    15361 tttttccctc acgtgcggtt tgtttgagaa atcaggagcc gaatttaccg ggtgtattga
    15421 acaaaaaagg catgcaatcc ccagaatgat agttattatt ttcttaccat ttattaccga
    15481 catgagttct caatacaggg ttatctattt ctttattaaa tcgcaaaatg gctaatattt
    15541 aaagaacatt tgaatcgata tgttatataa aaatctttat taacaataag atgttgatga
    15601 tgtggctggc aaaaaatttt ttaagacatt gccattaatt tttctacctt aattgttgat
    15661 tattaagtaa actgatgatt tt
//
LOCUS       DATNSN010000074        15521 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.074, whole genome shotgun sequence.
ACCESSION   DATNSN010000074 DATNSN010000000
VERSION     DATNSN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.074"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(22..1689)
                     /gene="recN"
                     /locus_tag="VAH76_001816"
     CDS             complement(22..1689)
                     /gene="recN"
                     /locus_tag="VAH76_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO1386430.1"
                     /translation="MLTTLRIEHFAIVKQLELDFTQGMTTFTGETGAGKSIMIDALLL
                     ALGEKADTSVVRPGEEKCDITAGFCFDESSELAQWLAEHDISCHDGEIYLRRIIYAEG
                     RTKSYINGQPFPLQKIKELSEKLVHIHGQHQHQTLMQHATHRQQLDRYANNHLLLNEV
                     TTLYKQYQQIVQEINALQSQEQQADRIKLLQFQIDELQTLGIREGEVEALHQEHQILH
                     HAREYLQLSQQINELLNSDDQPNICSGLNQIFQLIQQLPQEQAQIKNTAELINSALIQ
                     CEEAITEMQQFADKIQLDPERLQEVEARISSIHQLARKYHLDSKSLQEHEKTLQNELE
                     SLQNVESKLAHLHLQLAQINKAYESAALKLRESRKIHAKKLAQEITASIQQLGMPKGW
                     IEIEITPLEKMQAHGLDKVEYKVCTNPGMEPDSLNKIASGGELSRISLAIQMITAQKG
                     STPTLLFDEVDVGIGGSTAALVGKMLRKLGERLQVFCVTHQPQVAASAHHHFLVTKHS
                     NNNQTFTHISLLKASDKIAEIARMMGGLTITEQTLSHAKELISLSNE"
     gene            complement(1693..2580)
                     /locus_tag="VAH76_001817"
     CDS             complement(1693..2580)
                     /locus_tag="VAH76_001817"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEO1386431.1"
                     /translation="MKQKFKRAILYARQHRANQEVNESLHRLVDFLSTQDIEIFQDSD
                     TAASFELKAPVLPREKMGEKHDLIIVVGGDGSLLSASRMAIKVNTPVIGINRGRLGFL
                     TDILPQDIESHLGPVLNGQYNEEERFLLHTKIYDKENSYFEGDALNDVVLGRGSETHL
                     IEFDVYINQQLVSHYRSDGMILSTPTGSTAYALSAGGPIMHPQLNAIVLVPMFSHSLS
                     SRPLVIDGEAEIELYISKSNETDLRISCDGHESRVVKPGQKVAVKKNGNRLRLLHPLD
                     YHYYDTLRSKLGWESKHQG"
     gene            complement(2587..4413)
                     /gene="typA"
                     /locus_tag="VAH76_001818"
     CDS             complement(2587..4413)
                     /gene="typA"
                     /locus_tag="VAH76_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO1386432.1"
                     /translation="MIEKIRNIAIIAHVDHGKTTLVDKLLQQTGTLNERAPKVERVMD
                     SNALEKERGITILAKNTCVYWKGYQINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFARGLNPIVVINKIDRPGARPDWVMDQVFDLFDNLGANDTQLDF
                     PVVYTSALNGYAKLNLEDNATDMSALLQTIVDKVTPPCVDANGPFQMQISSLDYSSYV
                     GTIGIGRITRGQIKAKSPVKIIDKDGNMRSGRLLQLLGFKGLERVEVEEASAGDIIAI
                     TGIESLNISDTICDPNLVEALPALLVDEPTISMTFQVNDSPLAGQEGKYVTSRKIRER
                     LNTELLHNVALRVEDCDDPDKFRVSGRGELHLSILIETMRREGYELAISKPEVIIREV
                     DGEQQEPYEHLTVDVEENHQGTIMEKLGERRGEMQNMVPDGKGRVRLDYIIPTRGLIG
                     FHNEFLSCTSGTGLMYHVFDHYGPLIKGRIGKRLNGVMIANCPGTARAFALFNLQERG
                     RLFLEPQSVCYEGMIVGIHARDNDLVVNVTKEKQLTNIRASGSDENIILTPPVKLSLE
                     QALEFIDDDELVEVTPLSIRLRKKFLKEHDRKKASRTATSEE"
     gene            complement(4525..5337)
                     /locus_tag="VAH76_001819"
     CDS             complement(4525..5337)
                     /locus_tag="VAH76_001819"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEO1386433.1"
                     /translation="MDYYFTKGKPQLVNMKTPIYLVTQFQQLMDLMQNQYEVSCLELM
                     QRSGKAACDFLVYRWPKVKKISIFCGRGDNGGQGYVLAQQAKKMGMVPTVWQVGHQMS
                     MSKPPQMHKEVWYEMNSCHQQGIVLHTYSPDIDLGDPELIVDALFGVGLYGHVRPEIA
                     LLLQRLQQFTVPILAIEVPTGINASTGEIAGNALAATATITFLCMKLGLLINDGKIYS
                     GEIAFDDLLAPEAIYQQVKGIEESSLLDSSTIFSKKIWYRNKTQKGWQLSIN"
     gene            complement(5445..6455)
                     /locus_tag="VAH76_001820"
     CDS             complement(5445..6455)
                     /locus_tag="VAH76_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO1386434.1"
                     /translation="MKYRKLGKVGPDVSALGFGCMGLSEFYGKPARLDDAVKIIRNAH
                     DQYQVNFFDTADIYGKGKNEELVGKAIKPFRNQIILATKCGVVRTGSNDEMSVNNSSA
                     YIRTACEKSLKRLNTDHIDLYYLHRYDHQTPIEEVMGIMHDLIDEGKIGYIGLSETDA
                     DTIRAAYTVVKDKLVAVQTEYSLRVRAPATAVLGTCRELGICFVAYCPIARGFLSGKI
                     KKPNLLGTNGFDFRTNVPQFQPENFAQNLGLVNELEAIGEKTGYTPAQLSLAWLLAQG
                     EDIIPIPGTSNPQHLAENMHSIDIFLTPEQMKDLENAYKNNPVAGKRLSKELMTAFHL
                     KE"
     gene            6633..7592
                     /locus_tag="VAH76_001821"
     CDS             6633..7592
                     /locus_tag="VAH76_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO1386435.1"
                     /translation="MSFKGFKVVMLILLSTQSYASKLASSSVCDSTIENPCIVQDSKT
                     QFSPVIRYREVASIADVYGGNITGINKFHLSGSEQPSEKGWEAIAESISRKMGAEAKK
                     VIVLDLRQESHGYLNGRAITLVSVYNWINLGKSNSQSTLDQENWLTGLRSRKIVNGVL
                     TVPQYVAKQYSQGKSMVVSTIKNEEYYVYKKGFDYYRIFISDHRAPLDSEVDALVALI
                     KNNPEDTWYHVHCRGGKGRTTTVFAMFDMLKNADKVSFEEIIARQASIPPFYNLMVTN
                     REIPELTPYYEQRLQFLIHFYEFARQSLMGYSGTWSEWKKLNI"
     gene            7860..8357
                     /locus_tag="VAH76_001822"
     CDS             7860..8357
                     /locus_tag="VAH76_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386436.1"
                     /translation="MKQLILHPTDISQWHALVNEAQAATRLILNENTESYLVFLLMRF
                     SQGTKLIESVVALDFLESMRKPRQLQVELLRDVGDKSLLFCGLFPGIAKKRHVSLSYF
                     SDMGQAAYLTVGELEENQTANLYLQLSEQFITLQQVLQAMRGEYIQFSQSSCGVLADP
                     DLTMQ"
     gene            complement(8371..9216)
                     /gene="hslO"
                     /locus_tag="VAH76_001823"
     CDS             complement(8371..9216)
                     /gene="hslO"
                     /locus_tag="VAH76_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO1386437.1"
                     /translation="MKEADTLQRFIFEHANIRGEIVHIEKTFQNIMNQRDYPPMVKNL
                     LGEALVSCLLLASSIKFEGNLSLQFQGDERLSLLLVQCDHNLNIRGFAKCAEGLEIAD
                     YATAFLQGRMVITISQDNQTQAYQSLIPIQSTSMSENLMTYFAQSEQIATRVWLAVNE
                     DMAAGMLLQLMPGQDTIQREQFWEYAVQLGQTVTENELLTIDNQTLLYRLYHETELRI
                     FESRSTRFQCRCNQEKMKQVITVLGEEEATDLIKEKGKIEITCDFCNQKYTFDSIDVT
                     LLFRK"
     gene            9424..9573
                     /locus_tag="VAH76_001824"
     CDS             9424..9573
                     /locus_tag="VAH76_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386438.1"
                     /translation="MKNNSLLLPLFKTIDKLLFGIEEIKEIEKTPEYILCDNEDEFDV
                     ERSIN"
     gene            9632..11629
                     /locus_tag="VAH76_001825"
     CDS             9632..11629
                     /locus_tag="VAH76_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEO1386439.1"
                     /translation="MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGY
                     NANSICAVTFTNKAANEMRARVSAFLPAVSRRGLKVATFHTLGLSIIKRDVSRCDLKP
                     GFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
                     CDEALTIYPRYQEALKAYNAVDFDDLIRLPVDLLNDHADVLEYWQNKIRHLLVDEYQD
                     SNTSQYLLVKKLAGVRAHFTVVGDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQN
                     YRSTSRILHAANHLIAHNQHLFEKKLWSDLGHGELLRVVSCKDEQDEAEHVIADLISH
                     KLRNRTNYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKL
                     LCNEADDAAFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLALSEFVADKQR
                     MIFHDFKLWMEDIKKRISSDSVLEHLRQMVEDIGYEAYIYEQCDSPAKAQKKMDNVWE
                     LLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGLEFPFVY
                     LVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPS
                     RFLDELPQESLEWFGKSGERCEEQSKKLAKSHLEGLKNLLS"
     gene            complement(11713..13695)
                     /gene="iroT"
                     /locus_tag="VAH76_001826"
                     /gene_synonym="mavN"
     CDS             complement(11713..13695)
                     /gene="iroT"
                     /locus_tag="VAH76_001826"
                     /gene_synonym="mavN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector ferrous iron transporter
                     IroT/MavN"
                     /protein_id="HEO1386440.1"
                     /translation="MNIKNIPIKPQKIPYYLFLLLLTVGASLILGFLSFGGMYALWPV
                     LPLAFAGFALSVAYEGEIYLQNIKGALNKLFKFNYLKNHFAKEYLLNHFPNTNEENCP
                     QFFKDYKRQLKLLAEFGHKELNQDSKKRKRQIEKTLSDMEKWFALQLFSDNNNPSPNT
                     SKYARELLDWLALNDQDEWIKRWEKRHFQFHIVKGFSVVAGLFMGLGSTYLIVEAFTI
                     IPFFAAIPFTFWPIIILPMAIVAGAAYGMLTYNAVTDMINNNTVVKWYNKIRDDLSQG
                     LTVRNVFIASTAVFLVGLAVALTICTAGTWWTIATNARPLFDWMKKMPSFIMGIINPI
                     ITGASAIVFNIQNTAESLDMVDEATRSNKNVFQKIYEAISNGYQHLRETENWLQIVNP
                     FRILLKLTITPLRILLFLGHLISVAVTSDRMPGVPQILSALVAIISEGFEDAHYFIGH
                     THEDEQDEELGHQDHHQFEKLLKERLAPDSDQDHNMDIPTWILKTIASPIYGLAALWD
                     CSASKLNPPQENHSKNLPSGKKPYVLSLGEAWNKQWGVAKEFNVELSTHAKRPSQEWQ
                     VEHAVAQIDKFQRKHLKDIVIGRELADKKVIALNHLKEKIRHPEKGETLSDTLEQAKK
                     QPDYNQHRLFNQKGEKTRTQVFIEELPERINLSSGK"
     gene            13877..15082
                     /locus_tag="VAH76_001827"
     CDS             13877..15082
                     /locus_tag="VAH76_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO1386441.1"
                     /translation="MRFNQWMTCITSGLILASNSSFATTSPVHEQLQVPQCLAAKITV
                     PHKILAENKEFKIIDVHSSDVETLTILADKVSCGHFVNVSHKLTGTLAANQQQSAQKL
                     LQKKLVKPLGVSKLHKDVYEIKHEEEVNAALKEIVSDNIWQTLTHMTSYYNRSATKDT
                     GVETANWLKLKFEQMAVEYGRTDTSTFFVKTGWYKQPSLVTVIGKDIKAPAIVIGAHM
                     DTLDGRMPGAGDDGSGSSSIMEAARVILSSKTTFKRPIYFIWYAAEERGLVGSQHVVQ
                     HFQEQSIPVKAVVQFDMTGYRNDANDPTMWVFTDYTDRDLSNYLAKLIDHYIHVPVDY
                     SRCGYGCSDHASWNEEDIPAAFPCETSFADHNPYIHTSSDKMDLLNLEHMTNFSKLAV
                     AFAIELASE"
     gene            15278..>15521
                     /locus_tag="VAH76_001828"
     CDS             15278..>15521
                     /locus_tag="VAH76_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386442.1"
                     /translation="MSKIIDSLKNSDVPHLYLLNIGLTREEYYDTSKMSRDEKRQLVN
                     NIIMKASHEEILKIINDLMALELSIESNDPIRTGNRL"
BASE COUNT         4508 a         2912 c         3142 g         4959 t
ORIGIN      
        1 acctgaaaac gatcgatgtg ttcactcatt actcagcgat atcagctctt tcgcatgaga
       61 cagtgtttgt tctgttatag tgagcccacc catcatgcga gcgatctcgg caattttatc
      121 ggatgcttta agtaatgaaa tatgagtaaa agtttgattg ttgttactat gtttagtgac
      181 taaaaagtga tggtgtgccg aggcagcaac ttgtggctgg tgggtaacac aaaaaacctg
      241 caatctttct ccaagttttc tcagcatttt tccaactagt gcagcagttg aaccgccaat
      301 acccacatcg acctcatcaa ataataaggt aggagttgaa cctttttgtg ctgtaatcat
      361 ctgtattgca aggctgattc gggataattc tccgcctgag gcaattttgt tcaatgaatc
      421 aggctccata ccaggattag tacatacctt gtattccact ttatccaatc catgagcttg
      481 cattttttct aaaggagtga tttcaatttc aatccatcct tttggcattc ctaactgctg
      541 aatgctggcg gttatttctt gagccagttt ttttgcatga attttgcgtg attctcttaa
      601 ttttaatgcg gcgctttcat atgccttatt gatttgagcc aattgcaaat gaagatgtgc
      661 caatttgctt tctacatttt gcaaactttc caattcattt tgtaatgttt tttcatgctc
      721 ctgaagcgat ttgctatcaa gatggtactt ccgggctagt tgatgaatag aactgattcg
      781 ggcttccact tcttgcaatc tttccggatc cagctgaatt ttatctgcga attgctgcat
      841 ttcagtgatt gcttcttcac attgaatcaa ggctgaatta attaactcag ctgtgttttt
      901 tatttgcgct tgttcctgag ggagttgctg gatgagttga aaaatctggt ttaaaccaga
      961 gcaaatattc ggttgatcat cactattcaa tagctcattg atctgttgac ttaactgcag
     1021 atactctctg gcatgatgca aaatttgatg ctcttgatgt aaggcttcga cttcaccttc
     1081 gcgaataccc agtgtttgta attcatcaat ttgaaattgc aataatttaa tgcggtctgc
     1141 ttgttgctcc tgactttgta atgcatttat ttcttgtact atttgttgat attgtttata
     1201 gagtgtagtg acttcattta acaagaggtg gttattggca tagcgatcaa gttgttgcct
     1261 atgtgtcgca tgttgcatga gtgtttgatg ctggtgttgc ccatggatgt gtaccagttt
     1321 ttcacttaac tcttttattt tttgtaaagg gaaaggttgg ccattaatat aagatttggt
     1381 gcggccttct gcgtaaataa tgcgccttag atagatttca ccatcatgac aagaaatatc
     1441 gtgttcagct aaccattgag cgagttctga tgattcatcg aaacaaaatc cggctgtgat
     1501 atcacatttt tcttcgccgg ggcggactac ggaggtatca gctttttcac ccaatgctaa
     1561 cagcaaggca tcaatcatga tggatttgcc agcgcctgtt tcgcctgtaa aggtggtcat
     1621 gccttgtgta aaatcgagtt ccagttgttt aacaatagca aaatgttcaa tgcgcaatgt
     1681 cgtgagcatg ttttatccct gatgctttga ttcccaacca agtttgcttc gtaatgtatc
     1741 ataataatga taatcaagag ggtgtaacaa acgcaatcga ttaccatttt ttttgactgc
     1801 tactttctgt cccggtttaa cgacacggga ttcgtgacca tcacaactga tacgcaaatc
     1861 ggtttcattt gatttgctga tatacaattc aatttctgct tccccatcaa taacgagtgg
     1921 cctggaactt aggctgtgtg aaaacatggg taccaggaca atggcattga gttgaggatg
     1981 cattatcggt cctcctgcag acaaggcata ggctgttgag ccagttggag tggataaaat
     2041 cattccatct gaacgataat ggctaaccag ctgctgattg atgtaaacat caaattctat
     2101 caaatgcgtt tcacttcctc tgcctaatac gacatcattg agtgcatcgc cttcaaaata
     2161 actgttttcc ttatcatata ttttagtatg caataagaag cgttcttctt cattatattg
     2221 tccattaagc acagggccta aatgggactc aatgtcttgg ggtaaaatat ccgttaaaaa
     2281 acctagcctt cccctgttaa tcccgatgac cggtgtatta acttttatag ccatacgtga
     2341 ggcagaaagt aaactgccat caccacccac gacaataatc aagtcgtgtt tttcccccat
     2401 cttttctcta ggtaaaacag gggcttttaa ttcaaaactg gccgcagtat ctgagtcctg
     2461 aaagatttct atgtcttgcg tgcttaaaaa atcaaccagt cgatgcaagc tttcattcac
     2521 ttcctgattc gctctgtgtt gacgggcata caagatggct cgtttaaatt tttgtttcat
     2581 aatgtgttat tcttcacttg tggcggtacg agaggctttt tttctatcgt gttccttgag
     2641 aaatttttta cgtaaacgaa tagataacgg ggtaacctca actaactcat catcatcgat
     2701 aaattccagc gcttgctcaa gagataattt gactggagga gtcaggatga tattttcatc
     2761 cgaacctgat gccctgatgt ttgttaattg cttttctttg gtgacattaa caaccaggtc
     2821 gttatctctg gcatggatac ctacaatcat tccttcatag cagactgatt gaggctctaa
     2881 aaataatctt ccacgttctt gtaaattgaa caaggcaaaa gcccgggcag taccaggaca
     2941 attagctatc atgacgccat tgagacgctt tcctatgcgt cctttaatca acgggccgta
     3001 gtgatcaaag acatgataca ttaatccggt acctgaagtg caggataaaa attcattgtg
     3061 aaagccgatt aatccgcgag tcgggatgat gtaatccaaa cgtactctgc ctttgccatc
     3121 aggaaccata ttttgcattt cgcctcggcg ttcccccaat ttttccataa tggtaccttg
     3181 atggttttcc tcaacatcaa ctgtcaagtg ttcataaggc tcttgttgtt caccatctac
     3241 ctcgcgaata atcacttccg gtttgcttat tgccaattca tagccttcac ggcgcatcgt
     3301 ttcgataaga atcgacaagt gtaattcccc tcgaccagaa actctgaatt tatcagggtc
     3361 atcgcagtcc tctacgcgaa gcgccacatt atgcagtaat tctgtattta agcgttcacg
     3421 aatttttctt gatgtgacgt atttaccttc ttggccggcc agcggcgaat cattaacctg
     3481 aaaagtcatg ctgattgtag gttcatctac caaaagtgct ggcaatgcct ccaccagatt
     3541 gggatcgcaa attgtgtcgg atatattcag gctctcaatt ccggttatcg cgataatatc
     3601 accggcactg gcttcctcga cttcaacgcg ttccagccct ttgaatccca gtaactggag
     3661 tagtcgtccg ctgcgcatgt taccgtcttt atcaataatt tttacaggag atttcgcctt
     3721 aatttgccca cgtgtaatcc ggccaatacc tatggtgcca acataagaag aataatcgag
     3781 agagctgatt tgcatttgaa acggaccatt ggcatcgaca caaggtggtg tgactttatc
     3841 tacgattgtt tgcagtaggg cagacatatc tgttgcattg tcttccaaat taagtttggc
     3901 atagccattt aaagcagagg tataaactac aggaaagtct agttgggtgt cattggcgcc
     3961 taagttatca aacaagtcaa acacttgatc catgacccaa tcaggccttg ctcctggacg
     4021 gtcaatttta ttgatcacga ctatagggtt caagccacgg gcaaatgctt tctgggtgac
     4081 gaagcgggtt tgtggcatgg ggccgtcaac tgcatcgacc agcaacaaaa cgctgtcaac
     4141 catagaaaga atacgttcca cttcgccgcc aaaatccgca tgccctgggg tatctacaat
     4201 attaatttga taacctttcc aataaacgca agtgtttttg gctaagatag taattccacg
     4261 ctctttttca agtgcattgg agtccatcac acgttcaact tttggagctc tctcattgag
     4321 cgttccagtt tgttgcaaga gcttgtcaac aagagtagtt ttgccatggt caacgtgggc
     4381 aataatggca atattgcgaa ttttttcaat cataaaaaac ctttagttgg ggaatagcga
     4441 gatttaataa tttaaggtaa taactgacaa ttgttcatag atttaacatt atacactaat
     4501 cgctcattaa agaatatcta ctctttaatt gatgctcaac tgccagcctt tttgagtttt
     4561 attgcgatac caaatttttt tggagaagat agtagaacta tcaagcaagc ttgattcttc
     4621 tatgcctttg acttgttgat aaattgcttc aggtgccagt aaatcatcga atgctatttc
     4681 tccactataa atttttccat catttattaa taagcctaat ttcatacaca agaaggtaat
     4741 ggtcgcagtt gctgccaagg cattacctgc tatttcacct gtgctggcat tgatgcctgt
     4801 aggcacttcg atagccagga taggtacagt gaactgttgg aggcgttgaa gaagcaaagc
     4861 tatttccggg cgaacatgac catacaaccc tacgccaaaa agggcatcga caatcaattc
     4921 aggatcgcct aaatcaatgt caggcgaata agtatgcaga acaatacctt gctgatggca
     4981 tgaattcatt tcgtaccata cttctttatg catttgaggc ggttttgaca tactcatctg
     5041 gtgtcctact tgccaaactg ttgggaccat tcccattttt tttgcctgct gagctaaaac
     5101 atagccttgt cctccgttat caccacgacc acaaaaaata ctgatttttt taactttcgg
     5161 ccagcgatac actaaaaagt cgcaggctgc tttgcccgaa cgctgcataa gttcgagaca
     5221 agaaacttca tattggtttt gcattaagtc catcagttgt tgaaattgtg taacaagata
     5281 aataggcgtt ttcatgttga ccagctgtgg ttttccctta gtaaaataat agtccatata
     5341 aaaaaatctg ttctaacttt tgtaaacatg gtttttagag tgttggagag tttcctgatt
     5401 ggtggttaag aaagcgtcat tctcctatgc tattcaaatg cagcttattc ttttaaatga
     5461 aaggctgtca tcagttcctt ggataatcgt tttccggcaa caggattatt tttgtaagca
     5521 ttttccaaat ctttcatttg ttcaggagtt agaaaaatat caattgagtg catattttct
     5581 gctaagtgtt gtggattcga ggttccggga atggggataa tgtcttctcc ttgagccaat
     5641 agccaagcta atgataattg tgccggggtg taacctgttt tttctcctat ggcctcaagc
     5701 tcattgacta agccgagatt ttgagcaaag ttttcaggtt ggaattgcgg aacattagtt
     5761 ctgaaatcaa agccatttgt ccctaaaaga ttgggcttct taatttttcc actgaggaaa
     5821 ccacgtgcta tggggcagta ggcaacaaaa caaataccca gttcacggca ggtgcccaaa
     5881 accgctgtcg ccggagctcg aactcgaagt gaatattcag tttgaacagc aaccaatttg
     5941 tccttgacta ctgtataagc tgcccgaatg gtatcagcat cagtttcaga taatccaata
     6001 taaccaattt tgccttcatc aataaggtca tgcattatcc ccatcacttc ttcaattggt
     6061 gtttgatggt cataacgatg caaatagtag agatcaatgt ggtcagtatt taagcgtttt
     6121 aggcttttct cacaggctgt tctgatataa gcagatgaat tattgacact catttcatca
     6181 ttagagcctg tccttactac tccgcatttc gttgcaagta taatctggtt acgaaagggt
     6241 tttatcgctt taccaactaa ttcttcattt ttaccttttc cataaatatc agctgtatca
     6301 aagaaattaa cttggtattg gtcatgcgca ttacgaataa ttttgactgc atcatcaagg
     6361 cgagcgggtt tgccataaaa ctctgataat cccatgcacc cgaagcccaa tgctgagaca
     6421 tcaggcccca cctttcccaa tttgcgatat ttcatggttt atcctcaact gacatggttt
     6481 tatttggaca gttgaataca gtatagaatt atttggcagt aacgtattaa ggattgtgat
     6541 tttatttctg aaatggtaac gcgtcttgaa taataaaaat cttttacata gaatgctgcg
     6601 gttcatcttt agggatttaa aaaggattta ttatgagctt taaaggattt aaagtggtaa
     6661 tgctgatatt attatcaact caaagttatg cctctaaatt ggcatcctcc agtgtttgtg
     6721 attcaactat tgaaaatcca tgtattgtcc aggacagtaa aacgcaattt tcacctgtca
     6781 ttagatatag agaggttgca tcaattgcgg atgtttacgg tggcaatatc acggggatta
     6841 ataaatttca tttgtcagga agcgagcaac caagtgaaaa gggttgggag gctattgccg
     6901 agtctatttc cagaaaaatg ggggctgaag caaaaaaagt catagtgctc gatcttcgcc
     6961 aggagagtca tggttatcta aatggcagag caattacttt ggttagcgtc tataactgga
     7021 taaatttggg caaatcaaat agtcagagta ctttggacca ggaaaactgg ttaaccggtt
     7081 taagatccag aaaaattgtt aacggtgttt taactgtgcc ccaatatgta gccaaacaat
     7141 attctcaagg caaatcgatg gtagtgagca ctataaaaaa tgaagaatat tatgtatata
     7201 aaaaaggatt tgattattat cgcatattca tatccgatca ccgggctcct ttagattctg
     7261 aagtggatgc gcttgtcgca ctgataaaaa ataatccgga agacacatgg tatcatgtcc
     7321 actgccgagg cgggaaaggc cgcacgacga ccgtttttgc catgtttgat atgttaaaga
     7381 atgcagacaa ggttagcttt gaagaaatca tcgcacgcca agcctccatt ccgccttttt
     7441 ataatttgat ggtgacgaat cgtgaaatcc ctgagttaac tccttattat gagcaaagac
     7501 tgcaattttt gattcatttt tatgaatttg ctcgccaatc cctcatggga tattcaggta
     7561 cctggtcgga atggaaaaag ctcaatatct agcatctagt gccatttgca agaagatcat
     7621 attaaaaagc cttcatttga aatgaagcga atttcatgaa ggcttggcac tcaatcgttt
     7681 ttggcaaata ccatatcaat cttaaattaa gtgttggttt tctcatgatt tatctgcccc
     7741 aagataagaa aaccttgatt tcatcataaa aatgcattac gaattattat attagtcata
     7801 gaaaacaatt ggtatttgtt tgctagacta atagtaagag gtaaatatgg ggggtattta
     7861 tgaaacaact aatattacat cccactgata ttagccaatg gcatgcgctg gttaatgaag
     7921 cgcaagcagc taccagactg atattgaatg aaaacacgga aagttatttg gtttttttgc
     7981 tgatgcgctt ttctcaaggc acaaagctta tcgaatcggt tgttgcactt gattttcttg
     8041 aatccatgcg caaacctcgc cagctacaag tcgaattatt aagggatgtg ggggacaaaa
     8101 gcttattgtt ttgcggctta tttccaggta tcgctaaaaa acgccatgtg agtttgagtt
     8161 atttttctga catggggcaa gcggcttatt tgactgtcgg agagcttgag gagaatcaga
     8221 cagccaattt gtatttgcaa ttaagcgagc aatttattac tttgcagcaa gtactgcaag
     8281 ccatgcgagg agagtatatc cagtttagtc agtcaagttg tggcgttctg gcggatcctg
     8341 atttgactat gcagtaggca gaggctcatt ttatttgcga aatagcagag tcacgtcgat
     8401 tgaatcaaaa gtgtattttt gattgcaaaa atcgcaagtg atttctattt ttcctttttc
     8461 tttgattaaa tctgttgctt cttcttctcc cagtacagta atgacctgtt tcattttttc
     8521 ctgattacaa cggcattgga aacgggtaga tcggctttcg aatattctta attccgtttc
     8581 atgataaaga cgatagagta gtgtttgatt gtctatggtc agcaattcat tttcagttac
     8641 tgtttgtccc agctgaacgg catactccca aaattgttct ctttgaattg tatcttgacc
     8701 tggcataagt tgtaatagca tacctgcagc catatcttca ttgacagcta accacacgcg
     8761 agtagcaatt tgttctgatt gggcaaaata agtcatcaga ttttcgctca tggacgtaga
     8821 ttgaataggt atcaggcttt gataggcttg agtttggttg tcttgactga tcgtaatgac
     8881 cattcggcct tgaagaaaag cagtcgcgta atcagcaatt tcaagaccct ctgcacattt
     8941 tgcaaagccc cttatattta aattatggtc gcattgaacg agtaacaggg ataatctttc
     9001 gtcaccttga aattgcagac ttaaatttcc ttcaaattta atgctgcttg ccaggagtaa
     9061 acatgaaact aacgcttcac ctaaaagatt ttttaccatg ggtgggtaat cacgttggtt
     9121 catgatgttt tgaaatgttt tttcaatatg aacgatttcg ccacggatat tcgcatgctc
     9181 aaaaataaaa cgctgtaagg tatctgcttc tttcatttca aactcataac acaaataggt
     9241 ctattctaac ataaaatgtt tatctcaggc cttttctgag ttcagttatt ttgaacaaaa
     9301 tcttgcaaga ggaatagtag tttccataac cagcgtatct taattatttt ttaattttcc
     9361 tggagaaatt tatttcatgg actattctta aaaaacccct ttttaaaatt gtaaggagtt
     9421 attatgaaaa ataacagttt acttcttccc ctatttaaaa caatagacaa attactgttt
     9481 ggtatagaag aaattaaaga aatagaaaaa acaccagaat atattctctg tgacaacgaa
     9541 gatgagtttg atgtagaacg ctctatcaat taattcttgc gtgaaaaaga aagcttgggt
     9601 gataatgcct tttttttgag cggaaataag tatgttgaat agccaacaaa tggctgcagt
     9661 aaaatatatt gatgggcctt tgttggtgct tgccggggct gggagtggta aaaccagagt
     9721 tattacacaa aaaattggtt atctgattaa ttcatgtggt tataatgcca actccatctg
     9781 cgccgttact ttcacgaata aagcggcgaa tgaaatgcgc gcaagggttt cggctttttt
     9841 gccagccgta agcaggcggg gattaaaggt tgcgacattt catacattag gcctaagcat
     9901 catcaagcgt gatgtttcta ggtgcgattt gaaaccggga ttttccattt ttgatagtga
     9961 agattgtttg caaattttgc ggggtttttt acctgttaat aaggcaaccg atagagattt
    10021 tatcctcaaa gttcaacaac aaatttctct ttggaaaaat aatttattga atcccgaatt
    10081 aatactccaa aaccctccgg aaacactttt atgtgacgag gcgttaacaa tttatccacg
    10141 ctatcaagaa gcattaaaag catataatgc agtggacttt gatgatttaa ttcgattacc
    10201 tgtcgatcta ctgaatgatc atgctgatgt gttggaatat tggcagaata aaattcgtca
    10261 tttgttggtg gatgaatatc aggattcaaa cactagccag tatcttctgg taaaaaaatt
    10321 ggcaggggtt cgtgcccact ttacggttgt gggtgatgat gatcaatcga tttatgcctg
    10381 gcgtggtgcc aaaccagaga atttggcgca gttacaaaaa gattttcctc ggttaaagat
    10441 tattaaatta gaacagaatt acagatcaac cagccgaatt ttacatgctg ccaaccatct
    10501 gatcgctcat aatcaacatt tatttgaaaa aaaattatgg agtgatttgg ggcatgggga
    10561 attattgagg gttgtcagct gtaaggatga gcaggatgag gcggaacatg tgatcgctga
    10621 tttaataagt cataaattac gtaatcggac aaattatagc gattacgcta tcttatacag
    10681 aggaaatcat caagcaagaa tatttgaaaa ggtattaaga catcacggta ttccttatca
    10741 tatcagcgga ggccagtcct ggtttgccaa gttagaggtc agggacattt ttgcctatct
    10801 taaattactt tgtaatgaag cagatgatgc cgcttttctg cgagcaatta ctacccccaa
    10861 acgaggtata ggagagagca gtctagatgc tttggggcgc tatgctcagt ccagagggat
    10921 aagtttatat cacgggtccg atcatttggc cttgtctgag tttgttgctg acaaacagag
    10981 aatgattttt catgatttca aattgtggat ggaagacatt aaaaaacgta tcagttcaga
    11041 ttcagttctg gagcatttaa ggcaaatggt ggaagatatt gggtatgaag cttatattta
    11101 tgaacaatgt gattctcccg ccaaggcaca aaaaaaaatg gataatgtat gggagctttt
    11161 agaatgggtt aatcgtttgt taaataagga acctgaacaa tcgctcatgg atgttgtgaa
    11221 taaattaata ctaatcgata tactggaaca atccgatgaa tcttcaagtg atgtagtcca
    11281 attaatgaca ttgcatgcct caaaaggttt ggaatttcct tttgtttatt tggtcgggat
    11341 ggaagaagaa ttattacccc accgagtgag tatcgatgat gaccaaattg aagaagaaag
    11401 aagattggct tatgtaggga tcacacgtgc ccagaagggg ttatgtttta ctttagcgaa
    11461 gcagagacgt cgagctggag aactgcaaga ctgtgttcca agccgatttt tggatgaatt
    11521 gccgcaagag agcctggaat ggttcggtaa aagcggggag cgttgtgaag agcaatcaaa
    11581 aaaactggcc aaatcccacc tggaaggcct gaagaatttg ttgagttaag agtgcattat
    11641 attttatctt cagtaaggag tatcattaac tgaactcctt actgcttttt aaggtactaa
    11701 tgttaatctc tatcatttgc cagaagagag attgattcgt tctggaagct cttcaatgaa
    11761 aacttgtgta cgggtttttt cccctttttg gttaaatagt ctgtgttgat tgtagtctgg
    11821 ttgcttttta gcctgctcca gagtgtcact caatgtttct cctttttcag gatggcgtat
    11881 tttttctttt aaatgattga gggcgatgac ttttttatcg gctaattcgc gaccgataac
    11941 gatgtctttg agatgctttc tttgaaattt gtcgatttga gcaacagcat gttccacttg
    12001 ccattcttgc gatgggcgtt tggcatgagt acttaattca acattgaact ctttggctac
    12061 tccccattgt ttattccatg cttctccaag acttaacaca taaggttttt tgccagaagg
    12121 caaattctta ctatgattct cttgaggagg atttagctta ctggcagaac aatcccagag
    12181 tgctgccaag ccatatatag gtgaagcaat ggtttttaat atccaggtag gaatatccat
    12241 attatgatct tgatcggagt caggagccag cctttccttt aggagttttt caaattggtg
    12301 atgatcttgg tgtccaagtt cttcatcctg ttcgtcttca tgggtatgtc caataaaata
    12361 gtgggcatct tcaaagcctt cgctaataat ggcaacgagt gcggaaagaa tctgcggtac
    12421 tccaggcata cgatccgatg tgactgcaac gctgatcaaa tgccccagaa acaaaagaat
    12481 gcgtagtggg gtaatggtta attttaaaag aatgcgaaac gggttaacta tttgtaacca
    12541 attttcagtt tcacgtaagt gctgatatcc attgcttatt gcttcgtaaa ttttttggaa
    12601 gacgttctta ttactacgag ttgcttcatc gaccatatcc agtgattcag ccgtgttttg
    12661 tatgttaaag actatagcag aggcgccagt tataatggga ttgataattc ccataataaa
    12721 acttggcatt tttttcatcc agtcaaataa agggcgggca tttgtagcaa tcgtccacca
    12781 tgttccagca gtacagatgg ttagggctac tgccaatcca acaagaaaaa cagcggtgga
    12841 tgctataaat acattgcgta cagttaaacc ttggcttaag tcatcgcgaa ttttattata
    12901 ccatttgaca accgtgttat tgttgatcat gtcggtaacg gcattgtagg tcagcattcc
    12961 ataagcggct cctgcgacga tagccatggg aaggatgata atgggccaaa atgtaaatgg
    13021 aatggcggca aaaaacggaa ttattgtaaa ggcttcaaca ataagatagg tgcttccaag
    13081 ccccatgaaa aggccggcaa ctacactaaa tcctttgaca atatgaaatt gaaaatgacg
    13141 cttttcccat cgttttatcc attcgtcttg atcatttaaa gcaagccagt caagtagttc
    13201 cctggcgtat ttggatgtgt ttggagatgg attgttatta tctgaaaaca attgcagggc
    13261 aaaccacttt tccatgtctg atagtgtctt ttcaatttgc cgctttcttt ttttactgtc
    13321 ttgatttagt tctttgtgtc caaattcagc caataatttt aactgtcttt tataatcttt
    13381 gaaaaattgc gggcaatttt cttcgttagt gtttggaaaa tgattcagca aatactcttt
    13441 cgcaaagtga ttcttaagat agttaaattt aaaaagctta ttaagagcgc cttttatgtt
    13501 ttgcaaataa atttcaccct cataagctac tgataacgca aaaccagcaa aagccaaggg
    13561 taacacaggc caaagagcat acatgcctcc aaagcttaaa aagccgagta tcaggcttgc
    13621 tccaacagtt aataacaaaa gaaacaagta ataagggatt ttttggggct taataggtat
    13681 attttttata ttcattgtag tatatttgct cattgatgcg taatgcagaa ctttatacca
    13741 aatgaaaatg attatcaata gcattaatat taaaaaagta ttttttgtct ttgtttcaat
    13801 tcaacaataa agtttgtttg caatattcgt ttcatcaggt agtatcaaaa acgatttaaa
    13861 taacttagga gaggatatgc gttttaacca atggatgacc tgtatcacat caggattaat
    13921 actcgccagt aattcaagtt ttgcaacgac gtcgcctgtt catgagcaat tgcaagtccc
    13981 acaatgcctt gctgcaaaaa taaccgtacc ccataagatt ttggccgaga ataaagaatt
    14041 caaaattatt gatgtacatt catctgatgt agagactttg actattctgg ccgataaagt
    14101 cagttgcggc cattttgtga atgtgagtca taaattaacg ggaaccttgg cggcaaacca
    14161 acagcaatct gctcaaaagt tactgcaaaa aaagctcgtt aagccgttag gtgtatccaa
    14221 attacataaa gacgtttatg aaatcaagca tgaagaagaa gtcaatgcag cattaaaaga
    14281 aattgtctct gataacatat ggcaaactct gactcatatg acttcctatt ataatcgctc
    14341 tgcaacaaaa gatacgggtg ttgaaactgc caattggtta aaattgaaat ttgaacagat
    14401 ggccgttgag tatggacgaa ctgatacctc gacctttttt gtaaaaacag ggtggtataa
    14461 acaaccttcc ctggttaccg tgatcggcaa agacatcaag gcccccgcta ttgttattgg
    14521 tgctcatatg gatactcttg atggtcgtat gcctggagct ggtgatgatg gaagtggatc
    14581 atcgagcatt atggaagccg cacgggttat tttgtcttct aaaacaacat ttaagcgccc
    14641 catctatttt atttggtatg ccgctgaaga aagaggtttg gtcggttcac aacacgtagt
    14701 ccaacacttt caggaacaat caattccagt gaaggctgtg gtacaatttg acatgacagg
    14761 ttatcgaaat gatgccaatg atccaactat gtgggtattt actgattaca cagatagaga
    14821 tttaagtaat tatttggcca aattaattga tcattatatt catgtaccag tagattattc
    14881 acgatgtggt tatggctgca gtgatcatgc ttcctggaat gaagaagata ttcctgctgc
    14941 attcccatgt gaaaccagtt ttgctgatca caacccctat atccatactt cttctgataa
    15001 aatggattta ttgaacctgg aacatatgac caatttctcc aaactcgcgg tagctttcgc
    15061 tattgaactg gcttctgaat aattcttatg cttccctgga tagttcattc aggggagtaa
    15121 ttttgttttg aatgcgtacc attatctcct ctttttacta attaaagcag tgtttacccg
    15181 ttgctgaata aaaaatgtct tatattttcc acccttaatt ctgtcttaat aattgacata
    15241 tataattgtt actggttaat ttgatttgtt atttatcatg agcaagataa ttgattcttt
    15301 aaaaaattct gatgtgcctc atttgtatct gttaaatatt ggattaacaa gagaggaata
    15361 ttacgatacc tcaaaaatga gccgtgatga aaagcgacaa ttggttaata acataataat
    15421 gaaagcttct catgaagaaa ttttgaaaat tattaatgac ttaatggcgt tggaactttc
    15481 aatagaatca aatgatccaa ttcgcactgg taatcgtctt a
//
LOCUS       DATNSN010000075        15461 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.075, whole genome shotgun sequence.
ACCESSION   DATNSN010000075 DATNSN010000000
VERSION     DATNSN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15461
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.075"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..110
                     /locus_tag="VAH76_001829"
     CDS             <1..110
                     /locus_tag="VAH76_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1386443.1"
                     /translation="VELLAILSEEYQLEVSEEDAMNFHTYKDLYDFTQA"
     gene            complement(429..1439)
                     /locus_tag="VAH76_001830"
     CDS             complement(429..1439)
                     /locus_tag="VAH76_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO1386444.1"
                     /translation="MNFFRCEKPIYIKGPFVALPERVMSNQDVLNWMNSTQNPAVIGF
                     STGIKNRHWVNEDQACSDLAVRAAEHLFMEKPREKHKVNQVILATISGDYPSPPSSPL
                     VQYRLGLQNAGAFDIGAACAGFVVGLHTSAALAQTNDGSVLLIASEIRSKFLNKNNFA
                     TSVLFGDGAAACCVSQDKEEADFRFIASALFADGEVYDAVSTPAGGSRLPAAVCNDNE
                     QFYITIKESTALFVKAVHGMADSAKDFLKELNLTISDIQWLVPHQGNKNLVLSVAKQL
                     GFPEEKTIKTVEETGNTSGSSVGIALDRLRSDGKIKSGEKVLLVAAGGGGIAACSLLE
                     VI"
     gene            complement(1436..2188)
                     /locus_tag="VAH76_001831"
     CDS             complement(1436..2188)
                     /locus_tag="VAH76_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO1386445.1"
                     /translation="MNILITGGSSDIAQAIAKRRSELGDKIIITCSNDTSLNETLAIY
                     RQQNIDVTGFIYNFADPQACENDLELILREPLDGIILNAFTRVTQLRKLHALPYHAVR
                     TYLDNNLNGNIWLIHRLLPALLKNKFGRLVLISSLSAIAGTSRYPVYCAAKAALEGLF
                     LNLAVDYSANNILSNIVRVGLIKTSRTEQFWKNPAYQEKVMRIIPQGTMGEPTQVAEA
                     IDPLLSKTSFMTGSVVTVSGGLPLMRSEGLLS"
     gene            complement(2185..2418)
                     /locus_tag="VAH76_001832"
     CDS             complement(2185..2418)
                     /locus_tag="VAH76_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1386446.1"
                     /translation="MLSNRIEQVLERIGLTNLPENKQAKLEQGGLDSLMLALLIIELE
                     REFEIKIPVIPLEKEHYESINTIEQYLIELGAK"
     gene            complement(2408..3787)
                     /locus_tag="VAH76_001833"
     CDS             complement(2408..3787)
                     /locus_tag="VAH76_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEO1386447.1"
                     /translation="MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTI
                     IVLHATPEIGFIIQLLACLETGRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQE
                     ICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLF
                     LPLSYSFGLLGQLLPGLMFGLSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAI
                     AKRYPDSAAKINAIVSAGAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKD
                     PLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEI
                     LPNGLVAIKGRRDSLVNIGGEKVNLSEIEQTICQIDVIKEAIVIPQEDKIYGTRLIVC
                     IEKKSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIGGL
                     DNKEKNNAK"
     gene            complement(3792..5537)
                     /locus_tag="VAH76_001834"
     CDS             complement(3792..5537)
                     /locus_tag="VAH76_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEO1386448.1"
                     /translation="MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQL
                     DLHAKAIAATLQAKGAKPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLL
                     DKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPT
                     SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHD
                     MGLIGCILTPIYGGIQAIMMSPFSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRI
                     REEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKDYGFRKEAFYPCYGLAEATLLV
                     TGGTPGNPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPC
                     DFDQVGEIWVQSNSVAKGYWNQPEETKHAFAGKIKDDESSAIYLRTGDLGFLHENELY
                     VTGRIKDLIIIYGKNHYPQDIEFSLMHSPLHHVLGKCAAFVIQEEHEYKLTVMCEVKN
                     RFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCRKHLLDK
                     TLPIVATWQLNKIEE"
     gene            complement(5549..6610)
                     /locus_tag="VAH76_001835"
     CDS             complement(5549..6610)
                     /locus_tag="VAH76_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEO1386449.1"
                     /translation="MNILKPDIQFEITGASRILPASGPITNLEILKNFPRTADKPQGF
                     LEKFAEKIGEEFGFHTRYWCHKPWESLDNSRELSSESLAMQAVDKLLSHSKSKEVDAF
                     LLGSTTNKRFTGSQAAAVLGNMKLNAPAYDLKTGCSTSLSTLHFAYALMALGYQKILV
                     CCSETLSKVIDPENEKTWIGLADGAASLLLEKKKAGSFTIEKSFFSTDGQYVNAFTTQ
                     GVFPPTHEQIDSVGYHLVGDETLMKELAYSRYMQMLDNLLPTEKEKNEITWVIPHQVN
                     RKLVDQVLHENHLNNKTIIWDADTIGNIGGASILYTLARAVEEKLFDRSGKILLMSVG
                     GGLSYAGQVLNYQKASHSN"
     gene            6834..8255
                     /locus_tag="VAH76_001836"
     CDS             6834..8255
                     /locus_tag="VAH76_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl CoA carboxylase subunit alpha"
                     /protein_id="HEO1386450.1"
                     /translation="MSSQHTDKTLIKWIDNFIDEQSFLPIGSEYIPDNKFHSLPGSEV
                     ITGLAKVNQRPVAIYAHDSSVNRGYVTRQGARKIIHLMDKAEKLRIPIVAFLASPGVS
                     LEENLLSGDEYTQIIARNIRLSGIIPQFAVILAPTLGAPAYSAVLMDLLFFNKHRSYL
                     MVTSPTVVQQAIGEKVTMGELGSATLHATTTGIADFVDDSVTAQINHVKAMLGFFPLH
                     TDERPPVLPIMEPNHSIPDIPANPRMPFNMLNLIQAVVDNSNVIQYKRTYGQAMICAF
                     VHIHGFAVGVVANQSIRYSGAIDSDAAQKAAKFIRICDAYSIPILTFIDVPGFMPGKR
                     EEQKGLLQHGARFCVAMQTRVPRLSVIVRKCYGAAAFLMMQTKSQNGDLVLALETANL
                     GVMGKETTNKVQAMDGQPAQEPSQSQLTEKSQIDDSLMEAYSLGLIDEIIHAAQIRSR
                     LASHLEFLYRRMEIMPPAKHSIV"
     gene            8264..9955
                     /locus_tag="VAH76_001837"
     CDS             8264..9955
                     /locus_tag="VAH76_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEO1386451.1"
                     /translation="MTSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILAS
                     SAIEFIKQWGYLDYTIPHYLGGKLNSLDELYTLTRLLARRDITMAIMFGLAFFAALPI
                     WVAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCV
                     NFATLSEYAVVLCRTNEKGGLLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFS
                     FDKVKLSKDALIGKEQRGLEITYKIFQVSRTLCACLAIGGADTALRLALSYSLQRQLY
                     GKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTLIPEKMSLWSAIIKFLIPHIGE
                     DIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGM
                     RGKNRAEHSEKIEKIFNIRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITT
                     IRHEIEKLDQEVIRLHDQKLFDPRSLAVFRLAEKYCWIFAASCCLQFWHYNQEILSDD
                     LQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHIAG"
     gene            9962..11491
                     /locus_tag="VAH76_001838"
     CDS             9962..11491
                     /locus_tag="VAH76_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEO1386452.1"
                     /translation="MNWKRFLIAAMTKKKYQVFLNDTKHPKEARERLWNSEIVPLLKK
                     SSYWQKLLKDQSSVVLNDFGITEYEDYQEGLLAAQHSTIQPFNGEELIFWSETSGTSG
                     VRKFFPITDSFQKQFQRTMAPYIYSLTQRFPGFFKEKMLYLVAVDTHKTTPAGIPTGW
                     ISNFNYRHLPSFIKKFYAMPDEVFASHEAYNQWGPIYGLASDLSAVFAVTPMVIDAFY
                     QRCIDGFKEYLPYLLGNKPLPSYLPPLKMTRKRQRHLQTLAKADHLSFKQLWPSLEIA
                     GCWTSGLCEYPAQQLQKLLGSEIKLVDGTYSATEGWLTVPVETNSVGGILHPGAHIVE
                     FIPEGEAIEKENLLQCWELEVGKKYEVFLTTAMGFIRYRLKDIVKCTGYLNSSPKLEF
                     CYKTQLLKLETCSITGRELQSAVLATSLNIEPYWYFARNSVGNRIVLVTDDEAEISEL
                     SLKKLHEQLIHISPTYAHSVETEEVLPVTLLQLPKEKLLAGMHGQTKPKFISQEVIAE
                     R"
     gene            11700..12935
                     /locus_tag="VAH76_001839"
     CDS             11700..12935
                     /locus_tag="VAH76_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386453.1"
                     /translation="MIDEKNYFLKLDFARQRVIDLPAGEFKYYLYILLESIKSQNAPS
                     RHEFLAVLLKLLDTLHKLKELKTPNFLDNQCPSSQLLKELALHLCELQRVAKTHHFAA
                     QVMIILLHIGGIISAVALSVVGGLLGGILGFVNGLCEYKPVIGFLVGTFTGTVLGGLF
                     GFRAPKKLFKNEVQRQIKFGLDGLGESLDNVNQEYSLSMFSPNKIKPFSFYLGQVEGE
                     IKQLFNDDSEYEEFLNHEIDYEINSFLASFIGESLLHGCVGHHAYIKVNIKNREYLIE
                     FTPQPTDTKEAPAQSEMRTVSGKKIVEMLAYHKKLQETNAPTASFILTKMKPGDNDCF
                     SYVNKILIGTNQQGATIKRFANMGFIGSLLGVGIEALSPFKPDFFQTTNSVRTNKEKI
                     PGHSNDLSMNSLLPQLKHG"
     gene            complement(12986..13963)
                     /locus_tag="VAH76_001840"
     CDS             complement(12986..13963)
                     /locus_tag="VAH76_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386454.1"
                     /translation="MAKNAVLAAFVLGSNVAFSGAMGPVCTPGSVNVPCPSKAWDVGI
                     QALYLKALYNNNTFSTVNIGSGSGVRIYDELDNDFEWGFRLEGSYHFSTGNDLNLNWI
                     HWRDDNKLRLAPPSGRLALQPNASVTERYYGSVEFDAVNLEFGQHVDFGQYKNIRFHA
                     GVQYVDINSETRQDEIVANAIDDLAVLNGTTSLTSTHSFSGAGPRIGADMSYDIGKGF
                     AIYGNGAAAILVGDSKYSNNAPFNQVTRPVRSVYTTLVPELEAKLGGKYTYQMTQGNL
                     IFDVGYMVVNYFDALHRSDLNLVPVVEYRDNINFGLHGPYAGVKWIGYM"
     gene            14216..15343
                     /locus_tag="VAH76_001841"
     CDS             14216..15343
                     /locus_tag="VAH76_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 repeat protein"
                     /protein_id="HEO1386455.1"
                     /translation="MDMKKYILSLSLLTVLNSSAIATTQEGEEAYNKGYYEKAFILLS
                     PEADKGDAKAQYLLGKMYYNAQGVTYNPEKTEQLLLASANQGNVDAQVLLAGFYWYLN
                     TPDGYKKAFEWYQKAADQNNADGQYGLGYMYDTGTGVPQNSDTAMVWYKKAAEQGNSN
                     AALAIGYNYDTGTGVKKDKTQALNWYAKAADLGNASAQYNLGLMYEQGDGVPKDYQKA
                     AEYFEKAANQDHAKSQLELGYLYDSGKLGKSDLQKAAFWYQKSADLGNANAQFNLADM
                     YFYGDGVGKSLEQSVYWMQKAAEQGYGKAQNQLGVYYRDGIGVAADPIKAYAWFTAAK
                     NNGFEKAASNASDLEKSMNPEDLSKARILGQQYTDNYKAKK"
BASE COUNT         4525 a         2914 c         3231 g         4791 t
ORIGIN      
        1 tcgttgagct tttggctatc ctcagtgaag aatatcaatt agaggtcagt gaagaagatg
       61 cgatgaattt tcatacttat aaagatttat atgattttac gcaagcctga tagttaccgt
      121 ttattccatt ttctgtagta aggttggtgt atttcgatca acgactgtca agttaagaag
      181 gtggcgaatt ctaatttaac aatttgaatt taaaataaaa acagataagg tgctttttga
      241 taatttttcg attctcgctt tgtcccttat ccgttcaggc gaggaagcgc ttgagcgctg
      301 tctcgaagcc atgttggtct tgataaaggc ttcgagacgg agtaaacgcc tcctcagcct
      361 gaacggatag tgtaattagt tttagcttga cgcctatggg taaaatgcct caacaaacaa
      421 tgatcaaatc agataacttc caataaagaa caggctgcaa ttcctcctcc acctgctgcc
      481 accaaaagta ccttttctcc agattttatt ttgccatccg acctcagtcg atccaaagcg
      541 atgcctacac tggatcctga agtatttcct gtttcttcaa cagtttttat cgttttttct
      601 tcaggaaaac ctaattgttt tgctaccgac aaaaccagat ttttatttcc ctgatgagga
      661 acaagccatt ggatatcaga gattgtcaga tttaattctt ttagaaaatc tttggcgcta
      721 tctgccattc catgcactgc ttttacaaat aaagcagtac tttcctttat ggtaatgtaa
      781 aattgctcat tatcattaca aactgcggcg ggcaagcggg aacctcctgc cggagttgag
      841 acggcatcat atacttcacc gtcagcaaac aacgcagacg ctataaaacg aaagtctgct
      901 tcttctttat cttgagaaac acaacatgct gctgccccat caccgaataa aacactggtt
      961 gcaaaattgt ttttatttaa aaatttggag cgaatttcgc tggctatgag taataccgaa
     1021 ccatcattgg tctgtgctaa agcagcactt gtatgcaaac cgacaacaaa tccggcacag
     1081 gcagctccta tatcaaaagc tccggcgttt tgtaggccaa gtctgtactg tacgagaggg
     1141 ctgcttggcg gggaggggta atcccctgag atagtggcca ggatcacttg gttcacttta
     1201 tgtttttccc tgggtttttc cataaacaaa tgttctgccg ctcgcacagc cagatcgcta
     1261 caagcttgat cctcatttac ccaatgccgg tttttaattc cagttgaaaa tccaatcacg
     1321 gctggatttt gggtactgtt catccaattg agaacatctt ggttgctcat gactctctcg
     1381 ggaagcgcaa caaaagggcc ttttatatag atgggttttt cacaacggaa aaaattcatg
     1441 acaacaatcc ttctgaacgc attaaaggca gaccaccact gactgttaca actgatcccg
     1501 tcataaacga ggtttttgac aaaagtggat caatggcttc cgcaacttga gttggttcac
     1561 ccatagtgcc ctgtggaata attcgcatta ctttttcttg ataggctgga tttttccaaa
     1621 attgctctgt tctggaggtt ttaattaagc ctacgcgtac tatattagac aagatattgt
     1681 tagcactata atcaacagcc aaatttaaaa ataatccttc cagagccgct tttgccgcac
     1741 aataaacagg gtatcggctg gttccagcta tcgccgaaag acttgaaatg agcacgagtc
     1801 gcccgaattt attttttagc aaggcaggta ataaacgatg aataagccaa atgttgccat
     1861 tgagattgtt atccaggtaa gtacgaacag cgtgataggg caatgcatgt aatttacgca
     1921 gttgggtgac ccgagtgaac gcatttaata tgatgccatc aagtggttct cttaaaatca
     1981 gttccaaatc gttttcacaa gcttgaggat ctgcaaaatt ataaatgaaa cccgtcacgt
     2041 caatgttttg ttggcggtat attgcaagtg tttcattaag actggtatcg ttagaacagg
     2101 taatgatgat cttatcgcct aattcactgc gtcgtttcgc tatggcttgg gcaatatcag
     2161 aggagccgcc agtaatcagg atattcattt tgcccccagc tcaatgaggt attgttcaat
     2221 agtattgatc gattcgtaat gctctttctc cagtggaatg acagggattt taatttcaaa
     2281 ttcacgttcc aactcaatga ttaacaaggc cagcatcaaa ctatccaagc ccccttgctc
     2341 aagttttgcc tgtttgtttt caggaagatt ggtaaggcca attcgttcca gtacttgttc
     2401 aattcgatta cttagcatta tttttttcct tattatctaa accgccaata ttcaaaacca
     2461 acgcttttct atcaagcttc ccatgttgat ttctgggtag ggatgacatg agctgtaatt
     2521 gaatgggcaa tttcctgggt agtagatgcg tttgtatttt ttctattaaa ttttgttcag
     2581 aaatggaaga aggcagcgat tttttttcga tacagacaat taatcgtgtc ccatatatct
     2641 tgtcttcctg agggataaca atggcctctt ttattacatc aatttggcaa attgtttgct
     2701 cgatctcaga gaggttaacc ttttctcctc caatattaac aagactgtct cgacggcctt
     2761 tgattgcaac taacccattg ggtaatattt ctgcaatatc tcctgttgat aaccagccat
     2821 tttgtatttt agtactatct ttttcaccaa gataacccag catcatttga gtgccgctaa
     2881 ttaataattc atgttcagca gaaagtttga ccttccaatc gccaacagga taacctgcgt
     2941 aattttctgt gaataaagga tccttactgg aataagtcag cactctagga gacgcttcag
     3001 ttaagccgta attgttatag atgatagcgt taggaaatat ttgtttcaaa tccgctctga
     3061 gtgtagtgga taaaggagcg ccagcagaaa caatcgcgtt aattttggct gcgctatcag
     3121 ggtatcgttt tgctatggca ttgatagcta cccagtgaga aggtacgcca ctccacattt
     3181 gaggaacggt accttgctcc attagcattt taatgtctgt aaaatgagag attagtcggg
     3241 tgctaaggcc aaacattaaa ccagggagca attggcccag aaggccaaaa gaataggata
     3301 aaggtaaaaa gagcaattga tcttgaactt ttttaaattc cagcgccttt atgaccgcat
     3361 ggcagttggc tattatgttg atgcttgata gttgaactgc ttttactgat cctgtgcttc
     3421 ctgaggtgaa gagaataagt gctgtctggg gatggggctg gatcgtttta ttttcacata
     3481 tttcctgcaa ttccccttct tcatttatca ttacggcatt acccaaaaga gacagtcgtc
     3541 tttgcttttc ttcctctgaa atggaattgg ggaaaagggc aatggggcga ccagtttcca
     3601 aacaggctaa tagttgaata ataaatccaa tctcaggtgt tgcatggagg acaatgatgg
     3661 tttttggaag tttcaggagt tgctttgaat ttgctaaaac ttcatgcgtt aattcatcaa
     3721 aagttattgc tcgattgtcc tgggtaattg ctactttgtt tggaacatct gatgccaaaa
     3781 acagcatgat tttattcctc aattttattg agttgccagg tagctactat tggcagagtt
     3841 ttatccaaaa gatgttttcg gcaaaaattc ctgcgaattt ttccgctggt agtatgtggc
     3901 attgctttaa gaggaatcag gactatagta tgtacctcca attggtggtt ttcgtaaaca
     3961 agctcaaaaa tctcattgaa taaattgtct tgagctacgt catccatgaa tcgatttttt
     4021 acttcacaca tcacagtcag tttatattca tgctcctcct gaatcacaaa agcagcgcat
     4081 ttgcccaata cgtgatggag aggagaatgc atcaggctga actctatgtc ctgagggtaa
     4141 tgatttttac cataaataat aattaagtct ttaatgcgtc cagtaacgta tagctcattt
     4201 tcatggagaa agcccaagtc cccagttctt aaatagattg cgctactctc atcgtcttta
     4261 atttttcctg cgaacgcatg cttcgtttct tcgggttgat tccaatatcc ttttgcgaca
     4321 ctgttacttt gtacccaaat ttcaccaacc tggtcaaaat cacatgggat caaggtatca
     4381 ggatctataa ttttaactgc ttgaatggga ttaccactgc tgactagctt gtaactgccc
     4441 ggactgttat cgtctgcaaa atgcacgcga tgatcctgaa attgttcttt ggccagagtt
     4501 aacgttttgt atggatttcc tggtgttcct ccggtcacta acaaagtagc ctcggccagg
     4561 ccatagcatg ggtagaaggc ttctttacga aatccatagt ctttaaatgc ctgataaaaa
     4621 tgttccatgg tttctgctcg cactggctca gcgccattga atgcggtcac ccatgaactt
     4681 aaatccagcc cttctttttt ttcttccctg atccgcttga cacaataatc gtaagcgaaa
     4741 ttggggctgc cactgatagt ggctctgtat ttggtaatat gttttaacca ggaaagcggg
     4801 ttttgtaaaa atgagaaagg ggacatcatg attgcctgaa ttccaccata gatgggtgta
     4861 agaatgcagc caatcaaacc catatcatga tgtgggggca gccagctgaa aataatggtt
     4921 tcatcattca tatgaaaaga ggtaaaaatt ttacccagat tatccagcaa attatggtgg
     4981 cttaccatca ctcctttagg gtgcatggtt gagccggaag tatattgtag aaacgcaatg
     5041 tcattgctct taatggaggt tggttgccaa ctactacttc tgtttaactc aatgctctca
     5101 agcgcaatag caggaatttt caggaattgg ggatttgtat ttaattcgtc tgcggtgaat
     5161 tttttgatgt gatccgcaat cattagtact atgaccggtt ttgagttggt aacaatgcgt
     5221 tgtgctttat ccaataattt ttcttgagcc ggtgggtaaa tgggtacagc aatgcagccg
     5281 gcataaaggc agcccaaaaa tgcctggata aggggcaatc ctggtgcaaa taataacaag
     5341 accctatccc caggttttgc tccttttgct tgcaaagttg ccgcaattgc tttggcgtgt
     5401 agatccagct gctcataggt catcgtctct tctagttctt tgttcagaaa agtacagctt
     5461 ttctttttag ggctatgcaa agcccgtaac ctgacgacgt ccaccagaga ctggcactgc
     5521 aaatattctt ttttcactat aaaacacctt aattgctgtg tgatgctttt tgatagttta
     5581 ggacttgtcc cgcataggat agaccaccac caacactcat caggagaatt tttcctgatc
     5641 tgtcaaacaa tttttcctca actgctcttg ctaatgtata aagaatcgat gcgccaccta
     5701 tattgcctat ggtatcagca tcccagatta ttgttttgtt gtttaaatga ttttcatgaa
     5761 gtacttggtc tactaatttt cgattgactt gatgtgggat tacccatgtt atctcgtttt
     5821 tttccttttc tgttggcaat aaattatcaa gcatttgcat gtatcgggaa taggctaact
     5881 ctttcattag ggtttcatca cccaccagat gataaccaac cgaatcgatt tgttcatgag
     5941 ttggcgggaa taccccttga gtcgtaaatg cgttgacgta ttgaccgtcg gtagaaaaaa
     6001 aactcttttc aatggtaaaa gaccccgcct tctttttttc caaaagtaag gaggcagctc
     6061 catcagccaa acctatccag gttttttcat tttcagggtc aatcactttg gatagcgttt
     6121 cagagcaaca aaccaatatt ttttgataac ccaaagccat caatgcgtag gcaaaatgca
     6181 aggttgataa agaagtggag caaccggttt ttaaatcgta agcgggcgca tttaatttca
     6241 tgtttccaag tacagcagct gcttgagagc cagtaaagcg tttattagtt gtggaaccca
     6301 agagaaatgc gtcgacctct tttgacttac tgtggcttaa cagtttatct actgcttgca
     6361 tagctagtga ttcagaagac agttctcttg aattatcgag tgattcccag ggtttgtggc
     6421 accagtatcg agtatgaaag ccaaattctt cgcctatttt ttctgcgaat ttttcgagaa
     6481 aaccttgcgg tttatcagca gttctcggaa aattctttaa aatttccagg tttgtgatag
     6541 ggccagatgc aggcagaatt cttgatgcgc cagtaatttc aaattgtata tcaggtttca
     6601 aaatattcat aaagtggcct tatcttgtct gggtaagtga cactattgtc attgaaatta
     6661 cactctttct attctgccag atcaagaatt tttatctttt tcgtaaaaaa gctctaaact
     6721 tatacaatta agagtagcct attttgggga ttaagttatt atgacttttc atcaaattgc
     6781 caaattagag agtctcgggc tccagggttt gaatattgca agaggaaagt ggcatgtctt
     6841 cccaacatac agataagacg ttaattaaat ggatagataa ttttattgat gaacaaagct
     6901 ttttaccaat tggttctgaa tatattccgg ataataaatt tcattccctg cctggctctg
     6961 aggtgattac cggattagcc aaagtgaatc aaaggccagt agcgatttat gctcatgatt
     7021 cttcagtcaa caggggatat gttacccgac aaggcgctcg aaagattatt catttaatgg
     7081 ataaagcgga aaaattacgc attcccatcg ttgccttttt agcatctccc ggggtttctc
     7141 tcgaagaaaa tttattaagt ggtgacgaat acactcaaat tattgctcga aatattagac
     7201 tgtcaggaat tatcccacaa tttgcagtga tactggcacc cactcttgga gcgccggctt
     7261 attccgctgt gttaatggat ttattatttt ttaacaagca cagaagctat cttatggtga
     7321 ctagtcctac agtcgtacaa caagccattg gtgaaaaagt gactatgggc gagctgggga
     7381 gcgcaacact gcatgctacg acaacgggta ttgctgattt cgtggatgat agtgttacgg
     7441 cacaaataaa ccatgtgaaa gcgatgcttg gtttttttcc tctacatacc gatgaacgtc
     7501 cccctgtact tcctataatg gaacctaatc attccattcc tgatattcct gcaaatccca
     7561 gaatgccatt taatatgctt aatttaattc aggctgtcgt ggataattca aacgttatcc
     7621 agtacaaaag gacttatgga caggcaatga tttgtgcttt tgtgcatata cacggctttg
     7681 ctgttggggt tgtcgctaat caaagtattc gttacagcgg agccattgat tccgatgcgg
     7741 ctcaaaaagc agccaaattt ataagaatat gtgatgctta ttctataccg attttgactt
     7801 ttatcgatgt tcccggtttt atgcctggta aaagagagga gcaaaaggga ttattgcagc
     7861 atggtgcaag attttgtgtg gcgatgcaaa ccagagtgcc aaggctaagt gtcatcgtgc
     7921 gtaaatgtta tggagcggct gcttttctaa tgatgcaaac caaatcacag aatggagatt
     7981 tggttttggc tctggaaaca gcaaatcttg gtgtcatggg gaaggaaacg accaataagg
     8041 tgcaagccat ggatggtcaa ccagcacagg aaccatcaca atcccaattg acagaaaaat
     8101 ctcaaattga tgattccctc atggaagctt attctttggg cttgattgat gaaattattc
     8161 acgcagcaca aattcgcagt cgccttgcgt ctcatttgga atttttatat cgaagaatgg
     8221 aaatcatgcc tccggcaaaa cactctattg tataaggaaa ataatgactt cttatgaaaa
     8281 tttgatacaa ctgtctgaat tttttgagtc atgtctgggt aatcctttgg atgacaattc
     8341 tcctgttaat tttcaacaaa ttttgcttgc tgatgagcaa gaaatattag cttcgtcagc
     8401 aatagaattt attaaacaat gggggtattt ggactatacc atacctcatt atctaggggg
     8461 taagctgaac tccctggatg agctgtatac tttaaccagg ttgttggcca ggcgtgacat
     8521 cacgatggct attatgtttg gattggcttt ctttgctgca ttgccaatat gggtggctgg
     8581 aaactcaaag caaaagaaac atttgagtga gagccttcgc cagggaaaga taggtgcttt
     8641 ggctttgacc gaagaagagc atggaaccga tttgacttcc aataagatga atgccaagcc
     8701 aactgaagat ggctgggaaa tttcggggcg aaaatggtgt gttaattttg caaccttgag
     8761 cgagtacgct gtggtgttgt gtcggactaa tgagaagggc gggttattag gtttttcttt
     8821 gttttatgtc gataaaaaac tagctgaaaa gggtatttct cctacaccta agcttccgac
     8881 tcatggggtc cgagggttag atatcagtgg tttttctttt gataaagtga aactgtctaa
     8941 agacgcatta atagggaagg aacaacgtgg tttggaaata acctataaga tattccaggt
     9001 ttcgcgaacc ttatgtgcct gcttggcgat cgggggtgct gatactgctt tgaggctggc
     9061 attatcatac agcttgcaaa gacaactgta tggcaaatca gtgtttgaaa tacccgcagt
     9121 aaaacaacgg ctgggcgaat tgtttactct tttattaatc gcggattgca ctgcccaggt
     9181 tatggtccgc gcctgtacgt taataccgga aaaaatgagt ctttggtcag caattatcaa
     9241 atttcttatt ccgcatattg gagaagatat agcagaacag tgtgccatta ttctaggtgc
     9301 cagggcttat ctgagaacca cgcaatgggc tatttttcag aaaataagac gtgatattca
     9361 agtagttggt ttgtttgatg gaagtactca agttaattta tcccttattg cgggtaattt
     9421 gttgccccag gcaggcatgc ggggtaaaaa tcgggcagag cattccgaaa aaattgaaaa
     9481 aatttttaat attcgtttgt cttgtcctcc ttttaaggag gatagactcg gtttgtttac
     9541 tcatgaagaa gatgatgtat tggctggtat cctgtcgtta aactcaattc caatcaatcc
     9601 gttgatcact acaatacgtc atgagattga aaaattggat caagaagtca tccgtttaca
     9661 tgatcagaaa ttatttgacc cacgcagtct agccgttttt cgtttggcag aaaaatattg
     9721 ctggattttt gctgccagtt gttgtttgca gttctggcac tataatcagg agatactttc
     9781 tgatgatttg caaaatacag attggctgga tttagcgatg cagttaattc ttaagcgatt
     9841 gaattcaggt tcgatgattg atagcgtgtt gcaagaagct atgagtgagc gtttgtattc
     9901 tttttatcag gaaaaacagc tgttttctat tatgccaata catattgcgg ggtaatgagg
     9961 gatgaattgg aagagatttt taattgctgc catgacaaag aaaaaatacc aggttttttt
    10021 gaatgatacc aagcatccta aagaagcgag agaacgatta tggaattcag aaattgtccc
    10081 gttgctgaaa aaatcatctt attggcagaa actattaaaa gatcaatctt ctgtagtcct
    10141 taatgatttt ggaattacag aatatgaaga ttatcaggaa ggattgttgg cagcccagca
    10201 ttctactatc caacctttta atggtgagga attgattttt tggtcagaaa cttcaggaac
    10261 atcaggagtt agaaagtttt tccctataac agattcattc cagaaacaat ttcagcgtac
    10321 aatggccccg tatatttaca gtctaactca gcgtttcccc ggctttttta aggaaaaaat
    10381 gttgtatctt gtcgcagtag atactcataa aacgactcct gccggaattc ctacgggatg
    10441 gattagtaat tttaattatc ggcatttgcc ttcctttatt aaaaaatttt atgccatgcc
    10501 tgatgaggtt tttgccagcc acgaggctta caatcagtgg ggccccatct acggtttggc
    10561 ttcagattta agcgctgtat ttgctgtgac accgatggta attgatgctt tttatcaacg
    10621 ctgtatcgac ggttttaaag aatatcttcc ttatttattg gggaataaac ctcttccatc
    10681 gtatcttcca cctctcaaga tgacacggaa acgacaaaga catttgcaga ctttagccaa
    10741 ggctgaccat ctctcattca aacaactgtg gccatctctg gaaatagcag ggtgctggac
    10801 ctctggtctt tgtgagtatc ctgctcagca attacaaaaa ttgctgggtt ctgagattaa
    10861 attggtagac ggtacttatt cggcaaccga ggggtggcta acggttcctg tagaaactaa
    10921 cagcgtgggt ggaattttac atccaggtgc ccatattgtg gaatttattc cagagggtga
    10981 ggcaattgaa aaagagaatc ttttacaatg ttgggagttg gaagtaggga agaaatacga
    11041 agtatttttg acaacagcta tgggatttat tcgctacagg ctcaaagata ttgtgaaatg
    11101 taccggatat ttgaatagtt caccaaagct tgagttttgt tataaaactc aactcttgaa
    11161 attggaaacc tgctcaatta caggtagaga actgcaaagt gcggtgctgg caacttcgct
    11221 caatatagag ccttattggt attttgcgcg caatagtgtt gggaatcgta ttgttttggt
    11281 gactgatgat gaggcagaga tttcagagct atcactgaaa aaattgcatg aacaattaat
    11341 tcatattagt cctacttatg ctcatagtgt ggaaaccgaa gaagttttac ctgtgacctt
    11401 gctgcaattg cccaaggaaa aattattagc tggcatgcat gggcaaacca aaccgaaatt
    11461 tatctctcag gaggttattg ctgagagatg aagcatccgc tgacaaattc atttcttgcc
    11521 aatcgtatca atatagatag attttggcct attttaattg gctgtgatgg atagttatca
    11581 ataatggcaa tgagcgttgt tttctgaatt attgccccat gattgatcaa atagccttat
    11641 tatagtatca ttttgggcca tgtgttttcc gttatgggtc cctgctggtt tgttatttca
    11701 tgatagatga aaaaaattat ttcttaaagt tagattttgc ccgacagcgt gttatcgact
    11761 tgcctgcagg agagtttaaa tattatctat acattctgtt agagtcgata aaatcccaaa
    11821 atgcaccatc caggcacgaa tttttagcag tactgctaaa attgctggat accttgcata
    11881 aactaaaaga attaaaaact cccaattttt tagataatca atgcccgtca agccaattgc
    11941 tgaaagaatt agcgcttcac ctctgtgaat tgcaacgagt ggccaagact catcatttcg
    12001 ctgctcaagt gatgattatt ttactgcata ttggcggtat tatttcggct gtagctttaa
    12061 gcgtagtcgg tggattgtta ggaggaatac tgggttttgt taacggttta tgtgaataca
    12121 aaccggttat aggttttctt gttggaacat ttaccggtac agttcttggt ggactttttg
    12181 gtttcagagc acccaaaaag ctatttaaaa atgaggtaca acgacaaata aaatttggcc
    12241 ttgatggttt aggtgagagt ttggacaatg tcaatcaaga atattctctg tccatgttta
    12301 gtccaaataa aattaaacca ttttcatttt atcttggcca agtggagggc gaaattaagc
    12361 aacttttcaa tgatgatagc gagtatgagg aatttctaaa tcatgagatc gattatgaaa
    12421 ttaactcctt tctggccagt tttattggag agtccttgtt acatgggtgt gtgggacacc
    12481 atgcgtatat aaaggttaat attaagaatc gtgagtattt aatagaattt acccctcagc
    12541 caacggatac caaagaagcc ccagctcaaa gcgaaatgag aacagtaagt ggtaaaaaga
    12601 ttgtagaaat gctggcctat cataaaaaac ttcaggaaac gaatgccccg acagcgtctt
    12661 tcatattaac taaaatgaaa cccggtgata atgactgttt tagttacgtc aataaaatac
    12721 tcattggaac aaatcaacaa ggcgctacaa tcaagcgatt tgccaatatg gggttcatag
    12781 ggtctttatt aggagtgggg atagaggctt taagtccttt taaaccagat ttttttcaaa
    12841 ctacaaattc agttagaact aataaggaaa aaatacctgg ccattcgaat gatttgtcaa
    12901 tgaatagtct gttacctcaa ttgaagcatg gatgattaac caaattgagg taattcaatt
    12961 tggttaattg ataatcctgc attacttaca tataaccaat ccattttaca ccagcgtaag
    13021 gaccatgtaa tccgaaattg atattatccc tatattcaac cacagggact aaattcaagt
    13081 cacttcgatg aagtgcatca aaataattga ctaccatata acccacatca aatatcaaat
    13141 taccttgtgt catttgatag gtatatttac cacctaattt tgcttctaac tctggtacaa
    13201 gggttgtata aacagagcgg acagggcgtg ttacttggtt aaaaggagca ttgttagaat
    13261 atttactatc accaactaat attgcagcag ctccattgcc atagatagca aaacctttac
    13321 cgatgtcata ggacatatca gcaccgatac gagggcctgc tccagagaat gaatgggttg
    13381 aagttaacga cgtagttcca tttaaaacgg caagatcatc aattgcgttt gctacaattt
    13441 catcttgacg tgtctcacta ttgatatcca catattgaac gcccgcatgg aatcgaatat
    13501 ttttatattg accaaagtca acgtgttgtc caaattcaag attcaccgca tcaaattcta
    13561 cgctgccata atatctctca gtcacactgg catttggttg taatgccaat ctccccgagg
    13621 gaggagccag tcgtaattta ttatcatcac gccagtgtat ccagttaagg tttagatcat
    13681 tccctgtgct aaaatgatat gacccttcga gtctaaatcc ccactcaaaa tcattatcaa
    13741 gctcgtcgta aattcttaca ccactaccgc taccaatatt caccgtacta aaagtattat
    13801 tgttataaag tgctttcaga taaagagctt gaattccaac atcccatgct tttgaagggc
    13861 aagggacatt aacgcttcct ggagtgcata cagggcccat agcaccggaa aatgctacat
    13921 tactccctaa aacaaaagct gctaatactg cattctttgc cataaatatt ccttatattc
    13981 aatacgtctt tctttatgtc tcgcatggag aacctgcata atttgaaggt taatatcggt
    14041 gatgtcaaga ataaaaaagg ttgtgttgca atttataaag gcgatatata tgacgtgatt
    14101 tatatctaat atcccaatga aagataaaaa aagcaaaatg aaaaagcatt ttatacattg
    14161 ttgaatttgt gtcatatact ttaaagcgat agctcttaaa aataaggtat atatcatgga
    14221 catgaaaaaa tatattttat ctctttcttt actgacagtg cttaactctt cagctatcgc
    14281 tacgactcag gaaggtgagg aagcctataa caaagggtat tacgaaaaag catttatttt
    14341 gttgtctccc gaagctgata aaggagatgc caaagctcaa tatttgctgg gtaagatgta
    14401 ttataatgcc caaggtgtaa cttataatcc ggaaaaaaca gagcaattat tacttgcctc
    14461 agcgaatcaa ggcaatgtcg atgcccaggt gttgttagcg ggtttctatt ggtaccttaa
    14521 tacaccagat ggctataaaa aggcgtttga atggtatcag aaagcagctg atcaaaataa
    14581 tgcagatggt caatatggct tgggctatat gtatgataca ggaactggcg tacctcagaa
    14641 ttccgataca gccatggtat ggtataaaaa agcagcggag caaggcaact ctaatgcggc
    14701 tttggctatt ggttataatt atgataccgg aacaggggtg aaaaaggata agactcaagc
    14761 tctcaactgg tatgcaaaag cagctgactt aggtaatgcc agcgcacaat ataatcttgg
    14821 tttgatgtat gagcaagggg atggtgttcc taaagattat cagaaggcag cagaatattt
    14881 cgagaaagca gcgaatcaag atcatgccaa atcacaattg gagttaggtt atttatatga
    14941 ctctggaaaa ttaggtaaat cggatctgca aaaggccgct ttttggtatc aaaaaagcgc
    15001 agacctggga aatgctaatg cacaatttaa tttagctgat atgtattttt atggtgatgg
    15061 ggtcggaaaa agccttgagc aatccgtata ctggatgcaa aaagcggccg agcaaggtta
    15121 tggtaaggct caaaatcaat taggtgtcta ttacagagat ggtattggtg ttgctgctga
    15181 tcctataaag gcttatgcct ggtttacagc cgccaagaat aacgggtttg aaaaagcggc
    15241 tagcaatgcc agtgatcttg agaaatctat gaacccagag gatctgagta aggcgagaat
    15301 tttaggacaa caatatacgg ataattataa ggctaaaaaa taattattca ggagttacga
    15361 agagatggga gtttttcgta actccggtta ttgttacctg cttttaaaag cttcggatgc
    15421 tgcatttttt gtgggctatt cgggagtatg cgggataatt c
//
LOCUS       DATNSN010000076        15435 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.076, whole genome shotgun sequence.
ACCESSION   DATNSN010000076 DATNSN010000000
VERSION     DATNSN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15435
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.076"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(75..821)
                     /locus_tag="VAH76_001842"
     CDS             complement(75..821)
                     /locus_tag="VAH76_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEO1386456.1"
                     /translation="MMKIKVLLVAALAFLISNAAFTENLTMDYVPVDINDKESLQRGA
                     KMFMNYCSGCHSLKYMRYNRMAQDLGLTTFDGQIAEDLLKNNLIFTQANIYDPIQIAM
                     SPEDAKQWFGMVPPDLSLSARDRGPVWIYNYLKSFYSDPSRPFGANNLLMPDVAMPNV
                     LEPLIGKVILVKDKNSHANSLLVVKRGEMFEGQFDSALRDLVNFLVYVGEPAKLIRYK
                     LGVFVLLFFCIFLIAAYSLKKAYWKQLKLE"
     gene            complement(818..2032)
                     /locus_tag="VAH76_001843"
     CDS             complement(818..2032)
                     /locus_tag="VAH76_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEO1386457.1"
                     /translation="MNGLVNWLDERFPLVSTWKAHFSNYYAPKNFNFLYFFGSLALIV
                     LVNQIITGLWLTMFYTPNAEQAFSSVEFIMRDINFGWLLRYMHSTGASAFFIVIYLHM
                     FRGLLYGSYQKPRELVWLLGMFLYLLLMAEAFFGYLLPWGQMSYWGAEVITSLFGAIP
                     YIGKGLAVWLRGDYSVANPTLQRFFALHVIGIPILMIILVFLHIVALHKVGSNNPEGI
                     DIKKALDKDGKPLDGIPFHPYYTVKDFVGIIVFSILFFAVVFFAPEMGGYFLEHANFV
                     PANPMVTPDHIAPVWYLAPFYAILRAIPDKLIGVVCMAAAILLLFFLPWLDKSNVHSV
                     RYKGMYSRISITLFAFSFLLLGYLGTTPVTPVRLLLARIAAVIYFSYFILMPIYSRFE
                     KTRPVPTRIGEK"
     gene            complement(2043..2660)
                     /gene="petA"
                     /locus_tag="VAH76_001844"
     CDS             complement(2043..2660)
                     /gene="petA"
                     /locus_tag="VAH76_001844"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEO1386458.1"
                     /translation="MTDLNQDVSNHNQDEQLDEERRRFLLHTTCVLGGVGAACALTPF
                     ITSWLPSSKAQAAGAPVKVDVSRMEPGEQAIIEWRGKPVWIIKRSKDMLNQLQKEDES
                     QLRDPDSLVDQQPEYAQNKFRSINPEFLVLIGICTHLGCSPKYKPNLGELGPDWPGGF
                     FCPCHGSMFDLSGRVFKGVPAPINLEVPPYHFLDDHTIVIGEDAK"
     gene            complement(2834..3265)
                     /gene="rpsI"
                     /locus_tag="VAH76_001845"
     CDS             complement(2834..3265)
                     /gene="rpsI"
                     /locus_tag="VAH76_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO1386459.1"
                     /translation="MAEMKQYYGTGRRKSSIARVFLRPGKGDIKVNDRTLQEYFCRET
                     SCMIVMQPLETVDLLNKFDVYITVTGGGISGQAGAVRLGIARALVAYDEDGLAEGAEP
                     SPDSFRRKLRARGLLTRDSRRVERKKVGLHKARRATQYSKR"
     gene            complement(3271..3705)
                     /gene="rplM"
                     /locus_tag="VAH76_001846"
     CDS             complement(3271..3705)
                     /gene="rplM"
                     /locus_tag="VAH76_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO1386460.1"
                     /translation="MKTFSAKGNEVKRDWFVVDASEKVLGRLATEIARRLRGKHKAEY
                     TPHVDTGDYIIVTNAEKVVVTGRKFKNKMYHHHTGFPGGIKSASFEKLQDKNPTKIIE
                     LAVKGMLPKNPLGREMYRKLKVYAGSEHPHTAQQPKQLEIEE"
     gene            complement(4045..4362)
                     /locus_tag="VAH76_001847"
     CDS             complement(4045..4362)
                     /locus_tag="VAH76_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEO1386461.1"
                     /translation="MLSHYTKHVFICTNQKAPGKQCCANSGGEEFFDFMKSKLLEFDL
                     HGPGKVRVSKSGCLGRCSSGPCIVIYPEGVWYTYSSFEDIEQIIKYHLIDGEIVEPLL
                     LDR"
     gene            complement(4364..4657)
                     /locus_tag="VAH76_001848"
     CDS             complement(4364..4657)
                     /locus_tag="VAH76_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEO1386462.1"
                     /translation="MNALSKAIMAETLCDELKLNKPVAKEMVENFFEELRHALENGQH
                     VKLSGFGNFTLRDKPQRPGRNPKTGEEIPVEARRVVTFKPGLKLKTKIEKIGK"
     gene            complement(4660..7041)
                     /gene="pheT"
                     /locus_tag="VAH76_001849"
     CDS             complement(4660..7041)
                     /gene="pheT"
                     /locus_tag="VAH76_001849"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO1386463.1"
                     /translation="MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTH
                     VIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGG
                     LQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
                     DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSDACPRYCG
                     RIIRNLNLEAKTPLWMAERLRRGGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEI
                     NVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGANSAVQEHTQHVFLE
                     SAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVI
                     ESFDKKFLPGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIP
                     SHRVDLQQDADLVEEIIRLYGYDKLQAQPMQTSVQAGLISAKEKIATHVSSWFSAKGY
                     HETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYNCHRQQT
                     AVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFA
                     SLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELN
                     LESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQIERVIRNTVKEDWLKSFDVFD
                     VYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE"
     gene            complement(7299..8315)
                     /gene="pheS"
                     /locus_tag="VAH76_001850"
     CDS             complement(7299..8315)
                     /gene="pheS"
                     /locus_tag="VAH76_001850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO1386464.1"
                     /translation="MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLK
                     GLANLSAEEKPKVGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNH
                     STGSLHPVTQVKHRINDYFSRLGFDIVEGPEVETEFYNFEALNIPGHHPARAMHDTFY
                     FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQ
                     ATLAGLKGLLQDFFAYFFGRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFT
                     GWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTF
                     LRQF"
     gene            complement(8452..8811)
                     /gene="rplT"
                     /locus_tag="VAH76_001851"
     CDS             complement(8452..8811)
                     /gene="rplT"
                     /locus_tag="VAH76_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO1386465.1"
                     /translation="MPRVKRGVTAKARHKKILDQAKGYYGARSRTYRVAKQAVIKAGQ
                     YAYRDRRQKKRQFRALWITRINAQARECGLSYSRLIDGLKKASIELDRKILADMAVHD
                     KVAFAAIAEQAKAALAG"
     gene            complement(8827..9027)
                     /gene="rpmI"
                     /locus_tag="VAH76_001852"
     CDS             complement(8827..9027)
                     /gene="rpmI"
                     /locus_tag="VAH76_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO1386466.1"
                     /translation="MPKLKSHRGAAKRFRKTASGAIKRRGAYRNHILTKKSTKQKRHL
                     RVEAGTLKPCDARLAERMLHGS"
     gene            complement(9048..9584)
                     /gene="infC"
                     /locus_tag="VAH76_001853"
     CDS             complement(9048..9584)
                     /gene="infC"
                     /locus_tag="VAH76_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO1386467.1"
                     /translation="MSAPTKRENDRARINEQITVPEVRLIDVDGNQAGIVSTREALRA
                     AEESGLDLVEISPTAKPPVCRIMDYGKFLFELSKKQAEAKKKQKQIQVKELKFRPTTE
                     DGDYQVKLRNLIRFLNHGDKVKITLRFRGREMAHQELGMKILERLQADTADVAIVEQH
                     AKREGRQLLMVLSPKKTK"
     gene            complement(9609..11522)
                     /gene="thrS"
                     /locus_tag="VAH76_001854"
     CDS             complement(9609..11522)
                     /gene="thrS"
                     /locus_tag="VAH76_001854"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO1386468.1"
                     /translation="MPNVKLPDGNVKHFEAPLTIYDVAHHISPGLAKAAIAGRVDGVL
                     VDTSYLIKEDCSLIIVTEKHEDSLEIIRHSTAHLLAQAVKALFPSAQVTIGPVIEDGF
                     YYDFAFERSFTPDDLSLIEAKMHELAKANLSITRRELPRNEAIQYFKSLGEEYKAKII
                     ADIPENEALSLYRQGDFEDLCRGPHVPSTGFLKAFKLTKVAGAYWRGDSNNEMLQRIY
                     GTAWADKKSLEEYLFRLEEAEKRDHRKLGKALDLFHFQDIAPGMVFWHPKGWTIYQEL
                     EHYMRNRLVDFGYQEIRTPQLVDRSLWEKSGHWANFRDEMFVTETENRHYAVKPMSCP
                     CHVQIYNHGLKSYRDLPLRLSEFGNCHRCEPSGALHGLMRVRNMVQDDAHIFCTEDQI
                     QSEVAMMLELVQSVYKDFGFTEIKYRLALRPEKRVGSDDVWDKAETALKLAMQGRNIE
                     WVDAPGEGAFYGPKIECSLSDCLGRIWQCGTIQVDFSMPARLEASYVAEDGSKQTPVM
                     LHRAILGSFERFMGILIEHYAGKLPLWLSPVQAVVLTISEKQNEYAEKVRKTLQKRGI
                     RANFDLRNEKIGFKIREHTLQKIPYLLVVGDKEVENCQVAVRTRDGIDLGVMTIDTIC
                     DTLTQEIIRKGSI"
     gene            complement(11650..11724)
                     /locus_tag="VAH76_001855"
     tRNA            complement(11650..11724)
                     /locus_tag="VAH76_001855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11690..11692),aa:Val,seq:gac)
     gene            12037..12324
                     /locus_tag="VAH76_001856"
     CDS             12037..12324
                     /locus_tag="VAH76_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEO1386469.1"
                     /translation="MWFKIIDSHVEIAIYAKPNAKKTKLMAISDDRLHIALHAKPQEG
                     EANNELLFFISQFFKIPKTQIELIKGKSSRHKLIRLPLSESVFRFLNNPTI"
     gene            12659..13144
                     /locus_tag="VAH76_001857"
     CDS             12659..13144
                     /locus_tag="VAH76_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1386470.1"
                     /translation="MEKVNLTKQFAYRLRNAMIAAGHNSQRSPSGVCIHKLAEMTGYS
                     LQICRKYLRGEVIPDPVKLIEIASKLNVSPGWLLFGETHNDPGTSKEHITISKSLLHH
                     IFTKAACLYNRELVEDDVSDFLMDLINDISLINATEEQSKKIIDLAISSVSHFRHSQG
                     T"
     gene            complement(13852..15414)
                     /gene="wipA"
                     /locus_tag="VAH76_001858"
     CDS             complement(13852..15414)
                     /gene="wipA"
                     /locus_tag="VAH76_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipA"
                     /protein_id="HEO1386471.1"
                     /translation="MPKRLINKNIDIYNYPNELEDNLGSISLGDLHGNAIKLIHFLFR
                     HKIIKFKPEISNFHEAYQQFVTIYEQYDDMVQEYLEIRTLLQLIQIKITNTKQRILDI
                     DQKLSLPTDSQKEFSQSLLQLKKSTEANLQIAEMNKAGLEEKLSGLKTRLPSCIERFN
                     KFMTQIEINDIKTLIRLLGDEVADRGSCDYFTLRILDFLYQNQIATKIILSNHGYEFI
                     HAYEKLVIGQPFKPKGYIGDIQIKSFWGIQLLLEQSAITQEELRSLVERAYKPTLKII
                     DYSLSEDGITLYSHAPIRFDSIRMAASQLGVTYNDSTKEALAETIDQLNAQLQIYMKN
                     NMLHLLFENNEINDPTNMTDEERNASPLIYLVWNRWNESKEVENARPGKYNGYFVTYV
                     HGHDPFQSPLTYVYNLDTLCGKYSRMSEEEQINKAFQFLTENRHTNVDKTASESLLRN
                     ISRYKVLDSDEYTLKHKIPKTSPELATDILDCKIKESLIKLSLLGKPKAVSGSLSVDQ
                     NTSIPNQASVGK"
BASE COUNT         4725 a         3398 c         2637 g         4675 t
ORIGIN      
        1 gtaaccattt gagattgaac tctgtataaa aggcaaaaaa gtagcacctt ttacaaagct
       61 gaggagtaag cagtctattc caattttaat tgtttccaat aagctttctt cagtgaataa
      121 gctgctataa ggaaaataca gaagaacaaa agaacaaaaa ctcctaattt ataacgaatt
      181 agcttggcag gctcacctac ataaaccaaa aaattgacta aatcccttaa tgcgctgtca
      241 aactggcctt caaacatttc gcctcgtttt accactaata aactatttgc atgcgaattt
      301 ttatctttga ccaaaatgac ttttccaatc aagggttcca aaacattagg catagcaaca
      361 tcaggcatta gtaaattatt agcaccaaaa ggtctggaag gatcgctgta aaaacttttc
      421 aaataattat atatccaaac aggccctcta tcccgtgcac ttaaagacaa atcggggggc
      481 accataccaa accattgctt ggcatcttca ggcgacatgg caatttgaat gggatcataa
      541 atattggcct gagtaaaaat taagttattt tttaataaat cttcagcaat ctgaccatca
      601 aaagtagtta atccaagatc ctgtgccatg cggttataac gcatgtattt gagagaatgg
      661 catcccgagc aataattcat aaacatcttt gcccctcttt gcaaactctc tttatcgttt
      721 atatcaaccg gtacataatc cattgttaaa ttttctgtaa aagcagcgtt tgatattaaa
      781 aatgccaatg cagcaactaa taacaccttt attttcatca tttttcacct atccgtgtag
      841 gtactggccg ggttttctca aaacgactat aaataggcat caagataaaa taagaaaaat
      901 aaataacagc ggcaattcga gctaaaagca accttactgg tgtcaccgga gttgtgccca
      961 aatatcctaa aagcagaaaa ctaaacgcaa acaaggtaat acttattctg gaatacattc
     1021 ctttatatct tacagagtgc acattacttt tatcgagcca gggcaaaaag aaaagcaata
     1081 atatggcagc agccatgcac accaccccaa ttagcttatc tggaatagcc cttaaaatgg
     1141 cataaaaagg tgcgagatac caaacaggag caatatgatc tggagtcacc attgggttag
     1201 cgggtacaaa attagcatgc tctaaaaaat atcctcccat ttcaggggca aaaaagacaa
     1261 ccgcaaaaaa taaaattgaa aagactatga tacctacaaa atctttcaca gtgtaataag
     1321 gatggaaggg tatcccatcc aaaggctttc catccttatc cagggccttc ttgatatcaa
     1381 tcccttccgg attatttgag cctactttat gcaatgcaac aatatgcaaa aacacgagga
     1441 tgatcattaa aatgggtatt cctatgacat gcaaggcaaa aaaccgctgc aacgtcggat
     1501 ttgcaacaga gtaatctccc ctcaaccata cagcaagacc tttgccaata taaggtatcg
     1561 ctccaaataa ggaagtaatc acttcagcac cccagtaaga catttgtccc cagggcaata
     1621 aataaccaaa gaatgcttct gccattaaca agaggtataa aaacattccc agtaaccaaa
     1681 ctaactcccg tggtttctga taagaaccat aaagcaagcc tctaaacata tgcaaataga
     1741 taacaataaa aaaagcagag gctcctgtgg aatgcatata acggagtagc cagccaaaat
     1801 tgatgtcccg cataatgaat tcaactgaag aaaatgcctg ctccgcgttc ggcgtataaa
     1861 acatggttag ccataaacca gtaatgattt gattgactaa aactatcagc gctaaagatc
     1921 caaaaaaata aagaaaatta aaatttttag gcgcataata attactgaaa tgtgctttcc
     1981 aagtgctaac aagagggaaa cgttcatcta accaattgac taacccattc atgccttaat
     2041 ccttattttg catcctcacc tatcacaata gtgtggtcat ctaaaaagtg ataaggtggt
     2101 acttccaaat tgataggggc aggaacgccc ttaaatactc ggcctgaaag atcaaacatt
     2161 gacccatggc atgggcaaaa aaagccacct ggccaatcgg ggcctaactc accaagatta
     2221 ggtttgtatt ttggggaaca acccaaatga gtacaaatcc cgattaaaac caaaaactcg
     2281 ggattaatgg agcgaaactt gttttgcgca tactctggtt gttgatccac caaagaatcc
     2341 ggatccctca gttgagactc atcttctttt tgtaactggt taagcatgtc cttgcttcgt
     2401 ttaattatcc atactggttt gccacgccat tcaataatag cctgttcacc aggctccatt
     2461 ctactgacat caactttgac tggagcacct gctgcttgtg ctttagagct tggtaaccag
     2521 gaagtaataa aaggagttaa ggcgcatgca gcgcctaccc cgcctaagac acaggtagta
     2581 tgcaacaaaa aacgacgccg ctcttcatcc aattgttcgt cttgattatg attacttaca
     2641 tcctgattga gatcagtcat ttcgctcact gcttaatcat cctgattatg gtctatgaac
     2701 tggagccagt tcgcctaata taactcccgt tagttataac aaagaataac aatttttcaa
     2761 acggttggat aacttatttc ttagaaaata aaaaagcctc gtataaaacg aggcttggaa
     2821 aaaagtaata gaattagcgc ttggagtact gagtagctcg gcgtgctttg tgcagcccca
     2881 ctttctttcg ttccacacgt cttgaatcgc gagttaataa cccacgcgct cttagcttgc
     2941 gacggaacga atcgggactt ggctcagcgc cttcggccaa accatcttca tcataagcaa
     3001 ccaatgctct ggctattccc aaacgaacag ctcccgcttg gccagatatg ccaccaccag
     3061 ttacagtaat gtatacatca aacttattaa gaagatctac tgtttccaaa ggttgcatta
     3121 caatcatgca tgaagtttct cgacagaaat attcctgcaa ggttctgtcg tttactttaa
     3181 tatcaccttt tcctggacgc aaaaatacac gtgcaattga actttttcta cggccggtgc
     3241 cataatattg cttcatttca gccattatac ttattcctca atctcaagtt gcttcggttg
     3301 ctgcgcagta tgtggatgtt cgctgccagc atatactttc aacttgcgat acatctctct
     3361 acctaacggg tttttgggca acatgccttt tacagcaagc tcaataattt ttgttggatt
     3421 tttatcctgt aatttttcaa aggatgccga tttgatccct ccaggaaacc ctgtatgatg
     3481 atgatacatt ttgtttttaa acttgcgacc agtcacaaca accttttctg cattagtgac
     3541 tataatgtaa tcgcctgtat caacatgggg agtatactca gctttatgct tgccacgtaa
     3601 acgacgagca atctctgtag ctaagcgacc caaaactttt tcactggcat cgacaacaaa
     3661 ccagtcacgc ttgacttcat tgccttttgc actaaatgtc ttcattaaat aaactccgaa
     3721 ccgcctcaat tggtaatctt tctatcatgg acgggcgatt ttaaccaaaa cagaaacaac
     3781 ctacaagtaa aatggataaa aaaatatata caattaatca aatctcaata aaaacatcag
     3841 attaatgact aaatgtgaag tatttttcat cttattgacg taagttcata ctgaatcaac
     3901 agaaaacccc gatactcatt ttcatctctt tgtttcatta gaagagactg ggcctgacta
     3961 ctcactcaga attaaccaac gcttaatttt atcaaaagcc ttttcgtgcc aggaaagcac
     4021 aaaaaagttt atttctctaa ataattatct gtctagcaga agtggctcta ctatctcacc
     4081 atcaattaag tggtacttga tgatttgttc aatatcttca aaggaagaat aagtatacca
     4141 aactccttct ggataaataa cgatgcaagg accagaacta caacgtccaa ggcatcctga
     4201 tttgctgacc cggactttcc cagggccatg caaatcaaat tctaacaatt ttgatttcat
     4261 gaaatcaaaa aattcttctc cgccggaatt agcacaacat tgcttacccg gtgctttctg
     4321 atttgtgcat atgaatacat gcttggtgta atgagacaac aaactacttt cctatttttt
     4381 caattttcgt ttttaatttt aaacctggct taaaggtaac aacccttctg gcttccacag
     4441 gaatttcttc acctgtcttt ggattgcggc ctggcctttg tggtttatct ctcaaagtaa
     4501 aattaccaaa accggataat ttcacatgct gaccattttc aagagcatga cgtaattctt
     4561 caaaaaaatt ttcaaccatt tctttagcaa ctggtttatt taattttaat tcgtcacaca
     4621 atgtttctgc cattattgct ttgcttagtg cgttcacgat cattccctca aaaggattga
     4681 aaattcattt tccagtttct tgattatagc actaattgtt aaattgatct cggcatcaac
     4741 taaagttcga gtatcgtcct gtagtgtcat agccacagca atactttttt tatcttctgg
     4801 aataccttta cccatgtaca cgtcaaatac gtcaaacgat tttaaccaat cctctttaac
     4861 tgtatttctg attacgcgct caatctgcat ggcacttatc tgtctatcaa ccaggaaaga
     4921 caaatctctc cttatttgtg gatatttgga aataggctta tacagaggaa tagtcggatt
     4981 gattaatgat tcaagattca attcaaacaa aaccacatcc tggtcaagat caaaggcgtc
     5041 cgacaagcga ggatgcagca cacctatcca gcctgaatgc ttgccattaa tcacaatctg
     5101 ggctgactga ccagggtgaa gcgcatgatg agatgattga ataaattcaa catcatccag
     5161 cttcaatgaa gcaaacaagg attgcaaatc cccttttaaa tcataaaaat caaataaacg
     5221 ggcagattca ctccaattca aattgccttg ctcccccatt aacaagccag caatacatga
     5281 acgctccttt aattgccctc cgtccagatc aaaaaccacg cctatttcaa aaaatttcac
     5341 tgcggtttgt tgcctgtggc aattataaat catggatgca attaagcctg gccacattcc
     5401 cgcacgcatt tgcgataatt cagaggaaat tggattaagc agttccataa attctttttg
     5461 tgggtaaagc gcttcctgca gctcaggatc gacaaagcta taactgatgg tttcatgata
     5521 gcctttggca ctaaaccacg atgaaacgtg tgtcgcaatt ttttccttgg cactgattaa
     5581 accagcttgt actgatgttt gcattggctg agcttgtaac ttgtcatagc catataaacg
     5641 aataatttct tcaaccaaat cagcatcttg ttgcaaatcg actcgatgag aaggaatggt
     5701 gacttcaaaa aaatgattgg tttctttgat gatgacaaca cctaaacctt ccagtaaatt
     5761 tttcatttcg tttagaggga tagacagccc agttaacttt ttcacttttg tcgtatcaaa
     5821 taaaaaggaa actgtacctg gcaaaaactt tttgtcaaaa gactctatca ctggcccagg
     5881 ttcacctcct gaaatagata aaatcaattc cgttgctctt tcaagcgctt ttgattgcaa
     5941 acaaggatct actccccgtt cgaatcgttg tgacgaatca ctgaataagc catattttct
     6001 ggcaactccg gcaattgtca ccggattaaa ataggcgctt tccaggaaaa catgttgtgt
     6061 atgctcttga acggcgctgt tagctcctcc cattattcca gccatagcca gaggcttttc
     6121 tttatcagcg attaccaaca cattgtcgtt taatacaact tcttgaccat ccaataattc
     6181 taattgctcg cctgaagcac tataacgtac gttaatttca ccgttaattt ttgataaatc
     6241 aaaagcatgc atgggttgtc caagctcgag catgacataa ttcatcacat ccactacagg
     6301 gtgcaacgtt ctaatgccgc ctctacgtaa gcgctcagcc atccataatg gcgttttcgc
     6361 ttcaagattt aaattacgga taattcgacc gcaataccgt ggacatgcat cagaatgaat
     6421 taaattcact cgaagaccat catcgatggc tggtggtact gtaactattg gttgctctat
     6481 taagggtaat ttattgagaa cagccacttc ccgcgcgaca cctaaaatac taaagcaatc
     6541 tgcgcgattc ggagtcaaat caatatcaaa tacatggtca tcaagggcaa gatactcgcg
     6601 taaatccatt ccaattggtg cctcatcact cagttccatt atcccctcgg aatgttctgc
     6661 cagtcccagt tctgtcgcgg aacacaacat gccctgagac aattctccac gcaatttgga
     6721 ttccttaatt tgcaaaccgc caggcaagtg tgccccaatc atggccaggg caactctcaa
     6781 accaggcctc acattagccg caccacaaac aattttcagc ggcttgtctt tattgatatt
     6841 cacttcacat aaggttaatt tatcagcatc tggatgaggc ttggtattta agacttcagc
     6901 aacaatgaca tgagtgaatt ggccagctac aggattcact gcgtcgactt ccaagccagc
     6961 catggtcaat tgctcagcca gttcttgttc agttaatgag aagtttaccc attctcgtaa
     7021 ccataactta cttactttca tttaaagtcc tcatctctgt aactgttcat ttggctcaaa
     7081 gtttccatcc gttaattttg ctctccctat gataatagag ataaaattac tgataactat
     7141 ttgcctgtat caactacata ataaaatatt attttcagta ttcatgggaa ttatcccggg
     7201 ctacaatttg gtctaatctg tatcatttat gtaccatatg aaagacaatc ataaagaaac
     7261 ataaaaataa aatccgggac tcattatttt tataatggtt aaaactgccg taaaaaagtt
     7321 aaatcatttt caaataacat acgcaaatca tctatgccat aataaagcat tgccaatctg
     7381 tccataccca tgccaaaagc ccatccatga tactcattcg gatcaatgtt cactgcgatt
     7441 aaaacgttag ggtggaccat accacaaccc aatacctcaa gccagcctgt aaacttgcaa
     7501 gagcggcaac ccttaccatt acattgagta cattctatat ccacttctgc ggaaggttca
     7561 gtaaaaggga aataggaagg tctgaatctt aatgccaatt cccgaccaaa aaaataagcg
     7621 aagaaatctt gcaacaaccc ctttaacccg gccaaagtgg cttgcttatc gatgagtaat
     7681 ccctccactt gatgaaacat aggggtatgg gttacatccg aatcacaacg ataaactcgg
     7741 ccaggagcaa ttaaacgaaa aggtggctta cgttgctcca ttgtcctgat ttgtactgga
     7801 gatgtgtgtg tcctcagcaa gcgtccgtct ccaaaataaa acgtatcgtg catcgcacgt
     7861 gcaggatgat ggccagggat atttaatgct tcaaaattat aaaattctgt ttcaacttca
     7921 ggaccttcaa caatatcaaa tcctaaacga ctaaaataat cattaattct atgctttacc
     7981 tgagtaacag gatgtaggga acctgtggaa tgattgcggc ctggaagagt aacgtcgatt
     8041 tgctctgctg ccagcttggc taataattgc ttttctttca gctcgatcat ttttgtttca
     8101 atcaaagcac taattccctg ctttgcttga ttgaccaatt gacctacctt gggtttttcc
     8161 tctgcagaca aattagcaag tccttttagc aattcggtga gcttgccttt cttgcccaaa
     8221 aaatcaacac gaatttgttc aagagcaact atatccgtgg cttgtttgat tgcttctgaa
     8281 gcttgttctt gtatggtgtt tataagtacc aacatagttc tacccacaag taaaaaggag
     8341 gccttagcct cctcaataat ttttatttta cataccggct tttatttaag attgctattc
     8401 cgaattgaga tttttaaacg aaaggcaata acaaataaag cgggctgaaa attaaccagc
     8461 taaagctgct ttggcttgtt ctgcaatagc agcaaaggca actttatcat gaaccgccat
     8521 atcagccagg attttcctgt ccagctcaat ggaagctttt ttcaaaccat caatcaagcg
     8581 gctgtaggat aacccacatt cacgagcttg agcattgata cgagtaatcc ataaagcacg
     8641 gaattgtcgc tttttctgcc ttctgtctcg gtatgcgtat tgaccagctt taattacagc
     8701 ctgtttagca actcgatagg tacggctacg ggcaccataa taacctttgg cctggtctaa
     8761 aattttcttg tgacgcgctt ttgctgtcac accacgtttt actcttggca ttgttcaatc
     8821 tccccgttaa ctaccgtgca acatacgttc agccaaacgc gcatcgcatg gcttcaaagt
     8881 acctgcttct acacgcaaat gacgcttttg cttagtagat tttttagtga ggatatgatt
     8941 tctgtaagcg ccgcgacgtt taatcgctcc actcgctgtc tttcggaagc gtttagctgc
     9001 tccacgatgt gactttaact tcggcataac aatatactcc gcattcgtta cttggttttt
     9061 ttgggtgata ataccatcag taattgtctt ccttcacgtt ttgcatgctg ctccactata
     9121 gctacatcag ccgtgtcggc ttgcaaacgt tccaaaatct tcataccgag ttcttgatga
     9181 gccatttcac gaccgcgaaa acgcaatgtg attttgacct tatcaccatg attgaggaaa
     9241 cggatcaggt tgcgtagctt gacctgataa tctccatctt ccgtcgtagg acggaatttt
     9301 aattctttga cctgaatttg tttttgcttc ttttttgctt cagcttgttt tttactcagt
     9361 tcaaagagaa acttaccata atccataata cgacaaacag ggggtttggc tgttggagat
     9421 atttccacca aatccaatcc gctttcttct gctgccctta acgcttctcg cgttgataca
     9481 attcctgcct ggttaccatc gacatcaatt aagcgtacct caggtacagt gatctgttca
     9541 ttaattcgtg cgcgatcatt ttcacgctta gttggtgcac taatggttta atcctccagt
     9601 tcgtttattt atattgatcc tttacggata atttcttgcg ttaaggtatc acaaattgta
     9661 tctattgtca tcacacccag gtcaatgcca tctcgagtgc gaacagctac ctgacaattt
     9721 tcaacctctt tatcgccaac aaccaataaa taaggaattt tttgtaaagt atgctcgcga
     9781 attttaaatc caatcttttc atttctcaag tcaaaattgg cgcgaatccc tcttttttgc
     9841 aaagttttcc tcactttttc ggcatactca ttctgcttct cactaatagt aagcacaacc
     9901 gcttgcactg gggataacca taatggcaat ttacctgcat aatgttcgat taatattccc
     9961 ataaatcgtt cgaaagaacc caaaatggct ctatgcaaca tcaccggtgt ttgcttgctt
    10021 ccatcttcag caacatagct tgcctcaagg cgtgcaggca tagagaaatc cacttgaatc
    10081 gttccgcact gccaaattct acctaaacaa tcagaaagag agcattcaat tttaggtcca
    10141 taaaatgccc cttctccagg cgcatccacc cactcaatat ttcttccctg catagccagt
    10201 ttaagggcgg tttccgcctt atcccaaacg tcatctgaac cgactcgttt ttcagggcgc
    10261 aaagccaatc ggtattttat ttcagtaaac ccaaagtcct tatacacgga ttgcactaac
    10321 tccagcatca tagccacttc agattgaatc tgatcctcag tgcaaaaaat atgagcatca
    10381 tcctgcacca tattacgtac ccgcatcaac ccatgcagcg cacctgatgg ctcacagcga
    10441 tgacaattcc caaattcaga tagtctgaga ggaagatccc ggtaactttt caaaccatga
    10501 ttataaattt gaacgtgaca gggacagctc ataggcttta ctgcataatg tcgattctct
    10561 gtttcggtaa caaacatttc atctctaaaa ttcgcccaat gacctgattt ctcccataac
    10621 gatctgtcta ctaattgcgg tgttctaatt tcctgatagc caaaatcgac caggcgattt
    10681 cgcatgtaat gctctagctc ttgataaata gtccatcctt tggggtgcca aaaaaccatt
    10741 cccggagcaa tatcctggaa atgaaacaaa tccagtgcct tgcctaattt acgatgatca
    10801 cgtttttctg cttcttccaa acgaaacaaa tattcttcca gagatttttt atcagcccag
    10861 gcagtgccgt aaattcgctg caacatttcg ttatttgagt caccacgcca ataagcgcca
    10921 gctacttttg tcaatttaaa tgcttttaaa aacccagtgg aagggacatg tggacctcga
    10981 cacaaatctt caaaatcacc ttgcctatat agagagagcg cttcattttc gggtatatca
    11041 gcaataattt ttgccttgta ttcttcgcct aaactcttaa aatattgtat tgcttcattt
    11101 ctaggtaatt ctcgacgagt gattgaaaga ttagcctttg ccagctcatg catcttcgct
    11161 tcgattaaac tcaaatcatc gggagtaaac gagcgctcaa aagcaaaatc ataataaaac
    11221 ccgtcttcta ttacaggacc tatagtcact tgagctgatg ggaataaggc ttttacagcc
    11281 tgggctaata aatgagcggt agaatgcctg attatttcaa gactgtcttc atgtttctcg
    11341 gttacgataa tcaaagagca gtcttctttt ataagatagg atgtatccac caagacgcca
    11401 tcgacacggc ctgctatcgc tgctttagct aatccagggc taatgtgatg tgccacatca
    11461 taaatagtga gtggcgcttc aaaatgtttt acatttccat ctggtaattt aacatttggc
    11521 ataatcttat ccgtctttcc atctttcagg ttcttgttaa aaaaaaaccc tgcatggcag
    11581 ggttcaaacc tttaataatt cagtgcagta gtttaccaat tagagattct actagaaatc
    11641 caggaattat ggtaggcacg agtgggatcg aaccaccgac caccaccatg tcaaggtggt
    11701 gctctaccac tgagctacgt gcctataatt caacctgaat gaaaattggc atgaattata
    11761 cacataagat tcatggaaat gcaatcatcc aagtcatatc atttttatct aatgaaaaag
    11821 caagttaaca tgacattaaa attttggatg agcctggcct atcaatcttg ccttttaact
    11881 cttttgcaaa tgcaaccctt cccaaccaaa ccgcaaaaca ctcttgcttt ccccaaaatg
    11941 cattagtgca agaagaataa agagcatgaa aagctaattc caactctctt gggaacaagg
    12001 taaaatgata aagcctccgt tagcgtttaa aatattatgt ggtttaaaat catagattcc
    12061 catgtagaaa tagctattta tgccaaaccg aacgccaaga agacaaaact catggccatt
    12121 tctgatgaca ggctgcatat tgcgctccat gccaaaccgc aggaaggaga agcgaataat
    12181 gagcttttat tctttatatc ccaatttttc aaaattccaa aaacacaaat cgagctaatt
    12241 aaaggaaaaa gcagccgtca taagctaata agattaccct tatcagaatc tgtgttccgc
    12301 tttctaaaca atccaacaat ctagaacata tcacccttta aaacttagaa atggcccgaa
    12361 tgaaggggtt cattcaagcc atgcaatgcg aggttaaccg ctatggttaa accacttttt
    12421 ctattgattg gactaccctg tgatacttgt tccttcaagt aacatcagcg tccgatattt
    12481 ggacttaaag aaacgcgtta taacgttcca acgcccatta taatcattta acaccaaaaa
    12541 tcaactaatg atttccaata gaaacaattt gtttccatta aatggatata tttgcaggtt
    12601 taactaccct ttgctacact tttgtcactt tttaatatct taaaattatt actccaatat
    12661 ggaaaaagtt aacttgacaa aacagtttgc ttaccgcttg cgtaatgcca tgattgcagc
    12721 agggcataat tctcaacgct ctccatcagg agtatgtatt cataaattag ctgaaatgac
    12781 aggttactct ttgcaaatat gcagaaaata ccttcgtgga gaggtcattc ccgatccagt
    12841 taagctgata gaaattgcat cgaaactaaa tgtttcgcca ggatggcttc tgtttggtga
    12901 aacccataat gatcctggaa catctaagga acacatcacg ataagcaaaa gtttactcca
    12961 tcatattttt acaaaagcag cttgtctata taatcgtgag cttgtagaag atgatgtgtc
    13021 tgactttttg atggatttga tcaatgacat tagtctcatc aatgccaccg aagaacaatc
    13081 caaaaaaata attgatttgg ctatctcttc agtgagccat tttagacact cgcaaggaac
    13141 gtagagtaag aaactttcat ggaaaatagc tcaaaagaga tagtgctcca ctctcaactt
    13201 ttagagaacc ttttcgcctt taaaagtaaa gtatccaatg tctttacaga cgtattgggc
    13261 atacatgacg ttcatcatat ttccttaaca aatattaacg ggcacaatca attgctgccc
    13321 ctatcctcta ctcctgccat ggaatttaac tgatttaaca gttttctttg gcaacatgac
    13381 ctaacctata atcccaagtg atttaaatta tgtactcaag gcgattggca cacactttaa
    13441 taatataaaa cgctatggtg agctatatta gcttgatcaa attaaaatgg ttttcccatc
    13501 ggtttctccc ttgctatcaa gataaatgaa aattatgtga tttactcaat agcaagccac
    13561 aaatcttgtg cctttactcg tgagttattt tctactcatc aagaagagtt ttaacaaata
    13621 gggccatatt gctccaattt tctgcatcca ttttttagtt attatgagga ttttccttct
    13681 ccatatttaa ttttaaactc acactgaata ataaacatcc atcccgactt tgtaatgaat
    13741 tacttgattt ttaaagagct cgataatcta cttacgtacc tcaatttttt cgaagcattt
    13801 tgtgcgcatt ttatggctat ctctaaaacg cagcgcttca tagagagtca gtcatttgcc
    13861 cacagacgcc tgattaggaa tagaagtatt ctggtctaca ctcagcgaac ctgataccgc
    13921 ctttggtttc ccgagcaagc tcagttttat taaactctcc ttaattttac aatcgagaat
    13981 atctgttgcc aattcaggtg acgtcttagg gattttgtgt tttaaagtat attcatccga
    14041 atccagcact ttataccttg atatgttgcg aagaaggctt tcacttgctg ttttatccac
    14101 attggtgtgt cgattttcag tcaaaaactg gaaggctttg ttaatttgct cttcctcact
    14161 cattcttgag tattttccac acagagtatc cagattatac acataggtta atgggctttg
    14221 aaaaggatca tgcccatgaa cataggtgac aaaataacca ttgtattttc ctggtcgagc
    14281 attctctact tctttgcttt cattccagcg attccatact aaataaatta acggcgatgc
    14341 gtttctctct tcatcagtca tattggttgg atcgttaatt tcattattct caaataacaa
    14401 atgcagcatg ttgttcttca tataaatttg caattgggca ttaagttggt ctatggtttc
    14461 agccaatgcc tctttggtgg aatcattata agtgacaccc aactgagaag ctgccattcg
    14521 aatgctatca aagcgtatgg gagcatggct atacaaggta ataccgtcct cgcttagaga
    14581 ataatcgata atttttaaag ttggtttata agctctctcg actaaacttc tcaattcctc
    14641 ctgagtaatg gcactttgct ccagtaataa ttggataccc cagaatgatt taatttgtat
    14701 atccccaata taacctttgg gtttaaatgg ttgaccgatt acaagttttt cgtaagcatg
    14761 aataaactcg tacccatgat tggataaaat gatcttggtt gctatctgat tttgataaag
    14821 gaagtccagt attcttaaag tgaaataatc acaactcccc cgatcggcca cctcatcacc
    14881 cagcaatcga ataagcgttt taatatcatt aatttctatc tgcgtcatga acttattaaa
    14941 tcgctcaata catgatggca gccttgtttt caatccactt aatttttctt ccaggccagc
    15001 tttattcatt tcagctattt gtaaattggc ttctgttgac ttcttgagtt gcaataacga
    15061 ttgtgagaac tctttctgag aatctgtagg gagggataat ttttgatcaa tatctaaaat
    15121 tctttgcttg gtgttagtta tttttatttg tatcaattga agcagcgtgc gaatttccaa
    15181 atattcttga accatatcgt catattgttc ataaattgtt acaaattgct ggtaagcttc
    15241 gtggaaattg ctaatctctg gtttaaattt aattatttta tgacggaaaa gaaaatggat
    15301 aagcttgatt gcattgccat gcaagtcgcc cagtgaaatg gaacctaaat tatcttccaa
    15361 ttcattagga taattgtata tatcgatatt cttgttaatg agacgttttg gcattcccta
    15421 gtccatgaaa aaaac
//
LOCUS       DATNSN010000077        15418 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.077, whole genome shotgun sequence.
ACCESSION   DATNSN010000077 DATNSN010000000
VERSION     DATNSN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.077"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(165..240)
                     /locus_tag="VAH76_001859"
     tRNA            complement(165..240)
                     /locus_tag="VAH76_001859"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Asn,seq:gtt)
     gene            complement(300..1478)
                     /locus_tag="VAH76_001860"
     CDS             complement(300..1478)
                     /locus_tag="VAH76_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO1386472.1"
                     /translation="MDIALAKRVQKVKPSPTLAVAAKAAQMKAQGLDIIGLGTGEPDF
                     DTPQHIKLAAISAIEAGDTKYTAVDGIVELKEAVKNKFKRDNELDYQLNQILVSVGGK
                     QSCYNLCQAYLNPGDEVIIPAPYWVSYPDMVLLADGVPVIIETTPAQRYKINAQQLEQ
                     AITPKTRMIFLNSPSNPSGIAYTQNELKELGDVLKKHPQILIATDDMYEHIIWSQPFT
                     NILNACPELYDRTIVLNGVSKAYAMTGWRIGYAAGPAPLINAMKTIQSQSTSNPCSIA
                     QRAAVAALNGSNESIEEMVNAFHQRHDYVADRLQSIDGIEVIPADGTFYIFPSVQAII
                     EKRGYANDIEFSEKLLNEVGVALVPGSAFGTEGCIRISFATGIDTLKDALNRLQRFCS
                     "
     gene            1525..3516
                     /gene="uvrB"
                     /locus_tag="VAH76_001861"
     CDS             1525..3516
                     /gene="uvrB"
                     /locus_tag="VAH76_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEO1386473.1"
                     /translation="MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSG
                     KTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLH
                     LSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFD
                     DEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKVKAELQERLAELNA
                     QNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPDAL
                     LIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYI
                     SATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILV
                     TTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERMEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADTMTGSMQRALME
                     TERRREKQKAFNLKHGITPKGINKSVEDILEGAYIGKRKTMVAEQAPRYTHWSPQELA
                     KQINALEKQMYAHAQNMEFELAAKIRDEYLLLKEQLMKI"
     gene            complement(3511..3673)
                     /gene="ssrS"
                     /locus_tag="VAH76_001862"
     ncRNA           complement(3511..3673)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VAH76_001862"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3871..5484
                     /locus_tag="VAH76_001863"
     CDS             3871..5484
                     /locus_tag="VAH76_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEO1386474.1"
                     /translation="MNIKSNLAYSSVILKGHVNRYAFLGLLISVGSILIASCIVSYQL
                     TGSVSLGGFIQAQRTNPAIWILDLTPFMFVYWGQAFCYGLVNKAESLLVDKTKELLNI
                     SGNLELKLKYESNHDSLTNLPNNRLLNEKIRLAIEQLGLQGELAVIVIKINDLNYNFS
                     SFNANNIVKQFAEKLKSILIDPYMLKASLGINMVARLQSDEFAILMPRLKKDLDINEL
                     LIHLLTLLNNNLMVDGISINVTSTVGAAIYPAHGEEDDVLVNHAIIAVYQARKENKSY
                     AIYSPEMEEDLIQNRIVMNELQRSIENKDLKIYYQPIVELANGQIVGAESSARFEHPE
                     LGLLSAEKFMPLIEGTSLIHNLTTLMLKEVIKQLAAWHDAGHKIFVSVNLSVHHELPE
                     LIEKLLNDYEIAPEFLKLEFTERACLTEQMVTKEVFDRLSTMGIKLCIDDFCSENSSF
                     IHLTNFPIENIKIEKSFVLKIAKDAKKAKIVEAIVKLAQTLGWEPLADGIADQNALEK
                     LRDLGCLYGQGLYFSRAVNVAEFTSLLKK"
     gene            complement(5575..7146)
                     /locus_tag="VAH76_001864"
     CDS             complement(5575..7146)
                     /locus_tag="VAH76_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEO1386475.1"
                     /translation="MRRQWYIGFGVILLCLFALFAMLQDIVWFFAFLIPIVILWIYDV
                     LQTKHTILRNFPVLGHVRYFLEFLRPEIQQYFIATDESELPFNRETRSMIYQRAKNVR
                     DTIPFGTERDILSVGYTWALHSLSPKHSSEVEPRIDVGGPDCKKPYNASRLNISAMSF
                     GSLSSNAIMALNKGAKLGGFYHNTGEGGLSPYHLQGGDIVFQIGTAYFGCRDDKGNFD
                     ENEFIKEASRDEVKMIEIKLSQGAKPSHGGILPAAKLTEEIAKVRKVPMGKDVLSPIA
                     HTAFDNPVGLLHFVKRLRDLSGGKPVGFKLCLGRRHEFMAICKAMLKTNILPDFITID
                     GAEGGTGAAPVEYTNFIGTPLEAGLVFVHNALVGTGVRDKIRVICSGKVTNGFDLLTN
                     IALGADICNSARAMMMAIGCIQSKQCNANTCPTGVATQKKRLQYGLVVDEKKFLVANF
                     HNNTIKSFLEMVGALGLNNPSDLKPSHIMRRIGVDEVKSFDQIYTYVKLGQFLQGDIP
                     DDYRIHWEYADPDKF"
     gene            complement(7224..7538)
                     /locus_tag="VAH76_001865"
     CDS             complement(7224..7538)
                     /locus_tag="VAH76_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEO1386476.1"
                     /translation="MTNIKLCKIKLLNKIYEIKCPDGEEVSLILAAEKLNEKMQNNKN
                     KFKHLDNFHILLLAALDIGHELIVCKNEQEQQRLQVTQFISSLENKINKAVVGSETDS
                     NQ"
     gene            7694..8275
                     /locus_tag="VAH76_001866"
     CDS             7694..8275
                     /locus_tag="VAH76_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEO1386477.1"
                     /translation="MSLDNDSLHLPKYDDFVQSISVLALTMSGSELHGIMCGYLCAGA
                     DSQGEAYIRALLNNKKDEQSRNALLSMFSVFSISQQQMNNFDFEFEMLLPDDDESLVT
                     RAQAFSEWCEGFTQGLTIAGVGMEQFYEEESQDALQHLIEFAELDCESLEVGEEDERA
                     LMEVSEYTRMAVLRLHSDLVLHERELGDSGTTH"
     gene            8380..9690
                     /gene="pepP"
                     /locus_tag="VAH76_001867"
     CDS             8380..9690
                     /gene="pepP"
                     /locus_tag="VAH76_001867"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEO1386478.1"
                     /translation="MISQQEYKARRKKLAAQLPDDSVAVIPAAHEVLRSGDTHYRFRQ
                     DSDFYYLTGFNEPDALLVIIAGKDSQSILFNRPRNPAEEQWTGKRLGQDGALQELGVQ
                     AAYPLSSIAEELPKLFSGKSAIYYDLGRNLSLEKKIRHVLSSLKAQVRRGIKAPESLC
                     DLEPILSEMRLLKSDAELELMRQAARISVAAHKRAMQVCQKMEYEYQLEAELIYEFSR
                     HGCRSVAYDPIVGGGANACILHYTENNKPLRRGDLVLIDAGGEYGSYAADITRTFPVS
                     GTFSPEQKIIYELVLKAQKAGIAAIEPGLPWNHIQQTIIRILTEGLCDIGILKGDLEE
                     LIKNEAYKPFYMHNSGHWLGLDVHDVGRYKINNEWRPLEAGMVLTVEPGLYISAGMEG
                     VDKRWWDIGVRIEDDILVTRAGYEVLTGDLPVNVDDIEALMRGE"
     gene            9680..10882
                     /gene="ubiH"
                     /locus_tag="VAH76_001868"
                     /gene_synonym="visB"
     CDS             9680..10882
                     /gene="ubiH"
                     /locus_tag="VAH76_001868"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEO1386479.1"
                     /translation="MANKETDILIIGGGLTGASLSLALQGLGFSVTLVESRPYSDKVN
                     PDFDARSLALSPASQRILAMLGVWEILKEYTTPIHMIHVSDQHRFGVSRLHGEKNSPL
                     GYVVEMQYINRALSILLNKEHLLAPAALKSIDFNNSTATVAHGDDMISIQAQLIVAAD
                     GTESMTRHLCNLPVKIKDYAQDAIVANVGLIKPHGNRAFERFTPNGPLALLPMMNDKM
                     SLVWASKPEEAKRLVMLNDGDFLRELQTAFGYRLGRFGKVGKRYSYPLKQVLMPQQTK
                     WPVVFVGNAAHTLHPVAGQGFNLGLRDIAALAQCVSQKGLNPQMLEHYLKLRAHDQQM
                     ITWLTDGLIHLFTSRLPGMGVARNLGLLALDNIPALKNLLARYARGFGGITPDLVCEI
                     ALSTGEFQ"
     gene            10879..12042
                     /locus_tag="VAH76_001869"
     CDS             10879..12042
                     /locus_tag="VAH76_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEO1386480.1"
                     /translation="MSQHFDVLVIGGGIVGLTAALAMAQRHYTVAVIDAGTLMVNTDK
                     SDLRVYAINKASKMLLSELGVWQHLDDARVAPYNKMHVWDGVSGAYIDFDSRSIAEPN
                     LGAIIEESILKQALLRRISSESKINLFPQSVIDEVCHLERSIKISGQNTAWEGQLLMI
                     ADGANSPMRHKLKVDVTTWSYEQQALVATVNSEKSHQNTAFQVFHPDGPLAFLPLSNL
                     NQCSIVWSTHADNAMRLKLLDDNEFSRELTKAFGGQLGQIKVASNRHQFPLQMRHVKQ
                     YAGHRWLLLGDAAHTIHPLAGLGLNVGLADVKSWLTCLEMGKGELISKKVLGTYQRER
                     KHSVWQIILLMEGFKRLFGTSASPVTSLRSLGLRACNEFIPIKRLFMRQASGL"
     gene            complement(12371..13318)
                     /locus_tag="VAH76_001870"
     CDS             complement(12371..13318)
                     /locus_tag="VAH76_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HEO1386481.1"
                     /translation="MINLFRRLKNHGVLSINQRNTDFVLRYNPRKLFPLVDDKLKTKK
                     LALKAGIAVPALYDIIETEQQIKSIETRFASYNDFVVKPARGSGGDGILVFKDKVYGR
                     YRQINGKLTTVQELSYHLSCLLSGAYSLGGSSDYAIIEKRVVVDPVFAEVSYEGIPDI
                     RIITLLGYPAMAMVRLPTRLSGGKANLHQGAIGVGVDLATGKTLGGVFQNDTIDYHPD
                     TLNPIVGIEVPYWNQILEIASSCYELTGLGYLGVDIVLDKEHGPLMLELNARPGLNIQ
                     IANREGGLKRYRTIEARFKENGKESTLDKVNFCRKEFAR"
     gene            complement(13315..14838)
                     /locus_tag="VAH76_001871"
     CDS             complement(13315..14838)
                     /locus_tag="VAH76_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HEO1386482.1"
                     /translation="MKNNKRHVYGLILTLLLLGSGIFLYRHIILDVPLTDTETINSWM
                     VESNLRFTADRNTPIKASFNIPYLPPNFAILDEYFVSRNYGVTTNLNGSNRETVWSIR
                     RGNGPQSLYYRAIFRQTDSDESSLPKPSVTKSQPLNDSQKSAVETITNQVRSTSADIQ
                     TFAQSTIKELNKRDGNAKLLVGNEFNDDNIINATILILNQSKIPAITVQGIYLNQQKK
                     ADLKSLLAVFNGKNWIYINPKTGSAGLPKEFLIWQYGNGPLFNVVGGNRAQFSLTVSP
                     TPINALSVAKSRGLEDSQLLRFSLLQLPVNVQGIYKILLTVPIGAFIILILRNFIGIK
                     TFGTFMPVLIALAFRETHVAWGITLFVIIISFGLLARFYLDQLRLLLVPRLAAILTVV
                     ILLMIFISVLCQNLSLDTGMSVALFPMVILTMTIERMCITWDERGASEAIKSGIGSLA
                     AAVISYGAMSYEPLQYLIFAFPELLLVLLSLILWFGQYRGYRLVELKRFKSLASAMK"
     gene            complement(14828..15355)
                     /locus_tag="VAH76_001872"
     CDS             complement(14828..15355)
                     /locus_tag="VAH76_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEO1386483.1"
                     /translation="MRSKFVLFMLMSLLTGSLMANSEAQIYGYVEKATLIDQNLTLSA
                     KLDTGAKSASLHAVNITEIEKKGIPYLRFTVPTKTGDYSFEGEYVGKVKIKVRSSETN
                     PGLLRTTPIKRPVVLLNIKLGDKVRTIKVNLTNRKRFLYPLLLGRDAIIDFNGAVDPA
                     LTFTTKSKSPVNNEK"
BASE COUNT         4469 a         2865 c         3388 g         4696 t
ORIGIN      
        1 tttcaattta gatttgttat ttattaaagt atttccttta gtttagctta aaaagcaaaa
       61 gaaagaagtg caagtcgagt tttaaatttt taaaatcggt accaaaactg gtaccaagct
      121 tcaggagggg aaaacttgga attcgccaaa gcctagactg gaattggctc cccggacaag
      181 actcgaactt gtgacctaat gattaacagt catccgctct accgactgag ctaccgggga
      241 attgaggcgg aatcttaaaa cgattccgcg agagaatcaa gaggttttta atatattttt
      301 tagctgcaga aacgttgtaa tctgttcaag gcatctttca gggtgtcaat accggtggcg
      361 aaggatattc tgatgcagcc ttcggtgccg aaagcagagc ctggaaccag tgccacacct
      421 acttcattca gcaatttttc ggaaaattcg atgtcattgg cgtaacctcg tttttcgata
      481 atcgcctgca cactgggaaa aatgtagaaa gttccgtcgg cggggatgac ctctatgcca
      541 tcgatgcttt gcaatctatc agccacgtag tcgtgccttt gatggaaggc gttgaccatt
      601 tcttcaatgc tttcattgct gccattcaaa gctgctacag cagctctttg tgctatagag
      661 catggattgg atgtggattg ggattgaatg gttttcatcg cattgattaa tggcgcaggg
      721 cctgccgcat agcctatccg ccagcctgtc atggcatacg ctttggacac gccatttaat
      781 acgatggttc tgtcatataa ttcagggcag gcattgagga tgttcgtaaa gggttggctc
      841 cagataatat gttcatacat gtcatcagta gcaataagta tctgtggatg ttttttcaaa
      901 acgtcgccta attcctttaa ctcgttctgg gtgtaggcta ttccagaggg gttggaaggg
      961 ctgttcagga aaatcattcg cgtttttgga gtgatggctt gctccagttg ttgtgcattg
     1021 attttataac gctgcgcggg agtcgtttca atgatgacag gaacgccatc agccagcaaa
     1081 accatgtcag gataggatac ccaataagga gctgggatga tgacttcatc gccagggtta
     1141 aggtaagctt ggcagaggtt atagcagctt tgtttacccc ctacggagac caatatctga
     1201 ttgagttgat aatccagttc attgtctctt ttgaatttgt ttttaaccgc ttcctttagt
     1261 tcgactatcc catcaacggc ggtgtatttg gtatcacctg cttcaatagc agagatggcg
     1321 gctagcttta tatgctgcgg cgtgtcaaaa tcaggttcac cagttccaag gccaatgata
     1381 tcaagcccct gggctttcat ctgcgctgct tttgcagcta cggcaagggt aggtgaaggt
     1441 tttacttttt gtacacgctt tgccaatgcg atatccatct gttgctcctg tgaattatag
     1501 gggtactagc atataatata aaatatgaaa gacttattta aaatttactc aaattaccaa
     1561 cctgccggcg atcaacctac agccatagcc tctttgattg atggtcttga atcaggttta
     1621 gccaaacaaa cattattagg cgttaccggt tctggtaaaa catttactat agctcatgtt
     1681 attcaagcca tgaaaagacc cactttgatc atggcgccga acaaaacgct tgcggctcaa
     1741 ctttatggag agtttaaagc cttctttcct gataatgccg tagaatattt cgtttcctac
     1801 tacgattatt atcagcccga agcgtatgtg cctgcttcag atacttttat agaaaaggat
     1861 gcctccatta atgaacacat agagcagatg cgcttgtcgg caaccaaagc gttgattgaa
     1921 cgcaaagatg ccatcattgt agcgacggtt tctgccattt atggattggg cgacccggat
     1981 tcctatctgc gtatgttatt acatctctcc cgtggagaac aatccgatca gcgtaaaatt
     2041 ttaaagcgtc ttgcagaaat gcagtacaca cgaaccaatc tcagccttga gcgcggtcaa
     2101 ttccgggtac atggtgatgt cattgatatt ttcccggctg attccgagaa agaagcgata
     2161 agaattgagc tttttgacga tgaggttgat aatattgccc gctttgatcc tttaacagga
     2221 gaaattttac agcgactgcc ccgtgtcact atttttccta aaactcatta tgtcactccc
     2281 agagaacgta ttttagaaac agtagaaaaa gtaaaagcag aactacaaga aagactggct
     2341 gagttgaatg ctcaaaataa gttggtagaa gctcagcgtt tggaacaaag aacttgtttt
     2401 gatattgaaa tgatgttgga gctgggttat tgctctggca ttgaaaatta ctcgcgttat
     2461 ttatccaacc gcgaagccgg ggaagcaccg cctactcttt ttgattatct cccacccgat
     2521 gccttgttaa ttatcgacga atcgcatgtt actgttcctc aaataggtgg aatgtatcgc
     2581 ggagacaggg cgcgcaagga aaccttggtt aattatggat ttcgcctgcc ttctgccttg
     2641 gataataggc ctttgcgttt tgaagaattt gaagagcgtt cgccgcagac tatttatatt
     2701 tcagcaactc cagggcctta tgaacaggaa cattcggata atgtggctga gcaagtggta
     2761 agaccgaccg gattgattga tcctgaggtg gaaatcaggc cggtaaaaac tcaggtggat
     2821 gatcttatgt ctgaaatcag acaggtgatc gctcagggca gccgtattct tgtcacgaca
     2881 ttaaccaaac gcatggcaga ggatttgaca gagtatctga gtgagcacgg gatcaaggtg
     2941 cgctatttgc attccgacgt cgatacggtt gagcgcatgg aaattattcg tgatttgcgt
     3001 ttgggtgagt ttgatgttct ggtagggatt aatttattgc gtgaaggtct tgatatgcct
     3061 gaggttgctc ttgttgcgat tttggacgct gacaaggagg gttttttgcg atcggagcgt
     3121 tctttaattc aaacgattgg ccgtgcagca cgtaatgtga aggggcgagc tattttgtat
     3181 gccgatacca tgaccggttc gatgcaaaga gcattgatgg aaacagagag acgacgtgaa
     3241 aaacaaaagg cattcaattt gaaacatggc attactccca agggtattaa taaatcagtt
     3301 gaggatattt tggagggtgc ctatatcggt aaacgaaaaa caatggttgc tgaacaagct
     3361 ccccgatata cccattggtc tcctcaggaa ttggcaaaac agattaatgc actggaaaaa
     3421 caaatgtatg ctcatgctca aaatatggaa tttgaattgg cagctaaaat tcgggatgaa
     3481 tacctgctat taaaagagca gctcatgaag atataacccg atgggtaccg gattttgaac
     3541 ccttaggttc aagtgggcag cgaatgcatc ggttcaggct tcccactcta ttggtgggct
     3601 tgctcaacgc cgataacagg atgcttccct ggctgtgagt gttggttcta aaatcactcg
     3661 ccatcgggtt aattagatta taacattaac ctggcttcta catctataaa aactccatac
     3721 gatagagcat ttgttatttt ttctttaggc tatactaatc attaagtagt ccatttattc
     3781 agttttttgt agagcattga agtcgctgta gaagggatga ggtattaaaa attagcctgt
     3841 tgacaggcat aagggaatta ctgttactcc atgaatatta aatctaattt ggcatatagc
     3901 tctgttatcc tgaaaggtca tgtcaatcga tatgcctttt taggattgct tatttctgtt
     3961 ggcagtatct tgattgcaag ttgtatcgtt tcctatcaat tgactggatc tgtgagttta
     4021 ggcgggttta ttcaggctca aagaactaac ccggcaattt ggatcctgga tttaacacct
     4081 tttatgtttg tgtattgggg gcaagcgttt tgttatggat tggttaacaa agcggagagt
     4141 ttgctggttg ataaaaccaa agagctttta aatatcagtg gaaatctgga attaaagctc
     4201 aaatatgaaa gtaatcacga tagcttgacc aatttgccta acaaccgctt gttaaatgaa
     4261 aaaatacggc tcgccatcga acaattgggc ttacagggtg aattagctgt tattgtgata
     4321 aaaattaatg atcttaatta taatttcagc tcatttaatg ccaataatat cgtaaagcaa
     4381 tttgctgaaa aattaaaatc gattttgatt gatccctaca tgctaaaagc ttctcttgga
     4441 attaatatgg ttgcaagatt gcaaagtgat gaatttgcca tattgatgcc gagacttaaa
     4501 aaagatttgg atattaatga gctattaatc catcttctta ccttgctgaa taataacctg
     4561 atggtggatg gaattagtat caatgtgaca tctaccgttg gtgctgctat ctatcctgcc
     4621 catggagagg aagacgatgt tttagtaaat catgccatta ttgcagttta ccaggcacga
     4681 aaagaaaaca aatcctatgc aatttacagc cctgaaatgg aagaagacct tatacagaat
     4741 aggattgtga tgaatgaact gcagcgctcc attgaaaaca aagatttgaa aatttattac
     4801 cagccaatag ttgaactggc taatggccaa attgtaggtg ccgagtcatc agctcgcttt
     4861 gaacatcctg aattgggttt attaagtgca gaaaaattca tgcctttaat cgagggaacc
     4921 agtttaattc ataatttgac aactcttatg cttaaggaag tcataaagca attagccgcc
     4981 tggcatgatg ctggacataa aatttttgtc tctgtaaatc tgtcagtgca ccatgagttg
     5041 cctgaattaa tagagaaatt attaaatgat tatgagattg ccccggaatt cttgaaactt
     5101 gaatttaccg aaagggcttg tttaactgag cagatggtta ccaaagaagt gtttgaccga
     5161 ttatcaacca tgggcatcaa attatgcatt gatgattttt gcagtgagaa ctcttccttc
     5221 attcatttga ctaattttcc tatcgaaaac attaaaattg agaaatcatt cgtactgaaa
     5281 attgcaaaag acgcaaagaa agccaaaatt gtagaggcta tagtaaaact agcccaaaca
     5341 ttagggtggg aacccctggc agatggcatc gctgatcaaa atgcattgga aaaattaaga
     5401 gacttaggct gcttgtatgg ccagggatta tatttttctc gagcggtcaa tgtggctgaa
     5461 tttacatcat tgctaaagaa atgaatctgg ctttaaatca cttactattc gttttcacgc
     5521 tttgattcac atggaatacg aatcacaacg ctgacagcta tggccgcact gatattaaaa
     5581 tttgtctgga tcggcatact cccagtggat tctataatca tcaggaatat cgccttgtaa
     5641 aaattggcct agcttaacgt aagtatagat ttgatcaaaa gatttgactt catccacccc
     5701 gattcgtctc ataatatggg atggttttaa atcactggga ttgtttaatc ccaatgcgcc
     5761 aaccatttcc aaaaagcttt ttatcgtatt attatggaaa ttagcaacaa gaaatttttt
     5821 ttcatcaaca acaagaccat actgaagacg tttcttttga gtagcaactc ctgtcgggca
     5881 ggtattggca ttgcattgtt ttgattgtat gcatcctatt gccatcatca tagctcttgc
     5941 tgaattacag atatcagctc caagggcaat attagtgagc aaatcaaacc cgttggttac
     6001 ttttccactg caaattacgc gaattttatc acgaacaccg gtgccaacaa gggcattatg
     6061 aacaaaaacc agaccggctt ccaaaggagt tcctataaaa ttggtgtatt caaccggtgc
     6121 tgctcctgtc cctccttcgg caccatctat tgtaataaaa tcgggtaaaa tatttgtttt
     6181 cagcatggct ttgcaaatag ccataaattc atgtcggcgc ccaaggcaga gtttaaagcc
     6241 tacgggtttt ccaccggaaa gatcgcgtaa tcgttttaca aaatgaagta aaccaacggg
     6301 attatcaaaa gcggtatggg caattggcga taaaacatct ttgcccatgg gtacttttct
     6361 tacttttgcg atttcttcag ttaatttggc cgcaggcaaa attccaccat gagatggctt
     6421 cgcgccttgt gataatttaa tttctatcat ttttacttca tcacgggatg cttctttgat
     6481 aaattcattt tcgtcaaagt tgcctttgtc atcacggcat ccaaaataag ctgtgccaat
     6541 ttgaaaaaca atgtcaccgc cttgcaaatg gtaagggctt aaccctcctt cccctgtatt
     6601 gtgataaaat cctccaagtt ttgctccttt atttaatgcc attatggcat tagaggataa
     6661 agagccaaaa ctcatggctg atatgttcaa tcgggatgca ttataaggtt ttttgcaatc
     6721 aggcccgcct acatcgattc ttggttccac ttcacttgag tgtttcggcg atagagaatg
     6781 gagtgcccaa gtataaccaa cgcttagaat gtctcgctcc gttccgaaag gaatggtgtc
     6841 tctgacattt tttgcgcgtt ggtaaatcat ggagcgggtt tccctgttaa aaggaagctc
     6901 gctttcatcc gtagcgatga aatattgttg aatctcaggg cgaagaaatt ccaaaaaata
     6961 tctaacgtga cctagtactg gaaaattgcg taaaatggtg tgttttgttt gcaatacatc
     7021 ataaatccac aggattacaa taggaattaa aaaggcaaaa aaccagacaa tatcctgcag
     7081 catggcaaac agggcaaaga gacataaaag gataactcca aatccgatat accattgacg
     7141 tcgcatagct aattcctttt aattgtttgc tattaatatc aatagtatag gatggtttgc
     7201 cgaggaatac ttaatttatc tgcttattga ttgctatccg tttcagagcc aactactgct
     7261 ttattaattt tattttccaa agaagatata aattgggtca cctgcaatcg ctgttgctcc
     7321 tgttcatttt tacatacaat taattcatgg ccaatgtcca gagcagcaag taaaaggatg
     7381 tgaaaattat ccaggtgttt aaatttattt ttgttatttt gcattttttc attaagtttt
     7441 tctgctgcga gtattaaact cacttcttcg ccatcagggc atttgatttc atagatttta
     7501 tttaataatt ttattttaca gagtttaata ttggtcatta ttacgccttt gtatttgcac
     7561 ggtactaagc aaatagtagc aattttgcct taatgcagca aattatgaaa atcataggtg
     7621 ctgagaaagt gcagcaggtg aacactgatt ctaaatggag tatcattagc tatcttttta
     7681 aatagggtta aatatgtctt tagataatga ttcgctgcat ttacctaagt acgatgattt
     7741 tgtgcaatcc atttctgttt tagcattaac tatgtccgga agcgaattac acggaatcat
     7801 gtgcggttat ctgtgtgcag gggctgatag tcaaggcgag gcttacatac gcgcgttgct
     7861 aaataataaa aaagatgagc aaagtcgtaa tgcgttatta tccatgtttt ctgtcttttc
     7921 aattagccag caacaaatga ataattttga ttttgaattt gaaatgttat tgcccgatga
     7981 cgacgaatct ttagtcactc gtgctcaggc ttttagtgag tggtgtgaag gatttacgca
     8041 agggttaact attgcaggag ttgggatgga gcagttttat gaggaggaat ctcaggatgc
     8101 tcttcagcac cttatagaat ttgcagagct ggattgtgag tcgttggagg taggcgagga
     8161 agatgagaga gcgttgatgg aggtcagtga atacactcgt atggcagtac ttcgattgca
     8221 cagtgatttg gttttgcacg aaagagaact tggagattct ggaacaaccc attgatagca
     8281 agttgagaag gtatcaaagt tgtttgtatt tattggtttc tttgattttt taaggattgc
     8341 tcatttgttc tatttattaa agtgaaaagg gaaattgata tgataagcca acaagaatat
     8401 aaggcgagaa gaaaaaaatt agctgcgcaa ttgcctgatg acagtgttgc tgtaatacct
     8461 gcagcacatg aagtgctgcg cagtggagat actcattatc gatttcgaca ggacagtgat
     8521 ttttattatt taacaggatt taatgagcct gatgctttgc tggtcattat tgcgggaaag
     8581 gatagccaaa gtattttgtt taatcgccca agaaatcctg cagaagaaca atggacaggt
     8641 aaacgattag gtcaggatgg cgctttgcaa gagcttggag tgcaggcggc atacccatta
     8701 agcagtattg cagaagagct tcctaaacta ttcagcggga aatctgccat atactatgac
     8761 ctcggccgta atttatcatt ggaaaaaaaa atcaggcatg ttttatcttc actaaaagct
     8821 caggtgagaa gaggcattaa ggcacctgag tcgttatgcg atctcgaacc tattctaagc
     8881 gaaatgcgtt tgcttaaaag cgatgcggaa ttagagttaa tgagacaggc agcccgtatt
     8941 tcagtggctg ctcataaacg agcgatgcaa gtttgtcaga aaatggaata tgagtaccaa
     9001 ctggaggcag aattaatcta tgagtttagc cgtcatggct gtcgtagtgt agcttatgac
     9061 cctatcgtgg gtggtggcgc caatgcctgc attcttcatt atacagaaaa taacaaacct
     9121 ttgcgccgtg gcgatttggt gctcattgat gcagggggcg aatatggtag ttatgccgca
     9181 gacattacca gaactttccc ggtaagcggt acttttagcc ctgagcagaa aatcatttat
     9241 gaattggtat tgaaagcaca aaaagctggc atagcagcga ttgaaccggg ccttccctgg
     9301 aatcatattc agcagactat tatcagaatt cttactgagg gtctctgtga tataggaatt
     9361 ttaaagggcg atttggagga gctcattaaa aatgaagcct ataaaccatt ttatatgcat
     9421 aattcaggac attggttagg attggatgtg catgatgtcg ggcgatataa aatcaataat
     9481 gaatggcgtc ctttagaggc cggaatggta ttaacagtag aaccagggct gtatataagc
     9541 gcagggatgg aaggcgttga taaacgatgg tgggatatcg gtgtgcgtat agaagatgat
     9601 attctggtaa ccagggcggg atatgaggta ttgaccggtg acttgcctgt taatgtagat
     9661 gatattgagg ctttgatgcg tggcgaataa agagactgat atactgatta ttggcggtgg
     9721 tttgactggc gcaagcttat ccctggctct gcagggatta ggtttctctg tcacgttagt
     9781 agaatccagg ccatacagtg ataaggtaaa tcctgacttt gatgctcgct cattggcatt
     9841 gtcgcctgca agtcaacgta tattggcgat gctgggggtt tgggagattc tcaaagaata
     9901 tacaacacca attcatatga ttcatgtttc tgatcagcat cgctttgggg tttctcgttt
     9961 gcatggcgaa aaaaatagtc ctttgggcta tgtggtagaa atgcaataca tcaacagggc
    10021 tttgagtatt ctactcaata aagaacactt gttagctcca gcggctttaa aatccatcga
    10081 ttttaacaat tctactgcca ctgttgctca tggcgacgat atgatttcta tccaggctca
    10141 attaattgtc gcggccgatg ggacggaatc gatgactcgc cacctctgta atctgccagt
    10201 caaaatcaaa gattatgctc aagacgctat tgtcgccaat gtgggcctga taaaacctca
    10261 tggtaatcgt gcttttgaga gatttactcc taatggtcct cttgcattat tgcctatgat
    10321 gaatgacaaa atgtcattgg tatgggcatc aaagcctgaa gaagcaaaac ggttagtgat
    10381 gttgaatgat ggtgattttt tgcgggaact gcaaactgct tttgggtata ggttaggacg
    10441 atttggtaaa gtaggaaagc gatactctta tcctctgaaa caagttttaa tgccccagca
    10501 aaccaaatgg cctgttgttt ttgttggtaa tgccgctcac actttgcatc cagttgcggg
    10561 tcaggggttt aacttgggtt taagagatat tgcggctttg gcacaatgcg tcagccaaaa
    10621 gggattaaat ccacagatgt tggagcatta tttgaaatta cgagcacatg atcagcaaat
    10681 gattacctgg ctaacagacg gtttaataca tctatttacg agtcgtttac ccggtatggg
    10741 agtagcgcgt aaccttggat tgctggcact tgataatata ccggcattaa aaaatttatt
    10801 agcacgatat gcgcgtggtt ttggcggaat tactcctgat ttggtttgtg aaattgcatt
    10861 aagcactgga gaatttcaat gagccaacat tttgatgtac ttgtcattgg aggcgggatt
    10921 gttggcttga cagcagcttt agccatggcc caacgccatt atactgttgc tgttatcgat
    10981 gcggggacat tgatggtcaa tacagacaag agtgatttgc gtgtttatgc catcaataag
    11041 gcatcaaaaa tgttattaag tgaattggga gtgtggcagc acctcgatga tgctcgggtc
    11101 gctccttata ataaaatgca tgtatgggat ggtgtgagtg gcgcatatat tgactttgat
    11161 tctcgcagta ttgctgagcc aaatttaggg gcaattattg aagaatcaat cttaaagcaa
    11221 gctctgttgc gtcgaatctc atcagagtcc aaaataaatt tatttcctca aagtgtaata
    11281 gatgaggtgt gccatttaga gcggagcatc aagatctccg gccaaaatac tgcctgggaa
    11341 gggcagttat tgatgatagc tgacggagct aactctccta tgcgtcataa gcttaaagtg
    11401 gatgtaacca cctggtctta tgagcagcaa gcgttggtcg caaccgtaaa tagtgagaaa
    11461 tcacatcaga atactgcttt tcaagtgttt catcctgatg gccctttggc ttttttgcca
    11521 ttatctaatc tcaaccaatg ttctatcgtt tggtcaactc atgccgataa tgcgatgcga
    11581 ttgaagttac ttgatgataa tgaatttagc cgggaattga ctaaggcatt tggaggtcag
    11641 ctagggcaaa taaaggtagc aagcaatcgc catcaatttc ctttacaaat gagacatgtc
    11701 aagcaatatg cagggcatcg ctggttattg cttggtgatg cagcccacac tattcatcca
    11761 ttagccggtc ttgggttaaa tgttggcctg gctgatgtga aaagctggtt aacctgtctt
    11821 gaaatgggta aaggtgaatt gatatccaaa aaagtattag ggacatacca aagggaacgc
    11881 aagcattctg tttggcaaat tattttgctt atggagggat ttaaaagatt atttggaacc
    11941 tccgcatctc ctgttacctc attacgcagt ctcggattgc gggcatgcaa tgaatttatc
    12001 ccaattaaac gattatttat gcgccaggct agtggtttat aagtaatttt tcgccaaagt
    12061 aaggcttttt ttcaaaaaag atataatgct taatttttag ttaataattg ttatgtataa
    12121 tttttttgct aacctaaaaa actctccgag gtttttaaat cacttattct tatttctgaa
    12181 gttttttagg ttgcccttta tttgttattt aaaacaaact ggcttcatat aaataaatca
    12241 gaaaaaattc attatcctta ttaaaattac aatttcaggc aggaatcgag tgtagaaaca
    12301 atgagtgcca ggcctgggtt tgattaatcc ataaagaaag aacctgcgtt ttccataact
    12361 ttatttggcc ttagcgcgca aactctttgc ggcaaaaatt aactttgtcc agagtagatt
    12421 ctttgccatt ttccttaaaa cgggcttcaa ttgttctata gcgcttaagc ccaccttctc
    12481 ggttggctat ttgtatattt aatcctggtc tggcatttaa ttccagcatt aatggaccat
    12541 gctctttatc aagtacaata tcaactccaa gataacccaa accagttaac tcatagcaac
    12601 tggaggctat ttcaagaatc tgattccaat aaggtacttc gatgcctacg atgggattta
    12661 acgtgtcggg gtggtagtct atggtatcat tttgaaacac gccgccaaga gtttttcctg
    12721 ttgcaagatc aacacctacg cctattgctc cttgatgtaa gttggctttt ccgccggata
    12781 atcgggtggg aagtcttacc atggccattg ccggatagcc taataaggta ataatacgta
    12841 tatcgggtat gccttcgtaa ctgacctcag caaataccgg gtcaacgacc actcgttttt
    12901 caatgatggc ataatccgag gacccgccaa ggctataggc cccggataac aagcaagata
    12961 aatggtaact cagttcctgg acggtagtca gcttaccatt aatttgtcga tatcgaccgt
    13021 aaactttatc tttaaatacg agtattccat caccaccaga acctctggcc ggttttacca
    13081 cgaaatcatt atagctggca aatcgggttt ctattgattt gatttgttgc tcagtttcaa
    13141 tgatgtcgta taaggcagga actgcaattc cagcttttaa ggccagcttt ttggttttga
    13201 gcttgtcatc aaccaaagga aataactttc ttggattgta tcgtaataca aaatccgtat
    13261 tccgctggtt aatgcttaaa accccgtgat ttttcaggcg cctaaacagg ttaatcattt
    13321 catcgcactc gcaagtgatt taaagcgttt taattcgact aaacgatagc cacgatattg
    13381 accgaaccat aaaatcaagg acaaaagcac caaaagtaat tcgggaaagg caaagatcaa
    13441 atattgtaag ggctcatagc tcattgcccc atatgaaata actgctgcag caagactgcc
    13501 aattcctgat tttattgctt cggaagcccc tctttcatcc catgtgatgc acatgcgttc
    13561 gatagtcatg gttaagatga ccatagggaa taatgcgaca gacataccgg tgtcaagact
    13621 aagattttgg catagaacac tgataaagat cattaataga atcactacag tgagaatggc
    13681 tgcaagtctt gggactaaca gtagtcttaa ttgatccaga taaaatcggg ctaatagtcc
    13741 gaaagaaata ataatgacaa ataaagtaat gccccatgct acatgagtct ctctaaaggc
    13801 cagggcaatc aagacgggca taaacgtacc aaaagtttta atgcctataa aattacgcag
    13861 tattaaaatg atgaatgctc caataggaac agttagcaag attttataga tcccttgtac
    13921 attgacaggt agctggagta aggagaagcg caagagttgc gagtcttcta gccctcttga
    13981 tttggccaca cttaaggcat taatgggtgt tggcgataca gttaatgaaa attgagctct
    14041 attaccccca acaacattaa atagagggcc attcccatat tgccaaatca gaaactcttt
    14101 tggcaagccg gcactgccag ttttggggtt aatgtaaatc cagttttttc cattaaaaac
    14161 agcgagcagg gattttaaat cggccttttt ttgttggttt agataaatcc cctggactgt
    14221 aatggccgga atctttgatt gattgagaat caaaatggtg gcattgataa tattgtcatc
    14281 attgaattcg ttcccaacta aaagcttcgc attaccatct cgtttgttta actctttaat
    14341 agtgctttgg gcaaatgttt gaatgtctgc tgatgttgat ctcacttgat tggtaatcgt
    14401 ttcaacggcc gatttttgac tgtcattcag aggttgtgat ttagttaccg aaggtttagg
    14461 caaagaagac tcatcactgt ccgtttggcg gaagattgct ctataataaa gtgattgagg
    14521 accattaccg cgccttatag accatacggt ttctctattg gatccgttta aattggtggt
    14581 gacgccataa tttctggaca caaaatattc atcgagaatg gcaaaattgg ggggcaggta
    14641 agggatatta aagcttgcct ttatcggcgt atttctatca gcggtaaaac gtagattgga
    14701 ttcaaccatc caactgttga tcgtttccgt atcggtcaga gggacatcga gaatgatatg
    14761 tctatataaa aaaatacctg atcctaaaag caatagagtg aggatcaaac cataaacatg
    14821 acgtttgtta tttttcattg ttcactgggc tcttggattt agttgtgaaa gtaagggcag
    14881 gatcaacggc cccattaaaa tcaataatgg catctcttcc caacaataaa gggtaaagga
    14941 atcgtttcct gttggttaag ttcactttaa ttgttctgac tttatcacca agcttgatat
    15001 taagtaagac taccggtcgc ttaatgggag ttgttcttaa taatcccgga ttggtctcac
    15061 ttgaccttac tttaatcttt actttgccga catactctcc ttcaaatgag taatcaccgg
    15121 ttttggttgg aacggtaaaa cgtaaataag gaatgccttt tttttcaatt tctgtgatat
    15181 tcacggcatg tagtgatgct gatttagcgc cagtatcaag ctttgcggat aaagtgaggt
    15241 tttgatcgat taatgtggct ttctctacgt agccatagat ttgagcttca ctgtttgcca
    15301 taagagatcc tgtcagtagt gacattaaca taaagagaac aaattttgat ctcatttaat
    15361 cctcatattg ggttttattt tggtgcaata tcgtagcagg tttaaggcat aagtcaca
//
LOCUS       DATNSN010000078        15255 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.078, whole genome shotgun sequence.
ACCESSION   DATNSN010000078 DATNSN010000000
VERSION     DATNSN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.078"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(3..1487)
                     /gene="pgi"
                     /locus_tag="VAH76_001873"
     CDS             complement(3..1487)
                     /gene="pgi"
                     /locus_tag="VAH76_001873"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO1386484.1"
                     /translation="MKSHTELLSWNLLQKEADRVRLNSDSLTCVVPDSNNYESSKQIN
                     CIEYDYSRQRVNRTIIDLLIDLANEVKLQEKIDNLINGKKINISENRPALHTALRDLG
                     NKSIMIDGLDIMSAVINTREKIKVISNQIREKRWLGYSGLPITDIVNIGIGGSDLGPR
                     VCIHALSNYVSKEFNYHFISDVDPASFSDVIAKINPQTTLFIVSSKSFTTKETLLNAR
                     KAFALYEDTASIDQHFIAVTAHPERAYQMGIKTILPIWDWVGGRFSFCSAVNLITAIA
                     IGYEQFVELLAGAHDVDTHVQFTDFKNNIPVLMALIGIWNNNFLNIHNLLILTYSKKL
                     EYFVPYVQQLDMESNGKSIDVNGRMVDYATGPIVWGGLGNQAQHSYFQLLCQGTHRCV
                     GDFITLKTNDEHEINSMCHYKMKVLSEGIQTIENPYGYIPGNMPMNHLILSDCSPYTL
                     GALVALYEHKIFVQSVIWNINPFDQPGIESAKSAHREITLSSES"
     gene            complement(1541..2620)
                     /gene="rfbB"
                     /locus_tag="VAH76_001874"
     CDS             complement(1541..2620)
                     /gene="rfbB"
                     /locus_tag="VAH76_001874"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO1386485.1"
                     /translation="MICQMLSTILQKSKNLTMKNQMNNLLVTGAAGFIGSNFVKFMND
                     KYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILDRSLVLSLLREYEIDTLVH
                     FAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVY
                     GSLEREEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQH
                     KEKLIPKVVYACVNQLPITVYGNGSNIRDWLYVMDHCEAIDTIIQKGALGEVYNIGGN
                     NELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNSKIQKELGWVP
                     QGDFVHKLSNTVQHYLTRYEREINV"
     gene            complement(2580..3464)
                     /gene="rfbD"
                     /locus_tag="VAH76_001875"
     CDS             complement(2580..3464)
                     /gene="rfbD"
                     /locus_tag="VAH76_001875"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO1386486.1"
                     /translation="MKILITGANGQVGTEIIKRFSSSEHEVYACTRDILDCSKLERVH
                     DVLSEIKPDLIINAAAYTAVDKAEDEPDLAHIVNAEFVSHLVNYCSFKNIPLIHLSTD
                     YVFDGEKEGAYHETDIPHPVSTYGRTKWEGEQAILSQLKKYIILRVSWVFGEQGKNFV
                     KTILNLASSRKELNIVADQWGRPTSARDIARVLFEIVQKTRHSSFDYWGLYHYAGQGV
                     TNWHEFANVFLNLAKEKQLLLTLTDLNPIKSQEYPTRATRPKNSVLDTTKIETILGIK
                     SCSWKNDLPDVIDHFTKK"
     gene            complement(3465..4028)
                     /gene="rfbC"
                     /locus_tag="VAH76_001876"
     CDS             complement(3465..4028)
                     /gene="rfbC"
                     /locus_tag="VAH76_001876"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEO1386487.1"
                     /translation="MNIINTQIDELKIIEPKIYGDERGFFYESFQAKRYEELLGITDR
                     FVQDNFSRSQKGVLRGLHYQSQQTQGKLVSVLAGEVFDVAVDIRLGSPTFGQWVGVIL
                     SGENKRQFWIPKGFAHGFYVLSAMADFAYKCTDYYYPESEFSIHYLDPQLAIDWPLGE
                     QVQLSPKDAAAKPLSLIDAELLPRYQT"
     gene            complement(4118..5620)
                     /locus_tag="VAH76_001877"
     CDS             complement(4118..5620)
                     /locus_tag="VAH76_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO1386488.1"
                     /translation="MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVT
                     DGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNR
                     PIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
                     KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDT
                     WNMSPVALERALRESKAKNQMPKAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAES
                     LGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQRARFLSTQAREPMP
                     HYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEI
                     TQGYSTRWLSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPH
                     DEQFSVSDYLFDHGICLPSGSNLTPRDIDRVIHCLNDIFIKYKQNNFEVA"
     gene            complement(5631..6239)
                     /locus_tag="VAH76_001878"
     CDS             complement(5631..6239)
                     /locus_tag="VAH76_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO1386489.1"
                     /translation="MHTNLKIIGCGGHCKVVIDALSLTQHSLQVSLCDSNKSLLGQEV
                     CGVLIDSTMDSLADYIGYVHVSIGNNQARKNVIDLMNSNANLFTIIHPAAIIAKSARL
                     GAGSFIAAQAILGPECQVGEGCIINHSAVVDHEVIVGSCSHIAPNSTLGGRVKIGERV
                     LIGAGAVVLPGVTIDDGAIIGAGSVVVKDVKENAVVKGVPAV"
     gene            complement(6229..7362)
                     /gene="neuC"
                     /locus_tag="VAH76_001879"
     CDS             complement(6229..7362)
                     /gene="neuC"
                     /locus_tag="VAH76_001879"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO1386490.1"
                     /translation="MIRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDA
                     LYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAIGHELLGFTEVFESETPDVVLLL
                     GDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
                     IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDS
                     IKIQFQSVIQAALATNLQIICLEPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFI
                     DCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLR
                     EALNKPKIKYFNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY"
     gene            complement(7362..8384)
                     /gene="legI"
                     /locus_tag="VAH76_001880"
     CDS             complement(7362..8384)
                     /gene="legI"
                     /locus_tag="VAH76_001880"
                     /EC_number="2.5.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetyllegionaminate synthase"
                     /protein_id="HEO1386491.1"
                     /translation="MTCFIIAEAGVNHNGDLQLAKELVYAAKESGADAVKFQTFKADT
                     LVNKTVEKAEYQKNNAPESSTQYEMLKALELSEEDHYLLSELANSLGIEFMSTGFDEQ
                     SIDFLISLGVKRLKIPSGEITNVPYLQHCASKKLPLIISTGMCDLQDVRVAIDTVKPY
                     YGNSLSDYLVLLHCTSNYPAAYQDVNLKAMQTLADEFQLPVGYSDHTLGILVPTLAVG
                     MGACVIEKHFTMDKSLPGPDHLASMNPEEMKNLVQSIRDAETVLGSGEKKPSDNELPI
                     RALVRRSITLRRDLVKGAQISKEDLILLRPGTGIAPSEISNIVGSRLSMNLSAGTTLL
                     WEHIEA"
     gene            complement(8381..9079)
                     /gene="neuA"
                     /locus_tag="VAH76_001881"
     CDS             complement(8381..9079)
                     /gene="neuA"
                     /locus_tag="VAH76_001881"
                     /EC_number="2.7.7.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N,N'-diacetyllegionaminic acid synthase"
                     /protein_id="HEO1386492.1"
                     /translation="MRILAVIPARAGSKRLPGKNTRLLAGKPLIAHTIVAALQSSCCE
                     EIVVSTDSKQIADVAVQYGASVPWLRSEDLATDTSDVIHTVIDLLFKFQQMEVFFDSV
                     LLLQPTSPFRKPETIRHAVEIHQATGKSVVSVSPISLKPSWCRSIDSQGNLVKPELFQ
                     DLEIYCNENPIYKLNGSIYIATAKQIIENKSFYSEPTKPLLLNSISESIDIDTPIDWA
                     LTEKLMELNQEALV"
     gene            complement(9092..9733)
                     /gene="hisH"
                     /locus_tag="VAH76_001882"
     CDS             complement(9092..9733)
                     /gene="hisH"
                     /locus_tag="VAH76_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEO1386493.1"
                     /translation="MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLV
                     LPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDL
                     IAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
                     AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI"
     gene            complement(9730..10491)
                     /gene="wbuZ"
                     /locus_tag="VAH76_001883"
     CDS             complement(9730..10491)
                     /gene="wbuZ"
                     /locus_tag="VAH76_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl amidation-associated protein WbuZ"
                     /protein_id="HEO1386494.1"
                     /translation="MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGA
                     DELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAI
                     NTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
                     GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCES
                     KADAVAMADVLHYKRIGLPEIRKYALDNGIHVRVL"
     gene            complement(10491..11846)
                     /locus_tag="VAH76_001884"
     CDS             complement(10491..11846)
                     /locus_tag="VAH76_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEO1386495.1"
                     /translation="MSKIYAPKSIDLSSFQLPDEKLQTKYGLPHKVEFCKSCVISNQR
                     PNSAVEYEHKKESKKHTIHFDDEGICDACRVAERKKSTINWEERDRQLRELCDRFRSK
                     DGSYDCVVPGSGGKDSFYAAHILKYKYGMNPLTVTWAPHMYTPWGWRNFQSWIHAGFD
                     NHLFTPNGRVHRLLTRLAVENLFHPFQPFMIGQKAYAPKMALLHKIKLVVYGENEAEY
                     GNPIGDTESAKRDWKYFTADDKSKIFLGGTSVQELKSDFGLNDNDLDAYLPADPQQIE
                     EQQVEVHYLGYYLKWHPQSCYYYSVEHGGFEASPERTPGTYSKYNSIDDKIDDFHYYT
                     TLTKFGIGRATYDASQEIRSGDITREEGVALVKRFDQEFPERFAEEIFKYLSINLKEF
                     PIASQMFEQPIMDRAYFMALADTFRSPHLWKKDGEQWKLRHQVTNLEKTKAEYLDLET
                     V"
     gene            12033..12851
                     /locus_tag="VAH76_001885"
     CDS             12033..12851
                     /locus_tag="VAH76_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386496.1"
                     /translation="MSLKAMYNQIAENYATANRFGSISESHHVAIEQMRKFHLGLKPH
                     YKILDLGVGNGSFLQKLHHLMPMANFTGIDVSSEMLKRASEALPLTTIEGSAAEANKF
                     LPAHSQDLVLAHFINAYIPINVLFDEAKYLTRANGHFSLITTTYDSFPVAQQQLANFI
                     AQDTILSRVVGHYYKSIVKNTTVAANLDELLLAFKQHQFNILDHQRIEIPITLNNIDE
                     LALFGIEGTWFLNTLSIRMLPKYFLIQRLKRLFSKIFTFPYHDTHIIDVVLARK"
     gene            12890..14398
                     /locus_tag="VAH76_001886"
     CDS             12890..14398
                     /locus_tag="VAH76_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEO1386497.1"
                     /translation="MKLISQAVKNYLPELSLRQKQTNNIIFITFWSQFSVYALNTVLV
                     LFLTRPLIAQGLGYSQAKAYAFIGVTQATGYLMPILGGYMADNIIGVRRSILLGSIML
                     ACAYLLVMLSGYTISSLGDQLFIIAFAFIPATNSLLMGTASSMVSHIYSDDAIKAKSA
                     MTYYYMAINVGALLATMIAPVLLESRYGPLSVLTLTFIGKSIAALNFAKRYSIYNSVI
                     WGMDKSKFSNQGLLRLVAYIAAIYSLTLYAYTHVYIASTLIGAGCAIGILWFLIKTIM
                     LKGETRSKQMIAVLLIIEAIVFFIIYNQMNSTLVLFAENNSDLSFLWFKISPAQYQML
                     NPLLILLIGSQLPRFYRFFSRFTIPYQFAAGTILSGIALLVMAFAAQNAINGLVNGNY
                     IALTYILISIAELWVSAIGLSMIGLYCDARAIAFAMGVWYLASSMSNAISGRIAGWVA
                     IPENINSALESLPYYKNYYLIMGISALGLGILMYFVAYYLHKIMKRRGIELA"
     gene            14799..15080
                     /locus_tag="VAH76_001887"
     CDS             14799..15080
                     /locus_tag="VAH76_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO1386498.1"
                     /translation="MFWVYILQCSDKSYYTGQTDNLEKRLTQHQDKMIPGCYTSTRLP
                     IQLKFSQEFMSREEALSAERQIKGWSRRKKEALINGDWQALSDYSKRKN"
BASE COUNT         4565 a         3219 c         2609 g         4862 t
ORIGIN      
        1 ctttatgatt cgcttgacaa tgtgatttct ctgtgagcac tcttggcgct ttcaataccg
       61 ggctgatcga atgggttaat attccatatc acactttgta caaaaatctt atgttcatag
      121 agtgccacca gagcccctaa tgtataagga gaacaatctg acaaaatgag atgattcatt
      181 ggcatattgc caggaatata cccataagga ttttcaattg tttgaattcc ctcacttaaa
      241 actttcattt tataatgaca catggaatta atctcatgct catcattagt tttcaaagta
      301 ataaaatcgc caacacatcg atgcgtaccc tgacataata attgaaaata gctatgctgt
      361 gcctgatttc ctaatccacc ccaaacgata ggtccagtgg cgtaatctac cattcttcca
      421 ttcacatcaa tggatttccc attactctcc atatccagct gttgtacata aggaacaaag
      481 tactccagtt tttttgaata ggtaagaata agtaaattat gaatattcaa aaaattgtta
      541 ttccatatac cgattaaagc cattagcaca ggaatattat ttttaaaatc agtaaattgg
      601 acatgagtat cgacatcatg ggcaccagcc agcaattcaa caaattgttc ataaccaatt
      661 gcaatcgcag taattaaatt aactgcggaa caaaaagaaa atcgaccacc aacccaatcc
      721 caaatcggta aaatagtttt aatacccatt tgataagctc tttccggatg tgcggttaca
      781 gctataaaat gttgatcgat agaggccgta tcctcataca atgcaaaagc ttttctggca
      841 tttaatagag tttcttttgt agtaaatgat ttggaagaaa caataaataa ggtggtttgc
      901 ggattaattt ttgcaataac atcgctaaaa ctggccggat caacatctga gataaaatga
      961 taattaaatt ctttggaaac ataattagat aaggcatgga tgcatacacg aggacctaaa
     1021 tccgagccac caatacctat gtttacaata tcagttattg gtaaacctga atagcccagc
     1081 cacctctttt ccctgatctg attggatata actttaattt tttcacgagt atttataact
     1141 gcggacataa tatccagtcc atcaatcatt atcgatttat tacctaagtc cctaagtgca
     1201 gtatgtagtg caggcctgtt ttcgctgata ttgatttttt taccattgat taagttatca
     1261 attttttctt gtaatttgac ttcattagcc aaatcgataa gcaaatcaat gatggttctg
     1321 tttactctct gcctggaata gtcgtactca atacaattga tctgtttact ggattcataa
     1381 ttgttggaat ccggaacaac gcatgttaga gaatctgaat ttagacgcac tctatccgct
     1441 tctttttgaa gcaaattcca tgacaataat tctgtgtgag acttcatgct atttctaatc
     1501 atcaaaataa agtctatgtt acctgattat tttcaattta ttaaacatta atttcgcgct
     1561 cataccgtgt taagtaatgc tgaactgtat ttgataattt atgaacaaaa tcaccttgtg
     1621 gaacccagcc caattccttt tgaattttac tattgtctat ggcgtatcgt ttgtcatgac
     1681 cttttctatc ttcaacaaaa gtaatcaatg aatgatatgg tttttccata ggttttaaat
     1741 catccatcat ctgacaaatc atttttatca aacttaaatt atccagttcg ttattaccac
     1801 ctatattata aacttcccct aatgctcctt tttgaataat ggtatcaatt gcttcgcaat
     1861 gatccatgac atacaaccaa tcgcgaatat ttgatccgtt gccataaacg gtgatgggca
     1921 gctgattgac acaagcatat actaccttag gaatcaactt ctctttatgt tgattaggcc
     1981 cgtaattatt cgaacaattc gaagtggtaa caggtaatcc ataggtatga taataagctc
     2041 tgactatatg atcagaactt gccttggacg ctgaataagg tgagttgggt tgatatgaat
     2101 ttttttcagt gaacgccggc tcttctcttt ctaatgaacc atacacttca tcggtggata
     2161 catgatgaaa ccgacacttt gatttatccc attgcctttc attcaaccaa taaatccttg
     2221 ccgcctctaa cagagtaaaa gttccaatta cattggtttc aagaaatatt tgcggattat
     2281 caatggagtt atccacatgg gattcggctg caaaatggac aagcgtatct atttcatatt
     2341 cccttagcaa ggataagacc agacttctgt ccaatatgtc tccttgcaca aaaaggtgat
     2401 ttttacattc tgccatttca ctcaaattgg ctttatttcc tgcataggtt aatttatcca
     2461 aactgattat tttgatctcc gggtatttat cgttcataaa tttaacaaaa ttacttccta
     2521 taaagcccgc agctccagtt accaaaaggt tgttcatctg atttttcata gtgagattct
     2581 tacttttttg taaaatggtc gataacatct ggcaaatcat ttttccaact acagcttttt
     2641 atacccaata ttgtttctat ctttgttgta tccagtaccg aatttttagg ccttgttgcc
     2701 ctagtaggat attcttgtga cttaattgga tttaaatcgg ttaaagttaa gagaagctgt
     2761 ttctcttttg ccaaattaag aaatacgttt gcaaattcat gccaattggt aacgccctgc
     2821 cctgcataat ggtaaagccc ccaatagtca aaggaagaat gtctggtttt ctgtactatt
     2881 tcaaacagta ctcttgctat atcgcgagct gaagtaggtc ttccccattg atctgcaaca
     2941 atattcaatt cttttctgct ggaagccaaa tttaaaatcg tttttacaaa atttttcccc
     3001 tgttcaccaa atacccagct aactcgcaaa ataatatact tttttagttg ggataaaata
     3061 gcctgttctc cttcccattt cgtacgtcca taggtactta ctggatgcgg aatatcggtt
     3121 tcatgatagg ctccctcctt ttctccatca aatacataat cagttgaaag atggatcaag
     3181 gggatgtttt tgaagctaca ataattcacc aggtgagaga caaactcagc gttcacaata
     3241 tgagccaaat caggttcatc ctctgcctta tctacagccg tataggctgc tgcattaatg
     3301 ataagatccg gtttgatttc cgataacaca tcatgaaccc gttcgagttt ggaacaatct
     3361 agaatatctc tggtacaagc atatacttca tgctcagagg aagaaaatcg cttgataatc
     3421 tcggtaccaa cttgcccatt agcacctgta atcaatattt tcatttaggt ttgatatctc
     3481 ggtaaaagtt cagcatctat taaacttaaa ggcttggctg cagcgtcttt tggagacaat
     3541 tgaacttgct ctcctaatgg ccaatcaatt gctaattgcg gatcaagata atgaatggaa
     3601 aactcacttt caggataata atagtcagta catttataag cgaaatcagc catagcactc
     3661 aaaacataaa acccatgagc aaacccttta ggaatccaaa attgcctttt gttttctccg
     3721 gagagaatca ctccaaccca ttgcccaaaa gtaggggagc ctaacctaat atccacggca
     3781 acatcaaaca cttcaccagc taaaactgaa accagttttc cctgggtttg ttgactttga
     3841 taatgcaaac ctcttagtac ccctttttgt gatcttgaaa aattatcttg cacaaaacga
     3901 tcagttattc ctaataactc ttcatatcgt ttagcttgaa aagattcata aaaaaaaccc
     3961 ctttcatcac cataaatctt tggttcgata attttaagtt catcgatctg agtatttatt
     4021 atattcatat cgttactaaa gctcttaaaa gagaattata attccttaat atttaaatct
     4081 tttactacct gtatccagtt ttcactagaa caaataatta agctacctca aaattgtttt
     4141 gtttatattt aataaaaata tcattcaaac aatgaatgac tctatcaatg tctctggggg
     4201 ttaaatttga cccggaagga agacaaatac catgatcaaa caagtaatct gaaacgctga
     4261 attgttcatc atgaggatag tatttagagc ctgcaaataa aggctgtcta tgcataggct
     4321 tccatgttct tcttgcttca atgttaaatg gctttaattg ctcaatgact tcttccggtt
     4381 ttatcttgtt tggcgttggt ttaataacca atgtcgataa ccaacgagta ctgtatcctt
     4441 gggttatctc tggcatcatg tcaataaatg ggtaagattc caaggcttgc ttatatcgtt
     4501 caaaaatagc tcgtcttgcc ttaacccgtt tttccaatac tttcagctga cctctgccta
     4561 taccagcaag cacattactc attctgtaat tatacccaac cacagtatgt tcataatggg
     4621 gcattggttc gcgagcctga gttgataaaa atcttgctct ttgtatgaga ctctcatctt
     4681 cagacaccaa catgccgcca ccagacgtgg taatgatttt gttgccatta aatgaaaaca
     4741 cgcctaactt gccgaaagtc ccgctgtact tgttattata ggtggctccc aaagactccg
     4801 ccgcatcttc tataattggc acattgtatt cattgcataa ttgacataaa gcgtcataat
     4861 tagcactttg tccatataaa ttaacaataa tcacagcttt tggcatttga ttttttgctt
     4921 tactttctct taatgcccgc tcaagagcaa ctggagacat attccaggtg tccaaatcag
     4981 aatcaataaa taccggagtc gcaccttggt ataagatagg attcactgtg gctacaaaag
     5041 taaagctgga tgcgaacaca atatctcctg gtttcacatc gagtaatacg agtgccagat
     5101 gaattgcagc tgttcctgag cttaaagcca cagctgattt tatattaatg tattccgcca
     5161 cttctttttc aaaagaatca acattggggc ctaaaggggc tacccagttg gtggcaaaag
     5221 cttcctgtac gtattgctgc tcatgctcac cgatatgagg cgaggaaagc agaatattgg
     5281 aagagagttc tctccggtgg atcaagcgta tggggcgatt actctcatcg ataacgggaa
     5341 tatgatctaa ttcatattcc tgcatcaaat gataagcatc ctgaatcgta gcagagacag
     5401 gtaaagcttt tgatgaatgc tctgataatt cctctaaagt agaatctaat gtaaatcctt
     5461 ttaatagcaa tcttcggatg tcaccatctg taactgtcct tattaaatgt tcatcactat
     5521 ctaccagaaa taacacacct tgagctgtag catccagctt gctcaaagca gttttcagat
     5581 tttcttgttt ttgtatatat aactctttga tagtaatcat tttattcctt ttaaactgcg
     5641 ggtacgcctt tcacaacagc attttctttt acatccttca caacgactga gccagctcca
     5701 attattgctc catcatctat agtcacacca ggcaaaacaa ctgctccagc tccaatcagc
     5761 actctctcac caatcttgac tcgcccacct aaggtgctat taggcgcaat atgtgaacac
     5821 gagccaacta tcacttcatg atccacaacg gcactgtgat tgatgataca tccctcaccc
     5881 acctgacatt cagggccgag tattgcctgt gctgcaataa aggagcctgc accgagcctc
     5941 gctgatttcg caataatggc agccggatga ataattgtaa acaagtttgc attggaattc
     6001 atcaaatcta taacattctt tctggcctga ttatttccaa tgctaacgtg cacataacca
     6061 atatagtcag ctaaactgtc catagttgaa tcaatcaata ctccacacac ctcctgaccc
     6121 agcaaactct tattgctatc acaaagtgaa acttgtaaag agtgctgagt cagtgataaa
     6181 gcatcaatca ccaccttaca gtgtccacca catcctatta ttttcaagtt agtatgcatt
     6241 gcatttattc aatatttgtg agtgcaaagg gatagttttt aacaattgat aacacctttc
     6301 actcgttttt ccatccccgt aacaattaaa gtattttatc ttgggtttat ttagcgcttc
     6361 tcttagacca gtcaaaatag catcataagt aacatcaaca tcaatgacat tgtcgcttcg
     6421 ttctcttaaa ttttgcctgc ttccaacatt aactacgttc aggttaaatg aggctgcctc
     6481 tatgatgcca ctactggaat ttcccagcat cacatcagaa tttgcaagac aatcaataaa
     6541 ttccggacga tgtaggtgct tgataattct aacatcagga tgatcaatat attcctgaat
     6601 cacttctcga attaaatgac cacccgtatc tgaattcggc tcaaggcaaa taatctgcaa
     6661 atttgtagcg agtgctgcct gaatcacgct ttggaattga attttaatcg agtcaacttc
     6721 ttgaacaacc gggtgataga ttaataaaca aatttttttg tcaggatgaa atccataacg
     6781 ttgattaaac acatcacgtg tagacgtttt atactgcatg atttcatcca agcctggagc
     6841 accaacctga aaaatggttt cttccttctc acccattcta atcaatcgtt gtttggatgc
     6901 ctctgttgcg acaaaatgat aatgagataa tttggaaatc gcatgcctta ccatttcatc
     6961 aacggttcca gagcgctctc ctccgtgcag atgtacaacc gggatattta aatgtatcgc
     7021 tgctatggcc gcagcaagca tttctcctcg atctcccagc aataaaacga catcaggagt
     7081 ttcactttcg aatacctcgg tgaatcccaa aagttcatgg ccgatagctt ttgccataga
     7141 actatgctga gcattggcaa gatcaacagg aataatgccg catattgaga actgatctgc
     7201 tttaatttcg ttaactgtat ttccatacaa agcatcaaga tgcataccag tgacacaaat
     7261 cgataagtca atgtcttctg actggtgtaa tctttttagt acttctctca tcagtccata
     7321 atcggcacga gtacctgtaa cataaattat ttttctgatc attacgcttc aatatgttcc
     7381 cataacaaag tagtacccgc agacaaattc atacttaatc gagagcctac aatatttgat
     7441 atttcagagg gcgctatccc agtaccaggt cttagcaaga tcaaatcttc cttactgatt
     7501 tgtgctcctt tcaccaaatc acgtcttaaa gtaatacttc ttctaaccag agctcttatc
     7561 ggcagctcat tatccgacgg ttttttttca ccacttccca aaacagtttc agcgtctctg
     7621 atggactgca ctaaattctt catttcctct ggattcatag aagcaagatg atctggacca
     7681 ggaagagatt tatccattgt aaagtgcttt tcgataacgc acgcccccat tcctactgct
     7741 aatgttggca ccaaaatacc taaagtatga tcagaataac ctactggcaa ttgaaattcg
     7801 tcagctaatg tctgcatggc tttcaaattc acgtcctgat aagctgccgg ataattagag
     7861 gtacaatgca acaaaaccag ataatcggat aaagagtttc catagtaggg ttttactgta
     7921 tctatggcta ctcttacgtc ttgtagatcg cacatccctg ttgagatgat taatggtaac
     7981 ttcttactgg cacaatgttg taaataaggt acattagtaa tttcgccaga agggattttt
     8041 aatcgcttta cccctaaaga aataagaaaa tcaatgcttt gttcatcaaa accagttgac
     8101 ataaattcaa tgccgagtga attggctaat tcacttagta aataatggtc ctcttctgaa
     8161 agttccagtg ccttgagcat ctcgtactgg gtagaggatt ccggggcatt atttttttgg
     8221 tattccgctt tttctactgt tttattaacc aaggtgtccg ctttgaaagt ctgaaacttt
     8281 actgcatcag ctcctgactc tttggctgca taaactaatt ctttagccag ttgaagatca
     8341 ccattatggt ttactcctgc ttcagcaata ataaaacaag tcatactaga gcctcttggt
     8401 ttaattccat taatttttct gttagagccc aatcgattgg cgtatcgatg tcgatggatt
     8461 cactaatact atttaacaat aaaggtttgg ttggttcgct ataaaaactc ttattttcga
     8521 taatttgttt agctgttgca atataaattg acccatttaa tttataaatt ggattctcgt
     8581 tgcaataaat ttccagatcc tgaaaaagct ctggcttaac caaattgcct tggctatcaa
     8641 tgcttctaca ccaagaaggc tttaaagaga tgggactaac tgaaacgaca ctttttcccg
     8701 ttgcttgatg tatttcaaca gcatgtctta tggtttctgg cttcctgaat ggagaagttg
     8761 gttgtaaaag caatacactg tcaaaaaaaa cctccatttg ctgaaactta aacaggaggt
     8821 caataacagt atgaatcaca tccgaagtat ccgtggctaa atcttccgat cttagccagg
     8881 gtactgaagc cccatattga acggcgacgt ctgctatttg tttactatcg gtcgaaacaa
     8941 cgatttcttc acaacaagac gactgcaagg cagcaacaat agtatgtgca attaatggct
     9001 ttccggcaag taatctggta tttttaccgg gcagcctctt tgagccagcc cttgccggga
     9061 ttactgccaa tattctcatt tttaattcac cctaaatctg caaaaactgt tgaatgatgc
     9121 ttaaacctac ttccccgctt ttttcaggat gaaattggca tccgtacata ttttcatctt
     9181 ttattactgc gctaatagcc tgaccatcat ataaagtatc agccagacgc tttttaggat
     9241 tactaggcat ggccataaaa gaatgaacaa aatatactga agaatttaat ggtatatttt
     9301 tcaggatagt atgacaccaa tcctcgcctt ctgaggtaga aaccagttca ttccagccta
     9361 tatgaggtat tttatgcaat tgaccatcaa ctccatgtga aggaacgcta acaacctcgc
     9421 cagctattag atccaggcct tcatgttgtc cgaattcagt acttcttgag agcatcatct
     9481 gcatgccaag acatattccc agaaaaggtt ttccactccg cgcaaattgc tttatgggtt
     9541 ctataaaatt taatgtagtt aatcctttca tcccatcttc aaaagcgcct actcctggca
     9601 aaaccaaccg atcggcactc ataatttcct cgggtgtgct cactaaattg acagaagcat
     9661 caaaatattg aaaagctcga gccacactta ataaattgcc taccccataa tctacaattg
     9721 aaactgaact cataagactc tcacatgaat gccattatct aaagcatact ttctgatttc
     9781 aggtaaacct attcttttgt aatgcaaaac atccgccata gcgactgcat cagccttaga
     9841 ctcacaaacc acttcagtta aatcttccag cttacccata cccccacttg caatcaccgg
     9901 gatagaaacc atggaagaca cctgtttcac taattccaga tcaaatccct tacgcgtacc
     9961 ttcctgatca atagaggtca gaagaatttc acctgcacca aattcttcgc cattcctagc
    10021 ccaatcaaca acatccattc cggtatgttc acgtccattg tctgtcatga cctcccatct
    10081 tccattcaca gtgcgttttg cttcgataga taaaacaaca cattgagacc caaatcttct
    10141 ggcaatatca gatattagag tagggttttt agttgccgct gtattaatag ctaccttatc
    10201 ggcaccgcaa cgcaataatt gcttggcatc atcaacactg cgaattccgc cacctacagt
    10261 aattgggata aatacatttt cagcagtagt ctttacaatt tctgacaaat tattacgccc
    10321 atacaaagat gcaactgtat ccatataaat caattcatct gcaccttgtg cataatatgc
    10381 catagcatat tcattgggat ttcctactac tcttaatccc tccagatgca ctcctttgat
    10441 taggttaggt cctttaatat ctagtcgtgc aattagacgg acattactca tcaaacagtc
    10501 tccaaatcca gatattcagc tttagttttt tcaagatttg ttacttgatg tctaagtttc
    10561 cactgttctc cgtctttttt ccacaagtga ggggatctga aggtatccgc caaagccata
    10621 aaataggctc tgtccataat cggttgctcg aacatttgcg aagcaatggg aaactctttg
    10681 aggttaatac tcaagtattt aaaaatttct tcagcaaaac gctctggaaa ctcttgatca
    10741 aaacgcttca ccagagcaac accttcttca cgagtaatat cccccgaacg tatttcctgg
    10801 gaggcatcgt atgttgctct tccaataccg aatttggtta gcgtggtgta gtaatggaaa
    10861 tcatcaattt tgtcatcaat actattgtat ttactgtagg ttcctggagt tcgttcagga
    10921 gaagcttcaa aaccgccatg ttccacagaa taataataac aactttgagg atgccacttc
    10981 agataataac ccaagtaatg aacctctacc tgctgctctt cgatttgctg tggatcagca
    11041 ggtaagtaag catccaaatc attgtcattt aaaccgaaat cgctttttaa ctcttgaaca
    11101 gatgtaccac ctaaaaagat tttgctttta tcgtccgcgg taaagtattt ccagtctctt
    11161 tttgcagact ccgtatcacc tattggatta ccatattccg cttcattttc tccataaaca
    11221 accaatttaa ttttatgcaa caaagccatt ttaggagcat aagctttttg accaatcata
    11281 aagggttgaa atggatggaa tagattctca actgcgagtc gtgtcaatag cctgtgaact
    11341 ctgccatttg gagtaaacag atgattatca aatccggcat gtatccagga ttgaaaattc
    11401 ctccaacccc atggcgtata catatgggga gcccaagtca cggtcaaggg attcattccg
    11461 tatttatatt ttaaaatgtg ggcagcataa aaactatcct ttcctccgga accaggaaca
    11521 acgcaatcat aagaaccatc cttgcttcta aatcgatcac aaagctctct caattgcctg
    11581 tctcgctctt cccaattaat agtagatttt ttgcgttcgg caactcgaca ggcatcacaa
    11641 atgccttcgt catcaaaatg gatggtatgt ttttttgatt ctttcttgtg ctcatactct
    11701 acagcagaat taggcctttg atttgaaata acacagcttt tgcaaaattc caccttatgt
    11761 ggtagaccat actttgtttg tagcttttca tctgggagct gaaaacttga taaatcaatg
    11821 gactttggtg cgtaaatttt gctcatatca attcctatcc tggcattaga catttttaaa
    11881 aaaccgcagt atacaataaa aatttcaggt attaaacaaa tcctgattga agaaccaaaa
    11941 tatataattt aataaatttg tgttttcaat agtcgccaat tacccccttt tagggatata
    12001 ctggatgaat cgagttaaag taggaatttt gcatgtcctt gaaggccatg tataaccaaa
    12061 tcgcagaaaa ttacgcgaca gctaatcgtt ttggttcaat tagtgaaagc catcacgttg
    12121 ccattgagca gatgaggaaa tttcatttgg ggttgaaacc ccattataaa atcctcgatc
    12181 tgggcgttgg caatggctct tttttgcaaa aattgcatca cttaatgcca atggccaatt
    12241 tcacaggaat tgatgtttca tcagaaatgt tgaaacgagc gagcgaagcc cttcctttaa
    12301 caaccattga aggcagtgct gcagaagcca acaagttttt gcccgcacat agccaggact
    12361 tggttctggc tcattttatt aatgcatata tccctattaa tgttctcttt gatgaagcca
    12421 aatacttgac gcgtgccaac ggtcacttct ctttaatcac aacgacctat gattcttttc
    12481 ctgtagcgca acaacaactg gccaacttca ttgctcagga tactatttta agtcgagttg
    12541 ttggtcacta ctataaatcc atagtcaaaa atacaactgt tgctgcaaat ctcgatgaac
    12601 tcttgttggc atttaaacag catcaattca atattttgga tcaccaacgt attgaaatac
    12661 ccattaccct taataacatt gatgaattgg cactgtttgg aatagaaggc acctggtttc
    12721 ttaacactct atcgatacgc atgctgccaa aatatttttt aattcagcgt cttaaacggc
    12781 tttttagcaa gatttttact tttccctacc atgacactca tattattgat gtagttctcg
    12841 ccaggaagtg ataatcttga accgtaggtt agaaaaaacg gaatgaggtg tgaaattgat
    12901 ttctcaagca gttaaaaatt atcttccaga actaagtctt cgccaaaaac aaaccaataa
    12961 cattattttt attacttttt ggagccagtt ttctgtctat gctctgaata cagttctggt
    13021 tttattttta actcggcctt tgatagctca gggattaggc tacagccaag ccaaagccta
    13081 tgctttcatt ggtgtaactc aagctacagg ttacctgatg cctattttag gcgggtatat
    13141 ggctgataat attattggtg taaggcgatc cattcttttg ggcagcatca tgctggcttg
    13201 tgcttattta ctagttatgt tgagtgggta caccattagt tctcttgggg atcaattatt
    13261 tatcattgct tttgccttta tccctgcaac caactcacta ttaatgggga ccgcatccag
    13321 tatggtttct catatttatt ccgatgatgc cattaaagcc aaatcagcta tgacttatta
    13381 ttatatggcc attaatgttg gggcgctgct ggcaaccatg atcgcgccag ttctcctgga
    13441 aagtcgttat ggtccactat cagtgctcac cttaaccttt attggcaaat cgattgctgc
    13501 cttgaacttt gcgaaacgtt actccatata taatagcgtc atctggggga tggataaatc
    13561 caaattttct aaccaaggtc tattgcgcct tgttgcttat atagcggcta tctattcact
    13621 tactttatat gcttatactc atgtttatat cgccagcaca ctgataggcg caggatgtgc
    13681 tataggaatt ctctggtttt taattaaaac cattatgctt aaaggcgaaa ctcgcagtaa
    13741 acaaatgatc gctgtattac tgataataga agccatcgta ttttttatca tatacaatca
    13801 aatgaacagt accttggtat tatttgcaga aaacaattct gatctaagtt ttctttggtt
    13861 taaaatttca cccgctcaat accaaatgct aaatcctttg ctcattttac tgattggaag
    13921 tcaacttcct cgtttctaca gatttttttc gcgctttacg atcccctatc aatttgccgc
    13981 aggcacaatt ctctctggta tcgccctgct ggttatggct tttgcagccc aaaatgcaat
    14041 caatggatta gtgaatggta actacattgc tttgacctac attttaattt caattgccga
    14101 attatgggtt agtgccattg gtcttagcat gattggactg tattgtgatg cacgtgcgat
    14161 tgcctttgcg atgggcgtat ggtacctggc cagttccatg tccaatgcca tttcaggacg
    14221 aattgcaggt tgggtcgcca taccagagaa tattaattct gctcttgaaa gtttacccta
    14281 ttataaaaat tattatttga ttatgggcat tagtgctttg gggcttggta ttctgatgta
    14341 ttttgtagct tattatttac acaagattat gaaacgacga ggtattgagt tagcataacg
    14401 taactgaaca ttgtaatatt cgaatatgct tcgagacggt gcttcgcacc tcctcagcat
    14461 gaacggccag atgggaaaat ccaattttgt aatcagcctt ggagttttat gcaaaccatc
    14521 ttccgattat ccataggttt ttcttgttaa ttggataaat ccgttattat attaaaccgt
    14581 tcatgctgag gaagtgcgaa gcaccatctt gaagcaaaaa cggcccgatg ggaaaatcca
    14641 attttgtaat cagccttcgg attttaagca taccattttc caattatcca tagatcttaa
    14701 ttggataaat tagttattat cttaatccgt tcatgctgag ggggtgcaaa gcaccgtctc
    14761 gaagcatgct taaaaacata gtataaggga ttgactaaat gttttgggtt tatattcttc
    14821 aatgcagtga taagagttat tatacagggc aaacggataa tttggaaaaa cgccttactc
    14881 aacatcaaga taaaatgatt ccgggttgtt atacaagcac aagattacca attcaattaa
    14941 aattcagcca ggaatttatg tctcgtgaag aagccttgag cgctgagaga caaatcaaag
    15001 gatggagtcg gagaaagaaa gaggctttaa taaatggaga ctggcaagcg ttatcagact
    15061 attctaaacg taaaaattaa gagtgggctg tgtttcattc gaacatgctt cgagacggtg
    15121 ctctgcacct cctcagcatg aacggtttaa tataataact gatttatcca attaacaaga
    15181 aaaatttatg gataatcgga agatggtttt cataaagccc gaaggctgat tacaaaaagt
    15241 ggattttccc atcgg
//
LOCUS       DATNSN010000079        15246 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.079, whole genome shotgun sequence.
ACCESSION   DATNSN010000079 DATNSN010000000
VERSION     DATNSN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15246
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.079"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..222)
                     /gene="amrB"
                     /locus_tag="VAH76_001888"
     CDS             complement(<1..222)
                     /gene="amrB"
                     /locus_tag="VAH76_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEO1386499.1"
                     /translation="MSVRQPAVAGYFYPDDPLILKQTVLNFLDQAPIHKPAPKAILVP
                     HAGYVYSGSVAASAYASLRDKKNSINKIII"
     gene            294..1370
                     /gene="amrS"
                     /locus_tag="VAH76_001889"
     CDS             294..1370
                     /gene="amrS"
                     /locus_tag="VAH76_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEO1386500.1"
                     /translation="MFDTIPTKYWHKLEDGRIQCDVCPRNCKLREGQRGLCFVRANLD
                     NQIKLTTYGRSSGYCIDPIEKKPLNHFLPGSPVLSFGTAGCNLACKFCQNWDISKSRD
                     IDTLADTAMPEDIARAAQNMNCLSVAYTYNDPVIFMEYAIDVAKECHKLGIHSVAVSA
                     GYINPEPCKELYSFMDAANIDLKSFSEDFYYKITGSHLQPVLDTLIYLKKETSVWFEI
                     TNLVIPEENDSDAEFERMSEWIMTNLGPDVPLHFTAFHPDWKMMDKAPTPFSTLQRAR
                     EIAIKTGLHYVYTGNVHDTTGSSTYCPHCKLPVIVRDWYRILDYRVDDTGHCLNCHTQ
                     IAGVFKGAVGHWGPRRVMVKLGCS"
     gene            1429..1848
                     /locus_tag="VAH76_001890"
     CDS             1429..1848
                     /locus_tag="VAH76_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1171 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386501.1"
                     /translation="MRFQDSDFEERYNTMWNKIAVSADAQIRQLFGAKGFFSEQQPNY
                     YQLLVNYAQAAKNIVDNLNRQSPMFDDKEYVEGYMIATLQSVYKDFSQYKPRIAGRYG
                     EHSSCVELINKTLDWVQSFDLKLENLSESDNEMKITF"
     gene            1998..3482
                     /gene="lidL"
                     /locus_tag="VAH76_001891"
     CDS             1998..3482
                     /gene="lidL"
                     /locus_tag="VAH76_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LidL"
                     /protein_id="HEO1386502.1"
                     /translation="MQGGMMKQLVVAGFFLLLLTSPLSALDVDTYRSSQKQFNTEDKA
                     EQGDAQAQFELGLQYEKGDGVKKDLKKAIYWYQKAADQGQAEAQNNLGVLYLKGEGVP
                     QNSQQAMYWFKKASEQGLAIGQNNIGILYENGLGVKKDLGQAFIWYQKSAEGGNSDGQ
                     YNLAVMYMYGNGIPKDIKKAIHWYIKAAEQGDLDAQNNLGVLYERGEEVPRDLKAAIS
                     WYTRAANEGSLIAQTNLGVLYMTGDPSIQDGKKAIYWYEKAAAQGGEKAQNNLGYIYE
                     QGIGTEKDMKKAIYWYEKAAENGFTLAQNNLGVLYSNDGELQDYKKAYLWFKKAADQG
                     FAEAQNNLGLMYMKGNGLSVNYHEAVLWYKRAAEQGLPLAQHNLAIMYMKGLGIKKDN
                     KLAIKWYQKAAEKGLDLAQNNLAVMYIRGEGVKRDFKKAMYWYQKAAEQGLDLAQINL
                     GIMYLDGMGVNKDFAKAKYWIGKAKDSGSQDALTIWKKNKLWKY"
     gene            3581..5167
                     /locus_tag="VAH76_001892"
     CDS             3581..5167
                     /locus_tag="VAH76_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1386503.1"
                     /translation="MSAARQINNPKTRQWRILLVYNIYRIVSIFLFLGIYYFSYINKY
                     YPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLSGTIDIIAISSMLGIIGNLESG
                     YGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYHQKDLSTFFYSGIYG
                     AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRI
                     RLMNTAAREFFNLSKHHAIQKLDQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRV
                     HFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQ
                     LFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELITFLEHFKQ
                     DFTHNNPCDLTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAK
                     CSSNKITLTVCDSGPGIPSEHRDNIFEPFFTTLRGGTGMGLFIARDLCEINQARLNLV
                     KSNKGCCFAITVNPSDELLI"
     gene            5164..6492
                     /locus_tag="VAH76_001893"
     CDS             5164..6492
                     /locus_tag="VAH76_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEO1386504.1"
                     /translation="MTKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIK
                     QNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQVPVAVITAYGNVEGAVNTLKAGAFDYV
                     SKPIDLKMLRDLVKTALSMQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFI
                     QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDK
                     TGLFVAAHGGTLFLDEIAELPLAMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHK
                     NLIEEVNAGMFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKI
                     NPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPVVAVSPAS
                     TNLNGYLQDQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD"
     gene            6921..7166
                     /locus_tag="VAH76_001894"
     CDS             6921..7166
                     /locus_tag="VAH76_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386505.1"
                     /translation="MNSWNSVVFLKTKNAWSDSAAIAKMNGVQSLWSTSGDWDWCIKL
                     DSKHSSPEETALFVKNLRNADWVAETKTSWWKEVAVK"
     gene            complement(7463..8899)
                     /locus_tag="VAH76_001895"
     CDS             complement(7463..8899)
                     /locus_tag="VAH76_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEO1386506.1"
                     /translation="MIKLNLNYKLITKKTLPKLFYWGLGIVFFFLAPTTNAGSLSPYT
                     TKELEQLEREFIQLINQSDSVERNPLASQYINSIGKKLAHYGRIKTPYFFIVKSKEIN
                     AFAGPGGYIGINTQLILTSSNESELAAVMAHEIAHVRLHHLYSIIEHQKQMRVPLLAS
                     ILASAALGVLNPTVGMGAMMASVSGFSQDNINFTRSKEKEADRIGIDMLIKAGFNPQG
                     MAAFFNKMQENSRYYYTANIPAILRTHPLDADRIAEAENRTSRLPKKHYTDSLDYALF
                     KELIRVSVASNNKQILDYYEHHCHKKNPATACQYGYVLALLNNNNFQKAAEKLNPLLL
                     QDNSNLNFQIAMAQAELGLSQYQSALSRLAELQNNFPDNYAALMAYAQGLLTANQPEK
                     ATAVLLKGSRQYKEDLPLCRELARAQAESHQKSYAYFTQAQCLLLEGQKRGAIAQLKV
                     AQKLAKKDAYLSARISAKIDEIKFMVEN"
     gene            8962..10035
                     /gene="ribD"
                     /locus_tag="VAH76_001896"
     CDS             8962..10035
                     /gene="ribD"
                     /locus_tag="VAH76_001896"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO1386507.1"
                     /translation="MHEQFLLAALEQAKLGRGFCAPNPSVGAVAVQNGNIIAQAWHRG
                     AGTPHAEQLLLSQIPPQTPEVTLYITLEPCNHWGKTPPCVDAIINHGIEEVVFAYFDP
                     NPIVAKSNSSEKLREHGIQVRHIPIKAIDAFYKSYAYWTMTHKPRVTVKIAQTFDGKI
                     GKSEGDRVVLSNVLCEQFTHQMRAASDVILTSAKTVQMDNPRMNARLDSGVVAKPVAI
                     VDRNLSLSDDTIIFSTAKHCHIYHQPQANQAKTYPNSTHYAMPLQNGLMDLDAIITHL
                     GQLGYHDVWVEAGGAIFSALHSKGLVHRTYLYLVPGSLGHQAISAYQQQGIFEKAHTV
                     SWQAMGDNMIACLDWQEDECLPD"
     gene            10020..10634
                     /locus_tag="VAH76_001897"
     CDS             10020..10634
                     /locus_tag="VAH76_001897"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO1386508.1"
                     /translation="MFTGLIENTGKVLVNRQGENSSRLVISSSYQDLKPGESIAVNGV
                     CLTLLPADLPDLCFDISPETLHVTTLSKLSSGDMVNLERAMLASTRFGGHYVSGHVDT
                     RAKVKSIRAVNEFIAMDLEGFIPEHNHYLIPKGSITVDGVSLTINTVSEQTVGLMLVP
                     HTLLMTNLGSLRVGQQVNIEFDYLTRIVAHQLQIIGKLNHEEVP"
     gene            10631..11839
                     /locus_tag="VAH76_001898"
     CDS             10631..11839
                     /locus_tag="VAH76_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO1386509.1"
                     /translation="MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATP
                     EAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNKSPYGTAFTVSIEAANGVSTGIS
                     ARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
                     AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPL
                     EENGVFTMTVFENQIDSGEHFVLVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQ
                     LELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTVDANLELGLPADDRD
                     YAVAYQILKYLGVDALRLLTNNPLKIASLERYGIKITQRIPLEIKPSRENHSYLKTKK
                     IKLGHLLAID"
     gene            11847..12314
                     /locus_tag="VAH76_001899"
     CDS             11847..12314
                     /locus_tag="VAH76_001899"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO1386510.1"
                     /translation="MREIKTIVSDGVTFPIALVVSTFNELITSALKKGALDRLIELGF
                     KSEDITLVEVPGAVEIPFVAQLLAKKQKVEVIVALGAVIRGETSHYDYVCDQVSQGCQ
                     RVMLDYNIPVIFGVLTTENDEQALARVGGTHGHKGRDAIDCAVSMRSIKQQLQ"
     gene            12440..14077
                     /locus_tag="VAH76_001900"
     CDS             12440..14077
                     /locus_tag="VAH76_001900"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO1386511.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTNDGAETDLDLGHYERFVKTTMTKRNNFTSGKIYENVIKKE
                     RRGDYLGGTVQVIPHITNEIKRCIKLGADAFDVAMVEIGGTVGDIESLPFLEAIRQMR
                     IELGSQRAIFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDVLICRSEKPLSMA
                     DRAKIALFTNVEKEAVISLEDANSIYQIPMILHAQHLDEIVVKKLSLEAKQADLSEWQ
                     RVVDMQAVQTMTVKIAMVGKYTELNDAYKSINEALLHAGIHTETKVEIIYFDAEMIEK
                     HGALLLESIDAILVPGGFGERGVEGKIKAIQYAREHKVPFLGICLGMQTAVIEFARNV
                     VGLTGANSTEFNKETLYPVLGLISEWMDADGSKQIRDENTDLGGTMRLGGQYCHLAEG
                     TLARKVYGKPQIIERHRHRYEVNNKYVDSLVKHGLIISGRSADNSLVEMIELADHPWF
                     LACQFHPEFTSNPRDSHPLFKEFVLAARIHHQEKDKK"
     gene            14074..14898
                     /gene="kdsA"
                     /locus_tag="VAH76_001901"
     CDS             14074..14898
                     /gene="kdsA"
                     /locus_tag="VAH76_001901"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEO1386512.1"
                     /translation="MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLN
                     IPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSS
                     VVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
                     CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALA
                     RAAVAVGISGLFMETHPDPDKALSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF"
     gene            15103..>15246
                     /locus_tag="VAH76_001902"
     CDS             15103..>15246
                     /locus_tag="VAH76_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386513.1"
                     /translation="MPGIKKFNLTYQQVPKIFSLPESVFLSKDVELIPEEVLKQNKIQ
                     KNIV"
BASE COUNT         4677 a         2546 c         3319 g         4704 t
ORIGIN      
        1 tattataatt ttattgatgg aatttttctt atctcgcaac gaagcatatg cagaagctgc
       61 gacagatcct gagtagacat aaccagcatg cggaactagt attgcttttg gagcaggttt
      121 atgaattggc gcttggtcca ggaaattcaa tacggtttgc tttaaaatca atggatcgtc
      181 gggataaaaa tacccagcta cggctggttg acggacgctc acaacaacac tccggaacaa
      241 ctgaacatac tatacttata aatatagact aagagttgat tgtgggctat ttgatgtttg
      301 atactattcc aaccaaatac tggcataagc tggaagatgg gcgcatacaa tgtgatgtat
      361 gtcctcgtaa ttgtaaattg cgtgaagggc aaagagggtt atgttttgtt cgtgcaaacc
      421 tggataatca aataaaatta actacctatg gccgctctag cggttattgc atagatccca
      481 ttgaaaaaaa accattgaat cactttttgc cgggtagccc tgttttatct ttcggaactg
      541 cggggtgtaa tctagcctgt aagttttgcc aaaattggga tattagcaaa tccagagata
      601 ttgatacatt ggcagatact gccatgccag aagatattgc aagggctgcc caaaatatga
      661 actgtctcag tgtggcatat acatacaatg atccggtgat ttttatggaa tatgccattg
      721 atgtcgcaaa ggaatgtcat aagcttggca ttcattcagt agcggtctct gcagggtata
      781 ttaatcctga gccatgtaag gagctttatt cattcatgga cgctgctaat atcgatttaa
      841 aatcctttag tgaagatttt tattacaaaa ttactggttc tcatttacaa cctgttctgg
      901 atacgctcat ttatttgaag aaggaaacca gcgtatggtt tgaaattact aatttagtga
      961 ttccagaaga aaatgattcg gatgccgagt ttgagaggat gtctgaatgg attatgacta
     1021 acctggggcc tgatgttcct ttacacttca ctgcgtttca tccagattgg aaaatgatgg
     1081 ataaagcccc gactccattt tcaactttac aaagagccag agaaatcgca atcaagacag
     1141 gcttacatta tgtatatacg ggaaatgttc acgatacaac cggcagcagc acttattgtc
     1201 ctcattgtaa attacctgtg attgtaaggg attggtatcg aatattggat tatcgggtgg
     1261 atgatactgg acattgctta aattgtcata cgcaaattgc aggagtattt aagggggcag
     1321 ttggtcattg gggcccaaga agagtaatgg ttaaattagg ttgctcttaa tcttcccata
     1381 ataaattttt agtattatat gtcatgtttt tgtacatggt gaattaatat gagatttcaa
     1441 gattctgact ttgaagaacg ttataataca atgtggaata aaattgcagt atctgctgat
     1501 gctcaaataa ggcaattatt tggcgccaaa ggatttttta gtgaacaaca gcccaattac
     1561 tatcaactcc ttgtaaacta tgctcaagcg gccaaaaata ttgtcgataa tttaaatcgt
     1621 caatctccca tgtttgatga caaagaatat gttgaaggct atatgattgc tactctccaa
     1681 tcggtatata aagatttttc tcaatataaa ccgagaattg cagggcgtta tggagagcac
     1741 tcaagctgtg ttgaactgat taacaagacc ttggattggg tacaatcctt tgacttaaaa
     1801 ttagaaaacc tctctgaatc tgataatgag atgaaaatta ctttctaatt gcaagctatc
     1861 ctgtattacg aaaagtctgt aatgcgggat agcatcattt ccctattata tcttactcct
     1921 aataggtgca ctttgcttgt atggcaccag agcgtcaatg tttattcatc atcaattgtg
     1981 ctataattga acaatgattg cagggaggta tgatgaaaca attggttgtt gcaggatttt
     2041 tcttattact tctaacttcc cctttatcag cgctggacgt tgatacctat cgtagttcac
     2101 aaaaacaatt caatacagag gataaggcgg aacagggtga tgctcaagca cagtttgaat
     2161 tagggctcca atatgaaaag ggggatggag taaaaaaaga tttaaagaaa gcgatttatt
     2221 ggtatcagaa agccgcagat caaggacaag ctgaggcaca aaataacctc ggcgtattgt
     2281 atcttaaagg agaaggtgtg ccccaaaaca gtcagcaagc aatgtattgg tttaagaagg
     2341 cttcggaaca gggtttggca attggtcaaa ataatatagg gatcctatat gaaaatggtc
     2401 ttggtgttaa aaaagatctg ggccaggcgt ttatctggta tcaaaagtct gcagaaggcg
     2461 gaaattcaga cggacaatat aaccttgcag tcatgtacat gtatggcaat ggtataccaa
     2521 aagatatcaa aaaggcgatt cactggtata tcaaagcggc agagcaagga gatcttgatg
     2581 ctcaaaataa tcttggtgtt ctttatgagc gaggcgaaga ggttccaaga gatttaaaag
     2641 cagctattag ttggtatacc agagcagcaa atgaaggttc actgattgct caaactaacc
     2701 ttggtgtatt gtatatgact ggtgatcctt ccattcaaga tggcaagaaa gcaatatatt
     2761 ggtatgaaaa agcagcggca caaggtgggg aaaaagctca aaataatctt gggtatattt
     2821 atgaacaagg aattggtaca gaaaaggata tgaaaaaggc tatttactgg tacgaaaaag
     2881 ccgcagagaa tggatttact ctggctcaaa ataatttagg tgttctctat tcaaatgatg
     2941 gcgagctgca agattacaaa aaggcttatt tatggttcaa aaaagccgca gatcaaggat
     3001 ttgccgaagc ccaaaataac ttgggtttaa tgtatatgaa aggcaatgga ttgtcagtta
     3061 attatcacga ggcagtcttg tggtacaaga gagcggcaga acaaggatta cctttagcgc
     3121 aacataatct ggcgattatg tatatgaaag ggttggggat aaagaaagac aataagctgg
     3181 ccattaaatg gtaccaaaaa gctgctgaaa aaggattgga tttggcgcaa aacaatttgg
     3241 cggttatgta tatacgaggc gaaggagtga aaagagattt taagaaggcc atgtattggt
     3301 atcaaaaggc agctgaacaa ggtttggatt tggcacaaat taatctgggt attatgtatc
     3361 ttgatggcat gggtgttaat aaagatttcg ctaaagcaaa atactggata ggaaaagcca
     3421 aggatagtgg ttctcaagat gcgctgacca tatggaaaaa aaataaattg tggaaatatt
     3481 agtgatcatt gttgtgggtc ttggttgatg tattgcacaa aatgatcaag tctggttata
     3541 taattaaaat acgctgaata atctaatgga ttagaaaagg atgtctgctg ctcgccaaat
     3601 caacaatccg aaaacgagac aatggcgaat attgttggtt tacaatattt acagaatagt
     3661 aagtattttt ctatttttag gcatttatta tttcagttat atcaataaat attatccctt
     3721 attatttctc tgtatcgctt ttgcctattt agtatttgct ttgatttttc tttatttttg
     3781 gcatagaaaa attttaagtt ttgataagca ggtgttttta tcgggtacga tcgatatcat
     3841 agctatttcc agcatgttgg gcatcattgg taatttggaa tccggttatg gcatattact
     3901 aaatgtgact attgctgctt taagtatttt agttcccggg cgcttggcca tctattttgc
     3961 atcactagcc agttgtttat tgctgtgcgg caatgtgttg caatttttta tttatcatca
     4021 aaaagattta agcacttttt tttatagtgg aatctatggg gctggttttt ttgcaacggc
     4081 tatcactgcc tggtatttat ccaattgggt cagaatgtcg gaaagtctgg ccagacaccg
     4141 tagttatgaa ttggcaggaa tgcaaaggct caatgaatat attgtcgaac gattgcattc
     4201 aggaattatt tatgttgcag aggggaagcg aattcggttg atgaatactg ctgcccgtga
     4261 attttttaat ctgagtaaac atcatgcaat tcaaaaattg gaccagctat ctgattcact
     4321 tgtatccaaa tttgatgatt ttcttttaaa aacaacgaat aatgaacgta cagctcagac
     4381 tattattgaa gatccttatc ttagagtgca ttttttttct acttctgttg gtcacaagtc
     4441 tgaagtactc attattctgg aagacatgac ttatattgca caacaagcac aacaactcaa
     4501 attagcggct ttagggcgtt tttcggcaag tattgctcat gaattacgta atcccttggg
     4561 ggcaattgct catgctattc aattgtttgg agataatgag gatttaaatg aagagaacac
     4621 tcgtttaaaa caattaatca tgaacaattg tgagcgaatg aacagggtga ttaaaaatgt
     4681 attgcagctt tcaaggcgtg agaaatctca accgcaggtt aatgaactta taacattttt
     4741 agaacatttt aagcaggatt ttactcacaa taatccctgt gaccttacga taaaactacc
     4801 tgtaaataag ccgttatcag ttgtttttga taaaagtcaa ttagaacaga ttttagtaat
     4861 cttgtgtgaa aatgctattc aacatgggcg ggatcatgag ggtaatgtga atatcgtcat
     4921 tgctgccaaa tgttcttcga ataaaatcac tttgactgta tgtgattctg gacctggtat
     4981 accctctgag cacagagaca atatatttga accatttttt accaccttgc gaggtggtac
     5041 agggatgggg ttatttattg ccagagattt gtgtgaaatt aatcaagcca gattaaattt
     5101 ggtaaaatca aataaaggat gttgttttgc gatcactgtt aatccatcgg atgaactttt
     5161 aatatgacca aaagtaaagt gcttgttata gatgacgaac cagatataag agagttatta
     5221 actcttactt tatcgcgtat gggtttagct tgcgatgcag cgtctgactt taagcaaggt
     5281 gtagaacata taaaacaaaa ttattattct ttggttttaa ccgatatgcg tttgcctgat
     5341 ggagatggta tagaaattgt taaatttatt cagaaataca aaccacaggt gccagttgct
     5401 gtcattactg cctatggaaa tgtcgagggt gcagtcaata ctttaaaggc gggtgcattt
     5461 gattatgtat caaaacctat agacttaaaa atgcttagag atctggttaa gacggcatta
     5521 tccatgcaaa acccgtctac cgagaggcct gatgagagtc tattggttgg tgaaagtttg
     5581 gtcatgcaac aacttcgtga aagcattcat aaattagctc gcagtcaggc accggttttt
     5641 attcaagggg attcaggtgt tggaaaagaa ctggtcgctc agttgatcca tgctaatggt
     5701 cccagaaagg ataagccatt tattcctgta aactgtggcg ctatacctgg tgagttgatg
     5761 gagtcagagt tttttggcca taaaaaaggg agctttacag gagcgatatc tgataaaaca
     5821 gggctgtttg ttgcggctca tggtggcact ttgtttcttg atgaaatcgc cgaacttcct
     5881 ctcgccatgc aggttaaatt gttacgtgct attcaggaga aggcaattaa gccgataggt
     5941 gagttatatg aagttcctgt ggatgttcgc atcctaagtg cgagtcataa aaatttgatt
     6001 gaagaagtta atgccgggat gtttcgtcaa gatttgtatt atcgaattaa tgtgattgaa
     6061 ttgcgtgttc caactttaat ggaaaggctt tcagatattc cttgcctgac agaggcgata
     6121 ttaagaaaat tatctaaaaa tcaaaataga aacctggtga aaataaatcc atcctgtttt
     6181 gaaatattga gccgttatca ttttccagga aatgtcagag aactggagaa tattctcgag
     6241 cgtgccatgg ctatgtgtga aggagaggtt atagaaccat ccgatttaca tttgcccaga
     6301 acaacaataa ccccagttgt tgccgtatcc cccgcgtcga ctaacttaaa tggttacctt
     6361 caggatcagg aaaaagagtt aattcttgat gcgcttgaga aaacaaaatg gaacagaacc
     6421 gctgctgctc aactactggg ggtcagcttc cgaactctcc gttatcgatt aaaaaaactg
     6481 ggtcttgatt agatgttgcc ctttgaagat tttcaaaggt taacatagat ctcctgcctg
     6541 gccagcctct tttgttttat tgctgccctg aaaacgtttc tgaggtgctc atttactgta
     6601 tcaggactta tgaaaaaccc caatctctat gttaaaaatg tttttaattc ggtcatttag
     6661 tttatctaaa ctcccacatt aaaaacattt tttgccttga tcttggagtt tttcttaagt
     6721 cctgtgtatg taaactctgt tcctcaaaac gttttcgcct tgccgtgaaa caaaataatt
     6781 agcttaggca gaaggtctaa tatcagaatt gtttttcatt cgagtttgaa atctgataat
     6841 tcatagtcta attttatata acgcaatgaa tttatgttca atgcgaaaaa acataattga
     6901 ttaaaattaa ggaagaaaca atgaactcat ggaacagtgt tgttttttta aaaactaaaa
     6961 atgcctggtc agattctgct gcaatagcaa agatgaatgg tgttcaaagt ctttggtcta
     7021 cctctggaga ctgggactgg tgtataaaac ttgactccaa gcactcaagc ccagaggaaa
     7081 ctgccttgtt tgtcaagaat ttaaggaatg ccgattgggt tgcagaaacc aaaaccagtt
     7141 ggtggaaaga agttgctgta aagtaaatta atgagatgag tttcttggga aattataatg
     7201 gtaattacca ttataatttc ccgctggatt atgagtaatt ttaaggtaaa cacttctgcc
     7261 atactatttt acttcagctt tgcgcctcta tttgcttttt gatggagact ttctattttc
     7321 tttcatttct cagacataac agaaataagg ctcttgcaag cattggttga ttacattgga
     7381 cttggataaa gaaaaattaa tcccttatgc tcttgccgaa tggtatatga gtttatttta
     7441 ttttaattaa tctatattta gattagtttt caaccataaa ttttatttca tcaatttttg
     7501 ctgaaattct tgcggataaa taagcatcct ttttggccag tttttgcgct acctttaatt
     7561 gagcaatagc tcctcttttt tgcccctcaa gtagcaagca ttgtgcttga gtaaaatagg
     7621 cataactttt ctgatgggat tcagcttgag ctcttgccaa ttctcggcaa agcggtaagt
     7681 cctctttgta ttgcctacta cccttaagaa gtacggctgt agctttttca ggttgatttg
     7741 cggttaacaa tccttgggcg tatgccatta gagcagcata attgtcagga aaattatttt
     7801 gtaattcagc aagacgggag agtgctgatt gatattgtga taaacctaat tcagcttgag
     7861 ccatagcaat ttgaaaattg agattactat tatcctgtaa cagtaaaggg tttaattttt
     7921 cagccgcttt ttggaaattg ttattattta acaaggcaag tacgtaccca tactggcaag
     7981 cagtggcggg atttttttta tggcaatgat gctcataata atcaagaatt tgtttattat
     8041 tagaggcgac agaaactctg attaactctt taaataaggc atagtccaaa gaatcagtat
     8101 aatgtttttt aggcaaacgg ctggttctgt tttcagcctc agctatcctg tcagcatcca
     8161 gggggtgagt ccgcagtatt gctggaatat tggcagtgta ataataacgg gaattttctt
     8221 gcattttatt aaaaaatgcg gccatacctt gaggattgaa gcctgcctta ataagcatgt
     8281 ctataccaat tctgtcggct tctttttctt ttgagcgtgt aaaattgatg ttgtcctggg
     8341 aaaatccgga aacggatgcc atcatagcgc ccatcccgac agttggattc aagacgccca
     8401 aggctgcaga cgccagaata gaggctaata atggaacacg catctgtttt tggtgttcta
     8461 taatgctgta aagatgatgt aagcgaacat gcgcaatttc atgagccatt acagctgcca
     8521 gttcactttc attgctgctc gttaaaatga gttgggtatt aatgcctatg taccctcctg
     8581 gacctgcaaa tgcattgatt tctttggatt taacaataaa aaaataaggt gttttgatac
     8641 ggccataatg tgccagtttt tttcctatgg agttgatgta ttgactggcc aaaggattgc
     8701 gctctacgct gtccgattga ttaataagtt gaataaattc tctttctaat tgttccagtt
     8761 ctttggttgt atagggtgaa agacttccgg cattagttgt aggtgctaaa aaaaagaaga
     8821 cgatccccaa accccagtaa aatagtttag gtaatgtctt tttcgtgatt aacttataat
     8881 ttaagttcaa tttaatcatt aggctactca ataattgaat aatttgttat cattcgacca
     8941 cattaagaag gtggttaata tatgcacgaa cagtttctgc ttgcagcact tgaacaagcc
     9001 aaactgggga gaggtttttg tgcccccaat cccagcgttg gggcggttgc tgtgcaaaat
     9061 ggtaatatta tagcacaggc atggcataga ggtgcaggta caccgcatgc agaacaatta
     9121 ttgttatcac aaattccacc ccaaacgcca gaggttacgc tctatatcac gctggagcct
     9181 tgtaatcact ggggcaaaac tcccccttgc gttgatgcta ttatcaatca tggcatagag
     9241 gaggttgttt ttgcctattt tgacccaaac cccattgttg ccaaaagtaa ctcctcagaa
     9301 aaattacgtg aacatggaat acaagtcagg catattccta taaaggcaat tgatgcgttc
     9361 tataaaagct atgcttattg gactatgacc cataaaccaa gagtcactgt taaaatagca
     9421 caaacttttg atggtaaaat tggaaagtct gagggagatc gagtggtttt gtccaatgtt
     9481 ttatgcgaac agttcactca tcaaatgcgc gctgccagtg atgtcatatt aaccagtgcc
     9541 aagacagttc aaatggataa tccaagaatg aacgccagat tagatagtgg agtggtagca
     9601 aagccagttg ccattgttga ccgcaattta agtttgtctg atgacacaat cattttttcg
     9661 acagcaaaac attgccacat ttatcatcaa ccacaagcaa atcaggccaa gacttatcct
     9721 aatagcactc attatgcgat gcctttgcaa aatgggttga tggacttgga tgcaataata
     9781 actcatcttg gtcaactggg atatcatgac gtatgggttg aagcgggcgg agctattttt
     9841 tctgcgttac atagcaaggg attggtgcat agaacctatt tgtatttagt tccgggcagt
     9901 ttagggcatc aagcgatttc agcttatcaa caacagggga tttttgaaaa agctcatacg
     9961 gtgagttggc aagctatggg tgacaatatg atagcatgtt tggattggca ggaggatgag
    10021 tgtttaccgg attgatagaa aatacaggca aagtattagt gaataggcaa ggagaaaatt
    10081 ctagtcgttt agtcatttct tcaagctatc aggatttgaa acctggtgag agtatcgctg
    10141 taaatggggt ttgtttaacc cttttgccag cagatttgcc tgatctgtgt tttgatattt
    10201 cacctgaaac tctgcatgtt accacattgt caaaattatc atctggcgac atggttaatc
    10261 ttgaaagagc tatgctggca tctactcgct ttggtggtca ttatgtatca ggccatgtag
    10321 acaccagggc caaagtaaaa tccattcggg ctgttaatga atttatcgcg atggatttgg
    10381 agggttttat cccagaacac aatcattatt taatacccaa aggcagcatc acagtagatg
    10441 gcgtcagctt gactattaat actgtatcag aacaaaccgt cggactcatg ctggtgccac
    10501 atacattatt aatgaccaac ctcggttcat taagagtggg ccagcaagtc aatattgaat
    10561 ttgattatct tacccgtatt gtggctcatc agcttcaaat tatcgggaaa ttgaaccatg
    10621 aggaagtgcc atgaaacatt cattagcaac aatagaagag gctgtggcga cacttaaagc
    10681 cggtaaaatg attattctaa tggatgatga agatagagag aatgaaggtg atttggtgat
    10741 tgctgctgag cacgctactc ctgaagccat taattttatg tctcgtcttg gttgtggttt
    10801 aatttgtttg cctatggctg aagaattgat agacaaactc caactgccca tgatggctcg
    10861 gcataacaaa tcgccgtatg gaacggcgtt tacggtttcg attgaagcag ctaacggtgt
    10921 ctccacaggc atctcagcaa gagacagagc tcgaacaatt caagtggcta ttgatccaaa
    10981 aagtgggcca tctgatatca tttcacctgg acatgttttt cctttacggg ctagaaagag
    11041 aggagtttta gaaagaccag gtcaaacaga aggtagtgtt gatttggtaa gacttgccgg
    11101 attgaccccg gcggcagtga tttgtgagat cattaatgaa gatggaacaa tgagcagacg
    11161 tgatgaatta gctctttttt caaaaaaaca tcaaatacct ttagtcacca ttaaagattt
    11221 gattaattac cgaattcgtc acgaaaatct tattaatcct gtcgcaacca ctcagattcc
    11281 tctggaggag aatggtgttt ttactatgac tgtttttgag aatcagatag attctggtga
    11341 acattttgtc ttggttaaac cccctttgta tggtaatcaa gtgcctttgg ttcgaattca
    11401 ttctgagtgc ataacaggtg atgtttttgg ctccagaaaa tgtgattgtg gaaaacaact
    11461 ggagttgtct ttatcccaga tcggagctga aggcggtata ttaatttatt tgcgtcagga
    11521 aggccgtgga ataggtttgg caaacaaatt aaaggcgtat gctttgcaag agcaggggtt
    11581 tgatacagtc gatgctaatc tggaattagg attgcccgct gacgatagag actatgccgt
    11641 agcataccaa atactgaaat atttgggggt tgacgcatta aggttgctga caaataatcc
    11701 tttaaaaatc gcttctcttg agcgttatgg aatcaagatt actcaacgca ttcctttgga
    11761 aattaagccc tctagagaaa atcacagtta tttaaaaacc aaaaaaataa agctggggca
    11821 tttattagcc attgattagg ataaacatga gagagataaa aacaattgtt agtgatggtg
    11881 tcacgtttcc aatagcgcta gtagtaagta cttttaatga attgataacc tctgcattaa
    11941 aaaaaggggc gttagatagg ttgattgaac tgggttttaa atcggaggac attactttag
    12001 ttgaggttcc tggcgcagta gagattccgt ttgttgcaca gttgctagcg aaaaaacaaa
    12061 aagtagaagt catagtggca cttggagcag ttatccgtgg tgaaacctct cattatgatt
    12121 acgtttgtga tcaggtcagc cagggatgcc aaagggttat gttagattat aatatcccgg
    12181 ttatctttgg tgtattgact actgaaaatg atgagcaggc tttggcaaga gtaggaggaa
    12241 ctcacggcca taaagggaga gatgcgatag attgtgcggt ttccatgcgt tctattaaac
    12301 aacaattaca atagttctat taattttgct caaatttctg ttatttatat tttcattttg
    12361 atagaattga atcccgttta catgcaaatt aaattgaaca ccaaattggt ggattcggca
    12421 ttttgagcgg ggtcaaaaaa tgacaaaata tatttttatt actggcgggg ttgtgtcttc
    12481 tttaggaaaa ggcatagcag ccgcatctct tgcagctatt cttgaagctc gtggtcttcg
    12541 cgttacgttg ataaagcttg atccctacat taacgttgat cctggtacta tgagtccctt
    12601 tcagcatggc gaggttttcg taacgaatga cggggcagag accgatttgg atttggggca
    12661 ttatgagcgc tttgttaaaa caacaatgac caagcgtaat aatttcacat cgggaaaaat
    12721 ttacgaaaat gtaattaaga aagaaagacg gggtgattat ttaggaggga cagttcaggt
    12781 tattcctcat attactaatg aaatcaaacg ctgtatcaaa ctcggtgcgg atgcattcga
    12841 tgtggctatg gttgaaatag gaggaactgt tggtgatatt gaatcactac cctttcttga
    12901 agcaattagg cagatgcgta ttgagttagg ttcacaacga gcaatattta ttcatcttac
    12961 tttggttcct tatattgcta cttctgggga aaccaaaacc aaaccaactc aacattcggt
    13021 aaaggaattg cgttctattg gtatccagcc agatgtattg atttgccgtt cagaaaaacc
    13081 attatccatg gcagacaggg ctaaaatcgc attattcact aatgttgaaa aagaagctgt
    13141 gatttctctt gaggatgcga atagtattta tcaaatacct atgattctac atgcgcagca
    13201 tttagacgaa attgttgtta agaaactaag cctggaagct aagcaggctg atttgagcga
    13261 gtggcaacga gtagttgata tgcaagcagt tcaaacgatg acagtgaaaa tcgctatggt
    13321 aggtaaatat acggagctga atgatgccta caagtcgatt aatgaagctt tattgcatgc
    13381 tggaattcat acggaaacaa aagtagaaat tatctatttt gatgctgaga tgatagaaaa
    13441 gcatggcgct ttattgcttg aatccattga tgctattttg gtacctggtg gttttggtga
    13501 gagaggagtc gaaggcaaaa ttaaggctat ccaatatgct cgggagcata aggttccttt
    13561 tttagggata tgtttgggta tgcaaacagc tgttattgag tttgcgagaa atgtagttgg
    13621 tttaacagga gccaattcga ctgagttcaa taaagaaaca ttgtatccag tgcttggttt
    13681 aattagtgaa tggatggatg ccgatggtag caagcagatc agggacgaaa atactgactt
    13741 gggtggaacc atgcgcttgg gcggacaata ctgtcatctt gctgaaggca ctctggcaag
    13801 aaaagtttat ggcaaaccac aaattataga gcgtcatcga catcgatatg aagtcaataa
    13861 caaatatgtg gatagtttgg taaaacacgg cttaattatt tcaggacgat cagcagataa
    13921 ttcactggtt gagatgatag aattagcgga ccacccctgg tttttagctt gtcagtttca
    13981 tcctgaattc acatcgaatc caagagatag tcatcctttg tttaaggaat ttgtacttgc
    14041 agctcgtatt caccatcaag agaaggacaa aaaatgagat tatgtggttt tgaagcaggc
    14101 ttggataagc cattgttttt gattgccggc ccttgtgtta ttgaaagtga agaactggct
    14161 ttagagaccg caggatattt aaaggaaatg tgcagtcaat tgaatatccc tttcatctat
    14221 aagtcttctt ttgataaagc aaaccgttca tctatttcca gttacagggg gcctggcttt
    14281 gaaaaagggt tatcgattct ggaaaaggtc aaatcacaaa taggtgttcc tgttttgact
    14341 gatgttcatg aagacacgcc cttatttgaa gtctccagtg tggtagatgt attacaaacg
    14401 ccagctttcc tatgcagaca aaccaatttt atccaaaaag ttgctgctat gaataagccg
    14461 gttaatatta aaaagggaca gtttcttgct ccttgggaaa tgaaacatgt gattgcgaag
    14521 gcaaaggcgc aaggcaatga gcaaattatg gcttgtgagc gaggagtgag cttcggctac
    14581 aataatttgg tttctgatat gcgttccctc gttatcatgc gtgaaaccgg ctgtccagtg
    14641 gtttatgatg caacacattc ggttcaattg cccgggggca ataatggtgt atccggaggg
    14701 caaagggaat ttattccggc tcttgcaaga gcagctgtag ctgttggaat ttcagggtta
    14761 tttatggaaa ctcaccctga tccggataaa gcactaagtg atggtcccaa tagttggcct
    14821 ctagataaaa tgaaacaact tttggaatca ttgaaagcag ccgatgaagt gtataaaaag
    14881 tacagtactg atttctaaat taaatgatat cccgtgtaaa aagaagtatg gcacgggaat
    14941 attttttatt ttgctcctcc cttttatctg attaatcata ccgtttttat ttgattcaaa
    15001 agagataata atggtattaa tataatcaca ctgatcccaa tgttttcttg atgaataaaa
    15061 tgttacaata tgtgcattga gaaaatattg gagtcagtca ttatgcccgg tattaaaaaa
    15121 ttcaatttaa cttatcagca agtacctaaa atattttcat tgcctgaaag tgtattttta
    15181 tccaaagatg ttgaattaat tcctgaagaa gtattgaaac aaaacaaaat acaaaaaaat
    15241 attgtt
//
LOCUS       DATNSN010000080        15206 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.080, whole genome shotgun sequence.
ACCESSION   DATNSN010000080 DATNSN010000000
VERSION     DATNSN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.080"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..334)
                     /locus_tag="VAH76_001903"
     CDS             complement(<1..334)
                     /locus_tag="VAH76_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386514.1"
                     /translation="MTFTPPEFKILSVNTRNLETIFSTLLGRYKIITDPPVSEAVSSG
                     EIIRNTLETLLARTHKVVICKTDRETARDVFKQLSNELREVLKENNEEKNKQAILFLL
                     GALLHRYFR"
     gene            complement(347..994)
                     /locus_tag="VAH76_001904"
     CDS             complement(347..994)
                     /locus_tag="VAH76_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1752 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386515.1"
                     /translation="MRFLRVGPCGRETMLEFAEFQQNALKLTDQLKKRSIPSLGCFCF
                     FSVILPNTAAIEDTHTIKLVAYSLDSENDDDLIAYLKGVTRYSAVTPKNINEFKKKVL
                     TGIYLMMWSHYSSTVSNLMNSTLVELFQRDLQVSSPQKMSDTLFDASLEALSQYCSFA
                     YQNRNERIYDNLIRRLGKTVQADIHALRHSREKQETSFYDIYKGIMRTLGLNSVF"
     gene            complement(1287..2654)
                     /gene="mpl"
                     /locus_tag="VAH76_001905"
     CDS             complement(1287..2654)
                     /gene="mpl"
                     /locus_tag="VAH76_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEO1386516.1"
                     /translation="MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLL
                     AAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENIL
                     SKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
                     ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGV
                     AIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAELIDDSGSQFKILHNGVMVAEVS
                     WPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGVTVYDDF
                     AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEP
                     QDFNLKDFVSSWICPYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSI
                     DKLFM"
     gene            complement(2656..3276)
                     /locus_tag="VAH76_001906"
     CDS             complement(2656..3276)
                     /locus_tag="VAH76_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386517.1"
                     /translation="MEEYKIFCLVQRVLKPKENNQKIIMNTLKLLFSYYWRLSLLKET
                     PENSPYSVFLLVVMCILFSIIMLIQWNIAELDFSRDWLLSLMMATSLILSFIFYTYVI
                     LKFQNLASRLVQTSTCLLSAYIIIHVLATPLFLIDTYLSAENLKTPIFLFIGMIYLFI
                     TLGLSIWQFVITAHIYKFALGTTPVKSVLAAFGLVAVNILTISFWR"
     gene            complement(3329..7150)
                     /locus_tag="VAH76_001907"
     CDS             complement(3329..7150)
                     /locus_tag="VAH76_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HEO1386518.1"
                     /translation="MTDNNLLANKFFKKIWMVLAVLIIVAAVFSSVFRALTPWAKQYK
                     GEVEQHLTSLLGQPVTVQTMETGWYWFEPVVKLKHIRIHDGHNKSIYLDRLIVGVNLF
                     KSLWNWRIQPGVLYIDDMHLVFREKDGHWCIEGISRNAMKDTDITPEKTREILDWLAQ
                     QEKLIVRHVSAHLHFNDGSLIPVNELNLSIVNKGGNYKIKGEARLEQTNATVFQLLGN
                     VQFDPSQIKNTNGRFYFSAINVLPAQWQNLLPKSSMQLEGGKGNVSLWVDLEKGNISS
                     IQAELRLKRLAWKLLNKKNSKLIQSFYANMGWKLDKQGWQFQADHMKLRVAGVTWPEN
                     QLSIRFNKNQNTYQIFTKSLIVESLLSEAINWPPAIQNVLQLKPHGILSDVQVLIKEN
                     QINYILTRFDQLGWRAKDNIPGVSNLSGVLNWQPTEGRLELDSENSTFDIKGYPAQKL
                     TIFNGAFDWKELNDGLRLSIERLVASQPELTISLEGAIDQLTQNSVGNIRLVGNFSGK
                     NLQKWMPYLPKKHMKAKLFAWLNNDIKRLGQATGKITINGMAKDFPFDNGNGEFSIVS
                     HVVGGDILITPEWRLIKDLEGYIKLKNRNLDIDIIDGDAQGTLLKQMNLRIDDIGKDR
                     ETLLIHGIVHGKAQQMLNFVMNSPLKKKLSALKILLIKGMLSLNLRLEIPLYPENDDN
                     LARGELTFENNIINIKHQMGDFSVRDVTGNLLFNEKGIQDSALTARAFGYPLNIKIQS
                     EKSPQPLTAISIEGECTVESLKKKWHLPIFSVLKGNILAKALLKITDDPNDLDNMKLS
                     SDLIGLAVDLPAPLGKTYKEKTPLQVNLDFNPQKTLRLRVNYNGRLSTDLLMQKQKGV
                     FKLKSGQIRLGSAQAVNQDHPGLGIAGTLDGFDLQAWKEVINRFSKENVNPSLLSLVN
                     IIDVKMGKLNFLNQQFDNMSLYAKKRPDNDWSINLKQKKVDADLTYHSATNSLSGFVK
                     YLHLSEIGSSKNNKDTPSHTTPGQIPNLNLRVDNFSIGKIQIGNITLKSHSTSEQWKI
                     DYCRIDSPYYQANFEGEWLQKGKFNQTKLQSKMYLKDLAKSLDRWAINPAVDAGKGYI
                     EFHGGWQGSLYDFSLSKLNGSMYLQLKNGRITHLSPETEEKLGLGKLLSILSLQTIPR
                     RLKLDFSDLSNDGYSFDIFKGNFNVNNGIMSTNNSYIDGPVAYASMKGDLDLVRRLYD
                     LDLNISPHITASLPVVATIAGGPIAGLAAWIANKIINQSMQKIAGYSYKISGPWDQPI
                     VQQLNIVKKLP"
     gene            complement(7140..7595)
                     /gene="fliJ"
                     /locus_tag="VAH76_001908"
     CDS             complement(7140..7595)
                     /gene="fliJ"
                     /locus_tag="VAH76_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEO1386519.1"
                     /translation="MSDRLDRLIQLLKIKQEATQQAYIELVKAREQFNQNKARHEQLV
                     GYRQDYLQQLEVLGQQGSYVGPLRNRINFINHLDTALVQLNSYLSQLAKNRMKADLNY
                     KQAKTSEEGISKLIERVKRAELKQLQRIEQKETDEYAQKQWYSSLKHDR"
     gene            complement(7592..8941)
                     /gene="fliI"
                     /locus_tag="VAH76_001909"
     CDS             complement(7592..8941)
                     /gene="fliI"
                     /locus_tag="VAH76_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEO1386520.1"
                     /translation="MAIYQTQLHKILSGTRNKHHSGLLHCGRISRAIGLTIEAKGFNQ
                     PVGARCLVKINDEHSVQAEVVGFSNESVYLMSIGDIQGIAPGMKIIPTGRIAQVSVGH
                     QLLGRILNGSGMFIDDGPPGEMVETYPLIGQVINPLKRAPIEVPLDVGIRAINGLLTV
                     GRGQRIGLFAGSGVGKSVLLGMMTRFTEADVVVVGLIGERGREVKEFIECNLGLEGLR
                     RAVVVAAPADESPLMRLHGAKVATSIAEYFRDQGKHVLLLIDSLTRFAQAQREIALSI
                     GEAPATKGYPPSVFAKLPKLVERAGNGTSGGGSITAFYTVLTEGDDLQDPIADAARAI
                     LDGHVVLSRALAEQGQYPAIDLEASISRVMQGVVSEQHMKDMLLFKKYLSVYEKNKDL
                     ILLGAYAKGSDPLVDKAILAVNKLKDYLAQGMDEQVSYDESLSSLTKLISTLVGNDQ"
     gene            complement(8944..9582)
                     /locus_tag="VAH76_001910"
     CDS             complement(8944..9582)
                     /locus_tag="VAH76_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEO1386521.1"
                     /translation="MSNEFEPYNKEIKKIEFTTWKYQASKDAEHSPSDEEELINELEV
                     VKQEAFQKGYADGFQQAQEEINAIKAELSKWLDLIQKPVQILDEQLTQEIIQTIIWLS
                     QHCVAVELSVNPDKLNDLLNKIKGELPSLTGKKSISMHPDDVRWIKEVFSESNIPGLH
                     ELLVADPALNRGDFYLQGEHSELDGRIQTRFTTLFNEYINKENIIPMGVSQE"
     gene            complement(9724..10713)
                     /gene="fliG"
                     /locus_tag="VAH76_001911"
     CDS             complement(9724..10713)
                     /gene="fliG"
                     /locus_tag="VAH76_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEO1386522.1"
                     /translation="MDGIERAAILLLSMGEKNASEVLKHLEPRQVQKVGMAMSTMNSV
                     SKAKMQNVLIDFISAIEDQTSLTVDTEHYLRTVLIEALGEEKAIPFIDRILVSNKDSG
                     LNKLKWLDGRLIAEVIRNEHPQIIATILIHLDSEQSAEVVSYFSAEKRAEVLLRMCNI
                     DTVKPEAISELGHVIEKQLSGQTTGKSSSVGGIKTVADVINFLDGAMEEEVLERIKDW
                     DEELSEKIRDKMFVFENIKDMDDRSVQTLLREVTSDQLKLALKGTTETTKEKIYSNMS
                     KRAADLLRDDIEVQGPVRVSDVERAQRDILAIARNLAEDGKISLGAKGGDELI"
     gene            complement(10716..12350)
                     /gene="fliF"
                     /locus_tag="VAH76_001912"
     CDS             complement(10716..12350)
                     /gene="fliF"
                     /locus_tag="VAH76_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEO1386523.1"
                     /translation="MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSR
                     DPSYVPLYSQLNAQDSSEILTILERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGL
                     PRDTAIGHDLLGSSGGVFNTSQFMENARYRQALEAELARTISKFNNIKSARVHLAIPR
                     ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSAGRVTVVDQDGQL
                     LNEGSGYNLFSETERFLDYRQNLEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYE
                     QTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATP
                     NNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMND
                     KTKKLEKKPLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFW
                     QKDSFWSVIKQIGGALFILAILFGVLRPMLKTLASNKDMDQATDNQDTGDFTLDGKIS
                     FRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG"
     gene            complement(12522..12836)
                     /gene="fliE"
                     /locus_tag="VAH76_001913"
     CDS             complement(12522..12836)
                     /gene="fliE"
                     /locus_tag="VAH76_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEO1386524.1"
                     /translation="MSDINTVNLINQMKTLAAQAEGIKPDSGLNHASFSSVFQQALNQ
                     VNELSQTADSLKTRFEIGDPNVSLGEVMIASQKSNLGFEATLRVRNKLVQAYQDIMNM
                     PV"
     gene            complement(12851..14164)
                     /gene="fleR"
                     /locus_tag="VAH76_001914"
     CDS             complement(12851..14164)
                     /gene="fleR"
                     /locus_tag="VAH76_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor FleR"
                     /protein_id="HEO1386525.1"
                     /translation="MSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
                     NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQK
                     PFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGT
                     GKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQ
                     AQGGTLLLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEV
                     QAGRFREDLYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQK
                     AMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKGTSKNLQI
                     HEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV"
     gene            complement(14188..>15206)
                     /locus_tag="VAH76_001915"
     CDS             complement(14188..>15206)
                     /locus_tag="VAH76_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO1386526.1"
                     /translation="YSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSA
                     EAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLADGRRVHVAISSLESLPGILITLSD
                     ITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIKNW
                     IHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHV
                     NNHLIARESSIHGESLIGAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGM
                     SEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPCLN
                     RNVG"
BASE COUNT         4463 a         3301 c         2463 g         4979 t
ORIGIN      
        1 gtctaaaata gcgatgaagc aaagctccta ataaaaacag gatggcttgc ttattttttt
       61 cctcattatt ttcttttaaa acttctcgca attcattact taattgttta aaaacatctc
      121 tggcagtttc tctatctgtt ttgcaaatca caaccttatg ggtccgagct aataatgttt
      181 ccaaagtatt tctaattatt tcaccactcg ataccgcttc agatacagga ggatcagtaa
      241 taattttata cctgcctagc aaggtagaga atattgtctc taagttacga gtatttacac
      301 ttaatatttt gaactcgggc ggagtaaaag tcatagtgtc tcctgtctaa aacacgctat
      361 taagtcctaa agtacgcata attcctttat aaatatcata gaatgaagtt tcctgtttct
      421 ccctggaatg acgtaaggca tgaatatctg cttgcaccgt cttgcctaaa cggcgaatta
      481 agttgtcata aatgcgttca ttacggtttt gataggcaaa tgaacaatat tgacttaaag
      541 cctctaaact ggcatcaaac aaagtgtcac tcatcttttg cggggaactc acttgcaaat
      601 ctctttgaaa tagctcaacc aaagtactat tcattaaatt cgaaacagta gagctataat
      661 gtgaccacat cattaaataa attccagtta gtaccttttt tttgaattcg ttaatatttt
      721 ttggagttac agcagagtat cttgtgacac ccttaagata agcgattaag tcatcgtcat
      781 tctcgctatc caggctataa gcaaccaatt taatcgtatg agtatcttca atggccgcag
      841 tattgggtaa tatcactgaa aaaaaacaaa aacagcctaa ggatggtatg ctgcgtttct
      901 ttaattgatc tgttaatttc aatgcatttt gctgaaattc tgcaaactct agcatggttt
      961 ccctcccaca agggcccacc cgcaagaacc gcagcgagta atacgccttt gatgatcaag
     1021 actcttttaa tttttatatc aacctacaca taatattgca actattaatt taactatttg
     1081 ccaacaaata tttcaaatcg ctaattttat ttggatgaag aaaataaata tatttttccg
     1141 taggttattc taaactcaag agataccttt tactgtgatg atctttttgt gttggggctt
     1201 tgcaaataat aggacatgac ttttatttca cctgcttctc aatatcatta tatgttctca
     1261 agacatttca gaataaaaat gaatcgttac ataaaaagtt tatctataga atcgatcaat
     1321 tgttgatgaa ttccattaaa tcctctatta ctcattacca atacagcatc gccctctctt
     1381 actgttttga taatttcttt aactatctct ccattgtttt tgcaaatttt atacgggcaa
     1441 atccaggagc taacaaaatc ttttaaatta aaatcctgag gctccaggac ataggcacca
     1501 tcaacaggag ctaaagcatg agccatttct ccggcatgta cacccgttct catagtatat
     1561 gaagcaaact ccataacagc aaaaatacgt tgatgtcgtc cacttctttt taaagcatca
     1621 atagttttag taattgcggt tggatggtgg gcaaaatcat cataaacggt tactccatgt
     1681 cgattggaac gcacttcaag ccttcgtttc actggagaaa actgttctag agccttggct
     1741 gcaaccacag gatttactcc agcattccag gtagcagcaa ttgcagccaa accattttct
     1801 acattgaatt gccctatcaa cggccaagat acttcagcaa ccattacccc attatggagt
     1861 attttaaatt gtgaaccact atcgtcaatc agttcggcaa cccattcggc gtctccagat
     1921 agtgccagtg tttcaatacg cgaaaattgg cctcgtgata gaacttcatt cagagcctta
     1981 tcatctcgag gcttgatggc gacaccactt ttgggaattg ttcttaataa ataatgaaat
     2041 tgttgttgaa tagctgccaa gtccggataa atatcggcat gatcaaattc caaattgttt
     2101 aatactgcaa tatttggtcg ataatgcata aatttaggtc gtttatcaaa aaaagcacta
     2161 tcgtactcat ctgcctctat gacaaaccat ctccctttgc caaggcaggc gctggtattg
     2221 aaatcactag agacgccccc aatgagaaat cccggattca ttccagcttg atccaaaatc
     2281 caggcaatca ttgaagtggt agtcgttttg ccatgagttc cagctaccgc aatcacctga
     2341 tattttgata aaatattttc ggcaagccat tgagggcctg acatatatgg ttttcccgac
     2401 tccataactg cttcaagaac cggcattccc cgcttaatag cattaccgac aataaccatg
     2461 tcagattgca atgctaaagt ggaatcatcg tagccttcag tccatgaaat acctttggca
     2521 gcaagcaaat cactgatagg cggatagcaa ttggcatcgc tgcctgtcac cctatatcct
     2581 gcgtcgcggg cgagcaaagc caaggcactc ataaaggttc cacttactcc tagtatatgc
     2641 aaatgtgtca tcatgtcatc gccaaaaaga aatagttaaa atgttaacag cgactaaacc
     2701 aaaagcagct aaaactgatt ttaccggtgt agtgcctaaa gcaaatttat aaatatgggc
     2761 tgtaattaca aattgccaaa tagaaagccc caaagtaatg aacaaatata tcatacctat
     2821 aaataaaaaa atgggggttt taagattctc cgcagacaaa taagtgtcaa ttaagaacaa
     2881 tggtgtcgcc aaaacatgta taataatata ggcactcaat aaacatgttg atgtttgtac
     2941 aagcctggac gcaagatttt gaaactttaa aataacatag gtatagaata taaaagacaa
     3001 aatcaggctc gttgccatca ttaaacttaa taaccaatcg cgagaaaaat caagttcagc
     3061 gatattccat tgaatcagca ttataataga gaacagaata cacattacta caagaagaaa
     3121 aacggaataa ggagaattct ccggggtttc cttcaataga ctcagacgcc agtaataaga
     3181 aaaaagtaat tttagtgtat tcataattat tttttgatta ttttctttag gcttaagtac
     3241 tctctgaaca aggcagaata ttttatattc ctccataggc cctaagtgac ttcataactg
     3301 tttactaaat aatcttgtct tcctttagtt aaggcaattt cttcactata ttaagctgtt
     3361 gcacaatagg ctgatcccag gggccagaaa ttttataact atatcccgca attttttgca
     3421 tgctttgatt aataatttta tttgcaatcc atgcagcaag accagcaatc ggacctccag
     3481 ctatcgtagc tacaacgggt aagctggcag tgatatgcgg tgaaatgttt aaatccaaat
     3541 catataatcg ccttaccaaa tccagatccc ctttcatact agcataagct actgggccat
     3601 caatgtaact gttattggtg ctcataatgc cattattgac attaaagttg cctttaaata
     3661 tatcaaaact gtagccatca tttgagagat cactaaaatc aagttttaac cgtcttggaa
     3721 tagtttgcaa actcaatatg cttaataact tccctaaacc aagtttctct tcagtctccg
     3781 ggctcaaatg agtaatgcgg ccatttttca actgtaaata catggaacca ttcaacttgg
     3841 acagagaaaa atcatataag gaaccttgcc aacccccatg aaactctata tatcccttcc
     3901 ctgcatccac cgctggatta atcgcccaac gatcaagact cttggccaaa tctttcaaat
     3961 acattttaga ctgtagcttt gtttgattaa acttcccttt ctgaagccat tccccttcaa
     4021 aattagcttg ataataaggt gagtctatcc tgcaataatc aatcttccac tgttctgatg
     4081 tagaatgact tttaagggtg atattcccaa tttgaatttt tccaattgaa aaattatcaa
     4141 ctcttaaatt caaattgggg atttgcccag gagtagtatg tgaaggagta tctttattat
     4201 tttttgagga tccgatttca gataaatgta agtatttgac aaagccactc agagaattgg
     4261 ttgccgaatg gtaggttaaa tccgcatcga cttttttctg ttttaaattg attgaccaat
     4321 cattatcagg gcgttttttc gcatacaaag acatgttatc aaactgctga ttcaaaaagt
     4381 ttagtttacc catttttaca tcaataatat taaccaaact caagagtgag gggttcacat
     4441 tctctttaga gaatctgttt attacctctt tccatgcctg caaatcaaag ccgtctaaag
     4501 taccagctat accgaggcca ggatggtcct gatttacagc ttgcgcacta cctaaacgta
     4561 tttgacctga ttttaactta aacacgcctt tttgcttttg catcaataga tccgtactta
     4621 gtcgtccatt gtaattaact cttaaacgaa gagtcttttg tggattaaag tccagattaa
     4681 cctgtaaggg ggttttttct ttatatgttt ttcctaaagg tgctggaaga tcaactgcca
     4741 atccaatcaa atcggaactc aacttcatat tatccaaatc attaggatcg tcggtaattt
     4801 ttaataatgc cttagctaaa atattccctt tgagtacaga aaaaataggt aagtgccatt
     4861 ttttctttag ggattctaca gtgcattcac cttcaattga tattgcggtt aatggttgtg
     4921 gagatttttc cgattgaatt ttaatattca gggggtagcc gaatgccctg gcagttaagg
     4981 cactatcctg gatgcctttt tcattaaaca gtaaatttcc agtaacatcc cttacagaaa
     5041 aatctcccat ctgatgcttg atgttgatta tattattttc aaaggttaat tcacctcggg
     5101 ccagattatc atcattctct ggataaagag gtatttccaa acgcaaattg agtgatagca
     5161 tcccctttat taataatatt tttaaagcag ataatttttt ctttaatggg gaattcataa
     5221 caaaattgag catttgttgt gctttcccat gaacaattcc atgaattaat aaggtttccc
     5281 tatctttgcc aatatcatca atacgcaaat tcatttgttt aagaagagta ccttgagcat
     5341 caccatcaat gatatcaatg tcaagatttc tattttttag tttaatataa ccttccaaat
     5401 ctttaatcaa tctccattct ggagtaatta aaatatctcc gccgacaaca tggcttacta
     5461 tagaaaactc cccattgcca ttatcaaatg gaaaatcctt cgccattcca ttaatagtta
     5521 tcttgcctgt tgcttgccct aaacgtttga tgtcattatt taaccaagca aataacttgg
     5581 ctttcatgtg ttttttaggc aaataaggca tccacttctg taaatttttc ccagaaaaat
     5641 tacccactaa acgaatattg cctacagaat tttgggtcag ctgatcaata gcgccttcca
     5701 gactaatagt caattcgggt tgacttgcaa ccaagcgttc aatactcaac cgcaacccat
     5761 catttaactc tttccaatca aaagcaccat taaaaatggt cagtttttga gcgggataac
     5821 ctttgatatc gaaagttgaa ttctctgaat ctaactcaag gcgtccttcc gtaggctgcc
     5881 aatttaaaac gccggataaa ttactcactc caggaatatt atcctttgct ctccatccta
     5941 attgatcaaa tcgagttaaa atataattaa tttgattttc tttgattaat acctgaacat
     6001 cactcaatat tccatgcggt tttaactgca gtacattttg aatagccggt ggccaattaa
     6061 ttgcttcgga taatagtgat tctacaataa gggattttgt aaagatttga taggtattct
     6121 gatttttatt aaaacgaatt gacaattggt tctctggcca agtgacccct gctactctta
     6181 gtttcatatg gtctgcctgg aactgccacc cttgtttatc caatttccag cccatgttgg
     6241 cataaaatga ttggatcaac ttactatttt ttttatttaa taatttccat gccagacgct
     6301 taagtctcaa ttctgcctga attgatgaaa tattcccttt ctccaaatca acccataatg
     6361 aaacgtttcc tttacctccc tccagttgca ttgaggattt tggcaataaa ttttgccatt
     6421 gtgcaggtaa aacattaata gctgaaaaat agaagcgtcc attggtgttc ttaatttgag
     6481 aaggatcgaa ttgtacattc cctagcagtt ggaaaacggt tgcatttgtt tgttcgagtc
     6541 ttgcttcccc ctttatctta taatttcccc ccttattaac tatcgacagg tttaattcgt
     6601 taacagggat taatgacccg tcgttaaaat gtaagtgagc ggaaacatgc cgtacaatta
     6661 atttttcctg ctgagcgagc caatctaata tttctctagt tttttcaggt gtaatatcag
     6721 tgtctttcat tgcattacgt gaaattccct caatacacca atgcccgtct ttttcacgaa
     6781 aaaccaaatg catatcatca atataaagta cgccaggttg aatgcgccaa ttccataggg
     6841 atttaaataa attaacaccg acaattaacc tatccagata aattgactta ttatgaccat
     6901 catgaattct tatatgtttt aatttgacta ctggttcaaa ccagtaccac cctgtttcca
     6961 tagtttgaac agtaacaggt tgacccaaaa gtgaagtcaa atgctgttcc acctcccctt
     7021 tatattgttt agcccaggga gtaagagcgc gaaatacact ggaaaagaca gctgcaacaa
     7081 ttataaggac ggctaatacc atccaaattt ttttaaaaaa tttatttgcc aaaaggttat
     7141 tatcggtcat gttttaaact actataccat tgcttttgtg catattcatc agtttccttt
     7201 tgctcaatac gctgtaattg cttcagttca gctcttttta cgcgttcaat taacttactt
     7261 attccttctt ctgaagtttt tgcttgctta taatttaaat ccgccttcat cctgtttttt
     7321 gcaagttgac tcaagtagga gtttaattgt accaaagcag tatccaaatg attaataaaa
     7381 ttaattcggt tgcgtaaagg ccctacgtaa cttccttgtt gccccaatac ttccagttgc
     7441 tgtaaataat cttgtctgta accaactaat tgttcatgcc ttgctttatt ttgattaaat
     7501 tgctctcttg ctttcaccaa ctctatatag gcctgttgag tcgcttcctg tttaattttt
     7561 aataactgaa tcaaacgatc taatctgtca ctcattggtc attccctacc aaagttgaaa
     7621 tcagtttggt tagcgatgat aaactctcat cgtagcttac ttgttcatcc attccttgcg
     7681 ccaggtaatc tttgagttta ttcactgcta atatcgcttt atccaccagg ggatcgcttc
     7741 cttttgcata ggctcctaat aaaattaaat ccttgttttt ttcataaaca gacaggtatt
     7801 ttttaaacaa taacatatct ttcatatgtt gttcagagac aacaccctgc ataactcggc
     7861 taatagaagc ttcaagatct attgcaggat actgcccttg ctctgcaagc gctctgctta
     7921 atacaacatg accatccaaa atagctcgtg ccgcatctgc tatgggatct tgcaaatcat
     7981 ctccctcagt caagacagta taaaatgccg taattgaacc accccccgaa gtcccatttc
     8041 cagctctttc aaccaattta ggcaatttgg caaataccga tggcggatat ccttttgtag
     8101 caggagcctc accaatcgat aacgcgattt ctctttgtgc ctgggcaaaa cgggttaaag
     8161 aatcaatcag caagagaaca tgtttacctt gatcccgaaa atactcagca atgcttgttg
     8221 caaccttcgc gccgtgcaaa cgcatcaaag ggctttcatc cgctggagcg gcaactacaa
     8281 ccgctcgcct caaaccttcc agacccaaat tgcactcaat aaattctttt acttcgcgcc
     8341 cacgttctcc aattaatcca accacaacaa catcagcttc agtaaaacgc gtcatcatcc
     8401 caagcaaaac tgatttgcca actccacttc cagcgaacaa tcctatccgt tgtccgcggc
     8461 caacagttaa taagccatta atagcacgaa tcccaacatc caatggaact tcaatagggg
     8521 ctctttttaa aggattgatc acttgcccta tcaatggata agtctcaacc atttcaccag
     8581 gaggtccatc gtctataaac attcccgaac catttaatat tcttcctaac aattgatggc
     8641 caacacttac ttgagcaatg cgtccggtag gaataatttt cattccaggg gctatacctt
     8701 gtatatcacc aatagacatt aaataaacac tttcattact aaaaccaaca acttctgcct
     8761 gaacagaatg ttcatcattg attttaacca ggcaacgcgc tcctacaggc tgattaaatc
     8821 cctttgcttc tatggttaaa cctattgctc tgctgattct accacaatgt aacagtccgg
     8881 aatgatgttt attccttgtt cctgacaaaa ttttatggag ttgtgtttgg taaatggcca
     8941 tcactattcc tgagaaacgc ccattgggat tatattctct ttattaatat attcattgaa
     9001 taaggtagta aaacgagttt gaattcgacc atctaactcg ctgtgttcac cttgtaaata
     9061 aaaatctcct cgatttaatg ctggatcagc caccaataac tcatgaagac cggggatatt
     9121 actttcagaa aatacttctt taatccatcg cacatcatca ggatgcatgc ttatagactt
     9181 cttgccagtt aaagaaggta actcaccttt tattttattt aataaatcat ttagcttatc
     9241 tggattaaca gaaagctcaa cagcaacaca gtgttgactt aaccaaatga tggtttgaat
     9301 gatttcctgc gtcaattgct catctaaaat ttgaacgggc ttttgaatta aatccagcca
     9361 tttggagagc tccgctttaa ttgcattaat ttcttcttgt gcttgttgaa atccatctgc
     9421 ataacctttt tgaaaagctt cctgcttcac tacctccaat tcattaatca actcttcttc
     9481 atcagacggc gaatgttcag catctttgga agcctgatac ttccaagtgg taaattcaat
     9541 tttttttatc tctttattat acggctcaaa ttcatttgac atagttatct aaccttatcc
     9601 cgggcccaaa tcataccaaa aattaccact taggacaaat aaaatacaat tttgatacgg
     9661 actcttgctt aatgattttt actattttca taaaatgtct aaaactcatc ctgggaaaag
     9721 taactagatt aattcgtctc cgccctttgc tcccaggcta atcttgccat cttcagccag
     9781 atttcttgct atggctaaaa tatctcgctg ggcacgctcc acatcactga ctcttacagg
     9841 tccttgcacc tcgatatcat ctctgaggag atcagctgct cgcttagaca tattcgaata
     9901 gattttttct ttcgttgttt cagtagtacc cttcaatgct aattttaatt gatcagatgt
     9961 cacctctcgc aataaagttt gtacacttct atcatccatg tctttgatgt tttcaaaaac
    10021 aaacatctta tctcttatct tttcgcttaa ctcctcatcc caatccttaa ttctctccag
    10081 tacctcttct tccatagcgc catccaaaaa attaatcaca tctgctacgg ttttaattcc
    10141 gccgacagac gatgatttac ctgtagtttg tccactaagc tgcttttcaa tcacatgtcc
    10201 aagttcagaa attgcttcag gttttactgt atcaatatta cacattcgta ataacacttc
    10261 tgctcttttt tctgcgctaa agtaactcac cacttcagcg gactgctcac tgtccagatg
    10321 aattaatata gtggcaatga tttggggatg ttcattacga atcacttcag ctatcagacg
    10381 tccatccaac catttcagct tattcaaacc actgtcttta tttgatacca atattctatc
    10441 aataaatgga attgcctttt cttcacccaa tgcctcaatg agaacggttc tgaggtaatg
    10501 ctctgtatca acggttaagc tggtttgatc ttcgattgcg ctgataaagt caatcagaac
    10561 attctgcatt ttcgccttgc tgacgctatt catcgttgac atagccattc caactttttg
    10621 tacttgcctg ggctctaaat gctttaatac ttctgaagca tttttttctc ccatgcttaa
    10681 caataaaata gctgctcgct ctattccgtc cataattacc cccgatcaac ccatgatttc
    10741 actacttgtg cgacgcgttt gggctcttta tcaaccactt gacgaagcaa actcagctga
    10801 gtttcataat cctgaacatc tctgaatgat atttttccat ctaatgtaaa gtctcctgta
    10861 tcctgattat cagtggcttg atccatgtct ttattacttg ccaatgtttt taacataggt
    10921 cttaatacac caaataggat tgccaatata aaaagagccc cgccaatctg cttaatcaca
    10981 gaccaaaaac tgtctttttg ccaaaaatgt tcttctggaa ttgcttctat aggatctggc
    11041 tttacaaaac tgctgttgat cacattcaag ctatctcccc tcttaacatt caatcctatg
    11101 gcatcagaaa ccaataactt tatttgttca atttcttctt tagttagcgg tttcttctcc
    11161 agcttcttgg ttttatcatt cattacaggt cgattatcta ccaataccgc aaccgacaag
    11221 cgttttattg ttcctggctg atttttagta tgacttattg ttttgtccaa ttcgaaattt
    11281 ttagtggttt gcatgcgaac atcctttgcc tgcatttgat ctgacgatgt ttgttgaggt
    11341 aagttatttg gcgttgcatt ttgttgagga ttattttttt gcccaagaga actatttggt
    11401 tgaggagtat ttgataatgt cccaggaacc ccgcttgctt catttgacgc attacgactc
    11461 tcttgcatag tttgttcgct gcgtaaagcg gagagctccg gattgaacaa ttcttgagtt
    11521 tgttcatagc tggtaaagtc aatatctgca gatactttgg ccctgacacg gccgtatccc
    11581 aatattgggg ttaagatatc ctgaattttt tgagcgtatt gatgttccag gttttgtctg
    11641 taatcaagaa aacgctcagt ctccgaaaag aggttatatc cgctaccctc atttagcaat
    11701 tgtccgtctt gatcaactac tgttactctt cctgcgctta aattcggaat acttgatgct
    11761 actaaattga caattgctgc aatggtatgt ttctttattt caaatcctga atacacatca
    11821 ataaatacag aagcactagg ttcttgtgag tctcttacaa aggctgactc tcttggaatt
    11881 gccaagtgta ccctggccga tttaatatta ttaaatttac tgatcgtgcg tgctaattcc
    11941 gcctcaagag cttgcctata tcgggcgttt tccataaatt ggctggtatt aaacacccca
    12001 ccactgctgc ctaaaaggtc atgacctatg gcagtatcgc gaggcaatcc ttgtgcggcc
    12061 aatttcaaac gcgcgctttg cagctcatca gcaggcacca tcagcaagcc attattcttg
    12121 tcaattttga aatcaatact attgcgctct aaaatagtca aaatctctga tgagtcctgt
    12181 gcgttgagct gactatataa aggaacatag cttggatcac gagaccatag aaccactgct
    12241 acgcctattg caatactggc cgccaatcca aacaacactc caatttggcg tggtatagat
    12301 aagcttgcaa attttgtagc tgctactgaa gcattatcag ccaatgccat gatttactcc
    12361 tgtaattttt atcctttttt cagaatactt cactctgagc tttaaaactt tattgagaac
    12421 atttgcaaac tcattatggc attcaaaaac cccataaaaa gctgttattg agtttgatct
    12481 tgtttatctg ttgaaaaagt tctactaccc aaatcatcgc actaaaccgg catgttcata
    12541 atatcctggt atgcttgtac caatttgttt ctaactctta atgtcgcctc aaaccctaaa
    12601 ttggattttt gacttgctat cattacttca cccaaggaca cattaggatc gcctatttca
    12661 aagcgggtct ttaaactatc tgctgtttgt gataattcat tgacttgatt taatgcctgt
    12721 tgaaaaaccg aactgaaaga agcgtgattt aaaccagaat ccggttttat cccctcagcc
    12781 tgggcggcca gagttttcat ttgattaatt aaatttactg tattaatatc gctcattctc
    12841 tcagcccctg ttaaaccaca tacccttctt cacgcatttt tgcgagttta tagcgcaaag
    12901 tcctttcgct aacccccaat aacgcagcaa ctttttgtct gtttccttca ttttctaata
    12961 atgttttttc tattaattca tattcatgta tctgtaaatt tttactcgta ccctttgtct
    13021 tctcttccga aggcatggta gttgaattcg taatctgcaa atgctcttct tcgatgatac
    13081 cttgagtctg taacaccaga gctctttgta taacattatc cagctctctc gcattccctg
    13141 gccatgaata ggacaacatt gccttttgcg catccgggct aatgactgga actattggtt
    13201 gtttatgctg acaatgttta cggattaaat aattcgctaa tggaataata tcacttgttc
    13261 tctctcttag tgggtgccat tgcagaggaa aaacattcaa tcgatagtaa aggtcctctc
    13321 taaaacgccc tgcctgaacc tcttccttta attgcctgtt tgtcgtcgca ataatgcgca
    13381 catcaaggtt tattattttg tttgaaccaa tcctttctac ttctttttcc tgtaaaactc
    13441 taagtaattt tgcctgcaaa cctaaggaca tttcacttac ttcatctaaa agtaaagttc
    13501 cgccctgggc ttgctcaaac ttgccagggg ttgatttgta tgctccggta aaagcacctt
    13561 tttcataacc aaacagggtt gcttccagca tttgttcagg aattgcagca caattaattg
    13621 caataaaagg ttgtttgctt cgataagaat gatcatggat aaaatgtgcg agcacttcct
    13681 ttccagtacc gctttctcca ctgatcatta ctcccacatc ggattgtgcg actctcaaag
    13741 ccattgtcag taaggcttgg cttttaggat cccgtgcaat aggatcatta tcctcttcac
    13801 tcagatcagt ttttatatac tgctttattt tttcaattaa gacttgcgcg ctgaagggct
    13861 tttgcaaata atcaacagcg ccattgcgaa tcgctgtaac cgcatcctct acagaaccat
    13921 aagctgtcat tagaataacc ggcaaaccag gcctcttttt ttgtatttca tttaataact
    13981 gatttccatc taatttatcc attcttatgt cagtgagtac gacacttgga ttatgtaatt
    14041 caagaagtgc taatgcctct ttaccgtctt tagcagtgat ataggtatgg cctgagatcg
    14101 tcatagtctc agccaacgct tctcttaata ctgaatcatc ttcaactatt aaaacatgac
    14161 tcatagaaaa tcctttgttt gataaagtta ccctacgttt ctgttcaggc aaggtaaata
    14221 aatagttacc tgtgtcccta tcccttcagt cgactcaaga gtcactcgac caccatgcgc
    14281 ttttactaca gcaaaaacca ctgctaatcc aaggccggta ccttgtgctt ttgttgtata
    14341 gaacggcgaa aatgcttgtg ccctgacttc ttcagacatt ccgtttccat tatcctctac
    14401 tgatatttgt atacctgaat catccatgga tgcaagtgtt aaatcaattt gggttgcctc
    14461 agattgcagc gcattgatta tcagatttaa aattgctcca atcaaagact ctccatgtat
    14521 ggaggattca cgagcaataa gatggttatt aacatgtaat acagctgcat aagactcgat
    14581 gtatggctga gctctttgca ttaattgaga gcaccaaaac tccatatcga tatactttgg
    14641 ctctatggag gttcctctgg caaataataa taaatcctga atttgttgct ctatactggc
    14701 atgacattct tgcaatctgt gaatccagtt tttaatgcga tgatccaaac ttgggagatt
    14761 atttaaatgc tcggtataca atatggcaga agacaaagga gttctaatct gatgagctaa
    14821 ttgtgcggtc attgtaccaa tagaaaccaa tcgtttttca ttttctttgg cttcttcata
    14881 atccctgctt gcagtaatgt cagacaaagt aatcaatata ccgggtaaac tctctaaaga
    14941 cgaaatggct acgtgcacac gccttccatc agccagagac acttcatgcc catcatcagc
    15001 tcttggaaca aatgcttttt gaatcacatc tacccatagt gaaccaatca gtccataacc
    15061 aagcatcgct tcagctgaag gatttaacca caccactttg ccttgggcat tcaaaattac
    15121 caaggcggta ggcaaactgg acaaaatgtc ccattcagat aattctatct gtggtttgac
    15181 tgcacaagtt tgataatttg aataat
//
LOCUS       DATNSN010000081        15140 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.081, whole genome shotgun sequence.
ACCESSION   DATNSN010000081 DATNSN010000000
VERSION     DATNSN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.081"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            690..932
                     /locus_tag="VAH76_001916"
     CDS             690..932
                     /locus_tag="VAH76_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386527.1"
                     /translation="MTNKILTERLNNELDELGVPCLMTERVQVCSKLFNLPKFKMEAL
                     LNGVIAIDANSIQKIADELEVNKDWLLGCIKKKTKH"
     gene            1404..2296
                     /locus_tag="VAH76_001917"
                     /pseudo
     CDS             1404..2296
                     /locus_tag="VAH76_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2615..3248
                     /locus_tag="VAH76_001918"
                     /pseudo
     CDS             2615..3248
                     /locus_tag="VAH76_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
     gene            3245..4105
                     /locus_tag="VAH76_001919"
     CDS             3245..4105
                     /locus_tag="VAH76_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
                     /protein_id="HEO1386528.1"
                     /translation="MSLQLHKINSKAPLETTIEERDCQLPIPLPDTLKTVTKINSGIW
                     HTVIAGYDQYNRPQLATFGKNCDGQLGTGDCENRDTPTLAKLPPEMHTLHIIQTGFFH
                     TIIAGLDKNGCPIIASCGYNSDGQLGCGDKDNKNTLIPIKLPENMKSVEMVATGVKHT
                     VIVGKDINNKPVVATCGHNGYGQLGTGDEENRLCLTPIEIPEDITTVDYVGAGTYHTV
                     ICGRNMKNQPILALCGCNSDGELGLAPQKIAKKSSLMTNPALFFPPRQDRNAINNVMK
                     ISKFSPCNLL"
     gene            4294..4878
                     /locus_tag="VAH76_001920"
     CDS             4294..4878
                     /locus_tag="VAH76_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEO1386529.1"
                     /translation="MSTSKKILMIVGDFNEDLEVYFPYQAMLMNNYLIDTVSPGKNKG
                     DFIQTAVHDLDKPHLQSYTEKLGHLFPITADFSKITLKDYDALIIPGGRAAEYQRLSK
                     DILAIIRHFHETNKPIACICHGIQILAEAGILENKKCTTVGFCEPDVRKAGGHFIDTG
                     MDGVVVDGKLVTGATWLGNAPWMREFLHILQNEE"
     gene            complement(4948..6882)
                     /locus_tag="VAH76_001921"
     CDS             complement(4948..6882)
                     /locus_tag="VAH76_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein"
                     /protein_id="HEO1386530.1"
                     /translation="MYKIYSYLSWRIDMKTENLPQAGQEAQIDKKIHFIWVGHIMPQK
                     NIQVVSEWAEKNPGYETIIWVDKKIAPAKELDLFIQDMKSKGITVKDINEEGVCRDSI
                     RHELDQESPNYGMVSDMLRLNILAAEGGIYLDSDILCSTPFPDEIHAPFGFLLSPWSQ
                     GANNALCNDIILCSKGNQITQQLADAIEQSYIARDSFEFTHEYASMKETKGERVAKTL
                     GVTGPGFLFHQLKKMGILNDKSELSAVHWELQDQRYLINGSVEAPDYFYVPQNNKNDA
                     SWVPSIKRPGIENMSFQERLENAVQLIAFDIQKTGLFNLDHYANELKVKQNSWCIAAE
                     TPAELKPDSYLLIRPRDKTGEWALYYVDEDKKLNPVTLPVIKGAIKLSELSDPLRKFH
                     IVLSQVSDPVNPTTHELKQIGRALIDLKPKQNEWQCKNKWPDVEEIAQDLWQRVTSNE
                     TLRAQIKQCSTQFESLKPRVAELGLTRVSEAETEVEEHESTVKEQEIISQKTVVEECT
                     QEKNSAQLAPENPSEEKIKTAHDLIDEIIQDVIQLDGKLGLLGGNTRQLENGKVINIP
                     NGAAMIFDDYKKYKQGKLTAEGALESMIKIAKLSNKLNRHTFFNQRQPETGQFYKKVA
                     AIDLQTTIAAEYDNNHGLRM"
     gene            7118..8503
                     /locus_tag="VAH76_001922"
     CDS             7118..8503
                     /locus_tag="VAH76_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5624 domain-containing protein"
                     /protein_id="HEO1386531.1"
                     /translation="MKTLMTCIFSLIILFSPPFKPIVFAHTNNTNNQGLSASANNSVG
                     SNVPNNIYVTPQAFWNLYFDFTGDETPGYPKGKINISQTLFQSEMKKNSSLAQQNEGQ
                     LILFINSTLYIYNSDRQLELKQLMRTAPNSGFTEMTAISHIGPALMYLAKIKENGDAS
                     WKSQMENLLKDIQAVKVINAQTPNNWLEQVNAPAWKPHLTTIHNMIDYACSIAGNYMS
                     EVLNEKLSFDMTSLQNDFLNGNKTYPIPYNNVMIGTFMLTALQSMDQLHSKISQLKID
                     WPHAKVIIRFVAGSNVSAGVTKGSNWLVPFVQALSNNTLAADRIYITPYAAVKPSLGA
                     QELTQTDYNYYNNTVWGARHNRTIIANEVFTNITSIFLPDRPAIPGDYTYSKQPKIED
                     FLMRLKFSLAEPTEMLSNTVGFWIAGELAEKNWNYNKISIPGITTGFPQGISTYPNNN
                     PVIQYRLSQVC"
     gene            8931..10130
                     /locus_tag="VAH76_001923"
     CDS             8931..10130
                     /locus_tag="VAH76_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HEO1386532.1"
                     /translation="MLNKGYIKTTPYGLGFSELFFEDNLKKYFSDPALEVLSAEYSPS
                     ASDKMAWSVKHPKQGDHPIGVQRYKISFKKNGKIHTRHVIAKSKISDKQYLQIISNAF
                     AKCGIQTEIPIPDYLSQLEFSNLNRKEITIYHMQDSFEAFRYAMPLCYGSYLDEQEEI
                     CVLILECLDEKWLSLDPFNTSKWDQTAIEALIDAIASMHAVWYKKENAIAEIKGFKNP
                     LNTDQMQRLMPYWNALAKAVKTSNLPFLTKEDYLFHTHLIETMPQWHIKIDAMAKTLV
                     QNDCVPKNIALCNQNGVKKVLVYDWEIATIHIPQRDIVEFLSYVLPENFDSKVLGNYI
                     ERHRIKLGETTKQAIDATEWRLGFQYALYDYLIQRVFPQIPFEKLEARNIAKVYNNAR
                     RMMALLD"
     gene            10150..10887
                     /locus_tag="VAH76_001924"
     CDS             10150..10887
                     /locus_tag="VAH76_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HEO1386533.1"
                     /translation="MTHIRVILPGYNTKVLNKEMLKPFESPGLELSVDYADIAVDAVR
                     HEFDIALMAPYLIKKVIKAEQEKVHAIVIDMMADVAMRALREAVTIPVVSLAETATHV
                     AAMMGNNFAIINTTNELTPIQESLVRSYGLYDRLACIQGIQLDPHDDYRSEKTIHSLV
                     EQCADAVIKKHADTLIFGSGRLIGYREVIQKGLEEQGLHITIIEPLPTGIYFAKFLVD
                     SKISQSKYVYRPPDASTLKTYSKILGN"
     gene            complement(10927..12240)
                     /locus_tag="VAH76_001925"
     CDS             complement(10927..12240)
                     /locus_tag="VAH76_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEO1386534.1"
                     /translation="MNSIPHLILGDIITPTQTGQSLLIKKGYVLIDGDSIIEVGEQAH
                     LNQEISCTVSNYPNHLILPGLIDTHSHLPQYAISGSGDLPLMDWLNALVFPAEAAFAN
                     DLKRCQRHAELFMHACLGSGTTTINTMVTSSREATEVVCDVAARMGIRAFIGLVLMDR
                     NAPDSLLVDCDQAFNDLTFLKDNYHGKNNIHITVSPRFAVTCSAAMLRQAGEFARANK
                     LILHTHLDKDEGFDELIQSLFSTAHDYFDVFESTQCIADKTVFAHGTLLSLREVKRMG
                     DYAKQVGISHCPSSNFSFAMGMAPVSFFKDLGIEVGLGSDSGGGDSLSLFDEMRSASF
                     TNKALWRLDKKMALIDTKSWLYHATLGGAKLLGLEKQIGSIEAGKKADLIILDDRNNY
                     PLAELSSLSQELALDELQYRLARVVARSCENKLVAVYIDGKKVYQ"
     gene            complement(12422..>15140)
                     /locus_tag="VAH76_001926"
     CDS             complement(12422..>15140)
                     /locus_tag="VAH76_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO1386535.1"
                     /translation="SVPLKDTPAEKYIVGTRKIPIDVAEKLQIRSLKGPIGIPEFDKN
                     KPYNDYVVVPVYNLDDELVGLQIIQIDPAGNKATNPKNSDFFCKKYLGPTKPLREGKA
                     AVINKTDNVNVVFIAEGVETAASVASLNEVRNNYSVLASMGVSELPATLAYVKTHYPP
                     GATVVLLKDHDDPVENPFANSEFDKAKKAFVDAGYRVIVKEPPTVKEDWNDVLAKGGV
                     NELERQLNLNNPILCLPDTPARNSKVTPHFKSLYADLLKNEKLAEEQAIYGLLDKVIG
                     QLTDKLDKAQSGDDLHKTIKEIDEVVRTTEGVRTLLSETKNIPYQLRGLRRALKELKS
                     LEDSRRDSLRDNEAIDFSTDPQTNSNGDLFKAYTYVMRHCLKHLQSLKPSDIFDLKKH
                     SEILGFYEKRLNAIDNEIKRIPQLIAEFSGDDEIVGEIVIKQQKDKLKSLKLERRLYA
                     QEFHEMKQQLRLLRTAHPKGNTPYANYYEQFVENANSIMMKGKTQTKAIREELTGVWA
                     NKKQDLQEYYKKRIEAARQNFVSERKKAIPPLIEYLEGLKAIVKKRSEEIDKKALLPE
                     SRNYQMRYLQAMEELSQSEGVDKILQDWLNNLENFKTISPLIYYPPKEGETVDEVELI
                     DYDSEGEETLDELCKEIFVDKERLLETPMNVHDKGKKKREGKEGDSDPHVGVSTPVLS
                     GPSGLAEGLMQSIQPASLKTSNPLLYSTIKDFSERLAVRLYKSFEVISPTSGERQEFD
                     GLALRKKGLSIIERKTNDGTGPGLFQRNFCQQKIASKHDFVLKNVIAELKTQTSPEQF
                     IDIKEPKPLSWYSEDFTPEMKERLVNAAKSTVIKALQNLTIEFNVNKPKAYSATDYRG
                     LFFNRGLIPGGVSLRFSSLGKGNEEVAHQHMDKISEERTAKRESEIHLI"
BASE COUNT         4665 a         3065 c         2726 g         4684 t
ORIGIN      
        1 cattttgaaa aataaagggc tttgattata caaaaacaaa accaaccagt aaatatttga
       61 ccttttatta ctatattaaa accatcagat ctcaaaaaaa tcaataagat caaatcattg
      121 cacaacacat cggagtaatt ttgatagaac agatctcatg cataagctga ttattttatt
      181 tcaagacaag ctacaaacgt tttaagaatc cgagtttata tgatgtaagt gatggcaaac
      241 aaagtctaat aaaatactgg ttattttctg tgtttgagca taattaaaaa ataaatagca
      301 aataaaacca gcaatgactt caattgaacc aatgacataa cattggcaat aaaagcaaat
      361 ttgtacaata aattttctat agcaacatgt tacaaccata tcaattgtcg acgtgatatc
      421 atgaaaacga tggtctacct aacttaatac cccacagagg aatatataga ttacaaccag
      481 acgccgcgtt tgttctgtaa tatcttagaa tctatattat ctactcaagc agacatccct
      541 ttcaaatcac caaaccattc accattggat ttttcatatt tttagagcca gggatcaggc
      601 tttcaattaa ttatctgaca aaattaattt gttaatcaat ccttaagctt tagtgtttta
      661 cactgatata aaacgagaag gagaatacca tgactaacaa aatattaact gaacgcttga
      721 ataatgagct ggatgaactt ggtgttccat gtctaatgac cgagcgtgtg caagtgtgct
      781 ccaaactatt taacttgcct aaattcaaaa tggaagcctt attgaatggt gtcattgcta
      841 ttgatgccaa ctcgatacaa aaaattgccg atgaacttga ggtgaataag gattggctac
      901 tgggctgcat taagaaaaaa acaaagcact agaggttgtt gacattttct cagacagctg
      961 atctactgcg gctttgtccg cagtatccta cagacttgac tcaggatctc tgtatttata
     1021 aagccactaa tccatattta tgttgccaat ttttaaaata gcattttcct gaaatgacag
     1081 gatttaatga ttttttcgaa tgatatcttg aatctctcca ctattctggt ttttatctac
     1141 cattgtatac tcactggcat catgtgcttt cccacccgtt acttcaaaat ccaagggaaa
     1201 agcaggctaa ttgttgaagt ataaatcaac aacagtacac attgatcaaa tcttatattt
     1261 aatgcttaaa tcaatgagtt agataatctt aatttaataa aattacctct attattagat
     1321 gaagaatcaa actgattctg ttagaattgg tttatcctag atgttcaaag gaataacaat
     1381 aataaatcag tggagaacag gatatgttta gtttgaaaaa aacaactgcg gctgtgcttg
     1441 ctctaggaag cagtgctgtg tttgccggta cgatgggacc agtatgtact ccaggcaacg
     1501 taactgttcc ttgcgagaga acagcatggg atgttggtat taccgcgctc tatctgcagc
     1561 cagtttatga tgctgattgg ggctataatg gttttactga tgttggtggc tggagaaatt
     1621 ggcatgacgt tgatttagag tgggattggg gctttaaact agaaggctct tatcacttca
     1681 acacaggcaa cgacatcaat gtcaattggt accatttgga tgccgacacc gatcattggg
     1741 cgtttgttga tcaattgcac gcttacgaca ttacctggga tgctgtgaat gctgaactgg
     1801 ggcaatttgt cgattttagc gcgaacaaaa aatgcgcttc catggtggcg ttcagtatgc
     1861 ctacatcaaa acagacgtta atcgtcattt aaacggcttc tttttagata acttcaattc
     1921 caaattcaat ggatttggtc cccgtactgg ccttgatatg aattatgtat tcggtaacgg
     1981 gttaggagtt tatgcgaaat cagctgttgc tatcttggtt ggtacaagta agttcctcga
     2041 taactgcact gtctgtggat attcttatgg ttccaaaaat gcaattgtgc cagaagttga
     2101 aatgaagctg ggagcagatt atacctatgc aatggcccaa ggcgatttga ctctggacgt
     2161 tggttacatg tggtttaatt acttcaacgc actgcataat actgctgccg tgaatgttgg
     2221 attaggagct tcattggaaa ccgatttctc tgcatctggc ccctacatag gcttaaaata
     2281 cgttggcaat gtctaacaaa gattcaaaac catgttgcta atctagtcat gatcaagcag
     2341 cagtatgcaa aaaacccgta ttgacatcgg ctaatacggg tttttttatt tctctctgtt
     2401 taattaagtt agttatccta tctaagctaa cttgtaatca catgatagtt tctcaatcaa
     2461 attgagatga ttttattcga aatcaatgat tggttgccct gttggtcaac ataatcaatg
     2521 aaaaatcaca tccgtgatct gctgtgcaga ctataggaaa cgcaataagc cgctgattct
     2581 gcccgatgca taattctttt ccattccaaa gatcgtgggt tataatgaaa tcatctgtca
     2641 ccaaccgttt ataaaaataa acatgcctga tgttagcggc aaacctgatg caaaccagat
     2701 tccagatgac gtctggcttg acattcttat caattatctt caaccacagg atgctgtgaa
     2761 cctgtctgaa gtcaacaatc gcctgaaccg acttttcgca actcaacata ctcaatctga
     2821 gcaattgcac cgaaaaaaaa cattaggaat tctgaaggta tttacaggat atagttccac
     2881 cttcctgttt atacgcttca atggcaaaca aagattattt aaaacaagaa actggagtgc
     2941 agtggatagc gataacataa ccccactatt aatgcccatc tctctgccag agaacatctc
     3001 ttttattgaa tccattcaat caacatacaa acacactgtt gttgttggtc gtgataatcg
     3061 cgatcaaatt atagtggcaa caaataaaat ccataaggat tatgcacagc aagaaaccag
     3121 ccacaatcag ctgattcccc ttccattacg caataataac atttctctgg tatctgtcgg
     3181 gaataatgaa gagagttata tttttaaccc tatttttctc ttatcccgca atgcagcagg
     3241 gcaattgagt cttcaattac ataaaataaa tagcaaggct ccattggaaa ccacaattga
     3301 ggaaagagac tgccagttac ccattccatt gcctgacacg cttaagacag taacaaaaat
     3361 aaattcaggg atatggcata ccgtcattgc gggctatgat caatataacc gacctcaact
     3421 cgccaccttt ggaaaaaact gcgatggtca attaggtact ggggattgcg aaaaccgaga
     3481 tacgcccacc ctggcaaagc ttccccctga aatgcatacg cttcatataa tccaaaccgg
     3541 attttttcac accattatcg ctgggcttga taaaaatgga tgccctatca ttgcaagctg
     3601 cggttataac tccgatggcc aattgggatg cggggataaa gataataaaa acacattgat
     3661 tcccatcaaa cttccagaaa atatgaaatc agtagaaatg gtcgccactg gagtaaagca
     3721 caccgttatt gtgggaaaag acataaataa caagcctgtg gttgcaacct gtgggcataa
     3781 cggttatggg cagctgggta caggcgatga agagaaccga ctctgcttaa ctcccatcga
     3841 gattcccgag gacattacaa ctgtcgatta tgttggagca ggcacctatc atacagtcat
     3901 ttgtggacgg aacatgaaaa atcagccgat tcttgcttta tgtgggtgca actcagacgg
     3961 tgaattggga cttgctccac aaaaaatcgc caaaaaatca tccctgatga ccaacccggc
     4021 cctgttcttt ccaccgagac aagacaggaa cgcaataaac aatgtgatga aaattagtaa
     4081 attctcgcca tgtaatttgc tatgaaattt aatttgtttc catagagaaa aacgaagttc
     4141 cagaatcttg ttatttttcc ggaagaaaag aaccaatccc gctaccaaac tactctctta
     4201 cttatatctg ctctgataga caagaatttc tgggcaaagc taattaatag ctgccatact
     4261 taatgaattc aattcgtaaa aatggaatgc atcatgtcga cttcaaaaaa aattcttatg
     4321 attgtaggag actttaatga agatttggaa gtttactttc cctatcaagc aatgctcatg
     4381 aacaattacc tcatcgatac tgtttccccc ggcaaaaata aaggagattt tattcagacc
     4441 gctgtacatg atttggataa accgcatctt caatcttaca ccgaaaaatt agggcaccta
     4501 ttccccatta ctgctgattt ttccaaaatc actttaaagg attatgatgc tttaatcatc
     4561 ccgggtggaa gagccgccga atatcaacgt ttgagcaaag atattttagc tattatcagg
     4621 cattttcatg agacaaataa accgattgcc tgcatttgtc atggcataca aattctcgct
     4681 gaggccggga tacttgagaa taaaaaatgt acaaccgtcg gtttttgtga accggatgtt
     4741 cgaaaagcag gaggacattt tatcgacaca gggatggatg gtgttgtggt tgatggtaaa
     4801 ttggttacgg gagcaacatg gctaggaaat gccccctgga tgagagaatt tcttcatatc
     4861 ttgcaaaatg aggaataatc attttgcaaa attattggcc atgggcaatc agattaagtt
     4921 gaaaccaatt cactgtttat ataaaaacta cattcttaac ccatgattgt tatcgtattc
     4981 cgctgcaatg gttgtttgta aatcaattgc agccactttt ttatagaatt gcccggtttc
     5041 tggttgcctt tgattaaaaa aagtatgccg attaagttta ttagacagtt ttgcgatttt
     5101 tatcatagat tctaacgcac cttcagctgt taactttcct tgcttatatt ttttataatc
     5161 atcaaatatc atagcagcgc cattaggtat attaataacc ttgccatttt ccagctggcg
     5221 tgtattccca cccaatagac ctaattttcc atccagctga atcacatcct gaataatttc
     5281 atcaattaaa tcatgagcgg ttttaatctt ctcctcagat gggttttctg gcgctaattg
     5341 ggcagaattc ttttcttgag tgcattcctc taccacagtt ttctgcgaga taatttcttg
     5401 ttctttaaca gtagactcat gctcttcaac ttctgtttcc gcttcagaga ctcttgttaa
     5461 acctaactca gctacccttg gttttaatga ttcaaattga gtggaacatt gtttaatttg
     5521 tgctcttaac gtttcatttg aagtaactct ttgccaaaga tcctgtgcaa tctcctcaac
     5581 atcaggccat ttgtttttgc attgccattc attttgctta ggcttaaggt ctattaaggc
     5641 tcttccaatt tgctttaatt catgagttgt tggatttaca ggatcagaaa cctgacttaa
     5701 tactatatga aattttctga gaggatctga gagttctgaa agtttaatag ctcctttaat
     5761 aactggtaat gtcacaggat ttaatttttt atcctcatca acataatata gagcccattc
     5821 cccagttttg tccctgggtc taatcaacaa ataagaatcc ggttttaact cagcgggtgt
     5881 ttctgcagca atacaccagc tgttttgttt cacttttaat tcattggcat aatgatccaa
     5941 attaaataaa ccagtctttt gaatatcaaa ggctatcagt tggactgcat tttcaagtct
     6001 ttcttgaaaa ctcatgtttt ctatccccgg ccttttgata ctgggaaccc aactggcgtc
     6061 atttttattg ttctgtggta cataaaaata atctggtgcc tccacactcc cattgattaa
     6121 ataacgttga tcttgtaatt cccaatgaac cgcactaagt tcacttttgt cattcaaaat
     6181 tcccattttt tttaactgat gaaacaaaaa tcctggccca gttacgccta atgttttagc
     6241 aactctctcc ccttttgttt ccttcattga tgcatattca tgagtgaact caaaagaatc
     6301 tctcgcaata taagattgct cgatcgcatc agctaattgc tgggttattt gatttccttt
     6361 gctacataaa atgatgtcat tgcaaagagc attatttgct ccttgggacc agggagacaa
     6421 taaaaaacca aaaggcgcgt gaatttcatc agggaaaggt gtggaacata agatatctga
     6481 atccagataa atccctccct ctgcagccaa gatgtttaat cgcaacatgt cactcaccat
     6541 cccataatta ggagattcct gatcgagttc atgacgtatg ctatcccgac acacaccttc
     6601 ttcattaata tcttttaccg tgatgccttt ggatttcatg tcttgaataa aaagatcgag
     6661 ttctttggca ggcgctattt ttttatctac ccagataatc gtttcatagc cagggttctt
     6721 ttcagcccat tcggagacaa cctggatgtt tttttgaggc ataatatgac caacccatat
     6781 aaaatggatt tttttgtcaa tttgagcctc ttgaccagcc tgtggtaaat tttctgtctt
     6841 catatcaatt ctccaactta gatatgaata tattttatac agattgatat taagcccgga
     6901 tgagcatcat aagaataaaa ctcatagtac atgcctgtta agaatagcca gaacctaacg
     6961 agagcgatta agaaataaag aataactaca agccaatttc ccaagtccta acccatgagt
     7021 gtcgatggat tttcgtaacg attatcaata taattttccg gtaaatttgt ggctttaatt
     7081 tgctaggaac agagcccaaa ccaccaagga ttgaatcatg aagaccttaa tgacctgcat
     7141 tttctccctc ataatattat tttcaccgcc ttttaagcct attgtttttg ctcatactaa
     7201 taatacaaat aatcaaggtt tatcggcctc ggctaacaac agtgtgggca gcaacgtacc
     7261 taataacatt tatgtcacac ctcaggcttt ttggaattta tattttgact tcaccggaga
     7321 tgaaactcca ggatatccca aaggtaaaat taatatttcc caaaccttat ttcagtcaga
     7381 aatgaaaaaa aacagcagct tggcgcagca aaatgaaggc caattaatcc ttttcatcaa
     7441 ctcaacactg tatatttata atagtgaccg tcagcttgag ctaaaacaat tgatgcgtac
     7501 tgcaccaaat tcaggattca ctgagatgac tgcgatatca catattggcc cagctctgat
     7561 gtatctcgca aaaattaaag aaaatggcga tgcaagctgg aaatcacaaa tggaaaattt
     7621 acttaaggat atccaggcag taaaagtaat taatgcacaa acacccaata attggctgga
     7681 gcaagtcaat gcgccagcct ggaagcctca tctcactaca atccataata tgattgatta
     7741 tgcctgttct atagcaggca attacatgag tgaggtattg aacgaaaaac tatcgtttga
     7801 tatgacctct ttacaaaacg actttcttaa cggcaataaa acgtatccta tcccttataa
     7861 taacgtcatg atcggcactt ttatgttaac cgccttgcaa agcatggatc agcttcatag
     7921 taaaataagc caattaaaaa tagattggcc tcatgcgaag gtaattatcc gttttgtcgc
     7981 aggatctaat gtttcagctg gcgtgactaa aggcagcaat tggttggtcc catttgttca
     8041 agcactatcc aataatacat tagctgcaga tagaatttat atcacgcctt atgccgcagt
     8101 gaaaccatcg ttaggtgctc aagagttaac acaaactgat tacaactatt ataataatac
     8161 ggtatggggg gcacgacata accgtacaat aatcgccaac gaagtattta ctaacattac
     8221 gagcatcttc ttacctgatc gccctgctat acccggtgat tatacttatt ccaaacaacc
     8281 taaaatcgaa gattttctaa tgcgcttaaa gttttcattg gcagaaccaa ctgagatgtt
     8341 atccaatact gttgggtttt ggattgctgg tgaacttgct gaaaaaaact ggaattacaa
     8401 taaaatcagc atcccgggta ttacaactgg tttcccacaa ggcatatcca cctatccgaa
     8461 caataatcct gtaattcaat accgtttgtc tcaagtatgt tgataaacca ttttatctgg
     8521 gtgaaaaatt accagctgtt cttgccggtg gtgattgtca aattgaaccg gattatctta
     8581 aaggaattgg cataaagagt ggaattgaac gtgccaactt tctattttga cactgcttat
     8641 ctgcgcacca aaggactgga gttcctgttt gataattact accaaaagta gccatatata
     8701 agttatcatg gcaaactgat aggacagttt tatctgcaaa gaatggataa tattaatggc
     8761 tcttcacttg aacgagtaaa gaaaattctt tgctccgctt gcaagccagt tagtgaaaaa
     8821 atctaatcat ttctcgtaat cgattagaaa gactgtcttc cctaataaat gttcaataaa
     8881 gctcattgct gctattctta attgaaatag ggttggtgga gggagtgaaa atgctaaata
     8941 agggatatat taaaacaaca ccctatggat taggattttc agagcttttt ttcgaagaca
     9001 acttaaaaaa atatttttct gatcccgcat tagaagtatt gtcagcagaa tactctcctt
     9061 ctgccagtga taaaatggca tggtctgtca aacacccaaa acagggggat catcccatag
     9121 gagtacaacg ttataaaata agttttaaaa aaaacggcaa aatacacact cgtcatgtca
     9181 tcgccaaatc taaaattagt gataagcaat atctccagat tatatcgaat gcttttgcaa
     9241 aatgtggtat tcaaacggaa atacccatcc ctgattattt gtcgcaactg gaattttcaa
     9301 atttaaacag gaaagaaatc actatttacc atatgcaaga ttcgtttgaa gcctttcgat
     9361 atgctatgcc attatgctat gggtcttatt tagacgaaca agaagaaatt tgcgtattaa
     9421 ttttagaatg ccttgatgaa aaatggctat ccctcgatcc ttttaatacc tctaaatggg
     9481 atcaaacggc tattgaagca ttgattgatg caattgcctc gatgcatgcc gtctggtata
     9541 aaaaagagaa cgcgattgcc gagatcaaag gcttcaaaaa tccattaaat accgaccaaa
     9601 tgcaacgctt gatgccctat tggaatgcct tggcaaaggc ggttaaaaca tcaaacttgc
     9661 cttttcttac aaaggaagat tatttattcc atacccatct tattgaaact atgccgcaat
     9721 ggcatatcaa aattgatgct atggctaaaa cattggtaca aaatgactgt gtaccgaaaa
     9781 atattgcatt atgcaatcaa aatggggtaa agaaagtttt ggtttatgat tgggaaattg
     9841 caactattca tataccacaa cgcgatattg ttgagttttt gtcttatgtc ttacctgaaa
     9901 atttcgatag taaggtatta ggtaattata tagagagaca tcgaataaaa cttggcgaaa
     9961 caacaaaaca ggcaattgat gcaacagaat ggcgactcgg atttcaatat gctttatacg
    10021 attacttaat acagcgtgtt tttccacaaa taccttttga aaagcttgaa gctcgtaaca
    10081 tagcaaaagt gtataacaat gcccgtcgca tgatggcctt actggactaa ttttctggag
    10141 tgattaagga tgactcatat acgtgtaata ctgcccgggt acaatactaa agttttaaac
    10201 aaggaaatgt tgaaaccatt tgaaagccct ggcttggaat taagtgttga ttatgctgac
    10261 attgcggttg atgcggtaag gcacgaattt gatattgcct tgatggcgcc ttatctcata
    10321 aaaaaagtaa taaaagccga gcaagaaaag gttcatgcca tagtgattga tatgatggca
    10381 gatgtggcaa tgcgcgcact tcgcgaggcg gtgacaattc ctgtcgtcag tcttgctgaa
    10441 acagcaacac atgttgcagc catgatgggc aataattttg cgattatcaa tacaacaaat
    10501 gaactgacgc ccattcagga aagtttggtc cgtagttatg ggttatatga tcggttggca
    10561 tgtatccaag gcatacaatt ggatccgcat gacgattatc gctcagaaaa aacaatccat
    10621 agcttagttg agcaatgtgc ggatgccgtg ataaagaagc atgctgatac acttattttc
    10681 gggtcagggc gcttgattgg ttatcgtgaa gttattcaaa aaggtttaga ggaacaaggg
    10741 ttacacatca ccattattga gcccctgcct accggaattt attttgcaaa atttctggta
    10801 gactccaaaa tttcccaaag caaatatgtt tatcggccac ccgacgcttc cacgttaaaa
    10861 acgtattcaa aaattttggg gaattaatca tcttgatttt atctcgaatc tcattaggcc
    10921 tggtgattac tgataaactt tctttccatc tatataaaca gcaactaact tgttttcgca
    10981 ggatctggca accactcttg ccagacgata ttgaagttca tcaagggcta actcttgtga
    11041 caaagaagaa agctccgcca gaggatagtt attcctgtca tccagtataa tcaaatccgc
    11101 ctttttacct gcttcaatgc tgccaatttg cttctctaac cccagcaatt tcgctccccc
    11161 aagagtggca tgatatagcc agctttttgt atcaatcaga gccatctttt tatcaagcct
    11221 ccataatgct ttattagtaa aactcgctga acgcatttca tcaaaaagag aaagactgtc
    11281 ccctccacca ctgtctgagc ccaatccaac ctcaatcccc aagtctttaa aaaaagaaac
    11341 cggcgccatt cccatagcaa aagaaaaatt actggaaggg caatggctaa ttccaacttg
    11401 cttggcatag tctcccatgc gtttcacttc gcgaagtgat aacaaagtgc catgagcaaa
    11461 cacagtctta tcagcaatac attgtgtgga ttcaaacaca tcaaaataat catgagctgt
    11521 gctaaacaag ctttgaatca attcatcaaa tccttcatcc ttgtcaagat gcgtatgcaa
    11581 tatcagcttg tttgcacggg cgaactcacc tgcttgcctc aacatagctg cagaacaagt
    11641 cactgcaaac cggggagaaa cagtgatatg tatattattt ttaccatggt aattgtcttt
    11701 taaaaaggtc agatcgttaa atgcttgatc acaatcaacc agtaaactat caggtgcatt
    11761 tctatccatc aataccaatc caataaacgc tctgataccc atgcgcgcag ccacatcgca
    11821 aactacttct gttgcttctc gactgctggt taccatagta ttgatagtcg ttgtgccaga
    11881 acccaaacaa gcatgcataa aaagctccgc atggcgttga catcttttca ggtcatttgc
    11941 gaaagctgct tctgcaggaa aaaccaaagc atttaaccaa tccatcaacg gcaaatcacc
    12001 agatccacta atagcgtact ggggaagatg actatgcgta tcaattaggc ctgggaggat
    12061 taaatgatta ggataatttg acactgtaca tgatatttct tgatttaaat gagcctgctc
    12121 acccacctca ataatgctat caccatcgat caggacataa cctttcttga taagaagact
    12181 ttggccagtt tgcgtaggag tgataatatc acctaaaatg agatgaggaa tgctattcaa
    12241 ttcatatcct gagatttatt tcttactatc aaaaagtata gacaacctct tggactaact
    12301 gaaatttggg gtaaaaaata tcatagtaaa aagagctctt tcttttccac ttgggtaaga
    12361 gagagctctt ttgtaggatt aacaacttga gttattgaag caatgagttt ttatttgact
    12421 ctcatatcaa atgaatctcg ctttctcgct tagctgttct ttcttctgaa attttgtcca
    12481 tatgctgatg agctacctct tcatttcctt tgccaaggct ggaaaaacgc agcgaaacgc
    12541 cacccggaat taaaccgcga ttaaagaaca atccgcggta atctgtcgct gagtaggctt
    12601 taggtttatt gacattgaac tcaatagtca agttctgcaa tgctttaata accgttgatt
    12661 tggcggcatt aaccaatcgc tctttcattt ccggagtaaa atcctcactg taccaactca
    12721 aaggttttgg ctcttttata tcaataaatt gttcaggaga agtttgggtt tttaattccg
    12781 caataacatt tttcaagaca aaatcatgct tggaggcaat tttttgctga caaaaattgc
    12841 gttgaaaaag ccctggacct gttccatcat ttgttttacg ctcaataatg cttagtcctt
    12901 ttttacgtaa agcaagacca tcaaactcct gtctttcacc acttgttgga gaaataacct
    12961 caaaggattt gtaaagtctc actgccaatc gctcggaaaa atccttgata gtcgaataca
    13021 gcagcggatt gctggttttc aggctagcag gttgaataga ttgcatcaaa ccctcagcaa
    13081 ggccacttgg accactcaaa accggtgtac taacacccac atgcggatca gaatctcctt
    13141 cttttccttc ccgttttttc tttcctttgt catgaacatt cattggcgtt tctaataaac
    13201 gctccttatc gacaaagatt tccttgcata attcatctaa ggtttcttct ccttcggaat
    13261 catagtcaat caactctact tcatccacgg tttcaccttc tttaggagga taataaatca
    13321 atggagatat agtcttgaaa ttttcaagat tattcaacca gtcttgtaat attttatcca
    13381 ctccttcaga ttggctcaat tcttccatag cttgtaaata tcgcatttga taattacgag
    13441 attcgggaag cagggctttt ttgtctattt cctcacttct ctttttcaca attgctttta
    13501 atccttccag atattcaatc aatggtggaa ttgccttttt tctttcgctg acaaaattct
    13561 gcctggctgc ttcaattcgt tttttatagt actcttgtag atcctgcttt ttgttagccc
    13621 atacacctgt tagctcttcc ctgatagcct ttgtttgggt cttgcctttc atcatgatag
    13681 agttcgcatt ttcgacaaac tgctcatagt agtttgcata aggagtattg cctttcggat
    13741 gtgcggttct cagaagacgc agttgttgtt tcatttcatg aaattcctgg gcgtagagcc
    13801 ttctttccag tttcaaactt tttagtttat ctttctgttg cttaattacg atttcgccaa
    13861 cgatttcgtc atcaccgcta aattcagcaa ttaactgtgg aattcttttg atttcattat
    13921 caattgcgtt caaccttttc tcataaaacc ctagaatttc gctgtgtttt ttcaaatcaa
    13981 aaatatcact gggctttaaa gattgaaggt gcttaagaca atgcctcata acataagtgt
    14041 aggctttaaa taaatcacca ttactattgg tttgcggatc agtcgagaaa tcaatggcct
    14101 cattatcccg taagctatcg cgacggctat cttcaagtga tttgagttct tttagtgctc
    14161 ttcgcaaccc tcttaattga tatgggatgt tcttagtttc agataaaagt gtacgaactc
    14221 cttctgtagt tctaaccact tcatcaatct ctttaatggt cttgtgtaaa tcatcacccg
    14281 attgagcctt atccaattta tcagtcaatt gcccaattac tttatccaaa agaccataaa
    14341 tcgcttgttc ttctgccaat ttttcattct ttaaaagatc agcatataag gatttgaaat
    14401 gaggtgttac cttgctattt ctggcaggag tatccggtaa acatagaatc gggttattaa
    14461 gattgagctg gcgctccaat tcgtttactc cccctttggc taaaacgtca ttccaatcct
    14521 ccttgacagt tggaggttct ttgacaataa cgcgataacc ggcatcaacg aatgctttct
    14581 tagccttgtc aaattctgag ttagcgaaag gattctccac aggatcgtca tgatctttta
    14641 gtaaaacaac cgttgctcct ggggggtagt gagttttgac ataggccaag gtagcaggca
    14701 gttcagatac gcccattgat gctaacacag aatagttatt tctgacctca ttcaaactag
    14761 ccacacttgc cgctgtttca actccttcgg caataaaaac aacattgaca ttatctgttt
    14821 tattaataac agctgcttta ccttctcgca gaggtttagt cggtcctaaa tatttcttgc
    14881 aaaaaaaatc tgaattttta ggatttgtcg ctttgttacc agcagggtca atttgtatta
    14941 tctgcagacc aacgagttca tcatccagat tataaacagg gacaaccaca taatcattat
    15001 aaggtttgtt tttatcaaac tctggaatac caatagggcc ttttaagctt cgtatctgta
    15061 atttttcggc cacgtctatc gggatttttc gagttcctac gatatacttt tcagcagggg
    15121 tatcttttaa aggtacgctt
//
LOCUS       DATNSN010000082        14972 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.082, whole genome shotgun sequence.
ACCESSION   DATNSN010000082 DATNSN010000000
VERSION     DATNSN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.082"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..4836
                     /locus_tag="VAH76_001927"
     CDS             <1..4836
                     /locus_tag="VAH76_001927"
                     /inference="COORDINATES: protein motif:HMM:NF012321.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HEO1386536.1"
                     /translation="GPTVGTISLVADEDNLPRGNNDTASGDAAQSNLTGILPVNFGAD
                     GAGSIDFQGMHGLSAVIGNDNITYNWNASTNTLTAYRTGGTLGVNDVFKIVVNPTTGQ
                     YTFTLLAAINHHAVADNTEGLVDPFVNLNYRVIDGDGDTAIGTLKVTIDDDVPKAITP
                     EEGFVSNKAGVVKVFNLDLDSNIGNNVGADQPGSIRFINVVDGQQAQGIIDGELVNLT
                     TVNGQLIKLYISNDGMIITGSTAATEGAINSSNTVFKVTMLPDNDYNLANDKYSVEMF
                     GKIGVINTVNFSELTGESPAGNVNFKIISDQDFPGYEVLITPKDRVNPDDTVGGTVNS
                     SSTDISISNQWIDQGSGDSEILRLDYGQFAIVNNKFNIEKHFIVNGASFEIHQIQGSP
                     SNRASINVKAFDANDNNQLNDDPLDTITRIVVFTSSGGIETVIADVTSDTSFGGMTFD
                     FQGTNGVNITGLDDGYRVRAYTTDGYNRLEIDNISSTPFSIGGLGIQAIIDTEKEVKM
                     TYDVRLTDSDGDSVDSSLEINIGPSLFKVGENVADTSNSNVPHTVGEDTGVIDGSSGA
                     DVLVGDIGGIDVSQTNYNLILILDTSGSMEEQIPFGDDGSIQRIQALKNSVNELIDNL
                     AESNALNIRVHIVQFNTHASTVGTFNLIQNGTLQSTQVNNAHNGVNSLTAGGGTNYEA
                     AFIEALDWVNNNGPLTANDPLNQVLFLSDGVPTYYYAGNGTTTLGGSGSSASFDTIFQ
                     PLGDSSSGSWVDNQSEVTQLEADFGQIQAVGINLNPNTIAYNPAGTANDLTAVQILDK
                     MEGSDPSSLSNNITTAEQLTNVLAELSLLNKLNNVGSDVIAGNEENDIIFGDTLNTDK
                     LAVDSGLNLFNFPKGSGWAVFEELEANHGWSRQDTLDYIASHADELGKETVLPDGGTR
                     SGGHDTIGGGQGNDQIYGQEGNDTIDGGAGNDVIVGGTGNDTLTGGAGADQFVLVKGQ
                     GGASAGAAPIDTITDFEVNIDKIVINGNNITGVSVSTPVSNTYTITVSYSDAAATEHF
                     KVTLSNGALLNDSGKTHLNEVVISGGTATIDGTIVGAVLYLDLNANNQEDAGERLGIT
                     NQYGHVEWVVDLSKFDVNGDGQYVIGEARAVQTGGFDIDTGLSYEINLYGPVGSAVIS
                     PLTSLLQAQLEAGMDYETANAALVARLGLPEGTQIISFNPITGSGEVLAQNAGVMTAA
                     IQFAEIAAIHYSTDESHVSFAVFEAISKALLDLPEGAVADFSDKSFLQSISNHLGLDA
                     LVTSDMIDYMATSQKALEASILTLAPGEDALTAISKIQHVTQGIYAQAIEMALIGYLP
                     LDALKSMSAVLKAYADGDITDEQLNSFEDKLSAVVVNWEENNVMDTSHQASLQTDRTV
                     DDSSVKSEVNHEMAQEVNVVINDFMDEHNISLYYYAQQAQMPQEAQEQQPTEDQAEET
                     VMHDDVVQDLLLDDHSLTMLANNPQLNGGNGNDVLHGTTGNDFIRGGQGNDTMTGGGG
                     VDTFFWLSGDDDGGVDTITDFKANPVDQSSDASVLNLSDLLSDADLETNSLDNYLNVS
                     TTEEGDTTIKVDPNGNGNFDAPAQTIILEDVDLTAVFATNNSHDIVNQMIANGNLIVE
                     Q"
     gene            5015..5614
                     /gene="wrbA"
                     /locus_tag="VAH76_001928"
     CDS             5015..5614
                     /gene="wrbA"
                     /locus_tag="VAH76_001928"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEO1386537.1"
                     /translation="MSNPYILVLYYSRTGSTAQLAQYIARGVESVSGLEARLRTVPPV
                     STTCEAVDKPVPDAGAIYVSLDDLRHCHGLALGSPTRFGNMAAPMKYFWDGTTSLWLG
                     GDLVNKPACVFSSSASMHGGQETTLISMMIPLLHHGMILLGVPYTEPALTSTQSGGTP
                     YGVTHVAGGDNENPLSQDEISLAKALGERLGRVAMGLKF"
     gene            6021..6371
                     /locus_tag="VAH76_001929"
     CDS             6021..6371
                     /locus_tag="VAH76_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEO1386538.1"
                     /translation="MVPADAKLLKQAKSLRKRSTDAENCLWYYLRAHRLKGYKFKRQV
                     PIGNYIVDFVCIQRKLIVELDGGQHLLNKEYDKDRSAYFSTLSYKVLRFWNDDVLLRT
                     GDVLESIVRCLDEP"
     gene            6605..7387
                     /gene="xth"
                     /locus_tag="VAH76_001930"
     CDS             6605..7387
                     /gene="xth"
                     /locus_tag="VAH76_001930"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO1386539.1"
                     /translation="MKVISFNANGIRSSARNGFYDWLAGQDADFVCIQETKSQVEQLI
                     PEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPK
                     LSVVSLYLPSGTSGEDRQEVKYDFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
                     NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGW
                     RIDYQVITPGLTEYVADSRIFREARFSDHAPLMIEYKGDWCV"
     gene            7380..8150
                     /gene="xth"
                     /locus_tag="VAH76_001931"
     CDS             7380..8150
                     /gene="xth"
                     /locus_tag="VAH76_001931"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO1386540.1"
                     /translation="MFKLASWNVNSLKIRLEQVLQWLESTQVDVLAMQETKLTDENFP
                     AASFTDKGYQVIFSGQKTYNGVAIVSRHPLIDIQTDIPELEDPQRRILAATVKGIRLI
                     NLYVPNGGELTSDKYQYKLDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPV
                     EWEGCVLVSPAERQAFMEILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDH
                     VLLSHELNALCRQSKIDKEPRKAERPSDHAPVWVELEL"
     gene            8287..8514
                     /gene="rpmE"
                     /locus_tag="VAH76_001932"
     CDS             8287..8514
                     /gene="rpmE"
                     /locus_tag="VAH76_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEO1386541.1"
                     /translation="MKASVHPDYQTVKVTCSCGEVFETRSTLCKDLNIEVCSMCHPFY
                     TGKQKLVDTGGRVQKFRDRYNMRTGQAKSKE"
     gene            8641..9876
                     /locus_tag="VAH76_001933"
     CDS             8641..9876
                     /locus_tag="VAH76_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEO1386542.1"
                     /translation="MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGV
                     AAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRF
                     ADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVF
                     HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNML
                     LLDTKGVIHDGREDLNPYKFAFARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLM
                     APNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVR
                     ATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERV
                     PAAVAKAAIASGASQFNNL"
     gene            10085..11377
                     /locus_tag="VAH76_001934"
     CDS             10085..11377
                     /locus_tag="VAH76_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386543.1"
                     /translation="MHTENRTNYAMIGWLLCGLGAIFYSYEYLLRIAPSVMENALRTH
                     FNLSASGFGFLSSIYYLAYVPMQLPVGVLLDRYGPKRLLTMACLVCVIGTYMFTGTTI
                     FWVAATGRFLVGFGSAFAFVGVLKIATLWLPENRLAMISGMTSALGPIGAMLGDNFLE
                     LFIVKMGWVKTLNMTAAFGIVLTLVLWAGIKDKKGQHRQSGTVPTLKKGLVDLGIIIT
                     SRQIWVNGMYGCLVYLPTTVFAELWGIPYLHHAHGLTTQAAGLANSILFLGFIIGAPI
                     MGYISDRLYRRKFPMLIGASGAAIVMMMILYLPGLNESNIQALMFLLGLLYSAQAIVF
                     AVGRELSPGEAAGTAMAVTNMIVMLGAMFLQPLVGHLLDFSLSTHLSDTTGIAVDNMS
                     KLYTVDDYQFALSIIPLGILIAAILTFFLKETHAKAPK"
     gene            11358..12635
                     /locus_tag="VAH76_001935"
     CDS             11358..12635
                     /locus_tag="VAH76_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386544.1"
                     /translation="MQKHLNNMTTKKQISYGVLVCFIGAVFYCYEFILRIVPGALQTE
                     LSTALGHISATTFGQISALYYFAYSPMQMPVGMLMDRYGPRRLLTFACLCCALGSWMF
                     TLTWSIVLVGIGRFLVGFGSSFAFVGVLSLALHWLPRRYFSLVAGLITTLGMLGLVYG
                     EIKITEWSEVIGWDHVLMHIAMIGSALCILTFFVVRDGPEGYQPNKYPWPEFFHNVLK
                     VLTSHEVWLIGFVGACLYTSLSVFGELWGKTYLEQAHHLTKVEAAKTVSAVFLGWAVG
                     APLAGYISDITRRRLLPLVIGAIVSLICISFVLYCPGLSYLTLNILLFLYGVFSATEI
                     IVFIMAKECSGAKLSGTVFAVTNMIISLAGAIFQPLVGKLLDTFGDSGIVAGEHIYTV
                     DDYQVALSILPISLLLVTILAFFIKDKDHSNNS"
     gene            complement(12664..13482)
                     /locus_tag="VAH76_001936"
     CDS             complement(12664..13482)
                     /locus_tag="VAH76_001936"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEO1386545.1"
                     /translation="MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMN
                     TSYSSYLLAESNPDLVNIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRTEFNNLM
                     DSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
                     IHDDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETAS
                     KGIPVIISNHDTEFTRHHYKKAQIKSFYVPRWISCRSQMRLPVKELVAVFNCPS"
     gene            complement(13653..14849)
                     /locus_tag="VAH76_001937"
     CDS             complement(13653..14849)
                     /locus_tag="VAH76_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HEO1386546.1"
                     /translation="MINSKLIGGILLIVGTSIGGGMLALPVSTAEVGFANSLFFLFFC
                     WLVMTAGALLILEVNLRLPAGSNMISMAKSTLGLPGQVIAWITYLLLLYTLLAAYISG
                     GSDVFSSLLHKAHIDLPNWITSVIFTAMFSLIVYSGIRSVDYVNRGLMFGKLGVYILL
                     VIIISPHVSAAGLTGGSAKAFTGTLMILVTSFGFASIVPSLRDYFNDDIRSLRRVILF
                     GSLIPLFCYIVWDAVIMGVISREGENGLLSLMNSDHATSGLTEALSQSVSSQWISGFF
                     GFFTSICMVTAFLGVSLGLFDFLADGLKLKKSGNQGKYTLALTFIPPLAVVLFNPGIY
                     LHALSYAGVCCVILLLLLPAIMAWKGRQTCSQSPSETHLIPGGNPALSLIGFIAIGLL
                     IIAIMY"
BASE COUNT         4341 a         2560 c         3411 g         4660 t
ORIGIN      
        1 gggccaacag ttggtaccat aagcctggtg gctgatgagg acaatttgcc gagaggtaat
       61 aatgacacag cttcaggaga tgctgctcaa agcaatttaa ctggtatatt gcctgttaat
      121 tttggtgctg atggtgcagg tagcattgat tttcaaggta tgcatggtct gagtgctgtc
      181 ataggtaatg acaatattac ttacaactgg aatgcttcta cgaatacact gacagcctat
      241 cgaacaggag gaaccttagg ggttaacgat gtttttaaaa tagtagttaa tccgacaaca
      301 ggtcagtata cgtttactct gctggctgct attaatcatc atgctgttgc agataatacc
      361 gaaggattgg ttgatccatt tgtgaatctt aattatcgag ttattgatgg cgatggggat
      421 actgcaattg gaactctaaa agtaacaatt gatgacgatg tacctaaagc aattacacct
      481 gaagaaggtt ttgtaagcaa taaggctggt gttgttaaag tatttaattt ggatttggat
      541 agtaatattg gaaataacgt aggcgcagat cagccaggct caatacggtt cattaatgtg
      601 gttgatggtc aacaagcgca aggaattatt gatggtgaat tggttaatct tacgacagtt
      661 aacggtcagc taattaaatt atacatatca aatgatggca tgattattac gggctcaaca
      721 gcggctactg aaggggcaat caattcaagt aatacggtat ttaaggtaac tatgcttcca
      781 gacaatgatt acaatctggc aaatgacaag tacagtgttg aaatgtttgg gaaaataggt
      841 gttatcaata cagttaactt tagtgagctc acaggtgaga gtcctgctgg caatgtaaat
      901 ttcaaaatca tctctgatca agattttcca gggtacgagg ttctgattac tcctaaagat
      961 agagttaacc cagatgatac agttggtggt acagttaaca gttccagcac tgatatcagc
     1021 atcagtaatc aatggatcga tcagggtagt ggggactctg aaatattacg tcttgattat
     1081 ggtcagtttg ctattgttaa caataaattc aatattgaga agcatttcat agtcaatggg
     1141 gcaagctttg agatacatca aattcagggt tctccatcta atagagccag tatcaatgtg
     1201 aaagcttttg atgcaaacga taataaccaa ttaaatgatg atcctctgga taccattaca
     1261 agaattgttg tctttacttc aagtggtgga attgagactg tgatagcgga tgttacttcc
     1321 gatacatcat ttggtggtat gacgtttgat tttcagggta caaacggcgt gaatattaca
     1381 ggtctggatg atggatatcg tgtaagagct tatacaacgg atggatataa ccgtttggaa
     1441 attgacaata tttcaagtac cccattttct attggcggat tgggaataca ggctattatt
     1501 gatacagaga aagaagttaa aatgacctac gatgtacgtc tcacggatag tgacggtgac
     1561 agtgtggact ctagtcttga gattaatatt ggtccgtctt tattcaaagt tggggaaaat
     1621 gtagcggata cttccaattc taatgtgcct catactgtag gagaagatac cggtgttatt
     1681 gatggaagct ctggagctga tgtgttggtt ggtgatattg gaggtattga tgttagtcaa
     1741 acaaactata atttgattct tatactggat acttcaggga gtatggaaga acagatacct
     1801 tttggtgatg acggatcaat tcagcgcatt caagcattaa aaaattctgt gaatgagctt
     1861 atagataact tggctgagag caatgcacta aatattcgcg ttcatattgt tcaatttaat
     1921 acacatgctt cgacggtagg aacctttaat ctgattcaaa atggtacttt acaatcgact
     1981 caagttaata atgctcataa cggtgttaat agtttaacag caggtggtgg tactaattat
     2041 gaggcagctt tcatagaggc tttggattgg gtgaataata atgggcctct aactgccaat
     2101 gaccctttaa atcaagtact tttcttgtct gatggagtac cgacttatta ttatgctggc
     2161 aatggaacta caacattggg tggcagcggc tccagtgctt cttttgatac tatttttcaa
     2221 ccattaggcg acagtagtag tggatcatgg gttgataatc agagtgaagt aactcaattg
     2281 gaagcagatt ttgggcaaat acaagccgtt ggaattaacc ttaatcctaa tactatagca
     2341 tataatccag caggcactgc caatgatctg acggcagtcc aaattttaga taaaatggaa
     2401 ggaagtgatc cttcaagcct gagtaataat atcaccactg ctgaacaatt aactaatgtt
     2461 ttagcagagt tatcactact taataagcta aataacgtag gtagcgatgt tattgctgga
     2521 aatgaggaaa atgatattat ttttggagat acgctaaata ccgataaatt agcagtagat
     2581 tcgggactta atttgtttaa tttccctaaa ggttctggtt gggctgtatt tgaagaatta
     2641 gaagctaatc atggatggag caggcaagat accttggatt atatagcaag tcatgccgat
     2701 gaattgggta aagaaacagt acttcctgat ggtggtacgc gttctggtgg acatgacacc
     2761 attggtggtg gccagggaaa tgatcaaatt tatggtcagg aaggcaatga taccatagat
     2821 ggtggtgctg gaaacgatgt cattgtgggt ggaacaggta atgatacgct caccggtggc
     2881 gcaggggctg atcaatttgt gctcgtgaaa ggacaaggtg gtgctagtgc aggcgcagct
     2941 ccgatagaca ctattacaga ctttgaagtg aatattgata agattgttat taacggtaat
     3001 aacattactg gtgtcagtgt atctacccct gtctctaaca cttatactat aacggttagt
     3061 tattctgatg ctgctgctac ggaacacttt aaagtgacat tatccaatgg tgctttattg
     3121 aatgactctg gaaaaactca tttgaacgaa gtggttataa gcggtggcac agctaccatt
     3181 gatggaacaa tcgttggtgc cgttttgtat ttggatctga atgccaataa tcaggaagat
     3241 gcaggtgagc gacttggtat taccaatcaa tatggtcatg tagaatgggt cgttgatctg
     3301 agtaaattcg atgtcaacgg tgatggtcag tatgttatag gcgaggctcg tgctgttcaa
     3361 accggtggtt ttgatataga tacgggttta agttatgaaa ttaatcttta cgggccagta
     3421 ggttcagcag tcatatcacc actgacaagc ctcttgcaag ctcagttaga agccggtatg
     3481 gattatgaga cagctaatgc tgctttggtc gcgcgcttgg gactgcctga gggaacacaa
     3541 attatcagtt tcaatcccat tactggcagc ggtgaagtct tggctcaaaa cgcaggtgta
     3601 atgactgcgg ccattcagtt tgctgaaata gccgcaatac actattcaac cgatgaaagc
     3661 catgtttcat tcgctgtctt tgaggcaatt agcaaggcat tgcttgacct cccggaaggg
     3721 gcagtcgctg attttagtga taagagtttc ttacagtcta tttcaaatca tctgggatta
     3781 gatgctctgg ttacatctga tatgattgac tatatggcaa ccagccagaa agcgttagag
     3841 gccagtattt tgactcttgc gcccggagaa gatgctctga cggccatcag caagatacag
     3901 catgtgaccc aaggtatata tgcccaagct attgaaatgg cattgatagg gtatttgcca
     3961 ttagatgctc ttaaaagcat gagtgccgtt cttaaggctt atgctgatgg agatattacg
     4021 gatgaacaac tcaattcttt tgaagacaag ctttccgctg tggttgtgaa ttgggaagag
     4081 aataatgtta tggacacatc tcatcaggct tcattgcaaa cagatcgtac tgttgatgac
     4141 tcttcagtca aatcagaggt gaatcatgaa atggcgcaag aagttaatgt cgtgattaat
     4201 gattttatgg atgaacataa tatttctttg tactattatg ctcaacaggc tcaaatgcct
     4261 caagaagcac aagaacaaca accaacagag gatcaggctg aagagacggt catgcatgac
     4321 gacgttgttc aagatttgtt gcttgatgat catagcctta ccatgttggc caataatccc
     4381 caattaaacg gtggtaatgg caatgatgtt ctgcatggaa ctaccggaaa cgattttata
     4441 agaggcggtc aaggaaatga tacgatgaca ggtggtggcg gtgttgatac gttcttctgg
     4501 ttaagtggtg acgatgatgg tggtgtggat actattaccg atttcaaagc aaatcctgtt
     4561 gatcaatcat cagatgccag cgtccttaac ttaagtgatt tgctatctga tgcggatctg
     4621 gaaaccaata gtttggataa ttatctgaat gtttctacca cagaagaagg tgatacaacc
     4681 attaaagtag accctaatgg taatggaaac tttgacgctc cagcgcaaac aattatatta
     4741 gaagacgtgg atttaacagc tgtttttgct accaacaatt cccatgatat tgtgaatcaa
     4801 atgatcgcta atggaaactt gatagtagaa cagtaagatg aactcattac attggtgaat
     4861 atggaattca ccaatgtttt attaattgga aaatatcctc gcgacatttt tctcgctgct
     4921 atatactcat gcgttcattc gataataagc cttgcaattc atttcacgtt attaaaatga
     4981 tgaaaaaagg caaaaaacgc tatggagcag tatcatgtct aatccttata tcttggtttt
     5041 atactattct cgcactggct caacggcaca attagctcaa tatattgctc gtggggtgga
     5101 aagtgtgtct gggcttgaag ccaggttacg cactgtacct cctgtatcta ccacttgcga
     5161 agcggtggat aagccggttc ccgatgctgg cgcaatttat gtttccttgg atgatttgcg
     5221 tcattgtcat gggttggctt taggtagccc tacccgcttt ggtaatatgg ctgctccaat
     5281 gaagtatttt tgggatggaa ctacttcttt gtggctgggt ggagatctgg tgaacaaacc
     5341 ggcttgtgtg ttttcttctt cagcgagtat gcatggtgga caggagacta ccttaatctc
     5401 catgatgatt cctttgttac atcatggcat gattttattg ggtgttcctt atactgaacc
     5461 tgccctaacc agtacgcagt ctggtggtac gccttatggt gtaacccatg tggctggtgg
     5521 tgataatgag aatccattaa gccaagatga gatttcgttg gctaaagctc tgggggaaag
     5581 attggggcgt gtagcaatgg gactgaagtt ttgagatggt ttggatgttt atttcccctc
     5641 tcccttaccc tctccccaaa ggggcgaggg aatgattaat taaactatat cttctctcca
     5701 aataggcgag ggaatgatta attaaactat atcttttctc caaataggcg agggaatgat
     5761 taattaaact atatcttctc tccaaataga tgagggaatg attaattaaa actagagttt
     5821 tctaaattga tggctatgcg agttcattga ttttagagtg ataaattttt tgtttatgag
     5881 caatgatgta atttggtatt tttaaagaaa attgtagtaa ttggcgctga tatttaaaca
     5941 aaaactccct ctcccgcgaa agaaattgta taaaaaattt gggtatgatt cgcgggagag
     6001 gggtggggag aggggaataa gtggtaccag ctgatgctaa attattgaaa caagcaaaat
     6061 ctctccggaa aaggagcact gatgctgaaa attgtttatg gtactatcta agagcacatc
     6121 gattaaaagg atacaaattt aaaagacaag ttcctattgg gaattatatt gtcgattttg
     6181 tttgtattca gagaaaactc attgttgaac tagatggtgg gcaacatctt ttaaataaag
     6241 agtatgataa agaccggagc gcctatttta gcacattaag ttataaggtt ttgcggtttt
     6301 ggaatgatga cgtgttgttg agaacaggcg atgtactgga gagtattgtc agatgtttag
     6361 atgagccata gatttggaat gcgtcaattt ttctttttca cttatgagat gagggtttgg
     6421 ctgtttattt cccctctccc taaccctctc cccaaaggag agagggaata tactatttct
     6481 taagttgatg actattgttt gttcaccgat ttagttttac aaattttttt ggctagatca
     6541 ggagttttaa tttggttaaa ttaatattta actgttcagt aatgacagcg gagtatccta
     6601 agtaatgaaa gtaattagtt ttaacgccaa tggtatacgc tcttcggctc gtaatggatt
     6661 ttatgattgg cttgcagggc aggatgccga ttttgtttgt attcaggaaa cgaaatcaca
     6721 agtcgagcaa ttaattccgg aagaactcta ttttccaagg gattattttt gtgattattt
     6781 tgatgctgaa aagaaaggct acagtggggt agccatttat tcaaaatata aacctgtgcg
     6841 tattgtgaag gggctggggt ttgattattg tgataaagag gggcgttata tccaatttga
     6901 ttaccctaaa ttaagtgtgg tttcccttta tttgccttct ggaacaagtg gtgaggatcg
     6961 gcaagaagta aaatatgatt ttctggagca atttgccaga cacttgatga atttaaaaaa
     7021 tgaggggaga gaattaatta tttgtggtga ttacaatatt gcccataaaa agatagattt
     7081 aaaaaattgg cgggggaatc agaaaaattc gggattttta cccgaagagc gtgcctggat
     7141 ggatgagtta tttggttcta tggggtttgt agatgccttt agggtacaca atcaggaaga
     7201 agagcaatat acctggtggt cttatcgggc tagagcctgg gaaaaaaatg tggggtggcg
     7261 gattgattat caggtgatta cgcctggcct gacggaatat gtagcggaca gccgaatttt
     7321 tcgtgaagcg cgtttttcgg atcatgcccc tttaatgatt gaatacaagg gagactggtg
     7381 tgtttaaact ggctagctgg aatgttaatt cattaaaaat ccgtctggaa caggtgttgc
     7441 agtggctcga gtctacacaa gttgatgtgc ttgccatgca agagacaaag ttgactgatg
     7501 agaactttcc ggctgcttca ttcacagaca aaggatatca agtcattttt tcaggccaga
     7561 aaacttataa tggagtggct attgttagtc gacatcccct gatagatatc caaacggata
     7621 ttccagaact ggaagatcct cagcggcgta tcctggctgc tacggtaaaa ggaatcagat
     7681 tgattaatct ttatgtgcct aatggtggag aactgacttc agacaagtac caatataaac
     7741 tggattggtt aaagaaagta actcgcttca ttcaacatca gttaagcatt tattctaaag
     7801 tagcagttgt gggtgatttt aatattgcgc ccgaagatcg ggatgtccat gatccagttg
     7861 agtgggaggg ttgtgttttg gtcagtcctg ctgaaaggca agcatttatg gagatacttg
     7921 aattgggttt acatgacagt ttccgtaact ttgctcagga agaaaaagcg tttagctggt
     7981 gggactatag agccgcagca ttccgacgta atcgtggttt gcgcattgat catgtgctat
     8041 tgagccatga attaaacgct ttatgccgac aatccaaaat cgataaagaa ccaagaaaag
     8101 cagagaggcc ttccgatcat gcgccggttt gggttgagtt agaattataa atgtctgagg
     8161 tgtttgagaa aaaattgctt tatgagatca tttctgctat tgttttgact catttttgta
     8221 gagtatggtg cttggcaata gtgtccaggt ggcgatactt atttgaaact agaagagagt
     8281 ggtattatga aagcatcagt tcatcctgat tatcaaacag ttaaagtaac atgcagctgt
     8341 ggtgaagtat ttgaaactcg ttctactttg tgtaaagatt tgaacataga agtttgttca
     8401 atgtgccatc ctttttatac aggaaaacaa aagctggttg atactggtgg ccgtgttcag
     8461 aaattccgtg acaggtataa tatgcgtact gggcaagcca aatcaaaaga ataatatttt
     8521 aatttttgag tttttaacaa aggcacaaat tttttgtgcc ttttctctaa gagaggatta
     8581 aaaattcaaa tttttgatat gatgttttca ccgcgtgatc tgaaatagag agtatgcact
     8641 ttggataatg aagtaataaa gcagcgggca ttggattatc atgagttccc tgttccaggg
     8701 aaactctctg ttcatcttac aaaatcgact aattcccaag acgatttgtc tttggcctat
     8761 acacctggtg ttgctgctcc agttttagcc atagctgaag cgcccgaaaa tgcttatcgt
     8821 tttaccagca aaggcaatct ggttgcggtg atgaccaatg gcactgcagt gttaggtttg
     8881 ggaaatcttg ggcctttagc gagtaagcct gtaatggaag gtaaagcggt attatttaaa
     8941 cgatttgccg atcttgatgt ctttgatata gaaatcgatg cagaggatcc tcagtctttt
     9001 attgctactg caaagcgcat tgccccaact tttggcggta taaatctgga agacatcaaa
     9061 gcgccagagt gttttgaaat agaacaagca ttgattcagc agttaaatat tcctgtattt
     9121 catgatgatc aacatggtac tgcgattgta gttgcagcag gtttattaaa tgcgttggaa
     9181 ctacagaaca agaaattaag tgatattaaa attgtttgta tgggcgctgg ggctgctggt
     9241 attgcttcta tgcgacttct tgtggcatta ggagctccca aaagtaatat gctgctgcta
     9301 gataccaagg gagttattca tgatggccgc gaggatttaa acccctataa atttgctttt
     9361 gccagaaata ctctctgtcg tacacttgaa gatgctttgg tcgatgccga tgtgtttatt
     9421 ggcgttgcaa ggccagattt gctgaatgct catttattaa gtttgatggc gcccaatcca
     9481 gtgatttttg cgttatctaa ccctgatcct gaaatcaaac ctgaattggc tcatagtgtg
     9541 cgcaaggatc tggtgatggc aacaggacga agtgattatc ctaatcaagt taataatgta
     9601 ttatgttttc cttatatttt tcgtggtgca ttagatgtac gtgccacatg tattaaccag
     9661 gccatgcaaa ttgcagctgt tgaagccatt cgtcaattgg tgcatgagcc tgttcctcag
     9721 gtagtgaagg acaattaccc aggagttgtg aattgggatt ttggacctga ctatattatt
     9781 ccaaagccta tcgaccctcg tcttaaagag agggttcctg ctgctgtagc gaaagctgca
     9841 atagcgagtg gcgcgagtca atttaataat ttataaggta ataaattcac cttatagaag
     9901 taagcagttt aggtcttatt agcgtttaaa caaaggatat cttggaaaat tttttgaaag
     9961 ccatcgctgg ttttctggaa gactgcaaga tgagcttttg tgcgcttata agatatgctg
    10021 ggatgcagaa atgggaacta agataggatt taagaatact tgactaattg ttaaggagat
    10081 aattttgcat acagaaaacc gaactaacta tgcaatgata ggatggctac tttgtgggct
    10141 gggggcaata ttttatagtt atgagtattt gttacgcatt gcaccaagtg tcatggaaaa
    10201 cgctttaagg actcatttta atttatcggc gtctggtttt ggcttccttt cttccattta
    10261 ttatctagct tatgttccta tgcaattgcc agttggggtt ttattggaca ggtatgggcc
    10321 gaaaagacta ctaacaatgg cctgtttggt atgcgttatt ggaacgtata tgtttaccgg
    10381 gacaactatt ttctgggtgg ccgcaacagg tcgcttttta gttggatttg gttccgcttt
    10441 cgcctttgtc ggtgtgttaa agatcgccac tctttggtta cctgaaaacc gactggccat
    10501 gatatctggc atgacgtccg cattaggccc tatcggtgct atgctgggcg ataactttct
    10561 ggaattgttc atcgttaaaa tgggctgggt taagacactt aacatgaccg cagctttcgg
    10621 tattgtattg actttagtct tgtgggcagg gatcaaggat aagaaggggc agcataggca
    10681 aagcggaaca gttcccactc ttaagaaagg gttagttgat ctgggaatta ttattactag
    10741 ccggcagata tgggtcaatg gaatgtatgg ttgtttagtt tacctgccta ctacggtctt
    10801 tgcagaattg tggggtattc cctatttaca ccatgcccat ggtttaacta ctcaagcggc
    10861 aggattggct aattccatac tgtttttagg atttattatt ggcgctccaa taatgggata
    10921 tatttcagat cgcttgtatc gacgtaaatt tccaatgtta attggtgcta gtggtgctgc
    10981 aattgtcatg atgatgattt tatatttgcc tggattaaat gaatcaaata ttcaggcctt
    11041 aatgttttta ttaggattgc tttatagtgc tcaagccatc gtttttgcgg tgggaagaga
    11101 gttgagtcct ggtgaggcgg ctggaacagc aatggcggtg actaatatga tagttatgct
    11161 tggtgccatg tttttgcaac cgttagtagg acatttatta gacttcagct tatcaactca
    11221 tttaagtgat accactggta ttgcagtgga taacatgagt aaactatata ccgtagatga
    11281 ttatcagttt gccttatcca ttattccatt aggtatattg atagcggcca ttttaacttt
    11341 ctttctaaaa gaaactcatg caaaagcacc taaataatat gactactaaa aaacaaattt
    11401 catatggcgt cctagtttgc tttatcgggg ctgtttttta ctgttatgaa tttattctca
    11461 gaattgttcc gggagcttta caaacagagc taagtacagc tttgggacat atttctgcta
    11521 ccacctttgg acaaatttca gccctgtatt attttgcata ttcacccatg caaatgcctg
    11581 tagggatgtt aatggatcgc tatgggccga gacgactttt gacttttgcc tgtttgtgtt
    11641 gtgccttggg ctcgtggatg tttactctga cctggtcaat tgtgctggtt ggaattggtc
    11701 gattcctggt gggatttggc tcatccttcg cttttgttgg tgtcttatct ctggctttgc
    11761 attggctgcc acgtcgttat ttttctttag tggctggttt gattaccacg ctaggtatgc
    11821 taggactggt ttatggtgaa atcaaaataa ctgaatggtc agaggtcata ggctgggatc
    11881 atgttttaat gcacattgca atgattggtt ctgcgctgtg tatcttaact ttctttgttg
    11941 ttagagatgg tccagaagga tatcaaccca acaaatatcc ttggcccgag tttttccata
    12001 acgtgcttaa ggtactgacg tcccatgaag tgtggcttat tgggtttgtt ggagcctgcc
    12061 tttatacttc attatcagta tttggagagc tgtggggtaa aacctatttg gagcaagcac
    12121 atcatttaac caaagtagaa gccgcaaaaa ctgtttctgc tgtttttctt gggtgggcgg
    12181 taggagcccc cttggctggg tacatatccg atattacccg cagacgattg ttgcctttgg
    12241 tgattggtgc gatagtttca ttaatatgca ttagttttgt tttgtattgc cctggcctat
    12301 cctatcttac cttgaacata ttgctattct tgtatggtgt tttcagtgca actgaaatta
    12361 tagtttttat tatggctaag gaatgtagtg gtgccaaatt atcaggaacg gtttttgcag
    12421 taaccaatat gattatttct ttggcgggtg ctatttttca accgctcgta gggaagttac
    12481 tggatacatt tggcgacagc ggtatagtgg caggggaaca tatctatacg gttgatgatt
    12541 atcaagtggc tctttcgata ttacctattt cattactttt ggtaactatt ctggctttct
    12601 ttattaaaga caaagaccat tccaataact cttaatcttg ataaagactc ttctttgtct
    12661 ttgtcaggat ggacagttga atactgcgac tagctcttta accggtagtc gcatctgact
    12721 tctgcagctt atccagcgag gtacatagaa actctttatt tgtgcttttt tgtaatgatg
    12781 tcgcgtaaat tcagtatcat gattcgatat gatgacagga atccctttgg aggctgtttc
    12841 ctgggccaat tcagccaact caatttgatc ttctaaagaa aataattttt ggttataggg
    12901 taatgtttga ttattttcgg tcagtggcac atagggtggg tcacagtaaa tgacatcacc
    12961 gggttcagct aaggcaaaag tgtttctaaa atcgtcgtga atgaactgtg ccgaaaggca
    13021 ttttttataa aacagcatca tttccttacg tggaaaataa ggtttttcgt aaagaccaaa
    13081 agggacatta tattttcctt ttgaattgta tcggcacaaa ccattatatc catgccgatt
    13141 aagatagaga aaaaccgctg atttttgatt tgaatccatt aaattattaa attcagttcg
    13201 caattcataa tatttttctt tgcaattggc ttcgggggta aagtattgct ggcaatagtc
    13261 tataaaggat tcgccattca attgtagaat tctataaata ttaaccaaat caggattgct
    13321 ttcagccagt aggtaagatg aatagctggt gttcataaat accacgcctg aacctgcaaa
    13381 tggctctatt aaacgttggc cagtgggtaa agaagagata atttcattta aacaattgta
    13441 cttacttcct gcccatttca gaaaaggtct gatttttacc ataacttata aaatcaatat
    13501 tttaggatga agtatctctt attttcagga taaagaacag gataatgaat aaaggttcct
    13561 tatctgattt acaaaatagc ccttgtactg gcttcgcttt attttaaaat atatcaaatt
    13621 tgtacttttt aacgctgata ttcaggcttt aattaataca tgatcgcaat gataagcaaa
    13681 ccaatggcga taaatcctat aaggcttaaa gctgggtttc ctccaggaat caaatgagtt
    13741 tctgatggag attggctaca ggtttgtctt cctttccatg ccatgatagc aggtaataac
    13801 agcagtaaaa tgacacaaca aacccctgca taacttaatg catgcaggta gatacctggg
    13861 ttaaacaaca ccacggccaa tggagggata aaagtgagag ccagcgtata cttgccttga
    13921 ttaccagatt ttttaagctt taatccatca gcaagaaaat caaatagtcc taatgaaact
    13981 cccagaaatg cagtaaccat acagatcgaa gtgaaaaaac caaaaaatcc actgatccac
    14041 tggctgctta ccgattgact aagtgcttca gtgaggccgc tggtcgcatg atccgagttc
    14101 attagggaca ataaaccatt ttctccctcg cgtgagataa cgcccataat cactgcatcc
    14161 cagactatgt agcagaataa tggtataaga gagccaaaca aaataacacg acgtaagcta
    14221 cgtatatcat cgttaaaata atcacgtaag ctcggcacga tggatgcaaa tccaaaagaa
    14281 gtgaccaaga tcatgagagt accagtaaag gctttcgctg agccgcctgt taatcctgct
    14341 gcggatacat ggggactgat aatgataacc aataaaatat agacgcccag tttgccaaac
    14401 ataagtccac gattaacgta gtctacagag cgtataccac tatatacaat aaggctgaac
    14461 atggcagtaa atattacaga cgttatccaa ttcggtaaat caatgtgggc cttatgcagc
    14521 aggctgctaa atacatcgct tccacctgaa atataagctg ccaggagggt ataaagaaga
    14581 agaaggtagg ttatccaggc aattacctga cctggcaggc ccaaggttga tttcgccatt
    14641 gaaatcatat tgcttcctgc cgggagtcgt agatttacct caagaattaa taaagctcca
    14701 gcggtcatca ccaaccagca aaaaaacagg aaaaataatg agttagcaaa tcccacctcg
    14761 gctgtagaaa caggtagcgc tagcatccca ccaccaatcg aagtgcctac tatgagtaat
    14821 ataccaccaa ttaattttga attgatcacg aagtataacc caaaacagta caaatataga
    14881 acaaaatgat actttgttcc tcaaaaaaag tcaacttcct tattgtatac ctcgatcatt
    14941 ttcttttcgc aaatgacctg gcgttcaatt ta
//
LOCUS       DATNSN010000083        14947 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.083, whole genome shotgun sequence.
ACCESSION   DATNSN010000083 DATNSN010000000
VERSION     DATNSN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14947
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.083"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            205..726
                     /gene="aroK"
                     /locus_tag="VAH76_001938"
     CDS             205..726
                     /gene="aroK"
                     /locus_tag="VAH76_001938"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEO1386547.1"
                     /translation="MVKVRNIFLIGPMGAGKSTIGRALAKELKLEFYDSDEVIEERAG
                     ADISWIFDIEGEEGFRRREQKVIDELTQKTNIVLATGGGVVITPENRNALAGRGTVIY
                     LKTSLQQQFERTKRDTKRPLLQTEDLEGRLESLRDEREPFYDELADVSFETDKLTVKA
                     VANNIIKYLYGEV"
     gene            713..1822
                     /locus_tag="VAH76_001939"
     CDS             713..1822
                     /locus_tag="VAH76_001939"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO1386548.1"
                     /translation="MAKFELYAEVDVSISGHQYPIIICRNGLIDPDLINRFITSKQVL
                     IVTNRTVAPLYLGHLQSVLPSKQCDVVILEDGEEHKNQRSLFTIYDSLIQNKHHRDTS
                     IIALGGGVIGDMAGFAASTYQRGVRFIQLPTTLLAQVDASVGGKTAINHPAGKNMIGS
                     FYQPQAVIIDLNTLKTLPEREFRAGIAEMIKYALLVGGSFFERIQDVLQQGLTVHSPE
                     LPLLIAECCQVKAKIVEQDERESGLRALLNLGHTFAHALETYTDYKKWLHGEAVAIGL
                     YCAAVLSEKKGLLDNLIVDQVEKMLIHAGLPHKIPNSIDLIQLRELMSLDKKIKNNCL
                     RFIMIKKPGACYIDDSVTEDCLHNTLINVVEGEQK"
     gene            1819..3270
                     /locus_tag="VAH76_001940"
     CDS             1819..3270
                     /locus_tag="VAH76_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEO1386549.1"
                     /translation="MIDGKEPLDEGVANQPRTLFKPGAWLAKVDFINHLILFNNVLIT
                     ILSEKEGGKTCFNSLLKSHLNQQIKSVSFKAKPPFDSQDIINEFARQLHLNNKDNNIG
                     SLAAQINERKTHVLLIIDDAHHLPESFIKEVMIIIKNQEDFGFFHLCLISDYSIVATL
                     NNLAVEPFNNLIHTIELGALSETETRTYVLQRAMASHLINRPLTDTQFKQFYHLTKGN
                     LSKINSNLELFLSKCANEKQNNKKAILKRISLVASFAIVAGLSYFYFDNVYKAPDNTL
                     KLPDLTSVTTLPPINDLIKTPTQQAVPKQESVSYIASWEDSSTRQLVHYGLPKKQILD
                     DFMDEEDSLNTVAVVDKVVVIPKLKAKNISEQVGQAVVSQSKLPPIPHSSQTKLASEQ
                     PGIKKINSKLFTIQLAASHKVEDINRFKNQNKWLAQAKIRHFTNAKGSWYILTIGEYE
                     SRNQALLNIKKLPGELAKLSPWIRSVEGLSNIG"
     gene            3379..3810
                     /locus_tag="VAH76_001941"
     CDS             3379..3810
                     /locus_tag="VAH76_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO1386550.1"
                     /translation="MYKRVLFATDFDEVGVRAAEKAKKIADENGADLILVHVVEPIPA
                     YAYPGFAGFAEVEMSIREQAEKELNELAEKLGVDEKHRFIEYGSTKNEILRVAKEHKI
                     DLIVTGSHGKHGLSLLLGSTANAILHGAECDVLIVRPKISH"
     gene            3934..4920
                     /gene="ribF"
                     /locus_tag="VAH76_001942"
     CDS             3934..4920
                     /gene="ribF"
                     /locus_tag="VAH76_001942"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO1386551.1"
                     /translation="MKLLRSSTHFAIFDKGAVATIGNFDGVHLGHQHLIKALRAKADE
                     MNLPLVILLFEPQPKEYFHREKAPARLSTLREKIDVLNLCQVDYIYCIKFDARLAQTP
                     ALYFAQFYLFEALKIRYLLVGQDFRFGKNREGDVNLLKTLGANYSCEVTVQSDFLIEN
                     EKISSTRIREALQQGNLRFAAKLLGRPYSMCGRVIKGDGRGRQWGIPTANLGIHRLSI
                     PLQGVYAVQVHINSNVFYGVANIGKRPTVAGTKNILEVHLFDFHGSLYGQLVQVIFLH
                     KLRDEVKFSSVEALITQINSDIILAKTYLKQNWLDSAIDSSNDVVNGISNTV"
     gene            5033..7828
                     /gene="ileS"
                     /locus_tag="VAH76_001943"
     CDS             5033..7828
                     /gene="ileS"
                     /locus_tag="VAH76_001943"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO1386552.1"
                     /translation="MAEYKDTLNLPNTSFPMKASLSVREPEMLADWQAKGIYQKIRKA
                     RDGSKRFILHDGPPYANGHLHCGHALNKILKDIIIKSKTFSGFDAPFVPGWDCHGLPI
                     ELNVEKKVGKAGSKISPREFRAKCREYAASQIDIQRDEFQRLGVLGDWYNPYVTMDYH
                     YEANIVRALGLMIKNGHLQQGFKPVHWCIDCGSALAEAEVDYEDKTSPSIDVAFSAVN
                     PSEFLNCFETQPAVKPLILPIWTTTPWTLPANEAVCLHPEIDYALIDAGNSYYIVATD
                     LVESVMARYGISHYKTSGSAKGRVFEHFKLQHPFYKRQVPVVLAEHVTTESGTGCVHT
                     APAHGPDDYLVGQFYQLPLINPVMANGCFAENVELFAGISVLKANETILAVLSERNVL
                     LASESIRHSYPHCWRHKSPMIFLATPQWFISMDKSNLRQAIINEIDKVNWVPDWGKAR
                     ISNMVENRPDWCISRQRSWGTPMPLFVHKTTRELHPDTLELIERVAMMIEKSGIDAWF
                     DLDSSELLGDDAKHYDKITDTMDVWLDSGISHYSVLKHNNDLDFPADVYFEGSDQHRG
                     WFNSSLTTAVAMYGVAPYKTVLTHGYTVDAEGKKLSKSKGNYVALDKLVNQHGADILR
                     LWVASTDYRHEVSISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIEAKELLEL
                     DRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDS
                     IARRSCQTAMYHMVKAFTIWLAPILSFTAEEIWQTIPGNNSESIFIEHWYNAWPTIDA
                     VNMEDWEQLHIVRDEVNKALEETRQRGEIGSALAAEVTVYADAKVLPKLTRLGEELRF
                     LFITSEAKACPISQSPKGLAVTDCGVSIQVTASVHEKCARCWHRREDVGQNQEHPELC
                     LRCVGNISGYHEERLYI"
     gene            7825..8289
                     /locus_tag="VAH76_001944"
     CDS             7825..8289
                     /locus_tag="VAH76_001944"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEO1386553.1"
                     /translation="MKKWPWLVLSFLVIICDQLSKYWVGVLLTPYKPMPVMPMLNFTL
                     AFNTGAAFSFLSGAGDWHRWFFAGFSFLMSIILLIWLVRTPEQARFQSVGISLILGGA
                     IGNLIDRGLHGYVIDFIDVYYKHHHFATFNLADSAICIGAAILVLDLLIRRE"
     gene            complement(8586..9149)
                     /locus_tag="VAH76_001945"
     CDS             complement(8586..9149)
                     /locus_tag="VAH76_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEO1386554.1"
                     /translation="MIIEKILGNQSVIISLDIDNFLFDRLDQIANSDLDLVEINSSDT
                     NLLVKIKTQYPSLKIGASGIIDTQQLEQCYQAGVHFASSPGFLASIAQTASVYSMCYF
                     PSVATISEAMAAANIGFKQVRPYPANLTFCNALSKYLPGLNLFPAEIDWNEAELFLNL
                     PAVAAVIIHNPDHKQLNGLARLAQSII"
     gene            complement(9325..11514)
                     /locus_tag="VAH76_001946"
     CDS             complement(9325..11514)
                     /locus_tag="VAH76_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LidA"
                     /protein_id="HEO1386555.1"
                     /translation="MAKDNKSHQVKTSEGSLESVKTKEKEAVVEKMRVEDSKKEDKLS
                     MPTTKKESQPNEPAKPFKTSFEKWIESSLLDPQAKEDKGSTINLGREGLKNASQVKKF
                     LLSPAGKDVIAELGAQMALQRNINLQNQQDRMEHELFKRRLMAALFLWYLSKKSHAAE
                     KVKEIIREYNEKAIKNAEKASKPSQQSTPSTSQADKEIQKMLDEYEQAIKRAQENIKK
                     GEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMKAFSKDS
                     EKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSL
                     KDAHLAINKDQEVVEHKGQFYLLQKGQWDAIKNDPAALEKAQKDYSQSKHDLATIKME
                     ALIHKLSLEMEKQLETINDLIMSTDPKENEEATKLLHKHNGLNLKLANLQDMLAVHRK
                     EKSFFNEKGEKVTSLNDAHYVIGKDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGE
                     DWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERIEETKQQLED
                     NGKEKIEIANNISKLQSTVGTALNELNQSNINQESVTLTPLGGGGTTLSPKPGPSLAA
                     VTTFFRTKIQEMKEMNSPGMTRDELRQFQSQIPEGAARNYFISALTQMPRTGPVPFQI
                     MQAMLRNLERFGVDTTKPGVTSIRSKTDEVVEQRFNPTPSLSPFKTS"
     gene            complement(11756..14881)
                     /locus_tag="VAH76_001947"
     CDS             complement(11756..14881)
                     /locus_tag="VAH76_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386556.1"
                     /translation="MPDFETTDGQTQVIIPLRVTPHTHKAMPTITGSIFSHEAGTVFY
                     GNYNMEYLLPGGDRLRLIANHNKKTVQLMLFTHNPELLKILPGDRPSSLVADVINHVN
                     RYKVQAESYKKPLSHEQEEKINLANACIRSLNHLLKKERQLKPHDHAGQEELRRKIIS
                     IIEHCQNDNRMIANNPVVSEGSFGYMLYDAHKTAQHYRFNRVFSVSRQDQMDFTKVTK
                     TQLGKPITPCFVWDSEIHIGHSSRELDDALRVICQQYQLCPAPALNNIPANRFAKLER
                     FISKLWHDGHDWINYLAVTTKPSHKTEIQKRSDGVSITKITPYYKLKGLPQIGYNSLA
                     AMVNHLVENSAQQTEVNSLKEAKRLLSHLPNGNWVIVPQKNTVLLRLENKLIHLKYFI
                     HDQLFYPLPDGEDLYILSQISKRHLYLPERISLRFKAFISRIPDFFKGFYKSMSQFII
                     HDLHEEFFNHVHATHAPKQHLSERDQRPKSKKIKRTSLHEALQNQGLLANGQTLEEFI
                     SDQINNSPYVIARANHPPTVHTYENPLHRALGVVRHLAGFFIDTSERNPMIGTLAMAA
                     YVYGAGAVLAPNVLTDILKKLHLSGLIYGIEPVQKLARLMNHGKISEAISASATLWQG
                     MVVSGNLDKFFVDAVSVLKEDPAEIAIIVSLALSLGYGLTKAIPSLQHEMGDFPYTNY
                     AALGGKGGAAIYDTIMHPGDDWFLGTCKWFCKNIINIGKLLVAPFFEGFYYGFFDGFI
                     NGWKKSGKLIKQLGKQTLAASFDLILAILTIPLLEASSLLIHVPFRGITNFFRKLLSV
                     LGNFSAIGKLLIDIAERPSTNNFISEFTLSPLYGFTSPFGHFSDNPVTNIGINVIRLL
                     FLPLLQLIKNILILPLIDILSLTIRVNLSIINPISRLLAYSTGTILYHFGQIWDHSAG
                     VLFSTSAYGVTILCNHIDSKAGELKQYLLSLIEINRGKVYQWAFHDEDILTHKIVQDN
                     QYYFNDPRRCELIPHSDSHCLLRNLLLDEKSSTNKVKDNQKSQQLHFPGLFHGATQTS
                     DTMPQEHTLTI"
BASE COUNT         4408 a         2561 c         3136 g         4842 t
ORIGIN      
        1 gtatttttag atagtaattg gatgtattcc taatttgtgc tatttacttt aaatgcttca
       61 aatataaggt cttgtgttat tctatcgagg ctgttggagt agtaaatatc atgaaatcaa
      121 ctaagacgtg tatctaattt gttgttataa tagcacggct aattatgaca attcagtaga
      181 agaggtaagt aaaaaaaaat gagcatagtt aaagtacgaa atatttttct cattggtccg
      241 atgggtgctg gtaaaagtac tataggacgt gctttggcaa aggagctcaa actggaattc
      301 tatgactcag atgaagtaat tgaagagcgt gcgggcgcag atatatcgtg gatctttgat
      361 atagaaggtg aggagggatt tagacgtcga gagcaaaagg tcattgacga attgactcag
      421 aaaaccaata tagtattggc aacgggtggc ggagtcgtca ttactcctga aaacaggaat
      481 gccctggcag gccgtggcac agtgatttat ctcaaaacat ctttgcaaca gcaatttgaa
      541 agaacaaagc gtgataccaa acgtccttta ttacaaacag aagatcttga gggacgcctg
      601 gagtcactca gagatgagcg agaacctttt tatgacgaat tggcagatgt cagttttgaa
      661 acagataaac tgactgtaaa agctgttgca aataatataa taaaatactt atatggcgaa
      721 gtttgagctt tatgccgagg ttgatgtttc gatttcaggg catcaatatc caataattat
      781 ttgccgcaat ggtttaattg atcctgattt aattaatcgt tttatcactt caaaacaggt
      841 gcttattgtt actaatcgaa cagtggcacc tttgtatcta ggtcatttgc aatctgtttt
      901 accatcaaaa caatgcgatg ttgttattct ggaagacggt gaagagcata aaaatcagcg
      961 cagtttattt actatttatg attctttgat tcaaaacaaa catcatagag atacctcaat
     1021 catcgctttg ggcgggggag tgattggtga tatggctggt tttgcagctt ctacgtacca
     1081 acgaggtgtg cgatttattc agctgcctac tacgttactg gctcaggttg atgcttcagt
     1141 tggtggtaaa acagcgatta atcatcctgc cggcaaaaat atgattggca gtttttacca
     1201 acctcaagcg gtcataattg atttaaatac cttaaaaaca ttacctgagc gtgagtttcg
     1261 tgcaggtatt gccgagatga taaaatacgc tttgttggtc ggtggctctt tttttgaacg
     1321 aattcaagac gttctgcaac aaggattaac ggtacatagt ccggaattac cactattgat
     1381 agcagagtgt tgtcaagtta aagcgaaaat tgtagagcaa gatgaaaggg aatccgggtt
     1441 gcgtgctttg cttaatctgg ggcatacttt tgctcatgcg ctggaaacgt ataccgatta
     1501 taaaaagtgg ttacatggag aagccgttgc cataggttta tattgtgcgg ctgtattatc
     1561 agagaagaag ggcttgctgg ataatctaat tgtagaccag gtcgaaaaga tgctcataca
     1621 tgctggtttg cctcataaaa ttccaaattc catagattta attcaattaa gagagttgat
     1681 gagtttggat aagaaaatta aaaataattg tttacgtttc ataatgatta aaaaaccagg
     1741 tgcttgttat attgatgatt cagttactga agattgtttg cacaatacac tgattaatgt
     1801 ggtagaagga gaacaaaaat gatagatgga aaggagcctc ttgatgaggg agttgccaat
     1861 cagcccagaa ctctattcaa accaggagcc tggcttgcaa aagttgattt cattaatcat
     1921 ttaattctct ttaataatgt actaataacc attttgtccg agaaagaggg cggaaaaact
     1981 tgttttaact ctttgttgaa aagccatttg aaccaacaaa ttaaatcagt atcatttaag
     2041 gcaaaaccgc catttgacag tcaagatatt atcaatgagt ttgccaggca gttgcatctt
     2101 aataataagg ataacaatat tggttctctt gcagcacaaa ttaatgaaag aaaaacgcat
     2161 gttttgctga ttattgatga tgcacatcac ttgcctgaaa gttttatcaa ggaagtgatg
     2221 attattataa aaaatcagga agattttgga tttttccatc tttgtttaat ttctgattat
     2281 tctatcgttg ccacattgaa taatcttgct gttgagccgt ttaataattt aatacacaca
     2341 atagaactcg gggcattaag cgagactgaa accagaactt atgtattaca acgagcaatg
     2401 gcttctcatt taatcaacag gccacttact gacacgcaat ttaaacagtt ctatcactta
     2461 accaaaggga acttatcaaa aatcaattca aacctggaat tatttttatc caagtgcgct
     2521 aatgaaaagc aaaataataa aaaggcaatt ttaaaaagaa taagtttagt cgcgagcttc
     2581 gctattgttg caggattgtc ctatttttat tttgataatg tctataaagc acctgataat
     2641 actctcaaac tgccagattt aacttctgta acaacacttc cacccataaa tgaccttata
     2701 aaaactccca cacaacaagc tgtccctaaa caagaatctg taagctatat tgcttcatgg
     2761 gaagattctt caacgaggca gctagtgcat tatgggttac ccaaaaaaca aattttagat
     2821 gattttatgg atgaagagga tagtcttaat acagtagctg ttgtagataa ggtcgttgtc
     2881 attccaaaac tgaaagctaa aaacatttca gaacaggttg ggcaagcggt agttagtcag
     2941 tcaaaattac ctcctattcc ccattcatca caaacaaaat tagccagtga gcaaccaggc
     3001 atcaagaaaa ttaatagtaa gctgtttact atacagttgg ctgccagtca taaagtagag
     3061 gatatcaaca ggtttaagaa ccaaaacaaa tggttagctc aggcaaaaat aagacatttt
     3121 actaatgcga aaggtagttg gtacatatta actatcggtg aatatgagag caggaatcaa
     3181 gctctgctga atataaaaaa actaccaggc gaattagcca aactaagtcc atggatacga
     3241 tctgtggagg gactttcaaa cattggttag actaagataa tcagcttgga tatagctgtt
     3301 agttgttgat tgattctggg aaaataggct taaatctgct aatcttaact tagctatcag
     3361 aattttaagg agaaaaagat gtataaaagg gtattgtttg ctactgactt tgatgaggtt
     3421 ggggtgcgag cagctgagaa agctaaaaaa attgctgacg agaatggtgc tgatttaatt
     3481 ttagttcatg ttgtagagcc tattccagct tatgcttacc caggttttgc tggatttgca
     3541 gaagtagaaa tgtctattcg tgagcaagct gaaaaagagc tgaatgaatt ggcagaaaaa
     3601 ctgggtgtag atgaaaagca tcgttttata gagtatggtt caactaaaaa tgaaatccta
     3661 agggtagcaa aagaacataa aattgattta attgtgacag gaagtcatgg aaaacatggc
     3721 ctttctttat tattaggttc aaccgctaat gcaatcttgc acggtgctga atgcgacgtc
     3781 ttgattgtaa gacccaaaat aagccactaa gagcgccctc taacttaaga aattccgtac
     3841 tacctggtag tacggataat gttttataga tttctcaata tctcagaaaa ccagtatact
     3901 atgtgttttt tattcttctg attaaaacga ttcatgaaat tgttacgcag ttctactcat
     3961 tttgctatat ttgataaggg tgcagttgcc actattggca attttgatgg cgtgcattta
     4021 ggccatcagc atttgattaa ggccctaaga gcaaaggctg atgaaatgaa cttgccttta
     4081 gttattttat tgtttgagcc tcagcctaaa gagtattttc acagagagaa agcaccagca
     4141 cgactttcta ccttgagaga aaaaatagat gtcttaaact tatgtcaagt ggattacatc
     4201 tattgcatca agtttgatgc aaggctggct caaactcccg ctttgtattt tgctcagttt
     4261 tatttatttg aagcattgaa aatacgctac cttctggttg gccaggattt tcgttttggt
     4321 aaaaacagag aaggtgatgt taatttactc aaaacactag gtgctaatta ttcctgtgaa
     4381 gtgacagtgc aatctgattt tcttatagaa aatgaaaaaa tcagttcgac gagaatcaga
     4441 gaagcgttgc aacaggggaa tttgcgtttc gcagccaagt tgttgggaag accatacagc
     4501 atgtgtggac gtgttatcaa aggagatggt cgtggtcgcc aatgggggat accgacagcc
     4561 aatttaggca ttcatcgttt atcgatccct ttgcaaggtg tatatgctgt acaagtccat
     4621 attaattcga atgtctttta tggggttgca aatattggaa aacggccaac agtagctgga
     4681 actaaaaata ttttggaggt ccatttattt gattttcacg ggtctctata cggtcagtta
     4741 gtgcaggtaa ttttcttgca caaacttcga gatgaagtta aattctcaag tgtggaagcg
     4801 ttaatcacgc agattaatag tgacatcata ttggcaaaga cctatttaaa acagaattgg
     4861 cttgatagtg cgattgattc atcgaatgat gttgtgaatg gaatcagtaa taccgtttaa
     4921 tttaccctta attggggtat tttttgtaag ctccctttgg gctgtggaga taaaacagaa
     4981 caagggcatt taaaattatc aaaaacttaa tgatttggca gagtaggcac ctatggcaga
     5041 atataaagat actttaaacc ttccaaacac ttcatttccg atgaaagcaa gtttgtcagt
     5101 gcgagaacct gaaatgttgg cagattggca agcaaaggga atttatcaga aaattcgtaa
     5161 agctcgtgat ggaagtaagc gctttatttt gcatgatggt ccgccctatg ccaacggaca
     5221 tctccactgt ggacatgcgc tcaataaaat tctcaaagat attattatta aatcaaaaac
     5281 ctttagtggc tttgatgccc cctttgtccc cggttgggat tgtcatggct tacctataga
     5341 attgaatgta gaaaaaaaag taggtaaagc agggagcaag attagtccaa gagaatttcg
     5401 tgccaaatgt cgagaatacg cagcaagcca aattgatata caaagagacg agtttcaacg
     5461 attgggagtc ttgggagact ggtataaccc ttatgtgact atggattatc attatgaagc
     5521 caatatagta cgagctttag gtttaatgat taaaaatggt catttacaac aaggctttaa
     5581 acccgttcat tggtgtatag attgcggatc tgcgttggct gaagcagagg tggattatga
     5641 ggataaaact tctccatcca ttgatgtggc gttctcagca gtaaatccat ccgagttcct
     5701 taattgtttt gaaactcagc cagcggtgaa accattaatt ctgcctattt ggactacaac
     5761 gccatggaca ttgcctgcta acgaagcggt atgtttgcat cctgaaattg attatgcatt
     5821 gattgatgca ggtaattctt attatatcgt ggctactgat ttggttgagt cggttatggc
     5881 gcgttatgga atcagccatt acaaaacatc gggctccgct aaggggcgag tctttgagca
     5941 ttttaaactg cagcacccat tttataagcg tcaggttccc gtagttcttg ctgagcatgt
     6001 gacaacagag tctggtacgg gttgtgtgca tactgcacct gctcatggcc ctgatgacta
     6061 tttggtaggt cagttctatc agttgccatt aataaatcct gtcatggcca atggatgttt
     6121 tgctgagaat gtagagttat ttgcaggcat ttctgtactg aaagcgaatg agactattct
     6181 agcagttttg tcggagcgga atgttttgtt agctagtgaa tctatcaggc acagttatcc
     6241 gcattgttgg cgtcataaat cgccaatgat atttctggct acgccccagt ggtttatctc
     6301 aatggataaa tccaatttgc gacaagcaat aattaacgag atagataagg ttaactgggt
     6361 tcctgactgg ggtaaggcgc gcatcagtaa tatggttgaa aataggcctg actggtgtat
     6421 atcaaggcag agatcttggg gaacacccat gccattgttt gtgcataaaa ccaccagaga
     6481 attacatcct gacactttgg agttaataga gagggttgct atgatgattg agaaatcagg
     6541 catagacgcc tggtttgatc tggatagctc cgagttgctt ggcgatgatg ccaaacacta
     6601 tgataaaatc acagatacca tggacgtttg gctagattcc ggaatatcac attatagcgt
     6661 tttaaaacat aataatgatt tagattttcc ggctgatgtc tattttgaag gatccgacca
     6721 gcacagaggt tggtttaatt cctcattaac aactgcagtt gcaatgtatg gggttgctcc
     6781 ctacaaaacc gtattgactc atgggtatac tgttgatgcc gagggaaaaa aactatcaaa
     6841 gtctaaagga aattatgttg ctttagataa attagtgaat cagcatggtg ccgatatttt
     6901 aagattatgg gtagcgtcaa ctgattatcg tcatgaagta agtatttctg aggaaatcat
     6961 taagcgtaac gcggatgctt atcgaagaat tcgtaataca gcgcgatttt tattagctaa
     7021 tttatttgac tttaatccag ccagtgattg tattgaggcc aaggagttat tagaactgga
     7081 caggtgggct ctaaagcgtt gccaactttt acaagaagaa ataattactg cctatgaaaa
     7141 ttaccacttc catttgattt accagaaaat ccataacttc tgcgcagtgg atatgggtag
     7201 tttttatttg gatttgataa aggacaggca atacaccacg gcaaaagaca gtatagcgag
     7261 gcgatcttgc caaacagcga tgtatcatat ggtcaaagca ttcactatct ggttggcgcc
     7321 tatcttatct tttacagctg aggaaatatg gcaaacaata cctgggaata atagcgaatc
     7381 tatttttatt gagcattggt ataatgcctg gccaactatt gacgcagtga acatggagga
     7441 ttgggaacag ctgcacatcg ttcgtgatga agtaaacaaa gctttagaag aaaccaggca
     7501 aagaggtgaa attggttctg cattggcggc tgaggtaact gtttatgctg atgccaaggt
     7561 attaccaaaa ttaacgcgtt taggcgaaga gttacgcttt ttattcatta catcagaagc
     7621 gaaagcttgc ccaattagtc aatcccccaa aggattagct gttactgatt gtggggtatc
     7681 aatacaggtt acagccagcg ttcacgaaaa atgtgctcga tgttggcata gacgggaaga
     7741 tgttggtcaa aatcaagagc atcctgaact gtgcttgcgc tgtgtaggaa atatcagcgg
     7801 atatcatgaa gagaggcttt acatatgaaa aaatggcctt ggcttgtact tagtttttta
     7861 gtaattattt gcgatcagtt aagtaagtat tgggttgggg tgttattaac tccatataaa
     7921 cccatgccag ttatgcctat gttgaatttc actttagctt ttaacacagg cgctgctttt
     7981 agttttttaa gcggcgcagg cgattggcat aggtggtttt ttgcagggtt tagtttttta
     8041 atgagtatta ttttgctgat ctggttagta cgtactccag aacaggcacg atttcaatct
     8101 gttgggataa gtcttattct aggaggcgct attggcaact tgattgacag aggtcttcat
     8161 ggttatgtga ttgattttat tgacgtttat tataaacacc accattttgc cacgttcaac
     8221 ttggccgata gcgctatatg tataggagcc gcaattttag ttctggattt attgattcgt
     8281 agggaataga ctcaaactgc ctagaaaata atgtcataag ttagtgtcct catgtttgac
     8341 tatcctctaa cagtagggtt tgcgttctgg ttagatgcaa ataatactga aaaagcacag
     8401 aatagatggc tgtttttgtt caagatgggc gaaaaaagac tttctcggca ggaggctgat
     8461 ctcttacgga aataatagct ccgaagtggt ttagtatctt aacccttttc aaagtcaaaa
     8521 tactatacca aagcttgtca tgcattaatg gaaaacaatg cttaccaatt agaatcgatt
     8581 tattttcaga taattgattg tgctaacctg gctaatccat tcagttgctt atggtcaggg
     8641 ttatgaataa ttactgctgc tacagctggc aagtttagga aaagctcagc ttcattccag
     8701 tcaatttcag ctgggaacag atttaatcct ggtaaatatt tgcttagagc attacaaaat
     8761 gtcagattgg cggggtaggg tcgcacttgt ttaaatccaa tattcgctgc ggccatagct
     8821 tctgatatgg tagctacact tggaaaatag cacatcgaat aaacacttgc agtttgggct
     8881 atagaagcta aaaagccggg gctagaggca aaatgtacac ccgcctggta gcattgctct
     8941 aattgttgag tatcaattat gccgctggct ccaattttta gagatgggta ttgggtttta
     9001 atctttacca ataaattggt atcggacgag ttgatctcta ctaaatccaa atcggaattt
     9061 gcaatttgat cgagtctgtc aaataaaaaa ttatcgatat ccaatgatat gatcacagat
     9121 tgattaccta agattttttc aataatcatg ttttattccg aaaattctgc aataatcttt
     9181 caatgttaga agacttgctg attgttggca agggtattga taagaaaaac aacaatttgt
     9241 ttgatgatga ggtgggggta gagcaccctg aattatgatt ctcagacaat aatccagggt
     9301 agggagatga aatataattc taccttatga tgtcttgaat ggagataaag acggagtggg
     9361 attaaatcgt tgttcaacaa cctcatctgt tttgcttctt atagaagtca caccgggttt
     9421 tgtagtgtct accccaaatc tctccaggtt gcgcaacata gcttgcatta tttggaacgg
     9481 aactgggcca gtgcgtggca tctgagtgag tgcgcttata aaatagttgc gtgctgcccc
     9541 ttcaggaatt tgactttgga attgtcgcag ctcatcacga gtcatccctg ggctattcat
     9601 ttccttcatt tcttgtattt tggttctgaa gaaggtagtg actgcagcaa gacttggtcc
     9661 tggttttggt gaaagagtcg tgccacctcc tcccaatgga gtcaaagtta ctgactcttg
     9721 attgatgtta gattggttta gttcatttaa agcagtacca actgtagatt gaagtttact
     9781 tatgttatta gcaatctcta ttttttcttt tccattgtct tctaattgtt gtttcgtctc
     9841 ttcaattcgt tctttatgaa tggttgtttc taaaccttta ttatgatgta ctaatttctt
     9901 gactgtcatt ggtgtttcat actgaagttt atgaaaatca tgctcagctt ttttttgttt
     9961 ctccggacta tctttgatag attcccagtc ttcaccttgt tttaaaagat aaaatttgcc
    10021 gtctttttca agtatttttt gttctttatg gataggatag aatttaccgc ctaaattaaa
    10081 cagttgttga tcttttccta tcacgtaatg cgcatcattt aatgatgtta ctttctctcc
    10141 tttctcatta aaaaaggatt tttcttttct atgtactgca agcatgtctt gtagatttgc
    10201 caattttaaa tttaatccat tatgtttatg cagcaatttg gtggcttcct cattttcttt
    10261 tggatctgtg ctcataataa gatcatttat tgtttccaat tgtttttcca tttccaaact
    10321 aagcttatga attagagctt ccatcttgat ggttgcaaga tcatgtttag attggctata
    10381 gtctttttgg gctttttcta atgctgcggg atcattttta atggcatccc attggccttt
    10441 ttgcaggaga tagaattgtc ctttatgttc tactacttcc tggtctttat taatagccag
    10501 gtgcgcgtct tttaatgagc tgaccgggtt tcctttttcg ttaacatagg attttttacc
    10561 tgtatgagcg tcatgtaaaa cttgcaggtt agccagtttt gcttttatgt catgatgctc
    10621 acgctgtaag gattgaacgg ttttctcatc gcctttatgc ttttccatca gttgcgtgag
    10681 tttctcactg tctttgctga aggctttcat tttttcatta atttcggata gtgataactc
    10741 ttcagagtca gtgagaagtt tttcaaatcc ttccaaattt tcttcatagg ttttgtactt
    10801 gtcttcaaga tctttgcctt gacgttccag cttatccagt tttttttcta attcttcgcc
    10861 tttcttaata ttttcttgag cacgtttaat agcttgttca tattcatcca gcattttttg
    10921 aatttcttta tcagcttgtg aagtggatgg agtggactgt tggcttggct tgcttgcttt
    10981 ttcagcattt tttatggctt tttcattgta ttctcgaata atttccttga ctttttccgc
    11041 agcatgggat tttttggata aataccacaa aaacaaagcg gccattaatc ggcgtttaaa
    11101 gagctcatgc tccattctgt cttgttgatt ttgtagattg atgtttcttt gcagagccat
    11161 ttgggcacct aactcagcta ttacgtcttt acccgccgga gataaaagaa attttttgac
    11221 ctgggatgcg ttttttaacc cttctcggcc cagattaatg gtggaacctt tgtcttcctt
    11281 agcctgggga tcaagcaagc tggattctat ccatttctca aaagaagttt taaagggctt
    11341 ggctggctca ttgggttgag attctttttt agtggtgggc attgatagct tatcttcttt
    11401 tttactatcc tcaacacgca ttttttctac aacagcttcc ttttccttgg tttttacgga
    11461 ttcaagactc ccttctgatg ttttaacttg atgtgatttg ttatcttttg ccataaatag
    11521 gatccttaat caatcgcccc cagttatctt atattataca caatgttgac caataaagca
    11581 tcagtttatt catgttttaa caggattatg ccatttacta tcgtgcgatc ttgagtttgc
    11641 aactcatgca tgattggagt ctttttttgc tgaatattag tatttcatct tagaagcaag
    11701 tagcataatt tgcaaataaa aaccatgaaa tcaatgcaat tagatttgga agtgtttaga
    11761 tagtcagtgt atgttcttga ggcattgtgt ctgatgtttg tgtggcacca tgaaacagac
    11821 ctggaaaatg cagttgttgg gatttctgat tatctttgac tttattggtg gagctctttt
    11881 cgtccaataa taaattacgt aacaggcagt gactgtctga gtgaggaatt aattcgcaac
    11941 gtctgggatc attgaaatag tattgattat cctgcactat tttatgtgtc agtatatcct
    12001 catcatggaa tgcccattga tatacttttc cacgatttat ttctattaag gaaagcaaat
    12061 attgctttaa ttcacccgct ttgctgtcta tgtggttaca taatatagtc accccatacg
    12121 cgcttgttga gaacaaaact cctgctgaat gatcccaaat ctgtccgaag tgataaagga
    12181 tagtaccggt agaataggca agaagtcggg aaataggatt aataatagat aaatttactc
    12241 ttattgttag tgaaagtatg tctatcagag ggagaatcaa aatattttta atcagctgaa
    12301 ggaggggtaa aaaaagtagc cttatgacat taatgcctat gtttgtgact ggattatctg
    12361 aaaaatggcc aaagggggaa gtaaatccat aaagagggga aagcgtaaat tcagatatga
    12421 agttatttgt ggatggacgc tctgctatat cgatgagtaa tttcccaatc gcactgaagt
    12481 tacctaatac agacagtaat ttgcggaaaa aattggtaat tcctctaaaa gggacgtgta
    12541 ttaaaagcga actagcctca agtaagggga tggttaaaat agctaaaata agatcaaaac
    12601 ttgcggctaa agtttgttta cccagttgtt ttatcaattt gccacttttt ttccagccat
    12661 ttataaatcc atcaaaaaaa ccatagtaaa aaccttcaaa aaaaggtgca accaataatt
    12721 ttccaatatt tataatattc ttgcaaaacc acttgcaagt cccaaggaac cagtcatcac
    12781 ccgggtgcat aatggtatca tagatagcgg cacctccttt acctccgaga gcagcataat
    12841 tggtataagg aaagtcaccc atttcatgtt gcagtgaagg aatcgctttt gttaaaccat
    12901 aaccaaggct aagcgctaaa gaaacaataa tagcaatttc tgctgggtct tctttcaaga
    12961 cagagacagc atcgacaaag aacttatcca aattaccgct cactaccata ccttgccaga
    13021 gagttgcaga agcagagata gcttctgaaa tcttgccatg attcatcagg cgagctaatt
    13081 tttgaacagg ttcaattcca taaatcaagc cacttaagtg cagctttttt aaaatgtcag
    13141 tgagaacatt gggtgcgagt acagcacctg cgccatagac ataagctgcc atggcaagag
    13201 ttccaatcat ggggttacgc tcgctggtgt ctataaaaaa tccagctaaa tggcgtacta
    13261 cgcccaaagc tctgtgcaaa ggattttcat aggtgtgcac agtaggggga tgattcgctc
    13321 ttgcaatgac ataggggctg ttgttgatct gatcactaat gaactcttca agagtttgtc
    13381 catttgcgag aagtccctga ttttggagcg cttcatgcag agaggttctt ttaatttttt
    13441 tggattttgg acgctgatct ctttctgaaa gatgttgttt aggggcatga gttgcatgca
    13501 catgattaaa aaattcttca tgcaggtcat gtatgatgaa ttggctcatg cttttgtaaa
    13561 aacctttaaa aaaatcaggt atacgggaaa taaaagcttt gaaacggagg ctgattcttt
    13621 caggcaagta aagatgtctt ttgctgattt ggcttaaaat atataaatcc tctccatccg
    13681 gcaaaggata aaagagttga tcatgtataa aatatttgag atgaatcagt ttattttcga
    13741 gtcttaacag aacagtattt ttttgaggca caataaccca attaccatta ggtaagtggc
    13801 ttagtaatct tttcgcctct tttaaactgt ttacctctgt ctgttgggct gagttttcca
    13861 ctagatgatt taccatcgca gccagactat tataacctat ttgaggcagt cctttgagtt
    13921 tgtagtaagg cgttattttg gtaatagata ctccatcaga gcgtttttgt atttcagttt
    13981 tatgacttgg ttttgttgtg actgccagat aattaatcca atcgtgaccg tcatgccaaa
    14041 gtttacttat gaatcgttcc agttttgcaa agcgattggc cgggatgttg tttaatgctg
    14101 gcgcagggca gagttggtat tgctggcaaa ttaccctgag cgcatcgtca agctcacggc
    14161 tactgtgccc aatatggatt tcactatccc aaacaaaaca aggagtaatt ggttttccaa
    14221 gttgtgtttt agtcactttg gtaaagtcca tttgatcctg gcgagaaaca gaaaaaactc
    14281 ggttaaaccg ataatgctgt gctgttttgt gcgcatcata tagcatataa ccaaaacttc
    14341 cttctgaaac cacaggatta ttggctatca ttcgattgtc gttctggcaa tgttcaataa
    14401 tggaaatgat ttttcttctt aactcctcct gtccagcatg atcatgaggt tttaattgtc
    14461 tttctttttt caatagatgg tttaaggaac ggatacaagc attagccagg ttaatttttt
    14521 cttcctgctc atggcttagg ggttttttat agctttcagc ttgaacttta tagcgattga
    14581 cgtgatttat aacatcagca accagcgagg atggtctgtc tcccggaaga atttttaaca
    14641 gttcagggtt atgagtgaag agcatcagct gaactgtttt cttgttgtga ttggctatta
    14701 agcgtagtct gtctcctcca ggaaggagat attccatatt ataattacca taaaatactg
    14761 taccggcttc gtgactgaaa atacttccgg tgatagttgg catcgctttg tgtgtgtgcg
    14821 gagtaactct gagtggaata ataacttgag tttggccatc agtagtttca aaatcaggca
    14881 taaagattta tttggtttaa tatatggtaa aaggatactg tcattaattt agtgattttt
    14941 tatcgct
//
LOCUS       DATNSN010000084        14639 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.084, whole genome shotgun sequence.
ACCESSION   DATNSN010000084 DATNSN010000000
VERSION     DATNSN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.084"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..642
                     /locus_tag="VAH76_001948"
     CDS             <1..642
                     /locus_tag="VAH76_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO1386557.1"
                     /translation="SKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFD
                     DLFSALKNREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGK
                     KIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAKYWMASE
                     PYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF"
     gene            complement(690..2399)
                     /locus_tag="VAH76_001949"
     CDS             complement(690..2399)
                     /locus_tag="VAH76_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO1386558.1"
                     /translation="MDKRWFEQYQNGVPHEIDASQYLSLISLFNESCNSYASRAAYSN
                     LGTEITYAELDSLSRNFAAYLQQLNLEKGARVAIMLPNVLQYPVAIFGVLRAGYVVVN
                     TNPLYTADEVIHQMNDSGAEVIIVLANFAKTIEKALPSMPSVKHVIVTQLGDLFPSFK
                     RFIVNFVVNHIKKLVPAFTIPHAVAFNYALSEGKQATLHRVELNHDDIAFLQYTGGTT
                     GVAKGAILTHGNLIANVMQAYTWISPLGVSDQDIIVTALPLYHIFSLTANCLTFMKIG
                     AKNILITNPRDMGHFIDQIKNSGFTAITGVNTLFNALLNHPKFKEIDFSKLKLALSGG
                     MALQKSVSLKWSEMTKTRVLEAYGLTETSPAVTINPMYLEGYNGSIGLPLPSTDVSIR
                     DESGKEVPIGTSGELCIKGPQVMPGYWKRPDETALVFTKDGFLKTGDIARMDEEGFIY
                     LVDRKKDMIVVSGFNVYPNEVEQIIGMHPGVIEVGVVGVVDEESGERVKACIVRKDST
                     LTAEQIINHCKEHLTAYKIPKVVEFFDELPKTNVGKILRRALKNQEKEQKSSANKKTM
                     EPV"
     gene            2471..3166
                     /gene="minC"
                     /locus_tag="VAH76_001950"
     CDS             2471..3166
                     /gene="minC"
                     /locus_tag="VAH76_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEO1386559.1"
                     /translation="MGENITNTQAFKLKGRLYTLTVLQVLNTDKDAFSQQFAEIVIRA
                     PKLFERTPVVFDLSPVNHLEFDLQELCQIARNHGMIPLAIQGGSPLHDTLAQCQGLGI
                     LNASTAHDKPIIEEPSECQSIENFKTKLLTTPVRSGQQVVAKGADLIVTSSVSHGAEL
                     LADGNIHVYGTLRGRALAGISGGIETRIFCQSLDAELVSIAGFYRISDAIEPVNGPCQ
                     IYLVNEHIVVEPL"
     gene            3163..3891
                     /locus_tag="VAH76_001951"
     CDS             3163..3891
                     /locus_tag="VAH76_001951"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEO1386560.1"
                     /translation="MIDSVFISDLHLHPEDNEIQTRFDHFVNWARLSVKRIYILGDFF
                     HAWSGDDSINAWSTAIAEQIESLTQSGIEIYFMTGNRDFLLGPLFAKRAGWKILTEPS
                     VITLGNNRVLLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVNGVRKI
                     SLKNQHKSIEQMDIVSEAAIQHMNQNCTETLVHGHTHKPGITNYIHEEKTLTRYVLSD
                     WDDIPKLLCYDNTKGFYFTQYDIN"
     gene            3888..4679
                     /locus_tag="VAH76_001952"
     CDS             3888..4679
                     /locus_tag="VAH76_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO1386561.1"
                     /translation="MRSYPMSKPSNKMKEELEQIRKTEIENAMAKEREEVLAQRNRER
                     AEADDNLKKGRYIKDPRTGQSITLWESAVQKAEEVLNADLNSYMDARAAIMSLLKMYY
                     EMVKANTQTMKELRAGIGNTIMDWGVFPIKDYIGKKLTGNPEIDLPILQHEVSYTDDN
                     KLKIEPLTRSDKGLTLSEEDEKLMKEGKIQKGQIDRIFEGMIRMWLKNNDYTPDPNNP
                     GQFVNSHGQTLDKATFDQLKKDDQHGLNHFLTEHDPDLQYRPSGP"
     gene            complement(4766..5389)
                     /locus_tag="VAH76_001953"
     CDS             complement(4766..5389)
                     /locus_tag="VAH76_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEO1386562.1"
                     /translation="MLKRLDSDLQTLNDFLKVKTPKAWLQHAAANIPLLLLDHAHCER
                     KAAGTAINFISKYPEKAELVAIMAPLAREELLHFEKVIGIMKQKGIVYAPLQPSDYAS
                     NLHKHVTNKDGIERLCDQLIIGAIIEARSCERFNSIIPYIEDQDLARFYKTLVKSECR
                     HFEEYLYLAKFYGGPIDDRISEFLTIENTFILSDDMVFRFHSGLPPA"
     gene            5463..6347
                     /locus_tag="VAH76_001954"
     CDS             5463..6347
                     /locus_tag="VAH76_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386563.1"
                     /translation="MDEIKKDDELSQWLSTYGTITAERILGRYNISLPQDELLEAINI
                     PSSFYRHLLQIPLKNVLNGIVIQQASDYHVYAQKLLIDYLLSGESSKEPDSQGAGTRE
                     SLEDERQRLVQLGDEFHKLELEQDNLIASSQASLMKISIDWNAKLETTLSKLNNLYKN
                     TNSKIKKNAIRKALIKAFIHCDLVKDQSPNNKHLLIDKLNQTLAVSVSAELKEVILTN
                     LSELFQILDALNTKLDEFTDRTNHLSQQAKSFRTQFYEVILRIIELIKLLPEYKIDPE
                     QDAINREPLYFDRTIGEH"
     gene            6496..6921
                     /gene="ndk"
                     /locus_tag="VAH76_001955"
     CDS             6496..6921
                     /gene="ndk"
                     /locus_tag="VAH76_001955"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO1386564.1"
                     /translation="MAKELTLSIIKPDAVAKSVIGEIYTRFEKAGLDIVAAKMTQLSR
                     EQAENFYDIHRARPFFKDLVDFMISGPVMIQVLKGENAVAKNREIMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLENAAREIAFFFEPHELCNR"
     gene            6942..8090
                     /locus_tag="VAH76_001956"
     CDS             6942..8090
                     /locus_tag="VAH76_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HEO1386565.1"
                     /translation="MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRD
                     FAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEAN
                     RGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKIT
                     NVVMMGMGEPLLNFDNVVSAMSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVA
                     LAVSLHAPTDELRNELVPINKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQP
                     EHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDKLIKHGINTITRKTR
                     GDDIDAACGQLAGEVKDKTTRSQRWQKLHFMSKTDKSTELTISSEEIA"
     gene            8268..9050
                     /gene="pilW"
                     /locus_tag="VAH76_001957"
     CDS             8268..9050
                     /gene="pilW"
                     /locus_tag="VAH76_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEO1386566.1"
                     /translation="MLKSIRYLLTIIICLFVGACQHTQDNEEPNTIRKVNLSKAASFN
                     VQLGLGYLKQGDRPRAKKKLLTALEQQPDSADVNAALAYYFEQTKELEQAKKFYHKAI
                     TLSQNGGAQLNNYGAFLCRQGDYKNAETYFLKAVKDQNYVHTSGAYENAGLCAMAIPD
                     HDKAILYFTKALNQDPSRKESLYELVKLQSKIGHDKEAVELLQKHADLVLNDKVMLAL
                     AKDIANRTGQHSLAAEYENSFNKLEPTNNISGVNDEYNSSNG"
     gene            9025..9594
                     /locus_tag="VAH76_001958"
     CDS             9025..9594
                     /locus_tag="VAH76_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1386567.1"
                     /translation="MNTIAAMDETRNNEFNNPGYELAALRQQKGYSIEYVASKLHLRA
                     RIIELIEAGDFDLLPQPVFVKGYLRAYSKLLGVSPEPFLTVFNAQYCFEKKSERAALW
                     QSKKESHKAEHFIKWFTILFAIGVLVAVGIWWQKNKDSQAIDTPKESASGLSISETSS
                     EIKLTDLSKMESLLTPNNAQMTPLEKNGG"
     gene            9584..10864
                     /gene="hisS"
                     /locus_tag="VAH76_001959"
     CDS             9584..10864
                     /gene="hisS"
                     /locus_tag="VAH76_001959"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO1386568.1"
                     /translation="MVVDKIQAIRGMNDVLPDSTSVWRFIEQTFINCLVRYGYKEIRF
                     PIVENTQLFKRTIGEITDIVEKEMYTFNDLNGDSITLRPEGTAGCVRACIEHGLLHNQ
                     QQKLWYLGPMFRHERPQKGRYRQFNQFGVEALGITGTAIELELISICRRLWADLGFSQ
                     SVQLQVNSLGEINERQKYRSILVEYLRDHFHILDEDSKRRLDKNPLRVLDSKNPDLQQ
                     LIQNAPKLIDVLGDDSKEHFQSFCNGLETLGIPYSINPVLVRGLDYYGQTVFEWVTDQ
                     LGSQATICAGGRYDMLVEFLGGAPTPAVGFALGLERIFLLMETLNLLNESNNKQSIFI
                     IATNEEAILKALVMAESIRNAHPSLDVITNTAGGGFKSQFKKADKSGARLALILGEDE
                     IAREYVSIKDLRTEIEQISIPMTKINEFLQDYLA"
     gene            10882..11556
                     /locus_tag="VAH76_001960"
     CDS             10882..11556
                     /locus_tag="VAH76_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEO1386569.1"
                     /translation="MSVYMTEEEQLEIIKKWWKRYGNMITIFLSIMLLIIAGYRYMNW
                     HNDKIKQQASIAYENMMIAFSNQNIKSVRSYANELIKNYNHTVYADIAHMTLAKIYVS
                     KNKLEQARNELKAVASESQMTPLRQIAKIRIARLLAADKAYTNALNELSAVEDEAYLP
                     VINELKGDIYSAKGQYQDAMNAYRLAIDEVRTNGMGNLFLEMKTNELAIKTQSMITDD
                     KKVKAA"
     gene            11581..12735
                     /gene="bamB"
                     /locus_tag="VAH76_001961"
     CDS             11581..12735
                     /gene="bamB"
                     /locus_tag="VAH76_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEO1386570.1"
                     /translation="MKIRILVLILCALTQGCTYVDDYMLGKDNTPQPKELKEIQPKVK
                     MAQSWTTPVGKAHKTNEYLNIKPAIRGDIIYTADASGLVQAVNRKDGQIKWSTALKNN
                     IVSGPTVAAGYVVVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTID
                     GKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRL
                     IWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPAS
                     VYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKQTSLKARGLTAPALVGGNLAVGDK
                     AGYLHILSTQTGELLGRSQLSGGVTVSPSVSGKNMYVLTNNGMLNQLSVS"
     gene            12740..14128
                     /gene="der"
                     /locus_tag="VAH76_001962"
     CDS             12740..14128
                     /gene="der"
                     /locus_tag="VAH76_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO1386571.1"
                     /translation="MIPVIALVGRPNVGKSTLFNRITKTQDALVADFPGLTRDRQYGH
                     AQHENKSFIIVDTGGIGVDDIEVDTLMSRQSQVALNEANVILFLVDGRSGLTGIDQQI
                     AQALRKLNKKVHLVVNKTDGMNEDIACADFQSLGITDVHAISASHGGGISSLLEEILE
                     PFTTETHEATDDKAIKIAFAGRPNVGKSTLINRILGEERVVVYDMPGTTRDSISIPFT
                     REDKQYVLIDTAGVRRKSRIDEKIEKFSVIKTLQAIKEAHVCLLLLDANEGITDQDMN
                     LLGFIIESGKALVIAVNKWDGLEEDHKEKIKSELSRRLHFANFAKIRFISALHGSGVG
                     GLFKDINEAYHSAIQSFSTPKLTRLLQDISAKHTPPCINGRRIKLRYAHLGGHNPPVI
                     VIHGNQLDALPESYKRYLNNEFIKHLGLVGTPLKIEFKGGQNPFANKKNKLSQRQVNK
                     KKRLMRWAKSKK"
     gene            complement(14204..14626)
                     /locus_tag="VAH76_001963"
     CDS             complement(14204..14626)
                     /locus_tag="VAH76_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO1386572.1"
                     /translation="MYTNILFATDLLNEHSHLTEKAANIAKQFNAKLYLLHVIELPTS
                     ILIAQGLGFTELANPSKEDAQTVLSLIGESLQIPQEQQFVEIGSVKEHILNKAKDLNC
                     QLIIIGSHSATGLPSILGSTAHAVVNHSVCDVLTLKAD"
BASE COUNT         4951 a         2542 c         2826 g         4320 t
ORIGIN      
        1 tcaaaattta atccgccttt tgaagtttgg tctggtaaca acagttcatt gtatggcttt
       61 gatattgacc ttatgcaaga aatatgcaga agattacatg cgacctgtac ttttgaagcc
      121 tatatattcg atgatttatt ttccgcatta aaaaaccgcg aagtcgatct ggttattgcc
      181 tcaatgatta ttacagatga aagaaagaaa cactttattt ttagtcttcc ctatatggaa
      241 agtaactcac aatatataac taccgtcgat tctaaaatca gcacatttga tgatttgcac
      301 ggcaaaaaaa ttggagtaag aaaaggaact ccttacgcaa ggcaagtttt gtcagaaaac
      361 cgcaacaatc aggtaatttt ttacgagctc atacaagata tgcttcttgg cttaagcaat
      421 aatgaagttg atgccagttt aatggattat gaagcagcaa aatattggat ggcatctgaa
      481 ccctatgctt ataaattaat tggtaagaaa atttctattg gagaagggta ttctattatg
      541 gcaaaccctg atcaatttgt attaattaaa aaaataaata aaattcttct tgaaatggaa
      601 gctgatggaa cttatctgag actatattca gagtactttt agaagaggag tattaaggac
      661 aaatagcacc caaaatactc caattatacc taaacaggct ccattgtttt tttattggcc
      721 gagctttttt gttccttttc ttggtttttt aaagcacgac gtaaaatctt gcccacattt
      781 gttttaggaa gttcgtcaaa gaattcgaca actttaggga ttttataagc ggtcaaatgc
      841 tccttgcaat gattgataat ttgttcagcc gtcaatgtac tatcttttct tacaatacag
      901 gctttaaccc gttcaccaga ttcttcatca acaacaccta caacacctac ttctattaca
      961 ccgggatgca ttcctattat ttgctctacc tcatttggat aaacgttgaa tcctgaaaca
     1021 acaatcatgt ctttttttct atcgaccagg tagataaagc cttcttcatc catacgggca
     1081 atatcaccag tttttaaaaa cccgtcttta gtaaatacca aggctgtctc atcaggcctt
     1141 ttccaataac caggcatcac ttgtggtcct ttaatgcaca actccccact tgtaccaata
     1201 ggcacttctt tcccggattc atcacgaata gacacatcag ttgagggaag aggtaagcca
     1261 atactgccat tatacccttc aagatacatt gggtttatag tcacagcagg gctggtttca
     1321 gttaatccat aagcttccaa aacacgtgtt ttggtcattt cagaccattt taaggacaca
     1381 ctcttttgca atgccatacc gcctgataat gccagtttta atttgctgaa atcaatttct
     1441 ttaaatttag gatggttcag tagtgcattg aatagagtat ttaccccggt tattgcggta
     1501 aaacccgaat ttttgatttg atctataaaa tgtcccatat cacgtggatt ggtgatcaga
     1561 atatttttcg cgccaatttt cataaaggtg agacaattgg ccgtcaagga gaaaatatgg
     1621 tacaggggaa gtgcagttac tataatgtct tgatcgctga ctcccaaagg agatatccag
     1681 gtgtatgctt gcatcacatt ggctataaga ttgccatgag tcaaaatagc gccttttgca
     1741 acaccagtag taccgccagt atattgtaga aacgcaatat catcatgatt cagttcaact
     1801 cgatggagtg ttgcctgttt cccttcagac aaagcatagt taaaagccac ggcgtgagga
     1861 attgtgaagg caggaactaa tttttttata tgattcacta caaaattaac aatgaatctc
     1921 ttgaatgaag gaaataaatc gcccaattgc gtaacaatca catgcttaac ggaaggcatt
     1981 gacggcaatg ctttttcaat cgtttttgca aaattagcca gcacgataat gacctctgcc
     2041 cccgagtcat tcatttggtg tattacttca tccgctgtgt ataaagggtt agtgtttacc
     2101 acgacataac ctgctcgtaa cacaccaaag atggctacag gatattgtaa aacattaggc
     2161 agcattattg ctactcttgc ccctttttcc aaattcaatt gctgcagata agcagcgaaa
     2221 tttctgctta aactgtccag ttcagcatac gtaatttcgg tgcctaaatt ggaataggca
     2281 gctctgctag cataagagtt acatgattca ttaaataaag atatgaggga taaatattga
     2341 cttgcgtcaa tttcatgtgg tactccattt tggtattgtt caaaccatcg tttatccact
     2401 ttgatatcct tctgttattg ttttcctcat gatagtataa acaaaaaact ctctaatgga
     2461 tatcatgcta atgggtgaaa atataactaa tacacaagca tttaagctta agggaaggtt
     2521 gtatacttta accgtattgc aagttttgaa cactgataaa gatgcttttt ctcaacaatt
     2581 tgctgaaatt gttattagag cgcctaaatt atttgaaaga acgcccgttg tttttgactt
     2641 atctcctgtc aatcatctcg aatttgattt acaggaatta tgtcaaattg cacgtaatca
     2701 tggtatgatc cctttggcaa ttcaaggtgg tagtcctttg cacgacactc tggctcaatg
     2761 tcagggtttg ggcatactta atgcctcaac agcacatgat aagccaataa tcgaagaacc
     2821 ctcagaatgt caaagtatag agaatttcaa aacaaaatta ctaacgacac cagtgcgttc
     2881 aggccaacag gttgttgcca agggagctga tttaatagtg acttcttcag ttagccatgg
     2941 ggcagaatta ttagctgatg gaaatattca tgtgtatgga acattacgcg gcagggcatt
     3001 agcaggaata tccgggggta tcgagacaag aattttttgc cagtctttgg atgctgaatt
     3061 agtatctata gcgggttttt atcgaataag tgatgctatt gaaccagtta atgggccttg
     3121 tcagatctac ttggtaaatg aacatatcgt tgttgaacca ttatgattga ctccgttttt
     3181 atatccgatt tacatctgca tccagaagat aatgaaatcc aaaccagatt tgatcatttt
     3241 gtaaattggg ctcggctatc tgtaaaaagg atatatatcc tgggtgattt ttttcatgcc
     3301 tggtcaggtg acgattcaat taacgcatgg agtactgcta tagcagagca aatagaatca
     3361 ttgactcaaa gtggcattga aatttatttt atgaccggaa atcgtgattt tttacttggt
     3421 ccattatttg ccaaacgagc cggttggaaa atattaacag aaccaagtgt tatcacactt
     3481 ggaaataaca gagttttgct ggttcatggc gatcgttatt gtactaatga cagatcacat
     3541 cagcgattcc gattattaac gagaaacagg tggttctcat ttctcttttt aagacttcct
     3601 ttatcgtttc gcaaacgatt ggttaatggg gttcgcaaga ttagtctaaa gaatcaacat
     3661 aaatcaatcg aacagatgga tattgtttca gaggcggcaa ttcagcatat gaatcaaaat
     3721 tgcacagaaa cactagttca tggacacaca cataaacctg gcataacaaa ttacattcat
     3781 gaagaaaaaa cactgaccag gtatgttcta agtgattggg atgacatacc taaactattg
     3841 tgttatgata atacaaaagg attctatttc acccaatatg atataaattg aggagttacc
     3901 caatgtctaa accatcaaac aaaatgaaag aggaactcga acaaattcga aaaactgaaa
     3961 tagaaaacgc tatggcaaaa gagcgtgaag aagtccttgc tcaaagaaac agagaacgcg
     4021 ctgaggccga tgacaaccta aaaaaaggtc gatatatcaa agatcctcgt acaggccaat
     4081 caataactct ctgggaaagt gcagtgcaaa aagcagagga agttttgaat gctgatttaa
     4141 attcttatat ggatgctcgt gccgcgatca tgagtttatt aaaaatgtat tatgaaatgg
     4201 tcaaggcaaa cactcagact atgaaggaat tgcgcgcagg aattggtaac acgattatgg
     4261 attggggtgt ctttcccatt aaagattata ttggaaaaaa actcacggga aatcctgaaa
     4321 ttgatttacc aattttgcaa catgaagtaa gttatactga tgataataag ttaaaaatag
     4381 aaccgcttac ccgttcagat aaaggactaa cgctctcaga agaagatgaa aaacttatga
     4441 aagaaggaaa aattcaaaaa ggtcaaattg atcgaatatt tgaaggtatg atccgcatgt
     4501 ggctaaagaa taatgattat acaccggatc ccaacaatcc tggccaattt gttaattcac
     4561 acggtcaaac attagacaaa gcaacatttg atcaattaaa aaaagatgat cagcatgggc
     4621 ttaaccattt cttaacggaa catgatccgg atttgcaata cagaccaagc ggaccttaat
     4681 taagcaaaag gaatatcgag taacactagc ctacataata cttataggct agtgttgaaa
     4741 agtctgttga ctctgcttta aggcattaag caggaggcaa gccactatga aagcgaaata
     4801 ccatatcatc tgataaaata aatgtatttt ctatagttaa aaattcagaa atacgatcat
     4861 caatagggcc tccatagaac ttggccaaat acaaatattc ttcaaaatgc ctgcattctg
     4921 atttaaccaa tgttttataa aacctggcta agtcctgatc ttcaatatat ggaattattg
     4981 agttaaaacg ctcgcatgat cgagcctcaa tgattgcgcc tataattaac tgatcgcata
     5041 gcctctcaat tccatcttta tttgtgacat gcttatgtaa gttcgatgcg tagtctgacg
     5101 gctgtaaggg agcataaaca attccttttt gcttcatgat accaatgacc ttttcaaagt
     5161 gtagtaactc ttctcttgcc aatggcgcca taattgctac taactctgct ttttctggat
     5221 atttacttat gaaattaatt gctgttccgg ccgctttacg ttcacaatga gcgtgatcga
     5281 gtaataaaag aggtatatta gcggcggcat gttgcaacca tgctttggga gttttaactt
     5341 tcaaaaaatc atttaacgtt tgtaaatcac tgtctaatcg ttttaacatg ataaaataca
     5401 ctataatttc aaataaatag cccttatatc acatacttta aagaatttaa attggataaa
     5461 aaatggacga aataaaaaaa gatgatgaat tgagtcaatg gctttctact tatggaacaa
     5521 taacagctga acgaatatta ggtagataca atatatcact gccgcaagat gaactattgg
     5581 aagctattaa catcccgtct agtttctata gacatttgtt acagattccc ttaaaaaacg
     5641 tacttaatgg aattgtaatt caacaagcaa gtgattacca tgtctatgca cagaaactgt
     5701 taattgatta tttgttatct ggtgaaagca gcaaagagcc tgattcacaa ggagccggaa
     5761 ctcgcgaatc actggaagat gaaagacaga gattggttca gttaggggat gagtttcata
     5821 aattggaatt agaacaggat aatctcattg catcaagcca agccagctta atgaaaataa
     5881 gcattgactg gaatgcaaaa ctggaaacaa ctctttccaa attaaataac ttatacaaaa
     5941 atacaaactc taaaattaaa aaaaatgcaa ttagaaaagc tttaataaag gcctttattc
     6001 attgtgatct agttaaggat caatcaccaa ataacaagca cctgctaatt gataaattga
     6061 atcagacatt agctgtttca gtaagtgctg aattaaaaga ggttatcctg accaatctga
     6121 gcgaactctt tcaaatcctc gacgctttaa atacaaaact cgatgaattt actgaccgga
     6181 ctaatcatct aagtcaacag gctaaatcat ttagaaccca attctatgag gtaatacttc
     6241 gtatcattga gttaattaaa ttactgcctg aatataaaat tgacccggaa caagatgcga
     6301 ttaatcgaga accattatat tttgacagaa cgattggaga acactaacgg ctttaattag
     6361 aataaaaccc tatttttgtc taaaatcata caattactat taagtgaata gatgaatgca
     6421 tgaattttta ttagccagac cactatgaat tcatgtataa taatgcactt taaattaatc
     6481 tcattggagt gcatgatggc aaaagaatta acattatcta ttattaaacc tgatgctgta
     6541 gcaaagtctg tgataggtga aatatatacg cgctttgaaa aagcaggcct tgatattgtt
     6601 gctgctaaaa tgacacaact ttcgcgcgaa caagccgaga acttttatga tattcatcgc
     6661 gcacgaccat tcttcaagga tctggtggat tttatgattt ctggtccagt gatgatccaa
     6721 gtattaaaag gtgaaaacgc tgtcgctaaa aacagagaaa ttatgggtgc gaccaatccc
     6781 aaagaagcag cacctggaac tatacgtgcc gattttgctg atagtattga tgcaaatgct
     6841 gtgcatggtt ctgacagcct tgaaaatgct gctcgtgaaa tagcattttt ctttgaacca
     6901 catgagttgt gcaatagata aagacaggct tgggagcggt aatggaccaa caaaaagtga
     6961 atctattaaa ttataattac tcgcaactac gagaattatt gatggcgtgg gatgaaaaac
     7021 ctttcagagc tcaacagcta tttcaatgga ttcatcaagt tggcatacgt gactttgctc
     7081 agatgactaa tttagggaaa gtgttaagaa ataaactttc ccaattagct tgtatagatt
     7141 tacctgaaat cgttgcatgt caaaaatcag ctgatggcac acataagtgg ctattaaagc
     7201 tggagtgtgg aaattgtatt gaaaccgtat tcattccgga agcaaatcga gggacacttt
     7261 gtgtttcatc acaagtaggt tgtgctttaa attgttcttt ttgctccaca gcaaagcaag
     7321 gatttaatag aaatttatca acagctgaaa taattggtca agtatggttg gcagcacgag
     7381 aactatcaga taataatggc gctcacgata agaaaataac taacgtcgtg atgatgggaa
     7441 tgggtgaacc attacttaat ttcgataatg ttgtttctgc tatgagcatc atgatggatg
     7501 atttagccta tggattatcc aaaagacggg ttacattaag tacttcgggt gttttaccag
     7561 aaatggaacg actcagagaa gtcagtcctg tcgcgttggc ggtatcctta catgcaccta
     7621 cagatgaact acgtaacgag ctggtaccaa ttaataaaaa atatcctctc tcccaattaa
     7681 tatccctatg taaacgatat tttaaagacg aaccaagaag aaaagttact tttgaatacg
     7741 tcatgctaaa aggggttaat gatcaacctg aacatgctag tcaattaatt aaactattgc
     7801 ataatgtgcc tgcaaaagtt aatttaattc cttttaaccc atttccttta actcaatatc
     7861 agagatcatc gcgggaaaca attgatgcat ttcgagataa attaatcaag catggtatta
     7921 atactattac tcgcaaaact cgtggagatg atattgatgc agcatgcggc caacttgctg
     7981 gcgaggtaaa agataaaacc accagatcac aacgatggca aaaacttcat ttcatgagca
     8041 aaacagataa atcaactgaa ttgaccattt cttcggaaga aattgcataa cacagtcttg
     8101 tataataact gagagccaaa taagaacttt ctccaatatt cctggttgga gattatgtat
     8161 aaaaggtatt ttcacctaca gactcacatt aaatagtgac aaatcatttt taatttagac
     8221 ttaagtggtt ataatgcgaa atcatttcta acgtaaagtg attaatcgtg ctaaaaagca
     8281 taaggtactt attaactata ataatttgtt tatttgttgg agcttgtcag catacacaag
     8341 acaacgaaga gcctaatacc attagaaaag taaaccttag caaagcggct tcttttaatg
     8401 tgcagttagg gttgggatat ttgaagcaag gcgacagacc tagagcaaaa aagaagctgc
     8461 taaccgcctt ggaacaacaa ccagattcag cggatgttaa tgcagcatta gcctattatt
     8521 ttgaacaaac caaagaattg gagcaagcaa aaaaattcta tcataaagca ataaccctat
     8581 cgcaaaatgg tggtgcacaa ttgaataatt acggcgcttt tctgtgtcga cagggggatt
     8641 ataaaaatgc tgaaacttac ttcctgaagg ccgttaaaga tcaaaactat gtgcatacct
     8701 caggtgctta tgaaaatgct ggattatgtg ctatggctat acctgatcat gataaagcca
     8761 ttttatattt taccaaggcc ttaaatcagg atccatcgag aaaagaatcc ctatatgaat
     8821 tagtaaaatt acaaagtaaa ataggacatg ataaagaagc cgtcgaactg cttcaaaaac
     8881 atgctgattt agtattaaat gataaggtaa tgcttgcatt agcgaaagac atagcaaata
     8941 gaaccggcca acattctctt gcagcagaat atgaaaatag cttcaataaa ctggagccaa
     9001 ctaataatat tagtggagta aatgatgaat acaatagcag caatggatga gacaagaaat
     9061 aatgaattta acaatcccgg ctacgagctg gcggctcttc gccagcaaaa ggggtattca
     9121 atagagtatg ttgcgagcaa acttcattta cgagctcgta ttatagagct cattgaagca
     9181 ggagattttg atttattacc tcagccagtt tttgtaaaag ggtacttgcg tgcctattca
     9241 aaattattag gagtttcacc tgaacctttt ttaaccgttt ttaatgcaca atattgtttt
     9301 gaaaaaaaat cggaaagagc agccctatgg caaagcaaaa aggaatcaca taaagcagag
     9361 cattttataa aatggttcac cattttattt gcgattggag ttttagttgc agttgggata
     9421 tggtggcaaa aaaacaagga tagccaggct atagatacac cgaaagagag tgcatctgga
     9481 ttatcaatca gtgaaaccag ttcggaaatt aaattgactg atttatcaaa aatggaatcg
     9541 ttattaacgc ccaataatgc acaaatgacg cctttggaga aaaatggtgg ttgataaaat
     9601 tcaagctatc cgaggaatga atgatgtact gcctgatagt acctcggtat ggcgctttat
     9661 agaacagact tttataaatt gcctcgtacg atatggttac aaggaaattc gttttccaat
     9721 tgttgagaat acacagctat ttaaacgtac aattggtgaa ataacagata ttgttgaaaa
     9781 agaaatgtac actttcaacg atttgaatgg tgatagcata accctcaggc cagaaggaac
     9841 agccggctgt gttcgtgcct gtatagaaca tggtttattg cataatcaac aacaaaaatt
     9901 atggtactta ggacctatgt ttcgtcatga acgtcctcag aagggtagat accgccaatt
     9961 taaccaattt ggggttgagg cgctaggaat taccggtact gccattgagc ttgagttgat
    10021 atcaatttgc cgtagattat gggctgatct gggcttttct cagtcagttc aattacaagt
    10081 taattcacta ggtgaaataa atgagcgtca gaaatatcgc tctatcctgg tagagtatct
    10141 acgcgatcat tttcatatac ttgatgaaga cagtaagagg cgattggata aaaacccttt
    10201 gagagttctt gatagtaaaa accctgatct tcaacagctc attcaaaatg cccctaagtt
    10261 aattgatgtt cttggtgatg atagcaaaga acactttcaa tcgttctgca acggattgga
    10321 aacacttggt attccttatt ccatcaaccc tgtattggta agaggattag attattatgg
    10381 ccaaacggtg tttgaatggg taacagacca attaggaagt caagctacaa tatgtgcagg
    10441 aggtagatat gatatgctgg tagagttcct tggcggagca cccacacctg cagttgggtt
    10501 tgctttaggg ttggaaagaa tatttctttt aatggaaact ttaaacttgt tgaatgaaag
    10561 taacaacaag caatcaatat ttatcattgc tactaatgaa gaggccatat tgaaagcgct
    10621 tgtcatggca gagtcaattc gaaatgctca tccatctctt gatgtcatta ccaatacggc
    10681 aggcggtggt tttaaaagcc aatttaaaaa ggccgataaa agtggggctc gcttggcttt
    10741 gattcttggg gaagacgaaa tagccaggga atatgtaagc attaaagatt taagaactga
    10801 aatagaacaa atatcaatac cgatgaccaa aataaatgaa tttttgcaag attatctagc
    10861 ttaagggcag gagaatatta tatgtctgtt tacatgacgg aagaagaaca attagagatt
    10921 ataaaaaaat ggtggaagcg atatggcaat atgataacta tttttttatc aataatgctc
    10981 ctgattatcg cagggtaccg atatatgaat tggcacaatg acaagataaa acaacaagca
    11041 tctattgcct acgaaaatat gatgattgca ttttccaatc aaaatataaa atctgttcgt
    11101 tcctatgcca atgaattaat taagaattat aaccatactg tttatgctga tatagctcat
    11161 atgacacttg caaaaatcta cgtaagtaaa aataaacttg agcaagcaag aaatgaatta
    11221 aaggctgttg catcggaaag ccaaatgaca ccgctaagac aaattgccaa aatccgcatt
    11281 gcacgtttac tggcggcaga taaagcttat accaatgcat taaatgaatt aagtgctgtt
    11341 gaggacgaag cctacttgcc agtaattaac gaactgaaag gggatattta tagcgccaaa
    11401 ggccaatatc aggatgcgat gaatgcctac cgacttgcaa tagatgaagt aagaacaaat
    11461 ggaatgggta atttattttt agaaatgaaa acaaatgagt tagcaattaa gactcaatct
    11521 atgattactg atgataaaaa ggtaaaagca gcttaatttt attataaagg tttgtttttt
    11581 atgaagatta gaatattagt tttaatacta tgtgccttaa ctcaaggatg cacatatgtg
    11641 gatgattata tgctcggtaa ggataatacg ccccaaccca aagagctaaa agaaattcag
    11701 cccaaggtta aaatggcaca aagctggaca actccagttg gaaaggctca taaaacaaat
    11761 gagtatttaa atatcaaacc agctatacgt ggcgatataa tttatactgc agatgcaagt
    11821 ggattggttc aggcagttaa caggaaagat ggtcagataa agtggtctac cgcattaaaa
    11881 aataatatag ttagtgggcc aactgtagct gctggttatg ttgttgtagg aactaatgcc
    11941 tccacattgg tattacttaa tcaatcggac ggaaaagaga tatggcagaa taaagtatct
    12001 gctgaagtat tagctcctcc tgctatctca catcagaagg tcattgcaaa aaccattgat
    12061 ggtaaagtgt atgcaattga tgcggtaaac ggcaagcaac tgtgggttgc agatcatggc
    12121 gctcccagcc tggtgttaaa agccagctcg tcccctatca ttgtcgacga tctggttctt
    12181 gtcggatttt ccgatggcaa attagatgct ttggaattac agacaggacg attgatctgg
    12241 caaagaagca ttgcttatgg aacaggagct agtgatgtcg aacgtttagt tgatattgat
    12301 tctgacccta taataagcaa taacgtcgcc tatttggcaa cttatcaggg atatgttgga
    12361 gcactatcac tgtctaatgg ccaattcata tggagaaagc cagcatctgt ttataaaaat
    12421 atgcttttaa gtcataataa tctgtatttt acagacagca atgatgtctt gtggtctttg
    12481 aacagtagca caggacaggt caactggaag caaacctccc taaaggcaag agggcttact
    12541 gcacctgcat tagttggagg aaatttagct gttggtgata aagcaggata tttacatatc
    12601 ctatcaactc aaacaggtga gttacttgga cgttctcagc tttctggtgg agtaacagta
    12661 tcaccgagtg tttctggtaa aaatatgtat gtattaacca ataatggaat gctcaatcaa
    12721 ctttcagtga gctaataaaa tgatccctgt catcgcttta gttgggcgtc ccaatgttgg
    12781 aaagtctact ttgtttaata gaattactaa aacgcaggat gctcttgtcg cagattttcc
    12841 tggcttgaca agggatagac aatatggaca cgctcaacat gaaaacaagt cctttattat
    12901 tgttgatacc gggggcatag gagttgatga cattgaagta gatactttga tgtcaagaca
    12961 gtctcaagtt gctttaaacg aagcaaatgt gattcttttc ctggtcgatg gccgttcagg
    13021 actaacaggt atagatcagc aaattgcaca agcattaaga aaacttaata aaaaggtaca
    13081 tcttgttgtc aataaaacag atggaatgaa tgaagatatt gcttgtgccg attttcaatc
    13141 attaggcatt actgatgtcc atgctatatc tgcatctcat ggtggaggca tcagttcact
    13201 actcgaagaa attctcgagc catttacaac agaaacgcat gaagcaactg atgataaagc
    13261 gatcaaaatt gcttttgcgg ggcgccctaa tgttggaaaa tcaactttga taaacaggat
    13321 attaggggaa gaaagagtag ttgtttatga catgcccgga acaactcgag atagtatttc
    13381 aatacccttt acaagagaag ataagcaata tgtgcttatt gatactgctg gtgtacggcg
    13441 taagtctcga attgatgaaa aaatagaaaa gttttctgtc atcaaaactc tgcaagctat
    13501 aaaagaagct catgtctgtt tgttgcttct tgatgcaaat gaaggcatta ccgatcaaga
    13561 tatgaatttg cttggtttta ttattgaatc tggtaaagcg ctagttattg ctgtgaataa
    13621 atgggacggc ctggaagaag atcataaaga aaaaataaag tcagaactgt caagaaggtt
    13681 gcattttgca aattttgcaa aaatcagatt tatttcagca ttacatggga gcggggtagg
    13741 tggattattt aaagatatta atgaagccta tcattcagca atacaatcct tttctactcc
    13801 aaaactaacc agattattac aagatatcag cgcaaagcat acaccaccat gcatcaatgg
    13861 ccgacgaata aaattacgtt atgctcacct tggcggacac aatcctcctg tcattgttat
    13921 tcatggaaat caactcgatg ccttgccaga aagttataaa cgttatttaa acaatgagtt
    13981 tattaagcat ttaggattag taggtactcc tttaaaaatc gagtttaaag gaggtcaaaa
    14041 tccatttgcc aataaaaaaa ataaattatc acaaaggcaa gtgaacaaga agaaacgatt
    14101 aatgcgatgg gcaaaaagca agaaatagca ctccggttca gctcagccag atttaaattg
    14161 ttataatgaa aaaaagtatc aattgggtac taatctcttc ttattaatct gctttgagtg
    14221 ttaatacatc acaaaccgaa tgatttacta cagcatgcgc tgtacttcct agaatagacg
    14281 gtaatcctgt agcagagtgt gagcctatta taattaattg gcaattcaga tcttttgctt
    14341 tgtttaatat atgttctttt acggacccga tttctacaaa ttgttgttcc tgaggtattt
    14401 gtaaactttc tccaattaat gataatacag tttgggcatc ctcttttgat gggttagcta
    14461 gctccgtaaa acccaatcct tgagctatta atatacttgt tggcaattca atcacatgta
    14521 atagatacaa ttttgcatta aactgtttgg caatattagc agccttttct gtaaggtggc
    14581 tatgttcatt taacaaatct gtggcaaata aaatatttgt atacatgtta acctcattt
//
LOCUS       DATNSN010000085        14624 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.085, whole genome shotgun sequence.
ACCESSION   DATNSN010000085 DATNSN010000000
VERSION     DATNSN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.085"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(139..1068)
                     /gene="ftsX"
                     /locus_tag="VAH76_001964"
     CDS             complement(139..1068)
                     /gene="ftsX"
                     /locus_tag="VAH76_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEO1386573.1"
                     /translation="MLKRSQSLLAYHLQAAANSLNLFCRKPIATMMTVIVIAIALALP
                     TLFWVFTDNLSELTNRWQRGGHISLFLKSSLPEAEQTSLLEKVRTTEGVGQANIKSAA
                     DGLSELTQQEGMQDIMRYLPENPLPAVIEVVPALVIDSPAKLDLLSRKLQAFPQVALA
                     KLDMEWINRLHAILGFSGKAANALMALLALAVVFIIGNTLRLDIHNRQEEIKILKLIG
                     ATDPYIIRPFLYSGVWYGAAGALLAIFLVNIFILTLGVAVNQLANVYQMHYPLACLSL
                     RQILLLVLFAIILGWLGALLSVKRQLASIEPYK"
     gene            complement(1062..1712)
                     /gene="ftsE"
                     /locus_tag="VAH76_001965"
     CDS             complement(1062..1712)
                     /gene="ftsE"
                     /locus_tag="VAH76_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO1386574.1"
                     /translation="MITFDQVSKRYPGGFEALSQVNFSLQKGEMVFLTGHSGAGKSTL
                     LKLIALLEWPTSGQLTVNGLKLNHLKKRDVAAHRSQLGITFQSPQLLNDRTVFDNVAL
                     PLQIQGIAYPMIAKRVHAALDMVGLLSKEKMLPVHLSVGEQQRVGIARAVVHKPALLL
                     ADEPTGNLDPKLSSEIMKIFEQFNQVGVSILIATHDLALIAGMKHRIVMLKGGRLC"
     gene            complement(1699..2766)
                     /gene="ftsY"
                     /locus_tag="VAH76_001966"
     CDS             complement(1699..2766)
                     /gene="ftsY"
                     /locus_tag="VAH76_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO1386575.1"
                     /translation="MIKWFKRNQNSEPNLDTEEQQDHAESSMESRLEEPKELTVKEPS
                     PKESIFARFRKGLSKTRHQLGEGISRLLLGKKEISQELLEELETLLISADLGLDTTQD
                     VLKQLNEGLARKQLSNGEAVYQTLKTNLQTILSQEEKPLSIETEDGSPFVILMVGVNG
                     AGKTTTIGKLAKQFQKQGKKVMLAAGDTFRAAAVEQLHVWGERNGIPVIAQHTGADSA
                     SVIFDALQAAKARKIDILIADTAGRLHTQSNLMEELKKVKRVMQKLDPKAPHETMLVL
                     DASIGQNALAQAKQFHEAVQLTGITMTKLDGTAKGGILFAIANDLGIPFRYLGVGEGI
                     EDLRPFDAAQFVSAIFDDDHI"
     gene            2829..4154
                     /locus_tag="VAH76_001967"
     CDS             2829..4154
                     /locus_tag="VAH76_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO1386576.1"
                     /translation="MHKLLISLMMLLSFQAFSQVQEYTLNNGLKVLVKEDHRAPVVVS
                     MIWYNVGSADEPVGITGVSHAIEHMMFKGTSKYPVGVFSKTIAALGGQENAFTNNDYT
                     AYYEKLDAGHLATSFELEADRMNNLLLNSEEFAKEIKVIQEERRLRTDNNPQALAFER
                     FLATAHLTAPYNHPVIGWMNDLKQMKVEDLKKWYESYYAPNNATLVVVGDVNPEKVHA
                     LAERYFGSIAKRPIASRKPQQEPSALGKKMVYINAPAKLPLLLIGYTVPSVKTAKNNW
                     EPYALEIIAGILDAGESARFAKHLVRGNQVATGAEAYYNLYSRYQSQFIVYGAPSQDH
                     QIKDLEKALITELEALKKAPVSNQELQRVKNQIIAQKTFEKDSIFGQAMELGLLETIG
                     LGWKNTETYTKAINEITPEQIQQVAQRYFQENNMTVAELKPIRQEEVRP"
     gene            4151..5455
                     /locus_tag="VAH76_001968"
     CDS             4151..5455
                     /locus_tag="VAH76_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO1386577.1"
                     /translation="MSVLRAFIISFSVIFSHNLFANSFKTEKWQTKNGVRVVFYQAME
                     VPMLDISLAFAAGSAYDGKYFGLSALTTNLINQGNSGKDATTIAEALADTGAQFNAET
                     SRDMVVLSLRTLTSKEALQQSTKTFSQIISHPDFPKEAFAREKDQLLMAVEQTEESPD
                     DVAIQNFFKTLYQEHPYAHPVHGTVESLNAIKENQVIDFYKKYFVAKNGILVMVGAID
                     SSQAHQLAEQLTQDLPAGEPAPTIPKASQLADAEKINVPFPSSQTVVRLGQIGIDHHN
                     QNYFPLMVGNYILGGGTLVSRLGTEVREKRGLTYGIDSQFVPMLGEGPFIISLSTKNS
                     EARNALHITQDTLIKFIKNGPNKEELTSAKQYLTGSFPLSLGSNTNIANLLLRMAFYH
                     LPDNYLDTYVAKINAVTDAEIRQAFQQQVNPEKLLLVTVGQS"
     gene            5452..5997
                     /gene="rsmD"
                     /locus_tag="VAH76_001969"
     CDS             5452..5997
                     /gene="rsmD"
                     /locus_tag="VAH76_001969"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO1386578.1"
                     /translation="MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDI
                     RNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPNLHLIKTD
                     TLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
                     LWKQLKLKQAGQVIYGLFEKL"
     gene            6072..6563
                     /gene="dotD"
                     /locus_tag="VAH76_001970"
     CDS             6072..6563
                     /gene="dotD"
                     /locus_tag="VAH76_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HEO1386579.1"
                     /translation="MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEA
                     AVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAA
                     HFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGIKASIHVYPNSQVVELRYAK
                     IYS"
     gene            6544..7455
                     /gene="dotC"
                     /locus_tag="VAH76_001971"
     CDS             6544..7455
                     /gene="dotC"
                     /locus_tag="VAH76_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotC"
                     /protein_id="HEO1386580.1"
                     /translation="MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTR
                     AQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLE
                     HNILPPVLLEGRNTLNLADAQSIRVSDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEA
                     PNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAM
                     NMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERF
                     KNMEKLANQAKIVITNKSWQPIIAPVS"
     gene            7455..8588
                     /gene="dotB"
                     /locus_tag="VAH76_001972"
     CDS             7455..8588
                     /gene="dotB"
                     /locus_tag="VAH76_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HEO1386581.1"
                     /translation="MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPI
                     FAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYR
                     YRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGS
                     GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFAD
                     GVRNALRRKPRLIMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFS
                     GEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTS
                     ATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ"
     gene            8670..10397
                     /locus_tag="VAH76_001973"
     CDS             8670..10397
                     /locus_tag="VAH76_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO1386582.1"
                     /translation="MIFHRKCLSHFFNLFISLILGSSLNWASVNQHSTDSPTLNIKIL
                     GINDFHGQISSGRTVKNEPVGGAAILAAYIKEAQLGMEDRSIITIMGDLVGASPPSSG
                     LLNDEPSILFINSLGNNQCKPESRMNPLCNIVATVGNHELDKGQKAMFDLIYGTNNPP
                     TDSWISLPSYPGSSYPYVSANIVDAKSEQPLFPPYIIKRIHNIPIAFIGAVLKDAADS
                     MFPANAEGIKFLDEADSINRYIPEIKAKGVNVIIVLIHEGGNQIPYEGDTQTASAVEG
                     HIKEIVYSLGDEIDVVMGGHTHQFLNAFLRNRNGHEILVTQANSYSAAFAEVTLHIDT
                     RNHAVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRK
                     QNEDGESSLGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNRIV
                     IITLTGNDIYDLLEQQWMGSYINMLQISGFAYTYDSHQPLGHKIIEIRHQNKPLLREK
                     MYTIATSDFLASGNGVFSVMKRGKIIHIGQSDHDTVIAYIKSLPQPFHASIEARIKES
                     ETGLKTGVG"
     gene            complement(10439..11236)
                     /locus_tag="VAH76_001974"
     CDS             complement(10439..11236)
                     /locus_tag="VAH76_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO1386583.1"
                     /translation="MPSTYILNTNDKARERLCLQHQLYAKSSLDLLSEVGPSAAKTAL
                     EIGCGSGDMTLELAKLIGREGSLLTIDLSQDQLDHTQQRTRTYSNIRFKVWDVNHLSD
                     LGEKFDLIYCRMVLHHVADARSVILQMKNCLNSGGFVVCEEPSLFDSTFCSPPSPSYD
                     QFTQWARSCFSKNNRDFQIAHRLEQEFSSCGFEIEHCSLFQPLLRTNKEKLIYSMALD
                     DLTPQLLELGIAKREEIKQLHNELEDLAKTNSTMCWIRMHKIIAKLN"
     gene            complement(11266..12210)
                     /locus_tag="VAH76_001975"
     CDS             complement(11266..12210)
                     /locus_tag="VAH76_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO1386584.1"
                     /translation="MKPFVYLTNQFLEPIIPMLIPDWNIIHGWKMASGVDQSRVVALA
                     TTVWDQIDHSFLMQFPNLKIISHLGIGTDNIDINFLKQNHIILHSQPNAGVHDTAELA
                     IALLLALSRRVILNDRYTRNNEWVEKKPRFLGNHLLGKQLGLVGFGQIGEKIAQFAEP
                     FGLKIAYTARSQKNSPYLYCPTAANLASISDFLIICCSGGIDTQHLINKQVLDNLGTE
                     GYLINVARGSIVDQNALIDALQRHTIAGAALDVYQYEPEVPLALRQLDNVVLSPHMGS
                     STKENLNQMFQLQAKHLNQYLNEPLWQRESHLTLFEEQ"
     gene            12447..13469
                     /locus_tag="VAH76_001976"
     CDS             12447..13469
                     /locus_tag="VAH76_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HEO1386585.1"
                     /translation="MPVIPVEEFDGFILKLGKNNYHFCKNETPFNNSCSANIAIDKYC
                     TSKLLEKAGIPVPKAISLNCSEFQRNLHKDKIAQLNFPLVIKPIDGSLGIGVLCNIKT
                     WEELESHLTKYFSSYQCLIIEEFHGKLNSYRVLVFNNRILGIVQRYPAAVTGDRVHPI
                     HELIKQENLNRKRINEALGEITLDEEARIKLQELGITENYIPSFGEQIVLCYTSNATR
                     GGSYVTINKKMCKQNRKLLLQAAKILDLQLVGIDVECSDIINVPIEQSKGVILEVNHK
                     PSIRIHEFPMKGTPQKVTKKIMRSFITRHPLSYLYSIYNNQPTAFYVRSSILIIIMGL
                     ILLAVW"
     gene            complement(13531..>14624)
                     /locus_tag="VAH76_001977"
     CDS             complement(13531..>14624)
                     /locus_tag="VAH76_001977"
                     /inference="COORDINATES: protein motif:HMM:NF013253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEO1386586.1"
                     /translation="LSSLARRYLYYRAENGELANSTRKYLRSIKLLPRVMTSLSDIDS
                     TIELFEHKLSIPVFLAPMAFHCLFNQEGEVASAGACAQEGIGFCYNYCYSSRSIDSLK
                     KHVPEGVKFLHIYPWRERENFLAPIIRQAEESHLFSAILFTADHPHDRVQQFITPYFC
                     QEAHKYKHPEEVCTLPNLSLLGGKEGTCDRSLGWDFVDWLRQQTNLPIIIKGILSPKD
                     AELAIDHGVNGIVVSNHGGRQLDCTVPSLMVLPKIAKIVSGKNIKLFVDSGIEYSTDI
                     FKAIALGADAVLIGRPMLKALAQGQTGLSQYIARLKYDFQCDLASMGCSSLKMIGQHC
                     LYQVNSPELVKFSDQMPEQSVFRNKFIIK"
BASE COUNT         4650 a         2993 c         2801 g         4180 t
ORIGIN      
        1 tggtcattat ttcagcgatc cttggcatat ttataaatcg ctaaacctct tatataaaaa
       61 ttttttacgg tttataagca cacaccaaac tgaacaaatg ttgaccaaaa ctaatacttc
      121 atcaactaat attatacatt atttgtaggg ttctatagaa gccaattgcc gcttaacaga
      181 caacaaggct ccaagccacc cgagtataat agcaaataac acaagtagca agatttgccg
      241 tagtgacaaa caagctaaag gatagtgcat ttgatagaca ttagccagct gattcactgc
      301 gacgcctagg gttagaataa aaatattaac cagaaagatg gctaaaagcg cgccagctgc
      361 cccataccaa actccagaat ataaaaaagg acgaataatg taaggatctg ttgcgccaat
      421 cagttttaaa attttaattt cttcttgtct attatgaatg tctagacgta aggtattgcc
      481 aataataaat actacagcca aagctaataa cgccattaaa gcatttgctg ccttgccaga
      541 aaaacccaag atagcgtgca atcgattaat ccactccatg tccagtttcg ccaacgcgac
      601 ttgtggaaaa gcctgaagtt ttcgagataa taaatctaat tttgcaggag aatctataac
      661 taatgcggga actacttcaa tcactgcggg caaagggttt tcaggaagat accgcataat
      721 gtcttgcatg ccttcttgtt gagttaactc ggataatcca tctgctgcag atttgatgtt
      781 tgcctgacca actccttcag tagtgcgtac tttttcaagt aacgatgttt gctccgcttc
      841 cggcaatgaa gatttcaaaa acagggaaat gtgcccccct cgctgccaac gattggttag
      901 ttcacttaaa ttgtcagtga atacccaaaa aagtgtaggt aacgccaaag cgatggcaat
      961 aacaatgacg gtcatcatgg ttgctatcgg tttgcgacag aaaagattca agctattggc
     1021 cgcggcttgc aaatgatagg caagaagtga ttgtgatcgt tttaacacag ccttcctcct
     1081 ttcagcatga cgatgcgatg tttcattccg gctattaaag ccaaatcatg ggttgcaatt
     1141 aatatactaa ctccaacctg attaaattgt tcaaaaatct tcattatctc ggaagaaagt
     1201 tttggatcca ggttaccggt aggttcatct gccaataaca atgcaggctt atgaactaca
     1261 gcacgagcaa taccgactcg ttgctgttca ccaacagaca ggtgaactgg taacattttc
     1321 tctttactca ataatcccac catatccaaa gcagcatgca cccttttggc aatcatagga
     1381 taagcaattc cttgaatttg caagggcaag gcaacattat caaaaacagt tctgtcattc
     1441 agtagctgag gagattgaaa tgtaatgccc aactgactgc ggtgagcagc aacatctcgt
     1501 tttttgagat gatttaattt caaaccattg actgttaatt gaccagacgt cggccactca
     1561 agaagagcaa tcaacttgag caaggtgctt ttgccagcac ctgaatggcc agtaagaaag
     1621 accatttctc ctttttgcaa agaaaaatta acttggctta atgcctcaaa gccccccgga
     1681 taacgcttac taacctggtc aaatgtgatc atcatcgaat attgcactaa caaattgcgc
     1741 tgcgtcaaag ggcctcaaat cttcaatgcc ctcccctacc cctaaataac gaaaaggtat
     1801 ccccaaatca ttcgctatag caaataatat cccgcctttg gctgtcccat caagcttggt
     1861 catagtaatt ccagttaatt gtactgcttc atgaaactgc tttgcctgag ccaaggcatt
     1921 ctgcccaata ctcgcatcca ataccaacat ggtttcatga ggtgccttgg gatcgagttt
     1981 ctgcatgact cttttcactt ttttcaactc ttccattaaa ttgctttgag tatgcaaacg
     2041 cccggctgta tcagcgatta aaatatctat ttttctcgct ttggcagcct gaagagcatc
     2101 aaaaattact gaggcactgt cagcgccggt atgctgggca ataacaggta tgccatttct
     2161 ttcgccccat acatgcaatt gctccaccgc agcagcccga aacgtatcgc ccgcagctaa
     2221 catgactttt tttccctgct tttggaattg tttggctagt ttaccaatcg tggttgtttt
     2281 accggctcca ttgacaccaa ccattaatat gacaaaggga gaaccgtctt cagtttctat
     2341 ggaaagcggt ttctcttcct ggctcaaaat cgtttgcaaa tttgtcttta gagtctgata
     2401 aactgcttca ccattagaca gttgctttct tgccaagcct tcatttagct gtttcaatac
     2461 gtcttgagtg gtgtccaaac ctaaatcagc actaattaat aaggtctcca gctcttcgag
     2521 taactcctgg ctaatttctt ttttacctaa cagcagccga cttatgcctt cccccaattg
     2581 atgacgggtt ttgctaaggc ctttcctaaa ccgggcaaaa atactttctt tgggcgaagg
     2641 ctctttgaca gtcagttcct tgggctcttc aagcctggat tccatgctgc tttcggcatg
     2701 atcttgttgc tcttcggtat ccaaatttgg ttcggaattt tgatttcgtt taaaccattt
     2761 aatcatttta gtgtacaatt ccttaaaaat atgaaattct atcatctttt tagctttgag
     2821 gtgacattgt gcacaaatta ctcatttctc ttatgatgct actctcattt caggctttta
     2881 gccaagtcca agagtatacg ttaaacaatg gtttaaaagt actggtaaaa gaagatcacc
     2941 gagctccagt cgtagtatcc atgatttggt ataacgtggg ttcagcagat gagcctgtag
     3001 gcatcactgg tgtttctcat gccatagaac acatgatgtt taaaggaact tccaaatacc
     3061 cggtaggggt attttccaaa acaattgctg cattgggagg tcaggaaaat gcttttacaa
     3121 acaatgatta taccgcctat tatgaaaaat tagatgctgg tcatctggct acaagcttcg
     3181 aattggaagc cgatcgcatg aataatttac tgcttaatag cgaggaattc gctaaagaaa
     3241 ttaaagtcat tcaagaggaa agacgtttac gtactgacaa taacccgcag gcacttgctt
     3301 tcgaacgttt tctggccaca gcgcatctta cagcaccata caatcacccg gtgataggat
     3361 ggatgaatga tttgaaacaa atgaaagtag aggatttaaa aaaatggtat gaaagttatt
     3421 acgcaccaaa taatgccacc ttggtagtag taggcgatgt gaatcctgag aaagtacatg
     3481 ccttggctga acgttatttc ggctcaatag ccaaacgtcc catagcgtct cgtaaacccc
     3541 agcaagaacc ctcagcgctc gggaaaaaaa tggtttacat taacgcgcca gcgaaactgc
     3601 cattactgtt aattggctat actgtaccta gtgtaaaaac tgctaaaaat aactgggaac
     3661 cttacgcctt ggaaattatc gccgggatac ttgatgccgg ggaaagtgcg cgctttgcca
     3721 aacacctggt gcgtggcaat caagtagcca ctggtgcaga agcctattat aacctctatt
     3781 caaggtacca aagccaattt attgtctatg gggcgcctag ccaagatcat caaataaaag
     3841 atctggaaaa ggcattaatt acggaattgg aagccttaaa aaaagcgcct gtaagcaatc
     3901 aggaattaca aagggttaaa aatcaaatca tagcacaaaa aacttttgaa aaagattcta
     3961 tttttggtca ggccatggaa ctgggattgt tagaaacaat aggtctgggt tggaaaaata
     4021 cggaaacata caccaaagcc atcaatgaaa taactcccga gcaaattcag caagttgcgc
     4081 aacgatattt tcaagaaaat aacatgactg ttgcagagtt aaagccaatc aggcaagaag
     4141 aggtaagacc atgagtgttt taagagcttt tatcatttcg tttagtgtaa ttttttctca
     4201 caatctattc gccaattcat tcaaaactga aaaatggcag actaaaaatg gggtgcgagt
     4261 tgtattttat caagcaatgg aagtacctat gcttgatatt agtttggcat ttgctgccgg
     4321 ctctgcctat gacggtaagt attttggttt aagcgcatta acaaccaatt tgattaatca
     4381 aggcaattcc ggtaaagacg caacgactat tgcggaagct ttagcagaca ccggagcaca
     4441 atttaatgcc gaaacgagta gggatatggt tgtccttagt ctaagaactc ttactagcaa
     4501 agaggctttg cagcaatcta ccaaaacctt tagccaaatt attagtcatc ctgattttcc
     4561 taaagaagcc ttcgcaagag aaaaagatca acttctcatg gcagttgaac aaacagaaga
     4621 atcgccagat gatgtagcca tccagaactt ttttaaaacc ttatatcaag agcatcctta
     4681 tgcccatcct gtgcatggga cagttgagtc actaaatgcc attaaagaaa atcaagtcat
     4741 tgatttttat aaaaaatatt ttgttgctaa aaatggtata ttagttatgg ttggtgctat
     4801 tgacagttct caagctcatc aattggcaga acaattgacc caggatctac ctgcaggaga
     4861 gccagcacca acaattccca aggcctctca gctggccgat gcggaaaaaa ttaatgtacc
     4921 gtttccttcc tcacaaacag tagttcgctt gggacagata ggaatagatc atcataatca
     4981 aaattatttc cctttaatgg ttgggaatta tattttaggt ggaggaacat tagtttccag
     5041 gctaggtact gaggtaagag aaaaaagagg cttaacttac ggaatcgata gtcaatttgt
     5101 ccctatgcta ggtgaaggac cgtttatcat tagtttatca accaaaaact ccgaggccag
     5161 aaacgcactg cacattaccc aggataccct gataaaattt atcaaaaatg ggcctaacaa
     5221 ggaggagctg acttcagcga aacaatatct gacaggtagt tttcctctct cgctaggaag
     5281 caataccaac attgcaaacc tgttattgag aatggcgttc taccatttac ctgataatta
     5341 tctggatacc tatgtcgcta aaatcaacgc ggtaaccgat gctgaaatca ggcaagcatt
     5401 ccagcaacag gtgaaccctg aaaaattact cctggtaacg gttggacaat cttgaaacag
     5461 gtaatacgga ttatcggagg attatatcgg gggaaaaaaa tacatttccc cgatgtagat
     5521 ggcctgagac caacgccaga cagagttcgg gaaacactct ttaattggct aatgaatgat
     5581 attagaaatg cccgctgtct ggatgctttt gcaggtagtg gcgcgttggg atttgaagcc
     5641 tattccagag gagcgtctca tgtcgttttt atcgaacaat ctcccaaagc tcatgccagt
     5701 ttgcaaaata ccattcgtca atttgatacc cccaacttgc accttataaa aactgatacg
     5761 ttaaattatc ttaaacaaag caccgagcaa ttcgatctga tttttttaga tcctccctac
     5821 gctctcaact atgttcctca atgtcttgat catatcataa caaataacct tcttgttaaa
     5881 ggaggtcttg tctacattga atcatcatct cctattgaag taaaaacaga actttggaag
     5941 caattgaaat taaaacaagc cggtcaagtc atttatggat tatttgaaaa actctaacaa
     6001 acaatgtctt gacaacaagc cttcctcctg tttcaatcaa actaatattt ccaaatggta
     6061 aagaacattg aatgaacaac aataagattg tcataatgtt tattttttca gcgcttcttg
     6121 ctggttgcgc tggaacaatg aaatttaaaa aaccacccat caataatcca agtgatgatg
     6181 cgactattaa attagcagaa gctgccgttt cagtcagtga ctccatgctt gaaatggcaa
     6241 aagttgaaaa agtaattact ccaccaagca aagacaatac tttaactata cctaatgctt
     6301 ataacttgca agcaagagcc agtgtcgatt ggtctggtcc gattgaagaa ttgactgcgc
     6361 gtatcgccaa agcagcacat ttcaggtttc gtgttttagg caagtcgcct tcagttccag
     6421 ttttaataag catcagtacc aaagatgaga gtcttgctga gatccttaga gatattgatt
     6481 atcaagctgg gataaaagcc agtattcatg tgtatcctaa cagtcaggtc gtagaattgc
     6541 gctatgcgaa aatttattct tagtttatct attttgctat cagccctcct ggtagcctgt
     6601 tcttctcgta atcattacgg ggatactggc tcattggcag gccttcaagc gatggctgat
     6661 tcaaaataca cccgagctca aaaaaaacaa aaaatgggta aaattcgcga gatggccctt
     6721 aaggaaaccg ctttaagtgt tggcgctcag gctgggttag cctggagagc aaaaataatt
     6781 gacgagcaat taaacaaaca ggcaaggaat cttgatgcaa tttatgattt taattccctg
     6841 gtcttagagc ataatatttt acctcctgta ttacttgagg gaaggaatac tttgaatctt
     6901 gcagatgctc aaagtatccg tgtttcagat cgcacctata aagtcgcaaa acaagctcat
     6961 ttcattacca ctccacctac ctggcggcaa tatttgtgga tggattatgt gaaacctgaa
     7021 gctcccaatg tcactctact ccccaaaacc aaggctgaaa aagaaatatg gtgcatctat
     7081 actgaaagag gatggaaaaa tggtatagat caggcaaaca ccattttgga agaaaacatt
     7141 gcccgcatta aagaagattt cggcggtatg attctttata gaaaattact ggctatgaac
     7201 atggtatctc ctccttatgt ttctcatact gacttgggcg taactgggga tggctcagaa
     7261 attcatatcg atgacagggt cttacgcatc acggcgttac ctgaacttaa tgtcaatagt
     7321 gcggaatgga gagctgcggt cgccaaagat gaaaatgctc tcgaacgttt caaaaatatg
     7381 gaaaaactgg caaatcaggc taaaattgtt ataacgaaca aatcctggca acctattata
     7441 gcaccagtaa gttaatgtct atggataata ttaatttaat gcccgatgag ccaactcgtt
     7501 tcactccagt attcatggac agaatgctgg agcatgcaga aagcctgaat gcttcagata
     7561 taacaatcca aactggcgaa cccatttttg cagaggttta tggaagactt cttaaaatta
     7621 caaatcgacg tctttccaat acagaattgg gcgaccttat taattccatt tatggcccga
     7681 atgccacgac acaattactt tccggtaaag acatagatac tcattatgag tttcgaccta
     7741 accgtggtgt acgctatcga tatagggtta acgcgactgc ctgcctggtt gaaggtcatg
     7801 atgccattca gatcacatta agaactattc caaccacacc acccaaactc agtaccatga
     7861 acctccctga taacattatt gaagcgattg ctccacagga aggaattgtg tttatcacag
     7921 gcgctaccgg ttcgggcaaa tcgacattgt tggcttccat tattcgagag cttattgaaa
     7981 catcagactc aaaccgcaaa gtattaactt acgaatcccc gatagaattc gtctatgatg
     8041 aaattgaaac aatttccgct gtagtaagcc aatcagagat tccaagacac ttacccaact
     8101 ttgctgatgg agtaagaaac gcattaagac gaaagccgag attaattatg gtgggtgaat
     8161 gtcgtgatgc agaaaccatc agcgccgctc ttgaagccgc tttaacagga cacccggtct
     8221 acacaaccct gcatacttct ggtgttgcag aaacgatgcg acgtctggtc acatctttta
     8281 gcggggaaga gcgtttggga agaaccatcg atattcttga aacaattagg ctttgcattt
     8341 ggcaaaaact ggtcccaacc gttgatgaaa gacgtgtagc cttaagggaa tatctggtat
     8401 ttgatgagga agtgagagat attctattgg agggagaccc taacgaagtg acttcagcca
     8461 cacgtaagtt agtgaggcaa aaagggcaat taatgacttg ggacgcaaaa atgaaatttg
     8521 aacaggggat tatcagtgag cgagtttata aattaatcat tgctggggcc aaagaatatc
     8581 aacaataatc aaaattaatc tgcttgcact tgccatagaa aatcattgat ataccgcata
     8641 taaaacaatc aagatcaagg attcgattca tgatatttca caggaaatgc ctatcccatt
     8701 tttttaattt atttatttcg ttaattttag gctcctctct caattgggct tccgtcaatc
     8761 agcacagcac agattcgcca acactcaaca ttaaaatttt aggcattaat gattttcatg
     8821 gtcaaataag cagtggaaga actgtaaaaa acgaacctgt tgggggggca gctatcctgg
     8881 ccgcttatat taaagaagcc caattgggaa tggaagatcg atcaataatt accatcatgg
     8941 gtgatttagt tggagcctcc cccccttctt caggactgtt gaatgatgag cctagtattt
     9001 tattcattaa tagcttggga aataatcaat gcaagcccga atcaagaatg aatcctcttt
     9061 gcaacatagt tgccacggta ggaaaccatg agcttgataa agggcaaaaa gccatgttcg
     9121 atttaattta tggtaccaat aatccaccga ctgattcatg gatctcgtta cccagttatc
     9181 cgggctcatc ttatccctat gtttctgcga atatcgttga cgctaagtca gaacaacctc
     9241 tcttcccgcc ctacataatt aaacggatac ataatattcc catagcattt attggggctg
     9301 ttttaaaaga tgcggcggac tccatgtttc ctgcaaatgc agaaggaatt aaatttttag
     9361 atgaagcaga cagtattaat cgttacatcc cggaaattaa agcgaaagga gtcaatgtta
     9421 ttattgtcct gattcatgaa ggtgggaatc aaattcctta tgagggagat acccaaactg
     9481 cctcagcagt tgaaggccat attaaagaaa tagtctattc ccttggtgat gaaatcgacg
     9541 ttgttatggg tggccacacc catcaatttc taaatgcttt ccttcgtaat cgcaatgggc
     9601 acgagatcct ggtcacacaa gcgaatagtt acagtgctgc atttgctgaa gttaccctcc
     9661 atatcgatac cagaaatcat gcggtaaaaa acaaatcagc caaaattatt acaacttatg
     9721 ctaatcgatg gccaggaaca aatccggata cacaagcatt ggaaattgtt aaattagcag
     9781 aagataaaat tgggccaata ataaatagcc atgtaggcac cgcagaatat gatctgctaa
     9841 gaaaacaaaa tgaagatgga gagtcgagtt taggaaatct gatcgcagat gcttatagaa
     9901 ctgtaatgaa taccgatctg ggcatcacta atcccaatgg aattcgtgat gatttaaaag
     9961 caggtgaagt taactgggga aatatctatt ctattttacc cttttccaac aggatagtca
    10021 taattacctt aactggaaat gatatttatg acttgttaga gcaacaatgg atgggctctt
    10081 atatcaatat gttacaaatt tccgggtttg cctacaccta tgattcacat caacctctgg
    10141 gacataaaat aattgaaatt agacatcaaa acaaaccatt gcttagagaa aagatgtata
    10201 ccatagccac ttcagatttt cttgccagcg ggaatggagt tttttcagtt atgaaaagag
    10261 gaaaaataat ccatattggt caaagtgatc atgacacggt aatcgcttat attaaaagtt
    10321 tacctcaacc ctttcacgcg tccattgagg caaggataaa agaatcagag actggattaa
    10381 aaacaggagt tggatagact aaatttgttt taccgtcagc ccctgttttt attatttatt
    10441 aattaagttt tgctattatt ttatgcattc taatccagca catagtagaa tttgttttcg
    10501 ctaagtcctc aagttcatta tgtaattgtt tgatttcctc ccttttagct atgccaagct
    10561 ctaaaagctg aggggtcaaa tcatcaagcg ccattgaata aattagtttt tctttgttcg
    10621 ttctgagcaa gggttgaaat aaagaacaat gctcaatttc aaaaccgcag gatgaaaact
    10681 cttgctctaa tcgatgcgct atctgaaaat cccgattgtt tttgctaaag catgacctcg
    10741 cccattgtgt aaactggtca taggagggtg aaggaggaga gcaaaaggta gaatcaaata
    10801 aagagggttc ttcacatacg acgaaaccac cagaattaag acaatttttc atttgaagaa
    10861 tgacagagcg cgcatcagcc acatgatgta aaaccattcg acaataaatc aaatcaaatt
    10921 tttccccaag atctgaaaga tgattaacat cccacacctt aaatcgtata ttgctatagg
    10981 ttctggttcg ttgttgagta tgatccagtt gatcctgact caaatctata gtaagcaaac
    11041 tgccctcccg gccaattaat ttggctaatt cgagggtcat atcaccagag ccacatccta
    11101 tctccagagc agtctttgcc gctgaaggcc caacctcaga aagtaaatcc aggctgctct
    11161 tggcatacaa ttgatgttgc agacaaagac gctctctcgc cttgtcattg gtatttagaa
    11221 tgtaagtact gggcataata tatccttata tttgataaaa aataattatt gttcttcaaa
    11281 aagagttaga tggctttccc tttgccacaa aggctcattt aaatattggt ttaagtgttt
    11341 tgcctgtaat tgaaacatct ggtttaaatt ttctttagtt gagctaccca tatgtggtga
    11401 taagacgacg ttatcaagtt gccttaaagc gagaggaacc tccggttcat actggtatac
    11461 atccaaagcg gcaccagcga ttgtatgtcg ttgcaaagca tcaatcagag cgttttgatc
    11521 cactatactg cctcgcgcaa cattaatcaa atatccctct gtccctaaat tatctaaaac
    11581 ctgtttgtta attaaatgtt gtgtatcaat accaccggaa caacaaatga ttaaaaaatc
    11641 actaatagat gctaaatttg cggctgttgg acaatacaag taagggctat ttttttgact
    11701 tcgggccgta taagcaattt ttagcccgaa aggctctgca aattgcgcta tcttttcacc
    11761 gatttgaccg aatccaacca aacccaattg ctttcccaat aaatggttgc ccagaaaacg
    11821 gggctttttc tcaacccatt cattatttcg ggtatagcga tcatttagaa tcacccttcg
    11881 cgacagtgct aacaataaag cgatagcaag ttctgctgta tcatgaacac ctgcatttgg
    11941 ttgtgaatgc agaataatat gattttgctt taaaaaatta atatcaatat tgtccgttcc
    12001 tattcccagg tgactaataa ttttcaagtt agggaattgc attaaaaacg aatggtcaat
    12061 ctgatcccat accgttgtgg ctaaagccac aacccgggat tgatccactc ctgaagccat
    12121 tttccaacca tgaataatgt tccaatcagg aatcagcatg ggaataattg gctctagaaa
    12181 ttggttggtc aggtaaacaa atggcttcat taaaatgtcc tatttttcag aataaagatt
    12241 ccgtgtgttg tccatgatat tctttctcat ctttctgaca agcaaataaa aacaagcggt
    12301 aaaatgagca ttctaaagta gagacatata gcgattatta cggttatgat actatattct
    12361 tttactattg catttcatta ttccaaggag acgttaccat agagaggaat gcacagcaat
    12421 attttgaaag cgcgaaggaa ttgttcttgc cagtaatacc tgtagaggag tttgatggtt
    12481 ttatacttaa attgggcaag aataactacc atttttgcaa aaatgaaact ccttttaata
    12541 atagctgtag tgccaatatt gctattgata aatattgtac ctctaaacta ttggaaaaag
    12601 ctggtattcc tgttcccaaa gctatctctt taaactgtag tgaatttcaa cggaatttgc
    12661 ataaagataa aattgcacaa ttaaactttc ccttagtcat taaaccaata gacggttcgc
    12721 tgggaatcgg cgttttatgc aacataaaaa catgggaaga actggaaagc catttaacaa
    12781 aatatttttc ctcttatcag tgcttaataa tagaagaatt ccacggcaaa ctgaattctt
    12841 accgtgtatt ggtttttaat aaccgtatcc ttggtatagt tcaacgttat ccagccgctg
    12901 taactggcga tagagtacat cccatacatg agttaattaa acaggaaaat ttaaacagaa
    12961 aaagaatcaa tgaagcttta ggtgaaatta ctttagatga ggaagctcgg attaaacttc
    13021 aagagctggg cataacagaa aattacatcc cctcttttgg agaacaaatt gttttatgct
    13081 atacaagcaa tgcaacaaga ggtgggtcct atgtcaccat aaacaagaag atgtgcaagc
    13141 aaaatcgcaa attattgctt caagccgcca aaatactcga cttgcaatta gttgggatag
    13201 atgtggaatg ctcagatatt atcaatgttc ctatagaaca atcaaaaggg gtcatactgg
    13261 aagttaacca caaacccagc atccgaattc atgagtttcc aatgaaagga accccacaaa
    13321 aagtcacaaa aaaaattatg cgcagtttca tcacccgcca tccgttgtct tacttatatt
    13381 ctatctacaa caaccagccg accgcttttt atgtccgcag ttctattctt attatcatta
    13441 tgggattaat tcttttagcc gtgtggtaat gcaccggacc taaaactaat gtcatttcct
    13501 aatctttact tccaatacat agcagagagt ttatttgatt ataaatttat ttctgaaaac
    13561 actttgctca ggcatctgat cggagaactt cacaagttcg ggtgaattga cttgatacaa
    13621 acaatgctga ccaatcattt tgagactgct acatcccatg ctggccagat cacactgaaa
    13681 atcatatttt aatcgggcaa tatactgact taatccagtt tgaccttgcg ccaaagcctt
    13741 taacataggt ctgcctatga gtacagcatc agcacccaaa gcgatagctt taaaaatatc
    13801 cgtgctgtat tcaatcccgg aatcgacaaa tagctttata ttttttccag atacaatttt
    13861 tgcaattttg ggtaaaacca ttaatgatgg aacagtacaa tccaattgcc gccctccatg
    13921 attactcact acgatgccat tgacaccatg atcaattgca agctctgcgt ctttaggact
    13981 aagtattcct ttaataatga taggcaagtt agtttgttgt ctcagccaat ccacgaaatc
    14041 ccaacctaat gaacgatcac aagttccttc tttgccacca agcaaactga gatttggaag
    14101 agtgcaaact tcctccgggt gtttgtattt atgggcctct tggcaaaaat agggagtaat
    14161 aaactgttga acacgatcat gaggatgatc ggcagtaaac aaaatggctg aaaacaaatg
    14221 actttcctct gcttgccgta taatgggagc taaaaaattt tctctttccc gccatgggta
    14281 gatatgcaag aatttcaccc cctctggcac atgctttttt aaactgtcta tcgagcggga
    14341 tgaataacaa taattataac aaaaaccgat accctcttga gcacacgcac cagcgctcgc
    14401 tacttcacct tcctgattaa acaaacaatg aaaagccatc ggtgcaagaa acactggaat
    14461 ggaaagtttg tgttcaaaaa gttcaatagt gctatcaata tctgaaagac ttgtcataac
    14521 ccgtggtaat agctttattg agcgcaaata tttgcgggta gaattggcaa gttcgccatt
    14581 ttcagctcta tagtataaat atctgcgggc taaagatgaa agct
//
LOCUS       DATNSN010000086        14038 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.086, whole genome shotgun sequence.
ACCESSION   DATNSN010000086 DATNSN010000000
VERSION     DATNSN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.086"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            177..1316
                     /locus_tag="VAH76_001978"
     CDS             177..1316
                     /locus_tag="VAH76_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEO1386587.1"
                     /translation="MFRVLIFLSCLVILTPNTIADTNPCEKHSCIAVIDAGSTGSRLH
                     IYSYDMDDTNTPIHIEEIWNKKIKPGFASIQPNSVTIDAYLTMLLADAPIHNIPVYFY
                     ATAGMRLLPQSQQKKYYDELDSWFRQQSQWQLVEAKTITGNDEALFDWLAVNYKLDTL
                     KSVQNKSVGVMDMGGASVQIVFPMPKNAEISKHNQVELNIYGQNINLYVHSFLGLGQT
                     EMSHQFLNSPSCFANDYPLPDGESGQGNAPSCKEEVTSLMNSVHKVNQQIQPLLALNP
                     VNEWYSIGGISNLASSQLFHFENSELTNQSLLQQGDNQICHQQWDILNGQYPDDEYLY
                     QYCLLSSYYYALMVDGYGINPNQTIHYIPPEQNLDWTIGVVLHRA"
     gene            complement(1321..1854)
                     /locus_tag="VAH76_001979"
     CDS             complement(1321..1854)
                     /locus_tag="VAH76_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HEO1386588.1"
                     /translation="MHHVEDLISIFNYCFENDYNTRLVRGGDEPIYLPADDQRPYHAL
                     FFAHGFFSSALHECAHWLIAGEERRKLVDFGYWYAPDGRNEEQQTLFQRVEVKPQALE
                     WILSMAAGHKFRVSIDNLNGAEVDTEAFKQSIYKQVFHYCTQGLPQRALHFRNALCAF
                     YRQPSPLRIDDFSIDEL"
     gene            complement(1865..3670)
                     /locus_tag="VAH76_001980"
     CDS             complement(1865..3670)
                     /locus_tag="VAH76_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1907 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386589.1"
                     /translation="MVRQCTSFKGGLNLNFIENAEFESAKDLPLEEQRAVLIRNTLRL
                     LMMGWNTSWTELFSWETFKAIYLQRDPLLLKEFRLAFQKGFDAIFTQLNGRQFNSQQN
                     EQIQLYLSNCLSYLPLFDLNPYESIKVPQRINDQWVMVDYYIKPIELTNHRGKNDLIL
                     RDDDRVFAYGLEPIDNPNAQPHLIFMGTTYPAGQGFISQLNTDLKGFETVGYSLYKNG
                     RDRIKDWILKQNSKIHVCGISLGGSLSLLLAIDMGKYLSRVDALNPAGLHDTDNKVKY
                     DKWDKIKKKPLVVVQQQGNDPVSFFGVWKKEWNILHVKPPKDKQGPNLFCDHFLNYAG
                     FAETEFTYVSAEQQNAKRKARNFWLYSLGRGVLYYFGLAPYTYLIRPVIHYIAEQWPL
                     IVFGIIPLLTIGTLIGLSTAGIIPAIVSIGTLAAFAISSGIFYLSFGLSSNEEDKNTW
                     AGMFDFILKGNEKVPDENEIKPAKLHHPSTPRNPEMDIYKNETAIDLTYKQINTYYQV
                     TRCLLKQKEFLPLKDKPEKRFGGLSKKEALLASMNPASHDEVISLQIPKAKAVHIKHS
                     LTLAAKIGMENEIELKSALKKEYNQYQIGKHQRFC"
     gene            complement(3995..4600)
                     /gene="gstA"
                     /locus_tag="VAH76_001981"
     CDS             complement(3995..4600)
                     /gene="gstA"
                     /locus_tag="VAH76_001981"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEO1386590.1"
                     /translation="MKLYYCRGACSLVVRIILNELGLNFRDEEVDLKAKKTAGGDDFF
                     KINPKGAVPTLVLDNGDVLTENQVILQYLADTTPGQKLLAAVGESKRYHTLEWLNYIS
                     TELHKSLGMFFNPTVSEEIKSKILTPLVMTRFAYTNTRLSKGSYLMGNEFSLPDAYLF
                     VMVSWAKYFKLDLSQFEHLTKFMDLMLARPSVAKSLQQEKL"
     gene            complement(4797..5792)
                     /locus_tag="VAH76_001982"
     CDS             complement(4797..5792)
                     /locus_tag="VAH76_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386591.1"
                     /translation="MAICINKGTDHFFISIGKINQHSFIMLGVYDDFQVSHLLCRVGK
                     IFDLPNQTKGIKRCLSIYSALGGAIFASSKAKIEDEGITRKRKGSVPISYQAYDISYE
                     QYCEFVHYLESIQTESNQFECFKPFVQNGNAVYFSQTSSRVFATGSHWKELNEEIHEI
                     NTGNTCRHSAIKLIEAVTKTSVPSSISSCFFINLPYKTQLDYGKPSQNIPFYVLPPPP
                     PSIHPGFNKEKCLIAKKLYHRIEQLPVLEPNSPMTKRKFNSLKNLYLQIIGSQKNQSI
                     DELLFGIQQWKEKNRVDLQTLRRTYFWDSFIFRESATMKLINEIEKDLKCAKCPY"
     gene            complement(5833..7095)
                     /locus_tag="VAH76_001983"
     CDS             complement(5833..7095)
                     /locus_tag="VAH76_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO1386592.1"
                     /translation="MKINNLVSFLLAFCLASLSYNISAAESPVVSHHKIQKEMSKYFK
                     VYHNKEKFTATAVSVFIPQEKNNDALDVATFVKGTMGYPPLSQEITPLNLFDIGSITK
                     SFTSLILLQLQTEGKLSLDDPLGKWLPQYKEWKDVKLRQLLNMTSGIPNYSEDPEFAK
                     KMEDNFAAIWGDEELLGYAHPEKPIETDRANRYEYSNSNYILAGMVIEKVTQDTFENQ
                     LKQRIINTNNGLNNSFYPAGPHGPEVEKSIRDRRVHGYFYDEDNKKLIDTISNDLSWA
                     GAAGAVIANTEDVGRWVQLLYHGLLIEPVFRDEALSELESVVSIKTGLPIPSVTEDEP
                     YGFGLGVGFYYDKNSRQRFWTYQGSTLGYRVMYLWNPCNNVTVVVALNGKGGSGNEKS
                     KMGNHIHELCLNIHKVIVKNHPHLQCAS"
     gene            complement(7342..8754)
                     /gene="gltX"
                     /locus_tag="VAH76_001984"
     CDS             complement(7342..8754)
                     /gene="gltX"
                     /locus_tag="VAH76_001984"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO1386593.1"
                     /translation="MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDT
                     DRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLKEGKAYRCQCSK
                     ERLEALREAQLAAKEKPRYDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIH
                     VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDA
                     PVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
                     SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLN
                     QGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHE
                     RLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKN
                     KSIKRLEDALEKIRARASAV"
     gene            8853..11585
                     /locus_tag="VAH76_001985"
     CDS             8853..11585
                     /locus_tag="VAH76_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386594.1"
                     /translation="MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSR
                     LGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINPEVKALAFYNADGRLIAYRGGKH
                     SIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKELSTPKIFQADD
                     ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSM
                     KQILSNEFETEIRVSSPGELGIIEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLEL
                     LEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFTNVLLESKLDPLQLD
                     YVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDIRGCIDEVLSLASPNAHKKG
                     IDLIPITDINVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTL
                     LFAITDTGIGISPEDQTKLFTAFNQADTSITRRYGGSGLGLVICKKLCEEMHGRISLT
                     SEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAIGNGLGFWGI
                     EAIRVDSFNKLSRTLTKHKDCKIAFINVNQGCERQAAELIAKHEQIPFVLISKWPIND
                     FAALGARGFLYKPISIQKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAE
                     DNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFDMILLDLHMPKLNGLEAAKMI
                     RQKSLMNKPSPIVIITASSSDLSSIDMKKSGVDFCFQKPIDEKQLLIQILRIVDKTKH
                     AAIDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIELMHQKNVKGLADLAHKLHG
                     ACCFCGVPILQKRVAQLEKLARRTANADNLTEAFTDLIQSIDAVISEYENQYSQ"
     gene            11683..12927
                     /locus_tag="VAH76_001986"
     CDS             11683..12927
                     /locus_tag="VAH76_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO1386595.1"
                     /translation="MSVKNAIYAQSGGVTAVINASACGVIQTAKLFPEQIGKVYAAKN
                     GIIGALNEELIDTSLESEEDIAKLMQTPSGAFGSCRYKLKDNGEEYQRLIAVFKAHNI
                     GYFFYNGGGDSQDTAHKVSQLGKTMGYPITCIGIPKTVDNDLPFTDNCPGFGSVAKYI
                     AISTKEAGFDVASMAASSTKVFILEVMGRHAGWIAAASGLAEQNPGEPPHIILLPEVP
                     FEPIRFLKKVKDCVNSYGYCVIVVSEGIRNKEGKFLSEAGLRDAFGHAQLGGVAPVIA
                     QLIKSELGFKYHWAVADYLQRAARHIASQVDLDQAYALGKAAVEYAVKGYNAIMPIVI
                     REQDEPYQWSIGQVPLVEVANQEKAMPPEYIAEDGMGITDACKRYLSPLIQGEAYPNY
                     KNGLPDYVRLKNQLVAKKINQD"
     gene            complement(12924..13337)
                     /locus_tag="VAH76_001987"
     CDS             complement(12924..13337)
                     /locus_tag="VAH76_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEO1386596.1"
                     /translation="MRTKGFTLIELMIVAAILGILLAIAFPAYQDYTIRARVAEGLEK
                     ASFAKLAVAEKILIDNILPLNQADTSYSSPIPTDSVASIAIGNQGIITITYTANAGNG
                     TITMVPTIDATNRILTWDCTGGTLISKYRPAYCRP"
     gene            complement(13539..13952)
                     /locus_tag="VAH76_001988"
     CDS             complement(13539..13952)
                     /locus_tag="VAH76_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEO1386597.1"
                     /translation="MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVSEGLQM
                     ASSAKLAVSETAITNNALPTTQAETGYESPAPTANVTGIAIGANGVITITYTPAAGDG
                     TIIMTPTLDANNGDVTWNCTGGSLSPKYRPASCRP"
BASE COUNT         4469 a         2865 c         2502 g         4202 t
ORIGIN      
        1 ataatttaaa tttaaatcaa attgttaatt ttcaacccat aaaacagcga ctcttcatca
       61 ctcgacaaac caatatccca caatgttttg tacagtttat ttaaacacac tatattgaca
      121 acaaaatata cataatgtac cattcgaaaa cttcaatttt ggctctatat caactaatgt
      181 ttcgagtttt gatttttctt tcttgcctag taattttgac gccaaacact attgctgaca
      241 ctaacccctg tgaaaaacac tcttgtatag ctgtcattga tgccggaagt actggttcca
      301 ggctacatat ctactcctat gatatggatg acaccaatac tccaattcat attgaggaaa
      361 tttggaataa aaaaatcaaa cctggattcg caagcataca gcctaattct gtaacaattg
      421 atgcctacct gacaatgcta ttggctgatg caccaattca taatatacct gtttactttt
      481 acgcaacagc tggtatgcga ctactccccc aatcacaaca aaaaaaatac tatgatgaat
      541 tggactcctg gtttagacaa caatcgcaat ggcagttggt cgaggcaaaa accatcactg
      601 ggaacgacga agcattgttt gactggcttg ctgtaaatta taaacttgat acattaaaat
      661 cagtccaaaa taaatcagtt ggtgtcatgg atatgggcgg cgcttctgtt caaattgtct
      721 ttccaatgcc taaaaatgcg gagattagca aacataacca ggtagaactc aatatctatg
      781 ggcagaatat caatctgtat gttcacagtt tcctggggtt aggccaaaca gaaatgtcac
      841 atcaattcct taactccccc tcttgctttg caaacgacta tcctttgccg gatggggagt
      901 caggacaggg caatgctcca tcatgcaagg aagaagtaac ctcattaatg aatagtgtcc
      961 ataaggtaaa tcaacaaata caacctttgc ttgctttaaa tcccgtgaat gaatggtaca
     1021 gtattggtgg tatttccaat cttgccagca gccaattatt tcattttgaa aacagcgagc
     1081 ttactaatca aagtttgctg caacaagggg acaaccaaat ttgccatcag caatgggata
     1141 tacttaatgg ccagtatcct gacgatgagt acctctacca atattgtctt ttatcctctt
     1201 attactacgc attaatggtt gatggttatg gcattaaccc caatcaaacc atccattata
     1261 ttccgccaga acaaaatctc gattggacaa ttggagtcgt gcttcaccgc gcttgaagca
     1321 ttacaattca tcgattgaaa aatcatcaat tctcagggga gaaggttgac ggtaaaaagc
     1381 acataaagca ttacgaaaat gtagcgcacg ctgtggtaac ccctgggtac aataatgaaa
     1441 cacctgctta tagattgact gtttaaaggc ctccgtgtct acttcagccc catttaaatt
     1501 atcaatactt accctaaatt tatgtcccgc ggccatagaa agaatccact ctaaagcctg
     1561 tggcttcact tccaccctct gaaacagagt ttgttgttcc tcattgcgtc catcaggagc
     1621 ataccaatag ccaaaatcga ctagttttct tctttcttct ccagcaataa gccaatgagc
     1681 gcattcatgc aaagcactgc taaaaaatcc atgtgcgaaa aatagcgcat gataaggcct
     1741 ctggtcatct gcaggaagat aaataggctc atctccccct ctaaccagac gggtattgta
     1801 gtcattctca aagcaataat taaaaatact aattagatct tctacgtgat gcacgaatcg
     1861 ttacctagca aaacctttga tgcttaccta tctgatactg attatactct tttttcaaag
     1921 cagattttaa ttctatttca ttttccatac ctattttagc tgccaaagtt aaagaatgct
     1981 tgatatgcac agcctttgcc ttgggtatct gaagagaaat gacttcgtca tgcgaggcag
     2041 gattcatgct tgctagtaag gcttcttttt tagatagccc tccaaaacgt ttctcgggtt
     2101 tatccttaag aggaagaaac tctttttgtt tcagaagaca acgagttacc tgataatagg
     2161 tattaatttg cttataagtt aaatcgatcg cagtttcgtt cttatagatg tccatttcag
     2221 gatttctggg tgtagatgga tgatgaagtt tggccggttt tatctcattc tcatccggta
     2281 ccttttcgtt accttttaaa atgaaatcaa acatccctgc ccaagtgttt ttgtcttctt
     2341 cattactact taagccaaaa gacaaataaa aaattccact ggatattgca aaagcagcaa
     2401 gagtacctat tgaaacaata gcgggaataa ttcctgcggt acttaaccct ataagagtac
     2461 caatagtaag taaaggaata ataccgaata cgattagcgg ccattgctct gcaatatagt
     2521 gtattactgg tctgattaag taggtatagg gagccaggcc aaaataataa agaacccctc
     2581 tccctaaaga ataaagccag aaattccttg ctttcctttt ggcattttgt tgctcagcag
     2641 aaacataagt aaattctgtt tcagcaaatc cggcataatt cagaaaatga tcacaaaaaa
     2701 gattggggcc ttgtttatct tttggaggct tgacatgtaa aatgttccac tcttttttcc
     2761 aaactccaaa aaaagacacc ggatcgttac cttgctgttg gacaacaacc aaaggtttct
     2821 ttttgatttt atcccattta tcgtacttca ctttattatc agtatcatga agtcctgcag
     2881 ggtttaaggc atccactctg gaaaggtatt ttcccatatc aatagccaaa agcagactta
     2941 aagaacctcc aaggcttata ccacacacat gaattttact gttttgtttt aaaatccagt
     3001 ccttaattct atctcttcca tttttatata aagaatagcc aaccgtttca aatcctttca
     3061 aatcagtatt taattgggaa ataaagccct gacctgcagg ataagtggtt cccataaaaa
     3121 ttaaatgagg ctgggcattt ggattatcta ttggctcaag tccataagca aaaaccctat
     3181 catcatctcg caaaatcaga tcattttttc cacgatgatt tgtaagttct atgggcttga
     3241 tgtaatagtc aaccataacc cattgatcat tgattctctg gggaacttta atcgactcat
     3301 aaggatttaa atcaaataaa ggcaaataac taagacagtt actaaggtaa agttgtattt
     3361 gttcattttg ttgcgaatta aattgcctgc cgtttaattg ggtaaatatt gcatcaaaac
     3421 ccttctgaaa ggctaatcga aattctttca gcaacagcgg atctctttga aggtaaatag
     3481 ccttaaaggt ttcccaggaa aataattctg tccatgaggt attccaaccc atcattaaca
     3541 atcgcaaagt attacgaata agaacagccc tttgctcttc aagaggtaag tcttttgcag
     3601 attcaaactc agcattctca ataaaattta aattcaaacc acctttaaag cttgtacact
     3661 gcctaaccat aaaaacctct gaatagcttg tttctaacta tgagttcaga caattacaat
     3721 cactggctcg cggactgcta agcgaatcat ttaaaaaccc agtaactgta tattactatc
     3781 aatgaaaagt tcaaatttta gcacaataac aagaattatt gccaataact taaacccaaa
     3841 gggtttattt tgcccaaaaa gatgggctgt ctaacaagaa agttaaaccc ccatacttca
     3901 aggcattgga tataatgaat cttactcccc cttttaaaaa gtactatata gaagaagcaa
     3961 attgggttgc aatgaattaa taaagcaacc cgcgctataa cttttcttgc tgtagtgact
     4021 tggcaaccga agggcgagcc agcataagat ccataaattt tgtcaaatgc tcaaactgag
     4081 acaaatccag tttgaaatat tttgcccagc ttaccataac aaacagatac gcatcaggca
     4141 aactaaattc atttcccatc aaataagatc cttttgacaa acgagtattg gtataagcaa
     4201 aacgggtcat gaccaaaggt gtcaaaattt tggattttat ttcctctgaa acagtaggat
     4261 taaaaaacat tccaagcgac ttatgcagct ctgtagaaat ataatttaac cattccagag
     4321 tatgatatct tttactctct ccaacagcag ccaacaactt ttgtcctgga gtagtatctg
     4381 ccagatactg tagaataacc tgattttcag tcagaacatc gccgttatca agaaccaggg
     4441 ttgggaccgc tcctttagga ttaattttaa agaaatcatc gccgcctgct gtttttttgg
     4501 cttttaaatc gacctcttca tcccggaagt ttaatcctaa ctcattgagt ataatacgaa
     4561 ctactaatga acaagcacct ctgcaataat ataatttcat gttcactcct ttgatgatgt
     4621 ttaaataata atagctaagt tagcataaaa tgcggcaaaa aacttgaaat gtaaaaaacc
     4681 atacagtgaa aaaggatgat cttgtatgtg gtttatttaa tcagaccacg tttaatcagg
     4741 ctctataaga agcaaagatt cgatatgctc taaccctgat attatcataa caatatttaa
     4801 tatgggcact tggcgcattt gagatctttc tcaatttcat tgattaattt catagtagct
     4861 gattccctga aaataaaaga atcccaaaaa taagttcttc ttaatgtttg caaatcaact
     4921 cgattttttt ccttccattg ctgtatacca aacagcaatt catcaataga ttggtttttc
     4981 tgtgaaccaa tgatttgcaa atataaattt tttaagctat taaattttcg ttttgtcatt
     5041 ggtgaattag gctcgagcac aggcaactgc tctattctat gatagagttt tttggctatt
     5101 aagcattttt ctttattaaa tcctggatgg atagaaggtg gaggtggtgg taaaacataa
     5161 aagggaatat tttgactcgg tttgccatag tccaattgag ttttatacgg caggtttata
     5221 aagaaacaag aggatatcga tgaggggaca gatgtttttg taaccgcctc aattaatttt
     5281 attgctgaat gtcgacaagt attgcctgta ttgatttcat gaatttcctc attaagctcc
     5341 ttccaatggg aaccagttgc gaaaacacga gaactggttt gcgaaaaata gactgcattt
     5401 ccattttgaa caaatggttt aaaacattca aattgattgc tctctgtttg aatagattca
     5461 agataatgaa caaattcaca atactgctca taactaatat cataggcttg ataagaaata
     5521 ggcacagatc cttttcgttt tcttgtaatt ccttcatcct ctatctttgc tttgctactc
     5581 gcaaaaattg ccccacctaa agcactataa atgctaagac accttttaat acccttggtt
     5641 tgattaggca gatcaaagat tttccctact ctgcacagta gatgagatac ctggaagtca
     5701 tcatacacac ctagcataat aaatgagtgc tgattgattt tacctatcga aataaaaaag
     5761 tgatctgttc ctttattaat acagattgcc atgtgattca gtccttaaca accaataatg
     5821 ataaataata aattaacttg cacattgtaa gtgtggatgg tttttaacga taactttatg
     5881 tatattcaaa caaagctcat ggatatggtt gcccattttt gatttctcat tgcctgagcc
     5941 acctttccca ttcaaagcca caacaacagt tacattatta caaggattcc acaaatacat
     6001 caccctgtaa cctaaagtac tgccctggta agtccaaaag cgctgcctgg aatttttatc
     6061 ataataaaaa ccaactccca atccaaaacc gtagggctca tcttctgtga ctgaagggat
     6121 aggtaaccct gtttttattg aaaccactga ctccaattcg gataaagcct cgtctctaaa
     6181 aacaggttca atcagtaacc catggtatag cagctgcacc caacgaccga cgtcttcagt
     6241 atttgcgatg acagcacctg ctgcgccagc ccaggataaa tcattgctta tagtatcaat
     6301 tagctttttg ttgtcctcat cgtaaaaata accatggact cgtctatccc ggattgattt
     6361 ttcgacttct ggaccatgtg gtcctgcagg ataaaatgaa ttgtttaatc cattgtttgt
     6421 attaataatc ctttgcttta actgattttc gaaagtatct tgagtcactt tttcaataac
     6481 cattccagca agaatataat tagaattgga atactcatac ctgtttgctc tgtcggtttc
     6541 tattggcttt tctggatgag cataacctaa caactcctca tcgccccaga ttgccgcaaa
     6601 attatcctcc atcttttttg caaattcagg atcttcggaa taattgggga tgccactggt
     6661 catattcaat aattgtctta attttacatc tttccactct ttgtattgag gcagccattt
     6721 acccaaagga tcatccagtg acaatttgcc ctcagtctgc aattgaagca aaatcaatga
     6781 agtgaatgat ttggtaatgc ttccaatatc aaacaaattc aatggggtaa tttcctgaga
     6841 cagtggagga tatcccatag ttcctttgac aaaagtggca acatccaatg catcattatt
     6901 tttctcttga ggaataaaca ctgagacagc agtggcagta aatttttctt tgttatgata
     6961 aaccttgaag tacttgctca tttccttttg aattttatga tgagatacaa caggagattc
     7021 agctgcgctg atattataag ataatgaagc caggcagaag gcaagcagaa aactaactaa
     7081 gttatttatt ttcatggcga aaaatacatc aagttaagta gaaaattggc cattttaacc
     7141 tgatagactc aaatagatcc ataaaataaa aaaaacagga gatgttctgt tgaatatacg
     7201 gacacgccta ttcgcattct cttcgaccag gagcaatgaa atccaacgaa attgtagttt
     7261 tcattgccca cttgaaatat gaatacttct ccaaatacag atctttttgc attcagctta
     7321 aaatcaataa atatttagat tctaaacagc actggctctg gctcgtattt tctccaaagc
     7381 gtcttcaaga cgtttgattg atttgttttt acctaacaaa gccaatgtca tgtcaatgga
     7441 aggagatgtt ccggatccgg tcacggcgac acgcaacggt tgagcgattt tgcctaaatt
     7501 caaatcaaat tgcaggctca catcattaat gcattcttga attttatcat ttttccattc
     7561 gtcaagcgct tttaggcgct catgcaatac cattaaaggc tccagaacaa ctggccgcaa
     7621 gtgtttttta acagcatctt cattatattc aatggtatct gtataaaaat actgactaat
     7681 ttgacacatt tctgctaatg acttacatct ctctgcctga actgcgacca aatccttcaa
     7741 atcgggacct tgattcaaat caattcctgc ctgctcaaaa tgccattgca acgcattggc
     7801 aactgattct ggagagtcac ttttttgata atgctgattc aaccaataca acttatcata
     7861 attgaagctt gagacgcctc gactgacatt tttcaagtca aagctggtta tcatttcttg
     7921 tacactaaaa atttcctgat cgccatgaga ccatcctaaa cggactaagt aattcaataa
     7981 agcatgaggc aatacaccca gctccttgaa ctgcaagaca cttactgcac catgtcgctt
     8041 tgacagccgt ttgccatctt cacccaaaat catgggcaaa tgggcaaaaa caggtaccgg
     8101 cgcatccaat gccttgaata aattaatttg tctcggagta ttgttgatat gatcatcccc
     8161 gcgaataaca tgggttattt tcatatccca atcatcgata actacagcaa aattataggt
     8221 ggggtgccca tcagaacgaa ccaaaatcaa atcatccagt tcgctattat ccacatgaat
     8281 gtcaccataa acctcatcgt ggaaagacac tatacccgca tcaggatttc taaagcgaat
     8341 aacataagga actccactat caggaagagt ttggtttctg caatgcccat catagcgtgg
     8401 cttttccttg gcggctaatt gagcctctct taaagcttcc agtctctcct tactgcattg
     8461 acagcgataa gcttttcctt ctttcaaaag ttgctgcgct acttgttgat aacgagcata
     8521 tcgctcagtc tgataatagg gaccttcatc gaagttaagc cctaaccagg ccattccgtc
     8581 taaaatagcc tgtacagatt cttgagttga acgctctctg tctgtatctt ctatacgcag
     8641 tataaattgc ccattgtgat gttttgcata tagccatgaa aataatgcag tccttacccc
     8701 accaacgtgt aaaaaaccag tagggcttgg ggcaaatcgg gttctaacag tcataatatc
     8761 ttccattgta aggaataaaa taatcaagtt ataatagccg agtttaccat gaacgcaaag
     8821 attcaactgc tgaattaaga ctaaaaggag ctatgttgaa aagtattggt attaaatatc
     8881 agctaagaat cactactctt attcctgcct ttttagtcgc cttgcttttt gctttttttt
     8941 ataacggcct atttggtaaa gatctaaagc agcatatgtc cagactgggt gaagcctata
     9001 ttaggcaatt acttccagca gcccagtatg ccatgcttcg caatgattac cgtacattgc
     9061 agggtttaat caatgcctca accattaacc cggaggtaaa agctttggca ttttataatg
     9121 ctgatggtcg attaatcgct tatcgtgggg gcaagcattc tattcataaa ccattcaatc
     9181 cacctgacta tacaggtgac tacattgaaa gtaagcaaat taacccattc accattaatt
     9241 ttatagcgcc gattactatc ccaaagttca atttgtactc cagcacagaa ttcaaagagc
     9301 tttctacgcc taaaatattc caggctgacg atatattagg ctggttatcc attgacattg
     9361 atacacaatc actacttata aaacgctatc aaatgctcat cgttaccatt tttattactc
     9421 tattcggttt actgatgggt ttaacaattc attacttctt gtctaaacga atttacatgc
     9481 ctattgctcg attgcgtcga agtatgaagc aaattttaag caatgaattt gaaactgaaa
     9541 ttcgtgtttc aagtccagga gaattaggaa taattgaaaa aggttgcgct catttacaac
     9601 gacaatattt aaatacagtt cgtgatttga atcaccacat tgaaatcgcc acagcagatc
     9661 tgcaacaaag tctcgaactt ctggaagaaa aaaacatcga gctatcactg gaaaagaaaa
     9721 aaactgaaga aaaaagtcga caaaaatcag aatttattgc caacatgagt catgaaattc
     9781 gcaccccaat gaatggcgtg attggtttta caaatgtttt attggaaagc aagctggacc
     9841 ctttacaact ggactacgtt aaaacgatca aatcatcagc gcaggatttg ttaagtatca
     9901 tcaatgatat tctggatttt tctaaaattg atgcgggcaa attaaatctt gattgcatcc
     9961 cgctagacat aagaggttgc attgatgagg ttttatccct agcaagcccc aatgcacaca
    10021 aaaaaggaat cgatttaatc ccgattacag acatcaatgt gcccaaaatg gtattgggtg
    10081 accctttaag aatcaaacaa atcatcagta atctggtgac caatgcagta aaatttacag
    10141 atcatggata cgttcttata cgcactaaaa tcgagcaaga aacagataaa gattatacct
    10201 tgctatttgc cattacggat acgggaatag gtatttcacc agaggatcaa acgaaactgt
    10261 ttactgcctt caatcaggca gacaccagca tcacacgccg ttacggtggc tcaggattag
    10321 gcttggttat atgtaaaaaa ctatgtgaag aaatgcatgg acgtattagt ctaaccagtg
    10381 aaatcaacaa aggctctact tttagcgcac gcattaaagt tgaaaaactt gttgcttatg
    10441 agattgaaaa aaatcaaact catcgtttcg cccatttaaa aatcatttgc tttgatgaca
    10501 atcccctcca tttggaagca ataggtaatg gcttgggatt ttggggtata gaggctatac
    10561 gcgttgactc atttaataaa ctttcccgaa cattgacaaa acataaagat tgcaaaattg
    10621 cctttattaa cgtcaaccaa ggatgtgagc gacaagctgc tgaactcatt gccaaacacg
    10681 agcaaattcc ttttgtgtta atttccaaat ggcctattaa tgattttgcg gcgcttggtg
    10741 ctcggggctt tctttacaaa cctatcagca tccaaaaact tcaggatctg attgaatcga
    10801 tagccaatga aaaccaaact gagaaaaata cgaaccagga actggatact ttacgcgaac
    10861 agcttcgttt tcttcatcca gagatactga ttgcagaaga caaccctgtc aataaaatgc
    10921 tattaacatc cctattaaat aataatgcca atataaccac ggttgacgat ggcgaaatgg
    10981 ccgttactgc ttgtgaggat aaaaaatttg acatgatttt actggatctg catatgccaa
    11041 aacttaatgg tttggaagca gccaaaatga tacgtcaaaa atccctcatg aataaaccct
    11101 ctcctatcgt tatcattacc gccagtagca gtgatctgag ctccattgat atgaaaaaat
    11161 cgggagttga tttttgtttt caaaaaccta tagatgaaaa acaattgtta atccaaattc
    11221 ttcgcatcgt tgataaaacc aaacatgctg ccattgattg gcaattatgc gttcagaaag
    11281 tttcaggtaa tcaagcgctt gctgaagagt tcctcgccaa attcatagaa gaattatata
    11341 agaatcgtga agagtttatt gagttaatgc atcaaaaaaa cgtaaagggc ctggctgatt
    11401 tggctcataa attacatggc gcctgttgtt tttgtggtgt gcctatttta caaaagagag
    11461 tagctcaatt agagaagctt gcaagacgaa cagctaatgc tgataattta accgaagcct
    11521 ttaccgattt aatacaaagt attgatgcag tgattagtga atatgaaaat cagtattcac
    11581 aataatattt aatgaaataa ggccataaaa aactctcttg tatgaaaacc aaagtaaaaa
    11641 tctgtatcaa cctaataaaa tcattctagt tatggagaca aaatgtcagt taaaaatgca
    11701 atctacgccc agtctggtgg tgtgaccgct gtaattaatg catccgcttg tggtgtcatc
    11761 caaaccgcca aactatttcc agagcaaatt ggtaaagttt atgctgcaaa aaatggcatt
    11821 atcggtgcct taaacgaaga attaatcgat acttcattgg aaagtgaaga agacattgcc
    11881 aaattgatgc aaactccttc cggagctttt ggctcctgcc gctacaaatt aaaagacaat
    11941 ggggaagagt atcaacgctt aatcgctgtc ttcaaggccc acaacatagg ctatttcttt
    12001 tacaatggcg gcggggactc acaagatact gcccataaag tatcccagtt aggtaaaacc
    12061 atgggctatc ctattacctg cataggtatc cctaaaacag tagataacga tctgcctttt
    12121 acagacaatt gcccggggtt tggatcagtc gcgaaataca ttgccatttc aacgaaggaa
    12181 gctggttttg atgtcgcgtc catggctgct tcctccacga aagtatttat tcttgaagta
    12241 atgggaaggc atgcaggctg gatagctgcg gctagtgggc tggcagagca aaatccaggc
    12301 gaaccgccac acattattct attgcctgag gtaccttttg aaccaattcg ttttctaaaa
    12361 aaagtgaagg actgtgtgaa ttcatatggt tattgtgtga ttgttgtttc agaaggtatc
    12421 cgcaataagg aaggaaaatt tctcagtgaa gcaggattac gtgatgcatt tggacatgct
    12481 cagttaggtg gtgttgcacc agtcatcgcc caattaatta aatctgaact gggttttaaa
    12541 taccattggg cagtggcaga ttatcttcaa cgagcagcca ggcacatagc ctcgcaagtt
    12601 gatttggatc aagcttacgc tttaggtaaa gctgctgtgg aatatgccgt taaaggatat
    12661 aacgcgatta tgcctattgt cattcgtgaa caagacgaac cctatcaatg gtctataggt
    12721 caagtccctc tggttgaggt agctaaccag gaaaaggcta tgccaccaga atatatagct
    12781 gaagatggca tgggaattac ggacgcatgc aaacgttact tgtccccttt gattcaaggt
    12841 gaagcttatc caaattacaa aaatggattg ccagattatg tccgactaaa aaaccagtta
    12901 gttgctaaaa aaatcaatca agattaaggg cggcagtagg ctggacgata tttactaatc
    12961 agggtaccac cagtgcaatc ccaagtgagt attctgttag tggcatctat tgtaggaacc
    13021 attgtaatag tgccattacc tgcatttgcc gtgtaggtaa tggtaattat gccttgattg
    13081 ccaatagcta tagaggcgac actgtctgtg ggtatgggac ttgagtaact agtatctgct
    13141 tgatttaatg gcaatatatt atctatcaaa atcttctcgg caacggctaa ttttgcaaat
    13201 gaagcctttt ccaatccttc agccacccgc gctctgatgg tatagtcctg ataggctgga
    13261 aaagcgatgg caagtagaat cccaaggata gcagcaacta tcatcagctc gattaaagta
    13321 aatcctttcg ttctcatagc gccacctggc attgcatcta actgatatca cgcaaattca
    13381 atgccaactt gatgaattta aactcctctg caacctaaat gactcaatac gcctttttct
    13441 ctagatattc atgtcaggca aataactcat tagcaaagct taaacaaata aaaaaaaccg
    13501 ccattgcgag gcggttttaa taggttgatt tatcctaatt atggtctgca actggcaggc
    13561 cggtatttag gcgataagct accaccggtg cagttccagg taacatcacc gttgttggca
    13621 tccagtgtag gagtcataat aatcgtacca tcacctgctg caggggtata cgtgatggta
    13681 atcacgccgt tagcacctat tgcaatacct gtcacattag ccgtgggggc aggagactcg
    13741 tagcccgttt cagcctgagt agttggtaac gcattatttg taatagccgt ttcagatacg
    13801 gctagtttgg ctgagcttgc catctgtagc ccctcgctta cacgagctcg tatggtgtaa
    13861 tcttgatagg ctggtatagc gattgctgcc aaaataccga taatcgctac gactatcatc
    13921 aattcaatta atgtaaaacc cttttgtctc atggctatct ccatagttaa ctatccatta
    13981 gtatcatgca agcataatgc caactaccag tgtatattct aaaccaatat tattttac
//
LOCUS       DATNSN010000087        13953 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.087, whole genome shotgun sequence.
ACCESSION   DATNSN010000087 DATNSN010000000
VERSION     DATNSN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.087"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            405..1778
                     /locus_tag="VAH76_001989"
     CDS             405..1778
                     /locus_tag="VAH76_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEO1386598.1"
                     /translation="MFQSILIIALMTFSFITQAALPADPIQRCLDIRRLADITSRQKM
                     AAKEPGVLRFKFHKISASELPLDNPVGNMDEVIQALNNQIENCNNSYGEFQTVTIAGH
                     QFKRQEWCLNTNKKMLTLAKAAHGDFKKFLSSIKTEFDWYKSDGWPENHAGFKKGEFQ
                     FTAYYAPAAVEARSKRGGAFLYPLYSNPGVVSVALESKKYNLKTPLCGVDPLSKVMRG
                     FCLKNADGTYSVAPDREEIEHGALNPKYIIGYLKDPNDSAFLMLQGSGSLLLDGKLFH
                     INYDGANGRPRTMLGRIVQCAQDPTCGGNLDTMERCAKDPKCHDEAKLRCNVSKKIRQ
                     SAASEKLIRQYLNNLTSDKADNLRNRDQSYVFFAKEDGGPYGSENISLTPHVSCATDH
                     RVIPVGMNFIYHCKKATSWCVAQDAGGAIIGAHVDVYKGEGNQAGVEANQLNHTGTLF
                     VALPKRK"
     gene            2154..2444
                     /locus_tag="VAH76_001990"
     CDS             2154..2444
                     /locus_tag="VAH76_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386599.1"
                     /translation="MSIISNHDITFSSTQKDAVGKQQKNDIKNQHIADVKANKGAETS
                     EKLIVFGAKTTALAACGIALLFPLTAPMIIPIAQVSGAVALVIITRKFIKSS"
     gene            complement(2675..2821)
                     /locus_tag="VAH76_001991"
     CDS             complement(2675..2821)
                     /locus_tag="VAH76_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386600.1"
                     /translation="MAKPLNGTMKSLAKDRLDITQNQANQLISEEMTLGDLRLVPKNS
                     RRPW"
     gene            complement(2920..3075)
                     /locus_tag="VAH76_001992"
     CDS             complement(2920..3075)
                     /locus_tag="VAH76_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386601.1"
                     /translation="MYRLPIFYLSEIGLSCESIQNGMVQNEGLAHLKVLEKFGPVRKA
                     TPSVCGV"
     gene            complement(3386..4795)
                     /locus_tag="VAH76_001993"
     CDS             complement(3386..4795)
                     /locus_tag="VAH76_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2546 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386602.1"
                     /translation="MEMKMSITVKSLDFDQCISNRKYKESLQTNDGRKVWDANSLFNA
                     NKEILGKNNNGDPIHVFVGSNRQNLKADLINLNAGAATLFIPVAQELCDVMGATFHPL
                     LVPDLICENAAIGDTFHSALQVIKDLNDLNSLDSKSLAELVKSALSGQLNSLHCISDE
                     SKFLMLYSQIQYMAQQYPDEKINFEFYDDKEDILKPLYEIFSRNPDLVPANVTLNIKR
                     YLNGNLMETDFNPILGLGSQQENYQNIVKWIHKQSSSNLRSGNCCQVLEMDNEKIARY
                     CRFGKDETRLKLLDSLENLAKHQVGQKDQKMDDFIKESYEKMGGSKDMDSITLQKSLE
                     EISSAIKVTEAINKVIANYRKEAKSLFSVGMNAKADRIEKALLNVPVEDRGKIFSNDK
                     ASPELIAIRAALASHRYFGKRGNVYYKDEARTVIDENKAATTYNNLRKQFANLRTQSH
                     VDAQVELEHSPEVSRTLKL"
     gene            complement(4972..5307)
                     /locus_tag="VAH76_001994"
     CDS             complement(4972..5307)
                     /locus_tag="VAH76_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEO1386603.1"
                     /translation="MTISYEEFEKVDLRSGTVVKAEPFLRARKPAYKVWVDFGADIGV
                     LQTSAQITVHYTPEALIGRMVIGCVNLGEKNIAGFLSQFLLLGFSDEKGAICLASVEP
                     HVPNGKKMH"
     gene            complement(5385..6140)
                     /locus_tag="VAH76_001995"
     CDS             complement(5385..6140)
                     /locus_tag="VAH76_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1386604.1"
                     /translation="MLLTNRIDLRSYHTESCSHTHDFAQLVLPVSGSMELEVGHYSGV
                     VNDDIGVYIAPNEQHCFAGSQKNLFLVIDATTQNSLRDEAFESNILNLTASTKKLIQF
                     THHYLAHNERDFFTDSLINQLLFHFAASSFSPEPDPVVIRAKQWIDLYFADSVDVSRV
                     AKHCYLSISQLQRRFKQMLGCGIAEYWRMKKLLHAKKLLSQKNCSIEAIAFEVGYENL
                     PAFSRRFSKVFGESASQWRTKALTAKKMREIDK"
     gene            complement(6397..6993)
                     /locus_tag="VAH76_001996"
     CDS             complement(6397..6993)
                     /locus_tag="VAH76_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386605.1"
                     /translation="MGSTLGKWIGLIAAFLFLNNGFAHASARPIICDQEYALCTSARC
                     IPTPGSAAKAICDCVVEKGNSAGYKTCEERKPVRDRYKVTSLISTFSFEQFTTKRPMN
                     CPEGLAWSNCVDMPCTVDPQNSKRALCLCTIESAQAFFTFGGDCNTNTCATGFWSGAT
                     QENSIILRNALMQEMRSKPKELPRACPAKSSQANQGQS"
     gene            7181..7294
                     /locus_tag="VAH76_001997"
     CDS             7181..7294
                     /locus_tag="VAH76_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386606.1"
                     /translation="MDGSGYPNGLKGDEITMVARMVAIADVYEAMSTNRSY"
     gene            complement(7424..8197)
                     /locus_tag="VAH76_001998"
     CDS             complement(7424..8197)
                     /locus_tag="VAH76_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEO1386607.1"
                     /translation="MKRQNTIVRFFHFIFFIQLSYLCYAKNLITDKNLTKEFATLPKH
                     CRPNITPTLPQFIIGYGSLMQEESKKEDSSMVGENFPIYVSGFQRGWIERGTPIGFST
                     IYLGVVPKKDSIINAAYFKLNDPSQIKNYDKRENTYCRIKVPRDDIQALSSINLPEGQ
                     FWIYTTSGKQLAAPSSDYPIVQSYVDIFLSGCFSLEEKYHLKNFARDCIKTTSNWSGH
                     WVNDRIYPRTAFDNIPYVAKIDPLIAVELPQFFKLIKIE"
     gene            complement(8404..9399)
                     /locus_tag="VAH76_001999"
     CDS             complement(8404..9399)
                     /locus_tag="VAH76_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEO1386608.1"
                     /translation="MQKTARRKGIVFIIISYIILSADEPVVAKLIQLGNNFTIHGRNP
                     ISFCNLLFIGSLMGALTLFFIQYKNFRTFNFKTLTRKDWAWLFTSAFFIGFLTPSLFF
                     FGIMYANIINVILLSTLNAPITLFAAWLIFSEKPNMRLILASLITISGSFVIVLIQQW
                     TATNEKPITQITSTGPLYDFLADTPNSGEICVFLGVVSSTISTLISFHAVTTLPGGIF
                     NTFRMGLGAVFFFFIALALFGWGHFSDLLSPFLWKWMILYGPIIVGMGMFFNYLGLQH
                     IKVADDVIASSLTPLFSIVFSYLILGEAPGVAQIIGGCIIFTGIVLAMREQIRHL"
     gene            complement(9845..11512)
                     /locus_tag="VAH76_002000"
     CDS             complement(9845..11512)
                     /locus_tag="VAH76_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2552 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386609.1"
                     /translation="MKEIVSLLEEDQIDFANHKRPEVLMVLKRKFRTQKDWIITSFNQ
                     YQFTNEESQKVQQAILNGDLRITDLLNRPFSHYFWSFLTFKWGEILSGGKRLSKQEVL
                     QIASCSSNGQEFARTPTQNRNRLIKGVPLVIGKVVTSMALCMVFCLGLLSTLGAPFGL
                     SLGLAIVLSLCNGIVCLLSRGKAMLDSAGFQPQKQKTFKTLEENIKEKTILDRGQKLF
                     FGVVTLLACISAGISGYAGLVGLLTFLGAGMLISNPLVLILTIGLSVVAAVSFYSFQA
                     IGIRENYTKFKNLFIEDYQKPYGLLRCALLGMVSTVFLAVYATLTWFTTVSGVNKLFN
                     ALGATPDTLLAHFIAMICTSTTMVVNTFSQVYKVFNPKTYVDLKEKFESIYQPPEENL
                     TRAQKIQHGVINMLKLFALIGDSLAYSVAGGIRSGIHIGETLGETLGAKFALTITMAI
                     LSPVILVFVSIAFTWPTVFDRKKSTPCPSELDKPLIEKMSRSQTSDVLQNNTLLSTSE
                     KSSKPDDSNIRQAGLRFFDVSLNEERTHKPNCNPTLESGTVLTSPSP"
     gene            11690..13057
                     /locus_tag="VAH76_002001"
     CDS             11690..13057
                     /locus_tag="VAH76_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386610.1"
                     /translation="MALLSFLKPAPYLDEIQDKNVVKRQYRYWRIRTFYAMYIGYALF
                     YFTRKSFTFVMPALQSTLGMTKTELGMIASILSLSYGISKFLSGILGDKSNPRYLMAI
                     GLILTGVFNILFGLSSTWWLFAIFWGLNGWFQGWGWPGCTKLLTHWYSQSERGRWWSL
                     WNTSHNVGGALIPLIVAVCAQYFGWRSAMFVPGIICILGGVFLINRLRDTPQSLGLPA
                     IEQYHEDCSGSANHRQEKTELSVKEILWNYVLCNQYIWILSISYLFIYIVRTAINDWS
                     MLYLTEVKEYSLITAGSCVIWFEVGGFFGNLVAGWSSDKIFQGKRNPINVLFTAGVFA
                     TLLLFTLTRAYTPFLDALFLFFFGFFIFGPQMMIGMACAELAHKKAAATATGFAGFFA
                     YCLGAVLAGAPLGAIIKNYGWDNFFLTLFVCCLIPLFLMLPLWHVKVNPKYRQSEFSG
                     KSEFA"
     gene            13368..13802
                     /locus_tag="VAH76_002002"
     CDS             13368..13802
                     /locus_tag="VAH76_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEO1386611.1"
                     /translation="MNYYSILLTIKRLIIGLLPILLVACTHQQSHGPDHYVVRGKTYH
                     VMKSAKHYKAKGIASWYGSHARHQKTSTGERYNMYAMTAAHPTLPIATRVRVKNLNNG
                     RSVVVRINDRGPFLSNRLIDLSFAAAKKLGIKGIAPVEIEVV"
BASE COUNT         4371 a         2661 c         2553 g         4368 t
ORIGIN      
        1 ccaataaaaa attatgggtt tattgttatg ttgaacacta tgattaatca atcgcgaggt
       61 tcaatcaaac actgaggcta accgatgcta tacgccatat ttgacaataa cttaaaaagt
      121 cttttctgga caataaagtt tcaagcttaa gaagaagtgc ttcaatcgca gataaattaa
      181 gcgcatcaac tgatgtatgt aagcatttgg tttgaaacgc tttttaaaca gggacaattg
      241 ggattcgaca ctatttctgt ataatgataa ggtggttata gggtggctga atttatgtaa
      301 attcatctct ttgatgagtt ggccaatagc ctatataatt catgttacta attgaaatca
      361 atggacgggc aatgcctcaa ccatagctaa gctaaaggat accaatgttt caatccattc
      421 taataattgc actaatgact tttagtttca tcactcaagc agccctccct gctgatccaa
      481 tacagcgctg cctggatatt agaaggctcg cggatatcac ctcaagacag aaaatggcgg
      541 caaaagaacc cggggtgtta cgttttaagt ttcataaaat ttcagcatca gaactcccgt
      601 tagataaccc tgtgggtaat atggatgaag ttatccaggc attaaataat cagattgaaa
      661 actgcaataa tagctatggc gaattccaga cagtaacaat cgctggccat caattcaaac
      721 gccaggaatg gtgcttaaat accaacaaaa aaatgctgac tctggcaaaa gccgctcatg
      781 gtgattttaa aaaattttta tcgagcataa aaaccgagtt tgactggtat aaaagcgatg
      841 gctggccgga aaaccacgca ggatttaaaa aaggtgagtt tcaatttact gcctattacg
      901 ctccagccgc tgttgaagcc cgatctaaac gtggtggagc atttctgtat cctctctata
      961 gcaaccccgg ggttgttagc gttgcgttgg aaagtaaaaa atataatttg aaaactcctc
     1021 tttgcggagt ggatccgctt agcaaagtga tgcgtggatt ttgtctgaaa aatgccgatg
     1081 gcacctactc tgtagcacca gatcgagaag aaattgagca tggggcttta aatccaaaat
     1141 atataattgg ctatcttaaa gacccaaatg actctgcttt tttaatgcta caaggctctg
     1201 gttcattact ccttgatggc aaattgtttc acatcaatta cgacggtgcc aacggcaggc
     1261 cacgcaccat gcttggtcgc atagtccaat gcgctcaaga tccaacctgt ggaggcaatc
     1321 ttgacacaat ggaacgttgc gctaaagatc cgaaatgcca tgatgaagcc aagttacgct
     1381 gcaatgtatc taaaaaaatc aggcaaagcg ctgcatcaga aaagcttatt cggcaatatc
     1441 tgaacaatct tacctctgat aaagcagaca atttacggaa tcgagatcaa agctatgtat
     1501 tttttgccaa agaagatggt ggcccttatg gttcagaaaa tatttctcta acccctcatg
     1561 tgtcatgtgc aaccgatcac agggttattc ctgttggaat gaattttatc tatcattgca
     1621 aaaaagctac ttcatggtgt gtggctcagg atgcaggcgg cgccattata ggggcccatg
     1681 ttgacgtcta taaaggtgag ggaaatcaag caggtgtgga agcaaatcag ttaaatcata
     1741 ctggaacgtt atttgtggca ctacctaaac ggaaatagaa agaaggcaat caatacttat
     1801 ttcgagtaat tacttttatt gccatagtca tttagggtgc tgttaatcta caaccgaata
     1861 aataagaatg ctttttgatt tggattactt tgcgcaatgg gcaaggaaaa tgcgactaag
     1921 cttttttcta tgatctgaga acaaaatgat tccattgggg tactgattaa catcctccaa
     1981 gctcgccatt aaaaccacat atttttaata catattcagc atttatctta tataacttat
     2041 tgaaaatcaa aagatattac aaatagtata tatcacatat gattataatt cattgtatat
     2101 tttttaatct ttatgattaa atataattaa tcagttcgct tttggagagc atcatgtcca
     2161 ttatatctaa tcatgacatc acttttagtt ccacccaaaa ggatgcggtc ggaaaacagc
     2221 aaaaaaatga cattaaaaat caacacatcg cagatgtaaa agcgaataag ggagcagaaa
     2281 catcagaaaa attgattgtt tttggtgcca aaacaacagc tcttgcggcg tgtggtattg
     2341 ctctattatt tccactaaca gcaccaatga ttattcccat agcccaagtc agtggtgcag
     2401 tggcactagt catcattact cgcaagttta tcaaaagctc ctaacagcgc ctataggcgc
     2461 tagaatgcaa tgggaaggct tgtattactt tccattgcaa tgaatgctaa tttcaaaaat
     2521 tccatgtatt tctattggag cagctctatc aggaaattca gccattaatt ttaatgatcc
     2581 tcccataact gaactatatt tactcaaagt tgaaattaac ttatcagaac atttttccaa
     2641 acgagagatt ccatcttatt tcatatgtaa tatttcacca aggtcttctt gagttttttg
     2701 gtaccagacg taaatcaccc aaagtcattt cttcagaaat gagttgattg gcttggtttt
     2761 gagtaatatc caacctatcc tttgcgagag atttcattgt gccattaagt ggtttcgcca
     2821 tgaaacaact taatgtttta aatttgataa atgttcatca aatatcaaat ctttcgttta
     2881 aattatatcg ttataaagct gcgtttcctt gccacctgat tacactccac aaacggacgg
     2941 agttgccttt cgcacagggc caaatttctc taaaactttt aaatgagcta aaccttcgtt
     3001 ttgaaccatt ccattttgta tggactcaca acttaaacct atctcactca aataaaaaat
     3061 tggcaatcta tacatgaatc tctcagatct ctgctatcaa atattacctt attattacaa
     3121 agaacattgc ttaaaaatga cagtttaatg ggggtctgtt tacatttcgt ttcgcatagc
     3181 gaaaatgtgg ataattaggt gccattatga gcttgaatga ggcgaatagc gagccctatt
     3241 taacgaattc aagctcataa tggcgccaat tagctgcatt tgcagccgtg aaatgaaatg
     3301 taaacagacc ctaaactttt ggtagtaata aaacaagccg gaactggtga acaaatccgg
     3361 ctggaataga aatttataga accatttata attttagagt tcttgaaacc tcaggcgaat
     3421 gctccagttc aacttgggca tcaacatggc tttgtgtcct taaattcgca aattgctttc
     3481 ttaaattatt gtaagtcgtc gccgctttat tttcatcaat cacagtacgt gcttcatctt
     3541 tataatagac attaccccgc ttcccaaagt atcggtgtga agccaaagct gctcttatcg
     3601 caattaattc cggagaggct ttgtcattag agaaaatttt acctcgatct tccacaggta
     3661 cgttgagtaa tgccttttct attctgtctg ctttggcatt catgccaacg ctaaataagc
     3721 ttttggcttc tttgcgataa tttgcaatga ccttgttaat cgcttcggtt acttttattg
     3781 cactactaat ttcctcgagt gatttttgca acgttataga atccatatct tttgagccac
     3841 ccattttttc ataagactct ttgataaagt catccatttt ttgatctttt tggcccactt
     3901 gatgtttcgc aagattctca agagagtcta atagttttag ccttgtctca tcttttccaa
     3961 atcggcagta tcttgcaatc ttttcattat ccatctctaa tacttggcaa caattccctg
     4021 accttaggtt gctgcttgat tgtttatgaa tccattttac aatattctga taattctctt
     4081 gttgagatcc caagcccaaa attggattaa aatcagtctc cataagattg ccattcaaat
     4141 aacgtttaat attcaaagtc acattagcag gtactaaatc aggatttcta gagaagatct
     4201 catacagtgg ctttaatata tcttccttgt catcgtaaaa ctcaaaatta atcttctcat
     4261 caggatattg ttgagccata tactgtatct gtgaatacag cattaaaaat ttactctcat
     4321 cgcttatgca atggagtgaa tttaattggc cactgagcgc agattttacc agttctgcta
     4381 gagacttact gtctaatgaa ttcaagtcat tcaagtcttt aatcacttgc agagcgctgt
     4441 gaaaagtatc tccaattgcg gcattctcac agattaaatc aggaacaagt aatggatgaa
     4501 aagttgcccc catgacatcg cacaactctt gagctacggg tataaataaa gtagctgctc
     4561 ctgcatttaa attgattaaa tccgctttga gattttgcct gttagaacca acaaaaacat
     4621 ggatgggatc gccattatta tttttaccaa gaatttcctt attggcgtta aataaactat
     4681 tggcatccca aactttcctc ccatcatttg tttgtaatga ctctttatat ttcctgtttg
     4741 agatacattg atcaaaatcc aaggatttaa cggttatgct cattttcatc tccaagataa
     4801 caataattat ttcgatatgt ttaattattt tgcatgttgt gctgttattt tgaaaacgaa
     4861 ttatatttat aactgatatc agtaatttta atatcaataa atttaatcaa tagggctaaa
     4921 aaaacaaatc gttagttcag atttgtgatg tatcaatatt tcagatagga atcagtgcat
     4981 ttttttacca ttaggaacat ggggctcaac ggaagccaga catatagcgc ccttctcatc
     5041 ggaaaaacca agcaatagaa attgagagag aaacccggca atatttttct cccctaaatt
     5101 cacgcatcca atgaccatcc gccctatcaa tgcttccggc gtataatgca ctgtaatttg
     5161 cgcagaggtt tggagaacgc caatatcggc accaaaatcc acccaaactt tataagcagg
     5221 tttcctggca cgcaggaatg gttctgcttt cacaacggta ccggaacgca aatcgacttt
     5281 ttcaaattct tcataagata tcgtcatgtt gcacctcaat gttttatata ggctgtatcg
     5341 ttaccaccaa atgaattcgt agtctatcaa tttaaaacca gagcttattt atctatttca
     5401 cgcattttct ttgctgttaa cgctttggtt ctccattgag atgcagattc accgaatact
     5461 ttactaaaac ggcgactgaa tgcgggcaag ttttcataac ctacttcaaa tgcgattgct
     5521 tcaatggaac aatttttctg agataataat tttttagcat ggagtaattt tttcatccgc
     5581 caatattcgg caataccaca gcctaacatc tgcttaaaac ggcgctgcaa ttgactaatg
     5641 ctcaaataac aatgtttcgc taccctgctc acatccacag aatctgcaaa ataaagatca
     5701 atccattgtt ttgctctgat aacaaccgga tccggttcgg gtgaaaaaga acttgccgca
     5761 aagtgaaaca ataattgatt gattaaagag tctgtaaaaa aatccctttc attatgtgct
     5821 aaataatggt gggtaaattg gatgagcttt ttagtactgg cggttaaatt gagaatatta
     5881 gattcaaaag cctcgtcacg aagagagttt tgagtagttg catcaatcac taaaaacaga
     5941 tttttttggc ttccggcaaa acaatgttgt tcattaggcg caatgtatac tccgatatca
     6001 tcattaacga caccggagta gtgacctacc tccagctcca tagaacccga gacgggcagt
     6061 accaattgcg caaaatcgtg agtgtgactg caactttcag tatggtaaga acgaagatca
     6121 atacgattag tcagcaacat attgctcccg agcgaacatt tacctttaaa agaaacagat
     6181 tatatcatac ttgatttgaa acccattgag agaggtagcc aaaactttgg attagcttga
     6241 gcttttattt cataaaaccc aaagggatag gtcggaactg acagaggctt aaaatgcctg
     6301 accaaagctt attgaaaata gacctcgtga ttgctcagga tttgctcttg ttatattttg
     6361 gacataacag attctttgga tcattataaa ttttaatcaa ctctgcccct ggtttgcttg
     6421 tgatgattta gcagggcaag ccctgggtag ttccttaggt ttcgaacgca tttcttgcat
     6481 taatgcatta cgaagaatga tactattctc ttgggttgcg ccagaccaaa agccggtagc
     6541 acaagtattc gtattacaat caccgccaaa agtaaaaaaa gcttgagcgc tctctatcgt
     6601 acaaagacac aaagcacgtt ttgaattttg tggatcaacc gtgcacggca tatcaacaca
     6661 attcgaccaa gctaatcctt ctggacaatt cataggtctt ttagtagtaa attgctcaaa
     6721 tgaaaaagtt gaaataagtg atgtcacttt gtatctgtct cgaacaggct ttctctcttc
     6781 acaagtttta taaccagcac tgttaccttt ttctactacg caatcacaaa ttgctttggc
     6841 cgcacttcca ggtgtaggaa tacagcgagc agaagtacaa agcgcatact cctgatcaca
     6901 tataattggc cttgcagagg cgtgtgcaaa accattatta agaaataaaa aagcagctat
     6961 taatccaatc cattttccaa gtgttgagcc cattgttgag gtccctctgt tcttttgttt
     7021 atcacagtat ctttatcata ttatcgaatt ttcataattc aaaaaaagca ttggagttta
     7081 ctatactgaa tatattatta agggaccaaa tgagtaagat aattgaacat atgaattttc
     7141 cttgggacat taaatctgta gttaccaaca tcatgaacgc atggatggtt ctggttaccc
     7201 caacggactc aaaggggatg aaatcaccat ggtagcacgc atggtggcta ttgcggatgt
     7261 gtatgaagcc atgtcaacga atagatctta ttgacatttc atgttgcaaa taactatctt
     7321 aaagcatctg gaggaaggta aagaggtttt gtctgatacc agttatgttg agtgaatacg
     7381 tgaaaggggg cagatacaac ataaaaagct atcattgctt acttcactct attttaatta
     7441 atttgaagaa ttgtggaagt tctactgcaa taagaggatc aatttttgcg acatagggga
     7501 tattatcaaa tgcggttctt ggataaattc tgtcgttgac ccaatgacct gaccaattgg
     7561 acgtggtttt aatgcaatcc ctcgcaaaat tctttaaatg atatttttct tctaaactaa
     7621 agcaacctga taaaaaaata tcaacataag attgcacaat aggataatca ctggaaggag
     7681 cagcaagttg tttcccactc gttgtataaa tccaaaactg cccttcaggt agattaatgg
     7741 agctcagggc ttgaatgtca tctcgtggaa ctttaattcg gcaataagta ttttcacgtt
     7801 tgtcgtaatt cttaatttga gatggatcat ttaacttaaa ataagccgca ttaatgatac
     7861 tgtctttttt gggtactaca ccaagataaa tggtactaaa tccaatcggt gtgcctcgtt
     7921 caatccaccc cctttgaaaa cctgatacat aaattggaaa gttttctcct accattgagc
     7981 tgtcttcttt tttggattct tcttgcatta acgatccata gccaatgata aattgtggca
     8041 aagttggagt aatattaggc cggcaatgct taggtagtgt agcaaactcc ttagttaaat
     8101 ttttatccgt tattaaattt ttggcataac ataaataact cagttgaata aaaaaaataa
     8161 aatgaaaaaa tcttactata gtattttgac gtttcatttt tatgatccta ttaaacaatg
     8221 cgcccgatgc ccgaagagct tataaccttg catccatcac gtttctatca taaataatac
     8281 tctcgttacc tatttataga gttaggatag atgccatata gaaaagaaaa gcttcctagg
     8341 ttactctttg tcaatttttc atgcatccct tttggggaaa aggtaaaaat ataaacagct
     8401 atctcaaaga tgccgtattt gttctctcat ggctaatact attcccgtaa aaataataca
     8461 acctccgata atttgagcaa ccccgggagc ttctccaaga attaaataag agaaaacaat
     8521 ggagaaaagg ggtgtcaaag aggatgctat cacgtcatct gcgactttaa tatgttgcag
     8581 ccccagatag ttaaaaaaca tccccatacc tacaatgata ggcccatata atatcatcca
     8641 cttccaaaga aatggtgata aaagatccga aaaatgcccc caaccaaaca gtgctaaagc
     8701 aataaaaaag aaaaaaacag cccctaatcc catacgaaaa gtattaaaaa taccaccagg
     8761 taatgttgta acagcatgaa aacttatcaa ggtggaaatt gtacttgaga caacacctaa
     8821 aaatacgcaa atttccccag agtttggtgt gtctgcaaga aaatcataaa gaggtccagt
     8881 cgatgtgatt tgggtaatag gtttctcgtt tgtagctgtc cattgctgaa taagaacaat
     8941 cacaaaacta cctgagatag tgattaggga agctaaaatt agccgcatat taggtttttc
     9001 agaaaaaata agccatgcag caaataaagt tattggagca ttaagagttg agagcagaat
     9061 tacattaata atatttgcgt acataatacc gaaaaaaaat agcgagggtg ttaaaaaacc
     9121 tataaaaaaa gcgctggtaa atagccatgc ccaatctttt ctggttaatg tcttgaagtt
     9181 gaatgttcta aaattcttat actggataaa aaatagcgtt agagctccca ttagactacc
     9241 tataaagagg agattacaaa atgagatggg attacgtcca tggatagtga agttattacc
     9301 aagttgaatc agtttggcaa cgacaggctc atccgcactt aatataatat aactgattat
     9361 tataaaaact attcccttac gtctggccgt tttctgcatt attccctgta cttaaaactt
     9421 tccttaacgt cattgtaaaa tatttacagt tcatggtcac gaacctcagg aaaggggctt
     9481 tccttttgct tttcagaaaa agggaaaggc ctgttcctaa gtttataagt tataataaga
     9541 ctggttccaa aggcaacgac gccagatctt gtcttttgcc tatcaaagta gttctgggtc
     9601 attttagaga aagcaaaaaa caagattttc tccccctgct ccgttaaatt ccataatttt
     9661 agagagctta gacttacaaa ataattgata ttttcagcaa atcaaaaacc atcgttgaat
     9721 gactctggag caattacaag aaaagaacct gcaaattagt tgtttaacaa gtatcgatta
     9781 aatttactct tttccagatt caaatcaatc gatacttcac tagttgattc aaaaacctca
     9841 tcacttatgg cgatggagat gtcaaaacgg ttccgctctc taaagtaggg ttacaattag
     9901 gcttatgagt acgctcttcg ttcaagctca catcaaaaaa tcttagccct gcttgcctga
     9961 tatttgaatc atcaggtttg gatgattttt ctgatgttga taatagagta ttattttgca
    10021 gcacatctga agtttggctg cgactcattt tctctatcaa tggcttgtcc agctctgaag
    10081 ggcatggagt cgattttttt ctatcaaaaa cagtaggcca ggtaaaagct atcgatacaa
    10141 atactaaaat gaccggagac aatattgcca tggtaatggt taatgcaaat tttgccccta
    10201 gtgtttctcc cagcgtttca ccaatatgaa tcccgcttcg gattccaccg gcaacagaat
    10261 aagcgagaga atcacctatt aaagcaaata atttgagcat gttaataact ccatgctgta
    10321 tcttttgtgc tcttgttagg ttttcttcag gtggttgata tattgattca aatttttctt
    10381 ttagatcgac atacgttttc gggttaaaga ctttatatac ttgtgaaaaa gtattgacaa
    10441 ccatcgttgt tgaagtacat atcatggcaa taaaatgtgc taaaagagta tcaggagtcg
    10501 cacctaaagc attgaaaagt ttattaaccc ctgagaccgt tgtaaaccaa gttaacgtcg
    10561 cataaacagc caaaaaaacc gtacttacca ttcctaataa agcacatcgc aaaagaccat
    10621 agggtttttg gtaatcttca ataaaaagat ttttaaattt tgtataattt tcacgtatgc
    10681 cgatagcttg aaacgaataa aaagagaccg ctgcaaccac agacagccca atggtaagta
    10741 tcaaaaccag agggttagag attagcatgc ctgcccccaa aaacgtgaga agtcctacaa
    10801 gaccagcata tccagaaatg cccgcagaaa tgcacgccaa caaggtcact acaccaaaaa
    10861 ataatttctg gcctctatcg agtatcgttt tttcttttat attttcttca agggtcttaa
    10921 acgttttttg tttttgaggc tgaaagccgg cactatcaag catggctttc ccgcggctta
    10981 ataagcaaac tattccatta cataaggaaa gtactatcgc caagccaagg ctaaggccaa
    11041 aaggcgctcc taaagtactt aataatccca gacaaaatac catacacagc gccatggagg
    11101 taacgacttt gccaatgact aatggcactc cttttatcag cctgtttcta ttttgagttg
    11161 gtgttctcgc aaattcctgg ccattgctag agcagctagc aatctgtaag acttcctgct
    11221 tagataatct ttttccaccc gaaagtatct ctccccactt aaaggtaaga aaactccaaa
    11281 aatagtgaga aaaaggacga ttaagaagat ctgttatgcg aagatctccg tttagaatgg
    11341 cttgttgaac tttttgcgat tcttcgttag taaattgata ttggttgaaa gaggtaataa
    11401 tccaatcttt ttgagtacga aattttcttt ttaaaaccat taacacttcg ggacgtttat
    11461 gattagcaaa gtcgatttga tcttcttcta ataaactaac tatttctttc attacaataa
    11521 atcctttttt aatacaaact gcgtagctta tttataaatt cacaagaatt ccattaaatt
    11581 tttatttcaa tcatcaatag gtatgatcct gttaaatttt cactagaaag atttgacata
    11641 ctttgcgagt tacattctaa tcatgttttt ttcatgatca tggacaatca tggcattatt
    11701 gagttttcta aaacctgctc catatcttga tgaaattcag gataagaacg ttgttaaacg
    11761 gcaatatcgt tattggcgta ttcgcacctt ttatgcaatg tatatagggt atgcgttgtt
    11821 ttattttacg cgaaagagct ttaccttcgt gatgcctgcg ttacaaagca ccttggggat
    11881 gactaaaact gaactgggaa tgatcgccag cattttgagt ttaagttatg gtatcagcaa
    11941 atttttgagt ggaattcttg gtgataaatc taatcctcgt tatttaatgg cgatcggact
    12001 gattttgaca ggtgttttta atattttgtt tggattgtct tctacctggt ggttattcgc
    12061 tattttttgg ggattgaatg gttggtttca agggtggggc tggccaggat gtactaaatt
    12121 attaacacat tggtattctc aatcggagcg tggccgttgg tggagtcttt ggaatacatc
    12181 acacaacgtt ggtggtgcat taattccttt gattgttgct gtgtgcgcgc aatacttcgg
    12241 ctggcgttca gccatgtttg tgcctgggat catttgcatt ttaggaggtg tttttttaat
    12301 caatcgttta agagataccc cacaatcact tggtttaccc gcgatagagc aatatcatga
    12361 agactgttct ggttccgcaa atcaccgcca ggagaaaaca gaactctcag tcaaagaaat
    12421 tctttggaat tatgtgttgt gcaaccaata tatttggata ttgtccattt cttatttgtt
    12481 tatttacatt gtccgaacgg ccattaatga ttggagcatg ttgtatttaa cggaagtgaa
    12541 agagtattcc ttaatcactg caggcagttg tgtcatatgg tttgaagttg gtggcttttt
    12601 tggaaatctg gttgccggtt ggtcttctga caaaatattt caaggtaaac gcaatccaat
    12661 caacgttctt tttactgcag gcgtatttgc cactttattg ctgtttacgt taaccagggc
    12721 atatacaccg ttcctcgatg ctttgtttct ttttttcttt ggttttttta tctttgggcc
    12781 ccaaatgatg attggtatgg cctgtgcgga attagcacat aaaaaagcag cggcgactgc
    12841 aactggcttt gccggatttt ttgcttattg tttaggtgct gttctggccg gtgctccttt
    12901 gggtgcaatt atcaaaaatt acggctggga taatttcttc ctcacattgt ttgtttgctg
    12961 cctgattcca ttatttttga tgctgccgct ctggcatgtg aaagttaatc caaagtatcg
    13021 tcaatcagaa ttttcaggca aatccgaatt tgcttagtga ttggcttccc tgggtaatcc
    13081 agaaaaagta ggtcgggcct gccttggccc gatctgctct tgctacctcc acatgaaagc
    13141 ccctggggta tagagatatc cttgttgtga taggacaaga ggttatacag aaaaatttgt
    13201 tggtacatat tcatccaaaa aatgtataat caatcaaatc aatgatgaaa gcaaatgata
    13261 aaaatgtgtt acctattgac gattttcttt gcaagaaata tctttgattc tcgtgatcta
    13321 tgaattacgt atatctaaaa tctgtagcgg aagtagaaga taactttatg aattattatt
    13381 cgattttgtt gaccattaaa cgactcatta taggtttact ccccatacta ctggttgctt
    13441 gtacgcatca gcaatctcat ggtccggatc actatgtggt cagagggaaa acatatcatg
    13501 tgatgaagtc tgccaaacac tacaaagcaa aaggaattgc ttcctggtat gggtcacacg
    13561 ctcggcacca aaaaacatcg accggagagc gctacaacat gtatgcgatg acagccgcgc
    13621 atccaacact cccaattgct acacgagtac gggtcaaaaa cctgaataat ggccgatcgg
    13681 ttgtggttcg aattaacgac agaggtcctt ttttatcaaa caggctaatt gatttatcct
    13741 ttgctgccgc taaaaaactc ggtataaaag gcattgctcc tgttgaaatt gaagtcgttt
    13801 gaaaatgcta agaagtaaag cttactcaag ctaataacct gatttacctg gttaacttat
    13861 taaataatct aaaattaaga aatcgtccta ttcatcaatg aatttcgtac tcatttagac
    13921 tttaaaataa ctcatagtaa attaagtact tgc
//
LOCUS       DATNSN010000088        13824 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.088, whole genome shotgun sequence.
ACCESSION   DATNSN010000088 DATNSN010000000
VERSION     DATNSN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.088"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(88..2022)
                     /gene="dnaK"
                     /locus_tag="VAH76_002003"
     CDS             complement(88..2022)
                     /gene="dnaK"
                     /locus_tag="VAH76_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO1386612.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGDKPKVIENSEGHRTTPSIVAFTD
                     DNEILVGQSAKRQSVTNPEKTLFAIKRLIGRRFDDPIVQKDIKMVPYKIMKADNGDAW
                     VRVKDQDKAPPQISAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGMDKKRGDSVIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLALIEYLASEFKKDTGIDLHNDPLALQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEKLVERTIEPCKTALKDAGLTVSQI
                     NEVILVGGQTRMPLVQKTVEEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGEVKDILLL
                     DVTPLSLGIETMGGVMTKLIEKNTTIPTKATQVFSTADDNQTAVTVHVLQGEREQASA
                     NKSLGRFDLRDIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKAQSIVIKASSGLS
                     EEEVAAMVKDAQSHAEEDKKFKEMAELRNQADSLIHSCEKSMKDLADELSEDEKKGIE
                     TAISELKEAVQGTDKARIEDKLKVLTDASAKMAERIYAKKSSEGQAAQGQTQSQESTK
                     PAEEGVVDAEFEEVKEEDKK"
     gene            complement(2167..2766)
                     /gene="grpE"
                     /locus_tag="VAH76_002004"
     CDS             complement(2167..2766)
                     /gene="grpE"
                     /locus_tag="VAH76_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO1386613.1"
                     /translation="MSKQNKKDWKKFKDEHKEEYKVENEILEEETDEESQHQEPALGH
                     PSYTALEEQLTLAEQKAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALL
                     PVMDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQAFDPQQHEA
                     MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK"
     gene            3045..4382
                     /locus_tag="VAH76_002005"
     CDS             3045..4382
                     /locus_tag="VAH76_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO1386614.1"
                     /translation="MQEWSPTTWQKYSYLQAASYADEEQLNKVVEQLSLLPPLVTSGE
                     IKHLKNEIAQAGRGNAFILQGGDCAESFNDCRSEVISNKLKIILQMSLILLYGLRKPI
                     IRIGRIAGQYAKPRSSDYETINGVTLPSYRGDIVNSPEFTATAREPNPKLLLQAYSCS
                     AMTLNFIRALLDGGFADLHHPQRWDLGFVEHSPQKNEYQHIVDSIEDALDFLNAIDGI
                     HSSSISKVDFYTSHEALHLHYEQALTRQLKDGKWYNLSTHLPWIGMRTAQTDSAHLEF
                     LRGVQNPIGIKIGPAATPEWLSEVLSVANPQKEEGRVLLYTRLGAKLIDRLLPPLIDT
                     VRKSKIPVTWSCDPMHGNTETTEDGTKTRHFDNILSELKQALEIHRSMGSYLGGVHFE
                     LTGDNVTECIGGARGLAPHDLKTAYHSLVDPRLNYEQSLEMAIQLSHQFRSEP"
     gene            4652..5791
                     /locus_tag="VAH76_002006"
     CDS             4652..5791
                     /locus_tag="VAH76_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEO1386615.1"
                     /translation="MKDTVFQEIIKPITTDLLKECVTIFKSDYDYEKFKTYEHLQSML
                     YVHLNQISSLRTLETAINSQDLGLSAKICRSTLSDANRRRKADCFLWILEQLLEMLPK
                     KQKKEFSKIVRVLDSSPIQLKGYGYEWAKHNATRRCEGLKLHVEYDLGLESPTRVALS
                     FPNFNDSSMGKQWPIETDIIYVFDKGYCDYDWWWSIHQKKAFFVSRLKVNAAISIEQK
                     FETNENSPILEDGLFRFSNPKPRGGKKNLYTSLARRISVQREDKDPLILVTNLLDEPA
                     EMIAQLYKSRWEIELFFKWIKQRLKIKKFLGKSENAVKIQLITAIIAYLLVFLFKNSH
                     NQTTPLYLMLVWIKCNLEKPVLFNAFYLDKKPMSPEVKYLLETKT"
     gene            complement(5938..6996)
                     /locus_tag="VAH76_002007"
     CDS             complement(5938..6996)
                     /locus_tag="VAH76_002007"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEO1386616.1"
                     /translation="MFDLNQSLFLRALRRQPVERTPIWIMRQAGRYLPEYRKVREHAG
                     DFLNLCKNPELACEVTLQPLRRYALDAAILFSDILTIPDAMGLGLYFAEGEGPCFTNP
                     LQDTKAIHTLKIPSIPESLSYVFDAARLIRQEMPKELPLIGFSGSPWTLACYMVEGGS
                     SRDFKRILNLIYTEKEAAHLLLNKLAVSVTAYLIEQIKAGVNAVMIFDTWGGVLTPQN
                     YKDFSLAYMHQIVQQLKKEYPDIPVILFTKNGGQWLEWMAETGCDALGVDWTCDLASA
                     RKRVGGKVALQGNLDPAVLLTTKNCIRREVGSVLASYGYGTGHIFNLGHGITPDVPPE
                     NVAIMIEAVHEISPQYHL"
     gene            7236..7574
                     /gene="folB"
                     /locus_tag="VAH76_002008"
     CDS             7236..7574
                     /gene="folB"
                     /locus_tag="VAH76_002008"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO1386617.1"
                     /translation="MDTLNITALSVETKIGVYGWEQRINQRLLIDISIPFDFKDCKDN
                     LSNTLDYADLCQTVTQFVESTSFQLIETVAEQIAQLIKQKYPVSKVSVAVTKPHAVKN
                     AAGIQVVVNR"
     gene            complement(7614..8309)
                     /locus_tag="VAH76_002009"
     CDS             complement(7614..8309)
                     /locus_tag="VAH76_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEO1386618.1"
                     /translation="MAKDYGNRRAARRNRGPHQFLVIIVTFLFGYITASFLDVQTISQ
                     WVNTQVLAQHDDEKAPTKVAQEHKQVPPKPKFEFYTLLANERGPASQASSQQNTDNHA
                     ADKTSTPIQKTANTVTAAVNNSVKSTIANPTPQTPVKVAEAKPTTMQQPNKGAYLVQV
                     ASFKARQDAEQMKGMLILKGFDVSVVPVTQAQGNWFRVVVGPYPNKALAQKAQMNLAK
                     TEHLNGMVRSVGG"
     gene            complement(8309..10078)
                     /locus_tag="VAH76_002010"
     CDS             complement(8309..10078)
                     /locus_tag="VAH76_002010"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO1386619.1"
                     /translation="MRSMKAIIEYLLKQALINLQQSGEMPIDLEIEIKVENAKDPAHG
                     DYATNLALVLAKPCRQAPKVLAERLVAVIPSDPSVEKIEIAGAGFINFFMRNTARSLI
                     ISEILNKGKEFGRGNLGQSQKVLIEFVSANPTGPLHVGHGRGAAFGATLGNVLKAAGY
                     DVTLEYYVNDAGRQMNILAVSVWLRYLELAGEPIVFPANGYKGQYVYEIAQEMWSEQG
                     NQFVHPWISVVENLPADEPEGGDKETYIDAIIARAQSLLGKDGFANFHQHALKTVLDD
                     IKDDLQAFGVRFDSWFSEQSLFEDGSIEKGIQALKDRGHTYEREGALWFRATDFGDEK
                     DRVLVRANGQTTYFASDVAYHWNKYDRGFDRVIDIFGADHHGYVTRIKTAVKALGHDE
                     SALDVILVQFAILYRGGDRVQMSTRSGSFVTLRELREEVGNDAARYFYVARKPEQHMD
                     FDLDLAKSESSDNPVYYIQYAHARICSVLRQLKERGLKWDKDMGLKNLDLLEQQHETT
                     LISLIARYPEVIQSAAASCEPHQLAYYLRELANGLHSYYNAIQLLCEQEQLRCARLCL
                     LESVRQVLNNGLAILGVSAPESM"
     gene            complement(10182..11240)
                     /locus_tag="VAH76_002011"
     CDS             complement(10182..11240)
                     /locus_tag="VAH76_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO1386620.1"
                     /translation="MNRIITFTAGLILVWITGYLLIAGRGLLIPIVISIFVWHLLNTI
                     NSGVQKIPFIGTHLPSWIRMILSLFVVVCLVMVLINIITNNVSDVIAASPRYQENLLH
                     IFNSLDQRFHVKGIFDFDSLIQGLSIQSMLVNIYGVFTTITSSAVLISLYVVFLFVEQ
                     HYFTQKMDAFFPQAEHRKLVKNIISHITKDTQTYLGIKTLLSLLTATLSWFIMKWVGL
                     DFAEFWALLIFFLNYIPNIGAIVATAFPAALALIQFESWWPFLEVTSGIVAIQFFVGN
                     FLEPRLLGKSLNLSPLVILFALALWGAIWGVLGMFLSVPITVMMMIIFAHFDVTRPIA
                     ILLSQDGYIHKSYESLEN"
     gene            11387..13459
                     /gene="recG"
                     /locus_tag="VAH76_002012"
     CDS             11387..13459
                     /gene="recG"
                     /locus_tag="VAH76_002012"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO1386621.1"
                     /translation="MLSNSCEYLTGVGPTIAAKLAKCGIFTIQDLLFHLPYKYQDRTR
                     ITPIQDLRSNEWCVIAGHVCKTEIKYGKRMMLNCYVEDKTGIVKLRFFHFNKQQIQAL
                     NNSAMIRAFGEVRGFNNQLEMIHPEYQLIDQESDFHVEETLTPIYPSTQGLTQTRLRQ
                     LVKIALEQSEHELHQLEWMSEKQLQENNFYDLGEAIKLLHNPPPDISLSNLEAGEHPA
                     LKRLIFDELLAQQLSMQFARQSRSKLQAPAILFDNAIHKRFIESLPFSLTNAQQRVFK
                     EISVDLTQSKPMLRLLQGDVGAGKTIIAALAALQAISQGFQVAFMAPTDLLSEQHTNS
                     LSKWLEPIGINVLRLSGKMKTTERKNALAALQDNSCQLIVGTHALFQEQVEFARLGLV
                     IIDEQHRFGVEQRLLLQQKGQLNQLIPHQLLMTATPIPRTLSMSHFAHLDISVIDELP
                     PGRMPITTAVLNQDKRELIIERLQAAIANGKQAYWVCTLIEESEKLQCIAATDTSKKL
                     QEQLSCARVGLVHGRMKALEKEATMAAFKQGEIDLLVATTVIEVGVDVPNASLMIIEN
                     AERLGLSQLHQLRGRVGRGNNQSHCLLLYQSPLSQQGAERLKIMRSTTDGFLISEKDL
                     ELRGSGEILGTRQTGFRQFKIANLQRDKTLFAILRPIAKQLMLEKTAIARQITQRWLG
                     NFEQFLQS"
BASE COUNT         4298 a         2961 c         2473 g         4092 t
ORIGIN      
        1 atgtcgcccc gacccaaaat ttatttatta taacccgata aatccttctt ccgccctctt
       61 aaatcagagg gcgagagtaa tattaaatta ctttttatcc tcttctttaa cttcttcaaa
      121 ctcagcatca acaactccct cttccgcagg ttttgtagat tcctgagatt gtgtttgccc
      181 ctgagctgcc tgaccttcag acgatttttt agcataaata cgttcagcca tttttgcgga
      241 agcgtctgtc aatactttca gcttgtcttc aatacgagct ttgtcagttc cttgcaccgc
      301 ctctttcagc tcagaaattg ctgtttcgat gccttttttc tcatcctcag acaactcatc
      361 agccaaatct ttcatggatt tttcacagct atgaatcaaa ctatctgcct gattgcgcag
      421 ttctgccatt tccttgaatt tcttatcttc ttcagcatgc gattgggcat ctttcaccat
      481 agcagcaacc tcttcttcac ttaaaccact ggaagctttg atgacaatgg attgtgcctt
      541 gccagttgcc ttgtctttgg cagaaacatt caagatacca ttggcatcaa tatcaaaagt
      601 tacttcaatt tgaggaacac cacggggagc aggagggatg tcacgcaaat caaaccgtcc
      661 cagtgacttg tttgctgaag cctgttctct ttcaccctgt agtacatgca cagtcactgc
      721 tgtctgatta tcatcggctg ttgaaaacac ttgtgtagcc tttgtaggaa tagttgtgtt
      781 tttttcaatg agtttggtca ttacaccacc catggtttct ataccgagag acaaaggagt
      841 gacatcgagt aaaagaatgt ctttcacttc accggacaat actgctgcct gaattgcagc
      901 acctactgcc acagcctcat cagggtttac atccttacga ggttctttgc caaagaattc
      961 ttccacagtt ttttgtacca gaggcatacg agtttgccca ccgaccagaa taacttcgtt
     1021 gatttgtgag acagtaagcc ctgcatcttt taaagctgtt ttacatggct caatagttct
     1081 ctcaaccagt ttctccacta aagattccag ttttgcgcga gtcaatttga tatttaaatg
     1141 ttttggtcca gaagcatctg ctgtaatata aggcaaattg acatcggttt gttgtgcaga
     1201 tgataattca atttttgctt tctcggcagc ctctttcaag cgttgcagag ccagaggatc
     1261 attatgcaaa tcaataccag tgtctttctt gaactcagaa gctaaatact caattaaagc
     1321 caaatcaaaa tcttctccac cgaggaaagt atcaccattg gttgcaagta cttcaaattg
     1381 atgttcgcca tccacttcgg caatttcaat aattgaaata tcaaaagttc ctccccctaa
     1441 atcatatacc gcaataacag agtcaccgcg ttttttatcc ataccataag ccaatgcagc
     1501 agcggtaggc tcattaatga ttcgttttac ctccaggcca gcaatacgac cagcatcttt
     1561 ggttgcctgg cgttgcgaat cgttgaaata ggcaggtaca gtaatcactg cttcttttac
     1621 ttcttcaccc aaataatcct ctgctgtttt tttcattttt cttagcactt cagcagaaat
     1681 ttgtggtggt gccttatctt gatccttgac tctaacccaa gcatccccat tgtccgcttt
     1741 cataattttg tagggaacca tcttaatatc tttttgcaca ataggatcat caaatcgacg
     1801 cccaattaat cgtttaatcg cgaacagtgt tttctctggg ttagtcacgg attgacgctt
     1861 ggctgattgg cctaccaata tttcattatc atcagtaaat gctacgatag aaggagttgt
     1921 tctgtgacct tcactatttt caattacctt cggtttgtca ccttccatga cagccacgca
     1981 agagtttgta gtacccaggt ctatacctat aattttagcc attctatttt gctccaaatt
     2041 aatattttct catgcaatat tccacatatg gggtgtatca tgatgatttc aagccaccac
     2101 cctaaaaaaa gctgaataca ctgcaactaa aaatttgttt aaaaacacct caactgaaac
     2161 accagattat ttggtggaaa ctatcacccg agctggtcga attactcgat cgctcaattt
     2221 gtaacctttt tggaatacag taattacaga attcggtggt gctcctgggg caggttgcat
     2281 tgacattgcc tcatgttgtt gagggtcaaa tgcctgtccc agaggatcaa tttgttccac
     2341 atcaaacttc tgtaatgcgt caagaaagag tttcatggtt aactcaagcc cttcatgcat
     2401 agatggatca ctgttcttat ccgctaattg caatgcttgc tcaagactgt ccattactgg
     2461 caataaagct gaaatcagtt tttctacgcc atatttatgg gcattggcaa cttctcgttc
     2521 catacgacga cgaacgttct ccaattcagc aagggcgcgt acagattttt cccaattctc
     2581 atgcgctttt tgttccgcca atgtcagttg ctcttccaag gcagtatagc tgggatgtcc
     2641 taaagcaggt tcttgatgtt gtgattcttc atcagtttct tcttccagaa tctcattctc
     2701 taccttgtac tcttctttgt gttcgtcttt aaattttttc caatcttttt tattttgttt
     2761 actcatcaaa atcgctcctg actaactcga ttcctacaaa tggggattgt atttgcaatt
     2821 ttcaagcccc tgttaggatt ttaaatcttg gtttaaaatt caggtcatta actgattgaa
     2881 acttttcaaa tcatggcttg cagtaattgt ccatttattc catacttagg tagctaaaaa
     2941 atctgtctta attctcattt aggtaaaaaa acaaagtatt taataatcct ttgagttaaa
     3001 ttaagtctaa acgacaacta aagcaaaaaa aacttggata aattatgcaa gaatggtcgc
     3061 caactacatg gcagaaatat tcgtatttgc aggcagcttc gtacgctgat gaagagcaat
     3121 taaataaagt ggtagaacaa ttaagtttgc tacccccatt ggttaccagc ggtgaaatca
     3181 aacatttaaa aaatgaaatt gcccaggctg gccgaggtaa tgcttttatt ctgcagggtg
     3241 gtgattgtgc tgaatctttt aatgactgtc gttcagaagt cataagcaac aaattaaaaa
     3301 tcattcttca aatgagccta atcttattat atggcttgcg taaaccaatt attcgtatcg
     3361 gaagaatagc tggacaatat gcaaaaccac gctcctcaga ttacgaaacc ataaatggag
     3421 ttactttacc cagttatcgt ggagatatcg ttaattcacc tgaatttact gccactgcca
     3481 gggagcctaa tcctaaatta ttacttcagg catacagttg ttctgccatg actttaaatt
     3541 ttatccgagc gctgctggat ggcggttttg ctgacctgca tcacccccaa cgctgggatt
     3601 taggttttgt tgaacactcc cctcagaaga acgaatacca acatattgta gactctattg
     3661 aagacgcctt ggattttttg aatgccatag atggaataca ttcgagtagc ataagtaaag
     3721 ttgattttta cacctctcat gaagcgttgc atttacatta tgagcaagca ttaacaagac
     3781 agttgaaaga tggaaaatgg tacaatcttt caactcattt accctggatt ggaatgcgta
     3841 cggcacaaac ggacagtgca catcttgagt ttctacgagg ggtacaaaat ccaataggca
     3901 ttaagatagg cccagcagct acacctgaat ggttatcgga ggtattaagc gtagccaatc
     3961 cgcaaaaaga agaaggacgg gttttacttt atactcgcct gggagcaaaa cttatcgacc
     4021 gattgttacc tccactgatt gacacagtaa ggaaaagcaa aattccagtc acgtggtcat
     4081 gtgaccctat gcatggcaat accgaaacaa cagaagacgg tactaaaaca cgtcactttg
     4141 ataacatttt atcggaatta aaacaagctt tggaaattca tcgcagcatg ggtagctacc
     4201 tcggaggtgt tcattttgag ctaactggtg acaatgtaac agagtgtatc gggggcgctc
     4261 gtggattagc tcctcatgac ctcaaaaccg cctatcacag cctggttgat ccaagattaa
     4321 actatgaaca atctctggaa atggccatcc aattaagcca tcaattcaga agtgaacctt
     4381 aatgaaactc ataacgtctt gaatgaaggc cagtatctac gccaaaagcc gattgtctgt
     4441 aactgttcac agggtatgta attaaggaat attaagcgcc ctgtctttcc cgcgcaggcg
     4501 gaaatctatc cataaagcta gcacatagat tgatttttag ttagattccc gcctgcgcgc
     4561 actactgtcc ggtaaaggaa gaaatagttg agcgtccttg attaagcgct aagatagcgc
     4621 cctaaatccc catttaggtc aaggacgtac agtgaaagat accgtttttc aagaaataat
     4681 aaaaccaata actaccgatt tacttaaaga atgtgtgact attttcaaat ctgattatga
     4741 ttatgaaaaa tttaagacct atgagcattt acaatcgatg ctgtatgttc atctcaatca
     4801 aataagtagt ttgagaacat tagaaacggc aattaatagt caagatttag ggctatcggc
     4861 gaagatatgt cgttctactt taagcgatgc gaatcgaaga cgaaaagccg attgtttttt
     4921 atggatactg gaacaattac tggagatgtt acctaaaaag caaaaaaaag aattttcaaa
     4981 aatagttcga gttttagata gcagtccaat tcaactcaaa ggatatggtt atgaatgggc
     5041 aaaacacaat gcaacacgcc gatgtgaagg gctaaaattg catgttgaat atgatttagg
     5101 attagaatca ccaacacgag ttgcgttaag ctttccaaat tttaatgatt cgtcaatggg
     5161 taaacaatgg ccaatagaaa ccgatatcat ttatgtgttt gataaaggat attgtgatta
     5221 tgattggtgg tggagtatcc atcaaaaaaa agcgtttttc gtttctagat taaaagttaa
     5281 tgctgctatt agcatagagc agaaatttga aacaaatgaa aactctccta ttcttgaaga
     5341 tggcttattt cgattttcca atcctaagcc tagaggtggc aaaaaaaatt tatatacttc
     5401 tcttgcaaga cgaattagtg ttcagcgcga ggataaagac cctcttattc ttgtgactaa
     5461 tcttcttgat gaaccagccg aaatgattgc ccaattatac aaatccagat gggaaattga
     5521 gctttttttt aaatggataa agcaacgatt aaaaataaaa aaatttcttg gaaagtctga
     5581 aaatgctgta aaaatccaac tgattaccgc aattattgca tatcttctcg tatttttatt
     5641 taaaaatagt cacaatcaaa caacaccact ttacttgatg ctcgtttgga tcaaatgcaa
     5701 cttagaaaaa ccagtactat ttaatgcttt ctatttagat aaaaagccta tgtctccaga
     5761 agttaaatat ctactggaga ctaaaactta aaccggacag tagtgcgcct gcgcgggaaa
     5821 gacaactcaa ataaataggc ttataaacag aaaaacttgc ataccccgtg tacagttacg
     5881 attgtcttat tcttgaggca tttcactatt ttgaaacaat aatttgcctc gcacaggcta
     5941 caaatgatat tgaggactta tttcatgcac cgcctcaatc attattgcaa cattctcagg
     6001 gggtacatcc ggagtaatgc catggcccaa attaaagata tgccctgttc cataaccata
     6061 agatgccagt acactcccaa cctctcttcg tatgcaattt tttgtagtta gtaaaacagc
     6121 aggatccaga tttccttgta aagcaacctt tccgcctact ctcttgcgcg cggaagccaa
     6181 atcacacgtc cagtcaactc ctaatgcatc acaaccagtt tctgccatcc attctaacca
     6241 ttgcccacca ttcttggtaa ataaaattac ggggatatcg gggtattctt ttttcaattg
     6301 ctgaacaatt tgatgcatat atgccaaaga aaaatcctta taattttgtg gagttaaaac
     6361 tcccccccac gtatcaaaga tcataaccgc attaaccccc gcctttattt gctcaattaa
     6421 ataggcagtc acagagacag ccaatttatt caataacagg tgcgctgctt ctttttcagt
     6481 gtatattaaa tttaaaattc gcttaaaatc tctactgctc ccgccctcaa ccatgtaaca
     6541 agctaaagtc catggactac cagaaaatcc gatcaaaggt aattctttag gcatttcctg
     6601 gcggattaag cgcgcagcat caaagacata ggataaagat tcaggaatgg aaggaatttt
     6661 cagggtatga attgccttgg tatcttgtaa aggatttgtg aaacaaggtc cttctccttc
     6721 ggcaaaatag agtcctagcc ccatagcatc aggtattgtt aaaatatcgg aaaataaaat
     6781 agctgcatcc aaagcatagc gacgcaacgg ttgcaaggtt acttcacagg cgagttccgg
     6841 attcttgcaa agatttaaaa aatcaccagc atgctctctt acttttctat attcaggcaa
     6901 ataacgccca gcctggcgca taatccagat tggagttctc tccacaggtt gtcgtctcaa
     6961 agctcgtaga aaaagggatt ggttcaaatc aaacatcatt taatcactat cacaataaaa
     7021 agtgtttata gtatcatgtt aaggtaatca atacagtggt ataaatagca atcgatactt
     7081 aacaatttaa acaggacaca cgaaaaactc caaggtcaag gcaaacaata tttacataag
     7141 aaagtttaga taaattaaat gactgagtta aaaaatttta aacaaaaaga ttgaggattt
     7201 ttcgtaagtc ctgataaatt attaatggat atcaaatgga tactttaaat attacagctc
     7261 tcagtgttga aacaaaaatt ggtgtttatg ggtgggagca gcgtattaat caacgattat
     7321 taattgatat atccattccc tttgacttta aagattgcaa agataatttg tctaatacac
     7381 tggattacgc tgatttgtgc caaacagtaa ctcaatttgt agagtcaact tcgtttcaat
     7441 taattgaaac ggtagcggaa caaattgcac aattaatcaa gcaaaagtat cctgttagta
     7501 aggtttccgt cgctgttaca aaaccccatg ctgtaaaaaa cgctgcggga atccaagtag
     7561 ttgtaaacag ataatctgaa agaagcaatc ttctttgatt aaatgattac tccttatccc
     7621 ccaacgctgc gcaccatgcc gttcaggtgc tctgttttgg caaggttcat ttgagctttt
     7681 tgagccaagg ccttatttgg ataggggccc accactaccc tgaaccaatt tccctgtgct
     7741 tgggtgactg gaaccacgct gacatcaaaa cccttaagga ttagcattcc tttcatttgt
     7801 tctgcatcct gacgcgcttt aaaagatgcg acttgaacca aatatgctcc tttattaggt
     7861 tgttgcatgg ttgttggttt tgcctccgct acctttactg gggtttgtgg tgttggatta
     7921 gctatcgttg atttcactga attatttaca gcagcagtga cagtatttgc agtcttttgt
     7981 attggagttg atgtcttatc tgccgcatga ttgtctgtat tttgctgact agatgcctga
     8041 gatgccggcc ccctttcatt cgctaataat gtataaaact caaatttagg cttaggaggc
     8101 acttgtttat gttcttgcgc cactttagtt ggcgcttttt catcatcatg ttgagctaaa
     8161 acctgtgtat tcacccattg acttatcgtt tgtacatcga gaaacgaagc ggttatatat
     8221 ccaaacaaaa aagtcacaat aataaccaaa aactggtgcg gtcctctgtt gcgacgtgca
     8281 gcacgcctat ttccataatc ttttgccatc acatactctc aggagctgat acgcctaata
     8341 tagccaaacc attatttaaa acctgtcgaa ccgactccag caaacacaaa cgggcacatc
     8401 gcaattgttc ttgttcgcat aatagctgga tcgcattata ataactgtgt aacccattgg
     8461 ctagctctct taaatagtaa gcaagctgat gcggctcgca agaagcagcg gcagattgaa
     8521 tgacttcggg atatcgggca atcagagaaa ttaatgtggt ctcatgttgt tgctccaata
     8581 aatcaaggtt tttaagtccc atatccttat cccacttcaa acccctttct tttaactgcc
     8641 tcaatacact gcagatacgg gcatgtgcat attgaatata atacacagga ttgtcactgg
     8701 attcggactt tgccaggtcc aggtcaaaat ccatgtgttg ttcaggtttt cttgccacat
     8761 aaaaataacg agccgcatca tttcctactt cttctcttag ctctctcaaa gtaacaaatg
     8821 aaccactgcg agtagacatt tgtaccctgt caccacctcg atacagaatg gcaaactgca
     8881 ccaaaatgac atccaaagca ctttcatcat gtcctaatgc cttaacagct gttttaattc
     8941 tcgtgacata accatgatga tcagcaccaa aaatatcaat gactcgatca aaacctctat
     9001 catatttatt ccagtgataa gcaacatcag aagcgaaata tgtcgtttgc ccattagccc
     9061 ggactaatac tctatctttt tcatcaccaa aatcagttgc cctaaaccat aaagcgcctt
     9121 ctctttcata agtgtggcct ctatctttca aggcctgaat acctttctca atagaaccat
     9181 cttcaaataa cgattgctca gaaaaccaac tgtcaaaacg aacaccaaac gcctgcaaat
     9241 catcttttat gtcatccaat accgttttta aagcatgttg atggaagtta gcaaacccat
     9301 ccttgcccag taaggattgc gctcgcgcga tgatcgcatc gatataagtc tctttatctc
     9361 caccttcggg ttcatcagca ggtaaatttt ctactaccga aatccatgga tgcacgaatt
     9421 gattaccctg ctctgaccac atctcttgtg caatctcata aacatattga cctttatagc
     9481 cattcgccgg aaaaacaatt ggctcacctg ccaattccag gtagcgcagc cacacactca
     9541 cagcgagaat attcatttgc ctgcccgcat cattgacata atattccaaa gtcacatcgt
     9601 aaccagcagc ttttaaaaca ttacccaaag ttgctccgaa agcagctcct cttccatgtc
     9661 ctacatgtaa aggccctgtt ggattagcag aaacaaattc aatcaatact ttctggctct
     9721 gtcctaaatt acctcggcca aactccttgc ctttattcaa aatttcagaa atgatgagcg
     9781 agcgtgccgt gttgcgcatg aaaaaattaa taaatcctgc cccggctatc tcaattttct
     9841 ctacagacgg atccgagggg attactgcaa ccaatcgctc ggctaatact ttaggtgctt
     9901 gtcggcatgg tttagctaat accagtgcca aattagtcgc ataatcacca tgagctggat
     9961 ctttggcatt ctcaactttt atttcaatct ccaaatcaat tggcatttcg cctgattgtt
    10021 gcagattaat taatgcctgt ttcaaaagat attcgattat agctttcata cttctcattt
    10081 atccgtcaaa aataccgata ttatgctctt tttcatacca tatggaaagg ctctcaaccc
    10141 agaagcacca tcactgcaac aatagaaaca gcaatagctc attaattttc aagagattca
    10201 taggatttgt gaatatatcc atcttgagac agcagaattg ctatgggtct tgtcacatca
    10261 aagtgcgcaa aaataatcat catcattaca gtgataggca cagacaaaaa catgcctagt
    10321 accccccaaa tggcacccca tagcgctaag gcaaacaata tcactaaagg gcttaagttt
    10381 agtgatttac ctaatagacg aggctctaga aaattgccca cgaaaaactg tatagccact
    10441 atcccggaag tcacttccaa aaacggccac cagctttcaa attggatcaa agcaagcgca
    10501 gctggaaaag ctgttgctac aatagctcct atattgggga tataatttaa aaagaaaatt
    10561 aataatgccc aaaactcggc aaaatccaat ccaacccatt tcataataaa ccaactcaaa
    10621 gtggcagtga gcaggctcaa caaagtttta atacccagat aagtttgagt atcttttgta
    10681 atatgggaga taatgttttt aaccagcttc ctgtgctctg cctgagggaa aaaggcatcc
    10741 atcttttgag taaaataatg ctgctcgaca aacagaaaca caacatataa ggaaatcagt
    10801 acagcggaac tggttattgt cgtaaaaacc ccataaatat taacaagcat actctgaatg
    10861 cttaaccctt gaattaaact gtcgaaatca aaaataccct taacatgaaa tcgttgatcc
    10921 aggctgttaa atatgtgcag taaattttcc tgatacctgg gagaagcggc tatcacatca
    10981 ctgacattat tggtaatgat gttaataaga accattacga ggcaaacaac aacaaacaaa
    11041 gataaaatca tccttatcca ggatgggaga tgtgtgccga tgaaaggaat tttctgaaca
    11101 ccgctattta tggtattcaa caggtgccaa acaaaaatgg atataacaat tggaattaat
    11161 agtcctcttc ctgcgattaa taaataacct gttatccaaa ccagtatcaa acccgctgtg
    11221 aacgttataa tacgattcat ccaattacac tccatctttg atgaatagat actcgcaagc
    11281 aacaagtaac ttagtaatga tttgggcaaa attgccagaa tgatgactca agtcgcaacg
    11341 aatatatttt cctgttatta ttaaattttt aatcttaaat aataccgtgc tgagtaactc
    11401 ttgtgaatat ttaactggcg tcggcccaac cattgcagca aaacttgcta aatgtggaat
    11461 tttcacgata caagacttgc ttttccacct gccttataaa tatcaagaca gaaccaggat
    11521 aacaccaatt caggatttac gctccaatga atggtgcgtt atcgcggggc atgtatgcaa
    11581 aactgaaata aaatacggca aaaggatgat gttgaattgt tatgtggaag ataaaacagg
    11641 aatagttaag cttcgttttt ttcatttcaa taagcagcaa attcaggctt tgaataatag
    11701 cgccatgatt cgagcttttg gcgaagtccg cggatttaac aaccaattgg agatgattca
    11761 tccagaatat caattaattg accaagaatc agatttccac gtggaagaaa cgcttacgcc
    11821 aatttatcca tcgactcaag gcttgactca aacccgatta aggcagttag ttaaaatagc
    11881 cctggaacaa tctgaacacg aacttcatca attggaatgg atgagtgaaa aacaattaca
    11941 ggaaaacaat ttttatgatt tgggcgaagc cattaaatta ctacataacc ctccaccaga
    12001 tatctccctc tccaacctcg aagccggcga acatcctgct ctcaagcggt taatatttga
    12061 tgaattactg gcacagcagc tcagtatgca atttgcaaga cagtcacgca gcaaactcca
    12121 agctcccgct atacttttcg ataatgcaat tcataaacgt tttatcgaat cattgccatt
    12181 cagtcttaca aacgcacaac aacgggtttt taaagaaatc agcgttgatt taactcaaag
    12241 caaacccatg cttcgtttgc tgcaaggaga tgttggggcg ggtaaaacca ttatcgcggc
    12301 actagccgcc cttcaagcca tttcacaagg atttcaggtt gcttttatgg caccaaccga
    12361 tttattaagt gaacaacata caaatagcct cagtaaatgg ctagaaccta tcggtattaa
    12421 tgtattaaga ctgagcggta aaatgaaaac cacagaacga aaaaatgcgc ttgcagccct
    12481 gcaggacaat agctgtcagc tcattgttgg aactcatgcc ttatttcagg agcaagtcga
    12541 attcgctcgt ttgggtttgg tcattattga cgaacaacac cgatttggag tagaacaacg
    12601 gctccttctg caacaaaaag ggcaattaaa ccaattaatc cctcatcaat tattaatgac
    12661 agccacccct attccaagaa ccctgtcaat gtctcatttt gctcatttgg atatatcggt
    12721 catcgatgaa ctaccacctg gccgtatgcc aattacaaca gcggtattga accaggataa
    12781 gcgtgaactc attatcgaac gtttgcaagc agcaatcgcc aacggcaaac aagcttactg
    12841 ggtctgtacc cttatagaag aatctgaaaa attacaatgc atagcagcaa cagatacctc
    12901 aaaaaaatta caagaacagc tctcttgcgc gcgtgttggt cttgttcatg ggagaatgaa
    12961 agcgcttgaa aaagaagcta ctatggcggc ctttaaacaa ggcgagattg atctattggt
    13021 ggcaactaca gttattgaag ttggtgtaga tgttcctaat gccagtttaa tgatcattga
    13081 aaatgcggag cgacttggtt tgtcgcagtt acatcaatta cgcgggcgcg tcggcagagg
    13141 taataatcaa tctcattgtc tattacttta tcaatctcct ctatcacagc aaggtgctga
    13201 acgtctcaag atcatgcgtt caacaacaga cggttttcta atctccgaaa aggatttgga
    13261 gttaagaggc tcaggagaaa tattaggaac cagacagact ggctttagac aatttaaaat
    13321 agctaactta caacgcgata aaaccctctt cgctatttta cgtccaattg caaaacaact
    13381 gatgctggaa aaaacagcaa tcgccagaca aattacacaa cgatggctgg gcaattttga
    13441 acaatttttg caaagttaaa aaaataatta aaattattga agtcaagact gttttttatt
    13501 tttttctttg ctaacataag gatgccagaa ataaaattca ttccctctta tccctgtgga
    13561 taactttaaa aatcaattgc acaaaactct ttccaatttt actattgaaa tttctatagc
    13621 cctttccatg taagggttac tcggcttttt tgatttaatt cactcaaata tatttatcca
    13681 caaaatctat ggataatcct gtgcacagaa tgcaaaacac cttgtaaaat cagtcgattt
    13741 tctaagtgat tcagatcttt ccaatttgct tcctagtgaa atcgacaaat aatttttctc
    13801 atcgctctat aggttttaag ccta
//
LOCUS       DATNSN010000089        13781 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.089, whole genome shotgun sequence.
ACCESSION   DATNSN010000089 DATNSN010000000
VERSION     DATNSN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.089"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..475)
                     /locus_tag="VAH76_002013"
     CDS             complement(<1..475)
                     /locus_tag="VAH76_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386622.1"
                     /translation="MVAQRHLYIFTLIGLLLGVTVDILIRYNNTTAFIYSVVTIFGVL
                     FALTYNNVNLSRLIGTSFLLAFFLSIPLFPLKMDYSTKDYFHFFTFFVGFPFFIYVAH
                     CFHYAYHHDNTWRVSYSSLFAGVWNTIPLLFIAFVFSSLANLLIALGSFVFKTVGN"
     gene            complement(636..1721)
                     /locus_tag="VAH76_002014"
     CDS             complement(636..1721)
                     /locus_tag="VAH76_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEO1386623.1"
                     /translation="MKNLYIDPDITKASTISSEFYTSTEWFEITKEKIFSKTWQFCIS
                     TEELTTNRQLIPFTLLPGFLEEPLLFTKDESGTIRCLSNVCTHRGKILIEEPCVAHKI
                     KCSYHGRRFNFCGELLHMPEFELTKDFPSSNDNLSQISTDILEPFLFASLSPIVPFHE
                     VFAEIKERLFWLPMEKMRLDKERCKDYVVKSHWALYCENYLEALHIPFVHPSLRKVID
                     CNTYTTELFRYCNLQLALANPEEASFDLPKGAQDYGKQVAAYYFWIFPNTMLNFYPWG
                     CSVNVVKPLSPELTQVSFLSFVLDESKLGKGAGGALDQVEQEDERVVESVQRGIKSRY
                     YDMGRFSPTKEQGTHHFQRLLCEFLND"
     gene            1929..2903
                     /locus_tag="VAH76_002015"
     CDS             1929..2903
                     /locus_tag="VAH76_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HEO1386624.1"
                     /translation="MQYSRFLCLLNGMLLSSNVAAVSPSAPIFSPYVDLTINTHWDSQ
                     SQDMEPMDLISPAKKLGIKAYHLAFITDSGQCQPAWGAQQNYSVAKGWGKKQFDTLSR
                     EGVKLTASFGGASGTDISYHCDKSQLINTFNQVVNQYHVNVLDFDIENGTANIPNLLQ
                     ALKLFQKEHPDILLSFTLPVMPEGLTSVGKEIITSAATLDLHFNVNIMAMDYGPAYSG
                     DMGDYAISAATNLHQFLQEIYPDKKPEALWQMIEVTPMIGVNDVNTEQFTLSNAAQLK
                     QFAQKNLLGGLSMWSFNRDKPCADKWASPVCSGNNLQSHDYEFVKYFQ"
     gene            3245..5017
                     /locus_tag="VAH76_002016"
     CDS             3245..5017
                     /locus_tag="VAH76_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine NTPase"
                     /protein_id="HEO1386625.1"
                     /translation="MKSKEEWFNEWRQEKAKSEQAYIKTVPLVEEYIKSFDTLAELRS
                     DVANPDSEKIKKMDAHKRRTLSVLLYAKLKLDEVIEKISDSDSQEKDLLVELNNTLNK
                     VYDNPKSDAKKRVKILIETTEHLLDIKNHSDPQLAKELCADLYALLCIAKRSLKKNEI
                     LASWFIDVHEINKKLEELLAKTEKKVESLEEILSERPNKSLDTSLKDRTIQDYFNERF
                     TEILSGDEEQRAKLDKLKVSLKEVADGLSGIVELRKEKATISEKIKKIEALLKIVEEN
                     DKKVAGRQYFLDLLASYKDHFNTLMSTEGGELKKQLAGIIEQLKTPDFYQTVSSTLLW
                     GASWATSLGTVIYRQLTPQAVQDRVSAYAPATLDSEAKALLQELARQQLIELKKQQSL
                     KRDELVQRYSQLASGSPELEKLIINATSDHLEQIAAATTETSKVLDRYDQIEIKLKDN
                     VEKLKGNQLTRKQLSDFIEKNDTWIVKLSNWLAENIHEIFKSDTARMIDKARKAQQKL
                     GEFERQYQTEIESEKAKIENNDSLSSEMKVFLKNQFEETKKAGGMEQKPQKNLSKETL
                     QKLFVSLLPEHEEISSGVIFSPFN"
     gene            5038..6633
                     /gene="legA2"
                     /locus_tag="VAH76_002017"
     CDS             5038..6633
                     /gene="legA2"
                     /locus_tag="VAH76_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegA2"
                     /protein_id="HEO1386626.1"
                     /translation="MSQSVDQSQVIDRLNRYLKWHNMPVSMNEEGICNGLVTVYAKYV
                     LEGKEKRFFELLDQIVRMEADSAIESEVNQFVYDVVLTLFPHQFNKKLSQANSMCALT
                     INNKPLVSSFDFAIATQDKNWEEIFKNLALQEDEIARVSGTAHAIAVRRKNNKYIVYD
                     PNYSSGTKEFSNEHELISELHYNVFGYHNGFYIKGGLLGMMVSVIRHPDNKKPRAFPQ
                     VTHLYDRYLTENNINDVAVSFHGGTFNTLAHAAMVSHAEVIKNLLEKGAKDNDHQAAG
                     NAVMYNNVDALKALLGKNRETELFQRLFHAALESGREEVYDALFSMKGALPFNSSVQA
                     IRAAAKGGNSVLLAKVMNYFKRNHTGFSDLEDAIPSAIASGSVQCIKLLVNQLNESKK
                     PLDDKKRMGYLLESIKQNQSYMVAYFLKDIPPEHLKTIYMSVSAVERTNLHILRQLQN
                     YGVPFSDMSKAVFEQKEHHFVGLRRILGVFLCKFTDFLKELLFNDCGIHCHRAEESGV
                     KKSFFAFPKKLVGVEQLQIPKLY"
     gene            6712..7635
                     /locus_tag="VAH76_002018"
     CDS             6712..7635
                     /locus_tag="VAH76_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEO1386627.1"
                     /translation="MNILLAGGSGLIGQELVRGMGEEHHFTVLGRDKDAIQKIFTTKI
                     SSLSWDELNTQDASRFDAVINLCGVNIAISRWTPKIKEEIISSRVQTTENLLHWILQQ
                     NAKPRFYCANAVGIYGLQENGDATDLTEDTAIHWEHPKDYLSEVGIRWQQALQKAEEA
                     GLPVTSTRFGVVLKKGKGFLGKLYPSFICGLGSTIGDGRQYLSWIDSEDLVNAYRFLL
                     NHSEITGPVNVTSPNPCMQREFAKAFAKSLHRPLLLWTPAFIIHLLFGEMGDCLINHG
                     QKVKPKRLLEAGFEFKWPDIEKALKHQYSAI"
     gene            complement(7732..8148)
                     /locus_tag="VAH76_002019"
     CDS             complement(7732..8148)
                     /locus_tag="VAH76_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HEO1386628.1"
                     /translation="MMFQSKLATFFGALILAGSAFASQQGACPDINVIKAEGLPMAEE
                     IGMNYYLAYNISDYSTSSVWGFFIAPIEADSEEGALEASNEVLSMMNAPGVPHQQDED
                     IICSYDTGTPNLIAAAIKDGSQISPMKLKQYFKSAR"
     gene            complement(8425..9699)
                     /locus_tag="VAH76_002020"
     CDS             complement(8425..9699)
                     /locus_tag="VAH76_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine aminohydrolase"
                     /protein_id="HEO1386629.1"
                     /translation="MAKDKGFFKKAKTGKNKSSATPYTEECAIQIPSEIDNEQMHRMP
                     AGGEEDQYSRIKHMSALIKKYGNLPIITTQETSLPDYWLDLFAAIDEGDIPKAHTLFH
                     LLPQDDIILRALRAVHSEDYLYQLIKYCIQAKHFGFKQLNADLVVTPKTFEILIKDCA
                     ATLFNPAKTHFSFGLPSHHAYTQMGSGFCLINKTAMLIKQAELSSVQPPRFVIIGTDV
                     NRDNGLCDILRHSFSHLSICHIDVFDSRVYPQQDFSYINNEFNSEGVDVGKNIHVWHH
                     NDLSYYAVDLSLTSRKSVGVHPALLFALEQFKASIREAKAKGQKIALYLPTGWDSHED
                     ETAYCGKFVKGRMLGKTAAHQFRFNDGDLSYFYESIFTLYNENKDCIDTIYWGLEGGY
                     DRTMYERELTLLLQVIEKQLLPQDSNSHNMSC"
     gene            9899..10303
                     /locus_tag="VAH76_002021"
     CDS             9899..10303
                     /locus_tag="VAH76_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEO1386630.1"
                     /translation="MVQHDIIQLPPKPYGAHLITNEVMQKITLRGTGLLHLFIQHTSA
                     ALALNENASPDVLIDLNHFFTQLVPEHQHYRHDIEGPDDMPAHIKSILCGSSLTIPFQ
                     ENKLLLGVWQGIYLMEFRKQAGARTIVASQLI"
     gene            10439..11488
                     /locus_tag="VAH76_002022"
     CDS             10439..11488
                     /locus_tag="VAH76_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEO1386631.1"
                     /translation="MTNDHYLSKKKMFAVVFCVFAPNEVLLANDTAVGGEGSLPIPVS
                     QPHIKMVSELIRISGKNLNEPQMNGSWHYNCNFTFKNTLNKEVTISMAFPFPINDGNS
                     EIALPAGQQTNVGQALVYDFLVTVNDKQVPAQHGNIAPDQTKGLYYEDAYFWKTTFPP
                     LATVNIHHDYSTGATYDVMGYHWVRYVLKTGALWQDSSIGHTRLEVIPNTPTRLCSEI
                     DPKADYLNPTPSGMSISGSRADRKYIWDLRHFQPQADLSLCLFTGISYVRYKVIYPWL
                     NSDDALGKLARLSNKELRFLRNTIYAQYGRQFQSPDLQEYFSKKWWYVPNPDYSDRML
                     NEEDKKLLSMINQAK"
     gene            complement(11649..12176)
                     /locus_tag="VAH76_002023"
     CDS             complement(11649..12176)
                     /locus_tag="VAH76_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEO1386632.1"
                     /translation="MTIFKNLVLTLVSSALLVTPCFSAGLTLESPAFASNAVIPQKFT
                     CSGLNYSPPLIWHDPNLGTQSYVLIVHDPDAPTGNWIHWVLFNIPAQVKQLAERTATP
                     AGATSGLNSWNTTGYSGPCPPAGTHRYYFTLYALDTYLNLGPEATAQDVVNAMKGDII
                     DTAELVGHYSKNASQ"
     gene            12496..13509
                     /locus_tag="VAH76_002024"
     CDS             12496..13509
                     /locus_tag="VAH76_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO1386633.1"
                     /translation="MKAIGYYHSLPITDNKALMDLDVSVPKATGHDILVEIKAVAVNP
                     VDTKVRRNVQPEPQQPKILGWDASGVVTEVGSEVTMFKPGDEVWYAGDLTRSGSNCEF
                     HLVDERIAAKKPKSLSFQEAAALPLTSLTAWELLFDRLQLSAEDKGILLITGAAGGVG
                     SILVQLARQLTQLTIIGTGARPESIQWILDNGAHHVIDHTKDMKAQLQNLHYSEVDYV
                     ISLTHTDQHAKSLVEVLKPQGKFALIDDPQDLDIKLFKLKSISIHWEMMYTRSMFKTA
                     DMIKQHDILTEVARLVDAGKIKTTIAECLGLINADNLRRAHALLESGTSRGKIVLNGF
                     KSI"
BASE COUNT         4432 a         2371 c         2815 g         4163 t
ORIGIN      
        1 tatttccaac agttttaaat acaaaagatc ccaatgcaat aagcaaattg gccaatgagg
       61 aaaagacgaa ggcaataaac aaaagaggaa ttgtattcca taccccagca aataaactgc
      121 tatagcttac ccgccaggtg ttgtcatgat ggtaggcgta atggaaacaa tgagcgacgt
      181 agataaaaaa tggaaagcca acaaaaaaag taaaaaaatg gaaatagtct ttcgtagagt
      241 aatccatttt caacggaaac aatggtattg atagaaagaa agcgagcagg aagctggttc
      301 caatcagacg cgacaaattg acgttgttat aagtaagggc aaataaaaca ccaaagatag
      361 tgacaacact gtatataaat gcagtcgtgt tgttgtagcg tatcagtatg tccactgtta
      421 cccctagaag taatccaatc agggtaaaaa tataaaggtg tctttgagca accatcctgt
      481 ctttctccac aagggggtta tattcatgat tcagggttta gtagaacgcc ctaatcgtta
      541 tctaaaaata attctaactc agttacttaa ttgatctaaa tcgtttatta aaaatatttt
      601 ttcgttccaa gtctgagcat gtgacagatc taatttcaat cattaagaaa ctcacacagt
      661 agtctttgaa aatggtgtgt accctgttct tttgttggag agaaacgtcc catatcataa
      721 tagcgagatt ttatccctct ttgcacagac tccaccactc tttcatcttc ttgttcaact
      781 tggtcaagtg ctccaccagc gcctttccca agttttgatt catcaagaac aaaggataaa
      841 aaggaaacct gagttaactc aggactaagc ggtttgacta cattcacaga acaaccccag
      901 ggataaaaat taagcatcgt attggggaat atccaaaaat aataggcagc aacctgtttg
      961 ccataatctt gtgctccttt gggtaaatca aagctggctt cctcagggtt tgccaaagcc
     1021 aattggagat tacagtagcg gaagagttct gtggtgtagg tgttgcaatc gattactttt
     1081 cttagcgagg gatgtacaaa agggatatgt aacgcttcca ggtaattttc acagtataaa
     1141 gcccaatgcg atttaactac ataatctttg catctttctt tatccaggcg cattttttcc
     1201 ataggcagcc aaaacaatct ttctttaatt tctgcaaata cttcatggaa cgggacgatg
     1261 ggggaaagtg aagcaaagag aaaaggttca agaatatccg ttgaaatttg acttaggttg
     1321 tcattgctcg atgggaaatc ttttgtgagt tcaaattcag gcatatgaag taattcgcca
     1381 caaaaattaa accgacggcc atgataagag cacttgattt tatgagctac gcaaggttct
     1441 tcgatcagga ttttacctcg atgggtacat acattactca aacaacgaat tgtacccgat
     1501 tcatcttttg taaacagcaa gggttcttca agaaatccag gcagcagggt aaaaggaatt
     1561 aattgtctat tcgttgtcag ctcttcggta ctgatacaaa attgccaggt tttactaaat
     1621 atcttttctt ttgtaatttc aaaccattct gttgatgtat aaaattcaga agagattgtg
     1681 gaggcttttg taatatcagg atctatatag agattcttca tgaacatttc cttactgtaa
     1741 agagaaaaac ctgcaaaaag agaataaaca ctataaccat ttcttttggt gaagcgatgg
     1801 ttaaggtttg gtaaaaggta gtgcaaacct ttgtaccctg tcaacttgtt tatttgataa
     1861 caaaatgatt gattaattgt cctaatgatg caaaaataaa cacagtttgg gttttaagga
     1921 actattgaat gcaatatagc cggtttttat gtttattaaa tggcatgtta ttatccagta
     1981 atgttgcggc ggtttcaccc tctgctccta tctttagtcc atatgttgat ttaacgatta
     2041 atactcattg ggattcgcaa tcacaggata tggagccaat ggatttgatt tctccggcaa
     2101 aaaaactggg gattaaagct tatcatttgg cttttattac agacagtggt caatgccaac
     2161 ctgcctgggg agcccagcaa aattatagtg tggcaaaggg atggggtaaa aaacagttcg
     2221 acacgttatc aagagagggg gtgaaactca ccgcttcatt tggcggagcg agcggtactg
     2281 atatttccta tcattgtgat aaaagtcaat taattaatac gtttaatcaa gtggttaatc
     2341 aatatcatgt gaatgtcctt gattttgata tagaaaatgg gacagccaat attcctaatt
     2401 tgttgcaggc tttgaaatta ttccaaaagg aacatccgga tatcttgtta agttttactc
     2461 ttcctgtgat gcctgagggt ttaacttctg ttggaaaaga gataatcacc tcagcggcta
     2521 ctctagatct tcattttaat gtgaacataa tggctatgga ttacgggccg gcttatagtg
     2581 gagatatggg ggattatgcg atatccgcag caacgaatct ccatcaattt ctgcaggaaa
     2641 tttatccgga taaaaaaccg gaggctttat ggcagatgat tgaagtaacg ccaatgatag
     2701 gtgtgaatga tgtgaataca gagcaattta ctttatctaa tgcagcccaa ttaaaacaat
     2761 ttgcacagaa aaatttgctg gggggattgt caatgtggtc tttcaatcgg gataaacctt
     2821 gtgctgacaa atgggcaagt cctgtatgta gcggtaataa tttgcagagt catgattatg
     2881 aatttgtgaa atattttcag taatggaatt caaagcgcac tggaaaagct ctgaaaattc
     2941 ggaattaact cgggtttctt tagatctgat caataagaaa aatgcttaaa tttaattgca
     3001 aatgacatag ataagttgaa agtaataaca caaaaatggc ttgaatgatt tatctatgtg
     3061 gtttttttgt caatataaac ctggttttga tgaattaggt gatataattt tcaatatgat
     3121 aaattctatg aaactcatta cttaatgctt agataataat agataaatat actggggtta
     3181 agatttagcg gatatttcgc tatttattgt ttggaagagt gctcactggc aattagggtt
     3241 aattatgaag agtaaagaag aatggtttaa tgaatggcgc caagaaaagg cgaaatcaga
     3301 gcaggcatat attaaaaccg ttccattagt agaggaatac attaaatcct ttgatacctt
     3361 ggctgagctc agaagtgatg tggctaatcc tgattctgaa aaaattaaaa aaatggatgc
     3421 tcataaaagg cgtactctga gtgttttact ctatgccaaa ttaaaactgg atgaagtgat
     3481 agaaaaaatt tcggattctg atagtcagga aaaggattta ttagttgaat taaataatac
     3541 tctgaacaaa gtgtatgata atcccaagtc ggacgccaaa aagagagtga aaattttaat
     3601 agaaacgaca gaacacttat tggatatcaa aaatcactct gatccacaat tggctaaaga
     3661 attatgcgca gatctatatg ctctattatg tattgcgaag agatcactta agaaaaatga
     3721 aatactggca agctggttca tcgatgttca tgaaatcaat aaaaaactgg aagaattatt
     3781 agcaaaaaca gaaaaaaaag tcgaaagtct ggaagaaatt ctgtcagaaa ggcccaataa
     3841 atcacttgat acttcactaa aagacagaac gatacaagat tattttaatg aacgattcac
     3901 cgaaatttta agtggggacg aggagcaaag agcaaaactg gataaattaa aggtgagcct
     3961 taaggaagta gctgatggac tatcaggtat tgtggagtta agaaaagaaa aggccacgat
     4021 tagtgaaaaa attaaaaaaa ttgaagcctt actgaaaata gtagaagaaa atgataaaaa
     4081 agtagcagga aggcaatatt ttttagattt gctagccagc tataaagacc attttaatac
     4141 tctaatgagt actgagggtg gggaattaaa aaaacagtta gcagggataa tagaacaact
     4201 caagaccccg gatttttatc aaactgtttc ttcgaccctt ttatggggcg caagctgggc
     4261 tacttctctg ggtacagtca tttatcgcca gctaactcct caagcagttc aggatagagt
     4321 tagcgcttat gctcctgcaa cactggatag tgaagcaaaa gctcttttgc aggaattggc
     4381 aagacaacag ttaattgagt taaaaaaaca acagagtctc aaaagggatg aattggttca
     4441 gagatatagc caactagcaa gcggtagccc tgagttggaa aaactgataa tcaacgccac
     4501 ttcagaccat cttgagcaaa ttgctgcagc aacaacagaa acaagcaaag ttcttgatag
     4561 atatgatcaa attgagatta aactcaaaga taatgtagaa aaacttaagg gaaatcagtt
     4621 aactcgaaag caattatctg attttattga aaaaaatgac acctggatag taaaattatc
     4681 caactggctt gcagaaaata tccacgagat atttaagtct gatacggctc gcatgattga
     4741 taaggctaga aaagcgcaac aaaagctggg agaatttgaa agacaatatc aaacggagat
     4801 tgaaagtgaa aaagcaaaaa ttgaaaataa tgattcattg tcctcagaga tgaaagtttt
     4861 tttaaaaaat cagtttgaag aaacaaaaaa agcaggtgga atggagcaga aaccacagaa
     4921 aaatctcagc aaggaaactc ttcagaaatt atttgttagc ctgttgcctg aacatgaaga
     4981 gatctcatct ggggtaatat tcagtccgtt taattaatca gggattagag cgatgatatg
     5041 agtcaatcgg ttgatcaaag tcaagtcatt gatcgattaa atcggtatct caaatggcat
     5101 aatatgccag ttagcatgaa tgaagaagga atttgcaatg gcttggttac cgtgtacgca
     5161 aaatacgtac tcgaaggtaa agaaaagcgc ttttttgaat tgcttgatca gatagtcaga
     5221 atggaagcag actcagcaat agagtctgag gtaaatcagt ttgtctatga tgtcgttctc
     5281 actttattcc cgcatcaatt taataaaaaa ctaagtcagg ctaattccat gtgtgccttg
     5341 acaataaaca acaaaccctt ggtttcttct tttgattttg caattgcaac tcaagataaa
     5401 aattgggagg aaatctttaa aaatcttgct ctgcaagagg atgagattgc tcgtgttagc
     5461 ggaacagcgc atgccatagc cgtcaggaga aaaaataata aatatattgt atacgatcct
     5521 aattactcgt ctggtactaa ggagttctcc aatgaacacg aattgatttc cgagttacat
     5581 tacaatgtat tcgggtatca taatggattt tatattaagg gtggtctttt ggggatgatg
     5641 gttagtgtca tccgccatcc cgacaataag aagccaagag cttttcctca agtgactcac
     5701 ctttatgatc ggtatttaac cgaaaacaat attaatgacg tcgctgtttc atttcatggt
     5761 ggaacattta atactttagc gcatgctgca atggttagtc atgccgaagt aataaaaaat
     5821 ttgcttgaga aaggagcaaa agataatgat catcaggctg ctggaaatgc tgtcatgtac
     5881 aacaatgtgg acgcattaaa agctttactg ggaaaaaata gagagacaga actctttcaa
     5941 cggctatttc acgcggcttt ggagagcggg agagaagaag tttatgatgc tttgttttcc
     6001 atgaaaggtg ccttgccatt taattcgtct gttcaagcca ttcgagctgc agcaaaaggg
     6061 ggtaattctg ttctcttggc caaggtaatg aattatttta aaagaaatca tactggattt
     6121 tcagatcttg aagacgccat tccttctgca attgccagtg gaagtgtcca atgtatcaaa
     6181 ttacttgtta accaattaaa tgaaagtaaa aaacctttag atgataaaaa aaggatgggg
     6241 tatttgctgg agtcaataaa gcaaaatcaa tcttacatgg tggcttattt tcttaaagat
     6301 atcccaccgg aacatttaaa aacaatctat atgagtgtct ctgctgttga gagaacaaat
     6361 ctgcatattt tacgtcagtt acagaattat ggagttccat tttcagatat gtcaaaggct
     6421 gtttttgaac aaaaagagca ccattttgta ggtttaagac ggatactagg tgtttttctg
     6481 tgtaaattca ctgattttct taaagaactc ttattcaatg attgcggaat acattgccat
     6541 cgagcagaag agagtggagt aaaaaaatca ttttttgcct tccctaaaaa actggttgga
     6601 gttgaacaat tacaaatccc taaactctat tgaggcacgg tactaatgac aaacagtact
     6661 catcagttta tattaatcaa ataagatggc ctgaaaggag cttgattcat tgtgaatatc
     6721 ttattggcag gaggctctgg attaatagga caagagttag ttcgtggaat gggagaggag
     6781 caccatttta ctgtgctggg gcgtgataag gacgcaatac aaaagatttt tacaacaaaa
     6841 atatcctctt tgtcctggga tgaattaaat acccaggatg ccagtcggtt tgacgctgta
     6901 attaatttat gtggcgtcaa tattgccatt tcacgatgga caccaaaaat aaaagaagaa
     6961 ataatcagtt cgagagtgca aacaacagaa aatttgcttc attggatttt gcagcagaat
     7021 gccaaacctc gcttttattg cgccaatgca gttgggattt atggtttaca ggaaaatggt
     7081 gacgcaactg atttgactga agatacagca attcattggg aacacccaaa ggattaccta
     7141 agtgaagtgg gaattcgttg gcagcaagcc ttgcaaaagg cagaggaagc gggccttcct
     7201 gtgacttcca cacgctttgg cgttgttcta aaaaagggaa agggtttttt aggaaagctc
     7261 tatcccagtt ttatttgcgg attaggaagt actattggag atggtcgcca atatctttcc
     7321 tggatagaca gtgaggattt ggtcaatgcc tatcggtttt tgttaaatca ttctgaaatt
     7381 accggacctg tgaatgtgac ctcaccaaat ccatgcatgc aaagagaatt tgctaaagct
     7441 tttgccaagt ctctgcatag gccactatta ttgtggactc ctgcttttat aattcatctt
     7501 ttatttggtg aaatgggtga ttgtttaatc aatcacgggc aaaaggtaaa acccaaacga
     7561 cttttggaag ctggttttga gtttaaatgg cctgacattg aaaaggcact aaagcaccag
     7621 tattctgcta tatagtaaca gcatttttaa gaggatttca tgatttggag ggatatttaa
     7681 aataccctgt aagttactga atacttgaca gggtatttaa aggagtacta attaacgagc
     7741 actcttaaag tattgtttta atttcattgg agaaatttga gagccatctt tgatagcagc
     7801 ggcgattaaa ttaggagtgc ctgtgtcata actgcaaatg atgtcttcgt cttgttgatg
     7861 aggaactcca ggagcattca tcatgcttaa cacttcattc gatgcttcca aagcgccttc
     7921 ctcggaatct gcttcaattg gagcaatgaa aaagccccaa acagaagagg tgctgtaatc
     7981 actgatatta taagccagat agtaattcat gccaatttct tcagccatag gcaaaccttc
     8041 cgccttgatt acattgatat ctgggcaagc accttgttga gaagcaaaag cagaaccggc
     8101 gagaattaaa gcgccaaaaa aagtcgctaa tttagattga aacatcatgt tttttactcc
     8161 tgagttattt ttaattccgc gcaaagagta tcatttgcct agaagattta caataatgtt
     8221 gtaatttgag attggagggt aaacagaaaa caaactggcc ttattctcgg aggtatgatc
     8281 attgattact aatgctatcg cccagaatca gcaatcaaca cagtatggta aatcgctaat
     8341 cgtttttgaa attaatagta actatttcat ggccaaaaca gttcgcgcta tcattactaa
     8401 aattaaaatt tgaaatacta accattaaca ggacatatta tgcgagttgg agtcttgagg
     8461 tagcaattgt ttctcaatga cctgcaacag cagagtaagt tctctttcat acatggtcct
     8521 gtcatatcca ccttccaagc cccaatagat ggtgtcaata cagtctttgt tctcgttata
     8581 caaagtgaat atggattcat agaagtagct tagatcaccg tcattaaagc gaaattggtg
     8641 ggcggcagtt tttcccagca tccggccttt aacaaattta ccgcaatacg ctgtttcatc
     8701 ttcatgagaa tcccatccgg tcgggaggta taaagcaatt ttttgccctt ttgctttcgc
     8761 ctctctaatc gatgctttga attgctctaa agcaaaaaga agcgcagggt gcacacctac
     8821 tgattttctg gacgttaagc tgagatccac cgcataataa cttaaatcat tgtggtgcca
     8881 tacatggatg ttttttccta catctactcc ctcagagttg aattcgttat taatgtagga
     8941 aaaatcttgt tggggatata ctcttgaatc aaacacatca atgtggcaaa tcgatagatg
     9001 ggaaaatgag tgtcttaaaa tgtcacatag gccattgtcg cgattgacgt cggttcctat
     9061 aatgacaaat ctaggtggtt gaacagaact caactcagcc tgttttatca gcatggctgt
     9121 tttattgata aggcaaaaac cgcttcccat ttgggtatag gcatgatggc ttggtaaacc
     9181 aaaagaaaaa tgggtttttg ccggattgaa cagagttgct gcacaatcct tgattaaaat
     9241 ttcaaaggtt tttggggtaa cgaccagatc ggcatttagt tgtttaaagc caaaatgttt
     9301 cgcttgaatg caatatttaa tgagttgata cagataatct tctgaatgaa cagctcgtaa
     9361 tgctcgtaaa ataatatcat cttgcggtag cagatgaaaa agcgtatggg cttttggtat
     9421 atctccttca tctatcgctg cgaataagtc tagccaatag tctggtagac tggtttcctg
     9481 agttgtaata attggtaaat tcccatattt ttttatcagt gctgacatgt gttttatgcg
     9541 tgagtattga tcttcttcgc cgcctgctgg catgcggtgc atttgctcat tgtctatttc
     9601 agatgggatt tgaatggcac attcttcagt gtatggagtg gcagaactct tgtttttccc
     9661 cgtttttgct tttttaaaaa atcctttatc tttggccata tccattccta aatagcgtgg
     9721 ttaatttatt atattttaga ataggcgagt gagagatatt aatccagaga tttgttaagc
     9781 tcatgactat caagacaaga ggcgctactt tctttataat gcaaatagag tttatggaca
     9841 gaatttcatt ttgttctgac tcgataattc ctccgttatc caattaagtc gagggtctat
     9901 ggtacaacat gacataatcc aactgcctcc aaaaccttat ggtgctcatc taatcactaa
     9961 tgaagtgatg caaaaaatta cgttgagagg caccggatta ttacatttat ttattcagca
    10021 tacctctgcg gcacttgcct taaatgaaaa tgcttcccct gatgtattga ttgatctaaa
    10081 ccatttcttt actcaacttg ttcctgaaca tcagcattat cgtcatgata tagaggggcc
    10141 agatgatatg cccgcccata ttaaatcaat tttgtgtggc tcatctttaa ctattccatt
    10201 tcaggaaaac aagttgctat tgggtgtttg gcaaggcatt tatttaatgg aatttagaaa
    10261 acaagcagga gcaagaacaa ttgttgccag tcaattgatt taggatcata gggcataact
    10321 ttactcacca ttgaattaat ctgagcaatt catgtatctt tctgtgtttc attcataaga
    10381 aagaaaaacc tcctgttctt aactatactt tatattattt tgcaaccagg gcagtttcat
    10441 gacgaatgat cattacttat ctaaaaaaaa gatgtttgct gtggtcttct gtgtttttgc
    10501 gcccaatgag gtgttattag ctaatgatac tgctgtaggt ggggagggat ctttacccat
    10561 tcctgtttct cagccgcata taaaaatggt aagtgagtta atcaggatca gtggaaagaa
    10621 tttaaatgag cctcagatga atggttcctg gcattataat tgtaatttca cttttaaaaa
    10681 tacattaaat aaagaagtca ctatttcaat ggcttttccc tttcctataa acgatgggaa
    10741 ttcagaaatt gccctaccag cgggacagca gactaatgtt ggtcaagcat tggtttatga
    10801 ttttctggtt acagtcaatg ataagcaagt tccagcacag catggtaata tcgccccgga
    10861 tcagactaaa ggcctttact atgaggatgc ctatttctgg aaaaccacct ttccaccctt
    10921 ggcaacagtc aatattcatc atgattattc gacgggagct acttatgatg tgatggggta
    10981 tcattgggtt cgttatgttt taaaaacagg tgctctttgg caggatagca gtatcggaca
    11041 tacaaggctt gaagtcattc ccaatacacc aacgcgttta tgctctgaga tagatccaaa
    11101 ggcagactac ctcaatccta ccccatcagg gatgagcatt tcaggctcac gagcggatag
    11161 aaaatacatc tgggatttac ggcattttca accccaagca gatttgtcac tttgcttatt
    11221 tacggggatt agttatgttc gctataaggt gatttatcct tggttaaatt cagatgatgc
    11281 attaggcaaa ttagccagat tgagtaataa agaattacga ttcttacgaa atactattta
    11341 tgcgcaatat gggcgccaat ttcaatcacc ggacttgcaa gaatatttca gcaagaaatg
    11401 gtggtatgta ccaaatcctg attattcaga ccgcatgctc aatgaagaag ataagaaatt
    11461 attgtcaatg attaatcagg ctaaataaac ttaactgctg ataaattttt tacaataaat
    11521 cccctatgag gtttctaaat taataaaggc tgattttaca ggtgtctata accacccggg
    11581 cctatttttg ttttatgcca attcttgtat ttgttactgg atcgcgtgag aaaaacattg
    11641 ttgcaatttt attgagatgc attcttacta taatgtccta ctaattcagc cgtatctatg
    11701 atatctcctt tcatggcatt tactacatcc tgagcagtgg cttctgggcc aagattaagg
    11761 taagtgtcta aagcgtagag tgtaaaatag taacggtgtg tacctgcagg cgggcaggga
    11821 ccactgtaac cggtggtatt ccagctgttt aacccactgg ttgcgccagc aggagttgct
    11881 gtcctttcag cgagctgttt cacttgagca gggatattaa acaataccca atgtatccaa
    11941 ttacctgtag gagcatcagg atcatgtact attaacacat aagattgggt tcccaaattg
    12001 ggatcatgcc atattagtgg gggagaataa tttaagccgc tgcatgtgaa tttttgagga
    12061 atgacggcat tcgatgcaaa tgccggactt tccaatgtta atcctgcaga aaaacacggt
    12121 gtaacaagta atgcagaact gactaatgtc aagacgagat ttttaaatat ggtcatcact
    12181 ttccctgttt ctgttgttta cttacctgaa attattgaga gtctagtctt ttatagattc
    12241 ccttaaatta tagattacga ttgatacact tgtgatttgt ttttcatcag gatttacgaa
    12301 aaaccccaat ctctttgttt aaaaaatgtt taattcggac atttaattta tctaaactcc
    12361 ctcattaaaa gcattttttc cttgaaattg ggggtttttg taagtcccat acatatgaga
    12421 aatgccttgt gctgttgttt aaaaaatttt ctaaacctat aatgaaactc cagtattttt
    12481 aaaggaataa tttcaatgaa agcaataggt tactaccact ctttacccat tacagataat
    12541 aaagcattga tggacctgga tgtttctgtt cccaaagcaa ctggccatga tattttagtt
    12601 gaaatcaaag ctgttgcagt gaatcccgta gataccaaag tgcgtcgtaa tgttcaacca
    12661 gaacctcagc agcccaaaat attaggctgg gatgcttccg gggtagttac tgaagttgga
    12721 tcagaagtga ctatgtttaa accaggtgat gaagtgtggt atgccggtga tttaaccaga
    12781 tcagggtcta attgtgaatt tcatcttgtt gacgaacgca tcgcagccaa aaaaccaaag
    12841 tcgttaagtt ttcaagaggc tgcagcattg cctttaacca gtttaacagc ttgggagctt
    12901 ttatttgata gattgcaatt atcagcagaa gataaaggta ttctattaat tactggagcg
    12961 gctggggggg ttggttctat tttggtacaa ctggcaaggc aacttaccca gttaactatc
    13021 atcggaacag gagcccgccc tgaatcaatt cagtggatcc tggataatgg ggcccaccat
    13081 gtcattgatc acactaaaga catgaaggcc caattgcaga atttacatta ttctgaagtg
    13141 gattatgtga tcagcttgac ccataccgat caacatgcca aatcgttagt tgaggtcctg
    13201 aaacctcaag ggaaatttgc tttgatagat gaccctcagg atttggacat taagttattt
    13261 aaactaaaga gtatttctat tcactgggaa atgatgtaca cccgctctat gtttaaaaca
    13321 gctgatatga ttaagcaaca tgatatttta acagaggttg ccagattggt ggatgccggt
    13381 aaaatcaaga caactattgc cgagtgtctg ggattgatca atgctgacaa tttgagaaga
    13441 gcacatgctt tactagaaag tggtacttcc agaggcaaga tcgttttgaa tggattcaag
    13501 tcaatttagt tattctcgaa acagcgacga tgcctggaat aaaacagaga gagtcacttg
    13561 atttttaaat gattctctaa atagcctacg tgcctcagct tgtctgaggc atccagagag
    13621 cgcttggttg gtattgctga caagcagcag tacccggagg atcactcgtt tagtttatct
    13681 aaatccctca ttaaaaacat tttttgcctt ggccttggtg ttttacgcaa atctatttgt
    13741 gttaaaaaaa tatcttttaa ttaaaaattg gacatggtgt t
//
LOCUS       DATNSN010000090        13650 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.090, whole genome shotgun sequence.
ACCESSION   DATNSN010000090 DATNSN010000000
VERSION     DATNSN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13650
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.090"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..410)
                     /gene="folK"
                     /locus_tag="VAH76_002025"
     CDS             complement(<1..410)
                     /gene="folK"
                     /locus_tag="VAH76_002025"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO1386634.1"
                     /translation="MNVCYLSLGSNQKNPERQIRQAIKEIKQIPSTCLIKASRLYWNK
                     AWGFENQQEFCNVVIEIQTTLLPHKLLKWCQKIENRHKRVRRKFWGPRTLDIDIILYG
                     YRLIRTKNLIVPHPCFHLRDFVLVPLRELNSNLK"
     gene            complement(407..1678)
                     /gene="pcnB"
                     /locus_tag="VAH76_002026"
     CDS             complement(407..1678)
                     /gene="pcnB"
                     /locus_tag="VAH76_002026"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEO1386635.1"
                     /translation="MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRD
                     LLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSS
                     DASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKL
                     IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDE
                     ITKLYQCGEAESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDG
                     KPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQ
                     VIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQE
                     VDDVEKNNMVTQLAATTPGRPKKRRKRKIAK"
     gene            1985..2716
                     /locus_tag="VAH76_002027"
     CDS             1985..2716
                     /locus_tag="VAH76_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEO1386636.1"
                     /translation="MDFIDIGLSLTALIILEVVLGIDNLVILSILTEKLPREKRKKAR
                     RWGLTFAWMTRLLLLGSAVFLVKLVKPILTIGSLVFSARDLFLILGGAFLIWKSTDEI
                     HQDVMNEPLMEPVSKNVSSSATFRGVVIQIALLDIIFSLDSVLTAVGLTSHFMVMAVA
                     ITIAIIIMIWASEPVSKFISEHPTIKMLALSYLILIGTVLVADGFEFHVPRGYLYFAM
                     GFSLAVESLNLIKNERVKRKKLKRE"
     gene            2719..3141
                     /gene="hemJ"
                     /locus_tag="VAH76_002028"
     CDS             2719..3141
                     /gene="hemJ"
                     /locus_tag="VAH76_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEO1386637.1"
                     /translation="MLFIKAFHIIALVAWFAGLFYLPRLYVYHAESLDKISVDRFKIM
                     ERRLYYGITWPAALATTFLGLWLFTYNLSYYLKAGWMHMKLALVLLLWVYHLTCGHFL
                     KSFALGKNTRSSRFYRFFNEMPTLLLVGIVLLVVVKPF"
     gene            complement(3138..3314)
                     /locus_tag="VAH76_002029"
     CDS             complement(3138..3314)
                     /locus_tag="VAH76_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEO1386638.1"
                     /translation="MQEYKRYICVICGFIYDEAEGWPEDGIEPGTRWEDVPENWFCPD
                     CGAGKEDFEMVEMD"
     gene            3738..5027
                     /gene="hemL"
                     /locus_tag="VAH76_002030"
     CDS             3738..5027
                     /gene="hemL"
                     /locus_tag="VAH76_002030"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEO1386639.1"
                     /translation="MSRSSDLFHKAQTIIPGGVNSPVRAFKGVGGEPVFFKSGKGAYL
                     TDVDDKQYIDYVGSWGPLILGHCHPKVIEAVDNVLHSGMSFGAPTELEIQLAEKIASL
                     MPSIEKIRMVNSGTEATMTAIRLARGFTNKNKFIKFNGCYHGHSDSLLVKAGSGLLTL
                     GIPSTPGIPQSITEHTLTADFNNLEQVAQLFEKYPNDIATVILEPVPGNMGFILPKIE
                     FLKGLRELCDQYNALLIFDEVMTGFRVGLHGAQGLFGIKPDITTLGKIIGGGMPVGAL
                     GGKREIMSFLAPEGPVYQAGTLSGNPLAMAAGLATLKEIEKINFFEDLSNATNKLTEA
                     LADAAENANIPFFAASLGGMFGFCFTDKNSVENYLDVASSDEVLFKKFFHAMLAQGVY
                     FAPSMYEAGFVSSMHGDLEIQKTYDAAELVLNQLKSA"
     gene            5438..6964
                     /locus_tag="VAH76_002031"
     CDS             5438..6964
                     /locus_tag="VAH76_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386640.1"
                     /translation="MLILSKRRGLWSSMPQGTFALFFIQVVSTLSFSVLYSTLVLYMK
                     GKLGLPIATANSIMGVFIAYNFALHLLGGFWGGRLLSNRSLFCVGMIAQIVGCVLLSI
                     GNTTFLYYGLAAFLTGSGLNVTCVNCMLTQQFAPEDSRRETAFLWNYAGMNIGFFIGF
                     SLSGAFQISQNYQRLFLLSSLGNLVALLICLYFWQQLHDKNTTYSRKDKPAQRKATAL
                     GIGLIFVLPFILRQLLQYADWANKLVIVTGIVMLILILILAQQQSTKEARDKIYGFAV
                     LMLVGTVFWMLYQIGPMGLTVFIDHNVQRQYGNWIIPPQWFQNINTVVIVFGGPLLGF
                     ILNRMRQNGSQINIPTQFAFALFFIGVAFAILPIGINYANQHGMVNPGWVVISFILQS
                     IGELLISPIGYAMIGALAPTSLQGVMMGMWMLNTGVGATLSSYSSNLMIIGQESTSPL
                     LTNEGYSHVFLMLGLFAIGSSVILFILVPKLRKLIKEKNLNGFKQSGLEVTERVALCE
                     "
     gene            6957..8957
                     /gene="mnmC"
                     /locus_tag="VAH76_002032"
     CDS             6957..8957
                     /gene="mnmC"
                     /locus_tag="VAH76_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEO1386641.1"
                     /translation="MSSPFAPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFV
                     EGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKH
                     PLKKNDLIKCLQKWPELSVQAEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECY
                     EQLLFCGDINLEQQLRESYVNAWYLDGFSPSKNQSMWSDNLLTVIAMLSKEGTTVATY
                     SASSIVKTALTNAGFVIEKRKGFGPKRHMLCAYYEKAYSSSKKNRHTPWHINYPVTKD
                     ERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSP
                     FTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELG
                     QLVDEKQSSELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYN
                     QKNWVVNDIEASVLILANGQQVNYFHETNHLPVKAIRGQITTIQSTQESTKLKIPLCA
                     EGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNVLDHWAG
                     IRAASPDYLPIVGPLPNALEFKEVYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLT
                     TIPLATEWLAGLINKEISILPRKLIQAISPARFLRKKIIQGP"
     gene            9043..9828
                     /locus_tag="VAH76_002033"
     CDS             9043..9828
                     /locus_tag="VAH76_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEO1386642.1"
                     /translation="MQNIEFYQIDAFTDKVFHGNPAAVCILDDWLNDELLKAIAAQNN
                     LAETAFIIKDDQDFHIRWFTPNGEINLSGHATLAAGFALLESNKGPSDSIIFHHSSGQ
                     ISVEKRKEKFVLNFPRIGYKKTPSADPIVSLLDQPAIEVFETDLDYLVLLEHESQIRN
                     LQIDLLALAKLPKRGIIVTSTTTEADFYSRCFYPKHSIAEDPVTGSAHCILAPFWAEK
                     LNKNSLYAIQGLHRKGELYCELQSNRVLISGHCRWYLRGHLVI"
     gene            10046..11164
                     /gene="prpC"
                     /locus_tag="VAH76_002034"
     CDS             10046..11164
                     /gene="prpC"
                     /locus_tag="VAH76_002034"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEO1386643.1"
                     /translation="MSSKSGGLAGVVAGQSAIATVGLAGKGLNYRGYSINDLAEYASF
                     EEVAYLLHYGELPTQKELDVYIKKLVNLRHIPDVLKTVLKLIPKNTHPMDVLRTACSF
                     LGTIEPEDSFKQQYEIADRLLALFPGIMCYWYAYHFKNKEISGLSEENTTGGHFLALL
                     HGRKPSKIETDMMNVSLILYAEHEFNASTFAARVTAATLADFYSAITSAIGTLRGPLH
                     GGANEAAMELIERFKTPDEAAIGIKQMLASKELIMGFGHRVYTTCDPRSDIIKKWSFR
                     LGEEKGNLLLYHISERIEEIMWQEKKLFPNLDFYSASAYHYCDIPTHLFTPIFVMSRI
                     TGWSAHVFEQRANNKLIRPTSEYIGPDSRPFPAIETRG"
     gene            11166..12614
                     /locus_tag="VAH76_002035"
     CDS             11166..12614
                     /locus_tag="VAH76_002035"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEO1386644.1"
                     /translation="MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMD
                     TLGCGILALNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDF
                     NDTWLAAEWGHPSDNLGAILAVADYLCRESCHHGRPHILMHDVLTAMIKAHEIQGCLA
                     LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRAVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKHFKFQRPYGS
                     YVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIELITHESAIRIISKQG
                     ALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRD
                     YHDPDKRSIANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFS
                     TDMVEKIIHTMSDTNTLSSMKVSDFMAMWMVN"
     gene            complement(12734..13549)
                     /locus_tag="VAH76_002036"
     CDS             complement(12734..13549)
                     /locus_tag="VAH76_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO1386645.1"
                     /translation="MKRYVFMLSDGTGITAETLGNSLITQFENIQFEKITIPYIDSTH
                     RAESVVLRINQCFSEQGTKPLVFMTLVDPEIRQAIKKAHACVFDLFSIFIGPLENELE
                     EKSSYTVGRTHGVANVKSYSHRIEAIDFALSHDDGIKTRGYDKADIILIGVSRCGKTP
                     SCLYMALQYGILAANYPFTEEDLVGFRLPEVLRPYKQKLFGLTIDAQRLQQIRSERRP
                     NSKYASAEQCRLEVTEVEAMYQRENIPYINSTKYSIEEISTKVLAIAGLQRKI"
BASE COUNT         4261 a         2434 c         2617 g         4338 t
ORIGIN      
        1 atttttaaat tagagtttaa ttctcttaga ggaacaagaa caaaatctcg taaatggaaa
       61 caaggatgtg gaacgattaa gttttttgtg cgaattaatc tatagccata taaaatgata
      121 tcaatatcta atgttcttgg cccccaaaac tttctcctga ctcgcttgtg cctgttttct
      181 attttctggc accacttaag gagcttatgg ggtaacagag tagtttgtat ctctataacg
      241 acattacaaa attcctgttg attttcaaag ccccaggcct tattccaata taagcgagac
      301 gccttgataa ggcatgttga tggaatctgt ttgatttctt taatggcttg tcttatttgg
      361 cgctcaggat ttttttgatt cgatcccaag ctcagatagc aaacattcat tttgctattt
      421 tccgtttacg acgtttttta ggccgcccgg gcgttgttgc agccaattgg gttaccatgt
      481 tatttttttc aacatcatcg acctcttgaa acgtggccca ccactgcgcc agttccattg
      541 actcatcacc agataatgcc ctaagagcca taaaatcata cgctgctctg aatcgaggat
      601 gaagaagcaa attaaatgct ctgccaccaa atcttttttc gaagcgatat tgcaaaagcc
      661 aaatttccct tataacctgg ctataccttt taggtatcga aataattgca ttctgttcac
      721 taagcactat agacatggct ttttcaatcg agggtaaagg cggaagccca gattcttgca
      781 gttttctgga acactctatc attggaaacc ataacaaaac agcaataaga aatgccggag
      841 taacaggctt tccatcacgt attcttgtat cagtattttc aagtgctatg cccaaaagcg
      901 gattaaccgg gtaatgacta tcaaatagag cagctgtttg aggaaataga taattaaata
      961 acccatactc gaccaataat ttttgaaccg attcggcttc tccgcattga taaagtttcg
     1021 ttatttcatc aaatagtcgc gaactggaaa catgggttat aagatggctt attttgggga
     1081 atggagcttt ggtttccgga gataaattaa attgcagctt ggcactaaac ctgacagcac
     1141 gtaacatacg gacaggatcc tcttgataac gcgtaacggg atcgccaatt attcgaatta
     1201 acttttgttt aacgtcttca acaccaccag taaaatcaat gattgaagaa tcagaaatat
     1261 tataataaag agagtttatt gtgaaatcac gtctccatgc gtcttcgtcc agagagccat
     1321 aaacattatc tcttacaagc atgccgcgtt cattaaattg gtggcttgca tcgctagaat
     1381 tatgaccacg aaaagttgct acctcaataa tttcacgatg aaataaaata tgtaccagtt
     1441 taaatcgacg accaatgata cgagcatttt tgaaaagatt ttttacctca ttgggagtcg
     1501 catttgtcgc gatatcaaaa tctttaggcg ctttatggag cagtaaatct ctgacactgc
     1561 ctccaacgat ataggcctga aaaccatggc tgattaaacg gttaagaact ttaagcgcat
     1621 tattacttat atctgctttc gatatgttat gtttatttct cgggatgata taagccaagt
     1681 caattgtatg accatgcgac ctgtttcttc gtaacagttt tttaataaac ttgatgattg
     1741 ttgtttctct cttaattgat taaacagtaa ttcggttgtg ttttcaatta tcctgcaagt
     1801 ttaatattta ctttacagaa atattcaatt ctataaagct taacacgtaa acttttcaga
     1861 taattattta atctaaattg ctaacaccaa gcaattataa ctaagcaaat gatcaaagtg
     1921 aatcatttac ctttacaaat attttagaga aaataatata tcttgcactt tattaggata
     1981 ataaatggat tttattgata taggccttag tttaactgct ttgattattc ttgaagttgt
     2041 tcttggaata gataatttgg ttattctatc gattcttacg gaaaaattac cacgtgaaaa
     2101 aagaaaaaag gcgaggagat ggggcttaac atttgcctgg atgacacgac ttcttttact
     2161 aggctcagca gtttttttag ttaagttagt aaaaccaatt ctgacaatag gcagtctggt
     2221 attttctgct cgtgatctgt tcctcatatt aggaggtgct tttttaattt ggaagtcaac
     2281 tgatgaaatt catcaggacg ttatgaatga accattaatg gagcctgttt caaaaaatgt
     2341 ttcttcatct gccactttca ggggagttgt tattcaaatc gcattgcttg atattatatt
     2401 ttcactagat agcgttctca cagccgtagg gctaacatct cattttatgg tcatggcagt
     2461 ggcaattacc atcgcaatta tcattatgat ttgggcgagt gagcctgtaa gtaaatttat
     2521 tagcgaacat cccaccatta aaatgctggc gcttagttat ttgattttaa taggaaccgt
     2581 tttggtagct gatggttttg aattccatgt gcccagggga tacttgtatt ttgctatggg
     2641 tttttcatta gcagttgaat cactcaactt gattaaaaat gaacgtgtaa aacgtaaaaa
     2701 attaaaaaga gaataagtat gttatttatt aaagcttttc atattatcgc cttggtagcc
     2761 tggttcgctg ggttgtttta tttaccacgg ctctacgtat accacgctga atcattagat
     2821 aaaattagcg ttgatcggtt taaaataatg gaaagaagat tatattacgg aattacatgg
     2881 cctgcagctc ttgcaaccac atttcttggg ttgtggctat tcacttacaa tttatcttat
     2941 tatcttaaag cagggtggat gcatatgaag cttgcattgg ttttgctctt atgggtatat
     3001 catttaactt gcggccactt ccttaaatca ttcgcccttg gaaagaatac aagaagttct
     3061 cgtttttacc gattttttaa tgaaatgcct acattgctgc tggtaggcat tgttttgttg
     3121 gtggttgtaa aaccatttta atccatttca accatttcga aatcttcttt tccagcacca
     3181 caatcaggac aaaaccagtt ttcaggaaca tcttcccatc ttgtgcctgg ctcaatgcca
     3241 tcttctggcc aaccttcagc ctcatcataa ataaaaccac aaataacaca aatgtatctt
     3301 ttgtactctt gcatagtcta aactcaataa aaaagtgcta atttatctac tgttgatgat
     3361 tatgtaaagt taaaccttat ttaatatcat aataaatttt aaattaaacc ttataatgaa
     3421 cattataact tcgtcgagaa ttgacatggg tttgctaaaa tcaaccccaa aaaccagtca
     3481 atgtggttat cttttaaaga caggccctgc cactattttt tgcaatattc atgaaatgat
     3541 tgtaatattt tcttgcctca gggttatcag tctaatttga ttagatagtt tttaataaaa
     3601 tcattatttc gtcaatcaga aagagtacca gtagaaaaat attttaacat tttttgtata
     3661 ataaatttct gaaacatctt gaatattaaa aataataact gatatttgtc taaagtttat
     3721 catttttggg attttctatg agccgttctt cagatttatt tcataaagca caaacaatca
     3781 ttcccggagg tgttaactca ccggttcgag ccttcaaagg tgttggaggt gaacctgttt
     3841 ttttcaaatc tggcaaaggt gcctacctga cggatgtaga tgataaacaa tatattgatt
     3901 atgttggatc atggggacca cttatcctcg gacactgcca tcccaaggtg atagaagctg
     3961 tggataatgt attacatagt ggtatgagtt ttggtgctcc aacagaactg gaaatacaat
     4021 tagctgaaaa aattgcttcc ctaatgccat caatagaaaa aattcgcatg gtgaactcag
     4081 ggactgaagc aactatgact gcaattcggc tggcgcgcgg attcactaat aaaaataaat
     4141 ttattaaatt taatggctgt tatcatgggc atagcgatag cctattagtt aaagcgggat
     4201 ctggtttgct tactctcgga attccttcaa ctccaggcat tccacaaagc attaccgaac
     4261 atactttaac ggctgatttt aataacctgg aacaagttgc tcaactcttt gaaaaatatc
     4321 caaacgatat cgctactgta attttggaac ctgttcctgg caatatggga tttatcctac
     4381 ctaagataga gtttttaaaa ggattacgtg agctttgcga tcaatataat gctttgttaa
     4441 tttttgatga agtaatgact ggatttagag ttggtttaca tggtgcacag ggcttgtttg
     4501 ggattaaacc tgatatcaca acacttggaa aaatcatagg tggtggtatg cctgtaggtg
     4561 ctttaggcgg taaacgtgaa attatgtcct ttctggcgcc tgaaggccct gtatatcagg
     4621 ctggtactct ttcaggaaat ccattggcta tggccgcagg tttggctact ttaaaagaga
     4681 ttgaaaaaat taattttttt gaagatttaa gcaatgcgac taataaatta accgaggcat
     4741 tagctgatgc ggcagaaaac gcaaacattc cattttttgc tgcatcattg ggcggaatgt
     4801 tcgggttttg ttttacagat aagaatagtg ttgaaaatta tttggatgtt gcttcctcgg
     4861 atgaagtttt atttaagaaa ttttttcatg ctatgcttgc tcaaggagta tattttgcgc
     4921 catccatgta tgaagctgga tttgtctcca gtatgcatgg tgatttggaa atacaaaaaa
     4981 catatgatgc tgctgaattg gtactgaatc aattaaaatc tgcctgatag cagaattgta
     5041 ccttattacc ctaagttact taaatacaat ctttaaaatc agtatttggg taattaagtt
     5101 cagtaaagcg tctaaatgtg attgcagaac agatttttaa actggttaac tgcaatttta
     5161 aataattaat aaaacactag acagcagttt ttccctatgt catattccat tctctagatc
     5221 gcaagtatgg aatcttggtg aaaaatcaga tttgcgcaga ataagtaaaa agtttctaat
     5281 tggacctaat aacacaactg cagtttgatt attaaaatat atttaattca ttttagatac
     5341 tttttcactt gctgacgaaa ggtggttttc tctgatttgt ccacaagggt tagtgttaat
     5401 attcaagaag agctcaacta gcaagggaaa gcatataatg ttgattttga gtaaaagaag
     5461 aggactatgg agttccatgc cccaaggtac atttgcatta ttttttatac aagtcgtttc
     5521 tactttaagc tttagtgttc tttattcaac tcttgtattg tacatgaaag gtaaacttgg
     5581 tttacccata gccacagcaa acagcatcat gggggtattt atagcctaca attttgcgct
     5641 gcatctgtta ggtggttttt ggggtggcag actgctctct aaccgctcct tattttgtgt
     5701 tgggatgatc gcgcaaattg taggatgtgt tttgctctct ataggaaata caactttttt
     5761 atattatggc ttggctgcat tcttaacagg ttcagggtta aatgtaacat gtgtaaattg
     5821 tatgctgact cagcaatttg cacctgaaga ctctcgaaga gaaaccgcat ttctctggaa
     5881 ttatgcggga atgaatattg gcttttttat tggatttagc ctcagtgggg cgttccaaat
     5941 ttctcaaaac tatcaaaggc tttttctttt aagcagttta ggtaatcttg ttgctttgct
     6001 tatatgcctt tatttctggc aacaattaca tgataaaaac actacttact caagaaagga
     6061 caaacctgca caaagaaaag caacagcatt ggggattggg ctaatttttg tacttccatt
     6121 tattttaaga caattattgc agtatgctga ttgggccaat aaattggtta ttgtcactgg
     6181 aattgttatg ctcatattaa tcttgatatt agcccagcaa caatctacaa aagaagcaag
     6241 agacaagatt tatggatttg cagtattaat gttggttggt actgtatttt ggatgttata
     6301 ccaaattggc ccaatgggat taaccgtatt cattgatcat aatgtgcaac gacaatatgg
     6361 taattggatt attccgccac agtggtttca aaatatcaat actgtagtca tagtatttgg
     6421 agggccacta ttgggtttca tcttaaatag aatgcgtcaa aatggatctc agataaatat
     6481 ccctactcaa tttgctttcg cattattttt tataggggtt gcttttgcta tcttaccaat
     6541 aggtatcaat tacgctaatc agcatggtat ggttaatccg ggttgggtag taataagctt
     6601 tatattacaa agtattggcg aacttctaat atctccaatt ggctatgcga tgataggagc
     6661 gttggcccca acttcactcc agggtgtcat gatgggaatg tggatgctga atactggagt
     6721 tggtgccact ctatccagtt atagctcaaa cttaatgatt attggtcaag aaagtacttc
     6781 tcctttatta accaatgagg gttacagtca tgtctttttg atgcttggtt tgtttgcaat
     6841 aggatcatcc gttatccttt tcattttagt gcctaaacta agaaagctga tcaaagaaaa
     6901 aaacttgaat ggctttaaac agtctggctt agaggtaact gaaagagtag ctttatgtga
     6961 gtagtccctt tgccccaatt ataacggcag atattgattg gcgtgatgat ctgccgtatt
     7021 ccttacaatt tgatgatatt tactattctg ctgaaggtgg aattaatcaa agtctttatg
     7081 tttttgtcga aggtaataat ttgattaatc gttggcaaca attaccaacc aatgaatcaa
     7141 acgtatttac aatcgctgaa actggtttcg gtacaggtat gaatttctta ttaacctgga
     7201 aactatggga aaaatttgca cctcaaaatg cccgcttaca ttatatttcc tgtgataaac
     7261 acccattaaa aaaaaatgac ctaatcaaat gcttacaaaa gtggccagaa ctgagtgtac
     7321 aggctgaaaa attaatagaa cactatcctg ttttgacacc tggttatcat catttagcat
     7381 ttagtaacaa tcagattact ttaacattaa tgctaggaga cgtgctggaa tgctatgagc
     7441 aattactatt ctgtggtgac attaaccttg aacagcaatt gagagaatcc tatgttaatg
     7501 cttggtacct ggatggattt tcaccatcaa aaaaccaatc catgtggtct gacaatctgc
     7561 tcactgtcat tgccatgcta tctaaagaag gcaccaccgt agctacttat tccgcttcca
     7621 gtatcgtcaa aactgcttta accaatgcag ggtttgttat tgaaaaaaga aagggatttg
     7681 gacccaaacg acacatgtta tgcgcttatt atgaaaaagc ttattcaagt tcaaaaaaaa
     7741 atcgccatac cccatggcac ataaactatc ctgtgacaaa ggacgaaaga acagcgctca
     7801 ttgttggagg agggcttgca ggttgtttta ttgcaaatag cctcgctaag cgtggatggg
     7861 aagtgaccat attagaagaa aaagagaaag tgggatgtgg tggatcagca aatcaacagg
     7921 ctgtattgtt ccctaaatta tccacctata aatcaccatt tactcaattt atgctttatt
     7981 cgtttcttta tgctaatgac gtttacaagg aacttttaaa acattatgac cttggtgaat
     8041 taaaaggttc tttgttgtta gcgcataacg agagggaaaa agcaaaccag caaagtctta
     8101 ttcattggct agaattatac cctgaattag gacaattagt ggatgaaaag caatcttctg
     8161 aattatcagg gatatccttg ccatgtggtg gtttatttat tccatcttcc ggctggatta
     8221 attcccctga actatgcgat attctaattg ataataaaag aatttctcta atcactggca
     8281 atagagttca atctattaac tataatcaaa aaaactgggt agttaatgac atcgaagcct
     8341 cggtactaat tttggcaaat ggacagcaag taaattattt tcacgaaacc aatcatttgc
     8401 ctgtaaaagc cattagagga caaataacta cgattcaatc aacacaagaa agtaccaaat
     8461 tgaagattcc tttatgtgcc gaagggcatg ttttaccagc tttaaacaat agtcatagag
     8521 tgggggcaag ctatgatatt ggcacatctg aacctgaact taatgctctg gatgatcaat
     8581 taaacctcga ccgattaaaa cggatagcgc ctgatataat gtggtcccaa aacgtgttag
     8641 accattgggc tggtatacga gccgcatcac cagattactt gcctatagtg ggacctctcc
     8701 ccaatgcgct tgagtttaaa gaagtttatt cagaattaaa atctaactcc aaacgatgga
     8761 tagctgaagc agctccttgt tatccaaatc tatatgtgtg tgcggctttt gggtcaagag
     8821 gattaacaac aatacctctc gcaactgaat ggttagctgg tttaattaat aaggaaattt
     8881 caatactgcc cagaaaattg atacaagcta tatcgcccgc acgattctta agaaaaaaaa
     8941 ttatacaggg accataagag atatattgaa gatatattga taattcgact taacttcatc
     9001 tttctttatc agttataatt ttaattttta tgtgagctga ttgtgcagaa tattgagttt
     9061 tatcaaatag atgcttttac ggacaaagtc ttccatggta atccggctgc ggtttgtatt
     9121 ctcgatgatt ggttaaatga tgagctatta aaagccattg ctgcgcaaaa taatttggca
     9181 gaaaccgctt ttattataaa agatgatcaa gattttcata ttcgttggtt tactcctaat
     9241 ggggaaatca atctatcagg acatgcaact ttggcagcag ggtttgcttt acttgagtcg
     9301 aataaaggac ctagtgattc tattattttt caccactcca gtggacagat ttcggtagaa
     9361 aagagaaagg aaaagtttgt tctgaatttt ccaagaatag gttataagaa aactccttca
     9421 gcagacccaa ttgtatcact attggatcaa cctgctatag aagtatttga aacagacctt
     9481 gattatctgg ttttacttga acatgagtca cagattcgaa atttgcaaat cgatttgctt
     9541 gctttagcaa aactccccaa aagaggaatc attgtgacct caacaacaac agaagccgat
     9601 ttctattccc gatgttttta tccaaaacac agcattgcag aagatcctgt taccggctca
     9661 gcacattgca tattagctcc tttttgggca gaaaaactca ataaaaacag cctatatgct
     9721 atccagggat tacacagaaa aggggagtta tattgtgagc tccagagtaa tagggtattg
     9781 atttccggcc attgtcgatg gtatctacgc ggacatttag taatttaata aaatcgattg
     9841 agatgcattc atcattgaag cataatataa aacccattta ggagccaatg gagcgtgata
     9901 acaatatcca ttccaaaagg aattctctcc aagagaacaa ggtaaaataa ttaaccgctt
     9961 caaagttcag taataataaa aaaacatacg cagcaatttt gactgcacta gaaataaaaa
    10021 ctagaccaac tgatggagga agaaaatgag tagcaaaagt ggaggtttag ccggtgtagt
    10081 tgctggtcaa tcagcaattg ccacagttgg actggcaggc aagggattaa attacagagg
    10141 ctattcaata aatgatctgg ctgaatatgc aagttttgag gaggttgctt atctgcttca
    10201 ttatggcgag cttcctaccc aaaaggaact tgatgtatat ataaaaaagc tggttaacct
    10261 aagacatata cctgatgtgc ttaaaacagt tttgaaactt atacctaaaa acactcatcc
    10321 aatggatgta ctgcgaacag catgttcttt tttaggtact atcgaacctg aagatagttt
    10381 taagcaacaa tatgaaatag ctgatcgatt gctagccctt ttcccgggaa taatgtgtta
    10441 ttggtatgca tatcatttca aaaataaaga aatctctggc ttaagtgaag aaaacacaac
    10501 aggaggacac tttctggcgt tattacacgg acgcaagccc agcaaaatag aaaccgatat
    10561 gatgaatgtc tcattgatct tgtatgctga acacgaattt aacgcctcta cctttgctgc
    10621 aagagttaca gcggcaacac tggcagattt ttactcagcg atcacaagtg ctataggaac
    10681 attgcgtggt cctttgcatg gtggcgctaa tgaagcagct atggagttaa ttgagcgctt
    10741 taaaacccct gatgaagcag cgataggcat aaagcaaatg cttgccagca aagaactgat
    10801 catgggattt ggacatcggg tttatacaac ttgtgatcca cgctcagata ttattaagaa
    10861 atggtcattt agattaggtg aagaaaaagg aaacctactt ttataccata tttcagaacg
    10921 gattgaagaa atcatgtggc aagagaaaaa actatttccc aacctggatt tttatagtgc
    10981 atccgcttat cattactgcg atataccaac tcatttgttt accccaattt ttgtgatgtc
    11041 acgcatcaca ggatggtctg cgcacgtatt tgagcaaaga gcaaataata aactaataag
    11101 acctacttct gaatatatag gcccagattc cagaccattt cccgctattg aaacacgagg
    11161 ttaatatgca caattatgta gaggataatg ttaagccaga ctatgatcag gttataacag
    11221 atatagctga ttttgtatta aataaagaaa ttgatagtgt gcaagcctat gagactgcac
    11281 ggttgtgctt aatggataca ctcggttgtg gcatattggc ccttaacttt caggaatgta
    11341 ccaaattaat gggacctata gtaccgggag caactcttcc tggaggcgcg agagtaccag
    11401 gtactaatta tgaactggat ccggttcaag ctgcctttaa catcggtacg atgatacgct
    11461 ggctagactt taatgatact tggctcgctg cagaatgggg gcacccctct gataatttgg
    11521 gcgctattct ggctgttgcc gattatttgt gccgggaaag ttgtcatcat ggacgtcctc
    11581 acattttgat gcatgatgta cttacggcaa tgattaaagc acatgaaatt caaggttgtt
    11641 tggcactgga aaatagcttc aatcgcgtcg gccttgatca tgtcatatta gtcaaagtag
    11701 caagtgctgc tgttgctgct catttgtttg gagcagatcg tgatatgatg ctaagaactc
    11761 tctcgcaggt ttttgtggat ggacaaagct tgagaacata tagacatgct cccaatgcag
    11821 gttcaaggaa atcatgggca gcaggagatg ccacctcccg tgcggttcgc ttggctttaa
    11881 ttgccaaggc gggggaaatg ggatacccca gtgccttgac agcacaaaca tggggatttt
    11941 atgacgtgct atttggtaaa aaacacttca aatttcaacg cccttacggc agctatgtga
    12001 tggaaaatgt gttattcaag ttatcgtatc ctgctgaatt tcatgcacaa actgctgtcg
    12061 aatgtgcagt aaaattacat cctatagtaa aaaatagatt agaagacatt tctagaattg
    12121 aactaatcac acatgagtca gcaataagaa taataagtaa gcaaggtgct ttatataatc
    12181 ctgccgacag agatcactgc ttgcaatata tggttgcagt aggacttctc tttggtgatt
    12241 taaaggcaga gcattatgaa gacgacgttg ccgcagatcc cagaatagat gctttacgag
    12301 ccaaaatgca tgtaactgaa aatgagcagt tttcacgcga ttatcatgat ccggacaaac
    12361 gctctatagc taacagcatg cgaatcatct ttaaagatgg ctcagaaagt gaattaataa
    12421 ctatagaata ccctattggt cataaacgga gacgagaaga ggggattccc gttttaatgt
    12481 ctaaattcaa gcgtaatctg agtacaagat tctctactga catggttgaa aagattattc
    12541 ataccatgag cgacactaac acgctatcat ctatgaaagt aagtgatttt atggccatgt
    12601 ggatggtaaa ttaaaaaaac taacacccac gctcaatctg ttatctgtac tgattttagc
    12661 acagaccagg agggggctaa gcgtatagca aagccaaccc tccttactaa atcgtggcct
    12721 tcaaaaatag gttttatatt ttcctttgta aaccggcaat agcaagaacc tttgtcgata
    12781 tttcttctat ggaatatttt gttgaattga tataaggaat attttctcgt tggtacatgg
    12841 cttctacttc tgttacctct aatctgcact gctctgcgga ggcatatttg ctatttggtc
    12901 tacgttcaga gcgaatttgc tgcaaacgtt gtgcgtcaat tgttagtcca aataattttt
    12961 gtttataagg tcttaatacc tctggtaagc gaaaacccac caaatcttct tctgtaaaag
    13021 gataattggc agctaaaatg ccatattgca gtgccatata taagcaactg ggagttttgc
    13081 cacaacgtga aactcctata agaataatat ctgctttatc gtaaccacgc gtttttattc
    13141 catcgtcatg tgaaagggca aaatcgattg cttcgattcg atgggaatag gatttcacat
    13201 tcgcaacccc atgagtcctg cctactgtat aagatgattt ctcttcaagc tcattttcca
    13261 atggtccaat aaaaatacta aacaaatcaa aaacacaggc atgcgccttt ttaatggctt
    13321 gtctaatttc tggatcaact aaagtcatga atactaaggg cttggttcct tgctctgaaa
    13381 aacattgatt aattcttagt acaacacttt ctgctctgtg agttgaatct atgtagggta
    13441 ttgttatttt ttcaaattga atattctcaa attgagtaat cagactatta cctaatgtct
    13501 cagccgtaat tcctgtacca tcagaaagca taaaaacata acgtttcata agtggccttt
    13561 ttaattcatt gattaataaa taaataatat ttaaattata aatggaaata gcaattgtac
    13621 ttattaatag ccatctgcta tcttaaaaat
//
LOCUS       DATNSN010000091        13545 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.091, whole genome shotgun sequence.
ACCESSION   DATNSN010000091 DATNSN010000000
VERSION     DATNSN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13545
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.091"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            74..700
                     /gene="lvgA"
                     /locus_tag="VAH76_002037"
     CDS             74..700
                     /gene="lvgA"
                     /locus_tag="VAH76_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LvgA"
                     /protein_id="HEO1386646.1"
                     /translation="MADGDIEIKAGFVDTDLDDRKLTMIDDLNNPLAIVERVYLIWWH
                     WADFHLHVISPHIDTITPAIVIEPELISGSNEHEFVYSIHDSGSKLSTSKSQDMFSAG
                     MSMCKLFYTIEKMVHILVDRLKSGGVSMEEEVQIAFAGHEIAQRKAFESIINLPYNVV
                     VTNFDPGIWGEKYLQNVKRLADKGYGYPPESPRENYKHPVSSATTARK"
     gene            complement(730..1107)
                     /locus_tag="VAH76_002038"
     CDS             complement(730..1107)
                     /locus_tag="VAH76_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEO1386647.1"
                     /translation="MFFIKYLGALIYDLIILFILFFAYTSVLLLFTHGQAIPPATRWY
                     QFSLLSITLVYYYGSIRCGGQTVGMKAWRFKLTTDSNKTLSRQQIIARFFYFIPATLI
                     APFCLKGNYVLLHQWTQTRFKKV"
     gene            complement(1257..2396)
                     /locus_tag="VAH76_002039"
     CDS             complement(1257..2396)
                     /locus_tag="VAH76_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEO1386648.1"
                     /translation="MIAKRPIYSQQLKCVAFEILSCRNVILNEELTRQFYELIYHTDT
                     QLPLFVPFSLKKLLEQFETPINNPIILKLPADEIESVYSLTELQDSLFSIALLVNTPQ
                     QLAWLNFAEYIGLTEQLMNKSDVTKVVQYSKAKNRKIIAYDLNQHVNFEKCRAMTMDY
                     YCGDFLLNYIAEEHTEIAANKLNVLQLIQELQKEDCDLNTITHIIQEDPLLSYQLLKL
                     VNSVGFSSGPSIESIDQAVARLGTINLKKWVMLFSMKNISDKPVEILESGLIRAHMAE
                     ELAKKSSNIFSQSAYTAGLLSILDCLLNKPMPALVEQITLAEEIKNALTNKSGPLGNL
                     LSLVIAYEEGHWEEVTQLNINGIDLSQLYIDSLALVSQGTKIMSN"
     gene            2717..3091
                     /gene="sdhC"
                     /locus_tag="VAH76_002040"
     CDS             2717..3091
                     /gene="sdhC"
                     /locus_tag="VAH76_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEO1386649.1"
                     /translation="MNKKRPINLDLSSLKFPPMAIASILHRVSGVLIFLLFPFVLFIL
                     GCSLQSEESFAQTKIILANPYYKLLLWAFGASMIYHVIAGIRHLIMDMGFGESLRAGR
                     MSAVSIIALAVILAIFLGIWIW"
     gene            3085..3432
                     /gene="sdhD"
                     /locus_tag="VAH76_002041"
     CDS             3085..3432
                     /gene="sdhD"
                     /locus_tag="VAH76_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEO1386650.1"
                     /translation="MVNNVTSLTGNGLKDWLIQRVTAVYFAAYLIFLFGFILANPDLN
                     FSQWHLLFANKIFQIATTIGLMALTLHAWIGIWTVTTDYMKCTVIRLSVQLLVVLWLL
                     GQFIWGLMIIWGQ"
     gene            3434..5203
                     /gene="sdhA"
                     /locus_tag="VAH76_002042"
     CDS             3434..5203
                     /gene="sdhA"
                     /locus_tag="VAH76_002042"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEO1386651.1"
                     /translation="MTVARNRFDAVIIGAGGAGMRAALQMTNSGLKVALLSKVFPTRS
                     HTVSAQGGITAALGNADEDDWRWHMYDTVKGADYIGDQDCIEYLCKTGPEAVYELEHM
                     GLPFSRMDNGKIYQRQFGGQSKHFGGEQAARTCAAADRTGHALLHTLYQQNLKAKTHV
                     FSEWYALDLVKNSQGRIAGVTAMCMETGEIVFFQTRVCILATGGAGRIFQSTTNAFIN
                     TGDGFGMALRAGIPLQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKDGERFMERY
                     APRVKDLASRDVVARAMALEIRAGKGFDPKGVDHVKLKLDHLGADLIMSRLPGIRELS
                     MKFAGVDPIVEPIPVVPTCHYSMGGIPTNMHGQVITKTDGKEHVVEGLYAVGECACVS
                     VHGANRLGGNSLLDLVVFGRAAGIHVEELWQSNQLPDMSYVSEDDVVASLVRYERWEN
                     SKDGESHAVIRDEMQRVMQEDFGVFRTGEVMASGLKRLQALRDRLAHAKLDDKSQIFN
                     TERVEAMELDNLMATAYATALSAIARTESRGAHSREDYPKRDDANWIKHTLYFEEGEV
                     IDYRPVNTSPKYVEPFEPKERVY"
     gene            5216..5938
                     /locus_tag="VAH76_002043"
     CDS             5216..5938
                     /locus_tag="VAH76_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEO1386652.1"
                     /translation="MADSRNVILSIYRYNPEVDAKPYMKDYEIEIPAKSDPMLLTLLE
                     RLKAEQDPSITYRRSCREGVCGSDGMNINGTNGLACITHLSQLKTDKIVIRPLPGFPV
                     IRDLAVDMTQFYQQYERIEPYLQNDEVAPARERLQSPEERAQLDGLYECILCACCTSS
                     CPSYWWNPDKFVGPAGLLQARRFLADSRDKATQHRLDLLQDPFSVFRCRTIMNCTNVC
                     PKGLNPTQAIAKIRTQMLTQET"
     gene            5996..8806
                     /locus_tag="VAH76_002044"
     CDS             5996..8806
                     /locus_tag="VAH76_002044"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEO1386653.1"
                     /translation="MSSSDLQKEWASSYLSGGSMAYVDSLYEDYLADPDSVSEDWRAV
                     FSALPKVNGATKEVSHRDIRDYFLQNADKKLNKIIQIADSQQYQVASLINDFRSLGHL
                     AAKLDPLEMTERMPVPRLELAYHNLADVDVNRTFFAGTSFNGPEMTLGEIYNALRETY
                     CRSIGIEYMHISDTEVTEWLQHKMESVRGCPEFSKQEKLNILKDLIAADGLERYLGTR
                     YVGQKRFSLEGGDSLIPMMKELIRRSGVNQVKELVIGMAHRGRLNVLVNVLGKEPGLL
                     FQEFEGKIKYERTGDVKYHLGFSSDIKTESGAIVHLALAFNPSHLEIIGPVVEGSVRS
                     RLGRRNDLAKKDKVVPIVIHGDAAFAGQGVVMETFNFSQARGYCTGGTIHIVINNQIG
                     FTTSNPLDARSTLYCTDVAKMVQAPVIHVNGDDPEAVVFATQVAFDFRMKFKRDIVID
                     LVCYRRHGHNEADEPSVTQPKMYKKIKSMPTLREKYGEQLVSVNLLTKTELDKLVDSY
                     REALDKGKAVVDLVHEDYEGKQSLDWTPYINAKWTDKVDTTISKEELEKISSQLNKLP
                     DGMKLHPVVERLLAERNKMTAGEIPMNWGYAEIMAYASLLHEGYGVRISGQDSGRGTF
                     AHRHAALHDIEKGDTFIPLEHISTNPKRYFTVIDSVLSEEAVLAFEYGFASSEPSFLV
                     IWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLVMLLPHGYEGQGPEHSSARLERYM
                     QLCAQHNIQVCTPTTPAQIFHLLRRQVIRNFRKPLIVMTPKSLLRHKLAVSPLEALFK
                     GKFHTVIPEIDALDAQKVTKVVLCCGKVYYDLLQMRRDKNLNHVAIVRIEQLYPFPKK
                     ALMAELEKYQKANEVVWCQEEPQNQGVWFSSQHNIRDCLRSDQTLQYAGREFAAAPAV
                     GSPGLHAEQQVALVEQALLGKK"
     gene            8842..10071
                     /gene="odhB"
                     /locus_tag="VAH76_002045"
     CDS             8842..10071
                     /gene="odhB"
                     /locus_tag="VAH76_002045"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEO1386654.1"
                     /translation="MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKV
                     VLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKIS
                     ESESVSAKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
                     TAEVQKESSMRALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVM
                     DMRSQYKDSFEKKHGVKLGFMSFFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIA
                     VSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQGGTFTITNGGVFG
                     SLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFL
                     VSVKELLEDPSRLLLNV"
     gene            10134..11297
                     /gene="sucC"
                     /locus_tag="VAH76_002046"
     CDS             10134..11297
                     /gene="sucC"
                     /locus_tag="VAH76_002046"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEO1386655.1"
                     /translation="MNLHEYQAKQLFASYGLPVPRGEVAYNVEDALLVASQLSTSRWV
                     VKAQVHAGGRGKAGGVKLVSSKDELAAVAKSMLGTRLVTYQTDARGQPVNAILVEETC
                     EIDKELYLGAVVDRATRRVVIMASTEGGVEIEKVAHETPEKIFKVVVDPLVGVMPFQC
                     RETAFKLGLKDAQIKQFTHLMMGLGKMFVDCDLSLLEINPLVITKSGQLICLDGKINI
                     DGNALFRQPKLKNMRDVSQEDDRENRASDWELNYIPLDGTIGCMVNGAGLAMATMDVI
                     KLHGGEPANFLDVGGGATKERVSEALKIIVSDEKVKGILVNIFGGIVRCDLIADGILA
                     AVKEVDVKIPVVVRLEGNNAQLGAEILNKSNLNVIAATSLTDAAKKIVAAVSE"
     gene            11372..12247
                     /gene="sucD"
                     /locus_tag="VAH76_002047"
     CDS             11372..12247
                     /gene="sucD"
                     /locus_tag="VAH76_002047"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEO1386656.1"
                     /translation="MSVLVDKHTKVLCQGFTGKQGTYHTEQALAYGTKMVGGVTPGKG
                     GQMHLGLPVFNTMREAVEETQADASVIYVPAPFCKDSIIEAAEAGVKLIVCITEGVPV
                     LDMLQVKVYIENNTQARLIGPNCPGIITPGECKIGIMPGYIHLPGKVGIVSRSGTLTY
                     EAVKQTTDKGFGQSTCIGIGGDPIPGTSFIDALSLFEKDPKTEAIIMVGEIGGSAEEE
                     AAEFIKSHVKKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKYAALEAAGVKTVR
                     SPADLGDAIAEVTGW"
     gene            12669..13316
                     /gene="pdxH"
                     /locus_tag="VAH76_002048"
     CDS             12669..13316
                     /gene="pdxH"
                     /locus_tag="VAH76_002048"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEO1386657.1"
                     /translation="MSKFRSLADIRRDYGELQLSEESAENDPISQFKLWFDDVLLNEK
                     NDPTAMVLSTVDEKGYPDSRVVLLKGLENGNFIFYTNYQSAKAMQIQKNPYAALNFYW
                     PQMARQVRVRGRVKKISSEQSDAYFSSRPLKSQFSAIVSPQSQEILDRISLEDALNQL
                     IEEYGQKPVVRPENWGGYMIIPDEIEFWQGRDNRLHDRIHYYRHGHEWTHRRLAP"
BASE COUNT         4104 a         2251 c         3048 g         4142 t
ORIGIN      
        1 ggtaggttta atgttctatt gtcaattttg ggctaatata tcaataatat ccttactacc
       61 cgggtaaata taaatggcag acggcgatat cgaaatcaaa gcaggtttcg tagatactga
      121 tctagacgat cgcaaactga caatgataga tgatttgaat aatccattag cgatagtgga
      181 gcgagtctat ctgatatggt ggcattgggc agattttcat cttcatgtta tttctccaca
      241 tattgatact attactcctg cgatagtcat cgaacccgaa ctgatatctg gttctaatga
      301 gcatgaattt gtttattcca ttcatgatag tggctctaaa ttatctacat ccaaaagcca
      361 ggatatgttt agtgcgggca tgtctatgtg caaacttttt tatactattg aaaaaatggt
      421 tcatattctg gtagatcgct taaaatctgg cggtgtcagt atggaggaag aggttcagat
      481 agcttttgct ggccatgaaa tagcccagag aaaggctttt gagtcaatta ttaatttacc
      541 ttataatgtt gttgtgacta attttgatcc cggaatatgg ggtgaaaaat atttacaaaa
      601 tgtgaaacgc ctggcggata aagggtatgg ttatcctcct gaatccccaa gagaaaatta
      661 taagcatccg gtatcttctg caactactgc acgaaaataa ataattagcg agaatggctc
      721 aacagtgttt taaacttttt taaaacgcgt ttgggtccat tgatgcaaaa gcacataatt
      781 cccctttaag caaaaaggtg ctattaaggt ggcaggaata aaataaaaaa atcgggcgat
      841 aatttgttgt cttgatagtg ttttattaga atcagttgtg agcttaaatc tccaggcttt
      901 catacctaca gtctgtccac cacagcgaat agaaccataa taataaacaa gagtgatact
      961 gagtaatgaa aattgatacc aacgggtggc aggaggtatg gcttgtccat gggtgaataa
     1021 tagcaggaca gaggtgtatg caaaaaataa aatgaataag atgattaaat cataaatcaa
     1081 tgcacctaag tatttgatga aaaacataac taatacatat cctttattga tttcaactat
     1141 taaattttgg aatattaatc atttaggaaa acacattcta aattttttgt tgctcaaata
     1201 gaagcctggg tcaggcatag aaaaataaaa agaatattgt ttaaattgtt gcgcaatcag
     1261 ttactcatga tttttgtacc ttggcttacc aaggctaaac tgtcaatata taattgactt
     1321 aaatcaattc cattgatgtt aagttgagtt acttcttccc aatgcccttc ttcataagca
     1381 attaccaaag acaatagatt tcctaatggt cctgatttat ttgtcagagc attttttatc
     1441 tcctcagcta gggtgatttg ttctaccaat gctggcatgg gtttgtttaa taagcagtct
     1501 aatatggata ataatcctgc agtataggca ctttggctga aaatattcga acttttttta
     1561 gcgagctctt ccgccatatg agctctgatt agacctgact ccaatatttc tacaggttta
     1621 tctgagatat ttttcattga aaacagcata acccattttt tcaggttaat ggtgccaagc
     1681 ctggcaacgg cttgatcgat ggattctatg cttggtccac tagaaaatcc aactgaattg
     1741 accagtttta gaagttgata gcttagtaat ggatcttctt ggatgatatg agtgatcgtg
     1801 tttaaatcgc aatcttcttt ttgtaattcc tggatgagtt gaagtacatt taatttgttt
     1861 gctgctattt ctgtgtgctc ctctgcaatg tagttaagca ggaaatcgcc acagtaataa
     1921 tccatcgtca tagccctaca tttttcaaaa ttaacgtgtt gatttagatc ataagcaatg
     1981 attttacgat tttttgcctt actgtattgt actaccttgg ttacatccga tttattcatt
     2041 aactgctcag tcaatcctat gtattcggca aaatttagcc aagccaattg ttgaggggta
     2101 ttaaccagta aggcaataga aaatagagag tcttgtagct cagttagtga ataaaccgat
     2161 tcgatttcat ctgcgggtaa ttttaaaata atggggttgt tgattggcgt ctcaaattgc
     2221 tcaagcaatt tttttaggga aaaaggaaca aataaaggta attgggtgtc tgtgtggtaa
     2281 atgagctcat aaaattgcct tgttaattct tcattaagta ttacattgcg acaggataat
     2341 atttcaaagg cgacacattt taattgttga ctatatattg gtcttttggc gatcataaag
     2401 atctcctttt gatcatatta tgtaatcata gtacgggttt ttgtaattgg ccaatggact
     2461 attcattttc cttgtaaact gaactttgcg tgataaaaac agagtgctat acttcccatg
     2521 catgaattga aatttagtaa ttctaaatag tttttaagac ccaatttttt atttttcaaa
     2581 gccatttgtt gcgcagtata cttaatgaat tatgtttctc aataagtcta aagggtgaat
     2641 gtgaaataat tgaatgggat tcaatattta gaagaaaata tattgatgac attttcttat
     2701 gaggaagaaa ttaatagtga acaagaaaag accgatcaat cttgatttaa gcagtttgaa
     2761 atttccgcct atggctatcg catcgatatt acatagggtt tcaggagttc ttatattttt
     2821 attatttccc ttcgtattgt ttattcttgg ttgttccttg caatcagagg aatcttttgc
     2881 acagactaaa ataattttag ctaatcccta ctataaactt ctgttatggg ctttcggcgc
     2941 ctctatgatt tatcatgtca tcgcaggtat aagacatctg ataatggata tgggatttgg
     3001 tgagagtctt cgtgctggtc gtatgtcggc cgtttcaatt atagctcttg cagttatttt
     3061 ggcaattttt ctaggaatct ggatatggta aacaatgtca ccagcttaac aggaaatggg
     3121 ttaaaggatt ggttaattca aagagttaca gctgtctatt ttgcagccta tttaattttt
     3181 ttatttggat ttatattggc aaatcctgat ttaaattttt ctcaatggca tcttttgttt
     3241 gccaataaga tatttcaaat tgcaacaaca attgggttga tggctttaac attgcatgcc
     3301 tggataggta tttggacagt aactacagat tatatgaaat gtactgttat acgtttatca
     3361 gtacagttat tggttgtttt atggctcctt ggtcaattta tctggggcct tatgatcatt
     3421 tggggacaat agcatgacag tggcacgtaa cagatttgat gcggtaatta taggagctgg
     3481 cggtgcagga atgcgagctg cactccagat gacaaattct ggtttaaaag tagcgttgtt
     3541 atcaaaagta tttccaaccc gctcacatac tgtgtcggct caaggtggta ttacagccgc
     3601 tttgggaaat gctgatgaag acgactggcg ctggcacatg tatgataccg tgaaaggtgc
     3661 tgattacatt ggtgatcagg attgtataga gtatctatgt aaaaccgggc ctgaagcagt
     3721 ttatgaactg gagcatatgg ggctaccttt ttcacgaatg gataatggaa aaatctacca
     3781 acgtcagttt ggtggccaat caaaacattt cggtggggaa caagcggccc gtacctgtgc
     3841 tgcggcagat agaacagggc atgctttgtt gcacactctt tatcaacaga atttaaaagc
     3901 taaaactcat gtatttagtg aatggtatgc tctcgatctg gtaaaaaact cgcaaggaag
     3961 aattgcaggt gttactgcta tgtgtatgga gactggcgag attgtatttt ttcagactcg
     4021 agtttgtatt ttggcgactg gaggagccgg tagaattttt caatctacaa cgaatgcttt
     4081 tattaatact ggcgatggct ttggaatggc actaagagct ggtatacctc ttcaagacat
     4141 ggaaatgtgg caatttcatc caactggtat agcgggagct ggtgttctgg ttacggaggg
     4201 ttgccgaggt gaaggtggtt atttaataaa caaagatggt gagcgtttta tggagcgtta
     4261 tgctcccaga gtaaaggact tggcttcccg tgatgtggtc gctcgcgcta tggctttgga
     4321 aattcgggct gggaaaggat ttgatccaaa aggtgtcgac catgttaagt taaagcttga
     4381 ccatctgggc gctgatttga ttatgtctcg cttgcctggc attcgtgagt tatcaatgaa
     4441 gtttgccggt gtggatccaa tcgttgagcc gattccagtt gttccaactt gccactacag
     4501 tatgggagga atacccacca acatgcatgg ccaggttata actaaaactg atggcaaaga
     4561 gcatgtggtg gaggggttgt atgctgtagg ggaatgtgcc tgcgtctcag tgcatggtgc
     4621 caatcgatta ggtggtaatt ccttattgga tttggttgtg tttggccgtg cagccgggat
     4681 acatgttgaa gaattatggc aatcaaatca attgcctgat atgtcttatg taagtgaaga
     4741 cgatgtagta gcttccctgg ttcgttatga gcgttgggaa aactcaaagg atggtgaaag
     4801 ccatgcggta atacgagatg aaatgcaaag agtaatgcag gaagattttg gagtattccg
     4861 taccggtgag gtaatggctt caggactgaa acgtttacaa gccctgcgcg atcgattagc
     4921 acatgctaaa cttgatgata aaagtcaaat ttttaataca gagcgcgtag aagctatgga
     4981 attggataat ttaatggcaa ctgcttatgc aacggcttta tctgcaattg cgcgaactga
     5041 aagcagagga gcgcacagca gggaagatta tccaaaacga gatgatgcaa actggattaa
     5101 gcacacattg tattttgagg aaggtgaggt gatagattat cgtccggtta atacctcacc
     5161 taagtatgtc gagccttttg agccgaaaga acgcgtttac taatgaggat attttatggc
     5221 tgatagtaga aatgtaatct tgtcgattta tcgctataat cctgaggtgg atgccaagcc
     5281 ttacatgaaa gattatgaga tagaaatccc ggctaaaagt gatcccatgt tacttacctt
     5341 gcttgagcgc ttgaaagctg agcaagaccc ttctattact tacagacggt catgccgaga
     5401 aggagtatgt ggctctgacg gtatgaatat caatgggact aatgggttag cttgcattac
     5461 acatctttca caattaaaaa cagataaaat tgttattcgt cctttgccag gttttccagt
     5521 aattcgtgat ttggcagttg atatgacaca attttatcag cagtatgaac gcattgagcc
     5581 ttatttacag aatgatgaag tagcacctgc tcgagagagg ttacaatctc ctgaagagcg
     5641 ggcacaattg gatggcttgt atgagtgcat tttatgcgct tgctgtacca gttcatgtcc
     5701 ttcttattgg tggaatcctg ataaatttgt aggccctgct ggacttttac aagctaggcg
     5761 ttttttagca gacagtcgtg acaaggcaac acaacatcgt ttggatttat tacaagatcc
     5821 tttcagtgta tttcgttgtc gtactattat gaattgcacg aatgtatgcc cgaagggact
     5881 caatccaacg caagcgattg caaaaattcg cacgcaaatg ttgacgcagg aaacgtaata
     5941 aatcagtcct tgttactcag aaagtttctg agtgaaccct ttggagagaa tgataatgag
     6001 cagttctgat ctgcaaaaag aatgggcttc ttcctatttg tctggaggaa gtatggctta
     6061 tgttgatagt ctttatgagg attacctcgc agaccctgat tcagtgtcag aggattggag
     6121 agcggtattt agcgccttac ccaaagtaaa tggtgctacc aaagaagtat ctcatagaga
     6181 cattcgcgat tattttttgc agaatgctga caagaagctt aataaaataa ttcaaattgc
     6241 agatagtcaa cagtaccagg ttgccagttt gattaatgat tttagatcat tagggcatct
     6301 tgccgcaaaa ctggatccat tggaaatgac ggaacgcatg ccagtaccgc ggctggagct
     6361 tgcttatcat aatcttgccg atgttgatgt taatcgcact ttttttgctg gtaccagttt
     6421 taatgggccg gagatgactt tgggggaaat atacaacgcc cttcgcgaaa catactgtcg
     6481 cagtataggc attgagtata tgcatatatc cgatactgaa gtcactgaat ggttacaaca
     6541 taagatggaa tcagttcgtg gctgtcctga atttagtaag caggaaaaat tgaatatcct
     6601 taaagatttg atagcagcag acggtttgga gcgttatcta ggaacccgat atgttggtca
     6661 aaaacgattt tcgcttgagg ggggagattc tttaattcca atgatgaaag aactcattag
     6721 aagatctggt gtaaatcagg ttaaagagct tgtcattgga atggcacata gagggcgctt
     6781 aaatgtgtta gtgaatgttc tcggtaaaga accaggacta ttatttcaag aatttgaagg
     6841 aaaaattaaa tacgaacgaa ctggcgatgt gaagtatcat ctgggttttt cctctgacat
     6901 aaaaacagaa tcaggtgcta ttgtgcacct ggctttggcg tttaaccctt cacacttgga
     6961 aattattggg ccagtagttg agggatccgt acgttcacgt ttgggaagac gcaacgattt
     7021 ggctaaaaaa gataaagtag ttcccattgt cattcatggt gatgctgctt ttgcaggtca
     7081 gggcgtggtt atggaaacct ttaacttctc ccaggcacgt ggttattgta ctggtggaac
     7141 gatccatatt gttatcaata atcagatagg atttacaacg agcaatcctt tggatgcccg
     7201 ttcaactctc tattgtacag acgttgccaa aatggttcag gcgccagtta ttcatgtcaa
     7261 tggagatgat cctgaggcag tagttttcgc tactcaagtc gcttttgatt tccgcatgaa
     7321 gtttaaacga gacatagtaa ttgaccttgt ctgttacaga cgtcatggcc acaacgaagc
     7381 tgatgaacct tcagtgaccc agccaaagat gtataaaaaa atcaaatcca tgcctacatt
     7441 acgtgaaaaa tatggagaac aattggtcag tgttaattta ttaactaaaa cagaattaga
     7501 caaattggtc gattcctaca gagaagcact agataaaggc aaggcggttg tcgatttggt
     7561 tcatgaagat tatgaaggaa agcaatcttt agattggact ccttatataa atgccaagtg
     7621 gacggataaa gtagacacaa ccattagcaa ggaagagttg gaaaaaattt catcccaatt
     7681 aaacaaattg cctgatggaa tgaaactgca tcccgttgtg gagcgtttgt tagctgaacg
     7741 aaataaaatg accgctggtg aaatccctat gaattggggt tatgctgaga tcatggctta
     7801 tgccagctta ttgcatgaag gctatggcgt aagaatttct ggacaagata gtggtcgagg
     7861 aacttttgcc catagacatg ctgcactcca tgatattgaa aaaggagata cttttattcc
     7921 tttggaacat atatcaacaa atcccaagcg ctattttacc gtgatagatt cagttctttc
     7981 cgaagaagcg gtattggctt tcgaatatgg ctttgcttcg tctgagccat ctttcctggt
     8041 tatttgggaa gctcaatttg gtgattttgc aaatggagca caagtcgtta tcgatcaatt
     8101 tataagttcc ggtgagcaaa agtgggggcg gctgtgcgga ttggtgatgc tattacccca
     8161 tggctatgaa ggacaaggcc ctgagcactc ttctgcgcga ttggagcgtt atatgcagct
     8221 ttgtgcacag cacaatattc aagtatgcac tccaacaact ccagcacaaa tatttcattt
     8281 gttgagaaga caagttattc gtaatttcag gaagccttta attgtcatga ctcccaaaag
     8341 tcttttacga cataaattgg ctgtttcacc cttggaagct ttgtttaaag gaaagtttca
     8401 tacagtaatt ccggaaatcg atgccttgga cgcacaaaaa gtaacaaagg tggtgctatg
     8461 ctgtggtaag gtttattatg atttattgca aatgcgtcgc gataaaaact taaaccacgt
     8521 agcgattgtc cgtatagagc aattgtatcc atttcccaaa aaagcattaa tggctgaatt
     8581 ggagaaatac caaaaggcta atgaagttgt ctggtgtcag gaagagccac aaaaccaagg
     8641 agtctggttc tcttctcagc ataatatacg tgattgcttg cgttctgacc aaaccttgca
     8701 atatgctggc agagagtttg cagctgcacc tgcagttggt agtcccgggt tgcatgcgga
     8761 gcaacaagtt gctttggttg agcaagcatt acttggtaaa aaataaatct aaacaatata
     8821 atattaagag aaggtataac catgtctatt gaagtcaaag tacctgttct acccgagtca
     8881 gtagctgatg caacagtggc tgcgtggcat aagaaagtgg gtgataaagt atctcgcgat
     8941 gagaacctag ttgatctgga aacagataaa gttgtgcttg aagttccatc acctgtggat
     9001 ggggtattgt ctgacattct gtttaatacc ggagatacgg ttggttcagg agacttactg
     9061 gcaaaaattt ctcaaagtgt ttccgtagaa tctcaaaaaa ctgagaaaga agagaagcct
     9121 gtcaaaaaag aagaaataaa aatttccgaa agtgaatcag ttagtgcaaa agaagataaa
     9181 tcaaccagtc cggtagtaag acggatgatg gctgaaaatg atttgaaagc cgggcagatt
     9241 caaggtaccg gtaaagatgg caggataaca aaagaagatg ttttatctta catagaatcc
     9301 aatagagaaa aatcaaacca aactgctgaa gttcaaaaag aatcttctat gagagcactt
     9361 tcttttggtg gcagggaaga aaaacgtgtc cctatgacga gactaagagc caagattgcg
     9421 gaacgtttat tacaagcaca gcacaatgct gccatgttaa ccacttttaa cgaagtgaat
     9481 ttaaaggcag ttatggatat gcgttctcag tataaagaca gctttgaaaa aaaacatggt
     9541 gtaaaacttg gatttatgtc tttttttacg aaggcggtag tggagtcgct taaacgattt
     9601 ccggctgtaa atgcttcaat tgatggccaa gatattgttt atcatggctt ctatgatata
     9661 ggaattgctg tttctactga aagaggttta gtcgttcctg tgattagaga cgctgatcag
     9721 ttaagtatgg cagaaattga attagccatt aatgacgctg catcaaaagc cagaaccgga
     9781 aaattatcta tggaagagat gcaagggggg acatttacca taactaacgg tggagtcttc
     9841 ggttcactat tagctactcc cattatcaac ccaccgcaaa ctggtatttt agggatgcat
     9901 aaaattgagg aaagacctat tgtagagaaa gggcaaatag taatccgtcc tatgatgtat
     9961 gttgctttat cctatgatca tcgtttaatt gatggaaaag attctgtaca atttttagtg
    10021 agtgttaaag agctgttgga agatccatcc cgacttttac tgaatgttta atgagtcatg
    10081 tttaactgca agacccgtct tgacgggttt tgctttttta aaaggtaatt agaatgaatt
    10141 tacatgaata tcaagccaag caattgttcg ctagctatgg tttaccagtt cctcgtggtg
    10201 aggtcgctta taatgttgag gatgccttgc tcgttgcttc tcaattatca acctctcgat
    10261 gggttgtgaa agctcaagtc catgctggtg gaagaggtaa agcaggtggt gtgaaactgg
    10321 tttctagcaa agatgaattg gctgcagttg cgaaatccat gttaggcaca cgtctggtta
    10381 cttatcaaac tgatgccaga ggtcaaccag ttaatgccat tttggttgaa gaaacctgcg
    10441 aaatagacaa ggaattatat cttggtgctg ttgttgatag agccaccaga cgagttgtca
    10501 ttatggcctc cacagaaggt ggtgttgaaa ttgaaaaggt tgctcatgaa acacctgaaa
    10561 aaattttcaa ggtggttgtt gaccccttag ttggtgttat gccctttcaa tgtcgtgaaa
    10621 cagcttttaa acttggatta aaagacgctc aaatcaaaca attcactcat ttgatgatgg
    10681 gattagggaa aatgtttgtt gattgtgatt taagtttatt agagataaat ccgcttgtca
    10741 taacaaaatc agggcaactg atttgtctgg atggaaaaat taatatcgat ggaaatgctt
    10801 tattccgaca gcctaaattg aaaaatatgc gagacgtttc ccaggaagat gatcgagaaa
    10861 atcgtgccag tgattgggaa cttaattata ttcctttgga tgggacaata ggttgcatgg
    10921 taaatggtgc tggtttggct atggctacca tggacgtgat aaaattacat ggtggtgagc
    10981 cggctaattt cctggatgtt gggggaggcg ccacaaaaga aagagtcagc gaagctttga
    11041 agattattgt gtcagatgaa aaagtcaaag gaattctagt taatattttt ggtggaatcg
    11101 ttcgttgtga ccttattgct gatggaattc ttgctgctgt aaaagaagtg gatgtaaaaa
    11161 ttcctgttgt tgtcagactg gaaggtaata atgcccagtt aggcgctgag atattgaata
    11221 aaagcaattt aaatgtgatt gctgcaacga gtttgacaga cgcagccaag aaaattgtag
    11281 cagcagtttc cgaataaaga ataaattaat tttggcatta ttggtaaggt agtttgcaaa
    11341 gcggatatgc cattaaatta cgaggttaac aatgagtgta ttagttgata aacatacaaa
    11401 agtactatgt cagggtttta ctggaaaaca aggtacttat catacagagc aagcgctggc
    11461 ttatgggact aagatggtgg ggggagttac tccaggtaag ggtggtcaaa tgcatctggg
    11521 gttgcctgta tttaatacta tgcgtgaagc agttgaggaa acccaggcgg atgcaagcgt
    11581 tatctatgtt cctgcaccat tttgcaaaga ttccattatt gaagcagcag aagcaggtgt
    11641 taaactgatt gtttgtatta cagaaggtgt gcctgtattg gatatgttgc aagtaaaagt
    11701 gtatatagaa aacaatacac aggctcgatt aattggtcca aattgcccag gaataattac
    11761 accaggcgaa tgcaagatag gtattatgcc tggctatatc catttacccg gaaaagttgg
    11821 aatcgtttca cgttcaggaa ctttaaccta cgaagcagta aaacaaacta ctgataaagg
    11881 gtttggtcaa agtacttgca taggaatagg tggcgatcca attccaggaa ccagctttat
    11941 tgatgctttg tctttatttg aaaaagatcc taaaacagaa gctatcatca tggtaggaga
    12001 aattggtggt tcagcagagg aagaggcagc tgaattcatt aagtctcatg taaaaaaacc
    12061 tgtagtatca tacatcgctg gagtcacagc acctgctgga aagcgtatgg ggcatgctgg
    12121 agcaattatc tctggtggga aggggaccgc agcagaaaaa tatgctgctc tggaagcggc
    12181 tggtgtgaaa acagttcgat ctcctgctga cttgggagac gcaatagctg aggtaactgg
    12241 ttggtaagaa aatacttttt cttacttaac agctcttgca ttatagaaat aggaactgat
    12301 taaaataaat tggtggtttt cctcgaatca tgttggtgct gtgtatcaag cgcagcccaa
    12361 cagccatcat gataatattg tcacttggtt aaataagatt agatccattt cataacctgc
    12421 cctaaaacaa aatcaggtct taggaccccc aacctctttg ttaaaaattt ttttaattta
    12481 gttatttagt tttttgctat attccctcat tacaagcact tttgccttgg ccataggggt
    12541 tttcataagt cctgaaaata atcagtttat gcaagaagtc gattgaaaaa ttttaactgt
    12601 aaaatgtgtc gcaaattata aattttagag ggtataatta tttacatttt ttttagtgaa
    12661 ggtttgctat gagcaaattt agaagtcttg cggatattag acgagattat ggtgagttgc
    12721 agctcagtga agaatccgct gaaaatgatc ccatttctca attcaaacta tggtttgatg
    12781 atgtattact aaatgaaaaa aatgatccca cggcaatggt tctgtctaca gtagatgaaa
    12841 agggctatcc agattccaga gttgtcttat taaaaggatt agagaacggt aattttatct
    12901 tttataccaa ttatcaaagt gccaaggcaa tgcaaatcca aaagaatcct tatgccgcac
    12961 ttaattttta ttggcctcaa atggcaagac aagtacgtgt tcgtggtcga gtaaaaaaaa
    13021 tcagtagcga gcaatccgat gcttatttct cctccagacc tttaaaaagt cagtttagtg
    13081 cgattgtttc tccccaaagc caagaaattc tggataggat ttctttagaa gatgctttaa
    13141 atcagttgat tgaagaatat ggccaaaaac ccgtagtcag accggaaaac tggggtggat
    13201 atatgataat cccggatgag attgagtttt ggcagggtag agataacaga ttacatgatc
    13261 gaattcatta ttaccggcat ggtcatgaat ggacgcaccg tagattggca ccttgaccgg
    13321 cagtttttgt catcattcct gccggcatca ttttactatt gagatataga aggatgagtt
    13381 ttcatggcag gttagttaat ggttgaattc ttttgaataa gcacacggga ttcgcaaatt
    13441 ttcccagatt tataaatcag gattcgcgaa aaattccaaa atctttgtta aaaatatgtt
    13501 gagctttgac cttagaatga tgtgtccgtt ttatcaatat tctgt
//
LOCUS       DATNSN010000092        13244 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.092, whole genome shotgun sequence.
ACCESSION   DATNSN010000092 DATNSN010000000
VERSION     DATNSN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.092"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..467)
                     /locus_tag="VAH76_002049"
     CDS             complement(<1..467)
                     /locus_tag="VAH76_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386658.1"
                     /translation="MIHLLINWMNSKLIVRVITLNTILSLFLGAVGSLSHATKSKNIN
                     QIDSLFNIVKMSTVLSKDSLISPYNYLLTQPLMTTTLEKYYQRTAKIKVIAAFTNPHN
                     NTYSRIIILYVDRSKKRNNVDLAQKKNEIIPVELAAININFEELPEKLITDII"
     gene            complement(545..1270)
                     /locus_tag="VAH76_002050"
     CDS             complement(545..1270)
                     /locus_tag="VAH76_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEO1386659.1"
                     /translation="MENLEHGHSQKEIKERLAQIPSQNYLRDWIYGGIDGVVTTFAIV
                     SGVVGGQLSSLVILILGFANLLADGFSMAASNYLGTKSEVDQFQRYKTIEEKHIDFIP
                     EGEKNEIKQIFQNKGLHGQALDQVVEEITANRALWIKTMLQEEYGLPATLRFPLKSAL
                     YTFSAFLLFGIIPLIPYVLVMNNSFIWSCFFTAITFFVIGSIKSHWSTKSWLYSGFET
                     LIIGTVTASLSYGIGLFLHHLLT"
     regulatory      1374..1467
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1541..2485
                     /locus_tag="VAH76_002051"
     CDS             1541..2485
                     /locus_tag="VAH76_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO1386660.1"
                     /translation="MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEP
                     ADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGIN
                     SFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPMSYKTVRIGMNVTDAIYRDVIDTGIG
                     FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQ
                     RGAAYTTEKPEEAYELLCQAKPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYT
                     KHLKIIDENFDISQCYTNRFLPDTPYSDLKPIACCLEN"
     gene            2488..3558
                     /locus_tag="VAH76_002052"
     CDS             2488..3558
                     /locus_tag="VAH76_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1386661.1"
                     /translation="MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNESMSCSTAAAG
                     LLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPEAVYFQQSGSLVLCHPGDQVEWI
                     HFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
                     YLDSVCIPHITNTPVLSLKPGEINTKEQRYHYDVVFDCRGLGARNVFSDLRGIRGELI
                     WLHAPGVQLHRPIRLLHPRYSLYLVPRPGNLYLIGASEIEAEDFSPISVRTTLELLTA
                     VYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLYRHGYLIAPALAEEI
                     LRGVTSNFRHLNYPELWESYCD"
     gene            3551..3754
                     /gene="thiS"
                     /locus_tag="VAH76_002053"
     CDS             3551..3754
                     /gene="thiS"
                     /locus_tag="VAH76_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEO1386662.1"
                     /translation="MINVYFNDKIYNIQPSLSLSAFLKQLNYTQQHFAVAINNHHVPH
                     KAYETTILQANDCIDILFPMQGG"
     gene            3757..4545
                     /locus_tag="VAH76_002054"
     CDS             3757..4545
                     /locus_tag="VAH76_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEO1386663.1"
                     /translation="MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKR
                     QTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAINTAEIARELFNTHWIKLEVIGD
                     DYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
                     GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALM
                     ATAFRHAVIAGHQAFIGGMMSERNVAHPSTPLIDTPFWHQVNNL"
     gene            4542..6029
                     /gene="thiD"
                     /locus_tag="VAH76_002055"
     CDS             4542..6029
                     /gene="thiD"
                     /locus_tag="VAH76_002055"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO1386664.1"
                     /translation="MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQ
                     NAHSITAVEAISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVV
                     LDPIITSSSGTNLFFPDLQQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIIN
                     AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSS
                     ALTACLALGYSIKDAIVIAKMYVNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPT
                     PFIKPVPSFKKCSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVH
                     LANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEV
                     ARAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSD
                     VLKTKIDGVSVISAITKASVPLVAAKQFLTQMNESHNEQIKFGNE"
     gene            6001..7128
                     /locus_tag="VAH76_002056"
     CDS             6001..7128
                     /locus_tag="VAH76_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEO1386665.1"
                     /translation="MNKSNLAMNELTRYSQQIKMEEIGLDGQEKLKNSRVLCIGLGGL
                     GSPLLFYLAAAGVGVLGIVDDDIIELSNLHRQILYNHTHINKKKAVTAKKQLLAINPL
                     IQVESYSSRLTEENAAELITQYDIIADGTDNFYTRYLIHSICFELEKPYVYASASGFQ
                     GYCSIFHGKQDPCLHCIFPVSVSDINMPNCEGAGVIGTLPGMLGIIQATEIIKWILQI
                     GNPLKKRLLAINLLTMTFKNIIISRNPDCEFCIQNQSIRQLINPVNCSTQIEPCHHRI
                     TLDEFYQLFTNNKNIHLIDVRSTKEHQKQNIGGIVLPVDELPQRLHELNPNRLIILYC
                     QSGKRSKMALDILLKAGFASVKYIENGLKGVTVNYNKDMPQ"
     gene            complement(7157..7990)
                     /locus_tag="VAH76_002057"
     CDS             complement(7157..7990)
                     /locus_tag="VAH76_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEO1386666.1"
                     /translation="MIRSILVLFFLFIANAGYAWNAIGHQLVAQIAYDNLTPQSRRMC
                     DLYSHSKSKTSSNVNFVKSASWLDSIRAHDVHWFDALHYIDIPFSMDETELPALPDIN
                     ALWGINQAIAVLSSNKASIADKKLSLRILVHLVGDIHQPLHTVTKISKKLPKGDLGGN
                     LFQLAKNPIGNNLHQYWDNGGGILIGQDKFFQIKNKARQLEKKWSCQSASKEKNPQQW
                     INASHQLALTKVYKVSAHQVPGKQYQLNTQNITEKQILLAGCRLAYLLNNIAEGKNKL
                     I"
     gene            complement(7993..9252)
                     /gene="tolB"
                     /locus_tag="VAH76_002058"
     CDS             complement(7993..9252)
                     /gene="tolB"
                     /locus_tag="VAH76_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEO1386667.1"
                     /translation="MFNRIISLFLLLFTGQVIALDLELTQGINSALPIAINSFGSDAA
                     AQEIGNVIENDLTISGQFKIISGPQGANSQSSVSTLRQLGADSVVTGRVNQVGNRIEV
                     SFTLADAVANGNILLTKTFQINANQVRALAHHISDEVYQKLTGERGIFSTRIAYISVQ
                     RNGGRSRYSLEVADADGHNPQSLLVSSEPIMSPSWSPNGKSISYVSFEKKKAEIFTVS
                     VETGQRRLITSFPGINGAPAWSPDGQHLAVVLSKSGTPKIYDVDLSSGSMKQLTFGNS
                     IDTEPRYSPDGRSLLFTSGRGGSPQVYRLSLADGQISRVTFEGNYNARASYTPDMKHI
                     VMLHREDRQFNIGVQNTGGGPISNLTFSGLDESPSVSPNSRLVLYATRYQDRGVLGIV
                     SIDGRIRMRLPAREGDVQEPAWSPYLS"
     gene            complement(9258..10256)
                     /gene="tolA"
                     /locus_tag="VAH76_002059"
     CDS             complement(9258..10256)
                     /gene="tolA"
                     /locus_tag="VAH76_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEO1386668.1"
                     /translation="MISDLTYRKAFLFAVTLHLFLLVLLFTDNSSKKPVLTMESKNSP
                     GMMQPTASQQEVVKAVSVDNKEIMKTVNRLKQEREQQKKAELNRQKELLRQAEAAKQQ
                     RIKEQQEIARLKEEANKIAIARKKQVEEEKKRLKELAEQKALEAKRIEELKKQNEKLA
                     KQRELEAKKLAELNKKKQEEKEKAEKLKAEQAKAEKLKAEKARAEQEKAEMERKRQAE
                     AAAAQQAAQNAERQARIAGEVDKYKALIVNAIGRNWILPENVDSSLSSQFRIRLAPDG
                     TVLEVSLIRSSGDPLLDRSAQTAIYKASPLPVPKDVETFNLFRDISLTVRPEQVRG"
     gene            complement(10253..10708)
                     /gene="tolR"
                     /locus_tag="VAH76_002060"
     CDS             complement(10253..10708)
                     /gene="tolR"
                     /locus_tag="VAH76_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEO1386669.1"
                     /translation="MNRSKTNRERPISEINVVPYIDVMLVLLVIFMITAPMLTQGVTV
                     DLPKAASQTLQAADREPIIVSVNQLGSYFLNISSTPAEPIEAQALLVRVAAELELAKQ
                     SGQKLNVLVKGDQGVAYGKVVQAMALLKQAGAEQVGLLTDSQTGDEGQA"
     gene            complement(10717..11394)
                     /gene="tolQ"
                     /locus_tag="VAH76_002061"
     CDS             complement(10717..11394)
                     /gene="tolQ"
                     /locus_tag="VAH76_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEO1386670.1"
                     /translation="MVANQANVLMYFMQAGLVVKSVMLILLIASIISWTLIFQRAWFF
                     NRRKQMTETFTRRFWDCGDLSKLYADVDSNSDERQGVAAIFHAGFKEFVRCRKQGKVS
                     IEPIQRVMQISHAKEAEKLEQHLPFFASVGSISPYVGLFGTVWGIMTSFQALGHAQQA
                     TIAMVAPGISEALVATALGLFTAIPAVIAYNRYTTRANTLLNKYDLFQEELISLIEQQ
                     TSGSPRG"
     gene            complement(11419..11814)
                     /gene="ybgC"
                     /locus_tag="VAH76_002062"
     CDS             complement(11419..11814)
                     /gene="ybgC"
                     /locus_tag="VAH76_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEO1386671.1"
                     /translation="MNLISDHQHNIRVYAEDVDYMGIVYHANYLCYLERARTELLRQN
                     NLILSEFEKSDILFAIHELTINYVFPARLDDQLTIKTEIKEFRACSFLFNQEISNQHK
                     QLICRASVQVVCVGSNLKPKRLPNIMRNE"
     gene            complement(11818..12828)
                     /gene="ruvB"
                     /locus_tag="VAH76_002063"
     CDS             complement(11818..12828)
                     /gene="ruvB"
                     /locus_tag="VAH76_002063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO1386672.1"
                     /translation="MLESDRLISSQSIVSEDAMDRAIRPLSLSEYVGQDSVSSQMQIF
                     INAARKRNDPLDHVLIFGPPGLGKTTLANIIAHEMGVNIRQTSGPVIERAGDIAAILT
                     NLQQNDVLFIDEIHRLSPVIEEILYPAMEDYKLDIMIGEGPAARSIKLELPPFTLIGA
                     TTRAGLLTSPLRDRFGIVQRLEYYSVDSLTKIVARSAHLLGVPTKPEGAREIALRSRG
                     TPRIANRLLRRVRDYSEVKGNGIITVDMAQQALEMLEVDQHGFDLMDRKLLLAVIEHF
                     NGGPVGIDSIAAAIGEEKGTIEDVLEPFLIQQGFLMRTPRGRIATSKAYQHFGFSAIE
                     QE"
     gene            complement(12975..13100)
                     /locus_tag="VAH76_002064"
                     /pseudo
     CDS             complement(12975..13100)
                     /locus_tag="VAH76_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT         3899 a         2655 c         2494 g         4196 t
ORIGIN      
        1 tgaattatat cagtaataag tttctccggc agttcttcaa aattaatgtt aatagctgct
       61 aattcaactg gaatgatttc atttttcttt tgggcgagat ctacattatt tcttttctta
      121 cttcggtcta catacaggat gataattcga gaataggtat tattatgcgg atttgtaaag
      181 gcagcaatta ctttaatctt ggcggtgcgc tgatagtatt tttccaaagt ggtcgtcatt
      241 aatggctgtg taagtaagta attgtaagga gagattagac tgtctttgct taatacagtg
      301 ctcattttaa caatattgaa cagactatct atctggttta tatttttgga tttagttgca
      361 tgagataaac tccccaccgc tcctaaaaaa agagataaaa tggtattgag agtaattact
      421 cgcacgatga gttttgaatt catccaattg attaataaat gaatcatctc caatattgcc
      481 ccatgtaatt aactacaaaa tttatatcta acaagtactg ctaatagcag agccatgttt
      541 tattttaagt cagaagatga tgtaaaaata gtccaatacc ataagagaga gaggctgtaa
      601 ccgtgccaat tattagtgtc tcaaaaccgg aatataacca ggatttggtt gaccagtgac
      661 ttttgattga accgatgaca aaaaatgtaa ttgcagtgaa aaaacaagac cagatgaacg
      721 aattgttcat gaccaacacg taagggatta atggaataat tccaaaaagt agaaacgcac
      781 taaaagtata aagcgcagat ttaagcggga atcgaagtgt agcaggcaac ccatattctt
      841 cttgtaacat ggtttttatc cataaagctc tattcgctgt aatttcctca acaacttgat
      901 ctaatgcttg gccatgcaaa cctttatttt gaaaaatttg ttttatttcg tttttttcac
      961 cctctggaat aaaatcaata tgtttttctt caattgtttt atagcgttga aattgatcga
     1021 cctcagattt ggtacccaga taattactcg ctgccattga aaaaccatca gcaagcaagt
     1081 tggcaaaacc taaaattaga ataactaaag aagataattg tccacccaca acaccggaga
     1141 caatggcaaa agtagtgaca acaccatcaa ttcctccata tatccaatct cgcaagtaat
     1201 tttgagatgg gatctgtgct aatcgctctt tgatttcttt ttgcgaatgt ccatgctcta
     1261 aattttccat tgcagatcct ttctccatat gtgttattta ttaattatca attctgccaa
     1321 tattatcgct agtttgacaa agcatataat aaaatttagt atatttgagt ttgttagatg
     1381 gcgcggggtg tcgggaaatc cggctgagag ttacccgttg aacttgatct gaatcatatc
     1441 agcgtaagga cgccttcaaa aaaaccatgg cctttttaag gtatggttcc cttttgccat
     1501 ctccatacta tttaaaataa taacataagg agttatggcg atgtcatcac taaaatccag
     1561 ggttacccta ctgcttaatt ggtacactaa cccttatcat acccccatac ttgtggcaca
     1621 acaattgggg ttttattcag aagaagatat caagcttgcc attctggaac cagcggatcc
     1681 cagtgatgta accgaaatcg ttggcctggg aacagtagat tttggtgtta aagcaatgat
     1741 tcataccgtt gcagccaaag caaaaggtta tccagttact tccataggaa cgcttcttga
     1801 tgagccacct accggactta ttgctttaaa atcaagtggc atcaattctt tccaggatat
     1861 tgttgggaaa cgagtaggtt atatcggcga attcggcaaa aaaattattg atgatttagc
     1921 cagccttgcg ggtattgatc cgatgagcta caaaacagtc cgtattggca tgaatgtcac
     1981 agatgccatt tatcgggatg ttatcgacac tggtattggt tttattaatt ttcaaaaagt
     2041 tgaactggaa catctttgcg gagaaactgt atttttacga atcgatcaat tagctggttt
     2101 aggctgttgt tgtttctgct caatacaatt tattgttcct gaaacaacat tacagcaacc
     2161 cgaattagtc aaaggatttc tcagggcaac acagcgcggc gcagcctaca cgacagaaaa
     2221 acctgaagaa gcttatgagt tattatgtca ggctaaacca cagttgcgta ctcctttata
     2281 tcaaaaaatt tttacaagaa cattaccatt tttttcacgt acattaatta atgttgacag
     2341 agactgggat aaggttggac gctataccaa gcatttaaaa attattgatg aaaattttga
     2401 tatctcgcaa tgctatacga atcgtttttt gccagataca ccttattctg atttgaaacc
     2461 aatcgcctgt tgtcttgaaa attaattatg cgagcaggca ttgtaggtgc agggattatg
     2521 gggcgtctgc tggcttttgc attacataat gcgggctggc aggtatccct ttttgatcgc
     2581 tctaatgaaa gcatgagttg cagtactgct gctgcaggtc tattaacacc gttttctgaa
     2641 ttggataaat caattctact gatttccacc ttgggtcaag aatctattaa gaattgttgg
     2701 ccacgtatta ttgaggattt gcctgaagct gtttattttc aacaatcggg cagtctggtg
     2761 ctatgccatc caggcgatca ggtggaatgg attcatttta gtcggcgtat cctgagtcaa
     2821 ctcaatccga gtaattctgt ctttcaaaaa ttatcccatg aaaatctcat tgcgcttgag
     2881 cctgagctga caaaatttga aactgcttat tattttccaa atgaagcgca tattgattgc
     2941 caaacgctaa tgttgtcttt aaaaaattat ctcgactctg tttgcatacc tcacattacc
     3001 aatactcctg tcttatcgct taaacctgga gagatcaaca caaaagaaca aagatatcat
     3061 tatgatgtcg ttttcgattg tcgtggttta ggtgcgcgaa atgtattttc tgatttacga
     3121 ggtattagag gtgaactcat ttggcttcat gcgccaggag ttcaactgca tcgccctatt
     3181 cgtcttctgc atcccaggta cagtctatat cttgtaccca gacctggaaa tctttacctc
     3241 ataggtgcta gcgaaattga ggcagaagat tttagtccga tttcagtacg tacgactttg
     3301 gagttattga ccgctgtgta ttatttacat gggaactttg atgaagcacg catcataaaa
     3361 acggtgaccc attgccgtcc aactttagca aatcatttac cacgtattaa atttgccgac
     3421 gggctaatag cagttaacgg tctttatcgt catggttatt taatagcccc agccttggca
     3481 gaagaaattt tacgaggcgt gacatccaat ttccgtcatc tgaattatcc agaactttgg
     3541 gagagttatt gtgattaatg tctatttcaa cgataaaatt tacaacatcc aaccatcatt
     3601 atcattaagc gcatttttaa agcagcttaa ttatactcaa cagcattttg ctgtcgctat
     3661 aaataatcat catgtaccac ataaagctta tgaaactaca attttgcaag ccaatgactg
     3721 cattgatatc ctctttccca tgcaaggagg ttaatgatgt ggaatttggc aggaaaagaa
     3781 ttaagtagcc gtttgctctt aggcactgcg tgttatcctt cattagagca tatgcagcag
     3841 gcaattcata attcgggtac agaagttatt acgatatcaa taaaacgcca gacttctgca
     3901 ggattggatg gagagtcctt ttggcaagcg gtaaaaaaac tagattgtca tcttttgccc
     3961 aatacagctg gttgccgtaa tgcggaggca gcgatcaata cagctgaaat tgcgagggag
     4021 ttatttaata ctcactggat aaaactggaa gtgataggcg atgattataa tttacagccc
     4081 gaaccatttg agttaatcaa ggcagccaga attttgattg acagaggatt tgaagttttt
     4141 ccttattgca cagatgatct cgttttatgt caaaaactgg ttgatgctgg ttgtaaaatt
     4201 ttaatgccct ggggagctcc tataggctca ggtaagggtt taattaaccc ctatgccttg
     4261 gaaactctgc gttatcgatt tcctgacatc acattaatca ttgatgccgg gattggtaag
     4321 ccatctcatg ctgtacaggt gatggaattg ggttttgatg gcgtgcttct aaatactgct
     4381 gtagctcttg ccaatcatcc tgctctcatg gcaaccgcat tccgccatgc cgttattgca
     4441 ggtcatcagg catttatagg aggaatgatg tctgagagaa atgtagctca cccaagtact
     4501 ccattaatcg atactccatt ttggcatcag gtaaacaatt tatgaaaaaa ccgattgttt
     4561 ggacaattgc tggtgttgac tcatctggat tagctggtgt ccatgctgac atggaaactt
     4621 ttagtcgctt gaatgtcagg gcttgttcag tcatcaccgc agtaacagca caaaatgctc
     4681 actcaattac agcagtagaa gcgatatcca gagatcaggt tgctgctcag tgccgtgcct
     4741 tggaattaaa tttaaaaccg gatgccatta aaattggtat gttatgctct acaccaatct
     4801 gtgaagaaat agcttatttt ttaaaaggtt atgaaggatt tgttgttctt gatccgataa
     4861 ttacctcctc ctcgggaact aacctgtttt ttccagattt acaacagcat aaagaaaatc
     4921 taattcaatt gtttccgtac gttactatta ttactcctaa tcggattgaa gcagaaatta
     4981 ttttgaatcg gagcatttct tcttatcagg atataatcaa tgctgcttca gatcttttat
     5041 cactgggagc aaagcaggtg ttactgaaag gaggacatgt aaaagataac tcattcagcc
     5101 aagattactg gactgatgga aaagagttat tttggattgc aaatcgtcgt tttcctgaaa
     5161 caaattaccg tgggacaggc tgtgttctat cttcagcact cacagcttgt cttgcgctag
     5221 gctattcaat aaaagatgct attgttattg ctaaaatgta tgtcaatcgc ggcatacgcc
     5281 aatcaataga acttgataaa gacgcctcgc aactttatca cgatggttgg ccagaagatg
     5341 aggccgatct cccctattta tcaccaacac cgttcattaa accagttccc tcatttaaaa
     5401 aatgttctat gggtttttat cccattgtgg atagtagcca ctggttagaa atgcttttgc
     5461 cattgggaat taagtgcatt cagttacgaa taaaggaagc ctctcaagag cggttggaag
     5521 aagagataaa acggagtgtc catttggcaa atcagtataa tgctgcatta tttattaatg
     5581 atcactggga acttgctatt cattatggtg ctgcaggagt tcaccttggc caagaggatc
     5641 tggaaaaagc tgatgtcgat agaattaatc gagcaggttt attcttaggc ataagtacgc
     5701 attgctatta cgaagttgct cgggctcatg ctcttaaccc ttcctacgtc gcttgcggcc
     5761 ccatatatga aacaaccagc aaaataatgc catttcaagc acaaggaatt gcacgacttg
     5821 aacgttggcg aaaaacgctt cgttatccat tggttgccat tggaggtata actttaaaga
     5881 acctttcaga tgtcttaaaa acaaaaattg atggtgtgtc agtgatctct gccattacaa
     5941 aagcatcagt acctttagtt gcagccaaac aatttttaac tcaaatgaat gaatcacata
     6001 atgaacaaat caaatttggc aatgaatgaa ttaacccgct attctcaaca aataaagatg
     6061 gaagaaattg gattagacgg acaggagaaa cttaagaatt ctcgagtatt atgcattggg
     6121 cttggcggtt tgggttcgcc tctcttattt taccttgctg cagcaggtgt tggagtatta
     6181 gggattgtcg atgatgacat cattgaacta agtaatttgc ataggcagat tctttataat
     6241 catacccata ttaataaaaa aaaggctgtt actgcaaaaa agcaacttct ggctattaat
     6301 cccctgattc aagttgaatc ttactccagt agattaacag aggaaaatgc ggctgagcta
     6361 attacgcagt atgacatcat cgcagatgga accgataatt tttatacgcg atatttgatt
     6421 cacagcattt gctttgagct ggaaaaacct tatgtgtatg ctagtgcctc aggtttccaa
     6481 gggtattgct ctatattcca cggtaaacaa gatccctgcc tccattgtat cttcccggtt
     6541 tctgtaagcg atatcaatat gcccaattgc gagggggccg gggtaattgg tactttaccc
     6601 gggatgctag gtattattca ggcaacagaa ataatcaaat ggatactaca aattggcaat
     6661 ccattaaaaa agcgtttgct tgctattaac ttgttaacaa tgacctttaa gaacatcatt
     6721 atttcaagaa atccggattg tgaattctgc attcagaatc agtcaattag acaattaatt
     6781 aatcctgtta attgttcaac tcaaattgag ccatgccatc atagaataac tcttgatgag
     6841 ttttatcaat tatttacgaa taataaaaac attcatctaa ttgatgttcg tagtacaaag
     6901 gagcatcaaa aacaaaatat aggaggtatt gtgcttcctg tagatgagtt gcctcaacgc
     6961 ttacatgaac taaaccctaa tcggcttatt attctgtact gccagtcggg aaaacgaagc
     7021 aagatggcat tagatatact tctcaaggct ggctttgctt ctgttaaata tatagaaaat
     7081 ggtttaaagg gagtcactgt caattacaat aaagacatgc cccaataatt ctcccctcct
     7141 aaaaaggaag ctgaaattaa ataagtttgt ttttaccttc ggcaatatta tttagcagat
     7201 aagcgagtcg acacccagcc agaagtatct gtttttctgt aatattttga gtatttaact
     7261 gatattgctt gccgggcact tgatgagctg aaaccttata aactttagtc aatgccaatt
     7321 gatgtgatgc attaatccat tgttgcggat ttttttcctt actggctgat tggcaagacc
     7381 atttcttttc caattgtctc gccttgtttt taatctgaaa aaacttgtct tgaccaatca
     7441 gaataccgcc accattatcc caatattgat gcaaattatt cccaatagga tttttagcta
     7501 actgaaataa attaccaccc aaatcacctt taggtaattt tttacttatc tttgtaacgg
     7561 tatgcaaagg ctgatgaata tctccaacca aatgaaccaa tatgcgcaag ctcaattttt
     7621 tatcagcaat actggcttta ttggaagaca gcacagcaat tgcctgatta attccccaga
     7681 gtgcattgat gtctggcaag gctggtaatt ctgtttcatc catagaaaaa ggaatatcaa
     7741 tgtaatgtaa agcatcaaac caatgaacat catgtgctct tatagaatcc agccatgaag
     7801 ctgattttac aaaattgaca ttagacgacg tcttcgattt tgaatgggaa tataaatcac
     7861 acattcgcct ggattgaggt gtcaaattat catatgcgat ctgggctacc agttggtgcc
     7921 ctatcgcgtt ccatgcataa ccagcattag caataaataa aaaaaacaat accaatatgc
     7981 ttcttatcat tcttaactca ggtaagggga ccaagcgggt tcttgaacat ctccttcacg
     8041 agcaggaagt ctcattctaa ttctcccgtc aattgacaca atgcctaaaa cccctctgtc
     8101 ctgataacgg gtagcataaa gcactaaacg actattcgga gagactgatg gtgattcatc
     8161 aaggccagaa aaggttaaat tggaaatcgg gccaccacct gtattttgta caccaatatt
     8221 aaattgtcta tcttccctgt gtagcatgac gatatgcttc atatctggag tgtaggaggc
     8281 acgggcatta taattgcctt cgaaagtgac tcgacttatt tggccatcgg ccaatgataa
     8341 gcgatatact tgtggtgacc cgcccctccc tgaagtgaac agcaaactgc gaccatcagg
     8401 agaataacgt ggttccgtat cgatagaatt gccaaaagta agctgtttca tagaaccgct
     8461 gctcaaatca acatcgtaaa ttttgggtgt accgcttttg gataatacaa ctgccaaatg
     8521 ctgaccgtca ggtgaccatg cgggcgctcc attaattcct gggaagctcg taattaggcg
     8581 acgctgtcct gtttcaacag agacggtaaa aatttcagct tttttctttt caaaagaaac
     8641 ataggatatt gactttccat tgggtgacca tgaaggtgac ataataggct ctgaagagac
     8701 gagtaagctt tgaggattat gtccgtcagc atcagcaacc tccagggagt agcgagacct
     8761 tcctccgttt ctttgtaccg aaatataagc tatcctcgtg gagaaaatcc cccgttctcc
     8821 agtcagtttt tggtatacct catcactaat atgatgagcc aaagctctta cctgattggc
     8881 attgatctga aaagtttttg tcaacaaaat attgccattg gctacggcat cggccaaagt
     8941 aaagctcact tcaatccggt taccaacctg attaactcgg ccagtgacta cactgtcagc
     9001 gccaagttga cgtaatgtac tcacggaaga ttgagaattc gcgccttgtg gtccagagat
     9061 aattttaaat tgtcctgata tagtcaaatc attctctatg acatttccta tttcctgagc
     9121 cgccgcgtct gagccaaaag aattgatggc tataggcagg gctgaattaa ttccttgagt
     9181 tagttccaaa tcaagagcaa tcacttgacc ggtaaataac agcaagaaaa gtgaaataat
     9241 tcgattgaac acgagcttta ccccctaact tgttctggac gaacggttaa actgatatcg
     9301 cgaaacaaat taaatgtttc aacgtcttta ggcacgggaa gtggtgaagc cttgtatatt
     9361 gctgtttgtg ctgacctatc taacaaagga tcaccactgc tgcggattag agacacttcc
     9421 agtacagttc catcaggagc aagacgtatc ctgaattggc tcgacagaga actgtcgaca
     9481 ttttcaggta aaatccagtt gcgtccgatc gcattgacaa taagtgcctt gtatttatca
     9541 acttcaccag ctattcgtgc ttgtctttca gcattttggg ctgcctgttg ggctgcagcc
     9601 gcttctgctt gtctttttct ttccatttct gctttttctt gctcagcacg ggctttttcc
     9661 gctttcagct tttcagcctt cgcctgctca gctttaagtt tctcggcttt ctctttttct
     9721 tcttgttttt tcttattgag ttcagcaagt tttttagctt ccaattcccg ttgtttggcc
     9781 aacttttcgt tttgtttttt taactcttca atacgtttcg cttctaatgc tttttgctca
     9841 gccaattctt ttaaccgttt tttttcttct tcaacttgtt tttttctggc aattgcaatt
     9901 ttattagctt cttctttcaa tcttgcaatt tcttgttgtt ctttgattct ttgctgtttt
     9961 gcagcctcag cttgccttaa taattccttt tgtcgattaa gttcagcttt cttctgttgt
    10021 tcacgttctt gtttcaaacg attaacagtt ttcattattt ctttattgtc gacactgacc
    10081 gcttttacta cttcctgctg agaggcagta ggttgcatca tgccaggaga atttttggat
    10141 tccatggtta gcacaggttt tttgctgcta ttatctgtaa aaagcaatac aagtaaaaaa
    10201 agatgcagtg ttacagcaaa aagaaaagct tttctatagg taagatcact tatcatgctt
    10261 gcccctcatc accagtctga gagtcagtaa gcaaccccac ttgctcagct cctgcttgct
    10321 ttaagagtgc catggcttga acaactttcc cataagcaac cccctgatct cccttaacaa
    10381 gcacattgag tttttgacct gactgtttgg ccaactccag ttctgccgca actcgtacta
    10441 ataatgcctg ggcttcaata ggttctgctg gcgtactact aatatttaaa aaatagctgc
    10501 ctaattgatt cactgacaca atgataggtt ctctgtcagc agcctgtaaa gtttgactgg
    10561 ccgctttggg cagatctact gtaacacctt gtgtcagcat tggagcagta atcataaaaa
    10621 taaccagtaa aaccaacata acatcaatgt aaggcactac attaatttct gagattggac
    10681 gttcacgatt ggttttagat cgattcattt taagtcttat cctctaggtg aaccagaagt
    10741 ttgttgttca attaaagaaa tcagttcttc ttgaaataaa tcatatttat tcagcaaagt
    10801 attggcacgt gtcgtatatc ggttataggc aataaccgca ggaattgcag taaatagtcc
    10861 taaagcggta gcaaccagcg cttctgaaat acctggtgcc accattgcga tagtggcttg
    10921 ttgggcatgt cccaaagcct gaaatgatgt cattataccc caaactgtgc caaatagacc
    10981 cacgtaggga gaaatagatc ctacagatgc aaaaaatggt aaatgttgct ccagtttttc
    11041 cgcttctttg gcatgactta tctgcattac tctttgtata ggctcaatag atactttgcc
    11101 ttgttttctg cagcgaacaa attctttaaa accagcgtga aagatagccg ccaccccttg
    11161 tctttcatca ctattactgt ctacatcagc atagagtttg ctcaaatctc cacaatccca
    11221 gaaccttctg gtaaaggttt cagtcatttg ctttctacga ttaaagaacc atgctctttg
    11281 aaagattaat gtccatgaaa ttatcgaagc aattaataat atcaacataa cggactttac
    11341 taccaaaccc gcttgcataa aatacattag tacatttgct tgattagcca ccattaatct
    11401 ccacactgaa aggtttcgtt attcatttct cataatatta ggcagtcgct taggctttaa
    11461 attactgcca acgcaaacta cttgaacact ggccctgcaa attaattgtt tatgttgatt
    11521 agatatctct tgattaaata gaaaactaca cgctctaaat tctttaattt ctgttttaat
    11581 tgttaattga tcatctaacc tggccggaaa tacataatta attgtgagtt cgtgaatcgc
    11641 aaataaaata tcggactttt cgaattcact tagaattaaa ttgttttgcc ttaataattc
    11701 agttcgagct ctttccaaat aacaaaggta gttagcatga taaacaattc ccatataatc
    11761 aacatcttct gcatagactc taatgttatg ctgatgatct gatataagat tcatgtgtta
    11821 ttcctgttcg atcgcactaa agccaaaatg ctgataagct ttcgaagtgg ctattctacc
    11881 gcgaggagtg cgcattagaa agccttgttg tattaaaaat ggctctaaaa catcttctat
    11941 agtccctttt tcttcgccaa tagcagctgc aatactatct atgcctactg gaccaccatt
    12001 gaagtgttct atcaccgcta acaataattt tctatccatt aaatcaaacc cgtgctgatc
    12061 cacttcaagc atttccaatg cttgttgggc catatcaaca gtaatgatgc catttccttt
    12121 cacttcagaa taatccctga ctcttcttaa caggcgattg gctattcgtg gtgttcctct
    12181 ggagcgtaat gcaatttctc ttgctccttc gggttttgta gggacaccaa gcaaatgcgc
    12241 agagcgtgcc acaatcttgg taagcgaatc aacagaataa tattccaaac gttgcactat
    12301 accaaatcta tcccttaatg gcgaggtaag caaccctgct ctcgtcgttg ctccaattaa
    12361 tgtaaaggga ggtaactcca atttaataga tcgtgctgct ggcccctctc ctatcatgat
    12421 atccaactta taatcttcca tggctggata aagaatttct tctataacag gacttaatct
    12481 atgaatttca tcaataaata gtacatcatt ttgttgtaga ttcgtcaata tagcagcaat
    12541 atcgccagca cgctcaatca ctggcccaga agtttgtctg atattcactc ccatttcgtg
    12601 agctatgata tttgccagag tagttttacc aaggccaggt gggccaaaaa taagaacatg
    12661 atccaaagga tcgtttctct ttcttgccgc attgataaat atctgcatct gtgagcttac
    12721 agaatcttgc cctacgtact cgctcaagct aagcggcctg atggctctat ccattgcgtc
    12781 ttccgagaca atactttgac tacttattag gcgatcactt tcgagcataa gaggttttat
    12841 cgggttcatt cgaatgaaag cattttaaca taggtttatc taatagcaga tctttgtaag
    12901 cttatttatt taataattaa atgcaattga tttcacgatt aaggtggatg attttatatg
    12961 atatcaaaat gatattataa agtgtgattc tcacttacaa tatccatcag ctgttttcga
    13021 gctctaaata tacgtgagcg tacagtcccg atggggcaat gcatttcttt tgcaatatcc
    13081 tgataagttt gtccatcaaa tatgtgcagc ccataacata atcgtaactc ttcagataaa
    13141 catgacattg ctgcttctat ttgtttctca aactctaatc ccattatgtc agactctgga
    13201 gaattatgtg gtatagaaaa ttgttcatcc atatactgca tttc
//
LOCUS       DATNSN010000093        13212 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.093, whole genome shotgun sequence.
ACCESSION   DATNSN010000093 DATNSN010000000
VERSION     DATNSN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.093"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..1035
                     /locus_tag="VAH76_002065"
     CDS             64..1035
                     /locus_tag="VAH76_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386673.1"
                     /translation="MLKKTSLALLGLAASGYALAGTMGPVCTPGNVTVPCEAKRWDLG
                     VQALYLKPVYDADMAYRQVHVAPTRYDDDQGWDWGYRLEGSYHFNTGNDVTLTWIHYS
                     DFANPSGLVGRIDIPPLPPALAFPRLFSAIYKNRFEQVNLVMGQHADFGLVKDMRFYG
                     GLQYANIEIDNTNYYFGAFPQLGGVNLFDNTDYKGVGPVLGIDYAYALTNSLSVTANG
                     AASVLYGKGKYNIGYVALPAGSSGAVVRSIYANKRTIVPSLEAKLGLNYAYNMAQGVL
                     NLEGGYQVMNYFNALHTQFLQNMANPVTNSDYGLYGPYIGAKYIGNA"
     gene            1336..2928
                     /locus_tag="VAH76_002066"
     CDS             1336..2928
                     /locus_tag="VAH76_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HEO1386674.1"
                     /translation="MKFKKIILALACLSSPLYADQDQQLKSEIQRLQHQAEDLQAQLN
                     RLQKQLANHKSSQQKHKQQAAAKPAEPQSKPTVKSGSAIEEKYHSSKVEVHAPDAHPE
                     SISFYPTALIADNRVVTYIAGTPVVSSPFLGDRPAFDGSDYIVNISSINRDVRLLQQR
                     RRLYRAYQKIGYPIPNMPIISLSGKTEPAATFNNPFRSTNTDGDITLGSSELDVAAAL
                     NENVEAYIAIAYDESPPAIGPRVNNSAFNLNMGFVNIGNLDKSPLYFTAGQVYVPFGR
                     YSSAMVSAPVTMNLARTKTRPVIFGYKSQGDTGPFGAVYGYRSDTTLGRSGVGGVNLG
                     YIFGFDNDINGEIGGGFISSVADAGGMQSTGSNVGTTFGGFGSITNGNENVRKTKAAD
                     VHGHVGYDRYNLTLEWVGAVQSFRPQDLSFNGQGARPQAAQAELGMTFMAFNRPASIG
                     VGYQWTKEALALNLPKQRYIGVFNISIWKDTVESIEYRHDIDYGLTQFANGAAPPGFV
                     NLPTLGTGKSADTVSAQIGVYF"
     gene            3145..4650
                     /locus_tag="VAH76_002067"
     CDS             3145..4650
                     /locus_tag="VAH76_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEO1386675.1"
                     /translation="MHRDCVRQEGLYKLNNLFCKWAAVIRGLQGIIFRSRLSVSVLED
                     SDSVATKQSQLSVTCEMKLSKFMQTSCIFIFLTLFLLVGLQTSFAAKIVELNIKGPIG
                     PATADYLERGIKSAQDADLIVILIDTPGGLYDSTRNIIQLFLLSDVPIVTYVSPTGAR
                     AASAGTYLMYASTLAAMAPGTQMGAASPVSLGTGFSEGEKDEKKKSTMENKVTHDAVA
                     TIRSLAQLRGRDPDFAEKAVTEGKSITANEALSKGVVNYIAKNRDDLLSQIHGIKVSQ
                     NNKTITINTESPDIQVINPDWRTRFLSVITNPTVAYLLLLLGIYGIFFELVNPGYVLP
                     GVVGAVSMLFALYALQLLPINYAGLGLIILGILFVIAEAFTPSFGALGVGGTVSFILG
                     SIMLMNTEHLAFQIAWSAIWAMAVLNILIFVLVLGMLIKSRNQKIRHGLETLVGAKGR
                     ALGDINLEGQAVIKGEIWNVHSSSPIAANKSVKVTRASGLLLEVEEDQSVY"
     gene            4694..5449
                     /locus_tag="VAH76_002068"
     CDS             4694..5449
                     /locus_tag="VAH76_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEO1386676.1"
                     /translation="MGPFLVILLVAIGLLLVSMFKVFREYERGVVFMLGRFWRVKGPG
                     LIIIIPVIQQVVRVDLRTIVMDVPSQDVISRDNVSVRVNAVVYFRVVVPENAIIQVEN
                     YFEATSQLAQTTLRSVLGQHDLDDMLAEREQLNSDVQKILDAQTESWGIKVSNVEIKK
                     VDLDESMIRAIAKQAEAERDRRAKVIHAEGELQASEKLLQASQVLAQQPQAMQLRYLQ
                     TLATIAVNNNSTIIFPMPMEIGDILTSMVGKKK"
     gene            5688..6254
                     /locus_tag="VAH76_002069"
     CDS             5688..6254
                     /locus_tag="VAH76_002069"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEO1386677.1"
                     /translation="MSIKSDRWIEKMALEHGMISPFQAGQVRENQNGRIISYGVSSYG
                     YDVRCSNEFKIFTNINSAIVDPKAFDENSFVDVQSDVCIIPPNSFALARTVEYFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYAYEGVAQMLFL
                     EANEVCAVSYRDRNGKYQGQTGVTLPRT"
     gene            complement(6270..6581)
                     /locus_tag="VAH76_002070"
     CDS             complement(6270..6581)
                     /locus_tag="VAH76_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEO1386678.1"
                     /translation="MIKSELIEHIAARMTHLTEKQVADGINRILELMSEALIHGQRIE
                     IRGFGSFSLHYRPPRNAHNPKTGEKVVTEAKYSPHFKPGKELRERVNSSRAKFPLLDK
                     D"
     gene            complement(6834..7550)
                     /locus_tag="VAH76_002071"
     CDS             complement(6834..7550)
                     /locus_tag="VAH76_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEO1386679.1"
                     /translation="MLDFISSKMLFLCLILFLILFWLCFGKARKELRINRWQKKLNLK
                     HHAQIFHLLYGDVNGFMISQQARKNRDAIEYVYGEIEFLPFTALLSMVNIDHNTVFYD
                     LGSGTGKAVVACAMVYPVHKSIGIELFPNLHQCACERLQKLAAMEGYTESSKKISFIL
                     GDFLTVDLSEATLIFINSSTLFGATWEALNTRLNSLPQLSTVITTSKTLSSSRFKLVT
                     RAKIQMSWGVVFAFIHKKEN"
     gene            complement(7555..8280)
                     /locus_tag="VAH76_002072"
     CDS             complement(7555..8280)
                     /locus_tag="VAH76_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386680.1"
                     /translation="MEHYQEDDLARIKELLRHTGEFIAYFEFVETKMMEWREEIEQQA
                     ERVLHIAQSIQNELTSMQNLISQSGVTHFKISAEKALAQGETHLHSLERTCNQFTHIF
                     QQQQERLNALTENCIDRIEKHTGQATQTLTLQLSKYDVQHFHRIANESCDHVDRVAND
                     AINKSNKMLNMLQFKVGLFAMLITVLTAFVIVIYLNDEMPWEMHHQAMSERQAGKVLL
                     QAWPKLSQKEKAKILNEDGLKPG"
     gene            8861..9904
                     /locus_tag="VAH76_002073"
     CDS             8861..9904
                     /locus_tag="VAH76_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386681.1"
                     /translation="MPRARFFKAETNSSQVQQSFINVGGEQFHAAAVALIDYFKRSPR
                     INDDTLKKILERFYFHFPKYIPSQPYLTPSERMSMLINGVRKSEIVECFSYVLRQLAI
                     DEIYAKPLNYRDIFAEFERDTPKSLLRQPSTPLPASALRALSHVISTNITLSVVENGK
                     ELRKREVYVGDNLNSTKPGITIQVQQNNKYFPAVKNKADFVYVGQLAVSPPKPVESSN
                     TSGKDTIADMVDLIASDNKKLLQTYKQWHQNFLTMLKLGEITTQRMINLYIKFLPSEK
                     GLFVDSTEFFSRLSEDSDKPVIPDYSGENKDGVNQLLASALAGWISTRQVDVDQLFDH
                     LENRPSSVSMSVG"
     gene            complement(9999..10949)
                     /locus_tag="VAH76_002074"
     CDS             complement(9999..10949)
                     /locus_tag="VAH76_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEO1386682.1"
                     /translation="MIYPNILATIGHTPVVKINRLGKDLECELYAKCEFFNPGGSVKD
                     RIGYEMVVKAEKEGRIKPGDTLIEPTSGNTGIGIALAGAVLGYKVIITMPEKMSQEKQ
                     SVLERLGAIIYRTPTEAAYNDPDSHISLAKKLQAEIPNSHILDQYANPNNPNAHYFGT
                     AQEIIDDFGKDLHMVVAGVGTGGTITGIAKRLKEFNPAIKIIAADPEGSILGGGTEVK
                     SYHVEGIGYDFFPDVLDNTLIDAYIKTNDADSFSTARRLIKEEGLLIGGSSGAAMWAA
                     LQAAKSLRKGQKCLVILPDSIRNYMSKFANDEWMKEMGFL"
     gene            11526..11792
                     /locus_tag="VAH76_002075"
     CDS             11526..11792
                     /locus_tag="VAH76_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386683.1"
                     /translation="MINYKTIKWRYGLIALFALGVSGCYFDGASPQERGYSYQENSMP
                     SSGGQTTASNQSQPQQKSYASKDTSQKSTPGPKRTAAPQLPVIQ"
     gene            12034..12174
                     /locus_tag="VAH76_002076"
     CDS             12034..12174
                     /locus_tag="VAH76_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide"
                     /protein_id="HEO1386684.1"
                     /translation="MDKLDWELPEVCAIPIDSVTADQDGNLKSDGPDSSGLSSSSSSS
                     GG"
     gene            12265..>13212
                     /locus_tag="VAH76_002077"
     CDS             12265..>13212
                     /locus_tag="VAH76_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO1386685.1"
                     /translation="MECGNLIDILNTEKTALTSFSTHEILKTAQRMRLTNSVYTILRG
                     HYGIPVSQLACFKPVLEQNRDRAIRKLILSIKINELFQQYNIPVIHLKGLPLSQKLFG
                     DPLIRQSCDIDLFISPQHLERADEVFFENGIIRDKYSFLHDVNKLKKHRVLKDVSYVA
                     EKYGIRIELHWRLFRWKNKFNQPFEFYWNNKVPILVGGKNLFYTLSPIDEYLYVAVHG
                     ANHGFESLHWLYDFARYSTLLSMDDRRRVVELVDSLSIQTEYQMATYCAERIFPNVFT
                     ESGNAGLLPNSLTRKALISLIYHSKSSYRSKRRMLRMVAN"
BASE COUNT         3909 a         2447 c         2784 g         4072 t
ORIGIN      
        1 taattaagtt ctttttgagc ccatcaccaa aatgatgggc tttttttttg aaatggagtc
       61 catatgttga aaaagacaag tttagcccta ttgggattgg ctgcaagtgg ttatgcattg
      121 gcaggaacta tgggacctgt ttgtacgcct ggtaatgtca ctgttccctg tgaagcaaaa
      181 cgttgggatt taggtgtgca ggcactctat ctaaaaccag tttatgatgc cgatatggct
      241 tatagacaag ttcacgtagc ccctacaaga tatgatgatg atcagggttg ggattgggga
      301 taccgcttag agggttctta tcactttaat accggcaatg atgtgactct tacatggatt
      361 cactacagtg attttgcaaa tcctagtggt cttgtcgggc gaattgatat ccctccattg
      421 ccgcctgcat tagcgttccc acggttattt agcgcaatat acaaaaacag atttgagcaa
      481 gtgaacctgg tgatggggca acatgctgat tttggcttgg tgaaggacat gcgattctat
      541 ggcggtttac agtacgccaa tattgaaatt gataatacga actattattt tggtgcattt
      601 cctcagttag gtggagtcaa cctgttcgac aacactgatt acaaaggagt aggacccgtt
      661 ttaggaatcg attatgccta tgccttaact aattcgttga gtgtcacagc aaatggcgct
      721 gcatcagtct tatatggcaa gggtaagtat aacatcgggt atgttgcatt gcctgcaggc
      781 tcttctggcg cagtagtgcg atccatctat gcaaacaaaa gaacaatagt tcccagcctg
      841 gaagctaagc ttggattaaa ttatgcttac aatatggccc aaggagtatt aaatcttgag
      901 ggtggttatc aggtaatgaa ctattttaat gccctgcata cacagttcct gcaaaacatg
      961 gcaaaccctg taaccaatag tgattatgga ctctatgggc catatattgg tgcaaaatat
     1021 attggtaatg cctaaacgaa gtcctttcct taaatgcgtt cgtcatcgac gaacgcattg
     1081 cttttaaaat cctgcttaaa aatcttgaaa aaaaaattct ttagcaaaaa atataatcgc
     1141 ggtaatccat tctaagtcta atcaactaaa ccccctaatt tatggatgta taggcaataa
     1201 actataaatg catacaaata aatcaattta ttttaaattt tagcggagtg atcctatagc
     1261 cagccagtaa ttgccgcgtt ataatcccgt ggctatccgt ttaggatgta aaaataataa
     1321 acaggagtac cccgcatgaa gtttaaaaaa attattttgg ctctcgcttg tttatcatcg
     1381 ccattatatg cggatcaaga tcaacaactg aaaagtgaaa ttcaacgatt acagcatcaa
     1441 gcagaagatt tacaagccca actcaaccga ttacaaaaac aattagcaaa tcataaaagc
     1501 tctcagcaaa agcacaaaca acaggctgcc gcaaagcctg cagaaccaca gagtaagccg
     1561 acggttaaat cgggatctgc tatagaagaa aagtatcact catccaaagt cgaagtccat
     1621 gcgcctgatg cccatccaga gtcaataagc ttttatccaa ctgcattgat agccgataac
     1681 cgagtggtta cttatatagc aggaactcct gtagtaagct caccattcct cggagaccgg
     1741 cctgcttttg atggttctga ttatatagta aacatttcca gtattaatag ggatgttcgt
     1801 ttattgcagc agcgacgacg cttatacaga gcctaccaaa aaataggcta ccctatccct
     1861 aatatgccaa ttatttcatt aagtggtaaa acagaacctg ctgcgacatt taacaatcca
     1921 tttcgatcaa ctaatacgga cggtgacatt acgctaggct caagtgaact ggacgttgct
     1981 gctgcattga atgaaaatgt cgaagcttat attgcaattg cctatgatga aagccctcct
     2041 gcaattggcc cgcgggttaa taactcggca tttaatttga atatgggatt tgtcaacatt
     2101 ggtaacttgg ataaaagccc gctttatttt actgcagggc aagtctatgt tccattcggc
     2161 cgttattcca gcgcaatggt tagtgcgcct gtcacaatga atttggcaag aaccaagaca
     2221 aggcctgtca ttttcggtta taagtctcag ggtgataccg gccctttcgg tgctgtttat
     2281 ggctacagaa gcgatactac attaggtcgc tcaggagtcg gtggcgttaa tctgggatat
     2341 attttcgggt ttgataatga catcaatggc gagatagggg gcggctttat tagctctgtt
     2401 gctgatgcag gcggtatgca aagtactgga tcgaatgtgg ggacaacatt tggcggtttt
     2461 ggctccataa ctaacggtaa tgaaaacgtt cgtaaaacaa aagctgctga cgttcatggc
     2521 cacgtgggtt atgatcgtta taatctgact ttggaatggg ttggtgccgt acagagtttt
     2581 agaccccagg acttgagttt taatggccaa ggcgccagac ctcaggctgc gcaagcagaa
     2641 ctgggtatga cttttatggc tttcaataga ccggcttcca taggagttgg ttatcagtgg
     2701 acaaaagaag cattagcact aaatctgccg aaacaacgtt atattggagt atttaacatt
     2761 tccatttgga aggatacggt ggagagtatt gagtacaggc acgatattga ttatggactt
     2821 actcagtttg ctaatggcgc agctcctcca ggattcgtca accttccaac tttaggtaca
     2881 ggaaaatctg ccgatactgt ttctgcacaa attggtgttt atttctaaat cattaccatt
     2941 ctgagtagta agcttgtaac aaatcaatct gttacaagct gatgtgttta caggtctttc
     3001 tgcaacggtt gcagggtggg tatcgagcct aactcaactc acctcaataa cagataaaga
     3061 tcttgaattg gtgtgacact cccgcttgtt ttttcaacct gtgctatctt ttattttata
     3121 actacctctt aaagggatgt aaaaatgcat cgggattgcg tcagacaaga gggactgtac
     3181 aaactcaata atcttttttg caaatgggct gcggtgataa gaggtctgca aggaataata
     3241 ttcagaagca gactatctgt gtcagttctt gaggattcag attctgtagc aacaaaacaa
     3301 tcccagctat cggtaacgtg cgaaatgaaa ctcagtaaat tcatgcaaac aagttgtatt
     3361 tttatattcc ttaccctgtt ccttttggtt ggattgcaaa cttcatttgc agcaaaaatt
     3421 gtagagttga atataaaagg accaatcggc cctgcgacag cagattattt ggaaagaggt
     3481 attaaaagcg ctcaagacgc tgatttgata gtgattctaa tcgatacacc aggcggttta
     3541 tatgattcaa cacgaaatat cattcagcta ttcttacttt ctgatgtgcc catagtgact
     3601 tatgtaagtc ctactggagc aagggctgca agtgctggaa cctatttaat gtatgcgagt
     3661 acattagcgg caatggcgcc tggtacccaa atgggggctg caagtcctgt cagtcttggc
     3721 acaggattca gtgaaggtga aaaagatgag aaaaaaaaat caaccatgga aaataaggta
     3781 actcatgatg ctgtagcaac aataaggtca cttgctcaac tgagagggcg cgatcctgat
     3841 tttgctgaaa aggcagtgac agaaggcaaa tccataacag cgaatgaagc cctcagcaag
     3901 ggagtggtca attatattgc aaaaaataga gatgatttac tgtcccaaat ccatggaata
     3961 aaagtcagtc aaaataacaa gacaataact attaataccg aaagccctga cattcaagta
     4021 atcaatccag attggagaac ccgttttcta tcagtcatta ccaaccctac agtggcttac
     4081 ttattattgc tacttggaat ttatggaatt ttctttgaat tggttaatcc aggttatgtt
     4141 ttacctggag tagttggcgc agtttcaatg ctttttgcgc tgtatgcgct gcagctgtta
     4201 cctataaatt atgcaggttt gggcttgatt attttaggta ttttatttgt catagccgag
     4261 gcatttaccc caagttttgg agcgttaggt gtaggaggaa ctgtttcttt tattttaggt
     4321 tccattatgc tgatgaacac ggagcacctg gctttccaga tagcgtggtc agcaatttgg
     4381 gctatggcag ttcttaatat attgattttt gttttggttc tggggatgtt aattaaatct
     4441 cgaaaccaaa aaatcagaca tggcctggaa acgttggtgg gtgcaaaagg cagggcgtta
     4501 ggagacatta atctggaagg ccaggctgta attaagggag agatttggaa tgtccattca
     4561 agctcgccaa tcgcggcaaa taaaagtgtt aaagtcacgc gagcatcagg attattgttg
     4621 gaagtggagg aagatcagtc ggtttattag ttggttattc catgttgaat gaaaaaggat
     4681 taaggagaat gagatgggac catttctggt tatattactg gtagcgatag gtttgctact
     4741 ggtttcaatg ttcaaggtat ttcgggaata tgaacgtgga gtggttttta tgctgggtcg
     4801 gttttggcgt gttaaaggtc cgggattgat aatcattatc ccagttattc aacaagtggt
     4861 gcgtgttgat ttgcgaacca tagtcatgga tgtacccagt caagatgtta tctcgagaga
     4921 taacgtttca gtgcgagtca atgctgtggt gtattttcgt gttgtcgttc ctgaaaacgc
     4981 aatcatccag gttgaaaatt attttgaggc aaccagccaa ttggcgcaaa ccacattgcg
     5041 gtcagtattg ggtcagcatg atttggatga tatgttggct gaacgcgagc aactgaatag
     5101 tgatgtgcaa aaaattcttg atgcccagac agagagctgg ggaatcaagg tttcaaatgt
     5161 tgaaatcaag aaggtggatt tggatgaaag tatgataagg gcaatagcca aacaggctga
     5221 agcggaaaga gacaggagag cgaaagtaat ccatgcggag ggagagttgc aagcttcgga
     5281 aaaattattg caagcctctc aagtgctcgc tcagcaaccc caggcaatgc agctaagata
     5341 tttgcaaact ttagccacta tagcggttaa taacaattca acgataattt tccctatgcc
     5401 aatggagata ggtgatatat taacttccat ggttggtaag aaaaaataga ccttgaacag
     5461 tggcataagc cccaaatata cctaagtgta tagagtgctg tcattggata tgagaacata
     5521 gcgataagat tttttattcc gcgtcagctt gtggtctcag tattttttta aaatgagaac
     5581 tttcatcgaa gtttcaatca ttttattctg tataatttcc caaaacaaac tttcgtttca
     5641 ttgacttttg tgatagaaat ctatcttttt aagaaaagag gaatatcgtg tcaatcaaat
     5701 cagacagatg gatagagaaa atggcccttg agcatgggat gatttctcca tttcaggccg
     5761 gtcaagtgcg agaaaaccaa aatggtagga ttatttctta tggcgtctcc agttatgggt
     5821 atgatgttcg ttgttcgaat gaattcaaga tttttacgaa tattaattca gcgattgttg
     5881 atcccaaggc gtttgatgaa aatagctttg tagatgttca gtctgatgtc tgcattattc
     5941 cccccaactc gtttgccttg gcaagaacgg tcgaatattt tcgtataccc cgcaatatat
     6001 taacgatatg cttgggtaaa tccacttacg ctcgttgcgg gattatagtg aatgttactc
     6061 ctctggaacc ggaatgggag gggcatgtta ctctcgagtt ttccaatacg accactttgc
     6121 cagccaagat ttatgcgtat gagggagttg ctcaaatgct gtttttagag gctaatgagg
     6181 tttgtgctgt ttcttatcgt gatcgaaacg gaaagtacca gggacaaacc ggggttacac
     6241 taccacgcac ttaggatgaa caataaagct taatctttat ccaaaagtgg gaacttggct
     6301 cgcgacgaat tgacccgctc tcgtaattcc tttccaggtt taaagtgagg actgtatttt
     6361 gcctctgtta ctactttttc acccgtttta ggattatgag catttcgcgg tggtcggtaa
     6421 tgaagagaaa aactgccaaa gccacgaatt tctattcttt ggccatggat aagtgcttca
     6481 ctcattaatt caagtattct gttgatgcca tcggctactt gcttttcagt aagatgcgtc
     6541 attcgagcag cgatgtgttc aatgagttcg gatttaatca tatactcacc tcgttttgtc
     6601 gcgtatttaa tagaccgatt gcatcctcta ttattttagc ttgtctgcct atgcttctat
     6661 tcgctttgta tttcgaaaca cgggcggccg atactaaaat aaactccacg agctgagcaa
     6721 ctggtctaat gtctgctacc aagacacatc aggtgctgac aatttcccat ttgattttat
     6781 aagtatagtt cgggtaaaaa attattcaat gttttcaagc tggttagtaa aaattagttt
     6841 tccttcttat gaatgaatgc gaaaaccacc ccccaactca tttgtatttt agcgcgagta
     6901 actaatttaa aacgactgga tgataaggtt ttgctagtag taatgacggt tgatagttgt
     6961 ggcaggctat tgaggcgagt gttaagggct tcccaggttg caccgaataa ggtacttgaa
     7021 ttaataaata ttaaggtcgc ctcagataaa tcaacggtta gaaaatcacc caatataaat
     7081 gagatttttt tgctactctc tgtatagcct tccatagccg ccaatttctg gagacgctcg
     7141 caagcacatt gatgtaagtt agggaacaat tcgataccta tacttttatg gactggatat
     7201 accatagcgc aggcgacaac agccttacca gttccagagc caagatcata aaaaacagta
     7261 ttgtgatcaa tattgaccat tgataataaa gcggtgaacg gtaaaaactc aatttcccca
     7321 tacacatatt ctattgcatc tcgattcttt cttgcttgtt gggaaatcat gaagccatta
     7381 acatcaccgt acaggagatg aaaaatttga gcatgatgct ttaaatttag ttttttttgc
     7441 catcggttaa tgcgcaactc ttttcttgcc ttgccaaaac aaagccaaaa aagaatgaga
     7501 aacagaatga ggcataaaaa aagcattttt gatgatatga aatcaagcac agtattatcc
     7561 tggtttcaga ccatcttcat tcaagatttt tgccttttcc ttttgactta attttggcca
     7621 tgcttgaaga agaactttac cagcctggcg ttcgctcatg gcttggtgat gcatttccca
     7681 aggcatttca tcattaaggt aaatgactat cacgaaagca gtaagtacgg tgattaacat
     7741 ggcaaaaaga cctactttga attgaagcat attaagcatt ttattgcttt tgtttatggc
     7801 atcgtttgca actcggtcga catggtcaca gctttcgtta gcgatacgat gaaagtgttg
     7861 aacatcgtat ttagatagtt gcaaagtaag tgtttgggtt gcttgaccag tatgcttttc
     7921 gattctatca atgcagtttt ctgttagggc attgagcctt tcttgttgtt gttggaagat
     7981 atgggtaaat tgattgcaag ttctttctaa agaatgcaaa tgcgtttcgc cttgagccag
     8041 agccttttca gcacttattt taaaatgggt tactcctgat tgagaaataa ggttttgcat
     8101 tgaagttaat tcattttgta ttgattgagc gatatgtaga actcgttcag cctgttgctc
     8161 aatttcctct cgccattcca tcattttcgt ttcgacaaac tcaaaatagg caataaattc
     8221 tccagtatgt ctcagcaact ctttaatgcg agccaagtca tcttcctgat aatgctccat
     8281 gcctctctcc ttactaaatt catagtcaga gaatcttcaa atagatctct cgcataatct
     8341 attcttttca ttttattgcc acgttgcaaa cgtttctatg tttgcacttt gcctaaaaca
     8401 aaataatcag tttatacaag agagctaata atatcctttg atgcatcctg cctctttggc
     8461 cgtgaattta gaattatgat gttccatatt atattatatc cataacttta ttttggaaac
     8521 ggcaattttt gcctaaaatc ctcattcaat tttcgttatt agttccataa attctcgatt
     8581 ggtacagtat agtcatgcct cataaagttg gcattattta tgcttgaatg taatatactc
     8641 ggcatatact ttctttatta ttaaaatcca ctgagacgag ttgtcagttt gcagaaataa
     8701 tcgaagctca atggcattac atgaaaatga gcggtggttt aaaaatgatg tggttaagag
     8761 cccccgtgtt aaaagtttca gtagactgta aaaactttgt taatatttcc ttaatctgga
     8821 ttgattagaa tgaagatata agttgggata ggagaacatt atgccaagag ctaggttctt
     8881 taaagcagaa actaatagtt cgcaagtaca gcaaagcttt attaatgtgg gcggagagca
     8941 gtttcatgca gctgcagttg ctttaattga ttattttaag agatccccac gcattaatga
     9001 tgatactttg aaaaaaatac tggagcgttt ctactttcat tttccaaaat acattcccag
     9061 ccaaccttat ttgactccct ccgaacgcat gagtatgttg attaatgggg tacggaagtc
     9121 ggaaattgtc gaatgttttt cttatgtatt acgccaattg gctatagatg agatttatgc
     9181 aaaacctctc aattatcgtg atatctttgc tgaattcgag cgcgacacgc caaaatcatt
     9241 gttacgtcaa ccatccactc ctctcccggc cagtgctttg agagcgcttt ctcatgtaat
     9301 cagtacaaac atcactttat ctgttgtgga aaatggcaag gaattgagga agcgtgaagt
     9361 gtatgtgggg gataatctga atagtacaaa acccgggata accattcagg tccagcaaaa
     9421 taacaagtat ttccctgccg tgaaaaataa agctgatttt gtttatgtag ggcaattggc
     9481 agtaagcccc cccaaaccag tagaaagctc caatacaagt gggaaagaca ctatcgctga
     9541 tatggttgat ttaattgcgt ctgataataa aaaattatta cagacttata aacaatggca
     9601 tcagaacttt ctaaccatgc ttaaattagg tgaaataaca acccagagga tgatcaatct
     9661 ttacatcaaa tttttacctt cagaaaaagg cttgtttgtt gactcaacag aatttttttc
     9721 caggttatca gaagattctg acaagccggt tattcctgat tattcgggtg aaaacaagga
     9781 tggagtcaat cagttattgg cttccgcgct ggctgggtgg attagtacca gacaggtcga
     9841 tgttgatcaa ttgtttgatc atttagaaaa tcgaccttct tcagtatcca tgtcagttgg
     9901 ttaaagtgtg ttggcaattc gaatcatttt ttcaaagcga attgtcaaca gtcatttttt
     9961 gatgaaatag caataggata gtttttcatg gctttcaatc aaagaaagcc catttctttc
    10021 atccattcat cattggcaaa tttggacata tagttacgga tagagtcggg taaaatcacc
    10081 aaacattttt gtcctttgcg taaggatttg gctgcttgca atgccgccca catggctgct
    10141 ccacttgagc cgcctattaa tagcccttct tctttaatga gccgtctggc tgtactgaat
    10201 gaatccgcgt cgttagtttt tatgtaggca tcaatcaatg tgttatccag cacatcaggg
    10261 aagaaatcat aacctatccc ttcaacatga taagatttga cttccgtgcc gccaccgaga
    10321 attgagcctt cgggatctgc tgcaatgatt ttgatggcgg ggttaaactc tttaaggcgt
    10381 ttggcaatac ctgtaattgt tccacctgtc cccacgccag caacgaccat atgcaaatct
    10441 tttccgaagt cgtcaataat ttcctgagct gtcccaaaat aatgggcatt ggggttattg
    10501 gggttcgcgt attgatcaag gatatgggag ttagggattt cagcttgtaa ttttttagcc
    10561 agagaaatat gactgtccgg atcattatac gcggcttctg ttggcgttcg ataaatgatg
    10621 gctcccaggc gttcaagcac ggattgcttt tcctggctca ttttttctgg catggtaata
    10681 attactttgt agcctaatac agcgcctgcc agggctatac ctatgccggt gttcccggaa
    10741 gtaggttcga ttaaggtatc tcccggttta attctccctt ccttttctgc ttttactacc
    10801 atttcatagc caatacgatc ctttacggag ccgccaggat taaaaaattc gcatttggca
    10861 tacagctcgc attccagatc ttttccaagc ctgttaattt ttacaacggg agtatggcct
    10921 atagtggcca ggatgttagg gtaaatcatg gtagtccttt gtcatggtga aatgagttga
    10981 ttataagcgg ataaggtaaa atagcaaaat ggtggtttct acaatcaagt tattttctct
    11041 tattaacgtg gtttatagat caggatcaca taagaagcag tcgatatcca gtatccgttt
    11101 gggctgagga gatgcgcaag catcgtctcg aagccttagt accaaacatt aaattccata
    11161 caaggcttca agacagcata aatgcttcgt cagcccgaac gatacaagat aatgacctat
    11221 cttaatccat aactcacgct tgttggaaag agatgcaaat catcttggca gtttatattt
    11281 gtgatattct cctttaatgc agggatattt cgtttttatg gttatttctc cttttttaca
    11341 ttatatttat tgtgggcgtt ggttattttt gtaaaaacag aaaaacatta cttattaatt
    11401 gagatcaaaa aacggatttc tatctgtttt acaaaaatga caaaaatcca aagtgttttc
    11461 aaatagttac aataattcat ttcaattgat attttgttta attaacgaat gcaggaagct
    11521 taattatgat aaattacaaa acaataaaat ggagatatgg cctcattgcg ctctttgctt
    11581 taggggtatc aggatgttat tttgacgggg caagccctca agaaagagga tattcttacc
    11641 aggaaaatag catgccatcc agtggaggac aaaccaccgc tagcaatcaa tcccagccgc
    11701 aacaaaaaag ctatgcgtca aaggatacct ctcaaaaatc tactcctgga ccaaagcgaa
    11761 cagctgcacc tcaactcccg gttattcaat aaaaagagtt ttacttatta tccctcataa
    11821 tcaacttata tgagggataa ctgaaacttg ttcaaaagat tttctcatag gcatgaaatg
    11881 ccatgatcct tgatggttat caaagatgac agtagagaaa ttcctccttg ttttagttag
    11941 atccataatg ttattttttt ctgatatttg cgtgccttga aaaattattt tagaatggcg
    12001 acggtttagt agtatgaaat acaggagtag atgatggata aattagattg ggagttgcca
    12061 gaggtttgtg caatacctat tgattcagta actgctgatc aagatggaaa tctaaagagc
    12121 gacggtcctg atagtagcgg actttcctca agtagcagtt cttcgggcgg ttaaaaagaa
    12181 aaaaattctt tataagaagc ctacaaatta tttttttcac attattttgg attcgccagt
    12241 aatcaaatgc ctttaggata gttaatggaa tgtggcaacc ttattgatat tttaaacaca
    12301 gaaaagacag ctttgacatc attttcaact catgaaatcc taaagacagc gcaaagaatg
    12361 cgcctgacca attcggttta taccatttta agagggcact atggtattcc ggtttcgcaa
    12421 ctcgcttgtt ttaagcctgt tttagagcaa aatagagaca gggccattag aaaattaatt
    12481 ttgtctatca aaatcaacga gctttttcag caatataata ttccggttat tcatctgaaa
    12541 ggactgccgc tctcgcaaaa attgtttgga gaccctttga ttcgacagtc atgcgacatc
    12601 gatttattca tttctcctca gcatttagaa cgggcagatg aagtgttttt tgaaaatgga
    12661 atcattcggg ataaatacag tttcttacat gatgttaata agctaaagaa acatcgagtg
    12721 ctaaaagatg tttcctacgt ggctgaaaaa tatggtatta gaatcgaatt gcactggcgt
    12781 ttatttagat ggaaaaataa attcaatcag ccttttgagt tttattggaa caataaagtg
    12841 ccgattttgg tgggggggaa aaatttattt tataccctgt cccctattga cgagtatctg
    12901 tacgttgctg tacatggggc gaatcatgga tttgaatctt tgcactggct ttatgatttt
    12961 gccagatatt ccaccttgct aagcatggat gacaggagaa gagttgttga attggttgat
    13021 agtctttcca tacagacaga gtatcaaatg gcgacttatt gtgcggagag aatatttcca
    13081 aatgtattta cagaaagcgg taatgcaggt ttactgccca attccttaac gagaaaagcc
    13141 ttgatttcct taatatacca ttcaaaaagc tcatatcgct cgaaaagaag gatgttacgc
    13201 atggtggcta at
//
LOCUS       DATNSN010000094        13100 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.094, whole genome shotgun sequence.
ACCESSION   DATNSN010000094 DATNSN010000000
VERSION     DATNSN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.094"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1064
                     /locus_tag="VAH76_002078"
     CDS             <1..1064
                     /locus_tag="VAH76_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1386686.1"
                     /translation="VSEPSGYLFEKSLILGFLLREWFPTQLVNNSEVKMEMLEGEKGV
                     CSAFKNYKEYRSFMPKEELKTTEFGVLYEANEAFLEYFYNRSENTLINKDSPFEKYFV
                     DSSSDEEAIKNYWDAEGYTLYCQHLAKPQVKLSYIEIMTMMRVINQPLTIYDRSTSSV
                     VAEYVNPKVNLPDFEVAIDALQGHYFLLKTEETEKELEEYERSHAQYKKDRSEILAHS
                     DKPVSSLLVRATCPKGHLDEDPFVALIESLSEINSLSQIDTNLKNENTDTPNCNFLLK
                     VGASVVGTSSALITLMGIAILSGQYDPGNSLAEEALATTVVSSIVGLGYGLYSFFSSS
                     PKLSELDSTNGLSQKLKMN"
     gene            1256..1675
                     /locus_tag="VAH76_002079"
     CDS             1256..1675
                     /locus_tag="VAH76_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEO1386687.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFMPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            1675..3585
                     /locus_tag="VAH76_002080"
     CDS             1675..3585
                     /locus_tag="VAH76_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEO1386688.1"
                     /translation="MLLIEIVLTIHCLLLVSSLFIGRAWLANKPSFKLKLLRFLLASC
                     VISPIIVHCFNFDQKPERQNYVSFDVLQQYVKQPIFKTESSKFSHESSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLAEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREITCTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSMVKE
                     VDISHLDPRMQQIAERQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSYETKNYDCHSPYYIAGKTFTNYSSNVESASLAEA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDTSTWWQADRSDGLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETTNYSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGHFSKNGKTLVLGGELAAPVFR
                     NVAMDALSSVNQ"
     gene            3635..4435
                     /gene="blaOXA"
                     /locus_tag="VAH76_002081"
     CDS             3635..4435
                     /gene="blaOXA"
                     /locus_tag="VAH76_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEO1386689.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4495..5370)
                     /locus_tag="VAH76_002082"
     CDS             complement(4495..5370)
                     /locus_tag="VAH76_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEO1386690.1"
                     /translation="MDKSPKLMHLLIYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNYQKKTYDAFDMNQILEQLMGADLQKNNANQVLLAQ
                     TIDKYVSQTPLLYFTTKGHAYLAYQETPGTISDNIITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGAQGRAPDLGMVMSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINNQ"
     gene            5737..7197
                     /locus_tag="VAH76_002083"
     CDS             5737..7197
                     /locus_tag="VAH76_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO1386691.1"
                     /translation="MNHKPIQFKDLSLIYPHKTCFQDFSGEIHFADRIALIGRNGSGK
                     STLLKMLCGLFLAPAGEIKIPHDVRFGHLPQVLEDCPDLSGGQRLNRVLSKILSDEPN
                     VLLLDEPTNHLDSRNRRSLMRMLEHYHGTLIIASHDTELINTVTDTLWHIDLGQVTVF
                     RGIYFNYQQILEDKKASIEQELSRIARQKKESHLALMKEQERNKRARVQGEKKIAQRK
                     WPTVRSHTKLGSAITTGNKRLSHINYKKQQLLEELSSLNFPEVIKPKFRLKGLGHHKP
                     LIRIQDASVAYEAVVILEDIHFHLSGCERVALHGNNASGKSTFVKAILGESQIKRTGE
                     WAVPDCNTIGYIDQHYQHLNFNETVLDLMRSSMSHASHTEIRAHLNDFLFRKNEEVEI
                     KVKNLSGGEKARLSMALIAANPPKLLILDEITNNVDLETRTHIIQVLREFQGAMLVIS
                     HDYDFLESIHIETRYQIHQGKIHHFNEKQLEVVHHD"
     gene            7190..8971
                     /locus_tag="VAH76_002084"
     CDS             7190..8971
                     /locus_tag="VAH76_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1386692.1"
                     /translation="MTNRLPNHLGSFMWHFLKPYRGIVILFILFALLAGFWGPFNSLL
                     IKSFINTLAEKTSQGLSSLYWIAGLLVLNFIVFDNITWRTLGYLNYKYEAVIKNQIIS
                     QTFEYVLGGSAQFFQDNLSGRIADQITTLADNLEIILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVKRMNTFFRLVQQAFQRKELFIVLLCCAQGGMIAVMMGLASITLIYLYGK
                     GLVNIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKARQSLNALVIPHDIKDKINA
                     SQLVVTQGRIEFSKVKFHYQGGYSLFQNKSVTIESGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVDGQILIDGQNLAEVTQESLRQAIAMIPQDPTLFHRSLMDNIRYGRTDATDEEV
                     ILASRKAHAHDFISLLPEGYKTLVGERGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NHGGLYKTLWDAQVGGFLPDQQSEKGE"
     gene            8975..9931
                     /locus_tag="VAH76_002085"
     CDS             8975..9931
                     /locus_tag="VAH76_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386693.1"
                     /translation="MSVKQEQYLIKVVSYDSNWPVQFEQEAERIKKALGSNCIEIHHI
                     GSTSVPDLVAKPVIDMIPVVLDISKVDGANAAMRALGYEAKGEYGIPFRRYFQKGDNR
                     RTHHAHIFELGNSEIDRHLKFRDWMRVHPKDREAYARLKQELARQYSDDITAYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFDKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYINMPFDDPDGYEGDPRDTAVGKIL"
     gene            9928..10455
                     /locus_tag="VAH76_002086"
     CDS             9928..10455
                     /locus_tag="VAH76_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386694.1"
                     /translation="MIQMEIVIKTLELSDIPILVDAFQKVNWEKPASLFEAYYQEQRK
                     SLRIVWLAYSQDQIAGYVTLKWISQYQPFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAAGESDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPDGLGVTYGYKPTVPGQTY
                     PLDDDFILWFTKKLR"
     gene            10521..11273
                     /locus_tag="VAH76_002087"
     CDS             10521..11273
                     /locus_tag="VAH76_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO1386695.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQDDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLRGKHKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMNNYKKKISQWTQAD
                     KDRFIQEGKDINQALISAINNQLKPASPEVQSIIHRHHAWVGWNPNKEGYIGLSQRYL
                     TPEFRKFYERQHPDLLEFIIEGMLFYANKNLD"
     gene            11525..12472
                     /locus_tag="VAH76_002088"
     CDS             11525..12472
                     /locus_tag="VAH76_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO1386696.1"
                     /translation="MTQWFVKDLSQLTGVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQAQVLEQKASTLLEGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEIKNNLKQDPGSKIGIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPEIVSWMDKAMDAYWRDRIYGILDQVGKVPSPSVLALWNEM
                     LVDMYGEETSRKNAIYEIALNDDQVSPEAKAWLRNLLDS"
     gene            12672..13097
                     /locus_tag="VAH76_002089"
     CDS             12672..13097
                     /locus_tag="VAH76_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386697.1"
                     /translation="MNYQLELVVDPKLLEQQDKIIRDGIINFNAPFTGAKPERYSIYV
                     KDNDGHIIGGSIVYAHKNSIYVDVLWVSEKYRGMGIGAELLRSIEAEAIKRGISESTL
                     DTFSFQAEGFYLKQGYKHLGTIKNYLVGHDRIFLRKKLK"
BASE COUNT         4112 a         2260 c         2675 g         4053 t
ORIGIN      
        1 atgtttctga accatctggt tatctctttg aaaagagctt gattttagga tttctgctta
       61 gggaatggtt cccaactcag cttgttaata attcagaagt taaaatggaa atgctggaag
      121 gtgagaaagg agtatgcagt gcttttaaaa attataaaga gtatcgctca tttatgccca
      181 aggaagaatt aaaaacaaca gaatttggtg tgttatatga agcgaacgag gcttttttag
      241 agtattttta taatcgctct gaaaatactt taataaataa ggattcacct tttgaaaaat
      301 attttgttga ctcgtcctca gatgaagagg caattaaaaa ttattgggat gcagaagggt
      361 atacattata ttgtcaacat ttggctaaac ctcaggttaa attatcttat attgaaatta
      421 tgaccatgat gagggtcatt aatcagccac ttacaatata tgatcgttct acctcctcag
      481 ttgtagccga gtatgtcaac ccaaaggtca atttacctga ttttgaagtg gcaattgatg
      541 ctcttcaagg gcattatttt cttttaaaaa ctgaggagac ggaaaaagag ttagaagaat
      601 atgaaagaag tcatgcccag tataaaaaag atagaagtga aatccttgca cactccgata
      661 agcctgtttc ttccttattg gttcgagcta cttgccccaa aggtcatttg gatgaagatc
      721 cttttgttgc attaattgaa agcctttccg aaataaacag ccttagccaa attgatacta
      781 atttgaaaaa cgagaataca gataccccca attgcaactt ccttttaaag gtaggtgctt
      841 ctgtagttgg tacctcttct gcactaataa ctttaatggg gatagccata ttatctggcc
      901 aatatgatcc cggaaacagt ctggctgaag aggcattagc tactactgtt gtatccagta
      961 ttgtgggttt aggttatggg ctttatagct tctttagttc tagccctaag ttatcagagc
     1021 ttgattctac caatggatta tcgcaaaaac ttaaaatgaa ctaacctcct gaattagaaa
     1081 atattgtctg taagccgatc gtgttgatat taggtatcaa cacgattgcg ccattcaagt
     1141 aaactgttct cgcgtgtaga gaagtatttt tcccataact atttcaactc tactattttt
     1201 atagtagaat cctactataa aaatagtaga gtgtatgtgg ttattggaga gaaacatgtc
     1261 ggcaaaaaaa aattcgatag ctcatgatcg acttcttaca gaagtagagc ttgaattaat
     1321 gaatattatc tggagccttg ataaggtaac tattaaggag gttgtttcac atctgcctaa
     1381 ggaaaggcct ttggcttata caaccgtggc aacagtacta aaagtgctgg aacaaaaggg
     1441 atttcttgag tgtcaaaaaa attcctatgc tcatgttttt atgcccatag ttacaaagtc
     1501 agattatgaa aacacttgta ttgatcatat ggttgccaat gtttttgatg gagagccccg
     1561 agcacttgtc caaagacttt tattagccag aaagttacag catgatgaca ttcaagccat
     1621 tgaagaagca cttaaacaat taaatgctgg tgaaaaacag ccagaggtga tgtaatgtta
     1681 ttaatagaaa tcgtattgac tattcattgc ctgcttcttg tcagttcact atttataggg
     1741 cgtgcctggt tagcaaataa accatctttt aaactgaaat tactccgttt tttattggcc
     1801 agttgcgtta tttcaccaat aatagttcat tgttttaatt ttgatcaaaa gcctgaacga
     1861 caaaactatg tatcatttga tgttttgcaa cagtatgtaa agcaacccat atttaaaaca
     1921 gaatcttcaa aatttagtca tgaatcgtct tctgccttta catcgatgaa tgttaactac
     1981 tgtcaactct taagcgtgat gtttttttta atcgtcttgt ttcgtagtta tcacttgtta
     2041 tcaggtttag gtcgcttgaa attgatgttg gctgaagcaa tcccatatcg aacctccgga
     2101 aaactagtta ttaaggtttc tcaccactgc catgttcctt tctcaatatt gctatttaat
     2161 aaagcctaca tcgtattgcc agtatccata ttttcttctt ccatgaatgt gaggattgct
     2221 attgctcatg aagggcaaca ccatcgtaat ggagattgtt catgggccta ttttatcgaa
     2281 atcatacgta ttatcttctt tggaaacccg ggtgtaactc aatggtttcg tgttttagca
     2341 gatttacagg aattgtcatg tgatgaagta ttggttggac atcaaaaaat ttcggcacat
     2401 gactatggtc actgtctatt gcaagtggta gaggcagtat cccaatgctc tctggcatct
     2461 aaccgggaaa ttacatgcac ggtgggtatg gctttgaaca aagaaaatga agactgcaaa
     2521 tttataataa ggagaatttc aatgctaact gcttattcac ccaatgcatc caggcctttg
     2581 ctattaggga tagtctttgc aggattttct atcctgtcac caatctgtgt tgcctattca
     2641 gcagcaggat ctttgacgag ttccatggta aaagaagttg atatttctca tttagatccc
     2701 agaatgcagc agattgcaga gagacaaatt acgacagccg tcaaacaata tcatgcaaaa
     2761 tctggagtta ttgcaatagc tgatccccaa acaggcaata tcattgcatt tgctgaatca
     2821 agcaaaaata aggggttaga gagttggaaa tcacgtattt tttcacccgg atctactata
     2881 aaacctttta ttgcagcagc tgcaatcaac tctggaaata gctatgaaac aaaaaattat
     2941 gattgtcatt caccttatta catagcaggc aaaacattta caaattatag ttctaatgtt
     3001 gaatcagctt ccttagcaga agctatcgca aaatctatca atgtatgcct tatcagagtt
     3061 tctcaagagg ctggagtgcc ggtaatccgt aaaaagctca ctgaatttgg ttttgacaca
     3121 agcacttggt ggcaagcgga tcgaagtgat ggtttacaac tggcaatggc tgcacttggg
     3181 gagaatatcc ctgtcacaat cgaatcgcta ataaaatctt atgccattct tgctaacaaa
     3241 ggacactcat ttgacagagg aaatagtgcc attatttcag aaacaacgaa ttactcaata
     3301 aatcatatgc ttgaaaatgc tgttacaaat ggcactggaa aactcgcagt tattcctggt
     3361 gtttctgtag ctggtaagac aggaactgta attgaaaata atgataaata tcttgctttg
     3421 tttgccgggt atgtgccagc tgataatccg cgttatgttg tattagtcgt gattgaagag
     3481 ggtcatttca gtaaaaatgg aaagacatta gttttgggag gagaattggc cgcaccggta
     3541 ttccgaaatg tcgcaatgga tgctctgagc agtgtaaatc aataactagc ttatgggttt
     3601 gcatatccat atattcaaga atgttgaggt tgttatgaag aaaataatta tatttttgtg
     3661 cacaggatta ttattttgcg tctctacttg ggctcaaggc acctgttttt tagcaaaaga
     3721 aaatcaaact gttttaaaac gtgagggcaa tgattgtgat caacgctatt cgccagcatc
     3781 aacttttaaa attgccctta gcctcatggg ctttgattct ggaatattaa aggatgagtt
     3841 gcatcctgaa tggccataca aaaaagaata tgaactttat ctgaatgtat ggaagtaccc
     3901 tcaaaacccg catacctgga tcagagactc ctgtgtttgg tattcacagg cattaactcg
     3961 ccaattgggc atgaaacggt ttaaagggta tgtcgacgca tttcactatg gtaatcagga
     4021 tgtttcggga gacaaaggtc aaaataacgg tttaacccat gcctggcttt caagctcact
     4081 ttctatctca cccacagagc aaattcaatt tctacaaaaa ataatctata agaaactacc
     4141 ggtgagtcaa aaagcttata ccatgactaa aaacatcatg tacatccagg agttgcctgg
     4201 tgggtggaag ctttatggaa aaacaggaac tggtcgacag ttaacaaaag ataaaagtca
     4261 aaaattgcct ttacaacacg gatggttcgt aggttggatt gaaaaggatg agcgtgtgat
     4321 tacctttgca aaacatattg cagacagtaa agagaatacc acttttgcca gttttagagc
     4381 gaaaaatgat acattgattc aattgtttaa cctgattaat gaactggaga aataatctaa
     4441 aagatggccg gttcagtgtt ttctgatccg gctatctaac tctctgaatt ataatcactg
     4501 gttgttaatt tcgcatttta gtttggatgc ttgtttatta acccagatta aaaatttaat
     4561 gagatcatta atttcttccg ttgagaggtt tttgcctatc ggtggcatgt atcctcgccc
     4621 attctttaca agtttactga tatctgaagg cgttaaataa ctcatcacca tacctaagtc
     4681 tggtgcgcgt ccttgagccc ccttgtaaaa aagagagtgg catgtggagc atatattatt
     4741 aaaaatatga tgaccatttt gcacagaagc agattcattc aatggattta aaaaatgaga
     4801 aaaagtcagc tctttcttat ttactatttg catcgaaata acctggtatg gccaactcac
     4861 agggtaggta cttgtattat cccatacaat atagaaagga ccaggatttt cttttttacc
     4921 gtgttgatta atgtaactcc agcgaccatc tttggtgata atattgtcag agattgttcc
     4981 gggcgtttct tgataagcaa gataggcatg tccctttgtc gtaaaataca acaagggagt
     5041 ttgagacaca tatttatcta ttgtttgagc taataatacc tggtttgcat tatttttttg
     5101 taaatctgct cccatcaatt gctctaaaat ctgattcata tcaaacgcat cgtatgtttt
     5161 tttctgataa ttatatgcag gattatgaga gataatcaca cgaatggttt taaattgttt
     5221 ttttagctcc gacagggtat atgttttatt ggcaatagta attttaagta gtttttctgg
     5281 tgaattgggt tctccaaaac aacatgttga aactaatacc aggtgaaccg cataaatcag
     5341 aagatgcatt aattttgggc tcttgtccat aagtccttct tgtttgttga aaacaaatca
     5401 gatttttccc atactgttac tagctattat ggtttataac aggaccgatt ttccagcgcg
     5461 cagagttatt taacttaaat ctgagacact tataagacgc caatggaact attatgaaaa
     5521 ggaagagata agccaggaaa aataattttg aatataatat tgacaccttc aactatttta
     5581 atttatattt tctgtaattt ttttaccagt taaaaggtag gtttaggccg tcatgaaaat
     5641 tacatcttaa gtacgcccaa ctatagggca aagtgaacaa agtcctttgt gacttttagt
     5701 ttttactgtc ctggcagaca tgcttaagag taaatcatga accataaacc gattcaattt
     5761 aaagacctca gtcttatcta tccgcataaa acctgttttc aagactttag tggtgaaatc
     5821 cattttgctg atcgcattgc actgattggc cgtaatggct caggaaaatc aacgctactc
     5881 aagatgcttt gtggtctttt tttagcccct gctggcgaaa ttaaaatacc ccatgatgtt
     5941 cgctttggtc atttacctca agtgcttgag gattgtcctg atttaagtgg cggtcagcga
     6001 ttaaaccgag tattaagcaa aatactgtcg gatgagccta atgttttgtt gttagatgag
     6061 cccacaaatc atctggacag ccgtaatcgt cgttctttaa tgcgcatgct tgagcattat
     6121 catgggacat tgatcatagc ctctcatgat acagaactca tcaatacagt cacagatact
     6181 ctatggcata ttgatttagg tcaagtgacc gtattcagag gaatttattt taattatcaa
     6241 caaattcttg aagacaagaa agcatcgatt gaacaggagt tatcaaggat tgcacgacag
     6301 aaaaaagaat cacatcttgc tttgatgaaa gagcaggagc gtaataaacg tgcgcgtgtc
     6361 cagggtgaaa agaagatagc ccaacgcaaa tggccaacgg ttcgatctca tacaaagctc
     6421 ggttcagcaa ttacaacagg caataaacga ttaagtcaca ttaactataa aaaacaacaa
     6481 ttactcgagg agctttcctc tctaaacttt ccggaggtaa ttaagcccaa atttaggtta
     6541 aagggacttg ggcaccataa acccttaatc aggattcaag acgcttctgt tgcctatgag
     6601 gctgttgtga ttcttgaaga tattcatttt caccttagcg gttgcgaacg agtggcatta
     6661 catggcaaca atgcttcagg taagtcgacc tttgtcaaag cgatcttggg ggaaagtcaa
     6721 attaagagaa caggggaatg ggctgtgcct gattgcaata caataggcta tatcgatcaa
     6781 cattatcaac atttgaactt caatgaaaca gtcctggatt taatgcgctc cagtatgtcc
     6841 catgcatctc atacagaaat tcgtgctcat ttaaatgatt ttttatttcg caaaaatgaa
     6901 gaagtagaaa ttaaggtcaa aaatctttct ggtggtgaaa aggcaaggtt atcgatggca
     6961 ttgattgctg caaatccacc taaactactt attttagatg agatcactaa caatgtagat
     7021 ttggaaacac gcactcatat cattcaggtg ctgcgtgaat ttcaaggtgc aatgctggtt
     7081 atttctcatg actatgactt tttagaatcc attcacattg aaaccaggta tcagattcat
     7141 caagggaaga tacatcattt caatgagaaa caattagagg tagtgcatca tgactaatcg
     7201 tttacctaat cacttaggct cttttatgtg gcatttttta aaaccatatc gaggcatagt
     7261 gatattattt atcctgtttg ccctattggc cgggttttgg gggccattta atagcttatt
     7321 gattaagtct ttcattaata ctttggctga aaaaacaagc cagggcctat cttctttgta
     7381 ttggattgct ggattattgg tattgaactt tattgttttt gacaatataa cctggcgaac
     7441 acttgggtac ctgaactata aatatgaagc ggtcattaaa aaccaaatca ttagccaaac
     7501 atttgagtat gtgttaggag gaagcgcgca attttttcag gataatctat ccgggcgaat
     7561 tgccgatcaa atcacgaccc ttgctgataa tcttgagatt attttgcatc gtgtgtctgt
     7621 agattttctt cgtggcgctt cattattagt ggtttcattt attacagcct attttgttaa
     7681 tgtcttgttc ttttatattt tgtttctctg gttcgtcgcc tttgcctcat ttagtatttg
     7741 gatgtcagcg cgattagtcc agttgtctga tgatcacgca agttctgaat cacagctttc
     7801 cgggcagttg gtagatagcc tggcgaatca atccaacatt cgtatctttt cacgaaaaat
     7861 ctacgaagtg aaacgtatga acactttctt tcgcttggta caacaggctt ttcaaagaaa
     7921 agagcttttt attgttttgt tatgttgtgc acaaggagga atgattgcag taatgatggg
     7981 tttggcatcg attactttaa tctatttgta tggtaaaggt cttgtgaaca ttggtgattt
     8041 tgccttgatt ctcggactat ccatggaatt gggccatatg atgtggtaca ccatgtacca
     8101 agtggatcaa tttaaccaag ccttaggcaa agcaagacaa agtttgaatg cattggtgat
     8161 acctcatgac attaaagata aaattaacgc atctcaatta gtagttaccc aaggacgaat
     8221 tgaattttcc aaggtaaaat ttcattacca gggcggttat tctctgttcc aaaataaatc
     8281 tgtaacgatt gaatcaggtc aaaaagtggg tttagtaggc tattcaggca gcggtaaatc
     8341 gacttttgtg aatctgattt tacgacttta tgaggttgtg gatggccaga ttctcattga
     8401 tggtcaaaac ctggccgaag tcacgcaaga aagtttaagg caagctattg ctatgattcc
     8461 tcaagaccca actctttttc atcgaagctt aatggacaac atacgctatg gcagaacgga
     8521 tgcaacggat gaggaagtga tactagcctc caggaaagct catgcacatg acttcataag
     8581 tcttttgcca gaaggctata aaaccctggt aggggagcgt ggggttaaac tttcaggtgg
     8641 tcagcgccag cgcattgcga ttgcccgagc catcttaaaa aatgctccga ttttaatgct
     8701 ggatgaagcc acgtctcagc ttgattccat taccgaatcc aatattcaag agagtctctg
     8761 ggatttaatg cagggaaaaa caaccctggt ggttgctcat cgcttgtcta cccttctgca
     8821 tatggaccga attttagtct tcgataaagg ccatattatt gaagacggta cgcatactga
     8881 actattgaat catggtggtt tgtacaaaac tttgtgggat gcccaagttg gtggtttttt
     8941 gccagatcaa cagagtgaaa aaggagaata agcaatgtca gtcaagcaag agcagtatct
     9001 cattaaagtg gtttcttatg attcgaattg gcctgtgcaa tttgaacagg aagcagagcg
     9061 gattaaaaaa gcattaggca gtaattgcat tgaaattcat catattggtt ccacttcagt
     9121 acctgatttg gttgcaaaac cagtcatcga tatgattcca gttgttttag atattagcaa
     9181 agtagatgga gctaatgctg ccatgcgagc acttggctat gaagcaaaag gagaatacgg
     9241 tattcccttt cgccgttatt tccaaaaggg agataatcga cgaacgcatc atgctcatat
     9301 ttttgaactt ggaaactctg aaatagatcg gcatttaaag tttagggatt ggatgagagt
     9361 gcacccgaaa gatagagaag cttatgcacg tttaaaacaa gagttggcgc gccaatattc
     9421 tgatgacatt accgcctatt gcctgggtaa agaagatttt attgcaacaa ttgataaaaa
     9481 agccgggttt aatggattaa gaatggttaa ggcactaaca acgcgtgaat gggataaggt
     9541 gcgtcatttc agacaatttt atttttttga caaagcaggg ctttctgatc cttatacctg
     9601 gacttttgag cataatgcgc atgttcattt tgttttattt caaggaagtg aaatcacagg
     9661 ttatgctcat ctgcaattat ggtctcataa cagagcggca ctacggatta tcgttatcga
     9721 tgaacttaaa agaaaccatc aatatggtac ccgttttttg gcattatgtg aaaaatggct
     9781 caaaagtcag ggctatcaga gtttgcatgt cgagtcgtca ccagatgctc tgaaatttta
     9841 tcgaaataat ggttatatca atatgccttt tgatgatccc gatggctatg aaggtgatcc
     9901 acgggatact gcagtaggaa aaatattatg attcaaatgg aaattgtcat taaaacactg
     9961 gagctatccg acatccccat tctggttgat gcatttcaga aagtaaattg ggaaaaaccc
    10021 gcttcattgt ttgaagccta ttaccaagaa cagcgaaagt ctcttcggat agtatggttg
    10081 gcttattctc aagaccaaat tgctggttac gttactctaa aatggatatc gcaatatcag
    10141 ccgttcgccc atcaaaaaat tccagaaatt atggatttaa acgtattgcc ctcttttcgt
    10201 aaacagggag tggggactgc tttactgcaa gccgctgaag aaaaagcagc gggcgaaagt
    10261 gatgtcgtgg ggattggcgt tggtttatat ggcggccatg atggaggata tggacaagct
    10321 caacggcttt atgtcaatcg gggttatatc cctgatggtt taggtgtaac ttatggttat
    10381 aagccaactg tcccagggca gacctatcct ttagatgatg atttcatttt atggtttaca
    10441 aaaaaattaa gatgaaatgc ttgaccctta cgttacgtga gcacttatct ttaaatggaa
    10501 tcatttgaga ggacagttta atggcttata cggtaaaaca attagcaaaa atatcgaatg
    10561 taagtgtgcg cactttacat tggtatgatg aaataggctt attaaaacca gcctatcaag
    10621 gtgctaatgg ctatcgttat tatgaagaaa agcagtgctt aaaattacag caaattttat
    10681 tcttcagaga gctgggattc cccttgaatg atattcaaaa actgcttatg caagatgatt
    10741 ttgaccatat caaagcattg agagcgcata aacaggtttt ggaaagcgat atcaatcgaa
    10801 aaaatcaatt gattgtaacg attgataaaa ccattcaaca tttaagaggt aagcataaga
    10861 tgtctaatga agaattatat tatggttttg attcactgcg acaaaaacaa tacgaaaagg
    10921 aattagttaa agaaggggtg gtcagccagg attggatgaa taactacaag aaaaaaatta
    10981 gtcaatggac tcaggctgat aaagaccgat ttattcagga aggtaaagac atcaatcaag
    11041 cactgatttc ggctattaat aatcaactta agcctgcttc tccagaagta caatccatta
    11101 tccataggca tcatgcttgg gtaggctgga acccaaacaa ggaagggtat attgggctaa
    11161 gccaaagata tttgactcct gagtttagaa agttttatga gcggcagcat cctgatctgc
    11221 tcgaattcat tattgaaggg atgctgtttt atgcaaacaa gaaccttgat taagctgaga
    11281 gaagggctgt gtcttatcag ccctaaatga tattggattg ggagcattgt gcgtgttagc
    11341 agaaataata ctgatttata catgccagct tatcatgttg ttccaaacca ggagcttttg
    11401 tgagtttttt gcttaatcat tgatctcttg gtaggttaga aaaataatgg ctcaagaaat
    11461 tttaaaaact gcttgaccct attgttgcta taggcattac ctttgtcttg aagggggaaa
    11521 cacaatgaca caatggttcg taaaagattt aagccaatta actggtgtat cggtacaaac
    11581 gttacatcac tatgaccgta taggattgct aaagccttca ttacgacttg ccaatgggta
    11641 ccgtgtttac tctgagaagg atttattgaa attacaacag attattgctt tgaagttttt
    11701 cggttttgag ttgtcacaaa tcaaaacctt actcgctgaa gaaggtaatg cgctcaatca
    11761 ttttaacagc caagctcagg tgttagaaca aaaagcatcc actttacttg agggagctaa
    11821 aactttgaga cgcatcattg actctgttga caaaaatcaa tctatccctt gggaaaccat
    11881 tattcaactt atagaggtat ataaaatgac agaacatctt gagcatggct gggtaaagga
    11941 aatattcacg ccggatgaat taaaacagta cgctgagttt gaaaaagaaa tgaaagccaa
    12001 tgccacgcct gagcaaaaag cggctatgga aaacaattgg tctcagctag ttgatgagat
    12061 aaaaaataat ctgaagcaag atccaggctc aaaaatagga atttatttag gaaagaagat
    12121 tatggattgg gttaatgggg tctacggtaa aaaatatgcc catttaagga ccaaaaaatt
    12181 tgaacagggt tttggggaag gaaaaggtct tgatgaggtt ggattaacac ctgaaatcgt
    12241 atcctggatg gataaagcga tggatgctta ctggcgagac agaatctatg ggattcttga
    12301 tcaagtagga aaagttcctt caccaagcgt attagctctt tggaatgaga tgctggttga
    12361 tatgtatggt gaggaaacat cgcgtaaaaa cgccatatat gaaattgctt taaatgatga
    12421 tcaggttagt cctgaggcaa aagcctggct tagaaatctt ttagattcct aaaaatatga
    12481 ataacactgt tggatcggta ttgttttacc gatccattcg actcgatttg ataccttcaa
    12541 ttattaattg atttagcatc ttaggcagac caatcgtctc taaataaatt taattgctat
    12601 agacacaata aaccatgatt ttcaaacttc tgtttaatgc cataatttaa tattttaagg
    12661 aatagttata aatgaattac caattagaac ttgttgtaga tccaaagctt ttagaacaac
    12721 aagataaaat tattcgtgat ggaataataa attttaacgc tccttttaca ggagccaagc
    12781 cagagcgtta ttcaatctat gttaaagata atgacggcca tatcattggc ggatctattg
    12841 tatatgctca caagaattca atctacgtcg atgtcctctg ggttagcgag aaataccgtg
    12901 gcatgggtat cggagctgag cttttacgaa gtattgaagc ggaagcaatt aagagaggaa
    12961 tttcagagtc aacattggat actttttctt ttcaggctga aggcttttat ttaaaacaag
    13021 gttataagca tttggggact ataaaaaatt atttagtagg acacgatcgg atttttttgc
    13081 gcaaaaaatt gaagtagtcg
//
LOCUS       DATNSN010000095        13046 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.095, whole genome shotgun sequence.
ACCESSION   DATNSN010000095 DATNSN010000000
VERSION     DATNSN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.095"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..858
                     /locus_tag="VAH76_002090"
     CDS             <1..858
                     /locus_tag="VAH76_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEO1386698.1"
                     /translation="QWLENLRSKATTYSGQSFCDSLINLLEQSSGFFDDTGKIKPKGL
                     RVLLRFIPVVFASYGTFLFAEELFAIYALYFILLKGGQYLESTESSQLKKIGCTLQEI
                     SIITATASTTLLVRLLEMTFWASRQFLDVSLQIGSAILKPMLPAPETKEKSESDTAAN
                     LCRDLILASHNTGEGMQFKTPELKVISAPLESYLGLNAQQFFGDLRIGREKRLKVEAF
                     LFKMRVLDSLPSPLEEKLVEAQKELDKIKKDAKVFTSNTAKAVKEAEQVISLLQDQDS
                     MQLVIYNGT"
     gene            1266..2015
                     /locus_tag="VAH76_002091"
     CDS             1266..2015
                     /locus_tag="VAH76_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEO1386699.1"
                     /translation="MKILVAVKRVIDPYVKIRVKSDNSGVETQNIKMAMNPFDEIAVE
                     EALRLREKNWATEVIAVSIGSDSSQETLRHALALGADKAILVRTEKSFESLNIAKILN
                     SIVANEKPDLVLMGKQSIDGDNNQTPQMLAALLNWPQATNASKIIPDNNNLEVVREID
                     GGLETLKVQLPAVISTDLRLNEPRYASLPNIMKAKRKPLDIIDLDSLGLSLKQHVEIL
                     KVNAPATRSGGVILDSVAALLDKLQHEAKVL"
     gene            2029..2967
                     /locus_tag="VAH76_002092"
     CDS             2029..2967
                     /locus_tag="VAH76_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEO1386700.1"
                     /translation="MSTLVLVEHNNQQLHPATRNTLSAALELGDKITLLVVGYQCRSV
                     AEEAAILKGVHAVLHVDNPCYEHQLTEAVSDLVVSFANSFSAILAPSSTYGKNILPRV
                     AAQLDVTQISDVSKIINSDTFEHPVYAGNAIETVRVLDSLKVMTIRTTAFDSITSSQE
                     ACCIESIDKEFQAKGSQFVRHELSKSERPDLGSAKIVVSGGRGLQSAEKFKLIEELAD
                     TLGAAVGASRAAVDAGFVPNDYQVGQTGKVVAPMLYIAVGISGAVQHLAGMKDSKVIV
                     AINKDADAPIFQIATYGLVGDLFELVPQLIEQLKNR"
     gene            2979..4100
                     /gene="ald"
                     /locus_tag="VAH76_002093"
     CDS             2979..4100
                     /gene="ald"
                     /locus_tag="VAH76_002093"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEO1386701.1"
                     /translation="MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIG
                     ISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPH
                     QARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
                     GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTV
                     YSTADSIEKYVSSADLVIGAVLVPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFE
                     TSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPFILSIVTKGVKLALL
                     SDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR"
     gene            complement(4173..6557)
                     /locus_tag="VAH76_002094"
     CDS             complement(4173..6557)
                     /locus_tag="VAH76_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEO1386702.1"
                     /translation="MKKMAYFWRKGLWALMSLFFLLIVAGSLLYLYLESQLPNVDSLK
                     TVQLQVPLQIFSKEGLLIQEYGEKKRIPVTYDEIPPTLIHALIATEDQRFFEHPGVDV
                     MGLGRAAVSMLKTGTKSQGGSTITMQVARNFFLSRKKTFLRKFNEIMLAIKIDRELSK
                     EKILELYLNRVYLGNRAYGVGAAAMVYFGKSLKDLNLAELAMIAGLPQAPSTQNPIAN
                     PLAAKKRRDHVLERLLEEHYINEEQYQNAVNQPITAKYHGTNIKVEAPYVAEMIRQSL
                     YDNFGPEAYTKGYKVYTTIDGKLQNTANEVVEKNLIAYDHRHGYRGPIANIGEKDSQS
                     PKIRQKYLRQYPELNSLIPAVVTEVREKEATAALQNGQIIIIPWEGMSWARPALKRGG
                     WVGKSPTKAQQVVAAGDIIYVHPTEKSWQLAQIPEAESAMVAMNPKNGAIEVLVGGFN
                     FSKSKFNRATQSSRQPGSSFKPFVYAAALNNGYNLATLINDAPIVVDDPSQPNLWRPH
                     NVNLKFNGPTRLKQALVQSKNLVSIRILDDIGIDYTIDFLTRFGFNKKSLPRALSLAL
                     GSLSISPMDLTTAYAVFANGGYKVEPYLIDHITDADGKILLQAKPSVVCDPCDKDKID
                     ASTLAPRVISEDIAFLMNTALRDVIQHGTGRAARVLNRQDIAGKTGTTNDQVDAWFAG
                     FNPNLVVTTWIGFDNPKSLHEYAANLALPLWIDFMKVALKDKPETELKQPENVIAVRI
                     DPVSGLLAKPNQENGIIEYFRQNEVPSEEDQGPVYNAHNDQQEEELAPVEESLF"
     gene            complement(6711..7718)
                     /locus_tag="VAH76_002095"
     CDS             complement(6711..7718)
                     /locus_tag="VAH76_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386703.1"
                     /translation="MTRLEELLYTLTSVIIRYHDSQPKVLNKLIVESNPILLRRKALA
                     LAKEIIQDKDVECKGRLEELIQNCTKGYPDRKPFLYFILNEVLFLKSMLDRKSSFEPS
                     KLEEYKKQIAQLLIDLKQLMITPKSKTYEVKYTSATENSETSISLSGVKNDGYVGGEF
                     CNSGNLIKEEVLEALNIHINSKNTAECSSDEEIKALAESLCLEHQNALLVPELLEKAR
                     ILTESSQEQENELLTKTEQTKTDKKIILEQQATIQEKDKEIEKLKEKVSELTRSMPRP
                     VFNPVLPLGYSSLFFNTKLGTQAKQQTMLDVTDLPMVKSSETTDTQPNQPFSMGNGNG
                     D"
     gene            8135..9199
                     /locus_tag="VAH76_002096"
     CDS             8135..9199
                     /locus_tag="VAH76_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEO1386704.1"
                     /translation="MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEI
                     PASALEGNMIKDVDVVANCIKKLVERLRFTSKNVALAVPDSAVISKVVQINEGLTDLE
                     MEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
                     AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIVAIVDIGANYTHLFVLHGMRLIFS
                     REEKFGGMQLVEAIAEHYHMNLEQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTL
                     QFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANPLSHMSFGKKVNLDV
                     INNDAPTLMVACGLALRHVE"
     gene            9203..9751
                     /locus_tag="VAH76_002097"
     CDS             9203..9751
                     /locus_tag="VAH76_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEO1386705.1"
                     /translation="MTEINLLPWREQRREQEKKLFTTMLLIGIIIAAAIVFLMNSYVS
                     HLINNQMNRNQILQKEITALDEQIKEIKALKQIREGLISRMSIVQNLQSTRTLMVHLF
                     DELIKITPPGVYITKMQREHDIVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
                     DVQKKQSPNNEFKLSFVLKPKY"
     gene            9765..10364
                     /locus_tag="VAH76_002098"
     CDS             9765..10364
                     /locus_tag="VAH76_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEO1386706.1"
                     /translation="MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKP
                     NFEQLEIQEAQEVALKSEFEKKQHQAANLQAYRNQLQLMNERFGTMLKQLPAKNEMPG
                     LLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
                     IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ"
     gene            10361..10945
                     /locus_tag="VAH76_002099"
     CDS             10361..10945
                     /locus_tag="VAH76_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEO1386707.1"
                     /translation="MIIKNNQTLILGFFGNSLRVSLTAATFRKSLLTGLLVLLLSACS
                     GDSSDLVKYINEVKSRPGRPIEPIPKFAPLPIFRFPENDNRRNPFKPIDQKKRNDIYA
                     PDKKRPKQPLESFPLDALKFVGTLKKDNEIWALIKQPDGQISRVRVGDYMGQNYGRIV
                     LIKNDLIKLEETVQKSGTWEKQSTTINLDTGKQE"
     gene            10949..13045
                     /locus_tag="VAH76_002100"
     CDS             10949..13045
                     /locus_tag="VAH76_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEO1386708.1"
                     /translation="MRKIIAVLILISASLSMAYPKDNSLISVKVLPLPENRVRIDFQF
                     SKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRA
                     ILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
                     EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTRIPLNLMKRYDVSDFHSPVQIITM
                     QQEGKKVRMTILSKGDYGHFVYQVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLN
                     FQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQALDIILTTQGLDKRRK
                     GNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSL
                     LSDRGKISVDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQD
                     IGIRWGVSRPTHLSGTLEGANELARGTAPANVVPLERRLNLDLAAAPLTGATPASVGI
                     ALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQEATSSGA
                     TAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVN
                     NGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITN
                     ALSITTIEGREKDVYK"
BASE COUNT         3979 a         2272 c         2788 g         4007 t
ORIGIN      
        1 caatggctgg aaaatttaag atccaaggca actacctatt ctggccagag cttttgtgac
       61 agtttaatta atttattaga acaatccagt ggtttttttg atgatactgg aaaaataaaa
      121 cctaaaggac tcagagtttt acttcgattt attccagtag tatttgccag ctatggcact
      181 tttctttttg cagaagaatt gtttgctatc tatgcgcttt attttatttt attgaaagga
      241 gggcagtacc tggagagcac tgaatccagt caattaaaaa aaataggctg cacgttgcaa
      301 gaaatcagta ttattactgc aactgcatca actactctat tagttcgcct gcttgaaatg
      361 actttttggg cttcgcgcca atttcttgat gtgagtttac aaatcggctc tgcgatttta
      421 aaacctatgt tacccgctcc tgagactaag gaaaaatcag agtctgatac agctgccaat
      481 ttatgtagag atttaatctt ggctagccat aatactggtg agggaatgca attcaagaca
      541 ccggagttaa aagtcatttc tgctcctctt gaatcttatc ttggtttaaa cgcgcaacaa
      601 ttttttggtg acttgaggat aggtcgagag aaacgtttaa aagtagaggc atttttattc
      661 aagatgagag tattggattc tcttcctagt cctttggagg agaaattggt agaagctcaa
      721 aaagaactgg ataagataaa gaaggatgcc aaggttttta caagcaatac agcaaaagcc
      781 gtaaaagaag cagaacaagt tatctctctg ctacaagacc aggattctat gcaattggtg
      841 atttataatg gaacttaata gctgttgcaa gtatctcatg cttattctca ggacaaaata
      901 acacattggt taattaatga attatgtgtg tgatttgttc atattcgtga agctattgca
      961 aattttgctt ttcagtttgt tataaaaata gctccctgcc tgcgcggaaa tgaaaacata
     1021 ccaccattgt ttgcactgct gattgtactt aaggacatta ttttgccctg tgtttattct
     1081 ttttgcatga gttgtgtttt ccaacccgaa ccaggttatc tggcaggtta taagaaaaaa
     1141 aggctttctt taacatagtt caattagaat aaattagctt tatgttctaa ttaaaatcaa
     1201 ccccttgaat ttatacccct atctctggca agatgcagac tgatttgata ttccagtgag
     1261 gaaccatgaa aatactcgtg gctgtaaaac gtgtgattga cccttatgtc aaaattcgag
     1321 tgaaatcgga taattcagga gttgaaactc aaaatatcaa aatggcaatg aacccatttg
     1381 atgaaatagc cgttgaagag gcattgcgtt tgcgagaaaa aaattgggca acggaagtta
     1441 ttgctgtaag cattggttcg gatagctctc aggaaacttt aaggcacgct ttagctttgg
     1501 gtgcggataa ggccattctg gttcgaacag aaaaatcatt cgagagcctt aatatagcca
     1561 agatattaaa cagtatagta gctaatgaaa aaccggattt ggttttgatg ggcaaacaat
     1621 cgatagatgg tgacaataat caaacccctc aaatgttggc tgcattattg aattggccac
     1681 aggctaccaa tgcgtcgaag attattcccg ataataataa tcttgaagtg gtgagagaaa
     1741 ttgatggtgg tttggaaaca ctcaaagtac agctgcctgc agtaatcagt accgatcttc
     1801 gtttaaatga gcccagatac gccagcttgc cgaatattat gaaggccaaa cgaaagcctc
     1861 ttgatatcat tgatctggat tcgcttggtt tatctcttaa acaacacgtt gaaatattaa
     1921 aggttaatgc cccggcaacg agaagtggtg gtgtcatact tgattctgtg gctgcattgt
     1981 tggataaatt acaacatgaa gccaaagtgc tttaattaag gagatgagat gagcacttta
     2041 gttttagttg aacataataa tcagcagtta catccagcaa cccgtaatac actgtctgct
     2101 gctttagaac tcggtgataa aattacgtta ctagtagttg gatatcaatg ccgttcagta
     2161 gccgaggagg cagccattct caaaggagtt catgctgttt tgcatgtaga taatccttgt
     2221 tacgaacatc aattgactga agcagttagc gatttggtgg tgtcttttgc gaattcattc
     2281 agtgctattt tagctccttc cagtacttat gggaaaaata tattgcccag agttgccgca
     2341 caacttgatg tgacccagat ttctgatgtg agcaagataa ttaattcaga cacatttgaa
     2401 catcctgttt atgctggtaa tgccatagag actgtgcgtg ttttggattc attaaaagta
     2461 atgactatta gaacgacagc ttttgattcc atcacatcaa gtcaggaagc atgttgcata
     2521 gaaagtattg ataaggaatt tcaagccaaa ggaagtcaat ttgtgcgcca tgaattaagt
     2581 aaatccgaga gaccggattt aggtagcgct aaaatagttg tttctggcgg taggggacta
     2641 caaagtgcag aaaaatttaa attaatcgaa gagttagctg atactttggg cgcagcagta
     2701 ggggcatcca gagctgcagt agatgccggt tttgttccta atgattatca ggttggtcaa
     2761 accgggaagg tagtagctcc tatgctttat attgccgtgg gtatttctgg agcagttcaa
     2821 catttggcag gtatgaagga ttccaaggtt attgttgcaa ttaataaaga cgcagatgca
     2881 cctatttttc aaatcgcgac ttatgggtta gtaggtgatc tgtttgaatt agttccacaa
     2941 ttaatagaac aattaaagaa tcgataagag gtaacaatat gttagtaggg gtaccaaaag
     3001 aaatcaaatc gcacgaaaac agagtaggac ttgttccctc gagtgttaga gaaattatta
     3061 gggcaggaag tgatgtattg gtagagaagg gagctgggct tggtatcggc atatcggatg
     3121 atgattacag aaatgccggg gctgaagtgg ttgatacggc tgatgatgta tttgccaatg
     3181 ccgaattaat agtcaaagta aaagagcccc agcctattga atgtaaacgg ttgcgtgaag
     3241 ggcaaacatt attcacttat ttacatttgg ctcctgatcc tcatcaagca cgtttattaa
     3301 aagaatcagg ggttacagcg atagcttatg aaactgtaac acaggatgat ggcgggttac
     3361 ctttacttac tcccatgtcc caagtggctg gtcgtatggc aatccaggcg ggtgcacatt
     3421 gcttggagat ggctcaaggg ggtagcggta tattactagg tggtgtacct ggtgttgctg
     3481 cagccaatgt cgtggtcatt ggcggtggag tcgtaggaac aaatgctgtt cgcatggcga
     3541 tggggatgga agctcgtgtt attgtgctag atcgttcact gcaacgttta aatgaattgg
     3601 attttcagtt tggttctaaa ctcaatacag tttattcaac tgcagattcc atagaaaaat
     3661 acgtatccag cgctgattta gtgattggtg ctgtattggt accaggtgct gcagccccca
     3721 aattagtgac tcgctctatg ctgagctcaa tgcgtcctgg ctccgtttta gtcgatgttg
     3781 ctattgatca aggagggtgt tttgagacga gtcgtgcaac aacccatcaa gaacctactt
     3841 acgtggtcga taacgtagtg cattattgtg tggccaatat gcctggtgct gtacccagaa
     3901 catcaacatt tgctttaaat aatgctactt tacctttcat tttaagtatt gttaccaaag
     3961 gggttaagct ggctttattg agtgacccgc atttacttaa tggcctgaat gttcaccagg
     4021 gtaaaatcac ttatgaggct gttgctcgtg atttaggata tgaatacacg ccagctgcag
     4081 ctgctttatc aggacgataa ttattagatt aaaacccgta ttacacaaat tgaatacggg
     4141 ttttgtgctt catcagaata taaatcttca aattaaaaca gactttcttc cactggtgct
     4201 agctcttctt cttgctgatc attatgtgca ttatagacag gaccttgatc ctcttcagat
     4261 ggaacctcat tttgtcgaaa atactcaatg atgccatttt cctggttagg cttagcaagc
     4321 aaaccactta ctggatctat tcgaaccgct ataacatttt caggctgctt caattcagtt
     4381 tcaggtttgt cttttaatgc tactttcata aaatcaatcc atagtggaag agcaagattt
     4441 gcagcatatt catgcaatga tttgggatta tcaaaaccaa tccaggttgt tactaccaaa
     4501 ttagggttaa atccggcaaa ccacgcatct acttgatcat tagtagtccc tgttttaccg
     4561 gcgatgtctt gacgatttaa tactcgtgca gcacgtccag tgccatgctg tattacatcg
     4621 cgcaatgccg tattcattaa aaaggcaatg tcttcagaaa taacgcgcgg tgccaaggta
     4681 gaagcatcaa ttttgtcttt atcacaaggg tcacatacaa cacttggttt ggcttgtaac
     4741 agaattttac catcagcatc tgttatatgg tcaatgaggt agggctctac tttataacca
     4801 ccattagcaa agactgcata agcagtggtt aaatccatag ggctaatgga caaacttcct
     4861 aaagcaagtg ataaggctct aggcaatgat tttttattaa agccaaagcg agttaagaaa
     4921 tctatcgtat aatcaatacc tatatcatca agaattctta tagataccaa atttttagat
     4981 tgaactaatg cctgtttcag gcgtgtaggg ccattgaatt ttagattcac attgtgtggc
     5041 cgccataaat tcggttggct gggatcgtct acaacgatgg gggcatcatt aattaaggta
     5101 gcaagattat aaccattgtt caaagccgca gcgtaaacaa atggtttgaa actggagcct
     5161 ggttgcctgc tggattgggt agctcggttg aatttacttt ttgagaagtt aaatcctccg
     5221 accaaaactt caatggcgcc attttttgga ttcatagcaa ccattgcaga ttctgcttca
     5281 ggaatttggg cgagttgcca ggatttttcc gtggggtgta cataaattat atcgcctgct
     5341 gctaccactt gttgtgcttt ggttggcgat ttcccaaccc agcctcctct tttcagtgct
     5401 ggcctggccc aggacatacc ctcccaggga ataataatta tttgcccgtt ctgtaatgcc
     5461 gcagtcgctt ctttttctct tacttcagtg accactgctg gaatcaggct attcagctct
     5521 ggatattgcc gcaagtattt ttgcctaatc ttgggagatt ggctgtcttt ttcacctata
     5581 ttggcaattg gccctctgta gccatgtcga tgatcataag caatcagatt tttttctacc
     5641 acttcattgg cagtattttg taatttgcca tcaatagttg tatatacctt atagccttta
     5701 gtgtacgctt cggggccaaa attgtcgtac agagactgac gaatcatttc agcaacataa
     5761 ggtgcttcca ccttgatatt agttccatgg tattttgcag taattggttg attgactgca
     5821 ttttggtatt gttcttcatt gatataatgt tcttccagta atcgctccag tacatggtca
     5881 cgacgttttt ttgcagcaag cgggttagcg ataggattct gagttgaggg agcttgagga
     5941 agtcctgcta tcatagccaa ttcagccaga ttaaggtctt taagggattt tccaaaataa
     6001 accatggcag ccgcgccaac accgtaagcc cgattaccca gataaaccct gtttaagtaa
     6061 agttccaaaa tcttttcttt acttaattcc cggtcaattt tgatagcaag catgatttca
     6121 ttgaatttcc gtaaaaatgt ttttttacgg cttaagaaaa aattacgagc tacttgcatg
     6181 gtgatcgtac tgccgccttg agatttggtt ccagttttga gcatgcttac tgcggctcgg
     6241 cccaaaccca taacgtctac gccagggtgc tcaaaaaatc gttgatcttc tgttgcaatt
     6301 aatgcatgga tcagagtagg gggaatttcg tcataagtta cgggaatacg ttttttttca
     6361 ccgtattcct gaattaataa accttcttta ctgaaaatct gtaatggtac ttgtaattgt
     6421 acagttttta atgaatcaac attaggcaat tggctttcga gataaaggta tagaaggctg
     6481 ccagccacta ttaggagaaa aaacaggctc atcagcgccc aaagaccttt acgccagaag
     6541 tatgccatct ttttcataga atttcgtttt cgttaaattt gaatagtgca atattataca
     6601 gtgaatctat ataatattgc gagatttata gggaaaaaac cggatttaaa tgctattttg
     6661 cgcctcacag ctatggatgg agataatcat tgatgaagcg ccaatagata ttaatcccca
     6721 ttgccattac ccatagaaaa aggttgatta ggctgggtat ctgttgtttc agaggattta
     6781 accattggta aatcggtcac atcgagcatt gtttgttgtt tggcttgagt gcccagcttg
     6841 gtattgaaaa acagagagct gtatcccaga ggcaggacag ggttgaaaac tggacgaggc
     6901 atgctgcgag ttagttcgct tactttctct ttcagttttt ctatttcttt gtctttctcc
     6961 tgaattgtag cttgttgttc taaaattatt tttttatctg ttttagtttg ttctgttttt
     7021 gttagcagtt cgttttcctg ttcttggctg ctttcggtca atattcgggc tttctccaat
     7081 agttcaggta ccaagagcgc attttgatgc tctaagcata aagattcagc aagcgcttta
     7141 atttcttcat cagaagagca ttctgctgta tttttagaat taatatgaat gttcaatgct
     7201 tctaaaactt cttctttgat caaatttccg gagttgcaaa attctccgcc aacgtatcca
     7261 tcatttttca cacctgaaag agaaatgctg gtttcagagt tctctgtagc agaagtatat
     7321 tttacttcat aagttttact tttaggggtg atcattaatt gtttgagatc aattaaaagc
     7381 tgagcgatct gctttttata ttcttccagc tttgatggtt caaatgagct tttcctatcc
     7441 agcatggatt ttagaaaaag aacttcattt aaaataaaat acaagaatgg ttttctgtca
     7501 gggtaacctt tggtgcaatt ttgtattaac tcttcaaggc gtcccttaca ttcaacgtct
     7561 ttatcctgaa taatttcttt agccaaagca agtgcttttc ttcttaagag aatgggattg
     7621 ctttcaacta ttaacttatt taatacttta ggctggctgt catgataccg aatgattaca
     7681 gaagtcaatg tatagagtag ctcttctaat ctagtcatat gtaaagctcc tttttataaa
     7741 gctaaaaatc taaatgtgga aatcccctac aacttggtat ttcactatat tgaatgtttt
     7801 gcataagtgc aagtgcctat taactggtac attatttgaa aaaaacatac aagtagaggg
     7861 gttgttttac agttttttga cagaatataa aatctgattt gattgtaata ctatgattga
     7921 tatcatgaaa tttgcttggg catcgacaga aggcagaaat agaaatgcat ggatcaattt
     7981 tcaggttgat tataaacgct taacccaaag taataagtaa agtgattcca atttatagtg
     8041 gcttacgcta acattaatta ttcaatagtt aggaaaagat ctaaaaatag gagtgctttt
     8101 gggctgcaag gaaatataca gggagattat ataagtgctc aaattgctta aacctaagca
     8161 tcgttcaatt cttggcattg acatcagttc ttccgcagtg aaaattctgg aaatatcagg
     8221 ttccggagag gacatttgtg ttgaaggata tggtcgtgaa gaaatacctg cctctgccct
     8281 agaaggaaat atgatcaagg atgtcgatgt agtagcgaat tgcatcaaaa aattggtaga
     8341 aagattgagg tttacatcta aaaatgtggc attggctgtg cctgattcag ctgttatcag
     8401 taaagtagtg caaattaatg aaggattgac tgatcttgaa atggaggagc ttgtcgttat
     8461 tgaggcggat aagtatatcc cttacccaat agatgaaata aacctggatt ttgaagtgtt
     8521 aggccattca gccaagagtt catcactgct tgatgtttta attgtggctt caagggctga
     8581 aaacgttaat acccgtgttg aagcagctca tagagcaggg ttggaaccta aaattgttga
     8641 tgttgaatct tatgcagtag agcgctcagt acaacaaatt gcgcaagaat taccagctgg
     8701 gggacaagac aaaattgtgg caatagtaga tattggagcc aattatactc acttgtttgt
     8761 tttgcacggt atgcgactaa tcttttcaag agaagaaaaa tttggtggta tgcaacttgt
     8821 tgaagctatt gcagaacact atcatatgaa tttggagcag gctattgccc taaaaaatca
     8881 gggtaaatta ccagaggact atgaatctgc tgtattagaa ccttttaagg acatgattct
     8941 tttgcagatc aaacgtactt tgcagttttt ttattcaacc agtcaacatg gttttgtaga
     9001 ccatatttta ttggcaggag gaatggccag gcaaccagga ttggcagcgc ttattcaaga
     9061 acagttagga gtgactacta cagtggcaaa cccgctatcc catatgtctt ttggtaaaaa
     9121 agtgaatttg gatgtgatta acaatgatgc gcctacacta atggtggctt gtgggttggc
     9181 tttgaggcat gtcgagtaaa aaatgactga aattaattta ttgccatggc gtgaacaaag
     9241 acgtgagcag gaaaagaaat tatttacaac catgctattg atagggatta tcattgctgc
     9301 agcaattgtt tttttaatga actcttatgt ctctcattta ataaataatc aaatgaaccg
     9361 aaaccagatt ttgcaaaaag aaattactgc tttagatgag cagatcaagg agattaaggc
     9421 actaaaacaa atcagggaag gtttgatttc gagaatgtcg attgtacaga atctgcagtc
     9481 aacaaggact ttaatggttc acttatttga tgaattaata aaaattactc ctccaggcgt
     9541 ttacataacc aagatgcaga gagaacacga tatagtaaca ctctgggggt attctgagtc
     9601 gaatacaagt atttctattt taatgagaaa tatagaggct aatgtctgga tacagaatcc
     9661 tgtcctaact gaaattaaaa aagatgttca aaaaaaacag tctccaaata atgaatttaa
     9721 attaagtttt gtgcttaaac ccaaatatta ggctgataaa aatcatggac acggtcaatt
     9781 taaatgaatt aactctggag aatgtcgggc agtggccaat gccggttaaa atcggagtca
     9841 ttttaggcat cagtatactg ataattggat taggatattg gcttattgtt aagccaaatt
     9901 ttgagcaatt ggaaatacag gaagcccagg aagttgcttt gaaatcagag tttgaaaaaa
     9961 agcaacatca ggcagcaaat ctacaagcct atcgtaatca gttgcagcta atgaatgagc
    10021 gtttcgggac catgctaaaa caactcccgg cgaaaaatga aatgcccggg cttttggaag
    10081 aaatatctaa aacggggatt gcatcgggtt tgaaatttga attgtttgca ccacaacctg
    10141 aagtagtgca tgatttttat gtggagttac ctataaaaat tacgattgtc ggatcctata
    10201 tgcaattggc aagattttta agtcgtgtag ctgaaatgaa cagaatagtg actttgcacg
    10261 aatttagtgt tgagggggtg tcctccgggg atggtaaaac ggtctctcag gatgaattgg
    10321 tgatggctat cactgcgaaa atatacagat atcgggcaca atgataatta aaaacaacca
    10381 gactcttata ttaggctttt tcggaaattc tctgcgtgtt tcactcacag cagcgacttt
    10441 ccgaaaaagt ctattgacgg ggttattggt actattactt agtgcctgct ctggtgatag
    10501 cagtgacctt gttaagtaca tcaacgaagt gaaatcaaga ccaggaagac ctatcgagcc
    10561 tattcccaaa ttcgcccctt tgccaatttt tagatttcca gaaaacgaca ataggcgaaa
    10621 tccattcaaa cctattgatc aaaaaaagag aaatgatatt tatgccccag ataaaaaaag
    10681 gcctaagcag cctttggaat cttttcctct ggatgcttta aaatttgtag gtactttaaa
    10741 aaaagacaat gaaatctggg ctctgattaa gcaacctgac ggtcagatta gccgtgtgag
    10801 ggttggagat tacatgggac aaaattatgg acgtattgtt ttgattaaaa atgatctcat
    10861 taaattggaa gaaactgtac agaaatcagg aacgtgggaa aaacagtcca ccacaattaa
    10921 tttggatact ggcaagcagg agtaaggcgt gagaaaaatt atcgcggttt tgatattaat
    10981 tagtgccagt ttgagtatgg catatcctaa agataattca ctcatttcag taaaagtatt
    11041 accattgcct gaaaacagag tgcgcattga ttttcaattt tccaaaccct taaagcagtt
    11101 gcccgcaagc tttattactc aaaagccccc acgtttggtg ttagatttta tcagcactga
    11161 tatgcaaatt ccttcagatc agaaaactaa aaccattcaa ttaggctcat tacatagcta
    11221 taacattgtt gctgttgggg atagagtaag agccatattg gagctggatc gttcagtgtc
    11281 ctattctggc agtacggcgg gttccgttta tagccttgtc ctgaatggta aaagcaatga
    11341 tttatttgaa aatagcaagg aagtattcat aacgaaccag gttgtgaacg ccaagcatga
    11401 aattaaaaga attgattttc gtggtataga aaagcaaggc ggtagagttt taattgatgt
    11461 ttctgatact ggtattccaa ttgatgtaac tcaagttggg aaagaaatag tcgttaattt
    11521 cctgaatacc aggatccctt tgaatttaat gaaacggtat gatgtttctg attttcacag
    11581 ccctgtgcag attattacta tgcaacagga aggaaaaaag gttcgtatga ccattctcag
    11641 caaaggggat tatgggcatt ttgtttatca ggtcaataaa caatttatgg tcgatgtctt
    11701 tcctttaact gcggaagaaa ttcgtcaggc gaaattgaag aaacaggtat ttaccggtaa
    11761 acggatttct ttgaatttcc agaatattag tattcgagct gtgcttcaac tgctggcaga
    11821 ttttaccgga atcaatatgg ttgtcagcga caaagtgcaa ggagatatta cgctaagatt
    11881 aaatgatatt ccctgggatc aggcgctgga tatcatttta accacacaag ggttagataa
    11941 gcgcagaaag ggcaacgtca tgttgataga taccaaggcc tccatggata aaatggagga
    12001 agaacaactc aaaagtcaac agacaattga aaaacttgag cctattcgtt ctgatttaat
    12061 acagattaat tatgcaaagg cagcagatat tgctgttttg atcaaggata agcaaaattc
    12121 cctattatca gacagaggaa aaataagtgt tgatgccaga acgaatacaa tctggattca
    12181 ggatactggc accaaaattg aggaagtaag ggaactgata aagcaattgg atatccctgt
    12241 taagcaagtg cttattgagg caagaattgt tgaagtaact aaagatttta gtcaagatat
    12301 tgggatacgc tggggggtat caagaccaac tcacttaagc ggtacattgg aaggtgctaa
    12361 cgagttagcg agaggaacag caccagctaa tgttgtcccc ttggaaagac gattaaatct
    12421 tgatttggca gctgctcctt taactggagc tacaccggcc tcggtaggta ttgctctagc
    12481 caagcttaat gataacattc tgcttgatct ggaattgtct gctttggaaa gtgaagggcg
    12541 tgctgaatta atatccagtc cccgcctgat tactacgaat caacaggcag cagtcatcga
    12601 gtctggtgag gagattccct atcaggaggc aacatcgagt ggagcgacgg ctgttgcatt
    12661 taaaaaagca gtgttgagtt tgaaagttac cccgcaaatt acgcctgacg gtaaaatttt
    12721 gatggattta cagataaatc aggatactcc ttctccacaa acattccttg gtgttccagc
    12781 aataataaca aaggagattc agacgaatgt tctggttaat aatggacaaa ccatagtact
    12841 tgggggtatt tataagcaag ataagaacaa ggtaatcaat agaattcctt tctttggtga
    12901 gttgcctgtt gtcggtattt tatttagtaa tagacaaatc acattgaaaa atgaagaatt
    12961 acttattttt attacaccaa gaataataac gaatgcccta tcgataacaa cgatagaagg
    13021 acgtgaaaaa gatgtttata aataat
//
LOCUS       DATNSN010000096        13008 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.096, whole genome shotgun sequence.
ACCESSION   DATNSN010000096 DATNSN010000000
VERSION     DATNSN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.096"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            18..1172
                     /locus_tag="VAH76_002101"
     CDS             18..1172
                     /locus_tag="VAH76_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386709.1"
                     /translation="MPTKINENDITELNTSEFFKRKKANPKIVSKNDPQIIQSNQILK
                     LLNSKTRRKEAISAFLYSFADIDRNAVLDFIKVLQTYPADTPDTEIAQVIYQWYCSIG
                     KENKIGFKMTDQEYQLNKAQNIASIITANFSPRRKEQLKRKALLDIGAGDCAMTSLVS
                     ELLGMEANAIDIQSNIDWGGENSSDKKSKNDYMTKIHHHYTYDGRDLLGTLKGKKFAV
                     VMYNHSLHHFPSFQDQIESLKQVSQILEPGGILFLSEHANCFDDDIFDLSHILLNLRY
                     SIDKNQILTPSEAGDAIAKFKSEYQSHYFSKNILNAIATQLGFNLVKQEIRSATDVAK
                     ATFFCFVKKEPKIELPCSLRFFDTDAANGLPHHIEKSDSQQKKYSAESFK"
     gene            1366..2847
                     /locus_tag="VAH76_002102"
     CDS             1366..2847
                     /locus_tag="VAH76_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386710.1"
                     /translation="MPHGVAALCLIQAFSTFSYAVQYSSLALFITKQLGIANLSANSI
                     VGLFLSFSYIFQLIGGVIGSRFISNRLLFVTTILIQNMGLFVLAQANPSSLNIGLSLF
                     MVGSGLNVTSSNSMLTQCFIPQDDRRESAFFLSYAGMNVGYCIGYIACGFFDYSNQYQ
                     YLFYASILTNSIALLIIIKNWAYLGDKNFPLPDLTKRNWYFIKNILAIAFITLLIPFM
                     RVCFEFANFCNGLIIITCLFMFLIIFILGLKQKSSSDRKKMMAYLILAISSVLFWMIY
                     LTGPMGVTLFVKNNVDKHLFSFELATQWIKNINPVVIILGAPIMAMLINKLKAKGFHV
                     SVPVQFSCSFVFLALSFLCLIFGIICSNSYGYISVYWIIGHHILQGMSELLLGPVGYA
                     LIGRIAPPKLQGILMGTWMLVTGVAASFSQYFSNAMVQSESVDPLITNQDFLRAFKQL
                     GLWSLLCAVLLYFVSGKITKIINGVQDNKPVNQSKAIPIPYSN"
     gene            complement(2852..3433)
                     /locus_tag="VAH76_002103"
     CDS             complement(2852..3433)
                     /locus_tag="VAH76_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386711.1"
                     /translation="MLTIDREKFEEIKKQLKEKHLVCIQIAQWKKTPLWLRKITHQPE
                     IPPNFLNELSQIRDAIVSFDIDKNTLGLAVFYNELYKLLAPSYNVLTPLDTDLMNSHV
                     LLLQRISHYAGEVDGYIKSLSSTEVKHLDYTEFWSLLNEICSIQEVDRRKQAKEKFSQ
                     SIFFRLYQYNPKIYLDVAKSLEEPDEQSNTLDF"
     gene            complement(3650..4186)
                     /locus_tag="VAH76_002104"
     CDS             complement(3650..4186)
                     /locus_tag="VAH76_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1386712.1"
                     /translation="MSAKQKDLERLLELKKKQEDLQVLNEKDMQERIKLERKYMDFLQ
                     MTSQQMEEELKKRGPVKEVDVKGKDIDPIIEDYKKLYSKESWYKEPETKDGKTHLTFP
                     SQEAAGNFFKDQAGKNRSFIVIDGATNKVLAYSNGDGKLYNGNGSVYQGGDFKASKED
                     FTSFKMPEREDPKMGMQL"
     gene            complement(4319..5749)
                     /locus_tag="VAH76_002105"
                     /pseudo
     CDS             complement(4319..5749)
                     /locus_tag="VAH76_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(5783..6226)
                     /locus_tag="VAH76_002106"
     CDS             complement(5783..6226)
                     /locus_tag="VAH76_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEO1386713.1"
                     /translation="MSKRQNPLPLNKYWEHFTHEADIGVRGIGPTLTDAFEMGALALT
                     NVVADATLIKPSLSIKIHCEAPNQEILFTDWLNAVVYNMQIHNMLFCEFDVKLNRFAL
                     DAVIKGEKVDQKKHQPVVEVKGATYTELKVYKNNDIWIAQCVVDV"
     gene            complement(6196..6480)
                     /locus_tag="VAH76_002107"
     CDS             complement(6196..6480)
                     /locus_tag="VAH76_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEO1386714.1"
                     /translation="MGKFWSQRVTNTSNALDLEKGVFTWNDPQKIAASLLDSALNSNR
                     RKGTPYQSAMSMLNFYINRAGSKLAAGQKEILMEAKKELRLLAKDKTRYH"
     gene            complement(6564..8117)
                     /locus_tag="VAH76_002108"
     CDS             complement(6564..8117)
                     /locus_tag="VAH76_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HEO1386715.1"
                     /translation="MRIFSRFSDRLVIFAFLVIIFVPGIGIFFEKQADEVRSLLNREP
                     HQLPPINIKKIGRTDFKGIENWFVDHTLFMTSLSKFWSHVVYQLGASIKPGQAILGKE
                     DWLFLGNDYAASIDQYTGRNKPTEEEILLKLSVLKQMNHLAKQNNIPFLVVIAPDKHE
                     IYPEYLPANVHKSSNKNRLDLLQEGMLARGIDFINLRQKEIEAKSTLGKQYGDLYLKG
                     DSHWNYVGAYVAYQAISDYMQQKGLQSRQLQFHFIPRETTYSDLTNFLQLTHIKSNNP
                     LPDVSNLKIDLFGRDINGKEIKLDDFQGNPNGVILIAPYENINKAVQNKQTCLLIGDS
                     FSESLSFYFHNDFYNTVRIHSGNTSWNLSDLIQKYHPDLIVYEKVERDLLYPLVNFQT
                     TANQMSLELPKQALAARGELDKFKIGPDTISVNGWAYIPDLDAGNGEVFLKLSMGTHT
                     YLYSMNKMQKQSVNLAFKQDGKHLDLSGFNGTISRKDLPSGLYKVSLIVLNDEVVGET
                     ELPGTYTLS"
     gene            complement(8120..9541)
                     /locus_tag="VAH76_002109"
     CDS             complement(8120..9541)
                     /locus_tag="VAH76_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEO1386716.1"
                     /translation="MVFSSLIFLFLFLPCLNLTYLVCFKSIKWQNFVLMLFSLSFYYW
                     GEKDHIFIMLACILINYLCGLLIEDSKKPGQRKIYLITSVSASLAMLMYFKYFNFFLD
                     SLNSVFDLNVMGISKIVLPLGISFYTFHALSYVVDVYWKKVPAERNFVTFVCYVSFFP
                     QLVAGPIVRYADIRHSFYRRYITRAGVRNGILRFCFGLAKKVLIANEVAKLADYVFYL
                     PANELTFWVAWLGAIAYALQIYFDFSGYSDMAIGLGLMFGFKYKENFNFPYNALSIQE
                     FWRKWHISLSSWFRDYLYIPLGGSYKSRFRTQINLIIVFGLCGLWHGANYTFIVWGLF
                     HGFFLILERTHFGYFMKKLPKVIRHGYVLSVVTVGWVFFREDNIQSALVFLKTMFIPA
                     EFNLDSIAACINIILIFAFIVGIILALGFNSRIRDSLILSRNKNWIIAYKQSSKVLGI
                     LLLLVSILLLSTNSYNPFLYFRF"
     gene            9739..10377
                     /locus_tag="VAH76_002110"
     CDS             9739..10377
                     /locus_tag="VAH76_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386717.1"
                     /translation="MQDSFFSFLSSPINLLFLFVAFFLLFVTPWLWSMVNSTHNIHKL
                     LDLIGIKKKENSRVVNGLLLLPLLLITIFIEWLWPLENITLFPILASPYIKFNIIAVI
                     SVVILLILIQFDKKKSMELWLLAYAAVLYLLYLIIIMATVVVGMNAFPASHYIFAPES
                     SMTTNKTIIYCLLIAHLIFVWLTLSLKFIWRLLLMILYTLALSFAYFSFIVS"
     gene            10610..11710
                     /gene="wipC"
                     /locus_tag="VAH76_002111"
     CDS             10610..11710
                     /gene="wipC"
                     /locus_tag="VAH76_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipC"
                     /protein_id="HEO1386718.1"
                     /translation="MNRMALFIIFIIHCFFSQSFAEQEKPYNELYVKQANLKQYPREI
                     NSYPPGVEITIGDLHGNALKLLYFLIRNDVIKIDKEDYKLFVTIYQKNPNELTTKDLS
                     FFQIIVNSAEINTQHKIRFLGDDLCDRGMNDYYTLVIYKKLDQANVPFEVILSNHGNF
                     FLTAYERPEQSFNYNPYGEGENESTVQSMLNMGRLIDRGLIDKQDILEMIQYHYLKHI
                     VLPGYTHNKDKNELTIYTHAPIDLGIISALANDLQVPFKDSNLYELTKSLDAINSKIK
                     QWILSNTFTRHYKELNEAHNQTNTPSPIKQILWNRDYSILNRHANPNNKPYGINYVHG
                     HDSMPNVFDLDNLFGKGEDFYQGPYAVHITHS"
     gene            complement(11758..12981)
                     /locus_tag="VAH76_002112"
     CDS             complement(11758..12981)
                     /locus_tag="VAH76_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEO1386719.1"
                     /translation="MIIYLLWLLAVTSFNFSTSTITVSKAEPQGNFLYSEIPSIKMPL
                     NEIKTLLQKEGNSLQPAVIDKVITTIQCANAYQVDRNNILTIIDYSMPSNQKRLWVFD
                     LDKKELLFHTYVSHGIKSGTLLTDKFSNKFDSKASSIGVYKTEQVYYGREGLSLRLVG
                     LDTKFNDNAFNRYIVMHGGWYMDEQFIKRYGRPGRSWGCPALPLPIKKQIIDTIKDNS
                     LLVVYYPSDEWFNKSKFLNCSKQKSDQVAINRLSETQAPVDDEIREDILFVDLNKNNS
                     REEHEPIITMSADAYERIFHSQPPLSRMLRRQINNAEYIALSKEEFNKLVLQGNREGL
                     GEIHFVIPVIIMEHGYYETQMQIVNMGKIKEVQPNSDTSRITQEPAKSYRIDFESKPA
                     LNLKTTNRFIRWLGL"
BASE COUNT         4095 a         2605 c         2164 g         4144 t
ORIGIN      
        1 ttaactataa gagtgatatg ccaaccaaaa ttaatgagaa tgacattact gaattgaata
       61 catctgaatt ttttaagaga aaaaaagcaa accccaagat cgtttcaaaa aatgatccac
      121 aaataataca atccaaccaa atactcaaac tattaaactc caagactcgc aggaaagagg
      181 caataagcgc ttttttatat tcatttgctg acatagacag gaacgccgtt ttagacttca
      241 ttaaggtttt acaaacctat cctgcagata ctccagatac tgaaattgca caagtgatct
      301 atcaatggta ttgctcaatt ggtaaagaaa ataaaatcgg gtttaaaatg actgatcagg
      361 aatatcagct gaataaagct caaaatattg cgagtattat caccgccaat ttctcaccgc
      421 gcagaaaaga acagctcaaa agaaaagcgc ttctggatat aggggctggt gattgtgcca
      481 tgactagttt agtaagtgaa ttattaggga tggaggcaaa cgccattgac atccaatcaa
      541 acattgattg gggtggtgaa aatagcagcg ataaaaaaag taaaaatgat tacatgacaa
      601 aaattcatca tcattacacc tatgatggtc gagatttatt aggtacctta aaggggaaaa
      661 aatttgcagt ggttatgtat aatcattcat tacatcactt cccttctttt caagatcaaa
      721 tcgagagttt gaaacaggtc agtcaaattt tagaaccggg aggaattctt tttttatcag
      781 aacatgcgaa ttgttttgat gatgatattt ttgatctatc ccatatttta ttaaacttaa
      841 ggtatagcat tgataaaaat cagattctta caccgtcgga agctggtgat gcaattgcaa
      901 aatttaaatc cgaataccaa tcccattact tttcgaaaaa tattcttaat gcaatcgcca
      961 cacaacttgg gtttaatctg gttaaacagg aaattcgttc tgcaacagat gttgctaaag
     1021 caacattttt ttgctttgtt aaaaaagaac ctaaaataga attaccctgt tccctgaggt
     1081 tttttgatac cgatgcagcg aatggattgc ctcatcatat agagaaatct gactctcaac
     1141 agaaaaaata ttctgctgaa tcatttaaat aaaataacct atacaattca aacaggtttg
     1201 attaacgaag cttaatcaat atttctttcc taattttact aaagtaaagt ttggaaagaa
     1261 attgaatggc tggttgttac attcttgaag gcaaatgatt attgatggta aagtctattc
     1321 tcataaaatc agccaaaatg tttgatgatt aaaataattc gggaaatgcc ccatggagtt
     1381 gctgcgctct gcttaattca ggcattttcg actttttcat acgctgttca atattcttcg
     1441 ctcgcacttt ttattaccaa acaacttgga attgccaatt tatcagccaa tagcatagtg
     1501 ggcctgtttt taagttttag ctatattttt caactaattg gcggcgtcat cggaagccgt
     1561 tttataagta acagactact atttgttaca actattctta ttcaaaatat gggtttattc
     1621 gttttagcgc aagcaaatcc gtcctcactt aacataggct taagtttgtt tatggtaggt
     1681 tcgggtttga atgttacttc cagcaacagc atgctgactc aatgtttcat accacaggat
     1741 gatcgcaggg aaagtgcatt ttttctcagt tatgccggca tgaatgtcgg ttattgcata
     1801 ggttatatag cctgtggttt ttttgattac tccaaccagt atcaatattt gttctatgca
     1861 agcatcctga ctaattccat tgctttattg attattatca aaaactgggc ctaccttggc
     1921 gataaaaatt ttccattacc tgatctcact aaacgaaact ggtatttcat aaaaaatatt
     1981 ctggcaatag cattcattac actccttatt ccttttatgc gggtatgttt tgagtttgca
     2041 aacttttgta atggattgat aataattacc tgtttattca tgtttcttat catctttatc
     2101 ctggggctaa aacaaaaatc ctcctccgac aggaaaaaaa tgatggcata tttaattttg
     2161 gcaatcagtt cggttttatt ttggatgatt tatctaactg gcccgatggg tgtaactctt
     2221 tttgtcaaaa ataatgtgga taagcatctt ttcagctttg aattggctac tcaatggata
     2281 aaaaatatta atcccgtggt cattatttta ggagctccaa tcatggccat gctcattaac
     2341 aaattaaaag caaaaggttt tcatgtctct gttcctgtgc aattttcctg ttccttcgtg
     2401 tttttagccc tgtccttttt atgtcttatc tttggaatta tttgttcaaa ttcctatggg
     2461 tatatcagcg tatactggat cataggccat catatcttgc aaggaatgtc tgagttgctt
     2521 cttggtcctg ttggctatgc gttaattgga agaatagctc ccccaaaact acaagggatt
     2581 ttaatgggca cctggatgtt ggttacaggt gtcgcagcct ctttttccca atatttctcc
     2641 aatgccatgg tgcaatcaga atctgtagac cccctaatta caaatcagga ttttctaagg
     2701 gcatttaagc aattgggact ttggtcttta ctctgcgctg tcttgttata ttttgtttca
     2761 ggtaaaataa ccaaaataat caatggggta caagacaata aacctgtcaa tcagagcaag
     2821 gctatcccaa taccttactc caactaatca tttaaaaatc cagagtattg ctttgctcat
     2881 cgggttcttc cagactttta gcaacatcca ggtaaatttt cggattgtac tggtacaaac
     2941 ggaagaatat gctttgggaa aatttttcct ttgcctgctt gcgcctgtct acctcttgaa
     3001 tagaacatat ctcattaagc agtgaccaaa actcggtgta atccaaatgc tttacttcag
     3061 ttgatgataa actttttata tatccatcta cttcacccgc atagtggcta attctctgaa
     3121 gcaatagcac atgactattc ataagatctg tatcaagtgg tgttaataca ttgtacgatg
     3181 gagctaaaag cttatacaat tcattataaa atacagccaa acccaaggta ttcttatcaa
     3241 tatcaaagga tacaatagca tcacgaatct gacttaattc gtttaaaaaa ttggggggaa
     3301 tctctggctg atgagtgatt tttctcaacc acagcggagt ttttttccat tgcgcaattt
     3361 gaatgcaaac cagatgtttc tcttttaact gctttttaat ttcctcaaat ttttctctat
     3421 caatagttag catgtgtttt ctcttaaaaa aggcaagcta taccctggaa gttagggtat
     3481 agtttccaaa gttgggaata tttgatacca cttaagtggt tttaggccaa atgatacatg
     3541 gcctgataat ttgtgtcaaa aaaaaatcaa agtggccttt atttgaaaca agagaataaa
     3601 ccatcataac atgattatct gcatcgaatc cgatttaaac caaagcagat taaagttgca
     3661 tccccatttt aggatcttct ctttcaggca ttttaaagct ggtgaaatct tcttttgacg
     3721 ctttgaagtc accgccttgg taaacactac cattcccatt gtatagtttt ccatcgccat
     3781 tagagtaagc tagcacttta ttggttgccc catcgataac aataaaagat ctgttttttc
     3841 cagcctgatc tttaaagaag ttgcctgctg cttcctgaga cgggaaagtc aaatgagttt
     3901 ttccgtcttt cgtttcgggt tctttatacc aactttcctt actataaagc tttttgtagt
     3961 cttcaataat agggtcaata tctttaccct tcacatcaac ttcttttaca ggacctcttt
     4021 tcttcaattc ttcttccatt tgctggctgg tcatctgcaa aaaatccata tattttctct
     4081 caagctttat tctttcttgc atatctttct cattcaaaac ttgcaagtct tcctgttttt
     4141 tctttaattc aagtaatctc tcaagatctt tttgttttgc gctcattctg gatatctcct
     4201 cgtacttatt gagctaattt tagtctaaaa atttatcgaa aattgctctt agtttatttt
     4261 atttacagat tccttacaat tataagctgc tccattcacc tcaataaaca atggattgtt
     4321 aacctttgac acaaatgatg ggaattaatt tagcaacttt ttttgccaaa cccgacattt
     4381 cagcggcatc aacaatagaa tgaacatctt tataagctcc tggagcttcc tcagcaaccc
     4441 cacggtatga tccactacga attaaaatcc cttgttgagc caatttatct actatgtctt
     4501 tcccgttcca tttcttcatc gcctgatgac gactcatagc gcgacctgct ccatgacatg
     4561 ccgagctaaa agatttatgc tcagaacctt tgactcctgc taatacatag gagcctgttc
     4621 ccatactccc tccaataatc acaggttgac ctatatgaga aacagcttta ggtagttgtg
     4681 ggtgacctgg acccaaggca cgagttgctc cttttcgatg aacaaataag cgctttttct
     4741 tgccatctat ctcatgaaat tcttctttac aagtattgtg agaaacatca tagattagat
     4801 caatctgagt gcgaggtaaa atatcctgaa acacattgcg cataaaatga gtgatgattt
     4861 ctctgttggc taaagcgcaa tttattcctg ctctcatagc gcccaggtaa ttttccccta
     4921 gctttgaatg aataggagca caagccaact ccctatccgc cagcttgata ccataatgtt
     4981 gtgattcaat aaccatggaa cggaggtaat ctgtaccaat ttgatgccct aatcctctgg
     5041 aaccacaatg gatactcact acaatatccc cttcatgcaa atttaaagct gttgctatat
     5101 cggaacagta gatttgcctg acttcctgca cctccagcta atgattacct gagcccaagg
     5161 tacccatttc atttttctgg cgctttttag cttgctcaga gacaaaatca ggaatagctc
     5221 cttccacaca accgcgatcc tcgatacgct ctaaatcagc gacttcgcca tagccttgtt
     5281 ttactgccca ctgtgcgcct cctcgcatca tatcatcgag ctgtttgata gttaaatgaa
     5341 tgccgcttct actacctact ccagctggaa tatgagtaaa taaagcgtct gcaaggctaa
     5401 ctttaacaga ctccaattca tctcgcttca atccagtaga taagagcctc actccgcatg
     5461 aaatgtcaaa gcccacacca cctgctgaaa taacccctcc atcatccgga tcaaaagcag
     5521 cgacaccgcc aatcggaaaa ccatatcccc aatgcgcatc aggcatggca tatgcagcag
     5581 aaacaattcc cggcaaagta gcaacattag ataattgttc gtaaactttc atatccatat
     5641 caagaatcag ctgttctgaa gcgaaaataa ctcctggcac caacatcttt ccatgttgcg
     5701 gtatttgcca ttcaaaatca tttatttttt tcaagagact taaatccatc actgttcacc
     5761 acattaattc ctgctaagga gtttatacat caacgacaca ttgagctatc caaatgtcat
     5821 tatttttgta tacttttaat tcagtatagg tagccccttt gacctcgaca actggttgat
     5881 gctttttttg atcaaccttt tcacccttaa tgacagcatc caaagcgaag cgattgagtt
     5941 ttacatcaaa ctcacaaaat agcatattat gaatttgcat attataaacc acggcattta
     6001 gccaatcggt aaataaaatt tcttgattgg gtgcttcaca atgtattttt attgataaag
     6061 aaggcttgat tagggttgca tctgcaacta cattcgttaa tgccaaagcc cccatttcaa
     6121 aggcatctgt gagtgtaggt ccgatgccgc gaactccaat atctgcctcg tgagtgaaat
     6181 gctcccagta tttatttaat ggtaacgggt tttgtctttt gctaataatc gcaactcctt
     6241 tttagcttcc attaaaattt ctttttgccc ggcagctaat ttgcttccag ctcgattaat
     6301 gtaaaaattc aacatagaca tcgctgattg ataaggtgta cctttgcgcc tattgctgtt
     6361 taatgccgag tcaagcaaag aagcagcaat tttttggggg tcattccagg taaatacccc
     6421 tttttcgaga tcaagcgcat tgcttgtatt cgtcactctt tgtgaccaaa attttcccat
     6481 tgattcgacc aactgaatct catacactta tattatagcg atttaatata agctctgctg
     6541 ttttgaatca gggccaatac catctaactt aaagtataag tgcccggaag ctcagtttcg
     6601 cccaccacct cgtcgtttaa gacaatgagt gatactttat atagaccaga aggcaaatcc
     6661 tttcgcgaga tagttccgtt aaaccctgac aaatcaagat gcttaccatc ctgtttgaaa
     6721 gccagattaa cactttgttt ttgcatttta ttcatactgt ataaataagt atgagttccc
     6781 atcgacaact ttaaaaacac ttccccattt ccagcatcta aatcgggaat ataagcccag
     6841 ccattgactg atattgtatc tgggccaatc ttgaatttat cgagctctcc cctggcagct
     6901 aaagcctgtt taggaagctc aaggctcatt tgatttgctg tagtttgaaa attaacgaga
     6961 ggatagagca aatcacgctc gactttttca tacacaatca aatcaggatg gtatttttga
     7021 attaaatcag acaaattcca ggaggtattc ccggagtgaa tacgtactgt attataaaaa
     7081 tcattatgaa aataaaaact gagagactcc gaaaagctgt ctcctattaa taaacaggtt
     7141 tgtttgtttt gtacagcttt attaatattc tcgtaaggcg caatgaggat tacaccattt
     7201 ggattgccct ggaaatcatc caatttaatc tcttttccat taatatctct gccaaacaaa
     7261 tcgattttta aatttgaaac atcgggaaga ggattattag atttaatatg tgtcaactgc
     7321 agaaaattgg taagatcaga gtaggtggtt tctctgggta tgaaatgaaa ctgtaattgt
     7381 ctgctctgta accctttttg ctgcatgtaa tctgatatcg cctggtaagc aacataagca
     7441 ccaacataat tccagtgact atcccccttt aaataaagat ctccatactg tttgccaaga
     7501 gtacttttgg cttcaatttc cttttgtctc aaattgataa aatcaatacc tcgagcaagc
     7561 attccctctt gtaaaaggtc aagcctgttc ttattggagc tcttatgaac gttagccggt
     7621 aaatattccg gataaatctc atgtttatct ggggcaatga ccaccagaaa aggaatgtta
     7681 ttttgcttag ccaagtgatt catctgtttt aagacagata atttcaagag aatttcttcc
     7741 tcggtaggct tatttctccc tgtgtactga tcgatggatg ccgcgtaatc attgcccaaa
     7801 aacagccaat cctctttccc taaaatggct tgcccaggtt tgatgctggc tcccaactga
     7861 tatacgacat gactccagaa tttactcaac gaggtcatga acaaagtatg atcaacaaac
     7921 cagttttcta tgcccttgaa atcagtacgg cctatttttt ttatattaat gggggggagt
     7981 tgatgaggtt ctctgtttaa tagagatctc acttcatccg cttgtttttc aaaaaaaata
     8041 ccaatccctg gtacaaaaat gattaccaga aaagcaaaga tgaccagcct gtcagaaaac
     8101 ctggaaaaaa tacgcatcat caaaacctaa aatagaggaa agggttataa gaattagtgc
     8161 ttaataataa tattgaaacc agtaaaagca gtattcccaa aaccttgctg gattgcttgt
     8221 atgcgataat ccagttttta tttctactta agatgagact atcccttatt ctgctgttaa
     8281 accctaacgc aagtataatg cccacaataa aagcaaaaat aagaataata ttaatacatg
     8341 ctgctatgga atcaagattg aactcagcag gaataaacat cgttttaagg aataccagtg
     8401 ccgactgaat attatcttct ctgaaaaata cccatccgac tgttacaaca gaaagaacat
     8461 agccatggcg aataacttta ggtaattttt tcataaaata gccaaagtgg gttctttcca
     8521 gaataaggaa aaacccatga aataaccccc aaacaataaa ggtataatta gcgccatgcc
     8581 ataaaccaca caaaccaaat acaatgatca agttaatctg cgttcgaaat cttgacttat
     8641 aactccctcc gagcggtata tataaataat ctcgaaacca gcttgataat gagatatgcc
     8701 acttccgcca gaactcctgt atcgataagg cattataggg aaaattaaaa ttttccttgt
     8761 atttaaaccc aaacatcaaa ccaaggccta ttgccatatc agaatagccg gaaaaatcaa
     8821 agtagatttg cagagcatag gctatagccc cgagccaggc cacccaaaaa gtcaactcgt
     8881 tggcaggcaa ataaaataca taatcagcaa gctttgccac ttcattagcg ataaggactt
     8941 tttttgctaa accgaaacaa aaacgcagaa ttccattgcg tactcctgca cgagttatat
     9001 atctccgata gaatgaatgt cttatatctg catagcgaac gattggtcct gcgaccaact
     9061 gcgggaaaaa agatacataa caaacgaaag taacaaaatt acgttctgct ggtacttttt
     9121 tccaatacac atcaactaca taacttaaag catggaaagt ataaaaactt attccaagag
     9181 gcaacacaat tttactgatt cccatgacat ttaagtcaaa gacactgttc agtgagtcaa
     9241 gaaaaaaatt aaagtactta aaatacatga gcatggccag tgatgcgctc acggaagtga
     9301 tcaaatagat ttttcgttgt cctggttttt tactgtcctc aatcaatagg ccacataggt
     9361 aatttatcaa aatgcacgcc agcataataa agatatgatc tttctcaccc caataataaa
     9421 aggacaagct aaagagcatt aaaacaaaat tttgccactt gatactttta aaacagacta
     9481 gataagtcag gttaaggcac ggaagaaata aaaaaagaaa tattaaagat gaaaaaacca
     9541 ttctatctcc gtgaaggcat ttgcggaatt aattaaagac gttagattca tcgcgtgaag
     9601 cgcattatag ggaagtgtta tccgttgtgc aaatagaata cgataaaaag aaccatctgc
     9661 aaaatctgaa ttgaagctgc cattataaca ttcattagct atacttaacc taaggcagca
     9721 ttcgttgatg aattaataat gcaagattcc tttttctcat ttttatcaag ccctatcaat
     9781 ttactctttc tttttgttgc attttttttg ctttttgtaa cgccatggct ctggagcatg
     9841 gtgaattcaa ctcacaacat tcacaagctg cttgacctta tcgggattaa gaaaaaagaa
     9901 aactcacgag tagtgaatgg actgttactt cttccactgt tactgataac gattttcatt
     9961 gagtggctgt ggcctttgga aaatatcacg ttgtttccta tactggcctc accctatatt
    10021 aaattcaata ttattgcagt catttcagta gttattcttt tgattttaat acaatttgat
    10081 aaaaaaaaat caatggagct ttggttgctc gcctatgcag cggtacttta tttattgtat
    10141 ctgattatca tcatggcgac agtagtggta gggatgaatg cttttcctgc aagccattat
    10201 atttttgccc ctgaaagcag catgacaacc aataagacca taatttattg tctattaata
    10261 gcccatttga tttttgtctg gctaaccttg tcgcttaaat tcatatggcg tttattgttg
    10321 atgatactct atactctggc cctgtctttt gcttatttct cttttatagt ctcttgaata
    10381 agctgtttct tttattttat tgctgcgtcg caaacgtttc tgtggcgctc atttactgta
    10441 gtaaactccc ccaaaacgtt tgcaccttgc cctaaagcaa aataatcagg ttgggcaaga
    10501 ggtctaatat aaagccattg ccttcatgat aaaaccaagg tatacctagc ttaccagatt
    10561 acggtcatcg acacagaaca ataaaaaatt tttacaggat gtagcattca tgaaccgcat
    10621 ggcacttttc atcattttta ttattcattg ttttttttct caatcatttg ccgagcagga
    10681 aaaaccatac aacgaattgt acgtcaaaca agcaaacttg aaacagtatc ccagggaaat
    10741 caatagttat ccacctggtg tagaaatcac tattggagat ttgcatggaa atgcattgaa
    10801 attgctctat tttttaattc gcaatgatgt gattaaaatc gacaaagagg actataagtt
    10861 atttgtaact atctatcaga aaaatcctaa cgaattgaca acaaaagatt tgtctttttt
    10921 ccaaattata gtcaattccg ctgaaatcaa tacccaacac aagatacgtt ttctaggaga
    10981 tgatctatgt gatcgtggta tgaatgacta ttacactttg gttatttata aaaagctcga
    11041 ccaggccaat gtcccttttg aggtgattct atccaatcat ggcaactttt ttttaacagc
    11101 ctatgaaaga cctgagcaat cttttaacta taatccttac ggtgaaggtg aaaacgaatc
    11161 cacagtgcaa tccatgctaa atatgggaag actgattgat cggggattga ttgataaaca
    11221 ggatattttg gaaatgattc aatatcatta cttaaagcat atagtgcttc ccggttatac
    11281 gcataataaa gataaaaatg agcttaccat ttatactcat gcgccaatcg atttaggaat
    11341 aatctcagca ttagccaatg atctgcaagt tccttttaaa gactctaact tgtatgaatt
    11401 aaccaaaagt cttgacgcaa ttaactccaa aataaaacaa tggatactct ctaacacgtt
    11461 cactagacat tacaaggagc ttaacgaagc acacaatcag accaacacgc caagcccaat
    11521 taagcaaata ttgtggaatc gtgattattc tatcctcaat cgccacgcta atcccaataa
    11581 caaaccctat ggcattaatt atgttcatgg ccacgactct atgcccaatg tgtttgatct
    11641 ggataactta tttggaaaag gtgaggattt ttaccaaggg ccttacgctg tgcatattac
    11701 acacagctag ctgtgaataa actcaaccct gtcattaaac agggtaacaa attgaaatta
    11761 tagccctagc caccgaataa agcggtttgt tgtttttaaa ttgagggctg gttttgattc
    11821 aaaatctatt ctatagcttt tagcaggttc ctgtgtaata cgagacgtat cagaattggg
    11881 ttgaacttcc ttaattttac ccatattgac gatttgcatt tgagtttcat aatacccatg
    11941 ttccataata atgacaggta ttacaaaatg aatttctccc aaaccctccc tattcccctg
    12001 aagaaccagc ttgttaaatt cttccttact caaagcaatg tattctgcat tgttaatttg
    12061 gcgtcttaac atgcgggaca aaggaggttg agaatgaaaa atacgctcat aagcatccgc
    12121 tgacatagta atgataggtt catgctcttc acggctgtta tttttattga ggtcaacaaa
    12181 caaaatatct tcccgtattt catcgtcaac aggagcttgg gtctcagaaa gcctgttaat
    12241 agcaacttga tcagatttct gtttactgca atttaaaaat ttcgacttat taaaccactc
    12301 atcgctagga taataaacaa ccagtaaaga attatctttt atagtatcaa taatttgttt
    12361 tttaattggc aaaggtaacg caggacagcc ccaactacgg ccaggccgac catatctttt
    12421 gatgaattgt tcatccatat accatccgcc atgcataaca atatagcgat taaaggcatt
    12481 atcattaaac ttggtatcca aacccactaa tcgtaaagac aatccttctc gaccataata
    12541 gacttgctct gttttataaa caccaatact gctggcctta ctatcaaatt tatttgaaaa
    12601 tttatccgtt aaaagagtcc ctgattttat cccgtgagat acataagtat gaaataataa
    12661 ttctttctta tccaaatcaa acacccataa ccgtttttga ttagaaggca tggaataatc
    12721 aataatagtc aatatattat ttctatccac ttgataagca ttagcgcatt gtatggtcgt
    12781 tatcacttta tcaataacgg caggttgaag agaattaccc tctttttgta acaatgtttt
    12841 tatttcattc aaaggcatct tgattgaagg tatttcactg tataaaaaat tcccttgagg
    12901 ctccgctttt gacactgtaa tcgtagatgt tgaaaaatta aatgatgtta ctgctaataa
    12961 ccatagcaga taaataatca tagtttttcc ttgtgattga gttgatct
//
LOCUS       DATNSN010000097        12987 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.097, whole genome shotgun sequence.
ACCESSION   DATNSN010000097 DATNSN010000000
VERSION     DATNSN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.097"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..662)
                     /locus_tag="VAH76_002113"
     CDS             complement(<1..662)
                     /locus_tag="VAH76_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO1386720.1"
                     /translation="MYENLLVGCHEEWKEILMKALNTMDSDYLKQIQEDSHWLPGLNQ
                     LFSAFSLPLSQTQYILLGESPYPRKISANGYAFWDNSVGLLWSSTGLSKEVNRATSLR
                     NWIKTLLIARGDLHGNTSQEAIAKINKSCLVQTAEDFFKGMMKKGILLLNASLIYSKG
                     KVPYHARHWKPFMQNLFEQLAIRKPDVQLILFGKIAEKIPANKLLIGLVSEHPYNVSF
                     IT"
     gene            927..2609
                     /gene="legC3"
                     /locus_tag="VAH76_002114"
                     /gene_synonym="ppeA"
     CDS             927..2609
                     /gene="legC3"
                     /locus_tag="VAH76_002114"
                     /gene_synonym="ppeA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     LegC3/PpeA"
                     /protein_id="HEO1386721.1"
                     /translation="MIMFLANCNIEELVTEHIKQFLADEELSFSGLKDLILSKAPIPW
                     IHSSVTATLLKSRDSDKTEVKKNLEQQSYKAQLAEDKIQKEQDDAEALKDKKLKEVLT
                     RELNHIPTQISEQQTELRLLHYKLERLFESQAKVDVIQHPDSPMKKIKPSSSHSASIE
                     RLQRSINEREIKIQSLFEQEVNNKIKLNEIEKRASVRSQHHTKRVKRAQARIGYNSTG
                     EDILSTLSGKNQSILLRSIQKQHHALEKKCSDLIQEADQINYPLFLEELQKYLNKKKH
                     TLSSQEVDALKSVIKYIKQHLEFEHEVINTQSSLHIKKQSISSQIVKLRELQNKLKTL
                     KNNNPNLTAANEELVSRNLELTAMKEHHADLRHRLGTPALLLFGLTFLFSIPLILTIS
                     GVIPFFIAPALLYILVSAPPTILLLSTLGVGIAAIVFSFKMHSNESAIKSNLQTIETN
                     SNQMSRNSQNLKSLETLTIPTLDMQIKKDENLRDQLMLSLQKQQRQAAQAFQKAKEVE
                     CLSYATSSLLNSGNTQSSDPSLSTHSEESDEGLNDSNDSLQALEEETVNEIA"
     gene            2745..4385
                     /gene="ppeB"
                     /locus_tag="VAH76_002115"
     CDS             2745..4385
                     /gene="ppeB"
                     /locus_tag="VAH76_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PpeB"
                     /protein_id="HEO1386722.1"
                     /translation="MSLATYDVKQELVKLTQNFFSIDHQFSELKNLLTDKKTIPWIHN
                     QIVDQLSQIKKSDEIAMIARLEKSAYEQQMKEDDDEKNRDVEELRQDLLRRNDLTRQL
                     EILRVRKEQYERELLIRNTVPHVHTHPDTPVIHQHPSQPISAPLLNPNNHVHTHLEAT
                     LSSQDIELSVRKINRRISEINKELEILDSRNNAREIRRGDREKRLQARLNYVQKTAGV
                     VNTLSPDNQKKLLSNIEKEKKSLTQQHSSLLLKADQLNYSIFLQQFELSLQSMQRPFQ
                     EADALKAIVKRMKEHLNYKEKAASIQSRLDNTVLTIGENLRRLTRLNTQLSSLQLANP
                     DLTRRNERLEEQNRELLESYNSHIKTRNKLFFPTLVITGLSLLFSIPLILTLTGIIPY
                     AIAPAVLLTLVIAPPALLLLAGLGVGIATIIYAVKAYFNNSTIESNQETIESNRRQMG
                     ANQKEIYTLENQTIPNLKKELLENEEIKNRLTDELQYIENLAEQALKQANEVEPYVYS
                     AMPFFNPEVVVHQHPSVGNGIVTPSAPPIDPLYPAVRH"
     gene            complement(4472..5728)
                     /locus_tag="VAH76_002116"
     CDS             complement(4472..5728)
                     /locus_tag="VAH76_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEO1386723.1"
                     /translation="MFALVDCNNFYVSCERLFKPALANKPILVLSNNDGCVIARSNEV
                     KALGVQMGVPYFAVKALCKQHKVHVFSSNYTLYADMSRRVMSVLEDNWPAIEIYSIDE
                     AFLDLNTLPQSEHDEFCRKLQKIVLRHTGIPVSIGIGATKTLAKLANHIAKKELRTPV
                     FNIENQYHWLAKIVVGEVWGIGRQWGKKLVQQGIYTAHDLAGMDLQLIKKLFSVVLQR
                     TVLELRGVSCGSLQDFESRKSIISSKSFGSMQTEYSALAQAISSHCARAWEKLRHQKL
                     MACHLSIFIKTNQFRLDLPQYQQTIGFKLINPTDDLRHLIRCAKLCLRKIFRSGFHYQ
                     KVGVYLGDLIEKNCLQLDLFNQVSEKELNQTERLMSVLDEINTKYGRHTLRLAAEGHV
                     KPWAMRAQSRSPRYTTSWSELPVVII"
     gene            complement(5728..6252)
                     /gene="umuD"
                     /locus_tag="VAH76_002117"
     CDS             complement(5728..6252)
                     /gene="umuD"
                     /locus_tag="VAH76_002117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEO1386724.1"
                     /translation="MSHGGARAGAGRPRGQGKYGESTKSVRIPESRIQDVMKYLQGEK
                     EFPAIPLYTSSVRAGFPSPGDDYIESMLDLNEYLIKHPASTFFVRASGESMINAGIYS
                     GDILIVDRGIEATHGKIVIVALNGELTVKRLSRQHGQVKLLAENPDFPPIDITEEYDM
                     VIWGVVTHVIHTVL"
     gene            6418..7641
                     /locus_tag="VAH76_002118"
     CDS             6418..7641
                     /locus_tag="VAH76_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO1386725.1"
                     /translation="MVKLYKEIINLIDNHQATMVEQLHQFCEINSGTTNLTGLAIMAE
                     TLCSAFKPIADTTELITLPPLSVIDMTGNTVMQSCGDVLFISKRPHLKRRILLSGHMD
                     TVYSASNPFQKLSYLDANQINGPGVADMKGGLIVILHALAAFENTRFAEDMGWDVLIN
                     SDEEIGSPASGSLFNKLAHRYQAALVYEPAMTPNGTLAKNRKGSGKLTLVATGRAAHA
                     GRAFDEGRNAICYLAEAITAVHALNGQKNGVTINVGKIAGGEALNVVPDKAVAQLDIR
                     ISLPEDEIWVRNELNKIIKQLERPDYSLNVHGTFGRPVKRICAGTERLFHRIQKIGEA
                     LGLTIDWKDSGGCCDGNNLAHYGLPVLDTLGVRGGNIHSSDEYILLDSLSERAALSAL
                     LLIDLAQGGLEDLIK"
     gene            7638..8681
                     /locus_tag="VAH76_002119"
     CDS             7638..8681
                     /locus_tag="VAH76_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEO1386726.1"
                     /translation="MMLFRSARDSDLDAIHQLAEISGVGMTTLPKDKELLKKRLEWSC
                     ESYQRNVTNPGCEYYFFVLEEQKNNTIVGTSAIEARTGYEIPFYSYKLSKRTRVCHTL
                     NIRSDYEVLSLVNDNQGRTEICTLFLHPDYRKNNNGLLLSKGRFLFMAQYPHRFAPIV
                     IAEMRGISDDKGNSPFWDNVGANFFHMPFVEADRLTLATNKQFIADLMPRNPIYVKLL
                     SPEAQAVIGKPHQSTIPAMNILLKEGFRYNNYVDIFDAGPTIESPLLEIKTISLSRIM
                     VIKNISDEASSSNRYLLANTQMDFRATISHAVFNERENTCIISKETAKLLDVKQGDSL
                     RISPIRTEDTPPL"
     gene            8695..10182
                     /gene="astD"
                     /locus_tag="VAH76_002120"
     CDS             8695..10182
                     /gene="astD"
                     /locus_tag="VAH76_002120"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEO1386727.1"
                     /translation="MSKLQIIQSKGQYINGEWIKGNGLILESTNPASGTLLWQGNNAT
                     DEEIANACYVAHRALKSWANTSFEERARYTKAFVEQVEKNREQLARLISLETGKPLWE
                     SQTEVSSVIGKVNLSIQAYQERTWPKQTETAEANACLRFKPHGIVVVLGAFNFPAHLS
                     NGHIVPALLAGNTVLYKPSEHTPAVAELIIQCWHDSGLPPGVINCLQGNANCGNTLLS
                     QDIQGVYFTGSYATGLRIHQQFCNRPEVILALEMGGNNPLVIDEVKDIDAAVYHTILS
                     TMITAGQRCTCARRIIIPDSQTGDLFLERFAKACKLMRIGSFDSQPEPFIGPVISHVQ
                     ALKHLHAQKQLIEMGGEIILPMSLLLEYTGLVSPGIIDMTRAKNPPDEEIFAPFAQIY
                     RYNHFDEAIQLANQTRYGLSAGLLSDNKDHYQQFYQNIRAGLINWNRPTTGAASSLPF
                     GGVGCSGNHRPSAYFAADYCAYPVASMEQPLLTTPVQRLPGLVLE"
     gene            10188..11534
                     /gene="astB"
                     /locus_tag="VAH76_002121"
     CDS             10188..11534
                     /gene="astB"
                     /locus_tag="VAH76_002121"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEO1386728.1"
                     /translation="MNVYELNMDGLVGQTHHYAGLSSGNIASTNNALSISNPQAAARQ
                     GLEKMRQLYNMGLKQGLLPPHQRPNLNLLYQLGFKGTPSEQINKAYKTAPELLSACYS
                     ASSMWTANAATVSASVDTEDNKVHFTAANLISNLHRHQEADFSKKLLEFIFSNSDYFN
                     HHPLLPKSMGTSDEGAANHNRLCQSHAHSGINLFVYGKKVLGNHQFEQSPIKYPARQT
                     KEASEAIARNHLLNPERVIFACQNPLAIDQGVFHNDVISVANEHVFLVHEEAFYNQAY
                     VLDQLREKADFPLVIIQISKEQISVSEAVDTYLFNSQLITLPDQKNMILIAPAECQAN
                     LKVKTCIDGLVADPQNPINSVYYLDLKQSMRNGGGPACLRLRVPLNDYELKAMHQGIL
                     IDNDLLDILDKWVLKYYRTELKIPDLADPQLLYECLDALDELTQILKLGSIYPFQS"
     gene            11546..12271
                     /locus_tag="VAH76_002122"
     CDS             11546..12271
                     /locus_tag="VAH76_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386729.1"
                     /translation="MWKTKFGKCIYISPSGYKVYQNLLYRWLTLGSEAWQTVINRYIP
                     RRPGLHYLQILTLMARKFPNDCCLLGLGGAGIAHLLGAQKTIRSIVAVESSEEVIQIA
                     KKFFMTDLIPHFTITHQNARDFVRENKKTFQHMLLDLYDATHFPDECHSMDFFIHCKK
                     QLTDDGFLAVNLANSQEQWALVQLIKKQFPSNIIIPIKKCANIVVIASKNDNKDLFIK
                     QVRNTNEIKKITWVKDWGYVAEY"
     gene            complement(12349..12714)
                     /locus_tag="VAH76_002123"
     CDS             complement(12349..12714)
                     /locus_tag="VAH76_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEO1386730.1"
                     /translation="MKRNALIIMLVYLTSTPVFAKNLGKYTYEIACKNCHAPNLAKAI
                     KAPPAFDKKAWKLRFKQAKIEAKNNPSQFESAMDYLLYNVKVGKGLMHHGGLCKEADV
                     PNTDCSDEALIEAINYMRK"
BASE COUNT         4220 a         2635 c         2368 g         3764 t
ORIGIN      
        1 ttagtaataa agcttacatt atatgggtgt tctgaaacca gtcctatcaa taatttattt
       61 gcgggtattt tctcagcaat tttaccaaat aagattaatt gcacatcagg cttcctgata
      121 gccaattgct caaataaatt ttgcataaat ggcttccaat gacgcgcgtg atacggaacc
      181 ttacctttac tgtatatcag ggaagcattc aataataaaa ttcccttttt catcattcct
      241 ttaaaaaaat cttccgcagt ttgtaccaga catgatttat ttatcttggc gattgcttct
      301 tgcgaggtat ttccatgcaa atcaccccga gcgatcaata acgttttaat ccagttgcgt
      361 agtgaggttg ctcgattaac ttccttactt agccctgtgg agctccacaa taaacctaca
      421 gagttatccc aaaaagcata cccattagca gaaattttgc gtggatatgg ggactcgcct
      481 aaaagaatat attgtgtctg gctcaaaggc aggctaaaag cagaaaacag ttgatttagc
      541 ccaggtaacc aatggctatc ctcttgtatt tgttttaagt aatcactatc cattgtattc
      601 aatgctttca tcagaatctc tttccattcc tcatggcatc caaccaataa attctcatac
      661 atcttaattt ttacccttcc gtcatgataa ttaaattttc aaattgaggt attttgataa
      721 ttacctatgt ttttaaacca ggttttcagc cactttaaca ctattcttag accatgagaa
      781 attgtcttat tttaacatct ggaaataaac cccacgttgg ccaatagctt atctgttgat
      841 tgcagcgatt ttcttgccat atcaatcgtg gtgtgttgcc tgaatattca aattacacta
      901 acatcattta aaaatgataa tctggagtga ttatgttttt ggccaactgc aatatcgagg
      961 aattagttac agaacatata aaacaatttc tagcggatga agagttgtct ttctctggct
     1021 taaaagattt aattctgagt aaagccccaa ttccctggat tcactcctct gtaacagcca
     1081 ctctgcttaa atccagagac tccgataaaa cagaagtaaa aaaaaacctt gaacaacaat
     1141 cttataaagc tcaactggct gaagacaaga ttcaaaaaga gcaggatgat gctgaagcat
     1201 taaaagataa aaaattaaag gaagttttaa ctcgtgaatt aaaccacatc ccaactcaaa
     1261 tcagtgaaca gcaaacggaa ctcagattac ttcattataa actagagcga ctctttgaaa
     1321 gccaagctaa agtcgatgtg attcaacatc ctgattctcc tatgaaaaag ataaagccgt
     1381 cctccagtca ctctgcgtcg attgagcgcc tgcaaagatc tattaatgag cgtgagatta
     1441 aaattcaatc actttttgaa caggaagtta acaacaagat aaaattaaac gaaattgaaa
     1501 agcgtgccag tgtacgctca caacatcaca ctaaaagggt taaacgcgct caggcaagaa
     1561 ttggatacaa tagtactgga gaggatattc tcagtacatt atcagggaaa aatcaatcca
     1621 ttttgttgcg cagtattcaa aaacaacatc atgctcttga aaaaaaatgc agtgatctca
     1681 ttcaggaagc tgatcagatt aattacccat tatttcttga ggaacttcaa aagtatttaa
     1741 ataaaaaaaa gcatacttta tcttctcaag aggtcgatgc attaaagtct gttataaaat
     1801 atataaagca acacttggaa tttgagcatg aggtaatcaa tacacaatcc agtctccata
     1861 ttaaaaagca atccatcagt tcccagattg taaaattacg agaattacaa aataaactaa
     1921 aaaccttaaa aaacaataac cccaatttga cagcggcaaa tgaagaatta gtttcacgta
     1981 atttggaatt aacggccatg aaagagcatc atgctgacct acgtcatcgc ttgggcacac
     2041 ccgcattact tttatttgga ttaacttttc tattttcaat tcctcttatt ttaaccataa
     2101 gcggagtaat tccttttttc attgcgcctg cgttgcttta tattttggtc tcagcgcctc
     2161 caaccatctt gctgctatca actcttggtg ttggcatagc agctatcgtt ttcagcttca
     2221 aaatgcattc caatgaatca gctattaaat ccaatttgca aacgattgaa acaaactcta
     2281 atcaaatgag ccgaaattcc caaaatttaa aatcattgga aacgcttacc atccccactc
     2341 tggatatgca aatcaaaaaa gatgaaaatt taagagatca attaatgctt tctttacaaa
     2401 aacagcaacg tcaagctgcc caggcattcc agaaagcaaa agaggtagag tgcctatcct
     2461 atgctacctc ttcactttta aattccggca atacccaatc cagtgatcca tcattatcca
     2521 cgcattcaga agagagcgac gaagggctaa atgactcgaa tgactctctg caagcgctgg
     2581 aagaagaaac agttaatgag atagcgtaaa ttgcctttct gtcaataaaa tttcaatgga
     2641 gcaagactga gtattaaccc cctgtaataa aactgctagt cccaatattg ataaattagt
     2701 ctttatgatc taaaatgcaa caacttgatt attgggagta atctatgtca cttgctactt
     2761 atgatgtaaa acaggaactc gtcaaactta cccagaattt cttctctatt gatcatcaat
     2821 tttctgaatt aaaaaactta ttaaccgata aaaaaaccat tccatggatt cataatcaaa
     2881 tagttgatca gttatctcaa attaaaaaat cagacgaaat agctatgatt gcgcggttag
     2941 aaaaaagcgc ttacgaacaa caaatgaaag aagatgacga tgaaaaaaat cgggatgttg
     3001 aagagttacg ccaagatctt ctccgaagga atgatttgac aagacaactc gaaatcttgc
     3061 gagtccgcaa agagcaatat gaaagagagt tgctcatccg caatacagta ccccatgtcc
     3121 atactcatcc ggacactccg gtaattcatc aacatccttc tcaaccaata tctgcaccct
     3181 tgctaaaccc taataatcat gtacacactc atcttgaagc cactttaagt agccaggata
     3241 ttgagctgag tgttcggaag attaacagaa gaatttcaga gataaataaa gagttggaaa
     3301 ttctcgactc cagaaataat gcaagagaaa ttcgcagagg cgatagagaa aaacgccttc
     3361 aagcacgcct taattatgtt caaaaaacag caggagttgt caacacccta tctcctgaca
     3421 atcagaaaaa actgttatcc aatattgaga aagaaaaaaa atcactcaca caacaacatt
     3481 cctccttgct tctgaaagca gatcaattaa attactctat ttttcttcag caatttgagc
     3541 tctctttaca atctatgcaa cgtccttttc aagaagcaga cgcattgaaa gctattgtaa
     3601 aaagaatgaa agagcatttg aattacaaag aaaaagcggc atcgattcaa tcccgattag
     3661 ataatacagt gcttactatt ggtgaaaatt taagaagatt aacgcgactt aatactcaat
     3721 tgagttcctt gcagcttgct aatcctgatt taacaaggag gaatgagcgc ctggaggaac
     3781 aaaacagaga gctccttgaa tcgtataata gccatataaa aacacgaaat aaactgtttt
     3841 ttccaactct cgttataaca ggattatcct tgctcttttc tattccattg atattaactc
     3901 taactggaat catcccctat gctattgcac cagctgtatt gttaactttg gttattgctc
     3961 cccctgcatt gttattgctt gctggacttg gtgtcggtat tgccacaatc atttatgctg
     4021 tgaaagctta cttcaataat tcaacgattg aatcaaacca ggaaacaata gagagtaata
     4081 ggcggcaaat gggagcaaac caaaaggaaa tttacactct ggaaaatcaa acaattccca
     4141 atcttaaaaa agaattgtta gagaacgaag aaattaaaaa tcgcttaact gatgaattac
     4201 agtatatcga aaacctggcc gagcaagcat taaaacaagc aaacgaagta gagccatatg
     4261 tctactcagc tatgcctttc tttaaccccg aggttgttgt tcatcaacat ccttctgtag
     4321 gtaatggtat agtaactcct agcgcacccc cgatcgatcc cctctatcca gccgtccggc
     4381 attaatttcc agcctgggtt attaacacag gctatcatcc gggatgactt aagttgattt
     4441 cccggatgac attatagcta tcagtttctc tttaaataat taccacaggc aattcagacc
     4501 aggaagttgt atagcgagga gaacgggact gcgcccgcat agcccatggc ttcacatgcc
     4561 cttctgctgc caaacgcaag gtatggcggc catatttcgt gttgatttca tctaataccg
     4621 acatcaatcg ctctgtctga ttcaattctt tctctgaaac ctggttaaac aaatctaact
     4681 gcaaacagtt tttttcaatt aaatctccta aataaacgcc taccttttgg taatggaatc
     4741 cagagcgaaa tattttacgc aggcacaatt tggcacaacg aatcaaatgt cgcagatcat
     4801 cagttggatt aatcagctta aaccctatag tttgctgata ttgtggcaaa tccaaacgaa
     4861 attgattggt tttgataaat atggaaagat ggcatgccat caatttttga tgacgtaact
     4921 tttcccaagc tctggcacaa tggctgctga tggcctgagc aagagcagaa tactctgttt
     4981 gcatagaacc aaaggatttg gaagatatta tgctttttct tgactcaaaa tcctgtaaac
     5041 tgccacatga aaccccacgc aactcaagca ctgttcgttg caatacaaca ctaaataatt
     5101 ttttaattaa ttgcaaatcc ataccagcca agtcatgagc tgtataaatc ccctgttgta
     5161 ctaacttttt accccattgc cgtcctatcc cccatacctc gccaacaact attttagcta
     5221 gccaatgata ttgattttca atgttaaata caggggttct taattccttt ttcgcaatat
     5281 ggtttgcgag tttagccagg gttttggttg cccctatgcc tatggatacg ggaataccgg
     5341 tatggcgcaa aacaattttt tgtaacttgc ggcaaaactc atcatgttca gattggggca
     5401 aagtattcaa atcaagaaaa gcttcatcaa tggagtaaat ctctattgct ggccaattgt
     5461 cttcaagaac tgacataacc cggcgtgaca tatcagcata caatgtgtaa ttggatgaaa
     5521 acacatgcac tttatgctgc ttacataatg ctttgactgc aaaataaggc actcccattt
     5581 gaacacccaa agcttttact tcattagatc gcgcaatcac acaaccatca ttattagaca
     5641 agaccaaaat gggtttattc gctaaggctg gtttaaataa acgctcacac gagacataaa
     5701 aattattaca atcgaccagc gcaaacatca caataccgta tgaataacat gagtgacgac
     5761 tccccaaatc accatatcgt attcctctgt aatatctatg ggaggaaaat cagggttttc
     5821 agccagcaac ttaacttgcc catgttgacg ggataaacgt tttactgtca attcgccatt
     5881 cagggcaacg attacaattt ttccatgagt agcctctatg ccacgatcaa cgattaaaat
     5941 atcaccggag taaataccgg catttatcat ggattctcca gaagctcgta caaaaaatgt
     6001 ggatgcagga tgcttgatca ggtactcatt caaatccagc atggattcaa tatagtcatc
     6061 acccggagaa ggaaaaccag ctcttacaga actggtatat aaaggaatcg ctgggaactc
     6121 cttttctcct tgaagatatt tcatcacatc ctgtatacga gattcaggta tacggactga
     6181 tttggttgat tctccatatt taccttgccc acgagggcgc ccagcgcctg ctcgtgcacc
     6241 accatgagac atatactccc ctaaacttga atttagaatc aaaattcaag tttaatttta
     6301 aagtagccca ttgtaaagtt ccgacaataa tgagagtttc atatgacgac taaactgaat
     6361 tgacgccaat actcaaatta cgttattgtt ggggatttag tgtaagacta aagagccatg
     6421 gttaaattat ataaggaaat aatcaattta atagataatc accaggcaac tatggtggag
     6481 caactccatc aattttgtga aatcaattct ggaacaacta acttgacagg attggccatc
     6541 atggctgaaa cgttgtgttc agctttcaaa ccgatcgctg ataccacaga attaataaca
     6601 ttaccgcctt tgtctgtgat tgacatgact ggaaacacag ttatgcaatc ctgtggggat
     6661 gtattgttca ttagcaaacg tcctcattta aaacgccgaa ttctgctaag tgggcatatg
     6721 gatacggttt attccgccag caaccctttt caaaaattaa gttacctgga tgctaatcag
     6781 atcaatggcc cgggggttgc tgatatgaaa ggcgggctaa tcgtcatctt gcacgcatta
     6841 gcggcctttg aaaacaccag atttgcggaa gatatgggtt gggatgtgtt aattaattca
     6901 gatgaagaaa ttggttcacc tgcatcaggt tccttattca ataaattggc acatcgctat
     6961 caggccgctt tggtttatga accagcaatg acacctaatg gtactttggc aaaaaaccgc
     7021 aaaggcagtg gaaaactaac actggtagca acaggaagag ctgcccatgc tggccgggca
     7081 tttgatgaag gaaggaacgc catatgctat cttgctgaag caattacggc tgttcatgcc
     7141 ttaaatgggc aaaaaaatgg agtaacaatc aatgtaggca aaatagctgg cggagaagcg
     7201 ctgaatgttg ttcctgataa ggcagtagct cagttggata tccgaattag tctgccagaa
     7261 gatgaaattt gggtaagaaa tgaattaaat aaaatcatca aacaattaga gcgacctgat
     7321 tattccctaa atgtgcatgg cacctttgga cggcctgtga agcgtatctg cgcagggaca
     7381 gaacgacttt ttcatcggat tcaaaaaatt ggagaggcgc ttggcttaac tattgactgg
     7441 aaagacagtg gaggttgttg cgacggtaat aatttggctc actatggctt gcctgttctg
     7501 gatactctag gtgttcgggg aggcaatata cacagttcag atgaatatat attgcttgat
     7561 agcttatcag agcgcgccgc acttagcgcg cttcttctga tagatttggc acagggtggt
     7621 ttagaggatc taataaaatg atgttatttc gtagcgctcg cgactctgac ttggatgcga
     7681 tccatcaact ggcagaaatc agtggtgttg ggatgacaac gctccctaaa gataaagaat
     7741 tattaaaaaa acgcttggaa tggtcttgcg aatcctatca aagaaatgtc actaaccctg
     7801 gctgtgaata ttattttttt gtcctggaag aacaaaaaaa taatacaatt gtcggtacct
     7861 ccgctataga agcaagaaca ggttatgaaa taccttttta ttcttataaa ctctctaaaa
     7921 gaaccagagt ctgtcacaca ctcaacatac gcagtgatta tgaagtatta agtttagtca
     7981 atgacaatca aggtcgcaca gaaatatgta ctctgtttct tcatcctgat tacagaaaaa
     8041 ataacaatgg cctattgctt tcgaaaggac gctttttatt tatggcacaa tatcctcatc
     8101 gcttcgcccc gattgtcatc gccgaaatgc gtgggatctc tgacgataaa ggcaactcgc
     8161 cattctggga taatgttgga gctaattttt ttcatatgcc ttttgtagaa gcggatcgtc
     8221 tgacactagc gacgaacaaa caatttatcg ccgatttaat gccccgcaat cctatctatg
     8281 tcaaactcct ttcgccagag gcgcaagcag tcattggcaa accacatcaa tcgactatcc
     8341 ctgccatgaa tattctctta aaagaaggct ttcgttataa caactacgtt gatatttttg
     8401 atgccgggcc tactatcgaa tcaccattat tagaaattaa aaccatttct ttaagccgga
     8461 tcatggttat taaaaacatc agtgatgaag cgagcagtag caatcgctat ttactcgcca
     8521 atacccaaat ggattttcga gcaacaatta gccacgctgt ttttaatgaa cgagaaaaca
     8581 cctgcattat tagcaaggaa acagcaaaat tgcttgatgt aaaacaagga gacagtctac
     8641 gtatatcacc catacgtaca gaagatactc caccattgta accaaggaat aatgatgtct
     8701 aaattacaaa tcatacagag taaaggccaa tatattaatg gagaatggat aaagggaaat
     8761 ggtctcattc tggagtctac aaaccccgcc tccggaactt tgctttggca agggaataat
     8821 gctacagatg aagaaattgc aaacgcttgt tacgttgcgc atcgcgcact gaaatcctgg
     8881 gctaatacca gttttgagga aagggctcgt tacactaagg cttttgtgga acaagtagaa
     8941 aaaaatcgtg agcaactggc acgacttatt tcattagaaa caggaaagcc attatgggaa
     9001 tctcaaactg aagtcagctc agtaattggc aaagtaaatt tgtcaattca ggcttatcaa
     9061 gaaagaacct ggccaaaaca aactgaaact gcagaagcta atgcctgcct ccgttttaaa
     9121 ccgcatggta ttgttgtagt attaggcgcg tttaatttcc ctgcccattt aagcaatgga
     9181 cacattgtcc ctgccctcct tgcagggaac acggtattgt ataaaccaag tgaacatact
     9241 ccagcagtag cggaattgat catccaatgt tggcacgata gcggcttacc tcccggagtt
     9301 atcaattgtc tacagggtaa tgcaaattgt ggaaataccc tattgtctca agatattcaa
     9361 ggcgtctatt ttaccgggag ttatgcaaca gggttgcgta tacaccaaca attctgtaat
     9421 agacccgaag ttattttagc tttggaaatg ggcggaaata accctttagt catcgatgaa
     9481 gtgaaagaca ttgatgcagc tgtataccat acaatccttt caactatgat cactgcagga
     9541 caacgttgca catgtgcgag acgaattata attcctgact cccagacagg cgatctattt
     9601 ttagaacgct ttgccaaggc ttgtaaatta atgcgaattg gatcctttga tagtcagcca
     9661 gagcctttta ttgggcctgt tattagccat gttcaagcat taaaacatct acacgctcag
     9721 aagcaactaa tcgaaatggg gggtgaaatc attctgccga tgtcgcttct tcttgagtac
     9781 acgggacttg tttctcctgg cattattgat atgactcgcg ccaagaaccc gccagatgaa
     9841 gagatttttg cgccatttgc acaaatttac cgttataatc actttgatga ggcaattcaa
     9901 ttagcgaatc agacacgtta tggtttgtca gcaggcttac tcagcgataa taaagaccat
     9961 tatcagcagt tttatcaaaa tattcgtgcc ggactaatca attggaacag accaaccacg
    10021 ggtgctgcca gtagccttcc atttggtggt gtcggatgca gtggaaatca tagaccaagc
    10081 gcctattttg ctgctgatta ttgtgcttat cctgtagcaa gcatggagca acctctgtta
    10141 acaactccag tgcaacgatt gccaggtctg gtattggagt aattattatg aatgtttatg
    10201 aattgaatat ggatgggctt gttggtcaaa ctcatcatta cgcagggctc tcctcgggta
    10261 atatagcttc tacaaataat gctttatcta tttccaaccc tcaagctgca gcaaggcaag
    10321 gtttggaaaa aatgcggcaa ctttataaca tggggttaaa gcaaggccta ttgcccccac
    10381 atcaacgacc taatttgaat ttactgtatc aactgggatt taaaggcact cccagtgagc
    10441 agataaacaa agcctataaa acagcgcccg aattgcttag tgcctgctac tctgcctcaa
    10501 gtatgtggac agctaatgca gcaactgttt ctgcaagcgt ggatactgaa gacaacaaag
    10561 tacatttcac tgctgccaat ttaatcagca atttgcatcg tcaccaggaa gctgattttt
    10621 caaaaaaatt attggaattt atcttttcca attctgatta ttttaatcat caccctctgt
    10681 tacccaaatc aatgggcaca agcgatgagg gagcagctaa tcataatcgc ttatgtcaaa
    10741 gtcatgcgca ctcagggatt aatctctttg tatacggtaa aaaagtgctg ggaaatcatc
    10801 aattcgaaca gagcccaata aaataccctg ctcggcaaac taaagaagcg tcagaagcca
    10861 tcgctcgcaa ccatttatta aatcctgaac gagtaatctt tgcctgtcaa aatccattag
    10921 caatagatca aggtgtattt cataatgatg tcatctcagt tgctaatgaa catgtatttc
    10981 tggttcatga agaagctttt tataatcagg cttatgtttt ggatcaatta agagaaaaag
    11041 ccgattttcc tttagtaata attcagatct caaaagaaca aatcagcgtt agtgaagcag
    11101 ttgataccta tcttttcaat tctcaattga ttaccctgcc tgatcagaag aatatgattt
    11161 taatagcccc tgctgaatgt caggcaaacc ttaaagtaaa aacttgtatt gatggattgg
    11221 tcgccgatcc tcaaaaccct attaactcag tgtattacct ggatctgaaa caaagcatgc
    11281 gtaatggcgg aggaccagcc tgtttacgct tgcgagttcc tttgaatgac tacgagctta
    11341 aagcaatgca ccaaggtatt ctaattgata atgatttgtt agatatttta gataaatggg
    11401 ttttaaaata ttaccgaact gaactgaaaa tccccgattt agctgatccg caactattat
    11461 atgaatgtct ggatgcattg gatgaattaa ctcagatctt gaaattaggc tcaatatatc
    11521 cctttcaatc ttgagtatcc gagtcatgtg gaaaacaaaa ttcggcaaat gtatctatat
    11581 ttcaccgtca ggatataaag tctatcaaaa tttgttgtac cgttggctta ctttaggtag
    11641 cgaagcatgg caaactgtaa ttaaccgtta tatacctcgc aggcctggtt tacattatct
    11701 gcaaattctg acacttatgg cgcgaaaatt tcctaatgat tgttgtttat taggacttgg
    11761 aggagcagga atcgctcacc tgctgggtgc acaaaaaacc atacgctcta tcgttgccgt
    11821 ggaaagtagt gaagaagtga ttcaaattgc caaaaaattt ttcatgacag atttaattcc
    11881 tcatttcacc attactcacc aaaacgcccg agattttgta cgtgaaaaca aaaagacttt
    11941 tcagcacatg cttcttgatc tatatgacgc aacacatttt cccgatgagt gtcattctat
    12001 ggatttcttc attcattgta aaaaacaatt aactgacgat ggatttttag ctgttaactt
    12061 agcaaattcc caagaacaat gggctctggt ccaactaatc aagaaacaat ttccatccaa
    12121 tatcattatt ccaataaaaa aatgtgccaa tattgttgtc attgcctcca aaaatgacaa
    12181 caaagacttg tttattaaac aagtcaggaa tactaatgaa ataaaaaaaa ttacatgggt
    12241 aaaagattgg ggttatgttg ctgaatatta atgttcattg aaaggaaaca atagtttatt
    12301 tacaaatgaa acccagccaa aagctggtat gtatttaaat attttatctt attttcgcat
    12361 gtaattaatc gcttcaatta atgcctcatc agagcaatcg gtattaggga catctgcctc
    12421 tttacataaa ccgccatgat gcatcaaccc tttacccacc ttgacattgt acagcaggta
    12481 atccattgcg ctttcaaatt gtgaaggatt attttttgct tctatttttg cttgtttaaa
    12541 tcgcagcttc catgcctttt tatcaaaagc aggaggggct tttatagcct tggctaaatt
    12601 aggggcatga caatttttac aggcaatttc gtaagtatat tttcctaaat ttttagcaaa
    12661 aactggagta cttgttaaat aaactaacat tataatcaat gcattccttt tcatcacatc
    12721 cccaaattat tattgctaaa gcaatcttag gattatatga gatattttgc caattgaaat
    12781 agttttttac tttttaatta aagcgtatag accattatct tgatgcttaa ttatagaaat
    12841 accctaacca atttattggt actgaagaga tatgaacaaa aatgaaacaa atatgctaaa
    12901 agtcaaggca agatcattcc tattttagtc gattatgccc gtgatatact ttataaattt
    12961 gagcatattt ttgtcattct cgcacgg
//
LOCUS       DATNSN010000098        12933 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.098, whole genome shotgun sequence.
ACCESSION   DATNSN010000098 DATNSN010000000
VERSION     DATNSN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.098"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1265)
                     /locus_tag="VAH76_002124"
     CDS             complement(<1..1265)
                     /locus_tag="VAH76_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1386731.1"
                     /translation="MVMAATIPHIETITFTDWFLAIIDTPGIPIDEIETRAAEGFKRK
                     LTERQFQQLTELIHLISFVKHRRFSNPTLALRIYIDENTSSLSRTALVEAVRMLPPED
                     NKTYELVACLAQKNKLERYTSITKVPPLQIYQGDGVFEQYDEWILLFELFELTQATPS
                     DFIPAIAHLLIIKNFGIPDLRALSKFMSTTHKLGILSQNFMNNMTPHLCNGQIMEKYE
                     SLLLKLQINDLLSEEILEVIYPLLNQPDALDAFFSLYHEELSHDSALSFLREILPPFC
                     AMSLPSKENYDDQVPTNTPLHLAVIESDKGNLERALAFANRNLLIKCSYENTALLLAC
                     KLADKESARLILLKMQELGCDVNQRDHHGMTALHWANFYHFDELIRELEFAGADPQLK
                     AANGKTCEYFYQHQFTLKDLKLNGKEIIDG"
     gene            1522..2004
                     /locus_tag="VAH76_002125"
     CDS             1522..2004
                     /locus_tag="VAH76_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HEO1386732.1"
                     /translation="MKFRFRFYLGIILLILAIVCPLLIPLIVQSNFSVLMKGFLSSIL
                     VFGLPEVLIVLAIALLGKEVYGLLEQKIKNILFKEKVSRARYRLGLVFFAFPLIIGLL
                     EVFVKEITLAYGSYYYWVEIIWTTMFALSFFICGKQFWDKFKSLFIYDSYVVFEKNKK
                     "
     gene            complement(2026..3021)
                     /locus_tag="VAH76_002126"
     CDS             complement(2026..3021)
                     /locus_tag="VAH76_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEO1386733.1"
                     /translation="MDPITHGVLGAACAQAAFGKFDKRIPWQAGALAGMAPDLDVFIR
                     FSQEPLSLEQWHRYLTHSLMFIPIGGLVVALFLLCFSQYRQYWKVTIGASLVGYATHC
                     LLDSLTSYGTVLFWPWSDSRISWDIISIIDPVFTLPLLLGTAWSVIYQNQKAVTASLF
                     FCILFLIFNTILHNRAVDGIHQYATSNNFKINHPRALPKLASSTDWRIIAKQQDCLII
                     AEAKTSLFYPTTIRLIRKALSFNESMIPFALSSGQKSDLATFHWFTDGFLIVANKNPL
                     ILADARYTIDDSPIFSLWGIEILPGQEHINKLSLPKINEYCKSPDSESYNENGLN"
     gene            3225..5441
                     /locus_tag="VAH76_002127"
     CDS             3225..5441
                     /locus_tag="VAH76_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386734.1"
                     /translation="MIRTFLTAFLMLFCSFALAQEKVVTHFFKEVNSSIAISEGKANI
                     QASQYRVVDIDINQLYAELENAPHRDGISTGISLQFELPQPDGTVKRYQVMENSTLAP
                     ELSTKFPEIKTYDAYGIDNPGELVKFDLTPQGFHAMILSPGRDTVFIDPLIKGNTQYY
                     MIYYKKDFITSKKMKCGVKSQNQPLINAASKRGFTDFNPCVLKKYRLALAATAQYTQF
                     HGGTVPQALAAETTTVNRVNGIYEIDMAITMQIIANNNLIIYTDPNNQPYTSGDPDKM
                     INENQANIDQMIGSANYDIGHVVDAAGSGLAQTPSVCINGEKAMGVTGQSNPVGDPFD
                     VGYVAHEIGHQFGANHVQNNNCQRNNPTAVEPGSGSTIMSYAGICDPNVQDDSDPYFN
                     GISLQEMGNFVSDAAHTCPVKTSIPSAPVIQGTNGGVKIPAQTPFALTATATKSGGNE
                     VLTFAWEQQNNEASQQPPVSTSRVGPNFRSFSPQVSGTRFFPNLNALANNGPFTWEVL
                     PSVSRTLKFRVTVRRNTPGGSCNAYTDTTLSVESKAGPFVVTNPTESGITWTGISPRT
                     VTWDVANTNIPPINARFVNILLSIDGGQTFPFTLLSNVDNTGSEVICVPNLNSSTARI
                     MVQASNGTFFNVSKNNLTIIPVPPRPPELTRADRNPMNTSEAFVLYANCIPTGNDVYT
                     VNGLPPGATVRFDANNRRFVIENINTPKRVRNVTITATDENGVNRTSNAITIPSIL"
     gene            5454..6155
                     /locus_tag="VAH76_002128"
     CDS             5454..6155
                     /locus_tag="VAH76_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEO1386735.1"
                     /translation="MNRYSIFFLVFCAFSLESHAGLYSGINLGINAVKINKDLVYPLE
                     DPVPTTSSFNSAYTDFHGQLIAGYDFSLTSKISAAVEANIDLFTGKARHRITEWYFDE
                     DVYAEEQLKVGYSLFLLPTYHLNDMVQLFVGPGVSSSRFNIAFSNTAGNVGVSDSLNE
                     WLTGVSIKAGSITKLTRNIDLLLTYQFTQYNSVTGVQMEPLSEDTLRGSYKPNVNTVL
                     IGFKVHPSDEITKDT"
     gene            complement(6224..6415)
                     /locus_tag="VAH76_002129"
     CDS             complement(6224..6415)
                     /locus_tag="VAH76_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386736.1"
                     /translation="MECKCNQCGMGVKNLTCSKCDALLVSGTIVKNDGNKVQVAKCPN
                     GCGQIKSPVCCGHDMACSV"
     gene            complement(6470..7330)
                     /locus_tag="VAH76_002130"
     CDS             complement(6470..7330)
                     /locus_tag="VAH76_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386737.1"
                     /translation="MISYLFSEVHFTHALKKVLGISVLVGVYSAIIYFWFEQDNLHSS
                     NWLSYSQSVFGIILGLLLVFRSNRAYERWWEARTLWGQLVNASRNLAIKIRVLLSPNT
                     DEAKKFSELINTFCEVLAIHLRQKSTRDDFKKFLKMVSLPSHVPSYVAQELYCELSEK
                     TKASKRLNLWLLDNEFSKFMDICGGCERIKNTFISLSFRVFVKHVFIIFILFLPCSLI
                     DTLGVWAIPATIIITYLIIAIEGIARNLEEPFGLTEDHLNLGAITESIRESVNEVLLG
                     QKSNEESNSI"
     gene            complement(7507..7743)
                     /locus_tag="VAH76_002131"
     CDS             complement(7507..7743)
                     /locus_tag="VAH76_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386738.1"
                     /translation="MKRVHHHPATHSHPSNATVQVSNGQQTVVVNPNPPYFPGQPPMY
                     HPNSFFTTHHHSPYYASQNQHYHQQPAPNTHQHP"
     gene            complement(7946..8158)
                     /locus_tag="VAH76_002132"
     CDS             complement(7946..8158)
                     /locus_tag="VAH76_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0045 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386739.1"
                     /translation="MKSTNNIQKKETDKPTSEQAQKKEVRTNNAPDFIPGASPYYNLY
                     CFFYKLCHGEDTSRQHQPATSFQHGK"
     gene            8368..8766
                     /locus_tag="VAH76_002133"
     CDS             8368..8766
                     /locus_tag="VAH76_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386740.1"
                     /translation="MSCFPFFAKKNKKNNEEKAEKTLSVFPAGTSINKTTVKEICFTD
                     IFCDTHPFVWYYYPEHNLAIWTSRLAGTTQEYPVDYLLNQLAKIPGITIDQGDAAIKD
                     VSTFLQDHQMIPIRNSESWRYIKSSSFSMS"
     gene            8965..9627
                     /locus_tag="VAH76_002134"
     CDS             8965..9627
                     /locus_tag="VAH76_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO1386741.1"
                     /translation="MKPTDKKHWSKIEYLHQSIKNPNIHIKGKHSYYSDAWTGSFEET
                     VVRYLYGDEYSLKTWQPQWQIDQLYIGDYVCIAAEVIILLGGNHNHRADWFCLYPFAD
                     KYVEAYQGKGDTIIKDGVWLGMRAVIMPGVTIGEGAIVAASSIVTKDVEPYSIVAGNP
                     AKPVKKRFAENVIERILALDIYSWSEAKFNLLKDYICSNNIDKLEQASRSYDQNHTIE
                     SK"
     gene            9624..10502
                     /locus_tag="VAH76_002135"
     CDS             9624..10502
                     /locus_tag="VAH76_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386742.1"
                     /translation="MINIRKLRLGDESTLETFLKSHADESMFLRSNLYHSGLSYHDKP
                     FHGDYFAAFDNTKKISGVLAHYWNGNVIVQCPDFNVLTALVGAFQKTATREIAGILGE
                     DQQADVVMKQLQLEHVDYAVNYPDGLYALNLQNLAMPDNQDRYQLKVAKDCDKCLLYE
                     WLRAYQIEALGAEEGSSILKEQVESEVKNTLEGNDRWVLEENGIPVSLCGFNARLPDI
                     VQIGPVWTPVTLRGRGYARTVVALSLSAAQKSGVSRAVLFTNNQAAVRAYQAIGFNKV
                     GNYRLAILKKPILLTR"
     gene            10555..11850
                     /locus_tag="VAH76_002136"
     CDS             10555..11850
                     /locus_tag="VAH76_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1386743.1"
                     /translation="MDMDIKHKPLKRELSLFGATMMGLGSIVGTGVFVSIGIAAGVTG
                     PSVILAIALAALVATCNAFSSAQLAANHAVSGGTYEYGYRYLHPAFGFTAGWMFLCAK
                     SASAATAALGFAGYFIHLSGLETVPVIPIALAVSITLILLVLSGLRRTNTVNIIIVMI
                     TLITLTVFIISGLPDFMQRGTANLRPFFPVSKQEGLSHFFYATALMFVAYTGYGRIAT
                     LAEEVKNPGKFIPAAIIITLMVSAVLYILVGLVAIGAVGVEKLAQVTETNATPLESAA
                     QAIGIPGLSILVSIGACTAMLGVLLNLILGLSRVALAMGRKGDLPKMFCYVSETRHVP
                     VSAVIGVGVVIIALVLTGSVETTWTFSAFTVLIYYAITNLAALRLPKEEQLYPPLFAI
                     GGLLSCLFLAFWVPIGVWITGISLLVLGLLWKVVANRFL"
     gene            complement(12067..12219)
                     /locus_tag="VAH76_002137"
     CDS             complement(12067..12219)
                     /locus_tag="VAH76_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386744.1"
                     /translation="MFNLKRNLIKLGTLSISIALLAACHTVKGTVTGAKQDIKATGKA
                     ISRTMQ"
     gene            complement(12494..12880)
                     /locus_tag="VAH76_002138"
     CDS             complement(12494..12880)
                     /locus_tag="VAH76_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEO1386745.1"
                     /translation="MRVGNTRYNFETAFDYIFVFVIGAVLSRAINGNSTLISAMAGSS
                     LLIFLHWVFAICSFYSHGFGKLVKGKSVLLIQDGQLIWSALKRHQVTEEDLRQVFREK
                     LNASDLTTVKEARLERTGRISFITYK"
BASE COUNT         3976 a         2286 c         2595 g         4076 t
ORIGIN      
        1 ccatcaatga tttctttgcc atttaatttt aaatctttca aagtgaattg atgttgatag
       61 aaatattcac aagtttttcc attggctgct tttaattgag gatccgcccc agcaaactcc
      121 agctccctga ttagctcatc aaaatgataa aaattagccc agtgcaaggc ggtcatgcca
      181 tgatgatctc tttgattgac atcacagcct aattcctgca ttttcaataa aatcaatcgg
      241 gctgactctt tatcagcaag tttgcaagct aataaaagtg ctgtattttc ataagagcat
      301 tttattagta aattgcgatt ggcaaaggct aatgctcgtt ccagattccc tttgtcactc
      361 tcaataacgg ccagatgcaa aggtgtgttt gttggtactt gatcgtcata gttttcttta
      421 ctggggaggc tcattgcgca aaagggtggc aaaatttctc tcaggaaaga cagagcgctg
      481 tcgtgcgata gttcttcatg atagagtgaa aaaaaagcgt caagagcatc cggttgattg
      541 aggagcggat agatgacctc tagaatttcc tcgcttaaca aatcgttaat ttgtaattta
      601 agcaataatg actcgtactt ttccatgatt tgcccattac ataaatgggg tgtcatatta
      661 ttcataaaat tttggcttaa tatgcctaat ttatgcgtag tgctcataaa ttttgaaagt
      721 gccctaaggt cagggatgcc aaaattcttg atgattaata aatgagcaat ggcaggaatg
      781 aaatcgctgg gcgttgcctg ggttaactca aacagttcaa aaagaagaat ccattcatcg
      841 tattgctcaa atacgccatc cccctggtaa atttgcagag gaggtacttt cgttatgcta
      901 gtatatcgtt ccagtttgtt tttttgagcc aagcaggcta ccaactcata ggttttatta
      961 tcttcaggtg gaagcatcct gactgcttca accaaagcag ttcttgatag agagctcgta
     1021 ttttcatcaa tatagatgcg gagagccaag gtaggattgg agaaacgcct gtgcttaacg
     1081 aaactgatta aatggattaa ttcagttaat tgttgaaatt gccgttctgt caattttctc
     1141 ttaaagcctt cggccgctct tgtttcaatt tcatcgatgg gaatgcctgg agtgtcaatt
     1201 attgccagga accagtcggt aaatgtgatt gtttcaatat ggggaattgt tgcggccata
     1261 accactcctt aaataaattg ttaatattat aaacaaaaag cattattttg ataacagtga
     1321 aatgacctct tgaaagtaaa tatgataata gagttgaaaa agtcatagtg tcggatttca
     1381 tagcatctgg tggttatagg gaaaaagcgg gtgagcgagg ctttatttaa taattgcagg
     1441 aattaatggt tcattttgta gatattcaat cttcttctgg attactattg ataagtaagg
     1501 taaatagtgt gagtagctat cttgaaattt cggtttcgtt tttatctggg tattattctt
     1561 ttaattttgg cgatagtttg ccctttgctg atcccgttaa ttgtgcaatc caacttctct
     1621 gtgctgatga agggatttct atcttcaata ttagtatttg gtcttccgga agttttgatt
     1681 gtcttagcta tcgctttact tggaaaagaa gtgtatggcc ttctcgagca aaaaattaaa
     1741 aatatccttt ttaaggaaaa agtatcaaga gcccgttacc gcctgggttt ggtattcttt
     1801 gcttttccac ttattatagg gttgctggag gtgtttgtaa aagaaattac cttagcctat
     1861 ggaagttatt attattgggt agaaattatt tggacaacga tgtttgcttt aagctttttt
     1921 atttgcggga aacaattttg ggataaattc aaatctttat ttatttatga ttcttatgtt
     1981 gtttttgaaa aaaataaaaa atgagcttat taattgtaat tgtttctagt ttagtccgtt
     2041 ctcattatag ctttcgctat ctggcgattt acaatattca ttgattttag gcaagcttaa
     2101 tttattaata tgttcttgcc cgggcaatat ttctattccc catagtgaga atatggggct
     2161 gtcatctatc gtatatctcg catctgcgag gattaaggga tttttattgg caacaattag
     2221 aaatccatcg gtaaaccagt gaaatgtagc caaatcactt ttttgtccgg atgacaaagc
     2281 aaaaggaatc attgattcat taaagcttaa agcttttcga atcagtctga ttgtggttgg
     2341 atagaaaagg gaagttttcg cttccgcgat aattaaacaa tcttgttgct tggctatgat
     2401 gcgccaatca gtagagctgg ccaatttagg cagggcacga ggatggttta ttttgaagtt
     2461 attactcgtg gcgtattggt gaatgccatc tacagcgcga ttatgtaata tcgtattaaa
     2521 aatcagaaat aaaatgcaga aaaacaagct tgcagttact gccttttgat tttgatagat
     2581 gactgaccag gctgttccta acaatagagg taaggtaaat acgggatcaa taatagaaat
     2641 aatatcccaa ctaatacgac tatcgctcca gggccaaaat aaaacggtac cgtaagaagt
     2701 caaggaatct aataaacaat gagttgcata accaacaaga gaggctccaa tggttacttt
     2761 ccaatattgt ctatattgtg aaaaacacaa tagaaacagt gcaactacca ggcctccaat
     2821 tgggataaac attaaggaat gcgtaaggta acgatgccat tgctcaaggc tcaatggctc
     2881 ttggctaaaa cgaataaaaa catccaaatc cggagccatc cctgctaaag cacctgcttg
     2941 ccaggggatt ctcttgtcaa attttccaaa agccgcttgt gcacaagcag cacctaaaac
     3001 accgtgagtt atagggtcca ttttttactt atcaggctgt tgatacaatg aaaacacgct
     3061 tcttctgaga aagattttgc tttatttcat tgaaaaaatg agtattataa gctgcaaaag
     3121 aatttttata gatatttttt aaaaaataaa agtaattttt tcaattttaa ttgaacttac
     3181 tgttctaatg ataattgatt ttactcaact tgatgggttt tacaatgata aggacgtttc
     3241 ttacagcatt cctcatgctg ttttgttcat ttgctttagc acaagaaaag gttgtcactc
     3301 atttttttaa ggaagttaat tcttccatag caatttcaga aggaaaagcg aatatccagg
     3361 caagccaata tcgtgttgtt gatatcgata taaaccaact ttatgcagaa ttggaaaatg
     3421 cgcctcatcg tgatggtata agcacaggca tatcccttca atttgaactt cctcagccgg
     3481 atggaactgt aaaacgttat caagtgatgg aaaacagcac tttagccccc gaattaagca
     3541 caaaatttcc agaaattaaa acatatgatg cctatgggat tgataatcca ggggaattag
     3601 taaagtttga tctgacacct caaggttttc atgccatgat cttaagtccg gggagagata
     3661 cagtctttat tgatccttta ataaagggta atacgcagta ctacatgatt tattacaaaa
     3721 aagatttcat tacttcaaaa aaaatgaagt gcggtgtcaa aagtcaaaat caacctctta
     3781 taaatgcagc ttcaaaaaga ggtttcactg attttaatcc ctgtgtgtta aaaaaatatc
     3841 gtcttgcatt ggcggcaacg gcacaatata cccaatttca tgggggcaca gtacctcaag
     3901 cacttgctgc ggagacgact actgtcaaca gggtcaacgg tatctatgaa atcgatatgg
     3961 caatcacgat gcaaattatt gctaataaca atttgattat ttacacagac cccaataacc
     4021 agccttatac cagcggcgat cctgataaaa tgattaatga aaatcaggca aatatcgatc
     4081 aaatgattgg atcagctaat tacgatatag gccatgtggt tgacgctgct ggcagtggat
     4141 tggcccaaac tcccagcgtg tgtataaatg gtgaaaaggc aatgggtgta actgggcaaa
     4201 gcaatcctgt tggagaccca tttgatgtcg gttatgttgc ccatgaaata ggacatcaat
     4261 ttggtgcgaa tcatgtacaa aataataatt gtcaaagaaa taatcctact gctgtagagc
     4321 ctggaagcgg cagtacgatt atgagctatg cgggaatttg cgaccctaat gtccaagatg
     4381 attctgatcc ctattttaat ggtattagtt tgcaagaaat ggggaatttt gtttctgatg
     4441 ctgcgcatac ttgtcctgta aagacatcaa taccctctgc tccggtaatt caaggaacaa
     4501 atggcggggt taaaatacca gcacaaacgc cttttgctct aactgctaca gcaactaaaa
     4561 gtggaggtaa tgaggtatta acttttgcct gggagcaaca gaataatgaa gcatcacagc
     4621 agcctcctgt tagtacctca cgagttggac caaactttag aagtttctct cctcaagtat
     4681 cggggacacg ttttttcccc aatttaaatg ccttggctaa taatggtccc tttacctggg
     4741 aagttttacc ttcagtttct cgtaccttga agttcagggt aaccgtacgc aggaatactc
     4801 ctggaggttc ttgcaatgct tatactgata ctaccttatc tgtagaaagt aaggctggac
     4861 cttttgtagt gacgaatccc accgagtcag gtattacctg gactggtatc tcaccaagga
     4921 cagtcacttg ggacgtagcc aatactaaca ttcctccaat aaatgcccga tttgtaaaca
     4981 ttctattatc aatagatgga gggcaaactt ttccttttac cctcttatca aatgtcgaca
     5041 atacaggaag tgaggtaatc tgtgttccta acctgaactc gtcaacagca agaattatgg
     5101 tgcaggcatc aaatgggact ttttttaatg tttccaaaaa caatttgaca attattccag
     5161 ttccacctcg accaccagag ttaacgcgcg ctgatcgaaa ccctatgaat acttctgagg
     5221 cttttgttct ctatgcgaat tgcattccga ctggcaatga tgtgtatacg gtgaatggat
     5281 tgcctcctgg tgcaacagta agatttgatg caaataatcg acgatttgtc attgagaata
     5341 ttaatacacc gaaaagagtg cggaatgtga ctattaccgc aacggatgaa aacggtgtta
     5401 acagaacttc aaatgcaatt actatcccca gtattttata aaaagggctc aatatgaata
     5461 gatattccat atttttttta gttttttgtg ctttttcact ggagtctcat gctggtttgt
     5521 attctggaat taatttggga atcaatgctg ttaagataaa taaagatctt gtctacccgc
     5581 tggaagaccc tgtgccaaca acttccagtt tcaatagtgc ttatactgat tttcatggac
     5641 aattgattgc aggctatgat ttttctctta cctcaaaaat atctgctgca gttgaagcaa
     5701 atatagactt gtttacaggt aaggcaagac acagaattac cgaatggtat tttgatgagg
     5761 atgtgtatgc agaagagcag ttaaaagtag gttattcgtt atttttgtta cccacctatc
     5821 atttaaatga catggttcaa ttgtttgtag gtccaggcgt gtcatcaagt cgatttaata
     5881 ttgcttttag taatactgct ggaaacgtag gggtcagtga tagtttgaat gaatggctaa
     5941 ccggggtaag cattaaggcg ggttcaataa cgaagctgac tcgcaatata gacttacttt
     6001 taacctatca attcacacaa tataacagtg ttaccggagt tcaaatggaa cctttatccg
     6061 aagatacgtt gagagggagt tataagccaa acgtaaacac agtgctaata ggctttaaag
     6121 tacatccttc agatgaaatc acaaaagaca cttaatctca gttatggata agacctcaat
     6181 tgcagttatt tgaggtttcc tatccttaac tgattttaaa taatcagact gagcacgcca
     6241 tatcgtggcc gcagcaaaca ggtgatttga tttgaccgca tccgttgggg cattttgcta
     6301 cttgaacttt attcccatca tttttgacta tagtgccact aaccaacaat gcgtcacatt
     6361 tactgcaggt taaatttttt acgcccatac cacattgatt gcatttacat tccattataa
     6421 tgactccctt ttatttaagt agaaaaactt ttattaatta taatccatac taaattgaat
     6481 tggattcttc attagatttc tgccctagca agacctcatt gacgctttct ctaatgcttt
     6541 ctgttattgc accaaggttt aagtgatctt ctgttaaacc gaatggttct tctaaattgc
     6601 gagcaattcc ctctatggcg atgattaaat aggtgataat aatggtagca ggtattgccc
     6661 aaactccaag cgtatcgatt aggctgcaag gtaaaaaaag gataaaaata atgaatacat
     6721 gctttacaaa aacccgaaat gaaagcgaga taaatgtatt tttaatacgt tcacaaccgc
     6781 cgcaaatatc cataaatttg ctaaattcgt tatccaataa ccatagattc aatcttttcg
     6841 atgctttggt cttctcagaa agctcgcaat acagttcttg tgcaacataa gacggcacat
     6901 gactgggaag actcaccatt tttaaaaatt ttttaaaatc atcccgagta cttttttgcc
     6961 tcaaatggat ggccaatact tcacaaaacg tattgatgag ttcagagaat tttttagcct
     7021 catcggtatt aggtgataac aataccctga tttttattgc taaattacga ctggcattga
     7081 ctaattgacc ccacagggtt ctcgcttccc accaacgttc ataagcccga ttgcttctaa
     7141 aaaccaaaag caaacctaaa atgatgccaa atacgctttg cgaataggat agccagttac
     7201 tggaatgcaa gttatcttgc tcaaaccaaa aataaataat ggcgctgtaa acaccaacca
     7261 aaacactaat tccaagtact ttcttcagag catgggtgaa atgtacttct gaaaataaat
     7321 agctaatcat ggcagtccat ttgattgttt cgtttttcca gaataataga aaccgatctt
     7381 tctataaagt gctttgtcaa aaaagtggga gcaaaataag taataaccag agaatgccag
     7441 ttattactta ttgttcagtc taagttctta aagagcaata gtctgtttat taacagttac
     7501 caccatttat gggtgttgat gagtattggg cgctggttgt tggtgataat gctgattctg
     7561 agatgcgtaa tagggtgagt gatgatgcgt tgtaaaaaaa ctattgggat ggtacatggg
     7621 cggttggcct ggaaaataag gagggttcgg attaacaacc acagtttgtt gtccattgct
     7681 gacttgaaca gtagcatttg atgggtgaga gtgtgttgca gggtgatgat gtactcgttt
     7741 catagattat tatccttata aaaatacaac tgcatgacat acataaaact gactttactc
     7801 aggttatttc agaatagcct gagttcgaga ttgtaatgta gatagttaaa caagtacaga
     7861 tagtattaaa aatttactat tttttgtttt tagatacatg caacgatagt aaatcctggc
     7921 taccccttat tatgttaaaa gaaatttatt tcccatgctg aaaagaagta gcaggttgat
     7981 gttgtctgga tgtgtcttcg ccatggcaaa gtttataaaa aaaacaataa agattataat
     8041 aaggtgatgc accaggaata aaatcggggg cattatttgt tctcacttcc ttcttttgtg
     8101 cttgttccga agttggttta tcagtctctt ttttttgtat gttatttgtt gatttcataa
     8161 atcttatcct tgattaagaa aatagattga attttaatta tcgttcaaac caggaaagag
     8221 agttccggtt tgtttacaaa tgaaccgttt tattgctcat ggcttggtga ttatagttca
     8281 gacaggtgat caattgtagt agttgtccca gatgtgttat ggttatctga ctgacaggaa
     8341 tcgagatgcc agaacaagga atagataatg agctgttttc ctttctttgc aaaaaaaaat
     8401 aaaaaaaata acgaagaaaa agcggaaaaa acattaagcg tatttcctgc aggtacttct
     8461 ataaataaaa caacggtaaa ggaaatttgt tttacagata tcttttgtga cacccatcct
     8521 tttgtttggt attactatcc agaacataat ctggcaattt ggacttcaag actggcaggg
     8581 acaacccaag aatatccggt agattatttg cttaatcaac ttgcaaaaat tcctggaatt
     8641 accatcgacc aaggtgatgc agccatcaag gatgttagta ctttcctcca ggaccatcaa
     8701 atgattccta ttagaaacag cgaaagctgg cgatacatca agtcttcttc tttctcaatg
     8761 agttaatgaa gagattgaaa gcaggccctt gttcttaata atggaagatt ttgctggctc
     8821 agtttcgcaa gtgatgcgca agggatattc aaatgatttc tctcatctta ttgattgcat
     8881 agagcccaca cataatgaaa cattagaaag ttatgatcat aataatatac aatgagatca
     8941 tttaaattcc taaatgagca aggcatgaaa cctactgata aaaagcactg gtctaaaatc
     9001 gaatatctac accaaagcat aaaaaatcca aacatccata tcaagggtaa acacagctat
     9061 tatagcgatg cctggactgg tagttttgag gaaaccgtcg ttcgctatct ttatggagac
     9121 gaatacagtt taaaaacctg gcaaccacaa tggcagattg atcaattata tattggtgat
     9181 tatgtttgta ttgctgctga agtgataatc ctgttaggtg gaaaccataa tcaccgagcg
     9241 gactggtttt gtttgtatcc atttgcagat aaatatgtag aggcttatca aggcaaaggc
     9301 gatactatta ttaaggatgg tgtctggctt ggcatgcgtg ctgttattat gccaggcgtt
     9361 actattggcg aaggagcaat agtggctgca agcagtattg taaccaaaga tgtggagccc
     9421 tacagcattg ttgctggaaa tcctgcgaaa ccagtcaaaa aaagatttgc agaaaacgtg
     9481 attgaacgaa ttctggcttt agatatttat tcctggagcg aagccaaatt taacttatta
     9541 aaagactata tttgcagcaa taacatagat aaattagagc aggccagcag gagctatgat
     9601 caaaaccata ccatagaaag caaatgataa atatcaggaa attaagatta ggtgatgaat
     9661 ccaccttgga aactttttta aaatctcatg ctgatgagag tatgttttta cgcagcaatc
     9721 tctatcactc cggtttgtct tatcatgata agccttttca tggggattat tttgctgcat
     9781 ttgataacac caaaaagatt agcggtgtat tagcccatta ttggaacggc aatgttattg
     9841 tgcagtgccc tgatttcaat gttttaacag cactagtggg cgcttttcaa aaaacggcta
     9901 cccgagaaat tgcaggtatt ctcggcgaag atcagcaagc cgatgttgtc atgaaacaac
     9961 tccagctgga gcatgtagat tatgccgtga attacccgga tgggttatat gcacttaatt
    10021 tacagaatct ggcaatgcct gataatcaag acagatatca actgaaagtc gccaaagact
    10081 gtgataaatg tttattatat gaatggctta gagcttatca aatagaagcc ttaggtgctg
    10141 aagagggcag ttcaatttta aaagagcaag tagagagtga agtgaaaaat actctcgaag
    10201 gcaatgatcg ttgggtttta gaggagaacg gcatccctgt ctcgctatgc ggttttaacg
    10261 caagactacc ggatattgtc caaattggac cggtatggac accagttacg ttgcgggggc
    10321 gaggatatgc tcgaacagtt gttgcgctta gtcttagtgc agcccaaaaa agtggagtga
    10381 gtcgagcggt attatttact aataatcaag ctgccgtacg agcttaccag gcaattggtt
    10441 ttaataaagt aggcaattat cgattggcca tattaaaaaa accaatactc ttaacacgct
    10501 aattgtgaat acctgttaaa acagtaaaag tatttattga taggaaataa aacgatggat
    10561 atggatatta aacataagcc tttgaagcgt gaactcagtc tatttggcgc tacgatgatg
    10621 gggcttggct ctattgtcgg gacgggtgtt tttgtcagca tcggaattgc ggcgggtgtc
    10681 acaggaccat ccgtcattct tgctattgct ttggcagcac ttgttgctac ctgtaatgca
    10741 ttctccagcg ctcaacttgc tgcaaatcat gctgttagtg gaggtactta tgaatatgga
    10801 tatcgttatt tacatcctgc ttttggtttt actgccggtt ggatgttttt atgtgcgaag
    10861 agcgcttccg ctgcaacggc agcgcttggc tttgccggtt attttattca cttgtcaggt
    10921 ttagagacgg tgcctgtcat acctatcgct ttagcagtgt ctatcacgct aatactttta
    10981 gtcttaagtg gactcagaag aacaaataca gtgaatatta ttattgtaat gattacattg
    11041 ataactttaa ccgtttttat aataagcgga ttacctgatt tcatgcaaag gggaactgct
    11101 aatctgaggc ccttctttcc tgtttcaaaa caagaaggat taagtcattt tttttatgct
    11161 actgctttaa tgtttgtggc atataccggt tatgggagaa ttgcaacatt agcggaagag
    11221 gttaaaaatc caggaaaatt tattcctgca gcgattatta tcactctgat ggtgagcgcc
    11281 gtactttata ttctggtggg tcttgttgct atcggagctg taggggttga aaaactggcc
    11341 caagtaactg aaacaaacgc aaccccttta gagagcgcgg cacaagccat tggtatcccc
    11401 ggattgagta ttctggtctc cattggggca tgcacggcca tgttaggggt attactcaat
    11461 ttaatacttg ggttatctcg tgtggcttta gctatgggta gaaaaggaga tttaccgaag
    11521 atgttttgct atgtatcaga aacaaggcat gttcctgttt ctgctgtcat tggagttgga
    11581 gttgtaatta ttgctctggt attgactgga agtgttgaaa caacatggac attcagtgcg
    11641 tttacagttt taatctatta cgccattact aacctcgctg ccttgcgttt gcctaaagag
    11701 gagcaattgt atccacctct gtttgctatc ggaggcttac tgtcctgcct tttcctggca
    11761 ttttgggttc cgattggagt atggataacg ggaatcagtt tacttgtcct ggggcttctt
    11821 tggaaagttg ttgccaatcg ttttttataa ggtaaaaata tagacaaatt tattcaaaat
    11881 aaataagagg gttagaggaa aggaacagca atgatcggtg agattagggt taattttaca
    11941 gttgctgtct ttggctttta ttgataattg ttttgatggc ggtttttttg ttccattgaa
    12001 gggaaaacta aggggttatc aaacttgaac aatgttggac taaaataatc caacattgtt
    12061 ttcgtcttat tgcatggttc ttgatattgc ttttccagtt gctttaatat cttgtttagc
    12121 tcctgttaca gtccccttga cagtatggca tgctgctaat agagcaatgc tgatactgag
    12181 agtccctaac ttgattaaat tcctttttaa gttaaacata acccctcctt tatgcttatg
    12241 attcacttta attttaaatg cccgcttttt tacttgcctt tgcatgtctt ggacaagtgg
    12301 ggcgcgcagg tcgttaaatg agccatataa aaatcaaata attacgttgg tatttttagt
    12361 taattttcat aaaaagaatt ctttacataa agaatagaag agtattctta attttcaaga
    12421 tacaatttct atccaatgaa acagccagga tttacgaaaa aagtaatcgc cagaatttgc
    12481 tcttgttaca aaattatttg taggtaataa aactgatacg ccccgttcgc tccaaacgag
    12541 cttctttcac tgttgtcaaa tcgcttgcat ttaatttttc tctgaaaact tgcctcaaat
    12601 cttcttcagt cacttgatgt cttttcaaag cactccaaat gagctgtcca tcttgtatta
    12661 atagcaccga tttcccttta accagttttc caaatccgtg ggaataaaag gagcaaatag
    12721 caaagaccca atgtaaaaaa ataagtaaac tgctcccagc cattgcagat attaaagttg
    12781 agttgccatt aattgcacga cttagcacgg cgccaatgac aaatacaaaa atataatcaa
    12841 atgctgtttc aaaattgtat cgggtattgc caacacggat gataaaaatt gcgaaaacat
    12901 agataataaa agttcttata aaaagatata aaa
//
LOCUS       DATNSN010000099        12595 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.099, whole genome shotgun sequence.
ACCESSION   DATNSN010000099 DATNSN010000000
VERSION     DATNSN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.099"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            306..7255
                     /locus_tag="VAH76_002139"
                     /pseudo
     CDS             306..7255
                     /locus_tag="VAH76_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
     gene            7252..9282
                     /locus_tag="VAH76_002140"
     CDS             7252..9282
                     /locus_tag="VAH76_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386746.1"
                     /translation="MRSQTIRLTQKDFPIFGKAFSLVNISFLVINENMELEYINDTAK
                     KKLKINWSSTDRKSFFQLWELANLTPILTKKGDLIPNKILQVGSYSIQWQKTVAIVNE
                     KHYILLMDKDVATTSHLFEEIGAAIYGEIGYAPKNKISVKEYIQELTNYYTSVIDKIP
                     CYVYWKNTSFEYMGCNEMAAKFFGFKSTSDIIGKNDFDLFQDPDFAKNYQEQDKQVFS
                     TGEPILKMPSDLKDRNGMVTNTLVSKVPITNLSGSIVGLVGITVDVTELTKAKEAAEA
                     ANQAKTAFIANMSHDIRTPLTGVIGMSELLENTVKDPEQKEEAHMLHDSGEELLNMLN
                     DILDDVHAGFMDKEDIRIETFDLYQCIEDLVKLERPTTVMKHLELIVHIGKSVPRYIQ
                     SDRKKIHRVLLNLLGNAIKFTPKGQITIQVACLEVSGKNIHLQFKVSDTGIGIPTSLQ
                     EKVFERFFRISPSYKGIYKGHGLGLHIAQSYVNLLGGHIALISEEGKGTTFYFDIPCQ
                     TGTQKKIKTNKLTNSSKKIDSSLSTSSNSQLTPKLSSKTEENSPYVLLVEDNNIALKV
                     LELILSNEGYRFKSASNGEEALALIQTESFDLVITDLGLPGISGCELSVKIREWEKKH
                     SKKPLPVIGLTGHTKDSSEKGCLASGMNNVFTKPVTPELIRNIINKYLLGEK"
     gene            9513..10421
                     /locus_tag="VAH76_002141"
     CDS             9513..10421
                     /locus_tag="VAH76_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386747.1"
                     /translation="MSQQIGNEFFEADMDMHQHYLDILNCVPDVVYWVDKDCHLKGCN
                     QNFIKLLGLKKIGDLKGTPYDQMAKHMKWSEKRIETFKLDDMKVLFSGESQYDIEEEP
                     VYNKGKEAIYYKSRRVPLFDKENKVCGLVVVLTDITDLKKLEAHIQQTHSPRNIKRTQ
                     KKRVTEPKILMVEDNFVAQKVEEALLKELQCQVDIADTGDKALNLFDPGKYDMVFMDI
                     GLEDTSGYLVAKKIRQKEQNTNYHVPIIALTSYQADVVKYDVEDYFMDGVLTKPLTSE
                     QADQLIKHFVHKEDIMVSGLKSAREN"
     gene            10703..11464
                     /locus_tag="VAH76_002142"
     CDS             10703..11464
                     /locus_tag="VAH76_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386748.1"
                     /translation="MIWDPERYVLGNYIQNKINDVFLKTIHSKLSGNILDIGCGDGHY
                     TSLLAGKVKHGQVLGIDSSEQMIMHANQQWARTGLSFEVHNIEEFHQPQSFDLALSFW
                     CLHWTNIHISFPNIFHLLKREGKLYAVMSSFSDHSILQTWKELAKQNLYRDLTEQYIS
                     PINEQYFYGVINLLNRLPFKRIKLDLKTCRVHLPHIDYYKNLLLTMPFIKRVPSEITD
                     TLVEDMLKAFQTICDRKYGGQLYYETRPIFLEAIK"
     gene            11489..12523
                     /locus_tag="VAH76_002143"
     CDS             11489..12523
                     /locus_tag="VAH76_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEO1386749.1"
                     /translation="MHLNFNTTPGMNIPMVIANQDNLKASPTPLLHFISEYHDEIIQK
                     LNQYGAIVLRGFSCQEENCFSKAIELCALGTRCSTSDYDLPRTVLANEIYTSSDLPAH
                     IPLPLHHEKPRSPKPPNHIYFCCIIPPQEGGGTIFANAEEIWIDMPQDIQNKILEYGV
                     QYKQFFHGQSVKYRVLRKILGNHCARSWVDYFGTEDKTQIEQNLTQKQVVWDWINHGN
                     DLIILNYLPGALKHPLTDKIAWFNSSAYLNYYSNLNYGELKNLRSFKYWASRYLILKD
                     MLPMICHYGNGQEFSAKEISEINQVIQRHTRVFHWQKGDFMIVDNFTFMHGKQAHVGE
                     RLLYSCMTAY"
BASE COUNT         4228 a         2393 c         2307 g         3667 t
ORIGIN      
        1 aaataaaaac tatttataca acagaaagct ccaatcaaaa aagattatga tttcattttc
       61 ttttttcttt agaggaggct attgcttatg catctctatc tattgcctag taatcacccc
      121 aaatttattg tattgataat ccctctgaga caaatgaatg aaagaatgca tgtaactgtc
      181 tctttaaact attccatcat tcatctgttt agttgtcttt tttttgtgta ttttttgaac
      241 catagacacc ttttttgaaa aactgcgata taatttcacc aaatttttaa ttaaggcctc
      301 gaaatatgga attaaaaaag caggatttta attatacagt cattgacctt tttgaagcac
      361 aagtcaaaaa aaatccagat cttcttgctc ttaaagacaa gacaacagcg tacagctacc
      421 tcgcattaaa tgaaaaagca aatcaatttg ctcgaagttt gaaaaaaaaa tcagttaaag
      481 aaggcgattt tgttgccatt ttattagatc cgggcgttga attttttatt tgtatccttg
      541 ctatcataaa acttggtgcc atttatgtac ctctcgatac tcttgcacca caaacacgtt
      601 tgcatgaaat cgttaaagat gctaatcctg gtattgtgat tacccatgat gaataccaac
      661 ctctcctcga aggcattgat actgaaattt atctaatcaa acaacttcat ttggagtcat
      721 taagtcaatc caagaaaaat attaaaaaca cggtcatgcc aacatgccct ttgtatatga
      781 tttatacttc tggatccacc ggaaaaccca aaggagtggt tgttcctaac agagcggtgg
      841 taaatcttgc cataattgat aatatcatcg cgatacaaaa aggcgaaaaa gcaggccaat
      901 tctgcaacct ggctttcgat ggcagtgtat tcgaaacatg ggttgccctt ttaaacggcg
      961 cagcattgca tattatcccc aatgaagcca gaagaaacca taatcaatta aaaaagatta
     1021 ttaaagaatt ccaaatcaag tacttactga ttccaaccgg ctatttccat cagctgatga
     1081 aatcatttcc agaaacctta gatcttttac acactatttc cttggcggtg agcaagtgaa
     1141 tgttcatctg ttaaaaaatt ttattgatta tagaaaaaaa gcagatatcc ccgcaaaact
     1201 ggttaacgta tatggcccaa cagaggctac cgtattctca agttgcaata ttattcatag
     1261 cgaaaaagaa tatacagatg agcaactcgc aagtataggc tttaaaacag ccaacacaaa
     1321 aacctatatt cttgatgata accgccgtcg cacagaggaa ggagaacttt gggtcagtgg
     1381 ggtacacttg gctctgcatt accataacag cccggaacaa acagaagaaa aatttgttaa
     1441 gaatcctttt tcacgcaacg aactctattc aagaatgtat agaaccggcg ataaagttcg
     1501 tcagttacct tcaggtgaat tgttatactt gggtcgcgtt gatgatcagg taaaaattgg
     1561 cggcttcagg atccatttaa ctgaaatcga acaagagctc atgaatcata aggccataag
     1621 ccttgctact gtaaccgtag aaattggtgg tggagcacac aaatttctta cagcatacct
     1681 ggtttttaaa tcaaaggaca ccattgttga tgctgacgag attagaaatt tcctgagcca
     1741 aaaactacca ccttatatgt tacccgccaa atatgtcatg gttgaaaaat tacctctaac
     1801 cgcagtgggt aaaattgata aagccaaatt agataaaata gctcataccg atttatcatt
     1861 tcatatcgac acatcatctt caaactctat tgaagaaaca ataaaaaata tctggcgaca
     1921 ccttttaaac cgttccagta ttgataccca caagaatctg ttcgaattag gagcaaattc
     1981 attactcatt acagaagcat gcgctcaaat taataaagaa ctccagtgtg atttacagat
     2041 agttgatatg ctgacccacc cgactatcca caaattaagt cgctttctcg aaggcgattt
     2101 ggatacacat cttgcacgaa aaataaaaaa agccgcttca caggacattg caattattgg
     2161 aatgagttgc cgctttccca aggctagaaa tatagatgaa ttctggcatc atttgtgtca
     2221 ggggaaagat tgtcttactc gttttgacga aaatactatc ccccgctatc ttttggccca
     2281 agaagactat gtgcctgtaa aaggaatctt ggaaaatagc gatcattttg acgcgcattt
     2341 ttttggcttc agccccattg atgccaaaat taccgacccg caacatcgtg tctttcttga
     2401 gtgcgtatgg gaggctcttg agcatgccgg tattgcccca ggcaaactgg actctaaaat
     2461 catcagtgtc ttctcgggaa tgaccgacag cacgtatctc aatgaaaatt tacttaaaaa
     2521 ccaatggttt tgtaatgagt ttgaccaatt tcagcaaaga atagctacaa gcatgggaat
     2581 gcttagtacc caaacttcct atcggctcaa tttcaaaggc cgtagtgtta atattaatac
     2641 cgcctgctct acaggtttgg ctacggtaga tcatgcctgt caagacttaa tactgggaca
     2701 aagtgacata gcgctggctg gtgcaagctc catagtcgtt cctgaaacac ggggatattt
     2761 ttttcaaaat ggcagtattg tctcacccga cggatactgc aggccttttt catctgaagc
     2821 aaacggcact gttttttcca atggtgttgg tgtcattgtt ctaaaacggt tagaggatgc
     2881 aataaagaat cgcgatacaa tctatgccat catcaaaggg agaggagtaa ataacgatgg
     2941 aatagacaag ttgggtttca ctgcaccgag tatgagtggc caaatctcat gcattcggga
     3001 tgctcttcaa caggcccaga ttagtgcaga agaaatcagt tttcttgaag cacacggaac
     3061 agcaacagca ttaggtgatg ttattgaaat acaagcatta acttctgtct atcgagaaca
     3121 aactgataaa acccagtttt gtgctttagg ctcagtcaaa gcaaatattg gacatactga
     3181 tgtaacagcg ggtattgctg gcattataaa aacagctcta tgcctttatc ataagaaaat
     3241 cccacctctt attcattata aaaaaccaaa ccccgattta tcactgcccg aaagtccctt
     3301 tttcgtcaat acggaaactc aagattggga cgctgcttca aaacgctatg ccgcagtaag
     3361 ttcttttggg ataggcggca ccaacaccca tatgatttta tccgaacatg aacatgagga
     3421 aaaaatggct tctgttgccc ccttgaaaga agaattaata cttctttcag caaaaacaga
     3481 aaaagcttta caacaaagta cttacaacct caagcactac ttaaaaaaat gtgatgactc
     3541 caatcttggg aatgtcgcct atacgctcta tagaggtaga gaagacttcc aatggcgcca
     3601 atttgctgtt ggtaaaacag tggaagagat atgtgccgag ctcaaaggaa aaaaaccttt
     3661 attttttaat caagatactc accatagtat cgcatttatg tttagtgggc agggaatgca
     3721 atatccccaa atggccatgg aattatttaa aacaattcca ttatttgcaa aatacgtgac
     3781 ttcaggggca gagattgcac aatcctattt acattgtgat ttatttccaa tcctccatga
     3841 ttccagctca aagaaactta aagagactca atacgctcag ccggctctat ttattgtgga
     3901 atatgctttg gcaaaattac ttatggagtg tggcataata ccagagatgc taatcggtca
     3961 tagcattggc gaatacgttg cagcgtgcct tgccggggtt ttttcttttg aagatggaat
     4021 cgcattggtg tgtgagcgag gattattaat gtcaaggaca cctaaaggtg ccatgctgtc
     4081 tctcgatgca acacaagaac aactggatag ctacctgaaa atggttgatg ttgatatcgc
     4141 cttacataat gccaccaatc attttgtttt atcgggcagt gaacccgaga ttgagaaatt
     4201 agaaagctac ctgactgctc ataaaaagcc atttcaaaaa ttgaaagtaa accacgcctt
     4261 ccatagccgt tttatggaat ccattgaaat gccttttaaa gaaatgttta ctaacataaa
     4321 actctcggca ccccaaatac cgattatttc caatgtaact ggagattggc tctcagcagg
     4381 tgaagcgatt aatccggatt attggtatag acacctgcgc gaaaccgtac aattttgcca
     4441 gggaatcaat cgcatactgc aagataaaca tccaattttt atcgaagtag gccctggaca
     4501 aagcttgtat ggatttgtgg gggaaatatc acataaaaag gcaaatgctc tttttacact
     4561 ccctggttgt caaaagagac tatctgacag gattcagtta ctctcagcat gcggtgaact
     4621 atgggcaaaa ggggttaaca ttcgtttaga atccctggta gacaatgaca accgaaaatt
     4681 tattcccttg cctacttacc cttttcaaag acaaagatat tgggtcgatc cggataaaaa
     4741 aaacatttct gtcaatgaag cctgctcgtt atataaaccc gtatggtctc atgaaaaaat
     4801 agctcatgtt actcctgcta atcttaaaaa ttttgactat gtcattttca aagataaaac
     4861 aggaataggt cagaagataa ttaatatact aaaaaatcat gacatcgaac ctattgttgt
     4921 tgaattaagt gaggaattca gacaattatc gaaaactcat atcagtatca atccaggaga
     4981 aaaatcagat tacaatcaat tcgtttcatc gctaaaaaat acgctgcgtc atccaattat
     5041 ttttcattgc ttttcatgtg atagctctga ggatagcttt cttgatacac agcagattga
     5101 aaaccgatta aagcttgggt tttatagcct cctctatctg actcaggcat tcttaaatga
     5161 tttgggaaca aacattccaa ttcgcttggc aattattact tctggcactc aaaatgttat
     5221 tggtactgaa aagatatctc caattaatgc cagcctggtt ggcgcttcgc gcgtcattcc
     5281 acaggaacac cataatattt catgtgaact gttcgacatt aatttgtatg aaacaaccca
     5341 aaatattacc aatgcgatta tcgacattgt cagttcttct acgcaagcag cacaagatct
     5401 cccctaccaa ttaactgcct atagaaatag ttatcgctgg gaactctctt attccccaaa
     5461 tcacaacaag actgttttac aacgtttaac agataatggc atctatataa tcacaggtgg
     5521 cttagggggt attgggctat cactgtgcca tagcatcttg aagaatacca aatccccaac
     5581 atttattctt tgctcaagaa cacctgtgat cgatgaatca gactgggaaa aaatagccac
     5641 agatacttct catccccatc accataaggt attacaacta aaaaaattaa aagaacttgg
     5701 agcaactatc gtttgctatc aagcccatat tggaaatgat aaggatgtcg atcgttttat
     5761 cacattttgt aaatccagat ttaaaacgat taacggtctc attcatgctg caggagttgc
     5821 tggtggcggt ttgttacaac tgaaaacaaa agaaaacgcc gataatattt tgttaccaaa
     5881 aattcatggc acctatcact tggcaaaagc attacgcgag ttccatcttg attttgttgt
     5941 tttcatgtcc tcaatcgcat cgattactgg agagagagga caaattgatt attgtgcagc
     6001 aaatgcctgc ctggatactt ttgcaaacag caatctattt tatacacaat gtttgcttag
     6061 tattaactgg aacacctggc gtgatgtagg gatgagtgta gaaacccaaa agccggaaga
     6121 tatcgatttt tttgctcggg gaaatactat tagtccatta caaggacaag agttattttt
     6181 aaaagcactc tctagcaatt gctccaatct cattgtgtcg aattataata ttgaagacta
     6241 ttccagcttg atacttgata ataatcctgc tgatacagaa gaacataaag tttcccgcga
     6301 cacatttagt atgagtcacg aattttgtgc cccacaggga agcattgaaa agcgtcttgc
     6361 aaaattatgg caagacaatc ttcatattgt cgatattggg agaaacgatg atttttttgc
     6421 attaggaggc cattcgttaa aagcattaca cctcattgag aacattaatt ctattctagg
     6481 ggtcaattta tccattcaac atttatacca agcgccaacc attgccaagc ttgctgaatt
     6541 aatctattca aaaaaccccg aaaatcagat tgaaattgta atacctctga aagaaacgag
     6601 ctcaaagcaa catccttttt tcttctgcca tcctgcaagt ggcatggttt attgttttga
     6661 tgatttggca tccttttggg actacccaat ctctttatat ggtcttcaag atccaagtgt
     6721 ttcctctgga aaattaattt ttgagagtct ttcctctatg gcaaaggcat atacccaagc
     6781 cataaaaaaa gtacaaccac atggtccata ctttttggta ggatattcgt ttggaggaag
     6841 cttgatgcat gaggtggctc atctactcac tcaacaaaat gagcgcattg gattacttgc
     6901 catgattgat agctggtgta ctttctcaag caagcaaaaa tccaaaaatc atttcattga
     6961 ggcctatcta tcaaaagaat tgcaggctag cagaaatttg gctgatttgg catgggaaag
     7021 aatggaattg ctactcaatc ataagccaac aaaaatcaac caagacatgg ttctatttaa
     7081 agcaacagat ttaaatcaag actatttaga tatcgatgat ccaatgaatg gatggggaca
     7141 atataactta gggcaaatta tctgccatac tatagaagcg aatcacgata caatcattcg
     7201 tcagggatat caatttattg cgcaaaagtt aattgagtac agggacattg aatgagatca
     7261 caaaccattc gtttgactca gaaagatttt cccatttttg gaaaagcatt ttcattagtt
     7321 aacatttcat ttctcgtaat caacgagaat atggaattgg aatatattaa tgatactgca
     7381 aagaaaaaac ttaaaattaa ttggagctca accgatagaa aatctttttt ccaattatgg
     7441 gaattagcca atctaactcc aatcctgact aaaaaaggcg atcttattcc taacaaaatt
     7501 ttacaagtgg ggagttattc tattcaatgg caaaaaacag tcgcaatagt caacgaaaaa
     7561 cattacattt tgttaatgga caaagatgtt gcaacaacct cacatctttt cgaagaaatc
     7621 ggggctgcta tctatggtga aataggatac gcacctaaaa acaaaatttc tgtaaaagaa
     7681 tatattcaag aattaaccaa ctattacacc tccgttattg ataaaattcc ttgttatgtt
     7741 tactggaaaa acaccagttt tgaatacatg ggttgcaatg aaatggcagc gaagtttttt
     7801 ggctttaaat caacatcaga tattattgga aaaaatgatt ttgatctttt tcaagatcct
     7861 gatttcgcta aaaattatca ggagcaagat aaacaagtat tttcaaccgg cgaacctatt
     7921 ttaaaaatgc catctgatct caaagaccgc aatggaatgg tcactaatac attagtcagc
     7981 aaagtaccca taactaattt atcgggaagc atagtagggc ttgtgggaat tactgttgat
     8041 gtaactgaat tgacaaaagc aaaagaagct gctgaagcag ctaaccaagc caaaactgct
     8101 tttattgcca atatgagtca tgacatccgc acaccactta caggggttat tggaatgtct
     8161 gaattattgg aaaatacagt gaaagaccca gagcaaaagg aagaagcaca catgcttcac
     8221 gatagcggtg aagaactatt aaatatgtta aacgatattc tggatgatgt tcatgctggc
     8281 tttatggata aagaagatat ccgcatagaa acttttgatt tgtatcaatg tattgaagat
     8341 ctggtgaaac tcgaacgccc tactacagta atgaaacatt tggaattaat tgtgcatatt
     8401 ggtaaatctg tacctcgtta tatccagagt gatcgtaaaa aaattcatcg agttctttta
     8461 aatttattgg gcaatgcgat taaatttacc cctaaaggtc agattacgat ccaagtagca
     8521 tgccttgaag tttcaggaaa aaacattcat ttgcaattta aagtcagcga tacggggata
     8581 ggtattccaa ccagtcttca agaaaaagtg tttgagcgtt ttttccgaat cagtccctct
     8641 tataaaggga tttataaagg acatggtctt ggcttacata tcgcacaatc gtatgtcaac
     8701 ttattagggg gccatattgc tttaataagc gaagaaggca aaggaaccac tttttatttc
     8761 gatattccat gccaaacagg cacgcagaaa aaaataaaaa ccaataagtt aacgaacagt
     8821 tcaaaaaaga ttgactcctc gctctccacc tcatctaact cccaattaac tcccaagttg
     8881 tcatccaaaa ctgaagaaaa ttcgccctac gtattgttag tggaagacaa caacattgca
     8941 ttgaaagtgc tggagcttat tttatcaaat gaaggttatc gctttaaatc tgcttctaat
     9001 ggagaagaag cattggcatt aattcaaacc gaatcgtttg accttgtcat cacagatctt
     9061 ggccttccag gaatatctgg ttgtgaactt tctgttaaaa ttcgtgaatg ggaaaaaaaa
     9121 cattccaaaa aacctcttcc tgttattgga ttgactggac atactaaaga ttcctctgaa
     9181 aaaggatgcc tcgcgtctgg tatgaataat gtgttcacta aacctgtaac tccagagcta
     9241 attcgcaata taatcaataa gtatctatta ggagaaaaat aaaagaaaaa ctcggttaat
     9301 ttaaaattca cttgattcat ataataaata agcaaactac ccaagcgaac ccttctaata
     9361 tagcgaatta attaaagaga caattggcaa atcacaattg caatttacac tgccctttat
     9421 ggacaattaa acaacattca ggtctaattt ttgctataat tattttaacg tagctccaaa
     9481 agagtttaag tataatcttc aggaggaaat caatgtcgca acaaattgga aatgaatttt
     9541 ttgaagcaga tatggacatg caccaacatt accttgatat cctaaattgc gtaccggatg
     9601 ttgtttactg ggtagataaa gattgtcacc tgaaaggttg taatcaaaat tttatcaagt
     9661 tattgggctt aaaaaaaata ggcgatttaa aaggaacccc ctatgaccaa atggcaaaac
     9721 atatgaagtg gtcagaaaaa cgaattgaaa cgtttaaact ggatgatatg aaagtactct
     9781 tttcaggtga atctcagtac gatatagaag aagaaccagt atacaataaa ggtaaggaag
     9841 caatttacta taagtcccgt cgagtaccat tgttcgataa ggaaaataaa gtgtgtggtt
     9901 tagtggttgt gttgactgat atcaccgatc taaaaaaact agaggcacat atacaacaaa
     9961 ctcattctcc caggaatatc aaacgcactc aaaagaaacg agtgactgaa cccaaaatat
    10021 taatggtcga agacaatttt gttgcacaaa aagttgaaga ggcattgctt aaggaactgc
    10081 aatgccaagt tgatatcgcg gacacgggag ataaagcatt aaatcttttc gacccgggga
    10141 aatatgatat ggtctttatg gatattggtc tcgaagatac ttctggctat ttggtagcaa
    10201 aaaaaattcg ccaaaaggaa caaaacacca attatcatgt gccaattatt gccttaacat
    10261 cttaccaggc tgatgtggtg aaatacgatg ttgaggatta ttttatggat ggagtgctca
    10321 ctaaaccttt aaccagcgaa caagcagacc agttaatcaa acactttgtt cataaagaag
    10381 atattatggt ttcaggctta aaatcagcaa gagaaaatta agatcaactt gcttagcaga
    10441 caatatctga tttttagttt gttcgcatga aacaaaatga aacattgcca aaacctggta
    10501 aatagagaaa gggttaaatt agcacatccg tgcactacag gattaaagta caacctcgtc
    10561 ggcgaggact gtatcctcat aaaaacaaga cacatcccgc ataatgtgtg agaatagatt
    10621 tcagccatgc caaagatatt tgtaatcgct ggtttcttgg gtatatcttc ccttacaatg
    10681 attattgata ctggagaatg aagtgatatg ggatcctgaa cgttatgtgc ttggaaacta
    10741 tattcaaaat aaaatcaacg atgtatttct aaaaacaatt cacagcaagt tgtctggaaa
    10801 tattttagat attggttgcg gagatgggca ctataccagc ttgttggctg gcaaggtaaa
    10861 acatggccaa gttctgggaa ttgatagctc tgaacaaatg ataatgcacg ccaatcaaca
    10921 atgggcacgt acggggctat cttttgaagt tcataacatt gaggaatttc atcaaccgca
    10981 atcttttgat ttggcgctct cattttggtg tctgcattgg acaaatatcc acatttcttt
    11041 tccaaatatt tttcatcttc taaaaaggga aggcaaattg tatgctgtta tgtcttcctt
    11101 ttccgatcac agcattttgc aaacctggaa agaattggcc aaacaaaacc tgtataggga
    11161 tttaacagaa caatacatct ctccaatcaa tgaacagtat ttttatggtg tgattaatct
    11221 tctcaacaga ttgcctttta aacgaattaa acttgattta aaaacttgcc gtgttcattt
    11281 gccgcacatt gattattata agaatttact tctaaccatg cctttcataa aaagagttcc
    11341 ttctgaaata acagatacac tggttgagga catgttaaaa gcatttcaaa ctatctgtga
    11401 caggaaatac ggtggtcaat tatattatga aactcgcccc atatttttag aggctatcaa
    11461 ataactcaag gtgttaggag gcaataccgt gcatttaaat tttaatacaa ctcctggcat
    11521 gaacatacct atggtcattg caaaccagga taatttgaaa gcaagcccta cccctttgtt
    11581 acattttatt tctgagtatc atgatgagat aatccaaaag ctcaatcagt atggagcaat
    11641 agtattaaga ggattttcct gtcaagaaga gaactgcttt tcgaaagcaa tcgaattgtg
    11701 tgccttaggc acgcgatgtt cgacaagcga ttatgacttg ccaagaactg tactggcaaa
    11761 tgaaatttac acatcaagtg acctgccggc tcatattccc ttaccgctgc atcacgaaaa
    11821 accccgatca ccaaaaccac ctaatcacat ctatttttgc tgtatcatcc cgcctcagga
    11881 aggcggtgga acaatctttg ccaacgctga agaaatatgg atagatatgc cccaagacat
    11941 tcagaacaag attcttgaat acggcgtcca atacaagcaa ttttttcatg ggcaatctgt
    12001 aaaatatcgt gtattaagaa aaatcctggg aaaccattgt gctcgaagtt gggttgatta
    12061 ttttggcaca gaagacaaaa ctcaaataga acaaaattta actcaaaagc aagtagtatg
    12121 ggattggata aatcatggga atgatttgat aatactaaat tatttgcccg gagcactaaa
    12181 gcatcctcta actgataaaa tagcctggtt taattcatca gcttatttga attattattc
    12241 caatttgaat tatggtgaat taaaaaacct gcgttcattc aaatattggg caagccggta
    12301 tcttatcctg aaagatatgc ttcccatgat ctgtcattat ggtaatggcc aagaattttc
    12361 tgcaaaagaa atatctgaaa ttaaccaggt tattcagcgt catactcgtg tttttcattg
    12421 gcaaaaagga gatttcatga ttgtggataa ttttactttc atgcatggca aacaagctca
    12481 tgttggagag agattattgt attcatgcat gacggcttat taatgctgga ttatttttat
    12541 ccgctccctg acagtcgcgg ttcagtgttt gaaccataat ccgagccgcg actgt
//
LOCUS       DATNSN010000100        12425 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.100, whole genome shotgun sequence.
ACCESSION   DATNSN010000100 DATNSN010000000
VERSION     DATNSN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.100"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            185..11530
                     /locus_tag="VAH76_002144"
     CDS             185..11530
                     /locus_tag="VAH76_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1386750.1"
                     /translation="MVNVTMKNKTKDLLNEPIAIIGMNCQFPGIDSDIEDVSAFYDML
                     MKGQTPIKDVPENRWNIEEYYSADRKKADKIISKKGGFLDNTHLFDAAYFKISSAEAK
                     QIDPQQRLFLEVAIRALNDANIPLDSLKDSNSGVYCGISTHEYSQLNYKDHIKFNAYT
                     PIGIANSAAAGRLCHFLNLKGPSMAVDTACSSSFTALYLAATALRNQQCDMAIVGGVH
                     LSLCPESFIGLTKANMLSATGQCSSFDSKADGFVRSEGCGVVIVKRLSDAIRDNNKIY
                     ALIKSMVINQNGDGTGLAAPSTNAQIAMHQAALEHAHLTAGEIDYIETHGTGTTVGDP
                     VEFNAIQSIHQGHHSAQKPLIVGALKSNIGHTISSSGIASLIKVLCSLQNEAIPPNLH
                     YSNPNNLIDPESIPALLPVEAIPFPKLKNKKRYIQVSNFSFTGTNVSVILEEAPELES
                     HESKQDNNEPKCFVASANSEFSLKQLMSRYEHYLKNSSSTLGDICHTLINCRDHYKFR
                     CAIIVTDKKELIKKIESKDYEIKKVVLQNNPRTVLNDAKAIYDAYLSGINIITDDENT
                     AYNKVDLPLYCFDRKPYWHEVRKQVKNDHWLDSLYQQPMAQQVDAIKAEIASQIKDLL
                     KKDSIDPHQDLETLGLTTSLLESLDQILQDMFSPRYKVPSLQSLRYLTLDKLARHLQK
                     MIMPPVVYRQPSINVLNVEPIAIIGMSCRFPKAANVDEFLSLLEKGESGMIDIPLERW
                     DNEKFYDSDVDALGKLYIKQLGFIDHVNHFDAEFFNISPREAKLMSPQLRVFMETSYH
                     ALEDANLSLDAVKDTNTGVFVGVGTNEYPRVLAYQGVTLEDLNIYFATGNVLNALAGR
                     VAYSFDFHGPIQAIDTACSSSMTAIHNACLSLQSGDCNMALAGGVNIILSPDSNITLS
                     KARMLSPDSRCKTFSEDADGYGRSEGCGVVVLKRLSDAVKDNDNILAVIKGTSINSDG
                     KSGGFTVPNGAAQEEVILSALAKANLTPGDIDFVEAHGTGTPLADPIEVNTLTKIFSE
                     HHNKENPLYISSVKTNIGHSESASGVASVIKTVLSLKTKTIFKHLNFKKLNPAIQLIN
                     TVIPLDKTDWAKKQGLRCAGVSSFGFSGANAHAIIQEAPEDKKEARALPEESLLVLSA
                     KSKTSLELLLASYQNYLSNTREEFADICYTAATCRTHFMYRVAIRAQNATQAANIIKN
                     NGYTICHITKEKEWAQQPDTLAELQTAYLDGFLINWSEFYRSFGHHFAKVKLPLYEFE
                     RIEHWFGDKNKLKDAPLPKDWCFQIQWQNEPCDKSNRKLRGNRWLLIGPRHLADGFKA
                     QGLEIIYEDEEYSRDKLEGIIFAESLHLAPPDIEANIDFQKKTLKKLLNLIKELDNQS
                     IDLQLLILTSNGIAELANDKHPVNISNSPLIGFCKTLALELPQFKTILIDMDKIEEDK
                     YPTQVIEEINYNHGSRYEHIVAYRGGERLASRLKKTPLEDRRRLLHGVKGRYLITGGC
                     GGLGLVTAQSLLSSGARELILTSRNIDKPRIKEAIYKIKSHYPGANIRVVSLDITDRE
                     SLKNLLSELNTDGQLKGIIHAAGAAIKAPLLEHQDEDVDYLFSAKVKGGWYLHELSLD
                     CNLDFFVVYSSVSSVFGSNKESVYSGTNSFLDALIAERQRMGLPGTAVQWGPWGEVGM
                     AQKRSRDEGLKQALISNAQGHVIIKCLINDELKHATIISPEYLKFMLDFVPKPLPGFY
                     GQLDEELEKIQQQHHQSPAKNLSPWLNQYLEISEEERFKACKAMVSDICTGILELTEN
                     EDLNEDEGFFELGFDSLMITELASELKKKLEPFLKVTVTIGFNYPSISKLAKYIESEL
                     DKNLVKIQSPKSAPKPIDDGIAIIGMSCLLPNAPDIAAFEKLLEEGLSGIKDIPNDRW
                     DNNQYYDPDMEAPGKSYVTKLGLIENIKYFDAAFFGISPREAKLMEPQQRVFLECCYK
                     ALENANYPSESLRGSLTGVFAGVGPNEYYAQLEKSGFSNEELSAYSITGNVLNLIPGR
                     VAYTFDFKGPSISVDTACSSSLVAIHYACQSLKNKEIDYALAGGVNILLMPESNVTLC
                     KAKALSPDGQCKTFDERADGYVRGEGCGVVLLKRLSDALRDNDHILAVIKGSAVNNDG
                     KTAGLTVPNGKSQEEVMKKALGQTELSSTDISYIEAHGTGTPLGDPIEVDAINKVYGH
                     QRNKENPLYLGTVKTNIGHLESASGVAGVIKTVISLQRKTIYKNLNFNKLNPNIKLDG
                     TQLALEKTNWNTNTKLKYAAVNAFGFSGTNAHVILAEFPKEEKPKTSKPSQKHLLVIS
                     AHSKTALDNLTQRYQQYLETTEHHFGDICFTAATCREHYHYRLALTAENAVEASRMLG
                     AGQFALSYHENITLDLQNDRALNSLLMDYLQGKPVDWKSYYQSLKGDFTKVLLPNYTF
                     DRSEYWLDKKNNDSMATHKDEQHPLLGQMLAMPGNEYLFHQKLDLENLAYIKQHSVFE
                     KVIFPATAYIESGLAAAKSIFQSSVFQIEKFNIERPLHPKQGQEFQLQVKPKMDGQYK
                     INLFAKQENDWQTFCEMEIHAKPTAARESIHIDVLKSSFGDRVELSDIYEQFQKSSLF
                     YGDEFHVLQEGYVQANSVLAKVTLTKASQGQDYYYHPVLLDGAMQSILLLSVKNDENK
                     NFNAENITYIPYAFTRMTVFQEAPRSLWVHLTKQNTEDASDLCVNMNLYEQSGLLIAQ
                     IAGLKLRRVTRSHFVSYDAALSHLYYTEWSPVPFKTLPHQQEIPDLLVITKNPGKAKE
                     ILDGLNYHLVERIDDCTNIENSNIVFLYEQGQFHDLFHCCQILFRLRPKRFILVTEHA
                     YAIQDLDQVNPYHTMACAFWKSFRNEYEPNKNYAIDLGSRSRLAKALMYLLNADTHDT
                     QIAVRESLYLPRLKKKQLVSNPDPERLIDGNATYLITGGTGGLAKPLMEYLMRRGARH
                     IAITSRTHYPDDINALIDKAKQNKIDIKHYMVDAGNYQKMEQVIGEIQQSPNPLKGVF
                     HLAGLIQDGLIVNLNDEAMQKVISAKMDGALILHQLTQNIPLEWLVMFSSSASLLGAR
                     GQANYAAANGFLDGLAHLRRQQGLPAISINWGPFHTTGMAANLTHTLQHYGFLLLDKD
                     NIDILDVLLPSQLPQISPCPMNWDVYCKHTPKHMELSELVKAKPLPDQSFLNILRQHS
                     KEESISILSQALREIAADVLALDDSEHISNDHDLFSMGMDSLMSMDIRNRIYDKLQCQ
                     TLSLPIEYFINTPTINKIARHISDELDHVFDSHSDYRPTENPPEREIALCDFQYVFWV
                     LNKLDYHFNIGTQIQLHGRLNKDYVQRAFEFVVNQNSVFWIHFNKDKPTQKLHKFGQF
                     ELIYQDISLNHEIKALNHEFQNNILRAIPLTKQPLIRVYLYKINNDLHELHIVIPHII
                     VDDASCEMVFCQFKNNYEKLTLGKELVSTPEKGSYFNYVKQNNNHYQKNLKNKIDFWQ
                     NYNKGFKMLSFGNRYHLSDTSKQTKHLFHYPIDPQHAEQFINWHKAQNINVSTGLIAA
                     CQIVFYKLSRQNKIPIILIHSGREGGEYKSTLGLFSEYKRINLTLNENGQFIDCIHSI
                     EEQLLKTAPYQKCSHLIKDSGLKGSSLSFGQYLTLALNKMFMLKHFKESKLHSIIIDY
                     YLKYLSRLVSFKKNISMKQRLNQLFKLDMSLQKPERLRVLVSVTPSFFTKERPDMSFA
                     NLDYSYPNHFGCMDRPIGNRTLWIYFSKNQDGEYQISINAPLTIECKNKIGSGLSQFI
                     TTFVENNNCNIAELIQAMEDSSIAS"
     gene            complement(11533..11934)
                     /locus_tag="VAH76_002145"
     CDS             complement(11533..11934)
                     /locus_tag="VAH76_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO1386751.1"
                     /translation="MKSLRMAGALFLFLFSLVSINVYAAPNTGASNAEMKDVCQLAGG
                     TFTSTIEGNWACCWDNWGCYGCVQGVCKMKCHTQRCRRANGQSAVLQPGEIKIKGKFG
                     TDIVVPKVSTQPSSSNPKQIKNNNTTVNQAQ"
     gene            complement(12164..>12425)
                     /locus_tag="VAH76_002146"
     CDS             complement(12164..>12425)
                     /locus_tag="VAH76_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386752.1"
                     /translation="NDPFIVFTSNIFAILGLRALYFLLVNMHKRFYFLKYGLAFILVF
                     VGFKMLIAHWFKIPIFIALGIVMATLVFCILFSIWHRKNEVT"
BASE COUNT         4020 a         2554 c         2393 g         3458 t
ORIGIN      
        1 agggagcgga tatggtattc ccatacaaaa tcttgctgaa ttctggctat acttaaggta
       61 aatagacctt ccaggttaat ttgtttatac tcaaattctc tctctgttat ggttgttttt
      121 attataactc tttgccagac aaataatttt tgtgcgtagc caggcacttt caggagcatt
      181 gtaagtggta aacgtaacta tgaaaaacaa aaccaaagac cttttgaatg aacccattgc
      241 catcattggg atgaattgtc aatttcctgg gattgattct gatatagaag atgttagtgc
      301 cttttatgac atgctgatga aagggcaaac tccaatcaaa gatgtgcctg aaaatcgctg
      361 gaatattgaa gaatactata gtgctgacag aaaaaaagca gataaaatta ttagcaaaaa
      421 aggcggtttt ttagataaca cccatttatt cgatgctgct tattttaaaa tatcttctgc
      481 cgaagcgaag caaatagatc cccaacaacg cctttttctc gaagtggcta ttcgagcttt
      541 aaatgatgcc aatatcccgc tagactcttt aaaagattct aacagcggag tttattgtgg
      601 catttccaca cacgaataca gccaactgaa ctataaagac catatcaaat ttaatgccta
      661 cacacccatt ggaatagcga acagtgctgc cgctggcagg ctctgccatt ttttgaattt
      721 aaaaggaccc agcatggctg ttgatacagc atgctcttct tccttcactg cactctatct
      781 agcggccacc gcattgagaa accagcaatg tgatatggca attgtcgggg gcgtccattt
      841 gagtctctgt cctgaaagtt ttatcggatt aaccaaagcc aatatgctgt cagcgacagg
      901 tcaatgcagc agctttgaca gtaaagcaga cggctttgtt cgtagcgaag gttgcggagt
      961 ggttattgtt aaacgcttaa gtgacgccat acgagacaat aacaaaatct atgcactcat
     1021 caaaagcatg gttattaatc aaaatggcga tggaacaggc cttgcagccc ccagtactaa
     1081 tgcccaaatt gccatgcacc aagccgcatt ggaacatgcc catttaactg caggtgaaat
     1141 tgattacatt gaaacccacg gaacaggcac aacagtaggt gatcccgtcg aatttaatgc
     1201 gatccaaagc attcaccagg ggcatcactc tgctcaaaaa cccttgatag taggagcact
     1261 gaaaagtaat ataggccata ctatttcatc atcaggcatt gcttctttaa ttaaagtact
     1321 ttgctctttg caaaatgaag cgattccccc caatttgcat tattcaaacc cgaacaactt
     1381 gattgatcct gaaagtatcc ctgcgcttct acccgttgaa gcaattcctt ttcccaaact
     1441 caaaaataaa aaaagatata tccaggtatc taatttcagc tttacgggca cgaatgtaag
     1501 tgtcattttg gaagaagcgc ctgagttgga atcacacgag tcaaagcaag acaacaatga
     1561 accaaaatgt tttgttgcct cagccaatag tgaattctca ttgaaacaat tgatgtcacg
     1621 ctacgaacac tatttgaaaa attcttcctc aaccttgggc gatatatgtc ataccttgat
     1681 caattgtaga gaccattaca aatttcgctg cgccatcatt gtaaccgata aaaaagaact
     1741 cattaaaaaa attgaatcga aagactatga aataaaaaag gtagttctgc aaaacaaccc
     1801 tagaacggta ttgaatgatg ccaaagccat ttacgacgct tacttatccg gtattaacat
     1861 cattactgat gatgaaaata ctgcatacaa caaagtagat ttaccgctct actgttttga
     1921 cagaaaaccc tattggcatg aagtcagaaa acaagtcaag aatgatcatt ggctggattc
     1981 tttatatcaa caaccaatgg cacaacaagt tgacgccatc aaagcagaga ttgcctctca
     2041 aatcaaagat cttttaaaga aagacagtat agatccacat caggatttgg aaactttggg
     2101 tttaactacc tccttattgg aatccctgga tcaaatcctt caggatatgt tttccccccg
     2161 atataaggtg ccttctctcc aatccttgcg ctacttaacc ctggataaac tggcaaggca
     2221 tttacaaaaa atgatcatgc cgcctgtcgt ttaccgacaa ccatctatta atgtactcaa
     2281 tgttgagccc atcgccatta tcggtatgag ttgtcgtttc cccaaagcag ccaatgtgga
     2341 tgaattcctg tctttgctgg aaaaaggcga aagtggcatg atcgatatcc ccttagagcg
     2401 ttgggataac gaaaaatttt atgactcgga tgtcgatgca ttaggaaaac tgtacatcaa
     2461 gcaactgggc ttcattgatc atgtcaacca ttttgatgcg gaatttttta atataagccc
     2521 gcgtgaggct aagctcatgt cacctcaatt gcgtgttttt atggaaacca gttatcacgc
     2581 acttgaagat gccaatctct ctcttgatgc ggtcaaagac actaatacag gagtttttgt
     2641 cggagtgggg actaatgaat atccccgagt tttagcttat caaggtgtta ccttggaaga
     2701 tttgaatatt tactttgcga cggggaatgt tttaaatgcg ctggccggtc gtgtagccta
     2761 ttcctttgac tttcatggtc caatacaagc aatagatacc gcatgctcat cctccatgac
     2821 agccatacac aatgcctgcc tgagtctgca atccggtgac tgtaatatgg ctcttgctgg
     2881 cggtgtgaac atcatcctta gccctgattc taacattact ctttcaaaag ccagaatgct
     2941 ctcacctgac agccgctgca aaaccttcag tgaagacgct gatggctatg gtcgcagcga
     3001 agggtgtggt gtggtggttt taaaacgttt aagtgacgcc gttaaagaca acgataatat
     3061 tctggcagta atcaaaggca cttccatcaa cagcgatggt aaaagtggtg gttttactgt
     3121 acctaatggt gctgcgcagg aagaagtcat cctaagtgca ttggcaaaag ctaacctgac
     3181 acctggtgat attgattttg tcgaagccca tgggactggc acaccattag ctgatcctat
     3241 cgaagtgaat acgctgacca agatttttag tgaacatcac aacaaagaaa acccgcttta
     3301 tatcagttca gtaaaaacca atattgggca ctcggaaagc gcatccggtg tagccagtgt
     3361 tatcaaaacc gttttaagcc tgaaaaccaa aacaatattt aagcacctga acttcaaaaa
     3421 gcttaatcct gccattcaat taattaatac agtaatccct cttgataaaa ccgattgggc
     3481 caagaaacaa gggttaaggt gtgccggggt tagctctttt ggttttagtg gggctaatgc
     3541 ccatgccatc atacaggaag ctcctgaaga caaaaaagaa gcacgcgccc ttcccgaaga
     3601 atctctgctc gttttatcag caaaaagtaa aacctctctg gagttattac tggccagcta
     3661 ccaaaactat ttatccaata cccgggaaga atttgccgat atctgctaca cagcagcaac
     3721 ttgccgcact cattttatgt atcgtgtagc aattagagcg caaaatgcaa cccaggcggc
     3781 caatatcata aaaaacaatg gatacactat ttgccatatc accaaagaaa aagaatgggc
     3841 tcaacaaccg gacacacttg cagaattgca aacagcctac ctggatggat tcctcatcaa
     3901 ctggtctgaa ttttatcgct cctttggcca tcattttgca aaagtaaaat tacccttata
     3961 cgaatttgag cgtatagaac attggtttgg tgacaaaaac aagctgaaag atgcgcctct
     4021 acccaaggac tggtgttttc aaatccagtg gcaaaatgaa ccatgtgata aaagcaatcg
     4081 aaagctgcga ggaaatagat ggcttttaat tggtcccaga catctggctg atggtttcaa
     4141 agctcaaggc ctggaaatta tctacgaaga tgaagagtat tctcgagaca aactggaagg
     4201 catcattttt gctgaaagtt tgcatctggc accacctgac atagaagcta atattgattt
     4261 tcaaaagaaa accttaaaaa agctgctgaa cttaattaaa gaacttgata accagtcaat
     4321 tgatttgcaa ttgctcattc ttaccagcaa tggaattgct gaactggcga atgacaaaca
     4381 ccccgtcaat ataagcaata gccctctcat tggtttctgc aagactctcg ctttagagct
     4441 gccacaattc aaaaccattc tcatcgatat ggataaaatc gaagaggaca aatacccgac
     4501 acaagtgata gaggaaatca attataacca tggaagccgc tatgaacaca tagtagctta
     4561 ccgtggtggg gaaaggctgg cttcccgttt aaaaaagact ccgctggaag ataggcggcg
     4621 attattgcat ggagtgaaag gacgctatct tattactggc ggctgcggtg gtttgggatt
     4681 ggtaactgcc caatccttgc tttcctctgg agcaagagaa ttaattctca cctcaagaaa
     4741 tatcgacaaa ccaaggatca aggaagccat ttataaaatt aaatcccatt accctggcgc
     4801 caacatccgc gttgtcagtt tggatattac tgatagggaa agtttaaaaa atttattatc
     4861 agagcttaat accgatggtc agttaaaagg catcatccat gctgctggtg ctgcgattaa
     4921 agcgcctttg cttgaacatc aggatgaaga tgttgattat ttattttcag ctaaagtaaa
     4981 aggcggatgg tatttacatg aactaagtct ggattgcaac ctggacttct ttgttgtcta
     5041 ttcttcagtt tcctccgtat ttggaagcaa caaagaatcc gtttatagtg gaacgaacag
     5101 tttcctcgac gccttgatag cggagcgcca gcgtatgggg ttacccggta ctgctgttca
     5161 atgggggcct tggggtgaag taggaatggc ccaaaaacgc tcgcgtgatg aaggactaaa
     5221 acaagcgtta atcagtaatg cacaaggtca tgtaatcatt aaatgcctca tcaacgacga
     5281 attaaaacac gccactatca tatctccaga atacctcaaa tttatgctgg attttgtacc
     5341 caagccccta cctgggtttt atggacaact tgatgaggaa cttgaaaaaa tacaacaaca
     5401 acatcatcaa agcccggcaa aaaatctgtc tccatggctt aatcaatatc tggaaattag
     5461 tgaagaggag cgatttaaag cctgtaaagc aatggtatct gatatttgca caggaatcct
     5521 tgaactgaca gaaaatgaag acttaaacga agacgaaggt tttttcgagt tgggttttga
     5581 ctcgctcatg attactgaat tggccagtga actaaagaaa aagctggaac cattccttaa
     5641 agttaccgtg accattggtt ttaattatcc atccatcagt aagctggcca aatacattga
     5701 atcagaactt gataagaact tggtcaagat acaaagtcca aaatcagctc ccaaaccgat
     5761 tgatgacggt atagcgatta taggcatgag ttgtttatta cctaatgcgc cagacatcgc
     5821 cgcctttgaa aaactgttgg aagaaggatt aagcggtatc aaagacattc caaatgaccg
     5881 ctgggataat aaccaatatt acgatcccga tatggaagcg cctggtaagt cctacgtcac
     5941 caaactggga ttaatcgaaa atattaaata ttttgacgct gcattttttg gtattagtcc
     6001 ccgagaagca aaattaatgg aacctcagca aagagtattt cttgagtgtt gctacaaggc
     6061 attggaaaat gccaactacc cttcggaatc tttgcgtggc agcttaacag gcgtatttgc
     6121 gggcgtaggt cccaatgaat attatgcgca actggaaaaa tcagggtttt caaatgaaga
     6181 actcagcgct tactctatca cgggtaatgt actcaatctg attccaggcc gagtagccta
     6241 cacttttgat tttaaaggcc cgtcaatcag tgttgacacg gcatgctctt cctcgttggt
     6301 ggcaatccat tacgcctgcc aaagtttaaa aaacaaggaa attgattatg ctcttgcggg
     6361 tggggtgaat atactgctta tgccggaatc taatgtaact ttgtgcaagg caaaggcatt
     6421 atcacctgat ggccaatgca aaacctttga tgaaagggct gatggatacg tcagaggcga
     6481 gggttgtggc gtagtattgc ttaaaaggct ctctgatgcc ttgcgggata acgatcatat
     6541 cctcgccgtt attaaaggct cagccgtaaa taacgatggt aaaacggctg gattaaccgt
     6601 gcctaatggt aaaagccagg aagaagtcat gaaaaaagcg cttggccaga ctgaattgtc
     6661 gagcaccgac atcagttata ttgaggctca tggcacagga actccgttag gggatcctat
     6721 tgaagtggat gcaatcaaca aagtctacgg tcatcaacgc aacaaagaga accccttata
     6781 cctgggtact gttaaaacca atattggaca tcttgaaagc gcatcaggcg tagcgggcgt
     6841 tataaaaacc gttatcagcc tgcaaaggaa aaccatttat aagaatttga attttaataa
     6901 attaaatccc aacatcaaac tggatggtac tcaactggcc ttagaaaaaa caaattggaa
     6961 tacgaataca aaactcaaat acgcagcggt caatgcgttt ggttttagcg gcacgaatgc
     7021 acatgttatc ctagcagagt ttcctaaaga agaaaaaccc aaaacctcaa aaccctctca
     7081 aaaacattta ctggttattt cagcccactc caaaacggct ttagataatt taactcagcg
     7141 ataccaacag tatttagaga cgactgaaca tcattttggt gacatatgct ttaccgcagc
     7201 gacttgtcga gaacattatc attaccgttt ggctttaacc gctgagaatg cagttgaagc
     7261 gagtcgtatg ctaggtgcag ggcaatttgc cttgtcttat catgaaaata ttacactgga
     7321 cttacaaaac gacagagcac tcaattccct gttaatggat tatctgcaag gaaaaccggt
     7381 tgattggaaa tcctattatc aatcccttaa aggagatttt acaaaagtcc tgctgcctaa
     7441 ttataccttt gaccgcagcg agtattggct ggataaaaaa aacaacgata gcatggccac
     7501 tcataaagat gagcaacacc ctcttttagg gcaaatgctt gccatgccag gaaatgaata
     7561 tctgtttcat cagaaactgg atttggagaa tttggcttat atcaaacaac acagtgtttt
     7621 cgaaaaagtg atttttccag caacagccta tattgaatca ggtttggcag cagcaaaatc
     7681 catatttcaa tccagcgttt ttcaaattga aaaattcaat atcgaacgtc ctctacatcc
     7741 taaacaaggt caggagtttc aattacaagt caaacctaaa atggacggac aatacaaaat
     7801 caatctattc gcgaagcaag agaacgactg gcaaactttc tgtgaaatgg aaatccatgc
     7861 caaaccgaca gcagcccggg aatctatcca tattgacgtg cttaaatcat cctttggcga
     7921 tagagtagag ctgtcggaca tatatgagca atttcagaaa agctcattat tttacggcga
     7981 tgaatttcat gtcctgcaag agggctatgt tcaggcgaac agtgttctgg ctaaagtcac
     8041 attgactaag gcaagccagg gccaggacta ttattatcat cctgttctgc ttgatggtgc
     8101 catgcaaagc atcttgttat taagtgttaa aaatgacgag aataaaaatt ttaacgccga
     8161 gaatattacc tacataccct atgccttcac ccgcatgact gtatttcaag aagcgccacg
     8221 cagcctatgg gtgcatttaa ctaaacaaaa tactgaagat gccagcgatc tttgcgttaa
     8281 tatgaattta tatgagcaat ctggtttgtt aattgctcaa attgcaggcc ttaaattgcg
     8341 aagggtaaca cgaagtcatt ttgtttccta tgacgctgct ttaagccacc tttattacac
     8401 ggaatggagc cctgtccctt ttaaaacctt acctcaccaa caagaaatcc ctgatctctt
     8461 ggtcattaca aaaaatcctg gcaaagctaa agaaatatta gatggcctta attatcatct
     8521 ggttgaaagg atcgatgact gcacaaatat agagaacagc aacatagtat tcctttatga
     8581 gcaaggacag tttcatgatc tttttcattg ctgccagatc ttattccgat tgcgtcccaa
     8641 gcgctttata ctggttacag aacatgctta tgccattcaa gaccttgatc aggttaatcc
     8701 ttaccacaca atggcctgtg ctttttggaa aagctttaga aatgaatatg agcccaataa
     8761 aaattatgcc attgatttgg gctccaggag tcgcttggct aaggcgttaa tgtatttatt
     8821 gaatgcagac acccatgaca ctcaaatagc agtcagagaa tctctttact tacccaggct
     8881 aaagaaaaag caattggtca gtaaccctga cccagagaga ttaattgatg gcaatgccac
     8941 ttatcttatt acaggcggta ctggaggatt agccaaaccc ttgatggaat atctgatgag
     9001 gcgcggtgcc aggcacattg ccattaccag ccgcacccac tatcccgatg acattaatgc
     9061 attaatagat aaggccaagc aaaataagat tgatatcaaa cattacatgg ttgatgccgg
     9121 aaattatcag aaaatggagc aagtgattgg agaaatacaa cagagcccca atccattgaa
     9181 aggcgtattt catttggcag gtttgataca agatgggctc atcgtcaatt tgaatgatga
     9241 agcaatgcaa aaagtaatca gcgcgaagat ggatggcgct ttaattttac atcaattaac
     9301 ccaaaatatc ccattagaat ggttagtcat gttttcttca tccgcttcct tgttaggcgc
     9361 cagaggtcag gcaaactatg cagcggctaa tgggttttta gatggtttag cccatttaag
     9421 gcgtcaacag ggcttaccag caatttctat caactggggc cctttccata ccaccggaat
     9481 ggcagctaat ttgactcata ctctacagca ttatggtttt ttactattgg ataaggacaa
     9541 cattgatatt cttgatgtgc tcttgcctag tcaattgcct caaatttctc cttgccctat
     9601 gaattgggat gtttactgca aacatacacc gaaacatatg gaattatctg agctggttaa
     9661 ggcgaaacca ctacccgatc agtctttttt gaatatactt agacaacatt ccaaagaaga
     9721 aagcatctca atattgtcac aagcattacg agaaattgca gcggatgtac tggctttaga
     9781 tgacagcgaa cacatttcga acgaccacga tttgttttca atgggaatgg attcactgat
     9841 gtcaatggat atcagaaatc gcatttatga caaattacaa tgccaaactc taagcttgcc
     9901 gattgagtat tttattaata ctcctaccat taataaaatc gccagacata tttctgatga
     9961 attagaccat gtttttgata gtcactctga ctatcgccct accgaaaatc cgccagaaag
    10021 agaaattgca ttatgtgatt ttcaatatgt attctgggtg cttaacaaat tggattacca
    10081 ttttaatatc ggtacacaaa tacaattaca cggcagactc aataaagatt atgtccaacg
    10141 ggcttttgaa tttgttgtga atcaaaacag tgtcttctgg atccatttta ataaggataa
    10201 accaacccaa aaattgcata aatttggaca atttgaatta atttaccagg atatttctct
    10261 gaatcatgaa ataaaggcgc tgaatcacga attccaaaac aatatattgc gcgctattcc
    10321 actcaccaaa caacccttga ttagagttta tttgtataag attaataatg acttgcatga
    10381 attacacatc gtaatacccc atattattgt cgatgacgca tcatgtgaaa tggttttttg
    10441 ccaatttaaa aacaattatg aaaaactaac tcttgggaaa gaattagtat ccactccaga
    10501 aaaaggctct tattttaatt atgtgaaaca aaataataat cactatcaaa aaaacctgaa
    10561 aaataaaatt gatttttggc aaaactataa taaaggcttt aaaatgctga gtttcgggaa
    10621 tagatatcat ttatccgata cctcaaaaca aacaaagcac ttatttcatt accctattga
    10681 tcctcaacat gccgaacaat ttataaattg gcataaagcc caaaatatca atgtcagtac
    10741 tggattgatt gccgcatgcc aaattgtttt ctacaagcta agccgccaaa ataaaatacc
    10801 gatcatttta attcacagtg gtagagaagg tggggaatat aaatcaacct tgggtttatt
    10861 ctcggaatat aaaaggataa atcttacctt aaatgagaat ggccaattta ttgattgtat
    10921 tcattcaatt gaagagcaac tcttaaaaac agctccctat caaaaatgct ctcatctcat
    10981 caaagacagt ggcttgaaag gatcgagcct atcctttggt caatatttga ctttggcgtt
    11041 aaataagatg tttatgctga aacactttaa agaaagtaag ctccattcca ttatcattga
    11101 ctattacctg aaatatttaa gtcgcctggt atccttcaaa aaaaacattt caatgaaaca
    11161 acgactaaat caactattta aattggatat gtcattgcaa aaaccggaaa gattaagagt
    11221 tctcgttagc gtaaccccca gcttttttac caaagaacgc cctgatatga gttttgctaa
    11281 tcttgattac agctatccta atcattttgg atgtatggat aggccgatag gaaatcgaac
    11341 gctttggatt tatttctcca aaaatcagga tggtgaatat caaatttcca ttaatgcgcc
    11401 attaacaata gaatgtaaaa ataaaatcgg aagcggtctt agccagttta ttacaacttt
    11461 tgtagagaat aataattgta atattgctga attaattcaa gcaatggagg attcctccat
    11521 tgcttcctga ttttactgag cctggttaac cgtagtatta ttatttttta tttgtttagg
    11581 gtttgatgat gaaggctgag ttgaaacttt gggtaccaca atatccgttc caaatttgcc
    11641 ttttatcttg atctcacctg gctgcaatac agcagattgg ccattggctc tacgacaacg
    11701 ctgggtatga catttcatct tacaaactcc ctgaacacat ccatagcatc cccagttatc
    11761 ccaacagcaa gcccaattgc cttcgattgt tgaggtaaaa gttccccctg ccaactgaca
    11821 tacatctttc atttcagcat tcgatgcccc cgtatttggc gcagcataaa cattaattga
    11881 tacaaggcta aagagaaaga ggaaaagcgc ccctgccatc cttaaagatt tcatgattca
    11941 cctccttgga atcgtactat caggatttgc taaaaaaccc aatttcattg ttaaaaagta
    12001 ctttcaattt ggtcatttag tttatctaaa ctccctcatt aaaaacattt tttgccttga
    12061 ccttggggct tttcgtaagc cctgactatt taattatggc aaaacctcac aattgttcaa
    12121 gttttaaaca aaaagaggtc ttgaaacact gaaaagcccc ccctcaggtt acttcatttt
    12181 ttctatgcca aatactgaat agaatacaaa aaaccaaagt tgccataaca atacccagag
    12241 caatgaaaat cggtatcttg aaccaatgtg cgataagcat cttaaagccc acaaatacta
    12301 aaataaaggc aagcccatat ttgagaaaat aaaatcgttt gtgcatattc acaagcagaa
    12361 aatataaagc gcgtaatcct aaaattgcaa atatattaga agtaaatacg ataaaaggat
    12421 cattg
//
LOCUS       DATNSN010000101        12368 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.101, whole genome shotgun sequence.
ACCESSION   DATNSN010000101 DATNSN010000000
VERSION     DATNSN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.101"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            80..694
                     /locus_tag="VAH76_002147"
     CDS             80..694
                     /locus_tag="VAH76_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386753.1"
                     /translation="MTSSKGKNILHYTLRKKRKHERMSHYPKGDRIDRSTGSQYFYHC
                     HRENFESNEHGHFHCFLRYKHIPKRIKPAPLEDWDKYIDNPMTHLVAIGMNQFGQPIR
                     LFTVNRWVTSEIWYGAEHIPYFLKSYKMTLIDDPYWQVLDQWVEGMLHLFAPQIAWLH
                     QERDKRIQLHQLNSPNDNPYTNHELEELSEINIDLKKQIEWVIS"
     gene            847..3189
                     /gene="purL"
                     /locus_tag="VAH76_002148"
     CDS             847..3189
                     /gene="purL"
                     /locus_tag="VAH76_002148"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEO1386754.1"
                     /translation="MQNLTETLDFSSLNRGEIEKLLRDYNLNLSVDEALTIQNEFLKR
                     PPTISECVLWSIQGSEHCSYKSSRIHLKQFNTSGPHVILGAKEDAGIVSVAQDKNGYR
                     YGVIVSHESHNHPSQIVPYEGAATGVGGNVRDVCCMGGEVIAVADSLRFGDIKRARTH
                     WIQEGVVSGIAGYGNPLGIPNIAGDVYYDPAYNENCLVTVVTLGIVREDHIIHSYAPP
                     EAENYVFILVGKPTDNSGFGGASFASTVLEEDSQEKNKGAVQEPNAFLQRHLLKANYS
                     LFQLLREKNLIDKVGFKDLGAGGVACASIELAEAGGSYGAEIDLDKVPTGMPGLMPSV
                     ILCSETQERFMWVVPPDLVDTILKHYNETFALPQVSEGACAAVIGKIRTDGLYVVNYK
                     GRELVRAKVPDVTKGIVYNRPHASSQKQNTEPVFLPPDDYNQILLQLLAHENIASKEP
                     IFEMYDKQVQGRTLIQAGWADAGVLQPFNESKYPEEIRKTGIALSLDQNPRYNKIDAY
                     WGAVNAVVESVRNITAVGATPVAITDCLCFGNPEKPEQMREFVDSVRGISDACAAVHL
                     KDHPQSTLPVIAGNVSLYNESVKGAIPPSPMISCLGTLPDIDFAITFDFKKSDSLLIL
                     IGERKDECGGSVYYQLHNELGSNVPKPDLSLFNREIHAVSSAIQHGLVNAAHDVSEGG
                     VAVALAEMSFKNSLGVAVQINGELSADKLLFGETGGFILEIDKQHKAAFDKLVTQYQV
                     PYMVIGHTTEQPVLQMNSVINLPVEEARQAWENGLRERLL"
     gene            3189..4232
                     /locus_tag="VAH76_002149"
     CDS             3189..4232
                     /locus_tag="VAH76_002149"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO1386755.1"
                     /translation="MSTINYKSAGVDIEAGNEAVARIKRNVEKTFSPQVLTGIGSFGA
                     MYDLKPFMEKFKHPVMVQSIDGVGTKTIVARMMQKFDTIGIDLVSATTNDIIVLGARP
                     LTLLDYIATDRLKPEYIEEIIIGMTQACLENNISLVGGETAEMPDTYLKGEHDLVGII
                     TGVVEKDEAILGKDITPGDSVLAFPSSGLHTNGYSLARKLLFELGGYTVESQLDELDA
                     TVGEALLVPHINYTNPVLHLLNNKIPIKGMAHITGGGVLENIPRVLPNHCSVEINKNS
                     CPVLPIFNVLKALGKLDDQEMYRTFNMGIGLVMIASPGVVSEIYTALKEFPNFPVYEV
                     GKVVSGKPEVRLL"
     gene            4232..5491
                     /gene="purQ"
                     /locus_tag="VAH76_002150"
     CDS             4232..5491
                     /gene="purQ"
                     /locus_tag="VAH76_002150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HEO1386756.1"
                     /translation="MKIAVVQFPGSNCERETMLAVQRAGMTPVEFLWNDSSQKLREMD
                     GYIIIGGFSYEDRSRAGIIAALDPVMKEIKLQSELGKPVLGICNGAQILVETGLVPGL
                     KNYQIGMALTENKRIQDGKILGTGFYNSWIHMRTGDRVRQNAFTRYLSKDSVLSIPAA
                     HAEGRFVMPVKLLQEIEEEGLNAFQYCDAQGNRDDNFPVNPNGSLRNIAAIINKAGNV
                     MAIMPHPERTFAGDPIFHSMKEYIKEKKTFKQQTLSYQPEPVKIIPYDKKDNAHELIV
                     KLIITDNAALTVQNTLRQLGIPVTVNRMMYWHVDTNFPETIEKIKQTGVLYNDRKEYL
                     VTSEHIFSSAAKAFLVKSKDDMIGQQKLQMLRDHFSIDGVDAIHHGVLWMFSSENVKI
                     QELAESILATNIIFNPNAHICYEYQCP"
     gene            5472..6452
                     /locus_tag="VAH76_002151"
     CDS             5472..6452
                     /locus_tag="VAH76_002151"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO1386757.1"
                     /translation="MNTNVPSQEDLLAALPFCLTETSLPFGKKYKGKVRDTYDLGDQL
                     ILVTTDRQSAFDRCLAAVPYKGQVLNLTSAWWFKNTQSIVPNHLIAVPDPNVAIAKKC
                     KIFPIEFVVRGYISGSTSTSLWTQYQKGVREYCGITFPDGLRKNQKLESPVITPTTKE
                     TLHDRPISPHEIVAEGWMTQEDWDETSSYALKLFQHGMEVAQQHGLILVDTKYEFGRD
                     AEGRIVLVDEIHTPDSSRYWLFNGYQERFDAGKEPENIDKEFLRLWFVDHCDPYKDEV
                     LPQAPQELIVTLASRYIQLYEMITGESFVYDSNPGPVNDRILHNIQRWLG"
     gene            6475..7974
                     /gene="purF"
                     /locus_tag="VAH76_002152"
     CDS             6475..7974
                     /gene="purF"
                     /locus_tag="VAH76_002152"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO1386758.1"
                     /translation="MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFK
                     HGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGN
                     LVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
                     EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYA
                     LGFEPKGDLQPGEVAYVNQQGQLYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRA
                     RLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGIRYSEGLYKNPFIGR
                     TFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEI
                     YFVSTCPPIKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSR
                     RGQYDMSKPCMACMDGKYICGQITEEKIKLLEKKRLTENDQETEIGEMS"
     gene            7971..9290
                     /gene="purD"
                     /locus_tag="VAH76_002153"
     CDS             7971..9290
                     /gene="purD"
                     /locus_tag="VAH76_002153"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO1386759.1"
                     /translation="MINILIVGSGAREHAIATALSKSENKPKIYCFGTTRNPGIQRLT
                     SDYWVGDITDPEQVNQQAKEWEINLAIIGPEAPLEHAVADALWDCGIPVIGPRKNLAK
                     IETSKAFARELMEKYGIPGLPKYKVFTSLEGIGEFLSELGENNYVVKANGLMAGKGVK
                     VGGEHLLSFEEALNYCKEIFTHNQSLVIEEKLIGQEFSFMCFSDGISLVPMPIVQDHK
                     RAYEHDEGPNTGGMGSYSAADHSLPFLSPEDVRAAFEINQSVIKALMTEMSDKYIGIL
                     YGGFMATAHGVYVIEFNARFGDPEALNVLSLLESDFVKLCVAMANGNLQKESVRFLNQ
                     ATVCKYAVPDGYPDNPLRHVEIDISAVEPKDTLYLAAVNFKNGKLYATGSRTAAYVGI
                     ANTISEAEEIAEQQIKNIKGPLFHRKDIGTSPLINTRVEVMRRLRAL"
     gene            9287..9865
                     /locus_tag="VAH76_002154"
     CDS             9287..9865
                     /locus_tag="VAH76_002154"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO1386760.1"
                     /translation="MIRLGILGSTRGTNMLALVDAINEGTLKAKIELVISNKPDAIIL
                     ERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNN
                     QVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
                     EGDTAQTLKARVQQLEGIALVDAINLIASKGL"
     gene            complement(9862..11979)
                     /locus_tag="VAH76_002155"
     CDS             complement(9862..11979)
                     /locus_tag="VAH76_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO1386761.1"
                     /translation="MGYHFLTEGNGIIACYDMYPTQFHSIKDMINYLPVLKQMGFNAL
                     WINPMQMPGDISGFFKTDKNNGVKTGNEVTRSLYAMSHPLLFNPQFSLGSPEDPMGTT
                     QRLNSEALQLFTQNARNLGIVPMFDLVLNHVAIDSPLCQDKPHWFKGVHEDFKDVRGF
                     NYDDESIRKEIITEFWQPYIKRYMIEYGFDGVRVDAVGYVHPAVRSEIYAYIHSLAAE
                     YGKPKPVILDEALFSKRPLADEVNYLKLPGIGPTHITTEAYNVEFDLASADLPYEITL
                     EEQLKASVVFQQKDGTWRENTKGGCINFCGNHDYRSLAMTILFQMAKKRLESDAFYND
                     LMSSYQDLYFKFNAEQEKPHELKEPLKTTLLYSYVDQIKKELEDNKDETTQEFKTLVL
                     EKLALSALTASGGWFLLSGDETCDITAKTVFQRKGAVDKSYYPQREHRIFSEKPVIAH
                     KILEKMAEENFFIENSENKWMQELYRSLSSFPEMQKRLLVSHIDNIKHQINAGIDHVQ
                     EKFSRLLASENLNIGFTSQDFLENPRTHENGWLGLQNNFLFIKQLNTLLKRLPASLPG
                     FSSELVRLPEKPHLIIIVRKNGNELNAPIDVVVVNLEQEKRQTLTKEDFQLIAKNYCN
                     QYFSKNRPSVCDSLREKLYSHVLKSAVNNRVHTDTSIKLNGIHLFEFKETSLNTFFTI
                     EKEKNYSHGNEDNTALTGNTISH"
     gene            complement(12083..>12368)
                     /locus_tag="VAH76_002156"
     CDS             complement(12083..>12368)
                     /locus_tag="VAH76_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386762.1"
                     /translation="IARKLSEHQYSISAINNAYFGKGLIHGNKTQIESWDKAISETIA
                     KGKIPSTLLNKIYDKASEKAKGILDEFTVEVKDMPEEEKLENEVAPTLSI"
BASE COUNT         3765 a         2148 c         2647 g         3808 t
ORIGIN      
        1 taacttaccc atattgtcca gctggtataa aaagaaattt cttggttatg cttcacaagt
       61 tcttgaagcc caacaaaaaa tgacatccag taaaggtaaa aatattctgc actacacgct
      121 tagaaaaaag cgaaagcatg aacgcatgag tcactatcct aagggagaca ggattgatcg
      181 tagtactggc tcacaatatt tttatcattg ccatagagaa aatttcgaaa gtaatgaaca
      241 tggtcatttt cattgttttt tgcgatataa acatataccg aagcgtatta aaccagctcc
      301 tttagaggat tgggataaat acatagataa tcccatgact caccttgttg cgataggtat
      361 gaatcagttt gggcaaccga tacgattatt tacagtcaat cgttgggtaa catcagaaat
      421 ttggtatggt gctgagcata ttccatattt cctgaaatct tacaagatga cattaataga
      481 cgatccttat tggcaagtat tagatcagtg ggtggaaggc atgctccatt tgtttgctcc
      541 ccaaattgcc tggcttcatc aggagcgaga taaacgcatt cagcttcacc aactgaatag
      601 cccaaatgat aacccgtaca ctaaccatga gctggaagag ctttctgaga taaatattga
      661 tttgaaaaag cagattgagt gggtaataag ttaaattcca aaattagaac cacaacttaa
      721 tttatcttaa ttattggttt ttcattgctt ataccccgtg taaaaatata caatatctac
      781 tgctttgggg agagattagt cctttactga tcaatcagag attaattcta tctggtatag
      841 agattcatgc aaaatcttac tgaaaccctt gatttttctt ctttgaatcg tggagaaatt
      901 gaaaaattac tgcgcgatta taatttaaat ctttctgtag atgaggcgtt gactatacag
      961 aatgaattcc tcaaaagacc cccgactatt tcagagtgtg tactttggtc tattcaagga
     1021 tcagaacatt gttcttataa aagcagccgt attcatttaa agcaattcaa tacctcaggc
     1081 cctcacgtga tactgggcgc caaggaggat gcaggtatag tatctgtagc ccaagataaa
     1141 aatggttata gatatggcgt tattgtcagt catgaatcac ataatcaccc atctcaaatt
     1201 gttccctatg aaggcgcagc taccggagtt ggtggtaatg tccgtgatgt ctgttgtatg
     1261 ggtggggaag ttatagcagt agcagacagt ttgcgtttcg gagatatcaa acgtgcacgt
     1321 actcactgga tacaggaagg ggtggtttca ggtattgccg ggtatggcaa tcctttggga
     1381 ataccgaata tagctggtga tgtgtactat gatccggcct acaatgaaaa ttgtttggtt
     1441 actgtagtca ctctggggat agtaagagag gatcatatta tacattctta tgctccgccc
     1501 gaagctgaga actatgtttt tattttagta ggtaagccaa ctgataatag tgggtttggc
     1561 ggcgcgagct ttgcctcaac agtattggaa gaagattcac aagaaaagaa taagggagct
     1621 gttcaagagc ccaatgcttt tttacagaga catctcctta aagctaatta ttcattattt
     1681 caattactaa gagaaaagaa tttaattgac aaggtaggct ttaaagattt aggagcaggt
     1741 ggcgtggcct gcgcaagtat cgagttggct gaagccggcg gttcttatgg cgccgaaatt
     1801 gatctggaca aggtacccac aggaatgcca ggattaatgc cttcagtcat tctatgctct
     1861 gaaactcagg agcgttttat gtgggttgtt ccccctgatt tagtagatac tatcctgaaa
     1921 cactataatg aaaccttcgc gttaccacag gtttctgaag gggcatgcgc tgcagttatc
     1981 ggcaaaattc gtaccgatgg attatacgtg gtgaattaca aagggcgaga acttgtgaga
     2041 gctaaagtcc ctgatgtcac gaaaggcatt gtttataaca ggccacatgc ctcttcgcag
     2101 aagcaaaaca cagaaccagt ttttctccca ccagatgatt ataatcaaat tttgttgcag
     2161 ttattggccc atgagaatat agcgagcaag gaacctattt ttgaaatgta tgacaagcaa
     2221 gtgcagggtc gcaccctcat acaagcagga tgggctgatg ctggagtatt acaaccattt
     2281 aatgaatcga agtatcctga ggaaattcgt aaaacaggta ttgccttgtc tttagatcaa
     2341 aatccccgct ataacaaaat tgatgcctat tggggagctg ttaacgcggt tgtcgaatcg
     2401 gtacggaaca tcactgcagt tggagctact ccagtcgcaa taacagattg cttgtgtttt
     2461 ggtaatccgg aaaagccaga acaaatgcgc gaatttgttg attctgtgcg aggaattagt
     2521 gatgcctgtg ccgctgttca tctgaaagat catccccaat caactttgcc tgtcattgcc
     2581 gggaatgtct cattgtataa tgaatcagtg aaaggggcta ttcctcccag ccccatgatt
     2641 agttgcctgg gaacgcttcc agatattgat tttgctatta cctttgattt taagaaaagt
     2701 gatagcctat tgatattgat tggagagcgt aaagacgaat gtggtggtag cgtttattat
     2761 caactccata atgaattggg ttctaatgtt ccaaaaccgg atttaagctt gtttaataga
     2821 gaaatccatg ctgtttcatc ggcaattcag catgggttgg ttaacgctgc tcatgatgtc
     2881 tcagagggag gggttgctgt tgctcttgct gagatgagtt ttaagaactc cctgggagtt
     2941 gctgtacaga ttaatggaga gttatctgcg gacaaacttt tatttggaga gacaggcggt
     3001 tttattcttg aaatcgataa acaacataaa gccgcttttg acaaactggt tactcaatat
     3061 caagtccctt acatggttat cggacatact acagagcagc cagttttaca gatgaattcg
     3121 gttatcaact tgcctgtcga agaagcgagg caggcttggg aaaatggctt aagagagagg
     3181 ctattataat gtctactatc aattacaaat cagcgggtgt cgatatcgag gcgggaaatg
     3241 aagctgtcgc acgcattaaa cgtaatgttg aaaaaacatt ttcgcctcaa gtcctgacag
     3301 gaatcggtag ttttggcgca atgtatgatt tgaagccttt tatggaaaaa ttcaaacacc
     3361 ccgtcatggt tcaaagcatt gatggcgtag gcacgaagac aatagttgct cggatgatgc
     3421 aaaaatttga tacaatcgga attgatttgg tcagtgcgac caccaacgat attattgtgt
     3481 tgggtgcaag gccattaaca cttttggact atatagcaac tgatcgattg aaacctgaat
     3541 atattgaaga aattattatt ggcatgacgc aagcttgcct cgaaaataat atttccctgg
     3601 tcggtggtga gacggctgaa atgcctgata cctatctgaa gggagagcat gatttggtag
     3661 gtattatcac tggtgtagtg gaaaaggatg aagccatact aggaaaagat ataactccag
     3721 gcgattcagt cttggcattt ccttccagtg gcttacatac caatggatat tcacttgcca
     3781 gaaaactttt gtttgaacta ggtggatata ctgtagaatc ccaacttgac gagttggatg
     3841 ctacagttgg tgaggcttta ctggtacctc atatcaatta tacaaatcct gttctacacc
     3901 tgttgaataa caaaatccct atcaagggaa tggctcatat tactggcgga ggtgtactgg
     3961 aaaacattcc gcgagtatta cctaatcatt gttccgttga aatcaataag aattcatgtc
     4021 cggtgttgcc gatctttaat gtgctaaaag cattggggaa actggatgat caggaaatgt
     4081 atcgtacttt taatatggga ataggattag taatgatagc ttcccctggt gttgtttctg
     4141 aaatttatac agcgctaaaa gaattcccta atttccctgt ttatgaagta ggaaaagtcg
     4201 tatcaggaaa accagaagtg agattattgt gatgaagatt gcagttgttc agtttccagg
     4261 ctccaattgc gaacgtgaga caatgcttgc ggtgcaacga gcaggtatga ctccagttga
     4321 gtttctgtgg aatgattcat cacaaaaact gcgagaaatg gatggttata ttattattgg
     4381 tggtttttca tatgaggata gatcaagagc aggaattatt gcagcccttg atccggtaat
     4441 gaaagaaata aaattgcaga gtgaattggg aaaacctgtt cttggtattt gcaatggagc
     4501 acagatcctt gttgaaacag gcctcgttcc tggtttaaaa aactatcaaa ttggtatggc
     4561 tctgactgag aataagcgaa ttcaggatgg aaaaatttta ggaacaggat tttataacag
     4621 ctggattcat atgcgaaccg gtgaccgagt gaggcaaaat gcattcaccc gctatttatc
     4681 aaaggattct gtattatcaa ttcctgctgc ccatgctgaa gggcgttttg tgatgcctgt
     4741 taaattattg caagaaattg aagaagaagg tttaaatgcg tttcaatatt gtgatgcgca
     4801 gggaaacaga gatgataatt tccccgttaa tcctaatggt tctctacgga atattgccgc
     4861 tattattaat aaagcgggta atgtcatggc cataatgcct caccctgaac gtacatttgc
     4921 gggagatcct atattccatt caatgaaaga atatattaag gagaaaaaaa cattcaagca
     4981 gcaaacatta tcttatcaac cggagccagt taaaataatt ccttatgaca aaaaagacaa
     5041 tgcccatgag ttgattgtta aattgattat taccgataat gctgctttaa cagtacaaaa
     5101 tacgctaaga cagttaggta taccagtcac agttaatcgt atgatgtatt ggcatgtcga
     5161 tacaaatttt cctgaaacaa tcgaaaaaat aaagcagaca ggggttttat ataatgatcg
     5221 gaaagagtat ttggtaacgt ctgagcatat attttcatca gcagccaagg cttttttggt
     5281 taaatcaaaa gacgatatga tcgggcaaca gaagctgcaa atgcttcggg atcatttctc
     5341 aattgatggc gttgatgcca tacaccatgg tgttttgtgg atgtttagca gtgaaaatgt
     5401 taaaatacaa gaattggcag aatccatttt agctactaat attatcttta accctaatgc
     5461 tcatatctgc tatgaatacc aatgtcccta gtcaagagga tcttcttgct gcgttgcctt
     5521 tttgcttaac cgaaacctct ttacctttcg gcaaaaagta taaagggaaa gtaagggata
     5581 cctatgatct cggcgatcaa ttgattttgg taactacaga tagacaaagt gcctttgatc
     5641 gatgccttgc tgctgtacct tataaaggac aagtattgaa tttgacttca gcatggtggt
     5701 ttaaaaacac gcaatcgatt gtgcctaacc atttaattgc cgtacctgat cccaatgtgg
     5761 cgatagccaa aaaatgcaaa atattcccta ttgaatttgt tgttcgtggg tatatttcgg
     5821 ggagtacaag tacgtcttta tggacgcaat accaaaaagg agttcgtgag tactgtggta
     5881 taacgtttcc tgatggatta agaaaaaatc aaaaacttga gagcccagtg ataaccccca
     5941 ctactaaaga gacgctccat gacaggccga ttagcccaca cgaaatcgtc gcagaaggct
     6001 ggatgaccca agaagattgg gatgaaacca gcagttatgc tttaaagtta tttcaacatg
     6061 gaatggaagt agcacagcaa catggtttga ttttggtgga taccaaatat gaatttggcc
     6121 gtgatgcgga aggaagaatt gtattagtgg atgaaattca tactccagat tccagtcgtt
     6181 attggttatt taacggttat caagagcgat ttgatgccgg taaggagcct gagaatatag
     6241 ataaagaatt tttgcgcttg tggtttgttg atcactgcga tccttataag gatgaagtat
     6301 tacctcaagc accacaggag ttaattgtta ctttagcatc aagatatatc caattatatg
     6361 aaatgattac aggtgaatcg tttgtgtatg acagtaatcc tgggccagta aatgatagga
     6421 ttttgcataa tatacaacgt tggttaggtt aatttttagc cgggggaaag gcttatgtgt
     6481 ggcattgtag gaatttacag tcatgaaccc gttgctgctg agctttatga tagcttgatt
     6541 catttacagc accgggggca agatgcctca ggtattttaa ccggagctga tcgtttttac
     6601 ttcaaacatg gacttggact ggtcagagag tctattttct ctgaagatat tcttaaactg
     6661 aaggggaata tcggcattgc tcatgtgcgt tatcccacgg ccggaggtta tagtgaagcg
     6721 gatgttcagc ctctttggat agggagccct cgcggtatag ctttggctca taatggaaat
     6781 ttagtgaatt atggagagtt ggttgatgat ttatgcggaa aacagcatag gcatttaaac
     6841 tctactcttg attctgaggc acttttatta cttctggcag actatttagc caagggatct
     6901 tattccgatc atgatgaaga gcaattcttt caattactat gtatggcagt taatgaaata
     6961 catcaacgag ttcagggttc ctattctgtt gtgagtttag taattggcaa aggtttggtt
     7021 gcatttaggg atccgcatgg tatccgtcct ttagtttggg gtgagcgagt ccatgaaaat
     7081 ggtagtaaag attatatttt tgcttcagaa accacccctt tttatgcttt aggttttgag
     7141 cccaagggtg atttgcagcc cggtgaagtt gcttacgtga atcagcaagg acaattgtac
     7201 agaaaagtgc tttcatcaaa aaaatttacc ccttgtatat ttgaatacgt ttacttcgcc
     7261 agaccagatg ccacattaga cgatgtgagc gtttatcgag ccagattacg tatggggcaa
     7321 aatttagcta aaagctggaa aaagagattt ccagatacac tacctgatgt ggtgattcca
     7381 gccccttcaa cagcaaatac tgccgcatta gcttttgcaa atgagttggg aatacgttat
     7441 tccgaaggtt tatataaaaa tccttttatt ggacgtactt ttattatgcc taatcaggag
     7501 tcgcgatcaa gacaggttcg ttataaatta actcctcaga aaacggaaat taataaaaaa
     7561 aaggtattaa tcgttgatga cagtatcgta aggggaacta cctcacgcga aatagtcaga
     7621 atgatacgag aatatggtgc atcagaaata tattttgtat caacctgtcc accaattaaa
     7681 aacccttgtt tctacgggat tgatatccca tcgcgtaaaa aattgatagc agcccataaa
     7741 acggaagagg aaatccgtca atttctaggg gtggataaat tattatacca aactcaggaa
     7801 aaccttatag aggctgtgtc tcgcaggggg caatatgaca tgagcaagcc ttgtatggct
     7861 tgtatggatg gtaaatacat ttgtggacag attactgaag aaaaaattaa attgttggag
     7921 aaaaaaagat taaccgagaa tgaccaagaa acagagatag gagagatgtc ttgatcaata
     7981 tccttattgt tggttcaggt gcgcgtgagc atgcaatagc tacagcattg agtaaatcag
     8041 agaataaacc aaagatttat tgctttggaa caactcgtaa tcctggtatc caaagactca
     8101 cctcagatta ttgggtaggt gatattacag accctgagca agttaaccag caagcaaaag
     8161 aatgggaaat taatttggcc attattggtc cagaagcgcc tttggaacat gctgttgctg
     8221 atgctttgtg ggattgtgga attcctgtta taggccccag gaaaaatttg gctaaaattg
     8281 aaaccagtaa agcgttcgcc agagagttga tggaaaaata cggtattcca ggtttgccta
     8341 agtataaagt atttaccagt ttagagggaa taggggagtt tttatcagag ttaggtgaaa
     8401 ataattacgt tgttaaggcc aatggtttaa tggcgggcaa gggtgtaaaa gtaggtggag
     8461 aacatctgtt gtcatttgaa gaggctttaa actattgtaa agaaattttt actcataatc
     8521 agtctttagt cattgaagaa aaattaatag gtcaagaatt ttcatttatg tgtttttctg
     8581 atggcatctc attagttcca atgcctattg tgcaagatca taaacgagct tatgagcatg
     8641 atgagggtcc taatacaggt ggtatgggga gttattcagc ggcagatcac agcttgccat
     8701 ttttgtctcc agaggatgtt cgtgctgctt ttgaaattaa tcaatcagta atcaaagcgc
     8761 ttatgacgga aatgtctgat aaatacatag gaatacttta tggcggattt atggccaccg
     8821 cacatggggt gtatgtgatt gagtttaatg ctcgctttgg agatcctgag gctcttaacg
     8881 tgcttagctt gttggaatct gattttgtta aattatgtgt tgcgatggct aacggcaatt
     8941 tacaaaaaga atccgttcgc tttttgaatc aagccacagt ttgtaaatac gctgtccctg
     9001 atggttatcc tgataatcca ttgcgccatg ttgaaattga tatttctgct gtcgaaccta
     9061 aagatactct atatttggct gcagtgaatt ttaagaatgg caaactctat gccacaggtt
     9121 cgaggacagc cgcttatgtc ggtatagcga atactatcag tgaggcagaa gaaattgctg
     9181 aacaacagat aaaaaatata aaaggccctt tatttcacag aaaagatatt ggcacatcgc
     9241 ctttaattaa tacacgcgtt gaagtaatgc gtaggttgcg tgccttatga ttcgtttagg
     9301 aatattagga tcgactcgtg gaaccaatat gttggctctt gttgatgcaa tcaatgaggg
     9361 aactcttaag gcaaagatag agcttgttat tagtaataaa cccgatgcca ttattctgga
     9421 aagagccaaa tcgttgggtt tgaatgcaca gtttgtcaat cctgagggat taaacagaat
     9481 tgattttgat aaaaaagtaa gtgacatact gatcaaccat cagattgatt tgattgtttt
     9541 gattgggtac atgagaattt tgtcggcgga tttcgtaaat aaatggaata atcaggtaat
     9601 taatgtacat ccctctttat tgccagcatt tgcagggaaa atggatatgg atgtacatca
     9661 agctgttctt gattccggcc taaaggaaac aggttgtact atccattttg tcaccgaaga
     9721 ggtagatgcg ggtcctgtta ttttgcaaaa aaaatgccct gttttagaag gagatactgc
     9781 tcaaacctta aaagcaaggg tgcagcagtt ggaaggaata gctttggtag atgcaattaa
     9841 cctgattgca tcgaaaggtc tctaatgcga gattgtattt ccagtcaaag cagtattgtc
     9901 ttcatttcca tgagaatagt ttttttcttt ttcaattgta aaaaaagtgt ttaacgaagt
     9961 ttctttaaac tcaaataaat ggattccatt cagtttaata ctggtgtctg tatggactcg
    10021 attatttaca gcagatttta aaacatggga atataatttt tcgcgtaaac tatcgcaaac
    10081 actcggtcta tttttagaaa aatattgatt acaatagttt ttggcaatta attgaaaatc
    10141 ttctttagtt aatgtttgtc gtttttcctg ttcaaggtta acaacaacaa catcaattgg
    10201 agcatttaat tcattaccat tttttcttac gataataatg agatgcggtt tttcagggag
    10261 tcgaaccaac tcactggaga atccgggtag tgatgcaggt agtcttttta ataatgtatt
    10321 taattgtttg ataaatagaa aattattctg taatccaagc catccatttt cgtgggttct
    10381 tggattctct aaaaaatctt gagatgtgaa acctatattt aaattttcac tggcaagtag
    10441 cctggaaaat ttttcttgta catgatctat gccggcattt atttggtgtt taatattatc
    10501 aatatgagat actaaaagac gtttttgcat ttcaggaaaa cttgataaac ttctgtaaag
    10561 ttcctgcatc cacttatttt ccgagttttc tatgaagaaa ttctcttctg ccattttttc
    10621 taaaattttg tgagcaataa ctggtttttc actaaaaata cgatgttcac gttgtgggta
    10681 ataagattta tcaacagcac cttttctctg aaatactgtt tttgcagtga tatcgcatgt
    10741 ttcatcacca gataataaaa accatccgcc actggctgta agagcggata aagccagttt
    10801 ttctaatacc agagttttaa attcttgtgt tgtttcatct ttattatctt ccaactcctt
    10861 tttgatttga tcaacataag agtaaagtaa ggttgttttc aaaggctcct tgagttcatg
    10921 tggtttttct tgctcggcgt taaacttaaa gtacaagtct tgataggaag acattaagtc
    10981 attataaaaa gcgtcagatt ccagtctttt tttggccatt tgaaataaaa tagtcatcgc
    11041 caatgagcga tagtcatgat tcccacaaaa attaatgcag ccccccttcg tgttttcacg
    11101 ccatgttcca tctttttgtt ggaagactac actcgccttt aactgctctt ccaatgttat
    11161 ttcataaggc agatcagcgc tggcaagatc aaattcaaca ttataagctt ctgtagtgat
    11221 gtgagttggg cctatgccgg gtaatttcaa ataatttact tcatccgcaa gtggtcgttt
    11281 actaaataaa gcttcatcaa ggataaccgg ctttggttta ccatattcag cagcaagaga
    11341 gtgaatatag gcatatattt cacttcttac tgcaggatgt acataaccta cagcatcgac
    11401 tcgaacacca tcaaaaccgt actcgatcat gtaacgtttg atataaggtt gccaaaattc
    11461 ggttattatt tcttttctta tgctttcatc atcgtaattg aagccgcgaa catctttgaa
    11521 gtcttcatga actcctttga accaatgagg cttatcctga cacaagggag aatcaatggc
    11581 gacatgattt aaaaccagat caaacattgg aactattccg aggttccttg cattttgagt
    11641 gaataactgt aaagcttctg agtttaatcg ttgggttgtc cccatggggt cttcagggga
    11701 gcccaaactg aattgaggat taaataataa cgggtgactc atggcatata agcttctagt
    11761 gacctcattt cctgttttta caccattatt tttatccgtt ttaaaaaagc cgctaatgtc
    11821 accgggcatt tgcatgggat taatccataa ggcattaaac cccatctgct ttaagacagg
    11881 caaataatta atcatgtcct taatagaatg aaactgagtt gggtacatgt cataacatgc
    11941 aataatccca tttccttctg ttaaaaaatg atatcccata gaatcgatca ccgtagaaaa
    12001 ttattaaact acaaaactga gaaaaatcga attgttggtt acaactccac ttttatcagt
    12061 cgaatcgctt gttattatag cgttaaatag acaaagtagg agctacttca ttttccaatt
    12121 tttcttcttc tggcatatct tttacttcga ctgtgaactc atccaatatc cctttggctt
    12181 tttcagatgc tttatcgtaa attttattaa gcagagtaga ggggattttg cctttggcaa
    12241 tagtttctga tattgcttta tcccaagact ctatctgcgt tttattgcca tgaatcaggc
    12301 ccttgccaaa gtaggcatta ttgatagcac ttatagaata ttggtgctca gataactttc
    12361 gggcaatc
//
LOCUS       DATNSN010000102        12226 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.102, whole genome shotgun sequence.
ACCESSION   DATNSN010000102 DATNSN010000000
VERSION     DATNSN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.102"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..232
                     /locus_tag="VAH76_002157"
     CDS             <1..232
                     /locus_tag="VAH76_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1386763.1"
                     /translation="TFLDMLAELKQSTFKALASLGKTLSAWKDEVARMWRFSKSNGIT
                     EGFHRKMKLIQRRAYGFRNFENYRVRVRVLCG"
     gene            383..2035
                     /gene="kaiC"
                     /locus_tag="VAH76_002158"
     CDS             383..2035
                     /gene="kaiC"
                     /locus_tag="VAH76_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEO1386764.1"
                     /translation="MNIYKTKTGVKGLDDVLNGGYIKNEPTLLKGGPGTGKTIFTLFF
                     THEQLKSNNNVIYITCDESPEKIIAHMDGFNLEGGKFLQEGKFLILDFTPVLSDEVAG
                     EFNINALLLRIEQAQQKIKANTLIIDSLQSILLGIKNYDPNYELLRLFQWARQKNLTV
                     LTTMAETQTILQTEIYDEYVVDCAIELKQKVNNDLMTRYLRIIKNRGSSHGTNEYPFS
                     ITHKGISLLPITSTKLNTTIPAEYLRTGIKVLDRMLDNKGYQTGSQIIISGRSGTAKT
                     LFAASFAQSAVRQGKKVLFISFEESPNDLIRHVKSINIDLMKYITKKKLHINARRPVE
                     MGLEDHIISIIELAEEKQFDVLILDPITSLLDLGNAMEVKRLFIRFISHMKSINKTLF
                     FTELIPDYSKELTILGLSSLTETWIRLSNVENNGEFNRLIHIAKARGIKTSNQIKEFY
                     VTDKGINIEAPYLGDNQMMFGSQKSAHILREKQELQQRKEEIKRLEREITNLTEIQHA
                     QLKIQEANFIAKRSELIAKKNELIAKEEALIKRMESNKLLRE"
     gene            2040..2312
                     /locus_tag="VAH76_002159"
     CDS             2040..2312
                     /locus_tag="VAH76_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEO1386765.1"
                     /translation="MTRTKLKLFVIGNSAISKRAIINLQSICSDPKFADLCDVEVVDL
                     CKNKEIAEQEKILATPILIKKEPLPERRIIGDLSDRQKVIAALEMD"
     gene            2321..4705
                     /locus_tag="VAH76_002160"
     CDS             2321..4705
                     /locus_tag="VAH76_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1386766.1"
                     /translation="MISNYPVITQNFWYQLVEKMAIGVLVINNEGRILYANDYVVQIF
                     GVPSTELVGTNFLYPMAANETQEVEILKSNNEVLTAHMTVKKSIWENRQVWIISLFDI
                     TELKLKEKLLKISSKGISSAFEGIIIIDPEGTIIEANNAFMKMTGYTEKEIIGQKPVL
                     LHSRKQNEKFYKELWNILMAKGRWSGELWAKKKNKRLFPAFLAISAVKNSDGDVINYI
                     GYYHDLTLIKKQEQQIESFKYYDYLTGLPNKLLLTQRLQMLINRLSEENKELIIFNIR
                     IFDSSSKNSLLDFLPNIRDKIILEVVKRIDRTSPKKMFFSRIGLSEFIVVYFIKCRIE
                     VMASIAQRMIEKLSCPYHIGHKTIILQTIIGLTAYYKNTAFSAEDFLQQSEVARHKAQ
                     IKGINIFEFFDPQVEERIIEFNKRIESVSKAIQLNELKIYYQPIVELSKAEVASLEAL
                     LRWKHPKKGLLLPKQFLSDIDNHPLSLQLGDWVLHQVLEQIEKLLACGIKIPIHINVS
                     AYQLQHPNFVKKLFDTLALYPSVPPHLIMLEVLETEAIEDLILVSQIIAECKTKGVNF
                     SLDDFGTGYASLTFLKELNTTEVKIDRSFIIDIFDNPKELVLLKTMIEFCRVIGRTLV
                     AEGVETVAHGKLLSLFGCNLIQGYAIAKPIPATKLIPWIKNWKLAPEWGENRFSHAAL
                     NKLVKISIKHYLNFQLIRLYLENDQSLKPELSLQACPIEQWFLENQQTIKEDRRFKKV
                     CDLHHKEHQLGNIIIQLIQTGQKEQAAKQLVSLDSLRINFLKELMHFICNSNTI"
     gene            5077..5787
                     /locus_tag="VAH76_002161"
     CDS             5077..5787
                     /locus_tag="VAH76_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386767.1"
                     /translation="MRNLKLSHPFSIRISLFLFILLFVPFLSYGAETSNMRGKRYCEI
                     ILAKTISSYAVYNTWGLNDCPEQLWSKVSISAVKKETGSSFVHLNGPRYWVIDGFKNT
                     SLINPAIKTISGIPMREAGILHLSLMDLFKNKPYQSHVVDRKTTWVYQADKPVFELID
                     PNGQVFVMQSYSVQKYPQTMNTLTQLGAKLQLPKGWKFKTGVLNKPETIQAVNNKAVV
                     VQDNFLNTYQKASHDFLK"
     gene            6003..6641
                     /gene="ravN"
                     /locus_tag="VAH76_002162"
     CDS             6003..6641
                     /gene="ravN"
                     /locus_tag="VAH76_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavN"
                     /protein_id="HEO1386768.1"
                     /translation="MPTYFDPIMQEDTVLDENTIVYLVKIGDNKFSIKAISSGLEHLP
                     SDPTTHAEKYWPIPAKSLIDHSSNKLLFEEDKLTNQPISKGQVIELFAVDPDKTEPKQ
                     FSDSVKRELTENWAREVLQDQFAHAGQNPHFFFYQPLFIHGHHGDNPAQQQDNAMPIP
                     LPLLMFSLIPPMNDNNQQQMTLLFFNILLVPQDQGHEPGQENEGEHAPSNGH"
     gene            complement(6735..7430)
                     /locus_tag="VAH76_002163"
     CDS             complement(6735..7430)
                     /locus_tag="VAH76_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386769.1"
                     /translation="MARFFKDREIEIKDSAGEYYKKLCNALKTTMSVCIASTSALVIN
                     ELTAKINKDRMTQYEENSSVAIHGIGEIRVFLLRIYKHLEDNKIDFLDGRAAIKNTMS
                     KGMAGNCEHQAFYLAALLRQQNIPALIYDIEDIGHTVVITQNYLLDPWIGEVFALVKT
                     DLGKFFGSSLNMKASWLNRLLSNKKFSYPDELTLQTLNEYFVQQEDQYRIGLNILDNK
                     EGMDHQMSCELAY"
     gene            7563..7697
                     /locus_tag="VAH76_002164"
     CDS             7563..7697
                     /locus_tag="VAH76_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEO1386770.1"
                     /translation="MEFKMTKYRDAGTGRYVTKEFAKKHPATTVGENSKPKSQRKHKK
                     "
     gene            7990..10050
                     /locus_tag="VAH76_002165"
     CDS             7990..10050
                     /locus_tag="VAH76_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386771.1"
                     /translation="MKFFLKQSRQHSSNQKKRNQPAQSEDHHLIIRQAQFGTIPIDFF
                     GSLRLIFTFSLFCFRAIFGLLTGVVSKKELFDFVFNRTKIGLRISIDTITVRRKVTND
                     TRQIQETTPKKSLLEKESEEPSILTNKNPTIKPNTTHTQNAFFTKSEEKSKTKSFKAV
                     EFHDFNNSEELVAFMGKINELIAQDEAMAIPIKEDENLSSAGEEYIEKLIEIMKSSEL
                     LKHEFWNKLNQMVHKDIQLFVSKLSPAKEKGKFGSSDLDYLWRYFNGFEFEPSKRENR
                     KDKFAVMLEALSEEQLKASFDSPDFLSLLNKDYYIETAANTLKRKQLAFFAANINRHD
                     ILNLMIKKLKPSAYLLGKLVSVMPYATTKIKIALIDQIAHLPHSASFKIELAKLAEHY
                     ISMNTQACIKRYQSLPALPSNTIHTKPPLSKWSSVTAIPKPTYTNISVAKEEWTDNAF
                     EAFLSELNATTYIINEKKIIEDLNSLPDHRIKMIAERASLPDYKDMLKHFWMIESKTI
                     NHRSFIENRKNRLKIILENLSESQLKNSIEDNKFWDIFRNTQEPYCKMAAKALSPKQF
                     EIIITNATLERHSDFAVIISQIKKDSSADKERLQKIIEAIIPHTTPWFALALERQIKG
                     LLTSDKSLFEKFNTDLKKYLSKSLERPEVSLKYRRDRNLNRHAISHLLTEPLENSYKA
                     SFSL"
     gene            10291..11172
                     /locus_tag="VAH76_002166"
     CDS             10291..11172
                     /locus_tag="VAH76_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1386772.1"
                     /translation="MLKRVLNTAKAKQPISTTGIFAHTYYWLTSPAGDQSYQNPNYQN
                     EEDNNTGTAIYFIHGTADQPAAFKRVAERLIDAGLPDEICSLNLLAFDQRYQGKSIKF
                     FAEQLKNKIKANQHQRVILMAHSRGGLVASYFAEFLAKEASIEVPLVITVGTPFNGSY
                     LAIKPLSWFSDSIREMEIDSEFLAQLKQEIVEHSISAYHFFIAKEDAIVPGESGYIKE
                     YVDKYPDSLHILDRHGHLSIMSSHRLVSHIAGLLHDHMDHCETHAEEVKISKLGELTL
                     IEDYYSVKSEPAPSVNP"
     gene            11358..11777
                     /locus_tag="VAH76_002167"
     CDS             11358..11777
                     /locus_tag="VAH76_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO1386773.1"
                     /translation="MNFSAWYFPSLAALILYGAWGYWGTRASDFINPLSITFYSSIGV
                     LISGIIALILLGFKPELSVKGSTYGLLNGLANGIACIFFILALRNGPTMPVVLVTSMY
                     PMITLIFCMIFLKQELSLKQGLGMVFALIALVLFSTE"
BASE COUNT         4182 a         2293 c         2129 g         3622 t
ORIGIN      
        1 tacattcttg gatatgctag ctgaattaaa acagagcacc tttaaagcgt tagcatcatt
       61 aggaaaaacg cttagtgctt ggaaagatga agtcgccaga atgtggcggt ttagtaaatc
      121 taatggaata actgaaggct ttcatcgcaa aatgaaactc attcagcgaa gggcttatgg
      181 ttttagaaat tttgaaaatt atagagtgcg tgttagggtg ctctgcgggt gattaaagac
      241 tgccccctta aatgggaaag agccaatttt ttacggctct atcaagacga aatattagat
      301 ctataattag acagtcagca tattttttta tcacctcaac agtggtattt taaagatatg
      361 catccgttga gaggacgaaa atgtgaatat ctataaaaca aaaacgggag ttaagggatt
      421 agatgatgtt ttaaatggtg gttatataaa gaacgaacca acccttctca aaggcggccc
      481 tggcacagga aaaacaatat ttaccctttt ttttacacat gaacaactta agtcaaacaa
      541 caacgtcatt tatatcacct gtgatgaatc gccagagaaa attattgctc atatggatgg
      601 ctttaacctg gaaggcggca agtttttgca agaaggcaag tttttaattc ttgattttac
      661 acctgtattg agtgatgaag tagctgggga gttcaacatc aatgcgcttt tactccgtat
      721 tgagcaggca caacagaaaa tcaaagccaa tacactaatt attgactcac ttcaatccat
      781 ccttttaggc atcaaaaatt atgatcccaa ctacgagttg ttgagattgt ttcagtgggc
      841 gcggcaaaaa aatcttaccg ttctcacaac gatggccgaa acccaaacga ttttacaaac
      901 agaaatctat gacgaatatg ttgttgattg tgctattgag ctaaaacaaa aagtcaataa
      961 cgacctgatg acgcgctatt tgcgaatcat taaaaatcgt ggttcgtctc atgggactaa
     1021 tgagtatccc ttttctatta ctcataaggg catttccttg cttcccataa ccagcacaaa
     1081 actgaataca acaattcccg ctgaatatct ccgcactggc ataaaagtcc tggataggat
     1141 gttagacaat aaagggtacc aaacaggatc gcaaataata atcagcggac gttcaggaac
     1201 agcaaaaacg ctttttgcag caagtttcgc ccaatccgct gttcgccaag gaaagaaagt
     1261 gctgttcatc tcatttgaag aatccccaaa tgatttaatc cgtcatgtca agtccattaa
     1321 tattgattta atgaaataca tcaccaaaaa aaaattacat attaatgcaa gacgccctgt
     1381 ggaaatgggg ctggaagatc atattatttc aatcattgaa ttagcagaag aaaagcaatt
     1441 tgatgtcctg atactggatc ccatcacatc tctgctggat ttgggtaatg cgatggaggt
     1501 caagaggttg ttcatacgtt tcatttctca tatgaaatct atcaataaaa ctcttttttt
     1561 tactgaactg atacccgatt attccaaaga gctaaccata ctgggcttat ccagcttaac
     1621 agaaacctgg attcgcttat ccaatgtgga aaacaatgga gaattcaatc gattaatcca
     1681 cattgccaag gcgcgtggca taaaaacatc gaaccaaatc aaagaatttt atgtcacgga
     1741 taaaggtatt aacatcgaag ccccttatct cggtgataac cagatgatgt ttggttctca
     1801 aaaatcagcc cacatcttga gagaaaaaca agaattacaa cagaggaaag aagaaattaa
     1861 acgcctagaa cgtgaaatca ctaatcttac agaaatacaa catgctcagc tcaaaattca
     1921 agaagcaaac tttatagcaa aaagaagtga gttgattgct aaaaaaaacg aactgattgc
     1981 taaagaagaa gcgctgatta aacgaatgga gtccaacaaa ttgttaagag agtaaatgaa
     2041 tgactcgaac taagctaaaa ctgtttgtga ttggaaatag tgctatatca aaacgtgcca
     2101 ttattaattt gcaatcgata tgctcagatc ccaagtttgc agatttgtgc gatgtcgagg
     2161 tagtcgatct atgtaaaaac aaggagatag cagaacaaga aaaaattctg gcaactccta
     2221 tactaattaa aaaagaaccc ttgcctgaac gtcgaatcat cggtgattta tctgatagac
     2281 aaaaagttat tgctgctctg gaaatggatt aggtgatatc gtgatttcaa actaccctgt
     2341 aatcactcaa aacttctggt atcaactggt tgaaaaaatg gctatagggg tgttggtgat
     2401 taataatgaa gggcgaattc tttatgcgaa tgattatgta gtgcaaatct ttggtgtacc
     2461 cagcacagaa cttgttggca ccaatttttt atatcctatg gctgcaaatg aaacacagga
     2521 agttgaaatt ttaaaatcca ataatgaagt tttaacagct catatgacag tcaagaaaag
     2581 tatctgggaa aatcgccagg tatggattat ttctctcttt gatattacag agctcaaatt
     2641 aaaagaaaaa cttttgaaaa tttcaagcaa aggcatcagt tcagcctttg aaggcattat
     2701 catcatcgat cccgaaggca cgattattga agccaataac gcgtttatga aaatgaccgg
     2761 atatacagaa aaagaaatca taggacaaaa acctgttctc ttgcattcaa ggaagcaaaa
     2821 cgagaaattt tacaaggagc tatggaatat actcatggcg aagggtcggt ggagcggtga
     2881 actttgggca aaaaagaaaa ataaaaggtt atttcctgcc tttttagcca tttcggctgt
     2941 taaaaactcc gatggtgacg tgattaatta cattggttat tatcacgatt taacgttaat
     3001 aaaaaaacaa gaacaacaaa tcgagagttt caaatattat gactatttaa caggactacc
     3061 gaacaaactt ttactcaccc aaagacttca gatgctcatt aacagattat ccgaggaaaa
     3121 taaagaactc atcattttta atatcaggat atttgattcg agtagcaaaa actctctgtt
     3181 agattttctt ccaaatatcc gagataaaat catcttggag gtggttaagc gtattgaccg
     3241 aacatcacct aaaaaaatgt ttttctcacg catcggcttg agtgagttta ttgttgttta
     3301 ttttatcaaa tgtagaattg aagtaatggc ttccatcgcc cagagaatga tagaaaaact
     3361 ctcttgccct tatcacattg gtcacaaaac tattatcttg caaacgatca ttggattaac
     3421 tgcgtactac aaaaataccg cgttcagtgc cgaggatttt ttgcaacaat cagaagttgc
     3481 ccgccacaaa gcccaaatca agggtattaa tatttttgag ttttttgacc ctcaggttga
     3541 agagcgaatt atagaattta ataaacgcat agaatctgtc tctaaagcaa tccagttaaa
     3601 tgagttgaaa atttactacc aacctattgt ggaattaagt aaagctgaag tggcaagttt
     3661 ggaggctttg ctgcgctgga aacaccccaa aaaaggcctg ttacttccaa aacaattttt
     3721 atctgacatc gataatcacc ctctttctct gcaacttgga gattgggttc tgcatcaggt
     3781 attagagcaa attgaaaaac tactggcatg cggcataaaa attcccattc acattaatgt
     3841 gagcgcttat cagctgcaac atcccaattt tgtcaaaaaa ctgtttgaca cattggcact
     3901 atacccaagc gttccccctc acctaatcat gcttgaagtc cttgaaacag aagccattga
     3961 agatttgata ttagtatctc agataatagc cgaatgtaag accaagggag tcaacttttc
     4021 tttggatgat tttggaacag gttatgcctc tttaactttt cttaaagaat tgaatacaac
     4081 tgaagttaaa attgatcgaa gttttatcat tgatattttt gataacccga aggaacttgt
     4141 tcttctgaaa acaatgatag aattttgccg agtcattggt cgtaccctgg ttgcagaagg
     4201 ggttgaaaca gtagcacatg ggaaattatt aagcttgttt ggttgcaatc taatccaagg
     4261 ctacgctatt gccaaaccta tccccgctac caaattaatt ccatggataa aaaactggaa
     4321 gcttgctcca gaatggggag aaaaccggtt ttcgcatgcc gcattaaata aattagtcaa
     4381 aatctccata aaacactatc ttaactttca attaatcaga ctttatttag agaatgatca
     4441 atctctcaaa cctgaattat cacttcaagc atgcccaata gagcagtggt ttcttgaaaa
     4501 ccaacaaaca atcaaagagg acaggcgatt taaaaaagtc tgtgatttac atcacaagga
     4561 acatcaattg ggcaatataa taattcagtt aattcaaaca gggcaaaaag aacaagcggc
     4621 caaacaactg gtaagccttg atagtttaag aattaatttc ttgaaagagt taatgcattt
     4681 catttgtaat tcaaatacca tttaattgat tacataatgc acttcaatta aaacgcctga
     4741 ttacatccaa gagaagcctc aagattttct taatttcaac tttagccact ttttcgtaaa
     4801 ctgctcatcc tgaggagcgt gcgcagcacg cgtctcgaag gactagttag gcagccaaaa
     4861 agatataata aatcaagata ttaattattt gctatgggat ggacagctca cgaaaaagtg
     4921 gctaaagtcg agttgatgat ccatgactcc caactcccaa aatggccatt gaagcttatt
     4981 gaactacagc ctatttgagc atttcccaat tttgtagata gaatagtctt tgtaagttaa
     5041 agtgcaaata gatataaaaa tgacaaggag gccgtcatgc gcaatttaaa actttcgcat
     5101 cctttttcta taaggatttc tttattttta ttcattttac tctttgttcc cttcctatct
     5161 tatggcgctg aaacatccaa catgcgtggc aagcgatatt gtgaaatcat cctggcaaaa
     5221 accatatcca gttatgccgt gtataatact tggggattaa atgattgccc ggaacaattg
     5281 tggagtaaag tttctatttc cgcagtaaaa aaagagactg gctcttcttt tgttcatctc
     5341 aatggccctc gatattgggt aattgacgga ttcaaaaaca cttctttgat aaatccagcc
     5401 atcaaaacaa ttagtggaat tccaatgcgt gaagcaggca ttttgcacct tagtttaatg
     5461 gatttattta aaaataaacc ctatcaatca catgtcgtgg accgcaaaac aacatgggtc
     5521 tatcaagcag ataaacctgt ctttgaattg attgatccca acggacaagt ttttgtaatg
     5581 caatcctata gtgttcaaaa atacccacaa acaatgaata ccctcacaca actaggcgcc
     5641 aaacttcagc ttcccaaagg ttggaaattt aaaactggtg tgcttaacaa gccagaaaca
     5701 attcaagctg ttaataataa agccgtagtt gtccaagata attttttgaa cacctatcaa
     5761 aaagccagtc atgattttct gaaataaatt tagcatgcgg aaagagcgtc acagggcaaa
     5821 agattaaatc ttgccctgtg cctaaattga gaactgacaa gtgtcagtca ttgtatttaa
     5881 tccaaactgt ttgcaaacca aatcatattg cttgattaaa taatgaatca aaagtattgc
     5941 aaaagccaat ttgatcctat aagttactat tgagtttaaa taacagctaa aggactatta
     6001 ttatgccaac ttattttgat cctattatgc aagaagatac tgtgctggat gaaaacacga
     6061 tcgtttactt agttaagatt ggtgataata aattcagtat taaagcaatt tcatcaggat
     6121 tggagcatct ccctagtgat ccaactactc acgcggagaa atattggcct attcctgcca
     6181 aatcattgat tgatcattca agtaacaaac tcctttttga agaggataag ttaaccaatc
     6241 aacccataag caaaggtcaa gtaatagaac tttttgcagt tgatcccgat aagactgaac
     6301 ccaagcagtt tagtgactcc gttaagagag agttaactga aaactgggca cgagaggtac
     6361 tacaggatca atttgctcat gcagggcaaa accctcattt ctttttttat caacccctgt
     6421 tcatccatgg gcatcatgga gataaccctg ctcaacaaca ggataatgcg atgccaattc
     6481 ctctcccact cctgatgttt tccttgatac cacccatgaa tgataataac caacaacaaa
     6541 tgacattatt attttttaac atactcctgg taccacagga tcaaggccat gaacctgggc
     6601 aggaaaatga aggcgagcat gctccttcta atgggcactg acattaaacc taacttgcat
     6661 ttctaacaag attaagtcgg gcaccgcgcc cgacatttct attttgacag gtgcgctcag
     6721 gcggcaatgc ttttttaata agctagttcg caagacattt gatgatccat accctcttta
     6781 ttatccaaaa tattaagtcc gattcgatat tggtcttctt gttgtacaaa atattcgttt
     6841 aatgtttgga gagtaagttc atcagggtag ctgaattttt tattacttaa taatcgattt
     6901 agccacgagg ctttcatatt cagtgacgac ccgaaaaatt tgccgagatc agttttgaca
     6961 agtgcaaaaa cctcacctat ccatggatca aggagataat tttgagtgat aactacagta
     7021 tggccaatgt cttcaatatc ataaataagc gcagggatat tttgttgtct cagcaaggca
     7081 gcaagataaa aagcctggtg ttcgcaattt cctgccattc ccttcgacat tgtgtttttt
     7141 attgccgccc tcccatctag aaaatcaatc ttgttgtcct caagatgttt atatatcctg
     7201 agtagaaaaa ctcttatctc accgatacca tgaatagcaa cagagctatt ttcctcatat
     7261 tgagtcattc tatctttatt gatttttgca gtgagttcat taataactaa tgcagaagtg
     7321 ctggcaatgc acactgacat ggttgttttt agagcattac acagtttttt gtaatactcg
     7381 cctgctgaat ctttgatttc aatttccctg tctttgaaaa atctggccat agctaatccc
     7441 ttatatctaa taagggtttt agtttacata caagtatgag aggagcaagt agaacagcct
     7501 ctttttcacc aaaactcctc ttgcgaactg aatttgctat acttccattg gagcggataa
     7561 aaatggagtt taaaatgaca aaatatagag atgcaggaac cggtcgatac gtcaccaaag
     7621 agtttgcaaa gaaacatccc gcaacaaccg ttggtgaaaa tagcaaacca aaatcacaaa
     7681 gaaaacataa aaaataatcg ccatggagta attggccttt ggttgcgttt gacgcaagag
     7741 actaatcatg ttgtcctaaa aataggccat aaagagagtt tcttgatatt ttaaatcata
     7801 aaaggtgaag aattttcata aagcagaact tgtatatcgc atttaaacat tgatgtttta
     7861 acataaatcc agcaatataa atcttttgta cgaattcaac tcttttaacg aattctcagg
     7921 atgatttagt taataaaata aaccattact tttacattac atttaaccat atggatatta
     7981 gttaatataa tgaaattttt tttaaaacaa tctcgccagc attcctctaa tcaaaaaaaa
     8041 agaaaccagc ctgctcaatc tgaagaccac catttaataa ttcgtcaagc tcaatttggc
     8101 actatcccta ttgatttttt tggcagccta aggcttatat tcaccttttc gttattttgc
     8161 tttagagcaa tttttggttt gcttactggc gttgtttcta aaaaagagct ttttgatttt
     8221 gtatttaata gaacaaaaat tggccttaga atttcgattg ataccattac agtgagaaga
     8281 aaagtaacaa acgacactcg gcagatacaa gaaacaacac caaaaaaatc attattagaa
     8341 aaagagtcag aagaaccatc tattttgacc aacaagaatc cgaccatcaa gcccaatact
     8401 actcatacgc agaatgcttt tttcacaaaa tctgaagaga aatcaaaaac aaaatcattt
     8461 aaagcggtcg aatttcatga tttcaacaat tctgaagagc ttgtggcttt tatgggaaaa
     8521 ataaatgagc ttatagcaca agacgaggca atggctatcc cgataaaaga ggatgagaac
     8581 ctgtcttcgg caggggaaga gtatattgaa aaattaattg agataatgaa aagctctgag
     8641 ttactaaaac atgagttttg gaataagctg aaccagatgg ttcataaaga cattcaatta
     8701 tttgtcagta aacttagccc agctaaagaa aaagggaaat ttggctcaag tgacctggat
     8761 tatttatggc gttattttaa tggttttgaa tttgagcctt caaaaagaga aaatagaaaa
     8821 gataagtttg cagtgatgct tgaagccctt tcagaagaac agttaaaagc gtcttttgat
     8881 tcacctgatt ttttaagcct tttaaataaa gactactaca ttgagactgc agctaacaca
     8941 ttaaaacgta aacaattggc attctttgct gcaaatatca acaggcacga cattctgaat
     9001 ttaatgataa aaaaactaaa accaagcgct tatttgttag gcaaacttgt ttctgttatg
     9061 ccttatgcca ctacaaaaat taaaatagcc ctgatcgatc agattgccca tttacctcat
     9121 tccgcttcgt tcaagattga actcgcgaaa ttggctgaac attatattag tatgaatacc
     9181 caagcatgta taaaacgtta ccagtcccta cccgctctcc catccaatac aatccacact
     9241 aaacctcctc tctcaaaatg gtcttctgta actgctatcc ccaaaccaac ctataccaat
     9301 atatctgtag caaaagaaga gtggactgat aatgcgtttg aagcattcct gagtgaactg
     9361 aatgctacaa cgtacattat caatgagaaa aagataattg aagatttgaa tagtttgcca
     9421 gatcatagaa ttaaaatgat agcagaaaga gcttctttac ccgattataa ggatatgcta
     9481 aaacactttt ggatgattga atccaagaca ataaatcata gatctttcat agagaacaga
     9541 aaaaatcgcc tgaaaattat tctagaaaat ttatcggaat ctcaattaaa aaattccata
     9601 gaagacaaca agttttggga catttttaga aacactcaag agccttattg caaaatggct
     9661 gccaaagcat tatcccccaa acaatttgaa ataatcatta ctaatgccac actcgaaagg
     9721 cattctgatt ttgcagtaat catttctcaa attaaaaaag acagcagcgc tgacaaagaa
     9781 agactgcaaa aaattataga agcaatcatt ccccacacta ctccatggtt tgctttagct
     9841 ttagaacgcc aaataaaagg tttattgaca agtgataaat ccttgttcga gaaatttaac
     9901 actgatttaa aaaaatacct cagtaaatcg ctggaaagac ccgaagttag tttaaaatac
     9961 agaagagaca gaaatctaaa tcgacatgct atttctcatc tcctgacaga accacttgaa
    10021 aactcttaca aagcgtcctt tagcctttaa agatactcta ctactacctt aagaactctc
    10081 tgcttttgac cggcaaggca ccaattataa aaaccaatag agacgttaac caaatcacaa
    10141 ttcattcata atccattgac aataactgac cccgataacg ctttaaagca cagtttataa
    10201 ttgactcata ttaagctata ttattgagaa attattgaca gtatccgact tccggaactc
    10261 gacgctccac aataaaaaag gaatttaact atgttgaagc gcgtactcaa tactgcaaaa
    10321 gccaaacaac ccatttccac cacgggtata tttgctcata cttattattg gctaacttct
    10381 cctgctggcg atcaatccta tcaaaatcct aattaccaaa acgaagaaga caacaacaca
    10441 gggacggcaa tctattttat ccatggtaca gctgatcaac cagcggcatt taagcgtgtt
    10501 gctgaacgtt tgatcgatgc cggtcttcct gatgaaattt gctctctgaa tttactagcc
    10561 tttgatcagc gttaccaggg aaaaagtatc aaattttttg ctgaacaact caaaaataaa
    10621 ataaaagcta atcagcatca gcgagtcatt ttaatggcgc actctagagg agggctagtt
    10681 gcttcttatt ttgcagagtt tttagcaaaa gaagcctcca ttgaggtacc gctagttatt
    10741 acagtgggca ccccgtttaa tggttcttac cttgctataa aaccattgtc atggttttct
    10801 gattctatta gagaaatgga gattgatagt gaatttcttg cgcaacttaa acaagagata
    10861 gtagaacact ccattagtgc gtaccatttt tttattgcaa aagaagatgc gattgtacct
    10921 ggagaatctg ggtatataaa agaatacgtt gataaatacc cagattcgct gcatatttta
    10981 gatcgccatg gccacctttc cataatgtcc tctcatcgtt tggtatccca tattgccggt
    11041 ttactccacg atcatatgga tcactgtgaa actcatgcag aagaagtaaa aatttctaaa
    11101 ttaggggaac tcacactaat tgaagactat tattctgtaa aatctgaacc agccccctct
    11161 gtcaatccat gaaacatcca ttcatggatt ttttggttat tatttattca gaaaataaac
    11221 tatattggta attagcattt ttataaacca cagctcatcg tgataataag taaaatttag
    11281 taataaacag tattattttc actaatgaac agttatcctg agcaaacctc aaacaaattg
    11341 cttgataaga taaaattatg aattttagcg cttggtactt cccttcttta gctgcattaa
    11401 ttctgtatgg agcttggggg tattggggaa caagggcctc tgattttatt aatcccctat
    11461 ccattacctt ttattcaagt ataggggttt taatctcagg aattattgct ttgattttat
    11521 tgggtttcaa acccgaacta tctgttaaag ggagtactta tggcttgctc aatgggctgg
    11581 ctaatggaat tgcctgtatt ttttttattt tggcattacg taatggcccc accatgccag
    11641 tagttttggt cacctctatg tatcccatga ttactttaat attctgcatg atttttctca
    11701 aacaagaatt aagtttaaaa caaggattgg ggatggtttt cgcattgatt gccttagttt
    11761 tattttcaac tgaatgaaaa ctccaatcag atgaaatagc cctaaatttc tttggattat
    11821 caaaattaaa gagccttgct ccaaaactta aaaatttggg tcacgaagct tgactctcaa
    11881 attggtagtt tgttcaaaaa atcttgaatt caactaaact gtaagataaa ccatcatatc
    11941 attaataata attaagtaag gacaagcaag cgtggcaata aatagagtta ccaggcaaaa
    12001 attttcttta ctaattaaca aagcgtggtt tttgccttta atgatatcaa tagctattaa
    12061 tctaactggt attaccctga ttttactgag taaaaatatt aatggattct atctctcaat
    12121 atctacggag agtcaacaaa caattatcag catcacttca ggctctttaa tgactttaat
    12181 tggtctgact ttctcaatca gcatgctgat tctatctacg gtatca
//
LOCUS       DATNSN010000103        12142 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.103, whole genome shotgun sequence.
ACCESSION   DATNSN010000103 DATNSN010000000
VERSION     DATNSN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.103"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            566..1186
                     /locus_tag="VAH76_002168"
     CDS             566..1186
                     /locus_tag="VAH76_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386774.1"
                     /translation="MSHYGQSEVQYVKVDADNLRYISGGVPGIPGKYQFKPTDEVNVK
                     TPKEWLAKIGFDSKIEALQNKVTMLGKEDDRASEDLSFIIGNLITYKQEFLDGHKSAE
                     QFVEACRDEIKPEKTKNLANHRGIFASVTSFIESIVQLVSPKYKINTDSINKINEVEK
                     SLGEIKPERVVKETGIAKRTIKSDYEVDSNNTVAKAGDRRPGIKPG"
     gene            1455..1832
                     /locus_tag="VAH76_002169"
     CDS             1455..1832
                     /locus_tag="VAH76_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO1386775.1"
                     /translation="MRDPEISLLDHLPGYIGWKDLNRHYVGANKALLELKGFRHVEEL
                     AGKTDEELSPWAIEENKLFQQQDFHVINGEKVATVHFNAKTSEVFLLEKCSLTDQNNQ
                     VTGLIYHYHIPARLKQVAVIKSL"
     gene            1937..2920
                     /locus_tag="VAH76_002170"
     CDS             1937..2920
                     /locus_tag="VAH76_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEO1386776.1"
                     /translation="MRQLFIIMFVFLLSNVGYGAPAFEILPLGVYGGLKDGNLSAYLI
                     KAIESKNYTALDGGTLVRGLEVAVDKQSLSNKRVDDLLQKHIPAYLISHAHLDHVMGL
                     VMAQPELREKQTIMAREETMQALQKNIFNWSVWGNFGDSGDTPQLNFQHYQTVPLFQW
                     VSIPDTDLHVKVFPLSHGGMASSAFLIRYEAEYVLYFGDTGADSIEKSTNMENIWKEI
                     APLIRNRQLHAIMLECSFLNSQPDDKLFGHLKPNLFMSELRQLASIVDPFDSRNSLRG
                     LPVVVTHIKPRLADFSNTNEDTEKQIINELSQENDIGVELIKPEQGITLSL"
     gene            complement(3102..4106)
                     /locus_tag="VAH76_002171"
     CDS             complement(3102..4106)
                     /locus_tag="VAH76_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1386777.1"
                     /translation="MKQTAIVLQGGGALGAYELGVLKYLYASDSFSPNIISGVSIGAI
                     NAATLIGAKDDPIQTLEAIWDELTVFSAQMFPENAEPYLSLFGNAAFYHLRTDYWSMP
                     YWTNFYTTTPLQQLLLKYIDFDKLNNSPIHLILTATDVTTGKSTVFENQGNNRTEITP
                     LHVLASGSLPPGFPMTLIGNTHYWDGGLFSNTPLSPVMEKLNPDPNVEKQIIVINLFP
                     SQGKIPTNMPEVFDRVFEIIFSNKIRFNTELTEKINEYIETIQEIEKVIPENSFIRQL
                     PGYKRLLNYKYIQDIIYIESSDEENATASFDFSRKAIQRRIDSGYKDAKNNFQLNQLI
                     "
     gene            4501..4740
                     /locus_tag="VAH76_002172"
     CDS             4501..4740
                     /locus_tag="VAH76_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1386778.1"
                     /translation="MEVDHIKNEVKLFLLNSFRIKDIGYSDNIFDTGAMHSLFFIQLL
                     VFIEKKYKIDLEVGEFDLNQLTSIDAIAHFISSKL"
     gene            4849..5703
                     /locus_tag="VAH76_002173"
     CDS             4849..5703
                     /locus_tag="VAH76_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEO1386779.1"
                     /translation="MKQTKLTLLGAGTMGSGITQLFAQYGFYVTLIDNLQSQLDKAKD
                     TIAKNLHYLALTQNLESTHSIETILASITFTTKLDELKQSEYIIENITENWERKKALY
                     QVLKKECSATCILGVNTSSIPITKIASLVDHPQRVIGVHFMNPAPMMPMVEVIKGYHT
                     DELTIEKTRTLLEQVHKKMIVVKDSVGFVSNRAMMIFINEAIFMVQENIASIEDIDVL
                     FKQCFGHKMGPLHTADLIGLDTVLYSLETIYSELNDPKYRPCWLLRNMVDAGLLGQKT
                     KKGFYQYE"
     gene            5773..6900
                     /locus_tag="VAH76_002174"
     CDS             5773..6900
                     /locus_tag="VAH76_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEO1386780.1"
                     /translation="MPNYQPQYVKQQRLFNQFIENCLHSHAKIFDKEQNIPRDFFYTL
                     AQNGFLGASIPKQFGGQQWDELTIGIMHESFAKELCSLANILTVLGMVSKSLLQFGSK
                     AQKQIWLPRIASGETLAALALTESNIGSDLEHVETQLLDAGDEFILKGSKKYITLGQI
                     ADLFLVLANCQGQLTAVLVEKDTSGLIITPMSNFLGLRSNMLAEIFFDNCRIPKTNLL
                     GSIGIGFNCVIQTALDEGRYTTAYGCVGLGQACLDAALGYTQKRKQFDRRLDEHQLIQ
                     KMITEMVAQVKAARELCFYAGELRQQKNPAYIAETLIAKYAASKMVVFVSNHALQIFG
                     AAGFEETYPVERYYRDAKVMEIIEGTSQMHEILISKLCNNK"
     gene            6917..8440
                     /locus_tag="VAH76_002175"
     CDS             6917..8440
                     /locus_tag="VAH76_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEO1386781.1"
                     /translation="MGSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQVN
                     QYARYLQRKGLPLNGIVGIQLTRSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILE
                     DSQIKFLIVSDHSLLTATPGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTG
                     KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIAGAGVVL
                     LPPRKEHDIKLFIQMIEQYQINVIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGE
                     ALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQ
                     FFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIV
                     RWNQEGELLFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMV
                     VFLIANNKQTKIGSAELKSFLKNRLPDYMIPEHYLWLSAFPIKENGKIDKEKLLSFI"
     gene            8610..9926
                     /locus_tag="VAH76_002176"
     CDS             8610..9926
                     /locus_tag="VAH76_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEO1386782.1"
                     /translation="MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSE
                     EQLHRSGISEQLIANPYYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTY
                     RALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIA
                     PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYL
                     YEENGLFSSDGHCHSFDVSACGTLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNN
                     DGNSSKEGFMAPGIHGQYTCLQSAWKNAGIEPGDLDYIAAHGASTKLGDATEIYALKK
                     AFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFM
                     LEDSPIYIATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN"
     gene            complement(10239..11720)
                     /locus_tag="VAH76_002177"
     CDS             complement(10239..11720)
                     /locus_tag="VAH76_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEO1386783.1"
                     /translation="MSFFDLLINKANHLVNQIYKKNYNSITSDQLIWQHIQDCEKQFQ
                     KNQEAIYYSKDILQDLINKRLQTILLHAKTKSPWYKKTLAKINIENFTRERLEELPTI
                     NKTILMENWDAIVTDPKLSLNLVEKHLSKKNDSIDTLYLFSRYQVVSTGGSSGKRGVF
                     IYDWDEWITFHTAFVRYPLYNYERTQIVTTKLNTNPRIVSLFVTNTAIAAYSLAKTFG
                     RGNQNMYFLPMAVTPLNIVITRLNQIQPDILSAGPSYIHKVCQLVQKGQIKIEPKILF
                     VGGEPLFEQTLALIKETWPQVDIFNVFGCTEGMVGLNCRANVDEMHLNDDQCIIEPLD
                     EQNRPVDKGIMVSKMYITNLYNYTLPLIRYENSDEILFLNKTCDCGIHHQLIQTPKGR
                     PGCDFNYPGNIFVHHSLFLGALLPEKNIQEYQVEQTIDGVNIKVVTSGYINKSELQKN
                     IRLRLSKVGLVDAQVNITEVPEIKYLYSGKLNRFIPLNSADQS"
BASE COUNT         3826 a         2139 c         2448 g         3729 t
ORIGIN      
        1 aaaagatata agtgtatgtt tttcacgttt aaaaaatatt tcaagatcct ctggttaata
       61 atatttttaa gtgtttttat tttaaccaat taatctggat ttttaattaa cagatatgtt
      121 tgattttcga cataatgcgg ggtttttcat tcagaattat aaagtttatg tgccctatcc
      181 ctacctctcg tggagcacag agtttaggat aaagaacttt gagttggtta ttttttatac
      241 taaacttaca ctagataagt tcactggagg ctattgtatg gaaccgttag aaacgaatgt
      301 tacctatttc gtgataggta aaccaattac aaccatagaa tctaattcca aacaaagaaa
      361 aaaaatttct gatgatgaac taaaagaggc tgtcccgaca gggcacgctt tgctgtttcg
      421 tacacgagag gaagctgatg aatatgcgag acttacggaa ggatataata gcaaagtacc
      481 ggactgtcct aggtacaaag acgctgtatt tgaggttcag cttaggaata ctattgatga
      541 acctcaagtg gttacaaaaa aaataatgtc acattatggg caatctgaag ttcaatatgt
      601 aaaggtagat gctgataatt tgcgttatat ttcaggcgga gttcctggga ttccgggtaa
      661 atatcaattt aagccaacag atgaggtaaa cgttaaaact ccaaaggaat ggttagctaa
      721 aatcgggttt gacagcaaga ttgaagctct gcagaataaa gtgacaatgc tcggaaaaga
      781 ggatgaccgt gcaagcgagg acctgagttt cattattggt aatttaataa catataaaca
      841 ggaatttctt gatggtcata aatcagctga gcaatttgta gaggcttgta gagatgagat
      901 aaaacctgaa aagacgaaaa acttggcaaa tcaccgtggg atttttgcct cagtcacatc
      961 atttattgaa tctattgttc aattggtaag cccaaaatac aaaattaata cagattccat
     1021 taataaaata aatgaagttg agaaatcatt aggtgaaatc aaaccagaac gagttgttaa
     1081 ggaaacaggt atcgcaaaaa gaacaattaa atctgactac gaagtagata gcaacaatac
     1141 agtggctaaa gctggagata ggagaccagg tattaaacca gggtagttta cagagtggtt
     1201 tcatatgaat tgggctttca taaattcgac gtgctgatag ctattaaaaa agcctgtggt
     1261 taagagccca ttatttcaac tccatcccag agtttagaaa tgacaataaa atgcttacct
     1321 ttctttaaag acaacgattg attagactcg gctctgtgat ataagcagct gattcttcca
     1381 atctgccttg aaaattacgc cccaaccgtt taataatcca tgctcgaaat gagcattcat
     1441 tggaggatgg gctaatgcgg gatcctgaaa tatcattact tgatcatctc ccaggctata
     1501 ttggctggaa ggatttaaac cgccattatg taggcgcaaa caaagcgttg ctggaattga
     1561 aaggatttcg tcatgtagaa gagcttgcgg gtaaaacaga tgaagagctg tcgccgtggg
     1621 ctattgaaga gaataaactg ttccagcagc aagattttca cgtaataaac ggtgaaaaag
     1681 tagcaacggt tcattttaat gcaaagacta gtgaagtgtt tctgttggaa aaatgttctt
     1741 taacagatca gaataatcag gtgacaggat tgatttatca ttatcatatc ccagcaagac
     1801 taaagcaagt ggctgttata aagtcacttt aatcattatg atgttgaact tccaccgtat
     1861 aaatacggtg attgaagcaa ggttgaataa ggtattattt tacaaactga cccgaattat
     1921 ttgaaaggaa atcctcatgc gccagctatt cattatcatg tttgtttttc tattatctaa
     1981 tgtaggatat ggtgcgcctg cctttgaaat tcttcccctt ggagtctatg gtggtttgaa
     2041 agatggaaat ttatcagctt atttaatcaa ggccatcgag agcaaaaatt atacggctct
     2101 tgatgggggc actttggtgc gagggcttga agttgcagtc gataaacagt cactgtctaa
     2161 taaaagggtt gacgatttgt tgcaaaaaca tataccagcc taccttattt ctcatgccca
     2221 tcttgatcat gttatggggc ttgtgatggc tcaacccgaa ttgcgagaaa agcaaaccat
     2281 tatggcacgt gaagaaacca tgcaggcatt gcagaaaaat atttttaatt ggtctgtttg
     2341 ggggaatttt ggagatagtg gtgacacccc tcaattgaat tttcaacatt accaaacggt
     2401 gccattattc cagtgggttt ccattcctga cacggatttg cacgttaaag tgttccctct
     2461 aagccatgga ggaatggcat caagcgcttt tttaatccgt tatgaggctg aatacgtcct
     2521 ctattttggt gatactggtg ctgatagcat tgaaaagtca accaatatgg agaacatttg
     2581 gaaagaaatt gccccactca taagaaatag gcagctgcat gccatcatgt tggagtgctc
     2641 atttttaaat tcacagccag acgacaaatt atttggtcat ctaaaaccta acctattcat
     2701 gtcggaactg cgacaattag catcgattgt tgatcctttc gactcaagaa actcactacg
     2761 aggtttaccc gttgttgtta cacacatcaa acccagatta gctgactttt caaatactaa
     2821 tgaagataca gaaaaacaga ttataaacga attgtcccag gaaaatgata ttggcgtcga
     2881 attaattaaa cctgagcaag gtataacctt aagtttatga ttgatgaaga gtcacaacag
     2941 ctgccagaac caatcctaat aaatcattta tggtatttgt gtatcagggg ctgttgcgaa
     3001 aaatccgaac cagaataaaa aaaccttagc atagagaagt tgctactcct gcaactttgc
     3061 atgctatgac ggcagcaatg taatagtgga tgcggcagag ttcaaattaa ctgattcaac
     3121 tgaaaattat ttttggcatc tttgtatcct gagtcaatgc gcctttgtat cgctttgcgg
     3181 gaaaaatcaa aagaagcggt tgcgttttct tcatctgagc tttctatata aatgatatcc
     3241 tgaatatatt tgtaattaag cagtcgttta tagccaggaa gctgtcttat aaagctattt
     3301 tccggaatca ctttctcaat ttcctgtatt gtttcaatgt actcattaat tttttcggtt
     3361 aattcggtat taaaacggat tttattggaa aaaatgattt caaacactct gtcaaaaact
     3421 tcaggcatat tagtcggtat ttttccctgg ctggggaaaa gattaatcac aataatttgc
     3481 ttttcaacgt ttggatcggg atttagtttt tccataaccg gagatagtgg agtattggaa
     3541 aataagccac cgtcccaata atgagtatta ccaatgagcg tcatgggaaa gccaggaggc
     3601 aaactgccac ttgctaacac atgcaaaggc gtgatttctg tgcggttatt accctgattc
     3661 tcaaaaacag tacttttacc cgttgtaaca tcagtcgctg tcagaattaa atggattgga
     3721 ctgttattca gcttatcaaa atcaatgtat tttaataata attgctgaag tggtgttgtg
     3781 gtataaaaat tggtccaata aggcatagac cagtaatcgg tacgcaaatg ataaaaagca
     3841 gcattgccaa agagtgacag ataaggttcg gcattttcag ggaacatctg tgcagaaaaa
     3901 acagtcaatt cgtcccatat tgcttccaga gtctggatag gatcgtcttt cgccccaatc
     3961 agtgtcgctg cattgatagc cccaatagaa acacctgaaa ttatgtttgg cgaaaaactg
     4021 tctgatgcat agaggtattt taatacgcct aactcataag cccccagtgc tcctcctccc
     4081 tggagtacaa tagctgtttg cttcatggat gtccttatca tttcaccctg tatgagctag
     4141 tatagcagtt aattataatg aaaaagatgt gatgcaagtc ctggattgtc agaagcaagg
     4201 tatattgcca tccagttaaa tcagcgctga ctttgatagg gtggggcgca aaaattggcc
     4261 gcaaacgatt ttaagcattt gttaagcatt ttgactttcc tggaaaaaat attattatga
     4321 ataattctat tagggattaa atggcctgtt tttttatagc ggcgatagct ccagacatac
     4381 aaagccttgc ttacgcgatg tgtattgttg taatgaaccg gagtaatcag ataaatgctc
     4441 tctaaagctt gatgatacct acatctgaat aagattattc ttataaaaca ggaagatttg
     4501 atggaagttg accatattaa aaatgaagta aaattatttt tactcaactc ttttcgaatt
     4561 aaggatattg gttattcgga taatattttt gatacaggcg caatgcactc tttatttttc
     4621 attcaattat tagtttttat agagaagaaa tataaaattg atcttgaggt cggtgagttt
     4681 gatctgaacc agttaactag tattgatgca atcgctcact ttatttccag taaactataa
     4741 ttgtaatagg tcactcccct atgttggatt tagggcaaac agccagagtt gagcttttac
     4801 ttataattca atacgaatag ctgtgataag ctttggtgga caaacggcat gaaacaaaca
     4861 aaacttactc ttctgggtgc ggggacaatg ggttcgggta tcactcagct ttttgcacaa
     4921 tacggatttt atgtcacttt aatcgataac ctccaatctc aattggataa agcaaaagac
     4981 actatcgcta aaaatttaca ctatctggca ttaactcaaa atttagaatc gacacattct
     5041 attgaaacca tcctggcatc aattaccttt acaacaaagt tagatgagct taagcaatcg
     5101 gaatatatta tagaaaatat cactgagaac tgggagcgga aaaaagcgtt gtatcaggta
     5161 ttaaaaaaag aatgtagcgc aacctgtatt ttgggggtaa acacttcatc catccccatt
     5221 actaaaatcg catcacttgt tgatcaccct caacgtgtta taggtgttca ctttatgaac
     5281 ccggcaccga tgatgcctat ggtagaggtc attaagggat atcacactga cgaattgaca
     5341 atagaaaaaa caagaacatt actagaacaa gtacataaaa aaatgatcgt tgttaaggac
     5401 tctgttggtt ttgtgagtaa ccgagccatg atgattttta ttaacgaagc catctttatg
     5461 gtacaggaaa acattgcatc aatcgaagac atcgatgttc tatttaaaca atgctttggg
     5521 cataagatgg ggccgctgca tacggctgat ttaattgggt tggatacggt tctctattct
     5581 ttggaaacca tctattcaga attaaacgat ccaaaatatc gaccatgctg gttattaaga
     5641 aatatggtgg atgcagggct tcttgggcaa aaaacaaaaa agggatttta tcaatatgaa
     5701 tagggggtag tttaccattc gctatttcaa cccggatggt taaattgtaa acaaacacta
     5761 gggagagcaa ttttgccaaa ttaccagcct caatacgtga agcaacagcg actatttaac
     5821 caatttatag aaaattgcct tcactcccat gcaaaaattt ttgataagga acaaaacatt
     5881 ccgcgcgatt ttttttatac attagcccaa aatggttttt tgggcgctag tattcccaaa
     5941 caatttggcg ggcagcaatg ggatgaattg accattggta tcatgcatga atcctttgct
     6001 aaagaacttt gttctttggc aaatatccta acagtattgg gcatggtgag taagtcactt
     6061 ttgcaatttg gttcaaaagc tcagaaacaa atctggctgc cacggattgc ctcaggagaa
     6121 actttagctg cattggcttt aactgaatct aatatcggta gtgatttgga gcatgttgaa
     6181 actcagttgc tggatgcagg tgatgaattc attttaaaag gaagtaaaaa atacattact
     6241 ttagggcaaa tagctgattt gtttttagtt ttagcaaatt gccaaggcca actgactgcg
     6301 gttttagttg aaaaggacac gtcaggctta atcattacac ccatgtctaa ttttttagga
     6361 ttacgttcta atatgttggc agaaattttt tttgataatt gccgaattcc taaaacaaat
     6421 ctgttgggta gtatcggtat cggctttaac tgtgttattc aaactgcttt agatgaagga
     6481 cgttatacaa cagcgtatgg atgtgttggt ttaggacaag cttgcctgga tgcggctctt
     6541 ggctatactc aaaaaagaaa acaattcgat cgcagattag acgaacatca gctcattcaa
     6601 aaaatgataa ctgaaatggt ggcgcaggtt aaagccgccc gggaattgtg tttttatgcc
     6661 ggagaactta gacagcaaaa aaatccagca tacatagctg aaacattaat tgcaaaatac
     6721 gcggcttcaa aaatggtcgt ttttgtatct aatcatgctt tacaaatatt tggcgcagct
     6781 ggctttgaag aaacttatcc tgttgagcga tattacagag atgccaaggt aatggagatc
     6841 attgaaggta cctcacaaat gcacgagata ctcatttcaa agctttgcaa caataaatag
     6901 aggatattat taaacaatgg gatcccctga tcagtacacg acaatttgca ccttatttga
     6961 ggagaaggta aagcgatacc cggaaaatac agcagtttat ctggaagatc gccaaatttc
     7021 gtacgatgac tttaataaac aagtcaacca atacgcgcgg tatctgcaaa gaaaagggct
     7081 accattgaat ggaattgtcg gtattcaact cacgcgctct tttgaaatgc tggtggcaat
     7141 ttttgcagta ttaaaggcag gtggtgccta tttgccgata gatcccatgg cccctgttct
     7201 gagaaatcaa gccatccttg aagacagcca aatcaaattt ctaattgtca gtgatcattc
     7261 attattgacg gcaaccccgg gtttgcaagt gttaagcatt catgccaatg tgagcgatga
     7321 agattgccat aatttaaacc tgcccaggaa aaaacaggat ttagcgtatg tgatgtatac
     7381 ttcaggcact actggcaagc cgaaaggagt cctgatttca catcaagctt tgcttaatcg
     7441 tattttatgg atgcaaaaca catttccctt gcaaagtcat gatgtagtat ttcataaaac
     7501 acacatttgt tttgacatct ctgtctggga gacctgttgg tggtctatcg cgggcgccgg
     7561 agtagtactt ttaccgccaa gaaaagaaca tgacatcaag ctgtttattc aaatgattga
     7621 gcaataccaa atcaatgtca ttcattttgt cccctccgta ttacgaatat ttttaagtta
     7681 catcgaacaa gatttttcga taaatcgctt aagctcttta aagtacgtat tttcaagtgg
     7741 agaggcgctg gatgccaaaa gtgttaatct attcaaccag ttatttcaag atcaaaaaac
     7801 aatattagtc aatttatacg ggcccacaga ggccacaata gatgtttcct gttttatttg
     7861 tgaaaagcac aaacgttttg acactgtacc cattggaaag cctatccaaa atactcaatt
     7921 ttttgtgtta gacgagcagt taaattcaac cggcttcgaa cctggagagc tttatatttc
     7981 cggagttgga ttagctcagg gttatttaaa taatccagtg ctgactcagg catcgtttat
     8041 agacaatcct tacttgcctg gagaaaaaat gtatcgtact ggagatatag tccgttggaa
     8101 tcaagagggt gagttattat ttttaggccg caaggacgat caggtcaaat tacgtggcat
     8161 acgtattgaa ttaggagaaa tacagcatca tttattagcg catcccaaca tccatgatgc
     8221 cgtcgtggtc tgtgagaaag tagattgtct tgatcataga atggttgtat tcctgattgc
     8281 taataacaaa caaacaaaga ttggcagcgc agaactgaaa tcgtttttaa aaaacaggct
     8341 cccggattat atgataccag agcattatct atggttaagt gcatttccaa taaaagaaaa
     8401 tggaaaaata gacaaagaga agctattgag tttcatttag catgagcaat gcccgagaaa
     8461 taaaaataat cagggaaaat gacttatact taattaaaag ccggttgcag aaataatata
     8521 aactatgttt tatgttatga acccgtcata cgtcgtttaa ttagtgttta ttaaattctt
     8581 ggtgcggcca agaaatggag ccataaatta tgagtgacaa taactactca aacaaaattg
     8641 ctattgttgg tatggctgta aaatttcctg gggctaatga tcttgatgaa tattgggatt
     8701 tattgaagtt tggtaaagaa gcagtcacta aattttccga agaacaattg catcggtcag
     8761 gtatttcaga gcaattaatc gctaatccct attacaaacc atacagaggg atattagatg
     8821 acctggagtc attcgatacc gctccgtttg aaaataccac caaaaatttt gaattgttgg
     8881 gtgtgcaagg caaagtgatg tcgcatttaa cttatcgggc tttgagcacc atgagaagtc
     8941 aggagcatca ttcaaataac tacataaaaa atacggctgt ttatattggc gccaataatc
     9001 aacctgcctc ccatttcctg ggagccgcct actaccagtc gattgatact caaaaagcga
     9061 tagaaaggta ttattcaaaa gtcattgcac cttatatttc ttatcaattt ggatttcagg
     9121 gtagttctat tgatttatat acagcttgct cgtcttctct gacagccgtg atccaaagct
     9181 gtagagagtt aaacagtcat caatgtgata tggcaattgc aggcgcgtgc ttaatcgatt
     9241 taccccagga agttggatat ctttatgaag agaatggctt gttttcctcc gacggacatt
     9301 gccattcttt tgatgtttca gcatgtggca ccttatattc aaacggtgct ggattggttg
     9361 ttctaaagcg gcttgaagat gccattgcgg ataatgatct aatccatgct gtgattatcg
     9421 gatccgcgat gaataacgat ggtaattctt caaaagaagg atttatggca ccagggatcc
     9481 atggccaata tacttgctta cagtcggctt ggaaaaacgc ggggattgaa ccaggcgacc
     9541 tggattatat tgcagctcat ggtgcttcaa ccaagctggg tgatgcaaca gaaatatatg
     9601 ccttaaaaaa agcgttcaaa gacgttaaaa agacacattc ttgtccaata agctcagtaa
     9661 ttagtaatct ggggcatact ggtattgtgt ccggtatggc gtcaatcatt aaaactgtgc
     9721 ttatgctcaa aaataaggct gtagtccctt caattaattt tgaaactcct aatccagatt
     9781 ttatgttgga agactctcct atatacattg ccactcaatt tcaggatttc cctcaaatta
     9841 aaaaacacta tttggccggt gtttccagtt atggcgcagg gggaagtaac acacaccttg
     9901 ttttgcgtag ctatgatgaa aactagggtt ctgttgatat ttcattttta ttcctgataa
     9961 aagttgacgt actgcggttt gttcgttaga gactcacata caatttaaac agcaaggagt
    10021 atgtcaattt gtttacgttc ggcctgagga gtggctaaag ccacgtctcg aaggccatcc
    10081 ttcacaggct tcgagatggt gcagatgggg attgggccaa actgatcttg gtatccaaat
    10141 tgtatgaggt ttcctcagcg gcaaaccaga gtaaatgaag aaatggcggt taaacgcgtt
    10201 ctcatcttgg tttgccatag agaaaataat ttctgttact atgattggtc agccgagttc
    10261 aatggtataa agcggtttag cttgcccgaa tacaaatatt taatttcagg tacctcagtg
    10321 atattgactt gggcatctac caaacccact ttgcttaatc gtaagcgtat attcttttgt
    10381 aattcgctct tgtttatata accactggtc acaaccttga tattaacccc atcaattgtt
    10441 tgttcgacct gatattcctg aatatttttt tctggcaaga gtgctcccaa gaaaagcgaa
    10501 tgatgtacaa aaatattacc aggataatta aaatcacatc caggccgacc tttaggggtt
    10561 tgtataagct gatggtggat gccacaatca caggttttat ttaaaaataa aatttcatct
    10621 gaattttcat agcggattaa tggtaaagtg taattatata aattagttat atacattttg
    10681 gagaccataa ttcctttgtc aacagggcga ttttgttcat ccagaggttc aataatgcat
    10741 tgatcatcat tcaagtgcat ctcatccaca ttcgcccggc aatttagccc aaccatccct
    10801 tcagtacaac caaatacatt aaaaatgtcc acttgaggcc aggtttcttt gattaaagcc
    10861 aaggtttgct caaaaagcgg ctctcctcca acaaaaagaa ttttgggttc aatttttatt
    10921 tgtccttttt gaaccaattg acatactttg tgaatatatg aagggccggc acttaagata
    10981 tcgggttgga tttgattgag acgagtgata actatgttta atggggttac agccataggc
    11041 agaaaataca tattttgatt acctcgccca aaggtttttg ctaaagaata ggctgcaatt
    11101 gctgtatttg ttacaaacag gctgacaatt cttgggtttg tgtttagctt ggtagttaca
    11161 atttgcgtgc gttcatagtt gtaaagtgga tagcggacaa atgcggtgtg aaatgtaatc
    11221 cattcatccc aatcataaat aaatacgcca cgtttcccac tgcttcctcc agttgacacg
    11281 acctgatagc gggagaataa atatagggta tcaatgctgt catttttttt actcagatgc
    11341 ttttcaacca gatttaatga aagtttggga tcagtgacaa ttgcatccca attttccatt
    11401 aaaatggttt tatttatagt agggagctcc tctaatcgct ctctggtaaa gttttctatg
    11461 ttgatttttg ccagtgtttt tttataccag ggagattttg ttttcgcgtg aagtaatatt
    11521 gtttgcagtc ttttattgat taggtcttgc agtatgtctt tagaataata gatagcttcc
    11581 tgattttttt gaaattgttt ctcgcagtct tgaatatgct gccatatcag ttgatctgaa
    11641 gttatggagt tataattctt cttatagatt tggttaacca agtgattggc tttgtttatt
    11701 aagaggtcaa aaaaagacat attactcccc tttttcccca gggcgctatc cttacactat
    11761 aagccatggt ctttgagata acccataatt agtggtgata ttcctaaacg ggattttaat
    11821 caataggtag cttggattaa tttttgattg gcagtcacca taagattaaa atacctaaga
    11881 agattatagc ttaaaaccaa gcaggatatt ctattataca aggtagtgcc gttatcagtc
    11941 attttcagtg cggtttaatg atttatgcaa ttgcaaagag ttctcatcga ggttagaagg
    12001 tgatgatgtt aaagtcgatg ctatccacag ataatttgac aggacttacg aaaaacccca
    12061 atctctatgt taaaaaatgt ttttaattcg gtcatttagt ttatctaaac tccctcatta
    12121 aaaacatttt ttgccttgac ct
//
LOCUS       DATNSN010000104        12135 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.104, whole genome shotgun sequence.
ACCESSION   DATNSN010000104 DATNSN010000000
VERSION     DATNSN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.104"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..423)
                     /locus_tag="VAH76_002178"
     CDS             complement(<1..423)
                     /locus_tag="VAH76_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEO1386784.1"
                     /translation="MMLDRTDKKILEILQANCEINNQELADLVALSPSSCLRRVKLLI
                     DNGYIKKQVALLEPEKIGLKLTVIVLVGLNNHQPEVMNQFEKTIHLFPEVIQCYLITG
                     QSADYLLKIIVPDLNAYQTFLLGKLTRINGVASVHSSFI"
     gene            624..1856
                     /locus_tag="VAH76_002179"
     CDS             624..1856
                     /locus_tag="VAH76_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386785.1"
                     /translation="MSVSRVTAWFVWIIVSIFYAYQYVLRVMPNIMLDDITQQFHIDA
                     TIFGQFSGIYYIGYSLMHLPIGIMLDRFGPRKVMTGCIMLTVIGLLPILFAENWVYPV
                     IGRALIGIGSSAAILGTFKIIRMTFKEQHFPRMLSFSVMIGLIGAVYGGGPVSYMCQL
                     MGYKMVVEIFVGIGIMLAGITYLIVPDVQPSRQDSVISNIRMVFGNSKVIMLCCFAGL
                     MLGPLEGFSDVWGSGFLKMAYSLNTNTANYLPSMIYIGMCFGAPALSLIAEKTGYYLG
                     TIIASGVVMFLVFTMLVGGVLTVPGITISFILVGICCSYQILAIYKVSTYVPDNLAGL
                     TTAIANMIIMIFGYGFHSVIGYVVNVYGGTHAARAFVYGVGVIPVTLAVGVMGFLFLA
                     YQEKNFSRDASFVSPELR"
     gene            1911..2225
                     /locus_tag="VAH76_002180"
     CDS             1911..2225
                     /locus_tag="VAH76_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HEO1386786.1"
                     /translation="MYMLYFHVPETHLDEVKNAVFAAGAGHVDNYTHCAWQTLGEGQF
                     MPLTGSQAFIGEINQLEKVPEYKVETICAGERIKEVVAALKKAHPYESPSYQVVRIET
                     EF"
     gene            2416..3969
                     /locus_tag="VAH76_002181"
     CDS             2416..3969
                     /locus_tag="VAH76_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEO1386787.1"
                     /translation="MLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSA
                     FQNANWWKVFNDPTLTVLIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMV
                     GDYNYNRIGGNQLEGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAY
                     DSALVTLTSDIASTFIKIRTSEELIKVTKANIQVQAMSLKIAKDRYSGGQTSLLDVEQ
                     AATELAETQAKLPQYVSDLQHQKDILAVLLGTTPDKVDDLLKKNKGIPKASAVVEVGI
                     PKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGTFVFASNNIGSSTISDIF
                     DWSNRNIVAGPSLTWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITR
                     YVEGQKTVSSLKTANASAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATG
                     ELALSLVGLYRSLGGGWQIRGKNDIVSNQIKSEMAARTNWGSLLKQENHLPPDSNGQR
                     MKQLYLPTW"
     gene            3975..5129
                     /locus_tag="VAH76_002182"
     CDS             3975..5129
                     /locus_tag="VAH76_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1386788.1"
                     /translation="MNSQNKQNLVKTVAIVFFGIVFVYLVYDHFTRTQVATPAPPFVI
                     VQKPKLVKMGQYVTQTGNTVAFQSVDLVARIEGYLDAIKFTDGAFVKKNQELFIIEPE
                     PYLEQLRAAEAKVAAQKANYAYAKSEYARQQRMYKQNATSLNSVEKWKAKVEEAQAEV
                     DKSEADAKNAAITYGYTHLSAPFDGRMGRHLVDVGNLVGHGAATDLATIEQIDPIYVY
                     FNLNELDLIKIRNAARKRGYKPMELVKIPVEVALQDEKGFPHKGTLDFVNTGLNASTG
                     TMEFRALLPNKGYPLVPGLFVQVRVPISEPSPKLTIPDTSVQYDQIGPYVLVVDKDNY
                     VLTRRVTLGGLENGVRAITKGLDAQDRVIVSGLQNATPNHQVTPKTEQPE"
     gene            5180..8332
                     /locus_tag="VAH76_002183"
     CDS             5180..8332
                     /locus_tag="VAH76_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEO1386789.1"
                     /translation="MISKFFIERPVLANVIALFIVFIGIIAIAVLPVSQYPAIVPPTI
                     QVTTSYPGADAKTLINTVALPIEQQVNGVEDMLYMQSTSTSGGTYTLIVTFAIGTDLN
                     YAQVLVQNRVQAAMAQLPESVQKQGVVVQQKSTAILQFITLTSKNDEYDGVFLDSYAT
                     INMQDELSRLPGVGNVVVFGSGSYAMRVWLDPKKMLAFSLNPSDVLNAISYQNKEVSA
                     GQLGAPPTVGKQAYQFTVNVPGQLSDPKEFENIIIKTIDTSPDENSSASSSAQVVRIR
                     DVGRVELGSESYNQLANLNGKPTAAIGIFQLPGANALDVAQEVRKAVAKMAKQFPPGL
                     EYSIPFDTTVFVKASIEEVYKTLFEAGILVLIVIVVFLQNFRASLVPATTVPVTIIGT
                     FFAMLLLGYSINLLTLFALVLAIGIVVDDAIVIVEGVTQHIEKGMSPKESAILAMKEL
                     FGPIIGITLVLMAVFVPAGFMPGLTGSMYAQFALVIAATAFISAINAMTLKPTQCALW
                     LKAPDTSKPKNIFFQAFDRIYNPIESAYVKFIDRLVHRSGKVCLIGIILVACAIYGLT
                     RIPTGFIPMEDQGYLMLSVQLPDGASLGRTDEVVSRLAKKASEVGGVDNVISIDGISL
                     LDNNSLLPNAGVIYVIFKDWSVRGKSENLRALYTKFNAMAKETLDAKVLVVVPPPIQG
                     LGMSGGFQMQVELQDGTFDYRKLQQATDQMINTGHQYPQLQNLMTTFRASVPQVAAPI
                     NRTKAESLGVRVADAFDTLQTYLGSSYVNLFTKFGQVFPVYVQADASSRVSSEDLRNY
                     YVRNQSGSMVPLGTLTDVGPAVGPSIISLYNLYPSSNINGVAARGYSSGQGIQVMEEL
                     AKEQLPPGISYEWTSTAYQEKVAGNLSYFIFALSLVLVYLILSGQYENWLIPSAIILS
                     VPLTLVGTVLALGSLGMDNNMYTQIGLLLLIALATKNAILIVEVAREQREIHNKSVLE
                     AAVIGAKTRFRPILMTSFAFIMGVMPLVFATGAGANSRRSIGIAVSSGMLASTCLAVV
                     FVPVFYVLLQTWQDKRKAKH"
     gene            complement(8639..11380)
                     /locus_tag="VAH76_002184"
     CDS             complement(8639..11380)
                     /locus_tag="VAH76_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SdcA"
                     /protein_id="HEO1386790.1"
                     /translation="MVVNMVDVIKFKEPERCDYLYIDENNKVHILLPIVGGDEIGLDN
                     TCQTAVELITFFYGSAHSGVTKYSAEHQLSEYKRQLEEDIKAINSQKKISPHAYDDLL
                     KEKKERLQQIEKYIELIQVLKGQNAEQDIRQLRTGGIPKLPAAVKEIIKSSENAFAVR
                     LSPYDNDKFTRFDDPLFNVKRNISKYDTPSRQAPIPIYEGLGYRLRSTLFPKEKTPTP
                     INKKSLREKVKSTVLSHYKEEDRIDGEKRDEKLSELITKLQNELVKELVKIDPQYSKL
                     SLSKDPRGNEINYNYLVNSLMLVDDDSKIGEWIETILDSTVDSTVWEAQASSPFYDGA
                     KEIKSDKEADRISIRVQYLLAEANIYCKTNKLSDANFGEFFDKEPHATEIAKRVKEGF
                     TQGAEIEPIIYDYINNNHAELGLKSPLTGEQLQEITEKFTRHYNTIKESPHFDEFFVA
                     DPDKKGNIFCHQGRISCHFLDFFTRHTKGNYPLGDLANHQESLQKGTSNRLHHKNELV
                     AQGYERLDQFKKEVVKLLAEDKPKELLDYLLTNSPTGVPNYSMLSKETQNYIAYNRNW
                     PAIQKELEQTTNIPENQKQDLLRLLSRDNLSHDNLSAITWSKYSSKPLLDVELNKIAE
                     GLELTAKIYNEKRGSEWWFKGSRNQARNTQCEELQRVAKEINALLQNETLTKSQVLEK
                     VLNSIETLDKIDRDISAESNWFKSTLQKEVRLFRDQLKDICQLDKYAFKSTKLDEIIS
                     LEMEEQFQKIEDPVVQKIVRDLPAHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREIN
                     KSTDKKTLLEQDIPKLFKEVNALLLSKLKEEKAIDEQVHEKLSQLADKIPPEHFTRNN
                     IIKWSATPEKLEESNLNELIKSVESPTTKQTSKQFREAMGEITGRNEPPKDTLGHTI"
     gene            complement(11531..>12135)
                     /locus_tag="VAH76_002185"
     CDS             complement(11531..>12135)
                     /locus_tag="VAH76_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein SdcA"
                     /protein_id="HEO1386791.1"
                     /translation="QLFRDQLKDICQLDNYAFKSIKLDEIISLEMEEQFQMIKDPAIQ
                     QIVRDLPSHCHNNEAIEFFMTLNPEEAAKVASYLSLEYRELNKSTDKKTLLEQDIPKL
                     FKEVNMQLLSQLKQDSAVKEDVFEKLSQLADKIPPEHFTRNNIRKWSANPEKLEESNL
                     GELLKSSDSSLTEMARKYRDTINEMTRRNEPPRETVRHTI"
BASE COUNT         3388 a         2195 c         2533 g         4019 t
ORIGIN      
        1 tataaagctg gaatgaacgc ttgcaacgcc attgatgcgg gttaattttc ctaataaaaa
       61 agtttgataa gcgtttaaat cgggcacaat tatcttcaat aaataatcag cagattggcc
      121 tgtaatcagg taacattgaa tgacttctgg gaaaaggtgt atggtttttt caaattgatt
      181 cataacctca ggctggtgat tgtttaaccc tactaaaaca ataacggtca attttaaccc
      241 tattttttct ggctcaagca gggcaacctg tttcttgata taaccattat ctattaataa
      301 tttgactcgg cgcaaacagg aggaaggaga caaggccaca aggtcggcca attcctggtt
      361 attgatttcg caatttgctt gcagtatttc aagtattttt ttatcggtac gatcgagcat
      421 catatcttat gcaatgaaaa tggcattgat atttcttaat atagaattat attccaaaaa
      481 aatctattaa attagaattt ttaatcaaaa tagatggttt gattttgttt tttgcaatcc
      541 aatttcaagc gaagtaagat aaaatacttt atgacaatta tttgatcttc atgatctttt
      601 tatctgcttt tctgagggtg attatgtctg tttcacgagt tactgcgtgg tttgtgtgga
      661 taatagtatc catattttac gcatatcaat atgttctgag agtaatgccc aacattatgc
      721 tggatgatat tactcagcaa tttcacattg acgctacaat ttttgggcaa ttttccggta
      781 tttactacat tggttattct ttgatgcatt tgcctattgg gattatgctc gatcgttttg
      841 gcccaagaaa agtgatgacg ggatgtatca tgttaactgt gattggttta cttcctattt
      901 tgtttgccga aaattgggtg tatccagtga taggaagagc tctgattggc ataggctcct
      961 cggctgccat tttaggaaca tttaaaataa tccgcatgac atttaaggag caacattttc
     1021 caaggatgct gagtttttca gtcatgatag gacttattgg cgcggtatat ggcggaggtc
     1081 ctgtcagtta catgtgtcaa cttatgggct ataaaatggt ggtggaaata tttgtaggca
     1141 taggaataat gctggccggt attacctatc ttatcgtccc cgatgtacaa ccctctcgcc
     1201 aggatagcgt gatttctaat ataagaatgg tttttggcaa tagtaaagtg atcatgttgt
     1261 gttgttttgc cggattgatg ttgggaccgc ttgaaggatt ttctgatgta tggggttctg
     1321 gctttttaaa aatggcatat agtttgaata caaacacagc aaattatctt ccttcaatga
     1381 tctatatcgg tatgtgtttt ggtgctcctg ctttaagtct gatagcagaa aaaacggggt
     1441 attatcttgg aactatcatc gcatcaggcg tggttatgtt cttggtattt accatgcttg
     1501 tcgggggcgt actcacggtt cccggtataa caatcagttt tatcctggtt ggtatttgtt
     1561 gctcttatca aattttggcg atttataaag tatccactta tgtccccgat aatcttgcag
     1621 ggcttactac tgcaattgcc aatatgatta ttatgatttt tgggtatggt tttcattccg
     1681 tgattggata tgtcgtcaat gtgtatgggg ggacacatgc agctcgagct tttgtttatg
     1741 gagttggggt gattcctgtg acattggctg ttggagtgat gggctttttg tttctggcct
     1801 atcaagagaa aaattttagc cgggatgctt cctttgtttc tcctgaatta cgataattac
     1861 tggcattttt atcttgtggg gtaataaata tcaagggagg cattgctatc atgtatatgt
     1921 tgtattttca tgttccagaa actcatcttg acgaagtcaa aaatgcggtt tttgcggcag
     1981 gggcagggca tgttgataat tatactcatt gtgcctggca aactttaggt gaaggtcagt
     2041 ttatgccttt aacaggtagt caggcattta ttggagaaat taaccaattg gagaaagtgc
     2101 ctgaatacaa ggtagaaacg atttgcgccg gagagagaat caaagaggtg gttgcagctt
     2161 taaaaaaagc tcatccctat gaaagccctt cttatcaagt ggtgcgtatt gagacggagt
     2221 tctaaatatc agacaggata attcaggcag gttgaatcac cagatggttg gtcaattagt
     2281 aagaatttgt ttcttcagga tacttgttca attgagtatt catagataat atctaatgat
     2341 tgatgaacca atagttcaaa tccatcttca atctacataa gctgcttttg aacagatagg
     2401 gaaatactgg tatacatgtt gaaaatcctt gctctcttaa gttgtttgat tctaagctcc
     2461 tgtctggtcg ggccaaattt taaagagcct aaaaaagatg ccgccaatta ttggcttact
     2521 tcaagccctg caataaaaca atccgctttt caaaatgcga attggtggaa agtctttaat
     2581 gatcccactt taacagtctt aatcgataac ggttatcata ataatctttc cttgcaaatg
     2641 gcaggagtac gagttttgca agcaagagct caacttgctc aatcggttgg ggagctttat
     2701 ccccagcaac aagctatggt gggagattat aattacaacc gaattggggg taatcaatta
     2761 gaagggcttc tgcctcccga ttttttaacg gccagccttg gattttccgc aagctgggaa
     2821 cttgattttt ggggaaaata ccgaagggcg attcaatcta atgatgcctt gttccttgcc
     2881 tctcttgccg cttatgattc tgcattggtt actctcacgt cagatattgc cagtactttt
     2941 attaaaatcc gtaccagcga agaattaatc aaagtaacaa aagccaatat ccaggtgcag
     3001 gcgatgagtc ttaaaatcgc aaaggatcgt tattctggtg gtcaaaccag tttactggat
     3061 gtggaacaag cggcaacaga acttgcggaa acccaagcaa aactgcctca atacgtgagt
     3121 gatctccaac atcaaaaaga tattttggct gtattacttg gtactactcc cgataaagtg
     3181 gatgacttgc ttaaaaaaaa taaaggaata ccaaaagctt cagcggtagt ggaagttgga
     3241 attcctaaag aagtgatagc acgaaggcct gatgtctacc aagccagaat ggaggctata
     3301 gcccaatcag ctgcaattgg cgctgtgaag gctaatctat atccttcact ttcattggtg
     3361 ggtacttttg tctttgcttc aaataatatt ggcagttcta ctataagcga tatatttgac
     3421 tggtcgaatc gcaatatagt cgcaggtccg tcactgacat ggtctttatt aaattatggt
     3481 caaatcacga atgcagtacg ggctcaagat gctgctttcc aacaagcctt gctcaattat
     3541 cttaatttgg tgctcaaagt gcagcaggaa gttcaagaca atattacgcg ctatgttgag
     3601 gggcaaaaaa cagtctcttc acttaaaaca gccaatgcct ctgctataga atcaacaagg
     3661 cttgctttag tgcgatataa ggaaggagaa gcaaattata ccactgtgct gaatgcagaa
     3721 cagcaacaat tgcgagtaca aacctcgctg gtcaatgcca caggagaatt ggctttgtct
     3781 ctggttggct tataccgctc acttggaggc ggttggcaaa tacgaggcaa gaatgatatt
     3841 gtttcaaatc aaataaaaag tgaaatggct gcccgtacta attggggtag tttattgaag
     3901 caggaaaatc atttgcctcc ggattctaat ggacaacgaa tgaaacaatt atatttacca
     3961 acctggtgat gagaatgaac tctcaaaata aacaaaattt agttaaaacc gtggcaattg
     4021 tattttttgg tattgtcttt gtttatttag tatatgacca ttttacaaga acccaggtag
     4081 caaccccagc accaccattt gtgatagttc aaaagcccaa gttagtgaaa atggggcagt
     4141 acgttacaca gacaggcaat acagtagcat ttcaatcggt agatttggtg gcacgtattg
     4201 aaggctattt agacgccata aaatttactg acggtgcttt tgtcaaaaag aatcaggaat
     4261 tatttattat tgaacccgag ccttatcttg agcaattgcg tgcggccgaa gccaaggtag
     4321 cggctcaaaa agcgaattat gcttacgcca aatcagaata tgccagacag caacgcatgt
     4381 ataaacaaaa tgccacgtct cttaacagtg ttgaaaaatg gaaagcaaaa gttgaagaag
     4441 cacaggcgga agtggataaa tcggaagctg atgccaagaa cgccgcaatt acatatggct
     4501 atacccacct ttcagctcct ttcgatggac ggatgggaag acatttagtc gatgtgggga
     4561 atttggttgg gcatggtgct gcgacggatt tggctaccat agaacaaata gaccctattt
     4621 acgtttattt taatttaaat gaactggatt taatcaaaat tagaaatgca gctcgcaaga
     4681 gaggatataa gccaatggag ctggtcaaaa tccctgttga agtagctttg caagatgaaa
     4741 aaggatttcc tcataaagga acattggatt ttgtaaatac cggtttaaac gcttcaactg
     4801 gaactatgga gtttcgtgcc ttgttaccta ataagggata tcccctagtg cctggtttat
     4861 ttgtgcaagt ccgggtaccg atatctgaac cctctccaaa attaacaata cctgacacct
     4921 ctgtgcaata tgatcaaatt gggccctatg tgcttgtggt tgataaagat aattatgtgc
     4981 ttaccaggcg tgtgacactt ggcgggctag aaaatggggt aagggctatt accaaaggcc
     5041 tcgatgctca ggacagagtg attgtttccg ggttgcaaaa tgcaactccc aatcatcagg
     5101 ttacgcctaa aacagagcaa cctgagtaaa aaaactgtaa gaatttaaga tacgctattc
     5161 agggagcgaa acaactgaaa tgatttccaa attttttatt gaaagacccg ttctggccaa
     5221 tgtcatcgcc ttgtttattg tatttattgg aataatagcg attgctgttt tgcctgtgtc
     5281 tcaatacccg gccattgttc ctcctactat ccaggtgacc accagctatc ctggtgcaga
     5341 tgcaaaaacg ttgattaata cggtggctct gccaattgag caacaagtca atggggttga
     5401 ggatatgctg tatatgcaat cgacaagcac cagcggaggc acttatactt taattgtcac
     5461 atttgcgata ggcactgatc tgaactatgc gcaggttctg gtgcaaaatc gagtacaggc
     5521 agctatggct caattgcctg agtctgtgca aaagcaaggt gtggtcgtgc agcaaaaatc
     5581 gacagccatt cttcaattta ttacgttgac gtcaaagaac gatgaatacg atggcgtatt
     5641 tcttgacagc tatgcgacca tcaatatgca agatgaatta tcccgtttac ccggggttgg
     5701 caatgtggtg gtttttggtt caggaagtta tgcgatgcgg gtttggctgg atcctaaaaa
     5761 gatgcttgct ttttctttaa acccaagcga tgttttaaat gccatcagct accaaaacaa
     5821 agaggtgtca gctggacaat tgggtgctcc tcctactgtt gggaaacaag cctatcaatt
     5881 tacagtgaat gtgcctggac aactctctga tcccaaagag tttgaaaaca ttataattaa
     5941 aacgattgat acatcacccg atgaaaactc ttctgccagt agttctgctc aggtagtacg
     6001 tattcgtgat gtagggcgag ttgagttggg atctgaaagc tataatcagc tcgctaatct
     6061 taatggtaaa cctacagcag caataggtat ctttcaactt cctggtgcca atgcgttgga
     6121 tgttgcgcaa gaagttcgta aagctgtggc gaaaatggcg aaacagtttc ctccgggtct
     6181 tgaatattct attccatttg ataccaccgt tttcgtcaaa gcttcaattg aagaagtata
     6241 caaaacgctc tttgaagcag gaattttggt tttaatagtg attgttgtat ttttacaaaa
     6301 ttttcgcgct tcattagtac ccgcgacaac ggttcctgtg actatcattg ggacattttt
     6361 tgccatgctt ttattgggct atagtattaa tttgctgact ttatttgctt tggtgttagc
     6421 aattggaatt gtggttgatg atgccatcgt tattgttgag ggtgtgactc aacatattga
     6481 aaaaggcatg tcacctaaag aatcagccat acttgcaatg aaagaattat ttggcccgat
     6541 tattggtatc actttggtat taatggctgt ttttgtaccg gccggtttta tgcctggttt
     6601 aacagggtca atgtatgcac agtttgcttt ggttattgct gcaactgcct ttataagcgc
     6661 aattaatgcc atgacgttaa agccgactca atgtgcttta tggttaaagg ctcccgatac
     6721 gagtaaacca aagaatatat tctttcaggc ctttgatcga atttataatc ccattgaatc
     6781 agcttacgta aaatttattg atcggcttgt acatcgaagc ggtaaagttt gtttgattgg
     6841 aatcatttta gtggcttgtg caatttatgg cctgacgcgt attccaactg gctttattcc
     6901 aatggaagat caaggctatt tgatgttaag tgttcagctg ccggatggcg ccagtttagg
     6961 gcgtaccgat gaagtagtga gtagattagc taaaaaagca tctgaagttg gcggggtaga
     7021 taatgtgatt tctattgatg gcatttcatt attggataac aattctctgc tccctaatgc
     7081 cggtgtcatc tacgttatat tcaaagattg gagtgtcagg ggaaaatctg aaaatttacg
     7141 agctctttat accaaattca atgcaatggc caaagaaacc cttgatgcaa aagtattggt
     7201 ggttgtgcct cctcccatcc aaggtttggg gatgtcaggg ggatttcaaa tgcaagttga
     7261 attgcaagat ggtacttttg actacaggaa attacaacaa gctactgatc aaatgattaa
     7321 tacagggcat caatacccac agttgcaaaa ccttatgacg acatttcgtg cgagtgtacc
     7381 ccaagttgcg gcacctatta acagaaccaa agccgaatct ttaggagtcc gcgttgctga
     7441 tgcctttgat actttacaaa cttatttagg ctcttcttat gtcaatctgt tcacgaaatt
     7501 tggtcaagta tttccagtct atgttcaggc ggatgcatcc tcgagggtta gcagtgaaga
     7561 tttaaggaat tactatgtaa gaaatcaatc cggctccatg gtgcccttgg gtactcttac
     7621 agacgtgggg cctgcggtag gaccctcaat tatttcttta tataaccttt atccatcaag
     7681 taacataaat ggagttgctg caagaggtta tagctcgggg cagggaatcc aggttatgga
     7741 ggaattggca aaggaacaat tacccccagg gatatcttat gaatggacaa gtacagctta
     7801 tcaggaaaag gtggctggta acctcagtta ctttattttt gccttgtctt tggttttggt
     7861 ttatttaatc ttatctggcc agtatgaaaa ttggcttatt ccatctgcga ttattctgag
     7921 tgttcctttg actttggttg gaacagtatt agctctcggc agtttgggta tggataataa
     7981 tatgtacact caaattggct tgttacttct cattgcattg gctacaaaaa atgctatttt
     8041 gattgttgag gtagcgcgtg aacaacgtga aattcataat aaatcagttc ttgaggcagc
     8101 ggtcattggc gccaaaacac gttttcgtcc tattctgatg acatcttttg catttatcat
     8161 gggggttatg cccttggttt ttgcaacggg ggcaggtgcc aattctcgtc gctcaattgg
     8221 tatagcggtg agcagtggta tgcttgcctc aacttgcctt gcagttgttt ttgtgcctgt
     8281 tttttatgta ttgttgcaaa cttggcagga caagaggaag gcaaaacatt aattaattta
     8341 acgtaagtta tggataaaga ttatataaaa accagtctct atccagagtg cgttcgggct
     8401 gaggagatgc gtaagcatcg tctcgaagcc ttggcatgaa acattaaatc ggatacgaga
     8461 tttcgagatg gcataaatac ctccgcaacc ggagtggttt cgagataaca actgatttta
     8521 tccataactc acgctaattt acagcgatag aagaacgctg tgagaataat tctaatcctc
     8581 tcacagccaa aaagtgtatc tatcttccgg aatattcaaa caagttcata ctatcaagct
     8641 atattgtatg cccaagggtg tctttaggag gctcatttct tccagtgatt tcacccatgg
     8701 cttccctgaa ttgttttgat gtttgtttag tagtagggct ttcgactgat ttgattagct
     8761 cattaagatt tgattcctca agcttttcag gggtagcaga ccattttata atgttatttc
     8821 ttgtaaaatg ctcaggagga attttgtcag ccagttgact gagtttttca tggacttgct
     8881 catcaatggc tttttcttct tttaatttgg agagtagtag tgcatttact tctttaaaca
     8941 gtttgggaat atcttgctca aggagagttt tcttatctgt tgatttatta atttcccggt
     9001 attccaggct taaatagcta gccactttag ctgcctcttc agggttcaat gtcttaaaga
     9061 attcaattgc ttcatcattg tggcaatgag caggcaagtc cctgacaatt ttctgaacaa
     9121 caggatcttc tatcttttga aattgttctt ccatttccag agagataatt tcatcaagtt
     9181 ttgttgattt aaaggcatac ttatccaatt ggcaaatatc tttcaattga tcccgaaata
     9241 atctgacttc cttttgcaaa gtacttttaa accaattgga ttcggcggaa atgtctctgt
     9301 caattttatc caatgtttct atagagttta aaactttttc aaggacctgg cttttggtta
     9361 aagtttcatt ttgcaaaaga gcattgattt ctttagccac tctttgcaat tcttcacatt
     9421 gagtattacg agcttgattt cttgaacctt taaaccacca ttcacttcct cttttttcat
     9481 tgtaaatttt tgcagtgagt tctaatcctt cagcgatttt gtttaattcc acatccaata
     9541 atggcttgga ggaatatttt gaccaggtaa ttgcgcttag attatcgtgg ctcagattat
     9601 cacgagaaag caatcttagg agatcttgtt tttgattctc tggtatgttg gtagtctgtt
     9661 ccagctcttt ttgaatggct ggccagttac gattgtaagc aatgtaattt tgagtttcct
     9721 ttgaaagcat ggagtaattt ggaacacctg tgggtgagtt agtgagcaag taatccaata
     9781 attcttttgg tttatcctca gccagtagtt ttacaacttc tttcttgaat tgatccagtc
     9841 tctcatatcc ctgagctact aactcatttt tgtgatgtaa ccgattggag gttccttttt
     9901 ggagagattc ctgatgattt gcaagatcac caagtggata attgcctttg gtatgtcgag
     9961 taaagaaatc cagaaaatga caactgattc tgccttgatg acaaaagata ttgccttttt
    10021 tatcaggatc tgcgacaaaa aactcatcaa aatgaggaga ttccttaatc gtattataat
    10081 gtctcgtaaa tttctcagtg atttcttgta gttgttcgcc tgttaacggg gattttaatc
    10141 ccaattcagc atggttgttg ttaatgtagt cgtatataat tggttctatc tctgcacctt
    10201 gcgtaaatcc ttcctttact cttttcgcaa tttcagtagc atgaggctct ttgtcgaaaa
    10261 attctccgaa gttagcgtcc gataatttgt ttgttttaca ataaatgttt gcttccgcca
    10321 acaggtactg aactctgatg gatatcctgt ctgcttcttt gtctgatttt atttctttag
    10381 cgccatcata gaaagggctg gatgcttgag cttcccatac agtcgaatct actgtagagt
    10441 cgagaatcgt ttcaatccac tcaccaattt tagagtcgtc atctacaagc attaaactat
    10501 taactaaata attgtaattt atttcatttc ctcttggatc tttagataat gatagcttcg
    10561 aatattgagg atcaattttt actaactctt ttacaagttc attttgaagt ttagtaatta
    10621 actcgcttaa tttttcatct cttttttctc catcaattct atcttcctct ttataatgac
    10681 taagaacagt gctttttact ttttctctaa gtgatttttt attgattgga gtcggtgttt
    10741 tctctttcgg gaacagagtt gaacgcaggc gataccctaa tccctcgtat attggaatag
    10801 gagcttgcct tgagggcgta tcgtatttag agatgtttct tttgacattg aataaagggt
    10861 catcaaagcg agtaaattta tcgttgtcat aaggagaaag cctcacagca aaagcatttt
    10921 cagaggattt aatgatttcc tttacagcag cgggcaattt tgggattcct ccggtacgga
    10981 gttgcctgat atcttgttca gcattttgac cttttaatac ttgaattaat tcaatgtatt
    11041 tttcaatttg ttgcaggcgt tcttttttct cttttaataa atcgtcataa gcatggggtg
    11101 aaattttctt ttgactattg atggctttga tgtcttcttc caattgcctt ttgtattcac
    11161 tgagttggtg ttcggcagaa tatttagtca caccactgtg cgcactacca tagaaaaatg
    11221 tgatcaactc aactgctgtt tgacaggtgt tatccagacc tatttcatct cctcctacaa
    11281 tcggtaaaag gatatgaact ttgttgtttt catcaatata tagatagtca caacgttccg
    11341 gctctttgaa tttgattacg tcaaccatgt ttaccaccat tttctagtgt cttgtttata
    11401 agtatattat aaagacaatt tgtttataaa acaacaaaaa gagttgcaat tacacttttt
    11461 gcttgattaa acataccacg caatcaaacc gtatttttta ttctcaattg cttaattcca
    11521 tactatgaag ctatatcgtg tgtctaacgg tttccctggg tggctcattt cttcttgtca
    11581 tttcatttat ggtgtctcta tattttcttg ccatttcagt aagtgagcta tcagatgatt
    11641 ttaataattc gccgagattg gactcctcaa gcttttcagg attggcagac catttcctga
    11701 tattattcct tgtaaaatgt tcgggaggta ttttgtcagc tagttgactg agtttttcaa
    11761 agacgtcttc cttaacggca ctatcttgtt ttaactggga taacagttgc atattcactt
    11821 ctttaaataa tttaggaata tcttgttcaa ggagagtttt cttatctgtt gatttattga
    11881 gttcccggta ctcgaggctt aaatagctag ctaccttagc tgcttcttca ggattcaatg
    11941 tcataaagaa ttcaatcgct tcattattat gacaatgaga aggcaagtct cggacaattt
    12001 gttggatagc aggatcttta atcatttgaa attgctcttc catttccaga gagataattt
    12061 catcaagttt tattgattta aaggcatagt tatctaattg acagatatcc ttcaattgat
    12121 ctcgaaataa ttgca
//
LOCUS       DATNSN010000105        12058 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.105, whole genome shotgun sequence.
ACCESSION   DATNSN010000105 DATNSN010000000
VERSION     DATNSN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12058
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.105"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1091
                     /locus_tag="VAH76_002186"
     CDS             <1..1091
                     /locus_tag="VAH76_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ras GEF/LegG2"
                     /protein_id="HEO1386792.1"
                     /translation="YIQLMNFCYLKGDLFGAGAIYTGLIANNLPNSIDRKQLSSESRL
                     IFKDCEEKFKRLFNLSTTYNEHTNLKRQFKTALIPALPSLLTVQIYTKDAYDNSVSDF
                     ERVQEKLRQLDIEHNRFLNQLQNDHSTWSKAYYDYMSNVYLPQSIFEYTTLLREYEEL
                     ITTHGGPIAKQFHEDKNSEHLVSFVESTLLENQSDLKLSPFQNERIVEMLSEIDVVKS
                     ECSSPDVFDDISWKLREMNCTLVKAKSIGDYTSKIFANLDTPYMEENTDEDPKPVLVE
                     ELQTDDVYQLHHSGMKLIRELEQAIEDHKPKVIYHCSGEANYGFFKEARRRGRHLSPE
                     PMDIEVMDVDVMDYDENDLRNQTVMITV"
     gene            complement(1157..3382)
                     /locus_tag="VAH76_002187"
     CDS             complement(1157..3382)
                     /locus_tag="VAH76_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1386793.1"
                     /translation="MSTESFRILVIDDNPAIHQDFMKILNVSKNSALLNKFDAELFDD
                     ETEQLGISLPEFEIDVATQGIEGIEKVKRSLEEGRPYPLAFVDIRMPPGIDGVETIKR
                     IWAIDPQIQIVICSAYSDYSWESTVNTLGLSDNLLVLKKPFDVIAVRQLASALTQKWV
                     LARETQKHTEFLNQLVEERTESLQQSFSLLRATIESSSDGILVVDLQGKIVDYNSQFV
                     KLWNIPESIIKKETSEPQLIQYMSTQLLNSEQHAQHVKEFSIHIDDSSMQIYHFRNGK
                     VVECCSKPHRVGLQTVGRVWSFRDITEQTRLKEKLEYQATHDALTNLPNRLLLIDRIE
                     HAISGYARHKMKFAILFFDLDRFKMINDSLSHEAGDQLLCAVAERLRSLVRKEDTLAR
                     LGGDEFVMLFQSFNSEEQIAGVAQKILKSFQKPFHIAERDINIHVSIGISVYPTDGKT
                     VNTLLSNADMAMYQAKFRGGNQFSFYTEKLNKKTNQQFQLELDLQKAIVHHEFFLLYQ
                     PQFTVNTHEIRSMEALIRWNHPQKGIILPLDFIPTAESSGLIVPIGEWVLRETCKQIG
                     AWRTMGLPEIRVACNVASKQLRQRDFPAMVEDLLKEYSVEPHCLELEITENVIIDKEI
                     QRTIKKLKSLGVLIILDDFGTGNSSLNLLKQVAVDSLKIDQSFIQNISKSPGDEAIID
                     AIIAIAQSMNFNIIAEGVETQNQLKFLKKRRCNDIQGFLMSKPIPPEEIEKLLQGNRT
                     K"
     gene            complement(3385..4800)
                     /locus_tag="VAH76_002188"
     CDS             complement(3385..4800)
                     /locus_tag="VAH76_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO1386794.1"
                     /translation="MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYEND
                     QDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLP
                     PSNVVLNLSSFLELVHPNDIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIAR
                     ANPHKNEISGVLMDISKRKIIEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNS
                     VNVSAIILKKNISKIHYKKLSAIKTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTL
                     HEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIHDVILNAVKSVILDK
                     HQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANASRTIVISIGRTEK
                     NHIMIKINDNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNG
                     NGEGAQFVLDLPINPKTLSPR"
     gene            complement(4825..5988)
                     /locus_tag="VAH76_002189"
     CDS             complement(4825..5988)
                     /locus_tag="VAH76_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEO1386795.1"
                     /translation="MKIESFQYIQNQGWSIPTFPALDSENTLILVFASPIFRANSKPI
                     EELAQAYKKSKIIGCSTAGEIFGPNIFDNSISVAVVQFESTKLQLAKTEVKSTEESYE
                     AGIHLSKSLHQKQNPQSIFVLSDGLNVNGSELIKGLNQHAGKGVIITGGLAGDGADFK
                     NTWIIYDGKIVDHCIGAVGFYGNQIHVGHGSRGGWDIFGPERIITRSKANILYELDNQ
                     PALKLYKTYLGDRASELPAAGLLYPLAIRNPSAPDSQHIVRTILGVNEEEQSLIFAGD
                     LPKGYYAQLMRANFDRLIVSANEAGENANDRLFKNGSLNTQPVLAIDISCVGRRLVLG
                     ELTEEETETTLQSLPPKSIQIGFYSYGELSPLATGTCELHNQTMTITTLLESP"
     gene            complement(6161..7042)
                     /locus_tag="VAH76_002190"
     CDS             complement(6161..7042)
                     /locus_tag="VAH76_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1386796.1"
                     /translation="MDIRDIKSFLAVVEYHSLTLAAKKLHVTQSAISKRIRKIEDELN
                     IRLIISEGSRLIFTEAAKQLIPYARQMLSAHQNMVHTLKNSQQFTQSIIIGASVYVSH
                     YVLPGFFKYLKETHPTIQAHLKTISEKEVEYILNHGTVDLVICPAREASEKLLSNSLL
                     WEEKLNLVVAINHELAHRTSALSLSELSEYPAILTEKGAALRDKLDTLFEKNQLILNL
                     GFEMSTIDAIKSLVEYGLGWSYLPDSLLSEKLKILKIKDLELTIPFHAFYLKKRISDR
                     LINDLLCDFKKWQNRDN"
     gene            complement(7264..8448)
                     /locus_tag="VAH76_002191"
     CDS             complement(7264..8448)
                     /locus_tag="VAH76_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1386797.1"
                     /translation="MHLKRTLSLTLLAFYGLGTILGAGIYALVGEVAKEAAQFTPLSF
                     LIASIIALFTAISYAELSSRLPHSAGSALYVRRAFHQKWLSGLIGWIVVLTGIISAAT
                     LSHGFVKYFQLFLPLPPSLIITILTVILAGVAIWGIRESATLILFMTLMEVGGLLMII
                     YYGRYSLASIDIKSFSFPHSFDGVLIGAFIAFYAFIGFEDMVNTAEETINPEKTLPKA
                     IFLALISATILYIAVAWVIVLTIPSNTLVKTDMPLIEIIKLQGQSPLLFSIIALISIT
                     NGILVQIIMASRLIYGMANQQNAPQIFSSVYSKTQTPVYSTLLVFLMILLFAYALPIT
                     TLAKLTSSIILCIFIIIHASLIKIKLTEKKPTNAISFPIVFPMISILLTLAFLIIQFF
                     LK"
     gene            complement(8516..9292)
                     /locus_tag="VAH76_002192"
     CDS             complement(8516..9292)
                     /locus_tag="VAH76_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386798.1"
                     /translation="MSEREWPANDYAIGSYIQATVAEKYLAYLKVNPTDKILDIGCGN
                     GAFTKNILLQVPQGSVLGIDASENMLHLAQDMSKKFPNFSVQKADVLTMTFNSQFDYV
                     VSFWCLQWACANIQKAFFNIINALKTGGKFLTLFPAGDDPFIMSYYALRKSGQFASLH
                     NFIPPVDYSHLNNLEKKLESLTCRDLNVKLCRQSITLPSLDTFRKFVNGIAFYQGQLP
                     NEEIKQINEAMVRYYDSECQTKYHGEYQFNFTIYLVTGEK"
     gene            9500..10711
                     /locus_tag="VAH76_002193"
     CDS             9500..10711
                     /locus_tag="VAH76_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEO1386799.1"
                     /translation="MFSQSQQKIVCVLYDDPKGGFPPNYARESIPELKQYPDGQCLPT
                     PDSIDFIPGEMLGSVSGELGLRQFLESNGHQLVVTSDKDGSDSVFARELKDAAVVISQ
                     PFWPAYLTRDRIESAPKLKLAITAGIGSDHVDLQAAMEHNITVCEVTYCNSISVAEHT
                     VMMILALVRDFIPQYNTVIDGGWNIADCVSRSYDLEGMQVGCVAAGRIGLAVLRRLKP
                     FAVKLHYTDRHRLPLHMEQELNLTYHPSVESMVKVCDVVSIHCPLHPETEYLFDDRLI
                     KQMKRGSYLINTARGKICDQHAVADALESGHLAGYAGDVWFPQPPAKNHPWRSMPNHA
                     MTPHTSGTTLSAQARYAAGVREILECWLGNKPIREEYLIVSQGRLAGVGSHSYSAGNT
                     TTGTEQAVELV"
     gene            complement(10817..11941)
                     /gene="ceg10"
                     /locus_tag="VAH76_002194"
     CDS             complement(10817..11941)
                     /gene="ceg10"
                     /locus_tag="VAH76_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg10"
                     /protein_id="HEO1386800.1"
                     /translation="MFTKSCAKATDQNRQELLMDSLLGDFQKMKEKALNQQQEKRSKK
                     REFNSLSSEKTKKPNQYAALTHSQVQEVKAKARTVNDKFHLNAEEKKLWELILLGNQL
                     AQNISSCDLPTDNEDDASLVKLTQIFADETLERTDLTWLNKILKIALYSRGSGFGNCQ
                     EKAFFAFALLLHQAQKPESLIHSLRLATFNNHFILIVNEQFLMDPWLNLAFPLSKGNQ
                     QLEIGYVFEGFGRLVNYFSINQEGQCFTHAVREGDTTERDLSSEKDMANCIHSLLDHR
                     DYFDLSIVSEREIHTKTKRRKYETQEQEVPQPRQETSNQPKETVYTDSNHNTWDNEGA
                     ADDFLEHFEQTHPVYNPGFFSFGQDDVGFKPDLPKNLFKF"
BASE COUNT         3512 a         2238 c         2444 g         3864 t
ORIGIN      
        1 tgtatattca actaatgaat ttttgctatc ttaaagggga tttgtttgga gcgggtgcaa
       61 tttatacggg tttaatagct aataacctcc ctaactctat tgatcggaag caattgagct
      121 ccgagagtcg cctgattttt aaagattgtg aagaaaaatt taagcgatta ttcaatctct
      181 caactacata caatgaacac actaatttaa aaagacaatt caaaactgct ttgatacccg
      241 cattaccgtc acttctcacg gttcagatat atactaaaga cgcttacgat aactctgtca
      301 gcgattttga aagagttcag gagaagttac gtcagttgga tatagaacat aatcggtttc
      361 ttaatcaatt acaaaatgac cattcaacct ggtctaaagc ttattatgat tatatgagta
      421 atgtttatct cccgcaaagt atattcgaat acacgactct tttaagagag tatgaagaac
      481 ttatcacaac gcatggtggc cctattgcga aacagtttca tgaggataag aattcagaac
      541 atctggtaag ctttgttgaa tcgacgctat tagaaaatca atcagatctc aaattatctc
      601 cttttcaaaa tgagagaatt gttgaaatgc tttccgagat tgacgtggta aaaagtgagt
      661 gctcaagccc agatgtgttt gatgacatct cctggaagtt gagagaaatg aattgtactt
      721 tggtaaaagc caaatctatt ggtgattata cttccaaaat ttttgcaaat cttgatactc
      781 catacatgga agaaaatacg gatgaagatc cgaaaccagt gcttgtagaa gaactgcaaa
      841 cggatgatgt gtaccaactt caccatagtg gtatgaaatt gatacgtgag ctcgaacagg
      901 ctatagaaga tcataaaccg aaggttattt atcattgttc aggtgaggca aactatggtt
      961 tctttaagga agccagaagg agggggaggc atttgagtcc agaaccaatg gatatcgaag
     1021 tcatggatgt ggatgtgatg gattatgatg agaacgattt acgtaatcaa accgttatga
     1081 ttacggtgta gtttgtcttg catctataat tgcaatccat cggatagctc ttatagatgc
     1141 caatgcgatt tttaccttat tttgtcctat ttccttgcag aagtttttct atctcttctg
     1201 gtggtatagg cttactcatt aaaaagcctt gaatgtcatt acaccgcctt ttcttgagga
     1261 atttaagctg gttctgcgtt tcgactcctt cggcgatgat attaaaattc atgctttgtg
     1321 cgatagcaat aatcgcatcg atgatcgctt catctcctgg cgattttgaa atattctgaa
     1381 tgaatgattg gtctattttt aggctatcaa cagcgacctg ctttaataaa ttcaggctgg
     1441 aatttcctgt gccaaaatca tccaaaataa tcaacacacc gagtgatttc aattttttga
     1501 tcgttctttg tatttcttta tcaataatca cattttcagt aatttccagc tccagacaat
     1561 gaggttcgac tgaatattct tttaaaagat cctccaccat tgcagggaag tcgcgttgtc
     1621 ttaactgttt acttgctaca ttacaagcga cacgaatttc aggcagcccc attgtgcgcc
     1681 acgcaccaat ttgcttacag gtttcccgca aaacccactc tcctatcggt acgataagcc
     1741 ctgaactttc ggcagtagga ataaaatcca agggtaaaat aatacctttt tgcgggtgat
     1801 tccaccggat taatgcttcc atggaacgaa tctcatgagt attaactgta aattgaggct
     1861 ggtaaaggag gaaaaactca tgatggacaa tcgctttttg caagtctaat tccagctgaa
     1921 attgctggtt tgtttttttg ttgagtttct ctgtataaaa tgagaattgg ttaccacctc
     1981 gaaattttgc ctgatacatc gccatatccg cattactgag taaagtgttt actgttttgc
     2041 catccgttgg ataaacgcta atacctatgc tgacatgaat attgatgtct ctttcagcaa
     2101 tatgaaatgg tttttgaaag gatttgagga ttttttgagc tactccggca atttgctctt
     2161 cagaattaaa cgattgaaaa agcattacaa actcatcccc accaagtctg gcgagagtat
     2221 cttcttttcg caccaaagat cgtaatcgtt cagcaacggc acacagtaat tgatcgcctg
     2281 cttcatggct caagctgtca ttgatcattt taaagcggtc caagtcaaaa aataatatgg
     2341 caaatttcat tttatggcgg gcatagccac tgatagcatg ttcaatacga tcaattaata
     2401 acaagcgatt gggtaaattg gttaatgcgt catgagtggc ttgatactcc agtttttcct
     2461 ttaatcgcgt ttgttcagtg atgtctctga agctccatac acggcctacc gtttgcagac
     2521 caacgcgatg cggtttggaa cagcattcaa ccactttacc atttctaaaa tgataaattt
     2581 gcatgctgga atcatcgatg tgaatagaaa attctttaac atgttgggca tgctgttctg
     2641 aattgagtaa ttgagttgac atatactgaa tgagttgggg ctcactggtt tcttttttta
     2701 tgatggattc gggtatattc cataatttga cgaattgaga gttgtaatca acgatttttc
     2761 cttgtaaatc gacaaccaga atgccatccg aagaggactc aatcgttgct ctcaataagg
     2821 agaaggattg ctgcaaagat tcggttcgtt cttctaccaa ttgatttaaa aattcagtgt
     2881 gtttttgagt ttccctggca agtacccatt tttgtgtcaa agcacttgcc aactgtctta
     2941 cggcaatcac atcaaagggt ttttttagca ccaataaatt atcacttaaa cccagggtat
     3001 tcactgtgct ttcccatgag taatcggaat aggcactgca tattacaatc tgtatttgcg
     3061 ggtcaatggc ccaaatgcgc ttgatggttt ctaccccatc tatccccggg ggcattctta
     3121 tgtcaacaaa ggcgagtggg tatggccgtc cttcttcaag tgaacgtttc actttttcta
     3181 tgccctcaat gccttgagtc gctacatcga tttcaaattc aggcaaagaa atgccaagtt
     3241 gttctgtctc atcatcaaac agctcggcat caaatttatt tagtaaagca gagttcttgc
     3301 tgacatttaa gattttcata aaatcctgat ggattgccgg gttgtcatca atgaccagaa
     3361 tgcgaaaaga ttcagtactc atttctacct cggacttaag gttttgggat tgatgggcaa
     3421 atctaataca aattgagctc cttcgccatt gccattgctt tcagcataaa gtgtaccacc
     3481 cagatctctc gcataaattg cggaacagtg cagtccaaaa ccgtgaccat ttttcttggt
     3541 tgtaaatcca aaagagaaaa ttcgatccag gttatctttt tgaattccaa ttccattatc
     3601 atttatttta atcatgatat gatttttttc agttctccca atggaaataa caattgtcct
     3661 ggaagcattc gcttcgtgta acaaaacaga atcttttgca ttagcgagta aattagtaag
     3721 aatttgaaac aatttggatt tatcagtgat aaactcattg atatccggtt caatctcaat
     3781 acggacatca atttgatgtt tgtccaatat gactgatttt accgcattaa gaatcacatc
     3841 atggattgaa attttctctg ttaaattcga taagccgctc aatgattgct gcatggctat
     3901 gatttcttta atatgattaa tgttagtttc cagactattg gcttctttca tgttttcttc
     3961 atactcttca tgcagggttt tcgataacgc gtccaaatag ggaagaacta atttaccttt
     4021 tggattattt aacaagtatt cgcttaattg attgatgttt tcgtcaagca tggtttttat
     4081 agcggacagt tttttataat gaattttgga tatattcttt ttcagaataa tggcagaaac
     4141 attgacgctg tttaatatat tgcccacatt atgaagtatc gaggtggcca cttccgacat
     4201 accggcaaga cgtgctgttg aaaccagctt attatttagt tcaagaactt gctcttcaat
     4261 aattttcctt ttggagatat ccatcaaaac cccggaaatt tcatttttat gaggattggc
     4321 tcttgctatc gttcggaacc acttataatc attgtcttgg tttcttactc tgagttcata
     4381 atcataatct tctttttctt tgagagcctt ttcgaccact ttttttaatc ccgctatatc
     4441 attaggatgc acaagctcta aaaatgaaga caagttcagc actacattac taggcggcaa
     4501 acccagaatt ttaaatagcc ctttggacca tgttgcggta tctgtgtccg cgtcataatg
     4561 ccaatacccc atttgagcta tatcctgggc gtattcgagt ttcgaattga tatccctcat
     4621 ttctttagaa gaaatatcaa tagagcgctc taataaatat cggtcttgat cattttcgta
     4681 gtatgtttta ttgatatgtg caataaattg agtccaactc tccagatttt gtggtaatcc
     4741 atcagcagta aaaccaaatt tttttaactg tcgctgcaac aaccgatgca tttctgtcat
     4801 tattactgaa ttcctctttt tgatttatgg actctctaac agggtcgtaa tggtcatggt
     4861 ttgattatgc aactcgcagg tacctgttgc cagaggtgat aattcaccgt aagagtagaa
     4921 gccaatttgt atcgatttgg gaggcagtga ttgtaatgtt gtttcagtct cttcctccgt
     4981 aagttcacct aataccaatc ttctaccgac acaactgata tcgattgcca aaacaggttg
     5041 tgtattcaag cttccatttt taaaaagtct atcattagcg ttttctcccg cttcattggc
     5101 actgacaatg aggcggtcaa aattggcacg cattaattga gcatagtatc ccttaggcaa
     5161 atcgccagca aaaatgaggg attgctcttc ttcattgact cccagaatag tgcgcacaat
     5221 gtgttgagaa tctggtgcag atgggttacg gatagcaagc gggtaaagca agcctgctgc
     5281 cggtaattca gaagccctgt caccaagata agttttatag agttttagcg cgggctggtt
     5341 gtccagttca tagagaatat ttgctttaga ccgtgtaatg attctttctg gtccaaaaat
     5401 atcccaacct cctcttgaac catgtcctac atggatttga ttaccgtaaa accctactgc
     5461 cccaatgcaa tggtctacaa tttttccatc gtaaataatc caggtatttt taaaatctgc
     5521 accatctcct gccagccccc ctgtaataat gacccctttg ccagcatgct ggtttaatcc
     5581 tttaattaat tcggaaccgt tgacatttaa gccatcggac aaaacaaata tgctttgggg
     5641 attttgcttt tgatgcaatg attttgataa atgaattcct gcctcataag attcttccgt
     5701 gcttttaacc tctgtttttg ccaattgaag tttcgttgac tcaaattgaa ctactgcaac
     5761 cgaaatgctg ttatcaaaaa tattaggccc aaaaatttct cctgcagtgg agcatcctat
     5821 gatttttgat tttttatagg cttgagccaa ttcttcaatc ggtttgctgt ttgccctaaa
     5881 gatggggctg gcaaaaacta aaattaaggt attttcggag tcgagtgcag gaaatgttgg
     5941 aatactccat ccctggtttt gaatgtattg aaatgattca attttcatca tgttgctgtg
     6001 tctcggattt atcttatgat taattatagt atatgttcaa aaggatcaaa gaagcggaga
     6061 ttcagtctat tgcaatgtta gtgttgtaaa aacgtattaa caacggtctg ttgtttcgat
     6121 tttgataaag acggttattt tcgtcctgga ttcattcctg ttaattatcc cgattttgcc
     6181 attttttaaa atcgcatagc aggtcattaa tcaagcggtc actgattctt tttttgagat
     6241 aaaaagcatg aaatgggatt gttagctcaa ggtcttttat ttttaagatt tttaattttt
     6301 ctgacagtaa actatcaggc agataactcc agcctaaacc gtactctact agagatttta
     6361 tggcatcgat agtgctcatt tcaaaaccca ggtttagtat cagttgattt ttttcaaata
     6421 gggtatcaag cttgtcacgc aatgccgctc ctttctcagt taagatggcc gggtactctg
     6481 atagttctga gagtgaaagt gcggaagttc tatgggctag ctcatgattt attgccacaa
     6541 ccaaattcag tttttcttcc cagagcaatg agttgctcaa taatttttca gatgcttccc
     6601 gtgccgggca gattaccaga tcaactgtgc cgtggtttag aatatactcg acttcctttt
     6661 cactaatggt tttcagatgc gcttgaatgg tcgggtgggt ttcctttaag tatttaaaaa
     6721 agcctggcaa tacgtagtga gacacataca cggaagcccc tatgatgatg ctttgcgtga
     6781 attgttgcga gtttttcagg gtgtgaacca tattttgatg tgcggaaagc atttgcctgg
     6841 cataagggat aagctgcttg gctgcttcgg taaaaattaa cctcgagcct tcactaatta
     6901 tcaatcgtat atttaactcg tcctctattt ttcgaatgcg tttgctaatg gcagattgag
     6961 tgacatgcaa ttttttagct gccaaggtga gagaatggta ttcgacgaca gcgagaaagg
     7021 atttgatgtc tctaatatcc atagaatgct tgccttttta caagaagtat agcatagcgg
     7081 cgctaagcgc tttctaaaca aggatggcag atgatcataa tgttctaagc aggggtcgag
     7141 ggatcatgcg gcattttaaa cgagggatac aggcacagag gagcagcgaa ttgaaaacag
     7201 acaatcactc tgttgattcc caaaatttat atgacaccgg gttaaaaatt tatgagaaat
     7261 aatctacttt aaaaaaaact gtatgattaa aaaggccagg gttaataaaa tcgaaatcat
     7321 cgggaaaaca atagggaatg aaatggcgtt tgttggtttt ttctctgtca gtttgatttt
     7381 aattagcgag gcatgaatga tgataaaaat acacaatatg atactgctgg ttaatttggc
     7441 tagggtagta atagggagag cataggcaaa aagtaaaatc ataagaaata ccagtaaggt
     7501 cgagtagact ggtgtttgag ttttggaata gacagaagag aatatttggg gggcattttg
     7561 ttgattggcc ataccataaa taagccgtga agccataata atttgcacca gaattccatt
     7621 ggtaatagaa attaatgcaa tgattgaaaa caacaaggga gattgtccct gaagcttaat
     7681 aatttcaata aggggcatgt ccgttttgac taaagtattg gatggtatgg tcaagacgat
     7741 aacccaggcg actgcaatat ataaaatggt tgcggatatt aaagcaagaa aaatagcttt
     7801 tggaagtgtt ttttcaggat ttattgtttc ttctgcggta ttaaccatat cttcaaaacc
     7861 aataaaagca taaaaagcaa taaaagctcc aataagcacc ccatcaaaag aatgagggaa
     7921 agagaaggat ttgatgtcga tagaagctaa tgaataacga ccgtaataaa taatcatcaa
     7981 taatccaccc acttccatga gcgtcatgaa caggataaga gtcgctgatt cacgaatacc
     8041 ccaaatagca accccagcta aaatgacggt taatatagtg atgatgagag aaggtggtaa
     8101 tggcaaaaac aattgaaaat atttgacaaa accatgggat agggtggctg ctgaaatgat
     8161 acctgttaag accacgatcc agcctattag gccagatagc catttttgat ggaatgccct
     8221 acgcacgtaa agagcagaac ctgcgctatg aggtaacctt gagcttaatt ctgcataaga
     8281 aatggcagta aaaagagcga tgatggatgc gattaagaat gatagagggg taaattgtgc
     8341 cgcttctttt gcgacctcac cgacaagggc ataaattccc gcgcccaata tagttccaag
     8401 cccataaaag gctagcagtg ttaatgataa agttcgcttt aagtgcatcg ctcccccatt
     8461 ttatctggag atctatagtc gtagaacgga ttttcttttt tatttgctac ctcttttatt
     8521 tttcgccagt taccagatag atagtgaaat tgaattgata ttcaccatga tactttgttt
     8581 ggcattcgct atcgtaataa cgaaccatcg cttcgttgat ttgctttatc tcttcattgg
     8641 gcaattgtcc ttgataaaaa gcaatgccat tgacaaattt cctgaaagta tccagggatg
     8701 gcagggtaat ggattgtcgg catagtttca cattaagatc acggcaggtg agtgactcca
     8761 gttttttttc gaggttgttt aagtgagaat aatccacggg agggataaaa ttatgcaagg
     8821 atgcaaattg tcctgatttt cttaaagcat aataactcat gataaacggg tcgtctcctg
     8881 ctggaaaaag agtgagaaat tttcctccgg ttttcaatgc gttaatgata ttaaaaaatg
     8941 ctttttgaat attggcgcat gcccattgca agcaccaaaa agagacgaca taatcaaact
     9001 gtgagttgaa tgtcatagtg agtacatccg ctttttggac tgaaaaatta gggaattttt
     9061 tactcatgtc ttgggctaaa tgtagcatat tctctgaagc gtctatgcct aatacagagc
     9121 cttgaggcac ttgcagaaga atgttttttg taaaagcgcc gttaccgcat ccaatatcca
     9181 atattttatc agtgggattt actttcaggt acgctaaata tttttcagcc acagtcgctt
     9241 gaatataaga tccaatggca tagtcattgg cgggccactc acgttctgac atacttattc
     9301 ctttcttttg agatggttat ttattccata attgttcagg tcatcgttgt tcatgataca
     9361 tcatggacag cccctaaaat agatcatgaa ttcttttatg aatagtattt ttattgagat
     9421 gaatgaatta aatcaagtat agtgagcgtt gtaaaaaaat caggatgttt ttacttcaaa
     9481 ctatgaaaag gagtcaggta tgttcagcca gagtcagcaa aaaattgttt gtgtgcttta
     9541 tgatgatcct aaggggggat ttcctccaaa ctatgcaaga gaatccattc cagaattgaa
     9601 acaatatcct gatggtcaat gtttacctac tcctgattcg attgatttta ttccaggtga
     9661 aatgctaggt tctgtgagtg gtgaattggg actaagacag tttcttgaaa gcaatgggca
     9721 tcaattggta gtaacctctg ataaggatgg ctctgattca gtatttgcca gggagttaaa
     9781 agacgctgct gtcgtcattt cccaaccttt ttggccagct tatttaactc gtgatcgtat
     9841 tgaaagtgca cctaagctta agctcgctat caccgcaggt atcggctcgg atcatgtcga
     9901 tttgcaagcg gccatggaac ataatatcac cgtttgtgag gtcacttatt gtaacagcat
     9961 cagtgttgct gagcataccg tgatgatgat tttagccttg gtgcgggatt ttataccaca
    10021 atacaacacg gtaattgatg gcgggtggaa tattgcggat tgcgtcagtc gctcttatga
    10081 tttggaaggc atgcaggtag gatgtgttgc tgcaggccga attggattgg ctgttttaag
    10141 acgtttaaag ccatttgctg taaaactgca ttacacagac cgacatagac taccactaca
    10201 catggaacag gaactgaatt tgacctatca cccatctgtc gaatccatgg tgaaagtttg
    10261 tgatgtcgta tcgattcatt gtccattgca tccggaaaca gaatatcttt ttgatgatcg
    10321 attaataaaa cagatgaaac gaggctctta tttaattaat accgcccgtg gaaaaatatg
    10381 cgatcagcat gctgtagcgg atgctctgga atctgggcat ttggctggtt atgccggaga
    10441 cgtttggttt ccacaacccc cagccaaaaa ccatccttgg cgatcgatgc caaatcacgc
    10501 catgacacca catacatcag gaactaccct gtccgcgcaa gcgcgatatg cggcgggagt
    10561 cagagaaata ttagagtgtt ggttagggaa caaaccaata agggaggaat atctgatcgt
    10621 gtctcaaggc cgcttagccg gggttggttc tcattcttat agtgcgggta atactactac
    10681 gggaactgag caagcagtag aattggtttg attcctcgtc gcttatgctg tcttatgtct
    10741 ttctgttttc attcaaagta gccgctgcct ttaaagacag cggcagttca gggaaaaggg
    10801 gcaaacagtg gatgatttaa aatttaaata aattctttgg aaggtctggt ttgaaaccaa
    10861 catcgtcttg gccaaatgaa aagaaacctg gattgtagac agggtgtgtt tgctcaaaat
    10921 gctctagaaa atcgtctgcg gctccttcgt tatcccaagt gttatggttt gagtctgtat
    10981 atactgtttc tttaggctga ttggatgttt cttgtctagg ttgaggcact tcttgttcct
    11041 gagtctcata ttttcttctt tttgttttgg tatgaatttc tctctccgaa acgattgata
    11101 gatcaaaata atccctgtga tcaagtagcg aatgaataca gtttgccatg tctttttcag
    11161 atgataggtc acgctctgta gtgtctcctt ctcgcactgc atgggtaaaa cattgacctt
    11221 cctgattgat tgaaaaataa ttgacaagtc ggccgaatcc ctcaaaaacg taacctattt
    11281 ccagttgttg attacccttt gataagggga aggccaaatt caaccaggga tccattaaaa
    11341 actgttcatt gactattaat ataaaatggt tattaaaggt cgctaatcga agggagtgaa
    11401 ttaagctttc tggtttttgt gcctggtgta acagaagtgc aaaagcaaaa aaagcttttt
    11461 cctgacaatt accaaaaccg gaacctctgc tatacagagc aatcttcaga attttattta
    11521 gccaggtcaa atcagttctt tccagagttt catcagcgaa aatttgagtt agcttaacca
    11581 gtgaagcatc gtcttcatta tcggtgggta aatcacagga actgatgttt tgtgccaatt
    11641 gattgccaag cagaatcagt tcccatagtt tcttttcttc agcattgaga tggaatttat
    11701 cattaactgt gcgggctttt gccttaactt cctgaacttg agaatgagta agggcagcat
    11761 actgattcgg tttttttgtt ttttcagaag ataagctatt aaactctctt tttttggagc
    11821 gtttttcttg ttgctgatta agtgcttttt ctttcatttt ttgaaaatca cctaaaaggc
    11881 tgtccataag taattcctgt ctattttgat cagtcgcttt ggcacaggat tttgtgaaca
    11941 tgttacactc tctttataat agttaattac gatatgacaa gttgaacctc tgatgataat
    12001 gagctggact gctgtaaaaa cagagcctgg ttcactgaga gtgctatttt ataagtta
//
LOCUS       DATNSN010000106        11631 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.106, whole genome shotgun sequence.
ACCESSION   DATNSN010000106 DATNSN010000000
VERSION     DATNSN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.106"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(64..1869)
                     /locus_tag="VAH76_002195"
     CDS             complement(64..1869)
                     /locus_tag="VAH76_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1386801.1"
                     /translation="MNGHLLLNVFIFLAAASIMVPLASRFRLGSVIGYLTVGMLIGPF
                     GLKLIGNAEQIMHFGEFGVVMMLFLIGLELEPIMLWKLRKLIVGLGGLQVLLTTIALS
                     SAAVMMGYNLQASVAVSMALALSSTALVLQMLQEKNLLKTAEGETSFAVLLFQDIAVI
                     PILVILPLLGNHNGINVAINPHTAASIAQLPKWLHAIVVAGIIGSIVLAGHYLSRYLF
                     LIIAKTNIREVFTAFSLSLVVGITLLMETIGVSPALGAFIAGMVLANSQYKRTVEADI
                     QPFKGLLLGLFFISVGMGINFNLLFSQMAVIIPIVLGLITIKALILFTLGKIFQLTKI
                     QALGFALGLSQGGEFAFVLFQYSSTLKVISPEISGFFTLVVALSMLATPFLMLLYHRF
                     MMPKLISKLPEREYDSIHEKNNIIIAGYGRFGQIIGRFLSGENIKVTVLEKNPEQVEL
                     LRKFGYTGYFGDANRLDLLKSAGAAHAQLLIVTVGNPDINLEIVKLAKEEFPQLKIFA
                     RARNRRHAYELHKAGVHYFKRELFDSSLTMTKEILKFLGYKPEEIEKKANAFQKHDEE
                     TLIKSFDFFEEEASLINFSRQAKGELERILQNNNP"
     gene            complement(1889..2368)
                     /locus_tag="VAH76_002196"
     CDS             complement(1889..2368)
                     /locus_tag="VAH76_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEO1386802.1"
                     /translation="MTLCPCGSQNKYEQCCGIYLESKKAPETPEQLMRSRYTAYSLAK
                     IDYIKQTMQGKPLIAFNETEAKQWAQNVSWLGLKIIQSYMERPDKGFVEFIATFLDGN
                     QLKNIHELSEFQKDNSVWFYVDGEAKQLPNKQDKQKIGRNSPCPCGSGKKFKNCHDK"
     gene            complement(2631..3134)
                     /locus_tag="VAH76_002197"
     CDS             complement(2631..3134)
                     /locus_tag="VAH76_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386803.1"
                     /translation="MPKKFTRKIPLISREECFALLPGYSINAMNLLKRGNHNPYYEEI
                     ESLSKIFVALITHYSEPIESIINCMEKAMYDAYKEELDKIEHEIQRLFKIRKNTPIHS
                     LFGEREIEKLTIQISELEKCKSVSVKEIIQGVIYDTLDKASQTLVDRIPGNYKLQEYE
                     SRGNPFQ"
     gene            3336..4427
                     /locus_tag="VAH76_002198"
     CDS             3336..4427
                     /locus_tag="VAH76_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386804.1"
                     /translation="MFKRKQIVKNDSDMDYVDDFISIEEHSSGDDVTNELSLAEQVFT
                     RLQIYSEGFKPSFFIHKDLANLSLTSKTLNGVVCSELNQRAAQKLLTHVVKGEQDKAQ
                     AMLEKNPGLLLIKSKAVDYSGRTIVGTAFQGAIGAGDKPMWKMMLSYFEGLEQGEALR
                     QFDEQFPSGTEETPAAELKAYYDAIASAIIHDEDHGLSVIEGFRKEITCQKEITQGKH
                     FNLQHLIAAYQAYIDNFYAFGNWGNRDLFWQKVIGYVQRQMTAYDAQIYCSGVISVLD
                     KESSFSRTFELSDDKNFFPLQIDSALGFDFACYSYYSARALDSGSHHVLPSPLNEYWI
                     WIEKLCGEKTDALVGLRESLEQGVYYQLK"
     gene            4708..4818
                     /locus_tag="VAH76_002199"
     CDS             4708..4818
                     /locus_tag="VAH76_002199"
                     /inference="COORDINATES: protein motif:HMM:NF017416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1386805.1"
                     /translation="MFLLQQLFNKTDPQIEYDVKDNAAYQLFCGIGIVEQ"
     gene            complement(5017..6297)
                     /locus_tag="VAH76_002200"
     CDS             complement(5017..6297)
                     /locus_tag="VAH76_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO1386806.1"
                     /translation="MELTTTENQLFKSSKVSLALVTVLVGIGSGFLGMCLAMLLHYLQ
                     HIAYGYSPLHIISNETFLEGVRASSSERRISILFLCGLIAGMGWWALYRFGKPLVSIA
                     TAIKSGKPMPVLSTFIHAFLQIITIALGSPLGREVAPREVSSVFAYWLSSKAGLTPEE
                     SKIMLACGAGAGLAAVYNVPLGGAVFVLEVLLCTSNWTVLLPALSSSAIAVLVSWWGL
                     GNEAIYQLPEFSLSAPLIIWSILASPVLGFFAFWFIQIATIQRSKAMHNWQMILACLI
                     NFLIIGLFAIYFPSLLGNGKSPAEMEFDQSVGIGLSIILFMLRNVFVWSSLRVGAQGG
                     LLTPSLANGALLGAILGGIWNLYLPPAPIHAFALIGATAFLAAAQKMPLTAIILIFEF
                     TRINFIFLIPIMLAVSGSIAMCQLTKNYYIKYKV"
     gene            6774..8054
                     /locus_tag="VAH76_002201"
     CDS             6774..8054
                     /locus_tag="VAH76_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386807.1"
                     /translation="MNTLSFKHPLLAKTYLWIIWTIAACFFFYKYLIQVSPSVMTADL
                     MKAFQVNAAGLGNLSAFYFYSYLFMQIPVGLMLDRYSPRLLTTLAIFVCSISTYIFSQ
                     TNTLWLACLSRALMGAGAAFAAVSCFKLAAVWFSPKRFALVSGMFMTAAMLGAVGGQM
                     PLAFLVQHEGWRKALELVSVMGIVLGVVYFLVLRDKPAQTQPTQNSKEPVVQLLRRII
                     VNKQAWALSLYSGLAFAPVSVFGGLWGVPFLEKAYLLSRTDAALAISFIFVGFAAGAP
                     FWGWFSDFIRRRKPVLFTGTCSALLCLLVVIYSSNQNLLTLIILLFLFGFGASGFFTS
                     FAMIRELFPLVLVATVLGIMNTFNSVFEALFEPLVGALLDWTWEGTVVDGIHQFTLHG
                     YYFSLLLLPLSLILALLTLLLIDETYCRTKEEVE"
     gene            complement(8064..8483)
                     /locus_tag="VAH76_002202"
     CDS             complement(8064..8483)
                     /locus_tag="VAH76_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386808.1"
                     /translation="MCNWNRIVGLALMGFSAFLQAKTYIPQCPKEINTLERIQTSPDG
                     WETLSGIKNNYLGNVSFYSGHPKGQASLKPNRINKKKAEWKFSTHDTIYIVCHYNQTG
                     IELTQQLPQKTKGCTVIFNPNAKGPYGFLPQKITCNQ"
     gene            complement(8537..8803)
                     /locus_tag="VAH76_002203"
     CDS             complement(8537..8803)
                     /locus_tag="VAH76_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386809.1"
                     /translation="MESMIDLVGYVASSLVFATFYVKKILTLRLIAVCSNVAFITYGL
                     GYHLHPIFILHSVLLPLNLYRIFELLKNDPEFTILARINLKATR"
     gene            8915..10636
                     /gene="legLC8"
                     /locus_tag="VAH76_002204"
     CDS             8915..10636
                     /gene="legLC8"
                     /locus_tag="VAH76_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC8"
                     /protein_id="HEO1386810.1"
                     /translation="MIFELNILPNRNFLFIFGDNMPLTSLKKFLKDIGKENIKQHQVK
                     LVLKTSQDIQLFLNALRDSRNHGISSLSELDLSDTRFTNQELSDLVTALNNIPGIKSL
                     RLDSCGLKDSDTVELSKLTHIKKLSLKSNYLKNRPMFNSMLEALYLDYNTELSASYVL
                     FSLSRNAAALKKLSLRNCGVTDANLEYLTRPESRLKSLTHFNLRRNNITHQGVDSFAH
                     LQSLTTIDLSQNTGIGDEGVSRLAPLKQLRTLYLDNCGIGGEGIKAIAKMNLQTVDLS
                     FNPGLKKEWGLDDIRPNHTIRTLLLTFCSLNDNHAKLIVSKFPAATDLNVANNNMTRA
                     GVKTLLSNPIIENLDVSTQSLYAKSTGASGTGMISPRIMMKKKEIKQQEKEKVLDLLD
                     TICNTITLKSINLEHTGITSRMLLSLIPDETDHKRYLKKINGVSCKELKPKLEQQIAL
                     RKAEKQSSPPVIIAVEDSKPSDLMIKSGSSSKTEAQTREDHLIANPDEKIKALELENQ
                     KLKDELAIARKRIFELENQSLSGSKTRKKTSIPPLAIPVMFQDFAKPGKNETDENTSN
                     SITAIPK"
     gene            complement(10700..>11631)
                     /locus_tag="VAH76_002205"
     CDS             complement(10700..>11631)
                     /locus_tag="VAH76_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1386811.1"
                     /translation="SFVEKIQNAGGKPIYDIPVAEGRAIFDQLQALPNEKPDVDVEDK
                     TLPVGPGGKVDIRIVRPKGAKEALPVIMFFHGAGWVFGDYQTHGRLVREIAVGTHTAV
                     VFVKYTLAPEAQYPTQIEESYAAMKYIAEHGKQFNLDTSRFVAAGDSVGGNMTSVMTL
                     LAKERGGPKIDYQVLIYPVTDANFDNASYKEFAEGPWLTKKAMEWFWDKYLPNKEKRK
                     EITASPLQASLEQLKGLPPALVITGECDVLRDEGEAYAHKLNAAGVAVTAVRHLGTIH
                     DFLMLNDLSETPACRNAVETIIGHLTHIFGKKK"
BASE COUNT         3547 a         2145 c         2305 g         3634 t
ORIGIN      
        1 aatttcaggg gtggatcgga gatgtttgta ctttaatgag agatcatacc ttttatgatt
       61 tgattatggg ttattatttt gcaaaatacg ttctaattct ccttttgcct gacgtgagaa
      121 attgatgagg ctggcttctt cctcaaaaaa atcaaaagat ttaattaatg tttcttcatc
      181 atgtttttgg aaagcattgg cttttttctc aatttcttca ggtttataac caagaaattt
      241 taaaatttcc ttggtcatgg ttaacgaaga gtcaaataat tctcttttga aataatgaac
      301 tcctgcttta tgtaactcat aggcgtgtcg cctatttctt gctcgggcaa aaattttcaa
      361 ttgaggaaat tcttctttag ctaatttcac aatttccaaa ttgatgtcgg gattaccgac
      421 tgtgactatg agcaattgag catgggctgc tcctgcgctt tttaaaaggt cgagacgatt
      481 cgcatcacca aaatatccgg tgtagccaaa ttttcttaat aattcgacct gctccggatt
      541 tttttccaaa acggttacct tgatgttttc accactaaga aatcgaccaa ttatctgacc
      601 aaatcgaccg taaccagcga taataatgtt atttttttca tgaatagagt cgtattcacg
      661 ttctggtaat ttgcttatta atttcggcat cataaaacga tggtacaaaa gcattaaaaa
      721 gggtgtggcc aacatggaca gagcgactac cagagtgaaa aaaccgctta tttccgggct
      781 gattactttt aatgtgctgg agtattgaaa aagaacaaag gcaaattctc caccttgtga
      841 taaaccaagg gcgaatccga gcgcctgtat tttagtgagt tgaaatatct tgccgagcgt
      901 aaagagaatc aatgccttaa tggtaatcaa accgagtaca atagggatga ttacggccat
      961 ttgactgaag agtagattga agttgatacc cattccgact gagatgaaaa ataatccaag
     1021 aagcagtcct ttgaaaggtt ggatatcagc ttctacagtc cgtttatatt gagagttagc
     1081 taaaaccata cctgcgataa aagctcccaa agccggagac accccaattg tttccattaa
     1141 caaggtaatt ccaacgacca gggataagga aaatgctgta aatacttcac ggatattggt
     1201 ttttgcaatg attaggaaca agtatctgga taagtagtgt cccgccaaaa caatgctacc
     1261 gataatgccg gcaacgacaa tagcgtgaag ccacttgggt aattgagcaa tagaggcagc
     1321 cgtatgaggg ttaattgcca cattaattcc attatgattg cccaataaag gcaaaatcac
     1381 caagattggt atgaccgcaa tatcctggaa taagagaaca gcaaaggaag tttcaccctc
     1441 cgctgttttc aaaagatttt tctcctggag catttgcaag actaaagcgg ttgaagatag
     1501 ggcaagtgcc atactgaccg ctacactggc ttgcaaattg tatcccatca taacagctgc
     1561 cgaagacagg gcgattgttg ttaacaacac ttgtaagcca cctaagccaa ctattaattt
     1621 tctcagtttc cagagcatga ttggttctaa ttccaaacca atcaagaata acatcatgac
     1681 tactccaaat tctccaaaat gcataatttg ttcggcattg cctattagct taagtccaaa
     1741 aggtccgatg agcatcccca cggtaagata accaatcaca gaaccaagtc taaatcggct
     1801 tgccagtgga accatgatgc ttgcagcagc aaggaaaatg aaaacattga ggagtaagtg
     1861 accgttcatc tgctaccttg agtttaattc atttatcatg acagttttta aattttttac
     1921 cgcttccaca gggacatggg ctattgcggc ctatcttttg cttatcttgt ttgtttggta
     1981 actgtttagc ttctccatcc acataaaacc aaacggaatt atccttttga aactcactta
     2041 attcatgaat atttttcaat tgatttccat caagaaaggt tgctataaat tcaacaaatc
     2101 ctttatccgg cctctccatg tagctttgaa tgattttcaa tcccagccag gaaacatttt
     2161 gagcccactg ctttgcttct gtttcgttga aagcaattag tggtttgcct tgcatggtct
     2221 gtttaatata atcgattttt gcaagagaat acgcagtata tcttgaacgc attaattgtt
     2281 ctggtgtttc aggagctttc ttgctttcca gataaatccc gcaacattgc tcatatttat
     2341 tttgtgaacc acaggggcaa agagtcattt agttacctgt aaaaactggt ttatgggtaa
     2401 atttacaatc cgttatatta acgaatcccg gattatattc tatacggcat tccattattt
     2461 ctaaactgtt ttgttgcatt ggattttttt ataaaaacag gcacaaagtg taattcatta
     2521 ttaatccaca cagcgatttt aaaatgggta ataaaaaaat gaccaagtgc tatcggagta
     2581 aattaggtta taagtgattg gttgtgtttc atttaacaat gaactgaatc ttattgaaaa
     2641 ggattgcctc ggctctcata ttcttgcagt ttatagtttc ccggtattct atccaccaga
     2701 gtttggcttg ccttatctaa ggtatcgtag ataactccct ggataatttc ctttacagac
     2761 actgatttgc atttttccaa ttctgaaatc tgtatagtca atttttcgat ttctctttcg
     2821 ccaaatagag aatgaattgg agtatttttt cttatcttaa acaggcgttg aatttcgtgc
     2881 tcaattttat ctaattcctc tttataagca tcatacattg ctttttccat gcaattgatg
     2941 attgattcta ttggttctga ataatgagtg atcaaggcaa caaaaatttt tgataaggac
     3001 tcaatttctt cataataggg gttgtgattc cctcgtttca gaagattcat tgcattgatg
     3061 ctgtacccag gtaataaagc gaaacactct tctctggaaa taagtggtat ttttcgagta
     3121 aatttcttag gcatagtgaa catccttttc atcaggtcta cattataatt atagcatcta
     3181 tattattcaa tttgatcaat gtggtctata atttgacata tcccagatag ttagtcacta
     3241 gcgcgcaata gccattgtaa agtgtaatta atgatcatgt cgatcatttt gtgataaact
     3301 ctgccctctt taatagtcta gcaaagtgaa gcgatgtgtt caaaagaaag caaatagtaa
     3361 aaaatgattc ggacatggat tatgtcgacg attttataag tattgaagaa cacagttcag
     3421 gagatgatgt tactaatgaa ctttctttag cagaacaagt atttacccga ttgcaaatct
     3481 actcagaagg atttaagccg agctttttta tacacaagga tttagcgaat cttagtttaa
     3541 cctctaagac attgaatggt gttgtatgtt ccgagttgaa tcaacgagca gctcagaaac
     3601 tcttaaccca cgtagttaag ggagaacaag ataaagcgca agccatgcta gaaaaaaatc
     3661 caggattgct cttaattaaa tcaaaagcgg tagactattc tggaagaacc attgtaggaa
     3721 cagcttttca aggtgcaatt ggtgcgggag ataaaccgat gtggaagatg atgctttctt
     3781 attttgaagg cttagaacaa ggagaagcct taaggcagtt tgatgaacag tttcccagtg
     3841 ggactgaaga aactcctgcc gctgagctaa aagcctatta cgatgctatt gcatcagcca
     3901 ttatccatga tgaagaccat ggtctctccg taattgaagg atttagaaaa gaaataacct
     3961 gccaaaagga aattacgcaa ggcaagcatt tcaatcttca gcacttaatc gcagcgtatc
     4021 aggcctacat cgataacttt tatgctttcg gtaattgggg caaccgtgac ttattttggc
     4081 aaaaagtgat tggttatgtg cagcggcaga tgaccgctta tgacgcccaa atttattgtt
     4141 cgggtgtaat cagcgtatta gataaagaga gttcattcag taggacgttt gagttgtctg
     4201 atgataagaa tttttttccc ttacagattg attctgctct cggtttcgat tttgcttgtt
     4261 atagttatta ttcggcccgc gctttggatt ccggtagcca ccacgtgcta ccttccccgt
     4321 tgaatgaata ctggatatgg attgaaaaat tatgtggaga aaaaacagac gcacttgttg
     4381 ggcttagaga gtcgcttgag caaggggtgt attatcagct caaataattg ttttttattg
     4441 agaccaaact gagcattaaa cagatcctta gctaaaattc caagtcctgc ttgatgggtg
     4501 gtttgaataa actcgattaa tagctgctga taagaccatt gagcatttgt ttttacccac
     4561 caggaacacc actgggacag atagtttttg atcgcttgga tagcaaaccc atgagtttac
     4621 tggtagaaac gattcttcct gatttaaaga agacaaaagg aggatgttgg tggttgggca
     4681 gaaaactaag agttcatatt catttgggtg tttttattac agcaactgtt taataagaca
     4741 gatccgcaaa ttgaatatga tgtcaaagat aacgcggcgt accaactttt ttgtggtata
     4801 gggattgttg aacaatgaca tattcctgat catacaaaaa ttgaagagtt tcgatcacga
     4861 ttaagtgaag aaactcaaga aaccctagaa aatcagatgg caaagcaagc agttttgctt
     4921 ggatttaaca tgaggcaact cattaagtgg caaaaatttc ctgtcaggag aaaaatcgca
     4981 tgaatcaatt tcgcgccaga gactaattac aaacgatcat actttatatt taatgtaata
     5041 gttcttggtt aactggcaca tagctataga gccggaaaca gcaagcatga tagggataag
     5101 aaagataaaa ttaatcctgg tgaattcgaa aattaagata atggcagtta aaggcatttt
     5161 ttgtgccgca gcaagaaaag ccgtggcacc aataagcgcg aaggcatgga tgggagcggg
     5221 tggtaaatac aaattccaaa ttccaccaag aatagctcct aataaggcgc cgttagccaa
     5281 tgatggtgtt agtaagccgc cttgggctcc tacacgcaag cttgaccata cgaatacatt
     5341 cctcaacata aataatataa tacttaaacc aattcctact gattgatcaa actccatttc
     5401 tgccggactt tttccgttac ctagtaaaga aggaaaataa attgcgaata agccaatgat
     5461 taaaaaatta attaaacaag ccaatatcat ttgccaatta tgcattgctt tgctgcgttg
     5521 tattgtagca atttgaataa accaaaatgc aaaaaagcct aacaccgggc tggcaagaat
     5581 agaccaaata attaaaggcg cacttaatga aaattcaggg agttgataaa tcgcttcatt
     5641 tcccaaaccc caccaagaca ccagaactgc tatagcagaa gaagataatg ctggaagcaa
     5701 gacagtccag ttggaagtgc ataacaacac ttccaaaaca aaaacagcac cgcctaaagg
     5761 cacattataa actgcagcaa gccccgcgcc tgctccacat gccaacatga tcttgctttc
     5821 ctcaggagtt aatccagctt tgctagatag ccagtaagca aagacagagc ttacctcacg
     5881 aggagccacc tctcgtccca gaggtgagcc caatgcaatt gttatgattt gtaaaaaagc
     5941 atgaatgaat gtactgagaa ctggcatggg tttacccgat tttattgccg tcgcaatact
     6001 gactaatggt ttaccaaagc gataaagtgc ccaccatccc atgcctgcga ttaagccaca
     6061 aaggaatagt atgcttattc tccgctcaga tgaagaggct ctaactccct ccagaaaagt
     6121 ttcattactg atgatgtgaa gggggctgta accataggca atatgttgaa gataatgcaa
     6181 taacatggct aggcacatgc ctagaaaacc tgaaccgatt cccaccagaa cagtaaccag
     6241 agcgagactt actttggaac ttttaaatag ttggttctcg gttgtggtta actccattcc
     6301 ctggattcct ttaaggtaac ttgtcatcaa tttgcttata ggggtaagaa ttttattatt
     6361 tctgtatgca gatttttttg caagtgaaac agattaataa atgtagccgt aataacaaaa
     6421 tttaaaaaag tatccattgt ttgtttgcga tcttgacagc acattgccta aaaagttaac
     6481 attaaacatt cttttaattt ccagtttttt atgaatataa gacgacaatt aataagcaga
     6541 aagtcttgtt cccaaagctg caacagtcag cctgttggat tgtcgcctat tgttttaaac
     6601 tctctcatct caattgtcgt cgtggtggtg gtcagcttta ccctttcttc tgctctgaga
     6661 cggcttctct cctgacttat ctgccccgcc tcagggcgat aacagcagca gataagacct
     6721 ggcggttttt gaaattttat ctctaacctg aagcaaataa gttaggagtt attgtgaaca
     6781 cattgtcatt caagcatcca ttgctggcta aaacttatct ttggattata tggaccatcg
     6841 cggcatgttt tttcttttat aaatatttga ttcaggtttc tcccagtgtt atgacagctg
     6901 acttgatgaa ggcatttcag gttaatgcgg ctggtcttgg gaatttgtca gctttttatt
     6961 tttattccta tttatttatg cagattcctg tgggccttat gttagacagg tacagtccaa
     7021 gactgctgac aactcttgct atttttgtct gcagtatcag tacttatatt ttttctcaaa
     7081 ccaatactct atggttggcc tgcttaagtc gagcattgat gggggcggga gctgcttttg
     7141 ctgcggtgtc ttgttttaag ctagccgctg tctggttttc tccaaaacga tttgctttag
     7201 tatcaggaat gtttatgaca gcagctatgc tgggtgctgt gggagggcag atgcctttgg
     7261 cattcttggt tcaacatgaa ggttggcgca aggcgcttga attagttagt gtgatgggaa
     7321 ttgtcctggg ggttgtgtat tttctcgttt taagagataa gcctgcacaa acgcagccta
     7381 cgcaaaatag caaggaacct gttgttcaat tgttaagacg tattatcgtt aataagcagg
     7441 cctgggcttt gtctttatat agcggattag catttgctcc tgtttcagtt tttggtggat
     7501 tatggggagt tccttttttg gaaaaggctt atctcttatc tcgtactgat gcagcccttg
     7561 cgatttcatt tatttttgtc ggttttgcgg caggcgcgcc attttgggga tggttttctg
     7621 attttatcag gcggagaaag cctgttttgt ttacaggaac gtgttctgct ctattgtgtt
     7681 tattggtagt tatttatagc tcaaaccaaa atttgcttac tttaattatt cttttatttc
     7741 tctttggttt tggagcgagt ggatttttta ccagctttgc aatgatcagg gaattatttc
     7801 ctttggtact tgtagcgaca gtacttggta ttatgaatac gtttaattcg gtgtttgagg
     7861 ctttgtttga gccactggtt ggtgctttgc tggattggac atgggagggg accgtggtgg
     7921 atggtattca tcaatttact ttacacgggt actatttttc tcttttattg ttgcctctgt
     7981 ctttaatttt agccttattg accttgttat tgattgatga aacatattgt cgaacaaagg
     8041 aggaggtaga gtaaacctcc tcattattga ttgcaggtta ttttttgagg caggaaacca
     8101 tatgggcctt ttgcgtttgg attaaaaatc acagtacatc cttttgtttt ttgaggcaat
     8161 tgttgtgtca gttcaattcc tgtttgattg taatgacaca caatgtagat ggtatcatga
     8221 gtagaaaact tccattcggc ttttttctta ttgatacggt ttggttttaa gctggcttga
     8281 cctttggggt ggccagaata aaaggaaaca ttacccagat aattgttttt aatcccgctc
     8341 agggtttccc atccatccgg agatgtctgt atacgttcaa gcgtattaat ttctttaggg
     8401 cattgaggga tgtaggtttt tgcttgtaaa aaagcagaaa accccattaa tgccaagcca
     8461 actatcctgt tccaattgca cacggaagac cccttattga gtaaacaggt atcatatcat
     8521 taaatgatga aaatgattat ctggttgcct tcaaattgat ccttgcaagt attgtgaact
     8581 ctggatcatt ttttagtaat tcaaatattc tgtataaatt taaaggcaat aaaaccgaat
     8641 gcaaaataaa tataggatgt aaatgataac ccaaaccgta agtaatgaac gcaacattac
     8701 tacaaacggc tatgagtctt aatgtcagaa tttttttgac ataaaatgtt gcaaagacta
     8761 aagaagaagc tacatatcca accaaatcaa tcattgattc cattttaacc tccacttcca
     8821 aatccctttc attcaacata acactgacca gaggagatta gcaagttttt taatcaattc
     8881 ttatggtttt cttaagcgtt gaaatgtata atcgatgatc tttgagttaa atatattacc
     8941 aaacaggaat tttttgttta tatttggaga caatatgccc cttacttcgt taaaaaaatt
     9001 tttaaaagat attggaaagg aaaatataaa acagcatcag gtaaaacttg tcttaaaaac
     9061 atctcaggat attcaattat ttttaaatgc tttgcgagat tcgagaaatc acggaatctc
     9121 gtccttatct gaacttgatc taagtgatac tcgctttact aatcaagagt tgagtgattt
     9181 agtcactgct cttaataata tacctggaat taaatcttta cgcttggatt cttgtggtct
     9241 taaggatagt gacacagtgg agctttccaa attaactcac atcaaaaaat tatcattgaa
     9301 aagtaattac ttaaagaacc gccctatgtt taattctatg cttgaagcat tgtatttgga
     9361 ttataataca gaacttagcg caagttatgt gttatttagt ttaagtcgca atgctgccgc
     9421 tttaaaaaaa ttatccttgc gtaactgtgg cgttactgat gccaatttag agtatttgac
     9481 tcggcctgag tccaggttaa aaagtctgac acactttaat ttgcgaagaa ataatatcac
     9541 tcaccaagga gtggatagct ttgctcatct tcaatcgttg actactattg atttaagtca
     9601 aaatactggg attggtgatg agggtgtatc caggcttgcg cctttaaagc agttaagaac
     9661 cctgtatttg gataattgtg gaataggggg agagggaatt aaggcaattg ccaaaatgaa
     9721 tttgcagact gttgatttga gtttcaatcc cgggttaaaa aaagaatggg gattggatga
     9781 cattcgaccg aatcatacca ttcgaacatt attgttaaca ttttgtagct taaatgataa
     9841 tcatgccaag cttattgttt cgaagtttcc agcggctacc gatttaaatg ttgcgaataa
     9901 taatatgacc agggcagggg taaagacttt attgagtaac ccgattatag aaaatttgga
     9961 tgtcagtacc caatcgcttt atgcaaaatc aacaggagct tctggcactg gtatgatctc
    10021 tccccgaata atgatgaaga aaaaggaaat aaaacaacag gaaaaagaaa aagtattgga
    10081 tttgctagat actatttgta ataccatcac attaaagagt attaatctgg aacatacagg
    10141 cataacctcc aggatgttat tgagtttaat tccagatgaa acagatcaca aacggtatct
    10201 taagaaaatt aatggtgttt cctgtaaaga gcttaagcca aaattagaac aacaaattgc
    10261 cttgagaaaa gctgaaaaac aaagttctcc tcctgtaatc attgctgtgg aagattccaa
    10321 accaagtgat ttaatgatta aatctggcag tagttccaag actgaggcgc aaaccaggga
    10381 agatcacttg atagccaatc ctgatgaaaa aataaaagct ctggaactag agaatcaaaa
    10441 attaaaggat gaactggcaa tagctcgtaa aagaattttt gaattggaaa atcagtcttt
    10501 atctggatcc aaaacaagaa aaaaaacctc aataccacca ttggcaatcc cggtgatgtt
    10561 tcaggatttt gcaaaaccag gaaaaaatga aacagatgaa aacaccagca actcaataac
    10621 ggcaatccct aaatagcaca gttattgtaa atgcattcaa catggctaac atccacttgg
    10681 atgttagtca tgtattgttt tactttttct tgccaaaaat gtgagttagg tgaccaatga
    10741 tggtttcaac ggcatttcgg catgccggtg tttcagagag atcattgagc atcaggaaat
    10801 catggattgt gcctagatga cgaactgcag ttactgccac acccgctgcg ttgagtttat
    10861 gggcataggc ttcgccctca tcacgtaaga catcacactc accggtaatg accaatgctg
    10921 gtggtaagcc tttaagttgt tcaagagagg cttgcagtgg acttgctgta atttcctttc
    10981 ttttttcttt attgggcaaa tacttatccc aaaaccactc catcgccttt ttggttagcc
    11041 agggaccttc agcaaattct ttataggaag cgttatcaaa attggcatca gttactggat
    11101 aaatgagtac ttgataatca attttggggc caccacgttc tttagcaaga agagtcatga
    11161 cgctggtcat attaccacca acactgtcac ctgcagcgac aaatcgtgaa gtatccagat
    11221 taaattgctt gccatgttct gctatatatt tcatggcagc gtaagattct tctatttgtg
    11281 tcgggtactg agcttctgga gctaaagtgt atttaacaaa cacgactgcg gtatgagtgc
    11341 ccactgctat ttctcgtact agacgaccat gagtttgata gtcgccaaaa acccagccag
    11401 caccatgaaa aaacataatg acaggtaagg cttcttttgc acctttaggg cgaacaatgc
    11461 gaatgtcaac cttaccgcca ggccctactg gcaaagtttt atcctcaaca tcgacatcag
    11521 gtttctcatt aggcaaagct tgtagttgat caaatatcgc cctaccttct gcaaccggta
    11581 tgtcataaat cggttttcca cccgcatttt gtattttttc aacaaatgac c
//
LOCUS       DATNSN010000107        11587 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.107, whole genome shotgun sequence.
ACCESSION   DATNSN010000107 DATNSN010000000
VERSION     DATNSN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.107"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..940
                     /gene="fabD"
                     /locus_tag="VAH76_002206"
     CDS             <1..940
                     /gene="fabD"
                     /locus_tag="VAH76_002206"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO1386812.1"
                     /translation="SAFVFPGQGSQSIGMLSDFMPQYTVVTDAFAEASDALGYDLWDL
                     VQNGPETKLNQTEYTQAAMLTADVAIYRLLMQLGVPQPQVMAGHSLGEYAALVCANSL
                     SLKDAAQLVARRGQVMQNAIPLGEGAMAAIVGLSDEQVRSLCEQASTANHLVTPANYN
                     ALGQTVVAGHRVAVDKLIQLAEEAGARLAMIIPVSVPCHCPLLKNAAELFAENLAQIE
                     FQVPSVDVISNVDLSIYHSTQHIRDKLKEQLYSPVRWVETVQLIQQRGIELVVECGPG
                     KVLNGLIKRIERNLTTISVYDTISLDQVIERLHIPV"
     gene            952..1698
                     /gene="fabG"
                     /locus_tag="VAH76_002207"
     CDS             952..1698
                     /gene="fabG"
                     /locus_tag="VAH76_002207"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO1386813.1"
                     /translation="MINLEGKIALVTGASRGIGRAVAEKLARNGAFVFGTATTEQGAA
                     SINSFFEKEKLAGKGIVLDVTSTEQVENLMSTLSDNDQCPSILVNNAGITADNLLLRM
                     DDEEWYKVIETNLNSIYRMTKACLKPMFRARWGRIISIGSVVGASGNSGQVNYTAAKA
                     GVIGFSKSLAQEIGSRGITVNVVAPGFIDTDMTTALPDMVKEEMLKRIPMRKLGQPED
                     VADAVAFLASDSAKYITGETIHVNGGMYMS"
     gene            1783..2031
                     /gene="acpP"
                     /locus_tag="VAH76_002208"
     CDS             1783..2031
                     /gene="acpP"
                     /locus_tag="VAH76_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1386814.1"
                     /translation="MSTVEERVRKIVVEQLGVKEEELKNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEKAEKITTIQEAIDYIESNLNKEEA"
     gene            2051..3289
                     /gene="fabF"
                     /locus_tag="VAH76_002209"
     CDS             2051..3289
                     /gene="fabF"
                     /locus_tag="VAH76_002209"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO1386815.1"
                     /translation="MNSRRVVITGMGMLTPVGLNVEETWRSILAGVSGVGLVEDFDTT
                     EYPTKIWAKVKNFNVENHMPIKDARKMDVFTQYGMAAADEAMLDSGLKIDEILSRRIG
                     VAVGAGIGGIQTITNNQDKLVAGGPRKVSPFFIPAGIINMVAGQISIKHNLKGPNISV
                     VTACTTGTHNIGLAGRIIAYGDADVMICGGAEMTTTPLCLAGFSAVRSLSKRNDEPEK
                     ASRPWDKDRDGFVMGEGAGILVLEEYEHAKARGAKIYAELVGFGMSGDAYHITAPDED
                     ADGASRAMEAAIQDAGIDPRQVDYINAHGTSTYLNDLNETKAIKRVFQNHAYDLAVSS
                     TKSMTGHLLGAAGAVEAIISILAIRDQVAPPTINLDNPDEGCDLNYVAHVPQSRTINY
                     VLSNSLGFGGTNGSLLFKRI"
     gene            3290..4288
                     /gene="mltG"
                     /locus_tag="VAH76_002210"
     CDS             3290..4288
                     /gene="mltG"
                     /locus_tag="VAH76_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO1386816.1"
                     /translation="MQFPRHKKLAILVLSLFFMSFLFLFWNIYRLLYTPIISKNASPM
                     IIALDKATSAYQFAYILQDKKLIHSAKFFLLIIRFEGLSHQLKAGVYQITPGETAMNL
                     LHRVVAGDVITQNFTIIEGSTQQKVDYDLRQANYLKYNPEDWAIVKENYPSAEGLLLA
                     DTYQYQGGSSSRALLEQAHRNLLSYLNTSWTNRAPNLPYKTAYELLIAASIIEKETAI
                     AQEKKLISGVMVNRLKKKMPLQMDPTVIYGLGNQYTGKLTHNDLLIQSPYNSYLNRGL
                     PPTPIAMVGKEAIDAAAHPQLSNYLYFVAKGDGTHQFSETYEQQRQAINQYRRKDY"
     gene            4288..4926
                     /locus_tag="VAH76_002211"
     CDS             4288..4926
                     /locus_tag="VAH76_002211"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO1386817.1"
                     /translation="MSSLTGKLIVIEGLEGAGKSTAVNLVVELLSQKKISTITTREPG
                     GTRIGEILRSIIKNPEYNNVLDDRSELLLLYAARIQLIEQVIKPALNEGQWVIADRFE
                     LSTLAYQGGGRKMDMRVINELSNFCLKGFKPDLTLYLDINPELGMIRAKSRGKFDRIE
                     QESIEFFHRIHDTYHILVKQNPEIMMIDANRPLEDVQSSIQSVIEEFIEHNL"
     gene            4923..5828
                     /locus_tag="VAH76_002212"
     CDS             4923..5828
                     /locus_tag="VAH76_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO1386818.1"
                     /translation="MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKL
                     LLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQR
                     TNHKFIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQF
                     AVFDELPCNNLLALGEYYTRELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQH
                     ELSAFLWFLYLLYSQLQYMHLFRVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLR
                     KLSHNMNMNHTLVLEDLLFSLVVDY"
     gene            5955..6296
                     /locus_tag="VAH76_002213"
     CDS             5955..6296
                     /locus_tag="VAH76_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEO1386819.1"
                     /translation="MTEKPQLINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQS
                     LELSIKLLNESEPYLVTGKIVWITPKGAQGNKPPGVGIQFTGENSRYLCNKIETYLAG
                     MLKSCQVTDTI"
     gene            6352..7140
                     /locus_tag="VAH76_002214"
     CDS             6352..7140
                     /locus_tag="VAH76_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO1386820.1"
                     /translation="MLVDSHCHLNFIDLTHFNHDLAQVINQARENGVEHFLSVCVELS
                     DYPQLERLAKDYPDISISVGVHPNSEMNYPVTAQMLCELAQNPACIAIGETGLDYYRN
                     PTEEAQSLQRDRFREHIKAALITSKPLIIHTRQAAEDTLLMMAKEGASQIGGVMHCFA
                     EDLDIAQRAIDLNFYISFSGIVTFKNAVSLQDVARQIPLEKILIETDSPYLAPVPHRG
                     KQNHPALVKHVAETIAQLRGMDFQEIAEITTNNFYNCFNIKRQG"
     gene            7252..8352
                     /locus_tag="VAH76_002215"
     CDS             7252..8352
                     /locus_tag="VAH76_002215"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEO1386821.1"
                     /translation="MSLYIGLMSGTSMDGIDAALLELPSNQLIHGITKQYSDDVRRNL
                     DDLIMGNHLTLASICQLNTLIGREFAEAVRQLLIEIKVHPKEIQAIGSHGQTVCHDTS
                     GNIPYTLQLGCGHTISSLTGITVVADFRTRDLVNGGQGAPFAPLYHQQIFSKVNESVA
                     VVNIGGIANVTFIAKNQMTRGWDIGPGNCLMDAWIYKNKGAPFDKSGVWASQGEVIHP
                     LLEYLLQDPFFHLDSPKSIGKEYFSLSWLQKHLKPDYTPADIQATLLALTAHTIAETI
                     LNESEEIKQLYLCGGGAHNTHLKENLARLLPGITVKSIAELGISPDYLEAMMFAWLAA
                     QTINQIPVNLTSITGAKGIAILGAVYPIIKSY"
     gene            8408..9700
                     /locus_tag="VAH76_002216"
     CDS             8408..9700
                     /locus_tag="VAH76_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386822.1"
                     /translation="MTAHYNVASNSGARNTLASSYFIFFLAASFYLYEFILQVAPSVM
                     AESMMKTFGVTGQGFGVISAFYFYAYAPTQLPAGVLYDRYGPRKLMTFAIVLCAFGSA
                     FFASTDSVFTACIGRFLIGIGSAFSFIGVLVLISRWFPPYYFAILAGVAQLMSSVGAM
                     FGEMPLAALIDLVGWRSASFILAGVGFLLAILFWVFIRDYPHQQNQTIPDHYLRDEWK
                     RLVAVCKHAYTWIIGGYAFAIWTPIAVFAALWGVPFLQEKFQVSVVAASGLCSMIWLG
                     IGVGSPLLGWLSDKLESRRIALIISAVLGLIATLIILYWSELSYNWSYVVLFVLGLGA
                     GGQTVSFAVVKENNTPELVGTASGFNNLSVLIGGAIFQPLVGYILQQTGSWRIVNGAH
                     VYSISSYQTALLVMPVCFLASLLIATFLLKESHPARRG"
     gene            9773..9916
                     /locus_tag="VAH76_002217"
     CDS             9773..9916
                     /locus_tag="VAH76_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386823.1"
                     /translation="MGKDYIGASRYPESVQAEEMRKHRLEALARDIKFHTRLRDGINA
                     SSA"
     gene            complement(10219..11499)
                     /locus_tag="VAH76_002218"
     CDS             complement(10219..11499)
                     /locus_tag="VAH76_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386824.1"
                     /translation="MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKS
                     FNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHS
                     MNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
                     IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNY
                     KTLLSNQAFCYSAIAEGLVGIPCIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATIL
                     GNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLS
                     VINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNA
                     VGLLFLIFIGLTFFIGTPKKSEIHDEANSNVANS"
BASE COUNT         3531 a         1968 c         2500 g         3588 t
ORIGIN      
        1 atcagcattt gtatttcctg gacagggatc gcagtctata ggcatgttgt ctgactttat
       61 gccacagtat actgttgtca ccgatgcttt tgcagaggcc tcagatgcct tgggctatga
      121 tttgtgggat ttggttcaaa atgggccaga gacaaagcta aaccagactg aatacacgca
      181 ggctgccatg ttaactgctg atgttgcaat ttatcgttta ttaatgcaat taggcgtacc
      241 ccagcctcaa gtaatggctg gccatagttt gggggaatac gctgctttgg tttgcgctaa
      301 ttctttatct ttaaaggatg ctgcacagtt agttgctcgt agagggcagg tgatgcaaaa
      361 cgcgatccct ctgggagagg gggctatggc tgccatagta ggcttatctg atgaacaggt
      421 tcgttcattg tgtgagcagg ccagcacagc caatcatctt gttaccccgg caaattataa
      481 tgccctgggc caaactgttg ttgctggaca tcgagtcgcc gttgataaac taatacaatt
      541 ggctgaagag gctggcgcca gactggcaat gataattcct gtaagtgtgc cttgtcattg
      601 ccctttatta aaaaatgctg cggaattatt tgctgaaaat cttgctcaaa ttgaatttca
      661 agttccttca gtcgatgtaa taagcaatgt tgatttaagt atttatcatt ccacccaaca
      721 tataagagat aagctcaaag agcaactata cagccctgtg cgttgggttg aaacggttca
      781 gttaattcaa cagcgcggaa ttgaactggt tgtggagtgt gggccaggaa aagttctgaa
      841 tggtttgata aaaagaattg agagaaattt aactacgatt agcgtttatg acaccattag
      901 tcttgatcaa gtcatagagc gtttacatat cccagtttga gaggaaacta gatgatcaat
      961 ttagaaggta aaattgcatt agttactggt gccagccgtg gcataggtcg tgctgttgcc
     1021 gagaaattgg caagaaatgg tgcctttgtt tttggaacag caaccactga acagggtgct
     1081 gcgtctatta attctttttt tgagaaagaa aaattggctg gtaaaggaat cgttttggat
     1141 gtaaccagca ctgagcaagt agaaaatttg atgtctacct tgtctgataa tgatcagtgt
     1201 ccttctattc ttgttaataa tgctggcatt accgcagata accttttatt gcgtatggat
     1261 gatgaggaat ggtacaaagt aatagaaaca aatttgaatt ctatttacag aatgactaaa
     1321 gcttgcttaa aacctatgtt cagagcacgt tggggaagaa taatttctat aggctctgtg
     1381 gtaggtgcca gtggcaattc tggacaggta aactatactg cagccaaggc tggagttatt
     1441 ggtttttcca agtctttggc acaggaaatt ggtagtagag gaataactgt aaacgtggta
     1501 gcccccgggt ttatcgacac agatatgact actgcattgc ctgatatggt gaaagaggaa
     1561 atgttaaaaa gaattcccat gcgtaagctg ggccagccag aagatgttgc tgatgctgta
     1621 gcttttctag catcagacag cgcaaaatat atcacaggtg aaacaattca tgtcaatggc
     1681 ggtatgtata tgagttaaat gcacttgcgt tatctgtata attgaataaa ctagccgtca
     1741 gtatttaata atttctatca accgaaagag gaaaattacg ttatgagtac agttgaagag
     1801 cgtgttcgca aaattgttgt tgaacaactg ggtgttaaag aagaagaatt aaagaatgat
     1861 gcatcctttg ttgatgattt gggtgctgat tccctggata cagtagaatt ggttatggct
     1921 cttgaagaag aatttgaaac agaaattcct gatgaaaaag ctgagaaaat tactacaatt
     1981 caagaagcaa ttgattatat tgaatctaac ttaaataaag aagaagctta atttttttaa
     2041 ggagctctca ttgaatagtc ggcgtgtggt tattaccggc atgggtatgc ttactcctgt
     2101 cggacttaat gtagaagaaa cttggcgaag tattttagct ggggttagtg gggtaggttt
     2161 ggtagaagat tttgacacta ctgaataccc aactaagatt tgggctaagg tcaagaattt
     2221 taatgttgag aatcacatgc caattaaaga cgcccgcaaa atggacgtct ttacacaata
     2281 cggtatggct gctgccgatg aagcgatgct tgactcgggt ttaaaaattg atgaaatctt
     2341 atctcgtcga atcggtgtcg cagtaggagc aggtattggt ggtattcaaa caatcacaaa
     2401 taaccaggat aaactggttg ccggtggacc acgtaaggtt tcgccatttt ttattccggc
     2461 aggtattatc aatatggttg ctggacagat ttctattaaa cataatttaa aaggacctaa
     2521 tatctcggtt gtgactgcat gtactaccgg aacacataat atcgggctag cagggcgcat
     2581 aattgcttat ggcgatgccg atgtcatgat ttgtggtgga gcggaaatga ccacaacgcc
     2641 actttgtctt gctgggtttt cagcagtaag atctttgtcc aagcggaatg atgagcctga
     2701 gaaagcctca aggccttggg ataaagacag agacggtttt gttatgggtg agggtgctgg
     2761 tattttggtt ttagaagaat atgaacacgc taaagctcgt ggagccaaaa tttatgcaga
     2821 attggttggt tttggtatgt caggagatgc ttatcatatt actgcacccg atgaagatgc
     2881 cgatggagct tcacgagcta tggaggcagc catccaggat gctggtatcg atcccagaca
     2941 agttgattat attaatgctc acgggacgtc gacttattta aacgatttaa atgaaacaaa
     3001 agccattaag cgtgttttcc agaaccatgc ttatgatctg gcggttagct caaccaaatc
     3061 catgacagga catttgctcg gagctgcagg tgcagtagag gcaattataa gtattttagc
     3121 cataagagat caggtggctc cacctacaat aaatttggat aatcctgatg aaggttgtga
     3181 tttgaattat gtagcacatg taccacaaag cagaacaatt aattatgttc taagtaattc
     3241 acttggtttt ggtgggacaa acggtagctt actgttcaag cgaatctaaa tgcaattccc
     3301 tagacataaa aaactggcta ttcttgtttt atccttgttt tttatgtctt tcttgttttt
     3361 attttggaat atatatcgct tattgtatac accaataatt tcgaaaaatg cttctccaat
     3421 gattattgcc ttggataagg cgacgtccgc ttatcaattt gcttatattt tgcaagataa
     3481 aaaattaata cattctgcta aattttttct attaattatt cgttttgaag gattatcgca
     3541 tcagttaaag gcaggcgtgt atcaaattac tcctggcgaa actgcaatga atttactaca
     3601 tcgagtagtc gctggtgatg taattacgca aaattttacg atcatcgaag gatctacaca
     3661 gcagaaagta gattatgacc taagacaagc taattatttg aaatataatc cagaagattg
     3721 ggcaatagta aaagaaaatt acccgagtgc agaggggtta cttctagcag acacttatca
     3781 ataccaaggt gggagtagta gtagggcatt attagagcaa gcccatcgta atttattaag
     3841 ttatcttaat actagctgga caaacagagc accaaatttg ccttataaaa ctgcttatga
     3901 gctattgatt gctgcgtcaa ttattgaaaa agaaactgca attgcgcaag agaaaaagtt
     3961 gatttctggt gtgatggtaa acaggttaaa gaaaaaaatg ccattacaaa tggatcccac
     4021 agttatctat ggactgggaa atcaatatac aggaaaatta acacataatg atttgctaat
     4081 tcaatcacca tataattctt atttgaacag agggttgcct cccaccccaa tagctatggt
     4141 tggtaaagag gccatagatg ccgctgcaca tccgcaatta tctaattatt tatactttgt
     4201 tgccaaagga gatggcactc atcaattctc cgaaacttat gagcaacaaa gacaagcaat
     4261 taaccaatac agacgcaagg attattaatg tcatcgttaa ccgggaaatt gatagttatt
     4321 gaaggtttgg agggggcagg aaaatctaca gctgtcaatt tagttgttga actgttatcc
     4381 caaaaaaaaa tcagtactat aaccacgaga gaacccggag gaacaagaat aggtgaaatt
     4441 ttaagaagta ttattaaaaa tccagagtac aataatgttc tggatgacag aagcgaatta
     4501 cttctcctgt atgccgcaag aatacaactg attgagcaag tgatcaaacc cgctttaaat
     4561 gaggggcagt gggtcatagc tgatcgattt gagctttcca ctttagctta tcagggaggg
     4621 ggccgcaaaa tggatatgcg ggttattaat gagctatcga atttctgttt aaaaggattc
     4681 aaacctgatc ttactttata tttagacatt aatcccgagc ttggcatgat aagagctaaa
     4741 tcaaggggaa aatttgacag gattgagcag gaatcaattg agttttttca tagaatacat
     4801 gatacatatc atattcttgt taaacaaaac ccggaaatta tgatgattga tgctaacagg
     4861 cctttggaag atgttcaatc ttccattcaa tcagtaatcg aggagtttat agagcataac
     4921 ctatgattac ttatcaagaa caatggcaga aaatgcaaat ggccttggcg ataagacgaa
     4981 ttcctcaagc catgctattt ttgggttctt tgcattgtgg tcttatagaa tttacaaatc
     5041 aagtgatcaa attactcttg tgtaaaataa aacagaatga gccttgtttg acttgcatgg
     5101 attgccaaat gatagagcgt tttgagcatc cggatgtgga atggattaag cctgaaagaa
     5161 gcggtggtat tattaaaatt gatcaaatta ggggattgca gagtacggcg tttctaacac
     5221 cacagaggac taatcataaa tttataatta tcgaatcagc tgaatcaatg aatattgcgg
     5281 catcaaatgc cttattgaaa attctggaag agccagcgca acatactatc tttattttgt
     5341 tagcgcaaca aattggcaat ataccaccca ccgtattgag ccgatgccaa ataacgcaat
     5401 ttgctgtttt tgatgagttg ccttgcaaca atttattggc tttaggagag tactatactc
     5461 gggaattgaa tagcacaatg gtcttcaagc attcagaatc tattttagaa ggcttgattg
     5521 caattattga gcgaagagag catccatgca ctttggctgc acaatggatt caacatgaat
     5581 taagtgcttt tttgtggttt ttatatttgc tctattctca actacagtat atgcatttgt
     5641 ttcgagtggc ttattctggt acgttaacaa aacaattgaa taagctatcc tcattactca
     5701 atccggttat gatttttgcc caagtagata aaatcaatac tttgttaaga aaattaagcc
     5761 ataatatgaa tatgaatcat acattggtct tggaagattt attattttct ttggttgttg
     5821 attattaatt gagtttaaag gaattaaacc attgcgtgtg aaccgagttt tttattaagc
     5881 aactgaagtg agcaagatga ttagttaaaa caggactatt caaggaggat gtctattgat
     5941 tgaacggagg agttatgacc gaaaaaccac agcttataaa ctgctcattt cccaatgaag
     6001 catctttgta catggcttac atgccttttg tgaaaggtgg cggtcttttt ataagaactc
     6061 aacacgttta ttctctaggg cagtcgcttg aattatctat taaattgctc aatgaaagtg
     6121 aaccatattt ggtcactggt aaaatagtct ggattactcc taaaggtgca caaggtaata
     6181 agccgccggg agttggcatt caatttaccg gtgaaaacag tcgttatcta tgtaacaaga
     6241 tagaaactta tttggcagga atgctaaaat catgtcaggt tacagatact atatagaatt
     6301 ttcatttatt catgaacaat atattcattg atattatttt ggggtataaa ggtgctggtc
     6361 gactctcatt gtcatttaaa ttttatcgat ttgactcatt ttaatcatga tcttgctcag
     6421 gtaatcaatc aggctcgcga gaatggggta gagcatttct tgtctgtttg tgttgagttg
     6481 agcgattatc ctcaattaga gcgtttagct aaggattatc ctgatattag catttctgtt
     6541 ggagtgcatc cgaatagtga aatgaattat cctgttacgg cacagatgtt atgtgaattg
     6601 gctcaaaatc ctgcctgtat tgccatagga gaaacagggc ttgattatta tcgtaatccc
     6661 actgaggaag ctcaatcttt gcaacgagat cgcttcaggg aacatatcaa ggcggcgctt
     6721 ataacttcca agccgttaat tattcatacc aggcaagctg ctgaagatac tttgttgatg
     6781 atggctaagg aaggagctag tcagataggc ggggtcatgc actgctttgc tgaagacttg
     6841 gatattgcgc aaagagcaat tgatttaaat ttttatatat cattttccgg gatagttact
     6901 tttaaaaatg ctgtttcatt gcaagatgta gccaggcaga tacctttaga gaagatattg
     6961 atagagacag attcacctta tttggctccg gttccacaca gaggaaagca aaatcatccc
     7021 gctttggtga aacacgtagc agaaactatc gcacaactca gagggatgga tttccaagag
     7081 atagcagaaa tcactacaaa caatttttat aattgtttta atatcaaacg acaaggataa
     7141 atcattggta aatcgtagtt ctcaacttca aagcagaagc agtaattttt ttgcctgtga
     7201 ataaaggttt tagtagtatt gatatatcct attgcaaatt tagagtaatt aatgagttta
     7261 tatatcggtt tgatgtcagg taccagtatg gatggcattg atgccgcctt gctggagctt
     7321 ccttccaatc aattgattca cgggattact aaacaataca gtgatgatgt caggagaaat
     7381 cttgatgatt taattatggg taatcattta accctggctt cgatttgcca actgaacacg
     7441 ctaattggca gggagtttgc agaagcagtt cgccaactct tgattgaaat aaaagtgcat
     7501 ccaaaagaaa ttcaggccat tggtagtcat ggtcaaactg tctgtcatga tacaagtgga
     7561 aatatcccgt atactttgca gcttggctgt ggccatacga tttcttcttt aactggcatt
     7621 accgttgtag cagattttag aacaagagat ttggtgaatg gtggtcaagg tgcgcctttt
     7681 gctccccttt accatcaaca gatattttct aaagtgaatg agtctgttgc tgttgttaac
     7741 attggtggta ttgctaatgt tacatttatt gcaaagaatc aaatgactag aggttgggat
     7801 atagggcctg gcaattgtct gatggatgcc tggatttata aaaataaagg agcccctttt
     7861 gataaaagtg gggtttgggc gagccaaggt gaagtgattc atcctctact tgaatatttg
     7921 ttacaagatc catttttcca tttagattca cctaaaagca taggcaaaga atatttttct
     7981 ttatcctggc tacaaaaaca tttaaaaccg gattacacac cagctgatat tcaggctact
     8041 ttgttggcgc ttacagccca tacgatagct gaaacaatat tgaatgaatc agaagaaatt
     8101 aaacaattat atctctgtgg aggcggggcc cataatacgc atttgaaaga gaatttagct
     8161 cgtctactgc ctggaataac agtaaaaagc atagcagagt tagggattag tcctgattat
     8221 cttgaggcaa tgatgtttgc atggttagca gcacagacga ttaatcaaat accagttaat
     8281 ttaacatcaa taactggagc taaaggtatt gcaattttag gtgcagtgta tcctataata
     8341 aaatcatatt gacaaactaa agaatcataa ggtttaattt cataaaaatt aacagagtga
     8401 ttttaaattg accgcacatt acaacgtcgc ttcaaacagt ggagcaagaa atacacttgc
     8461 atcgtcttat tttatttttt ttctagccgc ttcattttac ttgtatgagt tcatactaca
     8521 agtagcacca agcgttatgg cagagtcgat gatgaaaact tttggggtga cgggacaagg
     8581 ctttggtgtc atttcagctt tttattttta tgcttatgcc cctacacaat tgccagcagg
     8641 agtattatac gatagatatg gaccacgtaa attaatgact tttgcaatcg ttttgtgtgc
     8701 atttggttct gccttttttg cttccactga cagtgtattt acagcttgca taggccggtt
     8761 tttgatcgga ataggctctg cgttttcttt tatcggtgtt ttggtcctta tttcacgctg
     8821 gtttccccct tattattttg caattctagc aggggtagcg cagttgatga gttcagtcgg
     8881 cgctatgttt ggtgaaatgc cgttggcagc cttaattgat ctggttggat ggagaagtgc
     8941 gagttttatt ttagcgggtg ttggatttct cttggctatt ttattttggg tatttattcg
     9001 agattatcct catcagcaaa atcagacaat ccctgatcat tatttaagag atgaatggaa
     9061 gcgcttagtt gctgtctgta aacatgctta tacatggatt atcggtggtt atgcttttgc
     9121 aatttggact cctattgcag tatttgctgc actttggggg gttccttttt tacaggaaaa
     9181 atttcaagta agcgtagtgg ctgcctcagg cttatgcagt atgatttggc taggcattgg
     9241 tgttggtagt cccttgttag gatggttgag tgataaattg gagagtcgcc gtattgcatt
     9301 gattatcagt gcggttttag gactgattgc aactctgatt atcctttatt ggtctgagtt
     9361 atcttataat tggtcttatg ttgtcttgtt cgttttaggc ttaggtgctg gaggacagac
     9421 ggtaagcttt gcagtagtta aagaaaataa tacaccggaa ttagttggca cagcatctgg
     9481 ttttaataat ttgtctgtat taattggtgg ggccattttc cagcccctcg ttggttatat
     9541 cttgcaacag acaggatcat ggcgaattgt taatggtgct catgtatata gcatatcgag
     9601 ttatcagact gcgttgcttg ttatgccagt atgcttcctg gccagtttgc ttattgccac
     9661 ctttttgctt aaggaatcgc atcctgcgcg cagaggttaa taacagattt atcaattcaa
     9721 tattctagcc ccgatatctt tttgattagg cttaaataat ataacgtgag ttatgggtaa
     9781 agattatata ggagccagtc gatatccgga atccgttcag gctgaggaga tgcgtaagca
     9841 tcgtctcgaa gccttggcac gagacattaa attccataca aggcttcgtg acggcataaa
     9901 tgcctcctca gcctgaacgg cttgagataa cggctgatct tactcataac ttacgttaat
     9961 ataattggag tcttggtagt gtgcggataa agcgagctaa gaaaatattc agctaaaatt
    10021 tgctgtaatc tgttggtaat tgagagatct aaattcttat attagacttt ttgaacaagc
    10081 tgcctcttta gcctaggtgt tgcgttgtaa aagttttaat atagcaacaa tttgatgcat
    10141 gttaactcca ttcacctgtt catgtttccg ttattgacct aaaacaaagg aatcaggtta
    10201 tgtaaatgtg gcctgttctt atgagttagc cacattacta tttgcttcat catgaatttc
    10261 agatttcttg ggagtcccta taaaaaaagt taaaccgata aagattagaa ataataatcc
    10321 aacggcatta caatagaggg cgaaatgcag attattgtga tgttgataaa acaggttagc
    10381 tatttctatt ccaatagccc caatgatcat aatacttagg ctgattaatg ccgaggctgt
    10441 tcctttgctt acagcggtta caaataaaca atatctgttc aagggggcat taattacact
    10501 cagtgagaaa aagtaaatta tgattcccgg gatcaaatac acataattat tgccataaaa
    10561 ataagggaga agagctgtca gagcaagtcc cacgaccatt atgatacaac ctataaaaat
    10621 aattctctcg attttatatt tgtaagttaa atgatgtaag caccagtttc ctaaaattgt
    10681 tgccccgaag atgggtagtt gccaaaggcc atattgaatt acagttaatt ttgcttcagc
    10741 aattaatatg attggcgcaa gcgcaatcca ggcgatgcat gggatcccta ccagaccttc
    10801 agcaattgca ctataacaaa aggcctgatt agataataac gttttataat ttcgggttat
    10861 agcgttcagt gcgaacggtg ttttaggtat aagctggccg tctcttttta cctggcctat
    10921 gggttctggc ataaaccgcc ataatcccca ataagctaaa agagcaccca aagcaataat
    10981 aataaaaatc aaacgccagg acgcataatg aatgattatg gctcctaata gtggccctaa
    11041 taatggggct aatattgcgg cattagccat gatagcaatt aatcggatag cgtccatttc
    11101 ttcaaatatt tcttgaattg tggcataacc tatcacacca atgaaacaca atcccattcc
    11161 ctgaaaaaac cgggcgatta aaaattgatt catggagtgt gagcttgcaa tcaaaagagt
    11221 gaacagaaaa aataagcatg atcctattaa catcattggt cttctgccat aagcatctga
    11281 tagtgggcca agtatgagct gcaaactggc ccctccgagg acatagacag ttagagaggt
    11341 ggcaacaaca ctttcatgcg cgttgaatga tttaacaaca ttgatcatac ctggcatgat
    11401 catgtcattg gctatataag tcaaaaattc atataaaacc aggaagcaag caaagataat
    11461 ggcctgtctg tatgaaattt taattagtgg ctcagacata gcattaaccc tgaatcaatc
    11521 gtttctaaaa ggcggtctat cagtatatac gtttatttca taatttggta gaaaatctga
    11581 taaatgt
//
LOCUS       DATNSN010000108        11106 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.108, whole genome shotgun sequence.
ACCESSION   DATNSN010000108 DATNSN010000000
VERSION     DATNSN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.108"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(386..745)
                     /locus_tag="VAH76_002219"
     CDS             complement(386..745)
                     /locus_tag="VAH76_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2149 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386825.1"
                     /translation="MMSREKAAGTFFKDYQKKNVMRLLQDSLEKIIKEWLKTDDESHT
                     KLKSLQELSEMDINATSFAEHSPLPDFVMRLWQDPHKALDAMDKNISKNEIRKLIKET
                     AKEIELVFTHQKAPGYS"
     gene            complement(833..2113)
                     /locus_tag="VAH76_002220"
     CDS             complement(833..2113)
                     /locus_tag="VAH76_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2148 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386826.1"
                     /translation="MIVLGINMTKIKLESPGFMVHKKLKSMFQSYGVMMTGVPAEVLG
                     QMQAERSIPSINKTGNLKQQIAKEVSKVCHMMTEPTQSCGQASNDVCELLLGKIEAEK
                     FHFTKYEALSTDGDNLKNVLENTDPSSTNLLIRFEIDREDPPIVLVKTKNENFNPETA
                     VRNKIYLLENKLYFLDKMGNLFNLGPDKKKCTRLFNAIGDSAEYSLCDPFVLEEPEKP
                     EDFAISEIVDIFNEQKERFDFWIGSHSFTIYIPQTLGESPRQFYPYQAYFGSHTLQDW
                     FVSGKDEYLSRIGIDKYIEKLALLGKTTNTKERSDIYAEFFSKRGREAFFCAHLNEKR
                     QPLRVKFKITEINPELALKNLQETQEFIDTHPGENPSDKVENYRNRAKLAMTEHLESL
                     LDIKPELSPSLFWKNVDKKEQEMTDSSRYKKGPC"
     gene            complement(2189..3637)
                     /gene="mavC"
                     /locus_tag="VAH76_002221"
     CDS             complement(2189..3637)
                     /gene="mavC"
                     /locus_tag="VAH76_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavC"
                     /protein_id="HEO1386827.1"
                     /translation="MTTSKLEKTGLHVHEKIKHMVKSYGTMITGIPAEILGQNEAEIS
                     VGYVKKMGNMKENIAEVVRKSEMTQPTNSCGKASNEVCDLLLGTEGASEFEKSNYQVL
                     SGDGINLKGSLPNKNLLVRVEMDRFNAPQKYQKIKREEFNPETAEKNKIYLLEDQLVY
                     LDIFGKVIDLGQTSDTCHRLFNAITTPFYQNYILYDEYIDPKESAEEAAMFEMGEIVT
                     AKMKNIDCWTATHSFTIFVPEAESEETRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLG
                     IDGYIEKLALLGTTTDSKIRSSIYGELFSPPGKEHVFCTGINEKFSPLRVKFKVTEVN
                     PEIALQNLQEVQEFIDTNYPGENPKAQCELYKTKAQEAMTKQLEMRLLTEPTHRKESV
                     PPYKEKNTQSFFSKNLDTTSRIDKMISSVITMENLKILAKEADTLSGNDREKLVEYFK
                     TLPSEQLAEIKPESETAQKAWDEQYGLVLRGK"
     gene            4051..6501
                     /locus_tag="VAH76_002222"
                     /pseudo
     CDS             4051..6501
                     /locus_tag="VAH76_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            6491..6910
                     /locus_tag="VAH76_002223"
     CDS             6491..6910
                     /locus_tag="VAH76_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386828.1"
                     /translation="MRAKNDECTHVLLVDDDEIDLMSVQRALTKLNIPVCFHIARDGV
                     EALEQLSGNIKATKPKIIVLDIMMPKMNGIEFLRELRSHIHYNPIKVVIFTTSSNIRD
                     KQATTGLDIAGYFVKDSQFNEFIYCCKHLLEKKSHLS"
     gene            complement(6941..7459)
                     /locus_tag="VAH76_002224"
     CDS             complement(6941..7459)
                     /locus_tag="VAH76_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector E3 ubiquitin ligase
                     AnkB/LegAU13/Ceg27"
                     /protein_id="HEO1386829.1"
                     /translation="MKKNFFSDLPEETIVNTLSFLKANTLGRIAQTCQFFNRLANDKH
                     LELHQLRQQRIKRELWGNLMMAARSNNLEEVKKILKKGIDPAQTNSYHLNRTPLLAAI
                     EGKAYQTANYLWRKYTFDPNFKDNYGDSPISLLKKQLANPAFKDKEKKQIRALIRGMQ
                     EEKIAQSKCLVC"
     gene            7692..8633
                     /locus_tag="VAH76_002225"
     CDS             7692..8633
                     /locus_tag="VAH76_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEO1386830.1"
                     /translation="MILNITAAQFPDLTLNAIESCQNIYRSIDFNFGEDADTAINKAS
                     LEKFINQFKSIHSTHDKPIEGIITVGKMKNVSPDTVKLLLTTEDFVQMLDQKSFLKLI
                     VTSNEIANFVLDNPKLRAKLDGIEPLVDAQKFENSCTARAIMKILLERGLIEPSSYTP
                     GKELEIYKDIWLEPGKVASPEKIASYFCKYNLNVIGVEIRELSKSVRNKYSKDMVITS
                     LYSLFKKEVPIRKKMTLTTLSEADFPEGITTLIIIKAGVLHTLLGKKHHGQFEVTDPW
                     FGDKKIYSGFMEFLEKERKNLGVFFEISQGPQEIFRP"
     gene            8795..8953
                     /locus_tag="VAH76_002226"
     CDS             8795..8953
                     /locus_tag="VAH76_002226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386831.1"
                     /translation="MPSRSHVWNLMFRAKASRRCYASPQPKRTLDIDCFLYNPYPEPT
                     SDKSETRH"
     gene            complement(9010..9774)
                     /locus_tag="VAH76_002227"
     CDS             complement(9010..9774)
                     /locus_tag="VAH76_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO1386832.1"
                     /translation="MFNQNLAIIIGGSSGMGLETAKKLSSNNMELLITGKDKQKLENA
                     AAEIQKLGAKVDILVLDLYDKEQVFQFMSSMSDETRRVKYLVNAAGYFKPITFLEHEE
                     EDYDRQVNLNKAFFFITQAVVKNMKKNGGGSIVNIGSMWANQAVKATPSSAYSMQKAG
                     LHSLTQHLAMELAEHNIRVNAVAPAVVATPIYKSFIDEERIEESLQDFNKFHPIGRIG
                     KATDIADAIEFLLSEKASWITGEIMNVDGGVMAGRN"
     gene            9869..10249
                     /locus_tag="VAH76_002228"
     CDS             9869..10249
                     /locus_tag="VAH76_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1386833.1"
                     /translation="MVQIIEMDEKKIIQKKAPIKTASMVEAIYGCKWSLTIYSLLRNG
                     INRPGEMVRSVEGLSTKVLNQCLRKNIEFGILGRNTFHEIPPRVEYFVTPFGEKFLKI
                     LDQIEELNEQIRDYQQIEFHNNDS"
     gene            10383..10970
                     /locus_tag="VAH76_002229"
     CDS             10383..10970
                     /locus_tag="VAH76_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO1386834.1"
                     /translation="MARNDPIALLTTWISEELERGAPNPQHATLSTAGKDSIPHGRVI
                     AIREINEQGLLFFTQRGTRKVVEMTYNPIVSLTFWFELFEREVMIDGSARPLSESENQ
                     AYWDTYPPFAQIRFHSYAPTSSQVISSKQLIENKRKILISRFHDKSLPMSEYYCGFRI
                     IPTRMVFYTYRTDELSDVVEYCFIKNNWEKNLLSP"
BASE COUNT         3380 a         1998 c         2076 g         3652 t
ORIGIN      
        1 gaagtgcctg tgatatactc tgcaatcgcg ggattaattt ttgatttttc caataattct
       61 ccataagact tgacgctgtc aaatctcgta ggggtgtttt ttataaaatc attttttaac
      121 tctttaatta aatccaaaat atagcatggc attagctttt aaccccttat atttaaatta
      181 atatgtagtg gaataaaaac gaatctcagg agactcgatt ccatagaatt aatttaacat
      241 gataatgaat tagaaggggt tttggatgtg catcaatttt cgaattgttt ttctatgaat
      301 gtttttatcc aataagacat catttggcca atgatatggc caaaatgaat gaagaattaa
      361 tataccagca ccatcctcat ttcaattaag agtagccggg tgctttttga tgagtaaaga
      421 caagttctat ttccttcgcc gtttctttaa tcagctttct gatttcgttt tttgaaatgt
      481 ttttgtccat ggcatcaagc gctttatgtg gatcctgcca gagacgcata acaaaatcag
      541 gaagtggtga gtgttccgca aatgaggtag cattaatatc catctctgaa agttcttgaa
      601 gacttttcag tttggtatgg ctttcatcat cagttttcag ccattctttg ataatttttt
      661 cgagagagtc ttgtaacaat ctcattacat tttttttctg gtaatcttta aaaaaagttc
      721 cagctgcttt ttcgcgactc atcataaatc aaactccttc gttattttca aacacccatt
      781 aatacaattt tagaacaatt tataatttaa ttgattttta ttgtttcatt gactaacatg
      841 gtcccttttt atacctgcta ctgtccgtca tttcttgttc tttcttatcg acatttttcc
      901 aaaataaaga ggggctcaat tcaggcttga tgtctaatag gctttccagg tgttcggtca
      961 ttgccaattt cgctctattt ctataatttt ctactttatc tgatggattt tctcctggat
     1021 gggtatcaat aaattcttgt gtttcctgta aattctttaa tgccaattca gggttaattt
     1081 cagttatttt gaactttact cgtaatggtt gtcttttttc attaagatgg gcgcaaaaaa
     1141 atgcttcccg gcctcgcttt gaaaaaaatt ctgcataaat atccgagcgt tctttcgtgt
     1201 ttgttgtttt tcctagtaaa gctaattttt caatatactt gtcgatgcca attcgcgaca
     1261 gatattcatc tttaccgctg acaaaccaat cctgtaaagt gtgtgaacca aagtaagctt
     1321 gataaggata gaattggcgt ggtgattcac ccagtgtttg agggatatat attgtaaaag
     1381 aatgcgaccc gatccaaaaa tcgaatctct ctttttgttc gttaaagata tcgacgatct
     1441 cacttattgc aaaatcttca ggtttttcag gctcttcaag aacaaaagga tcacaaagac
     1501 tatattctgc tgagtctcca attgcgttga ataaccgcgt acattttttt ttatctggcc
     1561 caaggttaaa aagattaccc atcttgtcaa gaaaatataa tttattttct aataggtaaa
     1621 ttttattcct cacagcggtt tccggattga aattctcgtt ttttgttttg accaatacaa
     1681 tgggagggtc ttctctgtca atttcaaatc gtattaatag gttagtgctg gatggatctg
     1741 tattttccaa aacatttttt agattgtctc catctgttga taatgcttca tatttggtga
     1801 aatggaattt ttccgcttca atttttccta aaagcaattc acacacatca ttactggctt
     1861 ggccacaact ctgagtgggt tcagtcatca tatgacatac ctttgaaacc tcctttgcaa
     1921 tttgttgctt aaggttccct gttttattaa tgctggggat agatcgctct gcctgcattt
     1981 gtcctaacac ctcagcaggg acaccagtca tcatgactcc atagctttgg aacatgcttt
     2041 tcaacttttt gtgtaccatg aaccccggcg attccagttt tatttttgtc atattaatgc
     2101 caagtacaat caaagtgata tattatagat caatatcatt tgattataaa cagttcaagt
     2161 gacaaataca caaagccgat tcgatttatc acttaccacg aagaactaat ccgtattgtt
     2221 catcccaggc tttttgagct gtttctgatt caggcttgat ttctgccagc tgctctgatg
     2281 gtaatgtttt aaaatactcc actaactttt cacgatcatt tccagagagg gtatctgctt
     2341 cttttgcaag aatcttgagg ttttccatcg taatgactga tgagatcatt ttatctattc
     2401 tgctggttgt atctaagttt ttgctaaaga aagattgtgt gtttttttct ttataggggg
     2461 gtactgattc ttttcgatga gtcggctctg ttagaagtcg catctcaagt tgtttagtca
     2521 tcgcttcttg cgcttttgtt ttataaagtt cgcattgagc tttcgggttt tcgccaggat
     2581 aattcgtatc aataaactct tgaacttctt gtaaattttg caatgctatt tctggattga
     2641 cttcagtgac tttaaatttg actcgtaatg gcgagaattt ttcgtttatc ccggtacaaa
     2701 aaacatgttc ttttcctgga ggagaaaata gttctccata aatgctggag cgtattttgc
     2761 tatcggttgt tgtgcctaac aaagccagtt tttcgatata gccatcaata cccaagcgtg
     2821 ataatttttc atctttatca ccggaaaacc actgttgcaa agtatggctg gtccaataag
     2881 cttgataagg atacaatgtg cgtgtctcct cactctctgc ttcaggaaca aatatagtaa
     2941 aagaatgggt tgctgtccaa caatctatgt ttttcatttt agctgtaact atctcaccca
     3001 tttcaaacat ggctgcttcc tcagcacttt ctttcggatc aatatactca tcataaagaa
     3061 tataattttg ataaaaaggg gtggtaatag cattaaatag tctatgacat gtgtctgatg
     3121 tctgtccaag gtcgatgact tttccaaata tatcgagata tacaagctga tcttccagta
     3181 gataaatttt atttttttcc gcggtctcgg gattaaactc ctctcttttt attttttggt
     3241 atttttgagg agcattaaat cgatccattt caaccctgac cagtaaattt ttattgggta
     3301 gagaaccttt caaattaatg ccatccccag ataagacttg atagtttgat ttttcaaatt
     3361 cgctcgctcc ttctgttcct aaaagtaaat cacacacttc attgctcgct tttccgcagc
     3421 tgtttgtggg ttgagtcatt tcagattttc gaactacttc agcaatattt tctttcatat
     3481 ttcccatttt ttttacatat ccaaccgaaa tttctgcctc attttgccct aatatttcgg
     3541 ctggaatacc ggttatcatc gttccataac tcttgaccat atgtttgatt ttttcatgaa
     3601 catgcaaacc cgttttttca agcttggaag ttgtcatatt aatctcactg atagataccc
     3661 tatatttaat tgtagattaa aataaacttt gcacacttta gtttttttat ggtttatttg
     3721 tatttttttt atattttggt tgacgataag agcttaaatc aatagggcga ctattgaaga
     3781 aaaattcctg agagggggat cgaaccacta aagcaagcgc tgtaatggct gaaagagatt
     3841 catctttttg ttggtggttg caagcaatta tcgaaaccag gctttcctaa ttagtaaggt
     3901 ctcaaatcct cgattataat tagtaatagg gttaattggc tattcatacc ctgtgttgaa
     3961 aattgcacat tggattcatg gccagcctcg cgaagacttg cgtcatgctt tatagtcaat
     4021 aggccttaca tagaggaagg aggatgctct gtgcgaaggg aaaatacgaa gtcaaatatt
     4081 gattttaaat ccatatttga ttccctgccg gatttatacc ttttattgga cagggatttt
     4141 aatatcgtcg gagtcagtga tgcctactta caggcaacaa tggtgaatcg caaacaaatt
     4201 ttgggaaaaa atgtcttcga tgcttttcct gaaaatccaa acgatccaaa ggcaacaggt
     4261 gctaagaacc tatatgcatc gcttcacagg gttctaaaaa acaaatcagc agatacaatg
     4321 gctatacaaa aatatgatat tcagagacct gcagaccgag gagggggatt tgaagaacgt
     4381 tattggagcc ctattaatac tccggtttta ggggctgaac aggaagtgga atatattatt
     4441 catcgtgtgg aagatgttac tgaatttgtc catttaaaaa aaaccggttc agcgcaactg
     4501 aagctgatgg aggaactgcg taccaaggct ggggaaatgg agattgaaat ctatcagcgt
     4561 gcccaggaaa ttcaggaagc aaacaagcag ttggaagagg ctaataaaaa tctggctaga
     4621 cttgatcaaa tcaaaaccca atttttcgct aatgtcagtc atgaacttcg taccccatta
     4681 atgctgatac taggttctct ggaaatgttg cttgctgaaa aaaggcttcc ctcatttgtt
     4741 ctgaacacat taaggcgcat acaaaataat gctcaaattt tactgaaaca tgtcaatgat
     4801 ttgctgaata tatcaaagct ggatgctggc aaaatgattc ttcattatgc caatattgat
     4861 ttggtaaaat taattcaaca gacaattgca ttgtttgagg ctcagattcc cgaacgcaag
     4921 tttgtttttt caatggattt gcctgatgaa ttacaggcac aaatggatcc tgacaaaatc
     4981 cagcatgttt taatcaatat tttgtcaaat gccataaagt tcacaccgca aaatgcgaaa
     5041 gtaaggatta gattagcgca aaccaatcct gaaacatgca ggcttcagat agaagataat
     5101 gggtcgggta ttccccccga tctttgtgaa actatattcg agcgtttttt tcaggttgag
     5161 gaacctacaa ctcgtacggt agggggcact ggattgggtt tatcgattgc aaaagatttc
     5221 gttgaattac atgggggctc aattctggca gggaatgctc gaaatggtgg tgccattttt
     5281 accattgaat taccactaaa agcgcctcaa aatgtaaagg tttattcttc ctatagccag
     5341 acattattgg gggaaaaaga aataagcact agtcttgctg aattcagaaa taaagtaaaa
     5401 ccaattaagg agagagctga ggaggatttt gaacaggatt attacgatag accgctgatc
     5461 ctggtagtag aagacaatca ggagatgaat cattttattt gtaaaatatt gaacactatt
     5521 tatcgtactg aaagtgcttt tgatggtcga gaggggttgc aaaaatcgct cgctttacat
     5581 ccagatctca tcgttagcga tattatgatg cctcatatga gcggtgtcga aatggtgcat
     5641 gctattagag aacacccccc gctattgaaa actccaatta ttattttaac tgccaaagca
     5701 gacgatgaat taactgcacg tatgctggaa gaaggggctc aggattatgt gataaaacca
     5761 ttctcggcca aggaatttaa agccagagtt gctaatttga ttttagtaaa gaaagccgaa
     5821 gatgaactgg atcaatttgt ctatcacgca tcacatgatt taaaagcgcc tctgcctgct
     5881 atggagcatt tgatctcatg gattgaagaa gatctgggtg aaatgctgtc tgaacaatct
     5941 aaaaaatatt tactgctctt gcgtcaacgt gcttcacgaa tgtcaaagtt gctcgatgga
     6001 ttgcttaaat acgctcaatc aggatatgtt tttgataagg ttcgtacaat tcacaccgat
     6061 agattgatta ctgatatcat aaagaaattg aatcctccgt agactgtcac gtttcaactc
     6121 gaagatacac taccagtatt gaatacttgt gagcttcctt tgagagaggt ctttacagcg
     6181 cttctcgata acagcatcag gcatcatcac cgcaaagacg gacatatcat cattggtaca
     6241 aaagaaaagg atcggtttta tgagtttttt gtagcggatg atggccctgg aatagaaaaa
     6301 aaatatcaag acagaatttt tcaattattt cagaccttac aacccaggga taaacttgaa
     6361 acaagtggtg taggtctaag cattgctaag aaaatagtcg agtttcaagg aggtaaaatt
     6421 tctgtggagt cagaaaaaaa caaaggcagc gtctttcgtt ttttatggcc aaaagataag
     6481 gagattggcg atgcgtgcta agaatgatga gtgtacccat gttttattgg tcgatgatga
     6541 tgaaatcgat ttaatgtctg ttcaaagagc gctgacaaaa ttgaatattc cggtttgttt
     6601 tcatatagct cgtgatggtg ttgaagctct cgaacaactc tctggaaata tcaaagcgac
     6661 caaacctaaa atcatcgtcc ttgatatcat gatgccaaaa atgaatggta tagaatttct
     6721 cagagaattg cgttctcata ttcattataa cccgattaag gttgttatct tcacgacatc
     6781 aagtaatatc cgggataagc aggcaacaac aggtttggat atagcaggat attttgtaaa
     6841 agactctcaa tttaatgagt ttatttattg ctgcaaacac ttgctggaaa aaaaatcaca
     6901 tctatcatag gccagacccg gtttttttaa tttaaaagct ttaacaaaca aggcacttgc
     6961 tctgtgctat tttttcttct tgcattcccc taatcaaggc gcgtatttgt tttttttcct
     7021 tatccttgaa ggctggattt gccagttgct ttttaagaag agagataggt gaatcaccat
     7081 agttatcttt aaaatttgga tcgaaagtgt attttctcca gaggtaattt gcagtttgat
     7141 atgcttttcc ttcgatagct gcaagtaaag gtgttctatt taagtggtag ctattggtct
     7201 gggctggatc gattcctttt tttagaatct ttttgacctc ttccaggtta ttgcttcttg
     7261 ccgccatcat aagatttccc catagctctc gctttatacg ttgttgtctt agttgatgca
     7321 gttccagatg tttatcatta gccaagcgat taaaaaattg acatgtctga gctatacgac
     7381 ctagtgtgtt tgcttttaag aaactcaatg tattgacaat tgtttcctca ggaagatcag
     7441 aaaaaaaatt ctttttcata gcagcaaata gtgatggtta aaagtttcat aattttacat
     7501 tatgattatt aattgaatat taagtcattg cttcactttt ttgttaacca ggatttaata
     7561 caagcagaac agaatcagaa taggcaaata caagatgcaa ttgtattttt atatatcaca
     7621 aagaattatt tatatgcaaa acaacctata atcaagttag tgtcagtttt gggatgtgaa
     7681 gacaggttat tatgattttg aatattacag cagcgcaatt cccggattta acgcttaatg
     7741 ctattgaatc ctgtcaaaat atctatcgaa gtattgattt taattttggg gaagatgcgg
     7801 atacggcaat taacaaagcc agtttggaga aatttatcaa tcaatttaag agtattcata
     7861 gtactcatga taaacctatt gaagggatta ttaccgtggg aaagatgaaa aatgtctccc
     7921 ctgatacagt gaaattactt cttactacag aagatttcgt tcaaatgctt gatcaaaaaa
     7981 gtttcttaaa attaatagta acttccaatg aaattgcaaa ttttgtattg gataatccta
     8041 aactaagagc aaaactggat ggcattgaac ctttggtcga tgcacaaaaa ttcgaaaatt
     8101 cttgtactgc aagagcaata atgaaaatac ttcttgaaag ggggcttatt gaaccaagca
     8161 gctatacacc tggcaaagaa ttggaaatat acaaagacat ttggctagaa cccggtaaag
     8221 tggcatcgcc tgaaaagatc gcatcctatt tttgcaaata taacttgaat gtcattggtg
     8281 tcgagattag agagctatca aaatctgtac gaaataaata ttctaaagat atggttatta
     8341 cttcactcta ttcacttttt aagaaagagg tgccaataag aaaaaaaatg acattgacaa
     8401 ccttaagtga ggcagatttc cctgagggga taaccactct tatcattata aaagctggag
     8461 tacttcatac cttattaggc aagaaacacc atgggcagtt tgaagtaacc gatccctggt
     8521 ttggtgataa aaaaatatat agtgggttca tggaatttct cgaaaaagag agaaaaaatc
     8581 tgggagtatt ttttgaaatt tctcaaggac cccaagagat ttttagaccg taatggttat
     8641 caatgtttgc caaaagcaaa tggccacatt attccatgca ggcaatgaaa agccttagtg
     8701 cgagcatcaa tagcaaaaga ttgattttta tggataacgt gagttatgga taagacagcc
     8761 attattttga atcgttcggg ctgaggaggc atttatgccg tctcgaagcc atgtatggaa
     8821 tttaatgttt cgtgccaagg cttcgagacg atgctacgca tctcctcagc ccaaacggac
     8881 tctggatatt gactgcttct tatataatcc ttatccagag cccacatcgg ataaatctga
     8941 aacaaggcat taaaaatcaa tctcttgcta ttgatgcccg caggtataga cctggatgaa
     9001 gcttgctgat taatttcttc ctgccattac tcccccatcg acattcataa tctcccctgt
     9061 gatccagctt gctttttctg acaaaagaaa ttcgattgca tcagcgatat cagttgcctt
     9121 gccgattctg ccgataggat gaaatttatt aaaatcctgt aacgactcct cgattctctc
     9181 ttcatcaata aatgatttat agataggagt ggctaccact gccggcgcca cggcgtttac
     9241 tcttatattg tgctcagcaa gctccatagc taaatgttgg gttagtgaat gaagtccggc
     9301 tttttgcatg gaataagcag aggagggggt tgctttaacc gcctgattcg cccacatcga
     9361 accgatattg acgatactac cccctccatt ttttttcatg ttttttacta cggcctgggt
     9421 gataaaaaag aacgccttat taagattaac ctgacgatcg taatcttctt cctcatgttc
     9481 taaaaaagtg atgggtttga aataaccagc ggcatttaca agatatttta ctcttcgagt
     9541 ttcatcactc atgctactca taaattggaa tacctgctct ttgtcataaa gatcaagaac
     9601 taaaatgtct acttttgctc ccagtttttg tatttctgcc gcggcatttt caagtttttg
     9661 tttatctttt ccggtaatta acagctccat attgttactt gaaagttttt ttgcagtttc
     9721 aaggcccata ccactagagc cgccaataat aatagctaaa ttttgattga acatggattc
     9781 tcctgattca ttaaaatact gtccaatcca gtatgaaaga tctacaatat taaaaaaagt
     9841 aagcactttt cagttcacta agtactttgt ggtacaaatt attgaaatgg atgaaaaaaa
     9901 aataatacaa aaaaaagctc ccattaagac tgcatcaatg gtagaggcta tctatggttg
     9961 taagtggtcc ttgactatct attctctttt acgaaatgga atcaatcgac caggggaaat
    10021 ggtacgaagc gttgaaggac tatcaacaaa agttttaaac caatgcttgc ggaagaatat
    10081 tgaatttggg attctcggca gaaatacctt tcatgagatc ccccccagag ttgagtactt
    10141 tgtgactcct tttggagaaa agtttttaaa aattctggat caaatagaag agttgaacga
    10201 gcaaatcagg gattatcaac aaatcgaatt ccacaataat gatagttaag atttgttata
    10261 aaagctccat tgctaatcga ctgcaagtga attatggctc acaatattca ccaacgtaat
    10321 gagacatcgc tagtccgtat cattattaac gccagaaaat atttacaata ggtattcaag
    10381 ttatggccag aaacgacccc attgcccttc taacaacatg gatttcagag gaattggaaa
    10441 gaggagcacc taatccacaa catgccacat tatctacggc aggcaaagat tctattcccc
    10501 atgggcgtgt tatcgccatt cgcgaaatca atgagcaagg cttattattt tttacccagc
    10561 gaggaaccag aaaggtggtc gaaatgacct ataatcctat cgtatctctg actttttggt
    10621 ttgagttatt tgaaagagaa gtcatgatag acggttcagc aaggccatta tcggaaagcg
    10681 aaaatcaagc atactgggat acctatccac ctttcgcgca aatcagattt catagttatg
    10741 cgcctacttc atcacaagtt atatccagta aacaactgat tgaaaataaa agaaaaatat
    10801 tgatttcccg gtttcacgat aagtcattgc ctatgagtga atattattgt ggtttcagaa
    10861 tcattcccac acgcatggtt ttttatactt atagaacgga tgaattgtcc gatgtcgtag
    10921 aatattgttt tataaaaaac aattgggaga agaatttgct ttctccttaa atatcaattt
    10981 gtttaagcct catgattgat gcaacaatat ttttttatta catccaatac ccattgcagc
    11041 ggtttatgat ttgataatcg tttatgatag taaaaattaa ctaaaaatgt ctttttaggg
    11101 aaaggc
//
LOCUS       DATNSN010000109        11106 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.109, whole genome shotgun sequence.
ACCESSION   DATNSN010000109 DATNSN010000000
VERSION     DATNSN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.109"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..862
                     /locus_tag="VAH76_002230"
     CDS             <1..862
                     /locus_tag="VAH76_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO1386835.1"
                     /translation="LENDTIKPSIEIGAFEALWANREVSSYKQLWEKLANSQDRYLSN
                     LVEYNCAEKFYKEAVKRLYNAGIKHFGVKISGTIDYPQGLEDADYPLALLYYIGNWDL
                     VFSNGISVVGTRHPSSEGIKRTIRLVKGLVEANYTIFSGLAAGIDAVAHRTAIESGGL
                     TVAVVGTPLWLKYPKENSELYSKIAKDFLLISQVPIVSYQQKDIKFNRLYFPERNKTM
                     SALSKATVIVEAGETSGTLTQANAALKQGRKVFILNNNFENPNLTWPHRLEKAGAIRV
                     RNIEDILKVL"
     gene            864..1502
                     /locus_tag="VAH76_002231"
     CDS             864..1502
                     /locus_tag="VAH76_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386836.1"
                     /translation="MKYILQQIDDSLLRDHLYLEANDKCYFYGEYAGRQGYAFSETNQ
                     LIFNFKKPMKARNKPDWQYKIKAIETISQILPASKGWAKLRQYTWVPIPPSKDRNDPE
                     YDDRLLQVLLKLKETEQCLDVREILLAQKSRNAAHAPDNQVRPTIKDHISNLVVDKAL
                     LTPAPKAIAVFDDVLTTGASFKAAQYILRKAFPSIPIIGIFVARNINLIDDI"
     gene            1604..1774
                     /locus_tag="VAH76_002232"
                     /pseudo
     CDS             1604..1774
                     /locus_tag="VAH76_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2145..3365
                     /locus_tag="VAH76_002233"
     CDS             2145..3365
                     /locus_tag="VAH76_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEO1386837.1"
                     /translation="MTENKRKGPLQDTTIRNAKAEKKPYKMPDERGLYLEVAPSGGKW
                     WRFRYRFEGKDKRISLGIYPDVSLKQARDRRDECRTMVANEIDPSEHRKAKKAAKIEN
                     AANSFEVVAREWFLKFSSSGLWSEGHADKIIQRLERDIFPWIGNIPISDINAPKLLSV
                     VRRIESRGALDTAHRALGNCGQIFRYAIATGRAERDPSQDLRSALPPVKETHFAAVTE
                     PKDVAKLLRALDDYEGTLIVKCALRLAPLVFVRPGELRSAEWEHIDFDTAEWRYKVTK
                     TDTLHIVPLSQQALDILKELHPQTGRDRYVFPSARDKKRPMSDNAILAAMRRMGINKE
                     EMTTHGFRAMARTILDEVLQFRPDIIEHQLAHAVRDPNGRAYNRTAHLPERKKMMQAW
                     ADYLDELKLRKSEL"
     gene            3387..4223
                     /gene="dinD"
                     /locus_tag="VAH76_002234"
     CDS             3387..4223
                     /gene="dinD"
                     /locus_tag="VAH76_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEO1386838.1"
                     /translation="MKTEIIHILTTNFESHAHKTDEGVEFWLARDIQKLLGYEKWDNF
                     LNVVSKAKTACEISEHNVADHFADVGKMVELGSGSQREINDIMLTRYACYLIAQNGDP
                     KKQEIAFAQTYFAYQTRKAELIEQRLLEAERVTARKKLSDTEKELSQVIYEQTNGNEN
                     FAVIRSKGDHALFGKSTQAMKKQWNVPDSRPLADFAPTIILKAKDFATEITIFNAKEH
                     GMTTEQAISKEHVTNNEAVRKTLIDRGITPEKLPPAEDVKKVERRLESEKKKSLKKPD
                     AL"
     gene            4244..5023
                     /locus_tag="VAH76_002235"
     CDS             4244..5023
                     /locus_tag="VAH76_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386839.1"
                     /translation="MKNERKFLDVASHAFIEATNGLLDLYHEQPAKITLTEEFSKIVL
                     NVYQNFMLDVLPSSQCLVDKKNFFSFAILCRSSLDIIIQILWILSLEEDEKLKAIEYF
                     LNFEGVDVGQNGKKIYEWQRVFRPTYSSKKTAMELGIDKECIILPLTQHKNKELKLTV
                     FDYLSKITHWNPRLLNDLVGCNSEKHLGNAVEHTRMAAISVETFIFCAITFTEIFLKL
                     CLKVDEESVNQKTSKIKEKFRGAFAPFYNDIEQETEVVIEV"
     gene            complement(5174..6964)
                     /locus_tag="VAH76_002236"
     CDS             complement(5174..6964)
                     /locus_tag="VAH76_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEO1386840.1"
                     /translation="MNIYGLVKVFLVALVIPAIGFAVSFFIINDVNQSLKSKGIGDVT
                     LFCEVLQSGQLEKKATAELQEACQEVTNIVLLGQGSVITAAIGILIPVLYWLASIITG
                     ESRKRIALLFPIVLRISVLLIAISVLLQGAILTYAVYIGESYFYGRIHPVLIGLIGLG
                     ALIGGLSLIVVAMSFGRKLKLSTMGKVLAASDAPQLFRFVRSLAEKLGAKEPQNIIVG
                     LEPTFYVTNSDIQVPGYEKQITGETLYISAPLARLLSKEEFASVVGHELGHFRGNDTI
                     YSMRFAPVYAGLGMALGALAIDENEGASGLAKVPAIAMLSYMYELFSTNVAKISRDRE
                     HKADEAGAEAGSALALATALIKVSLYSSLWNHARQENIERLNQGKIATNLSIVFQDTA
                     KYDIEHENIEQIMKSTLEQSISHPTDNHPSVSLRLKALGVNTETITKDMLLIPEDAAI
                     HLLDKSQKIEEEITLLEHKLMIAYGVASPPKEAERNHLLHATYSLAAAMISADGRVDL
                     GEVAVAEGIGKKLFEDFDSIEFREYCNNPEQIPDVVQLSEAMKEILEDEHKYLIIQYL
                     KAISETDGSVSKDEELLLQKVASGLGISIT"
     gene            complement(7332..7589)
                     /locus_tag="VAH76_002237"
     CDS             complement(7332..7589)
                     /locus_tag="VAH76_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386841.1"
                     /translation="MDDSIFVKGIEELKRLIATQYDLTVSAHKIENGKIIISLANKRK
                     NLVLNELNFRDAIEFLPNSPTNDKDKEDRHSLFLEIKNQLS"
     gene            7731..8255
                     /locus_tag="VAH76_002238"
     CDS             7731..8255
                     /locus_tag="VAH76_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386842.1"
                     /translation="MNYKKKDRIRVPENHAEKALTLGINSKLKLLVMSKASEWVELPP
                     GEIQSISNKHPTKALVIVVRAPMVNGEYVGNHPDLQELTYRDLWLYSSDYYLLEELEK
                     PTTGEALEQNQYWLQLRSKAGRAIEKFEPWAAKQQGKINITDDLKGWIKKEITSKAFE
                     IQTISKILQNHYKF"
     gene            8393..8593
                     /locus_tag="VAH76_002239"
     CDS             8393..8593
                     /locus_tag="VAH76_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEO1386843.1"
                     /translation="MAYKILKLPEVKERTAISRSRIYNLISEGLFPKPILLGGARAVG
                     WIESEINEWIEQQIDASRNQKC"
     gene            8727..>11106
                     /locus_tag="VAH76_002240"
     CDS             8727..>11106
                     /locus_tag="VAH76_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HEO1386844.1"
                     /translation="MFDTISQFRAAIETSGLVPPENIIPGKMHRFPGIGKTKENKAGW
                     CLLFLDQLGGCYGDWSTGLSEIWWGKRNQKLTPDEKAQFKCQVQEAQLKALNEAAAIH
                     QEAAKRALSIWNSSKPAEANYPYLLSKNIEPKGIRCYKGLLVTPLRADGKIYSLQFID
                     PEGNKQFLRGGRVAGCYFSLGNPRGADVICIVEGVATGKTINMATGYPVAVAFSSSNL
                     LAIAQVMSEQFPEHTLIICADDDADKEGNPGLTKANQAADILGAKVAIPYFGIDRPNK
                     ASDFNDMAAFSGLDAVAETIRAVLFQSDDNNWPEPFPLSEEINAQPYPLDALPNTVRN
                     AVEEVTAFTKAPTPLVASAALAALSLAIQAHVDVKRTEGLISPVSLYLLTIADSGERK
                     STCDSFFMKAIRDYESIAQEKAKPLIKDYQAEISIWEEKYRGVKEQIRILAKTNKPTH
                     EQELRLVNLEYEKPVSPLVPRLVYCDVTPEALKWNLAKVWPSGGILSSEAGTVLGSHG
                     MGTESVMRNLATLNQLWDGSPIPTERRGSESFTVNGARLTVGFQVQEVTLKHFLDRSN
                     GLARGTGFLARFLIAYPESTQGNRFFTEPPESWFNLAKFNTRISEILNQEVTIDKNGS
                     LSVIQVSLSPEAKKAWICFHDVIEGELGVGGELSEISDVASKIADNAARVAALFKVFN
                     QGVTGTINFDEFENASRIVAWHLHEAKRFFSGLALSDDQADIVKLDSWLVRYCHKKKT
                     NIVSRRDVQRNIVPIRLRDKKTLDIALNVLVEAKRIRQMQSGKKKGIYINPAL"
BASE COUNT         3647 a         1960 c         2200 g         3299 t
ORIGIN      
        1 tttggaaaac gatactatta agccctctat tgaaattggg gcttttgaag cactttgggc
       61 caatagagaa gtctcgtcat ataagcaatt gtgggaaaaa ctagctaact cccaagatcg
      121 atatttaagt aatcttgttg aatataattg tgcagaaaaa ttttataaag aggcagttaa
      181 acgtctctac aatgctggaa ttaaacattt tggtgtaaaa atttctggca caattgatta
      241 tcctcaaggg ttggaggatg cagattatcc attagcttta ttatattaca taggtaattg
      301 ggatcttgtc ttttcaaatg gtatatcagt ggttggtact cgccatccat cttcggaggg
      361 aattaaaaga actatccgat tagtgaaagg gttagtcgag gctaactata ctatattttc
      421 tggcttggct gcgggaattg atgccgttgc tcacagaact gcaattgaat caggtgggtt
      481 aactgttgct gtcgttggaa ctccactttg gttaaagtat cctaaagaaa attcagagtt
      541 atatagtaaa atagctaaag attttctttt aattagtcaa gttccaatag tctcatatca
      601 acagaaagat ataaaattta atcgtcttta tttccctgaa agaaacaaaa caatgtctgc
      661 actctcaaaa gccactgtga ttgttgaggc aggggaaact tccggtacat taacacaagc
      721 aaatgcagct ttgaaacaag gaagaaaagt ttttatctta aataataatt ttgaaaatcc
      781 gaacttaact tggccacatc gattagaaaa ggctggtgca atccgagtac gtaatataga
      841 ggatattttg aaggttctat gaagtgaaat atattctaca acaaatagac gattctttat
      901 tacgtgacca tctttattta gaggcgaatg ataagtgtta tttttacgga gaatatgctg
      961 ggcggcaagg ctatgcattc agtgagacaa atcaattaat ttttaatttt aaaaagccga
     1021 tgaaagctcg taataaacct gattggcagt ataaaattaa agcaatagaa actatctctc
     1081 agattcttcc tgctagcaag ggatgggcaa agcttagaca gtatacatgg gttcctattc
     1141 ccccctcaaa agatcgaaat gatcctgaat atgatgatcg tttgttgcaa gttttattaa
     1201 aattaaaaga aactgagcaa tgtcttgatg ttagagagat tctattggct caaaaaagcc
     1261 gtaatgcggc acatgctcct gacaatcaag tacggccaac cattaaagat catattagta
     1321 atttagtagt agataaagca ttattaactc cagctccaaa ggccattgca gtttttgacg
     1381 atgtcttaac tactggagcg agctttaaag cagctcaata cattttaagg aaagcattcc
     1441 cctcaatacc tattattgga atttttgttg ctcgtaatat caatttaata gacgatattt
     1501 gatgcgaagc gaggtttcaa ttttcactaa tgtcgatgag aatttaaatt ttcagagtca
     1561 attaatagca acggatattt gaattatttt gaacgagaga atcatggcta aaaatattaa
     1621 ttatcatgat tatttaattg aatccttaaa agaaccctct gaggcagctg ggtatttaaa
     1681 tgcagcgctc gatgatggtg atattgatgg ttttttagaa gcattacata atgtaataga
     1741 agcttatggt agtatgacta agttatcaga aaaatttcaa gagagataca atccaaactc
     1801 tgaaagcatt aggatctcat ttatgagtaa tgccaatgat tttgaagatc aaaagaatga
     1861 ttaatctctg ggaaaaacta cccaatccct gcccaaaaaa ataagcgata aaataaaact
     1921 atataatatc aatagcttat aaaaaattgt cgtgggttcg attcccgccg cctccaccaa
     1981 ttaatgattc agtaacatcc aataaagtcc taaagttcct tgtaaataaa ggactttcca
     2041 ctaaatagca tccaatatca tcctaagaaa tttattgaca gccatgggca actgtgggca
     2101 agatcgcgga caacagatgt cttgcgaaat agagttgccc aaaaatgaca gaaaacaaaa
     2161 ggaaaggccc actacaagat accaccatcc gtaatgccaa agcagagaaa aaaccctata
     2221 aaatgcctga tgagcgaggt ttatatctag aggttgcccc ttctggaggc aagtggtggc
     2281 gttttaggta tcgcttcgag ggtaaagaca aaagaatctc tttaggcatt tatcctgatg
     2341 ttagcttaaa acaagctcga gaccgcagag atgaatgtcg cacgatggtt gcaaatgaaa
     2401 ttgatcctag cgagcatcgc aaagctaaaa aggcagcaaa aattgaaaat gctgcaaata
     2461 gttttgaagt tgtagctaga gaatggtttt taaaattctc ctcttctgga ctatggtcag
     2521 aaggtcatgc agacaaaatt attcagagat tagaacggga cattttccca tggattggga
     2581 atattccaat ttcagatatt aatgcaccaa agcttttaag tgttgtgcgg cgaattgaaa
     2641 gtcgaggagc actagataca gctcaccgcg cattaggcaa ttgcggacaa atatttcgat
     2701 atgctattgc tacaggtcgt gcagagcgtg atccttccca agatttacga agtgcattac
     2761 caccagtgaa ggaaacacat tttgctgcag ttactgaacc taaagatgtg gctaaattac
     2821 taagagcttt agatgactac gaaggaacat taatagtaaa gtgtgcatta cgtttagctc
     2881 cattagtatt tgttcgccct ggggaattgc gttctgcaga gtgggagcat attgattttg
     2941 acacggcaga atggcgttac aaagttacaa aaacagatac tcttcatatt gtcccccttt
     3001 cacaacaggc cttagatata ttaaaagaac ttcaccctca gactggtcgc gatagatatg
     3061 ttttcccaag cgctcgggat aaaaagcgcc caatgagtga taatgcgatt ctggccgcta
     3121 tgcgtcgtat gggcattaat aaagaagaaa tgactaccca tggttttaga gcaatggcta
     3181 ggactattct agatgaggtt ttgcagtttc ggccagacat cattgaacat caattagcac
     3241 atgctgtacg agatcctaat ggtcgcgcat ataaccgaac agctcatttg cctgagcgca
     3301 agaaaatgat gcaggcgtgg gctgattatt tggatgaatt gaagttgaga aaatcagaat
     3361 tataaccatt atcaaaagga attattgtga aaacagagat tattcatata ttaactacca
     3421 attttgaatc acatgcacat aaaacagatg agggagtaga attctggcta gcaagggata
     3481 tacaaaaatt actagggtat gaaaaatggg acaacttctt aaacgttgtt tcaaaagcaa
     3541 aaaccgcgtg tgaaatatca gagcacaatg ttgcagacca ttttgccgac gtcgggaaaa
     3601 tggttgaatt aggttcggga agccagcgag aaattaatga cataatgctt acacggtatg
     3661 catgttattt aattgcacaa aatggcgacc ctaaaaaaca agaaattgcg tttgctcaaa
     3721 catattttgc ttatcaaacc cgaaaagctg aattaattga gcaacgcttg ctagaagcgg
     3781 agcgagtaac tgctcgaaaa aaattgtctg atacggaaaa agagctttct caagttattt
     3841 acgaacaaac gaatggtaat gaaaattttg ccgtaattcg tagcaaaggt gatcatgctc
     3901 tttttggcaa atctactcag gctatgaaaa agcaatggaa tgtaccagat agtcgtccat
     3961 tagctgactt tgctcccacc attattctaa aagcaaaaga ctttgctact gaaattacta
     4021 tttttaatgc caaggaacat ggcatgacaa cagagcaagc tatttcaaag gagcatgtta
     4081 caaataatga agcagttcgt aagacactta tagatcgggg cattacacct gaaaagctgc
     4141 ctccagctga ggatgtaaag aaggttgagc gtagactgga atctgaaaaa aagaaatctt
     4201 taaaaaaacc tgatgcattg taaaactaat taatgagaat aatatgaaga atgaaagaaa
     4261 atttcttgat gttgcttccc atgcttttat agaggccaca aatggattat tggatttata
     4321 tcatgaacaa ccagcaaaaa tcacattaac agaagaattc tctaaaatcg tactgaatgt
     4381 ttatcaaaat ttcatgttag atgttttgcc tagttcccag tgtcttgtag acaagaaaaa
     4441 ttttttcagt tttgccattt tgtgccgttc atcactagat attattattc aaatattgtg
     4501 gatattaagt ttggaggaag atgagaagct taaagccata gaatattttt taaattttga
     4561 aggagttgat gttggccaga atggaaaaaa gatttatgaa tggcagagag tttttcgtcc
     4621 tacgtattct agcaaaaaaa ctgcaatgga attgggaata gataaggagt gtataattct
     4681 cccattaacg cagcataaaa ataaagaatt aaaattaact gtttttgatt atttatccaa
     4741 aattacgcat tggaatccaa gactattaaa tgatcttgtt ggatgtaact cagagaaaca
     4801 cctcggaaac gcagttgagc atacaaggat ggctgctata tctgttgaga cctttatttt
     4861 ttgtgcaata acttttacag aaattttcct gaagctctgt ttgaaggttg acgaagaaag
     4921 cgttaatcaa aaaacaagta aaataaaaga aaaatttcgt ggagcatttg ctccgtttta
     4981 taatgatatt gaacaagaga cagaggtagt tattgaagtt taacttaatg acctagcgtt
     5041 cacgtagaat tggcagtctt ttgagctcat ttttataaaa ctgccattaa ggctacaagt
     5101 cgatgattat gagatggcta aatgggggac tacttcatga caaaataact ttccctccta
     5161 cactacgttt gggttatgtg atagaaatac ccaaaccaga agctactttc tggagcaaga
     5221 gttcctcatc cttggataca ctaccatccg tttcagatat agctttcaga tattggataa
     5281 tcagatattt atgctcatcc tccaatattt ctttcattgc ttcggagagc tgcactacat
     5341 cgggtatttg ctcagggttg ttgcaatatt cgcggaattc tatcgagtca aaatcttcaa
     5401 ataatttttt tccgatccct tctgcgaccg ccacttcacc gagatcaact cttccatcag
     5461 ccgaaatcat tgccgctgcg agtgaatatg tggcatgtaa caaatgattc cgctctgctt
     5521 ccttaggtgg gctggcaaca ccgtaagcta tcatgagttt atgttctaag agagttatct
     5581 cttcttcaat tttttgggat ttatccagca aatgtatcgc cgcatcttct ggaataagca
     5641 gcatatcttt tgtaattgtt tctgtgttaa ctcccaatgc cttaagacgt aatgatacag
     5701 acgggtggtt atccgttgga tgagaaattg attgctccag agtcgatttc ataatctgct
     5761 ctatgttttc atgctcaatg tcgtacttag ctgtatcctg aaatacaata ctcagatttg
     5821 ttgctatctt gccttgattg agtcgttcaa tattttcttg acgtgcatgg ttccaaaggc
     5881 ttgagtaaag agaaaccttg attagagctg tggcaagtgc caaagcagac cctgcttctg
     5941 ccccagcttc atctgcttta tgctcccgat cacgactaat ctttgccaca ttggtagaaa
     6001 ataattcata catataggaa agcattgcga ttgccggtac tttggcgagt ccactagcac
     6061 cttcattctc atctattgca agtgctccta gcgccatccc taaaccagca taaacaggag
     6121 caaatcgcat actataaatg gtatcatttc ctcggaaatg tcctaattca tgccctacaa
     6181 ctgatgcaaa ctcttctttc gacagcagcc ttgctaatgg tgcagaaatg tagagcgttt
     6241 ctccagttat ttgtttctca taccctggga cttgaatatc gctatttgta acatagaagg
     6301 ttggttctaa tcccacgatg atattttggg gctcttttgc gcctagcttt tccgcaagcg
     6361 aacgaacaaa tctgaaaagt tgcggtgcat ctgaggctgc gagaactttt cccatcgtac
     6421 tcaatttaag tttcctacca aaactcatag caactacgat aagacttagt cccccgatga
     6481 gcgctccaag ccctataagg ccaataagga caggatgaat gcgtccgtaa aaataggatt
     6541 caccaatata tactgcatat gtaagaattg caccttggag caatacagat atcgcaatta
     6601 acaagaccga gatacgaagt acaataggaa acagcagagc aattctcttt ctactttcac
     6661 cagtgattat tgatgctaac cagtaaagta ctgggatcag aattcctatt gctgctgtta
     6721 tcacacttcc ctgcccaagc aatacaatat tcgtcacttc ttggcaggcc tcttggagtt
     6781 cggctgttgc ctttttctca agttgaccag attgcaggac ttcgcaaaat agggttacat
     6841 ctccgattcc ttttgatttc aatgactgat tcacgtcatt aatgataaag aagctgaccg
     6901 caaagccaat ggcaggaatt accaaagcaa ctagaaatac tttcacaagt ccatatatgt
     6961 tcattgcttc cccagttctt aatacctttc gtcatcgatc acgaggcaac gataatttgt
     7021 ttccatttag aaaaagtata gtgcaagatt tacttttaat ttgatgcatg gttggtggat
     7081 aagcactatc taattggttt aattatttac gatttactag tatgtatcta gtattccgca
     7141 aagcacgctt atagcatttc acttttatcc cttgtaattc ataaatgcga ccattataag
     7201 tgattgagca tgtaagaaga gtatcgttta ttactgcata ttctttggct tgcaattcaa
     7261 tacaagtaga tctatttgga tcctggtata aagatgtatt ccagttcaga tacaacagcg
     7321 gaaaatagtt gttaacttag ctgattcttt atttccaaaa agagagaatg tctatcttct
     7381 ttatctttat catttgttgg actattaggt agaaattcaa ttgcatcacg aaaattcagc
     7441 tcatttagaa caagattttt tcgtttatta gctaaactaa ttataatttt tccattctct
     7501 attttatgag cgctaacagt tagatcatat tgggttgcaa ttagtctttt taattcctca
     7561 atccctttta caaatattga gtcgtccatt tcagctcctg gttattcgca aacatatctt
     7621 tcatccgcat cttctttaac tgtactgtaa aatacaaaaa ataccacttg caacctaaga
     7681 gttccctctg gaagaattgc caattcaata aaatggttcg gtggttaata atgaattaca
     7741 aaaaaaaaga tcgtataaga gttcctgaaa atcatgcgga aaaagcatta actttgggta
     7801 taaatagtaa acttaagctg ttagtaatgt ctaaggccag tgaatgggtt gagttacctc
     7861 ctggggaaat acaatctata tcaaataaac atccgacaaa agctctagtc attgtagtca
     7921 gagctccaat ggttaatggt gagtacgtgg gtaatcatcc tgacctacaa gaacttacat
     7981 atcgtgattt atggctatat tcttcagatt attatctttt agaagaatta gaaaaaccaa
     8041 ctaccggaga agcattagag caaaaccaat attggttaca gttaaggagc aaagctggcc
     8101 gtgccatcga gaaatttgaa ccttgggcgg ctaagcagca aggtaaaata aacataacag
     8161 atgatttaaa aggatggatt aaaaaagaaa taactagtaa ggcatttgaa atacaaacca
     8221 ttagcaaaat tttgcaaaat cactacaaat tttaacaaaa caaaatttaa tttatattaa
     8281 attcaataag ttatttttgt aaaaaattat ttgaaaataa atgatgtgtc aaatttacac
     8341 ttaatttaac tttaagccat acccgtcctt caacaactta ggagaattaa atatggctta
     8401 taaaattttg aaattaccgg aggttaaaga gcgaaccgct atatcaagaa gtagaattta
     8461 taatctaatt tcagaaggtc ttttccccaa acccattctt ctcggaggag cccgagcagt
     8521 cggctggatt gaatccgaaa tcaacgaatg gatcgagcag caaatagatg ctagccgaaa
     8581 ccaaaaatgc tgacagggag gaaagacaat gtctcagctc agagaaggtc gcattggctg
     8641 gaaccaagcg accttgatac taattaaatt acagcttgag tttatcgcaa tatggtgtga
     8701 cttcgccagt aaggggagaa aagaaaatgt ttgacaccat ttctcaattt agagcagcaa
     8761 tagaaacatc aggtttggtg ccccctgaaa atattattcc cgggaaaatg catcgttttc
     8821 ccggaatcgg caaaactaaa gaaaataaag ctggctggtg cctattattt ttagatcaat
     8881 tgggtggttg ttatggagat tggtcaacgg ggctatcaga gatttggtgg gggaaaagaa
     8941 atcaaaaact aactccagat gagaaagctc agtttaaatg tcaagtacaa gaagcgcagt
     9001 tgaaagcact gaatgaagca gcagctattc atcaggaggc tgctaaaaga gcgctatcta
     9061 tttggaatag ctctaaacca gcagaagcta attatcctta tcttcttagt aaaaatattg
     9121 agcctaaagg tatacgttgt tacaaaggat tattagttac ccccttacgg gcagatggta
     9181 aaatatactc gctacaattt attgatcctg agggtaataa acaatttctt agaggggggc
     9241 gagtagctgg ctgttatttt agtttaggta atccccgagg ggcggatgtt atatgcatcg
     9301 ttgaaggagt tgcaacagga aaaacaatta atatggctac aggttatccc gtcgctgttg
     9361 cttttagtag ctctaatctt ctagcgatag cccaagtgat gagcgagcag tttcctgagc
     9421 atacattaat aatctgtgcg gatgatgatg ctgataaaga aggtaatccc ggtttaacta
     9481 aagcaaatca ggctgcagat attcttggtg caaaagtagc tattccttac tttggtatag
     9541 atcgcccaaa taaagcatca gattttaacg atatggcagc ctttagtgga ttggatgctg
     9601 tggctgaaac tataagagct gttctgtttc aatccgatga taataattgg cctgagccat
     9661 ttccattatc agaagaaatt aatgcccagc catacccttt agatgcgctt ccaaatactg
     9721 ttcgtaatgc agtcgaagaa gtaacagctt ttaccaaagc tcctacgcca ttagtggcat
     9781 ccgctgcatt agctgcttta tctttagcta tacaagctca tgttgatgtt aagcgaacgg
     9841 aaggcttaat tagtccagtc agtttatatt tgctaacaat agctgattct ggtgagcgta
     9901 aatctacctg tgatagtttt tttatgaagg cgattagaga ttatgaatct atagcacagg
     9961 aaaaagctaa gcctttaata aaagactatc aagcagaaat atctatatgg gaagaaaaat
    10021 atcgaggtgt gaaggaacag attcgaatac ttgcaaagac taacaaacct actcacgaac
    10081 aagaactgag attagtaaat ttagaatatg aaaaacctgt atcccctcta gttcctcggt
    10141 tggtctattg tgatgtaaca cctgaagcgc ttaaatggaa tttagctaaa gtatggccgt
    10201 caggaggtat tctctctagt gaagcaggta cggtcttggg ttctcatgga atgggtacag
    10261 aatcggtaat gcgaaattta gctaccctta atcaattgtg ggatggatca cctatcccta
    10321 ctgagcgccg tggttctgaa tcatttactg ttaatggagc tcggttgact gttggtttcc
    10381 aagtccagga agtaacatta aaacattttt tagatcgctc taatggatta gcaagaggga
    10441 cagggtttct tgctcgattt ctaatcgcct atcctgagtc cacacaagga aatagattct
    10501 ttacagaacc accagaatct tggtttaacc tagcaaaatt taatacgcga attagtgaaa
    10561 tattgaatca agaagtgact attgataaaa atggcagcct ttccgttata caagtttctt
    10621 tatcacccga agcaaaaaaa gcatggatat gtttccatga cgttatcgaa ggggaactag
    10681 gtgttggggg tgaattaagt gaaatctctg atgttgccag taaaattgct gataatgcgg
    10741 cacgtgttgc agctttgttt aaagtattta accaaggtgt tactgggacg attaactttg
    10801 atgaatttga aaatgcatca agaatagtgg cttggcatct acatgaagca aagcgttttt
    10861 tttcaggact agcattatca gacgatcagg ctgatatcgt taaacttgat tcatggcttg
    10921 ttagatattg tcataagaaa aaaactaata tcgtatctag acgagatgta caacgtaata
    10981 ttgtgccaat caggttacgt gacaaaaaga ctcttgatat agctttgaat gtgttagttg
    11041 aggctaaaag aatacgtcaa atgcaaagtg gaaaaaagaa agggatttat atcaatccag
    11101 ctttgt
//
LOCUS       DATNSN010000110        10967 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.110, whole genome shotgun sequence.
ACCESSION   DATNSN010000110 DATNSN010000000
VERSION     DATNSN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.110"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            124..5889
                     /locus_tag="VAH76_002241"
     CDS             124..5889
                     /locus_tag="VAH76_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEO1386845.1"
                     /translation="MNKNPFSLIMNVLCSIFSTLFGKIKWNHPTWLVYLKHKSKNSPQ
                     IFWGTTLLFIALLGAGAYGAYWYNNLPKPTYTTASITIPDTTPNEENLVPNNLIIDFG
                     IRSNEFIPQSVAPINLIGKEIDEGIEMAPQIAGKWSWNSDSQLIFIPAEDWPAGQKYT
                     IHFAKNFFAPNANIESYDLSFTTKPFQATIREFKFYQDPVNAETRHAVATIEFNYPVN
                     PKNLEKNTSVVFQTIKNGKADLTAESVPFSYTYDNNKRIAYLHSANIKIEDVSRYLLL
                     TLNKNVSSSTDSAILGQSISKNLLIPDASNFLKIVSTSANIVRNDKDRPEQVLSIETS
                     LGVNESEFNKSVHVYLLPQNYPATAAEPEKSNYEWRNPGEVTNNILSLSTPLPKEVIP
                     SEQNYTTLHGFKFKAQTPRYLYIKIDKGMKGFGGFKLSNDYTAIVPVPEYPKEISFLH
                     KGSLLALSGEKKLSVLIRGVPAVKYEFARVLPDNVNQLVTQTQGDFSNPYFINPSFNQ
                     QNISQIFSEIQQYDSTDLTRQQYTSLDLGKYLASEANASGPQGLFLLQATGWDVANQY
                     ALDVKASRLILITDLGMVVKDNNDGSHDVFVSSITQGLPVAGATVTILGKNGLPILSR
                     VTDEQGRANFPTLKDFIEDREPTVYLANLNNDVSFIPFNNYNRQLNFSKFDIGGIYTN
                     DQDLQNLTAYLFSDRGIYRPGDTVHVGMIVKRAFAEPQPSGLPLQATVVDARGTTIKD
                     QKVTLNDTGYMELDFTTSTNSPTGQYMVYLYLVKDNHPQNLLGSTSVRISEFQPDRMR
                     ITSSFEPKPFDGWSSPTDLKAKVDLWNLYGAPAANRRVSGKILLTPQRVQFDNYPDYI
                     FADPLYDPKKPPKVFTENLAEATTNENGQAEFNLNLERFEKATYQLTFFAEGFEPGSG
                     RSVTTQAKTLVSPLPYFIGYKADGDLHFIKQNSVRNVNYIAINPQLGKEDVKNLKIQL
                     ISLRPVTTLVKKADGTYQYQSTIQSTVINTNPFEIKESGVNYTLPTNTIGDYSLNVLD
                     KDDSILSQLQFSVVGTSQAPLAKNAELSIKLNKEEYQANEDIEIQITAPYTGSGLITI
                     ERDKVYAVHWFKTEAQNSVQTIRIPADFQGNGYVNVAFIRDWESPELFISPLSYSVAP
                     FNVNHDKHDIKIGLKTPELARPGESFTIDYHTDKPGKIIVFAVDEGILQVANYTTPDP
                     LSFFFQKRALEVLTQQTVDQILPKFIQDREISAVGGDEGEAALASRLNPFKRKTDLPV
                     VYWSGILDTDSTDRQLTYQIPDYFNGSIRVVAVAVTNDSVGSREVSAEVRGDFIISPN
                     VPTFVAPGDEFEISATVANNIKDSGEHAQVEVELTASSELEVIGSAKESLEISEGNEQ
                     TVYFKLRAKSLLGSARIAITAKSGDKSSKMDSTLSVRPANPFTTYINSGKSQDPQKSL
                     EVIQTLYPEHRKVDATISTSPLVLIFGLQRYLDNFPYGCTEQLTSKALPWLVMNSLPW
                     FSSESGPIHEKINTTIQMLSQRQMSNGGFSYWPGLSDNSANSFASVYAMHFLTEARAQ
                     GFNVPGEMFYNGINYLKELASRNPTDADSARIQAYAIYILTRNELVTTNYLANLQLYL
                     QQDSSKNWLKDITAVYIAATYQLLKSYSEANQLIGQYQPPTNNAFTTDFYDRNIADAQ
                     YLYLVAKHFPNLLPQVSDNILMNLVEAINNDEINTVLSGFSSLALGAYPQNESVDLSD
                     KLSITEVLANNQQRNIPTDQNNYQKLNIDETVKTIRFNNPDKQTFFYQLVQSGFNKII
                     PAEPLKQGIEIYREYRDEKGNVIDSAVLGEEIEVHLQVRSLDNNYLSNIAIEDLLPGG
                     FEVVRDSVKTDNMDYADVREDRVNFFGWVDLTAKELVYKIKATNVGKYIVPPAYAEAM
                     YNPNTKARGSSSVITVSREED"
     gene            complement(6158..7519)
                     /locus_tag="VAH76_002242"
     CDS             complement(6158..7519)
                     /locus_tag="VAH76_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1666 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386846.1"
                     /translation="MGKKAYIIGMGPAGLASALALLAKGYSVELIDRRSEDDVFSRKQ
                     GVFLKDDTIRDFFALSQLASKGYSLEFKDNFICKLIRPIDESNLKLSAEDQLDLAFFE
                     LIEKERTVIPIAELQRYQYSKLKYIYDKGGFTFQDEEDPYTVKFNNAEQQLIFHLGDT
                     QITKVDAENQELTLDHNGSIQNHSFDLLVDASGKTSKSFAQLWNSQNPEFAIGYEKLD
                     NPDHNAFGVMYLSINNPPLAMIANPVLSPIEGTSFIPLDKITPLKAMGWTHDYPPLIY
                     LKYSKKNGAVYLTGEIPESILKDNNKKELKQWFDLVLQLLFNNTNFKTSAPGLASVFK
                     TDIEIADKFALLLPKGGAFCLVGDALMSANFLLGCGISNALDDANQLPDMVDNQFSQS
                     EAEAYRNLRYLDYKDDWLFFREHIALRLKSLQLEKQLDLSRQELELKQRELENIQQQC
                     SFY"
     gene            complement(7722..9113)
                     /locus_tag="VAH76_002243"
     CDS             complement(7722..9113)
                     /locus_tag="VAH76_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1667 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386847.1"
                     /translation="MEYKEVIPMIRFFIICFIFLVSHLAYANPYWDRYKVPRDSHYPN
                     LKFHYINPDSLKIVQENAGIDVLRIQTDWFEETDSYQVGTVIIESSGTPALLSRSIHK
                     PKWGSYLGILKSRFGGNTVYYDAIGTGKEYRKLARAINLRFPVPSHDMIFELYAENPQ
                     TGLMEKVVEKNIIASELTRDNRHFNDLEVRQLLPAVKTPSLRVNIYAEGYSQNEKMKF
                     WNRAIKTVQALQSQQFPGIDYMDFYAVFNPSYKRLGVAEDLGLPVPEFDSFLGLYYPY
                     WDNFGRWYHIVYPTRENKFRQGLASAPYDYPIVLVNNSQYWGVGNYKSHTAIPADNST
                     YFTYILIHELGHFFGLNEEYEGGGRTELEFAPEMKEPWSQNITFLTTPSYSGLKWNDL
                     VNPQMSIPTPYSDWQSTPPQYGAYRGGYGDSPSHQGASHKPGLNCVMESHASFCDICQ
                     KGIMDVIRFDLGT"
     gene            complement(9260..10084)
                     /locus_tag="VAH76_002244"
     CDS             complement(9260..10084)
                     /locus_tag="VAH76_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386848.1"
                     /translation="MKKIAIILSLFPFLICCGLANATASQKRLPGESYQDAVKRIINN
                     KPQNELKMLIVRGTPYSKKVSLQKIDYSTVPKVSSYDELMNMFNLIRDTRFLYSPDKP
                     DFQRRISWLYPDDGCFARAALSRIKLDSEHFVIPAKIFVFGDLEMQTPYSSEGTVSWW
                     YHVSAVVNYMGTIYVLDPAAKPEGPMLIDDWYNKIGIMDKMEAVVCNPYTYDPFDRCY
                     DATNKTDNKALNDQLKYLDKEWIRIESLGFDPSVLLGDNPPWIGDMGSSTLLSYKS"
     gene            10365..10922
                     /locus_tag="VAH76_002245"
     CDS             10365..10922
                     /locus_tag="VAH76_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386849.1"
                     /translation="MASDNPFSGLREKMLNLYHQAERDLFREPVFRDQFQGATLDDVI
                     ISRASVKFQWGKISEDQRAHLQGLFLAYLEDQASSIYKEYFDKKFGSGPNTTTRSLLD
                     QFESKKITSLENIRAVLDKCVTENKEEDYWFTNAARGILLTNAFAISESSSESYQSEL
                     EQRISKLIPPPGNANEEEQQKIYSP"
BASE COUNT         3587 a         2175 c         1966 g         3239 t
ORIGIN      
        1 gaattggcaa aggatagact aataattata aattaataat ttattgcggg gggtgtatgc
       61 tttaccgcac cctcattacc agatcctaaa ataaacttag accaaccagg actccatatc
      121 accatgaaca aaaacccatt ctccctaatt atgaatgtat tgtgttcaat attttcaact
      181 ctattcggaa aaataaaatg gaatcaccca acctggttag tttatttaaa acacaaatca
      241 aaaaattctc cccaaatatt ttggggaaca actctattat ttatcgcgtt attgggcgcc
      301 ggagcatatg gtgcttattg gtataataat ttgccaaaac caacttatac tactgcatcc
      361 atcacaatac cagacaccac acctaatgag gaaaaccttg ttcccaataa tctcattatt
      421 gatttcggca ttagaagtaa tgaatttatc ccacaatcag ttgcccccat taatttaatt
      481 ggcaaagaaa ttgatgaagg gatagaaatg gccccgcaaa ttgcgggtaa atggagctgg
      541 aatagtgata gccaattaat ttttattccc gctgaagatt ggcctgcagg gcaaaaatat
      601 actattcatt ttgcaaaaaa tttctttgca cccaatgcga atattgaaag ttatgattta
      661 tcctttacaa caaagccctt tcaggccaca atccgcgagt tcaaatttta tcaagatccc
      721 gtcaatgcgg aaacaaggca cgctgtagcc actatagagt tcaactatcc agtaaatcca
      781 aaaaatcttg aaaaaaatac ttccgttgta ttccaaacca ttaaaaatgg aaaagcagac
      841 ctcactgcag aatccgttcc tttttcttat acttatgata ataataaacg aattgcctat
      901 cttcattcgg caaatatcaa aatagaagat gtatcgcgtt atttactttt aactttgaat
      961 aaaaatgtta gttcttcaac tgattcagca attcttgggc aatcaattag taaaaatttg
     1021 ctgatacccg atgcaagtaa ctttctaaaa atagtttcaa catcggccaa tatagtccgt
     1081 aatgataaag acagacccga acaggtactt agtattgaga ccagtttggg tgttaatgaa
     1141 agtgaattta ataaatcagt acatgtctat ttgctccctc aaaattatcc tgctaccgct
     1201 gcagaaccag aaaaatccaa ttacgaatgg cgaaatccag gtgaagtaac aaataacatt
     1261 ttgtccctgt cgactccatt acccaaagag gtaatccctt ctgagcaaaa ttatactacg
     1321 ttgcatggct tcaaatttaa agctcaaact cccagatacc tgtatatcaa gattgataag
     1381 ggaatgaagg gttttggtgg ttttaaatta agcaatgatt acacggctat cgtgccagta
     1441 ccagaatacc ccaaagaaat cagtttctta cataagggct ctttgttagc actgagtggt
     1501 gagaaaaaac tgtcagttct gatacgcgga gtacctgcag ttaagtatga attcgcgcgt
     1561 gtgttgcctg ataatgtcaa tcaactggtt acgcaaaccc aaggcgattt cagcaatcct
     1621 tattttatta atccgtcttt taatcaacaa aacatcagtc aaattttctc ggaaatccag
     1681 cagtatgact ccactgattt aaccagacag caatacacat ccctggatct gggaaaatat
     1741 ttagcatctg aagccaatgc ctcaggaccg caaggattgt tcttattaca agcaacaggt
     1801 tgggatgtag ccaatcagta tgctctcgat gttaaggcca gtcgtttgat attgattacg
     1861 gatctgggga tggtggtaaa agacaataat gatgggagtc atgatgtctt tgttagctcc
     1921 attacacaag gactcccagt tgcaggggca acagttacga ttttaggtaa aaatggcttg
     1981 cccattttat ctcgtgttac agatgaacaa gggcgagcaa attttcctac tctgaaagat
     2041 tttattgaag acagagaacc aaccgtgtat ttggcaaacc tgaataatga tgtgtctttt
     2101 attccattca ataactacaa caggcaattg aatttttcaa aatttgatat tggaggaatc
     2161 tataccaatg atcaggattt gcagaactta acagcttatt tattttctga tagaggaata
     2221 tataggcccg gagacacagt gcatgttgga atgattgtaa aacgagcctt tgctgaacca
     2281 caaccttctg gtctgccatt acaagccact gtagtagatg ccaggggaac tactatcaaa
     2341 gaccaaaaag tgacattaaa tgacactggt tatatggagc ttgattttac taccagtacc
     2401 aattccccca ctggccagta tatggtttat ttatacttgg taaaagataa tcacccgcaa
     2461 aatcttctcg gctcaaccag tgttcgtatt tccgaatttc aacccgacag aatgcgcatt
     2521 acttcaagct ttgaacccaa accttttgat ggctggagct cacccactga cctgaaagca
     2581 aaggtagatc tttggaactt gtatggtgcc ccagctgcga atagaagggt aagcggcaaa
     2641 attttattaa ctcctcagcg agtacaattt gacaactatc ctgattatat ttttgcggat
     2701 ccactgtatg atccgaaaaa gcccccaaaa gtttttacag aaaatctggc agaagcaaca
     2761 actaacgaga atgggcaggc tgaatttaac ctgaatcttg aacgttttga aaaagcaact
     2821 tatcaattaa ctttttttgc tgaaggcttt gagcctggta gtgggagaag tgttactaca
     2881 caagctaaaa cgttggtcag ccctttaccc tatttcattg gctataaagc tgatggcgat
     2941 ttgcatttta ttaaacagaa cagtgtcaga aatgttaatt atattgcaat taatccacag
     3001 ttgggtaaag aagatgtaaa aaatttaaag atacaattaa tttccctgcg ccccgtcaca
     3061 actctggtta aaaaagctga cggcacttat caatatcaat ccacaataca atcaacagtc
     3121 atcaatacca atccattcga aattaaagaa tctggtgtta actacaccct gccaacgaat
     3181 actattggtg attactctct caacgtatta gataaggatg atagtattct tagccagctg
     3241 caatttagtg tagttggtac cagtcaggct cctctcgcta aaaacgcgga attatctatc
     3301 aaacttaata aagaggaata tcaagctaat gaagatatag aaatccaaat cactgctcct
     3361 tatactggct caggcttgat cactattgaa agagataaag tctatgctgt gcattggttt
     3421 aagacagaag cccaaaattc tgtacagacc atccgcatac ctgctgattt ccagggtaat
     3481 ggatatgtga atgtggcgtt tattcgtgat tgggagtctc ctgaactctt cattagccca
     3541 ttaagctata gtgttgctcc attcaatgtg aaccatgata agcacgatat aaaaattgga
     3601 ctaaaaacac ctgaattagc ccgtcccgga gaatctttta cgatcgatta tcacacagac
     3661 aaacccggta agatcatcgt atttgcagtg gatgaaggaa tattgcaggt tgctaattat
     3721 acaacacccg atccactcag tttctttttc cagaaacgag cattagaagt actaactcaa
     3781 caaacggttg atcaaattct acccaagttt atccaggata gagaaatatc tgctgttgga
     3841 ggtgatgagg gtgaagcggc tttagcgagt cgtctcaatc cattcaagag aaagactgac
     3901 ttaccagttg tttactggtc cggtattctt gacacagaca gtactgacag gcagttaact
     3961 tatcaaatac ctgattattt taacgggtcc attcgggtag tcgctgtggc agtcactaat
     4021 gactcagtgg gatctcgcga agtttcagca gaggttcgcg gtgattttat tataagtccc
     4081 aatgtgccta ctttcgttgc acccggagat gaatttgaaa tatcagccac tgtggcaaat
     4141 aatattaaag attcaggaga acatgctcaa gttgaagtag aacttaccgc atcatcagag
     4201 cttgaagtaa tcggtagtgc gaaagagtct ctggaaatca gcgaaggcaa tgagcaaact
     4261 gtgtatttta aattgcgcgc taaatcactg ttgggatcag caaggattgc tatcactgcc
     4321 aaatcaggag ataaatcaag caagatggat agcacgctca gcgtcaggcc tgccaatccc
     4381 ttcacaacat atattaatag tggcaaatca caagatcctc aaaaatcgtt agaggttatc
     4441 cagactttat atccggagca tcgcaaggta gatgccacta tatctaccag tccattagtt
     4501 ctaatctttg gattacaaag atatttggat aatttcccat atggttgtac ggagcaattg
     4561 acaagcaagg ccttgccctg gctggttatg aacagtttac cctggttcag ttcggaaagt
     4621 ggcccaattc acgaaaaaat taataccaca attcaaatgt taagccaaag acaaatgtct
     4681 aatggcgggt ttagctattg gcctggtcta agtgacaatt cagcgaatag ttttgcatca
     4741 gtttatgcca tgcatttctt aactgaagca agagcacaag gattcaacgt ccccggagag
     4801 atgttctaca atggcattaa ttaccttaaa gaattggcta gtcgcaatcc cacagatgca
     4861 gactcagcaa gaattcaagc ctacgctatc tatattttaa cccgtaatga attagtcact
     4921 acaaattatt tagccaattt gcaattgtat ttacagcaag attcttcaaa aaactggcta
     4981 aaagacatca cagcagttta cattgcagcc acctatcaat tattaaaaag ttatagtgaa
     5041 gcgaatcaat tgataggcca atatcaacca ccaacaaata atgcatttac gactgatttt
     5101 tatgatcgca atatcgcaga tgcgcaatat ctctatcttg ttgcaaaaca ttttcctaat
     5161 ttactgcccc aagtaagcga caatatactc atgaatttgg tagaagcgat aaataatgat
     5221 gaaatcaata cagttttatc aggattttct agcttggctt tgggcgctta cccacaaaat
     5281 gaatcagtag atctatctga taagctgtcc ataacagaag tactggcaaa caatcagcag
     5341 agaaacatcc ctaccgatca aaataattat caaaaattaa atattgatga aactgtcaaa
     5401 acaatccgct ttaataatcc tgacaaacaa acattctttt atcagcttgt tcagtcaggg
     5461 tttaataaaa taataccggc agaaccttta aagcaaggca tagaaatcta cagagaatat
     5521 cgggatgaga aaggcaatgt cattgattca gcggttttgg gtgaagaaat tgaagtacac
     5581 ctgcaagtcc ggtcattaga taataattat ctgagcaata ttgccattga agatctcttg
     5641 ccaggcgggt ttgaggtcgt tagagactca gtaaaaacgg acaatatgga ttatgccgat
     5701 gtccgggaag acagggttaa cttcttcggc tgggtagatt taactgcaaa agaattagtt
     5761 tataaaataa aagcgactaa cgttggtaaa tacatagttc caccagccta cgcagaagcc
     5821 atgtataacc caaatacgaa ggcaagaggt tcttcatcag tcattacagt gtccagagaa
     5881 gaagattaat tgctggcaag caaagtcaga ttttgattct tgactttgct tgctaataaa
     5941 tacctaatta attgccctca tatttgctca tgccgagaaa tgaaggagat acttatcata
     6001 gcctgaggat gatataagac tctttcggga ggaatccatt ttgtagcaac actattggat
     6061 cttagagaat agattcctca gcccggcata gtaatgactt aagttttgca ttatatagtc
     6121 aaagtatggt tttgccctgt cgcagggagg ataatgacta ataaaatgaa cattgttgct
     6181 ggatattctc caactctcgt tgcttcaatt ctagttcctg cctacttaaa tccaactgct
     6241 tttcaagctg tagagacttt agcctgagag caatatgctc cctaaaaaac aaccaatcat
     6301 ctttataatc taaatagcga agattacgat aggcttcagc ttcactttgg ctaaattggt
     6361 tatcaaccat atctggtaac tgattagcat catccaaagc attgctaatg ccacaaccca
     6421 ataaaaaatt ggcggacatc aaagcatctc caaccaagca aaacgcacct ccctttggaa
     6481 ggagcaaagc gaatttatca gcaatttcaa tatctgtttt aaaaacagaa gctaaaccag
     6541 gggcgctcgt cttaaaattc gtattgttaa ataataattg caataccaag tcaaaccatt
     6601 gttttaactc ttttttatta ttatccttga gaatggattc aggaatttcc ccagtgagat
     6661 aaacagctcc attctttttt gaatatttca aataaattaa aggtggataa tcatgcgtcc
     6721 agcccatggc ctttaacgga gtaatcttat ctaaagggat gaagctggtc ccctcaattg
     6781 gacttaacac cggattagct atcatcgcca aaggcggatt attgatcgac agatacatca
     6841 caccaaaagc attgtgatca ggattatcta atttttcata accaattgcg aactctggat
     6901 tttgcgaatt ccacaactga gcaaatgatt ttgatgtttt tccggatgcg tcaaccagca
     6961 aatcaaaact gtgattttgt atgctaccat tatgatccaa ggttaactcc tgattctcag
     7021 catcaacctt ggtaatttgc gtgtctccca agtgaaaaat gagctgctgc tctgcattat
     7081 tgaattttac agtataagga tcttcttcgt cctgaaaagt aaaaccacct ttgtcataaa
     7141 tatatttcaa cttactatac tgataacgct gcaactctgc gataggaatt acagtgcgct
     7201 ctttttcaat taattcaaaa aaagctaaat ccaattgatc ttctgcactc aatttcaagt
     7261 tggattcatc gatgggacgg attaatttac agataaaatt atctttgaat tccaaggaat
     7321 accctttgct tgccagttga cttagagcaa aaaaatctct gatggtatca tcctttaaga
     7381 atactccttg tttgcgacta aaaacatcat cttcggatcg gcggtcaatt aattcaacgg
     7441 aatacccttt agctaataat gcgagagcac tagccaagcc agccggcccc atgccaatga
     7501 tatatgcttt ctttcccata agttggactc tactacttaa aataaatgac aaattttagc
     7561 atttaattgt taagaaatta ttaggtgagc atttctattg ccagtgcccc gctgtgcagg
     7621 acaaaactca ggtagactct ctttttgtat aggaagagta gcatgttgat tattaatttt
     7681 tctgaactct gatctggggg ttccaacctg aaacccctaa tttaggttcc caaatcgaaa
     7741 cgaattacat ccataattcc tttttgacaa atatcgcaaa atgatgcgtg agattccata
     7801 acacaattca agcctggttt atgacttgca ccctgatgag atggtgaatc tccatagccg
     7861 ccacgatatg ccccatattg cggaggggtt gactgccagt cactataggg agtaggaata
     7921 gacatttgtg gattgactaa gtcattccat tttaatcccg aatagcttgg agtagtcaga
     7981 aaagtgatgt tttgtgacca cggttccttc atctcagggg caaattccaa ctcagtccgt
     8041 ccaccacctt catattcttc attgagccca aaaaaatgac ccaattcatg tatcaaaatg
     8101 taggtaaaat aagttgagtt atcagcaggt attgctgtgt gtgatttata attacctaca
     8161 ccccagtact gactgttatt aaccaatact attggataat cgtaaggtgc tgacgccaat
     8221 ccctgacgaa acttgttctc tcgtgtagga tatactatat gataccaacg tccaaaatta
     8281 tcccagtatg gataatataa acctagaaaa gaatcaaatt ctggtactgg caaacctaaa
     8341 tcttcagcga ctcccaaacg cttataagat ggattaaaga ctgcataaaa atccatataa
     8401 tcgataccag gaaactgttg actttgtaat gcctgcacag ttttaatcgc tcgattccaa
     8461 aatttcatct tctcgttttg agaataccct tcagcatata tattcactct taaagaggga
     8521 gttttcactg cgggtaatag ttgcctcact tccaaatcat tgaaatgacg attatctctt
     8581 gttaattcac tagcaatgat atttttctca acaacctttt ccattaaacc tgtttgcggg
     8641 ttttctgcat aaagttcaaa gatcatatca tgggagggaa cggggaaacg caaattaata
     8701 gcacgtgcca gcttgcgata ttccttacca gtaccaatcg catcataata gaccgtatta
     8761 ccaccgaatc tgctttttaa aatacccaaa taactccccc atttgggttt gtgaatactt
     8821 cgtgataata atgccggtgt tccagaggac tctattatta ctgtaccaac ctgataagaa
     8881 tctgtttctt caaaccaatc ggtttggatt cttaatacat caattcctgc gttttcctgc
     8941 acaatcttca aagaatcagg attgatgtaa tgaaacttta aatttgggta atgactgtct
     9001 ctgggcactt tatagcggtc ccaataggga ttggcgtatg ccagatggga aaccaaaaaa
     9061 ataaagcaaa tgataaaaaa tcttatcatg ggtatgacct ccttgtattc cagaactact
     9121 atcccatact aacttgtaga ttttcctaca aatgttaacc gagtttaacg aacactcttt
     9181 tatctatcga acttgatatt ccgccagatt gaacgaaagc gtgttaattt ttgggtatcg
     9241 taacaacgat tcacaaaaat catgatttat aagacaaaag agtagaacta cccatgtcac
     9301 ctatccatgg aggattatca cctaataaaa cagacggatc aaaacctaat gattcgattc
     9361 taatccactc tttatccaaa tatttcaatt gatcatttaa cgctttatta tctgttttat
     9421 ttgttgcatc atagcaacga tcaaaaggat cgtaggtata gggattgcaa actaccgcct
     9481 ccattttatc cattatccca attttgttat accaatcatc aatcaacatt ggaccttctg
     9541 gtttggcagc gggatcgagc acataaatgg tacccatgta gttgacaaca gctgacacat
     9601 gataccacca acttactgtt ccctcagaag agtatggtgt ttgcatttcc aaatcaccaa
     9661 aaacaaaaat ttttgctgga ataacaaaat gttcactatc cagtttaatt ctggacagtg
     9721 ctgctctggc aaagcaacca tcatcaggat aaagccaact gattcttctt tgaaaatcag
     9781 gtttatcagg tgaatacaaa aaacgcgtgt ctcgtatcag gttaaacatg ttcatcaact
     9841 cgtcataact gctaaccttt ggcacggttg agtaatctat tttctgtaaa gagactttct
     9901 ttgaataagg agttcccctg acaattaaca tcttcagttc attttgtggt ttgttattta
     9961 tgatacgttt cactgcatcc tgataagatt ctcctggaag ccgtttttgt gaggcagttg
    10021 cgtttgccaa gccgcaacaa atcaaaaaag gaaataatga taaaataatg gctatttttt
    10081 tcatagcaat ccttgagtat ttataatatt ggttgatatt ttaccaatgt ttgtcgtcct
    10141 tgacattctt tataattagc atgaactggt tgaaaagact agtctattag tcatatgaac
    10201 tatcaatcgt caaaacataa tatatcaaaa acataatggc ttatattttt taccaaggta
    10261 agcattcaag atgctgaatt taattgatct gaccttacaa taagtttctc ataataatga
    10321 tctataattc agatatacat caatttataa attaaaggct agtcatggct agtgataacc
    10381 ctttttcagg cttaagggaa aaaatgttaa atctctatca tcaagcagaa agagacctgt
    10441 tccgtgaacc tgtatttaga gaccaatttc agggtgctac gcttgatgat gttattattt
    10501 ctcgagcaag tgtaaaattt caatggggta aaatctcaga agaccagcgc gcacatttac
    10561 aaggtttatt tttagcctat ttagaagatc aagcatccag catttataaa gaatattttg
    10621 ataaaaaatt tggctcagga cctaatacca ccacccgctc attgcttgat caatttgaat
    10681 caaaaaaaat tacaagcctt gaaaatattc gagcagtttt ggataaatgt gtaacagaaa
    10741 acaaagaaga ggattattgg tttactaatg ctgcaagagg aatcctacta actaacgctt
    10801 ttgctatttc cgaaagctca agtgaatcct atcaatcaga attagaacag agaatcagca
    10861 aactaattcc acctcctggc aatgcaaatg aggaagaaca acaaaagatt tatagcccgt
    10921 agttgttata tgaaataggt gtgacttaga aaataatcat attaacc
//
LOCUS       DATNSN010000111        10878 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.111, whole genome shotgun sequence.
ACCESSION   DATNSN010000111 DATNSN010000000
VERSION     DATNSN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.111"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..533)
                     /locus_tag="VAH76_002246"
     CDS             complement(<1..533)
                     /locus_tag="VAH76_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HEO1386850.1"
                     /translation="MYPFILKPKSLRFFNLVFIALALSVCLLANARAAGIERYNFGDM
                     ASKVTKAWDQDVALYVAKRFAVENSFDEKEITVKKLNAGATNHFIYLMFINNKLKFVI
                     KGLGTEKEAEALFALQNYSKIPEIQNNPQAATVTLSKIINSYRVKNDSRTYFFAILEA
                     AQGKELFKIMTYELLQH"
     gene            941..1381
                     /locus_tag="VAH76_002247"
     CDS             941..1381
                     /locus_tag="VAH76_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386851.1"
                     /translation="MNKNIVLGIALFGAAMTASASDNLCGQLNISIYNKSGHACHIEN
                     YDLKGGQFLYGQVPQTIKNNEVASKFTISQDDKTAPRIVLKYRCENKNFIIESSQPWC
                     SIFGGAGSVSGIVQHSDNVQVSYTTIDGSYLHSTAGQIDWHIGG"
     gene            complement(1566..1961)
                     /locus_tag="VAH76_002248"
     CDS             complement(1566..1961)
                     /locus_tag="VAH76_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386852.1"
                     /translation="MFGSKIKAFQRDAEKHPLTKKEQANELLLLNTILNKLNIAIPAL
                     IRIINTYYVPLKLSDFTSPEPFISQLDELKKQMEPSETLPDRSEGKVTTKRNSMKRHS
                     IHSLERHSLFRPGNEPPISGNGTDLKPNI"
     gene            2424..3473
                     /locus_tag="VAH76_002249"
     CDS             2424..3473
                     /locus_tag="VAH76_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO1386853.1"
                     /translation="MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSR
                     IVPNLITQADPGDLFSIRNIGNIIPPYPSSYSEAGAIEYALKVLEIKDIIICGHSNCG
                     AMQGLLTPHIEEHLPGVASWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQM
                     EHLKTYPLVIEKTAHKELTIHGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKN
                     KIVSGLCMNYLQQMIPLKSEKEYYRLMELITNLQEDILSIWEFIREPAKQQLWKELGG
                     FYASPEDEEFIAMVESSTSIKLTELEEFKKKIFFANNNTATSLLLNSSIFKKPTKDIG
                     KLNAEPVISCNPPLK"
     gene            3527..3655
                     /locus_tag="VAH76_002250"
                     /pseudo
     CDS             3527..3655
                     /locus_tag="VAH76_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3668..3734)
                     /locus_tag="VAH76_002251"
     tRNA            complement(3668..3734)
                     /locus_tag="VAH76_002251"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3708..3710),aa:Phe,seq:gaa)
     gene            3886..5118
                     /locus_tag="VAH76_002252"
     CDS             3886..5118
                     /locus_tag="VAH76_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEO1386854.1"
                     /translation="MENIKAIKLTKTVVDKIKPVEGKDQTFYRDEQLKGFALRVTSSG
                     VKSFVVETRVAHKVKRITLGKYGQITAEEARKQAKHLLGQIAKGDNPIAEKKINKIKS
                     ITLKEAFHDYLKARKDLKPLTIKDYQSILKQVISDWFDKPLMNITREMIAKRHAQYGQ
                     TNSKARANYAMRVLRAVFNFAAHEYQLEDGQPIIAINPVEYLSHTRSWYRVDRKNTMI
                     KSHQLAAWHQGLTRLGEQEDYSQAMMWKDYFLLVLFTGLRRTEAASLCWKDVDFKDKT
                     FTVQDTKNREVHTLPMSDFLYELFLRRKQFKMNEFVFPSPSKTGHVIDPRKAMLKVAE
                     LSGVSFTVHDLRRTFATTAESLDLPAYALKRLLNHKMNTDVTAGYIVRDVERLRKPMQ
                     RISDFLVRQMSCDLGNNG"
     gene            complement(5285..6565)
                     /locus_tag="VAH76_002253"
     CDS             complement(5285..6565)
                     /locus_tag="VAH76_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1386855.1"
                     /translation="MFERKLTPIITELLAEFRIVYLTGPRQAGKTTLTKIIAQNSGLE
                     YISFDNQTILQSAQNDPIGFINSLHNKKVVLDEFQYIPELIPAIKQASDNLPPNVKGK
                     FLLTGSADIFRSGKTQEALPGHMARLELYPLSLSEQYKTDYNIVDLLCNENFNLNNLT
                     LLTRADLANFILKGGYPEIQDKSSRAKQIWYKSYIEGRLFKDFETLYHAKGDYRSKLE
                     ALIPYLAGISGNLLKYSNISNDLGEDDKLVKAYIEVLELMFIIKRVPAYLKNKAKRQA
                     ITIPKLQTIDTGLACSLLGIKNEGQLLNSLHYGGLLENLIYMELLKQNSWSEEKVELL
                     HFRDKYQNEVDIVMERDNHQIIGVEIKASATVNMHDFKGLIKLAEFNPSKFQCGVIFY
                     SGKEILPFSKNEIKLFALPISLFIKSDTKKDDVQ"
     gene            7135..7407
                     /locus_tag="VAH76_002254"
     CDS             7135..7407
                     /locus_tag="VAH76_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1386856.1"
                     /translation="MNKKPSRLQLLNEFESAPTSALFNQHTLAAVLDCSTQLLERNRW
                     EGKGVPYLKIGRKVLYRKSDVLSFLQQQKIYRSTCDEGQVLSVANQ"
     gene            7478..9229
                     /locus_tag="VAH76_002255"
     CDS             7478..9229
                     /locus_tag="VAH76_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEO1386857.1"
                     /translation="MLQNQIITLKNKNGRNPITCEYSGGCFKLNEQGVSFLGKDKDGN
                     PMAPRWICSPLFVVAKTRDAKSGEWGRLLEWQDDDGVIHQWAMPLALLQGDASEVRRE
                     LASLGLTISPHKISRDLLTTYLQVFPVEDRARCVEKLGWHENLFVTPSQIIGHSCEKI
                     VFQNSHAIESAMSVSGTVDDWRESIGQLASGNSRLIFAISAAFAPALAKIAGEDSGGF
                     HFRGASSSGKSTALKVAASVWGNPQAYCRLWRSTTNGLEGLAALHNDGLLILDELSQM
                     EPKEAGEAAYLLANGQGKTRASRHGTAKPSSRWSLFFLSAGEESLMSLMARAGQKTNA
                     GQEIRLADIEADAGFNMGLFEKIHNQLSPATMALSLKEYSSKYYGAVGMAWLQQVVAN
                     QQSIATDIADAVQEFVNSAILPDSTGQIIRVARRFALVAVAGELATQYGLTGWNEGES
                     TDAAYKCYRAWLEHFGMEGNKEDRTILAQVRAFFESHGSSRFDNIREPNNERIQNRAG
                     FFYTDDAGFRMYMVLTEVFKKELCQGFEPRTVVRVLINEGWLKPATDGMPTHKPRIKG
                     VGTPRVYVFTNKIWGGE"
     gene            complement(9329..10138)
                     /locus_tag="VAH76_002256"
     CDS             complement(9329..10138)
                     /locus_tag="VAH76_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO1386858.1"
                     /translation="MRTQSLRQFANRQIKQDRQGKYLYRKHRAYVIHKMIDDLFVIRQ
                     MPPSWQVLQSEQVHKLVRYWKKQNINPVTIMRYMTIIRRFLQMNNCPVANIDNQSLEL
                     SRPKTRQKRKKTISPDIWKLLHDPITRIIMGLQTEFGLTFKEAILIKPRIQVRECSIG
                     ITRDIAFNSIDRSIPIRTESQKAVLNLFNWLTKQYGNLLQLKSYEEIRIIWRSALAKH
                     CLSSSKSWRYLYAKQMYSHLLPDYGNYKTCWLIRDEMGIKSRNTLWLYLKN"
     gene            complement(10393..10533)
                     /locus_tag="VAH76_002257"
     CDS             complement(10393..10533)
                     /locus_tag="VAH76_002257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386859.1"
                     /translation="MNRWKSDETHRRLMLSHSHSLTLWLDVESGNPSGTFAVGKGSSS
                     EL"
BASE COUNT         3354 a         1924 c         2185 g         3415 t
ORIGIN      
        1 ttatgctgta gcaattcata agtcattatc ttaaataact cttttccttg agccgcttct
       61 aaaattgcga agaaataagt cctcgaatca ttcttcactc tatatgaatt aatgatttta
      121 cttaatgtaa cagttgcggc ttgtggatta ttttgaattt caggaatttt tgagtaattt
      181 tgtaaagcaa acaatgcctc tgcctctttt tctgttccca gacctttgat aacaaacttc
      241 aacttgttat tgataaacat caaatatatg aaatggttgg tcgcccctgc atttaatttt
      301 tttactgtta tctctttttc atcaaaactg ttttctaccg cgaaacgctt ggcaacataa
      361 agcgcaacat cttgatccca agctttagtc acctttgagg ccatatcccc aaagttataa
      421 cgttcaattc ctgccgctct ggcattagct aataaacaga cagacagggc taaagcaata
      481 aaaacaaggt taaaaaaacg caaggatttg ggcttaagga tgaatgggta caatttactt
      541 agccagtgaa ttaaatttaa gcatattgta tatctgttgc attcccaaat caattaatta
      601 gaaaagccaa ttggtttggt tttatgtggc gtttttagta aatccaatct tattacagaa
      661 gggtttcatt gaaaaatgat tattaaagga atattcccgc atgcattatg gtgctgtggg
      721 tatggctgca acaggtggta gcaaaccaac aaagcattgc aactacccat tgctaatgcg
      781 gtgcaggagt ttgataggaa ctaatttgac aagaatcact tttcaacatc acaggaatat
      841 acatcagcat acatacattc aaaattgcaa ttagtttatg cttagtatac attttgcagg
      901 cacattcaat atttattgta ataattctat ggagtataaa atgaataaga atatagttct
      961 tggtattgct ttgtttggtg ctgcgatgac agcctctgca tcagataatc tttgtggtca
     1021 attaaacata tctatataca ataagtctgg acatgcatgc catatagaaa actatgacct
     1081 aaaaggtggg cagtttcttt atggacaagt gcctcaaaca attaaaaaca acgaagtggc
     1141 ttctaagttt acaataagtc aagatgataa aactgcccca aggattgtat tgaaatatag
     1201 atgtgaaaat aaaaatttta tcatcgaatc tagccaacca tggtgttcga ttttcggagg
     1261 tgctggttcg gtgagcggaa tagtccaaca ctcagataat gttcaagttt catacactac
     1321 aatcgatggc agctatttgc attctactgc tgggcagata gattggcata ttggcgggta
     1381 attatatagt tgtaaacacg ctcttatgat ctacttttac agagttttgt ctaaataacc
     1441 attcccagac tcacagttca atattgcctt tgcatatgat tgtgagtcta tatgattacc
     1501 atttttcagc tatgagtagc taaagtcctt ttcagcgatt tgttcaattg ctgaaataat
     1561 gctttttata tattaggctt caagtcagta ccgtttccag aaatcggagg ttcattgccg
     1621 ggtctaaaga gactatgcct ttccagggaa tggatgctgt gtctcttcat tgagtttctt
     1681 tttgttgtta ccttaccctc agagcgatcg ggtaatgttt ctgaaggctc catctgtttt
     1741 tttaattcat ccagttgtga tataaagggc tcaggggagg taaaatcaga taactttaaa
     1801 ggaacataat acgtattaat tattctaata agtgcgggaa tcgcaatgtt taatttattt
     1861 aagatcgtat tcaacaagag taattcattt gcttgctctt tctttgttaa tggatgtttt
     1921 tcagcatctc tttgaaacgc tttaattttg gaaccaaaca agtcaagtaa taaagagggt
     1981 atcttgtcac agtggtaggt tttcttatta gtaactattg atggtatcag attagttgat
     2041 aaataatctc tgattatggt atctaaacct acatctaaac tttcagttgt gttatccctt
     2101 taaaatcaat tacattagaa agtatttaga tttttaactt ttattgtgtt ttaaaataat
     2161 ctgtctacaa atacagggtg gacagttctt actttgcctg gttcttttca aaccagagga
     2221 agtaaaccgg tgtgtatcga taccttcaag cgtttactta ttaaggacta aaaaatcaat
     2281 agcaagtata aatactgttt gtaaaaacag gccaaattcc tatacatttg tttacgatat
     2341 tgcaacattg ctttacgatt ctttgttgtt atgataattt gccttcatat gatggattct
     2401 gtaaagaaat tatgaggttg attatgttaa ttaaactgat gctcggatta cttgaattta
     2461 aaaaaaaacc ttttattggg atgagagatt tgtttgagca attaagcacc gggcagaatc
     2521 cagaaacact atttataacg tgctctgact caaggattgt tccgaatctt ataacacaag
     2581 cagatccagg tgatttattc agcatacgta atataggcaa tatcattcct ccttatccat
     2641 caagctatag tgaagccgga gctattgagt acgctttaaa agtattagag ataaaagaca
     2701 ttatcatttg tggccactcc aattgtggtg caatgcaagg attattaact ccgcatattg
     2761 aggagcattt gccgggcgta gcatcgtggc taaatcactc tcaagctgtg ttacaagagg
     2821 tgcatgagga acgagcaact gaaactaata ctcccgctcg agaacttgag attgctacca
     2881 agaagaatat tctgctccaa atggaacatt tgaaaaccta tccactggtc atcgaaaaaa
     2941 cagctcataa ggagttaaca attcatggtt ggtattatga actggaaact ggaaaggtat
     3001 ttatttatga acccgattta aaagaattta ttaatctcga atatgcatta gaccgggctc
     3061 ttgagtccag aaaaaacaaa attgtgtctg gtctttgtat gaattacctt caacaaatga
     3121 ttccattaaa atcagaaaag gaatattacc ggttaatgga attaattact aatcttcaag
     3181 aagatattct gtcaatttgg gaatttatta gagaaccagc caaacagcaa ttatggaagg
     3241 agttaggggg cttttatgcc agtccggaag acgaagaatt tatagccatg gtagaatcaa
     3301 gtacatcaat aaaactcact gaattagaag agtttaagaa aaagattttc tttgccaata
     3361 ataataccgc gacaagccta ttactcaact catcaatttt taaaaaacca acaaaggata
     3421 ttggtaaact aaatgctgag ccagtcataa gttgtaaccc tcccctaaaa taggtgcatt
     3481 tctgattaga gttctccaag ttaaactatg tttgaggaga acaaaaatga aaaaatcgcg
     3541 ttatacagaa acacaaattg taaaaattct aaaagaagtc gaagcaggtc ggctggttaa
     3601 agaaatttgt cgtgaatatg ggatttcaga tgcaaccgaa agatccggaa agagccattg
     3661 attctagtgg tggccagagg cagaatcgaa ctgccgacac gaggattttc agtcctttaa
     3721 aatacctttt taacaccttt caactatttt taaatttctt tattatccct ttaaaatcaa
     3781 gtacattaga gcatatttag attttttaac ttttattatg ttttaaataa tctgtctaca
     3841 tggtgtctac atggaattta aaacgtaatt tttctaaggt gttttatgga aaatatcaaa
     3901 gcaatcaaac tcactaaaac ggtggttgat aaaatcaaac cagtggaagg aaaagatcaa
     3961 acgttttatc gagatgagca gttaaaaggc tttgcacttc gggtgacttc tagcggagta
     4021 aaaagctttg tggtagaaac ccgtgtcgct cataaagtga aacgtattac cctgggtaaa
     4081 tatggccaga taactgctga ggaagcacgc aagcaagcga agcatttatt gggtcagatt
     4141 gcgaaagggg ataatccgat cgctgaaaaa aagataaata aaataaaatc gataaccttg
     4201 aaggaagcat ttcatgatta tcttaaagcc cgcaaagacc tgaagccact gactattaaa
     4261 gattatcaat cgattttaaa gcaagtgatt tctgattggt ttgataagcc cctaatgaat
     4321 atcaccagag aaatgattgc caaacgtcac gcacaatatg gtcaaacgaa cagtaaagca
     4381 cgtgccaatt atgccatgcg tgtattgcgg gctgttttta actttgcagc acatgaatat
     4441 cagcttgaag atggtcaacc catcattgct attaaccctg tagaatattt gtctcatact
     4501 cgaagctggt acagggtaga tcgtaaaaac accatgatca aaagtcacca gttggctgca
     4561 tggcatcaag gtttaacaag actcggtgaa caagaagatt attcccaggc tatgatgtgg
     4621 aaagactatt tcttactggt tctcttcacc ggtttaagga gaacagaagc agcatcacta
     4681 tgttggaagg atgttgattt taaagacaag actttcactg tacaggacac caaaaatagg
     4741 gaggttcata cattgcctat gtctgatttt ctctatgaat tattcttacg aagaaaacaa
     4801 tttaagatga atgaatttgt attcccatcg ccaagtaaaa caggtcatgt catagatccc
     4861 agaaaagcta tgctcaaagt ggccgaatta tcaggcgtgt cttttactgt tcatgattta
     4921 aggcgaactt ttgctacgac tgcagaaagc cttgatctgc cagcttatgc attgaaacgt
     4981 ttacttaacc ataagatgaa cactgatgta acggcaggat acattgtgcg ggatgtagag
     5041 cgattgagaa aacctatgca acggatcagt gattttttag tgaggcagat gtcatgtgat
     5101 ctaggcaata atggatagct ttctgaggaa gcaaccatcg acaattactc gacagttctt
     5161 tagaagtaat ttgatggtta acaaatacat gcgtataatt atgttactac ttcccctagg
     5221 actcagctcc tagggcacag agcctcttcg gaggctcttg tttttttaga tatacactat
     5281 tgctttattg aacatcatct ttctttgtat ccgacttgat aaacaaactt attggtaagg
     5341 cgaataattt aatttcattc ttagagaaag gtaatatttc tttaccagaa taaaaaatga
     5401 caccacattg gaattttgaa ggattaaact ctgccaattt tattaatcct ttaaagtcgt
     5461 gcatgtttac agttgctgac gctttgattt caactccaat aatctgatgg ttatcccttt
     5521 ccatgacgat gtccacttca ttttggtatt tatcacggaa atgtaataat tctacctttt
     5581 cttctgacca actgttttgt ttgagcaact ccatataaat taaattttct aatagccctc
     5641 cataatgaag gctattaagt aattgccctt cattttttat acctaaaagg ctgcaggcaa
     5701 gtcctgtatc tattgtctgc agtttgggta ttgtaatagc ttgccgcttt gctttatttt
     5761 ttaagtaagc agggactctt ttaatgataa acatcaattc taaaacttcg atatatgctt
     5821 tgaccagttt atcatcttcg cctaagtcgt ttgaaatatt tgaatattta agtagattgc
     5881 ccgagattcc cgctaaataa ggaataaggg cttcaagttt agaacgatag tcacctttag
     5941 catgatatag agtctcgaaa tctttaaaaa gacgaccttc tatataagat ttgtaccata
     6001 tttgttttgc tcttgagctt ttatcttgaa tctcgggata tccacctttg agaataaaat
     6061 tggctaagtc ggcgcgggta agtagcgtta ggttatttaa gttaaaattt tcattacata
     6121 atagatcgac aatattataa tcagttttgt attgttctga taatgatagt ggatatagtt
     6181 ctaatctcgc catatgtcca ggtaaagctt cttgtgtttt ccctgatcga aaaatatctg
     6241 ctgatccggt taataggaat tttcctttca cattaggtgg caggttatct gatgcttgct
     6301 ttatggccgg aattaattca ggtatatatt gaaactcatc caaaacgacc tttttattat
     6361 gcaaagaatt tataaaacca atcggatcgt tttgagctga ttggagaatt gtttgattat
     6421 caaaggaaat atattcaagg ccactatttt gggcaattat ttttgttaaa gttgttttgc
     6481 ctgcttgccg tggtccggtt aaataaacaa ttctgaattc agctagtaac tcagtaataa
     6541 taggggttaa ttttctttca aacatcggga ttcatataat aggtaacata ttttcaatat
     6601 ataacatctc ttaaaaaata gctaattaaa acccgaatta tccagtataa aaatccgaat
     6661 tatccaatat gataacccga gaaatccaag gtgatattcc gcataatcca tatgggaatc
     6721 tttattgttg agcttaaaat tagattaaat tttatttaaa gcactttata aaaaattagg
     6781 tgggacattc gggacaattg ggacaaccgc ataggcactc ggttttctgt gtcccaatat
     6841 caatttttca cttgggacac atgggacaaa tttttctggc taaatttttt tagtaacaat
     6901 gaatatcatt taataacaat cagatcacaa ggctttaaag cgcttgacac ccttttaaat
     6961 tgcaagcaat acttctcgcg agctgtaaac ttttacgggt ccacggtcct gtaaaagtca
     7021 cggaaaatga aatatcacga ctattatccg gccggacagt caactgatat tcatcgagca
     7081 ggacgcatac cgtcggcatt aagctgaatg aataatcatt tgatgaggtt ctatatgaat
     7141 aaaaaaccat ctcgtttaca actgttaaac gaatttgaat ctgcgcccac ttctgcgttg
     7201 tttaatcaac acactttggc ggctgtttta gattgttcca ctcaactttt ggaacgtaat
     7261 cgctgggaag gaaagggtgt tccctatctt aaaataggcc gtaaggtttt gtatcgcaaa
     7321 agtgacgtat tgtcttttct ccaacaacag aaaatctatc gatctacctg tgacgaaggg
     7381 caagtccttt cagtagccaa ccaataaaac agatagaaat agattgttca aagagagata
     7441 ggggttctat ctttctgaac tctgctggag aaaagtgatg ctgcaaaatc aaattattac
     7501 gctaaaaaac aaaaacggcc gcaatcctat aacctgtgaa tactctggag gttgttttaa
     7561 attaaatgaa cagggcgtgt ctttccttgg aaaagacaaa gacggtaatc ccatggcacc
     7621 ccgctggatt tgttctccat tatttgtggt ggctaaaacg cgagatgcca aaagtgggga
     7681 gtggggtcga ttacttgaat ggcaggatga tgatggggtc attcatcaat gggcaatgcc
     7741 tttagccttg ttacaaggag acgcctccga agttaggcga gaattggcta gtcttggttt
     7801 aaccatttca ccccataaaa tttcccgtga tttgctgacc acctatttgc aggtctttcc
     7861 agtagaggac agagctcgtt gtgtcgagaa attgggttgg catgaaaacc tctttgttac
     7921 tccctcacaa atcattggac attcctgtga gaaaattgtt tttcaaaata gccatgcgat
     7981 tgaatctgct atgtcagtat cgggcactgt agacgattgg cgagaatcaa taggtcaact
     8041 tgcttcgggt aattctcggt taatttttgc catttctgct gcttttgctc ctgctttggc
     8101 aaagattgca ggtgaagatt caggtgggtt tcacttcaga ggcgcttctt caagtggtaa
     8161 aagtaccgca ttaaaagtag cggcttcggt ttggggtaat ccgcaggctt attgccgttt
     8221 atggcgcagt accactaatg gtcttgaggg gctggctgct ttgcataacg atggcctact
     8281 gattctggat gagttaagtc agatggagcc taaagaagca ggggaggcgg cttatttact
     8341 ggccaatggt caaggtaaaa cacgagcctc acgtcatgga acagccaagc catcttctcg
     8401 atggtcctta ttctttctct cagctgggga ggaatcctta atgtctttga tggcgagagc
     8461 aggccaaaaa acaaatgcag gacaagaaat acggttagcg gatattgaag ctgatgcggg
     8521 atttaacatg ggactatttg agaaaattca caatcaactt agcccagcga ccatggcctt
     8581 atccttaaag gaatattcca gcaagtatta tggtgctgtg ggtatggcat ggttgcaaca
     8641 ggtggtggca aaccaacaaa gtattgcaac tgatattgct gatgcggtgc aagagtttgt
     8701 taatagtgct atcttgccgg attcaacggg gcaaattatc cgtgtagctc ggcggtttgc
     8761 tttagttgct gtcgcgggtg aattagccac ccaatacgga ttgactgggt ggaatgaggg
     8821 agaatccacc gatgccgcgt acaaatgtta cagagcatgg ctcgaacatt ttggcatgga
     8881 gggcaacaag gaggacagga cgatattagc tcaagtcagg gccttttttg aatctcatgg
     8941 ttctagccgc tttgataaca tcagagagcc aaacaatgag cggattcaaa atcgtgccgg
     9001 atttttttat accgatgacg cgggttttcg tatgtatatg gtattaacgg aagtctttaa
     9061 aaaagagctg tgccaggggt ttgagcctag aactgtggtt cgtgtgttaa taaatgaagg
     9121 ctggttaaaa ccggcaacag atggaatgcc tactcataag ccaagaatca aaggagtagg
     9181 aacacccagg gtctatgtat ttaccaataa aatctgggga ggggaataag cctctggaaa
     9241 tttgtcccat tatttttaag tgatgggaca tattgggacg gctcaacccc agtgtttatg
     9301 cgctgtcccg cctgtcccaa atgtcccgct aatttttgag atataaccac agagtattcc
     9361 gcgattttat tcccatctca tcacgtatca gccaacacgt cttgtaattt ccatagtcag
     9421 gtagaaggtg agagtacatt tgtttggcat acaaatatcg ccagcttttg ctgctggata
     9481 aacagtgctt ggctaatgca ctgcgccaga tgatgcggat ttcctcatat gattttaatt
     9541 gaagcagatt accgtattgt tttgtgagcc agttaaaaag attgagtacc gctttctggc
     9601 tttcagttcg tatcggtata cttcggtcga tggaattaaa agcaatatct cgggttatcc
     9661 cgatagaaca ttcacggact tggatacgtg gtttaatcag aattgcttct ttgaaagtga
     9721 gtccaaattc tgtttgtaat cccatgatta ttctggttat aggatcgtga agtaacttcc
     9781 aaatatctgg agagattgtt ttctttctct tttgtcttgt tttaggtctt gagagttcaa
     9841 gtgattgatt atcaatatta gcaacaggac aattgttcat ctgcaaaaaa cgacgaatga
     9901 tggtcatgta gcgcatgata gtgactggat taatattttg ttttttccag taacgcacca
     9961 gcttgtgaac ctgctcagat tgcaaaacct gccatgacgg tggcatttgc cgaataacaa
    10021 acaagtcatc aatcatctta tgaataacat aggcacggtg tttccgatac aggtatttac
    10081 cttgcctatc ctgtttgatt tgtcgattag cgaattgtct taaggattgt gtacgcatct
    10141 tcccatccgc aaaaaattat taaaaaaaat aaattgccct gtgtattgtt tagtgttggt
    10201 cgagcggttc acactctgtt gaaacccggc aaaagccgaa aagctcgaag aaggggggca
    10261 aatcaaccgt tataaggcaa acgatctcgc cagtactgct agtttttttg tggttttttc
    10321 tcctcaataa ataaagtaaa tgaaagtgat aaagcagggc tttatctcaa atccatggtc
    10381 aaatgaaatg acttagagtt ccgaagaact ccctttaccg acggcaaagg ttcctgaagg
    10441 gtttcccgac tctacgtcca gccacaaggt tagggaatga gagtggctta gcataagacg
    10501 cctatgcgtt tcatcggatt tccaccgatt catcagtcat gcttcattta gaaaatagca
    10561 aagtgcagat ctggggacaa gagcattata aggaatattt tgtcccatct tatctggtgc
    10621 gtcaggacaa atggggtttt aaggtatagt gttggagggt tagtgatgtc gaatattcct
    10681 gcgtcactgc tggaaaatgg cagtgacgca ggaaaaatta taaacctatt gtacccatga
    10741 attactagtt tatgaaatat atcatcctgt gatgaagaag tagttttttt gaataaattc
    10801 attttaatta tcatcaggag gattatttat tttcttttac gctaatgatc tttatgccta
    10861 gactagatat cttagaat
//
LOCUS       DATNSN010000112        10865 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.112, whole genome shotgun sequence.
ACCESSION   DATNSN010000112 DATNSN010000000
VERSION     DATNSN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.112"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(305..1072)
                     /locus_tag="VAH76_002258"
     CDS             complement(305..1072)
                     /locus_tag="VAH76_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEO1386860.1"
                     /translation="MNPIKPPFTLNQYFAAWFVHVFTASAACIGVFSLYKIYQHDYVF
                     ALWLMAITVFIDAVDGSLARLVHVKSVLPKIDGALLDNIVDYLNYVITPCFFLLVKPG
                     MLPADYVVPIIAAITITSAYQFCQDDAKTPDHFFKGFPCYWNITVFYMYIFNTSMIVN
                     TVLLSLFCVLIFIPVKYVYPSRLDYLTESRILKILMHCCSALYGISSFCLLVNYPETN
                     KLWVSLSLGYVGMYLFLSFYRTYYPMFKAKITANNKD"
     gene            1416..2804
                     /locus_tag="VAH76_002259"
     CDS             1416..2804
                     /locus_tag="VAH76_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO1386861.1"
                     /translation="MDIQTVNPTTEEVLNNYTCLDFDSIDKKIAFGYEAYLNWKDSKF
                     DIRRSLMLNLAQILKDKKQELAHLMALEMGKPITAGRAEIEKCAWVCEHYAENAEQYL
                     MPRTVQTEMKTAKVCYRPLGIVFAIMPWNFPFWQVFRFSAPTIMAGNGAILKHAPISS
                     GTGNKIEELFRDAGFPSSLFQHLIVDNDGASHIIENQFVTAVTLTGSERAGQSVAAHA
                     GKNLKKSVLELGGSDPYLILEDADLDLAAQCIVASRLNNTGQVCIAAKRVIALQSIEK
                     ELISKIKELISKYKMGDPLDPNINLGPMARKDLRENLHSQVEKSVKQGAKLLLGGIIP
                     EGKGYFYPPTLLTDVKPGMTAFDEELFGPVIVIITAKNESEAISLANMSQYGLGAAVF
                     TKDLNKGEHIATYEIEAGTCFVNAFVASDPRLPFGGIKRSGYGRELSREGILEFVNTK
                     TIAISDPKHGKK"
     gene            2791..3279
                     /locus_tag="VAH76_002260"
     CDS             2791..3279
                     /locus_tag="VAH76_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1386862.1"
                     /translation="MVKNSKFIFISIKKQEMSCFEKDHLIANYAISSGKNGVGEKINS
                     GCTPRGWHKIHSILGIDLPINSVMVAREWTGEIYSESLATQFPERDWILTRILQLDGL
                     EPGRNKGGDVDTLYRYIYIHGTPDTTQLGIPGSHGCIRLNNDAIIELAAWATKNTLVY
                     IQ"
     gene            3331..8208
                     /locus_tag="VAH76_002261"
     CDS             3331..8208
                     /locus_tag="VAH76_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEO1386863.1"
                     /translation="MSYKFEEGKDAIVKSVVEKIKQTMTGEQTEFCAEFAKQFYGTVA
                     LEDLLEWEVDDLYGAAVNFWSLICERAPHETKIRIYNPDYERHGWQTTHTVVEVICED
                     MPFIVDSLRIVINRMGLTSHLTIHMGGIRVKRDSHNRVTEILPRNGGAARDDVLHEAP
                     IFIEIDRQTDPATLSELHKNFERALEDNRAVFEDWDKMRTKVREIIKELDTVPKTIDI
                     SEIEETKAFLNWMEDHHFTFLGLRDYDLVKKGKETILQPIPETGLGVLRQSLSKSNAR
                     SITAMGPEAQGLISSPRILVMSKTNTLASVHRDAYTDYIGVKRFNKKGEVIGERRIIG
                     LYTSAAYHTNPKHIPFLRHKVALIMKNSNLNPYSHAGKVLLNILETLPRDDLIQGTED
                     ELLEIAMGIFYMQDRKRIRLFARADVYKRFISCLVYVPKDRFNTELRYAMQKILADSF
                     NAEEITFSTQFSESVLARIHFIVRVNPKNLPDFDLKEIEQKLIEVGRSWIDDLQHHLH
                     EVYGEEQANALYSRYKNAFPISYSDTFSPRTAVYDIKHIEMLSPENPLGINFYKPLDE
                     SEKSFRLKVYQHDTTIPLSDVLPILEKLGLRAISERPYVLKFEDGKVAWINDFAMQYN
                     KESEFNIDEIKELFQNAFARVWFGDAENDGFNLLVLAAGLNWREVAVLRTYAKYFRQI
                     GFTFSQDYMETALNNNVSIAKKLVRLFEIRCNPHDSKRREDRYVALVAEILSDLDNVA
                     NLDEDRIIRQYVHAIGATLRTNFYQVDAQGNPKNYISIKLSSKLIPGVPKPYPMFEIF
                     VYSPRFEGVHLRCGKVARGGLRWSDRREDFRTEILGLMKAQQVKNSVIVPSGAKGGFV
                     PKQIPANATREEIMEEGISCYKLFIRGLLDITDNYKEGLIVKPQNVVFYDEDDPYLVV
                     AADKGTATFSDIANSISQEYDFWLGDAFASGGSVGYDHKKMGITAKGAWESVKRHFYE
                     LNRDIQNNDFTVVGIGDMAGDVFGNGMLLSRHIKLVGAFNHVHIFVDPNPEAEASFKE
                     RERLFNLPRSSWTDYDKKLISKGGGVFSRSAKSIPVSPEMQKVFGIKQTSIEPNDLIK
                     AILKADVDLLWSAGIGTYVKSSTESNTSVGDRTNDATRVNANQLRCKVIGEGGNLGLT
                     QLARVEYSLHGGKVYTDFIDNSGGVNCSDKEVNIKILLNNVVSAGDLTPKQRNELLSN
                     MTDEVAKLVLRDNFLQTRAISLTASQALRAIELHARYINELEKTGKIDRTLEFLPDEK
                     VLMEHKLMGKGLTQPGIAVLMCYSKTILKEQVLASEVPEEDYMNQILIGSFPKPLQER
                     FSKQMQDHPLRREIIATRLSNIIVNEMGFTYVYRLQDETGASVAAIVRAYMIARSVLN
                     LEAIWKQIEELGTKISAQTQVDMMMLYVRLSRRVTRWFLRTHRRSMSITQAIELYAKG
                     VDELKKSMPAVFGETGRIQYEEHYQERIKEGIPPQLAHELTVTRGLFAATDIIEIAYE
                     ENIKIPKVAEIYFGIGEFLDIAWLRTQIIVHTTENHWESLSREALRDDLDWQQRQLTA
                     AIMGFEPNNKDLQERLTRWGETHVSLIDRWNYILTALKSSTALNYTMFLVATRELLDL
                     TQTTLQSNLKMEESF"
     gene            8229..8777
                     /locus_tag="VAH76_002262"
     CDS             8229..8777
                     /locus_tag="VAH76_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEO1386864.1"
                     /translation="MEAKTASEYSTLSKLFHWIIALAVIVMLIAGFFLDEIPEQFQGV
                     AYMLHKSTGITILFLMILRFIWIHAKGKPALPASVKSWEKILSRFVQYGFYVLLLIMP
                     LSGWIMSVAADRIPNYFGLFKAPLPWIEPNKSLAESMAESHEIIAWILIAFIILHVLG
                     ALKHHFIDKDNVLKRMLPGKSK"
     gene            complement(8812..9801)
                     /locus_tag="VAH76_002263"
     CDS             complement(8812..9801)
                     /locus_tag="VAH76_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEO1386865.1"
                     /translation="MKTILEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDF
                     LGEKSIDFLQGQLTCDLRLVSDVSMIQGAQCNLKGRILSLLDIINWQGVKLVLPQDLI
                     EVTQHSLNKVALLSRVKITSNNNYKILGFYLQHSNDQIPQLLPLSSELYALSCTSHGC
                     VYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFL
                     PHRIGLHQTAYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKS
                     DEDIEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQLEEKIELEL"
     gene            9927..10166
                     /locus_tag="VAH76_002264"
     CDS             9927..10166
                     /locus_tag="VAH76_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEO1386866.1"
                     /translation="MDNREKSRLLWKCRRGMLELDLLLQKFIANEIDRLTENQLKAFD
                     NLLTHNDPNLYAWLMGHEEPEKELLEIVSFIRNCD"
     gene            10108..10551
                     /locus_tag="VAH76_002265"
     CDS             10108..10551
                     /locus_tag="VAH76_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386867.1"
                     /translation="MRNPKKSYLKLYHSSETVIKPGKSKLYLRLIVILYLCTIGLVLY
                     SSLPMLIGGVLIFLIVIQFKNDITYQSACQKVKEIKFLKNLTWSLETIDGTTHHYDKL
                     KILIHNMLFQVIQLSSDKKNQILILFNDQIPTDQLRLLHLNSNKK"
     gene            10653..>10865
                     /locus_tag="VAH76_002266"
     CDS             10653..>10865
                     /locus_tag="VAH76_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1386868.1"
                     /translation="MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTH
                     DWTNVSDLTQEVLIKVFRHLSYFNERS"
BASE COUNT         3722 a         1752 c         2112 g         3279 t
ORIGIN      
        1 aattaagatg ctattttaat cattaaatag acaaagtcta tcatggaata aaaaaatcaa
       61 acgcatagaa gctattaatt cgtctttatt atatctttgt tatcaggtat tcataagtta
      121 ttggcataat ttaacttcta tagaggcgta gaccttatat caattgcttg cattacctct
      181 ctattttttt cagtaaacag atactattta aagcaataag aaaagaaaaa ttcctaaaag
      241 agtacgataa acagtgccac cagagatcta atatctgcat ggaaaacata agttacatat
      301 aaactcaatc tttattattt gccgtaatct tagctttgaa catcggataa taagtcctgt
      361 agaaacttaa aaataaatac atgcccacat agcctaaaga taggcttacc cataatttat
      421 ttgtttcagg gtaattaact agcaggcaaa atgaactgat gccataaaga gcagaacaac
      481 aatgcattaa tattttcaag attctcgact ccgtcaagta atctaacctt gatgggtaaa
      541 catattttac tggaataaaa atcagtacac aaaaaaggga caagaggaca gtgttcacaa
      601 tcatagaagt gttaaaaata tacatataga agacagtaat attccaatag caggggaacc
      661 ctttaaaaaa gtgatcagga gttttggcgt catcctggca gaattgatag gccgaagtaa
      721 ttgtaatagc agctataatt ggcacgacat aatcagctgg cagcataccc ggtttaacta
      781 gcaagaaaaa acatggagta atgacataat tcaaatagtc aactatattg tctaataaag
      841 caccatcaat tttaggcaat acgcttttta catgaaccaa tcgagcaagg ctaccatcta
      901 ctgcgtcaat aaatacagta attgccatta gccataaagc aaatacataa tcatgctggt
      961 aaattttata taagctaaag acaccaatac aggcagcgct ggcagtaaat acatggacaa
     1021 accaggcggc aaaatattga ttaagggtaa agggaggctt tattggattc attacttatc
     1081 ctagagttaa tcaatgtcat ctactactaa ttagggcttc agtctttttt gtaaaagcaa
     1141 aaaacaaatt acctttaaaa atatccagtg caatgattaa atctggattg ctttgttcat
     1201 caagacgatg tgtttttgct ttttactaaa acagtgcaag tcctttttac ttaacaggtt
     1261 gatttttatt actgttcttt aagacaatgt cgtttaaaat aaaaatagta tcaatattca
     1321 aaagcaaagt gcaagttagg ttaattattt aaaataaaaa aaaagccttg atttggtcta
     1381 aactataggc aacgttaatc atatcgagta attaaatgga tattcaaact gtcaacccga
     1441 caactgaaga agttttaaat aactacacat gcttggattt tgattcaata gacaaaaaaa
     1501 tagcgtttgg atatgaggcc tatttaaatt ggaaagattc taaattcgac ataaggcgct
     1561 ctttaatgct caatcttgct cagatattaa aagataaaaa gcaagagtta gcgcatttaa
     1621 tggctttaga aatgggtaaa cccattacag caggtcgtgc tgaaattgag aagtgtgctt
     1681 gggtatgtga acattatgct gaaaatgcag aacaatacct aatgccaaga actgttcaaa
     1741 ctgaaatgaa aacagcaaaa gtttgttaca gaccactagg aattgtattt gccataatgc
     1801 cttggaattt cccattttgg caggttttta gattttcagc accaactatc atggcgggta
     1861 atggggctat tttaaaacat gccccaatat cgagtggaac aggaaataaa atcgaagaat
     1921 tattcagaga cgctggattt ccttcttcat tatttcaaca cttaattgta gataatgatg
     1981 gggcatctca tatcattgaa aatcaatttg ttaccgcagt tactttaact ggaagtgaac
     2041 gtgcggggca aagtgttgca gcgcatgcag gtaaaaattt gaaaaaatcg gttctagaat
     2101 taggcggttc agatccctat ttgattctgg aagatgcgga tttagatctt gctgctcaat
     2161 gcattgtggc ttcacgatta aataataccg ggcaggtctg cattgcggcc aagcgagtta
     2221 tagcacttca atcgattgaa aaagaattaa tttctaaaat caaagaactc atcagcaaat
     2281 acaaaatggg agatccatta gaccccaata ttaatttggg acccatggca agaaaagatt
     2341 tacgagaaaa tttgcattca caagtagaaa aatctgttaa acaaggtgct aaattattgc
     2401 ttggtggtat aattccggag ggtaaagggt acttctaccc gccgacatta ttaactgatg
     2461 ttaaacctgg aatgacagct tttgatgagg aactatttgg gcctgttatt gttattatta
     2521 ctgctaaaaa tgagagtgag gcaatttcgc tggccaatat gagtcagtat ggtttaggtg
     2581 ctgctgtatt tacaaaagat cttaataagg gagagcatat tgctacttat gaaattgaag
     2641 caggaacctg ttttgttaat gcttttgtag cctctgatcc aagacttcct tttgggggaa
     2701 taaagcgctc aggatatggt agagagttat caagagaagg tatattagag tttgtaaata
     2761 ccaaaacaat tgcgattagt gacccaaaac atggtaaaaa atagtaaatt catttttatt
     2821 tcaataaaaa aacaggagat gagttgtttt gaaaaggatc atttaattgc aaactatgct
     2881 atttcatccg ggaaaaatgg cgtgggtgaa aaaattaata gtggatgcac cccgagaggt
     2941 tggcataaga tccattctat acttggcatt gatctgccaa ttaatagtgt gatggttgcc
     3001 agagaatgga ctggtgagat ttattcagaa tcattggcaa ctcaatttcc agaaagggat
     3061 tggatattaa cccgaatttt acaattggat ggattagagc ctggtagaaa caagggcgga
     3121 gatgtagata ctttatatcg ttacatttat attcacggca caccagatac aactcaacta
     3181 ggtatacctg gctcacatgg atgcattcgc ctgaataatg atgcaattat tgagctggca
     3241 gcttgggcaa caaagaatac tttagtatac atacaatgat taataacaat taatgcttcg
     3301 gagcataagt gaacctgaag gggtaaatga atgtcctata aatttgaaga aggtaaagac
     3361 gcaatagtaa aaagcgttgt tgaaaaaatt aagcaaacaa tgactggcga acaaacagag
     3421 ttttgcgctg aattcgcgaa gcaattttat gggactgtag cccttgaaga tcttctagag
     3481 tgggaggttg atgatcttta tggagcagca gtcaatttct ggtctttgat ttgtgaacgc
     3541 gctccacacg aaacaaaaat caggatttat aaccctgatt atgaacgtca tggatggcaa
     3601 actacacata ctgttgtaga agttatctgc gaagatatgc cttttattgt agattctctg
     3661 aggatagtaa ttaatcgaat gggattaaca tctcatctta ctattcatat gggaggaatt
     3721 agggttaaaa gagacagcca taacagggtt actgaaattc ttccaagaaa tggtggagct
     3781 gctagagatg atgttttaca tgaggccccc atttttatag aaattgatcg acaaacagac
     3841 ccggctacct tatcggagct gcacaaaaat tttgaacgcg ctcttgagga caatcgagca
     3901 gtatttgaag attgggataa aatgcgtact aaagtacgag aaattattaa agagcttgat
     3961 acagtaccaa agacaattga tatcagcgaa attgaagaga cgaaagcctt tttaaattgg
     4021 atggaagatc accattttac ttttttagga ttgcgagatt atgatttggt taaaaaagga
     4081 aaagaaacca ttttacaacc tattcctgaa acaggattgg gcgttttacg ccaaagtctg
     4141 agcaaatcaa acgcaagaag tattacagca atggggccag aagcacaagg attaatttca
     4201 tcaccccgca ttcttgtaat gtccaaaaca aatactttag cctctgttca tagagatgct
     4261 tataccgact atattggtgt taagcgcttc aataaaaaag gagaggtgat tggcgaaagg
     4321 cgcattatag gactgtatac ttctgcagct tatcatacaa atcccaagca tattccgttt
     4381 ttgcgacata aagtcgcatt aattatgaaa aattctaatt taaatcccta tagccatgca
     4441 ggtaaagtat tattgaatat acttgaaact cttccaagag atgatttaat tcaaggcact
     4501 gaggatgagt tactggaaat agctatgggt attttctata tgcaagatag aaaacgtatc
     4561 cgcctgtttg ccagagcaga tgtttacaag cgatttattt catgcctggt atatgttccc
     4621 aaggatcggt ttaatactga attaagatat gccatgcaaa aaattttggc agacagtttt
     4681 aacgctgaag agattacctt ttctactcag ttttcggaat cggtattggc aagaattcat
     4741 tttatagtaa gggttaatcc caaaaattta cccgattttg atttaaaaga aatagagcaa
     4801 aaattgattg aagtggggcg gtcgtggatt gatgatttgc aacatcattt acatgaagtc
     4861 tatggcgaag aacaagccaa tgcactttac tctcggtata aaaatgcgtt tccaatatct
     4921 tattctgata ccttttcacc aagaacggct gtctatgata ttaagcatat tgaaatgcta
     4981 agccctgaaa atccacttgg tattaatttc tataagccat tggatgaatc tgagaagagc
     5041 tttaggttaa aggtttatca gcatgacacc actattcctt tgtctgatgt attacctatc
     5101 ctggaaaaac ttggtttaag agcaattagt gaacgtcctt atgttttaaa atttgaagat
     5161 ggtaaagtag catggattaa tgattttgcc atgcaataca acaaagaaag cgagtttaat
     5221 atcgacgaaa taaaagagtt atttcaaaat gcatttgcca gagtatggtt tggtgatgca
     5281 gaaaatgatg gctttaattt attggtatta gcggccggat taaattggag ggaagttgct
     5341 gtattaagaa cctatgcaaa atatttcagg caaatagggt ttacatttag ccaggattat
     5401 atggaaacag cactaaataa taatgtaagc attgccaaga aattagttag gttatttgaa
     5461 attcggtgca atccccatga ctccaaaagg agagaggata gatacgttgc acttgtcgct
     5521 gaaatcttat ctgatcttga taatgtagcc aatctggatg aagacagaat tataagacaa
     5581 tacgtgcatg ccatcggcgc cactcttcgt accaattttt atcaagtaga cgctcaaggt
     5641 aatcctaaga attatatttc tataaaattg tccagcaaac tcattcctgg tgtaccaaaa
     5701 ccatacccca tgtttgaaat ttttgtttac tcgccacgat tcgaaggggt gcatttacgc
     5761 tgtggtaaag tggctcgcgg aggtttacgt tggtcggaca gacgtgaaga tttccgaacc
     5821 gaaattcttg gcttaatgaa agctcaacag gtaaagaact ctgtaattgt ccccagtggc
     5881 gcgaaaggtg gttttgtacc caaacagata ccggctaatg ctactcgtga agaaattatg
     5941 gaggagggaa tcagctgtta taaactcttt ataagaggtt tgttggatat aacagacaat
     6001 tacaaagaag gactcattgt aaaaccccaa aatgttgttt tttatgatga agatgaccct
     6061 tatcttgtgg tcgctgcaga caaaggcact gcgacatttt ctgatattgc caattcaatt
     6121 tcccaggaat atgatttttg gcttggagat gcttttgcct caggtggctc tgttggttat
     6181 gatcataaaa aaatggggat tactgctaaa ggggcatggg aatctgttaa acgccatttc
     6241 tatgaattga acagagatat ccaaaacaat gatttcactg tggttggcat aggagatatg
     6301 gccggggatg tctttggaaa tggcatgttg ttgtccaggc atatcaaact cgtaggtgct
     6361 tttaatcatg ttcatatatt tgtagaccct aatcctgaag ctgaagctag ctttaaagag
     6421 cgtgaacgat tattcaatct tcccagatct agctggacag actatgataa aaaactcatt
     6481 tccaagggag gaggggtttt ttctcgtagt gcaaaatcta tcccagttag ccctgaaatg
     6541 cagaaagtat ttggaataaa acaaacttcg attgaaccta atgatctaat taaagcaata
     6601 ttaaaagccg atgtggattt actttggagt gctggaattg gaacctatgt caaatcaagt
     6661 actgaaagta ataccagcgt aggagatcga acaaacgatg caactcgtgt taatgccaat
     6721 cagttgagat gcaaagttat aggcgaaggt gggaatttgg gattaaccca gttagcacga
     6781 gtggagtata gtttacacgg cggcaaagtc tatacagatt tcatagataa ttctggtggg
     6841 gttaattgct cagacaaaga agttaatatt aagattttat tgaataatgt tgtatcagct
     6901 ggggatctga ctccaaaaca aagaaatgag cttttaagta atatgactga tgaagtagcc
     6961 aaattagtgc tcagagataa tttcctacaa acccgagcca ttagcttaac tgcttctcaa
     7021 gccttaagag ccattgagtt acatgcaaga tatattaacg aactagaaaa aacaggaaaa
     7081 attgacagaa ctttagagtt tttacctgat gaaaaagtcc taatggaaca caaactaatg
     7141 gggaaaggat taactcaacc tggaattgct gttttaatgt gctacagcaa aaccatacta
     7201 aaagagcaag ttcttgcttc tgaagttcct gaagaagatt atatgaatca aatacttatt
     7261 ggttcatttc ccaagcctct gcaagaacgt tttagtaaac aaatgcaaga tcatccctta
     7321 agaagggaga taattgctac ccgtttaagt aacattattg ttaatgaaat gggcttcacg
     7381 tatgtttaca ggttacaaga tgaaactggg gcctctgtcg cagcaattgt cagggcctat
     7441 atgattgcca gaagtgtctt gaatctggaa gcaatatgga aacaaattga ggaacttggt
     7501 acaaaaatca gcgcgcaaac tcaagttgat atgatgatgc tgtatgttcg actatccagg
     7561 agagtaacaa ggtggttttt acggacacat agaaggtcga tgagtataac gcaggcgata
     7621 gagttatatg caaaaggggt agatgaactg aaaaaatcca tgcccgccgt ttttggtgaa
     7681 acaggccgga ttcaatatga ggagcattac caagagcgga taaaagaagg gataccgcct
     7741 caacttgccc atgaattaac agtgactcga ggcttgtttg ccgcaacaga cattatagaa
     7801 attgcctatg aggaaaatat aaaaatacca aaagtagctg aaatatattt tggtattggc
     7861 gaatttcttg acatcgcttg gcttagaacc caaattattg ttcacacaac tgaaaatcat
     7921 tgggaatctt tatctcgaga ggcattacgc gatgatttgg attggcaaca acgccagtta
     7981 acagctgcca taatgggttt tgagcctaat aataaggatt tgcaagaacg attaacaaga
     8041 tggggagaga cacacgtttc tttaattgac aggtggaatt atattctgac agctctgaaa
     8101 tcaagtacag ctttgaacta tactatgttt ttagttgcca cccgagagtt gctggattta
     8161 acccaaacga cattacaatc caatttaaaa atggaagagt cattttaaca tgaaaggagt
     8221 taaatgaaat ggaggctaaa actgcttcag aatattcaac actgagcaaa ttatttcatt
     8281 ggatcattgc attggcagtt atagtcatgc taatagcagg tttttttctt gatgaaatac
     8341 ctgagcaatt tcagggggtt gcttatatgc ttcataaatc aacaggaatt actatccttt
     8401 tcttgatgat tttgaggttt atttggatcc atgctaaagg aaaacctgct ttaccagcgt
     8461 ctgttaaatc ttgggagaaa atattgtcaa ggtttgtgca atatggtttt tatgtgctct
     8521 tgcttattat gccattgagt ggttggatca tgtcggttgc ggcagacaga ataccaaatt
     8581 actttggctt gtttaaagcg ccacttccat ggattgaacc caataaatct ctagcagaat
     8641 caatggctga atcccatgaa attattgcgt ggattttaat cgccttcatc atactacatg
     8701 ttttaggtgc tcttaagcat cattttattg ataaagacaa tgtattgaag cgtatgctgc
     8761 ctggaaaaag caaatagccc tctgtttttg tttgaaaatt gagcctgaac tttaaagttc
     8821 aagctcaatt ttttcttcta attggattgg aaatttattg ccttccaaac agacagatga
     8881 aaagttctct tttaatatgc ttgcagcaac caaatatcga ttttcaccaa gcatggagta
     8941 atcaattaac tctccaacct ctatgtcttc atccgattta aataattttt gaccagaata
     9001 taattgatta tctgactgaa tcacaaattt ctttaattca tgcttaagag tagctctata
     9061 gtgagttctt gcgatgattt cctgaccttt ataacaccct ttatcaaaac tgacgtacgc
     9121 ggtttgatgt aagccaattc tatgtggtaa aaagagtccc ctagaattag gataaatatc
     9181 aatttgattg ttaaataatc taagggtatg ccaggttaat gaaccaagta attgattatt
     9241 ttcaataaaa ggcttgcaca aactatcata atagtctgaa tggattaaat agatatagaa
     9301 tcctttgcct aagtgataaa cacaaccatg actcgtacaa gataaagcat acaattcgct
     9361 ggacaaaggt agtaactgtg gtatttgatc attactatgc tgtaaataaa agcctaaaat
     9421 tttatagttg ttatttgaag taattttaac acgagaaagc agtgcaactt tgtttaatga
     9481 atgctgagtt acttcaatta aatcttgggg aagaactaat tttacacctt gccagttgat
     9541 aatatccaat aaggaaagaa ttctcccttt aaggttacat tgagctcctt gaatcattga
     9601 aacatctgat accaatcgta aatcacaagt taactgccct tgcagaaaat ctattgattt
     9661 ctctccaaga aaatcgatga cacctagata ggataagtca aaaagatagt ttttctgctt
     9721 atttaataat aattcagact ctatagggcg gaatgttatt aaagacctat tattcactaa
     9781 atgttctaat atagttttca tgtcaagatc aatgatgttg tttataatac agactagtgt
     9841 atcatttggc ctctcaaaat cagatatcat tattgaataa ttaggattat gttgtattta
     9901 tcgtgaagta acagaggaaa tccattgtgg acaatagaga aaaatcaagg ttattatgga
     9961 aatgcaggcg aggaatgcta gagcttgatc ttcttttaca gaaattcata gccaatgaga
    10021 ttgatcgact tacagaaaat caattgaaag catttgataa tctattaacc cacaatgacc
    10081 ctaatttata tgcctggtta atggggcatg aggaacccga aaaagagtta cttgaaattg
    10141 tatcattcat cagaaactgt gattaaacca ggtaaatcca aactatatct tcgattaatt
    10201 gtgattcttt atttgtgcac cattggatta gttttatatt cttctttacc tatgttgata
    10261 ggaggcgtct taatttttct tatcgttatt caatttaaaa atgatataac ttaccaaagt
    10321 gcttgccaaa aagttaaaga aatcaaattc ttgaaaaacc ttacgtggtc actggaaaca
    10381 atagacggta ctacccatca ttatgacaaa ctaaaaattc ttattcataa tatgctattt
    10441 caagttattc aattatcctc agataaaaaa aatcagatac taattctatt taatgatcaa
    10501 atacctaccg atcaattacg attattacac ctaaacagca acaaaaagta gtatataata
    10561 atacataatg attaaaacaa atccaaaata aataatgatt agactttatc ctattcgaat
    10621 gtaataacaa gcaaaggaat aatgaggaag ttatgcatat tgagacctct ttaagtgatg
    10681 aatctctaat tgaattagct caaaagggaa atgggctagc ttttgataaa ctgtttgagc
    10741 gttatcaggg caaaattaga caaatgattt atttttatac gcatgactgg acaaatgtca
    10801 gtgatttaac tcaggaagtt cttatcaaag tatttagaca tttatcttat tttaatgagc
    10861 gcagt
//
LOCUS       DATNSN010000113        10767 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.113, whole genome shotgun sequence.
ACCESSION   DATNSN010000113 DATNSN010000000
VERSION     DATNSN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.113"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            164..481
                     /locus_tag="VAH76_002267"
     CDS             164..481
                     /locus_tag="VAH76_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEO1386869.1"
                     /translation="MSRKLRIEEKLIPALSPIFLSVEDESMNHHVPQGAETHFKIIAV
                     SNHFNDLTRINRHKFVNQLLKEEFTLGLHALSIHLYTSDEWMTQQQSVLKSPACRDGY
                     KNK"
     gene            556..1977
                     /locus_tag="VAH76_002268"
     CDS             556..1977
                     /locus_tag="VAH76_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1386870.1"
                     /translation="MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIF
                     SAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTI
                     LSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
                     VPMGFIILLAFIWLIHGHPLAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVH
                     VRNVNNPQKNFPKAMFFSVILILFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQV
                     YDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPASLCHVNKHGMPAKI
                     LILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDME
                     RPFAIPGGKPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGILLML
                     LPAALIYLRKRYITKSKALNAPV"
     gene            complement(2107..3537)
                     /locus_tag="VAH76_002269"
     CDS             complement(2107..3537)
                     /locus_tag="VAH76_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEO1386871.1"
                     /translation="MKIFKFSSWLLLFVFTLDANAYYTSQGRIYDKSGNRVTINGVSW
                     SGFQDTNVFQGLQSNPFYNITMPQSKPRNGLMDLLVHPWSMADSGVNSSTAVEFKTVR
                     LPIQPGVLYDDSGEVDLNKWLSDKTQPEAGNGLFCKTWQSNGQSCEKAVSPKQAFWTV
                     LAEMKKHNINVMIDFHHRYGYGDGMRDGTVYSMAQYEKDLVLLAQEIKQRGLDNVIGI
                     DVFNEPYQLSWFKARNGQVSWITVIATAAKAVHQVNPDLLLFVEGPGGGNDDMDNPTI
                     CVPKANIKEDSGYAHWRDPGQCGNDQEVVAFKGNWGEDFKPLLDKNLAKQNIAQFDVQ
                     KFSNELQLAVPGIDTETLSWLLGDDNMGSNGHLVFSPHVYPKEVAGWETAPGDASNLR
                     FDWSWGFLYKAGYPIVLGEASWKSFEGKNFFTQALMPYLYQSGIGSNNLYFWAIGYLG
                     DTVSAINPNTGELNLDVQQTLKPYFN"
     gene            complement(3783..4535)
                     /gene="kdsB"
                     /locus_tag="VAH76_002270"
     CDS             complement(3783..4535)
                     /gene="kdsB"
                     /locus_tag="VAH76_002270"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEO1386872.1"
                     /translation="MSHNFHVIIPARYHSSRFPGKLLQEINGITVIERVYRQALLAEP
                     KSVIIATDHDEIADRAIQFGAEVVITSHTHQTGTDRIAEVVAKGSFAPDDVIVNVQGD
                     EPFIRPKLIQQVACSLTKTKAPVSTLCWPISSLEILNNPNVVKVVRTRDNHALYFSRS
                     AIPFHRDDKNAYSNTFRHIGLYAYRAAFLLEFVSWPPCTLEQIECLEQLRILWSGFSI
                     RVDEACEEPLQDINTKEDLILAQQYFLDTSNI"
     gene            complement(4532..4711)
                     /locus_tag="VAH76_002271"
     CDS             complement(4532..4711)
                     /locus_tag="VAH76_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019231979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEO1386873.1"
                     /translation="MDKKLLEILVCPLCKGKLLFKKQELICKFDRLAFPVRDDIPVML
                     EQEARLIPLEEKDKL"
     gene            complement(4704..5375)
                     /gene="tsaB"
                     /locus_tag="VAH76_002272"
     CDS             complement(4704..5375)
                     /gene="tsaB"
                     /locus_tag="VAH76_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO1386874.1"
                     /translation="MMKLLAIDTSTELASVAILIGDEIISREQDSQRIHAQLILPMID
                     ELIAQAGLGLNQLDGIIFGCGPGSFTGLRIACSIAKGLAYANDLPLVPVSSLAAIAWT
                     AREIKEDFNQPVLSVLDARMHEMYWSCFLEQQFLAQDRINAVKDIQLPANQSFILAGV
                     GIDLYWMDFPEQIKSQISEVLTVFPTASAMIRLAQKANIKAVSVAQAQPVYVRNQVTQ
                     GDSRG"
     gene            complement(5353..7311)
                     /locus_tag="VAH76_002273"
     CDS             complement(5353..7311)
                     /locus_tag="VAH76_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEO1386875.1"
                     /translation="MEEITTFAETCRRTLSEKGRLSTAIPGFVARAPQADLAVAIANA
                     IVQRSILVAEAGTGTGKTFAYLLPCLLSGKKALISTATKTLQDQLFQKDLPTLIRALG
                     LSVRIQNLKGRSNYICQYRVEKHAEEGQFQSPQCAHEILHVRDKLSQMQEGVRSELPE
                     INEDSSVWHYVTSTTDNCLGAECPNHETCFLVKARKRAMEADIVVINHHLFFADSQLK
                     KDGFSELLPGVDVVVFDEAHQLADIASNFNGERLGTRQFKDLVDDMLNEWPILDLANQ
                     PLKSISHKADKLVDQLLNALPTREERISWELVKRNKEFMEAWNTWLSLKDELIQCLES
                     KDAADIPGLKRCQERLLDLETVLLSFTEENNHKIRWLERFKHTLVFHATPYDVAESFS
                     QLLKDQSCAYIFTSATLTMASAFDSFCKPLGLDKVQTLLLSSPFDYQQQALLYLPRGI
                     PDPKDAHYYEALLEKALPVIEACGGRCFFLFTSHKAVKLVAQMLSNTLKYPLLIQGDE
                     AKPILLSRFRQLGNAVLLGTSTFWEGVDVKGEALSCVIIDKLPFANPVDPVIRGRMAY
                     LKKKGLSGFDELSLPNAVIALKQGVGRLIRDITDKGILMIGDPRLTGREYGRTILASL
                     PGLPKTRDEEKVLHFIKELALNDETVSD"
     gene            complement(7299..7634)
                     /locus_tag="VAH76_002274"
     CDS             complement(7299..7634)
                     /locus_tag="VAH76_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEO1386876.1"
                     /translation="MTFVVTESCIRCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVNAIVSEDDLTDEQQQFKELNAELSKTWPNITAKKDAPSDAKDWEEVKDKL
                     QYLQKEWKK"
     gene            7917..10709
                     /locus_tag="VAH76_002275"
     CDS             7917..10709
                     /locus_tag="VAH76_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1924 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386877.1"
                     /translation="MPLNLPPKSSKDIMPLVIAYNNAPEDNKIQKLFYLQKINYLLNK
                     TQLNDDLFDWINDAEEGGWLNELAKFSINPNASFFLKGMQFAKAITEEIKNKPEINSS
                     EINIYQLMQERDQLLKEVEFEKCATRYVEINFLLNELALNDKKTKEIVERQTEILRLV
                     APKIKAIKGESIDNLPVIPSYKTKELGNHVNNFNFKFTMSGWEAPFVFRVEDRHELGK
                     EQELHSYGVSKYFIEDYSVFMMRFKAEDGSTVYKPVILSQFANQNNLEEIAKQLKDGS
                     PKNIAPRIGYYFVQLTDFCLKLIETHNYHPDIKLNNFLVHNNRVLVSDRKTFTTNDNP
                     LASEILTSPLFAPDEFLKCLLFNKEGDPVGYNRNALWKRMNMPQFMAYQLGMALKQFL
                     ILTQLDELPDDFRNPDHSALSHFKTPSRQIINLSLLVQELTRLDPDKRMTIKQFQTLL
                     NFKNLPPDAFYQKVEEVFPSSQLGIAEDIDALNKVLNSDLKGEALLKQANPVFTKLSK
                     YDPKETRLTRLAEKLAIRCFNNMSKPYFQKLSGLVESALEKDANEIRQLLNPQSNREG
                     LLQKASPIFLKLFERQPEVPQLTDLAKQLAAQCFDKSSKNYFTRQLPLLIEEELLNQD
                     WEQAPWYRKALHWLTFGYFRVDNVTEISSLKIPEHTKGKEFQTYFSQLLFLPSEEIKN
                     MFTSKDSQTAGFLLGYIEKEREKREMETPSVQIPVDLKGEEFQMHFPQLEFLPSKDFE
                     SIGEKEGEHLECFIFANLQEILSHNNGDSSEKNSTSEPSDSESEIHLNTGNVDTIIIA
                     NEPNPTEAKPQKKDNDGAKQNNPAKASSPEGKVSTPEIDEHPKKKITDDTPKPKTKRS
                     VHFFDETKKSKPKVKENASPPVDETPPAKRKNVCRIDSVRSTLFRGDGSHRQKIKEQR
                     PRLSEIAWEPPKPNLQ"
BASE COUNT         3384 a         2290 c         1926 g         3167 t
ORIGIN      
        1 gtgaagtgaa tagacaaaat atttcataaa ataaactatt tgttattaaa taataataaa
       61 atgacaattt ttgtcatatt ctgacaattg tgtttaatta atgtgacatc tattcttcaa
      121 atgtatatac ttcttatttt gtaaaaacag ggtcgagcaa atcatgtcgc gtaaactccg
      181 catagaagaa aaactaatcc ccgcattatc tcccatcttt ttaagtgttg aagacgagtc
      241 catgaaccat catgttcctc aaggcgctga aacgcatttt aaaataatcg ccgtttcaaa
      301 tcattttaat gatctgactc gtattaatcg tcataaattc gttaatcagc ttcttaaaga
      361 agaatttact ctggggctgc atgcattaag catccattta tatacttctg atgaatggat
      421 gactcaacag caatcggtac tcaaatcccc tgcttgcaga gatggctata aaaacaaata
      481 attttaatga aaaaagttca cctccaagct tgatttattt tataatgtct atctttttat
      541 caaatagaaa aatcaatgaa atcatggtcc tctgaaaaaa tatccgttct tgctttagta
      601 ctgctcatta ccggagcaat tgacagcatt agaaatttgc caggcacagc actttttgga
      661 tctactttga tttttttctt tattttttca gcaattatct ttttgatccc ggttgcttta
      721 gtcgctgctg aactgtcctc aacctggtca gacgaggaag gtggtattta cagttgggtc
      781 aagcatgcct ttggtgaaaa cattgccttt ttcgccattt ggctgcaatg gataaatact
      841 ctggtatggt atccgactat tttgtctttt atagcaggca caatctctta tttaattaat
      901 ccagaacttg cacaaaacaa atactatctt atcagcgtta ttctgattat tttctggtca
      961 ttaaccttga taggcttatc agggctgcgt gcctctgcca cctttgccgg cttctgtgca
     1021 atagttggaa tgatagtgcc tatgggattt atcatccttc tggcttttat ttggttaatt
     1081 catggccacc ctcttgccat tgatcttagc ctctccaatt tgctaccgca atggaaaaac
     1141 acacaatcct gggtttctct aacagcgatt atgacttctt ttctcgggat ggagcttgcc
     1201 gcagttcacg tgcgcaatgt caataacccg cagaagaatt ttcccaaagc gatgtttttt
     1261 tcggtcattc tcatcctatt caccatgatt ttaggctcac tggccattgc tttcattctg
     1321 cctaaagaca aaatcagttt ggtagatggg gttatgaggg catttagtaa tttcttgcaa
     1381 gtttatgatt tgaattggct aatgcctgta ttaatactct tgttattgct gggtacctta
     1441 ggcagcatga tcaactggat tatttctcca gccaagggcc tgctgatggc agccaaacat
     1501 gattttctgc ccgcttcgct atgtcatgtg aataaacatg gcatgccagc aaaaattttg
     1561 atactgcaag ccattctggt gacagtatta tgtagcggct ttctcttatt ccctacagtg
     1621 aatgctattt attggttgtt tactgctctt agtacagagt tgtatatgat gatgtatgtg
     1681 atgatgttta ttgcagcctg gcatttgaag agtaaattct cagacatgga aagacctttt
     1741 gctattccag gaggcaaacc cggttattac ctgacatgcc tgttagggtt aataggttgt
     1801 actgtcactt tatgggtagg ttttattcct ccaacggaaa gcattaatat cggaggaaca
     1861 gcccattacc gtatggtctt ttcattaggc atcctcttaa tgctgctacc tgctgccttg
     1921 atctatttgc gtaaacgcta cattaccaaa tccaaagcgc ttaatgcacc tgtttaaatc
     1981 atcgatagga tggttgtaac ccgtggcttg tccatagggt cagtgtttta gcggagaact
     2041 cctggatccc gtggacaagc cacgggacgt cgaagatcag tacagtactg ggttatcaga
     2101 gatggattaa ttaaaataag gcttcaatgt ttgttgtaca tcgagattta attcgccagt
     2161 attcggattg atagcactaa ccgtatcacc taaataccct atcgcccaga aatataaatt
     2221 attggatccg atgccgcttt ggtaaagata aggcatcaat gcctgggtaa aaaagttttt
     2281 tccttcaaaa gatttccagg aggcttcccc taaaacaata ggatacccag ctttgtacag
     2341 gaaaccccaa ctccaatcga aacgcagatt acttgcatca ccaggggctg tttcccaacc
     2401 agccacttct tttgggtaaa catgaggtga gaaaaccaga tggccattac ttcccatatt
     2461 atcatcaccc aacagccaac tgagtgtttc tgtgtcaata cccggaacag ccagttgcaa
     2521 ttcattacta aatttttgta catcaaattg cgctatgttt tgtttagcca aattcttatc
     2581 cagaaggggt ttaaaatctt ccccccagtt ccctttgaat gctacaacct cctgatcatt
     2641 gccacactgc cctggatcgc gccagtgtgc atagcctgaa tcctctttaa tattggcttt
     2701 tggcacacaa attgttggat tatccatatc atcatttccg cctccaggtc cttcaacaaa
     2761 cagtaacaaa tcaggattga cctgatgcac tgccttggca gcagtagcta tcactgtaat
     2821 ccacgaaacc tgaccatttc tggctttaaa ccagctcaat tgatagggtt cattaaaaac
     2881 atcaatgcca atgacattgt ccaaaccacg ttgtttgatt tcctgagcaa gcagaactaa
     2941 atccttctca tattgagcca tgctataaac agtgccatcc ctcattccat cgccataacc
     3001 ataacgatgg tgaaaatcaa tcatcacatt gatattatgt tttttcattt cagcaagtac
     3061 cgtccagaaa gcctgcttcg gactcactgc tttttcacac gattggccat tggactgcca
     3121 ggtcttgcaa aaaagaccat taccggcttc aggttgtgtt ttgtcagaaa gccatttatt
     3181 cagatcgact tccccactgt catcataaag aacacctggt tgtataggca aacgtacggt
     3241 cttgaactct accgctgtac tgctgtttac accagaatcc gccatagacc aggggtgaac
     3301 caataaatcc attaatccat tccgaggttt ggattgaggc atggtgatat tataaaaagg
     3361 attgctctgc aatccctgaa aaacattagt gtcctgaaat cctgaccagg acactccatt
     3421 gatagtcacc ctattcccgc ttttatcata gattctacct tggctggtat aataagcatt
     3481 cgcatccagg gtgaatacaa acaacaaaag ccaactgcta aacttaaata ttttcatgcg
     3541 cttttcctca ttaaaataac cataaagtta ttgttaagtt ggttaaaagc tcattattta
     3601 accacaaaac cacaatattt gaacagtata atcattaatt tttcaaaaat attacaaaag
     3661 gatgtcattt cgatgtgcgg aaaaaagtca agatcgtcac tccattatga tgaaaattga
     3721 gtggcgacca tacggagcgg ataaaacatt ccctcataga aaaaacgata aattacaatg
     3781 agttatatat tggaagtatc aagaaaatat tgctgagcca aaatcaaatc ttcttttgta
     3841 ttaatatcct gtaaaggttc ttcacaggcc tcatcaaccc ggatagagaa acctgaccat
     3901 aggatgcgca attgctccaa acactctatt tgttccaagg tacagggcgg ccagctaacg
     3961 aattccagta aaaaggcagc acgataagca tataaaccga tatgcctgaa ggtattggaa
     4021 taagcgtttt tatcatctct gtgaaaaggt atcgcgcttc ttgaaaaata aagagcgtga
     4081 ttatctcgag tgcgtaccac tttaaccaca ttagggttat ttaatatttc aaggctacta
     4141 ataggccagc ataaagtaga cacaggcgcc tttgtcttcg ttaaagagca agcaacttgt
     4201 tgaattaatt tgggtcggat aaatggctca tcaccttgca cattgacaat gacgtcatca
     4261 ggagcaaagc tacccttggc gacaacctct gcaattcgat cagttccagt ttggtgcgta
     4321 tgtgaagtga ttacaacctc cgctccaaat tggattgcac ggtcagctat ctcatcatga
     4381 tcagtggcaa taatcaccga ctttggttct gccagaagtg cttgcctgta aactcgttca
     4441 atcacagtaa tgccgttaat ttcttgaagt aatttacctg gaaaacgact ggagtgatat
     4501 ctggctggta taatcacatg aaaattatgg ctcataattt atctttctct tccagaggaa
     4561 tcaaacgcgc ttcttgttcc agcataacag gaatatcatc acgaacagga aatgccagcc
     4621 tatcaaattt gcagatcagt tcctgttttt taaacagcaa ttttccttta cataaaggac
     4681 aaaccaatat ttccaataat tttttatcca cgcgaatctc cttgagtaac ctgattgcgc
     4741 acatacactg gttgtgcctg cgccacagaa actgccttga tatttgcttt ctgtgccaaa
     4801 cgtatcatgg cagacgctgt cggaaaaaca gttaatactt cactaatttg tgatttgatc
     4861 tgctctggaa aatccatcca gtacaaatca atcccaactc cggccaaaat aaaggattga
     4921 ttggctggca gctgaatatc ttttactgca ttaattctgt cctgggctaa gaattgctgc
     4981 tccaaaaagc aactccaata catttcatgc atacgagcat ccaaaacaga caatacaggc
     5041 tgattaaaat cctcttttat ttcccgagct gtccaggcaa ttgcggctaa actactcaca
     5101 ggaacaaggg gcaaatcatt ggcataagcc aacccttttg caatgctaca cgcgattctc
     5161 aatcctgtaa aacttccagg accacatcca aaaattattc catccaattg attaagaccc
     5221 aatccggctt gggctatcaa ttcatcaatc ataggtaaaa tcaattgggc atggattctt
     5281 tgactatcct gctccctgct tatgatttcg tcacctataa ggatggcaac actggccagt
     5341 tcagttgagg tatcaatcgc taacagtttc atcatttaaa gccaactctt taataaaatg
     5401 cagtactttt tcttcatccc gagttttcgg aagcccaggt aaacttgcca aaatggttcg
     5461 accatactct ctaccagtca aacgtggatc tcctatcatt aatatacctt tatcggtgat
     5521 gtcacgaatc aatcgcccta ctccctgttt taatgcaatg acggcgtttg gcaacgataa
     5581 ttcatcaaaa ccggaaagcc cttttttctt taaataagcc attctgccac gaattacagg
     5641 atcaacgggg ttagcaaagg gtaatttatc tataatcacg caagaaagcg cctccccttt
     5701 tacatcaaca ccttcccaaa aagtagaagt gcctaaaagc acagcattac ccaattgccg
     5761 gaatcgagaa aggagaatgg gttttgcctc atcaccttga atcaaaagcg gatattttaa
     5821 agtattgctc agcatttgag caaccagttt tactgcttta tgactggtaa aaaggaaaaa
     5881 gcaacggcca ccgcatgctt ctataactgg taaagccttt tccagcaaag cttcataata
     5941 atgagcatct ttaggatcgg gtattcctcg aggcaaataa agcagagcct gttgctgata
     6001 atcaaatggg ctggataata acaaagtttg tactttatcc aatcccagag gtttacaaaa
     6061 actatcaaaa gccgatgcca tagtcagtgt agctgatgta aaaatatagg cacatgattg
     6121 gtcttttaat aattgactaa atgattcagc aacatcatac ggggtggcat gaaaaacaag
     6181 agtatgttta aaacgttcta accagcgaat tttatgatta ttttcctcag tgaaagaaag
     6241 caagacagtt tccaaatcca aaaggcgctc ttgacatctt tttaacccgg gaatatcagc
     6301 agcatccttt gattccaagc attgtatcaa ctcatccttt aaagataacc aggtattcca
     6361 cgcctccata aactccttat ttcgtttaac caactcccaa ctgattcgtt cctcacgagt
     6421 cggcaaagca ttgagcaatt gatccaccag cttgtctgct ttatgactta tagactttag
     6481 aggttgatta gctaaatcca ggattggcca ctcattaagc atatcatcga ccaagtcttt
     6541 gaattgacga gttcccaggc gctccccatt gaaattagag gcgatgtctg caagctgatg
     6601 agcttcatca aacactacga catcaacgcc aggtaatagt tcgctaaaac catctttttt
     6661 taactgagaa tcagcaaaaa aaagatggtg attgataacg acaatatctg cctccatcgc
     6721 tcgcttacgc gctttcacta aaaaacaggt ttcatgattt ggacattctg caccaagaca
     6781 gttatcagta gtggatgtga cataatgcca tactgaggag tcttcattaa tttcaggtaa
     6841 ttccgagcga acaccttctt gcatttgaga caatttatct ctgacatgaa gtatttcgtg
     6901 agcgcattgc gggctttgaa attgtccttc ctcagcatgc ttctcaactc gatattgaca
     6961 aatataattg gaacgcccct ttaaattctg gattcgtacc gataaaccca aagctctaat
     7021 caaggttggc aaatcttttt gaaaaagttg atcttgaagc gttttggttg ctgttgagat
     7081 aagcgctttt ttaccactta acaaacaagg caataaataa gcaaaggtct tacctgttcc
     7141 tgttcctgcc tcagctacca aaatcgatcg ctgaacaata gcattggcta tagccactgc
     7201 caaatcggct tgtggtgctc ttgccacaaa gcctggaata gcagttgata gtcttccctt
     7261 ctcactgaga gttcgccgac aggtttcagc gaaagtggtt atttcttcca ctctttttgc
     7321 aaatattgaa gtttatcctt tacttcttcc caatccttgg catcagaggg agcgtccttt
     7381 ttagcagtaa tattaggcca ggttttggat aactcggcgt ttaattcttt gaattgctgt
     7441 tgttcgtctg ttaaatcgtc ttctgaaacg atagcattaa ctggacattc aggttcacaa
     7501 agggcacaat caatgcattc atcggggtga atcaccagga aattaggccc ctcataaaaa
     7561 caatctacag ggcatacttc tacacaatcg gtatacttgc atcgtataca actttccgtt
     7621 actacaaaag tcatagcaaa ccttaaaaat tatctttaag aaatgactca tattaaagca
     7681 taaaataccc tactgatgat agaggagcct gatattttta tacccctggg tcccattcat
     7741 tattcaaaac taacctgaaa tcacaaactc aaaaccaacg gcatcatatg gattcttact
     7801 gctcataaaa tgcacttgaa gtctcgctac aaatatggta attttgatca ttttaaatct
     7861 attttgttca atattttaat gcactgtctg tgtaattaga tatctcggag tacttcatgc
     7921 ctttgaattt accgcctaaa tcatctaaag atataatgcc tttggtcatt gcgtacaata
     7981 atgcacctga agataacaag attcaaaaat tattttattt acaaaaaatc aattacttac
     8041 tgaataaaac tcaactaaat gatgatttgt tcgattggat aaacgatgcg gaagaaggtg
     8101 gctggttaaa tgagctggct aaattttcaa ttaatcccaa tgcatccttt tttttaaaag
     8161 gaatgcagtt tgccaaagcc attactgaag aaataaaaaa taaacctgaa attaacagtt
     8221 ctgaaataaa catctaccag ctcatgcaag agcgtgacca actgttaaaa gaagtagaat
     8281 ttgaaaaatg cgctactcgc tatgttgaaa ttaattttct cctgaatgaa ttagccctta
     8341 acgataaaaa aacaaaagaa attgtagaaa gacaaacaga aatccttagg cttgtcgctc
     8401 ccaaaataaa agccattaag ggagaatcca tagacaattt gccagttata ccttcttata
     8461 aaaccaaaga acttggcaat catgtgaata attttaactt caaattcaca atgagcggat
     8521 gggaggctcc ttttgtattt cgtgtagaag acaggcatga gttaggaaag gaacaagaac
     8581 tacactccta tggagtttcg aagtatttta ttgaagatta ctctgttttt atgatgagat
     8641 ttaaagcgga agatggttct actgtttaca aaccagttat cctcagccaa ttcgccaatc
     8701 aaaataatct ggaagaaatt gccaaacagt taaaagatgg cagtccaaaa aatattgcac
     8761 cgcgaattgg gtattacttt gtccagttaa ccgatttttg tctaaagtta atagagactc
     8821 ataactatca tcccgacatt aagttaaata attttctggt tcacaataac agagtactgg
     8881 tcagcgatag aaaaacattc accacgaatg acaatccttt agctagtgaa attctcacca
     8941 gtcctttatt tgctcccgat gaatttttaa aatgtctact atttaacaaa gaaggcgacc
     9001 ctgtaggcta taatcgcaat gccctgtgga aacgcatgaa catgcctcaa ttcatggcct
     9061 atcaactggg aatggctcta aaacaattcc tgattttaac tcaattggat gaattaccag
     9121 atgattttag aaatccggat cacagcgctt tgtcgcattt caaaacacca tccagacaaa
     9181 tcatcaatct ttcccttctg gtccaggaat taacacgcct cgatcccgat aagcgaatga
     9241 ccattaaaca attccagacc ttgcttaact tcaaaaactt accgcctgat gccttttacc
     9301 agaaagtgga agaagttttc ccctcttccc aattaggtat tgcagaagat attgatgctc
     9361 ttaataaagt attgaacagc gatttaaaag gtgaagcgct tctgaaacaa gctaatcctg
     9421 ttttcactaa actatcgaaa tatgatccca aagagacccg attaactcgt ctagctgaaa
     9481 aattggcaat tcgatgtttt aacaacatgt caaaacctta ttttcagaaa ctatctggtt
     9541 tggttgaatc agccctagaa aaagatgcta atgaaatcag gcagctatta aatcctcaat
     9601 caaatagaga ggggctttta cagaaagcca gccccatttt tttgaaactc tttgaaagac
     9661 agcctgaggt accccaatta acagacttgg cgaaacaatt agcggctcaa tgttttgata
     9721 aatcttcaaa aaactatttt actcgtcaac tgcctttatt gattgaagag gaattgttaa
     9781 accaggattg ggaacaagct ccctggtata gaaaagcact gcattggtta acctttggat
     9841 acttcagagt cgataacgta acagaaatat catccttgaa aatacccgag catacaaaag
     9901 gtaaagaatt tcagacttat ttttcgcaac tcttattttt accctctgaa gaaataaaaa
     9961 atatgtttac ctccaaagac agccaaacag ctggatttct tctgggttat atcgaaaagg
    10021 aaagagaaaa aagagaaatg gaaactcctt cagtccaaat tcctgttgat ttaaaaggag
    10081 aagaatttca gatgcacttc ccgcagcttg aatttttacc ttccaaagat tttgaaagca
    10141 ttggtgaaaa agagggtgag catttggagt gttttatctt tgccaatctt caagaaatac
    10201 tctcgcataa taacggcgat tcatcagaaa aaaatagtac tagcgaaccc tctgatagcg
    10261 aaagtgaaat ccatttaaat acgggtaacg ttgatacaat tataattgca aacgaaccaa
    10321 atcccaccga agcgaaaccg cagaaaaaag acaatgatgg ggcaaaacaa aataatccag
    10381 ccaaggccag tagtcctgaa ggaaaagtat caactccaga gattgatgag catcctaaaa
    10441 agaagataac cgatgatacc cccaagccaa aaacaaaacg ttctgtacac ttctttgatg
    10501 agactaaaaa atccaaaccc aaggtgaaag aaaatgcctc tcctcctgtt gacgaaacgc
    10561 cccccgcgaa gagaaaaaat gtctgcagga tagatagcgt caggtccacc ctattcagag
    10621 gagatggcag ccataggcag aaaatcaaag aacaaagacc caggttatct gaaatagctt
    10681 gggagccgcc aaaacctaat ctacaataac aaacagaggc ttacttcttg aggctttctg
    10741 cagccaccct gattatacag ggtggct
//
LOCUS       DATNSN010000114        10750 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.114, whole genome shotgun sequence.
ACCESSION   DATNSN010000114 DATNSN010000000
VERSION     DATNSN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.114"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..96)
                     /locus_tag="VAH76_002276"
     CDS             complement(<1..96)
                     /locus_tag="VAH76_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO1386878.1"
                     /translation="MKITDEELKKIISKLSGSKADDIQDDTALIED"
     gene            complement(106..1329)
                     /locus_tag="VAH76_002277"
     CDS             complement(106..1329)
                     /locus_tag="VAH76_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEO1386879.1"
                     /translation="MHQEHPGQTDTPKIDPWLIFVILMLMALMQTTTDQYIPSLPAIT
                     TALNSTEASIQLTISMFMLGLSLSHIFYGPLSDKIGRKPPLMFGVGLSVLGSLVCFFA
                     PNVSVLILGRFLQGFGIGCCNSVGRSLVRDLFTDRLLAKIGSYVGVVSIFIMAASPTL
                     GGFIQEMAGWKANFMFLFVFGILVWLLAFCILPETNKHLNPEATKIKVMAKNYFTLLR
                     SKVFVGYTLCACFAFAGMVSYLTIAPFLFQNTMGLSPLEFGRLTFFIAGAICFSGIVN
                     SQLVMRKGISFMVCTGVLFMIVGGLSLLLITMLGWSQIYFIMISMTLFSAGAGFTFIN
                     AFAGAFHPFPQMAGTAGALYASMQDLSAALTSGFIAGIKGYGQYSLAMILLILGLSSL
                     VAWYYLGLQDDAISN"
     gene            1483..2685
                     /locus_tag="VAH76_002278"
     CDS             1483..2685
                     /locus_tag="VAH76_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEO1386880.1"
                     /translation="MDINLMVFASPLFLILIGIETWVAYYKKSGVYQLNDSVNNFSSG
                     ILEEIGALPIKGLIIYSYYYLYEHYAIFNVNTQSIFSWLLLWIGVDFFYYWYHRVSHR
                     NSFFWIGHSVHHQSEHFNLSVALRQGYFQTLTSWVFYLPLALIGFPTWMFVIVASVNT
                     IYQFWIHTESIRKMGWFEKIFNTPSHHRVHHGKNPQYIDKNYAGSLIIWDKLFGTFEP
                     EVAPADYGVTEPLDSWNPFYANIKVVKDILYYGKGLKSKWDVLRAFFMPPEWIIHRLQ
                     QENSPVSKKTAIKKNTTSPGIYMLTNTAFAVFLYTYLSLVFDPAMVTSWLMGALILLT
                     LYTLGRIADGHSNTLYLEISRATLILLILLLFKNTLLLSLGCSVLFYVVNYALCTTLP
                     YKRTASSF"
     gene            2838..5180
                     /locus_tag="VAH76_002279"
     CDS             2838..5180
                     /locus_tag="VAH76_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEO1386881.1"
                     /translation="MHTIANETPVQINHPFDPITNVLKWILLVTAILCFLILIWGTVK
                     TYQLAPPLPDRFVTSTGETVMTSQDIVEGKAGFQRADLMDYGSLYGMGSYFGEDYTAK
                     YLVRLGELVQGELAKIRFNKAYSELTESEQYVINKEMQSSLQHLDLIEKTSILTPALA
                     TSIRQLQTEISQSLLNHNFMAGYTQAYSLNPDTALKTANFLIYSSLTTIARRPNQNIS
                     YTNNWPYEPSVGNTPSSGTFYWTWISFCFVFMGFGAVLYIYHRYLSDVDHGPMEPIFS
                     GFKPLTQSQRKVGQYFLVAALFLLLQIAVGAIMAHYYAERESFYGINVNTWLPFNFLR
                     DVHIQTPIIWIGLSWISAGIFMAPIISGKEAKGQGFLVDLLFYVTLLVVGGAILGNYL
                     GIMGYIDKAWFWLGNQGLSYIQLGRLWQIGFCIGLFLWSFIVFRGMWPTWDGLKKATF
                     EFWTGRIKLENLFWASTMNVAVLYCFGMIPLTGIEKSFTITDFWRWWVVHLWVEQSFE
                     FFAACATAYLLMGTGLVSRKAAERTVYFESILIFLGGVIGTGHHWYWTGTPEMWIPLG
                     SMFSFIEVLPLVLLIIDAIEHRQLIKKQGTFTYNLAYLYILGAAFWNFVGAGVFGGGT
                     LNAPLINYYEHGTFLTLNHAHTALFGAFGLLALGLIYFCLRYAAGESKAWSDRLGVYA
                     FWLYNAGLFLWIVLNFFPIGWSQLMAVYEHGLSYARSLEFYNTTLLWQWLRFPGDVVF
                     ALGALLMAYDFIVKIGPFFPKFVRNRRFIAGHPKATDPSL"
     gene            complement(5371..7203)
                     /locus_tag="VAH76_002280"
     CDS             complement(5371..7203)
                     /locus_tag="VAH76_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1386882.1"
                     /translation="MSDLQQLPKTILRFSWHFIKKSPWLYALFFIAPVVMVLETNVIP
                     YALKMIIDGITNHKGGKESIFQDIAPALYLGGVAWFGVILVLRLHNWWQAYLVPQFQA
                     NIRMTLFGYLIQHSHHFFSNQLAGNLANKISDIPRSLEAIRKIVSCNVNTTFAAIVVS
                     IVLMLTINPWFAFILLSWIIIQLFISFYSAKEINKLARVNAEDKSILKGKVVDSLNNI
                     NTVKLFVQNEYEKKYVWKTQDKEVQSNKRLVLFINFFRLLVDVPVSIMLIAMIYSVIS
                     FWQRNLISTGDVVFIFATSFAIMNQMWSLSNSLCDLFVEIGIVKQALAILSQPIEVQD
                     IANAKELKVTHGEIRFENVTFRHRDGQELFTNESLVIPAKQKVGLVGYSGGGKTTFIN
                     LILRYFDVDSGRILIDNQDISEITQYSLRKSISMIPQDTNLFHRTLLENIQYGKEDAG
                     RDEVVTAASLANCMDFIQNLPQGFNTIVGERGTKLSGGQRQRIAIARAFLHHTPILIL
                     DEATSQLDSLTEECIQESLQPLCEGKTTIVVAHRLSTLLNMDRILVFDKGTIVEDGTH
                     QELIENNGLYKSMWDAQTSGFLPVEQEDGAELAKEFDRPYLAQV"
     gene            complement(7367..7753)
                     /locus_tag="VAH76_002281"
     CDS             complement(7367..7753)
                     /locus_tag="VAH76_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEO1386883.1"
                     /translation="MYTVIVCLFIAILLPYLAKVPVAYAMHKAGGYNNRYPREQQARL
                     QGFGARALAAHQNAFESLLIFATASLTALATNSVTLMTQYFAIAYIISRLFYHLFYLL
                     NWSSLRSTVWFLGVICCLSILWLSIP"
     gene            complement(8148..>10750)
                     /locus_tag="VAH76_002282"
     CDS             complement(8148..>10750)
                     /locus_tag="VAH76_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1386884.1"
                     /translation="LNLTLEQLKEAQFKCIKRVVCSGGGAKGVVYPGSYKAMEDTGVF
                     KGVEELSGASAGAITSTMLALGMPSAVFRDKLLTTNLKNLMGSRIGSLFGTNPPGVSA
                     ITRDGKPLEDFIRENIISSVKTNIASIEKIETIAASDQTLQDLLAKLKTKFPVITFGD
                     LALLNHHFPDKFKQLNMPAVEFPNGAIQVFNSTLTPDVEIALACRASASIPVILKPVT
                     ITINGVEKQFVDGGLYDNLPTDYFDLQEDGTFSKNTKPEQTLVFAFGEGLDNKTNQVF
                     QALYGSRWDEIFEDQLFESLIRQSMLLDQYFDPDVCQICPKKMAAQQKKSIELFLKKR
                     VSDKTLTTQEAHAIRLAMNKALNSLLLNTDENQPFWMAYREENDAEKRSMLLIGVIKE
                     KMKPVLYDAGIIEQLKRDTLVQFMGGLNTPYKNTDQKEIGYHKLRSEYSLRTVELRVG
                     GINTTSFDEATKVARIMDALGYLDTINHITNHNLHDPNIFNEEKFYVDTVTRFKSIYH
                     GVLAGSKRDIKRDKLTKCIRALEFDLRSQGKSQEQISRQVFQLIKDNVERNLKSPEAF
                     ALSRAVEYHNKVLTEEELFKETYEEGCKRCGHFSVSNITGEPIYGLSSLHESIKEKDM
                     FELYLNQPAGNTQTRMDKVFASLENLFVKHPNEQGIAEYNTSLKAFETALATFIKTHS
                     SVKDQQFHENGYRLFVYIKKLTAGKPNLMRPKNLAEITTVLSCATKTIQEIDDKDKTE
                     KNVSELAKLSQQVLGKSSVWKNIGIALLSLACAALVCIGILAAIPSSGTSLLMAAVGA
                     AGLGVTAAAGTMVASSAVIGGILFVEGSEKGLARSVSLFKTALGEIAKDDPEEFSTGL
                     TQNPFIRAID"
BASE COUNT         3213 a         2161 c         1974 g         3402 t
ORIGIN      
        1 atcttctatt agggccgtat catcttgaat gtcatcggct ttagagccag ataatttgct
       61 gataattttt ttcaattctt catcggtaat tttcattaat accccttaat ttgaaatagc
      121 gtcatcttgc aatccaagat aataccatgc cactaaagaa cttaaaccta aaatcagtaa
      181 tatcatagct aaagaatatt gaccataccc cttaattccc gcaataaaac cgctggtcag
      241 cgctgcactt aaatcctgca tgctggcata taaagcccca gccgttccag ccatttgcgg
      301 aaaaggatga aacgcacctg caaaggcatt aataaatgta aatccggcac cagcactaaa
      361 taatgtcatt gaaatcataa taaaatagat ttgtgaccaa cccagcatcg taattaataa
      421 caggcttaaa ccgccgacaa tcataaaaag aacaccagta caaaccataa aggatattcc
      481 tttacgcatg accagctgac tgttgacaat cccgctaaaa cagatggcgc ccgcaataaa
      541 aaaagttaaa cgtccaaatt ccaaggggct caatcccatg gtattttgaa ataaaaatgg
      601 cgcaatcgta agataagaaa ccattcccgc aaaagcaaag caagcacaca gtgtatatcc
      661 cacaaagacc ttgctacgta ataaggtaaa ataattcttc gccatcactt tgatttttgt
      721 agcttcagga tttaagtgtt tattcgtttc aggaagaata caaaatgcta aaagccaaac
      781 aaggattcca aaaacaaata aaaacataaa attggctttc cacccagcca tctcttgaat
      841 aaaccctcct aaagtgggtg aggctgccat aataaaaata ctgacaacac cgacataaga
      901 accaatttta gccagcaaac gatcggtgaa taaatctctc accaaagatc gccccactga
      961 attgcaacac ccgattccaa acccctgaag aaatcgaccc aagattaaca cactgacgtt
     1021 gggcgcaaaa aaacaaacca aactacccaa tacacttagc ccaacaccga acattaacgg
     1081 aggttttcgt cctattttat ctgacaaggg accataaaag atatgagata ggcttaaacc
     1141 cagcataaac atagaaatgg ttaactgaat agacgcttca gtgctgttca atgctgtggt
     1201 aatcgctgga agagaaggaa tgtattgatc ggttgtagtt tgcatcaaag ccatcaacat
     1261 taatatgaca aatattaacc acggatctat ttttggggta tctgtttggc ctggatgttc
     1321 ctgatgcatc aaatatcctt tagaatgatt tctaacatgg ctgataacaa tatcattatt
     1381 tacaagcaaa acaaaagtat tagaaaataa ggcataggcc tcaacgtctg caaaatagaa
     1441 aatacctatt gtaaagcgtc tattactgtt caaaggatat ttatggatat aaatttaatg
     1501 gtttttgcct cacctctttt tttgatcctc ataggaattg aaacttgggt agcctattat
     1561 aaaaaatccg gtgtctatca actgaatgat tcggtcaaca atttcagcag cggtatcctt
     1621 gaagaaattg gcgcactccc catcaagggc ctgattattt atagttacta ttatttatat
     1681 gagcattacg ctatttttaa tgtgaatact caatcgattt tttcctggtt gcttttatgg
     1741 atcggggttg attttttcta ttactggtac cacagagtaa gccacagaaa cagtttcttc
     1801 tggataggtc attcggtcca tcaccaaagt gagcacttta acttatctgt tgccttaaga
     1861 cagggttatt tccagacatt aacttcctgg gtgttttatt tgcccttggc tctgattggt
     1921 tttccaacct ggatgtttgt cattgtagct tctgtcaata ccatctatca attctggatt
     1981 catactgaat ccattcggaa aatgggttgg tttgaaaaaa tatttaatac tccatctcat
     2041 cacagagttc atcatggcaa aaaccctcaa tacatagata aaaattatgc gggaagcctg
     2101 attatctggg ataaactgtt tggcactttt gaaccagaag tagcgccggc tgattatggg
     2161 gtcaccgaac ccctggattc atggaaccct ttttacgcca acatcaaagt ggttaaagat
     2221 atcctgtatt atggtaaagg gctaaaaagc aaatgggatg tgttacgcgc gttctttatg
     2281 ccgccagaat ggatcattca tcgcttgcaa caagaaaact cccctgtctc aaaaaaaaca
     2341 gcgattaaaa aaaatacgac ctcacctggg atttatatgt taaccaatac tgcatttgca
     2401 gtgttcttat atacttacct ctctcttgtt tttgatcccg ccatggtcac atcctggctg
     2461 atgggagcat taattttatt gacactttat acccttggcc gaattgcaga tggccatagc
     2521 aatactcttt acttggaaat atcgcgcgca acattgatac ttttaatatt attgctattt
     2581 aaaaacactc tgttattgtc gctcggttgc agtgttttgt tttatgtcgt caattatgcc
     2641 ttatgcacta ctttgcctta taaaagaacc gcatcttctt tctgagaagc agacgctatt
     2701 caacaaggat taaccctgtg ggtactggag atgtatggtt ttctaagaat cagtcttgta
     2761 gctaacatga attgtttctt gtaaagatcc tctttataat caatgccgaa ttgaaccatt
     2821 ccgctaagga tagcctgatg catactattg caaatgaaac acctgttcaa ataaatcacc
     2881 catttgatcc aataaccaat gttttaaaat ggattttact agtcactgcc attctgtgtt
     2941 ttctcatcct catctggggc actgttaaaa cctatcaatt ggcacctcct ttaccagacc
     3001 gttttgtgac ctccacaggc gaaacggtga tgacatcaca ggacattgtg gaaggaaaag
     3061 caggttttca acgcgccgac ttaatggact atggcagctt atatggtatg ggttcctatt
     3121 ttggcgaaga ctatacagca aaatatctgg tgcgcttggg agagttggtc cagggagaat
     3181 tggctaaaat tcgttttaat aaagcctatt ccgaattaac agaaagtgaa caatacgtta
     3241 ttaataaaga aatgcagagc tcactgcaac accttgatct tattgaaaaa acaagcatcc
     3301 tgacaccggc acttgccacc tcaatacggc aattacagac tgaaatcagt caatcgttac
     3361 tgaatcataa ttttatggca ggttataccc aggcctatag cttaaatcct gatactgcgc
     3421 ttaaaactgc aaattttctt atttattcct ctttaacaac aatagctcgc cgcccaaatc
     3481 aaaacatatc ttataccaat aattggccat acgagccaag tgttggcaat accccttcat
     3541 caggaacgtt ttattggacc tggatttcat tttgctttgt gtttatggga tttggtgcag
     3601 ttctctacat ttatcatcgt tatttaagtg atgttgatca tggacccatg gaacctattt
     3661 tttcaggttt caaacccctg actcaaagcc aacgtaaagt aggtcaatat ttccttgtag
     3721 ccgctttgtt tcttttactt caaatcgcgg taggagccat catggctcat tattatgctg
     3781 agcgcgaaag cttctatggt attaatgtca atacgtggct gccttttaat ttccttcgag
     3841 acgtccatat ccaaactccc attatctgga ttgggctttc atggataagt gcaggcatat
     3901 ttatggcacc catcattagc ggtaaggaag ccaaaggaca agggttttta gttgaccttc
     3961 tgttttatgt caccttgctc gtagtgggtg gagccatcct tggcaattac ctggggatta
     4021 tggggtatat tgataaagcc tggttttggc taggtaatca aggcttgtct tacatacaat
     4081 taggaagact gtggcaaatc gggttttgca ttggactgtt tctttggagt tttattgttt
     4141 tccgcggtat gtggcctacg tgggatggat taaaaaaagc cacctttgag ttctggacag
     4201 gtcgcatcaa acttgaaaac ctgttttggg ccagcactat gaatgtcgct gttttatatt
     4261 gttttgggat gatcccctta acaggtattg aaaaatcgtt cactatcacg gatttctggc
     4321 gttggtgggt tgtgcatctg tgggttgaac aatcctttga gtttttcgcc gcctgtgcta
     4381 cagcttattt gctcatggga actggtttgg tttccagaaa agcagcggag agaacggtat
     4441 attttgaatc cattttaatt ttcttgggcg gagtaattgg tactggccat cattggtact
     4501 ggactggtac tcctgaaatg tggattccgc taggctccat gttttccttt atcgaagtct
     4561 tgcctctggt tcttcttatt attgatgcga ttgagcatcg acaattgatt aaaaagcagg
     4621 gaacgtttac ctacaatctc gcttatctct acatattggg agccgcgttc tggaattttg
     4681 ttggtgccgg tgtattcgga ggaggcacac tgaatgcgcc actgattaat tactatgagc
     4741 atggaacatt cttgacttta aatcatgccc atactgcttt atttggcgca tttgggcttc
     4801 ttgctctcgg gttaatctat ttttgcctga gatatgctgc tggtgaaagc aaggcatgga
     4861 gtgatagatt aggtgtttat gccttttggt tatataatgc agggctcttt ttatggattg
     4921 tcctgaattt tttcccaatt ggctggtcgc agttaatggc agtatatgaa catggactat
     4981 cttatgcccg aagtcttgaa ttctataaca ccaccttatt atggcaatgg cttcgttttc
     5041 caggggatgt cgtatttgcc ttgggggctc ttcttatggc ttatgatttt attgttaaga
     5101 ttgggccttt ttttccgaaa tttgtccgta atcgccgctt tatcgctggc catccaaaag
     5161 caacagaccc aagcttatga ccaatgatag caaagacaaa gccagtgtat ttttttgaaa
     5221 aattattttg agaaactgag tttagaagtg agtgtcgaca tccagttgat ggatgtcggc
     5281 ttgcaatcaa attcaaggta agagatcagc ctttacctgg aacgggcgta tttggttcat
     5341 tttgaacgac aattgctttt tttggttaaa ttacacttgc gccaaataag gccgatcaaa
     5401 ttctttagct agctcagccc catcttcttg ttccacaggc aagaagcctg aggtttgcgc
     5461 gtcccacatg gatttatata atccattgtt ttctatcaac tcttgatgtg tgccatcttc
     5521 tactattgtt cctttatcaa aaaccaggat tctatccatg ttaagaaggg ttgaaagtcg
     5581 atgcgctaca acgatagttg tttttccctc gcataatggt tgcaaggatt cttgaataca
     5641 ttcttcagtt aaagaatcca gttgtgaagt ggcttcatct aaaatcaata taggagtatg
     5701 atgcaaaaac gctcttgcta tagcgattct ttgtctttgc ccccctgaca gcttagtgcc
     5761 cctttcccct actatagtat taaaaccctg aggcaaattc tgaataaagt ccatgcaatt
     5821 tgctaaagaa gctgctgtta ctacttcatc cctgccagca tcctctttac catattgaat
     5881 gttttctaat aaagtacgat ggaataaatt ggtatcctga ggaatcatac tgattgattt
     5941 tcttaaagaa tattgcgtga tctcgctaat gtcttgatta tcaatcaata tccttccgct
     6001 atccacatca aaataacgga gaattagatt gataaatgtt gttttacctc ccccggaata
     6061 gcctacaagc ccaacttttt gtttagccgg gatgaccaac gattcattag taaataactc
     6121 ctgaccatct ctatggcgaa aggtcacatt ttcaaacctg atttcaccat gagtgacttt
     6181 taactctttt gcattggcta tatcttggac ttctataggt tgggataata tggcaagcgc
     6241 ttgcttgact atccctattt cgacaaacaa atcacaaagc gaattactta gactccacat
     6301 ttgattcata atggcaaatg atgttgcaaa tataaagacc acgtcgcctg tgctgattaa
     6361 gtttctttgc cagaaactga tgactgaata aatcatggca attaacataa tactgacagg
     6421 aacatcgact aaaaggcgaa aaaaattgat gaaaagaacc aggcgcttat tactttgaac
     6481 ctctttatct tgtgttttcc aaacgtattt tttttcatac tcattttgga caaataattt
     6541 cacggtattg atattattca aggaatcaac gacctttcct tttaaaatac ttttatcttc
     6601 cgcattcacc ctggcaagct tattgatctc tttagctgaa taaaaactga taaataattg
     6661 gattatgatc cagcttaaca aaatgaatgc aaaccaggga ttaatggtta acattaaaac
     6721 gattgaaact acaattgccg caaaagtagt gtttacatta caagatacga ttttacgaat
     6781 ggcttccaat gatcgaggta tatcacttat cttattagcc aggttccctg ctaattgatt
     6841 agaaaaaaaa tgatgggaat gctggattaa ataaccaaat agggtcatgc ggatattggc
     6901 ttgaaattga ggaacaaggt aggcttgcca ccaattatgc aagcgcaaca ccagaatcac
     6961 accaaaccag gcaactccac ccaaatacaa agccggtgcg atgtcttgaa atatgctttc
     7021 tttcccccct ttatggtttg ttatcccatc aataatcatt tttaaagcat agggaatcac
     7081 attcgtttca agcaccatca ccactggtgc tatgaaaaat aaagcataaa gccagggact
     7141 tttttttata aaatgccagg aaaatctcag gatagtctta ggcaattgtt gtaagtcaga
     7201 catattcaca tccttgtaaa aaatctggaa cttctttttt aagatatcaa agtcaaagac
     7261 aaagagtaag tggttataac caattagatg ataaattgat agatttttgc gccttaaatc
     7321 atcacccggt gaattttttg aataaagaca taccttgcca aaatttttaa ggaatactaa
     7381 gccataaaat agaaagacag catattactc ccaaaaacca tacagtggag cgcagggaag
     7441 accaattcag caaataaaac aaatggtaaa aaagccggga aataatatag gcaatagcga
     7501 aatattgggt cattaaagtg acagaattag tcgccaatgc agttaatgaa gctgttgcaa
     7561 atatcagtaa agactcaaaa gcattttgat gcgctgccag agctcttgcg ccaaaccctt
     7621 gcaagcgagc ttgttgctct ctgggataac gattattata gccacccgct ttatgcatgg
     7681 cataagctac gggaactttt gccagataag gtagcaatat agcgataaac aaacaaacaa
     7741 ttacagtata catagtttta gagctttcct gacttaacaa ggatattaac tagaatgttc
     7801 tctcagttca accaattaaa tcttatttca ctcagaacca gagtcgaatt ctattagctc
     7861 tcttgcataa cctctttaaa gctcaaaatt ataaattcca gcaattaaac agagcttacg
     7921 aaaaattcat aaaaccgata gattttgtag ctcattgatt agataatttt taagtagact
     7981 ctttttaatt tggtagactg tttgagcagt ctgatacgca atttttttca agtgtacgca
     8041 caagcaatgt gattacgttg aatacgctgt ttgcagcata ggcacttatt gacgtggttt
     8101 atatgatcct ttctccatca actcgttgag aaattttttc tatttgatta atctattgcc
     8161 ctaatgaatg ggttttgcgt aagtcctgta gagaattctt ctggatcatc tttagctatt
     8221 tcacctaaag cagttttaaa taaagacact gatcttgcta gccctttttc actaccttct
     8281 acaaagagga tcccgcctat tacagcagaa gaggctacca tagtccctgc tgcggctgtt
     8341 acccctaagc cagcagcacc aactgctgcc ataagcaagc tagtaccact tgaagggata
     8401 gcggctaaga taccaatgca cactaaagca gcacaggcta aacttaataa ggcaatgcct
     8461 atatttttcc atactgagct ttttcccaaa acttgttgag ataatttagc caattcagaa
     8521 acattttttt cagttttatc cttatcatct atttcctgaa tggttttagt tgcacaagac
     8581 aaaactgtag taatttcagc aagattttta ggcctcatta aattgggttt accggcagtt
     8641 aattttttta tgtaaacgaa tagcctgtat ccattttcat ggaattgttg gtcttttaca
     8701 gaagaatgcg ttttaataaa agtggctaat gccgtttcaa aagctttcaa acttgtattg
     8761 tattcggcaa tgccttgttc attgggatgt ttaacaaaca agttttccag agatgcaaaa
     8821 accttatcca tacgtgtctg ggtattgcca gctggttgat tgagatataa ctcaaacatg
     8881 tctttttctt ttatgctttc atggagagaa cttaatccgt aaatcggctc tcccgttata
     8941 ttcgatacag aaaaatgtcc acatcgctta catccttcct cataggtttc tttaaataac
     9001 tcttcttcag taagcacttt attgtggtat tccaccgcac gcgagagtgc aaaagcttct
     9061 ggtgatttta aatttctctc gacattatct tttatgagtt gaaaaacctg tctagagatt
     9121 tgctcttggg atttgccttg gctccttaaa tcaaattcca aagctctgat acacttcgtc
     9181 agtttgtcgc gtttaatatc cctcttggat cctgctaaga ctccatgata aatggattta
     9241 aatcgagtga cagtatcaac gtaaaatttt tcttcgttga aaatgttggg atcatgcaag
     9301 ttatgatttg taatatgatt aatcgtatca aggtaaccta atgcatccat aattctagct
     9361 actttggtgg cttcatcaaa actcgtggta ttaataccac cgactcgcaa ctctactgtg
     9421 cgcaaagagt attcagaacg caatttgtgg tagcctattt ctttctgatc tgtattttta
     9481 taaggtgtat ttaaaccacc cataaattgc actagagtat ctcgctttaa ttgttcgatt
     9541 atacctgcgt catagagtac aggtttcatt ttttctttga taacaccaat caacaacatg
     9601 gagcgttttt ccgcatcatt ttcctctcga taagccatcc aaaagggttg attctcatct
     9661 gtatttaaca aaagactgtt taatgctttg ttcattgcca gcctaatggc atgagcttct
     9721 tgagtggtca atgtcttatc actgactcgc tttttcaaga ataattcaat agactttttt
     9781 tgttgcgcgg ccattttttt tggacaaatt tgacacacat cgggatcaaa atactggtct
     9841 agtagcattg attgtctgat caatgattca aataattgat cttcaaatat ttcatcccat
     9901 cgtgagccgt atagagcttg aaacacttga ttggttttat tatctaaccc ctctccaaaa
     9961 gcaaaaacca atgtttgttc cggtttggtg ttttttgaaa aagttccatc ctcttgtaag
    10021 tcgaagtaat ccgttggtaa attgtcataa agtccaccat cgacaaattg cttctctaca
    10081 ccattaatgg taattgttac tggcttcagg ataactggta ttgatgctga ggcacgacat
    10141 gcaagtgcaa tttcaacgtc aggcgtcaaa gtactgttaa agacttgtat agccccattg
    10201 ggaaactcaa ctgctggcat atttaactgt ttaaatttat ctgggaaatg atgattcaga
    10261 agagctaaat caccaaaggt aataaccggg aattttgttt taagctttgc tagcaggtct
    10321 tgtagtgttt gatcgcttgc agcgattgtc tcaattttct ctatactagc tatatttgtt
    10381 ttaacagagc taataatatt ttcacgaata aaatcttcca atggcttacc atctcttgta
    10441 atagctgaaa cgccgggagg attggttcca aatagggaac cgatgcgact gcccatcaga
    10501 tttttgagat tcgtagtcaa taatttatcg cgaaatactg cagaaggcat tcctaatgcc
    10561 aacatggtgg aagttatcgc tcctgcggat gctcctgaga gctcttcaac tcctttaaaa
    10621 acgccagtat cctccatggc cttataagaa ccaggataaa caacaccttt ggccccacca
    10681 ccactacaaa caactcgctt aatacattta aactgtgctt ctttaagttg ctcaagtgtt
    10741 aaatttagct
//
LOCUS       DATNSN010000115        10640 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.115, whole genome shotgun sequence.
ACCESSION   DATNSN010000115 DATNSN010000000
VERSION     DATNSN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.115"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            38..628
                     /locus_tag="VAH76_002283"
     CDS             38..628
                     /locus_tag="VAH76_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386885.1"
                     /translation="MRMKMFSKTIPLTSQEAFEILCTRDYLEKISKIIFNFQQLFNVE
                     RSTLLSHHKLNPKISNNREFLQDLEARYDRLNHAVQNNEPYPFLYGDVCLLKEYLQVI
                     LGYYQEQLKEEQPVAKKNLRRIKGSHKFSTLMSDISKGEHPKLGKKDSEILIKYTINF
                     CAESTMWDDIKTISDLVIKPFLFDHKDEEGFSYCNP"
     gene            864..1625
                     /locus_tag="VAH76_002284"
     CDS             864..1625
                     /locus_tag="VAH76_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO1386886.1"
                     /translation="MKRIVFILLFISNVLVHSESLKVGVLQFAPPFSSISDKANHYYG
                     FVVALMNIICNRLQEKCEYIPIPKEGELKGLDKGVYHIVFSSTPITASLPANYRYSLP
                     YLPSDGQFLTLKNNSIDALNDINFKKVGFFKINFQQSPLLKKYETTNTLIEYTDPAEL
                     VNALTTKKIDAILINYQASRFIVNNLNPTEDDQLKLIGDKIPIGSGYAIIALKNQSAL
                     IDKINHILLEMEKDGSYLRIYNEYFGANPAYNLHH"
     gene            complement(1751..3079)
                     /locus_tag="VAH76_002285"
     CDS             complement(1751..3079)
                     /locus_tag="VAH76_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO1386887.1"
                     /translation="MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKM
                     NHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIES
                     AYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
                     NGNWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMS
                     NPRAYKINLPAMPFRPYFEEVNIGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPY
                     KPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKL
                     NRLSDHSIRPNQILLIPSAKSTPSLPFHEISKSKFLSQTPTKGKVYRVIHIVQANETY
                     NTLERLYKVTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKP"
     gene            3289..3741
                     /locus_tag="VAH76_002286"
     CDS             3289..3741
                     /locus_tag="VAH76_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386888.1"
                     /translation="MLMAPPAIIYDRLIFIRAVRVYFHMEMGWLVVCIKHQGMIRINI
                     FRRLEIMGTFFEWFPREVGLDKNPDAFRSDCYKYYWGEPPRWGGTVDACEVETDLYAM
                     VYNSIGLLSYLIPLVGGYLALHFARNANSKAFSEDNPEPGLEQSVLSP"
     gene            complement(3943..4713)
                     /gene="ceg19"
                     /locus_tag="VAH76_002287"
     CDS             complement(3943..4713)
                     /gene="ceg19"
                     /locus_tag="VAH76_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg19"
                     /protein_id="HEO1386889.1"
                     /translation="MFKKIITKLGIVMSKLRALEKEANELSLILDQALLSQSIEVKRR
                     ALSQFENSELIQNKDNLRVFIDPEHPIVSLIILATKTLPEKIENLQKKVRLLEIQLTA
                     ETEETLDESNLDSESVDYDNGIDEETVVPVTVTHQEHVSEPDTRSVAIARLREELKPY
                     VDSTQERTYEHYYGMAGTLFSFFGAAGYSKTDKLNAITKLFQNLEGDGAEPLNDTDRD
                     ILTTGVLGTTLKPLLEDEHIGNELKDLLSIKTDNVNTI"
     gene            complement(4875..5939)
                     /locus_tag="VAH76_002288"
     CDS             complement(4875..5939)
                     /locus_tag="VAH76_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HEO1386890.1"
                     /translation="MVNKICLSFALLISVPSILLAEDKLIFAVDIIRHGDRTPIVALP
                     TVNYQWQEGLGQLTAEGMQQEYKMGVAFRKKYIEESHLLPEHYEYGTIYVRSTDYART
                     LMSAQSLLMGLYPPGTGPTIPAGTSALPHAFQPIPVFSAPSKYDEVIIQQVDRKERKK
                     LMEQYVFSTREWQQKNNELKEKYPLWSRLTGINIDTLEDLETVGHTLYVHQIHNAPMP
                     EGLASNDIETIINSAEWAFMAQEKPQQIANVYSSKLMTNIADYLNSGSMKKSKLKYVL
                     LSAHDTTIASVLSFLGAPLEKSPPYASNVNFSLYDNGANYYTVKITYNGNPVLIPACG
                     GSVCELQQLINLVHDSKNSV"
     gene            6208..7323
                     /locus_tag="VAH76_002289"
     CDS             6208..7323
                     /locus_tag="VAH76_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO1386891.1"
                     /translation="MSRYVCKWKLIYTFLIFIVFNNCPLAFARPSLTKQLHTILKDNL
                     ADSLTPGAVLLISSPDLGTISVAAGLADKENDISMNITDSFRLASMSKTFLAVTVLKL
                     VADKQLNLDDHIADLLPDSIDIERIPNGDEVTVQQLLQMRSGIPNYTDYDSYSDLIDT
                     MVDKEWTPEECIKLVYDQKPDFKPGTAYEYSNTNYLLLQLIIENITEESYAESIKKYI
                     LKPLRLKNTYIEKQEFDDDHHYLSTHGYTLEDGKVVDVTDHDDGFGLADGGIISTAED
                     INIFVQALLKDKILLSPNYLTMMLTFPDDYGFGIGREEINGEIAWSHNGASSGYQGQY
                     YYFPDRQLTVVILTNYFDSDVIEDIASQTLSAIDNSE"
     gene            7415..7888
                     /locus_tag="VAH76_002290"
     CDS             7415..7888
                     /locus_tag="VAH76_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386892.1"
                     /translation="MTIYKFEISTMQDGNIQISAKHRLTGEIHGYVQLASSARLESRN
                     LLEINQLYVDNNARGHGLGSELASRAIKYALKMDKGIHMMIQPGSDVFWKKYFNSRFD
                     GKRITLFGGEALNTNLADGKVFEISKESLVANYKFPQDRHLRDRDMEKICAKLSH"
     gene            complement(7998..10346)
                     /locus_tag="VAH76_002291"
     CDS             complement(7998..10346)
                     /locus_tag="VAH76_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386893.1"
                     /translation="MRYSLLLLCTLTAIMTCVSANTSLKPSIAQPATCVNSLFTATGN
                     QHWKSIVLKLTNNCNQAVDFQNSTVSFQTAEALNTSFWGDFSPLSYPDNALNISSQPQ
                     SGGNYLATFNLHFPSYPGANSKLPVGSSISIKYGATTDSHIEGTANVYLSTTVESGSI
                     QLINAATKPTNVSQGYALVHVTMNGQSISDVQLPWQTSKTLSGLALGNYAISAETVTD
                     SNGNLYLGQANPSMVSVIASETTSSTINYARVQETGKIKIHVQNLPGELNNYNENPTV
                     LLTQSQSGNSRSQLAVWGNTTTVAELKEGSSYQFSTPAIQYNDYNCWPTFTPFSLVAS
                     ATNVPTTNLTYQCIQVAKDTVTLSVSGAPASLTSLKIILTPNDGSQTVEQTVDLANGV
                     GSSAITLTDGVIYTLSSEGVPGYTVQFSPQPLTATANATVNIALSPVTTEKGRIIGYI
                     PGWKTPPTAQELANAGYTHVMIAFGVFSTNTPGVIVPAFETITKEYIQSLHQAGIKVI
                     LSLGGALTSIPNTTVDFHQVLVASSSPEAFKQTFINSLKGLISQYGFDGFDIDIEHGI
                     NAGGSFSQPQGDIAVLASIINTMYSQNPSLLITLTPQVANIAATSGFDQTWGNYASLT
                     MQTHQSLAWVGIQLYNTGCAFGIDQVCYGPTPTDTPDFSVAMATDLLENWPATVNGRP
                     TGFQPYISYLRPSQVVIGYPSPNANGGSDGSPVTPTTTIKRAIQCLKTAIASNTSCGA
                     YAPPRAYGNIGGVFNWEVTYDKNNQFKFAKELKNCVINGVCE"
BASE COUNT         3371 a         2007 c         2004 g         3258 t
ORIGIN      
        1 gattcaaatt aaatctaaat caaccagaga tcacgtcatg cgtatgaaaa tgttttctaa
       61 aacaattcca ttaaccagcc aggaagcctt tgaaatatta tgtacgagag attatcttga
      121 aaaaatttca aagattatct ttaattttca acaattgttt aatgttgaaa gaagtactct
      181 tctctctcat cacaagctca accccaaaat aagtaacaat cgagaattcc ttcaagatct
      241 ggaagctcgt tatgatcgcc tcaatcatgc agtacaaaat aatgaacctt accctttttt
      301 atacggcgat gtatgtttat tgaaagaata cctccaagtc attttaggct actaccagga
      361 gcaacttaaa gaagagcaac cggtagcgaa aaaaaattta agaaggatca aaggaagcca
      421 taaattttca actctaatgt ctgatatctc aaaaggggag catcctaagc ttggcaaaaa
      481 ggattcagaa attttaatca agtacaccat taatttctgt gctgaaagca ctatgtggga
      541 tgatataaaa acaattagtg accttgtaat aaaaccattt ttatttgatc acaaagacga
      601 agaaggtttt tcttattgca atccataggc ttgagaatag atgagagggc agtgacctgg
      661 atttgacgac atctctaaat cggatactgc ccaaaccctt ccaagcccta ccttgaaaag
      721 ccttttaatt gcgcagctaa caatatttta tttcactaaa attgactccg aaaaaccgat
      781 ccaaaatgaa aactattgcc ctataattaa aaaagattac ttctcttatt aaaaatggac
      841 cagattaaga agagacccaa tttatgaaac ggatagtatt tatactgttg tttataagca
      901 atgttctagt tcatagtgaa tcactcaagg tgggtgtctt acaatttgca cctcctttct
      961 catctatatc agacaaagcg aatcactatt atggtttcgt tgtcgccctc atgaatataa
     1021 tatgcaaccg tttgcaagaa aaatgcgaat acatccctat tcctaaagaa ggcgagttaa
     1081 aaggcctgga taaaggtgtt tatcatattg tttttagctc aaccccaatc acggcatcat
     1141 tacctgccaa ttatcggtac agcttgccct acttacccag tgacggtcaa tttctgacat
     1201 taaaaaacaa ctcgattgat gccttgaacg atatcaattt taaaaaagtt ggtttcttta
     1261 agattaattt tcagcaatct cctcttttaa aaaaatacga aaccaccaat acgcttatag
     1321 agtacacaga tccggcagaa ctagttaatg cactaacaac caaaaaaatt gatgcgattt
     1381 taatcaatta tcaagcgtca cgattcatag taaataatct caaccccact gaagacgatc
     1441 aattaaaatt gattggcgat aaaataccga taggaagcgg atatgcgatc atagccctta
     1501 aaaaccaatc ggcattgatt gataaaatta atcacatttt gctggaaatg gaaaaagatg
     1561 gttcctacct ccgtatctac aacgaatatt ttggtgcaaa cccggcatat aatcttcatc
     1621 attagttggt tagtatgaaa atcgccacat atgagaaatt taaccaaatt tattaataag
     1681 ttatcgaata gttaagcgtg aaattttgac cgaacacggg ttatgtttag aatgcaggat
     1741 tgccggaaac ttaaggtttt ttgaaagtcc tgttccattc aagattgctt gactttttcc
     1801 aaataattaa ctgtcgccct tttaccaacg cttctcccga gccaatatga ttccatttca
     1861 taatatcctg agctgtaact ttataaagtc gctcaagggt attatacgtt tcattggctt
     1921 ggacaatatg tatgaccctg tatactttac cttttgttgg tgtctggctt aagaattttg
     1981 actttgaaat ttcgtggaaa ggcaatgatg gagtactttt ggcactcgga attaacaaga
     2041 tctgattggg tcttatcgag tgatctgaca gacgatttaa tttttttatt aatgaaacag
     2101 tcgtatgata ttttctggca atactttgta aactatcact cggaaggacg agatgttttc
     2161 tccagctcac tcgtttatca acaggtgaat gggataaatt gaaataaaat tgagtcactt
     2221 ttgctatagg gataagcaag gtgaagggtt tatagggtgc ggtagcccat ctattataac
     2281 ccggatttaa tttggttaat tcactaaccg aaattcctgc taaactagcc gcatgattca
     2341 agtcaatttg actgccaata ttcacctctt caaaataagg tcgaaatggc attgcaggaa
     2401 gatttatttt ataagcccga gggttgctca tgatttcagc cagagctaaa aatcgaggaa
     2461 cataaatttg agtttcttta ggtacatcca gattccaaaa actgacacta cctccattag
     2521 tattatttgc ttttacagct ctgcctatgg ctccttcgcc agcatcgtaa gctgcgatgg
     2581 ataatatcca gtttccgtta aacactttat ttaaatgcat cagatgagtc agggctgcat
     2641 cagttgaatg attaatgctt cgtcttccat caaaccacca atcctgaatt aaacctaatt
     2701 gattccccgt caccggcatt atttgccata aacctgcagc accaactttt gagtaagcaa
     2761 aaggatcata agcgctttct atcataggga gtaatgccag ctcaccgggt aaatttcttc
     2821 tttgtatttc tcgagttaca tgataaagat aaggctcagc ctgtttacag gccttagtca
     2881 caaaccctgg atgatccagt agccagcgaa tttgatcttg aacttctggg cgataggctt
     2941 catgattcat cttgaattca ctgcgcaaaa catcccatac atcaggtgtt tcaaatgact
     3001 tagcatgagt actgaagata aaaaaaataa ggcaactgaa tagcagctgc atttttttat
     3061 cgatgaatgt cttaatcaaa ttactattcc tgctctataa agcttctctg gagcccgttt
     3121 ataatgtatt gtttctcttc gcctgaaccg ggcctgagcg cgtcttataa aatatactcc
     3181 tcttagtatc ttgtataaag cacttcctgc aatataaggc catgataatt taacaccctg
     3241 cctatttaac gttttttatt aatattgctc taacatttgt ttcgatatat gctgatggct
     3301 cctccagcaa ttatttatga cagactgatt tttatcagag cggttagggt ttattttcat
     3361 atggaaatgg gatggctggt tgtgtgtatc aagcatcaag gaatgataag aattaatata
     3421 ttcaggagat tggaaattat gggcactttt tttgaatggt ttcccagaga agtaggttta
     3481 gataagaacc cggatgcgtt taggtcagat tgctataaat actattgggg agagccccct
     3541 cgatggggcg gcaccgttga cgcatgtgaa gttgaaactg atttatacgc tatggtatat
     3601 aattccatag gacttttgag ctacttgata cctttggtag gtggatattt agcattacat
     3661 tttgcacgaa atgcaaactc caaagcattt tccgaggata atccagaacc tggccttgag
     3721 cagtctgttc ttagccctta aataagctga actatttcta aaaatcaata atttaaaaat
     3781 ccagataaaa agatacgctt ttaaatcgaa tcccgttaaa agattttcat atatcaggaa
     3841 tttttcctag gaagccggat cattgatccg gcatcagatg gattgatgag ataagataag
     3901 tgttttagat gaacaggaaa actcctgttc aaatatgagt ttttaaattg tattcacatt
     3961 atcggttttt atcgatagca aatctttcaa ttcattacca atatgctcat cttccaacaa
     4021 cggcttcaga gtagtgccaa gaactcctgt agtcagtata tctctatccg tatcattgag
     4081 tggctcagcg ccatccccct caagattttg gaacaatttg gtgatagcgt ttagtttatc
     4141 agttttactg taacctgccg ctccaaaaaa actgaacagt gtccctgcca ttccataata
     4201 gtgttcatat gtcctttctt gagtagaatc gacataaggt ttcaactctt ctcttaatcg
     4261 tgcaatggcc acagacctgg tgtcaggctc actgacatgc tcttgatgag taacggtgac
     4321 aggaacaaca gtctcttcat caataccgtt atcgtaatca acactttcag aatctaaatt
     4381 ggattcatca agggtttcct cagtttcagc agtcaattgg atctcgagca aacgtacctt
     4441 tttttgcaga ttttctattt tttcaggtaa agtctttgta gcaagtatga ttaagctcac
     4501 aatgggatgc tcagggtcaa taaaaacccg caaattatct ttgttttgta ttaattctga
     4561 attttcaaat tgagataatg ctcttctttt aacctctatt gactgactca acagggcctg
     4621 atcaagaatg agagacaatt cattcgcttc tttttccaat gctcttagtt tagacataac
     4681 tattcccaat ttagtgataa tttttttaaa cagtgattaa ttataaaaca aaaacattaa
     4741 gataatctta acttatgaaa aatgggctat tttcccgaat gcctcaaacc agctgctgtt
     4801 gctctcttgc aacaacagat agtttgaggt gcttagactt actcacctga gccaaatacc
     4861 tccaggatga gtgatcacac tgaattttta gaatcatgca ccaaatttat aagttgttgt
     4921 aactcacata ctgaaccacc gcaagcagga ataagtaccg gattaccatt ataggtaatt
     4981 tttactgtgt aataatttgc tccattatca tagagagaaa aattaacatt tgaagcataa
     5041 ggtggggact tttccaaagg agcacctaaa aaacttaata cactggctat tgtagtatca
     5101 tgagcagata gcaaaacata tttcagttta gattttttca tgcttccaga attcaaataa
     5161 tcagctatgt tagtcatcag ctttgaacta taaacattcg ctatttgttg aggtttttct
     5221 tgtgccataa aagcccactc agcagaattg ataattgttt caatatcgtt agaagctaat
     5281 ccttccggca taggagcatt atggatttga tgaacatata aggtatgacc aactgtttcc
     5341 agatcctcca gagtatcaat attaatgcca gtcagacgac tccataatgg gtatttctct
     5401 ttcaattcat tatttttttg ttgccactcc ctggtagaaa aaacatattg ctccattagc
     5461 tttttccgtt ccttacgatc tacctgctga atgataacct cgtcgtattt agacggtgcg
     5521 ctaaatacag gaataggttg aaaagcatga ggtaaagcgg aggtccctgc aggaattgtt
     5581 ggtccagttc caggtggata tagtcccatt aacaatgact gggcgctcat cagggtacgt
     5641 gcataatccg tagagcggac atagatggtt ccgtattcat agtgttcagg taacaaatgc
     5701 gactcttcaa tatatttttt gcgaaatgcc acacccattt tgtattcttg ttgcatacct
     5761 tctgccgtaa gctggccaag cccctcttgc cattggtaat ttacagtagg caaagcaaca
     5821 atcggcgttc tgtcgccatg acgaataatg tccacagcaa aaataagttt gtcttctgct
     5881 aataaaatgg agggtacgct aattaatagt gcgaaactca ggcaaatctt gttgaccatg
     5941 tattcatact ccttgagtta aagtaaacca tgaggcttcg taaaaaattc ttgttgtatc
     6001 ttgccaccgt tggttaatct cggtttcgca ctatgacgaa gacacaaaca aaaatcaaga
     6061 catcggcttt tgtattcctt gattcatttt gaacctggag gagcaatcta ttttatacag
     6121 gaagtaaaga aattatcctg aaatcgacta gaattaagat atagctcatt tggtgggtga
     6181 gaaaaccctt gccacattgg agttgcaatg agccgttatg tatgcaagtg gaaattgatt
     6241 tatacgtttt taatttttat tgtctttaat aattgcccac ttgcctttgc gagaccttcc
     6301 ttaaccaagc aattacatac catattaaaa gacaatctcg cagactcatt aacccctgga
     6361 gcagtattac tgatatcaag tcctgatttg ggcacaattt ccgtcgcggc agggttggca
     6421 gataaggaaa atgatatttc aatgaatatt accgacagtt ttcgtcttgc aagcatgagt
     6481 aaaacgtttt tggctgtaac tgtcttaaaa ctggtagcag ataaacagtt aaatcttgat
     6541 gatcatattg cagacttatt gccagatagt atagatattg aacgcatacc gaatggagat
     6601 gaggtaacag tgcaacaatt actgcaaatg cgtagcggca ttcctaatta tacggattat
     6661 gattcttaca gtgatctcat tgatactatg gtagacaagg aatggacacc agaagaatgc
     6721 attaaactag tctacgatca aaaacctgac tttaagccag gaacagctta tgagtacagc
     6781 aatactaatt atttgttgct gcaattaatt atcgaaaaca taaccgaaga atcctatgcc
     6841 gaatcaatta agaaatacat cctgaagcct cttcgtttaa aaaatacata tatagaaaag
     6901 caagaatttg atgacgacca tcattattta tcaacacatg gctatacctt agaagatgga
     6961 aaagtggttg acgtaacaga ccatgatgat ggttttggct tggctgatgg tggaataatt
     7021 agtactgctg aagatatcaa tatttttgtc caagcgttgt taaaagataa aatactttta
     7081 tctccaaact atttaacaat gatgcttacc tttccagatg attacggttt tggaataggg
     7141 cgcgaagaaa ttaatggtga aatagcctgg tcccataatg gggcaagctc aggataccaa
     7201 ggccagtact actattttcc ggaccggcaa ttgactgttg tcattttaac caactatttt
     7261 gacagtgatg tcattgaaga catagcatct cagactctca gtgcgatcga caattctgag
     7321 taactacttg gctgcactca actaatggaa atccgtgaaa tagagtatat ggtatactaa
     7381 gtgaaaatta tctgtccttt tatcggagca aaatatgacc atttacaaat ttgaaatctc
     7441 aactatgcaa gacggcaata tacagatatc agcaaagcac aggctaacag gagagataca
     7501 tggctatgtt caacttgcct catcagccag gttagaaagc agaaatttgc tagaaataaa
     7561 ccagctttat gttgacaata atgccagagg acatggattg gggagtgaac tcgcctcgag
     7621 agccattaaa tatgctctta aaatggataa aggcattcat atgatgattc aaccaggctc
     7681 ggatgtgttt tggaaaaaat atttcaattc tcgttttgat ggaaaacgca ttaccttatt
     7741 tggtggagaa gctttaaata ctaatttagc tgatggaaaa gtatttgaaa tttctaaaga
     7801 aagtttggtt gccaattaca aatttcctca agatagacac ctgcgtgatc gcgatatgga
     7861 aaaaatctgc gcaaagctct ctcattgagc ggatgattat acaaaattaa aatatattgg
     7921 atcaaacaga taatacatga taaataatat ttcaagattt aagtgtcagg ctcaaagagc
     7981 ctgacctgac aaattgatta ctcacaaaca ccattaataa cacaattttt caattctttt
     8041 gcaaatttga attgattgtt tttatcataa gttacttccc agttaaatac accaccgata
     8101 ttcccataag ctcttggcgg agcataagca ccacaactgg tattactggc aattgctgtc
     8161 ttaaggcact gaatagcccg tttgattgtg gttgtcggag taaccggtga gccgtcactg
     8221 ccaccattag catttggaga gggataacca atgacaactt gggaaggtct taaatagcta
     8281 atataaggtt gaaatcctgt aggacgtcca ttgaccgttg ctggccaatt ctccaataaa
     8341 tcggtagcca tagctactga aaaatcaggg gtatcagttg gtgtaggacc atagcatact
     8401 tgatcaattc cgaaagcaca tcctgtattg taaagctgga tacctaccca agctaaagac
     8461 tgatgggttt gcatagttaa agaggcataa ttcccccagg tttggtcaaa cccgcttgtt
     8521 gcagcaatat tagccacttg aggtgtcaga gtaattagca gagaaggatt ttggctgtac
     8581 atcgtattga taatgcttgc taagacagca atgtcacctt gtggttgaga aaaggaaccg
     8641 ccagcgttaa taccatgctc aatatctata tcaaacccat caaaaccata ttgagaaatt
     8701 aaccccttta aagaattgat aaatgtttgt ttaaatgcct ctggtgaaga agaagctact
     8761 aaaacctggt gaaaatctac tgttgtattg ggaatactgg ttaacgcacc acctaaagaa
     8821 agaatcactt taatcccggc ttgatgaaga gactgaatat attctttggt tattgtttca
     8881 aaagcaggaa caataacacc cggagtattg gtactaaata cgccgaaagc aatcatgaca
     8941 tgagtataac ccgcgttagc caactcctga gcggtgggtg gagttttcca tcccggtatg
     9001 taaccaataa tacgcccttt ttcagtggtt accggagata aagcaatgtt tactgtcgca
     9061 tttgctgttg ctgttaaagg ttggggcgaa aattgtactg tatagccggg aacaccttca
     9121 cttgaaagtg tataaataac cccgtcagtt aaagtaatgg cagatgaacc aacaccattg
     9181 gccaaatcta ctgtctgttc tactgtttgt gaaccatcgt tgggagttaa aataattttc
     9241 aatgaggtca gggaggctgg agcaccactt acactaagtg tcacagtatc tttagcaacc
     9301 tggatacatt gataagtcaa attggtagtt ggaacatttg tagcacttgc caccaaggaa
     9361 aacggcgtga aagtgggcca gcaattgtaa tcgttatatt gaattgcagg agttgaaaat
     9421 tgataactac tcccctcttt caactcagca acagttgttg tgttccccca aacggcaagc
     9481 tgtgaacgtg aattcccaga ttgactctgg gtaagcagaa cagttggatt ttcattgtag
     9541 ttgtttaatt cgccaggaag attctgcaca tggatcttga tcttgcctgt ctcttgtacc
     9601 cgagcatagt taatagtcga gctcgttgtt tcgcttgcaa tcacactcac catgctggga
     9661 ttggcttgcc ccagatagag attgccatta ctgtcagtaa ccgtttcagc agaaatggca
     9721 taattgccta aagcaagacc ggaaagcgtc tttgaggttt gccagggtaa ttgcacatca
     9781 ctgattgatt gaccattcat ggtgacatgc actagcgcat acccttgtga cacgtttgtt
     9841 ggttttgtgg ccgcattaat taattggatg cttcctgatt caaccgttgt gcttaaataa
     9901 acattagctg ttccttcgat atgactatca gtggttgcgc catatttaat tgaaatagaa
     9961 cttccgacag gtaacttact attagcacca ggatagctgg gaaaatgcag gttgaaagta
    10021 gctagatagt ttccgccaga ttgaggttga gaagaaatat ttaaagcatt atccgggtaa
    10081 gataaaggag aaaaatcgcc ccaaaaggag gtgtttaacg cctccgctgt ttgaaaggag
    10141 acggttgaat tttgaaaatc gactgcctga ttgcaattat tggttaattt taatacgatt
    10201 gatttccagt gctgatttcc cgttgctgta aaaagagagt ttacacaggt tgcaggttgg
    10261 gcaattgagg gttttaaaga ggtattggca ctgacacatg tcataatcgc agttaaagta
    10321 catagcagta ataatgaata tcgcatcaat tcctcctcat ttgtgattgg aaaaaaatca
    10381 atctatcatc attttttctt atttaacaat aagctatatg aaaattgtcg gtttttttac
    10441 aattagcttt ttgtcttaaa tgaaataacc aaaccactca ccaattgagg tggttgattc
    10501 acagatttgc tttgccattt cacaaaatag taggttaatg aattaaaagc aaagacttgt
    10561 attctttatt taaacaatcc aacccaaagc ccaccataga atgcttcggt tagttttaaa
    10621 tacaactaaa aacaaaaatt
//
LOCUS       DATNSN010000116        10602 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.116, whole genome shotgun sequence.
ACCESSION   DATNSN010000116 DATNSN010000000
VERSION     DATNSN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.116"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1644)
                     /locus_tag="VAH76_002292"
     CDS             complement(<1..1644)
                     /locus_tag="VAH76_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO1386894.1"
                     /translation="MMNESLTEKRNELSLSFAALGVVFGDIGTSPLYAFGQVIKYFPI
                     NDHNIYGILSLIFWSLIIIVSIKYLVIVFRADNDGEGGIIALAGVIRQKIKQPGGWLL
                     FITLVGIGLIIGDGMLTPAISILSAVEGLESLSPNLAKYVLPVTLIILFFLFKMQSIG
                     TGKIGVYFAPVMLVWFITIGILGFLQIIQNPKVLMAINPYYAINFFMIHKYMALFILG
                     GVFLVMTGGEALFADLGHFGKKAIRTGWFVVALPALLLCYFGQGALVLMHTEDIKYPF
                     FSLSPDWFLPVMIILATLATIIASQAIISAAFSILKQASLLNLIPRLKIVFTSKFEKG
                     EVYLPLINFILALGTCSLVVIFKSSSNLADAYGIAVNLDMLITTVLVGIIAYRCWSWH
                     AFKILIFLLILIIELAFFAGNIPKLLTGGWIPILIAFLGFVVMYTWHCGFEKLRELHH
                     RDALMDAFIIDELNQNKISRQPGMGLYIIDPYDCEGESLLHHLRLNRIFFENMIFVSI
                     KIENKPYIPIEDKFELIKKAEGFYLIFIHYGFTENINLPN"
     gene            2049..3575
                     /locus_tag="VAH76_002293"
     CDS             2049..3575
                     /locus_tag="VAH76_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1386895.1"
                     /translation="MIMATTIPDIEAITFTDWFLRIIDTPGIPIDEIEISVIEGFKRT
                     LTDAQFQHLTSLIHLISFIKHRNFSNPTLALRIYIDENTSPLSRTALVEAIRMLPPEN
                     NKTYELVCRLAQKNKLEQYTNITKVPPLHIYQGDGVLERYDDWILLFELFDLTQITRG
                     DLIPFLADLLSSKNFDATELRALSKFIKTLDKLGIVSQNFMEKISPHLRNRKNIEQYE
                     SFFLKLQVNGLLTEKILEAIYPLLHQLDALEHFFCLYRQELLHDNTSSLLSDTVQSFC
                     NMSIPSKVSYDEPVSTNTPLHLAVIEGNLEHLVRTLAFANRNLLIKCSCENTALLLAC
                     KLADKKAARLILAKMQELGCDVNQRDHHGMTALHWANFYHFNELIKELENAGANPKLR
                     AGNGKTCEYFYQHQFTMQDLKLNGKEIIDGEFKLGDSALTDIAFHMDKIALNLKLTTP
                     SEIAELYSGDGMAQIRSSNRFNLFFRLFRLKLINWLDKQHDFESKPIDDISELTLSKK
                     "
     gene            complement(3699..6764)
                     /locus_tag="VAH76_002294"
     CDS             complement(3699..6764)
                     /locus_tag="VAH76_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386896.1"
                     /translation="MAYTLTLLGTDTTFTPKPDTGYEHGETLSYVSTLVDRPGEPQLT
                     DNITHYRNSNIAVVDGPDTMGKAVGDRIARGVASILEAISRGETDISIMAHSRGAVEA
                     ILVAHELERIQENFKQGKGSADLKNSKCPLTKTAMEAQKDTYESLNLEGIANNINNVK
                     LSIFNIDPVPGGDFLGAPVGWKDERFFRVPKIVKEYEQCVYENERTRCFKAIVPKCDS
                     SDTKFNLFSLPGHHGTGSGNLKDQQQQAVPGGETTTEHAQQLMIIKLIDFLNRNGVAI
                     KPGQNSDEGQVNNLSFDDLIGPLFDEDGKIKKEKLKEKYLTLYQDIIKNKAAYEHFNN
                     TSYPVLGREQSIQKVFGSYRDDRIILHQAYKDAFLSDVLPHLPGGHFVNYDHARLTIN
                     KELALEDGLPLNEVIDKAVERLNEIIRHSSQVTKDVAEAQSLSESCINDKYAPVIQSE
                     EGFNILVNSLEGLISEVRNIYFHDKHKSMSDDERNALFNSVKGAIDSFKSADLEDNQL
                     ATDVRGKIDETIKKILEGKITRLNDDAEILSQKLGENLSEVVKSKFDILRQKPALVDV
                     HNDRLKEFDDFKNQLISLLESKPPQIKESRELLEEKLKSFEKTDPPDVVNEDMTKFFG
                     ELLEEVIPDYDVNKLMEDANQLYDEFDSFKESFSSFKELNDSLNYAQWQTELEEKQRQ
                     LIDQTARYIQKNNLNLEKDIDPLFKDNKSPLYEQIKLVASNYGLENPDFVKLKRQNSE
                     LSCKLDEQIEKIKELQQIKERHDHDKIELEKLNQELQVLRDSGSEKEKEVEQIRQEIA
                     ALRTRIQSQDRQQDALEKQIKNLNSEIAWLSTDKQQLTREREFQDNRINDLNELVNRL
                     NTDEEQECTAVVRTLKKLTADHLDYLTAKKGNTQNDTIKEQIEITKNLQGILCNTKEY
                     PLPSERIKAFTNQLKKDNEKLSAQHDPDPAWKRFAKSCLITIGVICSGIIPGILALAT
                     YNHFKDKSSPQSFTAKYKNELSKIKQQSDISKHQTEDETQNLTTTGLAR"
     gene            7088..7576
                     /locus_tag="VAH76_002295"
     CDS             7088..7576
                     /locus_tag="VAH76_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO1386897.1"
                     /translation="MKIRAIFFIIAIFLPFTLFASDATCPLSNGINVLTKKRILNICK
                     NGTVVKTFKVALGYKGVGKKKAGDNKTPIGLYGLAHPRKSNQFKVFIPILYPTKKQLA
                     AGYTGRDVGIHGPTQSPGVFSWLNNLPGSTRGCVAVGKNNYIEYVANWVKANPGAKVL
                     II"
     gene            complement(7609..7899)
                     /locus_tag="VAH76_002296"
     CDS             complement(7609..7899)
                     /locus_tag="VAH76_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386898.1"
                     /translation="MCNEDGSILKILKEIKAIIKEIDPQEEQSISDALRLIRNVIKDG
                     ARQERPLVAEVLKIFNATGSLNFRQIRNSLEHLPGLNEFSLSSRRPLSGHST"
     gene            8095..8298
                     /locus_tag="VAH76_002297"
     CDS             8095..8298
                     /locus_tag="VAH76_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386899.1"
                     /translation="MPLYVSDCQSNFSVINNGHHLLHQKQAGIEKSKITQTAAQAANN
                     TNFAKVTIESEYDVDSEKKVTQV"
     gene            8384..8773
                     /locus_tag="VAH76_002298"
     CDS             8384..8773
                     /locus_tag="VAH76_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386900.1"
                     /translation="MDIVKAQKNMKVKVNVLRIPANEREANIVAVYSILINKDLMGDM
                     DHIPNVIWQIKSIIENINLDDDDDIARSICLIKEKIENSNENYTNKNIMDFLNAFSKN
                     SDLTFRQIRQELAQSNSEMKKILDTYD"
     gene            complement(9021..10556)
                     /locus_tag="VAH76_002299"
     CDS             complement(9021..10556)
                     /locus_tag="VAH76_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1386901.1"
                     /translation="MQKAKAKIQNINEIMFSDRVMLLNKNLLVSIPANLLCALIIYIG
                     LDKTIDEEILFVWFMVGVTVFALHGGLFLFNYYRPLPSKYLLKWLISVTAIYGVLWGI
                     AGSVLIPQSDLLNQMIVIIIIIGIASGGLHILQPSFLASLFFFTLTLIPLSVWFFQQN
                     TLNYWFLGTALLIYFCFLSIISWMGYGLLNKNFKLLYENLDLINKLYAINANLEESEL
                     RFRSAFNSAAIGMAIVSLEGMWLKVNQSLCQIVGYSEEELLKTNFQSITYPEDLELDL
                     GYVRQLLEGDIRFYHMEKRYIRKNGSVIWILLSASLIRDPENKPLYFISQIQNIDAQK
                     RAAQELQFIAYHDILTGLGNRKLLELSFDQALAHAKRHQKQIAVMFMDLDYFKNVNDK
                     LGHDIGDLLLIEIGKRLKTILRSTDLIVRYGGDEFIIALTELSNVNQVIEIANKILIA
                     ISKPITIKRYGISITGSIGISIYPYDGYDLDTLIRKADEALYRVKIGGKNNFRLFNTA
                     LHK"
BASE COUNT         3464 a         2012 c         1711 g         3415 t
ORIGIN      
        1 atttggtaaa ttaatatttt cagtaaaccc ataatggata aaaattaaat aaaacccttc
       61 cgctttcttt ataagctcaa atttatcttc aatagggata taaggtttat tctcaatttt
      121 gatacttaca aaaatcatat tctcaaaaaa aatacggtta agacgaagat gatgaagaag
      181 actttcccct tcgcaatcat aagggtcgat aatatataac cccatcccgg gttgtcttga
      241 aattttgttt tgatttaact catcaataat aaaggcatcc atcagagcat ctctatgatg
      301 caattcccgt agtttctcaa agccacagtg ccaggtatac atcaccacaa atccaagaaa
      361 cgcgattaaa atagggatcc agcctccggt taacaacttg ggtatattgc ctgcaaaaaa
      421 tgcaagttca ataattaata tcagaagaaa aatcaatatt ttgaacgcat gccaactcca
      481 acaacgataa gcaatgatcc caactaaaac agtggtgata agcatatcta aattaacggc
      541 aatgccgtat gcatcagcaa gatttgatga cgatttaaaa atcacaacaa gtgaacatgt
      601 acctaaagcc aaaataaaat tgataagtgg caaatagact tcacccttct caaatttgga
      661 cgtgaagaca atcttaaggc gtggaatcag attaagcagc gatgcctgtt ttaaaattga
      721 aaatgccgct gaaataattg cctgtgatgc aataatcgtt gcgagcgtag ccaaaataat
      781 catcacaggg agaaaccagt ctggcgataa actaaaaaag ggatatttga tatcttctgt
      841 atgcatcaat acaagcgctc cctgaccaaa atagcataaa agcaatgcag gaagcgccac
      901 gacaaaccag cctgttcgaa tggccttttt gccaaaatgc cctaaatcag cgaataaggc
      961 ttcaccacca gtcattacta aaaatacccc acccaatatg aagagagcca tgtatttatg
     1021 tatcataaaa aaattaatcg cataataagg atttattgcc atcagtactt tgggattctg
     1081 tataatttgt aaaaaaccca aaattcctat ggtaataaac caaacaagca taacaggagc
     1141 gaaataaaca ccaattttcc ctgtacctat gctctgcatc ttaaagagga aaaataaaat
     1201 aattaatgtc actggtagaa catatttggc caaattggga gacaaagatt ccagaccttc
     1261 gacagcactt aatatagata ttgctggagt taacattcca tcaccaatga taagcccaat
     1321 accaaccaaa gtaatgaata aaagccagcc tcctggctgt tttattttct gccttatcac
     1381 ccctgctaaa gctataatgc cgccttcgcc atcattatcc gcacgaaaaa cgatcaccag
     1441 atacttaata ctgactataa tgatcagcga ccaaaaaatg agcgataaaa taccataaat
     1501 attatggtca ttgattggaa aatactttat tacctggcca aatgcataaa gagggcttgt
     1561 ccctatatca ccaaaaacaa cccccaaggc tgcgaatgat aagctgagct cattcctctt
     1621 ttctgttaaa gactcattca tcatccggga ctactcccaa tatcatagta aatcctttat
     1681 gaattattat tttgagtata gtactatctg atatcaaaaa tgaattgaca tttagtatct
     1741 gaaggccaat gaataacaga tttgcctgca tatccgctct ctatgactca attccttaaa
     1801 atgagcgatt aacaacttca aacagcttcg gttataaatg caaaccatat gaaattggta
     1861 tgcgttataa gctttaatat ggccatattt tgcatagttc aaaaagaaga aaataacttt
     1921 ctaaaatttt ttaatactaa atgctctctg tacatgacaa aaagacctgt catgtacaga
     1981 actaaatgct attttttttg ttacaaaaat gaatctattg gttataataa catcatttgg
     2041 tttgggaaat gattatggcc acaacaattc cagatattga agcaatcact tttaccgact
     2101 ggttcctgag aataattgac actccaggta ttcccatcga tgaaattgaa attagtgtaa
     2161 tcgagggttt caagagaaca ttgacagatg ctcaatttca acacttaacc tcattaatcc
     2221 atttaattag tttcattaag cacaggaatt tttccaatcc taccctggca ctccgaatat
     2281 atattgatga aaataccagc cctttatcta gaactgcctt ggttgaagca atcaggatgc
     2341 ttccgcctga aaataataaa acctatgagt tagtatgccg tttggctcag aaaaataaac
     2401 tggagcagta tactaacata acgaaagtgc ctcctctgca tatttaccag ggggatggcg
     2461 tgcttgagcg atacgatgac tggattctgc tttttgaact gtttgatcta acccaaataa
     2521 cccgtggtga tttaattcca tttcttgctg atttattaag cagcaaaaat tttgacgcca
     2581 ctgagcttag agctctttcc aaatttatta aaaccctgga caaattaggt atagtaagcc
     2641 aaaattttat ggaaaaaatc tctcctcatt tgcgtaacag aaaaaatata gagcaatacg
     2701 agtcattctt ccttaaattg caagtcaatg gcttgttaac cgaaaaaatt ctggaagcaa
     2761 tttacccgct acttcatcaa ttagatgccc ttgaacattt tttttgtctc tatcgtcaag
     2821 agctattgca tgataatacc tcttcattac tatcggatac agtacaatcc ttttgtaata
     2881 tgagcatccc aagtaaagta agctatgatg aaccggtatc gacaaacacc ccattacatc
     2941 tcgctgttat tgagggcaac ctggagcatc tcgtacgaac cttagccttt gccaatcgca
     3001 atttactaat aaaatgctcg tgtgaaaata ccgctctttt gttagcttgc aagcttgcag
     3061 ataaaaaagc ggctcgattg attttggcaa aaatgcaaga attaggctgt gatgtcaatc
     3121 agagagatca tcatggtatg accgccttgc actgggctaa tttttatcat tttaacgaac
     3181 taatcaagga actggaaaat gctggggcaa accccaaact acgagcaggg aacgggaaaa
     3241 cttgcgaata tttctatcag catcaattca ctatgcaaga tttaaagtta aatggcaaag
     3301 aaatcattga tggtgaattc aagttaggtg atagtgcgct aacagatatt gctttccata
     3361 tggataaaat agccttaaat ttaaaattaa ctaccccatc cgagattgca gagctgtatt
     3421 caggagatgg aatggcacaa attcgttcga gcaatcgatt caatttattt ttcaggttat
     3481 ttaggctcaa attgattaac tggttagaca aacagcatga cttcgaatct aaaccaattg
     3541 acgatatatc ggaactcacc ctttcaaaaa aataacattg gctaaactcc atttacttta
     3601 aatgcaatgt atttaaatat gcctcagggg taactgaggc atacagactt tatgaatatt
     3661 tgagtaatgc tctctcttaa acaaaaattt cttaatcttc acctagccaa tccagttgtt
     3721 gtcaaattct gggtttcatc ttccgtttga tgtttggaga tatcagattg ttgtttaatt
     3781 ttgcttaatt cattcttgta tttagccgta aaagattgtg gggatgattt atctttaaag
     3841 tgattatacg tggcaagggc aagaatacct ggtataatcc cactacaaat tactccaatg
     3901 gtaatcaggc agcttttcgc aaatcgtttc caggctggat ctggatcatg ctgcgcgctt
     3961 aatttttcat tatctttttt aagttgattg gtaaacgctt taattcgctc actcggtaat
     4021 ggatattctt tcgtgttaca taaaatccct tgcaaatttt ttgtaatttc aatttgttct
     4081 tttatagtat cattttgtgt atttcctttt tttgcagtta aataatcgag atgatctgcg
     4141 gtcaatttct tcaacgtacg aacaacagca gtacactcct gttcttcatc tgtatttaat
     4201 ctattcacta gttcatttaa atcattgatt cgattgtctt gaaattcacg ctctctagtt
     4261 aactgctgtt tatcagtgct taaccaagca atttcactgt taagattctt aatttgcttt
     4321 tctaaagcat cttgttgtct atcttgagat tgaattctag ttcttaaagc cgcaatttct
     4381 tgtcttattt gttcaacttc tttttctttc tctgagcctg aatccctcaa aacctggagc
     4441 tcttggttta atttctctaa ttctatttta tcgtgatcat gtctttcttt tatttgttgc
     4501 aactctttta ttttttcaat ttgttcatct aatttacatg acaactcact attctgccgc
     4561 tttaacttca cgaaatctgg attttccaaa ccataattgc tggcaacaag tttgatttgt
     4621 tcatacagtg gagacttgtt atctttaaaa agaggatcaa tgtccttttc aaggttgaga
     4681 ttgttctttt gtatatagcg agcggtttga tcaatcaatt gtctttgttt ttcctccaat
     4741 tcggtttgcc attgagcata gtttaatgaa tcattaagtt ctttaaatga agagaatgac
     4801 tctttaaagc tgtcaaattc atcatacagt tgattggcat cctccattaa tttgtttaca
     4861 tcataatctg gaataacctc ttctaataac tctccaaaaa attttgtcat atcttcattc
     4921 actacatctg gaggatctgt tttttcgaat gactttaatt tctcctctaa aagctccctg
     4981 gactctttaa tttgcggtgg tttcgattct aataggctaa ttaattgatt tttaaaatcg
     5041 tcaaactctt ttaatcgatc attgtgcaca tctaccaacg ctggtttttg acgtaatata
     5101 tcaaacttag atttaactac ttcagataaa ttctcgccca gtttttggga aagaatttca
     5161 gcatcatcat tcagtctagt tattttaccc tccaaaattt tttttatggt ctcatctatt
     5221 tttcccctaa catccgttgc cagttgatta tcttccagat cagcactttt gaatgagtca
     5281 attgctcctt ttactgaatt aaataatgca tttctttcgt catcgctcat cgatttatgc
     5341 ttatcatgga aataaatatt acgaacttca ctgataagtc cctctaagct attgactaaa
     5401 atattaaatc cttcttcgct ttgaatcaca ggtgcgtatt tatcattaat acatgattcc
     5461 gaaagagatt gagcctctgc aacgtccttc gtcacttgag atgaatgcct aattatttca
     5521 tttaatctct ctactgcttt atcaatgact tcattgagtg gcagtccatc ttctaacgca
     5581 agctctttat taatagtaag acgggcatga tcataattta caaaatgacc gccaggaagg
     5641 tggggcaaaa catcacttaa gaatgcatct ttataggctt ggtggagaat aatacgatca
     5701 tctcggtaac taccaaacac tttttgaatt gattgttccc gtccaagaac cgggtaggaa
     5761 gtgttattaa aatgctcgta ggcagctttg tttttgatta tatcctgata tagagtcaaa
     5821 tatttttctt taagcttttc tttcttgatc ttgccatctt catcaaacag tggtcctatc
     5881 aaatcgtcaa atgaaagatt attcacttgt ccttcatcac tgttttgtcc aggctttata
     5941 gcgactccat tgcgattcag aaaatctatc agctttataa tcattaattg ttgtgcgtgt
     6001 tctgtggttg tctcgccccc aggaacagct tgttgttgtt gatcctttag attgcctgag
     6061 ccagttccat ggtgtccagg cagactaaaa agattaaatt ttgtatcgct tgaatcacat
     6121 tttggaacaa tagccttaaa gcagcgtgtt ctctcatttt cataaacaca ctgctcatat
     6181 tccttaacta tcttgggtac tctaaaaaag cgctcatctt tccacccaac tggtgcgccc
     6241 aggaaatcac cgcctggaac cggatcaata ttgaatattg atagttttac attattgatg
     6301 ttattcgcaa taccctcaag attcagagat tcataagtat ctttctgagc ctccatcgcc
     6361 gttttggtta gcggacactt actatttttc aaatcggcag aacctttacc ctgcttaaaa
     6421 ttttcctgaa ttcgttccag ttcatgagcc accaaaatcg cttctactgc acctcgacta
     6481 tgagccataa tactaatgtc tgtttcgcct cggctaattg cctctaatat gctagctaca
     6541 ccacgtgcga tccggtcacc aactgctttc cccatagtgt ctgggccatc aactactgcg
     6601 atatttgaat ttctatagtg agttatgtta tcagttaatt gcggctcacc cggcctatca
     6661 accagagtag atacgtaact caatgtttct ccatgctcat atccagtgtc aggttttgga
     6721 gtaaaagtgg tatctgtacc caataaagta agtgtgtatg ccatgatgta agctcctcag
     6781 tgaacataat gacagtttat attttataca ttaaggtcaa attactttca catagaataa
     6841 aagcatgggg tattggaaaa acaaataata gccaataggg ttagatggga tatcagactt
     6901 atttctgagg aaaaagttag aaattataag ctcatccaaa aatccttacc atttaccaag
     6961 aaaactgagg atctcagggt tcctcgcgct atgatttttt ctgaaagatt tagcctgcca
     7021 gctctatgaa taaaaacaat attgtgggat aattattgtc caacttcaac aatattagcc
     7081 tatttatatg aaaataagag caattttttt cataatcgct atttttcttc catttacttt
     7141 atttgccagt gatgcgactt gtcctttatc aaatggaatc aatgtactca ctaaaaagcg
     7201 aattttaaat atttgtaaaa acggcaccgt cgttaaaacc tttaaagtag cactcgggta
     7261 caaaggcgtt ggcaaaaaaa aggctgggga taacaaaact ccaattggtt tatatgggtt
     7321 agcccatcca aggaaatcca atcaatttaa agtctttatt ccaattctct atccgactaa
     7381 aaagcaatta gccgcaggat atactggtag agatgtcggg atccatggac caacccagtc
     7441 gcccggcgtg tttagttggt taaacaactt acccggctca actcgtggat gtgttgctgt
     7501 tggcaaaaac aactacatcg agtatgtggc aaattgggta aaagccaatc ctggagctaa
     7561 ggttttaatt atttaaattg agacgaaatt tgataccgcc tatttatttt atgtagaatg
     7621 ccctgatagt ggtcgtcttg atgataaaga aaattcattt aaaccaggta agtgctctaa
     7681 cgaattgcgg atttgtctaa aattaaggct acccgtagca ttaaatattt ttagaacttc
     7741 agcaactaaa ggtctctctt gtcgggcccc atcttttatc acattgcgaa tcaggcgaag
     7801 tgcatcacta attgattgct cttcttgtgg gtctatttct tttataatcg ctttgatttc
     7861 ttttaaaatt ttgagaattg agccgtcttc attacacatt aaaggacact ctagcaagtg
     7921 tagagtgcga gtccatctga actcctcaca tcaaatagaa ggtttaatct aataaggccg
     7981 gtctttatgg ctgtgtttta cttaacccag atatcaattt ttttggtaat aaaaactctt
     8041 ggtatttgtt ctatactttt atttaaatat aaatatactt agtttggaat tcatatgcct
     8101 ttgtatgtat cagactgtca atcgaatttc tcggtgataa acaacgggca ccatttactt
     8161 caccagaagc aagccgggat cgagaaatcc aaaataacac aaactgctgc tcaagcagct
     8221 aataatacaa actttgcaaa agtaaccatt gaatctgaat acgatgtcga tagtgaaaag
     8281 aaagtaactc aagtctgaga ggtaagaaga ttttttatac ccggattaat tcaaaaggat
     8341 tattttaata tcaatttgat ttttataacc tgagaggatt gaagtggaca tagtaaaagc
     8401 acagaaaaac atgaaggtaa aagtaaatgt tttaagaatt ccagccaatg aaagagaagc
     8461 taacatagta gctgtttatt cgatattaat taacaaggac ttgatgggag atatggatca
     8521 tatcccaaat gtcatttggc aaataaaatc aattattgaa aatatcaatc tggatgatga
     8581 tgatgatatt gcaagatcaa tttgtctaat taaagaaaaa atagaaaact ccaatgaaaa
     8641 ttataccaat aaaaatatca tggattttct aaatgcattt agtaaaaact cagatttgac
     8701 tttcagacaa atccgacagg aattagcaca atccaattct gaaatgaaaa aaattctgga
     8761 tacctatgat tgatgatgtg attcatagac atcatttcaa ttcattccta ttatccactg
     8821 tttatgaata agcaaaaaag cataggctaa tttttacctt gatactaaaa atacaggctc
     8881 tgtctgccat cgatggtatc tcttgtcaga cagataacca agaaaatggc aatgcagata
     8941 tttagagagg aaacagggta ataagctaac aaattaagaa caaaggggct ggaaagaagc
     9001 ttgttgatta aaaagctatt ttacttatgt agcgcggtat tgaacaatcg aaaattattt
     9061 tttcccccta ttttcaccct atataatgct tcatcagctt ttctaattaa tgtgtctaaa
     9121 tcatagccat catatggata gatacttata ccaatactgc ctgtaattga aataccataa
     9181 cgtttaattg ttataggctt gcttatagca attagaattt tatttgctat ttctataact
     9241 tgatttacat tggataattc agtaagagcg atgatgaatt catcgccacc atacctgaca
     9301 attagatcag tagaccttaa aatagttttt aatctttttc caatctcaat taataaaaga
     9361 tccccaatat catgccccaa tttgtcattc acatttttaa aataatccaa atccataaac
     9421 ataacagcaa tttgcttttg gtgacgtttc gcatgtgcta atgcctggtc aaatgataac
     9481 tctaatagct ttctatttcc caaaccagtt aaaatgtcat gatatgcgat gaattgaagt
     9541 tcttgcgctg ctcgcttttg agcatcaatg ttttgaattt gcgagataaa atagagtggt
     9601 ttattctcgg gatctcgaat taaagaagca ctgagtaaaa tccaaatgac gctaccattt
     9661 ttgcgaatat agcgtttttc catatgatag aatcttatat ccccctctaa taattgcctc
     9721 acatagccca aatcgagctc taaatcctct ggataagtga tcgattgaaa attagttttc
     9781 aataattctt cttctgagta tcctacaatc tgacaaagcg attgattaac ctttaaccac
     9841 atcccctcta aagaaactat agccatacca atagcagcag aattaaatgc ggaacggaaa
     9901 cgtaactcac tttcctccaa attagcgtta attgcataca atttattaat taaatccaaa
     9961 ttctcataaa gtaatttaaa attcttatta agaagtccat atcccatcca ggatataatt
    10021 gataaaaaac aaaagtaaat cagcaatgca gttccaagga accaatagtt aagtgtattt
    10081 tgctggaaaa accagacaga tagaggaata agggttaatg taaaaaaaaa tagacttgct
    10141 aaaaaactgg gctgaagaat atgtaatcca cctgatgcga ttccaataat tataattatg
    10201 acaatcattt gatttaacag atcactctga ggaataagaa ccgaacctgc tattccccat
    10261 agaaccccat aaatcgcagt gacgcttatt aaccatttta aaaggtattt ggatggcaag
    10321 ggacgataat aattaaataa aaataaacct ccatggagag caaatactgt tactccaacc
    10381 ataaaccata caaagagaat ttcttcatct attgttttat ccaaaccaat ataaataatt
    10441 aatgcacata aaagatttgc aggaatgctc actaataaat ttttatttag taacattacg
    10501 cgatctgaaa acattatttc atttatattt tgaatctttg ctttggcctt ttgcattcag
    10561 cactcactga gataaagtga caaattcaaa acctcaattt ga
//
LOCUS       DATNSN010000117        10507 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.117, whole genome shotgun sequence.
ACCESSION   DATNSN010000117 DATNSN010000000
VERSION     DATNSN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.117"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            37..588
                     /locus_tag="VAH76_002300"
     CDS             37..588
                     /locus_tag="VAH76_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEO1386902.1"
                     /translation="MLYHDPLISEHTMKWVRTFIINYTICPFAKGPVNKGTLRIITSD
                     TPKKHQALKDLITEIQFLEANPAVETTLLIFARAFKDFFSYLDFISLAEQLIQNLNYE
                     GIYQLATFHPDYYFADTSPDDVANYTNRSPYPMIHLLREDSLDKAIAAYGDTHTIPEK
                     NIATMNELGLEKMKQLLASISIP"
     gene            complement(621..1859)
                     /locus_tag="VAH76_002301"
     CDS             complement(621..1859)
                     /locus_tag="VAH76_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEO1386903.1"
                     /translation="MSSILFKNANVILGESTEIQKNFDVMVQNDLIAQVSQTPLQPLE
                     GMRVIDVKGKTLMPGLIDAHAHVTGLTLSPKNIFYSEAEIFLAAANYLKNSLFYGFTT
                     LREAGGADFRIAQLLDNKSIPGPRLFYSGKALTQTGGGADFRKPNEQIDPCGHVSSFS
                     TMSVIADGVDEVRKAAREELRKGATQLKVFASGGVVFPSLSNPTLYEYSEEELSTIVE
                     EAHARNTYVMAHAYSDESVRKCIKSGVRSIEHANFVSEPTVELMSESGVVYDPTFISL
                     AQRIESAEQNRLSKAIVANLKNTIEKGKKVYEYALKYKIPIAFGTDLWGPEAQRDQLR
                     EFEMRKELDSAANIIRSATVVNAELLMQKGKLGVISEGAYADLLVVDGNPLVNLNVLL
                     RPDENLKLIMKDGVIYKNEL"
     gene            2060..2620
                     /locus_tag="VAH76_002302"
     CDS             2060..2620
                     /locus_tag="VAH76_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386904.1"
                     /translation="MNTQDPALSLSNPRLLSAIYFGLLSVVGTILINAFLTSIGIEEI
                     IPVYQSVILGMVVASCTGAIFGEAIVHCKKPYKRKTFWLGFSMVIASLPVFDLGLVFL
                     MSEDNAKLFEVTNLRSMVLFYLVVLGYSYVLFGILLAVASGLAAMYLRGQLVYDILHT
                     QTRRQQKHKHALAKSKSEGHAHKVHR"
     gene            2598..5144
                     /locus_tag="VAH76_002303"
     CDS             2598..5144
                     /locus_tag="VAH76_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEO1386905.1"
                     /translation="MPIKFTDELKSDELYNLLSQGAVVITPNNRLSGAILQNYFTYCS
                     RKTITKPMCLPFNTFLVNSFEQVKFQKPDLEHPILLNSSQCQHLWRKLIKSHPSIIYT
                     EGLLKTIMQAWEYCEQWQLTPEAPAFHYTPQTQQFQQWWQSFNKTLKEISAIHQHQLI
                     PYLLDSNYQGLAKTVVWMCFDDFNPQQLSLQNYLNAQGVIQYGYDLKENQETPEVFSA
                     KDKKTEYQELMTWLEKKIQQGKKQIGVVVPNLEQESSVLQRILRDCFEPNLFNISLGK
                     PLSAFHLISHALIWLSLDHNLLNNHQAALLLQSPYLGKAKDEFLQRSDYLQESKLLQD
                     QSFPTKLFIEDIAYHTPKLAELLKNMNFYPEKTTLDEWIELFQERLNGIGFPGDYVLN
                     SEDYQCFNRFTMIFDEFRQLSLISSHLTKTEAFETLKQLIDNTIFQPQKSNSPIQILG
                     LLEASGCEFDSLWVMGLTDRCLPQQTRLSPFIPFELQRELGMPHSIPARELQYAKQIL
                     QRLQRGSMETVFSYPQFDDDTPNLPCSLIIHYPAYLSRPADNKTNVQSCLVPLEENYE
                     VPVKPDESISGGTSLLSNQAKCPFKALAQHRLKANPVLKTTDGMDDKEKGQLIHKILE
                     LLWQRLENQEHLIKLKPAELERFIDQAIQGALNPIRQRHSQAFPDLVANVEYMRLKRI
                     ILSYLEWEKQRPPFKITGLEENHSINLAGLDFKVRVDRLDEVGNKQWVIDYKSTLPGG
                     KPWNEERPMEPQLLLYALLNEQINTLLLIQLKTGKIKLSGFSEEKWDTKEINTLKEDE
                     SWEDYRQRWLEQLTHLAEEIKSGYCSPRPQNLNICQRCDFQNLCRFQAKQ"
     gene            5172..6014
                     /locus_tag="VAH76_002304"
     CDS             5172..6014
                     /locus_tag="VAH76_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO1386906.1"
                     /translation="MHHLILGYGYCGYYLAQELLGHHQQVTVVSRQLKKELELSKIQH
                     ISHDLEYPFHWNEPDSIIYYLIPPSSQGTCDTFLQRFLNQSSLNAKKIIYFGSSGVYG
                     NHQGAWVDELSACIIDSPRQQRRLDAEQQWLAYCQQHSRELSLLRIAGIYGPNRIPVD
                     AAKSKIPIIKPTEAPFTNHIFVKDLALIAYLLGQKQTPSAVYNVADGNPTHMGELQQT
                     VANALGIESASYESFEQAWEKASPMKREFMRASKRLKIDMLKAALGEDIQLTCLYDAV
                     KQSL"
     gene            6166..6816
                     /locus_tag="VAH76_002305"
     CDS             6166..6816
                     /locus_tag="VAH76_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2271 family Dot/Icm T4SS effector"
                     /protein_id="HEO1386907.1"
                     /translation="MPSYNNARLLEKRLQNCDTQLNQFFNGQELSIKFLQQLNQIKYF
                     YNSAFNKTENEDDGLEVVEEYENFISLVSQVKSGQINADKAFETIKDTTESRQADVII
                     ANFFKVCELMFWATAAFFSYVACVSVGIPLTFCDPLLGIAVSLSTFLMAISTASKFLD
                     CFDEFQSLDKINEHDQNQKDTVRFFSKTSISTSSVRNKEAEVEESSQRLYPNLQTI"
     gene            complement(6990..7478)
                     /locus_tag="VAH76_002306"
     CDS             complement(6990..7478)
                     /locus_tag="VAH76_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEO1386908.1"
                     /translation="MIIRFSKVLLVVAVSFFCLLTAFGNITDYSANFPAVVKVLAMSD
                     IFPDSTITYRAITSPVLHYVAFIIIVILELLTAIFCGIGAWKLFKARNESASVFNHSK
                     NWAIGGLTLGFVTWQVIFMSIGGEWFGMWMSPTLNSALTTAFHIFITILAVMIYLVIK
                     DE"
     gene            complement(7623..8936)
                     /locus_tag="VAH76_002307"
     CDS             complement(7623..8936)
                     /locus_tag="VAH76_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO1386909.1"
                     /translation="MKILFLLLLIFLSGIPAQAKPVELVFWHAMAGHLGDEVRLLADD
                     FNKSQNQYRVKPIYKGNYTETLTNFAAAFRARQAPSIVQIFEVGTSIMLAPPGVIKPV
                     DLLMSEQGISLPKDDFIQSVREFYSRDGQLMAMPFNLSAPVLYYNADILAKVGYGKHN
                     FPQTWSAMEIMAEKIKKAGYDCTYTTAYPGWVLFESFLAIHGLPITQGNPARAAFHTP
                     QLMAHFQRLKRWHDLHYFRYGGRVDDATILFTSSVCPLFSQSSGAYNSLSALVPFHLG
                     VATMPLDTGASPIRHANVAGGAALWAVGGQTEEQYRGIARFFAFIAKPEVQKRWHEHT
                     GYLPLGLKGVYANIVQSSQHPALLLARTDLEDNLPEKPYKHMGPQNQIRGINDEVLEA
                     MFADLMSPEEALNEATIRANHLLLRFARNTQRERVVDDKVGKGLG"
     gene            complement(8933..9652)
                     /gene="ugpQ"
                     /locus_tag="VAH76_002308"
     CDS             complement(8933..9652)
                     /gene="ugpQ"
                     /locus_tag="VAH76_002308"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO1386910.1"
                     /translation="MVLEKIIGHRGASGYAPENTMASFNKALSLGCRFIEFDVMCSLD
                     GEPFIIHDDNLKRTTNGKGEVGLVEADYLRSLDAGSWYSKQYKGEKIPHFTDVLKWLS
                     FSDVQANIEIKPYPGTVEQTTVSVLSHIHRHWPQNKELPLVSCFEWDALVLCRSIAPE
                     MPLGLLLHEWDKDWVQKAKQLECYSIHFNRKILTADRVKAVKDQGYVVCAYTVNRKRL
                     AKKLFDWGVDALFSDYPDLLK"
     gene            complement(9657..10352)
                     /locus_tag="VAH76_002309"
     CDS             complement(9657..10352)
                     /locus_tag="VAH76_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386911.1"
                     /translation="MRKIAWMLGLMVLTPFAWADNEKPELVLDKIFFQVSAKKWVTTQ
                     TAILNVNINATLSNADLVKARADIMDSLNKIAKGEWHLVQFDRSQDSSGLEKLYVQAQ
                     ARVDQSALTNIYQNAKSVSKPGAKYEISGVEFKPSLEETQVVRAKIREQLYQQVNDEI
                     DRMNKAYPKQNYSVSNLVFFEGDNIPQQPRAYQAKEMNALAMAAAPAPLTVSNELILT
                     AMVEAASNRKQGD"
BASE COUNT         3334 a         2263 c         1835 g         3075 t
ORIGIN      
        1 aggtacaata tccctttttt cgaaaccact taccctatgc tctatcatga tccattgatt
       61 tcagaacaca ccatgaaatg ggtgcggaca ttcattatca attacacgat ttgccctttt
      121 gcaaaaggcc ccgtaaacaa aggcacttta agaataatta cttcagatac ccccaaaaaa
      181 caccaggcac ttaaagattt aatcactgaa attcaatttc tggaagcaaa ccctgctgtt
      241 gaaacgactc tgcttatttt tgctcgagca tttaaagact ttttttctta tctggatttc
      301 ataagccttg ctgaacaatt aattcaaaac ttaaattatg aagggatata ccaactcgct
      361 actttccacc cggattatta ttttgctgat acttctccag atgacgttgc caactacact
      421 aatcgttccc cttatcccat gatccattta ttaagggaag acagcctgga caaagcaatc
      481 gcggcctatg gcgataccca cactatacct gaaaaaaata tagcaactat gaatgagctt
      541 ggtttagaaa aaatgaaaca gttattggct tccatttcta taccctagta tgcaacagga
      601 cttccgaaaa agaccatgct ttataattca ttcttataaa tgaccccatc tttcataatc
      661 aatttaaggt tttcatccgg gcggagcaga acatttaaat tgactaaagg attgccatct
      721 accactaaca gatcagcata agctccttcg cttatgacgc caagttttcc cttttgcatt
      781 aataattccg cattgactac ggtagctgaa cgaatgatat tggctgctga atcaagctct
      841 ttgcgcattt caaattctct taactgatct ctctgtgcct caggacccca caaatccgta
      901 ccaaaagcaa taggaatctt atattttaaa gcatattcat aaaccttttt gcctttttca
      961 atagtatttt tcaaattggc tacaatagcc ttagacaacc gattttgttc agcactttca
     1021 attctttgcg ccaatgaaat aaatgtcgga tcgtaaacca caccactttc cgacattaat
     1081 tctacagttg gttcgcttac aaaattagcg tgttcaatgg aacgcacacc cgatttaatg
     1141 cactttctta ctgattcatc gctataagca tgagccataa cataagtatt tcttgcatgt
     1201 gcctcttcaa caatagtcga taactcctcc tcggaatatt catacagtgt tggattgcta
     1261 agggaaggga aaacaactcc gccagaagca aatactttta attgtgttgc tccttttctt
     1321 aattcttctc gagcagcctt tctcacttca tcaaccccat ctgcaataac cgacatcgtc
     1381 gagaaagaac taacatgccc gcaaggatca atctgttcat tcggttttct aaagtcagct
     1441 cctccacccg tttgagttaa tgccttcccg gaataaaata atcgaggccc aggaatactc
     1501 ttattatcca gtaattgcgc aattctaaaa tcagcgcctc cagcttctcg taaagtcgta
     1561 aaaccataaa acaagctatt tttgagataa ttagcggcag ccaaaaatat ctctgcttcg
     1621 gaatagaaga tattttttgg gcttagggtc aatccggtaa catgagcgtg agcatcgatt
     1681 aaaccaggca ttaatgtctt ccctttgaca tcaataactc gcattccttc gagaggttga
     1741 agcggtgttt gcgacacctg ggcaatcaaa tcattttgta ccataacatc aaaatttttt
     1801 tgaatctctg tagattctcc caaaataacg ttggcatttt taaaaagaat tgagctcatt
     1861 ttaatctcga agtcattaat ctaacttact ctatttatag tctattttgg caactctgac
     1921 tcgctctcat ttttctttac cctgcttttt caaaagtatt taatttaaca gtgccccata
     1981 gaaaataatg aaacccgagt ccacattatt gaaataatgt actaatattt atcatataga
     2041 acttattatc aggtgcatca tgaacacaca agatccggca ctttctctct caaatccacg
     2101 gttattatca gcaatctatt ttggcttgtt atcggtcgtt ggtacgatac tgatcaatgc
     2161 attcctcact tcgattggaa ttgaagagat tatcccggtc tatcaatcgg tcattcttgg
     2221 aatggttgta gcttcctgca ccggggctat ttttggggaa gccatcgttc actgtaaaaa
     2281 accttataaa agaaaaacct tttggctagg attcagtatg gtcatagcct cactacctgt
     2341 ctttgatctc ggccttgtct tcttgatgtc agaagataat gccaagcttt ttgaagtaac
     2401 caacctaaga agcatggttt tattctattt ggttgttctg ggctatagtt atgtactttt
     2461 cggtattttg ctggctgttg catcggggct tgctgctatg tatttaagag ggcaattagt
     2521 atacgatatc ctgcatactc aaactcggcg tcaacaaaaa cataagcatg cattggcaaa
     2581 aagtaaatca gaaggacatg cccataaagt tcaccgatga acttaagtca gatgagcttt
     2641 ataatctatt gtctcaagga gcagtcgtta ttactcctaa caatagatta agcggtgcca
     2701 tccttcagaa ttacttcact tattgttcca gaaaaacaat taccaagcca atgtgcttgc
     2761 cttttaatac atttctggtc aactcttttg aacaagtcaa attccaaaaa ccagacctgg
     2821 aacatcccat acttttaaac tcgtcacaat gccaacattt atggcgaaaa ctgattaaat
     2881 cccatccttc tatcatttat acggaaggct tgcttaaaac aataatgcaa gcatgggaat
     2941 actgtgagca atggcaactt acacctgaag cgcctgcttt tcactacacg ccgcaaacac
     3001 aacaatttca acaatggtgg caatctttta ataaaacact taaagaaatt tctgccatcc
     3061 atcagcatca attaattccc tacttgcttg attccaatta tcaagggctc gcaaaaaccg
     3121 ttgtctggat gtgctttgat gactttaatc cacagcaatt atctctgcaa aattatttga
     3181 acgcgcaggg ggtaattcaa tatggttatg atttaaaaga aaaccaggag actccggagg
     3241 ttttctcagc taaagacaaa aaaacagaat accaggaatt gatgacctgg ttggagaaga
     3301 aaattcaaca aggaaaaaag caaatcgggg ttgttgtgcc taatcttgaa caagaatcat
     3361 cggttttgca acgaatttta cgcgactgct ttgaacccaa tttatttaat atttctttag
     3421 gcaaaccatt aagcgcattt cacttgatct ctcatgccct catttggctg agtcttgatc
     3481 acaatctctt aaataatcat caagcggctt tactgctcca atcaccctac cttggcaagg
     3541 caaaagatga gtttcttcaa agatcagatt acttacagga aagcaagttg ttgcaagacc
     3601 aatctttccc cacaaaatta tttattgagg acattgccta ccacacgcct aaactggctg
     3661 aattgttaaa aaacatgaat ttttatccgg aaaaaactac tctggatgaa tggattgagt
     3721 tatttcagga gaggttaaat ggcattggtt ttcctggaga ttatgttttg aactcagagg
     3781 attatcaatg ctttaatcgc tttacaatga tttttgacga attcagacaa ttaagtctga
     3841 taagcagcca tttgactaaa acagaggcat ttgaaacgtt aaaacaactc atagacaaca
     3901 ctatttttca accacaaaaa agcaattcac caattcaaat tttgggattg ttagaggcat
     3961 caggttgcga gtttgacagc ctttgggtaa tgggattgac tgatcgatgt ttacctcaac
     4021 aaacacgttt atcgcccttt atcccatttg aattacagcg tgaactgggc atgcctcaca
     4081 gtatcccggc aagagagctc caatatgcca agcaaatttt gcaaagactg caacgaggca
     4141 gcatggaaac cgtttttagt tatccacaat ttgacgatga cacgcctaat ttaccctgct
     4201 cattgattat tcattatcct gcctacctca gccgacctgc tgacaataaa acaaatgttc
     4261 aaagttgttt ggttcctctt gaagaaaact atgaagtacc tgtaaaacct gatgaatcaa
     4321 tctctggcgg aaccagctta ctaagcaatc aggccaaatg cccttttaaa gcattagcac
     4381 aacaccgact taaggcaaac cctgttctaa aaactacaga tggaatggat gacaaggaaa
     4441 aaggtcaatt aatccataaa atcctggagt tgctctggca aagattggaa aatcaggaac
     4501 acttaattaa attaaaaccc gctgaacttg aacgattcat tgatcaagca attcaaggcg
     4561 ctttaaatcc gattaggcaa cgacactccc aggcttttcc tgacttggtt gccaatgttg
     4621 aatacatgag attaaaacga attatcttgt cttatctgga atgggaaaag caacgccctc
     4681 cttttaaaat cacaggcctg gaagaaaatc attcgatcaa tttggctgga ctcgatttta
     4741 aagtaagagt ggacagactg gatgaagtag gtaataagca atgggtcatt gattacaaaa
     4801 gcactctccc tggcggcaag ccatggaatg aagaaagacc gatggagcct caattgctac
     4861 tttatgcctt gcttaacgaa caaattaata ctttgttatt gattcaatta aaaaccggaa
     4921 aaatcaagct cagcggtttt agcgaggaaa aatgggatac caaagagatt aatactttga
     4981 aagaggatga aagttgggaa gactatcgac aacgctggct agaacaatta actcatttag
     5041 ctgaagaaat caagtctggc tattgctcgc cacgccctca aaatctgaac atttgccagc
     5101 gctgtgattt tcagaactta tgccgcttcc aggctaaaca ataaaccagt tttaatttat
     5161 cgggactact tatgcatcat ttaattcttg gttatggcta ttgcggttat tacctggctc
     5221 aagaacttct tgggcatcat caacaggtaa ccgtagtatc acgacaatta aaaaaggagt
     5281 tggagctttc aaaaatacag catataagcc atgatctcga ataccctttt cattggaacg
     5341 aacctgactc cattatctac tatctaattc ccccgtcttc gcaaggtacc tgcgatactt
     5401 tcttacaacg gtttttaaat caaagttcat taaatgcaaa aaaaattatt tactttggtt
     5461 caagtggagt ctatggaaac catcaaggag cctgggttga tgaattatca gcttgtatta
     5521 ttgactctcc acgtcaacaa agacgattgg atgcagaaca acaatggctg gcctattgtc
     5581 agcaacacag tagagagctt tccttattgc gtattgcggg catctacgga ccaaacagaa
     5641 ttccagttga tgcggccaaa tcgaaaattc caataataaa accgactgaa gcccctttta
     5701 ccaatcatat ttttgtcaag gatttggctt taatagctta tttgcttggc caaaagcaaa
     5761 caccatcggc tgtatacaat gttgccgatg gcaacccaac ccatatgggc gaactacaac
     5821 aaacggtagc aaacgcatta ggaattgaat ctgcatccta tgaatccttt gagcaagctt
     5881 gggaaaaagc aagccctatg aaaagagaat tcatgcgcgc gtccaaacga ctgaagattg
     5941 acatgttaaa agctgcatta ggtgaagata ttcagcttac ctgtttgtat gatgcagtaa
     6001 aacaaagctt atgattttct gtttttgtaa aaaactattc ttgctaagtt aaactttaat
     6061 tttctttaaa atatggtatt atgctgccac attggttata tataatccaa taattcgtta
     6121 taaatcaata ggattttaaa tttataatta caattgaggt aaactatgcc ttcttacaac
     6181 aatgcccggc tcttggaaaa acgcttgcaa aactgtgata cacaattaaa tcaatttttt
     6241 aatggccagg aattatcaat aaaatttctc caacaattaa atcaaatcaa gtatttttat
     6301 aattcagctt tcaacaagac cgaaaatgaa gacgatggcc tcgaagtcgt tgaggagtac
     6361 gaaaatttca tttcgttggt ctcacaggta aaatctgggc aaattaatgc tgacaaagcg
     6421 tttgaaacaa taaaagatac gacagaatcc aggcaggcag acgttattat tgctaatttc
     6481 tttaaagttt gtgaactcat gttctgggct actgcagcat tttttagtta tgtagcctgt
     6541 gtatcagtag gtatcccctt aactttttgt gatcctctct tgggtatcgc agtctcctta
     6601 tctacctttt tgatggcaat tagcacagca agcaaatttt tagactgttt cgatgagttc
     6661 caatcattag ataagattaa cgagcatgat caaaatcaaa aggataccgt acggtttttc
     6721 agtaaaactt caatctccac ttcatcagta cgcaataaag aagctgaagt agaagaaagc
     6781 tctcagagat tatatccgaa tttacaaacc atctaaatta ataaacaata ccacatcaga
     6841 ccaaagatta ttttggtctg atgttttggt ttgctttaaa gagcataatc aaaattaagg
     6901 caatactgag tcacctcatg tttaaatatt ctctaaacag ccgttccgat aggaggctac
     6961 tctgttacaa attaatctct ggaatctctc tattcatcct taatcaccaa ataaatcata
     7021 acggctaaaa tagtaatgaa gatatgaaaa gctgtggtaa gggctgagtt gagcgtggga
     7081 gacatccaca taccaaacca ctctcctcct atggacataa aaatcacttg ccaggttaca
     7141 aaacccaacg taagacctcc aatagcccag ttcttggaat gattaaaaac agaagcactt
     7201 tcatttcttg ccttaaacaa tttccacgca ccaattccgc aaaatatggc ggttaataat
     7261 tccaggataa caatgataat aaatgcaaca taatgcagaa ctggactagt aattgctcga
     7321 taggtgatgg tggaatcagg aaatatatcg ctcatggcta atacttttac cacagcagga
     7381 aaattggcgg aataatcagt gatgtttcca aatgcagtaa gtaaacaaaa aaaagaaaca
     7441 gcaacaacaa gcaacacttt tgagaatctg ataatcatca tttcatcctt tgaaacaagt
     7501 taaaaaatat gtatcaccta attgataaga acgttcgtgg agaggatgct tatgctcgtt
     7561 tctcaccaag ccaggttcgc tttcttcaag ctttgctaac caaaccgatt aaccaaaaaa
     7621 tcttagccca aacccttgcc cactttatca tcaactaccc tttctctttg agtatttctg
     7681 gcaaaacgta acaataaatg attcgctctg atcgtcgcct cgttcagagc ttcctccgga
     7741 ctcatcaaat ctgcaaacat cgcctctaaa acttcatcat taattccacg aatttgattt
     7801 tgtgggccca tgtgtttata aggtttctca ggtaaattgt cctcgagatc tgttctggct
     7861 aataataaag cgggatgttg acttgattgc acaatgttag cgtaaactcc ttttaacccc
     7921 aacggcaaat aacccgtatg ctcatgccag cgcttttgca cttccggttt tgcaataaat
     7981 gcaaaaaatc tggcaattcc tcgatattgc tcttctgttt gtcctcctac agcccataaa
     8041 gcagcacccc ctgccacatt cgcatgtctt atcggactgg cccccgtatc caaaggcatt
     8101 gttgcaacac ccaaatgaaa aggaacaagg gcagacaaac tattataagc ccccgatgat
     8161 tgactaaata aaggacaaac actactggta aataaaatgg tagcatcatc aacacgacca
     8221 ccataacgaa aataatgcaa atcatgccag cgctttaatc gttgaaaatg agccattaat
     8281 tggggagtat ggaaagcagc acgagcagga ttgccttgag ttatgggcaa gccatggata
     8341 gcaagaaaag attcaaataa cacccatcca ggataagccg tggtataagt acagtcataa
     8401 cccgcttttt taattttttc tgccattatt tccattgctg accaggtttg agggaagtta
     8461 tgcttgccat atcccacctt ggctaaaata tcagcattat agtacagaac tggtgccgag
     8521 agattgaaag gcatcgccat taattgccca tccctgctgt aaaactcacg cactgattga
     8581 ataaaatcat ctttaggcaa actgatcccc tgctcactca tcaacaaatc aacgggtttg
     8641 atcacaccag ggggagctaa cataattgaa gtgcctactt caaaaatttg aactattgac
     8701 ggtgcttgac gagctctgaa tgcagcagca aaattggtaa gagtttccgt ataatttcct
     8761 ttgtaaatcg gtttgactcg atattgattc tgacttttat taaaatcatc agcaagcaaa
     8821 cggacttcat cacccaaatg ccctgccatc gcatgccaaa aaaccagctc aactggtttg
     8881 gcctgagcag gaatgccact cagaaaaatt aacaaaagca aaaagagtat tttcatttta
     8941 acaagtctgg ataatcacta aagagagcat caacacccca atcaaataac tttttggcca
     9001 aacgtttacg gtttacagta taggcgcata ctacatagcc ttgatcctta actgccttca
     9061 ctcgatcagc agttaatatc tttctattga aatgtattga ataacattcc aattgtttcg
     9121 ccttctgtac ccaatcttta tcccactcat gcagcaagag ccctaaaggc atttctggag
     9181 caatactgcg gcataatacc aaagcatccc attcaaaaca ggaaacgaga ggtaattctt
     9241 tattttgggg ccaatgccga tggatatgac ttagcactga tacagtagtt tgctcaaccg
     9301 ttccaggata aggttttatt tctatgttcg cctggacatc ggaaaaagac aaccacttta
     9361 atacgtcagt aaagtgtgga attttttctc ccttatactg cttcgaatac caagagccag
     9421 catcaagact acggagataa tctgcctcca ccagtcccac ctctcctttt ccattcgtgg
     9481 ttcgtttcaa attgtcatcg tgaatgataa aaggttcacc atcaagactg cacatcacat
     9541 caaactcaat gaaacggcaa cctaaagaca atgctttatt aaaagacgcc atcgtatttt
     9601 ctggagcata acccgatgct ccccggtgac cgataatttt ttcaaggacc aactaattaa
     9661 tctccctgtt tgcgattgga tgctgcttct accatagcgg tcaatattaa ttcattactc
     9721 actgtcaatg gtgccggagc agcagccatc gctaaagcat tcatttcttt cgcttgatag
     9781 gctcttggtt gctggggaat gttgtcgccc tcgaaaaaaa ccaggttact cacagagtaa
     9841 ttttgcttgg gataggcttt attcatacga tcaatttcat cattcacttg ttgataaagc
     9901 tgttcacgaa tcttggcacg tacgacttga gtctcttcca aactgggttt gaactccaca
     9961 ccactgatct cgtacttggc tccaggttta ctgaccgatt tggcattctg ataaatatta
    10021 gtcagagcag attgatcgac tcttgcctga gcttgtacat aaagtttctc aaggccagaa
    10081 ctatcctgag agcggtcaaa ctgcaccaaa tgccactccc ctttagcaat tttgtttaag
    10141 ctatccatta tgtcagcacg tgctttcacc aaatcagcat tacttagtgt ggcattgata
    10201 ttgacattga gtatcgctgt ttgggtagta acccattttt tagcagaaac ttgaaaaaaa
    10261 atcttatcaa gcaccagctc tggcttctca ttatcagccc aagcaaaagg agttaagacc
    10321 attaaaccca gcatccaggc aatttttctc ataatatgct ccattcataa tgtttctatt
    10381 ggaattagtg tataccagca agctagaaga caccacagca gtttgaacat aatcactcta
    10441 aaacaaatcc tatacggtat tgcccgcaaa cggtcggatt tgagcgcagg ttgagaaaat
    10501 aaacaga
//
LOCUS       DATNSN010000118        10466 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.118, whole genome shotgun sequence.
ACCESSION   DATNSN010000118 DATNSN010000000
VERSION     DATNSN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.118"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(83..919)
                     /locus_tag="VAH76_002310"
     CDS             complement(83..919)
                     /locus_tag="VAH76_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1386912.1"
                     /translation="MVLFMNIEYQLFEAGYCKHCERMTLKNGKIRQREYPSFCTLIKH
                     PSHGYILFDTGYSDRFTCLTQKFPASIYRHLTPMTLVKSLKEQLLEIHIKPEDINYIV
                     ISHFHADHIGGLCDFPNAHFICHSDAIDDIQHTKGLKALLKGFLPGLLPEDFFERLLP
                     LQKTIQLDEHLLPFSDGFDVLGDNSLIAIPLPGHAKGQIGLYFKAPGDIFLVADSCWH
                     QETFKELIYPSALTYLIHHDKRAYLKTIQKLHELYQRNKTIEIIPSHCQQLRSRIGNP
                     LC"
     gene            complement(904..1884)
                     /locus_tag="VAH76_002311"
     CDS             complement(904..1884)
                     /locus_tag="VAH76_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO1386913.1"
                     /translation="MVSVVTGATGCLGLNLTRRLIKEGHAVIALGRNLQLGEIISQMG
                     ATFVPLDLKDLESLSLISQNADFIFHCAALSSPWGKYKDFYQANVIGTHNVVQATPSQ
                     ARLVYVSTPSIYFDFTEKHNIKENALLPAKPVNYYVQTKLIAESIVDKAQLQRGLDVI
                     TIRPRGIFGPYDRAIFPRLLKAERQGVLPIIGSGNHLIDITFVENVVESLILAALADK
                     CYSGKKYNITNDEPRTFIDIISRMFSALNKPLKTRSIPYNQARFAAKFLEFLHRVLYL
                     KTEPKITEYGVGVLAFGQTLNIEEAKKDLKYKPIYSIDEGIMKFAEWYCS"
     gene            complement(1886..2869)
                     /locus_tag="VAH76_002312"
     CDS             complement(1886..2869)
                     /locus_tag="VAH76_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEO1386914.1"
                     /translation="MSSAKIIGMGKYLPANIVFSSDLDKKLNLPKGSVQKKSGLVSRH
                     FARVSETTSYMASQAALHAVEQAGITLAEIDAIIGACGAGEQPIPCTAVLVQKQLGLD
                     NSGIACFDINSTCLSFLTALDAASYMLSGGRFNRILIVSSDIPSFGLDWSDLETCTIF
                     GDGAAACVVERSNDSSRILSAHMKTYSLGAAFCEVQAGGTFMPPASKYDKKYGLFKMD
                     GKRVFKLASQMIGPMQEELFAKASITLNDVDWVVPHQASLLAMHHIRKKLGIPGEKFV
                     DIYTTHGNQMAASLPSALCHLIHNHNLERGQLVYLLGTGAGLSAAGMILEY"
     gene            3020..4186
                     /locus_tag="VAH76_002313"
     CDS             3020..4186
                     /locus_tag="VAH76_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO1386915.1"
                     /translation="MKILTVLDSYPPDLNGGAYFTHRLAKFLQAKGHEVLVICPSRSL
                     KQGYTSYEGVNLYGVRSWPTLGYKNFRVCWPFFIKKGILKAITDFNPDVVHLQGKFFL
                     GGICYRACRKEGIPLMATNHFMPENFFHYTHLPKYFEKWFHRTTWNIVIDMLNHVKIV
                     TTPTHTAANLLKEVQVQKEIHVVSCGVDLQKFQPKQNANLIRQRYKIPDKPVLLYAGR
                     LDKEKNLSIAIKAFYKARQSIDAHFVLTGRGAELQRLKKLVQTLNLTEHVTFTGYLSD
                     TEYPLIYSLANCFVNPGTAELQSIVALEAIASGLPLIAAKAMALPELVKEGVNGYLFD
                     PNDVETLSCYMVKILSDRTLSEQMGRESRKLSQEHDIKRTIEQYETLYQSMVSL"
     gene            4183..4839
                     /locus_tag="VAH76_002314"
     CDS             4183..4839
                     /locus_tag="VAH76_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386916.1"
                     /translation="MKTVANRYHVLCQEKVFIRSLLSGFFLLFISFLIMNFAGKYASL
                     MAGNPVNDLILDNLPLRDVTVFHVNAAIIFWTIFTIYTLLNPALIPFITKSAALFILI
                     RCAFISMTHLGAPPNQLAIPDNFSSLVLFDGDLFFSGHVGGPFLMMLIFWNKTTLRYI
                     CLAAAVFFAYTVLIGHIHYSIDVFAAPFITYGIFHISQFLFASDYQRLKSELTSSTGN
                     "
     gene            complement(4927..7929)
                     /locus_tag="VAH76_002315"
     CDS             complement(4927..7929)
                     /locus_tag="VAH76_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO1386917.1"
                     /translation="MIPRLTSSTATPRKIRLFLQQLEKIPDFHGEIDEALSSRIVLST
                     DNSVYQIIPEAIIFPKSESDISHIFKLASSEQFRDLTFTPRGGGTGTNGQSLNAGIII
                     DCSRFMNHIVEINPDEDWVLVEPGVVLDQLNEYLKPYNKFFAPALSPSNRATIGGMVN
                     TDASGKGSRLYGKTSQHILALTAVYLGGEVHTSEEIDVSLLDNMKKLPGQVGQFYKQL
                     DKTVQEHRETIDRQLPKLHRFVTGYNLAKIYSNDGKRFNANYILAGSEGTLAYVTKIK
                     LKLTSIPKYKCLFVVLYPSFSDALEHAKHLLAFDPEAIETMDSTILKLAKTDIIYDSL
                     KPFLNSGSNSQVDAINLIEFVANDEKELLHHAQLLKKNLKGTDFHFVTNNEDIINLWE
                     LRKRSVGLLGKVKGVRKPISGVEDTVVPPEYLADYVKEFRELLDSHGVSYGMFGHVDV
                     GCLHVRPELNLMDAKDREIYYEIIEKVAQLAHKYGGVLWGEHGKGFRSEFVPVFFGEE
                     LYNELRKIKSSCDPFNQFNPGKIAVPLGSHDSLASIRSEKTRADVDNQINYQYRDSYQ
                     LPLNCNGNGACFDYKLQDVMCPSYKISRDRIHSPKGRASLVREWLRQLSALAGKRELT
                     WPLKKRLNTYKRNHGQYDFSHEVYTALEGCLGCQACRSQCPVNINVPSFKAKFLQTYH
                     TRYSRSIKDYLIAYSERVGYFQSLFPELTNFCLSNKLVKYCLHKYTGLVDLPLFSSPN
                     LKSLLKANNIPWLDLNHLPVSEKTVIYIQDWVTSFYEAELVLKACQLIKKLGFQVYVL
                     PWFENGKTLHDNGMLQSFAKITRKNALMLKTLSDHGLTMIGLDPAMVLTYRYEYSEYL
                     HENYNSKILLIQEWLSQILGNENINELPIKANEKLKQYLLLSHCTEQANCSETLIQWQ
                     KVFQFFGLTLVAEKTGCCGMAGAYGHESIHQLNSHGLFDLSWKNYFEDSTGLSNTLVD
                     GYSCRAQVKRLTGLKASHPVETLLGLFE"
     gene            8085..8624
                     /locus_tag="VAH76_002316"
     CDS             8085..8624
                     /locus_tag="VAH76_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEO1386918.1"
                     /translation="MNKITEDSLTEEQKYMVQVWENHTHYEFYTKNVEDTMRTMEDNP
                     YVNNIPTMEGGFGKQEVRQFYSQFFIPQKPDDTEVELISRTVGTDQIVDELIFKFTHN
                     IRMDWILSGIEPTGKRVEIPQIAIVRFHNRKIISEHIYWDQASVLVQIGLLNSDKLPI
                     HGVEATNKIRELKSRSLIK"
     gene            8679..9527
                     /locus_tag="VAH76_002317"
     CDS             8679..9527
                     /locus_tag="VAH76_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386919.1"
                     /translation="MIKNYLIETMNQDEVSLAITWANKEGWNPGIYDVICFYQTDPHG
                     FFAGKLNGKTIAIGSAVIYDEFFAFCGFYIVDKPYREQGFGLELTKARLAYIGNRNAG
                     IDGVINMVDKYTRIGYQLAHNNARYCLNKTIKPISSEPHIVPLNQIDFSQLSSYDRLH
                     FPAPRPQFLKCWINQPQSLALAYLIDGQIKGYGVIRTCHKGFKIGPLFADTPEIAHQL
                     FTNLVPYAKDQLIFLDIPENNPLAIELVSQYQMEKVFATARMYLKGEPLVKKENIYGI
                     TTFELG"
     gene            9531..10421
                     /locus_tag="VAH76_002318"
     CDS             9531..10421
                     /locus_tag="VAH76_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO1386920.1"
                     /translation="MTRDLTGYGSEQINLTWPNKAKIAINFVINYEEGAELTPLNGDT
                     KAELYGSDFPFVPKGNAERNYSIESIYEYGSRVGIWRLIRLFDKYSIPLTFFVTGHAL
                     ILNPEFSGYLASHNHEVAGHGWRWIDYSKESKATEKKHISRCIDTLQELTGQRPLGWY
                     TGRRSPFTRDLLTQIGGFVYDSDSYADELPYFINKHLVIPYSLDCNDFRFTISPGFVT
                     AQDFYQRLKNTFDYLYQENRLSIMTIGLHPRLSGKPDRCHALNEFLSYIRQFQNIWIA
                     KRIDIALYWLTKHTQIKQDL"
BASE COUNT         3307 a         2003 c         1867 g         3289 t
ORIGIN      
        1 gcttgatgag tctttaattt ttccggggta gtaatagttt tattaagata aagtgtatta
       61 aaataatgaa atagaatacg tgctaacata atgggttccc aattctcgag cgcaattgtt
      121 ggcagtgaga aggaataatt tcaatagtct tgtttctctg atacagttcg tgcaattttt
      181 gaattgtctt taaataggca cgcttgtcat gatgaatcaa ataggtcaaa gcgctcggat
      241 aaatgagttc tttaaaagtt tcctggtgcc agcaactatc cgcaaccagg aaaatgtcgc
      301 caggcgcttt aaaatacaag ccgatttgcc ctttagcgtg tcccggtaaa ggaatagcaa
      361 tcaatgagtt gtcacccaat acatcaaaac catcagaaaa aggtaaaaga tgttcgtcaa
      421 gttggattgt tttttgaagt ggtaataaac gttcaaagaa atcttcgggt agcagtccag
      481 gtaaaaagcc tttcaataac gctttcaacc cctttgtatg ctgaatatcg tctatcgcat
      541 cagaatgaca tataaaatga gcattaggaa aatcgcataa tcctccaatg tgatcagcat
      601 gaaaatgcga aatcacaata taattgatat cttctggttt tatatgaatt tctagcaact
      661 gttcttttaa ggattttaca agtgtcatag gagttaaatg acgatatata gaggctggaa
      721 atttttgggt taaacaagta aaacggtcag aatatcctgt gtcaaataaa atatatccgt
      781 ggcttgggtg ttttataaga gtacagaaag atggatattc acgctgtctg attttcccat
      841 tttttaaagt catgcgttcg caatgtttgc aatagccagc ttcaaataac tgatactcta
      901 tattcatgaa caataccatt cagcaaattt cattataccc tcatcaatgc tataaattgg
      961 cttatacttt aaatcttttt ttgcctcttc aatatttaat gtttgtccga aagccaacac
     1021 tccgacacca tactctgtaa tctttggttc tgttttcaga taaagcaccc gatgcaaaaa
     1081 ctcaagaaac tttgcggcaa atcttgcttg attataagga atacttctgg tttttaatgg
     1141 tttgttaaga gcagaaaaca tccttgaaat gatatcaata aaagtcctag gttcatcatt
     1201 ggtgatatta tattttttcc ctgaatagca tttatctgcc aaagcagcca ggataaggct
     1261 ctccactaca ttttctacaa aagtaatatc aatcaaatga ttacctgaac caatgattgg
     1321 aagcacccct tggcgctctg ctttcaataa gcgagggaaa atagcgcgat catatggtcc
     1381 aaaaattcct ctgggacgta tggtaatcac atccaggcca cgctgtagtt gagctttatc
     1441 tacgatggat tcagcaatta atttggtttg cacataataa ttgaccggtt ttgctggtaa
     1501 aagtgcattt tctttaatat tgtgcttctc ggtaaaatca aaataaatac taggagtgga
     1561 gacataaaca agtcgagcct gactgggagt tgcctgaaca acattgtgag tccctatgac
     1621 attagcctga taaaaatctt tatattttcc ccaggggctt gataaagccg cgcaatgaaa
     1681 tatgaaatcc gcattttgac ttatcaaaga caaagactcc agatccttta aatcgagcgg
     1741 aacaaatgtt gctcccattt ggcttattat ttcacccaac tgcaaattgc gtcctaatgc
     1801 aatgacagca tggccttcct taatcaagcg tcttgtaaga tttaaaccta aacatcctgt
     1861 agcgccggtt acaactgaaa ccatactaat actccagtat cattccagca gctgacaatc
     1921 cagcgccagt acctaataaa taaaccaact gcccacgctc taaattatga ttatggatca
     1981 aatgacacaa tgcagaaggc agagaagcag ccatttgatt gccatgcgtc gtgtaaatat
     2041 caacaaattt ttcaccaggg atgccaagct ttttacgtat atgatgcata gcaagcaagc
     2101 tggcttgatg tgggacaacc caatcaacat catttagagt tatactagct ttagcaaata
     2161 actcctcttg catgggccca atcatttggc tggccagttt gaatacacgc tttccatcca
     2221 ttttaaataa accgtatttc ttatcgtact tactcgcagg cggcatgaat gttcctccag
     2281 cctggacctc gcaaaaggca gcacccagac tgtatgtttt catatgagca gaaagaatcc
     2341 gactggaatc attgcttctt tcaacaacac aagcagctgc cccgtcacca aaaatcgtac
     2401 aggtttccaa gtcgctccaa tccaggccaa atgaagggat atcacttgat acaataagta
     2461 tacgattaaa tctgccccct gagagcatat atgatgccgc atcgagtgca gtgagaaaac
     2521 taagacaggt actgttaata tcaaaacagg cgattcctga attatctaat cccaattgtt
     2581 tttgaaccag aaccgccgta catggtatag gttgctctcc ggctccacaa gccccaataa
     2641 tggcatcaat ttcagctaaa gtaattcctg cttgttccac agcgtgtaat gcagcttgac
     2701 ttgccatata ggaggttgtt tcactgactc tggcaaaatg cctggaaact aatccggact
     2761 tcttctgaac actgccttta ggaagattga gttttttatc cagatcagat gaaaacacta
     2821 tgtttgcggg taaatatttt cccattccga tgatttttgc tgacgacata gaactgactt
     2881 gttaaaggat ggagcaatta ttgtactata aggcttatta tagtcaaaga gaactttttt
     2941 aaaggttggt tggtaaataa ttgccgtgta tttgtataaa tgataatcta tctaatctta
     3001 tatattatct aaaatatgca tgaaaatcct gactgtactg gacagctatc ctcccgactt
     3061 aaatggtgga gcttatttta ctcaccgttt ggcaaaattc ttacaagcca aagggcatga
     3121 agttcttgtg atatgcccct ctcgaagcct gaagcaaggt tatacaagct atgaaggagt
     3181 caatctctat ggagtacgat catggccaac ccttggttac aaaaactttc gtgtttgctg
     3241 gcctttcttc atcaagaagg gtattttaaa agcaataaca gactttaatc ccgatgttgt
     3301 tcatttgcag ggaaaatttt tcttaggtgg aatttgctat cgagcctgtc gtaaagaggg
     3361 tatcccgtta atggctacta atcattttat gccagaaaat ttttttcatt atactcacct
     3421 tcccaagtat tttgaaaaat ggtttcatcg caccacatgg aatattgtga ttgacatgct
     3481 aaatcatgta aagatagtca ctactcctac tcacactgca gctaacttac ttaaagaagt
     3541 acaagtacaa aaagaaattc atgttgtttc ttgtggggtg gatctacaaa aatttcaacc
     3601 aaaacaaaat gccaatctca ttagacaacg ttataaaatc cctgataagc cagttttatt
     3661 gtatgctggt cgtttggata aagaaaaaaa tttatcaata gccataaaag cattttataa
     3721 agcaagacaa tccattgatg ctcattttgt tttaaccggt cgtggcgcag aattacaaag
     3781 attaaaaaaa ctggttcaga cactgaatct cactgagcat gtcactttta caggatatct
     3841 atcagacacg gaatatccac tgatatatag tttagcaaat tgttttgtaa accccggaac
     3901 tgctgaatta caaagtattg ttgccttgga agcaattgct tctggattgc ctcttatagc
     3961 tgcaaaagcg atggctttgc ctgaacttgt taaagaagga gtaaatggtt atctctttga
     4021 ccctaatgac gttgagacat tgtcttgtta tatggtcaag attcttagtg atcggactct
     4081 cagtgaacaa atgggaagag agagcagaaa attatctcaa gaacatgata tcaaacgaac
     4141 tatcgaacaa tacgagacac tctatcaatc gatggtgagt ttatgaaaac agtcgcaaat
     4201 cgctaccatg ttctttgtca ggaaaaagta ttcataagaa gtctgcttag tggctttttt
     4261 ttgcttttta taagttttct cattatgaat tttgcaggaa aatatgcttc acttatggcc
     4321 ggtaatccgg taaatgattt aattcttgat aatttacctc tacgtgatgt aactgttttt
     4381 catgttaatg cagctattat cttttggaca atttttacta tttataccct gttaaatcca
     4441 gcgctcattc cttttatcac taaaagtgcg gcgctgttta tattgatacg ttgtgccttt
     4501 attagtatga cccatctcgg tgcacctcct aatcaactgg ctattccgga taatttttca
     4561 tctcttgttt tatttgatgg agatttattt ttttctggac atgtcggagg ccctttttta
     4621 atgatgctta tcttttggaa taagacaaca ttacgttata tttgccttgc tgctgctgtt
     4681 tttttcgctt atacggtgct gattggtcat attcattata gtattgatgt ctttgcagcc
     4741 ccatttatta cttatggaat atttcatata agccaattcc tgtttgcatc agattatcaa
     4801 cgactaaaat cagaacttac atcatcaact ggaaattgat aacagttgcg tcatactgta
     4861 ggattagccc gcatgaggaa gtaattcagt aaatatatgg ctgagataat caattccagc
     4921 ttatccctat tcaaataatc ccagtaatgt ttcaacaggg tgagaagctt ttaaaccagt
     4981 taatcgtttg acttgtgctc gacatgaata gccatcgact aaagtattag ataaaccagt
     5041 actgtcttca aaataattct tccatgataa atcaaacaag ccatgggaat taagttgatg
     5101 gattgactca tgcccataag ctccagccat accacaacac cctgtttttt cagcaactag
     5161 agtcaatcca aaaaattgaa agactttctg ccactgtatt aaagtttcac tgcaatttgc
     5221 ctgttctgta caatgactta gcaataaata ctgtttaagc ttttcattag cttttattgg
     5281 taactcgttg atgttttcat tacccagtat ttgcgagagc cactcttgaa tcaacaggat
     5341 tttactattg taattttcgt gtaaatattc agagtattca tatcgatagg ttaataccat
     5401 ggctggatca agtcctatca tggtcaatcc atgatctgat agcgttttaa gcattaaagc
     5461 attttttctg gtaatttttg caaatgactg aagcattcca ttgtcgtgca gagttttacc
     5521 attttcaaac caaggtaata cataaacctg gaaccccagt ttttttatta actgacaagc
     5581 tttcagcaca agttcagctt cataaaaact ggtcacccaa tcctgtatat aaattacagt
     5641 tttctcactg acaggtaaat ggtttaaatc aagccatgga atattatttg cttttaagag
     5701 tgacttcaga tttggactac taaacaaagg gagatcaacc aaccctgtgt atttatgtaa
     5761 acaatatttc actaatttat ttgagagaca gaaattagtt aattctggaa acaggctttg
     5821 aaaatatcca acacgttcgc tgtaagcaat aagataatct tttattgagc gagaatatcg
     5881 tgtgtgatag gtttgcaaaa attttgcttt aaacgaagga acattgatat taaccggaca
     5941 ttgagaacga catgcctggc atcccaggca tccttctagc gcagtataaa cttcatggga
     6001 aaaatcatat tggccgtgat tccttttgta agtatttaat cgctttttca acggccatgt
     6061 taattctcgc tttcctgcta aagcacttaa ttgccttaac cattccctaa ccaagctggc
     6121 tcttccttta ggagaatgaa ttctatccct gcttatttta taagaagggc acatcacatc
     6181 ttgtaattta taatcgaaac acgctccatt tccattgcag tttaaaggta attgataaga
     6241 atctctgtac tggtagttaa tttgattgtc tacatctgct ctggtttttt ccgaacggat
     6301 gctggcaagt gagtcatggc tacctaaggg gacagcaatt ttcccaggat taaattggtt
     6361 gaaaggatca cagcttgatt taattttcct gagttcattg tagagttcct ctccaaaaaa
     6421 tacaggcacg aattcacttc gaaatccttt accgtgctcg ccccacaata ccccaccgta
     6481 tttatgggca agttgcgcca ccttttctat tatttcgtaa tagatttccc tatcttttgc
     6541 atccatcaag ttcaattcgg ggcgaacatg taaacaccct acatcaacat gaccaaacat
     6601 cccataacta accccatgag aatcgagtaa ctcacgaaat tccttgacgt agtcagccaa
     6661 atactctgga ggaacaacag tatcttcaac ccccgatatg ggctttctta cgccctttac
     6721 tttacctaaa agtccaacac tgcgctttct tagctcccaa agattgataa tatcctcatt
     6781 attagtaaca aaatgaaaat ccgtaccttt caaatttttc ttcaatagtt gagcatggtg
     6841 tagtaactct ttttcatcgt ttgccacaaa ttcaatcaaa ttaattgcat caacttggct
     6901 gttgcttcca gaattcagaa aaggtttcag cgaatcataa ataatatctg tcttggccaa
     6961 tttcaaaata gtgctatcca ttgtttcaat ggcttctgga tcaaatgcca gtaaatgctt
     7021 tgcatgttca agggcatcgc taaatgaagg atacaaaaca acaaacagac atttgtattt
     7081 gggtatactc gttaatttta atttgatttt agtgacatag gccaaagtac cttcagaacc
     7141 agcgagaata taattggcat tgaatctctt gccatcattt gaataaattt ttgccaaatt
     7201 gtaaccagtg acaaaccgat gcaatttagg taattgccta tcaattgtct ctcgatgttc
     7261 ctggacagtt ttatccaatt gcttataaaa ttgccctacc tgcccaggca attttttcat
     7321 attatcaaga agactgacat ctatctcttc actggtatgg acttctcctc caagatagac
     7381 agctgtcaat gcaagtatgt gttgactggt ttttccatac aatcttgagc ctttgccact
     7441 ggcatctgta ttaaccatac ctccaatggt agcgcgatta cttggggata aagcaggtgc
     7501 aaagaatttg ttataaggct tcaggtattc atttaattga tcaagaacta ctccaggctc
     7561 aaccaaaacc cagtcttcat ccgggtttat ctccacaata tgattcatga aacgagaaca
     7621 atcgataatt attccagcat ttaatgattg gccattagtg ccagttccgc cacccctggg
     7681 ggtgaaagtt aaatcacgaa attgctcgct actggctaat ttaaaaatat gagatatatc
     7741 actttcactt ttagggaaaa taatggcttc aggaataatt tgataaacac tattatccgt
     7801 tgataaaaca attcggctac ttaaagcttc atcaatctca ccatgaaaat cgggtatttt
     7861 ctcaagttgc tgtaaaaata acctgatttt tctgggtgtt gcagtggaag aggtcaaacg
     7921 cgggatcata attttttcct agcttttcct ccattaatct tctcattata gactaccaga
     7981 taattaggac taaatcataa agacggcaat cattcaatga gaaaaaaaag tctttttagg
     8041 ctaaattgaa ggaatagagc tattttttaa aggagtaaga caagatgaac aaaataacag
     8101 aagattctct gactgaagaa caaaagtata tggttcaagt atgggaaaac catacccatt
     8161 atgagtttta tactaaaaat gttgaagata caatgagaac catggaagac aatccatatg
     8221 tcaataatat acctaccatg gaaggaggat ttggaaaaca agaagtcaga caattttaca
     8281 gccaattttt tataccccaa aaaccggatg atactgaagt tgaattgatt tccagaactg
     8341 taggtactga tcaaattgta gatgaactca tttttaaatt tactcataat atcaggatgg
     8401 attggatatt aagtggaatt gaacctactg gaaaacgggt agaaattcct caaattgcaa
     8461 ttgtcagatt tcataataga aaaatcatca gtgaacatat ttattgggat caggcatctg
     8521 ttcttgtaca aatcggttta ctcaattcag ataaactacc cattcatgga gtagaagcca
     8581 ccaataagat aagggaactg aaatcacgaa gcctgattaa gtgagaaaaa tgcacttact
     8641 tctaactata attattgtta aagggtatag ggaccatcat gataaaaaat tatcttatag
     8701 aaaccatgaa tcaggatgaa gtgagtcttg ctataacgtg ggcaaacaag gaaggatgga
     8761 atccagggat ttatgacgtg atatgctttt atcaaactga tcctcatggt ttttttgcag
     8821 gtaagctgaa cggaaaaacg atagccattg gttctgctgt tatctatgat gaattttttg
     8881 ctttttgcgg tttctatatt gttgataaac cctataggga acaaggtttt ggtttggaat
     8941 taactaaagc cagattagca tatattggta acagaaatgc aggaattgat ggtgtaatca
     9001 acatggtgga taaatatact cgcattggtt atcaattagc acataataac gccagatatt
     9061 gtcttaataa gacaataaaa ccaatatcat ctgaacctca tattgttcca ttaaatcaaa
     9121 tcgatttttc gcagttatca tcctatgacc gcctacattt tccagcacct cgtcctcaat
     9181 ttttaaaatg ctggataaat cagccacaaa gcttggctct agcttatcta attgatggcc
     9241 aaattaaggg ttatggtgtt atcagaacct gtcataaggg atttaagatt ggaccactat
     9301 ttgcggacac tccggaaatt gcacaccaac ttttcactaa tttggtccca tatgctaagg
     9361 atcaattgat atttctggat attccagaga ataacccttt agcaattgag ctggtcagtc
     9421 aatatcaaat ggagaaggta ttcgctacag cacgtatgta tttaaaagga gaacccctgg
     9481 ttaaaaaaga aaacatttat ggaattacta cttttgaatt aggataaacc atgacccgag
     9541 atctcactgg atatggatca gaacaaatta acttgacctg gcccaataag gctaaaatcg
     9601 ccattaattt tgttattaat tacgaagaag gagcagagtt aactccactt aacggtgata
     9661 caaaggctga actttacgga agtgattttc ctttcgttcc taaaggaaat gcagaaagga
     9721 attatagtat cgaatctatt tatgaatacg gaagtcgagt tgggatttgg cgtttaatcc
     9781 gtctttttga caagtattcc atccccttga cttttttcgt aacaggtcat gccttaatat
     9841 taaatcctga attctcaggc tatcttgcaa gtcataacca tgaagttgct ggacatggat
     9901 ggcgatggat agattattca aaagaaagta aggcaacgga aaaaaaacac atctctcgct
     9961 gtattgatac cttgcaggaa ctcactggac aaaggccatt gggctggtac actggaagaa
    10021 gaagcccatt tacccgcgat ctgctaactc aaattggtgg gtttgtttat gattcagata
    10081 gttacgccga tgaactcccc tatttcatta acaagcatct tgtaatccca tactcattag
    10141 actgtaatga ttttcgtttc acaatttctc ccggctttgt cacggcacag gatttctatc
    10201 aacgtcttaa aaatactttt gattaccttt atcaagaaaa tcgattatct atcatgacta
    10261 ttggtttaca tccgcgatta agcggcaaac ccgatcgttg ccatgctctg aatgaatttc
    10321 taagctatat tcgacaattt caaaatatat ggatagcaaa gagaatagat attgcactgt
    10381 actggttaac caaacacacc cagattaagc aggatttatg aaatacccaa tctcattgtt
    10441 aggaaatgtt tttaattcac tcgttt
//
LOCUS       DATNSN010000119        10325 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.119, whole genome shotgun sequence.
ACCESSION   DATNSN010000119 DATNSN010000000
VERSION     DATNSN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.119"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(201..1505)
                     /locus_tag="VAH76_002319"
     CDS             complement(201..1505)
                     /locus_tag="VAH76_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO1386921.1"
                     /translation="MSLFNKEPDPPLAESLRPQHIDEVIGQSHLLGEGKPLRLCFMGS
                     KLHSMILWGPPGVGKTTIARLTAQAFDCEWIALSAVFSGVKDIRAAVEKAQEYLIHDK
                     QTILFIDEIHRFNKAQQDALLPYTESGLITFIGATTENPSFEVNSALLSRAQVYVLKP
                     LSEQELKLLFLRAHQRALSSLQFTEEAVDFLISCADGDARRLLNLLEQTKTACLTIKT
                     AVVDVELLHNVIVQNSRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLCRMLDGGVD
                     PYYLARRIIRMSWEDIGLADPKAIQIANDAAATYERLGSPEGELALAQAVIYLSVAAK
                     SNAGYVAFNQAMEFIKRDKSREVPIHLRNAPTQLMKKLGYGHEYRYAHDEPHGYAAGE
                     CYLPEGMPEVNWYNPVQRGLETKIAEKLAFLRQLDKDVKKKI"
     gene            complement(1521..2129)
                     /gene="lolA"
                     /locus_tag="VAH76_002320"
     CDS             complement(1521..2129)
                     /gene="lolA"
                     /locus_tag="VAH76_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEO1386922.1"
                     /translation="MNKLFLILLLIFSHEVFSQTSAEVLQSKLNAIQTMTANFSQIVK
                     AKNREVSRSSGSMALQRPGRFRWQTKDPLEQLIVADGQKMWIYDVDLEQVTVKNQEKG
                     LGGTAALFLSGYDETLTHDFDVSEKQKGKLTVFDLKSKSAKENFQRIKLIFSQSTLIG
                     LELYDQLGQITDVKLVQIKSNPKLPAKLFQFKPPKGVDVVKQ"
     gene            complement(2132..4516)
                     /locus_tag="VAH76_002321"
     CDS             complement(2132..4516)
                     /locus_tag="VAH76_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEO1386923.1"
                     /translation="MAKQHSGKQAGIPKKSMPSFIIKRLCEGSFILILTGALFVLLSL
                     FTYSTNDPGWSHASRSGMSVSNSGGQVGAYIADALYFAFGYFAFLLPVAFVYIAWAIL
                     KDFRSFKALDRMVLLLRTVGFILMVSGGCGLLSINHRFEAVDTIHSSGGIIGQTVGNG
                     WYQMLNMEGATLLLLAMFLVGTTWLTGLSWIKAIELIGCYTLNLFTFLDKFIRKGMQI
                     ISENFNKEKLKTPLIKTEQLPKPDNEKKKSVPKLFQDKKDKEQEKATPVLIASEEKPE
                     IVKPTNEFKEIRPPKTITPGALPSLSLLDKGQPGKPMGGYTHEELESLSRDVEQHLLD
                     FGIQADVVAVHPGPVVTRFELQLAAGVKVSKLTALAKDLARSLSVISVRVVEVIPGKT
                     VVGLELPNHSRQVVRLSDVLSADVYQQAHSPLSLALGVDIGGHPMVVDLAKMPHLLVA
                     GTTGSGKSVGINAMILSILFKASPEQVRLIMVDPKMLELSVYDGIPHLLTPVVTDMKE
                     AASALRWCVEEMERRYRLMAALGVRNLAGYNTKITEAAANGQPLLNPLWKPVDSMDET
                     APELQALPYVVVVIDELADMMMVVGKKVEQLIARIAQKARAAGIHMILATQRPSVDVL
                     TGLIKSNIPTRISFQVSSKIDSRTILDQQGAEQLLGHGDMLYLAPGSGAPLRVHGAFV
                     DDKEVHRIADDWRSRGEPDYVDDILKMGNENGDGAFDDDSGGQSVEDDDPLYDQAVEF
                     VIQTRKASISAVQRRLKIGYNRAARMIEEMERTGIVGPLDGGYRDVLVTSVTED"
     gene            4589..5539
                     /gene="trxB"
                     /locus_tag="VAH76_002322"
     CDS             4589..5539
                     /gene="trxB"
                     /locus_tag="VAH76_002322"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO1386924.1"
                     /translation="MSISNHHRLIILGSGPAGYTAAVYAARANLNPVLITGMQPGGQL
                     TTTTDVDNWPGDIEGLQGPALMERMQKHAERFDTQVIFDHIVKADLAQAPFTLQGDST
                     TYTCDALIIATGASARYLGLPSETAYQGRGVSACATCDGFFYRNKSVCVIGGGNTAVE
                     EALYLSNLASSVTLIHRRDSLRAEKILQDKLFEKAHSGNIKIIWNSTLDEVLGDGKKV
                     TGAMIRNLKTGETDQLAMDGIFIAIGHTPNTAIFQDQLAMRDGYILIKSGLDGMATST
                     SVPGVFACGDVADHVYRQAITSAGFGCMAALDAEKYLDSI"
     gene            5542..6210
                     /locus_tag="VAH76_002323"
     CDS             5542..6210
                     /locus_tag="VAH76_002323"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEO1386925.1"
                     /translation="MAYDSNYTFPDPETSDKQGLLAIGGVLTPKRVLQAYSQGIFPWY
                     EPGNPVLWWSPNPRLILIPNEFKISRSLKKTLKKPFKLTVDTAFQRVISYCATCSDRS
                     NKTWITSEMIETYTQLHEMGYAHSFEIWDGSELVGGLYGISLGHAFFGESMFHTITDA
                     SKVALHFLCRIMQSWNFDFIDCQLPTLHLMSLGAKIISRKEFLHMLQETLKYPDKKGN
                     WSID"
     gene            6265..6636
                     /locus_tag="VAH76_002324"
     CDS             6265..6636
                     /locus_tag="VAH76_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEO1386926.1"
                     /translation="MKQHSQGFLKLVTEAKKRINEISPQILKSKIDNNEKLIIIDVRE
                     TDEWETGKIPNAIHLSKGIIERDIEKIITDNQSNIVVYCSGGYRCALVADSLQKMGYT
                     NVLSLDTGLQGWLEAGYSLVK"
     gene            6767..6988
                     /gene="infA"
                     /locus_tag="VAH76_002325"
     CDS             6767..6988
                     /gene="infA"
                     /locus_tag="VAH76_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO1386927.1"
                     /translation="MAKEDHIEMAGTVIDTLPNTMFRVELENGHIVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLSKGRIIFRDKG"
     gene            complement(7155..8513)
                     /gene="pmbA"
                     /locus_tag="VAH76_002326"
     CDS             complement(7155..8513)
                     /gene="pmbA"
                     /locus_tag="VAH76_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEO1386928.1"
                     /translation="MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNY
                     DRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIA
                     KVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
                     DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENL
                     VSADLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNS
                     FLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSY
                     SARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSR
                     GASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVA
                     GK"
     gene            8559..9335
                     /locus_tag="VAH76_002327"
     CDS             8559..9335
                     /locus_tag="VAH76_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEO1386929.1"
                     /translation="MSSQTNVYPANKLNLWLLLVLALAIVIPMTSYAGSDWQELTPGI
                     EYQDLEGGLLNPWSHIHVFRIDLNKNQMALVTAKNLAQKNASVDQFAEHSKALLSING
                     GFFDHEFNPLGLRINNKKQENPLKRISWWGIFYVKDNKPRITNIRNFHYDSNIDFAIQ
                     SGPRLLIRGNIPSLKAGVADRTALGITDDGKVIILVTTNAAMSTRQLAQIMRAPPLSC
                     SDAINLDGGSSSQLYAKIDSFQLNVHGFSNVSDAIIVKKI"
     gene            9584..>10325
                     /locus_tag="VAH76_002328"
     CDS             9584..>10325
                     /locus_tag="VAH76_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEO1386930.1"
                     /translation="MKLKEIRTIIYNYGEEEILKHILYESKEPILIKIPDFTSQFSLD
                     YFEQLTQAKTTYCTFENKRCVDFQAGNFAEVIQQIKSNKPIRIFGQILSRERSKEIEK
                     HVPLWQKIPFRPRYFRETKKVAYFFGGKGAVSEMHFDREHCCNLHLCLSGKKQVLLFT
                     RKQSDNLYKLPFIGDSLIDFTQPLDALIQQYPRLNQAEGYQVLLEKGDMLFMPRNCWH
                     YTEYLDASSAATYVFYPKKFFQYYGYFTG"
BASE COUNT         3176 a         2200 c         1853 g         3096 t
ORIGIN      
        1 aatgtattta aaaatataaa caatttgtaa tttttaaatc attgtaactt gggttaagga
       61 gatttcaaag aatcatcctt gcttcctaca gtcagaattt tgacatactc tttatacttc
      121 accggatacc aatgtcgatt ggatttcttc tttgggataa attgggtgac aaacctaacc
      181 tggcttaaat agagaacaaa tcaaattttc ttttttacat ctttatctaa ttggcgcaga
      241 aaagcgagct tttctgcaat tttagtttct aaacctcttt gtaccggatt ataccaattt
      301 acttctggca ttccttcagg taaataacat tcaccagccg catagccgtg tggttcatca
      361 tgagcatatc gatattcatg tccataacct agttttttca ttaattgagt aggagcgttt
      421 ctcaagtgaa tgggtacttc tctggacttg tctcttttta taaattccat agcttgatta
      481 aatgcgacat atcccgcatt gctcttggca gcaacagaca aataaattac agcttgcgcc
      541 aaagcaagct cgccctcggg tgagccaagc ctctcataag ttgcagcagc atcattagcg
      601 atttgaatcg ctttcggatc tgctaaccca atgtcttccc atgacattct aataatacgt
      661 ctggctaaat aatatggatc cacaccacca tccagcattc gacatagcca atataatgcc
      721 gcatcaggat tagagcctcg taccgattta tgtaaagcag aaatctgatc gtaaaacgct
      781 tctccacctt tatcaaaacg tcgactattt tgtactatca cattatgcaa aagttctaca
      841 tcaacaactg ctgttttaat tgttaggcag gctgtttttg tttgctcaag taaattgagt
      901 aatctcctgg catccccatc agcacaagaa attaaaaaat caacggcttc ctcggtaaat
      961 tgcagagaag ataaagctct ttggtgagcc ctaagaaata agagttttaa ctcctgctcc
     1021 gataacggtt ttaaaacata aacttgtgcc cgtgataata acgctgaatt aacctcaaaa
     1081 gacggatttt ctgtagttgc cccaatgaaa gtaattaagc ctgattctgt ataaggcaat
     1141 aaggcatctt gctgagcttt attgaatcta tgaatttcat caataaataa gatagtctgc
     1201 ttatcatgaa ttaaatattc ctgcgctttc tcaaccgcag ctcgaatatc cttaactcct
     1261 gaaaatacag ctgataatgc tatccattca caatcaaaag cctgtgcagt taatcttgct
     1321 atagtagtct taccgacccc aggtggcccc cataagatca tagaatgcaa tttggagccc
     1381 ataaaacaaa gtcttagagg tttaccctct ccaagcaaat gactttgacc tatgacctca
     1441 tcgatatgct gaggccttaa actttcagcc aatggtggat caggttcttt attgaatagg
     1501 ctcataaatc aacattaaaa ttattgtttt accacatcca cacctttagg gggcttgaat
     1561 tggaacaatt tggcaggtaa cttaggattt gacttaatct gcactaattt aacatcagta
     1621 atttggccta gctgatcata taactccaga ccaatcaaag tactttgact aaaaattaac
     1681 ttaattcttt gaaaattttc ttttgcagat ttggatttca aatcaaaaac ggtcaatttc
     1741 cctttttgtt tttcactgac atcgaaatca tgagtaagag tttcatcata gccgctcaaa
     1801 aacaatgcag cagtaccacc tagtcccttt tcctgatttt tgactgtgac ttgttcaagg
     1861 tccacatcat aaatccacat tttttggccg tcagcaacaa ttaattgttc caatggatct
     1921 tttgtttgcc agcgaaatcg tccaggacgt tgcaatgcca tggaacctga agatcgtgac
     1981 acttcacgat tctttgcctt tacaatttga ctaaaatttg cagtcatggt ttgtatagca
     2041 tttaatttac tctgcagtac ttcggcagaa gtttgactaa acacttcatg cgaaaaaatg
     2101 agcaacaaaa tgagaaacaa tttattcata ttcagtcctc agtcactgat gttactaata
     2161 cgtcacgata accaccatcc aatggaccga ctattcctgt cctctccatt tcctctatca
     2221 ttctggcagc gcgattataa ccgattttta atcttctctg taccgcagaa atactagcct
     2281 tgcgtgtctg aattacaaat tctaccgcct gatcataaag aggatcatcg tcttctacgg
     2341 attgcccccc actatcatca tcgaaagcac catccccatt ttcatttccc attttaagga
     2401 tgtcatcaac ataatcaggt tcacctcttg accgccagtc atcagcgata cgatgaactt
     2461 ccttatcatc gacaaaagca ccatgcacac gcaaaggagc tccacttcct ggtgccagat
     2521 ataacatatc accatggccg agcaattgtt cggctccttg ttggtcaagt atggtgcgcg
     2581 aatcaatttt tgaagacacc tgaaatgata ttcttgttgg aatattggat ttaattaaac
     2641 ctgttaatac atccactgaa ggtctttgtg tcgcaagaat catatggatt cctgctgctc
     2701 ttgctttttg cgcaatacga gcaatcaatt gctccacttt cttgccaaca accatcatca
     2761 tgtcagcaag ttcatcaatt acaacaacta cataaggcag tgcttgcaat tctggtgcag
     2821 tttcatccat agaatcgacg ggtttccata atggatttaa caatggctga ccattagctg
     2881 ccgcttccgt aattttggtg ttataccctg ccaaattcct aacacccaac gcagccatta
     2941 acctataacg cctctccatt tcctctacac accagcgcag tgcgctcgct gcctccttca
     3001 tgtcggtaac aacaggagtc agcaaatgtg gaatcccatc ataaactgat aattccaaca
     3061 ttttgggatc gaccataatt aaacgcactt gctctggact tgctttaaaa agaatactta
     3121 aaatcatggc attaatacca actgatttac cagaaccagt tgttcctgca accagtagat
     3181 gaggcatttt tgccaaatca accaccatag ggtgcccgcc aatatcaact cccaaggcta
     3241 aagataaggg agaatgagct tgctgatata cgtcagcaga taaaacatct gataacctga
     3301 ccacttggcg tgaatgattg ggtaattcaa ggcccacaac agttttacca ggaataacct
     3361 ctacaactcg gacagaaata actgataacg aacgagccaa atcctttgcc aaagcggtca
     3421 gtttgctcac tttgacccct gctgccaatt gcaattcaaa tcgggttacc actggccccg
     3481 ggtgaacggc tacaacatct gcctgaatac caaaatcaag caagtgttgt tctacatctc
     3541 ttgaaagact ttccaattcc tcatgtgtat aacctcccat cggcttccct ggttggcctt
     3601 tatctaacaa cgacagactg ggaagagctc caggggttat tgttttagga ggacgaattt
     3661 ccttgaattc attggtaggt ttgacaattt caggtttttc ttcacttgca attaaaacag
     3721 gtgttgcttt ttcttgctct ttatctttct tgtcctggaa taatttagga acagatttct
     3781 ttttttcatt atcaggttta gggagttgtt cggtcttgat caatggtgtt tttaatttct
     3841 ctttgttaaa attttcagaa ataatttgca ttccttttct gatgaattta tcaagaaatg
     3901 tgaaaagatt taatgtgtaa catccgatta actcaattgc ctttatccag gataatccag
     3961 tcagccaggt tgttcccaca agaaacattg ctaataaaag taaagtggcg ccttccatgt
     4021 taagcatttg gtaccaacca tttccaactg tttgtccgat aatacctcct gaactgtgaa
     4081 tggtatctac tgcttcaaag cgatgattta tactcaataa accacaacca ccagaaacca
     4141 tgaggataaa accaaccgtt ctcagtaaca gaaccattct gtccaacgct ttaaaagagc
     4201 gaaaatcctt caaaatggcc caggcaatgt aaacaaatgc tactggtaat aagaaagcaa
     4261 aatatccaaa tgcaaaataa agcgcatcgg caatatacgc accaacttgt ccccctgagt
     4321 ttgaaacgga catacctgaa cgggaggcat gagaccaacc tggatcattg gttgaatagg
     4381 taaataaaga caagagaaca aaaagagctc ctgtcaaaat caatataaaa ctcccctcac
     4441 acaagcgttt tattataaag ctcggcatgg attttttagg gattccagcc tgttttcccg
     4501 aatgttgttt tgccataggt attttaatgt ttttattttt gtcataatat ttgccatctt
     4561 aacacaaaaa ataaggaagg cagagacaat gagcataagc aatcaccatc gcttaatcat
     4621 actcggttca ggtcctgcag gttacactgc tgcagtctat gccgcaagag ccaatctgaa
     4681 tcccgttttg atcaccggaa tgcagcctgg cggacaatta acaaccacga ctgatgtaga
     4741 caactggccc ggagatattg aaggattaca aggccccgcc ctaatggagc gtatgcaaaa
     4801 acacgcggaa cgctttgata ctcaagtcat attcgatcat attgtcaaag ctgatttagc
     4861 gcaagctccc tttacactac aaggggatag tactacttac acctgcgatg ccttgattat
     4921 tgcaaccgga gcctctgctc gttatttggg attaccctca gaaacggcct accaggggcg
     4981 aggtgtctca gcgtgtgcta cttgcgatgg atttttttat cgcaataaaa gtgtttgcgt
     5041 tattggagga ggcaatactg ccgttgaaga agcactttat ttatcaaatt tggcatcttc
     5101 cgttactctg attcacagac gagacagctt gcgtgcagaa aagatactgc aggataaact
     5161 ctttgagaaa gcgcattccg gtaacattaa aataatatgg aactcaaccc ttgatgaagt
     5221 actgggcgat gggaaaaaag tcacaggagc catgattcgt aaccttaaaa ctggtgaaac
     5281 tgatcaactg gcaatggatg gtatttttat agccattgga cacacaccaa atactgcaat
     5341 atttcaagat cagctggcta tgagagacgg ttatatcttg attaagtcag gacttgatgg
     5401 aatggcaaca agcacttctg ttcctggagt atttgcttgt ggcgacgttg ctgatcatgt
     5461 ttatcgtcaa gccattacat cagcaggatt tgggtgcatg gcagccctgg atgctgaaaa
     5521 gtacctggat tcaatataaa attggcttac gatagcaact atacttttcc tgacccggaa
     5581 accagtgata aacagggttt attagcaatt gggggtgttt tgacccccaa gcgagttttg
     5641 caagcctact cccaaggaat tttcccttgg tatgagcccg gtaaccctgt tctttggtgg
     5701 tcacctaatc cgcgcctgat cttaatacct aacgaattta aaatatccag aagtttaaaa
     5761 aaaactctga aaaaaccttt taaactgact gtcgatactg cttttcaacg agtcatttca
     5821 tactgtgcaa cttgttcaga caggtctaat aagacctgga tcactagtga aatgatagaa
     5881 acatacaccc aattacatga gatgggatat gcgcattcct ttgaaatctg ggatggttcc
     5941 gaacttgtag gcggactcta tggaatcagc cttggacacg ctttttttgg tgaatcgatg
     6001 ttccatacaa taacagatgc ctccaaagtc gccttgcatt ttttatgtcg cattatgcaa
     6061 tcatggaatt ttgattttat tgattgccag ttgccaacct tgcatttaat gagccttggc
     6121 gcaaaaataa taagtcgaaa agaatttctt catatgttac aagaaacttt aaaataccct
     6181 gacaaaaagg gaaattggtc tattgattaa attttttatc gataaccttt tatctttata
     6241 aaactaaata gagtgagaat aagcatgaag caacattcac aaggtttttt aaaactggta
     6301 acagaagcaa aaaaacgtat aaatgagata agtcctcaaa ttttaaaaag taaaatcgat
     6361 aacaatgaaa aattaatcat aatagatgtt cgtgaaacag atgaatggga aacaggtaaa
     6421 atcccgaacg caattcattt aagtaaaggg attattgagc gtgacattga aaaaattatc
     6481 acagacaatc aaagcaatat tgtagtttat tgcagcggag gttatcgctg tgctttggta
     6541 gccgatagtc tacaaaaaat gggatatacc aatgtattgt ctctggatac tggattgcaa
     6601 ggatggctag aggcgggata cagcttggta aaataattga atatctccca ataatgagcc
     6661 ttgagctata tctttgaaca atgtaattgc aagactggat tgccattttc cgcaatttag
     6721 tgcactattt cacttttaaa aatttgatac atcatttgag agacatatgg caaaagaaga
     6781 tcacattgaa atggctggta ctgttataga tacgctaccc aacaccatgt ttcgcgttga
     6841 gttggaaaat ggacatattg ttacggctca catttcaggt cggatgagga aaaattatat
     6901 cagaattctg actggggata aggttaaggt agagctgact ccttacgatc tttcaaaagg
     6961 tcgtatcatt tttcgcgata aaggctaaat gcgtttcatc caatttctat agctgttctc
     7021 tgtttcatta aagaaaatct gccggaatat agaaaaagct ggctgcaagc agccaggtat
     7081 atcgctaaaa atgttccaga gaactatcat caagattaca gagcaagtgg ttaacctcga
     7141 tggtttgggg cattttattt ccctgcaacc atcatatttt ctattaaaat agagccacag
     7201 cgcatggaaa tattcggatt aatgtcactg cctactgcca atatcatccg gtacatttct
     7261 ttaagatttc ctgcgatagt aatctgatca accggatatt gtatgacccc atcctcaacc
     7321 cagtatccac ttgccccacg tgaataatct ccggtaatac cattcacacc ctgacccatt
     7381 agttctgtaa ccagcaaccc tttccgcatc attttcaaaa ggtcaccaag atccccgcaa
     7441 gtaggatcaa tggttaaatt atgaacgcca tcactatttg ctgttgtcct taaacccatt
     7501 tttcgcgccg aataactgcc aagaacatat tgaagcaagc gccccttctc aactaataca
     7561 ttgggtcgcg taggcacacc ttccccatca aaaggggcgc tccccaaagc accttttaaa
     7621 tgtggctgtt cgtaaatatg aacaaactcc ggaaacaatt gttgtccaat ggaatcaagc
     7681 aaaaaggaat tttttctata taaatttgaa ccactaatag cgctcacaaa gctacccaat
     7741 aatccactgg aaactcttga agaaaaaata accggagtca tttgtgtttt aatttgtttt
     7801 gatcccagac gacttaatgc cctttcaaca gctttagctg ctatcaaatc agctgagact
     7861 aaattttctg catttctggc agtcgtataa tcgtagtctc tttgcatttc atcaccttct
     7921 ttggcgatta ccgagcaact tacgctatgt cgagtactat gaataaaccc ctctccacca
     7981 tgggtattgg cataaccgtg atgagattca taggaagaaa cattaacacc atctgaatta
     8041 gtaatccgtt tatctaaaga caaggcatgt gattcgcatt ttaaagccaa atctatagcg
     8101 tgttgtgggg taatgtccca ggaatgatac aaatcaagat ctggatgatt ctttgtcatc
     8161 aattccttat cagctaatcc aaagcaagga tcttcggcac taaccttcgc tatctcacag
     8221 gctgctttaa ccaaagattg taatgcttca ggagaggtat ctgtactgct tgctccccct
     8281 ttgcgctgac ctatgtatac ggttaaacca actcctttat cttcgctaaa agcgactgtc
     8341 tccacttccc ccattctcac atcgactgaa aaacctcgat cataatttac cgctactgct
     8401 gcatcagtgg caccttcttt tttggcaagt tctaaaacat cttttattac tcgatttaaa
     8461 tctgttgttg acttatatac ttcactattg ttattttgcg gtttaatttg catatgagat
     8521 tccgtggtgt tgaatgatgt acataaggat acaataacat gtcttcgcaa accaatgttt
     8581 atcctgcaaa caaattgaat ttatggctct tgcttgtttt agcactcgcc attgtcatcc
     8641 ctatgacttc ctacgctggc agtgattggc aagaactgac tccaggtatt gaatatcagg
     8701 atctcgaagg aggcttgtta aacccttggt ctcatattca tgtatttcgt atcgatctta
     8761 ataaaaatca gatggcactg gtgacagcca aaaaccttgc tcaaaaaaat gcatccgttg
     8821 atcagtttgc cgaacacagc aaagctttac ttagcataaa tggcgggttt tttgatcatg
     8881 aatttaatcc tctgggactt aggattaaca acaaaaaaca ggaaaatcca ctaaagcgca
     8941 ttagctggtg gggcattttt tatgttaagg ataataagcc tcgtattact aatatacgca
     9001 attttcatta tgacagtaac attgattttg ctattcaaag cggccccaga ttattaatca
     9061 gaggaaacat cccttctctt aaagccgggg ttgcagacag aacagcgtta gggatcacgg
     9121 acgatggcaa agtgattata ttagttacaa caaatgctgc catgtcaacc agacaattag
     9181 ctcaaattat gagagcacct ccattatcct gtagtgacgc aatcaatttg gatggtggaa
     9241 gttccagcca attatatgcc aagattgata gcttccaact caatgttcat ggtttttcta
     9301 atgtcagtga tgcgatcata gttaagaaaa tttagtatca ttcaaggcaa gccccttata
     9361 atcggttggg ttcaaactcc ttatagaatt aaattcacgc gctccaacca cctgcagaac
     9421 ccccttttca aattccacct tcgtttttgc gagatccgta atcccgaatc tgctatatca
     9481 ttcgtatgca taaatcacaa tcaagccatg atttttttaa tgatataata tagattattg
     9541 agataaaaat atttataaat tactccattc aacaggtaat ttaatgaaat taaaagaaat
     9601 cagaacaatc atctacaact atggcgagga agaaatcctt aagcatatct tgtatgaatc
     9661 caaagagcct atcttaatta aaatacctga ttttaccagc caattttcac tcgattattt
     9721 tgaacaactc actcaggcca aaactactta ttgtaccttt gaaaacaagc gatgtgttga
     9781 tttccaagcg ggcaattttg ctgaggtcat ccaacaaata aaaagcaaca aacccattcg
     9841 tatttttggg caaattttgt ctagagaacg ctctaaggaa atagaaaagc atgtgccact
     9901 ttggcaaaaa attcctttca gaccaaggta ttttagagag accaaaaaag tggcttattt
     9961 ctttggaggt aaaggcgctg tctcagaaat gcattttgac cgagaacatt gctgcaatct
    10021 ccatttatgt ttgagtggga aaaaacaagt attgctgttt accagaaaac aaagtgacaa
    10081 cctttacaaa ctccccttta tcggggattc tttaattgac ttcactcaac ccctggatgc
    10141 tttaattcaa caatacccca gactgaatca ggctgaagga tatcaggttc tacttgagaa
    10201 aggcgatatg ttattcatgc ctagaaattg ttggcattac acagagtatc ttgatgcgtc
    10261 atctgccgct acttatgttt tttatcctaa aaaatttttc caatattatg gatattttac
    10321 cggca
//
LOCUS       DATNSN010000120        10204 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.120, whole genome shotgun sequence.
ACCESSION   DATNSN010000120 DATNSN010000000
VERSION     DATNSN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.120"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            35..418
                     /locus_tag="VAH76_002329"
     CDS             35..418
                     /locus_tag="VAH76_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEO1386931.1"
                     /translation="MYIQKLKEMFAQMVIKKNVNLIPEYYHGEFLLYTNDIVTGYDEF
                     LSSHQQYYATEIQYQVEYDEETFLEQGEKIAGRVFITTTRPNEPAKRIEVILIAQYKD
                     EKIYRLWELTYPDWSKLPAFSNQLT"
     gene            643..1197
                     /locus_tag="VAH76_002330"
     CDS             643..1197
                     /locus_tag="VAH76_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEO1386932.1"
                     /translation="MKGIIFNEFLNFVEKSESYTLVDQIIMDSHLKSHGAYTSIGTYS
                     PKELFQLVKALAMKNGKPTSVILQEYGEYLFEVFAKKYPQFFREKKSVFQFLEALETH
                     IHFEVKKLYDHTELPHFECQYHSQNQMEMIYTSSRPLADFAEGLIRGCIKYHKENMTI
                     ARENLPAKTGFKVRFVLTKGDPDE"
     gene            1190..2467
                     /locus_tag="VAH76_002331"
     CDS             1190..2467
                     /locus_tag="VAH76_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEO1386933.1"
                     /translation="MSDYEVLLRHLDREIRARKEAEEISEKKISELYRFAQRLEESLK
                     AQKEISKKLKEKTKALKQQIAKTEEERHAKSTLQTILESAIEYSIIAINLKGVILVWN
                     EGARRNYGYEAQELVNRESIEILHTVEDIQSGKVRKFFQTVRREGKAEEIFQRVRKDG
                     SQFTASVTMTIHHDSQGKKVGYVIISKDITEIKEFEEKLIKSNQDLEEFSYIISHDLK
                     APLRAIIQLATWIEEDCGNKLDSLSRKNLQLLRERTRRMSGMVDGLLQYARAGRVNME
                     VEWVDTKELIQEIIDILNHDEQFTIIVHESMPQLPTAKLLLSQVFSNLIANSMKHHHC
                     KTGTIEIGVLDKGTFYEFFVADDGPGIEPVYFEKIFRIFQTLKSKDEAETTGIGLTIV
                     KKIVESQNGKIEVDSELGKGARFSFTWPKVPHQ"
     gene            2531..2938
                     /locus_tag="VAH76_002332"
     CDS             2531..2938
                     /locus_tag="VAH76_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1386934.1"
                     /translation="MDLAADKVDILYLEDDEVDIQSVERVFHKISSLIKIEIAKSGNQ
                     ALDMLYGRNKENKIHPKLILLDINIPKMNGIEFLKELRADSSFTDIEVFVLTAAYTSK
                     DKLAFEPLNIRGHLIKPLDYGEAIKLFWILQSM"
     gene            complement(2950..4371)
                     /gene="ankD"
                     /locus_tag="VAH76_002333"
     CDS             complement(2950..4371)
                     /gene="ankD"
                     /locus_tag="VAH76_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HEO1386935.1"
                     /translation="MLTPPPDSKITTTDKSLDKLSIPMDMLKQMNESTMEQTKLDELR
                     KKMSLQTEIINKAKADNDMFFRLLIELMSLKLQGELFKEQLSKISKESGYDSVQSALI
                     QATNSEGQSPLQYALQKQDFTTAKYFLDNGAKAGPIEKAVFEIALDSKAAKEFGFPPL
                     PPEKEKLHPVKNFGLVLGIKTTSVDGTPSQFGHIAPTYQLMTDSVSHFAKSHPGNKNF
                     QEIANAFHFSNEASAFKFSTPQRNPEAGQDLSRRIQSGELTTIPVSCKGHAMGLSYVP
                     DGPGSKSGYLVYTNRGLGAKSHEHGTHIFRIDDSSKITSEFINNMTNGHSNGASHDEI
                     MSQIKAAAGNKEPIHHIKQKGQKNDNCTIANSRSNIEGILLCQKARDVGGFDKLTESD
                     MDSVKKEYKEFTKHMRVEKVNELAKALKENPRDPDLNNLTKEYLKQHPKADPKLKETL
                     ETALKQASESSMELSQPGTYKTI"
     gene            complement(4578..5207)
                     /locus_tag="VAH76_002334"
     CDS             complement(4578..5207)
                     /locus_tag="VAH76_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386936.1"
                     /translation="MTKIVYLQNDPDKMLYYKDSVPKDLKVVEPDAKMMLEILEQKLK
                     LLEVVDLDWFYCAICMELPKEPVFYPTSEGITNLYEKSELVKWFSRDLKNKSDPLGLG
                     SNVTISQYKTPTPKELLDFVIKSTGFAEDVFIDHYKKPLAKKVSPEEELLLEKPDNTR
                     RNEIDSPSHSKQRSCLSLFLYSIFGCKDNRKITQEKPESLTSDYTLPHH"
     gene            complement(5368..5814)
                     /locus_tag="VAH76_002335"
     CDS             complement(5368..5814)
                     /locus_tag="VAH76_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386937.1"
                     /translation="MLHFKWCEFSELTSKQLYDILALRASVFVVEQHCPYLDPDGRDF
                     FSMHLLGLEENSLVAYLRLFLPTEIENYLTFGRVVTAKSARTKGYGRKLIRELLSYCD
                     MHYPGIRIQCSAQNYLKKFYEEFGFKSYGSVYEEDGIPHIAMQRTL"
     gene            6119..6568
                     /locus_tag="VAH76_002336"
     CDS             6119..6568
                     /locus_tag="VAH76_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386938.1"
                     /translation="MSFNNFLKTFNEFLLEQAGTTYRAVDHYLKGKDKTLKSVFFAPY
                     SSAPNFLYRAGHVITAPISFSIITLELVSSSLYLSLKSLNSLVFSDKKAAKIHIIDSV
                     VHFAVSLITAIGVIVSPIINLIDLIGGAISTMRVKSEPVEQMRPSVL"
     gene            complement(6616..7110)
                     /locus_tag="VAH76_002337"
     CDS             complement(6616..7110)
                     /locus_tag="VAH76_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386939.1"
                     /translation="MAFQRSFFSHIEDDWRYYQNIRAKYSDAIPIPQRKYFEPIHSID
                     SFATLTVRPIEKPLWLGVHTAGFLLKAIIHLVGALVLSPFALIFAVCVPRSELREQTV
                     SSFKSTAAGSIVAAGMACVALLSTLMSLIFNPLYALSRSAATGIDHLNSVTESCCGLT
                     IAKI"
     gene            7334..10099
                     /gene="ankF"
                     /locus_tag="VAH76_002338"
     CDS             7334..10099
                     /gene="ankF"
                     /locus_tag="VAH76_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkF/LegA14/Ceg31"
                     /protein_id="HEO1386940.1"
                     /translation="MKDQLAKIFEKYPFVHLDKSGELSIIVPLTGAASISDNFTADET
                     KPIFLYLFGDNTCKSDLERRNFFYGKQKSGGRHSVKPFTELFSNLIHELNGLKSSTEI
                     RVDEQVFLETIIANLNELKEQALTFRKGNPGFEDMKNDFSHSDEIMVSKSEFVVTVYP
                     DRVRQFDLPNVMSLINPGESRYKQLLAQSNINQCLEALSLVQKHAAEGNLDLIADALG
                     KLGADPIDYLCKKDNKDPKALFKAVYEILQKQDTSLENQQSAVFEVMESNLVAAFGDN
                     EDFSTWQKEEIVDAIKSIFKQANLLSEQSGTRQKTFADVYNQAFDKYNESTNDDSESG
                     CKRRNTYQLFILQTFLYLCSLQLRLKNKEKAKKFLGFFEDKGTLNNLVLQLCTDEQAF
                     MRMMADEFELSSAEYASIAESTYGIVLAHIDAEHFDELRIACSAQLLQDSNYIVMGGR
                     LCWSTSSINEEGNIRSLEQQKKISSENFEIFYNQRQSYIDKSREFQQIKVLLDKDVND
                     EGIAEAVASKVRYLTKYQKSQILSEVIQKKKYEHAKLLIENYANLVFPSVFISVIKST
                     PVHIDLIRAFLNADSRHALNIDGNDLIQAAKKGDFELVKIIIEHRPDLLESKDSFQQT
                     PLLWACANGYVDIVEYLMDSGADIHVRTKVSGSHRYKIGETPTGGRTALDWARCYDKT
                     DPVKASKIIALFDTYYQRMEDHFKSNIAYKDQFNKGHLTQAINNGDLRLIALFLKYCP
                     HLEKLFTQEEQLRAKQRHNQQILRSQIMPYQNSFNKLLDELQRKAELLNKKYGTLHEA
                     TNVMQALINDLSTVGNDFFMDDITPQSFQKFERKCKQVLEEASPVLSTHRGWHGYPDF
                     VRRIIGVIATLAVIPALVVHFSSAKGYVGVFFSGKENIKTDSSEKMGEFANNLETLKT
                     EISNKLS"
BASE COUNT         3298 a         1722 c         2023 g         3161 t
ORIGIN      
        1 gtataatttt gaaaaattaa tttgagagta gaaaatgtat atacaaaaat taaaagaaat
       61 gtttgctcaa atggtaatta agaaaaacgt caatcttatt ccagaatatt accatgggga
      121 gtttctgctc tataccaatg atatagtgac aggctatgat gaatttctaa gctcgcatca
      181 acaatattat gcgactgaaa ttcaatatca agttgaatac gatgaagaga ctttccttga
      241 gcaaggtgaa aaaatagctg ggcgggtatt tattaccacc actcgaccta atgagccagc
      301 caagagaatc gaagtcattt tgattgccca atataaagat gaaaagattt atcgattatg
      361 ggagttaact tatccagatt ggtccaagtt acctgccttt tcaaatcagc taacctagac
      421 gattctcttc tcctccaaac aggggaatat cagaaaagta gttggcgaag atttcccgct
      481 ttacatagtg tagcgccacg caggagctag caaggcttca tggtttaatc aatatgagca
      541 aaaaaatgca aaacattcct gcttgcgtca ggattatatt attgataaca aatttacgac
      601 tatacttata gtattaatca attgatataa aacatcttgt ctatgaaagg aatcatattc
      661 aacgaatttc tcaattttgt agaaaaaagt gaatcctaca ccctggtaga tcaaattatt
      721 atggatagtc atttgaagtc ccatggtgcc tacacgtcta tcggtacata ctctcccaaa
      781 gaattatttc aattggttaa agcgcttgct atgaaaaatg gcaaaccaac atcagtgatt
      841 ttacaagaat atggtgagta tttgtttgag gtttttgcaa aaaaatatcc tcaatttttc
      901 agggaaaaaa agtcggtgtt tcaatttttg gaagcgcttg aaacacatat tcatttcgaa
      961 gtgaaaaaat tgtatgacca tactgaacta ccccattttg aatgccaata tcacagtcaa
     1021 aatcaaatgg aaatgattta cacttcttcg cgtcctttgg ccgattttgc ggaaggttta
     1081 ataagaggtt gtattaaata tcataaagaa aacatgacta ttgctcgtga aaatctgcct
     1141 gcaaaaacag gctttaaggt aagatttgta ttaacaaaag gcgatcctga tgagtgatta
     1201 tgaagtattg ttacgccatc ttgatagaga gatacgcgca aggaaagaag ctgaagaaat
     1261 aagtgaaaaa aaaatatccg agttataccg ttttgcccaa cgtctggaag agagtttaaa
     1321 ggctcaaaaa gaaataagta aaaaattaaa agagaagacc aaggcattaa agcagcaaat
     1381 tgctaaaaca gaagaagaac ggcatgccaa aagtacttta caaaccatat tggaaagcgc
     1441 aattgaatat tcaatcatcg caatcaattt gaaaggagtc atcttggttt ggaatgaagg
     1501 ggccagacga aattacggct acgaggctca agaattagtg aacagagaga gcattgagat
     1561 tttgcataca gtggaagata tccagtcagg taaagtaaga aagttttttc aaacagtacg
     1621 tcgggaagga aaggcagaag aaatatttca aagagtcaga aaagatgggt cccagttcac
     1681 tgcttccgtt actatgacta tccaccatga ttcgcaagga aaaaaagtag ggtacgtcat
     1741 tatttcaaaa gatattacag aaattaaaga atttgaagaa aagctgatta aaagtaacca
     1801 ggatcttgag gagttttcat acattatttc ccatgaccta aaagcgccgc taagggcaat
     1861 tattcaattg gcaacatgga ttgaagagga ttgcggaaat aagttggatt ctctttccag
     1921 aaaaaatctt caattgctca gagaacgtac ccgtcgcatg agcgggatgg tagatggctt
     1981 attacaatat gcgcgggcag gcagagtcaa tatggaggta gaatgggtag ataccaagga
     2041 gttaattcaa gaaataatag atattcttaa ccatgatgaa caatttacta ttatagtcca
     2101 tgaatccatg ccccaactgc caacggccaa attacttcta agtcaggttt tttctaatct
     2161 gatagcaaat agcatgaaac accatcattg taaaacggga acaattgaaa ttggtgtttt
     2221 ggataaaggc acattttatg agttttttgt ggcagatgat ggtccgggta ttgagccagt
     2281 atattttgag aaaatattca gaatatttca aaccttaaaa tccaaagatg aggcagaaac
     2341 taccggcata ggattaacta tagtaaagaa aattgtagaa tctcaaaatg ggaaaattga
     2401 ggtggactct gagttgggta aaggcgcaag attttcattt acatggccta aagtccctca
     2461 tcaataaagg tttaggtcta taattaattt agaaataaaa aaataaaaca ttgtctcacg
     2521 gagaatagtg atggacctcg cagctgataa agtagatatc ctgtatttgg aagatgatga
     2581 ggttgacatt cagtctgttg agcgtgtatt ccataaaatc agttcattaa tcaagattga
     2641 aatagctaaa agtgggaatc aggcattgga tatgctatat ggtcgaaata aagaaaataa
     2701 aattcatccc aagttaattc ttctcgacat caatatacct aaaatgaacg gtattgagtt
     2761 cttaaaagaa ttaagagccg actcttcttt tacggatata gaggtatttg tacttacggc
     2821 agcttatacc agcaaggata agctggcttt tgagccttta aatattcgag gtcatttgat
     2881 taaaccgctt gattatggcg aggctattaa gctattttgg atattacaat ccatgtagac
     2941 agtgtggtat taaatggttt tgtaagtacc tggttgactt aattccatac tgctttcaga
     3001 agcctgtttt aatgctgttt ctaatgtttc ttttaattta gggtcggctt ttggatgttg
     3061 ttttaaatat tctttggtca aattatttaa atcaggatca cgaggatttt ctttaagagc
     3121 tttggctaat tcattgactt tttcaactct catatgtttg gtaaattcct tgtactcttt
     3181 tttaacggag tccatatcgc tttctgttaa tttatcgaac cctcccacat ctctggcttt
     3241 ttggcataat agtatcccct ctatgttgga tcgggaattg gctattgtgc agttgtcatt
     3301 tttttgtcct ttttgtttga tatggtgaat gggctctttg tttccggcag ctgctttaat
     3361 ttgagacata atttcatcat gagaagcgcc attcgaatgc ccattggtca tgttattgat
     3421 aaattccgaa gtgattttac tggaatcatc gattcggaaa atatgggtgc catgctcatg
     3481 actctttgct cccagaccgc gattggtata taccaggtag ccagatttgg agccagggcc
     3541 atcgggtaca taagacaatc ccatagcatg gcctttacag ctgacaggaa ttgtggtgag
     3601 ttcgccactt tggatgcgtc ttgaaagatc ctgacctgct tctgggtttc tttgcggtgt
     3661 gctgaatttg aaagcagatg cctcgtttga aaaatggaag gcattcgcta tttcctgaaa
     3721 attcttgttg cctggatgcg atttggcaaa atgactgaca gaatctgtca tcaattggta
     3781 ggtaggtgct atatggccga attgagaggg agtgccatcc acactggttg tttttatgcc
     3841 taaaaccaaa ccaaaatttt tgacagggtg cagtttttct ttttcaggtg ggagtggcgg
     3901 gaagccaaat tctttagctg ccttactgtc aagggctatt tcgaagacgg ctttttctat
     3961 cgggcctgct ttggctccgt tatccagaaa atattttgcc gtagtgaagt cttgtttttg
     4021 cagcgcgtat tgtaaagggc tttgaccttc tgagtttgta gcttgaatta atgcgctttg
     4081 tacgctgtca taaccacttt ctttggaaat tttgcttaat tgttctttaa ataattcacc
     4141 ttggagtttt aatgacatta attcgataag taaacgaaaa aacatatcat tgtctgcttt
     4201 cgctttattg attatttcgg tttgtagaga catttttttt cgtagttcat cgagtttggt
     4261 ttgctccatt gttgactcat tcatttgttt aagcatatcc attggaatac ttaatttatc
     4321 caaactttta tcggtggttg taatttttga gtcaggtgga ggagttaaca tggaacaccc
     4381 tgattggttg aactatatga ttattataca tcaggactta cgaaaaaccc caatctcttt
     4441 gctaaaaaat gttattaatt cagtgattca gtttatcgca aagctctcat taagcccatt
     4501 gtttgccctg gccttgaggt ggagcgtaag ttcagtatat aaccaaaaac tgtctaaaaa
     4561 gacttgattt gtttttatta atgatggggt agtgtgtaat ccgatgtcag ggattcgggt
     4621 ttttcctgcg ttatttttct gttatcttta caaccaaaaa tactatacaa aaagagactc
     4681 aaacaggatc tttgctttga atgagagggg ctatctattt catttcgtct ggtattatcg
     4741 ggtttctcaa gtaagagctc ttcctccggg gaaacttttt ttgccagtgg ttttttgtaa
     4801 tggtcaataa acacatcttc agcaaatcct gttgatttaa taacaaagtc aagcaattct
     4861 tttggagtag gcgttttata ttgagatata gtaacatttg aaccaagtcc aaggggatcg
     4921 gatttattct tgagatctct tgaaaaccat ttgaccaact cacttttttc gtaaagattg
     4981 gtaatgcctt ctgaggttgg ataaaatact ggctctttag gaagttccat acaaattgca
     5041 caataaaacc aatccaaatc aactacttcg agcagtttta atttttgctc taaaatttct
     5101 aacatcattt tggcatctgg ctcaaccact tttaaatctt tggggacgct gtccttgtaa
     5161 tagagcattt tatccgggtc attctgtaga taaacaattt tcgtcataac atctccaaag
     5221 aataatactt taaataacgt gagttatgga taaagatgat taataagtaa acatgataaa
     5281 aagtgtgctt tattgtggca ctaatgtcaa tttaaaattt gatcgggctg gtagatttac
     5341 aggaaacccg gattggttat tgcgttttta taaggttctt tgcatggcaa tatggggaat
     5401 accatcctcc tcataaaccg agccataact tttaaagccg aattcttcat agaatttttt
     5461 caagtaattt tgggcagagc attgaatgcg aataccaggg taatgcatgt cgcaataaga
     5521 caataattct cgtatcagtt tccttccata tcctttagtt cgcgctgatt tagctgtaac
     5581 aacacggcca aatgtgagat agttttcgat ctcagttgga agaaacaggc gcaaataagc
     5641 cacgagagaa ttttcttcca ggcccaataa atgcatggaa aaaaaatctc taccgtctgg
     5701 atcaaggtag gggcagtgct gttctactac aaaaacgctc gctcgtaatg ccagaatgtc
     5761 atagagttgt ttgcttgtta actctgaaaa ctcacaccat ttaaaatgaa gcatgaaaca
     5821 ctcccaatcc ggctaaaatc ttcgatgcat tatgctgaag ttatgactct gaaatcaagt
     5881 ggagtaatga tcaggatttt gttaaaaagt gtttttaatt aagtcaatta gtttaaataa
     5941 atccccttat taaaaacatt ttttgctgcc aactggatgt atttggcaag ttctgattat
     6001 accagggcaa atttacaggc aatggattga agtaaaaatc attatcgaaa ttatctttag
     6061 taattaatgc aaattcatat attatggtga ggattttttt aacagggagt atctatcgat
     6121 gtcgtttaat aatttcttaa aaacattcaa tgaattttta ttagaacaag ccggcaccac
     6181 ttaccgggct gttgaccatt atttgaaagg gaaagacaaa accctcaaat cagttttttt
     6241 tgctccttat tcatctgccc ctaatttttt atacagagca ggccatgtga taacggcacc
     6301 aattagtttt tctattataa ctttagaact tgtttcatcg tccctctatt tgagcttaaa
     6361 gtctctcaac agcctggttt tttctgataa aaaagccgca aagattcata ttattgatag
     6421 cgtggtgcat ttcgctgttt ctcttattac cgcaattggt gtgattgtga gccccattat
     6481 taatttgatt gatttgattg gaggagcaat atcaactatg agggtaaaaa gtgagccagt
     6541 agaacagatg agaccatctg ttctctagtt ttcgccagat ttgaaataaa taagggttgc
     6601 gattgaacca cctaatcaga tttttgctat agtcagaccg caacaggatt cggttactga
     6661 attaagatga tcgattcctg tggcggcact tcgacttaat gcatacaacg gattaaaaat
     6721 aagcgacatt aaagttgaga gtaatgcaac acaagccata cctgcagcaa caatagatcc
     6781 ggcagcagta gatttaaatg atgagacagt ttgctctctc aattcagatc tcggaacaca
     6841 taccgcaaag atcaaagcaa atggtgaaag aactaatgcc cctactaagt ggattatcgc
     6901 tttcagcaaa aaaccagccg tgtgaacacc aagccaaaga ggtttttcaa taggccgtac
     6961 tgttaatgtg gcgaatgaat ctatggaatg aatgggttcg aaatattttc tttgtggtat
     7021 gggtatagca tcagagtatt ttgcacgaat gttttgataa taacgccaat cgtcttcgat
     7081 atgagaaaaa aatgatcttt gaaaagccac tggaaactcc ttgttattga atcagcggat
     7141 attaaaaaaa ttggcagtgt ttgtaaataa taggcaagtt cttcattttt caaatcttgg
     7201 gattgtcttg ctgacagaga caacaagagg tttttactgg gcttatttat gcccacagtg
     7261 tcattaattt tgtcttaagc aaactatatt acaatggtgc caaaaaagct taacatgaaa
     7321 gagtataacg aaattgaaag accaattagc caaaatattc gaaaaatacc cctttgttca
     7381 tttagataaa tcaggtgagc tatcaataat tgtgccttta acaggcgcgg ccagtatttc
     7441 cgacaacttt actgccgatg aaaccaagcc aatttttctt tatttatttg gcgataatac
     7501 gtgcaaatca gatctggaaa gaagaaattt tttttatggt aagcagaaat ccggaggtcg
     7561 tcactctgta aaacccttta ccgaattgtt ctcaaatctg attcatgaat taaatggtct
     7621 caaatcgtct actgaaatcc gtgtggatga gcaagtattt cttgaaacaa tcatagcaaa
     7681 ccttaatgag ttgaaagagc aagctctcac tttcagaaaa ggaaatccag gctttgaaga
     7741 catgaaaaat gattttagtc acagcgatga aatcatggta tcaaagagcg agtttgttgt
     7801 aacggtgtat cctgacagag tccgacaatt tgatttgcca aacgtaatga gtcttataaa
     7861 ccccggggaa tcccgctaca agcaattact tgcccagtcc aacattaatc aatgcctgga
     7921 agcattgtct cttgtgcaaa agcatgctgc agagggtaac ttggacctga ttgctgatgc
     7981 gctgggaaaa ctgggtgcag accccatcga ttacttatgc aaaaaagata ataaagatcc
     8041 taaagcttta tttaaagcgg tttatgaaat tctacaaaaa caggatacct ctttagaaaa
     8101 tcaacaatca gctgttttcg aagtcatgga aagcaatttg gtcgctgcct ttggagacaa
     8161 tgaagacttt agtacctggc aaaaggaaga gatagtcgat gccattaaat caatatttaa
     8221 acaagcgaat ttgctgagtg aacaatcagg aaccaggcaa aaaacatttg cagatgtcta
     8281 caatcaggct tttgataaat ataacgaaag cactaatgat gattctgaat caggatgtaa
     8341 acgcaggaac acttatcagt tgtttatttt acaaacgttt ctctatttgt gttctttgca
     8401 gttgcgttta aaaaacaaag aaaaggcaaa aaaattcctg ggtttttttg aagacaaggg
     8461 gacgttgaat aatttggtat tacaattatg tactgatgaa caagcgttta tgagaatgat
     8521 ggccgatgaa tttgagctgt cttctgcaga atacgcgtct attgctgaat caacttatgg
     8581 catcgtcttg gctcatattg atgctgaaca ttttgatgaa ctaagaatag catgcagtgc
     8641 tcaattgctc caggattcca attatatcgt gatgggagga cgtctttgtt ggagcaccag
     8701 ttctattaat gaagagggaa atatacgaag tttggagcag caaaagaaaa tttcatctga
     8761 aaattttgaa attttttata atcaaagaca atcttatatt gataaatcaa gagagtttca
     8821 acagataaaa gttctgctag ataaagatgt gaatgacgaa gggatagctg aagctgtggc
     8881 gtcaaaagtt cgctacctga cgaaatatca aaagtcacaa atcttaagcg aagtgattca
     8941 aaaaaagaaa tacgaacatg caaaactttt aattgaaaac tatgccaacc ttgttttccc
     9001 atccgtgttt atttcagtga taaagagtac accggttcat attgatttga ttcgagcgtt
     9061 tctcaacgca gactctcgtc atgcgttgaa tattgacggg aatgatttaa ttcaggcagc
     9121 aaaaaaagga gattttgagc tggttaaaat cattatagaa catagaccgg atttattgga
     9181 atccaaagac tctttccaac aaactccgtt gctttgggct tgtgccaatg gctatgtgga
     9241 tatcgtggaa tatttaatgg attcaggtgc tgatattcat gtcagaacaa aagtgtctgg
     9301 gagtcatcga tataaaattg gagaaacccc aactggggga cgaaccgcac ttgattgggc
     9361 gcgatgctat gataaaaccg atcctgtaaa ggcatcaaag ataattgcct tatttgatac
     9421 ttattatcag cgcatggaag accattttaa atccaacatt gcttataaag accaattcaa
     9481 caagggtcat ttaacccagg cgataaataa tggggattta agattaatcg cattgttttt
     9541 aaagtattgc ccacacctcg aaaaactttt tactcaagaa gagcaactac gtgcaaaaca
     9601 acgacataat caacagatat tgcgttctca aattatgccg tatcaaaatt cattcaataa
     9661 attattagat gaattacaaa gaaaagcaga attactgaat aaaaaatatg gtactcttca
     9721 cgaagcaacc aatgtcatgc aagcactaat taatgactta tccacagtag gaaatgattt
     9781 tttcatggat gatatcacgc ctcaatcctt tcaaaaattt gaaaggaagt gtaaacaggt
     9841 tcttgaggag gccagcccag ttttatcaac gcatcgtggt tggcatggtt atcctgattt
     9901 tgtcagaaga attataggag ttattgcaac cttggccgtt attcctgcat tggttgtaca
     9961 cttttcatcc gcaaagggat atgtaggagt tttcttttct ggcaaagaaa atataaaaac
    10021 agattcttct gaaaaaatgg gtgaatttgc gaataacctg gaaaccctaa aaacagaaat
    10081 atccaataag ttgtcttagc tggagaattt ggctgctgaa gccaaatgtt tcgctccatt
    10141 ctctgtttgg gttgcagata aagggttcta tggagccctt gatttttgtt ctaattaaac
    10201 actt
//
LOCUS       DATNSN010000121        10179 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.121, whole genome shotgun sequence.
ACCESSION   DATNSN010000121 DATNSN010000000
VERSION     DATNSN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.121"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            25..2616
                     /gene="pepN"
                     /locus_tag="VAH76_002339"
     CDS             25..2616
                     /gene="pepN"
                     /locus_tag="VAH76_002339"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEO1386941.1"
                     /translation="MPDTTIYLKNYQPPSFAVETVELNFDLYDDHALVNSTLKLTRQK
                     DGALHLYGEELELIALHLNGRPLEHGKYVLTKDALIIEQCPDDIVLNIVTRIKPQENT
                     QLSGLYRSNHLFCTQCEAEGFRRITYYLDRPDVLATFTTRITADKKQYPILLSNGNLM
                     DTGETADGRHWVIWEDPFKKPSYLFALVAGNLACVRDSFVTCSGRTVDLRIYVEPGNE
                     DKCSHAMESLKKAMKWDEEVYGREYDLDIYMIVAVSDFNMGAMENKGLNIFNSKYILA
                     RPETATDQDFADVEGVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSRDMNS
                     RDVNRIIDVKVLRSAQFPEDAGSMAHPVRPESYQEINNFYTATVYNKGAEVIRMQHTL
                     LGKERFRRGMDLYFKRHDGHAVTIDDFVAAMEDANGVDLTQFKLWYSQAGTPEVRVLS
                     HFSEGRLEITMQQNCPPTPECHEKKPFHIPVRIALFDLKGEMIPIENELLELKEKEQR
                     FIFSGLNEKPVVSLLREFSAPVKIYDDLDQDDLLFLLRYETDGYAKWDAAQRLVLNCL
                     NENLKLPASEWQVSKTLVAAFQHVLLDDSLDMDLRAELLTPPGFEEVAATMKWVDVSK
                     VESVRDYFRQQLGLGLYTEASDLYKELWEVEDHRMHGQAYGRRKLRNVCLWLMMKAKE
                     SDALELCREQFKASRTMTDQIASCSLLVNCSRESHRNEAIEGFYKQWSHNELVLDKWF
                     AMQAACELPGTLDHVKKLTQHPAFCIKNPNKVRALVGAFCMANPRNFHALDGSGYVFL
                     SEVLIKLDRLNPQIAARLATPFTRWRSYDEPRQKLIQNQLDQLTKLDLSRDLREVVDK
                     SLIVEEK"
     gene            2840..3637
                     /gene="aroE"
                     /locus_tag="VAH76_002340"
     CDS             2840..3637
                     /gene="aroE"
                     /locus_tag="VAH76_002340"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO1386942.1"
                     /translation="MLRRFAVIGNPIAHSLSPVIHQMFAQQTQIELIYEKILGDDVKF
                     EQQISDFFIQYGNGLNVTLPYKKRAYELAKIRTQRCALAGVANTLWMEENQLHADNTD
                     GIGLIRDLSRFLELKDKKILILGAGGAARGIIFPLLEAKPLKLIVANRTLEKAEELKR
                     QFPQINVTSFAELPEFFDLIINATSASLSDQVIALPEEAFSHKPFCYDLAYNQKTSTA
                     FVQYARNGGCEAVDGLGMLVEQAAEAFFIWNKVMPSTQKILEHLRSF"
     gene            complement(3687..4799)
                     /locus_tag="VAH76_002341"
     CDS             complement(3687..4799)
                     /locus_tag="VAH76_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEO1386943.1"
                     /translation="MMVKKALYLAGGGARGAYQAGVLKAIGHILQTKTLPFDLVSGVS
                     VGSVNAAVLAENANDFPAALEKLEAIWGEIHCQQIYKASNYELSKSVMRNLSTLIIKQ
                     RQSGHLLDTTPLRQFLEESIDFTRIEQNIQEGYLDTFEVLSACYETHQTISFYAHNHS
                     EFEDWNYPRHSSQRAVINAQHILASSALPLFFPPVSLNGFHYGDGSIGLVAPLRGAIR
                     FQVDRILILGTRELPEFTDPETLRNGEIAFAHILGNMMNGLFLDNLDRDIEMVNRMNE
                     IATLLSMWKKRRSPWRPISTLHLRPSRAMASVAQSHYTSMPVLLRFLLNIMGARSHSG
                     DLLSFLLFEKEFTRELLELGYQDTIAAATEVSAFFS"
     gene            4937..6319
                     /locus_tag="VAH76_002342"
     CDS             4937..6319
                     /locus_tag="VAH76_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386944.1"
                     /translation="MGITKDERFKKFEIEKTLQLLQSMQRPVEYPLTPTEKTSLLKKT
                     TKRIPLLSKFLKTVDDTGEVVVKIGDRMTSLSHTPGKESLAHGFAFGGVAMAGFDFLR
                     IPIIYLSAYLLGEEVPVNLSNNAKWFYSALLLGLTITALAVPVTAPYIAFATAGLSLG
                     VGTFLLGKTLYERYQLGRERRKLKAEIIREEDEMLKIQDKAKHLESLLQSADNEEQIA
                     AILYEISVVQEDYNRQKKHIQSLKNDELQLEQQIKNLGTMKVVDRTVAVALSALTLAA
                     LAVTLFFPPVGGGMLAGIAIFGGAYFVARVSAPLIKATAVWLYNKLKSTPSESPESQD
                     IDNKPTLSNEKEEKKEDSLQIDNEFVLPPIDAETLISEDSKEYKPVHESTEDVLVGLS
                     VNIQGLKEKARAEKKEEIEEALPEEPQHFSSLSPSPRLSGPRKLSAKESKDEDGESES
                     DIEHPDVPKI"
     gene            6387..7889
                     /locus_tag="VAH76_002343"
     CDS             6387..7889
                     /locus_tag="VAH76_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1386945.1"
                     /translation="MLNLFREQPRAFHMIFMLELWERFGFYTVQGILTLYFIRYLGYS
                     DLESYYTFGAYSALVYGMVVIGGYLGDRILGTKRTIVLGLVVLAMGYFSLAVTDKQHV
                     FLALGLICVGNGLFKANPSNLLSKCYDDNDPRLHGGFTLYYMAINLGSMVALFVGPAV
                     SSAYGYSYAYMISAIGLILGIVNYWYQRQHVAHVNTKADLRKITAFHWGTIIAGVLIL
                     TGISAYLLQHVMLAKNLLWGITLLVVSVYIYLMRKQAQASFMRMLVALVLMIEAVIFF
                     TLYQQMPTSLNLFAVNNVIPTFLGITIDPQSFQALNPIWIVLMSPVLAYFYRKLHQHN
                     ILFSIPYKFAFGMTMCAVSFLMLYFARYFYVGNGMVSSWWMIFSYLFQSLGELLVSAL
                     GVAMVAELVPAANAGFVMGMWFLTSAVAGFIGATVASFTALPENIKPGVESLLIYSDV
                     FACIGLVTLLIAVIMWLVSPRLSRYITVPSLERDKDIETVEYSTTYIPSH"
     gene            8150..9463
                     /gene="lem29"
                     /locus_tag="VAH76_002344"
     CDS             8150..9463
                     /gene="lem29"
                     /locus_tag="VAH76_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem29"
                     /protein_id="HEO1386946.1"
                     /translation="MGLEKKIKLSQRSLELLNDYLNLGGSGPSNLVNESSSIDFSSLT
                     QLLASNLSKSSKTSKTIKKKREFIEAALYAELLRDLSTSLNVSDEEIKPAEKEPPVSN
                     KLKFWLLAIAGTLLAACEGFDSITTMMAVLSFPAWIVLCAGILFSALSVVVFYGFDLV
                     QVSQNLEIKLTDTPKLLDLYLSQMEEIKAIRKKIDNYSLASMSMDELNELHLILVMLQ
                     DRVNSLVKAGKKFDLALNSPKMDVAKTIVSGVSGILFFGGGFFAGQSVATFMLALVMT
                     SVTPTFWPVLLFSVIVGLAAFSLYWYVQRVGVNKLVSEWFGLDEDKIEKLCGKSKMDK
                     ELKKLNNLDEKVMTTSDLLSKVANLESKLIDIGKQMKEMSAHQAPEKKLEGPAGLKAS
                     SNVYSFHFNSSNRDQESVYGERVLDDAVSDYGRGQNHNDSNHSLC"
     gene            complement(9348..10148)
                     /locus_tag="VAH76_002345"
     CDS             complement(9348..10148)
                     /locus_tag="VAH76_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386947.1"
                     /translation="MNRHDVKAIHSYVVDEEKKKISLKLINKLYRFLSKYYHLASQEE
                     FIRFIIQPDLYGELTVLYGENKEIAGFSRNCRQSVDLGKKQVTCYIAYLYLNPEYQIH
                     ASIKSAGLTQAIKYKLANPHEELVYIAFANNPLTYEFIYQLSDLIYPKPLQRVPDQIL
                     TVLNALKKQNGWISTNNHPMVVNSPLVPLRSQLSRINEESSELKEFYMSANPDYLQGN
                     SLLIYMPLHLANIGYGLNHYDSDPYHNQKQHHQGPSHRKRSPDPDLRN"
BASE COUNT         2979 a         1763 c         2224 g         3213 t
ORIGIN      
        1 ttttttttat aggaagcaat gactatgcca gacacgacca tttatttgaa gaattatcaa
       61 ccgccttctt ttgcagttga gactgtggaa ttgaattttg atttgtatga tgaccatgct
      121 ttagtgaaca gcactcttaa gttaacacgt caaaaggatg gcgctttgca tttgtatggc
      181 gaagagctgg aattaattgc tttgcattta aatgggcgac cattagaaca tgggaagtat
      241 gtgttgacga aagacgccct tattatagag caatgcccgg atgacatagt acttaacatt
      301 gtgactcgta ttaaacctca agaaaatacg caattatcag gactttatcg ttctaaccat
      361 ttattctgta ctcaatgcga ggcagaaggt tttagacgaa ttacctatta tcttgataga
      421 cccgatgttt tggcaacttt tactacacga attacagcag ataaaaagca atatccgatt
      481 ttattgtcta acggtaattt aatggatacc ggagaaaccg cggatggacg tcattgggtg
      541 atttgggagg atcctttcaa gaagccctct tatttattcg ctttggtagc gggtaattta
      601 gcttgcgtta gagatagttt cgttacttgc tccggcagga ctgttgattt acggatttat
      661 gtagaaccgg ggaatgaaga caagtgctct catgccatgg agtcactaaa aaaagccatg
      721 aaatgggatg aagaggttta tggccgtgag tacgatttag atatttatat gatagttgcg
      781 gtgagcgatt tcaatatggg agcaatggaa aacaaaggat tgaatatatt taattccaaa
      841 tacatcctcg ctcgccctga aaccgccact gatcaagatt tcgcggatgt tgaaggtgta
      901 gtaggccatg agtatttcca taactggaca ggaaatcgtg tgacttgtcg cgactggttc
      961 cagttaagtt taaaagaggg attgactgta ttccgggatc aggagttttc tcgggatatg
     1021 aattctcgtg atgtcaatcg gattatcgac gttaaagtat tgcgcagtgc tcagtttccc
     1081 gaggacgccg ggagtatggc gcatccagta aggccggaat cctatcagga aattaataat
     1141 ttttatacgg cgacagtata taacaaaggc gcggaagtga ttcgaatgca acatactttg
     1201 ttaggcaagg agaggtttcg acgaggcatg gatctttatt tcaaacgcca tgacggccat
     1261 gccgtaacca ttgatgattt tgttgctgcc atggaagatg ccaacggagt tgacttaacg
     1321 caattcaaac tctggtatag ccaggcaggg acacctgaag tgcgcgttct cagccatttt
     1381 tcagaaggac gattggaaat taccatgcaa caaaattgtc ctcctacgcc tgagtgccat
     1441 gagaaaaagc catttcatat cccggttcgc attgcattat ttgatttgaa aggggaaatg
     1501 attcccatag aaaacgagct tctggagtta aaggaaaaag agcaacgttt tattttttct
     1561 gggctaaatg aaaagcctgt tgtctcgcta ttaagagagt tctcagcccc tgtgaaaatt
     1621 tatgatgatc tggatcagga tgacttgtta tttttattgc gttatgaaac ggatggatat
     1681 gccaagtggg atgcggcaca acgcctggtt cttaactgtc tcaatgaaaa cttgaaattg
     1741 ccagccagtg aatggcaagt ctctaaaact cttgttgctg cctttcagca tgttctgctt
     1801 gatgactctc ttgatatgga tctcagggca gagttgttga cacctccagg atttgaagaa
     1861 gtggcagcta ccatgaaatg ggttgatgtc agcaaggttg aatcagtgcg agattatttc
     1921 cgtcaacaat tgggattggg cttatacaca gaggcgtctg atctttataa agaattgtgg
     1981 gaagtagaag atcatcgcat gcatggccaa gcttatggac gcagaaaatt aagaaatgtt
     2041 tgtttatggt tgatgatgaa agcgaaagaa tcagatgctt tagaattatg ccgagaacaa
     2101 ttcaaggctt ctcgtaccat gacggatcaa attgccagtt gcagcttact agtcaattgc
     2161 tccagggaat ctcaccgaaa tgaagccata gaaggatttt ataaacaatg gtctcataat
     2221 gaattggtac tggataagtg gtttgcgatg caagcggcat gtgagttacc aggcacattg
     2281 gatcatgtca aaaaactaac tcaacatcct gctttttgta ttaaaaatcc aaataaagtt
     2341 cgggcgctgg ttggcgcttt ttgtatggct aaccccagaa acttccatgc gttggatggc
     2401 agcggatatg tctttttaag tgaggtattg attaaactgg acagactaaa tcctcaaatc
     2461 gccgcacgct tggctacgcc ttttacgcgt tggcgaagtt atgatgaacc aagacaaaag
     2521 ttaatacaga accagttgga tcagcttaca aaactggact tgtcgcggga tttgcgcgag
     2581 gttgtagata agagtctgat tgttgaggaa aaataggatg aattaaagtg tgctggattt
     2641 tgctataggc agattcagca cgacttaaac gatttttatc cttttatttg tgcaaacaaa
     2701 gcttacaaaa ttcataaatt aacttttcag cttgattacc ggggcgcgat ttaagaacaa
     2761 cttggattga tgtgcttgtt gcgtgagaac agtttataat taggaaaatc aatgaaacct
     2821 ataggataaa ggagtttttg tgcttcgtcg ttttgcagtg ataggtaatc caattgcaca
     2881 tagtttgtct cctgtcattc atcaaatgtt tgcccaacaa acccaaattg aattgattta
     2941 tgaaaaaatc ctgggtgatg atgtcaagtt tgaacaacaa atctctgatt tttttatcca
     3001 gtatggcaac gggcttaatg tcactttacc ttataaaaag cgcgcttatg aattggcaaa
     3061 aatacgcacg caaagatgcg ctctggctgg agtagcaaac accctatgga tggaggagaa
     3121 tcaattacat gctgataata ccgatggtat tgggctgata cgtgatttat cccgatttct
     3181 tgagttaaag gataaaaaaa ttctgattct gggggctggt ggagctgcaa gagggattat
     3241 ttttccttta ttggaagcga aaccgttaaa attaattgtt gctaatcgga ctttggaaaa
     3301 agcagaagaa ttaaagcgcc aatttcctca aatcaatgtc acaagttttg ctgagttgcc
     3361 agaattcttt gatttaatca tcaatgccac ttctgcgagt ctgtcagatc aggtcattgc
     3421 attacctgaa gaggcgttct cccataaacc attttgttat gatttggctt ataatcaaaa
     3481 aacaagcacc gcctttgttc aatatgctcg taatggaggg tgtgaagcag ttgatggatt
     3541 aggtatgtta gtagagcaag ctgcggaagc attttttata tggaacaagg tgatgccttc
     3601 tacccaaaaa atattagaac acctgcgatc tttttaaact gggggcttgt ataaccgcta
     3661 gatgtgtttt ggcaaccaaa ctctggttaa ctaaaaaaag cgctgacttc tgtggctgca
     3721 gcaatggtgt cttggtagcc taattccagt aattctcgag taaattcttt ttcaaagagt
     3781 aaaaagctaa gcagatcgcc ggaatgactc ctggccccca taatatttaa taaaaatcga
     3841 agcaatactg gcatgctggt gtaatggctt tgggctactg aggccattgc tcggcttgga
     3901 cgtaaatgca gggttgagat aggtctccag ggagagcgac gttttttcca catggataag
     3961 agtgtcgcta tttcattcat acgattgacc atctcaatgt ctctatccag attatccaga
     4021 aaaagcccat tcatcatatt gcctagaata tgggcgaaag caatttcacc attgcgcaag
     4081 gtttctggat cagtaaactc gggcaattcc ctggtaccta agatgagaat tctatccact
     4141 tgaaaacgaa tagcaccgcg aaggggggcg accaagccta tgcttccatc accatagtgg
     4201 aatccattga gactgacagg gggaaagaac aatggtaagg cgcttgatgc tagaatatgc
     4261 tgggcgttga tcacggcacg ttggctgcta tgacggggat aattccaatc ttcaaattct
     4321 gaatgattat gagcgtagaa agagatggtt tgatgagttt cataacaggc gcttaagact
     4381 tcgaaagtat cgaggtatcc ttcttgtata ttttgttcaa ttcgtgtgaa gtcaatgctt
     4441 tcttccagaa attgacgtaa tggtgttgta tcaagcaaat gccctgattg gcgttgtttg
     4501 ataattaagg tgcttaaatt acgcattact gatttgctta attcgtaatt acttgccttg
     4561 tagatttgtt gacaatgaat ttcaccccaa atggcctcaa gtttctccag ggcagcagga
     4621 aaatcattgg cgttttcagc aagtacggcc gcattcacgc tgcctacgct gacaccactc
     4681 actaagtcaa agggtagggt tttggtttgt aaaatatgcc cgatagcttt gagtacacct
     4741 gcctgataag cgcctcgagc accaccacct gccaaatata gagctttttt aaccatcatg
     4801 ttttatttta tttattataa tttgaaatat agttgaaatc tgggctaatt agcaagaaag
     4861 aattatcgat gtcatctttt cttaattatg aattgatata cttaaattaa atcgattctt
     4921 ttcttgagtg attatcatgg gaattacaaa agacgaaaga ttcaaaaaat ttgaaataga
     4981 aaagactctt caactgttac agtcgatgca acgtcctgtt gaatatccat tgactcctac
     5041 agagaaaaca tctttattaa aaaaaaccac aaagcgcatt cctttgctgt caaaattctt
     5101 aaaaactgtt gatgatacgg gtgaagtagt tgtcaaaatt ggtgatagga tgactagtct
     5161 atctcatacg ccaggtaaag aatctttggc tcatggtttt gccttcggtg gtgttgccat
     5221 ggcagggttt gattttcttc ggatccctat tatttatttg tcggcttatt tattaggcga
     5281 agaagtacct gttaatttga gtaataatgc caaatggttt tattctgccc tattgttagg
     5341 gttgaccata actgcattgg cagttccggt aacagcgcct tatatagctt ttgctacagc
     5401 aggcctcagt ttaggtgtgg gtacgttttt attaggaaaa actctttatg aacgctatca
     5461 attaggtcgt gaaagaagaa aattaaaagc ggagatcata agagaagagg atgaaatgct
     5521 gaaaattcag gataaagcga agcatttaga atccctttta caaagcgctg acaatgaaga
     5581 gcaaatagca gccattttat atgaaatatc ggttgtccag gaagactata acaggcagaa
     5641 aaaacacatt cagagtttaa aaaatgacga gttgcaatta gaacaacaaa tcaaaaattt
     5701 aggtacaatg aaggttgtgg acagaactgt cgcagtcgcc ttatccgcgc ttactctcgc
     5761 agccttggct gtgactttat ttttccctcc agttggtgga ggcatgttgg ctgggattgc
     5821 catttttggc ggagcgtatt tcgtggctag agtaagcgcg cctttaatta aagcaacggc
     5881 tgtctggctc tacaataaat taaaatcgac cccaagtgag agtcctgaat cacaggatat
     5941 agataataaa ccgactttaa gtaatgagaa agaagaaaaa aaagaagatt ctttgcaaat
     6001 cgataacgag tttgttttac caccaataga tgctgagacg ttaatttctg aggattcaaa
     6061 ggagtataaa cctgtgcatg aatcaactga ggatgtactg gtggggttat cagtaaatat
     6121 tcaagggctc aaggaaaagg caagagcgga aaaaaaagag gaaatagaag aagcgctccc
     6181 agaggagcct cagcactttt catctctatc cccctcccca agactctcag gccccagaaa
     6241 actatcagca aaagagagta aggatgagga tggagaaagc gagtcagata tagaacatcc
     6301 tgatgtccct aaaatttaaa tctgcattgc aaaaaacaag aatttccttt aagttaaaaa
     6361 aaattactat caaggaatga aaattcatgc tgaatttatt tcgtgagcaa cctcgtgcat
     6421 ttcacatgat ttttatgttg gaattatggg agcgatttgg gttctataca gttcagggaa
     6481 ttcttactct ttattttatt cgctacctgg gttatagcga tttagagtct tactatacat
     6541 ttggtgctta ttctgcgctg gtatatggaa tggtggttat tggcggctat ttgggagaca
     6601 gaattctagg taccaagcga accatagttc tcggtcttgt tgtgctggcc atgggttact
     6661 tttctcttgc agttactgat aagcaacatg tatttcttgc actaggattg atttgtgtag
     6721 gtaatgggct attcaaagcg aatccatcga atttgctatc caaatgttat gatgacaatg
     6781 atccacgact acatggcggc tttacccttt attatatggc cattaatctg ggcagtatgg
     6841 ttgcattatt cgtcggacca gcagtatcaa gcgcttatgg ttactcttat gcttacatga
     6901 ttagtgcaat aggtttgatt ttgggtattg tgaattattg gtatcaacga caacatgtgg
     6961 ctcatgtcaa tacaaaagcg gatcttcgta aaataacagc ctttcactgg ggaacaataa
     7021 tagctggtgt tttaatactt actggaatat cagcttattt attacaacat gtcatgcttg
     7081 ccaaaaatct gttgtggggt attactctct tggtagtgtc tgtttatatc tatttaatgc
     7141 gcaaacaggc tcaggcttct tttatgagaa tgttagtcgc tttggtttta atgattgaag
     7201 cagtcatatt ttttacttta taccaacaga tgcctacttc attaaatttg ttcgctgtga
     7261 ataatgtaat acctactttc ctgggcatca ctattgatcc gcaaagcttt caagctctca
     7321 atccaatatg gattgtatta atgagtcccg tattagctta tttctataga aaattgcatc
     7381 aacataatat tttattttct ataccttata aatttgcatt cggcatgacc atgtgtgcag
     7441 tgagcttttt aatgctgtat tttgctcgtt atttttatgt tggaaatggc atggtttctt
     7501 cctggtggat gattttcagt tatttattcc aaagtcttgg cgagttatta gtatcagctc
     7561 ttggtgttgc aatggttgct gaattggtgc cggctgctaa tgctgggttt gttatgggga
     7621 tgtggttttt aacttccgca gtggcagggt ttattggtgc tactgtcgcg tcttttacag
     7681 cacttccaga gaacatcaag ccaggggttg agtctttatt gatttattcc gatgtatttg
     7741 cctgtattgg tcttgttact ctattgattg cagttattat gtggttagta tcacctcggc
     7801 taagccgcta tattacagtc ccttccttgg aaagggataa ggatatagaa acagtagaat
     7861 actctacgac ctatatcccc agtcactagg tttgtttatt tcccttttcg gcaattgagt
     7921 tggttttaat atcaccaatt gccgtttttt atttttgtcc tatattgctt ttgcattctt
     7981 gaatagaaga taaccagaaa ttatttatta ctatttttgt cctattacat atctcttaat
     8041 gttaatttaa taaaaaatgt ataaaatatg tacttgaaat caatctttcc ctaatcatta
     8101 ccgggttaag gttctcaggt tctgtgagtt aatttgcttg gagtataaag tggggcttga
     8161 aaaaaaaatt aagctaagcc agcgttctct ggaattatta aacgattact taaatttggg
     8221 tgggtctggg ccttccaatt tagtgaatga gagttcgtcg attgattttt catccttaac
     8281 tcaactactt gccagtaatc tgtccaaatc atctaaaact tcaaaaacaa taaaaaagaa
     8341 aagagaattt atagaggcag ctttgtatgc cgagttgtta cgtgacttaa gtacttcatt
     8401 gaatgtatcg gatgaggaaa tcaagcctgc agaaaaagaa cctcctgtct ctaataagtt
     8461 gaaattctgg cttttggcaa tagctgggac attattagcc gcatgcgagg ggtttgacag
     8521 cattacaacc atgatggctg tattatcttt tcctgcctgg attgtccttt gtgcaggtat
     8581 tttattttcc gctttatctg tcgttgtatt ttatgggttt gatttagtcc aggtttctca
     8641 aaacctggaa ataaaattaa ctgatactcc caagctattg gatttatacc tgtcacaaat
     8701 ggaagaaatt aaggcgatta gaaaaaaaat tgacaattat tctcttgctt ctatgtcaat
     8761 ggatgagttg aatgagttgc atttaatcct tgttatgctg caagaccgtg tcaactcttt
     8821 agtaaaagca ggcaaaaaat ttgatttggc actgaatagc cccaaaatgg atgttgctaa
     8881 aacaattgtt tctggagtat ctggaatact cttttttggt ggaggttttt ttgccggaca
     8941 atcagtagcg acattcatgc tggctctggt tatgacttct gttacaccta cattttggcc
     9001 agttttatta tttagtgtga ttgtcggctt ggctgccttt agtttgtact ggtatgtgca
     9061 acgcgtagga gtaaataaac tggttagcga atggtttggt ttggatgagg ataaaataga
     9121 aaaattatgt ggaaagtcca agatggacaa agaattgaaa aagctaaaca accttgatga
     9181 aaaggtaatg actacttcgg atttgctctc gaaagtggct aatttggaga gtaaactaat
     9241 agatatcggg aaacaaatga aggagatgag tgcgcaccag gctcctgaaa agaaattaga
     9301 gggtcctgct ggattaaagg caagttctaa tgtgtattcc tttcacttta attcctcaaa
     9361 tcgggatcag gagagcgttt acggtgagag ggtccttgat gatgctgttt ctgattatgg
     9421 taggggtcag aatcataatg attcaaacca tagcctatgt tagccaaatg caaaggcata
     9481 tagattaaca gggagtttcc ttgcaaataa tccggattgg ctgacatata aaactctttt
     9541 aattcactgc tctcttcatt aattcttgag agctgacttc ttaaaggaac cagtggggaa
     9601 ttgacaacca taggatgatt attggttgaa atccagccgt tttgtttttt aagggcgttt
     9661 agaacggtca atatttgatc tggaaccctt tgtaatggtt taggatatat taggtcactc
     9721 aattgataaa taaattcgta agttaacggg ttgttggcaa atgctatgta aaccaattcc
     9781 tcgtgaggat tggctaactt gtattttatc gcctgagtta ggccggcact tttaatagat
     9841 gcgtgaattt ggtattcagg atttagatac aaataagcga tataacaagt cacttgtttt
     9901 tttcccagat caacagattg tctgcagtta cggctgaatc cggctatttc tttattttct
     9961 ccatagagaa ccgttaactc accatacaaa tcaggctgta tgatgaaacg tataaattct
    10021 tcttggctgg ccaggtgata atacttggag aggaagcgat ataatttatt tattaatttt
    10081 aaggagattt ttttcttttc ttcatctaca acataagagt ggatcgcttt aacatcgtgt
    10141 ctgttcattg ctctctccat gaaacatttt tttcgcttt
//
LOCUS       DATNSN010000122        10151 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.122, whole genome shotgun sequence.
ACCESSION   DATNSN010000122 DATNSN010000000
VERSION     DATNSN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.122"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(89..904)
                     /locus_tag="VAH76_002346"
                     /pseudo
     CDS             complement(89..904)
                     /locus_tag="VAH76_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611091.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1141..2349
                     /locus_tag="VAH76_002347"
     CDS             1141..2349
                     /locus_tag="VAH76_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1386948.1"
                     /translation="MSDISRGDIRVKGFTDSEMDFQLIRQLGSSSYKAASVGECLNIA
                     NTMKNEDPEEWVRQFERLAEWQKKDGLERMVKNHVISGREQLFKASNSFRAAEYYSPP
                     SSEHHRKLGLNSADCFCTAISSMDIHFENHSIPYKNINLPCYFISPANDGEKRDTVMI
                     VSGFDGTLEEEFLMRGLAAVERGYNVIHFAGPGQMDVFRKYPDTYFEPDFEQVVKRII
                     NYFEFRQEINMAKLTLMGISFGGYFATRAASHEPRIKVLIANSPILDLHAYMCSFVKM
                     DPCEMPDSEDFSINDLPSIPNEVMPPQLKAQTEHLIRRFGQPTFKATFNYLREFTVGN
                     SLNHIKIPCLALLGSGEGGEPGKQFAEFCKTTNSAQYEFSDFEGAGSHCQVGNVSYSN
                     AVVYDWLDSL"
     gene            2436..3704
                     /locus_tag="VAH76_002348"
     CDS             2436..3704
                     /locus_tag="VAH76_002348"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEO1386949.1"
                     /translation="MLPDDSKKKSKGHIVLSSHPASHTQAPLKIKWGEKNPKERGPVI
                     ASLTNIKNRNAVGTHSGSYSVYRALAVAAGLLDPEHVPDLTDTLPPVNIGPHRQWGEA
                     GKIVSLDPWGHLVASVFSEEIKAGYDIRPTIAVTRAHINIPELHIAMQKGRLKPDGKI
                     LSETGDVVVTKAAIEPVWYLPGIAERFQVSEALLRRTLFEQTAGMFPELVTRSDLDVF
                     LPPIGGLTAYFFGDVTTIHNPEIELTCRIHDECNGSDVFGSDICTCRPYLVHGIELSI
                     ESAQKGGAGLIVYNRKEGRALGEVTKFLVYNARKRQKGGDTAAKYFERTECVAGVQDM
                     RFQELMSDILHWLGITKIHRFVSMSNMKYDALLKSGINVIERVKIPDELIPPDARVEI
                     DAKRAAGYYSPEGIIDINELAKPKGRALHE"
     gene            3697..4938
                     /locus_tag="VAH76_002349"
     CDS             3697..4938
                     /locus_tag="VAH76_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HEO1386950.1"
                     /translation="MNNQEQQIQNVLNTLRDPRTIRTRSHRILNLAKQNQLEHFSLSP
                     ERMTTTASYIVDIITSRYPELDIPYHSRWRHFEMDGLPRIHNLRKQLEPISPSEWGKI
                     LYELVIISVFLDAGAGPLWRYKEALTEKEYSRSEGLALASLYLYESGAFSADPTNPFR
                     VDAERLLDFKETDLIKGFQVSSTNLLEGIPGRVSLLNRLGEIIHRKEHCFNRRRRLGE
                     FYTYVESLQDNKILPTTKLFEAVLEVFNGIWPVRLIYHGVSLGDVWIHSALKTEEPGS
                     EYIPFHKLSQWLTYSLVEPLEQTGINVTDLDVLTGLPEYRNGGLLIDTELLKVRNNKI
                     LEQPQDPGSEPIVEWRALTVALLDELALLIRQQLNMNAESLPLAKILQGGTWEAGRQI
                     AQKKRINGTPPIEIISDGTIF"
     gene            4951..5595
                     /gene="upp"
                     /locus_tag="VAH76_002350"
     CDS             4951..5595
                     /gene="upp"
                     /locus_tag="VAH76_002350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO1386951.1"
                     /translation="MDFNQVKVINHPLIQHKLTIMRKKETSTVKFRTLMHEVSMLLAY
                     EVTRDLEIEYEEIETPLATMQSPVLKGKKLVFVSILRAGNGLLDGMLQLVPTARIGHI
                     GLYRDPKTLEAVEYYFKLPEHTQDRDVIVVDPMLATGNSAIAAVKEVKALHPKSIKFL
                     CLLASPEGISNFHGEHPDVPIFTAAIDEQLNDHGYIVPGLGDAGDRLYGTKLAH"
     gene            5901..8801
                     /locus_tag="VAH76_002351"
     CDS             5901..8801
                     /locus_tag="VAH76_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386952.1"
                     /translation="MLELNDKETAVIKAIHHNHKKRLESQMITRFGVYFEQSNHPEKL
                     YSWIEKIEDTRLLANHYLDTIALSRAPSTISSLEKETAGSSYYIEKAKLLIPESIRNF
                     LSDINSSNDNTLFNFVAAEVFSLALGKSITDLLFDYFNNEFLGAPENVQKEFNETLEK
                     QTELSNLIKKECERISIEIIKLFHFREMLLLGKISKSPIEPNSREEFITKAKIANQSG
                     ESVHLAIEIYFARELSRIFNSGFKSLYQVHEVEIQSEHQSPLVKWINQMSESEQVRLA
                     FTQEIQLKFMLLARDFLLEKMNQKGILARYPIIFSLFAGVIIAPIVGVTMIIMTGGTF
                     LWAIAALAVLVFALSCIATHLLINKLSVLTYQRSSDNRAHIQQSIDMINGEFLRLKKE
                     SLLKKETTSEVIENTRDFQKINQNFHLVTIGKKVARGSVSGWLREYASRYRHSKAIEI
                     DLGDEYKELITQSRIQTQELIHSINKKNSKLLIKWIHDTMTYLQDEKNQSVIEEFELI
                     PKMKEQVLEIVCQLNYIPPMLLKFYSNSREKGGLGGNESDFSHIKRLTPIDAPDKPKH
                     NPYNYLCDTALNLFKKYEPLYTSTCIFLGDPEYRQMLGIARRDAGEPLTAENINRYLD
                     NSYAFLLSLCHKIKPGLGLDPLQQSARVTDEFVLYRMLLLKQLAAISESDSKEISNEI
                     KFKIRLFIQKYFNQDTKIIFDDLENQMFLLNKEAPNSRIYKNSDHFEVIDTELDNIFQ
                     AITLDMAYNSNNFRFIDILGYFINKFIKEHDSQSATVFAYGKAEQEINPQGTKYYLKT
                     LAQYCNNTTEFLKSAQTNTTLTATNLLDCYRYNISLQVYRTQIRIICSIKEINKLKMT
                     NETSEQITLLLKSFQQLAEFAKIHCFLLKTDSSCYRLFKLIENKAMQSTLSRDWIRQL
                     DAKEALVYMMDDLPNQLKLASDYNTSSLFFSSAKRAKREKMETPEEPIMKPA"
     gene            complement(8961..>10151)
                     /locus_tag="VAH76_002352"
     CDS             complement(8961..>10151)
                     /locus_tag="VAH76_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEO1386953.1"
                     /translation="IADVFIKIIKMLISPIIFLTLVSGIAAMNDMRQVGRLAGGALLF
                     FTFITTLALLLGLLATDYFKPGVGLNVNPNSLDVKEASSYLEGAHKVTNVTDLLINII
                     PKTFISAFVDGELLQVIFISILFAIGLILTGNAGKSLVTGMQTLSNVFFKIIHFVMYM
                     APVAAFAAMAFSVGKFGVGPLFNLIGLLACYYLTSIFFILIILGSLLQWYCKINIWHL
                     LRYLKDELLIVWSTASSETVLPSLMQKLERLGCEQSVVGLILPLGYSFNLAGTAIYLT
                     MAAMFIAQATNTDLTVWQELSLLGVMIISSKGAAGVSGSGFIVLASSLATIGHIPAAG
                     VVLVLGIDKFMNEARSIINMMANAIATIIISRWQQSLDYQKTQFELQEKGPAETKNVY
                     ASGM"
BASE COUNT         3271 a         2076 c         1927 g         2877 t
ORIGIN      
        1 attcagccac cacaaacaag agaacgaatt ttatagaagg ataacggcag accttattgt
       61 tcttaattag atctcttgta taacctcttt aaagctcaaa attataaatt ccagcaatta
      121 aattaaatct caaatcaaac cgttttcttc tgtttctata gcgaacagca ataattttga
      181 agcgcttaag catggcaatc acattctcgt tcaaaacgcg tttgctggac aattcacaat
      241 ttttattttt atcctccttg gccagatggt tcttttttat tttttggcaa ggtcgagttt
      301 tgatgtaatt tctgtatttc ttggtagctt gtgtctgtta agcttctagt ctctggatga
      361 atacgagcat tagactcttt aaataaccta aagtcatgtc atttgctatt ggtaaaaatt
      421 ggtgcaaata atctcttttt tcttcttatt cacgataatt tgtgtcttta tcgtgtgtgt
      481 tttctttctg tctgaataaa agtatttttg ctttttccag gtcgctcaat gggtgtctca
      541 gttacatcaa ttaacacaac gtcatattca acttcacttt ttagtagcat tttacaccca
      601 ggcagtgaaa acgtcccatt ttgatgagca tatcttcaac ccatcgtata ttccgatagc
      661 aggcgctttc actaagcccc taactacgtg aaatatggaa ataggttcta tattcacgca
      721 aatactccag agtcattagc aaccgttctt ccactgctaa tttattgggt ttgccacctt
      781 ttacttttaa ttcttcttcg gcctttctca atatctctgt catcacatta aatgtggtgc
      841 gttttatccc tgttaatctg cgaaatcctt cctctgactc ctctctaagt tgttcgaatt
      901 tcatctaccc cctgaaaaaa gaaaaaaatt taatcaattc gaaaggttat gcaagaactc
      961 tattaaaggc tactcctgtt taaagataat ggataagttt attctcatag ccgttctccg
     1021 aattttcaga acgaatggat aaatcattaa cgctgtttaa ttaaggttga tgttattaga
     1081 atgtgtcgtt actatttaaa ttaatatact ctgtaaaaac ggaaataacg gagaagtaaa
     1141 atgagtgata tcagtcgtgg cgatattcgg gtgaagggtt ttactgattc agaaatggat
     1201 tttcaattaa tacgacaact gggatctagc agctataaag cagcatcagt cggcgaatgt
     1261 ttaaatattg caaacaccat gaaaaatgaa gaccctgaag aatgggttag gcagtttgaa
     1321 cggttagccg agtggcaaaa aaaggacggg ttggaacgca tggtaaaaaa ccacgtcata
     1381 agcggtcgag agcaattgtt caaagcctcc aattcatttc gtgccgctga atattactct
     1441 cctccttcct cagaacatca cagaaaatta ggattgaatt cagcagattg cttttgcaca
     1501 gccatctcga gcatggatat ccattttgaa aaccattcca ttccttataa aaacatcaat
     1561 ctcccatgct atttcatttc gcccgccaat gatggagaaa aacgagatac cgtcatgata
     1621 gtcagtggct ttgatggaac tttggaagaa gagttcctca tgcgtggatt ggctgcggta
     1681 gaacgtgggt ataatgtgat tcatttcgca ggccctggcc aaatggatgt ctttcgaaaa
     1741 tatccggaca cttattttga gcctgatttt gagcaagtgg ttaaaagaat cattaattat
     1801 tttgagttca ggcaagagat caatatggcc aaattgacat tgatgggcat tagttttggc
     1861 ggctatttcg caactcgcgc cgcgagccat gaaccaagaa tcaaggtttt aattgccaac
     1921 tcccctattt tggatttaca tgcctatatg tgttcctttg taaaaatgga cccttgtgaa
     1981 atgcctgatt ctgaagattt tagcatcaat gacttaccct ctatacccaa tgaagtgatg
     2041 ccccctcaac tcaaagccca aacagaacat ttaatcagaa gatttgggca acctactttt
     2101 aaggcaacat ttaattattt aagagagttc accgtaggta attcattaaa tcatattaaa
     2161 atcccttgcc ttgcattatt aggtagtggc gaaggaggag agccaggaaa acaattcgcg
     2221 gagttttgca aaacaacgaa ttctgcacaa tatgaattta gtgattttga aggggctgga
     2281 agtcattgtc aggtaggcaa tgtttcttat tccaatgctg tggtttatga ttggttagac
     2341 agtttataac aacagtgtgc acctgcttat aaagggataa aagataaaac aaccgattta
     2401 tttgagcacc gttgaaacaa aaggaattaa gtatcatgtt accagatgat tcaaagaaaa
     2461 aatcaaaagg acacattgtc ttgtcttcac acccggcaag ccatacacaa gcaccattaa
     2521 aaatcaaatg gggtgaaaaa aaccctaaag agaggggacc tgttattgcc tctctcacca
     2581 atatcaaaaa tcgaaatgca gttggaactc actcagggtc ttactctgtt tacagggcct
     2641 tggcagtggc agctggtctg ctggatcccg aacacgttcc tgatctaacc gacaccctgc
     2701 cccctgttaa catagggcct cataggcaat ggggtgaagc aggtaaaatt gtctccctcg
     2761 atccctgggg gcatcttgta gcttctgttt tctcagagga aataaaagcc gggtatgaca
     2821 tcagaccaac cattgccgtc acaagggcac atattaatat cccggaattg catatcgcca
     2881 tgcaaaaagg cagattaaaa cctgatggaa aaattttaag tgaaacaggg gatgtcgtgg
     2941 tgactaaggc cgcgattgaa ccagtatggt acttgcctgg aattgcggaa cgctttcagg
     3001 tttcagaagc attgctcaga cgcactcttt ttgaacaaac agctggaatg ttcccagaac
     3061 tggtgacccg gagcgacttg gatgtctttt tgccacccat tgggggatta actgcttatt
     3121 tctttggtga tgtgactacc atccataatc cggaaatcga attaacttgc cgtatccatg
     3181 atgaatgtaa tggctccgat gtctttggtt ctgacatatg cacctgcagg ccatatttgg
     3241 tccatggaat cgaattatcc atcgaatccg cacaaaaagg aggcgcaggc cttatcgttt
     3301 ataatcgcaa agaaggaaga gctcttgggg aggtcactaa atttctggtt tataacgcca
     3361 gaaaaagaca gaaaggtggt gacactgccg ccaagtactt tgaaagaacg gaatgcgttg
     3421 ccggtgttca ggatatgcgt ttccaggaat taatgtccga tattcttcat tggctgggga
     3481 tcactaaaat tcatcgcttc gtttcaatgt ccaatatgaa atacgatgcc ttgctcaaat
     3541 ctggaatcaa tgttattgaa cgggtaaaaa taccggatga gttgattcct ccagatgccc
     3601 gggtagaaat agatgccaag cgtgcagcag gatattactc acccgaagga attattgata
     3661 tcaacgaatt ggctaaaccg aaaggacgtg cccttcatga ataatcaaga gcagcaaata
     3721 caaaatgttt tgaatacact tcgtgatcct cgcaccatac gaacccggtc gcatcgtatt
     3781 ttaaacctgg caaaacagaa tcagctggaa cacttttccc tgagccctga acgaatgaca
     3841 acaacggcat cgtatatcgt agacattatc accagccgtt atcctgagct tgatattcca
     3901 tatcacagcc gctggcgtca ttttgaaatg gatggccttc cccgaattca taatttacgc
     3961 aaacaactgg agccaatttc cccctcagaa tggggaaaaa tactctacga actggtcatc
     4021 atcagtgttt tccttgatgc aggggcaggc cccctatggc gctataaaga agctctcact
     4081 gaaaaggaat acagtcgatc tgaaggatta gccctggcca gtttatacct ctacgaaagt
     4141 ggggcattta gcgctgaccc aactaacccg tttcgcgtgg atgccgagcg tctgcttgat
     4201 tttaaagaaa cagatctcat aaaaggtttt caggttagct ctaccaatct gcttgaagga
     4261 atacccggtc gagtatcctt gcttaatcgc cttggagaaa tcattcatcg taaggaacac
     4321 tgttttaatc gccgaagacg tcttggtgag ttttatactt atgtggaatc cttgcaagac
     4381 aacaaaatac tgcctactac caaattattc gaggccgtgt tagaagtatt caacggcata
     4441 tggcctgtac gactgatcta tcatggagta tctttaggtg atgtgtggat ccacagcgca
     4501 ttgaaaaccg aggagcccgg atctgaatac ataccctttc acaaattatc gcaatggctc
     4561 acttattctt tggtagaacc actggaacaa acaggtatta atgttactga tttggatgta
     4621 ttgaccggat tgcctgagta tcgaaatggt ggtttactga tcgacactga actcttaaaa
     4681 gtaagaaata ataaaattct ggagcaacct caagaccctg gctcggagcc catagtagaa
     4741 tggcgtgctt taaccgtagc cttgctggat gagttagcgc tattaatcag gcaacagctc
     4801 aacatgaatg cagaatcgct tcctttagcc aaaatactgc aaggaggcac ttgggaagcg
     4861 gggcggcaga ttgcccaaaa aaaacgaatt aatggaacac ccccaataga aattatcagt
     4921 gatggcacga tattttaata aggaataata atggacttca atcaagttaa ggttataaat
     4981 catcctctca ttcagcacaa actcaccata atgaggaaaa aagaaaccag cacagtaaaa
     5041 tttcggacac tcatgcacga agtcagtatg ctattagcct atgaagtcac tcgagatttg
     5101 gaaatagaat acgaagaaat agaaactcct ttggccacca tgcaatcacc tgttttgaaa
     5161 ggaaaaaaac ttgtttttgt ctcgatatta cgcgcgggta atggtctact ggacggcatg
     5221 ctacaattag ttcctactgc ccgaattggt catataggtc tctatcgaga tcccaaaacg
     5281 ctggaagcag tcgaatacta cttcaagcta cctgaacaca cacaagacag agacgttatt
     5341 gttgttgacc ccatgctggc gacaggaaat tcagccattg ccgcggttaa agaggtcaag
     5401 gcattacatc ccaagtcaat taaatttctt tgtctactgg cctcgcctga aggaatttcc
     5461 aattttcatg gagaacatcc tgatgtccca atattcaccg cagccataga tgagcagctg
     5521 aatgatcatg gatacattgt tccaggttta ggagacgcag gagacaggct atatggcaca
     5581 aaactggctc attaatgctg aattatcctt atttcaggga tacccaatca gccaatagta
     5641 cgtttaaata ataattaaga ttgtcttgcc ctttcagtaa tgactgcttt tactttttga
     5701 caagggcatg ctgaaaaatt accaccaatt tttctgtgga ctccctctga gtgtttatgc
     5761 gggacaacag atgtgatgta tcaagattct gaagaatttt ggcagggaaa tgccgggaaa
     5821 caagcaacag caaatatagt ctcttggcat accccttatt gcataaagtc atatctaatc
     5881 actctacgag ggagcgaaga atgttagaac tgaacgacaa agaaactgca gtcattaagg
     5941 ctattcatca taaccataag aagcgacttg aaagccaaat gataacccgt tttggggtat
     6001 attttgagca atcaaaccat cctgaaaaat tatattcctg gatagaaaaa attgaagaca
     6061 cgcgcctgtt agctaatcac tatctggata ctatcgctct aagccgagct ccaagcacaa
     6121 tatcttcatt ggaaaaggaa acagcaggtt cttcctacta cattgaaaaa gcaaaactgc
     6181 ttattccgga atctataaga aattttcttt ctgatatcaa ttcttccaat gacaatacct
     6241 tattcaattt tgtagccgcc gaggtctttt ctctcgcttt aggcaaatca attaccgatc
     6301 tgttattcga ttacttcaat aatgagtttt taggggcgcc agagaatgta caaaaagaat
     6361 ttaatgaaac cctggaaaaa caaactgaat tatccaattt gataaaaaaa gaatgtgagc
     6421 gcatctccat agaaattatc aaattatttc atttcagaga aatgttgctg cttgggaaaa
     6481 taagcaaatc accaatcgaa cccaattcca gagaggagtt cattaccaaa gcaaagattg
     6541 ctaaccaatc tggcgaatct gttcatttgg ctatcgaaat ctattttgca cgtgaattaa
     6601 gtcgtatttt taactcaggt tttaaatcac tttatcaagt ccatgaggtg gaaattcaaa
     6661 gtgaacatca atcgcctctg gtcaagtgga tcaatcaaat gtcagagtct gaacaggttc
     6721 gattggcctt tactcaggaa atccagttga aattcatgct tctggcaaga gattttttac
     6781 tagaaaaaat gaatcagaaa ggaatactcg caagataccc tatcattttt tcactctttg
     6841 caggagtcat tattgctcca atagtcggtg tgactatgat tattatgact ggcggtactt
     6901 ttctatgggc gatagctgct ctggctgtcc tggtttttgc tctctcttgc atagcaacgc
     6961 acctcctgat caataaactc agcgtattaa cttaccaacg ttcttcggat aatagagctc
     7021 atattcagca atctatcgac atgataaatg gagagttttt acgtttgaaa aaagaaagtt
     7081 tattaaaaaa agaaacaacc agtgaggtta ttgaaaatac aagagatttt caaaaaatca
     7141 accagaattt tcatttagtc acgattggta aaaaggtagc ccgaggctca gtatccggat
     7201 ggttaagaga atacgcttct cggtataggc acagtaaagc catcgaaatc gatttgggag
     7261 atgaatacaa agaactaatt actcaaagca ggatacaaac tcaagaactc attcattcga
     7321 ttaacaagaa aaactccaag ttattgatta aatggattca cgataccatg acttatttgc
     7381 aggatgaaaa aaatcagtct gtgattgaag aatttgaatt aatccccaaa atgaaagagc
     7441 aagtccttga gattgtctgt caactcaatt acatccctcc tatgttattg aaattctatt
     7501 ctaactccag agagaaaggt ggcctgggag gtaatgaatc cgatttttct catataaaac
     7561 gtttgacccc catcgatgcg ccagataaac ccaagcataa cccctataat tatctctgcg
     7621 atacagctct gaatctgttt aaaaaatatg agcctttata tacttcgact tgtatttttt
     7681 taggagaccc ggagtatcga caaatgttag gtatcgccag aagagatgcc ggagagcctc
     7741 taacagcgga aaacatcaat cgttatcttg ataactccta tgccttttta ttatcgcttt
     7801 gccataaaat caaacctgga ttaggcttgg atcccttaca acaatcagcg cgcgtcactg
     7861 atgaattcgt tctctacaga atgctgttgt taaaacagct ggcagcaatt tccgaatctg
     7921 atagcaaaga aataagcaat gaaatcaaat ttaagattag actttttatt caaaaatact
     7981 ttaatcagga taccaaaatc atttttgatg atcttgaaaa tcaaatgttt ttgctaaaca
     8041 aggaagcccc caactcccgt atctataaaa acagcgatca ttttgaggta attgatacgg
     8101 aattggataa catttttcaa gccatcactc ttgatatggc ttataactcc aataatttca
     8161 gattcattga tattcttggc tacttcatta ataaatttat taaagaacac gactcccagt
     8221 ccgcaacagt atttgcttat ggaaaagcag aacaggaaat caaccctcaa ggcacaaaat
     8281 actatctcaa aactcttgct caatactgta acaatacgac cgaattttta aaaagcgctc
     8341 aaacaaatac aacattaaca gccacaaatc tccttgattg ctatcgatac aacatcagtt
     8401 tgcaagttta tagaacgcaa attcggatta tctgcagcat aaaggaaatc aataaattaa
     8461 aaatgacgaa tgagactagt gaacaaataa cccttctact caaatccttt caacaacttg
     8521 cagagttcgc aaaaatccat tgtttcttgt taaagactga ctcttcttgt tatcgattat
     8581 ttaaactgat tgaaaacaaa gcaatgcaat ctactttatc cagagattgg ataagacagc
     8641 ttgacgcaaa agaagcgctc gtttacatga tggatgatct gcctaatcaa ttaaaacttg
     8701 cttcagatta caacacttcg agtttatttt ttagttcggc aaaacgagct aaaagagaaa
     8761 aaatggaaac cccagaagaa cctataatga agccggccta aattaatttg aagggattat
     8821 tggtaagtaa aaacataata gactggagtc ttgatgtcca acaaaaggac atcaagactt
     8881 ttactgcaag cctgtttttt tgacaggtta aatactcctt tcacaacaga acagggctta
     8941 tgaaaagccc cgtagaaatc ttacatccca ctagcataaa catttttggt ttctgctggt
     9001 cctttttctt gcaattcaaa ttgagttttc tggtaatcca aagattgttg ccacctggaa
     9061 ataataatcg tggcgattgc attagccatc atattaataa ttgacctggc ttcattcatg
     9121 aatttatcga tacccaaaac aagaacgacg cctgcagcag gaatgtgtcc tatcgttgct
     9181 aatgaactcg ccagcacaat aaatccgctt ccggacaccc cagcggcacc cttggaacta
     9241 atgatcataa ctcccagaag cgataactcc tgccaaactg ttagatcagt attagtagcc
     9301 tgtgcaataa acatggcagc cattgttaaa tagattgcag ttcctgccaa attaaaagaa
     9361 tatcctaatg gcaggatcaa accgactaca gattgctcac accccagtcg ctccagtttc
     9421 tgcatcaaac ttggcagtac ggtttcggag gaagcggtgc tccacacgat taaaagctca
     9481 tcctttaaat accttaacaa atgccaaata ttaattttgc agtaccattg caaaagactt
     9541 cccaagataa tcaagataaa gaaaatggaa gttagataat aacaagccaa taatccaatt
     9601 aaattaaaca aagggccaac tccaaatttc ccgacactga atgccatggc agcaaaggca
     9661 gctacaggcg ccatatacat tacaaaatga atgattttaa aaaaaacatt agacagggtt
     9721 tgcattccag taaccaatga ttttcctgca ttgcctgtaa gaatcagacc gatcgcaaac
     9781 aatattgaaa taaaaataac ttgcagcagt tcaccatcga caaaggcgct aataaacgtt
     9841 ttggggataa tattaattag cagatcagtc acattagtta ccttatgcgc accctcaagg
     9901 taagaactcg cttctttgac atccagagaa ttgggattca cattcaaacc aacaccaggc
     9961 ttaaaataat ctgtcgccag aagacctagc agcaaagcca gggttgtgat gaacgtaaaa
    10021 aaaagtaatg ccccacccgc caagcgtcca acttgcctca tatcattcat tgctgcaatt
    10081 ccagagacca gagtgagaaa tataatagga gaaattagca tcttaatgat tttgataaaa
    10141 acatcagcta t
//
LOCUS       DATNSN010000123        10105 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.123, whole genome shotgun sequence.
ACCESSION   DATNSN010000123 DATNSN010000000
VERSION     DATNSN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.123"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..326
                     /locus_tag="VAH76_002353"
     CDS             <1..326
                     /locus_tag="VAH76_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1386954.1"
                     /translation="SYGDTFINRYTPVFSFTKVSDQEFTIELAKIGEAFYTIPSELSS
                     SQEKAAHLITLLTRAEESQVTDMHKILNSFVSGKITSGSMFNFENDGSFKRDPEEAYN
                     LINKI"
     gene            553..972
                     /locus_tag="VAH76_002354"
     CDS             553..972
                     /locus_tag="VAH76_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEO1386955.1"
                     /translation="MKVLYTTIAKTHGGRNGHVETTDGLLKLDLAMPRELGGEGGATN
                     PEQLFAAGYAACFESAIRHVANMQKISLEDVSMTSEVSLYATPEKGFKLGVALHAYIT
                     GLNQNKAEALVAKAHEVCPYSNAIRGNVEVKLSVSVK"
     gene            1007..1630
                     /locus_tag="VAH76_002355"
     CDS             1007..1630
                     /locus_tag="VAH76_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEO1386956.1"
                     /translation="MYTLYSFGTPNGFKPTIMLEELAQPYTIKLIDITQGEQFNPDFL
                     KISPNNKIPVLYDQTNDFYLMESVAILQYLAEKHRQFLPETTKEKYAIIQWCYFQAAH
                     IGPMLGQFGHFHRYAQEQVPYAMKRYADESMRLLGVMEKQLAKTSFIGGANYTIADMA
                     IWPWIWCFQFIYEQTIDAKLFPSLMSWYQRVSERPAVRATLAAYGRL"
     gene            complement(2233..3615)
                     /locus_tag="VAH76_002356"
     CDS             complement(2233..3615)
                     /locus_tag="VAH76_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEO1386957.1"
                     /translation="MKLIPSQLRSAIPGIIWPPAPHPMAAQLYYQLDNFNHTEKLPPS
                     EIIKNQFKQLVALMSFSRQCVPYYQKKFAYLPSFEKWQDLHKAWQELPLLKRETLQIA
                     KDNIFALQVPSGHEPSELLSTSGSTGQPVTVKGNMATQFFWNAISLRNHLWHNDEFHK
                     SFASIRYTENKEALPPLGAKFLNWSPATYSIVETGPCYQLTLCTPEQEAQWLQRINPD
                     YLNCNPSTLREITLYFAKHGNKPENLQKVHTHSEIVEPELRSLVKEVLGIPLIDNYSS
                     KECGYIALECPVSGYYHVQSENVLVEILDESNNPCKPDQPGRVVITTLHNFSSPLIRY
                     DIGDYAIPGESCSCGRNLPVLKYILGRKRNMIQMPDGRKLWPAFTSNGLRLMDLFSGS
                     QFQLVQTTPTEIQVNLKHPDSLAYQEEQKIREKLSIIFDYPFHFVFNYVDSIPRSAGG
                     KFEDFISLIS"
     gene            complement(3660..4637)
                     /locus_tag="VAH76_002357"
     CDS             complement(3660..4637)
                     /locus_tag="VAH76_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386958.1"
                     /translation="MTALRKTTLSLFIIGFCNTHSSIAGTMGPVKTGCSGNRLFAGAE
                     ALYLQPRNDDLDYTTYLVDNYTKTLNTHLDYDWGFRLYGGVKFANNNDITIAWQRLHT
                     RDNDMLLGNGDIAIEPRWLNLAAWASVQSENIFDYDEVSGVFAHTHDFNNAWLIRYGA
                     GAEYAEIDSRLTVNGIEQTIQGHTNISNFQGFGPRIEADVFYNFYREFNVFASANSAL
                     LIGSRDVSLLAHHPFGVSRDFSDRNTIVPKLGLRIGLDYQRPFGLIGDGAASTIDFQV
                     GWQAETYIDAIERPSTGLVGGEGVVVLPQRSNYSNQGLFFGMKLTSGCL"
     gene            5158..6951
                     /locus_tag="VAH76_002358"
     CDS             5158..6951
                     /locus_tag="VAH76_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1386959.1"
                     /translation="MALSEIIINDVVQYQANAVISESNYHILRQYLLERKPLSRVLNP
                     INSLLSEHCDKDKQAIINHLTQLACESQRNYDIQEAMSDEQERVNENSLMINYRSELL
                     TLENTLKQLDTKCFQQQRQYNQVSRQFNELKVNLTHINQSIDRIRYERQLLDQQLNHP
                     YSDGNEYTNPPTHTRIYPDLSLPRAPLTRDRLLQEENRLIEERQRLMHLVNSKEAEKE
                     KEEQNLNRLHKEKKETEGRYSEVKHQIDIALPNSEQQRQIRNQERLAREQARASYDPH
                     LLQLSHKNLEALKREIEIQTRELDERRNQLMSEAKEMSYKIYLTQLEQVLQGSENGPQ
                     ITLNENNALKMILAMMKNMEEMAEKEKDIASSIEKERHNLHSLQKSLVAYTRQLEQYL
                     TSDPHLVKQNKALTEENVQLQQYSESAASWRTKAFYASLFSVGGSLISTGILSTFIIS
                     PVFFAIPGALAALGVVSFVIAVVSHCQKYFSDVQMEINDQKIEKNELVLMKQWKKANE
                     LSLTTIPGLNAKIEKSEKELYALEQKLQDQQHTMDLLLNKAQNITSNYYGSSNFFGNT
                     TGNVFYLPSAPLHEDSPLYPEIEGERVNYGY"
     gene            7368..7610
                     /locus_tag="VAH76_002359"
     CDS             7368..7610
                     /locus_tag="VAH76_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386960.1"
                     /translation="MKTVLILIIFLCSFQIQAACKCNCDPTDNKMCASQNDLEHPCPS
                     VCASPAPGMAPSFTACPVSRVTDPVTGVTRWVNMCN"
     gene            complement(7667..8416)
                     /locus_tag="VAH76_002360"
     CDS             complement(7667..8416)
                     /locus_tag="VAH76_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386961.1"
                     /translation="MSKVKAKIIAFTTVMLFLTSINIHAKGETPMNQYHQTNEATMIT
                     MKSKLPNSSEFEKFLKSGGQLVKQTEPDTKVWFALKGNDNSLMIFDAFYDSKGREAHF
                     AGQVANALKNNSEQLVLEGWENGVLKNIVNSKVLASKLPARSVQVTLANYIEFTANDG
                     KSEQLAALLSNAAEIVDKTEPQTAFWFALQLNDNTFAIFDAFSDESGQKAHFSGKVAL
                     SLKNNSKLLIKNGWEKGVLPHIKSLKVITNI"
     gene            complement(8475..9041)
                     /locus_tag="VAH76_002361"
     CDS             complement(8475..9041)
                     /locus_tag="VAH76_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO1386962.1"
                     /translation="MPMNISNTKERILAVAEALIQKDGYNAFSFKDIATAINIKTASI
                     HYHFPSKEDLGVAVISWHADKIATVLSDISNNSSLSTKEKIQKFFDAILTITYNAENK
                     MCLGGMFASDFQSLPVSIQNQAKKFFELIIEWLKGVLEADGYDNESSLSIAKQIISLI
                     EGGLLLARLYGDETFLEGVRRFIDQTIK"
     gene            9301..9528
                     /locus_tag="VAH76_002362"
     CDS             9301..9528
                     /locus_tag="VAH76_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386963.1"
                     /translation="MFFKRSNPHVTPQDLQKVIQNLNAQRELTERQLKEGSISQKTGQ
                     EEMQRLSSLIGAYQNNLMAALDDQQNTNYPK"
     gene            9860..>10105
                     /locus_tag="VAH76_002363"
     CDS             9860..>10105
                     /locus_tag="VAH76_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1386964.1"
                     /translation="MIKDILNTIASLVEENIVVFASERGKQLLQKIKADLASFKPINF
                     YALDAEKQIKDALKLIESYCIEMNRILDELNHQSSVSL"
BASE COUNT         3225 a         1794 c         1911 g         3175 t
ORIGIN      
        1 tttcttatgg cgatactttt attaatagat atactcctgt tttcagcttt acaaaggttt
       61 ctgatcaaga attcacaatt gaattggcta aaattggcga agcattttat acaattccct
      121 cagagttaag ttcttctcag gaaaaagcag cacatcttat tacacttctt actcgcgcag
      181 aagaatctca agttacagat atgcataaaa tacttaactc gtttgtttcg ggaaaaatca
      241 cgtcaggcag catgtttaat tttgaaaacg atggttcatt taagagagat cctgaggaag
      301 cttataactt aataaacaaa atttgataat ctttctaata agccagggat ggttatatgt
      361 tttctgatta ttgagactga ttttctcaat ttgcaactaa tttaagtttg tatcgacttt
      421 tctttgctta tcaaggctgt tttctttcca ggcacgaatt aaagtgcggc atgaaatttc
      481 ttcctttctg tttgacaaac agcaaaatac taaataattt taatttctta ccattttaag
      541 gaatataaaa ttatgaaagt attgtataca acaattgcca agacccatgg tggacgaaac
      601 ggccatgtag aaactacaga tgggctatta aaacttgatt tggcaatgcc gagagagcta
      661 gggggagagg gtggtgccac taatccagag caattatttg cagcgggata tgctgcttgt
      721 tttgagagtg ccattcgtca tgttgccaat atgcaaaaaa tttctttaga ggatgtttcc
      781 atgacttccg aggtttcttt atatgctact ccagagaagg gttttaaact tggagttgca
      841 ttacacgcct atataacagg cctaaatcag aataaggccg aagcattagt tgccaaagcg
      901 catgaggttt gtccttattc caatgcaata cgtggtaatg ttgaagtgaa gttgagtgtt
      961 agtgtcaagt gatagataac ttccatcaat caataaggat atcttaatgt atacacttta
     1021 ttcattcggg acgcccaacg gatttaaacc cactattatg ttggaagagt tggcacagcc
     1081 ttacaccata aaactgattg atattactca gggtgaacaa tttaatcctg attttttaaa
     1141 aatttcacca aacaataaga taccggtttt gtatgatcaa accaatgatt tttacctaat
     1201 ggaaagtgtt gctattttgc aatatttggc tgaaaaacat agacagtttt tgcctgaaac
     1261 gacaaaggag aaatatgcca ttatccaatg gtgttatttc caggcagcac acataggccc
     1321 tatgttagga caatttggtc attttcatcg ttatgctcaa gagcaagtac cctatgccat
     1381 gaagcgatat gctgatgaat ccatgcgact tctaggggtc atggaaaagc aactggccaa
     1441 aacgtcattc attggcggag caaattatac tattgccgat atggctatct ggccctggat
     1501 ttggtgtttt caattcatat atgaacagac tattgatgcg aaactgtttc ccagtttaat
     1561 gtcctggtat cagcgagtta gtgaaaggcc tgctgttcga gcgactctag ctgcctatgg
     1621 tcgattatga aagatggcaa gcaagagtag gaacctatcg gataattaat tgctcccaat
     1681 tgccgtaatg ctgccaattg gggtgcttta cttggttatt gcttcaatag attatgtcat
     1741 cttgatttaa taagggctat aacagcctag ggaaaaggta ttaatgccta acttaatttt
     1801 gctacctatt ctttaacatc ctccggaatt ttggtgttat ctatatcagt attattttta
     1861 actaaaaatg cagccagata tttaagaaag ggcgcttctt taccggtccc ggcttttctc
     1921 aaatcattaa tttagacaaa caaatgtttg taacatctcg tcttaatcct tcaaaacgag
     1981 aggctattag ctcaatgtca attgtacttg gtaactctcc atagaaatta attttttctt
     2041 taatgtgatt atcgattaaa ttggagttac gattgaaagt tgtgtacggg agtcattgaa
     2101 aagaagatag tgtggtttaa cctcattaac tgtcgaaggt taatggaaaa aatccatgga
     2161 gaagagacaa caatgggaaa cgataccgtt gtttcatgca aattctagaa gaaaaataaa
     2221 tgggatatat aattatgaaa tcaatgaaat aaaatcttcg aattttccac cagcactgcg
     2281 tggaattgaa tccacataat tgaagacaaa atgaaaggga taatcaaaga ttatactgag
     2341 tttttcccga attttttgtt cttcctggta tgccaatgaa tcagggtgtt ttaaattcac
     2401 ttgaatttca gttggagttg tttggacaag ttgaaattga gagccagaga agaggtccat
     2461 caggcgtaaa ccattgctag taaatgctgg ccatagtttt ctgccatcag gcatttgtat
     2521 catattgcgt ttcctgccca aaatgtattt tagcactggt aagtttcgcc cacaggagca
     2581 actttcgccg ggtattgcat aatcacctat atcataacga atcaggggag aggagaaatt
     2641 atgaagggtg gtaatgacaa cacgtccagg ttgatctggt ttgcacggat tattgctttc
     2701 atccaggatc tcaacaagta cgttttctga ctgcacgtga tagtacccgc ttaccgggca
     2761 ttcaagcgct atatagccgc attcttttga agagtaatta tcaattaaag gaatacctag
     2821 cacttcttta accagagacc gaagctctgg ttctacgatt tcactgtgag tatgcacctt
     2881 ttgcaggttt tcaggtttat ttccatgttt ggcaaaatac agtgtgattt ctcttaaggt
     2941 agaaggattg caattcaaat aatccggatt tatgcgttga agccattgtg cttcttgttc
     3001 tggagtgcag agagtaagtt gataacaagg tcctgtttcg acaatcgagt aggtcgcagg
     3061 actccagttt aaaaattttg ctcctaatgg tggaagagcc tccttatttt ccgtgtagcg
     3121 gatactggca aaagatttgt gaaactcatc attatgccaa agatgatttc gtagagagat
     3181 ggcattccag aaaaactggg ttgccatatt accttttacg gtgactggtt ggccagtgga
     3241 accgctggta gataataatt ccgatggctc atggcccgac ggcacttgca aggcaaaaat
     3301 attgtctttt gcaatttgaa gcgtttctct cttcaataaa gggagctctt gccaggcttt
     3361 atgcaagtct tgccactttt caaatgaggg taaataggca aattttttct gataatacgg
     3421 aacacattgc ctggaaaaac tcattaatgc caccagttgt ttaaattgat tctttataat
     3481 ttctgaaggc gggagttttt ctgtatgatt aaaattatcc agctgataat acaattgagc
     3541 tgccattgga tgtggcgcag gcggccagat aattcccgga atcgcgctgc gcaattgtga
     3601 cggtattaac ttcattttac tcaatcttaa gctcaattaa attggagcaa caattagcat
     3661 tacaagcaac cgctggtgag tttcattccg aagaataatc cttgattgct gtaattcgat
     3721 ctttgaggta gtacaaccac tccctcacca ccgaccagac ctgtcgaggg acgttcaatc
     3781 gcatcgatat aggtttcagc ttgccagccc acttgaaaat cgatagttga agcagctccg
     3841 tctcctatta aaccaaatgg tcgttggtaa tccaatccaa tgcgaagtcc cagtttaggc
     3901 actatagtgt ttctgtcaga aaaatctcta ctgacaccaa aggggtggtg cgccagcaga
     3961 gaaacatctc ttgagccaat cagtaaggct gaatttgcac ttgcaaaaac attaaactct
     4021 ctataaaaat tataaaaaac atcagcttca atacgcggac caaacccttg gaaattactg
     4081 atattagtgt gtccttgtat tgtctgttct ataccgttaa ctgttagtct tgaatcaatt
     4141 tctgcatatt ctgctccagc accataacgt ataagccagg cattattaaa atcatgcgta
     4201 tgggcaaaaa cccccgaaac ttcatcgtaa tcaaaaatat tttcgctttg tacggatgcc
     4261 catgctgcaa gattaagcca acgaggctcg atagcaatat cgccattacc caataacatg
     4321 tcgttatctc tggtatgtaa tctttgccag gctatggtga tatcgttgtt atttgcgaat
     4381 tttaccccgc catataatct aaaaccccaa tcataatcca aatgggtatt cagtgttttt
     4441 gtgtagttgt cgacgagata agttgtataa tccagatcat cattacgggg ttgaagataa
     4501 agtgcttctg ctcctgcaaa caggcgattt ccagagcagc ctgttttaac tggacccata
     4561 gttcctgcaa tagacgaatg cgtattacaa aatccaatta taaagagact taaagttgtt
     4621 ttccttaaag cagtcattaa tatcattctc ctttgataga tactcgtggt taagtcaacg
     4681 ggataatata attgataaaa atggatatta aaagcaaaaa agctctgcct taatcaagaa
     4741 ggtttttaat tggctcgtta taaattaact tgttgattta taatttaaaa atagtgagta
     4801 gatatcaacc attaagatga aggatgcacg gttgaaaagt ggactgtgga tttttttcat
     4861 cccttaccga aatcacattt taaatttagt ttattctcta agtctgttat tttggtagat
     4921 cttaacaatt ccttaacaat ctcttaacaa ttccttaaca tttgtctgcc ataatgccct
     4981 cagtttaaac aaaagaataa aatacaggag gatttatcac actgctttga atagaactag
     5041 gaaatgtagt ctgtattttc aggggaagca aatttcttct gcatcatgca gcgattcaca
     5101 ttaatagttt caataaacga gttcgttaat aatataatta ctgaggagtc acgagtcatg
     5161 gcgttatcag aaatcattat caatgatgta gttcaatatc aagccaacgc agtcattagc
     5221 gaatcaaatt accatattct tcgccaatat ctcctggaga gaaagccttt atcaagagta
     5281 ctcaatccaa tcaattctct gctttctgag cattgtgata aggataaaca agcaataata
     5341 aaccacttaa ctcaactggc ctgtgaatct caaagaaatt atgatattca agaggcaatg
     5401 agtgatgaac aagagagagt caatgaaaac tctttaatga ttaattacag gagcgagttg
     5461 cttaccctgg aaaatacatt gaagcaactg gatacaaaat gctttcaaca acaacgccaa
     5521 tacaatcaag tgagcaggca atttaatgaa cttaaagtca atctgaccca cattaatcaa
     5581 agtattgata gaatccgata tgaacgtcaa ttgcttgatc aacagctaaa tcacccttac
     5641 tcggatggta atgagtatac taatcctcct actcacacac gtatttatcc cgatctttca
     5701 ctacctcggg ctcctcttac gcgagatcga ctgctgcaag aagaaaatag attgattgag
     5761 gagcgccagc ggctgatgca tcttgtcaat tcaaaagaag ctgaaaaaga aaaagaagaa
     5821 caaaatttaa atagacttca taaggagaaa aaagagactg aagggcgtta tagtgaagta
     5881 aaacatcaaa tcgatattgc tttgccaaat agtgaacaac aacgccaaat cagaaatcaa
     5941 gagcgtcttg ctcgagaaca ggccagagca agttatgatc ctcatttatt gcaactgtct
     6001 cataaaaatc ttgaagcatt gaaacgtgag attgaaatac agacccggga actggatgag
     6061 cgacgaaatc agttaatgag cgaagcgaaa gaaatgagtt ataaaatcta tctaactcaa
     6121 ttggaacagg ttcttcaggg atcagaaaat gggcctcaaa tcacgctcaa tgaaaataat
     6181 gccttaaaga tgattttagc catgatgaaa aatatggagg aaatggcgga aaaagagaaa
     6241 gatatagcta gctcaattga aaaagagcga cataatcttc attcattgca aaaaagcctt
     6301 gtagcctaca ctaggcaact agaacagtat ctgacgtcag atcctcattt ggtgaaacaa
     6361 aacaaggctc ttactgaaga aaatgtgcag ttacagcagt atagtgaatc agcagccagc
     6421 tggagaacga aagcattcta tgcgtctctt ttcagtgttg ggggtagttt aataagtacc
     6481 ggaatattga gtacattcat aattagccct gtattttttg cgattcccgg tgctcttgct
     6541 gcgcttggtg tagtttcctt tgttatcgct gtagtctctc attgccagaa gtactttagt
     6601 gacgtgcaaa tggaaataaa tgaccaaaag attgagaaaa atgaattggt actaatgaag
     6661 caatggaaaa aggctaatga attaagttta actactattc caggcctgaa cgctaaaata
     6721 gaaaaatcag agaaagagct gtatgcgctt gagcaaaaat tacaagatca gcagcacaca
     6781 atggatctgc tgttgaataa agcgcaaaat attaccagca attattacgg aagcagcaat
     6841 ttctttggta acacgactgg taatgtgttc tatttacctt ctgcccctct gcatgaggac
     6901 tctccactgt atccagagat tgaaggtgaa agagtaaatt acggctacta ataagctaaa
     6961 atgactggtc tttaatttgg tttcaatttg aagaccagta tctctgatta ttcaaatcct
     7021 gaattgctct ttacaatctg ctttgtaaaa atgttttgat catactggat ttaaaatgaa
     7081 gtatttttga gttaatgctg tgtggtttat gagggctgga gctgatttca ggacttacga
     7141 aaaatcccaa tctctttgtt aaaaaaagtt tttaattcgg ttatttagtt tatctaaact
     7201 ccctcattaa aaacattttt tgccttgatc ttggggtttt tcgtaagtcc ttttaaaggc
     7261 agtattttga ttgcagcctc attaatgtgc taaaattgta cacatgggta acctgatatc
     7321 attgcttgaa tgctaaatta catttcctta ttgaaaagga tttccatatg aaaacagtcc
     7381 taatattaat tattttttta tgttcttttc aaatacaggc ggcatgcaaa tgcaactgtg
     7441 acccgacaga caataagatg tgtgccagtc agaatgatct ggagcatcct tgcccttcag
     7501 tttgtgcttc gccagcacct ggtatggcgc cttcgtttac agcatgccct gtatcaaggg
     7561 ttactgatcc agtcacagga gttaccagat gggtcaatat gtgtaactaa tatcaaataa
     7621 tccattattt tcagggaaga attttatctt taatttacct gactgattag atgttggtta
     7681 tcactttcag gcttttaatg tggggtaaga cgcctttttc ccatccattt ttaatcaaga
     7741 gcttagagtt attttttaaa gatagtgcga cttttcctga aaaatgagct ttctgaccag
     7801 actcatcaga gaacgcatca aaaatagcaa aggtgttatc attcagctgt aaagcaaacc
     7861 agaaagctgt ttgaggctca gttttatcaa cgatctcggc ggcattgctt aataatgcag
     7921 caagttgctc tgatttacca tcgttggcag tgaattcaat gtagttggca agagttactt
     7981 gaactgatct tgcaggtaat ttagaggcga gcactttact gtttacaata ttttttaata
     8041 cgccattttc ccaaccttca agaaccaatt gttcagaatt gtttttaaga gcattggcaa
     8101 cttgaccggc aaaatgggct tctcttcctt tagaatcata aaatgcatca aagatcatca
     8161 aggaattatc attaccctta agggcaaacc atacttttgt atcaggttca gtttgcttta
     8221 ccaattgacc gccagatttc aaaaattttt caaattcaga ggaatttggt aatttgcttt
     8281 tcattgttat catggttgct tcatttgttt gatgatattg attcataggt gtttctcctt
     8341 ttgcatgtat attgatcgat gttaaaaaca acattactgt tgtaaaagct ataatttttg
     8401 ctttgacttt actcatataa aatttcccca taatattcta tctactaata gatagttttg
     8461 gatgttaaaa aaaatcactt tattgtttga tcaataaagc gccgaactcc ttccaggaac
     8521 gtttcatctc catataatct tgctaataat aacccgcctt caatcaagga aataatttgt
     8581 tttgcgatag ataatgagga ttcattatca taaccatctg cttcaagaac tcctttaagc
     8641 cattcaatga taagttcaaa aaattttttt gcctgatttt gaattgaaac gggtaatgat
     8701 tgaaaatcag aagcgaacat acctccgagg cacattttgt tttcagcgtt gtaggtgatt
     8761 gttaaaatgg catcaaagaa tttttgaatt ttttctttgg ttgataacga tgaattattg
     8821 cttatgtcag ataacacagt agcaatttta tcggcatgcc aggatataac tgctacacca
     8881 agatcttctt ttgaaggaaa atgataatgg atgctggcag ttttaatatt gatggctgtt
     8941 gcaatatctt tgaaactaaa ggcgttatat ccgtcttttt ggatcaatgc ttccgcaact
     9001 gctaatatcc gttctttcgt attacttata ttcataggca tgaattttac ctactcatag
     9061 atagattgtc aaacttttta tagtattcta tatcaaatta aaataacact tgtttatcta
     9121 aatagaagtg ggtggtggac tgctcattca ttaattcatt cggatcttta ttgggttgat
     9181 ttatcggatc aatatcttgg tacatcgcca tgtcaattgc ccttaaagac tttatgtctt
     9241 tattgatctc gaaaaaaacg agaggtaata tatgtacata agttcacaat agaggtcatt
     9301 atgtttttta aaagatcaaa ccctcacgta acaccacaag atttacaaaa agtgattcaa
     9361 aatttaaatg cgcaacgtga attaacagag cgtcaattga aagaggggtc tatatcgcaa
     9421 aaaacagggc aagaagagat gcagcgttta tcaagcctga ttggagcata tcagaataat
     9481 ctgatggcag cattagacga tcaacaaaac actaattacc ctaagtagct aatctttttg
     9541 aggtataaaa gggcagtcat tttgattaat aatcaactca taatctgaat ggtcatttat
     9601 gtgttgatac gattttgagt acgctgtgcc attctatgta ccaagaaaac agggatgtta
     9661 tttcctgccc tatcaatgta tccgaaggct ttcccagacc tttactttaa atgatgttca
     9721 tgagtctatt taagggattg gaaataaaat agattattga aatttatgaa taattaaccc
     9781 agaaagcact tgctttattc aatctgtaca ggtatttcta ttcaatcaaa gcccattcta
     9841 ttgagtaaga gagtattaaa tgataaagga cattctcaat accatagcca gtcttgttga
     9901 ggaaaacata gtggtatttg caagtgaacg tggaaaacaa cttttacaga aaattaaggc
     9961 cgatcttgca tcgtttaaac caattaattt ttatgcattg gatgcagaaa aacaaataaa
    10021 agacgcgctt aaattaatag aaagctattg cattgaaatg aacagaatat tagatgaatt
    10081 aaatcatcaa tcttcagttt cttta
//
LOCUS       DATNSN010000124        10049 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.124, whole genome shotgun sequence.
ACCESSION   DATNSN010000124 DATNSN010000000
VERSION     DATNSN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.124"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            106..1173
                     /locus_tag="VAH76_002364"
     CDS             106..1173
                     /locus_tag="VAH76_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386965.1"
                     /translation="MNKKSILLMSMLLVSPLSMSGTVGEAQQDGKRFVFSASAIYGSL
                     SDGTLDSIPYADTIKSSGLISSGLFNVDSRWGYSLSAGYLFGPEFSYDLVLSYTNITN
                     HGSSITANSVQGDLMDNRLSLILKDFSGLQMIGPALAVVNSDYDYQNAELITHRTVPS
                     RFAKPLHYTRYYGAKATEIVKEFTARYSGESRFGFFNPPIQPLTDSINFQAKYKGIGP
                     KAGAGVYWDVNRFITIGGDISFSVLAGNSKSHLNERVEITGPEPILDFPNTGGRYAYF
                     LNHPSSLWVSPVLGANLVVASHFNLNNDSVIGVEGGIGSEQYWSMHDGERYGLANGGN
                     RLSISERFSIRNVFIKASYIV"
     gene            1323..2153
                     /gene="lpxA"
                     /locus_tag="VAH76_002365"
     CDS             1323..2153
                     /gene="lpxA"
                     /locus_tag="VAH76_002365"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEO1386966.1"
                     /translation="MQMSQLDHVHAVNHLYMLPHQIHPTAIVSANARIGRDVVIGPYS
                     IIEDNVSIGQGTVIGSHASIKSWTEIGEYNQIETGAIIGAIPQDLKFSGEKSTVIVGN
                     NNIIREYVTISRGTSGGGGVTRIGNNNVIMTSAHIAHDVQMGNHNIISNAVAVAGHVI
                     IDDWVNIGGLCGIHQFVQLGRMSMIGSQTRITKDVLPYTLVCGNPAKRFGINIERLQR
                     NGYSPVARMQIQRAYKILFHDGHLLTNAIEILKREFIDNNDVAYILKFLENSKRSFYR
                     "
     gene            2169..3200
                     /gene="lpxD"
                     /locus_tag="VAH76_002366"
     CDS             2169..3200
                     /gene="lpxD"
                     /locus_tag="VAH76_002366"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEO1386967.1"
                     /translation="MSNYQFTKPAGPFRLYELAKISGATLYEGKGETFTVSGLAKISE
                     ATSNDLVMLHQKKYVKELKNTAARSCIIGPDYVKFAPDSMYLLVHPNPYKAFALIAQA
                     FYPSEKPNSFIATSAMIESTAVIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDAVT
                     LGDDCLIEDNVSIRHAVIGNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGND
                     VEIGANTCIDRGSLDNTVIEDWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEH
                     VTLAGQAGVIGHLKIGNGATVLARGVVYKDVKPGDRVGGHPAVSISDWQKQIRFLKTA
                     IKPKKSPKS"
     gene            complement(3172..4323)
                     /gene="lpxB"
                     /locus_tag="VAH76_002367"
     CDS             complement(3172..4323)
                     /gene="lpxB"
                     /locus_tag="VAH76_002367"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEO1386968.1"
                     /translation="MKRPTRIAMVAGELSGDLLGAGVIRELKQHLTNVEFMGVGGPQM
                     LKEGFHSLIDISELSVMGISDVLRRYPQLYLIRERLLREWTINPPDVFIGIDYPDFNL
                     SVEARLKKQHIKTIHLVSPKVWAWRQKRVHLIKKAVDLVLTLFPFEEAFYRQHGVSAQ
                     FIGHPLADLIEINPSCSALRKKYNYHSDDTILAVLPGSRVGEIKYMGPLFLEVMQRIA
                     VERPHVHFIVPIACQDLYPVFFKQFYAEYGHLKIQIIQGNAREAMAISDVVLTKSGTA
                     TLEAMLLKRPMVVAFKWGRLTHAIIAPQVKVPYIALPNLLAGKKLIPEFVQEKANVDS
                     ITESVLNLLDSSNQNELIKQFTDIHHTLRQNANEKVALSILRILETSLA"
     gene            complement(4476..5333)
                     /locus_tag="VAH76_002368"
     CDS             complement(4476..5333)
                     /locus_tag="VAH76_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386969.1"
                     /translation="MFYYRAYHLNIQSDIPLGKIEQTSYCAPDVIIRKTSEIPDIPAN
                     ASRLGNKYASLKNQLWITDNQWSFYIEGGNTILYQIKSVELTTPIHILLTGACLGCLL
                     QQRNLIVLHANTVCYPGQSAFAISGHTGAGKSTAAAFHIKQGALLAADDITAIEFKQD
                     IPHVLPGFPRLKLWNDSREALHLLSLESLYSYNGRSKHHIDLKPNQHCSTPVVLEKLV
                     IIKPDLTEESELFGKEKIQCLLANNYTALIFHLFDFHWDVFKNNLKLARQLKIYNAPR
                     PTMVFYNEI"
     gene            complement(5363..7165)
                     /locus_tag="VAH76_002369"
     CDS             complement(5363..7165)
                     /locus_tag="VAH76_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1386970.1"
                     /translation="MAEFYKKYAEKYKDFLDFIRFLYEYSPFQLVFSQAMVILSSFLG
                     GVGVVSLVPMLSYSGWLAQNKSGSDLNWLSSLWQQLPMHDGQIPITFVLSVYCLIICS
                     VSMTNYLAELTKIKFMKNFERYCRESLIQNLVNARWEYLLKSKLKDSEYVLNVGLMKI
                     SALTTYTLSLFNNIIKNSVYLIVGLMLSTGLTVISLITVISIAIFFKKSNSAGMGKQE
                     FDLSRSRQINLSNFLEGIKIAKIHELTQEYLKQERDISFKVMDNNIKFLSNQMKIKFG
                     YTAIGAIAFTLIFLISISLLQISIPTLVVLLFVYNQIIRRISTMQQSMSYVLNISPLF
                     RNYLILKNDFLSNKESSNVIPFHSRVPLPKDILIQVKQVSFSYGEKAILKNVSFDIHR
                     NKITAIKGPSGVGKSTCVDLILGLLKPDDGSILFNQSVVLMDAERATSKKKWVSYVPQ
                     NIFLFNDSIRNNITWLNNDVNEADLWKVLALVDAENLVRSMPNGLNTMIGDRGVHLSG
                     GERQKLAIARAILSSPTLLILDEATSALDPQSEAVIMQSLAALKQTMTVILIAHREST
                     IDYADEIIDFGKFNLKEEYPLNFNVLEDTTVAMY"
     gene            complement(7176..7646)
                     /locus_tag="VAH76_002370"
     CDS             complement(7176..7646)
                     /locus_tag="VAH76_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEO1386971.1"
                     /translation="MAEIKKIRYAHLFLVLLGKGKTRVHVAVLIVYYLIVSRIITWLY
                     PFNRYARLFGHKHAETLYEDFDHLYWLKCCQKLFPKINRLLPWKSACLQQSVAAFLLL
                     RRKNMPTTIYFGMKRDGDEVIGHVWLRCGKRYITGGNGVGYTIFCTYALDWVRN"
     gene            complement(7704..8018)
                     /locus_tag="VAH76_002371"
     CDS             complement(7704..8018)
                     /locus_tag="VAH76_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEO1386972.1"
                     /translation="MSKHLQQHEYQFEYKTNTILERHDLVFFSSVDEDLVLLDEETSH
                     YLVVNSVGYKIWNLLESKKTVSQLVSGLMEIYKVDFDTCFNDIKAFIQRMVQHKLIKV
                     CS"
     gene            complement(8044..9870)
                     /locus_tag="VAH76_002372"
     CDS             complement(8044..9870)
                     /locus_tag="VAH76_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEO1386973.1"
                     /translation="MNALYGVLRFDGQPFDRELLERAKFNSPVWRPDHSELYYNDFYA
                     TASTQRFITPECALQTAPYHDKESGVIANGDLYLTNHSSLANWLGVSSKNSDLYLATR
                     AYLKWGEKCVNYLAGNFFLVFYDLHKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFN
                     TFKVFHDSLAVNHGAFHKLAAKQKIGQETCLKSVKKLMAARYMIVSDQNLQEQQYWEL
                     HKTQQTGQCQSREDYYSAFRNEIQQSIRQSIRTDTGIMAHYSGGLDSSSIASIAALQL
                     AKENKTLLAYTAIPRYLSGDSYRKGCKYHELDLVQSACRYHDNIEAIVYKSSEDISIF
                     DRLQPFYPYLDQPFKAVFNMEWILAGLEIAAAKNCRILLNGEQGNGSISWKGVTLGNY
                     LNQRVKFTRHFLKYHMKLGKQFIKSGSLNLKRNRLHIHEMKKNFLAPRHSLLMGKNRL
                     ETRSYMRSIYLWYGVEVIDPTSNLDLVQFCYDLPEWIYHGGNKYFEIRRLPKLKRRLL
                     VRDALEGLVPPEIRYNPYRGEQAADWYYQFNKYGHAWERLLADIAEKHPLIWEIYPKK
                     YLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFINDLNNLY"
BASE COUNT         3113 a         1960 c         1923 g         3053 t
ORIGIN      
        1 tgactcctgc gaatacgtta tttgtagctg gatatcattg aagatgtcct ttataatcca
       61 gagcgaacaa atacaatatt caaagatcag ggagtagata catagatgaa taaaaaaagc
      121 attttattga tgagcatgct cttggtaagt ccacttagca tgagtggcac agtaggagag
      181 gcgcaacagg atggtaagag atttgttttt agtgcttcag ctatttatgg ctcgttaagt
      241 gatggtactc tggattccat accatatgct gacaccataa aatcttccgg acttattagt
      301 tctggcttgt ttaatgtgga ttcacgttgg ggatattcat taagcgccgg atatcttttt
      361 gggcctgaat tcagctatga cttagtatta agctacacca atataacaaa tcatggctcc
      421 agcataactg ccaattcagt acagggagat ctcatggata acaggctaag tctgattctt
      481 aaagattttt caggactgca aatgattggc cctgcccttg ctgtggtaaa ttccgattat
      541 gactatcaaa atgcagaact gataacacac agaactgttc caagcagatt tgccaaacca
      601 ctccattaca ctcgctatta cggcgcaaaa gccacagaaa tagttaaaga atttaccgct
      661 cgctacagtg gtgaatctcg tttcggcttc tttaaccctc caatacaacc ccttacggac
      721 agcatcaatt ttcaggcaaa atataaaggg attggcccta aagcgggagc aggagtctat
      781 tgggatgtta atcgatttat cacgatcggt ggggacattt cattctctgt gttggctggg
      841 aatagtaaaa gccatttgaa tgaaagggta gaaataactg gacctgagcc gattttagac
      901 tttcccaata ccggtggccg ttacgcttac ttcctgaatc acccttcctc actatgggtt
      961 tctccagttc tcggagctaa tttggtagtg gcttctcatt tcaatttgaa taacgacagc
     1021 gtgattggtg ttgagggcgg aattggctct gagcaatact ggtcaatgca cgatggagaa
     1081 aggtatggtt tagcaaatgg aggtaataga ttaagtatta gtgagcgttt ttctatacgt
     1141 aatgtattta taaaggcaag ctatattgtt taattgaaat tcggacacgg cttgcgatta
     1201 acgcaagttg tgtcatggag tgcttttccc tctatatcaa atcattttac ccaaacggat
     1261 ttaagagtag tatagagcac gttgtaacca gcatttaatt tatagttgta gtggaggaaa
     1321 ccatgcaaat gagtcagtta gatcatgttc atgcggttaa tcatttatat atgcttcccc
     1381 atcaaattca ccctacagct attgtatcag ccaacgccag aattggacgg gatgtggtta
     1441 taggcccgta ttcaattatt gaagacaatg tcagcattgg acagggaaca gtcattggct
     1501 ctcatgcatc gataaaatcc tggacagaaa taggtgagta taatcagatt gaaacgggag
     1561 caatcattgg agcaatcccg caggatctga agttcagcgg ggaaaaaagc accgtcatag
     1621 ttggtaataa taacattatt cgcgaatacg ttacgatcag tagaggaacc tctgggggtg
     1681 gtggagtgac tcgcatagga aacaacaatg tcataatgac ttctgcccat atagctcatg
     1741 atgtccagat gggtaaccat aatatcatct ccaatgcggt agccgtagcc gggcatgtaa
     1801 tcattgacga ttgggtaaac ataggcggtt tatgcggcat acaccaattc gtacaattag
     1861 gacggatgag tatgattggc agtcaaacca gaattacaaa agacgtactg ccctacactc
     1921 tggtttgcgg aaacccagcc aagcgatttg gtattaacat tgagagattg caacgaaatg
     1981 gatacagtcc tgtcgcaaga atgcaaattc aacgagccta caagattctt tttcatgatg
     2041 gtcatctttt gacaaatgcc attgaaatac tcaagcgtga attcatagac aataatgatg
     2101 tggcttatat tctcaagttt cttgaaaatt ccaaacgcag cttttatcgc taatttttta
     2161 gagtgtgtat gtcaaactac caatttacaa aacctgcagg accatttcgt ttatacgaat
     2221 tagctaagat ttctggtgct acactgtatg aaggtaaagg agaaaccttt acggtttctg
     2281 gtttggcaaa aatttcagaa gccacaagca atgatttggt aatgttgcat caaaaaaaat
     2341 acgtcaaaga attaaaaaat actgctgctc gatcttgtat cataggccca gactatgtga
     2401 aatttgcccc tgatagcatg tatttactgg tgcacccaaa cccgtataaa gcctttgctt
     2461 tgattgctca agcgttttat ccttctgaaa aaccaaacag tttcattgcc acttcggcaa
     2521 tgattgaatc gaccgctgtt attggcgtgg attgttttat tgctcatggg gcttatattg
     2581 gtaatcaggt taaaattggc aacagatgca aaattggagt gaatacttat attggcgatg
     2641 ctgtaacctt aggcgatgat tgtcttatag aagacaacgt cagcattagg catgctgtta
     2701 tagggaataa cgtggtcatt tattccggtg ctcgtatagg ccaggatggt tttggctttg
     2761 ccagtgatgc gaatggccat tataaaattc cccatgcggg tggtgtcatt attggcaatg
     2821 atgttgaaat cggggctaat acctgcattg accgaggctc cctagataat acggtgattg
     2881 aggattggtg tcgtctggat aatcttgtgc aaattgggca taatgtcaag attggtaaag
     2941 gctctgtcct agtggctcaa gttgggatcg ctggcagcac agagcttggt gagcatgtga
     3001 ctctggccgg gcaagctggc gtgattgggc acctgaaaat tggaaacggg gcaacggttc
     3061 ttgctagagg tgtagtctac aaagatgtaa aacctgggga cagggtcggc ggccacccgg
     3121 cagtatcaat tagtgattgg caaaaacaaa tccgattttt aaaaacagcg attaagccaa
     3181 agaagtctcc aaaatcctga ggatagataa agccactttc tcattagcat tttgccttaa
     3241 tgtgtggtga atatcagtaa attgtttaat tagttcgttt tgattggatg agtcgagtaa
     3301 attcagaact gattctgtaa tgctatcaac attcgctttt tcctgaacaa attcagggat
     3361 taatttttta ccagccaata aattaggtaa cgcaatatag ggaactttta cttgcggcgc
     3421 gataatcgca tgagtaagtc taccccattt aaaggcaacc accatgggtc gttttaaaag
     3481 catcgcctca agtgttgctg ttccggattt tgttaaaaca acgtcagaga tagccatggc
     3541 ctcacgagcg ttcccttgaa taatctgaat ttttaagtgg ccatactcgg catagaattg
     3601 tttaaaaaaa acaggataca aatcctgaca cgcaatcggt actatgaaat gcacatgagg
     3661 cctctccaca gcaattcttt gcattacttc caaaaacaag ggccccatgt atttgatttc
     3721 acctactcga ctacccggta atactgccag aatggtgtca tcagaatggt agttatattt
     3781 tttacgtaaa gcagaacaac ttgggtttat ttcgattaaa tcagctaaag gatgaccaat
     3841 aaattgtgca gataccccat gttgtcggta aaatgcttct tcaaatggaa atagggttaa
     3901 tactaaatca actgccttct ttattaaatg aacccttttt tgtcgccatg cccaaacttt
     3961 ggggctcacc agatgaatgg ttttaatatg ttgttttttt aaccgcgctt caacggataa
     4021 attaaagtca gggtaatcaa tcccaatgaa tacatctggt ggattaattg tccattccct
     4081 caataacctt tctcttatta aatacagttg aggatatcgt ctcagaacat cactgattcc
     4141 catcacagaa agctcggaga tatcaatcag ggagtggaaa ccttccttga gcatttgagg
     4201 accaccgaca cccatgaatt ctacgtttgt taaatgctgt ttcagctcac gaattacccc
     4261 tgctcccagt aagtctccag atagttcgcc ggcaaccatc gcaattcgtg tcggtctttt
     4321 catagtcaaa ttgcaccaat ataaaattta aatgactcga tgttagccta aaccaataac
     4381 catagccaag cagtattaat cccgcaaaca ctcagattgc caattaagta tcagaaatcg
     4441 ctttacaaat aatttttcag tctgccaaag atcatctaga tctcattgta aaataccata
     4501 gtcgggcgag gggcattgta tattttaagt tgtctggcta attttaaatt attcttaaat
     4561 acgtcccaat gaaaatcaaa tagatgaaat atcagagcag tgtagttatt tgcaaggaga
     4621 cattgaatct tctctttacc aaagagttca ctttcctctg tcaaatcagg ttttataata
     4681 accaattttt caagcactac tggtgtgctg caatgttgat taggttttaa atcaatatga
     4741 tgcttggatc ttccattata agaatagagt gactccaaag ataataaatg tagggcttct
     4801 ctactatcgt tccataattt caaacgagga aatcccggca atacatgagg gatatcctgt
     4861 ttgaactcta tggctgttat atcatctgcg gccaataaag cgccttgttt tatatgaaat
     4921 gcagctgccg tagattttcc cgccccggta tggccagaga tggcaaaagc ggattgacct
     4981 gggtagcaaa ctgtattggc atgcaaaaca atcaaatttc tctgctggag caggcagccc
     5041 aaacaggcgc cagtaagaag aatatgaatt ggtgtagtca actcgacgga tttaatctgg
     5101 tataaaattg tgtttccccc ttcaatataa aagctccatt ggttatcggt aatccatagc
     5161 tgatttttta aagacgcgta tttgtttccc aagcgactcg cgtttgcagg tatatcagga
     5221 atttcactgg ttttacgaat aatgacatct ggagcacaat aagaagtttg ctctattttt
     5281 ccaagtggaa tgtcactttg tatgttcaag tggtatgcgc gatagtaaaa cataaacgtc
     5341 tttataaaaa attgataaat aattaataca tagcaaccgt agtgtcttca agaacattga
     5401 aatttaacgg atattcctcc tttaaattaa acttgccaaa atcgattatt tcatccgcat
     5461 aatctatggt actttcccga tgagctatta aaatgactgt catggtctgt tttaaagcag
     5521 caagcgactg cataataaca gcctcgcttt ggggatcaag tgcactggtt gcctcatcga
     5581 gaataagcag cgttggcgat gacagtatgg ctctggcaat cgccagcttc tgccgctctc
     5641 cgccagataa atgcactcct ctgtcgccaa tcatggtatt caatccattg ggcatagacc
     5701 taaccaggtt ttcagcatca accagggcaa gaactttcca taaatctgct tcattcacat
     5761 cattgtttag ccacgtgatg ttatttctga ttgaatcatt aaaaagaaaa atattttgag
     5821 gaacataact aacccatttt ttcttggaag ttgcacgttc cgcatccatt agaactacgg
     5881 actggttaaa gagaattgag ccatcatccg gcttcaacaa gcccaatatt aaatcgacac
     5941 aggtggattt cccaacacca gatgggcctt ttattgcagt tattttattt cgatgtatgt
     6001 caaatgaaac attttttaaa atagcttttt ctccatagga gaaggaaact tgctttactt
     6061 gaattaatat atccttaggt aatggtactc gcgaatgaaa gggtatcaca tttgatgatt
     6121 ctttattcga taaaaaatca tttttaagga ttaaatagtt tcgaaacaag ggagaaatat
     6181 tcaatacata agacatgctt tgctgcatcg ttgatattct tcttatgatt tgattgtaaa
     6241 caaataataa aacaaccaac gttggaattg aaatctgtaa cagggaaatg gaaataagaa
     6301 aaattaaagt gaacgcgatc gctccgattg cagtatatcc aaatttaatt ttcatctggt
     6361 tacttaaaaa tttaatgtta ttgtccatca ctttgaatga gatatcacgc tcttgtttga
     6421 gatattcctg tgtaagctca tgaattttag ctatttttat tccttcaaga aaatttgaca
     6481 aattgatttg cctgcttctg ctcagatcaa attcttgttt tcccatacca gcagaatttg
     6541 attttttaaa aaaaattgct atcgaaatca cagtaattag cgaaatgact gttaatcccg
     6601 tagaaagcat taaaccaaca atcaaataaa ccgagttttt gattatgtta ttaaataaac
     6661 tcaacgtata agttgttaaa gcagagattt tcatcaaacc aacattcaat acatactcag
     6721 aatctttcag tttgcttttt agcaggtatt cccatcgcgc attcaccaag ttttggatta
     6781 agctttcacg gcagtatctc tcaaaatttt tcataaattt tatttttgtt aattcagcga
     6841 gataattggt catggagact gagcatatga tgaggcaata aaccgataag acaaaagtga
     6901 ttggaatttg gccatcgtgc atcggcagtt gttgccataa actggataac caattgagat
     6961 cagagccgga cttattttgg gctagccaac cactatagct taacataggc accaatgaga
     7021 ccactccaac accgcccaga aagcttgaaa gaattaccat tgcttgtgaa aacacaagct
     7081 ggaacggcga atattcatat aaaaacctga tgaaatcgag aaagtcttta tatttttccg
     7141 catatttttt ataaaattca gccataaaac aacacttaat ttcttaccca gtctaatgca
     7201 taagtacaaa aaatagtata acccacaccg tttcctccag tgatatagcg tttaccgcac
     7261 cgcagccata cgtgccctat gacctcatct ccgtctcgtt tcataccaaa ataaatcgtt
     7321 gtaggcatat ttttgcgacg taatagcaaa aatgccgcaa cactttgctg taaacaggcg
     7381 cttttccatg gcaacaatct atttatttta ggaaacagtt tttggcaaca ttttagccaa
     7441 tacaaatggt caaagtcttc atacaaagtt tcggcgtgtt tatgcccaaa caacctggca
     7501 tatctgttaa acggatataa ccaggtaatg attcttgata ctattaggta ataaacaata
     7561 agcacagcaa catgaactcg tgtttttccc ttgcccaata aaaccagaaa aagatgggca
     7621 tatcttattt tctttatttc agccaattgc attatccgat tctgcttaaa aatgatgaca
     7681 gtgaatgatt ataaattctt tatctatgaa cagactttga ttaatttatg ttgaaccatt
     7741 cgttgtataa acgctttaat atcattaaag caggtatcga aatcaacctt atatatttcc
     7801 ataagcccag aaacaagttg gctcaccgtt ttttttgatt ccagcaaatt ccatattttg
     7861 taacctacgg aattaactac caaataatga cttgtttctt catcaagcag gaccaaatcc
     7921 tcatcaacgc tggaaaaaaa caccaaatca tggcgttcta gtattgtatt agttttatat
     7981 tcgaattgat attcgtgttg ttgtaaatgc ttgctcatcg agacaatctc cagtaaatat
     8041 tagtcaatac aggttattta ggtcattgat aaaaaatgct gtgcttaaag cagggatcaa
     8101 ctgcgcttca acatcttgaa aatgccttga ttgcggatta atgggatgat tagcaaataa
     8161 atccaaaagg tattttttag gataaatttc ccaaatgagt gggtgtttct ctgcaatatc
     8221 tgccaaaagc ctttcccaag catgaccata tttattgaat tgatagtacc aatctgctgc
     8281 ctgttcaccg cgatagggat tatagcggat ctcagggggc acaagccctt ccaaagcatc
     8341 tctcaccaat aagcgccttt ttaatttggg caatcgcctg atttcaaaat atttgttacc
     8401 tccatgataa atccattcag gaagatcata gcaaaactgg accagatcca ggttacttgt
     8461 tgggtcaatc acctctaccc cataccacaa ataaatggaa cgcatgtaac ttcttgtttc
     8521 caatctgttc ttacccatca agagagaatg acggggagct aaaaaatttt ttttcatttc
     8581 atggatatgg aggcgatttc ttttcaggtt tagcgagcca gattttataa attgcttgcc
     8641 taatttcata tggtatttta aaaaatgccg cgtaaatttt acacgctgat tcagatagtt
     8701 gcccaatgtc actcccttcc atgaaatgga gccattcccc tgctcaccat ttaaaagaat
     8761 cctgcagttt ttggctgcgg ctatttccaa tcccgctaat atccattcca tattgaatac
     8821 cgctttgaat ggctgatcca aataaggata aaaaggttgc aatctatcaa aaatgcttat
     8881 gtcttcagaa cttttataga ctatagcctc aatattatca tggtaacggc acgcgctttg
     8941 caccagatct aattcatgat atttacatcc tttccgataa ctatcacctg acaagtatct
     9001 gggtattgcg gtataagcca aaagagtttt attttctttg gcaagttgca atgccgcaat
     9061 cgaagcaatg gatgaagaat ctaaaccacc gctgtagtga gccatgattc cagtgtcagt
     9121 ccggatactt tgcctgatac tttgctgaat ctcatttctg aatgcactat agtagtcttc
     9181 acgcgattgg cattgtcctg tctgttgtgt cttatgaagc tcccagtatt gttgctcttg
     9241 taaattttgg tcgctaacaa tcatatatct ggcagccatc agttttttaa ccgatttcag
     9301 gcaagtttcc tgacctattt tctgttttgc cgcaagctta tggaaagcgc catgattcac
     9361 cgctaaggag tcatgaaaaa ctttgaatgt attgaatgta ttgctaaata taaagtattt
     9421 accgggtaca aaaagatagt aacagcaaca aaaattaaaa tgatcaatgg caataaacag
     9481 ccgttttttg tgtaagtcat aaaatacaag gaaaaagttc cctgctaaat aattcacaca
     9541 tttttcaccc cattttaaat aggcccgggt tgccaggtat aaatccgaat ttttggagct
     9601 aactcctaac caatttgcca aactggaatg attagttaga taaagatcgc cgttggcaat
     9661 tacacctgat tccttatcat gatatggagc tgtttgtaag gcgcattcgg gagtaataaa
     9721 gcgctgtgta ctcgccgtag cataaaaatc attgtaataa agttcgctat gatccggtct
     9781 ccaaaccggg ctattaaatt tggctcgttc taataactct cgatcaaatg gttgcccgtc
     9841 aaaacgcaga actccataca aagcattcat gctacatcct aagataaaaa agaaaaataa
     9901 ctactcatga gtatgttgtt taatttctta accaaccaag aaatataatc acaacaacag
     9961 caaaatcagt gtattcgtag ttggtagaac aaactccagc tccaatctat gctgttcatt
    10021 aaatataata aaattcatta gccaccatg
//
LOCUS       DATNSN010000125         9910 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.125, whole genome shotgun sequence.
ACCESSION   DATNSN010000125 DATNSN010000000
VERSION     DATNSN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.125"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            70..1209
                     /gene="dnaJ"
                     /locus_tag="VAH76_002373"
     CDS             70..1209
                     /gene="dnaJ"
                     /locus_tag="VAH76_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO1386974.1"
                     /translation="MEQRDYYELLEVSRNASDAEIKKAYRRLAMKYHPDRNPGDTSAE
                     EKFKEIQKAYNILSDKQKRAAYDQFGHAGVDPSMGGGPGGFGGFGGFGDVFEDIFENI
                     FSGGRGHGRQSRGQRGADLQFNVQLTLEEAAIGKEVEITVPRHGTCTVCEGSGAKKGT
                     SPKTCETCQGMGQVRIQQGFFSIQQTCPTCHGEGKIISDPCASCHGQGRVRESKKINV
                     KIPAGVDNGDRVRLSGEGEAGVHGGGSGDLYVQISLKKHAIFERHENDLHCEVPISFA
                     TAALGGSIEVPTLEGRVTLKIPAETQTGKVFRLRSKGMKSVRGYGQGDLLCKVVVETP
                     VNLSREQKELLNKLQDSLENAKGTHSPKTSSWFAGAKKFFEDMKF"
     gene            1305..2417
                     /gene="carA"
                     /locus_tag="VAH76_002374"
     CDS             1305..2417
                     /gene="carA"
                     /locus_tag="VAH76_002374"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO1386975.1"
                     /translation="MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEML
                     TDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAGLIMRNCTAIPSNYRAEQSFPDW
                     LSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVAKAKSFAGLQGADLAL
                     EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTS
                     AEEVLAMNPNGVFLSNGPGDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGI
                     TKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQG
                     IRHKTKPAFGFQGHPEASPGPHDIDILFNEFVKLMK"
     gene            2626..3774
                     /locus_tag="VAH76_002375"
     CDS             2626..3774
                     /locus_tag="VAH76_002375"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO1386976.1"
                     /translation="MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACE
                     VLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSP
                     DIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
                     WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWL
                     NDKTRYFINPTGRFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSA
                     AYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTH
                     FDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL"
     gene            3793..5118
                     /locus_tag="VAH76_002376"
     CDS             3793..5118
                     /locus_tag="VAH76_002376"
                     /EC_number="3.13.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEO1386977.1"
                     /translation="MELVEEGVQKINTLQDYKVADISLADWGRKEIAIAETEMPGLMA
                     LREEFSARKPLQGARIAGCLHMTIQTAVLIETLVALGAEVRWSSCNIFSTQDHAASAI
                     AAKGIPVFAWKGETEEEYWWCVEQTLSGPNGWTPNLLLDDGGDLTQVVHQKHPKLLTA
                     IKGVSEETTTGVARLYEMAKHGQLKIPAINVNNAVTKSKFDNLYGCRESLLDGLKRAT
                     DVMIAGKVALILGYGDVGKGCAQALRGQGATVLVAEIDPICALQAAMEGYRVVTLDDV
                     AEQVDIVVTATGNYHVVTHDHMKRMRNQAILCNIGHFDSEIDIQSLKQYQWENIKPQV
                     DHVIFPDGKRIIILAEGRLVNLGCATGHPSFVMSASFTNQVLAQIELFQNSSQYQNQV
                     YVLPKVLDEKVARLHLGRIGAKLTQLSKEQADYIGVDKNGPYKPDHYRY"
     gene            5242..6249
                     /locus_tag="VAH76_002377"
     CDS             5242..6249
                     /locus_tag="VAH76_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO1386978.1"
                     /translation="MDLVIAANYLSTLEKCLEQFGINHFLEKIGFDLVRVHDPDAYLT
                     QDEFNQVILEAYKLSNCPELGLVFGQNLSIVNHGFLGYAAMSSPTFGTAIQTVLSYLN
                     TRTNLLSLELRQFNNENSAYIKLLPHTKEMVLSRFITELAVTHLIKMRNFLISCNDPL
                     LKIELNYDEPPYAKYYQTIFQTKIVFNTEATRVWFKAEELNYPIHFADDVSYQLAKRQ
                     LQELEKKMSLKEDMASKIKSILINQNLNHASMDEIASRLCMTSRTLRRHLQRLDVTYQ
                     ELLDEVREQKAKTFLLNNNISMTEISFLLGFQDTSNFSKAFKRWTGVTPTEYREKHEV
                     Q"
     gene            6381..8069
                     /locus_tag="VAH76_002378"
     CDS             6381..8069
                     /locus_tag="VAH76_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO1386979.1"
                     /translation="MGLKSGILGLSLLSLACGSFAMTETETIRVIIKYKQQPATMASL
                     KSQVIKSVQIPVKEMKPMANGAYSLIFDTSNSHAIVKAKEEDVTAKVLERLRKNPNVL
                     YAVKDRIGHFKPLPDPVINQELSQLLSHESQWDEFERPAGIRLESKPGLRDGAWAYTT
                     GKSRKPIVVAVLDTGIALNDSLVNNLVKDNEGNVFGWNFAGNNNDLSDETRSYHGTHV
                     AGTIAGYGDVMNGVGEDLKILTVKIPAENGMFYESAVVNSIYWAVGGDVPGVPKNIHP
                     AKVLNMSFGVDLEPGKEIDYCDEALQEALFFARKKGAVVAVAAGNDNVWEHYNAPAVC
                     NGTIKVASTGPEGLRSYFSNYGPSITLAAPGGDKRYGTSGGILSTVNPGGGYNSSGFD
                     FYQGTSMASPHVAGVAGLIFAVSNKALKPEQVEQILYVTTHEFGQSNDTNKSCVGKKP
                     CGHGILDADNAVQAALNGYDVLFSAPKMIALAMHECGENRLKPGKNIINDSTATWIQI
                     KSSCETGVSYQKPRIEKAKDGKIYAYYGSVSYELDQSAYKECRVVGYDGVGCYL"
     gene            complement(8223..8504)
                     /locus_tag="VAH76_002379"
     CDS             complement(8223..8504)
                     /locus_tag="VAH76_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386980.1"
                     /translation="MAETIDDITIAFNENGVETTRELDKQILSKGAWTTIMFKYQDWD
                     NTKNDYGPVKYSIRRYQKRNNQYWQKSKFNISSTEQAKKIVDILNNWLE"
     gene            complement(8518..9132)
                     /locus_tag="VAH76_002380"
     CDS             complement(8518..9132)
                     /locus_tag="VAH76_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEO1386981.1"
                     /translation="MEKAMKSILLMSLLLLIMPFRLMSAQSLYCPQNHGYINIGMSMD
                     QVIAACGQPLSKQESNQPILQKIPVQQLIYNNLGNSDGLYSGSLNVPEGTAFYGVWNI
                     PTGSSGVQLEVDILNNKVQDIRVNGGNTNAFSLCGNASIQIGDPATKVYGACGSPNLV
                     NNTYINQVVPTETKPQIWIYKPGPYQPAFSLTFVDGKLQSIGNN"
     gene            complement(9180..9755)
                     /locus_tag="VAH76_002381"
     CDS             complement(9180..9755)
                     /locus_tag="VAH76_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO1386982.1"
                     /translation="MAGLTAVGYFLLSLLFTIVIFSLWLRIAIRFFRISAINPFSQLI
                     HTITDPLVKPLYWIIKKPLQPGQKYDWIAFGVLILVEFFKIISLSLLMYHAIIPFLFI
                     FLYILVDLIIQPCEILFFAVLIRVIMSYVNPKWQHPLGDFLRIITTPILRLGRIIVPD
                     ISGFDFSPFIIMVILKIITLFLSASLPWKLL"
BASE COUNT         3060 a         1679 c         2183 g         2988 t
ORIGIN      
        1 agctaattta gagtcttcat acgactgaga gaattataat taagtgattg ttataaattg
       61 tgagcagtta tggaacagcg ggattattat gaacttttag aagtaagtcg aaatgcgagt
      121 gatgcagaga ttaaaaaagc atatcgcagg ctggcaatga aataccatcc cgatcggaat
      181 ccaggtgata cctctgccga agaaaaattt aaagaaattc aaaaagccta taatatcttg
      241 tccgataagc aaaaacgcgc cgcttatgat caattcggcc atgccggagt ggatccttca
      301 atgggtggcg gcccaggtgg ttttggcgga tttggaggat ttggtgacgt ttttgaagat
      361 atttttgaga atattttttc aggggggcgt gggcatgggc gtcaatcacg tggacaaaga
      421 ggcgctgatt tgcaatttaa tgtgcaattg acccttgaag aagcggctat aggaaaagaa
      481 gttgaaatta ctgttccaag acatggtact tgtactgttt gtgagggatc gggggctaaa
      541 aaaggcacaa gcccaaaaac ttgtgaaaca tgccaaggca tggggcaagt cagaattcaa
      601 caaggttttt tttccataca acaaacctgc cccacttgtc atggtgaggg aaaaataatt
      661 tctgatcctt gtgccagttg tcatggtcaa ggaagagtta gagaaagtaa aaaaataaat
      721 gtcaaaatcc cggctggggt cgataatggt gatcgggtcc gtttaagcgg agaaggtgaa
      781 gcaggggtgc atggtggtgg ttcaggagat ttatatgttc aaattagctt gaaaaaacat
      841 gctatttttg agcgccatga aaatgatttg cattgtgaag tacctattag ttttgcaact
      901 gcggcgttag gaggctcaat cgaggtaccc actttggaag gccgagtcac tttaaaaatt
      961 ccagcggaaa ctcaaacagg caaagtgttc cgcttgagaa gtaaagggat gaaatctgtc
     1021 agaggatatg gacaaggcga tttgctatgc aaggtagttg tagaaacacc ggtcaattta
     1081 tctcgagagc aaaaagaact gttaaataag ttacaggatt cattagagaa tgccaaggga
     1141 acgcactcac ctaaaacaag ctcctggttt gctggtgcga aaaaattctt cgaagacatg
     1201 aaattttgac ttaattaagt tacaattgga agatttgtcg ttttcagcat ggattgccta
     1261 ttttacgctg gataagcata ctttgaacat aattgagtat attcatgatt aataaatcag
     1321 caattttagc tttagctgat ggcactgttt atgagggcct gtcagtaggg gccactggtg
     1381 attctgttgg tgagttggtt tttaacacgt caatgactgg ctatcaggaa atgcttactg
     1441 atccttcgta tgccagacaa ataattacct taactactgc tcatgtggga aatacgggtt
     1501 gtaacatgga ggacatggag tccacaaaag tctgggcagc cggcctcatc atgagaaact
     1561 gcactgcaat ccctagcaat tacagagctg aacaatcctt cccagactgg ttaagtaaaa
     1621 atggagttgt tgcaatttct ggcattgata cgcgcgcgct tacactgaaa ttgcgtgaga
     1681 agggttctat cggagcttgt attagcacca atgtggagaa acctgaaatt gctgtggcaa
     1741 aggctaaatc ctttgcaggg cttcagggtg cggatttggc tttagaggtt acaagacaaa
     1801 cgatagagcg ttggcatgaa ggtcggggaa tgtggagtct agacaaacag cctcaacagc
     1861 ttcatgttgt tgcctatgat tttggtgtga aacataatat tttacgtatc ttacatgata
     1921 agggatgcca tattactata gtccctgcta aaacttcagc agaagaggtt ttagccatga
     1981 atcctaatgg tgtcttttta tccaatggac cgggtgatcc ccaagcatgc gattatgcca
     2041 tcaaagcaac acaggtattt ttagacaata atattccttt atttggtata tgccttgggt
     2101 ttcagatttt ggctttggca tgtggtggta ttactaaaaa aatgaaattt ggacatcatg
     2161 gttccaatca tcctgtagtt gaaacaactg gcaaaaaacg tgtttttata acaagtcaaa
     2221 atcatggttt tgctgtagat gaggattcat tgcccaattg cctggaagtt acgcatcgtt
     2281 cattatttga taacagttta cagggaataa ggcacaaaac aaaaccggca tttggttttc
     2341 aaggccatcc agaagcaagc cctgggccac atgatataga tattttattt aatgaatttg
     2401 taaagctgat gaaataaatt tttagcttca gaattattat tttggcaaga gtggcttgtt
     2461 ttaagcctgc ttacaatgat tgtaataccc ctaaatgcta tccatggcta ttaaagatta
     2521 gtaaaatgac atggatttac caaaacaata actataactt gttttgtgtt ttaagcagag
     2581 ataaagatta gggatactaa aaatagaaaa tacagaggat atgtaatgaa tgaagtctat
     2641 gtttttacct cagagtctgt ttccgagggc catcctgata agatagctga tcaaatttca
     2701 gatgccattt tagatgctat tttagcccaa gatccaaaag cacgagtggc ttgtgaggta
     2761 ttggttaaaa ccggtatggt tttggttggt ggagaaatta cgacgaaagc ttgggttgat
     2821 gttgaagaaa taactcgtca tgttatcaag gacattggct ataatagttc ccagatgggg
     2881 tttgattggg aatcttgtgc tgtgctttca gcgattggca agcaatcacc agacatagct
     2941 caaggtgtcg ataatcagca aactaaaatt ctgggtgctg gcgatcaggg acttatgttt
     3001 ggttatgcca gccgtgaaac agatgttttt atgcctgccc ctattgccta tgcgcatcgt
     3061 ttgatggaga aattagctaa agctcgtaaa tcaggccaat tgccctggtt aagacctgat
     3121 gcaaaatgtc aacttacttt gaaatatgaa caagggatgc ccgttgaagt ggatactgtt
     3181 gttttttcta cgcagcattc acctgatatt gagcataaag atttggttga ggcgatccgt
     3241 gaagaaatta ttaaatcagt tcttcctgct gaatggctca atgataagac ccggtatttc
     3301 attaatccaa caggacgctt cgtaattggt ggtcctttgg gcgattgtgg cctaactggc
     3361 cgtaaaatca ttgttgatac ttatggtggt atggcaagac atggcggagg ctgtttttca
     3421 ggtaaagatc cttcgaaggt agatcgctcc gcagcatacg ccgcaagaca tgttgccaag
     3481 aatattgtgg cagctggttt ggcagataaa tgcgagttac agatttccta tgctattggt
     3541 gttgctgaac ctacatctat ttttgttgat acttttggta ctggacgact aaaaaacagc
     3601 gaaataattg atttaattca tacgcatttt gatttaacac cgcaaggtat tattgatcaa
     3661 cacgatttgc ttcgtcctat ttataggcag acggcaactt atgggcacta tggcagggag
     3721 aattttccat gggagcgatt ggacaaagta gctgaattaa gcaaggcatt ataatttttt
     3781 aaggatactc taatggaact agtagaagaa ggagtacaga aaatcaatac attacaggat
     3841 tataaagtag cagatatttc attagcggac tgggggcgaa aagaaatagc aatagctgaa
     3901 accgaaatgc cgggtttaat ggctttacgc gaggaatttt ctgcgagaaa accacttcaa
     3961 ggcgctcgga ttgcaggttg tctgcatatg acgattcaaa ccgcagtatt gattgagacc
     4021 ctggttgcat tgggtgctga agtccgctgg tcttcctgca atattttttc tactcaggat
     4081 catgcagctt ccgctattgc tgcaaaagga atccctgtct ttgcctggaa gggcgaaaca
     4141 gaagaagaat attggtggtg tgttgagcaa actttaagtg gccccaatgg ttggacacct
     4201 aatttattat tagatgatgg tggagatctg acacaagttg ttcaccagaa acatcccaaa
     4261 ttactgactg caattaaggg agtatctgag gaaaccacta caggtgtagc caggttgtac
     4321 gaaatggcga aacatggaca attaaaaatt ccagccatta acgttaataa tgcggtgacc
     4381 aaatccaaat ttgataattt gtatggatgt cgggaatccc tgcttgatgg gttaaaacgt
     4441 gcaacagatg ttatgatagc tggtaaggtt gccttgattc taggctatgg tgacgtaggg
     4501 aaaggttgcg ctcaggcctt gcgtgggcag ggagccacag tacttgtcgc agaaattgat
     4561 cctatttgcg cacttcaggc agcaatggaa ggctacagag tcgtcactct ggatgatgtt
     4621 gctgaacaag tggatatcgt agttacagct actggcaatt accacgttgt aactcacgat
     4681 cacatgaaac gtatgcgaaa tcaggcgata ttgtgcaaca taggccattt tgattcggaa
     4741 attgatattc aaagcttaaa acagtatcaa tgggaaaata tcaagccgca ggttgatcat
     4801 gtgatttttc ctgatggaaa gcggattatt atcctggctg aagggcgttt agtgaatttg
     4861 ggctgcgcta caggccatcc aagttttgtt atgtctgctt cctttaccaa tcaagtattg
     4921 gcgcagatcg agctgttcca aaactctagc caataccaga atcaagtata cgtacttcca
     4981 aaagttttgg atgaaaaagt agcccgtttg catttggggc gtattggtgc gaaattaact
     5041 caattaagta aagagcaggc tgattatatc ggcgtcgata aaaatggacc ttacaagccg
     5101 gatcattatc gttattaaac taactttatt caagatttag tcaagacaac taaatcttga
     5161 atttttttac attattggat cttatactct aatatagatt agaattaatt cacttctcac
     5221 ttaaaaaaga aaagcctgtt gatggatctg gttatagcgg caaattatct ttctacactg
     5281 gaaaaatgtc ttgaacaatt tgggattaac cattttttag aaaaaattgg ctttgatctg
     5341 gtaagagtac atgatcctga tgcctactta actcaggatg aatttaacca ggttattttg
     5401 gaagcttata aattaagcaa ttgcccggaa ttgggtttgg tttttggtca aaacttatct
     5461 atagtcaatc acggttttct tgggtatgca gcaatgagca gtcctacatt tggaactgct
     5521 atacagactg tgctcagtta ccttaataca cgaaccaatt tgttgtctct ggagttacgc
     5581 cagtttaata atgaaaacag tgcttatatc aagttattac cacacacaaa agaaatggtt
     5641 ttatcacgtt ttataactga attggcagtt actcatctta ttaaaatgag aaattttttg
     5701 attagttgta atgacccgtt gctcaagatt gagttaaatt acgatgaacc tccttatgca
     5761 aaatattatc aaacaatttt tcagactaaa attgttttta atacagaggc tactcgcgtt
     5821 tggtttaaag cggaggaact gaattacccc atccattttg ctgacgatgt cagctatcag
     5881 ctggcaaaaa ggcaattgca agaacttgaa aaaaaaatgt cattaaaaga ggacatggca
     5941 agcaagatta aatctatttt aatcaatcag aatttaaatc atgccagcat ggatgagata
     6001 gccagtcgat tatgcatgac ttctcgtact ctaagacgtc atttgcagag attggatgtg
     6061 acttaccaag agttgcttga tgaggtaaga gaacaaaaag caaagacatt tttgctcaat
     6121 aataatattt ccatgaccga gatcagtttt ttattgggtt ttcaagatac atccaatttt
     6181 tcaaaagcat tcaaacgatg gacaggagtc actcccacag aatataggga aaaacatgaa
     6241 gtccaatgat tgctccattt taatttaatc tcatggctta ttgattcttg agttgatatt
     6301 tattctcctg catactgagg tggttttatt tttaaaaatt ggtcaaggct acggattgac
     6361 tttaaaaagg agattgaagt atgggtttga aatcaggaat attgggatta agtcttttat
     6421 cgcttgcttg tggcagtttt gctatgactg aaactgaaac aatcagagtt atcattaaat
     6481 ataaacagca accagccacc atggcttcac taaaatcaca agtcattaaa tctgttcaaa
     6541 tacccgttaa agaaatgaag cctatggcca atggcgctta ttcacttatt tttgatacat
     6601 cgaattccca tgctattgtc aaggctaaag aagaagatgt tacagccaaa gttttagaac
     6661 gtttgcgcaa aaatcccaat gtactttatg cagttaaaga cagaatagga cattttaagc
     6721 ctctccctga tccagtaata aaccaggagt taagtcaatt gttatcccat gagagtcaat
     6781 gggatgaatt cgaaaggcca gcgggaattc gtctggaatc caagcctggt ttaagagatg
     6841 gcgcatgggc atatactaca ggaaagtcca ggaagccgat agttgttgcg gttttagata
     6901 ctgggattgc attgaatgac agtttagtga ataaccttgt aaaggataat gaaggtaatg
     6961 tatttggatg gaattttgcc gggaataata atgatttatc tgatgaaacc agatcctacc
     7021 atggcaccca tgttgccggt accattgccg gttatggcga tgtcatgaac ggagttggtg
     7081 aggatttaaa aattctgact gttaaaattc ctgccgagaa tggtatgttt tatgaaagtg
     7141 ctgtggtgaa tagtatctat tgggctgttg gaggtgatgt tccgggagtt cctaaaaata
     7201 tacatccagc caaggtatta aatatgagtt ttggtgtcga tttagagcct gggaaagaaa
     7261 ttgattattg cgacgaagct ttacaagagg ctttattctt tgctcgcaaa aaaggggctg
     7321 tagtcgcggt tgctgctgga aatgataatg tttgggaaca ctataatgct cctgcagtgt
     7381 gcaatggaac gatcaaggtt gcttccaccg gaccagaagg cttgcgttcg tacttttcaa
     7441 attatgggcc cagtataacc ctcgcagcac cgggaggtga taagcgttat ggcacttcgg
     7501 gaggtatttt atctaccgta aacccgggag gtggatacaa ttcatcaggc tttgattttt
     7561 atcaagggac gagtatggct tcgcctcatg tagccggggt agcaggttta atttttgctg
     7621 tcagtaataa ggctttgaaa cctgaacaag ttgaacaaat tttatatgtt actacgcatg
     7681 agtttgggca aagcaacgac accaataaat cttgtgtcgg caaaaaacct tgtggacatg
     7741 gaatattgga tgcggataat gctgtgcagg cagcattaaa tggttatgat gtcttattct
     7801 ctgcgcctaa aatgattgct ctggcaatgc atgaatgtgg cgagaataga ttaaaacctg
     7861 gtaaaaatat cattaatgac agtactgcga cttggattca aattaaatca agctgtgaaa
     7921 ctggtgtaag ctatcagaag ccacgtattg aaaaagccaa ggatgggaaa atttatgctt
     7981 attatggttc cgtaagctat gaattggatc aatcagctta taaagaatgt cgtgtcgttg
     8041 gatatgatgg tgtaggctgt tatttataat atcaaatgaa attcgattct cagttttaag
     8101 acgtttttga tgagggaagc attcagagcc aatttactgg ttacttatgg aatccttctc
     8161 tcatcaaatg aaaatccctg cattcgctca ggtattaaaa ttttaacaaa gacattagaa
     8221 aattactcaa gccaattatt taatatgtca acaatttttt tggcttgttc agtactcgaa
     8281 atattgaatt tggatttttg ccaatattga ttgttacgtt tctgataacg ccgtatagaa
     8341 tattttacag gaccgtaatc attttttgtg ttatcccaat cctggtattt aaacataata
     8401 gtggtccatg ctcctttgga taggatttgt ttatcgagtt ccctggttgt ttccactcca
     8461 ttttcgttaa aagcaattgt tatgtcatct atggtttcag ccatttgtca tacttcctca
     8521 attatttccg atggattgta atttgccgtc gacaaaagtc aaggaaaagg ctggttgata
     8581 tggacctggt ttataaatcc agatttgtgg ttttgtctct gtaggaacca cttggttaat
     8641 gtaagtgtta ttgaccaggt ttggactgcc gcaagcgcca taaactttag tggcaggatc
     8701 tccgatttga atgcttgcgt tgccgcacaa ggaaaaagcg ttggtattgc cgccattcac
     8761 tcgaatatcc tggactttat tattgagaat atcgacttcc aattgcacgc cactactacc
     8821 tgtaggtata ttccatacgc cataaaaagc ggtaccttcc ggtacattta atgatcctga
     8881 atacaggcca tcagagtttc ccagattatt ataaattagt tgctgcacag ggattttctg
     8941 caaaataggc tgattggact cttgtttact cagtggttgt ccgcacgcag caatgacctg
     9001 atccatggac ataccaatat tgatataacc atgattctga gggcaataca aagattgtgc
     9061 gctcattaaa cggaatggca taattagcaa taataaactc ataagtaata tcgatttcat
     9121 agctttttcc attaatccgt atcaactatt agtatagttc tgtttgttta tttttgcttt
     9181 tataataact tccagggcaa agaagcgctt agaaacaggg taataatttt taatatcacc
     9241 atgataataa agggtgagaa atcaaaacct gaaatgtccg ggactataat tctgcctagt
     9301 cgtaaaattg gtgttgttat tatgcgcaag aaatcaccaa gcggatgttg ccatttggga
     9361 ttaacataac tcattattac tcgaatcaga acagcaaaaa ataatatttc gcaaggttga
     9421 attattaaat caacaagtat gtagagaaaa ataaagagga atggaatgat tgcatgatac
     9481 atcagtaaac tcagtgaaat tattttaaaa aattccacca ggattaaaac cccaaatgct
     9541 atccagtcgt atttttgtcc tggttgcaat ggctttttta taatccaata caagggtttc
     9601 actagaggat cagtaatggt atggattaat tgagagaatg gattaatagc actgatacga
     9661 aaaaatcgta tagcaattct aagccacaaa ctgaaaataa cgatagtaaa taataaggat
     9721 aataaaaaat acccgacagc agttaaacca gccattgttt tacctcttta gattgtattc
     9781 atcaataaag ccagcaaatt tattcatatg atttagaaat gaattgctta agcagtttat
     9841 taaccttcta tcataacgcg agcaaacctt gttccacaat gattgtttgc ttgaatagtt
     9901 caagaattaa
//
LOCUS       DATNSN010000126         9766 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.126, whole genome shotgun sequence.
ACCESSION   DATNSN010000126 DATNSN010000000
VERSION     DATNSN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.126"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            222..1829
                     /gene="murJ"
                     /locus_tag="VAH76_002382"
     CDS             222..1829
                     /gene="murJ"
                     /locus_tag="VAH76_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEO1386983.1"
                     /translation="MSSDKTLNVKATGIIALAVMCSRVLGLIREVLFNALFGSASMGI
                     FLIAFRAPNLLRDLFAEGALSVSFITVFSKKIETEGEKSAWQLASKMLTLTSVFMSIL
                     CLFGIIFAKYLIFILAPGFSVKDAETTIFLTQLMFPFILLVSLAAIVMGMLNSKNVFG
                     MPALASSFFNIGSILGGALCGWFIDPSFGERALIGLAIGTVIGGLLQLGVQFPSLRKV
                     GFYFKPNFHWYDSGVRNTLILTIPAVIAASAVQINVLINSGFASYLGKEAVTWLNSAF
                     RLMQFPLGVLGVAIATITLPVVSRLAATNNHSQFGSTLARAIRLAVFLTMPAAVGLWF
                     FANPIISLIYEHGKFHASDSLQTAYALQFYALGLVAYSCIKVLSPAFYAIDKKWTPMI
                     VSFATIILNIILNYFLIFKLSMGHRGLALSTTVSATVNFLVLYVLMTRLHNLQNRYLF
                     NTILRCVLASAILGFMCQSIFAYGADFLYHPSFWIRAVSLAISISFAVLVYLSMCVIL
                     RVDEAKSIFMIIRHRLGFPIKFKSKQV"
     gene            2145..3950
                     /locus_tag="VAH76_002383"
     CDS             2145..3950
                     /locus_tag="VAH76_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386984.1"
                     /translation="MGIFLETKRLILKRVELSDLDLLVALRSDLEVMENTGYGGTQTI
                     EEVSEYLDFAIPYQEKHGMGFCLVFEKESGNFVGEAGLFHLLFDDKQSEIELGYHLHK
                     KFWGKGYGTELAKALIQWGFQHLSVNKLISTTYPDNIASQKVLNKAGFDCMSKKQLPD
                     GEELFWYEIYKNDSVELVPYDSQWPKMAEVEIKKLRKILPTNHIIDIQHVGSTAIPGM
                     LAKPIIDIQITVDSLTAIKQTAIDVLKAYDYVYWAEDPDPEKMFFVKGMPPFGEKRTH
                     HVHIIEPKSKRGHDRIIFRDYLIAHPEVAREYEQLKIELAHEYTYDRERYTDAKTQFI
                     ASVLGKALVTDQIQNPTKYKGINELLHQLLLQIQSILANEFVGMYIGGSLASDSFNSE
                     TSDIDCYIITSKMLSENMIGKIEEMHNQFYSSKLKYAKKIEASYVPQKDLLDFDPQGM
                     RPYFNEGRYYLGHYGNNYLIELFVLREKGISIAGPDIKCLIQEISTQSLKAAIQKNLH
                     EYWESNLIDFSKFKRSDYQVFTILTMCRTLYSLETGKITSKIEAAQWAMQQLDTKWKK
                     LIELAAAWEPSQEINRLEETQNFVRYVLNKSREYQ"
     gene            4063..4668
                     /locus_tag="VAH76_002384"
     CDS             4063..4668
                     /locus_tag="VAH76_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1386985.1"
                     /translation="MSKLNKTRVYEVYDEIINWFDDARTKNLMESEYLNLIVNAIPPK
                     SSILDLGCGTGEPIAQFFIEKGFKLTGVDGSQKMIELCRKRFPDERWIVSDMRDINLQ
                     QQFDVILAWHSFFHLDHDSQRNMFKIFESHTKPGGVLAFTSGEEEGEVWSDNGGQQLY
                     HASLSTKEYESLLKNSSFKVLVHKVCDPECGQATVWVAQKI"
     gene            4709..5203
                     /locus_tag="VAH76_002385"
     CDS             4709..5203
                     /locus_tag="VAH76_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1386986.1"
                     /translation="MNPHFKIRFAILSDVPLILQFIKELAEYEQLLHEVVATEDILQE
                     TLFGPKSHAEVVIGYLEEKPVSFALFFHNFSTFLGRPGIYLEDLYVRPEARGQGIGKM
                     MLSYLAALAKNRKCGRLEWWVLDWNETAINFYKSIGAKPMDEWTVYRVTGQALDNLAN
                     GFTA"
     gene            5847..6233
                     /locus_tag="VAH76_002386"
     CDS             5847..6233
                     /locus_tag="VAH76_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1386987.1"
                     /translation="MIRKLFIILLSMSLTACSVVMAAKGNGVNPKELGNCKTRSCLIA
                     AGAIPIEHTKGKHGKRSSENFRATMPTGSAARAAMHGLLDVGTLGLWEVAGTPIEAVK
                     GKKTGYVVAVKYDEDGSTIKHMSFHF"
     gene            complement(6344..8251)
                     /locus_tag="VAH76_002387"
     CDS             complement(6344..8251)
                     /locus_tag="VAH76_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO1386988.1"
                     /translation="MKEEKRSKLVSMTIRNIGCIGNNGLEIKLDKIVCLVGKNNSGKS
                     TILRAYELAKGSVQFNIEHDRYQLASIEQPSEIILEVHIPKNIGNVDERWKTEHDGLL
                     IVKSIWRWEAPDFQKVRKTWDPQSQNWADDGKAGGADPVFSSRLPRPLRIGSLEDADK
                     TQEILLNLALTPLVDLLKKEQTNNESEFAKALINVTKQINALSTSHQEHFNKIAERIT
                     SGFQNVFPNLHVRLNIATAPLTPNVPDLIKKGSGLRINDGNADTSLNQQGTGTRRALF
                     WAMLQVHNELTREKESREKQIKELTTKLTKTKKKAPIDSKEINELQERITSLENNDPL
                     PNEIDDPALPGYLLLIDEPENALHPMAARAAQQHLYSLAEDPDWQVMITTHSPYFINP
                     FEDHTTIVRLDRPNQESSWEPKIYRSDTITFEEDEKQRLQALQHIDPSFAEIFFGSYP
                     ILVEGDTEHAAFIAAILEKKHELMERVTIIRARGKAILVPLIKVLKHFKIDFGILHDC
                     DSPYTKSGNKNGMWTENGKIRSSIISARDTGLTIRHRVSFPDFERFLGYDEESKDKPL
                     NAYRNITTDTSLCNRVQELLEELINSDQYEPFSNANLTDQEDYNKCLLEKITQWAKDN
                     GNENDIRYKGK"
     gene            complement(8273..9553)
                     /locus_tag="VAH76_002388"
     CDS             complement(8273..9553)
                     /locus_tag="VAH76_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO1386989.1"
                     /translation="MSEPTIICPACKSEIKLTESLAAPLIESTKKQYQEQLEKKDLEI
                     AGREEAIRQKENNLKEAQRHMDEQVLDKVQEQLKKERAAIIQEEAKKAKLALGTELEN
                     RTKELSDLQELLKAREEKLAIAQKAEAEFIRRQRELDDAKREMDLTIEKRIREELENA
                     RVNAKKEAEDSLNLKLKEREQTISSMQKQIEELKRKAEQGSQQLQGEVQELALESMLQ
                     TKFPLDSIEPVPKGEFGGDVIQKVAGSNGQICGTILWESKRTKNWSEGWLTKLKEDQR
                     TAKAEISVIVSQALPSGVETFEMINGVWVTHPRAAYPVAMVLRQSLLEIAMARNSAEG
                     QQTKMEMVYQYLTGPRFRQRVEAIVDAFSSMQSDLEKERKVIMKQWAKRNEQIERVMG
                     ATIGMYGDLQGIAGKSLQEIDGLELKSLGSDDEL"
BASE COUNT         2842 a         1734 c         1830 g         3360 t
ORIGIN      
        1 tgctattaag tccaactcct cttgggttat taagatattt tggtaccaat tttggtacca
       61 gttttgaaat gtaattgatt acggtaattg tttccaattt aatacctact tcaatgagtt
      121 aacaccacaa tatctattgc atatgccaag catgataggg tatcgctcct cttatacttg
      181 ggctatcata ttaatgtttt tgaagagact ttggataatc catgagtagt gataagacac
      241 taaatgtaaa agcaacaggg atcattgccc ttgctgttat gtgtagtcga gtattaggtc
      301 ttattcgaga agtcttattc aatgctttat ttggcagtgc ttccatgggg atttttctta
      361 tcgcattccg tgcgccaaac cttttgaggg atttgtttgc agagggcgct ctctctgttt
      421 cttttattac tgttttctct aaaaaaattg aaactgaagg agaaaaatca gcttggcagc
      481 tggcttcaaa aatgcttaca ttgaccagtg ttttcatgag catcttatgt ctctttggga
      541 ttatttttgc caaatacctc atttttattt tagcgcctgg tttttcagtg aaggatgctg
      601 aaacaacgat tttcctcacc caattgatgt ttcctttcat tttattagtg tctcttgctg
      661 ccatagtaat ggggatgctg aattctaaaa atgtttttgg tatgcctgcg ttagcctcga
      721 gtttttttaa cattggctct attcttggtg gcgctttatg tggatggttt attgatccat
      781 catttggaga gagggcactt attggtttgg caatcggaac ggtaataggg ggacttttac
      841 aactcggagt acaatttccc agcttaagaa aggttggttt ttattttaaa cccaattttc
      901 attggtatga ttctggggtt cgaaacactt tgattttaac tattccagcg gtgatcgccg
      961 caagtgcagt gcaaataaat gtacttatca attcaggatt tgcctcatac cttggaaaag
     1021 aagcagtaac atggctcaac agcgcattta gacttatgca atttccactt ggagtgttag
     1081 gtgttgctat tgctacgatt acattaccag tcgtatctcg tcttgctgca acgaacaatc
     1141 actctcaatt tggatcaaca ctggctcgcg cgatccgtct agcggtgttt ttgacaatgc
     1201 cagctgcagt tggactttgg ttttttgcaa accccattat aagcctcatt tatgaacacg
     1261 gaaaatttca tgcgtcggat tccttgcaaa ccgcatatgc attgcaattt tacgctcttg
     1321 gacttgtggc ttactcatgc atcaaggtgc tttcaccagc cttttacgcc attgataaaa
     1381 aatggactcc catgattgtc agttttgcaa ccattatctt aaatattatt ttgaattatt
     1441 ttttaatttt caaattgagt atggggcatc gtggactcgc tttgtccaca acagtttctg
     1501 ctactgttaa ttttttggtt ctatatgttc tcatgacccg cttgcataat ttgcagaata
     1561 gatacctttt caacactata ttacggtgtg ttttagcatc tgcaatatta ggttttatgt
     1621 gtcaatctat cttcgcgtat ggagctgact tcctttacca tccttcattt tggatacgtg
     1681 cagtttccct tgctatttca atttctttcg ctgtcttagt gtatctgagc atgtgtgtaa
     1741 tccttcgagt ggatgaggca aagagtattt ttatgatcat tcgtcatcga ttaggattcc
     1801 ctattaagtt caaatcaaaa caggtataaa cattaataat tgaataatag agaagttatt
     1861 gagagactac agtagtctat gagaaagttt gctgttatca caaagtgact ttggtttatc
     1921 aaaccattta ataagtgtat cttcccctag attatcatat atataggata aacaggtcgt
     1981 tcaggatccg accctgatcc tgttttccag aagttatccc tgatttaaaa ttaaatataa
     2041 tattttcgtg ctacccctgt gctacctttt gtagcaaatg ctatcgtaga agcatcaaat
     2101 aaaaagtata attccttcca gtttaaaata tagatagaca cccaatgggc atttttctag
     2161 aaactaaaag actaatactt aaaagagttg aattatccga tcttgatctg ctagtagcat
     2221 tgagatctga tttagaagta atggaaaata ctggttatgg gggtactcag acaatagaag
     2281 aggtaagtga gtacttagat tttgctattc cttatcaaga aaagcatgga atgggatttt
     2341 gtctggtatt tgaaaaagaa agcggaaact ttgttggcga agcgggttta ttccatttgt
     2401 tatttgatga taagcaatcc gaaattgaac ttggttacca cttgcataaa aagttttggg
     2461 ggaaaggcta tggcactgaa ttagcaaagg cactaattca gtgggggttt cagcacttat
     2521 ctgtcaacaa gctcatttct accacctatc cagataatat cgcttctcaa aaagtcttaa
     2581 acaaagcggg ctttgattgc atgagtaaaa aacaattgcc tgatggcgag gaattgtttt
     2641 ggtatgaaat ttacaagaat gattccgttg aattagtgcc ttatgattct caatggccaa
     2701 agatggctga agtggaaatt aaaaagctac gaaaaatcct gccaactaat catattattg
     2761 atatccaaca tgttggtagc acagctattc caggaatgct ggctaagcct attattgata
     2821 ttcaaattac tgttgattct ctaaccgcta taaaacagac agcgatagac gtattaaaag
     2881 cctatgacta cgtatattgg gccgaagatc ctgatcctga aaaaatgttt tttgtaaaag
     2941 ggatgccacc atttggtgaa aaacgaacac accatgtgca tattattgag cctaaatcaa
     3001 agcgtgggca cgatagaatc atttttaggg attatttgat tgctcatcct gaggtagcgc
     3061 gtgagtatga acaattaaaa atagaattgg cacatgaata tacttatgat cgggagcggt
     3121 ataccgatgc taaaacacaa ttcattgcta gtgtattagg caaagctcta gtaactgatc
     3181 aaattcagaa tccgacaaaa tataaaggaa taaatgaact actacaccag ctcctcttac
     3241 aaatacaatc tattctagca aatgaatttg taggtatgta cattggaggt tctctcgcaa
     3301 gtgattcatt caatagcgaa acaagtgata ttgattgtta cattattaca agcaagatgc
     3361 tttctgagaa tatgatcgga aaaattgagg aaatgcacaa tcaattttat tcaagtaaat
     3421 taaaatatgc gaaaaaaatt gaagcttcct acgttcctca aaaagatctc ttagattttg
     3481 atccccaagg catgcgccct tattttaatg aaggtcgcta ttacctgggg cattatggca
     3541 ataattattt aatcgaacta tttgtactaa gagaaaaagg aatttcaata gcagggcccg
     3601 atataaaatg tttaatccaa gaaatatcca ctcaaagttt gaaggctgct atacagaaaa
     3661 atttgcacga atattgggag tctaacttaa ttgatttttc aaaatttaaa cgcagtgact
     3721 atcaggtgtt tacaatttta accatgtgcc gcacccttta ttctttagaa acaggcaaaa
     3781 taacttctaa aatagaagca gcacaatggg caatgcagca gcttgatact aaatggaaaa
     3841 agttgattga attagctgcc gcttgggagc caagccagga aataaataga ttagaggaaa
     3901 cgcagaattt tgtcaggtat gtgctgaata aaagtcgcga gtatcaatga gcagatcaca
     3961 ttacttttca gaataaatga agactgaaaa ttttttgtag tagtctgata aaatctcacc
     4021 catcaataaa tccagctata gttattttag gagcaattga gcatgagcaa gttaaataaa
     4081 actagagtct atgaagtata tgatgaaatc attaattggt ttgatgatgc tcgcactaaa
     4141 aacctcatgg aatcggaata tcttaattta attgtgaatg caattcctcc gaagagctca
     4201 attttggact tgggctgtgg aactggagag cctatcgcgc aattttttat tgaaaaaggc
     4261 tttaaactta ctggtgttga tggcagtcaa aaaatgattg agctttgtag gaagcgattt
     4321 ccagatgaac gctggatagt ctcagatatg cgcgatataa atttacaaca acaattcgat
     4381 gtaatacttg catggcatag cttttttcat ctggatcatg acagccaacg taatatgttc
     4441 aaaatatttg aatcccatac caaacctgga ggagtactag catttacttc tggagaagaa
     4501 gaaggcgaag tctggagtga taatggaggc caacaacttt atcatgcatc tttgtcgaca
     4561 aaagaatatg aaagccttct caaaaattca tcatttaaag tgctggtaca taaagtctgt
     4621 gatcctgagt gtggccaggc tacagtttgg gtagcgcaaa agatttaaaa tccttcgtta
     4681 taaaccaaaa ttatcagtgg aattacctat gaaccctcat tttaaaataa gatttgcaat
     4741 actgtctgat gttcctttaa ttttgcaatt tattaaagag ttagcagaat acgagcaatt
     4801 gctccatgaa gtagtagcaa ctgaagacat tctgcaagaa actttatttg gacctaaatc
     4861 tcacgcagaa gtagttattg gatatttgga agaaaagcct gttagttttg ctctattttt
     4921 tcataatttc tctacttttt taggtaggcc aggtatctat ttagaggatc tctatgtgag
     4981 gccagaagct cgcggacaag gaattggaaa aatgatgtta tcttatttgg cagcgcttgc
     5041 taaaaataga aaatgtggaa gattagagtg gtgggtatta gactggaacg agactgccat
     5101 taatttttat aaaagcattg gcgccaaacc catggacgag tggacagtat atcgagtgac
     5161 tgggcaggct ttagataatt tagcaaatgg ctttactgca tgacaattaa taatatatag
     5221 ccacaatttt tagtaatttc atctcctaac gcccatcatg atcgcatttg gaatcctatt
     5281 caaatatttt tctatcaaat cttggacttt gtttatagaa acgctgggca ataacgcggg
     5341 tattcctgag ctaagccatg ggaacgtttt tccaaaagct ttatgtcata ttcaatcatg
     5401 tcctctatat caagtttatt gctgtacatt tcttggttat gaatcccatg gcccccatca
     5461 taaagcgcta acaagctaaa aggtcagatc tcgctaaagg ttcagatctt gccttttgcc
     5521 tatcaaagta gttttgggga attttagaga aagcaaaaag caagatctga acctgaccta
     5581 catgaccctg acctacaaat agtattactt gtaatcaata ggttcgcagt tcgaccccgc
     5641 gcaacggtat tataaaaccc attggttacg ttggatcatg taatcacatt gctgtagcaa
     5701 acgaaattta acaacaattt agtaaatcat acgatttttg aagttcctgt ttataagcaa
     5761 acagactgct ctagctatga aaatattttg atcaatgtta tcatctgatc attttttaac
     5821 ctttggtaga tgaaatagtg cttataatga ttcgaaaatt atttatcatc cttcttagta
     5881 tgtcgctaac tgcatgttct gtggttatgg cagcaaaagg aaatggtgtt aatccgaaag
     5941 aattaggaaa ctgcaaaact cgttcgtgtt tgattgctgc tggagctata ccaattgagc
     6001 ataccaaagg taagcacggt aaacgctctt ctgagaattt tcgtgcaact atgcccaccg
     6061 gatcggctgc tcgtgcagca atgcatggtc ttcttgatgt aggtacatta ggtctttggg
     6121 aagttgcagg tacgccaatc gaggctgtta aaggtaaaaa aacaggctat gtagttgctg
     6181 ttaaatacga tgaggatggt agtactatta aacacatgtc ttttcatttc tgatagcagc
     6241 taaaggggca gatcttgcct tttgcctatc aatgggggaa ttttagagaa agcaagatct
     6301 gaccctgacc tacgcagata agttttgcaa ttaattaatc caattatttg cctttataac
     6361 gaatatcgtt ttcatttcca ttatcttttg cccattgagt tattttttca agaaggcatt
     6421 tattatagtc ttcttggtca gtcagatttg catttgaaaa tggttcatat tgatcactat
     6481 ttattagttc ttcaagtaac tcttgtacac gattacacaa gcttgtatct gtggtaatat
     6541 ttctatatgc atttaagggt ttgtcttttg actcctcgtc atatcctaaa aatctttcaa
     6601 aatctgggaa acttactcta tgtcgtattg taagaccggt atcacgtgca gatattatag
     6661 aacttcgaat ttttccattt tcggtccaca ttccattttt atttcccgat tttgtataag
     6721 gtgaatcaca atcatggaga ataccaaaat caattttgaa atgtttcagt actttaatta
     6781 aaggaaccag aatagccttc cctcgggctc gtattattgt gactctttcc attagctcat
     6841 gttttttctc taatattgca gcaataaatg cggcatgttc tgtatcgcct tccactagta
     6901 tggggtatga gccaaagaaa atttcggcaa aactgggatc aatgtgctgt aatgcttgca
     6961 gtcgttgttt ttcatcttcc tcaaaagtaa tcgtgtcgga gcgataaatt ttgggttccc
     7021 acgatgattc ttgatttggc ctatcaagcc ggacaatagt tgtatgatcc tcaaaggggt
     7081 taatgaaata cggtgaatgt gtagttatca ttacttgcca atcaggatct tcagcaaggc
     7141 tatataaatg ttgttgtgca gctcgtgctg ccattggatg taatgcattt tccggttcat
     7201 caatcaataa aagataacct ggtaatgcag gatcgtctat ctcatttggt agcggatcat
     7261 tgttttcaag agaagttatt cgttcttgta attcatttat ttctttagaa tcaattggtg
     7321 cttttttctt tgtctttgta agcttggtag ttaattcctt aatctgtttc tcacgagact
     7381 ctttctcacg tgttaattca ttatgtactt gtagcatagc ccaaaacagt gctcgacgtg
     7441 ttcctgtacc ttgttgattt aatgatgtat ctgcattgcc gtcatttata cgtaatccag
     7501 agcctttttt aattaaatca ggaacatttg gagttaaggg ggcagttgca atgtttaatc
     7561 tcacatgtag atttgggaaa acattttgaa atccactagt aatccgctcc gcaattttat
     7621 tgaaatgctc ctgatgtgaa gtacttaaag catttatttg ttttgttaca tttattaaag
     7681 cttttgcaaa ctctgactca ttattggttt gttctttttt gagtaaatca acgagtggag
     7741 ttagagcaag attgagtaga atttcttgtg ttttatcagc atcttcaagt gatccaatac
     7801 ggagaggtct tggtagtcga gaactaaaaa ctgggtcggc tcctccagct ttaccatcat
     7861 cggcccaatt ttgagattgt ggatcccaag tttttctgac tttttggaaa tctggtgctt
     7921 cccatctcca tatactttta acaattagta acccatcgtg ttctgttttc cagcgctcat
     7981 caacattccc gatatttttg ggaatatgaa cttcaagaat aatttctgat ggttgttcta
     8041 tggatgctaa ttgataacga tcatgttcta tattaaattg aactgagcct ttagctaatt
     8101 cataagcacg aagtatggtt gattttcccg aattattttt tcctactaag cagacaattt
     8161 tatctaattt tatttcaagt ccattatttc caatgcatcc aatattacga attgtcatgg
     8221 atactaattt tgagcgtttt tcttctttca tttgttatct taaagctgat aattaaagtt
     8281 catcgtcaga cccaagtgat tttaattcca atccatcaat ttcttgtaag gatttgcctg
     8341 caatgccttg taaatcacca tacataccga tcgtggcgcc cattacacgt tctatttgct
     8401 cattgcgttt ggcccattgc ttcataatga cctttcgttc tttctccaaa tctgattgca
     8461 tagatgagaa agcgtcaacg attgcttcta ctcgttgtcg aaatcgaggg ccagttaaat
     8521 attgataaac catttccatt tttgtttgct ggccctctgc ggagttgcgt gccatcgcaa
     8581 tttcaagaag tgattgacga agtaccatag caacaggata agccgctctt gggtgggtga
     8641 cccaaactcc attaatcatt tcaaaagttt ctacaccaga aggcaaggct tgactaacta
     8701 taacggaaat ttctgcttta gcagttcgtt gatcctcttt caattttgtt agccatcctt
     8761 cgctccaatt tttagttctc ttggattccc ataaaatagt accgcatatc tggccgtttg
     8821 aacctgcaac tttttgaatt acatctccac caaactctcc tttaggaact ggttctattg
     8881 aatcaagcgg aaatttggtt tgcaacattg actcaagtgc caattcttgt acttcgcctt
     8941 gaagctgttg agaaccttgt tcagctttcc tttttaattc ttcaatttgt ttttgcatgg
     9001 aagaaatagt ttgctctcgt tctttaagtt ttaaattaag tgaatcctcc gcttcttttt
     9061 ttgcattcac acgagcattt tctaattctt cacgaatacg tttttctatt gttaaatcca
     9121 tttctctttt agcatcgtcc agttcacgtt gtcttctaat gaattcagct tcagcttttt
     9181 gagcaatagc taatttttct tcacgagctt ttaaaagctc ttgtaaatct gataattctt
     9241 tagttctgtt ttcaagttca gtccctaatg caagctttgc ttttttagct tcttcttgaa
     9301 taattgcagc acgttctttt tttagttgct cttggacttt atctaaaact tgttcatcca
     9361 tgtggcgttg agcttctttt aaattgtttt ctttttgccg gattgcctct tcacgaccag
     9421 cgatttcaag atcttttttc tctaattgct cttgatattg ctttttcgtt gattctatca
     9481 aaggagcggc aagggactca gttaatttga tttcagattt acaggcaggg cagataatag
     9541 ttggttcaga cattgttata ttatattctc tgttgttttg atctaaatta attttagcaa
     9601 ataatattta gttttgattt tagatttttt gaaatttcta tatatctaat ttaggaaaat
     9661 tttctgaaaa ctccgattaa atttctctac ccctataatt gtttcctatg tttggtagat
     9721 ataaattgat gtggagacga tgatagccta gtaatgatct aagtac
//
LOCUS       DATNSN010000127         9450 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.127, whole genome shotgun sequence.
ACCESSION   DATNSN010000127 DATNSN010000000
VERSION     DATNSN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.127"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..794)
                     /gene="motD"
                     /locus_tag="VAH76_002389"
     CDS             complement(<1..794)
                     /gene="motD"
                     /locus_tag="VAH76_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEO1386990.1"
                     /translation="MGNTSKMKSKQKKTEEHQDNHRWVVSYADFITLLFAFFVVMYAI
                     SSVNVSKYKSLSEGMKSAFNQKDQNKATQSTDNLKDGPEEKKTKGTFKDGLDELNKSL
                     SELEDGDFKLNRQEGWVEIDIKAGSLFDSGSADIKPEALIKLMKLAEKIKNLPFTVVV
                     EGYTDNVPIETPQYPSNWELSAARAATVGRILNGFGIANHRILVTGYGDQYPITDNYT
                     EAGRSQNRRVNILIAKNRKVDRILNPDLSRVHFSVISNTNSVEFNK"
     gene            complement(751..1524)
                     /locus_tag="VAH76_002390"
     CDS             complement(751..1524)
                     /locus_tag="VAH76_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEO1386991.1"
                     /translation="MDTLTLIGLLTGFLAIIVGQIFEGGDLNSLLNLPALLIVMGGTL
                     GAVMVQTPLTTFKRAFRILPWVVKPPHHNFEECRTTLIELSRKARQLGLLSLEDRLDV
                     ETNPLIYKGLELLVTGVDKLTLRQVLEAEIDRTEHQDLRAAHVFESMGGYSPTIGILG
                     AVLGLIQVMRNLADPSQLGLGIAVAFVATIYGVGFANLFFLPVANKLKSCISHRVHFD
                     EMVVEGLVAMASGESPNMLQLKLNNYGQHKQDEIKTKEN"
     gene            complement(1534..2250)
                     /locus_tag="VAH76_002391"
     CDS             complement(1534..2250)
                     /locus_tag="VAH76_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEO1386992.1"
                     /translation="MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLD
                     DLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMI
                     SDAVRILEHKLGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFD
                     DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSE
                     SRISQILSQATHRIRSRLPE"
     gene            complement(2347..3216)
                     /locus_tag="VAH76_002392"
     CDS             complement(2347..3216)
                     /locus_tag="VAH76_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEO1386993.1"
                     /translation="MSKLSKGIKDQASGLRNLSRNKPVKVIAVSAGKGGVGKSNISVN
                     LAIALSQLNYSVMLLDADLGLANVDIMLGLHTKYNLSHVLQGICHLSDIILHGPAGVR
                     VIPAASGTEFMTQLSPAEHAGIIDSFNELTDDLDYMIIDTAAGISETVLSFTRSSQEL
                     IVVVCDEPTSLTDAYALIKVMSKRYEWTHFHILANMVRNVKDGRDLFNKLFRVSGQFL
                     DVQLDYLGAIPFDENIHKSVKKQNPILIAYPDSPAAHALRELAESVSNWSFKPSLGGN
                     TSFFLERLVAGQF"
     gene            complement(3203..4342)
                     /gene="flhF"
                     /locus_tag="VAH76_002393"
     CDS             complement(3203..4342)
                     /gene="flhF"
                     /locus_tag="VAH76_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEO1386994.1"
                     /translation="MKLKRFVAADTRSAMQQIKAAFGPDAVILSSHNIKDGVEIVAAI
                     DFDETVISTQAAVAIAEPPSRTEKYSAQTPLDEMRQEILTLRGMLEAQLRGNVGIHEP
                     LHTVMLQKLLYLGVSPNTATIWARSVKPDLNQQRAWQFILSQVTEQLPIRDSSRIEEG
                     GIYAFVGPTGVGKTTTLAKIAARFALRFGADKLGLVTMDTYRIAAQEQLILYGKILGV
                     KVCIAQDEVSLARVLRQLNDKKLILIDTAGMNPSDERVTKQMTVLSNYLQSIEKVLVL
                     PATSHYQVLIDAIKRYQANHVDQCIITKLDESLAVGGAISAVIESNLALGYLTDGQRV
                     PEDIKIATRHQLVELFAQQELQLFQEERDISKIHPNVRRENYVEA"
     gene            complement(4383..6461)
                     /gene="flhA"
                     /locus_tag="VAH76_002394"
     CDS             complement(4383..6461)
                     /gene="flhA"
                     /locus_tag="VAH76_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEO1386995.1"
                     /translation="MNNVMNYIEYWMRQGFGTPLILIVMISMMMLPLPPFLLDIFFTF
                     NIALSLVVLLAVIYSERPLDFAVFPTVILVATLLRLALNVASTRVVLLNGHNGTDAAG
                     QVIKSFGEVVIGGNYTVGIVVFIILVVINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNAGLINQEQAIKRRTEVAQEADFYGSMDGASKFVRGDAIAGILVLIINIVGGL
                     IIGIFQYNMNFNDAVHNYILLTVGDGLVAQVPSLLLSTAAAIIVTRVSSAQNMGQAVI
                     SQVFAKPRSLVIAAAIIGLIGIIPGMPHIAFILLSAGLAGIAYLFINQEKVKREQQDK
                     EQNEFSQTSDSKLSELSWDDVNPVDVIGLEVGYRLIPMVDNAQGGILLSRIKGVRKKI
                     SQELGFLIPSVHVRDNLDLKPNHYRLSLAGVIMGESMIHPDKWLAINPGQVFGNLEGI
                     PTRDPAFGMDAVWINENQKEKAHTLGYTVVDASTVVATHLSMILEQNSQQLLGYEETQ
                     QLLDKLAISSPKLVKELVPDGLTLGMVSKVLQGLLSEHIPLTDIRTIVETLAEYASKS
                     KDTEYLISQVRIALSRLITQKISGLNEELPIITLKPELEQLLHNTLQNSGHGGVSFEP
                     GMADKIQQALIQFAAQQQAKQESSVLVVQPSIRTVLARFVRTISNDFHVLSYQEIPDN
                     KQIRIIGTVG"
     gene            complement(6458..7606)
                     /gene="flhB"
                     /locus_tag="VAH76_002395"
     CDS             complement(6458..7606)
                     /gene="flhB"
                     /locus_tag="VAH76_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEO1386996.1"
                     /translation="MADEEQSQEKTEQPSSKRLKESRKKGQVARSKDFNATVILLFTG
                     LGFFIFGKQLSLQIASIMQQAFDFDRDILVTGNNSLENLFQLTKSGLWSVVPLLLVIF
                     ILSLLAPLLMGGWVFSGQSIQPKFSRLNVLKGLKRMISLKGFVEMLKAFLKFVLVASA
                     SILVLRSQVPLLLELGKAPLEIAITSGVMILLKSFVLISASLILIAAIDVPFQLYEHS
                     KSIKMTKQELKDEYKETEGKPEVKSAIRRAQQETARRRMMSEVPKADVILTNPTHYAV
                     ALSYQKKGKKAPVVIAKGKNLVAFQISKVAKEHKIPIISVPALARAIYFSTKLNAEIP
                     RGLYVAVAQVLAYIFQLRDRQRYDYKPEILQNVPIPPELAREAEEEIE"
     gene            complement(7606..8376)
                     /gene="fliR"
                     /locus_tag="VAH76_002396"
     CDS             complement(7606..8376)
                     /gene="fliR"
                     /locus_tag="VAH76_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEO1386997.1"
                     /translation="MNLDYQTMIRLMSQFIWPMVRIGGLMLTVPVLSSALLPARIKIL
                     FVFTLSCVCFTYVPHNLSFENFNGLYLVYLVQELLLGLLMGFVLQIVFQVFILGGQII
                     SMQAGLGFAVMVDPTSNASVPLISQLYLMVTTLIFLALNGHLAILETLIESFRIMPIG
                     KSSLDPSVVWSTIMFSGWMFKEAVLISIPAILSLLIVSLSFGVMARVAPQLNLFSLGF
                     PITLLMGFVIIKIGLPTVGTEMVESIKHGIQFITGILR"
     gene            complement(8380..8649)
                     /gene="fliQ"
                     /locus_tag="VAH76_002397"
     CDS             complement(8380..8649)
                     /gene="fliQ"
                     /locus_tag="VAH76_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEO1386998.1"
                     /translation="MTPESVVSLFSDSVYIILLLLAVLILPGLIVGLIISMFQAATQI
                     NETSLSFIPKLFVTFLVLILAGPWLLKTLINYTDTLISNIPYLIG"
     gene            complement(8658..9407)
                     /gene="fliP"
                     /locus_tag="VAH76_002398"
     CDS             complement(8658..9407)
                     /gene="fliP"
                     /locus_tag="VAH76_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841869.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEO1386999.1"
                     /translation="MKIKSWLLILILFLLLPVAGHTDPLSFPVVTMQPGTDGSQSYSI
                     NLQILIFMTLLTVLPGLLMAMTSFTRIIIVLSILRQAIGMAQTPTNQILIGIALFMTY
                     FVMSPVFNKINETAIQPYMNERIDFSLALSNAQKPLRQFMLNQTRKNDLSLFEKFAHN
                     VPKTLDEIPMSVLIPAFITSELKTAFQIGFILFLPFLIIDLVVSSVLMAMGMMMLSPL
                     IISLPFKIMLFVMVDGWTLVLGSLASSFALA"
BASE COUNT         2994 a         2064 c         1591 g         2801 t
ORIGIN      
        1 tttttattaa actctactga atttgtatta cttataacac taaaatggac tctgcttaaa
       61 tcaggattga gaattctgtc cacttttcta tttttagcga taagaatatt cactcttcga
      121 ttctggcttc ttccagcttc agtgtaattg tccgttattg ggtattgatc accatatcct
      181 gtaaccaaaa tacgatgatt ggcaattcca aaaccattta aaatgcgacc aacagtagct
      241 gctcttgcag ctgataactc ccaatttgaa gggtattggg gcgtttcaat aggcacatta
      301 tcagtgtatc cctcaacaac cactgtaaaa ggtagattct taattttctc cgccagcttc
      361 attagtttaa tcaatgcttc tggtttaata tcagcactac cagaatcaaa taatgagcct
      421 gcttttatat ctatctcaac ccatccctct tgtctgttta atttgaaatc accatcttca
      481 agttcagata gtgatttatt cagttcatcc aaaccatctt taaaagttcc cttggtcttc
      541 ttttcttcag gaccatcttt aaggttatct gtcgattgag tcgctttatt ttgatccttt
      601 tgattaaatg ccgacttcat tccttcagaa agagatttat attttgaaac attaactgaa
      661 gaaatagcat acatcacaac aaaaaaagca aataataagg taatgaaatc agcatacgat
      721 acgacccatc gatgattatc ctgatgttct tcagttttct tttgttttga tttcatcttg
      781 cttgtgttgc ccatagttgt ttaactttaa ctgcagcata ttgggactct caccactcgc
      841 catggcgacc aggccttcaa cgaccatttc atcaaaatgc accctatgag aaatacaact
      901 ttttaattta ttagcaacag gtaaaaaaaa cagattcgcg aacccaacac cataaatagt
      961 tgcaacaaaa gcaaccgcaa tacctaaacc caattgactg ggatcagcga gattcctcat
     1021 gacttgaatt aaacccaata ccgcgcctaa aattcctatg gtggggctat acccgcccat
     1081 gctttcaaag acatgcgcgg cacgtaaatc ctgatgctcc gttcgatcta tttctgcttc
     1141 caaaacttgt cgtaaggtaa gcttgtccac tccggtgacc agtaattcta accccttata
     1201 aattaaagga ttggtttcga catccaaacg atcttctaag gaaagaaggc caagttggcg
     1261 agcttttcta gataactcta tgagagtcgt tctgcattct tcaaaattat gatgaggagg
     1321 tttcactacc cagggcaata ttctaaatgc ccttttaaaa gtggttaaag gagtttgcac
     1381 cataacagcc cctaatgttc cacccatgac aatcagtaag gccggaagat taagtaaaga
     1441 atttaaatct cccccctcaa atatttggcc tacgataatg gcgagaaaac ctgtcaacaa
     1501 tcctattaaa gtcaaagtat ccatacttat tttttattcc ggtaatcttg atctaatgcg
     1561 atgcgttgct tgacttagaa tctgtgaaat tcgtgactca ctaacctcca gtatttcccc
     1621 gatttccttc aaattcaaat cctgctcata atacaaagaa agcaccagcc tctctttacg
     1681 agggagacta tcaatcactt gggttaatcg attcatcata tcacttcgca taacattcct
     1741 gtgcggttca gttgagccat tcccttcatc atcaaataag atatcatcgg ttactcccaa
     1801 gtcatcaaaa ccatataaat ggctgctaat ggaatcttgc aacatatcat gataatcatt
     1861 taatgacaca cccaactctt cagcaatttc attatcctta gcctcacgcc caagtttatg
     1921 ctccaatatg cgcacagcat cagaaatcat tctggaattg cgataaacag aacgcggcac
     1981 ccaatcatta cgtcttactt catcaagcat atgtcccctg atacgtattc ctgcataagt
     2041 ttcaaaggat gcccccttgg atgaatcata atgtcttgct gcttcaagca gaccgagcat
     2101 ccctgattgt attaagtcat ccagttgtac actctgcggt aatcgcccga gcagatgatg
     2161 tgcaatgcgt tttaccaatg tggcatgtgt ttttaccaga gcttcctggg tttgttgatt
     2221 tactttgctg tatgcagcca aagcatccac gatactctcc ttacaataac agcagattgt
     2281 agttattagt aatttggtaa atctaaaaca aacagagtac agctaatttt aagtatagcg
     2341 aataatttaa aattgtcccg caaccaaacg ctccaaaaag aaactggtgt ttccacctaa
     2401 agacggttta aatgaccaat tactcaccga ttcagctaat tctctcaagg catgagcagc
     2461 aggtgagtcg ggataagcga ttaaaatagg attttgtttt tttactgatt tatggatgtt
     2521 ttcgtcaaaa ggaatcgcgc cgaggtaatc taactgcaca tccagaaact gtcctgagac
     2581 ccgaaacagt ttattaaaca ggtcacgacc atccttaaca tttctaacca tattagccag
     2641 aatgtggaaa tgcgtccact cataacgttt gctcatcact ttaattagtg cataagcatc
     2701 tgtcaaagaa gtaggttcat cacagacaac aacaatcaat tcttgcgatg aacgagtaaa
     2761 actcaataca gtctcggaaa tccctgctgc ggtatcaata atcatatagt ccaaatcatc
     2821 agttagttcg ttaaatgaat caatgattcc cgcatgttcc gcaggactca actgcgtcat
     2881 aaactctgtt ccggaagcag caggaataac cctgacgcca gcgggaccat gtaaaataat
     2941 atcacttaaa tgacatatgc cttgaagaac atgagataaa ttgtatttgg tatgcaaccc
     3001 cagcataata tccacatttg ccaaacctaa atcggcatcc agaagcataa cactataatt
     3061 tagttgagat aacgctatgg ctaaattgac cgagatatta cttttgccta caccgccttt
     3121 gcccgctgaa acagcaatca ctttgacagg tttgtttcgg gacaaatttc taagaccaga
     3181 agcctgatct tttatacctt tgctaagctt cgacataatt ctctctccta acattcggat
     3241 gtattttgct gatgtccctt tcctcctgaa ataactgaag ttcttgttgc gcaaataact
     3301 caacaagctg gtgacgcgta gcaattttga tatcctccgg gactcgttgt ccatcggtta
     3361 aataacccaa ggctaaattt gattcaataa cagcacttat ggctccacct actgcaagag
     3421 attcatccaa tttcgtaata atgcactgat ccacatgatt tgcctggtaa cgcttaatcg
     3481 catcgattaa tacctggtaa tgactggtag ctggtaatac caatactttt tctatagatt
     3541 gcagataatt acttaaaaca gtcatttgtt tggttacacg ttcatcagaa ggattcatac
     3601 cggctgtatc aatcaaaatt aattttttgt catttaattg acgcaatact cttgccaaag
     3661 agacttcatc ttgcgcaata cagactttaa ctcccaaaat cttcccatag agaattaatt
     3721 gttcctgagc tgctattcgg taggtgtcca tagtgaccaa acccaactta tcagcaccaa
     3781 atcgtaaagc aaagcgtgct gcaatttttg ctagagttgt tgttttacca actccagttg
     3841 gaccaacaaa cgcgtagata ccaccttctt ctattctact agaatcacga attggcaatt
     3901 gctctgtgac ttgtgacaaa ataaattgcc aggctctttg ttgattcaaa tcaggtttta
     3961 ctgagcgagc ccaaattgtc gccgtattgg gactaacacc taaatacaag agtttttgca
     4021 gcataactgt atgtaaaggc tcatgaatac caacatttcc acgcagctgt gcttcgagca
     4081 ttcctctcag tgtcaaaatt tcttgccgca tttcgtctaa tggcgtttgc gctgaatatt
     4141 tttctgttcg gcttgggggt tctgctatag ctacagcagc ttgagttgat atcactgttt
     4201 cgtcaaaatc aatagcagca actatttcaa caccatcttt tatgttatgg cttgacaaaa
     4261 taacggcatc agggccaaat gcagccttta tttgctgcat ggcactgcga gtatctgcag
     4321 cgacaaagcg ttttaatttc ataatgcctc ctgccttcaa cacacctatg caaccccaaa
     4381 aactaaccta cagttccaat tattcttatt tgtttgttat caggtatttc ctgataagac
     4441 aaaacatgaa agtcattgga tattgtacgc acaaatcgtg ccaaaactgt tcgtatactt
     4501 ggttgaacta ccaaaactga gctctcctgc tttgcctgtt gttgtgcagc gaactggatt
     4561 agtgcttgtt ggattttatc tgccattcct ggctcaaagc taactccgcc atgtccactg
     4621 ttttgtagag tattgtgcaa caattgttcc aactctggtt ttaatgtaat aatgggcaat
     4681 tcctcgttta atccactgat tttctgagtt atcaatcgtg ataaagcaat tctcacttgg
     4741 ctaatcaaat attccgtatc ttttgatttt gacgcatatt ccgccaaagt ttcgactata
     4801 gtgcgtatgt ctgttaatgg aatatgttct gataataatc cctgcaacac tttgctaacc
     4861 atacctaaag taagtccatc aggtacaagc tctttaacca gtttagggga acttatggcc
     4921 aacttatcca atagctgctg tgtttcctca tagccaagta attgctggct gttttgttcc
     4981 aggatcatgc ttaaatgagt agcaactact gttgatgcat caactactgt ataacctaaa
     5041 gtgtgtgctt tctccttttg attttcattt atccacacag catccattcc aaaagccgga
     5101 tctcttgttg ggattccctc taaattacca aatacctgtc cggggtttat tgctaaccat
     5161 ttatcaggat gaatcattga ttcccccata atcaccccag ccaaacttaa tctgtaatga
     5221 ttaggcttta aatctaggtt gtcacgaaca tgaacggatg gaattaagaa acccaactct
     5281 tgcgatattt tctttcggac acctttaatt cgcgaaagta atattccacc ctgagcatta
     5341 tcgaccatag ggatcaatcg gtaacctact tccaaaccaa tgacatcgac aggattaaca
     5401 tcatcccatg ataactcact cagtttagag tcactggttt gtgaaaattc attttgctct
     5461 ttatcctgct gctcacgttt aactttttcc tgattgataa ataaataagc aataccggct
     5521 aaccctgctg ataatagaat aaatgcaata tgaggcatac caggaattat acctattaga
     5581 ccaataatag ctgctgcaat gacaagtgat cttggttttg caaatacttg agaaataact
     5641 gcctgcccca tattttgggc gctagaaact cgggtcacaa tgattgccgc tgcagtagac
     5701 aataagaggg agggaacttg agcgaccaaa ccatccccga ctgtcaataa aatataatta
     5761 tgaactgcgt cattaaaatt catattgtat tgaaatatgc ctattattaa tccaccaacg
     5821 atgttaatga ttaaaaccaa aattcctgct atggcatccc ctcggacgaa tttactggca
     5881 ccatccattg aaccgtaaaa atccgcttcc tgagctactt ctgttcgacg cttgattgcc
     5941 tgttcctgat tgattagacc tgcattgaga tctgcatcga tagccatttg ctttccgggt
     6001 aaagcatcca aagtaaaccg cgcacttact tcagatactc tccccgcccc ttttgtcact
     6061 acaacaaagt taataacgac aaggataata aataccacta tacctactgt gtaattacct
     6121 ccaatcacca cttcaccaaa agattttatg acctgaccgg cagcatctgt accattatga
     6181 ccattgagta agactactcg tgtagaagct acattgagag ctaatcttaa caaagttgcc
     6241 acaagtatca ctgtgggaaa caccgcaaaa tccaggggtc tttcactata aataactgcc
     6301 aacaaaacaa ctaaagaaag agcgatatta aaagtaaaaa atatatccag taggaaagga
     6361 ggcagaggta acatcatcat gctgatcatg acaatcagta ttaatggagt accaaaccct
     6421 tggcgcatcc aatattctat ataattcata acattgttca ttcaatctcc tcctctgctt
     6481 cgcgagccag ttcaggtgga atagggacat tttgtaaaat ttcaggctta taatcataac
     6541 gttgcctgtc acgtagctga aaaatatagg ccagcacctg agccacagca acatataacc
     6601 ctctgggaat ttctgcattc agttttgttg aaaaataaat agcgcgagct agtgcaggaa
     6661 ctgaaataat agggatctta tgctctttag caaccttgga tatctgaaaa gcaaccaggt
     6721 ttttaccctt ggcaatcacc actggagcct tttttccttt cttttggtaa ctaagagcaa
     6781 ctgcatagtg cgtaggatta gtcaatatga cgtcggcttt aggtacttca ctcatcatcc
     6841 gtcgtcttgc agtctcctgc tgtgcgcgac gtatagcgct ttttacctca ggtttgcctt
     6901 ccgtctcttt gtattcatct tttaattcct gtttagtcat cttaatcgat ttactatgtt
     6961 catataattg gaatggaaca tctattgccg caattaatat cagacttgca gaaatcagca
     7021 caaaagattt taaaaggatc attaccccac tagtgattgc tatttctaat ggtgctttcc
     7081 ccaactcaag caacaagggt acttgcgatc taagcactag aatagatgcg ctcgccacca
     7141 ggacaaattt taaaaaagcc ttaagcatct caacaaatcc tttgagagaa atcatgcgct
     7201 taagcccttt aagtacattt aatcgagaaa attttggctg tatagactgt ccactaaaaa
     7261 cccatccccc cataagcaat ggtgctagca aagacaaaat aaaaataaca agcaatagtg
     7321 gaacaacaga ccaaagccct gattttgtta attgaaacaa attttctaaa gaattattcc
     7381 cggtaacaag aatgtcacga tcgaaatcaa acgcctgttg cattatactg gcaatttgca
     7441 gtgataattg tttcccaaaa ataaagaaac caagtccggt aaataataaa atgaccgttg
     7501 cattaaaatc tttggaacgt gcaacctgcc ccttttttcg tgattctttt aatcgcttgg
     7561 aagaaggctg ttccgttttt tcttgtgatt gttcttcatc ggccatcagc gcaatattcc
     7621 tgttataaat tggattccat gcttaatgct ctctaccatt tctgtcccga cagtgggcaa
     7681 ccctatttta atgatgacaa accccatcaa tagcgtaatc ggaaaaccta aagagaaaag
     7741 gtttaattgc ggagcaaccc gagccataac tccaaaagac aagcttacaa taagcaaaga
     7801 taaaatcgca ggtattgaaa tcaataccgc ttctttgaac atccaccctg aaaacataat
     7861 cgtactccag acaactgatg gatccagaga agatttaccg attggcatta ttctgaaact
     7921 ctcgattaga gtttcaagaa tagccaaatg gccattcaat gccaaaaata ttaatgtggt
     7981 caccattaaa tacaattgac tgattaaagg cacactagca ttgctggttg gatcaaccat
     8041 taccgcaaat cccaaacccg cctgcatgga tataatttgc cccccaagaa tgaatacttg
     8101 aaatactatt tgcaacacaa atcccattaa caaacccaat aataattcct gtacgagata
     8161 gacaagataa aggccattaa aattttcgaa agacaagtta tgaggcacat aggtaaaaca
     8221 cacacagctt aaagtaaaaa caaagagaat tttaatgcgt gccggtaata gtgctgaaga
     8281 taaaactggc accgtaagca tcaaaccacc aatacgcacc atgggccata taaattggct
     8341 cattaagcga atcatagttt gataatcgag attcattttt tatccaatca aataaggaat
     8401 attgcttatc aaagtatctg tataatttat caatgtcttt aataaccaag gtcctgctaa
     8461 aattagaacc aaaaaggtga caaataactt gggtataaag cttaaacttg tctcattaat
     8521 ttgagtcgca gcctgaaaca tcgatataat tagtccaaca attaatcccg gtaatatcaa
     8581 cactgccagt aatagcaaaa tgatataaac actatcacta aacaacgaaa cgactgattc
     8641 aggggtcata tagcctccta ggcaagagca aaacttgaag caagtgaacc caaaaccaaa
     8701 gtccatccat caaccatcac aaacaacatg attttaaaag gtaatgaaat aatcaagggt
     8761 gaaagcatca tcatccccat agccatcaaa acgctggaca caacaagatc aataattaaa
     8821 aaaggtaaaa atagaataaa gcctatctga aatgcggttt ttaactcgct agtaatgaaa
     8881 gccggaatga gaacactcat aggaatctca tctaaagttt ttggtacatt gtgagcaaat
     8941 ttttcaaata aagataaatc attttttctc gtttgattta gcataaactg acgaagcggc
     9001 ttttgcgcat tggataatgc cagagaaaaa tcaatccttt cattcatata gggctgtatt
     9061 gctgtttcat taattttgtt gaatacaggt gacataacaa aataagtcat aaatagagca
     9121 atgccgatta atatttgatt ggtaggcgtc tgagccatcc ctattgcctg ccgtaaaatt
     9181 gacagaacaa taattatcct tgtaaatgac gtcattgcca tcagcaatcc tggcaatacg
     9241 gtcaataaag tcataaaaat taaaatttgc agattgatac tataagattg cgaaccatca
     9301 gttccaggtt gcattgtaac aacgggaaag gataaagggt ctgtgtgacc tgcaacaggt
     9361 aataaaagaa ataatatcaa aatcagtaac caacttttaa ttttcattta tgattttcct
     9421 attgctgatt tcagcacatc ggcaaaagac
//
LOCUS       DATNSN010000128         9441 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.128, whole genome shotgun sequence.
ACCESSION   DATNSN010000128 DATNSN010000000
VERSION     DATNSN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.128"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            56..2710
                     /gene="vpdC"
                     /locus_tag="VAH76_002399"
     CDS             56..2710
                     /gene="vpdC"
                     /locus_tag="VAH76_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdC"
                     /protein_id="HEO1387000.1"
                     /translation="MMTPQHVISQLLESDTKYPQNLDLLKKIIISAYLGRLRINGLPP
                     DNKIALGNYLFDDERMIFDFTRLSDSKRALFIEWLLEPHQKDKEHGFLSGVYVNEYRG
                     FTAEVALSWWGILKNWLFNNKSEHWKITDLGLSVNYQLTGIEMCHGKQGILIGFNQFL
                     VPPSGTKYRDPNDNQSEPLGNVKRLFITDKLVDELVGLNYKNLNYESVCKSPHPQSID
                     VTDPEARHKDMYDYRKMQRFNGVRPWYIRLWRWISKLFFGDTYDAKKPSTTDDNLELL
                     YKTKTVKIYQRSKSKEILVTEKRPDITNLVFCGGGAKIFAHVGVWKALNEAGMKPTKF
                     AGSSAGAIMSLMCYLGYTADEISELFKHFKQEHLVQFDIDRNGLSDTHSLKTALDYAI
                     ANKVRQIVNKYKIPYPKGKITFSTLASLREQFPDCGIGKELIVTATNKRSGKTIYLSA
                     NCYPLLEVSEAVKISASFPVLYRDTLLDGEEHNDGGILSNFPTEVFIDDHSTLLESEF
                     GNNLKVLAVQFDNGVERNVIDRIMDKVYRENFVLNWIYSLLTGVSDPASGWERDRLKL
                     RQYALQTIIPETGKVSTTGFNVERQSQEELIKNGYQSTLDYLNVRYTRKNNSYVNKEI
                     MYSTFNSLGDLLAYCCYRGDKQWFNVVNNLIVQSTLPNRTALMKQSLELRKLYFNTSL
                     PSNPEKNKTACNESNTLTFFGNAVCQHHNHSQELENGNHDVLLALYPIFLKLSTDLLI
                     NKIDKNLLDRARHSLNIHSPFDCLEHFNKISGNAHVMIHIIVKLIKELKERSCQQIYD
                     VLKEVRHLLYSDINLMKANYFGKWDLSLPQCIRILNLLKQEKHDDVSRLLDCLRNQME
                     PVQTVKGGVHHDDLNNGGHERYRLGLGV"
     gene            2655..3434
                     /locus_tag="VAH76_002400"
     CDS             2655..3434
                     /locus_tag="VAH76_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO1387001.1"
                     /translation="MMTLITGATRGIGLALAYEFASHGHDLILIGRDSEQLEHISKQL
                     NTQYQVNVEHKVHDLSKPGSAFKLYTDLKELGIEVNCLVNNAGIGYMGSFVEMGMNKL
                     DDLMNINMISLSELTLCFLHDFVARNEGSILQVASTAAFQPGPFMAAYYASKAYVATL
                     SHAIAYELKDTKVSMSILCPGATKTNFFHASGMESSMLERGYIGMMTPEKVAKIAYKG
                     LKKGKLYIIPGLRNKILAYAAAISPKAIALRIASYLHHKIL"
     gene            3440..3943
                     /locus_tag="VAH76_002401"
     CDS             3440..3943
                     /locus_tag="VAH76_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEO1387002.1"
                     /translation="MRLQLMKITGFTLLETMLALALLLGLLLLGSWSFFSLIQNNERE
                     TLVNSIKTAIQYSKIQAIHLGHPVYLLPFGSNENWSRGMVLAELNRKSNKTELIHQWQ
                     WSSNSWNINWKGVDPNHRIIISNIPNRAMSNGKFILNNRRTNEKVVVTLNRLGRVRVG
                     VINKKSK"
     gene            4040..4576
                     /locus_tag="VAH76_002402"
     CDS             4040..4576
                     /locus_tag="VAH76_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEO1387003.1"
                     /translation="MSINYQSLLIADTKGPGLLKDWLEYQGSLTDKLKAMTGNAELER
                     LSQKWSIPNWWDKYVLCIQDKSILQREIVMKNQGIVYWYARSVIPQSCYALKPEFFNR
                     LENESIRNLIFDESSVRRLPILCYPIDQLNLEFHWVKKYIGSEYSQMWVRFTELVFQE
                     KSSFYLVEILLPELESLL"
     gene            4573..5421
                     /locus_tag="VAH76_002403"
     CDS             4573..5421
                     /locus_tag="VAH76_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEO1387004.1"
                     /translation="MIPWNAYVRLLRLNKPIGILLLWYPTAWALWMANQGFPSIELLM
                     IFLLGTVFMRSAGCVINDIADRHIDRHVARTQFRPLTSGEVSLSEAFILLFILLCASL
                     LLLLKLPINCFYFAVISVLITFLYPFCKRFLNAPQLILGLAFSMGIPMAFIASGKNLN
                     SDFIVLFLINFSWIIAYDTMYAMTDKADDLKIGVKSTAIYFASYDRLIIALLLIFLHS
                     LWLVWAINKNAEWFFYLLWCTAAGILTYQLKLIYARIPKNCFKAFLVSGYYGLVMWFA
                     VGLALI"
     gene            complement(5555..5953)
                     /locus_tag="VAH76_002404"
     CDS             complement(5555..5953)
                     /locus_tag="VAH76_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387005.1"
                     /translation="MKTKIIFKSISSLLLGLLLVSCSNELPPGEYDANEIGKIKKVIP
                     GVIISKRPVNLHRQNADANLHVKGGADSNLIDGGYNRTHGVEYVIKLNSGTIISVVQA
                     EDLKLKNKQKILVIYGRHTRVVADNGSDVY"
     gene            complement(6033..7805)
                     /locus_tag="VAH76_002405"
     CDS             complement(6033..7805)
                     /locus_tag="VAH76_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO1387006.1"
                     /translation="MKLWIMLFNCFIVTFLITFSHAKAPVNNNKSSYCLPNQPCWPTP
                     KEWEQLNKKINGHLIKARNYLSPCIEDPKSNGCKNTLEEIKNPFYLETQPTATQSTGW
                     FDAWDSEISPYIVAAKTAQEIAHAVKFARKHHIKLVVKGTGHDYLGRSNAPDSLLIWT
                     HHMREIQFQKHFVPNGCSANHKGVPAVTAEAGTRWIEAYKEVTVNQGRYVQGGGCTSV
                     GVAGGFIQGGGFGSFSKKYGTGAGGILEAEVIIADGSILIANKCQNQDLYWALKGGGG
                     GTYGIVSKITLETHELPATFGALTGSITAKTEQDFKNLIDYFIIFYRERLHNEHWGEQ
                     VILGPDNKMELALVFQGMSKTQVDRLWQPFKTWLSQKPEHYQFTINSLVLPARKFWDY
                     DYLSKNLPDHIVIDRRKNIPYPEFWWAGNQSEVSIYLTHYQSGYLPYTLFQEKFSNKL
                     SNALFEASRLTKLSLHFNKGLAGASQQAIERQRKTSMNPSVLDAAALITIIGGQQYVF
                     PKLPGHKPDLDKARFSAKQAKKAMKLISALNPDSGTYGNEADYNLKNWQRALWGSNYE
                     KLLQIKHHYDPENLFNCHHCVGGE"
     gene            complement(7859..8623)
                     /gene="deoC"
                     /locus_tag="VAH76_002406"
     CDS             complement(7859..8623)
                     /gene="deoC"
                     /locus_tag="VAH76_002406"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO1387007.1"
                     /translation="MSLDKHFNDVLDKLLSNQDIISISNAQLMHCIDLTLLEADASLE
                     ALHQLQSQACQNNVAAICVLPKHLHVFKPNSKIKLATVINFPLGNDDLLVSLAEIDNA
                     IQLGANEIDYVLPYQYYLRGNKQKALNHCDVIIQTCKKHQLALKIILETGLFPDMESI
                     YQLSSEIIALGGRFLKTSTGKTPQGATLAAVFAIVSAVMDSTYASSCGIKVSGGIKTP
                     LQAQQYAQLAELLMGKAIHKDWFRIGASTLLEKLLS"
     gene            complement(8620..9438)
                     /locus_tag="VAH76_002407"
     CDS             complement(8620..9438)
                     /locus_tag="VAH76_002407"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO1387008.1"
                     /translation="MSYPHLAAEYIQKTCPSFKPKVGVVLGSGLGQFAEELEDTVAIE
                     YEKLPGFPRTTVQGHGGKLILGYYGSTAVICLQGRAHTYESMENHEAVKTYVRTLKLL
                     GCQYFIATNASGSLKEEVGPGELMLITDHINFQPGNPLVGPNDDEFGPRFYPLDNAYD
                     ITMRNALLDIAQRHSIKLHQGVYISVLGPNYETAAEIRAFKLLGADAVGMSTVPEVLV
                     ANHCGLRVAVIATITNYATGLAKTSHSHESVVKMASSAAEKLNTLIKQYILELS"
BASE COUNT         2923 a         1637 c         1780 g         3101 t
ORIGIN      
        1 taatccgtat tatttcagat atttattcta tggactaccc agacgtaagg atttactgat
       61 gaccccacag cacgtcataa gtcaactgct ggaatctgac accaaatacc ctcagaattt
      121 agatttatta aaaaaaataa tcatatcagc ttatctggga cgcctcagaa ttaatggatt
      181 acctccagat aacaagattg cacttggaaa ttatttattt gatgatgaac gtatgatttt
      241 tgatttcact cgattgagtg attcaaaaag agctttattt attgaatggt tgcttgagcc
      301 tcatcaaaaa gataaagagc atggtttttt aagcggcgtt tacgtgaatg aatatcgagg
      361 tttcactgcg gaggttgctt taagctggtg gggtatctta aaaaactggt tatttaataa
      421 taaatctgag cattggaaaa taacggattt gggactttct gtcaattatc agctgactgg
      481 tattgaaatg tgtcatggaa agcagggtat cttaattgga tttaaccagt ttttagtacc
      541 accttcaggt acaaaataca gagatccgaa tgataaccaa agtgagccat taggtaatgt
      601 taaaagatta ttcattactg ataaactggt tgatgaactt gttggactca attataaaaa
      661 tcttaattat gagtctgtct gtaaaagccc tcatccgcaa tctattgatg taactgatcc
      721 agaagctcgt cataaagaca tgtatgatta tagaaaaatg caacgtttca atggtgtgag
      781 gccttggtat atcagacttt ggagatggat atctaaacta ttttttggcg atacatacga
      841 cgctaaaaaa ccaagcacta cagacgacaa tttggaatta ctttataaaa ctaaaactgt
      901 caaaatttat cagcgctcca aatcaaaaga gatcttggtc acagagaagc gacctgatat
      961 tactaatttg gttttttgtg gaggtggggc aaaaattttt gctcacgtgg gggtttggaa
     1021 ggctctcaat gaagcaggaa tgaagccaac caaatttgca ggaagctctg ctggtgcaat
     1081 catgtctttg atgtgctacc taggttatac tgctgatgaa attagtgaac ttttcaagca
     1141 tttcaaacaa gagcatttag ttcaatttga tatagacaga aatggattat ctgataccca
     1201 ttccttaaag accgcgctgg attatgctat cgcaaataaa gtaaggcaaa tagtaaataa
     1261 atataaaatt ccatatccta agggaaaaat aacttttagt actttagcgt ctctaagaga
     1321 gcaatttcca gattgtggaa taggcaaaga attaattgtt acagcaacaa ataaacgctc
     1381 aggaaaaact atttatttat cagcgaattg ttacccatta ttggaggtca gtgaagcagt
     1441 taaaatttct gcgagctttc cagttttgta tagagatact ctgctggatg gtgaagagca
     1501 taatgatggt ggaatattaa gtaattttcc aaccgaagtt tttattgatg accactcaac
     1561 gctgttggag tctgaatttg gtaataattt aaaagtattg gcagttcaat ttgataatgg
     1621 ggttgaaaga aatgttattg atagaataat ggacaaagtt tatcgagaga atttcgttct
     1681 taactggatt tatagcttac tgactggagt gagtgatcct gcaagtggat gggaaagaga
     1741 ccgccttaaa ttaagacaat atgctcttca aactataatt cctgaaacag ggaaagtatc
     1801 aacaacagga tttaatgtag agaggcagag tcaggaggaa ttgatcaaaa atggttatca
     1861 atcaactctg gattatctta atgtaagata tactagaaaa aacaacagct atgttaacaa
     1921 ggaaataatg tactctactt tcaattcttt gggagatttg cttgcttact gttgttacag
     1981 aggagataaa caatggttta atgttgttaa taatctaatt gtgcaatcta ccttacctaa
     2041 tcgtactgct ttaatgaagc aatcccttga acttagaaaa ctttatttta atacgtctct
     2101 accatctaat cctgaaaaga ataaaacagc gtgtaatgaa agcaatactt taactttttt
     2161 tggtaatgcg gtttgtcagc atcataatca cagccaggaa cttgaaaatg gaaaccatga
     2221 tgtcttactg gcgctctatc caattttttt aaaactatct actgatttat taataaataa
     2281 aattgataag aacctattag accgtgcacg tcattcacta aatatacatt ctccctttga
     2341 ttgtcttgag cattttaaca aaatttcagg aaatgctcat gttatgattc atatcatcgt
     2401 taaattaatt aaagagctca aagaaagatc atgccaacaa atttatgatg tattaaaaga
     2461 ggttaggcat ttattgtata gcgacattaa tcttatgaaa gcaaattatt ttggcaaatg
     2521 ggatttaagc ctccctcaat gtatacggat cttgaatttg ttaaaacagg aaaagcatga
     2581 cgatgtatct cggttattag attgcctacg taaccaaatg gagcctgtgc aaacagtaaa
     2641 aggaggagtg catcatgatg accttaataa cgggggccac gagaggtata ggcttggcct
     2701 tggcgtatga gtttgcatca catggccatg atttaatact tattggtaga gattctgaac
     2761 agctggagca catttcaaag caattaaata cacaatatca agttaatgtt gaacataaag
     2821 tacatgactt aagtaaacct ggaagcgcat ttaagttgta tactgattta aaggagctgg
     2881 gtatagaagt caactgcctg gttaataatg cgggcatagg ctatatgggt tcttttgttg
     2941 agatgggcat gaacaagctc gatgatttaa tgaacataaa catgatttct ttaagtgaat
     3001 taacactatg ttttcttcat gattttgtag ctcgaaacga aggcagtatt ttacaggttg
     3061 cttcaacagc agcatttcaa ccgggtcctt ttatggcagc ctactatgcg agtaaggcat
     3121 atgtggcaac gctttcccat gctattgctt atgagcttaa agatactaag gtatctatgt
     3181 ctattctttg tcctggagca acaaaaacca attttttcca tgcttctggt atggaaagtt
     3241 ctatgttgga gaggggatat attggaatga tgacccctga aaaggtcgct aaaattgcct
     3301 ataaaggatt aaaaaaagga aagttgtata ttattccggg gctaagaaat aaaattttag
     3361 cctatgccgc ggccatatca cccaaagcga ttgctcttag gatcgcatct tatcttcatc
     3421 ataaaatttt gtagatgata tgcggcttca attgatgaaa ataacaggat ttactctact
     3481 tgaaacgatg cttgcattag ctttgctttt aggattattg cttttaggtt catggtcatt
     3541 tttttcacta atacaaaata atgagagaga aacattagtt aacagtataa aaacagccat
     3601 tcaatactcc aaaatacagg ctattcattt ggggcatccg gtttatcttc ttccttttgg
     3661 ttctaatgaa aattggtcca gggggatggt gctcgccgaa cttaatcgaa aatctaataa
     3721 aaccgagctt attcaccaat ggcaatggag ctctaattcc tggaatatta attggaaagg
     3781 cgtagatcca aaccatagaa ttattatatc caatattccg aatcgtgcga tgagtaatgg
     3841 taaatttatt ttgaataata ggcgaaccaa tgaaaaagtt gtggttactt taaataggct
     3901 tggcagagtt agggtggggg taattaacaa aaaatcaaaa taaaataatt ttgtttttat
     3961 cttaaattat aagcaatggt tgttcataaa gaggtaattt tgtaatatct tatgaaattt
     4021 ttttccaaat tatttatgta tgtctattaa ttatcaatcc ctgctgattg ccgataccaa
     4081 aggaccagga ttactcaaag attggttaga ataccaaggt tccttaacag ataaactaaa
     4141 agcaatgacc ggtaatgcgg agctggaacg tttatctcag aaatggtcta tcccaaattg
     4201 gtgggataag tatgttctgt gtatacagga caaaagcatt cttcaacgtg aaattgttat
     4261 gaaaaatcaa gggattgtct attggtatgc gcgatcggtt atcccacaat cgtgttatgc
     4321 ccttaagcca gaatttttca accgtttgga aaatgaatca atacgaaatc tcatttttga
     4381 tgaaagtagt gtgagacggt taccaatact ttgttatcct atagatcaat taaatcttga
     4441 gtttcattgg gttaaaaaat acattggctc tgaatactct cagatgtggg tcaggtttac
     4501 ggagctagta tttcaggaaa agagttcttt ttatttggtt gaaatcttgt taccggagtt
     4561 ggaaagttta ttatgattcc ctggaatgcc tatgtcagat tattacgctt aaataaacca
     4621 ataggaattt tattactttg gtatcccacc gcatgggcac tttggatggc aaatcagggc
     4681 tttccttcta ttgaattgct tatgattttt ttattaggta cagtattcat gcgttccgca
     4741 ggttgtgtta ttaacgatat tgctgacagg catattgata ggcatgtagc ccgaacccaa
     4801 tttcgtccct tgacttctgg agaggtcagc ttgtctgagg catttatttt attgtttata
     4861 ttactttgtg cctcattgct tttattatta aaacttccaa tcaattgttt ttattttgcg
     4921 gttatttccg tgctaattac ttttttatac ccattttgta aacgattttt gaatgctcca
     4981 cagctgatat taggcttggc tttttccatg ggtattccta tggcatttat tgcctcagga
     5041 aaaaatttaa atagcgattt tattgtattg tttttaatta atttctcctg gattatcgct
     5101 tatgacacga tgtatgcgat gactgacaaa gccgatgatt taaaaatagg agtcaaatcg
     5161 actgcaatat attttgcaag ttatgacaga ttaattattg ctttgttact gatttttcta
     5221 cattctttat ggttggtatg ggcaataaat aagaacgcag aatggttttt ttatttgtta
     5281 tggtgtaccg ctgctggaat attgacttat caactaaaat taatttatgc aagaatacct
     5341 aaaaattgtt ttaaagcatt tttagtgagt ggttattatg gattagtaat gtggtttgct
     5401 gttggattag cattgattta aataatatgt cctctcaacc ctaatataaa atttgttgtg
     5461 ccaagagatt atgggtgtcg gaaaattcct tcataagaca ggctttagaa gaaaaaatcc
     5521 aacaccaacc catactttga agcaggaagg ctaattaata tacatcgctt ccattatcag
     5581 ctacaactcg agtgtgtctt ccataaatta ccagaatttt ttgcttgttc tttaatttca
     5641 agtcttctgc ttgtaccact gaaataatgg tgccagagtt taacttgatg acgtattcga
     5701 caccatgtgt tctattataa ccaccatcta tcaaattaga atcagcacca ccttttacat
     5761 gcagattggc atcagcgttt tgacgatgta aattaactgg tcgtttggaa ataatcacac
     5821 ccggaataac cttttttatt ttgcctatct catttgcatc atattcgccg ggaggaagtt
     5881 cattgctaca acttacaagt agcaaaccca atagtaatga tgaaatggat ttaaatatga
     5941 ttttagtctt catgataatt taaactccaa tgctaattaa ttcactatga ctagttattc
     6001 taactgagat tctcaaaatt attaattaat ttttattcac ctccaacaca atggtggcaa
     6061 ttaaacaagt tttcgggatc ataatggtgt ttaatttgta ataatttctc ataattggat
     6121 ccccataaag ccctttgcca attttttaaa ttgtaatcgg cctcatttcc ataagtgcct
     6181 gaatcaggat tcagagcaga tattagcttc attgcttttt ttgcctgttt agctgaaaac
     6241 cgtgctttat ctaagtcagg tttgtgccca ggcaacttag gaaagacata ttgttgcccc
     6301 ccaattattg ttatcaaggc tgctgcatcc aatacggaag ggttcattga tgtttttctt
     6361 tgtctctcga ttgcttgctg agacgcccct gccaagcctt tgttaaaatg taaagacagt
     6421 ttggtcaggc gagatgcttc aaacaaagcg ttagatagtt tattcgaaaa tttttcttga
     6481 aagagggtat agggcaaata accggattgg tagtgagtta aatagataga aacttctgac
     6541 tgattaccag cccaccaaaa ctctggataa gggatatttt ttcgcctatc aattacaata
     6601 tgatcaggta gatttttgct tagataatcg taatcccaga attttcttgc aggtagtacc
     6661 aggctgttta ttgtaaattg ataatgttca ggtttttggg ataaccaagt tttaaatggt
     6721 tgccataatc tatcaacctg agttttgctc atcccttgaa aaaccaatgc cagctccatt
     6781 ttgttatcag ggcctaatat aacttgttct ccccaatgtt cattatgaag tctttccctg
     6841 taaaaaataa taaaataatc tatgagattc ttaaaatcct gctctgtttt tgctgtaatg
     6901 cttcctgtta atgctccaaa tgttgctggc aattcatgcg tttccaatgt gattttactt
     6961 acaataccat aagttccgcc gccaccgcct ttcaatgccc aatacaaatc ctggttttgg
     7021 catttattgg caatcaaaat tgaaccatca gcgataatta cttctgcttc taaaattccc
     7081 cctgcaccgg ttccatattt tttagaaaaa ctcccaaatc ctccaccttg aataaatccc
     7141 ccagcaactc caacggaagt acagcctccg ccctggacat agcgcccctg gtttacagta
     7201 acctctttat aagcctctat ccatctggta cctgcttcag cagttaccgc aggaacaccc
     7261 ttatgatttg cagaacaacc atttgggacg aaatgtttct ggaactgtat ttctcgcatg
     7321 tgatgtgtcc aaatcagcaa agaatcagga gcattggatc ttccaagata gtcatgacca
     7381 gtacctttga caactagttt gatatgatgt tttctagcaa attttactgc atgagcaatt
     7441 tcctgcgctg tttttgctgc aactatgtat gggcttattt ctgaatccca tgcatcaaac
     7501 cagccggtgg actgggttgc ggtaggttgt gtttctaaat aaaagggatt ttttatttcc
     7561 tccagggtat ttttacaacc attacttttg ggatcttcaa tgcaaggtga cagatagttc
     7621 ctggctttta ttaaatggcc atttattttt ttgtttagtt gctcccattc tttgggggtt
     7681 ggccagcatg gttggttagg gaggcaataa gatgatttat tgttatttac tggcgctttt
     7741 gcgtgagaga atgttattaa aaaggtaaca ataaagcagt taaatagcat tatccataat
     7801 ttcattgcaa gacatctccc tgtttgcatg ctgacatatt aacacataga ttgtgcgttc
     7861 atgacaataa cttctcaagc agagtactgg ctccaatacg aaaccaatcc ttatgaatcg
     7921 ctttgcccat aagtaattca gccagttgag catattgttg agcctggaga ggcgttttaa
     7981 tccctcctga aaccttaatt ccacaagagg atgcataagt tgaatccata acagcactga
     8041 ctatagcaaa tactgctgcc agagttgcac cctgaggggt ttttccagtc gaagttttga
     8101 gaaaccgccc accaagagca atgatttctg agctaagctg ataaatgctt tccatgtctg
     8161 ggaaaaggcc agtttctaaa attattttta aggcaagttg atgttttttg caggtttgaa
     8221 taatgacatc acaatgattt agcgcttttt gcttatttcc tcttaagtaa tactgatagg
     8281 gtagtacata atctatttca ttagcaccca gttgaatggc attatctatc tctgccaagg
     8341 aaactagcaa atcatcattc ccaagaggaa aattgataac agttgctaat ttaattttgg
     8401 agttaggctt aaaaacatga agatgtttgg gtagtacgca aatagctgca acattatttt
     8461 gacaggcttg gctctgtaat tgatgcaatg cttcaagaga agcatccgcc tctagtagtg
     8521 tcaaatcaat gcaatgcatc aactgagcat tcgaaataga tataatatcc tggtttgaca
     8581 ggagcttgtc caagacatca ttaaaatgct tatccaaact cacgataatt ccaaaatata
     8641 ctgtttaatc aaagtattaa gtttttcagc ggcactagaa gccattttga caacggattc
     8701 atggctatga ctggtttttg caaggccagt tgcataattt gttatggttg caatgacagc
     8761 gacacgcaag ccacaatgat tggcgactaa aacttcagga acagttgaca tccccactgc
     8821 atcagctccc agcaatttaa aggcacgaat ttcagcagct gtttcatagt ttggtcccaa
     8881 gacagagata taaaccccct ggtgtagttt aatcgaatgt ctttgagcaa tatccaataa
     8941 tgcattacgc atggtaatgt cataagcatt atctaaagga taaaacctgg gtccaaactc
     9001 atcatcattt ggaccaacca ggggatttcc gggttgaaaa ttgatgtgat cggtaattag
     9061 cattaattca cccggtccaa cttcttcttt aagtgaacca gaagcatttg ttgctataaa
     9121 atactggcaa cctaatagct ttaaagttct tacataggtt tttactgctt catgattttc
     9181 catgctttcg taagtatggg ctctgccttg taagcaaata accgcagtag atccataata
     9241 tcctaaaatt aattttcctc catgcccctg tactgtagtt ctgggaaatc ctgggagttt
     9301 ttcgtattca atagcaactg tatcttctaa ctcttcggca aattgaccta accctgagcc
     9361 taatactaca cccacttttg gtttaaatga tggacatgtt ttttgaatat attctgctgc
     9421 taaatgtggg taactgatag t
//
LOCUS       DATNSN010000129         9428 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.129, whole genome shotgun sequence.
ACCESSION   DATNSN010000129 DATNSN010000000
VERSION     DATNSN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.129"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..109
                     /locus_tag="VAH76_002408"
     CDS             <1..109
                     /locus_tag="VAH76_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEO1387009.1"
                     /translation="TFLAFPNYKTILRYNNSIYYTGAIGYMADKICQGQ"
     gene            complement(257..448)
                     /locus_tag="VAH76_002409"
     CDS             complement(257..448)
                     /locus_tag="VAH76_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387010.1"
                     /translation="MRNKDEKAKEVVTDSRCAEANRLTHLITGAILADNCFFPSGAYS
                     IKPMVSNSQPPREKTTPSM"
     gene            716..1609
                     /locus_tag="VAH76_002410"
     CDS             716..1609
                     /locus_tag="VAH76_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387011.1"
                     /translation="MKDKTIPGPDVKPFNKEEFSENVAKGAIVGATSTTMTFGLFDKP
                     LAIATKASSEVAQHPWRGIKNLFAGAINGNFSTSWNNSLYVQAYRAYLEHPVKGYPVA
                     LLNSCLKNVVFFPAKYGSERALYALSGNEQQAKNYSGFVAGLMTVYLTAPVSVVKARL
                     MTNVPLNTLSLKRLGSGVNAIAMRDCIQYGIYFNAKERLNEKIGDGVMAGGIAGIVGY
                     VFSNPLSVIGLNQKTAVESVNIPNMAKRIYQANGVKGFYPLVGLAAWGMFARGVAIHQ
                     GTKLYESLQENEAYGRPTRNR"
     gene            complement(2011..3498)
                     /locus_tag="VAH76_002411"
     CDS             complement(2011..3498)
                     /locus_tag="VAH76_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2912 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387012.1"
                     /translation="MSYSKIDALERKWSSLEAIVKSYTDPVEYHLPEIKKLMTAEFVV
                     DFSSHAIYLHTEYVHRFTQQLINLRNTSPNDERLLYIHQLWTDLLNCRNQNPLKIKHH
                     KKLDGECTFNFSLKMMEGKPYGFKEVQILDPNNTAELNKAMGEIKRIDSLAHGANFGD
                     LPFIGDIVLSSMLHYKETICFIAKDKNDEVIGYCWGMMLRDIPAGEKNKVNVFWVMNL
                     AKHPDFYDPNIKVGEALRQHLANTLSKNPDCHFLGYEHVLNHNFHLAIVDGGIPKKEE
                     TISIGQNQYQAKSLVKYEPLKMQYDRKHIIKTNNHQYPYPKVDVIKSKFFTALWYAAP
                     TAFDFILGGILIYGRAYYQQWTHSMLGQPVTHRIEKPIPHEQQICDMNILKRIILSDV
                     WGQQGTTLFFDACIPDTIQKLQQLVKEDQIEFASLQQCVAKSGWALCRSKLTAYFYFA
                     ITQSQYPSAAINLLIQNDITPKKWIELISEERQIFAQQSSERASI"
     gene            4737..6671
                     /locus_tag="VAH76_002412"
     CDS             4737..6671
                     /locus_tag="VAH76_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEO1387013.1"
                     /translation="MHVLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQKEV
                     LHSSDTDFDRCAFLSVFNKKTNETFEAVVNLNSKKVVEWKQIVLEVPPYGKPPILIED
                     FQKCEQIVKADPTWRAAMEKRGLDDEQIDNIQVDAFSAGYFDEEEEREQRIVKGISFY
                     RDNLKDNGYARPIEGVVAIVDLGNEKVLRVIDDGRNVPIPKEKINYDSDSYPEKRKDL
                     KPLDIIQEEGPSFKVNGWEVNWQNWSFHVGFTPREGLVLHQIKYLDEGKERPIIYRAS
                     VTDMIVPYADPSVSHYWKSAFDAGEYGLGKLANELKLGCDCLGNIYYFDVPAADDFGN
                     ARLIKNAICMHEEDAGTLWKHYETRNSTFEVRRARELVVSFFTTIGNYDYGFYWRFGQ
                     DGSLKLEIKLTGIVQTAAIFPEAQYEWGGKLTPELAAPTHQHFFNARLHMMVDGEKNS
                     FSEHEFYPIAMGGKNPYGNAFGSSQKTFEHEGEAARSACAQTQRTWKIFNPNSVNGIG
                     TAPAYKLELPETPLLLADEESYIYKRGGFASKQVWVTQYDPQEKYAAGDYPNQSSGGC
                     GLPSYIKQNRKIDNDNLVLWVTLGSTHFPRPEDFPVMPTSIISAKLQPFGFFKRNPAM
                     DLPQGKVQEKGDTCCSSLTY"
     gene            complement(6719..6907)
                     /locus_tag="VAH76_002413"
     CDS             complement(6719..6907)
                     /locus_tag="VAH76_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEO1387014.1"
                     /translation="MADIINLNKKRKAKVRLEKEIKASENRIKFGRTKKEKQQEKQDN
                     ERSERHLDGHKLDKKEEN"
     gene            7568..8437
                     /locus_tag="VAH76_002414"
     CDS             7568..8437
                     /locus_tag="VAH76_002414"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HEO1387015.1"
                     /translation="MSDYLDKTASLTPATKRIVCDKATEYPHTGSYNQVATHGTYLCR
                     RCGLALFRGISQFSSGCGWPSFDDEIANAVARKPDADGQRTEILCARCDAHLGHVFTG
                     EYMTYKNLRHCVNSASLDFVADNEVLDTEEAILAGGCFWGVEYYLKRIPGVLKVESGY
                     TGGHVSEPSYEQVCSGTSGHYEAVRVVYDAAKTNYHDIVKRFFEIHDPTQADGQGPDL
                     GQQYLSAVFYFNQEQRQEAELLIQLLKKKGYQVVTQLLQAQPFWPAEDYHQDYYFKHH
                     KIPYCHKPVDRFG"
     gene            complement(8461..9426)
                     /locus_tag="VAH76_002415"
     CDS             complement(8461..9426)
                     /locus_tag="VAH76_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387016.1"
                     /translation="MAFSKSETTVDKFSLDEFPAFSAHDLEMAFSLKKKYRESKESGY
                     PFTGFVCDYERREKFYDFIYSSIHSPKLFENNFKVQIAYLDREKSGVRHWSLIELQYS
                     IEKGKNTLKILVLDSLGAQLSYNTAQILANEVGFQDLLDENEALSIKIYIPDVKLQHS
                     RAGCATFTMDCASMLSTQDKYENIYDFMDKQAEVSDKVKIAKLDTDFPVHCASLPPRI
                     LRGAQSRAIITDDKVLNSTPLINSKEETFKHSLLKHCSFFEDQPQTPENIKRNERIMK
                     KALKWRNTIDLKMKTSSAEEFESTCAIDDLEEYANSCLPSKSVQL"
BASE COUNT         2793 a         1752 c         2012 g         2871 t
ORIGIN      
        1 gaccttcctg gcgtttccca actacaagac gatactgcgt tataataact ccatttatta
       61 tactggggct atcgggtata tggccgataa aatttgccag ggtcaataaa tagtatgctg
      121 atggctagat aataaatgat gaagagttct tttggtgaaa tgaataaaag aacccttttt
      181 attgagcgac tcttaaaaaa gccatttgct ttattctgtg cttttggcaa gtggcatgat
      241 cgcattcagg ctttgtttac atgctaggtg tagttttttc ccgaggcggt tgtgagtttg
      301 ataccatagg ttttattgaa taggcgccgc ttgggaaaaa acaattgtca gcaaggattg
      361 cccctgtaat caggtgggta agccgattgg cttcggcaca tctcgaatca gtgacaacct
      421 ctttcgcttt ttcatcttta tttcgcataa caatcctgtg aagttaatct ttgcagagga
      481 caccatgatg gtttcatgtc atacaaacga agcaaccggg taccgagttg agtccaaagc
      541 tcgatcttga ttccttcgct aacgcttgca acaaggagta tttcatttgt agtcccttgc
      601 gttggtgcaa agcaagttaa gttaaaaata atcgaccagt taatgtctgg ttaaggatag
      661 cattatatca ttacagatca attgcgcacg tcgtatgatt taagagtaat aagtaatgaa
      721 agacaaaaca atacctggcc ctgatgtaaa accatttaat aaggaagagt tctctgaaaa
      781 cgtggcaaaa ggagccattg ttggcgcaac cagcacgacc atgacgtttg gtttattcga
      841 caagcccttg gccattgcta cgaaagcctc ctcggaagtg gctcagcatc catggcgcgg
      901 tatcaaaaat ttgtttgccg gggcgatcaa cggaaatttt tcgaccagct ggaataatag
      961 cctgtatgta caggcttatc gagcttattt agagcaccct gtcaaaggtt atcctgttgc
     1021 tcttttaaac tcctgcctaa agaatgtggt gttttttccc gcaaaatatg ggagtgaaag
     1081 ggcgctttat gcgctttcag ggaatgaaca gcaagcaaaa aactattccg gattcgtggc
     1141 cgggctcatg accgtttacc tcactgcacc tgtttctgtt gtgaaagcaa gactgatgac
     1201 taacgtacct cttaatacgc tcagtttaaa acgattgggc tcaggcgtga atgccatcgc
     1261 gatgcgtgac tgcattcaat atggaattta ttttaatgca aaagagcggt taaatgaaaa
     1321 aattggtgac ggtgtcatgg cgggtggcat agctggcatt gtggggtatg tctttagtaa
     1381 tcccttaagt gtgattggac tcaatcaaaa aacggctgtt gaatcagtaa acattcccaa
     1441 catggcgaaa cgaatttatc aggcgaatgg tgtgaaaggg ttttatcctt tagtcggcct
     1501 ggccgcttgg ggcatgtttg ctcgtggcgt ggctatccat cagggcacta aactatacga
     1561 atccttacag gaaaacgaag cttatggaag accaacaagg aacaggtagt aaattcatga
     1621 cgccccctgt ttccatgaaa atatttcagg ggggcaggac gatgaattgt attggacaat
     1681 aaggctataa cgcggtgacg gtttaataat agtcgccgta ttgactcaat tacgagcaag
     1741 cgtagcaact gtcttgaatt agaaagagat tttatctcgg aacgaaccaa agaaggattg
     1801 cgtgcccgtt ctgcaaaggg tattaaactt ggcaaaccca aagggggcat ccaggcagcg
     1861 atgtatgata aagataagga aaaaattatt tcaactcact tagcttatgg caaatattta
     1921 tccctcaaag cgtttctcaa taagccattg ttaccatgaa ccagggtaag ctttaatttt
     1981 cttaaacaaa atggagtatt tagtcctccc ttagatggaa gctctttcac tgctttgctg
     2041 agcaaatatt tgacgctcct cactgattaa ttcaatccat tttttaggtg taatgtcgtt
     2101 ttgtatcaat aagttgatcg ctgcactagg atattgactt tgtgtaatgg caaaataaaa
     2161 gtaagcggtc aatttgcttc tgcataaagc ccaaccactt tttgcgacac attgttgcaa
     2221 actagcgaat tcaatttgat cttctttgac taattgttgc agcttttgaa tcgtgtcagg
     2281 gatacatgca tcaaaaaaca atgtggtccc ttgttgtccc catacgtcac ttaatataat
     2341 tctttttaaa atgttcatgt cacaaatttg ttgctcatgg ggaataggtt tctcaattct
     2401 atgtgttacc ggttgcccca acatggaatg tgtccattgc tgataataag cccgaccgta
     2461 aattaatata ccacccaaaa taaaatcaaa ggcagttggg gctgcatacc aaagagcagt
     2521 gaagaatttg gatttgatga cgtccacttt ggggtaggga tactgatggt tattggtttt
     2581 aatgatatgt ttgcgatcgt attgcatttt taaaggttca tatttaacca aggatttagc
     2641 ctggtattga ttctggccaa tacttatcgt ttcttccttc tttggtattc ctccatcgac
     2701 tattgctaaa tgaaagttat gatttaaaac atgttcataa cccagaaaat gacaatcagg
     2761 atttttagat aaggtatttg ctaaatgttg tcgcaatgcc tccccgactt taatattagg
     2821 atcataaaaa tctggatgtt tggctaaatt cattacccaa aatacattga ctttattctt
     2881 ttcaccagca ggaatatctc gtaacatcat tccccaacaa tacccaataa cttcgtcgtt
     2941 tttatccttg gcaataaaac aaatagtctc tttataatgt agcattgaag ataggacgat
     3001 atcccctata aaaggcaaat cgccaaaatt tgctccatgt gctaaagaat cgatacgttt
     3061 aatttcaccc attgctttat taagttcagc agtattattg ggatctaaaa tctgaacttc
     3121 tttgaagcca tagggcttgc cttccatcat ttttaacgaa aaattaaagg tacattcgcc
     3181 atctaacttt ttgtggtgtt taattttaag aggattttga tttctgcaat ttaacagatc
     3241 tgtccataat tgatgaatgt aaagtaatct ttcatcattt ggtgaggtat ttcgcaaatt
     3301 gattaattgc tgggtgaaac gatgcacata ttctgtatgg agataaatag catggctact
     3361 gaaatcaaca acaaactctg ccgtcattaa ttttttgatt tcgggtaagt gatattcaac
     3421 aggatctgta tatgatttta ctattgcttc tagtgaactc catttgcgct caagtgcatc
     3481 gatttttgaa tacgacatct atatttcctt gtaataaatt ttcggagaaa tcttaacatg
     3541 gcaatttact tggccaaatc ctatttggtc aataaagagt aggctctttt taaatttaaa
     3601 aaggaactat ctttatatgg aaaaactata actctcgaga cgggagtttt cattgtgctg
     3661 tgttgacatt agatagagat catcagcgat ggggtgaggg ccttggatcg gattcctatt
     3721 gacacaagct ttctaaagga atagttgaga atttagagta cagatatctt tcattgccag
     3781 tttgggctgg atatgcggga aaacaaagcc ttacaataca ataaggttgg tcgaggggga
     3841 attgaacgcc acaatccatg tggcttccat cttgcctgat gctccaattc cgcctcctcg
     3901 tttttttatc ataacaaatt ccagaatgga gtcacgggta taaattctga atggtgtgta
     3961 agatatttct cattctctgc tacttagatg atcataaata gccaggttgt attatttatt
     4021 gttttacgat atttccagtc taaagaaatc tatctatttc aatctcttta ttatgatttc
     4081 cactttgcaa aaagaacaag catccttcta agtgccggaa tttagggtat aaccagagtg
     4141 tattcacaga gttatccaca gaaaatgggg ataagtgatt aatgcaatcc aggtatcaat
     4201 acctaataag tccttgtagg gcaaggggtt ttgtaaaatg tgtgggatct caggcggtca
     4261 gagattgaaa aatagaaata gttattcaca ggggggatat gcaatttaga tgttaagcga
     4321 tttacatcgt aacaaaaaaa aaaataaaaa aacagtcttg acgaagtcga tttttttcca
     4381 aaaaaatttg ggcatgctgc gattacgtat aaaccgatta agccagtgtg gtaagtgcat
     4441 ggacaactat ttgattcaag ccgattagaa tgatataaag tgcaccttta atggttgcat
     4501 cgcctaatgt gagcgccagg agattaaata atataccacc tttaaaaggc ccgactttta
     4561 atttgacaag cttatgttgt attcactaat taaaaaatac cataccgcac accgcctatc
     4621 cgactattct tagcgacata ccgcttttgt atttctatct aattacttca acgcgctgtc
     4681 tctttagcta acgcaaagag caatatcggt ttttttaata tgtaaaggat gtcaatatgc
     4741 atgtgttaga tcccttaact cttgaagaaa ttcaggaaag ttgtgatatt ttaaaacgag
     4801 aaaaagaact gtctgacagt tatcggtttg cctgggtaat gacttacgaa ccgcctcaaa
     4861 aagaagtttt gcattcaagt gatactgatt tcgaccgttg cgcttttctc tcggttttta
     4921 ataaaaaaac gaatgaaacc tttgaagcag tcgtaaacct caacagtaaa aaagttgtgg
     4981 aatggaaaca gattgtcctc gaagtgccac cctatggtaa gccgcctatt ttaattgagg
     5041 attttcaaaa atgtgagcaa atcgttaaag ccgatccgac ctggagagcc gcgatggaaa
     5101 agcgtgggct tgatgatgag caaattgaca acattcaagt agacgcattt tctgccggct
     5161 attttgatga agaggaggaa agagaacaac ggattgtcaa agggataagt ttttaccgtg
     5221 ataatttaaa ggataatggt tatgcccgtc ccattgaagg ggttgtcgct atagtagacc
     5281 ttggcaatga gaaggtattg cgtgtcattg atgacggccg caacgtccca attcctaaag
     5341 aaaaaatcaa ttacgattcg gattcttatc ctgaaaaacg caaagactta aaaccgctag
     5401 atattattca agaagaagga ccaagtttta aagtaaacgg ctgggaagtc aattggcaaa
     5461 attggtcatt ccatgttggg tttacgccgc gtgagggatt ggttttgcac cagatcaaat
     5521 acctggatga agggaaagaa aggccaatta tttaccgggc cagtgtgacg gatatgatag
     5581 tcccctatgc ggacccttcc gtgagtcatt actggaaaag cgcttttgat gcgggtgaat
     5641 atggtcttgg caaattggca aatgaactaa agctcggatg tgattgttta ggcaatattt
     5701 attattttga cgttcctgcg gcggatgatt ttggcaatgc cagattgatt aaaaatgcaa
     5761 tttgcatgca cgaagaagac gcgggcaccc tgtggaagca ttatgaaaca cgcaacagca
     5821 cctttgaggt tcgccgcgct cgtgagttgg tcgtcagctt ttttactacg ataggtaact
     5881 acgactatgg cttttactgg cgatttggac aggatggcag cctcaaactg gaaataaaac
     5941 tgactggtat tgtgcaaaca gcggcaatat ttcctgaggc tcagtatgaa tggggaggaa
     6001 aattgacacc ggagctggca gcacctacgc atcagcattt tttcaatgcc cgccttcata
     6061 tgatggttga tggtgaaaag aactcattca gtgaacacga gttttatcct atcgccatgg
     6121 gtggaaaaaa tccctatgga aatgcgtttg gcagcagcca gaaaacattt gaacatgaag
     6181 gtgaggcagc tcgctcggct tgtgcgcaaa cccagagaac ctggaagata ttcaatccca
     6241 atagcgtcaa tggcattggc acagcaccag cttacaaact ggaattgcca gaaaccccct
     6301 tactgctggc agatgaagag agttatattt ataaacgcgg gggattcgcc agcaagcagg
     6361 tgtgggtaac acaatatgac ccacaggaaa aatacgcagc aggagattat cccaatcaga
     6421 gttctggtgg ttgtggttta ccttcttaca ttaaacagaa ccgtaaaata gacaatgaca
     6481 atttggtctt gtgggtaact ctggggtcta cgcattttcc aagacctgaa gactttccgg
     6541 tcatgcctac ttcgatcatt tctgccaagc ttcagccttt tggttttttt aagaggaatc
     6601 cggctatgga tcttcctcaa ggcaaagtac aggaaaaagg ggacacctgc tgttcttcat
     6661 taacttatta atggattgga accaagcacc ttcttgagga gagggtgctt tcctgtcttt
     6721 agttttcctc ttttttatcc agcttgtgac catccagatg acgttcactg cgttcattgt
     6781 cttgtttttc ctgttgcttt tctttttttg tccttccaaa ttttatgcga ttttcagatg
     6841 cttttatttc tttttccaag cggactttag cctttctttt tttattaaga ttaatgatat
     6901 ctgccatggc actctctgtt gtaagacctt gtttatacta tagcgcataa aaccagagta
     6961 aatcatctca atacacaaat cgatcgaaat gaaatgatct gaaagtatac actttgagaa
     7021 tgggaaggac tgtcgcaaga tagtattttc agcattgttt tttatgacac tttgcaaaaa
     7081 gagcaagcat acttttaagt gctggatttt agtctataac cagagtgtat tcacagagtt
     7141 atccacagaa aatggggata agtaatcaat acatttcgcg tattaatatt taataaatct
     7201 ttacagggca aggggtttta taaaaagagc atggtgtcaa atgctaatga gattaaaaat
     7261 aaagacaagt tatccacagg aagcgagatg ctagtgaagg tatgagacgc tcattatgat
     7321 aacaaaaaaa aaaataaaaa acagtcttga ctggcacaat ttttacctga aaaagatttg
     7381 gatagactgg catcaggaat aatggcaata caaaaacagc taatcctgct ataatttttg
     7441 aacaaaaggt gagctcctgt tggcaggatt tttcacttta aatcgttcac tgacggcttc
     7501 tgagactgat ggcatagagg gtgttcagga gattttctca tgagtcgagt tttcaaagga
     7561 gcatgaaatg agtgactacc tcgataaaac cgcaagctta accccagcca ctaaaagaat
     7621 agtgtgcgac aaagctactg aatatcctca tactggctct tataatcaag tagcgactca
     7681 tggcacgtat ttatgcaggc gctgtggcct ggcgttgttt cgtggcatca gtcaatttag
     7741 ctcaggctgt ggctggccca gttttgacga tgaaatagct aacgctgtgg caaggaaacc
     7801 ggatgcggat ggtcagcgca ccgagatatt atgcgctcgt tgtgatgctc acctggggca
     7861 tgtgttcaca ggggaataca tgacgtataa gaatttacgt cattgcgtta attccgcttc
     7921 attggatttt gtcgcggata atgaagtact ggatacagaa gaagccatcc ttgcgggggg
     7981 gtgtttttgg ggagtggaat attatttaaa acgaattccc ggagtgttaa aagtcgagtc
     8041 gggctataca ggtggccatg tttcagagcc gagttatgag caagtatgca gtggtacttc
     8101 agggcattat gaggctgtgc gcgtcgtcta tgatgctgct aaaaccaatt atcatgacat
     8161 tgtaaagcga ttttttgaaa ttcacgaccc aactcaggcc gatggacaag gtcccgactt
     8221 ggggcagcaa tatttaagtg ccgtgtttta ttttaatcag gaacaacgcc aggaagcgga
     8281 gttattgatt cagttattaa agaaaaaggg gtatcaggtt gtaacccaat tattgcaagc
     8341 acaacccttt tggcctgctg aagactatca tcaagattac tattttaaac accacaaaat
     8401 tccttattgc cataagccag tcgatcgttt tggctagttg ttccggaaga tttaatctca
     8461 tcatagttgc acggatttag aaggcagaca actattggca tattcttcaa gatcatctat
     8521 tgcgcacgtg ctttcaaatt cttctgcact gcttgtcttc attttcaagt ctatggtgtt
     8581 tctccatttg agcgctttct tcatgattct ttcgttgcgc tttatatttt ctggtgtttg
     8641 gggttggtct tcaaaaaaac tacagtgctt tagaagagag tgcttgaagg tttcctcttt
     8701 agaattaatt aatggggtag aattcaggac tttatcatct gtgatgatgg ctcggctttg
     8761 tgccccgcgt aatatccgag ggggcaaact ggcacaatgc accggaaaat cagtatcaag
     8821 ttttgcaatt tttaccttgt cacttacctc agcttgttta tccataaaat cataaatatt
     8881 ttcatattta tcctgggtag acaacatgct ggcgcaatcc atagtgaagg tggcgcaccc
     8941 tgcgcgcgag tgctgtaatt taacatccgg gatgtagatt tttattgaaa gcgcctcgtt
     9001 ttcatcaagc aaatcctgga atccaacttc attcgctaat atttgagcgg tgttatagga
     9061 taattgtgcc cctaaactgt caagcaccaa aatttttaaa gtatttttac ctttttcaat
     9121 ggaatattgt agttcaatta atgaccagtg gcgcacaccg gatttctctc tatccagata
     9181 ggcaatttgt accttaaaat tattttcaaa taattttggt gaatgaatgc tgctgtaaat
     9241 aaaatcataa aatttttcac ggcgttcgta atcacagaca aaaccggtaa atggatagcc
     9301 tgattccttt gattcccgat attttttctt taaactaaag gccatttcca aatcatgagc
     9361 ggaaaatgca gggaattcgt ctagagaaaa tttatcaacg gttgtttctg atttactaaa
     9421 ggccatat
//
LOCUS       DATNSN010000130         9362 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.130, whole genome shotgun sequence.
ACCESSION   DATNSN010000130 DATNSN010000000
VERSION     DATNSN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.130"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(1..2294)
                     /locus_tag="VAH76_002416"
                     /pseudo
     CDS             complement(1..2294)
                     /locus_tag="VAH76_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945711.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
     gene            complement(2325..3383)
                     /locus_tag="VAH76_002417"
     CDS             complement(2325..3383)
                     /locus_tag="VAH76_002417"
                     /inference="COORDINATES: protein motif:HMM:NF020442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEO1387017.1"
                     /translation="MSNFQIIKTKNELDRLLQDFLKKRDEGFVFRGHADFSWELKPKL
                     FRDSEVVKSWESYGLSKKHIAENWIENKNTFITLSYIFGSQNLTKTNYKSALIPYKDQ
                     IIPLARLLWFYVDIMKYNYNLYKYINENKSYYEEEYLKQITVRSLDDWISYDTFLRLL
                     EEILYLVERRDVYTNNLLNNPEIFEDIAAYDETRPQHYDMATSALDWTYNPFIALFFA
                     CGSLANANSSRAIVIYPSTQISLFLYKQISTGDNVSVILKKPSETIVNDRAKRQEGVF
                     TWMPKARSFYLTKGNYPKLEDYRELINKEFILEEYRIEINDETATYIQEKLKERNIND
                     KTMGLEERVLSGCLDACQ"
     gene            complement(3437..6412)
                     /locus_tag="VAH76_002418"
     CDS             complement(3437..6412)
                     /locus_tag="VAH76_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEO1387018.1"
                     /translation="MTDTTKRAYSQDTIRRENVLQYHVIDQLVKKLGYVHRSTEDFDR
                     ASALDKTLVMQFIKSTQLDEWKRLQEQYSDSAETEFFKQLEKALKSRGTLDVLRQGIK
                     LIPGIKFSLCFFKPASGINKELLQLFEANILSVIDELKYSSKHDNRIDIGVFVNGIPL
                     ATIECKNQSTGTTFRHAEKQYRTDRSPAGEPLLTFKQGALVHFAMDEDNVSMTTRLAN
                     GKTRFLPFNRGHNGGAGNPDIADEFRVGYLYADQPEGKAILSRDILLDIVGRFMHLEV
                     KDGKEVMIFPRFQQLDAVLKLLRHAKQHGPGHSYLIQHSAGSGKSNTIGWTAHRMITL
                     HDEKDQPIFNTAIIVTDRIVLDRQLQNTVAQFEQTKGVVKKIDGTSRQLKEAIKQGAR
                     IIITTIHKFSTEHLKEISGQSKRRFAVLIDEAHGSQSGKHADNLSKTLTREESNNSPS
                     DIEDMIADYQRQRGPQANISYFAFTATPRNVTLERFGTKGSDGLPYPFHLYSMRQAIE
                     EGFILDVLQNYMTYKQYYKLEKAIEDDPELQGRRAHRRVARFAILHPTAIAQKVEVIV
                     EHFRRHVMQEINGKAKAMVVTQSREHALKYYFGIKKYIEDKQYVGVKALVAFSGELEI
                     DGETYTEAELNGFAETQLPEKFDKDFQLLIVAEKYQTGFDQPKLCAMYIDRKLDGLQA
                     VQTLSRLNRTTPGKINTYVLDFQNTIEDIQNAFRPFYEVTSLEEISDPNQVYELEGRI
                     KSFSIIDEDEVNRFSEVFYKGALDPHDRIALEKLVKNAVQRFEYEEDEGKREEFRQLL
                     KSYMRFYSFVAQVMSLQDTWLEKLFSYVSWLNRLLPNREVPPEIEITDDMIRLQAFRI
                     EKKEQGSASLSPGTTSPLSAIKEFGANAYTEDEERSLSEIIESFNQRHGTEFTKEDFL
                     RFEKVNQEILSDENLKDMLKNNPPDVVFGAFSQAFFESAIQLFQRDNQLQNIIMTDAE
                     ARNQAIKHFFTRALKEVRE"
     gene            complement(6409..7683)
                     /locus_tag="VAH76_002419"
     CDS             complement(6409..7683)
                     /locus_tag="VAH76_002419"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO1387019.1"
                     /translation="MNHQTFLDKFVNNYRWKSVPTKRNFRNIKQINKGLIEEHRLALT
                     LGGVIDRSLDDVEGLQSSDYSTYQIFEKDDLVFKLIDLENIKTSRVGYVPRRGIMSPA
                     YIRLTPTSELVIPRYYYWLFYAAYINNIFNGMGGGVRQNLTPTDLLEFPIPLTPKETQ
                     IEITNFLDREIDRIDQLIEKKKKLICLMRERESNAVREAIFSLINEGVQIWKLSHVCR
                     VQRGKFTHRPRNAPELYDGEVPFIQTGDVARANKFITKHKQTLSELGISVSAKFPSNT
                     LLMAIAANVGNLAITTYEVYCPDSIVGFIPTEKVESEYLYYVLRAISDDISSSSTSNA
                     QDNTNVARLGSLKIPLPSIQKQKNLIDKFKIEENLLFKTTSKISNSITKLNEFRCALI
                     SEAVTGQLDIKSWKKRGSTDERLDNIEEAMRA"
     gene            complement(7680..>9362)
                     /locus_tag="VAH76_002420"
     CDS             complement(7680..>9362)
                     /locus_tag="VAH76_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEO1387020.1"
                     /translation="NSSVRDIFLEKFLFTDQLKRLKDGGILWQVFDRFCQIDLHPDNV
                     SNMEMGYLFEDLIRRFSEISNETAGEHFTPREVIRLIVDLLLINDAEALAGSGIIRTV
                     YDPACGTGGMLALMEEAMKEYNSKIRVELYGQELNPESFGICTSDMLVTGHNPEQIAF
                     GNTLTEDAHKDKKFHYMLSNPPYGVDWKKYQDPIKQEAQEKGMDGRFGAGLPRISDGQ
                     LLFLQHMISKMRDDEVGSRIGIVMNGSPLFTGGAGSGESEIRRWMFENDWVEAIIALP
                     TDLFYNTGIQTYVWMLTNKKDKNRRGKVQLIDASSERFWQSMRKSLGSKRREISDHAR
                     SEIVKIYYEMLNGGGDWSEFSKIFDRQEFGYREIRIERPLRLNFEGSKERLELLQQEN
                     TFLKLSEIEQQELLTALNHNTLKQQFKNRDAFEKALKTALKNLSFKLTAPLKKAILTT
                     LSEKDETADICCDAKGNPEPDTDLRDHELVPLKEDWREYVEREVKPFVADAWVDENHK
                     DATDGKVGRVGYEINFNRYFYKYVPPRPVAEINEELKQLEAEIANLLKEVVA"
BASE COUNT         2731 a         1980 c         1545 g         3106 t
ORIGIN      
        1 gcccctggtg gtccttgaat agttaaataa ctattatcta aattttttac agcttgaatt
       61 atctgttgta gctttatatt tggttcatga ctcggagcta tagcaccacc acaatggttt
      121 ttaatacgtg gttttgatcg tcttaaaaaa tctaaaatag cgcacttgcc cactcgatcg
      181 ctgtcataat ccgacactaa ttgataaatg gcttgtggaa taggatctgg attaatatat
      241 tcatcgggaa ttaaagtaat taaacttggt agctcttctc tagcttgtaa tacaatcacc
      301 ccgttttcta aatcgctctc ttcactacaa aatttcacct tagcattttt gccattttcg
      361 tcttctaacc ctaataaata aaaataatca gctgcccctt taaattcttg cgtgggatca
      421 aattgatatt cataagctaa attttttgct cttggtgttg gcttaaatgg cgctctctcg
      481 gttctcttgc aaaatgccaa gcagtctaaa tcatcaaata attcttcctc gctcaaacct
      541 aatctatcaa ataaacgcca gaaaagtggc tttaactctc ggcggtgaaa ttccaacatc
      601 caagctaaat tttctgttaa tgctgcatac tctggatagt cagataatct ctgccttgcc
      661 ttggttaaca agcgatctcg taattcgatg cattgggtta tttcttcttt tggttcaggt
      721 tctactattt ctgttttacc tggataaaga atacaattag ttttccgctg ttctctcagc
      781 caagcaacca actcctgagt tgaattacaa tcatcaatgt tataatcacg aatattcttt
      841 aatatgtgag aggtttccca agtctcacct tgttcatcac gtgagtgcag gtctcgccac
      901 tgttcataaa atacaactga atcaccacca gtcccaactt cggtttcacg tttatcacgg
      961 taaagatgct cgacgttttt tatagaatac cttggttcgc ccagcaaaag gcagcccttc
     1021 acgattttat atagatcaac aaaaacttca tttcgcaata gctgatcaag ctcatactca
     1081 catataccat agcgccccat taatttacga cacgcagcta tttcataatt ggcataatga
     1141 taaatatgca tcgttggatc acgttgccat cttgcataaa cccattcaat aaactgttta
     1201 aacgcctctt tttcttgcac ctcattatgc gcccaataat ctttaaagca tcgattaccc
     1261 ttctcatcaa aataggcaca accccacaaa tattccaaac caccctcttc taaagggtat
     1321 ccctcaatat caaaaaaaac atcgagtggc gaatgtggtg gcaacaaggc aagtcctgat
     1381 ttttcattgg gtgccggtgc aataatttta tattgcgtct tttcttttcc tatactttct
     1441 ttttgtatag tagcctgcgc tttgagacgc tctaaaacat cagaattaat accaggtata
     1501 cgatttagct ttatatcaat tagttgctgc attgtttgaa taccagcttt atttaatttt
     1561 ttaatctggc tttttgtaat attagcaatc tgaaataaat gatccctttc aatcaaaagc
     1621 ttttctgcat aactcgacca gcagccccat tcctttgaat ctgcgggatc tggtatattt
     1681 cgataatcaa attgttcttg gctcaacaaa aaactttctt ttaatgagag gtaataatag
     1741 aaataatcat caacgttaaa tgcttcattt ttaccatttc cgaggacaat aataatatta
     1801 tcaggtcgcc taccttgaat ggcttctagc atttcagcat aacaacatag ctgaataata
     1861 aattcaggtt ttgctttgtg tgataatttt gcatcccaga cttcgtaatg ataatcacca
     1921 aatttacttt cgccaggcgc cttaactaga aagtctgcat atccaaaaaa ctgttgattt
     1981 tccaaataac cctgaacaat aacgtctgtg ccatttttca tggcttctaa agttaattgc
     2041 tgctttaacc cgattgtatc tgcctctatt ttcttaataa ttagtctttg agatttgaat
     2101 aattcctctt gtaatttttc atgttgaaaa cctttatttt gaagcacttc catcagctaa
     2161 tcttttggat ctttttcagg tgctttatca ggaaacccta aagagaaacg gttcatccaa
     2221 gatgcaaaag ggctgcgcat aaaaagaacc aaatcacctg gagaaaaata agaattttta
     2281 tttctgtatc tcatttttta tatattagtc cacggtaatt acaattattg acacgcatcc
     2341 aagcagccag aaagaactcg ctcctccaac cccatggttt tatcatttat attcctttcc
     2401 tttaacttct cttgaatata agtagcagtt tcgtcattaa tttcaatccg gtactcttcc
     2461 aatatgaatt ctttgtttat taattctcta tagtcttcta gctttgggta attgcctttt
     2521 gttaaataaa atgaacgtgc tttaggcatc catgtgaaaa caccctcctg cctttttgct
     2581 cgatcgttca caattgtttc gctaggtttt tttaatatca ctgaaacatt atcaccggtg
     2641 cttatttgct tatataaaaa taaagagatc tgcgtagaag gataaataac tattgctctt
     2701 gatgaatttg cattagcaag acttccacac gcaaaaaata aagctataaa agggttatat
     2761 gtccaatcta acgcagaagt tgccatatcg taatgctgcg ggcgagtttc atcgtatgca
     2821 gcaatatctt caaaaatttc tgggttattc aatagattgt ttgtataaac atctctacgc
     2881 tcaacaagat atagaatttc ttcaagcaat ctcagaaaag tatcataact tatccaatca
     2941 tccaaactac gaactgtgat ctgttttaaa tactcctcct cataataact cttattctcg
     3001 ttaatatatt tatacaagtt gtagttatat ttcataatat ccacataaaa ccaaagtaat
     3061 ctcgctaatg ggattatttg atctttataa gggatcaacg cacttttata attagtttta
     3121 gttaaattct gtgaaccaaa aatatacgat aatgtaataa aagtattttt attttcgatc
     3181 caattttcag caatatgctt tttcgataag ccataacttt cccatgactt tactacctcg
     3241 ctatcacgga ataattttgg ttttaattcc cagctaaaat cagcatgccc acgaaaaaca
     3301 aaaccttcat ctcttttctt tagaaaatct tgtaacaacc gatcgagttc attttttgtt
     3361 tttatgattt gaaaattact caattttaca cattcctcgg ttaataaatt acttggcgac
     3421 attccaatgt aagctattat tctctaacct cctttaacgc ccgggtaaag aaatgtttaa
     3481 tcgcctgatt tcgtgcctca gcgtctgtca tgattatatt ttgcaattga ttatcacgct
     3541 gaaataattg aatcgcactt tcaaaaaaag cctgagaaaa agcaccgaaa accacatccg
     3601 gtggattatt cttcagcata tcctttaagt tttcatcact tagtatttct tgattaactt
     3661 tctcaaaacg caaaaagtct tctttagtaa attcagtgcc atgacgctga ttgaaagact
     3721 caataatttc agataatgaa cgctcctcat cttccgtgta agcattagca ccaaactctt
     3781 tgatagcaga tagaggagaa gttgtgccag gagataacga agctgaaccc tgttcttttt
     3841 tctctattct gaatgcctgt aaacgaatca tatcgtctgt aatctcaatt tcaggtggca
     3901 cttctcggtt aggcagtaat ctattaagcc atgatacata ggaaaaaagt ttttccagcc
     3961 aagtgtcttg caaactcatc acctgcgcga caaatgaata aaaacgcatg tagctcttca
     4021 gcaattgtcg aaattcttca cgctttcctt cgtcttcttc atattcaaaa cgttggacag
     4081 cgttcttaac caacttttct agcgcgattc tatcgtgggg atcaagcgct cctttataga
     4141 aaacttctga gaaacggtta acttcatctt cgtcgataat agaaaaagac ttaatgcgac
     4201 cctccaactc ataaacttga ttagggtcag atatctcctc cagcgacgta acttcataaa
     4261 atggtcgaaa tgcattttga atatcttcta ttgtattctg gaaatcgagc acgtatgtat
     4321 taatctttcc tggggtagta cgatttagcc ttgatagggt ttgtactgct tgcaaaccat
     4381 ccagttttct atcgatatac atggcacata actttggctg atcaaaaccg gtttgatatt
     4441 tctctgccac aatcaataat tgaaaatctt tatcaaattt ttctggcaat tgggtttcag
     4501 caaagccgtt taattctgct tcagtgtagg tttcaccatc tatttctagc tcaccagaaa
     4561 aagccaccaa ggctttcaca cctacatact gtttgtcctc aatatacttt tttataccaa
     4621 aataatattt aagtgcatgt tcgcgacttt gagtgacaac cattgcttta gctttaccat
     4681 ttatttcttg cataacatgg cgacggaaat gctcaacaat aacctccact ttctgagcaa
     4741 ttgccgttgg gtgtaatatc gcaaatctag caacacggcg gtgcgctctt cgcccttgca
     4801 attcaggatc atcttcaatg gctttttcca gcttatagta ttgtttatag gtcatgtaat
     4861 tctgcaacac gtcaagaata aaaccctctt caatagcttg tcgcatagaa taaagatgga
     4921 acggatatgg caaaccgtct gatccttttg taccaaaacg ctccaatgtg acattacgtg
     4981 gtgtagctgt gaaagcgaaa taactgatat tagcctgtgg cccacgctgg cgctgataat
     5041 cagcaatcat atcttctata tcagacggac tattattgct ttcttcacgt gtcaaagttt
     5101 ttgagaggtt atcagcgtgc ttacccgact gagaaccatg cgcttcatct attagtacag
     5161 caaatcggcg cttgctttga ccggaaattt ccttcaggtg ctcagtcgaa aatttatgga
     5221 tggtagtaat gataatacgc gcaccttgct taatggcttc tttcaactgt cgcgaggtgc
     5281 catcaatctt tttcaccacg cccttagttt gctcaaactg agccacagta ttttgtagtt
     5341 ggcgatctaa cacaatacga tcagtcacaa taatggctgt gttaaaaatt ggttggtctt
     5401 tttcatcatg tagagtaatc atacggtgag ctgtccaacc aatagtatta gatttacctg
     5461 aacctgctga atgctgaatt aaatagctat gacctgggcc atgttgtttt gcatgtctta
     5521 ataacttcaa tacagcatct aactgctgaa agcgcggaaa tatcataacc tctttaccat
     5581 cttttacttc caggtgcata aatcgtccaa cgatatcaag cagaatatct cgcgagagga
     5641 tggcttttcc ttctggttgg tcagcataaa gataaccaac acgaaattca tcggcgatat
     5701 cagggtttcc tgcaccacca ttatggcctc ggttaaatgg caaaaatcga gttttaccgt
     5761 ttgctaatcg tgtagtcatg gaaacattgt cttcgtccat tgcgaaatgt accaatgcgc
     5821 cttgtttaaa ggttagtaat ggctcacctg ctggggaacg atcggtacga tattgttttt
     5881 cagcatgacg gaacgtcgta cctgttgatt ggtttttaca ttcaatagtc gccaatggaa
     5941 tgccattaac aaacacgcca atatcaatgc ggttatcatg tttactgcta tatttcagct
     6001 cgtcaatcac cgaaagaata ttggcctcga agagctgtaa tagttcttta ttaatacctg
     6061 aagccggttt gaagaagcaa agagagaatt taatgccagg aataagtttg ataccctggc
     6121 gcagcacatc cagcgttcca cgagatttca acgctttttc caattgtttg aaaaattcgg
     6181 tttcggcgct atcagaatat tgctcttgca atcgtttcca ctcatcaagc tgtgttgatt
     6241 taatgaattg catcactaaa gttttgtcga gtgctgaagc acgatcaaaa tcttctgtgc
     6301 tacgatggac ataacctagt tttttaacca gctgatcgat cacatgatat tgcagcacat
     6361 tttctcgacg gatagtgtcc tgagagtagg ctcgtttagt tgtatccgtc atgcccgcat
     6421 tgcctcctcg atattatcga ggcgctcatc ggtgctacct ctttttttcc atgatttaat
     6481 gtctaattga cctgtaactg cttcagaaat taaagcgcat ctaaattcat tcagctttgt
     6541 aattgaatta gaaattttac tagtcgtttt gaatagtaga ttctcttcta ttttgaattt
     6601 atctatcaga tttttctgct tttgaatact tggcaaggga atttttaaac ttcctaagcg
     6661 agcaacattg gtattatctt gagcattact tgttgaactg gaagagatat catcgcttat
     6721 tgccctaagt acataatata agtactctga ttcaaccttt tcagtaggaa taaaaccaac
     6781 aatactgtct ggacagtata cttcataagt tgtgatagcc aaattaccca catttgccgc
     6841 aatagccata agcaatgtgt ttgaggggaa cttagcgcta actgatatac ctaactctga
     6901 tagagtttgc ttatgtttgg ttataaactt attggctcta gcaacatctc ctgtttgaat
     6961 gaaaggcacc tctccatcat aaagttctgg agcatttcta ggtctatgcg tgaattttcc
     7021 tctttgtact ctgcatacat gagataattt ccaaatttga accccttcat ttataaggga
     7081 aaagatagct tccctaacag catttgactc tctctccctc attaaacaaa taagtttttt
     7141 cttcttctca atcaactgat caatacgatc gatttcacga tccaaaaaat ttgttatttc
     7201 tatttgagtt tcttttgggg taagaggtat aggaaattct aataagtctg ttggagttaa
     7261 attctgccga acacctccac ccataccatt aaaaatatta tttatgtatg cagcataaaa
     7321 aagccagtag tagtatcgtg gtatcactag ttcggacgta ggtgttaatc gaatatatgc
     7381 gggactcatt atacctcgtc gtggaacgta tccaactcgg ctagttttga tattttccaa
     7441 atcaattaat ttaaaaacaa gatcgtcctt ctcaaaaatt tggtaggtcg aataatcgga
     7501 cgactgtaat ccctctacat catctaggga tctatctatt acacctccaa gcgtaagtgc
     7561 aaggcgatgt tcttctatca aacccttgtt aatttgtttt atatttctaa agtttcgttt
     7621 tgtaggaaca gatttccatc gataattatt tacaaatttg tccaagaaag tctgatggtt
     7681 catgctacta cctctttcag caagttagca atttccgctt ccaattgttt aagctcttca
     7741 tttatctcgg ctacaggacg cggtggaaca tatttataga aataacgatt aaaattaatt
     7801 tcatacccca cacgacctac cttgccatcg gtggcatcct tgtgattttc atctacccaa
     7861 gcatcggcaa caaaaggttt tacttctcgt tcaacatatt ctcgccaatc ttcttttaga
     7921 ggtactaatt catgatcgcg taaatctgta tcaggttcag gattgccttt agcgtcacaa
     7981 caaatatcag cagtttcgtc tttctctgaa agcgttgtaa gaatcgcttt ttttaatggg
     8041 gcagttaact tgaaacttaa attttttaat gctgttttaa gtgctttctc aaatgcatca
     8101 cgatttttaa attgctgttt taatgtgttg tgattaagtg cggttaataa ttcttgttgc
     8161 tctatttcgg aaagttttag aaacgtattt tcttgttgaa gcaattctaa gcgctctttg
     8221 cttccttcaa aatttagcct caaaggccgc tcaatgcgta tttctcgata accaaactct
     8281 tgcctatcaa aaattttaga aaattcgctc caatcaccac caccattgag catttcataa
     8341 taaattttaa caatttcact acgcgcatga tcagaaatct ctcgacgttt gctaccaaga
     8401 ctttttcgca tggattgcca aaatcgttcg ctacttgcat caatgagctg cacctttccc
     8461 cgacggttct tatccttctt attggtaagc atccaaacat aggtttgaat accggtgttg
     8521 taaaacaaat cggtaggtaa tgcgataatc gcctctaccc aatcattttc aaacatccag
     8581 cggcgaattt cactttcacc agagcctgct ccacctgtaa aaagaggaga gccattcatc
     8641 actataccaa tgcgtgagcc tacttcatca tctcgcattt ttgatatcat gtgctgcagg
     8701 aaaagcaact gtccatcaga tatacgcggt agcccagcac caaaacgacc atccatccct
     8761 ttttcttgcg cttcttgctt aatagggtct tggtactttt tccaatctac cccatacgga
     8821 ggattggaaa gcatataatg aaatttttta tccttgtgag catcttctgt taacgtgtta
     8881 ccgaaagcaa tttgctctgg gttatgacct gtcaccagca tgtcagacgt gcagataccg
     8941 aaggattctg ggtttagttc ctgcccgtag agctcgacac gaattttaga gttatattcc
     9001 ttcattgcct cttccataag tgcaagcata ccgcctgttc cacaggcagg atcataaact
     9061 gtgcgaatga ttcctgaacc agccaatgct tcggcatcat taatcaacaa aagatccaca
     9121 atcaaacgaa tgacttcacg aggtgtgaaa tgttcccctg ccgtctcatt cgaaatttca
     9181 gaaaaacggc gaatcaaatc ttcaaagaga taccccatct ccatgttgga aacattatca
     9241 ggatgtaaat ctatctggca gaaacgatcg aatacttgcc ataaaattcc accatccttt
     9301 aggcgcttaa gctgatcagt aaagagaaat ttttcaagaa aaatatcgcg aacgctgcta
     9361 tt
//
LOCUS       DATNSN010000131         9359 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.131, whole genome shotgun sequence.
ACCESSION   DATNSN010000131 DATNSN010000000
VERSION     DATNSN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.131"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            536..1678
                     /locus_tag="VAH76_002421"
     CDS             536..1678
                     /locus_tag="VAH76_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387021.1"
                     /translation="MLSKYDKLIYINLAQDQVTANIDTLIIHESPTILVKIPDGSDLK
                     TLVDPDFFPGPITNRTKIYFSAHGHEELDDMVLDRQKENPKIYKLDDVAAYFGKLLTN
                     PDFKNPDIKPRLTLVMSVCEGLGFAKNLQKKLFDDYGLLVDVIANKNVIHEQFVKPHH
                     ETTAYLTHRETSIKGVGREHKRPHSKVLLTIDKEGTQQEIDAYELKWIEKVLNTIHQQ
                     VATFGKWADFEKPENIKIQEGLNAFCHDVDALISLFINQDINLTANNLLALLLSCQKT
                     TADPLYKQAMNYEMFQIMTKNLINEGLRYINPKIEMQEYSHALEELQSQKDKMIRDEV
                     APNIFRAKKAIFSALLAEASEYGIDSVLEQLEKAKQELSQGKMNPS"
     gene            complement(1846..3078)
                     /locus_tag="VAH76_002422"
     CDS             complement(1846..3078)
                     /locus_tag="VAH76_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEO1387022.1"
                     /translation="MNRPVVIVDPLSSGIELAPAFKARGIPAIAVTLKPLDWIGFGAN
                     MQTSDFIEIIPDQPNLVEVLAKYDPIAIIPGTEEGVPLAEALAITLTPQFANDPEKSQ
                     NRLHKALMQKALQEAGVPALKTLNTASESEVEAWIRTNGLIDSPLIIKPPVSAGSDKV
                     FHIPARGDWKKEFNRVLSEPSKITGKNNETVVVQEQAIGTEFAVGTVSANGKHYLAHL
                     IQYNKTSFNDRKTVYNYVEFVPYSQERYGELFEYTQKALDALGVRWGAAHNEIMLTKD
                     GPRLIETGARMCGGPVVGFAREATGSSQADKLVEIYVDGGVSTEEYVFKKTVVPVFLK
                     SPAKGKISNVEAFAELSKLPTFLNEHIWFKNGDLVPQTVDYLTSIGIIGLAGDRKSIL
                     LDYEKIRNMESKLVIQTF"
     gene            complement(3075..4223)
                     /locus_tag="VAH76_002423"
     CDS             complement(3075..4223)
                     /locus_tag="VAH76_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387023.1"
                     /translation="MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGV
                     SLFSLTYLFSAPLFSLIFVNQPVKRMIQLAFTVFMLGNLITLLSENIMLFLIGRSLAG
                     VGSGIFTPLCISIAVHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSSFHWQLSI
                     ASLIILSLLALVGFSMQNIDIRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGL
                     YSYVTLIQSGSPNSLSMTLLSWGLGGFMGSSLIGVCIDRTGKPRVIMALILVGLMFAL
                     IAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGTILAAINSSALGLGA
                     ALGTLLGGLLISSGFRGIYLPFSAATLLFFVLITQLIVINTSHKVNNT"
     gene            complement(4220..5662)
                     /locus_tag="VAH76_002424"
     CDS             complement(4220..5662)
                     /locus_tag="VAH76_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEO1387024.1"
                     /translation="MSAQVADVVIVGAGPVGLMCAYLGQRCGIHTVIVDKSDGPLEVG
                     RADALNARTLQLLELVDLFDELYPLGKICNTSSVWADGQFISRQSSWWEELEGCLHKH
                     FLMLGQSYVEKLLDEKLKETAAAVKRSTAIVNIEINEAGCLTTLANGECIQSRYVIGA
                     DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIDTDFPKVPEIIVFQAETSDVAWIPREG
                     EIDRFYVRMDTKDFTLQEAMDKINHAVRPHSLNFKDIVWFSQFSVKESVAESFFIQDR
                     IFLAGDACHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKELLHSYEAERKPVAH
                     GVIETSGELVRSTKYSLNGTHAQDYVRIVQKRAGYITGMGIRYGETGLCGSRLFDFEI
                     FNGIAKTRLYSLLDYMKFTLLIFGDCEVELEPPEFVKVIPIYPTQCQENFWTDNTHYA
                     NQAILVRPDSYIQYSVPLDKMESLFELAQL"
     gene            complement(5659..6495)
                     /locus_tag="VAH76_002425"
     CDS             complement(5659..6495)
                     /locus_tag="VAH76_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEO1387025.1"
                     /translation="MNYKVTSLKPFGVLLEPVSEKTKVTDVDIENLRHLFAKNQLVVL
                     RGFCAFQNAEDFSNYCELWGEVSLWPFGKVLELIEQEDPEDHIFDHSYMPLHWDGMYR
                     PQVPEYQIFHCVKAPLPGQGGRTTFSNTILALQFASSEIKELWNKVCGTYQRKMEFYN
                     SKTVSPIITKHPQKDFSVIRYNEPPSADKGHFVNPPDIEFTGIDQEELDFFHRSLNKA
                     LYSADNFYAHEWQNGDIVIADNFSLLHGREGFVSRSPRHIQRVHVLSNPPFDNPGLES
                     YQ"
     gene            complement(6485..7534)
                     /locus_tag="VAH76_002426"
     CDS             complement(6485..7534)
                     /locus_tag="VAH76_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocyanide synthase family protein"
                     /protein_id="HEO1387026.1"
                     /translation="MKNTAFLITKNRYTKDYRLKKSPDSDSAMGVAKRILAEVMIFRR
                     VPETISLCGTGCQKCASPHLPKIISAVNKNEPVTFILPAFPGKSPNPEKVLGHLPDHA
                     ERLSLNFLGKLCQRIKNFYTPGIKIILCSDGRVFSDVVGMNESDVTAYQLELDRLIKE
                     MSLADLSTFNLDYFYKDLHFVQMREELMKSYGQSLDFLKQKIRNGAKPSASPDEQEAN
                     RMYSGITRFLFEDAMHAGQTKSRTAIQKESRFKAYEVIRRSNAWSALIAERFPKAVRL
                     SIHPQTCGSKKLGIRLIGNESWMTPWHGVAVESNKGYVLLRRSEAEALGAKLICSSDG
                     RHSHYQLMAEVSYEL"
     gene            7645..8505
                     /locus_tag="VAH76_002427"
     CDS             7645..8505
                     /locus_tag="VAH76_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387027.1"
                     /translation="MSLLLDDIKYFIAASDTLNMTRASEIIGISQPALSYSIKRLENK
                     LGGLLFIRLKNGIQLTKLGEEFKRRSHRLLYEWEQVQNIANPESGFVQGNYTIALHPS
                     VALYTLQYFMPKLQVNYPGLGINFIHGHSREMTEKVISWEADFGIVVNPIQHPDLVIL
                     KLSTDEVTVFYANDAQNKLIYDRKLAQSQYILKKIGKKITFNGVINSTSLEVVAKLTA
                     LGLGYGILPTRVTYPYKHLKKVLNAPVFRDEICLVYRPEKHNNPVSKKIIQIIRSSIS
                     NDNDKENYFD"
     gene            complement(8630..9328)
                     /locus_tag="VAH76_002428"
     CDS             complement(8630..9328)
                     /locus_tag="VAH76_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0172 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387028.1"
                     /translation="MTMLYIKNLRYNEVAVCRALFEAAEKTEEGIKTKRYGMQLTADM
                     PGLWSPLSNYDIENHLRMARIHRNGRMREIGYLTVNLVFQFDSLDVTEYETKYGISAK
                     DVLEKAGFTCSQDAAGYSEPVSTSTRLLGQESKPEKPTQTSVVNTSFFSLKSVDTDKR
                     FTKSTKPGILYEARSYGKIEDDTLKSYGFTDGQWERQKEGIENIVIIKKLTQSISPGP
                     AVLDEAVDNIPTLS"
BASE COUNT         2967 a         1956 c         1734 g         2702 t
ORIGIN      
        1 aaattaataa tataagtata gacagcgaga ggttgacatt gaattagtga gcgaaatttt
       61 agataaaatt ctatgattag gatatctatc gaacaatatc gaaatattag caaactacac
      121 acttgctact aaaaagtctt acgataagtt tggatttaga cagaccgaac ttccttactc
      181 catcgaagga gatatagagt tagtctcgat gctattgaca aatcagagtc taatacagaa
      241 ttattataaa aaacgaaatt ttattaaaaa aattactttc tttggtaatc ttgatcagct
      301 tgatgagaaa gcagaaaacc attcagttgg atatactcat tcattggctt gttttttcat
      361 cagcctctaa atacccccag catagattga ccgcttctaa ttggataagc aaaatctcaa
      421 catgcgatga agttattttc ttaaattact ctgtttgttt actccattta tggtgaattt
      481 ttaatcaaag attctatact ttaagaatat cctcaaacca aaatggagct ctatcatgct
      541 ttccaaatat gataaactca tttacatcaa tttggctcaa gatcaagtaa cggcaaatat
      601 tgatacactt ataattcatg aaagccccac aatactcgtg aaaataccgg atggaagcga
      661 cctaaaaacc ttggttgacc cagatttctt tcctggaccg ataacaaatc gcaccaaaat
      721 atatttttca gctcatggac acgaagagct agatgacatg gtccttgata gacaaaaaga
      781 aaatccaaag atatacaaat tagacgatgt tgctgcctat tttggcaaat tactaacgaa
      841 tccagatttt aaaaatcctg atataaaacc tcggttgaca ctagtgatga gtgtatgtga
      901 aggattaggt tttgccaaaa acttgcaaaa aaaattattt gatgactatg gattacttgt
      961 cgatgtgatc gctaataaaa atgtaataca tgagcaattt gtaaaacctc accatgaaac
     1021 aacggcctat ctcactcata gggagacatc cataaaagga gtgggtagag aacacaaaag
     1081 accacatagc aaagtattat tgactattga taaggagggg acacaacagg aaattgatgc
     1141 ttatgaatta aaatggatag aaaaagttct taatacgatt catcaacaag tagcaacttt
     1201 tggcaaatgg gctgattttg aaaaaccaga aaatatcaag atacaagaag gcctcaatgc
     1261 tttttgccat gatgtcgacg ccctcatttc gttattcatc aatcaagaca ttaatttgac
     1321 tgctaataac ctcttggcat tgctgttaag ttgtcagaaa accaccgctg atccattgta
     1381 taaacaagca atgaactatg aaatgtttca aatcatgaca aaaaatctca ttaatgaagg
     1441 gttaagatat attaatccga aaattgagat gcaagaatat tcacatgctc ttgaggaact
     1501 tcaatcacaa aaggacaaaa tgattcgaga tgaggttgcc cctaatatat ttcgggcaaa
     1561 aaaggcaatt ttttcggcat tacttgccga agcttcagaa tatggcattg attcagtctt
     1621 ggagcaactt gaaaaggcaa aacaagaact atcccaagga aagatgaacc cgtcttaatt
     1681 aataccatga ctagtccctg tttcgcttat ttattctttt agtgggacgc gaaaataaag
     1741 cccgctaaag ctggtcttag tataactgca aaaaatcgtt aaagaccata aaagatagga
     1801 aaaataaagg aaaaatattt ccatagaaat taagcaatag aaaccctaaa atgtttggat
     1861 aaccaatttc gactccatat ttcgaatttt ttcgtaatcc aataaaatgg acttgcggtc
     1921 tccagccaac ccaataattc caatactcgt taaataatca acggtttgtg gaacaaggtc
     1981 accattttta aaccaaatat gttcattaag aaaagtcggt aatttcgata gttcggcgaa
     2041 ggcttcaaca ttcgaaattt tgcctttagc gggtgacttc aaaaaaacag gcacaaccgt
     2101 ttttttaaat acatattctt ctgttgaaac acctccatcg acatagattt ccactagttt
     2161 atcggcttga ctgctgccag tcgcttctcg agcaaagcca accactgggc cgccacacat
     2221 tctggcgcct gtttctatga gacgagggcc atctttagtg agcatgattt cattgtgagc
     2281 agctccccag cggactccca atgcgtccaa tgctttttgc gtatactcaa ataattcacc
     2341 atatctttct tggctataag gcacaaattc cacataattg taaaccgttt tgcgatcatt
     2401 aaaggatgtt ttattatatt ggattaaatg cgctaaatag tgttttccat tggcactcac
     2461 cgtacctaca gcaaactctg ttcctatagc ctgctcttga acaacgacgg tttcattgtt
     2521 tttcccggta attttagatg gctccgataa aacccggttg aactcttttt tccaatcccc
     2581 tctggctgga atatgaaaga ccttatcact gccagcagat actgggggtt taataataag
     2641 tggggaatca attaatccgt ttgtcctgat ccaggcttca acctcacttt cagatgccgt
     2701 attgagcgtc ttaagggcag gaactccagc ttcctgcagc gctttttgca tcagagcctt
     2761 gtgtaatcta ttctgtgatt tttcaggatc attggcaaat tgaggcgtca aggttatcgc
     2821 caacgcttcc gcgagaggga ctccttcctc agtgcctggg ataatggcaa tagggtcgta
     2881 ttttgcaagt acctccacaa gatttggttg gtctggaata atctcgataa aatccgaggt
     2941 ttgcatgttt gctccaaatc caatccaatc cagaggttta agcgtgactg caatagcagg
     3001 tatcccccga gccttaaagg caggagccaa ctctattcct gatgataatg gatcgacaat
     3061 gacaacgggc ctgttcatgt gttatttacc ttatgtgaag tgttaattac tatcagctga
     3121 gttatcaata caaaaaacaa taaagtggca gcagaaaaag gaagatagat tcccctgaat
     3181 ccagatgaaa tcaataagcc gccaagcaga gttcccaaag ccgcccccaa gcccaaagcc
     3241 gatgaattga tagcggcaag gatagttccc tgattttcat gtaattcata taaaatgtgt
     3301 tgctggggag tcactatagc ccatccgcaa gcaccccaca taaaaaaagg gattaatccc
     3361 aggtaaggca gattcctggt gaatggtatg gcaatcagag caaacatgag gccaaccaaa
     3421 attaaggcca taataacccg tggtttacct gttcgatcga tacacactcc aatcagtgag
     3481 cttcccataa atcctcccag tccccaactc aatagcgtca tactgagcga attaggggat
     3541 cctgattgaa tcagggtgac atacgagtat agtcccaaac ttgccaaggc ggtaaaacaa
     3601 gtaattccaa ttaccgatag cgtttttggc tcgaccagaa gacgaagcct gcctccaaat
     3661 ggcgaaggtt tgggtagtct tatgtcaatg ttttgcattg aaaaaccaac caatgcaagc
     3721 aaacttaaaa taataagact ggcaatcgat aactgccaat gaaacgagga ggataagtaa
     3781 agtccggcag gaacgccaaa cactacaccc gcactgttag cactccagat aaaacttaaa
     3841 attcgtcctt tggcagatgg gctggcaaaa tgaacggcaa tgctgataca taacggcgta
     3901 aaaatcccgc ttcctactcc cgccagagat cttccaatta aaaacagcat gatattttca
     3961 gaaagtagag ttattaaatt gccaagcatg aagacagtaa acgcaagctg aatcatacgc
     4021 tttaccggct gattaacaaa aatcagagaa aaaagtggcg ctgaaaaaag ataagtcaaa
     4081 ctgaatagac tcactccttg cccagttacc gcaaccggct gcccaagcgt ttcgcctatt
     4141 tgaggcagca gaccagcaat tagaaatgta ttgcaaccta ctgtaaacat tcctattgct
     4201 aaaaaaatca ggcttttctt cataactgtg ccaattcaaa taaggattcc attttatcca
     4261 gcggcacaga gtattggatg taggagtcag gtcttactaa aatggcctgg tttgcatagt
     4321 gcgtgttatc ggtccaaaaa ttttcctgac attgggttgg atatattgga atgactttga
     4381 caaactccgg aggctctaac tcgacttcac aatcgccaaa gataagtaaa gtgaatttca
     4441 tataatccag aagagagtaa agccgtgtct tggcaatacc attaaaaatt tcaaaatcaa
     4501 agagtcgaga accacacaaa cctgtttctc cataacgaat ccccatcccc gtgatataac
     4561 ccgcgcgctt ttgcacaatc ctgacatagt cttgggcatg ggttccatta agcgaatact
     4621 tggtcgagcg cacaagctca ccggaagtct ctatcacacc gtgagctact ggtttacgct
     4681 cagcttcata actgtgtaaa agttctttgg gagcgccgaa atgcatgacc atgtgcaatt
     4741 tccacatgag attaaatgca tctgcaagac cagtgttaag accttgccca ccgtttaccg
     4801 aatgaatatg gcaagcgtca ccggcaagaa aaatgcgatc ctgaatgaag aaactctcag
     4861 cgacagattc tttaactgaa aattgtgaaa accagacaat gtccttaaaa tttaaggaat
     4921 ggggccgcac ggcatgatta attttgtcca ttgcttcctg caaggtaaag tctttagtat
     4981 ccattcttac gtaaaacctg tcgatttccc cttcccttgg aatccaggcc acatctgaag
     5041 tttctgcctg aaacacgatg atttcaggaa cttttggaaa gtcggtgtca atcatgccat
     5101 cgatgaccgc ccatataatt tgtggccgta tgatctcaaa aggtattgca aagtggttgc
     5161 gaacaaagga acgagcccca tcggctccga tgacatagcg tgactgaata cactctccat
     5221 tggcaagagt cgtaagacat cctgcttcat taatctcaat gttgacaatc gctgttgaac
     5281 gttttactgc tgccgcagtt tccttcagct tctcatctaa caatttttct acataggatt
     5341 gaccaagcat aaggaaatgt ttatgcaaac aaccctccaa ttcctcccac catgacgatt
     5401 gtcgggaaat gaattgtcca tctgcccaaa cagaactggt attgcagatc tttcctaaag
     5461 gataaagttc atcaaataaa tcaactaatt cgagaagttg taaagtacgc gcgttaaggg
     5521 catccgctct accaacctct aaaggaccat cagacttatc gacaatcacg gtatggatac
     5581 cacaacgttg tcccaaatag gcacacatga gccctactgg acccgcaccg acaattacaa
     5641 cgtctgcaac ttgtgcgctc attgataaga ctccagaccc gggttatcaa aaggcggatt
     5701 actcaaaaca tgaacgcgtt ggatatggcg aggcgatctg gagacaaacc cttctctgcc
     5761 atgtaataaa gaaaaattat ctgcaatgac gatatctccg ttttgccact catgagcata
     5821 gaaattatcc gctgaataaa gtgctttatt taaacttcgg tggaaaaaat ccaactcttc
     5881 ctgatcaata ccagtaaatt caatatcggg tggatttaca aaatgacctt tatctgcaga
     5941 gggaggttca ttgtagcgaa tgactgaaaa atccttttga ggatgcttcg tgataattgg
     6001 tgataccgtc ttgctgttat aaaactccat tttcctttgg taggtaccac acaccttatt
     6061 ccataattct ttgatttctg aagaggcaaa ctgtaacgct aaaattgtgt ttgaaaaagt
     6121 ggtccttcct ccttgtccag gcaaaggcgc tttcacacaa tgaaaaatct gatattcagg
     6181 cacctggggt cgatacatac catcccaatg caagggcatg taactatgat caaaaatatg
     6241 atcttccgga tcctcttgct caattaactc aagtaccttg ccaaatggcc aaaggctgac
     6301 ttctccccac aactcacaat aattagaaaa atcctccgca ttttgaaagg cgcaaaagcc
     6361 tcttaataca acgagttgat tctttgcaaa taaatggcgc aagttctcta tatcgacatc
     6421 agtcaccttc gttttttcac tcacaggctc caagagtact ccaaatggtt ttagagaagt
     6481 cactttataa ttcatatgac acctccgcca ttaattgata atgactgtgc cgaccatcag
     6541 aggaacaaat taattttgca cccaaagcct cagcttccga gcgcctcagc aagacatacc
     6601 ccttattgct ttcaacagca acaccatgcc aaggagtcat ccagctttca ttccctatca
     6661 accgaatccc taattttttt gatccgcaag tttgtggatg aatagacagt cgaactgcct
     6721 tgggaaagcg ttcggcaatg agcgcactcc aggcgttgct tcttcttatg acctcatagg
     6781 ctttaaaacg agattctttt tggatagcag tacgactttt ggtttgacct gcatgcatgg
     6841 catcttcaaa caaaaagcgc gtaattccac tatacatgcg attggcttct tgttcatcgg
     6901 ggcttgctga gggttttgca ccattacgga tcttttgttt aaggaagtcc agtgactgtc
     6961 catagctttt catgagttcc tcacgcattt ggacaaaatg aagatcttta taaaaataat
     7021 caagattaaa agtggacaga tctgcaaggg acatttcttt tataagtctg tccagctcaa
     7081 gctggtaggc tgtgacatca ctttcattca ttccaaccac atcgctaaaa acccttccat
     7141 cggaacacag aataatttta atccccggtg tataaaaatt ttttattcgc tgacaaagtt
     7201 ttcccaaaaa attaagagaa aggcgttcag catgatctgg cagatggccc agaacttttt
     7261 cagggttggg agattttccc ggaaaggcag gcaatataaa agtaaccggt tcattcttat
     7321 taactgccga gatgattttt ggcaaatggg gtgaagcgca cttttggcat ccggtgccgc
     7381 ataaactaat ggtttccggg acacggcgaa aaatcattac ttcagctaat attcttttgg
     7441 ctactcccat agcagagtca ctatcgggtg attttttcag tcgataatct ttagtgtaac
     7501 gattctttgt aattaaaaac gctgtattct tcattttcct tccctcctga aaaagagagt
     7561 acagatcttc gacaaataat taaaatgatt tattttaatt gttgtgataa attttactta
     7621 tacctaaaat ggagttgatg agttatgtct ttattgttgg atgatataaa gtatttcata
     7681 gcggccagcg atacgctcaa tatgacaaga gcttctgaaa ttattggaat atctcaacct
     7741 gcgcttagtt attctataaa gagattggag aacaaacttg gtggattact atttataagg
     7801 cttaaaaatg gaatccagct tacaaaactt ggagaggagt ttaagaggcg ctctcaccgt
     7861 ctattatatg aatgggagca ggtgcaaaac atagcaaacc ctgaatcagg ttttgttcag
     7921 ggaaactata ctatagccct tcacccttcc gttgcgcttt acactctcca gtactttatg
     7981 ccaaaactgc aagtgaacta tcctgggctt ggtattaatt tcattcatgg tcattcaaga
     8041 gagatgactg aaaaagtgat aagttgggaa gccgattttg gtattgttgt gaaccccata
     8101 caacaccctg atctcgttat attaaagctg tctacagatg aagtgacagt cttttatgcc
     8161 aatgatgcac aaaataaact gatttatgat agaaaacttg cccaatcaca atacatcctt
     8221 aaaaaaattg gcaaaaaaat aactttcaat ggcgtaatta attctaccag tcttgaagtt
     8281 gtagccaaac tcacagcctt aggtcttggg tatggcattc tacctaccag ggtcacctat
     8341 ccctacaaac acctcaaaaa agtactcaat gcgcctgtgt ttagagatga gatttgtctt
     8401 gtctaccgac ctgaaaaaca taataatcct gtcagtaaaa aaatcattca aattatcaga
     8461 tcatctataa gcaatgataa tgataaagaa aattattttg attaattcgg ttattacaat
     8521 gagttttgct tgttttttga attaacagaa ttgattagga aaatataaaa caccgaaatt
     8581 gataatagtc cattattttt caatttcagt attttaaaat tgatgctctt taagatagtg
     8641 ttggaatatt atcaacagct tcatccaaaa cggctggacc tggagaaata ctctgggtga
     8701 gttttttaat aatcactata ttctcgatgc cttctttctg cctttcccat tgtccgtcgg
     8761 taaaaccata gcttttcaaa gtatcatctt ctattttacc gtaggatctt gcctcgtata
     8821 atattcctgg ttttgtcgac ttggtaaatc ttttatctgt atccacactc tttagagaaa
     8881 aaaacgaggt atttacaaca cttgtttggg ttggtttttc gggtttgctt tcttggccta
     8941 ataaccttgt tgaagttgaa acaggctcag aataacccgc tgcatcttga ctgcatgtaa
     9001 agccggcttt ttctaaaacg tcttttgctg aaatgccata ttttgtttca tactctgtaa
     9061 catcgaggct atcaaattga aatacaagat taactgtcaa atatccaatt tccctcattc
     9121 ttccattgcg atgaattctt gccattctga gatgattctc aatgtcataa ttagaaagag
     9181 gagaccataa accaggcata tcagccgtca gttgcatgcc atatctcttg gtctttattc
     9241 cttcttcagt cttttcagca gcttcaaata aggcacgaca gactgctact tcattatatc
     9301 ttaaattttt tatgtaaagc attgtcatta gataacccca tttattgtaa tggcaggca
//
LOCUS       DATNSN010000132         9334 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.132, whole genome shotgun sequence.
ACCESSION   DATNSN010000132 DATNSN010000000
VERSION     DATNSN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.132"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..824
                     /locus_tag="VAH76_002429"
     CDS             <1..824
                     /locus_tag="VAH76_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEO1387029.1"
                     /translation="IDYILALVIEKTKSAISLNFPKKNKITGLINEKYRLQSKEKKII
                     YSECSGYVQGIDYNQIKKIAYDHHVQIFILIRAGDYVYKKSPILELYSDKSADYELLA
                     PSIMLACININHARLLNQDIEFGFEQVAEIAVRALSPGINDPYTARQSVFLLGELFLY
                     IDQYDIEQDKLYYGKNIVGQYRGFTYKGIIDSALARLRQAATRDLTVILTIYDLVIQL
                     SSLLNNKSMIKALLSHTHSLKEIINNEKLSKYDEEAFNEREKQLTKIENYLSNSD"
     gene            1156..2418
                     /locus_tag="VAH76_002430"
     CDS             1156..2418
                     /locus_tag="VAH76_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387030.1"
                     /translation="MRNNKMGEKIPSFFHPDNKANSPQILLNTPLIIKDSELLDLLNA
                     PIDKDEKYHLLISCIRGHSPKYLNQRIMVKSHTTIEGDEHTPPHWTSLATKREYAYPT
                     DWNQNNELTTTLANIIATNELNFSSTQMAALFRELMIKGVDFTSIDQSPYLNLIHPKS
                     GNNPLKSLIAASLGDSEIIHLLHDFMSYIENKVPRDEQLLIINNQDNFEFEAMQPAEF
                     LLRLGHEDLALRLYRMGAQPTVQAFRLACSSYGTTPHYEEAMECIQFLMNSVHLDETD
                     LLEGKNSLQKASPAQYNEMLTRVLRSLQEDSKLDTGHSSLFEIIQKDYIENYQGKMFI
                     SFHDFCQKEAKTDHPLIQKYALLRIRQENVGNGLFSDYYRTESRHLAEKKWTLLNKNQ
                     RANQLTHDLIDLIDTKLGSLSTFPSAKL"
     gene            complement(2517..3020)
                     /gene="msrB"
                     /locus_tag="VAH76_002431"
     CDS             complement(2517..3020)
                     /gene="msrB"
                     /locus_tag="VAH76_002431"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO1387031.1"
                     /translation="MTKLLGVVFYLFIIPNVMAYIPQFNKSEQLKKLTPLQYQVTQNG
                     ATEKSFDNAYWNNKEEGIYVDIVSGEPLFSSNDKYDSGTGWPSFTKPIDTSYLVFHTD
                     RSYFFIVRTEVRSKYANSHLGHVFKDGPEPTGLRYCMNSAALKFIPKNEMEKAGYGEY
                     LYLFNKK"
     gene            3219..4211
                     /locus_tag="VAH76_002432"
     CDS             3219..4211
                     /locus_tag="VAH76_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEO1387032.1"
                     /translation="MNQKQIKNQLALKWALEHLALNDEFKIIDQQKIIETAYSTVHRI
                     TSSKSNVYYLKQTPEMLYLEPQTLAYLKEQRCGNIPQIIAENKHLNCFLMLSCGDQTL
                     RNLFKENIDLEMLKAGILNYTKIQQLVENQLRQLIKLGIPDWRLEKFSSLYDQLIQQE
                     ELLLSDGLSKNEITLLNKAHAICFNLCESLSKYQIPETINHCDFHENNMILNQKTGNV
                     SIIDWGETVITHPFFSLNGCLWNITFFYPIKQSDTIYEALQRQCITPWLGLHDETDLL
                     NALKIANQLNGVFAALSYKRIYDATMDQSQTVQQEHPGSIAGCLRSFLNHFHLA"
     gene            complement(4255..5466)
                     /locus_tag="VAH76_002433"
     CDS             complement(4255..5466)
                     /locus_tag="VAH76_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1387033.1"
                     /translation="MVMKAMRLFKGILFTFFGIIFCFNSHAALEHEKLVNYISLGEFS
                     RETAEIALKKMPPLDTLTVHYDLQLYKINYKTQAPDGNLTIASGLVAMPIHPVGQVGI
                     VSYQHGTRFERNDVPSRNNEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQA
                     ETLASSSIDMLLAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEYPDLPVS
                     AVAPGSAPYGWEETMHFVMLEPGPRATAYLAYFFYSLQTYKSYWSGFDEIFAPPYNTL
                     IPELMDGYHAVAEILQALPQNPLLIFQPKFSNGIISKTDRNTEILKINFNHYDFKPTA
                     PLLLVGTKGDRDVPYAGAEMAYHSFRKYSDFVWIKSVSDTLDHVQAHPFVLKEQVDFF
                     KQFERQEAMNK"
     gene            5771..6634
                     /locus_tag="VAH76_002434"
     CDS             5771..6634
                     /locus_tag="VAH76_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387034.1"
                     /translation="MVITMPREEEQAKKKLEEDKVQEEKLIRSTVNDYIREFSNMLHA
                     CEQRARRKPADRTALSAEKDAHTLREFLDDLQKAQQEGHGDLPILRTPAEWAAIDQMR
                     QRCVQYQIDPMQNHKMKNEKTEKPIHDSQVSEENLRRLQILDRYKETPRSEEEEEKER
                     KRLINLATSGNNTFIAYEDLEKIAKSGKPFFIPPNSKEDACLSCGNGRLYKGSLSEIR
                     MQLEFDLKKDPDNEQMRTGLRRIENEIFMKPNLASPKKEKDLTEAGITPQENTYTTPN
                     PFSIKPKKEPI"
     gene            complement(6921..7491)
                     /locus_tag="VAH76_002435"
                     /pseudo
     CDS             complement(6921..7491)
                     /locus_tag="VAH76_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7550..8718
                     /locus_tag="VAH76_002436"
     CDS             join(7550..7814,7814..8718)
                     /locus_tag="VAH76_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO1387035.1"
                     /translation="MVARKKYSKEFKLDAVSLVLEQGYGCTEAAKSLGIHQALLSRWL
                     KEHQEPDGQAFRGNGKLTAEQLEIRRLQEENKRLKMEKEILKKAGGLLCSRNEIKYSF
                     VAQNKKTWSVGLMCQLLGITRHGYYSYQQRQRNKPDDPKHEEVIDCVKKIAEASQYCY
                     GSRRMKNALNALGYPVGRRRAQSLMKEAKVFVRYRKKYKVTTNSNHKQSVYENVLNRQ
                     FHTDKPNKAYVSDITYIWTQEGGLYLAVVLDLFSRKVVGWSMGSRMKATLVCDALNMA
                     IWQRKPPSGLIVHSDRGAQYASCQYRNLLTGKGYIGSMSRKGDCWDNSVVESFFGSLK
                     QERVQWRNYQTRWEAQQDILNYITMFYNSHRLHSYLGYLSPNQFEKQSDFLMKVA"
     gene            complement(8845..9261)
                     /locus_tag="VAH76_002437"
     CDS             complement(8845..9261)
                     /locus_tag="VAH76_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEO1387036.1"
                     /translation="MFSIKSIDNYTGIIRPSGVIDKSLIKAEERGILRVLLSLLLQEN
                     TQSIIVKKINSSARYGRLKKALFEYNKILKSTHVLNLIDNMALRKAIRAARNRTEAYH
                     QLQGLKNISNPDVNTAIIIFTRLSISFPNLLNFGGL"
BASE COUNT         3236 a         1735 c         1648 g         2715 t
ORIGIN      
        1 agatcgatta tattcttgcc ttggttattg aaaaaacaaa gtctgctatt tcacttaatt
       61 ttcccaaaaa aaataaaata acagggctta taaatgaaaa atatcgccta caatcgaaag
      121 aaaagaaaat aatttactcc gaatgcagcg gttatgttca aggcattgat tataatcaaa
      181 taaaaaaaat tgcctacgat catcatgtgc aaatatttat tcttataaga gctggagatt
      241 atgtctataa aaaaagtccc atactagaat tatatagtga caaatcagca gattatgaac
      301 tcttggctcc atctattatg ctagcgtgca tcaatataaa tcatgccagg ctcttaaatc
      361 aggacattga atttggattc gagcaagtcg cagaaattgc tgtacgtgct ttatctccag
      421 gaattaatga cccttacaca gcaagacagt ctgtgttttt attgggcgaa ttattccttt
      481 atatcgatca atatgacatt gaacaagaca aattatatta cggtaagaac atagtgggtc
      541 aatacagagg cttcacttat aagggtataa tcgattccgc cttggcacga ttaagacaag
      601 cagcaacaag agatctgaca gtcatattga cgatttatga tttggttatt caactaagca
      661 gcttattaaa caataagtct atgatcaaag ccttattaag ccatactcat tcattgaaag
      721 aaatcattaa caatgaaaaa ttatcaaaat atgatgaaga agcttttaat gagagagaaa
      781 aacaactaac caaaatagaa aattatctat ccaactctga ttagaataat caagttttaa
      841 ttcgattgac gttggatatt agatcaagta ctcacaggca taggcctgaa aaaatgaaac
      901 acggtttgtc cttatattat ccaggcaact tagtaaaaca ctctatgaat ttttcatcaa
      961 gttttaactc tccatttttt catcagatag catgaaaata ttcttgactt atataaacaa
     1021 tctaatcaag atgaaatcat catgactaaa ataggactaa aaacagatct gttgaccgtt
     1081 acaaacaaaa catgggttcg aagcttttgt tgcattagct atcgctttta aactttttga
     1141 aacaggttct taactatgcg aaataataaa atgggagaaa aaattccaag tttttttcat
     1201 cctgataata aagccaactc acctcagata ttattaaaca ctccattaat cattaaagat
     1261 agcgaattgt tggacctgtt aaacgcccca attgacaaag atgagaaata tcacctctta
     1321 atatcctgca ttcgcggtca tagtcccaaa tatttaaatc aaagaattat ggttaaaagc
     1381 catacaacga ttgaaggtga tgagcatact cccccccact ggacatcttt ggcaacaaaa
     1441 agagaatacg cttatccaac tgattggaat caaaataacg aattgacaac aaccctggca
     1501 aatatcattg ctacgaatga attaaacttt tcttccactc aaatggctgc cttgtttaga
     1561 gagttaatga taaaaggagt cgattttacc tccatagatc aatcccctta cctcaattta
     1621 atccatccca aatctggcaa taaccctctc aaaagcttga tagcagccag tttgggtgat
     1681 tctgagatta tccacttact gcatgacttc atgagttata ttgaaaataa agttcctcgt
     1741 gatgaacaat tattgatcat caataatcaa gataactttg aattcgaagc aatgcaaccg
     1801 gctgaatttt tattacggct aggtcatgaa gatttggctt tacgcttata caggatggga
     1861 gctcaaccga ctgtacaagc attccgactg gcttgctcca gttatggcac cacccctcac
     1921 tacgaagaag cgatggaatg cattcaattc ttgatgaatt ctgttcatct ggatgaaacg
     1981 gatttattag aaggaaaaaa ttccttgcaa aaagcaagcc cggcacaata caacgagatg
     2041 ttgacaagag tattacgctc cctgcaggaa gattcgaaac ttgatactgg acactcctct
     2101 ctctttgaaa taatacagaa agactacata gaaaattatc agggtaaaat gtttatttct
     2161 tttcacgatt tttgtcagaa agaagcaaaa actgatcatc cactgattca aaaatatgcc
     2221 cttttgcgca tcaggcaaga aaacgtaggt aatggcctgt tctcagatta ttaccgcact
     2281 gaatccagac atttggccga gaagaaatgg actttactca ataaaaatca aagagccaac
     2341 caattaacac atgatctcat tgatttaatt gacactaaac ttggatcatt aagcacgttc
     2401 ccttcagcca agctataatt ggaggaacat acggcaagtt cagcccattt aagctctaac
     2461 attgatttga gctcaatgag ttccacaggc taacttgctg aaataacaag ggcagtttat
     2521 tttttattga acagataaag atactccccg tacccagctt tttccatttc attttttgga
     2581 ataaatttca aggcagctga attcatgcaa tatcgcaacc cggttggttc tgggccgtct
     2641 ttaaagacat ggcctaaatg agagttagcg tatttagagc gaacttcagt acgcacaata
     2701 aaaaaatagc ttctgtcagt atgaaaaact aaataggatg tatcgattgg ctttgtaaaa
     2761 ctgggccaac ccgttcctga atcgtattta tcattagaac taaacaaagg ttctcctgat
     2821 actatatcaa cataaatacc ttcctcttta ttgttccagt aagcattatc aaatgatttt
     2881 tccgttgccc cattttgggt tacctgatat tgcaatggag tcagcttctt aagctgctct
     2941 gatttgttaa attgaggaat ataggccatc acgtttggaa ttataaacag ataaaataca
     3001 acgccaagta gttttgtcat gtcataactc cacatgcact gttcatgccc acattgatga
     3061 tgcttactta tagtgtagca agcgtacccc atattagcga agagagtaat aaatctgcgt
     3121 ttccatcaag gatcaaaaca gaagataagc atgatgtttg gacttaaact attttataca
     3181 ttaaattcat attaaagatt attagttatg agcagactgt gaatcaaaag caaataaaaa
     3241 atcagctcgc cttaaaatgg gctcttgagc atctggcatt aaacgatgaa tttaaaatca
     3301 ttgatcaaca gaaaattatt gaaaccgctt attcaacagt acatagaatc acctcttcca
     3361 aaagcaatgt ttattatctg aagcaaacac cggaaatgct gtacctggaa ccccaaacat
     3421 tagcctatct taaggaacaa cgatgtggca atattcccca aatcattgca gaaaataaac
     3481 acttaaattg ctttttgatg ctgtcttgtg gcgatcaaac tttacggaat ctatttaaag
     3541 aaaatattga tttggaaatg cttaaagcag gtattcttaa ttacactaaa attcaacagc
     3601 tagtagagaa tcaactccga caactcataa aattgggtat ccccgattgg cggcttgaaa
     3661 aattttcatc attatatgac caactgatac aacaagaaga gcttttatta agtgatggac
     3721 tatcaaaaaa tgagattacc ctcctgaaca aagcacacgc aatttgcttt aatttatgtg
     3781 aatcactctc caaataccaa attccagaaa caattaatca ttgtgatttt catgaaaata
     3841 acatgattct caatcaaaag acgggaaatg taagcatcat tgactgggga gaaaccgtta
     3901 tcactcatcc atttttttct ctgaatggct gcctgtggaa cataaccttc ttttacccaa
     3961 taaaacagtc cgacacaata tatgaagcat tacaacgaca atgtattact ccttggttag
     4021 gtctgcatga cgaaactgat ttattaaatg cgctaaaaat tgccaaccaa ctcaatgggg
     4081 tttttgctgc attaagttat aagcgaattt atgacgcaac tatggatcaa tctcaaacag
     4141 ttcaacaaga acaccctggc tccatagctg gctgcctaag atcattttta aaccattttc
     4201 atttagcttg actgctacat caaaactgtg attgctgagt ttcataaaat gatattactt
     4261 attcatcgct tcttgccgct caaattgttt aaaaaaatcc acctgttctt tgagtacaaa
     4321 gggatgagct tgcacatgat ccaaggtgtc actgacagat tttatccaca caaaatcact
     4381 atatttccta aaggaatgat atgccatttc agctcctgcg taaggtacat cacgatcacc
     4441 tttagtgcca accaataaca aaggagctgt aggtttaaaa tcatagtggt taaaattaat
     4501 ttttaaaatc tcggtgtttc tatctgtctt actaataatt ccattagaga acttaggttg
     4561 aaaaataagc agaggatttt gtggtaacgc ttgaagaatt tcggcaacag catgatatcc
     4621 atccatcaat tccgggataa gagtattgta aggaggagca aaaatttcgt caaaaccaga
     4681 ccaataactc ttataagttt gcagagaata aaagaaatag gccaaatagg ctgtagcgcg
     4741 aggtcctggt tccaacatca caaaatgcat ggtttcttcc cagccataag gagctgaacc
     4801 tggggcaact gcagagacag gtaaatcagg atactctttt gcaagcattt caaacattac
     4861 aatagttgag aatccaccct ctgaatagcc tgccagatat aacttatcac ttattgggta
     4921 atgcagcctg ttggctaact ccttagcagc aagcaacata tcaatgctac tgctagcaag
     4981 agtttctgcc tgaacataag gatgaagtgt taattcatta tcacccagac caagataatc
     5041 tggcatcacc gtcatataac ctgcactatt accataggca gcaaggtata tatagttttt
     5101 ttcattattt ctggaaggta catcattgcg ttcaaatcgg gtgccatgct gatagctaac
     5161 aattcccact tgccccacag gatgaattgg cattgcaact aatcctgagg caatagtcag
     5221 atttccatct ggtgcctggg ttttataatt aattttatat aattgcagat cataatgtac
     5281 tgtaagagtg tctaatggcg gcattttttt taaagcgatt tctgctgttt ccctggaaaa
     5341 ttcacccaaa gaaatataat taaccagctt ctcatgctct agagcagcat gggaattaaa
     5401 gcaaaaaata atcccgaaaa atgtaaaaag aatccctttg aataaacgca tcgctttcat
     5461 aaccatctat aaaaatgtgg ataattacat gtttaataag gagaatcaaa tgaagcagtt
     5521 acttgcatta atgcaaagga cacaatctgt tcctttctat ttattccaat catcaaacaa
     5581 actagtattc aattaaaacg cctgatgaaa taaatgaagt attatctaca aatgctcttg
     5641 ttgctgaaaa aatgcatgaa ttttatagga aaactcagag agataagtaa ctccagaaaa
     5701 atatactatc attaatttta taagataaga ttattttcaa ttctacagtg aattgatgtt
     5761 caaataattt atggtaatca caatgcccag agaagaagag caagctaaga aaaagttaga
     5821 agaagataaa gtacaggaag aaaaactcat ccgaagtact gtgaatgatt atatccggga
     5881 attcagtaat atgttacatg cttgcgaaca acgagcaaga agaaaacctg ccgacaggac
     5941 agcactctca gccgaaaaag acgcgcatac tcttcgtgaa ttcctcgatg accttcagaa
     6001 agctcaacaa gaaggacatg gagacctacc tatactacga accccggccg aatgggctgc
     6061 tatcgatcaa atgcgacaaa ggtgcgtaca gtaccaaatc gatcccatgc agaaccataa
     6121 aatgaagaat gaaaaaacag agaaaccaat ccatgactct caggtttctg aagaaaattt
     6181 acgccgacta caaatattag acagatacaa agaaacacct cgctctgaag aggaagaaga
     6241 gaaagaaaga aaaagactta taaatcttgc aaccagcggt aataatactt ttattgctta
     6301 tgaagatttg gaaaaaatag ccaagagtgg taaacctttt tttatcccac ccaactcaaa
     6361 agaggatgcg tgtctatctt gtggtaatgg gcgtttgtat aaaggttcat tatcagagat
     6421 ccgtatgcaa ttagagtttg acctaaaaaa ggatccggat aatgagcaga tgagaacagg
     6481 tttgagaaga attgaaaatg aaatttttat gaaacctaat ttagcatcgc ccaaaaagga
     6541 aaaagacttg actgaagcag gaataactcc acaagaaaac acttacacta ctccaaaccc
     6601 attttcaata aaaccgaaaa aagaacctat ataagtctat gtactgatga gcttaaccac
     6661 ttggagccaa acaactggaa atcttgctaa agtgatgaaa ctcaatggtt agtaacataa
     6721 gctttctatc aaccaaccaa gaaaatgccg caccgctatc aagacaaaca aaacacagtt
     6781 atttaaacac gactggtaga acagagacat ggctatgaga acaacttgtg ctgctacagt
     6841 ctgcgccaaa ttgaaaaatt taaattgaat ttgatcaggc aagtcagcgc ataaatggca
     6901 actgcccgat caaatttgag ctacttcatt gcaagcttgg gtgattgtaa atctttgagt
     6961 gccttgctaa aaatctcttc cactgcccgt tgcgaatcag tttttatccc ggttactttc
     7021 agtaaataac ccttgggctt tgctaagggt catatattcg gagctttgtt taaatttctt
     7081 ttcaaactct tttaaatcat ctaaattacc tattttttct atatcatttt ttaaattatt
     7141 gattattgcg cgttttaata tatctccttt tacttgactg tatctttctt taaaagattt
     7201 gtcgcctatt ttttcatcta cctttgctgt aaaaagatga aagcgtgaac ctatttttcc
     7261 gttcaggtat tcttttacag caatatcgca attagttaat atttccttaa aatattcacc
     7321 ccatttaaat tggcctggct cttcattatg gtattttaca agttgggcgc gtccagcatg
     7381 tttaagccca ttacacatct ccaggccatc tcccttacta tcatcacaaa tcaaacgaaa
     7441 tcctggtaaa cattcaggtt tgttttcgtg tgataccact gcttcgtgac gtggtctaat
     7501 agattttgac accccagtta agagataatg tatttaactg gagaaaaaaa tggtagcaag
     7561 aaagaagtat tctaaagaat tcaaacttga tgcagttagt ctggtattag aacaaggcta
     7621 tggctgcaca gaagcggcta aaagtttggg gattcatcaa gctctactta gtcgctggct
     7681 taaagagcac caagagcctg atgggcaagc atttcgagga aatggcaagt taacagcaga
     7741 acaattagag atacgtcgcc tgcaggaaga aaataaacgc ttgaaaatgg aaaaggaaat
     7801 cttaaaaaag gcggcggtct tctttgctca agaaacgaaa taaaatattc gtttgtcgcc
     7861 caaaataaga agacctggtc ggttggctta atgtgccaac tcctgggcat tacacggcat
     7921 ggatattaca gttatcagca acgccagaga aataaaccag atgatcctaa acatgaggag
     7981 gttattgatt gtgtgaagaa aatagctgag gcgagccagt attgttatgg aagtcgcagg
     8041 atgaagaatg ctttaaatgc attaggttat ccagtaggtc gaagacgagc tcaaagttta
     8101 atgaaagaag ccaaagtttt tgttcgatat cgaaagaaat ataaggtgac taccaatagc
     8161 aaccataaac aatcggttta tgaaaatgta ttgaataggc aatttcatac agataaacct
     8221 aataaagcct acgtatcaga tattacctat atctggacgc aggaaggcgg gctttattta
     8281 gcagtcgttc tggatttatt ttcaaggaag gtggttggtt ggagcatggg atccagaatg
     8341 aaagcaacat tggtttgtga tgctctgaat atggcaattt ggcagcgaaa acctccatca
     8401 gggcttattg ttcattccga tagaggggct cagtatgcta gttgccaata ccgcaattta
     8461 ttaacgggca aaggatatat tggtagcatg agcagaaaag gtgattgctg ggataacagc
     8521 gttgttgaaa gcttctttgg cagcttaaaa caagagcgag ttcagtggcg aaattatcaa
     8581 actcgatggg aggctcaaca ggacattttg aattacatca ccatgttcta taacagtcac
     8641 agattacatt catatttggg ttatttaagt ccaaatcaat ttgaaaaaca gagcgatttt
     8701 ttaatgaaag ttgcttaact gaggtgtaac attttggttg accacgtcaa agttaaatgg
     8761 ttcatttaag cggtggtttt tttaaactca aaaacatcca tattgaagta atacaaaaga
     8821 aataggatca tacatatagg gaatttacag acccccaaaa tttaataaat taggaaagct
     8881 gatggataat ctagtaaaaa ttattattgc agtattaacg tcaggattgc ttatattttt
     8941 gagaccttgc aattggtgat aagcctcagt ccgatttctt gctgcacgta tggctttcct
     9001 aagcgccatg ttatctatta aatttaatac atgcgtactt tttaatattt tattgtactc
     9061 aaaaagtgct tttttcaatc ttccgtaccg agcagaagaa tttatttttt tgacgatgat
     9121 actctgagta ttttcctgca aaagtaagga gagtaaaact cgtaatatcc ctctttcctc
     9181 agctttaatc agacttttat caatgacccc actaggacga ataattccag tgtagttatc
     9241 aattgattta attgaaaaca agtcattagc cgcttctggc ccctggggag taacggaaca
     9301 gccaactcac ttaagagaat tatattatta aaaa
//
LOCUS       DATNSN010000133         8964 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.133, whole genome shotgun sequence.
ACCESSION   DATNSN010000133 DATNSN010000000
VERSION     DATNSN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.133"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            186..1382
                     /locus_tag="VAH76_002438"
     CDS             186..1382
                     /locus_tag="VAH76_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEO1387037.1"
                     /translation="MLEYLTWITANIVLVSNSIFGTPHPVHAQLQVPQCLAAKMTTAH
                     DVLAENEQFKIIDLPGSDIENVTLLAEQVNCGDFIDVTNKFSDTLLAAKRKSAENILQ
                     SSALSALKKSQENKNFYEIKHQKEVNAAIKEVVADNIWQTLNHLTSYSNRSATKDTGV
                     AAANWLKSQVDAMATEYDRSDTATFFVKAGWYKQPSVVTVIGKELKTPAIVIGAPMDT
                     LDGHMPGADEGSGAAAIMESTRVLLASKLKFKHPIYIIWYAASDRNLAGSQYVVQYFQ
                     EKSIPVKAVLQLNKTGFRANSDDSTMWVFTDNTNNDLNQYIAKLIKNYIRTPVNFSQC
                     NYECGDHISWSQEDIPVTYAVESDFKNLNPYIGSSSDTMDFLNLEHIANFSKLAIAFS
                     IELGSK"
     gene            complement(1440..3119)
                     /locus_tag="VAH76_002439"
     CDS             complement(1440..3119)
                     /locus_tag="VAH76_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate decarboxylase"
                     /protein_id="HEO1387038.1"
                     /translation="MCSIGEYLAKRLEELNISEYFAIPGDYNLILLDEVLKNEKLKMI
                     NCCNELNAGYAADGYARVKGVSALFVTYSVGGLSTVNAVAGAYAENLPILVISGGPNT
                     NSIQDAEILHHTLATEDNLYVRDIFSRITAHVVCIRKPEQAAMQIDEAIAIAIAKKKP
                     VYIEIACNIANYPISKPTQRAFNTYKISDSSSLAAAVEHAAEKLNSALKPSLIVGSKV
                     RHCNAINSVSELAKCTGYAMAAMPDAKGFISEQHPNFIGIYWGPVSSSGCAEIIDSSD
                     AYLLIGPNENDYTTVGYVWGINPHKSIKTTKGGVIIGETVYTNIFMNDFLRELSKKLK
                     FNDASYKAYKRIAEETVVYPELDQPDAPLSSRYLFGQIQKMLSDKKALLAETGDSWFN
                     CMRLSLPEGCPFEIQMQYGSIGWSVGALLGMQAALHNQKRVIACIGDGSFQMTAQEIS
                     TMIRYGYKPIIFLMNNACYTIEVQIHDGPYNVINNWRYAELVNVFRGEQSNAKSFVVK
                     TNKELLTAIKQAEKTDALCFIEVFLDKDDCNKNLLEWGSRVANYNSRPPRR"
     gene            complement(3359..4441)
                     /gene="ravQ"
                     /locus_tag="VAH76_002440"
     CDS             complement(3359..4441)
                     /gene="ravQ"
                     /locus_tag="VAH76_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavQ"
                     /protein_id="HEO1387039.1"
                     /translation="MMRMFSVRRNPSAVMRKDLSLTSRPLLQAPLLKSSVNPQNTLKN
                     KELKERKFTETLPDSRFFSKLKLAEQDQFSHRKRKRFNYQKNFSAALSLPYSRKELDV
                     HRDEEGALLSMVKKYVIEHANRRVDLFFYTLIAYYNTEIVPTNGYTDLQHGRGRNTNR
                     GRTITEACHSSLTPSLLDSTIYQNKGRLKHKSLLSGTHFMDSLNATVELPPFVNDFDD
                     ILEKTCRKKCLKILHEVSLGRINPMEGLTLFLKMMQDILIKFKEEAESENQSVLLQNS
                     LIRKKFISPNLIDLVINGTLLTTFSDSTQSASDDYIQLLLRMTSEEKALCEKDEKGKI
                     KLYLAKIMDIQNEILTSKNGEFVPGL"
     gene            4887..6374
                     /locus_tag="VAH76_002441"
     CDS             4887..6374
                     /locus_tag="VAH76_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1387040.1"
                     /translation="MQTKLLQFICIKKLCKQKTPYKSKIKFVSLTLAKKQIICIITLL
                     SIIKWSWATHHYDTIIIGAGVSGLTAAHHLHNAKQKVLVIEAKNRLGGRVYTSYDWGF
                     ATDLGASWIHAIENNPLLPLIGKQSIIINSYSNSDPVAMLTNYALYDSEGKPVSKLTQ
                     DLFSSLTREFLRYCQTRSQMISFAQNLTTFAKQKKLTADQLALLSYALENIYTYEFAD
                     NLTKLSRNVHSVSEASTTSGKNALVPEGYFQLFRRFTQHIPIHLNQIVSQINYGPDGV
                     NIITQHEKYHANQVIITVPLGVLKANAIKFHPALPKDKRTAISQLGMGSYEKLYLLFD
                     KVFWDKDKEWIGMLPQNEQEAFNIFNYYKYTKKPVLIVFTSGKLARDMEKEHLTEWVM
                     QHLRRIYGNNIPKPIKNKKTHWGSDPFTRGSYSYLPVNVDKSVIGTLAQPVANRLYFA
                     GEATSTTDPSTVHGAYLSGIRAAEEVLASIKHSEKNRERNKAVNS"
     gene            6274..7224
                     /gene="ravP"
                     /locus_tag="VAH76_002442"
     CDS             6274..7224
                     /gene="ravP"
                     /locus_tag="VAH76_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavP"
                     /protein_id="HEO1387041.1"
                     /translation="MALIYQGYAQQKKYWPASNTLKKIEKEIKLSIHKPILILEKVMY
                     TKRDNHLLINKEEEQEENQKTVFVPQTNSLTLPETQSYFCTAEQCSLNNPYAPAQGYT
                     RPDSFHFKTAPINSEIYEQVFKVSEETVKTETQFANRVPGHLEKEENRSGDWSGLIPD
                     ELYQMMLEAAKQGIFVSFLLTLTDEIINDYLKNHHYSQQQINWISQGIRALTLIAIGT
                     SPGIALGLSISTLLLTEHLGFDKTNVNYLTTSSVVAWGLLSSPFNLLNTSLVMASSIG
                     ASVLTSKITQSSYQFVRNRFFSSKPEKEELEHEHILQKTI"
     gene            7536..8846
                     /locus_tag="VAH76_002443"
     CDS             7536..8846
                     /locus_tag="VAH76_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387042.1"
                     /translation="MKINIRGVDKADVLAALYNGAHGGLAGGFSYLQGEITPQQAKLL
                     VEKQTNLYFDYVDNRPLKIDLSGDVIDTTLYDHYNGGCAAQRALSHLTLPSDQPVEEI
                     KKQDPGKVCEHIFRTYIQPVDGYPADAYGDNLRALQYAHKACGVDRYFFRDFLNREYT
                     EQGGGLFGLGLISRYTQRRIILKSDPSAGLEILYACFSFIRDKEDSLLNELLSAENPD
                     GPNSQKIARALVGDLEYLRDSRLRWLSTGRQPALIDDFLKDRRKASIVLTAILRGESA
                     PEALKLIMDHITSGNTALDNIVVYNSLKVLQEKISVIPNECIRLELFNILDESIQKHS
                     YSNVLFAMEQSIKKVSSSWNYNNIKEKSEALARLRLNFLNDPSQENYQAFISEAAKPR
                     KSWIFQAQEGKTSSLIAFNNALSEKTKQIYEGNGDYTFSAGMST"
BASE COUNT         2864 a         1784 c         1607 g         2709 t
ORIGIN      
        1 taattttgtt aattttgtag tatatttgtc catgctattt tggagataaa acacatgacc
       61 agtaaattgg tcatttacaa tatataggaa aaaataacga actctccaaa attatttttt
      121 aatacttgga ataatgtgct ttgcccatga atcgatacgg attctacagt tgtttaggag
      181 tgtaaatgct tgaatatttg acatggatta ccgcaaatat tgtattagtg agtaattcaa
      241 ttttcggaac accgcatcca gttcatgctc aattacaagt acctcaatgc cttgctgcaa
      301 aaatgacgac tgcgcatgat gtcctggctg aaaatgagca attcaaaatc attgatctcc
      361 ctggcagcga tattgagaat gtaactcttt tagcagaaca agtgaattgt ggcgatttta
      421 tcgacgtaac gaataagttc tcggatactc tgttggctgc caaaagaaag tcagcggaaa
      481 atattctgca aagcagtgca ttaagtgccc ttaaaaaatc tcaggaaaac aagaactttt
      541 atgagattaa acaccaaaag gaagtcaatg ctgcaataaa agaggtagtt gctgacaaca
      601 tttggcaaac attaaatcac ttgacctcct atagcaatcg ttcagcaacc aaggacacag
      661 gagtagctgc cgccaattgg ttaaaatccc aggtggatgc aatggcaacg gagtacgatc
      721 gcagcgatac tgccacattt tttgtgaaag ctggttggta taagcaacct tccgttgtca
      781 cagtcattgg taaagaactg aaaacacctg ctatagtcat tggagctcca atggatactt
      841 tagatgggca catgcctggc gctgatgaag gtagcggtgc tgctgccatt atggaaagca
      901 ctcgtgttct gttagcctcc aagctgaaat tcaagcatcc gatttatatt atctggtacg
      961 ctgccagcga tagaaatttg gctgggtctc aatatgtagt ccaatatttt caagaaaaat
     1021 ctattcctgt taaagccgta ctgcaactca acaagacagg gtttcgcgct aactcagatg
     1081 attcaaccat gtgggtattt accgacaata caaacaatga tctgaaccaa tatattgcaa
     1141 aattaataaa aaattacatc cgtacaccag ttaatttttc gcagtgtaat tacgaatgcg
     1201 gtgatcatat ttcctggagt caagaagata tacccgtaac ctatgcagtt gaatctgact
     1261 ttaaaaacct taacccctat atcggcagct cttcagatac aatggatttt ttgaatctgg
     1321 aacatatagc taacttttca aagctggcca ttgccttctc aatagagctg ggctccaaat
     1381 aaaaacacgt caaatcatta atatcagggt aggcgattct tataattcct acccaagatc
     1441 tatctacgag gaggacggct attataattt gccactcttg agccccattc caacaagttt
     1501 ttattgcaat catccttgtc caggaacact tcaataaaac aaagcgcgtc tgttttttct
     1561 gcctgtttaa ttgcagtgag tagttccttg ttggttttaa ctacaaaaga ttttgcatta
     1621 ctctgttccc cgcggaatac attaaccagt tcagcgtaac gccaattatt aatgacatta
     1681 taaggaccat catgaatttg tacttcaata gtgtaacaag cattattcat taaaaaaata
     1741 attggtttat acccgtaacg tatcatggtt gatatttcct gggcagtcat ttgaaatgag
     1801 ccatctccta tgcaggcaat cactcttttt tgattatgca aagcagcttg catgcctagc
     1861 aaagcaccta ctgaccaacc aatagaacca tactgcattt gaatttcgaa aggacagcct
     1921 tcaggcaaag acaggcgcat acaattaaac caggagtccc ctgtttcagc caacaaagct
     1981 tttttgtcag aaagcatttt ctgaatttgg ccaaacaaat aacgactgct taatggagcg
     2041 tctggttgat ctaactctgg ataaacgaca gtttcttcag caattcgttt ataagctttg
     2101 tatgaagcat cattaaattt taatttttta cttaattctc tgagaaaatc attcatgaag
     2161 atattagtat aaactgtttc accaatgata acccctccct tggtggtctt tattgatttg
     2221 tgggggttga ttccccatac atacccaact gtagtatagt cattctcatt aggcccaata
     2281 agcagatagg catcactgga atcgataatt tcggcgcatc cagaagagct gactggtccc
     2341 caatagatac ctataaagtt gggatgctgt tctgagataa atcctttcgc atcgggcata
     2401 gccgccatag catatccagt acactttgcc aactcgctga cagaattgat tgcattacaa
     2461 tgcctaacct tactacctac aatcaagctg ggctttagag cggagtttaa tttttcagca
     2521 gcatgctcta ctgctgcagc cagagacgaa ctgtcgctaa ttttataggt gttgaatgcg
     2581 cgttgagtag gcttggagat aggatagtta gcaatattac acgctatttc aatatagacc
     2641 ggcttttttt tagcgattgc gattgctata gcctcgtcta tctgcatggc agcctgctct
     2701 ggctttcgaa tacaaacgac atgggcagta attcgagaaa aaatgtccct tacatacaaa
     2761 ttatcttcag tcgccagcgt atgatggaga atttcagcat cctgtatgga attagtattt
     2821 gggccacccg atataaccaa aatggggaga ttttcggcat acgctcctgc aacggcatta
     2881 acagtactaa gtccacccac actataagta acgaataaag cggagacccc ttttacccgg
     2941 gcatagccat ccgcagcgta tcctgcattc aactcattac agcaatttat catttttaat
     3001 ttttcgtttt tcaggacttc atcgagtaaa ataagattgt aatcacccgg tatagcaaaa
     3061 tattcagaaa tatttaattc ctccagccgc tttgccaaat actctccaat tgagcacatg
     3121 ttaatccctt attaaataag aactttttaa attatagcac agccaaaaat cagtaggtgg
     3181 caagtgaata cctgctagaa aatagagttc acaaagcatc aaattgcaaa tgataattta
     3241 aatattctat cttggagtat gttgacaatt cgatccagta actacatccc gcgtcttgag
     3301 cgcgggatta atggatgctg tggataaaca aacaggtcgg catttttgtt aacacatctt
     3361 aaaggccagg cacaaattca ccatttttcg atgtcaaaat ctcattttga atgtccatta
     3421 tttttgccaa atacaatttg attttgccct tttcatcctt ttcacaaagc gccttttctt
     3481 ctgatgtcat tctaagtaaa agttggatgt aatcatcaga ggcgctttgt gtgctatccg
     3541 aaaatgtggt taataacgtg ccattaataa ccaaatcaat caggtttggg ctgatgaatt
     3601 ttttccttat caatgaattc tgcaatagta cagattgatt ttcgctttct gcttcttctt
     3661 taaattttat taaaatatcc tgcatcattt ttaggaaaag ggttaatcct tccattggat
     3721 tgattcttcc aagtgatact tcatgcagaa ttttcaagca ttttttcctg caagtctttt
     3781 ccaggatatc atcgaaatca ttcacaaagg gaggtaattc taccgtagca tttagactgt
     3841 ccatgaagtg agttccggaa agaagacttt tatgttttaa tcttcccttg ttttgataga
     3901 tagtactatc aagcaatgat ggagtaagac tgctatggca ggcttcagtg atagttctgc
     3961 ccctgttggt attacgcccc ctgccatgct gaagatcagt atacccattt gttggaacga
     4021 tttctgtatt ataataagct atcaatgtat aaaaaaataa gtcaacacga cgattagcat
     4081 gttcaatgac atactttttc accattgaca gcaatgcgcc ttcttcatcc cgatgtacat
     4141 ctaattcttt tcttgagtat ggcagtgaga gtgctgcact aaaattcttt tgataattaa
     4201 atcgcttacg ttttctgtga ctaaattgat cctgttctgc caattttaat tttgaaaaaa
     4261 accttgaatc cggtaatgtt tcagtaaatt tacgctcttt taactcttta tttttcagag
     4321 tgttttgagg gttaacacta ctcttaagca gcggcgcttg gagtaaaggg cgggaagtta
     4381 aagaaagatc tttgcgcatt actgcggaag ggtttctacg aacactaaac attctcatca
     4441 tggtcttctc ccttaatagc cattatgtca ccgtagtacc tgctcaaagc atcaagcgtc
     4501 tgtttttcca taaagacact taaacttact tagtgagagt tcacagtctg gatgatcttc
     4561 aaagccagct ccctgtctaa gccagaacat atactgtctt tttacagtac ataataatct
     4621 ttaaaaaagc aaacattaaa ataacttaat attgaatgct tcatacagac aagttaattg
     4681 ctatgctgat tattatttac aaatattgat acttttttta atatgcatca attcaataac
     4741 aaggatttac tgtgtcatac cagacttttt ttatcgactt gatcatcata gttgaatctt
     4801 ctgagatcgc atgcagcaaa acttacgcaa ggttttagtt aatccagtca agcacgtaaa
     4861 aaaatagcga tccaggagtg aaagacatgc aaaccaaatt attacagttt atttgcatta
     4921 aaaaattgtg caaacaaaaa actccttata aatcaaaaat aaaatttgtc agtttgactt
     4981 tagcaaagaa acaaattatc tgcatcatca cattactttc cattataaaa tggtcctggg
     5041 caactcatca ttatgacaca ataattatcg gtgctggggt atccggatta accgcagcac
     5101 atcatttaca caatgcgaag caaaaagtgc ttgttattga agcaaaaaac cgccttggag
     5161 gcagggtata taccagctat gattggggtt tcgccactga tttgggagca tcctggattc
     5221 atgccattga aaacaatcca ttactgccat tgattggtaa acagtcgatt attatcaata
     5281 gttacagtaa ctctgatcca gtagctatgc tgaccaatta tgctttatac gacagcgaag
     5341 gaaagcctgt atcaaaactg acacaggatt tgttttcaag tttaaccaga gagttcttaa
     5401 gatattgcca aactcgcagt caaatgatct ctttcgcgca aaatctcact acctttgcaa
     5461 aacaaaaaaa acttactgct gatcaacttg ccttactgag ttatgccttg gaaaatatct
     5521 atacttacga attcgctgat aatttgacta aattatcacg caatgtccat tccgtatccg
     5581 aggcatccac tacttctgga aaaaatgccc ttgtacctga aggctatttt caacttttcc
     5641 gccgatttac tcagcatatt cctattcatt taaaccaaat agttagccaa attaattacg
     5701 gacctgatgg tgtcaacatc ataactcaac atgaaaaata tcatgccaac caagtcatta
     5761 ttaccgtccc attaggcgtg ctcaaagcca atgcaatcaa atttcatcct gctttaccca
     5821 aagataagcg cacagcaatc tctcagttag ggatgggaag ttatgaaaaa ttgtacttat
     5881 tatttgacaa ggttttttgg gataaagaca aggaatggat tggcatgctc ccccaaaatg
     5941 agcaagaagc attcaatatc tttaattatt acaagtacac aaaaaagcct gtactcatcg
     6001 tatttaccag tggcaaactt gcgcgcgata tggaaaaaga acatttaacc gaatgggtga
     6061 tgcaacattt aagacgaatt tatggaaaca acatcccgaa accaattaaa aataaaaaaa
     6121 ctcattgggg cagtgatcca ttcacacgtg gcagttattc ttacttgccg gtaaacgttg
     6181 ataagagtgt tattggcact ctcgctcaac ctgtagctaa ccgcctctat tttgctggag
     6241 aagctacctc aaccacagat cccagtactg tgcatggcgc ttatctatca gggatacgcg
     6301 cagcagaaga agtactggcc agcatcaaac actctgaaaa aaatagagaa agaaataaag
     6361 ctgtcaattc ataaacccat cttgattttg gagaaagtaa tgtacaccaa aagagataat
     6421 catttgctga ttaataaaga ggaagaacaa gaagaaaacc aaaaaacggt atttgtccct
     6481 caaacaaata gtttgactct tcctgaaacc caaagctact tttgtacagc agagcaatgc
     6541 tctttaaata acccctatgc cccagctcaa ggttatacaa ggcctgattc atttcatttc
     6601 aaaacagccc ctataaactc agagatttac gaacaagtat ttaaagtttc agaagaaact
     6661 gttaaaaccg aaacacaatt tgcaaataga gttcctggac acttggaaaa agaggaaaat
     6721 cgttccggtg attggagtgg gcttattccg gatgagttat atcaaatgat gctggaggct
     6781 gcaaaacaag gcatttttgt ctcgttcctg ctcactctca ctgatgaaat aataaatgac
     6841 tatctaaaaa atcatcatta ttcacagcaa cagataaatt ggatctctca aggcattcga
     6901 gctttaactt taattgctat tggtacttca ccaggaatcg ctttgggatt atcgataagc
     6961 acacttttgc tgactgaaca tttgggcttt gataaaacga atgttaatta tctcacaaca
     7021 agttcagtcg tagcttgggg gctattaagc tcgcctttta acttgcttaa tacctccctt
     7081 gtgatggcaa gctctattgg agccagtgta ttaaccagta aaataacaca atcatcctat
     7141 caatttgtgc gtaaccgttt tttttcttcc aaacctgaga aagaagaact cgaacatgag
     7201 cacatattgc aaaaaacaat ctagcggtaa ttcactgcaa aattttttat gaattgctta
     7261 atacttttac taataaacta aatccggaag gcaggcaaat gattttcctc caacgagcca
     7321 aaaatatttt aggctttcct gaagaggaaa atctcttcca ccaactccat ccaaaaccat
     7381 ctatcgtgag ctatttcaat gcccttcctt gctcattgag caccaaaaat ttaagtttaa
     7441 tccgttgaca aaaactgttg cacttgattt tctcaccact aaaagtgtat aatttattca
     7501 ttttgtgcat ttattatatt tttgtaggtt actccatgaa aataaatatc cgaggtgtgg
     7561 ataaagctga cgtactggcc gcgttatata atggcgctca tggtggcctt gcaggtggtt
     7621 ttagctattt acaaggagag ataacaccgc aacaagcaaa attattagta gagaaacaaa
     7681 caaatcttta ttttgattac gtagataacc gccctttaaa aattgatctt tctggagatg
     7741 tcatagatac cacgctctat gatcattaca atggaggttg cgcagctcaa cgtgcgttgt
     7801 ctcatcttac attgccctct gatcaaccgg ttgaagaaat aaaaaaacag gatcccggga
     7861 aagtgtgtga gcatatcttt agaacttata ttcaaccagt ggatggatat cctgctgatg
     7921 cttatggaga taacctgcgc gctttacaat atgcgcataa agcatgtggg gttgatcgat
     7981 atttttttag agacttcctg aaccgtgaat acactgagca aggcggtggc ttatttggat
     8041 taggtttgat tagccgttat acccagcgta gaattatatt gaaatccgat cccagcgctg
     8101 gattagaaat attgtatgcc tgtttttctt ttataagaga caaagaagac agtttattga
     8161 atgagctttt atctgctgaa aaccctgatg gacccaattc tcaaaaaata gcacgtgctt
     8221 tagtcggaga cttggagtat ctgcgtgata gtcgattgcg ctggttatca acgggtagac
     8281 agcctgcgct tatcgatgat tttcttaaag acagaagaaa agcttctatc gtactcactg
     8341 ctatacttcg gggagaatca gccccggaag cattaaagct tattatggat catattactt
     8401 caggcaatac tgcactggat aatattgttg tatataacag cctgaaagta ttgcaagaaa
     8461 agatatccgt tatcccaaat gagtgtattc gattagaact attcaacata cttgatgaat
     8521 cgattcaaaa acactcttac agtaatgttc tttttgctat ggaacaatcc atcaaaaaag
     8581 tcagctcaag ctggaattac aataatataa aagaaaagtc tgaagcctta gcacgcttac
     8641 gattaaattt tttaaatgat ccttcacagg aaaattatca ggcatttatc tctgaagcag
     8701 ccaaaccacg taaatcgtgg atattccagg ctcaagaagg taaaacttca tcgcttattg
     8761 cattcaataa cgctttatct gaaaaaacta aacaaattta tgaaggaaat ggggattata
     8821 cttttagtgc cggaatgagt acttagaaat aatcacttcc caccttttag tagacagctg
     8881 tacatctgaa cattctaatc ctgatcaaag atagttgaga ccattatcat gagaaaaatg
     8941 gataatcaag ctactgccct atac
//
LOCUS       DATNSN010000134         8864 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.134, whole genome shotgun sequence.
ACCESSION   DATNSN010000134 DATNSN010000000
VERSION     DATNSN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.134"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            14..1651
                     /locus_tag="VAH76_002444"
     CDS             14..1651
                     /locus_tag="VAH76_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEO1387043.1"
                     /translation="MKKISCSILLLINSLISYADSDQPKLILQIVVDQLRGDLIYRHH
                     KQFGPSGFNYLLAHGLDYHNAHHPHANTTTCAGHSTIATGSYPSLHGIVDNDWYDRKT
                     RKLKYCMEDLQAKILPTVHTHIEISGRSPRNLKPSTLSDEIILANKGKAFGVSLKDRA
                     AITLAGHAGKAFWFDKTNGGFITSTYYYSSYPLWVENWNKHYQPKAYDWNLSHPKSFY
                     QNANTLPFRHNYGSFGQTFPHHVTNPPSEEYFKSLSRTPKADELTADFAKQLLVNEKL
                     GKTTGQTDYLAISFSAVDAIGHQFGPNSLEAEDNLIALDDTLSQLFKTIDKEVGLNNT
                     LIILTADHGVSDGPSYLKAHKIPEIKPVNIEATGKHIENLLLKRFNLPAQALMSINPP
                     FIYLDHQIIKEKNLDIAEVSFYLAEEISHLPGIFKAYPLPIRDIEKDWLSAKVDRMSY
                     AYRAGDVYIVQPPYQSHGAKSEDRVAHGSPWQYDTYVPLLFVHPDFKPKRIFRQVHTT
                     DIAPTLSSLLLIKPPAAAVGQPLVEVVNAFHKTGEND"
     gene            1704..2342
                     /gene="udk"
                     /locus_tag="VAH76_002445"
     CDS             1704..2342
                     /gene="udk"
                     /locus_tag="VAH76_002445"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO1387044.1"
                     /translation="MTKQAIIIGISGPSASGKSLLANTIVNELGSEQVVVISEDAYYK
                     DNGHLPFTEREKINYDHPDSFDHALLYEHLRQLRVGNTVQIPIYSHSKHLRLPETRAV
                     GQHAIIVLEGILLFSDKALREIMDIRIFMSTPLDVCLTRRLKRDVVERHRSFESVIHQ
                     YETTVRPMYMQFIEPSSRYADIIVPRGGENRIAIEMIQAKMRELLASHTRGS"
     gene            2438..3244
                     /locus_tag="VAH76_002446"
     CDS             2438..3244
                     /locus_tag="VAH76_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEO1387045.1"
                     /translation="MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFT
                     YQNEKLQSRVETIASEFNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAP
                     ADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAV
                     PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYA
                     NITPLQRNVTADEVGNTAAFLCSDLASGITGEVLHVDAGYHAVSAMSELG"
     gene            complement(3782..5656)
                     /locus_tag="VAH76_002447"
     CDS             complement(3782..5656)
                     /locus_tag="VAH76_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEO1387046.1"
                     /translation="MLQKLNEHIQGVVAWLVIILIAITFTLFGVDYYFQSRQISDAKV
                     IVNDKPITMQAFETNYRRTRAQQDLPQMTAADEKNLQNQVLNQMITNEVSIQAARKYG
                     FEVSPEQANAAIVQIPQFQEDGHFSAQRYQQALSGALFTPETFQNEVRQGMLLNQQRF
                     AFMGTSFALSDEIKRFVRLYMQTRDYDYLTVPSDRFEQQAKISEDDIKSYYNQHHKKF
                     MTSEQVTLDYVLLTMHDIKSQIKISPDEIKSYYEENKSNYLTPAQWRVAHILFAVPEN
                     ATKEEQDSIKQKADEVYSDLKKHPEQFDKYVASKSDDKLSIANKGILPWITGGQNEYG
                     RVLSNLTRPGEISTPVQTKYGYEIFKLIAYKPVTTKSLQEVENIIKDQLTSDMAQAKY
                     AQVLEQLTDLSYQTPDSLDPVLDSLKLKMQHTEPFSRDGGKQPITKNKQVIHAAFSTD
                     VLELGNNSEPIQIDNDSVIVLRVKKHIPSREQTLDEVRDQIKKILAKQYGDAKAKEIG
                     SSLLHPVEDKQQQELIANNQFEWHSVENASRDSDKANSLINDLAFNLLRPESRDGVIL
                     DNGDYVVVKLKRINDGKLSSLDQEQRDSLIQQIEASYGMMDYDLYVNNLLHHAKIERK
                     "
     gene            complement(5748..5824)
                     /locus_tag="VAH76_002448"
     tRNA            complement(5748..5824)
                     /locus_tag="VAH76_002448"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5788..5790),aa:Asp,seq:gtc)
     gene            complement(5831..5906)
                     /locus_tag="VAH76_002449"
     tRNA            complement(5831..5906)
                     /locus_tag="VAH76_002449"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5871..5873),aa:Val,seq:tac)
     gene            complement(5914..6192)
                     /locus_tag="VAH76_002450"
     CDS             complement(5914..6192)
                     /locus_tag="VAH76_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO1387047.1"
                     /translation="MNKSELVDAIASGSGLTKADASRVLETFMATVTDVLKKGDQVVI
                     PGFGSFSTGNRSARTGRNPQTGKTIQIKASRVAKFKAGKNLKEAVQQA"
     gene            complement(6321..8771)
                     /gene="lon"
                     /locus_tag="VAH76_002451"
     CDS             complement(6321..8771)
                     /gene="lon"
                     /locus_tag="VAH76_002451"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEO1387048.1"
                     /translation="MSNENEIISNETVKSSALPVLPLRDVVVYPHMVIPLFVGRGKSI
                     KALEAAMIDNKQIFLVAQRKSAHDDPGPEDIYQVGTVSSVLQLLKLPDGTVKVLVEGE
                     QRARVKEYTQDKGYLEATLEYIEEVGSTIQEQEIGILMRSLMSQFEQYIKLNKKIPPE
                     VLSPLAGIEEPGRLADTIAAHLTLKVDDKQELLETMDVGARLEKLMAAIENEIDLLHV
                     EKRVRGRVKRQMEKSQREYYLNEQIKAIQKELGEMGEEGNEIEQLENSIKKAGMPKEA
                     KEKSLAELHKLKMMSPMSAEATVIRNYLDWMLMVPWKKRTKIQFDLLKAEKLLDKEHH
                     GLEQVKERIIEYLAVQQRVKRLKGPILCLVGPPGVGKTSLGQSIANATGRTFIRIALG
                     GVRDEAEIRGHRRTYIGSMPGKIIQKLCKAGVKNPLIMLDEVDKMAMDFRGDPASALL
                     EVLDPEQNHTFSDHYLEVDYDLSDVMFIATANSLEIPAPLLDRMEVIRLPGYTEDEKV
                     SIAEKYLVPKQMLVNGLTNEEIHISEGAIRDIIRYYTREAGVRNLERDIASICRKVVK
                     EILSSKKGKKGKTISITVSNITKYLGVKKYRYGLAEQYDQIGQVTGLAWTSVGGELLT
                     IEASIMPGKGKVTHTGQLGEVMQESIHAAMTVVRSRAKAFGLPDDFYEKHDFHVHVPE
                     GATPKDGPSAGIGMCTVLVSIVTQIPVKADVAMTGEITLRGQVLPIGGLKEKLLAAHR
                     GGIKHVIIPEENVKDLEEIPDNVLRKLTIHPVNTIEQVLELALQRNPWVEEPTNIQPG
                     TILNKRSKKIKNNDLHAH"
BASE COUNT         2599 a         1877 c         1555 g         2833 t
ORIGIN      
        1 gatggtcata aaaatgaaaa aaataagctg cagtattctt cttttgatca actccttaat
       61 tagctacgct gattcagacc aacctaaatt aattttacaa atagttgttg accaattacg
      121 cggagatctg atttaccgtc atcataaaca gtttggacct tctggtttca attatttgct
      181 tgctcatggt ttggattatc ataacgcaca tcatcctcat gccaatacaa cgacctgcgc
      241 aggccatagc actattgcaa caggaagcta cccctcttta cacggcatag ttgataatga
      301 ttggtatgat agaaaaacaa gaaaactaaa gtactgtatg gaggatttac aagccaaaat
      361 tttacccact gtacacaccc atatagaaat ttcaggacgc tcaccgcgaa acctaaaacc
      421 atccacatta agcgatgaaa tcattttagc aaataaaggg aaagcctttg gtgtatcatt
      481 aaaagacagg gcagccatca ctctagctgg tcacgcagga aaagcgtttt ggtttgacaa
      541 aacgaatggt ggatttatta caagcactta ctactattcc agttatccac tatgggtaga
      601 aaactggaat aaacactatc aacccaaagc ctacgactgg aatctttctc atcctaaatc
      661 attctatcag aatgcaaata ctcttccatt tcgtcataac tatggcagct ttggtcaaac
      721 atttccccat catgtaacca atcctccatc tgaagaatat ttcaaatcgc tttccagaac
      781 accaaaggca gatgaattaa cagctgattt tgccaaacaa ctactggtta atgaaaaatt
      841 gggaaaaaca acaggccaaa cagattatct tgcaatcagt ttttctgctg tagatgctat
      901 tggtcaccaa tttgggccta atagtctgga agcagaagac aatttgatcg cccttgatga
      961 caccctaagc caacttttta aaaccattga taaagaggtt ggtcttaata ataccttaat
     1021 aatactcacc gcagatcatg gtgtcagcga tggtccatct taccttaaag cacataaaat
     1081 acctgaaatt aaacctgtta atatagaagc aacaggaaaa cacatcgaga atttattatt
     1141 gaaacgtttc aacctgcctg cgcaagcttt aatgtcaatt aatcctcctt ttatatacct
     1201 tgatcaccaa attataaaag agaaaaattt ggatattgca gaggtcagtt tctacctggc
     1261 agaagaaatc agtcatcttc cagggatatt caaagcctac cccttaccta ttcgtgatat
     1321 tgaaaaggat tggttaagcg caaaagttga ccggatgtct tacgcttacc gcgcaggcga
     1381 tgtttacatt gttcaaccgc catatcaaag ccacggtgca aaaagtgaag atcgagttgc
     1441 tcatggcagt ccctggcaat atgatactta tgtcccgcta ctgtttgtac accctgactt
     1501 caaaccaaaa cgtatattca ggcaagtaca cacaacggat attgcaccaa cattatcttc
     1561 cttattattg attaaacccc ctgccgcagc agtaggacag cctttagttg aagtagtcaa
     1621 tgcttttcat aagactggag agaatgattg aattccaact gaaaaaactt tactattcgt
     1681 acaaaatttt taatgaaaac attatgacaa agcaagcaat tattattggg atttccggcc
     1741 catcagcatc tggaaaaagt ttattagcaa atactatagt caatgaatta ggatccgagc
     1801 aggttgtcgt tatctctgaa gatgcctact ataaagataa tgggcatcta ccctttacag
     1861 agagagaaaa aataaattac gatcatcctg actcctttga tcatgctcta ctttatgagc
     1921 atcttcgtca gctacgagtt ggaaatacag tacaaattcc tatttattct cattccaaac
     1981 atctgcgttt acctgaaacc agagcagtgg gtcaacatgc cattattgta ctcgaaggaa
     2041 tactgctttt cagtgataaa gctctacgag aaattatgga tatacgtata ttcatgtcga
     2101 ctcctcttga cgtctgttta acgcggcgct taaaaagaga tgtagtagaa aggcatcgat
     2161 catttgaatc ggttattcat caatatgaga ctacagttcg ccctatgtac atgcaattta
     2221 ttgagccttc aagccgctac gctgatataa tagtacctcg cggcggtgaa aacagaatcg
     2281 ctatagagat gattcaagct aaaatgcggg aattattagc ttcacatact cgaggcagtt
     2341 gatgttttat taggctgttt aagagcaacc taattgatat tgtggtttta gtccaggatc
     2401 gaagtaacac tcacttcgcc ctgctaaatt aagaataatg ggaggagata ctattgtggg
     2461 atttttaact ggaaaaaaag cacttattgt tggattggca agcaacagat cgattgcata
     2521 tggcattgct aaagcattcc ataatcaagg cgctgagttg gcttttactt accaaaacga
     2581 aaaattacag tcacgtgttg aaacaatagc atcggaattt aattcaactc tagtttttcc
     2641 ttgtgatgtt gcaagtgatg aagaaataaa agccgtgttt gataatttgc gtaatcactg
     2701 ggataaatta gatatcctgg tccactctgt agcttatgct cctgctgatc agatcagtgg
     2761 tgattttgta gaatgtgcaa atagagaagg atttagaata gctcatgata ttagtgccta
     2821 tagcctcata ggtttgtctc aagccgcatt gcctatgatg ctagatactc agggttcaat
     2881 tttaacctta agctattatg gggcagaaaa agccgtacca aactataacg ttatgggtgt
     2941 tgcgaaggca agcttggaag cctctgtgcg ctatctggca gccagcttag gatccagagg
     3001 attaagaatt aatgccatat cagctggacc aattaaaact cttgccgctg ctggaattaa
     3061 agattttcgt aaaattcatg ctgcctatgc aaacattact ccattacaaa gaaatgttac
     3121 tgctgatgaa gtcggaaata ctgctgcctt cttatgctct gatctggcat ccgggattac
     3181 tggtgaagtg ctacatgttg atgcaggata tcacgcagta tcagcgatga gcgaactagg
     3241 ttagatatat attagtccgc tttaaattgc tgacttaatt cttagttgta cttaaaaaaa
     3301 ttcaataaag ctaaattata ttggggctga gcaaatagcg aggcccagac tactaaacgt
     3361 tttttataat ataacagaca aatttagatt tagcctatct aatctattga atcgacatga
     3421 aatagagcac ctcaatcaaa tgactcctgc gactccttca ttaacggatt attattaaga
     3481 gaataaacct caacttaaaa agaccatatt tagctgattt tgaatgaaaa taatccttca
     3541 aaagctcata taaccacctg cgctctattc aggatcattt tcgtccagcc tgaaccccaa
     3601 accaaccgct ataaaatatt tcaacgcgta agtctatgaa cagcttcagg cttattaatg
     3661 tgatatttat atcaggagca actttgaaaa agcctcacag agaaaaacta taggcaaaaa
     3721 ctgaagaatt ctcgatatct ggccatccct taatttcgta aggaacagcc attttcttat
     3781 cctatttcct ttctattttc gcatgatgaa gcaagttatt aacatataaa tcataatcca
     3841 tcattccata gctagcttct atttgctgta tcaagctatc tctttgctct tgatccagag
     3901 agcttaattt tccatcatta attcgtttta atttaacaac aacataatcg ccgttgtcta
     3961 aaattactcc gtctcggctt tctggacgta ataaattaaa tgctaaatca ttaattaaag
     4021 aatttgcttt atcactatcc ctggaagcat tctctacaga atgccattcg aattgattgt
     4081 tagcgattaa ttcctgctgt tgcttatctt caacggggtg aagcaaacta ctaccaattt
     4141 cttttgcttt agcatcccca tactgttttg caagtatttt tttaatctga tctcgcactt
     4201 catccaaggt ttgttctctt gaaggtatat gtttcttcac tcgtaaaact attacagagt
     4261 cattatcaat ctgtataggc tcgctattat tccctagttc taaaacatca gtgctgaatg
     4321 ctgcatggat aacctgctta tttttggtaa taggttgctt gccaccatca cgagaaaaag
     4381 gctctgtatg ttgcattttc agctttaatg aatccaaaac cggatcaagt gaatcaggag
     4441 tttgataact taaatctgtt aattgctcta aaacttgagc gtattttgcc tgagccatat
     4501 cgctggttag ttgatccttt ataatgtttt caacttcttg caaagattta gttgtaactg
     4561 gtttgtaagc aatcagctta aaaatttcat aaccatattt tgtttgtact ggtgtagaaa
     4621 tttcccctgg tctagttaaa ttggaaagta ctctgccata ttcattttgc ccgccagtaa
     4681 tccagggaag aataccctta ttggctatag ataacttgtc atcagatttg gaagcaacat
     4741 atttatcaaa ttgttctgga tgctttttta aatcgctata aacctcatcg gctttttgct
     4801 taattgaatc ttgttcttcc ttggtggcat tttctggaac tgcaaataaa atatgggcaa
     4861 cccgccattg agcaggagtt aggtagttac ttttgttttc ttcataatag cttttaattt
     4921 cgtcgggtga tatctttatt tgtgacttga tatcatgcat agtcaaaagc acataatcca
     4981 atgtaacttg ctctgaagtc ataaattttt tatggtgttg gttataataa ctcttgatat
     5041 catcttctga aatcttcgcc tgttgttcaa agcgatcaga aggtaccgtc aaataatcat
     5101 aatccctggt ctgcatgtac aatctgacaa aacgctttat ttcatctgat aaagcaaatg
     5161 atgttcccat aaacgcaaaa cgttgttgat taagcaacat gccttgcctt acttcattct
     5221 ggaaagtctc tggagtaaac aaagctccac tcaaagcttg ttgatatcgt tgtgctgaaa
     5281 aatgtccatc ctcctggaat tgaggtattt gcactatggc cgcattggct tgttctggac
     5341 taacctcaaa tccatatttc ctggcagcct gaatactcac ctcattcgtt atcatttgat
     5401 ttaatacttg attttgcaga tttttttcat cagcggcagt catctgtggt aaatcttgct
     5461 gagcacgcgt acgtctgtag ttcgtttcaa acgcttgcat ggtaattggt ttatcattaa
     5521 ctattacctt tgcatcggat atttgtcgag attgaaaata ataatcaaca ccaaaaagtg
     5581 taaaggtaat agcaattaat ataataacta accacgctac tacaccctga atgtgttcat
     5641 ttaacttctg caacatgtcc cgagatccat tttgatatta acaatgcttg atgaaaacga
     5701 ttctaccata agctgataaa aaaaacgcgc ctttcagcgc gtttatctgg cggagtggac
     5761 ggggctcgaa cccgcgaccc ccggcgtgac aggccggtat tctgaccaac tgaactacca
     5821 ctccatttct tggtgggtgc tgtagggatc gaacctacga ccctcgcctt gtaagggcga
     5881 tgctctccca gctgagctaa gcaccctgaa aacttaagct tgttgtaccg cttctttcag
     5941 gtttttacct gctttaaatt tggcaacccg tgacgcttta atttggattg tttttccagt
     6001 ctgtgggttt ctgccagtac gtgctgaacg attacctgtt gaaaaagaac caaaaccagg
     6061 tattacaact tgatcgcctt tctttaagac atcagttaca gtagccataa aagtttcaag
     6121 aactctgctt gcgtcagctt tggtcaaacc tgaaccgctt gctatagcat caactaattc
     6181 actcttattc atcttttccc ctttacagtt aatcatccat attccggagc tgatttaagc
     6241 agattcaact gctccagtca agttcgtaca tccttgcaga gcccgccagt agcgggccac
     6301 gtagagaaat atactctgta ttaatgtgcg tgtaaatcat tatttttaat tttttttgat
     6361 cttttattca atatagtgcc aggttgtata tttgttggct cttccaccca tggattgcgt
     6421 tgcaaagcca gttccaaaac ctgttcaatg gtgttaacag gatgaattgt cagcttgcgt
     6481 agcacattat cagggatttc ctcaaggtct tttacattct cctcgggaat aatgacatgc
     6541 ttgattccac cacgatgtgc agccagcaat ttttctttta atcctccaat tggtaaaact
     6601 tgcccacgca gagtaatttc acctgtcatg gcgacatctg ccttaactgg gatttgtgtt
     6661 actatggaga ctagtaccgt gcacattcct ataccagcgc ttgggccatc tttgggtgtt
     6721 gcaccttctg gtacatgaac atgaaaatca tgtttctcat aaaaatcatc tggcaagcca
     6781 aaagctttag cgcggcttct tactaccgtc atagctgcat ggatagattc ttgcattacc
     6841 tcaccaagct gaccagtatg agtaacttta cccttacctg gcataatgga tgcttctata
     6901 gtcagaagtt ctcctccaac acttgtccag gctaacccag tgacttgacc gatctgatcg
     6961 tattgttcag ctaatccata acgatatttt ttaacgccca aatacttagt aatgttgcta
     7021 acagttatac tgattgtttt tccttttttt ccctttttgc ttgagagtat ttctttaaca
     7081 acttttctgc atatactcgc tatatccctt tcaagattac gtactccagc ctctcgcgta
     7141 taatagcgga tgatatctct aatagcccct tcgctaatat ggatttcttc attagttaaa
     7201 ccatttacca gcatttgttt gggaaccaaa tatttttcag caatactgac tttttcgtcc
     7261 tcagtatatc caggcaagcg aataacttcc attctatcca ataacggggc tggaatttct
     7321 agcgagttgg cagttgcaat aaacataaca tcactcaagt cataatcaac ttccaggtaa
     7381 tgatcactaa acgtatggtt ctgttcagga tccaatactt ccagtaatgc cgatgccgga
     7441 tcaccgcgaa aatccattgc catcttatct acttcatcca gcatgataag aggattttta
     7501 actcctgcct tgcataattt ttggataatt ttacctggca tggaacctat atatgttctt
     7561 cgatgaccac gaatttctgc ttcatctctt accccaccca gagcgatgcg tatgaaagtt
     7621 cttccagtag cattggcgat agattgtcca agagaagttt tacctactcc cggaggacct
     7681 actagacaca atatcggtcc tttcaatctt tttactcgtt gttgtacagc aagatattcg
     7741 atgatacgct ctttaacctg ctccagacca tgatgctcct tatcgagcag cttttctgct
     7801 tttagtaaat caaactgaat tttggttcgc ttcttccatg gtaccatcag catccaatca
     7861 agataattac gaataacagt tgcttcagca gacatcggag acatcatttt taatttatgt
     7921 aactctgcta atgatttctc tttagcttct ttcggcattc cggccttttt gattgaattt
     7981 tccaattgtt caatctcatt gccttcctca cccatttccc ctaattcttt ttgaatagct
     8041 ttgatttgct cgtttagata gtattctctc tggctttttt ccatttgacg tttgactcga
     8101 cctcttacac gtttttccac atgtaacaga tcaatttcat tttctatcgc cgccattaat
     8161 ttctcaagcc gagctcctac atccatagtt tcaagcaact cttgcttatc atctacttta
     8221 agcgttaaat gtgcggcaat ggtatcggcc aagcgacctg gctcttctat tcctgccaat
     8281 ggtgacaaga cttctggtgg aattttctta ttaagcttta tatattgttc aaattgcgac
     8341 atcaaggacc gcattagaat tccaatttct tgctcttgaa ttgttgagcc aacttcctca
     8401 atgtattcaa gtgtcgcttc gaggtaacct ttatcctgag tatactcctt gacacgtgca
     8461 cgctgctcac cttcaacaag aacttttaca gttccgtcag gtaactttaa caattgcaaa
     8521 acactggata cagttcccac ttgatagata tcttctgggc caggatcatc atgcgccgat
     8581 ttacgctgcg caactaaaaa aatttgttta ttatcaatca tcgctgcttc aagagcttta
     8641 attgacttgc cacgcccaac aaagagaggt atcaccatat gcggatagac gactacgtcc
     8701 ctcaatggta atacaggcaa agcagaagat ttcacagtct catttgatat tatttcattt
     8761 tcattagaca taatgaccct tattcaaagg aaaatttctt ggttatagtt tcttatacta
     8821 ttacttacga gtatatagat attggtgctg gtgaacaatt cgta
//
LOCUS       DATNSN010000135         8843 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.135, whole genome shotgun sequence.
ACCESSION   DATNSN010000135 DATNSN010000000
VERSION     DATNSN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.135"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            50..484
                     /locus_tag="VAH76_002452"
     CDS             50..484
                     /locus_tag="VAH76_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEO1387049.1"
                     /translation="MKLWKVLCSFVIAVCYLPLAFAASQSPAGTWTTIDDKTGAKRAV
                     VNLKVSNGTLSGTIVKVYPQPGDTGICSKCPGAFKDKKIQGLTFVWGLKDEGNGVWSG
                     GSILDPKTGKIYKAKMTLQGNKLYVRGYFGVSALGRTQTWIR"
     gene            662..2044
                     /gene="dnaB"
                     /locus_tag="VAH76_002453"
     CDS             662..2044
                     /gene="dnaB"
                     /locus_tag="VAH76_002453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO1387050.1"
                     /translation="MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNK
                     LCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANN
                     TPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
                     QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVA
                     GRPSMGKTTLVMNMAEHAAIKAGKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGK
                     LDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKEHGSLGLIVVDYLQL
                     MKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRE
                     SGAIEQDADLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFED
                     LAFNGYQGVE"
     gene            2046..3119
                     /gene="alr"
                     /locus_tag="VAH76_002454"
     CDS             2046..3119
                     /gene="alr"
                     /locus_tag="VAH76_002454"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO1387051.1"
                     /translation="MSRPTRLVIEPSALLHNLSQIKHLAPGKKVIAMVKANAYGCGVR
                     EVAPVLDGRIEAFGVACLEEALAIRALGVETPCILFQGVFSSDELSVAVENDFACILH
                     HAQQLEWLIKTPLPYPIKIWVKVNTGMHRLGFKIHELQKVMGALQTCTWVDKNIGLMT
                     HLACADEPHRPENQQQISLFQEISIPGFRQRSIANSAAIISFPDSHADVVRPGIMLYG
                     VSPFANQNARDLSLIPVMRFMSAISAIHDNPSFAQVGYGGTWKSDKPSRIGIVAAGYG
                     DGYPRHISEKTPVWVRGREVSIVGRVSMDMLTIDLTEHPDVEIGDEVELWGTHVLVER
                     IAKSAGTVGYELLCQISERVRYK"
     gene            3192..3641
                     /locus_tag="VAH76_002455"
     CDS             3192..3641
                     /locus_tag="VAH76_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEO1387052.1"
                     /translation="MKKLIVASLSLTFMLGGCASMNKEGVGTITGGVVGGLLGSQFGG
                     GSGKVAAAGAGALLGAYLGGQIGKTMDKVDRLEMQRALETAPTGKAVAWSNPDTGYRY
                     TVQPTRTYYNAQQPCREYITKAMIGGKTQQIYGKACRQADGSWRVVN"
     gene            3947..4381
                     /locus_tag="VAH76_002456"
     CDS             3947..4381
                     /locus_tag="VAH76_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO1387053.1"
                     /translation="MAHLIHSVLPVPPRKIAYIHPDDSVKECIKQMVEKDIGALVVFD
                     NDAHLIGIVSERDILRCYFHKNLSLETAKVSDVVYNNVTILSPHDSVEKAMQVITETK
                     RRHVLIQEEGELLAILSIGDLLYHVLEDKLRVIEHLENYIHS"
     gene            4490..5677
                     /locus_tag="VAH76_002457"
     CDS             4490..5677
                     /locus_tag="VAH76_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387054.1"
                     /translation="MKTMKQMIRLTMLLLMLSSNLWAINSSSPWYTEEKGKGLVINVE
                     LYLSSTCPHCHKADEFFKEIEKHYTWLHVERYIIDKDKKALKRFGDLLTELNRYDFAV
                     PSIFFCNSRWVGFATAQTTGKDLLNGIEYCKEEIVKNGRLTPATESVLNRWGNASLFD
                     SNITGSPSTNTFIFVVALIDAISPCALFTITAFLGLLFMIEKRKLQFISGSVFILTVA
                     GVHYFQQVYPTLFFESLSWFRIPAALVGLFAFYFAGQYYKKHSIQPLFIVLAFLLALT
                     VPMFQQTCLMNWSYVFEQWLHNQNVSGVQMGLYQFAYQLIYIFPLIILMFLYWVLMKI
                     HFFKKFKTKLTTIGFLYILAIALLLIIYPYALSNLALSLFLIVSLGISGLLLNWFNAS
                     KMT"
     gene            5789..6061
                     /locus_tag="VAH76_002458"
     CDS             5789..6061
                     /locus_tag="VAH76_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEO1387055.1"
                     /translation="MDDNNSEFKHLFPIAVEVTEGKTYVWCGCGKSKTQPFCDKENCG
                     DKAVSFIAELTEDVYFCNCKQTKSPPFCDGSHAKILLEVVKKRQNK"
     gene            6081..7631
                     /locus_tag="VAH76_002459"
     CDS             6081..7631
                     /locus_tag="VAH76_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEO1387056.1"
                     /translation="MIDQELKKKIVNTISQYEHKCDKMQSQINTLRNVINQLIINPIG
                     INYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEILSKMQKKKAENSRLVNGLIKQGV
                     ESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEELPQIT
                     KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTII
                     DNLTELVVESFNVEQNRFKGFLQQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQ
                     DNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIREEEYAKQVSELQAR
                     LQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSRALSLAV
                     CDIDHFKRINDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKE
                     AFGILEKLRKLVEDCLFYYRDKKVDVTVSFGLTTVTEEDDIESLFMRADNAMYKAKNA
                     GKNRVEIL"
     gene            7783..8772
                     /gene="lipA"
                     /locus_tag="VAH76_002460"
     CDS             7783..8772
                     /gene="lipA"
                     /locus_tag="VAH76_002460"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEO1387057.1"
                     /translation="MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPK
                     WLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRAC
                     RFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
                     IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQ
                     TLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKN
                     HLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV"
BASE COUNT         2739 a         1472 c         1920 g         2712 t
ORIGIN      
        1 gtacagaatt tatacaagag gtatactgat tcaattacgg agattactta tgaaattatg
       61 gaaagtgtta tgtagttttg taattgctgt atgttattta ccattagcct ttgcagcatc
      121 acagtctcct gccggtacct ggacaaccat tgatgataaa accggagcta agagagcagt
      181 agttaacctt aaagtatcaa atggtacttt aagtggtact attgtgaagg tatatccaca
      241 accaggtgat accggaattt gttctaagtg ccctggcgca ttcaaggaca aaaaaattca
      301 agggttgact tttgtttggg gattaaaaga tgaaggcaac ggcgtctgga gtggtggctc
      361 tatcctcgat cccaaaacag gaaaaattta taaggctaag atgacactgc agggtaataa
      421 actgtatgtc agagggtatt ttggagtttc tgctctagga cgtactcaaa cctggattcg
      481 ataagtagta ctgaaggatg tttttatata taatgtggga tagtgctttc tcttgtcatt
      541 gaaagcagtt ggtactatcc cgcattttgt ttttagtgtt atgatcattt gcaattagat
      601 taggaatgtc tttaaataaa ttgtcttccc tataaataaa ttctcaaaaa atattattac
      661 catgcttgat ttgcaaatat ctaaaaaaac ggttgatcca ctaaaacgcc ccccgcattc
      721 tgtagaggct gagcaatcca ttattggtgg tttgatgctc gacaatcaag tctgggataa
      781 ggtaagcaat aaattatgcg aaaccgattt ttatcgtact gagcaccgaa ttctttttag
      841 agccatttct gctttagcca aaaaagatca accttttgat gtggtgacgc ttcttgatgc
      901 tttaaaaaca cgcaacgagt tagatgatgc tggtggagaa gcctatttat ttgagttggc
      961 taataacaca ccaagtgtag cgaatgtgac cgcttatgcg gatattgttc gggaaaaatc
     1021 agtccagagg caactgatag cggtagcaac agaaattgct gattccgctt ataatcctgc
     1081 tggaagagaa gtgcctgagc tattggatat ggctgaaacc aaagtatttg ctattggtga
     1141 acaaacagcg agtgacgggg gtccagaaaa tattaaatcc attttggtac gggcggtaga
     1201 gaaaatagat gccctctatc ataatggtga tgcgattaca ggccttgcga caggcttatc
     1261 cgatttggat gaaatgacat cgggtttaca gccttctgat ctgattatcg ttgctggccg
     1321 tccatcgatg ggaaaaacaa ctttggtgat gaatatggca gagcatgcgg caattaaagc
     1381 cggtaagcca gtattggttt tctctatgga aatgcctgct gattccctgg caatgagaat
     1441 gatgtcttcc ttggggcgaa tagatcagca tcggattcgt actggtaaat tagatgataa
     1501 tgactggcca cgagtgactt ccgcggtgca tatgctttct gaagctcctt tgtttataga
     1561 tgatactcca gctttaagtc ctggagaaat gagggcccgt gccagacgtt tagcaaagga
     1621 acatggttcg ttagggctta tcgttgtcga ttatttacaa ttaatgaaag tgcccgggtt
     1681 taatgcagac aatagaacag cggaaatctc cgaaatttct cggagtctta aatcgctggc
     1741 aaaagagtta caggttcccg taattgcttt atctcagctg aatagaagcc tcgagcagcg
     1801 agcagacaag cggccagtga tgtcagattt gcgagaatca ggagcaattg aacaggatgc
     1861 tgacttaatc tgttttattt atagagatga agtatataat gaggacagtc ctgataaagg
     1921 cactgcggaa atcattgttg ccaagcaaag aaatggaccc ataggcaaag tcagggtggc
     1981 ctttttgggt aagtacacac gatttgaaga tttggcattt aatggttatc aaggggtaga
     2041 atagggtgtc aagacctaca cgtttagtga tagagcccag tgctttattg cataatttat
     2101 ctcaaatcaa gcatttagct ccaggcaaaa aagttatcgc tatggtaaaa gccaatgctt
     2161 atggttgtgg agtcagggaa gttgcccctg tccttgatgg gcgtattgag gcttttggag
     2221 tagcctgcct tgaagaggct ctggctattc gagctttagg tgttgaaacc ccgtgcattc
     2281 tctttcaggg cgtctttagt tctgatgaat tgtcagtagc cgttgagaat gatttcgcct
     2341 gtattcttca tcacgcacag cagttggaat ggctaatcaa gactccgttg ccttacccga
     2401 taaaaatatg ggtgaaagta aacacaggca tgcacagact gggatttaaa atccatgaat
     2461 tgcaaaaagt gatgggggca ttgcaaacct gtacctgggt tgataaaaat atagggttaa
     2521 tgactcattt ggcttgcgca gatgagcctc atcgtcctga gaatcaacaa cagatctctt
     2581 tgtttcagga gatatccata cctggatttc gccaacgcag tatcgctaat tcggcggcaa
     2641 ttatttcatt ccctgattcc catgctgatg tggtgaggcc cgggattatg ttgtatggtg
     2701 tttccccatt tgccaatcaa aatgcccgtg atttaagttt aatccctgtc atgcgtttta
     2761 tgtcagccat cagcgcgatt catgataacc catcttttgc tcaggtaggc tatggtggaa
     2821 cctggaaaag cgataaacct tcgcgaattg gcattgttgc agcaggttat ggcgacgggt
     2881 acccacggca tatttccgag aaaacaccgg tttgggttag agggagggaa gtgtctattg
     2941 ttggcagagt ttctatggat atgctgacca tcgatttgac agagcaccct gatgttgaga
     3001 tcggcgatga agttgagctt tggggaacac atgttttagt agagcgcatt gccaaatcag
     3061 caggtacagt aggatacgaa ttattatgtc aaatttcgga gagagtacgt tacaaataag
     3121 ttatggataa taactgttcg tgatggtaga atgacaaccg attttgtaac tctttaaaca
     3181 ggtgaactgt aatgaaaaaa ttaattgttg catcattatc cctaacattc atgctaggtg
     3241 gctgtgcctc aatgaacaaa gaaggtgtag gtacaattac aggtggtgtg gtaggtggat
     3301 tacttggtag ccagtttggt ggtggttcag gtaaggttgc agctgcagga gcaggagcat
     3361 tattaggagc ttacctgggt ggtcaaattg gaaagacaat ggataaagta gaccgacttg
     3421 aaatgcaaag ggccctcgaa acagctccaa caggtaaagc agttgcatgg tcaaatccgg
     3481 atactggata ccgttatact gtccagccaa cgagaactta ttataatgct caacaacctt
     3541 gccgtgagta tattaccaaa gccatgattg gtggtaaaac ccaacaaatt tatggtaagg
     3601 cctgtcgcca agctgatggt tcctggcgtg ttgtgaatta attaattgat taagattgtt
     3661 ggggaaaacc caacaatctc attttattac tcaaattaat taagacagga cttacgaaaa
     3721 actcctatct ctatgttaaa aatattttta attcagtcat ttagtttatc taaattcctt
     3781 cattaaaatc attttttgcc ttgaccttgg agttttcaat atacaaatcc tacgataatc
     3841 ttttataaaa atctttcctg ccctcacaca atgagcttaa ccttccttgt tttgtgctat
     3901 atttatacag cgagttgcta atttagtatt aatcagggag agtgcaatgg ctcacttaat
     3961 tcatagcgta ctgccggtgc caccaaggaa aatcgcttat attcatcctg atgatagtgt
     4021 caaagaatgt attaaacaga tggttgaaaa agatataggt gcattagttg ttttcgacaa
     4081 tgatgcccac ttgattggta ttgtgagtga aagagacata cttcgctgtt attttcataa
     4141 gaatctaagt ttggaaactg caaaagtatc tgatgttgtt tataacaacg tcacgatctt
     4201 aagtccacat gattcggtag agaaagccat gcaagtgata actgaaacga aaagaagaca
     4261 tgtattaata caggaagaag gtgagttatt agctatattg tcaataggag acttgttgta
     4321 ccacgtactt gaagataaat tgcgagtgat tgagcatttg gaaaattata ttcactcctg
     4381 aaatataaac tcttatttca gatttatatt ttttgcttta aacgtgagtg tctggctatt
     4441 tcatacaatc gatcctaagt gctatagtat tcatttacaa agaaatataa tgaaaaccat
     4501 gaagcaaatg attcgattga caatgttact tctgatgctc agttctaatt tatgggcgat
     4561 aaattcttca tctccctggt atacagaaga aaaaggtaaa gggcttgtaa ttaacgttga
     4621 attgtatctt tcatctactt gtcctcattg tcataaggcc gatgaatttt tcaaagaaat
     4681 tgaaaaacat tatacctggt tgcatgttga gcgttatatt attgataaag ataaaaaggc
     4741 tttgaaacgc tttggcgatt tattgacaga acttaatcga tatgattttg ccgttccatc
     4801 catatttttt tgtaattccc gttgggttgg gtttgctact gcacagacaa ccggaaagga
     4861 tctgttaaac ggcatagaat attgtaaaga agaaattgtg aaaaatggaa gattgactcc
     4921 tgcgaccgag agtgttttaa acagatgggg gaatgcgagt ttgtttgact ccaatatcac
     4981 aggaagccct tccacaaata catttatttt tgttgtagcc ttaatcgatg caatcagtcc
     5041 atgtgcttta tttacgatta ctgcattttt aggtctttta ttcatgattg aaaagcgaaa
     5101 gctgcaattt attagcggtt ccgtgtttat tttaactgtt gccggtgttc attattttca
     5161 acaggtttat cctaccctgt tttttgagtc gctttcatgg ttccgtattc cggctgcatt
     5221 ggtaggcctt tttgcttttt atttcgctgg ccaatattat aaaaagcatt ctatccaacc
     5281 attatttatt gttttggctt ttttattggc ccttacagtg ccaatgtttc aacaaacctg
     5341 tttgatgaat tggtcttatg tgtttgaaca atggttacat aaccaaaatg tatcaggagt
     5401 tcagatgggg ctatatcaat ttgcttatca gcttatctat atctttcctt taattatcct
     5461 gatgtttctc tattgggttt taatgaaaat tcattttttc aaaaaattta agacaaaatt
     5521 aactactata ggttttttat atattttagc tatcgcactc ttattaatca tttatcccta
     5581 tgccttgtct aatttggctt tatcattatt tttaattgtc tcattgggga tctccggtct
     5641 gttactgaat tggtttaatg cttctaaaat gacatgaggg ctcaatcggc ataaaataat
     5701 tataaattct gaatatcttt cattttatgg catacaatta aaaatgagcc aggcttagaa
     5761 gctgatcgct tatggagaaa ttctcatcat ggatgataat aattcagagt ttaagcattt
     5821 atttccaata gctgtggagg taacagaagg aaagacttac gtatggtgtg gttgtggcaa
     5881 aagcaagacc cagcctttct gtgataaaga gaattgcggg gataaagcag tctcttttat
     5941 agcagagttg actgaagacg tttatttttg caattgcaaa caaaccaaaa gccctccttt
     6001 ttgtgatgga tcgcatgcaa agattttgct tgaagtggtt aaaaaaaggc aaaacaaatg
     6061 aacagggaag gaagtcttaa atgattgatc aagaattaaa gaaaaaaatt gttaacacaa
     6121 ttagccaata tgagcacaaa tgtgacaaga tgcaaagtca aattaatacg ctcagaaatg
     6181 tgattaatca attgattatt aatccgattg gaattaatta tgagttagat gatcgccttt
     6241 cgcaattaag aaattattta gatactgaat ttgaacctgc tgtcatagaa aaaaaagtga
     6301 aacgtttggc agaaatactt tccaaaatgc aaaagaaaaa agcggaaaat tctcgtttag
     6361 tcaatggatt aatcaaacag ggagtggagt ccattggtcg cattgccaat aaatctcagg
     6421 ataagagggc agttacaaaa ttacaaaaaa tgctggatac cgagatggaa agccatgcga
     6481 ttttgattcg ttttaatcaa gtcttaactc aatgtatttc ttctgtgatt aaggagatgg
     6541 aggagttacc acaaattaca aaagtcgata ccccccaaaa aagttatcag tctgagatta
     6601 gtttaaaagt aaatgacagc ttgcaacaat tacttaacca tttatctatc cctcaggact
     6661 tggattccaa gaggggagtc ataaaaacaa atttggaaca tgcactcact gatgaagagc
     6721 ttagcactat tattgacaat ttgacggaat tagtcgttga gtcatttaat gttgagcaga
     6781 atcgattcaa agggtttctt caacagttaa ccgatcagct caatgatttt gaaatctatt
     6841 taagagtatc aggggatgct cgcaaaaagg caagtgaaga cagtcgttta ttagagtgtg
     6901 ggattcagga taatcttgat caaatcaaga atcatttgga tacctctaaa acgattgagg
     6961 aattatcctc aaaggttagc caaaatataa aaatgattgg tgacaggtta aaagaatatc
     7021 ggcacaatga aaaaatccga gaggaagaat atgcaaagca agtatccgag ttgcaagcta
     7081 gattacagga atcagagcaa aatgcggaag aaataaaaaa tttgcttgct tttcagaaat
     7141 acagaattaa ccacgacagc ttaacaggct tgccaaatcg ggaatcttat gatgagcata
     7201 tcctggattc attccagcgc tggaaacgaa gttcaagagc attgtctctt gctgtgtgtg
     7261 acattgatca ttttaaacgt attaatgata attttggcca tcttgcagga gataaggttc
     7321 ttaaaaaggt agctatgatt tttaaatcat cgatcaggac ggttgatttt atcgctcgaa
     7381 taggtggtga ggaatttgtg tttatctttg aacaaaccag cagcaaggaa gcttttggaa
     7441 ttttggagaa attacgtaaa ttagttgaag attgtttgtt ttattatcga gacaaaaaag
     7501 tcgatgttac tgtttctttt gggctaacta cggtaaccga agaagatgat attgaatctt
     7561 tatttatgcg ggcagacaat gccatgtata aagctaaaaa tgcaggaaag aacagggtag
     7621 aaatattata gtggaatata tttccaacaa tttttaatta tactattttc ggagtaaatc
     7681 catggtaaca gatgattttc ctctgataac gatttttaaa tgaacttgat cttatctttg
     7741 tgatttggga tcatcataac tctttgtatt tcggatggcg tcatgggcaa attgatcgat
     7801 attccaatcg ttgtggaaag tggacaaaaa tataagacat ctcaaggtgt gacagctatt
     7861 aaagatggaa taaaatcatc aggtcaagat catgaacgac tgccaaaacc gaagtggcta
     7921 cgcatagtca atcatacaac tccagcctat agccaagtta aagagcaagt gcaaaaacat
     7981 cgtttggcaa cagtttgtga agaagcgaaa tgccccaaca tctcagaatg ttggtctcat
     8041 ggaactgcaa ctattatgtt aatgggagca gtatgtaccc gggcttgccg attttgttct
     8101 gttgatacag gaaatccgca tggttggctt gatgcagaag aacctgagaa taccgcggaa
     8161 actgtggctt taatgaatct ggattatgtg gttttaactt cagttaaccg tgatgattta
     8221 cctgatgggg gggcgaatca ttatgccaaa accattagag caatcaaaaa acgatctccc
     8281 cgaaccaaag tcgaagcgct aactcccgat tttcagggaa gtgaaagaga tgttgctgta
     8341 ttgttagata gtggcgttga tgtgtttgcg caaaatgtag aaacggttga gcgcctgaca
     8401 cacccggtaa gagataatcg ggcgggttat cagcaaacct tgaatgtact ggcttttgcc
     8461 aagaaatatc gaccggatgt tctgaccaaa accagtttga tgttgggatt aggtgaaacc
     8521 gatgaggaaa ttatacagac tatggatgat ctccgaactc atcacgtgga tattttgact
     8581 ttgggacaat atttgcaacc aactaaaaat catttaccta tagcacgtta cgtaactcct
     8641 gaaacttttt ctgaactaag gcaaattggt ctaaaaaaag gcttttttga agtggcttca
     8701 ggcccattag ttcgatcaag ctatagggct gaccgagtgt tcaaacggga taatttaggg
     8761 cttgatgttt gaagttagtg aacagcttgt gtttttcgaa ttggctataa ataattttat
     8821 tattagttaa agaaattaga ttt
//
LOCUS       DATNSN010000136         8843 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.136, whole genome shotgun sequence.
ACCESSION   DATNSN010000136 DATNSN010000000
VERSION     DATNSN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.136"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(170..1519)
                     /locus_tag="VAH76_002461"
     CDS             complement(170..1519)
                     /locus_tag="VAH76_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0634 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387058.1"
                     /translation="METNMTFVLKEFDVLKSHFNDTVKIILQREKKDKIEDLPNPRKE
                     ELQFLTAVLNQLEAKIDELKPRSLASYVHVFYGAMLLVCKDIENNLRVMEKKENSLLF
                     TRLMDGMGITDENMPTSEQNIMFYRGLNKFLNFIYENNDSRKGLKKEHFLQILSLKKI
                     YSLAKLSYEQEEAAENSALAKLTSDGKTKANANSFHVEKQIDPSIVEQFKSWDEMKGA
                     LHQLILDELSDKNVAKISALSQARSAQLKFLQTMAEQLDKIPNQSLESSEKMAILAGA
                     MYIVRGQIAQEYGKDPLSNDKISATVIHTGLSTILHANADCCEDKEVLIAAANKFIRH
                     MVIERPEQSNKKITKESIRENNMFSDIAGFQLVSVLTLIQNMIKICRTDAIEACVTKR
                     KEELEALKPKKEGYSIASSVTGYVGSWFKKAPTTSEEDEEDDLKDQNTAEETSKATI"
     gene            1813..3294
                     /locus_tag="VAH76_002462"
     CDS             1813..3294
                     /locus_tag="VAH76_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEO1387059.1"
                     /translation="MSFQIDKEIQKKFFNYTLFALVFLLICRLLANYFFPLFDTTESR
                     YAEIARKMLETGEWVTLQQDYGVPFWAKPPLSTWLSACFMQLLGVNEFAVRLPGLLLS
                     IGVLWLIWGLAKKQSGSMAALFSLLILAGSLYFFLDAGTVMTDPALLFCITLASVSFW
                     HAMVYESKIWSYAFFAGLGLGLLAKGPIAVVLVGMTVFFWVLLRNEWLNLWKRLPWFK
                     GTFLLLLIAMPWYVLAELKTPGFLNYFILGEHFHRFLTPGWSGDKYGIVHHVPKGTIW
                     LYAMAGIFPWNIIAGSWLVKHGKMLPSLCQSENGWLSYLTLWMITPLLFFTFASNIIY
                     PYVFPSLPAFALLFAEILNRLNLGLKHLKWVSISSSLCGVLFLVVAFVLSVKPDFVPN
                     THKPIIAAWLNQKPTTGSYLVYWGYKTDFSAEFYSGGQVKSVKNPNDLCKLLSNRLEN
                     YLVINSNETQQIPGEVLAKLTPIKTFRVHRDNLILFHSPVMTC"
     gene            3483..4112
                     /locus_tag="VAH76_002463"
     CDS             3483..4112
                     /locus_tag="VAH76_002463"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO1387060.1"
                     /translation="MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRY
                     QYGTISSRIGLSEQAYAFNNTDNLYVLTQELQLQAPKYSCVLIDEAQFLTREQVYQLT
                     EITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDE
                     NGQAVVEGKQVLIGGNESYVATCRLHYKRREAGMTFSRNKLFNKDTNTF"
     gene            4160..5431
                     /locus_tag="VAH76_002464"
     CDS             4160..5431
                     /locus_tag="VAH76_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387061.1"
                     /translation="MVSHSVAVESRTFVPRGDFMAWVVCLSAGLFFLYEFFQLNIFDV
                     INQSLREDFHIDATQLSWMSSTYLWADILFLLPAGLILDRFSTRKVILTAMFVCVVGT
                     IGFAITESFFLASFFHFLSGIGNAFCFLSCVVLVSHWFPPRRQALVIGSLVTMAFIGG
                     MMAHTPFAYLNELFGWRRALLIDGVVGAFLILWIYMIVRDRPEESPAHKLSNEGQILS
                     SFMKALSNKQNWLAGLYTSLLNLPIMVLCALWGASYLQVAHHLSDIAASNVVSLIFMG
                     SVVGCPLVGWLSDTQGRRKPLMIFGAVATLITTIPLLINVVLTQMSLSILFFALGLFT
                     STQVISYPLVAESNQAENTGAATGIASVIIMGGGGVAQVLFGWLMTHHAGTNVTAYTV
                     SDFQFAMWMFPVAAIAGLVAVLMTRETYCKR"
     gene            5446..6729
                     /locus_tag="VAH76_002465"
     CDS             5446..6729
                     /locus_tag="VAH76_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387062.1"
                     /translation="MQVVDEFKGYDIKASLMPWLVCFAATMFFFYEFIQGNMFASIAA
                     NIMQDFHIQADKMAYLSSIYYLANVIFLFIAGMVLDRFSIKNTILIAMFLCVISTFIL
                     SYSDSFYVALFCRFVTGIGSAFCFLGPVRLASHWFPPQRMALVTGAIVTVAMTGGMLA
                     QYPLTKLVLYVGWRQAVQDVGILGLAMLVLMFFWIKERPQVAVKKEGKPINVFVAARK
                     AYLNAQNLRAALYTSLMNMAIAVFGAMMGTLYLEQRLGISPDQAAMINGMLFFGAIIG
                     SPLMGWISDKVGLRVLPMKGGVLASLFILLGVLYLPVSVTMMSVLFFLLGLFTSAQVI
                     SYALVAESSPPMMTATAVSVVSILTQGGYLIYQNLFSTLLTNYGDVRLINGVPVYLLE
                     AYQHAAIILPIGLLIALILLFGLKETRCQQTEHTV"
     gene            6726..7949
                     /locus_tag="VAH76_002466"
     CDS             6726..7949
                     /locus_tag="VAH76_002466"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO1387063.1"
                     /translation="MTGRVCVLVMDSFGIGASLDAARYGDAGANTLVHIYEACMRGEC
                     NISGVRQGSLRLPNLAGKGLYHAAMASSGLPFIDLATLAIPSGYYGYAVEQSLGKDTP
                     SGHWEMAGVPVTFEWGYFPDKPYCFPKELISEFIKQCNLPGVLGEKHASGTIIIDELG
                     EEHIRTGKPIVYTSADSVFQIAAHEEAFGLQRLYDICKIARNLVDKYQIGRVIARPFT
                     GKPGSFKRTGNRKDYATPPPEKTLLDFLKEDGREVIAIGKIADIYAHQGVTQEIKADG
                     NMALFDATLSAMKTAPQGSLVFTNFVDFDSSYGHRRDIAGYAHALEQFDARLPELEVL
                     LQPNDMVFIAADHGCDPTFPGSDHTREHIPVLVFGPQVNSKFIGRRDCFADIGQSIAE
                     HLQLSSPLAHGVSFL"
     gene            8070..>8843
                     /locus_tag="VAH76_002467"
     CDS             8070..>8843
                     /locus_tag="VAH76_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEO1387064.1"
                     /translation="MQVESILPEILKGTIHKARINSLTSLVKGIVCHKKLKLSELGRH
                     LHLTSERSGIRIVDRVLGNTYYQERSIELYSCIAKTVIGDNQTPDILIDWSVIPNSQR
                     CTNEKEEYQVLRASFAATGRGITLYEEVHPRAKLGNEQVQSNFLMNLATILPFGCKPS
                     IITDAGFKIPWFKMVLKLGWNYIGRVRGEVQFNEDGEFKPISHLFPRASTKALYVGNL
                     VLAKYNPFKTNFYLYTHKLKGRKKTNRNGTFAKDKESIVH"
BASE COUNT         2405 a         1596 c         1892 g         2950 t
ORIGIN      
        1 ttaatatttg taaggtcaat tgagaaaaat attgtcacta ttgtaacaaa aattacgatg
       61 aatagcttat atttaaaagt ttattagaat aacaaaagct aaataaacag tcttattttg
      121 agaataagac tgtttatgaa tgaaaatcga cgttaattgt tcaaattaat tatattgtcg
      181 ctttactagt ttcttctgca gtgttttgat cttttaagtc atcctcttcg tcttcttcag
      241 acgtagttgg tgctttttta aaccagctac caacatatcc agtgacggaa ctcgcaatgg
      301 aataaccctc ttttttgggt tttaatgctt ccagctcttc cttacgtttg gtgacgcaag
      361 cttcaatggc atcagtacga catattttta tcatgttttg tattaacgtc aaaacagaaa
      421 ctaattggaa tccggctatg tctgaaaaca tgttgttttc tcgaatggat tccttggtga
      481 tttttttatt tgactgttca ggacgttcaa tgaccatatg acgaatgaac ttattggcag
      541 ccgcaatcag tacttcctta tcttcacagc aatcggcatt agcatgaagt atagtgctta
      601 aaccagtatg aatcacagta gcgctgattt tatcgttgct taatggatct tttccatatt
      661 cctgggctat ttggccacga acgatataca tcgctccagc aagaattgcc attttttcag
      721 acgactcaag agattggtta gggatcttgt ccagttgttc tgccattgtt tgtaaaaatt
      781 tcagttgtgc tgagcgggcc tgacttaaag cagaaatttt agccacgttc ttatcagaaa
      841 gttcatcaag aattaattga tgaagagcgc ctttcatctc atcccaggat ttgaattgct
      901 caacaatgga tgggtcgatt tgtttttcca catggaagct attcgcattg gctttggttt
      961 ttccatcgga agttaatttt gccaaagcac tattttcggc tgcttcttct tgctcataac
     1021 tcaatttggc tagagagtat atctttttta gcgaaaggat ttgcaggaaa tgctcttttt
     1081 ttaagccttt acgagaatca ttgttttcat aaatgaaatt taagaattta tttagtcctc
     1141 tgtaaaacat aatgttttgc tcagaggtcg gcatgttttc atcagtaata cccattccat
     1201 ccatcaagcg agtaaacagt aagctgtttt ctttcttttc catcacccgc agattatttt
     1261 caatgtcttt gcaaactagc agcatagcgc cataaaacac atggacatag ctggccaatg
     1321 aacgtggctt cagttcatct atttttgctt ccaattgatt gagaacagca gtcagaaatt
     1381 gaagctcttc ttttctgggg ttaggaaggt cttcaatttt gtcttttttt tcgcgttgaa
     1441 ggatgatttt aacggtgtca ttaaaatgac tttttagtac atcaaattcc tttaaaacaa
     1501 acgtcatgtt agtctccaaa aatccaagta agattaatgt aagaaatact tacagaggcc
     1561 agtgtaaact aaacttaagc tgacgtcatg tattttcatt atacaattta aaaatatata
     1621 ataatcattt taaaattaag aaaccgagct tggacgtgaa ggtcaatcaa aaaacaggaa
     1681 acgcaattta gggaataata ggctttatgg actttattcc cgggagcaat ctgtccgtgg
     1741 taaccacagg cacactctaa tactccactg gatggtaccc ggatttagtg gtttaaataa
     1801 cagaggtata ttttgagttt tcaaatagat aaagagatcc agaaaaaatt ctttaattac
     1861 accctgtttg cattggtttt tctgctgata tgtcgattgc ttgccaatta tttttttccc
     1921 ttgtttgata caaccgaatc ccgctacgct gaaattgcac gcaaaatgct ggaaacaggt
     1981 gagtgggtga ctttgcagca ggattatggt gtgccttttt gggctaaacc acctttgtcc
     2041 acttggttgt ctgcttgttt tatgcaacta ttgggtgtca atgaatttgc agtccgcttg
     2101 ccgggtcttt tgctgtctat tggtgtttta tggctgattt ggggactggc aaaaaaacaa
     2161 agtggctcaa tggctgcact tttttcctta ttaatactgg cggggtcgct ttattttttc
     2221 cttgatgcag ggacagtgat gactgacccc gcattacttt tttgtatcac gcttgcaagt
     2281 gtctcttttt ggcatgctat ggtttatgaa agcaaaatat ggtcttatgc atttttcgca
     2341 ggattgggcc tgggtttatt ggcaaaaggc ccaattgctg tggtactggt tggaatgact
     2401 gtatttttct gggttctctt acgtaatgaa tggcttaatc tatggaaaag attaccgtgg
     2461 tttaaaggta catttcttct tttgttaatt gctatgccct ggtatgttct ggctgaatta
     2521 aaaaccccgg gatttttaaa ttattttatc ctgggagagc atttccatcg atttttaact
     2581 ccgggctggt ccggtgataa gtacgggata gtacaccatg tacctaaagg aacgatatgg
     2641 ttatatgcta tggcaggcat ctttccatgg aatattatag caggcagctg gctggtgaag
     2701 catggaaaaa tgctgccctc tctatgccaa agtgaaaacg gatggttaag ttatttaact
     2761 ctttggatga ttaccccact gctttttttc acttttgcca gcaatatcat atacccctat
     2821 gtgtttcctt ctctacccgc atttgctttg ctttttgctg aaattttgaa tcgcttgaat
     2881 ttaggcctga aacatttgaa atgggtctct atctcatcat cactctgtgg agttttattt
     2941 ttggtagtag cctttgtact tagtgtaaaa ccagactttg tacctaatac acataagccg
     3001 attattgctg catggctgaa tcagaagcca actacaggca gttaccttgt atattgggga
     3061 tataagacgg atttctctgc tgaattttac tcgggaggcc aagtgaaatc ggttaaaaat
     3121 ccgaacgact tatgcaaact actttctaac cggttggaga actatttggt tatcaacagt
     3181 aatgaaactc agcagattcc cggagaagta cttgcaaaat tgacacccat caagacattc
     3241 agagttcatc gggataactt gatattgttc cattctcctg taatgacttg ttgagtagtg
     3301 gtttccaatt ccatctcata gtaaagtaac aggacttacg aaccgccaat ctctttgtta
     3361 aaaaaatttt taattcggtc atttagttta tctaaactcc ctcattaaaa actttttttg
     3421 ccttgacctt ggggtttttc ataagtcctg agtaaaacaa acgaggcggg aaatagaaat
     3481 ctatggcaaa attatatttc tactatgcag cgatgaatgc aggtaaaagc acagtgttac
     3541 tgcagtccag ttataattac agagagcgag gtatgcaaac tctgttattt accccagcca
     3601 ttgatacacg ttaccaatat ggaactatca gttcacgaat cggtttatct gagcaggctt
     3661 atgcgtttaa caatacggat aatctctatg ttttgactca ggaattgcaa ctacaagccc
     3721 caaaatactc gtgtgttctg attgacgagg ctcaattttt gactcgagag caagtatatc
     3781 aattaactga aataaccgat caaatgtcta tccctgtatt ggcttatggc ttgcgtactg
     3841 actttagagg tgaattattc ccagggagtc aatttttact ggcatgggca gatgagttaa
     3901 tcgaattaaa gacaatttgt cattgtggcc gtaaagcgat tatgaatatg agaattgatg
     3961 aaaatgggca agctgttgtt gagggtaagc aggtattgat aggtggtaat gaatcctatg
     4021 ttgctacctg tcggttgcat tataaacgaa gagaagcagg gatgacgttt tcaagaaata
     4081 aattgttcaa taaagatacc aatacttttt aagttttgat ataatttgct gcaattttca
     4141 gaccttaaat aaaggagaga tggtgtcgca ttctgtcgca gtggaatcga gaacttttgt
     4201 tcctcgtggt gactttatgg cttgggtagt gtgtctttct gcgggtcttt tctttttata
     4261 cgagtttttt caactcaata tttttgatgt tattaatcag tcacttcgag aagattttca
     4321 tatagatgcc acccaattaa gctggatgtc gagcacttac ttatgggctg atatcctttt
     4381 tcttttacca gctggtctta ttctcgacag gttttcaacc agaaaagtta ttctgacagc
     4441 gatgtttgtt tgtgttgttg gaaccatagg gtttgcaatt acagaatcat tttttctcgc
     4501 ctcgtttttc catttcctct caggaatagg taatgccttc tgttttttat cttgtgttgt
     4561 tctggtctct cattggtttc ctcccagaag gcaggcgtta gtcattggtt cattagtgac
     4621 tatggccttt attggtggca tgatggcaca cacacctttt gcttatttaa atgagctgtt
     4681 tggctggcga cgtgcattgt taattgacgg ggttgtaggc gcttttttaa tattatggat
     4741 atacatgatt gtacgagaca gacctgaaga gtcgccagca cataagttaa gcaatgaagg
     4801 acaaatacta tcgagtttca tgaaagcttt gtccaataaa caaaattggt tggcaggctt
     4861 gtatacctct ttacttaatt tacctattat ggtcttgtgt gccctatggg gagcgagcta
     4921 cctgcaagtg gctcatcatt tgtctgatat tgcggcaagt aatgttgtca gtcttatctt
     4981 catgggtagc gttgtcggtt gcccattggt tggttggtta tcggataccc aagggcgtcg
     5041 taaaccttta atgattttcg gagcggtagc tactttaatt actactattc ctctattgat
     5101 caatgtggtt ctgactcaaa tgagtctcag tatcctcttt tttgctttgg gattgtttac
     5161 cagtacccaa gtgatttctt accccttggt tgctgaaagc aatcaggcag aaaatactgg
     5221 tgctgcaact ggaattgcct ctgtcattat tatgggtggg ggcggcgttg ctcaagttct
     5281 gtttggttgg cttatgacac atcacgcggg aacaaatgtt acagcgtata cggttagcga
     5341 ttttcaattt gccatgtgga tgttccccgt tgccgcaatc gcaggtctag ttgctgtgct
     5401 catgactcgt gaaacatact gcaagagata gagagaggag aaataatgca ggtagtggat
     5461 gaatttaagg gatacgatat caaagcatcg ttaatgccct ggctggtttg ttttgcagca
     5521 accatgtttt tcttttacga atttattcag ggaaatatgt ttgcttctat tgcagcaaac
     5581 atcatgcagg attttcatat tcaagccgat aaaatggctt atttatcgag catctattac
     5641 ttggcaaatg tcattttttt gtttatcgcc ggtatggttt tggatcggtt ttcaattaaa
     5701 aacaccattt taattgctat gtttctctgt gtgatcagta cctttatcct ttcttattca
     5761 gattcttttt acgtggcttt attttgcagg tttgtaactg gcatagggag cgcattttgc
     5821 tttttaggtc ccgtccgttt ggcatctcat tggtttcctc ctcagcgtat ggcattagtg
     5881 actggcgcta ttgtaaccgt cgcaatgaca ggaggaatgc tggcgcaata tcctttaacc
     5941 aaactcgttt tatatgttgg ttggcgccag gcagttcagg acgtagggat attgggtttg
     6001 gccatgttag ttcttatgtt tttttggatt aaagaaaggc ctcaagtcgc agtcaagaaa
     6061 gaaggaaaac ctatcaatgt gtttgtggcg gcaagaaaag cctacttaaa tgctcaaaat
     6121 ttgcgtgctg ccttatacac cagcctaatg aatatggcca ttgcggtatt tggcgccatg
     6181 atggggactt tgtatcttga acagagattg ggtattagcc ctgaccaggc tgcaatgatt
     6241 aatggtatgc tattttttgg agccattata ggatcacctt tgatggggtg gatatctgat
     6301 aaggtaggat tgcgtgtgct gcccatgaaa ggaggtgttc ttgcctcttt gtttattttg
     6361 ctgggagtac tttatttacc tgtttcagta actatgatgt ccgtattgtt ctttctattg
     6421 ggattattta cctcagctca agttatcagc tatgctttgg tggctgaaag cagccctccc
     6481 atgatgactg caacagccgt cagtgttgtt tccatattaa ctcaaggcgg atatcttatt
     6541 tatcaaaact tgttcagtac tctgttgacg aattatggtg acgttcgttt gataaatggt
     6601 gtgccagtct atttgcttga agcttatcaa catgcagcaa ttattttacc tatagggtta
     6661 ttgatcgcat taattttgtt attcggatta aaagaaactc gctgccaaca aactgagcat
     6721 acagtatgac cgggcgagtt tgtgtattgg taatggattc ttttggaatc ggagccagcc
     6781 ttgatgccgc tcgttatgga gatgccggag ctaacacctt ggttcacatt tatgaagcct
     6841 gtatgcgagg cgaatgtaat attagtgggg ttcgtcaagg ttctttaagg cttccaaatc
     6901 ttgcaggcaa ggggctttac catgctgcca tggccagttc aggattacct tttattgatt
     6961 tggctacttt agctattcct tctggatatt atggatatgc ggtagagcaa agcttgggca
     7021 aggatactcc gagtggtcat tgggaaatgg ctggagtgcc tgtgactttt gagtggggat
     7081 atttcccgga taaaccttac tgtttcccta aagaattaat ttcagaattc attaagcagt
     7141 gtaatttgcc aggagttttg ggagaaaagc atgcttcggg aaccatcatt atagacgagt
     7201 tgggtgagga gcatattcgt accggtaagc cgatcgtata cacttctgcc gatagcgtat
     7261 ttcaaattgc cgcacatgaa gaggcatttg gtttacaacg tctctatgat atctgtaaaa
     7321 ttgctcgaaa tttagtagat aaatatcaaa ttggtcgagt gattgctcgt ccttttacag
     7381 gtaagccggg ctcatttaag agaacaggta atagaaaaga ttacgctaca cctcctccgg
     7441 aaaaaacatt gcttgatttt ctaaaggaag atgggcggga agtgattgct ataggtaaga
     7501 ttgcagacat ctatgctcat caaggggtta cgcaagaaat caaagcagat ggcaatatgg
     7561 ctttgtttga tgccacttta tccgcaatga aaacagcccc gcaaggtagc ttggtattta
     7621 ccaattttgt tgattttgat tcttcttatg ggcataggcg tgatatagct ggttatgcgc
     7681 atgcgctaga gcaattcgat gcgcgtctgc cagagttgga ggttctctta caacccaatg
     7741 acatggtgtt tatcgcagca gatcacggat gtgatcctac attccctgga tctgatcata
     7801 cccgcgagca tattccagta ttggtgtttg gccctcaggt aaatagcaaa tttattggac
     7861 ggagagattg tttcgcagat attggtcaga gtattgcgga gcatttgcaa ttatcctcgc
     7921 cactcgctca tggtgtttct tttttataat tttatatcca ctcccttacg gctgaggtat
     7981 cgactcattt ttaagataaa agaaagaaga caagcatagc aaaaccatga tctaatagtt
     8041 tcgcgaaaaa gaactagata ggttgagcta tgcaagtgga atccatttta cctgaaatac
     8101 ttaaaggtac aattcataaa gcaagaataa actctttaac tagcctggtt aaaggcatag
     8161 tttgtcataa aaaattaaaa ttaagcgagc taggtcggca tttacacctc acaagcgaac
     8221 gttcaggaat aaggattgta gatagggtat tagggaatac gtattatcaa gagcgctcaa
     8281 ttgagctcta tagttgtata gctaagactg ttattggtga caatcaaaca cctgacattt
     8341 taattgattg gtcagtgata ccaaatagcc agcgctgtac taatgaaaaa gaagaatatc
     8401 aggttctcag agcttctttt gccgcaacag gtcgaggtat taccctttat gaagaagttc
     8461 atccgcgagc aaaacttggt aatgagcaag ttcaaagtaa ctttttgatg aatttagcaa
     8521 ctatcctccc ttttgggtgc aaaccaagca ttattaccga tgcgggattt aaaattccct
     8581 ggtttaaaat ggttcttaag ttgggctgga attacattgg acgtgtgcga ggagaggtac
     8641 agtttaatga ggatggtgaa ttcaaaccaa taagccattt gttccctaga gcctctacta
     8701 aagcactcta cgtggggaat ttggttttgg caaaatataa tccgttcaaa acaaattttt
     8761 atctttatac gcacaaactc aaaggtagaa aaaaaacgaa tcgaaacggg acatttgcca
     8821 aagacaaaga gtcaatagtt cac
//
LOCUS       DATNSN010000137         8425 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.137, whole genome shotgun sequence.
ACCESSION   DATNSN010000137 DATNSN010000000
VERSION     DATNSN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.137"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..690
                     /locus_tag="VAH76_002468"
     CDS             <1..690
                     /locus_tag="VAH76_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387065.1"
                     /translation="KKKRTNPEVTPFIDQKDAANPFEIYKKIGELAKIDLLKDLENRT
                     LAYLNFSLKIQTEKIAGMLNDLLNRVQNQQVIVSELYQYLLPYKGKDDLFDALIFSLK
                     RELKKLSHNFSPKTQLSSISKINLVLVPSLKLDHLPRYNPEIVNICEKLENYVFRGSN
                     KKNSFFSAKNHCGYTKEVNEIVQRILRGDLLTAEAIFHELAKINIESRKDELILLRND
                     LQSEKVCLRKL"
     gene            855..2174
                     /locus_tag="VAH76_002469"
     CDS             855..2174
                     /locus_tag="VAH76_002469"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEO1387066.1"
                     /translation="MKHQSNLSGRNVYVVDGSRTPFLKAKGVGPFCGSDLAVAAGTTL
                     LNRQPFSPDELDEVIIGSAMPGPDEANIARVVALRLGCGDKVPAFTVMRNCASGMQAL
                     DNAAIQIATGRSNLVLAGGTDAMSHAPLLFNEKMASWLANWFAAKSMGQKLALITKFK
                     PSYLAPVIALLRGLTDPIVGLNMGQTAEKVAYRFNITREQMDEFAAQSHLRIAQAYAE
                     NRMSEVAPIIDYKGCIYPQDDGIRADSTVEKLSKLKPFFDKKYGMVTPGNSSQITDGA
                     CLLLLASAEAVKKYALPVLGRIVDSQWAALDPSQMGLGPVHAATPILERHNLKPQDLD
                     CWEINEAFAAQVLGCLAAWDDEEYCKTQLGLKGAIGAPSLERLNRDGGAIALGHPIGA
                     SGARIVLHVLKSLEQRNESRGMAAICIGGGQGGAMYLERVTEVKGHE"
     gene            2167..4185
                     /locus_tag="VAH76_002470"
     CDS             2167..4185
                     /locus_tag="VAH76_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEO1387067.1"
                     /translation="MNNYKHWDLQRDKDAILWLGLNRRDAAVNSINEEVLDELNSLLH
                     EISHDKKAMGLIIYSLKEKGFIAGADVNAFSQFESSSQAVDFLQKGQTVFARLQALSI
                     PSVAMIDGFCMGGGYELALACTYRIATDDKDTRIGLPEVMLGIHPGWGGTVRLPQLIG
                     GFKALSQVILTGSAIPAAKAKSLGMIDEVVPVRQLRRAAVYFIKNKPPRHRASFIESL
                     TNSSLLRKAIAYLLRYNVAKRVRKEHYPAPYAVIDLWEKEGGVGERAYLKEVDSIEKL
                     VSQNDTAKNLIRAFLLRERLKGFAKDSHFKAAHVHVIGAGVMGGDIAAWCALRGLQVT
                     LQDKSYEQIAPAIGRAHKLYSKKLRKPRLIQAAMDRLIADPEGHGIKRADVIIEAVFE
                     NLAVKQEIMKTVEKNAKKDAIIATNTSSIPLDEISSVMSKPERLVGIHFFNPVAKMDL
                     VEIVSSSSTAKKIQQSSCAFVNQIGKLPLPVKSSPGFLINRVLMPYLMECVQLLDEGY
                     SAETIDEAALQFGMFMGPVELADTVGMDVCLAVAENLTSHFGGTIPQRLRDLVKEGKL
                     GRKTGEGFYKYKNGKVIKKKAPSHVDKTIADRLILRMVNESAACIREKVVEDSDLLDA
                     GMIFATGFAPFRGGPMNYANDFGKDKLETLFKTFESKYGERFKVDTSL"
     gene            4245..4979
                     /locus_tag="VAH76_002471"
     CDS             4245..4979
                     /locus_tag="VAH76_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEO1387068.1"
                     /translation="MEQKSKSIIRVWYLIFLLSQYSFANVYVLPEAGNTVGEIQVAFS
                     EYGETIDEVGRRFNVGYYEMVRANPGVNARYPLAANTKLIIPSRFILPNVPRKGIVIN
                     LAEYRLYYFPPDDNVVMTYPVGIGKKGWETPLGLSRITSKEINPTWRPTVKLQKAAES
                     IGAPIPSEFPPGPYNPLGQHVLRLGWPAILIHGSNRVDGIGAKVSAGCIRMLPEDIEH
                     LYSLVSVGTPVRIINAPLQRQADFKQ"
     gene            complement(5016..5906)
                     /locus_tag="VAH76_002472"
     CDS             complement(5016..5906)
                     /locus_tag="VAH76_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO1387069.1"
                     /translation="MQVIPFRYFILLAGTIVSLSLVVLVINHFIYQFPGNNYFPPNTP
                     VMACIILLNYIGLILFFGKKNRVTCSGLELINFFIVMSIIALATNAVQLTPYPPIDKQ
                     IIKLEAQLGINMISILEWTNKYQHFKYLLAIIYDTLPYQMSILPLCVIVSGRFYILKD
                     YYFLLLCTTLMGFSYYYFFPTTAPASELQSSLFAPEQIATGFKFHQIHNHIHPTTIEG
                     GLIALPSFHTIWAILCVYLIKDWFIPCLLLSIINVLLILSCVLLGWHYPIDILAAIIL
                     VGLSYYLLTVIKVEARYLDL"
     gene            complement(5917..6114)
                     /locus_tag="VAH76_002473"
     CDS             complement(5917..6114)
                     /locus_tag="VAH76_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEO1387070.1"
                     /translation="MDIINLQFEEPLIIRISNTVVKILAFKTQENGNIKFGVEAPRSI
                     NIHREEVFHAIKQKETLSTAD"
     gene            6423..6761
                     /gene="rpsF"
                     /locus_tag="VAH76_002474"
     CDS             6423..6761
                     /gene="rpsF"
                     /locus_tag="VAH76_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO1387071.1"
                     /translation="MRHYEIMFLVHPDQSEQVPGMVERYEGIITKHNGKIHRKEDLGR
                     RQLAYSINKVHKAHYILMNVECNLDALNEIKNAFKFNDAILRHLITVQKQAITTESVL
                     MKKEKETKVA"
     gene            6775..7002
                     /locus_tag="VAH76_002475"
     CDS             6775..7002
                     /locus_tag="VAH76_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO1387072.1"
                     /translation="MSAYFRRKKMCRFSAEGGNEIDYKDINLLKNYITETGKIVPSRI
                     TGTQTRFQRQLAKAIKHARFIGLLPYCDSHR"
     gene            7004..7915
                     /locus_tag="VAH76_002476"
     CDS             7004..7915
                     /locus_tag="VAH76_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387073.1"
                     /translation="MNLAGNFILRQSRATLESKQYAIVCAVVLSIVPFASWLSVALMS
                     LITLRKGERPGFEVMLPAMIVHSVPLMMFLPLEGALINTLVAYLPCYIAALTLRKTAS
                     WSNVFGAFLIQAFLVFLSIEILIPDFVSDQFNHFKVLLSQFQDYKQLVEGSTDGLNNL
                     SLAQLFFGIQILSVIASALISLMFARTIQAKLFMPGGFKQEILEFRSGRFSFLMLIIV
                     SVASYFEVPVAISTLPLLLAYFFLSGFSLAYYILARKWQVRVVVLLFLLILLKPFFVL
                     FAYIVFGSLDSLFNFRLYLPARVREST"
     gene            7928..8377
                     /gene="rplI"
                     /locus_tag="VAH76_002477"
     CDS             7928..8377
                     /gene="rplI"
                     /locus_tag="VAH76_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO1387074.1"
                     /translation="MEVILLEKVRNLGNLGDKVHVKSGYGRNYLIPQNKAVFATEQNI
                     ELFEKRRAELEKKAQQALANAEQRAAKLNDTTIVISAMASDEGKLYGSVGVNEIKDAL
                     IEKQIEISKREIVMPEGPLHSIGNYVVEVHVHSDVVANLQVEIIPAK"
BASE COUNT         2728 a         1375 c         1771 g         2551 t
ORIGIN      
        1 aagaaaaaaa gaacaaaccc ggaggtcact ccatttattg atcaaaaaga tgcagcaaat
       61 cctttcgaaa tatacaaaaa gataggtgaa ttagccaaga tagatctact gaaggatttg
      121 gaaaaccgta cacttgccta tctgaatttc tcattaaaaa tccagacaga aaaaatagcc
      181 ggcatgctaa atgatttatt aaatcgtgta caaaatcagc aagttatagt ttctgaattg
      241 taccaatatc ttctacctta taaaggcaag gatgatttgt tcgatgcctt gatattttca
      301 cttaaaagag aattaaaaaa attaagtcat aatttctctc ctaaaacaca actttccagc
      361 atatcaaaga taaatctcgt gcttgttcca agtttaaaac ttgaccatct gcccagatac
      421 aatccagaaa tagtgaatat ttgtgaaaaa ttggaaaact atgtctttag aggatccaat
      481 aagaaaaatt catttttttc agcaaaaaat cattgtggct ataccaaaga ggtaaatgaa
      541 atagtgcaaa gaatattgag gggagatctt ttaacagcag aagcaatttt tcatgaatta
      601 gctaaaatta atatagagag tcgtaaagat gaattgattt tgcttagaaa tgatttgcaa
      661 agtgaaaagg tatgcttaag aaagctttaa tagaataagg cttgaagaat aaacacatcc
      721 aggaatgatt tacgtaattt aatgtcatca aaaaaatcat cttaagattt ccaaaatatc
      781 aagaatactg ctaatctttg aatctgttga ttttagtata gattcttatg cagaggccct
      841 aacaaggagg actaatgaaa caccagtcga atttaagtgg tcgtaacgtt tatgttgtcg
      901 atggaagtcg aacgcctttt cttaaagcaa aaggagtagg tccattctgt ggttcagatt
      961 tggcagttgc tgcaggaaca actttgctaa atcgccaacc attttctccg gacgagcttg
     1021 atgaggtcat tattggtagc gctatgccag gaccagacga ggccaatatc gctcgcgtgg
     1081 ttgctctgcg tttgggatgt ggtgataagg tgcctgcatt tactgtaatg agaaattgtg
     1141 cgtcaggcat gcaggcattg gataatgccg caatacaaat tgctacaggg cgcagtaatt
     1201 tggtcctcgc tggtggaaca gatgccatga gccatgcacc gttgttattt aatgaaaaaa
     1261 tggcttcctg gctggcaaac tggtttgcag ctaaaagtat ggggcaaaag ctggctttaa
     1321 ttacaaagtt taaaccttct tatctggcac cggtaatcgc tttgctcaga ggcctgactg
     1381 atccgatagt aggattgaat atgggacaaa cagctgagaa agtagcttat cgatttaaca
     1441 tcacaagaga gcaaatggat gaattcgcag ctcaaagcca tttaagaata gctcaagctt
     1501 atgctgaaaa tagaatgtct gaagttgccc caattataga ttacaaaggt tgtatatatc
     1561 ctcaagatga tgggattagg gcagattcta cggtggaaaa attgtcaaaa ctaaaaccat
     1621 tttttgacaa aaaatatggc atggtcaccc caggtaacag ttcacagata actgatggag
     1681 cctgtttgtt gctattagcc agcgctgagg ctgtaaaaaa atatgcttta ccggtattag
     1741 gccgaatagt tgattcacaa tgggctgctc tagatccctc tcaaatgggt ttgggtcctg
     1801 ttcatgcggc aacccctata ttagaaagac ataacctaaa gccacaggat ttggattgtt
     1861 gggaaatcaa tgaagccttt gccgctcaag tcttagggtg tttggctgca tgggatgatg
     1921 aagaatactg caaaacacag cttggcctta agggggcaat tggagcgccg tcgctggagc
     1981 ggctcaatcg ggatggagga gcaattgcgt taggtcatcc tattggtgcc agtggggctc
     2041 gtattgtgtt gcacgttctt aaatctttag agcaaagaaa tgagtcgcga ggtatggcag
     2101 caatttgtat cggaggtggt cagggaggag ctatgtatct tgaacgagtt actgaggtga
     2161 aaggacatga ataattataa acattgggat ttacaacgag acaaagatgc cattctgtgg
     2221 cttggattaa atagaagaga tgctgctgtt aatagtatta atgaagaagt cttggatgag
     2281 ttaaacagct tgctgcatga aatatcgcat gataaaaaag caatgggatt aattatttat
     2341 tcgctcaagg aaaaagggtt tattgcgggt gctgatgtta acgctttttc acaatttgag
     2401 agctcttcac aagcggttga ttttttgcag aaaggacaaa ctgtatttgc ccggttgcaa
     2461 gctttatcaa ttccaagtgt cgcaatgatc gatggttttt gcatgggcgg tggttatgag
     2521 ttagctttag cttgcactta ccgaatcgca acggatgaca aagacacacg tattggtttg
     2581 cctgaagtaa tgctgggtat tcatccaggg tggggcggta ctgtgcgttt gcctcagtta
     2641 ataggtggtt ttaaagcttt atcacaagtc atattaaccg gtagtgcaat acctgctgca
     2701 aaagctaaaa gcctcggcat gattgatgag gtagtacctg ttcgtcagct aagaagggct
     2761 gctgtttatt tcataaagaa taaaccacct cgacacaggg catcatttat tgaaagttta
     2821 acgaatagct ctctgcttag aaaagctatc gcttatttac tgcgttataa cgtagccaag
     2881 cgagttcgaa aagaacatta tcctgcacca tatgccgtga ttgacctgtg ggaaaaagaa
     2941 gggggagtgg gagagagggc ttatcttaaa gaagtggact ctatagaaaa acttgtatcc
     3001 caaaatgata ccgccaagaa tcttatcaga gcatttttat tgcgtgagcg attaaaaggg
     3061 tttgccaaag acagccactt caaagcggct catgttcatg tgattggagc tggagttatg
     3121 ggaggggata tcgcggcctg gtgtgctttg cgaggtcttc aggtcacatt acaggataaa
     3181 tcttatgaac aaattgcccc tgcaataggg agagcacata aactttatag taaaaaactg
     3241 cgtaagccgc gattgatcca ggcagcaatg gatagattaa tcgcagaccc cgaagggcat
     3301 gggataaaac gagcagatgt cattattgag gctgttttcg aaaatttagc tgttaaacag
     3361 gaaataatga aaactgtgga aaaaaatgcc aaaaaagatg cgatcatcgc gacaaacacc
     3421 tctagtatcc cacttgatga aatcagtagt gtaatgagta aaccagaacg cttggtaggc
     3481 attcatttct ttaatcctgt agctaaaatg gatttggtag aaatagtcag cagctcatct
     3541 actgcaaaaa aaattcagca gagctcttgc gcgttcgtca atcaaattgg caaattgcct
     3601 ttgcctgtca aatcaagccc tgggttttta ataaacagag tgctcatgcc ttatctaatg
     3661 gagtgcgtac agttattgga tgaaggatat agtgcggaga caatagatga ggccgcatta
     3721 cagtttggta tgtttatggg ccctgttgaa ctggctgata ccgtaggaat ggatgtttgt
     3781 cttgctgtgg ccgaaaatct gaccagtcac tttggtggta ctattccaca aagattacgt
     3841 gacttagtaa aagaaggtaa gctaggcaga aaaactggag aaggttttta taaatataaa
     3901 aatggaaaag taattaagaa aaaggctccg tcacatgttg ataaaaccat tgcagacaga
     3961 ttaattttac gaatggtgaa tgaatctgca gcttgtatac gtgagaaagt ggttgaagat
     4021 tctgacttgt tggatgctgg aatgatcttt gccacaggtt ttgctccatt ccgtggaggg
     4081 cctatgaatt acgcaaatga ttttggtaaa gataaactgg aaactttatt taaaacattt
     4141 gagtctaaat acggcgaacg ttttaaggta gataccagct tataatttct ctgcaaggta
     4201 agacgttatg gttttacctt gctttttaga gagaaaagca tcatatggag caaaaatcaa
     4261 aatctataat aagagtttgg tatttaatat ttttactcag tcaatattct tttgcgaatg
     4321 tttatgtatt gcctgaagcg ggaaataccg tcggtgaaat tcaggtcgca ttttcagaat
     4381 atggagaaac tatagatgag gtcggccggc gttttaatgt cggttattat gaaatggtac
     4441 gtgctaaccc gggagtgaat gccagatatc cgctagccgc taacacaaaa ttaattattc
     4501 catctcgttt tatccttccc aacgtgccca gaaaaggtat agtgattaat ttagcagaat
     4561 atcgattata ttattttcct ccagatgaca atgttgtcat gacttatcct gttgggattg
     4621 gtaaaaaagg atgggagact cctttgggat taagcagaat cacctccaag gaaattaatc
     4681 ctacctggcg acctacagta aaattacaaa aggcagctga aagcattggt gctcctattc
     4741 ccagtgagtt tccccctggc ccttacaatc ctttagggca acatgtattg cgtttagggt
     4801 ggccagctat cttaatacat ggttctaatc gagttgatgg gattggagcc aaagtcagtg
     4861 cggggtgtat tcgtatgctt cctgaagata ttgagcacct atatagttta gtgtctgtag
     4921 ggacgccagt aagaattata aatgcacctc tacaaaggca agctgatttt aagcaataac
     4981 tattttcagg tatggatttt cattcaggtt aatgcttata aatccaaata acgggcctca
     5041 actttaatta cagttaacag ataataagat aatccaacaa gtattatagc cgccaaaatg
     5101 tcaatcgggt aatgccatcc cagcagtaca catgaaagga ttaatagaac attaatgatt
     5161 gacagcaata ggcaggggat aaaccagtct ttgattaagt agacgcacaa aattgcccag
     5221 attgtatgaa atgagggaag agctattaaa ccaccttcaa tggttgtggg gtgtatatga
     5281 ttatgaattt gatgaaactt aaatcctgta gctatttgtt caggggcaaa taaactgctt
     5341 tgtagttcac ttgcaggtgc ggtcgttggg aaaaagtagt aataagaaaa ccccattaat
     5401 gttgtacaga gtaataggaa ataataatct ttaaggatat aaaatcgccc tgatacaatt
     5461 acgcaaaggg gtaagatact catttgatat ggtagggtat cgtagataat cgccaataag
     5521 tatttaaaat gttgatattt attcgtccac tccagaatag atatcatatt aatacccagt
     5581 tgcgcctcca gtttaataat ttgtttgtct ataggaggat agggagttaa ttgaacagcg
     5641 tttgtcgcta aagcaatgat tgacatcaca ataaaaaaat tgatcaactc taatccagag
     5701 caagtcactc tatttttttt accgaaaaat aaaatcaatc caatataatt taacaagata
     5761 atgcaagcca tgactggagt attaggtgga aaataattat tcccggggaa ttgatagata
     5821 aaatgattga taactaaaac aactagcgat agagatacta tagtcccagc caataaaata
     5881 aagtatctga aaggtataac ttgcatttat ttgttgttag tcagctgtgc taagagtttc
     5941 tttttgctta attgcatgga aaacctcttc tctgtgaata ttgattgacc tgggtgcctc
     6001 aactccaaat tttatatttc cattttcctg tgttttaaaa gccaaaattt taaccacagt
     6061 attcgagata cgaatgataa gtggttcttc aaattgaaga tttattatat ccatttaaaa
     6121 aaaacctctt ttttcataaa ttattcctaa ggccagtatt cactgctgaa cggtacctta
     6181 ctttggtgac ggttgtcact ataaatttct gtaaaattta taaaaaaatt tatttaaagc
     6241 aactatgtga ttttgttgcc tataaaatag gcaaagttaa gatgcgcagc attaataaat
     6301 atcttatttt aaatcgcaat gagattgtaa ggattaaata aaaaagttat aattggcagc
     6361 tttttaaaac ccttgcctta ccagtactca atgctggaag gctttatcca taaggagaat
     6421 tcatgagaca ttatgaaatt atgtttctag tacatccaga tcaaagcgaa caagtacctg
     6481 gtatggttga gcgctatgaa gggattatta ccaaacataa cggcaaaata catcgtaaag
     6541 aagatttagg tcgacgccag ctggcatatt caataaataa ggttcataaa gcacattata
     6601 ttttaatgaa cgtagaatgt aatcttgacg cacttaatga aatcaagaat gcattcaagt
     6661 ttaatgatgc tattttaaga cacttaatca ctgttcaaaa acaagcgatt actacagaat
     6721 ctgtcttaat gaagaaagaa aaagaaacta aagtcgctta agaggagaac atctatgtca
     6781 gcttattttc gtagaaaaaa aatgtgccgc tttagtgctg aaggtggtaa tgaaatagat
     6841 tataaagaca ttaatctatt aaaaaattat attactgaaa ctggtaaaat tgtaccaagc
     6901 cgtattactg gtactcaaac tcgtttccaa agacaattag ccaaggcaat taaacatgcc
     6961 agatttattg gtttattgcc ttattgcgac agccatagat aaaatgaatc ttgcaggtaa
     7021 ttttatactc agacaaagta gagcaactct tgaaagcaaa caatatgcta ttgtttgtgc
     7081 ggttgttctt tcaatagttc cattcgcctc ttggctatcg gtggctttaa tgagtttaat
     7141 tactttgagg aaaggtgaaa gacctggatt tgaagtcatg ttacctgcga tgattgttca
     7201 ttctgtgcct ttaatgatgt ttttacctct ggaaggggca ttgatcaata cattggttgc
     7261 ttatttgcct tgctatatcg ctgctttgac tttacgcaaa acagccagct ggtcaaatgt
     7321 atttggagct tttctaattc aagcattcct tgtatttctg tccattgaaa tattgatacc
     7381 tgacttcgta agtgatcaat ttaatcattt taaagtgcta ttgtctcaat ttcaggatta
     7441 caaacagttg gtagaaggca gtacagatgg attaaataat ttaagtttgg ctcagttatt
     7501 ttttggtatc cagatattaa gtgtgattgc ttcagcctta atctcactaa tgtttgcccg
     7561 aactatacaa gctaagcttt tcatgcccgg tggttttaaa caggaaatat tagaatttcg
     7621 tagtggcaga ttttcatttt tgatgttaat tattgtgtct gttgcctctt attttgaggt
     7681 accagtggct attagcactt taccactact tcttgcgtat ttctttttat cgggtttttc
     7741 tttggcctat tatattttag ccagaaagtg gcaagttaga gtggtggttc ttttatttct
     7801 attgatcttg ttaaaaccat tctttgtctt atttgcctat attgtatttg gctcgctaga
     7861 tagtttattt aattttcgat tatatctacc tgcaagggtg agagaatcaa cgtaaggggt
     7921 tattaagatg gaagtaatct tattagaaaa agtacgtaat ctgggaaatc taggtgataa
     7981 agtacacgtt aaatcaggtt atggacgtaa ttatttgata ccacaaaata aggccgtctt
     8041 tgcaacagaa caaaatattg aattatttga aaaacgtcgt gctgaactag agaaaaaagc
     8101 gcaacaggct ttggctaatg cggaacaacg tgctgctaaa ttgaacgata ccacgatagt
     8161 tattagtgct atggctagtg acgaaggtaa attgtatggt tccgttggag ttaatgaaat
     8221 taaagacgca ttgatagaaa aacaaattga aatcagcaag cgtgaaatag ttatgcctga
     8281 aggccctcta cattctatag gtaattatgt tgttgaagta catgtacata gtgatgtagt
     8341 agctaacctt caagttgaaa ttatccctgc taaataattt gtttgatacc gacctaaaaa
     8401 tattcaaatc aacaatattt atata
//
LOCUS       DATNSN010000138         8413 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.138, whole genome shotgun sequence.
ACCESSION   DATNSN010000138 DATNSN010000000
VERSION     DATNSN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.138"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..350)
                     /locus_tag="VAH76_002478"
     CDS             complement(<1..350)
                     /locus_tag="VAH76_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387075.1"
                     /translation="MSILFLARKLASDYGFIEQIQQSHNRNQINYRFYSPRTDLHGSN
                     TNRLYGNPGKGVISQNTIFIYWYRDGKNSYFIECSNRLPKKELEVLHQLLMDNQHSLE
                     EILEASNNRFLSFQT"
     gene            540..1187
                     /locus_tag="VAH76_002479"
     CDS             540..1187
                     /locus_tag="VAH76_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEO1387076.1"
                     /translation="MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFL
                     DAPRRPVALNGGMVMPAWYDIYGLGFVDEEDKSGIEQSELLIRKVVDAQYNCGFKPHQ
                     IFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
                     PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ"
     gene            1184..1618
                     /locus_tag="VAH76_002480"
     CDS             1184..1618
                     /locus_tag="VAH76_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEO1387077.1"
                     /translation="MTIVKRSRTVPYSCEQMYGLVNDVEHYSEFLPYCAESKILHRDN
                     DEVQATLVIAAAGMSKSFTTRNRLQTNKMIEIRLVDGPFSHLEGFWRFDQEEKGCRIS
                     FDLEFEFAGKIFSMLLGPIFDQITDKMVDAFCERAEVIYGKG"
     gene            1605..1877
                     /locus_tag="VAH76_002481"
     CDS             1605..1877
                     /locus_tag="VAH76_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEO1387078.1"
                     /translation="MVKVELVYIADSKNCLHYKMDLKQGATVEEALIESNIYSTCPET
                     RELPVGVYGKRVSVDQVLKEGDRIEIYRPLILDPKEKRRKLAQFKK"
     gene            complement(1965..2309)
                     /locus_tag="VAH76_002482"
     CDS             complement(1965..2309)
                     /locus_tag="VAH76_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEO1387079.1"
                     /translation="MAKFFMVGKKMRIMTFFICFILTLTLTQCASYDFSRRITQQGNL
                     LPQSKIARLKTGMSKNDVAILMGTSLMSPTFNNDRWDYAYTWRRGKGNLEIRNVVLYF
                     SKGSLVKIEHKP"
     gene            2507..2917
                     /gene="fur"
                     /locus_tag="VAH76_002483"
     CDS             2507..2917
                     /gene="fur"
                     /locus_tag="VAH76_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEO1387080.1"
                     /translation="MEESQQLKDAGLKITLPRIKVLQILEQSRNHHLSAEAVYKALLE
                     SGEDVGLATVYRVLTQFEAAGLVSRHNFEGGHSVFELSQGEHHDHLVCVKCGRVEEFV
                     DEIIEQRQKAIAERAHFKMTDHALNIYGICPQCQ"
     gene            3055..3198
                     /locus_tag="VAH76_002484"
     CDS             3055..3198
                     /locus_tag="VAH76_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEO1387081.1"
                     /translation="MEFDYIIVSDGSPGWAFASRLSANPANKLLLLEAGDSVFLEMPI
                     RLQ"
     gene            3357..4427
                     /locus_tag="VAH76_002485"
     CDS             3357..4427
                     /locus_tag="VAH76_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1387082.1"
                     /translation="MHQKKRSSLELEALLSLFEGSLRTIPFNILAVMVLSLGFIYTNQ
                     IPIKLVIICFFLILILSVVRWVTSRIIISKEFYITKGKQALIGFLLLNFFMGLAWSLS
                     YFIFSPYLNDTSEFIFVFILGGLSTGAIASLSIYLPAYYAYVLPMNFPIIAYNLYLFQ
                     FDKAIFAVMYSLFLIIVLLTARLNSKLLSKVRVLSITDSLTGLFNRRHFDVVFRNELS
                     RAKRNKYPISLVFIDIDNFKYINDTFGHSVGDSFLIHVSNILKQTLRRSSDSLFRIGG
                     DEYAAILINMPPSEAITVCALLQEQFNKKNQYKNVSLSMGVISIDSIRVIDQQSAITA
                     ADKTLYQAKKAGKNQIKSKSLG"
     gene            complement(4745..5131)
                     /locus_tag="VAH76_002486"
     CDS             complement(4745..5131)
                     /locus_tag="VAH76_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387083.1"
                     /translation="MKKWTVLAASSLLMVNAYANESFCGYKDFFHLGDKTHPGIYVVS
                     GYNDSDVVLQIVGPRSFVIRDGFDCRAGYAHVTVAYDNANWCVLDINDGPFMNHPVVS
                     ASCNGLRYINTTYDGFGSYSYSINIE"
     gene            5387..5986
                     /locus_tag="VAH76_002487"
     CDS             5387..5986
                     /locus_tag="VAH76_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0375 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387084.1"
                     /translation="MYSSLFLKKDLGCLPEKVIFLDFDGTVALPARKGNGEYDIQPDG
                     SIGATHLNKEKFRRLVQFAIQTDIPLYFLSGRPDLQSCVDLMTDFIASVDGFHSGIGG
                     FKKDSLFFISKMIIEEGKLVRKEITTKAQVIQSVHHEKYSYLPKESILLVDDIQEYLD
                     PAQELGYNTLLANPESLEHFDEVEKFIGVTLQKTIAFNT"
     gene            complement(6026..>8413)
                     /gene="sdhA"
                     /locus_tag="VAH76_002488"
     CDS             complement(6026..>8413)
                     /gene="sdhA"
                     /locus_tag="VAH76_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HEO1387085.1"
                     /translation="GIPPELRASAISFDKFLVHSFSNESSTYDAPAVDLFEKLDEHFQ
                     IYFTEIDLQWGKKSNQYLKWAQEKFASENNSTELEHDEKLESRAHHLIKHTNFSKCIH
                     EFRTELNQVISFFNNAMRAQLKQQPNGIPYPELQDQNKELAQSEQVVAIKRIFNSLYH
                     VEKIVVELEKLDTRSYESIYVYHLIQAYDHINEIKKLALKLAVDPHFKLIASELLEKA
                     QTLIATIQEHSDAYQVSPLEVPYNQDIKYNALWYTLNAFYISPKHIRSLRNTNYLTTE
                     ELNDLHLKAKKATVTVENIIKSSNSYFKLFLQTPAMYSLYREMTNKLNEFISTSHDAV
                     MNNLDQFRSKIFTPMLMEADAWEDKLGLVPGTISGPLKTITDEYYKGLLHPLALHSKA
                     HIRMICDKTPIDQRIKTTNKKLENATQHLDKIEKNYKHIIKLYEIIKTKGGKTTDDEL
                     IETYKKALYKLVKLQKRLPQNTDHDPKDYELDTLLNSELKEYEPKLTLIKPLIISSYH
                     HYEGLKATYLMKANTAREKLSYLKELRSTQEQEDLLYIEEYTTESFNKQLDAFCNRHI
                     GLQYTDKEYRTKLREYLLTFKESIIQRSKSAEDINLTIKNLLKEKIKNFERDNFEKYY
                     HLDAVRVALAQFKNYFSLSTAAIEQNNSTFESEETLAKKTELINKLVDISEDGTLTPK
                     ERVDQISFHVKNPNFERIIMDYKQESYFSFTYLKQCILSLLEALCLYTPERRKLFNNL
                     ESSVKTQPKINELTKRFGLFAGNESAISTESRAITPVSSVENNGTIELNQLAPST"
BASE COUNT         2498 a         1354 c         1663 g         2898 t
ORIGIN      
        1 gtctgaaaag agagaaatct attattggat gcctcgagta tctcttccaa agagtgttga
       61 ttatccatga gcaattgatg caacacttca agttcctttt taggtagtct atttgaacat
      121 tcaataaaat aactgttttt tccatctctg taccagtata tgaaaatcgt attttgtgaa
      181 atgacacctt tgccaggatt tccatataac ctgtttgtat tggaaccatg cagatcagtt
      241 cgtggggaat aaaagcgata atttatttgg ttacgattat ggctttgctg gatttgctca
      301 ataaatccgt agtcagaagc cagttttctt gctaaaaaca aaatactcat aaaacagcat
      361 aacatacaaa acaaacataa tgataaagat ttatacagta aagtcaatag ttattagaat
      421 tcccagtgtc aatatgtcta taaattgttc aatatatttt taaatgttaa acttgcgctg
      481 ctctattgca ttggcgataa tttacgtttt tgcgtggaga atttgttttg agcgtttata
      541 tgaaggaacc tttagagaaa gcacaagctt gtgttatatg gatgcatggg ttgggagctg
      601 atgcctctga tatgatgggt ttagctgatc agttaacagt agaagatacc gctttaaggc
      661 atgtatttct tgatgctcct cgaagaccag tggctctgaa tggaggaatg gttatgcctg
      721 cgtggtatga catttatgga ttaggctttg tcgatgaaga agataagtct ggcattgagc
      781 aatctgaatt gttaatacgc aaggtggttg acgctcaata taattgtggc tttaaacctc
      841 atcaaatctt tttagctggc ttttctcaag ggggagctat ggcattgcac acggctcttc
      901 atatgactga gcgattatgc ggcgttattg cattgtccgc ttatttgccc ttggctaagc
      961 ataataagcc gcaacttgat aagaatactc ctatctttat gggggcagga caatttgatc
     1021 ctttagttct gccaaaatgg actctacaga gtaaggattg gctcttggct aatgggtata
     1081 atgaagtctc ttttcatcaa tacccaatgg aacattccat atgttttgaa gaaattaaag
     1141 atcttagcct ttggcttaat aagcaggttc aaggagtttt acaatgacaa tagtaaaaag
     1201 gtcaagaaca gtaccttatt cttgtgagca aatgtatgga ctggttaatg atgttgaaca
     1261 ttattccgag tttctacctt attgtgcgga aagcaaaata cttcatcgtg acaatgatga
     1321 agtccaggcg actttggtga tagcagctgc tggaatgagt aagtcattca ctactcgtaa
     1381 tcgattgcaa actaataaaa tgattgaaat tcgtctggtt gatggtcctt ttagtcattt
     1441 agagggattt tggcgttttg atcaggaaga aaaagggtgc agaatttctt ttgatttgga
     1501 gtttgagttt gccgggaaaa tattttccat gcttcttggt cctatatttg atcagataac
     1561 tgataaaatg gttgacgcat tttgcgaaag agccgaggtc atttatggta aaggttgaat
     1621 tagtctatat agcggattct aaaaattgcc tgcattacaa aatggattta aaacagggag
     1681 ctacagttga agaggcactc attgaatcaa atatttattc aacttgtcct gaaacgaggg
     1741 aattacctgt aggagtatac gggaagcggg tttctgtgga ccaggtattg aaagaaggag
     1801 atcgaattga gatttatagg ccattgatac tcgaccccaa agaaaaacgc cgtaaattag
     1861 cacagtttaa aaaatagagg catattaggc attttaatct acactgaact cttacaaaca
     1921 actttcccct tgtctaatca cttagacaag ggggagatat taaattacgg cttatgttct
     1981 atttttacta aagaaccttt ggaaaaatat aacacaacat tacgaatttc gagattacct
     2041 tttcctcgcc gccaggtata agcataatcc cacctatcat tgttgaatgt tggactcatt
     2101 aagcttgtac ccatcaagat tgcaacgtca ttcttactca tgcctgtttt taaccgggca
     2161 attttggatt gaggcaacaa attaccttgc tgagtgatgc gccttgagaa gtcatatgat
     2221 gcacattggg ttaaggttaa tgttaaaatg aaacaaataa aaaaagtcat tattcgcatt
     2281 tttttgccta ccatgaaaaa tttcgccata ataacatgtc atttattgaa aggcacctgt
     2341 catcatgtgg aatttggtat ttgccattat tcggccagac taagttttaa ggccgctaat
     2401 ggtttttaga gcatgaaaat tactttaggc cttgatgcta attattaatc caattttgct
     2461 ggaataatga tcaaagctcg aaattcgtta cagttaagga gtacaagtgg aagagagtca
     2521 acagttaaaa gacgctggat taaaaatcac attacctcgt atcaaggtat tgcaaatatt
     2581 ggagcaatca agaaaccatc atttgagtgc agaagcagtc tataaagcct tattggaatc
     2641 cggtgaggat gttggtttag ctactgtgta tagagttctt actcaatttg aagcagccgg
     2701 attagtatcg agacataatt ttgaaggagg acactcggtg tttgagttgt ctcagggcga
     2761 acatcatgac catcttgttt gtgttaagtg tgggcgggta gaggaatttg tagatgaaat
     2821 aatagaacaa agacaaaaag caattgccga acgtgctcat tttaaaatga cagatcatgc
     2881 gctaaacata tatggtatat gtccgcagtg tcaatgactt tgatctttta actggattgg
     2941 cttgtcacct cacggaatat tctaagcgta tttactatat tcctttgcag tgctttatca
     3001 ttacaataat cagagtgttt tggtaagacg ctattttctt taaggattca tcacatggag
     3061 tttgattata ttattgttag tgatggtagc ccaggatggg cttttgcttc caggttaagt
     3121 gccaatcccg ccaataaatt attgttgctg gaagcgggag atagtgtatt tctggaaatg
     3181 ccaatccgcc tgcaatgaga atcgcagaac aattatcaca ctatattcca acaactcttc
     3241 gtgagatttc catattctaa atgggatttt aaattatttg ctgggcggta aagagcaact
     3301 ctgtaaacta gtctatactt gaaattataa tgaaggtgct tgggttttga ataattatgc
     3361 atcaaaagaa gcggtcttca ttggaacttg aagccttact gtcgttattt gagggatcct
     3421 taagaactat cccattcaat atcctggcgg tgatggtgct ttctttgggt tttatctata
     3481 cgaaccagat tccaattaaa ctggttatta tctgtttttt tctcatttta attttaagtg
     3541 tggttcgttg ggtaactagc cgaattataa tcagtaaaga gttttacatc acaaaaggaa
     3601 agcaggcttt aattggtttt ttactgctga atttttttat gggactggct tggagtttga
     3661 gctactttat tttttctcct tatttaaatg atacaagtga gtttattttt gtattcattc
     3721 tggggggatt atcgacaggc gctatcgctt ctttatcaat ttatcttccc gcctattatg
     3781 cttacgtatt gccaatgaat ttcccaatta ttgcctacaa tttgtattta tttcagtttg
     3841 ataaagcaat ttttgctgtc atgtattcgc tttttttgat catagtattg ttgacagcaa
     3901 gattaaattc aaaattactt tccaaagtaa gggtattatc tataaccgat tcattgactg
     3961 gattatttaa ccgtcgtcat tttgatgtgg tgttcagaaa tgaactaagc agggcgaaac
     4021 gtaataaata tcctatcagc ctggttttca ttgatataga taatttcaag tatattaacg
     4081 atacctttgg tcattctgta ggtgacagtt ttttaattca tgtatcaaat atcctgaagc
     4141 aaacattacg acgttccagc gatagcttgt ttcggattgg tggagatgaa tatgctgcca
     4201 ttctgattaa tatgccacca agtgaagcaa taacagtttg tgctttgttg caagaacaat
     4261 ttaataaaaa aaatcaatat aaaaatgtgt cgttaagtat gggggtcata agcattgatt
     4321 ctataagagt gattgatcaa caaagcgcca ttactgcagc ggataaaact ctttatcaag
     4381 caaaaaaagc agggaaaaat caaattaaat ctaagtcgtt aggctagcaa gaattgagag
     4441 tcaattaaaa ttgttggatt tgattgcagt aagtatgaga taagagaata caattttatt
     4501 tctcttattc ttatatcaga gacggattct ggattaatta cttttaacgt gagttataga
     4561 caagatagct cgttttcttg aatcgtttgg gctgaggagg catttatgcc gtctcgaagc
     4621 cttgtatgaa atttaatgtt tcttgccagg gctttgagaa gatgctcatg tatctcctca
     4681 gcccagacag accctggata tcgattgctt cttatataat cgctatccat aactcacgtt
     4741 acttttattc aatgttgata gaataggagt aacttccaaa gccatcatag gtggtattga
     4801 tgtaacgaag accattgcaa gaagcactga caacaggatg attcataaat ggaccgtcat
     4861 taatatcaag tacgcaccaa ttggcattat cataagctac agtgacatgc gcatagcctg
     4921 ctcgacaatc aaaaccatct ctgattacaa agctacgtgg cccaacgatt tgtaatacga
     4981 catctgagtc attataacca ctgaccacat aaattccagg gtgagttttg tcacctaaat
     5041 gaaaaaaatc cttgtaccca cagaatgatt cattggcgta ggcgttcacc attaaaaggc
     5101 tacttgctgc cagtactgtc cattttttca tacgaaaata ccttcttatt gttattcagg
     5161 gaatcgaatt atagggaatc gatggctatt aaacaatcat cttgttcttt tgtttttgaa
     5221 aaacctgttc tgcaagtgat ttctggaaag ggtaggaaat atttcctgtt aaagagatga
     5281 tcagttacct tgtttcgatt gatctaatcg gatacgctga ccagttgata ttaagaatct
     5341 ccacaatatt ttgaagggat agaaacatta agtgtcagga ggaaaaatgt attcatcatt
     5401 atttctaaag aaggacttgg ggtgtttacc tgaaaaagtt atttttcttg attttgacgg
     5461 caccgttgct cttcctgcaa ggaagggaaa tggagaatat gatattcagc ctgatgggtc
     5521 aattggagct acccacctaa ataaagaaaa atttagacgt ttagtacagt ttgctattca
     5581 aactgatatt cctttgtact ttttgagcgg acgtcctgat cttcaatcgt gtgttgattt
     5641 aatgactgat tttattgcaa gcgtcgatgg cttccattct ggtattggcg ggtttaaaaa
     5701 ggattctctg ttttttatca gtaaaatgat aattgaagag gggaaacttg ttcgaaagga
     5761 aataacgaca aaggctcagg ttattcaatc cgtgcatcat gaaaaataca gttatttacc
     5821 taaggaatcg attttactgg tcgatgacat tcaagaatac ctcgacccag cgcaggaact
     5881 gggttataac actttgttgg caaatccgga gagtttggag cattttgatg aagtagagaa
     5941 atttattggg gtaacattac aaaaaactat cgcattcaac acctagacaa ggttatggta
     6001 aattaaaaga ttaaatcaaa tatttttatg ttgatggcgc taattggttt aattcaattg
     6061 ttccattatt ttcaacagaa gaaactgggg taattgccct ggattcagta gagatagctg
     6121 actcattgcc agcaaataat ccaaatcgtt tggtcagttc attgatttta ggctgggttt
     6181 ttacagagct ttccaaatta ttaaatagtt ttcttctctc cggagtgtac aggcaaagcg
     6241 cttccaataa ggataagatg cattgtttta aataggtaaa gctaaagtag ctttcctgtt
     6301 tgtaatccat gatgattctt tcaaaattag gatttttaac atggaaagat atttggtcga
     6361 cacgctcttt tggcgtaagg gttccatctt cactgatatc aaccagttta ttaatgagct
     6421 ctgtcttttt cgccagtgtc tcttcgcttt caaatgtcga attattttgc tcgatggcag
     6481 cggtagaaag actaaagtag tttttaaatt gagctaaagc aactcttact gcatcaagat
     6541 gataatattt ttcaaaatta tctctttcaa aatttttaat tttttccttt agcaaatttt
     6601 ttatagttaa attaatatct tctgcggatt ttgagcgttg aataatactt tctttgaatg
     6661 tcagtaaata ttctctaagt tttgtgcgat attccttgtc agtgtattgt aaaccgatat
     6721 gacggttgca gaaagcatct aattgtttat taaatgattc tgtagtgtat tcttctatat
     6781 aaaggagatc ttcttgttcc tgagtcgatc gtaactcttt aagataactc aacttttctc
     6841 tggcagtatt tgctttcata agataagtgg cttttaggcc ttcataatga tggtagcttg
     6901 agataatgag aggcttaatg agcgttaatt taggttcata ctctttcagc tctgaattta
     6961 aaagagtgtc taattcataa tctttaggat catgatctgt attctggggt aatcgctttt
     7021 gtaattttac caatttgtac agagcttttt tataagtttc gattaattca tcatcagtgg
     7081 ttttgccgcc ttttgttttg ataatttcat atagcttgat gatatgttta taattttttt
     7141 ctattttatc caaatgttgt gttgcatttt cgagtttttt attcgttgtt tttattcttt
     7201 gatctattgg tgttttatca catatcatgc gaatatgtgc tttggaatgc agcgctaaag
     7261 gatgtaacag gcctttataa tattcatccg ttattgtttt taatggtcct gagatagtac
     7321 ctggtaccaa gcctaattta tcttcccatg cgtccgcttc catcagcatg ggtgtaaaaa
     7381 tcttggagcg gaattgatcc agattattca ttacggcgtc atgtgatgtg ctaataaatt
     7441 cattcagttt gttcgtcatt tctctataga gagaatacat ggcaggcgtt tgtaagaata
     7501 atttgaaata ggagtttgaa cttttaatga tattttctac agtaacggtt gctttttttg
     7561 cctttaagtg taagtcgttg agttcttctg tcgtgagata gttggtattt cttaaacttc
     7621 taatgtgttt tggtgaaata taaaaagcat tgagtgtata ccaaagagca ttgtatttaa
     7681 tatcctggtt ataaggtact tctaacgggg atacctgata cgcatcactg tgttcttgta
     7741 ttgttgcaat aagagtttgc gctttttcca gtaactcgct tgctatgagc ttaaaatgag
     7801 gatccacagc gagttttagc gctaatttct taatttcatt aatgtgatca taggcttgta
     7861 ttaaatgata gacataaatg ctttcgtaac ttctggtgtc cagtttttcc agttcaacaa
     7921 caatcttttc aacatgatag aggctattga atatgcgttt tatcgcaaca acctgttcac
     7981 tctgggcaag ctctttattt tgatcttgca attcagggta aggtattcca ttgggttgtt
     8041 gttttaattg cgccctcatc gcattattaa aaaacgaaat gacttgatta agctccgttc
     8101 taaactcatg tatgcattta gagaaattgg tatgtttaat taaatgatgg gctctgcttt
     8161 ccagtttttc atcatgttca agttcggttg agttattttc actggcaaat ttttcctggg
     8221 cccattttaa atattgatta ctctttttac cccattgtaa atcaatttct gtaaaataaa
     8281 tttgaaaatg ttcatccagt ttttcaaaca ggtcaactgc cggggcatca taggtggatg
     8341 actcattaga aaaggaatga accaaaaatt tgtcaaaact tattgcggaa gctctgagtt
     8401 caggaggtat ccc
//
LOCUS       DATNSN010000139         8370 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.139, whole genome shotgun sequence.
ACCESSION   DATNSN010000139 DATNSN010000000
VERSION     DATNSN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.139"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(52..420)
                     /locus_tag="VAH76_002489"
     CDS             complement(52..420)
                     /locus_tag="VAH76_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEO1387086.1"
                     /translation="MSVEIQQASSTNPNITFSESAIKHLVTYLEKNPGNKGVRLSVKK
                     TGCSGLSYVVDYVKEPSEGDMIASLSKDYLVCVDKTSYPFLKNMQVDYVKQGLNYKFI
                     FNNPNQTGQCGCGESFTVSQ"
     gene            complement(425..808)
                     /locus_tag="VAH76_002490"
     CDS             complement(425..808)
                     /locus_tag="VAH76_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEO1387087.1"
                     /translation="MMYNKIVQDYFFQPQHIGKLDLNDPFVIGVQSQPINQLNTVHFY
                     IKCDGDKLISKARFKTNGNPYVIAAMEWICCQIEGKTLESLPLIDFQILINVLQITNN
                     QYPVAVQVEDIYKEALTLMRKKFER"
     gene            complement(805..1974)
                     /locus_tag="VAH76_002491"
     CDS             complement(805..1974)
                     /locus_tag="VAH76_002491"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEO1387088.1"
                     /translation="MKLAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATH
                     EYGRVASMAVEQARSQIAETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVT
                     MSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEI
                     GVIQDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGA
                     LYVRHKPRIRLQPLSYGGGHEGGLRSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNL
                     RKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSELAISTTSACSSA
                     SIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL"
     gene            complement(1943..2716)
                     /gene="trmJ"
                     /locus_tag="VAH76_002492"
     CDS             complement(1943..2716)
                     /gene="trmJ"
                     /locus_tag="VAH76_002492"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HEO1387089.1"
                     /translation="MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPD
                     LKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELIN
                     QQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
                     LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRIN
                     LEKMEVSILRGMLSQVQKSLEWARKDNRSNKIGNKTDLF"
     gene            2832..3617
                     /locus_tag="VAH76_002493"
     CDS             2832..3617
                     /locus_tag="VAH76_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEO1387090.1"
                     /translation="MEPLLNIAVNAARQAGEIINRYVEQVDRLKITPKNSHEYFSEVD
                     IKAEQVIINTIHKAYPEHGILAEESGIQQSDSDTVWIIDPLDGTSNYLHGFPFYSVSI
                     ALKIKNRLEHGVIYDPLRHECFAASRGRGARLNDRRIRVSKQTQLNASLLGTGFHFRD
                     ATLAQRYLPTFEALIGKCAGVRRTGSAALDLAYVASGRLDGFWEFGLRPWDIAAGALL
                     VREAGGLISDVQGGEDFLNYGDVVAGTPKVFKSLLQTISPVLK"
     gene            complement(3730..4686)
                     /locus_tag="VAH76_002494"
     CDS             complement(3730..4686)
                     /locus_tag="VAH76_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEO1387091.1"
                     /translation="MNNESSSNSNLDSQALLNQLVIDYLQERKRKRRWKWIIRAIILV
                     VLVYSSYKIVSVAKTTEASKDKEHIGLIDINGEIADSSSASADNFTKGIEKAYKSKGL
                     KALIVRINSPGGSPVQAEYIYNVLQYYKSLKPNIKTYAVCVDMCASAAYYVAVGADEI
                     YASPASMVGSIGVLYNGFGFVDALQKLGISRRLQTSGVNKAFLDPFSPTTEFQKEKLQ
                     TMLDIVHKQFITRVKEGRGNRLHVDDETFSGLFWTGEQALANGLIDGYASSGQLAREV
                     IKITDIVDYTYKQNVFDRLTKNLGTAMADELPVILGMKPGLR"
     gene            4775..7351
                     /gene="clpB"
                     /locus_tag="VAH76_002495"
     CDS             4775..7351
                     /gene="clpB"
                     /locus_tag="VAH76_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEO1387092.1"
                     /translation="MRMDKLTSKFQMALADAQSLALGRDNGFIEPEHLMKALLDQQGG
                     SCRPLLSKAGVNIPLLRTLIDQALDKLPKVSGTGGDIHISNALNRLLNLTDKLSQQRK
                     DNFISSELFVLAAINEDSNLAKILKQAGGDNKAIEKAIDELRGGETVNDPNAEEQRQA
                     LEKYTLDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNKRLLALDMGALIAGAKYRGEFEERLKGVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPALVAAAMLSHRYISDRQLPDKAID
                     LIDEAASLIRMEIDSKPESMDKLERRLIQLKIEREALKKENDEASKKRLVDLQKSIDE
                     LEQNYSDLEEIWKAEKATMQGSTQIKEALEQAKLEMETARRAGDLSRMSELQYGRIPE
                     LEKRLSQVSSVDAMETKLVRNKVTEDEIAEVVSKWTGIPVSKMMEGEKEKLLKMEEAL
                     HSRLIGQNEAVDAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALASFLFD
                     TEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HTDVFNILLQVMDDGRLTDGQGRTVDFRNTVIVMTSNLGSQLIQEMSSKFNYDEIKAA
                     VMDLVSQHFRPEFINRIDESVVFHSLTKEQIAKIAAIQINYLHHRLKQQNITLEVTSE
                     ALSHLAEAGFDPVYGARPLKRTIQQKLENPLAQSLLTGKFKSGDTIIVSYKDGVMEFS
                     KQ"
     gene            complement(7443..>8370)
                     /locus_tag="VAH76_002496"
     CDS             complement(7443..>8370)
                     /locus_tag="VAH76_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387093.1"
                     /translation="LFSKPPSNVKDCRLFQAIRLALGLPEVMERNYRINDLKILDVTT
                     IVTALETFRDNMQLIVGKDEGKMPRYKSYPHFAADKNFETYLQEIIDEHKRRNPVVLN
                     QFKAINFIQSLVKQIEEEQRQIEEALTHLGKLLPKTCSDFKTISLELMEEQIKAQIES
                     NVLQEKIIDLLYTGHIQENFSTMDCGSFIEAMKNCNNSLARYRALGGYCLLLQNEGVK
                     EQLRFCIHQALGVEINPNELTDKDMLDAIRLLKTYFEANPKVELNFDFFGGKGSMNTF
                     ILQTELALAKKVQTVNKAQEDNSETRTTSLFV"
BASE COUNT         2620 a         1666 c         1578 g         2506 t
ORIGIN      
        1 ccagccgctt attgataata ttaaagaagc atcgtagatt ttctgcttat tttactgact
       61 tacagtaaag ctctctccac aaccacattg ccccgtctga ttgggattat taaaaataaa
      121 tttataattt aaaccttgtt tcacataatc aacttgcata tttttaagaa atggataact
      181 tgttttatca acgcatacca gataatcttt agataaagaa gctatcatat ctccttctga
      241 aggttctttt acataatcca ctacataaga taatcccgag catcccgtct tttttacaga
      301 caatcttact cccttgtttc ctgggttttt ctccagatag gtgaccaggt gctttattgc
      361 cgattcactg aaagtgatgt tggggttcgt actgctcgct tgttgtattt ctacactcat
      421 tgctttacct ctcaaatttc ttacgcatca aagtcaatgc ttctttatat atgtcctcta
      481 cctggactgc tacaggatac tgattatttg ttatttgtaa tacattaatt agaatctgaa
      541 aatctattaa tggaagtgat tccaaagtct taccttcaat ctggcaacat atccattcca
      601 ttgccgcaat aacataaggg ttaccattcg ttttaaatct ggctttggat atcagtttat
      661 ctccgtcaca ttttatataa aaatgcactg tattcagttg attaatcggt tgactttgta
      721 cacctattac aaaaggatca tttaaatcca gcttaccaat atgttgtggc tgaaaaaaat
      781 aatcctgtac aattttatta tacatcataa cggcgataat tcgtgcagcc gactcacttg
      841 tgtgcaaatt atatcaattg ccttctttat ttgcacctca gaagtgaagc gtcctatgga
      901 taaacgaata gttgattgag cctctgtatc agtcaaacca attgccctta agacataaga
      961 tggttgaata ctggcagaag aacaggcaga agtagttgat atcgctaact cacttaaagc
     1021 aaacagcaat gaatctccat ttaatccaac aaaactcaca ttcagatttc cagcgatacg
     1081 cctgtgctca tgtccattca gtttaatagc aggcaaatgt cgtattccgt cccatagttg
     1141 tttgcgtaaa tttagaatac gcgtctgttc aggaattcta tcagcctctg caatagcaaa
     1201 agcctcaccc atgccgacaa tttgatgggt aggtaatgtt cctgagcgca atccaccctc
     1261 atgcccgcca ccgtaactta aaggctgtaa acgaatcctt ggcttatgcc tgacatacag
     1321 agcccctaca cctttaggcc cataattttt atgagcagag aatgacatca aatcaactga
     1381 aagctggctt aaatcgattg gcaatttacc agcactttga gccgcgtcaa catgaaatat
     1441 aattccccta tttcgtaata gctcaccaat agatgcaata tcttggatga caccaatttc
     1501 attattaaca tgcatgacag aaaccagaat agtatctaac cttagagcag attccagttt
     1561 tccaagatcc aacaaaccat ctgattccgg attaaggtag gtgacttgaa aaccttcttt
     1621 ttccagttgg tgaaaactgt caagcacagc tttgtgttca gtgctcatag taaccagatg
     1681 cattccctta tttttataaa accgggcagc tcctaaaatc gccaaattat ccgcttctgt
     1741 agctcctgat gtaaacacta tttcttgggg tgaggcatta atagtttctg caatttgaga
     1801 gcgtgcctgc tcaactgcca tggaggcaac tctcccatac tcatgagttg ctgatgcagg
     1861 attaccaaaa tccccttcag ggcctaaata ttttatcatt tgctcaacaa cgcgaggatc
     1921 aacgggtgtt gttgccatat aatcaaaata aatcggtttt attgccaatt ttattgctcc
     1981 tattgtcttt ccttgcccac tccaatgatt tctgaacctg gctaagcata cctcttaaaa
     2041 tactaacttc cattttttca aggttgattc tattaaacag tcgcctcact ctttgcatta
     2101 atctccttgg attagagggc tttaaaaaat taatttcaat aaaaacttct ttcaaatgct
     2161 cataaaattg ctctatttca tcagctgtag ccggttcttc attgcgcaga gctacttgag
     2221 cacttggtga taataacttc atacgaagtt cataagcgat aatttgtact gcctgagcca
     2281 aatttaatga actgtactca ggattgcttg ggatattgat atgatagtga cattttaaca
     2341 gctcttcatt agtaaggcct gcgtgttctc taccaaatac aatggccact tgagtattat
     2401 ctgattgttg atttatcaat tctgcacagg aagctggaat taatccaggt aatgacagtc
     2461 cacggggtct ggcactcgtc cctaaaatca actgacatcc aattaaagct tcgtccaggg
     2521 tatttgtaac aactgctgca tccagaacat catcagctcc agcagccatt tcttttgctt
     2581 tgagatcagg aaatgacttt ggattaacca ggtacagtgt acttagcccc atggttttca
     2641 tagctcttgc agtagagcct atattgccag gatgggaagt tgaaaccaaa acgattcgaa
     2701 tagaacttaa cttcataaaa aattattcca aatacaatag ataactatat cataggattt
     2761 attaattgaa cttctctttg tttaaaaaaa atgtgttaga attgctgatt ttatagctaa
     2821 agagtatatc catggaacca cttttaaata ttgctgtcaa tgctgcgcga caggctggtg
     2881 aaatcattaa tcgttatgta gagcaagttg atcgtttaaa aataactccc aaaaacagcc
     2941 atgaatattt tagtgaagtg gacattaaag cggagcaagt tatcattaat acgatacata
     3001 aagcctatcc agagcatgga attcttgctg aagaaagcgg cattcagcaa agcgatagtg
     3061 atacagtctg gatcattgat ccattggatg gcacctcaaa ttatcttcat ggattccctt
     3121 tttattctgt atccatagcc ctgaaaatta aaaatcgtct ggagcatggt gttatttacg
     3181 atcccctgcg acatgagtgt tttgctgcaa gcagagggcg cggggcaagg ctaaacgata
     3241 gaagaatccg tgtgtccaag caaactcaac tcaatgcctc tctgttaggc acaggttttc
     3301 attttcgaga tgcaactctt gcccagcgat atttaccaac ctttgaagca ttgattggaa
     3361 aatgcgctgg agttagaaga acaggttctg ctgctctcga cttggcttat gttgcaagcg
     3421 gtcgtctgga tggcttctgg gagtttgggt tacgcccgtg ggatattgct gcgggagctc
     3481 tgctggttcg agaggccgga ggattaataa gtgatgtcca gggtggagaa gatttcctaa
     3541 attatggcga cgtagtagca ggaactccta aagtatttaa gtcacttttg caaaccatat
     3601 cacccgtttt aaaataagtt gtcaatttaa gctcatttaa gacgttcatt taggcttatc
     3661 gattttaaat tattttcgaa tatccgctct ctcgaagctg agaaagcgga taaatactcc
     3721 tctccactct tatcttaatc ctggcttcat tcctaaaatt actggcaact catctgccat
     3781 cgccgtgccc aaatttttag ttaggcggtc aaagacattt tgcttgtaag tatagtcaac
     3841 tatatcagta attttaatta cctctcgcgc caactgcccg ctactggcat aaccatcaat
     3901 taaaccatta gccaacgctt gctcgccagt ccaaaataac ccggaaaaag tctcatcatc
     3961 aacatgtaat ctatttcctc ttccttcttt gacacgagta ataaattgct tatgaactat
     4021 atcaagcatg gtttgtagct tttctttctg aaattcagtc gttggggaaa aagggtccag
     4081 gaaagcttta ttgactcctg aagtttgtaa tctgcgtgaa ataccaagct tctgcaatgc
     4141 atcaacaaaa ccaaagccat tatataaaac acctattgag ccgaccatgc ttgcaggact
     4201 tgcatagatt tcatctgctc caactgcaac ataataggca gcagaagcac acatatcgac
     4261 acaaacagcg taagttttaa tgtttggttt tagactttta taatattgta aaacattata
     4321 aatatattcc gcctgtacag gactgcctcc agggctattt atccgtacaa ttaatgcttt
     4381 taatccttta cttttataag ccttctcaat tcctttagtg aaattatcag cacttgctga
     4441 actggaatca gcaatttcac cgttaatatc aattagtcca atatgctctt tatccttaga
     4501 tgcttccgtg gttttggcga ctgaaacaat cttataagat gaataaacta atacgaccag
     4561 gataatagct cttataatcc atttccatcg acgctttctt ttcctttctt ggagatagtc
     4621 aattaccaat tgattaagca aagcttgtga atccaaatta gaattagaag atgactcatt
     4681 attcataaaa aactcttgaa aaaatgtaaa taatacccat tttatggcat gtaaactaga
     4741 attaataccc taaaataact agaaaggtgt tcctatgcga atggacaaac taacatcaaa
     4801 gtttcaaatg gctcttgcag atgcccaatc cctggcttta ggtagagaca atggatttat
     4861 agaaccagaa catttaatga aagccttgct tgatcagcaa ggcggaagtt gcagacctct
     4921 tctcagtaaa gcgggtgtta atataccact attaagaact ttaattgacc aagctctgga
     4981 taaactccct aaagtatcag gcaccggagg agatattcat atatctaatg cattgaatag
     5041 actacttaac ctgactgaca aattatcgca acaaagaaaa gataacttta tttcaagcga
     5101 gctttttgta ttagcagcga taaatgagga tagtaacctt gcaaaaatac tcaaacaagc
     5161 cgggggtgac aacaaagcaa ttgaaaaagc gattgatgag ttaagaggtg gagaaacggt
     5221 taatgatcca aatgcagagg aacagcgaca agcccttgaa aaatatacac tggatctgac
     5281 tgaacgtgcc gagcaaggaa aattggaccc agtgattggg cgagatgatg aaatcagacg
     5341 caccatccag gtattgcaaa gaagaaccaa aaataaccct gtattaatag gtgaacctgg
     5401 tgttggtaaa acagccatag tagaaggttt ggcacaacga attattaatg gtgaagtacc
     5461 tgaaggatta aagaataaac gcttactggc actggacatg ggtgccttga tcgcgggtgc
     5521 aaaatatcgt ggagagtttg aagaacgttt aaaaggtgtg cttaatgatt tggcaaaaca
     5581 agagggtcaa atcatattat ttattgatga attacacact atggttggcg ctggcaaggc
     5641 tgaaggagct atggatgccg ggaatatgct caagccagca ttggctagag gtgaattgca
     5701 ttgcattggg gctacgacat tagacgaata ccgacaatac attgaaaagg atgctgccct
     5761 tgaacgacgc tttcaaaaag tactggtcga tgaacccagt gtggaagaca ctatcgctat
     5821 attaagggga cttaaagaac gttatgaagt tcaccatgga gttgaaatta cagatcccgc
     5881 tttggttgct gcagcaatgc tatcccatcg ttacatcagc gaccgtcagc tacctgataa
     5941 agccattgat ttaattgatg aagccgccag cttaatccgg atggaaattg attcaaaacc
     6001 agaaagtatg gataaactgg aacgacgttt gattcagcta aaaatcgagc gtgaagcatt
     6061 gaaaaaggaa aatgatgaag cgtctaaaaa aagacttgtt gatttacaaa aaagtatcga
     6121 tgaattagag caaaattatt cagacttaga agaaatttgg aaagctgaaa aagcgaccat
     6181 gcaaggttcc actcaaatca aagaagcact tgagcaagcc aaactggaaa tggaaactgc
     6241 aaggagagca ggagatttaa gtcgcatgtc cgaattacaa tatgggcgta tacccgagtt
     6301 ggaaaaaaga ttaagccagg tctcttccgt cgatgctatg gaaacaaagt tggtacgcaa
     6361 taaagtcacc gaagatgaga tagcagaagt tgtttcaaaa tggacaggga tccccgtatc
     6421 taaaatgatg gaaggagaaa aggaaaaact gctgaaaatg gaagaagctt tgcacagcag
     6481 actcattggc cagaatgaag cggtggatgc tgtttctaat gccataagac ggtcacgcgc
     6541 aggcctgtct gatcctaacc gtccaattgg ttcatttctt tttctggggc caactggtgt
     6601 gggaaaaact gaactatgta aagcacttgc ttcttttctt tttgatacgg aagaggcgat
     6661 ggtgcgtatt gatatgtctg aatttatgga aaaacactct gttgcgcgct taatcggagc
     6721 gcctcctggc tatgtaggat atgaggaggg tggttattta actgaagcag tcagacgcag
     6781 gccctattct gttatcttac ttgatgaagt agaaaaagct catacagatg tatttaatat
     6841 tctgcttcaa gtgatggatg atggccgtct aacagatggt cagggacgta ctgttgactt
     6901 taggaatacc gttattgtta tgacctctaa tctgggatct caactcattc aggaaatgag
     6961 cagcaaattt aattacgatg agattaaggc tgcggtaatg gatttagtca gccaacattt
     7021 cagacctgaa tttatcaaca gaattgatga atcggtggta ttccattccc tgacaaaaga
     7081 acaaattgca aaaattgcag caatccagat taactattta catcatcggc tgaaacaaca
     7141 aaacatcact cttgaagtca caagtgaggc tttatctcat ttggctgaag caggatttga
     7201 tccggtctat ggtgcccgcc ctctaaaacg tactattcag caaaaattag agaatcccct
     7261 agctcaatca ttattgactg gaaaatttaa atcaggggat accattattg tatcttataa
     7321 agacggagta atggaatttt ccaagcaatg agtatgctta tcattggata acaacgctgg
     7381 agtccttatc caggactcca aaattacgag gcaacgtttc aagaccaagc tgggtaaaca
     7441 catcaaacaa ataaagacgt agtgcgagtc tctgaattat cttcctgagc tttgtttact
     7501 gtttgaactt ttttagctaa agccaattct gtttgaagaa taaatgtatt cattgatcct
     7561 tttccaccaa aaaaatcaaa attaagctca actttaggat tagcttcaaa atatgtttta
     7621 agaagcctga ttgcgtccag catatcctta tcagtcaact cattgggatt tatttccaca
     7681 cccagagcct ggtgaatgca aaaccgtaat tgctccttta cgccctcgtt ttgtaataac
     7741 aaacaataac cacccaaagc ccgatatctt gcaagactgt tattacaatt tttcattgct
     7801 tcaataaacg aaccacaatc catcgtgcta aaattttcct gaatatgtcc ggtatacaac
     7861 agatcaataa ttttttcctg taaaacgttc gattcaattt gagcttttat ttgctcttcc
     7921 atcagttcca aggatatggt tttaaaatca gaacaggttt ttggcaagag cttgcctaaa
     7981 tgagtcaaag cctcttcaat ttgacgttgc tcttcttcaa tttgttttac caaggattga
     8041 atgaaattta tggccttgaa ttgatttaaa acaacagggt tacgtctttt gtgttcatca
     8101 ataatttctt gtaaataggt ttcaaaattc ttgtctgctg caaaatgagg atagctttta
     8161 tagcgcggca ttttcccctc atccttccct acaatcaatt gcatattatc acgaaatgtt
     8221 tccaaagcag ttacaatcgt tgttacatca agaattttca agtcatttat tctataattc
     8281 ctctccatca cctcaggtaa acctaaagcg agtcttattg cttgaaataa tcgacagtct
     8341 tttacattag atggtggctt actaaataaa
//
LOCUS       DATNSN010000140         8358 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.140, whole genome shotgun sequence.
ACCESSION   DATNSN010000140 DATNSN010000000
VERSION     DATNSN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.140"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            28..252
                     /locus_tag="VAH76_002497"
     CDS             28..252
                     /locus_tag="VAH76_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387094.1"
                     /translation="MSKFLVFIGSILFFSSVLAGDIPEVDIQDQQSDQELCAQKFYNQ
                     CINKCEKTNYGDCTQACEENAKNQCLYAGE"
     gene            381..953
                     /locus_tag="VAH76_002498"
     CDS             381..953
                     /locus_tag="VAH76_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO1387095.1"
                     /translation="MLHNTKINKLAVFCGSNTGYSEVYKQSAENMADVLSSNGITLVY
                     GGSKSGLMGIIANRMLKNGSNVIGVIPKSLVDTEKAHDGLTELHVVNSMYERKVLMCK
                     LSDGFILLPGGSGSLDEFFEMFTLVQLGYHKKPCCVLNASGYYDYLLQFLDYAVNQGF
                     LHSKDRNTLIVNQYPDTLIENLFEVINNRK"
     gene            993..1733
                     /locus_tag="VAH76_002499"
     CDS             993..1733
                     /locus_tag="VAH76_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO1387096.1"
                     /translation="MPKKLLIAAFLCNIFCNPSHLAYASSTEIPILGKTMTQAKQQQM
                     TPRQALQRLKDGNQRFLSNKPLARDYLKQAKQSAYGQYPFAVILNCMDSRSVPEFFFD
                     QGLADLFTLRVAGNVLNDDILGSMEFATKVVGARLVVVLAHTSCGAVAGACKDVKLGH
                     LTDVINKIQPVVKPSMESTGIDNCSDPKLIDDMAKANALHVVKNILEQSPILNELVKS
                     KQIGIVAGIHDIKTGKVTFFEEKRSVPE"
     gene            1830..3050
                     /locus_tag="VAH76_002500"
     CDS             1830..3050
                     /locus_tag="VAH76_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEO1387097.1"
                     /translation="MPKSHNFIIFLGLLSAFSLLTFDLYQPSLPFITNYFNITPRMGQ
                     LTLSIYLLVYGITHLLWGPLIDHFGRRRLLPGNLLLALIGSLMCAFAPNITMLICGRA
                     LQGFALCCANLIAYSAARDFEDTIVRAKVLSYISMTVSVSPIIAPVIGALIFHHFGWQ
                     ANFIVMAIVACILLIQTKLSLLESPFWTPPQERFSVKKILREYKSILNIPSLWCASFI
                     LMFGISAVMLTVINSSYLIIEVLNYSPLAYGLIFILNGLNIIFGNYLGIWLRDRLPII
                     TTIYLGNWFIVLGGTAMLLTSKLFGFSLLALSFSLIANLGISVSAAPTISIALTDFKQ
                     NAGIVMAFMNTIRLIGPSLLTILTGYLLTRNLDALPLGLIGSGIGALFFSWHFSRLTT
                     ESRSSDMDSKEAVT"
     gene            3296..6124
                     /gene="ceg32"
                     /locus_tag="VAH76_002501"
     CDS             3296..6124
                     /gene="ceg32"
                     /locus_tag="VAH76_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HEO1387098.1"
                     /translation="MTKIYLLTAPFDEEAKTGDGQYAASLELGFAENYEHIPCKWLKK
                     DKGDYSIPFPTGTTSIPEETIPSAMVLQPVANENTIISGYKLKDAVSSPEKAQEVNNK
                     TVSPRTPKIIVKHDNSLQSLTIMDIYSQKAIQFDESKVDEIINSLETKKDKLEKAIED
                     NNVDLSKIKKQKSKLAYLTRLYKENKENIQDNCTLNEYIEAHLFNPKFLSRHEKALNN
                     FKALKSQFTGPVNLKELEKLTDKLTGIKEYSYDFHSNSLPYDLEHDKSFRNFYDFDGL
                     KESIENIIKELEVLNGIRQAVSDKYPNCFKALNETEEHDDKLKFINIIFNDGFSTTYD
                     QQTFIKALSALDVEKAIDAYTNVKKKLENTQDIIANKEGGRNKLISELQTLIANKQEP
                     YLSANEKLGGFYSKRKLSASEGFHLAYQANRRDPIKPEVIENIITKMKPIDEDTHLDI
                     HIRPPDCGVFITPEDIKKFQEAGIKVNITIHEYKQNYTRRYLQQYTHDLMRQANSVQF
                     FNATDRENAIIAATYGDCDKRNTTEPTGVAKKIREVGEDFDLDKYPVQKYDLKGKSGL
                     TVASQKLSTEPDHPLDVVAKAPNILSFGTIRPGKGFEEALKLAQLIKDNSLSIDEKIK
                     RVPIVKLAGDPQDKALMKQIVIERFGKTAVKTYQKTHPYDNQFNNSQRRDYWKNLVRE
                     LNAKVKEEGAVLNNPYIEIYPWCEPHELLALKQNCKYVCRMDDMGMRNNGSAIISVLD
                     VGVVYTKFGSVTDDIFIKGGKYGNAVDIGEYRYGKYSLLKKEKEFKEQHEEEPLPKWL
                     IKNPDSAYKRQSESRDPKDILDSIVAREENQLICDNIEDSDNYRTVVEAQKLLKERFT
                     LKNAVDHLLENIGLGHLIAQEEVDELFETVDPVQAQIDNLDVLSDIKTPRLCLSRSCP
                     ELGFFGSRRGDLETKQENNKLKESILVF"
     gene            6126..7016
                     /locus_tag="VAH76_002502"
     CDS             6126..7016
                     /locus_tag="VAH76_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5630 domain-containing protein"
                     /protein_id="HEO1387099.1"
                     /translation="MVKGKLMPNFMKDVQKLICDEGITDNVIMTLSRLKPFDLAMLKA
                     TSDDKVKTLLDSDELKPFWVNKFNKLRLEKDHTFQFRNPDSQSRADFYCGYVLYLAAL
                     KEKQKEVSSYYDYLNLSFSTFNCFYAAQEILTFLIGACKNDSKRENIDLLYNSVTSQS
                     TQIQEHKTPGCLLLANAYFYLAGFYQRQDLKAESIECYKECWEQLHLAQLLETDSERE
                     IHNAYFSKGLATSNAFGLNSISEIKARCLELASEALPYPVRNVIEANAVKTFESRFKN
                     SMSMAGKEDEEENMPRPTIL"
     gene            7422..>8358
                     /locus_tag="VAH76_002503"
     CDS             7422..>8358
                     /locus_tag="VAH76_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO1387100.1"
                     /translation="MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFW
                     KLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLKFLPVFWYIVLLFCLPYFFAYLT
                     LINNGSTLWLMNCVSAIFFLLLVSSVLGALILLISGVGLAFFHFYILSNNQFVYIPGT
                     ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAE
                     LQELIVERLNNPEVQKDLKENLSKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYSI
                     KKLLKASLEEYPFKENQKSLIHLNDKNDFSIWIDEV"
BASE COUNT         2793 a         1471 c         1493 g         2601 t
ORIGIN      
        1 attaaataaa ccagggaaaa cgcgatcatg agcaaattcc tcgtatttat tggatcaata
       61 ttattttttt cttccgttct agctggagat atcccagaag tagacataca agatcaacag
      121 tcggatcagg agttatgcgc ccaaaaattt tataatcaat gcatcaacaa gtgcgaaaaa
      181 acaaattatg gcgactgtac tcaagcctgt gaggaaaatg ctaaaaatca atgtctttac
      241 gcaggtgaat aagaatggtt tgcgttgagg caattaaatt aaatcctata aattttatag
      301 tgaaaacatt aacttattaa aaataaactg tatttcgtca tttcatgtta taaataaaac
      361 attgcatctg gaattttaaa atgctccata ataccaaaat aaacaaactc gctgtctttt
      421 gtggatctaa cactggttat tctgaagtct ataaacaatc agctgaaaac atggccgatg
      481 tcctaagcag taatggtatt accctggttt atggtggctc aaaaagcggt ttgatgggaa
      541 taatagccaa tcgcatgtta aaaaatggct ctaatgtcat tggtgttata ccaaaatctc
      601 tggttgacac agaaaaagcg catgatgggt tgacggaatt acatgttgtt aattctatgt
      661 atgaaagaaa agtattgatg tgtaaattgt cggacgggtt cattctgctg ccaggtgggt
      721 caggttctct ggatgagttt tttgaaatgt ttaccctggt acaattggga taccataaga
      781 aaccttgctg tgtactcaat gcctctggtt actatgatta ccttttgcaa tttttagact
      841 atgctgttaa tcaaggtttt ttacactcaa aggatcgaaa cacgctcatt gtcaatcaat
      901 atccagatac actcattgag aatttatttg aagtaatcaa taatcgcaaa taacagttta
      961 ggaaaattaa taacataaca aggagttaac caatgccaaa aaaacttctc atcgctgctt
     1021 tcttatgcaa tattttttgt aaccccagcc atctggccta tgcctcatca acagaaatac
     1081 ccatacttgg gaaaaccatg acccaggcta aacaacaaca aatgactccc aggcaggcat
     1141 tgcaacgttt aaaagacggc aatcaacgct tcctaagtaa taaaccttta gcacgagatt
     1201 atctaaaaca agccaagcaa tcagcttatg gccaatatcc ttttgcggtc atcttgaatt
     1261 gtatggactc gcgcagtgtt cctgaattct tttttgatca aggattggca gacctgttta
     1321 ccttacgtgt tgccggcaat gttctcaacg atgacatttt aggaagtatg gaatttgcaa
     1381 caaaagtagt gggagcacgt ttggtagttg tcctggctca cacctcctgt ggtgctgttg
     1441 caggggcgtg caaagatgtt aaattagggc acttgactga tgttataaat aaaatccagc
     1501 ctgttgtaaa acccagcatg gaaagcacag gtattgataa ttgctctgat cccaaactga
     1561 ttgatgacat ggctaaagcc aatgccttgc atgttgtaaa aaatatacta gagcaaagcc
     1621 ctattttaaa tgaattagtt aagagtaaac aaattgggat tgtagcgggc attcatgata
     1681 ttaaaacggg taaggtgact ttttttgaag aaaagcgatc tgtcccagag taaagcaagt
     1741 tggcaattgc caacttgctt taatatttcc tttcaaatgc aagctgaatt tggttacaat
     1801 cgcataattt ttggtgaatg attttttata tgcctaaatc acataatttc attatatttc
     1861 ttggtttgct ttctgcgttt tctttgctta catttgatct ttatcaacca tcactgcctt
     1921 tcataacgaa ttattttaat attaccccac gtatggggca attaacccta agtatttatt
     1981 tgctagttta tggtatcact catcttctat ggggaccact tatagaccat tttgggcgcc
     2041 ggcgcctact tcctggaaac cttttattag ccttaattgg cagtttaatg tgtgcttttg
     2101 cgccgaatat caccatgctt atttgcggtc gtgccctaca aggattcgcc ttatgctgcg
     2161 caaatctcat tgcctattcc gcagcacgtg attttgaaga cacgattgtt cgcgccaaag
     2221 tgctgtccta tatttccatg accgtttcag tttcaccgat tatagccccg gtaattggtg
     2281 ctcttatctt tcatcatttt ggctggcaag ccaattttat agtcatggca atcgttgcct
     2341 gtattctatt gatacaaacc aaactctcct tgttagagtc ccctttctgg acgcctcccc
     2401 aggaaaggtt ctcggtcaaa aaaatactcc gggaatataa gtccatactc aatataccct
     2461 ctctatggtg tgcatcattt attttaatgt ttggcatttc tgctgtcatg ctcactgtaa
     2521 ttaattcctc ttaccttatt atcgaggtac tgaattactc tccacttgcc tatggactta
     2581 tttttattct gaatggttta aatattattt ttggaaacta tctgggcatt tggcttcgtg
     2641 atcgattacc cattataacc accatctatt tggggaactg gttcattgtc cttggtggta
     2701 cagcgatgtt gcttacttcc aaattgtttg ggtttagtct gttagcatta tcattttcat
     2761 taattgccaa cttgggaatt agtgtcagtg ccgctccgac tatctccatc gccctaacgg
     2821 attttaagca aaatgcagga atagttatgg cttttatgaa taccatacgt ttaatcggcc
     2881 cctctctttt aactattcta accggctatt tattgacaag gaatcttgac gctcttccac
     2941 taggattaat aggttcagga attggagccc tttttttctc atggcatttt agccgattga
     3001 ctactgaatc gaggagctcg gatatggata gcaaagaagc ggtgacctaa caacctgttc
     3061 cccccatatg cttaattact tttgatggat ctaacctcat attaatactc tgaattatga
     3121 ataatggctt ttttcaagtt ctatttatgc aattaagtca aaaaaattca taattttatc
     3181 aacatattat catctttcca ttccttatta tattttgcga ttatagctct taatgttcat
     3241 ttaataatct taatgtaaga tattcaccat gacaaaaatt ttactaaagt aatttatgac
     3301 taaaatatac ttattaaccg caccttttga tgaagaggca aaaacaggtg atgggcaata
     3361 cgcggcctct ttagaattgg gttttgctga aaattacgaa catatcccct gtaaatggtt
     3421 aaaaaaagat aaaggtgatt acagtattcc attcccaaca ggaactactt ccataccgga
     3481 agaaacgata ccaagcgcga tggtattaca gccagttgct aacgaaaata ccatcataag
     3541 cggttataaa ttaaaagatg ctgtttcgtc tccagaaaaa gcacaggaag taaataacaa
     3601 aacggttagt cccagaacgc ccaaaattat tgtaaagcat gataacagcc tgcaatcact
     3661 tacaataatg gatatttact ctcaaaaagc tatccaattt gatgagtcaa aagttgatga
     3721 aataattaac tctttagaaa caaaaaaaga taaactggaa aaagctatag aagataataa
     3781 cgttgatctg agtaaaatta agaaacaaaa atccaaatta gcgtatctga ctagattata
     3841 taaagaaaac aaagagaata ttcaagataa ttgcacctta aatgaatata ttgaggcaca
     3901 tttatttaat cctaaatttt taagcaggca tgagaaagca cttaataatt ttaaggcgct
     3961 taaaagccaa tttactggcc ctgtcaattt aaaagaactg gaaaaattaa cagataaact
     4021 gactggaatt aaagagtaca gctatgattt ccatagtaac agtttaccct atgatttaga
     4081 gcacgataaa agtttcagaa atttttatga ttttgatgga ttaaaagaat ctattgaaaa
     4141 cattatcaag gaattagagg tacttaacgg cattcgtcag gctgtatcag acaagtatcc
     4201 caattgcttt aaagcgctaa atgaaacaga agagcacgac gataaattaa aatttatcaa
     4261 tattattttc aacgatgggt ttagcaccac ttatgaccaa caaaccttta tcaaggcctt
     4321 atcagctctt gatgttgaaa aggcaattga cgcttataca aatgttaaaa agaaactgga
     4381 aaacactcag gatattatcg ccaataaaga ggggggtaga aataagttaa tttcagaatt
     4441 acaaacttta atagcaaata aacaagaacc ttacctctct gcaaatgaaa agcttggcgg
     4501 tttttacagt aaaagaaaac tctctgcaag tgaaggattt catttagcct atcaggcaaa
     4561 ccgacgtgac ccgataaaac ctgaagtgat tgaaaacatt attaccaaaa tgaaacccat
     4621 tgatgaggat actcatcttg atattcatat tcgcccacca gattgcgggg tctttattac
     4681 tccagaagac atcaaaaaat ttcaagaggc tggtattaaa gtcaatatca caatccatga
     4741 atataaacag aactacactc ggcgttattt acaacaatat actcatgatt taatgcgcca
     4801 ggcaaacagc gtgcaattct ttaatgcaac agatagggaa aatgctatta ttgccgctac
     4861 ttatggtgat tgtgataagc gcaatacaac tgaaccgact ggtgttgcaa aaaaaataag
     4921 agaagttggg gaagattttg acttagataa atacccggtt caaaaatatg atttaaaagg
     4981 aaaatcagga ttaaccgttg catcacaaaa attaagtacc gaaccggatc atccattgga
     5041 tgtagtagcc aaggccccta atatcttatc ttttggtacg attcgtcctg gcaaagggtt
     5101 tgaagaagcg ttaaagctgg ctcaattgat caaagataac tctctttcta ttgatgaaaa
     5161 aataaaacgg gtacctattg taaaactggc tggtgaccca caagacaaag ctttaatgaa
     5221 acaaatagtt attgaaaggt ttggtaaaac agcggtcaaa acttatcaaa aaactcatcc
     5281 ctatgataat caattcaata atagtcagcg cagagattat tggaaaaatt tggttaggga
     5341 gttaaatgca aaagttaaag aagaaggcgc tgttcttaat aatccttaca tcgaaattta
     5401 tccttggtgt gagccgcatg aattgctggc tttaaagcaa aactgtaaat acgtttgtcg
     5461 aatggacgac atgggcatga gaaacaatgg ctcagcaatt atcagtgtat tagacgttgg
     5521 agtcgtttat actaaatttg gttctgttac agacgatatt ttcattaagg gcggcaaata
     5581 cggcaatgcc gttgatattg gagaatatcg ttatggtaaa tacagtctgt taaaaaaaga
     5641 aaaggaattt aaagaacaac atgaggaaga accactacct aagtggctaa tcaaaaatcc
     5701 agactctgct tataagcgac aatcagaatc gagagatcca aaggatattt tagactccat
     5761 tgtcgcaaga gaagaaaatc aattaatatg tgataatata gaagattccg ataattatcg
     5821 aacagtagtc gaagcacaaa agttattaaa agagcgcttt acactcaaaa atgctgtaga
     5881 tcatttatta gagaatatag gactcggcca tttaattgct caagaagaag tcgatgaatt
     5941 atttgagaca gttgatccag tgcaagcaca aatagataat ttagacgttc tttctgatat
     6001 taagacacca cgtctgtgtt tatcacgctc ttgccctgag ctaggttttt tcggctcacg
     6061 tcgtggtgac ttagagacta aacaagaaaa taataaactt aaagaatcga tactggtatt
     6121 ttgatatggt aaaaggaaaa cttatgccca attttatgaa ggatgtgcaa aaattaatct
     6181 gtgatgaagg catcacagat aatgtgatta tgactctatc caggttaaaa ccatttgatt
     6241 tagccatgct caaagccact agtgacgaca aagttaaaac gctactggat agtgatgaat
     6301 taaaaccatt ctgggtaaat aaattcaaca aattgcgatt agaaaaagat catacttttc
     6361 aatttagaaa cccggattcg caatcaagag ctgatttcta ttgcggatat gttttatatt
     6421 tagcggcatt aaaggaaaaa caaaaagaag tcagtagtta ttatgattat cttaatcttt
     6481 ccttctcaac atttaattgt ttttatgcgg ctcaggaaat tttgacgttc ttaattggtg
     6541 cttgtaagaa tgattccaaa agagaaaata tcgacctttt atacaattct gtaactagcc
     6601 aaagcactca aatacaagaa cacaaaacac cagggtgctt attacttgct aatgcgtatt
     6661 tttatcttgc tggattttat caacgccagg atcttaaagc agaaagcatt gaatgctata
     6721 aagaatgctg ggaacaactt catttggcgc aattgttaga aacagattct gaacgagaaa
     6781 ttcacaatgc ctactttagt aaaggattag cgaccagtaa tgcatttggt ttgaattcca
     6841 tatcagaaat taaagctcga tgccttgaat tagcctcaga agcattaccc tatccggttc
     6901 gaaatgtcat tgaagcaaac gctgtaaaaa cctttgaaag tagatttaaa aacagcatga
     6961 gcatggctgg taaagaagat gaggaggaga acatgcctcg accaactatt ttatagtaga
     7021 gtccgagaac agtcagctga tattcttctt atggcgttgt gttataacgc caaaaccaaa
     7081 caagatgctc ttccaggcct ctgtattttg cataggacaa ttcatgttat attaaagcgc
     7141 aataatatga gtttttactt atggaaaagt aatattagta ttaaaaaaca gggattatgg
     7201 tatccttggt atttgtttta attgggagca atattattta acttaagtcc aagaaaccgt
     7261 agaaatcata actctaattg gtagcatcca ttcagagttc accattgaag tactcataat
     7321 ttacaagtat ttctaatcct acactgactg ttcaatgttc aggactaata agattaatac
     7381 ccaaacaatc tcaaaatttt tactcagata attaaggaat catgcaaagg ttaaaaaata
     7441 tatttactgc aatttaccaa tatttactaa aagaaactga agatgcaagc caccaaatca
     7501 ctttatttgg catcgtcatg atgattaatt accctctttt cggagttttc tggaaactgg
     7561 agcattttca actgacagaa gaatttattt tacgcatcac tgcagccctc ctctgtgcct
     7621 gtttggcatt taatcaattt tggccaagac aattattaaa atttctacct gttttttggt
     7681 atatagttct tctcttttgt ttgccttact tttttgctta tttgactctc ataaacaatg
     7741 gctcgactct ttggctaatg aattgcgtat ccgctatctt ttttctttta ttagtttcca
     7801 gtgtattggg ggctcttata ttacttatat caggtgttgg acttgctttt tttcattttt
     7861 atattttatc aaataatcag tttgtctata tcccaggaac catatcactt tttagtttaa
     7921 ttgttacgtt tattgcagca ataatcattg gtgccttgtt tgcacgagat agggaaatta
     7981 cctatgccgg acgtttatcg ggtatgcgca tgctggctgg aagtatcgct catgatttaa
     8041 gaacgccgct tgccagtatt tatttacaag cagaattaca ggaattgatt gtcgaaagat
     8101 taaataatcc agaagttcaa aaggacctga aggaaaattt aagcaaaatt actcgcggca
     8161 tagaaatgag taatcaatta attcgtatgc aattaaataa tattcaacga gacaaattag
     8221 ataccagtac attttcgatt tattccatca aaaaattgct caaagcgtct ttagaagaat
     8281 accctttcaa agaaaatcaa aaatcattga ttcatttaaa tgataaaaat gatttttcca
     8341 tatggattga tgaagtgg
//
LOCUS       DATNSN010000141         8299 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.141, whole genome shotgun sequence.
ACCESSION   DATNSN010000141 DATNSN010000000
VERSION     DATNSN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.141"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(9..1214)
                     /locus_tag="VAH76_002504"
     CDS             complement(9..1214)
                     /locus_tag="VAH76_002504"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO1387101.1"
                     /translation="MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTA
                     PDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAK
                     TYQHQVFKILDPDKTTVAFNSQWLNKFDAVDLIRLAATHTVARMLERDDFNKRYTTGQ
                     PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLI
                     EGLDGVKKMSKSLDNYIGINETPEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQ
                     SVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGNIPEDLEELSLVIAE
                     PIALVQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRI
                     AKLSIQQAD"
     gene            1471..2904
                     /locus_tag="VAH76_002505"
     CDS             1471..2904
                     /locus_tag="VAH76_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO1387102.1"
                     /translation="MDKRPNIPKRKSGNSSKLLAFFALVFAFSLPYLLVKNFSHPKRS
                     SYADQTDLYPELSDELSRDIPAEASTEIPHEKPDIKSATKPMQTKQDQQDKPINTSHV
                     KKDNEWQIVNPHSGDSMATIFHRLGLTAQNLNDVIKNNPHAKMLTRIKPNQKLQFLIK
                     KNKLEKLIVPVNTIQTLTVYRAGKIYKTKIDSKKTTTQNRYVTATVQGSLYTTAQRFN
                     IPSKLVRQMTTIFNKEIDFSRSLRSGDQFSIIYDAYYIEDKMVGVGDILVVTYTNHGK
                     TYQAIRHTNAHGEQDYFTPQGTSFKKAFTRYPIKFSHISSTFALSRYHPILHYRRAHK
                     GIDLAAPIGTPIHATGDGVITIIDRHNGYGNMIKIKHDKTYSTVYGHLLKFQKGLSKG
                     SRVKRGQVIGYVGQTGLATGPHCHYELHVNNQPRNPTTISLPTAAPVPAREMAAFKAK
                     ASTLLARLKLFEQAQLASKGKKKINVG"
     gene            complement(3035..3568)
                     /locus_tag="VAH76_002506"
     CDS             complement(3035..3568)
                     /locus_tag="VAH76_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HEO1387103.1"
                     /translation="MNGFANHLSNWLFLTFIVGIPLYAAIKKVNVFDAFIIGAKQGFD
                     VSVKLIPYLVAMMVAIGMLRASGFFSLMSQILSPALTMIGMPSELLPLALIRPFSGSA
                     STGVMAELIHKYGGDSFIAKTAATMMGSTETTFYVIAVYFGSVGIRRTRHAIPAGLLA
                     DLAGIIASVLICRYLFT"
     gene            complement(3565..4170)
                     /locus_tag="VAH76_002507"
     CDS             complement(3565..4170)
                     /locus_tag="VAH76_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HEO1387104.1"
                     /translation="MLNLIWLGMILISVVVGVIEGRIDEVVRAVTDSAKLGFELALGL
                     AGIMTFWLGIMSVATESGLVSILGKVLKPVLKRLFPDIPVEHPAMGAMVMNIAANMLG
                     LANAATPFGLQAMKELQSLNQNLKVATNSMCTFLAINTSSVQLIPVTAIAFLAANGST
                     NPSSVVFTSLIATTVSTLVAIVAVKQLAKLPRFKIERTDSL"
     gene            4240..5310
                     /locus_tag="VAH76_002508"
     CDS             4240..5310
                     /locus_tag="VAH76_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HEO1387105.1"
                     /translation="MKHTHNPFRYLIYFVFFITSLSSYSSQTTNIKEQTDTGLAPPAP
                     SITTNSAAIEEQANSSIRKLYHTLNTMPNTSMADRISQISAYFKGTKYILGSLGEGPN
                     ARYDQFPRYRVDGFDCDTYVNTVLSLALANSLESFQECLKHTRYKNGKRSYINRNHFT
                     SIDWNNYNQKRGLLKDITFSIRNEKKQPVALYANALINKPQWYNHKTIDTIRLQKQDK
                     NEQEKRLVELKAKGKTLETSLSNVPYIPFTALFSENKPNLHLFSQIPNGAVIEIIRPN
                     WDLRQQIGTELDISHLGFAIWINNELFFRQASSQYGKVVDVSLIDYLDKARSSPTIKG
                     INIQIVLPEKPVSNGCQLFDIR"
     gene            complement(5552..5845)
                     /locus_tag="VAH76_002509"
     CDS             complement(5552..5845)
                     /locus_tag="VAH76_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEO1387106.1"
                     /translation="MTQYNFERWSEMAKKIQEPFQAIAELNVKTLQGLSYLKPDELAQ
                     VKKPEEMLEKQINLAVENGHKALDYMQKSFQIIEKAMLGFVQEAKKTTAEVKK"
     gene            complement(6052..6450)
                     /locus_tag="VAH76_002510"
     CDS             complement(6052..6450)
                     /locus_tag="VAH76_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate biosynthesis repressor
                     PhaR"
                     /protein_id="HEO1387107.1"
                     /translation="MTRLIKKYKNRRLYDTEISQYITVEQLQKYVVDGIDFKIEDSET
                     GKDLTNSVLLQIIVEMEAGPTQFLSSEILRQIISLANHPMHVSFKEMMEQMFHAMDKP
                     LQNNPYQQATEVWNKQMQQMVHQWQKMFKV"
     gene            complement(6580..7326)
                     /locus_tag="VAH76_002511"
     CDS             complement(6580..7326)
                     /locus_tag="VAH76_002511"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO1387108.1"
                     /translation="MLNNNVVLITGGTGDIGTAVAKQLDSTFKHVIALDLVSYDEGEV
                     WLHDLKTQGYKNINFRHMDVTDYEQCAEVIGLIIEEFGGIDVLINNAGITRDAVFTKM
                     TKRQWDEVLRVNLDGMFNVTKQVVESMKAQESGRIVNVSSVNAQKGQFSQANYAASKA
                     GVYGFTKSLAQELMIKNITVNSISPGYVNTRLMKGIRPDILEGIINLIPAKRLAEPQE
                     IAWAIEFLISEKSRYITGANLSVNGGLHMY"
     gene            complement(7504..8244)
                     /gene="phbB"
                     /locus_tag="VAH76_002512"
     CDS             complement(7504..8244)
                     /gene="phbB"
                     /locus_tag="VAH76_002512"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEO1387109.1"
                     /translation="MDKMIAIVTGGTGGIGSAISQRLADSYQVVACYYKDGRHEEAKK
                     WQDEQKQLGYDIDIVYGDIAQYSDCEKITSLVMERYGRIDVLVNNAGITKDCSLRKMT
                     PQQWQQVLDANLTSVFNMTRNVVPVMLERGYGRIVSISSINGRKGQFGQCNYASTKAA
                     LFGFTKSLALEVASKGITVNTVSPGYIETPMLAALKEDVLNSIISSIPVGRLGYPKEI
                     ADAVAFLASPDSGFITGANLDVNGGQYM"
BASE COUNT         2697 a         1728 c         1298 g         2576 t
ORIGIN      
        1 aaattatctc agtctgcttg ttgaatactc agcttggcaa ttcgacgttt tccaacttga
       61 ataatataag acttgccaat aggtaatttt agggaaggat ctgaaatttt atccccatcc
      121 actttaacag ccccttgttt caccattctt atagactctg atgtgctggc tgtcaaatca
      181 atttgcttta ataattgtac taaagctatc ggctctgcaa taactaacga tagttcttcc
      241 aagtcttcag gaatatttcc tttttgaaat cgttcaataa acttgttatg agcgaattcg
      301 gcctgagttt gatcatgaaa tcgcgcaaca atttctttgg caaaatcaat cttaacatct
      361 ctgggatttt tcccttctaa tacagattgt tttaattgtt gtatttcctt gcctgtttta
      421 aagcttaata aatcaatata tcgccacatt aactcatcag aaactgacat aatttttccg
      481 aacatttgct caggtgtttc attgatccca atgtaattat caagggattt cgacattttc
      541 tttacaccat ccaaaccctc tattaatggt gtcatcatga caacttgagg ctcaaagcca
      601 taatgcttct gtaattctct acccatcaat aaattaaatt tctgatcagt cccgccaagt
      661 tcaacgtcag cttttaatgc caccgaatca taaccttgta acagcgggta caaaaattcg
      721 tgaattgcga taggctgtcc tgtagtataa cgtttattga aatcatctcg ttccagcatt
      781 cttgccacag tatgtgttgc agccagtcga atcaaatcaa ccgcatcaaa tttattaagc
      841 cattgtgaat tgaaagccac agtagtctta tcagggtcaa gaattttaaa tacctggtgt
      901 tggtaggttt ttgcattttc cagaaccgtt tcctgactca atggcattcg ggtgacattt
      961 tttccagtcg gatcaccaat catggctgtg aaatcaccaa tcaaaaaaat aacctcatga
     1021 ccaaattgct gaaactgcct caatttatta agcagtacag tatgtcccaa atgaagatct
     1081 ggcgctgttg gatcaaaacc tgcctttatt ttcaatggga tacctttttg taatttcttc
     1141 tccaactcag gtacaggaag aatttcctcg cacccccgca ttaattcgga acaaactgaa
     1201 tcttgaacta tcatgtcatt aaaacccgta taaatgattg acctatcttc tcgcagcgag
     1261 tatcttagcc cggttagcaa tttcctgcta taccaaatga tacattttga gataatttat
     1321 tactctaaat tgagaaaaat ataacaaaat atccaaatat gagtcgattt agttgactaa
     1381 aggtagcggt cacttccaaa taatgactgt gatatattga ttgaaattaa aatagcagca
     1441 tttttcagat gacggcaata taggcaaaag atggataaaa gaccgaatat accaaaaaga
     1501 aaatctggta actcatcaaa gctattagcg ttttttgcgt tggtatttgc cttttcgctt
     1561 ccgtatttgc tagtgaaaaa tttttcacac cctaaacgaa gcagttatgc tgaccaaact
     1621 gatttatacc ccgaactatc agacgagtta tcaagagata tacctgctga agcttccacg
     1681 gaaattccgc atgaaaaacc tgatattaaa tctgctacaa aacctatgca aacaaagcaa
     1741 gatcagcaag acaaaccaat caatacaagt catgtaaaaa aagataatga atggcaaata
     1801 gtcaatcctc attcaggaga ttctatggcg accattttcc ataggctagg actaaccgcg
     1861 caaaatttaa atgatgtaat aaaaaataac ccgcacgcca agatgcttac aagaattaaa
     1921 cctaaccaga aattacaatt tttaattaaa aaaaataaat tggaaaaatt aattgtccct
     1981 gtaaacacca ttcaaacact aacagtttat cgcgctggga aaatatacaa aaccaaaatt
     2041 gactcaaaaa aaacaaccac tcaaaatcgt tatgtgactg caacagtcca aggttctttg
     2101 tatacaacag cccaacgatt taacatcccc tcaaaattag tccgtcaaat gaccactatc
     2161 tttaacaaag aaattgattt ttccagatct ttgcgctccg gagatcaatt ctcgataatt
     2221 tatgatgctt attacattga agataaaatg gttggagtag gagacattct ggtagtaacg
     2281 tatactaatc atggaaaaac ttatcaagca atacgtcata ctaacgctca tggtgagcaa
     2341 gactatttta ccccacaagg caccagcttt aaaaaagcct tcactcggta tccaattaaa
     2401 tttagccata taagctcgac ttttgcttta tcgagatacc acccaattct tcattacaga
     2461 cgagcgcaca aaggtatcga tttggctgcc ccgattggaa caccgataca tgctaccggc
     2521 gatggggtta ttacaatcat cgataggcat aacggatatg gcaatatgat taaaattaag
     2581 catgataaaa catacagcac tgtttatggg catttactca aatttcagaa aggtctatca
     2641 aaaggcagta gagtaaaacg aggtcaagta attggttatg taggacaaac cggtctggca
     2701 accggacctc attgccatta tgagcttcat gtaaataatc agccaagaaa cccaactaca
     2761 ataagcttgc ctactgcagc accagtacca gcaagagaaa tggcggcatt caaagcaaaa
     2821 gccagtaccc ttttagcccg gctaaaactt ttcgagcaag cgcaattagc aagtaaaggg
     2881 aaaaagaaaa ttaacgttgg ataaaagctg cgcgtgggat ttatccatac ccaaatacct
     2941 aacttaaatg ttagcagaca cttctttata aagcgagtaa gctccaagta attcaaacat
     3001 aacgttactc tttttgtatt aaaaaaatct tatatcatgt aaataaatat cgacaaatta
     3061 aaaccgaggc aataattcca gctaaatctg ccaataatcc agcaggaata gcatgtcttg
     3121 tccttctgat acccactgaa ccaaaataaa cggcaatcac ataaaaagta gtttctgtac
     3181 ttcccatcat tgttgcggcg gtttttgcga tgaatgaatc tcctccgtat ttatgaatca
     3241 gctctgccat aactcctgtt gaagcacttc cagagaaagg tctgataaga gctaaaggta
     3301 ataattcgga aggcattcct atcatagtca acgctggaga taagatctga ctcattaaag
     3361 aaaaaaaacc tgaagctctt aacatgccaa tggcaaccat catcgcaact aaataaggta
     3421 taagctttac gctaacatca aaaccttgtt ttgctccaat tataaaagca tcaaatacat
     3481 ttactttttt aattgcagca tacaaaggga taccaacgat gaaagtcaaa aacaaccaat
     3541 tggataaatg attagcgaat ccattcataa gctatctgtc ctttcaattt taaatcgcgg
     3601 taatttagcc aattgtttta cagcaacgat agctaccaga gtagaaacag tagtcgcgat
     3661 caaagaagtg aagaccacac tacttgggtt agtgctcccg tttgcagcaa gaaatgcgat
     3721 agcggtgacc ggaattaatt gcacactgga agtgtttatt gccaagaaag tacacatgga
     3781 attcgtggca actttcaaat tctgattaag gctctgcaat tctttcattg cctgcaatcc
     3841 aaagggggta gcggcattag caagacccaa catgttcgca gcaatattca ttaccattgc
     3901 tcccatagca ggatgctcaa caggtatatc aggaaataat cgctttaaca ctggtttgag
     3961 tactttacct aaaatagaaa ctaaacctga ttctgtagca accgacatta tccctaacca
     4021 gaaagtcatt attccagcca aaccaagcgc taactcaaat cccagcttgg cagagtcagt
     4081 aactgctctt actacctcgt caatacgccc ctcaataact ccaaccacca ctgaaatcag
     4141 tatcattcct aaccatatta gattgagcat tattgcctcc aacctctgaa aaaggttaat
     4201 atgtaaccaa aattagattt aaaataccaa tcaggactta tgaaacatac tcacaatccc
     4261 ttcagatact taatatattt cgtttttttt atcaccagcc tttccagtta tagcagccaa
     4321 acaaccaaca ttaaggaaca aactgataca ggtttagccc cacccgcccc ttcgattacc
     4381 actaactcag ctgctattga agaacaggcg aattcttcaa ttaggaaact atatcataca
     4441 ctcaatacca tgccgaacac ttctatggca gatagaatta gccagatcag cgcatatttt
     4501 aaagggacaa aatatattct tggctctttg ggcgaaggtc caaatgctcg ttatgatcaa
     4561 tttcccagat atcgagttga tggctttgat tgtgatacct atgtcaatac agtactttct
     4621 ctagcattag ctaactctct tgaatcattc caggagtgtt taaaacatac tcgctataaa
     4681 aatggaaaaa gatcttatat caacagaaat cattttacct ctatagactg gaataactat
     4741 aatcagaaga gaggtttact taaagacatt accttctcta tcagaaatga aaaaaagcag
     4801 cctgttgctc tttatgctaa tgccttaatc aacaaaccac aatggtataa tcataaaacg
     4861 atcgatacca ttcgattgca aaaacaagat aaaaatgagc aggaaaaacg tcttgtggaa
     4921 ctaaaggcca aaggtaaaac gctggaaact tctctttcta atgtccctta tattccgttc
     4981 actgccttat tttcagagaa taaacctaac ctgcatttat tttcccaaat tcctaacggg
     5041 gctgttatcg aaatcatcag acctaattgg gacttacgcc aacaaatagg gacagaatta
     5101 gatatttcgc atttaggctt tgctatttgg attaataacg aactgttttt tcgacaagcc
     5161 tcttcccaat atggtaaagt agttgatgta tccttgattg attaccttga taaagcacgc
     5221 agtagcccaa ctataaaagg gattaatatt cagattgtat tgcctgaaaa accagtatct
     5281 aatgggtgcc agctttttga tattcgatag cttataaatt aacatcttat ggttaaccta
     5341 taattttcgt aattaaaccc gtataaccat taattatttt atgaaatggt cgatgttttt
     5401 attaacgaga tactcgtgtt tcgtaataaa aaatcgattt ctattttaat tgactcgctt
     5461 ggctattatc tggaagttgg tggctggaaa tatgctcaat gatatgaaaa tagaaaaggc
     5521 ttattttgat caaataagcc ttttaaagga attatttctt tacttcagca gttgtttttt
     5581 tagcttcttg cacaaaacct aacatggctt tttcaataat ttgaaatgat ttttgcatat
     5641 aatccaaagc cttgtggcca ttttccactg ccaaattaat ttgtttttcc agcatttctt
     5701 cgggcttttt aacttgagcc agttcatcag gcttcaaata acttaaccct tgcagggttt
     5761 taacattcag ttcagcaatg gcttggaaag gctcttgtat tttttttgcc atttcactcc
     5821 atctttcaaa attgtattgg gtcataatat tctccatttg ttgcactgca caatataata
     5881 gtagacctta tctttattat ttgtcaatat cttatctaat tattttttgc agtgcaccaa
     5941 aaatcacgat tatattcatt gatttttgat gtgtattgca ctttccaaat catcagtaaa
     6001 attcgtaaag cgaaacattc aatttactac aaaaaagttt gggaggaatt tttagacttt
     6061 aaacattttt tgccattgat gtaccatttg ttgcatttgc ttattccaga cttcagtcgc
     6121 ttgctggtaa ggattgtttt gtaaaggttt atccattgca tgaaacattt gttccatcat
     6181 ttccttaaag gaaacatgca ttggatgatt agctaatgaa atgatttggc gtaaaatttc
     6241 tgaagacaaa aattgcgttg gaccagcctc catttccact ataatttgta acagaaccga
     6301 attagttaaa tcctttcctg tctctgaatc ttctatcttg aaatcaatac catccacaac
     6361 atacttttgt agttgctcaa cggtaatata ttgactaatt tcagtatcat atagtcttcg
     6421 atttttgtat ttttttatga gtcgagtcat tattttctca agcatcagat atagaaatat
     6481 aattataaaa tgatgtgcaa aattggtata gtttgcttct ttaagcccaa gagtaaatct
     6541 ttcacacttc ttttacaaat tctttcaagg ttaaaaaagt taatacatat gcaaaccacc
     6601 attgacacta agatttgctc ccgtgatata tcgacttttc tcactaatca gaaattcaat
     6661 agcccaagca atttcttgtg gctctgccaa tctttttgcg ggtatcaaat taataatccc
     6721 ctccagaata tctggtctaa tacctttcat cagacgagta ttaacgtaac caggagaaat
     6781 actgtttact gtaatatttt taatcattag ttcctgggca agacttttgg taaaaccata
     6841 tactccagct tttgaagcag cataattggc ttgggaaaat tgccctttct gagcattaac
     6901 cgaagacaca ttcactattc ggcctgactc ctgagctttc atactctcta ccacttgttt
     6961 agttacatta aacattccat caagattgac acgcaacact tcatcccatt gccttttggt
     7021 cattttggta aacacggcat cgcgagtaat tccagcatta ttaatcagca catcaatacc
     7081 accaaattct tcaataatta agcctattac ttcagcacat tgctcataat cagttacatc
     7141 catatgcctg aaattaatat ttttatagcc ttgtgtcttc aaatcatgaa gccatacttc
     7201 accctcatca taggaaacca aatccaaagc aatgacatgt ttaaaagttg aatccagttg
     7261 ttttgcaaca gccgtaccaa tgtcaccagt tccaccagta attaatacca cattattatt
     7321 cagcatgaat ttctctatta gtttttccta tccccaattg atgatttaaa attggagctt
     7381 atattttatt cattgttaac ggtgatcgca gccacttttt caaaatccca aggtgccttg
     7441 cgaacagtaa ataatctgtt cacaaaacct cttgagattc ttaatctgcg aatcaaactc
     7501 tgactacata tactgaccac cattgacgtc cagatttgcc cccgtaataa atccgctatc
     7561 tggtgatgct aaaaatgcaa cggcatctgc aatttctttt gggtatccca atcgaccaac
     7621 aggaatactg gatataattg aattgagtac atcttctttc aatgcagcca gcataggagt
     7681 ttcaatatac ccaggcgaca ctgtgtttac cgtaatacct ttacttgcta cttctaatgc
     7741 aagactcttg gtaaaaccaa ataaagcagc cttggtggaa gcataattgc attggccaaa
     7801 ctgccctttt cttccattga ttgaagatat actgacgata cggccataac ctctttccaa
     7861 catcactgga acgacattgc gcgtcatatt gaatacactg gtcaagttgg cgtctaaaac
     7921 ctgttgccat tgttgaggtg tcatttttct taaactgcaa tctttagtaa tgcctgcgtt
     7981 gttaaccaaa acatctatac ggccataacg ctccatcacc agggatgtga ttttttcgca
     8041 atcactatat tgagctatat ctccatatac gatatcaatg tcataaccaa gttgcttttg
     8101 ctcatcttgc catttttttg cttcttcatg tcttccatct ttatagtagc atgccacaac
     8161 ttggtaacta tcagctaagc gctgactaat agcagaacca ataccgcctg ttcccccagt
     8221 tacaatcgct atcattttat ccatcgacaa ctccttttgt tgctaggtaa cattacatta
     8281 ctacaatcta atttcactt
//
LOCUS       DATNSN010000142         8185 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.142, whole genome shotgun sequence.
ACCESSION   DATNSN010000142 DATNSN010000000
VERSION     DATNSN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.142"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..845
                     /locus_tag="VAH76_002513"
     CDS             <1..845
                     /locus_tag="VAH76_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HEO1387110.1"
                     /translation="ALLLSPLLAFANSDNNADGLIFSPLPQNKNTVVRHYSNEQDMSN
                     LNQMTQRTIDFPTQIVRVSGNLTGLELSCDDVENEIDQIFSKKISPNLFTYNTYVSCG
                     FDEHDPEQYATNFSIQSYFDPLTDNAVDYLKNYLKEYNGYNLFNTTTLQIENAKGIIV
                     SMNLNAGLKNNPDKTPFMLYRQDRNNFYFKSNFDMRKELITDIYQRFYSNDPDMILPF
                     FDKWIFSYAGSVYYSILKASNYLELQPERIFVMENEGDIFVSDLRYYFANLCMKRNPN
                     KHCL"
     gene            complement(1042..2094)
                     /locus_tag="VAH76_002514"
     CDS             complement(1042..2094)
                     /locus_tag="VAH76_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEO1387111.1"
                     /translation="MLKGLKVIDLSTVLAGPSVGTFFAELGASVVKIEHPVHKDVTRT
                     WKLPGEDPHSEISAYFASVNFGKDYLFLDLMKEEDYLCFLNLIAEADILIMNFKKGDD
                     IKLRIQADHLLSINPRLIIGKINGYGEESERVAYDLVLQGESGFMSINGTPDSGPVKM
                     PVALIDVLAAHHLKEAILLALYNREKSGKGKVVSVSLYDAAVSSLVNQASNYLMTGQV
                     PQRIGSLHPNIAPYGELFRTADAALIILAIGSDRHFAKLCAVLGLNDLPDNDEFKHNQ
                     NRVRNREKLALLIAEQTAKWQSNKLLELLHSENVPAGKIMDLKDVFDDERAKSLIRKE
                     LIGDIPTARVTSVVFK"
     gene            complement(2233..3642)
                     /locus_tag="VAH76_002515"
     CDS             complement(2233..3642)
                     /locus_tag="VAH76_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO1387112.1"
                     /translation="MIRKKITQISFFMILALIVGTVEAKTTRPADEQQLARSIYKQFI
                     EVQSGFTTGSTTPLVEVAVKYLKKAGFSNEDIFVGGASPQKANLVVRYRGTGEMKPLL
                     LLAHTDVVEAKASDWSMNPFQLTEKDGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKP
                     KRDIIVALTADEEGSSPYNGISWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQV
                     SEKYIVNYNLEVRNKGGHSSLPTKDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQM
                     AAIETGPLKTEMIEASKGSKQAMQRLANSSPQWNAILRTTCTPTLLEGGHAMNALPQL
                     AAVTINCRVLPEDSPEMVEQSLKTAINDPEVTLKRIGKLSRGPSSPLSPEILKTITHL
                     TQTYWPEVPAIPIMVTGATDGRYLRSAGIPTYGVMGLFLDRDDFRAHGRDERISVESF
                     YEAHAFLYDLVKQLSSNVT"
     gene            complement(3902..5380)
                     /locus_tag="VAH76_002516"
     CDS             complement(3902..5380)
                     /locus_tag="VAH76_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1387113.1"
                     /translation="MNIAIIGGGTGLTTAWALENQRTFHVTVFEENDRLGGHIHSIKI
                     NNVILEGGAEFIGPPALYPNVHRLFQYLGMALDQFELNMDFDNLKQRDHIVLPPLYHT
                     SSGKSEDVSSILYTLSCGLFGKRRKQNETRISVDTMLTEMYNLLEMNHLIMDARNKLL
                     HPDNLMTLEQFIKEFKEKCVHVLSPVDKFAEEFVYPLIASGWGVSIETIKKFGAHYAM
                     NYLAAGMNWFDAPEGLSSYIDKMVAQCTNTQFKVNTAIKKLIPITIDGKIKYQLLKRD
                     DTFVCDATGSPIIYDDVVISTPAYVTNELLSDIESNTIKVLREKLANVTYYDTTVVFH
                     QDPEYLSPYRTVVHSRFDGTQSANTMCKPWKFNKDEIPIMKTWVLPGQEMPKNVLREV
                     HYKHPVMDKNYYEAQQALHAAQGEAGLWHGGILAGFNDSHESGVTAALQVAAQLNQRE
                     HCLDKNERLALFPHILDSVLSSKLSTDMRVEVAAVAAYPSMP"
     gene            complement(5585..6496)
                     /locus_tag="VAH76_002517"
     CDS             complement(5585..6496)
                     /locus_tag="VAH76_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387114.1"
                     /translation="MYSKYPVFFPNKNIKSFSGVRFSNVVGIPSAIESLYRGDNNLAD
                     IIFLLPTLITGVFCQNFPEVVDMEQVRLHKLTNLSNDFHMVSMSEDPQIALDWGKGCF
                     ITIDPVLFSDYIVDVHATFRKNQLNFPDRMEQEREHIALAVPFCSIEKITIHNQELAN
                     PFYLSIPQDNHEARMEFNTLYSELISLLRKKYTQEADGEEEKVALRAYAIRYLDFYSK
                     FGSCNNPFNKSLAQLVELYPEFMSNFLQSSHLSSKTGLMKDVVLNSSENLFKEHPYTQ
                     SIDASYIFRVKESTTCYEDDWAKPVYD"
     gene            6698..7540
                     /gene="htpX"
                     /locus_tag="VAH76_002518"
     CDS             6698..7540
                     /gene="htpX"
                     /locus_tag="VAH76_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO1387115.1"
                     /translation="MINNLKTFILLASLTALLVVIGGLLGGSTGMLVALLFAGIMNFS
                     AYWYSDTLVLKMYNAEPLSNNHFVYHIVSELAHRAGTSVPKVYLINNSTPNAFATGRN
                     PENASIAVTTGLLDRLTQEEITGVLAHELAHVIHRDTLINVVSATIAGAISGIANMFM
                     WLSMFGHNSNNEEGVHPVVGMIMMIVAPLAAGLIQMAISRSREFEADAGGARISGNPQ
                     WLASALLKLDQANHEQYFDEAETHPATAHLFIINPLNGEKLANLFSTHPSTAERVARL
                     RAMY"
     gene            complement(7666..>8185)
                     /locus_tag="VAH76_002519"
     CDS             complement(7666..>8185)
                     /locus_tag="VAH76_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO1387116.1"
                     /translation="YMQADAENMAIANESFDIVTNLESSHLYPQIEHFFSEVERVLAP
                     NGFFCYADVNFDVKRQAERFETFVKTRKNLRIIEKHNITKMIQASIYQRLIVNEKRFY
                     QLAQYLLGPDKINYTSELANVASSMGLMFLPWWRIRFKNPALQALGKFARRYKHWNKK
                     YYFYYLIQKIDS"
BASE COUNT         2458 a         1693 c         1494 g         2540 t
ORIGIN      
        1 gcgctttgct tttatctccc cttctcgctt tcgccaattc tgataacaac gcagatggcc
       61 ttattttttc gccattgccg cagaataaaa ataccgtcgt aaggcattat agcaatgaac
      121 aggacatgtc taatcttaac cagatgactc aaagaactat tgattttcca actcaaattg
      181 ttcgtgttag cggaaatctt acaggattgg aactgagctg tgacgatgta gaaaatgaaa
      241 tagatcaaat cttttcaaaa aaaatttctc ctaatttgtt tacttacaac acctatgtca
      301 gctgcggctt cgatgagcat gatccggaac agtatgcgac aaatttctct attcagagtt
      361 atttcgatcc attgacagat aacgctgtgg attatttaaa aaactatctg aaagaatata
      421 atggatacaa tttattcaat actacaacat tacaaattga aaatgccaaa ggcatcattg
      481 tatcgatgaa tcttaatgcg ggcttaaaaa acaaccctga taaaacacca ttcatgcttt
      541 accgtcaaga taggaacaat ttttatttta aaagcaattt tgacatgaga aaggaattaa
      601 tcaccgatat ttatcaacga ttttattcta acgatcctga tatgattcta ccctttttcg
      661 acaaatggat tttctcctat gcgggttcag tttattactc cattctgaag gcatccaatt
      721 atcttgaatt gcaacctgaa agaatctttg taatggaaaa tgaaggggat atctttgtct
      781 ctgatttaag atattatttc gccaatcttt gtatgaaaag gaatcctaat aaacattgct
      841 tgtaaataat tcaattattg agccacgggc ttgtctttag gcgctccaca atcgtggctc
      901 ttaggattcc aggccatttt tgggcgcacc gttatgaaat cgcaatttgg agcagagttt
      961 gaatttgcat ttgaaatatt cgcttttctt gcatacaaaa tttaaaccct gcccacatta
     1021 gggttttaga taagtcctaa tctatttaaa aacaaccgat gtaacacgcg ccgtgggaat
     1081 atcgccgatg agttcctttc gaatgagaga tttcgcacgc tcatcatcga aaacgtcctt
     1141 gagatccata attttcccag ctggaacatt ttcactgtgc aacagttcga gaagcttgtt
     1201 actttgccat ttcgctgttt gttcagcaat taataaagcg agcttttctc ggttccttac
     1261 cctgttttga ttgtgcttaa attcatcatt atccggcaaa tcgtttagcc caaggacagc
     1321 gcatagtttt gcaaaatgcc tatcagaacc tatagccaag ataataagcg cagcatcagc
     1381 tgtacggaac aattcgccat agggggcgat atttggatgc agtgaaccga tacgctgcgg
     1441 cacctgccca gtcatcaagt aattagatgc ctgattgaca agactgctga cagctgcatc
     1501 ataaagtgaa acagagacaa ccttgccttt cccgcttttt tcccgattgt agagagccag
     1561 aaggatagcc tctttcaggt ggtgagctgc gaggacatcg attagagcga caggcatttt
     1621 aactgggccg ctgtcaggtg tcccattgat tgacataaaa ccgctttcac cctggagaac
     1681 aagatcatag gcaacgcgct cgctttcttc accataacca ttgatttttc cgataattag
     1741 gcgcgggttt atactgagaa gatgatcggc ttgtattcgt agctttatgt catctccctt
     1801 tttaaaattc ataattaaaa tatctgcttc tgcaataagg ttaaggaaac agagataatc
     1861 ctcttccttc atgagatcta aaaacaaata gtcctttccg aaattaacac tggcaaaata
     1921 agctgaaatc tcagaatgag gatcctctcc tgggagtttc catgttcttg tcacatcctt
     1981 atgcacagga tgttcaattt taacaactga agcacctaac tcggcaaaaa aagtgcctac
     2041 agagggccct gcaagaaccg tactcagatc aatgaccttt aagcccttca acatatatct
     2101 gttttccaaa tctcaataaa cattcgttgt ttttacaagc ttttacattg ggaaattctg
     2161 caaaactgac cctagggacc tcaaatcaat atcctgcctg atttagtgag gccccaactg
     2221 gaaaaatcat gcttaagtta cattgcttga cagttgtttc accaaatcat aaaggaatgc
     2281 atgagcttca taaaatgatt cgacactaat ccgctcatca cgaccatggg ctcggaaatc
     2341 atcgcgatct aaaaatagcc ccatcactcc ataggtagga attccagctg atcgcagata
     2401 acgtccatct gttgcgccag taaccataat tggaatggca ggaacttcag gccaataagt
     2461 ttgagttaga tgagtaattg tttttagtat ttccgggcta agtggagaac ttgggcctct
     2521 ggacaatttg ccaatccgct ttagtgtaac ctctggatcg ttaatagctg tcttcaaaga
     2581 ttgctcaacc atttcaggtg aatcctctgg gagcacacga caatttatgg taacagccgc
     2641 taattgtggt aaagcattca ttgcatgccc tccttcaagt aaggtagggg tacaggttgt
     2701 tcgtaaaata gcattccatt gcggtgaact attagctaaa cgttgcatag cttgcttcga
     2761 gcctttgctt gcttcaatca tttctgtttt cagaggccct gtctcaatag ccgccatttg
     2821 tttaaaatag gccgcagtga catcattggt ttttaaagga aaattaaatt ttgacagtcg
     2881 ctctaaagcc ccggcaagac gataaatggc attatctttg gttggcagcg aactatgccc
     2941 ccctttatta cggacctcaa ggttgtaatt gacaatgtat ttttcactca cctggatatt
     3001 ttgtgatatt tttttcccgt tagccaaatc accccatcca ccctcattaa gagcgaaatc
     3061 tgcctcgatt aaatccttgt gatttttaat cagccaacta atcccgttgt atgggctgct
     3121 tccttcttca tcagccgtca aggcaacaat gatatcgcgt ttgggtttaa atccttcctg
     3181 tttatattgg attaagttgg ctatccaaat agccgcttgc gctttatcat ccaaagtgcc
     3241 tctgccataa aaatacccat ctttttcagt cagttggaaa ggattcatgc tccaatcgga
     3301 agctttggct tcaacaacgt ccgtatgcgc aagtaataac aagggtttca tctccccagt
     3361 accgcgataa cgtacaacca gattggcttt ttgtggactg gctccaccaa cgaaaatgtc
     3421 ctcgttggaa aaaccggctt tctttaagta ttttacagcc acctcaacca gcggcgttgt
     3481 tgaaccggta gtaaatcctg attgcacttc gataaattgt ttataaatag agcgagcaag
     3541 ttgttgttca tccgcgggac gagtagtctt ggcttctaca gtcccaacaa tcaatgccaa
     3601 aatcataaaa aatgaaattt gagttatctt tttcctaatc ataaaatgaa atccattcaa
     3661 agaattaagc gtttaaagaa aaactcacta taaagtaatc tgagtaagaa tcaatgcgct
     3721 tttggagcca gagcttgaat cttgaatgcc aactgttcat tttccttgat ctgcagagtt
     3781 taaatctggc ctctattgtc gcaagaaaat ttaaaatcag atccacaatt atcgctctca
     3841 atttgtcctt gagagcgata tcaatttaat agaaattgcc aaagaataac cgatgtcagg
     3901 tttatggcat tgaaggatag gcggctactg ctgcaacctc caccctcata tctgtagaca
     3961 attttgaaga taggacagag tcaagtatat gagggaatag cgcgagacgt tcatttttat
     4021 ctaaacaatg ttctcgctga ttcaattggg cagcaacttg taatgctgct gtaacaccac
     4081 tttcatgtga atcattaaat ccagcaagaa taccgccatg ccataacccg gcctcacctt
     4141 gtgctgcatg caaggcttgc tgtgcttcgt agtaattttt atccatgacc gggtgtttgt
     4201 aatgaacttc tctcagtacg tttttaggca tttcttgccc aggcaaaacc caggttttca
     4261 ttatgggtat ttcatcttta ttaaatttcc atggtttgca catggtgttt gctgattgag
     4321 tcccatcaaa acggctgtgc acaactgttc tgtagggaga caggtattcg ggatcctgat
     4381 gaaatactac tgtcgtatca taataagtga cattcgcaag tttttcccgt agcactttta
     4441 tcgtgttact ttcaatatca gacagcagtt catttgtaac ataagccggt gtagagatta
     4501 caacatcatc ataaattatt gggcttccag tggcgtcaca aacgaatgtg tcatctcttt
     4561 tcaataattg atactttatt tttccatcaa ttgttattgg tattaatttt tttatagccg
     4621 tattcacttt aaattgagta ttggtgcatt gcgccaccat tttatcaata tagctgctca
     4681 aaccttctgg cgcatcaaac caattcattc ctgctgccag atagttcatg gcataatggg
     4741 cgccaaattt tttgattgtt tcaattgaaa ctccccatcc tgaggcaatc aaaggataca
     4801 cgaactcctc agcaaacttg tcaacaggcg agaggacatg aacacatttc tctttaaact
     4861 cttttataaa ttgttctaat gtcattaaat tgtctggatg cagtaattta ttcctcgcat
     4921 ccataattaa gtgattcatt tcaagcaaat tgtacatttc tgtgagcatg gtatccactg
     4981 aaatgcgagt ttcattttgt ttccttctct tgccaaacaa accacaagac aatgtgtata
     5041 aaatcgacga aacgtcctcg gactttccac ttgaggtatg atacaaagga ggtaaaacaa
     5101 tatgatccct ttgtttcaga ttgtcaaaat ccatatttaa ctcaaattga tctaaagcca
     5161 ttcccagata ttgaaacaat cgatgcacat tgggatacaa ggcaggtggt cctataaatt
     5221 ctgcaccccc ttcaaggata acattgttaa tcttgatact gtgaatatgt ccgcctaatc
     5281 gatcattttc ctcaaataca gtcacatgaa atgttcgttg attttccaaa gcccaagcag
     5341 tagttaatcc tgttccacca ccaattatcg caatattcat tactttttta gtccttttaa
     5401 aagtccattt ccaaatgcca ggctcgtaaa ctaaatggtt agaataaaaa agtaaagtgt
     5461 ctttctgtgg ttaaatttat aaaccctgag tctacccttc ttggcacatc ggtcttaatg
     5521 tacttctgtc aaaaactttt tccatatatg tgcattaact ccatttttct tattactttg
     5581 ttggctaatc ataaaccggt tttgcccagt catcttcata acaggtagtg ctctctttaa
     5641 ccctaaagat ataagaagca tcaatcgatt gagtataagg atgttcttta aataaatttt
     5701 ctgatgagtt caggacaaca tctttcatca aacctgtttt tgatgaaaga tgacttgatt
     5761 gcaaaaaatt gctcataaat tccggataga gttcaaccaa ctgagcaagg cttttattaa
     5821 atggattgtt acaactgccg aatttagaat aaaagtctaa ataacgtatg gcgtaagcac
     5881 gtaatgcgac tttctcttct tccccatcgg cttcctgagt gtattttttt cttaaaagtg
     5941 aaattaattc gctgtagagc gtattaaact ccattcttgc ttcatgatta tcttggggta
     6001 tgctgagata aaaagggtta gctaattctt gattgtgaat tgtaattttt tctatgctgc
     6061 aaaaaggcac agctaaagct atatgctctc tttcttgctc cattcggtca ggaaaattga
     6121 gctgattttt acgaaaagtg gcatgaacat ctacaatata atcagaaaat aaaacagggt
     6181 cgattgtaat aaaacatcct ttcccccaat ccagagcaat ttgaggatcc tctgacatac
     6241 tcaccatatg aaaatcatta gataggttag ttaatttgtg caatcttact tgctccatat
     6301 ccacaacctc aggaaaattt tgacaaaata ctcctgtaat tagagtaggc aaaagaaaaa
     6361 ttatgtcagc aagattatta tcacctcgat ataatgattc aatggcagag ggaattccta
     6421 caacatttga aaaccgcaca cctgagaaag actttatatt cttatttggg aaaaatactg
     6481 gatatttact atacataaga tgtttaatga catataaatt tgcgcaacaa tataccacat
     6541 tgatctcaaa ataaaaataa ccgcgtatta cgatcaatgg gttttcttga tctccagctc
     6601 taaaacatgg acataaacag agataacgct ccaagttgaa tttttaacat ctggccttat
     6661 atatgaaata taggaagagt caatgaggtg atgagaaatg ataaataacc tgaaaacttt
     6721 tatcttgcta gcctctctaa cagcattgct agttgttatt gggggattac ttggcggttc
     6781 tacaggaatg ctggttgcgt tactattcgc aggaattatg aatttttcgg cttactggta
     6841 ttcagatact cttgttttga aaatgtataa tgctgagcct ctttctaata atcactttgt
     6901 ttaccatatt gtttcagagc ttgctcatag agcaggaacc tcagtaccca aagtttattt
     6961 aattaataac tctactccga acgcgtttgc aacggggaga aatccagaga atgccagtat
     7021 cgctgttaca acaggattgt tagataggct cactcaagaa gaaatcaccg gagttttagc
     7081 tcatgaactg gcccatgtaa tccaccgcga cactttaatt aatgtggtaa gcgcaacaat
     7141 agcaggggcg attagcggga ttgccaatat gttcatgtgg ctttcaatgt ttggccacaa
     7201 ttcaaataat gaagaaggtg tccacccagt ggttggcatg atcatgatga tagtagctcc
     7261 cctggcggct ggactaattc aaatggctat atcgcgctcc agggagtttg aagcggatgc
     7321 cggtggagca cgaatttccg ggaatccgca gtggctggcc agtgccttgt taaagcttga
     7381 tcaagccaac catgagcaat actttgatga agcagaaact caccctgcca ctgcgcacct
     7441 gtttatcatt aatccattaa atggagaaaa gctggcgaac ctcttttcaa ctcatccctc
     7501 aacagcagag cgggtagcaa ggctaagagc catgtactaa ttgaggtttc tctcattgat
     7561 ttggaataag catcgcttta atcacagcca taaacatagt actccatcct cgttatactc
     7621 agtgcatctc aataaaacca tgtactggaa acagtggatt ggtttttatg agtcaatctt
     7681 ttgaattaaa taataaaaat aatatttttt attccagtgc ttgtaccggc gagcaaattt
     7741 tcccagcgct tgcaatgccg gattcttaaa gcgaatcctc caccagggaa gaaacatgag
     7801 tcccattgaa ctggccacat tagccagctc cgaagtatag ttaatcttat caggccccaa
     7861 aaggtattga gccaattggt aaaatctttt ttcattgaca attaaacgct gatagattga
     7921 tgcctggatc attttagtaa tattatgctt ttcaatgatg cgtaaatttt ttcttgtctt
     7981 cacaaaagtt tcaaatcgtt ccgcttgccg cttcacatca aaattaacgt ccgcataaca
     8041 aaaaaatccg ttaggcgcta aaaccctctc tacttcagaa aaaaaatgct cgatttgagg
     8101 ataaagatgt gaactttcaa ggttagtgac aatatcaaaa ctttcgtttg ctatagccat
     8161 attttctgca tcagcctgca tataa
//
LOCUS       DATNSN010000143         8175 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.143, whole genome shotgun sequence.
ACCESSION   DATNSN010000143 DATNSN010000000
VERSION     DATNSN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.143"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..808
                     /gene="csy3"
                     /locus_tag="VAH76_002520"
     CDS             <1..808
                     /gene="csy3"
                     /locus_tag="VAH76_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HEO1387117.1"
                     /translation="QTVDTCSLGSEEDTLKLYFTLKVLGGLKHPSACNNAAFKQSYQQ
                     AVSNYLETKGMRELAKRYALNIANARFLWRNRFGAENIQVEVMALNEGAEKSWNFNAL
                     DFNPRDLETTTNEVDSLADRIADALASENDFLMLQITCYARIGKAQDVYPSEELVLDK
                     GKGKKSKILYAVDGIAAMHSQKIGNALRTIDTWYPEFSDPVSSAGPIAIEPYGAVTNL
                     GKAFRIPADKQDFYTFFDTWARGGELSRVEDEHYMMAVLVRGGVFGESDK"
     gene            819..1373
                     /gene="cas6f"
                     /locus_tag="VAH76_002521"
     CDS             819..1373
                     /gene="cas6f"
                     /locus_tag="VAH76_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HEO1387118.1"
                     /translation="MDHYLDISILPDSEFTTPVLMNAIYTNLHKALHTLASTNIGVSF
                     PKYSSTLGNLLRIHGKKEALQELQNLNWIGGMIGYCEASLIKTVPADTKFRTVSRKQP
                     TMSQSKLRRLIKRNSLTEDEIRQYKAKMFSKGLDNPYIELVSVSNGQRHRRYIEFGEL
                     FNEPIPGLFDQFGLSNSATVPWFD"
     repeat_region   1605..6319
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1605..1632
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            complement(6835..7401)
                     /locus_tag="VAH76_002522"
     CDS             complement(6835..7401)
                     /locus_tag="VAH76_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO1387119.1"
                     /translation="MSSSGKTIGYVRVSSFDQNPDRQLEGIILDKKFIDHISGKNIKR
                     PQLEKLLDYVRDGDEVVVHSMDRLARNLDDLRQLVKSLTQRDVTLRFIKENLVFKGDD
                     SPMSNLLLSIMGAFAEFERALIRERQLEGIAIAKQKGKYRGRKPSLNKDQAAILQERA
                     KAGEQKTSLAKEFGISRETLYQYLKNNQ"
     gene            complement(7630..8061)
                     /locus_tag="VAH76_002523"
     CDS             complement(7630..8061)
                     /locus_tag="VAH76_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387120.1"
                     /translation="MSHIPLSNYGTSPFEKLLGYVPEILNNWSNLENAFLQSKTFEPN
                     FLEQIRRALVFDNLCYYYIAKARPPDQRPEDILLTAALKFANKFAIDHKIINEQDIDE
                     LKQCFLTNEISELISYCSFITASQRFGSVLGLQPALKYDQK"
BASE COUNT         2543 a         1610 c         1693 g         2329 t
ORIGIN      
        1 tcaaaccgtt gatacttgtt cattgggttc agaagaggac acgcttaaat tgtattttac
       61 gcttaaggtg ctaggcggat taaaacatcc ctctgcttgt aataatgctg cttttaaaca
      121 gagttaccag caggctgtaa gcaattatct tgaaacaaag ggtatgaggg agttggcaaa
      181 acgttatgcg ctgaatatcg ccaatgcccg atttttatgg cgcaatcgct ttggagcaga
      241 aaatattcag gtggaggtta tggcattaaa cgaaggcgct gaaaaatcct ggaattttaa
      301 tgcacttgat ttcaatccac gtgatttgga aacaaccact aacgaagttg acagtttggc
      361 agatcgtatt gctgacgcat tggcaagtga gaatgacttt ttaatgcttc aaatcacttg
      421 ttatgccaga attggcaaag ctcaggatgt ttatcccagt gaagagttag tgctggacaa
      481 gggtaaagga aaaaaaagca aaattcttta tgccgtagat ggtattgcag caatgcattc
      541 gcaaaaaatt ggcaatgctc ttcgaacgat agatacctgg taccctgaat tcagtgatcc
      601 ggtatcttct gctggaccaa tcgcaattga gccatatggt gcagttacca atttgggaaa
      661 agcgtttagg ataccggcag acaagcagga tttctatact ttttttgata cttgggcacg
      721 tggcggagag ttatctcgcg ttgaagatga gcattatatg atggctgttt tagtgcgtgg
      781 tggtgttttt ggtgaaagtg ataaataggg ggtaggttat ggatcattat ttagatatat
      841 ctattcttcc cgattctgaa tttacaacgc cagtgttaat gaatgcaatt tacaccaatc
      901 tccacaaagc cttacatact ctggcttcca ctaacatcgg tgttagcttt ccaaagtaca
      961 gctctacttt gggaaacctt ttacgtattc atggtaaaaa agaggctttg caagaactgc
     1021 agaatttgaa ctggataggc ggtatgattg gttattgtga ggcaagcttg ataaaaacag
     1081 ttcctgcgga cacaaaattc cgtacggtgt ctcgtaaaca gcctacaatg agccaatcaa
     1141 aattgaggcg gttaattaaa cgtaactcct tgacagaaga tgaaatcagg caatacaagg
     1201 caaagatgtt ttccaaaggt ctggataatc catatattga acttgtgagc gtatcaaacg
     1261 ggcaaaggca ccgacgctat attgagtttg gcgaattgtt caatgagcca attcctggcc
     1321 tctttgatca gttcggcctt tctaacagtg ctactgttcc atggtttgat taaattttag
     1381 aacaaggtaa attccttgag ttcgataggt taaagtaagg agatatagtt tggtcaaatg
     1441 cttcaatact tgattatttc gatacttcta acgaccaata aatttagttc tttaaaaatt
     1501 tgtcttaaaa tcaattggtt ataaattagc tgaaagatat tgggtaaatc gatttttttc
     1561 agctaacttt ctgttataac tattttttta agatttagag cagagttcac tgccgtacag
     1621 gcagcttaga aattaatgca tcttccataa gatggaccac tgaagttcac tgccgtacag
     1681 gcagcttaga aattttttag tttaaaaatt agttgattat aaatgttcac tgccgtacag
     1741 gcagcttaga aatttaaaaa tgcgtagagt ttctctagct catggttcac tgccgtacag
     1801 gcagcttaga aatgcagcct tcaaaatctc tttatgcgca aatggttcac tgccgtacag
     1861 gcagcttaga aaaacatttt ctgataagcc agagttatgt actgtgttca ctgccgtaca
     1921 ggcagcttag aaataatcat atttgcaaca cctattacag ctacagttca ctgccgtaca
     1981 ggcagcttag aaatataaaa tgaaaaggta tatggtcaaa atattgttca ctgccgtaca
     2041 ggcagcttag aaacataaaa aagtaatttc atcactttta ggatttgttc actgccgtac
     2101 aggcagctta gaaatgctgt aactcttaat agcaatattt ctcaaagttc actgccgtac
     2161 aggcagctta gaaaagcgaa aagcacagca acacgacaac aaggcagttc actgccgtac
     2221 aggcagctta gaaatgactg caaagctcct aggcgtttct aggggcgttc actgccgtac
     2281 aggcagctta gaaaaactgc ggtgaccatt atatgatttc atgattgttc actgccgtac
     2341 aggcagctta gaaataatta cgaggattaa acatgaacaa acaaatgttc actgccgtac
     2401 aggcagctta gaaaagttgg ggatatcgtg tggatggcgg ctcatagttc actgccgtac
     2461 aggcagctta gaaattgcag gtaggcacgg aaaaggagca gcaaaagttc actgccgtac
     2521 aggcagctta gaaatcctaa caaagaatta agcgcttttc caaggcgttc actgccgtac
     2581 aggcagctta gaaatgagga gtacaaaaag aggcacaaaa agggcagttc actgccgtac
     2641 aggcagctta gaaattggcc caaaaatggt accaattcaa gaaaacatgt tcactgccgt
     2701 acaggcagct tagaaagttg tggtaaacaa tttcatccgc ttttaaaagt tcactgccgt
     2761 acaggcagct tagaaactac gtgcagatag gcgaaggggg ggtatgcccg ttcactgccg
     2821 tacaggcagc ttagaaatta tattcaagta aatattaatt gcccactttg ttcactgccg
     2881 tacaggcagc ttagaaatca ccataggttt aaaaacctac aaaattaaag ttcactgccg
     2941 tacaggcagc ttagaaaatc atggcgtgaa gttgttcacg ctattgaaag ttcactgccg
     3001 tacaggcagc ttagaaatct gaagcatctt tgatgcaaga ccatttttag ttcactgccg
     3061 tacaggcagc ttagaaattg gcagcgttta agcatggctt ggagtacggg ttcactgccg
     3121 tacaggcagc ttagaaatta cacgctggac atctaatcat ttccatttgg ttcactgccg
     3181 tacaggcagc ttagaaaaaa ctcagcagct tcagcaaatt ctgggtgagg ttcactgccg
     3241 tacaggcagc ttagaaattg ctatgccata aattgccaat gcaacacaag ttcactgccg
     3301 tacaggcagc ttagaaactc tttgagagat attactgtta agagttacag ttcactgccg
     3361 tacaggcagc ttagaaataa aaagaacata gccagcctgc tagcccttag ttcactgccg
     3421 tacaggcagc ttagaaaatc gacacagcta cgaacaataa aataaaaagg ttcactgccg
     3481 tacaggcagc ttagaaatga gctggttcat tagttgtatt tggccaatag ttcactgccg
     3541 tacaggcagc ttagaaatac aagtacagtt tttagtcgtg gacgtagagg ttcactgccg
     3601 tacaggcagc ttagaaatac tattttattt agtgatgatt tagttaatgg ttcactgccg
     3661 tacaggcagc ttagaaaaga cccaacgcct gcaaccgatt gcactccggg ttcactgccg
     3721 tacaggcagc ttagaaattg ctgatagttc aaatcagcac gaacagaaag ttcactgccg
     3781 tacaggcagc ttagaaacat caccgaggtc aacaacaaca gagtcttgtg ttcactgccg
     3841 tacaggcagc ttagaaataa actgaccggt cacaaggcta ggtttataag ttcactgccg
     3901 tacaggcagc ttagaaatat gtacagtgtt ccgggtctta ttgatcactg ttcactgccg
     3961 tacaggcagc ttagaaataa ttacttagaa agtaacgcag aaaagatatg ttcactgccg
     4021 tacaggcagc ttagaaaaag tttcaaaaaa gttgctccac catcacaaag ttcactgccg
     4081 tacaggcagc ttagaaaaga tgaagtgcat tctgatttaa ttcgtgaagg ttcactgccg
     4141 tacaggcagc ttagaaacag caacaagagg cgcaactaaa cctaacacag ttcactgccg
     4201 tacaggcagc ttagaaaact accccatgtt gatctatagg agtattccag ttcactgccg
     4261 tacaggcagc ttagaaatag tgtcactaat tgcagagatt atacaattag ttcactgccg
     4321 tacaggcagc ttagaaaaca taaaaaaaat cttttaggaa gtatattatg ttcactgccg
     4381 tacaggcagc ttagaaaata ctttcacaaa attcacaaac atgatcatcg ttcactgccg
     4441 tacaggcagc ttagaaaaac agctccgtac acgttagagt tactgtctcg ttcactgccg
     4501 tacaggcagc ttagaaaaga taaaattgtc aatactttat atttaaattg ttcactgccg
     4561 tacaggcagc ttagaaaaaa acaatataga tgtactgtca atcataattg ttcactgccg
     4621 tacaggcagc ttagaaacta gtatgtaacg ttaaaccact gattttatac gttcactgcc
     4681 gtacaggcag cttagaaatg aacgagtacg gcgaaccaag atattattgc gttcactgcc
     4741 gtacaggcag cttagaaaag tgacatatta gaaaccatgg ctccaaagct gttcactgcc
     4801 gtacaggcag cttagaaatt caaatttgcg ttatagggca ttttcttttt gttcactgcc
     4861 gtacaggcag cttagaaaaa ttcagcataa aaagcatctt taacgttttg cgttcactgc
     4921 cgtacaggca gcttagaaaa aaaagaacat agccagttta ctagggctta ggttcactgc
     4981 cgtacaggca gcttagaaaa catataccag cagtaccagc agcatttgat ggttcactgc
     5041 cgtacaggca gcttagaaat atcagactta aacgaatctc actataaaac agttcactgc
     5101 cgtacaggca ggttagaaag gcgtataatt atctcgtatg tattgctcta agttcactgc
     5161 cgtacaggca gcttagaaaa aaattgtttt tctgctcaag agatggggaa cgttcactgc
     5221 cgtacaggca gcttagaaat atgatgacgg cctccacgga gaacttgtta tgttcactgc
     5281 cgtacaggca gcttagaaaa tcacgaatgc acccaaattg cctgttagtt agttcactgc
     5341 cgtacaggca gcttagaaaa tgtagcgaag gcgaggttcc ataatgtata agttcactgc
     5401 cgtacaggca gcttagaaac actcgatggg gttttcggag aatcattcag cgttcactgc
     5461 cgtacaggca gcttagaaaa ttaccatttt tttaatctgc tcagataatg ggttcactgc
     5521 cgtacaggca gcttagaaat tccagctcta atattttaca cgcatcatat tgttcactgc
     5581 cgtacaggca gcttagaaac tgcgggcttg gaaatgatga aggctgttaa tgttcactgc
     5641 cgtacaggca gcttagaaat gtctgccgtg tcgccgccgc cagaacctaa cgttcactgc
     5701 cgtacaggca gcttagaaaa attgaacaga tagttgaaga atcagtaaaa ggttcactgc
     5761 cgtacaggca gcttagaaat tcttagtccc tttacacgct gggcatctaa tgttcactgc
     5821 cgtacaggca gcttagaaat tgagagttgc tcttatggcc tcttttgtag agttcactgc
     5881 cgtacaggca gcttagaaat aattcatgaa cgatagggta ggtactagca tgttcactgc
     5941 cgtacaggca gcttagaaat cattttgaac tgtaaataaa cctttcgtta agttcactgc
     6001 cgtacaggca gcttagaaaa ctgtggtgag tatggtgaga agtacggaag agttcactgc
     6061 cgtacaggca gcttagaaaa tgtgcaccta caccgtgaat gtgccggtat tgttcactgc
     6121 cgtacaggca gcttagaaat ctgagcttga gcagcttgaa aaacaaatac agttcactgc
     6181 cgtacaggca gcttagaaaa aaacgtgctc caccatttag ttgacgggtc agttcactgc
     6241 cgtacaggca gcttagaaag cttgtgccat gcgcttaact attgcccttt cgttcactgc
     6301 cgtacaggca gcttagaagt tattatgtta aaggtgttag cacgctacgc agcaaggtcg
     6361 gaccaaggcc tgaccttgtt ttgattagga aagtctaatc atgactataa tctccatttc
     6421 tctttatgga gtacagcaac cgttagtcaa atctataaat aacctaaaca aataaaaata
     6481 aactctctag ttccatggaa atgagtgtaa ttggatcgaa gtcaaaataa attgtgaagt
     6541 ctgattactg agaaaattaa atttagttga tgagtatcta ttgaatcatg agaataatct
     6601 aatggtgtac gttaattata cacgtatgct aaaaattact acctgatgag ctaggatatt
     6661 ctctaccgcg ttgtttgaat cacactgagt caatcgtaaa tttatttagc attactgaga
     6721 attatttaat cctcaaaaac tcaataataa atttagatat ttcacaaaat catacggtga
     6781 atgctctcct ggtttcgtca gttactgaaa tagtggttag ttctgattag ttacttattg
     6841 attgtttttt aagtattgat agagtgtttc tctgctgatt ccaaattctt tggctaagct
     6901 agtcttttgt tcgccagcct ttgctcgctc ttgaagaatt gcagcctgat ccttatttaa
     6961 agagggcttc ctacctctgt atttcccttt ttgtttagca atggcaatac cttcaagctg
     7021 tctctctcta attaatgcac gttcaaactc agcaaaggca cccataatag ataaaagcaa
     7081 gttggacatg ggtgagtcat cacctttaaa taccaaattt tctttaataa aacgcagagt
     7141 cacatctcgt tgtgttaagc tttttacgag ttgcctcaaa tcatccaggt ttctggctaa
     7201 tcggtccatg ctgtgaacga ccacttcatc gccatcacgg acatagtcga gaagtttttc
     7261 cagttgcggt cgtttaatat ttttcccgga aatgtgatcg ataaattttt tatctagaat
     7321 aattccctcc agctggcgat ctgggttttg gtcaaaagag cttactcgaa catatccgat
     7381 ggttttacct gatgaagaca taaaaactcc atttctataa aatcgtcagg ataaagttta
     7441 agattttaaa ttgattgtgt caatttatta aaaattgagc tctattctga cataattttt
     7501 cactttgccc agtgtcaatt tagcgtatac cacaagatga caccaataaa atcccaataa
     7561 tctagcaatt cgaataatac aggcaatgaa aaacattttt tgtgatgaga tgattcttat
     7621 agctgaccat catttttgat catattttaa agctggctgt agacctaaca cggatccaaa
     7681 acgttgtgat gcggtaataa aagaacaata agaaattaat tcactgattt cgttagtcaa
     7741 gaaacattgc tttaactcat caatatcttg ttcattgatt attttatgat caatagcaaa
     7801 tttatttgca aattttaatg cggccgtaag cagaatatct tctggtcgtt ggtcaggagg
     7861 tcttgcctta gctatataat agtagcaaag attatcaaat actaaagcgc gtcttatctg
     7921 ctctaaaaaa ttaggttcaa aagtcttgct ctgcaaaaag gcattttcta aattagacca
     7981 attatttaag atctccggaa catagccaag cagtttttca aaaggagagg taccatagtt
     8041 tgatagtggg atatgggaca tttcaataga tggttaaaat tatcctttgg tctttttatc
     8101 actataacta tatacggtgc aagacaacta ttttaaaaat gacaaatata ctttaattgc
     8161 cgtccgcttt actaa
//
LOCUS       DATNSN010000144         8164 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.144, whole genome shotgun sequence.
ACCESSION   DATNSN010000144 DATNSN010000000
VERSION     DATNSN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.144"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            299..3127
                     /locus_tag="VAH76_002524"
     CDS             299..3127
                     /locus_tag="VAH76_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEO1387121.1"
                     /translation="MSEILEPVKDIPLTNQLELTANVPGMLKTIKRNGKVVIYDDTKI
                     KIAITKAFIADEGGTAPTSDRIHQQIEELTRQITQVFKRRLPSGGAIHIEEIQDQVEL
                     ALMRSGHYKVARSYVLYREERRKARESESKQKQTKDSKHLLITMPNGEVQPLDMDRVN
                     TIVKESCRDLSNVKAEPVIKDALRNLYNQAKYEDVHKALIMSARALVEKEPNYTYVSA
                     RLLLDSLRSEALTKLNIKSEATFDEMAQIYPVYFKSYIEQGIAQGMLDPKMADFDLDK
                     LSQALLPERDMKFTYLSLQTLYDRYFIHERGVRYELPQAFFMRVAMGLALREQHKEEK
                     AIEFYQLLSSFDYMSSTPTLFNSGTVRPQLSSCYLTTVPDHLDGIYSAIKDNALLSKY
                     AGGLGNDWTPVRAMGAHIKGTNGKSQGVVPFLNVADATAVAVNQGGKRKGAVCAYLEC
                     WHRDVEEFLELRKNTGDDRRRTHDMNTALWVPDLFMKRVREDGNWTLFSPDEVPDLHD
                     QYGKAFEALYVEYEEKARQGIIRNTKTISAVKLWRRMLSMLFETGHPWLTFKDPCNLR
                     SPQQHAGVIHSSNLCTEITLNTSEEEIAVCNLGSINLPAHIKNGKLDEQKLKRTISTA
                     VRMLDNVIDINYYSVPQARNSNLQHRPIGLGIMGFQDALYELKMDYASKEAIEFADSS
                     MELISYYAIEASCDLAKERGSYSTYEGSLWSKGILPIDSINLLQQSRNKYLEQDRSQK
                     LDWEPLRVKVRTQGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLSGEFTV
                     VNPYLVADLKALNLWDEVMVNDLKYFNGSVQPISRIPDELKRRYATSFEIDPMWLVEA
                     ASRRQKWIDQAQSLNIYMAQPSGKKLDQLYMHAWIRGLKTTYYLRSLGASNAEKSTVT
                     DGALNAVKLIDTEPKACSITDPDCEACQ"
     gene            3141..4244
                     /locus_tag="VAH76_002525"
     CDS             3141..4244
                     /locus_tag="VAH76_002525"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEO1387122.1"
                     /translation="MNYNDTQEIIVTPLMGATGLETLEMGAARIHVDDKQIINCRADL
                     NQLVPFKYKWAWDKYLTACANHWMPNEISMSADVALWKDPYGLTEDERLIIKRNLGFF
                     STADSLVANNLVLAVYRHITNPECRQYLLRQAFEEALHTHAYQYIIESLGLDEAEVFN
                     MYREIPSVSRKAVWALPFTQSLGDETFHTGTPENDQRLLRDLIAFYVVFEGIFFYVGF
                     TQILSMGRRNKMVGTSEQFQYILRDESMHMNFGIDVINQIKIENPHLWTPEFKNEMIQ
                     LIREGVELEYQYARDTMPHGILGMNAEMFEEYLHFIANRRLNQIGLPDQYPGAENPFP
                     WMSEMLDLKKEKNFFETRVIEYQAGGTLSWDDE"
     gene            4559..5044
                     /locus_tag="VAH76_002526"
     CDS             4559..5044
                     /locus_tag="VAH76_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1776 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387123.1"
                     /translation="MQSKIHKKEDNTNYSFLLKCMAAATAALATAAIVTAVVIKATAT
                     AAVGFGVAATGAALSPLLFVGGALLAAVGICCLPLLLCCGSSRSVYTVSNSPAYRPWS
                     SAWYTGGYNPTFHSHPVYNNGGTYHTHHSGTVHTHSNPVHTHGNNVHVHTTPSNVHGH
                     R"
     gene            complement(5131..6621)
                     /gene="lysS"
                     /locus_tag="VAH76_002527"
     CDS             complement(5131..6621)
                     /gene="lysS"
                     /locus_tag="VAH76_002527"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO1387124.1"
                     /translation="MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADAL
                     LKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDI
                     YEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
                     RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVT
                     HHNTLDMTMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQA
                     YADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIK
                     AQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISP
                     LARRSDTNPEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHF
                     DSDYIEALEYGLPPTAGEGIGIDRLVMLFTNSQSIRDVILFPHLRQ"
     gene            complement(6618..7725)
                     /gene="prfB"
                     /locus_tag="VAH76_002528"
     CDS             complement(join(6618..7649,7651..7725))
                     /gene="prfB"
                     /locus_tag="VAH76_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114835330.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO1387125.1"
                     /translation="MLEVNQINLSLIDLNERIESLRGYLDFEGKSERLEEVIRELESS
                     NVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEE
                     LKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHG
                     FTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     AVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQCQN
                     DRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIK
                     DLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA"
     gene            complement(7785..8156)
                     /locus_tag="VAH76_002529"
     CDS             complement(7785..8156)
                     /locus_tag="VAH76_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HEO1387126.1"
                     /translation="MIFLLLGNYLLNQRKNINKLQNKIDSLEKLATQVVKDHPMLINA
                     DLLFAKQLNEINQQLISIETQLQELENIRHNDGSYQHALRILEMGGTREEIVSSCHLS
                     NAEAELLMNLQAYRTALKHQN"
BASE COUNT         2527 a         1697 c         1496 g         2444 t
ORIGIN      
        1 aaatttaacc cccgcagagt attttaaccc acaacttatc cacagaataa tcccagatta
       61 ttctattgac caaagcaaaa aaacacatta tcttgttatt aaaaccccta aaacacccta
      121 tatattgggt tttttgctat aattatggta acagtaaaca aaagcttctc ttgactgtcg
      181 taaaaacagg agcaaatcat agtttcaatg aaactaggat cgccttttaa acaagttcgt
      241 aaaacatgtt ggattcactg aaacaatgaa ttgctaaaat atatttcgtg gaggaaaaat
      301 gtccgaaatt cttgagccgg taaaggatat accgctaaca aatcaactgg aattgactgc
      361 taatgtccca ggcatgctta aaacaataaa acgaaatggc aaggtagtca tttatgatga
      421 tactaaaatc aaaattgcta ttaccaaagc ctttattgcc gacgaaggtg gtacagcccc
      481 aacttctgat cgaattcacc agcaaattga agagctaacc agacaaatca cccaggtatt
      541 taaacgtcgt ttacctagtg gtggggctat tcatattgaa gagatccagg atcaagttga
      601 actcgccctt atgcgcagcg gacattataa agtagctcgc tcctatgttt tatatagaga
      661 agaacgcaga aaagcacgtg aatcagaatc gaaacaaaaa caaacaaaag acagtaagca
      721 tttattaata actatgccta atggagaagt acaaccatta gatatggaca gggtcaatac
      781 tatcgtcaaa gaatcatgtc gtgacctcag caacgtcaaa gcagaacctg ttattaaaga
      841 tgctttgcgc aatctttata accaagccaa atatgaagac gtccataaag ctttgattat
      901 gtcagcgcgt gcgttggttg aaaaagaacc taattatact tatgtcagcg cgcgtttgtt
      961 gctagacagc ttacgttctg aagcactgac taaactcaat atcaaatcag aagctacatt
     1021 tgatgaaatg gctcaaatct atccagttta tttcaaatct tacatagagc aaggtattgc
     1081 ccaaggtatg cttgacccca agatggctga cttcgacctg gacaagctta gccaagctct
     1141 tcttcccgaa cgagatatga agttcaccta tttaagttta caaactttat atgatcgtta
     1201 ttttattcat gagcgcgggg taagatatga gcttcctcaa gcttttttca tgcgagtagc
     1261 catgggtctt gctcttcgtg aacaacataa ggaagagaaa gccatagagt tttatcagtt
     1321 gctttcttcg tttgattata tgtcttccac tccaacctta tttaattccg gaacagtaag
     1381 gccgcagtta tccagttgct atttaacaac agtacccgat catttggatg gtatttatag
     1441 cgctataaaa gacaatgccc tcctctctaa atatgctgga ggactaggca atgactggac
     1501 tccagtacga gctatgggtg cccatatcaa agggactaat ggtaaatctc aaggcgtagt
     1561 tcctttcctg aatgttgccg atgctactgc tgtagcagta aatcaggggg ggaaacgcaa
     1621 gggagctgtc tgcgcgtacc tggaatgctg gcacagagat gtagaggaat ttctggagtt
     1681 aagaaaaaat accggtgatg atcgccgccg tactcacgac atgaatactg ccctttgggt
     1741 acctgatttg tttatgaagc gtgttcgtga agatggtaat tggactttat tttcacctga
     1801 tgaagtaccc gatttacatg atcaatatgg taaagcattt gaggctctct atgttgaata
     1861 tgaagaaaaa gctcgacaag gtattattag gaatacaaaa accatttcag ctgtcaaact
     1921 atggcgtaga atgctttcca tgctttttga aactggtcat ccatggctaa cattcaaaga
     1981 tccctgcaat ttacgatcac ctcaacaaca tgctggagta atacacagtt ctaatttatg
     2041 tacagagatc actctaaata cttctgaaga agaaattgct gtgtgcaatt taggcagtat
     2101 caatcttcca gcgcatatta aaaacggtaa actggatgag caaaaactca agcgcacaat
     2161 atcaacagct gtccgtatgt tggataatgt cattgatatt aattattatt ctgtacctca
     2221 agctcgtaac tccaacttgc aacatcggcc aattggtcta ggtatcatgg gctttcagga
     2281 tgcgctatac gaattaaaaa tggattatgc atccaaagaa gccattgaat ttgctgactc
     2341 ttccatggaa ctaatcagtt actatgctat cgaagcctct tgtgacctgg ctaaagagcg
     2401 gggcagttac tcaacctatg aggggtctct atggagcaaa ggcatattac ctatcgattc
     2461 aattaacctc ttgcaacaat cccgtaataa atatctggag caagacaggt cacaaaaact
     2521 ggattgggaa ccattacgag ttaaagttcg aacacaaggc atgcgtaact caaatgtaat
     2581 ggctattgca cctacagcca caatatccaa catctgcggt gtatcacaat ctattgaacc
     2641 aacttatcag aacctttacg tcaaatccaa cttgtccggc gaatttaccg tggtgaatcc
     2701 ttatttggta gccgatttaa aagctctgaa tctttgggat gaggtcatgg tcaatgatct
     2761 aaaatatttt aatggcagtg tacaacctat cagtcgaatt ccagatgagc tgaaaagacg
     2821 ttatgccact tcttttgaaa ttgaccccat gtggttggtt gaagccgcgt cacgccgtca
     2881 aaaatggata gatcaagccc agtccttgaa catctatatg gcgcaacctt ctggtaaaaa
     2941 actggatcag ctttatatgc atgcatggat tcgtggatta aaaaccactt actacttacg
     3001 tagtttaggt gcaagtaatg ctgaaaaatc cactgttacc gacggggcac ttaacgcagt
     3061 caaattaata gataccgaac caaaggcttg ttctattact gatccagact gcgaggcttg
     3121 ccaataatca ggagaaattt atgaactaca atgatactca agaaattatc gtaactcctc
     3181 ttatgggcgc gacagggctc gaaactttag aaatgggggc tgcacgcatt catgttgatg
     3241 ataagcaaat cattaattgc cgtgctgact taaatcaatt ggtgcctttc aaatacaaat
     3301 gggcttggga taaatacttg acagcctgtg ccaatcattg gatgcccaat gaaatcagta
     3361 tgagtgccga tgtcgcttta tggaaagatc cttatggtct tacagaagat gagcgtttaa
     3421 tcattaagcg taatttggga tttttctcaa ccgccgattc actcgttgcg aataatctgg
     3481 ttcttgctgt ttacagacat attactaatc ctgagtgtcg acaatatctt ctaaggcaag
     3541 cttttgaaga agcgttacac actcatgctt atcaatacat tatagagagt ttggggctgg
     3601 atgaagctga agtatttaat atgtatagag aaataccttc tgtatccagg aaagcagttt
     3661 gggcattacc atttactcaa agtttaggtg atgaaacctt ccatacaggc actccagaaa
     3721 atgatcaacg cctgctacgc gatttaattg cattttatgt cgtgtttgag ggtattttct
     3781 tttatgtcgg atttactcaa attctctcta tgggacgtcg taacaaaatg gtaggaacat
     3841 cagaacagtt ccaatatata ctgcgcgatg agtccatgca tatgaatttc ggtattgatg
     3901 tgattaatca aatcaaaatc gaaaatcctc acttatggac acctgaattt aagaatgaaa
     3961 tgatccaact catcagagaa ggtgttgagc tcgaatacca atacgccaga gacactatgc
     4021 ctcatggcat tctcggaatg aacgccgaga tgtttgaaga atatttgcac tttattgcaa
     4081 accgtcgctt aaatcaaata ggtctgccgg atcaataccc aggtgctgaa aacccatttc
     4141 cctggatgag tgaaatgctg gatctcaaaa aagagaaaaa cttttttgaa actcgtgtta
     4201 ttgaatatca ggctggtggt actttaagct gggatgatga ataaagttta atgatgttac
     4261 ctctagcaaa gtaagggagt tcatttagag aaatccctta ctttgtgaaa aatgtcatac
     4321 ccctgtcttt aacttaacca aacttaataa tattctacgt atttccatta tttttgaatt
     4381 aattttaaac aaatcaaccc ctaaatccaa atttatggtg acattttagt ttgatcaggt
     4441 aaaatcccaa gcgcatagaa ataatgctat gccagcaaaa taataacaac atcgctaaaa
     4501 gatataacac acccagaaaa atgcattcta ttaactgaaa atcaagatga ggtaagttat
     4561 gcagtctaaa atacacaaaa aagaagacaa tacaaattac agctttctat taaaatgtat
     4621 ggctgctgct acagcggcac ttgctacagc agcaatagta accgctgtgg taatcaaagc
     4681 cactgcgaca gcggcagtag gttttggtgt tgcagcaaca ggagccgctc tatcgccatt
     4741 attatttgta ggaggagcat tactggccgc tgtgggtatc tgctgccttc cattattgct
     4801 ctgctgtggc agcagccgta gtgtatatac cgtgtcaaac tcaccagcct acaggccttg
     4861 gagcagtgca tggtatacag gaggctataa tccaactttt cattcacatc ctgtttataa
     4921 caatggcggt acttaccaca ctcatcactc aggaaccgta catacccact ctaatccagt
     4981 acatacacat ggtaataatg ttcacgtcca tactactcca agtaatgttc atggacatag
     5041 ataattttat ctaaaccaag tgtattttat gtattgcttg acatagcaat ggttctaaac
     5101 aaaccattgc tatctaaaaa tcgctgtact ttactgtctc aaatgcggaa atagaataac
     5161 gtctctgatt gattgcgaat tagtaaataa catcaccaag cgatcgattc caatcccttc
     5221 accagctgta ggcggcaagc catattccaa tgcttcaata taatcactat caaaatgcat
     5281 tgcctctaaa tcacccgcat ccttttcttc aacttgcttt cgaaagcgct cagcttgatc
     5341 ctccgcatcg tttaattcag aaaatccatt tgcaatctca cgaccagcaa taaaaaactc
     5401 aaatcgatcg gtgacttccg gattggtatc agaacgtctc gctaaaggag atatttctgt
     5461 agggtattcc gtaataaaag tgggctgaaa taattggtgc tctactgtct cctcaaaaag
     5521 aataatttgt agtttaccca ggccatcagt ttctttataa ggcagaccta aatcattcag
     5581 aagtgttctg catccctcta ccgtttctaa ctgctgcgcc ttaatatcag gatgatattt
     5641 taatatagct tcttttacgc tcaaccgttc aaaaggttta ttgaaatcaa tgacctggcc
     5701 ttgatattct atttgccgag tagctaatac tttatcgcat aggtaatgaa acaattgttc
     5761 agtaaaattc atcaaatcat tgtaatcggc ataagcctgg taaaattcca gcatcgtgaa
     5821 ctctggatta tgacgagtag aaattccttc gttacggaaa tttctattaa tttcatatac
     5881 tctctcaaaa ccaccaacaa ccaagcgttt taagtacaat tcgggggcta tcctcaaata
     5941 catggtcata tccaatgtat tgtgatgagt tacgaaaggg cgagccaaag cacctccagg
     6001 aattggatgc atcattggtg tttctacttc cagaaaatga ttatcatcca taaattctct
     6061 gaaggctttg attaaatgtg accgtattag aaaagttttt cgactgtctt catttgcaat
     6121 caagtcaaca taacgttttc gatatttcaa ttcctgatca gccaaaccat ggaatttatc
     6181 aggaagtggc cttaaagatt tggttaacaa ctcaacatgt tctgcattaa tggtcagttc
     6241 accagtgttt gttttaaaca actcaccctg tacaccaaca atatctccta aatcccaatg
     6301 tttaaactgt tcataaatgt caggaagatc atttgaacga agataaactt gtatccgacc
     6361 tgatacatcc tgaatatgaa aaaaactggc tttacccata attcttctca gaacgattcg
     6421 gcctgcaaca gatactttca catgcttttg ttctaatgtt tgtttctcag tttcagagta
     6481 ttgcttcagc aaagcatcgg ctaaatgttc gcggcgaaaa gtgtttggaa aattaaaccc
     6541 acctgtgcgc aattccgcta atttttgctt gcgaatatga taaacttcac tttcatctaa
     6601 atgtacatgt tcttcactca tgcttgccct actcctgcct ttaaactggc ttcaataaat
     6661 tgatctaatg aaccatccaa tactgcctga gtattactgg tttcaacacc ggtgcgcaaa
     6721 tcttttatac gagattggtc taatacgtaa gaacgaattt gtgatcccca accaatgtca
     6781 gatttgctag cttccaatgc ttgctgttcc gcatttttct tttgcatttc caactcataa
     6841 agctttgccc tcaattgctt cattgcctgg tctttatttt tatgctggct tcgatcattc
     6901 tggcactgaa cgactatccc actagggata tgtgtaattc ttacggcaga atcagtacgg
     6961 ttaacatgct gcccaccagc cccggaagct ctgtaagtat caatccgcaa atcagctgga
     7021 ttaatttcaa tttcgatatc atcgtcaatt tcaggagaga cgaaaacagc tgcaaacgaa
     7081 gtgtgtctcc tgttgccaga gtcaaatggc gactttctca ctaaacgatg gacacctgtt
     7141 tcagtcctta accaaccaaa cgcatattca cctgtaaaat gaatagtcgc acttttaatt
     7201 cctgccactt caccaggaga gcattctatc aactcggtgg taaatccgtg atgttctccc
     7261 caacgtaaat acattcgtaa tagcatttca gcccaatctt gcgcttcagt gccgccagaa
     7321 cctgcttgaa tatcaagata agcattggag tggtccattt tgccagaaaa cattcttcta
     7381 aattccaaac tggcgacttg ttgctcaatt gattttaatt cctcaccaat gtcgttgatg
     7441 gtttgctcat catcttcttc tcgagccaat tcaaacagtt ccctctgatc actaattgat
     7501 tggcttaatt ggcttaatga gtggactata gtttccagct gagttctttc acgtcccaat
     7561 gcctgagcct gttccggatt gtcccacaca ttggatgatt ccaattcgcg aattacttct
     7621 tccaagcgct cacttttacc ttcgaagtca aagatacccc ctaagcgatt ctatgcgctc
     7681 attaagatcg attaaactta aattaatttg attcacttcc agcatcagtg tctcttttaa
     7741 aaataaatag gggattttac agcgaaagta agattctatc tatactaatt ctgatgtttt
     7801 aatgcggttc tatacgcttg aagattcatt agtaactctg cttcagcatt tgataaatga
     7861 caactactga caatttcttc acgagtgcca cccatttcca ggattctcaa ggcatgttga
     7921 taactgccat cgttatgtct tatgttttcc aattcttgca attgagtctc gatactaatt
     7981 aattgctgat taatttcatt aagttgtttc gcaaataata aatccgcatt gatcagcata
     8041 ggatgatctt ttactacttg agtagccaat ttttccaaag aatctatttt gttctgtaat
     8101 ttgttgatgt tttttcgttg atttaataaa taattaccca agagtaaaaa aattatcgca
     8161 tgca
//
LOCUS       DATNSN010000145         7984 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.145, whole genome shotgun sequence.
ACCESSION   DATNSN010000145 DATNSN010000000
VERSION     DATNSN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.145"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(80..838)
                     /locus_tag="VAH76_002530"
     CDS             complement(80..838)
                     /locus_tag="VAH76_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387127.1"
                     /translation="MNLVQRMNPFFWLVILAIYSTSSMALSVELIETSPKSPVILNQD
                     EALYVLIRYKSDQPLRFQAIGENLRQKIMDSARFNPSQAYPAGEGEAIAWVAYYNTTE
                     IDSITVTIYDANWRALQTKSIPVSAVWQNENGRNNQAAAPWVQRLNQQQQSSVFIQSQ
                     TPISSGDAQFVQLLFLLIPLYWFLQIIVIFNWTGRWKKLACFPLLFSIPLLLYTLYAL
                     CAGSNLWPLMMLFMTPFILVFLLIIIGYKKVYSR"
     gene            complement(972..1988)
                     /locus_tag="VAH76_002531"
     CDS             complement(972..1988)
                     /locus_tag="VAH76_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEO1387128.1"
                     /translation="MYSLDWPRAYGIPNSTATFKSLPEDFQVNELFEGQFSGEGEHIV
                     LKIEKRGLTTEEVVKSLARLINKPVKLISHAGLKDKQALTTQWLSVHAPGEIIDGIET
                     LEAPGWRILECTRHNKKLKPGFLSGNHFIITLRNVSDESDLIHRIEQIKFKGVPNYFG
                     EQRFGRDGGNLIKAEEILVQGRKVKDRFLKGMYFSAARSWLYNLILSRRVKESSWNLP
                     LLGDVIQLVGSNSIFVNDKSMDEQLLQRIGEKDVSPASPLPGRSKNLVKGTALQIIND
                     VYEEWSAWLDGLEKNGLEEAWRANILYAEQIEYRINQGTVELSFVLPAGAYATVVLRE
                     LVQY"
     gene            2281..3264
                     /locus_tag="VAH76_002532"
     CDS             2281..3264
                     /locus_tag="VAH76_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEO1387129.1"
                     /translation="MIVNSSFRPLWWLSNRHAQTLYPTLVKKRLKPRIDFHERLELPD
                     GDFIDLAWSVNELNKNTPLVILLHGLGGGINSIYVSGLMQAFADAGFRCVLMHFRGAS
                     EEPNRILRTYHSGDTADFAYFLEILAKREPATKKAVVGISLGGNVLLKWLGETASSLW
                     VDAAVAVSVPFQLNSVADRMNQGFSRLYQAYLLSRLRRIFLKKLNHLNNSPFSKNDVL
                     SLKTFWDFDEQITAPINGFKSAHEYYQKASSRQYLKNISTPTLIIHAQDDPFMTPDVI
                     PTLEELSSQTTLELSEYGGHVGFIAGTGKSSSQPVYWLEQRIPEFLLDYLS"
     gene            complement(3289..3684)
                     /locus_tag="VAH76_002533"
     CDS             complement(3289..3684)
                     /locus_tag="VAH76_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387130.1"
                     /translation="MQNEKEVKELIQSDKKKRVTWNDNQTKGQIEEKFLKVGTASTPY
                     KNKLDTRIAQLMEEGYSEIEATSITGKSVTPRGKVIYNNPVFYKSKESIDIEKANRYN
                     FFSIRNIGLGVVAIAAAAATVGLVVSKNS"
     gene            complement(3919..6126)
                     /locus_tag="VAH76_002534"
     CDS             complement(3919..6126)
                     /locus_tag="VAH76_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO1387131.1"
                     /translation="MAHNHNDGHSHVHNEHVCCHKKSNTHKPQITSEGTIFYTCPMHP
                     EVRQATPGICPLCGMALEPEAISIDEATNPEYIDMKHRFWLALILTLPVVVLEMAGHG
                     FQHFVSTGISNWIQFLFATPVVLWCGWPFFLRGWQSLKARQLNMFTLIAMGIGVAWVY
                     SVVAVFFPGLFPVAFRSQEGVVSVYFEAAAVITTLVLLGQVLELKAREQTGSAIRALL
                     KLAPENAHRIKEGGSEEEITLDQVEVGDLLRVRPGEKIPVDGEIQEGHSFVDESMVTG
                     EPMPVAKEIGDKVIGATINQTGSFIMKALHVGADTMLARIVQMVSDAQRSRAPIQRVA
                     DTVSAWFVPTVILIAVLSFIIWGLLGPQPSLGYGLIAAVSVLIIACPCALGLATPMSI
                     MVGVGKGAQNGVLIKNAEALERMEKVNTLIVDKTGTLTEGHPKLTHIETSEDWDERQA
                     LLLAASLEYHSEHPLASAIVKAAQEMQITFSSVQNFEAPTGRGVVGRVNSHHVAIGNA
                     KLMQEYGSDDAALFEKADKFRAKGSSVMFMAVDGKTVAFLVVEDPIKSTTAETIHALQ
                     KKDIEIYMLTGDSKKTAEAVAATLGIKNVIAEIMPEDKGRIVSELKENGLIVAMAGDG
                     VNDAPALAKADVGIAMGTGTDVAIESAGVTLLHGDLRGIAKARYLSEYTMNNIRQNLF
                     FAFIYNMLGVPIAAGIFYPLTGLLLNPVIAAAAMALSSVSVIVNALRLRRVTL"
     gene            6335..7195
                     /locus_tag="VAH76_002535"
     CDS             6335..7195
                     /locus_tag="VAH76_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387132.1"
                     /translation="MKKILITLIGFLFMNTYHHLLYADNSHHHTVKDLSTSSSKLENT
                     PTIQLEVQKIIDKKDKKLVFVKLTDIKSNKPITFNDLTEIHTKKFHLLIIDNHLIDYS
                     HVHPAETTTPGIYQFEWQPNFKNATYRAWADLLPTNTNKQEYATADLTSPQTVTRTTN
                     PIHHRTVLESTVNGYQFKLTFNKMPLLAGKPAMGEIDVTDSKGHPVRSLEPIMGAYAH
                     IVGFNEDFKTVIHVHPMGKEPANSTERGGPQLQFHIEPNKAGFIKMFAQVQIDGKVLY
                     APFAFTVKNA"
     gene            complement(7288..>7984)
                     /locus_tag="VAH76_002536"
     CDS             complement(7288..>7984)
                     /locus_tag="VAH76_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HEO1387133.1"
                     /translation="SAPASVLLMLASHQDILKITPKKIFSVAEVLEPKEEEILTKAFG
                     CTISQIYQCTEGFLAISERKTNRLLMNEEFLIIEKEWLDEERFVPIITDLYRSTQPII
                     RYRLDDVLVTERSNGVFTELKNIEGRLGDVCYGKRGQEIVPIFADVIRQHMISSGVSF
                     EDYLICQQTLTQFTIQISPEISNKNQFIEHLNQLFIYKNCELPQWNWELYDKNQPGVK
                     RRRVRSLYCPKTE"
BASE COUNT         2474 a         1681 c         1366 g         2463 t
ORIGIN      
        1 tgataaaaga ctcagaaaaa ttcaaatttg ataatagatt aaacttttat actcaagata
       61 agcattggat gcgccttaat tatctggaat agactttttt atatccaata atgatcaata
      121 aaaataccaa aataaatggt gtcataaata acatcatcaa aggccataaa ttagaacctg
      181 cacataacgc ataaagcgta tataacaaca aaggtatgct aaataataaa gggaaacacg
      241 ccaatttttt ccatctcccc gtccaattaa aaataacaat aatttgcaaa aaccaataca
      301 aaggaattag taaaaaaagg agttgaacaa actgggcatc accagaagaa atgggagttt
      361 gtgattgaat aaacacactg gattgctgtt gttgattcag tctttgaacc cagggagctg
      421 ctgcttgatt gtttctacca ttctcatttt gccagactgc agacacagga attgatttgg
      481 tttgcaaagc cctccaattt gcatcataga ttgtgacagt aatggaatca atttctgtgg
      541 tattatagta agctacccaa gcaatagcct ctccttctcc ggctgggtat gcttgtgatg
      601 ggttaaacct ggcgctatcc attatttttt gccgcagatt ttccccaata gcctggaatc
      661 gcaaaggttg gtcactctta tatcgaataa gcacatacaa agcctcatcc tggtttaata
      721 taacaggaga tttaggactg gtttcgataa gctccacact caatgccatg gatgaagttg
      781 aataaatagc caggatcact agccaaaaaa aagggttcat cctttgaacc agattcatga
      841 atgtgtccct caattaaata aacatttctc tttattatgg cataacatca aacatagtga
      901 ttaattcatc aattcaatta aaaaataatt actcaacatc gaataaagtg ctattcaagt
      961 gcaaacaaag cttaatattg aaccagttca cgtaatacca ctgtcgcgta agcgccagcc
     1021 ggcaagacaa acgaaagctc aactgtacct tggtttatcc tatattcaat ctgctctgca
     1081 taaaggatgt ttgcacgcca ggcttcttcc aaaccatttt tttctaaccc atcaagccaa
     1141 gcagaccatt cctcataaac gtcgtttatt atttgcaaag ctgtcccttt tacaagattc
     1201 ttactcctac caggcagagg gcttgctgga gaaacatctt tttcaccgat tcgttgcaac
     1261 aattgctcgt ccatgctttt atcattaaca aatatactgt ttgatccaac aagctgtatg
     1321 acatccccca ataagggcaa attccatgat gattccttta cacggcgaga caggatcaaa
     1381 ttatatagcc atgatcgggc tgcagaaaaa tacattccct ttaaaaatct atcctttact
     1441 ttgcgcccct gtactagtat ttcctctgct ttaattaaat ttccaccatc tcgaccaaaa
     1501 cgctgctcac cgaaataatt tggaacacct ttaaatttaa tttgttcaat acgatgtatt
     1561 agatcactct catcagagac atttcttaaa gtgattataa aatgattgcc cgataaaaaa
     1621 cctggtttga gttttttgtt atgacgcgta cattccagta ttctccaacc tggcgcttct
     1681 agtgtttcta tgccatcgat tatctcccca ggtgcatgaa cactgagcca ttgagttgtt
     1741 aatgcctgtt tatcctttaa tccagcatga cttataagtt ttacgggttt attaattaac
     1801 ctggctaatg atttaacaac ttcttcggta gttaatcctc ttttttcaat tttaagaact
     1861 atgtgctctc cttcaccact aaattgtcct tcaaacaatt cattcacttg aaaatcttcc
     1921 ggcaacgact taaaagtagc tgtagagttt ggtatgccat aggctcgtgg ccaatccaaa
     1981 gagtacatca agatctcctt caacaaaaaa gggttctaaa ctaaatccac aaattcacta
     2041 taagctcaca gagatacaga acttattagt tgagataatg catctcttcg caaaatacct
     2101 atcttaacta tcgtttgtca tttgatatag ggactataaa acattatgat gcaagattac
     2161 caacaaaaat tagtttaaat tcttgattga ctataattta aatagatatt ttcttttgca
     2221 atttctgatc atggtctgcg ttatccagtt agaattaaat tttttatttt caggttatat
     2281 atgattgtaa acagttcatt taggcccctg tggtggttaa gtaacaggca tgcgcaaacc
     2341 ttgtatccta ctttagtaaa aaagcggtta aaaccgcgaa ttgattttca tgagcgactg
     2401 gaattacctg atggtgattt tattgatctg gcctggtctg ttaatgaatt aaataaaaat
     2461 acacccttgg tcatattact gcatggtttg ggtggaggaa ttaactccat ctatgtcagt
     2521 ggattaatgc aagcattcgc cgatgccgga tttcgttgtg ttttaatgca ttttcgcgga
     2581 gccagtgaag aacccaaccg tattttacgt acctatcatt ccggtgacac ggctgatttt
     2641 gcctattttc ttgaaatttt ggcaaagaga gaacctgcga caaaaaaagc ggttgtcggc
     2701 atatccttgg gaggcaacgt tctactgaaa tggttgggcg aaacagcatc atctttatgg
     2761 gtagatgcag ctgttgctgt ttccgtacca ttccaattaa attcggtggc tgacagaatg
     2821 aatcagggtt tttctcgcct gtatcaagct tacttattaa gtcgactgcg ccgtattttt
     2881 ctaaaaaaat taaatcactt aaataattcg ccattttcaa aaaatgatgt actttcctta
     2941 aaaactttct gggattttga tgagcaaatt acagcaccaa tcaatggttt taaaagtgca
     3001 catgaatatt accaaaaagc aagctccaga caatatctta agaatatcag cacgccaact
     3061 ttgattattc atgcgcagga cgatcctttc atgacaccag atgttatccc aacattggaa
     3121 gaactatctt ctcaaaccac tttggaatta agcgagtatg gcgggcatgt aggctttatt
     3181 gccgggactg gaaaaagctc aagccaacct gtgtattggc ttgaacagcg cattcctgaa
     3241 tttttgctag attatttatc ttaaaaaatc aatggaaacg taatacgttt agctattttt
     3301 tgatacaacc agtccgactg ttgcagcagc agctgctata gccactacac ctaatccaat
     3361 atttcttatt gagaaaaagt tataacgatt cgctttttct atatcgatgc tttccttaga
     3421 tttataaaaa acaggattat tgtaaataac cttacccctc ggtgttaccg attttcctgt
     3481 tattgatgtt gcttcaattt ctgaataacc ctcctccatc aattgggcaa tcctggtatc
     3541 caatttgttt ttatatgggg ttgaggcggt gccgacttta agaaattttt cctcgatctg
     3601 ccctttagtc tgattatcat tccaagtaac tctttttttc ttatctgatt gaatgagttc
     3661 tttcacttct ttctcatttt gcatacaaac ctctctataa ttaatcaaaa tttaaacaat
     3721 attagcaata aatacttaag aaaatatgaa ctcaaaaggt aatttaaaaa ttgcatctat
     3781 ttggaaaccc agatgaaatc gagttgatcg attaaattcc atatcagatt gtaacttgtt
     3841 gtttattaaa caatgtattt ctgattagta tacaatcagg agtcattaca gaatgattta
     3901 atacacttta ctatcgattc acagtgtaac tcgtcgcagc ctcagagcat taacgataac
     3961 tgatacagaa gaaagtgcca tagctgctgc agcaatcact ggatttaaga gcagacctgt
     4021 taatggatag aaaatacctg cggcaatggg gacacccaac atattataaa taaaagcgaa
     4081 gaagagattt tggcgtatgt tattcattgt gtattctgat aaatatcgag ctttggcaat
     4141 accacgtaaa tctccatgta gcaaggtaac gccagcgctc tcaatagcaa catcagtgcc
     4201 tgtacccata gcaataccaa catctgcttt tgccaatgcg ggagcatcat ttactccatc
     4261 gcctgccatg gcgacaatta agccattttc ttttaattcg ctgacaatac gacccttatc
     4321 ttcaggcatt atttctgcta tgacgttttt tatacccaga gtagctgcta cagcttctgc
     4381 ggtttttttg ctatccccag tcagcatgta aatctcaata tcctttttct gaagtgcatg
     4441 aatggtttct gcggtagtag atttaatagg atcttctaca actaaaaacg ctactgtttt
     4501 accatcgact gccataaaca taacagaaga accttttgca cgaaatttat cagctttttc
     4561 aaaaagagca gcatcgtcgc ttccatattc ttgcattaat tttgcattac cgatagctac
     4621 atgatgacta tttactctac ctactacacc cctcccggtt ggcgcttcaa aattttgaac
     4681 tgagctgaaa gttatttgca tttcttgtgc tgctttcaca atggcgctag ccaggggatg
     4741 ttcactgtga tattccagag aagcagctaa tagcaatgct tgtctctcat cccaatcttc
     4801 gctggtttca atatgggtaa gtttcgggtg tccttccgta agtgtgcccg ttttatctac
     4861 gatgagcgta tttacctttt ccattcgctc aagcgcttca gcgtttttga ttaatactcc
     4921 attttgtgcg cctttcccca cacctaccat gatagacatt ggtgttgcca aacccaacgc
     4981 acatgggcaa gcaataatga gaactgatac agcagcaatt aagccataac caagagatgg
     5041 ctggggacct aatagccccc atataataaa agacaatacc gctatcaaaa tcactgtggg
     5101 cacaaaccaa gccgagacag tatctgctac acgctgaatt ggagcacggc ttcgttgagc
     5161 gtcactcacc atttgaacaa tacgtgccag catggtatct gcaccaacat gcaatgcttt
     5221 cataatgaag ctacctgttt ggttaattgt agcgccaata actttgtctc caatttcttt
     5281 agcaacaggc ataggttcac ccgttaccat ggattcatct acaaaactat gtccttcttg
     5341 tatttcaccg tccacaggta ttttttcacc tggccgtaca cgcagcaaat caccgacttc
     5401 tacttgatcg agcgttattt cctcttcact accgccttcc ttaatgcgat gcgcattttc
     5461 tggtgctaat ttcagtaaag cacgaatagc actacctgtt tgctctcgtg cctttaattc
     5521 aagcacttgc cctagcagta caagagtggt tatcacagca gctgcttcaa aatatacaga
     5581 gactactcct tcctggcttc gaaacgcgac ggggaataat ccgggaaaaa aaaccgccac
     5641 cacactataa acccacgcga caccaatacc catagcaatc aaagtaaaca tattcaattg
     5701 acgtgctttg agtgactgcc aaccacgaag gaaaaaaggc caaccgcacc atagcaccac
     5761 gggtgttgcg aatagaaact gaatccaatt tgaaatacct gtcgaaacaa aatgctggaa
     5821 tccatgccca gccatttcta agactactac aggtaaagtc aagatcaacg ccagccaaaa
     5881 tcggtgtttc atatcgatgt attccggatt tgttgcctca tcaatggaaa tagcttctgg
     5941 ttctaatgcc attccacaaa gtgggcaaat ccccggggta gcctggcgaa cttcaggatg
     6001 catagggcaa gtatagaaaa ttgtcccttc tgaagtaatt tgtggtttat gtgtattact
     6061 ttttttgtgg caacaaacgt gctcattatg aacatgggaa tgaccgtcat tgtggttgtg
     6121 tgccattatt cacctatccg tagttttatt ctgcctggaa tttataacca tttaaattag
     6181 ctcataatac cctgttatgc tggtttatat ctacaacgtt gtaataaata atgcatttct
     6241 taccctgtaa gaatcaacta ctttcaatta taattcatcg ttgacagaag aaaaatttag
     6301 cttggcaata aaaaattacg gagatcattg tattgtgaaa aaaattctca tcacgctaat
     6361 tggcttttta ttcatgaaca cctatcatca tttgctctat gccgataatt cccatcatca
     6421 cacggtaaaa gatctttcaa catcttcctc taaattggag aatacaccca caatacaatt
     6481 ggaagtccag aagataattg ataaaaagga taagaaactg gtatttgtca aactcactga
     6541 tataaaatcg aataaaccaa ttactttcaa cgatcttact gagatacata caaaaaaatt
     6601 tcatttactg atcattgata atcatctcat tgactatagt catgttcatc ccgcagaaac
     6661 tactactcca gggatttatc aatttgaatg gcaacccaat ttcaagaatg ccacctatcg
     6721 ggcatgggct gatctattac caaccaatac caataaacag gaatatgcca ctgctgatct
     6781 aacatctccc cagacagtaa cgagaacaac gaatcctata catcatcgaa cagtattaga
     6841 gagtacagta aatggatatc aattcaaatt aaccttcaat aaaatgcctc tactagcagg
     6901 taaacctgcg atgggtgaaa tcgatgtgac tgattctaaa gggcatccag tccgttcact
     6961 agaaccaatt atgggggcct atgcacacat tgtcggcttt aatgaagatt ttaaaaccgt
     7021 tatccatgtc caccctatgg gcaaagagcc tgcaaatagc actgaacgtg gagggcctca
     7081 attacagttc catattgagc ctaataaagc aggtttcatt aaaatgtttg cccaggtgca
     7141 aattgatggg aaagtactct acgcgccatt tgcattcaca gtaaaaaatg cttaacgact
     7201 aaattaagaa cctgtctttt acagacgcaa atattattgt aagctcctag cttgtgttgt
     7261 gcagagaaac cagttctttg tacccgctca ttctgtttta gggcagtata gtgagcggac
     7321 ccgtctgcgt ttcacaccag gttgattttt atcgtagagc tcccagttcc attggggtaa
     7381 ttcgcaattt ttatagataa aaagttgatt caagtgttca atgaattgat ttttattgct
     7441 tatttcgggt gatatctgaa ttgtaaattg ggttaatgtc tgctggcaaa ttaaataatc
     7501 ttcaaaactt acaccactgc taatcatatg ctgtcgaata acatctgcaa aaatgggaac
     7561 tatttcttgc cctcttttcc cataacaaac atcacctaaa cggccttcaa tattctttaa
     7621 ctcagtaaat acaccattcg atctttcagt caccaaaaca tcatcaagac ggtaacgaat
     7681 aattggctga gtagatctgt acaagtcagt aataattggc acgaatcgct cttcatccaa
     7741 ccattccttt tcaataataa gaaactcttc attcatcaat aagcgattgg ttttacgttc
     7801 actaatagcc agaaaacctt ctgtacattg ataaatttgt gatatggtac aaccaaatgc
     7861 tttggttaaa atctcttctt cctttggttc gagtacttcg gccacagaaa atattttttt
     7921 tggtgtaatt tttaaaatat cttgatggct agcaagcatt aaaagcacac tggctggagc
     7981 tgaa
//
LOCUS       DATNSN010000146         7978 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.146, whole genome shotgun sequence.
ACCESSION   DATNSN010000146 DATNSN010000000
VERSION     DATNSN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.146"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            37..873
                     /locus_tag="VAH76_002537"
     CDS             37..873
                     /locus_tag="VAH76_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEO1387134.1"
                     /translation="MLINRAGQTLYGGAPAMGSAYPTVEKVNAVPGGSGDSNNNPSAS
                     EQVMKGMIIRNANITLEVNDPAKVIDEIMQMAEREGGYAVNSNLRQDSFYVGGNIAQI
                     SIRIPSNRLNNVLQNLKSLAVKVIEESITSEDITRQYVDLESELKNYKVAKDQLNKIM
                     QGAKNTSDVLAVYQQLSETQRKIDIIEGQIKYYKESVAYSLITIYLQINPAIKNAQNS
                     TWQTSEVVRKSYQALIEHLRQFTYGFIEFVILFVPMILLWGIIALLVFWIGRAVYSRF
                     KQ"
     gene            complement(959..1156)
                     /locus_tag="VAH76_002538"
     CDS             complement(959..1156)
                     /locus_tag="VAH76_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387135.1"
                     /translation="MLKTLGGATRTAALDTARVTATTTVVLGGMYLWDRVAKNYGLPN
                     KENSKPISLDLGNDNYMGPKV"
     gene            complement(1405..2379)
                     /locus_tag="VAH76_002539"
     CDS             complement(1405..2379)
                     /locus_tag="VAH76_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1137 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387136.1"
                     /translation="MIQRGFTMQERREKQGNKSPYLLTPYEMANLVFKTGAISFTVSA
                     GTQPFQYLLNKLQFSQSGTPSGLSGGLFRGMYRGFLPYAIAGQKRGAVAVTHKQTNKA
                     TEEEEFEAPVRQRWLGTVLFSQADLLVSNGLSGKARLQNVGVINAENFKWSFSNFWKL
                     TTVNWGSRSFAGGVNFALIGFAGDYVSSFYKFDKDLYNKIAGGATSGIIATLFTTIPN
                     AYADNKLLQTKVAENNRLVTVSPYTMFGQMKSHVKAVGLKEAFLTFFKVGYLQQVAVR
                     APQAAITFALIFGMDEYMGPQPLKKVWPGRVEIEELESETPSPSPTKK"
     gene            complement(2639..3661)
                     /locus_tag="VAH76_002540"
     CDS             complement(2639..3661)
                     /locus_tag="VAH76_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO1387137.1"
                     /translation="MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKVIHQFEQESGI
                     KVNFSTYDSNETMYAKLRASQKSIYDVILPSAYFVERMQKQGMLQLLDHKQLSNLHNL
                     DEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDS
                     REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGS
                     AWNGDAFKAHTENKAIEFIYPQDGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSA
                     QIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRDIGEETLALYNQYWE
                     QLKLAF"
     gene            complement(3658..4425)
                     /locus_tag="VAH76_002541"
     CDS             complement(3658..4425)
                     /locus_tag="VAH76_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO1387138.1"
                     /translation="MKTMTQRLFLSLVYAFLYIPIAILVLYSVNDAKFSLQWHGFSLR
                     WYKELFHDRGLWSAVFNSITLGLTSSLIATTMGLLASINLFLFRTRHRQILYTLLLLL
                     IIIPDIVLGVALLIFFNITQVPLGFLSLLVAHITFSIPFVILTINSRINTLNPNIYFG
                     ALDLGASRYFALTRVLLPLLWPAVLSAFLLSFTLSFDDVIISYFVAGPDFTILPLTIY
                     SLVRAGVTPELNALCSITFGLSMILVIISHRLSGKLI"
     gene            complement(4422..5246)
                     /locus_tag="VAH76_002542"
     CDS             complement(4422..5246)
                     /locus_tag="VAH76_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO1387139.1"
                     /translation="MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPL
                     TLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIP
                     FWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
                     LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYI
                     PNVLGGARSILLGNLIQNQFLVLSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR"
     gene            complement(5236..6330)
                     /gene="potA"
                     /locus_tag="VAH76_002543"
     CDS             complement(5236..6330)
                     /gene="potA"
                     /locus_tag="VAH76_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HEO1387140.1"
                     /translation="MTTPLIEIRQIYKSYGNTPILNNVSLNVNHGEFLTLLGPSGCGK
                     TTLLRLISGFEQPTQGEIFINGQCVNQLPPQKRDVHTVFQSYALFPHLSVFENVAFAL
                     RCKKTPNQEIKERVFDALKLVQLESLAERNVKQLSGGQQQRVAIARAIINRPQVLLLD
                     EPLSSLDYRLRKAMQSELKQLQKTLNMTFIFVTHDQEEALSMSDRIVVFNHGHIEQIG
                     TPKAVYETPANLHVAMFIGEANIFDIQIHTVKDQDIVTNIEGIQLFCKNTGNYQVNDR
                     LHLIVRPEDIRVWSLSEVEKTEGMLPGRIVDIIYKGSTVDLKVELSSGKIINASEFFD
                     EDDDKLEYTLHETVWVQWLPGWEVLLPYEG"
     gene            complement(6499..6792)
                     /locus_tag="VAH76_002544"
     CDS             complement(6499..6792)
                     /locus_tag="VAH76_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO1387141.1"
                     /translation="MEEKQYVVYVLASKAYGTLYTGITSNLVQRIYQHKKGLTEGFTK
                     RYNVHRLVYYEIHTDVYEAITREKRIKKWNRQWKINLIEQKNPQWLDLSIGLC"
     gene            complement(6992..7876)
                     /locus_tag="VAH76_002545"
     CDS             complement(6992..7876)
                     /locus_tag="VAH76_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO1387142.1"
                     /translation="MTKTDKKIVVVTGCSKTSGVGYNLVCEMLKRGHQVIATVRNLET
                     SQINKANLPNSSNLDVKILDLCDENSIDRFIKEVLSQYQYIDALVNNAANVAIGPVES
                     TTTQDVLTTFQTKVFGPLSLIRGFIPVMRERKKGLLTTTSTIFTSVPVSIPGVAIYFS
                     ALAAFERLQESLAIELAPWNIKVINFQPGPIATELTRFEGSRADIVEKYYKNFTEQAY
                     QWFHKNTVFQNATEVAKVFAEVIDMENPDLCYQSNLFGKQFVAKNKKDITGREGMNEL
                     LEHFKNLSHDNPDDWKIQ"
BASE COUNT         2521 a         1465 c         1549 g         2443 t
ORIGIN      
        1 attggagttt acgctgtcct tctatttgcg tacaacatgc tgatcaatcg agccggacaa
       61 acattatatg ggggcgctcc agcaatgggg agtgcctatc ccactgttga aaaagtcaat
      121 gcggtaccag gtggttctgg tgatagcaac aataatcctt ctgcttcaga gcaagtcatg
      181 aaaggaatga taattcgcaa tgcgaatatt actctggagg tcaatgatcc ggctaaagtt
      241 attgatgaaa ttatgcagat ggccgagcga gagggaggat atgcggttaa ctctaattta
      301 agacaggata gtttctatgt tggtggcaat atcgctcaaa tcagcatcag aataccaagt
      361 aatcgtttaa acaatgtatt gcaaaattta aaatctttag ctgttaaagt gattgaggaa
      421 tcaattacga gtgaagacat cacccggcaa tatgttgatc ttgaaagtga gctgaaaaat
      481 tacaaggttg ctaaagatca attaaataag attatgcaag gcgccaaaaa cacttcagat
      541 gtgcttgctg tttatcaaca gctgagtgaa acgcagagaa aaattgatat tatagaaggg
      601 cagataaagt actataaaga atctgttgcc tattctctca ttacaattta cttgcagatc
      661 aatccggcaa taaaaaatgc acaaaattca acatggcaaa catcagaagt tgttagaaaa
      721 tcttaccagg cattaataga gcacttaaga cagtttacct acggattcat tgaattcgtt
      781 atcttatttg tcccaatgat tttattgtgg gggattatag ctttattagt attttggata
      841 ggtagggctg tttattcccg gtttaaacaa taggacttac tgtgtatctc agattttggt
      901 ttaagagaga gctaaatact cccaacagga tagttgggag cggaaataat cttttagttt
      961 aaactttagg acccatataa ttgtcattac ccagatcaag gcttattggt ttggaatttt
     1021 ccttgttagg tagcccataa tttttagcta ctctgtccca taaatacatg ccgcctagaa
     1081 ccactgttgt tgttgctgtt actctcgcgg tatcaagtgc cgctgtcctg gttgctccgc
     1141 ctaaggtctt taacatacat ttcccaaaga atgataacat atttaccgtc ctgattagtt
     1201 aataataatc ctgctaatta ttcatcattc attctaaatg aatttctgcc taatgcaata
     1261 catttccctg tttatatgat taaaataaaa tcaaccatgt cttttttaac gttatgtgga
     1321 ttaaataagt acaatttata ccgtaaaacg tattgttgtt tgttaaggca caatgatgaa
     1381 aaataacatc cttgttactc ttaattattt cttggtaggg gatggggatg gagtctctga
     1441 ctcaagctct tcgatttcga ctcgtccagg ccaaaccttt ttcaaaggct gaggacccat
     1501 atattcatcc ataccaaaaa tgagggcaaa agtgatggct gcttgaggag ctcttaccgc
     1561 tacttgctgc aaatagccaa ctttgaaaaa ggtcaggaag gcttctttaa gaccaaccgc
     1621 ttttacatgt gatttcattt gaccaaacat ggtatagggt gacacagtaa ctaaacggtt
     1681 attttcagcc actttggttt gcaacaattt attatcagca taggcgttag gaatggttgt
     1741 aaacaatgtc gcaatgatac cggaagtagc gccacctgct atcttgttat acaagtcttt
     1801 atcaaattta taaaatgaac tgacataatc ccctgcaaat cctatcaatg cgaaattgac
     1861 tccaccggca aaagaacgag aaccccagtt aacagtagtc aatttccaaa agttggaaaa
     1921 ggaccatttg aagttttcag cattaataac cccaacattt tgcaacctgg ccttgccact
     1981 taaaccgtta ctgactaaaa gatcagcttg agagaagagt actgttccca accaacgttg
     2041 acgaactgga gcttcaaatt cttcttcttc tgttgctttg ttggtttgct tatgggttac
     2101 tgctacagca ccgcgttttt ggcctgcaat agcataagga aggaatccac ggtacatgcc
     2161 tcggaaaagt cctccgctca atccagatgg agttccagat tgtgaaaatt gcaatttgtt
     2221 tagcaagtat tgaaatggtt gagttcctgc agaaacagtg aaagaaatag ctcctgtttt
     2281 aaaaactaaa ttggccattt catagggtgt caacagatag ggtgatttat taccctgttt
     2341 ctctctacgt tcttgcattg taaatcctct ttgaatcact taaatcaatg tgctgtaatg
     2401 ataacaattt attattaagc aaatattaac tggtgtgaaa aaaaaatatt aagtatgaaa
     2461 ataatatgta aaaagtgaga caaataagac cataaaatag attgaattta tacaaattaa
     2521 gcataattat tttctttttg tatataaaat attcaaaaaa tcttgctttc tgaattttaa
     2581 ttcgttgagt taatgaagta cttcatctgg atatttagcg ctacttcaca acagaaaact
     2641 aaaaagcaag ttttaattgt tcccaatatt gattataaag cgctagtgtt tcttcgccga
     2701 tgtcgcgttg gaaataaccc ttctttagtg ttttgtctgg cggataaacc acagggttat
     2761 tttttatagg taatggcaat aaagctcttc ctgcggcatt ggtaatcgca tgtccttcct
     2821 ttagtgcaat ttgagcgctt gtttccggtt tcagaataaa attaatgaat tgatacgcct
     2881 ctttcaggtg tggggggttt ttagggatag ccaaacaatc cacccaaatc acaaaaccat
     2941 cttgaggata aataaactcg atggctttgt tttctgtatg tgctttgaag gcatcgccat
     3001 tccatgctga tccaataaaa gcatcctcat ctatcatgat ggcttgaata ctatcgctgg
     3061 caaataactt aatattaggt accaattgca ataagtgttc ataggctgct tttatgtggt
     3121 ttggattggt gtcattggga tcaaacccta aactcatcag agctatggca aatatttcgc
     3181 gtgaatcatc aagtaacatt agctgattgc gccacttttt atcccataag ccttgccagc
     3241 tttttggcgg gttcgatacg gaattatgat taaagaaaat cccggttgct ccccagatga
     3301 tgggtatact ataacggtta cccgggtcat aatcattttt aacaaattgt tcatccagat
     3361 tatgaagatt cgaaagctgt ttatgatcga gtaattgcaa catgccttgt ttttgcatgc
     3421 gctctacaaa ataagcggaa gggaggatga catcataaat gctcttttga ctggctctta
     3481 gcttggcata catggtttca ttgctatcat aagtcgaaaa attgacttta attcctgact
     3541 cttgttcgaa ttgatgaatt acttttttag ggatttcacc tccccaggca tagacattga
     3601 ctactggatt ggagaatgcc ttaaatgtaa gaagagcgag cagtaatgaa aaaattttca
     3661 tatcaattta cctgacaagc gatgagagat aataacgaga atcatcgata aaccgaacgt
     3721 aattgaacac aatgcattga gttcaggcgt gacaccagca cggactaaag agtagatggt
     3781 caaaggcagg atagtaaaat cagggcctgc cacaaaatag ctgataatga cgtcatcaaa
     3841 tgacaaagtg aaacttaata aaaaagcgct taaaaccgca ggccacaata aaggaagcaa
     3901 gactctcgtt aaagcaaaat aacgactggc gccaagatca agggcgccaa aataaatgtt
     3961 gggatttaaa gtgtttattc gtgaattaat ggtcaagata acaaaaggaa tggagaatgt
     4021 aatatgggcg actaaaaggc tcaagaagcc aagcggtact tgggtaatat taaagaaaat
     4081 taataaggca acacccagga ctatgtcagg aataataata agtaatagta ataaggtata
     4141 cagaatttgg cggtgtcttg tacgaaataa aaataagttg attgaggcca atagccccat
     4201 tgttgtagcg ataagagagg atgttaggcc cagggtgatg gaattgaaaa cagcagacca
     4261 caagcctcga tcatgaaata gttctttgta ccatcgcaaa gaaaacccat gccattgtaa
     4321 tgaaaatttg gcatcattta ctgaatagag caccagtatt gcaatgggga tataaagaaa
     4381 agcataaact aaagaaagaa acaggcgctg tgtcattgtt ttcatcgaat gacctcattg
     4441 ttctgtcgcc gaaaaatgaa cattaaaata agcagaacgg aagttaatag cacactggta
     4501 gcggctccct gaggccaatc gctcaggaca aggaattgat tttggattaa atttcccaat
     4561 aaaatggaac gagcgccacc caatacatta ggaatgtaaa ataaagtcat tgctggtaac
     4621 aagaccagca aacaacctga taagattcca ggggctgtgt taggcaaaaa tactcgtgta
     4681 aatatggacc acttgtttgc ccctaaatct ttagctgcct caattaacct gaaatcgaac
     4741 cgttccatat tagtaaaaat cggtaggacc atgaaaggga aaagattgta aaccaagcca
     4801 ataacgaccg caaaattggt ataaagtagt gacaagggaa catcaatcaa gtgtaatttc
     4861 agcaataggg catttatcac gcctttgtat ttaaggatag caatcaaagc ataagttctt
     4921 atcaatgagc ttgtccagaa aggtatgata atcaacaaca ataaaataga ttggtgtttt
     4981 gatttaatca ttagatagct aaaggggtat gcgatgagca gacaaagcag ggtggtgaca
     5041 caggctatca gtaaggatcg gaaaaggatt tttgcaaaaa caggtgtaaa caagtcaaaa
     5101 taattttcca atgtcaatgg caaggtaaca agatgaatac tgtcttgtga taaaaagctg
     5161 gcaaccagca ccatcaacaa gggaatgaag ccaaaaataa ttaaccaaaa atacaataaa
     5221 tacagtgcgg atgatttagc cttcataagg taataaaacc tcccagccag gcagccattg
     5281 tacccaaacg gtttcatgga gagtgtattc cagcttatca tcgtcttcat caaaaaactc
     5341 cgaagcattg atgattttgc ctgatgacaa ttccactttg agatcaaccg tagagccttt
     5401 ataaatgata tcaacaatac gaccaggcaa cattccttct gttttttcta cttcggataa
     5461 gctccaaact cgaatatcct ctggacgaac gatcaaatga agccggtcgt tgacttgata
     5521 attgccagtg tttttacaga atagctggat tccttcaatg ttcgttacga tgtcttgatc
     5581 ctttacagtg tgaatctgaa tatcaaatat attcgcttcg ccaataaaca tggcgacatg
     5641 caggttggca ggggtttcat agacagcctt aggcgttcca atttgttcaa tgtggccatg
     5701 attaaagacg acaatacggt cagacattga taaggcttct tcctgatcat gcgttacaaa
     5761 aataaacgtc atattcaagg ttttttgtaa ttgtttcaat tcggattgca ttgccttcct
     5821 aagcctatag tcaagggaac tcaatggctc atctaacaac agtacttgag gtctgttgat
     5881 gattgctcgt gcaatcgcaa cacgttgttg ttggcctcca cttaactgct tcacatttct
     5941 ttcagctaac gattcaagtt gtacaagttt taacgcatca aagactcgct ccttgatttc
     6001 ctggttgggt gtttttttgc agcgcaaggc aaatgcgaca ttttcaaaaa cggataaatg
     6061 agggaaaagg gcataacttt gaaaaacagt atgcacatcc cgtttttgag gtggtaattg
     6121 attaacacat tggccattaa tgaaaatctc accttgcgtt ggttgttcaa aaccagatat
     6181 aagccgaaga agggttgttt ttccgcaacc tgaagggccc agtaaggtta aaaattcacc
     6241 gtgattgacg tttagcgaaa cattattcaa aattggtgta ttgccgtatg atttataaat
     6301 ctgacggatt tcaatgagcg gtgttgtcat ctgctttatt aaataaaaaa ctgcaattat
     6361 gtgtgctgtg actgatcttg tccagtcaat cacaaacttt gtgcccaata ttaaaaaatt
     6421 agtcaactat tgtctatttt tgtgttttgt acatgagcaa aaaaatcagt catgcccgca
     6481 taggcgggca accatctatc aacataatcc aatgcttagg tcaagccact gaggattttt
     6541 ttgttcgatc aagtttattt tccattgcct gttccatttc ttgatacgtt tttctctggt
     6601 tattgcttca taaacatcgg tgtgaatttc ataataaact aaccgatgta cgttgtatct
     6661 tttagtaaat ccttcggtca aacctttttt gtgctgatat attcgttgca caagattact
     6721 ggtaatcccg gtataaaggg tcccatatgc cttactagct aatacataaa caacatattg
     6781 tttctcttcc attattcatc caggttggtt gctataacct gccgcaattt tgcgatcgag
     6841 attatatgat ttttattgta tatcagcgag ttacttcatc ttgttcatat ggtaagccag
     6901 gaatggttgc ccgcctatgc gggcatgacc gattttttgc ttatgcacag agatttcgcg
     6961 gcggatagta tgactttatc cagtaaatca tttattgaat tttccaatca tcaggattgt
     7021 cgtgagataa gtttttgaaa tgttccagaa gttcattcat tccctcacgc cctgttatat
     7081 cttttttgtt ttttgcaaca aactgctttc caaaaagatt gctttgataa cataaatcag
     7141 gattctccat atcaatgact tcagcaaata ccttggctac ttccgttgca ttttgaaaaa
     7201 cagtattttt atggaaccat tgataagctt gttcagtaaa gtttttatag tatttctcaa
     7261 caatatcagc gcgagagcct tcaaatcggg tcaattctgt cgcaattggt ccaggttgga
     7321 aattaattac tttgatattc catggggcca gctcaatcgc caaggattct tgtaatcgtt
     7381 caaatgctgc aagtgctgaa aaatagattg cgacacctgg aatactaacg ggaacagagg
     7441 taaaaatcgt ggatgttgtt gttaataaac cttttttcct ttctctcatt acagggatga
     7501 atcccctgat taaagacaat gggccaaata ctttggtttg aaaggttgtt aatacatctt
     7561 gagtggttgt gctttcaaca gggccaatag ccacattagc agcattgtta accaatgcgt
     7621 caatgtattg atattgactt aagacttctt taataaagcg gtcaattgaa ttttcatcgc
     7681 aaagatccaa tatttttaca tccaaattgc tggagtttgg gaggtttgct ttgttaattt
     7741 ggcttgtttc aagatttctc accgttgcaa tgacttgatg cccgcgtttt agcatttcac
     7801 atacaagatt atatcccacg ccggatgttt ttgaacaacc tgtgaccacc actatttttt
     7861 tatccgtctt tgtcatgtcc ctatcctaat gatcttttag agtaataaac tgagtatagc
     7921 ccttttattt attcagaact tacgaaaacc cccaatctct ttgttaaaaa aaggtttt
//
LOCUS       DATNSN010000147         7968 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.147, whole genome shotgun sequence.
ACCESSION   DATNSN010000147 DATNSN010000000
VERSION     DATNSN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.147"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            20..1648
                     /locus_tag="VAH76_002546"
     CDS             20..1648
                     /locus_tag="VAH76_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEO1387143.1"
                     /translation="MVYYINLTEQPLPQDKEILTQLIKKYLLSGSSQSALFAEHCYTL
                     NDEDGNEIEFRLSRDIYKNQRHDGITEFLILNGPEIGRGTKGVVMQSNHAMYLMYDEL
                     VIFPKENVLKVLHRNFRNAKELIKQESVNYSILSGNSAAELVNLQGGSQGFFMQKEEG
                     CTLEELLEDIDSLQKLTFSDKMELAISFLTELENLHNKGFVHGDLTFKNILYNEESKR
                     LKIIDAGEMKVADERFLAQDILIARDMVSLLFTTNETNQFNASSYWSDEPTARRILYP
                     NLVEAKLNTILNSEYTCISALKSAIEMNYNEYLSQLNPLLQRNELSKMEREASNLHAY
                     KDQMDKQQLFGEIRESKEVFLNMKESLPLVDQYSLKVFSDYLDNICSALNLNNDKTNE
                     KLARAAYDFLTAQINKLFEDNSKSSQPSFESFSEDVQCFLNHINTYLINNPTACSNSI
                     VSTIKLLKQLDNKRSFNPEQSLKNFCGYKEIMIQLLLKPFETPVAEMASVPATVSDFI
                     EKQSDTKRRLQEMKSSANQDKQPIFTPSLMRGPS"
     gene            1878..3191
                     /locus_tag="VAH76_002547"
     CDS             1878..3191
                     /locus_tag="VAH76_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1387144.1"
                     /translation="MEYHRVWRFSYGLVLLLLYASLATAGNALTLKEVTQIALANNKD
                     LKAARYNVSIAKARLLQAGFWPNPSLNLSNNDDRLFTDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAITEIREAERQLSAKVASAFYTAVITEHRIQQVNYLLQINQELVD
                     VIHNRYHAAEISKLDENAARIEYLRIVQEKHLLRSLSISQYATLNQLMGREANSSLSL
                     KMDFAAAKKLPALDALKTQALENRPDRQSIALSIERANADRRLAKAERFADWTVGLGI
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMSLRALDLSI
                     ATEIASNYEQLKALRKSLEETQRVSLQLTIENVKLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKFLQVYVALCTAIGPGKTDGFCSYLEYQSNGDSK"
     gene            3188..4156
                     /locus_tag="VAH76_002548"
     CDS             3188..4156
                     /locus_tag="VAH76_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1387145.1"
                     /translation="MRLQLKIRMMIMTVFFLGLLSSMQVSFAHGGNIEVSEGGTKGPV
                     HLTPEQARMLDIKVVQATHRPMAQLLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKAGQRLATVQSRLVGNPPPSVAVNAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVVAQVYEEDLGKVKVGQEVNVRVLSYPKQTFPGLVTLIEPNLDPLTRTVNVRITL
                     DNKERLLKPGMFVRANVILTHNKAALTVPNTALLQADSKQFVFVQNGGTYKRVDVKVG
                     AVEDDYSEIIDGLVPGDLVVTQGNRELYTLWLSGGRKLQSGQEEHH"
     gene            4157..7357
                     /locus_tag="VAH76_002549"
     CDS             4157..7357
                     /locus_tag="VAH76_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEO1387146.1"
                     /translation="MFTRLIDWSLHNRLLVLALTIILCLLGGYTLRQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYLNRQLVNERIQQVVNRLPPGISPPVMLPVTSAVGWLLKYALVSHTASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLDPKRMLAYGITVEEVRQALATGNQNVPG
                     AFVHQGGTEFVVSTIGRIKTLDDIKKTLIVVRKGVPVTVNNVATVAFGGEIKRGDASF
                     NAEHAVIGTVSKIYGADTLTTTRKVEQALADIKKSLPAGIELNTEVFRQANFIESAIH
                     NLTKALIEGAVIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAENPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYLLLARKTEKSQDA
                     KVAVHALSEDERYHCTQEETVKQAQTETRFVIWLKKHFLTGLNWSLAHCKTVVAISIA
                     AFVFALSLLPFFGTSFLPEFHEGNFIIVMSTLPGTSLNESMRLGKQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVHLNFGKDKTMSPDELLQSIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGESVESILKTVPGVVDINKEQQIN
                     VPQLVIKIDREKAARYGVNIGQISDDVQILLNGMTVSSILEGQRTFDLYLRMDETERD
                     SVKGIQNMLIDAHGAANNQETKIPLRAVADIQLEPQPFAINRENVQRVLVIGFNVQGR
                     DLGSVIQEVQHQVKEKISLPSGYFIQYGGQFESQQQASRVIILFGALAVFIMLVLLHK
                     AFGTFREALLVMFNLPLALIGGVVSLFLASGDMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRSLGREREQVVIQGTLDRLVPVLMTAATAALALIPLLWGSPAGKELQRPLAQVL
                     LGGLLTSTVLNMLVVPTVYNAFEIWHEKRRQLKEIS"
     gene            complement(7464..>7968)
                     /locus_tag="VAH76_002550"
     CDS             complement(7464..>7968)
                     /locus_tag="VAH76_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387147.1"
                     /translation="AKYTTLSEAQTKFVVTLGQGLVVLFIVLTGMYADRIDERHIIKT
                     GLWGYFIAAPVAFLIPQTNSFALILLSQIPYALCNALVGTPIFKLLNDFFPTQIRYSG
                     VSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILLSASIALFILHDKKSYKITS
                     IKLRSDN"
BASE COUNT         2362 a         1446 c         1730 g         2430 t
ORIGIN      
        1 tggcttgtcg gggagaatta tggtttatta cataaatttg acggaacaac ctttacctca
       61 agataaagaa attttaacgc agttaattaa gaaatattta ttgtctggtt ccagtcagtc
      121 cgcattgttt gctgaacatt gttacacgct aaacgatgaa gatggaaatg aaatagagtt
      181 ccgcttgagt cgtgatattt acaagaatca acgtcatgat ggtattactg agtttttaat
      241 tttaaatggt ccagagatag ggagaggaac caaaggcgta gtgatgcaga gtaatcatgc
      301 tatgtattta atgtatgatg aattggtaat tttccctaaa gagaatgtac tgaaggtttt
      361 acatcgaaat tttagaaatg ccaaggagct aataaaacag gagtctgtca attattcgat
      421 tttatcaggt aattcagctg cagaattagt caatttgcaa ggaggaagcc aaggtttttt
      481 tatgcaaaaa gaagaagggt gtactttgga agagcttctt gaggatatag actcacttca
      541 aaaattgaca ttttcagaca aaatggagtt agctatttca tttttaactg agttagaaaa
      601 tctgcataac aaaggctttg tccatggcga tttaactttc aagaatattt tatataacga
      661 agagagtaag cgattaaaaa tcattgatgc aggagagatg aaagtagcag atgaacgctt
      721 tttagcgcag gatattttaa ttgccaggga tatggtatct ctcttgttta caactaatga
      781 aacaaatcaa tttaatgcat caagttattg gtctgatgaa cccacagcca gacgaatact
      841 ttacccaaac ctggtcgaag ctaagttaaa tacaattttg aacagtgaat acacttgtat
      901 ttcagcttta aagtcggcca ttgaaatgaa ttacaatgaa tatctaagcc aactgaatcc
      961 tctattgcaa agaaatgagc tttcaaaaat ggaaagagag gcttcgaatt tgcatgccta
     1021 caaggatcaa atggataagc aacaactctt tggggaaatt cgagaatcta aagaagtttt
     1081 tttaaatatg aaagaaagtt tgcctcttgt agatcaatac agtcttaaag tattttctga
     1141 ttatctggat aatatctgct ccgcattaaa tttaaataat gataaaacca atgaaaagtt
     1201 agctagagct gcctatgatt ttctaacagc acaaatcaat aaattatttg aggataattc
     1261 gaaaagctca caaccaagtt tcgaaagttt ctcagaagat gttcagtgtt ttttaaatca
     1321 tattaatacc tatttaataa ataatcctac cgcctgctct aattccatcg tttccaccat
     1381 taagctattg aaacagttgg ataataaaag gagttttaac ccggagcaat ctcttaagaa
     1441 tttctgtgga tataaagaaa ttatgattca attgttatta aaaccatttg aaacaccagt
     1501 ggctgaaatg gctagtgtac ctgctacagt atctgatttc attgaaaaac aatctgacac
     1561 caaaagaaga cttcaggaaa tgaaatcttc tgcaaatcaa gataagcaac ctatatttac
     1621 cccttcattg atgcgaggcc caagctaaaa aacgtcactg aagtgttaga gcagcctgtc
     1681 atcaattgtt tcactgctct tgtttaattg catttgctgg gagatactaa atcatacaac
     1741 aataaaaaga aggtattaat tgatgactta ctttcgaata aatcacaatc cattactctt
     1801 ggtgtttgca cttgctacag attatttttt taattctatg ttgactgaag atagatagat
     1861 tttttgagct atcgtttatg gaatatcatc gtgtttggcg gttttcttat ggattagtct
     1921 tgctactgct gtatgcctct ctggcaacgg cggggaacgc tttgactctt aaagaagtaa
     1981 cccaaattgc tttagcaaac aacaaggatt taaaagctgc ccgttacaat gtatctatcg
     2041 caaaagcccg gttgctacaa gcaggttttt ggcctaatcc aagtttgaat ctttcaaata
     2101 atgacgatcg tttattcact gatgaaggag agtattcgcg cagtgctggc tttagccagg
     2161 catttcccat atcaggacgt attgcaaaac aaaagacagt ggcgcgtctg gatgtgttga
     2221 aagccattac agaaataaga gaagctgagc gccaactcag tgcgaaagtg gctagtgcct
     2281 tttatacggc agtgattaca gaacatcgca tacagcaagt caattactta ttacagatta
     2341 accaggagtt ggttgacgtt atccataatc gatatcatgc cgcagaaatt tcaaaattgg
     2401 atgaaaatgc ggcacgcatt gagtatttgc gtattgttca agaaaaacat cttttgcgca
     2461 gccttagtat tagtcaatat gcgacactaa atcaattaat gggacgtgaa gccaactctt
     2521 ctttatcact taaaatggat tttgccgctg caaaaaaact cccggcatta gatgctctaa
     2581 aaacacaggc gcttgaaaat cgccctgatc ggcaatctat tgcgttatcg attgagcgtg
     2641 ccaatgctga tcggcgtttg gcaaaagccg agcgctttgc agattggaca gtaggccttg
     2701 gtattcagca ggataaaatt gtggtggaag aaggcccccc tcaaccagcc gatcgtactt
     2761 tgggcgttac actgtctgtt cctctccctc ttttaaatcg aaatcaaggg cgaattttag
     2821 aagcaagtca tacaggtact caggcgatga tgtcattacg tgccctcgat ttaagtattg
     2881 caacagagat agccagtaat tatgagcaac tcaaagcatt acgtaagagt cttgaggaaa
     2941 cacaacgcgt ttctcttcaa ttaactattg aaaatgtaaa attggcccgt gattcttatg
     3001 aaaatgggca aatttctttt ttaaatgtgc tacaagtgca aaaacaacaa aacgatttgc
     3061 aaaccactta tctcaatacg ttagaaaaat ttttacaagt ctatgtggcc ttgtgtacag
     3121 ctattggtcc tggaaaaaca gatgggtttt gctcttattt agagtatcaa agcaacggtg
     3181 atagcaaatg agattgcagc ttaaaattag aatgatgatc atgactgttt tttttcttgg
     3241 tttactttcc tccatgcaag tgagttttgc acatggagga aacatagaag taagcgaagg
     3301 aggcaccaaa gggccggttc atttgactcc ggaacaagcc aggatgcttg atatcaaagt
     3361 tgtccaggca acccatcgtc ctatggcaca attgctgggt ttaaatggtc aaattcaatt
     3421 attacctgat gctcaagctg atgtcagcat tcgtattagt ggcagtgtga cagcgattga
     3481 tgtgaatttg ggggatagtg tcaaggcagg acagcgcttg gctacagtgc aatcacgatt
     3541 ggtgggaaat cctccaccga gcgttgctgt caatgcccca attgctggta tcattgatgc
     3601 caggaacatc aatttaggtc aggctgtcga gccaaacacc gttctttttc atatcagtaa
     3661 tcgcgataaa ttattagtcg tagctcaagt gtatgaagaa gatttaggta aagtgaaagt
     3721 agggcaagaa gtgaatgttc gtgtattaag ttatcccaag cagacattcc cgggcctggt
     3781 cactttaatt gaacccaatc ttgatccgtt gacccgtaca gtcaatgtac gaattacttt
     3841 ggataataag gagaggctac tgaaacccgg aatgtttgtg cgggccaatg tgattttaac
     3901 tcataataag gcagcactca ctgtgccgaa tactgctctt ttacaggcag atagcaagca
     3961 atttgtcttt gtgcaaaatg gaggcaccta caaacgagtc gatgttaaag taggtgctgt
     4021 ggaggatgac tactcagaaa ttattgatgg tctagttcct ggcgatctcg ttgttactca
     4081 gggaaatcgc gagctttata cactttggtt gagtggaggc cgcaaactgc aatcagggca
     4141 agaggagcat cattaaatgt ttactcgcct tattgactgg tctttgcata atcgtctctt
     4201 ggttttggca ttaacgatta ttctttgttt gctgggtggt tatactctaa ggcaaatgcc
     4261 ggtagatgtt tttcctgagt ttgcccctcc tcaagttgta gttcaaaccc aagcgccagg
     4321 catggcaacc caagatgtgg aaacattaat cacttatcct ttggaaagtg ccatcaatgg
     4381 gactccaggg gtagctagtg ttcgctcaaa aacctcagtc ggattatcaa caattaccgt
     4441 ggtatttgat gataaaacgg atatatatct caatcgtcaa ctggttaacg agcgcattca
     4501 acaagtagtc aaccgtctcc ctccaggcat ctccccacct gtgatgctgc cagtcacatc
     4561 ggcagtaggg tggttgctta aatatgcttt ggtgagtcac acagcaagcc ctgaaacctt
     4621 gcgcactatt tccgattgga ctattcgtcc acgcctattg gctttaggcg gagttgcttc
     4681 agtagtttca ctgggcggag aagtaaagca atatcaagta cagcttgatc ccaagcgcat
     4741 gctggcttat ggcattacag tcgaagaagt tcgtcaggcc ttggcaacag gaaatcaaaa
     4801 tgtacctggt gctttcgtac atcagggggg tacggagttt gtcgtaagta ctattggtcg
     4861 tattaagacg ttggacgata taaaaaaaac actgattgta gtccgcaagg gagttcctgt
     4921 tactgttaat aatgtagcca cagtcgcttt tggcggtgaa atcaaacgcg gtgatgcttc
     4981 tttcaatgca gaacatgctg tgattggtac tgtttccaaa atttatggcg ctgatacatt
     5041 aaccacaaca cgtaaagtgg agcaagcttt agctgatata aaaaaatcct tgcctgcagg
     5101 tattgaatta aatacggaag tatttcgcca agccaatttc attgaatcag cgattcataa
     5161 tttaaccaaa gcccttattg agggtgctgt tattgtgatt gctgtattgt ttcttttttt
     5221 gatgaattgg cgagcctcgt ttattacctt tctctctatg cccgtttcct ttatcgtagg
     5281 gatcttggtc cttcattatt tcggttttgg tattaactcc atgactttgg gaggaatggc
     5341 gattgctatt ggtgaggtgg tggatgatgg gattattacc gtagaaaatg tggtgcatcg
     5401 tttacgactt aatcgtttgg cagagaaccc cttgcctgcg attgaaattg tttttgatgc
     5461 ggttttggaa attcgcagtt cagtggttta tgcgaccatt atcattagcc tggtgttttt
     5521 acccatcttc tttttgtcag gaattcccga gcggattttt agcccattag ccattgctta
     5581 tattgcctcc gtattaggtt ctcttgtcgt ttctattact atggtaccag ccctgtgtta
     5641 tctcctttta gcgcgaaaaa ctgaaaagag tcaggatgct aaagtggctg tacatgcctt
     5701 atctgaagac gagcgttatc attgcacgca agaggaaacg gttaagcagg ctcaaacgga
     5761 aacgcgcttt gtgatttggc ttaaaaagca ttttttaact ggtttaaatt ggtcactggc
     5821 ccattgtaaa acagtcgtag ccatctcaat cgcagccttt gtttttgctt taagtttact
     5881 tccttttttt ggaacttctt ttttgcctga atttcatgaa ggcaacttta ttattgtgat
     5941 gagcactctt ccgggaactt cattgaatga atccatgcgc ttggggaagc aagtaagtaa
     6001 gactttgttg aaatatccgc aagtgatttc tattgcccaa agagcagggc gcagtgaatt
     6061 ggatgaggat gctttaccac cgaatattag tgaatttgat gtacatctga attttggtaa
     6121 agataaaacc atgtctcctg atgaattgtt acaatctatt cgagctgatt tagcgaatat
     6181 tccaggagcg ttatttaatg tggggcagtt tattgcccat cgcatggatg aagtgttatc
     6241 aggagtcaga gcacaagtag ccgtgaaact ctttggcgat aatcttgcta cccttaatga
     6301 attgggtgaa tcggtagaat ccattttaaa aaccgtccca ggggtagtag atattaataa
     6361 agagcagcaa atcaatgtac ctcagctggt cattaaaatt gatagggaga aagcggctcg
     6421 ctatggtgtc aacattggtc aaatttccga tgatgttcaa atccttctca atggtatgac
     6481 ggtatccagt attcttgaag gccagaggac gtttgatttg tatttgcgta tggatgaaac
     6541 agagcgggat agtgttaaag gcattcaaaa tatgctgatt gatgcccatg gtgcggccaa
     6601 taatcaagaa acaaaaatcc ctttgcgtgc ggtggctgac attcagttag agcctcaacc
     6661 gtttgcaatt aatcgagaaa acgtgcaacg tgttctggtt attggtttta atgtgcaagg
     6721 gagagatttg ggtagtgtga ttcaagaagt acaacatcag gttaaagaaa agatttcatt
     6781 acctagtggt tatttcattc aatatggcgg ccagtttgaa agtcaacaac aagcatctcg
     6841 tgtgattatt ctttttggtg cgctggcagt ttttattatg ttggtacttc tgcataaagc
     6901 tttcggcaca tttagggaag ccctgttggt gatgttcaac ttacccttgg cattgattgg
     6961 tggagtcgtt tctttatttc ttgccagcgg tgacatgagt gtcgctgcca tgattggttt
     7021 tattactctg tttgggattg cagcgcgtaa tgggattatt ttagtgagcc actataatca
     7081 attgcgctct ctggggcgag aacgtgaaca agtggttatc cagggaaccc ttgatcgcct
     7141 ggtacccgtg ttaatgacag cagcgacagc ggctctagct ttaatacccc tattgtgggg
     7201 ttctcctgcc ggtaaagaac tccagcgccc tttggcgcag gtgctattag ggggattatt
     7261 gacttctacg gttttaaata tgcttgtagt acctacagtt tataatgctt ttgaaatttg
     7321 gcacgaaaag agaaggcaat tgaaagaaat atcttaatca gagccatgat gactctaaca
     7381 atgggggcta ttaagaagca attgtcacaa attgattaga tgcttgcgta gacatgtttt
     7441 tgcaatggtg ataagtagtt tattcaatta tctgatctta attttatgga ggttatcttg
     7501 taactctttt tatcatgcaa aatgaacaag gcaatactgg cagataaaag tatatagaat
     7561 attggcgcag ttgttaattt gaatgaatat tgtaaataat tggcaaccaa tggcgctgtt
     7621 cctccaaaaa tagccatgct tactccccat gcaacagaaa caccgctata tcgaatctga
     7681 gttggaaaaa aatcattcaa tagtttaaaa attggtgtgc cgaccagagc attgcataat
     7741 gcatagggaa tctgcgataa gagaattaat gcaaaagaat tggtttgagg tatcaggaaa
     7801 gcaacaggag ccgcaataaa atacccccac aaaccggttt ttataatatg tctttcgtca
     7861 attcgatcag catacattcc tgttaaaaca atgaatagta caactaatcc ttgccctaag
     7921 gtaacaacga atttggtttg agcctctgac aacgtagtgt attttgcc
//
LOCUS       DATNSN010000148         7949 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.148, whole genome shotgun sequence.
ACCESSION   DATNSN010000148 DATNSN010000000
VERSION     DATNSN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.148"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            120..548
                     /locus_tag="VAH76_002551"
     CDS             120..548
                     /locus_tag="VAH76_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387148.1"
                     /translation="MYFSRCISHGAIAGIIAGIVFTFFLVMGGMLETLGSMINMPTKG
                     GGLLVHAVMSIGSGIAFALVLDWLMHSWISAILLGLLFGFAMWVGGPMTVLPYFASGV
                     PLFAKWTMEGFKQNIPPLVGHLVYGLILGVTYYAFKRRNK"
     gene            complement(685..1533)
                     /locus_tag="VAH76_002552"
     CDS             complement(685..1533)
                     /locus_tag="VAH76_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387149.1"
                     /translation="MCKEINIIWVGPQRLPYKYLQHLKKWHNLNPDHQINFITDEHSS
                     SIQLPGINTIHWKQCFDLTDVRQEFLLREAERALREKAYATLSNIVRKVKIFNDGGYY
                     FDTDIVPIEKLPAITENTPFLSQPKASYGAEFAKHRFVVSALYGSKHSPVFNEALTVM
                     HETYQVKSLQDAIKENPEKSDYLRSYYSASLMLSRSVKRLYTNEQDYQKMIEDCQNQF
                     GSPTAFQIIQDGSWGEGLNAVLMESKHLAATAIQRFFRAAQALNQVNSNQSTSHMQSP
                     EKPRFF"
     gene            complement(1709..3967)
                     /locus_tag="VAH76_002553"
     CDS             complement(1709..3967)
                     /locus_tag="VAH76_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEO1387150.1"
                     /translation="MFAEIPKIQEQLHNVLEYSQSMTSLWMQSIDKSLEQTNKVTQSL
                     INLNQQQIYPYPNILNDAIEYTVDFMQRSILFWDIMRKRGNQYLKHEQEGQPPVLIFS
                     YNMLMDGRHFERPVNYALVEIIPPEGIQIDPAKRPYVIVDPRAGHGAGISGFKDASQV
                     GLALRAGHPVYVIIFFPVPEPHQTLVDVTAAHEKFLTEVALRHPQSPKPCLIGNCQGG
                     WAILTLMAANPDVAGVAVVNGAPLSYWGGENGKNPMRYIGGIWGGSWIAQMAGDLGNG
                     KFDGANLVMNFEMANPKVTYWSKYYNLFANLDKEEIRFLNFERWWGGFSLLNVNEMRG
                     IIDNLFIGNKLVHGKIPLGQSGNNLDLRNISVPVIIFCSEGDNITPPQQALNWVSDLY
                     SNTLEIKLDGQVIVYLIHKSVGHLGIFVSSEVAKKEHNQIIDLLNYIEHLAPGLYELK
                     LNEIKENPKSPPHYLAYIEERSINDIRKGQENNVAIFNLVRMVSEYNAMSYDLFMAPI
                     IRNISNEYTAELIRKLHPLRQNQYLLNDLNPLVHPVSWISPFIRQNRIKISENNPFLT
                     QQLNFSKLINALWDFFSASRDNTVELMFYAVYGYIQLLAPFDPRRDMIVHSPEKDNQE
                     KMTQSIIAHISDGGVPEAILRILLLLVKIQGYIIGANLPDVIQKLRECNALKHLDKNG
                     FKQIVHSQTILIEYDPELAFNTIPHLLKSQEERSMVIQFIEDILKSLKVSPSEEYKKK
                     FQRIKELLQNQV"
     gene            4501..5949
                     /locus_tag="VAH76_002554"
     CDS             4501..5949
                     /locus_tag="VAH76_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2160 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387151.1"
                     /translation="MFLANQSLDFAWLSMQMFLINLLKDKIMRYAVTYCAMDHEFSGN
                     PFWHSCILLSQWDDNGKIEVVDNWGFYGVPSTVRDTWLSKLKIKLGLDVDLKGNHGML
                     RHEELRYLDVGYGLHGVTFELTKEKFDLLQLKCQTMLEEQKQAVKEVVESQGIAGKQK
                     YRIYEHEEYSPLIFALEKIKAKQKGHQPRLKPFELNLSFTLWGPALNQSYTCKSLMVD
                     LLKGILTQEQIARITEAGKHPTVPRYSGKLERIYLHSSGPLREHKKSSGAIVHYRDLQ
                     DEGVKLHWTLPPQEIEALSGDTIKLLEISEEYCDEVKQAISKLQKLEWLFIDAKLPEK
                     YKPYQDDLVARIRQCYEAFAQVEPKKAKSTVTGWMGFAFSLLSLPRDLDEKCLLQKLN
                     NAKYLINSLYMAIVDHWKIYDDWPSETKADSTDYNPLEALAAYLCEEDKKKLCKIIGR
                     NYLEPSSEEEFDDIEESGWMNSELGHTSASAM"
     gene            6234..7355
                     /locus_tag="VAH76_002555"
     CDS             6234..7355
                     /locus_tag="VAH76_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEO1387152.1"
                     /translation="MNALTSWIKAWSQKFFPNQKKNENKEEEADLTQEKKVRPNSDFY
                     ENYFSLVAAGTRLKLIEAMFGVNLFSLFENKAWVFEKDIVEKLQLMPIRARKWLHLLS
                     SEHFLIQIKVNGQQAYQLPEEFIELIHSEEFPAMEMFFKTWLVSAEENLTDVLRFGKV
                     KTSVSWPPKTQKEAEWLEDWMTRTVDQPTDCLLKSINFKKVNKFLDVGGGDGTMACTF
                     VKKYPHLKAAVYNLPMSAQMARNNIESRKLNHRVHVIEGDFIEEDAFPVGYDLILFTR
                     VLFDWDDQVCRKLLRMAYQALPKNGLVGICEFYKEDNNDICLASEYRYIFHDDFGVNV
                     MKRASVYQNMLEKIGFTIVLPHKVKEMHFYYCSLILARK"
     gene            complement(7384..>7949)
                     /locus_tag="VAH76_002556"
     CDS             complement(7384..>7949)
                     /locus_tag="VAH76_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1387153.1"
                     /translation="FGSIFHQGRSLCANKVNKNAGLNASVLELGVGTGLSLPFYRPDL
                     NITGIDISEKMLEKAEKRIVKKKLTTHINLKIMDAANLEFPDNHFDFVVAMYVASVVP
                     DIDTFLKEISRVCKPSGEIIFVNHFASEKPILRFFEKQLSHIDNFVGFNSNFSIHSIL
                     NYKEFKLLETQKVNLFGYWKLLHCKIN"
BASE COUNT         2405 a         1335 c         1615 g         2594 t
ORIGIN      
        1 tgagggagtt tagataaact aaatgaccga attaaaaaca ttttttaaca tagagattgg
       61 ggtttttcgt aagtcctgtt tgaatggtaa actgggatct atttaataag gagattggca
      121 tgtatttttc aagatgtatt tcgcatggcg ctattgcagg aattatagca ggaattgttt
      181 ttaccttttt tttagttatg gggggcatgc tcgaaacatt gggaagtatg attaatatgc
      241 caacaaaagg aggaggcctt ttggtgcatg ctgtaatgag cattggcagc gggatcgctt
      301 ttgccctggt tctggattgg ttaatgcatt cctggatctc agccattctt cttgggttat
      361 tgttcggttt tgcaatgtgg gtcggcggcc caatgaccgt gttaccctat ttcgcttcag
      421 gggtgccttt gttcgcaaaa tggactatgg aaggatttaa acaaaacata ccccctttgg
      481 ttgggcatct tgtgtatggg ttgatattgg gtgttactta ttatgctttc aagcgcagga
      541 ataagtgaca tcatttttca gcgtaacaaa ttgattttat taaagatata atgacataaa
      601 aaggtggtga ttgtttttta taggaatcgc cacctcaatt tagcaaaaac aattgcatgc
      661 ctagcaatca agcccatggc ctcttcaaaa aaatcttggt ttttcagggg attgcatgtg
      721 actcgttgac tgattggaat tcacttgatt cagagcttgc gcagctcgaa aaaatctttg
      781 tatggcagtt gctgccaggt gtttgctttc catcaaaaca gcatttaatc cctctcccca
      841 tgaaccatct tgtataattt gaaatgcagt agggctccca aactgatttt ggcaatcttc
      901 tatcattttt tgataatcct gttcgtttgt ataaagtctt ttaacagaac gggataacat
      961 aagagaggct gaataatagg agcgaaggta gtctgatttc tccggatttt ctttaatggc
     1021 atcttgcaat gattttactt gataagtttc atgcatgacg gtcaatgctt cattgaatac
     1081 tggagagtgc ttgctaccat aaagcgcaga aacaacaaac cgatgcttgg caaattctgc
     1141 accataactt gctttaggtt gtgacaagaa aggggtattt tcagtaatag caggcaattt
     1201 ctctatagga acgatgtcag tatcaaaata atacccgcca tcattaaaaa ttttaacctt
     1261 gcgaacaata ttcgataaag tagcataggc tttttccctg agagcacgct ccgcttctct
     1321 cagcaaaaat tcttgtcgta catccgtcag atcaaaacat tgtttccagt gaatggtatt
     1381 tattccggga agctgaatag aagaagaatg ttcatcagtg atgaaattga tttgatggtc
     1441 tgggtttaaa ttatgccatt tttttaaatg ctgtagatat ttgtagggaa ggcgctgtgg
     1501 tcctacccaa atgatattaa tttctttaca catattgtgt ttaccaccaa ttagaaaaaa
     1561 ctgtttttat agcaatgtat tcagcatgtc aataaaataa acaattgagg tgggggattt
     1621 catttgtctg aaagatgaag gattgatttt ccaataggat acctataggt ttaggcttga
     1681 ttgagagcgg tatggcccaa tttggaaatc aaacctggtt ttgtaacagt tcttttattc
     1741 tttgaaattt ctttttatat tcttcggaag gggatacttt taaagatttt aatatatctt
     1801 caatgaattg aatgaccatt gagcgctctt cttgtgattt cagtaaatga ggtattgtat
     1861 taaatgcaag ttcaggatca tactctatca atatggtttg tgagtgcaca atttgtttga
     1921 atccattttt atctaaatgt ttaagagcat tgcattcacg tagtttttga atgacatcag
     1981 gtaagttagc gccaatgata tagccttgaa ttttcactaa taatagtaat atacgcaaaa
     2041 tggcctctgg tacacctcca tcactaatat gagcaataat tgattgagtc attttttcct
     2101 gattatcttt ttcaggggaa tgaacaatca tatcccttct aggatcaaat ggagctaata
     2161 attgaatgta gccatagaca gcataaaaca ttagttccac agtattatca cgagacgcgc
     2221 tgaagaaatc ccataatgca tttataagct ttgaaaaatt taattgctga gttaaaaagg
     2281 gattattttc agaaatttta atgcgatttt gacgaataaa aggtgatatc catgatacag
     2341 ggtgcactaa tggattcaga tcattcaata aatactgatt ctgccttagt gggtgtaatt
     2401 ttcgaatcaa ttctgcagtg tattcgttac ttatattacg gattatcgga gccataaaca
     2461 agtcatagct catggcattg tattcagata ccatacgaac aaggttgaag attgctacat
     2521 tattctcctg tcctttcctg atgtcgttaa tagagcgctc ttcaatataa gcgaggtaat
     2581 gtggtggtga cttgggattt tcttttattt cattcagttt tagttcatat aaacccggag
     2641 ccaaatgttc tatgtaattt aataaatcaa taatctggtt atgttctttt ttagccacct
     2701 cacttgagac aaaaatacct aaatgaccaa cgctcttatg gataagataa acaataactt
     2761 gcccatccaa tttgatttcc agagtatttg aataaagatc agaaacccag tttaaggctt
     2821 gttgaggtgg tgtaatgttg tcgccttccg agcagaaaat aataacaggc acgctaatgt
     2881 ttcttaaatc caggttatta cctgattgcc ccaagggtat tttgccatgg accagtttgt
     2941 taccaataaa caaattatca attattcctc tcatttcatt cacattcaaa agagaaaaac
     3001 ctccccacca tcgctcaaag tttaaaaatc ggatttcttc tttatcaaga ttggcaaaga
     3061 gattgtaata tttgctccag taagtcactt taggattagc catttcgaag ttcattacca
     3121 aattagcccc gtcaaatttg ccgttaccca aatcaccagc catctgtgcg atccagctcc
     3181 ctccccaaat tcctccgatg tagcgcatgg gattttttcc attttcccca ccccaatagg
     3241 aaagaggagc gccattgact acagcgaccc ctgctacatc tgggttagcg gccataaggg
     3301 ttaaaatagc ccatcctccc tgacaattac caattaagca gggtttggga ctttgagggt
     3361 gacgcaaggc tacttcagtt aaaaattttt catgggctgc ggtgacatcc accaatgttt
     3421 gatggggttc aggaacaggg aaaaagataa taacgtaaac ggggtgtcct gcccttaagg
     3481 caagtcctac ttgtgacgcg tctttaaaac cgctgattcc tgcaccatgg cccgcccgcg
     3541 gatcaacaat cacataaggc ctttttgctg ggtcaatctg gatgccttca ggggggatta
     3601 tttccaccag tgcataattt acaggtctct caaaatgcct gccatccatg agcatattat
     3661 aagaaaaaat gagaacagga ggttgtcctt cttgttcatg cttgagatat tgattgcccc
     3721 gtttgcgcat gatgtcccaa aacagaatgc ttctctgcat gaagtctacg gtatattcaa
     3781 tggcatcatt taatatgttg ggataaggat aaatttgttg ctgatttaag ttgattaaag
     3841 attgcgttac cttgtttgtt tgttcaaggc ttttatcaat tgattgcatc caaagagaag
     3901 tcatggactg ggaatattca agaacattat ggagttgctc ttgaattttg ggtatttcgg
     3961 caaacatctt tttctcctgt ggcctctcaa ttcgaggttc tttaactcca tatcgttaac
     4021 aaatgttgac tatcatggta cgttgccatc attctcttct tttttcatat tttcgtaagg
     4081 cggttttctc tttagagaaa tatcctcttc aaacgataat aaaaccattc gcctttttta
     4141 gccttttaac acgttccggt ttgctggcca gtgcaaatag gaggcattgt gcattgtgca
     4201 tcgagcatgt aaacccattt gttaatcact tttaaatcat tatagacagt tattgtcaaa
     4261 acagtaaatg caccgtgttc actgaaattc gattgttacg ctttggattt gtatcaattt
     4321 tgtttttagt ttgaaaggtt aaaaatttat ttctactaat ttatatgtta ggcagttggg
     4381 tttgctttta ttgggaaatt ccaggggtta tctcattatc tgtttagccg ttgacggcaa
     4441 tatggggatc tgtaatagtt tcataggatt gtattatgtg agtcaggttt aagcagttca
     4501 ctgttcttag ctaatcaatc tctagatttt gcctggttat ccatgcagat gtttttaatc
     4561 aatcttctta aggataaaat aatgagatat gcagttacct attgtgcgat ggatcatgag
     4621 tttagtggta atcctttttg gcatagttgc attttattat ctcaatggga tgacaatgga
     4681 aagattgagg tagtcgataa ttggggtttt tatggggtgc catctactgt tcgtgatacg
     4741 tggttaagta aactgaaaat caagttgggt ctggatgttg atttgaaggg caatcatggt
     4801 atgttacgac acgaagaact gcgttatttg gatgttggct atggtttaca tggagttact
     4861 tttgagttaa ccaaggaaaa atttgattta ttgcagttga aatgccaaac aatgttggaa
     4921 gaacagaagc aagcagttaa ggaagttgtt gaatcacagg ggattgctgg taaacaaaaa
     4981 taccgtattt atgagcatga agagtattcc ccgttaattt ttgctttgga aaaaatcaag
     5041 gcaaaacaaa aaggccatca gccaaggctt aaaccatttg agttaaattt gtcatttacc
     5101 ctgtgggggc cggctttaaa tcaatcctat acttgtaaat cactgatggt tgatttatta
     5161 aaaggcatac tgactcaaga gcaaattgcc cgaattactg aagcaggaaa acaccctacc
     5221 gtaccaagat acagtggtaa attggaaaga atttatttgc atagcagcgg accattacgt
     5281 gaacataaaa aatcttccgg tgcgattgtt cattatcgtg atttgcagga cgagggagtc
     5341 aaattacatt ggacattgcc tccacaagaa attgaagcgt tatccggaga taccatcaaa
     5401 ttactggaaa taagcgagga gtattgtgat gaggtaaaac aagcgataag caagctgcaa
     5461 aaattggagt ggttatttat tgatgccaaa ttaccagaga aatataagcc ttaccaagac
     5521 gatttggttg ctcgtattcg gcaatgttat gaagctttcg ctcaggttga acctaaaaag
     5581 gctaagtcaa cagtcacagg ctggatggga ttcgcctttt cattgctctc tttgccaaga
     5641 gatcttgatg aaaagtgtct tttgcagaaa ttaaataatg ctaaatatct gattaacagt
     5701 ctatacatgg ctattgttga tcattggaaa atttatgatg attggccatc tgaaactaaa
     5761 gcagattcta ctgactataa tcctcttgaa gctttggctg cttatttatg cgaagaggat
     5821 aagaagaaac tttgtaaaat tattggacga aattatctgg agccttcgtc agaagaggag
     5881 tttgatgata ttgaagagag tggatggatg aattcagagt tagggcatac ttctgccagc
     5941 gccatgtaaa aaagcctgac tgacagccat ctttttaaag ctgcaagtgc ggactatgag
     6001 actggatcat atctgcattt gcatacttat tgttcccatt gtgttatttg aggaaaccag
     6061 ctgaacgagt cttttctaca gacctccaat ctccttgtta aaaaggagtt taattcggtc
     6121 atttagttta tctagactct ctcattaaaa acattttttg ccttgaccct ggtttttttc
     6181 gtaagtcctg ttctattctc atattaatag tactcttaaa agaatattgt gggatgaacg
     6241 ctttaacatc gtggataaag gcatggtcac agaaattttt ccccaatcag aaaaaaaatg
     6301 agaataaaga ggaagaggct gatttaaccc aagaaaaaaa ggtgaggcct aattccgatt
     6361 tttatgaaaa ttatttttct cttgtagccg ctggtaccag acttaaactg attgaagcca
     6421 tgtttggtgt caatttattt tctttatttg aaaataaggc gtgggttttc gaaaaggata
     6481 ttgtagaaaa attgcaatta atgccaataa gggccaggaa gtggctgcat cttcttagtt
     6541 cggagcattt tttaatacaa attaaggtta atgggcaaca agcttatcaa ttacccgagg
     6601 aattcattga gttaattcac agtgaagaat tccctgccat ggaaatgttt tttaagacct
     6661 ggcttgttag tgcagaagaa aacctgactg atgtattgcg ctttggaaaa gtcaagacga
     6721 gtgtttcctg gcctccgaaa actcagaaag aagcagagtg gcttgaggat tggatgacta
     6781 gaacagttga tcagcctacc gattgtttat taaagtctat caattttaaa aaggtcaata
     6841 aatttttgga tgtgggtggt ggtgacggaa ctatggcgtg tacttttgtt aaaaaatatc
     6901 cacatttaaa agcggcagtc tacaatttgc caatgtctgc acagatggca agaaacaata
     6961 ttgaatcgag gaaactaaac catagagtgc atgtgattga gggtgatttt attgaggaag
     7021 atgcctttcc ggtagggtac gatctgattt tatttacaag agttttgttt gattgggatg
     7081 atcaggtatg tagaaaatta ttaagaatgg cttatcaggc cttgccaaag aatggattag
     7141 tcggtatttg cgaattttat aaagaagata acaacgacat ctgccttgcc tcggaatatc
     7201 gttacatttt tcacgatgat tttggtgtga atgtgatgaa aagggcctca gtatatcaga
     7261 acatgttaga aaaaattggt ttcactatag ttctacctca taaagtaaag gagatgcatt
     7321 tttattattg ttctttgata ttggctcgaa agtaaaaatt cttatctgta tttttgtaaa
     7381 gcttcaattt attttacagt gtaataattt ccaatagcca aaaagattga ctttttgagt
     7441 ttccagcaat ttaaattctt tataatttaa aatggaatgg atagaaaaat tcgagttaaa
     7501 tcctacaaaa ttatcaatat gcgacagttg tttttcaaag aagcgcagga tgggtttttc
     7561 agaagcaaaa tgattcacaa atattatttc gcctgatggt ttacaaacac gtgagatttc
     7621 ttttagaaaa gtatcaatat cagggacaac agacgcaaca tacatggcaa caacaaaatc
     7681 aaaatggtta tctggaaact ccaaattagc ggcatccata atttttaaat taatatgggt
     7741 tgttaatttt ttcttgacga tacgtttttc agccttttct aacatttttt ctgaaatatc
     7801 gattccagtt atgttcaaat ctggacggta aaagggtaaa gataaccctg tgccaacccc
     7861 aagctctaaa acagaagcat tcaggccagc atttttatta accttattag cacatagaga
     7921 gcggccttga tgaaagatag atccaaaaa
//
LOCUS       DATNSN010000149         7784 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.149, whole genome shotgun sequence.
ACCESSION   DATNSN010000149 DATNSN010000000
VERSION     DATNSN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.149"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            92..787
                     /locus_tag="VAH76_002557"
     CDS             92..787
                     /locus_tag="VAH76_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO1387154.1"
                     /translation="MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLV
                     QIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELK
                     PNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSEIKNYAYNEIELAIQDLSAGKI
                     GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNG
                     TLSRLQHKWISTT"
     gene            971..4402
                     /locus_tag="VAH76_002558"
     CDS             971..4402
                     /locus_tag="VAH76_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO1387155.1"
                     /translation="MKILKTTEKKSRKPTPKVNRALPLQLVQNTEPDRTYYLRSTVEG
                     IGSFLSTRVRELYNTLAMTFSLDSMDWSSFKKYIWPTISSGAALLLGDYLTRSMSEYQ
                     TGSPEYDFIFKFLFGTAILGYCALNRITDRKFTSQVELSAAVASEVMAITYMYSSVFK
                     GTTYLLNDSISDEYSLIAGLGTSALLVPSGISYLTQKIQEFLVRIQYNKGAQRSDTAV
                     ISSGLTPISNLYFQVNHFIASELMISSRAFQLGLISHNIRYADRIMQKFRNLPALLAD
                     LAPSTIKKMRIQQQKLEHDYEYNHKLHQVLRFTADGPMFISIPRYQLRTGDLVYCDSS
                     IDLACVPVSGELLALRRDLKGEFTQTLEQKKFSVNLKAQNGEDVWIEHETKPDFTSHY
                     KKVDLHAVRDGKQAGVLVGDKLNLYGNDNIFIQIKPEKELLLSSNYEKKAVINEIIAE
                     RKQRSVLYSILGSVIMAAFLQRDITAMPAETLRLMFTLFQTMIPFSEAFLRETVNSRL
                     MKKLNSELGDQPFGTIDALRVVDLCNALGGYYRDRFPNGVAIISDKTGTLTTTRMDVL
                     GLWTNDMAPEVQKTLKEKEGLLLPEETQWLQTFEVFCNAFTNNKKELEPEEHAILELF
                     QSLLDNRQCLEVITLGNNHFKKEMISKDIKKEIETFHLGLYRTFGGRFTLVQDGSDHY
                     LVFCGVPKQEAFKETPLLKAYTTMQSRTGVLSRDWCLARGKLSEREFTILKELFSKDD
                     KKQIEHFILQNKLLLENLMYYGTFIIDNPVKKGAEKFIAQCREVSVPVFVATGDTTKA
                     AENIAKVLCPANAGKIVTLRADQIDEKGVEFLNEENYPSDSTVIFSGINEAILNRFQK
                     LMDREGAKRPVIIFAEMSTEGKGILARYLKDHHYFVVANGDGTNDVMMMKNSNVVIAH
                     HSDDGTFAPGVDGLTNISEEQLRRLFGSQKTFYELFDINLPKSLFVQKFAPLANSQEK
                     PTMALALKSGKMTFDLAKTVGADVTEMNQQHWYSVVFDLLWLWIAFYEINESSDLPMD
                     NRNINASRLISNTMGIALIIAVFQSLANYALFNESTNLTSMLIMLSFLPLILKSIFSG
                     FRMVQDSLYPQVEVTEVEEEGDTSTNGSVLSYFGTFFSRKPVKQLEGLNNAPALK"
     gene            4662..6356
                     /locus_tag="VAH76_002559"
     CDS             4662..6356
                     /locus_tag="VAH76_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO1387156.1"
                     /translation="MHDALPLITTIAMGFALALILGFIAVRLKMPALVGYLLAGVTIG
                     PFTPGLMANVEIAQELAEIGVMLLMFGVGLHFSLSDLLRVRKIAIPGAIVQMIVATIL
                     GGGTAIMWGWDYGSALIFGLSLSVASTVVLLRALEERGTLESINGHIAVGWLLVEDLA
                     MVIVLVLIPPLSHWLGDNSSVTIHRPLWIVLGLTLIKVTTFIALMFLVGRRFFPKLLW
                     QIARTGSRELFTLCIITAAISIAYVASKLFGVSFALGAFFAGMIMRESSFSHRAAEES
                     LPLRDAFAVLFFVSVGMLFNPAVLVEQPLQVLTVVSIIIIGKSIAAFILVLAFRYPIQ
                     SALIISASLAQIGEFSFILAELGIRFDMLPEAGRSYILAGALISIAINPLIFKLINPV
                     HAWVQSHPKLVGILERTDDPLAELPRTTKKEYLSGQVVLVGYGRVGMHIAQLLSKKVV
                     PFVVIDENRELIERLRASGIPAVYGDASEPSVLIQGHIARASMLMTVTSDTIHVRKMI
                     QCAYKLNPDIEIVVRTHNEEEAELLQKEITGKVFFAEGEIAKSMGHYALNRYGKSP"
     gene            6517..7441
                     /locus_tag="VAH76_002560"
                     /pseudo
     CDS             6517..7441
                     /locus_tag="VAH76_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7632..>7784)
                     /locus_tag="VAH76_002561"
     CDS             complement(7632..>7784)
                     /locus_tag="VAH76_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO1387157.1"
                     /translation="DNMDELQKDLDEWLHYYNNERTHQGKMCCGRTPIQTLMDSKQIW
                     MEKFIN"
BASE COUNT         2404 a         1329 c         1596 g         2455 t
ORIGIN      
        1 tcatttaaat caatgacata aaaaatattt ggctgatttt tttctgatta atctatactt
       61 agtgaatcga ttccaaataa tagttatatc tatgattcta aagccgggaa aattattggt
      121 ggctactgct tatccagatc caccgtttga tattgagcca ggtgatggtt ttgatggtga
      181 tttaataaaa gaaatttgtc gaattttaaa tttgcagtta gtccagatca aatatgaggg
      241 agaaaatttt aatgatattt ttttaggctt ggctaataaa gaatacgatg ttgtcatttc
      301 aggaacgact attacaccaa accgatcaaa acaagtgctt ttttcgaatc cctatttaga
      361 gtttaatcag ggtatcgcgg ttaatactga attaaagcca aatgtttctt cttttgaaga
      421 tttgaaaggt ttggtggctg gtatacagaa aggcaacacc tcagacgaag ttgctcagga
      481 tttattacgt aagaacatac tcagtgaaat taaaaattat gcatacaatg aaatagaatt
      541 agccatacag gatctgtctg ccggtaaaat tggtttggtc attaaattat atcctgttat
      601 ttcctgttta attcgaaact atacaaacct ggctgttcct ttgcaggctg aaacacatga
      661 aaaacttggt atcgcggttg ctaaagataa ttttaaactc cgggataaag tcaatcttat
      721 tttggccgat cttgtgcaaa atggtacttt aagtcgcctg caacataagt ggataagtac
      781 aacctagggt aaattaggcc aatgtcagtt tcaactcaag aaataacctt atttaaggaa
      841 tcttattttt cttgaatcgt tctgtaatta tttttcaaat cctcttgaac ttttatacag
      901 ataagttaat tatcaggttg agctctaaag atccaataga gtgttattgg agtacaaaag
      961 gatttataac atgaagatat taaagacgac cgaaaaaaaa tcgcgaaaac ctactcctaa
     1021 agtgaacaga gctttaccgc ttcaattggt acaaaatact gagccggatc gaacgtatta
     1081 tctgagaagt actgtagaag gcatagggag ttttctttct acccgtgtaa gggaacttta
     1141 taacacttta gccatgactt tttctttaga ctctatggat tggagcagtt tcaaaaaata
     1201 catttggcca acgatatcct ctggagcagc attgctgctt ggtgattatc taacccgatc
     1261 catgagcgaa tatcaaacgg gaagcccgga atatgatttt atttttaaat ttctttttgg
     1321 tacagcgata ttagggtact gtgctttaaa tcggataact gacaggaaat ttacttccca
     1381 ggttgaatta tctgctgcag tagccagtga agtcatggca ataacctaca tgtatagttc
     1441 ggttttcaaa ggcactactt atttattgaa tgatagtatt tctgatgaat attcattaat
     1501 tgctggacta ggaaccagcg cattgctggt tcctagcgga atttcttatc taacacaaaa
     1561 aatacaagag tttttagtgc gaatccaata caataagggt gcgcaacgct cggatacagc
     1621 agtgatttca agcgggctca cgcccatatc caatctttat tttcaagtca atcattttat
     1681 tgccagcgaa ctgatgatca gttcacgcgc atttcagctg gggctcattt cacacaatat
     1741 tcgctatgcg gatagaataa tgcagaaatt tcgtaacttg cctgcattat tagcggattt
     1801 ggcaccatcg acgataaaaa aaatgcgtat ccagcaacaa aagcttgagc atgattacga
     1861 atacaatcat aagctacatc aggttttaag atttacagca gatggcccaa tgttcataag
     1921 tatcccaagg tatcaattac gcacagggga tctggtttat tgtgatagca gcatcgattt
     1981 ggcttgtgta ccggtttctg gggagctgct tgctttaagg cgcgatttaa aaggagagtt
     2041 tactcaaacc ctggaacaaa aaaaatttag cgttaacctg aaagcccaaa atggggaaga
     2101 tgtttggata gaacatgaaa ccaagcctga ttttacctcg cattataaaa aggtggattt
     2161 gcacgccgtt agagatggca aacaggcagg agtattggta ggcgataaat taaatttata
     2221 tggtaatgat aatattttta ttcaaatcaa acctgagaaa gagcttttat taagcagtaa
     2281 ctatgaaaaa aaagcagtta ttaatgagat tattgccgaa cgaaaacaaa gaagtgtttt
     2341 atattctatt ttaggctccg ttatcatggc cgctttttta caaagggata ttactgcaat
     2401 gcctgcagaa accctaaggc tcatgttcac actctttcag actatgattc ctttttctga
     2461 agctttttta cgagaaactg tgaatagccg tttgatgaaa aaattgaata gcgagctcgg
     2521 ggatcagcct tttgggacca tagatgctct tcgtgtggtt gatttatgta atgctcttgg
     2581 cggttattat cgtgacagat ttcccaatgg tgttgccatt atttcagata aaaccggaac
     2641 tcttacaact acaagaatgg atgtattagg gttatggact aatgatatgg ctcctgaggt
     2701 tcagaagaca ttaaaggaaa aagagggttt gctcctgcca gaagaaacac aatggctgca
     2761 aacttttgaa gtgttttgca atgcgtttac taacaataaa aaagaattgg aacctgagga
     2821 acatgctatt ttggagttat ttcaatcctt actcgataat cggcagtgtc tggaagtgat
     2881 tactttaggg aataatcatt ttaaaaaaga aatgatctct aaagacatta agaaagaaat
     2941 tgaaacattt catttgggtt tatatcgtac gtttggcggg cgctttactt tggttcagga
     3001 tggttctgat cattatctgg tcttttgtgg agtgcccaaa caagaggctt ttaaggagac
     3061 tcctttgcta aaagcctata cgactatgca aagtcgtact ggagtgttat ctcgcgattg
     3121 gtgtttagct cgaggtaaac taagtgaacg cgagtttact attttgaaag aattatttag
     3181 caaggatgat aaaaagcaga ttgagcattt tattttgcaa aataaattat tgctcgaaaa
     3241 tttgatgtat tacggtactt ttattattga taatcctgta aaaaaaggag cagaaaaatt
     3301 tattgcccaa tgtcgggaag tatcagttcc agtttttgta gcaacaggag acacgaccaa
     3361 ggcagcagaa aatattgcta aagtattgtg cccggctaat gcaggaaaaa ttgttacgct
     3421 gcgtgccgat cagattgatg aaaaaggtgt tgaattttta aatgaagaaa attatccctc
     3481 tgactctaca gttatatttt caggtattaa tgaagcgata ttgaacagat ttcaaaaact
     3541 gatggataga gaaggggcaa aacgaccagt cattattttt gctgaaatga gtactgaggg
     3601 aaaaggtatt ttagctcgtt atcttaagga ccatcactat tttgttgttg ccaacgggga
     3661 tggtaccaat gatgttatga tgatgaaaaa ttccaatgtg gtgatagcgc atcattcaga
     3721 tgatggaact tttgctccag gagtggatgg cttaaccaat attagtgagg agcagctaag
     3781 acggttgttt ggttcccaga aaacttttta tgaactgttt gacattaatc tacccaaaag
     3841 cctgttcgtt caaaaatttg ctcctctagc caactcacaa gaaaagccaa ccatggcctt
     3901 ggcactcaaa agtggaaaaa tgacgtttga tttggctaaa acagttggag cagatgtcac
     3961 agaaatgaat cagcagcatt ggtatagcgt tgtttttgac ttattatggt tgtggattgc
     4021 tttttatgaa ataaatgaaa gctccgattt gccaatggac aatcggaaca tcaatgcttc
     4081 aagattgatt tccaatacaa tggggattgc cttgataatc gctgtttttc aatcccttgc
     4141 caattatgct ctgtttaatg aatctacgaa cttgaccagt atgctaatta tgttgagttt
     4201 tttgcctcta atactaaaga gtattttctc aggttttaga atggttcagg acagcttgta
     4261 ccctcaggtg gaagtaactg aagttgagga agaaggagac acaagtacta atggatctgt
     4321 cctaagctat tttggaactt ttttttcacg caagccagta aagcaattag agggtttaaa
     4381 taatgctccc gcattaaaat aactctcttg cataagcggt ctcttttact ttattactgt
     4441 attattgcga tttcgcagtg ctcattctac aagcactctt atgcttattc acatatccca
     4501 gtaagcaatt aagtagagag gatacatgag aagcatctat aaacaattta ttaaatttat
     4561 aagaaaatat attgtcaatt tattatggta gagattaaat aactagatgc cgttattatc
     4621 atcggtcaat gataaggcgt taaaaaagga ttcataaaaa tatgcatgat gcccttccac
     4681 taattaccac tattgctatg ggatttgctt tagcattaat tttgggattt attgctgtac
     4741 gcttgaagat gccagcattg gtaggttatt tattagccgg cgttaccatt ggaccattta
     4801 caccaggatt aatggcaaat gtcgaaattg cccaggaact tgcagaaatt ggagttatgc
     4861 tgttaatgtt tggggtaggg ctacacttct ctttatccga tttgctgcga gttcgcaaaa
     4921 ttgcgatccc aggtgcgatt gtgcaaatga ttgtggcgac aatcctggga ggaggtactg
     4981 caatcatgtg gggttgggac tatggcagtg ctttaatttt tggtctttct ctttcagtag
     5041 ccagcaccgt agtactattg agggctcttg aagaaagagg tactctggag tcgattaatg
     5101 gccatattgc tgtaggttgg cttttggttg aagacctggc catggtgatt gtgcttgtat
     5161 taattccacc attatcacac tggcttggag ataattcatc agtgactata cacagaccgc
     5221 tatggattgt tctcggtctt accctcataa aggtaacaac tttcattgcg ctgatgtttc
     5281 tggtagggcg tcgatttttc ccaaagctgc tctggcagat tgcgcgtacg ggatcgcgtg
     5341 aactattcac tttatgcatt attacggcag ctattagtat tgcttatgtg gcctctaaat
     5401 tgtttggtgt ttcttttgct ttaggggctt tttttgctgg catgattatg cgggaatcct
     5461 cttttagcca cagagccgcc gaagagtctt tgcctcttag agatgcgttt gctgtgttat
     5521 tttttgtttc tgttgggatg ctatttaatc ctgcagtttt ggttgaacaa ccactacaag
     5581 ttttaacggt tgtcagtatt attattattg gtaaatcaat cgctgctttt atattggttc
     5641 ttgcatttcg ttatccaatt caatcagcac tgataatatc agcgagtcta gcccaaattg
     5701 gtgagttttc ttttatactt gcagagctgg gcatacgatt tgatatgctt cctgaggcgg
     5761 gaagaagtta tattttggcg ggtgcattaa tctcaatagc tattaatcct cttatcttta
     5821 aactaataaa tcctgttcat gcctgggttc aatcccaccc taaattagtt ggaattcttg
     5881 aacgaacaga tgatccttta gctgagctac ccaggacaac taaaaaagaa tatctttctg
     5941 ggcaagttgt tttggtaggt tatggacgtg taggcatgca tattgctcaa ttactttcta
     6001 aaaaagtagt tccttttgtt gtcattgatg aaaatcgtga gttaattgaa cgtttgcgag
     6061 cttcaggtat tcccgcagtt tatggagatg catctgagcc ttctgtacta attcaaggcc
     6121 atattgctcg tgcaagcatg ctcatgactg taacatcgga tactattcat gtaagaaaaa
     6181 tgattcaatg tgcgtataag cttaatccag acatagagat tgttgtacgt acacataacg
     6241 aggaagaagc agagctatta cagaaggaaa tcacagggaa agtatttttt gctgagggag
     6301 aaatagcaaa aagtatgggg cattatgctt tgaatcggta tggaaaatcg ccatgaagtg
     6361 tgagtcaaat ggaaagtttc cttcaaaatc aagatgattc ctgttgatca ttataaagca
     6421 ctcgcaattg agcaaagcaa ttttgaagtt ccaataagaa tcagattgca tataattttg
     6481 ctcaaatttt caagataaac taattgaata tgactaatgc ctacgggaat cgtattaagt
     6541 tcacaatccc ctgaagccac aacttctttt cctcgtcgag gaggaactgc tgctggaata
     6601 aaccatgtga ctggttttcc tattattaaa atgcatagag acgctcaggc tcgtcctgac
     6661 gagcgaaagc actttaatca aattaaagat ggcagcttga ttttcattac cggacatagc
     6721 aatactgctc tggatcatct tactggtgat tatgttcccc cattagcgac tccggttcag
     6781 tgaatgagaa tgatactgaa aataaatatg tcttcattgc aacatttttt caagccactg
     6841 atggtatcca tagcattcga tatggaataa acaaaaatgg agagcttttt tcaataaata
     6901 cgaatggcaa taaagttacg ccaattgata ttttgccaca gggtgtaatg gctgctttgg
     6961 cgcaatatat tactcaaaat aaagaattga taaagaatcg ctccataaaa aaggagctca
     7021 aatgcccctc agtccaagat tacaacatag atttaaacgt aaaaaagcag ttcgtactga
     7081 atagattcat ttaaaaggca agaaggagtt gccattaaaa cctgttcaaa cagatattct
     7141 ctcaaccaat gtacctgctt ctccaagaca gtcaatccaa gaaaatcagg gttgtttaat
     7201 gtttgttaca ccggtgcaag tacaggagtt gcttaaacct cgtggaaaaa cgatgaggaa
     7261 taaatatgag agaaaatgaa gaactaaaaa tggatttgag taaagggcaa cgaatacctg
     7321 tgatacagaa aaaacaacct cagaaacgta gacatcaatt ctttagtgat cccccccaaa
     7381 agtcagatga gcaaaggata aaactacgtc gcactcgcga aatctaacca aaggggctta
     7441 gctggtcagc atagtcttgt tggatgttca tcaaaactac gttacaacaa gccacaagtt
     7501 agcagcagat atgttataca tattttctaa cttaaatagg cagctagtgg gtactttttt
     7561 atcttaaaat gtagtagttc aaacttgatc tgacagttgc cggtttttca gaagtgtctg
     7621 tcaggtcaaa ttcagtttat aaatttttcc atccagattt gtttgctatc catcaaagtt
     7681 tgtatcggag tgcgtccaca acacattttg ccttgatggg ttcgctcatt attatagtaa
     7741 tgaagccatt cgtccagatc tttttgaagc tcatccatgt tatc
//
LOCUS       DATNSN010000150         7781 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.150, whole genome shotgun sequence.
ACCESSION   DATNSN010000150 DATNSN010000000
VERSION     DATNSN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.150"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..222)
                     /locus_tag="VAH76_002562"
     CDS             complement(<1..222)
                     /locus_tag="VAH76_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387158.1"
                     /translation="MYLMREQENVWESGVAKEKNKLKPIDAPIYSYWQALYMSFYSKR
                     LFVDVGKHWRGIGLIYLLLVIAICSIPFSI"
     gene            complement(173..1264)
                     /gene="ugpC"
                     /locus_tag="VAH76_002563"
     CDS             complement(173..1264)
                     /gene="ugpC"
                     /locus_tag="VAH76_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO1387159.1"
                     /translation="MATLNLIEVSKQVGETTILNRISIEIKKGEFMVVVGPSGCGKST
                     LLRLIAGLDVLSSGKILINNQCVNDTEAAKRDMAMVFQNYALYPHMTVFDNMAYGLKL
                     RRFSKADINKRVTAVANLLHLTPYLDRKPQALSGGQKQRVAMGRAIVRSPAVYLFDEP
                     LSNLDAKLRTEMRHEIKRLHQQFNTTSLYVTHDQTEAMTMADRILVLNQGEVEQIGTP
                     QELYQSPATQFVAGFTGHYPINFISGKYDKKTDSIYTDIGITYSAPNWLHSVDDKAEL
                     IVAIRPEHIQLCSSQSQDAVELYVEFVDDMGADKLIQAKCVKNNEQINLRISAEQMVS
                     NRQLHVELPKLKLHVFDERTGKRVGEWRG"
     gene            complement(1264..2091)
                     /gene="ugpE"
                     /locus_tag="VAH76_002564"
     CDS             complement(1264..2091)
                     /gene="ugpE"
                     /locus_tag="VAH76_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEO1387160.1"
                     /translation="MRQFKRFGSHGLILFFIGLMMAPLYLAVVAASHEGTVMMHSQLP
                     LIPGTSFFKNIKSVLTEGLAVTGGEPITSMLVNSLFMAFIIAVGKIILALGSAFALVY
                     FDFPFKKSCFALIFATMMLPVEVRIVPTFQVVASFGLLNSFSGLTLPLLASATGTFLF
                     RQFFKTISKELVDAAKLDGAGPLRFFIDIVLPLSRVQISALFVILFVYGWNQYLWPLV
                     ITTETKMTTIVMGIRYLAGVADQVPEWHYIMAVALIALVPPCMVVIVMQRWFEKGLK"
     gene            complement(2088..2966)
                     /locus_tag="VAH76_002565"
     CDS             complement(2088..2966)
                     /locus_tag="VAH76_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO1387161.1"
                     /translation="MSKFNHQRLFAWLFIVPQLIVTIIFFIWPACSALLQSFYYTDAF
                     GLHKNFAGLTNFFDLFLDPGYAKAIWVTFVIAISVTLITMSLGLLMATLINYRGKSQG
                     VYKTLLIWPYAIAPAVAAILWRFLCHPTLGWLTQVLEYLGIHFNYVNDANQALLVVIL
                     TASWQQFSYNFLFYFAALKAVPQTLVDAAIMDGANAWQRFWQIIFPILSPTTFFLLIM
                     NLIYGFFDTFGIIQVMTHGGPGNSTTNLIYKVYEDGFEGMDLGSSSAQSVLLMIIVIA
                     VSLLQFKYLEKKVHYE"
     gene            3086..4093
                     /locus_tag="VAH76_002566"
     CDS             3086..4093
                     /locus_tag="VAH76_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEO1387162.1"
                     /translation="MHSYLKKDLILHYIQINNPGPHSELTLAEGPNPGFNESQILVRV
                     KATALNRADLMQKYGKYPPPPGESDIPGLEVAGEVIVTGSKVKQFKPGDKIYGLVGSG
                     GYAEICPVEASLAHHIPSHWNYSLAAALPEALTTVYATLYDLGALSAGQTLLIHGAGS
                     GIASLAIQMAKLTAAKVITTVGDSSKIEKALALGADQIIHYKEQDFELLIEDNSVDLI
                     IDFVGGDYFNKHLHLLKPQGKLIQIACLKGSKVECSLALLMQKRLHIIGFVLRSQSIK
                     EKARLWKLAHDHWFNAFENDKIKPVIDSEFDLSQIETALQRMQQGSHFGKIVIRVGKQ
                     C"
     gene            complement(4108..4254)
                     /locus_tag="VAH76_002567"
     CDS             complement(4108..4254)
                     /locus_tag="VAH76_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387163.1"
                     /translation="MSQYVLIVQLWGIRIKVRRALDLSTKNTNSLDIAKAALEESVRI
                     ILSS"
     gene            complement(4261..5544)
                     /gene="clcA"
                     /locus_tag="VAH76_002568"
     CDS             complement(4261..5544)
                     /gene="clcA"
                     /locus_tag="VAH76_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HEO1387164.1"
                     /translation="MGKKILILYFVSVLLGILTGAIASLFQLTIQQMDHLLELLFYMT
                     ESYGLPVGIVSAMTSMVMLFIAWMMVKYIAPEAAGSGVQEIEGTLLHVRPIFWKRLIP
                     VKFFGGVLSISAKMVMGREGPTIQMGGNLGQMLGELFRLTRRRRDTLIAAGSAAGLAA
                     AFNAPLAGVLFVMEEMRNQFNYSFTNFKMVVICCVMATIVLHLIIGSQPAIQMEVFEL
                     PTLGSLWLFFVFGILMGLAGLLFNVTLMRTVGWLDKQNPKNKKYYILAVGFLVGYLAY
                     SYPATVGGGYVIISEALTMSPAFSVLCSLIVIRFIMTMLSYSSGVPGGIFAPMLALGT
                     LFGLGFFHLFQWLSMDFSIEPGMFAVAGMGALFAAAVRSPITGVVLVVEMTKNYSLIL
                     PLMVSCITATIVVQLARNEPIYTQLLERTLRLNAK"
     gene            complement(5537..7684)
                     /locus_tag="VAH76_002569"
     CDS             complement(5537..7684)
                     /locus_tag="VAH76_002569"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEO1387165.1"
                     /translation="MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASS
                     FEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQRGEILLSFLYPLLKTTDLWEVIS
                     QTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
                     YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREG
                     KYSPYQPIHKPEKNCDHEPGEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSIL
                     FRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIED
                     AHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHV
                     EGILRDGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGN
                     LNTGLVLRTVRIEKGTAEIRVGATLLYDSIPEAEEEETRLKASAFLDILQKPWQKAKK
                     KTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQNNHFDLI
                     VLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSS
                     VIRVLDNKGLFSDLGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPV
                     HAVQFHPETILSLSDQVGLRIIINLMNMVKDNG"
BASE COUNT         2552 a         1663 c         1304 g         2262 t
ORIGIN      
        1 tatagaaaac ggtattgaac aaatagcaat aaccaataat agataaatca aaccaattcc
       61 acgccaatgt tttcctacat caacaaaaag cctctttgaa taaaaagaca tatatagtgc
      121 ttgccaataa ctatagatag gagcatcaat tggttttaat ttattttttt ccttagccac
      181 gccactctcc cacacgtttt cctgttctct catcaaatac atgcaatttc aatttgggta
      241 gctctacatg taattgcctg tttgagacca tttgctctgc tgaaatcctc aagttaatct
      301 gttcattatt ttttacacat ttcgcctgaa tcaatttatc agcccccata tcatcaacaa
      361 attctacata aagttcaact gcatcttggc tctgactgga acatagttga atgtgttcgg
      421 ggcgaattgc tacaataagt tctgctttgt catcgacaga atgaagccaa ttaggcgctg
      481 aataagttat acctatgtcg gtataaatac tatccgtttt cttatcgtat ttgccagaaa
      541 taaaattaat aggatagtgc ccggtaaaac cagctacaaa ctgcgtagct ggactttgat
      601 acagttcttg tggcgttcca atttgctcaa cttccccctg attaagcacc agaattctat
      661 cagccatagt catcgcttcg gtttgatcat gagtaacgta taaactggtc gtattaaatt
      721 gttgatgcaa tcgtttaatt tcatgtcgca tttcagttct taattttgca tccagatttg
      781 ataatggttc atcaaataaa taaactgctg gagaccgcac aatagccctg cccattgcta
      841 ctctctgctt ttgcccacca gacagtgctt gcggcttcct gtccagataa ggagtcaaat
      901 gcaataaatt agctactgca gtcactcttt tattaatatc tgctttactg aagcgcctca
      961 actttaaacc ataagccata ttatcaaaga ccgtcatatg aggataaagt gcataatttt
     1021 gaaacaccat cgccatatcc ctttttgcag cctctgtatc attaacacat tgattattaa
     1081 taagtatttt tcctgaactt aaaacatcaa gtcctgctat caatcgtaat aaagtagatt
     1141 tgccacaacc tgatgggcca acaacaacca tgaattcgcc cttctttatt tcaatgctaa
     1201 ttcgattcaa aatggttgtt tctccgacct gttttgacac ctcgatcaaa ttcagtgttg
     1261 ccattatttc aatccctttt cgaaccaacg ttgcattact ataaccacca tacagggtgg
     1321 cacaagagct atcaatgcga cagccataat atagtgccac tccggcactt gatccgctac
     1381 acctgccaaa taccgaatcc ccatcacaat agttgtcatt ttagtttctg tcgtgatgac
     1441 caaaggccat aagtattgat tccacccata gacaaacaag attacaaaca aggcggaaat
     1501 ttgaactctt gacaggggta acacgatatc aataaaaaat cttaatggac ctgcgccatc
     1561 taatttagca gcatcaacca gttctttaga tatggtttta aaaaattggc gaaataaaaa
     1621 agttccagta gctgatgcca ataatggcaa ggttagacca gaaaatgagt tcaataggcc
     1681 aaatgaagca accacttgaa aagtggggac tattcttact tccactggta acatcatcgt
     1741 tgcaaatatc agggcaaaac aagacttttt aaaaggaaaa tcaaaatata ccaatgcaaa
     1801 agcagatccc agagccaaaa tgatcttacc cactgcaatg ataaatgcca tgaataagct
     1861 atttacaagc atggaagtga taggctcacc accagtcaca gctaaacctt ctgtcagcac
     1921 acttttaata tttttgaaaa atgaggttcc tggaatcaat ggcaactgag aatgcatcat
     1981 gacggttcct tcatgacttg cagcaactac agccaaatac aaaggagcca tcattaatcc
     2041 tataaaaaac aatattaacc catgcgaccc aaaacgttta aattgtctca ttcgtaatga
     2101 acctttttct caaggtattt aaattgtaat aaggaaactg caattacaat aatcatcaat
     2161 aatacagatt gggctgatga acttcccaaa tccattccct caaaaccgtc ttcatacacc
     2221 ttatagatta aattagttgt gctattacca ggacctccgt gtgtcatgac ttgaataatt
     2281 ccaaatgtat caaaaaaacc ataaatcagg ttcattatta acagaaaaaa ggtagtgggc
     2341 gacaggattg ggaaaattat ttgccagaat cgttgccatg cattggcccc atccatgatt
     2401 gctgcatcaa ctaatgtctg aggaactgct ttaagcgcag caaaataaaa taaaaaattg
     2461 taactaaact gctgccaact ggcggttaaa ataacaacca gcaaagcttg attcgcgtcg
     2521 ttgacataat taaaatgaat tcccaaatac tcaagtacct gagtcaacca tcctaaagtt
     2581 ggatggcata aaaaccgcca aagtattgca gcaacagcag gggctattgc ataaggccat
     2641 atcaataaag tcttataaac tccttggctt tttcctctgt aatttattaa agtagccatt
     2701 aaaagaccta aactcatagt gataagcgtc acactaatcg caattacaaa agtaacccat
     2761 atggcttttg cataaccggg atcaaggaat aagtcaaaaa aattggtcaa tccggcaaaa
     2821 ttcttatgca aaccaaatgc gtcagtataa taaaaggatt gcaataatgc gctgcaagca
     2881 ggccaaataa aaaaaataat agtgacaatt agctgaggga ctataaatag ccaggcaaac
     2941 aaacgctgat gattaaattt agacattaac taaccatgta gttgttaaaa tgataattat
     3001 catcgcaata gtttaaaata aaattatatc gattttaaat cattatgcga gttgtaaaag
     3061 atataatata gtaaattatc atactatgca ttcttacctt aaaaaggatc ttatcttgca
     3121 ttacatacag attaataatc caggacctca tagtgaatta acccttgccg aaggtccaaa
     3181 tcctggtttt aatgaatctc aaattcttgt gcgtgtcaaa gccacagcct tgaatagagc
     3241 tgacttgatg caaaaatatg gtaaatatcc ccctcctcca ggagaatccg atatacctgg
     3301 actagaagta gctggggaag taatcgtcac tggttccaag gtaaaacaat ttaaaccggg
     3361 tgataaaatt tacggtcttg tcgggagtgg aggttatgct gaaatctgtc cagtagaagc
     3421 ttctttagct catcatattc cttctcattg gaattattca ttggccgcgg cgctcccgga
     3481 agcgttaacg acagtttatg ctacgttgta cgatttaggt gccttaagtg ccggacaaac
     3541 attattgatc catggcgctg gaagcggaat agcctcatta gccatacaaa tggctaaatt
     3601 aacagcggct aaagtgataa cgacagtcgg tgattccagc aaaatagaaa aggctcttgc
     3661 cctgggcgct gatcaaatca ttcattacaa agaacaggat tttgaattat taattgagga
     3721 caattctgtt gatttgatca tcgattttgt cggtggtgat tattttaaca aacacttaca
     3781 tttattgaag cctcagggta aattaatcca aattgcttgc ctcaaaggaa gcaaagtaga
     3841 atgcagtctg gcgttgctta tgcaaaaaag gttgcacatc attggatttg tactcagatc
     3901 gcaaagcatc aaagaaaaag caaggctttg gaaattggct catgatcatt ggtttaatgc
     3961 atttgaaaat gacaagatta aaccagtaat tgactcggaa tttgatctaa gtcaaataga
     4021 aacagcactg caacgcatgc aacaaggctc tcatttcggg aaaatagtca ttcgcgtagg
     4081 taagcaatgc taagcctcat caatttctca tgagctcaat attatacgca cagactcttc
     4141 caaagctgcc ttggcaatgt ccagtgagtt cgtgtttttg gtagataaat caagggctct
     4201 tcttactttt atccttattc cccatagctg aactatcaat acatattgag acatttagac
     4261 tcattttgca ttgagtcgta aagttcgctc taataattga gtgtaaatcg gctcgttacg
     4321 tgctaattgc accacaatcg ttgcagtaat gcaggaaacc ataagaggta gaattagaga
     4381 gtaattttta gtcatttcaa caaccaatac cacgccagta attggtgatc ttactgcagc
     4441 tgcgaaaagc gctcccatac ctgcgactgc aaacatacca ggctctattg aaaaatccat
     4501 acttaaccat tgaaataaat ggaaaaaacc taatccaaat agtgttccca aagccagcat
     4561 tggcgcaaag ataccaccag gcacgccact tgaataggat aacatagtca tgataaacct
     4621 gattacaata agactgcata acacgctaaa tgcaggtgac atagtcagtg cttcactgat
     4681 gataacataa ccgcccccaa cagtagcagg ataggagtac gccagataac cgactaaaaa
     4741 tccaacagct aaaatataat attttttgtt tttggggttt tgtttatcta accatccaac
     4801 agttctcatg agtgtgacat tgaataataa gccagccagc cccattaata ttccaaaaac
     4861 aaagaacagc cacaaagagc ccagtgtagg tagttcaaaa acctccattt gaatagccgg
     4921 ttgtgagcca ataataagat gcaaaacaat cgttgccata acacaacaaa taacgaccat
     4981 tttaaaattg gtaaatgaat aattaaactg gttacgcatt tcctccatga caaacagtac
     5041 accagccaga ggcgcattaa atgctgcagc caaaccagcg gcagaaccag cggctattaa
     5101 agtatcacgg cgacggcgtg taagccgaaa caactctcca agcatctgtc caaggttacc
     5161 acccatctga atagtaggcc cttctcttcc cataaccatt tttgccgata tggataacac
     5221 acccccaaaa aattttactg gtatcagacg cttccaaaat attggtctca catgaagcaa
     5281 ggtaccttca atttcttgca caccacttcc tgccgcttct ggggcgatgt acttcaccat
     5341 catccaggca ataaataaca tcaccatcga tgtcatagcg gaaacaattc ctacaggaag
     5401 tccataactt tctgtcatat aaaataatag ctccagcaga tgatccatct gttgaatagt
     5461 aagttggaat aatgaagcaa ttgcgcctgt taatatccct aatagaactg atacaaaata
     5521 taaaatcagt atttttttac ccattgtcct tcaccatgtt catcaaattg atgattattc
     5581 ttaaacccac ttgatccgat aaagacaaaa ttgtctctgg atgaaattga accgcatgca
     5641 caggtaaata ctcatgagaa actgccataa tcacatcatc ttcacttaca gcggtaacta
     5701 ataattcatt gggtaaagcc tctgctctgg catagagtga atggtatctt cccgctttaa
     5761 aacaatcacc taaatcggag aacaacccct tgttatctaa taccctgatt actgaagatt
     5821 ttccatgcat agggtattca agaacatcca aaacccctcc aaaatattca acaattcctt
     5881 gcagccccag acaaacaccg aatattggta tgttctttga gagtatcaca tcgattgttt
     5941 ctgaaagctt aaaatcaacc ggttttccgg ggccaggcga taatacgatc aaatcaaaat
     6001 gattattttg taaatagcca atcgccttgt catagcgaac tgttgtaacc gcagctcccg
     6061 tttgtcttaa atagtttgcc aaagtatgaa caaaggaatc ttgatgatcg ataagtaaaa
     6121 cttttttatt tttgcctacc aggggaacat cttctgtctt ttttttagcc ttttgccatg
     6181 gtttttgtaa aatgtctaaa aaagcagaag cttttaatct cgtctcctct tcctcagctt
     6241 cagggataga gtcataaagt aacgttgcgc ctactctaat ttctgcagtt cccttttcta
     6301 ttcgcactgt tcttaagacc aatccagtat ttaaatttcc atcaaaacca aaccatccta
     6361 cagcccctgc ataccactta cgtggggatt tctcatgttg ttcaatgaaa ttcatagccc
     6421 aaattttagg cgcaccagtt acagtaacaa cccacatatg agttaaaaaa gcatctactg
     6481 catcaaatcc atctcttaaa ataccctcaa catgatcaac tgtatgaatc aatcgggaat
     6541 acatttcgat ttggcgacgc ccaatgactt tcacactccc tgcttcgcat attcttgact
     6601 tatcatttct atccacatcg gtgcacatgg ttagttctga ttcttctttc tctgaatcta
     6661 gaagtgtttg aatattgtga gcatcctcta tagcatctgc tccccgtttg atagtgcctg
     6721 agatagggca agtttctact cttttacctt ggacacgaac atacatttca ggtgaagcac
     6781 ctaccagata ttcttcgtca cccaaattaa caaaaaatcc atatggcgat ggatttattt
     6841 ctcgcatttg gcgaaataat attgacggct tttcagcaaa gtgagtatag aaagtttggc
     6901 taggaaccac ttcaaataaa tcaccacagg aaaatttttc tttggctttt ttgaccactt
     6961 cggcgtattc acctggctcg tgatcacagt ttttttccgg tttatggata ggttgatatg
     7021 gtgaatactt cccttctctg gataaagatt gagttgatcg cccctgatat tgaaaatcat
     7081 agcgtctaac aaaggcttct tccttacgat gattcactat ataaatttca tcaggtagat
     7141 agagaaccat atctctctgt gtttcatttc ttggtttaca ccgctccaac tgttcaaatt
     7201 gataaatcag gtcaaaccca aaagcaccat ataaacccaa gtatgaatct tcttctgttt
     7261 taaaaaaatt caataagagg cgaattgcac tgaatactga cggttgatgg ctccgctcct
     7321 cctcactaaa tactttgtct gatgatttaa tttttaactg acaaaatgag gtcgtttggc
     7381 taattacctc ccagagatct gttgttttta acaacggata aagaaaagat aataatatct
     7441 cgccccgttg atttaatgcc tcgaaacata tggtattatt ttgacagatc ataactaatg
     7501 ggggattata aaaacccatg tcccaacaag tatagcgtcc cggatattca aagctcgaag
     7561 caaatactgc tccacgttga gaatcaagac tctcaagcaa aaaatctatg ccttgttgat
     7621 aatccaactc ttgttgagaa aactcaacat gaacaccacc gagagtttta tattgttgta
     7681 acatgaatac cattctctat catttaattc gtacaaatat tcattctact gtaaaaccac
     7741 tgttctgcaa gaatcttgta acatttcaat atcattgctt t
//
LOCUS       DATNSN010000151         7658 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.151, whole genome shotgun sequence.
ACCESSION   DATNSN010000151 DATNSN010000000
VERSION     DATNSN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.151"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            131..1654
                     /gene="murA"
                     /locus_tag="VAH76_002570"
     CDS             131..1654
                     /gene="murA"
                     /locus_tag="VAH76_002570"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO1387166.1"
                     /translation="MLSKYELIKPDIGSSHGNITKVYEIVGGKPLRGQVKVSGSKNAA
                     LPVLAASLLCKEPLQLTNLPKILDVTNMIQCMEALGKRFEFINTNTIKIGNREMDTLT
                     IPKEAARQIRASIILLGPLVAQYPKVILPLPGGCSIGKRPIDLHISALNALGVDIVEL
                     PDCLVCSRRYARLQGNTINFSIKTVTGTENAMMAAVLAEGTTIINNAAMEPEIVDLAN
                     FLNRLGAKIDGAGSDRITISGVDKLNSCGSYSIMADRIEAGTYLVAAAMTRGSISVKG
                     INPDYLVAVLSELKCAGAKITCDSDSIQLDMGEEYGESLNITTKPYPGFPTDLQSLFL
                     SLTCVLRGNSYLCETLFEDRFQIVQELKKMGANISLKDNTAHIKGVSRLRGSQVNATD
                     LRSGAALVCASLAAEGITQIFAIEHIERGYEDLILKLKALGANIKLIENTHVTSTELS
                     DSHFKEATSSETRIQRASLIETVVTPSITTFGQFKRVPTGEVTELEKQQTPYLHHIM"
     gene            complement(1647..2180)
                     /locus_tag="VAH76_002571"
     CDS             complement(1647..2180)
                     /locus_tag="VAH76_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEO1387167.1"
                     /translation="MNYSIRKFEDKYPELGERVYIDPQSTVIGNVTLGDDVSVWPMAV
                     IRGDVNYIQIGHSCSIQDGAVLHVTHDGPYTSGGRPLILGQGITVGHKALLHACTIDD
                     YCLIGMGSIILDSAHIQKHVMIAAGSIVPPGKILKSGHLYLGSPAQAIRKLTTKEIEQ
                     IEYSAGHYIRLKDRYLT"
     gene            complement(2187..3545)
                     /gene="gorA"
                     /locus_tag="VAH76_002572"
     CDS             complement(2187..3545)
                     /gene="gorA"
                     /locus_tag="VAH76_002572"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO1387168.1"
                     /translation="MKTKHFDLIVLGGGSGGIASAVRAAQYGAKVAVIEQSHLGGTCV
                     NLGCVPKKIMYNASSIAETLHKSPDYGFFLENNAKLDWKRLVNKRNAYIERLRENYAK
                     RFSQHKITLIQGKGIFHDQSSITIDHTIYQAEHIIIATGGEPALPAINGIKHAIDSDG
                     FFSLTKLPAKVAVIGSGYIGVELAGILNSLGSETHLLMRGTRPLSRFDHMIGDTLMEI
                     MQKQGICIHQNHKAQAIHLHSDGRKSILCQSGSIIENIDVIISAVGRKPRTGNLNLDK
                     INVNMDDKGLILVDAFQNTSVKGIYAIGDVTNAPALTPVAIAAGRRLADRIFGNQPDA
                     CLNYDNICSVVFSHPPSGSVGLTEHEAIEKYGKNKIKIYQTRFIPMYDALSIDKTPTA
                     MKLVTLGKKEKIIGLHVVGYSADEMLQGFGVAIKMGACKKDFDNTIAIHPTSAEEFVT
                     MV"
     gene            complement(3776..4756)
                     /locus_tag="VAH76_002573"
     CDS             complement(3776..4756)
                     /locus_tag="VAH76_002573"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO1387169.1"
                     /translation="MNLKKAELHVHLEGTISPDLAKKLAKRNHLTLPDGLIAFDEKSY
                     LSKDFLDFLKVYDTLASVIKNPQDYYDITFDYLKSNAQEHAIYIEMMYSPDHAEQSSG
                     IPSKEHLAAIQQAIDDAKSQFDIVGRIIITAVRHFGVEACERVAKQASIDKFPCVTGF
                     GLGGDEAKFPPQLFAKTYQIAADSGLECTVHAGEFDSAKGMEEAMKTLPIKRIGHGVR
                     VIDSPDIMAMVKDQGIALEVCPTSNIFLGLFKDMNSHPFPKLYEAGIKVSINSDDPPF
                     MSTTLAQEYKRVQKAYGYSNDTMNNITRMAIEAAFVDEKTRKELLTKLEQ"
     gene            4854..5039
                     /locus_tag="VAH76_002574"
     CDS             4854..5039
                     /locus_tag="VAH76_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387170.1"
                     /translation="MNTDDINKAYVSPYDKFLYEFDATHKKSASQLQEIKKHERIFKM
                     RDDKDYKIDQSEIWEEF"
     gene            5138..5584
                     /locus_tag="VAH76_002575"
     CDS             5138..5584
                     /locus_tag="VAH76_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387171.1"
                     /translation="MKNPYILQLNNPALLNKAMQLKSIGKVAISQNQLTYLKIHDEWI
                     YELFPLLQNEQIRKPDYFGNESIGAHISIIYPEENTTINPKELDQEHRFKIKNIIKAN
                     LELKKYYAIIVEAPSLARLRKAYGLPENLYFKGYSTHFHITVGVEY"
     gene            complement(5625..6878)
                     /locus_tag="VAH76_002576"
     CDS             complement(5625..6878)
                     /locus_tag="VAH76_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEO1387172.1"
                     /translation="MDYSIYLLFAALILCFLMTWGVGANDLANVMSTTMGSKAVTVRQ
                     AMLIAIIFEFAGAFLGGEGVTETMRDGIINTSQLSNEPLILIEGMLCVLFACTIWMNL
                     ASYLGVPVSITNALVGSMVGFGTIVLGPDAIHWNQVARIAIGWVSSPLISGITAYALF
                     ISIQQTIFVKSNPLTKAKLYIPIYLFLIGFILSFITVFKGLNHFDIHLNLKQDLAVTL
                     ATSIIITILGMIFIKRIPEYHKIRRRERFIQVEKYFAVLMAMTACAMAFAHGSNDVAL
                     AVGPLSIVHSLVMHSNQIFDADNYPSWIILLGCVGVVTGFLMYGRKVIETVGSSITAL
                     TPSRAFAATLSAATTVVVATSTGIPVSATQTLVGAVLGVGLARGIGALNLIVIRNIFM
                     SWVLTLPAASMLTILSYKLLHALLG"
     gene            complement(6888..7541)
                     /locus_tag="VAH76_002577"
     CDS             complement(6888..7541)
                     /locus_tag="VAH76_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HEO1387173.1"
                     /translation="MFGPSPIKPIEQHIRKAHQCAKQLYPFFEAVLKNDWDTANKIKD
                     KIIAIEKEADLIKRDLRLHLPTGLFLPVARTDILELLSAQDRIANKAEDIAALIISRQ
                     MSIPKKLIPSFMPFLSRCLDASKQACTAINELDELLETGFRGSEVKIVEEMIVKLDEI
                     EHDCDERLADIRHKIFELEKELPAIDVIFLYKLVQWIGELADHAQTVGGRLQILIAR"
BASE COUNT         2436 a         1436 c         1471 g         2315 t
ORIGIN      
        1 ccagtgcagg tcattcatgc atctcaaaaa gttatggttt ggaagtatag tgcttttata
       61 tgataaagca gtatcaataa gtaacagaca aaaagagcta taagtatttc aagattaaga
      121 tgggtggtta atgttaagca aatatgagct aattaaacct gacataggat ccagtcacgg
      181 caatatcaca aaagtgtatg aaatagtcgg cggcaaaccg ctcagagggc aagtcaaagt
      241 aagcgggtct aaaaatgctg ctctgcctgt actggcagcg agtttattat gcaaagagcc
      301 gctgcagtta accaatttac cgaaaatatt agatgttacc aatatgattc aatgcatgga
      361 agcattaggt aaaagatttg aattcatcaa tactaacacg attaaaattg gcaatagaga
      421 aatggataca ctgactattc caaaggaggc agccagacaa attcgtgctt caattatttt
      481 attaggccct ttggttgcgc agtatccaaa ggtgatattg ccattaccag gtggatgctc
      541 tattgggaaa cgtcccattg atttgcacat cagtgcgctt aatgctttgg gtgtagatat
      601 tgttgagcta ccagattgtt tagtatgttc tcgccgttat gctcggcttc aaggaaatac
      661 tattaacttt tccatcaaga ctgtgactgg aacagaaaat gcgatgatgg ctgctgtatt
      721 ggcagaagga acaactatta taaataatgc tgcaatggaa cccgagatag ttgatttggc
      781 taatttttta aataggcttg gagcaaaaat tgatggggcg ggtagcgaca gaatcacaat
      841 ttccggtgta gataaattaa attcttgtgg aagttattcc atcatggcag atagaatcga
      901 ggctggtacc tatttagtag cagcagcgat gactcgcggt tctatttccg ttaaaggaat
      961 caatccggat tatctggttg cagttttgag cgaattaaaa tgtgcaggtg caaaaattac
     1021 ctgtgattct gactcaatcc agcttgatat gggagaagag tatggtgaat cgttaaatat
     1081 aaccacaaaa ccctatccag gctttccaac agatctgcaa tctctctttt taagtctgac
     1141 ttgtgtttta aggggtaact cttatttgtg tgagactcta tttgaagatc gttttcaaat
     1201 agtccaagag cttaaaaaaa tgggagccaa tatttcttta aaggataaca ctgcccacat
     1261 aaagggtgtt tcaagattaa ggggctctca agtcaatgct actgatttac gctcaggtgc
     1321 cgctttagta tgcgccagtt tggcagcgga gggtatcacg cagatttttg ccatagagca
     1381 tatagagcgc ggttatgaag atttaatatt aaaattaaaa gcacttggcg ccaatattaa
     1441 attaattgag aatacccatg taacaagcac ggaattatct gacagtcatt tcaaagaggc
     1501 gactagtagt gaaactcgaa tacaacgagc atcgttgatt gaaaccgtag taacaccatc
     1561 tataacaaca tttggacagt ttaaaagagt acctacagga gaagtgacgg aattggaaaa
     1621 acagcagaca ccttatctgc atcacattat gtaaggtatc tatcctttaa tcttatataa
     1681 tgcccggcag aatattcaat ttgctctatc tcttttgtgg tcaattttct gatggcttga
     1741 gcagggcttc ccaaatacaa atgcccactt ttgagtattt ttcccggagg tacgatgctt
     1801 cctgcagcaa tcataacatg tttctgtatg tgcgctgagt ctaaaattat agatcccatg
     1861 ccaattaaac agtaatcatc gatagtgcag gcgtgtaata gagctttgtg ccctaccgtg
     1921 attccttgcc ctaaaattaa tggcctaccg cctgaagtat aaggaccatc atgagtaaca
     1981 tgtagaacag caccgtcttg aatactgcag gaatggccta tttgaatgta attaacatca
     2041 ccacggataa cagccatagg ccatacggat acgtcatctc ccaaggttac gttaccaatg
     2101 acagtggatt gcggatcaat ataaactcgt tcacctaatt caggatactt gtcttcaaat
     2161 ttacgaatgg aatagttcat gtatatttac accatagtta caaattcttc agcactggtt
     2221 ggatgaattg ctattgtatt atcgaagtcc tttttacatg cgcccatttt tatggcaacc
     2281 ccaaagcctt gtagcatttc atcggcgctg tatccgacga catgtaaacc aataatcttt
     2341 tcctttttac ccaaggtaac caatttcatc gcagtagggg ttttatcaat actcaaggca
     2401 tcatacatag ggatgaaacg ggtttgatat attttaattt tattcttacc atatttttca
     2461 atggcttcat gctcggttaa accgacagaa ccagatggag gatgagagaa tactactgaa
     2521 caaatattat cataatttaa gcaggcatca ggttgattcc cgaaaattcg gtctgccaaa
     2581 cgcctccctg ccgctattgc aacgggtgtt agtgcgggag cattagtgac atcgccaata
     2641 gcataaatac ctttaaccga tgtattttga aatgcatcaa ccaggattaa tcctttgtca
     2701 tccatgttaa cgtttatttt atcaagattt aaattgccag tccgcggttt tcgacctaca
     2761 gcagatataa tcacatcaat attttcaata atagagccac tttggcacaa aatacttttt
     2821 ctgccgtcgc tatgaagatg gatagcttgg gctttatggt tttgatgaat acagattcct
     2881 tgtttttgca taatttccat taaagtatcg ccaatcatat gatcaaagcg actcagtggc
     2941 cgtgtacctc tcattaataa gtgagtttca ctaccaagag agttaaggat tcctgctaat
     3001 tcaacaccta tgtaaccgct accaataaca gctactttag ctggtaattt ggttaatgaa
     3061 aagaaaccat cagaatcaat agcatgctta atgccattaa ttgctggtag agctggctca
     3121 ccaccagttg caataataat atgttctgct tgataaatag tatgatctat cgtgatagaa
     3181 ctttgatcgt ggaaaatacc cttaccttga attagagtaa ttttgtgctg tgaaaatcgt
     3241 ttcgcataat tttctcttaa acgttcaata tacgcattgc gtttattcac tagtcgcttc
     3301 caatctaact tggcattatt ttccaggaaa aaaccgtaat caggagattt atggagtgtt
     3361 tctgcaattg atgaagcatt gtacataatt tttttgggga cacatcctaa attcacgcag
     3421 gtccctccca agtggctttg ctcaataacc gctactttgg ctccatactg agcagcacga
     3481 acagcactgg ctattccgcc acttcctcct ccaagaacta taaggtcaaa atgttttgtt
     3541 ttcatagtca attaatagga aaataaaata atagtagcag gtcgtgcttg gtctaccaag
     3601 ggattaaact gaattttata aaaaactaaa atatttttta atttcaacca gatagtttta
     3661 gttatggttg gtgtttaagc catgtatcaa caccaacctg taaaaatata acagtatcat
     3721 tatcgcctca agtcagcagg gaattagtac tgacaacagg aatataagac tgagattatt
     3781 gttctaattt tgttaaaagt tccttccggg ttttttcatc aacaaaagca gcttcaatag
     3841 ccatacgcgt aatattattc attgtgtcat tgctatagcc ataagctttt tgaactcttt
     3901 tgtactcttg agcaagtgta gtgctcataa aaggaggatc atcggaatta atactgactt
     3961 taatccctgc ttcataaagc tttggaaaag ggtggctatt catgtctttg aataatccca
     4021 aaaatatgtt actggttggg cacacttcca atgcaatacc ctggtcttta accatggcca
     4081 tgatgtcagg tgagtcaatc actcgaaccc catgtccaat tcttttaata ggcagagttt
     4141 tcattgcttc ttccattcct ttggctgaat caaattcgcc ggcatgaacg gtgcattcca
     4201 atccggagtc tgctgctatc tgataagttt tggcaaacaa ttgaggagga aattttgctt
     4261 catctcctcc taatccaaat cctgttacac aaggaaattt atctatagaa gcttgttttg
     4321 ctacgcgttc gcatgcttct acgccaaaat ggcgtactgc agtgataata atacgtccaa
     4381 caatatcaaa ttgactttta gcatcatcaa tagcctgttg aattgctgct aaatgctctt
     4441 ttgaaggaat tccactggat tgctcggcat ggtccggaga gtacatcatt tcaatgtaga
     4501 tagcatgttc ttgagcgttt gatttaagat aatcaaaggt aatatcataa taatcttgtg
     4561 gatttttaat tacggatgcc agcgtatcat aaaccttaag aaaatcgaga aagtcttttg
     4621 ataagtaact tttctcatcg aaagcaataa gaccatcagg gagagttaaa tggtttcttt
     4681 ttgctaattt tttggccagg tctggggaaa tagttccttc taaatgtacg tgtaattctg
     4741 ccttttttaa attcatgata tagcctatta tcttacttga aagaagatta atggtaactt
     4801 agataaaaaa acgataaaat atatttttta aataaagtta ttttggattg agtatgaaca
     4861 ccgatgatat aaataaagct tatgtcagtc cttacgataa gtttttatat gaatttgatg
     4921 cgacacataa aaaatcagca tcgcaattgc aagaaattaa aaagcatgag cgtatattca
     4981 aaatgagaga cgataaagac tacaagatcg atcaaagtga aatttgggaa gagttttaag
     5041 agtttctcac ctatagctat cttggtaagc tgctagcatg ttaatcaaga cctagtagca
     5101 gaaatgacag gtctttattg tattagtaaa gataaagatg aaaaatccat acattctgca
     5161 actaaataat cctgctttat taaataaggc tatgcagctt aaatcaattg ggaaagtagc
     5221 catttctcaa aatcaattaa cttatcttaa gattcatgat gaatggatct acgaactatt
     5281 tccactctta caaaacgagc agatccgtaa acctgattat tttggtaatg aatccatcgg
     5341 tgctcatatt agtattatct atccggaaga aaatacaaca attaatccaa aagagttaga
     5401 tcaagagcat cgctttaaaa ttaagaatat tattaaggca aatttagaat taaaaaaata
     5461 ttatgccatt atagtggagg caccctcttt ggcaagattg agaaaagcat atggtttgcc
     5521 tgagaattta tattttaaag gatattcaac tcattttcat attacagtag gcgttgaata
     5581 ttaggcaatc cattgcttat gatgagcctg ataggcaatg aggattatcc caacaaagca
     5641 tgtaaaagtt tataggataa gatcgttagc atggaggctg caggtaatgt cagtacccat
     5701 gacataaaga tattacggat tacaatgaga ttcaaagcgc cgatacctct tgccaaacct
     5761 acacctaaaa cggcaccaac caaagtttga gtcgccgaaa cagggattcc ggtgctggtt
     5821 gcaacaacta cggtagttgc tgcagaaagt gttgcagcaa atgcacggct gggagttaaa
     5881 gcagtgatag aacttcctac ggtttcaatg acttttcttc catacataag gaaaccagtt
     5941 actactccga cgcagcctaa taaaataatc caggaagggt aattatctgc gtcaaatatt
     6001 tgattggaat gcatgaccag gctgtgcaca atacttaatg gtccaactgc caaggcaaca
     6061 tcgttggagc catgagcgaa agccatagcg catgccgtca ttgccatgag cacagcaaaa
     6121 tacttctcta cctgaataaa tcgttccctg cgtcttattt tatggtattc aggaatacgc
     6181 ttaataaaga tcatacccag aatagtaatt attatactgg tggccagagt caccgccaaa
     6241 tcttgcttta aattcaagtg aatatcaaaa tggttaagtc ctttaaatac agtgataaaa
     6301 gataatataa aaccaatcaa aaaaagataa ataggaatat atagcttggc tttagtcaac
     6361 gggttacttt ttacaaatat ggtttgctgt atactaatga acaatgcgta ggccgtaatc
     6421 cctgaaatca acggagatga aacccatcca atagcgattc gcgctacctg gttccaatgt
     6481 attgcgtcag gacccaagac tatagtgcca aaaccaacca tggagcctac taatgcgttg
     6541 gttatcgaaa cagggacccc cagataactc gcaagattca tccatattgt gcaggcaaac
     6601 aaaacgcata gcatgccttc gatgaggatt aaaggttcat tagaaagttg actggtattg
     6661 ataatcccat cacgcatggt ttcagtgaca ccttcgcccc ctaaaaaagc gcctgcaaat
     6721 tcgaaaatga tggcgataag catggcttga cggacggtaa ctgcctttga gcccatagtg
     6781 gtactcatga cattagccaa atcattggca ccgacccccc aggtcataag aaaacaaaga
     6841 ataagagcag caaaaagtag gtaaattgaa taatccatga gtgataacta ccgggcaatt
     6901 agaatttgaa gtcgaccacc aactgtctgg gcatggtcag ccaattcgcc aatccattgg
     6961 accagtttgt atagaaaaat tacatctata gctgggagtt ccttttctaa ttcgaagatt
     7021 ttgtgcctaa tgtctgccag tctttcatca cagtcatgtt caatttcgtc taattttact
     7081 atcatttctt caacgatttt gacttcgctg cctctgaatc cggtttcaag caattcatct
     7141 aattcattaa tcgctgtgca ggcttgttta gaggcatcaa gacatcggct taaaaaaggc
     7201 ataaaagaag gaattaattt tttaggtatg ctcatttgtc tgcttataat taaagcagct
     7261 atatcttctg ctttattggc aattctatct tgagcactga gcagttctaa tatgtctgtg
     7321 cgagcgactg gtaaaaataa gccagtaggt agatgaaggc gcaaatcacg tttaattaga
     7381 tccgcttctt tttcaatagc gatgatttta tccttgatct tgtttgcagt atcccaatca
     7441 tttttgagca ccgcttcaaa aaaagggtac aactgcttgg cacactgatg tgctttacgt
     7501 atgtgttgtt caatgggctt tattggtgat ggcccgaaca tattgaatat actacccata
     7561 atttttgatc tcggataaca gattttaagg attataccta aactttttga ggaacaaaac
     7621 tactacttat ctacagtgaa tacagattag ggattaaa
//
LOCUS       DATNSN010000152         7606 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.152, whole genome shotgun sequence.
ACCESSION   DATNSN010000152 DATNSN010000000
VERSION     DATNSN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.152"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            223..1266
                     /locus_tag="VAH76_002578"
     CDS             223..1266
                     /locus_tag="VAH76_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEO1387174.1"
                     /translation="MAYHQLSYTFLNHQKLPLIIQPQAKAEGNIDVLKQFLKEENALF
                     KEQLLNYGAIILRGFHVNTAEQFLEVIQSSDLGPNYSYDFCPIPRTQIRENIYTSINI
                     HPSFNLALHNEKSYDQDFPSHIFFNCIHAPQTGGDTALADGNKIWFSLPKFLQKKLQS
                     KGILYRRHYYGSGIQYKILRSIGKNSGCMTWMERFQTDDQNKVEVMLQKMGVQFRWIQ
                     GNGLIIEQLLPACRNHPISGKLVWFNQSNHANHYYNGTSDYIKSKINNTFSRFILLHK
                     YFHPYIAFYGDGEPLSKQEADCINSAIQKNTVSTAWQPGDVMIVDNFSCLHGKTPHTG
                     NRLILVGLTKYFD"
     gene            1281..3173
                     /locus_tag="VAH76_002579"
     CDS             1281..3173
                     /locus_tag="VAH76_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEO1387175.1"
                     /translation="MPDDISRKQFSIGDLYFTNELEVSGLIYEIFYQKSYLSDFLTLS
                     PGAVVFDVGANIGVFSLFVLKQCHYDTEIYSFEPVPATFKCLKKNLARFKHNVHVYNA
                     GIGNVPKDCSIDFTLFGESSVTATYKPTDKIISNYQPLLNYETLLKLSSFQNKPLYYQ
                     LKYLPFLRNYLIKKNYKHQTLETKVRCHLTALGRFIENNQITRIDLLKIDVEGAELDV
                     INSIKPEQFSLIKQLSIEVHDIDNRVEKLVSYLQKQGYITYVDRNPIFAELGFNHHMI
                     YAKLPEPAIITLSEEPNNPENYIEARQYFYGLLAGGVRIKLLESMFDLDLFRLFTGKP
                     YLLENDIIKRLELKPVRAKKWLHLLCCEDFLKKIMVGSQVGYQLAKSQLMLGEGYWGF
                     KQYYDFYWQRMANEKLSNILRFTDPKFNVSWPPKTAEEANFLETWMTKTATPLIQTLL
                     ACLDLNQYRSILDVGGGDGTIACALAQAYPHLKITVYNLPESAKIAQKNIDAMGLQKR
                     ISVFSGDFINDEQFPAGFDLILFVRVLWDWDNSRKRKLLNMAYHALKRKGHVAICEGF
                     KELCYDLCLTWEYSYIFADGFDAEVFKTSDEYKIMLQQIGFTPIPIKSISPSEPVPGT
                     VVLAEK"
     gene            3413..3694
                     /locus_tag="VAH76_002580"
     CDS             3413..3694
                     /locus_tag="VAH76_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1387176.1"
                     /translation="MAKQIKKLKTPNLQQPLTAELLGEVIKARRTQSNLRLEDAAALC
                     GVAKETFMKIEHGQSNCQLASVLQICSGLGISLYIKPWMDNSEDENDWR"
     gene            3681..4979
                     /locus_tag="VAH76_002581"
     CDS             3681..4979
                     /locus_tag="VAH76_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEO1387177.1"
                     /translation="MIGDKSVLDVYLGESQIANITLMDDQLYWSYHESWQQTGYAISP
                     HLPMHEDIPPLNVQRFLRNLLPEGNPLEVLVNVFHLSKYNTFGLIQALGLDTPGSLVI
                     LPSNQAIPKHANFRLVTHEELEERLNHRDSYNLIVWDGKPRLSVPGVQDKINVVLNKE
                     GQLGFGEGSLCSTHILKFETQKLSYLVLNEFLSMWLAKCCELNVANVQMKRFGQNPAL
                     LVERFDRKLITTTNIVKRRHLIDGCQAMNLPPEFKYEQNFGNSRDVAHIRDGVSLPKL
                     FDFANQCINPAKTKQQILDWVLFNVLIFNCDAHGKNISFFVGANGISLAPFYDLVNIK
                     MYPEFEHALAMALGDEFDGYNINAYQLADFADTCRLPRSLVAKHLKYLIGKLTTALQE
                     ETQFNLVGNEEDYFKKYQEIVMKRCEHLLKQNDQITSIKL"
     gene            5129..5743
                     /gene="lem15"
                     /locus_tag="VAH76_002582"
     CDS             5129..5743
                     /gene="lem15"
                     /locus_tag="VAH76_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem15"
                     /protein_id="HEO1387178.1"
                     /translation="MIYKITLTENNLAQAFMSIPQDVIALDLSNNLLGSKNCVDLLVN
                     IADCIPPGVTSLDLSSNGLRLTQVSLVTPKHVTSLNLCFNPLHEISIDGLEWLKDSLA
                     HIQIIYLSYDMVNNMSREQRQAFRAVIPNIQTIILVDKNGKEIGPSHTAKIANPIREL
                     GGGNPEVPSLLNQCTFFVKTQGINIDKAYIPEELKKRIHDCNPL"
     gene            6497..7240
                     /locus_tag="VAH76_002583"
     CDS             6497..7240
                     /locus_tag="VAH76_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387179.1"
                     /translation="MSRSKFFKSNRTHVIELYCYSDEYAQQVNHEITSGADSGPLLTK
                     IYGQDVRFIYAPDSEKFNLVLNEARKRNYNQPIINLYEPDNIKYLLSRLSHGDSILIN
                     GQGDIHKQLIAGRNAEELVDILENDLELKEISLKNLDIDSCRMGRVESYRHELKHHLK
                     NFQTVTTYTDLCTASQRGGVPYRMWIEERADRDVFYTESDLNKKGTRIIEYTDTYKNS
                     LKEIWKTNPYNLEEIDLSDHIDILVIASC"
BASE COUNT         2560 a         1221 c         1422 g         2403 t
ORIGIN      
        1 agtcctgttt gagtgattca gcaggagctt ctttggcaaa atctggtgga ttttcattgc
       61 atagcaaggt cagtgtctat actatttaat ataggtattc gtcaaagatg atgcggtaat
      121 ttttgtaata taggtgaaaa gagggagcca cttatagaat caggaaggac ctacactctg
      181 aaagtctgat agtacacctc cggattgtca gtgaggaatt ttatggcata tcatcaactc
      241 agctatactt ttttgaacca ccaaaaatta cctttaatta ttcagcctca agctaaagcg
      301 gaaggtaata ttgatgtttt aaaacaattt cttaaagaag aaaatgccct gtttaaggag
      361 caactcctca attatggcgc cattatctta cgtgggtttc atgttaatac ggctgaacaa
      421 tttttagaag tgatccagtc aagtgatttg gggccaaatt attcttatga tttttgcccg
      481 attccgcgaa cacaaattag agaaaatatt tatacttcga taaatatcca tccgagtttt
      541 aatcttgctt tacataatga aaaatcatat gaccaggatt ttcccagcca catttttttt
      601 aactgcattc atgccccgca aacaggcgga gatactgcat tggctgatgg aaacaagata
      661 tggttttctt taccaaagtt tttacaaaaa aaattgcaat ctaaaggaat attatatcga
      721 aggcattatt acggttctgg aatacagtac aaaatacttc gatcaatagg caaaaattct
      781 gggtgcatga catggatgga gcgattccaa acggatgacc aaaataaagt ggaagtgatg
      841 cttcagaaaa tgggtgtgca atttcgatgg attcaaggca atgggctaat catagaacaa
      901 ttgcttcctg cctgccggaa tcatccgatc agcggaaaac tagtctggtt taatcaaagt
      961 aaccatgcta atcattatta taatggaacc agtgattaca tcaaaagtaa gattaataac
     1021 actttttctc gttttatctt gttacataaa tattttcatc cttatattgc cttttatggc
     1081 gacggtgaac ctttatcgaa gcaagaagca gattgcataa actctgcaat acaaaaaaat
     1141 acggtatcca cagcctggca accaggggat gttatgatag ttgataattt ttcctgcttg
     1201 catggaaaaa caccccatac aggaaaccga ttaatcctgg ttggattgac aaaatatttt
     1261 gattaaaggg acccatacca atgcctgatg atatttcacg aaaacaattt agtattggag
     1321 atttgtattt tactaatgaa ttggaagtca gtggtttgat ttatgagatt ttctatcaga
     1381 aaagctattt gtcggatttt cttactttaa gtccaggggc tgttgttttt gatgtcgggg
     1441 cgaatattgg tgttttttcc ctatttgttt taaagcaatg tcattatgat actgaaattt
     1501 atagttttga gcctgtccct gctactttta agtgtttaaa aaagaatttg gcgcggttta
     1561 agcataacgt gcatgtttac aatgccggga taggaaatgt tccaaaagat tgtagtatcg
     1621 attttaccct ctttggggaa agctccgtta ccgccactta caaaccaact gataaaatca
     1681 tttccaatta tcaaccattg ttaaattatg aaacattgtt aaagctctct tccttccaaa
     1741 ataagccgct ttattatcaa ttaaaatatt tgcctttttt gcgtaattat ctcattaaaa
     1801 aaaactataa acatcagact ttagaaacca aagtgagatg ccacctgact gcattagggc
     1861 gttttattga aaataatcag atcactcgta tcgatttact aaaaattgat gtggaaggtg
     1921 ccgagcttga tgtaataaac agcataaaac cagagcaatt ttcgcttatt aaacaactaa
     1981 gcattgaagt tcatgatatt gataatcggg ttgaaaagct cgtctcctat ctacaaaagc
     2041 aaggttatat tacttatgtt gatagaaacc caatttttgc agagctgggt tttaatcatc
     2101 atatgatcta tgccaaattg cctgaaccgg caattatcac actaagtgaa gaaccaaata
     2161 atcctgagaa ttatattgaa gcacgccagt atttttatgg actccttgct ggtggggtga
     2221 ggattaaact gctggaatct atgtttgatc ttgatttgtt tcgtttgttc accggaaaac
     2281 cttatttatt ggaaaatgac attatcaaga gacttgaact gaaacctgtt cgagcaaaga
     2341 aatggctaca tttgctttgt tgtgaagatt ttttaaaaaa aatcatggtt ggttcgcaag
     2401 tcgggtatca attggcaaaa agtcaattga tgttaggaga ggggtattgg ggatttaaac
     2461 aatattatga tttttattgg cagagaatgg ctaacgaaaa attaagtaat atattgcgtt
     2521 ttaccgatcc aaaattcaat gtttcctggc caccaaaaac tgccgaggaa gctaattttc
     2581 ttgaaacatg gatgacaaaa acagcaacac cacttataca aactttattg gcttgtcttg
     2641 atttaaatca atatcgatcc atattggatg ttgggggcgg ggatggcact attgcctgtg
     2701 cactggctca ggcctaccct catttaaaaa ttaccgttta caatttacct gaatcagcaa
     2761 aaatagctca aaaaaatata gatgcaatgg gattacagaa gcgaattagt gtattttcgg
     2821 gtgattttat taacgatgaa caatttcctg caggatttga tttgatttta ttcgtgcgtg
     2881 ttttgtggga ttgggataat tccagaaaaa ggaaattatt aaatatggcg tatcatgctt
     2941 taaaaaggaa aggccatgtt gctatctgtg aaggctttaa agaactttgc tatgatcttt
     3001 gtctgacatg ggaatacagc tatatttttg cagatggatt tgatgcggaa gtttttaaaa
     3061 cgtcagatga gtacaaaatc atgctgcagc aaataggttt tactcctatt ccaatcaaat
     3121 ccatttctcc ttcggagcca gtgcctggga cggttgtatt ggcagaaaaa tagttttcat
     3181 cagccatact gtttggcata gacccattta acacaactaa gagctcaagg ttttcgtaat
     3241 tttgaaaatt ataaaacgcg gttaaagtgt tgtttactct attatattaa aatcctacct
     3301 caataggatt ttaataattt actattgtat atttgaaatt aatcctacta caataggatt
     3361 aattcgcaaa ttaaatcgaa atcttagatt aagtctactc tagtaggatt atatggctaa
     3421 gcaaataaaa aaattaaaga ctcctaacct tcaacaacca ttaactgcgg aattgttagg
     3481 ggaagtcatt aaagcacgtc gaacccaaag caatttgcgg ttagaagatg cggctgcttt
     3541 atgtggtgtt gccaaagaaa cctttatgaa aatcgagcat gggcaatcga actgtcaatt
     3601 ggcaagtgta ttacaaattt gttctggttt aggaataagt ctttacataa aaccttggat
     3661 ggataatagt gaggatgaaa atgattggag ataagtcagt cttggatgtt tatctcggtg
     3721 aaagtcaaat agccaatatc acacttatgg atgatcaact gtattggagt tatcatgaaa
     3781 gttggcaaca gacagggtat gctatttccc cacaccttcc tatgcatgaa gatattccac
     3841 cattaaatgt gcaacgattt ctaagaaatt tattacccga agggaatcct ttagaagtac
     3901 tagttaatgt ttttcattta agtaaataca atacatttgg acttattcaa gctttagggt
     3961 tggatacacc tgggtcatta gttatattgc catcaaatca agcaattcct aaacatgcaa
     4021 attttcgact agtaactcat gaagaattag aagagcgttt aaatcatcgt gattcttaca
     4081 atttaatcgt ttgggacgga aaaccacgtc tttcggttcc cggagtacaa gataaaatta
     4141 atgtggtgtt aaataaagaa ggacaacttg gttttggaga aggtagcttg tgctctaccc
     4201 atattcttaa atttgaaaca caaaaactat cttatttggt tttaaatgaa ttcctcagta
     4261 tgtggctcgc taaatgctgt gaattgaatg tagccaatgt tcagatgaag cgttttggtc
     4321 agaaccctgc tttattggta gagcgttttg atagaaaatt gattaccact actaatatag
     4381 taaaaagacg gcacttaata gatggttgtc aggcaatgaa tttaccccct gaatttaaat
     4441 atgagcaaaa ttttggaaat agcagggatg tggctcatat tagagatgga gtcagtttgc
     4501 ccaagctatt tgattttgcc aatcaatgta tcaatcctgc caaaaccaag cagcaaatac
     4561 tcgactgggt tttatttaat gttttgatat ttaattgtga cgctcacggt aaaaatatca
     4621 gtttttttgt tggggctaat ggtatttcac tggcaccatt ttatgattta gttaatataa
     4681 aaatgtatcc tgaatttgag catgctctgg cgatggcatt aggggatgaa tttgatggat
     4741 ataatattaa tgcctatcag ttggctgatt ttgcagatac atgtcggcta ccaaggtcgt
     4801 tagtagctaa acatttaaaa tatttgattg gcaaattaac aactgctttg caagaagaaa
     4861 cccaatttaa tttagttggt aacgaagaag actattttaa gaaatatcag gaaattgtca
     4921 tgaagagatg tgagcacttg ttaaagcaga acgaccaaat tacttcaata aagctctagc
     4981 gcttttcccc actatttctt actttaatac ggagcacata agagatttgt atatttaata
     5041 tacaaacaat ctattttaat tataatcaaa attatcatat tatgaccaat aagaataata
     5101 tttattcatt aatattgagg ttttaattat gatttataag ataacattaa ctgaaaataa
     5161 cttggcgcaa gcttttatgt ctatccctca agatgtaatc gctcttgatt tgagtaataa
     5221 tctcctgggc tcaaaaaatt gtgtagattt attagtaaat atagcggact gtatacctcc
     5281 gggggtgact tcgcttgatt tgagtagcaa tgggctcaga ttaacgcaag tctctttagt
     5341 tacacccaaa cacgtgactt cacttaattt atgttttaat cccttacatg aaatatctat
     5401 tgacggttta gagtggctta aggattcgtt agcacatatt caaattattt atttaagcta
     5461 tgatatggtc aataacatgt caagagagca acgacaggca tttcgagcgg taattcccaa
     5521 catacaaaca attattttgg tcgataaaaa tggcaaagaa attggtcctt cacatacagc
     5581 aaaaatcgct aatcccatta gagagcttgg aggtggaaac ccagaggttc cctctttgtt
     5641 aaaccaatgt accttttttg ttaaaactca aggaataaat atagataagg cctatattcc
     5701 cgaagaatta aaaaaacgta ttcacgattg caatcctctc tgatttattg ttgcgtagta
     5761 gcaagtgtag gtcgggcctt ggcccgacaa cttttgctaa ttatctaaga tattcgagca
     5821 ggaaagggga aaaaatgaaa aaagtcccag gattaaataa agtaaaaact gatcctggat
     5881 gagcatttgg tctttttttc ttggacgaag tatataaaat gcaacaaaaa gacaggcaac
     5941 accaatcaat atgcttaaaa tttgtactaa caaaaatata gacataaatt gtggagaagt
     6001 ggtctgggca atgtgctctt ctaccggggt gactttggca gcatatgaat tcatttttta
     6061 gcttaagtaa agagattgtt ataatcctcg ctccatttaa ccaagatttc aagtgcatgg
     6121 gaacaatctt tctctacgat tagaaaaatg ctggctttta tgatcatcgc tttggcagga
     6181 ctcataacat agggaagggg tagacaaata aacgccaaat taagtggata actgtaaaaa
     6241 ccaaaaagat ccatgagcca gcttcttcaa atactacctt gatttattca cgataaactc
     6301 agcaactttg caataaaagc cgctgagttt aggaaaatta tcggaaaatt tgcctattga
     6361 aaaaaataaa caacagttgg cgcatgaaat tttgctctac accgaggtaa attcattttt
     6421 ttgatagata ggttaataaa atcataattc ttaaatgcta aaatgacata tttttaagca
     6481 attattaaag gatagtatgt ccaggtctaa attttttaaa tcaaatcgaa cgcatgtaat
     6541 tgagctttat tgttatagtg atgaatatgc gcaacaagtt aatcatgaga taacttcagg
     6601 tgcagattct ggcccacttt taacaaaaat atatggccaa gatgttagat ttatctatgc
     6661 tcctgattcg gaaaaattta atctggtttt aaacgaggcc aggaaaagaa attataacca
     6721 accaataatc aatctttatg aaccagataa tattaaatac ttattatctc gattaagtca
     6781 tggcgattcc atacttatta acgggcaggg tgatattcat aagcaactaa ttgcgggacg
     6841 caatgctgag gagttggtag atattcttga aaatgatttg gagttaaaag aaatatcact
     6901 aaaaaattta gatatagatt cttgcaggat gggaagagtt gaaagttatc gtcatgaatt
     6961 aaaacaccat ttaaagaatt ttcaaactgt tacaacatat actgatttat gcacagcatc
     7021 acaaaggggt ggagtgcctt acagaatgtg gatagaagaa agagcggata gagatgtttt
     7081 ttatactgaa agtgatctaa acaaaaaggg gacccgcatt attgaatata cggataccta
     7141 taaaaattca ttaaaagaaa tttggaaaac aaacccgtat aatctggaag aaattgatct
     7201 gtctgatcat attgatattt tagtaattgc atcatgctaa tttcgacaat agcttgaatt
     7261 gggttgggta actcgcaaat ttttcaatct ttaaactgtg tgatgcgtta agtcataaca
     7321 tctcagactg atccttcctc gaaaagccgg acaccaacat taagcaacca taccaaaatt
     7381 attagagatt tgaaatccaa agaagaatgg atacgttgcc aattttaaaa cacatgaaca
     7441 aaccatctcc acataatatt agtttgccta tttatcaggc ataggcttgc tcgctatttt
     7501 agttgttcca aacaagatgg ctatagttcc aaatttaaaa acctcactaa ggcaggagag
     7561 ttattgttta agcaaaaagt ttaatcaagc agtgggtcaa aataaa
//
LOCUS       DATNSN010000153         7548 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.153, whole genome shotgun sequence.
ACCESSION   DATNSN010000153 DATNSN010000000
VERSION     DATNSN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.153"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            12..872
                     /gene="vipF"
                     /locus_tag="VAH76_002584"
     CDS             12..872
                     /gene="vipF"
                     /locus_tag="VAH76_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector N-acetyltransferase VipF"
                     /protein_id="HEO1387180.1"
                     /translation="MITQVNQLDELQLKDLKTLRAECKKNDGSIPNLYIHILKQHRSL
                     PTSFLYYQNGALIGFLSVYFFYDDAVEVAVLVSPQYRRQGVAKQLIKEALPLIKSQNY
                     FNLIFSCPSRLNDNWLTQKGFTYLHSEYFMERDDLNPILDYIRPLSFRMATLEDIPIL
                     CGLDEVCFPDKNQDSVNRFQQILNEREYEIVIAMLNNHPIGKAHIRWQTKRATLSDIA
                     ILPKEQGKGFGSALIAHCINMILSEGKSRVDLDVETHNKKALNLYIQLGFHIQNACDY
                     WSINVNQLAK"
     gene            997..2226
                     /locus_tag="VAH76_002585"
     CDS             997..2226
                     /locus_tag="VAH76_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO1387181.1"
                     /translation="MKKRVVITGMEITSSIGSGIDKFWLAASQGQCGIKRIQSYDPSP
                     YSTQIAGEITDLSLDHLPEFNKSKRYPRAAQYALFCAHHAIEKAGLEQAELNAAGTFI
                     GTSIGGQPELEAGYKLFFTDSWKKIPPLSVIRGMPNSIANHIAIAFGIGGPNSTISNA
                     CVSSGEAIGMAYQQIAHGNLTTALCGGTESLVWESVMAAWCKLRVMSTNNENPSQASR
                     PFDLNRDGMVMADGAGILILEELQQAKARGAQILGEIIGYGASCDAFHVTAPNSQGQA
                     RAIVAALNDAKLSINDVHYINAHGTGTQLNDSTETETIKMVFGKRAYDIPITAQKAMT
                     GHAIGAAGAMEIIATTLSLQKNTLLPTINLHTPDPACDLDYVPNAAREKTIDIALSNH
                     FAFGGANAALVLRKYIS"
     gene            complement(2223..3005)
                     /locus_tag="VAH76_002586"
     CDS             complement(2223..3005)
                     /locus_tag="VAH76_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1387182.1"
                     /translation="MLKKILLTGLCVLFSMNLSASEPIYDEEIPLLWSSIITVSGGPS
                     WATPGQNQYIYPMSPIYINNFNPNLPPNYYLYFTHDSPTTILANGEIFFGLQRAINPQ
                     MIGQLGLGVAGVSDAKITGDVNLNGLLNVYTYDYKVNHARVELKGKLIGSAFTPVQPY
                     LSGSFGIAFNNAHDYRTLSINQFLFPAPWFASNTTYAFAYSVGAGVQKMLNRNWQVGV
                     GYEFADLGKSYLGGDGVNITKGPRLTHLYNNQLLFSVSYLYS"
     gene            3269..4324
                     /gene="lpxD"
                     /locus_tag="VAH76_002587"
     CDS             3269..4324
                     /gene="lpxD"
                     /locus_tag="VAH76_002587"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEO1387183.1"
                     /translation="MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGE
                     DNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTA
                     VIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
                     NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATL
                     GATVIGEGTKIDNLVQIAHSVKLGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVH
                     IEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTE
                     QVAVINKKLDIKAKEVE"
     gene            4327..7017
                     /gene="polA"
                     /locus_tag="VAH76_002588"
     CDS             4327..7017
                     /gene="polA"
                     /locus_tag="VAH76_002588"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO1387184.1"
                     /translation="MKPPLILIDGSSYFFRAFHALPPLTNSKGQPTGAIYGVANMVKK
                     IIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPL
                     LIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG
                     VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLAHANEIG
                     GKIGEYLRASIPHLPLSKKLVTIKTDLELPLNLNQLMPKPADNEQLIKLTRELEFKSW
                     LKELLQEEENQKSPKEMDASNNKSYEIITTHQQLNHWLNKLEQSQQFCIDTETTNLDV
                     MQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKY
                     DYSVLKNYGITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGA
                     KQLRFDQIPVEKAGIYAAEDADITLRLHHKLYPMLDEPLRNVLHDIEMPLLTVLADME
                     IHGVLIDPVILEKHGSRLKEQMKSLEQEAVQLAGKAFNLNSPKQLQEILFDEQKLPVV
                     AKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHT
                     SYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMA
                     HLSQDDNLLRAFANGWDIHAATASEIFQTNLEAVSKEQRRRAKAVNFGLIYGMSAFGL
                     AKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSR
                     NLMRQKAAERTAINGPMQGTAADIIKKTMLAVSSWEHSQKNPSAKMIMQVHDELVFEV
                     RKEAVEECHAIIHKLMEQTVKLSVPLVVSIGIGSNWDDAH"
     gene            complement(7047..7499)
                     /locus_tag="VAH76_002589"
     CDS             complement(7047..7499)
                     /locus_tag="VAH76_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO1387185.1"
                     /translation="MRVLIVEDSAFNAFCLRRLLESVVASISVTIVNNSQAALSFVNN
                     YCPDIVVVDGDLGASPDEMNCNGPELAGILLQKYPQLPIVAWSDSDAMREDFIKVFKS
                     RGLFLNEYNTWPKVIGLERIHKTWSYYFGEFIGGQNPSFASQTSINYY"
BASE COUNT         2467 a         1561 c         1475 g         2045 t
ORIGIN      
        1 ctaatacaat tatgattact caagttaacc aattagatga acttcaatta aaagacttaa
       61 aaacattaag agcagagtgt aaaaaaaacg atggcagcat ccccaatcta tatatccata
      121 tattgaaaca acaccgctct cttcctacaa gctttcttta ttatcaaaac ggggccttga
      181 ttggattctt gagtgtttat ttcttttatg atgatgctgt tgaagtcgct gttctggtta
      241 gtccccaata tcgcagacaa ggggttgcca aacaattaat aaaggaagca ttacccttaa
      301 ttaagtctca aaactacttt aacttgattt tttcatgccc ctcacgcctc aatgataact
      361 ggttaaccca aaaaggattt acctacctgc atagcgaata tttcatggaa cgtgatgatt
      421 taaatcctat actcgattac atacgtccat tgtcctttcg catggcgact ttagaggata
      481 ttcccatatt atgtggcctc gatgaagtat gttttcctga taaaaaccaa gactcggtta
      541 accgattcca acaaattctt aacgaaagag aatatgaaat tgtaatagcc atgctcaata
      601 atcatcccat aggaaaagcc catattcgct ggcaaaccaa aagggctacc ttgtctgata
      661 ttgccatatt acctaaagaa caaggaaaag gatttggttc tgccttgatt gctcattgca
      721 tcaatatgat tttgagcgaa ggcaaatccc gggtggatct tgacgttgaa acccataata
      781 aaaaagcatt aaatctgtat attcagcttg gatttcatat tcaaaatgct tgtgattact
      841 ggtcaattaa tgtgaatcaa cttgcaaaat aaaactgttt attctttcgt tacaaatcac
      901 atattataaa tatggcttga cataattaaa atctcaccat tagtattgcg aatggcgata
      961 gaggaaggct tgttttatca aaaataagga taattaatga aaaaaagggt tgttattact
     1021 ggtatggaaa taacgtcatc cataggatcg ggaattgata aattttggct tgctgcatct
     1081 caaggtcaat gtggaattaa acgcatccaa tcttatgatc catcccccta ttcaacccaa
     1141 atagcaggtg agattacaga cttatctctt gaccatctcc ccgaattcaa caaaagcaaa
     1201 cgttatcctc gtgccgctca gtacgcatta ttttgcgcac atcatgccat tgagaaagca
     1261 gggcttgagc aagctgagct caatgcagca ggcaccttta taggcacaag cattggcggc
     1321 caacctgaac tggaagcagg ttacaagtta ttctttacag acagctggaa gaaaatcccc
     1381 cctcttagcg tgattagggg aatgcccaat tcaatagcca atcatatcgc aattgctttt
     1441 ggcataggtg gaccaaactc aaccatttcc aatgcctgtg tttcatccgg ggaagcgata
     1501 ggcatggctt accaacaaat tgcccatggc aatttgacca cagctctttg cggaggaaca
     1561 gaatctttgg tctgggaatc ggttatggct gcctggtgta agcttagagt aatgtcaact
     1621 aacaatgaaa atccctcaca agccagcaga ccgtttgatc tcaatcggga tggaatggtt
     1681 atggcggatg gggctgggat attaattctt gaagagctac aacaagcaaa agcaagggga
     1741 gcacaaattc tcggagaaat cattggctat ggcgcaagtt gtgatgcatt ccatgtcaca
     1801 gcaccaaact cacaaggaca agcaagagct attgttgctg ctttaaatga tgccaagtta
     1861 tcaattaacg atgtccatta catcaacgca catgggacag gaacccagct caatgacagc
     1921 acagaaacag aaacgattaa aatggttttc ggtaaaagag cctatgacat cccgattact
     1981 gctcaaaaag ccatgacagg gcatgcgatt ggggcagcag gggcaatgga aatcattgca
     2041 acaacactaa gcttgcaaaa aaatacgctg ttaccaacta ttaacctgca taccccagac
     2101 ccagcctgtg atctggatta tgtacctaat gctgcgcgag aaaaaaccat tgatattgct
     2161 ttatccaacc atttcgcttt cggtggagca aatgccgctt tagttctgcg caaatacatt
     2221 agttaagaat atagatagct tacgctaaac agcaattgat tattatataa atgggtcaat
     2281 cgtggccctt tggtaatatt aacgccatca ccacccaaat aacttttccc taagtctgca
     2341 aactcatacc ctacgccaac ctgccagtta cgatttaaca ttttttggac accggccccc
     2401 acactgtaag caaaagcata agtggtgttt gacgcaaacc atggtgcagg aaacaggaat
     2461 tgattgatag aaagagtcct gtaatcatgg gcattattaa atgctattcc aaaactaccg
     2521 ctcagataag gttgcacggg tgtaaatgca cttcctatca gtttgccttt tagctctact
     2581 cgtgcgtgat tcactttata gtcatacgta tagacattaa ggaggccatt caggttgaca
     2641 tcaccagtaa tcttggcatc gcttactccc gctaccccta aacctaattg accaatcatt
     2701 tggggattga tcgcacgctg cagcccaaag aatatctcac cattggctaa aatagtcgtc
     2761 ggagaatcat gggtaaaata aagataataa tttggcggta aattgggatt gaaattatta
     2821 atatagatcg gagacattgg ataaatatac tgattttgtc caggtgttgc ccacgaaggt
     2881 cctccactca ctgtaataat agaggaccaa agcaagggaa tttcttcgtc ataaatcggt
     2941 tctgaagcgg aaagattcat actaaataaa acacataatc cggtgagcaa tatttttttc
     3001 agcatatccc atccttttga ttacaaatca gccctcttga ttcaggctct ttaatgaact
     3061 gattttaata gaagttattt gattttatac cctaattaat ctcatttttc tagggtgcgt
     3121 tgacaattca tctggatcac cagaatagca ggatcatgat aagaaaaatt attaatctga
     3181 aagccatctg aatcgaggat aattcactcg aaaatggaca ataagaggta tataatcgct
     3241 ctattttgcg gaattgaaag gtaaaccaat gaattcttct cttcgtgata tagcaaacct
     3301 tgttcaaggt atggttattg gcaatgatgc catcactgtt tccacactat cacccataga
     3361 taatatactt cccggatctt tagttttcgc cgaaggtgaa gacaatatca aacttgctga
     3421 aaactctgaa gcagcagcta ttcttattgg tcaaggtact atcgattccc caaagccatt
     3481 aattcaggta aaaaaccctt ttaaagcatt tattgcctta ctcaaccatt tttatccccc
     3541 aaggagaata tctccggggg tacaccccac tgctgtaata ggtgccgaag tacagttagg
     3601 cgatgaagtt tatgttgggc cattcgtagt cattgaatca ggaagtatta taggcaacca
     3661 ctcggtgctc aaaagccata ttcatatcgg ccataatgtg gtcattggag atcatacaac
     3721 cattcacccc caagtcacta tttatgataa ttgccgtatc ggttcgaatg tcactatcca
     3781 tgcctctaca gtaataggtt ctgatggctt tgggtatact tttgttgatg gccaacatct
     3841 caaagtacct cattcgggat atgtggtaat tgaaaataat gtcgaagttg gagccaacac
     3901 agcgatagac aaggccacat taggtgctac agtgataggt gaaggtacaa aaattgataa
     3961 tttggtacaa atcgctcact cagtcaaatt aggcaagcat aatattattt gcgcttttac
     4021 tggcattgcc ggcagtacca ctaccggaaa taatgtcatc tttgccgcca acgtgggagt
     4081 gagtgaccat gttcacatcg aggatgaggt catattaggc gcacgtactg gagttccccc
     4141 acacaaacac ttaaaaaaag gaacagtata tcttggtaat ccggcaaaac ctaaagatgt
     4201 tgcgattaag cacgagctgt cagtgaaccg tatcccatta atacgaaaaa acattaaatc
     4261 acttaccgag caagtggctg taattaacaa aaaactcgat attaaagcaa aagaggtaga
     4321 gtaactatga aaccgcctct gattctaata gacggatctt cctatttctt tagagcgttt
     4381 catgcccttc caccattgac aaactccaaa ggccagccta caggcgcaat ttatggagtt
     4441 gccaatatgg ttaaaaaaat aatcaaagat tatcagccag aagaaatagc cgttgtcttt
     4501 gatgccaaag ggaaaacatt ccgagatgaa tggtatccag aatacaaggc gcacagacca
     4561 ccaatgcctc aagaattgag ctctcagttt caaccgctaa tccagttact gcaagcaatg
     4621 ggattgcctc tgcttatcat cgatggcgtc gaagcagatg atgtgattgg cactttggca
     4681 aaacaagcca cagagcaagg cataccagtt gttatatcaa ccggtgataa agacatggca
     4741 caattggtca atgaacatgt ctccctcatc aataccatga gtaattacag tatggatatt
     4801 gatggtgtca aagcaaaatt tggtgtaaca cctgaacaaa tcattgatta cctcactctc
     4861 attggagaca gtgtagataa tattccagga gtggagaaat gcggccccaa aacagctgta
     4921 aaatggctga cagagtatca aacgcttgat aatttgcttg cacacgccaa cgaaattggc
     4981 ggcaaaattg gtgaatattt gcgtgccagc atccctcatt tgccactctc aaaaaaattg
     5041 gtcactatca aaactgattt ggaattgccc ttaaatttaa accaattgat gccaaaaccg
     5101 gctgataacg agcaattaat taaattaact cgtgaattag aatttaagtc atggcttaaa
     5161 gagttactac aggaagaaga gaatcaaaaa tcacccaagg aaatggatgc cagcaataat
     5221 aaatcctatg aaataataac gactcatcaa cagttaaatc attggcttaa taaactagag
     5281 caaagtcaac aattttgcat cgacacggaa acaaccaacc ttgatgtcat gcaagctgaa
     5341 ttggtaggca tctctttagc agttgaggaa gaaaatgcat cttatattcc ccttgctcat
     5401 actgatggca gtacacagtt agtaaaagaa gaagttttaa ctgctttaaa gcctatttta
     5461 gaaaatccag ctataggaaa aatagggcaa aatattaaat atgattacag tgtccttaaa
     5521 aattatggca ttaccttaaa aggcattagg tacgacacca tgctggaatc ttatgtactc
     5581 aatagtggtg caggcagaca tgatatggat tctttggctt taaaatatct gggttataaa
     5641 acaataagtt atgaggatgt tgcgggaaag ggagccaaac aactccgttt tgaccaaatt
     5701 ccagtagaga aagctggaat ctatgcagcc gaagacgctg acattacttt gcgattacat
     5761 cataaacttt accccatgct ggatgagcca ttgcgtaacg tgttgcatga tatcgaaatg
     5821 cctttactca ctgttctcgc agacatggaa atacacggcg ttctcatcga tccagtcatt
     5881 ctggaaaagc acggaagtcg tttgaaagaa caaatgaaat ctctggaaca ggaagcagtt
     5941 caactcgctg gaaaagcatt caatctcaat tccccaaaac aattacaaga aatactattt
     6001 gatgaacaaa aactgcctgt tgtagcgaaa acccccacgg gacagccttc gactgcagaa
     6061 tctgttttac aggaattggc ttttgattat cgccttccgg ctgtaattct ggaataccga
     6121 agcttatcaa aattggtcag tacctatatt gatgccttac ccaaaagaat caaccccaaa
     6181 acacataggg ttcacacatc ctataatcaa gcggtagcag caacaggccg attatcttcc
     6241 agtgatccta acttacaaaa tatccctata cggagtgaag aaggaaggtt gattcgaact
     6301 gccttcatag caccagaagg aagtcttatc atggcggctg attactcgca aatcgagtta
     6361 aggataatgg cgcatttatc tcaagatgat aatcttttac gagcttttgc taatggttgg
     6421 gacatccatg ccgcaacagc gagtgaaatt tttcaaacga atttggaagc tgtatccaaa
     6481 gaacaacgaa ggcgcgctaa ggcagtgaac tttggcttga tatatggtat gtctgccttt
     6541 ggactggcaa aacaaattgg tgtggagcgt caggatgctc aacattatat tgatacttat
     6601 ttccgccgtt atcctaaggt acttgaatac atggagcgta ctcgcaaaca agcgcatcaa
     6661 cttggttatg ttgaaacctt gtttggccgt cgtctctact tacctgaaat taattcacgc
     6721 aatctaatga gacaaaaagc agctgagcgc actgccatca acggaccaat gcaaggaacc
     6781 gcagctgata ttataaaaaa aaccatgctg gctgtttcca gttgggagca ttcgcaaaag
     6841 aacccctctg caaagatgat catgcaggta catgatgaat tggtttttga agtccgaaag
     6901 gaagcggttg aagaatgtca cgccattatc cataagctca tggaacaaac tgtaaaatta
     6961 tctgtacctc ttgttgtgtc tattggcata ggttctaact gggatgatgc gcattgaagc
     7021 gcataaaaac gtttaattta ttggatttaa taataattaa tagaagtttg actcgcaaag
     7081 gaaggatttt gaccacctat aaactcacca aaataataag accacgtttt atgaatacgt
     7141 tccaaaccaa tgactttggg ccaagtatta tactcattca aaaacaggcc acgactctta
     7201 aacactttaa taaagtcttc tcgcatagca tcggaatcag accaggctac aataggaagt
     7261 tgcgggtatt tttgcaataa aattcctgca agctcgggac cgttgcagtt catttcatca
     7321 gggctggcac ccagatcacc atcaacaacg acgatatcgg ggcaatagtt attgacaaag
     7381 gataaagcgg cttgactatt attgacaata gtcactgaaa ttgatgcaac aaccgactcc
     7441 aacaatcggc gcaaacaaaa ggcattaaaa gcactgtcct caacaattaa tacacgcatc
     7501 actgctccct cttaactacg agagaagtat aatgtgcgaa ccttaaga
//
LOCUS       DATNSN010000154         7369 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.154, whole genome shotgun sequence.
ACCESSION   DATNSN010000154 DATNSN010000000
VERSION     DATNSN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.154"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            129..3359
                     /locus_tag="VAH76_002590"
     CDS             129..3359
                     /locus_tag="VAH76_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387186.1"
                     /translation="MPYTLTLLGTDTQFSPNRLEGAYDKAETLSYVSTLVSNKQPQDR
                     TFPTDEIVKYRTSKIAVVDGPTTLGTEVGDRIARGVEAILEAISRGETDISIIAHSRG
                     AVEAILVAHELERIQSLVEKGNFNRYQLTNSECRYTNRAMNRDANHTKAFDSLDLEKI
                     ANNIGRVKISMFNIDPVPGGNYMGITHASSLAWRDPRFYSIPKIVKEYEQYTYENERT
                     RCFKPIVPKCASTETHFKLHTLPGHHGTGSGNLLDQQRGNIPSDKTTEHVQELVVVKL
                     LDFLTRNNVTIRPKSSEEHDPFANITDQLFNGESIDRGKLKSLFFNLYEEISRNREAY
                     QHFNRTSYAVLGQEQAILRRIWNITDQRIVHYQAHNDTYLDTVVPPVPGGHFLNYEHA
                     RLHLNQELGLEEGRPLSETINNAVDRLISVCRHTHQLKDLRVSGAAIDPTASVLLDKI
                     APTLDTREGFDLFLEGLGMLIDEVRRPYLQGELELINPEERASLYSAIVRAFESFNKY
                     THDNPQNELAKSILSSLNSNLESTLETKRKKLDERYETLSMKLRGKGFLTALQNRIKE
                     IKTNLNEKSTGLDSSEYELDLKLQELLIQTEKLSNSRVEEIKETFEQALQSFREVRFT
                     SELARNTQEWTCLVLDEAIDESLNYSVESLMSEVIKSYNELDNFKKTLPDFKILYDSL
                     SYAEWESNLERKRDHMVHLAARYIAHEGLDLEKDIKPFFPHDSAIYLQIEALAIGLGA
                     RNPHIIRLLDENRLNLEKIDELVLIQDQQSKAIKVLTDNTIQQESLIEQLREREKELY
                     SVNNELRLMSQEKTGESEQLVKKKEQLEMDVRNLKQKTQEHKKVIDELSQQIVALNNQ
                     IVELKLKNEEQTHRISGLEAEKIQEKQRSQTAENNAQAELIQQLLSPKEISCANLIEA
                     QLVPSTNDYLHHLIEQAKKINPLVTDNIYEKLPPFNGSEADKSNYEKIVAKYDITKKM
                     SDILNDKENIPLPSSRIKKFTETLQRNDKTLAEHRDPEWKRYVKNCLIAIGVICTGII
                     PGIVALMAYSTLKGKSSPMFFTNSAGKEYTDKVEKSLTQLPSGPRK"
     gene            complement(3500..3575)
                     /locus_tag="VAH76_002591"
     tRNA            complement(3500..3575)
                     /locus_tag="VAH76_002591"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3539..3541),aa:Glu,seq:ttc)
     gene            complement(3632..3707)
                     /locus_tag="VAH76_002592"
     tRNA            complement(3632..3707)
                     /locus_tag="VAH76_002592"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3671..3673),aa:Glu,seq:ttc)
     gene            complement(3746..3821)
                     /locus_tag="VAH76_002593"
     tRNA            complement(3746..3821)
                     /locus_tag="VAH76_002593"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3786..3788),aa:Ala,seq:ggc)
     gene            4237..5769
                     /locus_tag="VAH76_002594"
     CDS             4237..5769
                     /locus_tag="VAH76_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HEO1387187.1"
                     /translation="MRRVLIYIIFFSYFKLGFTHPQMEFQSNMQEINQYIEKTWSILT
                     RDNANLLADSDEKLPHTQSIIYLPPEENLNKIKNTITQNWPEWKKKKVIFKYLPHDIS
                     TIKQHGLLYLPHPYIVPGGRFNEMYGWDSYFIELGLIEHNRLTMARHMIDNLIYEIDH
                     YGTILNANRTYYLQRSQPPLLTAMILAYYEKTQDKKWLASTLPAIKKLHTYWTTPPHL
                     IPDLGLSRYYAGGQGKPPEESTVYYEKVLNYFKTHDISDYDKSLFYTETSNQLTELFY
                     IADRTVRESGFDITAKFGPFSAGILDYAPVDLNVLIYQMENDLGIIYKILGNKENATI
                     WTQKAEARAALINHYLWDDKAGYYFDYNFKTKQLRPYIYATTFYPLWAGIASKGQAQS
                     LVNNLPVLLTRGGLLTSCYIQGVQWDAPFGWAPLQYFAVLGLNRYGYKELALDIANRF
                     VNTIHIGFKEAHTLFEKYDVQNMSIHTENKIQYSYSTNVVGFGWTNGVYLVFNRLINA
                     NN"
     gene            complement(5790..6854)
                     /locus_tag="VAH76_002595"
     CDS             complement(5790..6854)
                     /locus_tag="VAH76_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387188.1"
                     /translation="MQILKSLSEGMGTAGGVAWTTFGLLFGSFGLTVGNSALTISLGV
                     ISASLFLLVAIPVTYWSYCNSHKENDELRHAIEEQENQIFHYLTEYLLAIRQECLLKN
                     PQKYRNHYDKSVLAEDLENRLLKTRSKRPVSFTTAQFLDCLLDDNHKNSILGMLTDAC
                     TMDKLVLKKKLLAIYRDSVFLQLEELSLFAKLKTGFIAFCAGFGSLAGCSSGSLGLFA
                     ALGIFPGFAAVPLVGLAIMGIACVFGLFLSGMSIDTAIQRSKEKQLFVQSEAIHHDLK
                     NLATLKTASVKAELKAQTKYQIRAKEGLTEKLARPKLLKRSSSFDSYFYRNHPLWSIS
                     HNSPEASKCSFENLGSSFSL"
     gene            complement(6946..7368)
                     /locus_tag="VAH76_002596"
     CDS             complement(6946..7368)
                     /locus_tag="VAH76_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO1387189.1"
                     /translation="MVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADI
                     EQVQTSYGPMLELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVINRPPRD
                     GHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW"
BASE COUNT         2588 a         1395 c         1300 g         2086 t
ORIGIN      
        1 cacaattttt tacaaagtta ttcccttgct taaataataa ataaactcac tccgagccgc
       61 attatttggt gtttttttat tcttattgta tatttattat aacattagtt cttctgttgt
      121 ctgatattat gccatacaca cttactttat taggtacaga cactcaattc tctcctaatc
      181 gcttagaagg tgcatacgat aaagctgaaa ctttaagtta tgtatcaact ttagttagta
      241 acaaacaacc tcaggataga accttcccta cagatgagat agttaaatac agaacttcaa
      301 aaatagcagt tgtagatggt ccgacgactc tgggaacaga agttggagac cgaattgcgc
      361 gaggtgttga agccatacta gaagcaatta gtcgcggtga aacagatatc agcattattg
      421 ctcatagccg aggagctgta gaggcaatat tggtagccca tgaattagag cgcattcaaa
      481 gccttgttga aaaaggcaat tttaatcgtt atcaactgac taatagcgaa tgccgatata
      541 ctaacagagc aatgaataga gatgctaacc ataccaaagc atttgattcc ctggatcttg
      601 aaaaaattgc taataatatt ggtagagtca aaatatcaat gtttaatatt gatccagtac
      661 ctggcggtaa ctacatgggc atcactcacg cttcatcact cgcatggaga gatcctcgtt
      721 tttactcaat accaaaaatt gttaaagaat atgaacagta tacttatgaa aatgagagaa
      781 cgcgttgctt taaaccgata gtgcctaaat gcgcatccac agaaacccat tttaaactcc
      841 atactcttcc tggacaccat ggaacaggct cgggaaacct actggatcaa caaagaggca
      901 acattccctc agataaaacc actgagcacg tacaagaact agtggtagtc aagctcctgg
      961 attttttaac tcgtaataac gtaactatta ggcctaaatc atccgaagaa catgatccat
     1021 ttgcaaatat taccgatcaa ctttttaatg gagaatccat tgatagagga aaattgaaaa
     1081 gtctattttt taatctttat gaagagatta gtagaaaccg agaagcatat caacatttta
     1141 atagaacctc ttatgctgtg cttgggcaag agcaagcaat actaagacga atatggaata
     1201 ttactgatca acgtattgta cattatcaag ctcacaatga tacatatttg gatacagttg
     1261 ttcctcctgt acctggcgga catttcttaa actacgagca tgctcgtctt catttaaatc
     1321 aagaattagg attggaagaa ggaaggccac ttagcgaaac aataaataat gcagtagata
     1381 gattgattag cgtatgcaga catacccatc agttaaaaga tttgagagta agtggagctg
     1441 caattgatcc tacagcttca gttttattag ataaaatagc accaaccctt gataccaggg
     1501 aaggatttga tctttttctg gaaggtttgg ggatgctaat cgatgaagtg agaagacctt
     1561 atttgcaagg tgaattggaa ttaataaatc cggaagaaag ggcatctctg tattctgcta
     1621 tagtcagagc atttgagtca tttaacaaat acacacatga caaccctcaa aatgaactgg
     1681 caaaatccat tctaagctca ttgaactcaa atttggaatc tacactagaa actaaacgca
     1741 aaaaactcga tgagcgatat gagacgcttt caatgaaact aagaggtaag gggtttctta
     1801 ctgctttaca aaatagaatt aaagaaatta aaacgaatct aaatgagaaa tcgactggac
     1861 ttgactcttc tgaatatgaa ttagatctaa aattacaaga actcttaatc cagacagaaa
     1921 aattatctaa ttcaagagta gaagaaatta aagaaacttt tgaacaggca cttcaatcct
     1981 ttcgagaagt acgttttaca agcgaattag caagaaacac tcaggaatgg acctgtttag
     2041 ttctagatga agccatagac gaaagcctaa attatagtgt agaaagtcta atgtcagaag
     2101 tcattaaatc atataatgaa ctagataact ttaagaagac acttcccgat tttaaaatac
     2161 tttatgattc attgagctat gctgaatggg aatccaatct agaaagaaaa cgagaccaca
     2221 tggttcattt agctgctcga tatatagcac atgaaggctt ggatctcgaa aaagacatta
     2281 aaccgttttt tcctcatgat tctgcaatct atctgcaaat tgaagcactt gcgatcgggt
     2341 taggggcaag gaaccctcat atcatacgat tattagatga aaatagatta aacctggaaa
     2401 aaattgatga attggttttg attcaggatc aacaatcaaa agcgatcaaa gtattaacag
     2461 ataatacaat acagcaagaa tcccttattg agcaattaag agaaagggaa aaggagttat
     2521 attcagtaaa taatgaatta cgccttatga gtcaggagaa aactggggaa tcagaacaat
     2581 tagtcaagaa aaaagaacaa ttagaaatgg atgttcgaaa tttaaaacag aaaacccaag
     2641 aacacaaaaa agtgattgat gaattatctc aacaaatagt agctctaaat aatcaaatcg
     2701 tagagctaaa acttaaaaat gaagaacaga ctcatagaat tagcggattg gaagctgaaa
     2761 aaatacaaga aaaacaaaga tcacaaactg ctgaaaataa tgcgcaagct gagttaattc
     2821 aacaactgct ttcacctaaa gaaattagtt gtgctaatct tattgaagcc caattagtac
     2881 catcaaccaa tgattatctt caccatttaa tcgaacaagc aaaaaaaatt aatcctttgg
     2941 tcactgataa tatctatgaa aaactgcctc cattcaatgg ctctgaagct gataagtcga
     3001 actatgaaaa aatagttgct aaatatgaca ttacaaaaaa aatgagtgac attttaaatg
     3061 acaaagaaaa cataccatta cctagttcac gaataaagaa atttactgaa accctccaaa
     3121 gaaacgataa aaccttagct gagcatcgtg atccggagtg gaagcgttat gtaaaaaatt
     3181 gtctcatagc tattggggtt atttgcactg gaatcatacc tggaatcgtt gcgttgatgg
     3241 cttatagcac gcttaaagga aaatcgtctc caatgttctt tactaatagt gctggcaagg
     3301 aatacacgga caaagtagag aaatcgctta cacaattacc gtctggccct cggaagtaac
     3361 agttaagaag aatttgggca acttacggtt atattatatg tggctgacta aagtactcct
     3421 aaaattgaga tgagccagta taatttatcc aaggtaagga aagacttgaa gcatataatt
     3481 ttttaggtaa acaattatat ggcgtcccca gggggattcg aacccccgtt accgccgtga
     3541 aagggcgatg tcctaggcct ctagacgatg gggacctgga tcttattaag attacatcaa
     3601 aacactacta cactatttaa atgccaaata atggcgtccc cagggggatt cgaacccccg
     3661 ttaccgccgt gaaagggcga tgtcctaggc ctctagacga tggggaccta gtactttttt
     3721 taccatcaac atacaacgat gttgatggtg gagctaggcg ggatcgaacc gccgacctct
     3781 tgcatgccat gcaagcgctc tcccagctga gctataaccc caaaataagg tgtgcagttt
     3841 aattagacgc attaatactg tcaagtggtt tttctatttt aatgcaagtc ttttataaga
     3901 caaccttttt ttacatcaaa atcataaata aatccaaatc acagcgctaa ttttatcaat
     3961 agaaatgaac atgagtaaaa gcatcattgc agcaaaaatt tatgactgta agagtctaat
     4021 ccaaaaaata tttgaccagc acgaaaacaa aggttgtaat agatctttac ataacctgat
     4081 tattttgttt taaggctgag caccagaaaa gtttgtaact cagcaataaa atgaaagaga
     4141 caggttaagc aaaaagtcta ataaacatca gactttaaat tctgtttaaa ccaggaaagt
     4201 taaacatttt ttaatcacaa aattacaggg aaacggatgc ggcgtgtttt aatctatatt
     4261 attttcttct cttactttaa gcttggcttt acccatcccc agatggagtt tcaatcgaac
     4321 atgcaggaaa ttaatcaata catagaaaag acctggagta tactaaccag agataatgcc
     4381 aatctcttgg ctgactcaga tgaaaaacta cctcacactc aatcaatcat ttacttaccg
     4441 ccagaagaaa atctcaataa aataaaaaac acaatcaccc aaaattggcc tgagtggaaa
     4501 aaaaagaaag tcatttttaa ataccttccc cacgacatct caacaataaa acagcatggt
     4561 ttgctgtatt tacctcatcc ctatattgta cccggaggaa gatttaatga aatgtatggc
     4621 tgggatagtt attttataga gttgggtcta atagagcata atcgtctgac aatggctcga
     4681 cacatgattg ataatctcat ctatgaaatt gaccattatg gaactattct aaacgctaac
     4741 agaacctact acctccaacg ttcacaaccc cctttactca cagcaatgat tttagcttat
     4801 tatgaaaaaa cccaagataa aaaatggctt gcatcgactt taccagccat caaaaaactt
     4861 catacttact ggacaactcc cccccaccta atccctgatc ttggtttatc tcgttattat
     4921 gctggtggcc aaggaaagcc cccagaggaa tcaacagtat attatgaaaa agttttaaat
     4981 tattttaaaa cccacgacat tagtgattat gataagtccc ttttttacac tgaaacatca
     5041 aaccagttaa cggagctgtt ttatattgct gaccgaacag tcagagaatc cggtttcgac
     5101 attactgcca aatttggtcc tttcagcgca ggcattctgg actatgctcc agtagatctg
     5161 aatgtgttaa tttatcaaat ggaaaatgat ctgggtataa tttataaaat ccttggcaat
     5221 aaagaaaatg ccactatttg gactcaaaaa gccgaggcta gggctgcact aataaaccat
     5281 tatctttggg atgataaggc tggatattat tttgattata attttaaaac aaaacaacta
     5341 agaccctata tttacgccac tactttctat cccctgtggg caggtattgc atccaaaggc
     5401 caagctcaat cgcttgtgaa taacctgccc gttctattaa ccagaggagg gttattaacc
     5461 agttgttata ttcagggagt gcagtgggac gcgcctttcg gatgggctcc attgcaatat
     5521 tttgccgtac tcggtttgaa tagatatggg tataaagagt tagcattgga tattgctaac
     5581 cgctttgtca acacaattca tataggattc aaggaagccc atactctttt tgaaaaatac
     5641 gatgttcaaa acatgagtat tcataccgaa aacaaaattc aatacagcta tagtacaaat
     5701 gtcgtaggtt ttggatggac taatggcgtg tatctcgttt tcaataggtt aatcaatgcg
     5761 aataattaac ttataatcaa ttgtttttac taaagtgaaa aagaagaacc aaggttttca
     5821 aagctgcatt tcgaagcctc tggagagtta tgagaaatgc tccataaggg atggtttcta
     5881 taaaaataac tatcaaatga agaagatcgc ttcagcaact tcggccttgc taatttctct
     5941 gtcaatccct ctttagctct aatttgatat ttggtttgtg cttttaactc ggccttaaca
     6001 gatgcagttt tcaacgtggc caaatttttt aaatcatgat ggattgcttc actttggaca
     6061 aataactgtt tttctttact cctttggatt gcggtatcaa tactcattcc agacaaaaac
     6121 aaaccaaaaa cacaagcgat acccataatt gccaacccaa ccaaaggaac tgcagcaaaa
     6181 ccggggaaaa tacctagggc tgcaaacaaa cccaatgaac cactggaaca accagctagc
     6241 gacccaaatc cggcacaaaa cgcaataaat ccagttttta atttggcaaa tagggaaagt
     6301 tcttcaagct gaagaaaaac actatcacga taaatagcca atagtttctt tttcaatacc
     6361 aacttatcca tcgtacaggc atctgttaac atacccaaaa tagaattttt atgattgtca
     6421 tctaatagac agtctagaaa ttgtgctgtc gtaaacgaaa caggccgttt tgatctggtt
     6481 tttaataatc gattttcaag atcttcggcc agcacagact tatcataatg attacgatat
     6541 ttttgaggat ttttaagcaa acattcttgc ctgattgcca ataaatattc cgtcaaataa
     6601 tgaaagattt gattttcctg ttcttcaata gcatgtcgta attcatcatt ttctttatgc
     6661 gaattacaat aagaccaata agtaacaggt attgcgacta ataggaataa actggcagaa
     6721 atgacgccca atgaaatagt cagtgccgaa ttgccaacag ttagtccaaa agaaccaaat
     6781 agcagtccaa aggtagtcca tgccaccccc cctgctgttc ccataccctc tgatagtgat
     6841 tttaaaattt gcatcgattg attcaacatt gccatttggc ccgaaatact attttagtat
     6901 gattaaatta tttccactat tcactaccag aatgaaattg tccggttacc aatgtccaat
     6961 attgggtgca gataaccaag gttcctgctt tggcaaaggc tcaccctttt gcaataattc
     7021 gatagaaata ttatcaggag aacgaataaa agccatgtgg ccatctctgg gtggcctatt
     7081 aattacgaca ccacattcct gtaaatgttg gcagatttta tagatatcat ctacatgata
     7141 tgccaaatgg ccaaaatttc ttccctcagt gtaatcctcc ggatcccagt tataagtcaa
     7201 ctctagcatt ggtccataag aagtttgaac ttgctctata tcagcaggag cagctaaaaa
     7261 taccaaggta aaacggcctt tggcattttc agttcgtttt acttctatca aacccaattt
     7321 attacaataa aaatcgagtg attgttctaa attcgaaact ctaaccatg
//
LOCUS       DATNSN010000155         7313 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.155, whole genome shotgun sequence.
ACCESSION   DATNSN010000155 DATNSN010000000
VERSION     DATNSN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.155"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(241..1590)
                     /gene="dsbD"
                     /locus_tag="VAH76_002597"
     CDS             complement(241..1590)
                     /gene="dsbD"
                     /locus_tag="VAH76_002597"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEO1387190.1"
                     /translation="MRKLIDSFMILLLIFFTQAAWSYTPGFESSNPDTVMHFITGNSA
                     FVYLSAFFGLGILLAFTPCVLPMVPILSGIIVGQNSISTGKAFKLSLSYVTGMAITYA
                     GAGMLAGYMGSTIQTVMQRPVVITLFSVVFVAMALSMFGFFELKFPTSLGNRLNKNNQ
                     LGKQRSYLSVGIMGVLSTLIVSPCVTAPLIGVLSYIGQNGQVIMGGLILFVMALGMGV
                     PLLIVGAGYGTLLPKAGSWMLKIKQLFGFIMLGIAIWMLSRILNQTITHILWAGLLMV
                     ASISLGALKSQNNWRGYLAQGLGILALISGGIIFYNTAIPSVKIKQASVENSPFIKIN
                     TLNALQLQLEQAKKVRKAVFLDFSADWCGDCQEMDTKVFNQPEIQKAMSDWVSIKVDI
                     SDKNAEVDSIKKAFGIYGTPTMIFFNTSGEPITQMTSVGLISLSKMLQLLEQARHLS"
     gene            complement(2132..2983)
                     /locus_tag="VAH76_002598"
     CDS             complement(2132..2983)
                     /locus_tag="VAH76_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1387191.1"
                     /translation="MAMNTKVILITGCSSGIGFDAVFALKNRGHRVIASCRKTEDVQK
                     LLNKGVEALLLDVSNPISIQSAFAQLLIMTSGHLDVLINNAGYGQVGALEDISRDILR
                     QQFETNVFGLQDLTNLVIPVMREQGQGRIINISSILGVVSMPFRGAYNASKYALEGLS
                     DTLRLELSGSGINVITIEPGPINSRFRDNAVDLSLQQIPMEKSYFKNQYRNMLSNFKQ
                     KKADSFFTRDTDAVIKKLVHAVESRKPKPKYPVTFPAYFLIGLKRFFTTRLLDRFILF
                     LSRKELG"
     gene            3137..4645
                     /gene="ravZ"
                     /locus_tag="VAH76_002599"
     CDS             3137..4645
                     /gene="ravZ"
                     /locus_tag="VAH76_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavZ"
                     /protein_id="HEO1387192.1"
                     /translation="MKGKLTGKDKLIVDEFEELGEQESDIDEFDLLEGDEKLPGDSEL
                     DKTTSIYPPETSWEVNKGMNSSRLHKLYSLFFDKSSAFYLGDDVSVLEDKPLTGAYGF
                     QSKKNDQQIFLFRPDSDYVAGYHVDAKSDAGWVNDKLDRRLSEISEFCSKATQPATFI
                     LPFVEMPTDITKGVQHQVLLTISYDPKSKQLTPTVYDSIGRDTYSESLSSYFKGKYRT
                     TCDEILTQSIEKAIKSTDFTLGKFTRAAYNHQNRLTEGNCGSYTFRTIKEVISSSAQG
                     TEVKIPGSGYITSNSYLTSQHVQDIESCIKYRNLGVVDIESALTEGKTLPVQLSEFIV
                     ALEDYGKLRSQQSEKSMLNFIGYSKTAKLTAVELLIGILNDIKGKNEISESQYDKLVK
                     EVDCLMDSSLGKLVQFHLKNLGAESLQKLVLPCVKFDDTIDDFVTIEKDELFDVPDIT
                     GEELASKKGIEQGALDKEALLKQKQIKTDLLDLREEDKTGLKKPLHGGIKVK"
     gene            4949..6346
                     /locus_tag="VAH76_002600"
     CDS             4949..6346
                     /locus_tag="VAH76_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1684 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387193.1"
                     /translation="MKSVDEIDSLLQSAKTSEKDSSEQIVPPNDEIEDGFYLIDDSNH
                     DEDLDFSDFPWLDPDHPLFFPATQANNSKGSTRWDAVNKFSVENWYPHLRKHTFKSHF
                     ITLNYNDIHYLMGNIPPDYDSSKLESIFNKILSEFNNKEVFMRLSTRSPKDSKFLFEE
                     ASTIMSKDFVYWNENDNKHQQLVSFVASMLKAMKIKSGRKIIETIAQSPRVYNDLLAL
                     VSSSNQSDCTTNVILREWHDIRPDHEFRVFVSRRHRKESIVTAISQYFHFLYFDKSPA
                     DCFNFLDEEDKKAVIKKFENYVLKSVDPDVAKFLNFSSEQDDDESSDCIREYIVDLAL
                     IPVSQYHGEITDENIIEIGANTYVMVVIELNPFAPAATGSGLFNWKNDLMMLWGKASC
                     DYPVFKYRTTPREDLQSVSLLPSNYESVIKSALIKRLETSYSNSASQKERFFSSTKPV
                     TESSGSALLDMVTKP"
     gene            complement(6357..7226)
                     /locus_tag="VAH76_002601"
     CDS             complement(6357..7226)
                     /locus_tag="VAH76_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1685 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387194.1"
                     /translation="MQCAIERKNSLRDLQSQPNLGDWMFISSLGSQDFDERKFFSQIS
                     LQSLSFIIRIAKFSAYFAALCEKSEYDELWKKLYSTFGLVLTRDKPVPKSFFTHDGGE
                     INHFNLLRGAYYFHLSQKALAAKEKSFSDSELYWLNQAMKFGSIHASQRYIQFLYQKL
                     DKVASADEAEQILTEAIKLCKANLNQYGSYAYMMLAEAFFRYAMWAQQSGNINRAKSA
                     ITSSINSCTKANQHLRASASSIHNASLGEGLKASNTLAMESPEEAIAFLNAWAVAHLY
                     KHEIELVSRPGFY"
BASE COUNT         2467 a         1336 c         1283 g         2227 t
ORIGIN      
        1 atcaaaattt ttcttctttt ttcacaaaaa ttaattgatt attttttaat aaaattgatt
       61 ttttatatgc ctataaacac gggtttgata aattcttgct tatacaagca actctcagag
      121 caaagaatca aattcatgct tttcaatatt ttgggtacag tttgaatttg atttatttat
      181 caaatattaa ccatacccaa attattaaga acaaatttcg gtagcaaatt gtctattatt
      241 tcaactcaaa tggcgcgctt gctcgagcaa ttgaagcatt tttgatagcg aaatcaaacc
      301 aaccgaagtc atttgagtaa taggctctcc cgatgtatta aaaaatatca tcgttggcgt
      361 tccataaata ccaaaagctt tttttataga atcaacctct gcattcttat cactgatatc
      421 cactttaatg ctaacccaat cagacatagc tttttgaatt tctggttgat tgaatacttt
      481 ggtatccatt tcctgacagt caccgcacca atccgctgag aaatcaagaa atactgcttt
      541 tctaactttt ttagcctgct ccaattgcag ttgaagagcg tttaaggtat taatcttgat
      601 aaatgggcta ttttctacag acgcttgttt tatttttact gaaggtattg cggtgttgta
      661 aaaaataata cctcctgaaa tcaaagcaag aatacccaat ccctgagcca aataaccacg
      721 ccaattgttt tgtgatttta aagcacctaa actgatgctg gcgaccatga gtaatcctgc
      781 ccacaaaatg tgcgtaatgg tctgattcag aatccgggat aacatccata tggcaattcc
      841 caacatgata aaaccaaaca attgttttat tttaagcatc caggaacctg cttttgggag
      901 taaagtacca taacccgcac caacaatcag cagaggaacc cccatcccca atgccataac
      961 aaataaaatc aatccgccca ttatgacctg gccattttga ccaatataac ttaaaacacc
     1021 aattaaagga gcggtaacac aaggagaaac aattaaagtg gataaaactc ccattattcc
     1081 aacagaaaga taacttctct gcttccctaa ttgattgttt ttatttaacc gattcccaag
     1141 gcttgtagga aacttcaatt caaaaaagcc aaacattgaa agagccatgg ctacaaacac
     1201 aacactgaat aaagtgatta ctactggacg ttgcattaca gtctggattg tacttcccat
     1261 atacccggca agcatccctg ctcctgcata ggtgatagcc attccagtca cataactcag
     1321 tgataattta aaggcttttc ctgtagaaat agaattttgt cctacaataa ttcctgataa
     1381 aataggaacc atcggtagaa cacagggcgt aaaagccaac aaaataccta atccgaaaaa
     1441 agcactcaaa taaacaaaag cactatttcc tgtaataaaa tgcatcacag tatccgggtt
     1501 agaactttca aagccaggag tatatgacca cgccgcctga gtaaaaaata tcagtaataa
     1561 aatcatgaac gaatcaatta attttctcat tttaatgtcc caataagtaa taaataacaa
     1621 cgatagatac ttgtatcaga gtacaagtaa cctacactgg acctcttgca tagccagggc
     1681 tggcaagaag cctactgaga aagttgacaa atttactcta gtgggcaatc ggtggtttaa
     1741 aaattgggga tacaggcgat ttgtattttg attgaataac taaaaaataa aggataaatg
     1801 tcaacaagga taagacatta ccgagagata actcctcttc agaaataata taataggcac
     1861 tggacgagca ataattcatc aatgccgtcc tctgagggca ttccgttata gaatgtatat
     1921 attgggtatt actctgagtc tgctgattat tgttatgaaa cactgctggt tttatcatta
     1981 acaaaaccac gctgcatagt atcaggtaat gaattactct gtatttaagt ttcataataa
     2041 ctatagtatc tttttaagag aggtctgtac agtagttgct aattatcaac atcaacttat
     2101 gactatttta taatattacc aataagttgc tttaacccag ctccttcctg gataaaaata
     2161 aaataaacct atctaataaa cgtgtagtaa agaatcgttt taaaccaatt aaaaaataag
     2221 ctggaaaagt tacagggtac ttgggttttg gtttccttga ctccacggca tgaaccagtt
     2281 tttttatcac tgcatcagta tcccgagtga aaaacgagtc tgccttcttc tgtttaaaat
     2341 tgctcagcat atttctgtat tgattcttga aataactttt ttccattggt atttgttgta
     2401 aagataagtc caccgcatta tcacgaaatc ggctatttat agggccaggc tctattgtta
     2461 tgacattaat acctgaacca gacaattcca gtcgaagtgt gtcacttaag ccctccaatg
     2521 catatttgga ggcattataa gcaccgcgaa atggcatgct aactacgcca agtatagagc
     2581 tgatattgat tatgcgtcct tgtccttgtt ccctcattac aggaataacc aaattggtca
     2641 aatcttgcaa accaaatacg ttcgtttcaa attgttggcg aagaatatcc cgagaaatat
     2701 cctcaagagc acctacctga ccgtatcctg cattattaat caatacatcc aggtgacccg
     2761 acgtcataat taacaactgc gcaaatgcac tttggattga aatagggtta cttacatcca
     2821 acaagagagc ctcaacccct ttatttagta atttttggac atcctctgtt tttctgcagg
     2881 atgcaatcac tctatgtcct ctatttttca aagcaaaaac agcatcaaaa cctatcccac
     2941 tggagcatcc tgtgatcaaa ataacttttg tattcatcgc catgacctat gcctaatttt
     3001 taactatcgt actaagctat ctatcgaatt accactatta tgataattat ttgttcaatt
     3061 tttgacttat atttcacttt ttcctaaaaa tcatttgcta aacttttaat atggaaatga
     3121 cagtttagag gtcaaaatga aaggcaagtt aacaggtaaa gacaaattaa tagtggatga
     3181 gtttgaagaa ctgggagaac aggaatccga tatcgatgaa tttgatcttc ttgaaggtga
     3241 tgagaaattg cctggcgact cagagcttga caaaactaca tctatttatc ctcccgaaac
     3301 cagctgggaa gtgaataaag ggatgaacag ttctcgtttg cataaactat acagtttatt
     3361 ctttgacaaa agttccgcct tttatcttgg cgatgatgtt agtgtattgg aagacaagcc
     3421 tttaacaggt gcctatggtt ttcagagtaa aaaaaatgat caacaaattt ttttattcag
     3481 accggattct gattatgttg caggatatca cgttgatgca aaatcagacg ctggatgggt
     3541 aaatgacaaa ctcgatcgtc gtttatctga gattagtgaa ttttgtagta aggccactca
     3601 acctgcaacg tttatacttc cttttgttga aatgcccact gacattacca aaggggttca
     3661 acaccaggta ttgttaacta ttagctacga tccaaaaagt aaacaactca ctcccactgt
     3721 ctatgactcc attggacgag atacctactc tgaatcgtta tcatcctatt ttaaaggcaa
     3781 atatagaaca acttgcgatg aaattttgac ccaaagtatt gaaaaagcta ttaagagcac
     3841 tgattttact cttggtaaat ttacacgagc tgcttataac catcaaaacc gcctcaccga
     3901 aggcaattgc gggtcttata ccttcaggac gattaaagag gttatttcat cctcagcaca
     3961 aggtactgaa gtaaaaattc ctggctcagg atatattact tctaacagtt atttaacaag
     4021 tcagcacgta caagacattg aaagttgcat taaatataga aatttggggg ttgttgatat
     4081 cgaaagtgct ttgactgaag gtaaaacatt accggtacag ctttctgaat ttattgttgc
     4141 cttggaagat tatgggaaat tacgaagtca acaatctgaa aaaagcatgt tgaattttat
     4201 tggctacagc aagacagcaa aattgaccgc tgttgagctg ctaataggca ttttaaatga
     4261 tatcaaaggc aaaaatgaaa tcagtgaatc tcaatatgat aaattagtaa aggaagtcga
     4321 ttgtttaatg gattcatctt taggaaagtt ggtacaattt caccttaaaa atttaggcgc
     4381 tgaatcacta caaaaactgg tacttccgtg tgtcaagttt gatgatacga tagatgattt
     4441 tgtgactatc gaaaaagacg aactattcga tgtacccgat attacagggg aagagcttgc
     4501 ctctaaaaaa gggattgagc aaggtgctct ggataaagag gctttactaa aacaaaaaca
     4561 gattaaaacg gatttgttag accttcgcga agaagataaa accggtctga aaaaaccatt
     4621 gcatggtggc attaaggtaa aatagtgtga attgaattca actatgtgat taataggcag
     4681 aaggtttttt aatgcgctga taatttctta aactttaaaa aataagcgag ccatgctgtc
     4741 atcaattatt gaatttaagt tgaataatta atcatttcat tattaagttc ttacataatc
     4801 attaaattta attcatggtg gctcggtatt tggcaattac aacggaaagt gttagaatta
     4861 attggccata ttgtatccta ataagtcatg ttattggatt atggtgtgaa tattaaattt
     4921 acaaagcgtt gggtggagtg cggtaaacat gaaatctgtt gatgaaatag actctctttt
     4981 gcagtcagcc aaaacaagtg aaaaagacag ttcagagcaa attgttccac caaatgatga
     5041 gattgaagat ggattttatc tcatagatga tagtaaccat gatgaagacc ttgactttag
     5101 tgattttcca tggctggatc ctgaccatcc attatttttt ccggcaacac aggcaaataa
     5161 ttcgaaagga tcgacacgat gggatgcggt aaataaattt tctgtagaaa attggtatcc
     5221 tcatttaaga aaacatacct tcaagtcaca ctttattaca ctaaactata atgatattca
     5281 ctatttaatg ggaaacattc ctccagacta cgatagcagt aaactggaaa gtattttcaa
     5341 taaaatcctt tcagaattta acaataaaga agtcttcatg agattgtcca ccaggagccc
     5401 caaggattcc aaatttctct ttgaagaagc ttctaccata atgagtaagg attttgtgta
     5461 ttggaatgaa aatgacaata aacatcagca attggtgtca tttgttgctt caatgctcaa
     5521 ggcaatgaaa attaaaagtg gtagaaaaat aattgaaaca atagcacaaa gccctagagt
     5581 ttataatgat ttgcttgcgc tcgttagttc aagcaatcaa tcagattgta cgacaaacgt
     5641 tattttgcgc gaatggcatg acattcgtcc agaccatgag tttagagttt ttgtatccag
     5701 acgccatcgt aaagaaagta ttgttacagc gatatcacaa tattttcatt ttttatattt
     5761 tgataaaagc cctgcagatt gtttcaattt tctagatgaa gaggataaga aagctgttat
     5821 taaaaaattt gaaaattatg ttctcaaatc tgtagatcca gatgttgcaa aatttttaaa
     5881 tttttcaagc gaacaagatg atgacgaatc atcagattgc attcgtgaat acattgttga
     5941 tttagccttg attccagtca gtcaatatca cggcgaaata acagatgaga acataattga
     6001 aataggggca aatacttatg taatggtagt catagagctg aatccatttg caccagcagc
     6061 aacaggaagt ggtttattca attggaaaaa tgacttaatg atgctttggg ggaaagcatc
     6121 ctgtgactat cccgtcttta agtatcgtac aacaccaaga gaagatttac agtcagtatc
     6181 attgctgccg tcaaattatg agtctgttat caaaagtgcc ttgatcaaac gattggaaac
     6241 ctcctactca aactcagcaa gtcaaaaaga aagatttttt tcatccacta aacctgttac
     6301 tgaatcctct ggatcagccc tgcttgatat ggttacaaaa ccgtgatagg caagatttag
     6361 taaaaacctg ggcgggagac caattctatt tcatgtttgt aaaggtgcgc gactgcccaa
     6421 gcatttaaaa aagcgatggc ttcctctggg ctttccatag caagtgtatt gctggctttc
     6481 aacccctccc ccaagcttgc attatgaatg gaggaggcac tggcacgcag gtgttgatta
     6541 gcttttgtgc aggaattaat tgacgaggta attgcagatt tagcacgatt tatattaccg
     6601 gattgttgtg cccacatcgc ataacgaaaa aaagcttcag ctagcatcat ataagcataa
     6661 ctgccatatt gatttaagtt tgccttacaa agcttaattg cctccgttaa aatttgttct
     6721 gcttcatctg cagaagcaac cttatctaat ttttgatata aaaattggat ataacgctgg
     6781 cttgcatgga tcgacccaaa tttcattgcc tgatttaacc agtagagctc tgagtcagaa
     6841 aaggactttt cttttgctgc taatgctttt tgggaaagat gaaaataata agcgccacgt
     6901 agtagattaa aatgattgat ttctccaccg tcatgagtaa aaaaagattt aggtactggt
     6961 ttatctctgg tgagaactaa tccaaaagtc gagtagagct ttttccaaag ctcatcatat
     7021 tcacttttct cacaaagcgc agcaaaataa gcagaaaatt tagctattct gataataaaa
     7081 gacagtgatt gcaatgaaat ttgagagaaa aactttctct catcaaaatc ttggctccct
     7141 aaagaggaga taaacatcca atcaccaaga tttggctgtg attgcaaatc acgtaaacta
     7201 ttttttctct cgatagcaca ttgcaatttt ttaagccctc aacatctttg ccaaataaca
     7261 agcatggcaa tatattaaca tattgtacat aatattgcac acatgactta ata
//
LOCUS       DATNSN010000156         7199 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.156, whole genome shotgun sequence.
ACCESSION   DATNSN010000156 DATNSN010000000
VERSION     DATNSN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.156"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(134..1102)
                     /gene="lspK"
                     /locus_tag="VAH76_002602"
                     /gene_synonym="gspK"
     CDS             complement(134..1102)
                     /gene="lspK"
                     /locus_tag="VAH76_002602"
                     /gene_synonym="gspK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspK family T2SS minor pseudopilin variant LspK"
                     /protein_id="HEO1387195.1"
                     /translation="MFMSSPPPKSIASSKMQGSALLTALFIMTLVAIVATAMSTRLQQ
                     DIYRTRLVIAQDKLYLASQAVTFWALNELLDKNNSFTKTNQLGMVAQYPKNMESIYNQ
                     VQLSGGIFDLQARFNLNNLIEKKSIPTLMHLISHAYSKATGQERANIALGVKHWLLAY
                     DLGRGEDIYTSYYLSQKPPYYPSHQLIKSKSEFRLIKDVSAPAYIALEPFITALPEST
                     SININTAPKQILMSLGDGLSDAQANELIIARGENGITDLKEINELLKKFNIPSDQITI
                     ESQYYLSVAYAKNDEFSLVVYSLLKRSRDRKGKLLTSVIRESINNF"
     gene            complement(1089..1706)
                     /gene="lspJ"
                     /locus_tag="VAH76_002603"
                     /gene_synonym="gspJ"
     CDS             complement(1089..1706)
                     /gene="lspJ"
                     /locus_tag="VAH76_002603"
                     /gene_synonym="gspJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspJ family T2SS minor pseudopilin variant LspJ"
                     /protein_id="HEO1387196.1"
                     /translation="MIKNKGFTLIEILIALTVFAILATITSSTLYYAFNTRTRVNAQA
                     ERLNALQLAISIIQQDTSQTVERAIRGNEMRLFPIFVGQPQYLELSRDGVVNPNSVEK
                     RSTLERVALVCQDSKLLRRTWNTLDPIDRNIYKDKVLLDNLSECHFNYLNQNLQLLSE
                     WREQAVNQNQQREPLPKAIQMNLTLKDWGDMNMLFIIPGALYVYE"
     gene            complement(1703..2080)
                     /gene="lspI"
                     /locus_tag="VAH76_002604"
                     /gene_synonym="gspI"
     CDS             complement(1703..2080)
                     /gene="lspI"
                     /locus_tag="VAH76_002604"
                     /gene_synonym="gspI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspI family T2SS minor pseudopilin variant LspI"
                     /protein_id="HEO1387197.1"
                     /translation="MMPKTKLVSGFTLIEVLLALTVIAIALTALLKATAQNTDNTHRI
                     KEKTISHWIAMQGVAMIQLNLLRTSQSQESTQATTMLGQKWYWRAKISPTPIKRMQQI
                     TISVSSKQAGPFREELVAFRYLP"
     gene            complement(2077..2562)
                     /gene="lspH"
                     /locus_tag="VAH76_002605"
                     /gene_synonym="gspH"
     CDS             complement(2077..2562)
                     /gene="lspH"
                     /locus_tag="VAH76_002605"
                     /gene_synonym="gspH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH family T2SS minor pseudopilin variant LspH"
                     /protein_id="HEO1387198.1"
                     /translation="MTTHKGFTLIEILIVIVIIGITLGFALIAFGDFGESRRIQFSAE
                     QLANTLSLAQQQAILESSTLGLKIDNTSYQILKFQDQAGWNPISNKGIFKLYYFPKNV
                     VISLKTNQKHTRGNPAIIIAPSGGMTPFTIEFGTDKEKWIAKLTGSHNGDLDFTMAKS
                     Q"
     gene            complement(2549..2971)
                     /gene="lspG"
                     /locus_tag="VAH76_002606"
                     /gene_synonym="gspG"
     CDS             complement(2549..2971)
                     /gene="lspG"
                     /locus_tag="VAH76_002606"
                     /gene_synonym="gspG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspG family T2SS major pseudopilin variant LspG"
                     /protein_id="HEO1387199.1"
                     /translation="MNRQKGFSLIEIMVVVVILGILASIVVPKIMGRPDEARKVKAKQ
                     DVLAIQNALDLYKLDNGSYPTTDQGLMALVEKPTSNPVPTNWKQYLKSLPKDPWGREY
                     LYLNPGQHTDVDVFTYGAAGQPGGTGINAEIGNWDDHS"
     gene            complement(3075..4274)
                     /gene="lspF"
                     /locus_tag="VAH76_002607"
                     /gene_synonym="gspF"
     CDS             complement(3075..4274)
                     /gene="lspF"
                     /locus_tag="VAH76_002607"
                     /gene_synonym="gspF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspF family T2SS innner membrane protein variant
                     LspF"
                     /protein_id="HEO1387200.1"
                     /translation="MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQT
                     LTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGV
                     RSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
                     VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSY
                     GLYSLVTMIVALIGFKKSLSNIKIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILF
                     AAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALKETKFISPMAIHLIA
                     SGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIF
                     SMEQLVA"
     gene            4371..5780
                     /gene="glnA"
                     /locus_tag="VAH76_002608"
     CDS             4371..5780
                     /gene="glnA"
                     /locus_tag="VAH76_002608"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO1387201.1"
                     /translation="MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFL
                     ENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYD
                     RCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
                     SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATAN
                     QCEVATRFNTLTKKADELQILKYVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLY
                     KDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLA
                     YSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQP
                     MDKDLYDLPPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEE
                     ITKIRSLTHPYEFELYYSL"
     gene            5793..6353
                     /locus_tag="VAH76_002609"
     CDS             5793..6353
                     /locus_tag="VAH76_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEO1387202.1"
                     /translation="MNKLPLFVFLMMVICLSSAQIYKWTDSQGVVHFSDKPHQGAEKI
                     KIPEAQTYTPPANETGGSSEQEQKSVKQEDQEKYTKLEIIQPQNEATIRNNQGYVVVA
                     VQIEPDLLPGDNLQLIFDGAPLGEPQPNLIFQLNGIYRGSHTIAVQVLNDKGDVLKVS
                     DSITIFMHNPRVGMGKGRKKNINSNK"
     gene            6366..7139
                     /gene="yaaA"
                     /locus_tag="VAH76_002610"
     CDS             6366..7139
                     /gene="yaaA"
                     /locus_tag="VAH76_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO1387203.1"
                     /translation="MLTLLSPAKKLLSISKHYSKETSNPLLLDKALQLVKIMKLKSVE
                     QIADLMDLSRQLAELNYERYQNFDLKNNPMNHSYPALFLFQGDVYQGLNANSWKDEEI
                     EYAQSHLGILSGLYGFLRPLDRIQPYRLEMGVNLENPAGKNLYAFWSKIVTNILNQIL
                     AEQSNPVLINLASTEYFKVVDEKKLSYPIVTINFYEQKNSELKMIGILAKKARGMMAK
                     YIMQNRIDSIEQIKEFSESGYLFNKEISSPNSLNFIRIH"
BASE COUNT         2187 a         1324 c         1422 g         2266 t
ORIGIN      
        1 aatctcagca agaaagacat tttccagaaa ctaaaagcac tcattcctgg taaaaataaa
       61 aatcggatgg taacccatta actaaaagtt ggattctgac gtattcatat tgtttggaac
      121 atttgagctc aagtcaaaaa ttattaatac tctctctaat aacgcttgtt aataacttac
      181 cctttctgtc ccgactgcgt tttaacaaag aatacacaac caaactaaat tcatcattct
      241 ttgcataggc aacgctaaga taatattgac tttcaatggt tatttgatca ctgggaatat
      301 tgaatttttt taataactcg ttgatctctt tcagatcagt gataccattt tccccccgag
      361 caattattaa ttcatttgcc tgggcgtcac ttaatccatc tcctaatgac atcaatattt
      421 gttttggggc agtattaatg ttaatactcg ttgattcagg caaggctgtg ataaaaggtt
      481 caagggcaat ataagcgggt gcacttacat cttttatcaa tcgaaactca gatttacttt
      541 ttataagttg atggctggga tagtaaggag gtttctgaga gagataataa gaggtataaa
      601 tatcttctcc gcggccaaga tcataagcaa gcaaccaatg ctttactccc aaggctatat
      661 tagctcgttc ttggccggtt gcctttgagt aggcgtgact tatcaggtgc attaaagtgg
      721 gaatggattt tttctcaatc aggttattga ggttgaatcg cgcttgtaaa tcaaaaattc
      781 caccacttaa ttgtacttga ttgtatatgg attccatatt tttagggtat tgggctacca
      841 ttcctaactg gttggttttt gtaaagctgt tgttcttatc aagtaattca ttgagagccc
      901 agaaagtaac cgcctgagaa gcaagatata gcttatcctg agcaatcact agccttgttc
      961 tgtaaatgtc ctgttgtaat cgtgtgctca tggcagtggc aactatagca accaatgtca
     1021 taataaatag agccgttaaa agagcgcttc cttgcatttt tgaactggca atggattttg
     1081 gaggtggact actcataaac atagagtgct ccgggtatga taaaaagcat gttcatatct
     1141 ccccagtcct ttaaagtgag attcatttgg attgctttgg gaagaggttc tctttgctga
     1201 ttttgattga cagcctgttc tcgccactcg gagagaagct gtaaattttg atttaaatag
     1261 ttgaaatgac attcagataa attatccaga agtactttgt ctttatagat atttctgtct
     1321 ataggatcta aagtattcca tgtgcggcgc agtaatttgc tatcctggca aacaagtgcg
     1381 actcgttcca aggtactgcg tttttcaaca ctattggggt ttaccacccc atccctgctt
     1441 agttcaaggt attgaggttg accaacaaat attggaaaca ggcgcatttc attacctctt
     1501 atagcacgtt ccactgtttg ggaagtatct tgctgaatga tacttattgc cagctgaagt
     1561 gcatttaagc gttcagcctg cgcgttaact cgggttcgag tattgaatgc gtagtataag
     1621 gttgatgagg taatagttgc taatatagca aaaacagtaa gagcaattaa tatctcaatc
     1681 agtgtaaatc ctttattttt aatcatggta agtacctgaa tgcaacgagt tcttctcgaa
     1741 agggacctgc ttgcttggaa ctgactgaaa tggttatctg ttgcattcgt tttatagggg
     1801 tcgggctaat tttagctctc cagtaccact tttgtcccag catggtggtt gcttgcgttg
     1861 attcttggct ttgagaagtg cggagcagat ttaattggat cattgctacc ccttgcattg
     1921 ctatccaatg actaattgtt ttttctttga ttctatgcgt gttatctgta ttttgagcag
     1981 ttgcttttaa aagcgctgtt aaagcaattg caatgacagt aagagccaat aagacttcta
     2041 tcaaagtaaa tccggagact aatttagttt tcggcatcat tgagattttg ccatggtaaa
     2101 atccaaatca ccattatggc tgcctgtaag tttggctatc catttttcct tatctgtgcc
     2161 aaattctata gtaaaagggg tcatgccacc agatggtgct attatgattg cagggttccc
     2221 ccgggtatgt ttttgatttg ttttaaggct aatcacaacg tttttgggaa aatagtagag
     2281 tttaaaaatt cctttgttcg atatgggatt ccatcccgcc tggtcctgaa acttcagaat
     2341 ttggtaacta gtgttatcta tttttaaccc aagggtgctg ctttctaaaa tggcttgttg
     2401 ttgagccagg cttagggtat ttgccaattg ttcagctgaa aattgtatac gtctgctttc
     2461 cccgaagtca ccaaaagcga ttaaggcaaa ccccaaggtt atacctataa tcactatgac
     2521 aataagaatt tctatcaggg taaaaccctt atgagtggtc atcccagtta ccaatctccg
     2581 cattaatccc tgttccgcca ggttgtcctg ctgctccata agtaaagaca tcaacatcag
     2641 tatgttgccc tgggtttaaa taaagatatt ctctgcccca tggatctttt ggcaatgatt
     2701 tcaagtattg tttccaattt gttggaacag gattgcttgt aggtttttct accagagcca
     2761 ttaaaccttg atcagttgta ggatatgaac cattatcaag tttgtacaaa tcgagtgcat
     2821 tttgaatagc taaaacatct tgtttcgctt tgactttacg ggcttcatcc ggacgtccca
     2881 tgatcttggg gacaactata gatgccaata tgcctaaaat cacgacaaca accataattt
     2941 cgatcaaaga aaagcctttt tgtcgattca tttgtgtacc tcttgaaact aaatgttaaa
     3001 ttataacaca aatattgatg cctatgaata gacttaaatt ttaaaaagac gcggatagcc
     3061 ctttctccgc ttaatcaggc aactaattgt tccattgaaa aaataggtaa taaggtcgct
     3121 agtacaataa ataaaacaac agcacccatt aatagaataa ccataggttc taataaagtt
     3181 aaggatgttt caatcagtcg gtttacttca ttatctaaat gagacgccga gcgttccatc
     3241 atgtctgaaa gttggccgct tttctcacca ctggctatca gatggatagc catggggcta
     3301 atgaattttg tttctttgag ggcctcactg attccacttc cttcacggac tcgtagagta
     3361 gctaaatcaa aagcctgtct catcacgata ttggttacca aacttgaaga aacacgcatg
     3421 gtttcaagta cactgactcc cgctgcaaat agaattccaa acgtgtgaat atatcgcgcg
     3481 acattaattg ttttaaccaa ataagacacg ataggtagtt taagcatgaa ttgatgccat
     3541 gctgttttaa tttttatatt actgaggctc tttttaaagc caattagagc cacaatcatt
     3601 gtcactagac tatacaaacc ataagattta ataaattggc tcaagttaat aagcaattgc
     3661 gtcattggtg gtagtgtttg gccactttcg gtaaatacat caataatttt agggacaaca
     3721 aaagtaagta aaaaactgat aatggcagta gaaacaatga tcatgagcaa tgggtaaatc
     3781 aaagcttgct gcactttctg tcgagtttgt tgttgttttt cagtataatc agccagtttt
     3841 tctaaaacaa catcaagacg ccctgtctgc tcgcctgagc ctactgttgc ccggtatagt
     3901 tctggaaagg cattgggata ttgagccatt gcctgagcaa gaccataccc ttccaatact
     3961 ttagagcgaa ctccgataat aagctctctg actttgtctt tttctgtttg ttcgctgacc
     4021 ccgcgcaatg attcttcaac aggaataccg gcagctagaa gtgttgccag ctgtctggtg
     4081 agtaatgcca aatcagccgc agaaacttta cttttgctgc tggcagcacg ttgctgggtt
     4141 aatgtttgga cttgagtagg gattagtcct tgttctcgca ataattgacg tgcatgacgt
     4201 tcagaatcag cttctattac acctttgcta atgctgccat tcacttttag cgcttgatat
     4261 tggtaggcgc ccatattatt tttgttaaaa ttaaaactat agagtgtaat ctattgggat
     4321 taataagtca cttaaggtaa actatacgcg gactatcctg gagacaaaag atgacaaaaa
     4381 atgtagtgct tgatgcaatc aaagaacatg acgccaaatt tgtggattta agatttacag
     4441 atatccgtgg aaaagagcaa cacattacca tacctgtttc agcagttgat gatgattttc
     4501 ttgagaatgg aaaaatgatc gatggctcat cttttaaagg ttggcagaaa attcatcaat
     4561 cagatttggc tttagtgcca gatttaactt caattaagcc ggatccattt tttcaggaca
     4621 acactttatt tatacgatgt aatgtagttg atccacagac tatgatgggt tatgacagat
     4681 gcccacgttc aatcgcacaa cgcgcagaaa attatttaca atctacaggg attgcagatg
     4741 gtgtaaattt tggccctgag cctgagtttt ttctcttcga caatgtgcaa tgggaaacta
     4801 ctatcagcgg ttcattttat aaaatagatt cagacgaggc acattggaac tccggtaagg
     4861 aaatggaagg cggaaatatt ggccatagac ctccgttgaa aggcggttat ttccctgttc
     4921 ctccagtcga ttcatctcat gatattcgtt cagcaatttg tatcacattg gaatctttag
     4981 gaatggttgt ggaagcacat catcatgaag tagctacagc taaccagtgt gaagtagcta
     5041 cccgttttaa tacactcacc aaaaaggcag acgaactgca aattctgaaa tacgtgatac
     5101 ataatgttgc ccataactat gggaaaaccg ctacttttat gccaaaacct ttggtaggag
     5161 ataatggaag cgggatgcac tgtcatcaat cactttataa agatgggtat aacctgtttt
     5221 ccggtgatca gtattcgggg ctttctgaaa cagctttata ttacataggt ggaataatca
     5281 agcatgccag agctttaaac gcttttacca atccgtcaac caatagctat aagagattag
     5341 ttcccgggtt tgaagcgcct gtattactgg cttactcagc aagaaatcgc tccgccgcta
     5401 ttcgtatccc acacgttagc aatccaaagg cacgacgtat agaagtacgt ttccctgatc
     5461 caacagctaa tccttatctt gctttctcag caatgatgat ggcaggcata gatggcattc
     5521 agaaaaaaat ctaccctggg cagccaatgg ataaagatct ttatgattta ccgcctgaag
     5581 agcttgttga tgtgcccact gtttgctctt cattagagat ggctatggac catttgcggg
     5641 cagatcatga atttttgtta cagggagatg tgttcagcag agactttatt aataattata
     5701 ttcaaatgaa agaagaggaa attactaaaa tcaggagctt gactcatcct tatgaatttg
     5761 agttatatta tagtctctaa gggtctatat tcatgaataa actgccacta ttcgtctttt
     5821 tgatgatggt aatttgcctt tccagcgcgc aaatttacaa atggacggat agtcagggtg
     5881 ttgttcattt tagcgacaaa cctcatcagg gtgctgagaa aataaaaatt cctgaagctc
     5941 aaacctatac accacctgca aatgaaacag gtggttcttc tgagcaagaa caaaagagtg
     6001 tcaaacagga agatcaggaa aaatatacca aattagaaat tattcaaccg caaaatgaag
     6061 caacaattcg taataatcaa ggttatgttg ttgttgcagt ccaaatagaa cctgatttgt
     6121 taccagggga taatttgcag cttatttttg atggggcgcc tttaggagag ccacagccca
     6181 atttgatttt tcaattgaat ggtatctatc gaggatctca tacaattgct gttcaggttc
     6241 ttaatgataa aggggatgtg cttaaagtca gcgacagtat tacaattttc atgcacaatc
     6301 ctcgcgtcgg aatgggcaag ggcagaaaaa aaaatatcaa ttctaataaa tagagcaggt
     6361 tatgtatgtt aactttgtta tcgccagcta agaaactatt atccatatca aaacattact
     6421 ctaaagaaac cagtaatcct ttattgctcg ataaagcgct acaattggtt aaaataatga
     6481 agttaaaatc agtggaacaa attgcggatt taatggattt atcaagacaa ctcgctgaat
     6541 taaactatga gagatatcaa aattttgatc ttaaaaacaa cccaatgaat cactcttatc
     6601 ccgctttgtt tttatttcag ggagatgtgt accaaggatt gaatgctaat tcatggaaag
     6661 atgaagagat cgaatacgct caatcccatt taggaatttt gtcaggacta tatgggtttt
     6721 tacggccttt agataggatt caaccttaca gattggagat gggagttaat ttggaaaatc
     6781 cagcaggtaa gaatctctat gcattttgga gcaaaattgt tactaatatt ttaaaccaaa
     6841 tactggcaga gcagagtaat cctgtactta ttaacctggc ttcgactgag tattttaaag
     6901 tcgtcgatga gaaaaaattg agttatccta ttgtgactat caatttttat gaacagaaaa
     6961 atagcgaatt aaaaatgata ggtatccttg ctaaaaaagc aagaggaatg atggcaaagt
     7021 atataatgca gaatagaata gactccatag aacaaattaa agagttttct gagtcgggtt
     7081 atttatttaa taaagaaatt tccagtccaa atagtttgaa ttttattcga atccattaaa
     7141 ttatttaaaa ggtatttgat gtggaatttg tccagtcggg tgcatgccct taatcattt
//
LOCUS       DATNSN010000157         7130 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.157, whole genome shotgun sequence.
ACCESSION   DATNSN010000157 DATNSN010000000
VERSION     DATNSN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.157"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            45..995
                     /locus_tag="VAH76_002611"
     CDS             45..995
                     /locus_tag="VAH76_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HEO1387204.1"
                     /translation="MQPKFELFDELPLEIQIKTAKKLSNPDLVSFSMRSKDHFALFKP
                     MVDVNKLLYYVTRGNHEAVKAILKEDMSLMFKRGKVKDCSGRTFHDVSSFEYALWALD
                     KHMWALMLACIPQNEEGQIVFAKLLIQYSKINTDGITYKLNGETVTEKHFDFKNTIIK
                     ELQTQLDLLNAPGTKNWDTIDKQWREGLGGAQKLLPVHVVDEYCSNEPFSPIPKFTAR
                     PKSSRQFYNSMTGKHENWFDLNSKLGSGFAIYKGVSVAPNAEEYGHVARCCLWARVGD
                     LPAIEALCEARTKDFINLKSQLDKHMTVDSKPQAHKIRPI"
     gene            1223..2125
                     /locus_tag="VAH76_002612"
     CDS             1223..2125
                     /locus_tag="VAH76_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEO1387205.1"
                     /translation="MIKQLQQALSDILLGKEEVIRLGITCLLANGHLLLEDNPGMGKT
                     TFSHALAQLTGMQYRRIQFTSDLLPSDLLGVSIYDHQRNVFQFHKGPIFSQLILADEI
                     NRATPKTQSALLEAMEERQVTTDIGTEALPQPFFVIATQNPSYHTGTYPLPESQLDRF
                     LMRLVIGYPPPDAERMLLKSSNLNAQLKELSPIINPQQLQILQRESQKVLVSELVLDY
                     VQSLLDASRNQGWFNHGLSPRAGMGLVHAARAFARTDDRDFVRPDDIQAVMPAVVNHR
                     LIIKKSQTQLSAAELLMSEIEVPV"
     gene            2122..3072
                     /locus_tag="VAH76_002613"
     CDS             2122..3072
                     /locus_tag="VAH76_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387206.1"
                     /translation="MIRQIKIKVMAYWEEWIAKRISKTNPQILGSQNIYIMPSGFGWA
                     YGVVVLSLLSGAINYQVSTVFLMSFILAIVGFISAWEAHANLKGLSIKLVSVEDTYEG
                     TPAQVSLLIQSNNRMRFGLDFRLNNQSVTRLEKIPPQGLRFILPLATTRRGCYTLPKI
                     TISSFFPFGLFQVWGYANFDSNYYVYPQPVNPGFWPTPFYHQNKKKTDSQGDNEYYDL
                     KQVDNPWIQPNLIAWKIAAKGQGWYLKTMDRAEEMYWLFRLNDLPIETLEEKLKHMSY
                     WLVTAESQGQFYKLELGNDPDKFSHGEEHLRYCLRQLAVY"
     gene            3072..5060
                     /locus_tag="VAH76_002614"
     CDS             3072..5060
                     /locus_tag="VAH76_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing transglutaminase
                     family protein"
                     /protein_id="HEO1387207.1"
                     /translation="MDFFSKQHIFLITTRYALLVMLICYFPHFLTCPLWLSVLILATL
                     CYKLISDYFAYPLLDGKIRFIVVIICLFLLKFQYGSIISSGFFIGFLLAFIGLKTIEI
                     HNDRDIRILILCNFYLIFAALIVVQELWIIPYLLIAILANFTLMLKLTAPQASLRQIS
                     GRSMKLLLIATPLTVFLFYFFPRIANPLWQVPSLGKSHTGFSEWMEPGSVANLLNDDR
                     TALRIIFKRKAILNGYWRGLTLSRYNGISWNPAWHDSSTFSPLKELTTSDDGDYEVIM
                     EPHQKKWLFYLGYPNAAYPQLLFSPNFGLVSHNKDMIAQRFAYALRISSSPHSSLTQK
                     DWKLNTQLPKYSNPRITFWAKKQFAEVNYDPQAFIQFIQNYIKRESFWYTLTPPKLDN
                     ENDQIDYFWFDSKKGFCEHYASAVTVVLRAVGIPARVIIGYQGGEWNPLAKYLTIKQY
                     DAHAWVEYWQKDFGWKQLDPTAFISTSRIDPSVLAMRAARTNQQLIIDPSNLNWIQKT
                     KLYMESTRFFIERWLLFYNQDAQYKLLEQIGLKHWNAAMLLQIAILSLVLFIILLGAC
                     YQWRQKRTLDPLLREYHLLQKEFCRFDIATQPPATLNKQCKILINKIPNLEETITTFL
                     SHYEKLRLRHRITKTKENKKQTLLLFKNLRRILKQVKI"
     gene            5142..6326
                     /locus_tag="VAH76_002615"
     CDS             5142..6326
                     /locus_tag="VAH76_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO1387208.1"
                     /translation="MVHFQKMTVQTFLKDYWQKKPLIIRQALPDFTNPLTPDELAGLA
                     LEEEIESRLVYETPDHSPQWNLKRGPFKESDLIGLPKTHWTLLVQGVDRIVPDVYELL
                     EHFNFIPQWRVDDVMISYATLHGSVGPHYDNYDVFLYQAKGRRLWSLTSKKCHTNNFI
                     KGLELRIMKEFEVEEQFILEEGDMLYLPPHIGHYGIAQSEECMTYSFGYRSYQGQELW
                     DSLGDYLSEHGLFKSLYQDPDWSTLKSTSEITPKAWDNARQLLRQVLENDKLMQSWFG
                     CFATSLDQSAEQYLPPPLEEDELLGLDEFIKELANYQEIVRDASCRFAYIMSDQESQC
                     RFYVNGKEWDSRGVSTNLLSLVANNRFLSLKELKPYLSHKTNQLFLYELWKLQWLQIS
                     HD"
     gene            complement(6444..6737)
                     /locus_tag="VAH76_002616"
     CDS             complement(6444..6737)
                     /locus_tag="VAH76_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO1387209.1"
                     /translation="MPVKQPAIYIMANKRNGTIYTGVTSDLIKRVYEHKYGDVPGFTQ
                     EHGCKFLVYYELIEDMISAIAREKQLKGGSRKKKLALIEKLNPYWEDLYEKLL"
     gene            complement(6763..7041)
                     /locus_tag="VAH76_002617"
                     /pseudo
     CDS             complement(6763..7041)
                     /locus_tag="VAH76_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2184 a         1400 c         1341 g         2205 t
ORIGIN      
        1 tttctttccc aaattaattt aagtgaagaa aataaggtga aagtatgcaa cctaaatttg
       61 aattgtttga tgagttacct ttggaaatcc aaatcaaaac agcaaaaaaa ttatcaaacc
      121 ccgatttagt aagcttttct atgagatcaa aagatcattt cgctttgttt aaacccatgg
      181 ttgatgttaa taaattattg tattatgtga cgcgcggaaa ccatgaagcg gttaaagcga
      241 tattaaagga agacatgagt ttaatgttta aaaggggcaa ggtaaaggat tgctccgggc
      301 gaacatttca tgacgtcagc agctttgagt acgcgctttg ggccttggat aaacacatgt
      361 gggcattgat gcttgcgtgt ataccgcaga atgaagaagg ccagatagtg ttcgcaaaat
      421 tacttattca atacagcaaa atcaacacag atggtataac ttataaactt aatggggaaa
      481 cagtcactga aaaacatttt gattttaaaa ataccatcat caaagagttg caaacccaac
      541 tggacttgct aaacgcacca gggacaaaga attgggatac cattgataag caatggcgag
      601 aaggtcttgg cggcgcacaa aagctgctgc ctgtgcatgt tgttgatgag tactgctcca
      661 atgaaccttt ttctccgata ccaaaattta ccgctcgacc gaaatcatca aggcaatttt
      721 ataattctat gactggcaag catgaaaatt ggttcgatct taattctaag ttaggctctg
      781 gttttgcaat atataagggg gtatctgtag ctccgaatgc agaggagtac gggcatgtgg
      841 cacggtgttg tttgtgggct cgggtgggtg atttgcctgc cattgaggcg ttatgtgaag
      901 caagaacaaa ggattttatc aatctcaaat cacaacttga taagcatatg actgttgata
      961 gtaaacctca agcccataaa atacgaccta tttgataata ttttgatgca agtgtaaacc
     1021 ctaaaatcac ccatcaagtg agtctttgta tcttggctaa ggctctattt gatgagcagt
     1081 ttgattggtt cagaacttcc tcgattaaat ggctattccg cgcaattgac ttgttatttg
     1141 tagtaaattt tacattaatt cgaggtcata cccatcataa aggtctatac ttccatataa
     1201 tcatcttgaa atggctttca ttatgataaa acaattacaa caggcattaa gtgatattct
     1261 tttaggaaaa gaagaagtca tcagattggg tattacctgt ttattagcca atgggcacct
     1321 gcttcttgaa gacaacccag gtatggggaa aacaacattt agccatgcgt tggctcaact
     1381 gactggtatg caatacaggc gaatacaatt taccagcgac cttttgccca gtgatctctt
     1441 gggagtatct atttatgatc accaacgcaa cgtctttcaa tttcataagg gtcctatatt
     1501 ctctcaatta attctcgctg acgaaataaa cagggccaca cctaagactc aaagcgcctt
     1561 attagaggca atggaagaga ggcaagttac tactgatata ggcacagaag ctcttcccca
     1621 accatttttt gttattgcta ctcaaaatcc gtcttaccat actggaactt atcctttgcc
     1681 tgaatcgcaa ttggatcgat ttctgatgcg cctggtcatt ggctacccac cgccagatgc
     1741 ggaacgtatg cttttaaaat catcaaactt gaatgctcaa ttaaaggagc taagccctat
     1801 cattaatcca cagcaattac aaattttaca aagagagagt caaaaagttc tagtctctga
     1861 attggttctt gattatgttc aatctctgct ggacgccagt agaaaccagg gatggtttaa
     1921 tcatggatta agcccaagag caggaatggg gttggtgcac gcagctcgag cctttgccag
     1981 aactgatgat agagattttg ttcgacctga tgatattcaa gcagtcatgc cggctgttgt
     2041 gaatcaccgc ttaatcataa aaaaatcaca aactcaactc tctgccgctg aattattaat
     2101 gagtgaaatt gaggttcccg tgtgataagg caaatcaaaa taaaagttat ggcttattgg
     2161 gaagaatgga ttgccaaacg aatctctaaa accaatcccc aaattcttgg ttcacaaaat
     2221 atttatatta tgccctctgg atttggatgg gcctatggtg tggttgtact gtccctgctt
     2281 tctggcgcaa tcaactacca agttagcact gtttttttaa tgagtttcat tttagcaatt
     2341 gtaggtttta taagtgcttg ggaagctcat gccaatctta aaggattatc tattaaattg
     2401 gtatcggttg aggataccta tgaaggaacg ccagcccagg ttagcttatt gattcaatca
     2461 aataatagaa tgcgttttgg actagatttt cgccttaata atcaatcagt cactcgatta
     2521 gaaaaaattc ctccgcaagg tttgcgattt attttacctc tggcaacaac tcgaagagga
     2581 tgttataccc tgcccaaaat caccatttcc agtttttttc cttttggctt gtttcaggta
     2641 tggggatacg ccaattttga tagcaattat tatgtctatc ctcaacctgt caatcctggc
     2701 ttttggccca ctccctttta tcatcagaat aaaaagaaaa cagactctca aggagataat
     2761 gaatactatg acttaaagca ggtagacaat ccctggattc aacctaatct tattgcatgg
     2821 aaaattgcag caaaaggaca aggttggtat ttgaaaacga tggaccgcgc tgaagaaatg
     2881 tattggcttt ttagattaaa tgatttacct attgaaacac ttgaagaaaa attaaaacac
     2941 atgagctatt ggttggttac tgccgaatca caaggccagt tttacaaact cgagttagga
     3001 aacgatccag ataaattttc ccatggggaa gaacatcttc gatattgctt acgtcaattg
     3061 gcggtgtatt gatggacttt ttttccaaac aacatatttt tctgataaca acccgatacg
     3121 ctcttctggt catgttgata tgctactttc ctcatttttt aacgtgccct ttgtggctta
     3181 gtgtcctcat cttggcaacg ctatgttaca agttaatatc cgattacttt gcctatccac
     3241 ttcttgatgg caagatacgc tttattgtgg ttatcatctg tctttttctt ttaaaatttc
     3301 aatacggtag cattatctca agtggttttt ttattggttt tttactggca tttattggat
     3361 taaaaactat tgaaattcat aatgacagag atataagaat actaatactg tgcaattttt
     3421 atcttatctt tgccgcatta attgtggttc aggaactatg gatcatacct tatttattaa
     3481 tagcaattct ggctaatttc actctgatgt taaaattaac tgctcctcaa gcctcactaa
     3541 gacaaattag cggccgtagt atgaaactat tactgattgc aacaccatta actgtctttc
     3601 ttttctactt tttcccccgc atagccaatc ccctttggca agtgccgtcc cttgggaaaa
     3661 gtcacactgg atttagcgaa tggatggaac cagggtcggt cgcaaatctt ctaaatgatg
     3721 acaggactgc tttacgaatt atatttaaaa gaaaagccat tctaaatggc tattggcgag
     3781 gattaacttt aagtagatac aatgggataa gctggaatcc tgcctggcat gactcatcaa
     3841 ccttctctcc attaaaggaa ttaactactt ctgatgacgg ggattatgag gtcattatgg
     3901 agccccacca aaaaaagtgg ttgttttatc ttggttaccc caatgcggct taccctcaac
     3961 tcttattttc tccgaatttt ggtttggtta gtcataataa agacatgatt gcccaacgtt
     4021 ttgcctatgc tttgcgaatt agctcttctc ctcactcctc attgacgcag aaagattgga
     4081 aacttaatac tcaattacct aaatattcaa atcccagaat tacgttctgg gcaaaaaaac
     4141 aatttgccga agtaaattat gacccccaag cctttattca attcatccag aattacataa
     4201 aaagggaatc cttctggtat accctaactc ctccaaaact ggataatgaa aatgaccaaa
     4261 tagattattt ctggtttgat tccaaaaaag gattttgtga gcattatgca agcgcagtca
     4321 ctgttgtatt acgcgctgtt ggtatccctg cccgggttat tattggctat caaggaggtg
     4381 aatggaatcc actggcaaaa tatttaacta taaaacaata tgatgcacat gcctgggtag
     4441 agtattggca aaaagatttc ggctggaagc aactggatcc cactgcattt atttccacct
     4501 cacgtatcga tccttctgtt cttgccatgc gagcggcgcg caccaatcaa caattaataa
     4561 ttgatccctc aaacttaaac tggattcaga aaaccaaatt atatatggag tcaacccgtt
     4621 tttttatcga gcgttggctg ttattttaca atcaggatgc tcagtataaa ttattagaac
     4681 aaatcggatt aaaacattgg aatgccgcca tgctattgca aattgccata ttgtctctgg
     4741 ttttatttat cattctcttg ggtgcctgtt atcaatggcg gcaaaaaagg acattagacc
     4801 ccttattgcg tgaataccac cttttacaaa aagagttttg tcgcttcgat attgcaactc
     4861 aaccacccgc cacgctgaat aaacagtgta aaatattaat caataaaatt cccaatctgg
     4921 aagaaaccat cactaccttt ctttctcact atgaaaaact gcgtttgcga catcgcatta
     4981 ccaaaactaa agaaaataaa aaacaaacgc ttttgttgtt taaaaatctg cggcgaatat
     5041 taaaacaggt aaaaatctaa atttgacggg atttatcggt tgcattcttg agtctcaaag
     5101 tgcttaaata gataacattt atcaaactat gatttttaat aatggttcac tttcaaaaaa
     5161 tgactgtcca aaccttttta aaagactatt ggcaaaaaaa accattgatc attcgtcagg
     5221 ctttacctga ttttactaat ccactcacac cggatgagtt ggcaggactt gcccttgagg
     5281 aagaaataga aagtcgtctg gtctatgaaa cgcccgatca ttccccgcag tggaatttaa
     5341 aaagaggccc ttttaaggaa tctgatttga taggcttgcc taaaactcac tggactctat
     5401 tagtacaagg agtagaccgt attgttccag atgtctatga gttacttgag catttcaatt
     5461 ttatccctca atggcgagtt gatgacgtga tgattagcta tgccactcta catggaagtg
     5521 tgggcccaca ttacgataat tatgatgttt tcttgtatca ggctaaaggt cggcgactat
     5581 ggtcattaac ctcaaaaaaa tgccacacca acaattttat aaaaggtctt gaactacgaa
     5641 tcatgaaaga atttgaggta gaagagcaat ttatcctgga agaaggtgac atgctctatt
     5701 taccccccca tataggacat tatggcatcg ctcaatctga agaatgtatg acttactctt
     5761 ttggttatag aagttaccaa ggacaagagt tatgggatag tctgggtgat tatttgtctg
     5821 aacatggctt gttcaaatcc ctctatcaag acccggattg gtccactctt aaaagcacat
     5881 cggaaataac gcccaaagca tgggacaatg caagacaatt gctaagacaa gtcctggaaa
     5941 atgataagct tatgcaatcc tggtttggtt gttttgctac aagccttgat caatcagcag
     6001 aacaatattt accccctcct ttagaagaag atgagttact cggtttggac gaatttatta
     6061 aagaattggc taattatcag gaaatagtta gagatgcctc atgtcgtttt gcttatatca
     6121 tgtctgacca agaatcccag tgccgttttt atgtcaatgg gaaagaatgg gacagccgtg
     6181 gggtttcaac aaacttactc agtcttgttg ccaataatcg attcctgtct ctaaaagagt
     6241 taaaacctta tttaagccat aagactaatc aactcttcct ctatgaatta tggaaattac
     6301 aatggttaca aatttctcat gattaatgat ttggtttata aatatcttga ataaaattca
     6361 cctctttacg gtacgatttt tgggaccgtc gttgcgagga gcgtagcgac gaagcaatcc
     6421 agtgacctta aaattgctaa tcttcacaac agtttttcgt aaagatcttc ccaataaggg
     6481 tttagctttt caattaaagc caatttcttt tttcttgacc cgcctttaag ttgtttttct
     6541 cgagctatgg cacttatcat gtcttcaatg agctcatagt aaacaagaaa cttacatcca
     6601 tgctcttgag taaacccggg tacatcccca tatttatgtt catagacacg tttgatcaaa
     6661 tcgcttgtga cacccgtgta gatcgttcca ttgcgtttat tcgccatgat gtaaatggct
     6721 ggttgtttca ctggcatgct tcttccttgt tattggacca attcattcag cagcgcatat
     6781 cacgcgacta atgcacaaaa acccaagtat atcggagaaa gaatggtgaa taaactgaca
     6841 gccattaaac gcagcatcac gtaataaatc aaatccataa cgaaaaaaac ttaccccttt
     6901 gcgaccatgt tttttgattt taattgcctt ctgaacatgc cgccactctc cagttttatg
     6961 ggcccaacaa aaagcaatgg tcaatagcac cagtaatttt tcaatgcgat ccggttgagt
     7021 catgtgtgtg tcttcaaaat cgtttgattc ttgtgtacct tgaatcttgt ttagcagggc
     7081 tagaaaaacc acatgcaatc tcagttaaat tgaccgttct tactttgatt
//
LOCUS       DATNSN010000158         7090 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.158, whole genome shotgun sequence.
ACCESSION   DATNSN010000158 DATNSN010000000
VERSION     DATNSN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.158"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            13..705
                     /gene="ubiG"
                     /locus_tag="VAH76_002618"
     CDS             13..705
                     /gene="ubiG"
                     /locus_tag="VAH76_002618"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEO1387210.1"
                     /translation="MNQIESTIAIEEVHKFAQLANDWWDTNGPLRTLHDINGARFEFI
                     SEHINLKGLRVLDVGCGGGILCESMAKAGAYVSGLDAEPEAIQIAKEHAHKNQLQIDY
                     FCNPIEEYEEQGFDVITCMELLEHVQNPELVLQHCRRLLKPNGLLFLSTISRTLKAYL
                     GAIIAAEYVLNLLPRQTHDYDKFIKPSELVKMARLYDLNLIDMKGLCYNPFLRKTTLV
                     SDVSINYIMVLQ"
     gene            complement(733..1098)
                     /locus_tag="VAH76_002619"
     CDS             complement(733..1098)
                     /locus_tag="VAH76_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HEO1387211.1"
                     /translation="MNQQLNATKKDKLQVIDWLIENFPNAFFKKGNQVKPLKIGIFDD
                     LIDFYERLDTPPFSKKSLREALSYYSASPAYLSCQKPDTARVDIYGNEVDVVTPEQAK
                     YAYQRYQERYGNKKSQELK"
     gene            complement(1530..2207)
                     /locus_tag="VAH76_002620"
     CDS             complement(1530..2207)
                     /locus_tag="VAH76_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEO1387212.1"
                     /translation="MNTLFTLISTVLLTSNITSSTPLFAKPGTDINTQVQHLSHKAPQ
                     LNKKVLKLALTAYKNASKRGAVKKPVLTVIDYSLPSNKQRMWVFDLRNERLLYNTYVA
                     HGKNSGVNVARSFSNRESSKQSSLGTYITKNTYIGHKGYSLNLQGLDRGFNDNAYNRR
                     VVIHGAWYVEPEFIKKAGRAGLSWGCPAIAQTLAKPVINTIKNGSVVFAYYPDKKFLS
                     HSGYLVA"
     gene            2764..5916
                     /gene="putA"
                     /locus_tag="VAH76_002621"
     CDS             2764..5916
                     /gene="putA"
                     /locus_tag="VAH76_002621"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEO1387213.1"
                     /translation="MLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMM
                     AIKTSATKLVQSVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDN
                     LIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSS
                     EAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAA
                     RYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLA
                     LSRLAKDYGIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYV
                     LDWVAALARSKQRRIMVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAK
                     KILTMTDAIYPQFATHNAYSVAMILNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGI
                     PCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKAPISELVEDPVAKSRSLLD
                     KINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMIAGRKLS
                     RDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFELWSKKPVEERASCLNRFA
                     DLLQANMAELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFNGYTG
                     ELNELSLHPRGTILCISPWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKL
                     MHQAGIPEGVIQLIPGAGETIGAALVADKRIKAVLFTGSTDTANLINRTLATRGGEII
                     PLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSALRVLYVQEEVYPRTVEL
                     LKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDEALSG
                     YFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTSYGLTLGIHSRIN
                     ETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTY
                     TVDTTAAGGNASLMSIPEEG"
     gene            6082..6646
                     /locus_tag="VAH76_002622"
                     /pseudo
     CDS             6082..6646
                     /locus_tag="VAH76_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014609561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6848..7057
                     /locus_tag="VAH76_002623"
     CDS             6848..7057
                     /locus_tag="VAH76_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387214.1"
                     /translation="MHQWVSWWTRTANDWTKHNLLTWFIDTCWDASAKEVARMLLHRL
                     DGAQAALANSDHSYVNQALVLVQAQ"
BASE COUNT         2194 a         1216 c         1507 g         2173 t
ORIGIN      
        1 atttattgtc ttatgaatca aattgaatcg actattgcta ttgaagaagt acataaattt
       61 gcccaacttg caaatgattg gtgggatact aatggtcctc tcagaacttt gcatgatatc
      121 aatggcgctc gttttgagtt tatttcagag cacattaatc taaaggggct gagggtgttg
      181 gatgtcggtt gcggtggcgg gatactctgt gaatctatgg ccaaggcagg agcttatgtg
      241 agtggattag atgcagaacc agaagctatt caaattgcca aagaacatgc tcataaaaac
      301 cagttgcaga ttgattattt ttgcaatcct attgaagagt atgaggagca aggttttgat
      361 gtaataactt gtatggagtt attggaacac gtacaaaacc ctgagttggt tttacaacat
      421 tgcaggcgtt tattaaaacc aaatgggcta ttatttttat caactattag cagaacgtta
      481 aaagcttatc tcggcgctat tattgctgcg gagtatgtgc ttaatttgtt acccaggcaa
      541 acacacgatt atgataaatt cattaaaccc agtgagttag ttaaaatggc acgcctttac
      601 gatttaaatc tcattgatat gaaaggccta tgctataatc ctttccttag gaagaccact
      661 ctggtttctg atgtaagcat taattacatt atggtcctac aataaaacaa atccaaatgt
      721 ggcttttccg cgctatttta actcttggct ttttttattg ccatagcgct cctgataacg
      781 ctgataagca tacttggcct gctctggcgt aactacatcg acttcattac cataaatatc
      841 tacacgtgct gtatcaggtt tttgacagct taagtaagct ggagaagcac tgtaatagct
      901 taatgcttct cttaacgatt ttttactaaa aggcggagta tccagtcgct cataaaaatc
      961 aattagatca tcaaatattc caattttgag cggtttgacc tggtttcctt ttttaaaaaa
     1021 tgcattagga aaattttcaa taagccaatc gattacttga agcttatctt tcttggttgc
     1081 atttagttgt tggttcataa tcataccccg ctacatttca tcgtaaataa actaccatat
     1141 cttatgacat aatcaagcag catattgctc taaaagataa aatgacgtaa tttaagctgt
     1201 aattgatgta agaaatttct caattccata gaagaaatta ttctgtttac ctggcaataa
     1261 ttcttacaca agtaggtaag ttattgatta ttaataaaat aaatatataa gactatttct
     1321 gatctgaaaa tagtattgag tgaaggtgtg atttaacacc ttgcgcttcc cctattcaat
     1381 tgctaaattt taatcaagac ttatatggat atgagtcgtg caaatggatt tgcaattaga
     1441 tggatctgat ttgtcaggat gacaataaga acgcaagttc ttatacggat gactgcgaaa
     1501 agccagggct gagtccctgg cttacttttt tatgctacaa gataacctga gtgagataaa
     1561 aactttttat cagggtaata agcaaaaaca accgaaccat ttttaatggt atttatcacc
     1621 ggttttgcca aggtttgtgc tatagctggg cagccccagg ataaaccagc gcgacctgcc
     1681 tttttaataa attcaggttc aacataccac gcgccatgta tcacaacacg tctattgtat
     1741 gcattatcat taaagcctct gtccaggcct tggaggttca aggagtagcc tttgtgtccg
     1801 atataggtgt tttttgtgat ataggtacct aagctggatt gtttgctgga ctctctgtta
     1861 gaaaaactcc gtgcaacatt aactcccgaa tttttaccat gggctacgta cgtattgtat
     1921 aatagacgtt cattacgaag atcgaatacc cacatacgtt gcttatttga aggcaaggaa
     1981 taatcaatta ctgtgaggac aggcttttta actgctcctc tcttactcgc atttttatag
     2041 gccgttaaag caagctttaa tactttcttg tttaattgag gggccttgtg gctaagatgt
     2101 tgtacctggg tgttaatgtc tgttccaggc ttggcaaata atggagtgct ggatgtgata
     2161 ttacttgtta gtagtacagt tgagattaga gtaaataatg tattcatact gctcctttct
     2221 tcttgttaaa tcaccattat tggttattac aaaagatggt gattatatta acaaataagt
     2281 taattgtaaa tttataaggg tttaagttgc ccattaggta aaaatattaa tcatatcatg
     2341 aggttaatta caggctgaaa aagagaatgg agcaaaatgt tttcaataag ttcactaatt
     2401 gctctatatg tataaccaaa aaaacatctt attgattata attagatctt gttggttaaa
     2461 tttagttcta tttttctggg ttaaaaagca tgtttttact ttatcgaaca ggattgtcgt
     2521 catataatat gccacaaaaa gggggttgat gcattgattt tatatagccc aatttattgc
     2581 tcgatactat gatttatatc tgaaatattg ccttgtcgta gagaggggca tggcaaataa
     2641 gccagtgtaa gatatcataa ccggattttt tcattgttat aataatgaat ttaatttttg
     2701 tcttatatgg taaaaattta ataactattt ttttcaatta ccactttagt cttggagaac
     2761 attatgctgg aaaagcagtc cattcattta ccggaaggat taagagctgc tattaataaa
     2821 gcttatcgca tggatgagct ttctttaatt acagagctta gtgaacaggc agcacttgat
     2881 cctcagcaaa tgatggcaat taaaaccagt gctacaaaat tggttcaatc agttcgtagt
     2941 gagagaaaga aaagcacagg aatagattct tttttaacgg agtatgcctt atcaagtgat
     3001 gagggaattg ccttaatgtg tctggcagaa gctttattac gcgttcctga taatgccacc
     3061 atagataact tgattaaaga taaattagct ggcggtgatt ggggggcgca tcgtggacag
     3121 agtgaatcat tttttgtaaa tgctactact tgggctttaa tgctaactgg taaagtttta
     3181 actcctgaaa aagctgaaaa tacattaacc aaagctttac taaaattggt taatcgaagt
     3241 agtgaggctg ttgttagaaa agcagtggat aaagccatgc gaattatgag caaacaattt
     3301 gtgatgggac gtaccattaa tgaagcgtta gctcgagcga aaaaaaagga agacagggga
     3361 tacagatatt cttacgatat gcttggtgaa gctgccttaa catctgctga tgctgcaaga
     3421 tattttgaag cctacaagga agcaatcatt tcaattggag agaaagctga taaacattca
     3481 gatgtatata gacgccctgg tatttccatc aaactttctg ccttacatcc acgatatagc
     3541 gagtttcagt atgagcgtgt catggcagaa ttacccccca aattattagc cttgtctcgt
     3601 ttagcaaaag attatggtat tgctttgaca attgacgccg aggagtcaga gcgtttagat
     3661 ctttcgcttg atgtgataga gaaagtgttt actgatgaga gtttacaggg atggaatggg
     3721 ttcggtttgg ccgttcaatc ctatcaaaaa agagcttttt atgtcttgga ttgggttgct
     3781 gctttagcaa gaagtaagca gcggcgtatt atggtcagat tgattaaagg agcctattgg
     3841 gatagtgaaa tcaaaaaaac gcaaatgcaa ggcttttctg aatacccggt ttttacccgt
     3901 aaagtattta ctgacgtgtc atttcaggct tgtgcaaaaa aaatcctgac tatgaccgat
     3961 gccatttatc cacaatttgc cacacataat gcttattctg tagcaatgat tcttaatttg
     4021 gtaggaggtt atagagattt tgaatttcaa tgtctgcatg gtatgggtaa tgaactttat
     4081 gaacagattg ttccagcaaa ttgttatggt attccatgcc gtatttatgc accagttggt
     4141 agccatgagg atttattacc gtatttagtt cgacgattac tggaaaatgg agcaaactca
     4201 tcttttgtca atcgcatagt tgatgataag gcacctatta gcgagttagt ggaggacccg
     4261 gtagctaaat caagatcgtt acttgataaa ataaataaaa atataccatt gccagaggat
     4321 atctttttac ccgtacgaaa aaattctaaa ggctttgact ttacaaatcg acttgaacgc
     4381 gcattattgc agcaggaact ggcaaaaatt gaatcaaaag aatggcaggc aagtcctatg
     4441 attgctggtc gaaaattatc cagggactta ctgcaaacag ttatgtcccc acagcagccg
     4501 gcatatgcta ttggctcagt gcagcaagcc acacttgatg atgtagaggt ggcattaaat
     4561 caggcaaaat tggcttttga attgtggagc aagaaaccag tagaagaacg cgcttcttgt
     4621 ctaaatcgat ttgcagattt attacaggcc aatatggcag agctcatggt attgacttgt
     4681 cgggaagctg gtaaaacctg gagtgatggc attgctgaag taagagaggc tattgatttt
     4741 tgtcgatact atgccaagaa agctcaagag cttatgagta gccctcaacg ctttaatgga
     4801 tacactgggg aactaaatga attgagtttg catcctcgcg ggacaatatt gtgtattagt
     4861 ccctggaatt ttccattggc tatatttacg ggacaagtcg tagccgggct ggttacaggc
     4921 aattgtgtta ttgcgaaacc ggcagaacaa acgccactaa ttgcagccta tgctgtgaag
     4981 ttaatgcatc aggccggtat ccctgaagga gtaattcaat taattccagg cgctggagag
     5041 actattggcg ctgctttggt cgcagataaa cgaattaaag cagtattatt tacaggctcg
     5101 actgacacgg cgaatttgat taatcggacg ctagctactc gaggtggtga aatcattcct
     5161 ctaattgctg aaacaggtgg tcaaaatgca atgattgtcg attcttcagc attattggaa
     5221 caagtggttg ttgatgctgt cacttctgcc tttggcagcg cggggcagcg atgctcggca
     5281 cttagggtat tgtatgtgca agaggaagtg tatccaagaa ctgtagagct attaaaaggt
     5341 gccatggctg agctagtcgt aggggatcct caatggttat caaccgatgt tggccctgtt
     5401 atagacaagg aagcattatc tatattgaaa aatcatgtcg aaaacatgag aaaacatcat
     5461 gaaatattat atcaatgcac tgttgacgat gaagctttat caggatattt catgccgcca
     5521 acagctattg caattgatag tatcagcgct ttggaaaaag aagtatttgg gccaattttg
     5581 catgtgattc agtttaagag aaaagatctg gataaggtaa ttaatcaaat caatcaaact
     5641 agctatggtt taacactggg aatacacagc cgaattaatg aaacagttga ctacattagg
     5701 caaagggttc atgcagggaa ctgttatgta aatcgtaata tgattggcgc agtagtaggt
     5761 ctacagcctt ttggtggtga agggttatct ggcacaggac ccaaggctgg aggccctaac
     5821 tatttgattc gactttgcca tgagcgtacc tatacagttg atacaactgc tgcaggaggc
     5881 aatgccagtt taatgtctat cccggaagaa ggttagcagt attagaaatt cacttaagag
     5941 aataataaaa tcttaagtga atttattaca ggagatctgc ccctgtctgg aacggcaata
     6001 tttggttgtt cttgtatgag ggctgatcta taccgctgat ctatgaaaat gcttgctttt
     6061 ttgatctgag aagatcatgc tatgccgcat gaaaagaata cacttatcac cagaagaaaa
     6121 gaagggctgg aagcaaggaa tagttgttgc tctaacggta aggagcgtga tcgtataaaa
     6181 gctgttttat tgcgttatat tattgcctat gtagagaaga aatggtctat tatctatagt
     6241 gttccaggga tgaataaatg gatgcatcgt aatggcttta gctacaagaa acctaaaggt
     6301 catccacata aagcgaatag agaacagcag gagcagtctg ataagagcaa accatggttc
     6361 aaaaggaatc attcatttaa tacttgacca agccccttat catcgagcag ataaagtcaa
     6421 agaaaaggct aaaaaattag ttattcactt aaactatctt cctccttata gtcccaattt
     6481 aaaccctatc gaacgattat ggaaagtcat gaatgaaaag gtcagaaaca atcgattttt
     6541 caacagtgct aaggatttta gaagagaaat tgaacggttt tttactgaaa tactgcctga
     6601 tatcggtgca tcattaggac aaagtatcaa tgataatttc cagtgacttt gacattggag
     6661 catcatgaaa cgctgggatt ttgtagagat gacgaatacc ggtagcaagc tttttgcaag
     6721 cacccataag cgcaagaagt ttgcccatca caaacttaat ggctcgtact atcagtgaat
     6781 ggttaactca aggggtgcaa tccattatca atggaaccta tacacctccc ttcgagccat
     6841 gagttacttg catcaatggg tctcctggtg gacgcgaaca gcaaatgatt ggacaaagca
     6901 taacttatta acctggttta ttgacacctg ttgggacgca tccgcgaaag aagttgcaag
     6961 gatgttgctt caccgcctcg atggagcaca agcggcttta gctaactcgg accactcgta
     7021 cgtaaatcag gcacttgttc ttgttcaggc tcaataatat ttgcttttgc gtcttcaaca
     7081 agccgccata
//
LOCUS       DATNSN010000159         6753 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.159, whole genome shotgun sequence.
ACCESSION   DATNSN010000159 DATNSN010000000
VERSION     DATNSN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.159"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..230
                     /locus_tag="VAH76_002624"
     CDS             <1..230
                     /locus_tag="VAH76_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEO1387215.1"
                     /translation="ACNKNILPFKFNVDTATFMVEIPNIMASKEKRSLSFYWQEKLFA
                     FLMRNYSANLNIEFYKLPYNRTIAIGTYCIL"
     gene            348..1286
                     /locus_tag="VAH76_002625"
     CDS             348..1286
                     /locus_tag="VAH76_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEO1387216.1"
                     /translation="MWFNNALMFQYKLDGGCDLNRSLTEEALKPCPPHARFTYGWVPA
                     FEQEMVHEVAGSTLICLGKEERILPRGVINKMLKEKIQQIEAAQGRPVKRAEKAQMAE
                     DLEFELLPKSFCVQKKLLAILDSVSQRLIINTSSNNQASQLTSFLRKSIPGIVIEPLN
                     LTENLALRFAEWIHSPATLPDDFQLASDCLLFSLEDEKKRVHCKGYELPAEEILTLLA
                     QGMGTAEISLIWKERIQFTLTQDFTFKKLKSLDYLIDDFNEIRQLEEEYQRQDAALTL
                     LSGELRELINGLMKIFVNHAEMKSAQIEKIAEDCTI"
     gene            complement(1434..2207)
                     /locus_tag="VAH76_002626"
     CDS             complement(1434..2207)
                     /locus_tag="VAH76_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEO1387217.1"
                     /translation="MKRIHVLFLIGLVVGISSCTKWGKDDEDNNPYKGMTAKQLYTAA
                     QTSLKKEEYATAAKQLEAMESMYPFSDYTESSQMQLIYAYYKDEDYPSAAATAERFIH
                     LYPRAKNVDYAYYMRGLANFQQTRGVFAKMLPMDESWRDPGTQTQALVDFATLIQKFP
                     DSKYKANALQRMIYLRNMFAQHELNVSLYYFKRKMYVAAIERASYLVKNYPQAPSAQK
                     ALVVMYEANKALGLNKAAEDAMAVYKATYHTSNMIRINS"
     gene            2373..2460
                     /locus_tag="VAH76_002627"
     tRNA            2373..2460
                     /locus_tag="VAH76_002627"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Ser,seq:tga)
     gene            complement(2591..4978)
                     /locus_tag="VAH76_002628"
     CDS             complement(2591..4978)
                     /locus_tag="VAH76_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1387218.1"
                     /translation="MLTDAQIQQAINNLNILIAHYNSPISSFSFDNERITRNWFFKSE
                     SIHATALSNYLKKIIDEVSSDKVDKTNSLYASLFITLQNFLHIQPNRVIEGLNFNLEN
                     SQYLKRKNLIIKRLEHFHIENQFRDDLKQHLLPEIQYDTCTGHYTSPDDDKAHQHGSE
                     AQRIFFSTQWERLKALCNWLLAKMGIHIFETARYKEHDYLDSEVYANDPAIEPGVSPQ
                     QSATHYWIGHASNLMTIPAHDSPLHVVTDPVEGDLAPFLYPRMTKEGSLIDGTGSKKL
                     PKIDVVVISHNHHDHVSQSTLKRLLKQQPKMVVPEGDESFFLGMGFTDVVGLKWWEQA
                     KITDFQGNELLRVTAVPARHWSGRSLTDAHCSAFNGYVLRANQLDGDIYFAGDTALMD
                     DMLSQPIFEKFNIVTSIQPGGPDERREDMESTHQSSADAILMHFKILAAQYQKMKEKN
                     ESLSLDDFLMENQHLKTLYNHTATFKLGNLRLKDTYYSYQRMVAAFREDAQWRADHLP
                     KHEQKVYDHIQLLISNMVFKNNQRLSNRQIADLILGKVVIPKIGQRQPLYFKEKSIKT
                     EAFQYRNLITNRRALLEFDMLLQKSIAEANEFFSIKRLILNLLRAYQNPWHAFFSRTY
                     LGVGNYEQDIRNCQSNSDLLAVLNHMERNLGKRNPHGHMQSLIYYAKWVIDFSNKYQH
                     ETLMKFKEYFACQQIRKLADQEIHHAGLIVGFGDRQAKQQRFKILSNELADLPEDVTE
                     YRKAIDNWLAARVNDGQSTQQLLSENRSSVFAKKVTRGENTVLHIKTTLDMAVSS"
     gene            complement(5070..>6753)
                     /gene="ravS"
                     /locus_tag="VAH76_002629"
     CDS             complement(5070..>6753)
                     /gene="ravS"
                     /locus_tag="VAH76_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavS"
                     /protein_id="HEO1387219.1"
                     /translation="ERTCYVDFDGLTVAQLKSDWLTVNPQGDLALSEGVYPSFSQGDN
                     TGAVIGEILQTKHFSLPLSEEDPKAFMEVTRNTTRICFSYKNSSGQPSGFSLVAAPNG
                     DSEGKHKGWIISVIQNTVGNYKDRKVSVLASPNYVRSEAQSTVDMDTPDAIERELKRV
                     INNDKVASLIKGIFKEDGTLNADYIRQLASRIKGNRNIDDRDRKSAQINILIELAEQL
                     DDEKLRHYINSIKADMAADIEFFKDGIYEQHLSELSNKISLSLSKMPSVNKKTTEIGR
                     ILNNISERYAQIKMIEDAQRKNPELAHIFKAMAQKIKSDFHDIKFDGTHQDNRNILLL
                     SQFEVASVEQKIRNYYQTNPQVKQYFIDKDKANHHLQVDLNRLSPPKNEGFFSRNKDS
                     FGFSIFMGLIVGVSAALFLTGVLAPIGVALIAVGAALITTGLVGSIDIYANEKEYAKE
                     TSHYSNEVKCLRSAHREHLVRLEKNLHEQINLSDFLTPDKPEFLSGIKVNKKKPLTPE
                     RVAHALWLQDKSARDTSVAKNRFSIWAAKQENLDFGIEEVESVIKQRYVMKS"
BASE COUNT         2037 a         1317 c         1199 g         2200 t
ORIGIN      
        1 aagcttgcaa taaaaatata cttcctttta agttcaatgt tgacacagca acatttatgg
       61 ttgaaatacc caatattatg gcttcgaaag aaaaaagatc actaagtttt tattggcaag
      121 aaaaactgtt tgctttcttg atgcgcaatt actcagcgaa tttaaatatt gaattctata
      181 aattacctta caatagaact atagcaatag gtacctattg tattttatga gttttggtca
      241 gggtctgttg acactttgct ctcttgcgtc gaaatggcaa cagcccctaa tcatcgcagt
      301 aaaatgctat ccgtgataac atcccaaatt tattttaagg actaattatg tggttcaata
      361 atgcactgat gtttcaatac aaactagacg gtggatgtga tttaaaccgt tctttgactg
      421 aagaagcgct taagccctgc cccccccatg ctcgctttac ttatggctgg gttcctgcgt
      481 ttgagcagga aatggtgcat gaagtagctg gtagcacgct tatttgcctg ggtaaagaag
      541 agcgaatcct tcctagagga gtcattaata aaatgctcaa ggaaaaaatt cagcaaatag
      601 aagcagccca gggtcgaccg gtaaaacgcg cagaaaaggc tcaaatggct gaggatcttg
      661 aatttgaact attgcctaaa tccttctgtg ttcaaaaaaa acttttagcg attcttgata
      721 gtgtaagtca gagattaatt attaatactt caagcaacaa tcaggcgtct cagttgactt
      781 cttttttgcg taaatcaatc cctggtattg tgatagaacc attaaacctt accgaaaatc
      841 ttgccttgcg attcgcagaa tggatccatt ctccagcaac tctacctgat gactttcaat
      901 tagcctccga ttgtttactc ttttctcttg aagatgaaaa aaaaagagtc cattgcaaag
      961 gctatgaatt acctgccgaa gaaatcctga ctctattggc tcaaggtatg gggactgcag
     1021 aaatctcatt aatttggaaa gagcggattc agttcacctt gacgcaggat tttactttta
     1081 aaaaactgaa aagccttgat tatctgatcg acgattttaa tgagatcaga caattggaag
     1141 aagagtatca aagacaagat gccgcgttga ccttattaag tggcgagtta agagaactta
     1201 ttaacggatt aatgaagata tttgttaatc atgcagaaat gaaatcggct caaattgaaa
     1261 aaattgcaga agattgcaca atataaagtc gtatcatttt aacccgtaac ctatcgaggg
     1321 aactcctttg ctaacagagc tccctctcat cttgattcaa gagaaaacga gtgcattaca
     1381 catcacttgc cgaacccagc tgacaaaata aactctataa atttttgtac tcatcaagaa
     1441 ttaattctga tcatattaga agtatgataa gtagccttgt aaacagccat agcatcctct
     1501 gctgccttgt taagccctaa tgctttattg gcttcgtaca ttaccactaa agccttttga
     1561 gcgctgggag cttgaggata gtttttcact agataactgg cccgttcaat ggcagccaca
     1621 tacattttac gtttgaagta gtaaagagag acattgagtt cgtgctgagc aaacatattt
     1681 cgtagataaa tcatacgttg caaagcattg gctttatatt tgctgtcagg aaatttctga
     1741 attaaagtag caaagtcaac caatgcttga gtttgtgttc cagggtccct ccaggattca
     1801 tccataggaa gcattttggc aaatacgcct cgtgtttgct ggaagttagc caaacctctc
     1861 atataatagg cataatcaac gttttttgcg cgtggataca aatgaataaa gcgctcagcc
     1921 gttgctgcag cagaaggata atcttcgtct ttataatagg cataaataag ctgcatttga
     1981 gaactttcag tatagtcact aaaaggatac atagactcca tggcttccaa ctgtttggca
     2041 gcagtagcat attcctcttt ttttaaagag gtttgtgccg cagtgtagag ctgcttagct
     2101 gtcatgcctt tatatgggtt attatcctca tcatcttttc cccatttagt acacgatgag
     2161 attccaacaa ctaacccaat aagaaataac acatgaattc gtttcataaa gcacacctat
     2221 caattatatc tgctgtattt ataagtacag caactggaaa atttgggaca ttatacctag
     2281 gaccagttaa tttgcgaaca acctattatt cttttattta tctataaaaa aacatttgca
     2341 aaatgatagg cgttcatata gtattctcga ccggagagat ggcagagcgg tcgattgcgg
     2401 cggtcttgaa aaccgttgaa gggcaacctt cccagggttc gaatccctgt ctctccgcca
     2461 gacaacatta ccaaggtata ttttcccgag acaaccaatt tcactttagc gttattctat
     2521 tcttcaagta caaaaagtaa gtgaatgaca tctaacccat taataaaaaa gactaaagtc
     2581 attgcatcta ttaacttgat actgccatat caagagttgt tttgatatgg agcacggtat
     2641 tttctccgcg ggttactttt tttgcaaaaa cagatgagcg attttcacta agcaattgtt
     2701 gggtagattg tccatcatta actcgtgcgg caagccaatt atcgatggct tttctatatt
     2761 ctgtaacatc ctcaggcaaa tcagccaatt catttgaaag tattttaaac ctctgttgct
     2821 tcgcttggcg atctccgaaa cccactataa gaccagcatg atgaatttct tgatcagcca
     2881 attttcttat ctgttggcaa gcaaaatatt ctttaaattt cattaaggtt tcatgttgat
     2941 atttattgga aaaatcaatt acccatttag cataataaat caaactctgc atgtggccat
     3001 gtggatttct tttgcccaga tttctttcca tatggtttaa tacagcgagt aaatcgctgt
     3061 tactctggca attgcggata tcttgctcgt aattacctac tccaagatac gttcgggaaa
     3121 aaaaggcatg ccagggattt tgataggctc gcaacagatt taaaataagt cgtttgatac
     3181 taaagaattc attcgcctca gcaatggatt tttgcagcaa catgtcaaac tcgagtaaag
     3241 cacgacgatt agtaatcaga ttacgatatt gaaatgcttc tgtctttatc gatttttctt
     3301 taaaataaag tggttgtctt tgaccaattt tgggaataac taccttgccc agaatcagat
     3361 cggcaatttg ccgattacta agccgttgat tatttttgaa aaccatattg cttatcaaaa
     3421 gttgaatatg atcgtaaacc ttttgctcat gctttggtaa atgatcagct ctccattgag
     3481 catcttccct aaatgcagca accattcttt gataactgta ataggtatct ttcagacgta
     3541 aatttcctaa tttgaaagta gcagtatggt tataaagagt tttaagatgt tgattttcca
     3601 ttaaaaaatc atcaagagat aaactctcat tcttctcttt cattttttga tattgtgcag
     3661 ccaatatttt aaaatgcatc agaattgcat ctgcagaaga ttgatgagta gactccatat
     3721 cttcacgccg ctcatctgga ccaccgggtt gaatagaggt aacgatattg aatttttcaa
     3781 aaatgggctg tgataacatg tcatccatta acgcggtatc cccagcaaaa taaatatctc
     3841 catctaattg atttgcacgc agcacatagc cattaaatgc tgagcaatgg gcatcggtca
     3901 gcgatcgacc cgaccaatgc ctggcaggca ctgcagttac ccttaataat tcattccctt
     3961 ggaaatctgt aatctttgct tgttcccacc atttcaaccc tacaacatcg gtaaatccca
     4021 tgcctaaaaa gaatgattcg tcaccttccg gaactaccat cttaggttgc tgttttaata
     4081 ggcgcttcaa tgtggactga cttacatgat catgatgatt gtgagaaatc actactacat
     4141 caattttggg taatttttta ctgccagttc catcaattaa actcccttcc tttgtcatac
     4201 gcgggtatag gaaaggagcc aaatcacctt caacaggatc agtgactaca tgcaatggcg
     4261 aatcatgagc aggtatggtc attagatttg atgcatggcc aatccagtaa tgagtggctg
     4321 attgttgtgg gcttacccct ggttcaatcg ctggatcatt ggcatagacc tcgctatcca
     4381 aataatcatg ttctttataa cgtgcagttt caaaaatatg aatacccatt ttagccaata
     4441 accaattgca taaggctttc aatctttccc attgagtaga gaagaaaatt cgctgggctt
     4501 ctgagccatg ttgatgcgct ttatcatcgt ctggcgaggt ataatgccca gtgcacgtat
     4561 catattgaat ctcaggaaga agatgctgtt tcagatcatc cctgaattga ttctcaatat
     4621 gaaaatgttc cagtctcttg ataatcaagt ttttgcgttt caggtactga ctattctcca
     4681 aattaaaatt taagccttca attacccggt tcggttgtat atgaaggaaa ttttgtaagg
     4741 taataaacaa actagcatat aaactattag tcttatctac tttgtcgctt gaaacttcat
     4801 cgataatttt ctttaaataa ttactcaggg cagtagcatg tattgattca gatttgaaaa
     4861 accagttcct tgtaattcgc tcattgtcaa aagaaaagct ggatattggc gagttatagt
     4921 gtgcaataag gatattaaga ttattaatag cttgttgtat ttgagcatca gttaacatat
     4981 tttcttaccg tattatccaa tcagctgatt cgtttgctat attttgccaa gagttataaa
     5041 tatctgcttc attgaattct caatattttt tatgatttca tgacatatct ttgtttgatt
     5101 acgctttcca cttcctctat accaaaatcc aggttttcct gcttggcagc ccatatcgaa
     5161 aatctatttt ttgctactga agtatctcga gcacttttat cctgtagcca taaagcatgg
     5221 gctactctct ccggagtcag cggttttttc ttattgactt tgatgcctga taaaaactca
     5281 ggtttatcag gagttaggaa atcagaaagg ttgatttgct catgtaaatt tttttctaaa
     5341 cgaaccaaat gctccctatg agcactcctt aagcatttta cttcattaga ataatgcgat
     5401 gtttctttag cgtactcttt ctcattggca taaatatcga ttgaccctac cagtcctgtt
     5461 gtaatcaagg ctgctccaac agcaatgaga gctactccga tgggggcaag aacgccagtc
     5521 aaaaataatg ccgcactgac tcctacgatt agacccataa aaatactaaa tccaaatgaa
     5581 tctttatttc tactgaaaaa accctcattt ttgggaggag ataaccggtt tagatcaact
     5641 tgtaaatgat gatttgcttt atctttatct ataaaatatt gttttacctg aggattggtt
     5701 tgataatagt ttctaatctt ctgttcaaca cttgcgacct caaattgact taagagcaaa
     5761 atatttcgat tatcctgatg tgttccatca aattttatat catggaaatc ggattttatt
     5821 ttttgagcca ttgctttaaa aatatgggct aactccgggt tctttctctg tgcatcctca
     5881 atcattttaa tctgtgcata acgctcagaa atattattta atattctgcc tatttcagta
     5941 gtttttttat ttacagaagg catcttactt aacgataagg aaatcttgtt ggataactca
     6001 cttagatgct gctcataaat tccatctttg aaaaactcaa tatctgcagc catatcagct
     6061 tttattgaat tgatatagtg tcttaatttt tcatcatcca attgttctgc caattcgatg
     6121 agaatattga tttgtgcaga ttttcggtca cgatcgtcta tatttcgatt ccctttgatc
     6181 cgtgaagcca attgtcgaat ataatctgca ttgagtgttc catcttcctt aaaaatgcct
     6241 tttattaaac tggcgacttt atcattatta atgactctct ttaattctct ttcaatagca
     6301 tcaggagtgt ccatatcgac tgttgattga gcttcagacc ttacataatt tggagaagca
     6361 agaactgata ctttcctgtc cttgtaattc ccaacggtat tttggataac tgaaataatc
     6421 cagcctttat gttttccttc actatcacca tttggagcag caaccaggga aaaccctgat
     6481 ggttgtccag aagaattttt ataagaaaaa caaattctcg ttgtatttct agttacttcc
     6541 atgaatgctt tagggtcttc ttctgacaaa ggtaacgaaa aatgtttggt ttgaagaatt
     6601 tctcctatta ccgcaccggt attgtctccc tgagaaaagg atggataaac accttctgat
     6661 agcgccaaat caccttgtgg attgacagtt aaccaatcag attttaattg cgcaacagta
     6721 aggccatcaa aatccacgta acaggttctt tca
//
LOCUS       DATNSN010000160         6683 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.160, whole genome shotgun sequence.
ACCESSION   DATNSN010000160 DATNSN010000000
VERSION     DATNSN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.160"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..296)
                     /locus_tag="VAH76_002630"
     CDS             complement(<1..296)
                     /locus_tag="VAH76_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387220.1"
                     /translation="MTSNKCDIVLLTQKDFIDPADITPYIQNVLTEDRLLTEALEQKG
                     LKVKRTHWDNRQYNWSETRYALFRATWDYFHRYDEFHLWLNDCSKRIQFINPIS"
     gene            477..1244
                     /locus_tag="VAH76_002631"
     CDS             477..1244
                     /locus_tag="VAH76_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HEO1387221.1"
                     /translation="MFKNAIVRTPSSTMINGLTTSADLGKPNYELALIQHQNYIKALS
                     TCGLEVTVLPDLEQFPDACFVEDVSLLTEQFAVLTCPGAESRRDEVQEIEPSIQAFFK
                     DRIFRISPPGRLEAGDVLRIDNHFFIGLSERTNKEGAEQLIYLLNQHGYTASVIQLKK
                     FLHLKTGVSYLNNDYVLVSGELINHQAFSHLKQIVVSPEEAYAANCIMINEIVLLPKG
                     CPKITHRLSELGFSIIELDMSEFRKLDGGLSCLSLRF"
     gene            1244..2560
                     /locus_tag="VAH76_002632"
     CDS             1244..2560
                     /locus_tag="VAH76_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO1387222.1"
                     /translation="MKSRLSVLSLALITVCSVDSIRNLPAAALAGDQLFYYFALALLF
                     FLLPCAIVAVWFSRQSEQGVYGWVKQGLGNQWAFIAIWFQCIQNVLIYPTLLSFIAGT
                     LLYSISPDLVQNKSMLFLIIIFLIWSLTWINLKGIHLSSRFNSYCSITGLILPFIIIL
                     FVGLYWWITQVKPGTTVLPPAEPYSWTSLTAIILSFCGIELAAVHARDSKQGAFPKAI
                     AISVIIIFLTMLFGSIVLAMIIPPEQLSFISSIPQLIQLFFNKIGYGYFAFIINGLIA
                     IGCIGAANNWLIAPIKGLEFAISEGFLNRQLSQVNKHNAPFRLLVAQAFCVSIISALF
                     LIVPSINASYWIMLNAATQINLLMYLLLFISAIKIVSSKPILSKLNIMIFAYLGLAGS
                     SITLIVSLTPPPSFAISSKLIYALLGAIFLVIMTLIPIWRKPKVLT"
     gene            2696..3733
                     /locus_tag="VAH76_002633"
     CDS             2696..3733
                     /locus_tag="VAH76_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEO1387223.1"
                     /translation="MKLTRNMIAQAVQAKIITEEQAKNLIEFIKNLPEQTPGFNLTNV
                     LYYFGGLIAIGAMTLFMNLGWELFGGWGIFYLSICYALLGLGLCSQFNGKGYVIPAGI
                     CATFVICLTPLAIYGLQKGMGWWPDDSVYHDYHVYIKWHWIFMELGTLMVGIILAWIY
                     RYPFMIMPIAVTLWYMSMDIAAMLGDGQFDFELRAMVSMYFGLITVLIAFWVDVRSSQ
                     SADYAFWLYIFGVMAFWFGLSCQSSDSEISKFIYMCINLFMILTGVILNRKVFVIFGA
                     LGCSYYLGHLAFQVFKFSMLFPVALTAIGFGIIYLGIIWQRNEAKLTKELRSILPKPL
                     RDLLEARDSMD"
     gene            3872..5353
                     /gene="legL3"
                     /locus_tag="VAH76_002634"
     CDS             3872..5353
                     /gene="legL3"
                     /locus_tag="VAH76_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL3"
                     /protein_id="HEO1387224.1"
                     /translation="MLTLSLAKCINATKNGAFAIKDLDLSGPLSYEDVLFLAEYLKVT
                     PVTDLDLSMTITRENSKALWDLSQVIAKNSLLQKLTFEAQLDFDFYFKHNLPIGWLDF
                     IDDYRLKKIAAPLHEALVNMLKNKPALNKLTIDFLAMETPFHNKHLDQLARSINKISL
                     NYLNLGLSVTKETQVPLFKTKEPNQTLEFLILRGFNSGRNTLKYLPQTAALKMLAIDD
                     MQFEEHDLKLLANIMKSNSSLVALILGKTNLGQVNSPDVLTWLSACPNLNSLILIDNN
                     LSRLNVDSLCELLANNLQALKKLDLSKNAYMGDDAIKIAKALTFNAHIETLVLDDNYI
                     ENEGLQAIINLVKTNKNITSLSISNTCRYTPSNEIIHSLCDLLRDPGCQLKELKFNQD
                     ITLAQYLMLQHALNSNKTLEYIDFNFRNQNILEAILGKNNSSDLNENEFSSDNPKEEK
                     APNTLNTDPTSKSAPLFFISPKSRHEENENELSSLSSHSVHLH"
     gene            complement(5447..6565)
                     /locus_tag="VAH76_002635"
     CDS             complement(5447..6565)
                     /locus_tag="VAH76_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1661 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387225.1"
                     /translation="MNTVPLKPQRLLSLDVFRGMTIVLMIIVNGQAAIDPYPIFEHVD
                     WNGCTLADLVFPFFLFIVGLTSVISLKNQMERKAKTSLYSAIIERSVVLFLLGLFLNV
                     FPHPIEFDSIRIYGILQRIAVCYLISAFIYLNTSIKTQFFIFLVLLLGYWIIMTQVPV
                     PGYGANQLTKDGSWVSYFDQLFFSASHLYEKTYDPEGFLSTFTSIATTLSGVLAGSLL
                     INPCNQFKKFYLLAGVGLLFLLLGWLWNMSFPINKNLWTSSYVLWTSGLALLAFAFCY
                     LLIDRLGVKKWSVFFKIFGMNALFAFVFHVLLLKLQYAFKITTPDGSKMALISYLKDY
                     FFGGFSNHNAALLYSICFLFLNFLVVLILYKRNVFIRI"
BASE COUNT         2150 a         1217 c         1196 g         2120 t
ORIGIN      
        1 atagaaatcg gatttataaa ttgaattctt tttgagcaat catttagcca gagatgaaac
       61 tcgtcataac gatgaaaata atcccaggtg gcacgaaaca gtgcatatct agtctctgac
      121 caattgtatt gtcgattgtc ccaatgagtt cgtttaactt ttaatccctt ttgctccaga
      181 gcttcggtta ataatcgatc ttctgttagc acattttgaa tgtagggagt aatatcggcc
      241 gggtctataa aatccttctg cgttagtagg acaatatcgc acttgttaga ggtcatgata
      301 gaaacaatcc gtatcaaatg atacaagaat acttatcttt ttaaactaaa tctatatacc
      361 ccatcgcaaa tcaaccagct aatttaatca aattaacaat gattgcattt tgaagactct
      421 ttgtttaatc tctggctgcg tgtgtattgg aaatcactta tttaaggaca tgatgaatgt
      481 ttaaaaatgc tatagtaaga acaccatctt ctacaatgat caatggacta accacatcgg
      541 ctgatttggg gaaacccaat tatgagcttg cgttaataca gcatcaaaat tatatcaaag
      601 cactatcaac atgtggtctt gaagtgacag tgcttccaga tctagaacaa ttcccggatg
      661 cttgttttgt agaagacgtg tcattattaa ctgaacaatt tgctgtcttg acatgccctg
      721 gcgcagagtc caggcgagat gaagttcagg aaattgaacc cagcattcaa gcattcttta
      781 aagacaggat tttcagaata agcccgcctg gcagacttga ggctggagat gtgcttagaa
      841 tagacaatca tttttttatt ggtctctcgg aaagaactaa caaagagggg gctgagcaac
      901 taatttactt actgaatcag catggctata cagcctcagt aattcagcta aaaaaatttc
      961 tgcatcttaa aacaggagta agttacctga ataatgatta cgtattggtg agtggagaat
     1021 taatcaatca tcaagcattt agtcatttaa aacaaatagt agtaagtcct gaagaagcat
     1081 acgcagctaa ttgtattatg attaatgaga ttgtgttatt acccaaaggg tgtcctaaaa
     1141 taactcatcg tttatctgaa ctggggtttt caattattga attggatatg agcgaattta
     1201 gaaagttaga tggtggttta agctgcttgt ctttgagatt ttaatgaaaa gccgcttatc
     1261 tgttttaagc cttgccttaa tcactgtttg ctcggttgat agtattagaa accttccagc
     1321 agctgcttta gcgggggacc agctgtttta ttactttgca ttggccttgc tattctttct
     1381 tctcccttgc gccattgttg cagtttggtt ctccagacaa tcagaacaag gtgtgtatgg
     1441 gtgggtaaaa cagggactag ggaatcagtg ggcattcatc gccatttggt ttcaatgtat
     1501 tcaaaatgtt ttaatttacc ctaccttatt gagttttatt gctggcaccc tgctctacag
     1561 catctcccct gatttagttc aaaacaaaag tatgcttttt ctaattatta tttttttgat
     1621 ttggagttta acctggatta atttaaaagg aattcatctc tcaagtcgtt ttaactctta
     1681 ttgctccata acgggattaa ttcttccttt tattattatc ctgttcgtgg gattgtactg
     1741 gtggattacc caggttaaac ctggtaccac agttctacca ccagccgagc cttattcctg
     1801 gacttcgctt acagcaatta ttttgtcatt ttgcggtatt gaactagccg cggttcacgc
     1861 acgggatagt aaacaagggg ctttccctaa agccatagct atttcagtta ttataatctt
     1921 tctgacgatg ttatttggtt cgatcgtatt ggctatgatc attcccccag aacaattaag
     1981 ttttatcagc agcattcccc agctcataca attatttttt aataaaattg gttatggata
     2041 ttttgctttt attatcaatg gattgattgc aataggttgt attggcgcag caaataactg
     2101 gctcattgca cctataaaag gattggaatt tgcaataagt gaagggttct taaataggca
     2161 acttagtcaa gtgaataaac ataacgcacc tttcagattg ttagtcgcac aagccttctg
     2221 tgtatcgatt attagtgctt tatttcttat cgtaccttcg attaatgcat cttactggat
     2281 catgttgaat gcagcgacac aaatcaattt attaatgtac ctcttgctct tcattagcgc
     2341 tatcaaaata gtttcctcaa aaccgatttt aagtaagcta aatataatga tttttgctta
     2401 cctggggttg gcaggcagta gcattacttt aattgtaagt ttgacaccac cgccatcatt
     2461 cgccattagc tctaaattaa tttatgcatt gcttggcgca attttcctgg tcataatgac
     2521 cttaattcct atttggagga aacctaaagt tcttacatga aaagaaatat ccaatcctga
     2581 tttgcaaatt caaacgcaag tttcatactc tcagctataa ttgagatatt aatcattagt
     2641 tattgtaaaa tcaggttaaa ttttagcagg aattcagtac tcatcggaga tgatgatgaa
     2701 attgacacgg aatatgattg ctcaggctgt ccaggctaaa ataattacag aggaacaggc
     2761 caagaacctc attgaattca taaaaaatct tcccgaacaa actccaggat ttaacttaac
     2821 caatgtcctt tattattttg gaggacttat agctattggt gcaatgactt tgtttatgaa
     2881 tcttggatgg gaactgtttg gaggctgggg tattttctat ttgtcaattt gttatgccct
     2941 actcggccta ggcctttgca gtcaattcaa tgggaaagga tatgtcattc ctgctggcat
     3001 ttgcgctact tttgtcattt gccttactcc tttggcaatc tatggcttac aaaaaggaat
     3061 gggctggtgg ccagatgaca gtgtttatca tgactatcat gtttatatca aatggcattg
     3121 gatcttcatg gagttgggta cgctaatggt aggtattatc ctggcctgga tatatcgata
     3181 cccttttatg attatgccaa ttgcggtcac tctttggtat atgtccatgg atatagcagc
     3241 catgttgggt gacggtcaat ttgattttga gctaagggca atggtttcaa tgtactttgg
     3301 cttaattact gtattgattg ccttttgggt tgatgttcga tccagtcaat cagcagatta
     3361 tgctttttgg ctgtatatat tcggtgtcat ggctttttgg ttcggtttaa gttgccaatc
     3421 ctccgacagt gaaatatcca aatttatcta tatgtgcatt aacctcttca tgattctgac
     3481 cggagtcatt ttaaacagga aagtattcgt tatttttggt gcgttaggat gttcttatta
     3541 tctggggcat ctggcattcc aggtgtttaa attcagtatg cttttccctg ttgctttgac
     3601 tgccataggt tttggtatca tttatttagg aataatctgg caaagaaatg aagcgaaact
     3661 tactaaagag ctccgatcca tacttcccaa accattaaga gacttattgg aagcaaggga
     3721 cagtatggat taatgaaata ttttctcccg tcctatcaca ttctattact cttttagata
     3781 aattgtaggt aactctttac tttcaagtac ctgaagtaga agatagatga gttcatcatt
     3841 attttctttc aaaaggactt gaggagtaat tatgttaact ttatcacttg ccaaatgtat
     3901 taatgcaaca aagaatgggg cattcgccat caaagatttg gatttatcag gccctctatc
     3961 ttatgaagat gtattatttt tagctgaata tctaaaagtc acacccgtaa cagatcttga
     4021 cttgtcaatg accatcacca gggaaaactc taaagcgcta tgggatttaa gtcaggttat
     4081 tgctaaaaat tcactgctac aaaaactaac atttgaggcg caactggact tcgatttcta
     4141 ttttaaacat aacctaccaa ttggttggtt agattttata gacgattatc ggttaaaaaa
     4201 aatagcggcg ccactccatg aggcattggt taacatgcta aaaaacaaac ctgctctaaa
     4261 taagttaact atagactttt tagcgatgga aactcctttt cacaataaac atctcgacca
     4321 gttagcacga tctatcaata aaatctcgct taattatttg aatttgggcc tttcagtgac
     4381 caaagaaaca caagtaccgc tatttaagac aaaagaaccg aatcaaactc ttgagttcct
     4441 aatccttcgt ggttttaatt caggtagaaa taccttgaaa tatttacccc aaacagcagc
     4501 cttaaaaatg ttagctattg atgacatgca attcgaagaa catgatttga agttgctggc
     4561 taatattatg aaatccaact cgtcactggt tgctttaatc cttggtaaaa ctaatcttgg
     4621 acaagtcaat tcgccagacg tactcacctg gctcagtgca tgccccaatt taaatagtct
     4681 tatactcatt gacaataatc taagcaggct taatgtggat agtttatgcg aattattagc
     4741 gaataatctc caggctttga aaaaactgga tctgtccaaa aatgcttata tgggtgacga
     4801 cgcgataaag attgccaaag cgttaacttt caatgctcat attgaaacat tggtactgga
     4861 tgataactat attgaaaatg agggactaca agccatcatc aatctggtaa aaaccaataa
     4921 aaatataacg tctctatcca tttcaaacac ctgtcgatat acaccttcta acgaaataat
     4981 tcacagtcta tgtgatttat tacgtgatcc tggatgtcaa ttaaaagaac taaaatttaa
     5041 tcaggatatt actttggcac agtaccttat gctgcaacat gctctgaact ctaacaaaac
     5101 actagaatac attgatttca attttagaaa tcagaatatt cttgaggcaa ttcttgggaa
     5161 aaataattcc agtgatttga atgaaaatga gttttcaagt gacaatccta aagaagaaaa
     5221 agccccaaat actttgaata cggatccgac tagcaaaagt gctccattat tttttatttc
     5281 cccaaagagt cggcacgaag agaatgaaaa tgaactttct tctttatcga gtcatagtgt
     5341 ccatctccat tagggacaac acacattaca ctctattata atttctcact ggcgatatac
     5401 acagttggcg ctgagcaatc tgcgaggccc aacctacctt acctctttat attcgaataa
     5461 atacattacg tttatataaa atcaaaacaa ccagaaaatt caggaaaaga aaacagatag
     5521 aatacagcaa tgctgcattg tgattactga atcctccaaa aaaataatct ttgagatagg
     5581 atatcaatgc cattttagag ccatcaggtg ttgttatttt aaaagcatat tgcagtttta
     5641 aaagaaggac atgaaataca aaagcaaata atgcattcat gccaaatatt ttaaaaaaaa
     5701 cggaccattt tttaacccca agtctatcaa tcagcaaata gcaaaaagca aaggcaagca
     5761 aagccaggcc gctggtccag agtacgtaag aactagtcca taaattttta ttaataggaa
     5821 aactcatatt ccataaccat cccaataaca aaaatagcaa gccaacccct gctaatagat
     5881 aaaatttctt gaattgattg catggattga ttagtaaact accggcgagg acacctgata
     5941 aagtagtggc aatagatgta aaagtactta aaaaaccttc gggatcatac gttttttcat
     6001 acaagtggga ggctgaaaaa aataactggt caaagtatga aacccaactt ccgtctttgg
     6061 ttaattgatt tgctccatat cctgggacag gtacctgagt cataataatc caatatccta
     6121 ataaaagaac aagaaaaata aaaaattgag ttttaattga agtattcaga taaataaaag
     6181 cggaaattaa ataacaaaca gcgattcgct gcagaatccc atatatcctt atgctatcaa
     6241 attcaatagg atggggaaaa acattcaaaa ataatcctaa aagaaacaaa acaacacttc
     6301 tctctatgat agcagaatat aatgaggttt ttgcttttcg ttccatttgg tttttcaggc
     6361 ttataacact cgttaagccg acgataaaca agaaaaatgg gaaaaccaaa tccgccaaag
     6421 tacaaccatt ccagtccaca tgctcaaaaa tcggataggg atcaatagct gcctgaccat
     6481 taacgataat catcaataca atggtcatcc cgcgaaatac atcaagggat aaaaggcgtt
     6541 gaggttttaa gggaacagta ttcatgcgct atttaaaatg aatttataaa aaactgagcc
     6601 ccattatctt accacgagtg gcaaattgta atatcaaaaa taaaaattaa atttctaaaa
     6661 tctatatgaa aatctctttt aaa
//
LOCUS       DATNSN010000161         6541 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.161, whole genome shotgun sequence.
ACCESSION   DATNSN010000161 DATNSN010000000
VERSION     DATNSN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.161"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(325..840)
                     /locus_tag="VAH76_002636"
     CDS             complement(325..840)
                     /locus_tag="VAH76_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1387226.1"
                     /translation="MFNVSIRKATSLDAEAIASVHVHSWKKAYQQFIPEIVLNSLSIP
                     ERTQLWNELLNNGVTVLVLEIDNQIIGFASICTFRDGIEDNSKGEISAIYIHPNFWHQ
                     GFGKKLSLAALKTLKELGYTEALLWVLSDNHQARSFYEGMGFELNDKSKLAEFYDGGA
                     LLKEVLYKKKL"
     gene            complement(1593..4088)
                     /locus_tag="VAH76_002637"
     CDS             complement(1593..4088)
                     /locus_tag="VAH76_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387227.1"
                     /translation="MKLPFKEPVAPRYIYINPQTNVVHLLMPVMSGTEIGLDNTCKSV
                     YSLLEFFCLLGANQQSAASMILKNYQEGLAFDIKYHPNSEQKALKEQRLVQINMYLRL
                     LQQVQQEEQITNPLKLIFPTYPAALESLMQASEANLHSVILRPEEQDVQLRTTAISPV
                     FSAHHDTMVNGQRIEKKSSLYEILSNSYEGLVFIPKSKEQLIERVLSKCADSPVDFEH
                     IRAMLTQETKAYLGIEESFNQTQGNRYAPSAPVNQAYIDGELMISAEYPATPQDYLNA
                     LLEYCTPNLFDNIEESPFYTINNKERLSILTQFFLAELNIICREEGIAETNFGQILEA
                     NPELINNLAKCVKQALTGYQSVEDALINYVNQHRDDFQFTSPIPQYSFPKLKERFNSH
                     YKQIKDSPHFDEFMLLSTKKGLFFAHQGCIATHFAHFMNALFFNDILDENTKTFLQSV
                     QQDFETIDKFENTIPHQNTHINAGMKEAVLDLSSMDNEALQDLYEDINSYQDSNLKEA
                     LLAQLKQERPDFKLQMNAKIFLQHVAYGEQDEAEELLNKDPELTQELLRANNIFFTDY
                     SGRTFTCTAYEYAYWAKDSHMQRMLEKYIRQDDETRQFMLERVKAIEELVNPPEAERF
                     FAHPKPRGLHYTTQNKEGKTIDHWEAHVDLTPLKKALQHFLDEFKKLYNNPKPNNYRE
                     VLNNIWVKEVGMAQRCVPAHIAQEYCRVGWDLFLRLKDNKALFDASNPNNLKRQLKIF
                     DWNTYTYDLWFTPGSHAVDCGLGFSCALIRGGNNCPRPTNMYDRWTTPNEVVEQDLEM
                     ISIIDEVRTCDLKQSLENLSQPLIAQAAQYLSI"
     gene            complement(4388..5473)
                     /locus_tag="VAH76_002638"
     CDS             complement(4388..5473)
                     /locus_tag="VAH76_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO1387228.1"
                     /translation="MKSPLFLLILITTTLNAADIDYIIDTDIGGDIDDVLALFVALDN
                     PVKPLAITTTHIEPREKAQIAKLVVTYAGFSEIPVYAGIGTTRNDPRELFLQQNPLWP
                     PFFGYPDSLPGESPWFPQQAVPYRQMNHSIFDEMNIEEESAPEYLARIAKNYSPQHKL
                     VIVSLGPLHNISAALKINPSISSNLKIYSMGGIYPKGYNWLVSPEVTAPVLGSTEVIS
                     IVSQFVDAHDFYITPEEFLEIEKSAQSEFGLAVIQDWKNWYNIDKRNLNKTHLSDPVT
                     LYLALHPEEITHMSSKLLTFPCLDLNGVLAPEFAGSWYNMPGLENKLMKVEDSEESHI
                     KFVSQVESSSAIRRKIINSIKQILGNS"
     gene            complement(5918..6388)
                     /locus_tag="VAH76_002639"
     CDS             complement(5918..6388)
                     /locus_tag="VAH76_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEO1387229.1"
                     /translation="MVNKFHSILLFISLLFSCTAYANIISGLVIKISDGDTLTILTKD
                     NVQLKIRLSEIDAPEKKQPFGNKSKQSLSNLCFNKVAIVDILKIDRYGRSVGRVKCDN
                     VDANEYQVKNGLAWVYDKYVTDHSLYALQEQAKSKGIGLWSEKSPIPPWIFRHN"
     gene            complement(6403..>6541)
                     /locus_tag="VAH76_002640"
     CDS             complement(6403..>6541)
                     /locus_tag="VAH76_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387230.1"
                     /translation="LGFVTALGFAANTPCSGKKGGISHCLGEYFVCKDGSISQSKKQM
                     H"
BASE COUNT         1991 a         1214 c         1198 g         2138 t
ORIGIN      
        1 tagcatcaaa agtagagatt taaatagata tacaattaaa caagttgcaa gggtatattg
       61 aatgtcaatt tttaaaaaat tcaaaaaatt ttacagatcc tcatcagaga acaggacaca
      121 gatacacctg tttcttggct ttgttataat tccaataata ggcatgtctg ttttatatat
      181 ttgcgtaaga atattctgga tgtagcaaaa aactttgatt tttgaccaga gttttacaac
      241 cagttaagat ccaaatagaa aagctatatt cttttcctta cacagtccac aaacccatca
      301 ttttaagaat atgatatgtt gaggttataa tttcttttta tacagcacct cctttaataa
      361 ggctccacca tcgtaaaact ccgcaagttt tgatttatca tttaattcaa agcccatgcc
      421 ttcataaaag ctacgcgctt gatgattatc tgataaaacc caaagcaaag cttcggtata
      481 gcctagttct tttagggttt ttaacgcagc gagagacaat ttcttaccaa atccttgatg
      541 ccaaaaattg gggtgtatat aaatagcact aatttcccct ttagaattat cctcaatgcc
      601 atcgcgaaat gtacaaatac tcgcaaaccc aattatttga ttgtcaattt ccaatacaag
      661 tactgttacg ccattattta gtagttcatt ccatagttgg gtgcgctcag gaattgataa
      721 actattgagt actatttctg gaataaattg ttgataggct tttttccaag aatgaacatg
      781 tactgatgca atcgcctccg catccaaaga ggtagccttt ctaatagaaa cattaaacat
      841 gaattgatgc cccctttatt tagctttggg tctcgaataa tttaatttgt tttaccattt
      901 ttatgataaa agggacagta taccaaccaa ttaatacctc tgtaacataa ttcaatctta
      961 aggcaatata gaaaaaacca cctattaaga gtcagggtgt tatgtgcttg ataaaatccc
     1021 ccctgtattt gtgatagtgg acgaaattaa aaaagctgat tcaaccctgc aagaatactt
     1081 gattaaatca ctacgagaaa tcatgatttc tccatcattt aaaggttcta ttcctgggca
     1141 ttttgcccaa ttacggagca tttccaatga actttttcag aaatattgta catgagcatt
     1201 tcatatggaa ttagctatat gggtgaatta ttggatgaga cgggctcagt tgacaaagaa
     1261 ctgaaaaacc tcattgcggg tttttctgca tctagtacag gactagaata aaatctaaca
     1321 cgtgacgatt tatttttggg tgacaaaatc gttgacccgc catgcatctt aatgccttca
     1381 tccgcttctt gagatccatt aagacgagcc ggaccatctt gtatttggct tggaatagat
     1441 gcttccttgc ttatgaacca cctcattggg aaataattat aaagcgctga ttccaagctt
     1501 gccgtctttt atggataaag gcaaagagca agatctgtag gtgctcattt atgacagtca
     1561 catagcagga atcaccatgg ggctgttact aactaaatgc tgagatactg agctgcctgg
     1621 gcgattagcg gctggcttag attttctagt gactgcttga ggtcacaagt cctcacctca
     1681 tcaataatcg atatcatttc caaatcctgc tcgacaactt cattaggtgt agtccatcgg
     1741 tcgtacatgt tcgtggggcg ggggcaatta ttccctcccc tgattaatgc acatgagaat
     1801 cccaatccgc aatctaccgc atgagaacca ggagtaaacc acaaatcata ggtgtaagta
     1861 ttccagtcaa aaatcttaag ctgccgtttt agattattcg gattagaggc atcaaacaaa
     1921 gccttattgt cctttaacct taaaaacaaa tcccacccta cgcgacagta ttcttgggca
     1981 atatgagcag gaacgcatcg ttgagccata ccaacttctt ttacccatat gttattaagc
     2041 acctcgcgat aattattagg cttaggatta ttataaagct ttttaaactc atccaaaaaa
     2101 tgctgtaatg ctttttttaa tggtgtgagg tcaacatgag cttcccaatg gtctattgtc
     2161 tttccttcct tattttgggt ggtataatgt aagccccgcg gttttgggtg cgcgaaaaat
     2221 ctttcagcct ctggaggatt taccagttct tctatagcct ttacccgctc aagcataaat
     2281 tgtctggttt catcatcttg tctgatatat ttctctaaca ttctctgcat atgagaatct
     2341 ttagcccaat aggcgtattc ataagcggtg caagtaaagg tgcggcctga gtaatcggtg
     2401 aagaaaatgt tattggccct caagagctcc tgggtgagtt ccgggtcttt gtttaggagt
     2461 tcctctgcct cgtcttgctc accataagca acatgctgta aaaatatttt ggcgttcatt
     2521 tgcagtttaa aatcagggcg ttcttgtttt agttgggcta aaagcgcttc tttgaggttt
     2581 gaatcctggt agctgttaat atcttcatac aaatcctgca aggcctcatt atccattgag
     2641 gataaatcaa ggaccgcttc cttcatgcct gcattaatat gcgtattttg atggggtatt
     2701 gtattttcaa atttatcaat tgtctcaaag tcttgttgta cgctttgcaa gaaagttttt
     2761 gtgttctcat ctaatatatc attaaaaaat aaagcgttca tgaagtgggc aaaatgggtg
     2821 gctatgcatc cttgatgagc gaaaaataat cctttcttcg tgcttaataa cataaattca
     2881 tcaaaatgtg gtgagtcttt gatttgtttg taatgagaat tgaagcgttc ttttagtttt
     2941 ggaaagctat attgtgggat agggctagta aattgaaaat cgtctctatg ctgattgaca
     3001 taattaataa gtgcatcttc tacagattga tagccagtca acgcttgttt tacacatttg
     3061 gccaagttat taatgagttc aggatttgct tctaaaatct ggccaaaatt tgtctctgct
     3121 atgccctctt cacgacatat gatgttaagc tctgctaaaa aaaactgagt tagaatagat
     3181 agtctttctt tgttatttat cgtgtaaaaa ggggattctt cgatattatc aaacagatta
     3241 ggagtacaat attcaagtaa agcattgaga tagtcttggg gcgtggcagg atattctgca
     3301 ctaatcatca gttccccatc aatgtaggct tgatttactg gagcagaagg agcataacga
     3361 ttaccttggg tttgattgaa gctttcctca atgccaagat aggctttggt ttcttgagtt
     3421 aacattgcgc gaatatgttc aaaatcgacg ggtgagtccg cgcacttcga taaaacccgt
     3481 tcaatcaatt gctccttgga cttggggata aaaactaacc cttcatagct gtttgataat
     3541 atctcataaa gcgatgactt tttttcaatc ctttgaccat tgaccatagt atcatggtgg
     3601 gcactgaata ctggggatat tgctgtcgta cgtaactgaa catcctgctc ttcaggccgc
     3661 aatatcacac tgtgcaagtt tgcttcgcta gcttgcatta agctttctaa tgcagcagga
     3721 taggtgggga atataagctt taaggggttt gtaatctgtt cttcttgttg aacttgttgc
     3781 aacagtctta aatacatatt gatttgcaca agacgttgct ctttaagtgc tttttgctcc
     3841 gaattagggt ggtattttat atcaaatgcc aatccctctt gataattctt aagaatcata
     3901 gatgctgcac tttgttgatt ggcgcctaaa agacaaaaaa actccagtag ggaatagacc
     3961 gacttgcatg tgttatcaag gccaatttct gtaccactca tgacaggcat taataagtga
     4021 accacattcg tttgaggatt gatgtaaata tatcttggag ctaccggttc cttgaagggt
     4081 aatttcattg tattaacctg aatttgctat gtaaaatcaa tatattatca tcactacact
     4141 ggagtagcat aagttaatac aacatcttaa ggcaaaattg gtattcaact tttaacaaac
     4201 tgcgtatgta ttaaggaggg aataagggtc agatcctgaa tgcttatata ttaaagaaga
     4261 gagcactggg aaactcaagc agccagctca gactcttcgc ggacgctctt tgaatctgtt
     4321 tagaaaagcc aagctgagct gaaaagcctg agggatcctc gggcttgcca gctcagacta
     4381 cccttgctta agaattacct aaaatttgtt ttattgaatt aattatcttt cttctaattg
     4441 ctgatgagga ttcgacttga gatacaaatt taatatggct ttcttcgcta tcctctacct
     4501 tcataagctt attttcaaga ccaggcatgt tataccaaga acctgcaaat tcaggagcta
     4561 aaactccatt aagatcaagg catggaaaag taagtaattt agacgacatg tgggtaattt
     4621 cttctgggtg caaagccaaa tacaaggtaa caggatctga taaatgcgtt ttgttaagat
     4681 tcctcttgtc gatattatac caattcttcc aatcctgaat tactgccaat ccaaactcgg
     4741 actgtgcact tttttcaatt tccaaaaatt cttcaggggt aatataaaaa tcatgagcat
     4801 cgacaaattg actgactata gaaatcactt cagtagagcc aagcaccgga gctgtaacct
     4861 caggggaaac caaccaatta tatcctttgg gataaatacc gcccatagaa tatattttca
     4921 gattagaact aattgaagga ttaatcttta acgccgcaga gatgttatgt aatgggccta
     4981 aagagacaat tactaattta tgttgtggtg aataattttt agcaatacga gccaaatatt
     5041 ctggggctga ctcttcctct atattcattt cgtcaaaaat tgaatgattc atctgcctgt
     5101 aaggcactgc ttgctgagga aaccatgggc tttctcctgg taatgaatca ggataaccaa
     5161 aaaaaggagg ccatagcgga ttctgttgta aaaatagctc tctgggatca ttacgtgtag
     5221 tgccaattcc agcataaaca ggtatttctg aaaatccggc ataagtcacc acaagttttg
     5281 caatttgtgc cttttctctc ggctcaatat gcgttgtggt tattgccaag ggcttaactg
     5341 gattatcaag tgctacaaat aatgcaagta catcatcaat atcaccacca atatctgtat
     5401 cgataatata atcaatatct gcagcattta gggttgtagt gatcagaatt aataagaaaa
     5461 gcggggattt cagcctgcgc ttgctatgct aaagtacact taaagttcag agatatgttt
     5521 atagatattc aaaccattaa aaggaatttt agtttttata ccaacgttat actgattttt
     5581 ttctcttata ttaggcttgg caattatgta tggttattgg tcaacgttcg gtattaatat
     5641 atttccattt ctaggattat ttgatctcat aagattttct gcatatccat tattgatact
     5701 ttaagttcaa ctattgttac tttactggct gtttcttgtt gcaaaatttg caacaactga
     5761 atctttatct aattcattgg tgttaaatat attacttaag tgtcgtgaaa taaccgacct
     5821 gtcagaatgg aaaataatat taatgaacgt attcctgacg ctatttttat ttattttcca
     5881 ataagagttg taatggggtc acttccgaaa atataggcta attatgtctg aatatccaag
     5941 gagggatggg ggacttttcc gaccacaaac ctattccctt gcttttagct tgttcctgaa
     6001 gcgcatataa agaatgatct gtaacatatt tatcatacac ccatgcaagc ccatttttta
     6061 cttggtattc attagcatca acattatcac atttaactct gccaacagag cggccatatc
     6121 tatcaatttt taaaatatca actatcgcta ctttattaaa acaaagattt gaaagcgact
     6181 gcttggattt atttccaaat ggttgttttt tctctggtgc atcaatttct gataaacgaa
     6241 ttttaagttg aacgttatcc ttagtgagaa tagttaatgt atcaccatct gatattttta
     6301 taaccaaccc actaatgata tttgcatagg cagtgcaact aaaaagaagg gaaataaaaa
     6361 gcaaaattga gtgaaattta ttgaccattt tttttatcta aatcaatgca tttgtttttt
     6421 tgattggctt attgagccat ctttacatac gaaatattca cctaaacaat gagaaatacc
     6481 tccctttttt ccagaacaag gtgtattcgc agcaaaacca agagcagtta caaatcccag
     6541 g
//
LOCUS       DATNSN010000162         6482 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.162, whole genome shotgun sequence.
ACCESSION   DATNSN010000162 DATNSN010000000
VERSION     DATNSN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.162"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..481)
                     /locus_tag="VAH76_002641"
     CDS             complement(<1..481)
                     /locus_tag="VAH76_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387231.1"
                     /translation="MKIVRIFLSFLLLFFNGLGFARSELPQLFLFLGGDKASSFQETL
                     QNPCIRGVQIIYSWRQLEPQKGVYDFSKIEEDLNDLSRIDKKLFIQIQDRSFSPDVFP
                     VPDYIRLEKAYHGGVVQQYDFPGEGKPVTAGWVARTWDPAVRKRFQLLLKKLASKFDG
                     "
     gene            complement(770..1087)
                     /locus_tag="VAH76_002642"
     CDS             complement(770..1087)
                     /locus_tag="VAH76_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEO1387232.1"
                     /translation="MTMMITTGNSFEGKVIKQYLGIVRGIVVRSPTISQGLMGGLKSI
                     VGGKIGAYSQMCEHAREEAFQLMIEHAQALNANGIIAMRYDTGEIGQAGTEVLCYGTA
                     VII"
     gene            1556..2605
                     /locus_tag="VAH76_002643"
     CDS             1556..2605
                     /locus_tag="VAH76_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387233.1"
                     /translation="MEHCMPKTKYALPPVVLYESHADRATSDFLIKQLPDLKKAGYTT
                     ICVDGMEPGASLEENISMMKILIKMQIKKLSELPLEHPEYEQGIAKLRSVVAKLDLFE
                     AMKEQGFKLGGIDLPVSEQLKEKSLNSIRREQTLTDNTLRHVKENDGGVVVVLGFGHC
                     IFQQMIKEQDENADQYLWYHVHNPDNETQAYKELVESYTKKGLSTYFPLGVNIFKSSD
                     KKLDTDFWNKVSANCYNYDPKALETSTASILKSLLGPEVTAHLRTDGQHHVDALISLE
                     TVQKKHQVKSSDFLRSLSKTLGGIHFEVAKIKTKDQVIIRGINEPEVAEQISKLSKKC
                     NPTFLKNIAYFKLSC"
     gene            2690..4939
                     /gene="katG"
                     /locus_tag="VAH76_002644"
     CDS             2690..4939
                     /gene="katG"
                     /locus_tag="VAH76_002644"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEO1387234.1"
                     /translation="MFKRTIPLFAAFTLAISPSVFPNYAHAQEDKPKTNQYWWPKMLD
                     LSPLRQPNATSNPMGEKFNYAEEFNSLDLNAVIEDLKKLMTTSQDWWPADYGNYGPLF
                     IRMSWHAAGTYRIYDGRGGANGGFQRFAPQNSWPDNANLDKARRLLWPIKQKYGRKIS
                     WADLLVLAGNVAMESMGFKTIGFAGGREDAWEAININWGPEGKWLESKRQDKDGKLEK
                     PLAATVMGLIYVNPEGPNGVPDPLAAAEKIRETFGRMAMNDEETVALIAGGHAFGKTH
                     GAASGKYLGPAPEAAGIEEQGFGWKNSYGSGKGKDTITSGLEGAWTVTPTHWSHNYLQ
                     NLFNFNWVKTKSPGGAIQWVPENSNASSMVPDAFDPSKRHAPVMLTTDLALKFDPVYS
                     KIAKRFLDNPKEFDDAFARAWFKLIHRDMGPRSRYLGSLVPKEVMIWQDPVPPVDYKL
                     VDANDIANLKGKILNSGLTTSELVKTAWASASTFRGTDMRGGANGARIRLAPQKDWPA
                     NDPQELAKVLKTLESIQNNFNNAQADGKKISLADLIVLGGNAAIEQAAKQAGYDIIVP
                     FTPGRTDATQEMTDVKSFEVLEPKADGFRNYFDKSNNMSPPEMLVEKANLLKLSVPEM
                     TVLVGGMRVLNANTGQNQYGVFTDKPGILNNDFFVNLLSMSTEWKKSSETEGIYEGYD
                     RKTGKLKWKATSVDLIFGANSELRAVAEAYATDDAKDKFIQDFVNAWVKVMTADRFDI
                     KAANANINS"
     gene            5422..5991
                     /locus_tag="VAH76_002645"
     CDS             5422..5991
                     /locus_tag="VAH76_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEO1387235.1"
                     /translation="MKNKLLPNLIIIALAATISANSITAQAKQNDQIQNSNEQFTQDP
                     FDTDPFFQSHDDVLLQIHKMQQAMDQFIKSQFSQMQNTLANDPHHNPFGSAKNIQITE
                     NKDELIYKIKLPQGSDSKVDITVKNGQLVLSANVTQKITREQDNNKSVSFSQSNYMQS
                     FPLPQSYDPKSMVTKMKDSNLIVTFKKHS"
BASE COUNT         2081 a         1322 c         1271 g         1808 t
ORIGIN      
        1 ttccatcaaa ttttgatgcc agttttttta ataataactg aaagcgtttt cttacagcag
       61 ggtcccaggt acgagctacc catcccgcgg taactggttt tccttctcca gggaaatcat
      121 actgctgtac cacacccccg tgatatgctt tttccaagcg aatgtagtca ggtacaggaa
      181 atacatctgg gctaaatgaa cggtcttgta tctgaataaa cagcttttta tcgattctac
      241 tgaggtcatt caaatcttcc tcaattttcg aaaaatcgta aactcctttt tgcggctcca
      301 attgcctcca ggaataaatg atttgtaccc cacggatgca aggattttgc agagtctcct
      361 gaaaagagct tgccttatct ccccctaaaa acagaaacaa ttgtggcaac tcagatcgcg
      421 cgaaaccaag gccattaaaa aataacaaaa gaaatgatag aaatattctt acaattttca
      481 tttggcttct tgttcatcta tcatgtaagg aataatatca tatcgtctct atggactctc
      541 accttagtga tgaattactt caaaaattaa ttccagctta tccattccca gtataattta
      601 acactattac aggaagccaa agggacaggc ctaatagaat tggcacagac aaaataccag
      661 tgagttccac caatcttgac gaagtgagat cagggcttga attttgtaat caaccatgca
      721 cttacattat gggaaatact ctatggtctg cacttgaaaa aacagcgcat caaataatca
      781 cagccgttcc gtaacataaa acttctgtgc cggcctgacc aatttcgccc gtatcgtaac
      841 gcatggcaat aatgccattg gcattgagtg cctgggcatg ttctatcatg agctgaaatg
      901 cttcttctct ggcatgctca cacatttgcg agtacgcacc aatttttcct ccgacgatcg
      961 atttcaagcc tcccatcaat ccctggctga tggtaggaga gcgcactact atacctctca
     1021 caatacccaa atattgttta attactttcc cttcaaacga atttcctgtt gtaatcatca
     1081 ttgtcataat ctgctcacta aatatccctt ttatctttcc atattagttt aaaatcttga
     1141 aaatagaagt ttattgaaaa aaattcttaa aactatacga gggaattcta aataaagact
     1201 tccttgttta taaagaaaac tcatttgccg gattacagat tagcattggc aagaatagtt
     1261 aatccatttt gtttatactg agaaatattc cctagtacca gattaggttt tttccatcta
     1321 atgaacagga taaacccaaa cgatagctac aacacatctt ggcaagtaaa atagccagaa
     1381 tcaaaatttc agcaatttct tcagaaaaca aataagaaat tcactttctt actatcttaa
     1441 ttctccaaat cacaattcaa tcagaaaagt tattctccag acaaccacac cccaacatgt
     1501 tgtccattat atgagcattg aaatttaatc tactatactt aaagatatac ttcacatgga
     1561 gcattgtatg cctaaaacca aatatgctct tcctccagtt gtgttatacg aaagtcatgc
     1621 ggatagagct acgagcgatt ttcttatcaa acaactacct gatttgaaga aagctgggta
     1681 tacaaccatt tgcgttgatg gaatggaacc aggcgcatcc ctggaggaaa atatttctat
     1741 gatgaaaata ctcatcaaga tgcaaatcaa gaaattatct gagctacctc ttgaacatcc
     1801 tgaatatgag caaggaattg caaaactgag aagcgttgtt gccaaattgg acctttttga
     1861 ggcaatgaag gaacaaggat tcaaacttgg tggaattgac ttaccggttt cagaacagct
     1921 taaagagaag agccttaatt ccataagaag agaacaaacg cttaccgata ataccttaag
     1981 gcatgtgaaa gaaaatgatg gaggagtagt cgttgtcctg ggatttggcc attgcatatt
     2041 ccagcaaatg attaaagaac aggatgaaaa tgccgatcaa tacctctggt accatgtcca
     2101 taacccggat aatgaaacac aggcctacaa agaattggtc gaatcctata caaaaaaagg
     2161 actctctact tatttcccac tgggagttaa tatttttaaa agctcagata aaaaattgga
     2221 caccgatttt tggaataagg taagcgccaa ttgctataat tatgatccaa aagcgcttga
     2281 aacatcgact gcttccattt tgaaatcctt actgggcccc gaagtgacag cgcacttgcg
     2341 cacagatgga caacatcacg tggatgcact gatatctctt gaaacagttc aaaaaaaaca
     2401 tcaggtaaaa tcttcggatt tcttgagaag tttaagtaaa accttaggtg gcattcactt
     2461 tgaagtagca aaaatcaaaa ccaaagatca ggtgattatt cgtggcatta atgaacctga
     2521 ggtagcagag cagatcagta agttatcaaa aaaatgtaac cctacttttt tgaaaaatat
     2581 agcgtacttt aaattatctt gttgaataaa cagttaaatg tgttattgct gcttaaaagt
     2641 aacatggagt atactttgcc ttttctgtat agaaaaggga gtcatttcta tgttcaaacg
     2701 aacaatacct ctgtttgcag cattcactct ggccatctcg ccttccgttt tcccaaatta
     2761 tgcccatgcg caagaagaca agccgaaaac caatcaatac tggtggccta aaatgctcga
     2821 tcttagcccg ctgcggcaac ccaatgccac ttcgaatccc atgggtgaaa aattcaacta
     2881 tgccgaagaa ttcaatagct tagatttgaa tgccgtgata gaagatctca aaaaattaat
     2941 gactacctcg caagactggt ggcctgctga ttatggtaac tatggtccat tattcatacg
     3001 gatgtcatgg catgcggcag gaacttaccg catctatgat ggccgtggtg gagcaaacgg
     3061 tggcttccag cgtttcgccc cgcaaaacag ctggccagat aatgccaatc tggacaaagc
     3121 cagacgttta ctgtggccaa ttaagcagaa atatgggcgc aagatttcat gggctgattt
     3181 gttggtactt gctggaaatg ttgcgatgga atctatgggc ttcaagacca ttggatttgc
     3241 tggagggcgt gaagacgcat gggaagccat caatatcaat tggggccctg aaggcaaatg
     3301 gctggaaagt aagcgccagg ataaagatgg aaaactcgaa aaaccgcttg ctgcgaccgt
     3361 aatgggctta atctatgtga atccggaagg accaaacggc gttccagatc ctcttgctgc
     3421 ggccgaaaaa attcgcgaga ctttcgggcg tatggccatg aatgacgaag aaacagttgc
     3481 tttaattgct ggcgggcacg cattcggaaa aacacatgga gccgcgtccg gtaaatatct
     3541 ggggccagcg ccagaagcag cgggcataga agaacaaggc tttggttgga aaaacagtta
     3601 cggcagcggc aaagggaaag atacgattac cagtggttta gaaggtgcct ggaccgtaac
     3661 acctacccac tggagccata actacttaca aaacctgttc aatttcaatt gggttaaaac
     3721 caagagcccc ggtggagcaa tccagtgggt gcctgagaat tcgaatgcct cgtcaatggt
     3781 tcccgatgca tttgacccca gtaaaaggca tgcgccggtc atgttgacca cagacttggc
     3841 actaaaattt gatcctgttt acagtaaaat tgccaagcgt tttcttgata atcctaaaga
     3901 gttcgatgac gcatttgctc gtgcctggtt taagctgatt caccgagaca tggggccacg
     3961 ttcacgttac ctcggctctt tagtgcctaa agaagtcatg atttggcaag atcccgttcc
     4021 accggttgat tacaaattag ttgatgccaa tgatattgct aacctcaagg gtaaaattct
     4081 caactcggga ttgaccacat ccgagctggt caaaactgct tgggcctcgg catccacctt
     4141 ccgtggcact gatatgcgtg gaggggcaaa tggtgctcga attcgcctgg cgccccagaa
     4201 agactggccg gccaatgatc ctcaggagct ggcaaaagta ttaaaaacat tggagagcat
     4261 tcagaataac ttcaataatg ctcaagccga tgggaaaaag atatctcttg cggatttgat
     4321 agttttaggc ggtaatgcag caatcgagca agcggccaag caagctggct acgacatcat
     4381 tgttcccttc acgccaggac gcacagatgc cacacaagaa atgactgatg tgaagtcttt
     4441 tgaggttctt gaacctaaag cagacgggtt tagaaattac tttgataaaa gcaacaatat
     4501 gtcgccacca gaaatgttgg tggaaaaagc gaatctgttg aaattaagtg ttccggaaat
     4561 gacggtttta gttggtggga tgcgtgtact taacgcgaat actggacaaa atcaatatgg
     4621 tgtattcact gacaaacctg gaattctaaa taatgacttt ttcgtcaatc tgctaagcat
     4681 gtcaacagag tggaagaaat caagtgagac agaggggatt tatgaaggtt atgatcgcaa
     4741 aactgggaag ctgaaatgga aagcgacctc ggttgatctc atcttcggcg caaattctga
     4801 gttaagagca gttgcagaag cctatgcaac cgatgacgca aaagataaat ttatacaaga
     4861 ttttgttaat gcctgggtta aggtaatgac agccgatcgc ttcgacatca aagcggcaaa
     4921 tgccaacatt aacagctaat cctcattctt tcagatttag ggtgacaaac tgtgactcga
     4981 tcatcgtttg ttaccctaag tcccaaatca ccggataagg ctatgactta tactggttta
     5041 acaaagcctg gctaatttag ccaaacctct atggttttcc agattggcct atgccctctc
     5101 ttgaattacg tgttacatgg ctgcattcca tttcaaatat tcgccacttt gtacttgaaa
     5161 aaatctggtt taacccaata tcttgcatgc ggctttcatg ttccccaatg agagataacc
     5221 gctttaaaat agacctcttg cctaagctga ttaattggtt tcaggccaag gcgctaacgt
     5281 tttgaggagc ggagtttcca ttcagcaata aaatgaaaga ggcaggttat gcaagagggc
     5341 taatgagtgg attgaagtaa aaacatccat atcaaataga gatagaattt tttataaaca
     5401 aatcttgtga ggagattttt aatgaaaaat aaactacttc ccaatttaat catcattgct
     5461 cttgccgcta cgatatctgc gaactccata accgctcagg cgaaacaaaa tgatcaaata
     5521 caaaatagta atgaacaatt tacccaggat ccatttgata ctgatccttt ttttcaatca
     5581 catgatgatg ttctgctgca aatacacaaa atgcaacaag ctatggatca atttattaaa
     5641 agtcaatttt cacaaatgca aaataccctt gcaaacgatc ctcatcacaa tccctttggt
     5701 agtgcaaaaa atattcaaat tacagagaat aaagatgagc ttatttataa gataaaacta
     5761 ccgcaaggtt ctgatagtaa agtggatatt accgttaaaa acgggcagtt agttcttagc
     5821 gccaacgtca ctcagaaaat cacccgtgaa caagataata acaaaagtgt cagtttctcc
     5881 caaagtaatt acatgcaatc atttccattg ccccagagct atgatccgaa gtccatggtt
     5941 acaaaaatga aagattcaaa tttaattgtt acttttaaaa aacattctta accgcattat
     6001 ctgcttaatt aaataacctg ctaaaacaac catgggaaaa gattgattgc acagtttgat
     6061 accagcttga aattttgatt ttctgctgca ttcgtcacag gaaatcaaga gccagggttt
     6121 gaatttttta ttgcaatgat tccctgtctt tgcacataaa attcaagcac tgactctatt
     6181 tttcccagct ataaaattaa ctgtttattt taactaggac ttagctaagt tagattgaac
     6241 cgaatttact ggaagcactt cattgcctgt tattaacttt atgattttat ttattaattt
     6301 tttttggggc ttgttgaaaa aagagagcat ggcccctgtt ttatttatgt tagttatagg
     6361 atctaatctg caaaccagat aagatatact gtctgtcaca tccgattaga acaattatta
     6421 aacaatattc taatctgata atcatttgaa tggagttctg aattaattta aaaaggagtt
     6481 tg
//
LOCUS       DATNSN010000163         6469 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.163, whole genome shotgun sequence.
ACCESSION   DATNSN010000163 DATNSN010000000
VERSION     DATNSN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.163"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1266
                     /locus_tag="VAH76_002646"
     CDS             <1..1266
                     /locus_tag="VAH76_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEO1387236.1"
                     /translation="KLDFNGFEPIIKSYTHDNQKYYFAYQPISSTNEKALWHIIIIVP
                     ETDVIEPLKDLSMRYILLTILVLLIGTLLVRIVSQRISNPIIQLTEEAKEITMLNLKP
                     RPLLKTRIKEVSYMDKTLSSLRSSLASFQRYVPGSLVKKLMRSGKIAQVGGQSQTITI
                     LFSDIKDFTSISESTSPQKLMTYLSDYFQSMTEIVIQNEGTLDKYIGDAIMALWNAPS
                     KDTNHALHACLTAKIMIERLSLLNQKNKHLGFPEFKIRIGIHTGDAVVGNVGSEDRLS
                     YTALGDSVNLASRLESINKNYHTQIIVSHATFTHVSEKFPFRLLDEVAVRGKRESIEI
                     YELITAQNLENLERHKKEFQKAFSLYQKGSWKESISAFAKLTPAYSGDPLASVYIERC
                     KVLAVNPPVNWDGIWREGKTDYSLLGKFD"
     gene            complement(1287..2315)
                     /locus_tag="VAH76_002647"
     CDS             complement(1287..2315)
                     /locus_tag="VAH76_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO1387237.1"
                     /translation="MDTATIKWGILGTSFISEVMANAIQESKTSELVAIGSRTSEIAK
                     RFSEKFAIPKYYDNYQSLILDSDIDVVYIGLPNHLHKEWIIRCAQAGKNILCEKPFVI
                     GIEEIHEVISVIERTNVFCMEALMYRYHPFIKKLQEIIQSNTIGKIMLYNATYTANIA
                     KIANPVAGGSIRNLGCYPISLVRLLANAEPIEIRGIGRMNSRNNTDSQASVLLKFEDD
                     SIAAVSTADDIEMHWQFEVYGTKGHLKVDTNPWLPGCEGNKIYLSLNTESTPREISVK
                     AEKSLYTYQIDCINEQVLKEDSRQFSKMSLLDSMGNTIVLETWLKQILPSKPSLELSR
                     LKDRLPHA"
     gene            2488..4863
                     /gene="lspD"
                     /locus_tag="VAH76_002648"
                     /gene_synonym="gspD"
     CDS             2488..4863
                     /gene="lspD"
                     /locus_tag="VAH76_002648"
                     /gene_synonym="gspD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspD family T2SS secretin variant LspD"
                     /protein_id="HEO1387238.1"
                     /translation="MRSIVIILLAFVCCSLVRANDVNISTVTKDGNTFFYLQAGAYNL
                     EKDAKIRQNELAKLVDQKVEIKNLADKHLYLVQIGPIDDYQTARALKEKLSQKTEKQF
                     NKISKNQTSSLEVSQLPKNMEQAQTAPPGSKLWNLRNADIRAVIAEVSRITGKNFVID
                     PRVQGKVSIVSSTPLSSRELYQVFLSVLQVSGYAAIPNGEIIKIIPNIDAKTQSPDLL
                     SGMKSPPRGDDMMVAVIPVHYVPSEQLVPVLRPLMPQWSSVSAYAPSNMLILSGRANN
                     IKSLAEIIKQVDSSSANGIDMVRLHHALAMDVANTLKDLVKTQPGIGSRTQITIAADD
                     RSNSILVSGSKTDRIRLRMLILKLDKDSSTGVNSNTQVVYLNYLRAEDLVPILAGIAQ
                     ANFSGNVGTTIGTITRPALDSTNPASSLVNTSADGQSSSLSSTSTSSGPMNASGATAN
                     TTSASTQNEGSTKPTVQIIGEPNTNSIILNAPASIIRILKTVISQLDIKPAQLLIEAL
                     VAEVDEKDVNNLGIEWGSNQQTGNPNDFRPGFAIINSKTRIDDFQAQIYALAREQRAN
                     ILSTPSVVVLDNRQAKILIGKQVSVASTSYPNNAGGTTTASPYITFARVNVALHLYVR
                     PQITRGQGIQLQIDQGNDTLDPSDAATENTTTPTFNISSIVTSVHVESGDIVVLGGLI
                     QDSIGNDNNKLPILGDIPGIGRLFQRNIRNRDKRVLMVFIKPIILRNERDNLHVTGEK
                     YNYIRQYQLDWIRSQEAFEQTDDETVLPPLTQGNLPVPFSTPPQYSSKMTK"
     gene            4867..6351
                     /gene="lspE"
                     /locus_tag="VAH76_002649"
                     /gene_synonym="gspE"
     CDS             4867..6351
                     /gene="lspE"
                     /locus_tag="VAH76_002649"
                     /gene_synonym="gspE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE family T2SS ATPase variant LspE"
                     /protein_id="HEO1387239.1"
                     /translation="MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFA
                     EIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSE
                     DLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
                     IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILD
                     KQAAQLDLNLLGMPEHTLKTVREMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTR
                     NILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIA
                     VQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKT
                     PHELRQEEKELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIH
                     RNENIQTMENYLRPATPSIREDGFKRVLCGDTSLAEILRVTSQN"
BASE COUNT         2120 a         1287 c         1175 g         1887 t
ORIGIN      
        1 aaactggatt tcaatggttt tgaacccatc atcaaatcgt atactcatga taatcaaaaa
       61 tattattttg cataccaacc tatatccagt acaaatgaaa aagcactgtg gcatattatt
      121 attattgttc ctgaaacgga tgtgatagaa ccactgaaag acttaagcat gcgctatatt
      181 ttgttaacta tactggtttt gctgattggg acattattag ttcgtatcgt ttcacaaaga
      241 atatcaaatc ctattattca actgactgaa gaagctaaag aaattaccat gcttaacctc
      301 aaacccaggc cacttctgaa gaccaggatt aaagaagtca gttatatgga taaaacacta
      361 tcatctctgc gatcaagctt agcttcattt caacgctatg ttcccggatc tttggtaaaa
      421 aaattaatgc gtagcggaaa aattgctcaa gttggaggac aaagccaaac aattactatt
      481 ttattttcag atataaaaga ttttacaagt atttcagaat ccacctcacc acaaaaatta
      541 atgacctatt tatccgatta tttccagtcc atgaccgaga tagtaataca aaatgaaggg
      601 actttggata aatacattgg cgatgctatc atggcattat ggaatgcacc ttcaaaagat
      661 accaatcatg cattgcatgc atgcctgact gccaaaatca tgattgagcg ccttagcctg
      721 ttaaatcaaa agaataaaca tcttggattt cccgaattta aaatcagaat tggcattcac
      781 accggagatg ctgttgtggg taatgttggt tcagaggaca ggctaagtta tacagccttg
      841 ggcgactcag tcaatctggc aagtcgcctg gaatcaatta ataaaaatta tcatactcaa
      901 atcattgtca gtcatgccac tttcactcac gtatctgaaa aatttccttt ccgtttgtta
      961 gatgaagtag ctgtaagggg taagcgtgaa agtattgaga tatatgaact gattacagct
     1021 caaaatcttg aaaatcttga gcgacataaa aaggaatttc aaaaagcatt ttctctctat
     1081 caaaaaggtt cctggaaaga gagtataagt gcttttgcaa aacttactcc agcctactct
     1141 ggtgatccat tagcttccgt ttacatcgaa cgctgcaagg tattagcagt taatccacct
     1201 gtcaattggg atggtatctg gcgcgaagga aaaacagatt actctcttct cggaaaattt
     1261 gactagcatc accagaacca ttttgcttat gcatgaggca gcctgtcttt taatctggat
     1321 aattcaaggc ttggttttga agggagtatt tgttttagcc aggtttccag aacgattgta
     1381 tttcccatgc tatctaataa agacatcttg ctaaattgtc tggaatcctc tttcaatact
     1441 tgctcattga tgcaatctat ttgataggta taaagtgact tttctgcttt cacgcttatt
     1501 tctctgggtg ttgattcagt attgagagag agataaattt tgttaccctc acagccaggc
     1561 agccaaggat tcgtgtctac ttttaaatgg ccttttgttc cataaacctc gaattgccaa
     1621 tgcatttcaa tatcatcggc agtggaaact gctgcaattg aatcatcttc aaattttaaa
     1681 agaacgctgg cttgactatc cgtgttattc ctggaattca ttcttccaat accacggatt
     1741 tcgattggct ctgcgttagc aagcagcctc actaaggaaa ttgggtaaca tccgagatta
     1801 cgtatacttc ctcccgcaac aggattggct atttttgcaa tgtttgctgt gtatgtcgca
     1861 ttatacaaca ttatcttacc tattgtgttg ctttgaatga tctcttgtaa tttttttata
     1921 aagggatgat atcggtacat tagagcttcc atacaaaaaa catttgttct ctcaattact
     1981 gaaataacct catggatttc ttctatacca ataacaaaag gtttttcaca caaaatattt
     2041 tttcctgcct gcgcacaacg aataatccat tctttatgca gatgattagg caaccctata
     2101 taaaccacat ctatatcact atcgagaatt aatgattgat aattgtcata atattttgga
     2161 atagcgaatt tttcagaaaa tcgtttggct atctcgcttg ttcgactccc aatagcaaca
     2221 agctcactgg tttttgattc ctgaattgca ttagccatca cttcagagat aaaacttgtt
     2281 cctaaaattc cccactttat tgtcgcagta tccatatatt ccttccggct aaaaaattgc
     2341 tgactgtata ttaaaacgca taaatgacac gagtaaaacc atagcagtaa aaaccactta
     2401 aatttcctgc tctaagttat taaaaaagct atggtaagca gtcaaattta gttattatgt
     2461 ctttatccat tattttacgg tttttaaatg cgctcgatag taattatact tttagccttc
     2521 gtttgttgtt cattagtacg tgctaatgat gtcaatattt caaccgtaac caaagatggc
     2581 aatacatttt tttatcttca ggctggggcc tataatctcg aaaaagacgc taaaatacgc
     2641 caaaatgaac ttgccaaatt agtcgatcaa aaagttgaaa ttaaaaacct ggctgacaaa
     2701 catctttacc ttgtgcagat tggtccaatt gatgattacc aaactgcaag agcactcaaa
     2761 gaaaagttat cgcaaaaaac agaaaaacag tttaataaaa ttagcaaaaa tcaaacctct
     2821 tcacttgagg taagccaatt gcctaaaaat atggaacaag cgcaaacggc tcctccaggc
     2881 agtaaattat ggaatttacg taacgctgat attagagccg taatcgcaga agtctctcga
     2941 attacaggta aaaattttgt tattgatccc agagtacaag gaaaagtatc gattgtttca
     3001 tcaacgccgt tatccagccg cgagctatat caagtctttc tctcagtact gcaggtatca
     3061 ggttatgccg caatacctaa cggagaaatt atcaaaatta ttcccaatat tgatgcaaaa
     3121 acacagtcac ctgatttact cagtggaatg aagagtcctc cacgaggtga cgatatgatg
     3181 gtcgccgtaa tacccgttca ttatgttccg tctgaacaac tggtacctgt tctaagacca
     3241 ctaatgcccc aatggagcag cgtatctgcc tatgctcctt ctaatatgtt gatcctgtct
     3301 ggtcgagcaa acaatattaa aagccttgcg gaaattatca aacaagtgga tagctcaagt
     3361 gcaaacggca tagacatggt tcgtttacat catgctttgg ctatggatgt tgcaaacact
     3421 ttaaaagacc tggttaaaac tcaacctggt atcggcagca gaacacaaat taccattgct
     3481 gcagatgacc gttccaactc aattttagtc agtgggagca agacggatag aattcgtttg
     3541 cgaatgctca tattgaaact ggataaagac agctcaacag gagtcaattc aaacacacaa
     3601 gttgtttacc tgaattattt aagagctgaa gatttagtcc ctatactggc aggtatagcc
     3661 caagccaatt tcagtggaaa tgtaggaaca accattggaa ccattacaag acccgcatta
     3721 gacagcacca accctgcatc cagtcttgtc aatacatctg ccgatggaca aagctcttca
     3781 ttaagctcca cttcgaccag ttctggccct atgaatgcgt caggagcaac agcaaatacg
     3841 acatcagcaa gtactcaaaa tgaaggctct accaagccta ctgtacaaat aataggagaa
     3901 ccgaacacta actctattat tcttaatgca cctgcttcaa ttattcgcat tctaaaaaca
     3961 gtgatttctc agctagatat aaaaccagct caactattaa ttgaggccct cgttgctgaa
     4021 gtggatgaaa aggacgttaa caatctggga atagagtggg gatcaaatca acaaacaggc
     4081 aatcccaatg atttcagacc aggctttgcc attataaaca gtaaaaccag gattgatgat
     4141 ttccaggctc aaatttatgc cttggcaaga gagcaaagag caaatatttt atcaacacct
     4201 tcagttgtag ttcttgacaa tcgtcaagca aaaatactca taggtaagca ggtttcagta
     4261 gcttcaacga gttaccccaa caatgctgga gggactacta ccgcaagccc ttatataaca
     4321 tttgccaggg ttaacgttgc tttgcatctc tatgtaaggc cacagattac tcgcggacaa
     4381 ggaattcaat tacaaataga tcaaggtaat gatactttag atccttcaga tgcagcaaca
     4441 gagaacacaa caacccccac ttttaatatt agcagtattg tgacatcagt tcacgttgaa
     4501 agtggtgaca ttgtagtgct cggaggatta attcaggaca gtattggaaa tgacaataat
     4561 aaattaccta ttttaggaga tatacctggc ataggcagat tattccaacg taacattcgt
     4621 aacagagaca aacgagtgct catggttttt attaaaccta tcatattacg caatgaaaga
     4681 gataacttgc atgtcactgg agaaaaatac aattatatcc gccaatatca actggattgg
     4741 attagatccc aagaagcttt tgagcaaaca gatgatgaaa cagtgttgcc cccattaact
     4801 caaggcaatc taccagtacc ttttagtact cctccccaat attcatcaaa aatgaccaaa
     4861 taaatcatgg aaaatgaaga aaaatcactt aaaattcctt atagttttgc caaaagcaat
     4921 ggaattgttg tgggagaaat acaagaaaat ctggctattg tctatcactt acctaacaca
     4981 ccactgcaag ctttcgctga gataaagcga ttattacaat gtgaacttga tctaaaacaa
     5041 gtcgatgaat ccacttttca gcaacattta gccaatattt accaatctaa atcttcaata
     5101 ctggatgctg cagaaggcat ggaagaagat atggatttat ccatgttggc cagtcaactt
     5161 cccgtcagtg aagacttatt ggaaaaccaa gacgatgccc caattatccg cttactgaac
     5221 gctctgttta cccaagcgat taaacaaaaa gcatctgata tacacattga aacttatgaa
     5281 gacagagtat tggtgcgcaa tcgaattgat ggtgttctac aagaggtcct tgaaattcaa
     5341 cgagctatcg ctcctttggt catttccaga gttaaggtga tggccaaatt ggatatcgcc
     5401 gaaaagagaa ttcctcaaga tggtcgaatt tcattgcgta taggaggaca taatattgat
     5461 gttagggtct caactttacc ctcaaaccat ggagaacgag tggttttaag gatattggat
     5521 aaacaagctg ctcaactgga tttaaatctt ttgggtatgc ctgaacatac attaaaaaca
     5581 gtacgagaaa tgattgctga accccatggc attattttag tcactggccc aacaggttca
     5641 ggcaagacaa cttctctcta tgctatgctc accgaactaa atcaggtgac tcgtaatatt
     5701 ttaaccattg aagatcccat tgaatatgac ctgcctggtg taggacaaac gcaggttaat
     5761 actaaagttc aaatgacttt tgccaaagga ttaagggcca tattacgtca agatccggat
     5821 gttgtcatga taggagaaat ccgtgatttg gaaacggctg aaattgctgt tcaagccagc
     5881 ttaacagggc atttggtact atcaaccctt cataccaata ccgcattagg agcattaact
     5941 cgcctgcatg atatgggagt tgagtctttt ttattgtcat ccagtatagt cggcctcatt
     6001 gcgcaacgtt tggtaaggaa attatgctct cattgcaaaa cacctcatga attaaggcag
     6061 gaagaaaagg agttaatggg tcttaaacct gatgctgatg tatctcaggt ctttgagccc
     6121 aagggatgcg actattgcaa ccacttgggt tataaaggca gaacaggaat ttatgaatta
     6181 attatcgtag atgaaaccct gagaggaatg attcatcgta atgaaaatat tcaaacaatg
     6241 gaaaattatt tacgacccgc cacgccgagc atacgtgaag atggttttaa aagagtatta
     6301 tgtggcgata cgtcacttgc agaaatttta agggtaacat cccaaaatta attcttttcc
     6361 cagtacaaag actaatgtta agtcctacta tacacgttat ttaacttctg aattctatac
     6421 tgacttaaac ttccaaaaaa tcataatgat ctcaagtacg cttaaattt
//
LOCUS       DATNSN010000164         6411 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.164, whole genome shotgun sequence.
ACCESSION   DATNSN010000164 DATNSN010000000
VERSION     DATNSN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.164"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            62..589
                     /locus_tag="VAH76_002650"
     CDS             62..589
                     /locus_tag="VAH76_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEO1387240.1"
                     /translation="MNRIKLGISNLIFMGRWLQLPLYLGLILILAGYVYRFIIELFEL
                     IFHLKGLDDTHIMLGVLDLIDVVMIANLLIMVVMGGYETFVSHLQLDSHPDQPEWLDH
                     FDAGAMKIKLALSLIGISSIHLLRTFIDPAKLSNYSIMWQVVIHLTLIVSALAIALTN
                     RLMDQPARKNLQHPD"
     gene            827..2044
                     /gene="legC2"
                     /locus_tag="VAH76_002651"
     CDS             827..2044
                     /gene="legC2"
                     /locus_tag="VAH76_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC2/YlfB"
                     /protein_id="HEO1387241.1"
                     /translation="MTDTPKANLSVDEIKEDTPKNQPLPEENTEILVKKLKVLETTQE
                     NFDQIKKHIAAVIDSLAKNRSFFGRVAAYWGEMQLWLKITLGIVLVVPTLTLGIAIEV
                     ASLIVVSVLTLVTYVGSALLLENHVQKEEHITDRLKEEMIGLADNLGKVIESFEPLRK
                     QIAEQIEYFHKENDRLNLNVTQLSDHIMRLTKQAEQLQKTEQTLRKVREELEKTSETL
                     DEKVQVQTELLQINQEELNRIREEIAKNEIELSEKISELDQVKKEMSVEIEKAETVTQ
                     VLKNTVEKLSQTAIADHNHRESFQNKLNDFLSNKEQSFNDVAQRICEAERELCLVKEE
                     LNFSNKRYQELLERQEEVVKRLEQLQLSRKPTPYSNAKVLGKLGIMATEEKESPTPVS
                     SEENTIPTQTLTG"
     gene            2367..2681
                     /locus_tag="VAH76_002652"
     CDS             2367..2681
                     /locus_tag="VAH76_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387242.1"
                     /translation="MSTPKWNLSISRAIIFVIALFASMICARAHFVGGIHTNAGYVGW
                     YHPDYYNHGVISVGNYHPGYGWVAPVYATPTYIIGNTGYDCQTVQQCDSEGNCIQSQN
                     CD"
     gene            complement(2847..3956)
                     /locus_tag="VAH76_002653"
     CDS             complement(2847..3956)
                     /locus_tag="VAH76_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387243.1"
                     /translation="MELKRQCINGVAGVSLSLLAVLIHPLFAEDAPQFVKGQSQLPAA
                     QDPADSSWLPDIIGLPNEEQVESLTRHVAEVMTEEEDKNCTAIDYNLRMLVFQNFVIY
                     QHMANAQHHYFKEKMVLKYAHVLAMILKESSGDPTNVTDMNGRSISTSKPGTNLQRWS
                     NLLKLTMQNGIKFNYQTNFGLTQLSADRLFIAFKLANGASHSKDFLEGKNGDETPNRV
                     DMDSAIAIRRLIWFYQDIAQGRLKQKDECINEEDIYKPEFNERYQAGLRMALLYCGTK
                     FLFRVDNEPVWVNENSTFEKAMASIAYCKLGNSKTGYGTDEMDEQCFAEWVTLCPALN
                     VNIALLTPLSYFQTRKNKPVCTDTFNRLLNKEPKN"
     gene            complement(4038..4313)
                     /locus_tag="VAH76_002654"
     CDS             complement(4038..4313)
                     /locus_tag="VAH76_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO1387244.1"
                     /translation="MTKELCMRCYISGRVQGVWFRASAKNLAEQLMISGWARNLADGR
                     VEVFACGKEDKLEEFYTWLQKGPLNARVDVCTRENLPWEDYITFDVL"
     gene            4470..4823
                     /locus_tag="VAH76_002655"
     CDS             4470..4823
                     /locus_tag="VAH76_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387245.1"
                     /translation="MKKVILYALPSLMLAILFSPAFADETPAQLTGTPAEQIQQLNAQ
                     IQSQLQEMQTKQQQQLDALNTQLQNQIKQVQSQLQEQIQTVNSQTQDQIKQIQATLQE
                     QIKQVQQQALQKANQ"
     gene            complement(5004..6335)
                     /locus_tag="VAH76_002656"
     CDS             complement(5004..6335)
                     /locus_tag="VAH76_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1888 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEO1387246.1"
                     /translation="MLQFFEKGLQYAGENISADSYLMRKIVEAAIPSADKGTVSAEKM
                     LGLVNPNQIEIVSGSESAYRLIVHKIKEAKKQILIQAFVWEPGTQVVKDIRNALCDIK
                     HEVEVFLLVDQLDRLAQVFYHGEILPKKPKHDPACLGLDNLPQNIKLHIVTHVHNSIA
                     SNHNKAIWIDGDVILTGANFQQENYGPNGFHDAAMFIPGGAAEAVFYDFKAMWDKRTN
                     KSDAPEISPIYTGQESIESYRNTNSCSVLYVTNTIRQKWALLPFYRAPLPEDPLNNAY
                     MAAIKYAKHTIRIAVPNLNSPEIIHALADFINNRNGHVYLLMGKGFNDFRERFYGGTN
                     QSSIDYLSSLLDEDRQNNLQVRWFHRVNDNEISIKKEVIHMKFMSIDGQVVIYGSSNL
                     DLLSLHNSHETNIVIDNADFAQRAIEQLYLPIFETSIPVLISPKRTCFVCS"
BASE COUNT         2122 a         1214 c         1073 g         2002 t
ORIGIN      
        1 ccttcaggaa agcttatcat aaggtcattc aataagttat attttgtagt gaatcgacaa
       61 aatgaatcga atcaaacttg gcatcagtaa tcttatattt atgggaagat ggcttcaact
      121 tcccttgtat cttggtttga tcttgatact ggccggatat gtttatcgct tcatcattga
      181 attatttgaa ctcatttttc atcttaaagg cttggatgat acccatatca tgcttggagt
      241 attggatctc attgacgtcg tcatgattgc caatcttctg attatggtag ttatgggcgg
      301 ctatgaaacc tttgtatccc atttgcaatt ggattcccac cctgatcaac ctgaatggct
      361 tgaccatttt gatgcaggag ctatgaaaat caaattggct ctttcactga taggtatctc
      421 ctcgattcat ttgttaagaa cttttataga ccctgcaaaa cttagcaatt attctattat
      481 gtggcaggtt gtgattcatt taactttaat tgtttcagcc ttggccattg cattaaccaa
      541 tcggttgatg gatcagcccg ccaggaaaaa cctccaacac cctgattgat ttaaaaatct
      601 gaaaattaaa taacttcttt ctctgatgtc actcactctg cacatgacaa aaacttgtta
      661 tgtacagagt cccgctctaa aaaaataatg actggcttaa aattattcga tagaatcagt
      721 aatgtttgac ctattcaggg aactcatggg attgaaccag aaagaagtag attgcaagct
      781 ctttcgggtt gaatgtagtc aaaatggaaa taaaggaata tgaatcatga cagatactcc
      841 aaaagctaat ctatccgttg atgaaattaa agaggatacc cccaaaaacc agcccctccc
      901 tgaagaaaac acggaaatat tggtaaaaaa actgaaagta ttggaaacta cccaagaaaa
      961 ttttgatcaa atcaaaaaac atattgctgc tgttatagat tccttggcaa aaaatcgcag
     1021 tttttttggc cgtgttgctg cctactgggg agaaatgcag ttatggttaa aaattactct
     1081 tggaatagta ttggttgttc ccaccttgac acttggaatt gcaattgaag tggcctctct
     1141 tattgtagtt agtgtcctga ctctggtgac ttatgttggc agtgccctgc ttcttgaaaa
     1201 tcatgttcaa aaagaagagc atattactga tcgtttaaaa gaagaaatga taggtttggc
     1261 agataacctg ggaaaagtta tcgagtcttt tgagcctctc cgcaagcaaa ttgctgaaca
     1321 aattgagtat tttcataaag aaaatgatag attaaacctc aacgttactc aactgagtga
     1381 tcatattatg cgattaacaa aacaagctga gcaattacaa aaaaccgaac aaactttgcg
     1441 taaagtcaga gaagaattgg aaaaaacatc tgaaactctg gatgaaaaag tacaagttca
     1501 aactgaatta ctacagataa atcaggaaga attaaacaga ataagggaag aaattgcaaa
     1561 aaatgaaatt gaattatctg aaaaaatttc tgagctggat caagttaaga aagaaatgtc
     1621 agtagaaatt gaaaaagccg aaactgttac ccaagtatta aaaaatactg tagaaaaact
     1681 gtcgcaaact gctattgcag atcacaatca tagggaatcg tttcaaaata aattgaacga
     1741 ttttctctct aataaagaac aaagtttcaa tgatgttgct caacgtatat gtgaagccga
     1801 gcgcgaacta tgccttgtta aagaagaatt aaactttagt aacaaacgtt atcaggaact
     1861 attggaacgt caagaagaag tagttaaacg tttggaacaa ctccaacttt cccgtaagcc
     1921 tacgccatat agtaatgcaa aagtacttgg taaattaggt attatggcta ctgaggaaaa
     1981 agagtctcca acacccgttt catcagaaga aaatacaatc ccaactcaaa ctctcacagg
     2041 ttagatgttt ataaatcgat gtgctatggc acatcgattg cctatcacgt gtggtcatcc
     2101 aaaaaccaat cagttcaacg aaatttattt taaaacagtt cgccaattat atactgagca
     2161 taaataggtt caaactaaag caattcgtag acagcataat tgttctacta atgagctact
     2221 ttagatctat tgcccataat caatttatat taaatcagtt aattttttta ctttatgaac
     2281 acaaacccac tgagaacaat aaaagggcta tacttttaga atattgactg ttattagtga
     2341 aatgattctt tgataaggag ataactatga gcacacccaa atggaattta tctatttctc
     2401 gcgcaattat cttcgtcatt gcattatttg cttctatgat ttgtgctaga gctcatttcg
     2461 ttggtggaat tcatacaaat gcaggttatg tgggatggta tcatccagat tattacaatc
     2521 acggtgttat aagcgtgggt aattatcacc caggttatgg ctgggttgct ccagtgtatg
     2581 ctacgccaac atatataata ggaaatacgg ggtatgactg ccagacagtc cagcaatgtg
     2641 attcagaggg aaattgcatt caaagccaaa actgtgatta gtagtggaaa atgtagcata
     2701 tcaatgcgca ttatcttttt tactagattg agaacaaatt tccctccact taggctctct
     2761 aatcatcaaa actgttaatt agattaatag gtaaccagca agtaacgata ggattttaaa
     2821 tgttcaaagt gataaggcaa aggctttcaa ttttttggtt ctttattcag taaacgatta
     2881 aatgtatcgg tacaaactgg tttatttttt ctggtttgaa aataactaag cggagtaagc
     2941 agtgctatat tcacattcaa agctggacat aaggttaccc attctgcaaa acactgctca
     3001 tccatttcat cggtaccata ccctgttttt gaattgccta gtttgcaata ggcaatagaa
     3061 gccattgcct tttcaaaggt cgaattttca tttacccaaa caggttcatt atcaacgcga
     3121 aacaaaaatt ttgtaccaca atataaaaga gccattctta agccagcttg gtatctttca
     3181 ttaaattcag gtttatagat atcctcctca ttaatacatt catctttttg ctttaatcgc
     3241 ccttgagcaa tatcctgata aaaccaaatc aatctgcgaa tggcaatagc cgaatccatg
     3301 tctactctgt taggagtttc atctccattc ttcccttcca gaaaatcttt gctatgactc
     3361 gctccattag ccaatttaaa agcaataaac aacctgtctg cagagagctg agtcagccca
     3421 aaattggttt gataattaaa tttaattcca ttttgcattg ttagttttaa taaattgctc
     3481 cagcgctgca aattagtgcc aggtttgctt gtagatattg atcggccatt catgtctgtt
     3541 acattagtag gatcgccgct actttctttt agaatcatgg caagaacgtg agcatatttc
     3601 agtaccattt tttctttaaa ataatggtgc tgtgcattcg ccatatgctg ataaattaca
     3661 aaattttgaa aaacaagcat cctgagatta tagtctattg cagtacaatt cttatcttcc
     3721 tcttctgtca ttacctcagc gacatgtctg gtaagacttt caacttgctc ttcattgggc
     3781 aagccaataa tatcaggaag ccaactactg tctgccggat cttgtgcagc tggtaactgg
     3841 gattgacctt taacaaactg tggggcatct tcagcaaaga gtggatggat taacacagcc
     3901 aataaagata aagaaacacc tgctaccccg ttaatacact gtctttttaa ttccataagc
     3961 tgtaccttct ttattgagta tagcttgtat ctattcgaag gcatggcaag taaaaattaa
     4021 attacaattg gcttttttta taagacatca aaagtgatat aatcttccca aggtaaattc
     4081 tctcgtgtac atacatcaac tcttgcatta agaggtcctt tttgcagcca ggtataaaat
     4141 tcttccagct tatcttcttt tccacatgca aaaacctcaa cacgaccatc cgctaaattc
     4201 cgagcccatc cacttatcat caattgttca gccaaatttt tagcagaagc tctaaaccat
     4261 actccttgaa ctcttcctga aatataacaa cgcatacata actccttggt cataattctt
     4321 ttccacctat cgtaaaaata tcagttactt catttcattt gaccttgcat aacctgtttt
     4381 cactctgatt ctgctatagt taaattagca ttcttattag agcaaaataa attagataat
     4441 ttggactttt tcaataatgg agataaaaaa tgaaaaaagt gatattgtat gcacttccat
     4501 ctttaatgtt ggcaatcttg ttttccccag cctttgctga tgaaacccct gctcaactga
     4561 ccggcacacc agctgaacag attcagcaat taaatgctca gattcaatct caattacagg
     4621 aaatgcaaac aaaacaacag caacaactgg atgctttaaa tacccaatta caaaatcaaa
     4681 ttaaacaagt ccaatctcaa ttacaagaac aaattcaaac ggtcaattct caaacacaag
     4741 atcagattaa acaaatacaa gcaaccctac aggagcagat taaacaagta cagcagcaag
     4801 cattgcaaaa ggctaaccaa tagttatttg aatttatata atttctccca tgtaggcctg
     4861 tgtattgaca atgcttacgc atctaaaact taaagacatt gatggtaatt tgcatgagtt
     4921 tggataaaaa tggaattatt ttttatgtcg aactcatgta tcatgattat aattaatatt
     4981 ggcttgatca aaacaaccta aaatcaagaa caaacaaaac aggttctttt aggcgatatc
     5041 aatactggta tactcgtttc aaagatgggt agatataatt gctctattgc tctttgtgca
     5101 aaatcagcat tatcaattac aatatttgtt tcatggctat tatgtaaact taacaaatca
     5161 agattcgatg aaccgtaaat aaccacctgc ccatcaattg acataaattt catatgaata
     5221 acctcttttt ttatagaaat ttcattgtca ttcacacgat gaaaccaacg aacttgcaag
     5281 ttattttgcc tgtcctcatc aagtagtgaa ctaagataat ctatcgaact ctgatttgta
     5341 ccaccataaa atctctcgcg aaaatcattg aatccctttc ccatcaatag atagacatgg
     5401 ccatttctat tattaataaa atccgccagg gcatggatga tttccggcga gtttaaattg
     5461 ggaacagcga ttcggatagt atgtttagca tacttaatag cagccatgta ggcgttattc
     5521 aatggatctt caggtaaagg cgccctataa aaaggtaaaa gcgcccattt ctgcctgata
     5581 gtatttgtca catagagcac agaacaagag tttgtatttc tataagattc tattgactcc
     5641 tggcctgtat atatgggaga aatttctggt gcatcagatt tattggtgcg cttatcccac
     5701 attgctttaa aatcgtaaaa aactgcttcc gcagcgccgc caggaataaa catggcggca
     5761 tcatgaaacc cattagggcc ataattttcc tgctgaaaat tagcccctgt caaaatgaca
     5821 tccccatcaa tccagatggc tttattatgg ttggacgcga tggaattatg aacatgagta
     5881 actatatgca atttaatatt ctggggtaaa ttatctaaac ctaaacaggc tggatcatgt
     5941 tttggttttt taggaagtat ttcaccatga taaaaaactt gtgccaaacg atccaactga
     6001 tcgaccagaa gaaatacttc gacttcatgt ttgatgtcac aaagcgcatt tcggatatct
     6061 ttgacaacct gagttcctgg ttcccataca aaagcctgaa taagtatttg ctttttagcc
     6121 tctttgattt tatgaactat taaacgatat gcgctctcac tacccgatac tatttctatc
     6181 tgatttggat tcaccaggcc cagcattttt tctgctgaaa cagttccctt atcagcagag
     6241 ggaattgctg cttcaactat ttttctcatt aaataagaat cggcggaaat attttctcct
     6301 gcatattgca aacctttctc aaaaaattgg agcatgattc actcataaat taattttacc
     6361 taatttatat cattgaattg atttaatatt tttgcaaatt aatttgagca a
//
LOCUS       DATNSN010000165         6392 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.165, whole genome shotgun sequence.
ACCESSION   DATNSN010000165 DATNSN010000000
VERSION     DATNSN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.165"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(144..566)
                     /locus_tag="VAH76_002657"
     CDS             complement(144..566)
                     /locus_tag="VAH76_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HEO1387247.1"
                     /translation="MNIMIKVIVALVGVFFLPSAMASSPTTTDIDASKDISSLVFQVI
                     QNQMVLDSSTVESATIVLPQTPTDSYGVRIKLKTIAATKFGQMTANGIGKQGSFILNG
                     MVISNPIIRSRIGDEFIVTGFNKKQANQFIESVMTKNN"
     gene            complement(630..926)
                     /locus_tag="VAH76_002658"
     CDS             complement(630..926)
                     /locus_tag="VAH76_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mitomycin resistance protein mcrB"
                     /protein_id="HEO1387248.1"
                     /translation="MTKNKFKATHPLLSLMNVGEATYKDLQLLGINSIHQLANASADQ
                     LYARLQQITGQLHDPCVWDVFAAAINEARTGEKQPWWQWTKIRKKRQLEGTFCI"
     gene            complement(1027..2352)
                     /locus_tag="VAH76_002659"
     CDS             complement(1027..2352)
                     /locus_tag="VAH76_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO1387249.1"
                     /translation="MSALYYNLNNHYVFHFIYSPEKRANRDNNPEKIMKKPLDTTEQN
                     RIAALYELKILDTPADERFDRIIRLTSGYLSVPIAFISFIDSKHQWFKSSCGIGLNKI
                     SRNDSFCHYTIQQNKPMIIPDALLDERFCNNPYVTNSPNIRFYAGFPLSTFDGFNVGT
                     LCAIDKKPRNLDETEIQILKDLATLAEDQVNLLDISLLEKTVREKNISLTKAKKELEM
                     RNNFIRKVFGSFMSDEIVAALLESKSKLKLGGEERKITILFSDLRNFTTLSEHFPADK
                     IVAALNNHYGKMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQAT
                     MQEVNTINRNSSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASRIQELTL
                     GGQILISEATYQEVNHSLELNGHLRVKVKGVAAPITIYDVIGLNKKSDS"
     gene            complement(2517..3542)
                     /locus_tag="VAH76_002660"
     CDS             complement(2517..3542)
                     /locus_tag="VAH76_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387250.1"
                     /translation="MRRSKAMQIPKKRTQILTISESSKEDLDVVPEYSDELDVSPNKE
                     TSKTNSKKLESTARKEQIKKLEGELYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVFEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRDGVMLAIELLEEADTKLLSNSEIIRRIGDDPGMAGGRWAKCKKVKPGERGVVVVKC
                     EGVGSCKYPYTLGFREGEEGVSPFDVLFELTRSVKVLNKNGSCKTIYHYSVPTPKEKE
                     LTNIVEDAENGIDEIHSDEFLSVVSSSPSSRFGFFTNKELSGISGLVNKAPEHIIPPS
                     DNTFSHH"
     gene            complement(3752..6277)
                     /gene="lgt3"
                     /locus_tag="VAH76_002661"
                     /gene_synonym="legC5"
     CDS             complement(3752..6277)
                     /gene="lgt3"
                     /locus_tag="VAH76_002661"
                     /gene_synonym="legC5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     Lgt3/LegC5"
                     /protein_id="HEO1387251.1"
                     /translation="MKEQQKAIFIENIKSTCAVDEQMAKELADSLLEIHQLNTGVNEQ
                     TLINEVFLTKFALYLKIGKEVALDWVRKNKDLDTPFPSDAVENQPAKDAKKPVENKAQ
                     VYGAMTVGRSPVNKALNQLTNDLLKEVVERGKTQKAQKLRAYIFDQLARRLEASLSQE
                     QINDLYNRIRGTGDYTKSESFSEEQLKILKEKVVPELKRELSDLSNGNVNILGLDVSR
                     EDKYAFDTTNIFSVWFSNNPAVYMPQHVKTQVEKTAKLNQPGKTRIVFSSLCLNETAQ
                     IDFQQWAKENNIELVDIDSIDLKSVSETDAQLLNLAKDELEAMRKGKGGNPAAASDLV
                     RWVDVIIGESSTYIDIDLPMNDKKVTVEVHSGFPVLLNMGSALTKDGQQPAMENPAFN
                     TDMIAYSKDKEARRQIIEGVAKKIIARYENCAKYIEESKNEELVRLKNSPGYKLFIEK
                     TDGKFDLCALRAAVSEAHQDALSFATFFGAEYFAKTFATQELIPVIKEAIQHQNQDLL
                     TSVIENHIEKQHLNDYPKTPDGIKKLLKSFQGIVYKPLVMEFSGPSAVSSSWVEAISG
                     RSIPRNFEYLAEPMSQPLRVLQHHACVSGKANFSSDNIPKWCELQSDVEKRQQQREDG
                     LSWLPDAQEKLKREGQQAKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQQI
                     KLEEEQQIKLEEEHKSKKYFAQSDAIDLILNNFENEINSIGAYYAPAKQRAIELLDTL
                     RKYKEEAFNDPSREKLISFAQNTKRAIQEATPILQKDLGWGDYLTNLAKQLVNAVTFA
                     VAYAVTFGTTGHQGFFALKSSLAVSQSQNLVEALNSELSQKNC"
BASE COUNT         1840 a         1288 c         1060 g         2204 t
ORIGIN      
        1 tgctgggaac aatgcactaa agacgatgat tgtggtgttg atcaaagctg tatagaaaca
       61 gcttgcggaa atcgatgtgt tacaaaatct gcagactaac taacctgggc aaattagccc
      121 ataaattttt cggtacatta atttcaatta ttttttgtca tcacgctttc aataaattga
      181 ttggcctgtt tcttattaaa ccctgttaca atgaattcat ctccaattct gcttcgaatt
      241 attggattcg atatcaccat accatttaaa atgaaactcc cctgtttccc tataccgttc
      301 gcagtcattt gcccaaattt ggtggctgca atagttttta atttaatacg cacaccatag
      361 ctgtctgttg gagtttgagg taatactatt gttgccgatt caactgttga gctatccaaa
      421 accatctggt tttgaatcac ttgaaatacc agtgatgaaa tatccttact ggcatcaata
      481 tcagttgttg tgggactaga ggccattgct gatggtaaaa aaaatactcc aacaagcgcg
      541 actattactt taatcatgat attcatataa gaattattgg gaaagagtaa aaaggtatat
      601 tatatcccgt gtttgcagta ttgaatactt taaatacaaa atgttccttc tagttgtctc
      661 ttttttcgta tttttgtcca ttgccaccaa ggttgttttt cacctgtgcg tgcctcgttt
      721 atagcagcag caaacacatc ccaaacacaa ggatcatgta attggccagt aatttgttgc
      781 aggcgtgcgt acagttgatc ggcacttgca ttagcaagtt gatgaattga attaatccca
      841 agcaattgca aatccttata agttgcctcc cccacattca taagacttaa aagtgggtgg
      901 gttgccttga acttattttt ggtcatatct atcccttagg ctgcttattt caatcataat
      961 accattgaga ttggtaattt aagcaatttc ttgatcccat gattttaaag agtgatatta
     1021 ttacttctat gaatctgatt ttttattcaa accgattaca tcataaatag tgataggagc
     1081 agcaactccc ttaactttta ctctgagatg cccatttaac tcaagcgaat gattaacttc
     1141 ctggtaagtt gcctcagaaa ttaaaatttg cccccccaaa gtcagctctt ggatacgtga
     1201 tgccagatta accggagaac caatcgcgct gtattgcata cgtttctgag aaccaatgtt
     1261 acctacaata gcataacctg tattaatccc tatgcccatt gccaattcgg gtaaactgct
     1321 gttgcgattt attgtgttca cctcttgcat ggtcgcctgc atctctaatg cacaggctat
     1381 cgctctgtat gcgtcatctc ctccggagtg tggtgctcca aataccacca taatggcgtc
     1441 accaataaag gaatcaacag taccattgta tttttctatg acatcaacca tttttccata
     1501 atgattatta agtgcagcga caattttatc cgcaggaaaa tgctccgaca gagtagtaaa
     1561 atttcttaag tcgctaaaca aaattgtaat tttgcgctcc tcccccccta attttaactt
     1621 tgattttgat tcaagaagcg ctgcaacaat ctcgtcagac ataaagctgc caaatacttt
     1681 acgaataaaa ttattacgca tttcgagttc tttctttgct tttgtcagag aaatattttt
     1741 ttctcgtaca gtcttctcaa gaagcgaaat atccagcagg ttgacttgat cttctgccaa
     1801 agttgccaaa tcttttaaaa tttgaatttc agtctcatct aaattacgtg gttttttatc
     1861 tattgcgcat agtgttccta cgttaaaacc atcaaatgtt gataacggaa atcctgcgta
     1921 aaaacgaata tttggtgaat tagtcacata aggattatta caaaatcgct catccaacaa
     1981 ggcatcagga ataatcatcg gtttattttg ctgaatagtg tagtggcaaa atgaatcatt
     2041 ccgggatatt ttgttaagac ctatgccaca gctggatttg aaccattggt gtttagaatc
     2101 aataaaagaa ataaaagcaa taggaacaga taagtaaccc gaagtgagtc ggataattcg
     2161 atcaaatctt tcatctgctg gtgtatccag aattttcaat tcgtatagcg cagcaatgcg
     2221 attctgctca gtagtatcca aaggtttctt cataattttc tcaggattgt tatcacgatt
     2281 ggctcttttc tcaggtgaat aaataaaatg aaagacatag tggttattaa gattatagta
     2341 caatgcgctc atattaattg gcttaaaaca acgaataagt taattgtatt cagcaatgga
     2401 aataaagttg atgagttact tgtatgtggc taacgcttgt tatggagggg atacgcagaa
     2461 acgggcaata aatagtattt aattgcccgt ctaaagcctt aaaaatttaa cctgatttaa
     2521 tgatgcgaga aggtattatc agagggtggt attatatgtt ccggtgcctt atttaccaat
     2581 cctgaaatac ccgagagctc cttgtttgta aagaaaccaa agcgtgatga aggagaactt
     2641 gaaacaactg acaaaaattc atcactatga atctcgtcta taccattttc agcatcttcc
     2701 acaatatttg taagctcttt ttcttttggt gtaggaacag aataatgata aatagtttta
     2761 caactgccat tcttgttcag tacttttaca gagcgcgtta actcaaatag tacatcaaaa
     2821 ggacttacac cctcttcccc ttctctaaaa cctaaggtat atggatattt acatgagcca
     2881 acaccttcgc acttgactac tactacccct ctttcgcctg gttttacttt cttacacttc
     2941 gcccatcggc ctccagccat tccaggatca tctccgatcc ttcttataat ttcactatta
     3001 gataatagtt tggtatcagc ttcctcgagc aattctatcg caagcatcac tccatcacgc
     3061 cttgtacctt tattgaaata tttttgagca tatttaagat caaaagcaaa aaaagtttcc
     3121 ccttttgctt tagttgatat cttttcgtcc tcttcttcaa atactttttt cttgttacca
     3181 aatattctct tcccttgatt taaattttca agaattgcca aatattcttt ttcactgaca
     3241 aagcgaaata aataattgct aggtcttcca gtgttaatgc tgaattctgc aaaagattct
     3301 tcttctaatt cgatagcatc ttgaatatga ctgtaaagct ctccttccaa tttcttgatc
     3361 tgttctttac gggcagtact ctccagtttt ttactgttag ttttgcttgt ttctttgttg
     3421 ggagaaacat ccagttcgtc agaatactcg ggtacaacat ccaaatcttc tttgcttgat
     3481 tctgaaatag ttaaaatttg tgttcttttt ttaggtattt gcattgcttt acttctacgc
     3541 atctaactct cctaacatcg ttacgatata caatatgtgc ttatattgtt cttgcacaat
     3601 ctattttgca cattatcagc aaaatgaaat ggattgcaat ctttcatttc taaatcaaaa
     3661 ttaccgaatc gaaatacatt agatttaaga agaatgcttg ggcagcaaaa ataatgtctt
     3721 gcccattagc actctatgtc tggtctattc cctaacagtt tttttgactc aattcgctat
     3781 tgagtgcctc tacaagattt tgagattgac taaccgccag agaagatttc aaagcaaaaa
     3841 agccttgatg cccggtggtt ccaaaagtta cagcataagc cacagcaaag gtcactgcat
     3901 taaccaattg tttcgctaaa ttggttaaat aatctcccca acctaaatct ttttgcagta
     3961 ttggagttgc ttcctgaatt gctctttttg tattttgagc aaaagagatt agtttttctc
     4021 tgcttggatc attaaatgct tcctctttat attttctcaa agtatctagc aactctatag
     4081 ctctttgttt tgcaggggca taataagcac caatagagtt aatttcattt tcaaaattat
     4141 tcaaaatgag atcaatagca tcggactgag caaagtattt tttgcttttg tgctcttcct
     4201 caagtttgat ttgttgctct tcctcaagtt tgatttgttg ctcttcctca agtttgattc
     4261 gttgttcttc ctcaagcttg attcgttgtt cttcttcaag cttgattcgt tgttcctctt
     4321 caagcttgat tcgttgttct tcctcaagtt tggcttgctg cccttccctc ttaagcttct
     4381 cttgagcatc aggaagccag gaaagtccat cttctctttg ttgttgtcgt ttttctacat
     4441 cagactgaag ttcacaccat ttaggaatgt tatcagaaga aaaattagct tttccagata
     4501 cacaggcatg atgctgtaaa actcgtaatg gttgagacat aggttcagct aaatattcaa
     4561 aattcctcgg gatggatctt ccagatatag cttccaccca agatgatgag acggcacttg
     4621 ggcctgagaa ttccataacc aatggtttat agacaatccc ttgaaacgac ttcaatagtt
     4681 ttttaatgcc atcgggagtc ttgggatagt cattgagatg ttgtttttct atatgatttt
     4741 caataacaga tgtaagcaaa tcttgatttt gatgctgaat tgcctcttta attacaggta
     4801 taagttcttg tgtagcaaaa gttttagcaa aatactcagc gccaaaaaaa gttgcaaacg
     4861 acaaagcatc ctggtgagct tctgaaaccg ccgcccttaa agcacataaa tcaaatttgc
     4921 catctgtttt ttcaataaat aatttataac ctggcgaatt ttttaaacgt actaactctt
     4981 cgtttttgct ttcctcaata tatttcgcgc agttttcata acgcgcaatg atctttttgg
     5041 caaccccctc gattatttga cgacgtgcct ctttatcttt gctataagca atcatatcag
     5101 tattgaaagc cgggttttcc atagcaggtt gttggccatc ctttgtgagc gctgacccca
     5161 tattaagtag aactgggaaa ccagaatgaa cctcaacagt gactttttta tcattcattg
     5221 gtaaatcaat atcaatataa gtactgcttt ctccaatgat tacatcaacc caacgcacca
     5281 gatcggatgc tgcagcagga tttcctccct tgccctttct cattgcttca agctcatctt
     5341 tggctagatt tagcaattga gcatccgttt cactgaccga ttttaaatct attgaatcta
     5401 tatcaaccaa ttcaatgtta ttttcctttg cccattgctg aaaatcaatc tgtgctgtct
     5461 cattcaagca taatgagcta aaaacaattc gagttttgcc tggctggtta agttttgcag
     5521 ttttttctac ttgagtttta acatgttgtg gcatgtaaac tgctgggtta ttgctaaacc
     5581 agacagaaaa tatattggtt gtatcaaatg cgtatttatc ttcacgacta acgtccaacc
     5641 ccaggatatt aacattcccg ttgctgaggt cacttagctc tctctttaat tctggaacaa
     5701 ctttttcttt taaaatttta agttgttctt cggaaaatga ttcgctttta gtatagtcgc
     5761 cagtgcctct tattctgtta tataaatcgt taatctgctc ttggctaagc gaagcttcta
     5821 atcgccttgc cagttggtcg aatatataag ctcgtaattt ttgagctttc tgagtctttc
     5881 cacgttcaac gacctctttc aaaagatcat tggttagttg atttaaagcc ttattaacag
     5941 gagagcgtcc aactgtcata gctccatata cttgagcctt gttctcaaca ggctttttcg
     6001 catccttagc aggctgattt tcgactgcat cagaaggaaa cggagtatcc aagtccttat
     6061 tttttcgaac ccaatctaag gcaacctctt tcccaatttt aagataaagg gcaaatttag
     6121 ttaaaaacac ctcattaatc agtgtttgtt cattaactcc agtgtttaac tggtgaattt
     6181 caagcaatga atcagccaat tctttagcca tttgctcgtc aacagcgcat gtcgatttga
     6241 tattctcaat aaaaattgcc ttttgttgct ctttcatata tttatcccac ctggttataa
     6301 tctaaatttt tcctgttgtc tcaactggag aaaacaccaa aaataaacta aatgtttaca
     6361 aagtggtcat gaatatatca taaataactt aa
//
LOCUS       DATNSN010000166         6274 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.166, whole genome shotgun sequence.
ACCESSION   DATNSN010000166 DATNSN010000000
VERSION     DATNSN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.166"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            121..540
                     /locus_tag="VAH76_002662"
     CDS             121..540
                     /locus_tag="VAH76_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEO1387252.1"
                     /translation="MNNYRQQHGLAPLKMDNRIVKEAKIHSMDMAKHAIPFGHKYFKK
                     RIDKLHTQIKNSNAGAENVAYNYKNAQDVVKNWLRSPGHKRNIVGNYDLTGVGIARDE
                     KGKIYFTQIFLKTGNSHYASRKPFPSIFSGALFSKKA"
     gene            934..2067
                     /locus_tag="VAH76_002663"
     CDS             934..2067
                     /locus_tag="VAH76_002663"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEO1387253.1"
                     /translation="MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDF
                     ANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPL
                     VKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
                     AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQE
                     QDSETAGGYATETSEEYKKLQAELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQM
                     KPGSVIVDLATSRGGNCELSVADKTIKHGEITIVGLSNMAGLVPATASDLYSNNLVHL
                     INLLAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA"
     gene            2060..2356
                     /locus_tag="VAH76_002664"
     CDS             2060..2356
                     /locus_tag="VAH76_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEO1387254.1"
                     /translation="MPEIHTLDNPYITILTIFVLACFVGYYVVWKVTPALHTPLMSVT
                     NAISSIIILGALIAAGSELIGCITWLGGIAIFITSINIFGGFVVTQRMLRMYKK"
     gene            2380..3777
                     /locus_tag="VAH76_002665"
     CDS             2380..3777
                     /locus_tag="VAH76_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEO1387255.1"
                     /translation="MNSIVPLFYLLAAICFILALKGLASPATSRRGNLLGILGMILAV
                     GSTLMMPTTYHHILLIALIAGGGIIGTIIALKINMTAIPQLVAAFHSLVGMAAVLVAY
                     CAFLSPASFQIGTPGAIATASLIEMSLGLIIGAITFSGSVIAFLKLQGLISGIPIRFY
                     AQNYVNLLIGLTILVLLILFVANQNLFLFNLLAGLAFLIGVLLIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFTLSNHLLVITGALVGASGAILSYIMCVGMNRSIFNVIFGGINSS
                     GNTKQAHSANELRNVRQANGEDAAFLLSNAKDVIIVPGYGMAVAHAQHAVKELVDALE
                     SRSIRVRFAIHPVAGRMPGHMNVLLAEANIPYDRVFEQSEINRDFATCDVAYVIGAND
                     ITNPAAKTDPSSPIYGMPVLEVEKAKTVLFVKRSLSPGYAGVENDLFFHDNTYMLFGD
                     AKVMTESIAKALGEI"
     gene            3912..4295
                     /locus_tag="VAH76_002666"
     CDS             3912..4295
                     /locus_tag="VAH76_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387256.1"
                     /translation="MLRKIGFTLLCAVATFSSSTYSSVTHTLQAGVTLEYEFPPNEPL
                     VFVNYLYWEVAADCKITSEDPSNELFAEALAKAGKINGVTLSKGNTMKVTVHSGDILK
                     LSAESGAKVQITNFGPHTLKATCTA"
     gene            complement(4361..5941)
                     /locus_tag="VAH76_002667"
     CDS             complement(4361..5941)
                     /locus_tag="VAH76_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO1387257.1"
                     /translation="MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTV
                     KGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
                     GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIG
                     DSADELREEIELVKGASHGFNLEDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGP
                     QPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSGAYKKGMKLSHLRIG
                     KEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAP
                     ELFRLVRLRDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRL
                     KHEYKVDCIYESVNVTCARWVYSEDDKAMSEFRTKAYDYLALDGGDMLMYLAPTKVNL
                     TMAEERYPKIKFCATREH"
BASE COUNT         1937 a         1306 c         1183 g         1848 t
ORIGIN      
        1 attaaatcta aaaccctatt catggtgctt gtatacagtt tgctctcccc tgcttatgca
       61 gcatcacacc ccaaaagcac agtgaatgac accgccattc aaaatgaaat attagtttat
      121 atcaataatt accgacaaca gcatggcctg gctcctctca aaatggataa ccgcatagta
      181 aaagaagcaa aaattcattc catggatatg gcaaaacacg caataccatt cggccataaa
      241 tattttaaaa aacgtattga taaattacac acacaaatta aaaactcaaa tgcaggagct
      301 gaaaacgtgg cttacaacta taaaaatgcc caggatgttg tcaaaaactg gttacgtagc
      361 ccaggacaca aaagaaatat cgttgggaac tatgacctta ccggggtagg tatagctcga
      421 gatgaaaagg gaaaaatata ttttactcaa atattcttaa aaacaggaaa tagccactat
      481 gcctccagaa aacccttccc atcaattttt agtggtgccc tcttcagcaa gaaagcataa
      541 aaattaaaat agggtgttta acaaaatttt tgcaattttt aagtacaatt gagtggaatt
      601 aatgacaacg ttagcctaat attgaaacat agacggcaat cattgttcag ccaacagtac
      661 aattttggag gccagattga gagtgtggtg agcaagctca gcctagactg agaagccaaa
      721 agcactccgg aggaatccac agttttaaac aggactgcac tcaatcttgc tgtctataac
      781 atactaacac catggggcgt attcaatacg ccctttttat tggttcattc ctcgcttgtc
      841 cggatccaaa gatccaaaat tggtatttta tgctgtagaa aattattcta aattacaata
      901 ggatagaata ataacggtaa taaggatttt cccatgatga ttgcaacctt aatggaatca
      961 gctcaggaaa cgcgagtagc tattactcct aactcggcca aacaatacat aaaactaggc
     1021 tatgatgtag ggattgaaaa aaacgcgggg cttagctcag attttgctaa cgaagaattt
     1081 gaaaaagctg gcgcaataat attcgatagt aaaagtacat tactcaaaaa aacacaaatt
     1141 cttttatgtg ttaacgaacc tgatcccaaa aacctgaatg gcttacctcc agattctcta
     1201 ataattggac atatagacaa taacccactt agcccgcttg ttaagtggtg tctggatcaa
     1261 aaaatgacat tattttcaat gaatctcatc cccaggataa gtcgtgctca aagcatggac
     1321 agcctatcgt ctcaggctaa cctggctggt tacagggcag ttttggaagc tgcggcaaca
     1381 tttcatagag ctatacctat gatgatgaca gcagcaggca tgatacaacc ggccaaagca
     1441 cttattcttg gtgccggagt tgcaggcttg caagccatag ctactgcgaa acgtttaggt
     1501 gcagtggttt atgcctttga cgtaagaaag gcagccaaag aacaggttga aagtttaggc
     1561 gctgaattca tagaggtaat gcaggagcaa gatagtgaaa cagctggggg atatgccaca
     1621 gaaacaagcg aagaatataa aaaacttcag gctgaactca ttgatcaata tgcaaaaatt
     1681 gcagacattg taatttctac tgccttgata ccagggcgtc aagctccggt tttactttat
     1741 aaaaaaactg tcgagcaaat gaaaccaggt tctgtcattg ttgatctcgc aacgtctcga
     1801 ggtggaaatt gtgaactcag tgttgccgat aaaaccatca agcatggtga gattactata
     1861 gtgggattaa gtaatatggc aggtctcgtc cctgctacgg caagtgacct ttattctaat
     1921 aatctggttc atcttataaa ccttttggcg aaaaatccgg ctgaacttaa cttaaatcct
     1981 gatgatgaga ttatccagca agccgttctt tgccataaag gaacttacct gccctttcaa
     2041 ggagtaaagg agaagcaaaa tgcctgaaat tcatacactt gataatcctt atattacaat
     2101 attaaccatt ttcgtactgg cctgttttgt aggttattat gtggtttgga aagtaacacc
     2161 ggctttacat acacccctaa tgtcagtaac caatgccata tccagtatta ttatacttgg
     2221 tgctttaatt gctgcaggaa gtgaattgat cggatgcata acctggttag gtggcatagc
     2281 catattcatt acttcaatta atatttttgg tggctttgta gtaactcaac gcatgcttcg
     2341 catgtataaa aaataatgat ttaaaggacg gaatcagtaa tgaattcaat agttccactg
     2401 ttttatttat tggcagccat ttgctttata ttggctttaa aaggactggc cagtccagct
     2461 acttcaagaa gggggaacct tctaggcatt ctgggtatga tcctggcagt gggctccacc
     2521 ctgatgatgc ccactaccta ccatcatatt cttttaatcg ccttaatagc aggcggcgga
     2581 ataataggaa ctattattgc tttgaagatc aacatgacgg ccataccaca actggttgct
     2641 gcttttcatt ctttagtagg catggcagca gttctggtag cctattgtgc ctttttatcc
     2701 cccgcgtcct ttcaaatcgg cacacctggt gctattgcta ccgcaagtct aattgaaatg
     2761 agcctgggat taatcatcgg agccattacc ttttctggtt cagtcattgc ctttttaaaa
     2821 ttgcaaggac tcatatcagg cattccaatt cgattttatg ctcaaaacta tgtcaactta
     2881 ctaataggac taacaatcct ggtgttactc attttatttg tagccaatca aaacctgttt
     2941 ctctttaacc tgctcgcggg attagctttt ctcattgggg ttttactgat tattcctatt
     3001 ggcggagccg atatgcccgt cgttatttcc atgctgaact cctactcagg ttgggccgca
     3061 gcaggaatag gtttcactct gagtaatcat ctgttagtta tcacaggcgc tctggtaggc
     3121 gccagcgggg caattttaag ctatatcatg tgcgttggta tgaatcgctc tatttttaat
     3181 gtcatttttg gtggaataaa cagttctgga aatacgaaac aagctcattc tgctaatgag
     3241 ttacgcaatg tcagacaagc caacggcgaa gatgctgcat tcctattaag taatgccaag
     3301 gatgtcatca ttgttcctgg atatggaatg gcagtggcac atgctcaaca tgcggtgaaa
     3361 gagcttgttg atgccttgga atctcgatct attcgtgttc gttttgcaat acaccctgtt
     3421 gccggacgca tgcctggcca tatgaacgta ttacttgctg aagcaaatat cccctatgac
     3481 agagtctttg agcaaagtga aattaacagg gattttgcta cctgtgacgt tgcttacgtg
     3541 attggagcta acgatatcac caaccctgca gctaaaactg atccttcctc ccccatttat
     3601 ggtatgcctg tcctggaggt tgaaaaagct aaaacggtct tattcgtcaa acgcagttta
     3661 tctcctggtt atgccggtgt ggaaaacgat ttattttttc acgacaacac ctacatgctt
     3721 ttcggtgatg ccaaagtaat gactgaatca atagctaagg ctttgggaga aatataaccc
     3781 tttcaaaatt atagcaataa tctctgacgg ggtgggtacc cggttcaatc gacgcaaatg
     3841 ccttttaatt tgccaacgaa gcaaaataaa tgtatcttag ccagttaatt tttcataatg
     3901 gagatatatc tatgctacga aaaatcggct ttacgttgtt atgcgcagtg gcaacattta
     3961 gttcaagcac ttattccagt gtgactcaca cactccaagc tggagtaacg ctggaatacg
     4021 agtttcctcc taatgaacct ctagtctttg ttaattattt gtattgggaa gtagcggccg
     4081 attgtaaaat taccagtgaa gatccatcca atgagctttt tgcagaggca ctcgcaaaag
     4141 ccggtaaaat taatggtgtt acgctctcta aaggaaatac catgaaagtg actgtccatt
     4201 ccggcgatat tttaaaacta agcgcggaat ctggagcaaa agttcaaatc accaattttg
     4261 gaccacatac tctcaaagca acttgtactg cataaagaat tgagctaacc gcaccccaac
     4321 actgaacagt aatggggtgc gaacattcaa atcctgttta tcaatgttct cgagtagcac
     4381 agaacttaat cttgggataa cgttcttctg ccattgtcaa attcactttg gtaggcgcca
     4441 ggtacataag catatctcct ccatccaaag ccaaataatc ataagccttg gtacgaaatt
     4501 cactcattgc tttatcatct tcagaataaa cccatcgagc gcaagtcaca ttgactgatt
     4561 cataaataca atccacttta tattcatgtt tcagtctatg cgctaccaca tcaaattgta
     4621 atatcccaac agctcccaat attaactgat tgctgtttaa tggcctaaaa acctgggtgg
     4681 cgccctcttc agataattcg attaatcctt taagcaaggc tttactcttt aatgggtccc
     4741 tcaacctgac aagcctaaac aattcaggag caaaattagg aattccagta aactttaatt
     4801 gctcaccttg tgtaaaagta tcaccaatac gaatagtacc atgattatgc aaaccaataa
     4861 tatcaccagc taaagcaagt tctgtgtgtg atctatcgcc tgccataaaa gttaaggcat
     4921 tggaaatttg cacctctttt cctatgcgta aatgacttaa tttcattccc ttcttgtaag
     4981 cgccagaaca aaccctcaga aaggcaattc ggtctctgtg tttgggatcc atattggctt
     5041 gaattttaaa aacaaaaccg gaaaatgttt cttcattcgg agaaacaact ctctcctgag
     5101 ttgctcgagg ttgaggtcct ggcgcatact cgacaaaatc gtccaataat tctttaatac
     5161 caaaattatt aattgctgaa ccaaagtata ccggggtcat ttttcctgca agataatctt
     5221 ccaagttaaa cccgtgggac gctcctttga ctaattcaat ttcttctctg agttcgtctg
     5281 cactgtcacc tatcaactca tctaattgag gattatccaa tcctttaatc tgcatagctt
     5341 ccactttttt tgcattactg ccttgctgat ataaataaat aatatcttga tagcggtgat
     5401 aaattccctt gaagcgttta cccattccaa ctggccaggt aattggcgca cattgaatgc
     5461 ccaaaacaga ctctacttca tctaacaaat caataggttc tcgcccttct ctgtccaatt
     5521 tattgataaa agtcatgatc ggtgtatcac gtaaacggca aacttccatc aattttaccg
     5581 ttcgctcctc tactcccttg gccacgtcaa ttaccattaa ggcagaatct acagcagtga
     5641 gagtacgata ggtatcttca gaaaagtctt catggcctgg tgtatctaac aaattgatga
     5701 cgtgttgatt atgtataaac tgcataacag acgtggtaat cgatattcct ctctcttttt
     5761 ccatttccat ccaatcagaa gtggcatgtc tgtctgcttt acgacctttg accgtacctg
     5821 ccaactgaat agctcctcca aaaagcaata acttttcagt tacagtggtc tttcctgcat
     5881 cagggtgaga aataatggca aaagttcgcc ttttattaaa gtcttgataa aaatcagaca
     5941 taggaatctc ggattaagtg gttaaatgcc ttgaattgta aaggaaaaga acaaaactaa
     6001 caattttttc gttataaatt aaaatctcgc tggtgtttat ccatttcatt atagtgttca
     6061 ggagtgcaac tcaaatttta aacttaaaaa tcaattattg atcaatttga tttttattga
     6121 taaaattgcc gtttttttgt ggagccgtac atgtcaacgt cagaatcaga attaagaaaa
     6181 tcaatgcaac aatatggaaa acaggcattc agtgaaacac aacaaataga gattttgaat
     6241 ttgttacttg aagatgccaa aaaacagctt ccca
//
LOCUS       DATNSN010000167         6261 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.167, whole genome shotgun sequence.
ACCESSION   DATNSN010000167 DATNSN010000000
VERSION     DATNSN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.167"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..413
                     /locus_tag="VAH76_002668"
     CDS             <1..413
                     /locus_tag="VAH76_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1689 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387258.1"
                     /translation="AKTLLICIKQPERTSYAIHNYHKGFYYPVAIHDKIKPDPTIENA
                     AIATLGVSLALLLGSIPTFIFNPLFGVIMVSLAVTLLLPSGFYLLIPDSPDTTSKKEE
                     EKRIFMEGAKIINPDVRIEEFDEQPYLSSSLIKT"
     gene            498..3173
                     /gene="acnA"
                     /locus_tag="VAH76_002669"
     CDS             498..3173
                     /gene="acnA"
                     /locus_tag="VAH76_002669"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEO1387259.1"
                     /translation="MKVGQDSLSTKSQLTVDGKTYNYYSLKEAENKHFKGINRLPYSL
                     KVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDL
                     AAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYE
                     FLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENDGQLYAYPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQM
                     LRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGR
                     DKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSL
                     PVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLV
                     AKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNS
                     GPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCS
                     DLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAKVSGTMFRKEYAEVFKGDAHWQAIQ
                     TSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGS
                     IKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTR
                     YVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESF
                     ERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDG
                     DIEKIETLCRIDTADELEYYKNGGILQYVLRKISS"
     gene            3438..4832
                     /locus_tag="VAH76_002670"
     CDS             3438..4832
                     /locus_tag="VAH76_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1387260.1"
                     /translation="MNHSKKVLNVFSLVMINVIAVDSLRTLPISAKLGFSLVFYYIFA
                     ALTFFIPVALVAAELATAYPNTGGIYVWVREAFGRRAGFITIWLQWIYNVVWYPTMLA
                     FIAATLSYLIAPHLGNNKFYLLGTALTLFWVFTFLNCFGMKLSSIVSIIGASIGTLLP
                     MIVIIVLGAVWIFQDRPVAVNYPTTWLPDFSSLGNLSLFSAVLFGLIGMEMSAVHAEE
                     VKNPQRDYPKALFYSALLIISTLSLGSLAIVIVVPNDSLSVVSGLVDAYAIFFNSYNM
                     PWMTSVIAVLIILGGLSGVSAWIIGPTKGLLVSARDGSLPALFSRVNKYGSPVAILLT
                     QGVIFTVLSTVFILLDSINAAYWVLSDLSAQMALLVYIMMFAAAIKLRYSKPEQPRGY
                     TIPGGNLVMSLISGIGIICCIAAMIVGFIPPSQIPIKNVFLFECFLIGGLILFVFIPW
                     LFAKKHDEQLCSEE"
     gene            5126..>6261
                     /locus_tag="VAH76_002671"
     CDS             5126..>6261
                     /locus_tag="VAH76_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1692 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387261.1"
                     /translation="MARSKEEKALEPKDAENKFRVLTSDVAGNIPTHSSVTDVLNLIY
                     SSITTHNLFKPHLEPLKAKACVVQGDLATLILIAQRNPAALFQKGNITDPRERTFYNV
                     SAWQLILFLCDVDMKNQLMPFIPEQFKAIAQKQTNELGSGGADLIKLSRDPQAVAFED
                     FDGLTQFKQTLPLIDGTRKEVTFPLLENVDGIVYFQDKNKEVHFYYANRESKTITSLV
                     PDLKSEEDKQLFAAFKASFDAMENNSSRRSSDTEHQLIAKLFNAKPQRQGIQYEHQGI
                     RYQDSRTSFSLINAYRTSIRLYDEVQNYQYEKADTYWREVVGKAQGEEMWLLERICEE
                     GRPFHPLPADFKEFKRGFTFYNWITNKVESAFAGGKLAVGLGSD"
BASE COUNT         1901 a         1233 c         1251 g         1876 t
ORIGIN      
        1 ttgcgaaaac cttgctcatt tgcataaagc aacctgaacg aacatcctat gcaatacaca
       61 attaccataa aggattttat tatcccgttg ctattcacga taaaattaaa cccgatccca
      121 ctatagaaaa tgcagctata gctactcttg gagtaagcct tgctttgctt ttgggttcaa
      181 taccaacatt catttttaac ccactctttg gtgttatcat ggtatcacta gcggtgacac
      241 tgctgttacc aagtggattt tatttactaa tcccagattc tccagataca acaagcaaaa
      301 aagaagaaga aaaaaggatt tttatggaag gtgcaaaaat cattaatcct gatgttagaa
      361 tcgaagaatt tgatgaacaa ccttacctct cctcttcatt aatcaaaaca tgattttgtt
      421 gatactgaat gaaaacttcc tgaaattgag ctattattcc tagagattat taataataat
      481 gattttggga gtactttatg aaagtgggtc aagacagtct atcaacaaaa tcacaattaa
      541 cagttgatgg aaaaacctat aattactata gtttgaaaga agctgaaaat aaacatttta
      601 aaggaattaa tcgcctccct tactccttga aagtacttct cgagaattta ttacgttttg
      661 aggatggcaa tacagtaacg accaaagaca tcaaggcaat tgccgactgg ctccataata
      721 aaacatccca gcatgaaata gcatttagac caactagagt cctgatgcaa gacttcactg
      781 gtgttcccgc agtagtagat ttggctgcca tgcgtactgc catagtaaaa atgggaggta
      841 acgcagacaa aatttctcct ctttctcctg tcgatttggt catagaccat tcagtcatgg
      901 tagataaatt tgcttcagct gatgcccttg aagtgaacac caaaatcgag atagaaagaa
      961 ataaagaacg ttatgagttt ttacgctggg gacaaaaagc tttttctaac tttcaggtcg
     1021 tcccacccgg aacagggatt tgccatcagg tcaacctgga gtatttagga aaaactgtgt
     1081 ggaatagtga aaatgatggc cagttgtatg cctatccgga cactttggtc ggcacagatt
     1141 ctcacacgac catgattaat ggactaggtg tcctgggttg gggagtcggt ggtatagagg
     1201 cagaagcagc catgctgggg caacctgtat ccatgctaat ccccgaagta atcggtttta
     1261 aattatcagg taaacttaaa gaaggcatta ccgcaactga tttggtcctt accgtcactc
     1321 aaatgctgcg aaaaaaaggg gttgttggta aatttgttga attttatggt cctggactta
     1381 atgatttacc tttggcagac agagcgacaa tttccaatat ggctcccgag tatggcgcaa
     1441 catgtggctt ctttccggta gacaaggaaa caataaagta cttggaatta actggtcgtg
     1501 acaagcatac cattgccctt gttgaggctt atgcaaaagc acaaggtatg tggtatgaca
     1561 aagacaatga agaacctgtg tttactgact ctttacatct ggatttaggc agcgttgaac
     1621 cctctctggc tggcccaaaa cgcccccaag ataaagtcaa tctcagttca ttacctgtag
     1681 aattcaacaa ttttttaatt gaagttggta aagaaaaaga gaaagaaaaa acctttgctg
     1741 ttaaaaacaa agattttcaa atgaaacatg gtcatgtagt cattgcagcg ataaccagct
     1801 gtactaacac ctctaatcca agtgtactaa tggcagcagg actggtagca aaaaaagcga
     1861 ttgagaaagg attacagcgc aaaccctggg ttaagtcttc attggcacct ggttctaaag
     1921 tagttactga ctatttaagg catgccggcc tacaaaccta cctggatcaa ctgggtttta
     1981 atttggttgg ttacggctgt accacttgta taggtaattc gggtcctttg cctgatgata
     2041 tttcccattg tgttgcagag catgatcttg ttgtttcatc tgtactgtct ggtaatagaa
     2101 atttcgaagg gcgtgttcac ccgcaagtca gagccaactg gctggcttct cccccattag
     2161 tagtcgctta tgctctatgc ggtacaactt gcagcgattt aagcagggaa cccattgggc
     2221 aagacaaaga aggaaacgat gtttatctca aggatatctg gccatctaat gaagagattg
     2281 ctgcggaagt cgctaaagta agcggtacca tgttccgtaa agagtatgct gaagtattta
     2341 aaggagatgc tcattggcaa gccattcaaa caagctcagg gcaaacttat gaatggaatc
     2401 ctgattctac atacatacaa caccccccat tttttgaaaa tttaagtctg aaaccagaac
     2461 cgctcaaacc tatcaagcag gcttatgttt tagccctatt cggcgattca attacaacgg
     2521 atcatatttc acctgcaggt tcaataaaag ccagctctcc agcaggctta tatttaaaat
     2581 ccaaaggggt agatgagaaa gattttaact cctatggatc acgccgcggt aaccacgagg
     2641 tcatgatgcg aggcactttt gccaatatac gcattcgtaa tgaaatgact cctggacaag
     2701 aaggtggcgt cactcgttat gttcctactg gagaaaccat gtcgatttat gatgcagcca
     2761 tgcgttatca ggaaaatcaa caagacttgg ttatcattgc tggtaaagaa tatggaacag
     2821 gctcttctcg cgattgggca gcaaaaggca ctaatttgct tggtgttaaa gctgtgatta
     2881 ctgaaagctt tgagcgaatc catcgttcca atttaattgg tatgggtata ttgccattgc
     2941 aatttaaaga gggtactact cgtaaaacat taaaattgga tggtagcgaa cgcatcagta
     3001 ttgaaatctc tgataaatta accccgggcg cgatggtacc tgtaaccata gaaaggcaag
     3061 atggggacat tgagaagata gaaactcttt gtagaattga tactgctgac gagctcgaat
     3121 actataaaaa tggcggaata cttcagtatg tattgcgcaa aataagctct taacaattgg
     3181 gcacattgcg tgcccttctc ccttagagtg ttgggttcac aaaatatcta gagacgcatg
     3241 aacactgcat cactaggcag ctgaaatcag aatatcagta aaaattgata ccataatcag
     3301 gagaaaccca acaacttata aatattaagc agtaattaaa ctgtacagac aaacttttct
     3361 ttactctgtt aacttatcat aataccattt ataccccatt caatacccgg gtttattgca
     3421 ctcataaagg ataaataatg aatcattcaa aaaaggttct gaacgttttt tctttagtta
     3481 tgatcaacgt cattgctgta gatagtttga ggaccttgcc tatcagcgca aaacttggat
     3541 tttctctggt cttttattat atttttgcag cccttacctt ttttattcca gtagcattgg
     3601 ttgctgcaga gctcgctacc gcctatccta ataccggcgg aatttatgtc tgggtcaggg
     3661 aagcattcgg gagacgggca ggatttatca ctatttggct gcaatggatc tataacgtag
     3721 tttggtatcc aactatgtta gcttttatcg ccgccacgtt atcctattta atcgctcctc
     3781 atttggggaa taacaaattt tatttattag gcacagcact taccttgttt tgggtgttta
     3841 cttttttgaa ttgctttggc atgaaactat ccagcattgt aagtattatc ggcgcctcca
     3901 taggcacact acttcctatg atagtgatta ttgtcctggg cgcagtttgg atatttcaag
     3961 atagacctgt agcagtcaat taccccacaa catggttacc tgattttagt tctttaggaa
     4021 atctgtcttt attttcagcg gttttatttg gccttatagg aatggaaatg tctgctgttc
     4081 atgctgaaga agtaaaaaac ccgcaaagag attatccaaa agcacttttt tattcggctc
     4141 ttttaattat atccacctta tcattaggtt ctttggcaat tgttattgta gtaccaaacg
     4201 atagtttaag tgttgtgtca ggccttgttg atgcctacgc catatttttt aattcctata
     4261 atatgccgtg gatgacatca gtgattgccg tgttaattat tttaggaggc ctcagtggcg
     4321 tttcagcctg gataataggt ccaaccaaag gattactagt gtctgcacgc gatggttcct
     4381 tacctgcact gttttcccgt gtcaataaat acggctcgcc ggtagccata ctgctcactc
     4441 aaggcgttat atttactgta ttaagtacag tctttatttt actcgattca attaatgctg
     4501 cgtactgggt attaagcgat ttaagtgctc aaatggcatt gttggtatat atcatgatgt
     4561 tcgctgcagc aattaaattg cgatacagca agcccgaaca gccgcgtggc tatactattc
     4621 ctggtggaaa tctggtaatg tctcttatct ctggaattgg gatcatctgc tgcatagctg
     4681 ctatgattgt cggatttatc cccccttcac aaatccctat aaagaatgtt tttctctttg
     4741 aatgttttct gatcggcggc cttattctat ttgttttcat tccctggtta tttgctaaaa
     4801 aacacgacga gcaattatgt agtgaagaat aattgagcaa gaaatgtaag cccacaaaaa
     4861 attttgttgg gcttttcaag atgattctta aaagttgtta atcaattgta atatacccaa
     4921 taaacttcaa actgcgagtt ttacagcctc ttctttcgca atattctcac gcataatatg
     4981 cttttttaaa tcaccaaggc tgcttagtat tgttttctac ctttcgataa ttgccataat
     5041 atttgccaca ttaaattgtt tttatatgaa cattaaattt aatgtataat gctcaatcat
     5101 attgtgaaaa taaccgaatg tatgtatggc acgcagcaaa gaagagaagg cattagaacc
     5161 aaaagatgca gaaaataagt ttagagtctt aacaagcgat gttgctggaa atatacccac
     5221 tcattcctct gtaacggacg tattaaattt aatctattct tccattacca cccataactt
     5281 attcaaaccc catttagaac cattaaaagc caaggcttgt gtcgtgcaag gcgatttagc
     5341 tacattaata ttgatagcac aacgcaaccc tgccgcgtta ttccaaaagg gaaatatcac
     5401 cgacccaaga gagcgaactt tttataatgt atctgcctgg caacttatcc tgtttctgtg
     5461 tgatgtggac atgaagaatc aactgatgcc tttcattcca gagcagttta aggccatagc
     5521 tcaaaagcaa accaacgagc ttggcagtgg cggcgctgat ttaattaaat tatcccgtga
     5581 cccacaagcc gtggcttttg aagatttcga tgggttaact caatttaaac aaactctccc
     5641 cctcattgat gggacacgaa aggaagtcac tttcccattg cttgaaaatg ttgatgggat
     5701 tgtttatttc caagacaaga ataaagaagt tcatttctac tatgcgaatc gagagtccaa
     5761 aacgattaca tctctcgtac cagacctcaa gtcagaagaa gataaacaat tatttgctgc
     5821 atttaaggcc tcctttgatg ccatggaaaa caattccagt cgtcgctcaa gtgatactga
     5881 gcatcaattg attgcaaagc tatttaatgc caaacctcag cgtcaaggca ttcagtatga
     5941 gcaccaagga atacgttacc aggatagtcg cacttcattt agtttgatta atgcctatag
     6001 aacaagtatt agactgtatg acgaagtaca aaattatcaa tatgaaaaag ccgacaccta
     6061 ttggcgcgaa gtggtaggta aagcacaagg tgaagagatg tggcttttag agcgaatttg
     6121 tgaagaaggc cgtccattcc accctttacc cgcagatttt aaagagttta aacggggatt
     6181 taccttttat aattggatta ccaacaaggt agaatctgct ttcgcggggg ggaaacttgc
     6241 agtcggtcta ggttctgatt t
//
LOCUS       DATNSN010000168         6234 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.168, whole genome shotgun sequence.
ACCESSION   DATNSN010000168 DATNSN010000000
VERSION     DATNSN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.168"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1166)
                     /locus_tag="VAH76_002672"
     CDS             complement(<1..1166)
                     /locus_tag="VAH76_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO1387262.1"
                     /translation="MKFTDTYIRNLKPQQKWFEQIEFSGLGIRVMPGGGKSWIFRFTF
                     DGKRYKMTLGKYPGIGLKEARELMLDAEHLKEQGINPIEYAKQQQAKSDNTVKKLAFS
                     WYTQYVEKHLKRPLTVKKQIDGDIINLLGDRVLDELETKDITEALDKIVKRGASVHAN
                     RVLSSLKQMFGYAVSRGTMSTNPASNIRARDIGGHEKPRERVLSLDEIKSLWLFLDSK
                     DSQMAPQTRIAIKIILLTGVRTAELRLAQWSEINFDDSLWTIPATHSKASIVHKVHLS
                     DLTKQLLQQLKSISRSDYVLTGADYRQPLTENALPRAIKRIQERLGIPEWTAHDLRRT
                     FATQLGETLQVDPVVIEKCLGHKMPRIMATYNRNEMLPQRREALERWAVCIENM"
     gene            complement(1422..1769)
                     /locus_tag="VAH76_002673"
                     /pseudo
     CDS             complement(1422..1769)
                     /locus_tag="VAH76_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1869..2816)
                     /locus_tag="VAH76_002674"
     CDS             complement(1869..2816)
                     /locus_tag="VAH76_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEO1387263.1"
                     /translation="MANLFSFLFLGTLGWGISLYLMKILLVSLTPTEIVLYRMAIGAA
                     SLFILAKLFKLKTDNIRGLILDGIIVGTFNMTLPFYLTTYAVKTVSSSLASVINGLTP
                     LCTLILGMVFFSSKQKLSVLNLLSIVLGFIGIIIINSDVSIYQGSAVEFIALLATTIS
                     YGVTANYFKYHARTKDPILVSAVSAFVSAITMLIFKCATESSINYWGIPQDMHQIIAL
                     LWLGVIGSGFCLYLYCSLIQNAGAVFASMITYLMTITGVIMGMIFLHETISHQVMIGC
                     IFIFFSLILVNHAPLLEKILVSISRQISLKSARRYELNQ"
     gene            complement(2800..3390)
                     /locus_tag="VAH76_002675"
     CDS             complement(2800..3390)
                     /locus_tag="VAH76_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1387264.1"
                     /translation="MSQSNVIIVHGAYGHPFENWFNWMKTELESLGIECFVPQLPTPD
                     GQELKNWLHVFNSTVSPMITHDTILIGHSLGSAFLLRWLEQANQSVFSTILAGPFIGA
                     VGIQKFDKINESFFENGFDWHSIINKSKQFFCYHGSNDPYISRSNFNFIANNLQARKI
                     IISQGGHLNEAAGFSSFPQLLIQLKLLLGINYGKFI"
     gene            complement(3411..4715)
                     /locus_tag="VAH76_002676"
     CDS             complement(3411..4715)
                     /locus_tag="VAH76_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEO1387265.1"
                     /translation="MSIHLINYTTQPEFIKLIVQEYKNFDLTKKTILFIEPYGAVLSL
                     LKRGLEKKYNIIVLTANSDFRVVPEEFLNQAALNINIDTANNNSVLELAGTLQQHMSI
                     DAAIPGFEYFVPIASRVSALLKVPGIDCKHVLNLRRKDLMRTALNKAGLNSPRYYLVT
                     SLDDINEAVDYIGFPAICKPIDAAGSVHVKKVKNKKELLEAANRILNGNDVLWGHKLS
                     SSLLLEEYIDGKEYSLEGTVQDGVIAHFSTTEKFVSDQSEFIEIGHIVNAPIEQQLHT
                     KIQNYIDQVIKILGADNCPFHAEVRLRRDGEPVLMEIAARLAGDKIGDLLCLSRGINY
                     FDYVYAAYLGIKMPLVEMKNNYAGIRFFYRPHTDMFAIVTGMDAAKMQAIEDIAIYYN
                     AHVAIPEFPKPLRRLGHVIAKSDDYNKLSKMLHDIDSDIHFN"
     gene            complement(4732..5553)
                     /locus_tag="VAH76_002677"
     CDS             complement(4732..5553)
                     /locus_tag="VAH76_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1387266.1"
                     /translation="MMCYMLVSNEKVIAEEWEKYAGYTVRPLPSTCAYYKDLIQSNSN
                     KDSFLIYGGTPEIRSLFQESNLKVTLTDQSELIVRAMGYLTMGAAPIAANEELVVTNW
                     LNLEGIANDSCDFLIGDDAINMVEWTQFDLFLSNANRVLKSNGLFICHLLVKPQECFI
                     TNTIDDVRREYKEGVIRTKYDLASRLNFICYDTNSYAMGWQNTIEQLGINQLDQFKPS
                     FDFVGTFGRCNSKFYCPPQDAFERLLTNYFHIEEVFYPHEYEYCQFEPVYVLRKK"
     gene            complement(5559..>6234)
                     /locus_tag="VAH76_002678"
     CDS             complement(5559..>6234)
                     /locus_tag="VAH76_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEO1387267.1"
                     /translation="LVRDVLQKHEVFNHPIFDYLMHDASIEDLKKFAYSESIMNLEFF
                     DYLALAVIGVSDQAKVEIMANLWDEAGQGCAEKFHTVLFQNFLNDLGITYDRKQILEG
                     LSWEGVAGINLFNYFSIYPFNKTKYFGMLAATEMLDPPHYNKLIKGIMRIFSSHNIDY
                     SYYTEHELVDAHHASGWLNHVVIPELARKPYKTQDFWLGFYMRLDSTKKYYDQLLNSL
                     IMKKAA"
BASE COUNT         1975 a         1250 c         1048 g         1961 t
ORIGIN      
        1 atcatatttt caatacatac cgcccaacgc tcgagagctt ctcgtctctg aggtaacatt
       61 tcattgcggt tataagttgc cataatccgt ggcattttat ggccaaggca tttctcaatt
      121 accactggat caacttggag cgtctctcct agttgggtgg caaaagtacg gcgtagatca
      181 tgggctgtcc attctggaat acctaaacgc tcttgaatgc gtttaatagc ccttggcaaa
      241 gcattttctg tcaatggttg gcgataatct gcacctgtta agacataatc tgatcgacta
      301 atggatttga gttgctgcaa taactgtttg gtcaaatcgc taagatgtac tttatgaaca
      361 atactagcct tggaatgcgt agccggtatt gtccaaaggg agtcatcaaa gttgatttcg
      421 ctccattgcg ccaagcgcag ttcagcagta cgaacaccag ttagtaaaat aatcttaata
      481 gcaatacggg tttgtggtgc catttgagag tctttactgt ccagaaataa ccacagggat
      541 ttgatttcat ccagacttaa aacacgctct cttggtttct catgaccgcc aatatctctt
      601 gcgcgaatat tagaggctgg attagtgctc atagtgccac ggcttaccgc atagccaaac
      661 atttgcttca aagagcttaa aacgcggttt gcgtgtacgc ttgcccctcg tttgactatt
      721 ttatccaagg cttctgtaat gtctttggtt tccagttcat ctaatacccg atcaccaagc
      781 agattaatga tgtcaccatc aatttgcttt ttaaccgtta agggacgctt taggtgcttt
      841 tcaacatatt gggtatacca gctaaatgcc agctttttta ccgtattatc tgatttggct
      901 tgttgctgtt tagcatactc tatgggatta atcccttgtt cttttaaatg ttccgcatcc
      961 agcatcagct cacgtgcttc cttcaagcca atccccgggt acttacccaa agtcatcttg
     1021 taacgcttgc cgtcaaaagt gaaccgaaag atccatgatt tacctccccc tggcatgacc
     1081 cgaattccaa ggccactgaa ctcgatttgt tcaaaccatt tttgctgggg ttttaaattg
     1141 cggatgtaag tatcggtaaa tttcataact ggtaccaaga tgatttttgg taccgttttt
     1201 ggtaccacta aatgtgaatt cagataatat taaatgatat taaaataaag tcaaatttca
     1261 ataaaaactc agtatttaca tgaaattatg atattttttg atattatttg aaatcaattg
     1321 atattgaata ataaatgact gttaatcatt aggtcacaag ttcgagtctt gtccggggag
     1381 ccaaatccag tctaggtttt ggcgaaattc aaataaaccc ttaaattttt ttgactaacc
     1441 ccctctgtgt gatggtgaaa cacctcttta cgtaagactt ctgtcgctcg ctggtattta
     1501 cctatcccag gaaaacgctg attaaaatag cgcttacacc catagcattg aaacttatgc
     1561 gacttgataa ttaaatagct ataacgcaat cctatcgatt catgacgtat cttgcgttca
     1621 aagctatcct tctttcttaa acgcttttca ttgcagtaaa tacatcgtac tactcggtta
     1681 taactcactt gtatataagc aggattgttt cctgtcacct tgactattga gtagccaggt
     1741 aaatttagga taatatcttt tcttggcact ttaagcctct tttgattgcg gaatcaaaga
     1801 gttagattac tataatccga ttaaagtacc cctatatttg gggtagagcc aaaatatcac
     1861 tagttgatct actgatttag ttcatatcta cgagcacttt ttaatgaaat ttgccttgat
     1921 atagaaacca atatcttttc aagcaaaggc gcgtggttta ctaatatcaa ggagaaaaaa
     1981 atgaatatac aaccaatcat gacctgatga gaaattgttt cgtgcaaaaa tatcatcccc
     2041 atgataaccc ctgttattgt cataaggtag gtaatcattg acgcaaaaac tgcacctgca
     2101 ttttggatta acgaacagta gagatataaa caaaatcctg atccaataac tcctaaccat
     2161 aacaaagcga ttatctgatg catgtcttga ggtattcccc agtagttaat tgaggattcc
     2221 gttgcacatt tgaaaataag cattgtaatt gcagaaacga atgccgaaac tgctgataca
     2281 agtattggat ctttagtacg agcatgatat ttgaaataat tagcagtaac tccgtatgaa
     2341 atggttgttg ctagaagagc tatgaattca acagctgaac cttgataaat actcacatct
     2401 gaattaataa taattattcc gataaatcca agcacgatac tcaatagatt caatacactt
     2461 aatttttgtt ttgaagaaaa aaacaccata cccaatatta aggtgcacag cggtgtaagc
     2521 ccattaataa cggatgctaa tgaactggat acagttttta ctgcataggt tgtcagataa
     2581 aaaggcagcg tcatgttaaa tgtcccaact attattccat ccagaattaa cccacgaata
     2641 ttatccgttt ttaatttaaa tagtttagcc aagataaaga gagaagctgc cccaattgcc
     2701 attcgataca aaacaatttc cgttggcgtt aaagacacca ataatatttt cattaaatag
     2761 agtgaaatgc cccagcccag tgttccaaga aacaaaaaac taaataaatt tgccataatt
     2821 aatacctaat aataatttta attgaataag taactgggga aatgacgaaa aacccgctgc
     2881 ttcgttgaga tgcccgcctt gagagatgat aatttttcta gcctgtagat tatttgcaat
     2941 aaaattaaag ttacttcttg atatgtaagg atcgttgctc ccatggtagc aaaaaaactg
     3001 tttggattta ttgattattg aatgccaatc aaacccattt tcaaaaaaac tttcattaat
     3061 tttatcaaat ttctgtatgc caacagctcc tataaacgga cctgctaaaa tagtgctaaa
     3121 tacagactga ttagcttgct caagccacct caataaaaat gccgatccca ggctatggcc
     3181 gattaaaatc gtatcatgag taatcattgg tgatactgta gaattaaata cgtgaagcca
     3241 gttctttaat tcctggccat ctggcgtagg cagttgtgga acaaaacact caatacctaa
     3301 actctctaat tcagtcttca tccaattgaa ccaattttca aaaggatgac cataggcacc
     3361 atgaacaata ataacattag attgagacat tcgattctcc tttattttta tcagttaaaa
     3421 tgaatgtctg aatcaatatc atgaagcatt ttagagagct tattataatc atcactttta
     3481 gcgatgacat gccccaagcg acgtaagggt ttaggaaact caggaattgc tacatgagca
     3541 ttatagtaaa tggcaatatc ttcaatagcc tgcatcttcg cagcatccat acctgttact
     3601 attgcaaaca tatctgtatg tggacgataa aaaaaacgaa tacctgcata attatttttc
     3661 atttctacta agggcatctt tatacccaga taggccgcat aaacataatc aaaataatta
     3721 ataccacggg ataaacaaag caaatcgcca atcttatcac ctgctaagcg ggctgcaatt
     3781 tccataagca ctggttcccc atcacgtcgc aatcgcactt cggcatgaaa tggacaattg
     3841 tcagcgccaa ggatttttat aacctgatca atatagtttt gaatctttgt atgcaactgc
     3901 tgttcaatgg gtgcattaac aatatgccct atttcgatga actcagattg atctgaaaca
     3961 aatttttcag tagtactaaa atgagcaata acgccatctt gcaccgttcc ttccaaactg
     4021 tattctttac catcgatata ctcttctaat agtaaagaac tagataattt atgcccccat
     4081 agaacatcat tgccatttaa tatccggtta gcagcctcga gcaattcttt tttattttta
     4141 acctttttaa cgtgaacgct tcctgcagca tcaataggct tacagatagc cggaaatcca
     4201 atataatcta ctgcttcatt aatatcatcc aaagaggtta ctaagtaata ccgtggacta
     4261 ttcaaacctg ctttatttaa agcagtccgc atgaggtctt ttcttctaag atttaatacg
     4321 tgtttacaat caatcccagg tactttcaat aaggcactga ctcttgaagc aataggaaca
     4381 aaatattcaa aacctggaat ggctgcatct atagacatat gctgctgtaa tgtaccagca
     4441 agctccaata cactgttatt atttgcagta tctatattga tattcagtgc agcctgattt
     4501 aaaaactcct caggcaccac tctaaaatca ctgttggctg ttaaaacaat aatattatac
     4561 tttttttcaa gtcctctctt tagcaatgaa agaacagcgc cataaggctc tataaaaaga
     4621 attgtttttt tggtcaagtc aaaattttta tattcttgta cgattaattt aatgaattct
     4681 ggttgtgtcg tgtagttaat taaatgaatg ctcatgtgca agttccttat gctatttttt
     4741 acgcaataca taaactggtt caaactgaca atactcatac tcatgtggat aaaatacttc
     4801 ctcaatatgg aaatagttag tgagtaaccg ctcaaatgca tcttggggag gacaataaaa
     4861 cttgctatta catcgtccaa atgtacctac aaaatcaaaa cttggcttga actgatccaa
     4921 ctgattgatt cctagctgtt caatagtatt ttgccaaccc atagcatagg aattagtgtc
     4981 atagcagata aagtttaatc tactggctaa gtcatactta gtcctaatca ctccctcttt
     5041 atactcacgc ctaacatcat ctattgtatt cgttataaag cattcctgtg gtttcactaa
     5101 taaatggcaa atgaataggc cattactttt aagaacacga ttggcattag ataagaacaa
     5161 gtcaaattga gtccactcaa ccatattaat cgcatcatca ccaatgagga agtcacatga
     5221 atcattagca attccctcta aattaagcca attggttaca acgagttcct cattggcagc
     5281 aataggtgct gcacccattg ttagatagcc catggcgcga acaattaact cagattgatc
     5341 cgttaaggtg acctttaaat ttgattcctg aaagagtgat ctgatttctg gtgttccacc
     5401 ataaattaaa aatgagtcct tattagagtt agattgaatt aaatctttat aataggcaca
     5461 ggtactaggt aaaggccgca cagtgtatcc tgcatatttt tcccattctt ctgcaattac
     5521 cttttcattt gaaacaagca tgtaacacat cattcacctc acgcggcttt tttcatgatc
     5581 aagctattca acaattggtc ataatatttt ttggtcgaat ccagtcgcat gtaaaaaccc
     5641 aaccaaaaat cctgcgtttt ataaggctta cgtgctagct ctggtatgac gacatggtta
     5701 agccacccac ttgcatggtg agcgtccact agttcgtgtt cggtgtaata ggagtaatcg
     5761 atattatgcg aactaaatat tctcataatg ccttttatta atttgttata atggggaggg
     5821 tcaagcatct ctgttgccgc aagcatgcca aagtacttag ttttattaaa aggatagata
     5881 gaaaagtaat tgaataaatt aattccagcc actccttccc aggacaggcc ttctaaaatt
     5941 tgttttcgat cataggttat acccaaatcg tttaaaaaat tttgaaacaa tacggtatga
     6001 aatttctcag cacatccttg gcctgcctca tcccaaaggt ttgccataat ttctaccttt
     6061 gcttgatcag atacccctat cactgctaat gctaagtagt caaagaattc caaattcata
     6121 attgattcgc tataggcgaa ttttttgagg tcctcaatgc tggcatcatg cattaaataa
     6181 tcaaagatag gatgattaaa tacctcatgt ttttgcaaca cgtctctaac aaga
//
LOCUS       DATNSN010000169         6172 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.169, whole genome shotgun sequence.
ACCESSION   DATNSN010000169 DATNSN010000000
VERSION     DATNSN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.169"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(350..2104)
                     /locus_tag="VAH76_002679"
                     /pseudo
     CDS             complement(350..2104)
                     /locus_tag="VAH76_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            complement(2183..2434)
                     /locus_tag="VAH76_002680"
     CDS             complement(2183..2434)
                     /locus_tag="VAH76_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1387268.1"
                     /translation="MSKNNVSRLQLINEFESASDSTLFNQNTLAAVLDCSVQLLERNR
                     WAGQGVPYMKIGRTVRYRKSDILNYLQQQCTYNVLKEAV"
     gene            2808..2984
                     /locus_tag="VAH76_002681"
     CDS             2808..2984
                     /locus_tag="VAH76_002681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387269.1"
                     /translation="MKYNLGMKILSNTNPELKDKENSDNLTLEAIEELESGGGKKYPS
                     FKSMIDDLEEDEKI"
     gene            3135..3581
                     /locus_tag="VAH76_002682"
     CDS             3135..3581
                     /locus_tag="VAH76_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1387270.1"
                     /translation="MTRLTTEIRNPSKHDRGIAKQSFQQIRQLSLPAKKTAFLDFEGV
                     HVELPSSAVKLVVTILNEIAEGNSLTLIPQHAHLTTQEAADMLNVSRPYFVKLLEEGK
                     IPFEKIGNRRRVLARDVMEFKEQERVKSEQALQELVDLAQKLDMGY"
     gene            3585..4154
                     /locus_tag="VAH76_002683"
     CDS             3585..4154
                     /locus_tag="VAH76_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEO1387271.1"
                     /translation="MVNFKVLYDACVLYPAPLRDLLMQLATCDLYRAKWSERIHQEWI
                     RNVLKNRPDLNIDTLEKIRVIMNKSVLDCLVENFEDIEQSLKLPDANDNHVLAAAIVS
                     GCDVIVTFNIKDFPNKELDKYGIEAQHPDDFLMHLTDLNTNKFCSAVKAVRTRLKNPP
                     KSIDDYLSTIEKQGLPKTAEFLVNYYSLL"
     gene            complement(4571..5806)
                     /locus_tag="VAH76_002684"
     CDS             complement(4571..5806)
                     /locus_tag="VAH76_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO1387272.1"
                     /translation="MKINKTNVESLPLPPVTEEGKTAQKRYYDDNLKGFGIRVTSGGA
                     KAFFVEKFINKKRCRITLGHYPELTAEMAKNKALHLLGQIAMGIDPIAEKKATEVRDI
                     TLNQVFKDYLKTRKNLKPRTIDNYKHILDKAFTSWKSKPMLSITRDRIAKYHEKLGNE
                     HGEAYANLAMRILRALFNFAAGQYEDSSGHSLFIDNPVKRLSQTRAWYRIERRKTYIK
                     SHELAAWYSGLQECQNETLRDYLLLILLTGLRRQEAATIKWSDLDLKAKTFTINKTKN
                     NESHTLPISDFLYDLLIVRKKKAINDYIFPGTGAAGHIIEPRKTIAHVIKNSGVQFTV
                     HDLRRTFITIAESLDIPAYALKRLMNHKMSNDVTAGYIIVDVERLRKPMQLITDYILK
                     CMEVIESADIINIQNSSKD"
BASE COUNT         1896 a         1173 c         1127 g         1976 t
ORIGIN      
        1 tgtctgaaaa ggatataaaa gagcttttag gtggctcaat tccgtattat tatgaagttt
       61 ttaaaccatt tattaattat aggcgtgcga aatataacag tgaaaattac ttttgtgagc
      121 ttgagcgtct aatatataaa ataaataaaa aatagtcttg tgtagactgg gttattatta
      181 agcccagaca atatagtctg tctgttattt tggaacagcc gaaactatat cgttgattta
      241 tcaacaatat aaaatttaag tggtacaaat gggacaagtg ggaaactctt actgttactg
      301 aagtgtaaat gttccactat caagattaat agtggaacgg gtgggacaat taaaactcgt
      361 cagaccagac tttctcagta aacacataac atctaggaat gccaactcct ttaatccttc
      421 gtttttgaga tgcttttcca tcattaccag gtactatcca gccagcatta ataagcactt
      481 tattcaccat tttaggttca aatccttggc agatctcctt cctataaacc tcagataaaa
      541 ccataaaaat tcgaaaacca gcgccatctg tgtgaaaaaa tccagctcta ttataaacgc
      601 gttcactatt aggatgattt tcgctttcaa atcgactcga tccttctttt tcaaagaagg
      661 cacgaacttg cgataggatg gctcgatcct ctctattgcc atgctcacca aaattttcta
      721 accatacatt gaagcatttc tccgctgctt ggcatgctgt acctcttttc caacctgtca
      781 aatcataatg agtagcgact tcccctgcca tagcaactaa agcaaaacgt ctagctactc
      841 gttggatctg gccagaatgt ccaggtttag tgacttttgt tacaaattgt tggattttat
      901 ttgcgagttg gggaattagt tctgtgcgat gctgaacaat cttgtagagc catgcaattc
      961 caactgttcc atgatattga ttcgaagcct cttttaatgc gatagccatt gaggcagggt
     1021 ttatataatc atgtaactgt tcaaagatgc ccatctgagc acctgcatcg gcctcaacat
     1081 cagctaaacg aatttcttga ccagcattgc atcgttgtcc agctttagcc attaatgcgg
     1141 ttaacgactc ttcgcctgct gataaaaaaa gtaattacca acgcattgat tgacgtgctg
     1201 taccacaacg agatgcgcgt gttttacctt gaccattagc aagtaaatag gcgcattctc
     1261 cggcttcttt aggatccatt tggctaagct catctaggat taacaatcca tcattatgaa
     1321 gagcagctaa tccctctaag ccatttgcag tgcttcgcca taagcgaata taactttctg
     1381 gcttacccca aacagaagct gctactttta aagcggttgt tttgcctgag gatgatgcac
     1441 ctctgaaatg aaatcctcca gaatcctcgc ctgtaagatc agctaggctt ggtgcgaatg
     1501 cagtggagat tgcaaatatt aaacgagaat taccttctgc taatcttgcg atagagttac
     1561 gccaatcctc aatgcttcct gctgtggtca atgccggttc taaactatta acattttgaa
     1621 aaacgatttg atcttctgat tggccaatgg cttcattagc ggtaacaaat acctctttat
     1681 accaacccaa tttatctaca caccgagcgc gatcttcaat aggaaaaacc tgaatgtagg
     1741 atgccaacaa ctcacgagct gttttgcttg gtgatattgc taatcctaat cgggctaact
     1801 cgcgtcttac atcactggaa tcgccttgta ataaagccaa gggcatcgcc cattggtgtt
     1861 taatgccatc atcatctagc cattcgagta atctacccca ttcaccacta tgagcgtctc
     1921 gcgttttagc tactacatac aaagaagagc aaatccagcg ggcgggtagc tcattattat
     1981 ctttgtcctt accaatgaaa atcactccat ctgaggttaa ttgaaaacat ccaccggcat
     2041 atgggaagga tttgatctgt ttagactgtt taaaatccac gtgatatatt tgattcgaat
     2101 tcatttcttt atctccaaca actataccgg aaggtgttgc accaccttcc aattaatcaa
     2161 taagacctag gttaagaaag tgctaaacag cttctttaag gacgttataa gtacattgtt
     2221 gctgaagata attgaggata tctgatttgc ggtatcgcac cgtacgacca attttcatat
     2281 aaggcacacc ctgaccagcc caacggtttc gttccaataa ttgaaccgag caatctaaaa
     2341 ctgcagcaag ggtattttga ttaaaaaggg tagagtctga cgctgattcg aattcattga
     2401 taagttgtaa gcgcgataca ttatttttag acatagtaat acctcgtcat taatagaatt
     2461 ttcaatcggc aataaaaatg ccgccaatgt ggctttttcc gtaaatccat catttgaaaa
     2521 tcccagtaac actggtactt tctatccaaa tatccatgta gctttatagc catattcata
     2581 aagctatttc atacaacgaa ctgattatta cgtgttttaa aaattaaagt caacaattga
     2641 aaacaaattc aaagaaattg taacttgagt gaacagattc gagtaatttg tcccacgcgt
     2701 gccacctgcg attttgacgg tggaacgctt ataactctgt aatatcaagg tgttccattg
     2761 gttccacttg taccactgaa aattattatt gctcgcatta tgtggaaatg aagtataatt
     2821 taggtatgaa aatattatca aatactaatc ctgagcttaa ggataaagaa aattctgaca
     2881 atctaactct tgaggctata gaagagctag aaagcggagg tggaaagaaa tatccatctt
     2941 ttaaatctat gattgatgat ttggaagaag atgaaaaaat ctgatttcat ggatcttcta
     3001 acgaacaaaa caactcttag ccttcagtgc acgatagaga tcactcgttc tacaatattc
     3061 gattatttcg actgtttcga ataattcgaa tctttgctat acttagaatt aagataatct
     3121 cttaggtatt tattatgaca aggctaacaa ccgaaattcg aaatccaagc aagcatgata
     3181 ggggtatagc taaacaatct tttcagcaaa ttcgccagct ttcattacct gctaaaaaaa
     3241 cagctttctt agattttgaa ggtgttcacg tggagttacc ttcctcagca gttaagttag
     3301 ttgttaccat tttaaacgaa atagcagagg gtaattcctt aacattaatc ccacagcatg
     3361 cacatctaac aacacaagaa gctgctgata tgctaaacgt atctcgacca tattttgtaa
     3421 aattactgga agaaggaaaa attccttttg aaaaaattgg taatcgccgc cgcgtcttag
     3481 ctcgtgatgt gatggagttt aaggagcagg aaagagttaa gagcgagcaa gcattgcaag
     3541 aactagtaga tctagctcag aagttagata tgggatatta gttcatggtg aattttaaag
     3601 ttttatatga tgcatgtgtg ctttatcctg ccccgttacg tgatttatta atgcaattgg
     3661 ctacatgtga cttataccgt gcaaaatggt ctgagcgaat tcatcaggaa tggattagga
     3721 atgttttaaa gaatagacct gatttaaaca tcgatacttt agaaaaaatc cgagtcatta
     3781 tgaataaatc ggtattggat tgtttggttg agaattttga agatatagag caatctctta
     3841 aacttcctga tgccaatgat aatcacgttt tagctgcagc aattgtatct ggatgtgatg
     3901 ttattgttac atttaacatt aaagattttc ccaataaaga acttgataag tatgggattg
     3961 aagcacagca tccagatgat tttttaatgc atctcacaga tctaaataca aataaattct
     4021 gttcggctgt taaagctgta cgcactcgtc ttaaaaatcc gcctaaaagt atcgatgatt
     4081 atttatcaac tatagaaaaa cagggcctgc ctaagacagc tgaattttta gtaaactatt
     4141 attcacttct ataaagttat cgataatcgg tttgctaaga ttcaattttc ccgaagaaaa
     4201 gaattttcca agggcgcaat atacattgct tcgcaatgtt tgctttttaa cacataaatc
     4261 cgggaaaagg attaagtacg tcacaattta atgataagca aaagaagcta aagaggctaa
     4321 ggatttattt tcggattatc attttcatta atgattgctt tataaatttg gcacacactg
     4381 tgtaccaaat tcattgtaaa aattgataga agactttccg aacacgatag ctgaaaacca
     4441 ttaatttggc ggacactgtc agccaaatgt gaaatatgga acgcaatgcg ttccgaatct
     4501 taaaaacttt gttatggatt tattctcaat tcggatcgca gtgcgatcca aaattagaaa
     4561 gttcttctgt ctaatcttta cttgaatttt gtatattgat tatgtccgca gactcaatca
     4621 cctccatgca ctttaaaata taatcagtaa tgagttgcat tggctttctc aatcgttcaa
     4681 catctacaat aatgtatcct gcagttacat cattactcat tttatgattc attaaacgct
     4741 tcaacgcata agcagggatg tccaaacttt ccgcgatggt gatgaaagtt cttctcaaat
     4801 catgaactgt aaattgaaca ccggaatttt ttatcacgtg tgctatagtc ttacggggtt
     4861 caataatgtg accagctgca cccgttcctg ggaaaatata gtcattaatt gctttctttt
     4921 ttctaacaat caacaaatca taaagaaagt cagaaatagg aagagtatgg gattcattgt
     4981 ttttggtttt gttgatagta aatgtcttag cttttaagtc gagatcagac cactttatgg
     5041 tcgcagcttc ttgtctgcgt aggccagtca ataagattaa tagtagataa tcacgtaatg
     5101 tttcattttg acattcctgc aaacctgaat accatgcggc tagctcatgc gatttaatat
     5161 aagtcttacg tctttcaatg cgataccacg cacgagtttg tgacagacgt tttacagggt
     5221 tatcaatgaa aagcgagtga ccactgctgt cttcatattg gcctgctgca aaattgaata
     5281 aagctcttag gattcgcatg gctagattag cataggcttc tccatgttca ttgccgagct
     5341 tttcgtggta tttagcaatg cgatctctgg ttattgataa cattggcttg cttttccagc
     5401 tggtgaaagc tttgtctaat atgtgcttgt aattatctat agttcttggc tttaagtttt
     5461 ttctggtttt taggtagtct ttaaagactt gattcaaagt aatatctcga acctctgttg
     5521 ccttcttttc agcgataggg tcaataccca tagcaatttg acctaaaaga tgtagggctt
     5581 tgttctttgc catttcagcg gttaattctg ggtaatgacc taaagtaatg cggcagcgtt
     5641 ttttatttat gaatttttca acaaaaaagg cttttgctcc tccagaagtc actcgtatcc
     5701 cgaaaccttt caagttgtca tcgtaatatc ttttttgtgc tgtttttcct tcttcggtga
     5761 ctggcggaag agggaggctt tctacgtttg ttttgtttat tttcataatt aatttttacg
     5821 tatgcttttt aatgtgtcgc acttgtgtcg cacaattgtt taaaatccat taatagaact
     5881 tataacgcaa tcatgctgta acatcaagta tataaaggga tattaaaaga atttcaaaga
     5941 gtgtctaatt ggctaaaacc cataattcac taattcgtaa tcaataggtc cgcggttcga
     6001 ttccgcgcaa cggcaccatt aaaatcaact ggttacatta aaaaacttcg ggggcgaatt
     6061 ttaactccaa gtgcgacaat catttttata ctgctgtata aacagttcgt tgggcgtttt
     6121 ataaccgata cattttctag ggcaccggtt caatttagct gctattaagt cc
//
LOCUS       DATNSN010000170         6153 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.170, whole genome shotgun sequence.
ACCESSION   DATNSN010000170 DATNSN010000000
VERSION     DATNSN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.170"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            53..580
                     /locus_tag="VAH76_002685"
     CDS             53..580
                     /locus_tag="VAH76_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387273.1"
                     /translation="MYLREKLVVILSCIFISSIVLAMPPVEFLQSCKNSKPANDSITM
                     TLLDGFGSTKDNEPGCRNHFDSEINGQLYGAVVCDDQPYLIIANKKISTATAKNYSMN
                     PSVKPDIPILRFSEWWRINKGDKSYLCIQTSLSDTGSAANSWQYYLVEDAFDVNKKNY
                     NISYYFFDMNVYKRF"
     gene            complement(986..1756)
                     /locus_tag="VAH76_002686"
     CDS             complement(986..1756)
                     /locus_tag="VAH76_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HEO1387274.1"
                     /translation="MKNEAIEKILNHQLIDLDLPALPAKHNHHGWEALYRLYFPEMPE
                     VNTNFYNAFSKAYAQIFRDGLNSSPMLQYRSKAVRSDKRLVYHVVSFCGSELKWADDL
                     LQNDKETVLAAVESDCNALEFASPLMQDDDDVVFKAIGNKRGFAIRYASPRLKENNDM
                     CQSAVEENGLALEHIPSQHRDLNLSLRALRSNFFASLYCTANVRKTIEYQKVHELTDY
                     QERNQLITFFLAKSSATKNARKTITAEEPNESIETLDL"
     gene            1873..3048
                     /locus_tag="VAH76_002687"
     CDS             1873..3048
                     /locus_tag="VAH76_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO1387275.1"
                     /translation="MPKKNCILNLPGYSIKKVSGENPVYIEVSYRRVVRCIHCGNKKL
                     RKKDSFIRRIRHESIGLRRSYLCIKAHKYYCPACGRYFNQRFPGIGKYQRASESLRKQ
                     VFHYHSKGVSQKDLARDLKLGKSTVERWYHYGYELRYKKIATRSCPRVLGLDEHSFNR
                     KVGYATTFCDLAKHKIFDVVEGRSEKDLEGYLNTLEGKDKVQVVCIDLSSSYRKLIRR
                     YFPNAMIVAVRFHVIRLLNQFCLQTYQQIDPEMKYQRGLLAALRTNPNNLSVKRLNKR
                     ERYLQQQPVIAAIYYFKQRLHRLLMRKHRTAKQCTRLIPLFLKLVASLKESPFESLKT
                     LGRTLYQWREEVVRMWRFTKNNGITEGFHRKMKLIQRRAYGFRNFENYRLGVKVLCS"
     gene            3115..4809
                     /locus_tag="VAH76_002688"
     CDS             3115..4809
                     /locus_tag="VAH76_002688"
                     /inference="COORDINATES: protein motif:HMM:NF036739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387276.1"
                     /translation="MEIESPQSLFDSAKDASELISLTEFFLAILNIFCRDQNLSEANF
                     GAILDRNKQLTQHLAEVVIKALKEHQSVEEALLKATCEKKRFFGISNWLDDSSLAKFK
                     RDFIKLYNQIQGSPQFDQFMLLSETKKGDFFVHHGSISMLFTRLLQTDDFAERLDDEQ
                     KAFITTAEANFDGVARDGNILPDTHAHIHAAIQGTNLDISQFSHAALRDLYEEICCYT
                     DPAIKQEALCQLQAGRPDFHPELDFAAFLKHVAYGQQRQAEQMLQKYQNFTQQLLRLD
                     TIPFIDRAGRTFTCTAYEYAMRVKDGHMLRILERYIVQDIETTRIIYERAQDVLYPEL
                     RYNSTFFGRLRNDAWHYTTCDNGVVKDHYEQFVGIAPLVSALEQLTYKVREMDASSAA
                     YTPADRAELTHIWVVGVGGAQRMLPEHMIQEYSQFGSERAFEQLLKNPQLLDSAHPKN
                     LKRRLREDEKTLWFSEALGSEYAIARGRINFPYSIDSAPDILGKAWGTSSEQELEAIR
                     LIDTIRTKDVYDSFNRLAELLPTNHEIACMRAAVLGTVCATGLKCYSLNRLHRRGI"
     gene            5372..6010
                     /locus_tag="VAH76_002689"
     CDS             5372..6010
                     /locus_tag="VAH76_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEO1387277.1"
                     /translation="MKSSEICPFGPKLQQYWDKRYDYFHRFDEGIQTDSEGLHTVMPE
                     DAALTQAKLFNNAKIVVDGFCGIGGSAIALARTGKKVIAIEMDSVRLAMAKNNARVYG
                     VEHLITFVHGDFFDVAAEIKADAVLIDPPWGWPRYRKIEEFRLQHFNPNGSKLLSYVL
                     DHFNKIVLRAPKTFITSELDQFNVNYEIHSDKLHHSIISISVCITNHSKRKS"
BASE COUNT         1882 a         1146 c         1265 g         1860 t
ORIGIN      
        1 aaactatatt ttggaagtac actaaagagg cgcttgaagc aggagaaacg caatgtattt
       61 aagggaaaaa ttggttgtaa ttttaagttg tatattcata agctctattg tattggctat
      121 gcccccagta gaatttttac aatcatgtaa aaacagtaag ccagccaatg attctataac
      181 catgacctta cttgatgggt ttggcagcac taaagataat gaaccaggtt gtagaaatca
      241 tttcgattct gaaattaatg gtcagctcta tggagcggta gtgtgtgatg accaacctta
      301 tttaataatt gcaaataaaa aaataagtac agcaacagct aaaaactatt cgatgaatcc
      361 aagtgttaaa ccagatatcc cgattcttcg tttttctgag tggtggagaa taaataaagg
      421 cgataaatct tatctatgta tacaaacctc tctctcagat acaggatctg ctgctaattc
      481 ctggcaatat tatctagttg aagatgcttt tgatgtgaac aaaaaaaatt ataatattag
      541 ctattatttt ttcgacatga atgtctataa gcgtttttga attgattaaa agctaattta
      601 ccagatatat aggatggaca aaggaaccca ctgacgtgcc taccgatcat agcccaattt
      661 tgtcccggtg gacacacttt gctttgttca tcctgcaaac tctcaaatct taaatataaa
      721 actctgcctg ttctgattta ccatcaaata acgtcatcaa ttcggataat aataaccgag
      781 ctgcattaat caaaaattct ttaaatttat cttttttcaa agcgcgggac tagggtcaga
      841 tcttgctttt tgcttcttaa aataaaaaag caaaagacaa gatctgaccc tgaacgctcc
      901 tggcttgcct tgcatgcttt acgggactat gggcagatta aatcaaaact aattcagatt
      961 aaatatagaa aattaatctg cttaattaca agtctaatgt ttctatggac tcgttaggtt
     1021 cctctgcggt aatagtcttg cgggcatttt tcgtcgctga gctcttggcg agaaagaaag
     1081 taataagctg attcctttct tgataatcgg tcagctcatg cactttctgg tattcaattg
     1141 tttttcttac attcgcagta caatacaatg acgcaaaaaa atttgagcgt aatgcgcgta
     1201 gagataagtt aagatccctg tgttggctag gaatatgttc caatgccaaa ccattttctt
     1261 caacggcaga ttgacacata tcattatttt ctttcaaacg tggagaagca taccgtattg
     1321 caaagcctcg tttattccct atggctttaa atactacatc atcatcgtct tgcatcaaag
     1381 gagaggcgaa ttctaatgcg ttacaatcgg attcaaccgc agccaatacc gtttccttgt
     1441 cattttgaag gaggtcatca gcccatttta gttcactacc acaaaaagat accacatgat
     1501 aaacaagccg tttatcgctt ctaacagctt tcgaacgata ttgcaacatg ggtgatgaat
     1561 taagcccgtc acggaaaatc tgtgcgtagg ctttgctaaa ggcattataa aaattcgtat
     1621 ttacctctgg catttctgga aaataaagtc gatataaagc ctcccagcca tgatgattat
     1681 gttttgcagg aagtgccggt aaatccaaat caatcaattg gtgattcaaa atcttctcaa
     1741 tagcctcgtt tttcaatagt ttgctcctta ttttatcgtt tataagtttt ggctctaccc
     1801 caaataaggg ggcattttaa tcaagttagc atagactaac tctttgattt gacggtcaaa
     1861 aggaagatta aaatgcccaa gaaaaattgt atcctaaatt taccaggata ttctattaaa
     1921 aaggtgagtg gagagaaccc ggtttatatt gaagtgagtt atcgccgggt agttcggtgt
     1981 attcattgtg gtaataagaa gttacgtaaa aaagacagtt ttataagacg catacgtcat
     2041 gagtcgattg gattaagacg cagttacttg tgtatcaagg ctcataaata ttattgtcct
     2101 gcgtgtggtc gctattttaa tcagcgtttt cctgggatag gcaagtatca aagagcgagc
     2161 gagagtttgc gtaaacaggt atttcattat cacagcaaag gagtgagtca aaaggatttg
     2221 gctcgtgatt taaagctggg taagtcgact gtagagcgct ggtatcatta tggttatgag
     2281 cttcgttata agaagatagc gacacgttcc tgtccccgag tattaggact tgatgaacat
     2341 tcatttaatc ggaaagtggg ttatgctaca accttttgtg acttggccaa acataaaata
     2401 tttgatgtgg ttgaggggcg ttcagaaaag gatttagaag gttatctcaa cacgttagaa
     2461 ggaaaagata aggtacaggt agtctgtatt gatttaagtt ccagttatcg caagttaatc
     2521 aggcgttact ttcccaatgc gatgattgtt gccgttcgct ttcatgtgat acgtttgttg
     2581 aatcaattct gtttgcagac ttatcagcag attgaccctg aaatgaaata tcagcgaggt
     2641 ctcttggcgg cattgagaac caatcccaat aacctcagtg taaaacgatt aaataaacga
     2701 gagcgctatt tgcaacaaca gcctgtcatt gctgcaatat attactttaa acagcgatta
     2761 catcgattac tcatgcgaaa acatcgcacc gcaaagcagt gtacgcgttt aatccctctc
     2821 tttcttaaac tggttgccag tttgaaagaa agtccttttg aatcactcaa gactctgggt
     2881 aggacattat atcaatggcg ggaagaagtg gttagaatgt ggcggtttac taaaaacaac
     2941 ggcattactg aagggttcca tcgtaagatg aagctcattc aacgtcgtgc ttatggattt
     3001 agaaactttg aaaattatag gttaggggtt aaagttttat gttcttgatt agtgctcccg
     3061 gatttgggga agagccgata taatttgtaa ccgatgtctt cggaatagac ctgcatggag
     3121 attgagtcgc cgcaatcgct tttcgactcg gccaaggatg cgtcggaatt aatcagttta
     3181 acggaattct ttttggcgat cctgaatata ttctgtcgtg atcaaaattt atcagaggct
     3241 aattttggtg caatattaga taggaataag caacttactc agcatctggc ggaagtggtt
     3301 atcaaggcct taaaagaaca tcaatcagtt gaagaggcct tgttaaaggc cacttgtgag
     3361 aaaaaaagat ttttcggaat atcaaattgg cttgatgaca gcagcctggc caagtttaaa
     3421 cgagatttta tcaagcttta caatcaaatc cagggatcgc cccaatttga tcaatttatg
     3481 ctattaagtg aaacaaaaaa aggagacttt tttgttcatc acggctctat atctatgtta
     3541 tttacacgac ttctgcaaac agacgatttt gccgaacggc ttgatgatga acaaaaggca
     3601 ttcattacga cagcagaagc aaattttgat ggggtagcta gagacggaaa tattcttcct
     3661 gatacacatg cccacattca tgctgcgatt caaggcacca acctggatat ttctcaattt
     3721 agccacgcgg ccttgcgaga tctatatgag gagatttgtt gttatacaga cccagccata
     3781 aagcaagagg cgttgtgcca attgcaagca ggacgtcccg atttccaccc tgaactggac
     3841 tttgccgctt ttctaaagca tgtcgcttac ggtcagcagc gccaggctga acagatgctg
     3901 caaaaatatc aaaattttac acaacaactg ttaagattag atacaatccc ctttattgat
     3961 cgcgcgggac gaacgtttac ctgcacggcc tatgaatacg cgatgcgcgt gaaagatggt
     4021 catatgctca ggatattgga gcgatatatt gttcaagata tcgaaacgac cagaattatt
     4081 tacgagcgag ctcaagacgt gctttaccca gagctcaggt ataattcaac attctttgga
     4141 cgcttgagaa atgatgcctg gcattataca acctgtgata acggtgtagt taaagatcat
     4201 tatgagcaat ttgttgggat cgccccctta gtgagtgcat tggaacaact aacttataag
     4261 gttagggaaa tggatgcaag ctccgcagca tatactccag ctgatcgggc tgaactgact
     4321 cacatctggg tagtgggagt aggtggggcg cagcggatgt tgcctgagca tatgatccag
     4381 gaatattctc agtttggctc tgaacgtgca tttgaacaac tattgaaaaa tccccaattg
     4441 ctggatagcg ctcatccaaa gaatctaaag cggcgcctga gagaagacga aaagacatta
     4501 tggttttctg aggccttagg ttcagaatac gcgatagctc gcggcagaat taacttccct
     4561 tatagtatag attctgcccc ggacattttg ggcaaggcat ggggtacctc ctccgagcag
     4621 gagttagagg cgattagatt aatagataca atcagaacta aggatgttta tgattccttt
     4681 aaccggctag cagaattgtt gccgacaaat catgaaattg cttgcatgcg tgcggctgta
     4741 ttaggaactg tctgtgcaac cggtttaaag tgttacagct taaaccggtt gcacagaaga
     4801 gggatttaaa ggtaaaacat tataccaatg tgttgtaagg gtcagatttt gccctttgct
     4861 cccattgtgt tgatttataa cttttgtaat tcttgagtat agaggcaaaa ggcaagatct
     4921 gacccttatt cagcacagtg actccgttca gcttaaaagc tattaactcg ccataatcat
     4981 aaattaccag atgaggcttg aagacaaaaa actaccctat tgacgatttt cccttttttg
     5041 caatccgttc aaatttaaat gttttacgtc aatagctgtt gcatccacaa aataaatacc
     5101 tgttctctac ttgaattatg ttacaaggca cgatagccta tcaaagctta atagttagct
     5161 taatcagcaa ctctcgatcc ctccggtgct gatgtaataa gtggctatgt gtcgagggat
     5221 acttaaatat aacaatattg aggtatcctc catgcagaac gtcaatcaaa caactctatt
     5281 ccatacaaac aacaaggaaa ctctaaaatt ttcaatcctc gattatgcag gcaggcttgc
     5341 tatatggggt gcactttgat acaggtaaaa aatgaagtca tctgaaattt gcccatttgg
     5401 ccctaaatta caacaatatt gggataagcg atacgattat tttcatcgct ttgatgaggg
     5461 gattcaaaca gactctgaag gtttgcatac cgttatgcct gaagatgctg cgctcactca
     5521 agccaaatta ttcaataatg cgaagattgt agtagatggt ttttgtggta ttggcgggtc
     5581 ggctattgct ctggcaagaa caggtaaaaa agtcattgct atcgaaatgg attccgtccg
     5641 tttagcaatg gctaaaaata atgctcgtgt ttatggtgtt gaacatctta taacgtttgt
     5701 tcatggagat tttttcgatg ttgctgctga gataaaagct gatgctgtcc ttatcgaccc
     5761 gccatgggga tggcctcgtt acagaaagat agaagaattt cgtttgcagc attttaatcc
     5821 gaatggaagt aaattactca gttatgtgct tgatcatttc aataaaattg tattacgtgc
     5881 accaaaaaca tttatcactt cagaattaga tcaatttaat gtcaactatg agattcattc
     5941 tgataagctt catcattcaa tcatttctat ttctgtatgc atcactaacc attccaaaag
     6001 gaagtcttga tagtgttttt aaataccaga tgagtccata tcattgcttg ctgtccaacc
     6061 caagtcccaa taatactccc ttcattattg ggggattggt gtactataca ttgaacaatc
     6121 cagttaagaa atttagtcct tttggtttat ttt
//
LOCUS       DATNSN010000171         6116 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.171, whole genome shotgun sequence.
ACCESSION   DATNSN010000171 DATNSN010000000
VERSION     DATNSN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.171"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..565)
                     /locus_tag="VAH76_002690"
     CDS             complement(<1..565)
                     /locus_tag="VAH76_002690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387278.1"
                     /translation="MDLSIIINTIATLATVFISFVLSKYLYNRAKLVTYCLHASAHKI
                     KDPAGIINTHAIVIRNAGRIAAKNVRVGHYVFPELSCTVTPPIEYEKKEIEGGTELIF
                     PTLVPQEQIVISYLYFPPITIRDVNTYVKSDEGFAKILNIIPSPKLPGWLEKILYILL
                     FIGGVVVMRIIIQLIIFIFQSVSHFCVF"
     gene            complement(587..850)
                     /locus_tag="VAH76_002691"
     CDS             complement(587..850)
                     /locus_tag="VAH76_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HEO1387279.1"
                     /translation="MTTHRLIEKEKQLIAQCEKSIALEKLKKRRADTRLKIELGGLII
                     KSGMNHYNKAIILGALEHVSQLMRQDECYIKLFEISGESLFIK"
     gene            complement(837..1043)
                     /locus_tag="VAH76_002692"
     CDS             complement(837..1043)
                     /locus_tag="VAH76_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387280.1"
                     /translation="MSTKTVSQMEAVLLDRIEKAKIKLDKLQQKHKLEIGTLAYKHGL
                     HQFDTKRLDAAFLKLAGELRHDNS"
     gene            1291..3936
                     /gene="traA"
                     /locus_tag="VAH76_002693"
     CDS             1291..3936
                     /gene="traA"
                     /locus_tag="VAH76_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HEO1387281.1"
                     /translation="MAIAFAQVSIHSRAKGHSAIAASSYRSGAKLYDARTGITHDYSN
                     RHDVIYSDILLPEGSPEAFSEREFLWNKAELAEKRCDAQVCKDIVLALPKELDLVQQI
                     ELARRFAQTHFVDKGIPADITIHDHHDGNPHAHILITTRRLEKTGFSKYKARDLNPAF
                     AKGFIIEKDYWGEQWRDMQNEYFIEKNLDLTVDLNHIISERHHGKLKDTDNHYLLTEK
                     TILQQARQEVLLNDIDNVINHISAQNSVFTRRDVERLVFKTFRPSNTPQNYLHWVEQI
                     MGHEDLIELGTNERGQLCYTTRHQYIQEAKLRDDIEAMIKNKDGIGGKGIDNIIKNYP
                     LSDEQLEAVRYITGGSQISVVIGRPGTGKSYLLKPIKEHYEQHNYRVIGAALSGKVAK
                     SLQTDTGIASSTIASLTYKMANQQLKLNSNDVLIIDEAGMVDFSSMALLIREAKKAGS
                     KVILVGDPDQLKPIHKGEIFRGIAALTGYIELEHIKRQNDLGDRLASMNMAKGMIAEA
                     VDHYHEKGAIVFSETTETAAQNLIHDWQADITKSNLQDSVVLAFTRASVGYLNEQARL
                     ALKEKQILGQEEMTFQGFERLLKIAIGERLLFRQNDKTLGVRNGDLGTVQSIKKDQFQ
                     IKLDSGELLTIPNSYNKIDYGYALTVHKSQGMTVRHSKVLIDSKYWDRHLSFVAMTRH
                     KDSLKIYTDSINHPTIKELKQTLSRSTTKDNVIDWPLDFATRVGFNPDQLIGRVLNHI
                     AGVGHKIKSAFNYVVHYEAYLVDCQLRELKNDKADLKAKARLAADRQDRPTQEITTSN
                     SKENVYKELQKNYPILAQYQKLLEKRRYLNGYFAEKTGKQISEHAHILSKDKPLMNQL
                     NIHLPQIAKKISEVSKIVKIQDLVD"
     gene            complement(4262..5449)
                     /locus_tag="VAH76_002694"
     CDS             complement(4262..5449)
                     /locus_tag="VAH76_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEO1387282.1"
                     /translation="MIFGILNLTFWGYVLATFVLTQITIASVTLYLHRYQTHRALTMH
                     PIVSHFFRFWIWLTTGMVTRVWVAIHRKHHAMTDVEGDPHSPQIFSLKKVFWQVPELY
                     REAAKDKDMIEKYSHGTPNDWIERNLYSRYSSKGILLMFLIDLLLFGFPGITIWAIQM
                     IWIPLSAGVVNGVGHYWGYRNFECFDRSTNVFPLGFLIGGEELHNNHHTYASSAKFSV
                     KWWEFDLGWFYIRCLSFLRLAKVKKLPPKLIRDNSKQYVDLNTIKAITSNRFQVMSDY
                     YKRVTIPILKNEKQNYNYIKNKKEWYLFKGAKRLLRQETKKANYHLLALLNRFEQLQL
                     VYNYRQSLQQIWLKKVSSQNELIESLNQWCKKAEESGLDVLRQFSLRIKSYVLIGTDS
                     SSV"
     gene            complement(5581..5748)
                     /locus_tag="VAH76_002695"
     CDS             complement(5581..5748)
                     /locus_tag="VAH76_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HEO1387283.1"
                     /translation="MLGTILLILLILLLLGSVPRWGYSRSWGYTPMGLIGLILVIYLI
                     LVLLGKVPIGF"
BASE COUNT         1981 a         1164 c         1159 g         1812 t
ORIGIN      
        1 aaaaaacgca aaaatgacta acactctgaa atataaaaat tattaattgg attattattc
       61 gcatgacaac cacgccacca ataaacaaaa gaatataaag tattttttcc aaccatcccg
      121 gtaacttggg tgaaggaata atgtttaata tttttgcaaa cccttcatct gatttgacat
      181 aagtattaac atcacgaatt gttataggtg gaaaatataa atatgaaata actatttgtt
      241 cttgaggaac taaagtagga aatattaatt cagttcctcc ttcaatttct ttcttctcat
      301 attcaattgg tggggttact gtgcagctta actcagggaa tacataatgc cccacacgaa
      361 cattttttgc tgcaatccta ccggcatttc ttataactat agcgtgtgta tttattatac
      421 cggcaggatc tttaatttta tgagcagatg cgtgtaagca ataagttact aattttgctc
      481 tattatataa gtacttactt aatacaaaag atatgaatac tgttgctaag gtagcaatgg
      541 tattaataat gatggaaaga tccatataat gacatactcc aaagatttat tttataaata
      601 ggctctcgcc gctaatttca aataatttta tataacattc atcttgacgc attaattggg
      661 aaacgtgctc caatgcccct agaattattg ctttattata atgattcatg cctgatttga
      721 taattaaccc tcctagctca attttaaggc gagtatcagc tcgacgtttt ttcaattttt
      781 ccaatgctat tgatttttca cattgagcaa tcaattgttt ttctttctca attaatctat
      841 gagttgtcat gtcgcaactc accagcaagt tttaaaaatg ctgcatccaa tcgcttagta
      901 tcaaattgat gaaggccatg tttgtaggca agagtaccaa tctcaagctt atgtttctgc
      961 tgcaacttat ccagcttgat tttggctttt tcgattctat ccaagagtac tgcttccatt
     1021 tgcgatactg tcttcgttga cataatcatt tctccgcaaa tgaggtaaca gcccaagcgt
     1081 acaatgccag atcattaaaa tcaataggtt gttccatgtg aagtgagcga agcgaacaaa
     1141 atgaatcccg aagcgaaaag caagggcgca atatacattc gttcgaatgt ttgctaaatt
     1201 agaactgcga ctcaaaaatg cctttatgga taaggtattt tttcttttat taaagcaacc
     1261 gatggatcag ttcgattaga gcgtgaattc atggcgattg cttttgccca agtatccatt
     1321 catagtcgtg ccaaaggcca cagtgccatt gctgctagca gctatcgttc tggtgccaag
     1381 ttatatgatg cacgcactgg cataacacac gattattcca atcgtcatga tgtcatttac
     1441 tctgatatcc tgttacccga agggagccct gaagcatttt ccgagcgtga atttttatgg
     1501 aataaggcag agcttgctga aaaaagatgc gatgctcaag tgtgtaagga catcgttctg
     1561 gcacttccca aagagctgga tttggtgcaa cagattgagc tggcaagacg atttgcacaa
     1621 actcattttg ttgataaggg gattcctgct gatattacaa ttcatgatca tcatgatggc
     1681 aatccccatg cacatattct tattaccacc cgccgtttgg aaaaaacagg tttttcaaaa
     1741 tacaaagcac gcgatctgaa tccggctttt gccaaaggat ttattatcga aaaagattat
     1801 tggggcgagc agtggcgtga tatgcaaaat gaatatttta ttgaaaaaaa tctcgatttg
     1861 acagttgacc tgaatcacat catttctgaa cggcaccatg gcaaactaaa agatacggac
     1921 aatcattacc tactcaccga gaaaaccatt ctgcaacaag cgcggcaaga ggttttgctg
     1981 aatgatattg ataatgtcat taaccatatt tcggcgcaga acagcgtatt tacccgacgt
     2041 gacgtagagc gtttggtatt taaaaccttt cggccaagca acaccccaca gaattatttg
     2101 cactgggttg agcaaatcat ggggcatgag gatctcattg agcttggaac aaatgaacgg
     2161 gggcaattgt gttataccac acgtcaccaa tatattcagg aggcaaaact tcgggatgat
     2221 atcgaagcta tgattaaaaa caaagatggt attggcggta agggcatcga caacatcatc
     2281 aaaaactacc cattgagtga tgagcaattg gaggcggttc gttacattac cggaggctct
     2341 caaatttcag tcgtgatagg tcgtccgggt acaggtaaaa gttatttgtt aaagccgatt
     2401 aaagagcatt atgagcagca caactatcgt gttatcggtg ctgccttgtc tggaaaggtc
     2461 gctaaatcct tgcaaacgga tacaggcatt gcatcttcta ccattgcctc attgacttat
     2521 aaaatggcga accaacaact gaaactcaac agcaacgatg ttttgattat cgatgaggca
     2581 ggcatggtgg atttttccag catggcactg ctgattcgtg aagccaaaaa ggcaggttcc
     2641 aaggtgattc ttgtgggcga tccggatcag ttaaaaccca ttcacaaggg tgaaattttt
     2701 agggggatag ccgctttaac aggctatatc gagcttgagc acattaaacg tcaaaatgat
     2761 ttgggcgaca ggcttgccag tatgaatatg gctaagggca tgattgctga ggcggtagac
     2821 cattaccatg aaaagggagc aattgtcttt tctgaaacga ctgagaccgc agcccagaac
     2881 ttaatccacg attggcaagc tgatattacc aagagtaatt tgcaagattc tgtcgtgttg
     2941 gcatttacca gagcttctgt tggctattta aatgagcagg cacgattggc attaaaggaa
     3001 aagcaaatac ttggacagga agaaatgact tttcaagggt ttgaaagact gttaaaaata
     3061 gccatcgggg agcggctgct attccggcaa aatgataaaa cccttggggt aagaaacgga
     3121 gatttaggca ccgttcaatc catcaaaaaa gaccagtttc aaatcaaact cgacagcggc
     3181 gaactgctca ccattccaaa tagctacaat aaaatagatt acggttatgc gctgacggtt
     3241 cataagtctc agggaatgac cgtcaggcat tccaaggttt tgattgacag caaatactgg
     3301 gatcggcatt taagttttgt agccatgact cgtcataaag acagccttaa aatttatacg
     3361 gactctataa atcatccaac aattaaagag ttgaaacaga ccttatcgcg cagtaccaca
     3421 aaagataacg tcattgattg gccactggat tttgcaaccc gtgtcgggtt taacccggat
     3481 cagctcatag gtcgtgtcct caatcatatc gctggcgtgg ggcacaaaat caagagcgca
     3541 tttaactatg tggtacatta cgaggcttat cttgtcgatt gccagcttcg agagttaaaa
     3601 aacgataagg ccgatttaaa ggcaaaggct agactggctg cggatagaca agatcggcca
     3661 actcaagaaa taactacaag taactcaaag gaaaatgttt acaaagagtt acaaaaaaat
     3721 tatccgattc ttgcccaata tcaaaaattg cttgaaaaac gaaggtattt aaatggctat
     3781 tttgccgaga aaacaggtaa acaaatttct gaacatgccc atatattatc aaaagataag
     3841 ccattgatga atcagctaaa tattcattta ccccagatag caaaaaaaat cagcgaggtt
     3901 agtaaaattg taaaaataca ggacttggtt gattaaacgc caagtaccgt cgttttatcc
     3961 ttagagagga gttagcaaaa cttttggatc gtagctctta agcgtctaac ctcaactctt
     4021 gttttgcaat ttctgttaca tctatgcgaa gaaacatata gtgaaaccat tttttgcgat
     4081 taccacgatt ttcattaatg tagttgaggt gtaaaaactt tcttgatcac ttcaatcgtc
     4141 aactcctaaa gttataaaaa cattcaatgc gtgcgtttta ttcacgtttt tttgattagt
     4201 accaatactt atcaattgca tagtataaac cacgaaatcc tattcacttt tgttgcagag
     4261 cttacactga tgagctatct gtcccaatta aaacatagct ttttattcga agagaaaatt
     4321 gacgcaatac atccagacct gattcttccg cttttttgca ccattggttt aaagattcaa
     4381 ttaactcatt ttgagatgat acttttttta accaaatttg ctgcaatgat tggcgataat
     4441 tataaacaag ctgcaattgc tcaaaacgat tgagcagggc tagcaaatga taattagctt
     4501 ttttggtttc ttggcgtaat aaacgtttag ctcccttaaa taaataccat tcttttttat
     4561 ttttaatata gttatagttt tgtttttcat ttttcaaaat gggaatagta acccttttgt
     4621 agtaatcgga cataacttga aaacgattac tagtgatggc ttttatcgta tttaaatcaa
     4681 catattgttt gctattgtct cttatcaatt tgggaggtag tttttttacc ttggcaagcc
     4741 ttaaaaaaga taggcaacgt atataaaacc agcccaaatc gaactcccac catttcacag
     4801 aaaatttagc agacgaagcg taagtgtggt gattattatg aagttcctct ccacctatta
     4861 gaaagcccaa aggaaataca ttggttgacc tatcaaagca ttcaaaattg cgatatcccc
     4921 aataatgccc cactccattt actacacctg ctgagagcgg aatccaaatc atttgtattg
     4981 cccaaatagt aattccagga aaaccaaaca gcagcaaatc aattagaaac ataagtagta
     5041 tacccttgct ggaatagcga gagtacagat tgcgttcaat ccagtcgttt ggagtgccat
     5101 gagaatactt ttcaatcata tctttatctt ttgctgcttc gcgatacaat tcaggaactt
     5161 gccagaagac ctttttaaga ctaaagattt gggggctgtg agggtctccc tcaacatctg
     5221 tcattgcatg gtgtttacga tggatagcaa cccagactct tgtcaccata ccggttgtta
     5281 accaaatcca gaatcgaaaa aaatggctca ctattgggtg catggttaaa gcacgatgag
     5341 tttgataacg atgcaaataa agagtgaccg aagcaatggt aatttgagtt agaacaaaag
     5401 ttgctaaaac atatccccaa aacgttaggt ttaaaattcc aaaaatcata gggaagcctc
     5461 attgtctgta cttaagcaac cccccttttt ccacattatt ctcaaaactg gatatttttc
     5521 cttttctcca agtttgaatt cattacatgg cttattctag ttgtttaagg ctaattccat
     5581 ttagaacccg ataggtactt tacctagtag aacaagtatc agatagataa ccaggataag
     5641 tccaataaga cccatcggtg tatatcccca gcttctgcta tatccccatc tcggtacgct
     5701 acctaaaagt aacaggatga gcagaattag taaaattgtt cctaacatga cggctttcct
     5761 taaagaaacg gttcatactt taattatagc ataattaaag tccaataagt tcatttattg
     5821 gtctataatt aaaagaatgg gtgtaccggt tgataaatca tggaaaataa acaaataagg
     5881 gaatctcttc aagaaattat taggtatttg gagaattatg aagagaagga acgcttgcca
     5941 gaaaatccct cccatggtct taaacaaaca cagttttcct caataaggga tgtcgtgcgt
     6001 aagttaaatg aaattcttaa ttctatacgt taattttatg cataacctaa ccacaccaga
     6061 agttaattaa cgtgaattcg atataactgt ctaaaaaaca aacttaagaa tggtaa
//
LOCUS       DATNSN010000172         5977 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.172, whole genome shotgun sequence.
ACCESSION   DATNSN010000172 DATNSN010000000
VERSION     DATNSN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.172"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(28..537)
                     /locus_tag="VAH76_002696"
     CDS             complement(28..537)
                     /locus_tag="VAH76_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEO1387284.1"
                     /translation="MESNNTQQSIKTHSSVIVLYDLSKDSLILTKRSQMLNKHPGEVC
                     FPGGFQEINDQDLYSTALRELNEELGVTSDRITLIRKLNIERTLLGLVIHPWYASIDS
                     IFPYTMNPQEVSKLILVPMPLVKNQKNYKEIRIEKEGKHIVTCQFIANEELIWGATAR
                     IMRQLASQY"
     gene            complement(524..1846)
                     /gene="pabB"
                     /locus_tag="VAH76_002697"
     CDS             complement(524..1846)
                     /gene="pabB"
                     /locus_tag="VAH76_002697"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO1387285.1"
                     /translation="MIDSTLITLDYHDFSSESYEKLSRLPGFVLLQSTDKSTGRYDIL
                     SAHPYERMVIENNVYDKDLVLTKLKNKLKIEQSEFDLPFQGGAIGYVSYDFGALLHDH
                     ELYPQKDLAHIPVLNFGFYDWAIIVDHHLKKATLFARNSHSSTKSVVDEIKNILNNQS
                     TKSVQFSVQSEFLPLISRDNYRNSFNAIQQALRTGRSYQVNFTQPFQTKFTGDPWDMY
                     QRVNKNNPVPYAAFIRTEEADVLSFSPERFLLYDNGEMLTSPIKGTIHRSKDPVVDKK
                     LKEQLLNCPKNRAENVMIVDLLRNDLGKLAKADSVKVMSLCNIQSFNAVHHLVSDIRA
                     LCKEDIHPIEAFFSCFPGGSITGAPKLETMKIISEQETYKRGVYCGCIAYFSQHGRFD
                     ANIAIRTVTAKDNVLYLAAGGGIVIDSDWEDEYRECLTKISAIINGIK"
     gene            2026..3099
                     /gene="pdhA"
                     /locus_tag="VAH76_002698"
     CDS             2026..3099
                     /gene="pdhA"
                     /locus_tag="VAH76_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEO1387286.1"
                     /translation="MTTVAQFEITYTQYLNEQGKLVAELPPFADNQSILKELYKIMVL
                     TRTFDKKAIALQRTGKMGTYAPINGQEAISTAIGHALKPEDVLVPYYRDYAAQFQRGV
                     KMSEILAFWGGDERGSHYSNNAEDLPICVPIASQCLHAAGVAFAFKYRNQPRVAVVCI
                     GEGGTSEGDFYEAMNVAGAWKLPVVFVVNNNKWAISVPIEKQTGSQTIAQKAIAAGFS
                     GVQIDGNDIFAARQVIGDAIEKARQGGGPTLIEALTYRLCDHTTADDATRYQPSHEVE
                     EAKAKEPIVRFKHYLMQQNIWTSQDEEKLVIECSEKVEKAVEEYLNTKPQPVSSIFDY
                     HYAELPEYLIEQRAIAMEEYSHA"
     gene            3092..4066
                     /locus_tag="VAH76_002699"
     CDS             3092..4066
                     /locus_tag="VAH76_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO1387287.1"
                     /translation="MPDITLVEAVTQALAYELAHDENVVVFGEDVGKNGGVFRATVGL
                     QDRFGENRVFDTPLAESMIAGLAVGMSIQGLKPVAEFQFMGFIYPAMNQIISHAARMR
                     NRTRGRLHCPLVYRAPFGGGIRAPEHHSESTEALFAHIPGLQVVIPSSPKRAYGLLLA
                     AIRNPDPVIFLEPKRIYRLVKQPVPDDGQALPLGKCFTLQQGDDLTLISWGASMHETL
                     QAAKQLTDEGISCDVIDVATIKPLDIETILSSVEKTGRCVIVHEGAKTCGVGAEISAQ
                     IMEHCMADLLAPVQRVTGYDTVMPYFQLEKQYIPSIARIKNTVMSIME"
     gene            4069..5181
                     /locus_tag="VAH76_002700"
     CDS             4069..5181
                     /locus_tag="VAH76_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEO1387288.1"
                     /translation="MNIFNLPDLGEGLPDAEIHEWFVKEGDTVKADQPLVSMETAKAV
                     VDVPCPQSGTIAKLYGKPGDVIKTGEPLVAFVSTTEKPADKGTVVGNLEESTDVSEDN
                     FIIGSQRSSHRVKTTPAVRMLAKKLGVDLSSLKGSGDNGVITREDVQNQANINSQPPA
                     GFEPLRGVRRAMLNSMVQSHAEIVPVSIFDEADIHIWKPNTDITVRLIRAIIHASTKE
                     PALNAWFDTKHAARRCFKEVHLGIAMDNEEGLFVPVIHDAEKYSDSELRKIIDEFKES
                     VRNRAVSADKLKGATITLSNFGKFSGRFASPIIVPPMVAILAVGRLYKAAVINDEDKI
                     EAHKMLPLSLSFDHRAITGGEATRFLGAVIESLQQA"
     gene            5368..>5977
                     /locus_tag="VAH76_002701"
     CDS             5368..>5977
                     /locus_tag="VAH76_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEO1387289.1"
                     /translation="MAMRIFVMILLFFFSISPTLGDTTLPAIFDVKNANKQFDKINLQ
                     LSVQNLNLNNLNKAVETLSELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLV
                     YLNNQKHQLAELQSQCRLFSIRAQEAIDAYKSAILQLRQEKTLARTLPLWQLVSKIIN
                     SSDNTKWINHTKEKFLLQDLLVYLWFCIVTGSFFISILILSKL"
BASE COUNT         1838 a         1074 c         1192 g         1873 t
ORIGIN      
        1 atgcctttta tcatcctggt atcatactta atattgagaa gctaattgcc tcataattct
       61 tgctgtagca ccccatatta attcttcatt agcaataaat tggcaggtga caatgtgttt
      121 tccctctttc tcaattctaa tttctttata atttttctga ttttttacta aaggcatagg
      181 cacaagaatt aatttactaa cctcttgtgg attcatagtg taagggaata tagaatcaat
      241 actggcgtac caagggtgaa tgactaaacc cagtaaagtc ctttcaatgt ttaattttct
      301 gatcaaagtt attctgtctg atgtaacacc taactcttca ttaagttctc tcaatgcagt
      361 gctatataga tcttgatcat ttatttcttg aaatccacca ggaaagcaca cttctcctgg
      421 gtgtttatta agcatctgac ttcgcttggt gagaattaac gagtctttgg aaagatcata
      481 taaaacgata actgatgaat gtgttttgat tgactgttgg gtattatttg attccattga
      541 taattgcact aattttggtt aaacattcac gatattcatc ttcccaatcg gagtcaataa
      601 ctattccccc tcctgctgcc aaatacaaaa cattatcctt ggctgtcacc gtacgtatcg
      661 cgatattggc atcgaaacgg ccatgttgtg aaaaataggc tatacaacca caatagaccc
      721 ctcttttata ggtttcctgt tcactgataa ttttcatcgt ttctaattta ggcgccccgg
      781 taattgaacc gcctggaaaa caggagaaaa atgcctcaat tggatgaata tcctctttgc
      841 aaagagctct tatgtcggaa accaaatgat gtactgcatt gaagctctgg atgttacaaa
      901 gactcattac ttttacgctg tcagccttgg caagtttccc tagatcgttt cttagcaagt
      961 cgacaatcat gacattttca gccctgtttt taggacagtt taataactgt tcttttaatt
     1021 ttttatctac aacaggatct ttcgatcggt ggatagtgcc ttttatgggc gaagttaaca
     1081 tctcaccgtt gtcataaagt aaaaatcgtt cgggagaaaa gctcaatacg tcagcttcct
     1141 cagttctgat aaaagcagca taggggacag ggttattttt gttaaccctt tggtacatat
     1201 cccagggatc cccggtgaat tttgtttgaa acggttgcgt gaaattaacc tgataagagc
     1261 gtcctgttct cagggcttgt tgaatggcat tgaatgagtt tctgtaatta tctcttgata
     1321 ttaatggcaa aaattcagat tgaactgaaa attgtaccga ttttgtggat tgattattca
     1381 atatattctt tatttcatct acaacgcttt tggttgaaga atgactgttt cttgcaaata
     1441 aagtggcctt ttttaaatgg tgatcaacta tgatagccca atcataaaac ccgaaattta
     1501 aaacaggtat atgcgccaga tctttttgtg gatatagctc atgatcatgt agtagtgcac
     1561 caaagtcgta tgaaacgtag cctatggctc cgccctgaaa aggaagatca aattctgact
     1621 gctctatttt tagtttattt tttaatttcg ttaatactaa atctttatca tatacattat
     1681 tctcaattac catcctctca tatgggtgag cacttaaaat atcataacgc cccgttgact
     1741 tgtctgtact ctgaagcagg acaaatccag gtaaacgact gagtttttca taggattcag
     1801 atgaaaaatc atggtagtca agtgtaataa gagtactatc aatcaaatta tcacctcaag
     1861 ataaggatgt gctgtgaaaa aatgcaatgc ttttcatttg gtcattttaa cctaacttat
     1921 caataaaaaa cgtatatttt tatcttgggt tcctattaat ttactggttt ttaaattaat
     1981 ttgtgagtat cctatgccaa cgcgttgttg acaaggagcc atgtaatgac aactgttgct
     2041 caatttgaaa taacatacac acaatacctg aatgaacaag gtaagttggt cgctgaatta
     2101 cctccattcg cagataatca atccatactt aaagaattgt ataaaatcat ggtactcacc
     2161 cgtacttttg ataaaaaagc aattgcatta caaagaacag ggaaaatggg aacctatgcg
     2221 ccaattaatg gccaggaagc tatttcgact gcaattggac atgctcttaa acctgaagat
     2281 gtacttgtcc cttattacag agattatgct gctcaatttc agagaggcgt taaaatgtca
     2341 gagatactcg ccttttgggg cggggatgag cgaggaagtc attattctaa taatgccgaa
     2401 gatttgccta tttgtgtccc aattgcatct caatgtttac atgccgcagg agtcgcattt
     2461 gccttcaaat acagaaacca accaagagta gctgtggttt gtattggaga aggaggcact
     2521 tcagaaggtg atttttacga agccatgaat gtggctggag catggaaatt accagtggtc
     2581 ttcgtagtaa ataataacaa atgggcgatt tctgtgccta tagagaaaca aactggctca
     2641 caaacaatag cccaaaaagc gattgctgct ggtttttctg gcgttcaaat agacggtaat
     2701 gatatttttg ctgcaaggca ggttataggg gatgctattg aaaaggctcg tcaaggcggt
     2761 ggacctactc taatcgaagc attaacctat cgcttgtgcg accatacaac ggctgatgat
     2821 gcaacacgct atcagccttc tcatgaagta gaggaggcaa aagccaaaga gcctattgtt
     2881 cgttttaaac actatttaat gcaacaaaat atttggacat ctcaggatga agaaaagctg
     2941 gttatagaat gcagtgaaaa ggtagaaaaa gcagtagagg aatatttgaa taccaaacct
     3001 cagcctgtta gcagcatttt tgattatcat tatgcagaat taccagaata tttaattgaa
     3061 caacgcgcaa tagccatgga ggagtattct catgcctgat attactttag ttgaagctgt
     3121 tactcaggct ttggcgtatg agttagctca tgatgaaaat gtagtagtct ttggcgagga
     3181 tgttggtaaa aatggtggag tatttcgagc tactgtaggt ttacaagata gattcggaga
     3241 aaatagagtt tttgatactc cattagcaga atcgatgata gcaggccttg ctgttggaat
     3301 gtccattcaa ggcttaaaac ctgttgctga atttcaattc atgggtttta tttatccagc
     3361 catgaatcag atcatatctc atgcagcgcg tatgcgtaac agaaccagag gccgcttaca
     3421 ttgccctttg gtttatcgtg ctccgtttgg tggtggaatc agagcaccgg agcatcattc
     3481 tgaaagtaca gaagcattat ttgctcatat tccgggtttg caagtggtta ttccctcttc
     3541 tccaaaacgt gcttatggtc tgttgttggc cgctatacgt aaccctgatc ccgttatctt
     3601 tcttgaacca aaacgaattt atcgcttggt caaacaacct gttccagatg atgggcaagc
     3661 tttgccgtta ggtaagtgtt ttaccctgca acagggagat gatctgacat taattagctg
     3721 gggggccagt atgcatgaaa ccttacaggc agccaaacaa ttaactgatg aaggtatttc
     3781 ctgtgatgtt attgatgtag caacaattaa gcctctggat atagaaacta ttttaagttc
     3841 agtagaaaaa acagggcgtt gcgtcatagt gcatgaagga gcaaaaacct gtggagtggg
     3901 tgctgaaata tcggctcaaa ttatggaaca ttgtatggca gatttactag ctcctgtgca
     3961 aagggttacg gggtatgata cggttatgcc ttatttccaa cttgaaaaac agtacatacc
     4021 tagcattgca cgtattaaaa acactgttat gagtataatg gagtaataat gaatattttt
     4081 aatctacccg atttaggtga aggtttacct gacgctgaaa ttcatgaatg gtttgttaag
     4141 gaaggcgaca cagttaaagc agatcaacct ttagtttcta tggaaacggc aaaagcagtt
     4201 gttgacgtac cctgccctca atcaggcact atcgcaaaac tttatggcaa accaggtgac
     4261 gttatcaaaa caggtgagcc tcttgtagcg tttgtttcaa ctaccgaaaa acctgcagat
     4321 aaaggcacag ttgttggtaa tctggaggaa agtactgatg tgtcagaaga taattttatt
     4381 attggaagtc agcgttcttc tcaccgcgtt aagacaacac ctgctgtaag aatgttagca
     4441 aaaaaattag gagttgattt aagctcttta aaaggtagtg gtgacaatgg tgttattact
     4501 cgagaagatg ttcaaaatca ggccaatatc aattcacaac cacctgccgg gtttgagccc
     4561 ttgcgaggtg ttaggcgagc catgcttaat agcatggtac aatctcatgc agaaatagtt
     4621 ccagtcagta tttttgatga agcagatatc catatttgga aacccaatac agacattact
     4681 gttcgtttaa tccgggctat tatccatgcg tcaacaaagg aacctgctct taatgcctgg
     4741 tttgatacca aacatgccgc acgtagatgt tttaaagaag tgcatcttgg cattgccatg
     4801 gacaatgagg aaggtttatt tgttcctgtt atccatgatg cagaaaaata ttccgacagt
     4861 gaattaagaa aaataattga tgaattcaaa gaatcagttc gtaatcgggc ggttagtgca
     4921 gacaagctaa aaggggcgac aattacgctg tctaattttg gaaaattttc aggacgtttt
     4981 gccagtccaa taattgttcc tccaatggta gcgattttag cggttggaag actttataaa
     5041 gctgccgtaa ttaatgatga ggataaaatt gaagcccaca aaatgcttcc tttatccttg
     5101 agctttgatc atagagccat taccggaggc gaggccactc gtttcttagg tgccgttatt
     5161 gaatctttgc aacaagcatg attttaattt atgttgggct tcgtaaataa cgaggcctaa
     5221 caacaaacaa tattactaca actatctgtc ttttctatat aaaattaact gcgaaaaatt
     5281 tcatcgtggt gatattttaa atttccatct tgagattcaa tccataagca agtagagttg
     5341 atattttgtt aactggcaag gaagagaatg gccatgagaa tttttgtcat gattttgctt
     5401 ttctttttta gcatttcccc aactttgggg gataccactt tgcctgcaat ttttgatgtc
     5461 aaaaatgcaa ataagcaatt tgataaaatc aatctccagt tgtcagtaca aaatctaaat
     5521 ttaaataacc tgaataaggc agtagaaact ctcagtgaac tgtctgcgca atccgatgaa
     5581 tgtatagctg atatacagaa aaaaataaat ggagtggatg tattaatcaa acaagctacc
     5641 tctacaggga atgataaagg agctgattta gtttatctta ataatcaaaa gcatcagtta
     5701 gctgagttac aatctcaatg tcgattattc tcaattcgag ctcaggaagc aatagatgct
     5761 tataaaagtg ctattttgca attaaggcaa gaaaagactt tggcgcgtac cttaccatta
     5821 tggcaattag taagtaaaat tattaattca tctgataata ccaaatggat aaatcacact
     5881 aaagagaaat ttttacttca ggatttgctt gtatatctat ggttttgtat tgttacagga
     5941 agctttttta tctctatcct gatcttgtca aaattac
//
LOCUS       DATNSN010000173         5914 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.173, whole genome shotgun sequence.
ACCESSION   DATNSN010000173 DATNSN010000000
VERSION     DATNSN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.173"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            219..1061
                     /locus_tag="VAH76_002702"
     CDS             219..1061
                     /locus_tag="VAH76_002702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEO1387290.1"
                     /translation="MTKNNTSGKEQEFHIKPFLKWAGGKFQVINKIRSSLPKGSRLIE
                     PFVGSGAVFLNMDYGKFLLADINADLINLFEHLKYEQHDFIYFCKQFFSNEFNKKSTF
                     LSLRSEFNSTKDTHLKAALFLYLNRHAFNGLIRYNSSGKFNTAFGDYKKPYFPENEML
                     AFIQKAEKAEFKCADYSLIMNEATKGDVVYCDPPYVPLSASANFTKYHSTAFGQEDQR
                     RLVDLAKELASKGVPVILSNHDTAFTQNLYQGSSIISFDVQRNISCNGSTRGRARELL
                     ALFD"
     gene            1072..1545
                     /locus_tag="VAH76_002703"
     CDS             1072..1545
                     /locus_tag="VAH76_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEO1387291.1"
                     /translation="MKQGGSYANSSGGVLEGLVEFALTKKGFTVVRYKDWKLNPSSYG
                     EELLLKNVPYEGLYKHASATEFVLMSKAYNLNTRIECKWQQVSGSVDEKLPYLFLNCS
                     EKMMEPHIIILLDGGGAKPGAIEWLRDACEKFNIKEASTSKRKIDLMTHVTHNQF"
     gene            complement(1542..1784)
                     /locus_tag="VAH76_002704"
     CDS             complement(1542..1784)
                     /locus_tag="VAH76_002704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387292.1"
                     /translation="MFYRWNIEEYHKSIKQNASLTRSPARTVKTQSNHIFASIIAYCK
                     LEMLKIKTKLNHFAIKYKLIIRANQIALKELKNMAS"
     gene            1799..2014
                     /locus_tag="VAH76_002705"
     CDS             1799..2014
                     /locus_tag="VAH76_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387293.1"
                     /translation="MTKSSQFPRIGRAALLAIVEWLAKYRDRSNSLEPYGFGSNTETE
                     KQPTNQFIGSDSIEEIQNGYSRKGDNQ"
     gene            2011..4140
                     /locus_tag="VAH76_002706"
     CDS             2011..4140
                     /locus_tag="VAH76_002706"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF019149.1,HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO1387294.1"
                     /translation="MTVLSHNIHQRSQLNAKIQGYHQDRLAIVYIRQSTLQQVERHGE
                     STKLQYGLVEKAHALGWSRDQIIVIDDDLGRSGSNAEGRPGFQRLVAEVGLDRVGLVL
                     GIEMSRLARSCRDWYQLLEVCALFRTLIGDSDGVYDPTSYNDRLLLGLKGTMSEAELH
                     VLKQRMVEGKKAKARRGELGMQLPRGYIKRPSGEIIKDPDEQVQSTISLVFELFDKFS
                     TINAVLAYFVKNKLLMPDRVRIGLEKGELVWRRPNRPTLGNLLHNPIYAGAYVYGRRP
                     TDPRKKKPGRPATGRTVAKPSEWEVLIKDKLPAYITWEQYERNERQLEANSMQGIGVP
                     RQGPSLLSGLLICGRCGLRMSTYYTNNSNKLRYSCTRMSVDYHDPVCQSLVGNPLDQL
                     IEEQILDALKPSALEISLQVAEDIERERKNLLGHWDKQLERARYEVERSYRQYNATEP
                     ENRLVVRTLEKKWEEALSAEEKLKRDYAQFLDKQPAALTASERDAIRQLASDIPALWS
                     AATTTSDERQEIIRLLIERIIVAVEGKTEKVFVEIHWAGGHKTQANLIRPVAKLEQLS
                     YYNELITRAELLRNEGKSFSEIAESLNQEGWKPPKRSPLFKADMVHSLLSRSKVSSNK
                     TTRSQQVNRQLNEWTFRELSQKTNTPEPTLYRWMQKGILKARRDTNTSHGGVWLITAD
                     EIEIARLRSLKDQPRQWIYHSRVTKVH"
     gene            complement(4070..5017)
                     /locus_tag="VAH76_002707"
                     /pseudo
     CDS             complement(4070..5017)
                     /locus_tag="VAH76_002707"
                     /inference="COORDINATES: protein motif:HMM:NF024935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5168..5329
                     /locus_tag="VAH76_002708"
     CDS             5168..5329
                     /locus_tag="VAH76_002708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387295.1"
                     /translation="MLKELNEIDNNRTEHSIIKPFVIGRKTGYSMAMKSVLTQEAFSF
                     SLIETCKQH"
     gene            complement(5496..5729)
                     /locus_tag="VAH76_002709"
     CDS             complement(5496..5729)
                     /locus_tag="VAH76_002709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387296.1"
                     /translation="MKQSLGNVYLILMCGKDGIAVLDWIELKTILDSVHEEVEWIAVS
                     RGKNQMYSLSGSDGNLQYKLGRSDFPSKILKHL"
BASE COUNT         1871 a         1057 c         1180 g         1806 t
ORIGIN      
        1 tgttgcctta caaaaacggt ttttttgcta tatagcataa aaactccttt ttgttatttc
       61 gctaggaaca agcctaggta cttgagctta attgcccagt agtcaagcaa tcactcaatg
      121 ttgaaataac aaaaggcaga gcgccactgt ttaattatag gacatcaatg aatcctaatt
      181 ttctaaacta gactaaaatt agataaaaag gatgaataat gacaaaaaac aatacttcag
      241 ggaaagaaca ggaatttcat attaagccat ttttaaaatg ggctggcggt aagttccagg
      301 taataaataa gattcgaagc agcctaccaa aaggctctcg tttgattgaa ccgtttgtag
      361 gttctggagc agtattttta aatatggact atggcaagtt tcttcttgct gatatcaatg
      421 ctgatctcat taatttgttt gaacatctga agtatgaaca gcatgatttt atttattttt
      481 gtaagcaatt ttttagtaat gaatttaata aaaaatctac tttcctatcg ttgcgttcag
      541 agtttaattc gactaaagac acccacttaa aggctgcatt atttctttac cttaatcgac
      601 atgcttttaa tggcctaatc cgttacaaca gttctggcaa gttcaatacg gcttttggcg
      661 attataaaaa gccttatttt ccagaaaatg aaatgctagc tttcattcaa aaagcagaaa
      721 aggcggagtt taaatgtgct gactatagtt taattatgaa tgaagctact aaaggagatg
      781 tagtttattg cgatccccca tatgtaccgt tatctgcatc ggccaatttt acaaaatatc
      841 attccactgc ctttggtcaa gaagaccaga gaagattagt tgatctagct aaagaattag
      901 cgtccaaagg cgttcctgtc attttatcta atcatgatac ggcttttaca cagaatttat
      961 atcaaggatc aagcatcatc agttttgacg tgcagcgtaa tataagttgc aacggaagca
     1021 caaggggacg ggcacgagaa ttgcttgcct tatttgatta ggggcaatgt tatgaaacaa
     1081 ggtggttcat atgcaaatag tagtggaggg gttctcgaag gactagtcga gtttgctctt
     1141 acaaaaaaag ggtttacagt tgtccgttat aaagattgga aattaaatcc atctagctat
     1201 ggtgaagaac tattattaaa aaacgttcca tatgaaggac tttataaaca cgctagtgct
     1261 acagaatttg ttttaatgtc aaaagcatac aatctaaaca ctaggattga atgtaaatgg
     1321 cagcaagttt ctgggagtgt tgatgaaaag cttccttatt tatttttaaa ttgttctgaa
     1381 aaaatgatgg aaccccacat tattattttg ctagatggag gcggggcaaa gcctggagca
     1441 atagaatggc ttcgtgatgc ttgtgaaaaa tttaatataa aagaagcttc aacttcaaaa
     1501 cgaaagattg atttaatgac tcatgttacg cacaaccagt tttagcttgc catatttttt
     1561 aattccttca atgcgatttg gttagccctg atgatcaatt tatatttaat cgcaaagtga
     1621 ttcagttttg tttttatttt aagcatttcc agtttgcagt atgcgataat tgaggcaaag
     1681 atatggttgg attgagtttt tacagtacga gcaggggatc ttgtaagact cgcattttgt
     1741 tttattgatt tatgatactc ttcgatattc catcgataaa acatctataa acttcaactt
     1801 gacaaaatca agtcaatttc cgagaatcgg gcgtgcagcg ttgttagcca tcgtggaatg
     1861 gctcgctaaa tatcgtgaca ggagtaactc gcttgagccc tatggatttg gatcaaatac
     1921 cgaaacagaa aagcaaccga ctaatcagtt tattgggtca gatagcattg aagaaattca
     1981 aaatggatat agtagaaaag gagataatca atgacagtcc tatcccacaa tatacatcaa
     2041 cggtcccagt tgaatgccaa gatacaaggt taccatcaag atcgtttagc aatagtctac
     2101 ataagacaat cgacattaca gcaggtagag cgacatggtg agtcgactaa gttacaatat
     2161 ggtttagtcg aaaaagcgca cgctttgggt tggtcaagag atcaaattat tgttattgat
     2221 gatgatttgg ggcgatctgg ttcaaatgct gaaggtcgtc caggattcca acgattagtt
     2281 gcagaagttg ggttagatcg cgttgggctt gtgttaggca tagaaatgtc tcgtttagca
     2341 cgctcatgtc gtgattggta tcaattattg gaagtatgtg ctttgtttcg aacactgatc
     2401 ggtgattccg atggggtcta tgatccaacc tcttataatg atcgtttatt actggggcta
     2461 aaaggaacca tgtctgaagc tgaattacac gtgctcaaac aacgaatggt tgaaggaaaa
     2521 aaagcaaagg ctcgtcgagg agagttaggt atgcaacttc caagaggata tataaaacgt
     2581 ccttctggag aaattattaa agatcctgac gaacaagttc aatcaaccat tagccttgtt
     2641 tttgaattgt ttgataaatt ttctacgatc aatgctgtac ttgcttattt tgttaaaaat
     2701 aaactactta tgccagatcg tgtgcgtata gggttagaaa aaggtgaact tgtatggcgt
     2761 cgtcctaatc gccccacatt aggtaattta ttacataatc caatttatgc tggtgcgtat
     2821 gtatacggtc gtaggccgac tgatcctaga aagaaaaaac ctgggcgccc agcaacagga
     2881 cgcacagttg ccaagccttc tgaatgggag gtcttgataa aagataagct tcccgcatat
     2941 attacttggg agcaatatga acgtaacgag agacaactgg aagcaaattc aatgcaaggt
     3001 attggcgttc cgcgtcaggg gccatcttta ttatcaggat tactcatctg tggacgatgc
     3061 ggtttacgca tgtctactta ttacaccaat aatagtaata aattgcgtta tagttgcacc
     3121 agaatgtccg ttgattatca tgatcctgtt tgtcaatcat tagtaggtaa tccattagat
     3181 cagctaatag aagagcagat tttagacgca ttaaagccat ctgctttaga aatcagcttg
     3241 caagttgccg aagatattga gcgagaacga aaaaatcttt taggccattg ggataagcaa
     3301 ttagaacgcg cacgttatga ggttgagcgt tcctatcgac aatacaatgc aactgagcct
     3361 gaaaatcgtc ttgttgtaag aacactagaa aaaaaatggg aagaagcttt atctgctgaa
     3421 gaaaaattaa aacgtgatta tgcgcaattt ttagacaaac aaccagcagc attaactgct
     3481 tctgaacgtg atgccattcg tcaattagca tcggatattc ctgcgctgtg gtccgcagcc
     3541 acaaccactt cagatgagcg ccaagaaata atacgcttat taattgagcg tattattgta
     3601 gctgtagaag gtaagacaga aaaagttttt gtagaaatcc attgggcagg tggtcataaa
     3661 acacaagcaa atttaattcg tcccgttgca aagcttgagc aactcagtta ttacaatgaa
     3721 ctcattacac gtgctgaact acttcgtaat gaaggtaaat catttagtga aattgctgag
     3781 agtttaaatc aagagggctg gaaacctcct aaacgaagcc ctttatttaa agctgatatg
     3841 gttcattctt tgttatctcg tagcaaggtc agtagtaata aaacaacacg ttcacagcaa
     3901 gtaaatcgac aactaaacga gtggactttt agagagctgt cacaaaaaac gaatacccca
     3961 gagcccactt tatataggtg gatgcaaaaa ggaatactaa aagcacgacg tgatacaaat
     4021 acatcgcacg gcggcgtttg gttgattaca gcggatgaaa ttgaaattgc taggctgcga
     4081 tctttaaagg atcaacccag gcagtggatt tatcattctc gagtaacgaa ggttcattaa
     4141 ttttcatttg acttttacgg aggtagtatg actggtagat ctcaattcgc caccgtttct
     4201 ggtagacttc ataaagacgt tcggcactgc tagtcatgtc attagaaacg agatacagga
     4261 tgcctgttga gccgttttcg tttttgaaaa cctttttcag gagcctcact gggaagtcta
     4321 ggcccttaag ccagactgtg tgtgctatat cctcttcatg ctctaatgct cttacttgtt
     4381 ggtaccgccc gttgttggca tcgttttcag ataaagctaa tgtacgatta gatttaatac
     4441 cgatgataaa cgatttgtga aggtcattat ggatatgagc catattggcc ttcgagccat
     4501 accagttatc agccaggata taatcaaaca gcacgttatt tttaactgct tggtcaatga
     4561 gacttcgaaa caattcattc ttggtggtgg atgattttct tcgttcttgc tttgttttga
     4621 tatcactgta ggaaatatcc tttttgataa cctcataacc cacagggaca ctaaaatcat
     4681 cgtatcgaac catacaggtt aggatgttga tacccttcaa aacaacacct tttgcatgag
     4741 aataatgcca acagttgatg tcattctcat ccatgtaagg cttttcttca attgagtcat
     4801 caagcagcag aacaccacca gttgcttctt tttctcgaac aggttttttt acatagcccc
     4861 agagcgactt ggagtcataa tcttcttgtc gaagaaaacg tgtcaccttg tcgtgagcaa
     4921 attcaccatc caatagctct gataaaccag tcgctgttgc atatttgttc tggcatatca
     4981 aataatccgt atacaaatca agcatatcca ttcgtctttc tccctaaaat tcagggcgaa
     5041 attttattca tattttaatg gactgcaaga aggtgtgcgt aacatgagtt aatgagtatg
     5101 acggagtttg tgcaatgggt taattcagtt tttaaataat taaatcgtag ctcatgatgg
     5161 agttattatg ttgaaagagt taaatgaaat tgacaataat cgtactgaac attcgattat
     5221 taaacccttt gttattggcc gcaaaactgg ctattccatg gcaatgaagt cggtgctcac
     5281 acaggaagca ttctccttct cactgattga aacctgtaaa cagcactagg tagatgcctt
     5341 gcatggcttt aaatgtgcaa taaaatatcc ataccgctca aattcttgaa caattagaag
     5401 aactccttcc gtttaacgta aatccctcca aattaatgac atgcgtaata tacctaaact
     5461 tgatttttcc taacaaggag gcgttagtta gccagttaca gatgtttcag tatcttggat
     5521 ggaaaatcac ttctccctaa tttatattgc agattaccat ctgacccaga tagagaatac
     5581 atttgatttt ttccacgtga taccgcaatc cactctacct cctcatgtac tgagtcaaga
     5641 atagtcttca gttcaatcca atccaataca gcaattccat ctttaccaca catcaagatt
     5701 aaataaacat ttccgagaga ttgtttcatc tctaaaagag tgtcttggtg agattttaat
     5761 aaagtaaagc gccaaggaga aaatcttttt gtacaatatt taatatagat gcctatttta
     5821 tcattgataa tataggctga attgttagag tgagagtatg ttttgaattg taaactactg
     5881 gacgactctt gaattagttg cgtaaaaaca attc
//
LOCUS       DATNSN010000174         5897 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.174, whole genome shotgun sequence.
ACCESSION   DATNSN010000174 DATNSN010000000
VERSION     DATNSN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.174"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..650
                     /locus_tag="VAH76_002710"
     CDS             <1..650
                     /locus_tag="VAH76_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387297.1"
                     /translation="PLLQLPTVYVQNGNVIFDKPMPYLIRNDKNQVVIIIDTTGKVAD
                     FTEEYPYLNILIKKNSINFLLPTPELLGSSPVNEQKGVPIVQPLDKDMNVVFDGKKII
                     QDNAINGLKYASQLMIYPIVVAILYSIMVTVLLVIAFLGQVFSKIFFSFTISFKKSSR
                     LLMVASTPMLVVLVTMLSLHFIFPGFGVILLILLAAYYSYGVYGLRDESRKMVLK"
     gene            647..1432
                     /locus_tag="VAH76_002711"
     CDS             647..1432
                     /locus_tag="VAH76_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1387298.1"
                     /translation="MRELIHFAHGNGFPSLCYKQLLGQLETRFDCCFIDKIGHDPDYP
                     VGENWHNLVAEIIASIKRQAEQPVIAVGHSLGGVLSLLAAIEQPKLFKAVIMLDSPLI
                     GAFKSSMVRLAKALGIIDRVTPAFRTKKRREHWQNYEQLISYLKTRNLFKTFTEECLN
                     DYIKYGLQYKEDGYYLRFDRHVEYQIYRTIPHVIPRYKGKLIVPAALIYGDKSTVVDR
                     IDIRYMKNHFNVKCFKTMGTHLFPMEHPKAVGKQVIEVIDAII"
     gene            1525..4011
                     /locus_tag="VAH76_002712"
     CDS             1525..4011
                     /locus_tag="VAH76_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEO1387299.1"
                     /translation="MSELILILAAIGVAGTLLTRHASISVWAISYTIFLGLVLQFSSP
                     GLLSQIVLWAIEAVLVLGSIKPLRREVLSKHLFQIIKKTMPSMSITEREALEAGTVSW
                     EGDLFSGNPNFSLLRNAPTVALTEEEQAFIDGPVSALCEMLDDWDITHNRTDLPPEVW
                     QFIKENRFLGMIIPKQYGGLGFSATAQMSILVKIYGRSITAATTISVPNSLGPGELLL
                     KYGTEEQKNYYLPRLADGREIPCFALTGPNAGSDAASIPDKGVVCRQEFNGELILGVR
                     LNWDKRYITLCPVATVIGLAFRMFDPDNLLGKGEDIGITCALIPATTPGITKGRRHFP
                     LNTGFMNGPTQGKDVFIPLDYLIGGASMAGQGWRMLMECLSAGRAISLPSSASGGAQA
                     VALASGAYARIRKQFNQPIGKFEGIEEPLARIASAAYIIDSSLTMAAAAIDNGAKPSV
                     AGAILKYHTTERARQLALDGMDIHGGKGICLGPNNYIGRGYQGSPIGITVEGANILTR
                     SLIIFGQGAIRCHPYVFKELESIRNNDLVAFDDSFFAHVGFVITNFTKSLIFGWTDAR
                     FSKSPASSVKRYYQLIHKYSSNLAFLADFSMIVLGGELKRKEKLSARLGDMLSCLYMA
                     SAVLKRFYEDGEPRADLPVVHWSCQQLLHECETAMHGVIINFPARWARIILSLTLKPL
                     GNRRHKPDDKLGHKVARILIEPNETRNRITRLVYKKPGDNCPLGRMEEAFHKICAVEE
                     LERKVMKAVKDNILHSLTFQEQILEALEHQILTPVEAKQLEEAERARQDVIRVDDFAD
                     EELRRPSSLGEKVKSEKKIPARDDMETETA"
     gene            complement(4326..4595)
                     /gene="minE"
                     /locus_tag="VAH76_002713"
     CDS             complement(4326..4595)
                     /gene="minE"
                     /locus_tag="VAH76_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEO1387300.1"
                     /translation="MSIFNYLRRRASTASVAKERLQIIISHERSQRNTPDYLPKLQEE
                     ILAVIAKYVNISRDQVSVNLERMEDSAVLELNITMPDKALEDSNS"
     gene            complement(4592..5422)
                     /gene="minD"
                     /locus_tag="VAH76_002714"
     CDS             complement(4592..5422)
                     /gene="minD"
                     /locus_tag="VAH76_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEO1387301.1"
                     /translation="MAKIIVITSGKGGVGKTTSSAAISSGLALLGHKTVVIDFDIGLR
                     NLDIIMGCERRVVYDFINVINGEANLNQALIKDKRVPNLCILPASQTRDKDALTLEGV
                     EKTLNELAKDFDFIICDSPAGIETGALMAMYFADHAIVVTNPEVSSVRDSDRILGILA
                     SKTKRAIENKAPVQEHLLLTRYDPERVERGEMLSVTDVKEILAIPLIGVIPESKSVLK
                     ASNTGTPVILDESSDAGIAYQDAIARFLGEERPMRFISIDKKGLFRRLFSKNKEEVTI
                     "
     gene            complement(5533..5877)
                     /locus_tag="VAH76_002715"
     CDS             complement(5533..5877)
                     /locus_tag="VAH76_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEO1387302.1"
                     /translation="MDQLQRAGSSIAQNIAEGSGEYARYEKHRFYRMAKRSATECAVV
                     LDSARKLSLIQTKKISDARNLLLGILSMLIKMVKSVLKTRAETTISLGTPTYWFCSIL
                     SSTEYKCITTIH"
BASE COUNT         1756 a         1027 c         1223 g         1891 t
ORIGIN      
        1 aacccttgct gcaattgcca actgtttatg tacaaaatgg taatgttatt tttgataaac
       61 caatgcccta tttaatcaga aatgataaaa atcaagtggt aataattata gacacaacag
      121 gtaaagtggc tgatttcaca gaggagtatc cttaccttaa tattctgatt aaaaaaaata
      181 gtattaattt cctattgcca acaccagaat tattaggtag cagcccagta aatgaacaga
      241 aaggagttcc tatcgttcag ccattagata aagacatgaa tgtggtattt gatggcaaaa
      301 aaattattca ggataatgca attaatggtt tgaaatatgc ctctcaattg atgatatatc
      361 caatagtggt tgctatttta tattcgatta tggtgactgt tttattggtc attgcgtttt
      421 taggacaagt attttccaaa atcttttttt cgttcactat atcctttaaa aaatccagcc
      481 ggctgttaat ggtcgcatca acgccaatgt tagtagtttt ggtaaccatg ttatcacttc
      541 attttatttt tccaggattt ggagtgattt tgttgatatt attggcagcc tattacagtt
      601 acggtgtcta tggtctccga gatgaaagca ggaaaatggt gctcaaatga gagagttaat
      661 ccattttgct catggcaatg gatttccatc attatgctac aaacagttat tagggcagct
      721 ggaaactcgt tttgactgtt gttttataga taaaattggt catgatcctg attatcctgt
      781 cggagaaaac tggcataacc tggttgccga gatcattgca agcatcaaac gacaagcaga
      841 gcagccagtg attgcagttg gtcattcttt aggaggggtt ttgagtttgc tcgctgcgat
      901 agagcaaccg aaattattta aagcagtcat tatgctagat tcgcctctca ttggtgcttt
      961 taaatcaagc atggtgagat tggctaaggc tttaggtatc atagacagag taacccctgc
     1021 ttttagaaca aaaaaaaggc gtgagcattg gcaaaactat gaacaattaa tttcctattt
     1081 aaaaaccagg aatttattta aaacatttac agaagaatgc ttgaatgact atattaaata
     1141 tggtttgcaa tataaagagg acgggtacta tctgcgtttt gacaggcatg ttgaatatca
     1201 aatatataga acaattccgc atgttattcc tcgctataaa ggtaaactta tagttcccgc
     1261 cgcattgatc tatggtgaca agagtactgt agttgataga atagacattc gatatatgaa
     1321 aaatcatttt aatgtaaaat gttttaaaac tatgggtaca catttatttc caatggagca
     1381 ccctaaagct gttggaaagc aagttataga agttattgat gctataattt gattagacgt
     1441 cttaactggt ttgcagattt tgttatcgag aaagaagtct attcaattct ttcatttgga
     1501 ttagcctata ataaggagaa tgttgtgtca gaacttatat taatactagc agcaattggt
     1561 gtggctggaa cgttattaac ccgacatgca tcgatatctg tatgggctat tagttatacc
     1621 atttttcttg gtttggtttt gcaattcagc tctcctggct tgttgtcgca aatcgtctta
     1681 tgggcaatcg aagctgtact ggttttaggc tccattaagc ctttaagaag agaagtacta
     1741 tcgaaacatt tgtttcaaat aattaaaaaa acgatgcctt ccatgtctat tactgagaga
     1801 gaggcattgg aagcaggtac agtgagttgg gagggagatt tatttagtgg taatcctaat
     1861 ttctctcttt tgaggaatgc tccaactgtt gcattaactg aagaagagca ggcttttata
     1921 gacggccctg tcagtgccct ctgtgaaatg ttagatgatt gggatatcac tcataataga
     1981 acagatcttc cccccgaagt ttggcaattt ataaaagaga atcgttttct gggaatgata
     2041 attccaaagc agtatggtgg actaggtttt tccgctactg ctcagatgtc aattttggta
     2101 aaaatctatg gccgttctat tactgctgct acaactattt cagtacctaa ttcactgggg
     2161 cctggtgaat tacttttaaa gtatgggaca gaagaacaaa aaaattatta tttaccaaga
     2221 ttggccgatg ggagggagat cccatgcttt gccttgacag gacctaatgc tggttcggat
     2281 gcggcctcta tacctgataa aggtgttgtt tgccggcagg agttcaatgg tgagctcata
     2341 ttgggagttc gtttgaattg ggacaaacga tatattacct tgtgtccggt ggcgacagtt
     2401 attggtttgg cttttagaat gtttgatccg gacaacttgc taggtaaagg tgaagacatc
     2461 ggtattactt gtgcgcttat tcctgcaact actccgggga tcacaaaagg gcgcaggcac
     2521 ttccctttaa atacagggtt tatgaatggt ccaacgcaag gcaaagatgt ttttattccg
     2581 cttgattatt tgatcggtgg tgcttctatg gctggtcaag ggtggcgaat gttaatggag
     2641 tgtttaagcg cgggtcgtgc aatctctttg ccatcaagcg ccagtggagg tgcacaagcc
     2701 gttgcattag catctggcgc ctatgccaga atccgtaaac aattcaatca accaatcggc
     2761 aagttcgagg ggatagaaga gcctcttgcc agaatagcct cagctgctta tatcattgat
     2821 tcttctttaa ctatggcagc agcggctata gataatggag caaaaccttc tgtcgcaggg
     2881 gctattttaa agtatcatac tactgaaaga gcgagacaac ttgcactgga tggtatggat
     2941 atacatggtg gtaaagggat atgccttggt ccaaataatt atattggaag aggatatcaa
     3001 ggttctccta ttggcattac ggtggaaggg gccaatattt taaccagaag cttaattata
     3061 ttcgggcaag gagcgatacg ttgtcatcct tatgttttca aggagttaga aagtattaga
     3121 aataatgatt tagttgcgtt tgatgattcc ttttttgccc atgtgggttt tgttattact
     3181 aattttacaa agtcacttat ttttggatgg accgatgctc gttttagcaa gtctccagcc
     3241 agttctgtaa aaaggtatta tcaattaatt cataagtaca gttccaatct ggctttttta
     3301 gcagattttt caatgattgt attaggtggt gagttaaagc gaaaagaaaa attatcggca
     3361 cgcttaggtg acatgctgag ttgtttatac atggcatctg ctgttttaaa acgattttac
     3421 gaagacgggg aacctcgtgc tgatttacct gttgttcatt ggagttgtca gcagttattg
     3481 catgagtgtg aaacagctat gcatggggta atcattaatt ttccggcacg ctgggcaaga
     3541 attattttat cgttaactct aaaacctctt ggcaacagaa ggcataaacc ggatgataaa
     3601 ttgggtcata aggtagcaag aatactgata gagcctaatg aaacaagaaa tcgaatcacg
     3661 agattggttt ataagaagcc aggggataat tgtcccttag gaaggatgga ggaagcattt
     3721 cataagattt gtgcagttga agagctagag cgcaaagtga tgaaggctgt taaagacaat
     3781 atccttcatt cattgacttt ccaggagcaa atattagaag cacttgagca tcaaatattg
     3841 actccagttg aagcgaaaca actagaggag gctgagaggg ccagacagga tgttataagg
     3901 gtagacgatt ttgctgatga agaactacgt cgcccctcgt cacttggtga gaaggtaaag
     3961 tcagaaaaaa agattccagc cagggatgac atggaaactg aaacagctta atttatacta
     4021 gattcttttc tataatgctg aatagagtta catgatgtaa ttctattcag caattagagt
     4081 caggctaatc attcccatat gtttttatca attttggttt taatcaattg atctttacag
     4141 acagagctat taagaatgag tttaactccc atgcatgtaa tgcttaactc atttaggtat
     4201 tttatttaaa gttttttgaa atattcttga aggagataat gttttaatgc ttatgttaag
     4261 taggcaggta aactacatta cttgatagtg taataatacc tataagtata aaaaacttaa
     4321 tagaattatg agttgctgtc ttccaatgct ttatctggca ttgtaatgtt tagctcaaga
     4381 acggcactat cttccatacg ctccagatta acactcactt gatctcggct tatattcacg
     4441 tattttgcta taaccgcaag aatttcttct tgcagcttag gcaaatagtc tggtgtattg
     4501 cgttgagaac gctcatggga aatgatgatt tgtaagcgct ctttagcaac agaagcggta
     4561 gaggctcttc tgcgtaagta attgaagata ctcatattgt cacttcctcc ttgtttttac
     4621 tgaataagcg acgaaatagt ccttttttat caatacttat aaatcgcatt ggtctttctt
     4681 cgcccaaaaa gcgtgctatg gcgtcttgat aagcaatgcc cgcatcgctg gattcatcca
     4741 gaataactgg cgtaccagta ttggatgctt taagaaccga tttggactca ggtataacac
     4801 cgattaaagg aatagctaat atttccttta catcagttac agacaacatt tcacctcgtt
     4861 ctactcgctc aggatcatat cgtgtcaata acaagtgttc ttgaacaggt gctttatttt
     4921 ctatagctct ttttgttttg ctggccagta ttcccagaat tctatctgaa tctctaacag
     4981 aagagacttc aggattagta acaacaatgg catgatcagc aaaatacatt gccataagtg
     5041 ctccagtttc aattccagca ggggaatcgc aaataataaa atcaaaatct tttgctagct
     5101 cgttgagagt tttttctact ccctccagag ttagtgcgtc tttatcacga gtttgcgatg
     5161 cgggcagtat gcaaagatta gggacccgtt tgtctttgat gagagcttgg tttaagttgg
     5221 cttcaccatt gatgacgttg atgaagtcat aaactacacg tctttcacag cccataatga
     5281 tatcaaggtt tcttaaaccg atatcaaaat caataacaac agttttatgc cctaagagcg
     5341 caagtcccga agaaattgct gctgatgaag tggttttacc tactccgcct ttcccagatg
     5401 taattactat tattttagcc aacgctgaac ccttcctatt tttttcgatt caacacaata
     5461 tttaaccaac agtttacacg ttattcattc attaattcaa gttatctgat gcttaattgt
     5521 tataaaaaca aattaatgga ttgtagtgat acatttatac tctgtactgg acaaaatact
     5581 acagaaccaa tacgtcggcg ttcccaatga aattgttgtc tctgcccggg ttttaagcac
     5641 cgatttcacc atttttatga gcatggaaag aattccgagt aacagatttc ttgcatcact
     5701 aattttttta gtttgaataa gacttaattt ccgggcacta tctaatacta cagcacattc
     5761 agtcgcagat cgtttagcca tgcgataaaa gcgatgtttt tcataacgcg catattcacc
     5821 agagccttct gcaatgtttt gcgctattga tgagcctgct cgttgcaatt gatccactag
     5881 ataacctcaa cctctgg
//
LOCUS       DATNSN010000175         5894 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.175, whole genome shotgun sequence.
ACCESSION   DATNSN010000175 DATNSN010000000
VERSION     DATNSN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.175"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            8..235
                     /locus_tag="VAH76_002716"
     CDS             8..235
                     /locus_tag="VAH76_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1387303.1"
                     /translation="MPTHEIANLVHYYRKQSGLSQQELAHLAGVGKTVIYDIEKGKES
                     VRLNTLLKVLDVLNIQMKFETPFPQTTDNNS"
     gene            232..540
                     /locus_tag="VAH76_002717"
     CDS             232..540
                     /locus_tag="VAH76_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEO1387304.1"
                     /translation="MRKAYVSVNGIKAGILEELQSGKYQFSYFDDYHGAPVSLTMPLT
                     NKVYDFDVFPPFFEGLLPEGIMIEALLRKYKIDKNDYFGQLIIVGQDVVGAVTIEEIR
                     "
     gene            537..1475
                     /locus_tag="VAH76_002718"
     CDS             537..1475
                     /locus_tag="VAH76_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEO1387305.1"
                     /translation="MKRCPITYEKISDQENYSQRGLRLLSPQLKNLSPLDLSADEQRQ
                     EAIARVGKTSIQGVQKKLSAKLKIKEGCFEIVDQNGHYILKPQSDIYPELPENEAITM
                     TLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
                     SMEKVIAIIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITRDRKISISPAY
                     DLLNSTIAQKNTKEEIALLLKGKKNNLTKSDFFNYFAVEKLGLNQNVINGIAQEFHQA
                     IPEWRELISFSFLSQPMQEKYLQLLDQRCKRINFFD"
     gene            complement(1638..2348)
                     /locus_tag="VAH76_002719"
     CDS             complement(1638..2348)
                     /locus_tag="VAH76_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1387306.1"
                     /translation="MLKRFLSSGVVLGLFMSTAFANMVTNGEQFGFIGGNLGLNSVIN
                     KSTETLNWTGGPGSSNTRYAEQGVAGALVIGYVKRFQRVAIGLNGGITYLNNEARSGI
                     YGSFAGSPYSEGDSVKLNHFYEFMFQAGYLLRETSQLYVSAGPAYGKFHFLQSYQFTT
                     HSQPTSSDNAKSLWGINVGIGLKERLSEHLALGVAIQHINFQSQTLQEVHGNNMDHIR
                     YNPSVQIGKIGIEYEFAL"
     gene            complement(2831..3124)
                     /locus_tag="VAH76_002720"
     CDS             complement(2831..3124)
                     /locus_tag="VAH76_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387307.1"
                     /translation="MKKNSSTKKILSSLSNTELQEKQELMMREQDEQLDILLKTVTRT
                     YEIAEAIQEELTSQNKLIDGLNEHVEKTDGKVENTTKKVEALIPEVRSSCFLM"
     gene            complement(3152..4051)
                     /locus_tag="VAH76_002721"
     CDS             complement(3152..4051)
                     /locus_tag="VAH76_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387308.1"
                     /translation="MFSPLQDPWSELEELGFNPEYLQSLKERAATSIEMTKNIEMLAD
                     YYEQFKEVGFTDRDLARIVSLVSFTADNRLANIGSRMMITLVCGGLNLVKAPLHFKPH
                     KLTNMMLSLNGLEKLRTLVEMSKESGFTPFKNQAEFKASFEKRCKKFPMLRVLWCYSD
                     ANESYMEYRKQDAIYKDWLSEEELHQLNRLPRISKTRDLHYWKRDTVRPVLLGDNYYS
                     REDFGQTREEFEKAQDNPDAVESWCDSWANPFDQNPAPALTQSQEKSNQRNASKIPFF
                     TVYENNGKSGVVDEQQSSNAFSN"
     gene            4215..5669
                     /locus_tag="VAH76_002722"
     CDS             4215..5669
                     /locus_tag="VAH76_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symporter"
                     /protein_id="HEO1387309.1"
                     /translation="MENKTKALNFLNRIERCLVPQSNPQIMKQLLSALGLDKLTTGNE
                     EYKLFIQTIKKKSIDLNPLISKVKSDILNNDPLWTLLAYIEENELIDDAEIENASSTI
                     QLQVNLLCLLEAITITMANSNDFAHDIYNHMMKQRGKGATGNPFYNFLFGIPSRATLF
                     ERLKLISIDPGLTSIIFHRLMGANPETQADLDNFVSQNRLSGWNADIELANFDVASTS
                     TLPAMGVNILEAVWEDATGHNKNTGGILNAQAGLGLIGVMEEQEYTTSFKLADTTLPQ
                     GTTLHSDLGYSLMPELKVDNSPKKVSQFHIDKQWMNLYNSWNLLFVIGNIDSVFVPLK
                     LLMPSVFSAEPSNYKETRVLTLFLVANLFLNQQTLNNPLFSNNFSFRNGAAIMKKWGE
                     INKNHASELLQQCCPEKCKELDDLYNHVFGKNPHLNFGLKLFSYFQDSHQFRLTKKAQ
                     PSTSSDYSFFSGNTPSENNSEKMGVALHSSLYTK"
BASE COUNT         1928 a         1110 c          985 g         1871 t
ORIGIN      
        1 gtgttccatg cccacacacg agattgccaa tttagtccat tactaccgta aacaaagcgg
       61 cttatcacaa caagagttag cccacttagc gggtgtcggc aagacagtta tatatgatat
      121 tgaaaaaggg aaagaatcgg tgcggttgaa caccttgcta aaagtattag atgttttgaa
      181 tatccaaatg aaatttgaaa cgccttttcc tcaaacaacg gataataatt catgagaaaa
      241 gcatacgtat cagtgaatgg catcaaggct ggaatattag aagaattaca aagcggaaaa
      301 tatcaattca gttactttga tgattatcac ggggcacctg tttctctcac aatgccgtta
      361 accaataagg tctatgattt tgatgtattt cccccttttt ttgaagggtt actccctgaa
      421 ggcattatga ttgaagcact attacgcaaa tataaaatcg ataaaaatga ttattttggc
      481 caattgataa tagttggcca ggatgtcgta ggcgcagtga ctattgagga aataagatga
      541 aacgctgccc cattacttat gaaaaaatca gtgaccaaga aaactattca caacgtggat
      601 tgcgcttact ttctcctcaa ttaaaaaacc tgagcccatt agatttaagt gctgatgagc
      661 agcgtcagga agcaattgct cgagtaggaa agacgtcaat tcaaggtgtc caaaagaaac
      721 taagcgcaaa actaaagatt aaagagggat gttttgaaat cgtggatcaa aatggtcact
      781 atattttaaa accacaaagt gatatttatc cagaattgcc ggaaaacgaa gccattacca
      841 tgacccttgc aaagaccatt ggacttgaag tgcctgttca tggtttagtg tactctaaag
      901 ataacagttt gacttacttc attaaacggt ttgatagaat aggccataat aaaaaattag
      961 ctttagaaga ttttgcacag ctatcgggtg aagatcgaca tacaaaatat aaaagttcta
     1021 tggaaaaagt gattgcaatc atagagcagt tttgcacgtt ccccaaaatt gaattcgtga
     1081 aattatttaa gttgacattg tttaacttcc tggtcggcaa tgaagacatg cacctaaaaa
     1141 atttttctct aattaccagg gatagaaaaa tttccatatc accagcatac gatttactta
     1201 actcaactat cgctcaaaaa aacacaaaag aagaaattgc attgctcctc aaagggaaaa
     1261 aaaataattt aacaaagagt gattttttta actattttgc cgttgaaaaa ttaggattaa
     1321 atcagaacgt catcaatggg atcgcacaag agtttcatca ggcaatacca gaatggcgag
     1381 agttgattag tttcagtttt ttatctcaac cgatgcagga aaaatatctc caattgctcg
     1441 atcaacgatg caagagaata aatttttttg attaactgat atcagcggtc acatcattat
     1501 tcattttgtg gtttaaatcg cttccaggca taagccggta aattcaaact atcctcaaca
     1561 gtagcaatat ttaaaataaa taattaacgt tgattgcctg ttaattgccc atctaatttt
     1621 ctgcattgga acactatcta caatgcaaat tcatattcaa tgccaatttt cccaatctgt
     1681 acggaaggat tataacgaat atggtccata ttatttccat gaacttcttg taaagtctgt
     1741 gactgaaaat taatatgctg tattgcaaca ccaagcgcca aatgctcaga aagtctttct
     1801 tttaaaccaa tacctacatt aataccccat aatgactttg cattatctga gctggtaggt
     1861 tgagaatggg tagtaaattg ataggattgt aaaaaatgaa actttccata agcaggacca
     1921 gcactgacat acaattgtga agtttctctt agtaaatagc ctgcttgaaa catgaattca
     1981 taaaaatgat tcaatttgac actatcccct tcgctataag gagatcctgc aaatgagcca
     2041 tatattccgg aacgtgcctc attattgaga taggtaatgc ccccgtttaa cccgattgcc
     2101 actctttgga atctcttgac ataaccgatg actaaagcgc ccgctacccc ttgctctgca
     2161 tatctggtat tagaacttcc aggtcccccc gtccaattaa gggtttcggt agatttattt
     2221 attacagaat taagaccaag attgcctcct ataaatccaa attgctcgcc atttgtaacc
     2281 atgttcgcaa aggcagtgct cataaatagg cctaacacca cgccggaaga taaaaacctc
     2341 tttaacatca ttcactcctt tgattaatac aattaaaatt ccagaataga aattaaagaa
     2401 tatcttcaac gttcagttca aaagacttga ttttgataca gttgaaaaaa aaatacaatt
     2461 caactccccc agtttgagct agttttattc ccaagcagct ttatagaagc aactattatt
     2521 tttcatgatt gctaaacaac agaaaatagt agtggtgttc atgcacatta ttcagaattg
     2581 ctcactatcc ccaaaaactg aagagaaaaa tatggctcta tcattacaat cctgtaaaac
     2641 tgcctaatgc aaaaacagat ggttttttag gctttgttta aaagagacgg ttagtgtaac
     2701 ctggaaggat atttttcttt tacacaatga aaacccggga tacggactac ccctctccca
     2761 ggctacttac cgatcatacg acgcaaggct tttcttcttt ggggctgcac ccacagtcaa
     2821 ttcgccagga ttacatcaag aaacagcttg accttacttc agggataaga gcttccactt
     2881 tttttgtcgt attttctact ttaccatcag tcttttccac atgttcattt agtccatcga
     2941 ttaatttatt ttgactggtc aactcttctt gtatcgcttc agcgatttcg taagttcgag
     3001 ttacagtttt taataaaatg tccaactgtt cgtcttgctc tctcatcatc agctcttgtt
     3061 tttcctgtaa ttccgtatta cttaaagaag ataatatttt tttagtactg ctgtttttct
     3121 tcatgattgc aaccttattt tatttacttt attaatttga aaaggcattt gaagattgtt
     3181 gctcatcgac aacaccactt tttccattat tttcataaac agtaaaaaaa ggtattttac
     3241 tggcgtttct ctgattcgac ttttcttgtg attgtgttaa agcaggagct ggattttgat
     3301 caaaaggatt tgcccatgag tcacaccaag attccactgc atctgggttg tcttgtgctt
     3361 tttcaaactc ttcccgtgtt tgtccaaaat cttctctgga ataatagtta tcgccaagta
     3421 aaactggtct tactgtatcg cgtttccaat agtgaagatc tcgcgtctta gatattcgag
     3481 gtaatctgtt aagttgatga agttcctctt cgcttagcca gtctttatag attgcatctt
     3541 gttttctata ttccatgtag gattcatttg catcagaata acaccataaa accctgagca
     3601 ttggaaattt tttacatctt ttttcaaaac tcgctttgaa ctctgcctgg tttttaaatg
     3661 gtgtgaaccc actttctttg ctcatttcaa ctaatgttcg caatttttcc aaaccattta
     3721 aactaagcat catattagtc agtttgtgcg gtttgaagtg gagaggagct tttaccaagt
     3781 ttaatccccc gcagactaag gttatcatca ttcggcttcc gatgtttgcc agacgattat
     3841 ccgcagtgaa actgaccaaa ctcacaattc ttgccagatc gcgatcagtg aaacctactt
     3901 ctttaaattg ttcataatag tccgccaaca tttctatatt tttagtcatc tctatagaag
     3961 tagcggctcg ttctttaaga ctttgcaggt attccgggtt aaaaccaagc tcttctaatt
     4021 cggaccacgg atcttgtaat ggagaaaaca tataaatcac cttaaatcag ttaaaaaagg
     4081 acaaaaaata tatcacgtga ttatttattt tactatttcc aatttagaaa ccgttatttt
     4141 ccaaatgaat acattctcta aaaaaatcaa tctgtgtaaa atagacacac ttaggcttaa
     4201 tttaggaata taatatggaa aataaaacaa aggcattaaa ttttcttaac cgtattgaac
     4261 gatgtcttgt accccaatca aatcctcaaa taatgaaaca attattatca gctcttgggc
     4321 ttgataagct aaccactggt aatgaagaat ataagctttt tatccaaaca attaaaaaga
     4381 aaagcattga tctgaatccg ttaattagca aggtcaaatc agatatttta aataatgacc
     4441 ctttatggac tttgcttgcc tatatcgaag aaaacgaatt aattgatgac gctgaaatcg
     4501 aaaatgcttc ttctacgata cagcttcaag ttaatttatt atgcctttta gaggcgataa
     4561 ccattacaat ggctaacagt aacgattttg ctcatgatat ttataaccat atgatgaagc
     4621 aaagaggaaa aggcgcaaca ggtaatcctt tttacaattt tttatttgga ataccttctc
     4681 gtgccacctt atttgaaaga ttaaaactga tttccataga tcctggtttg acaagcataa
     4741 tattccaccg actgatggga gcaaatcccg aaactcaagc tgatcttgat aattttgtaa
     4801 gtcaaaatcg tttatccggg tggaatgctg atatagaact tgctaatttt gatgttgcat
     4861 caacaagtac tttacccgct atgggagtta atattttaga agcggtgtgg gaagacgcaa
     4921 caggccacaa taaaaatacc ggtggtattt taaatgcaca agctggcttg ggattaattg
     4981 gcgtcatgga agaacaagaa tacaccactt cttttaaatt agctgacact actttgcccc
     5041 aaggcactac acttcattca gatttaggtt attcattgat gcctgagtta aaagttgata
     5101 actcacctaa aaaagtatct cagtttcata tcgataaaca atggatgaat ttatacaata
     5161 gttggaattt attgtttgtg attggaaata ttgattctgt atttgtacca cttaagttgt
     5221 taatgcccag tgtgttttca gcggaaccat caaactataa agaaacgaga gtgctcacct
     5281 tatttcttgt tgctaatctc ttcttaaatc aacaaactct taataaccct ctgttttcga
     5341 ataatttttc ctttcgaaat ggagctgcca ttatgaaaaa atggggtgaa atcaataaaa
     5401 atcatgccag tgaactcttg caacaatgtt gtccggaaaa atgcaaagag ctggatgatt
     5461 tatataatca cgtattcggt aaaaaccctc acttaaactt tggattaaaa cttttctcat
     5521 attttcagga ttcacatcaa tttagactca ctaaaaaagc acaaccaagt acatccagtg
     5581 attattcatt tttctcaggt aataccccat ctgagaataa ttcagagaaa atgggtgtag
     5641 cccttcacag ttctttatat acaaaataaa atgttatgct ttatacccag caaataaata
     5701 tcagaatcgg agacaaatta tagatggtct ccgtttttga gaaaaaattt ctttctgaaa
     5761 tctttcacga ggatacccga ctatccgctt taactgctgt tttcatggaa taaactaata
     5821 gcgttggtct atgtcagtag gtatgcggtt gatttacaaa actggtatat aagtctatac
     5881 catttaaaaa gaac
//
LOCUS       DATNSN010000176         5888 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.176, whole genome shotgun sequence.
ACCESSION   DATNSN010000176 DATNSN010000000
VERSION     DATNSN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.176"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(300..896)
                     /locus_tag="VAH76_002723"
     CDS             complement(300..896)
                     /locus_tag="VAH76_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387310.1"
                     /translation="MKIRFTLLCFSIFLTGCVSKPPADINNICNIFKQYPKWYVDSKD
                     VERRWKVPVPVQMAIIHQESKFNARARPPRTKLLWIIPWTRPSTAYGYTQALRSTWDN
                     YKRTNGWFWSSRDDFGDGVDFIGWYANQANIKAGIPRTDAYSLYLAYHEGIGGYQRKT
                     YLKKPWLISVARKVKAKSQLYAMQLNSCQGSLKSRSWW"
     gene            1141..1911
                     /locus_tag="VAH76_002724"
     CDS             1141..1911
                     /locus_tag="VAH76_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO1387311.1"
                     /translation="MTILVFDIETIPDIESGRRLFALDGLSDEDTACAMFALRREKAG
                     NDFLPHYLQKICAISVVISHGNQIKVWSLGDENSDEKELITRFFAGIDKHTPTLVSWN
                     GSGFDLPVLHYRSLYHSISAPTYWETGENQQTFKWNNYLSRFHYRHIDLMDVLAGYQN
                     RAFAPLDDISTMLGFPGKMGMSGSKVWEQYQAGQLKEIRDYCETDVLNTYCVYLRFEL
                     MRGTISQEGYDRNIAQLKNYLGAEKDKMHLQEFLKKML"
     gene            complement(1964..3106)
                     /locus_tag="VAH76_002725"
     CDS             complement(1964..3106)
                     /locus_tag="VAH76_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO1387312.1"
                     /translation="MKLYFFGDDNNKPKLSEHLIIYILSLLDPKSQSVASLVNKDWSR
                     LVQLTRQYLSMLPVIHRIPLFANLGLDVLKLKLMTGGMTNSTFRLRIKNAPEKWVMRI
                     PGIGSSAFITRRDEAHNAKQAEKLGLNVPIAFFDPEDGLQVTRFIEGVHSLDDKALAR
                     KEILHEVASMMRRLHKSPPFDNNTLFFERNEELLDLLKRKSFNFPGEIEFIEQQMKNL
                     KEIFSSYNIQQFPCHNDTTPLNFILSENPEIKGSEKIHQIDWEYSSNNDFIWDLVYFM
                     VEAKLNREQQLILLKAYFNTEELSGSLLAWIEVYKPIIEWWITIWSWTQLANGANAVD
                     LSSYEQLGFERYHNTLSHLKSEDYVKALALIEADSHSIIFTGQRPF"
     gene            3205..4590
                     /locus_tag="VAH76_002726"
     CDS             3205..4590
                     /locus_tag="VAH76_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEO1387313.1"
                     /translation="MFLHMNRYISLYFLLILLIAPIHLLGLDSYYYWDWSRHLALSYY
                     DGSPLIAYFIRIATLLFGDTLFALNLVTIVSTALTSFIIYKTARLCLNKEASYVTVLI
                     WLFSPLVTLDLINQTTYDTPLTLFWALTLYFTIRYIQFNRNQDLYYVGLSIGLMLLSK
                     YSGIVLVLALLIFLVATPYRRLFKSRHFYLALTLAIIVFSPVILWNAQHDWVSIRYQL
                     STHQTQNTHYAMGSVLHAFFSIFLPAINFLFIPPALYLSKGTPRKSPVFYLCLIICVT
                     FGLFYLFVAGKATIRGYWLSQYIITASLLAGYCYQEWAYRNSTYLLIVFYALTSGIIL
                     MDNTTLFSFGYSKKLAYYQSIQQINQSNDKLPSIVITPGWFEARMLFFLKNKPTIFTF
                     DCGSMQNQYRYWSPNLNTLGEVLYIDTFDRLSCVKKYFAQCQSLNLPATFDEKGKPII
                     YAYWCSNKLDT"
     gene            complement(4627..5793)
                     /locus_tag="VAH76_002727"
     CDS             complement(4627..5793)
                     /locus_tag="VAH76_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO1387314.1"
                     /translation="MPKEKVVIIIPTYNEEDNISNTIDLILKETYSLEDYQVDILVFD
                     SHSTDSTAHIVRELTYTSQRIHFAQEPQKTGLGSAYLQAMRIAIDELNADVVFEFDAD
                     GSHQPCYIAPMLELLKQHDVVVGSRYISGGRIPSNWAFHRKLLSVGGNWVARMLLTGK
                     YKDLTSGLRAIRTNLLKKLLPSNFLSNNYAYKLELYWLLHKAKARIVEFPIQFIDREK
                     GKSKLPKNSMIDTLRVLFTLRFRELRQYLTMCCVGSIGALIQFSLYNWLRSSNHSALV
                     SSQVAISAAIISNFIFHHSITFKQKTDFRITKTTFSRFTQFIIYSTFMVYFQSLWVHI
                     FVHSFGQGVLKENIIVASGLGIGSIINYLFYSNIIWSSKGTHSINSNSSKETQI"
BASE COUNT         1855 a         1119 c         1029 g         1885 t
ORIGIN      
        1 tggtcatgca tagtcttaaa aaacgaaatg agcagctcaa aaagcagttt ttttaggact
       61 attatctatt ataaggattg agtaggcaca tcataagcag accatcactc accttttctt
      121 ttcaagcttt attctgttaa ataaacagga ttttactcga ttttctattt aaaaatagaa
      181 atgtatacct tacttaagat tgactttgtg ttatggcgat tattataaac acacaaaatc
      241 atatatgttc catgtctgac tttatttcca ctgtcaagaa cataattagg ccatcttaat
      301 taccaccagg aacgtgactt caacgaacct tgacaagaat tgagctgcat tgcataaagt
      361 tgagatttag ccttcacttt gcgcgccaca gaaattaacc atggtttttt caaataggtt
      421 ttcctttgat aaccgcctat cccttcatga taagccaaat aaagtgagta agcatctgtt
      481 cggggaatac ctgccttaat attcgcctgg ttagcatacc atcctataaa atcaacacca
      541 tctccaaaat cgtcgcgtga tgaccaaaac catccattgg tgcgtttata attgtcccaa
      601 gtcgaacgca acgcttgagt atatccgtaa gctgtcgatg gccttgtcca tggaattatc
      661 cataacagtt tggttcttgg tggcctggct cgggcattaa attttgattc ttgatgaata
      721 attgccattt ggaccggaac cggaaccttc cagcgtctct caacatcctt tgaatccaca
      781 taccatttcg gatattgttt aaatatatta caaatattat taatgtctgc tggaggtttg
      841 ctgacgcagc ctgtaagaaa aatgctaaaa caaagtaaag taaatcgaat cttcatgatt
      901 taatgataaa aactcatcct gcagtattga atatcaatgc tacgatataa ggtatcaatt
      961 taaaaaatat tgtatcaaat atactgcata caatctatgc catttaaagt tacttctatt
     1021 ccttcacatg atagaatcaa tcagatatcc caattgtgat tttgacaaat ctgagacttg
     1081 tcgatagttt caagcaaatt tgcaaaaata agaattaaga ccgaactgga tcaacaaatt
     1141 atgacaatac ttgtttttga tattgaaaca attccagata ttgagtcagg gcgaaggctt
     1201 tttgcacttg atggcttatc agatgaagat acggcatgcg ctatgtttgc cttaaggcgt
     1261 gagaaagcag gcaatgattt tttgcctcat tacttacaaa aaatctgcgc tatatcagtg
     1321 gtaatcagcc atggaaatca aataaaagta tggtctcttg gcgatgaaaa ttctgatgaa
     1381 aaagaattaa taacccgttt ttttgctgga attgacaaac atacacccac cctggtgagc
     1441 tggaatggga gcggttttga tcttcctgta ctgcattacc gatcactata ccatagtatt
     1501 agcgcgccca cttattggga gacaggggaa aatcaacaaa cctttaaatg gaacaattat
     1561 ctaagccgtt ttcactaccg acatattgat ctaatggatg ttcttgcagg atatcagaac
     1621 agagcatttg cccctcttga tgacatttca accatgttag gtttccctgg gaaaatggga
     1681 atgagtggct ctaaagtttg ggaacaatac caggcaggcc aattgaaaga aataagagat
     1741 tattgtgaaa cagacgtact taacacctat tgtgtgtatt taagatttga gctgatgcgt
     1801 ggcacaatta gccaggaagg ttatgacaga aacatagcac aactcaaaaa ctatttaggt
     1861 gctgagaaag acaagatgca tttacaggaa tttctcaaaa aaatgctctg aagatcttaa
     1921 tcgtcactgt aaacgagttg tcctctctag ataatggcgg cagctaaaaa ggtctctgcc
     1981 ccgtaaaaat aatgctatga ctatctgctt caatcagtgc cagggctttg acatagtcct
     2041 cacttttcaa gtgacttaag gtgttatgat accgttcaaa ccctagttgc tcataagaac
     2101 tcaaatcaac tgcattagct ccatttgcca attgtgtcca tgaccaaatg gttatccacc
     2161 actcaatgat gggtttataa acctctatcc atgccaacag agagccactc agctcttctg
     2221 tattaaagta agctttcaac agaataagct gttgttcgcg gtttagcttg gcctcaacca
     2281 taaaataaac caaatcccaa ataaaatcat tattacttga atattcccaa tcaatctgat
     2341 gaatcttttc agaacccttg atttctggat tttctgacaa gataaaatta agtggcgttg
     2401 tatcattgtg gcaaggaaat tgctgaatat tataagaaga aaaaatctcc tttagatttt
     2461 tcatttgttg ctcaataaat tctatttccc ctgggaaatt aaaggatttt cgttttaata
     2521 aatccaataa ctcttcattt cgctcaaaaa aaagcgtgtt attatcaaag ggaggagatt
     2581 tatgaagtct tctcatcata gacgctacct catgcagaat ttccttccgg gccaaggctt
     2641 tatcatccag agaatgcact ccttctataa agcgcgttac ctgcagccca tcttctggat
     2701 caaagaaagc tattggaaca ttcagtccaa gtttttcggc ttgtttagca ttgtgagctt
     2761 catctcttct ggttataaat gctgaagagc ctattccagg aatccgcata acccatttct
     2821 caggcgcatt tttgattctt agcctaaatg tggaattcgt cataccacct gtcattaatt
     2881 ttaatttcaa aacgtcaaga cccaaattcg caaataaagg tattcgatga atcaccggta
     2941 acatagacag gtattgccgt gttaattgaa ccagccgaga ccagtcttta ttcactaaag
     3001 aagcaacaga ctgagacttg ggatctagca aactgagtat gtaaatgatt aaatgttcgc
     3061 ttaatttggg tttattatta tcatcaccaa aaaaatataa tttcataaac gtcatttatt
     3121 aattaatgat aaaactgaat gcattatata aaaactgata ataaaataac atacaattca
     3181 tcattacttt ccagggaata ctgaatgttt ttgcacatga atcgctacat tagcctctat
     3241 tttctactca ttctattaat tgcaccaatt cacctccttg gattagatag ttattattac
     3301 tgggattgga gcaggcattt ggctctatca tactatgatg gttctccctt gattgcctac
     3361 tttatccgca tagccacact tttatttgga gatactctct ttgctttaaa cctggttact
     3421 atagtttcta ctgccttgac cagttttata atttataaaa cagcaagatt atgccttaat
     3481 aaggaagcca gttatgttac ggttttgatt tggttattct caccattagt cactttagat
     3541 ttgatcaatc aaactaccta tgacacacct ttaacccttt tttgggcatt aactctttac
     3601 tttacaataa ggtatatcca gtttaatcgt aatcaggatt tgtattacgt aggtttgagt
     3661 ataggcctta tgcttttatc caaatactca ggtattgttc ttgtgctggc cttacttatt
     3721 tttttggttg caacacccta tagacgactt tttaaatcca gacattttta tttggcatta
     3781 accctggcaa tcattgtctt cagcccggtt attctatgga atgcgcagca tgattgggta
     3841 tccattcgct accagttaag tactcaccaa acgcaaaata ctcattatgc gatggggagt
     3901 gtcttgcatg ccttttttag tattttcctc cctgccatta actttttatt tattccaccg
     3961 gcgttatatc tatccaaagg gacgcctaga aaatcccctg tattttattt gtgcctcatt
     4021 atttgcgtaa catttggtct tttctattta tttgtcgccg gcaaagcaac gataagagga
     4081 tattggttga gccaatacat aattactgcc agcttattag ctggttattg ctatcaagag
     4141 tgggcttatc gtaattctac ttatttatta attgtattct acgctcttac cagtggaatc
     4201 attttgatgg acaataccac tcttttcagt tttgggtatt caaaaaaact ggcttattat
     4261 caatcgatac aacaaattaa tcagtctaat gataaactcc caagcatagt cataacccca
     4321 ggatggtttg aggcacgcat gctgttcttt cttaaaaata aaccaacaat ttttaccttc
     4381 gactgcggtt caatgcaaaa tcaatacagg tattggtcac ctaatctgaa tacccttggc
     4441 gaagtgctat atatcgatac atttgataga ttatcatgtg ttaaaaaata ctttgcccag
     4501 tgtcaatcat tgaatttgcc agctactttt gatgaaaaag gtaagccaat catttacgcc
     4561 tattggtgca gtaataagct ggatacttaa atgaaatagg acttataccc ccaatctctt
     4621 ttgctttcaa atttgagttt ctttagaaga gtttgaattg atagaatgag tgccttttga
     4681 tgaccaaata atatttgaat aaaacaggta attgataata gatccaatgc ccaaacctga
     4741 ggctacaatg atattttctt tcaaaacacc ttgtccaaag gaatggacaa aaatatgaac
     4801 ccataggctt tgaaaataaa ccataaatgt ggaataaata ataaactgtg taaaacggct
     4861 aaacgttgtt ttagttatcc ggaagtcggt tttttgtttg aatgtaatgg agtggtgaaa
     4921 aataaaattg gatataatag cagcgctgat agcaacttgg ctggatacca gagcggaatg
     4981 gttcgaactg cgcaaccaat tgtataagct gaattgaatc aatgcaccga tggagcccac
     5041 acagcacata gttaggtact ggcgtaattc tctgaagcga agggtaaaaa gaacgcgcaa
     5101 ggtgtcaatc atactgttct tgggtaattt acttttccct ttttctctat caataaattg
     5161 aataggaaat tcaacgatcc tggcttttgc cttgtgtaaa agccaatata actcaagttt
     5221 atacgcgtaa ttattggata ggaagtttga aggcaatagt ttctttaaca gattggtacg
     5281 tattgctctt aagccagaag taagatcttt atattttcca gttaataaca tacgagcaac
     5341 ccaattacct cccacagata ataatttacg atggaatgcc cagtttgagg gaatccttcc
     5401 ccctgaaata tatcggctcc ccacaactac atcatgctgt ttaagtagct ctaacattgg
     5461 agcaatataa caaggttgat gagaaccatc tgcatcaaac tcaaatacaa catctgcatt
     5521 taactcgtct attgcaattc tcatagcttg caggtaagcg gaaccaagtc cagttttctg
     5581 tggttcttgt gcaaaatgaa tacgttgaga ggtataagtt agctctcgaa ctatatgggc
     5641 tgtagaatct gtggagtgac tatcaaatac aagaatgtca acctgataat cttcgagtga
     5701 gtaagtctcc ttaagaatca aatcaatcgt attagaaata ttatcttctt cattgtaggt
     5761 agggataata ataacgactt tttctttggg catactgatc agatcataag aagcagatta
     5821 tagaagtaag caaatcttat caaatggtgg acttgccgta aacaaacttt aagtggcagt
     5881 tactattg
//
LOCUS       DATNSN010000177         5851 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.177, whole genome shotgun sequence.
ACCESSION   DATNSN010000177 DATNSN010000000
VERSION     DATNSN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.177"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            19..396
                     /locus_tag="VAH76_002728"
     CDS             19..396
                     /locus_tag="VAH76_002728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387315.1"
                     /translation="MIVSSLELHTHPEEIIKKYKSRMSIEEALRDTKSILYGFSFNIN
                     QTMKQERYIVWLLLAALAAVVAWIVGFVAEKNKLHHQFQSNTYRHKRVLSFVFLGCRI
                     INKNIQILFEYDSLSIIFQGEQK"
     gene            541..1089
                     /gene="hslV"
                     /locus_tag="VAH76_002729"
     CDS             541..1089
                     /gene="hslV"
                     /locus_tag="VAH76_002729"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEO1387316.1"
                     /translation="MEQFHGTTILSVRRGNQVVIGGDGQVTLGNTVMKGNARKVRRLY
                     KDKVIAGFAGGTADAFTLFERFEAKLEMHQGHLIRAAVELAKDWRTDRILRRLEAVLA
                     VADSKASLIITGNGDVIEPEESLIAIGSGGPFAQAAARALMENTQLSAKEIVQKALTI
                     AGDICIYTNNNLTIEELNDEGK"
     gene            1091..2416
                     /gene="hslU"
                     /locus_tag="VAH76_002730"
     CDS             1091..2416
                     /gene="hslU"
                     /locus_tag="VAH76_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEO1387317.1"
                     /translation="MVMTPREIVQELDKHIIGQDDAKRAVAIALRNRWRRMKIKDPVL
                     RNEIMPKNILMIGPTGVGKTEIARRLANLAKAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LTDMAIKQEREFAMKKVEHLAEDAAEERILDVLLPPARGTLTPGEKNTTARQVFRKQL
                     REGELNDNEIEIEVAATPVGIEIMAPPGMEEMTSQLQSMFQQVGSYRTKTRKMTVAKA
                     MKILREEEAAKLINEEDIKLKAIESVEQNGIVFIDELDKIAKRSDTVSGGDVSREGVQ
                     RDLLPLVEGTTVSTKYGMVKSDHILFIASGAFHVAKPSDLIAELQGRLPIRVELSALS
                     VEDFVRILTEPSASLTLQYSALMETEGLTLTFDETGIRRIAEVAWQVNERTENIGARR
                     LYTVMERLLEVVSFEATDKAGETVHVDKAYVDKNLGQLIADEDLARYIL"
     gene            2616..4190
                     /gene="wipB"
                     /locus_tag="VAH76_002731"
     CDS             2616..4190
                     /gene="wipB"
                     /locus_tag="VAH76_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipB"
                     /protein_id="HEO1387318.1"
                     /translation="MKTQRIIHPNIDIRKFPEVNADFSMTDISMGDLHANALLFLNIL
                     VRQGIIAISPENYAKFAEIYTLPELQADYWGTEAPVFSAGNKQERLEEIKKQYNALIA
                     QIKIINTKKLIRLIGDELVDRGVIDYFILKLLQALNDQGADFEILLSNHGIEFVEACE
                     LFKENGNKLVAKRLGNIQHGNSFHALQEAIAAGSISNEEVLNIYHQVYKKHLKIISYA
                     LDPDANEIKVFSHAGIGLNHIRGLARKFKVPYSEESAVDLARTIDAINKKFAEKASAG
                     EIHTLYTHDMMYRGYAGEYLNSTDEVVAATVWGREYGDLIRTSKKFKVTFIHGHDSYD
                     PEKVEHVTLNNQLGQFQNNVGDLYLYATNGMRVVPTQCLNPDKKVQSLREKNRPDKPN
                     DYVVKVHHTKPSFFKTAHPKMTFPDSYKRIWDSTPGHSNITKIKALLKDYTKEDNILG
                     SFWGLIFTLHWGRHHVKSVHQIAQTQYTSVEAILSDLKALKPRDGGSLDKRIKFIESQ
                     IITQRSDNPDLQFNLK"
     gene            complement(4339..5577)
                     /locus_tag="VAH76_002732"
     CDS             complement(4339..5577)
                     /locus_tag="VAH76_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEO1387319.1"
                     /translation="MNWKEKVKTKFYSCDRFVRFVIQHFIQDDCTYIASALAFTSLLA
                     VVPLMSVGLAIFSSFPVFQGLAEPVQNFIFDNFVPATGKIVQSYLQQFTSQVSKLSIW
                     GIVFLIFTALLVMFTIERAMNKIWRVSSSRHGVSAFLLYWAIISLAPVLLGLSLAASS
                     YLFSMPILADHRAPYTILHYSPFFLSLIGFTFLYVVVPNCPVKIRHAFWGGLVAAILF
                     ESAKHAFAYYLIRYNTYELLYGAFATVPIFFIWVYWVWIITLLGAEISYAFSVHHQRR
                     GGKSLDGFSHALLWLHQLWIAQQHGKGLSFNDLVDASKQPFAVDVDEMINALIYHELI
                     HATADGHYMLSRDLSHVTLYDLTQLLPYRLPTHLELQYSKASLAEQWRAAFKRHNEEL
                     KKSLDINLEELFKKTGTVLK"
BASE COUNT         1951 a         1012 c         1202 g         1686 t
ORIGIN      
        1 gggtatagag agccatgggt gattgtatcc tcactggaac tccatactca tcctgaagaa
       61 attataaaaa aatacaaatc aagaatgagt atcgaagaag ccttgagaga tactaaaagt
      121 attttatatg gatttagctt caacataaat cagacaatga aacaagagcg ctatatcgtc
      181 tggctattat tagcagcgtt agctgctgta gttgcttgga ttgtcggatt tgttgcagaa
      241 aaaaataagc tgcatcatca atttcaatcc aatacctatc ggcataaaag agtcttgtcc
      301 tttgtattcc ttggctgtcg catcatcaat aaaaatattc aaatcctatt tgaatatgac
      361 tccctgagta ttatttttca gggagagcaa aaatgagtcg atacctcagc cgtaagggag
      421 tggacatgac gattaatatt ttcagatatt aagaacactt aatttttctc taaatcccta
      481 ttgatttttt accaatgatc cccatttaga catttctgca atgtaattga ggattttatt
      541 ttggaacaat ttcatgggac aacaatactt tcagtacgac gcggtaatca ggttgtcatt
      601 ggcggtgatg ggcaagttac tctaggtaat accgtgatga aaggcaatgc acgtaaagtt
      661 cggagattgt ataaagataa agtgattgct ggttttgcag gtggaacagc agatgctttt
      721 actcttttcg aacggtttga agcaaaactg gaaatgcatc aggggcattt gattagagca
      781 gctgttgaat tggctaaaga ttggcgaacc gatagaattc ttcgtcgatt agaggcagtt
      841 cttgcagtag cggatagcaa ggcttcttta attattaccg gtaatggcga tgtcattgag
      901 cccgaagaaa gtttaattgc aatcggctct ggcggtccct ttgctcaagc tgcagccaga
      961 gctcttatgg aaaacacaca gttatctgcc aaggagattg tccagaaagc tttaacaatc
     1021 gctggcgaca tttgtattta tactaacaat aatttaacca tagaagaatt aaatgatgaa
     1081 ggcaaataat atggtcatga cccctcgaga gatagttcaa gaattagata agcacattat
     1141 tggtcaagat gatgcaaaac gtgccgtagc cattgcatta cgaaatcgct ggcgtcgaat
     1201 gaaaatcaaa gatccagtat tgcgcaacga gataatgcct aaaaatattt taatgatagg
     1261 ccctaccggt gttggtaaga cggagattgc aagacgtttg gctaatttgg ctaaagctcc
     1321 atttattaaa gtagaagcaa ccaaattcac agaagtggga tatgtagggc gtgatgttga
     1381 ttcgatcatt cgtgacttaa cagatatggc aatcaagcaa gagcgtgaat ttgccatgaa
     1441 aaaagtggag catctggcag aagatgcggc tgaagaacga atcctggatg ttttactccc
     1501 tcctgctcga ggaacattaa ctcctggtga gaaaaacacg acagctcgtc aagtgtttcg
     1561 taagcaattg cgcgaaggtg agcttaatga taatgaaata gaaattgaag tagcagcaac
     1621 gcctgttggc attgaaatta tggctcctcc aggtatggaa gagatgacta gccaattgca
     1681 gtctatgttt caacaagttg gaagctacag aacgaaaact cgaaaaatga ccgttgccaa
     1741 ggcaatgaaa attttgagag aggaagaagc ggcaaaatta attaacgaag aagatatcaa
     1801 gttaaaagcg attgaaagtg ttgagcaaaa tggaatagtg ttcattgatg agttggataa
     1861 aattgccaaa cgctctgata ctgtaagtgg gggagatgta tcacgtgagg gagttcagcg
     1921 agatttattg ccattagtcg aaggaactac cgtttcaaca aaatacggca tggtaaagtc
     1981 tgatcatatt ttattcattg cttcaggtgc ttttcatgta gctaaacctt ctgacttgat
     2041 tgctgaattg caagggcgtc tgccgatacg tgtcgagttg tcagccctct ctgtggaaga
     2101 ttttgtacga atccttactg agccaagcgc ctctttaact ttacagtatt ccgctttgat
     2161 ggaaaccgaa ggattgactc ttacctttga tgaaacagga attcgacgta ttgctgaagt
     2221 agcctggcaa gtgaatgagc gtacggaaaa tatcggagca agaagattat ataccgttat
     2281 ggaacgctta ctggaagtgg tttcatttga agcgactgat aaagctggcg aaacagtgca
     2341 tgttgataaa gcctatgtcg ataagaattt aggccaacta atcgctgatg aggatttggc
     2401 tcgctatatt ttgtaatcca gaatagttaa actgaaatca agaatgaaat ctttttaaca
     2461 gatcaacttt ttgtggggga tatttctcga aaaagagata tccctcatgt gattctgagt
     2521 gtccaaccat gtaatttact tttacaaaga gcaaggctat gctaattata ttctgatatc
     2581 attatgtaca taatggttgt ttatattaga aataaatgaa aactcagcga attatccatc
     2641 ccaatattga tattagaaaa tttcctgaag taaatgcaga tttttccatg accgatatta
     2701 gcatgggaga tttgcatgct aatgccttgc ttttcctcaa tattcttgtt cgccagggaa
     2761 taattgctat ttctcccgaa aattatgcca aatttgctga aatttatact ttgccagagc
     2821 ttcaggcaga ttactggggg acagaagcac ctgtttttag cgctggaaat aaacaggagc
     2881 gtttggagga aatcaagaaa caatacaatg cattaatagc gcaaatcaaa attattaata
     2941 ctaaaaaatt gattcgcttg ataggtgatg aattagtaga tcgcggtgtt attgactatt
     3001 tcattttaaa attattacaa gctttgaacg atcaaggtgc tgattttgaa atattacttt
     3061 caaaccatgg gattgagttt gtcgaagcct gtgaactatt caaggaaaat ggaaacaaat
     3121 tagtagcaaa aagactagga aatattcagc atggtaattc atttcatgct ttgcaagaag
     3181 caatagcagc tggttcgatt tcaaatgaag aagtattaaa tatttaccat caagtttaca
     3241 aaaaacactt gaaaattatt tcttacgcat tggatccaga tgcaaatgaa attaaagtgt
     3301 ttagtcatgc tggtattggt ttaaatcata ttcgaggact tgccagaaaa tttaaagtac
     3361 cttattcaga ggagtcggct gttgatttgg ctagaacaat tgatgcaatt aacaaaaaat
     3421 tcgcagaaaa agcatctgcg ggagaaattc acacgcttta tacccatgac atgatgtata
     3481 gaggttatgc aggtgaatat ttgaattcaa cagacgaagt ggttgccgca accgtatggg
     3541 gacgtgaata tggggatttg attcgaacgt ctaaaaaatt taaagtcacc tttattcatg
     3601 gccacgatag ttacgatcct gaaaaggtag aacatgtcac tttaaataat caactagggc
     3661 aattccaaaa taatgtaggt gatttatatc tgtatgccac caatggcatg agggtggttc
     3721 caacacaatg tttgaatcca gataaaaaag ttcagagcct aagggaaaaa aatcgaccag
     3781 ataaaccgaa tgattatgtt gtaaaagtcc atcatacaaa accatcattt tttaaaacgg
     3841 ctcatcccaa gatgacattc ccggacagct acaaacgaat atgggatagc acaccaggac
     3901 attccaatat aaccaaaatt aaggcattac ttaaagatta taccaaggaa gacaatattt
     3961 tgggttcttt ctggggatta atatttacat tgcactgggg aagacatcac gtcaaatccg
     4021 tgcatcagat tgcgcaaacc caatatacat cagtagaagc tattctttcg gatttgaaag
     4081 cactgaagcc aagagatggc ggttcactgg ataaacgcat taagtttatt gagtcacaaa
     4141 ttataactca gaggagtgat aatccagatt tacaattcaa tttgaaatag tctttttgtg
     4201 ttgtcttttc tcttaagtca tacttatttg ctttttaaat aacggtatca ttcaggtttt
     4261 aggaatttta ttcttgttat ccattcgatt gcaggggtta tcttggccct ggaataaacc
     4321 tgaaatcatt acatgtggtt atttgagtac tgttcctgtt ttcttgaata attcttccaa
     4381 attaatatcc agggattttt tcaattcttc attatgcctt ttaaaggcag ctctccattg
     4441 ttccgccaga gaggctttgg aatattgcag ctccagatga gttggcaagc gataaggtaa
     4501 caattgagtt aaatcataga gagtaacgtg actgagatca cgacttaaca tgtagtgccc
     4561 atcagcggta gcatggatca gttcatgata aatcagagca ttgatcattt catccacatc
     4621 cacagcgaaa ggttgtttgc tggcatccac cagatcatta aagcttaatc cttttccatg
     4681 ctgttgcgct atccataact ggtgcaacca gagcaacgca tgtgaaaagc catcgaggga
     4741 cttccctcca cgacgctgat gatgcactga aaaggcataa ctgatttcag cgcccaacaa
     4801 agtaataatc catacccaat aaacccaaat gaaaaatata ggtactgtgg caaatgcccc
     4861 atacaacaac tcataggtat tgtaacgtat caggtaataa gcaaatgcat gcttagctga
     4921 ttcaaacaag attgcggcaa ctaagccacc ccaaaaagca tgacggattt tgacaggaca
     4981 attgggaacc accacgtaca aaaaggtaaa accgatgagt gaaagaaaaa aaggggaata
     5041 gtgaagaata gtgtaaggag ctcgatgatc tgccaatatt ggcatggaga ataaatagga
     5101 gcttgctgcc aggctcaaac ctaataaaac cggggcaaga gaaatgatag cccaatagag
     5161 taaaaaggca gagacaccat gccgagatga actcactctc cagattttat tcattgctcg
     5221 ttcaatagtg aacatgacca ataacgcagt aaaaattaga aagactatac cccaaattga
     5281 gagttttgat acttgggagg taaattgttg caaatagctt tgcacaattt tacctgtggc
     5341 cggaacgaaa ttgtcaaaaa taaaattctg caccggctct gccaaacctt ggaatactgg
     5401 aaatgaagaa aatattgcca aacccacaga catcaatggc acgactgcca gtaagcttgt
     5461 aaaggctaat gccgaggcaa tataggtaca atcgtcttgt atgaaatgtt ggataacaaa
     5521 ccgaacaaat cgatcgcagg agtagaattt agtttttact ttttctttcc aattcatgga
     5581 ttaaatacaa gatttttatt gtataactat atcgttttat gcccgttttt gtatagttag
     5641 gatttacgaa aaactcaaat ctttttgtta aaaaagtttt taaatcggtc gtgtagtttg
     5701 tctaaactct cctgagcaaa tcaaatgaat aaaaggttat ttatatcatc atacaattta
     5761 gcaataacat aatacttctt tacagttttg tgatcatcca attactctat ccaatatagg
     5821 cgagtcattg acatatatca aaactattaa a
//
LOCUS       DATNSN010000178         5846 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.178, whole genome shotgun sequence.
ACCESSION   DATNSN010000178 DATNSN010000000
VERSION     DATNSN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.178"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            530..979
                     /locus_tag="VAH76_002733"
     CDS             530..979
                     /locus_tag="VAH76_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO1387320.1"
                     /translation="MNIPQETVVSETGDGQFTQKIIIGNHILTADEPIINGGKDTGPS
                     PYDFLLAALGSCTSMTLRMYAKLKKFPLEQVIVRLKHEKIHVDDCIGCENNNSKLDHI
                     ERLIELEGTLTQEQRTKLLEIADKCPVHRTLTSKIIITTKLVENQSK"
     gene            997..2037
                     /locus_tag="VAH76_002734"
     CDS             997..2037
                     /locus_tag="VAH76_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO1387321.1"
                     /translation="MKALIYQGPGKKALEDRPAPEIIHSTDAVIKITKTTICGSDLHI
                     LKGDVSTCQPGRILGHEGIGVVQKIGSGVTGFKEGEHVLISCITSCSKCEYCRKGMFS
                     HCVNGGWTLGNTIDGTQAEYVRIPFADTSLYKIPEGVDKEALVMLSDILPTGFECGVL
                     NGKIQPGNTVAIVGAGPIGLAALLTAQFYSPAEIIMIDLDDNRLEIAKRFGATATINS
                     HDGKAAEKVMKMTDGRGVDTAIEAVGIGKTFLICQDIVAPGGTIANIGVYGAKVDLHL
                     ERLWSQNIAITTRLVDTVTTPMLLKTVCAKKIDPTLLISHHFKFEDITDAYETFETAA
                     NTHALKVIIEVA"
     gene            2211..2423
                     /locus_tag="VAH76_002735"
     CDS             2211..2423
                     /locus_tag="VAH76_002735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387322.1"
                     /translation="MKLYEHPEVVDINNTCDISPPPQYTSDVCELVKANKVILLKTVT
                     TRIKNDRELDQALKDTFPASDAITKY"
     gene            2511..2675
                     /locus_tag="VAH76_002736"
     CDS             2511..2675
                     /locus_tag="VAH76_002736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387323.1"
                     /translation="MSHYDKKFEDESQDARRNDRHDEVHPDDKLVRHIVKPTDKVKST
                     NKDKPEDHKK"
     gene            complement(2878..3612)
                     /locus_tag="VAH76_002737"
     CDS             complement(2878..3612)
                     /locus_tag="VAH76_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387324.1"
                     /translation="MRVLAPSLLYVVLVSTIPVVAAFLGAVVAALRAPGLVVRSSIQH
                     FAAGVIFSVIAVELLPEVRRGHDPFEVGWTFAAGVVFMLIIDAAVKRFNHGGKQGAPS
                     GPSPGQLVPIGVDVLLDGILIGIAMTTGLKQGLLLTIALTLEFFSLGLTMALEFGTSK
                     LLVRVLAPTGIACLMIVGAILGRTLLADASVHTLAGLLAFGCAALLFLVTEELLIEAH
                     KSANTSVTTTMFFVGFLIFLLLGMVE"
     gene            complement(3655..5247)
                     /locus_tag="VAH76_002738"
     CDS             complement(3655..5247)
                     /locus_tag="VAH76_002738"
                     /inference="COORDINATES: protein motif:HMM:NF013739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1387325.1"
                     /translation="MPKIIIMGCGIGGLTVAHELVSSIDSTYEIHLYDRHNIIGGMAR
                     SGLKNRCDTMLPTEYCWRIYGPNYNNLRHILKQIPLKHDASKTVHDNLIDISNYLIAD
                     QQTVVHMNNRPGTLFDLRRVFKKVPLKQKIQVLNKIIYCFLISTKRLNALDKLPWANY
                     INPDNSLCHDMKKYIIDIMGPYLGADAEKVNVPSVAKTLESFKILNRPISVMNGPTNE
                     VWFEHWKIYLESKGVIFHLNSEITAIHVEEDEIKHILLADNTKVSGDEYFCSLPVESV
                     ARLESLKIPGISELAKRSYQLMMSIQLYFDKKINLPYQNTAMYIPDSPWQLVIEPQGS
                     IWQKSYNGIADLWSIGLCDSVHPGLLIKKPFVECSHEEIKQEVWYQILQSEFNQYLHL
                     DAVQIIDYNVWDTYVYNGKKLDTYEPKFSTNQGTFYLRPENKTHLKNMYFATAYTKTD
                     TDMFEMESATESGRRAAKILEPTIKVIGIQRPLFFSLYRLVDKIVPALNIYQYSPMLW
                     FLAGVIPALIVFPFLLLYRKIK"
     gene            5698..>5846
                     /locus_tag="VAH76_002739"
     CDS             5698..>5846
                     /locus_tag="VAH76_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HEO1387326.1"
                     /translation="MLGTIILVLLILFLVGGLPHWGYSRGWGYGPSGIIGLILVIYLV
                     LVLIG"
BASE COUNT         1836 a         1112 c         1159 g         1739 t
ORIGIN      
        1 tttattgtgc ttccggactc ttccctgttt tttcataatc ctctccattt atgaaagccg
       61 acaaatatgt gaatttcgat gtgttatcca atgttgttcg agttttcttg ataacaagct
      121 ttatgggaag attatttaac aaattcagtt cttttttagc gatgccgcct gctaggatga
      181 aaagtgagca aatctcttta tcttcagtta agatcttaaa gtcattatca atagtgaact
      241 tgagtattaa tctctaatta tagtagatga aatataaaac aaaaatcaaa acgccatttt
      301 agcaaatgcg ttttaaattt tgatcaaaat cgctatacca ttatttgttg tgtttatacg
      361 atgttctaca agacatgtac cacaatagga tataaggatg aatacactgg attttgttga
      421 aattgcagtt ggtactgaat attttgatgg gcaggactat ttagcatttt tcaaggaata
      481 ccacgcttac tcaggcagat cctgaattta ccgtaaggag attaacgtaa tgaacatccc
      541 tcaggaaaca gttgtctctg aaacaggaga tggtcaattt acacagaaaa ttattatagg
      601 aaatcatatt ttaacagctg atgagcctat tataaatggt ggaaaagata ctggtccctc
      661 accctatgat tttttacttg ctgcattagg ttcatgtact tcgatgacgt tacgtatgta
      721 tgcaaaactt aagaaatttc ctctggaaca agttattgtg agattaaaac atgaaaaaat
      781 tcatgttgac gattgtattg gatgtgaaaa caataattca aaactcgatc atattgagcg
      841 tttaattgag ttggaaggaa cactgactca agaacaacgt actaaactat tagaaatagc
      901 cgataaatgt ccagtgcatc gaacattaac gtccaaaatc attattacaa caaagctggt
      961 tgaaaaccaa tctaaataat cttaaggagc atcgcgatga aagcacttat ataccaaggg
     1021 cctgggaaaa aagctttaga ggataggcct gctccagaaa taatacattc aacagacgcg
     1081 gttattaaga tcacaaagac tacaatttgc ggctctgatc ttcatatact taaaggagat
     1141 gtatcaacat gccagcctgg ccgaattctt ggtcatgaag gcataggggt ggtccagaaa
     1201 attggttcgg gagtaactgg atttaaggaa ggagagcatg ttcttatttc ttgtatcaca
     1261 tcctgtagca agtgtgagta ttgtcgtaaa ggaatgttct cacattgtgt aaacggtggt
     1321 tggacccttg gaaatacaat tgacggtact caagctgagt atgttcgaat accctttgca
     1381 gataccagtc tttataaaat tcctgaaggg gtagataaag aagccctagt aatgctgagt
     1441 gatattttac ctacaggttt tgaatgtggc gtactcaacg gcaaaataca accaggtaat
     1501 actgttgcga ttgttggtgc ggggcccatt ggcttggcag cacttcttac tgcgcagttt
     1561 tattcacccg ctgaaattat aatgattgat ttggatgata accgtctgga aatagcaaag
     1621 cgatttggag cgacagcgac aatcaatagc cacgatggca aagccgctga aaaagtaatg
     1681 aaaatgacgg atgggcgtgg agtggacact gctattgagg cggtcggaat tggcaagaca
     1741 tttcttatct gccaggacat tgtggcacca ggtggtacta ttgcaaatat tggtgtctat
     1801 ggtgcgaaag tagatttaca tttagagcga ttatggtcac agaacatcgc cattactacc
     1861 cgacttgtag acacagttac tacacccatg ttgctcaaaa ctgtatgtgc aaaaaaaatt
     1921 gatcctactt tattgattag tcatcatttt aaatttgaag atattacgga tgcttatgaa
     1981 acgttcgaaa ctgcagctaa tacccatgca ctaaaggtaa taattgaagt agcatgatct
     2041 ccaaagcaac ccaattcact agaatagcta acgtcaggct atttatttaa gatccgagaa
     2101 gtgtgattag aggatatctg agggctttat tgtcttttat agagagacaa tggagtcccg
     2161 tttattcaaa taattgacta tacttaatga tacacactaa ggagatcata atgaaactct
     2221 atgagcatcc agaggtggta gatattaata atacttgtga catttctcca ccgccacaat
     2281 atacctccga tgtatgtgaa ctagttaagg ctaataaagt gattttattg aagactgtga
     2341 ctacacgcat taaaaatgac agagagttag atcaggcatt gaaggacact tttccagcaa
     2401 gcgatgctat tactaagtat taatagagtg ataattatca atagttcttc ataagggcac
     2461 gtctggccct aatttgcaca atcagttcaa ttttatttgg ggaaattagc atgagtcatt
     2521 atgataaaaa gtttgaagat gaatcacaag atgcacgtcg taacgatcga catgatgaag
     2581 ttcaccctga tgataaactg gtgaggcaca ttgttaaacc tactgataaa gttaaatcta
     2641 ctaataaaga taaaccagaa gaccataaga aataaacttg atacggaacg ctatattcga
     2701 cgttggggaa ttctggttag gacttacttg attctgctta cggtaaaact tagtaccatg
     2761 tttatgctct aatatataac aggtggagaa ggccgtccca ttatcacttc gcaaagttga
     2821 cgccctacat tgtcgcttct tcctctgttc agtaagtttc gaaatggcga tcggcggcta
     2881 ctcgaccatc ccaagtagga gaaagatcag gaatcccacg aagaacattg tagtagttac
     2941 ggacgtgttt gctgacttgt gcgcctctat cagtagttct tctgttacca aaaagaggag
     3001 cgccgcacag ccgaaagcaa gcagtcccgc cagggtatgc acagaagcat cggccaggag
     3061 ggtccgaccg agaatggcgc cgacgatcat caggcaagca attccagtcg gcgcaagcac
     3121 gcgcaccagc agctttgaag tcccgaactc caacgccatt gtgaggccga gagagaagaa
     3181 ctcgagcgtg agcgcaatgg tgagaagcag accctgtttc agcccggttg tcatcgcaat
     3241 gccaatgagg atcccgtcga gaagaacatc cacgccgata gggacgagct gcccgggtga
     3301 tggtccgctt ggagcgcctt gtttgcctcc atgattgaat cgcttcaccg ccgcgtcaat
     3361 gatgagcatg aacactaccc ctgccgcaaa cgtccagccg acctcgaatg gatcatgtcc
     3421 cctccgcacc tcgggcaaca gttccaccgc aatgactgag aagatgacac ccgcggcgaa
     3481 gtgttggatg ctgcttcgaa cgacaagacc aggtgcgcgc agagcggcaa ctaccgcgcc
     3541 aaggaacgcg gcgacaacag gaattgtcga gactaggacg acgtaaagta agcttggggc
     3601 tagtaccctc ataagtaaat gttaccgaac agagacgtaa ttaacataat tgtcctattt
     3661 tattttcctg tacagcaaca agaagggaaa tactattaaa gctggaataa cccccgctag
     3721 aaaccaaagc atcggactgt attgatatat atttaaggca ggaactattt tatcaactaa
     3781 acgatataga ctaaaaaata aaggtctttg aattccaatt acctttattg ttggctcaag
     3841 aatctttgct gcacgtcgcc cagattcagt agcactttcc atctcaaaca tatcagtgtc
     3901 tgttttagta taagcggttg caaaatacat attttttaaa tgagtcttat tttcaggacg
     3961 caaataaaat gtaccttgat tcgtactgaa tttaggctca taagtatcta gtttttttcc
     4021 attataaaca tatgtatccc agacgttgta atcaattatt tgcacagcat ctaaatgcaa
     4081 atactgatta aactctgatt gtagtatttg ataccaaact tcctgtttaa tttcttcatg
     4141 cgaacattct acaaaaggct ttttaattaa aagtcccgga tgaactgagt cacataagcc
     4201 aattgaccat aaatcagcaa ttccattgta acttttctgc caaatcgatc cttgcggctc
     4261 aatgactaat tgccatggac tatcaggtat atacatcgct gtattttgat aaggaagatt
     4321 aattttttta tcaaaatata attggatact catcataagt tggtaggagc gcttagctaa
     4381 ctcacttatt ccaggaattt ttaatgattc gagccgcgct acactttcca ctggtagcga
     4441 acagaaatat tcatcaccag atactttagt attatcagcc aaaagaatat gttttatctc
     4501 atcctcctca acatgaattg ctgttatttc agagtttaga tgaaaaatta cgcctttact
     4561 ctccaggtag attttccaat gttcaaacca cacctcattc gttggtccat tcatcacact
     4621 aatggggcga tttaagattt taaaagattc cagagtttta gcaacagagg gtacattaac
     4681 tttttcagca tcagctccta gatatggtcc cataatatcg atgatgtatt ttttcatgtc
     4741 atgacaaagc gaattatccg gattaatgta attagcccag ggcaatttat ccagagcatt
     4801 taatcgcttg gtcgaaatca aaaaacagta gattatttta tttaaaacct gaattttttg
     4861 ttttaatgga acttttttaa aaacccgtcg caaatcgaat aaggtgccgg gcctattgtt
     4921 catatgtact accgtttgtt gatcggctat caaataattg gaaatatcaa ttagattatc
     4981 atgtactgtt ttgcttgcat catgctttaa ggggatttgc ttcaggatat gccgtaaatt
     5041 attataattg ggcccataaa tacgccagca atattctgtt ggcaacatgg tgtcacatct
     5101 gtttttcaga ccacttctgg ccatgccacc aattatgtta tgtcgatcat aaagatgaat
     5161 ttcataagtt gaatctatgg agctaacaag ctcgtgagca accgtcaatc caccgatgcc
     5221 acagcccatt ataataattt ttggcataat ttaataacct gtcaaaaact gctaatcaat
     5281 cagtaaagca aagttttggt taattgtaca atcccaatca taatacttct tatcaatagc
     5341 aatacgcaca gatatggcca aaaattagac agatcgggtt atctgaacta tagttaacat
     5401 gtaggtaccg tggaagcatt tatgcagaaa tggtccaaca acataaggcc actcttgagt
     5461 ggttttatgc tatttactac cgctggttca caatccagaa atttagttct cttcaaagag
     5521 tgtaatcggt cattctttac atgctctgta tcgaaacttt gtcatacatt aattaatatt
     5581 tcatattagg ctgctcaacg aattttgcgg tatattatag attatcaatt cattcaaaaa
     5641 agtatcatca aaatgttcta tactttatat gtggtttaac tcgccaagga agatatcatg
     5701 ctaggaacaa tcatattagt tttattaatc ctttttctcg ttggtggttt acctcattgg
     5761 ggttatagta gaggatgggg ttatggacca agcggtatta tcggacttat attggtaatc
     5821 tatctagtac ttgttttaat tggcag
//
LOCUS       DATNSN010000179         5789 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.179, whole genome shotgun sequence.
ACCESSION   DATNSN010000179 DATNSN010000000
VERSION     DATNSN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.179"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            117..2048
                     /locus_tag="VAH76_002740"
     CDS             117..2048
                     /locus_tag="VAH76_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEO1387327.1"
                     /translation="MNTQDFLAGYTKRFVDNKEEDMSLDEYLELCKTDPTAYANPAER
                     LLMAIGEPELIDTRHDPVLSRIFSNKIIHHYEVFKDFYGMEEPIEQIVGFLKHAAQGL
                     EETKQVLYLLGPVGGGKSSIAEKLKDLMERIPFYAIKGSPVFESPLSLFNPLEDGELL
                     RERFGIPTRYLRYLMSPWAVKRLQEYNGDISKFRVIKVKPSRLKQIAIAKTEPGDENN
                     QDISSLVGKVDIRKLEEFSQDDPDAYSYSGGLCRANRGLLEFVEMFKAPIKVLHPLLT
                     ATQEGNYNATEGLSAIPFEGIILAHSNESEWQSFRNNKNNEAFIDRINIVKVPYCLRV
                     SEEIKIYQKLIHNSSLANAPCAPGTLDMLAQFSVLTRLKEPQNSSIYSKMRVYNGESL
                     KDTDPKAKSYQEYRDFAGVDEGMSGISTRFAFKILSKVFNFDHTEVAANPVHLMYVLE
                     RQIEQEQFPQELHETYLNFIKEYLATKYVDFIGKEIQTAYLESYSEYGQNIFDRYITY
                     ADFWIQDQDYRDPDTGEIFDRALLNLELEKIEKPAGISNPKDFRNEVVNFVLRARANN
                     RGKNPVWNSYEKLKSVIEKKMFTNTEDLLPVISFNAKASEEDKKKHEEFISRMVEKGY
                     TRKQVRLLCEWYLRVRKSQ"
     gene            2202..3467
                     /locus_tag="VAH76_002741"
     CDS             2202..3467
                     /locus_tag="VAH76_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEO1387328.1"
                     /translation="MSQLIDRRQNAGKKSTVNRQRFLRRYKSQIKKAVSEAVGKRSIT
                     EIDQGEQITIPAKDIYEPQFHRGHGGHIERVLPGNDNFIAGDRIKRPGGGGAGGAGGN
                     ASDSGEGEDNFVFELSREEFLELYFEDLELPDLVKKELARISTYKTVRAGVTTSGIPN
                     NINVLRSMKQATGRRVALASPYKKRLKEAEEELERLKQLTNPDKIDLLKLERDIEFFK
                     KKIQTVPFIDTIDLRYNHRVRVPSPSTQAVMFCVMDVSGSMDEAKKDIAKRFFILLYM
                     FLTKNYEKIELVFIRHHTSAKEVNEEEFFYSRETGGTVVSSALELLNTIIEARYPPQA
                     WNIYVAQASDGDNWNADSPYCQELLQEKIMPLLQYFAYIEIMPRHHQSLWEVYQQVKE
                     RYPNFAMENIDNVADIYPVFRELFKRKTV"
     gene            3464..4990
                     /locus_tag="VAH76_002742"
     CDS             3464..4990
                     /locus_tag="VAH76_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEO1387329.1"
                     /translation="MRKKPLSTGAEWTFELIQAYDREIARIAKSFNLDTYPNQIEIIT
                     AEQMMDAYSSVGMPIGYHHWSFGKHFVSVEKSYKRGQMGLAYELVINSNPCISYLMEE
                     NTMAMQALVIAHACYGHNSFFKNNYLFKMWTSADAIIDYLVFAKKYISDCEERYGIDA
                     VESVLDACHALMNYGVDRYKHPTPLSIQEEKIRQQNREIYMQSQVNELWRTIPFSKQI
                     DKNGQKKRFPEEPQENILYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQGQTKVMNEG
                     WACFWHYTILHALYDEGLVTDEFMLEILQSHTNVIMQPAYNSPYYNGINPYTLGYHMM
                     QDIKRICENPTDEDKKWFPYLANTDWLTSLDTAMRNYKDESFIAQYLSPKLIRDLKLF
                     HIVDDDRNSELYINAIHDEWGYQQIREALSRQYNLGYLEPDIQVYSVDLQGDRSLTLR
                     YSQQNRVPLGSSTNEVLKHLHYLWQFPVILQAVDGENKITEEFSCPPITDSKPSNKEI
                     "
     gene            complement(5118..>5789)
                     /locus_tag="VAH76_002743"
     CDS             complement(5118..>5789)
                     /locus_tag="VAH76_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387330.1"
                     /translation="IKNGDKTLGDYIIPEQVKQIWAIIKNAAAKYFTENLRDDDYQAF
                     LNKGFKIIPIFYYQQQFPEITPEQYIQGLRPIELTRERDEIKEAFHRNLATDVAIPEF
                     AANDDLKTRLNEIKTHILTTEWKVGNYLLFKGGVMHGNKRLPHRVNDVLDLIEKVEQG
                     KLAPKVAYAQIVEKAKEALDNPRKGRFSETTNFYQDIYNHHILSDDYQFNHTVELTTD
                     QVRLL"
BASE COUNT         1904 a         1099 c         1138 g         1648 t
ORIGIN      
        1 agtggttagt ctactatact atgaattaaa agaagtaatt tttgctttta tacaggaaag
       61 agtaaaaaaa gaatcacatt atatccttct cagggttaag taaaagggga tggcgcatga
      121 atacacaaga ttttttagcg ggttatacca aacgatttgt tgataacaaa gaagaagaca
      181 tgagtttgga tgaatatctg gagttgtgca aaacggaccc gacagcatat gccaacccag
      241 cagaacgttt gcttatggcg ataggcgagc ctgaattgat cgacacccga catgatccgg
      301 ttctatcacg aattttctca aataaaataa tccatcatta tgaagttttt aaagattttt
      361 atggaatgga agagcctatt gagcaaatcg ttggcttttt gaagcatgct gcgcaagggt
      421 tagaggaaac aaaacaggtt ttgtatcttt tggggcctgt tggcggtggt aaatcttcca
      481 ttgcagaaaa attgaaagat ttaatggaaa gaatcccttt ttatgcaatc aaaggttctc
      541 ctgtctttga atctccactg tcattattta accccttgga agatggtgaa ttacttagag
      601 aacgttttgg tatcccaaca cgctacctac gctacttaat gtctccctgg gcagtcaaac
      661 gattacagga atacaatggc gacatcagca aattccgggt tattaaagtc aagccttctc
      721 gactcaaaca aattgccatc gcaaaaacag aacctggcga tgaaaacaat caagatattt
      781 cttctctggt tggtaaagtc gatatccgta aattggaaga attttcccaa gatgatcctg
      841 atgcttacag ctactctgga ggcttgtgtc gagccaaccg cgggcttttg gagttcgttg
      901 aaatgtttaa ggcaccgata aaggtccttc atcccttgct gactgcaacg caagaaggga
      961 attacaatgc cacagaaggg ctttcagcta ttccttttga aggaattata ctagcccact
     1021 ccaatgaatc agaatggcaa tcatttagaa acaataaaaa taacgaggca tttatagacc
     1081 gaatcaacat agtcaaagtg ccttattgcc tgcgcgtttc tgaagaaatc aaaatctacc
     1141 aaaaactaat ccataacagt tctttagcca atgccccttg cgcacctggt acactggata
     1201 tgctagccca gttttctgta ttaacccgtt taaaagaacc acaaaactcc agcatttact
     1261 ccaaaatgcg cgtgtataat ggagagagtt tgaaagatac cgatcccaag gcaaaatcgt
     1321 atcaggagta tagagatttt gcaggagttg acgaagggat gagcggaata tccacacggt
     1381 ttgccttcaa aatcctttca aaagtattca attttgatca tactgaagtg gcagccaacc
     1441 cggttcattt aatgtatgtg ctggaacgcc aaatcgaaca ggaacaattt cctcaagagt
     1501 tacatgaaac ctacttgaac ttcattaaag aatatttggc gaccaaatac gtcgatttta
     1561 taggcaagga aattcaaacc gcttacctgg aatcctattc tgagtatggg cagaacatat
     1621 ttgaccgtta cattacctat gctgatttct ggattcaaga tcaggattat cgcgatccgg
     1681 atactgggga gatatttgac agagccttat taaacctgga attggagaaa atcgaaaaac
     1741 cggcagggat ctccaatcct aaagacttcc gtaatgaagt agtcaatttc gtattgcgtg
     1801 ctcgagcaaa taaccgagga aaaaaccctg tatggaatag ctatgaaaaa ctaaaatcag
     1861 tgattgagaa aaaaatgttt accaatacgg aagatttgtt acccgttatt tctttcaatg
     1921 ccaaagcatc tgaggaagat aagaaaaaac atgaagagtt tatctctcga atggtagaga
     1981 aaggctacac acgaaaacaa gttcgcttac tttgtgaatg gtatttgaga gtaaggaagt
     2041 cacaataata ggtaggataa ttcataactc gataggttat gaaatacagt cagagttgta
     2101 agtaactatc tgaaaataat taagtagaat cctaaattca gatactgctt acaacctggc
     2161 taacaaaaaa ctgacctgca gaaggtgtgg agctaacaat tatgtcgcaa ttaattgata
     2221 gacgacaaaa tgctggaaaa aaaagcacgg ttaatcgcca acgtttttta cgccgttata
     2281 aaagccaaat taaaaaagca gtttctgagg ctgtcggaaa gcggagtatc actgaaattg
     2341 atcaaggcga acaaattacc ataccagcta aagacattta tgaacctcaa ttccacagag
     2401 gccatggtgg ccatatcgaa cgtgtactac cgggaaatga taattttatc gccggggatc
     2461 ggataaaaag acccggcggc ggtggtgctg gaggagctgg aggtaacgcc agcgatagtg
     2521 gtgagggtga agataatttt gtttttgaat tatctcgaga agagtttttg gaactctatt
     2581 ttgaagatct ggaattaccc gatttggtaa aaaaagaact ggcaagaatt agcacttata
     2641 aaacagtaag ggctggcgta actaccagtg gaataccgaa caacattaat gtccttcgct
     2701 caatgaaaca agcgacagga cgccgtgtcg ctttagcctc cccctataaa aaaagattaa
     2761 aggaagcaga agaagagctt gaacggctga aacaattgac caatccggac aaaatagacc
     2821 tattgaagct tgaaagagac attgaatttt tcaaaaaaaa gatacaaact gtaccattta
     2881 tagatacaat cgatctgcgt tataatcacc gtgtgcgtgt gccttcacca tctacacaag
     2941 cagtgatgtt ttgcgtaatg gacgtatcag ggtccatgga tgaagcgaaa aaagacatag
     3001 caaaacgctt tttcatccta ctctatatgt ttcttaccaa gaattacgaa aaaattgaat
     3061 tggtttttat ccgccatcac acctcagcca aagaagttaa tgaagaggaa tttttttatt
     3121 cgcgcgagac tggcgggact gttgtttcca gtgccctgga gttattaaat accatcattg
     3181 aggcacgcta ccctcctcaa gcctggaata tttatgttgc ccaagcatca gatggcgata
     3241 attggaatgc cgactctcct tattgtcagg agttgctgca ggaaaaaatt atgcctttat
     3301 tgcaatattt tgcctatatt gaaattatgc cccgtcatca tcaaagttta tgggaagtat
     3361 atcaacaagt aaaagaacgt tatcctaatt ttgccatgga aaatattgat aatgttgccg
     3421 atatttatcc ggtatttcgc gaattattta aaaggaaaac ggtatgagaa aaaagccttt
     3481 atcaactggt gcagaatgga ctttcgagtt aatacaggcc tatgatcgcg aaattgcacg
     3541 catcgccaaa agctttaatt tggataccta tccaaaccaa attgaaatca ttaccgctga
     3601 acaaatgatg gatgcctatt cttcggtggg tatgcctatc ggttaccacc attggtcttt
     3661 tggaaaacat tttgttagtg ttgagaaaag ctacaaaaga gggcaaatgg gcttggccta
     3721 tgagcttgta attaattcca acccttgtat ttcttattta atggaagaaa ataccatggc
     3781 tatgcaagcc ttggttatcg ctcacgcatg ctatggccac aattcttttt ttaagaacaa
     3841 ttacttattt aaaatgtgga cttctgcgga tgctattatt gactatctgg tattcgccaa
     3901 gaaatacatc agcgattgtg aggaacgcta cggaatagat gcggtagaat cagtgcttga
     3961 tgcttgccat gcccttatga attacggggt tgatcggtac aaacatccca cacccttatc
     4021 tatccaggaa gaaaaaattc gacagcaaaa ccgtgaaatc tacatgcaat cacaagttaa
     4081 tgagctatgg agaacgatac ctttcagtaa gcaaatcgat aaaaacggac aaaaaaaacg
     4141 ctttccagaa gagcctcagg aaaatattct ctattttata gaaaaaaatg cccctttatt
     4201 agagccttgg cagagagaaa tagtgcgtat agtaagaaaa ctggcccaat atttttatcc
     4261 tcagggacaa actaaagtga tgaatgaagg atgggcctgc ttttggcatt atactatttt
     4321 gcatgctttg tatgacgagg gtttagtgac tgatgaattt atgctggaaa tactgcaaag
     4381 tcacaccaat gtcatcatgc aaccagccta taatagccct tattacaatg gtattaatcc
     4441 atacaccctt ggctaccata tgatgcaaga catcaaacgg atttgtgaaa acccaacgga
     4501 cgaagacaaa aagtggtttc cctatctcgc gaataccgat tggctcacaa gccttgatac
     4561 ggcgatgcgc aactacaaag atgaaagctt tatcgctcaa tacctgtcac caaaacttat
     4621 acgagattta aaactgttcc atattgttga tgacgaccga aactccgaat tatacatcaa
     4681 tgccattcac gacgaatggg gatatcagca aatcagagaa gcattatcca gacaatataa
     4741 tctgggttat ctggaacctg atattcaagt ctactctgtc gatttgcaag gagatcgttc
     4801 tttgacttta aggtactccc aacaaaacag agtgccactt ggaagctcaa cgaatgaagt
     4861 attaaagcac ttacattatt tatggcaatt tccagtcata ttacaggcag tcgatgggga
     4921 aaataaaatc accgaggaat ttagttgccc acctattaca gatagcaaac caagcaataa
     4981 agaaatataa ttttaaagag ggttataaat aatcaatttg actataaatg atacttgtga
     5041 tgttttgaat ctgcaattaa tttaaaccag attcaagaca tgccccaagc gccagataat
     5101 tattttaacg ttcgaagtta caataaacgc acctggtcag ttgttaattc cacggtatgg
     5161 ttaaattgat aatcatcgct aaggatatga tgattgtata tgtcttgata aaaattggtc
     5221 gtttcagaaa agcgaccctt tcttggatta tccaaagcct ctttagcttt ttcgacaatt
     5281 tgtgcgtaag caactttagg cgctagctta ccttgttcga ctttttcaat caaatcaaga
     5341 acatcattga ctcgatgagg aagtcgtttg tttccatgca tgacgccacc tttaaacaac
     5401 aaataattcc ctactttcca ttcagtagtc aaaatatgtg tttttatttc atttaatctt
     5461 gtcttcaaat catcattcgc agcaaactcc gggatggcta cgtctgtcgc cagatttcta
     5521 tgaaacgctt ctttaatttc atctctttct cttgttaatt caattggtct taatccttgg
     5581 atatattgct caggagtaat ttcagggaat tgctgctgat aataaaaaat cggtataatt
     5641 ttaaaccctt tatttagaaa cgcttgataa tcatcgtctc tgagattttc agtaaaatat
     5701 tttgcagctg cattttttat aatagcccaa atttgcttca cttgttccgg gattatataa
     5761 tcacctaatg ttttatcccc attttttat
//
LOCUS       DATNSN010000180         5770 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.180, whole genome shotgun sequence.
ACCESSION   DATNSN010000180 DATNSN010000000
VERSION     DATNSN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.180"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1467
                     /locus_tag="VAH76_002744"
     CDS             <1..1467
                     /locus_tag="VAH76_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387331.1"
                     /translation="ILQSQIIPALTECSEALDKQNVTEALQALNKLPSEKEIGKTKGI
                     GQELRGQIVGVTQELTGNLEPLQRAVTTPVVKDAEKMRVRYETLVTDVTKRVTDFEKI
                     KPVNLDSYNKAIADLNNMQQELTLLRNEKIRMHTDKDKAVDFSDIEALEKRLQEAQPK
                     LLTQLLEQTSKDVAKLVKIPEKLTFTIIKSMTSKIKEQMDILELVRNERIKQHGGSTE
                     PLDMSDLDKLKGELQTLNQYLVDDLLEDIKDSLSNIKDIATFEEESKYIEKCLDHLTG
                     LEQTLDGSEKAIKQKEDISTYRKTLKDKQEKAYPEMVQLQYKSEGLIMQLRDLCNIHQ
                     GNVAEITKTRLQQLENQNSGGGLLGGLWTVTNTLGLTTDTVNVEKMQIRMKEQVLRGF
                     KTGLNNDKQNVDQIIGFLAKKKPSELEEGLGISKENATELHGLLQQLDSKTASIDKLQ
                     ENARLIDEISTKIGREPIKHDHVITMVEEENDDIRYGF"
     gene            complement(1588..1632)
                     /locus_tag="VAH76_002745"
                     /pseudo
     CDS             complement(1588..1632)
                     /locus_tag="VAH76_002745"
                     /inference="COORDINATES: protein motif:HMM:NF016529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            1681..2802
                     /gene="dupA"
                     /locus_tag="VAH76_002746"
                     /gene_synonym="laiE"
     CDS             1681..2802
                     /gene="dupA"
                     /locus_tag="VAH76_002746"
                     /gene_synonym="laiE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupA/LaiE"
                     /protein_id="HEO1387332.1"
                     /translation="MPIILDPEVLKVAEYVYQERLSKPYTEVGPEWEYNHKTPYATRA
                     TGTGHNLQRFITIDDQKLHRPIHGLAHTMRTLFYSQLMYEAAKRQPHPHRCADGRTIA
                     DLSVQDLKKLNIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEEYARKHLTHLFSEKE
                     IVLYSRCIEDRIGDRFDETAEGYLIHLSHMIDLMRCKSPVEVFIGHSRGVSGIVPTLI
                     QLFGREDGLDIMHYARSLFAATGEAVPYISSSEWPHLGIESDRVERALKIVGPLEVEG
                     QEADAKKTAQAGFSVDGCYSALVKVDTPDWYHQVKAKEDYDVDEVIALPQQVTIREEP
                     PKANESFLLSLLRHVFWCCPSNQKRDDENTEVLEKKAQL"
     gene            2856..5483
                     /gene="sidJ"
                     /locus_tag="VAH76_002747"
     CDS             2856..5483
                     /gene="sidJ"
                     /locus_tag="VAH76_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HEO1387333.1"
                     /translation="MFGFIKKVLDFFGVDQSEDNPSETAVETTDVSTKVKTTDTTTQE
                     ESSVKTKTVVPTQPMGSVKPETIAPDQQKKHQIKTETTTGTTKQKSSKVTLMDGHVKQ
                     YYFARRGETSTHDTSLPPPVKVLSGHSIPLKEIPFEATRNELVQIYLTSVDQLIKSNK
                     LNSVPSQQIASHYLFLRSLANSETDGIKKNQILSLAKPLGAYLATKEPHVWKMINELI
                     EKSEYPIIHYLKNNRAHSNFMLALIHEYHKEPLTKNQSAFVQKFRDSSVFLFPNPIYT
                     AWLAHSYDEDSSFNPMFRERLSTNFYHSTLTDNLLLRTEPKEVTLSSEHHYKKEKGPI
                     DSSFRYQMSSDRLLRIQGRTLLFSTPQNDVVAVKVQKKGEPKSTLEEEFQMADYLLKH
                     QRRLDVHSKLPQPLGQYSVKKSEILEISSESLDFERFKTLIDDSKDLEVYVYKAPLSY
                     FTYLHDKNQDLEDLTASVKTNVHDLFVLLREGIVFPQLADIFHTHFGEDEREDKGRYQ
                     ALVQLLNVLQFQLGRIDKWQKAVEYVNLRSSGLADLGDSLPITSLFTSSDFTKHYFSE
                     LLTGGYHPTFFDKSSGTANSLFTGKRRLFGNYLYLNTIAEYLLVIQLTLGSYGDKVTR
                     DMVDKPKKEAVWRELANVMFSSCAEAIHIMTGIPQSRALTLLKQRANIEKHFRQTQFW
                     MTPDYSKLDEDTLQMEQYSVYSGEPEYEFTDKLVSGVGLSVDGVHQDLGGYNRESPLR
                     ELEKLLYATVTLIEGTMQLDKEFFKQLEQVEKILSGEIKTDANSCFEAVAQLLDLARP
                     GCHFQKKLVLSYYEEAKLKYPSTPTDAYDSRFQVVAKTNATITIQRFWREARKNLSEK
                     SDIESEKPESERNSDKRLQ"
BASE COUNT         1990 a         1144 c         1115 g         1521 t
ORIGIN      
        1 atactgcaaa gccaaattat cccagccctc accgaatgta gtgaagcact ggataaacaa
       61 aatgtcacag aagcattaca agcgctcaat aagctgcctt cagaaaagga aataggtaaa
      121 accaagggaa taggacaaga attaagaggg caaatcgttg gtgttacaca agagctaacc
      181 ggaaatcttg agcccctgca acgcgcagtc actacccctg ttgtaaaaga tgctgagaaa
      241 atgagagtac ggtatgaaac ccttgtcaca gacgtgacaa aacgagtcac cgactttgaa
      301 aaaatcaaac cggtcaatct tgacagttat aacaaagcca ttgccgattt aaataacatg
      361 caacaagaat taactcttct gcgcaatgaa aaaattcgca tgcatacaga taaagacaaa
      421 gcagtggact tttctgacat agaagccctg gaaaagagat tacaagaagc acaacccaaa
      481 ttgctaactc aacttttgga acaaacatcc aaagatgtcg caaaattagt aaaaatacca
      541 gaaaagctga cctttacaat tatcaaatca atgacatcca aaattaaaga gcaaatggat
      601 atattggaat tggttcgtaa tgaaagaatc aaacaacatg gaggatctac ggagcctctc
      661 gatatgtctg atttggataa attaaaaggt gaattacaaa cattgaatca atacctggtt
      721 gatgatttat tggaagacat taaagactct ttgagtaata tcaaagatat cgctactttt
      781 gaagaagaat caaaatacat tgaaaaatgt cttgatcatt tgacagggct tgaacaaacg
      841 ttggatggct cagaaaaagc aatcaagcaa aaagaagaca tcagcactta tagaaaaacc
      901 ttaaaagata agcaggaaaa agcctatcca gaaatggtac aacttcaata taagagcgaa
      961 gggctaatca tgcaattacg tgatttgtgt aatattcacc aggggaatgt agccgaaata
     1021 acaaaaacaa gacttcaaca actggaaaat caaaatagcg gaggaggact cctgggtgga
     1081 ttatggaccg ttaccaatac tctgggtctt actacagaca ctgtaaatgt tgaaaaaatg
     1141 cagataagaa tgaaagaaca ggttcttaga ggatttaaga caggtctcaa taatgacaaa
     1201 caaaatgtag atcaaatcat tggcttttta gcaaagaaaa aaccatcgga gttggaagaa
     1261 ggtttaggca tatcaaaaga gaatgcaaca gagttacacg gactactaca gcaactggac
     1321 tctaaaactg cctcaataga taaattgcag gaaaacgcaa gattaattga cgaaatctca
     1381 accaaaatag gtagagaacc aataaaacat gaccatgtca tcacaatggt tgaggaagaa
     1441 aatgatgata taaggtatgg tttctaagga gtatttttta agtgcttgtt caattgttaa
     1501 atgatgtaat gaatagtgca aagctgtggc agatgagaaa gcaaaacatc atattatttg
     1561 tttctcatca agctgctgct ttttatttta aggaatacta aatttcctta ttgaataata
     1621 attattcgtg ttgtagcatt aaagaatgct atgttttaat ttaaacaaaa ggtggttatt
     1681 gtgcccataa ttttagatcc agaagtatta aaagtagcag agtatgtcta tcaagagaga
     1741 ttatccaaac cttacactga agtaggcccg gaatgggagt acaatcataa aaccccttat
     1801 gccactcgcg ctacaggcac agggcataat ttgcaacggt tcatcacgat agatgatcag
     1861 aaacttcatc gccccattca tggtttggct cataccatgc gcactctttt ttacagtcaa
     1921 ttaatgtatg aagcagccaa aagacagcca caccctcatc gatgtgctga cggacgcact
     1981 atcgctgatc tgagtgtgca agaccttaaa aagcttaaca tcgcacaatt attttttgtg
     2041 gctggacgag aaagcgaagc ctcttatggt gatgcttatc accgatatca tctgtatggc
     2101 gctaaacaat tcgaagaata cgcccgtaaa catttgaccc atctcttttc agaaaaggaa
     2161 attgtccttt attctcgatg catcgaagat cgaataggcg atcggtttga tgaaactgct
     2221 gaaggctatc tcattcactt atcgcacatg atcgatttaa tgcgctgtaa aagcccagta
     2281 gaagtgttta taggccattc acgcggtgtt tcaggtatcg taccaacctt gattcaacta
     2341 tttggtcgtg aagacggttt agatattatg cattatgcac gcagcctttt tgctgccact
     2401 ggcgaggctg ttccttatat cagttcatcg gaatggcccc atttgggcat tgagtctgat
     2461 agagtggaaa gagcgcttaa gattgtagga cctcttgaag tggaaggtca ggaagctgat
     2521 gctaaaaaaa ccgcacaagc tggattttcg gtggatggct gctatagcgc tttggtcaaa
     2581 gtagacacac ccgattggta tcatcaggtt aaagcaaaag aagactatga tgtcgatgaa
     2641 gtgatcgctt tgccacagca agtcactata agagaagagc ctcccaaggc aaatgagtct
     2701 ttcctgttat cactccttag acacgtattt tggtgttgtc cttcaaatca aaaaagagac
     2761 gatgagaata cagaggtctt ggaaaaaaaa gcacaactat gaaagaggtt atgaggcagc
     2821 ttattttata attttcgagg tcatgaggac gcagtatgtt tggtttcata aagaaagtac
     2881 ttgatttttt tggtgttgat caatctgaag ataatccatc tgagactgcg gtggaaacaa
     2941 ccgatgtttc aacaaaagtc aaaaccactg acaccaccac ccaagaagag agttcagtca
     3001 aaaccaaaac ggttgttcct acccaaccga tggggtctgt caaaccagaa acaattgctc
     3061 ctgaccaaca aaaaaaacac caaataaaaa ccgaaacgac tacaggcacg actaaacaaa
     3121 aaagctctaa agtaacacta atggatggtc acgtgaaaca atattatttt gcccgccggg
     3181 gtgaaacaag cactcatgac acctctctgc caccacctgt gaaggtgtta agtgggcact
     3241 ctatcccatt aaaagaaatt ccttttgaag ccaccagaaa tgaattagtc cagatttatc
     3301 tcacttctgt tgatcaactc atcaaaagca acaaattaaa ctctgttcca tcacaacaaa
     3361 ttgcctctca ctatctcttt cttagatcac ttgccaactc agagaccgat ggaattaaaa
     3421 aaaatcaaat actgtcttta gccaagccat tgggcgctta ccttgcaact aaagagcccc
     3481 atgtatggaa aatgatcaat gaactcattg aaaaaagtga atatcccatc attcattact
     3541 taaaaaataa ccgtgctcat tccaatttca tgctggcatt aattcatgag tatcataagg
     3601 aaccattaac caaaaaccaa agcgcctttg tacaaaaatt cagggattcc tctgtctttc
     3661 tcttccctaa cccaatttat acagcatggc tcgctcattc ctatgatgag gattccagct
     3721 ttaatcctat gtttcgtgaa agattatcca ccaactttta ccattccact ctgaccgata
     3781 atctcttgtt aaggacagaa cctaaggaag taactctttc atcagagcat cattataaaa
     3841 aggaaaaagg accaattgat tcgtcttttc gttatcaaat gtcaagtgat cggttattac
     3901 gtatccaggg cagaacttta ctgtttagta ccccccaaaa tgatgtggtt gcagtcaaag
     3961 tacaaaaaaa aggagagcct aagtccacct tggaagaaga attccaaatg gcagattatt
     4021 tgctgaaaca tcaacgtcgt ttggatgtac acagcaaact accacaaccg cttggacaat
     4081 actctgtcaa aaaatcagag attttggaaa taagcagtga atcattagat tttgagcgtt
     4141 ttaaaaccct aatagacgac tctaaagacc ttgaagtgta tgtttataaa gcccccctgt
     4201 cttatttcac ttacctacac gataaaaatc aggatttgga agatctgaca gcttctgtta
     4261 aaacaaatgt gcacgatctc tttgttctat tacgcgaagg aatcgtgttt ccccaattgg
     4321 ccgatatctt ccatactcat tttggtgaag acgagcgcga agacaaagga agataccagg
     4381 cattggttca acttttgaat gttttacagt tccaattagg gcgcattgat aaatggcaaa
     4441 aagctgtgga atacgtcaat ttgcgcagca gtggtctggc tgatctggga gacagtctgc
     4501 ccataaccag cctgttcact tcatctgatt tcacaaaaca ttatttctcc gaactgctta
     4561 caggaggata tcacccaacc ttctttgaca agtcatctgg tacagccaac tctttattca
     4621 ccggcaagcg ccgattattt ggtaattact tgtatctcaa taccatagca gaatatttgc
     4681 tggttattca gttaacactt ggcagctacg gggataaagt gactcgagat atggtggaca
     4741 aacctaaaaa agaagcggta tggcgggaat tggcaaacgt catgttttca agttgtgcag
     4801 aagccattca tatcatgact ggaatacctc aatcgcgagc actgacgcta ctaaaacagc
     4861 gcgctaatat tgaaaaacat ttcagacaga cacaattctg gatgacgcct gattattcca
     4921 agttggatga agatacactg caaatggaac aatacagtgt ttattccggt gaaccagaat
     4981 atgaattcac tgataaatta gtctctggtg ttggattgtc cgtcgatggt gttcatcagg
     5041 acttgggtgg atataacagg gaaagcccat taagagaact tgaaaaactg ctctatgcca
     5101 cagtaacact gattgaagga accatgcaac ttgataaaga attttttaaa caattggaac
     5161 aagtcgaaaa aatactttct ggtgagataa aaactgatgc caattcgtgc tttgaagcgg
     5221 ttgcccaact tctggacctt gcaaggcctg gatgccattt tcagaaaaaa ttggttttgt
     5281 cctactacga agaagccaag cttaaatatc cctccacacc aactgacgcc tatgattcac
     5341 gttttcaagt agtcgctaaa actaatgcca caataaccat tcaacgattc tggcgagaag
     5401 cgcgcaaaaa cttgtcagaa aaatcggata tcgaatctga aaaaccggaa tccgaacgta
     5461 actctgataa acgcttgcaa taagatggat agtctgcttc tgactccttg aaaccacgcc
     5521 atccccatgg agaagtcgaa gagacaggat agacttctcc atttttgact tttactttaa
     5581 gagaaataag ggaaaaccac tcagttctgt tccagctaac aactcgatat gttgtccatt
     5641 ttttaagcgc ttaatacttt cttaatactg tagtcctata atgaaaataa acataagaat
     5701 attcacatct attagagcca gtatttgtac atacaaatat ccgtttggga gaaagtaaaa
     5761 tgcctaaata
//
LOCUS       DATNSN010000181         5711 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.181, whole genome shotgun sequence.
ACCESSION   DATNSN010000181 DATNSN010000000
VERSION     DATNSN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.181"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..517
                     /locus_tag="VAH76_002748"
     CDS             <1..517
                     /locus_tag="VAH76_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387334.1"
                     /translation="MKESGWKKAILKPAIAGGARHTYLFDQDSADHYQDVFESLINNE
                     SMLLQEFQENIVNKGEVSFMVFGGRYSHSILKKAKSGDFRVQDDFGGTVHPYEASQEE
                     REFVENVIAQCDELPVYARVDVMWDNNNKLCLSELEMIEPELWFRESSQAASAMADAV
                     CEHISGASEYK"
     gene            559..2232
                     /locus_tag="VAH76_002749"
     CDS             559..2232
                     /locus_tag="VAH76_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEO1387335.1"
                     /translation="MKTRIVSLLVVSMVSANLQAASENIIWQAGDNNLKKYNFSILQD
                     KKGFISITNKITPLNYQLKPVDHEGNRDSNDIRYQVYYKNIPVWGHELILHKASKSKV
                     FLTGVDVSEIEKDVHQTDGKLSAADIESQIIKHNNNSIVYKNIKKIIYLDSRKKAYLA
                     YHLSMYVNDPLHFVSAPNFIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLF
                     QHGDSMKDIPSLGKFEVTIKGKNCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNI
                     PAFSYPCSKESQYININDGDTSPANYSFSSVNDAMYFAGVTLEMYKEYYGVEKPLGDD
                     LPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNF
                     TRLHSNLVYNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPI
                     RYMDYPTKDGHSIEHFDDYHDDLDVHLTSGIFNRAFYLLVHQPNWTVRKAFQVMVDAN
                     IKYWTSGTSFEAASCGVIQAAIDRQYNKQDVIHAFKEVGVICPVIGLIN"
     gene            2427..3545
                     /locus_tag="VAH76_002750"
     CDS             2427..3545
                     /locus_tag="VAH76_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1654 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387336.1"
                     /translation="MMDEVKRIHARQKIASAKELEKRYCIKEAITEYLRAIDLFLQIS
                     NKTPEDINAIVNVHYDLATLYFNRRDYLSAGRHYEAAINQLIETPLKDDSYRLLTELY
                     IDLSDACYELMNQSAGDEAMANALKAFGLIQNKTFEEQQIGDPVANFKQFHIFYEKKL
                     STKSYLNSAKFMNHEYLLGEGQVARQQEQALFEQFENISISEIQQIDRSLENMLSQLS
                     LAAEPPTPNSVSGVDRVIFNPIFVNVTPSDSSYRGMAMQVLNLAKSYIQNQKISDAIA
                     TYQQAIKILNTIKSPIESDLQIVQELTQHIRYLQNKPSSTKDQFSSWYKEAQAPVIAT
                     ASEMGFFNYRPQEAFQVSDSFHSPNEMDVEYCDGNLRP"
     gene            3997..5412
                     /locus_tag="VAH76_002751"
     CDS             3997..5412
                     /locus_tag="VAH76_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEO1387337.1"
                     /translation="MNKGMNAFVFLIAVIAGFGGFLFGFDSSVIADVKDQVISQLALT
                     DWQWAQVVSSSLLGCILGIPISGLVADKLSRRSLLKVVALGFILGSSLCALAPGFVSI
                     LLGRFIIGICIGIASYIAPLFIAEIAPPHQRGTLVLINGLTITFGQAIAYLIGYFLHD
                     YSLMSWRYLFWIGSLPALVLFSGMYFVPHSPRWIMMKYGADATLKTLKQIRPVGYNIQ
                     QELTEISNNLVNPAPAYIQMFKKPIVFVLLLGVGLGLFQQFSGINALMYYGPVVFESF
                     GFSPVKNAILATFFLGLVNFIFTVVTLFYVDKLGRRFLLISGTLIASISLYLVSLLSS
                     NSLLVNKFWILGGLSFYVMGYCISLGSLFWVLISEIYPLSVRGLAMSIATVFQWGANF
                     VVSLLFLPIYQFSGQTVTFAMFATFCLLACWFIYYFVPETTSVSLEKLEDNLSSGKKI
                     RDIGQPVSKNIKIAKFNLIMD"
     gene            5415..>5711
                     /locus_tag="VAH76_002752"
     CDS             5415..>5711
                     /locus_tag="VAH76_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO1387338.1"
                     /translation="MKKKTCRIGIVGAGRIGRLHAENILFHLPQFELVAIADPQIDRN
                     WAEKLGIAIVSNQSEDVLCHPDLDAILIASPSHLHLEQIITASIHEKAVFCEKPI"
BASE COUNT         1778 a          952 c         1156 g         1825 t
ORIGIN      
        1 aatgaaggaa tcgggatgga agaaagcgat attaaagcct gcaattgcag gcggagcacg
       61 ccatacttat ttatttgatc aagattccgc ggatcattat caagatgtat ttgaatcatt
      121 aattaacaat gagtcgatgt tactccagga atttcaggag aacattgtaa ataaaggaga
      181 agtctctttt atggtgtttg ggggtagata tagccattct atactaaaaa aagcaaaatc
      241 tggcgatttt agagtacagg atgatttcgg tgggactgta catccttatg aggcatctca
      301 agaagaaagg gagtttgttg agaatgtcat agctcaatgc gatgagttac ccgtgtatgc
      361 acgagttgat gtcatgtggg ataacaataa taaattgtgt ttgagtgagc tggaaatgat
      421 agaacctgag ctttggttca gggaaagcag tcaagccgcg tctgctatgg ctgatgcagt
      481 atgcgaacac atatcagggg ccagtgaata taaataaacg ctgtaagaca caacaatagt
      541 caaagacagg gaaaaaatat gaaaacacgg atagtttcat tattagttgt ttctatggta
      601 agcgctaatt tgcaagctgc ttcagaaaat attatttggc aggcgggcga caacaattta
      661 aaaaaataca attttagtat cttgcaagat aaaaaaggat ttatatcaat tactaataaa
      721 atcacgccgt taaattatca gctgaaacct gttgatcatg agggcaatcg tgattctaac
      781 gatattcgtt accaggttta ttataaaaat attcctgttt ggggtcatga gctaatcctt
      841 cataaggcca gcaagtcaaa agtttttctg accggtgtcg atgtgtctga aattgaaaag
      901 gatgttcatc agactgatgg gaaattaagt gctgctgata ttgaaagcca gataataaaa
      961 cataataaca attcaatcgt ttacaaaaat ataaaaaaaa tcatttatct ggattcacgt
     1021 aaaaaagctt atctagctta tcacttatcg atgtatgtaa atgatccatt acattttgtc
     1081 tctgctccaa attttattat cgatgccaat tctggtgaga ttttaaaaag ctgggatgat
     1141 ttaactcata agaaggttgg tcagggcttg gggggtaatg tattcattct tccctatcgg
     1201 tcgggattgt ttcaacatgg tgattcaatg aaggatattc cttctctggg caaatttgag
     1261 gtaacgatca aaggaaaaaa ttgtgttgta gaaagccctg aggtaagggt cattaatgct
     1321 gcgtatactg atttggacaa gagatccttc cccgttctta gcattgtgga aatctataaa
     1381 aatataccag cattttctta cccatgtagc aaagaatcac aatatatcaa tattaatgat
     1441 ggagatacat cacctgcaaa ctacagtttt tcttcagtta atgatgctat gtattttgcc
     1501 ggagtcacac tggaaatgta caaagagtat tatggcgttg aaaaaccttt aggtgacgac
     1561 ttgcctttgc gagcctatac acatattaaa aattttgata atgcctttgc tgtgccttct
     1621 attaaaataa aaggattgta cttaatgcat caacaaattg tgattggtga tggggatact
     1681 caattaacag caccggcaca gagcacttta tcgcatgagt tgtctcataa ttttaccaga
     1741 ttacattcta acctggtcta taatgggcaa tccggaggaa tcaatgaagc attctcggat
     1801 atggcatcga ttgctatgca ggattacttg cgtaaagatt ttccatggta ttgggatggt
     1861 gaagattggt ctatagggcg tgaggctact ataagtggtg agccaattcg ttacatggac
     1921 tatccaacga aagatggtca ttctattgag catttcgatg attatcatga tgacttggat
     1981 gttcatctga ctagcgggat ctttaacagg gctttttatc ttttagtcca tcaaccgaac
     2041 tggacagtga gaaaggcatt tcaagtgatg gtagatgcca atataaaata ttggacatca
     2101 gggacttcat ttgaagcagc ctcttgtggt gttatccaag cagcaattga caggcaatat
     2161 aataagcagg atgtcatcca tgcttttaaa gaggtaggag tgatttgccc tgttataggt
     2221 ctcataaatt aaatacagca attcacgtga cttcgatact gattgctgat aaaaagagat
     2281 aatgatatta attcaacttt ccagctcaca accaattctt tttaatttca gacaattaag
     2341 gaaattccta tttgttttaa gcgggttgga ttattttaca ataagttgtc tttatatatg
     2401 gatctgaatt cgcattaaag gagtcaatga tggatgaagt aaagagaata catgccaggc
     2461 agaaaatagc ctctgccaaa gagcttgaaa agagatattg cataaaagag gcaattaccg
     2521 agtatttaag agcaattgat ttatttttac aaatttccaa taaaactcct gaggatatta
     2581 atgcaatagt taatgtacat tatgatctgg cgaccctgta ttttaataga agagattatt
     2641 taagcgccgg tagacattat gaagcagcga taaatcagct catagaaacc ccgctaaaag
     2701 atgactctta cagacttcta acagagttat atattgattt atctgatgcg tgttatgagc
     2761 tgatgaatca atctgctggg gatgaggcaa tggctaatgc actaaaagca tttggattga
     2821 ttcaaaataa aacatttgaa gaacaacaaa ttggggatcc agttgctaat ttcaaacaat
     2881 ttcatatttt ttatgaaaaa aaactatcaa caaagtctta tttaaactcc gcaaaattta
     2941 tgaatcatga atatctgctc ggcgaggggc aagttgcaag gcagcaggag caagcgcttt
     3001 ttgagcagtt tgaaaatata tcgattagtg aaatacaaca aattgatcgt agcttggaaa
     3061 atatgttatc gcaattgtct ttggcagcag aacccccaac ccctaattca gtctctggag
     3121 ttgatagagt tatttttaac ccgatttttg taaatgtaac gcctagtgat agctcttatc
     3181 gcggcatggc aatgcaagtt ctaaatctag ccaaatctta tatacaaaat caaaagattt
     3241 cagatgcaat agctacttat caacaagcca ttaaaatcct gaatacaatt aaatcaccta
     3301 tagaaagtga tctgcaaatt gttcaggagc taacgcaaca tataagatac ttacaaaata
     3361 aacctagctc tacaaaagat caattttcat cctggtataa agaagctcaa gctcctgtaa
     3421 ttgccactgc ttcagagatg ggatttttta attacaggcc acaagaggct tttcaggtat
     3481 cagattcatt tcatagccct aatgaaatgg atgtggaata ctgtgatggc aatttgcgcc
     3541 cataagtacg ttgctagttg agtgacgata attatttttg tgttcaaacg gcagataggt
     3601 aggactttag ggtccttgcc ttttgcagca atattgcaaa aattaccaaa ttaactttaa
     3661 ctgcccattt ctaatgggca acaatacctc tagaaatctc tgcatctcta gtataattaa
     3721 ttcatgcaac ttgtgattgc agtgcagcaa gagtttgcgt ggcatagggt gttgcaccct
     3781 ttgttgcatg aattaactac ttatggtgat ttaaattgga acgttggcta agcgtgtcct
     3841 tttcccgccc aaaagtgttt atttaatcag caaacctcca ttatcggggt aaagtattat
     3901 tttatcttca ataatttaca ctttgatatt tttctcgcaa cctgaccgtg ataacatcat
     3961 gcacgaacaa aattttcatt agggacagat agttacatga acaagggaat gaacgcattt
     4021 gtattcctga tcgcggtaat tgccggattt ggcggttttt tattcgggtt tgattccagc
     4081 gtcatagctg atgtgaaaga tcaggttatc tcccaattgg cactaacaga ctggcaatgg
     4141 gcacaagttg tcagcagtag tttattggga tgtatattgg gaatacccat aagtggtctt
     4201 gtcgcagata agttaagcag acgatcctta ttaaaagtag tagctttagg ttttatactg
     4261 gggagtagtt tatgtgcttt ggctcccggc ttcgtttcga tattactcgg acgatttatt
     4321 atcggcattt gcataggtat tgcctcctat attgctcctt tattcattgc agaaatcgcg
     4381 ccgcctcatc agaggggaac tttagtattg attaatggac taaccatcac gtttggtcag
     4441 gctatcgcct atttaattgg ctattttctc catgattatt ctttaatgag ttggagatat
     4501 ttattttgga tagggagtct acctgctctc gttttatttt caggaatgta ttttgtccct
     4561 cattcacccc gctggataat gatgaaatat ggagctgatg ctactttaaa gaccttaaag
     4621 caaattagac cagttggata taatattcag caggaattga ctgaaattag taataattta
     4681 gttaatcctg caccagccta tatccagatg tttaaaaaac caattgtttt tgttctctta
     4741 ttaggagttg gtttaggact attccagcaa ttttccggga tcaatgcctt gatgtattat
     4801 ggacctgttg tctttgaatc atttgggttt tctccagtta aaaatgcaat tctcgctacc
     4861 ttttttttgg gtctggtcaa ttttattttt acagtggtta ccctttttta tgttgataaa
     4921 ttaggcagaa ggtttttgtt aatcagtggt actttgatcg catcaattag cttgtatctt
     4981 gttagcttat tatccagcaa ctccttactc gtcaataaat tctggatttt aggcggtttg
     5041 tcattttatg taatggggta ttgcattagc ctggggtctc tgttctgggt tttaatatcc
     5101 gaaatatatc ccttatcagt gcgtggtttg gcaatgagca ttgccacagt gtttcaatgg
     5161 ggcgctaatt ttgttgtatc acttttgttt cttcccattt atcaattctc aggccaaaca
     5221 gttacctttg ctatgtttgc taccttttgt ttgctggcat gctggtttat ttattatttt
     5281 gtcccggaaa ccacatcggt atcgctggaa aaactggaag acaatttaag ctctggaaaa
     5341 aaaataagag atataggtca accagtatcg aaaaatataa aaatagcaaa atttaattta
     5401 attatggatt aatcatgaaa aagaaaacat gtcgaattgg gatagtaggt gcaggaagaa
     5461 ttggtagact gcacgcagaa aatattctat ttcatttacc gcaatttgaa ttagtggcca
     5521 tagcagatcc ccagatcgat aggaattggg cagaaaaact agggattgca atcgtgtcta
     5581 atcaatcaga agatgtacta tgccaccctg acttggatgc catattgatc gcttcaccat
     5641 ctcatttaca cctcgaacaa attattacag cgagtattca tgagaaagcg gttttttgtg
     5701 aaaaaccgat t
//
LOCUS       DATNSN010000182         5637 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.182, whole genome shotgun sequence.
ACCESSION   DATNSN010000182 DATNSN010000000
VERSION     DATNSN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.182"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..202
                     /locus_tag="VAH76_002753"
     CDS             <1..202
                     /locus_tag="VAH76_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEO1387339.1"
                     /translation="PFDIIKNKLIYFIEIVFVEMTGERQKHMYLWQKHFFSLMIRNAV
                     PDIQFYQLPYNKTTAIGTYYQF"
     gene            253..714
                     /locus_tag="VAH76_002754"
     CDS             253..714
                     /locus_tag="VAH76_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO1387340.1"
                     /translation="MSKADLHNCMLFKGVDGKYIDEFLENCEEVELPKGEFLFHQNEI
                     GDSMYIVEQGELQIILEQNSIGSDTKEEQLIGVFKSGALLGELCVFGQLKRAASIRAL
                     ADCRLLKIEGEDFRVRIYSKDLDALLICYNIAKLLSQRFITLLSLRHSPNA"
     gene            1037..5503
                     /locus_tag="VAH76_002755"
     CDS             1037..5503
                     /locus_tag="VAH76_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387341.1"
                     /translation="MPRYVEGVELTQEGMDAIFTRMGHSDIISGIIYNEQPTIDQEAL
                     NKQGFMPVLAGVGSRSDSGHWLMLIKGPGKQYYLFDPLGKTSGEGYQNTLLAQLPGGS
                     NLSVIPNGPDLNKGLCGYWVASVGLRAHAQLNTDSPPDLVNLGQTITNEMRNELEHDG
                     YRIITDWLRAVADEFPEGDPQPDARALREFTQKALGINIPPPVPPMKDLTPKELPVES
                     NCFQLPYVPVWNGFSLYTDDIVRAAAQYAYDNYLGKRYTGTVESVPANFGGQMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLRKFKDGRTIADVTPEELKKIMVAQAFFV
                     AGRDDEASDAENYRKYHEQSRDAFLKYVKDNESTLIPDVFKDEEDVNLYAQVIEDKNH
                     DWSSSPAIVLINQAHMVDLVRVKQPPESYLENYFKSMLPWIGPQATEAVFAIQRQFFH
                     ATHEVVAGFDSDNKEPHLVVAGLRRYVIGEDGQPIREAPKKGQKEGDLKAFPQAYQLK
                     GNERFMRVDEFLKLPEVQSTFPGSGKHLEGGMPGMNYMDYWNRLNSRERARCENDVDF
                     CLKQLEIAHHKAKIDPIKVAVQPSEKITRREPNIDEIAAAGIIREILANPDSIQNDHV
                     LINGQKLEEQFFRDLLAKCEMAVVGSLLNDTDIGNIDALMRHERDTEFHSTGEEAIPK
                     KIGAKWEKKQNKYNASRNITETKHNLIFLMQDDSWYFSRVNAIAQNRDKGSTFKEVLI
                     TTLMTPVTSKALVDTSRAPAPKRLFRGLNFSEEYAQGLINQANTMIANTTERLFTDHS
                     PEAFKQIKLNDLSKMSGRTNASTTTNIELCRTIWDSNVIFEMLDPDGLLQPKQVGQHG
                     AGTESEFSVYLPEDVALVPVKVTLDESKTENGGNRYIFTFVAVKSPDFIPRHESGYAV
                     EPFLRMQTAKLAEIKSSVKNNQVAPIIELQSDLSLVEVTDLPSAYKDFIKNTVVPVLE
                     NCLNVFLNKDAVALSKALAAFPPDGQWSVFNSPEAMHAKRQMDALKQMVPNKVVFDTL
                     AQCQDALEKQNIAGALEAFKNIPSEKEMGTISRELREQIQSTRQELESLQRAVVTPVI
                     TDEKKVRERYDALIEGTSKKVTELEKATLDKLDVIKKAISNLGNLRQEVTLLHNEKVR
                     MHSKTDTIDFSDIEKLEQQIQVMDTKLADAYLLAVTKQISALESIPKDQSELKAKIAT
                     FFDTITEIEMLRNERIKKYGASKDPHDLSDLDKLSGNLQGVNQSLVSNLIKTIRSSCI
                     QMKSNTFQMQKEAIEENIELLEKLEKSMDKSETAEKLRKDIPKLKDLLIAQQKAYPKM
                     TQLIEQLRELCQTHHDDLNKKRTARLQELDRQAAEGGITGIVGNIFFGVTNMIGLTKD
                     EQLEIKMKQQSLARFKADLFDEKNDIDALLVKLAYKSPSDLQEGLGISENNAKELCLL
                     IKNLAEKTYSANELEEKLKAIDEISPKLARECESLKFEAIDNDESTGNTMRF"
BASE COUNT         1884 a         1037 c         1193 g         1523 t
ORIGIN      
        1 accttttgat ataattaaga ataaattaat ctattttatc gaaatcgttt ttgttgaaat
       61 gactggagaa aggcaaaaac acatgtacct gtggcaaaag cacttttttt ctctcatgat
      121 acgtaatgct gtgccagata tacagtttta tcagttacca tataataaga ccactgcaat
      181 agggacttat tatcaatttt aaatccaatc gaagagagat tattggattt actagagttt
      241 aaggacagtg aaatgagcaa agctgattta cataactgca tgttattcaa gggcgtagac
      301 ggaaagtata tcgatgaatt tttagaaaat tgtgaagaag tggaactccc aaaaggtgag
      361 tttctttttc accaaaatga aattggtgat tcgatgtata tagtcgaaca aggcgagtta
      421 caaattatac tagagcaaaa ttcgataggc tctgatacta aagaggaaca attgatcgga
      481 gtatttaaaa gcggggcatt gcttggcgaa ttatgtgtat ttggccagct aaaacgagct
      541 gcatcaatcc gtgccctggc tgattgtaga ttattaaaaa ttgagggaga ggatttcagg
      601 gttcgtatct attcaaaaga tttggatgct ttattgatct gttataatat cgcaaaacta
      661 ttaagccagc gttttattac tcttttaagt ttacgtcatt ctcccaatgc gtgatttcag
      721 cagctagcgg attgctaatc ccagaattgt gttctcgtag ttttaacgct taagaaacca
      781 actgattttt ttattcttac ccttctgtag ttatttaatt tccaataact tccaagacaa
      841 cccggcagcc tcagcttgcc tcctatggag ccagtacaaa cgtatgatgt tgtcattctc
      901 aatatgacat taatgcataa ggcattagga atagtaatac tcaagtaata tttggttaat
      961 acctgaacag tagaataaaa gcataggtga atgtgctgat tagtacattt taaatattat
     1021 tttgggagaa accatcatgc ctagatatgt cgaaggggta gaattaactc aagaaggtat
     1081 ggacgctatt ttcacccgta tggggcatag cgatattatt agcggtatta tatacaatga
     1141 gcagccgact attgatcaag aagctctcaa caagcagggt tttatgccgg tgttagcagg
     1201 ggtaggctcc agaagtgatt ccggtcactg gctcatgtta atcaaaggac ctggtaaaca
     1261 atattatctc tttgacccat tgggtaaaac ttccggtgaa ggttatcaaa atacgttatt
     1321 agcccaatta cctggaggtt ctaatctctc tgtcatacca aatggccctg atttaaacaa
     1381 gggattatgc ggctattggg ttgcttcagt tggtttaaga gcgcatgctc aattaaacac
     1441 agatagtccc cctgatttag tgaatctggg gcaaaccatc accaatgaaa tgcgcaatga
     1501 actggaacat gacggttata gaataattac tgactggcta agagctgtag cagatgaatt
     1561 tccggaaggt gatccccaac ctgatgcgag agcattaaga gagtttacac aaaaagctct
     1621 tggtatcaat ataccccctc ctgttcctcc gatgaaagat ctaactccca aagagctccc
     1681 tgttgaatct aattgctttc aactgccata cgtgccagtt tggaatgggt tttcattgta
     1741 taccgatgac atcgttcgag ctgcggcaca atacgcctat gacaattatc tgggcaaacg
     1801 ttataccggg acagttgaat cagtaccggc aaattttggt gggcaaatgg tgtatagaca
     1861 acaccatggg ctttcccata ctttacggac catggcttat gcagaattga ttgttgaaga
     1921 agcacgtaaa gccaaattac gaggggaaac attaagaaaa ttcaaagatg gtcgtactat
     1981 tgctgacgtt acccctgaag aactgaaaaa aatcatggtt gcccaggcat tttttgtagc
     2041 tgggcgtgat gatgaagcat ccgatgccga gaactataga aagtatcatg aacaaagccg
     2101 tgatgctttc ttaaagtatg tgaaagacaa tgagtccaca ctaattcctg atgtcttcaa
     2161 agatgaggag gatgtcaatt tgtatgccca agtgattgaa gataaaaatc atgattggag
     2221 ttcaagtccg gccattgttt tgattaacca ggcacacatg gtcgatttgg tccgggtgaa
     2281 acaaccccct gagtcttatt tggaaaatta tttcaagtcc atgctgcctt ggataggccc
     2341 ccaggccact gaagctgttt ttgccataca aagacaattt tttcatgcta ctcatgaagt
     2401 ggtggcgggt tttgactccg acaacaaaga gcctcatttg gttgttgctg gtttacgtcg
     2461 ttatgtcatc ggtgaggatg ggcaacccat cagagaggca cctaaaaaag gacaaaaaga
     2521 aggtgattta aaagcttttc ctcaagctta tcaactgaaa ggaaatgaac gctttatgcg
     2581 agtcgatgag tttttaaaac ttcctgaagt ccaaagtact ttccctggtt ctggtaaaca
     2641 tttggaaggc ggaatgcccg ggatgaatta catggattac tggaatagac tgaattccag
     2701 agaaagagca cgatgtgaaa acgatgtcga tttttgttta aaacaattag aaatcgccca
     2761 tcacaaagcc aaaattgatc ctataaaggt tgctgttcaa ccgagcgaaa aaataacgag
     2821 gagagaacct aatatcgatg agatagctgc ggctggcatt attagagaaa tactcgccaa
     2881 tcctgattcc attcagaatg atcatgtgct cattaatggc caaaaactgg aagaacaatt
     2941 tttccgcgat ttgttagcca aatgcgagat ggctgttgta ggctcactgc ttaatgacac
     3001 cgacatagga aacatcgatg ctttaatgcg gcatgagaga gacaccgagt ttcattctac
     3061 tggtgaagaa gcaattccaa aaaaaattgg agctaaatgg gaaaagaaac aaaataaata
     3121 taatgctagt cgcaacatca ccgaaacgaa acacaattta attttcctca tgcaagatga
     3181 ttcctggtac tttagccgag tcaatgccat cgcacaaaat agggataaag gctccacttt
     3241 caaggaagtg ctgattacca ctttaatgac gcctgtaact agtaaagcac tagtggatac
     3301 atcacgcgcc cctgctccga aaagactgtt tcgtggatta aatttttcag aagaatatgc
     3361 tcagggatta atcaatcaag ccaatacaat gatcgccaat acgacagagc gtctatttac
     3421 agatcattca ccagaagcct ttaaacaaat caagttaaat gatttaagta aaatgtccgg
     3481 cagaactaat gccagtacaa ccacgaatat cgaactgtgt agaacaatct gggactccaa
     3541 tgtcatcttt gaaatgcttg acccggatgg tttgctgcag cctaagcaag ttgggcaaca
     3601 tggggcgggc actgaaagtg aattctccgt ttatttgcca gaggatgttg ccctggtccc
     3661 agtcaaagtg acccttgatg aaagtaaaac ggaaaatgga ggaaatcggt atatctttac
     3721 ctttgttgcc gtaaaaagtc ctgactttat acctcgccat gaaagcggct atgcggttga
     3781 accgttttta agaatgcaaa ctgccaagtt agccgaaatc aaaagctctg ttaaaaataa
     3841 ccaggtagcg ccaattatcg aattacaaag cgatttaagc ctagtggaag tgacagattt
     3901 accctctgca tataaagatt ttataaaaaa tacagtcgtc ccagttttgg aaaattgtct
     3961 taatgtattc ttgaacaagg atgcggtcgc tttaagtaaa gccttagcgg catttccacc
     4021 cgacggacaa tggtcagttt tcaactctcc agaagcaatg catgccaaaa gacagatgga
     4081 tgctcttaaa caaatggttc caaataaagt agtgttcgac accttggctc aatgccagga
     4141 cgcgctggaa aaacaaaata tcgctggcgc actggaggca ttcaaaaaca tcccatctga
     4201 aaaagagatg ggtaccatta gcagagagct aagagaacaa atccagagca ccaggcaaga
     4261 actggaatca ttgcaacgtg ctgtcgttac cccggtaatt acagatgaaa agaaagtaag
     4321 agaacgctat gatgccctga tcgaaggcac atcgaagaaa gttactgaac ttgaaaaagc
     4381 aacacttgac aaacttgatg tcattaagaa ggcaatttct aatttaggta accttagaca
     4441 agaagtgacg ctgttgcata atgaaaaagt acggatgcac agtaaaactg atacaattga
     4501 tttttcagat attgaaaaac tggaacaaca aatacaggtc atggatacca aattagccga
     4561 tgcttattta cttgcagtaa ccaagcaaat ttcagctctt gaaagcatac ctaaagatca
     4621 gtctgaattg aaagcaaaaa tagctacatt ttttgataca attaccgaga ttgagatgtt
     4681 gcgtaatgag cgaatcaaga aatacggtgc atcgaaagat ccgcacgatc tgtctgattt
     4741 ggataaatta agcggcaact tacaaggtgt taatcaatct ctggtttcga atctgataaa
     4801 aactatccgg agctcttgca ttcaaatgaa atctaatact tttcaaatgc aaaaagaggc
     4861 aatagaagag aatatcgagt tactcgaaaa gcttgaaaaa tcaatggata aatcagaaac
     4921 agcagaaaaa ctgaggaagg atattcctaa actaaaggat ttgctgattg cacaacaaaa
     4981 ggcctatcct aaaatgacac aattgattga gcaattacgc gagctatgtc aaacccacca
     5041 tgatgattta aataaaaaaa gaacggcgag attgcaagaa ttggatagac aggccgcaga
     5101 aggtgggata actggaatag taggaaatat attctttggg gttaccaaca tgatcggttt
     5161 aaccaaagat gagcaactcg aaattaaaat gaagcagcaa tcgcttgcgc gcttcaaagc
     5221 tgacttgttt gacgagaaaa atgatattga tgctcttcta gtgaagttgg catataaaag
     5281 tccatctgac ctgcaagaag ggttagggat atctgaaaac aatgccaagg agttgtgtct
     5341 acttataaag aatttggcgg agaaaactta ttctgcaaat gaattggaag agaaattgaa
     5401 agctattgat gagatctccc caaaactagc cagagagtgc gaaagtttga aatttgaagc
     5461 gattgacaat gatgagtcta ctggaaatac aatgagattc taattggatt ttttaagcca
     5521 gaacagtttt aacctctgaa attgggccga caaggcccag attacttcat ataacttcat
     5581 ataaatagta tccctgagag cacagctcta ctctgttcaa taaagattgc ataaggt
//
LOCUS       DATNSN010000183         5602 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.183, whole genome shotgun sequence.
ACCESSION   DATNSN010000183 DATNSN010000000
VERSION     DATNSN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.183"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            98..385
                     /locus_tag="VAH76_002756"
     CDS             98..385
                     /locus_tag="VAH76_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEO1387342.1"
                     /translation="MSAVMQQVTQGNDALSHQVISAVKGYLTSVGSKDANLNLYQLIV
                     EEVEAPLFRTVMELTRYNQSKAARVLGVSRGTLRTKLKRYFDDEFIGTRDF"
     gene            496..868
                     /gene="rnpB"
                     /locus_tag="VAH76_002757"
     ncRNA           496..868
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VAH76_002757"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(957..1745)
                     /locus_tag="VAH76_002758"
     CDS             complement(957..1745)
                     /locus_tag="VAH76_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEO1387343.1"
                     /translation="MKKLIYISLIFVISNAFAQPAATVLFAKQKVISLREGKEYSLSR
                     GSQLEPGDTVVTGAGALINIKYKNGTLVNIGENSRYKILAFSPNPSDVQIKAELTQGK
                     IKFKTTGKSNESLKTPVIAMAITGTSAEIFVKSDKQTYLNLLEGNINNYAPGSYIATP
                     QGVVAGAFPAEGQVNTPAGTDGSISDAETTDTISSESADATDTADTTDTADAADTADS
                     AQEASDAVGFIESAQVVDQATTDSIEEEIASEIAEISLECISVI"
     gene            complement(1765..2229)
                     /locus_tag="VAH76_002759"
     CDS             complement(1765..2229)
                     /locus_tag="VAH76_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387344.1"
                     /translation="MKTIRLFMLSMIAHFLLTCSVIHNVWAEKTLNPEKLANTTITLI
                     SNNQNASIANCSLQSVTSCTILLHLANPGTPGILHVTNHSSFVTATNIEAVLPSSLEF
                     LVDMIESGCDVVPPGSTCSIYFYPVSGPISPQPVQLIGDNIRTATFYLGIEM"
     gene            complement(2269..4515)
                     /locus_tag="VAH76_002760"
     CDS             complement(2269..4515)
                     /locus_tag="VAH76_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEO1387345.1"
                     /translation="MNKLTLYFSQFLNHTKWTEFFLGLTVTLLVLLTLFFNIPPISSL
                     ISQFEGRIYDQIINLNWHSKPENLKVIIIDIDEKSLEKEGRWPWPRNKIAELLNKLQE
                     NGVVTVGFDIVMSEAETNYALGLIEKLSQFSGKMTTDQKQLIATLNNVANQVDNDQML
                     VNALGKNNAILGFFFHNSEGIRKGALPTPLSDTDGNPLLANKLSVYQFTNFNGNLAAF
                     TAAATSGGFVTNLPDKDGVVRHALLLANYKNNLYPSLSLAIAMNYLLTDKVALKKYNN
                     QLTGIELDGLQIPTNRKGQILIPFWGGPGTLNYYSATDILNNQFNPEELAGSIALVGS
                     SITLLSDLHHTPVAQLFPGVEIVGNIVKGLVEQKIPREFDWESLQGKLCLVIFGLLFA
                     LLFSSIGIFGKLLIFLITTIAILTNTVILFVFYNNYIPPAFLLTITTLQTFVNFSYSY
                     VIEKRQKRRISELFGQYVPKEYVKELIEHPEHHTMEGLNREMTVLFTDIRNFTTISET
                     LGAGGVKLLLNSFFTPMTKIIFEHRGTIDKYVGDMIVAFWGAPIEDKEHASHAVLCSL
                     SCIEHLPQINTHLLEHGLPQVNIGVGIATGPMNVGDMGSEFRRAYTVLGDTVNLASRL
                     ESLTKFYQVNILVSDKTRSHLDSILWRTVDKVAVKGRKSPLTIYQPLGIVEQASARLL
                     QEQEKYETALAHYYNQDWLVAEILFNELKNVNPKIYLYQMYLERIAAFKKRGPSPDWD
                     GVYVHRSK"
     gene            complement(4714..>5602)
                     /locus_tag="VAH76_002761"
     CDS             complement(4714..>5602)
                     /locus_tag="VAH76_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEO1387346.1"
                     /translation="NDNKSAKNTNNFLKKITPEFAYSYIDFNFDSTAGLNFNRYNGHS
                     NLYSIGADHFSPVPSIIAGLYVLNIDTEVSSQFQLFPNTPIPSNQTIHNNTLFAHMRK
                     IFTPKFDIDIAGGYGQNRINSQTLLNRAIADIAFSKHTNDNWLASINAVYRKRGKKIS
                     LRTYVGMLYSQINSGSYILAFQSNQRGQIIAPLTTKTTYIMEGTEISYKLNSTLTPFV
                     NGGLIQIADFTNSRPILTQQINGSLPQLNMDKNGYKLGAGLIFNYKQMTLRLEEKYYS
                     AGNIFESYQTTAGLEYRFN"
BASE COUNT         1635 a         1023 c         1123 g         1821 t
ORIGIN      
        1 ctttgggata tttgatatta tatcggtcat ggtgttagag ccaaattaat gtgctggaca
       61 tgatttatgt tcattaaatt tgtattagga gattaaaatg agtgcagtta tgcaacaagt
      121 gacacaaggt aacgatgcct tgtcacatca agtgattagt gcagttaaag gttatttaac
      181 aagtgtcggt agtaaagatg ctaatttaaa cttatatcaa ttgattgttg aggaagtaga
      241 agccccttta ttccggactg ttatggaatt aactcgctat aatcaatcaa aagctgctcg
      301 agtgcttggt gtaagtcgtg gtactttgcg cactaaatta aagcgttatt ttgatgatga
      361 attcattggt actcgtgatt tttaattcag ttatatagct aatactttcc aagttcagtt
      421 atttttcgcg gtattctaat agaaattcat cgttaattat ggcctttgcc tgggcactct
      481 gctaatattg aagtcagagt cggtcaggca atcgctcttt gcctaacagc aaggggagga
      541 aagtccgggc tccatagggc agagtgccag gtaactcctg ggaggtgtga gcctacggaa
      601 agtgccacag aaaatatacc gcccactttt cctttgggaa tcgtaggtaa gggtgaaatg
      661 gtgcggtaag agcgcaccgc gcgattggta acagtcgtgg cagggaaaac cccactcgga
      721 gcaagaccaa ataggaatcc atttgactgc aaggtcctaa cgtatggccc atacgggatt
      781 cgggtaggtc gcttgaggca tgtggtgacg catgtcccag atgaatggtt gcccacgaca
      841 gaacccggct tatcgaccga ctctaatcca tattctatgt taatgcatac cattatatgc
      901 ctttggcccc tatataaata agggggtaaa gtcgcataca aaaatgacat gccaagctat
      961 ataacactta tacactccag ggagatctcg gctatttcac ttgcaatttc ttcttctatt
     1021 gaatcagtag tcgcttgatc cactacttga gctgactcaa taaatccaac agcatcagac
     1081 gcctcttgag cgctatcggc ggtatccgct gcgtcagccg tgtctgtagt atctgctgta
     1141 tctgtagcat ctgcagactc tgaggagata gtatcagtgg tttcagcatc agagatgctt
     1201 ccatccgtgc cagctggagt gtttacctgg ccctcagcag gaaaggcacc tgctacaacc
     1261 ccctgtggcg tagcaatgta actcccagga gcataattat ttatatttcc ttctagcaaa
     1321 tttaaataag tttgtttatc agattttaca aatatctcgg cagatgtacc tgtaattgcc
     1381 attgctatca ctggagtttt cagactttca tttgatttgc ctgtggtttt aaattttatt
     1441 tttccttgag ttaactcagc tttaatttga acatcagatg gatttgggga aaaggctaga
     1501 attttatatc tggagttctc acctatattt accaaggtcc catttttata tttgatgtta
     1561 atcagggcgc ctgcccccgt aacaacagtg tctcctggct ctaattgaga gcctcgagag
     1621 agtgagtatt ctttaccttc tctgagtgag atgacttttt gtttggcaaa taatactgtg
     1681 gctgctggtt gagcaaacgc attgcttata acaaaaatta gactaatata aattaatttt
     1741 ttcatgagtt cttcctactg gatatcacat ctctatacca agataaaatg tcgcagttct
     1801 tatattgtcg ccaattaact gaactggttg cggagaaata gggccactta caggatagaa
     1861 atagattgag caggtacttc caggtggaac tacatcacaa ccgctttcaa tcatatccac
     1921 aagaaactct agtgagcttg ggagtacagc ttcaatattg gttgctgtaa caaaagaaga
     1981 gtggttagtc acatggagaa ttcctggggt accagggtta gcaagatgaa gaagtatggt
     2041 acaacttgtg actgattgaa gtgagcaatt ggcaattgag gcattttgat tattagaaat
     2101 taatgtgatt gtggtatttg ccagtttttc gggatttagg gttttttcag cccaaacgtt
     2161 atgaattact gagcaggtta ataaaaaatg tgcaatcatt gataacataa acagcctaat
     2221 tgttttcata atccttccta ttgcagtatt taaaatttct tcttgatctc acttgcttct
     2281 atgaacatac accccatccc agtcaggtga aggccctctt tttttgaatg ccgctattct
     2341 ttccaaatac atttggtaca aataaatttt agggtttaca ttttttaact cgttgaagag
     2401 aatctcagcg acaagccaat cctgattgta atagtgagct aatgccgttt cgtatttttc
     2461 ctgctcctgc agaagtcttg cagaggcttg ttcaacaata cctagaggtt ggtaaatagt
     2521 caatggactt ttacgacctt tgaccgccac tttgtctacg gtacgccata atatgctatc
     2581 caggtgagag cgagttttgt cacttaccag gatattcact tgatagaatt tggttagact
     2641 ttctagcctg gatgcgaggt taacggtatc gccaagaaca gtataggctc gacgaaactc
     2701 agaacccatg tcaccgacgt tcataggacc agttgctata cctactccaa tattaacttg
     2761 tggcagtcca tgttccaata aatgggtatt aatttgaggc aagtgctcaa tgcaggaaag
     2821 agaacacaaa acggcgtgac ttgcatgctc cttgtcttct attggtgctc cccaaaatgc
     2881 tactatcatg tccccaacgt atttatcaat ggttcctcta tgctcaaaaa tgattttggt
     2941 cattggggtg aaaaatgaat ttaacaacag ttttacaccg cctgcaccaa gtgtttcgct
     3001 gattgttgtg aaattacgta tgtctgtaaa cagaactgtc atttcacgat tcaacccttc
     3061 catagtgtgg tgttcgggat gctctattaa ttcttttacg tattctttgg gtacgtattg
     3121 accgaacaat tcacttattc gtcttttttg cctcttttca atgacatatg aataggaaaa
     3181 attaacaaaa gtttgcaagg tagtgatggt taataaaaat gcagggggga tgtaattgtt
     3241 gtaaaatacg aacaggataa ctgtatttgt taatattgct atggttgtta ttaaaaagat
     3301 taataatttg ccaaaaatac caatagaaga aaataaaagc gcaaataata agccaaaaat
     3361 cactaaacaa agttttcctt gcaatgattc ccagtcaaat tctcggggaa ttttttgttc
     3421 caccagtcct tttacgatat tacccactat ttcaacccca ggaaataatt gggcaactgg
     3481 tgtatggtgt aaatcagata agagtgttat ggatgagcca actaaggcta tactcccagc
     3541 caattcctcg ggattgaatt ggttgttcaa tatgtcagtc gctgaatagt aatttagtgt
     3601 tcctgggcct ccccaaaatg gaataagaat ttgacctttt ctatttgtgg ggatttgtag
     3661 cccatctaat tctatacctg tcaattggtt gttatatttt ttcagagcaa ccttgtcagt
     3721 taagaggtaa ttcattgcta ttgccaaaga taaactggga tagaggttgt ttttataatt
     3781 ggccaataaa agtgcatgtc ttacaacccc atctttgtcg ggtaaatttg taacaaaccc
     3841 gccagaagtt gcagccgcag taaatgcggc caaattaccg ttgaaattag taaattgata
     3901 cactgacagc ttatttgcca ggaggggatt gccgtcagta tcacttaatg gtgtcggtaa
     3961 tgcccctttt ctaatgcctt cgctattatg aaaaaaaaaa ccaagtattg cattattttt
     4021 gcctaaagcg ttaacaagca tttgatcatt atcaacctga tttgcaacgt tgtttaatgt
     4081 ggcaatcaac tgtttttgat cagttgtcat ttttccagaa aattgactca gtttttctat
     4141 gaggcccaaa gcataattcg tttccgcttc tgacatgact atatcgaaac caacagtgac
     4201 cacaccattt tcttgtaatt tatttaataa ttcagctatt ttatttcttg gccatggcca
     4261 tcttccttct ttttctaatg atttctcatc aatgtctatg ataataactt tcaggttttc
     4321 aggttttgaa tgccaattaa ggttgattat ttgatcataa attcttcctt caaattggga
     4381 tattagtgaa ctaatcggtg gaatattaaa gaacaaggtt agcaatacca aaagggttac
     4441 tgtcagcccc aagaaaaact cagtccactt tgtgtgattg aggaattgag agaaataaag
     4501 agtcaattta ttcacaaaaa ttatcctttt gctactctta aatacaagat gaaagagcat
     4561 cctctttatt aatcagtata tagtctgaga ggaaagggca gaaataatgt ttttcagcct
     4621 tgtagatcac aggtgtgttt gctccatgtt attacaaaat tcattttcgc gcttctggta
     4681 tgaagaacaa taaataaact actataaaac taactaatta aatcggtact ccagtccggc
     4741 agttgtttga taactttcaa aaatgtttcc tgcgctgtaa tatttttctt caagccttaa
     4801 agtcatttgt ttataattaa aaataagtcc ggcaccaagt ttgtagccat tcttatccat
     4861 attgagctga ggcagagagc cattaatttg ttgagtaagt ataggtcgac tattggtgaa
     4921 gtccgctatt tgaatcaaac caccgttaac gaaaggggtt aatgttgaat tgagtttgta
     4981 actgatttca gtcccttcca taatataggt tgtttttgta gttaaaggag ctatgatttg
     5041 cccgcgttgg ttggactgga aggctaatat ataactaccc gaattaattt ggctgtataa
     5101 cattcccaca taggttctta gagatatttt tttccctcgt tttctataaa ctgcattgat
     5161 gctggcaagc cagttgtcat tagtatgttt tgaaaaagcg atatcagcga tggcacgatt
     5221 taacaaagtt tgcgagttta ttctgttttg accatatcct ccagcgatat ctatatcaaa
     5281 ttttggggta aatattttac gcatatgtgc gaataatgtg ttgttgtgaa tcgtttgatt
     5341 ggaaggaatt ggagtattgg gaaacaattg aaattgagaa ctcacttcag tatctatgtt
     5401 tagaacataa agccctgcaa tgatgctcgg tactggagaa aaatgatcag cgcctatgga
     5461 ataaagattg gaatgcccat tgtatcgatt aaaattaaga ccagctgtcg aatcaaagtt
     5521 aaaatctata tagctgtatg caaattcagg cgtgattttc tttaaaaagt tattagtatt
     5581 ttttgcgctc ttattgtcat tt
//
LOCUS       DATNSN010000184         5578 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.184, whole genome shotgun sequence.
ACCESSION   DATNSN010000184 DATNSN010000000
VERSION     DATNSN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.184"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2147
                     /locus_tag="VAH76_002762"
     CDS             <1..2147
                     /locus_tag="VAH76_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEO1387347.1"
                     /translation="RRQSWSHIDLGELAKNDSLLACLVLLTRYYQNPFSPPSLIARLP
                     LVNSKLSVDMFSRAAARASLDSEVKTLKLEQITDSDIPVVLIKKNGEAVLLILEDDGK
                     RAILDPKQPDSRIDLNQIADELSGEVIFVEQQYKPSTRAHETLSKEQKNWFWKVVIKS
                     WPLYSEVLVASLLVNIFALVVPLFSMNVYDRVVPNHAIETMWVLASGVALVFLFDMLL
                     KSLRSYFIDVANKHSDKELSALIFQQVLGLSMANRPKSVGSLANTVQSFEVFRDFITS
                     TTMTVLVDLPFVFIFLFVIYLIGGVLFWIPFLVLPTIFIIGLLLQWPLIRLTRKSYQY
                     GAEKQATLFESLATIETVKTTGAESVLQSRWERIINESSKNSMQLRFVANTSISITNL
                     AQQIATIAIIIAGVYMISEGELTTGGLIACTILTGRALAPMAQVSTLFTRYFQSVNAL
                     DSLNKIMQLETDTTEETRYLHRPSLKGAVEFKQVSFHYKEDETKVLQNVNFKINAGER
                     VAIIGRVGSGKSTLGKLILKLYVPTEGTILLDGTDYKQINPDDLRQQIGYVPQDIVLF
                     YGSIRDNICVGAPFVNDSALLEAAEIAGVTSFTNNHPKGFDRQVGEKGAELSGGQRQS
                     VALARALIMSPNILLLDEPTSAMDDNNEKQIKKNLNEYLSDNRTLILITHKISMLELV
                     NRIIVLENGKIIADGNKEAVLSALRTGVTVRK"
     gene            2151..3287
                     /locus_tag="VAH76_002763"
     CDS             2151..3287
                     /locus_tag="VAH76_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEO1387348.1"
                     /translation="MKKTHSSKTFTHIILWTSLLFFIVAIIWANYAILDEVTTGQGKV
                     IPSSEVQVIQNLEGGIIQNIFVKEGQIVKKDQILMQIDNTRFMASYAEAEKKIDALEI
                     EIIRLNAEISDTKPVFPDKFIKAYPHLVQDQLSLYESRMRELNQLKKSLELAQKELDL
                     TRPLLKGGSVSEVEVIRLERSVSEIKGNIEKFKSEELDKLNKARTELFALVEANKADK
                     DRLTRTTVRSPVYGIVKQIKTTTIGGVVQPGSDLLEIVPLDDTLLIEAKIRPSDIGFI
                     HPGQKAMVKITAYDFSIYGGLEGVVEHISADTIIDEKTDKKEESYYIVKVRTEKNYLG
                     TEKKPLPIIPGMQATVDILTGQKSVLQYLLKPIIKAKQSALRER"
     gene            3378..>5578
                     /locus_tag="VAH76_002764"
     CDS             3378..>5578
                     /locus_tag="VAH76_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1387349.1"
                     /translation="MPSMVLPKSSNAYPFAYYLTITFAMLTLILGLSVLIGWHFGIEF
                     LIQYQSGSVAIVYNTALCFIVLSLAILFSLNCYYKSSLILGITVFVFSGLGFSQHILD
                     VNLGIDEIFFHHYHAFKNAYPGRMAANTAFCFLMISAALIMMNLKCYRSFNNSLAGAL
                     GFLVFCLAILFISGYFSEIQHAYQWGVKTPMSINTAIDFFMLSSATIGLYWRNSIYFQ
                     LNSSIAMPYLCSFCIMLTFTLLSFEILEKENRLNLSFSIATFVIGIFLSFLFGLVIRL
                     WQLAKLSAIAAKYAFLEVEATLESTADAILVVDKEGNVINYNQRFLDMWRQRINPPRI
                     INYLDIKEEINKQLVNKREVIHRINQTIKHPDHQNSFELRLKGNLYYDCYTQPLKLDE
                     DIIGRVWSFRDITIPKRLEMELSHQSTHDTLTNLPNKVLAIDMLRYAMKGAISDKKIV
                     GVYLLDLDRFTQINDVFGHSKGDEIIKIIAKRLKDCVPVEHILGRISGDQFIVIASLD
                     EIREAVLGVTRLLAAFHEPVIMNSHCIKMSCSIGVSFYPRDGNTVDVLLSKADIALSR
                     TKIEGRNNFQFYTRDMNTYTLEHILLENELRNAIRKREFVLYYQPILELRSLNLNGFE
                     ALIRWNHPRNGLLLPEKFIQLAEEIGIISEIGDWVLTTVCKQIKTWQKQGVNNLKVAV
                     NISPKHLKFRHLPIRINEILSEFQVKSESIELELTENILIDKSSGIIDTLN"
BASE COUNT         1764 a          905 c         1085 g         1824 t
ORIGIN      
        1 cgagaagaca atcatggtct catattgatt tgggtgaact agccaagaat gactctctgc
       61 tagcctgtct ggtattatta actcgatatt atcaaaatcc gttttcgcct ccatcactta
      121 tagctcgttt gccattggtc aatagtaaat tgagtgttga tatgttttcg cgtgccgctg
      181 ccagagcctc tttggattct gaagtgaaaa ctttaaaatt agaacaaatc actgattcag
      241 atataccagt agtattaatt aaaaaaaatg gcgaggccgt gcttctaata ctggaggatg
      301 atggtaaaag agcaatcctt gatcccaagc aacctgattc ccggattgat ttgaaccaaa
      361 ttgctgatga gttaagtggt gaagtcattt ttgttgagca acaatacaaa cctagcacca
      421 gagcacacga aactctttcc aaagaacaaa aaaactggtt ttggaaagtt gttattaaat
      481 cttggccatt gtattccgaa gttcttgtgg cctccttgct cgtgaatata tttgcactag
      541 ttgttccatt atttagtatg aatgtgtatg accgtgtggt tccaaaccat gctattgaaa
      601 ccatgtgggt gcttgctagt ggtgttgctt tggtatttct ctttgatatg ctgctcaaga
      661 gtttacgctc ctatttcatt gatgtggcga ataaacacag cgataaagag ttgtctgctt
      721 taatatttca gcaagttcta ggattaagta tggctaacag gccaaaatcg gttggttctt
      781 tagccaatac agtacagtca tttgaagtat tcagagattt tattacttcg actactatga
      841 cagttcttgt tgatcttcct tttgtattta tttttctttt tgttatttat ttgattggcg
      901 gggtgttgtt ttggatacct tttttagtac tccctactat ttttataata ggtttgttgc
      961 tgcaatggcc tttaattcga cttacaagaa aatcctatca atatggtgct gaaaaacaag
     1021 cgacgttgtt tgagtcttta gctactatag aaactgtaaa gacaaccgga gcagaatcag
     1081 tcctgcaatc acggtgggaa agaataatta atgaatcttc taaaaactct atgcaattac
     1141 gttttgtagc caatacaagt atcagtatca ctaatctggc ccaacaaatt gcgactatcg
     1201 ctattatcat tgcaggtgtt tatatgatct ctgaaggtga gttaaccaca ggtggattaa
     1261 ttgcctgtac catattgacc ggaagagcat tggcgcccat ggcgcaggta tccaccctat
     1321 ttacccgtta cttccaatca gtcaatgctc tggactcatt aaacaagatt atgcaacttg
     1381 agacagatac cacagaagaa actcgttatc tccatagacc ttctctgaaa ggtgccgttg
     1441 aatttaaaca ggtgtctttt cattacaagg aagatgagac gaaggtatta caaaatgtca
     1501 attttaaaat taatgctggt gagcgcgttg caattatagg acgagttggt tctggtaaat
     1561 cgactttggg taaattaata ttaaagctgt acgttcctac cgaaggaact attttactgg
     1621 atggtaccga ttataaacaa attaatcctg acgatttacg tcagcaaata ggctatgttc
     1681 ctcaggatat tgttttattt tatggaagca taagagataa catttgtgta ggtgctccct
     1741 ttgtaaacga ctctgctctt ttagaagccg ctgaaatagc tggggtaacg agttttacca
     1801 ataatcatcc caaaggcttt gatagacaag taggagaaaa aggagctgaa ttatcaggag
     1861 gacaaagaca atctgttgct cttgcccgtg ctttgattat gagccctaat atcttgttat
     1921 tggatgagcc tacttcggca atggatgata ataacgaaaa gcaaatcaag aaaaatctta
     1981 atgaatactt atccgataat cgtactttga ttttaataac tcataagatt tccatgctgg
     2041 agctggtcaa tcgtattatc gttttggaaa atggcaaaat cattgctgat ggtaataaag
     2101 aagctgttct aagcgccctt aggactgggg taacggtgag gaaatagtct atgaaaaaaa
     2161 ctcacagctc aaaaaccttt acccatatta tcttatggac ttccttgctt ttttttattg
     2221 tagcaattat ttgggctaat tatgccattc ttgatgaagt aactacagga cagggaaaag
     2281 taattccctc tagtgaggtt caggttatac agaatcttga aggcgggatt attcagaata
     2341 tttttgtcaa agaagggcaa atagttaaaa aagatcaaat tttaatgcaa atcgataata
     2401 ctcggtttat ggccagctat gccgaagcag agaaaaaaat tgatgcattg gaaatagaaa
     2461 ttataaggct taatgctgaa attagtgata caaaacctgt gtttccagat aaatttatca
     2521 aagcctatcc tcatcttgtg caggatcagt tgtctctgta tgaatcacgg atgagagagt
     2581 tgaatcaact gaaaaaatcc ctagagttgg cgcaaaaaga attggattta actcgtccat
     2641 tattaaaggg tggctccgtt tcagaagtag aggttattcg tctggaaaga tcagtaagtg
     2701 aaattaaagg aaacattgag aagttcaaat cagaagaact ggataaattg aataaagcca
     2761 gaacagagtt gtttgcgctg gttgaggcga ataaagcgga taaagatcgt ttaaccagga
     2821 ccacagttcg ttctccagtt tatggtattg taaaacaaat taagacgaca acgattggtg
     2881 gtgtagttca accaggtagt gatttacttg agatagtccc gcttgatgat actttattaa
     2941 ttgaagcaaa aatacgccct tctgatattg gttttataca cccaggccaa aaagcgatgg
     3001 ttaaaattac tgcttatgac ttttctatct atggtggatt ggagggtgtt gtagagcata
     3061 ttagtgccga tactattatt gatgagaaaa cagataaaaa agaagaaagc tactatatag
     3121 taaaagtgcg aacagagaaa aactatttgg gaactgaaaa aaaaccattg cctatcattc
     3181 caggtatgca agctactgtt gatatactta caggtcaaaa atcagtcctt caatacctct
     3241 tgaaacctat aattaaagct aagcaatccg cacttagaga acgttgatga agttgttacg
     3301 gtattatgaa aaaatggtct atcattataa tattagtctt atttttgcaa ggatatcatt
     3361 gctagggata taacattatg ccatcaatgg tactccctaa atcatctaat gcttacccct
     3421 tcgcatatta tttaaccatc acctttgcca tgctaacact aattttgggg ttgtctgtct
     3481 taattggttg gcattttggt attgagtttt taattcaata ccaatcaggt tcagtagcaa
     3541 ttgtttataa tactgctcta tgctttattg ttttgtccct tgctattcta ttttctctaa
     3601 attgctatta taagtcgtct ttaattttag gtattactgt tttcgttttt tctggtttgg
     3661 gtttttccca gcatatttta gatgttaacc ttgggattga tgaaattttt ttccatcact
     3721 atcatgcctt taaaaatgct tatccaggcc ggatggcagc aaacaccgct ttttgctttt
     3781 taatgatatc ggctgctctt attatgatga atttaaaatg ctataggtca tttaataatt
     3841 cattagctgg tgcacttggg tttctagtat tttgtttggc aattttattc ataagtggat
     3901 atttctcaga aattcaacac gcttaccaat ggggagttaa aacccccatg tcaattaata
     3961 cggcaattga tttttttatg ttgtcctcgg caacaatagg actatattgg aggaacagta
     4021 tctattttca attgaattcc agtattgcga tgccttattt atgctcgttt tgtattatgc
     4081 taacttttac attactatca ttcgaaattt tagaaaagga aaataggtta aacttaagtt
     4141 tttcaatcgc aacatttgtt attggcattt ttctttcttt cctttttgga ttggttatca
     4201 gattgtggca actggcaaaa ctatcagcta tagcggctaa atacgcattt ttagaagtcg
     4261 aggctaccct ggaatcaaca gctgatgcta ttttggtagt ggataaagaa ggcaatgtga
     4321 ttaattacaa tcaacgtttt ttagacatgt ggcgccagag aataaatccg ccacgtataa
     4381 taaactatct ggatataaaa gaagagatta ataaacaatt agtgaataaa agggaagtaa
     4441 tccacagaat taatcaaact attaaacatc ctgatcatca aaattcattc gaattacgac
     4501 ttaaaggcaa tctttattat gattgctaca cccaaccact aaaattggat gaagatatta
     4561 ttggtcgcgt ttggagtttt agagatatta ctattcctaa aagacttgaa atggaattat
     4621 cccatcaatc tacacatgat acactcacca acttgcctaa taaagtgttg gctatcgaca
     4681 tgctgagata tgcaatgaaa ggtgcaatta gcgataaaaa aattgttggg gtgtatttac
     4741 tggatttaga tcgatttacg caaattaacg atgtatttgg tcatagcaag ggagatgaaa
     4801 taattaaaat tattgccaaa cgattaaaag attgtgtgcc agttgagcat atccttgggc
     4861 gaattagtgg cgatcaattt attgtaattg cgagtcttga tgaaattaga gaagctgttt
     4921 taggggttac acggttatta gctgcctttc atgagcctgt aataatgaat agccattgta
     4981 tcaaaatgag ttgcagcata ggtgtttcct tctatccaag agatggcaat acagttgacg
     5041 tgttgttatc caaagctgat attgctttgt ctcgaacaaa aatcgaaggc cgtaataact
     5101 ttcaatttta taccagagac atgaatacct atactttaga acatattcta ttagaaaatg
     5161 aattaaggaa tgctattaga aaaagagaat ttgttttgta ttatcaacct attctggagt
     5221 taagatcttt gaatttaaat ggctttgaag cattaatccg ctggaatcat cccagaaatg
     5281 gattgcttct ccctgagaaa tttatccagc tggcggaaga aataggaata attagtgaga
     5341 ttggtgattg ggtacttaca actgtttgta agcagataaa gacatggcaa aaacagggag
     5401 ttaataattt aaaggttgct gtcaatattt cgccaaaaca tttaaagttt cgacatcttc
     5461 caatacgaat taacgaaata ttatccgagt ttcaggtgaa atcagaatct attgaattag
     5521 agttaacaga aaatattttg atagacaagt cgagtggtat tattgatact ttaaatga
//
LOCUS       DATNSN010000185         5569 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.185, whole genome shotgun sequence.
ACCESSION   DATNSN010000185 DATNSN010000000
VERSION     DATNSN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.185"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            137..1072
                     /locus_tag="VAH76_002765"
     CDS             137..1072
                     /locus_tag="VAH76_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1803 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387350.1"
                     /translation="MSQYTRLFEFLCDLIDIQIKKYVELAKLKVGEDAIINAALIYEE
                     VNELLGFAEKLLKEIETNSKSDSMDMTIFDRVFEQVKFYVEQEYLRSYAEWLLIENNS
                     KDTVYNRTKEQLLRLSEIKSFDDKGFSQPSTEIQQQCQHDSVQIVYSILNLAREVRKN
                     PDMGFDDSIPHNKLNVLRRNATTRYVEFGNEIDKLHQHSEEFLQQSSLGVFDAELAYE
                     SALSKAKDHDLKLNTLFGRYKENKETASVPQSLSLVNKVDEENIMTEKKPSTEVSDNK
                     TPFFSTSNLLFFGGIVLAAVIFVPRLLTHLKVNKL"
     gene            1375..2421
                     /gene="recA"
                     /locus_tag="VAH76_002766"
     CDS             1375..2421
                     /gene="recA"
                     /locus_tag="VAH76_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO1387351.1"
                     /translation="MEENKQKALSAALSQIERQFGKGSVMRMGDSTVSRDIEAISTGS
                     LGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQKMGGTAAFIDAEHALDPS
                     YAQKLGVKVDELLVSQPDTGEQALEITDMLVRSAAVDVVIIDSVAALTPKAEIEGEMG
                     DSHVGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEILGSETRVKVVKNKVAPPFKMTEFDILYNEGISRESEIIN
                     LGVQLNLIEKSGAWYSYKQEKIGQGKENVRLYLKENPQVAAELEQQIRTELLEKKLSV
                     LASSSEDLFETIDD"
     gene            2414..2866
                     /gene="recX"
                     /locus_tag="VAH76_002767"
     CDS             2414..2866
                     /gene="recX"
                     /locus_tag="VAH76_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO1387352.1"
                     /translation="MTKAFDSALRLLTRREYSAMELCDKLKQKGFSTNDVQNALYECQ
                     RLGYQSDVRFVENYIRVRIHQGYGPLKIRQELKNKGIDPDLIQSVLHQEKDNWVNYAL
                     RAWEKKFKRQDDFSYSEIQKQQRFLLYRGFDRDVISKVFKEVKSSYLI"
     gene            2884..5466
                     /gene="alaS"
                     /locus_tag="VAH76_002768"
     CDS             2884..5466
                     /gene="alaS"
                     /locus_tag="VAH76_002768"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO1387353.1"
                     /translation="MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQ
                     FKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
                     SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHP
                     LPKPSVDTGMGLERLAAVIQGVHSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADH
                     IRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDA
                     YPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYG
                     FPIDLTADIIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGY
                     EKESMEAKIIGLLQEGNEVKSINKGAKGAVILDHTPFYAESGGQVGDKGLLIGKEFTF
                     QVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLHAALKKI
                     VGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIES
                     AKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIR
                     RIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEKVSQLLLDNKNQEKMIAKLLS
                     EKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQ
                     NKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEM
                     IEKI"
BASE COUNT         1823 a          871 c         1214 g         1661 t
ORIGIN      
        1 acgcaaatat ttcgtagagt ggatttatac aatgaaaatc tacggatcct cgaaagtgtg
       61 tttcttgttt taaactgaat aataaaagtt gcacaagaag tataataatt tatttgatta
      121 aaaaatagga aaaaatatga gtcaatatac aaggttgttt gagtttttat gtgatctgat
      181 tgatatccag attaaaaaat atgttgagct cgcaaaactt aaagtaggtg aagatgcgat
      241 aataaacgcc gctctcatat acgaagaggt gaatgaatta ttaggattcg cggagaagtt
      301 attaaaggaa atagaaacca attcaaaatc cgactcaatg gatatgacca tttttgatcg
      361 tgtttttgag caagtaaaat tttatgtgga gcaagaatat ctgagatcct atgcagaatg
      421 gctgcttatt gaaaataact ctaaagacac ggtctacaat agaacaaaag agcaattact
      481 caggctttca gaaattaaga gttttgatga taaaggtttt tcacaaccaa gcactgaaat
      541 tcaacagcaa tgtcaacatg atagtgttca gatagtttat tccattttaa atttggcaag
      601 agaggtaaga aaaaatccgg atatggggtt tgatgatagc atcccacata acaagctcaa
      661 tgtattaaga agaaatgcaa ctaccagata tgttgaattc ggaaatgaaa tagacaaatt
      721 gcatcagcat agtgaagaat ttttgcaaca gtcgagtctt ggagttttcg acgccgaatt
      781 agcctatgaa tcggcactat caaaggcaaa agaccatgat cttaaattaa atactttgtt
      841 tggtcgttat aaagaaaata aggaaaccgc ttctgttcct caatctctct ctttggtcaa
      901 taaggtagat gaggaaaata ttatgacaga aaaaaaacct tcaactgaag tgtctgataa
      961 taagacccct ttcttttcaa caagcaattt attatttttc ggtggtatag ttttagccgc
     1021 tgttattttt gttcccagat tgcttactca tctcaaggtc aataaattat gacacagcat
     1081 tatgagtagt taacattttc gttattttga ttgtaaaacc ttggttttta tgtcggattg
     1141 tctatatatt tttatattgc cccgttacca agctaaaaat caaggtttga agttcaaact
     1201 gacaacacct tcatttttga acaaatggtt attccacgta gcttataata aaagactaat
     1261 catcagctat cgtatgctac ttttatttag taatacacta agattactgt ttgctgcttg
     1321 ctcacatctg tgtgataatt ttgttcttgc acacattata atctggaata aattatggaa
     1381 gaaaataaac aaaaagcact atcagcggcg ctttcgcaaa ttgaacgcca atttggcaaa
     1441 gggtctgtaa tgcgtatggg agatagcacg gtatcacggg atattgaagc aatctctaca
     1501 ggttcattag gtcttgatat tgccttaggg ataggcggtt tgccaaaagg ccgtattgtt
     1561 gaaatttatg gccctgagtc ttctggtaaa accacgctca ctcttcaagt gatcgctgaa
     1621 tgtcaaaaaa tgggtggtac agcagcattt atcgatgcgg aacatgctct ggatccaagc
     1681 tatgctcaga aacttggtgt gaaggtggat gagctgttgg tttctcagcc ggatacaggc
     1741 gagcaagctc tggaaattac tgatatgctg gtgcgttctg cagcagttga tgtcgtaata
     1801 attgactcgg ttgctgcctt gactcccaaa gcagaaattg agggagaaat gggcgattcc
     1861 catgttggtt tacaggcaag attgatgtcg caagccctgc gtaaattgac cgctaacatt
     1921 aaacgttcga acacactggt tatttttatt aatcaaatac gtatgaaaat tggtgtgatg
     1981 tttggtagcc ctgaaactac tacagggggc aatgctttga agttttatgc ttcagttcgt
     2041 ttggatattc gccgtattgg ttcaattaaa aagggtgaag aaatattagg cagtgaaacc
     2101 cgagttaaag tagttaaaaa taaggtagcg ccaccgttta aaatgactga gtttgatatt
     2161 ttatataacg aaggtatatc tcgtgagagt gaaattatta atctgggtgt tcaattgaat
     2221 ttaattgaga aatcaggtgc ctggtatagc tacaaacagg aaaaaattgg gcagggcaaa
     2281 gaaaatgtca gattatattt gaaagaaaat ccccaagtcg ctgcagagct tgaacaacaa
     2341 atccgtactg agctcttgga aaaaaagtta tctgtgttgg ctagttcatc agaagatctt
     2401 tttgagacta tagatgacta aagcattcga tagcgccttg cgtctgttaa ccaggcgcga
     2461 gtatagcgct atggagttat gtgataaact gaagcagaaa ggatttagca ccaatgatgt
     2521 tcaaaatgct ttgtatgaat gccaacgtct tgggtatcaa agcgatgtgc gttttgtaga
     2581 aaattatatc agagtacgaa ttcatcaagg ctatggccct ttaaagatca ggcaggaatt
     2641 aaaaaacaag ggaatagatc cagatctcat tcaaagtgtg ctgcatcaag aaaaagacaa
     2701 ttgggtcaat tatgctttgc gggcatggga gaaaaaattt aagagacagg atgatttctc
     2761 ttatagtgaa atacaaaaac agcaacggtt tttgctgtat cgagggtttg atagggatgt
     2821 tatttcaaag gtatttaaag aggttaaatc ttcttactta atttaactgg taaatattaa
     2881 aaaatgaaaa gttctgaaat tagacaagca ttccttaact attttgttca acgcggtcat
     2941 caaatagtag cttctagctc attggtccct tcaaatgatc ccactttact atttaccaat
     3001 gcaggtatgg tgcaattcaa ggatcttttt ttaggtcttg aaactcgttc atatcagcgt
     3061 gctgctacag cacagcgctg tgtgcgtgca ggtggtaaac ataatgatct ggaaaatgtt
     3121 ggttatacgg cgagacatca tacatttttt gaaatgttgg gtaattttag ttttggtgat
     3181 tattttaaac gcgaagctat tcaatatgct tgggaattcc taacagaagt tttacacata
     3241 ccggcagaac ggctatgggt tactgtctat aaagaggatt tggaagcaga agacatatgg
     3301 ctaaaggaaa tgaaggtatc tcccgagcga ttctctcgat gtggggaaaa agataatttc
     3361 tggtcaatgg gggatacggg tccatgtggt ccttgcacag aaatttttta tgatcatggt
     3421 cctgaagtag caggtggtcc cccgggcagt ccagatgaag atggagatcg ttatatagaa
     3481 atatggaacc tggtattcat gcagtttaac agggacagag agggacattt gcaccccttg
     3541 ccaaagcctt ctgtggatac tggtatggga ttggaacgat tagccgcagt tattcaaggt
     3601 gtccatagta attatgaaat tgatagtttt cagtatttga ttaaagcaat agcacaattg
     3661 ggccaggata ttgatttaaa tcatacttcc ttaaaagtga ttgcagatca tattcgttcc
     3721 tgctcttttt tgatagttga tggggtattg cctagtaatg aaggcagagg atatgtatta
     3781 agaaggatta tccgcagagc agtcagacat gggaataaat taggcttacc cagcccattt
     3841 ttttccaaac ttgttcagcc tttgattgat gttatggggg atgcttatcc cgagctaatc
     3901 aattcgaaag cgcatattga aaggatcttg caacaagaag aaaatcagtt tacccgtact
     3961 ttggaacaag gattgcgttt attacaagat catattaaaa atttgcaagg ccaggaacta
     4021 tcaggagaag tggcattcaa gttgtatgat acttatggtt tccctatcga tttgacagca
     4081 gacatcattc gtgaacaagg tttacatatt gatatggaag catttaatca attgatgcaa
     4141 caacaaagag aacagtctca agccgccagt cagtttacta cagattatca cgccgtgtct
     4201 caattggatc atcagtctga atttcatggg tatgaaaaag aatccatgga agcaaagatt
     4261 attggattac ttcaagaagg taatgaagtg aaatctatta ataagggagc aaaaggggct
     4321 gtaattcttg atcatacccc attttatgct gaaagtggtg gtcaggtagg agataaagga
     4381 ttactcatag gaaaagaatt tacttttcaa gtcgatgata cacaaaaagt agggcaagct
     4441 gtcgtacatt atggaaaagt aattaaaggt gagctcactt tagatttatt aatccatgcg
     4501 caagtagata acataagacg ggatgcaatt cgattgaatc ataccgcaac tcatttgtta
     4561 catgctgctc ttaaaaaaat tgtagggcaa catgttcaac aacgaggatc cctggtggat
     4621 gctgaaaggg cacgttttga cttttctcat ttcgaggcat tgactcctca acaaattcag
     4681 caaattgaag aggtagttaa tgcacagatc cgagctaata atgaagtaat aactcaagta
     4741 atggacattg aatcagccaa gcaaagtggt gctgttgctt tattcgggga aaaatactcc
     4801 gacgcagtac gcgtattatc catgggtgat ttttccaagg agctttgtgg aggaactcat
     4861 gcacgacgta ctggtgatat aggtcttttc aaaatcgtag cagaatatgg tattgccagc
     4921 ggtatcaggc ggattgagat ggttacgggt cgttatgcat tagcctgggt caatgaacag
     4981 ctgggattta tgaataatct ggcagctacc cttaaaacaa cacctaatag tctacaggaa
     5041 aaagtttctc aattattgct tgataataaa aatcaagaaa aaatgattgc aaaactttta
     5101 agtgaaaaag cacaaaaatc tggagcagat attcttggtg agatcgagga gatcaagggt
     5161 attaatttat tgattaagca gttagagggt atggatagcc aaaccatgcg ccataccatg
     5221 gatcaattaa aatcaagaat tgattcagcg gtgatcatat tatttactat tgaacagaat
     5281 aaaatgaatg tcattgccgg ggtgagtaag aatattattg gcaaagcacc aagtgctgct
     5341 caattagtaa gacatctttg tggtaaagga ggtggtcgag atgatatggc tcaaggtggt
     5401 gggggtgttc ctgaagattt gaacagtaag atcaaagaaa taaaggaaat gattgaaaaa
     5461 atttaatatt tccagatttt tcttttcgac tgcagaaaag caagttgttt ttggatttgc
     5521 tataatatag acactggttt actaatgagc actatgatga atgacttgc
//
LOCUS       DATNSN010000186         5527 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.186, whole genome shotgun sequence.
ACCESSION   DATNSN010000186 DATNSN010000000
VERSION     DATNSN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.186"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            201..1091
                     /gene="ligD"
                     /locus_tag="VAH76_002769"
     CDS             201..1091
                     /gene="ligD"
                     /locus_tag="VAH76_002769"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEO1387354.1"
                     /translation="MMIEKVEITHPDKMIFSGAQISKSEVAEYYSRIANFILPWVNER
                     PVTLKCYPNGINKEGFFIKHAPDYFSDSIHRVSIPLKKTDAHIEMIRIDKAEDLVYLV
                     EQNTIELHIPLAKITDVKKPDQIVLDLDPSDDDFEKVRHAALLLKEILDLLQLPAFIK
                     TTGSRGVHIHIPIKPYYSFLEIKAITKQIAERLHQQSTDTTTLEQRKNKRGNKVFIDY
                     LRNDYGMTAVAPYSLRALENAPIATPIDWSELQDNALGPRSYHLKNIFRRLAQKQDPW
                     ANFVQSSVAIQCRDIGSNIF"
     gene            complement(1161..2090)
                     /locus_tag="VAH76_002770"
     CDS             complement(1161..2090)
                     /locus_tag="VAH76_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEO1387355.1"
                     /translation="MTITHTAAGGITMTVELIRNGETDTNDYEEHLDSFGYETLEKSL
                     KEDAKSICQFFCNEELVLQGLKKLVTQFYQFNQPEKKTLRQFFDDWGSKNHFNHEAPP
                     NDKIPYETSPNFHFPNHTPILHGELTADLFNNVLLKQGYLSADLGAGPKHGKWAHTIQ
                     FFILEEARKTGQLKLHSNTVCEFIKKISQIHGQYESFSLWNILFDSFDETFTCPNIIT
                     QTLTSPWDNSKEALFLADKLNHFQKKFEKAASTENNYNAYANKKYMSRIDKAHYVKYK
                     DKCAILWFAPRDEKSLPTTSKQEELKIEGLNQH"
     regulatory      2232..2335
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2322..2588
                     /locus_tag="VAH76_002771"
     CDS             2322..2588
                     /locus_tag="VAH76_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387356.1"
                     /translation="MSIVIFSGIVAFITSFIGLLPQIVKSLKTKSTEDLSMMMLVNYL
                     VCSLAWIIYGSSTNSFFVTSSNVVGLIVSFLLILLKRRYDARCN"
     gene            2572..3222
                     /locus_tag="VAH76_002772"
     CDS             2572..3222
                     /locus_tag="VAH76_002772"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO1387357.1"
                     /translation="MLDVINFKDYRSILCLNGDLPAPSFFHKRKLPVIAADGAANVLC
                     NLGVFPDLITGDLDSIQPALLENHSFLHLPDQGSTDYQKAMHYLKTHDLLPAIVVGIN
                     GGYLDHILNNINIFMDTNCLLYSPPIKGFVVNEKLRVNFILPVQTKISLIGIPEVVLS
                     SEGLKWELKHSHLSFPGKNSCFNRTQTAEISLEVHQGATLVLIYEDAIEDAGVSSL"
     gene            complement(3381..3830)
                     /gene="trmL"
                     /locus_tag="VAH76_002773"
     CDS             complement(3381..3830)
                     /gene="trmL"
                     /locus_tag="VAH76_002773"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEO1387358.1"
                     /translation="MIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQM
                     RRAGLDYHQWASILHYESWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPE
                     SCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM"
     gene            4108..5526
                     /locus_tag="VAH76_002774"
     CDS             4108..5526
                     /locus_tag="VAH76_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HEO1387359.1"
                     /translation="MDKNHNTKKILFKNRFVLLGFGCVGQALMPLIFEKFDIKPSQVT
                     IIAAEGARVEVAQKYGVSFKLQQITPQNYLEVIGSTLEENDFLIDVSIGISSLALIIL
                     CNQKGALYINAATEPWKEEFVMEKMALNRRTNYSLREEVLRLKDKTQKTALITHGANP
                     GLVSHFIKEALLNIAKDNGLTINRPQNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKS
                     PGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMV
                     RSWTPTLGAFHGFLITHAETISLTNFLTLKNGSELLYRPTVHYAYNPCPDARLSIFEL
                     KSNEWKPQNKNRLILNEIIDGCDELGVLLMGNQRGAYWYGSTLSIQEARQIAPYNNAT
                     SLQVVASMISGIIWAIEHPDEGIVEPEEVDHQYIIDIAKPYLGKVGGYYTDWTPLKNR
                     GELYPEEVDLSDPWQFFNIRVN"
BASE COUNT         1659 a          952 c         1086 g         1830 t
ORIGIN      
        1 ttttaatgag ggtaaaccaa atgactgaat taaaaccatt ttgtaataaa gaaattgggg
       61 gcagaagcat ctgtataaat aatttttatg aaaatttgac cagtaactca agcctgcatc
      121 atgacataat ttttcgattt aaaacgattt ttctgcttta ataaaattga aaagaaattg
      181 gaaattccag ggtactcgct atgatgattg aaaaggttga aattactcat cctgataaga
      241 tgatattttc tggcgcccaa atttcaaaaa gcgaggtagc tgagtattac agccgaatag
      301 caaattttat tttaccctgg gtaaatgaac gaccggtaac cttaaagtgt tatcctaatg
      361 gaatcaataa ggaaggtttt tttataaagc atgcgccaga ttatttttca gattccattc
      421 atcgtgtttc tatcccgctg aaaaaaactg atgcgcatat tgaaatgatc aggattgata
      481 aagcggaaga tctggtttat ctcgttgagc aaaatacaat agaattgcat atacccttag
      541 caaaaataac ggatgttaaa aaaccggatc aaattgttct ggatctggat ccgtcagatg
      601 atgattttga gaaggtacgg catgccgctt tattgttaaa agaaatcctc gatttgttac
      661 aattgcccgc atttataaaa acgaccggtt cacgtggtgt acatattcat attccgataa
      721 aaccttacta ttcttttctt gaaataaaag ccattacaaa acaaatagca gagcgattgc
      781 atcagcaatc cactgataca acgactctcg agcaacgcaa aaataaacgc ggtaacaagg
      841 tatttattga ctatttgaga aatgattatg gaatgacagc agttgcacct tattcactaa
      901 gagcacttga aaatgcacct atagcgactc caattgactg gagcgaattg caagacaatg
      961 ctcttggtcc ccgcagctat catttaaaaa atatatttcg tcgccttgca caaaaacaag
     1021 atccatgggc gaattttgtg caaagctctg ttgctattca atgccgggat attgggagca
     1081 atatttttta aaacatttgg acaagaatcc aaagaatatt aaaataaatc agttacttat
     1141 ctagtttgca tcaatttctg tcaatgttgg tttaatccct ctatttttaa ttcttcttgt
     1201 tttgaagttg ttggcagtga tttttcatcg cgtggtgcaa accatagtat tgcgcatttg
     1261 tctttgtact taacatagtg cgctttatca attctgctca tgtatttttt attggcgtac
     1321 gcattgtaat tattttcagt agatgctgct ttttcaaatt ttttttggaa atggtttaat
     1381 ttatcagcta aaaatagagc ctcttttgaa ttatcccatg gtgatgtcaa agtttgagta
     1441 atgatattag gacaggtaaa ggtttcgtca aatgaatcaa agagaatgtt ccataaactg
     1501 aatgattcgt attggccatg gatttgtgag atttttttaa tgaattcgca gactgtattg
     1561 ctgtgaagtt ttaattgtcc ggtttttctg gcttcttcaa gaataaaaaa ttgaatggta
     1621 tgcgcccatt taccgtgctt ggggcctgcg ccaagatcag cagataagta accctgtttt
     1681 agaagaacat tattgaataa gtctgcagtt aattcaccat gtaaaatcgg agtgtgatta
     1741 gggaaatgaa aattggggct ggtttcataa ggtatcttat cattaggcgg agcttcgtgg
     1801 ttaaaatgat ttttacttcc ccagtcatca aaaaattgtc ttaatgtttt tttctcaggt
     1861 tggttaaact gataaaattg tgtcaccaat ttttttaaac cttgtaaaac cagctcttca
     1921 ttacaaaaga attggcatat agacttggca tcttctttca aggatttttc taatgtttcg
     1981 tatccaaaag aatccaagtg ttcctcataa tcattggtat ctgtctctcc atttcgaata
     2041 agttctactg tcatagtgat gcctccagct gctgtatgag ttattgtcaa ataccgattt
     2101 gagaattatt tgaggggatt aattcctttt ttggactaaa ggattattaa ttaatcataa
     2161 aataatgaaa aaagaaagga gtattttctt gctattaaat tgcggtctgc tcatttcgtg
     2221 ataaaatata agtccaacat taggggtgcc aattgataag ttggctgaga ggatcaccca
     2281 accctttgaa cctgatctca ttagtctgag cggaggaaaa tgtgtcaatt gttatttttt
     2341 ctggaatagt tgcttttatt acctcgttta ttggactttt accgcaaatc gttaaatcct
     2401 taaaaaccaa atcaactgaa gatctttcca tgatgatgtt agtgaattat ctagtttgtt
     2461 cgctggcctg gatcatttat ggaagcagca caaactcatt ttttgtaacg agttccaatg
     2521 tagtgggttt gatagtgagt tttctgctga ttttattaaa aagacgttat gatgctcgat
     2581 gtaattaact ttaaagacta tcgttctatt ttatgtctta atggcgattt gcccgctcct
     2641 tccttttttc ataaaagaaa gctgccggtt attgctgctg atggtgccgc taatgtgtta
     2701 tgtaaccttg gtgtctttcc tgacttaatt actggagatc tggattctat tcagcctgct
     2761 ttgttggaaa accattcttt tcttcattta ccagatcaag ggagtacgga ttatcaaaag
     2821 gccatgcatt atttaaaaac tcatgatcta ttgcctgcta tcgttgtagg gataaatggt
     2881 gggtatttgg atcacattct gaataacatc aacattttta tggatacaaa ctgcctttta
     2941 tattcgcctc ccatcaaagg ttttgttgtc aatgaaaaat tgagggttaa ttttatttta
     3001 cctgtccaga ctaaaatctc gttaataggt atccctgagg ttgtcctgtc ttctgaaggt
     3061 ttgaagtggg agctaaagca ctcacacctt tcttttcctg ggaaaaattc ctgtttcaat
     3121 cgaacacaaa cagcagaaat tagtttggag gttcaccagg gcgccaccct tgttttgatt
     3181 tatgaagatg ctattgagga tgctggagtt tcctctttat aaggaataag gacttacgct
     3241 ctcctcaatt tctttgttaa aaaatatttt tgcctcggcc ttgtggtttt tcgtaagtcc
     3301 tgtttatatt agagtactgc ttgcataacc tgtctctttc attgctgtaa tcgctatcag
     3361 gtggattacc aggttaatgc ttacatgttg aaatccattt gcctccatgc ttcgtacaat
     3421 atgattgcta cagcatttga taagttgaga ctacgattat tttctctcat gggaatccgt
     3481 attgttgaat attggttcag tatttgttga ggtaagccgc aagattcggg tccaaataat
     3541 aggatgtcat ctgccttata atcgacgtca ctatacttca cttttccttt tgcgctacag
     3601 caaaataatc gtcgttgtga atgagactgc aagaatgttt gccaactctc ataatgcaat
     3661 atggaggccc attgatgata atcaagacct gctcgtcgca tttgtttatc ttccaaagta
     3721 aagcctaatg ggtgaatcaa atgcagttga gctccactat tggcgcagag tcgaataata
     3781 ttgcccgtgt tcggtggaat ttctggttga taaagcgcaa tatggatcat gtgattttcc
     3841 agtagcattc tggctatcat tttaattgat ttattcgaag ccaggaatag agtggcagga
     3901 tgcaatttta ctacatactt taaaaatcga gcagggagtt gatatgtcta attttttact
     3961 caacaggctg tctgaattca acatagctac tacaatatta gtatgctata tgaaagttat
     4021 tgatgtctgt tgggtatgat cattgttgaa tagttgttga gtattaaaaa ttcaaattaa
     4081 actgggccgg gaagttaagg attcatcatg gataagaatc ataatacaaa aaaaattttg
     4141 tttaaaaatc gttttgtctt actaggattt ggttgcgttg gtcaggcatt aatgccttta
     4201 atctttgaga aatttgatat caaaccatcg caagtgacaa tcattgccgc tgaaggggca
     4261 agggtcgagg ttgctcaaaa atacggagtt tcattcaagt tacaacaaat tactccccaa
     4321 aattatcttg aagtgattgg tagtactttg gaagaaaacg attttttaat tgatgtgtca
     4381 atcggcatat caagtcttgc tctgattata ctatgtaatc aaaaaggagc attgtatatt
     4441 aatgctgcaa ctgagccgtg gaaagaagaa tttgttatgg agaagatggc gcttaatcgc
     4501 cgaaccaatt attctttgcg tgaagaagtc cttcgcttaa aggataaaac tcaaaaaaca
     4561 gccttaatta ctcacggggc taatccgggt ctcgtttcac attttattaa agaggccttg
     4621 ttgaatattg caaaggataa tggccttacc attaacaggc ctcaaaatgc agcagaatgg
     4681 gctaatcttg ccatgacttt gggaattaaa gtaatccatg ttgcagagca agactctcaa
     4741 gttacctatc ctccaaaatc accaggcgaa ttcgttaata cctggtccgc taatggtctg
     4801 attttggaag gactgcaacc tgctgaaatc gggtggggga cacatgaagc tcattggccc
     4861 catgacgctt attctcatag taatggacca caatgcgcga tctatctgtc tcgccccagt
     4921 gccggtgtaa tggtgcgttc atggacgcca acactgggtg cttttcacgg ttttttaatt
     4981 actcatgcag aaactatttc acttaccaat tttttaacat taaaaaatgg atctgaatta
     5041 ttgtataggc caacagttca ctatgcctat aatccttgtc cagatgccag gctctccatt
     5101 ttcgaattaa aaagtaatga atggaaacca caaaacaaga atcgcctgat tttaaacgaa
     5161 atcattgatg gatgtgatga attgggtgtt ttgttgatgg gtaatcaaag aggggcgtat
     5221 tggtatggtt caaccctttc tatccaggaa gcaaggcaaa ttgcccccta taataacgca
     5281 accagtttac aagttgtcgc cagtatgatt tcaggaataa tctgggcaat agagcatcct
     5341 gatgagggta tcgtagaacc agaggaagtt gatcaccaat atattattga tattgccaaa
     5401 ccgtatcttg gaaaagtcgg cggctattac acggattgga cccccttaaa aaatagaggg
     5461 gaactttatc ctgaagaagt ggacttatct gatccatggc aatttttcaa tataagggtt
     5521 aattaaa
//
LOCUS       DATNSN010000187         5275 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.187, whole genome shotgun sequence.
ACCESSION   DATNSN010000187 DATNSN010000000
VERSION     DATNSN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.187"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(301..1296)
                     /locus_tag="VAH76_002775"
     CDS             complement(301..1296)
                     /locus_tag="VAH76_002775"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEO1387360.1"
                     /translation="MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQL
                     NDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSKGFSQPFNLHLFTSIDSTNRYLK
                     DLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
                     LATVLTINEFIVSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINS
                     DTKNNPLPDKSWCSFFGITNQNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKY
                     DYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHELSSGDTSLALSNFE"
     gene            complement(1300..2037)
                     /locus_tag="VAH76_002776"
     CDS             complement(1300..2037)
                     /locus_tag="VAH76_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEO1387361.1"
                     /translation="MIITGFTPLNTQNCILNESRIDLWQFSLDYELHSAYQLLNADER
                     SRADRFYFNRHKRRFTNARATLRIILARYLNTPPERLEFTYNAHGKPNVINSQKLQFN
                     ISHTGEMAILAVGKTYPIGVDIERYSARPYEGIGKNLFSEQEYQELKKAHQSLKPALF
                     FHIWAQKEAFIKASGLGLSYPTKEFHVPTSFPTKQLVDDPLHNMTWQLRSFMPEVCCS
                     GALCYHPSVREIRHGIVQLQQNISLKF"
     gene            2119..3072
                     /locus_tag="VAH76_002777"
     CDS             2119..3072
                     /locus_tag="VAH76_002777"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEO1387362.1"
                     /translation="MTRQFLEFEQPIEELNQKIEALRMVGSDNEVNLSEEIARLEAKC
                     SELTENIFSRLEPWQIAQMARHPLRPQTTDYIERIFTDFQELHGDRSYSSAPAIIGGM
                     ARLNGEPVMVLGHQKGKRTKEKVYRNFGMARPEEYRKALRLMRMAEKFKMPVVTFIDT
                     AGAYPGIGAEERNQSEAIARNLFAMSRIKTPIVCIVTGEAGSGGALAIGVGDKIIMLQ
                     FSIYSVISPEGCASILWKDASKASEAARAMGITADRIFENELVDMVVPEPLGGAHRDV
                     DEMASRLKRLLTSELQALKKVPLDQLIELRYQKFMAMGACD"
     gene            3065..4360
                     /gene="tilS"
                     /locus_tag="VAH76_002778"
     CDS             3065..4360
                     /gene="tilS"
                     /locus_tag="VAH76_002778"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO1387363.1"
                     /translation="MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLS
                     NQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDF
                     FSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
                     PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHC
                     QQAKANLEVLAIKDCPDLLNATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKT
                     FHRIIHEIIFAKLDAMPTVSWDQIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTY
                     PDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVR
                     DRVPLVYINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS"
     gene            4623..5159
                     /locus_tag="VAH76_002779"
     CDS             4623..5159
                     /locus_tag="VAH76_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387364.1"
                     /translation="MISESVIPLAYFITFTCYGTWLHGGKSTSVDRYNNIPGTEFIPY
                     DRRRLYQVKNLMLETPYSLDELRRHIVLQAICEVCRYRQWVLLAAHVRSNHVHLVIHA
                     QISPEHIMNTVKAYASRYLNYTRLDGDRKNRWTRHGSTRYLWNEEEIEATIQYVVYEQ
                     GLPMAVFENKDRVFGVEV"
BASE COUNT         1593 a          961 c         1090 g         1631 t
ORIGIN      
        1 tacaaaaaat acgtcaaccc ctaaaaagcc agcgggtacg aaattgatat gtagatgatc
       61 aaaaattact agtaaaacag caactcctct taatgcatca atatcttttc tatagtttat
      121 tctggcagta tttgacatta agtttaaacc ctatcaacag atgacgcttt attagattga
      181 aaaatgaaca ttaaccttga ttttgtacat gttgggtgaa aagcgattat agaataaagt
      241 gaagttatta aaatattaga tttgaaattt tactctaaaa cttccttttt gttaaaaacc
      301 ctattcaaaa ttacttaggg caagtgatgt atcacccgaa gagagttcat gcttatttcc
      361 aaattcatct tccagaatca gttggccaaa agaattaatg cccaaggcaa tcccggaaat
      421 cgaattcaaa gcttgggaca ctttaacttt tttcccaaca aggtagtcat acttattcca
      481 gtcatcctta aatgcattta gatcgttatt aataaatttt gatagatatt tttccaggtt
      541 aattaatagc cttgtaacaa tcgaattccg attgtaattt tgattagtta ttccaaaaaa
      601 agaacaccag gatttatctg gtagaggatt gttttttgta tcagagttga tatttaaacc
      661 aacacctata ataaattgtg ctgtcgcgtt agattctgca ataatctcta ctaaaatccc
      721 acataacttt ttattattcc acaggatatc atttggccat tttattttta ctttatcaga
      781 tacaatgaat tcatttatag tgagaactgt tgctaaactt atgactaaac tgagacccga
      841 tagcttagat atgtcacaat taaaactcca ccgggtagag cagtaaatat tctctccaaa
      901 aggagagtgc cagtgtcgtc caaagcgccc tctcccttga gtttgcattt cagtgcagca
      961 aatttcgacg gctgagcttg aaggcagatc tttcaggtag cggttggttg agtcaatcga
     1021 tgtaaataaa tgcaggttaa acggttggct aaatcctttg ctgtgtaatt gcttggtaat
     1081 ttcattggag tctagcagga ttaaaggctt ttccaattgg tatccttgat gtggtatgcg
     1141 gataataggt atgcctaaat cattaagctg attgatttgt ttccatactg ctgagcgact
     1201 gatttttaaa gcgtttccta attcactccc actatgacat gccccatcac ccagtatttg
     1261 cattaacgta tgttgagaag tattaaattg aaacatgatt taaaatttta aactaatatt
     1321 ttgttgaagc tggacgatcc catgacgaat ttctctaaca gaagggtgat agcataaagc
     1381 gccactgcag catacttccg gcatgaatga gcgtagttgc caggtcatgt tatgcaaggg
     1441 atcgtcgacc aattgttttg ttgggaaact ggtagggaca tgaaattctt tggttggata
     1501 ggagagacct aaaccactag ctttgatgaa cgcttccttt tgagcccata tatgaaaaaa
     1561 taaggctggc ttaagggact gatgtgcttt tttcagttcc tggtattctt gctcggaaaa
     1621 caaattttta ccaatccctt cataaggccg ggctgaatat ctttcaatat ccactccaat
     1681 cggataggtt ttacccactg ccaatatggc catttcacca gtatgactga tattaaattg
     1741 caatttttgc gaattaatca cattgggctt gccatgtgca ttgtaagtaa attccaatcg
     1801 ttcagggggc gtattaaggt atcgggccag gataattctt aaagttgctc tggcgttggt
     1861 aaagcgtctt ttatggcgat taaaataaaa gcgatcagca cgggagcgtt catcggcatt
     1921 caataactga tacgcactat gcaattcata gtctaatgag aactgccaaa gatcaatacg
     1981 tgactcattt aaaatgcagt tttgtgtgtt taatggagta aatcctgtaa ttatcatctg
     2041 ttgtatctga aatctggcat cgattaacgg ttaagagtat catataccaa agatgtttaa
     2101 ttcaccaaag agtaatcaat gacccgacaa tttttggaat ttgaacaacc tattgaagag
     2161 ttgaatcaaa aaattgaagc actgcgtatg gttggcagtg ataatgaagt taatttaagt
     2221 gaagagatag ctcgacttga agcgaaatgc tccgagttaa cagagaatat attctccagg
     2281 cttgaaccat ggcaaatcgc tcagatggcc agacatcctt taaggccgca gactactgac
     2341 tatattgagc gaatttttac tgattttcag gagttgcatg gtgatcgcag ttattcttca
     2401 gcccctgcaa ttatcggagg catggcgcga ttaaacgggg agcctgtcat ggtgcttggt
     2461 caccaaaaag gtaaacgtac caaggaaaaa gtatacagaa actttggtat ggcaagacct
     2521 gaagaataca ggaaagcact gcgtttgatg agaatggctg aaaaattcaa aatgcctgta
     2581 gtcacattta ttgatacggc tggagcttat cctggcatag gtgctgaaga acgtaaccaa
     2641 tcagaagcca ttgcacgcaa tctttttgct atgtctcgca ttaaaacacc aattgtctgt
     2701 attgttacag gagaagccgg ttcaggtggt gccttggcga ttggggtcgg tgataagatt
     2761 attatgctgc aattcagcat ttattcagtc atttctcctg aggggtgcgc ctcaatcctt
     2821 tggaaagacg cctctaaagc gagtgaagcc gcgcgtgcta tgggaattac tgcagacaga
     2881 atattcgaaa atgaattggt tgatatggta gtccctgagc ctttgggggg cgcacaccga
     2941 gacgttgatg agatggcaag tcgcttgaag agattattaa cgagtgaact ccaggctttg
     3001 aaaaaggtgc ctctggatca attaattgag ttaaggtatc aaaagtttat ggcaatgggt
     3061 gcttgtgact aggcctttac taagtcccga atgggtagct cgccttaaac aattcaagaa
     3121 attgatagta gggttcagcg gtggtctgga ctctacagtg ctcctgcatg tccttgcctc
     3181 aactcctccc ttgtctaatc aacttttggc tgtccatatt aaccatggca ttagtggaaa
     3241 ttctctgaac tggcaaaggc attgtgagca attgtgcctg gatttaggta ttgcttttat
     3301 tgccaaagcg attgaatttg atcgttctgc aaatgttgaa gaagcagcac gcaatgcgag
     3361 atatgatttt ttttcctcct tattagagga taatgattgt ctggttttgg ggcatcatct
     3421 tgatgatcag gctgaaacag ttcttttgca actgtttcgc ggtgcaggag ttgatggctt
     3481 ggcagccatg caggaatgca gcaatttggg tgctgggcaa ttggcaagac catttttgac
     3541 ctgccccaga gtggaattag agcattatgc tcgtattcat gaattgaaat ggatagaaga
     3601 cgaaagcaat caagatacaa aatattctcg caattatctg cgccatcgcg taatgccatt
     3661 attgcttgaa aagtggccgg gagtagcagg taatatttcc cgagctgcga ctcattgtca
     3721 gcaggcaaaa gcgaatcttg aggtattggc tataaaggat tgtcctgatt tgttaaacgc
     3781 tactgatcat ttgctgattg aacccataaa aggacttgaa tttgagcgga ttacaaatgt
     3841 tttaaagttt tggcttaaaa aaaatagaat tcaattgcct tcgtccaaga cgtttcatcg
     3901 tatcattcat gaaataattt ttgcaaagct cgatgccatg ccaacagtga gttgggatca
     3961 aatacaagta cgtcgtttcc aacaacatct ttatttgctt aaggcagacc agatcaattt
     4021 accgaaggct attaaatggc aacaatttcc agcttcttta acttatccag acgctgatat
     4081 taagttgtct gcagtaaagg cccaaaaggg attaatgatc ccgaaagatg cgcaaataga
     4141 aatacgattc agaaagggtg gtgaagagat ttattggcat ggtcaaacca aacatctaaa
     4201 aaaactattt caggaatggc aaattccccc atgggtaagg gatagagttc cattggttta
     4261 cattaatgat caattggctt gtgtagtagg ttatgcagtg agcgatttgt tttttactac
     4321 taatccactg gaagcttggt ctatagttaa taattcttaa tataacaatc tccgctccct
     4381 tacggtcgcg gctcggtttt agccataatt tcatttaatc cactccctta tggccgcggt
     4441 tcggttttag ccataattcc atttaataat ctgattaatc atctgagccg cgaccgttag
     4501 gaagcggaaa actggttatt ttactaattt tcgaaagcaa aatttacttc aaaaataatt
     4561 agttagtgtg ataacctttt cttacataaa caagtcctaa accaaatgga aatggtgaaa
     4621 taatgatatc tgaatcagtc attccactcg cctattttat tacctttacc tgttatggaa
     4681 catggttaca tggaggcaaa tcaacttcag ttgatcgcta caataatatc ccaggaactg
     4741 aatttatccc ttatgatcga aggcggttat atcaggtaaa aaacctcatg ctggaaacac
     4801 cttattccct ggatgaatta aggcgtcata ttgttttgca agcaatttgt gaagtgtgca
     4861 gatatcgtca atgggtatta ctggctgctc atgtaagatc aaatcatgta catcttgtta
     4921 tccacgccca aatttctccc gaacacatta tgaatacagt aaaagcatat gcttcacgtt
     4981 atttaaatta tacaaggctg gatggtgata gaaagaacag atggactcgc catggaagta
     5041 ctcgctatct ttggaatgag gaagaaattg aagcaacgat tcaatacgtt gtttatgaac
     5101 aagggttacc aatggctgtt tttgaaaata aagacagagt gtttggtgtt gaagtttgat
     5161 ctatccgctt tcggttttag ttataattcc atttaatcca ctcccttacg gtcgtggctc
     5221 ggttttagtc ataattccat ttaattatct aattaatcat ccgagccgcg actgt
//
LOCUS       DATNSN010000188         5256 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.188, whole genome shotgun sequence.
ACCESSION   DATNSN010000188 DATNSN010000000
VERSION     DATNSN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.188"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            139..2784
                     /locus_tag="VAH76_002780"
     CDS             139..2784
                     /locus_tag="VAH76_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387365.1"
                     /translation="MDVGQKIRILKRYLKEKSVTPEDVNLNDQDLTAYARLDKLLDYI
                     SIHNNGLFDFTTLESDEKQWKQYVDILNYSATYSPGSPPALIDTSERKAQFGLQYWLN
                     TRTIEALPRDNYYTRTVTLSEEADEELEQLITESRAKLYEITQTFTEVSSFNPANIIQ
                     VTNQISSIKEKLDTLTGNNPQITRLQKKLNVLTDLNKINQLERLNIPQDNGSNLSYCT
                     LAETIEGVTEKIPLITLAKVKEQLRHLCAQNELDLDSYSVSREQYYKLGIEGILKLPK
                     YGDIKEVQREAMALNISRLMGLDTTSSTTVSYNGHPALFIPFDNINLLNEFSSGKIFK
                     VGMGLSGQSYTHYSTIKPLGEGIQADLYIKDFGHALALLYLCCDTDAIGGYCKNKALR
                     NYSSLYIFDQVITDTDKFILDSRISLQPDQFIMKHTRHGQGRNRTLIEDSSLVTKYAS
                     LVRLKELKRIITQYCDHISWLHHNRTQAIKKQLQEELAKDERVRLTEELNDVEILEKD
                     AEQIKSQILIRINKIAEVLPQTTGPVGSDVIRQCLILEKLLHNPVLYSDDGRPYKNPW
                     TTRQANTVQTINDLQNGKIQITFKFNIPEEMVDFIKRHGGGDSLTISSPKVITISKAH
                     LDGLRENMLHPEHSPELSLNTDYLDDKDLCIIKMAYRIGNRTKILNAIAEYRVLMNNE
                     QVSFHNKIECLAQTEAKIKALIELANDKGLGMHILKKFYFDAQQRLQKLINPEVLPHD
                     IGQAFAAALRLDRVSELNKVVREAIVQNKVTDPQFVTFLTNCIKMETLATNYKDAQKA
                     SRALLIESQRIINHLKSPVVPLIVQLGGQVHSQAELDTDGLANLENDLQLDFEKPDTE
                     SMITMDLHQAVTKEEMIGNGMTVKV"
     gene            2860..3417
                     /locus_tag="VAH76_002781"
     CDS             2860..3417
                     /locus_tag="VAH76_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEO1387366.1"
                     /translation="MPVHERRQHFRIDDHIYFDYRILAPGEFCSDIEITNQLLGENGQ
                     KYMEAAQYFQGIDYELAELTQVMAVKEPALAHYLNLLNAKIDYLSRQMLMSSKVQLRK
                     VNISLGGMAFKTMEQIKEKTNLKIILYTKPKMIPIIVDAVVVYSQYQSETHYRTAVAF
                     CNLTSEQEQLLSQHILQAQIKSRAD"
     gene            3493..3762
                     /locus_tag="VAH76_002782"
     CDS             3493..3762
                     /locus_tag="VAH76_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEO1387367.1"
                     /translation="MSRTVFCCKLKQEAEGLEKQPFPGELGKKVFNEVSKQAWNMWLS
                     HQTMLINEYRLNLIEARAREFLKEEMQKYFFGEGSEKPSGYKEIK"
     gene            3774..4700
                     /locus_tag="VAH76_002783"
     CDS             3774..4700
                     /locus_tag="VAH76_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEO1387368.1"
                     /translation="MFHDLLEFNEEVLDAINDKNPIVALESTIISHGMPYPDNLTTAI
                     EVENIIRRQGAIPATIAMHQGKIRIGLTQEVMEHLALQKEVIKASRRDISFVLSRKVT
                     ASTTVAATMFCAHMARLPLFVTGGIGGVHQDVTMSFDISADLIELSNTPVTVVCSGAK
                     SILDLPKTLEVLETFGVPVIGYATDEFPAFYSRSSGIPVPQRLNSVEEVANLMSIQQK
                     LNMKNGIVVANPIPVSAELSDEEISPYIKQAHEEAKHMSGKSLTPFLLKRIVELTAGK
                     SLEANIELIKNNAFLGAEIAIAYQKKLFSKKT"
     gene            4751..4826
                     /locus_tag="VAH76_002784"
     tRNA            4751..4826
                     /locus_tag="VAH76_002784"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4784..4786,aa:Phe,seq:gaa)
     gene            4920..5168
                     /locus_tag="VAH76_002785"
     CDS             4920..5168
                     /locus_tag="VAH76_002785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387369.1"
                     /translation="MNIKFTDYREKIIQELNNKKVFLGINEPVSLIDGFINQPIQQEL
                     SGNIVIGGPTIPMVAVVGNNSGRIYYFALKALLPSIGI"
BASE COUNT         1859 a          959 c          984 g         1454 t
ORIGIN      
        1 atcagagtca aaataatgac ttatttttga ctgggaagta attaaaaata actaaaaatt
       61 tgaaggaaaa cattgaatag aaaaagcatg tttataaata tggaaataag tctaattgat
      121 actataagga aatagacaat ggacgttggc caaaagataa ggattttaaa acgttattta
      181 aaagaaaaaa gtgtcacgcc agaggacgtc aatctcaatg atcaagatct aacagcctat
      241 gccaggctgg ataagctatt ggattatatt tctatacata acaatggatt gtttgacttc
      301 acaacattgg aatctgatga aaagcaatgg aaacaatatg tggatatact taactattcc
      361 gcaacctact caccaggctc tccccctgct ttaatcgata ccagtgaacg caaagctcaa
      421 tttggtttgc aatattggct aaatacgaga acgattgaag cactgccaag agacaactat
      481 tatactcgta cagtcacctt atccgaagaa gcagatgaag aacttgaaca gctgattaca
      541 gaaagcagag ccaaattgta tgagataacg caaactttta cagaagtcag ttcttttaat
      601 ccagcaaata ttatccaggt aaccaaccaa atctcttcga ttaaagaaaa actggatact
      661 ctgacaggca ataatcctca aataacacgt ttacaaaaaa aactcaacgt actaacagat
      721 ttaaacaaaa ttaatcaact cgagaggctc aatattcccc aagacaatgg ttctaacttg
      781 agttattgta cgctcgcaga gactattgaa ggagttactg agaaaatacc tttaataaca
      841 cttgccaaag tgaaagagca attgagacat ctttgcgctc aaaatgagct tgatctggat
      901 agttatagtg tgtcaagaga acaatattat aaacttggta tagaaggtat actcaaatta
      961 cccaaatatg gcgacataaa agaagtacaa cgtgaagcca tggctcttaa catctcaaga
     1021 ttaatgggtt tggatactac ttcttcaaca acagtaagct ataatggtca tcccgccttg
     1081 tttattcctt ttgacaacat caacctattg aatgaatttt cttcaggtaa aatttttaaa
     1141 gtaggcatgg gattaagcgg gcaaagttat acccattact ccacaattaa accattaggt
     1201 gaaggaatcc aggccgatct ttatatcaag gattttggtc atgctctggc attactttat
     1261 ttatgttgtg atacagatgc tataggtggg tattgtaaaa acaaagcatt aagaaactac
     1321 agtagcctct atatttttga tcaggtcatt acggacactg ataaatttat ccttgattca
     1381 cgaattagtc ttcaacccga tcagttcatt atgaaacata cccgtcatgg gcaagggaga
     1441 aacagaacct taatcgagga ttcttcccta gttaccaaat acgcaagcct cgtgcgactt
     1501 aaagagttaa aacgcattat tacccaatac tgtgatcata tttcatggct gcatcataat
     1561 cgtactcaag ccattaaaaa acaattgcaa gaagaacttg caaaagacga acgcgtacga
     1621 ttaacagaag agttaaatga tgttgaaatt ttggaaaaag atgctgagca aataaaaagc
     1681 caaattctaa ttcgcatcaa taaaatagcc gaagtgctac ctcaaacgac tgggcccgtt
     1741 ggctccgatg tcatccggca atgtttaatt ctggagaaat tattacacaa cccggttctc
     1801 tactctgatg atggaagacc ttataaaaat ccatggacga cacgacaagc gaatacagta
     1861 caaacgatta atgatttaca aaacgggaag atacaaatta cttttaaatt caatattcca
     1921 gaagaaatgg tcgactttat caaacgccat ggtggtggag attccctgac tattagctct
     1981 cctaaagtga taacgattag caaagcccat ctggatggcc ttagggaaaa tatgttgcat
     2041 cctgaacatt cacctgaatt gtctcttaat actgattatt tagatgataa agacctctgt
     2101 attataaaaa tggcttatcg tataggtaat agaaccaaaa ttttaaatgc tattgcagaa
     2161 taccgcgtcc ttatgaataa tgaacaggta agctttcata acaaaataga atgtcttgcc
     2221 caaactgaag cgaaaatcaa agcgctgata gaattggcaa atgacaaagg cttggggatg
     2281 cacattctta aaaaattcta ttttgatgcg caacaacgac tccaaaaatt aattaatcca
     2341 gaagtgttgc cccatgacat tggtcaagcc tttgccgcag ctcttagact ggacagagta
     2401 tcagaactta acaaggttgt gagagaagct attgtccaaa ataaagtgac cgaccctcaa
     2461 ttcgtaactt ttttgacaaa ctgcatcaaa atggaaactc ttgccacaaa ttataaagac
     2521 gcacaaaaag caagcagagc cttattaatc gagtctcagc gaattataaa tcatctaaaa
     2581 tcgcctgtag ttcctttaat tgttcaatta ggagggcaag ttcattcaca ggctgaatta
     2641 gatacggatg ggttagccaa tcttgaaaat gacctacagc ttgattttga gaaaccagat
     2701 acagaatcaa tgatcactat ggatttacat caggctgtta cgaaagaaga aatgattgga
     2761 aatggaatga cagtaaaagt ttaatagatt tcttttaaaa ctatctgtaa tgcagcaaat
     2821 gaaaagagag atgtttaaac ttggaataat ggagtgacta tgccagtaca tgaaaggcgt
     2881 caacattttc gtattgatga tcatatctat tttgattatc gaattcttgc accgggtgaa
     2941 ttttgttccg atatagaaat cactaatcag ttactaggtg aaaatgggca aaagtacatg
     3001 gaagcagcgc aatattttca gggaattgat tatgagcttg ccgagctaac tcaggtaatg
     3061 gcggtgaaag aacctgcttt ggcgcattac cttaatcttt tgaatgccaa aattgattat
     3121 ttatctcgac aaatgttaat gtcgagtaaa gttcaactac gtaaagtcaa catcagcctg
     3181 ggcggcatgg catttaaaac aatggagcag attaaagaaa aaaccaatct aaaaattatt
     3241 ctgtatacaa aacccaaaat gatacccatt attgttgatg ccgtagtggt ttatagtcaa
     3301 tatcaaagtg agactcatta ccgaacagcg gtggctttct gtaatttgac cagtgagcaa
     3361 gagcagttgc tttcacaaca tattttacaa gcccagataa aaagccgtgc tgactaagat
     3421 ggagatgctg tagattggaa ctgttttttc taggataatg agccttcaaa ttaaaataac
     3481 gaggttatat ccatgagcag aacagtattt tgctgcaaat taaagcaaga agcagaaggc
     3541 ttggaaaaac aacccttccc tggtgaattg ggtaagaaag tttttaatga agtatcaaag
     3601 caagcatgga atatgtggct ttcccatcaa accatgttaa tcaacgaata ccgcctgaat
     3661 ttgattgaag ccagggctcg tgagtttttg aaagaagaaa tgcaaaaata tttttttggc
     3721 gaaggttcag aaaaaccctc tggctataaa gaaataaaat aaaggattaa tttatgtttc
     3781 atgacttgtt agaatttaac gaagaagtac tggatgctat caatgataaa aacccaattg
     3841 ttgctcttga gtcgaccatt atctctcatg gaatgcccta tcctgataat ttaactacag
     3901 caatagaagt tgaaaatatt attcgtagac aaggcgccat tccagctact atagcgatgc
     3961 accaagggaa aatccgtatt ggtttaactc aagaagttat ggagcatctc gcgctgcaaa
     4021 aagaagtaat aaaagcctct cgcagagaca tttcttttgt attatctcgt aaagtgactg
     4081 ccagcacaac ggtagcagca accatgttct gtgcccatat ggctaggctc cccctatttg
     4141 tcaccggagg tataggtggt gttcatcaag acgtgacaat gagttttgat atttcagctg
     4201 atcttataga actatccaat acgccagtta ccgtagtctg ttctggcgca aaatcgattc
     4261 tcgacttacc aaaaacattg gaagttcttg aaacgtttgg ggtgccggtc ataggttatg
     4321 ccacagacga attccctgct ttttacagca gatcgagtgg aataccagtc ccacaacgct
     4381 taaactcggt cgaggaagta gcaaatctca tgtcaattca acagaaattg aacatgaaaa
     4441 acgggatagt cgtcgcaaac cccattccag tatcagcgga actatctgat gaggaaatca
     4501 gtccatatat aaaacaggct catgaagaag ccaaacacat gagcggaaaa tcattaaccc
     4561 ccttcctgtt aaaacgtatt gttgaattaa ctgcaggcaa gagcctggaa gctaatattg
     4621 agcttatcaa aaacaacgct tttctaggag cagagattgc catagcctac caaaaaaaat
     4681 tattttcaaa aaaaacttaa tcaacacttg actcaaagtc gatctcggag tttaatagca
     4741 agccttgagt ggccagatag ctcagtcggt agagcagagg actgaaaatc ctcgtgtcgg
     4801 cagttcgatt ctgcctctgg ccaccattag aatcaatgag ttacgtaaaa atccaaaaaa
     4861 ttcaaatttt ccatgtagac gcaatgtaga caacttatta tcgggtcatt ctatttccta
     4921 tgaatattaa atttactgat tacagggaaa aaatcataca agaacttaac aataaaaaag
     4981 tttttttggg aattaatgag cctgtaagtt taatagatgg ttttataaac caacctattc
     5041 agcaagaatt gtcaggaaat atagttatcg gaggtcctac tatccctatg gtagcagttg
     5101 taggaaataa tagtggaaga atttattatt ttgctctaaa agctttactt cctagcatag
     5161 gtatataact tatgtcatca aatccagatc ctaactttaa agtagaatca actaaaactg
     5221 aggttatttc tagctccaaa gagcttccac agctaa
//
LOCUS       DATNSN010000189         5249 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.189, whole genome shotgun sequence.
ACCESSION   DATNSN010000189 DATNSN010000000
VERSION     DATNSN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.189"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            11..883
                     /locus_tag="VAH76_002786"
     CDS             11..883
                     /locus_tag="VAH76_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEO1387370.1"
                     /translation="MLDASLRDGGHRTNFHFKDDDLKQILLPLDNSGIEYIEIGYRNG
                     SLHPIENIGKAGMCPQDYLLYCQSLIKKAKIAVMVHPHNVNEQDLLELKNCGVKLIRI
                     CIAKGDLVKAVPLIKLAKNLNFETSVNIIHISYYTESELDSLIEEVSKHEPDMIYFAD
                     SNGSLFPDRINCLYKKYTRQYKVSFGFHAHDNLGLAQANALAAINAGAQFIDASLAGM
                     GKGTGNLKTEFFIAYLHANNIKKYNLENVLTAANYVRDALKIGQEPIEMSEFIRGISD
                     LSTADLKSYKPGKL"
     gene            1022..1729
                     /locus_tag="VAH76_002787"
     CDS             1022..1729
                     /locus_tag="VAH76_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387371.1"
                     /translation="MAKKRLLIIGPVSTQSSSLRHLSQNLSFLNPYYSIECLDPLVTL
                     QECPDEEYYGIWQNQLKPLIKIYDAFVGFSFGGVILQQCFSIFSEEKKQIILFSTPTF
                     ADSILREKLETVISLCSQNKLEEGLSSLYQAVYAPHSIPDASLQYLNRELAIKRLIFG
                     LTKVLKTNCVSILNHCNVNHLHLIGEKSQLVNTNNVIKPSCGQLIVVPEASMRVLESN
                     PTFCKEAILKALNHEIH"
     gene            1716..3560
                     /locus_tag="VAH76_002788"
     CDS             1716..3560
                     /locus_tag="VAH76_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEO1387372.1"
                     /translation="MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPATLSYGDIG
                     WEFWKKEGTAIPSRTWQLIASSDTVLLGAITSKPQREAKQELSKALRKSNPYYVSPVI
                     QLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHNLLAESQ
                     HWQTIPADEASCALRLQSKPGLTRLFDFAFNHAMQTGMPRVTLADKPNVLRESGEFAR
                     KIFESTAQKYPKIQADILNVDAVALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGL
                     GLAPSANIGDKGSYFEPVHGSGPRMKKNCANPSAMFLTISMLLNHFGYPDRAKKIVNA
                     VMQVIKEKRYITYDLGGHSTTTDMANAVIERCARLEASCLSTDFNPVPKENLIESDTM
                     GNLLQQLMHCNSAEISDALDSCGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKEAV
                     FHNAANYIDKVPKHSVIVIDNNGQTDCTVWGDLLTHTALRNNIMGTVVHGAVRDVESI
                     RSTNYPVFCTGIYMCSGKNRVYKANEQCPLFINGVTINPGDIVFADDNGVLVIPNELL
                     QEVVNKAIAIRLTEKRIKAAVASGSTLEQAREDYYYEQPWLGINKKRE"
     gene            3563..4423
                     /locus_tag="VAH76_002789"
     CDS             3563..4423
                     /locus_tag="VAH76_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387373.1"
                     /translation="MFSPEKLQFIDIHYHAAPDLYIRRRNAIETGRLYHSLCGAVVLK
                     SHIGSTGIQATIAQEMGLPVFPSVVLNHINGGINYRVVIRALSEYQPLFSGKMIVDFP
                     TITGRKLQSKLSRELTHNNLKQYTRQRETLFGSKQQLRKEVIDILKMARDYPIVLTSG
                     LASREEIYRLIDACNQYDVPSLLLSQPSNPLTGLKCPELNELLENEWLWVEQTALTFI
                     LGHQDKQDFSQILTHLPRVIYSSDLGQTSQMDIPEWLHFSKRIFHELDLSEKRKEELI
                     LSNAIALLNL"
     gene            complement(4685..>5249)
                     /locus_tag="VAH76_002790"
     CDS             complement(4685..>5249)
                     /locus_tag="VAH76_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEO1387374.1"
                     /translation="QLAGMGYIGFAIDMYGNAKLGKDKVEKRALMTPFRENRGKLITR
                     INAAFSKVVQLPEVDSDKVAAIGYCFGGLCVLDLIRSGAPLRGVVSFHGVLLPLEGES
                     NKPLHAKVLILHGYDDPLVPIEQVNQFAIEMTERKVDWQVHVYGQTAHSFTDPNANDD
                     EMGLHYNQLADQRSWQSTQLFLQDLFA"
BASE COUNT         1685 a         1030 c         1004 g         1530 t
ORIGIN      
        1 tcctgtatcg attttagatg cctcattaag agatggtgga catagaacta attttcattt
       61 taaggatgat gatttaaaac aaatactact tcctcttgat aattcgggta ttgaatacat
      121 tgaaattggc taccgtaatg gttcacttca ccctattgaa aatataggga aagcaggaat
      181 gtgcccccaa gattatctcc tgtattgcca atctctaata aaaaaagcaa aaattgcagt
      241 gatggttcat cctcataatg taaacgaaca ggatctgctc gagctaaaaa attgcggtgt
      301 gaaattaata agaatatgta tagcaaaagg cgatcttgta aaagctgtcc ctctgattaa
      361 actggctaag aatttgaatt ttgaaacctc agtgaacatt atccatattt cttattatac
      421 ggaaagcgaa ctggatagtt tgattgagga agtaagcaaa cacgaaccgg atatgatcta
      481 tttcgctgat tctaatggaa gcttatttcc tgacaggata aattgcctgt ataaaaaata
      541 cacccgtcaa tataaagttt cttttggatt tcatgctcat gacaaccttg gattggcgca
      601 agccaacgct cttgcggcga taaacgccgg ggctcaattt attgatgcct cactggcagg
      661 aatgggcaag ggaacaggta atctaaaaac tgaatttttt atcgcttatt tacatgcgaa
      721 caatataaaa aaatataatt tggaaaatgt gctgacagca gctaattatg taagagatgc
      781 gttgaaaata ggacaagaac ccattgaaat gagtgaattc ataaggggaa tttctgatct
      841 gtctacagca gacctgaaaa gctataaacc aggcaagctc tagaaaatac gtacatttcc
      901 aatataggaa tataaggctg ggacaccttg attccccaag atttgatgct ccagaaagca
      961 acgtactttc aaaattaagc tggcaaaatc atagaccttc caggaataag tagagaatat
     1021 aatggcaaaa aaaagattat tgattatcgg ccctgtttca actcaatcct ctagtctgag
     1081 gcacttaagc caaaatttat cttttcttaa tccttattac tccattgagt gtcttgatcc
     1141 tcttgtcacg ctgcaagaat gtcccgatga ggaatattat ggaatttggc aaaatcaact
     1201 aaagccactc atcaaaattt atgatgcatt tgttggcttt tcttttggtg gtgttattct
     1261 tcaacaatgt ttttccatat ttagcgagga aaaaaaacaa atcattcttt tttccacacc
     1321 aacatttgca gattccatct taagagaaaa acttgaaaca gtcatctccc tttgttctca
     1381 aaataagttg gaagaaggat tgtcttcgct ctatcaagca gtttacgcgc ctcattccat
     1441 tcctgatgct tcactgcagt atttaaatag ggaacttgcg atcaagaggc ttatttttgg
     1501 cttaaccaaa gtattgaaaa caaattgtgt ttcgatttta aatcattgta atgtgaatca
     1561 cctgcattta ataggtgaaa aatcacaact ggttaatacc aataacgtga taaaaccatc
     1621 atgtggccaa ttgattgtag ttcccgaagc aagtatgcga gttctggaaa gcaaccccac
     1681 tttttgtaaa gaagctatat taaaggccct gaatcatgaa atccactgat cctataaaaa
     1741 ttgctgtatt accaggtgac gggattggga ttgaagtgac tgaggctacg cttcctgttt
     1801 ttgaagtcct tgatgtccct gcaaccctga gttatggcga tattggctgg gagttttgga
     1861 aaaaagaagg aacagcaatc ccctccagaa catggcaatt gatagcctcc tccgataccg
     1921 ttttgctagg agcgattacc agcaagcccc aacgtgaagc caaacaggaa ttaagcaaag
     1981 cgcttagaaa aagcaaccct tattacgtat ctcctgtcat tcaattaagg caaggtttgg
     2041 atctttttgc aaatgttcgc ccctgttttt ccatcgatga ccaatcaaag ccgtttaatt
     2101 tttgcatcat tagggaaaat agcgaaggat tgtattgcgg atttgattat tttccattac
     2161 caaaagcaat acataattta ttagcagaaa gccaacattg gcaaacgatt ccagctgatg
     2221 aagcaagttg tgccttaaga ttgcaatcaa aacccggttt aacccgttta tttgattttg
     2281 cctttaatca tgctatgcaa actggcatgc ctagagtaac tttggcagat aaaccaaatg
     2341 tgcttagaga aagtggcgaa tttgccagga aaatttttga aagcacagcc caaaaatacc
     2401 cgaaaatcca agcggacata ctcaatgtcg atgccgtagc attatggttg ataaaaagtc
     2461 cggaaaaatt tggagtaatc gttgcggaga atatgtttgg tgatatttta tccgacgtcg
     2521 gagcaggagt tatgggggga ttaggccttg cacccagtgc caatattggt gataaaggga
     2581 gttactttga gccagtacat ggtagcggac caagaatgaa aaaaaattgt gccaatccat
     2641 cggctatgtt tttaacaatt agcatgctgc taaatcattt tggttatcct gatcgagcaa
     2701 aaaaaatagt caatgccgtg atgcaagtca taaaggaaaa gcgttatatt acttatgatc
     2761 tgggtgggca ctccactacg accgatatgg cgaatgcagt gattgaacgt tgtgcgcgat
     2821 tagaggcttc ttgtttatca acagatttta atcccgtccc taaagaaaat ttaattgagt
     2881 cagatactat gggcaacttg cttcagcaat taatgcattg taattctgca gaaatttctg
     2941 atgccttgga ctcttgtgga atcgaaggcg gattattgaa tatcaaaccg ttatcacagg
     3001 gaatgaaaat ggttgggcct gcttatacta tccaatactt acccagagaa aaaaaagaag
     3061 ctgtcttcca taatgccgca aactatattg acaaggttcc caaacattcg gtcatagtca
     3121 ttgacaacaa tggtcaaact gattgcaccg tttggggtga cctattaaca cataccgcat
     3181 tgagaaacaa catcatgggc acggttgtgc atggggccgt gagagatgtc gaatcaattc
     3241 gctcaacaaa ctatcctgtg ttttgcacag gaatttatat gtgctctggt aagaatcgcg
     3301 tttacaaagc caatgagcaa tgccctttat tcattaatgg agttacaata aatcctggcg
     3361 acattgtctt tgctgatgac aatggcgtgt tggtgattcc aaatgaactt cttcaggagg
     3421 tagtgaataa agcaattgcc ataagattaa ctgagaaacg cattaaagca gctgtagcat
     3481 caggttctac tcttgagcaa gctcgagaag attattacta tgaacaaccc tggttaggta
     3541 ttaataaaaa gagagagtaa tagtgttttc gcctgaaaaa ctccaattca tagacataca
     3601 ctaccatgcc gcccctgatc tgtatattcg tcgcagaaat gcaattgaaa cgggaagatt
     3661 gtatcactct ttatgtggtg cagttgtttt aaaaagtcat attggcagta ctggtattca
     3721 ggcaactatt gcccaagaaa tgggtttgcc cgtttttcct tccgttgtgt taaatcatat
     3781 caacggagga ataaattata gagtagttat ccgtgccttg agtgaatatc aacctctttt
     3841 ttcgggtaaa atgatcgttg atttcccaac catcaccggt cgtaaactgc aatcaaaact
     3901 atcaagagaa ttaactcaca ataatttaaa acaatatact cgacaaagag aaacattgtt
     3961 tggatcaaaa caacagctac gcaaagaagt gatagatatt ttaaaaatgg cccgggatta
     4021 ccccattgtt ttaacttcag gtcttgcctc gagagaggag atttataggc taattgatgc
     4081 ctgcaatcaa tatgatgttc cttcattatt gttaagccaa ccgtctaatc ctttaaccgg
     4141 tttgaaatgt ccagaactta atgaattgct tgaaaatgaa tggttatggg tggagcagac
     4201 tgccttaact tttatccttg gacaccagga caaacaagat ttttcacaaa tattaactca
     4261 tctccctcga gtcatttaca gctcggattt agggcagacc agccaaatgg atattccaga
     4321 gtggctgcat ttctcaaaaa gaatatttca tgaattggat ttatctgaaa aacgaaaaga
     4381 ggaactgatt ctctccaatg ccatcgcatt actcaactta taaaattcca atagacctct
     4441 tgcatataac ccgcctcttt cattctattg ctgcagttct catttactgc atgtaaaccg
     4501 cactcttcaa aacgtttgct tcttgcccta aaacaaaata atcagctgat gtaaatcatc
     4561 aggacttacg ataaacccca aggtcgaggc aaaaaaggtt tttaatgagg gagtttagat
     4621 aaactaaatg accgaattaa aaaccttttt taacaaagag attggggttt agcgtaagcc
     4681 ctgatcatgc aaataagtct tgcaaaaaca attgagtact ttgccaggag cgttgatccg
     4741 ctaattgatt gtaatgtagt cccatttcat catcattcgc attagggtca gtaaatgaat
     4801 gagctgtttg gccataaaca tgaacttgcc agtccacttt cctttctgtc atttcaatag
     4861 cgaattgatt aacttgctcg attggtacta aagggtcatc ataaccatgc aatatcaata
     4921 ctttcgcatg taatggctta ttactctcac cctccaaagg caataatacc ccatgaaaac
     4981 tgacaacccc tctaagcggt gccccggaac gtatcaaatc caatacgcac aacccaccga
     5041 aacaatagcc aatagcggcc actttatcac tatccacctc aggcaactga accactttgc
     5101 tgaaggccgc attaatacga gtgattaact ttcctctatt ttctctgaaa ggagtcatta
     5161 aagctctttt ctctacctta tccttaccta gcttggcatt tccatacatg tctatagcaa
     5221 atccaatata ccccatgcca gcaagctga
//
LOCUS       DATNSN010000190         5110 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.190, whole genome shotgun sequence.
ACCESSION   DATNSN010000190 DATNSN010000000
VERSION     DATNSN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.190"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(72..458)
                     /locus_tag="VAH76_002791"
     CDS             complement(72..458)
                     /locus_tag="VAH76_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3757 domain-containing protein"
                     /protein_id="HEO1387375.1"
                     /translation="MKRYYSTLLVLALAHTSLSAYAHTCPDPQTTSLQWGVPPSPWVE
                     NPFSPNSPQGEENTRFVRANILVAGYGQGVTCTYRNSVGDFSILWQVRTKIPARVDYS
                     WIETMGGYVCTRGLNECQFYVADPSA"
     gene            641..727
                     /locus_tag="VAH76_002792"
     tRNA            641..727
                     /locus_tag="VAH76_002792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:675..677,aa:Leu,seq:caa)
     gene            complement(844..2091)
                     /locus_tag="VAH76_002793"
     CDS             complement(844..2091)
                     /locus_tag="VAH76_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387376.1"
                     /translation="MNKRLFIVFILGFSSGLPMALISSTLQAWYANNGMSVLATGALS
                     LVSLPYAYRIFWGPILDRYSLFHLGKRRSWILTMQILLLLGFNLMAWFIPAQYPKLMA
                     FLALVLACFSATQDVAIDAHRAEYLPPQEHALGASLAVFGYRLALLLSGGLALVMAEK
                     LGWAFTYRFMGFAMAIGMLAIFVSKEPSTEIKEKSNFLVSFIAPVKELLSRPGIIYLL
                     IFIFCYKLGEAFTTTTSGIVMPFLIQGLGFSLDTIGYINKMLGIVSILLGGLTAGFLL
                     MRYSLYRALFFFGLLQALTNVFFVILAMSGKNIVLLAIAVFSDNFVAGMGSTALVALF
                     MRLVNKQFTGTQFSMLVALSTLPRIISGPVAATIQMHIGWVGLYQLSIVFALFFIPFL
                     VLIKEQTKERLQSDTAATPIGAA"
     gene            complement(2257..2958)
                     /gene="mip"
                     /locus_tag="VAH76_002794"
     CDS             complement(2257..2958)
                     /gene="mip"
                     /locus_tag="VAH76_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrophage infectivity potentiator Mip"
                     /protein_id="HEO1387377.1"
                     /translation="MKMKLVTAAVMGLAMSTAMAATDATSLATDKDKLSYSIGADLGK
                     NFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD
                     ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINAGNGVKPGKSDTVTVEYTGRLIDGTV
                     FDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGPNE
                     TLIFKIHLISVKKSS"
     gene            3163..4539
                     /locus_tag="VAH76_002795"
     CDS             3163..4539
                     /locus_tag="VAH76_002795"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEO1387378.1"
                     /translation="MNISVFDLFSIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQ
                     RVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGK
                     KEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNANLLIEQVYYSIGGGFITTEEDFD
                     KSTTDTNPPPYPFATATELLKLCKKHHLTIAELMLVNEKTWRSTAEIHKGILDIAKVM
                     DDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSVFEQSDIMNHLNLYAMAVN
                     EENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGA
                     SISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLV
                     QIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGG
                     LAVNLPEC"
     gene            complement(4639..>5110)
                     /locus_tag="VAH76_002796"
     CDS             complement(4639..>5110)
                     /locus_tag="VAH76_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEO1387379.1"
                     /translation="GASWNYAIWGIYHGCLLAVYHVFRFYYIKRNLQTENQLMKIGSI
                     IMTYSLVVIGWVIFRSTSFEQAFTILGKMYDIPGFVQEFAMNQRASAYTDSLFYLVGM
                     FALCFIGPTFKSIETKILSAIQPFWIKKWLYSLSIFTVLIFMFSPVKPFIYFVF"
BASE COUNT         1697 a         1061 c          950 g         1402 t
ORIGIN      
        1 acggtcgcgg ctcgaatttg catcaacagt gcttatcaaa tccgagccgc gaccgtaagg
       61 aagcgggtat attaagccga aggatcagcc acataaaact gacattcatt caaaccacgt
      121 gtgcagacat accctcccat agtttctatc cagctataat caacacgagc gggtattttg
      181 gtccgcacct gccataaaat agaaaaatca cctacggagt ttctataagt acaagtcaca
      241 ccctgaccat aacctgcgac taaaatatta gctcgcacga agcgagtatt ttcttctccc
      301 tgagggctat ttggggaaaa aggattttct acccacggtg atggaggcac accccattgt
      361 aatgacgtgg tttgaggatc aggacatgta tgcgcgtaag cgctcaaact ggtatgagct
      421 aaagccaaaa ctaatagtgt agaataatat cttttcataa agactccatc aattctaatc
      481 ctgataattc caatttattc ttgatatgat ctcattattt aataaagctc aagtaattgt
      541 caaattaata atcaaaatgc aatcaaccat gtaatttttc aaataataaa tgaactaaac
      601 cattgttaga atttatttct ttgctataat gaccgcgcgt gccggggtgg cggaattggt
      661 agacgcgccg gattcaaaat ccggtggtgg tgacaccgtg tgggttcgag tcccaccctc
      721 ggtaccaatc aataaactat caaataattt caatacaaca gataccaaaa actaaaatta
      781 tttaatcctc agttagtcca gagctgtata aaacaaccta ttttgatctg attaaaatag
      841 aaattaagct gcaccaattg gtgtagcagc tgtatcactt tgtaacctct cttttgtttg
      901 ctccttgatc aacactagaa aaggtataaa aaacaaagcg aaaacgatgg ataactgata
      961 taaaccgacc cagccgatat gcatttgaat ggtagcagct acaggacctg aaatgatcct
     1021 gggtaaagtc gataatgcga ctaacataga aaattgtgtt ccagtaaact gtttattcac
     1081 caaacgcata aataaagcaa ccaatgcagt agaacccatc cctgcgacga aattatccga
     1141 aaacactgcg attgctaaca gcacaatatt ttttcctgac atcgctaata tgacaaaaaa
     1201 gacattagtt agtgcttgta gtaagccaaa gaaaaataat gcgcgataca aggaatatcg
     1261 cattaataaa aaacctgccg ttaaaccgcc taacaagata gagactattc caagcatttt
     1321 attgatatag ccgatagtat ccagagagaa acccaagcct tggattaaaa aaggcattac
     1381 tataccgcta gtcgtcgtag taaatgcttc acctaattta taacaaaata taaatattaa
     1441 aagataaata atccctgggc ggcttaaaag ttctttcact ggtgcaataa acgaaacaag
     1501 aaaattactt ttttctttta tttctgtaga gggttccttg ctgacaaata ttgccaacat
     1561 acctatcgcc attgcaaatc ccataaagcg ataagtaaaa gcccagccta atttttctgc
     1621 cattaccaaa gccaaaccgc cagataacaa aagagccaat cgatacccaa acacagccag
     1681 agacgcgccc aatgcatgtt cctgtggggg aagatattct gctcgatgag catcaattgc
     1741 cacatcttgt gtggctgaaa aacaggctaa tactaaagct aaaaatgcca tcaatttagg
     1801 atattgagct ggaataaacc aggccatcag attaaaaccc agaagcagta gtatctgcat
     1861 ggtgagaatc cagcttcgcc ttttccctaa atgaaataaa gagtagcgat caagaattgg
     1921 accccagaaa atacgatacg cataaggtag actgactaaa cttaaagctc ccgtcgcaag
     1981 cactgacatg ccattattag cgtaccatgc ttgcaaggta ctgctgatta atgccatagg
     2041 taaaccggaa gaaaaaccta aaatgaaaac gataaacagg cgcttgttca ttcctttgtt
     2101 cactcagtat gagtcatagc ctttaaaaca gacatatttt aatttcctca gtggtgttag
     2161 gctgacacct tttatgaaga tcattagcct ttacaaagag agcatccctc tctaaatgag
     2221 agggatgcgt tttgtatgac tttaattcaa aaaaacttaa gatgattttt tcactgaaat
     2281 taagtgaatt ttaaatatta aagtttcatt tgggccaata ggtccgccaa cgctacgtgg
     2341 gccatatgca agacctgagg gaacataaat ttcccaagtt gatccagctg gcatcaattg
     2401 caaagcttct gtccatcctg ggataacttg tgaaacctga aaagttgctg gcttaccagt
     2461 tttttcggta ctgtcaaaaa cggtaccatc aatcagacga ccagtgtatt cgacagtgac
     2521 tgtatccgat ttaccaggtt taacaccatt tccagcattg attactttgt attgcaaacc
     2581 acttggcaat acaacaacgc ctggcttgtt tttgttttca gttaaaaagg cttccccttt
     2641 tactttattt tcatccgctt tcttattgaa ttcagcagta cgctttgcca tcaaatcttt
     2701 ctgaaacttg ttaagaacgt ctttcatttg ctgttcggtt aaagccaatt gagcgccact
     2761 catagcgtct tgcatgcctt tagccattgc ttccggatta acatctatgc cttgattttt
     2821 aaaattcttc cccaaatcgg caccaatgct ataagacaac ttatccttgt ctgtagctaa
     2881 tgatgtggca tcggttgcag ccattgctgt tgacattgca agccccataa cagccgcagt
     2941 caccaatttc atcttcataa acaatcccct tttagtctta cacttaagtt ctcatactgc
     3001 acaataaatt gagcaaaatt aaacaaaaac aaatcgccca gtaattctgc ctaaatttac
     3061 ataaaattac cagtcataag ctatcattta attatataat ggacaaaaag gtaatatcct
     3121 tctacacttg cccctaataa tgaacaagag attttaacgg cgatgaacat tagtgttttt
     3181 gatttatttt ctataggaat tgggccatca agctcacaca cagtaggccc tatgttagct
     3241 gccaatgctt ttttgcaatt actggaacaa aaaaatctgt ttgataaaac tcagagagtc
     3301 aaagtagagc tttatggttc gctggcgctt actggcaagg ggcacggtac tgataaagcg
     3361 attttaaatg gtcttgaaaa caaagcgccc gagacagttg atcctgcctc catgatccca
     3421 agaatgcatg aaatccttga ttctaatcta ctcaatttgg ccggcaagaa agaaattcct
     3481 tttcatgaag cgaccgattt tttattttta caaaaagaat tattacccaa acacagtaat
     3541 ggaatgcgct tttctgcttt tgatggaaac gctaatctac tgattgagca agtctattac
     3601 tctattggcg gtggttttat tacaactgaa gaagactttg ataaatcaac aaccgatacc
     3661 aatccccccc cttacccctt tgcaacagca actgagttat tgaaattatg caaaaaacat
     3721 cacttgacca tcgctgaatt gatgttagtc aatgaaaaaa catggagaag tacagcagaa
     3781 attcataaag gaattcttga tattgccaaa gtaatggatg actgcatcaa caacggttgc
     3841 aaacatgatg gagtgctccc cggtggcctt aatttaaagc gacgggcacc tgacttgtat
     3901 agaaaactaa tagaacaaaa aggggttaaa agtgtttttg aacagtcaga tattatgaat
     3961 catttgaacc tttatgctat ggctgtcaat gaagaaaacg cagcgggagg aagaattgtc
     4021 acagccccaa caaatggagc tgccggtatt attccagctg ttcttaaata ttgtcagcaa
     4081 gctcacgaca ggatgagcaa cgaagacatc tacacttatt ttctgactgc cgcagcgatt
     4141 ggaatccttt ataaaaaagg cgcttcaatt tctggcgctg aagtaggatg ccaaggtgag
     4201 gttggtgttg cctcttccat ggcagctgct ggtttgacag cggttcttgg cggaaccatc
     4261 gagcaggttg aaaatgctgc agaaattgca atggaacatc atctgggaat gacctgtgat
     4321 cccgttctgg gccttgtgca aatcccatgc atcgagagaa atgccatggg ggctgtgaaa
     4381 gcagttaatg caacccgaat ggcactcata ggtgatggtc agcatcaaat ctctttggac
     4441 aaagtcatca aaacaatgaa gcaaaccggc atggatatgc aaagtatcta taaggaaact
     4501 tccatgggtg ggttggctgt taacttgcct gagtgttagt tatctcccgc tccctcacgg
     4561 tcgcggctcg gatgattaaa tcaaatgata attaaacctg agccgcgacc gtgagggagc
     4621 ggataacaaa ttatctaatt aaaaaacaaa ataaataaac ggtttcacag gggaaaacat
     4681 aaaaattaac accgtaaaaa ttgaaagcga ataaagccat tttttaatcc agaatggctg
     4741 tatagctgac aagattttcg tttcaattga tttaaaagtt ggccctataa aacacagggc
     4801 gaacattccc acgagataaa acaatgaatc ggtataagct gaagcacgtt gattcattgc
     4861 aaattcctgc acaaaaccag gaatatcata catcttaccg agaatagtaa atgcctgttc
     4921 aaatgaagta gatcgaaata ttacccagcc tatgactact aatgaataag tcattataat
     4981 tgagccaatc ttcatcaatt gattttcagt ttgtaaattt cttttaatat aataaaaccg
     5041 gaaaacatga taaacggcta acaaacaacc atggtatatg ccccaaatcg cataattcca
     5101 ggaagcgcca
//
LOCUS       DATNSN010000191         5099 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.191, whole genome shotgun sequence.
ACCESSION   DATNSN010000191 DATNSN010000000
VERSION     DATNSN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.191"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            49..675
                     /locus_tag="VAH76_002797"
     CDS             49..675
                     /locus_tag="VAH76_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842179.1"
                     /note="macrophage inducible 5; Mig-5; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO1387380.1"
                     /translation="MWTLTKEQQQAITPEKAIELLKEGNKRFVSNLKLNRNLIQQVNE
                     TSQGQFPFAVILSCMDSRTPAELIFDQGLGDIFSIRVAGNILNDDILGSIEFACQVVG
                     VKLIAVVGHTQCGAIKGACDGVKLGNLTNLLNKINPVIQEAKKLDAKHDVHSPEFLNC
                     VTSLNVKHTMNEITQRSDIVHQLLNEKRIAIVGGLYQLETGEVQFFDE"
     gene            complement(793..1086)
                     /locus_tag="VAH76_002798"
     CDS             complement(793..1086)
                     /locus_tag="VAH76_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387381.1"
                     /translation="MNHSVKKKMIISVLYSLRHLIALLVMLVGTYLIKIVTLILYFPS
                     DYSTLSLLSLCRVLWLSNEFFLRFILVVNFIIKPLFLYFGILFWFYYLNKKYH"
     gene            complement(1117..2088)
                     /locus_tag="VAH76_002799"
     CDS             complement(1117..2088)
                     /locus_tag="VAH76_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEO1387382.1"
                     /translation="MSLRILSLSDVKHSITMHQAISALESAFIQLANNTVKLPLRTGV
                     TIEEENGLMLTMPGYLAQEKSLGLKVVSIFPENLAKNKPTITGFIMLLDATTGEAKVL
                     MDAAYLTSLRTGAVSGLATQYFARDNATRVAIIGSGAQAKTQLEAVASVRDMQQVFVW
                     SRNIKNAEKFAKEFEDQFDMTICSSVAMAVKDADIICTATNSTEPLIHFGDISPHVHI
                     NAIGSHHPSMKEISHDVLSRAVVIADQLDAVLAESGEIISAVEHNHLKKESIIEIGQW
                     LLQKNTDCKDKLTVFKSVGLAIQDLSVANAVYQNALKKNLGELFSIS"
     gene            complement(2143..2589)
                     /locus_tag="VAH76_002800"
     CDS             complement(2143..2589)
                     /locus_tag="VAH76_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEO1387383.1"
                     /translation="MKSIANKENEVAIIHKLIEEYANAARDKDIDKIMSHYAPDIRSF
                     DAVSQLQFKGADDYRKHWQTCLSFCPGPSVFEVHQLETLVDNNLAVSYYLTYCGGTNE
                     KGETQGGWMRGTMVHCKMNGKWKIMHEHYSIPFDMKTGNTLFDLKP"
     gene            complement(2781..3674)
                     /locus_tag="VAH76_002801"
     CDS             complement(2781..3674)
                     /locus_tag="VAH76_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEO1387384.1"
                     /translation="MSVEEFIIAVYCLVDDIMNELLNSKSLRQRGFNPALTDSEMIAM
                     ELVAEFQGIDTDKGAWEYFCNHWRALFPNIGSRANFAKHAANLWNMKQQIQKVLAGRM
                     GAFTDLLHISDGFPVPVCHFRRANFSQVFAGEATYGYCASKGETYYGFKGNVLINSDG
                     IITGITLSQAHIDERESLWDLVDGIHGMIIADKGLIGSEYQNELREYSGIDLQTAVRS
                     NMEDKRGKHFSKWLTSTRRLVETVIGQLTERFQIEKVRARKLWYLTNRVARKVLAHTV
                     CAYINKKMGNPPLQFELLVKS"
     gene            3815..4714
                     /locus_tag="VAH76_002802"
     CDS             3815..4714
                     /locus_tag="VAH76_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HEO1387385.1"
                     /translation="MFQAGQKLFERIRRKKRMASANHDVHLLARTINEWLPQGIQSLI
                     EGTYTPRFLKRYYFKDEMLDQLYLADRVLQNLLLQQLKPTFPYVMNPNCYHVHGPSGV
                     QLAAQRIRETLATKHYKYIIRADIKSYYKSIQHHVLIEDIKRYYFDTKVQLMLEQIVR
                     NPIETPRGYKNPDNGIALRGPLSQFFSALYLKPLDDAFDTLDVTYLRYQDDIIILCHS
                     KRQLERCKRRLMTILKERHLQLSRKKTRIGAIEGGFHFLGINYLEPQSPGSTNVTQDI
                     REMAVNAPLTDDSCLLFNQTGGG"
     gene            4812..5048
                     /locus_tag="VAH76_002803"
     CDS             4812..5048
                     /locus_tag="VAH76_002803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387386.1"
                     /translation="MVIDGFSIPRIRSYLTAWSRWWVRTSQVWSHQTLLIQFIRSCYD
                     PQIAGIGAEILLINLRRMDQPAGMMAQPDWQESV"
BASE COUNT         1591 a         1063 c          926 g         1519 t
ORIGIN      
        1 aattcaaaaa ctaaaaatat tcaagttaaa ttaataggaa tttaaattat gtggacttta
       61 accaaagaac aacaacaagc aattactcca gagaaagcta ttgagctctt aaaagaaggc
      121 aataagcgtt tcgttagtaa ccttaagtta aatcgcaact tgatccagca agtcaatgaa
      181 acatctcaag ggcaatttcc atttgctgtt attttaagtt gtatggattc acgcactcca
      241 gcagagctga tttttgacca aggccttggc gatattttta gtattcgcgt tgcaggtaat
      301 attttaaatg atgatatcct aggcagcatt gaatttgcat gtcaggtagt aggtgtcaag
      361 ctgattgcag ttgtcggcca tactcagtgt ggggcaatta aaggagcctg tgatggtgta
      421 aaattaggca acctgacgaa tctactgaat aaaatcaacc cagtgattca ggaagccaaa
      481 aagctggatg caaagcacga tgttcatagc ccagagtttt tgaattgcgt tacgagcctg
      541 aatgtaaagc ataccatgaa tgaaattacc caacgcagtg atatcgtaca tcaattgctt
      601 aatgaaaaaa gaattgcgat tgtaggtggt ttataccagc ttgagacagg agaggttcaa
      661 ttttttgatg agtgatatca tgatattaat aactttcaat aattagtctt ttcaactgtg
      721 caacatttct gatacctaaa gcttttacaa ggaattgatg cgggcttggt gcttatagtc
      781 cgcattcatg aatcaatgat attttttatt caggtaataa aaccaaaata aaattccgaa
      841 atagagaaaa aggggtttaa taataaaatt aacaactaat ataaagcgta aaaaaaattc
      901 attggacaac cacaagacgc ggcacaaaga caaaagtgaa agcgttgaat aatcagaagg
      961 aaaatacaat attaatgtta ctatcttaat caaataagta ccaactaaca taaccagtaa
     1021 agcaatcaga tgacgtaagg aatataaaac tgaaataatc attttctttt ttacagaatg
     1081 attcatttat actcactcta ctcggtttta cccttactaa cttatagaaa acaactcacc
     1141 cagatttttc ttcaatgcat tttgataaac agcattggca acacttaaat cctgtattgc
     1201 caagccaacg gatttaaaca ccgtgagctt atctttgcag tcagtattct tttgtaataa
     1261 ccattggccg atttcaataa tggattcctt ttttaaatga ttgtgctcta ctgcactaat
     1321 gatttcgcct gattcagcta aaacagcgtc taactgatca gcaatcacca ctgctcggct
     1381 taacacatca tggctaattt ctttcattga ggggtgatga gaacctatgg cattaatatg
     1441 gacatgtggc gaaatgtctc caaaatggat cagtggttct gtgctattgg ttgctgtaca
     1501 aataatatcg gcatctttca ctgccatggc gacagaagaa caaattgtca tatcaaattg
     1561 atcttcgaat tctttagcaa atttttcggc atttttaata ttgcgcgacc aaacaaaaac
     1621 ctgttgcata tctcgcacag aagcaaccgc ctcaagttgt gttttcgctt gagcacctga
     1681 cccaatgatc gcgacccgag tcgcattatc gcgtgcaaaa tattgtgttg ccaaccctga
     1741 aacagcgccc gttcttaaag aagtgaggta agcagcatcc attaatactt tagcttcacc
     1801 tgtcgttgcg tcaagcaaca taataaaacc agtgatagta ggtttatttt tagccaaatt
     1861 ttcaggaaaa atggatacta cttttaagcc taagcttttt tcctgggcaa gataacccgg
     1921 catagtcaac attaatccgt tttcttcttc aatagtaaca cccgttctca agggcaactt
     1981 caccgtgtta tttgctaatt gaataaatgc gctttccaaa gcactgattg cctgatgcat
     2041 ggtgatgcta tgctttacat cgcttaagga caatatcctg agactcatat caataaaccc
     2101 tatactttag ttaactacgt agtgactgca tagaatcaca tcttatggtt ttaaatcaaa
     2161 taaggtattg cctgttttca tatcaaaggg aatagaataa tgctcatgca tgattttcca
     2221 ttttccattc attttgcagt gtaccattgt acctcgcatc caccctcctt gcgtttcacc
     2281 tttttcgttc gttccaccac aataagtcaa ataataggaa accgccagat tattatcaac
     2341 caaagtttcc aactgatgca cttcaaatac tgaaggtccc ggacagaatg aaagacaggt
     2401 ttgccaatgt tttctataat catcagctcc cttaaactga agctgtgaaa ctgcatcaaa
     2461 agaacgaata tctggtgcat agtgggacat gattttatcg atgtctttat cgcgtgctgc
     2521 attggcatac tcttcaatga gcttatgaat tattgccacc tcattttcct tattcgcaat
     2581 agatttcacc tttatctcct ttaattaact tcttcctgaa ttttaattgt agcaaattct
     2641 gcaaaaaacc taatgaattg ttcacctgta acagagtctg ttgcttttgc aactcaagcc
     2701 agtaaaaagg tcaagagatc ctgttacaac ctaaattatc tactatggtt aattatataa
     2761 taaacgccat tctcaacttt ttatgattta accaacaact caaattgaag aggcgggtta
     2821 cccattttct tgttaatgta ggcacaaacc gtatgtgcca agacttttct tgcaactcga
     2881 ttggttaaat accaaagctt gcgggctctt actttttcaa tttggaagcg ctctgttaat
     2941 tggccaatga ctgtttcaac aagacgtctg gtagatgtta accacttact aaagtgcttt
     3001 cctcgtttat cttccatatt agagcgtaca gcagtttgta aatcaattcc tgagtactct
     3061 cgtaattcgt tttgatactc agaaccaatt aatcctttgt ctgcaataat catcccatgt
     3121 attccatcaa ctaaatccca taaagactcc cgttcatcaa tatgagcttg tgatagagta
     3181 atccctgtaa taatgccatc tgagttgatc aatacattgc ctttaaagcc ataataggtt
     3241 tcccccttgg atgcacaata accataagta gcctcacctg caaatacttg actaaaattc
     3301 gctcttctga aatgacaaac gggtacagga aaaccatctg aaatatgcaa taaatcggta
     3361 aatgccccca ttcgtccggc taatactttt tgaatttgtt gtttcatgtt ccataaattt
     3421 gctgcatgtt tagcaaaatt tgctcgtgaa ccaatatttg gaaatagcgc acgccaatgg
     3481 ttacagaaat attcccaagc ccctttatcg gtatcaatcc cttgaaactc agcaaccagc
     3541 tccatagcaa tcatttcgct atctgttagc gcaggattaa atcctctttg gcgtaaactc
     3601 ttgctgttta ataattcgtt catgatatca tctaccaaac agtacacagc aatgataaac
     3661 tcttctactg acatagcctt ttccttgtag tttcttttct gcaaacagta acttattgga
     3721 attggctttg ttgttcaaca gaccacttta tttcagtact gtggcacaag aaataaccag
     3781 gggcctcatg aaaaaacgct gggattttac accgatgttc caagcaggcc aaaagctttt
     3841 tgaacgtatt cgtagaaaaa agcgaatggc ctcggcgaat catgacgtgc atcttcttgc
     3901 tcgcaccatc aatgaatggc taccccaagg cattcaaagc ctgattgaag ggacctatac
     3961 gccaaggttt ctcaagcgat attacttcaa ggacgaaatg cttgaccagc tctatttggc
     4021 agatagagtc ttgcaaaatc tgctactcca gcaattaaaa ccaacatttc cctacgtcat
     4081 gaacccaaat tgttaccacg tgcatggccc aagtggggtg caattggcag cgcagaggat
     4141 tcgagaaaca ctggcaacaa aacactacaa atacattatc agggcagaca ttaaatcgta
     4201 ctacaaatcc atacagcatc atgtattgat tgaagacatc aagcgctatt atttcgatac
     4261 caaagttcag ttaatgctgg aacagattgt cagaaaccct attgaaacgc caagagggta
     4321 caaaaatcct gataatggca tagcgcttcg cggccctttg tcccaatttt ttagtgcact
     4381 gtatttaaaa ccactggacg atgcatttga taccctggat gtgacctatt tacggtatca
     4441 ggacgacatc attattttgt gccacagcaa acgccaatta gaacgctgca aaagacggct
     4501 gatgacgatt ttaaaagaaa ggcatcttca gttatctcga aagaagacac gtattggcgc
     4561 tatcgaaggt ggttttcatt ttttaggcat caactatctg gaaccgcaat ctccaggtag
     4621 cactaatgtg acgcaggata ttcgcgaaat ggccgtcaat gccccattga cggatgattc
     4681 ttgtttgtta tttaaccaaa ctgggggggg gtaataacaa ctcgataaag ccaaatcaag
     4741 aatatcctga gccaatcacc attgttcctc acccaaggac aatcagaaag gcgcgtgagc
     4801 aagttcggct tatggttatt gatggatttt ctatcccccg aatcagaagc tacttgactg
     4861 cgtggtcacg ttggtgggtg agaacaagtc aagtctggag tcatcaaacc ttgttaatcc
     4921 aattcatccg cagctgttat gacccgcaaa tagcgggtat tggcgccgaa attctcttga
     4981 ttaacttgcg gagaatggat caacctgcag ggatgatggc gcaaccagat tggcaagagt
     5041 ctgtttaagg tcgtctgttc tcgctttatc aagggcttct atagccgcta aatccgccc
//
LOCUS       DATNSN010000192         5005 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.193, whole genome shotgun sequence.
ACCESSION   DATNSN010000192 DATNSN010000000
VERSION     DATNSN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.193"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            230..1471
                     /gene="mavL"
                     /locus_tag="VAH76_002804"
     CDS             230..1471
                     /gene="mavL"
                     /locus_tag="VAH76_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavL"
                     /protein_id="HEO1387387.1"
                     /translation="MAYQLLLSKETLNKILQYKQNIEKGLATPGKFFLEELSKQEKSI
                     SEMDITTFTQLLIQSKKPQVFAESQVYHDGTDWTLEEESILGDVSVNMPVTMYNDGGH
                     GSSFKNHPEPISGYLAYVPGALLASGSGPTSDMKEVLDNGKLNKDKLNALYERRLLPQ
                     LIHFNELARQNEKQAAITIPGIGTGCFSGAYYDVIKPYVRNALIHILEKHKDSLPYID
                     IIHYDPYMGDEPAEKKIGHMSFRVSPSGVVRGTTGQLDYPLGSNPDTHILVSIVAWDH
                     FSWPGNDYWGGARQTDDGVKAASTDTMGQVTGVSGVYDKKWGRYMPPESFTKDRKGMS
                     DWGDYVRENGIVFNGPVLALDKSGKLDTLENVASRSKAKVETNAIDVLVRGMLSLFSH
                     HTTTEPSPTVKEEESKKSGPQ"
     gene            1898..3028
                     /locus_tag="VAH76_002805"
     CDS             1898..3028
                     /locus_tag="VAH76_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEO1387388.1"
                     /translation="MKEKYIGLHKFELDTPCLVIDYEKLKHNLEIMQQHGFTWGIGIR
                     PHCKTHKCTQIARLQLEYGAIGISAAKLSEAAALVSKRITNVLITSLIVSEAKYSRLF
                     NCLKSAPDLMLVVDDKDNIRMLNKLGKSLNQPVHVLLDIDSGIGRTGINPEAALEYGH
                     FINGMNWLKLKGIQCYAGHLQHIQNYEERKAFSLQVMEMASRIVKSFRDANLPCEILT
                     GTGTGTFDIDVEASEVTEIQPGSYAVMDVEYGSIGSKNNPSSFQLFKNSMTLLTSVIS
                     SNRREHATVDAGTKSIYVDPHHKPHIISHPGLVYDWGGFGDEHGKVFSCEGGSHFLQN
                     GDVLEIIVPHCDPTINLYDKFYITENNVVVDIWDIDMRGKSQ"
     gene            complement(3069..3248)
                     /locus_tag="VAH76_002806"
     CDS             complement(3069..3248)
                     /locus_tag="VAH76_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEO1387389.1"
                     /translation="MLTWALIFFIIAIIAAAFGFGGIAVAAAGIAKILFFLFLVMFVI
                     FLIMGLLGRRGPPPV"
     gene            3470..3838
                     /locus_tag="VAH76_002807"
     CDS             3470..3838
                     /locus_tag="VAH76_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387390.1"
                     /translation="MQRTKAHYSKIFFVLFLFFCTGSSALFACPCFNKGFLHVAFIDD
                     KDTECRIYKEGSIIYKIEIFDKEKIASSTHTQCNLNTEHHDVFMNYDFHYLDDHGDCI
                     REILDACNKLKINSLHMDDE"
     gene            complement(3930..4460)
                     /locus_tag="VAH76_002808"
     CDS             complement(3930..4460)
                     /locus_tag="VAH76_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387391.1"
                     /translation="MSKFPHKNPETLRQYFREKNLEQLLEINQSYGPHFEKLENAIGR
                     QTEEISTRTEKLSWLLKDKKELSQNSEKIAIEQEQYERNRASIMSDSCTGAERYLALT
                     ALGMSPLDSYHQHSSNIQNEIDTISKRLNELNSHLALWKNQKSKAISELKILNSVIEE
                     KRKEFQVDHQFALSSN"
     gene            complement(4680..>5005)
                     /locus_tag="VAH76_002809"
     CDS             complement(4680..>5005)
                     /locus_tag="VAH76_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387392.1"
                     /translation="LGQKYGSFFWGCTKFAISPYTNTFLSIVKSKEVEEADCLSKALA
                     ASIVCGILTFVVPVLPVLCAFTFTLSSIAMLLAVASMFVTYPIAILGDALCGPELSDD
                     YHYSF"
BASE COUNT         1596 a          866 c         1004 g         1539 t
ORIGIN      
        1 ccagttttga aaacggcata tcaggttttc actgattgga atagcagtca ttatgtgtta
       61 tcattatagt catattgact aattaagttc ctttggttga ggcaataggt ttgagattgt
      121 ttcagtacaa tcaacacatc tgtaggggaa gatttccagt aagcatgctt attgtaagga
      181 cttcgtggta agaaatatta atacaatttt tggattaagt gagaatataa tggcctatca
      241 attgttgctc agtaaagaaa ccctgaataa aattttacaa tacaaacaga acatcgaaaa
      301 agggcttgcc acgcccggta aattttttct tgaagaatta agtaaacaag aaaaaagcat
      361 ctctgaaatg gatattacta catttactca actattaatc cagtctaaaa aaccacaggt
      421 ttttgctgaa agtcaggttt atcatgatgg aactgactgg acactggaag aagagagcat
      481 tcttggagat gttagtgtca atatgccagt cactatgtac aatgatggtg gacatggctc
      541 ctctttcaag aatcacccag agccaatttc cggttatttg gcttatgtgc caggcgccct
      601 cttggcttcg ggcagtggac cgacttcaga tatgaaagaa gtgctagaca atggcaaact
      661 aaataaagac aaattaaacg ctttgtacga gagacgtttg ttacctcaat tgatccattt
      721 taatgagtta gcgcgacaaa atgaaaagca ggcagcaatt actattcccg gtataggaac
      781 agggtgtttt tcaggcgctt attatgatgt gattaaaccc tatgtcagaa acgcattaat
      841 ccatattctt gagaaacata aagattcgtt accttacatt gatatcattc attatgaccc
      901 ttatatggga gatgaacctg ctgaaaagaa gattggtcat atgtcttttc gtgtgagtcc
      961 ttctggtgtg gttcgtggta caacggggca acttgactac cccttaggta gcaatccaga
     1021 tacgcatatc ctcgtttcaa ttgttgcctg ggatcatttt tcctggcccg gaaatgatta
     1081 ttggggcggc gctcgtcaaa cagatgatgg tgtcaaggcc gctagcacgg ataccatggg
     1141 gcaagttaca ggcgtatctg gtgtctatga taaaaaatgg ggtcgatata tgcctccaga
     1201 atcatttact aaagaccgta aaggtatgag tgactggggg gattatgtca gagaaaatgg
     1261 cattgtattt aatggccctg ttttagctct ggataaaagc ggtaaattgg ataccttgga
     1321 aaatgttgca agccgttcca aagcaaaagt ggagactaat gcaatagatg ttcttgtgcg
     1381 cggcatgcta agcctttttt cccatcatac tacgacagag ccctctccaa ctgttaaaga
     1441 ggaagaatcg aaaaaatcgg gtcctcagta ataatgtagt ttgacaggca aaaacaaggt
     1501 ctgacttttt cggtcaggcc ttcacattat gctctggtaa agcaaagcga gacaacccaa
     1561 ttaatctatt tttttaactg agatatctgc ttttcaacga gatacgagag catgcgttta
     1621 atcatttttt cgagactttt tatttcttgc tgtttcttct tgaatttagt tttactgaac
     1681 cactttaagt ataatgacac agtcatagac agtgcaggag ttatattttc aagttcaaac
     1741 ataggttagt aaattactac cttttcaggc tttattaaca cttgttgggg gtaaatgcct
     1801 aacgcctcaa gtgatctaaa taaagattta aattatggca agcataagta atttatgtca
     1861 taaaacgcat ttatttaaca ttgtgatgaa ttatctcatg aaagaaaaat acattggact
     1921 tcataagttt gagctagata ccccatgtct tgtgattgat tatgaaaaac tgaaacataa
     1981 tttagaaata atgcaacaac atggcttcac atggggcata ggaataaggc cacattgtaa
     2041 aactcacaaa tgtactcaaa tcgcacgttt acaacttgaa tatggggcaa tagggatttc
     2101 tgccgccaaa ctatcagagg ccgcagcatt ggtatccaaa cgaatcacga atgtcttaat
     2161 cacttcactc attgtatcgg aggccaagta ttcaagatta ttcaattgtc ttaaatcagc
     2221 gcctgattta atgctggttg tagatgacaa ggataatatt cgcatgctta ataagctcgg
     2281 aaagtcgcta aaccagccag ttcatgtctt gcttgatatc gattcaggca ttggccgaac
     2341 aggcataaat cccgaggcag cattggaata tggtcatttt atcaatggga tgaattggct
     2401 taagctaaaa ggaattcaat gttatgcagg ccacttacaa cacattcaaa actatgaaga
     2461 gagaaaagca ttctctctac aggttatgga aatggccagc aggattgtta agagttttag
     2521 agatgccaac cttccttgtg aaatattaac tggcaccgga actggtactt ttgatattga
     2581 tgtcgaagca agtgaagtaa cagaaataca gcctggctca tatgctgtaa tggatgtaga
     2641 gtatggttca attggttcaa aaaataaccc atcaagcttt cagctattta aaaactcgat
     2701 gactctttta acgagcgtga taagcagtaa tcgtagagag catgccactg ttgatgcagg
     2761 aacgaaatcg atttatgtcg atcctcatca taaaccacat attattagtc atcccggact
     2821 ggtttatgat tggggtgggt ttggagatga gcatggcaag gtattttctt gcgaaggcgg
     2881 aagtcatttc ctgcaaaatg gcgatgtttt agagattatc gttcctcatt gtgatccgac
     2941 aataaacttg tatgataagt tttatatcac agaaaataat gttgttgtcg atatttggga
     3001 tatcgacatg cgtggaaaat cacaataatc tatactatac aaccaggtgt ttgagcttca
     3061 tatttctgct aaacaggagg agggccacga cgtccgagta atcccataat aagaaatatg
     3121 acaaacatca ccaaaaatag aaagaataat atttttgcaa ttccagcggc agccactgca
     3181 ataccaccaa atccaaacgc ggctgcaata attgcaatga taaaaaaaat aagtgcccaa
     3241 gttaacataa tacctcctgt aggacttacg aaaaacccca aggtcaaggc aaaaaaatgt
     3301 ttttaatgag ggagtttaga ataaactaaa tgaccgaatt aaaaacattt ttttaacgac
     3361 gagattgggg ctttacgtaa gtcctgtccc tgtcaagatt atccttttta ttaattatag
     3421 accaataatg gtctacaatt agacaaaaga cagtcaatca gggaagaata tgcaaaggac
     3481 taaagctcac tatagcaaga tattctttgt cttatttctt tttttctgca ctggttcaag
     3541 cgcattattt gcctgccctt gctttaacaa gggcttttta catgtagcct ttatagatga
     3601 taaagatacg gaatgtcgta tatataaaga aggtagtatt atctataaga tagagatttt
     3661 tgataaggaa aaaattgcgt caagtaccca tactcaatgt aatctgaata cggaacatca
     3721 tgatgtattt atgaattatg acttccatta tctcgatgat catggagatt gcatcagaga
     3781 aattctggat gcgtgtaata aactgaaaat aaattccttg catatggatg atgagtaaga
     3841 caggacaagg tgaaataggg taggaattga ataattaggg ctatcacccg gattatagcc
     3901 aagataatcc gggtgagttt tatcaatgtt tagtttgaac tcaatgcaaa ctgatgatcg
     3961 acttggaact cttttctttt ctcttcgata acactgttta gtatctttag ttcagaaatt
     4021 gcttttgatt tttgattttt ccataaagca agatgagagt ttaactcatt taatctttta
     4081 cttatcgtgt cgatttcatt ttgtatattc gagctatgtt gatggtagct atccaagggt
     4141 gacattccaa gagcggtgag tgctaaatag cgttctgctc cagtacatga gtcactcata
     4201 atcgatgctc gatttctttc atactgttct tgttcaatag cgattttttc agaattctgc
     4261 gaaagctctt tcttatcttt taaaagccag gataactttt ctgtgcgagt cgatatctct
     4321 tcagtctgtc taccaatcgc gttttccagt ttttcaaaat gaggcccgta agactgatta
     4381 atttcgagta attgttccag atttttttct cggaaatatt gacgaagtgt ctctgggttt
     4441 ttatgcggga atttagacat gtttctacct ttttgaatca aaatcaacgt atcttaacat
     4501 atgcttttta atcatatcaa acatttatgc ttcataatgg gtgaattgag ggctatttat
     4561 tgatgattgg tttacttttt ctcaacatca agcaagagga gtatttttac gagtttaaaa
     4621 atactcttcc tgattgttga tacttatgat gatttcaaat gaaattatct taaaactgat
     4681 tagaaagaat aatggtaatc gtcagacaat tctggtccgc acagtgcatc accaagtatt
     4741 gcaatcggat aagtaacgaa catactggct actgcaagta acatggctat tgaagacagg
     4801 gtaaatgtaa aagcacataa tactggtaat accgggacaa caaaggtaag aattccgcag
     4861 acaatgctgg ctgcaagagc cttggataag caatctgcct cctcaacctc ttttgatttc
     4921 acgatggata gaaaagtatt ggtatagggg gaaatagcaa atttagtaca tccccaaaag
     4981 aatgaaccgt atttttgacc taatg
//
LOCUS       DATNSN010000193         4989 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.194, whole genome shotgun sequence.
ACCESSION   DATNSN010000193 DATNSN010000000
VERSION     DATNSN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.194"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..239
                     /locus_tag="VAH76_002810"
     CDS             <1..239
                     /locus_tag="VAH76_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387393.1"
                     /translation="IPTFLDMLAELKQSTFKALASLGKTLSAWKDEVARMWRFSKSNG
                     ITEGFHRKMKLIQRRAYGFRNFENYRVRVRVLCG"
     gene            519..1748
                     /locus_tag="VAH76_002811"
     CDS             519..1748
                     /locus_tag="VAH76_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEO1387394.1"
                     /translation="MAEAEKGIKLTKTIVDKLEHIPGKTQTFFRDNDLKGFALRITSG
                     GVKSFIVETRINGKVKRVTIGKYGNLTVEEARKQAKSLLGSVARGDDPIAEKKTKKVH
                     AMTLQQVLNDYLKARKDLKPRTLNDYQCVLHEVVPDWLDKPLINITREMIAKRHTKHG
                     QANSKARANNAMRVLRAIFNYAMYEYQDGNGHPIITINPVKYLSHTRAWYRVDRKQTV
                     IKPHQLADWYKAVIVLVETDNYRNALLWHDYFLLLLFTGMRKMEAASLRWEDIDLKSK
                     TITLQDTKNHEIHTLPMSDFVYELMERRSRNKNSEFVFPAESKTGYIYEPKKAVNSVV
                     ELSGVPFTLHDLRRTFATIADSLDLPAYALKRLLNHKMNNDVTAGYIMKDLERLRKPM
                     QQVANFILTHMMETAEL"
     gene            1856..2461
                     /locus_tag="VAH76_002812"
     CDS             1856..2461
                     /locus_tag="VAH76_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387395.1"
                     /translation="MTAIANIREYISKLPEGQTFASSELRQFASTDNIRQILNRLVKS
                     NEIKKVARGIYVKPKTLSIGEKLPLASEIAEAIAKATGETIMVHGAEAARQLQLTTQV
                     PTRQVFYTDGNSRTLTIANRTVRLKHVNPSRMIEPGTIAGLVITALIYLGQENVTTST
                     LHLIKKRIPEADFKKTINLISRMPAWLADIFFRYQKEGCNA"
     gene            2523..3467
                     /locus_tag="VAH76_002813"
     CDS             2523..3467
                     /locus_tag="VAH76_002813"
                     /inference="COORDINATES: protein motif:HMM:NF020419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEO1387396.1"
                     /translation="MDVSEVVIEKDLWVCWLLEQLFTLPVRMVFKGGTSLSKVFNIIK
                     RFSEDCDITIDYRDFAEKEIDLEKTSRSQLKKISEQLKGQLKHYVHDTVLPLLTEKLK
                     NQFPEQKFEIILSEDGETLEFYYPSIMTDSIVYLRDHVLIEFGIRNSTEPCEKHQILP
                     YLTSSIGSDIGVPMPLINTLSPIRTFWEKVTLIHVECHRERLHQTPERLSRHWYDLYM
                     LNHSQIGDEALERLDILESVLEYKRAFFNASYANYDDCQKGKLLLIPSSENKEALSKD
                     YAHMIDADMFYQTPPSFAEIIVALESLEFKINQKVLAV"
     gene            3497..4474
                     /locus_tag="VAH76_002814"
     CDS             3497..4474
                     /locus_tag="VAH76_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO1387397.1"
                     /translation="MRRILLISDTHGNLDIINEKVAETMADMVIHAGDFGFYDEQSIY
                     RLSQRELRLLVSHSPVWRQYEVDKQTNRERLIEIVKEHQLLGDFPDYISDKKRFLVPV
                     YAVWGNHEDVQVLRQLNANLNVENLHVLDEHHFYQFHTSENELEFSLYGLGGNFLVGK
                     KLFDKPIAGNGGKVWTALHQFGVLYQRIKNKSKPSIFVSHVSPGKEPLLSRLIMHFMP
                     NFWISGHMGAPFTCTWNQFTIREMNESLDWLESDIDLIEEQYQQGRLTDEAALAYEII
                     KRPIDRDESWYKKLWNINLPDVVDGHAVLILEGNRFSLETYSKGIKFNN"
BASE COUNT         1666 a          830 c         1023 g         1470 t
ORIGIN      
        1 taatccctac attcttggat atgctagctg aattaaaaca gagcaccttt aaagcgttag
       61 catcattagg aaaaacgctt agtgcttgga aagatgaagt cgccagaatg tggcggttta
      121 gtaaatctaa tggaataact gaaggctttc atcgcaaaat gaaactcatt cagcgaaggg
      181 cttatggttt tagaaatttt gaaaattata gagtgcgtgt tagggtgctc tgcgggtgat
      241 taaagactgc ccccttaaat gggaaagagc ctaaaattcg ccctctataa ctacacgtaa
      301 ctacttgatt ttattggtgg gcccactagg acttgaacct aggaccaacg gattatgagt
      361 ctgtaaaata gtggttttag cacgttttat caaattttaa attctttcaa aaacctttta
      421 aaatcaatgt ttataagcca taatttgcca tattggaatt tatttagttt taaataaagt
      481 gtctacatgg cgtctacatg gaattttcga ggtgtattat ggcggaagct gaaaagggta
      541 ttaaattaac caaaaccatc gttgataaac ttgagcatat tccgggaaaa acccaaacgt
      601 tttttcgtga taatgatttg aaaggctttg ctttgagaat tacttcaggt ggcgttaaaa
      661 gtttcatcgt tgaaaccaga atcaacggca aggttaaaag agtcactata ggtaaatacg
      721 gcaacttgac ggttgaagaa gccagaaagc aagccaaaag ccttttaggt agcgtggcca
      781 gaggtgatga tccgatagcg gagaagaaaa caaaaaaagt tcatgccatg accttacagc
      841 aagtgttgaa tgactacctt aaagcaagga aggatttaaa accacgtacc ttaaacgatt
      901 atcaatgtgt gttgcatgaa gtagtgccag actggctcga taaacctctg atcaatatca
      961 cccgagaaat gattgccaaa cgacacacta agcatggtca ggcaaatagt aaagcacgtg
     1021 ctaataatgc catgagagta ttaagagcta ttttcaatta tgctatgtat gaatatcaag
     1081 acggcaacgg acatcccatc atcaccatta acccggtcaa atacttatcg catactcggg
     1141 catggtatcg agtggatcgc aaacaaactg tgatcaaacc gcatcaatta gctgactggt
     1201 ataaagcagt tattgttttg gtggagactg ataactatcg aaatgccttg ctgtggcatg
     1261 attatttttt attactctta ttcaccggca tgcgaaaaat ggaagcagcc tctttgcgat
     1321 gggaggatat tgatctcaaa tccaaaacga tcactctgca agacaccaaa aaccacgaaa
     1381 tccacacatt acccatgtct gattttgtat atgagctaat ggaacgcaga agtcgtaata
     1441 aaaacagtga atttgtattc cctgctgaaa gtaagacggg ctatatctat gaacctaaaa
     1501 aggcagtgaa tagtgtagtg gagttgtctg gcgtaccctt tactttgcat gatcttcgta
     1561 ggacctttgc gactatcgca gatagtcttg atttaccagc gtatgctttg aaaagattac
     1621 tcaaccataa gatgaacaat gacgttacag cagggtatat catgaaagat ttagagcggc
     1681 taagaaaacc catgcagcaa gtagccaatt ttatactgac acatatgatg gagacggctg
     1741 agttatgaat attaataaat caatagaaca tttggggaag tagattattc tcaaacttaa
     1801 tttcaatttt gtcacgtttt atactataat tcgtgacaaa tgaggaaatt aaattatgac
     1861 agcgattgct aacattcgag aatatatttc taagctgcct gaaggacaga cctttgcttc
     1921 tagtgagttg cgacagtttg cttcaacaga taatattcga caaattttaa atcgcttggt
     1981 taagtcaaac gaaattaaaa aagttgcccg tggcatttat gttaaaccaa aaacgttaag
     2041 tattggggaa aagttgccgt tggcatcaga aattgctgaa gctatcgcca aggctaccgg
     2101 tgaaacaatc atggtgcatg gcgcagaagc agcaaggcaa ttgcagttaa caacccaagt
     2161 tcctacacgt caggtatttt atacggatgg aaattcaaga acgttgacca tagccaatcg
     2221 aactgtcagg ttgaagcacg ttaatccaag cagaatgatt gaacctggga caatagcagg
     2281 attagtgatt acagcactta tctatttagg gcaagagaat gtgacaacgt caacgctcca
     2341 tttaattaaa aaacgtatcc cagaagcaga ttttaaaaaa accataaatt taataagccg
     2401 gatgccggca tggttagcag atattttttt cagatatcaa aaagagggct gtaatgcata
     2461 attccttttt caatttaaca ttagaagaaa gacaactaat catttccaag gctgcggatc
     2521 atatggatgt ttcagaggtg gttattgaaa aagatctatg ggtctgctgg ttgttagagc
     2581 aattattcac tttaccagtt cggatggttt tcaaaggagg aacatcactt tccaaagtat
     2641 ttaatattat taagcgtttt tctgaagatt gtgatatcac cattgattac cgagattttg
     2701 ctgagaaaga aattgatttg gaaaaaacca gtcgctcaca gctaaaaaaa ataagtgaac
     2761 aactgaaggg tcaattgaaa cactacgtgc acgatacagt gcttccacta ctgacagaga
     2821 agcttaaaaa tcaattccct gaacaaaagt ttgagatcat attaagtgaa gatggtgaaa
     2881 cattggaatt ttattatcca agtattatga ctgattcaat tgtttatctt cgtgaccatg
     2941 tgctgatcga gtttggtatt agaaatagta cagagccttg tgaaaaacat cagattttac
     3001 cttatttaac gagttctatt ggctctgata taggtgtgcc catgccattg attaatacac
     3061 tttcgccaat acgaacattc tgggaaaaag ttacattaat ccatgttgaa tgtcatcgag
     3121 aacgattgca tcaaacgccg gagcgcttat cgagacattg gtatgaccta tacatgttaa
     3181 atcattcgca gattggggat gaggcattag agcgattgga tattttagag agtgttttgg
     3241 aatataaaag agcatttttt aacgcgagtt atgcaaatta tgatgattgt caaaagggaa
     3301 aattactcct gatcccatct tcagaaaata aagaagctct ttcaaaagac tatgcccata
     3361 tgattgatgc agatatgttc taccaaacac cgccttcatt cgcagaaatc attgtcgcac
     3421 tagaaagctt agaatttaaa ataaatcaga aggtgttggc ggtttaaaaa cctctggaaa
     3481 tttaggataa atctttatgc gcagaatctt attgatcagt gacacccatg gaaatctgga
     3541 tatcatcaat gaaaaagttg ccgaaacaat ggctgatatg gtcattcatg ctggtgactt
     3601 tggattttat gatgaacaaa gcatttatcg actgagccaa agggaattac gcttgttagt
     3661 atctcactca ccggtttggc gacaatatga agttgataaa caaacgaatc gtgaaaggtt
     3721 aatagaaata gtcaaagaac atcaattact aggtgatttt ccagattata tctcagataa
     3781 aaagaggttc ttagttccag tatatgctgt ttggggaaat catgaggatg tgcaagtttt
     3841 aaggcaatta aatgctaatc taaatgttga aaatctgcat gtactggacg aacatcactt
     3901 ctatcaattc cacaccagtg agaatgaact tgaattttct ctttatggtt tgggaggaaa
     3961 ttttttggtt ggtaaaaagt tattcgataa acccatcgca ggaaatgggg gaaaggtctg
     4021 gacagcatta catcaatttg gcgttttgta tcagcggatt aaaaataaaa gcaaaccttc
     4081 aatatttgtt tctcatgtca gtccaggtaa agagccactt ttatctcgtc taatcatgca
     4141 ttttatgcct aatttttgga tcagtggtca tatgggtgca ccgtttacct gtacttggaa
     4201 tcaatttact attagggaga tgaatgaatc cttagattgg ctagagagcg atattgattt
     4261 aatagaagaa caatatcagc aaggtcgtct tacagatgaa gccgccctgg cttatgagat
     4321 aataaaaaga cctatcgata gggatgaatc ctggtataaa aaattgtgga atattaatct
     4381 tccagatgtg gttgatggac atgcggtttt aattttggag gggaatagat tttcattgga
     4441 gacctattca aagggcatta agttcaataa ttgaatactg ctatttgcag acatagaaga
     4501 agccatctag taaggacaaa atattcttta aattgaaaac tcatcctgat tcataagcga
     4561 atctaagtgt aaggcaatcc tataaccata tagtatttat gtatacttaa ggcctatctc
     4621 gatgcgcaaa atcttattaa tcagtgattc tacagtgatg aatagaggga taaaaagtct
     4681 tggttattaa aaacccgcac gatttgagct taaagacaga ggtcttcata ggcttggcgg
     4741 gtgtgttatc tgcaattgta attagataaa tatagttttg caaatatatg caattaaatt
     4801 ccttatactt acttttaagg ttcgtccatt atggattata tgtccgtcca atatggaata
     4861 gagcttcgtc caaaatggaa tgtaaatgaa tttttccgat ttatttaatg ctcattggta
     4921 gtcatataaa aatatctggg actaatggga cacgtggggc atccatgtca ttactgggct
     4981 agacacgtc
//
LOCUS       DATNSN010000194         4872 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.195, whole genome shotgun sequence.
ACCESSION   DATNSN010000194 DATNSN010000000
VERSION     DATNSN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.195"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2810)
                     /locus_tag="VAH76_002815"
     CDS             complement(<1..2810)
                     /locus_tag="VAH76_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387398.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGDITSGSIYNGVPTIDTVAL
                     NRQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKTSGEGYQNILAAQLPMGS
                     NLSVIPNDSGLNMGLCGYWVASAGLRAHQALNQPIPPTLLNLGQTITDEMRNELDHDG
                     YRKITGWLRAVADEFPHGDEQLDAKALRENTEKDLKIEIPTLVLPGKDTSPKEAPVKP
                     TAPQDKSVPVWNGFSLYTDVTVQAAVQYAYDNYLGKPYTGPVESAPANFGGQMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPEELKKIMIAQAFFV
                     AGRDDEESEKNYAKYHQQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYAMVIEDKKKD
                     WDSTPAHVLINQGHMVDLVRVKQPPESYLESYFKSMQRWIGTQATEAVFAIQRQFFHA
                     TYEAVTGFDSDNKEPHLVTSGQFRYVIGEDGKPIREAPKMGQKEGDIKFFPPTYKLKG
                     KERFAFGNAGLGRYVIGEDGQPIRNEPAKGEKQGTLAFFPASYQLQGNERFMRVDEYL
                     KLDEVQQRFPGIGKHLEGGIPGMKYMDYMNRLNSLNRAKCETDVGFCLKQLQTAHDKA
                     KIDPVKEAFQSSKEKGRRQPNVDEIAAARIIQQILANPDCIHDDHVLINGQKLEQQFF
                     RDLLAKCEMAVVGSLLNDTDIGNIDTLMRHEKDTEFHSTGKEAIPNKIGAKWEEKQNK
                     YNASRNITETKHNLIFLMQDDSWYFSRVNAIAQNRDTGSTFKEVLITTLMTPLTSKAL
                     VDTSSAPAPKRLFRGLNFSEEYAQGLINQANTMIANTTERLFTDHSPEAFKQIKLNDL
                     SKMSGRTNASTTTNIELCRTIWDSNVIFEMLDPDDLLQPKQVGRHGAGTESEFSVYLP
                     EDVALVPVKVTLDESKTQNGENR"
     gene            complement(3130..>4872)
                     /locus_tag="VAH76_002816"
     CDS             complement(3130..>4872)
                     /locus_tag="VAH76_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387399.1"
                     /translation="QTAKLAEIKSSIEKTQVAPDLNRIIDLQSDLDAVKFTDLSPGYK
                     GFIKNTVGPVLENCLKGFMESDAVALSKVLAAFPPDGQWSAFNFEEARQAKRQMDALK
                     QMVANKVVLDALTQCQDALEKQNIAGALDALKNIPSEKEIGTIRRELREQIQSTRQEL
                     ESLQRAVVTPVVTDEKKVRERYDALIENTSKKITELETGKLPNLDAVKKGISNLSNLK
                     QEVTVLRNEKIRMHVGTDKVDFSDIEKLEQQIQVMDTKLADAYLLEVTKQISALENEK
                     PKNQTELKTKIAAFLDRTADIEAFRNDRVKKHGSSTDPLDLSDLDKLSGNLQRINQSL
                     VSSLINTIRVSISQMEAKTFHMQEKEIQQNFELLAKLEKTLDKSKTAEKLREEIPKLN
                     DLLVAKQKTYPQMVQLQLKSEVFITQLREVCQANYDDLDKTRNERLRELDRLDKEAGI
                     GRMVGNLLWGITNKVGLTTDERLDIRTKQQSLARFRTELFNDKIDTDQLISNLARKRP
                     TELQEGLGISPDNAMDLYMVLTNLESKTTSPEKLEERMKAIDDISTKIGREPEHLKFV
                     MVEEDESNKKIMGL"
BASE COUNT         1233 a         1014 c          977 g         1648 t
ORIGIN      
        1 cgattttctc cattttgcgt tttactttca tcaagggtca ctttgactgg gaccagggca
       61 acatcctctg gcaaataaac ggagaattca ctttcagtgc ctgccccatg gcgtcctact
      121 tgcttgggct gcagcaaatc atccgggtca agcatttcaa agatgacatt agaatcccag
      181 attgttctac acagttcgat attcgtggtt gtactggcat tagttctgcc ggacatttta
      241 cttaaatcat ttaacttgat ttgtttaaag gcttctggtg aatgatctgt aaatagacgc
      301 tctgtcgtat tggcgatcat tgtattggct tgattgatta atccctgagc atattcttct
      361 gaaaaattta atccacgaaa cagtcttttc ggagcagggg cacttgaggt atcgactaat
      421 gctttactgg tcaaaggcgt cattaaagta gtaatcagca cttccttgaa agtggaacct
      481 gtatctctgt tttgtgcgat ggcattgact cggctaaagt accaggaatc atcttgcatg
      541 aggaaaatta aattgtgttt cgtttcggtg atgttgcgac tagcattata tttattttgt
      601 ttctcttccc atttagctcc aattttgttt ggaattgctt cttttccagt agaatgaaac
      661 tcggtgtctt tttcatgtcg cattaaagta tcaatgtttc ctatgtcggt gtcattgagc
      721 agtgagccta cgacagccat ctcgcatttc gctaacaagt cacggaagaa ttgttgttcc
      781 agtttctggc cattgatgag cacatgatca tcatggatgc aatccggatt ggccaagatt
      841 tgctgaatga tgcgggcagc ggcaatttca tccacattgg gttgtcttct tcctttctct
      901 ttgctggatt gaaaagcctc tttaacagga tcaattttcg ccttgtcatg ggctgtttgt
      961 aattgtttca aacaaaaacc gacatcggtt tcacactttg ccctgtttaa agagttcagt
     1021 cgattcatat aatccatata cttcataccc ggtatcccac cttccagatg tttgccaatc
     1081 ccggggaatc gctgttgtac ttcatccagt ttgagatatt catcgacacg cataaagcgt
     1141 tcgtttcctt gtaactgata actagcaggg aaaaaggcta aggtaccttg tttctcacct
     1201 tttgcaggtt catttcggat aggttggcca tcttcaccga tgacataacg cccaagacct
     1261 gcattcccga atgcaaagcg ttcttttcct ttcagtttgt aagtgggggg aaaaaatttt
     1321 atatcacctt ctttttgccc cattttgggg gcttctctaa tgggcttgcc gtcttcaccg
     1381 atgacataac ggaattgacc agatgtcacc agatgaggtt ctttgttatc agagtcaaaa
     1441 cctgttactg cttcataagt ggcatggaaa aattgtcttt gtatcgcaaa aacagcttcc
     1501 gttgcctggg tgcctatcca gcgttgcatg gatttgaaat aactttccag ataggattca
     1561 ggtggttgtt tgacccgtac caaatcgacc atatggccct gattaatcaa tacatgcgct
     1621 ggagttgaat cccaatcttt ctttttatct tcaattacca tggcataaaa attgacatct
     1681 tcctgatctt tgaaaacatc aggaatcagt gtggattcat tgtctttcac atactttaag
     1741 aaggcatcac ggctttgctg atggtatttg gcataatttt tttcggattc ctcatcgtct
     1801 cttccagcta caaaaaacgc ctgggcaatc atgatttttt tcagttcttc aggggtaaca
     1861 tcagcaatag tacggccgtc tttgaatttt cctaatgttt cacctcgcaa tttggcttta
     1921 cgtgcttctt caacaatcaa ttccgcataa gccatggtcc gtaaagtatg ggaaagccca
     1981 tggtgctgtc tatacaccat ttgcccacca aaattagcgg gtgctgattc aactggtccg
     2041 gtataaggtt tgcccagata attgtcataa gcgtattgta ctgcagcttg aacggttaca
     2101 tcggtatata atgaaaaccc attccagaca ggcacagatt tatcttgcgg tgcagtgggt
     2161 ttgacaggag cttctttagg agaggtgtct tttcctggaa gaaccagggt gggaatttct
     2221 atttttaaat ctttttcagt gttttccctt aatgcttttg catctaattg ctcatcaccg
     2281 tgaggaaatt catcagcaac ggctcgtaac cagccggtaa tttttcgata accgtcatga
     2341 tcgagctcat tacgcatttc atcggtaatg gtttgaccca gattcaacaa agtaggaggg
     2401 atgggttgat ttaatgcctg gtgtgctctt aaaccggcag aggcgaccca atagccgcat
     2461 aatcccatat tcaaaccgga atcattagga ataactgaaa ggttagagcc cattggtaat
     2521 tgtgctgcta aaatgttttg atagccttca cccgaggttt tgcctaatgg gtcaaagagg
     2581 tagtattggt taccaggtcc cttgattaac ataatccagt ggccggagtc tctgtgtggt
     2641 cctactccgg ttaacacggg catgaaacct tgcctgttca gggcaactgt gtcaatagtt
     2701 gggactccgt tgtaaatgct accgctggta atatccccat accccatgcg tgcaaaaata
     2761 gcatgcatac cttcttgagt gagttctacc ccttcgacat acttaggcat tatgatttct
     2821 cccaaactga cattcataag tgtgcaaatg cgcacattgg tgaatataaa tatttcaaat
     2881 tgtatgttta ttatagaatt atagtattaa gaaagtatta aatgtaataa aaatagacat
     2941 ttggcgaatg cgataattgg aaaaacacca ggagggtgcc aagctataaa aaataacaaa
     3001 atacatacct ttgatataag ctctcaaaag acagggatca atttatggac tatatcaaaa
     3061 aaaaaaaaaa gaactgtcgg gccttattgg cccgacaaca gggattgatg ttgttatgat
     3121 aaaagcaaat taaagtccca tgattttttt attggattca tcttcttcaa ccatgacaaa
     3181 tttcaaatgc tcaggctctc tgcctatctt ggttgaaatg tcatcgattg ctttcattct
     3241 ttcttcaagt ttttctgggg aagtggtttt agactccaaa ttggttaaga ccatatataa
     3301 atccatcgca ttgtcaggag atattcccaa tccttcttgc agttcggttg gccttttcct
     3361 tgccaaatta gagattagct gatccgtatc aatcttgtca ttgaataatt cagtcctgaa
     3421 tcgtgcaagc gattgctgtt tagtcctgat gtcaagtcgt tcatctgtgg ttaaacctac
     3481 tttgttggta atgccccaga gcagattgcc caccattctc cctattccag cttctttgtc
     3541 cagccgatcc agttctcgta atctttcatt tcttgtttta tccagatcgt catagttagc
     3601 ttgacatacc tcacgtaatt gagtgatgaa gacttcactc ttgagttgca gttgcaccat
     3661 ttgaggatag gttttttgtt tggcaaccag taaatcgttt agcttgggaa tttcctccct
     3721 tagtttttct gcagtctttg acttatccaa tgttttttcg agttttgcga gtaactcgaa
     3781 attctgttgt atttcctttt cttgcatgtg aaaagttttc gcttccattt gactgatgga
     3841 aacccggata gtatttatca aagacgaaac aagggattga ttgatgcgct gtaagttgcc
     3901 acttaattta tccaaatcag agaggtccag cggatctgtt gatgatccgt gtttcttgac
     3961 tcgatcatta cggaacgcct caatatcagc ggttctgtca agaaatgctg ctatttttgt
     4021 tttcaattca gtctgatttt tgggtttttc attttcaagc gctgaaattt gtttggttac
     4081 ttcaagtaaa taagcatcgg ctaatttggt atccatgacc tgtatttgtt gttccagttt
     4141 ttcaatatct gaaaaatcaa ctttatcagt tccaacgtgc atccgtattt tttcattacg
     4201 caatacggtt acctcttgtt tcagattgct caagttagat atgcctttct tgaccgcatc
     4261 gagattgggt aactttcctg tttcaagttc agtaattttc ttcgatgtgt tttctatcag
     4321 ggcatcatag cgttctctta ctttcttttc atctgttact acaggagtaa caaccgcacg
     4381 ttgcaatgat tccagttctt gcctggtgct ctggatttgt tctcttagct ctcttctaat
     4441 ggtacctatc tctttttcag atggtatgtt tttgagagca tccagtgcgc cagcgatatt
     4501 ttgcttttcc agcgcgtcct ggcattgagt caaggcgtca agcactactt tatttgcaac
     4561 catttgtttg agggcatcca tctgtctttt ggcttgcctt gcttcttcaa aattgaaggc
     4621 tgaccattgt ccgtcgggtg gaaatgctgc taagacctta cttaaagcga ccgcatcact
     4681 ctccatgaat cccttaaggc aattttccaa aacagggcca actgtatttt taataaatcc
     4741 tttatatcca ggggataaat ctgtaaattt cactgcgtct aagtcgcttt gtaaatcgat
     4801 tattctattt aggtcaggcg ctacctgggt tttttcaata gagcttttga tttcggctaa
     4861 cttggcagtt tg
//
LOCUS       DATNSN010000195         4830 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.196, whole genome shotgun sequence.
ACCESSION   DATNSN010000195 DATNSN010000000
VERSION     DATNSN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.196"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1411
                     /locus_tag="VAH76_002817"
     CDS             <1..1411
                     /locus_tag="VAH76_002817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEO1387400.1"
                     /translation="LCNSDKTLRIDSKVLNIAESITEPEEVSIDLSNSQKKLTKKAEA
                     ELLRRKVDTVGRVNQPGEQIRNVISVAMLSEGWDAKTVTHIMGIRAFTSQLLCEQVVG
                     RGLRRMAYETNPETGLFEPEYVNIFGVPFSFLPHESTSGVPIAASPKTRIEADPKKIQ
                     YSIQWPNVVRINYNYYPQLELDFKNLSILTIDANKNIFTADMTETIGGKPDLSKATQI
                     QLEKLARTQRLQRIIFQTASSIFDQIKPHWKGMREILIAQVVMLVEKFINSDRLVIKS
                     YTGEKDSLRRRLIITLNMNEVVQHLWSEIKESNQEKLEPVFDSDHPVRSTQDMKPWYT
                     GKPCDATQRSHINFCVHDGTWESTESYELDRNQYVISWVKNDHIGFEISYTFQGIIRK
                     YRPDYLVNLINGHHLILETKGQDSSIVQAKRMAAEEWVKAINQQEGFGVWHYAISFNP
                     KEIKSILHKLVTVDPQSNN"
     gene            complement(1700..2065)
                     /locus_tag="VAH76_002818"
     CDS             complement(1700..2065)
                     /locus_tag="VAH76_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HEO1387401.1"
                     /translation="MKKSLTENIMTSQQSQQRTNARKNKVAFLAMREDISEALKKGWS
                     ITVIWETLRDEGSFTATYNTFRLYVLKYLKGQKSGYSLKDTNVQQPKRLAVNQDKEKK
                     PTAMPSFTFNPIPNLKDLL"
     gene            complement(2393..4144)
                     /locus_tag="VAH76_002819"
     CDS             complement(2393..4144)
                     /locus_tag="VAH76_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEO1387402.1"
                     /translation="MSQNHVIELPNTPKQRPVFCDYAGGRFRLTAEGLTFIGIDKDGN
                     PLPPRWICAPLYVVAKTRDAQSGEWGRLLEWKDDDGITHQWAMPLALLQGDASDVRRE
                     LARLGLSISPNRTARDLLASYLQVFPVDARARCVDKLGWHGDVFVTATQCIGNSTEKI
                     VFQNTNTIEPAVSVKGSVEEWRDSIGRLASGNSRLVFAISVALAPVLAKIVGEDSGGF
                     HFRGASSSGKSTALSLAGSVWGNPQSYCRLWRSTTNGLEGLAALHNDGLLILDELSQM
                     DPREAGEAAYLLANGQGKTRASRTGTVRQSSRWSLFFLSAGEESLTALMAKSGQRINA
                     GQEIRLADIEADAGCQMGIFETIHDQLSPASMALSLKKYSSRYHGAIGLAWLNQVVAN
                     RKTISRYITDTIQTFIDSVIQPDATGQIIRVARRFALVAAAGELASQFGLTGWQKGES
                     FHAAKTCFMAWQDTFGVDGHHEDRAIMAQVRAFFESHGASRFDSANSPNNDKILNRAG
                     FYHTDGEGFRIYMVLTEAYKNELCKGFDQRTVTRVLLQAGWLKPASDGKASHKPRIKG
                     VGTPRLYVFTGKIWGGE"
     gene            complement(4209..4484)
                     /locus_tag="VAH76_002820"
     CDS             complement(4209..4484)
                     /locus_tag="VAH76_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO1387403.1"
                     /translation="MTLKKPSRLHLLNEFESAPHSALFNQQTIAAVLSCSTQLLERNR
                     WAGGGVPYLKIGRKVLYRKSDVLSFLQQQKIYYSTSDEGQLQLVENA"
BASE COUNT         1473 a         1016 c          934 g         1407 t
ORIGIN      
        1 attatgtaat tcagataaaa ccttacgtat agattccaaa gtattaaata tagctgaatc
       61 tattactgaa ccagaagaag tatcaattga tttatctaac tctcaaaaga agttaacaaa
      121 gaaggctgaa gctgaattgt tacgtagaaa agtggatact gttggtcggg taaatcagcc
      181 aggagaacaa atccgtaatg ttatctcagt cgcaatgcta tcagaaggat gggatgctaa
      241 aacagtaacc catattatgg ggatacgtgc atttacaagc cagctccttt gcgaacaagt
      301 ggttgggcgt ggattgcgac gaatggcata tgaaactaat ccagaaacag ggttatttga
      361 gccagaatat gttaatattt ttggggttcc attctcattt cttccacatg aatcaacctc
      421 aggagttcca atagctgcct cacccaaaac acgcatagag gctgatccaa aaaaaattca
      481 atattcgatt caatggccta atgttgttcg tataaattac aattattatc cacaattaga
      541 attagatttt aaaaatcttt caattttaac catagatgca aacaagaata tatttactgc
      601 agatatgacc gaaacaattg gaggaaaacc tgatctttca aaagcaactc aaatccagtt
      661 agagaaacta gctcgtactc aacgcttaca aagaatcatt tttcagacag caagcagtat
      721 atttgaccaa atcaagcctc actggaaagg tatgcgcgaa atactcattg cacaagtagt
      781 aatgttagtg gaaaaattta taaattctga ccgattggtc attaaatcat acactggcga
      841 gaaagatagt cttaggcgac gtttgattat aactctaaat atgaatgaag tagtacagca
      901 tctttggagt gaaataaaag aatctaacca agaaaaacta gaaccagttt ttgatagtga
      961 ccatcccgtg cgtagtacgc aagatatgaa accttggtat acaggaaaac catgcgatgc
     1021 aactcaacga tcacatataa atttctgcgt gcatgatgga acttgggaat caacagaatc
     1081 ctacgagtta gatagaaatc aatatgtaat ttcctgggtt aaaaatgacc atattggatt
     1141 tgagatatca tatacttttc aaggaataat aaggaaatac cgaccagatt atttagttaa
     1201 tttaataaat ggtcatcatt taatattaga aactaaaggt caagactcat ctatagtaca
     1261 agcaaaaagg atggctgctg aggaatgggt aaaagctatc aatcagcaag agggatttgg
     1321 tgtatggcat tatgctattt ccttcaaccc taaggagatt aaatcaattc tacataaatt
     1381 ggttacagtc gacccgcaat caaataatta aaaaaaggaa acaataattt ttttctgctc
     1441 tttaccaagc ctcacatata cttttgctat acccgctaaa tcatcatagt cagcataaaa
     1501 aaatcatggt catgctcgat ttattaacat ttatcatcaa gaaaataaat tcacataaac
     1561 atgttatcca atatctcagg gacatagggg acatttgggg cagacacttt aatactgggg
     1621 gttagaagtc cccacctgtg tgacgaaggt ggggcatgtg ggacttattg aactatatcc
     1681 accaataaaa tccattccat cataacagat cctttaaatt gggtatggga ttaaaggtaa
     1741 atgaaggcat agcggttggc tttttctctt tgtcctgatt tacggccagt ctcttcggct
     1801 gctgaacatt ggtgtccttg agcgaataac cggacttttg gcccttcagg tatttcaata
     1861 cgtataacct gaacgtattg taagttgctg tgaagctccc ctcatcccta agcgtttccc
     1921 atatgacggt aattgaccat ccttttttca aggcttcgga aatgtcttcg cgcatagcca
     1981 ggaatgccac tttatttttc ctggcgttcg ttctttgttg actctgttga gaggtcatta
     2041 tgttttctgt taaggatttt ttcatggttt acactccata ttattggtta ttcaatcctt
     2101 tgagttttat cgtttatttc tatttatttt cgacctttta ggtcgctggc aaagcccttt
     2161 tgggtaattt taggtaattt attatttatt tctgtttatt cttgattatt ttcgttacta
     2221 atcgttatat aacgaacaat aacgcataat taccaaaaga cggttccaaa ataaatttca
     2281 aaatgcaggg caagataggt gaagttagct aaccagcgag ctggttgcca aacttcacga
     2341 gtcttattcg caccttcggt gctcaacgta aaaaccgatg atataagcaa ttttattcac
     2401 ctccccaaat tttaccggta aaaacataca gtcttggtgt gccaactcct ttgattcttg
     2461 gtttgtgtga ggctttgcca tcagaggcag gtttcagcca gccagcttgt aataaaaccc
     2521 ttgtcactgt gcgttgatca aagcctttgc acagctcatt tttgtaggct tcagttaaca
     2581 ccatgtagat tcgaaatcct tcgccatctg tatgataaaa tcctgcccgg ttgagaatct
     2641 tgtcattgtt gggtgagttg gcactatcaa aacggctggc tccatgagat tcaaagaacg
     2701 cacgtacttg cgccataatg gcgcgatctt cgtgatgacc atcaacccca aaagtatcct
     2761 gccatgccat aaagcaagtc ttcgcagcat ggaacgattc tcctttttgc catcctgtca
     2821 gaccaaattg actagccagt tcaccggcag cagcaaccag ggcaaagcgt ctggcaaccc
     2881 gaataatctg cccggttgca tcgggttgaa tgactgaatc aataaaggtt tggatggtat
     2941 cggtaatata tcggctgatt gtcttcctgt tagcaactac ttgattcagc cacgccagac
     3001 cgattgcgcc atgatatcga ctgctgtatt tcttcagcga taatgccatg ctggctgggc
     3061 ttagttgatc gtgaatcgtt tcaaagatcc ccatctgaca gcctgcatct gcttcaatgt
     3121 ctgcaagcct gatttcctga ccggcattaa tacgctgtcc tgattttgcc atgagtgccg
     3181 tcaaagactc ttcaccagca gataagaaaa atagtgacca tcgagatgat tgcctgacag
     3241 tgccagttcg cgaggcacgt gtttttccct gaccattggc tagcaaataa gcggcttccc
     3301 ctgcttctct tggatccatt tgactgagtt catcaagaat gagcaagccg tcattgtgta
     3361 atgcggccaa gccttcaaga ccattggtag tactgcgcca taaacgacaa taagattgtg
     3421 ggtttcccca aacagagcca gcgaggctta atgcggtgct tttgcctgat gaggatgcgc
     3481 ctctgaaatg aaaaccgcct gagtcttcgc caacaatctt tgccagaact ggagcaagtg
     3541 ctaccgagat ggcaaatacc agtcttgagt tgccggaagc cagacgacct attgagtcgc
     3601 gccattcttc aacactgccc ttaacggata cagcaggctc aatagtattg gtattttgga
     3661 atacaatctt ttctgttgag ttcccaatac attgagtcgc tgttacaaag acatccccat
     3721 gccaacccaa tttatcaaca catcgtgcgc gtgcgtctac tggaaagact tgtaaataag
     3781 aggctaacaa gtcacgagct gttctattag gcgaaatact caatcccaat cgtgccaact
     3841 ctcgcctcac atcggaggca tcaccctgca acaaagccaa gggcattgcc cactgatgag
     3901 ttataccatc atcatccttc cattccagca aacgacccca ttcaccactt tgggcatctc
     3961 gggtctttgc aacgacatac aatggcgcac atatccaccg aggtggcaat ggattgccat
     4021 ccttatcaat accgataaag gttaatcctt cagcagttaa ccgaaatcga ccacctgcat
     4081 agtcgcaaaa aactggacgc tgctttggtg tattgggtaa ttcaataaca tgattttgtg
     4141 acatacatat tctccaaaca acagtaaaac catcggaatg aaacaaattc attctgacga
     4201 atttaaattt atgcattctc aacgagctgt aactgacctt cgtcactggt tgagtaataa
     4261 attttttgtt gttggagaaa actcaacaca tcgctttttc ggtacaacac cttgcgacca
     4321 atttttaaat agggaacacc accacccgcc cagcggttac gttcaagtaa ctgggtggag
     4381 cagcttaata ccgcagcaat cgtttgttga ttaaaaagcg cagaatgtgg cgcagattca
     4441 aattcgttta atagatgtaa acgggatggt ttttttaaag tcataggaaa ttcctcggtt
     4501 atttacacct cagccaaatg ccgaggggta tcttccttga acagaactca acgcatcatc
     4561 cagtatcacc ctggacaatt gattgatatt cttttctcct aatcctttga tttccaactg
     4621 cctacatttt gttggaacat cgggcttact ccttaagctt ggaaaaagta tctcgtattc
     4681 cctcaaagaa tgtcaagcac aaaagatcaa attcaaagat gtgttatcga aggatattga
     4741 ttgatactaa aaatgaaata aaacatgtca taaattcgta attcgcgaat cgcgaattta
     4801 tcataacttt tagttttatt acttaagtgc
//
LOCUS       DATNSN010000196         4813 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.197, whole genome shotgun sequence.
ACCESSION   DATNSN010000196 DATNSN010000000
VERSION     DATNSN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.197"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..395
                     /locus_tag="VAH76_002821"
     CDS             <1..395
                     /locus_tag="VAH76_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387404.1"
                     /translation="LLELSKINCAVADNMTQTQLGLDLIDSYEKFLEITKINAKVAFN
                     FQILNYQRYAKLVDAYQQSSQAKSLSGLNTSANSQLFFKKRDSTGQDRASITTGQASI
                     AHDTVGLGLEWNEAIQNTISENMMNQKL"
     gene            454..1320
                     /locus_tag="VAH76_002822"
     CDS             454..1320
                     /locus_tag="VAH76_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO1387405.1"
                     /translation="MKNRRDEKKMLALIIKIANDDERIKIVIMNGSRASPSAKKDIFQ
                     DYDIVYLVTEIESFVHDKNWINQFGELLIMQTPDEMDGQWPKFKGKYTYLMQFKDWNR
                     IDLTLLHIDQLETMPRDSQSLLLLDKDNLIAPFGEPTDEDYLPSPPTEKNFVNCCNEF
                     LWVSTYVAKGLWRKQLIYAKHVSEQIVKEELIKMLMWYAGIQTNFSQPMGTYGKYIEN
                     HLELELWQEFLKTYVDADYKHMWASLFKMCEIFNDLAIKISSYFGYSFNQKEFDNVVD
                     YLQDIKNNKIPH"
     gene            1453..3249
                     /locus_tag="VAH76_002823"
     CDS             1453..3249
                     /locus_tag="VAH76_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1387406.1"
                     /translation="MPKTPNNCSLSSGNKPPTLSRILFSYMAEYRWSTAFFILFGFFG
                     AFTLPYMSYLLGQIIDEIKFQNPEQVDIFSLVLTSLILYVAIHVLRSLGYYVHGLLSL
                     TSIPEKKAKLIKQLFNHLGNQSIGYFEDKHSGYLTNKITNASSSIEPLIFNLFTIIYP
                     QSLAIILTGILLSLVVPYFGVVLWLWGAALIIYSYHSAKIGNDKAMLLANSNSKVNGK
                     VVDVVNNIHTVIYNAEMSFELGNLDTDIQDMAQNDRNLQRHMTKVMLVQHIAMNILVA
                     FFLIGSVLGYNYGYVSVGDIVFVMTSVTAIAGLTSSLGKCFLDFVTNAGRLNEGLSLF
                     GDQHTITDNANAQAHQINNGEIQFHDISFAYPNQPPIFLNFNLTIPAKQKIGIVGSSG
                     AGKTTLFKLLMRLYNVEKGVIAIDGLPINAYTKKSLCSQIAIVPQHLILFHRSIYDNI
                     TYGCNNFDKSDVILAAKKAHCHEFIAELPEQYDTIIGEQGVKLSGGQRQRIAFARAIL
                     KNAPILLLDEATSALDSRTEQAIQDGLQNLLADKTALVIAHRLSTLKAMDRILVLEDG
                     KIIENGTHTELLEKRGAYYNYWSHQSEGFIRA"
     gene            3537..>4813
                     /locus_tag="VAH76_002824"
     CDS             3537..>4813
                     /locus_tag="VAH76_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387407.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHGDITSGSIYNGVPTIDTDAL
                     NRQGFMPVLTGVGPRRDSGHWIMLIKGPGNQYFLFDPLGKTSGEGYKNTLLAQLPIAS
                     TLSVIPNNPGLNMGLCGYWVASVGLKARAELNKDNPPDLETLGRTTTEEMRNELTDNG
                     YLKITGWLRAVADNFPAGAPQPDAKALRETTEKDLHIELPSPVPPVKDTAPKEVSTKP
                     TAPQIAPKHSLDSKLLENDDDVLDTIKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEG
                     PDRGPHGLAHTVRTMACAEVMIEEARKAQLRGETLGKAKNGQTLADVTPEELKKILIA
                     QAFFVVGRDDERSGYDDVHKRNFYAEYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFY
                     AAIILDKNHEWDASPAHILINQGHMVDLMRTKAP"
BASE COUNT         1631 a          894 c          904 g         1384 t
ORIGIN      
        1 aactattaga actctctaaa attaactgtg ctgttgcaga taatatgaca caaactcaat
       61 tgggtctgga tttaattgat agctatgaaa aatttctgga aataacaaag ataaatgcta
      121 aagttgcatt taattttcaa atattaaatt atcaaaggta tgccaaactg gtcgacgcat
      181 atcaacaatc ttcacaagca aaatctcttt caggcctaaa tacctcggca aattcccaac
      241 ttttttttaa gaaacgcgat tctactggac aagaccgagc ttctattacc acgggacaag
      301 cgagtatagc tcatgataca gttggattgg ggctggaatg gaacgaagct atccagaata
      361 ccattagcga gaacatgatg aaccagaaac tttaacagca aaccctaata gctattctag
      421 aatttaaatt taaaaaggcc ataatccaga aatatgaaaa atcgacgtga tgaaaaaaaa
      481 atgcttgcac tcatcatcaa aatagctaat gacgatgaga ggattaaaat tgttattatg
      541 aatggctctc gagcaagccc ttcagcgaag aaggatattt ttcaggatta tgatattgtg
      601 tatctggtaa ctgaaataga atcatttgtt catgataaaa attggattaa tcagtttggt
      661 gaattactta tcatgcaaac acctgatgaa atggatgggc aatggccaaa atttaaaggt
      721 aaatatactt atctgatgca atttaaagat tggaacagga ttgatttaac cttgttacac
      781 atcgatcaac tggaaactat gcctagagac agtcaaagtc tcttattact tgataaagac
      841 aatttaattg caccttttgg cgaacctact gatgaagatt acttaccaag cccccctaca
      901 gaaaaaaatt ttgttaactg ttgtaatgaa ttcttatggg taagcactta cgttgccaaa
      961 ggactctgga gaaaacaact catctacgca aaacacgttt cagagcagat agttaaagaa
     1021 gagttaatta aaatgctcat gtggtatgca ggaattcaaa ccaattttag tcaaccgatg
     1081 ggaacttatg gaaaatatat tgaaaaccat cttgagctcg agctctggca agaattcctc
     1141 aaaacctacg ttgatgctga ttataagcac atgtgggctt cactattcaa aatgtgtgaa
     1201 atattcaatg acctagcgat aaaaatttca agttattttg gttactcatt taatcagaaa
     1261 gaattcgata atgtggtaga ttatttgcaa gatataaaaa acaataaaat tccacattaa
     1321 tcgaacaaat ccacttggaa ttaaagaata aatactattg agatatcgat gttggttatc
     1381 gatttttgca aatgaattga gagcactccg ccatatgagg tggaacaaat tttaaagttt
     1441 gagggaaatg ttatgcctaa aactccaaac aattgtagtt tatccagcgg taataaacca
     1501 ccaaccctct caagaatctt gttctcatat atggctgaat accgctggag tacagcattt
     1561 tttattttat ttggcttttt tggggcattt actctacctt atatgtccta cttacttggg
     1621 caaatcatag atgaaataaa atttcaaaat cctgaacagg ttgacatctt tagcttagtc
     1681 ctaacctcat taattcttta tgttgcaatt catgttttgc gaagcttggg atattatgtt
     1741 catggtttgc tttcacttac cagtattccc gaaaaaaaag ccaaactgat taaacaacta
     1801 tttaatcacc tgggcaatca atcgatcggt tattttgaag ataagcactc cggttattta
     1861 acaaacaaaa ttaccaatgc cagttctagt attgagccac ttatttttaa tttatttacc
     1921 attatttacc cacaatcatt agcaattatc ctaacaggta tattattgtc tttagtcgtt
     1981 ccgtattttg gagtggttct ctggctctgg ggagctgcac tgattattta ttcataccat
     2041 tccgccaaaa ttggtaatga taaagcaatg ctccttgcga attccaatag taaagtcaac
     2101 ggtaaggtag tcgatgtggt caataacatc cacacagtaa tctacaatgc tgaaatgtct
     2161 tttgaacttg gtaatctgga tacagacatt caggatatgg ctcaaaatga tcgcaatttg
     2221 cagcgccaca tgacaaaagt catgctggta cagcacattg caatgaatat cctcgttgct
     2281 ttctttttaa taggctcagt gcttgggtat aattatggtt atgtcagtgt aggcgacatt
     2341 gtttttgtta tgacatcagt tactgctatt gcaggcctca caagcagcct tgggaaatgc
     2401 tttctcgatt ttgttactaa tgccgggcgt ttaaatgaag ggttgagctt attcggagat
     2461 caacatacga ttactgataa cgcaaatgct caagcacatc aaattaataa cggtgagatt
     2521 caatttcatg acatttcatt tgcttatccc aatcaaccgc caatttttct aaattttaat
     2581 ttaacgattc cagcaaagca gaaaatagga attgtaggta gttcaggggc tggtaaaacg
     2641 acattattca agttattgat gcgattatat aatgtagaaa aaggtgtcat tgcaatagat
     2701 ggcttgccaa taaatgccta cactaaaaaa tcactttgct ctcagatagc aatagtgcca
     2761 caacatttaa tactttttca tcgtagcatt tatgataata tcacctacgg ttgcaataat
     2821 tttgataaat cagatgtgat tttagctgca aagaaagcac actgtcatga atttatcgct
     2881 gaactgcctg agcagtatga caccattatt ggcgagcaag gtgtaaaatt atcaggtgga
     2941 caaagacagc gtattgcatt tgcccgagct attctcaaaa atgcgcctat cttgttactt
     3001 gatgaagcga catcagcctt agactccagg actgaacagg caattcaaga tggcttacaa
     3061 aatttgttag cagataaaac tgcgttagtg attgcgcacc gtctatcaac gctcaaagca
     3121 atggatagaa ttctagtttt agaagatggc aagatcatcg agaatggaac acataccgaa
     3181 ttattggaaa aaagaggagc gtattataac tactggtcac accaatcgga aggtttcata
     3241 cgagcatgaa acaatctgct aactgcctgt aaactgttaa tataatctta agaaaaattt
     3301 ataaatatta gaactttaaa ctcttatatc atcgtatcaa tctacctcgc ttatttttga
     3361 aacagaaagt cccaactgtc tctcaatttc acgaacacaa aaaatcaaga gcctgatttt
     3421 aaactcttaa tatttcctta aaactattat cctataataa atataccaag tgtgaatatt
     3481 cgtatccatt agaatcagta tttgcatgta caaacatcag tttgggagaa agtaaaatgc
     3541 ctaaatacgt agaaggggta gaattaactc aagaaggcat gcatgctatt tttgctcgta
     3601 tggggcatgg cgatattacc agcggtagca tttataatgg agtaccgact attgacacag
     3661 atgccctgaa caggcaaggc tttatgcctg tattaacagg ggtaggcccc agaagagact
     3721 ccggtcactg gatcatgtta atcaaagggc ctggtaatca atactttctc tttgacccac
     3781 taggtaaaac atcaggcgaa ggttataaaa atactttatt agcccaatta cccatagcat
     3841 ctactctttc tgttattccc aataaccccg gcctaaacat gggactatgc gggtattggg
     3901 ttgcctctgt cgggctaaaa gctcgtgctg aactgaacaa ggacaatcct cctgatttag
     3961 aaaccctggg gcgaaccacg acagaggaaa tgagaaatga attaaccgat aacggttatc
     4021 tgaaaattac agggtggtta cgtgctgtgg ctgataactt tccagcaggc gccccccaac
     4081 ctgatgcgaa agctttaaga gaaactaccg aaaaagattt acacatagag ctcccctccc
     4141 ctgttcctcc agtgaaagac acagctccta aggaagtttc tacaaaacct actgcaccac
     4201 aaatagctcc caagcattct ttggacagta agttattaga gaatgacgat gatgttctag
     4261 acaccataaa gtatgttcat aaagagtatt taggaaaacc atatcctggc ccattaaaaa
     4321 atccaaaagc accggaagaa ggacgacttc ctccaaatga agggcctgat cgaggacctc
     4381 atggtcttgc tcatacggta agaacaatgg cttgtgctga ggtgatgata gaagaagcac
     4441 gcaaagctca actgagagga gagacgttag gtaaagcaaa aaatggtcag actttagctg
     4501 atgtaactcc agaggaactg aaaaaaattc tcatcgcaca agcctttttt gtggtaggaa
     4561 gagatgatga gcgttccggc tatgatgacg tacacaagag aaatttctac gcggaatatc
     4621 atgagaaaag tgagcaagcg ttcaggaaat acgttgaaga taacaagctc atcggcaaaa
     4681 tctttaaaga tcaaaaagaa gtcgatttct atgccgcgat tattctggat aaaaaccatg
     4741 aatgggatgc ctccccagcg catatcctga taaatcaagg ccatatggta gacttaatga
     4801 gaactaaagc acc
//
LOCUS       DATNSN010000197         4812 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.198, whole genome shotgun sequence.
ACCESSION   DATNSN010000197 DATNSN010000000
VERSION     DATNSN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.198"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(5..691)
                     /locus_tag="VAH76_002825"
     CDS             complement(5..691)
                     /locus_tag="VAH76_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO1387408.1"
                     /translation="MSKFIFVIGGTGTGKTTASEEIKTIAEAQNFTVTILSLDHYYLP
                     QSMMDPDKPKNFDVPEALEQTLIQQHLQDLEAGKTIFRPTYDMASSDRVLNGEMKIIP
                     ADIIIVEGIFAGEYLSSLRNQTEKLKIYLQSNQINDNYTRKEDRDIVERKKSSAHAHV
                     AKKNQMAGFFRYVATHMISSNMVIDNPWLPQQSMTTTEKLPLIAGNNLIKLNEFLSST
                     ESHFLYKTYS"
     gene            complement(1047..2201)
                     /gene="argE"
                     /locus_tag="VAH76_002826"
     CDS             complement(1047..2201)
                     /gene="argE"
                     /locus_tag="VAH76_002826"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEO1387409.1"
                     /translation="MTTLDWLKQLISFDTTSRNSNLSLIEYLANGLNDFKINPILIHD
                     NKEQKANLLATLPGKQGRLEGGIILSGHTDVVPVDGQIWDSDPFQATLKNNKVYGRGA
                     CDMKGFIAVVMALVPQLKEMNLDFPVHFAFSYDEEIGCLGVPSLIDKIVELNYQPRAC
                     IVGEPTLMKPVVGHKGKYSYRCQIHGVAAHSSLTNQGCNAIEHAASFISYLRGMANEF
                     KKYGNRDFSYDVPYTTLTTNLIKGGNAYNTIPNLCEFVFEFRNLSTDSSEDLNQKIMS
                     YVKEQLVPNLHQDHPDSNVNLDTIAKAPGLDMPITDPFVRAAQTVCQSDKLMKVAYAT
                     EAGLFQQAEIPTIVCGPGSIEQAHRANEYVEVEQLRLCEEFIINMLRSHF"
     gene            complement(2213..3499)
                     /locus_tag="VAH76_002827"
     CDS             complement(2213..3499)
                     /locus_tag="VAH76_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387410.1"
                     /translation="MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHS
                     PVTSLLATMASSGVALVVRPLGGFILAVWADKYGHHKALMFVLYLMAVATMLITFTPS
                     YSTIGLAATCIIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
                     LMGSSACLILVFYCSNETLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDT
                     GKLLILVKQQKRSLLIAFSLVAGCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGI
                     LVSILTIPLFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQYPGVTILILVENILG
                     LCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNLAVLLFGGFAPFIITALIEYTKNPMV
                     FTYYQMTTVFISLLAVLSYQEERKLSENQPEHLVAI"
     gene            3670..4401
                     /locus_tag="VAH76_002828"
     CDS             3670..4401
                     /locus_tag="VAH76_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1387411.1"
                     /translation="MKKFILFPLISALSSTFVHAGGMGDSNSCCSTFVSLEGGYTWSS
                     IDGYNFTIVGTNNTLTSTEDKQGYSGRLAVGVLSMIDDQYGFTGEVGWGYYGRTTINP
                     SVAGALGQVPAALTIKHTLSGFDALLGVTYFQNYFSWSAKAGALIQNMQVDTSAFFTP
                     QIFPIVDNFDMKTNRTAVLPAAKLGVAYNFDSNWAITGSYLFALGANPGTTATFNPNT
                     LRASLSIDDENPMMNAILFGVQYTA"
BASE COUNT         1576 a          941 c          840 g         1455 t
ORIGIN      
        1 cattttaact atatgtttta tataaaaaat gactctcagt tgaagataaa aattcattta
       61 attttattaa gttattgcct gcaatcaaag gaagtttttc ggtagtagtc atgctttgtt
      121 gaggaagcca aggattatca atgaccatat ttgaagaaat catatgggta gcgacatatc
      181 taaaaaatcc tgccatttga ttctttttcg caacatgggc atgagctgat gatttttttc
      241 tttcaactat atctctgtct tccttccttg tgtaattgtc atttatttga ttggattgta
      301 aataaatttt gagtttttct gtttgattcc ttaaagagga taaatactca ccagcaaaaa
      361 ttccttcgac aataatgata tcagcaggaa taattttcat ttccccattc aataccctgt
      421 cactgcttgc catatcatag gtcggtctga atatggtttt accagcctct aaatcctgca
      481 aatgctgttg aattaatgtt tgttccagag cttcagggac atcaaaattc tttggcttgt
      541 ccggatccat catactttgg ggcaagtagt aatgatccag acttaatatc gtaaccgtga
      601 aattttgtgc ttctgctatt gtcttaattt cttctgaagc agtcgttttt ccagttccag
      661 ttcctccaat gacaaagata aatttagaca cgtaatattc tcttgcggtc aaaaaaggca
      721 ttataataac tgaggaacaa attacaagag aaccaatcaa tctgtacctt gataagctct
      781 cgagccaaat ccagtattcc aaagcttata aagcgaacca ctttaaagat tgtagataaa
      841 ataaaatgat tacggcaaat gctggcataa aaaacattta ttttaataac caattgataa
      901 aaaagtattt ttctttaaaa ccaatcaaat tttatctaat catgttatcc acaaaaactg
      961 tgtatatctt tgtgaatacc ttataaaagt gctagtaaaa tcagcatcaa ataccgatga
     1021 ttttttactt accaatctga taaagctcaa aaatgagagc gcagcatatt gattataaat
     1081 tcctcacata atcgtaattg ctctacttca acatattcat ttgctctatg ggcttgttcg
     1141 atactacctg gaccacacac aatagtggga atttctgcct gttgaaatag cccggcttct
     1201 gtagcgtaag ctactttcat cagtttatcg ctctggcaaa ctgtttgagc tgctctgaca
     1261 aaaggatctg taatcggcat atccaaacca ggagcttttg ctatggtatc aagattaaca
     1321 ttagagtcag gatgatcctg atgcaggtta ggaacaagct gttccttaac ataagacata
     1381 attttctgat ttaaatcctc agagctatca gtagacagat ttctaaactc aaaaacaaac
     1441 tcacacaagt ttggaatagt attataagca ttacctcctt tgatcagatt ggtggtaagt
     1501 gttgtatatg gaacatcata agaaaaatct cgatttccat attttttaaa ttcattagcc
     1561 atccctcgaa gatagcttat aaaagacgcg gcatgttcga tcgcattaca tccttgattc
     1621 gttaatgaag agtgagcagc tacaccatgt atttggcagc gataggaata tttgccttta
     1681 tgacccacaa caggcttcat taaggttggc tcacctacaa tgcaggcacg cggttgataa
     1741 ttaagctcca ctattttatc gatcagagaa ggtacaccaa gacaaccaat ttcctcatca
     1801 taagaaaatg caaaatgaac tggaaagtcc agattcattt ctttcaattg tgggactaaa
     1861 gccatcacga ctgcaataaa tcctttcata tcacacgcgc cacgaccata cactttgtta
     1921 tttttaagag tggcttgaaa aggatcacta tcccaaattt gaccgtcaac aggaacaaca
     1981 tcagtatgac cagacagaat aataccccct tccaaacgcc cttgttttcc tggcaaagta
     2041 gctaacagat tagctttctg ctctttatta tcgtgaatta ggataggatt aatcttgaaa
     2101 tcatttaacc cattcgccaa atattcaatc aatgataaat tagagttccg cgaagtagta
     2161 tcaaaagaaa tgagttgttt taaccaatct aaagttgtca tattcctacc ttttaaattg
     2221 caacgagatg ttctggctga ttctcagata gcttcctttc ctcctgataa gaaaggactg
     2281 ctagcaaact gataaagacc gtcgtcattt gataataagt aaaaaccata gggtttttag
     2341 tgtattcaat caatgccgtt ataataaatg gagcaaaacc accaaacaac aggacagcta
     2401 aattatagct gacggctaaa caagtagaac gtactgatgg tggaaataag gagctaaccg
     2461 taatggtcaa cactccaaaa taagcgccga tacacaaacc taaaatgttt tcaaccaaaa
     2521 tgagaatagt tacaccaggg tattggttga gcaagaaaag taaaggataa attaatccaa
     2581 aataacaggc caaagcacat aacataacta tttttttgcc tattttatcc gacaatgcgc
     2641 caaaaagagg aatagtaaga atactgacta atattcctga tgcagtgcct aagtacgcct
     2701 ctgtcagcga taaattcaaa tacaatttac aataggtagg catatagctg aataaggtat
     2761 aaatcgagcc tgtacacccg gctacaagac taaaagctat caacagcgaa cgtttttgtt
     2821 gctttaccaa gataagtaac tttcctgtgt cctgttgctc tttcttccaa gtaaactctc
     2881 gttgcaatcg acgtcttaaa atccatccta aaggtagaat caacagtcct agagcaaaag
     2941 gtattctcca ggcaaattga tatagggttt cattggaaca atagaaaact aaaataaggc
     3001 acgctgaaga tcccattaat attgccaaaa gctgacctac catttgtaag gaagtgtaaa
     3061 accccttttt atcacttggc gataagctaa ctaatagagc gctggatatg ccaaactcgc
     3121 caccagtagc aagcccttgc aacaatcttg caaatacaat gatacaagta gccgccagac
     3181 ctattgtgga gtaggatggt gtaaaagtaa taagcatggt tgcgacagcc atcaaatata
     3241 aaacgaacat taatgcttta tgatgcccat acttatctgc ccaaactgct aaaataaaac
     3301 caccaagagg ccggactacc agagccactc cggagcttgc cattgtagcc aagagggaag
     3361 tcacaggact atgattggga aaaaaagctt gggcaataaa aagcagcatg aagttataaa
     3421 tcatgaaatc ataccattcc atagcattac caatacatat tgctcccaat gttttaaagg
     3481 aggtatttga tgtattcatg tttccttacg agggtaaatt tagagcaaag cacatactag
     3541 tagaatataa aaaaaattac catgctttat aagaaactaa catcaatatt aattttactg
     3601 gtaattccct atcaactgat ttaaattatt gacttttaca ataataaaca tggaatgagt
     3661 tactaaatca tgaaaaaatt tatcctgttc cctttaattt ccgccctgtc cagtactttt
     3721 gtccatgctg gtggtatggg tgattccaac tcttgttgct ctacttttgt gtcacttgag
     3781 ggtggataca cctggagttc cattgatgga tacaatttta ctatagtcgg taccaacaat
     3841 actctcacct cgacagaaga taaacaaggt tatagtggcc gtctggctgt cggtgtgctt
     3901 agtatgatag atgatcaata tggattcaca ggcgaagtag gctggggtta ctatggtaga
     3961 acgaccatta atccaagtgt tgcaggagct ttaggacagg ttccagctgc cttgacgatc
     4021 aagcatacct tgagcggatt tgatgccctg ctcggcgtta cctattttca aaactatttt
     4081 agctggtctg ccaaggcggg cgcgctcatt caaaatatgc aagtagacac tagtgccttt
     4141 ttcactcccc aaatatttcc tatagtggat aatttcgata tgaaaacgaa caggactgct
     4201 gttttgccag cagcgaagct gggagttgcc tataactttg attctaactg ggctattacc
     4261 ggctcttatt tgttcgcgct tggagcaaat ccaggaacaa ctgcgacttt caatcctaac
     4321 acattaagag ccagtctgag cattgacgat gaaaatccca tgatgaatgc tattttattt
     4381 ggggttcaat atactgctta gttcgatatt atttaccccc cttttacatg aggtcgcatg
     4441 tttacaaata gataaaagcc tttaaggttt ttgaaatttg agaaaacatg ctgacctcaa
     4501 taaggtaatg gtaaggaccc ttccactttc aagatgttcc caattgagcc aaaatgaaat
     4561 tcctgtagcg tcatcctccc atatcctggt tagtcattaa aagattttat tgatttttat
     4621 tcaattttta attacctatt gttacaactc aagagctgca ttaagacact cttcgggaca
     4681 tgccagcacc atcggtaaga acttgcttct ttctaattaa gcaggagaca gctcaaaacc
     4741 aatacaatca aattgcatat ttttatcaaa ataatgtata atgcgccaat tagtatattt
     4801 tttgagctga at
//
LOCUS       DATNSN010000198         4774 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.199, whole genome shotgun sequence.
ACCESSION   DATNSN010000198 DATNSN010000000
VERSION     DATNSN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.199"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            63..1544
                     /locus_tag="VAH76_002829"
     CDS             63..1544
                     /locus_tag="VAH76_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEO1387412.1"
                     /translation="MNAYQQLEQHFKKYYDFENLAAIVSWDEATMMPAGGGQARAEAL
                     ATLSAVQHEWLTNKKVGDWIKQAKDLPGLSLWQRRNIYWIDKQYQQATCIPAQLIEEF
                     TNESLICVQAWRELRAKNDWRSFKPYFKKLFEKVKMIADIKSQRFNKPCLDVLIDEYS
                     PDLDCKAITPVFDVLKQKIPAIIPNVIEKQKRRDIKVISGDFSAEKQKQFGIEIMSRL
                     GFDFNRGRLDVSHHPFCGGISDDVRMTTRYSEQEFISSLMAITHETGHALYEQGLLKE
                     WSRQPVGHSLGMSVHESQSLLIEMQICRSLEFISFLVPIAKKYFKEAQNITEENLYFH
                     CTKVQPDLIRVEADEVTYPLHVILRYEIEQLLFSSEITIDDLPEVWDNFMRKYLGLST
                     KGNDKNGVMQDVHWPSGSFGYFPAYTFGALIAAQLFSAAKKAIPDINDQIKSGDFQSL
                     LIWLRENVHQKAKLFSYQELLCQATGEELNPQYYLAHIRQRYL"
     gene            1605..2108
                     /locus_tag="VAH76_002830"
     CDS             1605..2108
                     /locus_tag="VAH76_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1387413.1"
                     /translation="MLFFSSAMPKHKLTFTKLSNCMKYLDLAATWAEEEWGYIRNKGV
                     EFRKTILSDLKEHTYIGTFNGQPIAMFVLFDKDMSPEFNYGRLKPPVVSELMYVYIDE
                     PYRGLGFGKQIIDEAKQLASHSKSEYIMLDTLKPGLNRFYEKGGAKVIAENQLFLHPT
                     DVLTIKI"
     gene            2399..3913
                     /locus_tag="VAH76_002831"
     CDS             2399..3913
                     /locus_tag="VAH76_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEO1387414.1"
                     /translation="MYMDFGSDNMALSKEIQNKSLRSLIQKYARQLNETDKKLIELIN
                     NLAAADDIPLYAQNQNVINLLIKTIKDSQSEHSPDLEGTSYFIYGLITAEMEAQSEIN
                     RQRHLMQGRFDETALYLRTERDQMQDHDMTLLMPKSQGRIVVMAVLNRYDTHSANAII
                     EALASDVFNPEVHHIMIPVGPGHWRGVYLSKPQGGISDTVYDLELFDPYGPAGAAVLD
                     DYVLDLLNQCGVPKELVNIRHTGPKHPQGDAYSCGDFTCAYSHKKMKEFGAPEGSYNP
                     ILIDTLDNLGNEDNVLRMATREETRALVDKDRRRRHINPAIHSLQGADKEEPVSSIGK
                     PKAGAHGISFEIENANKSKKATIRQTVKESQLSPAMRTGLIIGGGTFTSGGVGAIIGG
                     LMGFFLLPGLGVLMGAIAGAFIGAGAGLLISGIIANAFWNSTPLNIIKTHESNTEIQG
                     IDLKFDSAGKPDKECSEKDEKPIHSPTLFFTPATQERAANDMVDELNSNGPKTG"
     gene            4105..>4774
                     /locus_tag="VAH76_002832"
     CDS             4105..>4774
                     /locus_tag="VAH76_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387415.1"
                     /translation="MTLQKYFPQFNEAWQSYSLCRSSLWWPLSYLLLTPLAVFIVFGL
                     IIPLGLNQLGWVSFDHALINYPYLLLVAGLLYLAFFPFLDGLYQMIKAHLDGKPINMN
                     TEDIDFINPGTSKVMGMSMGPILLTIIIMENVGSVFKPFQVLVLLAYALAIFTPILTV
                     NNPQSPLKKGFECIQFALANKKLVSKVLGLRLLILLSLFFPWIIIGITGEHVILKVIT
                     IMAGV"
BASE COUNT         1505 a          811 c          985 g         1473 t
ORIGIN      
        1 ctttgaaagc tgtcagatct gagtggaacc catattttta aagtataagc caagagtctt
       61 tcatgaatgc ctaccaacaa ttagaacaac attttaaaaa atattacgat tttgaaaatt
      121 tagcagccat tgtttcctgg gatgaagcaa cgatgatgcc agccgggggt gggcaggcgc
      181 gtgcagaagc gttggccaca ttaagtgctg tacaacatga atggttaacc aataagaaag
      241 taggtgactg gataaagcag gcaaaagatt tacccggttt atcattatgg cagcgaagaa
      301 atatctattg gattgataag caatatcagc aggcaacctg tattccagcg caactgatag
      361 aagagtttac gaatgaatct ttgatatgtg ttcaggcatg gcgggaatta cgagctaaaa
      421 atgattggcg ctcatttaaa ccgtacttta aaaagttatt tgaaaaagta aaaatgattg
      481 ccgatattaa atcacaaaga tttaataagc cttgtttgga tgtcttaatc gacgaatact
      541 ccccggattt ggattgcaag gccatcaccc cggtttttga tgtcttgaaa caaaaaatac
      601 cagctattat tcccaacgtt atcgaaaaac aaaaaaggcg agatattaaa gtgatttcag
      661 gtgatttttc tgcggaaaaa caaaagcaat ttgggattga aatcatgtct cgattggggt
      721 ttgactttaa tcgcggccga ttagatgtta gccaccatcc gttttgtggt ggtatttcag
      781 atgatgttcg aatgacaacc cgatatagcg agcaggagtt catatcttcc ttaatggcaa
      841 ttacccatga aacaggtcat gccttgtatg agcaaggctt gctaaaagaa tggagtcggc
      901 aacctgttgg ccattcatta ggaatgtctg tgcatgagag ccaatcattg cttattgaga
      961 tgcaaatttg tcgaagtttg gagtttataa gttttttggt tcctatcgcg aaaaaatatt
     1021 ttaaagaagc tcaaaatata actgaagaaa atctttattt tcattgtacg aaagtacaac
     1081 ccgatttaat tcgtgttgag gcagacgaag tcacttatcc actacatgta atactgcgtt
     1141 acgaaatcga acaacttttg ttttcttcag agattactat tgatgattta ccagaggtct
     1201 gggacaactt catgagaaaa tatttaggat tatcaacaaa agggaacgac aaaaatggtg
     1261 tgatgcaaga tgtgcattgg ccttctggaa gttttggcta ttttcctgcc tatacgtttg
     1321 gtgcattaat tgccgctcaa ttgttttctg cggccaaaaa agcaatccct gatatcaatg
     1381 atcaaatcaa atcaggtgac tttcaatcgt tgttaatttg gcttagagaa aatgttcatc
     1441 aaaaagcaaa gttattttct taccaagagt tattgtgtca agcaacaggc gaagagttaa
     1501 atccgcaata ttacttggct catatcagac agaggtattt ataaaacagg atggtcttga
     1561 ataaaatttg caggttgtgt cattttaatc aagagggtta tcagatgtta ttttttagtt
     1621 cagcaatgcc caaacataaa ctcactttta ctaaactttc taattgcatg aaatatttgg
     1681 atcttgccgc aacatgggca gaggaagaat ggggatatat tcgtaataaa ggggtagagt
     1741 ttcgtaaaac aatcctgtct gatttgaaag aacatactta tataggaact tttaatggac
     1801 aacccattgc catgttcgtt ttattcgaca aagacatgag tccggaattt aactatggaa
     1861 gattgaaacc cccggtagta agtgagttga tgtatgtcta tattgatgaa ccttacagag
     1921 gactgggttt tggaaagcaa attattgatg aagcgaaaca attagcaagc cattcaaaat
     1981 cggaatacat tatgttggat actttaaaac caggcctgaa tcgattttat gaaaaaggcg
     2041 gggcaaaagt gattgcagaa aatcaattgt ttttgcatcc aactgatgta ttgacaataa
     2101 agatataagc tgtgatctct atcacgggta agtaatgatc ttttttatgt agacatgagc
     2161 atgttatcgt gtttaagtaa tcatatattc aatgggtgca ataattaatc atgagagtaa
     2221 atttcttttc ggttaattat tttttatttc attagacctc ttgccctaaa acaaaagaga
     2281 caggttaggc aaaaggtcta atttagagcc aaaagcattg tcgaactgct tcagataggg
     2341 gcatacatta ttagagagga gggaagcttt attttttctt aattgtttta tatagcatct
     2401 gtatatggat tttgggagcg ataatatggc attaagtaaa gaaattcaga ataaaagttt
     2461 aagatcatta atccaaaaat atgccagaca acttaatgaa acagacaaga agctcataga
     2521 acttattaat aatctcgctg cagcagatga tattcctttg tatgcccaaa accaaaatgt
     2581 tatcaatttg ttaattaaaa ccattaaaga cagccagtct gaacacagtc cggatttgga
     2641 gggaacttcc tatttcattt atggattgat cactgcagaa atggaggctc aatctgaaat
     2701 aaaccgccaa agacatctta tgcagggacg ttttgatgaa acagcattgt atttaagaac
     2761 agagcgtgat caaatgcaag atcatgacat gactctactc atgccaaaat ctcaaggccg
     2821 tatcgttgtg atggcagttc tcaatcgtta tgacactcat tctgctaatg cgattataga
     2881 agctttggca agtgatgtat tcaatcccga agttcatcac attatgatac ctgttggacc
     2941 tggacattgg cgaggagttt atttaagcaa gccccaaggg ggcattagcg atacagttta
     3001 tgatcttgaa ttatttgatc cttatgggcc tgcaggcgct gcagtacttg atgactatgt
     3061 acttgattta ttaaaccagt gtggcgttcc caaagagctt gtgaatatac ggcatacggg
     3121 tccgaaacat ccgcaaggtg acgcttactc atgtggggat tttacttgtg cctatagcca
     3181 taagaaaatg aaagaatttg gagctccaga aggtagttat aatccaattc ttatcgatac
     3241 actggataat ttaggtaatg aagataatgt acttcgcatg gcaacgcgcg aagaaacgcg
     3301 agcgcttgtt gataaagaca gaagaagacg gcacattaat cctgctatcc attctctgca
     3361 gggggcagat aaagaagagc cagtatcgtc tattggcaaa cccaaagcag gtgcacacgg
     3421 gatatccttt gaaattgaaa atgctaataa atcaaaaaaa gcaactattc gccaaactgt
     3481 taaagaaagt caattatcac cggctatgcg aacaggtctt attataggtg gaggtacatt
     3541 cacctcagga ggagtagggg ctattattgg cggactcatg ggttttttcc ttttgccagg
     3601 actgggtgta ttaatggggg cgatagcggg agcttttatt ggtgcaggag caggtttatt
     3661 gatatcaggt attatagcca atgcattttg gaatagtact ccattaaata ttataaaaac
     3721 ccatgagagt aacaccgaga ttcaaggcat tgatttaaaa ttcgactctg cgggtaaacc
     3781 tgataaagaa tgttcggaaa aggacgagaa gcctatccac agtcctactt tatttttcac
     3841 tcccgctacg caagagagag ctgcaaatga catggtcgat gagttgaatt caaatggccc
     3901 taaaaccggg taaacatcag tgaaatattt cattcatctt tgcatcttgc aaataaacaa
     3961 tagcagtcga gctcattctg ttttacaaaa gcttcaggaa ccatgctgta aaaatttcaa
     4021 ttcagcattt gtagatattc ttttaattat gggattaatg tgctaatatg agtcatttaa
     4081 tataaatact gtccgaaaca tttcatgact ctgcaaaaat acttccctca gttcaatgag
     4141 gcatggcaat cttactcact ttgtcgctct tcgctgtggt ggcctttaag ctatcttcta
     4201 ttaacaccac ttgctgtatt tattgttttt ggattaatta taccattagg gttaaatcag
     4261 ttgggttggg ttagttttga ccatgcattg attaattatc cttatctttt gctagtagca
     4321 gggttacttt atttggcttt tttccccttt cttgatggac tataccagat gattaaagcc
     4381 catctggatg gcaagcctat caacatgaat actgaggata tcgattttat taaccctggt
     4441 acttccaaag taatgggcat gtctatgggc cccattttat taactattat tatcatggag
     4501 aacgttggtt ctgtctttaa accttttcaa gtattggtgc tgttagctta tgcgctagct
     4561 atttttacgc ctattttaac tgtcaacaac ccgcaatcac ctttgaaaaa gggattcgaa
     4621 tgcatacaat ttgctcttgc caataaaaaa ttggtaagta aggttttggg attacgtctt
     4681 ttgattcttc tatccttgtt ttttccctgg attattatag ggattactgg tgagcatgtc
     4741 attttaaagg ttattaccat catggcggga gtgc
//
LOCUS       DATNSN010000199         4773 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.200, whole genome shotgun sequence.
ACCESSION   DATNSN010000199 DATNSN010000000
VERSION     DATNSN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.200"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1267)
                     /locus_tag="VAH76_002833"
     CDS             complement(<1..1267)
                     /locus_tag="VAH76_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEO1387416.1"
                     /translation="MIILYDFLQESSVMPRSKNDSNLKKKSALQSKFKEQQWNHGSKE
                     HKSKFKFTQRKAKKKGPGMTHLPGNIYTLFTPVNGLKNNAQLTDTSKIMVNLHIDNMS
                     SSDYIPSAIDRTDLVIVQPVHLLRKTGGRGLFAREDIPKGTCIGIYTGEVYSEQEFEQ
                     YLMEHVGSDKSYAMYVGGRVVDAARKGNLTRYINFSDSQDNAEFVETTLNRKKVVKVI
                     TTKNIKAGQQLLINYNTYEEQASRYYYFLNPGDGWLSAQEFYQTYQSQYRLEQMPYNL
                     EGFDLKAGDRILMTQIGRIIFANYSLAKEQELNASDIDLPFLKVGSDEKILDFDEADT
                     FTPLMAACYLGQVENVKWLIEHGANIDQQQSHSGHCPLSLTLKGYSLAKDTKKYIDII
                     QLLIKNQVNLLVHDRSDKTFLHNAALILNN"
     gene            1411..2175
                     /gene="map"
                     /locus_tag="VAH76_002834"
     CDS             1411..2175
                     /gene="map"
                     /locus_tag="VAH76_002834"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEO1387417.1"
                     /translation="MGVTIKTPDEIEKMRVAGRLAAEVLEMIGPYVQVGITTDELNTI
                     CHDYIVNVQKAIPAPLNYNGFPKSICTSINHVVCHGIPGKKTLKDGDIINIDVTVIKN
                     EYHGDTSKMFIVGTPSVKAKHVVQIAHECLYIGIDMVKPGVQLGDIGHAIQQHAEKNR
                     CSVVRDYCGHGIGRVFHEDPQVLHYGVPGTGMKLEPGMTFTIEPMVNIGKHHTRLLPD
                     HWTVVTKDHSLSAQWEHTLLVTDTGVEILTLRNEER"
     gene            2162..4747
                     /gene="glnD"
                     /locus_tag="VAH76_002835"
     CDS             2162..4747
                     /gene="glnD"
                     /locus_tag="VAH76_002835"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEO1387418.1"
                     /translation="MKNDNRIIKNTIKQFKEKLCKDFSQKANITSITRKLAVFIDTIL
                     IQLFIKNKLHFGDNFCLLALGSYGRRELQLHSDIDLLILHTEKVSNIQLQRAQKFIQD
                     CWDVGLEVSHQITTVSSCANLASQDLSVISTIMDMFLLCGHGALMEELIYQTHTLHMW
                     PSHQYFFAKLQEQQNRYAKYGETAYNLEPNIKNGPGGLRDLQILLSISKRHFKIKKLA
                     EGIGYGFITDKEYEELKYCQNFLWRVRFALHMLAGKPEERLSFDYQVKLAQFFGYQDQ
                     SHILAIEQFMKDYFKVIKRNRELNEMLLQWFNETIVYHQKQKIIRLDDEFQLSNRFIE
                     VRNNRVFKQNPQSILKLFYWLVKRPDIEGVRASTIRLIRESLFLMGKRFRESKETANI
                     FINIFRTGNDPYDALQRMNRYGVLAHYLDCFATATGQMQYDLFHAYTVDQHTLFVIRN
                     ISRFKKNEYAKQFPLCAKIITALEKPEILYLGALFHDIAKGRGGDHSELGAIEAQQFT
                     QRHYMEAEDSKLIVWLVRYHLLMSQTAQRKDIYDPKTIEQFCQLLPHARYLDYLYLLT
                     VADICGTNPTLWNAWKDSLLKELYHAAKTRLHKQQELLDEAALISIRKQYAMDILISD
                     GISSRVIQDLWSQFKGKYFLHESPEVIARHTKAILNSKQFPVVIIMPHHSQGGTEVFI
                     YMPHKDERFTITTSVLSNHHVTIQEAAIITCDNQFDLDTYIILDENNQAFLNEQRARD
                     IQKNLCDHLANTGRLPAVSRRRLSRALTHFNVKTQINFIDDNTNHQTQLFLVTNDRPG
                     LLATISRVFLTLNIHLHNAKIATAGERVEDMFYISNQTGYSLNHEEKTILKEKLILEI
                     SKSKY"
BASE COUNT         1529 a          884 c          862 g         1498 t
ORIGIN      
        1 agttattcag gattaatgca gcattatgta aaaaagtctt gtctgatcgg tcatgcacta
       61 aaagattaac ctgattttta atcaatagtt gaatgatatc gatgtatttt ttggtatcct
      121 tcgctaaaga ataccctttc aatgtcaaac tcaaaggaca atgaccagaa tgactttgtt
      181 gttgatcgat attagcgcca tgttcaataa gccattttac attttcaact tgtccaagat
      241 aacaggcagc catcaaaggg gtgaatgtat ccgcctcatc aaaatctaat attttttcgt
      301 cagaacctac ttttaaaaat ggcaaatcga tatctgaggc atttaattct tgttcttttg
      361 caagggaata gttagcgaaa attatccgtc ctatttgagt cattaaaatt ctatctcctg
      421 ctttaagatc aaatccttcc aaattatagg gcatttgttc taatcggtac tgagattggt
      481 aagtttgata aaattcttgt gccgatagcc aaccatcgcc aggattaaga aagtagtaat
      541 acctggaagc ttgctcttcg taggtattat agttgattaa tagttgttgt cctgccttga
      601 tgtttttagt ggttataact tttactactt tcttgcgatt caatgtagtt tcaacaaact
      661 ctgcattatc ctggctatcc gaaaaattga tataacgagt caaattacct tttcttgctg
      721 catcaactac ccgtccccca acatacatcg cgtaactttt atcagaacca acatgctcca
      781 ttagatattg ttcaaattct tgttcagaat acacttctcc tgtgtaaata ccaatacaag
      841 tcccctttgg gatatcttct ctggcaaata aacctcgccc accggttttt ctgagtaaat
      901 ggacaggctg aacaataaca agatcagttc tgtctatagc agatggaatg taatcactgc
      961 ttgacatgtt atcaatatgc aaattaacca taattttgct cgtgtcagtc aattgagcat
     1021 tgtttttcaa tccattgaca ggggtaaaca gtgtgtaaat attacctggg aggtgtgtca
     1081 tcccaggtcc ttttttcttt gcctttcttt gggtaaattt aaatttagac ttgtgctctt
     1141 tgctaccatg attccattgc tgttctttaa actttgactg aagggcactt ttctttttaa
     1201 ggttgctatc attcttgctt cttggcataa cactgctctc ttgtaaaaaa tcatataaaa
     1261 taatcattaa ggagccatta tactctatta gagtattttt tgctctattt tgcatgtgat
     1321 tttcattgaa aatctattat tttgctgatt gaagagtatt gttgctcctc ttacccatgt
     1381 gctaacattt atgattttgg ataaaccata atgggcgtta caattaaaac tcctgatgaa
     1441 attgaaaaaa tgcgtgtcgc tggcagactg gcagccgaag tacttgagat gattgggcct
     1501 tatgttcaag ttggcattac aactgatgaa ttaaacacta tttgccacga ttatattgtt
     1561 aatgtacaaa aggctattcc cgccccttta aattataacg gtttccccaa atcaatttgc
     1621 acctcaatta atcatgtggt atgtcatgga ataccaggga aaaaaacttt aaaagatgga
     1681 gacatcatca atattgatgt cactgttata aaaaatgaat accatggtga taccagtaaa
     1741 atgtttattg taggcacccc ttctgtcaag gccaagcatg tagtgcagat agcacatgaa
     1801 tgcttgtata ttggaattga tatggttaaa cctggtgtcc agctaggcga tatagggcat
     1861 gccattcagc aacatgcaga aaaaaatcgt tgctctgtgg tgcgcgatta ctgcggacat
     1921 ggaattggcc gtgtatttca tgaggatcca caagtgcttc actatggagt tcccggaaca
     1981 gggatgaaat tagaaccagg gatgactttt actatagagc cgatggttaa tatagggaaa
     2041 catcatactc ggttattacc agatcattgg actgtcgtta ccaaagatca tagtctttca
     2101 gctcaatggg agcatacact tttggtaact gatacaggtg ttgaaattct gactttacgt
     2161 aatgaagaac gataatcgca tcataaaaaa tacgattaaa cagttcaagg agaagctgtg
     2221 caaggacttt agccaaaaag ctaatattac ctccatcaca cgcaagcttg ctgtatttat
     2281 tgatactatt ttaattcaat tatttattaa aaataagtta cactttggag ataatttttg
     2341 tctcttggct cttggaagct acgggcgtag agagcttcaa ctccactcag atattgactt
     2401 gttaatattg cacactgaaa aagtaagcaa tatccaatta caacgcgcac aaaaatttat
     2461 ccaggattgt tgggatgtgg ggctggaggt aagccatcaa attaccactg tttcttcatg
     2521 tgccaatttg gccagtcagg atttgagcgt aatttctacc attatggata tgttcttact
     2581 gtgtgggcat ggtgccttaa tggaggaatt aatttaccaa acacacacct tgcacatgtg
     2641 gcctagccat caatacttct ttgccaaact tcaggaacag caaaatcgtt atgcaaagta
     2701 tggagaaaca gcctacaacc ttgaacctaa tattaaaaat ggtccaggtg gattaagaga
     2761 cctgcaaatt ttgctaagca tcagcaaacg ccattttaaa attaaaaaat tagccgaggg
     2821 aatcggttac ggttttatta ctgataaaga gtatgaagaa ttaaaatact gccagaactt
     2881 tctttggcgc gttcgatttg ctttgcatat gttagcagga aagcctgagg agcgcttgtc
     2941 atttgattat caggttaaat tagctcaatt ttttggatat caagatcagt ctcatatctt
     3001 ggccatagag caatttatga aagactactt taaagtcatc aaacgcaata gagagctgaa
     3061 tgaaatgctc ttgcaatggt tcaatgaaac catagtctat catcaaaagc aaaaaattat
     3121 tcgtctggat gatgaatttc aactgtcaaa ccgattcatt gaagtacgca ataaccgtgt
     3181 atttaaacaa aatccgcaat ctatattgaa actattctat tggcttgtta aacgccctga
     3241 catagaagga gtaagggcaa gcacaatcag actaataaga gaatcattat ttttaatggg
     3301 caaacgcttt agagaatcaa aagaaaccgc taatatattt atcaacatat tcagaactgg
     3361 taatgatcca tacgatgcat tacaaagaat gaatagatat ggtgttctag cgcactatct
     3421 tgattgtttt gctacagcca ctggacaaat gcagtatgat ttattccatg cctatactgt
     3481 tgatcagcat acactatttg tgatccgtaa tatatctcgc tttaaaaaga acgaatatgc
     3541 caaacaattt cctttatgcg ccaaaatcat tactgcttta gagaaacccg aaattttgta
     3601 tctcggcgct ttatttcatg acattgccaa gggtcgggga ggagatcatt ctgaattagg
     3661 agcgattgag gcacaacaat ttacacaacg ccactatatg gaggcagaag atagcaaatt
     3721 gattgtttgg cttgttcgct accatttact catgtctcaa actgctcagc gtaaagatat
     3781 ttatgatcct aaaaccattg agcaattctg ccaacttctg cctcatgcaa ggtatttgga
     3841 ttatctttat ttactcacag tagcagatat ttgcggcaca aatcctactc tttggaatgc
     3901 ctggaaagat tcattactta aagagttata tcatgcagca aaaacaagac ttcataaaca
     3961 acaagagttg ctcgatgaag cagctctgat atccattcgt aaacaatacg ctatggatat
     4021 attaatttca gatgggatat cctccagagt catacaggat ttatggagtc aatttaaggg
     4081 aaaatatttt cttcatgaat ctcctgaggt gatagccaga cacaccaagg ccatactgaa
     4141 cagcaaacaa tttccagtag taataattat gccgcatcac agtcagggtg gaaccgaagt
     4201 atttatttac atgccacata aagatgagag atttactatc accacttcag tattaagcaa
     4261 tcatcacgtt actatacagg aagcggcaat tattacttgt gataatcaat ttgatctgga
     4321 tacttatatt attttagatg aaaacaatca ggcttttttg aatgaacaac gtgccagaga
     4381 catccaaaaa aatctgtgtg atcatctggc caatacagga cgcctccctg ctgtttctcg
     4441 cagaagatta tccagagcat tgactcattt taatgtcaaa acacaaatta actttattga
     4501 tgacaatacg aatcatcaaa cccaactatt tctcgttacc aatgacagac ctggactttt
     4561 ggcgactata agtcgtgtgt ttcttacact aaacattcat ttacataatg caaaaattgc
     4621 tacagctggt gagcgggtag aagatatgtt ttacatctcc aaccagacag gttattccct
     4681 gaatcatgag gaaaaaacaa ttttaaaaga aaaattaatt ttggaaattt ctaaaagcaa
     4741 atattaagaa atatgccaag ataaattact agc
//
LOCUS       DATNSN010000200         4704 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.201, whole genome shotgun sequence.
ACCESSION   DATNSN010000200 DATNSN010000000
VERSION     DATNSN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.201"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1420)
                     /locus_tag="VAH76_002836"
     CDS             complement(<1..1420)
                     /locus_tag="VAH76_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEO1387419.1"
                     /translation="MKRGLFVHLKHDLPASIVVFFVALPLCLGIALASHAPLFSGIIA
                     GVIGGVVVGFISGSALGVSGPAAGLVAIVITSVETLGSWEAFLLAGVIAGILQLIAGF
                     LRGGIIAYYFPSSVIKGMLTGIGIIIILKQIPHLLGHDVTPLLLSMSDGELDINDIRE
                     ALDDINPEVVIISIVSLLTLIFWEKVLVKKHKLFEMIQGPLVVVILGIALNLFYEHRL
                     TGFSLDPSELVQLPKFNKASELFNYLTLPDFSLITHLKIYKIAILIALIASLETLLCV
                     EAVDKLDPYKRVTSTDRELKAQGVGNILSCLIGGLPITQVIVRSSANVAFGAKSKLSA
                     ILHGLLLFICVISIPEILNEIPLASLASILIVIGYKLAKPALFKQMYKMGWEQFVPFM
                     ITIVGIIFSDLLFGITLGFSVAIFIILRHHFLNSHDIIKIRGKNKTQYCLKLAEEVTF
                     LNKGSIINELKNIPEGAEVIIDG"
     gene            complement(1672..2223)
                     /locus_tag="VAH76_002837"
     CDS             complement(1672..2223)
                     /locus_tag="VAH76_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEO1387420.1"
                     /translation="MDIKKLAPWNWFKEEETQNKSVPISTTGQNYVSPLSQLHQEIDR
                     IFDNVLRSVGISNLDIDKTLPHSTQNLLLKPCVDIAATDKEYTITVEVPGVEEDHIKL
                     ELTNDTLIIKGEKKHESEKKDKNIYRVERAYGSFQRVLSLPEDANQEDIKAQIKNGVL
                     TITMPRKEVSKPKGKLIDIKKAS"
     gene            complement(2375..2875)
                     /locus_tag="VAH76_002838"
     CDS             complement(2375..2875)
                     /locus_tag="VAH76_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEO1387421.1"
                     /translation="MNTTSLSLTPLLRHSVGFERFNDLFESMRNADDSTYAYPAYDIE
                     KHGEDSYMITMAVPGFQESDLNIMVQNDQLRVSGRIQEKETKESEYLHRGIVTRAFEQ
                     TFRLADHMKVTGAEIKNGLLSISLIREIPEEAKPRIIPIKSISDQESSKKGKTLEPES
                     KKKISN"
     gene            3412..>4704
                     /locus_tag="VAH76_002839"
     CDS             3412..>4704
                     /locus_tag="VAH76_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEO1387422.1"
                     /translation="MSKNLFYSVFLILLSMKAYSQDAVSRDAAMRAEPIEKKSIAYHN
                     VIDCDYKISPEISHIDSDIILNWAEYAAILSFNFNFASIEDQLNQLHACYTEKGWGQM
                     QNALHKSNNIETIKAEKLKVKGYKDGEAQIIEALDNQWKIILPLKVVYENDKKRVTHF
                     LNAYLTIGWKNKDSLGIMQMIAIPRLAPLAQKVTPADEVIKSMGIILAERKKASLDSA
                     RKITDSFFVPLFSNHGERSKRNLTLSNRAAQRVAIIQPMPNLNPTHNLVRMQSFDMPE
                     SAKIPYLALSKQRSLVNDSDYNKSTLSDFVSKTRKLYPNWLSQIGLHSSKLQFNSLIT
                     RINPLPLWRAFSQYQQSIRDKSDNIEAINKQKPILSAQIEGEPQFIEVKGNQWDIKLP
                     IKVVYQKDDEKITQRLDVNLALDQKMINNLMVKTKSVDS"
BASE COUNT         1497 a          876 c          859 g         1472 t
ORIGIN      
        1 acccatcaat aattacctct gcaccttctg gtatattctt taattcgttg attatacttc
       61 ctttatttaa aaaagttacc tcttccgcca gtttcagaca gtattgggtt ttgtttttgc
      121 cacggatttt aataatatca tgtgaattaa gaaagtgatg gcgcaagata ataaaaatag
      181 ctactgaaaa acctaaagta atcccgaaaa gcaaatcact aaaaataatt ccaacaattg
      241 taatcataaa tggtacaaac tgttcccacc ccattttata catttgtttg aataaagccg
      301 gcttggctaa cttatagcct ataacaatca aaatactggc taatgatgca agtggaattt
      361 cattgagtat ttctggtatg ctgatgacac aaataaaaag taataagcca tgtaaaatgg
      421 cggacagctt ggattttgct ccaaaagcga cattggcact actacgcaca ataacctgtg
      481 taatcggtag tccgccaatc aagcaagaga gtatatttcc tactccctgg gcttttaatt
      541 cacggtctgt agacgttact ctcttgtagg gatccagttt atcaacagct tctacgcaga
      601 gcagtgtttc caaactggcg attagggcaa tgaggattgc tattttgtat attttcaaat
      661 gagtaattag cgaaaaatct ggcagtgtta aataattaaa taattcggat gctttgttaa
      721 atttcggcag ttgaaccagt tccgatggat caagggaaaa acctgttagt cggtgttcat
      781 aaaacaagtt caaagcaatt cccaggatga ccacgacaag ggggccttgt atcatttcaa
      841 ataatttgtg tttttttacc agcactttct cccaaaaaat cagggttaaa agagaaacaa
      901 tgctgataat aacaacctca ggattaatat catccaatgc ttctctgatg tcattaatat
      961 ccaattcacc atccgacata ctcaatagaa gtggtgtgac gtcgtgccct aacaagtgag
     1021 gaatttgttt taaaataata atgataccga ttcccgtgag catgcctttg attaccgagg
     1081 agggaaaata gtaagcaata attccacctc gcagaaaacc tgcaattaat tgcaatattc
     1141 cagcaatcac cccagctaac aaaaaagctt cccagctgcc taatgtctct acagaggtaa
     1201 tgactattgc tacaagtccc gcagctggtc cactcactcc aagcgctgag ccgctgataa
     1261 accctacgac tactccacca ataacaccag caataattcc tgaaaaaaga ggagcatgtg
     1321 aagctaatgc aatgcccaga cataaaggta gagccacaaa aaaaacaaca atggatgctg
     1381 gtaagtcatg tttgagatgg acaaataaac cacgcttcat aaatggcctc gtttaagttg
     1441 tttgtttgag ctcctgtaat gctaatgtgc tttccaagtc taaagtatag tttatctgat
     1501 aattgttttg taaaacatca tattaattta ataacttgca atggcatacc tcaacctgtg
     1561 aagtcagagt ggcctggtac ccaattctaa ctttcccttt gcaattctct ttgctactga
     1621 agcaaagagg atgctcacgg ggcaaatcgc tcatagattt ctggcaataa atcaacttgc
     1681 ttttttaatg tctatcaatt tacctttggg ttttgatacc tccttacgcg gcattgtaat
     1741 agtgagtacc ccatttttaa tctgagcctt aatgtcttct tgattagcat cttcgggaag
     1801 tgataatacg cgttgaaatg agccataagc acgttcgact ctatagatgt ttttgtcttt
     1861 tttttctgac tcatgtttct tttcaccttt aatgatcaag gtgtcattgg ttaattccaa
     1921 tttaatatga tcttcttcta cgccaggcac ctcaactgtg atggtatatt ctttatccgt
     1981 tgctgcaata tcgacacagg gcttaagcaa cagattttgg gttgagtgag ggagtgtttt
     2041 gtctatatcc agattagaaa ttcccacgct tcgcaagaca ttatcaaata tcctatctat
     2101 ttcttggtgc agttggctca gaggagaaac atagttttgt ccagtggtac taattggtac
     2161 actcttattt tgagtttctt cctctttaaa ccaattccaa ggggcaagtt ttttaatgtc
     2221 catagtagta acctccatta aatttgggtt aagtcaatta atttattgtt gttattagat
     2281 ttatctggtt aacatcaaat caatcattcc caggagaaat cctgccattt tatttaaaat
     2341 ctgtgccgga ttttccatat ccccggcaca ttttttaatt ggaaattttt ttcttgcttt
     2401 ccggttcaag ggttttaccc tttttactgc tttcttgatc agaaatactc ttaattggaa
     2461 tgatgcgtgg tttcgcttct tccggtattt cacgaatcaa gctgatagac aatagcccat
     2521 tttttatttc ggcacctgtt actttcatgt gatctgcaag tcgaaaagtc tgttcaaagg
     2581 ctcgcgtcac aatgccacgg tgtaagtatt cagactcctt ggtttctttt tcctgaatgc
     2641 ggccgctaac tcttaactgg tcattttgca ccatgatgtt cagatcactc tcctgaaatc
     2701 ctggaacagc cattgtgatc atgtaactgt cttctccatg tttctcaatg tcataggccg
     2761 gataggcata ggttgaatca tcggcattgc gcatcgattc aaacaaatcg ttgaatcgtt
     2821 caaaaccaac ggaatgccgt agtaacgggg ttagtgataa tgaggtagtg ttcataatat
     2881 atctccttaa ctttgaggcc aagtttaata ttacccgagc actttactag ctgctccacg
     2941 tctcacaatt gaggtgtaaa actctattta gttgtaagct taaataaggt cgattgatac
     3001 aatttcaagt agcaaaataa tagattttta tcgtctaaaa ggaacacaca tgctgaatag
     3061 caattctttc catgggtcga aattagaata ctaaagtatt tgaaaaatta atgagttacc
     3121 accacactat taaacagtag aataacgtga gttatggata agaattatat aagaagcagt
     3181 cgatatccaa ggcccgtttg ggctgaggag atgcgtaagc atcgtctcga agccttggca
     3241 agaaacatga aattccatac aaggcttcga gacggcataa atgcctcctc agcctgaacg
     3301 atacaagata gtgaccaatt ttatccgtaa ctaacgttag aataaattaa attatccgat
     3361 aaatttgcac gttcgtaagg gatttgtaga tacagaataa ggaatttttt tatgtcaaaa
     3421 aatctattct atagtgtatt tcttatttta ttgagtatga aagcttactc tcaggacgct
     3481 gttagcagag atgccgcaat gcgagctgaa cccattgaaa aaaagagtat tgcttatcac
     3541 aatgttattg attgtgatta taaaatctca cctgaaattt ctcatattga ttcggatatc
     3601 attttaaatt gggctgaata tgcggctatt ctgtctttta atttcaattt tgcgtctata
     3661 gaggaccaat taaatcaatt gcacgcttgc tatactgaaa aggggtgggg gcaaatgcaa
     3721 aatgctttac ataaatcaaa taatatagaa accattaaag cagaaaaatt aaaagtaaag
     3781 ggctataaag atggggaggc tcaaataata gaggccttgg ataatcaatg gaaaattata
     3841 ttgcccttaa aagttgttta tgagaatgat aaaaaaagag tgacgcattt tcttaatgcc
     3901 tatctgacca taggctggaa aaataaagat agtttgggga ttatgcaaat gattgctatt
     3961 cctcgtctgg cgccactagc tcagaaggtt acgccggctg atgaggtaat taaaagtatg
     4021 ggcataatac tggctgagag aaaaaaggcc agtttagata gtgcaagaaa aattacagat
     4081 tctttttttg tgcctttatt ttcaaatcat ggagaacgtt ctaaaagaaa tcttactttg
     4141 agcaacaggg ctgcgcaaag agtggcaatt attcaaccaa tgccaaactt aaatcccact
     4201 cataatcttg tgcgaatgca atcatttgat atgcctgaat ctgcaaaaat tccatatctt
     4261 gctttatcca aacagagatc ccttgttaac gattctgatt ataacaagag cactctgagt
     4321 gattttgtca gcaaaactcg aaaactctat ccaaactggt taagccagat cggtttgcat
     4381 tcttctaaat tacaatttaa ttcattgatt actcgaatta atcccttgcc tttatggcgt
     4441 gctttttctc aatatcaaca atccataaga gataaatcag ataatattga ggcaatcaat
     4501 aagcaaaagc cgattttaag tgcacagata gaaggagagc cccagtttat agaagttaaa
     4561 ggaaatcaat gggatattaa attacctata aaagttgttt atcaaaaaga cgatgagaaa
     4621 ataactcaac gccttgatgt taatttagct ttagatcaaa aaatgataaa taatttgatg
     4681 gtaaagacta agagcgttga ttct
//
LOCUS       DATNSN010000201         4578 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.202, whole genome shotgun sequence.
ACCESSION   DATNSN010000201 DATNSN010000000
VERSION     DATNSN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.202"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(480..1310)
                     /locus_tag="VAH76_002840"
     CDS             complement(480..1310)
                     /locus_tag="VAH76_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387423.1"
                     /translation="MKLNLTVLSETHKNILRVQRKFVISALDAIAAYLVKNGDVGTEL
                     REMLNKEYMINYYDVIPDKGPLIAHEIELELLVTNENGEVEIKVFRKDFRKANEALIC
                     IVSSKLKGRKARLLDMLKDATGEQIKLINTHITQTENLIKECYYLKLRSQLLIAEVGG
                     LHFIDDILKQVSKNIKSDAHANYILFKKELQTLKELVQLNSPVREQRDYIFPYDTQDI
                     LAQRREFNKIFNNCKLLLSSLLQEGLLESTHENSIIMSFKHLQHLFFAKCDVNFIALR
                     "
     gene            1524..3287
                     /locus_tag="VAH76_002841"
     CDS             1524..3287
                     /locus_tag="VAH76_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387424.1"
                     /translation="MPNQYIQEIIANWVATLPGDLINNLGTLRSDNLDNNMKALEETL
                     NPILADAGYKAEVKALSLGFNKIIRLKIENTSEPVDLEEIKNEEIEIEEPFNYCLSIE
                     NLEMYIEPDVRTELANKENEAAAHLAPVYASNQTNAYRASLLPFYKDGTLDDKISFAT
                     YFDAASEESLDLDRYITLCSLKNIQNQYFKQHNNVAFTPEVMYAAGRYREFSYESINT
                     AMHLYELAEKKAEINKKLEGSNSPGKKIMSALRGRRSSEELNVEEEKKHINNFFDSLI
                     ESVQTALRDYPEKDAEIKDLINDLQAHLPNQESGNFLRNTRASLDYFNNAISKFIDNI
                     QFEADLGMVMENQQKVLLPVAIDVMRCADDFERNDIQYWDWKAPNIFMTEGQLVFTDT
                     KTLTQRQNDKVSFGLGHMTYRAPDSEMYQQTGESVDIEWAKGLAKFQIGLMLYEMTLG
                     WTGMTESLNTPHLLNIGIIKELDFDYPCFKTNTGQILEKQIKALTDLDPAKRPDFSKV
                     QEALITFTTENEMIRQHQEDISHLFEPSDLEMLSCPIQTNHQAPLTIKEKFKTVKVEE
                     ETITNEEKATPVEKSIIRPKQ"
     gene            3347..3994
                     /locus_tag="VAH76_002842"
     CDS             3347..3994
                     /locus_tag="VAH76_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1387425.1"
                     /translation="MNSKERKYWYKIGDSMDLGLIKSKIETLKEEFRNEAIWYILKGV
                     IQTNKSNVDQQCEVIRNLRDFLHGDLATFFNMTKVPGSDHGERLLQHAIIHGKDQLVL
                     TILEEPAVKVSFETSELRSTPLHLALMNGLSHGVIQLLTTNYSMTRKNNQNTTPEDIA
                     KITGNDEFLLLLAERQLAKSKGLNFFQLEKLSGTEESEDASDKYQFTQNKPGASS"
     gene            complement(4229..>4578)
                     /locus_tag="VAH76_002843"
     CDS             complement(4229..>4578)
                     /locus_tag="VAH76_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase substrate of
                     the Dot/Icm secretion system"
                     /protein_id="HEO1387426.1"
                     /translation="FLMEQNPIDINEYFTYIDENDFDIVMHCCNNKLFDKALVLLAFY
                     PDYFKRNYMSDNEGHNQFNINAFRKAIKDFNSNERNILLMHLGESGLHLPEALLAQLG
                     IMDSSITLESKFF"
BASE COUNT         1595 a          750 c          782 g         1451 t
ORIGIN      
        1 aatgagggcg gtacactttg tgccggtttg acttttgctt ggattaaagt aacgatcaag
       61 ggaaaatttg tgcgatcaca gggacaataa ttaaataatg ctccacgttt attagcaggt
      121 attgcggtgt ttttttgggg gcattccaca aggttcatat attatcaaat actctagaat
      181 acccataagt taagtcagtt actataagct tcaaaaagca gttattgtag tagtgtgatt
      241 acaaggggaa actgtcggta ttcgcactaa ttcaattaac ttatcgacaa tcgccttaat
      301 tcatatgggt gactggcaca tattagtgga tggctcgcac tgaaatttta gctctatatc
      361 gtttttgtaa tcagaatatt tttaacttct aaaattttta tggtttaaaa actcttgata
      421 ttagcttttc ttgctcgcac aggcatttct aatgtatgac caccacagaa tagcaatatt
      481 catcttaaag caataaaatt tacatcgcat tttgcaaaaa acagatgctg caaatgttta
      541 aaactcataa taattgaatt ctcatgagtg gattctaata atccttcctg caataaagaa
      601 gacaataaca atttgcaatt gttaaaaatt ttattaaatt ctcgtctttg agcaagaata
      661 tcttgagtat catagggaaa gatgtaatct ctctgctctc ttactggaga atttaattgg
      721 acaagttctt taagtgtctg cagttctttt ttaaatagaa tataattggc gtgtgcatcg
      781 ctttttatat ttttgctaac ttgtttaaga atgtcatcga taaaatgtaa accacctact
      841 tcagcgatga gtagctggct acggagtttt aaataataac attcttttat taggttttca
      901 gtttgagtta tatgagtatt aatgagcttt atttgttcac ctgtagcgtc tttaagcatg
      961 tctaacagtc ttgcttttct acctttcaat tttgagctaa ctatgcatat caatgcttca
     1021 ttagcttttc taaagtcttt tctaaatact tttatctcta cttcaccatt ttcattagtc
     1081 acaaggagtt ctaactcaat ttcatgtgct ataagaggtc ctttatcagg aattacatca
     1141 tagtagttaa tcatgtattc tttgttaagc atctcacgca attctgtacc cacgtcccca
     1201 ttctttacta aatatgcagc aattgcatca agcgcgctta ttacaaactt cctttgaact
     1261 cgcaaaatgt ttttgtgcgt ctctgataat accgtcagat ttagtttcat agtatcattt
     1321 aagaaaattg tattaattac aattatagac agtctttaat gaaatgatag aaaaccgcaa
     1381 tctgttgttg taactccatg aatctatccc atcactaact ccataaaatg ccacattcat
     1441 agagttatga caaaaatttc tatctaaaat tttaaatttc tgaatgtagt ctatactaaa
     1501 ataaaaacgg aatggataaa attatgccaa atcaatacat tcaagaaatt attgcgaact
     1561 gggttgccac ccttcctggt gatttaataa ataatttagg tactcttcgt tctgataatt
     1621 tagataataa catgaaagca ctagaggaaa cgcttaatcc aatattggcc gatgctggat
     1681 ataaagctga agtcaaggct ttatctctag ggtttaataa aataattcgt cttaaaatag
     1741 aaaacacctc tgagcctgtt gatttagaag agatcaaaaa tgaggaaata gaaatagaag
     1801 aacccttcaa ctattgtctc tcaatcgaaa atttggagat gtatatagaa ccagatgtca
     1861 gaacagaatt agccaataaa gagaatgagg cggctgcgca tcttgctcca gtttatgctt
     1921 ctaatcaaac aaatgcttat cgagcgtctc tcttgccatt ttataaagat gggacattag
     1981 atgataaaat aagttttgcg acatattttg atgccgcgtc agaggaatca ttggatctgg
     2041 ataggtatat aactttatgt tcattaaaaa acatacaaaa tcagtatttt aaacagcata
     2101 ataatgttgc tttcacacct gaagtcatgt acgcagcagg tagatataga gaatttagtt
     2161 acgaaagtat caatactgct atgcatctat atgaacttgc cgaaaaaaag gctgaaatca
     2221 acaagaagct tgaaggatcc aattctccag ggaaaaaaat aatgtccgct ttaaggggac
     2281 ggagatccag tgaagaactc aatgttgaag aagaaaaaaa acatatcaat aattttttcg
     2341 attctttaat agaatcagtt cagaccgcgt tgagagatta tccagaaaaa gatgcagaaa
     2401 taaaagattt aataaatgat ttgcaagctc atttaccaaa tcaagaaagc ggtaattttt
     2461 taagaaatac aagagcatct ctagattatt tcaataatgc tatttcaaaa tttatagata
     2521 atattcaatt tgaggctgat ctgggaatgg ttatggaaaa tcaacaaaaa gtactattgc
     2581 ctgtggcaat agatgttatg agatgcgctg atgactttga aagaaatgat atccagtatt
     2641 gggactggaa ggcgcctaat atctttatga ctgaagggca gttggttttt acagacacta
     2701 aaacattaac acaaagacag aatgataaag tttcctttgg tttaggacat atgacttata
     2761 gagctcctga cagtgagatg tatcaacaga caggtgagtc agtagatatt gaatgggcta
     2821 aaggacttgc taaatttcaa ataggtttga tgctttatga gatgacactt ggatggactg
     2881 gtatgactga atctttaaac actcctcatc tgcttaatat cggtataatt aaagagctgg
     2941 attttgatta tccttgcttt aaaaccaaca cgggtcagat tttggaaaaa cagattaaag
     3001 ccctaactga tctggatcct gctaaacgac cagattttag caaagtacaa gaagcattaa
     3061 taacctttac gacggaaaat gaaatgataa ggcagcatca agaagacatc tcacaccttt
     3121 tcgagccctc tgatcttgag atgctctcct gtccaataca aactaatcac caagctccac
     3181 tgactattaa agagaaattt aaaactgtta aagtggagga ggaaaccatt acgaatgaag
     3241 aaaaagcaac tccagttgaa aaatccataa taaggccaaa gcagtagaat atcctaaaat
     3301 taatctagag atatttctca tcataaaatt gaacggagta acttcaatga atagtaaaga
     3361 aagaaaatac tggtataaaa ttggtgatag catggacctt ggattaataa aatctaaaat
     3421 tgaaactttg aaagaagagt tcagaaatga agcgatttgg tatattctta agggtgttat
     3481 tcaaaccaat aaatccaatg ttgatcaaca atgtgaagtg attagaaatt tgcgagattt
     3541 tcttcatggt gatctggcaa ctttttttaa tatgacgaaa gttcctggga gcgatcatgg
     3601 cgaacgcttg cttcaacacg ccattattca cggaaaagat cagttggttc tgactatctt
     3661 ggaagaacca gcggtaaaag tatcttttga aacatccgaa ttacgctcca ctccattaca
     3721 tttagcatta atgaatggcc tttctcatgg ggtaattcag ttacttacaa caaactattc
     3781 tatgacacgt aagaataatc aaaatacaac tccagaagat attgcaaaga taacaggaaa
     3841 tgatgagttt ttattattac ttgctgaaag acaattagca aagagcaaag gattaaattt
     3901 cttccaatta gaaaaacttt caggaacaga agagagcgag gatgcttcag ataaatatca
     3961 atttacgcag aacaagccgg gagcatcaag ttaattttta tgcttcaaga aggagatgtt
     4021 atcgaatttt ttccggacta cccagtaaat tattctgtgt aactgtcaga tcaagtttga
     4081 actattacat ctcaggcaaa ttcgtctaac tcttctccgt tcattactgg ctatccttag
     4141 cctttcaggc tggagtgata gacagttaca caaatttatc tgcaatccca atttaagttt
     4201 cagatataaa atatgtcaat atgaaaaatt aaaagaactt gctttcaagt gtgatacttg
     4261 aatccataat gcctaattgc gctaacaagg cttcaggcaa atgcaaacca ctctccccta
     4321 ggtgcattaa taaaatatta cgctcatttg aattaaaatc ttttatcgct tttctaaaag
     4381 cattgatatt aaattgatta tgaccttcat tatcgctcat ataatttctt ttaaaataat
     4441 ccggataaaa agccaataaa acaagagctt tatcaaataa tttattgtta cagcaatgca
     4501 tgacgatatc gaaatcattc tcatcgatgt atgtaaagta ttcattgata tcaataggat
     4561 tttgttccat taaaaatt
//
LOCUS       DATNSN010000202         4517 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.203, whole genome shotgun sequence.
ACCESSION   DATNSN010000202 DATNSN010000000
VERSION     DATNSN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.203"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            205..1356
                     /locus_tag="VAH76_002844"
     CDS             205..1356
                     /locus_tag="VAH76_002844"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEO1387427.1"
                     /translation="MRLLSFKKSKIVSIGTELEFQIIDRNSLALVSRSKELMRALKDM
                     RYHDQIKPEITQSMIEINSSIHQSAKEMYDELLELQKILVETAASIDIAFCGGGTHPF
                     QQWTMQKIFPSKRFKKKFNQYRYLSKRATVFGQHIHIGCPTGDDAIYLTHALARYVPH
                     FIAISASSPFYLGINTNYCSSRSTIFNAFPLSGVIPYLRNWQEFSDYYRKMYRWKIIE
                     NMKDFYWDIRPKPELGTIEIRVCDTPLTLRKSILITAYIQALALYLLKEKPVQLSHDL
                     YYVYNYNRFQASRHGLEGELTVTDKDKPILIMDDILETIKKIEQYINGLGNSEYIEEL
                     YSDVINKQNDSVLINKIYKQDGSFTKLVAAQCELWLSDSKDRKWMTQPS"
     gene            1335..2366
                     /gene="legN"
                     /locus_tag="VAH76_002845"
     CDS             1335..2366
                     /gene="legN"
                     /locus_tag="VAH76_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegN"
                     /protein_id="HEO1387428.1"
                     /translation="MDDTTFLNTKEKILEIKPLMESAFGNILGYQELENMLAICTLVP
                     FSSNQILFNQGDRIKGIYIIIKGTVDLIARTLGKGITKIETCLAGNFLGETSFLLNEP
                     SATSAIAKDDVTCLFIPSAYFELMPAFSPEAKYKIMKAIGYQACGRLKQLHDKIIDFI
                     SHSDMQSLSFLGRVIQTFTQPKTHTLEESGVDKDQIYKKTLFHSFTQDETDELLEHAI
                     LLTANKNCVLIHENETIPVCYIVIYGAVQSNVIQNSKIAKLSVIGPDSLFASTACFLS
                     DTKFSITFITCESATLLKISETELQYFQKNKPMIWYKLFNLICGSIISLARSLKKLDV
                     RLNIEKYNQ"
     gene            2373..3290
                     /locus_tag="VAH76_002846"
     CDS             2373..3290
                     /locus_tag="VAH76_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEO1387429.1"
                     /translation="MCRILSYLGQPTVVEELLYKPDNSFIKQSYHQKYLPYLLNLAGF
                     GMAAWDRNSINPLQAYVYKTSKLPFYDENLHNLAEKIAPHCLLAHLCGVSYHDKQVVT
                     DQNVHPFLYPVTSIALAHNGMLHNFETMRYDLLKYVKTEYRKYIKGTTDSEWIYAVFL
                     SQLENPKSHFDASEIINAVIATLKILKTVRQQNNIVINSPVNLFLTNSDFIAATRFCL
                     DYGWKPKDAPQSTHFTYHSLWYTYGERYGFYENEYKMKGGTINSSIIIASEPLTENTT
                     TWLEVPEYTLIFANQIDGEIKITSQDINI"
     gene            complement(3418..4473)
                     /locus_tag="VAH76_002847"
     CDS             complement(3418..4473)
                     /locus_tag="VAH76_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEO1387430.1"
                     /translation="MVNSDLILSPYQFNEKITLKNRVIMAPMTRNMANDDFSPTRQMA
                     DYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQ
                     IWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
                     AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACIN
                     EIGGQSVGLRLSPGAYLNEIVGDERDAKVFQYVLEELNHYGIAYVHTGNFDDGIQFKE
                     LGFKTMTEFMRAHFKGTLIASGSYTLQQARKDIQNKKYDLIALGRPFIANPDLMNRIK
                     WNADFIPYENRMLDTLF"
BASE COUNT         1497 a          901 c          738 g         1381 t
ORIGIN      
        1 atgtaataac atggaagtat taaattcatg caatgaactt ccactttaat tgccctagaa
       61 cagtagcaaa caagctaaat tattgaacaa tttttatggg tttggggaaa tatgaagctt
      121 tatatctatt cccattagac aatcatctag ccaaaaaacc tgtctgctat acttaaacaa
      181 ggaaaataat agtcagagaa aggaatgaga ttattatctt ttaaaaaatc caaaattgtt
      241 tcaattggaa ctgaattgga gtttcagatt attgatcgca attcactggc tctggtttct
      301 cgttcaaaag aattgatgag agccttaaaa gatatgcgtt atcacgacca aattaagccg
      361 gaaattacgc aaagcatgat tgaaatcaat tcatccattc atcaatccgc taaagaaatg
      421 tatgacgaac tgttagagct gcaaaaaatt ttagtagaaa ccgctgcaag cattgatatt
      481 gctttttgcg gcggagggac acatccattc caacaatgga ccatgcaaaa aattttccct
      541 tcgaaacgat tcaaaaaaaa atttaaccaa tatcgttatc tcagcaagcg tgctactgtt
      601 tttggacaac atattcatat cggctgccca acaggagacg atgccattta tttaactcat
      661 gccctggcaa gatatgtgcc tcactttatt gcaatcagcg cttcatcacc tttctatctg
      721 ggtattaata caaattattg ctcatcacgc tcgactattt ttaatgcttt tccattaagt
      781 ggtgtcattc cctatctgag aaattggcaa gaattctctg attattatcg caaaatgtat
      841 cgttggaaaa tcattgaaaa tatgaaagac ttttattggg acattagacc aaaaccagaa
      901 cttggtacaa tagaaataag agtctgcgac acccccttaa ctcttagaaa atcgattctt
      961 attactgctt atattcaagc attggcactc tatctcctca aagagaaacc tgtccagctg
     1021 tctcatgatt tatactatgt ctataattac aacaggttcc aagccagcag acatggtctg
     1081 gaaggagaat taactgtcac tgataaagac aagcccatac ttatcatgga tgacatactt
     1141 gaaacaataa aaaaaattga acaatacatt aatggacttg gaaacagcga atatattgag
     1201 gaactgtata gtgacgtcat caataaacaa aatgatagtg ttctcataaa taaaatttat
     1261 aaacaagatg gctcatttac caagctggta gctgcacagt gcgaattatg gttaagtgat
     1321 tcaaaggata ggaaatggat gacacaacct tcttaaatac gaaagaaaaa atcctggaaa
     1381 tcaaaccatt aatggaaagt gctttcggca atattctagg atatcaagaa ctggagaata
     1441 tgttggcaat ctgtacgctt gtaccattct ccagcaatca aatcttgttt aatcaaggcg
     1501 atagaataaa aggtatttac atcattataa aaggaactgt tgatctgatt gccagaacct
     1561 taggcaaagg aataaccaag atagaaactt gccttgccgg taattttttg ggagaaactt
     1621 cctttctcct caatgagccc agtgccacat cagcgattgc taaggacgat gtgacatgct
     1681 tatttatccc ttctgcctat tttgaattga tgccagcatt ttcacctgaa gccaaataca
     1741 aaatcatgaa agcaattggg tatcaggctt gtggtcgttt aaaacaatta cacgataaaa
     1801 tcattgattt tatttctcat tctgatatgc aaagcttatc ttttttgggg cgcgtcattc
     1861 agactttcac acaacctaaa acacataccc tggaagagag tggagttgat aaagaccaaa
     1921 tttataaaaa aactttattt cactctttta ctcaagatga aaccgatgag ttgcttgaac
     1981 acgcgatact actaactgcc aataaaaatt gcgtattgat tcacgagaat gaaaccatac
     2041 cggtatgtta tatagtgatc tatggggcgg ttcaatccaa tgtcatacaa aacagtaaaa
     2101 ttgccaaatt gtctgttatt ggtcctgact cgttgtttgc cagtacagcc tgttttctga
     2161 gtgacactaa attttcaatt acatttatta cttgtgaatc agcaacatta ttaaaaatat
     2221 ctgaaaccga acttcaatat ttccaaaaaa ataaacccat gatctggtac aaattattta
     2281 atttgatatg tggttctatc atttcccttg ctcgttcctt aaaaaaattg gacgtgcgac
     2341 tgaacataga aaaatataat cagtgaggtg ctatgtgcag aatattgtct taccttgggc
     2401 aacccacagt ggttgaagag ttactttata agccggataa ctcctttatt aaacagagtt
     2461 atcatcaaaa atatttgcct tatttattaa atctggcggg ttttggtatg gctgcttggg
     2521 atagaaattc aattaatcct ttacaagctt atgtctataa aacatccaaa ctgccttttt
     2581 acgatgaaaa tttacataat ttagcagaaa aaatagcacc tcactgcctt ctcgcccatc
     2641 tatgtggggt ttcctatcat gataaacaag tagttactga tcaaaacgtg catccatttc
     2701 tctatccagt aaccagtatt gctttagcac ataatggtat gctccataac tttgaaacca
     2761 tgcgctatga tctgctgaaa tatgttaaaa ctgaatacag aaaatacatt aaaggaacta
     2821 cagacagtga gtggatctat gctgtttttc tttctcaatt agaaaatccc aagagtcatt
     2881 tcgacgcctc tgaaatcata aatgctgtaa ttgccacttt aaaaatttta aagacggtac
     2941 gccaacaaaa caacattgtc attaactcac cagttaatct ttttttgacc aatagtgatt
     3001 ttattgctgc gactcgattt tgtttggatt acggctggaa acccaaagac gctcctcaat
     3061 cgacacattt cacctatcat tcactatggt acacttatgg agagcgatac ggattttatg
     3121 aaaatgaata taaaatgaaa gggggaacca ttaattcaag cattattatt gcgtctgagc
     3181 cattaactga aaacacaacc acctggcttg aggtcccaga atatactttg atttttgcga
     3241 atcaaatcga tggtgaaatc aaaattactt cccaggatat taacatttga aatgttccat
     3301 atttagcatc ccaagacgct ttctaatcat ctccttgtac cctcttcatc acttcattcc
     3361 aaaattttct ggtaaaacag aaagcaagca ggataattaa ataccccgct caagtatcta
     3421 aaacaacgta tccaacattc tgttttcata aggtataaaa tccgcattcc acttgatacg
     3481 attcattaaa tcaggattag caataaatgg ccttcccaaa gcaattaaat cgtatttttt
     3541 attttgaata tcttttcttg cctgttgaag cgtgtagcta ccgctggcaa ttaaggttcc
     3601 tttaaaatga gctctcataa attctgtcat cgttttgaac cctaattctt tgaattgaat
     3661 gccatcatca aaattacctg tatgcacata agctattcca tagtgattca attcttccaa
     3721 tacatattgg aataccttgg catctcgctc atctccgaca atttcattaa gataagctcc
     3781 gggagaaagg cgcaatccta cagattgccc acctatttca ttgatacagg cttttacaac
     3841 ttccagcgca aatcgcccca tattctctgg atactgtcca tactgatcat gacgttgatt
     3901 ggtatggtaa tgtaaaaact gatcaatcaa ataaccattg gcaccatgaa tctcaatgcc
     3961 atcaaacccg gctatcatcg ctcttcttgc agccaacgca tagttttgga ttaactcatt
     4021 aatttcctct aatgtagccg cccttgattt tccataataa agatcatcgg cacgccggac
     4081 cttacctgtc atcatcgttt ctgaagcaga aatcggtaaa ttaccatgta aaaaaatcgg
     4141 atgagatacc ctgccaacat gccaaatttg caaaaatatt ttccctcccc gttcatgcac
     4201 agcatcagtt accattttcc aaccgtcaat atgggtttgc gtaaatattc ctggagcatt
     4261 actataacct tgaccatccg ggctaataat agtgccttct gtaataatca acccggcatc
     4321 agcccttcga gcgtaataat ctgccatctg cctggttgga ctgaaatcat cgttagccat
     4381 attacgagtc atgggagcca taatgactct gttctttagt gtaattttct cattaaactg
     4441 ataaggactt aaaattaaat cggagttaac catgataaat ctcctgcaga tcaaaacaat
     4501 atattggaat attgcaa
//
LOCUS       DATNSN010000203         4512 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.204, whole genome shotgun sequence.
ACCESSION   DATNSN010000203 DATNSN010000000
VERSION     DATNSN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.204"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            161..901
                     /gene="ravK"
                     /locus_tag="VAH76_002848"
     CDS             161..901
                     /gene="ravK"
                     /locus_tag="VAH76_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavK"
                     /protein_id="HEO1387431.1"
                     /translation="MVSLEHIQKLISECRKLGEDGLDNGTNGLIPELEIDVVPPSAFL
                     GVGNNPAIFVNSKTYKLMRTTHEKWVENKTIVFKSYLLSQPAIKIIGAIVHETGHAFN
                     VAAKIPNTEANACIFEIEVLMRLFQVKSPLLLGCTELDMQSYFKSRLADYNKCVKDCR
                     CLAEMVQLITHQFKLDEVSISEKENQIPLLSISNKWPGLFAKKQVAHDMEKLLTSPVT
                     ITPEVKILFYQLIKERFHSPETEIKLGI"
     gene            complement(1003..2466)
                     /locus_tag="VAH76_002849"
     CDS             complement(1003..2466)
                     /locus_tag="VAH76_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO1387432.1"
                     /translation="MKTNIPTLSKPIKDSGYRRGLKDRHVQLIALGGIIGSGYFLGTG
                     EVINLVGPSVFIAYLLGGLIIYLTMLCMGELAVAIPISGSFVTYTSDFISPTVACGVG
                     WSYWITWVAYIPAECVAGGIIMELFTGVSGYIWVVCFGLIITYINLAKVDTFGEIEFW
                     LALIKIISLLAFVFLAILIFFGLIHGSEPPGIIGFKYLLGDGGLLPNGAMSLLTAMVL
                     LLVNYQGSEIIGLAAGESENPARMIPHAIRNVTFRILFLYIIPVFCLVLIFPWQKAGL
                     SNSVFADALNFYGLKWAGAVTSFVTLSATLSCANSGFYGAVRSLNALARDGMAPHVLA
                     KFNQNSVPQNAVIATLIGVWILLGVGYFFGQTKLYIALLLVSGFTGTLAWLSLCVAQI
                     SFRNRLYKAGYSIKDLRYVTPYSPYTGILAVILMVGSLFFLLLNKDPIYKLSFIIGVV
                     SFIIPVIIYKVFDLSKVRKKALHVKSRVKFQDLFPPQ"
     gene            2845..3990
                     /locus_tag="VAH76_002850"
     CDS             2845..3990
                     /locus_tag="VAH76_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEO1387433.1"
                     /translation="MLRWIVLICVSLFLPLSAYADKSDCINHHCIAVIDAGSSSSRLH
                     IYAYDLDETNTPIAIREIWNRKAVPGLSTIEHENKSVSAYMEKLFSDAPAVLMPVYFY
                     STAGMRLLPKTHHRNVNQLVAEWFDRQYYWRLVTARTISGTEEGIFAWLATNYQLNLF
                     QQEPQTLAGVMDIGGGSVQIVIPVLNPSFVDHEHTTTIRLYGKEFTVFSHSFLGLGQN
                     EMGHQFFDLKFCFPEEYELPSGRQARGDAYACKDEIAILVNSVHSVSRKIKSVLAANP
                     IEKWYILGGITFMLNDKLFSLDNQYFTNRDLISFADQNICHQKWSTLEELYPDNLMVF
                     NYCMLPSYLYALLVHGYGLKSYQKVNFMPNSKNIDWTLGVVLQHKQS"
     gene            4007..4079
                     /locus_tag="VAH76_002851"
     tRNA            4007..4079
                     /locus_tag="VAH76_002851"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4040..4042,aa:Thr,seq:cgt)
BASE COUNT         1448 a          810 c          871 g         1383 t
ORIGIN      
        1 ttcaccttaa agagaaaagt atattcatat aattcataaa aaatcttgac tgaataattt
       61 caataccaaa taatggtctg atgtgaggca tattttgatc agtttgctat ctttgatttc
      121 tttatgatcg cagggatttc ttacaataag gaggggtatt atggtaagtt tggagcatat
      181 acaaaaactt atttcggaat gtaggaagtt gggtgaagat ggacttgata atggtactaa
      241 tgggttaatc cctgaattgg aaatcgatgt tgtccctccc agtgcgtttt taggggtagg
      301 taataatccg gcaatttttg taaattctaa aacatacaag ttaatgcgta caactcatga
      361 aaaatgggtt gaaaacaaaa caatagtttt taaaagttat ttgctttccc aaccggcgat
      421 caaaattatc ggggctattg ttcatgaaac tggtcatgct tttaatgtag ctgccaaaat
      481 tcccaatact gaagcaaatg cctgtatttt tgaaattgaa gtattaatga ggttgtttca
      541 agttaaaagt cccttgttgc taggatgtac ggaactggat atgcaatctt attttaaatc
      601 acggttagct gattacaaca aatgtgtgaa agactgccga tgtttagctg aaatggtcca
      661 attaataacc catcaattta agctggatga ggtgtcaatt tcagagaaag aaaatcaaat
      721 cccgcttctg tctatttcaa acaaatggcc aggtttattt gcaaaaaaac aggttgccca
      781 tgacatggag aaattattga cttctcctgt gactatcact ccagaggtta aaatcttgtt
      841 ttatcaattg attaaagagc gttttcattc tcctgaaaca gagataaagc ttggtatata
      901 gcaatatttt tgattgatgc aggtaattac tgtgatttta tgagtgaggc gtgcaaaatg
      961 aattctggca cgcccagaaa tacttcaaaa ataatgttta atttactgtg gcggaaataa
     1021 atcctgaaat ttcaccctgc ttttcacgtg caaagctttt ttcctaacct tggataaatc
     1081 aaagacctta tagatgatga cgggtataat gaagctcacc acaccaataa taaatgataa
     1141 tttatagatg ggatctttat ttagcagtaa gaaaaataag gagccaacca tcaaaataac
     1201 agcaagaatt ccagtataag gtgaataagg tgttacataa cggagatctt taatggagta
     1261 gccggcttta tacaaacggt ttctaaagct gatttgagca acgcataaag aaagccaagc
     1321 cagcgttccg gtaaaaccgg atactaataa taaagcaata tagagtttcg tttgaccaaa
     1381 aaagtaaccc acacccaaaa gaatccaaac tccaatcagt gtggcaataa cggcattctg
     1441 aggaacagag ttttgattaa atttggctaa aacatgtggg gccatcccat cacgtgccaa
     1501 agcatttaaa gaacgcacag caccataaaa tccggaatta gcgcaggata aggttgcaga
     1561 cagagtgaca aaactcgtta ctgctcctgc ccatttcagg ccgtagaaat taagcgcatc
     1621 agcgaagact gagttggata acccagcctt ttgccaggga aaaattaaaa ccaggcaaaa
     1681 tactggaatg atgtagagaa acaggattct gaaagtaacg ttacggatgg cgtgagggat
     1741 cattcgggca gggttttcag attctcccgc tgcaagtcct atgatttcag atccttggta
     1801 attaaccaac aaaagtacca tggctgtcaa caacgacatt gccccattag gtaacagacc
     1861 tccatcgcct aacaggtact taaatcctat tataccgggt ggttctgatc catgaatgag
     1921 gccaaagaaa attagtattg ccaggaaaac aaaggcgagt aatgagataa ttttaattaa
     1981 ggcgagccag aattcaattt caccaaacgt atcgacttta gcaaggttaa tataggtaat
     2041 aattaatccg aagcatacca cccatatgta accactcacc ccagtaaaca attccattat
     2101 gatccctccc gcaacacatt ctgcggggat ataggcgacc caggtaatcc aatatgacca
     2161 cccgacaccg caagcgacag ttggggatat aaaatcagaa gtgtaagtaa caaaagagcc
     2221 ggaaattgga atggcgactg ctaattcacc catacatagc atggttaggt aaataattaa
     2281 cccaccaagt aagtaggcaa taaacacaga ggggccaaca agattaatta cttcaccggt
     2341 tcctaaaaaa tatcctgagc cgataattcc acctagtgca atcagttgga catgacgatc
     2401 ttttaaacct cgtctgtaac cactgtcttt aatgggtttt gatagggttg gaatattagt
     2461 cttcacgggc tgttttttct cactatgtta gtaaaaaata atagagctaa tgttatattt
     2521 atccatataa atttaaaact gctccagatt aaaaaatatt tttcatctta ttggtaactc
     2581 ttatttttgc tatatggaag cagcagataa tgacgcccca aaatcaaaaa ttttgcgttt
     2641 atagcataaa ttttgaactt ttaaaagaaa caatttcatt aatttttatt ttttatcttt
     2701 cttcattttg attgaaaaat aagttcacat tgatatggat ttcctggtgt ctcactttta
     2761 ttcgcaaatt cagttcctgt tttgaccaaa aaaaacaatt aatgtaggat aaatgcgtta
     2821 tttgaggtga attaaggaaa gactatgttg cgatggatcg tgttaatttg tgtctctttg
     2881 tttctgcctt tatctgctta tgcggacaaa tccgattgta tcaatcatca ttgcattgcg
     2941 gtaattgatg ctggaagttc cagttcgcga ttacatattt atgcctatga tttggatgaa
     3001 acaaacacgc ccattgcaat tagagagatt tggaaccgaa aagccgttcc cggattgtct
     3061 acaattgagc atgaaaacaa atcggtttca gcctacatgg agaagctatt ttcagatgct
     3121 ccagctgttt taatgccagt ttatttttac tccacagcag gcatgagatt gttacctaaa
     3181 actcaccaca gaaatgtcaa tcaattggta gctgagtggt ttgacaggca atattactgg
     3241 cgacttgtga ctgcaagaac gatttctggt acagaggaag gtatttttgc ctggctcgca
     3301 accaattatc aactaaacct gtttcaacaa gagccccaaa ctttagctgg tgtgatggat
     3361 attggcggag gatctgttca gattgttatt cctgttttaa atccatcttt tgtggatcat
     3421 gagcatacaa ccacaattcg cctctacggc aaggaattta cggttttctc tcatagtttt
     3481 cttggtcttg gtcagaatga aatggggcat cagttttttg atttaaaatt ctgctttcca
     3541 gaggaatacg aactgccaag cgggcgccaa gctcgaggcg atgcctatgc ttgcaaagat
     3601 gaaattgcca ttttagtcaa tagcgttcat tctgtgagcc gaaagattaa atcggtatta
     3661 gccgctaatc ctattgaaaa atggtacatt ttgggaggca ttacctttat gctgaatgac
     3721 aaactgtttt ctctggataa tcaatatttt accaatcgtg atcttatttc ctttgcagat
     3781 caaaatattt gccatcaaaa atggtcaact cttgaagagc tctatcccga taatctcatg
     3841 gtgttcaact actgcatgct accttcctac ctttatgcct tgttagttca cggctatgga
     3901 cttaagagtt accagaaagt taatttcatg cccaatagca agaatatcga ttggaccttg
     3961 ggagtggtat tgcaacataa gcaatcttaa gtatactttt ctttaagccg ttgtagctca
     4021 gttggtagag caattgattc gtaatcaaga ggtcggaggt tcgactcctc tcagcggcat
     4081 agtaaaatca atagcttacg ttctttttgt ttttgtcatt tatttttggg tacactttgg
     4141 ggtacactta ttacttccca ctaggctgtg ggttcatggc tcttccccaa atccctgcca
     4201 cttcacgcat ctttctcttg ctcatccata cagcactttt aatttatttt gtgattggtg
     4261 aagactgatg caattttgat ttaaattaaa acaaaatatg agataaataa tcattctgtg
     4321 ggtttatata gatcatagtg tgaaaaaaca ctcctaaata tgattgtttt ggtgaaataa
     4381 tgaaattata atccgaactg ttattcaagg atattaataa ggagatgtta tgaaattata
     4441 ttattctaat agaatcgatg ccgatgaaga taaggatgaa tacatgcctt ggtaaataaa
     4501 ggagatatac gt
//
LOCUS       DATNSN010000204         4470 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.205, whole genome shotgun sequence.
ACCESSION   DATNSN010000204 DATNSN010000000
VERSION     DATNSN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.205"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(67..573)
                     /locus_tag="VAH76_002852"
     CDS             complement(67..573)
                     /locus_tag="VAH76_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO1387434.1"
                     /translation="MDTPSIYVACLASYNNAILHGVWIDATQSEDDIMKEIWEMLDNS
                     PETNAEEFAIHDYEGFGSMRIHEYEGISNIVEYASFIQEHGELGLALLCDYSVDDAQT
                     MLEDHYQGCYDSEVDFARQLFDDCYAHQMPDSLICYFDFETFAQDLFISDYCSVSFNG
                     FVYIFFNY"
     gene            complement(639..1061)
                     /locus_tag="VAH76_002853"
     CDS             complement(639..1061)
                     /locus_tag="VAH76_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO1387435.1"
                     /translation="MTAFLNRVQLIGNLGADPKSIAGKDGQSFVTTTLATNESFKQND
                     EWQTRVEWHQLVLFGKLTKVTEYLKKGSQVYLEGKLRSNQWTDNEGKARQALSIVVSH
                     IQLLNSPKSADESSNKTAEEHMAQMREMLQQDSEEIPF"
     gene            complement(1161..1355)
                     /locus_tag="VAH76_002854"
     CDS             complement(1161..1355)
                     /locus_tag="VAH76_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387436.1"
                     /translation="MQQQEPVQLEIEHLLFELTCCVNDLGRHRLQINTHDIQQAELSL
                     HKLESLISFVKQFDTQRIAC"
     gene            1872..2165
                     /locus_tag="VAH76_002855"
     CDS             1872..2165
                     /locus_tag="VAH76_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387437.1"
                     /translation="MTYLWTWSGRYFGYRQGNQLWTSSGIHAGMIYDDEVYDHKGHYL
                     GEILHGRLIRNPQKKYFRKYPSVCYGRKAAIAKYADYAGYAMYSGWEDFPAIH"
     gene            complement(2220..3119)
                     /locus_tag="VAH76_002856"
     CDS             complement(2220..3119)
                     /locus_tag="VAH76_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HEO1387438.1"
                     /translation="MSKSKLKNAQYSINECIKKIQNYSHASRADMKHMLNRCVKDLHE
                     LGYMVTHIKGLKPKHIYILVDYWKEQNKNPATIKNYMAKLRKVASVLNKPELVKEGND
                     NYKINERNYAPQYNKAINNVDFSKCSDPMIRLSLEAQSLFGLRREESMKIVLSDAWQG
                     NKLVIKPSWTKGGIGRDIKLTNEQQRQWLINAIKQVPAGQSLIPKEKTYKNHLAQYHE
                     VIAKMGLSKCHGLRHAYAQRRYIEITKSYDKTGKGLICPIQGGRIYKELNPLEKYWDR
                     TAREIISQSLGHSRLSVTKIYLG"
     gene            complement(3112..3918)
                     /locus_tag="VAH76_002857"
     CDS             complement(3112..3918)
                     /locus_tag="VAH76_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO1387439.1"
                     /translation="MRKYSLRQTANQYLKLGNQGSYKIRKQRAYVIRKMIDDLYTIGD
                     VPFSWKNIQSHHIHQLVAHWKNNKMRTSTIMNHMTIVRHYLTSIDCSIPNIDNKSLQL
                     SKTTRKRKRKLKIQHDHWQSWENPIPRLIMALQTEFGLTFQEAIYIKPEINIQADSIW
                     ITRNIAFNSLDRTIPIRFETQKAILNEIKMLTDGKSIAEFNDYEDTKLAWRKALKKHA
                     LPISKAYRYLYAKQMYQYLLPILGKYETYWVIRTEMGIKSRDSLWRYLNE"
BASE COUNT         1229 a          799 c          912 g         1530 t
ORIGIN      
        1 aaaaatagat attttgtata tatgcaatat tttcattttc attttagaaa cggccccata
       61 cggagcctaa taattgaaaa aaatatagac aaacccatta aaggatactg aacaataatc
      121 actgataaaa agatcttgag caaaagtctc aaaatcaaaa tagcagatca aactatccgg
      181 catttgatgg gcataacagt catcaaataa ttgcctggca aaatcaacct ctgaatcata
      241 acaaccttgg taatgatcct ccaacatggt ttgtgcatca tctaccgagt aatcacagag
      301 cagggctaat cctaactctc cgtgttcttg aatgaatgaa gcatattcca caatgttgct
      361 tattccttca tattcatgaa ttctcatgct gccgaaacct tcataatcgt ggatggcaaa
      421 ttcctcagca ttggtttctg ggctattatc caacatttcc cagatttctt tcatgatgtc
      481 atcttcgctt tgagtagcat ctatccatac accatgcagt atggcgttgt tgtaagaggc
      541 taaacaggcg acgtagattg aaggggtgtc catgggatta tctccttgta ttaagggagc
      601 tatcccacac gggagcttgc tcccgtgtta gggtatgatt aaaacggaat ttcttctgaa
      661 tcctgttgca acatttcacg catctgagcc atgtgctctt ctgcggtttt gttactggat
      721 tcgtcagctg attttggtga attgagaagc tgaatgtggc taacaacaat gcttaaggct
      781 tgacgcgctt tgccttcatt gtcagtccat tgattggagc gtaatttacc ttcgagatag
      841 acttgcgaac ctttctttaa atattcagtg actttagtca gctttccaaa aagcaccaac
      901 tgatgccatt ccactctggt ttgccattca tcattctgtt tgaatgactc attagtagcc
      961 aaagtggtgg tgacaaaaga ttgtccgtct tttccggcga tgcttttagg atcagcacct
     1021 aaattaccga ttaattgaac acggtttaaa aatgcagtca ttgtattctc ctgttgtttt
     1081 taatttgagc cgcataacag aaaaacatcc gcataggcag gtggttttct gttatgcgga
     1141 taagactttt attacttcaa ttaacaagcg attcgttgag tatcgaactg tttaacaaaa
     1201 gaaatcaggg attccaattt gtgtaaactt aactctgctt gctgtatgtc atgggtattg
     1261 atttgaagtc gatggcggcc taaatcattc acacaacaag ttagctcaaa gagcaaatgt
     1321 tcaatctcaa gttgtactgg ttcctgttgt tgcatggtag atccttgggg tagggaggat
     1381 tagccccaaa aaggactatt catcctaatt gggttatgca aaaaagatca ccgaaacatc
     1441 ggttaattta cctggttatt aatgatgatg tgcttaaagc actcctcggg cgtaaaaccg
     1501 taaagacatc tcctgatagg gtttccctgc taaacgcttt gccatggaat tagggaacaa
     1561 gatggcatat cacaagacct ggtggtttcg tcagaattgc actgactcat cagttgtgtt
     1621 gtgtttagat taacaaacta gaattggaat tcaataataa tttactgttg atattaaatg
     1681 aacgtaattg aaacaatatt taaattaaaa cacaatatat tgtgttttaa tttgtttgtt
     1741 attcaaaata atgggtttct ctgttgatta aacaaaatga tagtggcatc ataattatga
     1801 tcttaacttg atctcataga tcgttaagtg atctttgatt ttaattttag tccatttcaa
     1861 aggagttgaa tatgacatat ttatggacat ggagtggtag atattttggc tatcgacaag
     1921 gcaatcaatt atggacttca tctggtattc atgctggtat gatatatgat gatgaggtgt
     1981 atgatcataa agggcattat ctgggagaaa ttctacacgg acgtttaata aggaatccac
     2041 aaaaaaaata ttttcgtaaa tatccttcag tttgctatgg ccgaaaggcg gctatagcta
     2101 agtatgcgga ctatgcaggt tatgctatgt attctggatg ggaagacttc cctgcaatac
     2161 actagcgtgt atttggagca gcttattaaa ataagctgct tttttattgc tacatatggt
     2221 taaccaaggt agatcttcgt aacacttaat ctcgaatgcc caagtgattg gctaataatt
     2281 tcccttgcag tcctatccca atatttctca agaggattaa gctctttgta aattctacct
     2341 ccttgtattg ggcatataag gcctttacct gttttgtcat atgactttgt aatttcaata
     2401 tatcttctct gggcataagc atgacgaagt ccatggcatt tgcttaaccc catttttgct
     2461 atcacttcat gatactgagc tagatgattt ttataggtct tttctttggg aatgagggat
     2521 tgtccagcag gaacttgctt tatagcgttg ataagccatt gtcgttgttg ttcattagtg
     2581 agtttaatat ctcgtccaat tccacctttt gtccagctgg gctttataac caatttattt
     2641 ccttgccagg cgtcactgag tacaattttc atagattctt cacggcgtag accgaaaaga
     2701 gattgggctt ctagggataa acggatcatt gggtctgagc atttagaaaa atcgacatta
     2761 ttgattgctt tattatattg aggggcataa tttcgttcat tgattttata gttgtcgttg
     2821 ccttctttga ccagttctgg cttgtttagc actgaggcta cttttcgcag tttagccatg
     2881 taatttttaa ttgttgcggg atttttattc tgttctttcc agtaatcaac caggatatat
     2941 atgtgttttg gttttaatcc ttttatgtgt gttaccatat aacctagctc gtgtaggtct
     3001 ttgacacagc gattcaacat gtgtttcata tctgctcggc tggcgtggga gtagttttgg
     3061 attttcttaa tacattcatt gatggaatac tgtgcatttt taagtttgga cttactcatt
     3121 gagatacctc cataaagaat cacgggattt gatgcccatt tcggtgcgaa taacccaata
     3181 ggtttcatac ttgcctaata tgggcaaaag atactgatac atttgttttg catagaggta
     3241 ccggtatgct ttactaatcg gtaacgcatg cttttttaaa gcctttcgcc aggcaagttt
     3301 cgtgtcttca taatcattaa attcagcaat tgatttacca tcagtcagca ttttgatttc
     3361 attaaggatg gctttttgcg tttcaaatct gataggaatt gttctatcta aggaattaaa
     3421 cgcgatgttt ctggttatcc aaatgctgtc tgcttggatg ttaatttcag gttttatata
     3481 aatagcttct tgaaaggtca ggccaaattc tgtttgtaat gccatgataa ggcgcggtat
     3541 tggattttcc catgattgcc agtggtcgtg ttggattttg agttttcttt tacgttttct
     3601 agtggttttg ctaagttgta gggatttatt gtctatattg ggaatggagc agtctatgct
     3661 ggttagataa tgcctgacga tagtcatgtg attcataata gtgctggtac gcattttatt
     3721 gtttttccaa tgagctacca gttggtgaat atgatgtgat tggatgtttt tccacgaaaa
     3781 tggcacatca ccaatggtat agaggtcatc aatcatttta cgaatgacat atgctcgttg
     3841 ttttctgatt ttatagctgc cttgattgcc gagctttaag tattgattgg cagtttgtct
     3901 taagctgtat tttctcatca tcccatccga taaaaattta tttgccctgt ctttggttta
     3961 gtgttggtcg agcggctcac tcaaagttga aacctggcca aagccataaa gctcgatgta
     4021 gggggcaaat caatcgttat aaggcaaacg atctcgccga tttacaacag tttttgagtg
     4081 cttatttcct ccttgttttg atgattaata aaaacgacaa gacaatgact cttgtcatac
     4141 aagtccatgg tcaaattgaa ataaattgac ttaaactccc gagggagttg ccctgtcgga
     4201 tgacagagat tcctagatgg ttttccctac tgaacgtcca gccacggagt tagggaatga
     4261 agtggcttag cataagacgt cggttgacgt ttctccggaa tttcaccagc tcatcagtta
     4321 tgcttgattc caaattagca aagcggtgct tgcttcgcaa ggggttttgg aaagaatttt
     4381 gtccaatccc gattggcggg atcggacaaa attctgaaat gtgttgattt tacgcggtaa
     4441 aatcaaggtg atttttctgc gtcactgctg
//
LOCUS       DATNSN010000205         4458 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.206, whole genome shotgun sequence.
ACCESSION   DATNSN010000205 DATNSN010000000
VERSION     DATNSN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.206"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            117..2657
                     /gene="mutS"
                     /locus_tag="VAH76_002858"
     CDS             117..2657
                     /gene="mutS"
                     /locus_tag="VAH76_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO1387440.1"
                     /translation="MASSHTPMMQQYLRIKTDYPDMLLFYRMGDFYELFFDDAKRASQ
                     LLDLTLTHRGQSADKPIPMAGVPYHAVENYLARLLKKGESVAICEQIGDPATSKGPVE
                     RQVTRIITPGTVTDEALLDARKDNILLAIHTQKQKIGIAWVDLGGGRFHLQELTEEHQ
                     LNAELVRLQPAELLCKESTPLPSFCSNFAVKFRPGWEFDASNAHKLLCEQFSVTDLSA
                     FGEQNYPTALIAAGALLAYLKTTQKQSLPHLTTLTLEQSEDYLQLDASTQKHLELFEN
                     IHGGGEHCLLFILDKTACAMGSRLLKRWLGKPLKQHAIIQTRQQAIKEIIFLQQDVSL
                     HQLIKQCADVERIVSRIALKSARPRDLVSLLQTLTLLPAIHDELQENKSLLINEIKKE
                     ISPLTLLQQLLETAIIDNPPMLIRDGGVIAPGFDEELDELRNLSSNAHETLVKLEQEE
                     KNRTGLSTLKLGYNSVQGFYIELSKAQAQNAPPHFHRKQTLKNVERYITPELKLFEDK
                     VLSAQSKALAREKWLYDNLLEEIQQYIPELSDLAKSLAQLDVLVTLAERAQSLNWNCP
                     NLVPESGIMIQAGRHPVIEPLLQERFIANDLELKPNQNMLLITGPNMGGKSTYMRQTA
                     LIVLLAHIGSFVPADKVTLGPLDRIFTRIGASDDLSSGRSTFMVEMTETAQILRQATS
                     QSLVLIDEIGRGTSTYDGMALAYASCAFLASTIKAYTLFSTHYLELTELPKEFSCIRN
                     VHLQASIKTGQIVFLYRVEEGCANRSYGLEVAELAGIPKEVLKLAHEHLNQIQDTQSI
                     LVQTQIIKPPTSPVLTELKKIDPDRLTAKEALDLIYKLKHLECAESIN"
     gene            2670..4358
                     /locus_tag="VAH76_002859"
     CDS             2670..4358
                     /locus_tag="VAH76_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEO1387441.1"
                     /translation="MNRFAYLIILIILCFPLFAQDKKAGLFEIDGIKGKVLANVETRL
                     DELSQIKPLSQFTPTELQDQINKAIQPFGYFNAETSINNLNNKIIIKVQPGSQIRIAS
                     IKAMLTGEGAQNPLLRKTLKELPLHIGDPLFSEQYEKAKQNIINTAENMGYLHGVFKK
                     AEILIDEHKKSAQITLIFDTGPQYYFGQVQFDPTYISPQLLHRFVPFDPGQPYATDQV
                     LKLNDYLSNSGYFSSVLVKPQITDAQTVPVIVHLQPVPKYSYSFGLGYGTDTGVRGKA
                     ALHVIPVNRQGHKFNAVAQGSFRQNALQAQYVIPGKNPVTDQYALTGNFSNLNYNAGY
                     SNATLLSLSQLHNVNQFQRALSLNALYESFHYSLQPNTDQFLLYPKANITFSKTKNLL
                     FSPSGYNITFNALGANKAVLSHLNFGQLSLDAKAALTLDSLHLRLYGHTIQGITAIND
                     INELPLSLALLLGGTDNLKAYSFNSIGPGKIITYGGFEIQKEFKKNWYLVGFYDAGDV
                     YNPSVKNIQYDIGGGLMWVSPIGPIKVGLAQSVDNKMERIGHNPRLVISMGPDL"
BASE COUNT         1431 a          927 c          830 g         1270 t
ORIGIN      
        1 ttttttgatc acattgtctt tattgttttc ataaaaacca ataccaccaa catgccttac
       61 tgacttcaca caacactttt gttaaaattg gggcaatttc aatcggctca atcactatgg
      121 ctagctctca cacaccaatg atgcaacaat atttgcgcat taaaacagac tatcccgaca
      181 tgcttttatt ttatcgcatg ggtgattttt atgagttgtt ttttgatgat gccaaacgcg
      241 cgtcacaact tttggattta actctgacac atagaggaca atctgctgac aaacctattc
      301 ccatggccgg cgttccttat catgcggtag aaaactattt ggcacgatta ttaaaaaaag
      361 gggaatccgt agccatctgt gaacaaatcg gagatccagc taccagcaaa ggaccagtag
      421 aaagacaagt aacgcgaatt attactcctg gtacagtaac agatgaagca ttattagatg
      481 ccagaaaaga taatattttg ttagccatac acacccaaaa acaaaaaatt ggtattgcat
      541 gggttgattt aggtggcggt cgttttcatt tacaggagtt aacagaagag catcagttaa
      601 atgcagagtt agtgcgcctg caaccagcag agctcctctg caaagaatcc acgccgcttc
      661 cttcattttg ttctaacttt gctgttaaat tcagaccagg atgggaattt gacgcaagca
      721 acgcacataa attattgtgt gaacaattct ccgtgaccga tctttcggcc tttggcgaac
      781 aaaattaccc cactgccttg attgcagcag gcgctttatt agcttattta aaaaccactc
      841 aaaaacagtc attaccacat cttacaacac ttactcttga gcaatccgag gattacctgc
      901 aactggacgc atcgactcaa aagcacttgg aactttttga aaatatacat ggtggtggtg
      961 agcactgttt gcttttcata ttagataaaa cagcttgtgc gatgggaagc cgtttactca
     1021 aacgttggct gggaaaacca ttaaaacaac atgcaatcat tcaaacaaga caacaagcca
     1081 ttaaggaaat tatttttctt caacaagacg tttcacttca tcagctgatt aaacaatgcg
     1141 ccgatgtgga gcgcatagta tcacgtattg ctttaaaatc agcgcgccca cgtgatttgg
     1201 tttccttatt gcaaactctg acactgcttc cagcaatcca tgacgaatta caagaaaaca
     1261 agagcttgtt aatcaatgaa atcaagaaag aaataagccc tcttacactg ctgcagcaac
     1321 tgcttgaaac agccattata gacaacccgc caatgctaat tagggatggt ggagtcattg
     1381 ctccaggatt tgatgaagaa ctggatgagt tgcgtaattt aagcagcaat gctcatgaaa
     1441 cattagtaaa acttgaacag gaagaaaaaa atcgcacagg attatccact ctaaaactag
     1501 gttacaatag cgttcaggga ttttatattg aactgtcaaa ggcacaagct caaaatgcgc
     1561 cgccccattt tcacagaaag caaactttaa aaaatgtgga acgttatatt actcccgaac
     1621 taaagctgtt cgaagacaag gtgctttcag ctcaatccaa agcattggct cgcgaaaaat
     1681 ggctatatga caatttgctt gaagaaattc agcaatacat acctgagtta tcggatcttg
     1741 caaaatctct ggcgcaattg gacgtattgg tcactcttgc tgaaagagca caaagtttaa
     1801 attggaattg cccgaatctc gttcctgaat caggcattat gatacaagcc ggacgacatc
     1861 ctgtcattga gccattatta caagaacggt ttatcgctaa tgatttggaa cttaaaccaa
     1921 atcaaaatat gttactaatc actgggccca acatgggcgg caaatcgacc tacatgaggc
     1981 aaaccgcctt aatcgttctg ttggcacata ttggaagttt tgtacctgct gataaagtga
     2041 cattaggtcc attagaccga atctttactc gcattggggc cagtgatgat ttatcctctg
     2101 gacgttccac ttttatggtg gaaatgactg aaacagctca aatattaagg caggcaacca
     2161 gccaaagcct cgtattaatc gatgaaattg gtcgtggtac cagtacctat gatggaatgg
     2221 cattagctta cgcaagctgt gcctttcttg cgtcaacaat aaaagcctat actttgtttt
     2281 ctactcatta cctggagttg accgaattac ccaaagaatt ctcttgtatt cgcaacgtgc
     2341 atttgcaagc atccatcaag acaggacaaa ttgttttctt gtaccgtgtc gaagaaggtt
     2401 gcgcaaatcg cagttacgga ttagaagtcg cagaattagc tggcatacct aaagaagtgc
     2461 taaagctggc tcatgaacat ctgaatcaga tacaagatac tcaatctatc ctggtccaga
     2521 ctcaaattat aaagcctccc acatctccag ttttaactga actaaaaaaa attgatccag
     2581 atagattaac tgccaaagaa gcgttggatt taatttataa gttaaaacac ctggagtgtg
     2641 ccgagagcat caattgatgt agttattaca tgaataggtt tgcatattta atcatactaa
     2701 tcattttgtg ttttccattg tttgctcagg acaaaaaggc cgggctgttt gagattgatg
     2761 gtataaaagg aaaagttctt gctaatgtgg aaactcgttt agatgaattg agccaaatta
     2821 aacctttaag ccaatttacc ccaactgaac ttcaagacca aattaataaa gccattcaac
     2881 catttggata tttcaatgcg gaaaccagta ttaataatct caacaataaa ataataataa
     2941 aagttcagcc tgggtctcaa attcgtattg cctccattaa agcgatgcta acaggagagg
     3001 gagcacaaaa tccattatta cgaaaaactc tgaaagagtt acctctgcat attggagatc
     3061 ctctgttttc agaacaatac gaaaaagcaa aacaaaatat tataaatacc gcagaaaata
     3121 tggggtattt acacggcgta tttaaaaaag ctgaaatatt gatagatgaa cacaaaaaat
     3181 cggctcaaat caccctgatt tttgatacgg ggccccaata ctattttggc caggtgcaat
     3241 ttgacccaac ctatatttct cctcagctat tacatcgttt cgtaccattt gatcctggcc
     3301 aaccttatgc cacagatcaa gttttgaaat tgaatgatta tttatcgaac agcggttact
     3361 ttagttctgt actggtaaaa ccccaaatta ctgacgccca aacagtaccg gtcattgttc
     3421 atttgcaacc tgtacctaaa tattcctatt catttggcct tggctatgga acagacacag
     3481 gagtgcgtgg aaaagcagct cttcatgtga ttccagtcaa tcgccaagga cataagttta
     3541 atgccgtagc gcaaggctct ttcaggcaaa acgccttaca agcccaatat gtaatccccg
     3601 gaaaaaaccc tgttaccgat caatatgcct tgaccggtaa tttttcaaat ttaaactata
     3661 atgcaggtta tagtaatgct actctcttat ccctgagcca attgcataat gtgaatcaat
     3721 ttcaaagagc gctttcactg aatgctctgt atgaaagttt tcattactct ctacaaccca
     3781 atacggatca gtttttgctc taccccaaag ccaatataac tttcagtaaa actaaaaatc
     3841 tcttgttttc gccatcaggg tataatatta cttttaacgc gcttggggcg aataaggctg
     3901 tcctctccca tctgaacttt ggacaacttt cactggatgc caaagccgcg ctcacattgg
     3961 attcactgca tttgcgcctg tatgggcata ccatccaagg tattacagca atcaatgata
     4021 tcaatgaatt accgctttcc ctggcattac ttctgggagg aactgacaac cttaaagcct
     4081 atagttttaa ctctatcgga ccaggaaaaa ttatcactta tggtggcttt gaaattcaaa
     4141 aagaattcaa aaaaaactgg tatcttgttg gattttacga tgctggagat gtttacaatc
     4201 catccgttaa aaacattcaa tacgatattg gaggcggtct gatgtgggtt tcccctattg
     4261 ggccaattaa agttggatta gcccaatcag tcgataataa gatggagaga ataggacaca
     4321 atccaagatt ggttatcagt atgggaccag acttatgatg atattaaaat ttatcaaaaa
     4381 aggattttac tactgcttaa taacagcaat atgtctggtt agtttggtta tctttttgat
     4441 ttcaactacc ccggggct
//
LOCUS       DATNSN010000206         4415 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.207, whole genome shotgun sequence.
ACCESSION   DATNSN010000206 DATNSN010000000
VERSION     DATNSN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.207"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..323)
                     /gene="fliO"
                     /locus_tag="VAH76_002860"
     CDS             complement(<1..323)
                     /gene="fliO"
                     /locus_tag="VAH76_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEO1387442.1"
                     /translation="MKSKVLHLAFLSLSLISSVGWSSTLSPNTISYGELIRIISGLLF
                     VLFIIIFLSWIVKRMHRVNFTSSKGFQTVASMILGPKEKVVLLKIGDRYLLIGVGSYS
                     INLIC"
     gene            complement(320..649)
                     /gene="fliN"
                     /locus_tag="VAH76_002861"
     CDS             complement(320..649)
                     /gene="fliN"
                     /locus_tag="VAH76_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEO1387443.1"
                     /translation="MTQVNETSTEQLNQGNPLNPDPGNEKLELILDIPVSVTVEIGRT
                     KMTIRNLLQLNQGGIVALDRLAGDPLDVLVNGTLVAHGEVVVVNDKFGVRLTDIVSKA
                     ERIKRLK"
     gene            complement(659..1720)
                     /gene="fliM"
                     /locus_tag="VAH76_002862"
     CDS             complement(659..1720)
                     /gene="fliM"
                     /locus_tag="VAH76_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEO1387444.1"
                     /translation="MTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPARKKPE
                     DTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYH
                     LTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
                     YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHAWEPIIQLDVTKFNDETNPQ
                     LVNIAEPEEMLLVARFVLNFGKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNW
                     INSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTA
                     TLGSANEKRALKVIKIIRY"
     gene            complement(2165..2404)
                     /locus_tag="VAH76_002863"
     CDS             complement(2165..2404)
                     /locus_tag="VAH76_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387445.1"
                     /translation="MNKIFNLASIACFLFFSLIAFAADIPGLDEGTDKLNMEECVGEN
                     YQQCLDDVCLTSDEIDCNSNCQSLAEDKCEQQAVD"
     gene            2523..3818
                     /locus_tag="VAH76_002864"
     CDS             2523..3818
                     /locus_tag="VAH76_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO1387446.1"
                     /translation="MRSKRILLTNFTRAIYSESCCIRPDTEKELIHYIAQNQPKNMLT
                     RGAGLSYSDSCLNQDGLVIDTSRFNHFIDFDVETGIVVCQGGTPFKDLFLLHDEFIPP
                     VLPGTAFATVAGGIAHDVHGKNNHSAGSFGHHISWFDLLIGDQIMHCSREKNSDLFFA
                     TIAGLGLTGIITQVAIRLKKAPRHLAIKNKPFASIQELIAEMATYGLQQDYQVAWLDL
                     LYSEPRAILSTANYCESPNYPDKKLKIYSLPKLPFGLIKKWNMKVFNQYFYSRQKENE
                     MMSLTQYNNPLDKISHWNRLYGPRGLIQFQAVFDQDKAAQIINHILEIIRTHSATPTL
                     TVLKLFTQPGLGLLSFCRPGFTLAIDFINNPHAKKAISAMNQFIAENDGCVYLAKDLL
                     LNPEQYFTMYENQSKFAQILTKYRCPMRSDLATRLGIIK"
     gene            3815..>4415
                     /locus_tag="VAH76_002865"
     CDS             3815..>4415
                     /locus_tag="VAH76_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO1387447.1"
                     /translation="MMQRKWVVLGATSVIAEQFAHFAAQAGNHLCLVARNSEQLALIS
                     KDIQLRYKIPCNTITINLSEPNEQLLTILETRERELDLFIAHCDFTDNNHLNPQSIKH
                     LIDTNISSTAILINTYLQSQQEKHRIVYLSSVASCRGRAKNSLYGASKAAIEIFLEGL
                     QQKATPHQHILIARLGYIDTKQTYGLPGVFYAAQPHDCAK"
BASE COUNT         1420 a          899 c          723 g         1373 t
ORIGIN      
        1 tcacatatta gattaatgga atatgaccct acacctatta ataaatatct gtccccaatt
       61 ttcagcaata caaccttttc ctttggacct aaaatcatac ttgcaacagt ttggaatcct
      121 ttagatgaag taaagtttac tctatgcata cgcttcacta tccaagataa aaaaataata
      181 atgaataata caaataagag tcccgaaatg attcttatca gttcgccata gctgatcgtg
      241 tttgggctta aagtagatga ccaaccgacc gaactgatta gcgaaagact caaaaatgca
      301 agatggagta ctttgctttt catttgagac gttttattcg ttcagccttg ctaacgatat
      361 cagttaatct gacaccaaat ttatcgttaa ctacaacgac ttcaccatgt gcgaccaaag
      421 taccattgac tagcacatca aggggatcac cagccagcct atcaagcgct acgattcctc
      481 cctgatttaa ttgcagtaaa ttacgaatag tcattttggt acggccgatt tcgacagtca
      541 ctgagacagg gatatctaaa attaattcca atttctcatt gccaggatct ggattcagtg
      601 gattcccttg atttaattgc tctgtgctgg tttcgttcac ttgagtcatc cttatcccct
      661 aataacgtat tattttaata actttcaaag ctcgcttttc attggcactg cctaaggttg
      721 cagtaaagct tggtgtccct tctatatcca gagttacctc ctcgtttatt tccaaaggga
      781 caaaatctcc cactttccaa gccattacct gacccagagt actgactgtt tccgccatag
      841 aagcacttac cgtcaattca acgtccatga gttcttcttt cagagaatta atccagttag
      901 gatcaatttc atcatcaggt cttgatgccc ctagctccaa ttgctgtttg ataggttcaa
      961 gcatggagta aggcaaaata aagtaaaaag ccccggtttc cttaccaaaa ttcaagacga
     1021 accgcgccac aagcaacatt tcttctggct cggcaatatt aactaattgg ggatttgttt
     1081 cgtcattgaa ttttgttaca tccagttgaa ttattggttc ccaggcatga attaaattag
     1141 ttaccagttt tttagttacg acctccataa cacgaagttc agtagcagta aaatctgttt
     1201 tatccttttg agcaccaaac tggctatttc cgccaaaata ataatcgacc aagtcataca
     1261 caaaggtact gtcaaacaaa ataattcctt taccacgaag aggcttgaat ttatagatac
     1321 ctatcaggct aggattaggt aaactcctca taaactcctt atgctttgta ataagtagcg
     1381 gatcttgttt aatttcaaaa tctctagccg ttaaatgata aatatctgct gcaaacagac
     1441 gaactgccct gtcataaatt ttgtctaata ccggaagctg tcctttaaca atacgctctt
     1501 gtccggtaaa attaagtgtt ttgactcctt cttccgctac ttttttgtca gaatcaaaat
     1561 tagagtttaa ttcctggctg gtagtatctt ctggtttttt acgagctggc tgatctcttg
     1621 aactcaattc ttcatcattc gtttcatcat caattgactc atccactgaa tccagtaatg
     1681 catcgatctc ttcttgtgat aacacatctt tctcagtcat aagcaatcca ttcgcatctc
     1741 aaccagattt tcaaagttat tttaaaattg aagtgttgat tagtttaaaa ttagctcaat
     1801 caatcagtaa caactataga atggttatgt ttatgcaaag cccgtgccat ccaagaaata
     1861 tatcaataaa tcaaatagat agtgtaataa tcaaatcttg attagctcat ttaattagac
     1921 tccgccaatg ttttgacgta aaaatcataa ggtttatatt gataggactt acgaaaaact
     1981 ccaaagtcga ggcagaaaat gttttggcga gaggatttag ataaactaaa tgactgaatt
     2041 aaaaatattt tttaacaaag agattggggt ttttcataag tcttatgcac agtatttgag
     2101 gttgtgagcg ttccttaatg gtagtgaaat cacttcaaaa ataaatgttt agaaaatcct
     2161 ccacttagtc tactgcttgt tgctcgcatt tatcttcagc taaactttga caattggagt
     2221 tacaatctat ttcatcagag gttaaacaaa catcatccag gcattgctga taattttccc
     2281 caacacattc ttccatattt aatttatcag tgccctcatc cagaccagga atatccgcgg
     2341 caaatgctat caaggaaaaa aataaaaagc aggctatgct ggcaagattg aatattttgt
     2401 tcattagaat accttccatt gatatttgat ttaattatag acatcctata gcctttgttc
     2461 ctgtttccat aataaaaaaa tcatgcgaca ctcactcaac tgtctattta aaaatttctg
     2521 atatgcgcag caaaagaatc ttattaacta attttacccg cgcaatttat agtgaatcct
     2581 gttgtataag gccagacaca gaaaaagaac ttattcacta tattgcacaa aatcagccta
     2641 aaaacatgct aaccaggggt gcaggattga gttacagtga tagctgtcta aatcaagacg
     2701 gcctggtaat tgatactagt cgctttaatc atttcatcga ttttgatgtc gaaacaggca
     2761 tcgtagtatg tcagggtgga acaccattta aggatctgtt tttacttcat gatgaattca
     2821 ttcccccagt tctcccaggc acagcatttg ccactgtagc aggtggaata gcccatgatg
     2881 ttcatggtaa aaataaccat tcagcgggaa gttttggaca tcatatttct tggtttgatt
     2941 tattgatagg cgatcaaatc atgcattgta gccgcgaaaa aaatagtgat ctatttttcg
     3001 caactatagc aggtttaggt ttaacaggca ttattacaca agtcgctatt cgccttaaaa
     3061 aagcaccacg ccatctggca ataaaaaata aaccatttgc atctatccaa gaattaattg
     3121 cagaaatggc gacttatggt cttcagcaag attaccaagt agcctggtta gacttgttat
     3181 actcagaacc cagagccatt ttatcaacag caaattattg tgaatcgcca aactatcctg
     3241 ataaaaaatt aaaaatttat agcctgccta aattaccatt tggacttatc aaaaaatgga
     3301 acatgaaagt atttaatcaa tatttttact ccagacaaaa agaaaatgaa atgatgagtc
     3361 tcacccaata caataatcct ctggataaaa tatcacattg gaaccgactc tacggaccta
     3421 gaggacttat acagtttcaa gccgtatttg atcaagataa ggcagcgcaa attattaatc
     3481 acatattaga gattattagg actcatagtg ctactccaac cctcactgtc cttaaactat
     3541 ttacacagcc tgggttaggt ttgctttcgt tttgtcggcc tggatttact ctggctattg
     3601 attttattaa taatcctcac gccaaaaaag caataagcgc gatgaatcaa ttcattgcag
     3661 aaaacgatgg gtgtgtttat ttggctaagg atttattact aaatccagaa caatatttta
     3721 caatgtacga aaatcaatcg aaatttgctc aaatacttac aaaatatcga tgccccatgc
     3781 gttcagatct tgcgacacgt ttaggaatca taaaatgatg cagaggaaat gggttgtttt
     3841 aggagctact tcagttattg ccgaacaatt cgcacatttt gctgcgcaag ccggtaacca
     3901 tttatgttta gttgcacgca actccgagca attagcctta atctcaaaag atattcaatt
     3961 gcgatataaa atcccttgta acacgataac tatcaattta tccgagccta acgagcaatt
     4021 gcttaccatt ttagaaacca gagaaagaga attggattta tttattgcgc attgtgattt
     4081 taccgataat aaccatttaa atccgcaatc gattaaacat ttaattgata ccaatatctc
     4141 atctacagcg atcctgatta atacctattt acaatcccaa caagaaaaac atcgcatcgt
     4201 ttatttaagc tctgttgctt cctgccgcgg ccgtgccaaa aatagtcttt atggtgccag
     4261 taaagcagca attgaaatat ttctcgaagg cctgcagcaa aaagcaactc cacatcaaca
     4321 tatcttaatc gctcgccttg gatacattga tacaaaacaa acgtatggac tacccggagt
     4381 tttttacgca gctcaacctc atgactgtgc aaagg
//
LOCUS       DATNSN010000207         4358 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.208, whole genome shotgun sequence.
ACCESSION   DATNSN010000207 DATNSN010000000
VERSION     DATNSN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.208"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            27..1769
                     /gene="lbtA"
                     /locus_tag="VAH76_002866"
     CDS             27..1769
                     /gene="lbtA"
                     /locus_tag="VAH76_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin biosynthesis protein
                     LbtA"
                     /protein_id="HEO1387448.1"
                     /translation="MAAVLNNSYKINNSLHSLLLERDLLVSQSEMEHFISIAHQQCLD
                     RLQHAALREGLIDKEITSLHIHDYLDLLYHQQKKQKPEKTFDRWPLLKKELDESINNQ
                     ALALAYYHCWQQSLKQQAKNHSSLWSWLSEQHNKQQILTFLEQWGCIGHPYHPNFRAK
                     MGFIPEEVIRYSPEFNAQVDISWLAIHRSVAFTSIDATSYSLLFAQHFAKEYQLWNYS
                     VQKQQLDPEQYLPLPVHPWQWNNKLQTLASVLINSKQLLITEAFQKTKPSMSFRTMMP
                     LDNKGPHLKLATAIHTTSALRTVSPASVSNSSPLSSWLRQLLTQYQSFEKPLFLARDL
                     AGINTTHPSISPQNKKHLAMIVRENPLELIDASQTLVPVAALYALSPVSQRALFTEII
                     EASGMDPELYFIEYSRCILECQLHLLLSYGVALEAQQQNTLVIFQSHRPVGLVIRDLG
                     GIRVCFNSLYESTPKPNLHPDSTISCHELSDLFNTFIYGNLLSNLTFCINCLHDNYNL
                     TKKNLWILLKQIANQLLQNFKKKINPLIYQKFQQHLFSLPWQHKSLLSMRLNQNQDNL
                     VFFSLDNPLSQDNE"
     gene            1762..2976
                     /gene="lbtB"
                     /locus_tag="VAH76_002867"
     CDS             1762..2976
                     /gene="lbtB"
                     /locus_tag="VAH76_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin export MFS transporter LbtB"
                     /protein_id="HEO1387449.1"
                     /translation="MNKPVQKMMLSGQFLMILALEMTNPFLPLLIAHQGNTPVPSVVI
                     YNTLSLILPMLANILLTPIWGLAADRYGYKPMLMRASWALVLTQASMIFVNSVFWILI
                     IRLLQGAFAGFLVAMQTYALSITEWQNKSTQLSRLQSSKAIATSTAGFLGGLALSFTN
                     YQGLFGLATLICLGTTVAMHYKLPSPPKHQIKKKSQTLNHSYTSKSVFFFLCVLIMLT
                     QIAKFLPDPGFTLYLNKYCSNNLVLIGFLYSLPAMGMLCSSVWCGKQFDYCRSQPSLV
                     NQYLIRYSVFGAILMVIQANVDNFYMFGLIRILWGVVLAALLPALFALCSDRNLLPGY
                     ALGLANSFAKLGNLIGLLLGGLFAFYLPYPAIFLIIAGIYSLFAIFVYGYERLNVRQM
                     TDFSNSYFNVKS"
     gene            2973..4133
                     /gene="lbtC"
                     /locus_tag="VAH76_002868"
     CDS             2973..4133
                     /gene="lbtC"
                     /locus_tag="VAH76_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038838172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin import MFS transporter LbtC"
                     /protein_id="HEO1387450.1"
                     /translation="MKKNALLIVLLTWFLGNMDIHFLTPALPSLVDYFSVTPNTAQLT
                     ISLFLLGKALSMILWGLLSERYGRKPIFIGGLLLFILSNFLIALNHSILLFLVCRFLQ
                     GISVGATLLMGRAMINDTRNEQNATKYFAWLFTLAGLFICFLPFFGGLINSYWNWQIA
                     SLIIAAYGLLLLPLCRGLKETKPHYVKFPRLGHSIMVVFNHPLFVSYLLISALMMAGE
                     SAFNTSASFILIKGENFTSAQYGTIKTTMAIMHLLGTACCGLIAKYYHSIQLTKLGVR
                     FFIFAAFLMWLFVFSAENIYLTFIVPMVIYYFGTGFIVASATASAVRPFPQQMALALA
                     ITLFCQFNCSAFFSFITSMIGIQQVETFMFLLSLVSILSFLALLKLQRMEPC"
BASE COUNT         1270 a          875 c          745 g         1468 t
ORIGIN      
        1 tttcatactt attcacggaa ataatcatgg cagcagtttt aaataattct tataaaataa
       61 ataactcatt acacagttta ttacttgaaa gagatcttct tgtttcacaa tcagaaatgg
      121 aacatttcat atccattgcg caccagcaat gtttagatcg attgcaacat gcagcacttc
      181 gtgaaggatt aatagacaag gaaataacca gtcttcatat acatgattat cttgatttac
      241 tttatcatca acagaaaaaa caaaaaccgg aaaaaacatt tgatcgatgg cctcttctaa
      301 aaaaggaact cgatgaaagc atcaacaatc aggcgttagc cctcgcttat tatcattgct
      361 ggcaacaatc gctgaaacaa caggcgaaaa accactccag tttatggtca tggttatcag
      421 agcaacataa taaacaacaa attctgactt ttttagagca atggggttgc atagggcacc
      481 cgtatcatcc taatttccgc gcaaaaatgg ggtttattcc tgaagaagtg attcgatact
      541 cccctgaatt taacgcgcag gtagatattt cctggctggc aattcatcgc tccgtagcgt
      601 ttacttcaat tgatgcgact agctattctt tgttgtttgc ccaacatttt gctaaagaat
      661 atcaattgtg gaattattct gtccaaaaac aacaattaga tcccgaacag tatttgcctc
      721 ttccagttca tccctggcaa tggaataaca aattacaaac tctggcctct gtattaatca
      781 attcaaagca attattaata acagaggctt ttcaaaaaac caaaccatcc atgtcgttcc
      841 gaacgatgat gcctttagat aacaaaggtc cacacttaaa attagctaca gcaatccata
      901 ctacttctgc tttacggact gtatctcctg catcagtaag caattcgtct cctctttcca
      961 gttggcttag gcaattactg actcagtatc aaagttttga aaagccacta tttctagcca
     1021 gggatttagc ggggattaac accacacacc catccatttc cccacaaaat aaaaagcatt
     1081 tggctatgat agtacgtgaa aatccactgg aattgatcga tgcgtcacaa acattagtac
     1141 ctgtagctgc actctatgct ctttctccag tcagtcaacg tgctttattc actgaaatta
     1201 ttgaagcaag tggcatggat cctgaattat attttattga atacagccga tgtattctcg
     1261 aatgccagct tcatttatta ttaagctatg gtgttgcgtt ggaagcccaa caacaaaaca
     1321 ctttagtcat ttttcagtct catcggcctg ttggattagt tattcgagat cttggcggca
     1381 tcagggtctg ttttaattcg ctatatgagt caacacccaa acctaatctt catccagact
     1441 ctaccatttc atgccacgaa ttgagtgatc tctttaatac cttcatttat ggaaatttac
     1501 tgagtaactt aactttttgc attaattgtt tacatgataa ctataacttg actaaaaaaa
     1561 acctttggat cctgctaaaa caaattgcaa atcaattatt acaaaatttc aaaaagaaaa
     1621 ttaatccatt gatataccaa aaattccaac aacatttatt ctctcttccc tggcaacata
     1681 aaagtttgct gagcatgaga ctcaatcaaa atcaggataa cctggtattt tttagtcttg
     1741 acaacccatt gagccaagat aatgaataaa ccagttcaaa aaatgatgct ctcaggtcag
     1801 tttttgatga tattggcctt ggaaatgacg aatccttttc taccactatt gatcgcgcac
     1861 caaggcaaca caccagtacc atcggtagtg atttataaca cgctgagtct aattctgccg
     1921 atgctggcta acattctatt aacaccaatc tggggcttgg cagctgatcg atatggctat
     1981 aaacccatgt taatgagagc atcctgggcc ttggtgttga cccaggccag tatgattttt
     2041 gtaaattctg tattctggat tttaatcatc cgattactgc aaggagcttt tgcaggtttt
     2101 cttgttgcaa tgcaaaccta tgccctaagt attactgaat ggcaaaacaa aagcacacaa
     2161 ttatctcgct tgcaatcctc caaggcaatt gccacatcta cagccgggtt tctcggagga
     2221 ttagctttaa gcttcactaa ctatcaagga ttatttggtt tggcaacatt gatatgcctg
     2281 ggcactactg tagcgatgca ttacaaatta ccctcgccac caaagcacca gataaagaaa
     2341 aaatcacaaa cattaaatca ttcttacaca tcaaaaagtg ttttcttctt tctttgcgta
     2401 ttaatcatgc taacccaaat tgccaagttt ttacctgacc cagggtttac cctgtatcta
     2461 aataagtatt gttcaaataa tctggtacta attggatttc tttattcttt accagcaatg
     2521 ggaatgctat gtagttcagt atggtgtggc aaacagtttg actactgtcg aagtcaacct
     2581 tctttggtta accaatattt aatacgctat agtgtattcg gagccatcct catggtcatt
     2641 caagccaatg tagataattt ctatatgttt ggccttatac gcattttgtg gggagtcgta
     2701 cttgcggctt tattgcccgc attatttgct ttgtgcagtg atcgtaattt gttaccaggt
     2761 tatgccttgg gcttagccaa ttcatttgca aaactgggga atctaattgg attgttattg
     2821 ggtggtcttt ttgcatttta tctaccgtat cctgcaattt ttctgataat tgccggaatt
     2881 tatagtttat tcgcaatttt cgtttacggg tatgaacggc taaatgtcag gcagatgaca
     2941 gatttttcta attcttattt taacgtgaaa tcatgaaaaa aaatgctctt cttatagtgt
     3001 tattaacctg gtttcttgga aacatggata tacatttcct tactccagcc ttgccatcat
     3061 tagttgatta cttctcggta actcccaata cagcccaact aactatcagt ctttttcttt
     3121 tggggaaagc cctcagtatg attttatggg gtcttctttc cgaacgttat gggagaaaac
     3181 caatcttcat tggaggactg ttgttattta ttctgagcaa ttttctgatt gctctaaacc
     3241 actccatcct tctcttttta gtatgtcgat ttctccaggg aattagcgta ggcgctaccc
     3301 ttctaatggg tagagcaatg attaatgata cccgtaatga acaaaatgcc acaaaatact
     3361 ttgcgtggtt atttactctg gctgggctgt ttatttgctt tcttccattt tttggaggtt
     3421 tgataaacag ttattggaat tggcaaatcg cctctcttat cattgctgcc tatggcctgc
     3481 ttttattacc tttatgtcga ggtttaaaag aaaccaaacc ccattatgta aaattccctc
     3541 ggctaggtca tagtattatg gtggtcttca accatcccct ttttgtttct tacctcctta
     3601 tttctgcctt gatgatggct ggagaatcag cgtttaatac cagcgcctct tttattctta
     3661 ttaaaggaga aaattttact agcgctcagt atggtacaat caagaccact atggcaatta
     3721 tgcatttatt aggtaccgct tgttgtggct taatagcaaa atattatcat agtatccaat
     3781 taacaaaatt aggagtacgt ttttttattt tcgcagcatt cttgatgtgg ttatttgtct
     3841 tttctgctga aaatatatat ttgactttta tagtgcctat ggtgatttat tattttggta
     3901 caggtttcat tgttgcatca gcaacagcat cagctgttcg cccttttcct cagcaaatgg
     3961 cccttgcatt agctatcact ttattttgtc aatttaactg ctccgctttc tttagtttta
     4021 ttaccagcat gataggaatt cagcaagtag aaacttttat gttcttatta agtttggtaa
     4081 gtattttgag ttttcttgcc cttctaaaac ttcaacgaat ggagccctgc tgaaaaaacc
     4141 atgatttttg atcctggtaa gaaaatataa agtttgcgtg tatttctgcc taaagaggac
     4201 tatgcttatt taaaataagt tcttaatcgt atttcgctta gaacctgtgt acttccttca
     4261 attagaagga aaaaatatgg atagtaattt taaaatcagt atacgtgtca gcatcattac
     4321 cttatttgtg cttttgctaa gcctggtagg gttcacta
//
LOCUS       DATNSN010000208         4350 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.209, whole genome shotgun sequence.
ACCESSION   DATNSN010000208 DATNSN010000000
VERSION     DATNSN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.209"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            172..248
                     /locus_tag="VAH76_002869"
     tRNA            172..248
                     /locus_tag="VAH76_002869"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Pro,seq:cgg)
     gene            357..1079
                     /locus_tag="VAH76_002870"
     CDS             357..1079
                     /locus_tag="VAH76_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEO1387451.1"
                     /translation="MCKILKSIYISLMLIVSPQIKAASDTCHPKINPRLPQYIAGYGS
                     LIDEQSKKTTDSKAYENIPVLIKGFKRSWCAHGNMPGFNATFLSLNENKKGTFNGVIY
                     RVSDPKEIELYDTRETVYCRKELSAEQIKIYTKKIPEKIQVWIYYPKSTQNDQPSREF
                     PITQSYVDIFIRGCIKIEEKFNIKDFAKECILTTDNWPEQHWVNDRVYPRRPSAYEPY
                     ARKIDALLKEHLPKQFKNITIE"
     gene            complement(1418..1762)
                     /locus_tag="VAH76_002871"
     CDS             complement(1418..1762)
                     /locus_tag="VAH76_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEO1387452.1"
                     /translation="MVVYEVNLSIDNEIYRDYKDWLDEHILEMLKFPGFLNATVMHQS
                     IDGDSSDNQKHLTVQYQLESAEDLQNYFEEHAPKMRGDGMNRFKGRFTATRRTFEVEA
                     VITAVNEYVNRV"
     gene            complement(1765..2967)
                     /locus_tag="VAH76_002872"
     CDS             complement(1765..2967)
                     /locus_tag="VAH76_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387453.1"
                     /translation="MTNLVRISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQSSAI
                     NFTILAYVVSYVVKPFSGMVFGYLSDSYGRKQVLSVTTLLMTVSTAVIGLLPTSLPGI
                     DLWIALFSCRIIQGLSISGEFSNALIFAVEQGKDRPAFSGSMAFMGGILGLLLANLSV
                     FILLNLLPYEQMIQYGWRIPFLISAIIWLALYQIRRFINEPFAQKMNDTKHFSSLVKK
                     YRKELVICFVAASLSASAFYMTFVYMPTLLSSVIQNQTHHKSVWVTLIALSVYFICLP
                     LFGSLADRVGITKQITAASALYLLFSYVCFDYISHFNYVVIFCSLILLALIQSLYNSA
                     LPAFMVSLFPAIHRGKALAISYNTSLSIFGGLMPYVILSHKSFMNPGIVISICAILTL
                     VVLRFVRE"
     gene            3041..3529
                     /locus_tag="VAH76_002873"
     CDS             3041..3529
                     /locus_tag="VAH76_002873"
                     /inference="COORDINATES: protein motif:HMM:NF015437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEO1387454.1"
                     /translation="MIHDIPKRIFLPFDEKQELLFNKQLRPLNALGCDYFYYLVSDQT
                     SKSKSYRFCTHEDWMAFYYEEKLIENDPLKRIVEKSNISVLPWNQASFMNKHEKQTMS
                     GRSAFGLYNGVSIVSKFNNKNYIFVLATEHRDHDLARYLLLEKNYELKKLMHGCIASF
                     EG"
     gene            complement(3542..>4350)
                     /locus_tag="VAH76_002874"
     CDS             complement(3542..>4350)
                     /locus_tag="VAH76_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEO1387455.1"
                     /translation="AGQVSVANLVAMSRSAKNLVIMGDQMQLGQPSQGTHPGESGLSI
                     LDYLLHETPTIADDMGVFLGTTYRMHSTVNHFISKFIYDSNLEPHPDNDVRVVKVPDG
                     YQGKLNKEAGIIFVPVEHEGNTYASDEEVSEINLLAQELMGRTFIEKLGETRKISWND
                     ILFVAPYNYQVNKLKQALDEQARVGSVDKFQGQEAPIVFLSMCTSDANESPRGIDFLF
                     DKHRINVAISRAQSLAIVVGNPELCAVTVNRVEQLKLVNLFNALVNQSES"
BASE COUNT         1559 a          816 c          701 g         1274 t
ORIGIN      
        1 tttcataata atttgtgttt atatcgtttt atatggataa attaagggtt attttgaggc
       61 aaatttaagt actcaaatca acaataaatc gacggataaa ttcacttcaa gtaaaataat
      121 tcgtatactt tcttaagtca ttaaattaat taaataaact tcaaataaac gcggagtgta
      181 gctcagactg gtagagcact gccttcggga ggcaggggtc gcaggttcaa ttcctgtcac
      241 tccgaccata taaataaagg attataagca tttttacaaa accaaaattc aattataggg
      301 tgcgcacagg gtgcaaattg aatgtgtttt attgaaaaaa gaatatatct ggaatcatgt
      361 gtaagatatt aaaatcaatc tacattagct taatgttgat agtttcccct cagataaaag
      421 cagcctcaga tacatgccat cccaaaataa acccaagatt accccaatat attgctggtt
      481 atggaagttt gattgatgag caatctaaaa agactacaga ctcaaaagct tatgaaaaca
      541 ttcctgtttt aattaaaggc tttaaaagga gttggtgtgc acatggtaat atgccaggat
      601 tcaatgcaac ttttttgtct ttaaatgaaa acaaaaaagg gacatttaac ggcgtaattt
      661 atagagtcag tgatcccaaa gaaatagagc tatatgacac tagagaaact gtttattgta
      721 ggaaagagtt aagtgccgaa caaattaaaa tatatacaaa aaaaattcct gaaaaaatac
      781 aggtatggat ttactatcca aaatcaacac aaaatgatca acctagccgt gagtttccca
      841 ttacacaatc atatgtcgat atttttatta gaggttgcat taaaattgaa gaaaaattta
      901 atataaaaga ttttgctaaa gaatgtatat taactacgga taactggcct gaacaacact
      961 gggtcaatga tagagtttat cctagacgcc cttcagcata tgagccttat gcacgtaaga
     1021 tagatgctct attaaaagaa catttaccta agcaatttaa aaatataaca atcgaataaa
     1081 ttgtagggtt agcgcaaaag cattattgcg ctaactcaat cagtagatac tttaacgcac
     1141 taaccacact ttctgtgctg ttctagccat gaatcaaggt catccttcaa atagcgtatt
     1201 gatcgtccga ttttaatata tttagggcct ggagttctat ttgctaaagt tccatttacc
     1261 ctgtcttgag aaagaaaaga acggctcatg catatatagt tagcagcttc agcttcttta
     1321 aagactctct tctccacaat aaatacctcc gtatattttg gtgtaacatc ataaaacaac
     1381 accaatatat accaattata agtatttatc aattaaatta tacgcgattt acgtattcat
     1441 taacggcagt gataacagct tcaacttcaa aagttcggcg agtggctgta aatctcccct
     1501 taaatcgatt catcccatcc cctctcattt ttggcgcgtg ttcctcaaaa taattttgca
     1561 aatcctcggc actttcaagt tgatattgaa cagttaaatg tttttgatta tcagagctat
     1621 caccatctat tgattggtgc atcactgttg cattcaaaaa accgggaaat tttagcattt
     1681 caagaatatg ctcgtctaac caatccttat aatctctata tatttcatta tcaatagata
     1741 gattcacttc atatacaacc atgtttactc cctcacaaaa cgtaatacaa ccaatgttaa
     1801 tatcgcacaa atactaataa caattcccgg attcataaaa cttttatggc ttaatataac
     1861 atagggcatt aaaccaccaa aaattgataa gctggtatta taagatattg ctaaagcctt
     1921 acctcgatga atggcgggaa acaaagaaac cataaatgca ggcaaagccg aattatataa
     1981 tgattgaatc aaagccagta atatcaaact acaaaatata acgacatagt taaaatggct
     2041 aatatagtca aaacagacat aggaaaataa aaggtataat gctgaagcag cagtaatttg
     2101 cttagtaata cctacccgat cggccaaaga accaaacagg ggcaaacaga tgaaataaac
     2161 tgacaaagca atcaacgtca cccatacaga tttatggtga gtctgatttt gaatcactga
     2221 agataaaaga gttggcatat aaacaaaggt catatagaaa gcagaagcag ataaacttgc
     2281 agctacaaaa caaataacca gctcctttct atattttttt accaaagatg aaaaatgttt
     2341 agtatcattc atcttctgcg caaatggctc attaataaac cttctaatct ggtagagtgc
     2401 cagccaaata atagcactaa ttaaaaatgg tatacgccag ccatattgaa tcatctgctc
     2461 ataaggtaaa aggtttagca atataaaaac cgataaattt gccagcaaca atccaagaat
     2521 tccgcccata aatgccatac tgccagaaaa tgccggacga tctttccctt gttcaacagc
     2581 aaatattaaa gcattagaaa actcccctga gattgatagc ccctgaataa tccggcaact
     2641 aaataatgct atccataaat caatgccagg taggctggtg ggcaataaac caataaccgc
     2701 tgtggaaacg gtcatcaaca aagtagtaac tgacagtacc tgctttcgac cataactatc
     2761 agacagatac ccaaaaacca ttccagaaaa gggtttcact acatagctta ctacataagc
     2821 caaaatggta aaattaatcg ctgaactttg tgaatgggat gagaaaaata cggaagacaa
     2881 cacaggaata agaaaagcaa agatacttaa atcaaaaact tcaatagcag tactgatgaa
     2941 aacggatatc ctgaccagat ttgtcattta cattcacttc attgtgtcgt acattatttt
     3001 agcattaaat agatttaagc tgtacctcta aggatttgtt atgatacatg atatacccaa
     3061 aaggattttc cttcccttcg atgaaaaaca agaacttctt ttcaacaaac aattacgtcc
     3121 cctaaatgcc ttggggtgtg attatttcta ttatctagtc tcagatcaaa cttcaaaaag
     3181 caaatcctac cgattctgca ctcatgaaga ttggatggct ttctattatg aagaaaaatt
     3241 aatcgaaaat gatccattaa aaagaattgt agaaaagtca aacatttcag tactcccatg
     3301 gaatcaagcc agttttatga ataagcatga aaaacaaacc atgtctggcc gatccgcttt
     3361 tgggctttat aatggtgtct cgattgttag taaattcaat aacaaaaatt acatatttgt
     3421 attggcaact gaacatagag atcatgactt agcaaggtat ttgctattgg aaaaaaatta
     3481 tgaattaaag aaattaatgc atgggtgtat agcctctttc gaaggatgac gtggttaggg
     3541 cttatgattc tgattgattt acaagagcat tgaataaatt aaccaacttg agctgttcaa
     3601 ccctatttac tgtaacagca caaagctcgg gattgccaac tactattgct aaactttgag
     3661 ctcttgaaat ggcaacgtta attcgatgct tatcgaagag aaaatctatc cctctaggtg
     3721 attcattcgc atcgctggta cacatactca aaaatacaat gggcgcctct tgcccttgga
     3781 atttatcaac actgccaaca cgtgcctgct catcaagagc ttgcttgagt ttattaactt
     3841 gataattata tggagcaaca aacaatatat cattccaact aattttacga gtttctccca
     3901 gcttttctat aaatgtcctg cccattaatt cttgagctaa tagatttatt tcactgactt
     3961 cctcatctga agcgtaggta ttaccttcat gctccacagg aacaaagatt atgccagcct
     4021 ctttgtttaa ttttccttgg taaccatcag gtactttaac aacacgcaca tcattatcag
     4081 gatgtggctc aaggttacta tcatatataa atttactgat aaagtgattg actgttgaat
     4141 gcattcgata agtggtacct aaaaatactc ccatatcatc ggcaatcgta ggtgtttcat
     4201 gtaataaata atctaaaata gaaagaccgc tttcaccagg atgtgtgcct tgtgatggct
     4261 gtcctaattg catttgatca cccataatca ctaaattctt agcagagcgg ctcatagcaa
     4321 ccaaattagc cactgaaacc tgccctgctt
//
LOCUS       DATNSN010000209         4280 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.210, whole genome shotgun sequence.
ACCESSION   DATNSN010000209 DATNSN010000000
VERSION     DATNSN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.210"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            235..2550
                     /locus_tag="VAH76_002875"
     CDS             235..2550
                     /locus_tag="VAH76_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO1387456.1"
                     /translation="MKENVSHLPRELSRMVFWSLTCLKKAIAEVYGKETYERIEQIRL
                     SMQDTIGSESFSLGKALHSLQIELSKLEKKQLYQIAHGFSLMMELINTCENAYRIFRI
                     NQRKEQVYSDMPQGIHYVLTAHPTEARTNEFLKLFKEIRDYLIEVLKSPYLMNESHLD
                     QMLKISLQINISYHEKPSVEDEAQYIYQYALHPLNIKLYHSFLAKGVPIQLRTWVGGD
                     KDGHSGVNEKIMLKSLELSRSFFIAYIQECLREVLEIIKLSTSLDKNKPLADQINQLI
                     KKAYGLKKIQSKEHEKISIFKNELTATQINTLKFLNFEPEQFSIISSIFHLYPALVMP
                     IELREDSAVVKESLTSIKKTTITKMLEQVHSITHGTDPKNYIRGFILSMVESAEDIKN
                     GINLVKKVFKSYALPVVPLFENQLALTNADTILNNSITEDIRQKHLKQWDGNYEVMLG
                     YSDSSKENGVLPSRLMIAKSLKNIQSTLENKNLRPIFFHGSGGSIERGGGDIKEQTAS
                     WSKEIMSNYKVTVQGEMVARLFGSSSILKSQIDKFLDIYSQKGKSNDNDYPEELSLFS
                     TRVSQKYKTLIQSDWFWQIVEQASPYHFLKELKIGSRPTKRKSGPDQRKLRAIPWILC
                     WTQTRLLFPTWWGIGSTWSELNGIEKNRLKALYKENALFAAFVKQLGVTLSKVYLPVW
                     EQYLLQLTGSIEYLRPFQSELESTIQFFYQITGEKDFCFHQPWLGESIQLRSTLIHPL
                     NLIQIEAMKRKDLPLLRKTVTGISCGMLTTG"
     gene            2703..3185
                     /locus_tag="VAH76_002876"
     CDS             2703..3185
                     /locus_tag="VAH76_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO1387457.1"
                     /translation="MSFSMKINNIPYYVATRVVKRIQSWLGLNTLGARAIVTNTEGHV
                     LLVKHTYQPHWYLPGGGVKKGESTKAAVIRELHEEVGLVVAEQDVILFGIYHHKYLGV
                     NDYPVIYIVKNYTSHVTHSREIEQMGWFCLDALPEMVSPGTKRRLGEYFDNNPISEKW
                     "
     gene            complement(3236..>4280)
                     /locus_tag="VAH76_002877"
     CDS             complement(3236..>4280)
                     /locus_tag="VAH76_002877"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO1387458.1"
                     /translation="ERILSLANDVRKVADLPNPLGVNLLEKSMPNGLSIKKITVPLGV
                     IAVIYESRPNVTIDIFSLCFKSGNVCILKGGKEAHFTNSYLLLLIKNTLKNFNINTDI
                     VCLLPPERALMTQLLNATGLVDLCIPRGSQNLINFVRDNAKIPVIETGAGIVHTYFDK
                     SGDLGKGKKIINNAKTRRVSVCNALDTLIIHADRLKDLPELVETLSQKNVIIYADQDA
                     YQVLDKNYPKQLLIKAKPQDFGHEFLDYKLAIKTVPNIKAAIDHIQQFSSHHSEAVIA
                     EDESAIDKFLTEVDAAAVYANASTSFTDGGEFGLGAEIGISTQKVHARGPMGLDALTS
                     YKWVIRGTGQIRN"
BASE COUNT         1414 a          811 c          770 g         1285 t
ORIGIN      
        1 atagtattct ttataggaca ttgattttgg tctgccttaa aaaataaaaa tattttccaa
       61 cacaaattaa gtatattaaa attggatgcc aaagtattct tgaatagttg tgccaaaatc
      121 ctttgaggat caactagaga agtagacaat gaagtattta ctgcatcaga gttagataac
      181 gaacagacgg cattcctcta cagaataaac cattcggata aaatggagtt ttcgatgaaa
      241 gaaaatgtgt cccacctacc aagagagctc agccgtatgg ttttctggtc acttacctgt
      301 ttaaagaaag ccattgcaga agtctatgga aaagaaacat atgaaagaat cgagcaaatt
      361 cgactatcca tgcaagacac gatcggaagc gaatcgtttt cacttggaaa agctcttcat
      421 agtcttcaaa tcgagctttc caaacttgag aaaaaacagt tatatcaaat agctcatggt
      481 ttttccctta tgatggagct gattaatacc tgtgaaaatg cctaccgcat ttttcgtatc
      541 aaccaaagaa aagaacaagt ttattctgat atgcctcaag gtattcatta tgtattgaca
      601 gcacacccaa cagaggctcg taccaatgaa tttttaaagc ttttcaaaga aattcgtgac
      661 tatcttatag aagttcttaa atccccctat ctgatgaatg aatctcatct tgatcaaatg
      721 cttaaaatct cacttcaaat taatatttcc tatcatgaaa aaccaagcgt ggaagacgag
      781 gctcaataca tctatcagta cgcactccat ccacttaaca tcaaactgta tcattcattt
      841 ttagcaaaag gggtccctat tcaattacgc acctgggtag gaggtgataa ggatgggcac
      901 tctggtgtta atgaaaaaat catgcttaaa agcctagaac tttctcgttc tttttttatt
      961 gcctacattc aggaatgctt aagagaagtg ctggaaatca ttaaactttc aacttcatta
     1021 gataaaaata aacccttggc agatcagatt aatcagctta ttaaaaaagc ctatggtctt
     1081 aaaaagatac aatcaaaaga acatgaaaaa atatcgatat tcaaaaatga attgactgct
     1141 acacagataa ataccctaaa atttttaaat ttcgaacctg agcaattctc catcatctcc
     1201 agtatttttc atctgtatcc cgctttggtc atgcctatag agcttagaga ggactcagct
     1261 gttgtaaaag agagtttaac aagcataaaa aaaacaacaa ttactaaaat gcttgaacag
     1321 gtacactcta taactcatgg gactgatcct aaaaactata tacgcggttt tatccttagt
     1381 atggttgaat cagctgagga tataaaaaat ggaattaatc tggttaaaaa agtttttaaa
     1441 agctatgccc tgccagttgt gcccttgttt gaaaatcaac ttgccctcac taacgcagat
     1501 acaatattaa ataattcaat cactgaggat atcagacaga agcatctcaa acagtgggat
     1561 ggaaactatg aagtcatgtt gggttactcc gactcgtcca aagaaaatgg cgtactgccc
     1621 tctcggctta tgattgcgaa aagtttaaaa aacattcagt ctaccctgga aaataaaaac
     1681 cttcgaccca tcttttttca tggttcgggc ggtagtattg agcgaggagg cggggatatc
     1741 aaagaacaaa cagcctcctg gtcgaaggaa ataatgtcta attataaagt gactgtccag
     1801 ggagaaatgg ttgcaagact ctttggttca agttccatac tcaaaagcca gattgacaaa
     1861 tttcttgata tctattcgca gaaaggcaaa agcaatgaca atgattatcc ggaagagttg
     1921 tccttgtttt cgacaagagt tagccagaaa tataaaacac ttatccaaag tgactggttt
     1981 tggcaaatag tagaacaagc ctccccttat cattttctga aagaattgaa aattggctct
     2041 cgtccaacca aaagaaaatc aggccctgat caacgaaagc ttcgcgccat cccatggatt
     2101 ctgtgctgga ctcaaacacg gcttcttttt ccaacctggt ggggtatagg ttccacttgg
     2161 tcagagctaa atggcattga aaaaaacaga cttaaggctc tttataaaga aaatgctctt
     2221 tttgctgctt ttgtaaagca attaggtgta accctctcca aggtttatct acccgtctgg
     2281 gaacagtacc tgcttcaatt gactggctct attgaatacc taaggccttt tcaatcagaa
     2341 ctagagtcaa ctatccagtt cttctatcaa ataaccggtg agaaagattt ctgttttcac
     2401 caaccctggc ttggggaaag tattcaatta cgatcaacat tgattcatcc tttaaatctt
     2461 attcagattg aagccatgaa aaggaaagac ttgcctctct taagaaaaac cgtcacaggc
     2521 atttcttgtg gtatgttgac aacaggataa tagaagtaaa caacgttatc aatgccactc
     2581 taacagaggg caatgttatt gtagaactaa tgaaatttga acttaaaaga tatccagtga
     2641 ccgtcatggc gagtattctg taaagaatag ggataagttg agaaatataa aatatacaat
     2701 aaatgagttt cagtatgaag ataaacaata tcccttatta tgtcgctacc cgtgtcgtaa
     2761 aacgtataca gtcatggctg ggattgaata ctctgggggc acgagcaata gtgactaata
     2821 cagagggaca cgtgttattg gtaaaacata cctaccaacc tcattggtat ttaccgggag
     2881 gcggagtaaa aaaaggagaa tcaaccaagg ctgcagtaat ccgtgaactg cacgaagaag
     2941 ttggtcttgt tgttgcagaa caagacgtga ttttattcgg catttatcac cacaaatatc
     3001 ttggagttaa tgattatccg gtaatttata ttgttaaaaa ctataccagt catgtgactc
     3061 actccaggga aattgaacaa atgggatggt tttgccttga tgcattaccg gaaatggtga
     3121 gcccgggaac caagcgcaga ctgggtgaat attttgataa caatccaata tcagaaaagt
     3181 ggtgattatt gcagttgtca ttattggaga cggcgttggg aaaatagaat tgtgcttaat
     3241 ttctaatttg tcctgtccct cttatcaccc atttgtagct tgttaaagca tctaatccca
     3301 taggccctcg cgcatgaacc ttttgcgtac taattccaat ttcagcccct aatccgaact
     3361 ccccaccatc tgtaaatgaa gtagatgcat tggcatagac agctgcggca tccacttcag
     3421 ttagaaattt atcaattgca gactcatctt cagcaataac agcctcacta tgatgggaac
     3481 tgaattgttg aatatgatca atagctgctt ttatattggg tactgttttt attgctaatt
     3541 tatagtccag aaattcatgg ccaaagtctt gtggttttgc cttaattaaa agttgtttag
     3601 gataattctt atctaaaacc tgatatgcat cctggtcggc atagataatc acattctttt
     3661 gagacaaggt ctccacaagt tctggcaaat cttttaaacg atcagcatga ataatcaaag
     3721 tatccagcgc attgcaaaca cttactcttc tggttttagc gttgttaata atttttttac
     3781 cttttcccaa atctccactt ttgtcaaaat aggtatgcac gatacctgcc cctgtttcaa
     3841 tgacaggtat tttggcattg tctctgacaa aatttattaa attctggctc cctcttggaa
     3901 tgcataaatc aacaagacct gtggcattta ataattgagt catcaatgct ctttctggtg
     3961 gtaataaaca aacaatatct gtgtttatat taaaattctt taatgtattt ttaattaata
     4021 aaagcagata actattagta aaatgtgctt cttttccacc ttttaaaatg caaacatttc
     4081 cagatttaaa gcatagactg aagatatcaa tagttacatt aggtcttgac tcataaatga
     4141 ctgcgatgac acctaaaggt actgtgattt tcttaatact caaaccattt ggcattgatt
     4201 tttcaaggag gttaactcct aatgggttag gtaaatcggc tacttttctc acatcattcg
     4261 ccaatgacaa aattctctct
//
LOCUS       DATNSN010000210         4181 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.211, whole genome shotgun sequence.
ACCESSION   DATNSN010000210 DATNSN010000000
VERSION     DATNSN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.211"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            92..343
                     /locus_tag="VAH76_002878"
     CDS             92..343
                     /locus_tag="VAH76_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO1387459.1"
                     /translation="MSKNNVSRIQLINEFESAPESTLFNQNTLAAVLDCSVQLLERNR
                     WAGQGVPYLKIGRTVRYRKSDILNYLQQQCTYNVLQDAV"
     gene            420..2174
                     /locus_tag="VAH76_002879"
                     /pseudo
     CDS             420..2174
                     /locus_tag="VAH76_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            2540..2773
                     /locus_tag="VAH76_002880"
     CDS             2540..2773
                     /locus_tag="VAH76_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387460.1"
                     /translation="MKAKFLLLGFCCFSLLSISVSYAKRGYSYSYKTPRYSSSYGRTH
                     TVKPYIKRDGTFVKRHRAGNPKAGVHCHNNVCY"
     gene            2793..3200
                     /locus_tag="VAH76_002881"
     CDS             2793..3200
                     /locus_tag="VAH76_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387461.1"
                     /translation="MFLDKKIILMVLSIIIKPTYSAGIYTGKDFIFACTDQNYTKNQD
                     VCNTAITQAFATYMVSLELFSGEKLAKCYRNHYPFLEKKSVKDGVQFLTEQYKENPEL
                     TPHLLGFGFSVVMYSKYPTPRKCIKFKQSGVSI"
     gene            3197..3418
                     /locus_tag="VAH76_002882"
     CDS             3197..3418
                     /locus_tag="VAH76_002882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387462.1"
                     /translation="MKILKTLSLLMGVILAVSQLQGCAVAAIGAGIGAVKYANAKKAE
                     AKEGCHKNYNEYLRVAKKPIPLSEYCSEK"
     gene            3581..3907
                     /locus_tag="VAH76_002883"
     CDS             3581..3907
                     /locus_tag="VAH76_002883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387463.1"
                     /translation="MKKAIAVSMLAMTMSCHAFNLISDTDANRFKNMSSNSIKPDQFN
                     KLMAFCDKTDSNIESCLEKIKAKSSDNKSMQSNVTQGLCCWNVSNQYGDYLYYQGKEY
                     CIPACH"
BASE COUNT         1339 a          703 c          853 g         1286 t
ORIGIN      
        1 tactggaatt ttcaaattgg atttatggaa aaagcctctt tggcggcatt ttttaatgtc
       61 gattgaaaat tcttttaaga aaggtatcgc tatgtctaaa aataatgtat cgcgcataca
      121 acttatcaat gaatttgaat ctgctccaga atctaccctc tttaatcaaa atacacttgc
      181 cgcagtttta gattgctcag ttcaattact tgaacgtaac cgttgggctg gtcaaggtgt
      241 gccctactta aaaattggtc gtacggttcg atatcgcaaa tcagatattc tcaattatct
      301 tcaacaacaa tgcacttata acgtccttca agatgctgtt tagcagctct ttgattaagc
      361 cttgattgat tggaaggtgg tggaacacct tcctgtgaac gttgttggag aaatagaaaa
      421 tgaattcaaa tcaaatatat catgtcgatt ttaaacagtc taaacgtatt aaatcgtttc
      481 cctatgctgg tggaagcttt caattaacct cagatggggt aatttttatc ggtaaagata
      541 aagataataa tgagcttcct cctcgatgga tctgctcttc tttgtctgtg gtagctaaaa
      601 ctcgtgatgc caatagtggg gaatggggtc gattgcttga atgggtcgat gatgatggag
      661 ttaaacatca atgggctatg cctttggctt tattgcaagg ggattcaagt gatgtgaggc
      721 gagaactggc acgattgggg ttgactatat cacctagtaa atctgctcgt gatttattag
      781 cttcttatat ccaagttttt ccagtcgaag agcgagctcg ttgtgttgat aaactgggtt
      841 ggtataaaga cgtatttgtt actgctaatg aagccatagg gcaatccagt gaccaaatcg
      901 tttttcaaaa cgcgaatagt cttgaacccg cactgtctgt tgcgggcagt ttagaggact
      961 ggcgaaattc tatcgcacga ttggcagatg gtaattctcg cttggtattt gctatttccg
     1021 cagcctttgc accaagcctt accaatcttg ttggtgagga ctcagggggc tttcatttca
     1081 gaggggcatc ttcttctgga aaaacaaccg ctttaaaagt agcggcttct gtttggggta
     1141 agccagatag ttatatccgt ttgtggcgaa ttacagcgaa tgggttagaa ggattggcag
     1201 ccctgcataa tgatggatta ttaatcctcg atgagttgag tcagatggat cccaaagaag
     1261 ctggtgagtg tgcttatcta cttgcaaatg gtcaaggtaa gacaagagcc tctcgttgtg
     1321 gtacggcaag acaatccatg cgttggtcat tactcttttt atcagccggg gaggagtcat
     1381 taacctcgtt aatggccaaa gcaggacaac gatgtaatgc aggtcaagaa atacgtttag
     1441 ccgatattga agcagacgct ggtgtaaaga tggggttatt tgaacaatta catgatcata
     1501 taaacccagc ctccatgtca ctttcgttaa aagaggcagc cagtcaatgc catggtgctg
     1561 ttggtgtagc atggctccat aaaattgtga aacaccgagc agagttaatc tccctactca
     1621 ccaataaaat ccagcaattt gtaactaaag tcacaaagcc cgaacattca ggtcaaattc
     1681 aacgagtagc aagacgtttt gctttggttg ctatggctgg tgaattagcg actcactatg
     1741 aactgactgg atggaaatcg ggtgctgcat gtcaagcagc agaaaagtgc tttaacacct
     1801 ggctagaaga ttttggagaa catggaaatc gagaagatcg agccatattg tcataagttc
     1861 gtgcattctt tgaaaaagaa ggagcaagtc ggtttgaaag cgaaaatcat cctaatagtg
     1921 aacgacttta caatcgagct ggtttttatc gaactgatga cgaaggtttt cgaattttta
     1981 tggttttatc tgaagtctac aggaaagaga tttgccaagg atttgaacct aaaatggtga
     2041 ataaagtgct tattaatgct ggctggatag ttcctggcaa tgacggcaaa gcatctcaaa
     2101 aacgaagaat taaaggtatt ggtattccta gattttatat tttcactgag aaagtttggt
     2161 ctgacgacta ttaattgtcc cactcgttcc actgtcgatt ttgatagtgg aacatccaaa
     2221 gtccagtaac tactagagtg ttccacttgt tccactagtt ccacgtattt ttagatgcag
     2281 gtaaaaatct attaggttgt ataggcatta aggaagatga tttcccatat tacgacttcg
     2341 tctaatacgg actacgcttg ttaagagcaa tcttaaattt ttgcttgaca agatactaat
     2401 gatttcttac catccggcct ctgtgattaa tatatgatca tcaggttaat ttgttatcat
     2461 ccgacaagta attttatttt actttaaatc aacaataaga attcggctct gtaactcatg
     2521 ttttaataat gaggctatca tgaaagcaaa atttttattg ttaggatttt gttgtttttc
     2581 tttattatct atatctgtaa gttatgcaaa acgcggctac tcctattctt ataaaactcc
     2641 aagatacagt tcttcttatg gaagaacaca tactgttaaa ccttatatta aaagagatgg
     2701 aacttttgtt aaaaggcatc gggctggaaa ccctaaagca ggtgttcact gccacaataa
     2761 tgtttgttac taacactata gtggatggcg ctatgttttt agacaaaaaa ataattttga
     2821 tggttcttag tattattatc aaaccaactt attctgcagg aatatatact ggtaaagact
     2881 ttatttttgc atgcacagat caaaattaca ctaaaaatca agacgtttgt aatacggcta
     2941 taactcaagc attcgcaacc tatatggtat cccttgaatt attttccgga gagaagttag
     3001 ccaagtgtta tagaaatcat tacccattct tagaaaagaa atccgttaag gatggagttc
     3061 aatttttaac tgagcaatac aaggaaaatc cggagttaac tccccatcta ttgggttttg
     3121 gattttcggt tgtaatgtat tctaaatacc caaccccaag aaagtgcatt aaattcaaac
     3181 aatcaggtgt atcaatatga aaattttaaa aacgttatct cttttaatgg gagtaatttt
     3241 agcagtatca caactacaag gatgtgcagt agcagcgatt ggtgctggca ttggtgctgt
     3301 taaatatgca aatgcaaaaa aggcagaggc aaaagaaggc tgccataaaa attataatga
     3361 atatttgagg gttgcaaaga aaccaatacc tctttctgaa tactgttctg aaaaataaca
     3421 gtaaagaatc aattaaacca acgtcgaaac aatagacaat aaacttttaa ctataaccct
     3481 agtgaaacta aagagtttta aggtatttga gtgggtaaag gattaatcac tggtgggcta
     3541 gatggagatt tttacaatta actgagaggt agatgaataa atgaaaaaag caatagcagt
     3601 atcaatgtta gcaatgacca tgagttgtca tgcttttaac ttaatttcag acacagatgc
     3661 aaatagattt aaaaatatgt ctagtaatag cattaaacca gatcaattca ataaattgat
     3721 ggcattttgc gataagactg attcaaatat agaatcatgt ctagagaaaa taaaagccaa
     3781 gtcatcagat aataaatcaa tgcaatctaa tgtgactcaa ggtctgtgtt gttggaatgt
     3841 atcaaatcaa tatggtgatt atctgtatta tcaaggaaaa gagtattgta ttcctgcgtg
     3901 tcactaatat aagtatataa aaggcggcta aagtcgcctt tgtattcatg atcgaattat
     3961 tttgtcatct atttatttta agacattcct tacaggacgg attctgaaaa aagaaaaaat
     4021 caatcagttt atttctatca aaacttcgac acatattcga cacgcaatca aaaacaagtt
     4081 ggggaattct aatctgaagt gcgacagtcc aaaaagctgt atcatgttct tggggaactt
     4141 gaaatagcaa aggagactgc cacatgggac agtatactca a
//
LOCUS       DATNSN010000211         4129 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.212, whole genome shotgun sequence.
ACCESSION   DATNSN010000211 DATNSN010000000
VERSION     DATNSN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.212"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..423)
                     /locus_tag="VAH76_002884"
     CDS             complement(<1..423)
                     /locus_tag="VAH76_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387464.1"
                     /translation="MNKCSGAIILLSFPHQLIHALAALFTDRERRRINKDAPILIFIW
                     SYRWFDHRKNSPFLEIIKKLSKIENCQLYIPSLLQRVFGLSEFRVLKQRVKYVNKIIS
                     LKDYDSFYYSHDFSADHSAQVFMQAFPNIYKVCFGDPPG"
     gene            complement(420..1853)
                     /locus_tag="VAH76_002885"
     CDS             complement(420..1853)
                     /locus_tag="VAH76_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO1387465.1"
                     /translation="MSWSIRIENLSKQYRIGSTTTTAAELINEKLRKFLLLLDFKKRN
                     RFSSNKPLPSSIAQEGRTVVDASQISPEYPNHFWALRDINLEIEAGERLGIVGQNGCG
                     KSTLLKILSRIVSPTEGLFRFRGRLISLLEIGTGFHGELTGRENIFLNGTIMGMKTHE
                     IKNRLNQIIEFSELGSMIDTPVKRYSSGMYIRLAFAVAAHLESEILIVDEVLAVGDAA
                     FQRKCTEKMLDLANQGRTLLFVSHDMETVNRICNRAIRMSHGRIVGDSKFDTSVASKS
                     SVMDITRDYLRSGAKYKSEQVWPSDKPMIFLNSLKLNRVRLIDYDNKVRSNFNLNETI
                     IVEIDFTIIKETYPFNIHLYLKDSSGRVIFVSMDNHAWQSKKVRPLGNYTERCTLYSP
                     LLNIGDYHIDIEFWPGNALENRLVVNSVVSFELKDGAVNEGVRGNWAADWPNSLIRPQ
                     LDWSISTNNKKHLVLKEHELVTEKQSI"
     gene            complement(1850..2701)
                     /locus_tag="VAH76_002886"
     CDS             complement(1850..2701)
                     /locus_tag="VAH76_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1387466.1"
                     /translation="MTSISSKTQTFKETVVYGVDKFRLLAVLRNIWDYRLLLWIFCKR
                     DLKGRYSQTILGLGWVILTPLITVGVFVIVFGIMIKVPTDGLPTVMFYLVAVIPWYSF
                     LNVLNPSIQMIEGNASLITKVYFPRALIGGAYAMGAAVDFLMAYVFLITPFAIYYGLW
                     SIKLLIIMPFLLISTLMIGLGIGLVLAPINAKYRDIKHFMPLALQLFYYSTPAIYPVS
                     AVPVWAKPWYAVNPLSLVITSYREVLMGHWPSPTIIMTLSGMAIITFLVGLVAFHHME
                     HKVVDIL"
     gene            complement(2667..>4129)
                     /locus_tag="VAH76_002887"
     CDS             complement(2667..>4129)
                     /locus_tag="VAH76_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387467.1"
                     /translation="SLNIKSKSGHESSSSKSYPVESQLDNGYIPYSFLLKSKRRSSFL
                     FYPILFLIGFLCIFIVAIKIFSKILKRIFSNISKFFNISKFFGGLLKNNSLFQITSGV
                     LKNFGYAYRVLKLHHYDLLIFGQEFPGSINSLLIKLCNKRNTPTLIIPFALGTTKEIV
                     ESLYDKDIFDVSSSITNYLASLLYPNWINFYKGKKMLRLTGKQIFFLELMGLAPEHPW
                     LPNNSFVTKIAVESEKMFSYYQSMKFPVHQLSLTGSLSDDILVEATTNGQEIKKKICQ
                     KMNLDPEKPILLCAWPTDQFGSRFVSLEFKNYEQLCRAWAKCLKEIEREGKYSVIIRP
                     HPVTNKEILGKILGQCDLKQFISYDDTIQLITCCDLFVACVSSTLRWAIAKGIPAINY
                     DCYQYGYTDFDAKGVFNVNDFKSFSAVLEELTRDRQKYEGAKQNQQECASEWGILDGN
                     SQKRIVNLINKLVQSESVIDIENKNYDLNILKNSDV"
BASE COUNT         1398 a          791 c          646 g         1294 t
ORIGIN      
        1 cccaggtgga tcaccaaaac aaactttata aatatttgga aaagcctgca tgaatacttg
       61 cgcactgtga tcggcactaa aatcatggga ataataaaag ctatcataat cctttagtga
      121 aattatttta ttaacgtatt ttactcgttg ctttaatacc cggaactcag ataatccaaa
      181 cactctttgt agcaaactcg gaatgtaaag ctggcaattt tctattttcg ataatttctt
      241 aattatctca agaaatggtg aattctttct atgatcaaac caacgatatg accagataaa
      301 tatcaaaatt ggagcatctt tattaattct acgccgctct ctatcagtaa ataatgctgc
      361 aagagcatga attaattgat gggggaaaga aagtaaaatg attgcaccac tacatttatt
      421 catattgatt gcttttcagt gactaactca tgctctttta aaacaagatg ttttttgtta
      481 tttgtcgaaa tgctccaatc caactgtggt ctaatcaatg agtttggcca atcagcagcc
      541 caattgcctc ttaccccctc atttacagct ccatctttca actcgaagct gactactgaa
      601 ttgactacca atcgattttc caaagcatta cctggccaaa attcaatatc aatatgataa
      661 tcgccaatgt ttaataaggg tgaatataaa gtacatcgtt cggtataatt tcctaatggg
      721 cgtactttct tagattgcca ggcatgatta tccattgaca cgaatataac acgcccagag
      781 ctgtctttaa gataaagatg aatattaaaa gggtatgttt ctttaataat tgtaaaatct
      841 atttcaacaa taatagtttc atttaaatta aaattagagc gaactttatt gtcgtaatct
      901 atcaatctaa ctcggttaag cttaagggaa tttaaaaata tcattggctt atcactaggc
      961 caaacctgtt ccgatttata tttcgctcct gagcggagat agtcacgagt gatatccatg
     1021 actgaacttt tgcttgctac actggtatca aatttgctgt caccgacaat tctgccatgc
     1081 gacattcgta tggctctatt acaaattcga ttcacggttt ccatatcgtg actgacaaat
     1141 aataaagttc ttccctgatt cgccaaatcc agcatttttt ccgtacactt gcgttgaaat
     1201 gctgcatcac ctaccgctag cacttcatca actataagaa tttcagactc caaatgagca
     1261 gctacagcaa atgccaagcg gatatacatt ccggaagaat agcgcttgac aggagtatct
     1321 atcattgatc ctaattcaga aaattcaata atttgattga gtcgattttt aatttcatgg
     1381 gttttcattc ccataatagt gccatttaaa aaaatatttt cacgtccagt cagttcacca
     1441 tgaaatcctg taccgatttc caacaaactg attaagcgcc ccctaaatcg gaataaccct
     1501 tcggtaggag aaacaatacg tgatagaatt tttaataatg tgctcttacc gcagccattt
     1561 tgtcctacta taccaagccg ctcccctgcc tcgatttcaa gattaatatc tcgcaaagcc
     1621 cagaaatgat tgggatattc cgggctaatt tgacttgcat cgactacagt tctaccttct
     1681 tgtgcaatgg atgaaggcag tggcttattg gatgaaaatc gattcctttt tttaaaatcc
     1741 agtaataaaa gaaattttct caatttttca ttaatcagct ctgctgcggt tgtagtagta
     1801 ctaccaatcc gatattgttt ggaaagattt tcaatacgaa tagaccagct cataaaatat
     1861 caactacctt atgctccatg tgatgaaatg caacaagccc cacaaggaaa gttataattg
     1921 ccataccaga taatgtcatt attatagtag gtgatggcca atgccccatt aacacctctc
     1981 gataactggt gataacaaga gataatggat taacagcata ccatggttta gcccacacag
     2041 gcacagcaga aacagggtaa atcgctggag tggaataata aaataactgc aaagccagag
     2101 gcataaaatg tttaatatcc ctatattttg cattgatagg tgctaaaacc aaaccaatac
     2161 ctaatccaat cattaaggta gatatcagca ggaatggcat aataattaat aacttgatag
     2221 accaaaggcc ataataaatt gcaaaggggg ttatcaaaaa cacataagcc atcagaaaat
     2281 caactgcagc tcccatagca taggcaccgc ctatcaacgc tcttggaaaa tagacctttg
     2341 ttattaacga agcatttccc tcaatcattt gtatggaagg attgagaaca tttaaaaagg
     2401 aataccaagg tataaccgca accaaataga acatgacagt aggcagccca tcggtaggta
     2461 cctttatcat gatgccaaaa acaatgacaa acaccccaac cgtaatcaaa ggagttaaaa
     2521 taacccaacc caatcccaag atggtttgac tgtaacgccc tttgagatcc cgtttacaaa
     2581 aaatccataa aagcaagcgg taatcccata tatttcttaa aacagctaaa agcctaaact
     2641 tatcaactcc atagactact gtttctttaa acgtctgagt ttttgaggat attgaggtca
     2701 taatttttgt tttctatatc aattacagat tccgattgaa ctaacttatt aattaggttc
     2761 actattcttt tttgggagtt tccatccagg atgccccatt cactcgcgca ttcttgttgg
     2821 ttctgtttgg ccccctcata tttctgccta tcacgcgtta attcttccaa aacagcagaa
     2881 aagcttttaa agtcatttac attaaaaaca ccttttgcat caaaatctgt atatccatat
     2941 tgataacaat cataattaat ggcagggatt ccttttgcaa ttgcccaacg caaggttgat
     3001 gaaacgcaag ctacaaataa atcacaacat gtaataagct ggattgtatc atcataggat
     3061 ataaattgtt ttaaatcaca ctgtcctaat attttaccta aaatttcttt attagtaacc
     3121 gggtgaggtc taataattac ggaatatttt ccttcccttt caatttcttt caaacatttt
     3181 gcccatgctc tacaaagctg ttcgtaattt ttgaattcca gggaaacaaa tcgtgaacca
     3241 aattgatctg ttggccaagc acacaataaa ataggttttt ccggatcaag gttcattttt
     3301 tgacatattt ttttcttaat ttcctgaccg tttgttgttg cctcaaccaa aatatcatca
     3361 gataaacttc ctgtcagaga taattgatga acagggaatt tcattgattg gtaatatgaa
     3421 aacatttttt cgctttcaac agcaattttg gtaacaaagg aattattggg aagccaagga
     3481 tgttctggag ctaaccccat cagttctaaa aagaaaattt gtttaccagt taacctgagc
     3541 atcttttttc ctttataaaa attaatccaa ttaggataga gtaaagatgc aagatagtta
     3601 gttatacttg aagatacatc aaaaatatcc ttgtcatata agctctccac tatttcctta
     3661 gttgtaccta gcgcaaaagg tatgattaat gtaggagtat ttcgtttatt acatagttta
     3721 attagcaagg aatttatcga gccaggaaac tcttgaccaa aaatcaaaag atcgtagtgg
     3781 tgcaacttaa gaactctata agcataacca aagtttttta gtacgccact tgtaatttga
     3841 aataaagaat tattttttaa taatcctcca aagaattttg aaatattaaa gaattttgaa
     3901 atattactga aaatcctttt taaaatcttg ctaaaaatct taatggcaac aataaaaata
     3961 caaagaaaac caattaaaaa caaaattgga taaaataaaa aagacgatct tctttttgat
     4021 ttgagtaaaa atgaatatgg tatatatccg ttatctagct gactttctac aggataactt
     4081 tttgaagatg aagattcatg cccacttttt gatttgatat taagagatt
//
LOCUS       DATNSN010000212         4124 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.213, whole genome shotgun sequence.
ACCESSION   DATNSN010000212 DATNSN010000000
VERSION     DATNSN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.213"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..189
                     /locus_tag="VAH76_002888"
     CDS             <1..189
                     /locus_tag="VAH76_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387468.1"
                     /translation="GYGRLKKVIALILSVGPIFILASYTISSKADNVLQADEQLATGE
                     HQKHKGHGGYGGGGVGGR"
     gene            337..795
                     /locus_tag="VAH76_002889"
     CDS             337..795
                     /locus_tag="VAH76_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387469.1"
                     /translation="MEHSWYDNNSKLSVMGIICLIIFLVLFFFSLFIMPFLIWELHYN
                     VPDFVSNMIAYLEDTYYYSSPVSKILVWLTFFIPCLITGYFSYYISRSIDKKNLLKDA
                     NIDEEQSKEISSVTNEQIKESASLGFKILILMAAIILIIFLLQEFVKFTS"
     gene            918..1520
                     /locus_tag="VAH76_002890"
     CDS             918..1520
                     /locus_tag="VAH76_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEO1387470.1"
                     /translation="MKKSMERSSDTLLEIASHQELKGEVTYQRILQVLGERAFGIVLL
                     FFALPSALPFSFIPGISLIFSVPILLFAFQMVFARKTMWLPKIIAERTIHQETIAKFI
                     HNTVPYLIKVEYFLKPRWLFMTSRFMEIIHGIVIFFLALLLMLPIPFSNFIFATLLII
                     FSLGLIEKDGLFLIIGYLGVVVYANFVYLFVLTAVKHLFG"
     gene            2020..3996
                     /locus_tag="VAH76_002891"
     CDS             2020..3996
                     /locus_tag="VAH76_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387471.1"
                     /translation="MTASVSFGETSNTMAKKLIHKTVDIYKYPDSLDSNSGEVTIGDL
                     HGNAVKFLHFLLRHQVVKFKNEKEEKQLFNEFVNLYEKSDEIAICYLNMKPIELRIND
                     HTQKLQEAKNRLDELSNLAERSEVEEQELALLNGPIGMYGSLLYKEKILNNQIASLNE
                     EKDKLAALGIALKDIPVMLDAIIDQLEVVSNDTLVRLIGDELADRGSNDYITLKLLGF
                     LHDNNVKVNITISNHSNEFIRSYEALNYQRPTGAIEGKDQRSFGGLKLLIDNNVVSKD
                     EVTKLVDKAYKPTLKVIDYTLSNEGISLFSHAPVRFDRIKIIAERMNIVYNDSTKEAL
                     ATTIDKINTRFDQIVKNNQVHEFCATAGVKVGDMSLDEISRLPLVDIIWNRWDASKDT
                     DDARPSKINNYSVNYVHGHDPYQSKFEHVINLDTTSGKGMRSEQNEKVKAAHEVIANK
                     SEGWEDAQAYLDGINEYKVLDSNERGLEQKHSLNLINEEYRQSKLMSPGKRKGIMTGI
                     FGAIGLGVGAAIGAALVATGVFAPFGAGVLGVVALAAVSGVGAGIVSGSLGFGIAKAT
                     APTPVNSTVVIPKNTSANTQSKVHSDIMSNQKGDSLSVLRKLGGTAKHKMEEEKPNIV
                     NIKEIDKPVVVSNPVIVPVEKDDNTLDYNNSPGL"
BASE COUNT         1324 a          632 c          824 g         1344 t
ORIGIN      
        1 ggttatggaa gattgaagaa agttattgct ttaatattat cagtgggccc aatatttatt
       61 ttggcttctt atactatatc atcaaaggcg gataatgttt tacaggctga tgagcaattg
      121 gctacaggtg aacaccaaaa gcataaaggg catgggggat atggaggtgg tggtgttgga
      181 ggacgttaga cgcggccgga aataagttta tttgtcgtta agctattttt taatatctag
      241 cttaaaatat attcggttat atgatggatg ctgctgatgt cttaaaattt aaaaaagaat
      301 aattttagtt agctggtcat tttatggaag gagcctatgg agcatagttg gtatgataac
      361 aacagcaaat tatctgtaat gggaataatt tgtttgatta tttttttagt cctctttttc
      421 tttagtctgt ttataatgcc ttttttaatc tgggaattac attataatgt tcctgacttt
      481 gttagcaata tgatagcata ccttgaggat acctattatt attcttcgcc tgtaagtaag
      541 attcttgtct ggttgacttt ttttatacct tgcttgatta ccgggtattt ttcttattac
      601 atttctcgct ctattgataa gaaaaatttg ctgaaagacg caaacattga tgaagaacaa
      661 tcaaaagaaa tttcttcggt aactaatgaa cagattaagg aatcagcaag tttaggattt
      721 aaaattctta ttctaatggc ggctattatt ttaatcattt tcttattaca agaattcgtt
      781 aaattcacat cataacttga agtatctctg tgttttccgc ggagttagag aaagtgtgag
      841 ctttaaaaat tcttaaggta aaaattttcc tcaaatagtt tgtaaattga tgttatatat
      901 tttgacttgg attttttatg aaaaaaagca tggagcgctc ttctgatacc cttttagaga
      961 tcgcgagtca tcaggagtta aagggggaag tcacttatca acgtattttg caagtgcttg
     1021 gagagcgcgc ctttggaatt gtgcttttat tttttgcatt gcccagtgcg ctgcctttct
     1081 catttattcc tggtatctcc ctgattttta gtgttccgat attactattt gcatttcaaa
     1141 tggtttttgc cagaaaaaca atgtggctac caaaaattat tgcggaacga accattcacc
     1201 aggaaacaat agccaagttt atacataata ccgttcctta cttaattaaa gttgaatatt
     1261 ttttaaaacc aagatggttg tttatgactt cccgctttat ggaaataatc catgggattg
     1321 ttattttctt tcttgcccta ttattgatgt taccaattcc atttagtaat tttatttttg
     1381 ctacgttgtt aattattttt agcttgggat taatagaaaa agatggatta tttctcatta
     1441 ttgggtatct aggggttgtg gtttatgcaa actttgtcta tctgtttgtc ttgacggcgg
     1501 tgaagcattt gtttggttga ttgaaagctt gtgttactaa ccattagagt tcactttttt
     1561 cattatagtt gaattaacca tatgtaaata aataaaaaat tccactttaa tcactcggaa
     1621 taactttgat gcagtaattt ttcaggttct cacataaccg aattaagatg ccttactatc
     1681 ctggagtctc agcatataat ggaatgcctg gctaacatca agtattaagc atgatacaca
     1741 cggaataaga tgatatggtt agatggtctt tgttttttgc agcaaatcga aatagtaaac
     1801 cccataaaat agttgaggaa gcttatgttt tcgttggtag ttcctttttc tttcaatgca
     1861 accatattta gctgaacgca agcgtacgat taatcattaa tctattgaaa aacaatagat
     1921 attattaaaa ataatatgac ttatgaaaac aatgagttgt acataaaatg attgacatga
     1981 ttaaatgtat gcattgggtg gttttacgag gaaataatca tgactgcgtc cgtgtctttt
     2041 ggtgaaactt caaacaccat ggcaaaaaaa cttattcata aaacagttga tatctataag
     2101 tatcctgact ccttggatag taattcagga gaagtaacta tcggtgattt gcatggaaat
     2161 gctgttaaat ttcttcactt tttactacgc catcaagtag taaaattcaa gaatgaaaaa
     2221 gaagaaaaac aattatttaa tgagtttgta aacctctacg aaaaaagtga tgagatagca
     2281 atttgttact tgaacatgaa acccatagaa ttgcgtataa atgatcacac ccagaaattg
     2341 caagaagcga aaaacaggct ggatgaactt agtaatctag ctgaaagaag cgaagtagaa
     2401 gaacaggaat tagctttatt aaatggtcca attggcatgt atggaagtct attatataag
     2461 gaaaaaattt taaacaacca gatagcaagc ttaaatgaag aaaaagacaa attagcggca
     2521 ttaggaattg ctctaaaaga cattcccgta atgcttgatg ctattattga tcagcttgaa
     2581 gtggtatcta atgatacgtt agttcggctg attggtgatg agttggctga cagaggcagc
     2641 aacgattaca ttacattaaa gcttcttggt tttctccatg acaataatgt caaggttaac
     2701 atcactatat caaaccatag caatgagttt atccgatcct atgaagcatt aaattaccag
     2761 cggccaactg gtgctattga aggcaaagat caacgatcat ttggtggttt aaagttattg
     2821 attgacaata acgtcgtttc caaagatgaa gtaactaaat tagtcgacaa agcatacaaa
     2881 ccaacactta aagtgatcga ttacacatta agcaatgaag gaatttcttt attttcacat
     2941 gctcctgtcc gatttgaccg cattaaaata atagcggagc gcatgaacat tgtttataac
     3001 gattccacta aagaagcttt agctacgact atcgataaaa taaatactcg ttttgaccaa
     3061 attgttaaaa ataatcaagt gcatgaattt tgtgctactg ctggggtaaa ggtaggtgat
     3121 atgtctcttg acgaaatatc aaggcttcca ttagtagata ttatttggaa tcgctgggat
     3181 gcatccaaag ataccgacga cgccagacca agtaagataa ataattattc tgttaactat
     3241 gtgcatggcc atgatcctta tcaaagtaaa tttgaacatg ttatcaatct tgataccact
     3301 tcaggtaaag gaatgcgatc tgaacaaaat gaaaaagtga aggctgcaca tgaagtaatc
     3361 gcaaataaat cagaaggttg ggaagatgcg caggcttatc ttgatgggat aaatgaatat
     3421 aaggtattgg attctaatga acgcgggcta gaacagaagc atagccttaa cctcattaat
     3481 gaggaatatc gtcaatctaa actgatgtcg ccaggtaagc gtaaaggaat catgacaggc
     3541 atttttggag cgattggttt aggtgttggc gcagcaattg gagctgcttt ggtggccaca
     3601 ggtgtatttg caccgtttgg cgcgggagta ttaggtgtgg ttgcgctagc cgctgtgtca
     3661 ggtgtgggcg cggggattgt ttcaggttca ctgggttttg gtattgcgaa agccacggca
     3721 cctacgccag ttaatagtac cgttgtgata cctaaaaaca catcggctaa tactcaaagc
     3781 aaagtgcatt cagacataat gagcaatcag aaaggcgata gtctcagtgt attacgtaaa
     3841 ttgggaggaa ccgctaagca taaaatggaa gaagaaaaac ccaatatagt taatattaaa
     3901 gagatagaca agcctgtggt tgtatccaat cccgtcatcg tgcctgttga gaaagatgac
     3961 aataccctgg attacaataa cagccctggt ttgtaatacg gcgtttggct ttgtttggcc
     4021 cgtggggagt aacggaacag ccaaccgact taagctatta aattgcttta atttttatcc
     4081 aaaatgattt atataagggt ttattaacaa taattttatc tctt
//
LOCUS       DATNSN010000213         4100 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.214, whole genome shotgun sequence.
ACCESSION   DATNSN010000213 DATNSN010000000
VERSION     DATNSN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.214"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(252..593)
                     /locus_tag="VAH76_002892"
     CDS             complement(252..593)
                     /locus_tag="VAH76_002892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387472.1"
                     /translation="MEIIVNLLIAAVSIICTWWISHCYYKKSLSDQTDHWLKIESTIW
                     GQTNNTNQENKLVLYEQRVRDAIQDYKIKGTPKYFIDTYNDLTQEEKAQMYDDVLLRA
                     KGRKGKNNPYN"
     gene            1184..2086
                     /locus_tag="VAH76_002893"
     CDS             1184..2086
                     /locus_tag="VAH76_002893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387473.1"
                     /translation="MKKYLLDNIYLLSDSIEPLSIRINKYCIHTHPVVSMNRRNYKRH
                     NKTASIEFEGSQEDSILYSGGRYGTKYKTRKRKLIEDILMIGSILTGWNWGLTSRRKY
                     NYYPLQPISNLSQLHISETCEVESHFKSALNRLLDKNWQIQYENGFHLRMLFNQANIN
                     HMESRFLSNMIIWEWLYPHEKNPNGATPNDESQDLKEIISYILTKHWPAQFKRQNQNN
                     IFHALRNQLAHSGTLPINRPHRYVDNWMKLIPWDDPNGSDLQSYLKFFNILTQVVVLK
                     TIDINADYLIQSQLNNYLSKGSID"
     gene            complement(2423..3238)
                     /locus_tag="VAH76_002894"
     CDS             complement(2423..3238)
                     /locus_tag="VAH76_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387474.1"
                     /translation="MILRMISLSFLFANAHELAALLELPYVQRLVYLMGIRPYMDRQS
                     CIVGIKRRISYQSLRETLYVGPIAGVKTGSPSLQQMKRAVKSLERAGLIEIQSTERHL
                     IVKCIFAELDNPAQKKAVPKPYLYPDTNPTQENALGSGESCQLRQKPDTDNTPEADPP
                     HKSEDYNYIYVCSQFEKFWKSYPHPVDREAAFYVFRELNPDDTLFGKIMAGLHRQIES
                     YNFQVMMGVWVPTWKYPANWLARKCWQTNPYVFEHSSPIVEDCDNQQVGIEED"
     gene            complement(3420..3704)
                     /locus_tag="VAH76_002895"
     CDS             complement(3420..3704)
                     /locus_tag="VAH76_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1387475.1"
                     /translation="MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLT
                     QEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHACGFDISMAVKRIAS"
     gene            complement(3701..4024)
                     /locus_tag="VAH76_002896"
     CDS             complement(3701..4024)
                     /locus_tag="VAH76_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO1387476.1"
                     /translation="MSWIVEFYNESVEEAILTMPPKIQARMIKLLELIETHGANLGPP
                     HTEAMGDGLFEIRAKAQEGIGRSLYCYMKGKHIVVLHAFVKKSAKTPKPDLQLALKRK
                     REVEK"
BASE COUNT         1312 a          783 c          654 g         1351 t
ORIGIN      
        1 aaattcaaga gcagttaaat actctatatt ctgacttgaa actgacagac ccattgactg
       61 tacaatcttt tgctaactca caagatgcta aaacgcaatc aaaatttcaa tataagataa
      121 ctaaaatcaa taaagatcta gacagcttga gtcaattact aaaaacacaa caaaatgaat
      181 tagatgctat taacaaatct ttaaataagc taacccctta attaatatac tgttcagata
      241 agctttcata atcaattata ggggttattc tttccttttc tacctttcgc ccttagtaaa
      301 acatcgtcat acatttgtgc tttttcttct tgtgttaaat cattataagt atcgatgaaa
      361 tatttagggg ttccttttat cttataatct tgaattgcat ctctcactct ttgttcataa
      421 aggaccaatt tattttcctg attagtatta ttagtttgac cccatattgt tgattctatt
      481 tttagccaat gatcagtctg atcagataaa gattttttat aataacaatg tgatatccac
      541 caagtgcata ttatagatac agctgctatt aacaaattaa caatgatctc catataaatt
      601 cccttttata tctattgatt acttaatttt gagctcatac agcagtgatc tatccctcat
      661 taaatcattt gccctgttct gagtttagtg ttggtcgagc ggctcactca aagttgaaac
      721 ccagcagtgc tgtattgttc gagttggggg gcaaatcaac cattatatgg taaacggtct
      781 cgcttattca cagctatttg ttaattggtt acctcctata tttttaaatt gatcaaacaa
      841 ttattaaaaa ttgttacctt gacttaccct actaaacgcc cagccacaag ttaagggtta
      901 aagtggcttt gcataagacg tcaatgacgt ttcgcctgtg cggctcatca gttatgcttc
      961 atttcaaaat agcaaataga taagtgctaa acaattattt ggagataaga attttgtcct
     1021 gacgccagac gggcgccagg acaaattcgc tagatgccct gattttacta agcaaaaggc
     1081 agggtatttt cctgcgtcac tgcacaggac ttgcagtgac gcagagataa acaatctata
     1141 cttaaaacaa taaattaaca aattccatgg atgatttatt aacatgaaaa aatacttact
     1201 ggacaacatt tatctacttt ctgattcgat agaaccgcta tcgataagaa taaacaaata
     1261 ttgcattcat actcaccctg tagtctctat gaatagacgc aattataaaa ggcataataa
     1321 aacagcatct attgaatttg aaggatcaca ggaagactcc atactttatt ctgggggtcg
     1381 ttatggcact aaatacaaaa caagaaaaag aaagttaata gaggatattc tcatgattgg
     1441 atctattctt acaggctgga attggggact aacttcaaga agaaaatata attattatcc
     1501 attgcaaccg atctcaaatt tatcccagct tcatatttct gaaacatgtg aagtagaatc
     1561 tcattttaaa agcgcactca ataggcttct cgataaaaat tggcaaattc aatatgaaaa
     1621 tggctttcac cttcgaatgt tatttaacca agcaaatata aaccatatgg aatcaagatt
     1681 cctttctaat atgattattt gggaatggtt atatccacat gaaaaaaatc ctaatggggc
     1741 gactcctaat gatgaatctc aggatttaaa agaaataatt agttatattt taacaaagca
     1801 ttggcctgca caatttaaaa ggcaaaatca gaataacatt ttccatgcat tacgtaatca
     1861 gttagcccac tcaggtacac taccaattaa tcggccgcat agatacgttg ataactggat
     1921 gaaactaata ccatgggatg atcccaatgg atcagattta caaagctact taaaattttt
     1981 caatatactc actcaagtgg ttgtgcttaa aaccatcgat attaatgctg attatttaat
     2041 tcagtcgcaa ctcaataact atttatcaaa aggctctatt gattaaaatg aaaatcctct
     2101 aagccaaaaa tccgtcttcc caatcatcaa actcttcttt aaaaataatg aaaaacggtt
     2161 acctaactaa agtgttaatc aaaaaatcat ttactctgaa ttgagttttg tgatgggtta
     2221 ataaggattt atcatataaa ttatgtccaa tcccgagcag cgtgatcgga caaatgagct
     2281 acgagtcttg attttactgg ttaaggttga attgttaatt ctgcgtcact gctggaaaat
     2341 ggcagtgacg cagatataaa tagagggaaa caataatttt tgactcttaa aaagatgggt
     2401 attatttgat atcccacgta cttcagtcct cttcaatacc tacctgttga ttatcacagt
     2461 cctctacaat tggtgatgaa tgctcaaaca catatggatt tgtttgccaa cattttctag
     2521 ctaaccagtt ggctggatat ttccatgtag gtacccatac tcccatcatg acctggaaat
     2581 tataactttc aatttgccta tgtagtcccg ccattatctt tccaaataag gtatcatcag
     2641 gattgagttc tcgaaataca taaaacgcag cttccctatc aacggggtgt ggataacttt
     2701 tccaaaattt ttcaaattgg ctacacacat atatataatt ataatcttct gacttatgag
     2761 gtgggtcggc ctcaggggta ttgtctgtgt ctggtttttg tcttaactga caggattccc
     2821 ctgatcctaa agcattttcc tgtgtcgggt ttgtgtcggg ataaaggtac ggcttcggta
     2881 cggccttctt ttgagcaggg ttatctaatt cggcgaaaat acactttact atcaagtgcc
     2941 tttcagtgga ctgaatctca ataagacctg cacgttcaag agatttaaca gcccgcttca
     3001 tttgctgaag gcttggactg cccgttttaa cgcctgcaat cggcccaaca tagagggttt
     3061 cacgtaacga ttgataactg atcctgcgct ttatgcctac aatgcaactt tgtctgtcca
     3121 tgtatggacg gatgcccatc aggtaaacta atctttgtac ataaggcaat tcaagtaacg
     3181 ccgctaattc atgggcattg gcgaataaaa aactcaagct aatcatcctt aaaatcatgc
     3241 ggtattctca ttttagatgt gtcatccgga tagtcaatat gattagaaat ggtgttacta
     3301 aatgatattg tttgaaccaa aaaataattt gagtttataa atattccaaa taatctagcc
     3361 atactagaat atttggaatt tattcaattt tgagaaatgt tatgaatatg aatcaaaaat
     3421 tatgaagcga ttctctttac tgccattgaa atatcaaacc cacacgcatg tgcatatttc
     3481 tctaaagtat tccagcttgg attagtattt cctttttcca gtcgagaaat attccctttt
     3541 tttgtcccca ttcgctcagc taactcttct tgagttaatc cagctttttg acgcatcgtc
     3601 agtaacgcat cgatcaatgc aaattcggat tccaaagcat cgtatgcttc ttttacttca
     3661 ggatttttta acgctttagc ctttaatgtt ttaagtttca tcatttttct acctcccgct
     3721 ttcttttgag agctaattgc aaatctggtt ttggagtttt tgcacttttc tttacaaatg
     3781 catgcaaaac cacaatatgc tttcccttca tatagcaata aagcgatcgc ccgattcctt
     3841 cttgagcttt tgcgcgaatc tcaaataacc catcacccat cgcttctgta tgaggaggcc
     3901 caagattagc gccatgtgtt tcaatcaatt ccaaaagttt aatcattcga gcctgaattt
     3961 ttggcggcat tgtaagtatg gcctcttcaa cagactcatt ataaaattca acaatccaag
     4021 acatttatta ttaaatcctt tgttatctta tatgataact tattgttatt attttttcca
     4081 gtcgcaaata aaaatgacaa
//
LOCUS       DATNSN010000214         4027 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.215, whole genome shotgun sequence.
ACCESSION   DATNSN010000214 DATNSN010000000
VERSION     DATNSN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.215"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            250..969
                     /locus_tag="VAH76_002897"
     CDS             250..969
                     /locus_tag="VAH76_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1387477.1"
                     /translation="MSTVAQYKNYYHYISDGWSKEPKEMFKFLAQKIKELPLKPGSKL
                     LDIGCATGELIHFLSKQFPQLQYTGIDVFDDLIEVCKELQPDVNFIKASVLDLPFEFE
                     SQFDIITVVSVISIFDTIDLDVFWENLFKATKDNGVIYVLSPFNEYGVDCEIRHRKRI
                     NNIKGEWERGWNIFCTETIAEYLEGRCKNWFFHPFKFNLELNQREDVIRTWTMKTEFN
                     DKQLTNGMKLMVDHYLLEIHI"
     gene            982..2073
                     /locus_tag="VAH76_002898"
     CDS             982..2073
                     /locus_tag="VAH76_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEO1387478.1"
                     /translation="MSQPFTIGPFSIGENHPPIFLAEIGTFFNQDLGIAEQLLHKIIT
                     IRDKVPYQPLILKTEILNNPNICLPVDYEETYTSKSGEVRKENYRSLIERKILPLEHY
                     SMLFSLLKNANLPFIVSIYELDTIDFAIKQGACALKTSSSNIVHIPLIRHLAKTGLPL
                     IIDTGRSSLTDICKAVETAKNAGCEKIIIEHSPDGHPALPEAHNLRMLRTLNQCFSLP
                     VGLSDHYNGIEMLYMSIALGASILEKGVHLYPEDIDQDISHTMGIDQLAKTLETIYNC
                     WQALGQPMRDLETPIKGVLGSSQRTCLVAKQKLQPGSLISLDNTTFAFPCKGVPTQYW
                     DLVNGWELQNKVSIGEPIKWSDIKQKNDC"
     gene            2063..3064
                     /locus_tag="VAH76_002899"
     CDS             2063..3064
                     /locus_tag="VAH76_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEO1387479.1"
                     /translation="MIVRNPTWNSLTPPLPKFVSCDTVETDFLKEKLGEDGGIYIAQF
                     DLKSTSAFLFQRKSDQKKFFIKKVSSEHKRQYQQSEHLAQFIACSDYIVNIAINCVSN
                     EEENSLYYIYPYIHGKRLFAEPEEIISLATALAKLHLKKKSYPDQQLIIKNTTERTSQ
                     LNFIRKALADGCYSYIPHFSFVKKMAQQYDFDWINQEDAQPIHGDLNAGNLLLSEDNM
                     ICFFDFEDALHSFHPVVLDLLFVIERIIFNQNSSTEQLLNLGLMFIHAYKKAGGTYQY
                     KTRDEFGLTILALKAFCLLTLLAEKNKNILSSEWDKFLKLTQKAENERGLIKTILQG"
     gene            3075..>4027
                     /locus_tag="VAH76_002900"
     CDS             3075..>4027
                     /locus_tag="VAH76_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387480.1"
                     /translation="MRILVTARDVGAALNIIEIVKILKQYAGVTVFIYAQPPASKYFL
                     RAGIQPVFEVPLPPSRSLNEASDLLSYANQLIDKLNPDIILSGLSSPGEAGIDEAIIA
                     ECPAHIKTFVLQDFWGEVNFFFEKLADCYLCIDYHAAEITKKRFNAKTEVIGSPRHAI
                     YQTLNIEEQRNYIREYFNITQSTKTIGLFGQALHGIPGYMETISQWLQACLQQEKTKL
                     VIFYRPHPRESEADKEETIRLLNSSGLEWKILSTKLVEESFLACDLVCSFFSNCLYDA
                     AYLNYFSLNPMVIPVVMNFHPEIKKYTNQLNLYEISPYRNQ"
BASE COUNT         1393 a          683 c          677 g         1274 t
ORIGIN      
        1 aatatttaat tcaatgcaat caaattgaaa tagattttct tctcctcaaa tagagcaatt
       61 atattttgat tatggtcctt aatccacaaa actaacatgc ttctaaatta atcctttact
      121 ttgtagtatt cataattttc attttgtgtt aaaacacccc aacagatgac ataggtcaaa
      181 atttctgtta aaaagcgcat aaacaaccta aaatacaata ttccttgagg gaaacggagg
      241 ttatgattta tgtcaactgt cgcgcaatac aagaactact atcattatat ttccgacggc
      301 tggagcaaag aacctaaaga aatgttcaaa tttcttgccc aaaaaattaa agagctgcca
      361 ttaaagcctg gaagcaaact attagatata ggatgtgcaa caggagaatt aattcatttt
      421 ttgagtaaac aattcccaca actgcagtat accggaatag atgtttttga tgacttgatt
      481 gaggtatgta aagaactaca accggatgta aattttatta aagcatcagt tttggatctg
      541 ccttttgaat ttgaaagtca atttgatatt attactgtag tgagtgttat ttctatcttt
      601 gatactatcg acttggatgt attctgggaa aatctcttta aagcaaccaa agataacgga
      661 gtaatctatg tcttgtcacc atttaatgaa tatggggtag attgtgaaat acgacataga
      721 aaaagaataa ataatattaa aggggaatgg gaacgcggtt ggaatatttt ttgcacagaa
      781 acgatagcag aatatcttga gggtcgttgt aaaaattggt tttttcatcc tttcaaattt
      841 aatcttgaat tgaatcagcg tgaagacgta attagaacct ggacaatgaa aaccgagttt
      901 aatgataagc aattaactaa tggtatgaaa ttaatggtag atcattattt attagaaatt
      961 cacatataaa cgagacagtc aatgagtcag ccttttacaa ttggaccatt ctctattgga
     1021 gaaaatcatc ctcctatttt tttagcagaa ataggtactt tttttaatca ggatcttggc
     1081 atagctgaac aattgctaca caaaattata accatcagag ataaagtacc gtatcaacct
     1141 cttatactaa aaacagaaat tttaaataac ccaaacattt gcctacctgt tgattatgaa
     1201 gaaacctata ctagtaaatc aggtgaggtt cgcaaggaaa actaccgctc attaattgag
     1261 agaaaaatac tgcctttaga acattacagt atgctattct cactattaaa gaatgccaat
     1321 ttacccttca tagtttcaat ttatgaattg gacactattg attttgcaat aaagcaaggg
     1381 gcctgcgcac tcaaaacctc ttcatcgaac attgtccata ttcctttaat tcgtcatctg
     1441 gcaaaaacag gattaccgct tattattgac actggaagat cttccttaac tgatatatgc
     1501 aaagcagttg aaacagcaaa aaatgccgga tgtgaaaaaa ttattattga acatagtcct
     1561 gatggacatc ctgctttgcc agaagcacat aacttaagaa tgttacgaac tttaaaccaa
     1621 tgtttttcat taccagttgg attatctgac cattataatg gaattgaaat gttgtatatg
     1681 tctattgcat tgggagcttc aatcctggaa aaaggggttc atctctatcc agaagatatt
     1741 gatcaggaca tatcacatac tatggggatt gatcaattag ccaagactct tgaaacaatt
     1801 tacaattgtt ggcaagcttt aggtcaaccg atgcgagatc tagaaacccc tataaaagga
     1861 gtacttggca gctctcaaag aacttgtttg gttgcaaagc aaaagcttca acccggctct
     1921 ctaattagcc ttgataatac aacctttgcc tttccttgta agggagttcc tacccaatac
     1981 tgggatctgg taaatggctg ggaactacaa aacaaggtat ctataggtga gcccattaaa
     2041 tggtcagata ttaagcaaaa aaatgattgt tagaaaccct acttggaact ctcttacccc
     2101 tccgcttcct aaatttgttt cttgtgatac agttgagaca gatttcctga aagaaaaatt
     2161 aggcgaagat ggtgggattt atattgctca atttgatcta aaatcaactt ctgctttttt
     2221 atttcagcgt aaaagtgatc aaaaaaaatt ttttatcaag aaagtatcct ctgaacataa
     2281 aaggcagtat cagcagagcg aacatttggc tcaatttatc gcttgctcag attatatagt
     2341 taatattgca attaactgcg tttctaatga agaagaaaac tcactctatt atatttatcc
     2401 ctatattcat ggaaaacgcc tctttgctga accagaggaa ataataagcc ttgcaactgc
     2461 gttagcaaaa ttacatctta aaaaaaaatc ttatcctgac caacaattga ttattaaaaa
     2521 taccacggaa cgaacatcac agcttaattt tataagaaag gctttagctg atggatgtta
     2581 ttcttacatt ccacatttct cttttgtcaa aaagatggct caacaatatg attttgattg
     2641 gattaatcaa gaagatgccc aaccaattca tggtgattta aatgcaggaa atctgttact
     2701 atccgaagat aatatgattt gcttttttga ctttgaagac gcattacata gtttccatcc
     2761 agtagtgtta gatttgctat tcgtcattga gcgcattatt tttaatcaaa atagttcgac
     2821 tgaacagcta ttaaaccttg gcctcatgtt tattcatgca tataaaaagg ctggtggcac
     2881 ataccagtat aaaacaagag atgaatttgg attaacaatt cttgctttaa aagcattttg
     2941 tttgttgact ttgctagcag agaaaaataa aaatatactg agttcggaat gggataaatt
     3001 tttaaaatta acccaaaaag cagaaaatga acggggtttg ataaaaacaa ttttacaagg
     3061 ataagaaaga tagtgtgaga attcttgtga ctgcgcggga tgttggtgct gcgctaaaca
     3121 ttattgagat agtaaaaata cttaagcaat atgcaggtgt tactgtattt atttatgctc
     3181 aaccccctgc ttcaaaatat tttcttcggg ctgggattca acctgtattt gaagttccac
     3241 taccaccaag ccgatcctta aatgaagcca gtgacctact atcctacgcc aatcaattaa
     3301 ttgataaatt aaatcccgat atcatattat ctggattatc atctcctggc gaagctggca
     3361 tagatgaagc aataatagct gagtgtcctg cgcatatcaa aacattcgtc cttcaagatt
     3421 tttggggaga agttaatttt ttctttgaaa aattagctga ttgctattta tgcatagatt
     3481 atcacgctgc agaaattaca aaaaaacgat ttaatgccaa aaccgaggtg ataggctccc
     3541 ctcgccatgc catttatcaa acattaaata ttgaggaaca acgcaactat ataagagaat
     3601 attttaacat tactcaaagt acaaaaacta ttggtctttt tgggcaagca cttcatggta
     3661 tacctggata tatggaaacc atttcacaat ggttgcaagc ttgcttacaa caagaaaaaa
     3721 ccaaattagt tattttttat cgcccccatc ccagagaatc agaagctgac aaagaagaaa
     3781 ccataagatt acttaattca tctggattag agtggaaaat tttgtcgact aaattagttg
     3841 aagaatcatt tttagcatgt gatttggttt gtagcttttt ttctaattgt ctttacgacg
     3901 cagcctattt aaattatttt tctttaaacc ctatggtaat tccagttgtg atgaattttc
     3961 acccggagat taaaaaatat acaaatcaat taaatttata tgaaatctct ccttatcgga
     4021 atcaaga
//
LOCUS       DATNSN010000215         4007 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.216, whole genome shotgun sequence.
ACCESSION   DATNSN010000215 DATNSN010000000
VERSION     DATNSN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.216"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            125..973
                     /locus_tag="VAH76_002901"
     CDS             125..973
                     /locus_tag="VAH76_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEO1387481.1"
                     /translation="MFELKRDLNSLPLTWLGRCVYFLSSYKRSNAKKNIDRVFQFALS
                     PAEKKRLCMAYYSHIITNIKEIILYALVSKKSLEKRIKVIGIEHLENALGKGKGILVF
                     TGHFGNWEFAPLFFLDKFNGKDLDFYCVRKNLRFAFLDSIFLRRFENCGFKIISHKNA
                     VSQTRRALKNRAIVLFPFDVCPSSKIKNKVKADFLGQKSETNMSLAYLAALCGSSVLS
                     VTFYRTNKKQHVVHIYPEIKPVVCPDYKQTLVENTQLYNKRLEEMLLPYPEQWLWSYK
                     RWKNNG"
     gene            966..1802
                     /locus_tag="VAH76_002902"
     CDS             966..1802
                     /locus_tag="VAH76_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEO1387482.1"
                     /translation="MANYHLSKLRVTFAGLLFYWVLPLRKQIVLSNIDLVFKNTATSA
                     EKTRLAKAFYSHVASILKELFWMGWASSKRLTNKIEIHGLEHLESAVNQDKGVFLLTG
                     HLGNWELAYTLGFSRLMPQYGQFNIIRRPIKIKWLESILFNRMMRYGLTIISSVSAPK
                     KINQALRNKELVLFTLDQHAKLENKSGIAVEFFGLHAGTYRSLAFFAYKHQTPVVPVS
                     GYRQHNGKHVIKFYPEIEWEYHADKEQAIYQNTLRYNQALEQLILEHPEQWWWVHRRW
                     KL"
     gene            complement(2072..3217)
                     /locus_tag="VAH76_002903"
     CDS             complement(2072..3217)
                     /locus_tag="VAH76_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387483.1"
                     /translation="MITSIQDIARHIVRAGKSMVWFEFDSRKDAETALNLIKKQYSDL
                     HNLHKASVNSGNVNKHKFGFGLTHTQFKSITKFNENSQISATGLKVQASISQTNQKQS
                     PEALDKSTNRLVISTQSLDQENIALENPNTADLSMNHSNLEKVGNGHSALAIASALNS
                     PLVVLRSEQNQEKILKTVGKIGNNSLVFITGHCGKGSNSLGGLYFSDSKESMINVSRT
                     PDEIVHFLASAGLKRGDNITIVLCVCYGAKEGEYLQHHSFASRLAISLAEIGVSSTIY
                     ASKGRTTRFGKEAIVNGAITFNSGLGIKPNELVVFETMIRDSLSTVKTSQRELKAAIS
                     ISGEGLQIKHPETSPSSNNSLVGFFKPSMTIDSNPQSGNWAQRNDLK"
     gene            3690..3905
                     /locus_tag="VAH76_002904"
     CDS             3690..3905
                     /locus_tag="VAH76_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387484.1"
                     /translation="MVKKYPILIIGGYGNFGKYIARVLSRDLRIALSIFGRDRQKAED
                     LVNSIKSANPITIHSCDIFQNPAETLK"
BASE COUNT         1232 a          716 c          728 g         1331 t
ORIGIN      
        1 aggagtcagt cagtatcagt aagttctgtt tactttaaaa tgattagatt gtagactgcc
       61 tgtctaaacc aggcttcata gtcaggaata gtgcttaggc aaaacacaag gtagttttct
      121 ttttatgttc gagctgaaac gtgatttaaa ttctcttccc ttgacctggt tgggacgctg
      181 cgtctatttt ttatcttctt acaaaagaag taatgcaaaa aaaaatatcg atcgtgtttt
      241 tcaattcgcc ctatcacctg ctgaaaaaaa acgtttatgc atggcttatt attctcatat
      301 tataaccaat atcaaagaaa ttattttata cgcgttggtc agtaaaaaat ctttggaaaa
      361 gcgtatcaaa gtcatcggta ttgagcattt agagaacgct ttaggtaagg gaaaaggaat
      421 tttagtgttc actggacact ttggtaactg ggagtttgca cccttattct ttcttgacaa
      481 atttaatggc aaggatcttg atttttattg tgttcgcaaa aatttgcgct ttgctttttt
      541 ggattccatt tttttgcggc gatttgaaaa ttgcggattt aagattatta gtcataaaaa
      601 tgcggtgagt cagacccgca gggctttgaa aaacagggcg attgtacttt ttccttttga
      661 tgtttgccct tcttccaaaa ttaaaaataa agtgaaggct gattttttag gtcagaaatc
      721 ggagaccaat atgagtttgg cttatttggc tgctctgtgt ggctccagtg tgttgtccgt
      781 cactttttat cgcaccaata aaaagcaaca tgtagtgcat atttatccgg agataaaacc
      841 ggttgtttgt ccggactata aacagacact tgtcgaaaac actcagcttt ataataaaag
      901 attggaagag atgttattgc cttatcctga gcaatggcta tggtcataca aacgttggaa
      961 aaataatggc taactatcat ttgtccaaac tgcgagtgac ttttgcaggg cttctttttt
     1021 actgggtttt acctcttcgc aagcaaattg tattgagtaa tatcgacctc gtttttaaaa
     1081 atacagcgac ttctgctgaa aaaacaagat tggccaaagc gttttactct catgtcgcat
     1141 ctattttgaa agaactcttt tggatgggct gggcaagctc taaaagactg actaataaga
     1201 tagagattca tggtttagag catttggaaa gcgctgtgaa tcaggataaa ggggtttttc
     1261 tgctcactgg tcatttaggt aattgggaac tagcctacac tttaggtttt tctcgcctga
     1321 tgcctcaata tgggcaattt aatatcatca gacgacccat taaaataaaa tggctggaat
     1381 caatattgtt taataggatg atgcgctatg gattgacaat tatcagtagc gttagtgcgc
     1441 caaaaaaaat aaatcaagcc ttaagaaaca aggaattagt cctttttaca ctcgatcagc
     1501 atgcgaaatt ggaaaacaaa tcagggattg ccgtagagtt ttttggcctt catgctggaa
     1561 cataccgaag tttggctttt tttgcctata aacatcaaac accagtagtc cccgtatccg
     1621 gttataggca acacaacggc aaacacgtta ttaaatttta tccagagata gaatgggaat
     1681 atcatgcaga caaagaacaa gctatctatc aaaataccct gcgctataat caagcgcttg
     1741 aacaattaat tttagagcac ccggagcaat ggtggtgggt tcatagacga tggaaattat
     1801 agccttatag attttgactt atcgatagta aatccccatt aatactacaa tagagatata
     1861 atttttcctg cactgagaat ttgtcataat ggacttaggc gcactaacaa attctttcat
     1921 aattttttta cgctcattag tgctctgctt tccatccaca ttaagtatta tattcaatac
     1981 aaattgatta tttaatggct tttttaaatc gctttgcgaa acaatttgga gtcaaagagc
     2041 aagagcccct tgactattag atagttgtaa attacttcaa atcgtttctt tgagcccaat
     2101 ttccagattg cggattcgaa tcaattgtca ttgagggttt aaagaatcct acaagtgagt
     2161 tgttagaact tggagaagtc tcaggatgtt ttatttgtaa tccttcacct gaaatcgaaa
     2221 tagctgcctt taattctctt tgactggttt taacagttga taaactatcc cttatcatgg
     2281 tttcaaaaac caccaattca tttggtttta ttcctaaacc tgaattaaag gtaatggcac
     2341 cattgacaat ggcttccttt ccaaaccgtg ttgttcttcc tttagaagcg taaatagttg
     2401 aactcacccc aatctctgca agactgattg caagtctaga agcaaaactg tgatgctgta
     2461 gatactcacc ttcttttgca ccataacata cacataacac tatcgtgatg ttatctcctc
     2521 tttttaatcc agcactagct aaaaaatgaa caatttcatc gggagttcga ctcacgttta
     2581 tcattgattc ttttgaatca ctaaagtata gaccaccaag ggaattagaa cctttaccac
     2641 aatgacccgt tataaatacc aggctattat tacctatctt accaaccgtc ttaaggatct
     2701 tttcttgatt ttgttctgaa cgtaatacca ctagaggact atttaaagca ctcgctattg
     2761 ctaaagcaga gtgtccattt ccaacttttt ctaaattact atggttcatc gacaggtcag
     2821 ctgtattcgg attttctaat gctatatttt cttgatcgag gctttgagtg ctaataacaa
     2881 gtcgattagt ggatttatcc agagcttccg gtgattgttt ttgatttgtt tgtgatattg
     2941 aagcttgaac ttttaatcca gtagctgata tttgggagtt ttcgttaaac ttagtaatac
     3001 tcttgaactg cgtatgagta agaccgaacc cgaatttatg cttatttaca tttccagaat
     3061 ttaccgatgc tttatgcaaa ttatgtaagt cactgtattg ttttttaatt aaattcagtg
     3121 ccgtttcagc atcctttctc gaatcaaact caaaccacac catgctcttt cctgctctca
     3181 ctatatgacg tgctatatct tgtatgcttg taatcatttt cctatctccg taaagatgtg
     3241 ttggtttcaa ctcaatccgg ggcttgatta aaaacgttct tttcctattt gctgttcagt
     3301 gtttaacctg aaataggcaa cgtgaaatat caaaccttct taagatggaa ttgaaaataa
     3361 attaataagg tgtttgcgtg ctttatttaa aattcaaata agcgcttatt attggagata
     3421 ggcatcagga ataattttat ggctcggaaa acgagctact acgtggaaac taataagaat
     3481 attagactta aaggttagtt gcataattaa atcatcaact ttaaattgat aacaataata
     3541 tcatggtgat tattttttgc gaatactttc gcttggcggt ctctgaggca ctgtagatat
     3601 taactaacga atcgcgcata ttattcatcc ctaaacaaaa caccctaggt agggatatgg
     3661 ataatctctc acaaggaatg tagtctttaa tggttaaaaa gtatcctatt cttataattg
     3721 gtggttatgg taatttcggt aaatacattg ccagagtact cagccgagac ctacgaattg
     3781 ccctgtcaat ttttggtcga gatcgacaaa aagcagagga tttagtaaac agtataaaat
     3841 cagctaatcc tataaccatt cacagctgtg atatttttca aaacccagcg gaaactctta
     3901 agtaagttaa cccctataag gaacatttcg gcccaattga aggtcaaata tagcctgttt
     3961 gtaatagata agatcaaaaa gtataattat aagagagcat caaacta
//
LOCUS       DATNSN010000216         3875 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.217, whole genome shotgun sequence.
ACCESSION   DATNSN010000216 DATNSN010000000
VERSION     DATNSN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.217"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..822
                     /gene="legL1"
                     /locus_tag="VAH76_002905"
     CDS             <1..822
                     /gene="legL1"
                     /locus_tag="VAH76_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL1"
                     /protein_id="HEO1387485.1"
                     /translation="LNLRDNDIPLLCEFLQNHPAITSLDLSHNDITANGMKLFVNKTS
                     VSSLNISHNNIGPEGAQWLSEDNHITTLDVSFNEIGDEGIKALAANAKLITLYALYNK
                     ITKVGAGYLAQSNLKKIDLCFNSLEDEGVIALASNINIKELIASACDVSDIGAIELAK
                     NNQLTLLILGKNAITDKSTLHFANNTSLSTLHLGSNQITAAGKKILETNTRITDLDLI
                     GNPIEMHETDELNYSKKSANSHSVFKFLQKFTFLSCMSPCQETAESDKGLNKKPN"
     gene            984..2159
                     /gene="ravJ"
                     /locus_tag="VAH76_002906"
     CDS             984..2159
                     /gene="ravJ"
                     /locus_tag="VAH76_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavJ"
                     /protein_id="HEO1387486.1"
                     /translation="MAIAPQQIQERLKQEQYQKFVVADIGNFPHCLAQTPEGIASGQR
                     YQKYSTNSLSRTPPFSQWGAPQLLTPKSAQEYIKFAQQRNKKSSFKIDGEAVRVSECS
                     NFAYHAAGVLLDDPQIRTQYDVAVIGSMHSNGRYLHNITLLVPKGSRLPQPPQQLTAE
                     VFPTGTLIVDPWAVGMGHPPEQALAIPKEQFAYNRSLFPATVNYQSALDESLTSARTG
                     QLTPYTGTPSQTGRQEVRPESQFPDTRRSVSATFPGNFAAYHELWRNAFQEIMNDPRH
                     QLHRNDVEYKKIHAIRTVLDDYTKGGDTWWAKFRRIFTFHWNRHHVKVVDDIVKEIDA
                     GNYTTSRALVDRLDNLAISLGSKLNPKGTLKEQIGFIKIQEQNPVEEIENLSRPHLT"
     gene            complement(2215..2904)
                     /gene="recO"
                     /locus_tag="VAH76_002907"
     CDS             complement(2215..2904)
                     /gene="recO"
                     /locus_tag="VAH76_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO1387487.1"
                     /translation="MTSKSLNAWVIHKQWSGDTSARLKLFTKELGLINCLCKGGRTPK
                     KQSLLQAFIPLWVSIEERYDQYYTRNIESTSSRLDLEGHSLFSGLYINELLYYTLSPD
                     FPDPDLFDAYLFTLNGIALAREREAIEALLRRFEWALLKACGYTFSFLHEARTGELIV
                     PDSHYQFVAGEGFILGGDKKIPGEHLLAIAADNLSESAYLKSAKFIMRQAIDHLLGGR
                     EIKARSLYGPA"
     gene            complement(2894..3781)
                     /gene="era"
                     /locus_tag="VAH76_002908"
     CDS             complement(2894..3781)
                     /gene="era"
                     /locus_tag="VAH76_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO1387488.1"
                     /translation="MSSYCGYIALVGRPNVGKSTLLNRILQQKLSITSRKPQTTRHSI
                     LGIRTEDEFQFVYVDTPGIHQGNAKAINRMMNKTAISVLRDVDVIAFLVDGTHWKDED
                     EYVLNLIKHAKVPCILVVNKVDKITDKTQLLPWIEQISQRYQFAAIIPLSAKTGLQVD
                     ELEGKLKTYLPEGPHLFPDDQFTDRSTKFLCAELLREKIFRFCGQELPYSVTVDIESF
                     KDEGNLIRIHALILVDKDNHKRMIIGDKGQKLKEMATNARLDMEKMLDKKVFLQCWCK
                     VKSGWSDDERILKQLGYDQ"
BASE COUNT         1261 a          824 c          667 g         1123 t
ORIGIN      
        1 ttaaatctta gagataatga cataccactg ctatgtgagt ttcttcaaaa tcatcctgcc
       61 ataaccagtc ttgacctgtc acataatgat attacagcca atggtatgaa gctttttgtt
      121 aataaaacat cggtttcatc ccttaacatt agccataata atattggtcc ggagggtgct
      181 caatggctat cagaagataa ccacattact acgttagatg tcagttttaa cgagattggt
      241 gacgagggaa ttaaagctct agccgctaac gccaaactga taacccttta tgcactttat
      301 aataagataa ccaaagtagg tgcaggctat cttgctcaat ccaatcttaa gaaaatcgat
      361 ctctgcttca atagtctgga agatgagggg gttatagcat tagcaagtaa tatcaatata
      421 aaagagctga ttgcaagcgc ttgtgatgtc tctgatattg gagctattga attagctaaa
      481 aacaatcaac tgactttact tattttggga aaaaacgcga taaccgataa atcaacactc
      541 cattttgcta ataacacgtc tttgagtacc cttcatctgg gtagtaatca aattacagca
      601 gcaggtaaaa aaatacttga aaccaatacc aggataacgg acctcgattt aatcggcaat
      661 ccaattgaaa tgcatgaaac agatgaacta aattattcta aaaaatcggc aaactcccat
      721 agtgtattta agtttttgca aaaatttact tttctcagtt gtatgtcacc ttgtcaagaa
      781 accgcagaaa gcgataaagg cttaaataaa aaaccgaatt aattatgaaa tcacaattat
      841 cataatcaag attcagtaaa aacaacaacc caagaggtaa ttttttattc atatccacaa
      901 aaataaaagc actatatttt taaagaaatc atggttaaga aataaccgcc acaataaact
      961 caaatcagtt atcggagtaa tttatggcta ttgcccccca acagatacaa gagagactga
     1021 agcaagaaca atatcaaaaa tttgttgttg ctgatattgg gaattttcca cattgccttg
     1081 ctcagactcc tgaaggcata gctagcggac aaagatacca aaaatacagt acaaactcct
     1141 tatccagaac accacctttt agtcaatggg gagctcccca gctcttaacc cctaaatcgg
     1201 ctcaggaata tattaaattt gcccagcaaa gaaataaaaa atcaagtttt aaaattgatg
     1261 gcgaggcagt tcgtgtctct gaatgcagca attttgctta ccacgctgca ggagtattgt
     1321 tagatgatcc acaaataaga actcagtatg atgttgccgt tataggaagt atgcacagta
     1381 acggccggta tctacataat attaccttac tcgttcctaa aggaagcaga ctccctcaac
     1441 ccccacagca attaaccgct gaagtttttc caactggtac gctaatcgtt gatccctggg
     1501 ccgttggcat gggacaccct cctgaacaag cattggcgat tcccaaagaa caatttgctt
     1561 ataatcgcag cctttttcct gccacagtga attatcagtc agctcttgac gagagtctta
     1621 cttcagccag aacaggccag ctcactccct atactggtac accttcacaa actggaagac
     1681 aagaagtacg cccagaaagc caattccctg atacaagaag gtctgtttct gcaacgttcc
     1741 ccggaaattt tgctgcttat catgaattat ggcgaaatgc ttttcaagaa attatgaatg
     1801 atcctcgcca tcagctccac agaaatgatg tagaatacaa aaagatacat gccattagaa
     1861 ctgttcttga tgattatact aaaggaggcg acacctggtg ggccaaattc agacgaattt
     1921 tcacttttca ttggaataga caccatgtca aagtagttga tgacattgtc aaagagattg
     1981 atgctggtaa ttacactacc tcacgagctt tggttgatag attagacaat cttgctattt
     2041 cattgggaag taaactcaat cctaaaggta ctttaaaaga gcaaattggt tttattaaaa
     2101 ttcaggagca aaatcctgtg gaagaaatcg aaaatctatc cagacctcac ctaacataat
     2161 aaaccctgga cctgatgtga tagcatagaa gcgcttggcg atcataccac tatctcaggc
     2221 cggtccatac aaggaccggg ccttgatttc ccgtcccccc aaaagatgat cgattgcctg
     2281 acgcataatg aatttcgctg attttaaata agcggactcg cttaaattat ctgccgctat
     2341 agcaagtaaa tgctcaccag gtattttttt atcaccacct aaaataaagc cctccccagc
     2401 tacaaattgg taatgagaat cgggaacaat taactctcct gttcgcgcct catgaagaaa
     2461 tgaaaatgtg tatccacaag ccttaagcaa agcccattca aagcgacgca gcaaagcttc
     2521 tatcgcctct ctttctctag ctaatgctat gccatttaag gtgaacaagt aagcatcaaa
     2581 taaatccggg tcagggaaat ccgggctcag cgtataatag agtaactcat tgatgtacag
     2641 gcctgaaaaa agagaatgtc cttcaagatc cagtctggaa gaagtgcttt caatgtttcg
     2701 tgtgtaatat tgatcataac gctcttcgat actaacccac agaggaataa aagcttgaag
     2761 taatgactgt ttcttgggag ttcgccctcc cttgcacaag caattaatta atcccagttc
     2821 cttggtaaaa agctttagtc tggcactggt atctccagac cattgcttat gaattaccca
     2881 agcattcagc gatttactgg tcatatccca attgcttcag tattcgctca tcatctgacc
     2941 agcctgattt cactttacac caacattgta aaaaaacctt tttatctagc attttttcca
     3001 tgtccaaacg ggcattagtt gccatttctt ttaatttttg gcctttatca ccaatgatca
     3061 tgcgcttgtg attatccttg tctactaaaa tcaaggcatg aatacgaatt aaatttccct
     3121 catccttaaa tgattcgata tctactgtta ctgaataagg caattcttgt ccacaaaagc
     3181 ggaaaatttt ttcacggagc aattcagcac ataaaaattt tgtagaccga tcggtgaatt
     3241 gatcatcagg gaacaaatgt ggtccttctg gcaaataagt ctttaatttg ccttccaatt
     3301 catccacctg caaaccagtt ttcgccgaca aaggaataat ggctgcaaat tgatatcgct
     3361 gacttatttg ctctatccaa ggcaaaagtt gtgttttatc agttatcttg tcaactttgt
     3421 taactaccag gatgcaagga actttagcgt gtttgattaa attcagcaca tactcatctt
     3481 cgtctttcca atgcgttcca tcaactagaa aggcaatcac atccacgtca cgtagtacac
     3541 taatcgctgt tttattcatc atgcgattaa ttgctttcgc attaccctga tgaataccag
     3601 gagtatctac ataaacaaac tgaaattcat cttctgtacg gattcctaaa atactatggc
     3661 gcgtggtttg tggttttctc gaggtaatac ttaatttttg ctgtaaaata cgattcaata
     3721 aggttgattt gccaacattt ggcctgccaa ctaaagcaat ataaccacaa taactactca
     3781 ttaatcttga ttcctgttta tttttaaaac attctaacag ccatgtcata aaacttccag
     3841 agataccaaa tttttcatta aataggcttg ctatt
//
LOCUS       DATNSN010000217         3867 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.218, whole genome shotgun sequence.
ACCESSION   DATNSN010000217 DATNSN010000000
VERSION     DATNSN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.218"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..357)
                     /gene="lem24"
                     /locus_tag="VAH76_002909"
     CDS             complement(<1..357)
                     /gene="lem24"
                     /locus_tag="VAH76_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem24"
                     /protein_id="HEO1387489.1"
                     /translation="MWIFKDITDRYYKKILDSYQLQADEKTYKGKEAELLIQRNELAR
                     CLIKTISLMEVKTQEDYANYFKRVSDEINSALQAAKNAVKEYNEEHNTDFTTDLYESF
                     FTYSLSNFIEVVSDLFQ"
     gene            887..1966
                     /locus_tag="VAH76_002910"
     CDS             887..1966
                     /locus_tag="VAH76_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="HEO1387490.1"
                     /translation="MKSKYISLCFFSLILWFGSPILLAQPSRLDNLINQLVQPFIYAH
                     QTPGLAIAIYYNGKDYYYNFGFADRKSKKPVTKNTIFELASITKIFISTLVALEVQKG
                     KLNVTDSAVQYTPELSKTNGLPIDRVQIVHLATHTASFPKQMEQFGVNKGNIQAFFIR
                     LKTWRPPVKIGTHYKYSNIGFGYLGYVLENASNETLPNLLKSNITKPLGMSHTFFNVP
                     KSLANYEAQGYRPKNKPAPYYTPANFLGGGALRSSSADMLVFLKANLGIQPGTASPEL
                     LSAMQYAQQPFFEVNPNFVMGLGWQRVKRGRHIVITKNGANQGFSTFIGFSPAKKLGV
                     VVLINQARGKATALGNQILNSLLDL"
     gene            2217..2654
                     /locus_tag="VAH76_002911"
     CDS             2217..2654
                     /locus_tag="VAH76_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387491.1"
                     /translation="MKKSNHVHSLLLLTAITFSPVIKASPVGLIVDIYCPTTQGGPNV
                     ITNFGDYIAGYGMENILSQNNPIYFKSISLAHDVPAQLGNYYNETTSYNSTTGQVTCS
                     YISNNPTEPRFAVAYTLTNGRGGSVQWQSGNSINIIFPVGLKS"
     gene            complement(2670..2819)
                     /locus_tag="VAH76_002912"
     CDS             complement(2670..2819)
                     /locus_tag="VAH76_002912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387492.1"
                     /translation="MRFSLNTPIIRYVLLLGLALALIAEFLSISPANQFQKELLGDEE
                     DIQAF"
     gene            complement(2873..3166)
                     /locus_tag="VAH76_002913"
     CDS             complement(2873..3166)
                     /locus_tag="VAH76_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387493.1"
                     /translation="MNVNSENAQPGEAVTAEDYSRAMNFIGQNLLSTLTQSVEKLPPP
                     FRNRRLVCQALSAFLTNVIYKQFPEQPESCQQMLDDITRHVSMQLDKIPQPSK"
     gene            complement(3392..3582)
                     /locus_tag="VAH76_002914"
                     /pseudo
     CDS             complement(3392..3582)
                     /locus_tag="VAH76_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
BASE COUNT         1304 a          735 c          649 g         1179 t
ORIGIN      
        1 ttgaaacagg tcagatacga cttcaataaa attgcttaaa ctgtatgtaa aaaaactttc
       61 atacaaatct gttgtgaaat cagtattatg ttcttcatta tattctttga cagcattttt
      121 ggctgcttgt aatgccgagt taatttcatc agagactcgt ttaaaataat tagcataatc
      181 ctcttgggtt ttaacttcca ttaatgaaat ggtcttaatc aaacatctgg caagctcatt
      241 tcgttgaatt aataattccg cttctttgcc tttatatgtt ttttcatctg cctgcaattg
      301 gtagctatcc aaaatttttt tataatagcg gtcggtaata tctttaaaaa tccacattat
      361 ctacctcttt aattagtgat ccagaaagta tttgcaaaaa ttgtcgctga ttatcaagaa
      421 caataacgtc ttccttttct attacgggcg atagtcaata ttgtaatgac agcaaacaca
      481 tgatttcctt gtctgatgta ttggtaatcc attgataact gccaagaatt ataaataaat
      541 gttcaaaaaa cagtaggaat agccatggtg tctaatagta actcattgta agttatagga
      601 aaagtatctg tattcggttt tttggaaata gttgaagaca aaaacactag agagaaacat
      661 acttacaagg cttaaaaaca gcattgattt acaaagcaat tatttggctc ttttctaaaa
      721 aagaacgata attaatcaag agtggtgagg ttaccaattt gcagaactca aataaggttt
      781 ttgctctgga cctcttacat agccaggata gaatcgctgc aacatgacct gatgaggcaa
      841 aataaagaaa agataattat tatatttttc caaaaagaca ttaatcatga aatcaaaata
      901 cattagctta tgcttttttt cactcatttt atggtttggc agtccaattc ttttggcaca
      961 accatccagg ctggataatt taattaatca actggttcaa ccatttattt atgcacatca
     1021 gactcccgga ttagccattg ccatttatta taatggaaag gattattatt ataattttgg
     1081 atttgctgac aggaaaagta agaagccagt cactaaaaat actatttttg aattggcatc
     1141 gattaccaaa atattcatta gcactcttgt cgctttggaa gttcagaaag gaaaattgaa
     1201 tgtaacagat tctgccgttc aatatacgcc cgaactttca aaaaccaatg gactaccaat
     1261 tgacagggtt caaattgtgc atctggcgac tcacacagca agctttccca aacaaatgga
     1321 gcaatttggt gttaataaag gcaatataca ggctttcttt atccgtctta aaacatggcg
     1381 tcctccagta aaaataggaa ctcattataa atattccaat attggttttg ggtatttggg
     1441 atacgtattg gaaaacgcct caaacgagac tttacctaat ctgttaaaat cgaatattac
     1501 caagccattg ggaatgtccc atactttttt caatgtacca aaaagtctgg ctaactatga
     1561 agctcaaggt tatcgaccta aaaataagcc agcgccttat tatacacctg ccaactttct
     1621 aggtggagga gctttacgct cttcttcagc tgacatgctg gtatttttaa aagcaaattt
     1681 aggaattcaa cctggaaccg catcaccaga attactttct gcgatgcagt atgcccaaca
     1741 accgtttttt gaagtcaacc caaattttgt tatgggatta ggttggcaaa gagttaaaag
     1801 agggaggcac attgtgatca ctaaaaatgg agcaaatcag ggatttagta cctttattgg
     1861 tttttctcct gcgaaaaaac tgggagtagt cgtattaatc aaccaagcaa gaggaaaggc
     1921 aactgcattg ggcaaccaaa tacttaatag ccttcttgat ttataattca tacattggca
     1981 caaaagttaa tactgccttt cataagcaag ccatacaaca ctttaaggct atttgtttgt
     2041 ctagaatgca ctcacagaca ttgacaatca aaatgttgtg caacatgatt acctttgtac
     2101 cttttctctg tatagcttgg tttacaacgt ggataggatg attttaatct gaagtaatgg
     2161 ctaacagaac aacaggtcaa accagcaact taaaataacc gaaaaaggga gtttcgatga
     2221 aaaaatccaa tcatgtacac tcattgttat tgcttaccgc tattactttt tcacctgtta
     2281 tcaaggcaag ccctgtaggt ctgattgtag atatctattg ccctactacc cagggaggac
     2341 caaatgtcat cactaatttt ggagattaca ttgctggtta tgggatggaa aatattttat
     2401 cacaaaacaa tcccatttat tttaaatcca tttcactagc ccacgatgtt ccagcccaac
     2461 ttggaaatta ttacaatgaa acaaccagct acaacagcac aacaggccaa gtcacttgca
     2521 gttacataag caataaccca acagaacctc gctttgctgt ggcgtatacc ttaaccaatg
     2581 gcaggggcgg ttctgtccaa tggcagtcag ggaattcaat caatatcatt ttcccagttg
     2641 gtctaaaaag ttaaccaaag tttactggat taaaatgcct gaatgtcttc ttcgtctccc
     2701 agcaactctt tctggaattg atttgctggg gagattgaca gaaattctgc aatcaaagcc
     2761 aaagccaaac caagcagaag aacatacctg ataattgggg tattcaagct gaaacgcatg
     2821 atttatttga tttcaactca ggccaaatta gttgatacag attactagcg ttttactttg
     2881 atggttgagg gattttatcc agttgcatac tgacatgtct ggtaatatca tcgagcattt
     2941 gttggcatga ctcaggttgc tctggaaact gtttataaat aacattagtc aaaaatgcag
     3001 acaaagcctg acaaaccaac ctgcgattac gaaacggtgg cggtaacttt tccacagatt
     3061 gagttaatgt tgacaataaa ttttgaccta taaaattcat tgccctggaa taatcttcag
     3121 cggttacagc ttcgcctggt tgagcgtttt cactattcac attcattttt tatcctgtca
     3181 gtgtgtgatc aagccggatt atagagtcta tctcgagcta cttcaacgac tgtttcagct
     3241 ctttccataa aaccagcacg attatccatt aaaacaacaa aatacctcat taacaggact
     3301 tacgaaaaac cccaaggtcg agacaaaaag tgtttttaat aaagagattg ggaggagcat
     3361 aagtcctgat taaaactcat ttatgatgta gttatggttt tacaatgaca acgggctctg
     3421 gtgtgccacc caaaagacta aagaccaaaa gcacaataaa cattacgata aagaggaaaa
     3481 acaaaatttt ggctatccct gttgcggctg atgcgacgcc ccaaatccaa ataaacccgc
     3541 gacaattgca acaataagaa aaattaaggc ccaatataac ataataaact ccctgttttc
     3601 agaataatcc tttcttcctg aattgttttt accgaagtaa aatcaagcta cccctactta
     3661 tgctcatctc acttataaaa aaacaaactg aaatacttat taatactcat catattaaaa
     3721 ttgtagacta tttatccaaa ataaagaaaa acaaacaaca acgcataaca acatgaagga
     3781 aataaactat ttaattgatt caggtttaaa atatttcata ttgtcctctt tcaaccatag
     3841 attgtccgct ttcgaccata aagccga
//
LOCUS       DATNSN010000218         3777 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.219, whole genome shotgun sequence.
ACCESSION   DATNSN010000218 DATNSN010000000
VERSION     DATNSN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.219"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1194
                     /locus_tag="VAH76_002915"
     CDS             <1..1194
                     /locus_tag="VAH76_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEO1387494.1"
                     /translation="INQDNNIKPDAPEKNVNQYNTTTYSNEQFAIQKLLLSHGQITLN
                     NKGRSTVLKNIQIGAEQFNLKNSPFSVQIKAKLTDAAFLQTAKANINFKGRVSLSPSI
                     IAELNSGISKSSIDGQLQIQNILLNQFAIKKINTTVKTHKRDIQFNPLTLSLYNGESI
                     GDMVYVIATQRLLLNQTATNLDGKQLIISLLKHPAISGNLDYSIHASIPLKTLSVENL
                     ISKGTITLKDGEIYNINLDQLLDNLKIKLNSLITETPENIKKLTQSADWDKNKNTQGN
                     TKFKLANFKFQLQTGTISSDSFLLQTDKLQVNGEGRINLLNQEINSSIKATLNDNSTD
                     NTLQKIQQALGGYFPLVVSGTVEHPIVLPDFKTISPVLSSLAIKSALTKPLKIIQKPL
                     KELIH"
     gene            1191..2258
                     /gene="mutY"
                     /locus_tag="VAH76_002916"
     CDS             1191..2258
                     /gene="mutY"
                     /locus_tag="VAH76_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO1387495.1"
                     /translation="MSTNSLNQSFSELLLDWYDQHGRKDLPWQLPRSPYRVWVSEIML
                     QQTQVQTVIPYFNRFIGHFPDIFLLANAEEDAVLSLWSGLGYYSRARNLHNTAKIICD
                     RYNGVFPEDLDTLIQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWLE
                     QAQVKKKLWELASSCMPNQRCADYTQAIMDLGATCCTNKNPQCPRCPIKNHCLAFQNK
                     KQHLYPTKKIKKQRPTLSQQFLVLHNEQNQVYLEKRPPTGLWGGLWCLPSIDNQACPI
                     QHIQLIYRLQGESPKLITRFKHSFSHFHLEIIALSIRIQPTNNFVSESSGQWFTKETL
                     PTLGLAKPTTLILSKLMVGDA"
     gene            2469..3200
                     /locus_tag="VAH76_002917"
     CDS             2469..3200
                     /locus_tag="VAH76_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387496.1"
                     /translation="MHLTREVNIMSILSAVSRVITNFSTEIKNRVVEVGSNLKKSTEE
                     FIGEVVIKAAVNRLKQGMLNALSPSDDTIKDGSNDLSTNNPSIEEVSSPTLNSKKKTE
                     ASTPLAGSTGIMQKLLGNIGLNSEQSIEVLSKHFNGAVERFTQDVSKAANEAVCEKIK
                     SIQPEDVIKGINGLIGNTLKVDPESGDNNISFRLESAVQILGLILATMGMLMLVSQTK
                     LPEQKTPEIEHLPTEEIQPPSLAIH"
BASE COUNT         1339 a          703 c          625 g         1110 t
ORIGIN      
        1 atcaatcaag ataataatat aaaacccgac gctcctgaaa aaaatgttaa tcagtacaac
       61 accacaacgt actccaatga gcaatttgcc attcaaaaat tattattaag ccatggacaa
      121 attaccctta acaataaagg acgttctact gtcttaaaaa atattcaaat cggagcagaa
      181 caatttaatt taaaaaactc gcccttttca gtacagatta aagccaagct aactgatgct
      241 gcttttttac agacggcgaa agctaatatt aattttaaag gtcgagtcag tctttctccc
      301 tcaatcattg ctgaacttaa ctcggggatt agcaagtctt ccatagacgg gcaattacaa
      361 atacaaaata ttttattgaa tcaattcgcc atcaaaaaaa tcaataccac tgttaaaact
      421 cataaaagag acattcaatt taatcctctt actttgtcac tctataatgg agagtcgata
      481 ggagatatgg tctatgtaat cgctacccaa cgattgttac ttaatcaaac ggcaaccaat
      541 ttagatggaa aacaattaat catttcttta ttaaaacatc cagcaattag cggtaattta
      601 gattactcaa ttcatgcttc aatacccttg aaaacactca gtgtcgagaa ccttattagt
      661 aaaggaacca ttacacttaa agatggggag atttataata tcaatcttga tcaattactt
      721 gataatctaa aaataaaact taattcgctt ataaccgaaa ctccagagaa cataaaaaaa
      781 ctgactcaat cagcggattg ggataaaaat aaaaacacac aaggcaatac caaatttaaa
      841 ttagcaaatt ttaaatttca gttacaaact ggaacaatta gcagcgattc atttttgctg
      901 cagaccgaca aactccaggt gaatggagaa gggcgaatta atctgctaaa tcaggaaata
      961 aattccagta tcaaggccac actaaacgat aatagtactg acaatacttt gcaaaaaatt
     1021 caacaagccc tgggcggata tttcccattg gtagtatctg gcacagttga acatcccata
     1081 gttttgccag attttaaaac gattagtcct gtattaagtt ctttagcaat caaatcggct
     1141 ttgacaaaac ccttaaaaat aatacaaaaa ccgcttaaag agttaatcca ttgagtacaa
     1201 actctttaaa ccaatctttc agtgaattac tgcttgattg gtatgaccaa catggacgta
     1261 aagatttacc atggcaatta ccccgttctc cctacagagt ttgggtgtcg gaaatcatgt
     1321 tacaacaaac tcaagtccaa acggttattc cttattttaa tcgttttata gggcattttc
     1381 ccgatatttt tttacttgcc aatgcagaag aagacgcagt gctctctctc tggtcagggt
     1441 taggctatta cagtcgtgcc agaaatcttc ataacacagc caaaataatt tgtgaccgat
     1501 acaatggagt atttcctgaa gatctggata ctttaatcca actgccagga ataggaccat
     1561 cgaccgcagc agctatacta tcacaagctt ttaataaacc agctgctatt ttagatggaa
     1621 atgttaaacg agttttatct cgattttttt taattgaagg ttggctggaa caagctcagg
     1681 ttaaaaaaaa attatgggag cttgcatctt cctgtatgcc caatcaacga tgtgcggatt
     1741 atacccaggc aattatggac ttaggggcaa catgttgtac aaataaaaat ccacagtgcc
     1801 cgcgttgccc cattaaaaat cattgtctgg cctttcaaaa taaaaagcaa catctttatc
     1861 caacaaaaaa aataaagaaa caacgtccaa cattatcaca acagttcctg gtgttgcata
     1921 atgaacaaaa tcaggtttat ttggaaaaaa gacctcccac agggctatgg ggagggttat
     1981 ggtgtttacc cagtatagat aatcaagctt gccctattca gcatatccaa ctcatctata
     2041 ggttgcaggg agaaagccca aagctaatta cccggtttaa acacagcttc agccattttc
     2101 atttggaaat aattgcatta agtataagaa tacagcctac gaataatttt gtctctgaat
     2161 ccagtggaca atggtttaca aaagaaacat tgccaacact gggtcttgcc aaaccaacca
     2221 ccttgatact atccaaatta atggtgggcg atgcctaatg aagagctgtt taaaaaacag
     2281 caagtgctgt attaactctt aaatccggat aagccaatac aatgtaacaa aaaatttaca
     2341 aagagtttat ttattcatta tttaataaat aatgaacaat tacctttaat ttacattttt
     2401 ttgtaaagaa atgttacaaa cttaacattt tcttaatgtt cacttaatgt tctctcctta
     2461 ttatttgttt gcacttaaca agagaggtaa acattatgtc tatattatct gctgtttcgc
     2521 gtgtcattac taatttttcg actgaaatta aaaatagagt tgttgaagta gggagtaatt
     2581 tgaagaaatc aacagaagag tttattggcg aggtagtcat aaaagctgct gttaaccgtt
     2641 tgaaacaagg gatgttgaat gctttgtctc cttccgatga caccatcaaa gatggtagta
     2701 atgatttatc aacaaataac cctagcattg aagaggtatc cagccctact ttaaactcaa
     2761 agaaaaaaac agaggcaagt accccgttgg ctggcagtac aggtataatg cagaaattgt
     2821 tgggtaatat tggcctcaat tctgaacaaa gtatagaagt gttgtccaaa cattttaatg
     2881 gtgctgttga gcgctttact caggatgttt caaaggctgc aaatgaagca gtgtgcgaaa
     2941 agatcaaaag tatacagccg gaagatgtga taaagggcat taatggttta atcggcaaca
     3001 ctctcaaagt agacccagag tccggggaca acaacatatc attcagactt gagagtgctg
     3061 tacaaatcct gggactcatt cttgcaacca tgggtatgct gatgcttgta tctcaaacca
     3121 aattacccga acagaaaact ccggagatag aacatttgcc aactgaagaa attcaaccgc
     3181 cttccttagc tattcattaa gacctgttga caacttgttc cccaagctgc gtagcgcaaa
     3241 tagtttacgg cacgtagctt ggggaactgg cattacattc tatacattcc tcaagtattt
     3301 gctttgatta ttgaatttgt ccttttcatg aaaatctagc actttcttta gcatagtgat
     3361 tacgctagct actgacgtat tttttcaaac attagcctta cctgtagatg attattcttc
     3421 tttaaaagtc taataccgta acagagcaag tataaaaata tgtgagctac agagcataaa
     3481 aaataaaaat ttttatgcca ttatggtatc aatacaaaca aaccagactg atgaaaaact
     3541 ataaggtcat ggcaaaaaat gcttttaata agtgagcata aataaactaa atgactgaat
     3601 taaacatatt ttttaatgaa gatattgagg gatttcgtaa gtcctggcag aacctgaata
     3661 taaagtgaaa cttaatctct ataaattata tcctataaac cattctcgta ttattatcat
     3721 taaaaaatat cgagtacaaa aatgaatccc attctgaatt tgattatcag ttttatt
//
LOCUS       DATNSN010000219         3717 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.220, whole genome shotgun sequence.
ACCESSION   DATNSN010000219 DATNSN010000000
VERSION     DATNSN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.220"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..773
                     /locus_tag="VAH76_002918"
     CDS             <1..773
                     /locus_tag="VAH76_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEO1387497.1"
                     /translation="YGFTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFEN
                     EEETQVAIASILTYVGFSIAVISGLLVAGFNFTGLAIIAGALSVGIGLGLQSIVNNFV
                     SGIILLIEKPIRPGDRINIDGVEGFVKKIRVRSTQIITPVNEDIIIPNSDLITRRVVN
                     YMLTDNYWRVNCEVSVAFGTNLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSA
                     LTFQLWCMIKDVNKKSTVKSELNFSIEEAFRKHDISIT"
     gene            946..3090
                     /locus_tag="VAH76_002919"
     CDS             946..3090
                     /locus_tag="VAH76_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO1387498.1"
                     /translation="MIKKIHRWLPLLVLIGLLVLFFSLHLDKYLSFNALRENHALLIA
                     WTQTHYFIAPLIFIVFYTTAVAISIPGAVLLTLTGGFLFGVFWGVLFVVISATLGATI
                     LFFAVRTALGDWFAQKASGWIERMRQGFQHNAFSYLVTLRLIPLFPFWAVNIVPAFLN
                     IRAKTFITATFIGIIPGTTVYVMVGNGLSQIFAANQTPNLGIIFELQILGPLLALAVL
                     SLMPVLYQFLTRKHQNHHSFSDISRVINCDLAIIGGGAGGLSLASGCSQLGLKVVLVE
                     SGKMGGDCLNYGCIPSKSLLAAAKTFYYAKHATHFGVHTEAIKVNFQQVMQHVHQIID
                     NISEHDSVQRFESLGVQVIKQVGKFLTPDTLQAGDSIIKAKRFVIATGSSPFIPPIPG
                     LDTVSYFTNETIFDLKEQPEHLIVIGGGPIGCELAQAFAMLGSKVTLLEGLNLLPKDD
                     PDCVAVLRTQMKSMNILIYEQIEITQINSHPDAGISVCFEFQNTQFTITASHLLIATG
                     RRANVTPLDLEKAGVKFTSKGVEVNKYLQTSNKKIYALGDVTGLYQFTHMASYHAGIV
                     LRNIVFKLPSKVDYRAIPWVTYTDPELAHVGLGASDALKHPDSQIIEWPFVDNDRAQT
                     EHTLNGKIKIITDNKARILGVTIVGPHAGELILPWVMAIREKKNLRSFTDVIVPYPTL
                     SEISKRVAGNFYAPKLFSNKTRTLVRWLQKIG"
     gene            3181..>3717
                     /locus_tag="VAH76_002920"
     CDS             3181..>3717
                     /locus_tag="VAH76_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEO1387499.1"
                     /translation="MKQDFSAKIITLREVYDISYKLAKIITQSAYQFDVVVAIARGGF
                     PAARFICDFLNIRQLGSMQISHYSGGAQKYADTQVVSPANINISGKSVLVVDDVNDTG
                     ETLKSASDYIQSLNPALYKIAVLYEKSSTVFKADFVSKHQKKWKWLMYQWAVTKDILT
                     FLKQDNMLESNEDEVCKHL"
BASE COUNT         1119 a          668 c          715 g         1215 t
ORIGIN      
        1 tgtatggctt tactctggca aatattacaa tttatccaac gcgcattctc ttcgggatca
       61 taattttttg ttttttatat ttggtgtctc gtgccatttc aacttcaatc agtcgtcacc
      121 aacagttcga aaacgaagaa gaaactcagg tagctattgc ttccattttg acttatgttg
      181 gattcagtat tgctgttatt tcaggcttac tcgtcgcggg ctttaatttt actggtcttg
      241 ctattattgc tggagcgctt tctgtaggaa ttggtcttgg actgcaaagc atagttaata
      301 attttgtatc aggtataatt ttgcttattg agaaaccaat aagacctgga gatcgaatta
      361 atatcgatgg tgttgaagga tttgtaaaga aaatcagagt cagatcaaca caaattatca
      421 ctccagtaaa tgaagatatt attattccaa actctgattt aattacacgc cgcgttgtca
      481 attatatgct gacagataat tattggcgtg ttaattgtga ggttagcgtg gctttcggga
      541 caaacctgaa tcttgttaaa gacatccttt tggagatcgc taataagcat gatgatgttg
      601 taaaaagtgg tagaaacaaa cctgttgtgt tatttaccag ttttgccgac agtgcattaa
      661 cttttcaact ctggtgcatg attaaagatg taaataaaaa atcgacagtc aaaagtgaat
      721 taaatttctc tattgaggag gcttttcgca agcatgacat ttcaataaca tagtcttaat
      781 gtgattttca tatttgaatt ggagattaaa tacttcctct actctgaaaa ataatgctta
      841 gtatcattaa accctatcga taattaaggt atgacctcaa agtaaattcc tgttaggatc
      901 ttcatataat ttatatcagt ttatcgggtt aaaggaataa catgaatgat taaaaaaata
      961 catcgttggt tgcctttgct tgtattaatc gggctattgg tgcttttctt cagcctacac
     1021 cttgataaat acttaagttt taatgcatta agagaaaatc atgctttact cattgcgtgg
     1081 acccaaactc attattttat tgcgccactg atttttattg ttttttatac cactgctgta
     1141 gccatctcta ttccaggagc agttcttctt actttaactg gaggattttt attcggagta
     1201 ttctggggcg tgttatttgt agtcatcagt gctacgcttg gggccaccat actgtttttt
     1261 gcggttcgca ctgctttggg cgactggttt gcccaaaagg cgtctggatg gatagagcgt
     1321 atgcgtcagg gatttcagca taatgctttt tcttatttag ttacactgcg attaattcca
     1381 ttatttccct tttgggcagt taatattgta ccggcttttt taaatattcg agcaaaaaca
     1441 ttcattacag cgacttttat tggcatcatt ccgggaacaa cagtatatgt aatggttgga
     1501 aatggtctaa gccaaatatt tgcagccaat cagacgccta atcttgggat catatttgaa
     1561 ctgcaaatat taggtccact gctcgcgttg gctgttctgt ctctaatgcc agttctttat
     1621 caattcttaa ctcgcaagca tcagaatcat catagtttct cagatattag tcgagtaata
     1681 aattgtgatt tagccattat aggcggtgga gctggaggtt tgagtttagc ttccggttgt
     1741 tcacaattag gactcaaagt tgttttggtt gagtcaggga aaatgggagg agactgttta
     1801 aattacggtt gcatcccatc caaatcctta cttgctgcag ccaagacgtt ttattatgca
     1861 aaacatgcaa cacattttgg agtacatact gaagcaataa aagttaattt tcaacaagta
     1921 atgcaacatg tgcatcaaat catagacaac atttctgaac acgattctgt ccaacgtttt
     1981 gagtcgctag gagtacaggt tattaaacaa gttgggaaat ttttaactcc cgatacatta
     2041 caagctggcg atagtattat taaagcgaag cgtttcgtga ttgcgacagg ctcatcacca
     2101 tttatcccac ccattcccgg attggataca gtctcttact tcaccaatga aacgattttt
     2161 gatttaaagg aacaacctga gcacttgatt gtcataggtg gtggccctat tggttgtgaa
     2221 cttgctcaag cgttcgccat gctcggttct aaagttactc tgcttgaagg attgaatctt
     2281 cttcccaagg acgatcctga ttgtgttgct gtcctacgca cacagatgaa atcgatgaat
     2341 atcctcattt atgaacaaat cgagattacc caaataaact ctcaccctga tgcgggtatt
     2401 tccgtatgtt ttgaatttca aaacacacaa tttacaatta cagccagtca tctgctcatt
     2461 gcaactggac gtcgggcgaa tgtaactcct cttgatttgg aaaaagcagg ggttaaattc
     2521 accagtaaag gtgtcgaagt aaataaatac ttacaaacca gtaataaaaa aatctatgct
     2581 ctgggtgatg taacagggct ttatcagttt actcatatgg cgagttatca tgccggtata
     2641 gtgcttcgta atattgtttt taaactaccg tctaaagtgg attatagagc gattccatgg
     2701 gtcacttata ctgatcccga attagctcat gttggactag gagccagtga tgccttgaag
     2761 catcctgatt cccaaataat cgagtggcca tttgtcgata atgatcgagc acaaactgag
     2821 catacattaa atggaaaaat aaaaatcatc acggacaaca aagcaaggat ccttggagtc
     2881 accatagttg gcccacatgc cggagaactc attttacctt gggttatggc gattcgtgag
     2941 aaaaaaaacc tccgaagttt tactgatgta atcgttcctt accctactct gagtgaaatc
     3001 agcaaacgcg ttgccggtaa tttttatgca cctaaattat tttccaataa aacacgaaca
     3061 ttagttcgct ggttgcaaaa aattggataa gtagattttt aatcaactat atgtttttta
     3121 tgtattatat atactaataa aactctataa tgatattaat ctatcaacca gattattacg
     3181 atgaaacaag atttttccgc aaaaataata acactgcgag aagtttatga catcagttat
     3241 aaactggcaa aaattattac ccaatcagct tatcaatttg atgtagttgt cgctattgca
     3301 agaggaggtt ttccagcagc acgttttatc tgtgattttt tgaatatcag acagttaggc
     3361 tcaatgcaga ttagtcatta ttctggtgga gcccaaaaat atgccgacac tcaagtggtg
     3421 tctcctgcca atattaatat ttctggaaaa agcgtattgg ttgttgatga tgtgaatgat
     3481 acaggtgaaa cactcaaatc ggcttctgac tatattcaat cactaaatcc tgctttgtac
     3541 aaaatagccg tactttatga aaaatcatcg acggtattca aagctgattt tgtaagtaag
     3601 caccaaaaaa aatggaaatg gctaatgtat cagtgggcgg taacaaagga tatcctcact
     3661 tttcttaaac aggataatat gctagaaagc aatgaggatg aggtttgcaa gcattta
//
LOCUS       DATNSN010000220         3663 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.221, whole genome shotgun sequence.
ACCESSION   DATNSN010000220 DATNSN010000000
VERSION     DATNSN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.221"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            108..488
                     /locus_tag="VAH76_002921"
     CDS             108..488
                     /locus_tag="VAH76_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387500.1"
                     /translation="MFRYFKQGWHGDLKFSEVLFGSGGDYFLLEGGLAYIGFYILFAI
                     LLITSKPLSLDNILAFALFSYGIVLYIWLIKAFWGSANHCSNKISAVLIRTFTILLPL
                     ISIVLFFLIIIYYLVTAIIDALSG"
     gene            541..1668
                     /gene="cfa"
                     /locus_tag="VAH76_002922"
     CDS             541..1668
                     /gene="cfa"
                     /locus_tag="VAH76_002922"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEO1387501.1"
                     /translation="MDIKKNKAKTFVNYMLQLANININGQTPWDIQIYNDEFYSRLLR
                     DADLGLGESYMDGWWDCQRIDLFISKLINANLESKIKINFKLAFNVFLSKILNLQTQK
                     RALHVGRQHYDRGNDLFQLMLDSNMNYTCGYWRKAENLEQAQLDKLDLACRKLCLKPG
                     MKLLDIGCGWGGLAKYAAENYGVSVVGITISQQQYELAKTRCAHLPVEIRFQDYRDLN
                     EKFDRIVSLGMFEHVGYKNYRKYMEIVHQCLNDDGLFLLHTIGSNESVTKATPWISKY
                     IFPNGMIPSIMQIGQASEKLFVMEDWHNFGADYYKTLMAWHENFNQGWEQIKSQYSEK
                     FFRMWNYYLLSCAGAFDARMLQLWQIVFSKKGIQGGYSAPR"
     gene            1833..2159
                     /locus_tag="VAH76_002923"
     CDS             1833..2159
                     /locus_tag="VAH76_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387502.1"
                     /translation="MPKNNEPSGEELKKAGIVVGITNLLLWPFYYADSRWGLFASIFA
                     TSAVLYASHEVGESKRPVENSLARLGLFQQRGASNAEIENAFNNTVTGAAAIYDWLVP
                     GSNGPK"
     gene            2324..>3663
                     /locus_tag="VAH76_002924"
     CDS             2324..>3663
                     /locus_tag="VAH76_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEO1387503.1"
                     /translation="MKFRTKLFLGLLLIVIITNGTFLLLSYQYGKETVYKEVGSSALS
                     IAAATAVLMNPEDIQKFNPDTSENSPLYKDLEKKLLEVRNANRRKDVYVNFIYSLFPD
                     SNNPKVLRFAVDPEENIIDKTELGTVMKLKTESGQAININYESPIVLPEFVEDIWGTW
                     LTALYPIKDAQGKYLGSIRVDVDADSVNQRFHFLLYSGIAVFISTFLFAMFLGWLLFQ
                     WFNKPLLKITTALKNIAQGDLEQHLDIKTKDEFAEVGRVINEMTEGLRQRNMLQVSLT
                     RFISHALAEKIVKSGELPQVFSERRKVTIMVCDIRDFTTISERIKPELVVDFLNHFFE
                     KMIEAITSQHGILDKYLGDGFLAIFGSPDDDAYQEDHAIHAALKMREAMKSLNVDWAK
                     ILGAEIQIGIGINTGSAIVGNIGTDIHMEYTAIGDTVNLASRIENATKKLNTDIL"
BASE COUNT         1139 a          639 c          722 g         1163 t
ORIGIN      
        1 ttttttaaca aagagattgg gggttttagt aaatcctgaa aaattctttc atccattgtc
       61 ctggcttggc ttataataaa aattataaat aacataacag gatccgtatg tttcgttact
      121 ttaaacaggg atggcatgga gatctgaaat tttctgaagt tctgtttggt tcaggtggtg
      181 attatttttt gcttgaaggc ggcctggcgt atattggatt ctatatttta tttgccattt
      241 tgttaataac atctaaacca ttatccctgg ataatatatt agccttcgct ctcttctcct
      301 atggcatagt cttatacatc tggttaatta aagcattttg gggatcggcg aatcattgct
      361 caaataaaat ttcagccgtt ttgattcgta cctttaccat tcttttgccc ttgatttcga
      421 ttgtgttgtt ttttctgatt attatttatt atctagttac agccataata gacgctttga
      481 gtggctgatg agttgcattg attgaatgtt tcaacatcct ttataaaaaa ggagtttgat
      541 atggatatta aaaaaaacaa agcaaaaaca tttgtcaatt acatgcttca gctagccaat
      601 atcaatatca atggccagac accttgggat atacaaattt ataatgatga gttctattcc
      661 agattattgc gtgatgccga tttgggtctg ggtgaaagtt acatggacgg ttggtgggat
      721 tgtcaacgaa ttgatttatt tatttcaaaa ctgattaacg caaatcttga gtctaaaatt
      781 aaaattaatt ttaaattggc atttaatgtc tttttatcaa aaatattgaa cctgcaaacc
      841 caaaaacgtg ctctacatgt tggtagacaa cattatgata gaggaaatga tctgtttcag
      901 cttatgcttg atagcaacat gaactacact tgcggttatt ggagaaaggc tgaaaatttg
      961 gagcaagccc aactcgacaa gctcgatctg gcctgcagaa aactctgcct taaacccggt
     1021 atgaaattgc tggatatcgg atgtggatgg ggaggacttg caaaatatgc tgctgaaaac
     1081 tatggggtat cagtagttgg cataacgata tcacagcaac aatacgagtt agctaaaacg
     1141 cgctgtgctc atttgcctgt tgaaatccga tttcaggatt atagagactt aaatgaaaaa
     1201 ttcgatcgta tagtctccct agggatgttt gagcatgttg gttacaagaa ttaccgaaaa
     1261 tacatggaga ttgtccatca atgtttaaat gatgatggtt tgttcttatt acacactatc
     1321 ggttccaatg aaagtgtcac aaaagccaca ccctggattt caaaatatat ttttcccaat
     1381 ggaatgattc catccatcat gcaaataggc caagcttccg aaaaattatt cgttatggag
     1441 gattggcata attttggagc tgattattac aaaactttaa tggcatggca tgagaatttc
     1501 aatcagggat gggagcaaat taaatcccaa tatagcgaaa aattctttcg tatgtggaat
     1561 tattatttgc tctcttgtgc tggcgctttt gatgccagaa tgttgcaatt atggcagatt
     1621 gttttttcaa agaaaggaat tcaaggagga tactctgcgc cgaggtaaat ggtttaaacg
     1681 atttattccg catcgtaata ccttcaccag caaacctggt tttccatgcc atgaaattgg
     1741 caagattatt catatatccc gttaattttg cttgcaattt ttattatctg gaatatagtt
     1801 tgaactgaaa ttttgtctaa aaaggaatta atatgccaaa aaataatgaa ccaagtggag
     1861 aagagctcaa aaaagcaggg atagtggtag gaataacaaa cctgctttta tggccatttt
     1921 attatgccga ctcgagatgg gggttgtttg catccatatt tgctacttcc gcagtacttt
     1981 acgcttctca cgaagtcgga gaaagtaaac ggcccgttga aaattcattg gcgcgacttg
     2041 gtctcttcca gcagcgtgga gccagcaatg ctgaaattga gaatgctttt aataatacag
     2101 ttacaggtgc agctgcaata tatgattggc ttgtccctgg ctccaatggc cccaaataat
     2161 aagtatcctg ccttatatta aattatattt ttggggggaa gtattatttt atccaatacc
     2221 acgccattct ggagattggt caagatctcc agaaatctgt tgtctttact tctcactgcc
     2281 tttatccaat acttataatt aatctgctaa tggtggataa ataatgaaat ttcgtactaa
     2341 gttatttctt ggtctacttc tcatcgtaat cattactaac ggcacatttc tgttgttatc
     2401 ttaccaatat ggtaaagaaa cagtatacaa agaagtaggc tcatcagccc tatccatagc
     2461 tgcggcaaca gccgttctga tgaatccgga ggatattcag aaatttaatc cggatacgtc
     2521 tgaaaactca ccactttaca aagatcttga aaaaaaactt cttgaagttc gtaacgcaaa
     2581 tcgtcgcaaa gatgtgtatg tgaatttcat atatagttta tttcccgact ctaataatcc
     2641 caaggtactt cgctttgccg tagatcccga agaaaacatt atagataaaa ctgaattggg
     2701 aactgtcatg aaacttaaga ctgaatcagg gcaggcaatt aatataaatt atgaatcccc
     2761 tatcgttctg cctgaatttg ttgaagacat ctggggtaca tggctgaccg ctttataccc
     2821 aataaaagac gcacaaggga aataccttgg aagtatccga gtggacgtag acgccgattc
     2881 ggttaaccaa agatttcatt tcttgctgta ctcaggtatt gccgtcttta tctcaacatt
     2941 tttatttgcc atgtttttag gttggttact ttttcagtgg ttcaataaac ccttattaaa
     3001 aattacaacc gcactcaaaa atattgccca aggtgatctt gaacaacatc tcgacattaa
     3061 gacaaaagat gaattcgcgg aagtgggtcg agtaatcaat gaaatgactg aaggattgag
     3121 acagcgtaac atgctgcaag tgagcttaac acgttttatt tctcatgctt tagcagaaaa
     3181 aattgtgaaa tccggtgagt tgccgcaagt atttagcgag aggcgtaaag tcactattat
     3241 ggtgtgtgat atacgcgatt tcacaaccat ttcggagcgt ataaaacctg aattggtagt
     3301 tgatttttta aatcattttt ttgagaaaat gatagaagcc attacctcgc aacatggaat
     3361 attggataaa tatcttggag atggtttctt ggcgattttc ggttcacctg atgatgacgc
     3421 ctatcaagaa gaccacgcta tccatgcagc tttaaaaatg cgagaagcga tgaaatcact
     3481 caatgtcgat tgggcaaaaa tattaggcgc tgaaatacaa atcggtattg gtatcaatac
     3541 aggcagtgcc attgtcggga atattggaac cgatatacat atggaataca cagctattgg
     3601 tgatactgtc aatttggcgt cacgaataga aaatgccacc aaaaaactca atacagatat
     3661 tct
//
LOCUS       DATNSN010000221         3631 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.222, whole genome shotgun sequence.
ACCESSION   DATNSN010000221 DATNSN010000000
VERSION     DATNSN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.222"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..596
                     /locus_tag="VAH76_002925"
     CDS             <1..596
                     /locus_tag="VAH76_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387504.1"
                     /translation="GSPDSAVCIEVLLNTLSKEGKSIDDRQRAAYISRAFEGNKPHMI
                     ERFIKGISDEKRLEFLISAIKKDQPHMVELFIKDLPKELLNTISLSLPAIEKMNVATL
                     KILEKNGMKFSEMEKAIIAKKSHQPVGFLLSIGVALVKFTDFCKEVLFKNESVTYDKN
                     KFQAFKSQLNSIKTQNSLANNEPVEAQQNDHVAVYRP"
     gene            complement(953..2281)
                     /locus_tag="VAH76_002926"
     CDS             complement(953..2281)
                     /locus_tag="VAH76_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEO1387505.1"
                     /translation="MNCFRNLRLLFYLIYVGDLCANPLKLTSPLSHSLSREYQDTSSI
                     SSNPAAVNVRTGSGILQQYIEKKLGIQDSHGISTQFAWIGDTNELFGGGIPNAKDNTS
                     NSVGLLDLTIQMEHFNGWKGGLFSAQFLQQNAQNTNGPAGVIQGYNSLPDVPPYNRSE
                     LYALWYRQALFDDTFFIRLGKTITTLDFNNVIKPVQLSKDEPNIPAVTSLIYTPIFIN
                     PSVDGLMPGYTNTAYGITATYTPIKSWYASYGIYDGNLARGKQTGLSGPTFNGNYFQV
                     GETGSVWLLGKDKKPGTIGIGLWHQTGLIKQGNLIQRDSSGTYLFGSQRLWYRHPGQD
                     ISGISGFYQYGINNSRVLPMKQSIGAGLTAFGLIANRKADSIGSGFSLAWLNQRVTNR
                     KTELMFQIYYQAKLFNFIYLEPVLSYIPTPGQSSHLNPASAGTLRAIVLL"
     gene            2435..>3631
                     /locus_tag="VAH76_002927"
     CDS             2435..>3631
                     /locus_tag="VAH76_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEO1387506.1"
                     /translation="MLNKIRAFSLLIAIVFFSNVSAETYKVPATKDTVTLGLFTLDKT
                     PVVKVHSGDSVSLETWNSCLHEMVFNKTTPAKIAKFYTKHDIQKRRGMHSLTGPVYVE
                     GAEPGDVLEIRVLDIKLNDFGYNFLSPTQGILSEFTKPRIMYFKYDKEKNTTEFTKGI
                     CLHLKPFPGIIAVAPPLDWPDGWPVNLETQMGQPVPGQFNSVPPGPFGGNLDEPSLQV
                     GSILYLPVFQKGALVWTGDSHAMQSNGEIDVTALETSFEGITLQFIVRKDLKAEGVFD
                     KSKRNGNSLFTWPIVENSEEWIIIGLHENLFEAMQLASKNAIEFLVRTQGLSSQESYQ
                     FLSMVGNFDIPEAVNVIKSVAVHIPKEIFKNTGKMTSLSSEGKFPLTTPRINENTTCV
                     PQEGKII"
BASE COUNT         1179 a          658 c          696 g         1098 t
ORIGIN      
        1 atggttctcc tgatagtgcg gtttgtatag aggtgctgct taatacatta tcgaaggagg
       61 gtaaatcaat agatgataga caacgcgcag cttacatatc gcgtgcattc gaaggaaata
      121 aaccacatat gatagagcgc ttcattaagg gaataagtga tgaaaaacgg ttagaatttt
      181 taataagcgc aattaaaaaa gatcagccgc atatggttga actcttcatt aaggatttac
      241 caaaagaact tttaaatacc atatcgttga gtttacctgc aatagagaaa atgaatgtgg
      301 cgacgttaaa aatactggaa aagaatggta tgaaattttc tgagatggaa aaagccataa
      361 ttgctaaaaa gagccatcaa ccagttggtt ttttactgtc aattggtgtt gccttggtaa
      421 aatttacaga tttctgtaaa gaggttttat ttaaaaacga aagtgtgacg tatgataaaa
      481 ataaatttca ggcgtttaag tctcaattaa atagtataaa gacccaaaat agtttagcga
      541 ataatgagcc tgttgaggca cagcaaaatg atcatgttgc tgtgtataga ccttgattgg
      601 cgcgctcgga gagactagaa ccccaagtac tttagtgcga agctgatgat gttataattt
      661 cttataaata ttgtgaaccg tcaaattcac ctgaaatagc ctattaccgt gaggtgccgc
      721 tattatgaag tctgttatca actggctaaa aaaatatttc caatattgaa cattcaaggc
      781 atcgctctat attcaatttt atggtcaata tattggccgg tttagctgct tatgcactta
      841 aatcaaagaa gctctcttta aatattaaac gatcttttat ggctgttgtt tgagcagtta
      901 tatcgaattc atggtagata ggttaatcaa agagaacaca caatttgacg gtttaaagta
      961 acactatggc tcgtaaggtc ccagctgatg ctggattgag atgactactt tgccctggag
     1021 tagggatata agagagtact ggctcaagat aaataaaatt aaataatttt gcctggtaat
     1081 aaatctgaaa cattagctct gtttttctat tggtaactcg ctgatttagc caagccaaag
     1141 aaaacccgga tcctattgag tctgcttttc tattagcaat taatccgaat gcagttaaac
     1201 ctgcgccaat agattgtttc ataggtaata ctctagagtt attgatccca tattgataaa
     1261 aaccagaaat cccgcttata tcttgaccag gatgcctata ccataatcgt tgagaaccaa
     1321 ataaataagt cccgctagaa tctctttgga tcaagttccc ctgcttaata agaccagtct
     1381 ggtgccacaa tccaattcca atagtgcctg gttttttatc tttacctaaa agccaaacac
     1441 ttcctgtttc acccacttga aagtaatttc cattaaaggt tggccccgat aaccctgttt
     1501 gctttcccct tgctaaatta ccatcgtaaa taccataaga cgcataccat gatttaattg
     1561 gcgtataggt tgctgtaata ccataagccg tattggtata ccccggcata aggccatcta
     1621 cagaagggtt tataaatatg ggtgtataaa ttaaacttgt tacagcagga atgttaggct
     1681 catctttgct taactggacg ggcttaatca cgttgttaaa atctaaagta gttatagttt
     1741 taccaagcct aatgaagaaa gtatcatcaa acaaagcttg tctataccaa agagcataga
     1801 gctctgaacg gttatagggt ggaacatcgg gtagcgagtt gtacccttgt ataacacctg
     1861 cagggccatt tgtattttgc gcattttgtt gtagaaattg tgcgctgaaa agcccacctt
     1921 tccagccatt gaagtgctcc atctgaatgg ttaaatccaa aagccctacg ctatttgagg
     1981 tgttatcttt tgcattgggt atccctccgc cgaacagctc attagtatcc cctatccaag
     2041 caaattgagt tgaaatacca tggctatcct gaatccctaa tttcttttca atgtattgtt
     2101 gtaatattcc tgagccagtt cggacattaa ctgcggcggg gtttgaacta atgcttgaag
     2161 tatcttggta ttctctactc aatgaatggc ttaatggact tgttagtttt aaaggatttg
     2221 cacaaaggtc tcctacatat attaaataga acaacaatct taaattacga aaacaattca
     2281 taatagttct tttgatgaat ctttaagata ctgtatcaca ataaatttta tactcaacgg
     2341 acttgaattt ttatgaaatt catactaatc ataataggtt atttgatgca aactatggca
     2401 aacttccttc attagccatt cttaaggact agaaatgcta aataaaatcc gagctttctc
     2461 tttattaata gcaatagtat ttttctcaaa tgtttcggca gaaacataca aggtcccagc
     2521 tacaaaagac acggtaacac taggtctttt tacattggat aaaacacctg tagttaaagt
     2581 tcactctgga gattctgtgt ccttggaaac ctggaatagt tgtctgcatg aaatggtctt
     2641 taataaaaca acgccagcta agattgcaaa attctataca aaacacgaca ttcaaaaaag
     2701 acgtggtatg catagcttaa ctggacctgt ttatgttgaa ggagccgagc ctggggatgt
     2761 acttgagatt cgggtattag atatcaagtt aaatgatttt gggtataatt ttttaagccc
     2821 tacacaaggg attttatcag agtttaccaa accaagaatc atgtatttca aatatgataa
     2881 agaaaaaaac accacagaat tcaccaaggg gatatgctta catctaaagc cttttccggg
     2941 aattatcgca gtagctccac ctctagattg gccggatgga tggcccgtga atttagaaac
     3001 tcagatgggt caacctgttc caggacaatt caattcagtc cctccaggtc cttttggagg
     3061 gaacctggat gaaccaagct tgcaagtagg ctccatcctt tatttacccg tttttcaaaa
     3121 aggtgcatta gtttggacag gagattcaca tgccatgcaa agtaacggcg aaattgatgt
     3181 cacagcacta gagacctcct ttgaaggaat caccttgcaa tttattgtaa gaaaagatct
     3241 aaaagctgaa ggggtatttg acaagagcaa gcgaaacgga aacagtttat tcacatggcc
     3301 cattgtcgaa aattcagagg agtggattat aataggttta catgaaaatt tgtttgaagc
     3361 catgcagctt gcctctaaaa atgcaattga atttttagta cgtacccagg ggctatcttc
     3421 acaagaaagt taccaatttt tgagtatggt aggtaatttt gatattcctg aagcagtcaa
     3481 tgttattaaa agcgtagccg ttcatattcc taaagaaatc ttcaaaaaca ctgggaagat
     3541 gacttcgctt agctctgaag gtaagttccc tctaacaacg ccaaggatta atgaaaatac
     3601 aacctgcgtg cctcaagaag gcaaaattat t
//
LOCUS       DATNSN010000222         3618 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.223, whole genome shotgun sequence.
ACCESSION   DATNSN010000222 DATNSN010000000
VERSION     DATNSN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.223"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            40..285
                     /locus_tag="VAH76_002928"
     CDS             40..285
                     /locus_tag="VAH76_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387507.1"
                     /translation="MNVHTMCFTRVLRYAVVTSILFMAVSFTSMANAAQGCGEGYHRA
                     IHNGTCVLNYPGPFATPAPAHPGCWRNMWGQLRCYRY"
     gene            complement(387..1424)
                     /locus_tag="VAH76_002929"
     CDS             complement(387..1424)
                     /locus_tag="VAH76_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEO1387508.1"
                     /translation="MLFRHKGILYLFLSSLIISAANPVIAKLITLGNEHLVNGRNPIS
                     FCNVLFAGNFIAVLTLILIHFRELKEFQPEHLTTKNWCLIFLAALFAGFLTPTLFFFG
                     LMYTNVINVVLISNLQIPMTLLAGWIFLGEVPNIRVTTGAFLATLGVFAIVILQYWLG
                     GAGALTPNPGMKTGPLFIFLSSIPRSGEICIFLAVVSSTASTIIGFHALRRLPHYLFS
                     ILKMLLGLVLFFFIVISMFGWHHFADLFSPFLWEWMLFYGSVIIALRYYFSMLGMKHA
                     KVAEVAISSSIIPLMSIVFSYLILGEVPGISQIIGGSLILFGIYVALTGKLQAPEKTE
                     VLEKPSGFSGV"
     gene            complement(1793..2551)
                     /locus_tag="VAH76_002930"
     CDS             complement(1793..2551)
                     /locus_tag="VAH76_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387509.1"
                     /translation="MIIYASIWLIIVMKDLIAFIHDLYKDRTEKSVKFESKGRVIVSP
                     LMSKEDARELFKSLKEILPEEARLKVRESKQEPDQFRVVLFNPENVFSFYAKRAAELL
                     NRFRPFPIFSSEDKYYKWEYNPASRLIEYRVTFSILDLDTEEKIPDPGKDVKELKVID
                     QYNKKLQSTYPGITILGNNSEDGGDDSFSLALNYSDYKAINSPPSLRDIGIEFFRKHP
                     ENFTTEVSKKIPGDMTDEFTDIKPSSTKPSSTGS"
     gene            complement(2665..3429)
                     /locus_tag="VAH76_002931"
     CDS             complement(2665..3429)
                     /locus_tag="VAH76_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1387510.1"
                     /translation="MGYSIINKNRSIKIILMIFIGITTFVKAYAYSPDTSNPIQINRS
                     LTEVNSRHPGEVIVLIHGLMRTYVSMKPLKAYLEAQGYQVYLYKYPSARYNIQEHGVY
                     LNQFITTILENNPAIKIHFVTHSLGGIIVREALSKLSQKQLKHVGYLIMLAPPNQGSK
                     LAKLSIKLFPMISYFIKPLAELSSEQTSYVHRVPVPDIKMGIIAGRFDAKVPPDYARL
                     QGQTEQVIINNTHTFIMYDSKVKKLVLSFLEKGKFE"
BASE COUNT         1252 a          671 c          586 g         1109 t
ORIGIN      
        1 tatgctaaaa aataattgaa gcaatataag gagagtacaa tgaatgtgca taccatgtgt
       61 tttacgaggg tgttacgtta tgctgttgtg acaagcattc tgtttatggc tgtgtctttt
      121 acatcaatgg caaatgcggc tcagggatgc ggggagggtt atcacagggc cattcataat
      181 ggtacttgtg ttctaaacta tccaggcccc tttgcaaccc ctgcaccagc acatccaggc
      241 tgctggcgta atatgtgggg acaattgcgt tgctatagat actaattcaa gcttgaaaat
      301 acaaacatat caaaccttat ttcttgctgt tatatttttg atgtaactag caaaaataag
      361 gttttattca tagaaaaata gtcattctaa acacctgaga aacccgaagg tttttccaag
      421 acttcggttt tctcgggagc ctgcaatttt cctgttaaag caacgtatat cccgaacaaa
      481 attaaacttc cacctatgat ttgcgaaata ccgggaactt cacctaatat caaataggaa
      541 aaaacaatag acatcagagg aattattgat gaactgattg caacctctgc aacttttgca
      601 tgtttcatgc ccagcatact gaaataatat cgcaaagcaa taataacact cccataaaag
      661 agcatccact cccataaaaa tggggaaaaa agatcagcaa aatgatgcca gccaaacata
      721 gaaatcacta taaaaaagaa taaaacaagc cccaataaca tttttaaaat actaaacaga
      781 taatggggta atcttctaag tgcatgaaaa ccaataatag ttgaagcagt actggaaact
      841 actgccaaaa aaatacaaat ttcacctgaa cgaggaatag agctgagaaa aataaaaaga
      901 ggacctgttt tcattccggg gtttggagtt aaagcgcctg ctccccccaa ccaatattga
      961 agaataacta ttgcaaatac tcccaaggta gctaaaaaag cccctgtggt tactcgaata
     1021 ttaggcacct ctcccaagaa aatccagccg gctaataaag tcatagggat ctgtagatta
     1081 gaaataagaa caacattaat cacattggtg tacattaatc caaaaaaaaa taaggtgggt
     1141 gttaaaaaac ctgcgaataa agcagccaga aaaattaaac accaattttt cgttgttaaa
     1201 tgttctggtt gaaattcttt tagctctcta aaatgaatta aaatcaaagt gagcactgca
     1261 atgaagtttc cggcaaatag gacattacaa aacgaaattg gattacgacc attgaccaaa
     1321 tgctcattcc caagagtgat aagttttgct atgacagggt tagcggcact tattatgagc
     1381 gaactcagga ataaatatag tattccctta tgtcgaaaca gcatcttaat ttccccctac
     1441 atccttatca aggttttaat tttgtacact caattttacc tgtatctaca tcttttttca
     1501 ggtgagagac agcaataaat agcatatctt gattagctcc ccctgatgca caatcaaata
     1561 gcatggatag aatatgcacg actttaataa tcttatcttt cgaatcaata cataaaattt
     1621 ctttagattc cttaagtgca gctaaaaatt tactttataa atctccctta cagcacaata
     1681 cgctgcaata agagaagaga aaagaaaatg atacaattaa atccaccttt atttacaaga
     1741 taaaaagcca cagtttctat ttatccgttg ctcctctatg ctcaataaaa aattagctgc
     1801 cggttgacga tggtttagtg gaagatggtt taatatcagt aaattcatcc gtcatatctc
     1861 ctggtatttt tttagaaacc tctgttgtaa aattctcagg gtgttttcta aaaaattcaa
     1921 tccctatatc acgcaaactc ggaggactat taatagcttt ataatctgaa taatttaaag
     1981 ccagggaaaa gctatcatcc cctccatcct ctgaattatt cccaagaatt gtgatcccag
     2041 gataagttga ctgcaacttc ttattatatt gatctatcac ttttaattct ttaacatcct
     2101 ttccaggatc aggtattttc tcttctgtat caagatcaag aattgaaaac gtaacgcggt
     2161 actctattag cctggatgcg ggattatatt cccatttgta atatttatct tctgaagaaa
     2221 atatagggaa tggtctaaat cgatttaata attcagcagc acgcttggca taaaacgaaa
     2281 aaacattttc aggattaaag agaacgacac gaaactgatc tggttcttgc tttgactcac
     2341 gtacctttaa tcgcgcctct tcagggagta tttcttttaa tgatttaaat aattcccggg
     2401 catcctcttt actcatcaaa ggagatacaa taaccctccc cttggattca aattttacag
     2461 atttctcagt acgatcctta tacaagtcat gaataaaagc tattaaatct ttcatgacaa
     2521 ttatcaacca tattgacgca tatataatca aatttagtta taattaatta acatgtcaat
     2581 ttttacacct ggatttttga caataaaagc tggatagaat acttaattaa gccaatacta
     2641 gcctctgact aaaactaaaa aaaactattc aaatttccct ttttctaaaa agctcagcac
     2701 aagttttttt acttttgaat catacataat aaaagtatgg gtattgttaa taataacctg
     2761 ctctgtctgt ccttgcagac gagcatagtc tggaggaact ttagcgtcaa accgcccagc
     2821 gattatcccc attttgatat caggtacagg aacacgatgc acataagatg tttgctcaga
     2881 acttaattca gccaaaggtt ttataaagta ggaaatcatc ggaaatagtt taatagataa
     2941 ttttgccagc ttcgaacctt gattaggagg agccagcata atgagataac ctacgtgttt
     3001 taattgtttt tgagataatt tggataacgc ttcccttaca ataatgccac caaggctatg
     3061 ggtaacgaaa tgaattttta ttgccggatt attctcaagt atcgttgtaa taaattgatt
     3121 taaataaact ccatgctctt gaatattgta tctggcggag gggtatttgt acaaatagac
     3181 ctgatatccc tgagcctcca gatatgcttt taatggcttc atactcacat aggtacgcat
     3241 taaaccatga attaatacaa taacctctcc aggatgcctg gagtttactt ctgttaatga
     3301 acggtttatc tgaattggat tactggtatc aggggagtaa gcataagcct tgacaaatgt
     3361 ggttattcca atgaatatca tcagaattat ttttatgctt cgatttttat tgataatgga
     3421 ataacccatt attatctcta ttattgattt gaataaaatt attctttcta tagataaact
     3481 taaaatgatt attaatcaag aatatccatt caattattgc atcgagttga gtgcaaacta
     3541 attcagaatg aaaataaaac cagcaaatga gcttatgatt gccttatcag ataaaataaa
     3601 ctccatattt cccaacta
//
LOCUS       DATNSN010000223         3603 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.224, whole genome shotgun sequence.
ACCESSION   DATNSN010000223 DATNSN010000000
VERSION     DATNSN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.224"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            8..1885
                     /locus_tag="VAH76_002932"
     CDS             8..1885
                     /locus_tag="VAH76_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO1387511.1"
                     /translation="MVFIMQKIIILTKKIIAKLPVILFDVAAIPVAWYAAYWLRYNMQ
                     PYPSSLTSTHSFIALALLSIVQISCYYYFKIYRGLWRFSSLNDVIRILKATITAMVLV
                     IPVFYLTSILQHLPRSVFPLYCIILATILCGGRLVIRLHWDKPGRGNRELETKRVLVV
                     GAGQAGEGLVRDLKRSSYYRPIGFVDDNKSKRGLEVHGVRVLGTTNQITELVNQYDVD
                     LIFIAIPSAKSATMRRIVTLCEQSHVPFSTLPSISALAAGRVEVNALRPVNIEDLLGR
                     DQVTLEWEKIARSIAGRRILITGGGGSIGSELCRQVMALEPASIAIVENSEFNLYRIE
                     QELLKSFPGIPVELSLISVTDEIAINHLFRRFQPEIVFHAAAYKHVPMLQHQVRVAVF
                     NNVIGTQVVAKASVTFGAEKFILISTDKAVNPTNIMGTTKRVAEIYCQNLNTRVKTQF
                     ITVRFGNVLGSAGSVVPLFQKQLQEGGPLTVTHPDMQRYFMTIPEACQLILQAMVNGE
                     GGEIFVLDMGEPVKISYLAEQMIRLAGKEPGRDIMIEYTGLRPGEKMYEELFHETEQL
                     TSTKHEKLFKARFRELDWNDLTQTMRMLQAACIEHQDEELLVLLKSLVPEFQLEPMVE
                     A"
     gene            complement(2031..2624)
                     /locus_tag="VAH76_002933"
     CDS             complement(2031..2624)
                     /locus_tag="VAH76_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387512.1"
                     /translation="MLPFKYGYMSRVDIMTRSNSEQLEEEYLNSRREYCIAMMAVHDK
                     ARALLSETPIDGSMKQAIIKSSGVKIRPNETQDEFDERFEDEMGLTIDDYVGFALDIL
                     EKKLEPARQSIVAAANKELGSLTSGPSGSAWEDFQHTIHEGAEYKTIEVAKCLPESER
                     CIQAFNQAKLAFRDAATNLIHAEKLEEPEEPNQGPRF"
     gene            complement(2991..>3603)
                     /gene="sidK"
                     /locus_tag="VAH76_002934"
     CDS             complement(2991..>3603)
                     /gene="sidK"
                     /locus_tag="VAH76_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector v-ATPase inhibitor SidK"
                     /protein_id="HEO1387513.1"
                     /translation="RLQAAIELYQLLGRTMDKQIHLFAKMNLEQINEYIQTKTKSILD
                     KIPERVELLTFMGFEIPTFKGIETLMTDISHSQDNETLAIAQEFYTNIKNAKNQLLGD
                     KLIEDITPQDVEKFFNQCSQYGSEAAEKLADNRPVLTKIADILTAIARWAISLIGFNT
                     PPQFLAPTRTCVDQVSDEITKIKLKLEDTLGSLQKAQEESLSL"
BASE COUNT          985 a          665 c          777 g         1176 t
ORIGIN      
        1 acagactgtg gtttttataa tgcaaaaaat aatcatattg acaaaaaaaa tcattgcaaa
       61 actcccagta attttgtttg atgtggccgc aataccagtt gcctggtatg ccgcttactg
      121 gttgcgctat aacatgcagc catatcccag cagcctgaca tccacccatt cttttattgc
      181 tttagcttta ttatcaattg ttcagattag ttgttactat tattttaaaa tttacagagg
      241 attatggcgt ttctcttctc tgaatgatgt catcaggata ctgaaggcta ctatcacagc
      301 aatggtttta gtaatccctg ttttttactt gacttcaatt cttcagcacc tgcccagatc
      361 agtttttcct ttgtattgca ttatcctggc taccatcctt tgcgggggaa ggttggtgat
      421 ccgtctgcat tgggataagc ctggcagagg aaacagggaa ttggaaacga aaagagtttt
      481 ggtggttggt gccgggcagg ctggtgaagg cttggtgcgt gatttgaaaa gaagcagtta
      541 ttatcggcca ataggctttg tagatgacaa taaaagcaag agaggattag aagttcatgg
      601 agtgcgggtt ctgggaacga ctaaccaaat taccgaatta gtcaatcagt acgacgtgga
      661 tttgattttc attgccatac cttctgccaa atcggcaacc atgcgaagaa ttgtaacgct
      721 ttgtgaacaa agccatgtgc cttttagtac tttacccagt atttcagccc tggctgcggg
      781 ccgggttgaa gtgaatgcgc tgaggcctgt gaatattgag gatttattgg gtcgggatca
      841 agtgacgctc gaatgggaaa aaattgctcg ttcaatagca ggaagacgca tattgattac
      901 aggcggtggt ggctcaatcg gttcggaatt gtgccgtcag gttatggctt tagagcctgc
      961 gagcatagcc attgttgaga atagtgagtt taacctgtat cgcattgaac aggagttgtt
     1021 aaagagtttt ccaggtatcc ccgttgaatt gagtttaatc agtgtgacag acgaaattgc
     1081 gattaatcac ttatttcgtc ggtttcaacc tgaaattgta tttcatgcgg ctgcttacaa
     1141 acatgttccc atgcttcagc atcaagtacg ggttgctgtg tttaataatg tcataggtac
     1201 tcaggttgtt gccaaagcca gtgtgacatt cggagctgag aaattcattt taatttccac
     1261 tgataaagca gtgaatccaa cgaatataat gggaactacc aagagagtcg ctgaaattta
     1321 ttgtcagaat ttaaatacac gagttaaaac ccagtttatt acggttcgtt ttggaaatgt
     1381 tctgggttca gctggaagtg tggtgccttt gttccaaaag cagttgcagg aaggtggtcc
     1441 actgactgtg acccatcctg atatgcaaag atattttatg actataccgg aagcatgcca
     1501 gttgattttg caagcgatgg tgaatgggga gggtggtgaa atttttgtcc tggatatggg
     1561 agagccggtt aaaatcagct atcttgcaga gcaaatgatt cgcttggcag gcaaagaacc
     1621 aggaagagac atcatgattg aatatacagg tttgcgtcct ggcgaaaaaa tgtatgaaga
     1681 gttgtttcat gagactgaac agttaacttc aacaaaacat gaaaaattat tcaaagcaag
     1741 gtttcgtgaa ctggattgga atgatttgac acaaaccatg cgaatgttac aggcagcttg
     1801 tattgagcat caggatgagg agttgcttgt tctgttaaaa agtctggtgc cggagtttca
     1861 attggaacct atggttgaag cttaatcaat atccctaatc tggtaaatca agattgtgtt
     1921 atggcaatta cttatgttga ttttgaaact ctattttctc atcatttgat ggtaaaatga
     1981 aaaacagcaa aagaattttt tagcttcttg cttcttcctg tgtcctggtt ttagaatctg
     2041 gggccttgat tcggctcttc tggttcttcc agtttctctg catgtattaa gtttgtggct
     2101 gcatcacgaa atgccagctt agcttgatta aaagcttgta tgcaacgctc agactcaggg
     2161 agacacttag ccacttctat agttttatat tctgctccct cgtggattgt gtgttgaaaa
     2221 tcttcccatg ccgaaccaga tggtccagag gtaagagaac caagttcttt atttgctgca
     2281 gctacaatac tctgtctcgc aggctccaat tttttttcta atatatcaag cgcaaaacca
     2341 acgtagtcat ctatcgttaa acccatttcg tcctcgaaac gctcatcgaa ttcgtcttgt
     2401 gtctcgttag gccggatctt taccccgctt gattttatta tagcttgctt catacttcca
     2461 tctattggag tttcactcag taatgccctt gctttatcgt gtacggccat cattgctata
     2521 caatattccc ttcttgagtt aaggtattct tcctccagtt gttcactatt tgaccttgtc
     2581 atgatatcta ccctactcat atacccatat ttaaaaggta gcatttgatt tataattatt
     2641 caaaaagaca ataataatac catcaaggtc atggtgcata ctaagtcaac atccctgatt
     2701 ttttattgaa tatttgatct taaattgagt gggtatttta atttcacttc gatctgctct
     2761 ctctcgatcc aggggcttag gtggtatgat gatgcttttc gcggaagatt gttgaggaga
     2821 gggaggggta aaatctgtgc taatcctacc agactcatta gtaggtatat gatgcataat
     2881 ctaatcgtaa agtggcctcg gattacagga aagctgtaat ccgggaggat atatctgata
     2941 acaggtaaat tcttatatca aatatttttt taatgtggac ttaatcgtta ttaaagactt
     3001 aggctttctt cctgtgcttt ttgcaagctt cccaaagtat cttcaagttt gagtttaatt
     3061 ttagtgattt catcagatac ctgatccaca catgttctgg ttggagccaa aaactgcggt
     3121 ggcgtgttga aacctattaa actaatcgcc caacgggcaa tggcagtcaa aatatcagca
     3181 atttttgtca gtaccgggcg attgtcagct aacttttcag ctgcttctga accgtattga
     3241 ctacattgat taaaaaattt ctctacatcc tgtggagtaa tgtcttcaat cagtttatca
     3301 cccaacaatt ggttcttggc attttttata tttgtataaa attcttgggc tatcgccaga
     3361 gtttcattat cctgagaatg ggagatgtcg gtcataagcg tctcaattcc cttgaaagtt
     3421 ggaatttcaa agcccataaa agttaataac tcaactcttt caggaatttt atccaggatt
     3481 gatttggttt tggtttgaat atattcatta atttgttcga gattcatctt ggcaaaaaga
     3541 tggatctgtt tatccatggt tctcccaaga agctgataca attctattgc ggcttgtagt
     3601 cga
//
LOCUS       DATNSN010000224         3602 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.225, whole genome shotgun sequence.
ACCESSION   DATNSN010000224 DATNSN010000000
VERSION     DATNSN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.225"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            135..2153
                     /gene="legC6"
                     /locus_tag="VAH76_002935"
     CDS             135..2153
                     /gene="legC6"
                     /locus_tag="VAH76_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HEO1387514.1"
                     /translation="MSHSKREQNPIARNLSDFRQVATLLNNEGIVVTEHNYKKVQALL
                     NRYKELATFLDQNQSKLNGKQIGELNQIRLIISPEERREIRKSVTRIEDGIKNREKRI
                     KEEYTRIEKQQAKAREAVQVLWPQLQEIAKKFINSGIANMEELLKEYKYLRNELYNHA
                     LNLPAQERKQKNQEMAELSRLVNIHESKLCDEINRARKVIEAAVTQAQKNVDSMSLLQ
                     MVRLMLPKLKDADILKIAQKAGVLSLQDTTTSEHGANLDKLVVRKQPLEKQSALISII
                     FKELALNGAQAGPLAKYLQAVQENAKDLISGKKTHFTLLYQTNEGEIGGFYFNIHELL
                     QNQHQAISKLVHEMYKRGVDQNSRVVMAFASPEQAASLMLANGENIKALTEAGNLQEL
                     TEQLLENAGDSKALARIVGHDVVIGQKTSEIIRDLNRSKEQNGIEPAEVMKLFQIIAV
                     IFETRNILINWITAPVHLKEQMEEALLGAGKTPAIVEGKGTQPKRVRFLPDLVEKPKA
                     TLPDTEQPVTKRKDDELLDKKIQVALEKVDQALEELKGKIGDINQHKYDKAYEAAKIL
                     LRQLQETRDQYAIDLANPQINFKEAGLKFKEASNLVIQQAKPILEKDLGWGEYLKNLF
                     KVIVNAIVFCVTFGASQGFFATTRAKSAEAVEKAESELELSQIGSLPK"
     gene            complement(2261..2818)
                     /locus_tag="VAH76_002936"
     CDS             complement(2261..2818)
                     /locus_tag="VAH76_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO1387515.1"
                     /translation="MARTLSNMLPLGTVAPHFELLDVVSGSKVKLRETPRYVATVIMF
                     ICNHCPFVKHINNELTQLANDYIPQGIRFLAINSNDVESYPDDSPENMIQTAKVHQYP
                     FPYLFDETQEVAKAYNAACTPDFFVFDSDLSLVYRGQLDDSRPGNGIPVTGNSIRQAL
                     DCLLNNKPFDFEQKPSIGCNIKWKI"
     gene            complement(2842..3342)
                     /locus_tag="VAH76_002937"
     CDS             complement(2842..3342)
                     /locus_tag="VAH76_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387516.1"
                     /translation="MSKTKYDSIYLVLGIVGALLLIYGLSQNNPQVYYITGSLALMIT
                     AIHYKLIYFIALELILGAGHTSLLLGMGRYIGLALPSLLCLQLLIFYLMFGKGNSIFL
                     LTGIIGIALLSMGLAYHNQIIFFSGSLGVAAYSYYSAYRGQYICYIWGILNTIFASYA
                     LYQLVF"
BASE COUNT         1343 a          605 c          658 g          996 t
ORIGIN      
        1 gacgcacaac ttgtattcta ttattcaatg tgcattaaga tattgtaatt ttgtaaaaaa
       61 ttgcccaatt aaatgtgaca aaatagcatt ccttgattat aatgcgagca cactaaccat
      121 caaggaatca ttctatgtct cattccaaaa gagaacaaaa tcctattgcc cggaatttaa
      181 gtgactttag gcaagttgca acactattaa ataacgaggg tattgttgtt actgaacata
      241 actataaaaa agtccaagcc ttattaaata gatataaaga gctagcaacc ttccttgatc
      301 aaaaccaaag caaattaaat ggaaaacaaa taggtgaatt aaatcaaatt agattaatta
      361 tttctccgga agagcggcga gaaatcagaa aaagtgttac acgtatcgaa gatggaataa
      421 aaaatagaga aaaaagaata aaagaagaat ataccagaat agaaaaacaa caggctaagg
      481 caagagaagc agttcaagtt ttatggccac agttgcaaga gatagccaaa aaatttataa
      541 attctggaat agcaaacatg gaagagctat taaaagaata taaatactta agaaacgagt
      601 tatataatca tgcgctcaat ctccctgctc aagaaagaaa gcaaaaaaat caagagatgg
      661 ctgagttatc tcgtcttgtt aatatacatg aaagcaaatt atgcgatgaa attaatcgag
      721 ccaggaaggt aattgaagct gccgtgactc aagcacaaaa gaatgtagac agtatgtcat
      781 tgctgcaaat ggttcgactc atgctgccta aattgaaaga cgcagatatc ttaaaaattg
      841 ctcaaaaagc tggagtactt tccttacagg atacaacaac ctctgaacat ggggcaaatt
      901 tagataaatt ggtcgtaaga aaacagccat tagaaaaaca atctgctcta atttctataa
      961 tattcaagga attggctctc aatggtgctc aggctggtcc tctggcaaaa tacctccagg
     1021 cagttcaaga aaatgcaaaa gatctaataa gtggtaaaaa aactcacttt actctgctat
     1081 atcaaacaaa tgagggtgaa attggagggt tttattttaa cattcatgaa ttgttgcaga
     1141 atcagcatca agcaatttcc aagcttgtac atgaaatgta taagagagga gtagatcaaa
     1201 atagtcgagt tgtaatggct tttgctagtc cagaacaagc tgcgtctttg atgttagcta
     1261 atggagaaaa tattaaagca ttaactgaag ccggcaattt acaagaatta actgaacaat
     1321 tattagaaaa tgctggtgat tcaaaagcat tggctcgcat agtaggacat gatgttgtaa
     1381 ttggccagaa aacttcagaa ataattagag atcttaatcg ttcaaaagag caaaatggta
     1441 tagagccagc tgaagtaatg aaattatttc aaataattgc agtaatattt gaaactcgaa
     1501 atattttaat caattggata actgctcctg tgcatttgaa ggagcaaatg gaggaggctt
     1561 tattgggcgc aggtaaaact cctgccatcg ttgagggcaa aggaacccaa cccaaacgcg
     1621 taagattcct acctgattta gtggaaaagc caaaagctac attgccagat actgaacaac
     1681 ccgttaccaa aagaaaagat gatgaactac ttgataagaa aatacaagtt gcattagaaa
     1741 aggttgatca agcattagaa gaactgaaag gtaaaatagg ggatatcaat caacataaat
     1801 atgacaaagc ctatgaagca gcaaaaattc ttctaagaca attgcaagaa acacgtgatc
     1861 aatatgctat tgatttagct aatcctcaaa tcaatttcaa agaggcaggg cttaaattta
     1921 aagaagctag taacctggtg attcaacagg ccaaacctat cctggaaaag gatttaggtt
     1981 ggggagaata cctcaagaac ttgtttaaag tgatagtaaa cgctattgta ttttgtgtga
     2041 cttttggagc aagtcaaggc ttctttgcta cgacacgtgc gaaatctgct gaagcagtgg
     2101 agaaagcgga gagcgaactg gaactaagtc aaatcggttc attaccaaaa taatacatat
     2161 ggttaactag tttgttatga gagcaacata cgatttttat tatacatata atgagtaatg
     2221 gaaatcgatg ttgctttttt ggattaaaga tagcttcagc ctatattttc cacttgatgt
     2281 tgcatccaat acttggtttt tgttcaaaat caaaaggttt attatttaat aagcaatcca
     2341 aagcctggcg tattgagttg ccagttacag gaatcccatt tccaggtctt gaatcatcta
     2401 attgccctcg ataaactaaa gacaaatcag aatcaaaaac aaagaaatct ggtgtgcaag
     2461 ctgcattata tgcttttgct acttcctggg tttcatcaaa aagatatgga aatggatact
     2521 gatgaacttt agcagtttga atcatatttt caggtgagtc atcagggtaa ctctctacat
     2581 cattggagtt aatagctaaa aaacgaattc cttggggaat atagtcatta gcaagttgtg
     2641 ttaattcatt attaatatgt ttcacaaagg gacaatggtt gcatataaac ataatcacag
     2701 tagccacata acgtggagtc tctcttaatt taactttact tccactaacc acatccaata
     2761 attcaaaatg aggagctact gtacctaatg gaagcatgtt tgatagagtt ctagccattt
     2821 tattaaatcc ctcaaataat cttaaaatac caattgataa agagcatatg acgcaaatat
     2881 tgtattaaga atgccccata tgtaacagat gtattgacct ctatacgcac tataataaga
     2941 atatgcagct acgcctaaac ttccagaaaa aaatatgatt tgattatggt aggcaagccc
     3001 catcgaaagc agtgctattc ctataatgcc ggttaataaa aaaatactgt ttcccttgcc
     3061 aaacattaaa taaaaaatca gcagctgtaa acacagcaaa gaaggtaaag ctaatccaat
     3121 atatctcccc ataccaagaa gtagggaggt atggcctgcc cccaaaatta attcgagagc
     3181 aataaaataa atcaatttat agtgaattgc tgtaatcatg agagcaagtg agccagtaat
     3241 gtaatagact tggggattat tctggctcag cccataaata agtaacaaag caccaacaat
     3301 acctagtacc agatatatcg agtcatactt tgttttactc acggatcacc attttaggta
     3361 aattccttta gcaaccgatt cctgtataac ttcattttgc gatttattag tagaaatcca
     3421 atagtttccc atattactaa gtgcaaattt aacacgagga tattggcaga cttcgagaac
     3481 atctcgacaa tcaactaaag cagtattttc atcaacatgc ttataacact taaagtttaa
     3541 attttttaca tctgacgcga atgctgccca actcattgga tctagcgatg cttgtaaggg
     3601 ag
//
LOCUS       DATNSN010000225         3589 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.226, whole genome shotgun sequence.
ACCESSION   DATNSN010000225 DATNSN010000000
VERSION     DATNSN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.226"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            277..918
                     /locus_tag="VAH76_002938"
                     /pseudo
     CDS             277..918
                     /locus_tag="VAH76_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            976..1509
                     /locus_tag="VAH76_002939"
     CDS             976..1509
                     /locus_tag="VAH76_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387517.1"
                     /translation="MSFDFIKSIWIAALIGITPTLSYSESNIQYQETLQSHCFETWMK
                     KMDPSIDKETYKQFGDKYCRCISTKNLDNKTGVQKAIRNCIPQTILHDAMDELEEEVT
                     LSEVTTQTIEQYCLNRWSLIYPNQSDEDKKTIQAYCACVKTKLLSFIEQIEKISNKEY
                     NEAINDINTICSENLNQ"
     gene            1595..2518
                     /locus_tag="VAH76_002940"
     CDS             1595..2518
                     /locus_tag="VAH76_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO1387518.1"
                     /translation="MSSKNTYNTVLNKQEINWILEQRITRQTDNKEVLIYPMGAERYL
                     SSALSGHEEERYNLHSEIKEKNVLIIPGYGNSAFLFAQAGAKSILVYDKDPVTIAWVK
                     AFKKYYHYREYNNSGKPYPSIGELLTALTKWYPPLLKLPSGVIKNSVFWIVNPKLLRK
                     SYIYYMLKLTRQAVQLRVQNEFEFDHHIEFYAGELKHLIKNHPSKTFDTVFVPYLLGV
                     QNGIEKEKDIVSFIKQLIELVPYGHILVTPSKSTKEFHLVGQSYFCTTAYANIQSIPD
                     LKPYIIAEDSIWFKTQGLAVFGIKPGAENRL"
     gene            complement(2653..>3589)
                     /locus_tag="VAH76_002941"
     CDS             complement(2653..>3589)
                     /locus_tag="VAH76_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEO1387519.1"
                     /translation="SLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNALAN
                     ELKTDFYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEKLI
                     LQFEDALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQALAH
                     SPDVIERVKKLIRHSDFDLSNPNKVYALIGSFIKNPFGFHSVTGEGYQLVADAILDLD
                     EINPTLAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVKED
                     LPLPIHLTFHGGSTMQDRTTQLIADGNKENAYQLY"
BASE COUNT         1285 a          618 c          572 g         1114 t
ORIGIN      
        1 ctttaaaata atcagcgcta tgaatccttt gcctatttga gatattcttt ttcaattatt
       61 tttccaaaca ttaaaaaacg catctccctt ggagcttaag aaaaatctca agatccaggt
      121 aaaaaacgtt ttttaggagg gaatttagat aaactaaatg attgaattat aaatattttt
      181 taacaaagag attgagtttt tttgtgagtc ctgtttttgc aaaaagttaa aagatcaatt
      241 attttgattt tacaagccac ggatagagaa tttaaattga agactctcgt cataacagga
      301 atcagtagag ggataggcct ggaaattgct agaattttcc ttgacaaaga ctggacggtt
      361 attggtactt ccaccagcgg atattctcct ataaaacata aaaacctgcg cacatacaag
      421 ttagacctag ccatttcgga gcacatcgat gcatttgcaa aaaatttacc taaaattgat
      481 gtactgatta ataatgccgc cgtgttgctt gaagagtgga atgaagaaaa aattaatatg
      541 tccttattga aaaaaacctt tgcaattaac ctgtttggta cagttgagtt aactgaaaaa
      601 tgtatcccca agttcaatga tggtgcacaa atcattaata tctcttcagg atggggagca
      661 tttagttcca ataattcccc ctttcaacca cattataaaa tgtcaaaagt cagtttgaat
      721 atgtatacta aattgcttgc agagagactt ccccaaataa ctgtatccag ttttgatcca
      781 ggttgagtta aaacaaacat gggaacacaa aatgcaaaaa aacttcccag tgaaacggca
      841 ctggaaatct atgagctgat tagtatgaag aaaaaaagcg gctatttttg gtttaatgga
      901 aaacctaggg attggtagct agttaaattt cagttattct caactataaa ataaagaatc
      961 aaaggagtga caaacgtgag tttcgatttt ataaaatcaa tatggattgc agctcttata
     1021 ggtataacac caaccttgtc ttattcagag tccaatattc agtatcaaga aacacttcaa
     1081 tcacattgct ttgaaacctg gatgaaaaaa atggatccat cgattgacaa agaaacatat
     1141 aaacaatttg gagataaata ttgtcgatgc atttcaacta aaaatctcga taataaaaca
     1201 ggggttcaaa aagcaattag aaattgcata ccacaaacaa ttttacatga tgccatggat
     1261 gaactggagg aagaggtgac tttatcagaa gtaacgacac aaacaatcga acaatattgt
     1321 cttaatagat ggagccttat ttatcctaac caaagtgatg aagataaaaa aacaatccag
     1381 gcttattgtg cgtgcgtcaa aacaaaatta ttgagtttta tagagcaaat agaaaaaata
     1441 tcaaacaagg aatacaacga agccattaat gatattaata cgatttgctc ggaaaattta
     1501 aatcaataac atagtgtaat ataattaaat gttgactaaa taaataatca tatttagcac
     1561 cagccagctc taatgcttta tgaaaggcct attcatgtca agtaaaaata cctataacac
     1621 tgtactcaat aagcaagaaa taaactggat tttagagcaa cgtattacaa gacagactga
     1681 caataaagaa gttttaattt atccaatggg agcagaacgc tatttgtcat ctgctttatc
     1741 tggtcatgaa gaagaaaggt ataatttaca cagtgaaatc aaagaaaaaa atgtcctaat
     1801 tatccctgga tatggtaata gcgctttttt atttgctcaa gcaggggcca aatctattct
     1861 tgtttatgac aaagatcccg tgactattgc atgggtaaaa gcatttaaaa aatattacca
     1921 ttacagagaa tacaataata gtggaaaacc atatccttca ataggtgaac ttctgacagc
     1981 cctcaccaaa tggtatcctc ctctacttaa actaccttca ggagtgatta aaaactctgt
     2041 attttggata gttaacccta aattactaag aaaatcatat atttattata tgctgaaact
     2101 caccaggcaa gctgtacaat taagagttca aaatgaattt gagttcgatc atcatataga
     2161 gttttatgca ggtgaattaa aacatctgat aaaaaatcat ccgtcaaaaa catttgatac
     2221 agtatttgtt ccttacctct taggtgtaca aaatggaata gaaaaagaga aagatattgt
     2281 tagttttatt aagcaattaa tagagcttgt accctatggt catattttag tgaccccttc
     2341 aaaaagcact aaagaattcc acctggttgg gcaaagttac ttttgtacaa cagcttatgc
     2401 taatatacag tccattcctg atttaaaacc ttatatcatt gcagaagaca gtatttggtt
     2461 taaaactcaa ggacttgcag tatttggtat aaaaccaggt gcagagaatc gtttgtgaaa
     2521 ataaggctat gatttattgc attagaaaac gtgcttcctc attacctacc aaggaagggg
     2581 agtattcacc ccccaagttt taggcaatat caatatctcg cgcttttcta aatcaggtta
     2641 tgaaataaga aatcaataca attgataagc attttcctta tttccatcag caattaattg
     2701 agttgttcta tcctgcatgg ttgatccccc atgaaaagtc aaatggattg gtaatgggag
     2761 atcttctttt accttatcca atcccttttt tgccattgtt ctcacatcag atgaagtagc
     2821 attagagtag attattttta aagtgctaat catcatagct tgcctattta catcgtattt
     2881 atcccaataa gtaaattttt cagttagatt ggcagccaaa gttggattaa tttcatccaa
     2941 gtccaaaatg gcatcagcaa ccagttggta accttcacct gtaacagaat gaaatccaaa
     3001 tgggtttttt ataaatgatc ctatcagagc atataccttg ttaggattag acaaatcaaa
     3061 atcactatga cggattaatt tttttacccg ctctatcaca tcaggggagt gtgccaatgc
     3121 ttgaatactg aaccagttat ttacagctcc cggatcattt ttccaataat gataataatc
     3181 ctctaatgca acatccgctt cctcacaatt tatttcgcat aacatgctta aagccaaagc
     3241 agtctctgtc atatttttgc ctaaagcatc ctcaaattga agaatcagtt tctcctttgt
     3301 tttttcaaaa tcaacattta gcaaataaga aaaacatacg gattttaaac gccgcaatcc
     3361 cgcttgcttc aaatcaaaac cttttaattt ttgtttatca tcatccccag atatttgtat
     3421 tctaaaaagg agattataaa aatccgtttt caattcattt gcaagggcat tttgtattaa
     3481 ttgacgtccc tcatttaatt tttcaaaatc aggcttatcc tgattttcaa tcaactcctc
     3541 taaagacggt aaggtaatta attcagctag catccattca tttaaagag
//
LOCUS       DATNSN010000226         3535 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.227, whole genome shotgun sequence.
ACCESSION   DATNSN010000226 DATNSN010000000
VERSION     DATNSN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.227"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..850)
                     /locus_tag="VAH76_002942"
     CDS             complement(<1..850)
                     /locus_tag="VAH76_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEO1387520.1"
                     /translation="MIVIFVHGWSVTHTNTYGELPQWLENQSKQGKLDIQVGNIYLGR
                     YISFDDTVTVDDIARAFDQAVRDEIADKLRDGQRFACITHSTGGPIVRKWMDLYFKNN
                     LAKCPLSHLIMLAPANHGSALAQLGKSRLGRIKSFFEGIEPGKCVLDWLELGSDMSWQ
                     LNESWLDYDCTANGVYSFVLTGQKIDRQFYDAVNSYTGESGSDGVVRVAATNMNYSLL
                     KLHQEGDNGESLVVAKMTRTQPMAFGVLPGLSHSGKNIGIIRSITMANAATHPTAIWI
                     LRCLQVK"
     gene            1326..2372
                     /locus_tag="VAH76_002943"
                     /pseudo
     CDS             1326..2372
                     /locus_tag="VAH76_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2455..>3535
                     /locus_tag="VAH76_002944"
     CDS             2455..>3535
                     /locus_tag="VAH76_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO1387521.1"
                     /translation="MPRKNLILNLPGFSIVKVSGYQPLLLDVSYNRLARCGHCQSKKV
                     RKKSSYLREVHHELIGHRRSILRFKAYKLYCHDCGRYGNQQFPGINKHQRATWRAQAA
                     VFHEHSRGVSQKDLSERYKKGKATIERWYQRHYEEQNRELLNRPCPIVLGIDEHFFSK
                     KEGFATTFCDLRKHKVFDVVRGRREKELKEYLQQLPGKERVKVICMDLSSTYRSLVKK
                     YFPNAMIVADRFHVIRLIQHQCMMTCRELSSEIKNNRGILALLRTRPDNLSDEKKVKR
                     DTFFTENPAIESIYQFQQQLHSLLMKRALTQHECRKVIPTFLDMLSELKQSGFKALAS
                     LGRTLCAWKDEVARMWRFSKSNGITE"
BASE COUNT         1100 a          646 c          734 g         1055 t
ORIGIN      
        1 tttttacttg caggcatcgc aggatccata tcgccgtggg gtgagtggct gcattggcca
       61 tagtaatact acggatgata ccgatatttt taccggaatg tgaaagtcca ggtagaactc
      121 caaatgccat tggctgtgtt cgtgtcattt tggcgacaac aagactttca ccgttatccc
      181 cctcctgatg taatttcaat aagctataat tcatatttgt tgcagcaacg cgtaccaccc
      241 catcggatcc agattctcct gtgtaggagt ttactgcatc ataaaattgg cgatcaattt
      301 tctggccagt aagtacaaag gaatagacac cattggcagt gcaatcataa tcaagccagc
      361 tttcattaag ttgccagctc atatcgcttc cgagctcaag ccaatcaagt acacactttc
      421 ccggttcaat gccttcaaaa aaactcttta tgcggcctag gcgggatttg ccaagctgag
      481 caagagcaga accgtggttg gcaggagcca gcatgataag gtgactcaac ggacattttg
      541 caagattatt cttaaagtat aaatccatcc atttgcggac aatgggccca ccggtagagt
      601 gagtgataca agcaaaacgc tgcccatctc gtagcttatc agcaatttca tcacgtaccg
      661 cttgatcaaa tgcgcgtgct atatcgtcaa ccgtcaccgt atcatcaaaa ctgatgtagc
      721 gcccaagata aatattgccg acttgaatat ccagctttcc ctgtttgctc tgattttcaa
      781 gccattgagg aagttcccca taagtattgg tatgcgtgac actccagcca tggacgaaga
      841 taacaatcat aacgctcctt gaatcgttgt ctcacaacaa gacattgttg agtaacttga
      901 tgatattaga ttattttata ctcaatcctc aagcttttaa ttatggctcc agaattttca
      961 gtgttaaaaa ataagctgcc ttttaatgaa tattttgcct actgttaaaa gacaagagct
     1021 atataagttt gaatgatatc tttttgaaat atttttcatc aattttattc taattaatta
     1081 actgaaagga aatcgattgt ttatatatga cttatacaaa atttctagtt gattcaacga
     1141 ctgcttatgc ggcttagaag ttttgaaaat tatagtccat aagatattat ggatatgaat
     1201 gggtgatata gtacaatatt gcacggcgag aaagattggg acagattaag gtgaaaagac
     1261 gtagcttttg taaatcagct ggaatccgct ttttttagcc ttgcaaagta atataagaag
     1321 catccatgct tgttgaactg tttaattcat ccattaaaaa aatcattgtc aaattgaacc
     1381 aactaaaagg cttgtggatt gtccaactca atttgtcaaa aaaccgactg agtgagaaaa
     1441 aattcactga cttggcagtt ctgctcagta tgctcaaggc ttttggagtc acttatctta
     1501 atttgcattt caataatctt gggcaaaaga ctggaactga attggtagcc ctgtttaaga
     1561 ctcttaagga ctctggaatc agccgtctta gtttaagtgc taatgagctt tatcaaaaga
     1621 ccgggacgga gttagccgcc ttgttaactg ttttaagcga ttcagatatc attcagcttg
     1681 atttaagctg caattggctg ggacaaagac cagacgctga actaatcgaa ctatttaagg
     1741 catggagtag tactccaata attgatcttg acttgaccaa taatgatctt ggtaaaaaat
     1801 cggggatcgt gttgtcggaa atgttccgtg cattgaaagt atcttcaatt agccatcttg
     1861 gtttacgtaa taatgatctt tatcaaagga caggtaccga attttcatcc ctattgagtg
     1921 ccataaaaga ctctccaatc acccacctcg atttaagtga taatggattg catctaatgg
     1981 ttgttactgc ttttgagacc ctatttgata ctctaaaaga ctcctccgtc acccatctca
     2041 atttatgccg taatggcatt gggtatctgg acatgttaga aatgacagcg atgttttatt
     2101 cactccagtg tacaggggtg actcatcttg atttaggcga gaatagtttg gagttgatga
     2161 ccgctgagga gttagcagct atattccgag tctttaaaga ttccagatta attcatcttg
     2221 gtttaaatga tagctattta aataacagaa caacagaaga tttgcatctt atttttaatg
     2281 cattgccagc caatatagcg acgattaaat tatcgcttga ggagttaact gagatgagca
     2341 gcgagcaacg tcaggcgatt caaaggcgtt ttggctctac accaattaag ggggcacttt
     2401 aatcaaatta gtctagacta aatccttgat tttgcgatca aaaaggagat taaagtgcct
     2461 aggaagaatc ttatcctaaa tttacctgga ttttctatag taaaagtgag tggataccag
     2521 ccgttgttat tagatgtttc ttataatcga ttagcgcgtt gtggtcattg tcaaagtaag
     2581 aaggtacgca agaaatcatc gtatctgcga gaagtacatc atgagttgat aggtcatagg
     2641 cgaagtatct taaggtttaa agcttataag ttatattgtc atgattgtgg tcgatatggg
     2701 aatcaacagt ttcccggcat taataagcat caacgtgcta catggcgtgc gcaggcagca
     2761 gtatttcatg agcatagtcg aggagtatca caaaaagacc tgtcggaacg ttataagaaa
     2821 gggaaagcga caatagaacg ctggtatcaa cggcattatg aagagcaaaa tagggaatta
     2881 ctcaatcgtc catgcccgat agtactcggt attgatgagc atttctttag taaaaaagaa
     2941 ggctttgcta caacgttctg tgacctaaga aaacataagg tatttgatgt ggttcgtggc
     3001 cgtcgtgaga aagaattaaa agaatatctc cagcaattac ctggaaaaga gcgtgttaaa
     3061 gtgatttgta tggacttaag tagtacgtac cgttccttag taaagaagta ctttccaaat
     3121 gcgatgatag tggctgatag gtttcatgta atccgtttaa ttcaacacca gtgtatgatg
     3181 acctgtcgag aactctccag tgaaattaaa aacaatagag gtatcttagc cttattgaga
     3241 acgagacctg ataacttaag tgatgagaag aaagtcaaaa gagatacatt tttcactgaa
     3301 aatccggcaa tagaatccat ttatcaattt cagcaacaac tgcactcact attaatgaaa
     3361 agggcgttaa cgcagcatga gtgtcgtaaa gtaataccta cttttttaga tatgctttct
     3421 gagttaaagc aaagtggatt taaagcgtta gcctcattag gcagaacgct ctgtgcttgg
     3481 aaagatgagg tcgccagaat gtggcggttt agtaaatcta atggaataac tgaag
//
LOCUS       DATNSN010000227         3523 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.228, whole genome shotgun sequence.
ACCESSION   DATNSN010000227 DATNSN010000000
VERSION     DATNSN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.228"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..395)
                     /locus_tag="VAH76_002945"
     CDS             complement(<1..395)
                     /locus_tag="VAH76_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO1387522.1"
                     /translation="MIDKFWMQLAYEQAVIARNEGEVPVGAVLVSKDNQLLGVGRNVI
                     EKSHDPSDHAEIRAIRQASRKLNNHRLLDTTLYVTLEPCVMCAGLMVHARIKRLVFAT
                     RDFKAGAAGSMFNVLHALNHSVLIDEGIM"
     gene            complement(392..1969)
                     /gene="guaA"
                     /locus_tag="VAH76_002946"
     CDS             complement(392..1969)
                     /gene="guaA"
                     /locus_tag="VAH76_002946"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO1387523.1"
                     /translation="MNDLKKSPLLILDFGSQYTQLIARRVREMGVYCEIYPYHINHEQ
                     FKKLNPCGVILSGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVHSS
                     ALREFGYAELRLHGHSQLLSNIEDRTAVDGSALLDVWMSHGDKVTELPPGFKVICETR
                     NAPIAGMADESRQMYGLQFHPEVTHTLQGLRILQRFVVDICKAPTEWTPEHIIDEAIN
                     KIREQVGTEKVLLGLSGGVDSSVVAALLHRAIGEQLVCVFVDTGLLRLNEAEQVLSMF
                     GRHMGIRIIAVNAEDKFLTALKGVTCPEEKRKIIGRTFIEVFDEEAQKLTDIKWLAQG
                     TIYPDVIESAATSTNDAAVVIKSHHNVGGLPDTLNLKLLEPIRELFKDEVRQVGLELG
                     LPHDMVYRHPFPGPGLGVRILAEVKKEYADILRKADAIFIEELHNAQLYHKISQAFAV
                     FLPVKSVGVMGDGRRYDYVICLRAVETVDFMTAHWSQLPWDFLGKVSNRIINEVEGVS
                     RVTYDISGKPPATIEWE"
     gene            complement(1972..3444)
                     /gene="guaB"
                     /locus_tag="VAH76_002947"
     CDS             complement(1972..3444)
                     /gene="guaB"
                     /locus_tag="VAH76_002947"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO1387524.1"
                     /translation="MPLSIVQQSLTFDDVLLVPAHSTILPKDVSLKTKLTRAIHLNMP
                     LISAAMDTVTEARLAIALAQEGGLGIIHKNMSIADQAEEVRKVKKFESGMVKDPISVT
                     PDLTVKELLAVMTKYNFSGVPVVDGKHLVGIVTSRDIRFETNMNLTVAQVMTPKGRLV
                     TVKEGASREEVRSLLHKHRIEKLLVVNEDFELRGLITVKDIQKAKDNPYACKDSFEQL
                     RVGAAVGVGEGTDERVAALVESGVDVIVVDTAHGHSQGVLNRVKWIKKNYPDVQVIGG
                     NIATAAAARDLYEAGADAVKVGIGPGSICTTRIVTGVGIPQISAIANVAQELKGIIPV
                     IADGGIRFSGDVCKALAAGADTVMLGSMFAGTEESPGEIELYQGRTYKNYRGMGSIGA
                     MSLAQGSSDRYFQDASLGTEKLVPEGIEGRVPYKGPVQTIIHQLLGGLRSCMGYTGCA
                     TIEELHSKTEFVQVTNAGMRESHVHDVSITKQAPNYQVDN"
BASE COUNT         1030 a          853 c          583 g         1057 t
ORIGIN      
        1 tgcataattc cttcgtctat taataccgaa tggtttagag catgtaaaac attaaacatt
       61 gagccagctg cccctgcttt aaaatctcgg gtagcaaata ccagccgttt gatgcgtgcg
      121 tgaaccataa gtcccgcaca catcacacag ggttcaagag taacataaag cgttgtatcc
      181 aataatctat gattatttaa ttttctcgag gcttgtcgta ttgccctgat ttcagcatga
      241 tcagaaggat catgactttt ttcaatgaca tttctgccaa cgcctaataa ttgattgtct
      301 ttgctaacca acacagcccc aacaggaacc tctccttcat tacgagctat aaccgcttgc
      361 tcgtaagcca gttgcatcca aaatttatca atcattccca ttcaatggta gcaggtggct
      421 tgccagaaat atcgtaagtg acacgtgata ctccctctac ttcattaata attcgattgg
      481 atacttttcc taaaaaatcc cagggcaatt gagaccaatg tgctgtcata aaatcaactg
      541 tctcaacagc tcttaaacaa attacgtaat cgtatcggcg tccatccccc atcacaccca
      601 cgctctttac tggtaaaaaa acagcaaatg cctggctgat tttatgataa agctgtgcgt
      661 tatgcagttc ttcaataaaa atagcatcgg ctttacgtaa tatgtccgca tattcttttt
      721 ttacttccgc taaaattctt acacccaatc caggtccagg aaagggatgc cgatagacca
      781 tatcatgcgg gagtcccaat tccagaccta cttgacgtac ttcatcttta aagagctcac
      841 gaataggttc cagcagttta agatttaatg tatctggtaa gccgccgaca ttatgatgag
      901 actttatgac cacagccgca tcattagtac ttgttgctgc agactcgatg acatcggggt
      961 agattgtgcc ctgagccagc cactttatat cagtcagctt ctgtgcttcc tcatcaaaga
     1021 cttcgataaa tgtacgccca ataatttttc tcttttcctc tgggcaagtt actcccttca
     1081 aagcagttaa aaatttatct tcggcattga ctgcaataat gcgtattccc atatgccgac
     1141 cgaacatgct cagcacttgc tccgcctcat taagtctgag taaacccgta tcaacgaaaa
     1201 cacaaaccag ttgctctcca atggctctat gaagtaatgc ggctacaaca gaagagtcaa
     1261 cgcctccgga tagtcccagt aaaacttttt cagttcctac ctgttctctg attttattaa
     1321 tggcttcatc aataatatgc tctggagtcc actctgttgg tgctttacaa atatctacga
     1381 caaaacgttg taaaattctt aaaccttgca aggtatgagt aacttctgga tgaaattgca
     1441 aaccatacat ttgacgactt tcatcagcca tacctgcaat tggtgcatta cgtgtttcac
     1501 atatcacttt aaaacctggc ggtaactcag tgactttatc accatggctc atccatacat
     1561 ccagtagagc actgccatcc accgcggtac gatcttcgat attacttaat aattgactat
     1621 ggccatgcaa acgcaactca gcatatccaa attctcttaa cgccgatgaa tgcacttggc
     1681 caccgagttg tacagccatg gtttgcatcc catagcatat acccagcaat ggtaaatcgc
     1741 tctcaaacag ccattgagga gctctgggat tagcatcatg cgttactgtg gaaggacctc
     1801 ctgataaaat cacaccacaa ggatttaatt ttttaaattg ttcatgatta atatgataag
     1861 gataaatttc acaataaacc cccatttctc gaacgcgtct tgcaattaat tgggtgtatt
     1921 gagaaccaaa atctaatatt aataatgggc tttttttcaa atcattcatt attaattatc
     1981 cacctgataa ttgggggctt gcttagtaat actcacatca tgcacatgtg actcacgcat
     2041 accagcattg gtcacttgca caaattcagt tttagaatgc aattcctcaa tagtagcaca
     2101 accggtatag cccatacagg agcgtaaacc acctaataat tgatgaataa ttgtttgaac
     2161 cggtccttta taaggtactc tcccctcaat accttcagga accagctttt ctgtaccaag
     2221 agaagcatcc tgaaaatagc ggtcacttga cccctgagct aatgacatgg ctccaatgga
     2281 tcccatacct ctgtaatttt tataagtacg cccctgatat aattcaattt caccaggaga
     2341 ttcttccgta cctgcaaaca tactaccaag cattacagta tcagcacctg cggccaacgc
     2401 tttacaaacg tcacctgaaa aacgtatccc accatcagct atcacaggaa ttatcccttt
     2461 aagttcttgt gccacatttg ctatagcgct aatttgcggg ataccaacac cggtcactat
     2521 gcgagtggta caaattgatc ctggcccaat tccaactttt actgcatcag cacccgcctc
     2581 atataaatct cttgcggcgg cagcggttgc aatattacca cctatgactt gtacatcagg
     2641 ataattcttt ttaatccatt tcacgcgatt taatacgccc tgagaatgtc catgtgctgt
     2701 atccactact atgacatcaa ctccggattc aacaagagct gcaactcgct catcggtccc
     2761 ttcaccaaca ccaacagcag ccccaactct tagctgctca aaactatctt tacaagcata
     2821 agggttgtct tttgcttttt gtatgtcttt tactgtaatg agtccacgta actcaaaatc
     2881 ctcattcact actaataatt tttctattcg gtgcttatgg agcaaacttc tgacttcttc
     2941 tctacttgct ccttctttta cagtaaccaa acgccctttg ggagtcatta cttgcgccac
     3001 agtcaaattc atattggttt caaaacgaat atctcgactg gttactattc ctaccaaatg
     3061 cttaccatca accaccggca cccctgaaaa attatatttt gtcatgactg caagcaattc
     3121 tttaactgtc aagtcgggag ttactgaaat aggatctttc accataccgc tttcaaattt
     3181 ttttactttt ctgacttctt ctgcttgatc agcgatactc atattcttat gaataatccc
     3241 caaaccacct tcctgtgcta aagcgatagc taacctcgct tcggtcactg tatccattgc
     3301 tgccgaaatt aaaggcatat taaggtggat tgcacgggtt aatttggttt tcaatgaaac
     3361 gtctttgggt aaaatagttg agtgggcagg gacaagcaaa acatcgtcaa aggttaatga
     3421 ctgttgcaca atggatagag gcatttttat ctcactccag taattaaata accaaatagt
     3481 ttactacaaa ctttaaaaat tttaaacatt taatgtataa att
//
LOCUS       DATNSN010000228         3520 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.229, whole genome shotgun sequence.
ACCESSION   DATNSN010000228 DATNSN010000000
VERSION     DATNSN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.229"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(519..1796)
                     /locus_tag="VAH76_002948"
     CDS             complement(519..1796)
                     /locus_tag="VAH76_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEO1387525.1"
                     /translation="MSLPEMFALIDCNNFYASCERLFRPDLKDVPIVVLSNNDGCCIA
                     RSNEAKALGIAMGEPYFKIKHLCKQHGVKAFSSNYTLYGNMSHRVMCTIEEAWPHIEI
                     YSIDEAFLDLRSLPVNSHNLFCEQLQKKILKYTGIPTSIGIGPTKTLAKAANHLCKKV
                     YKIPVFNITSSRERLLQQISVGDIWGVGRQWANKLISRGIHTAYDLAMTNPHLLKKCF
                     NVVLMRTAMELQGIACGGLEAIEPKQSIMSSKSFGQMQTQLASIEESISSHCARAVEK
                     MRRQQLVAKRMVVFVNTNRFREDLAQHFQSIEFKLINPTDDLRLITKIAKRCLQRIFK
                     PGYYYKKAGVWLEDLIPKSPRQLDMFHQPSDEHLKHTEQLMGVFDQINQKYGRSTIRL
                     AAEGYSKPWAMRAELKSPAYTTRWSEVPQVRLF"
     gene            complement(1783..2289)
                     /gene="umuD"
                     /locus_tag="VAH76_002949"
     CDS             complement(1783..2289)
                     /gene="umuD"
                     /locus_tag="VAH76_002949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEO1387526.1"
                     /translation="MSPRGGKREGAGRPRGEPTKAVRIPLSQLAKLERLKSHESYQLP
                     VFASKIQAGFPSPADDYIEGYLDLNTKFIKHPSSTFVLQATGESMVDAGIFSGDWLLV
                     DRSIEPSDGRIVIAAVNGELTVKRLSKKGGRVQLLPANPKFQPIDITEDSEMVIWGVV
                     TLVLHELA"
     gene            complement(2425..3093)
                     /locus_tag="VAH76_002950"
     CDS             complement(2425..3093)
                     /locus_tag="VAH76_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEO1387527.1"
                     /translation="MCGRFAYIASYDKLKYQFHLANAIEIPPRFNISPGADVVCLVET
                     VGHEIQCVLLRWGLIPSWTTDRKKLGNLINARAETVFEKPTFRQSIKSKRCLIPMSGF
                     YEWRQEDGVKQPYFFQKKNHDLLAVAAIRDIWQQNEEVIHSCCLITTDANAFMQPVHN
                     RMPVILGEEAQAIWLNNTQCDKAQLMALMKPYPYEDLEGYRVTTLVNKANFDHPLAME
                     PLSE"
BASE COUNT         1088 a          752 c          624 g         1056 t
ORIGIN      
        1 ttggaaaagc agctttgctt gctcctgcac ctagcagaac aacatgaggc tttaaatcct
       61 taggattttc aattaattct tgtttcgtaa ccattagacc aaccaaaata agataagtta
      121 aataaagtct aaattagtca aaccttaaaa tttcttatat aaatatttaa gatgattttc
      181 atcgtaccta ttaacatata acatgttcat taactcttac acaaacaaag ataatgaact
      241 ttgatttctg taaagagttt acaaacagtt tttaagcata tattggcggc tcactcttac
      301 cctatttatt cgtccgtaaa cccatcgttt tatggaaata agtttctaat gttctaactt
      361 gtttttccat atacccccat gagtacagag catatgatga tgcggcgata taccttcaat
      421 gcttgataga agttagcatg gcggggcgta ccccaaatgc ctgtgctgcc taattccaag
      481 ttgtaataac ccttcctaaa ctagcctccc tctcctattc aaaacaaacg aacctgcgga
      541 acctcagacc atcgtgtagt ataggcaggc gatttcagtt cagcacgcat cgcccaaggt
      601 tttgaataac cctctgctgc gaggcgaatt gtacttcgtc cgtatttttg attgatttgg
      661 tcaaagaccc ccatcaattg ttcggtgtgt tttagatgct catcacttgg ttgatgaaac
      721 atatccagct gtcgtgggct tttaggaatt aaatcttcaa gccatactcc tgccttttta
      781 tagtaatacc ctggtttaaa aatacgctgc agacatcgtt tggctatttt agtaattaag
      841 cgcaaatcat ctgtgggatt aatcagctta aattcgatgg actgaaagtg ctgtgccaaa
      901 tcttcccgaa atcggttcgt attaacaaat acaaccatgc gcttcgccac caattgctgg
      961 cgacgcattt tctccaccgc acgggcacaa tggctactga ttgattcctc aatcgaagca
     1021 agttgggttt gcatctgacc aaaactttta gatgacataa tactttgctt aggctctatt
     1081 gcctctaaac cgccacaagc aattccttga agctccatgg cggtacgcat caacacgacg
     1141 ttaaaacatt tcttcagaag gtgaggattg gtcattgcca aatcataagc cgtatgaata
     1201 cctcgcgaaa ttaatttatt ggcccattgc cgtcctactc cccaaatgtc tccaacggaa
     1261 atctgttgta ataaccgctc acgactcgag gtgatattaa acacagggat tttataaact
     1321 tttttgcata aatgattggc ggctttggct agtgttttgg tcggcccaat gccgatggaa
     1381 gtgggtattc ccgtgtactt caagattttc ttttgtaact gctcgcaaaa caaattatgc
     1441 gagttaaccg gtaaactcct taaatcaaga aacgcttcat caatcgagta aatttcgata
     1501 tgaggccagg cttcttcaat agtgcacatc acgcgatgac tcatgttacc gtacaacgta
     1561 taatttgagg aaaaagcttt cacaccatgc tgtttgcaca aatgtttaat tttgaagtat
     1621 ggctcgccca tggcaatgcc caatgctttg gcttcattcg agcgtgcgat acaacagcca
     1681 tcgttattgg atagcaccac gatggggaca tcctttaaat caggacgaaa caaacgctca
     1741 caactggcgt aaaaattgtt gcaatcgata agggcaaaca tctcaggcaa gctcatgtag
     1801 aaccaaagtc accactcccc aaatcaccat ctcactgtcc tctgtaatgt caatgggttg
     1861 aaatttggga ttagcaggaa gtaattgcac ccttccccct tttttcgaaa ggcgttttac
     1921 cgtgagttca ccgttcacag ctgcaatgac aatgcgccca tccgaaggtt ctatgcttct
     1981 atcgactaac agccagtcgc cagaaaaaat tccagcatcc accatagact cccctgtggc
     2041 ttgcaaaaca aaggtagatg atggatgttt aataaatttg gtatttaaat caagataccc
     2101 ctcgatgtaa tcatcggccg gtgatggaaa tcctgcttga attttactgg caaataccgg
     2161 caattgatat gattcgtgac tttttaaacg ctctaatttt gcaagttggg ataatggtat
     2221 acgaactgct ttggtaggct caccccttgg gcgacctgcc ccttctcttt ttccacctcg
     2281 tggtgacatg atgattcttt ttttgattta cgtacaaata tcatagacaa ggaaaaataa
     2341 gctgtaaatc ccgcctcaga acagcatgag ttgaaatcaa cactaagtct tcgagaatat
     2401 taggaaaatt atttttaaat ttttttattc agataatggc tccatcgcca agggatggtc
     2461 aaaattggct ttgttcacta aagtagtcac tcgatagcct tccaaatcct cataaggata
     2521 aggtttcatt aacgccatta attgtgcttt atcacattgc gtgttgttaa gccaaatagc
     2581 ttgagcctct tcacctaaaa ttactggcat acgattgtgc actggttgca taaatgcatt
     2641 agcatccgtt gtaatcaggc aacaggaatg aataacctct tcattctgct gccagatatc
     2701 ccgaatagca gcaacagcca gcaaatcatg attctttttt tgaaaaaaat aaggctgttt
     2761 tacaccatcc tcttggcgcc attcataaaa gccactcata gggattaaac atcgtttcga
     2821 ttttattgac tgacgaaaag tcggtttttc aaaaactgtt tcggctcgtg cattgatgag
     2881 atttcctaat ttttttctgt ccgttgtcca ggaaggaatc aatccccaac gcaataatac
     2941 gcattgaatt tcatgaccaa cggtttcaac aagacatact acatcagctc ctggagaaat
     3001 attaaagcgc ggaggtattt caatcgcatt ggctagatga aattggtatt ttagcttgtc
     3061 ataagaagca atataagcaa atcgaccaca catcttttca tccaccgaaa gagttattgt
     3121 cagtataata ctaaatctca aataatgaga aaaatcaggc gaaatattca ttcattaaaa
     3181 caatatttgc aacacgagca atgatatcca aatttagcac cctctttagt tcagaaaact
     3241 gtattaaccc ggctaaccat ttttatatta actcaaaatt tctaaagatg agtaacgaca
     3301 tggatatcga caaagatatc atcacagaga ccattacagg tatcacaaaa agaatcataa
     3361 tagatatatt tataggtacc atatggatat ttatatagat acccatatga aaacatataa
     3421 gatatcaaca aagataacat aatgatatat aattcgttat cttagtttat cacgcctata
     3481 ttttatatag gtcacagcgc aaaaggcaaa tatacagatt
//
LOCUS       DATNSN010000229         3462 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.230, whole genome shotgun sequence.
ACCESSION   DATNSN010000229 DATNSN010000000
VERSION     DATNSN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.230"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            249..2018
                     /locus_tag="VAH76_002951"
     CDS             249..2018
                     /locus_tag="VAH76_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO1387528.1"
                     /translation="MNQEISNFSTFNFSSALNKALEDMKFITPSPIQAQTIPLILQGR
                     DAIALAQTGTGKTAAFALPILQNLSPEISTTQALILAPTRELAIQVAEQFELLSKYQR
                     NVTIAVLCGGQEYGRQLKQLRSGAQVVVGTPGRILDHIDKGTLLLNNLKTFILDEADE
                     MLRMGFIEDVETILEKLPEKKQMALFSATMPYRIRQIANTYLNDPASIEIRMETATVK
                     SIEQRFLFASVHQKPDALIRVLEVEDYQGVIVFVRTKSSTEEVAELLQQHGLRAMAIH
                     GDITQSLRERIITQFKQGAIDILVATDVAARGLDVERVTHVINYDMPHDNETYVHRIG
                     RTGRAGRSGVTILFVTPKESRLISSIERHTRQRIEKVQVPNDHMIQMARQQRFMSNIT
                     TRLNHEHVYSYKRIVEEYIKEHDVSAVDVAAALALLLHKDMPWRKELNMPKAVSAFKE
                     ERARAGEKGLDLRGRFKEDRKSSFADRKGARGSKKDFAKDFSNERKKFNDGRKAVNQE
                     SVAQDLFRIDVGKIHGVKPGNIVGAIANEAGLQSRYITGLKIHEDHSTVRLPKGMPKE
                     VMKDLTKAWVCGRQLNLTLIGSA"
     gene            2368..2586
                     /locus_tag="VAH76_002952"
     CDS             2368..2586
                     /locus_tag="VAH76_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387529.1"
                     /translation="MKKKHEPGFFGRETKNIKDIESNRYDPDCPIRRADKSRGFSQKA
                     NSQFQLRIKEEVKEALDDIRNESAYKFN"
     gene            complement(2746..3372)
                     /gene="mavE"
                     /locus_tag="VAH76_002953"
     CDS             complement(2746..3372)
                     /gene="mavE"
                     /locus_tag="VAH76_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavE"
                     /protein_id="HEO1387530.1"
                     /translation="MTRFIMLSFVTGYRKSISCNRFSRNVVSPYFFMRTIIMTRFERN
                     FLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQINSRITNADDWSKEDKLKFTS
                     AIAEAIALVSEKYENPTSETTEQIQSARNILLDDYVPLLTANTDPENRLKSVRENSSQ
                     IRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAATAASVLFGNKP"
BASE COUNT         1076 a          600 c          728 g         1058 t
ORIGIN      
        1 tgtcatcctg cgcaggcggg aagccatttt tattgtaagc tgtagagcaa aatttgaaat
       61 agattttcac ttgcgtggga atgacacaca tgatgtatta tttagtcaaa atatgatttt
      121 gccctggttg ttgtcttgat aaaattaata aatatagtgc atcatcttgt taattttgtt
      181 attataatgc acatttttta tattagttag ccttttcaga aagacgcttt attatattca
      241 ggaacagtat gaatcaagaa atatcaaatt tctctacttt taatttttct agtgctttaa
      301 ataaagcgtt agaagatatg aagtttatta cgccatcgcc tatccaggct caaaccattc
      361 ctttgatatt gcaaggtcgg gatgccattg ctcttgcgca aactggtaca ggcaaaacag
      421 cggcttttgc attaccaatt ttgcaaaatt tgtctcctga gatatccacc actcaggctt
      481 tgattttagc ccctactcgt gaattggcaa tccaggttgc agagcaattt gaattattaa
      541 gcaagtacca acgcaatgtg acgatcgccg ttctttgcgg tgggcaagaa tatggtcgtc
      601 aattaaaaca attgcgtagt ggtgcgcaag tggttgttgg aacgccagga cgtattctgg
      661 accatatcga taaaggcacc ttgctcttaa ataatctgaa gacatttatt cttgatgaag
      721 cagatgaaat gttgcgtatg ggtttcattg aagatgtaga aaccatttta gaaaaactgc
      781 ctgaaaaaaa gcaaatggca ttgttttcag caaccatgcc ctatcgaatt cgtcaaatag
      841 ccaatactta tttgaatgac cctgcctcaa ttgaaatccg tatggaaact gcgacggtca
      901 agtctattga gcaacggttt ttatttgctt ccgtacatca aaaaccggat gcgttaatcc
      961 gggtcttgga agtggaggat tatcaagggg ttatcgtttt tgtacgcact aaaagcagta
     1021 ctgaggaagt agcagaacta ttgcaacaac atggattaag agcaatggca attcatggtg
     1081 atattactca gtctttgcgt gagcggatta ttacccaatt taaacaagga gcgattgata
     1141 tccttgtggc gacggatgtc gctgcaagag gtttggatgt cgagcgagtt actcatgtga
     1201 ttaattatga tatgccacat gacaatgaaa cttatgttca tagaattggc agaacaggga
     1261 gagctggccg ttctggtgtt acgattttat ttgtaacccc caaggaatcg cgtttaattt
     1321 ccagcattga acgccatacc aggcaacgaa ttgagaaagt tcaagttcct aatgaccata
     1381 tgatacagat ggctcgtcaa caaagattca tgtctaacat tacgactcga ctgaatcatg
     1441 agcatgtgta ttcctacaaa aggattgtgg aagaatacat taaagagcat gacgtatcag
     1501 cagtagatgt tgcggctgct cttgctttat tgctgcataa agatatgcct tggagaaaag
     1561 aattaaatat gcctaaagca gtttctgcat tcaaagaaga gagagctcgg gcaggagaaa
     1621 aaggattgga tttacgtggt cgatttaaag aagatagaaa aagttctttt gctgatcgga
     1681 aaggtgcgcg aggaagcaag aaagattttg ccaaagactt ttctaatgaa agaaaaaaat
     1741 ttaatgatgg caggaaggcc gttaatcaag aatcagtagc tcaggattta tttcgtatcg
     1801 atgtaggcaa aattcatgga gtcaaacctg gcaacatagt aggggctatt gctaatgagg
     1861 ctggcttgca aagccgatat attactggtc ttaaaattca tgaagatcac tctactgttc
     1921 gtttaccaaa gggtatgcct aaagaagtga tgaaggactt aaccaaggct tgggtttgcg
     1981 gccgccaact caatttaaca ttaattggta gtgcctgatt agctcagcag taaagtgatg
     2041 ttagcgttat caattggcct ttaattattt tgcaaaacga tttcgcctgt ccagggcgag
     2101 atcataacat caagtacaat aaatagcgca aacaatggtt cacattttat tatcccctca
     2161 caggcaggaa gctatctctt ctctatagca aagggagggg caagatttga aatagattcc
     2221 tactcgcgta ggaatgatac gcatgatgta ttatttaatc aaagtatgat tttaccctga
     2281 agacctacct tttcctgact tgtttttcta atttaatacc tctaatatgg attggtgttc
     2341 tgaaatttaa cactgagggg ccaaattatg aaaaagaaac atgaacccgg ttttttcggt
     2401 agagaaacca agaatatcaa agacattgaa agcaatagat acgatcctga ttgcccaatt
     2461 cggagagctg ataagtctcg tggattcagc caaaaagcaa acagccagtt tcagctacga
     2521 ataaaagaag aagtaaaaga agcgttagac gacattcgta atgaatctgc ctataaattt
     2581 aattaaccat tattttatct ctgagtgtaa gagatcagct cctgcttgaa acgaccgtat
     2641 ttggtccatc ttgaacggaa ataaccattg gagtactgat gatctgtata tttccgagtt
     2701 taatgaagcc tgacaagagg cttcattagt aataaaacac tacctttatg gtttgttgcc
     2761 aaataacact gacgcagcag tagctgctac ggctacagtg gcagcaacga atgggaaaag
     2821 aacatagggg ttttcaaact gacttttata aggtacttcg tctttgagtt tggcaattaa
     2881 ttcttttctt atctgactac tattttctct cacagatttt aatctattct cagggtcggt
     2941 gttcgctgta agtaatggga catagtcatc caataatata tttcgggcac tttgtatttg
     3001 ctctgtagtc tcactggttg gattttcata cttctccgaa acaagagcta tggcttctgc
     3061 gattgcagaa gtgaatttta gcttatcctc tttggaccag tcatcagcat ttgttatacg
     3121 tgaattaatt tgatagcggg ggttttcata ttggccttga gtaaaatggt gaattgcatc
     3181 atccagcttc ttgtccacag agagtatggt ttcaagaaac atcaagctat taatcaggaa
     3241 atttctttca aatctagtca ttattatggt tctcataaag aaataagggc tgacgacatt
     3301 tctgctgaat cgattgcaag atattgattt tctgtaccct gttacaaaag aaagcattat
     3361 gaatcgagtc agggaagtgt ttacaggatt tactataatt aggtttctgt tttagatcaa
     3421 caaaaaattt atatacagta atgtgttaaa tgccgtggaa aa
//
LOCUS       DATNSN010000230         3432 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.231, whole genome shotgun sequence.
ACCESSION   DATNSN010000230 DATNSN010000000
VERSION     DATNSN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.231"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            80..376
                     /gene="gatC"
                     /locus_tag="VAH76_002954"
     CDS             80..376
                     /gene="gatC"
                     /locus_tag="VAH76_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO1387531.1"
                     /translation="MTLSAKDLENISKLAYLNEDSEHSAKLTQEVSAIMDFVDQLKTI
                     DTSQIAPLFHPLALNQRLRPDEVTEAECLAELEAMAPLFEDNLYLVPKVISSDK"
     gene            379..1830
                     /gene="gatA"
                     /locus_tag="VAH76_002955"
     CDS             379..1830
                     /gene="gatA"
                     /locus_tag="VAH76_002955"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO1387532.1"
                     /translation="MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLD
                     EDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDAT
                     IVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
                     NLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAED
                     LAMILHCIAGFDSKDSTSVDRVIPDYSAEIKKPVDKIRIGLPSCFFQPQVEKGIQDAI
                     HDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRYDGIRFGHRSKSAST
                     LIELITNSRSEGFGNEVKRRILTGTHVLSTGFFNAYYLHAQKVRRLIRDELITTLNSV
                     DVIIGPTTPTTAFKLGEKINDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQ
                     LMGKHFSESRLLAIAHHYQQHTNWHLANPNKQG"
     gene            1834..3267
                     /gene="gatB"
                     /locus_tag="VAH76_002956"
     CDS             1834..3267
                     /gene="gatB"
                     /locus_tag="VAH76_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO1387533.1"
                     /translation="MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGL
                     PGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTN
                     GYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
                     HSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLN
                     SFRFIEKAIAFEQARHQDILESGLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDP
                     DLLPIHIDKEQIEAIKNNLPDLPEAISKELKNTPSLNDEDINFILSSPDTYQYYKKIK
                     SLCPAADKTIINWLKGQYAAFLNEHNLTFETPPISAKTMAAFLSKIHEKKISSSIAKN
                     IFSMLCVGEEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPDQVTEYKAGKEKLLAF
                     FIGQAMKQTKGKANPEQINLLLKKHLG"
BASE COUNT         1146 a          677 c          627 g          982 t
ORIGIN      
        1 actaaaagat aattaaacta atacttcatt tttagaacaa taccgctata atgtataacc
       61 cttatatttg gatatgacca tgactctctc tgctaaagat cttgaaaata tttcaaaatt
      121 ggcttattta aatgaagact ctgagcattc tgcaaaattg actcaagaag taagtgcgat
      181 catggatttt gttgaccagc tcaaaacaat agatacaagc caaatagcac ctctttttca
      241 tcccttggca ctaaatcaac gcttaagacc agatgaagta actgaagcgg aatgtcttgc
      301 tgaattagaa gccatggcac cattatttga ggataactta tatttggttc ccaaggtgat
      361 atcatcggat aaataataat ggaacattac tcactggctc aattatcaaa ggctcttcat
      421 aatcgagaat tttcaagtgt tgaattaaca caacactgta ttaacaaaat tcaaagcaat
      481 aaagacttga atgcattcat cagccttgat gaagaccaag ctcttaaaga agcgcaatcg
      541 gctgatttgg tactaaaaaa tggagaggga aagccgctga ccgggattcc catggctctc
      601 aaggatttgt tttgtacaaa gcgattaaat acaacttgtg cttcaaaaat gctggctaat
      661 tttcaagccc cctatgatgc cactatcgtc acaaaattta aacaaaatgg cgcaataatt
      721 attggaaaaa ccaatatgga tgagtttgcc atgggatcct ctaatgaaaa cagttacttc
      781 ggttcggtaa aaaacccgtg ggatagagaa agagtgccag gtggatcatc aggcggttct
      841 gcagcagcgg ttgctggaaa tctggtacct tttgcaattg gctctgacac cggtggatca
      901 attcgtcagc cagcagcctt ttgcgggatt agcgggataa aacccaccta tggattagtc
      961 tctcgatacg gaatggtcgc ctttgcctca agccttgatc aagcgggacc atttgcgaag
     1021 tcagctgaag atctggccat gatacttcac tgtattgcgg ggtttgatag taaagattcc
     1081 acatcagttg atagagttat acctgattat tcagcagaaa ttaaaaaacc agtagacaaa
     1141 atacgcatag gacttccttc ttgctttttt caacctcaag tcgaaaaagg tatccaggat
     1201 gccatacatg atgctgtgaa attatttgag aatcttgggg ctgaaataat tgaaatcgac
     1261 ttgaaacttc agccactttg ggtgccatgt tactatgtta tagcttgcgc agaagcttca
     1321 tcaaatcttt cgcgttatga tggcataaga tttggccacc gaagcaaatc cgcatcaacc
     1381 ttaatcgaat taatcactaa ctctcgaagt gaaggttttg gtaacgaagt aaaacgtcga
     1441 attctaactg gaactcatgt gctctcaacc ggttttttta atgcctatta tttgcatgca
     1501 caaaaagtga ggcgtttgat tcgagatgag ttaattacaa ctttaaattc agtagatgtt
     1561 atcattgggc caacgacccc taccaccgct tttaaattgg gtgaaaaaat aaatgatccc
     1621 atacaaaatt atttggcaga tgtatttact gtagcagcca atcttgcagg cctaccagct
     1681 atttctatac caactggttt tgaaaataaa ctaccaattg gtttgcaatt aatgggtaag
     1741 cactttagtg aaagccgctt gcttgcaatc gcacatcatt atcagcaaca caccaactgg
     1801 catttagcca atcctaacaa gcaagggtga tatatggaat gggataccgt cattggtctt
     1861 gaggtacatg ctcaattaaa aaccaaatca aaattatttt caggagcatc tactgctttt
     1921 ggtgccaccc ctaactcgca aacgagtttt atcgatgctg gtttgcctgg tgttctccct
     1981 gtattaaacg aacaagccat cataatggct atccaatttg gtttggcaat tcatggaacc
     2041 attaatgatc tatctgtttt tgagcgtaaa aattattttt atcctgactt acccaaaggc
     2101 tatcaaatca gtcagtatca aaaacccatt gttactaatg gttatttaaa tatacagctt
     2161 ggtaataacc tggaaaaaac agtccatatt gcacgcgctc atttagaaga agatgctggc
     2221 aaatccctac acgatgccca tactgattac accgggatcg acctcaacag agcaggtacc
     2281 ccactattgg aaatagtcac aaccccttgc ctgcactccg ctgaagaggc gattaattat
     2341 ttaaaaacgc tgcatcaatt agtgagattc cttggaatat gcgatggcaa tatgcaagaa
     2401 ggttcttttc ggtgtgacgt caatctgtct ataaaaccta aaggcagctc agttttaggc
     2461 acacgaacag agcttaagaa tctgaattca tttcgtttta ttgaaaaagc cattgctttt
     2521 gaacaggcaa gacaccaaga tatactggaa agcggtctgt cagtcatcca agagactcgc
     2581 ctttacaatc ctgacaataa cacaactcag gcaatgagag gcaaggaaaa tgaaaatgat
     2641 taccgttatt ttcctgatcc tgatttatta ccaattcata ttgacaaaga acaaattgaa
     2701 gcgattaaaa ataacctgcc ggacttgcct gaagcaattt ccaaagagtt aaaaaacact
     2761 ccgtccctaa atgatgaaga cattaatttc atattatcat caccggacac ttatcaatat
     2821 tacaaaaaaa ttaaatccct ttgtccggct gctgataaaa caattatcaa ctggttaaaa
     2881 ggccaatatg ccgcctttct taatgaacac aacctaacct ttgaaacacc accaatttca
     2941 gctaaaacca tggctgcttt ccttagcaaa attcatgaga aaaaaatctc ttccagtatt
     3001 gctaaaaata ttttcagtat gctttgtgta ggtgaagaag atatcgatgc gattattgaa
     3061 cgcgaaggct accagcaaca gaatgacaac tcggcactgg aagaaatagt tgagcaaata
     3121 atcaaacaat atcccgacca agttacagaa tacaaagccg gtaaagagaa attattagct
     3181 ttttttatcg ggcaagcgat gaaacaaact aaagggaaag caaatcccga gcaaatcaat
     3241 ctgttattaa aaaaacatct tggatgatgc aatattttta taattcacaa tgatcattta
     3301 tggctttgtt gattgattgc cagctattat catctttttg tcagaaagta taatgcatta
     3361 agaataataa tctttcaatt atagaagaat attggaacca attttcattt agaattaatt
     3421 gcatatatga ct
//
LOCUS       DATNSN010000231         3410 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.232, whole genome shotgun sequence.
ACCESSION   DATNSN010000231 DATNSN010000000
VERSION     DATNSN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.232"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..3124
                     /locus_tag="VAH76_002957"
                     /pseudo
     CDS             158..3124
                     /locus_tag="VAH76_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
BASE COUNT         1277 a          521 c          597 g         1015 t
ORIGIN      
        1 gacatcactt gattctacag gtgtttagtt taatcactat cattaaacgg gtatatcgtg
       61 attttctggt tttttgtaaa ccaaggaaat agacgatccc gtaattcctt ggttttgaaa
      121 actttattac tagaaatttc agttaaatac aactgatttg ctgagatttc attcagattc
      181 aggggcaaaa atgattaaaa aattgttaat taaagtcgaa aaattaatta ataatcttcc
      241 aagtgagttg aaattatctg aaacgatttt atcctctatt aaagaagata taaacagcct
      301 ggcaagtcgt tttgatgggt caaaaggtta tattaattat tattctccaa aaatcgtaca
      361 acaaatcaat gacaaacaaa agttacttga gaaataccaa aattatttaa ctgcattact
      421 cgctgtttta atttgttcca agcaaagcag agacattaga gacaataaaa aacttgaacc
      481 tgtttctgag attaccgatt tgaaaaattt attaaaacac aaggatgtcg aaaaaattat
      541 caacgatatt actcagtttg atgaagacca aagagaactt aagagagttg aacgtttgat
      601 acaaaaagat gaagagaaag aggataataa aaaaatgcat cttggtaaaa gatccaaatt
      661 agagcttagg agggcagaac tttctaaaag agttcaattg tttagtaata agttaaaaaa
      721 tgttccacaa gcagaggaaa tcaaagccgt tttagattat tgtcaaaaat acatcgagaa
      781 acatatcaac cttagcaagc aattaccaaa aggaatgctt gatgttaaac gagcattgtc
      841 tttatttgac gagataaatt ccaccaagac ccaaattagg aggttaagca aagacaaact
      901 ggattcttca gggcaaataa aacttgaagg tttaaaaaag gaactcacaa aggctgaatt
      961 ggctttgcaa tttagttatt cagaggaaga agataaaacg ctcaaagaca tgctcaatga
     1021 tttgaaaagg cttatgaaaa tgagcatcag agattatcaa aagttagcca catctggcgg
     1081 agaaataaat gtttttttgc ataagacaac tggtttttat tgggaaagcc tttttctgta
     1141 cagaaatgtt ttgtgcgaaa ttaatgaata tcttaaaaaa aataaaaaga aattgtctga
     1201 acaaggaatg ctggtagctg aatgcatttc taaagccttc agccagatgc ctgaattgga
     1261 taccattgaa aaagctttag aaaaagtcaa aagaatagat gatgatctta aaagacaaga
     1321 agaatataac gaaaaaatca atggaacttg gattaggttt tataaaaaag gtccagtaaa
     1381 atttgttcaa caagtccttg aacagaactt aaatgagaaa attttttaca taccgtataa
     1441 tccgcataat tatgtgctgg atcatcctgc ccaatctgat ttcgccgata ccttaggaaa
     1501 ttgctatggc gaaactcaga tgtttctcaa acaaataaac agtaaatctc ctgcaattaa
     1561 taatatttgt cctgagaggg tattaatcaa ttatcaacta gatcaatcca ggacaattgc
     1621 taaaaataca aaaaaagaaa aaatagggat atttgaggca gatgagaaga caaaagcaca
     1681 agtaacatgg gatggaatta aagatatctt aacatcaaaa gttacctcta ccgcttttgg
     1741 tgatgtgtgc atgatcactt tgatgggagc tcaagtttct ggtcgtgaaa aattagtagg
     1801 tcatggtatt ggccttatca aaatgaagaa tcctgctcct tataaatatg tggtatatga
     1861 ttatgctttt ggtggagcaa tgggattcag tagtgatact caattggagc tattctttaa
     1921 acaaatcctt gaaggagagg gatcctatgt tccttttaag aaatgtctgg ttgaaaaagt
     1981 aggagaggtg agtaaagaat gtcaacaact tattaatggt aatcctggta ttgcttctct
     2041 gacacaaaaa agtcttgata aaacatgtaa tagagatttc tgggataaag cccgcattgg
     2101 gcttttgata aaatatcacc caattggtac agatgaaaac aggatacttg atttaataga
     2161 aaagttacct ctatctaagg atagattaga gctatatgga cagcttatta agaaccctaa
     2221 aattggtatc ggtaatcttg ttaagtgcat catttgctca ggtaagataa gttacctggc
     2281 aaatctttta acatccgatt tattaaccgt tgatattaca aataaaataa aatttaagtt
     2341 gaatgaacaa gctaaaaaag aaatagtggc tttaattaat aatgatcaga ttgaaccaaa
     2401 cttaacttgt aaactattct ctaacaatca aacgattgtt ttggcagcct ataaaaaaga
     2461 caaaaactcg ctccaatatg cggacataga ccaggttgta acgcttttgc aatgtaagaa
     2521 taccataaca ccttcggatg cctgtcgggc ttttcctaag gcagaaaaaa tagtcatcgc
     2581 agcctataag tccgatccat catcgttgca atttgctaat ctcggtttgg ttcaacaatt
     2641 attacgaaag ggttctatta ctcccgagca tgcttctaaa gcttttacag agaactcaca
     2701 attccataaa gcccatgagg catatcagtt cctcaaatct ctgaagccta tcttaaaaca
     2761 tgaaaatgaa actgagaaag ccaagagagc cgagttatta aaatggattg atcaaaagct
     2821 ggaaattact aatttgagac aagaacttta tcccttaatt taaaatatgt gtattctatt
     2881 acttgatgat attcgaatcc tttcccctga agatacaata atcaaaaaca gcagattgtc
     2941 caagttaatc agttcgaatc agtgtgatat caatatatta aaaacacttg aaaataaaaa
     3001 aatggaatta agtaaacaat gttctctgac aaaatcaaat tttacttttt ttggagctgc
     3061 agataataaa gttgatggag gaaatgctaa cactcctggc catatcactc cagagaataa
     3121 ataaaagcgt tttgttggaa ttggtcgtat agatgcggcc aatttgttaa attgaattag
     3181 tttcggctta atctgacaca actgcttgaa cacttgagcg cttccggttt tggggcattt
     3241 tatgttcggg aatttattca aagagttttt tacctctagc taactttaat taccgaaaca
     3301 acctatcaaa ttaaacatat caataaaatc ttctattaat cccgaacact atctttccca
     3361 agcaatgatt tatataaatc aattgaattt attaaggtca atatgattga
//
LOCUS       DATNSN010000232         3367 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.233, whole genome shotgun sequence.
ACCESSION   DATNSN010000232 DATNSN010000000
VERSION     DATNSN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.233"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(137..829)
                     /gene="hda"
                     /locus_tag="VAH76_002958"
     CDS             complement(137..829)
                     /gene="hda"
                     /locus_tag="VAH76_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEO1387534.1"
                     /translation="MNKQLALAIKLNDEATLDDFNWGNNTLLQQQLIEMLSFKADRLL
                     YLWGPKGSGKSHLLQACCQAINLTQSAIYLPLAFLKEWGPQSIEGLEDQTLICIDDIN
                     TIANDSAWEEALFHLYNKIKDSERSLLIISGNQPPIKCDIKLADLRSRLSWGLVIQLN
                     ELNDDEKINTLKMRAAKRGFELPESVGHFLLNRCSRNMHDLYELLNQLDDASWEAHRK
                     ITIPFVKNILKI"
     gene            complement(826..1386)
                     /locus_tag="VAH76_002959"
     CDS             complement(826..1386)
                     /locus_tag="VAH76_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEO1387535.1"
                     /translation="MILKHIPNTLTLLRLVLIAPFLMYLFHHEYVKAFYIFIAAGLTD
                     GLDGWLARHFQWQSFFGSFVDPLADKLLVASSFISLALLNSLPWWLVLLVFLRDFTIS
                     MGVLAWYYFIQRKLDFEPTRLSKLNTGFQLTLVTLCLFELAYFRFPYDLVDILIYLTA
                     FTTVTSYLDYVWTWSRKAWSPKESLK"
     gene            complement(1646..3367)
                     /gene="frgA"
                     /locus_tag="VAH76_002960"
     CDS             complement(1646..3367)
                     /gene="frgA"
                     /locus_tag="VAH76_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthetic protein FrgA"
                     /protein_id="HEO1387536.1"
                     /translation="MAYGNFHELSHQLRFLLFEIGIGLPQNSVDYFITLAHKNTLNRL
                     QHASIKEGLIQSVIASHHVHDFIDQLQIKLKNSMPESKFFQWRKIREALDESIANEAL
                     AYAYRQNWNTQLKNEAMHYKSLWTWINNELSPYQTLLFLEQWGSLRHPYHPAFRAKTG
                     FTRREVLQNSPEFQAKVSVHWCALNKTKIQSISPTIDYANQISQEFPKEYFYWREKLL
                     FSHINPDDYYPIPVHPWQWRNQLQMAFASLIDNKSLILLPHHQTLIPSLSPDIMMPTQ
                     STQCTLKLATTLSTSMAGKLDNSDDMVLLTKWIDSLLAKTNYYQNTLFICKNLESMSA
                     YDQTLSECNRVKLLFGLYQNPLHKIRQDQRAVPLPALLTDSPCSNTPLLIEIIKASEL
                     HPTTYFTEYCYKMLFGQLHLLLKYGLALEVEQHNILVIFDDNKPQGIIIKEPNNLKLC
                     NHELFKNVQKPSAPDSLSIYTKDLNQVRTLFIQGTLKNHLHHLIGCLRNEYQIPSRTL
                     WGLTRQVMQTVFKDLSKDIDPRILSWQQHLLLHDNWEHQPELLLSLHSKLNRNITIKE
                     YNPLSEI"
BASE COUNT         1026 a          554 c          683 g         1104 t
ORIGIN      
        1 aacaattgtc gtatctccat gaaacggtca tctcagtaac tctgtggata ggcaatatat
       61 ggtaaatgat aaaagaatta cggtggttcc tcatcgagga tcttaaataa atttataaag
      121 gagagtcaat aacctcttat attttcaaaa tatttttaac aaaaggtatg gtgattttac
      181 gatgagcctc ccaggatgca tcatctagct ggttgaggag ctcatataaa tcatgcatgt
      241 ttcgagagca acggtttaat aaaaaatgtc cgacactctc aggaagttca aatccgcgtt
      301 ttgctgcgcg cattttgagt gtgtttattt tttcgtcatc gtttaattca ttcaactgaa
      361 taactaatcc ccaacttagt ctggagcgta aatccgcaag tttaatgtca cattttattg
      421 ggggttggtt acctgatata attaaaagcg aacgttcgga gtccttaatc ttattatata
      481 aatggaataa agcttcttcc caggccgaat cgttagcgat ggtattaata tcatcgatac
      541 aaattaaagt ttgatcttct aatccttcaa tgctttgtgg tccccattct tttaaaaaag
      601 ctaaaggaag gtaaatggct gattgggtta aattaatggc ttggcaacaa gcttgcagta
      661 aatgtgattt gccgcttcct ttagggcccc aaagatagag caacctgtct gccttaaaag
      721 aaagcatttc aattaattgc tgctgcaaca aagtattatt accccaattg aagtcgtcca
      781 gggttgcttc atcattcaat tttattgcca gggccagctg tttattcatt ttaacgattc
      841 tttgggggac caggcttttc tgctccaggt ccaaacataa tcaaggtagg aagtaacggt
      901 ggtaaaggca gttaagtaga tgagtatatc aactaaatca taggggaatc taaagtaagc
      961 taattcaaat aaacataaag tgactaaagt taactggaag ccggtattga gtttactgag
     1021 tcgagtgggc tcaaaatcca gtttgcgctg tataaagtaa taccaggcta atactcccat
     1081 tgaaatagtg aagtctctta aaaaaacaag aagcactaac caccagggca aggagtttaa
     1141 gagagctaaa gaaatgaagc tggaggcaac taacaattta tctgcaagag ggtcaacaaa
     1201 tgagccaaaa aaactttgcc attgaaaatg cctggctaac caaccatcca aaccatcagt
     1261 taatcctgcg gcgataaata tataaaaggc cttaacgtac tcatgatgaa ataaatacat
     1321 tagaaaagga gctattaaaa ctaatcttag caatgttaag gtattgggaa tatgttttag
     1381 tatcatctgt tatacgaaaa ttagttcctc ctcttttttt atttatcgct gaggagggaa
     1441 tatattgtgc caacaatact ctattatttg agtgtttcaa atggcgtatg tgaattagtt
     1501 tatacaatac cattgtctct gtcaccttat tgagaaatca attgggattt attgactcat
     1561 agtatccacg tcccggcctc gagaagttct catattttaa gattggaaca agagcgcatg
     1621 gggtttttcg taaatccagt agagcttaga tctctgataa tgggttgtat tcctttattg
     1681 taatatttcg attgagtttg gaatgtagac ttaataacaa ttcaggttga tgctcccagt
     1741 tatcatgaag caatagatgt tgttgccaac ttagaatacg cggatcaatg tctttggata
     1801 agtctttaaa tacagtttgc atgacttggc gagttaatcc ccataaggtt cttgaaggaa
     1861 tctgatactc attacgtaaa cagccaatca agtgatgtag atgatttttt aatgttccct
     1921 ggatgaaaag ggttctaacc tgattaagat cttttgtata gatggataga gagtctggag
     1981 cgctgggttt ttgaacgttt ttaaacagtt catgattgca tagcttaagg ttgtttggct
     2041 cttttataat tatcccctga ggtttattat catcgaagat gactaaaata ttgtgttgct
     2101 ccacttctag tgctaatcca tattttagca atagatgcaa ttgtccaaat aacatcttat
     2161 aacaatattc agtgaaataa gtcgttgggt gcagttcgct agctttaata atttcaataa
     2221 gtaatggtgt attgctgcaa ggtgaatcag ttaatagggc cggtaatgga actgctcttt
     2281 gatcctgtct tattttgtgg agtgggtttt gatataagcc aaacaataat tttactcgat
     2341 tgcattcaga gagagtttga tcataggcgc tcatgctctc caggttttta catataaaca
     2401 aggtattttg gtaatagttt gtttttgcta atagggaatc gatccatttg gtaagtaata
     2461 ccatgtcatc agaattatcg agttttccag ccattgaggt gcttaaagtg gtagccagtt
     2521 taagcgtaca ttgagtggat tgtgttggca tcataatatc aggtgataaa gaaggtatta
     2581 gtgtttggtg atgaggtaac aagatgaggg atttattatc aattaaagaa gcaaacgcca
     2641 tttgtaattg attcctccac tgccaagggt gaacaggaat tggataataa tcatcagggt
     2701 tgatgtgact aaataacaat ttttcacgcc aataaaaata ttctttggga aattcttgag
     2761 aaatttggtt ggcataatca attgttgggc ttattgactg aatttttgtt ttatttaatg
     2821 cacaccaatg tacactgact ttggcctgga attcgggaga gttttggagt acttctcttc
     2881 gcgtaaatcc tgtttttgct ctgaatgccg ggtgataggg atgtctcaaa ctgccccatt
     2941 gttccaaaaa taacagcgtt tgatacggtg atagttcatt atttatccat gtccacagac
     3001 tcttgtagtg catggcttca ttttttaatt gggtgttcca gttttgcctg taggcgtaag
     3061 ccaaagcctc attagcaatc gattcatcta atgcttccct gatttttcgc cattgaaaaa
     3121 acttgctttc cggcattgaa tttttcagtt ttatctgcaa ttggtcaatg aaatcatgga
     3181 catggtgact tgcaatgact gattgaatta atccttcctt aatggaggca tgctgtaaac
     3241 ggttcagggt atttttatga gctaaggtaa taaaataatc cacactattt tgtggtagtc
     3301 ctatgccaat ttcaaatagt aaaaagcgta attgatggct gagttcatga aaattaccgt
     3361 acgccaa
//
LOCUS       DATNSN010000233         3365 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.234, whole genome shotgun sequence.
ACCESSION   DATNSN010000233 DATNSN010000000
VERSION     DATNSN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.234"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(173..640)
                     /gene="sixA"
                     /locus_tag="VAH76_002961"
     CDS             complement(173..640)
                     /gene="sixA"
                     /locus_tag="VAH76_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEO1387537.1"
                     /translation="MKIYLVQHGDSLDKDIDPERPLSDQGQKDIQLLSNFLSPLKIEV
                     SCVYHSGKLRAQQTAESLSGHIFSKNGVESRAGLEPLDDVIPLAKEINQLKQNMMFVG
                     HLPFMNKLVSQLVAEEEHRILVSFKPGSLVCLDKADGINWCIAWMICPELLRE"
     gene            715..1260
                     /locus_tag="VAH76_002962"
     CDS             715..1260
                     /locus_tag="VAH76_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO1387538.1"
                     /translation="MKPKISVFIATSLDGYIARNDGSIDWLIEANSLASPGEDCGYRS
                     FIATIDTIIMGRYSYEKVITFDEWPYGSIPVIVMSSKPIQIPEHLQHIITVSNQSPPD
                     LVSELSLQGIQHVYLDGGITIQGFLQNNLVNEMTITVIPVLIDSGRSLFGSLKNDIKL
                     THRLTTSYPGGFVQINYQIGE"
     gene            1359..1775
                     /locus_tag="VAH76_002963"
     CDS             1359..1775
                     /locus_tag="VAH76_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEO1387539.1"
                     /translation="MDIRYLIYAWGSSILFFIYLYLSYLVIIQRRKYHVLYLTNDDKA
                     FIARFRAHANFAEYVPLTLILMGISIILQVPPLLLAILMFALIIARLGHAYGLIKKEA
                     KKQFKFRIYSVATTFIIMGILAIFNVSKATILYIVI"
     gene            complement(1934..2557)
                     /locus_tag="VAH76_002964"
     CDS             complement(1934..2557)
                     /locus_tag="VAH76_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387540.1"
                     /translation="MAYTKFFAINPYDKKSMSIYQSAKKEFEKDLNHIESSYSELLKD
                     KDFNSALQVEIKELELRLKYVLFNWFFLFKKDKQDLSKQICQLKVDVLRLKTKFMISH
                     LLLNHDKYNLEHYKFVVKHLHNHLEDLNDKIGLIKGIDVDIYNDLFSEMYEWFEQVYS
                     LLNNRYDEFKKEFSIGDKWDDWSYDNSSSEDDWDDNEPLVFLNNTIP"
     gene            complement(2727..3266)
                     /locus_tag="VAH76_002965"
     CDS             complement(2727..3266)
                     /locus_tag="VAH76_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387541.1"
                     /translation="MGRIFLVSLFILLNYNLFASSGSNFILCKSTYALCTTALCKAIP
                     EKKGMASCKCDVKVNQYSVGTKPCTGVTKTKNGFVLSSRYSPISSYVSCQNSRPWAFC
                     LDSPCLVDSQNPKIAFCLCTLVKNKGNYVIVTDHYNKNTCITGIYSSATIKDVQQVTQ
                     FLKRHSELPPYPIKILNAR"
BASE COUNT         1095 a          625 c          528 g         1117 t
ORIGIN      
        1 tagcttaagg aaatattaaa aaacaacaca aaatgaatat aaattaatca aatgatttgc
       61 aattgctggt aaaaaataga agtgacttat cttatatcaa attataatta tctctgatct
      121 aaatcatgga taaaacatag gcagcaagct atgaagatga gaacagtatt cttcattctc
      181 ttaataactc aggacaaatc atccaggcaa tacaccaatt aatcccgtct gctttatcga
      241 ggcaaacaag actgccaggt ttaaatgaaa ccaaaatgcg atgttcctct tccgcaacaa
      301 gttggctgac cagtttattc ataaatggca aatgccctac aaacatcatg ttttgtttta
      361 attgattaat ctcttttgcc aatggtatga catcatcaag cggctcaagc cctgccctgg
      421 actcaacgcc atttttgcta aaaatatgac cagataaact ctctgctgtt tgttgtgccc
      481 ttaattttcc actgtggtat acacaggata cttcgatttt aagcggggaa aggaagttgc
      541 ttaacaattg aatatctttc tgaccctgat cactcaaagg tctttcagga tcaatatctt
      601 tatccaaact gtcaccatgt tgaacaagat aaattttcat ctcacccttt taatttatta
      661 gctaatttat taaagtgtag actcatttga aataacagat aagaaaatct tgtaatgaaa
      721 cctaaaattt cagtgtttat tgcaacaagt cttgacggtt atattgcaag gaatgatggc
      781 tcaatcgatt ggcttataga agcaaattct ctcgcctctc caggagagga ttgtggatat
      841 cgatcattta ttgccaccat agatacgata attatgggtc gatattccta tgaaaaagtt
      901 attacatttg atgaatggcc atatggttcc ataccggtaa tagtaatgag ctcaaaaccc
      961 atacagatac cagaacactt gcagcatatc attacagttt ctaaccaaag tccccctgat
     1021 ttagttagcg aattatccct ccaaggcatt cagcatgtat acctggatgg aggcattact
     1081 atccagggat ttcttcaaaa taatcttgtt aatgagatga ccattacagt aatacctgtt
     1141 ttgattgatt caggccgctc tttatttggc tctcttaaga atgatatcaa actcacacac
     1201 cggttaacta ctagctatcc gggtggtttt gtccaaatta attaccagat tggtgagtag
     1261 ccggatactt cagtcctgtt tactctgtaa ttgtaatgcc gcatgcaatt gctatgttat
     1321 gatggtatag tgccaattta ccagtaacag gataagaaat ggacattcgc tatttgatct
     1381 atgcttgggg cagcagcatt ttattcttta tttatcttta tttaagttac ctggtgatta
     1441 tccaacgtcg taaatatcat gtgctctatt taaccaatga tgataaagca tttattgccc
     1501 gctttcgcgc gcatgccaac tttgctgaat acgttccgct caccttaatc cttatgggga
     1561 tatctattat actccaagtc cccccattgc tattggctat tttaatgttt gcgctgataa
     1621 tagcacgctt gggacatgct tatggtttga ttaaaaaaga agcgaaaaaa caatttaaat
     1681 ttcgcattta ttcggttgca acaacattca ttatcatggg aattttagcg atttttaatg
     1741 taagtaaagc gacgatacta tatatcgtta tataataatt tagatgattt taggtttttg
     1801 gtgatttgcc cccctataat ctgcggatga aacaatgtag atactcatca aatacatatt
     1861 gacttacgct tgctacagaa gccttaccct actgaataat tctcattgaa ccgcatcacg
     1921 atatattaat tcattaagga atcgtattgt ttaagaaaac caggggctca ttatcgtccc
     1981 aatcatcctc gctactacta ttgtcatagc tccaatcatc ccatttatcg cctatagaaa
     2041 actccttttt aaactcatca tatcgattgt ttaatagaga atatacttgc tcaaaccatt
     2101 catacatctc actgaacaga tcgttgtaaa tatcaacgtc aataccttta atcaggccta
     2161 tcttgtcatt gagatcttcc aggtgattat gtaaatgctt aaccacaaat ttgtagtgct
     2221 caaggttgta tttatcatga ttcaacagaa gatggcttat cataaatttt gtttttaatc
     2281 tcaatacatc tacttttagt tgacaaattt gcttgctcaa atcttgctta tcctttttga
     2341 ataagaagaa ccaattaaaa aggacatact ttaacctgag ctccagttct tttatttcta
     2401 cttgcaacgc cgaattgaaa tccttatctt tgagtagttc actataagag gactcgatat
     2461 ggtttaaatc cttctcgaat tctttcttag ccgattggta aatactcata cttttcttat
     2521 catacggatt gatagcaaaa aattttgtgt aagccactaa tattccaggc gttaaagaat
     2581 aaaaaaatgc cctgattata aagtataaac accaaattgg cattaaattt ttcaatttga
     2641 ttctttaaaa aaattatttt aattagcaag ttggtttatg taattcaagc gtaataaaaa
     2701 ttatttaact ctctacaccc tgtgcattac cgagcattca gaattttaat tgggtaagga
     2761 ggtaactcag aatgcctttt caaaaattgt gtaacttgtt gaacatcttt tatagtagct
     2821 gaagaataaa tacctgttat acaggtattt ttattataat gatcggtgac aataacgtaa
     2881 ttccctttat ttttcaccag agtacacaag caaaaggcaa ttttaggatt ttgagaatca
     2941 acaagacaag ggctatccaa acaaaatgcc catggtcttg aattttgaca actgacatag
     3001 gatgagatag gtgagtaacg cgaactcaaa acaaatccat tttttgtttt tgtaactcca
     3061 gtacaaggtt tggtacctac tgaatattga ttaaccttaa catcacattt gcaagaggcc
     3121 atcccctttt tttcaggaat agctttacaa agagcagtgg tacacaaagc ataagtgctt
     3181 ttacacaaaa taaaattgct gccagatgat gcaaataaat tgtaattgag caatataaac
     3241 aaggatacaa gaaatattct tcccatttca atatcccaat taaaaggtaa cttctctgac
     3301 cgttggcaat ttatttatcc atttaaaata ctaataatca gtatagttta tttcccgatt
     3361 acaaa
//
LOCUS       DATNSN010000234         3333 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.235, whole genome shotgun sequence.
ACCESSION   DATNSN010000234 DATNSN010000000
VERSION     DATNSN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.235"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            4..1371
                     /locus_tag="VAH76_002966"
     CDS             4..1371
                     /locus_tag="VAH76_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1387542.1"
                     /translation="MKRTLLNYMDNWLVSTDRKPLVIRGARQVGKTWLVRHLAEKTGM
                     QLIELNFEKQPSYASLFASNDVNQILLNLSTVLNQKIDPPKCLLFLDEIQAAPQLLSK
                     LRWFAEDLPQLAVISAGSLLEFILAEHSFSMPVGRVSYMHLEPLSFDEFLLANDKESL
                     YDYLNQYDLSVELPSAIHEQLTTLFKEYLIIGGMPAVVSNWAAERSLNRVSQIQNDLL
                     ATYRDDFSKYKGRLAIERLDEVLNSIPKMLGQKFVFSRVNKTIQASTIKHVLDLLEKA
                     RISHRVQSSSANGVPLASEIKEKFFKEIFLDIGLCSTALGLSLNQINSTNEILLINNG
                     GIAEQVVGQLLRTIEPPYIEPVLYYWHREEAGSSAEIDYVIQHGNKIIPIEVKAGATG
                     SLKSLHVFMGLKKLPLAMRINSDYPSKTNVDVKDNLGKSVNYTFISIPFYLVGQIHRL
                     LSLLN"
     gene            complement(1561..2766)
                     /locus_tag="VAH76_002967"
     CDS             complement(1561..2766)
                     /locus_tag="VAH76_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO1387543.1"
                     /translation="MGGKRQQGNELQPPRGVSIRQWETGSAIRIIFSYRGVQCRETLK
                     LEPTKANLKYAERLRGEIINAIALGTFNYADYFPDSKRAKLFGHAIIKATTAELLKDY
                     MAIAERTLEASTYNGYRKVCEAHLYPAFGKIPIRELSPVIIRNWITGLNVTVKTVRNI
                     LTPLRNMLDQAMNDGIIERNPLDRLVLSKLLNKKTSKSDWEVDPFDQDEIKAILDAAT
                     GQARNLFQFAFFSGLRTSELIALEWNDIDWLKGTVRVSKAVVLKQEKGTKTKSGVRDV
                     LLLPPAVDALKNQKAFTYLEGSRVFYNPRTNSPWETDGQIRKTCWTHLLKKAGVRYRN
                     PYQTRHTYASMLLSAGENPLWLATQMGHRDTEMIIKHYGRWIPDKSTVAGYQPVSDWN
                     LSMGTTKIG"
     gene            complement(2715..2921)
                     /locus_tag="VAH76_002968"
     CDS             complement(2715..2921)
                     /locus_tag="VAH76_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO1387544.1"
                     /translation="MKWVKLKKYCQDSGDTSDAVHAKRKRGIWLDGVQCKLGPDGNIW
                     INLVEVEKWVENGNKATNCSLRAV"
     gene            complement(2928..3209)
                     /locus_tag="VAH76_002969"
     CDS             complement(2928..3209)
                     /locus_tag="VAH76_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387545.1"
                     /translation="MDNNSKAQIYKGIIQYLLESTNYTLKNIADLSNSPIKNIRAIYC
                     DNFVPLNFSSELQLVRLYQMILEIHTQEKQFKKYLPLPKGFRQLSASME"
BASE COUNT         1024 a          657 c          605 g         1047 t
ORIGIN      
        1 taaatgaaac gaactctatt aaattatatg gataactggc tggtttcaac tgaccggaaa
       61 ccattagtca ttcgtggggc aagacaagtt ggtaaaacat ggctagtaag gcacttagct
      121 gaaaagacag gaatgcaatt aattgaactc aacttcgaga agcaaccttc ctatgccagt
      181 ctttttgctt ctaatgatgt aaatcaaatt ttattaaatt taagtacggt attaaatcag
      241 aaaatagatc ccccaaagtg cttattattc ctagatgaaa tacaggcagc acctcaactg
      301 cttagcaaac tccgttggtt tgcagaagat ttaccccaac tagcagtaat ctccgctggc
      361 tctctattag aatttattct agcggagcac tcatttagta tgccagttgg gcgagttagc
      421 tatatgcatt tggaaccatt gtcatttgat gaatttttat tagcaaatga caaagaatca
      481 ctttatgact atttaaatca atatgaccta agtgtcgagc tcccctctgc gattcacgag
      541 caattaacta ctttatttaa agaatatctg attatcggcg gtatgcccgc tgtagtctct
      601 aattgggctg ctgaacgctc tctgaataga gttagtcaaa tacaaaatga cttattggcc
      661 acctatcgcg atgacttttc aaaatataaa ggtcgtttag cgatagaaag attagacgaa
      721 gtacttaatt ctattccaaa aatgcttgga caaaaatttg tatttagtag agttaataaa
      781 acgattcaag catcgacaat aaagcatgta ttagatctgt tagaaaaagc ccgaattagc
      841 caccgagtac aaagtagctc agcaaatggt gttcccctcg catctgaaat taaagaaaaa
      901 ttttttaaag aaattttcct tgatataggc ttatgtagta cagcactagg actgagttta
      961 aaccaaatta attcaacaaa tgaaattctt ttgattaata atggcggcat cgctgaacag
     1021 gtagttggcc aattgctaag aacaattgaa ccaccctata ttgaaccagt tttgtattat
     1081 tggcatagag aagaggctgg ttcaagtgct gaaattgatt atgtaatcca acatggtaat
     1141 aaaattatac ctattgaagt aaaagctggt gccacaggaa gcctaaagtc actacatgtt
     1201 ttcatgggat taaaaaaatt acctctcgcc atgcgaatta attctgatta tccaagtaaa
     1261 actaatgtag atgtaaaaga taatttaggg aaatccgtaa actatacttt tatatccatt
     1321 cccttttatc ttgtggggca gatacataga ttacttagtt tgctcaacta acatatatag
     1381 tgcaaaaaac caagtgcaac tcatatgagt ttgacattat ttgtcagtat cactaattaa
     1441 attagtaatg ccgactgatt ttgtcaaaaa tcgagcgttc gcaagaaaca tgtactctgc
     1501 aatttgattg agtaacttta catatcttgt taagaatctc aactagaccg cataaattaa
     1561 ctatccgatc ttggtagtac ccatgcttaa attccaatca ctcaccggtt gatatccagc
     1621 aacagtggac ttatcaggga tccaccgccc ataatgtttg ataatcatct ccgtatctct
     1681 atgccccatc tgtgtggcta gccataaagg gttctcaccg gcagaaagca gcattgatgc
     1741 gtaagtatgc cgcgtttggt aaggattgcg atagcgcacg cctgcttttt tcaaaagatg
     1801 cgtccagcaa gttttgcgaa tctggccgtc tgtctcccac ggagaattgg ttcttgggtt
     1861 ataaaaaacc ctagagcctt ccagataggt aaatgctttt tgatttttta atgcatcaac
     1921 agctgggggt agtaataaca catccctcac ccctgatttt gttttagttc ccttttcctg
     1981 ttttaaaact accgccttag atacacggac agttcctttg agccaatcaa tatcattcca
     2041 ctccaatgca ataagctcag atgttctcaa acctgaaaaa aaagcaaatt gaaacaagtt
     2101 tcttgcctgt ccagttgctg catccagaat tgccttaatt tcatcctgat caaatgggtc
     2161 aacctcccag tcacttttac tggttttctt attgagcaat ttagaaagca ccaatctatc
     2221 aagggggttg cgttcgataa ttccatcatt catagcttgg tccagcatat ttcgcagggg
     2281 agtcaaaata ttgcgaaccg tttttaccgt aacatttaac cctgtaatcc agttacggat
     2341 aatcacaggt gataattcac gaatgggtat tttaccaaaa gcaggatata aatgagcctc
     2401 acaaactttt ctatagccat tgtaagtact ggcctccaaa gttctctcag caatggccat
     2461 gtaatccttg agtaactcag ccgttgtggc tttgatgatt gcatggccaa ataactttgc
     2521 acgtttggag tcagggaaat aatcggcata gttaaatgtt cccagggcga ttgcattaat
     2581 gatctcgccc cgcaaccgct cggcatattt tagattcgcc ttggtgggct ccagtttcaa
     2641 cgtctcgcgg cactgtactc cgcgataact gaagataatc cgaattgcgg atccagtttc
     2701 ccactgacgg atacttacac cgcgcggagg ctgcagttcg ttgccttgtt gccgttttcc
     2761 acccattttt caacctcaac taaattaatc catatgttgc cgtctggtcc taatttacat
     2821 tgtacaccat cgagccagat accacgtttg cgtttagcgt gtactgcatc agaagtatct
     2881 cctgaatcct gacaatattt tttaagtttt acccatttca cgatatgtca ctccattgat
     2941 gcactgagtt gccgaaaacc tttaggaaga ggtaaatatt ttttaaactg tttttcttga
     3001 gtgtgtattt caagaatcat ttgatataac cgtactaatt gtagttcgga agaaaaatta
     3061 agtggtacga agttatcgca ataaattgca cgaatatttt taattggtga attagaaagg
     3121 tctgcaatat tttttaaggt gtagttagtt gactccaaaa gatattgaat aattccttta
     3181 taaatttgtg ccttggaatt gttatccatg aataatactc ctttgactat cttatccagt
     3241 agctaactta ttcattaaat gtcaaattat ctcgcaattt tatttggtga actaatcagc
     3301 aacatgatat attggcgata attattctat tta
//
LOCUS       DATNSN010000235         3271 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.236, whole genome shotgun sequence.
ACCESSION   DATNSN010000235 DATNSN010000000
VERSION     DATNSN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.236"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..347
                     /locus_tag="VAH76_002970"
     CDS             <1..347
                     /locus_tag="VAH76_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387546.1"
                     /translation="GKIVIDNKTNYPVKDNSRKIAVQWVATVEATQKANKIIINGSKL
                     DLSSLKIITQKGKTQLTLPNHSHYFRVVVWSTGKQQPDLLTNWVDIVPNKTYIINQDQ
                     LVPAILMSGTGC"
     gene            complement(619..1530)
                     /locus_tag="VAH76_002971"
     CDS             complement(619..1530)
                     /locus_tag="VAH76_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEO1387547.1"
                     /translation="MTIQKGLHERRALKISIAVTFLLAVVGILFGLLSGSLAIVFDGM
                     FNMVDTVISILAFFVARLLTSKGNRRFQYGYWHIEPMVLVLNGSILILLCTYALVNAI
                     GSLMSGGHELNFDWAFVFALLVFFLSTGMYFYLVKTNRKIKSEFLRLDIQSWLMSALI
                     STSLLLAFGIAALLHGGAYGYFTPYIDPLILAILTAFLIFVPMAAVRDAMRDIFRMAP
                     VDLDERIREFLDELIKQHDFKTYTSYVAKIGRAQFIEIHIVVPMDYPISSIETLDKIR
                     NEIALAIGEDTPQRWLTIAFTANENWI"
     gene            complement(1687..3093)
                     /gene="dbpA"
                     /locus_tag="VAH76_002972"
     CDS             complement(1687..3093)
                     /gene="dbpA"
                     /locus_tag="VAH76_002972"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEO1387548.1"
                     /translation="MIQSEHNDQHLSFSQFPLRQELIKSLASSNYENMTPIQMQSLPI
                     ILRNEDIIAQAKTGSGKTAAFALCLLNNLKISFFAVQALVLCPTRELAEQVSQAIRRL
                     ACLMPNVKIINLSGGIPMQPQLGSLRHGAHIIVGTPGRILKHLKNASLDLSQVKALVL
                     DEADRMLDMGFFDDIKNIISICPKQRQTLLFSATYPEEIKRLSKQFMKDPKEVHVVTP
                     PEEIDIEQHFYEVSRQAQKFPLLKSLLLHYRPVSVLIFCNTKQQTVEVTDQLINEGFS
                     AIALNGDMEQIERDLAVLRFANQSCSILVATDVAARGLDIKELSAVINFDLAFDHDIH
                     IHRIGRTGRAGSKGIALSIVTPADAQRICAIEDNYPHPIHWGNINELENHNTACLVPE
                     MVTLCIASGKKDKIRPGDILGALTKDAGLAGNTIGKINITAMYSYVAIHHSQADKAYQ
                     YLQSGKLKGRKVNVRKIN"
BASE COUNT         1046 a          606 c          609 g         1010 t
ORIGIN      
        1 ccggcaaaat tgtaattgat aataaaacga attaccctgt aaaagataac tccagaaaaa
       61 ttgccgtaca atgggtagca accgtagagg ctactcaaaa agccaataaa attataatta
      121 atggttcgaa attggaccta agctccttaa agataatcac tcaaaaaggg aaaactcagc
      181 tcactctgcc taatcatagt cattatttta gagtggtggt ttggtcaact ggcaaacagc
      241 aaccggattt acttacaaat tgggtagata ttgttcctaa taaaacttat ataataaacc
      301 aagaccaact tgtgcctgcg attctaatgt ctgggacagg ctgctaaaac cacctgtctc
      361 tatgcagtga aaagctatca atacctcatg tacctaagcc cgttagtgat aatgggctct
      421 aacccaaaat agcgatataa cgtggtcaaa gacccgctaa gtgcttccat cacaagatga
      481 agttttgttg ttggcaaaca agttgttcta tttaatctga ttacatttac taaaattaag
      541 agttcatttt gctgtactct caattttctg tagcatgttt ttagaaatat cctgatggaa
      601 acttgaataa aacgcttgtt aaatccagtt ttcatttgca gtaaaggcaa tggttagcca
      661 acgttgtggc gtatcttctc caatagccaa cgcgatttca ttacgaattt tatccagggt
      721 ttcaatgctc gaaattgggt aatccatagg tactactata tgtatttcaa taaattgtgc
      781 gcgtcctatt tttgcaacgt agcttgtata agtcttgaaa tcatgctgtt taataagctc
      841 atctaaaaat tctcttattc tttcatcaag atccacagga gccatgcgaa atatgtctcg
      901 catggcatca cggactgcgg ccataggcac aaaaatcaga aatgcagtaa gaattgctaa
      961 aataaggggg tcaatgtaag gggtaaaata tccataggcc ccaccatgca acaaggctgc
     1021 gatgccaaat gccaatagga gagaagtgga aatcaacgca gacattaacc aactttgaat
     1081 gtctagtcgc aagaattcgg acttaatttt gcggtttgtt ttaactaaat aaaaatacat
     1141 acctgttgat aaaaaaaaca ccaaaagtgc aaacacaaat gcccaatcaa aattgagttc
     1201 atgtccacca gacatcaaac tgccaatcgc gtttaccagt gcataagtac aaagaagtat
     1261 taggatactg ccattcaaaa caagaaccat aggttcaata tgccagtaac catattgaaa
     1321 tctccgattc cccttacttg ttaaaagacg ggcaacaaaa aaagcaagga ttgatataac
     1381 ggtatccacc atattaaaca taccgtcaaa gacgatagcc aatgaaccgg acaatagccc
     1441 aaataaaata cctaccacgg ctaagagaaa ggtaacagcg attgatattt taagagctct
     1501 tcgttcatga agtccctttt gtattgtcat ttgagtgata tcctgatttt aacaatgctt
     1561 tatctactta tactattgtt tcaacagcca ctgataaatc cataggcttg tattattagg
     1621 ccttttacat cataactgct tctttattta attgttgcag taagtttttc gcatggttta
     1681 gaaaaatcaa tttattttac gtacattaac tttgcgtcct tttaattttc cactttgtaa
     1741 atattggtat gctttatcag cttggctgtg gtgaattgca acataggagt acatagcagt
     1801 gatgttaatt ttgccaattg tgttgcctgc taatcctgca tctttggtta atgccccaag
     1861 gatgtcacca gggcgaattt tatctttttt accggaagcg atacagagtg taaccatttc
     1921 tggcactaag caagcagtgt tgtgattttc taattcatta atgtttcccc aatgaatggg
     1981 gtgcgggtag ttatcttcaa tagcgcaaat tcgttgtgca tctgcaggag ttacaatgct
     2041 taaggcaatg cctttactcc cagctcgtcc tgttcgacca atacgatgaa tatggatgtc
     2101 atgatcaaaa gcgagatcaa aattaatcac tgcggaaagt tcttttatat caagtcctcg
     2161 tgctgcaaca tcagtggcga caagaataga acaactttgg ttggcaaaac gcaagacagc
     2221 aagatcacgt tcgatttgtt ccatatcacc atttaaagcg atggcactga agccttcatt
     2281 gatgagctgg tctgtgactt ctactgtttg ttgcttggta ttacagaaga ttaatactga
     2341 aacgggccga tagtgtaaca gtaatgattt taataaggga aatttctgag cctgtctcga
     2401 gacttcataa aaatgttgtt caatatcaat ttcttcagga ggagttacca catgaacttc
     2461 tttcgggtcc ttcataaatt gttttgaaag ccgtttgatt tcttcaggat aagtcgcaga
     2521 aaaaagcagg gtttgtcgtt gtttggggca aatcgaaata atgtttttaa tgtcatcaaa
     2581 aaaacccata tccagcattc tgtctgcctc atccaaaact aaagctttta cctgagataa
     2641 gtctaaagag gcgtttttta aatgcttaag tattctcccc ggtgttccta caataatatg
     2701 agctccatgc cttaatgaac caagctgagg ctgcattggt ataccaccag ataaattaat
     2761 aattttgaca ttaggcatca aacaggctaa tcgacggata gcttggctta cttgttccgc
     2821 aagttcacgg gtagggcaaa gaaccaaggc ttgtactgca aaaaatgaaa tttttaaatt
     2881 atttaataaa cacaaagcaa aagcagcggt ctttccactc cctgtttttg cttgagctat
     2941 tatatcttca tttctaagaa ttatgggcag gctttgcatt tggatagggg tcatattttc
     3001 ataatttgaa gaagcaagac tttttattaa ttcttgacga agggggaatt gagagaatga
     3061 taggtgttgg tcgttgtgtt ctgattgaat catggaaagt gcttattgtt atattataat
     3121 aggtgaatag cttagtactt cttagtgcaa ttttaaatca atttattttg gacaagctta
     3181 atctgatgct tacaattgtc ttttctactt gaatccgtgt agatcaaaat aacattaaaa
     3241 tcatctcaaa gacatcaaca gagagtgatt t
//
LOCUS       DATNSN010000236         3260 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.237, whole genome shotgun sequence.
ACCESSION   DATNSN010000236 DATNSN010000000
VERSION     DATNSN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.237"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..922
                     /gene="rpsB"
                     /locus_tag="VAH76_002973"
     CDS             158..922
                     /gene="rpsB"
                     /locus_tag="VAH76_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO1387549.1"
                     /translation="MNNVSMRELLEAGAHFGHRTRFWNPKMSEYIFGSRNKIHIINLE
                     KTLPMLNDVTNYVSRLAANKAKILFVGTKRAAQDSIREHAKRCGMPYVDHRWLGGMLT
                     NYKTVRQSIFRLKELKEMKEKGLFNDMIKKEALMLTRELEKLERSLGGIENMGGLPDA
                     LFVVDVGFEHIAVEEARRLRIPVIGVVDTNNSPDNIDYVIPGNDDSMRAVDIYVRCVA
                     DAILDGKNSNTVGRVSSDSEFVEVTSNSNEEEKSGE"
     gene            988..1866
                     /locus_tag="VAH76_002974"
     CDS             988..1866
                     /locus_tag="VAH76_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO1387550.1"
                     /translation="MSTISAALVMQLRERTGAGMMECKKFLIATNGDIEQAIIEMRKA
                     GQAKADKKADRVAAEGIIVIARSSDERTAVMLEINSETDFVARDENFTNFANAVADVA
                     LTSLPKNIEDLSNQALSSGATVEQARQELVAKIGENIKLRRLEKMHCDGVIGYYLHGS
                     RIGVMVALKNGSEALAKDIAMHVAASKPMVVSRDQVPAEAIENEREIFTAQAKESGKP
                     QEIIDKMIDGRINKFIDEVSLLGQPYVKDPNIKVGQLLKEKNAEVISFVRYEVGEGIE
                     KKEDNFVEEVMAQVRT"
     gene            1863..2606
                     /gene="pyrH"
                     /locus_tag="VAH76_002975"
     CDS             1863..2606
                     /gene="pyrH"
                     /locus_tag="VAH76_002975"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO1387551.1"
                     /translation="MMNESQPKLKYKRILLKFSGEALMGKSQFGIDPSVLDSLARDIA
                     ELIHMGVEVGLVLGGGNLFRGKALSQAGVGRVTGDHMGMLATVMNALALRDALERIDL
                     PARIMSAIPMLGVVDPYHRRKAITHLRNGQVVIFAAGTGNPFFTTDTAACLRAIEIGA
                     DIVLKATKVDGVYSADPLKNSDAKRYDYLTYKEVLTKGLEVMDSTAICLCQDQGMPLQ
                     VFDMAAPKALKRIVTGERVGTIVGANHDQ"
     gene            2596..3153
                     /gene="frr"
                     /locus_tag="VAH76_002976"
     CDS             2596..3153
                     /gene="frr"
                     /locus_tag="VAH76_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO1387552.1"
                     /translation="MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDY
                     YGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPM
                     PPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
                     QKLTDRYISEVDAVLAEKEKDLMEI"
BASE COUNT         1057 a          527 c          774 g          902 t
ORIGIN      
        1 gtcatgtaca gagagcgatg ttaatttcat tgctgaatta gcacgaatat gttataaata
       61 ccgcgcttta aatacacaca cgtttcgtca catgcgatag ggtcccttgg tgggtttcgt
      121 atgggaagcg tggaggctta accctggaga tatatatatg aacaatgtta gtatgcgtga
      181 attgctcgaa gcgggtgctc attttgggca cagaacacgt ttctggaatc caaaaatgag
      241 tgaatacatt tttggctcaa gaaataaaat ccatatcatt aaccttgaaa aaacattacc
      301 catgctgaat gacgtaacga attacgttag tcgattggca gcaaacaagg caaaaattct
      361 ttttgtaggc actaaaagag cagctcaaga cagcattcga gagcatgcaa aacgatgcgg
      421 aatgccttat gtagaccata gatggttagg aggcatgctg accaactata aaacagtccg
      481 tcaatcaata tttcgtttaa aagaattaaa agaaatgaag gaaaagggat tgttcaatga
      541 catgattaag aaagaagcgc taatgctgac aagagagctt gaaaagctgg agcgtagtct
      601 tggtggaatc gagaacatgg gcggcctacc tgatgcgtta tttgtggttg atgttggttt
      661 tgaacatatt gctgttgaag aagcgcgtcg attaagaatt ccggtaattg gtgttgttga
      721 taccaataat agccctgaca acattgatta tgttatacca ggcaatgatg attctatgcg
      781 tgctgtggac atttatgttc gctgtgtcgc tgatgctatt ttagatggta aaaatagcaa
      841 tactgtagga agagtatcat ctgattcaga gtttgttgag gtcacttcaa attctaatga
      901 agaagaaaaa tccggagaat aattactatt tgaataaagg gggcgttaat ttaataagcc
      961 ccctttttgc tatgtggaga aatcaaaatg tcaacaatta gtgctgcatt agtaatgcaa
     1021 ttgcgtgagc gcacaggcgc aggtatgatg gaatgtaaaa aatttctaat agctactaac
     1081 ggtgatatcg agcaagcaat cattgaaatg cgtaaagctg gccaggcaaa agcagacaaa
     1141 aaggcagata gagttgcagc agaaggtata attgttattg cacgttcttc tgatgaacgt
     1201 acagcggtaa tgcttgagat taatagtgaa acagactttg ttgcgcgaga tgagaatttc
     1261 actaattttg caaatgctgt tgccgatgtt gcattaacca gccttccaaa aaacatagaa
     1321 gacttgtcca atcaggcatt atcttctggt gctactgttg agcaagcaag acaggagctg
     1381 gttgctaaaa ttggtgaaaa tattaaatta cgccgattgg aaaaaatgca ctgcgacggt
     1441 gtaatcgggt attatttgca tggttcgcgt ataggagtta tggtcgcatt aaaaaatgga
     1501 agtgaagcat tggctaaaga tattgccatg catgttgctg ccagcaaacc gatggtagtt
     1561 agtcgtgatc aggtaccagc cgaagcgatt gaaaacgaaa gagaaatatt cactgctcaa
     1621 gcaaaagaga gtggtaagcc gcaagagatc attgacaaga tgattgatgg acgaatcaat
     1681 aaatttattg atgaggtaag cttgttagga caaccttatg taaaagatcc taacattaaa
     1741 gtgggtcaac ttctaaaaga gaaaaatgct gaagtaatat cctttgttcg ttatgaagta
     1801 ggggaaggaa tagagaaaaa agaggacaat tttgttgaag aagtgatggc tcaggttcgt
     1861 acatgatgaa tgaaagtcaa cctaaattaa aatacaaacg cattctgctt aaatttagtg
     1921 gtgaggcgct gatggggaaa tctcaatttg ggattgatcc ttcagttctt gactcattag
     1981 ctcgagacat agcagaatta attcatatgg gtgtggaagt tggtttggtt ttaggtggag
     2041 gcaatctgtt cagagggaaa gcgctctctc aggcgggcgt aggacgagtg acaggagacc
     2101 atatggggat gctggccact gttatgaatg ctttggcact ccgtgatgct ttggagcgaa
     2161 ttgatctgcc cgctcgtatt atgtcagcta ttccaatgct tggggtagtt gacccatacc
     2221 atagacgaaa agctatcact cacttgcgca atggtcaggt ggtgattttt gcagctggaa
     2281 caggaaatcc cttttttaca acagatactg ctgcttgttt gagggcaatt gagataggcg
     2341 cagatattgt tttaaaagca actaaggtgg atggagttta ttctgctgac ccacttaaaa
     2401 actctgatgc aaagcgttat gattatttga cttacaaaga agttttaacc aaaggtttgg
     2461 aagtgatgga ttccaccgca atttgtttgt gccaagatca gggaatgcct ttacaggtgt
     2521 ttgatatggc ggctcctaaa gcattaaaac gaattgtaac tggagaacga gtaggaacta
     2581 tagtcggagc taatcatgat caatgagatt aagcaagatt cagaaaaacg catgaaaaag
     2641 actattgagg ctttgcatac ggatatgtca aaaatccgta ccggcagggc gaatgccagt
     2701 cttttagatc atgtcatggt tgattattat ggaagtccta cacctttaag ccaggttgcc
     2761 aacatcacta ccagtgactc ccgaactatt ttagttacac cctgggaaaa atctatggtc
     2821 gcagctattg aaaaggctat actaaactct gatttgggtt tgaatcctgc aactgctgga
     2881 acagccattc gagttccgat gccaccttta acagaggaac gcagaaagga attgattaaa
     2941 gttgttcgcc atgaagggga acaaggaaga gtttcaattc gaaatatcag aagggatgcc
     3001 aataatcagc taaaggaact tgttaaagaa aaagcaattt ctgaagacga tgaacgacgt
     3061 gcagcagaag cgatccaaaa gctgactgat agatatattt ctgaagttga tgcagtatta
     3121 gccgagaaag aaaaagattt aatggaaatt taaggtatag atgttactcc ttttatataa
     3181 tagagctata gttcttgttt ttagtacaag aaaaaacata ctctaattat ataatgctaa
     3241 atttttgtca ttcccgaccg
//
LOCUS       DATNSN010000237         3236 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.238, whole genome shotgun sequence.
ACCESSION   DATNSN010000237 DATNSN010000000
VERSION     DATNSN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.238"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..562)
                     /locus_tag="VAH76_002977"
     CDS             complement(<1..562)
                     /locus_tag="VAH76_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387553.1"
                     /translation="MGSSYNDWLKSSYSELKKINIIENLIKENNFARAKMLLNNLDLT
                     TLIKYTELSKTITDFCEEAEQADIWRTHLQNFNEEHFSFEEYPPLTVSQLVKGIYFYG
                     QAAECREEEGKPFGDNELEFLKKSAYQHCFYAYNSLSTWAYEKYKMGLNDYSLLTLHY
                     AQKACQYHWTPGYLLFYKTCLNLAILS"
     gene            865..3090
                     /locus_tag="VAH76_002978"
     CDS             865..3090
                     /locus_tag="VAH76_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO1387554.1"
                     /translation="MAESKLEIVNKTEREESVILDGNGILRCYNMFPTQFGSIEEMID
                     YLPQLQAMGFNAVWINPVQTAGDVQGLFKRDKTIGVRAYNEVTRSLYAMTDCDLISPY
                     FNPIPPNVDPKLKKQLDMEAMQKFTSEARNNGLVPMFDLVLNHVAADSRHRKEHPEWF
                     QKDVHPDFKDAIAFDYSDPQIRDEIIEQFWKPYIYKYMIDYGFEGVRIDAVGYLNPEL
                     RKQIYAYIYDLAEQFNKPKPVILDELLFSGSQKLTDVVDGLLLPEKGPTHITRGTYYA
                     QRDGYGGLPGWCKEEEGVKAQVVFLDKQKKLHPHTKGGCIAFSGNHDHNSLAMTILEE
                     MAEHRLANHHTLHGAQEKFKHERSYDYDEALESVLRYSFVKDIQNEILNGNESTIKEV
                     ETRMREKIAFCALTSSGGWYALSGDETGDLLAKPVFRRVHALDQTYYAQRTHIIFDKK
                     YPDNKVAMEVLTEMAIENIQKEKAGSIYNSLQHDKQSQERLLVPYLETLQNQINCGDT
                     VVCKAFQEKLAQKNITIGFTEENYNPAPRTPQNGWNGQHDMKKFMFEINSILSSLPSS
                     NMGFWSEVIPIQSKPGLLAVVRKNGLGLSSETDIAIVNMDPSQKVELTRDDIHQLACN
                     FQKRVIPEYTYYNDGKDKQYNWHTNNPDFNMAYHTVMSCIEAKRIHVDDSIKTNFPVH
                     SANPYGMFSKKQSSKMNIAKIAKMSGEDTTLIEKGTNDTEGIIHDDEQIQPTITVEIK
                     K"
BASE COUNT         1114 a          534 c          618 g          970 t
ORIGIN      
        1 tcgataaaat ggctaaattt aaacaagtct tataaaataa taaatatcct ggagtccaat
       61 gatattgaca ggccttttga gcatagtgca aggttaacaa agaataatca ttcaaaccca
      121 ttttgtattt ttcataagcc caggttgata aagagttata agcataaaaa cagtgttgat
      181 aagcgctctt tttaagaaac tccaattcat tatcaccaaa tggtttacct tcttcttccc
      241 ggcattcagc tgcttgtcca taaaaataga tcccttttac taattgggac actgtgagtg
      301 gcgggtattc ttcaaatgaa aaatgttctt cgttaaaatt ttgcagatga gtccgccaaa
      361 tatcagcttg ctctgcttct tcacaaaaat ctgtaattgt tttacttaat tcggtgtatt
      421 taatgagggt agtgagatca agattattta agagcatttt cgccctggca aaattgtttt
      481 cttttatcag attttcgatg atgtttattt tctttaattc gctatatgac gatttcagcc
      541 aatcattata ggagctaccc ataaatcctc cacaaaattt ttaagttcaa gacatgttag
      601 agattaaagt ctaagtcttg acaccaatat ttgttaataa ttggtgatat aaatacggta
      661 atattttaac acaatttgat ttatttaaaa acttctaata tcaatttagc cgctcaataa
      721 ccttcaaaaa tgagaaaaat tacaaaataa cttcccctat tgattataca acggctatgt
      781 ttgcttgatt tttaagctca agaggcataa tcctgagatg tgtactatat tttatctaag
      841 agataatgat atgggcatat tattatggca gaatcaaaat tagagatagt caataaaaca
      901 gaacgtgaag aaagtgtaat cttggatggt aatgggattt tacgttgtta taacatgttt
      961 cctactcaat ttggaagcat agaggaaatg attgactatc ttcctcaatt acaggctatg
     1021 ggatttaatg ctgtatggat aaatcctgta caaacagcag gggatgttca gggtttattt
     1081 aagcgtgata agacaattgg cgtcagagct tataatgagg tgacaagaag tctatatgct
     1141 atgacagatt gtgatctgat aagtccttat tttaacccga tccctcccaa tgttgatcct
     1201 aagttgaaaa agcagctgga tatggaagca atgcagaagt tcacatcaga agcccgcaat
     1261 aatggtttgg taccgatgtt tgatttggtt ttaaatcacg ttgccgccga ttcacggcac
     1321 agaaaagaac atcctgaatg gtttcaaaag gatgtccacc ctgattttaa ggatgctatc
     1381 gcttttgact acagtgaccc tcaaatccga gatgaaatta ttgaacaatt ttggaagcct
     1441 tatatttata agtacatgat tgattatggc tttgagggtg ttcgcattga tgctgtgggc
     1501 tatttaaatc cagaattacg caaacaaatt tatgcttata tttatgatct tgcggagcag
     1561 tttaataaac ctaaacccgt tatattggat gaacttcttt tcagtgggag tcagaagctt
     1621 accgatgtgg ttgatggttt gttgcttcca gaaaaagggc caacccatat tactcgcggt
     1681 acctattatg cgcaaagaga tggttacggt ggtttacccg gatggtgtaa ggaggaggag
     1741 ggagttaagg ctcaagtagt attcctggat aaacaaaaga aattgcatcc ccataccaaa
     1801 ggcggatgta ttgctttctc cggtaatcat gatcataact ccttagccat gacaatattg
     1861 gaagaaatgg cagaacaccg tttggcaaat catcacactt tgcacggtgc gcaagaaaaa
     1921 tttaaacatg aaaggagcta tgattatgac gaagctttag agtcagtcct gcgctattct
     1981 tttgtgaaag atattcaaaa tgagattcta aacggtaatg aatcaacaat caaagaagta
     2041 gaaacaagaa tgcgtgaaaa aatagctttc tgtgctttaa catcaagcgg cgggtggtat
     2101 gctctatcag gtgatgaaac aggcgattta ttggcaaaac ctgtattcag aagggtccat
     2161 gctctggatc aaacctatta tgcacaacgc actcatataa tctttgataa aaaatatccc
     2221 gacaacaaag tagctatgga ggtattaacc gaaatggcta tagagaatat ccaaaaagaa
     2281 aaagctggaa gcatctataa cagtttacag catgataaac aatcacaaga gagattatta
     2341 gttccttatc tggagacttt gcaaaatcaa attaattgtg gtgatacagt agtttgcaaa
     2401 gcatttcaag aaaaacttgc ccagaaaaat ataacgattg gtttcacaga agaaaattat
     2461 aatcccgctc ccagaacacc tcaaaatggt tggaacggtc aacatgatat gaaaaaattt
     2521 atgtttgaaa tcaatagtat attaagttcc ttgccaagtt ccaatatggg attttggagt
     2581 gaagttatcc caatccagtc aaaaccaggg ttgcttgcag tagtacgtaa gaatgggcta
     2641 ggcttaagtt ctgaaactga tattgcaatc gttaatatgg atccaagtca aaaagttgag
     2701 ttaaccagag acgatattca tcaattagcc tgtaatttcc agaaacgagt aattcctgaa
     2761 tatacttatt acaatgatgg taaagacaaa cagtataatt ggcatacgaa taatcctgat
     2821 tttaatatgg cttatcatac agtgatgtcc tgtatcgaag ccaaaaggat acatgttgat
     2881 gattcgatca aaactaattt ccctgttcat tcagcaaatc cctatggcat gtttagcaaa
     2941 aagcaatcat cgaaaatgaa tatagccaag atagcaaaaa tgtctgggga agataccact
     3001 ttaattgaaa aaggtactaa tgatactgag ggtatcatac acgatgatga gcaaattcaa
     3061 cccacaataa ctgtagaaat caaaaaataa agaagtacta taataaaagt tgtttcaact
     3121 tggcgacagg gtattatttt gataaatcgg atactctgca atataactaa ttacaaagac
     3181 cgtataacaa gtagggaatg ctcggccata ataagcggta agatacagga cttacg
//
LOCUS       DATNSN010000238         3228 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.239, whole genome shotgun sequence.
ACCESSION   DATNSN010000238 DATNSN010000000
VERSION     DATNSN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.239"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            443..1822
                     /locus_tag="VAH76_002979"
     CDS             443..1822
                     /locus_tag="VAH76_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEO1387555.1"
                     /translation="MRIHNLLAISLGIFSPLIHPGGVPLCVLSDHCITVVEPLFDVLP
                     ETSAKSSFLRSGTTSHNDTCSEKEIYQSENVFRRNNKLELYFNPNHSPIYDPYCNQIY
                     TQTSGAVYTQAPITKFSNGVLILHGAVTVKAKIDRIIPGIWPAIWMLPQDTAFTDLDG
                     GASGVWPTSGEIDTLEMAGYSGYYNPPATYLTTLHFGPTPGDDIPRGYTTVYKNDAQQ
                     IVPLTDKEHEFTMEWDITLKNTVNGQVTFLNPANVASIRLSMYLDGRLYQSDKLIWNS
                     YSPPVCDNGLVGTGRSDGTTCGVPVFQIPSSFDPLPLNDALPLEVFTRGFNSGFRLIS
                     NVASGGPIFSGDPIGEAGAREPVSLFISEIYLQIINNLAFVPNIFNGAKVSWLTPTIP
                     GETIDHYTLEISGYKFNINKQFPSTQNQFTIPAHTLYGNRSYHAVVTAVGNLGTTVLV
                     GDATVKIRP"
     gene            1847..2524
                     /locus_tag="VAH76_002980"
     CDS             1847..2524
                     /locus_tag="VAH76_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEO1387556.1"
                     /translation="MSLLKFLVYPLSLGFSVSSFAAQPYLGLQVGSNFFTQKQTITAF
                     DVTGEINRWQSNDGFEGGLLLGVRWVTKPTYLLDAELFASYNADDISVKNTLTGPPFV
                     DHLDQNLGYSFGARILPAYVIRPNLSLFGLLGYIQTQLKSNSLDGVTEGLSGNQSVIF
                     NGVQCGFGINIDFNSGFRLRMQDSYAFYGSRNITTPSIGLDNQLISFNIRPYANQVSL
                     SLIKQFS"
BASE COUNT         1037 a          587 c          585 g         1019 t
ORIGIN      
        1 gctctattta tcgaatagtc gttatttcat gtatgcatac caattcattg tacggtaaaa
       61 aattaattat taacgcattg attttttaat acttattagg tatttttgca acaccaaaat
      121 tacctacagc ctttgtaaat aaggaataaa tcattctaag gttagttgct aattaatact
      181 agagcctaca ttcccctctg tatacgcaaa atcgagtttt aaagtaagcc atcaatagaa
      241 ttaatggcat acgttaggta tatttctgca cgtaaagtgc gaaaaatagg cggctcaaat
      301 tgaagtgtga ataaactcta aaacctcaca aaaaactggt gaaccttcaa cagcaacttg
      361 tttagagtat atggccttta aaaaaaccaa tcgcaactta aaggtaaata tcattaagaa
      421 ttaaatataa aggagtataa taatgaggat ccataattta ctggctattt cactaggcat
      481 attttcaccg ctaatccatc ccggaggtgt cccattatgc gttttatcag atcactgcat
      541 tactgtagtt gaaccccttt ttgatgtatt gccagaaact tcagcaaaaa gcagttttct
      601 aagaagtggt actacttctc acaatgatac atgctctgaa aaagaaattt atcagagtga
      661 gaatgttttt cgaagaaaca acaagcttga attatacttt aaccctaatc attcacctat
      721 ttatgatccg tactgcaatc aaatctatac acaaacttct ggagcagtat atacccaagc
      781 gcctattact aaatttagta atggggtcct tattttgcat ggagccgtaa cagtaaaagc
      841 taaaattgat agaataatac caggtatttg gcccgcaatt tggatgctac cccaggatac
      901 ggcatttacc gatttggatg gtggtgctag tggtgtttgg ccaaccagtg gagagattga
      961 tacccttgaa atggcaggat acagcggcta ttacaatccc ccagcaactt acttaacaac
     1021 cctccatttc ggccctaccc caggagatga cattcctcga ggttatacga cagtctataa
     1081 aaatgacgcg caacaaatag ttcccttaac cgataaagag catgaattta caatggagtg
     1141 ggatattact ttaaaaaata cggtgaatgg tcaagtcact ttcttgaatc cagcaaatgt
     1201 ggcatcaatt cgtttaagca tgtatcttga tggaaggctt tatcaatcag ataaattaat
     1261 atggaattct tattcaccgc ctgtttgtga taatggactg gtaggcacag gtagatcaga
     1321 tggcacaact tgcggtgttc ctgtatttca aattccttca tcttttgatc ctcttccatt
     1381 aaatgacgct cttccattag aagtatttac cagaggattt aattctggtt ttcgcttaat
     1441 ttcaaatgtg gctagtggag gtcccatttt ttctggtgac ccaattgggg aagctggagc
     1501 tagagaacca gtatcattat tcatctcaga aatttattta caaataataa ataatttggc
     1561 ctttgtaccc aatatattta atggtgcaaa agtttcatgg ctcactccca ccattcccgg
     1621 agagactatt gatcattaca cgcttgaaat ctcagggtat aaatttaata tcaataaaca
     1681 atttccctct acacaaaacc aatttaccat ccccgcccat acactttatg gtaaccgaag
     1741 ttaccatgct gtagtcactg cagtgggtaa tctaggtaca acagtcttag taggtgatgc
     1801 aacagtaaaa attagaccat agtgtttata tattttggag aatcaaatga gtttattgaa
     1861 gtttttagtt tatcccttat cgttaggctt ttctgtttcg tcatttgcag cccaacctta
     1921 cctgggatta caagttggaa gcaatttttt tacacagaaa caaaccatca cagcgtttga
     1981 cgtgactgga gaaataaatc gatggcagtc caatgatgga tttgaaggtg gattgctact
     2041 gggagtccgt tgggtgacca aaccaactta cttattggat gcagagttat ttgccagcta
     2101 caatgcggat gatattagtg taaaaaatac tttaaccggc cctccttttg tagatcatct
     2161 cgatcaaaat ttaggataca gctttggggc tcgtattcta cccgcttatg taatacgacc
     2221 caatttaagc ttgtttgggt tacttggcta tattcaaact caattaaagt ccaactcatt
     2281 ggatggtgtt acagaaggtt tatctggtaa tcaatcagtt atatttaatg gtgtgcaatg
     2341 cggttttgga ataaatattg atttcaacag tggttttaga cttagaatgc aagacagcta
     2401 tgcgttttat ggttcacgca atattactac accatcaatt ggtttagata atcaactgat
     2461 ttcctttaat atacgtcctt atgcaaatca agtctcttta agtttaataa aacaattctc
     2521 ctaaatttgc tatataaatg ggtctttagg aaataattgc ctacaaatat ctgtaggcaa
     2581 ttattttaaa atgcgtaaag tcttttttga agtactgcat ctattaatag ttattaaatt
     2641 ctgtagcgaa caaaaaacta ctgaaccaat aagtgcgcgt gcccaatgaa atagtgcact
     2701 agcctgggtt ttaaagggtt aaaagacaaa aatcggactt gaaacaatcc atcgaattcc
     2761 ccaagagaca tgattttcct acgaagaaaa ggatgtattg ttatagagaa ctatgaactt
     2821 gcccgatgtc gaacgcatat ttcaattgga acaagtgccg tatgcattgt tttgttggca
     2881 ggttgcttgc actcattaaa gtaagaacga tcaatttgac tgaactagca tgtggctttg
     2941 cgagccttac aaacataaaa tcacgttata aacacagcag atggtttttc aaggaattct
     3001 tgatgcattg ccggtcttgt cttaaatgcc ataacctcta tgagtcatat tggcagtgcc
     3061 gttagagttt tttatctcac agtatccaag tgttgtcata gaatgaagga aatatcgcca
     3121 acctcaagat aaggatgaaa aatgggaaat aacatttttg gtcagaaaca attagacgtc
     3181 tatcaaatag tcatagaatt tatcattctt agtaatgaaa ttttggaa
//
LOCUS       DATNSN010000239         3226 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.240, whole genome shotgun sequence.
ACCESSION   DATNSN010000239 DATNSN010000000
VERSION     DATNSN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.240"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..517
                     /locus_tag="VAH76_002981"
     CDS             <1..517
                     /locus_tag="VAH76_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEO1387557.1"
                     /translation="ASSSLQLSPQLFLNAQNIQLKQAEQSKPKETVSCHYKIPEEIIK
                     TDINIILQWAEYAAMQSFHFDYTALEAELKELKSCYTEKGWSEFNAAMHKSGNLNAIT
                     AQRLTMNSLIDGQARLIESQDNQWKIQLPLKVIYKNDRTQVSQFLDIQLTVGRKMNGE
                     LGIVQLIAVLK"
     gene            complement(582..2297)
                     /locus_tag="VAH76_002982"
     CDS             complement(582..2297)
                     /locus_tag="VAH76_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387558.1"
                     /translation="MSDVKPYLSSEMQVKLHFQRMGQLNNPPPLVIQPQNVRLDFIQY
                     CLSQGRNLQSPQATLDNYLKQDKNSMPESEGSREPLLQGDINRKKLSLLFSHLSAFNY
                     NMEGLTIEPVAVADMIKKMQDINQMNDQEVAEYLDKLIGAKIPGHDSMSIDNFLDNIH
                     DLYKNDGVSFHCYLRENFFNPLKADAIKKAEPALNYFMPDLFCQKHINTCELVTTMTI
                     LKKVVTNFNQMQNLCLDSNNLPIEVIEAQKKIKELEQKFENHLKNPSALKEGDIKKLV
                     HDYQKEVEKIQIEMEKSLGKKFNPEITPYLKKYDNHTLSTIHHLVTTRIMPEGFDVDE
                     WALKNTLQAKCTSFQNKLLSDHLPMIIEKDIVKNLHKDTANPEEKKTFLDSLKQMFLY
                     GKSRAFTDFIVCGHALVMDICNNGLLKVLEHRNARKETTVLSHHGLKDLGIQSVHDKH
                     SNPAKEALNSIEQKLKILYMERDNLVKIKAGTQDDSLVHSEISSMLVKKDDEIRDLLE
                     VEKNLKIKYSPEKPKVAHGCFHRIKEEFQKIKTEFIGENKARAKQEALENDRNELNII
                     DKRPT"
     gene            2557..>3226
                     /locus_tag="VAH76_002983"
     CDS             2557..>3226
                     /locus_tag="VAH76_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEO1387559.1"
                     /translation="MTSISEWWMWLGFLAFVLIMLFVDLFVFGGRKAHRVSTKEALSW
                     TILWFTLAIIFNFLLWWYLVENFTFSVANEKALEFFTGYLIEKSLSFDNIFVFLILFE
                     YFAIPAKYQHRVLIYGVIGAIVMRLILILFGIWIVNQFHWILYIFGFFLMITGIKMFI
                     FADKKPELAKNPILHWMRNHLRITQELHGERFFLRKNQLVYVTPLFMVLVLIEVSDLI
                     FAVDS"
BASE COUNT          974 a          531 c          555 g         1166 t
ORIGIN      
        1 ggcctcttct tctcttcagc tatcgcccca gttattcctc aatgctcaaa acattcaact
       61 caaacaagct gagcaatcaa agccaaaaga aacggtttct tgccactata aaatccctga
      121 agaaattatt aaaactgata taaacattat tttacaatgg gccgagtatg ctgctatgca
      181 gtcatttcat ttcgattata cggctctaga ggcggagcta aaggagttaa aatcatgtta
      241 tacggaaaaa ggttggtctg aatttaatgc tgctatgcat aaatcaggta acttaaatgc
      301 aattacagcc cagagactaa ccatgaatag cctaattgat ggccaagcac gattaataga
      361 atctcaagat aatcagtgga aaattcaatt acctttgaaa gtgatatata aaaatgatag
      421 aacacaagtt tctcagtttc ttgatattca attgactgta ggcagaaaaa tgaatggtga
      481 actcggtatc gtgcaactga ttgccgtact gaagtgaaga ataactcata ttgcgctgtc
      541 ttaatgaagt gttatgatca atatgaatgc caataataat gttatgtcgg gcgcttatct
      601 attatattta gttcattacg atcattctcg agtgcttctt gtttagctcg agctttattt
      661 tcaccaataa actctgtttt aattttttga aactcttctt taattcgatg aaagcatcca
      721 tgagcaactt taggtttttc agggctgtac tttattttta aatttttctc aacttccaac
      781 aagtctctta tttcatcgtc ttttttgact agcatagagc taatctcgga atgtactaat
      841 gaatcgtctt gtgttcctgc tttaatttta acaagattat ctctctccat atataaaatt
      901 tttaattttt gctcaatgct attcaacgcc tcctttgctg gattagagtg tttgtcatga
      961 acactttgta ttcccagatc tttcaagcca tgatgagata ataccgttgt ttctttccgg
     1021 gcattccggt gctcaagtac ttttaaaaga ccgttattac atatgtccat aaccaaggca
     1081 tgcccacaaa caataaaatc tgtgaatgcc cttgattttc catacaaaaa catttgtttc
     1141 aaactgtcaa gaaaggtctt tttttcttct ggattagcag tgtctttatg taagtttttt
     1201 actatgtctt tttcaataat cataggtagg tgatcactga gtaatttatt ttgaaatgat
     1261 gtacatttag cttgaagcgt atttttaaga gcccattcat caacatcaaa accttcaggc
     1321 ataattcttg tcgttactaa atgatgaatc gtcgaaagag tatggttatc atattttttc
     1381 aggtaaggtg ttatttctgg attaaatttt ttccccaatg atttttccat ttcaatttga
     1441 attttttcaa cttccttttg atagtcatgc actaattttt taatgtcgcc ctctttaaga
     1501 gctgaggggt ttttaaggtg gttttcgaat ttttgttcta attctttaat tttcttttgt
     1561 gcttcgataa cttctatagg aagattattg ctatccaaac aaaggttttg catttgatta
     1621 aaattagtta caaccttttt taaaatagtc attgttgtaa ccagttcaca ggtattaata
     1681 tgtttctgac aaaataaatc aggcataaaa taattaaggg caggctctgc ttttttaatg
     1741 gcatctgcct taagaggatt aaaaaaattt tcgcgtagat aacaatgaaa acttacgccg
     1801 tcatttttat atagatcatg aatgttatct aaaaaattat caattgacat tgagtcatgg
     1861 ccaggaattt ttgctccaat taatttatct aaatactcag caacttcctg atcattcatt
     1921 tggttaatat cttgcatttt cttgatcata tccgcaacag caaccggttc tatagttaat
     1981 ccttccatgt tgtaattaaa tgcagataaa tgggaaaaaa gcagagataa cttcttacga
     2041 tttatatcac cttgtaataa aggttctctg gaaccttctg attcaggcat cgagttttta
     2101 tcttgcttaa gatagttatc caaggttgct tgaggggatt gcaaatttct gccctgactt
     2161 aggcaatatt gaataaagtc taatcgtacg ttttgtggtt gaataactaa tgggggagga
     2221 ttgtttaatt gtcccatgcg ttgaaaatgc agttttactt gcatttctga agaaagataa
     2281 ggttttacat cactcatatg cacacatcct tgaggaaagc tattcctcat atacaggtaa
     2341 tttaccaaag accatatact ttaaagtata ttaaaaaacg caaaaaatgc acaaaattaa
     2401 tacaattaag ttggtaatta tccctgaatg gcataaatag aggagtcttg attaaaatca
     2461 ttcgccaaca attaagaacc tcaaatctac gagaaaggct ataatgaaaa gacagcaata
     2521 tgatgtaaaa ccatagaaaa aacatggtgg gaattaatga cttcaatcag tgaatggtgg
     2581 atgtggttag gttttttagc atttgtgctc attatgctgt ttgttgattt gtttgtgttt
     2641 gggggaagaa aagctcatcg tgtttccaca aaagaagctt tatcctggac catattgtgg
     2701 ttcactctgg ctattatttt caattttctt ctatggtggt atcttgttga gaattttact
     2761 ttttcagtag ccaatgaaaa agcccttgag ttttttacgg gttatctgat agagaagtct
     2821 ttatcgtttg ataatatttt tgtttttctt attctctttg agtattttgc aataccggct
     2881 aaatatcaac atcgtgttct aatctatggt gtgattggag ccatcgttat gcgcctgatt
     2941 cttattttat tcgggatatg gatagtgaat caatttcatt ggattcttta tatttttgga
     3001 tttttcctga tgataacggg aatcaagatg ttcatttttg ctgataagaa accagaactc
     3061 gcaaaaaacc ccatactaca ttggatgcga aaccatttgc gaattaccca ggaattacat
     3121 ggtgagcgat tttttctcag gaaaaatcag cttgtctatg taacgcctct ttttatggtg
     3181 ttggttctta ttgaagtcag tgatctgatt tttgctgtgg atagta
//
LOCUS       DATNSN010000240         3213 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.241, whole genome shotgun sequence.
ACCESSION   DATNSN010000240 DATNSN010000000
VERSION     DATNSN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.241"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            82..1305
                     /locus_tag="VAH76_002984"
     CDS             82..1305
                     /locus_tag="VAH76_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO1387560.1"
                     /translation="MEFLDFCKAIKESVENYNKDNGILKNSLFLLAELNKLIESHDLN
                     QSLCMTQATILFEGFYRHLSTASLKHEFQIIRANRWAHHFGTPTITFLLSFKTQIDQT
                     VKLFSELTEEVESPLELPWNISKTEGLSIDHDNNIVTNPKSITHVEAPSSRESTGIFT
                     KTHNPFGGFTTTPCDPVSQKFIEHAVLSAKQGGKVLEIGAAFGAATLEAIAKGATVFC
                     NDVEPENLAVVRQRFLEVTDDSSESLTGDSSKLILVPGELPNELIGLPEKQFDAILIC
                     RVLHFFTGAKIEESLALLSKLLTPNGKIYIVCETPYLKNWQRFIPEFNRRVEINEEWP
                     GEITEPAKYESSGRASSLPKFVHWITKEVLDRSLVRSGFTIEHSEYINRKGQFPEDLL
                     LPEYGKESIGAIGMI"
     gene            1316..1810
                     /locus_tag="VAH76_002985"
     CDS             1316..1810
                     /locus_tag="VAH76_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387561.1"
                     /translation="MKQKISWYEWFADMLKEFVAETAKKPQYEIVDIFECKKTGFTKA
                     VIKLSERHTKEKNISDIIMDNELIENLDTKTVRTLTYMATVERLKPDYSIVVQHMTPE
                     VDEYLLEIRSKSKATTIKKSPSELSKDKELIAKFKPEDANKIGYMAGVRETVKEYQLV
                     NKDK"
     gene            1829..2584
                     /locus_tag="VAH76_002986"
     CDS             1829..2584
                     /locus_tag="VAH76_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEO1387562.1"
                     /translation="MRKEIFDALARKKQYKYPYLLLGLYLTFLLSTVCLASRITQIGS
                     MLEPGGIFVFPLTFSICDIVGEVYGYAYPRLFIWIGVLAEFIFSLVVITVSHLPAPEF
                     FTQASAYEIVFDPTLRYVGAGLVGLLIGELTNVYLLSKWKIFLKGKFFIMRSLLSTAL
                     GQALLTIVVDMLNYFGKLTEHHLGWMMVCGYLWKMSCAVLMVFPAWLIVKYLKKVEQV
                     DHYDIHTNFNPFIFGLHEEIPNEYKLNIESVPQ"
     gene            complement(2559..3200)
                     /locus_tag="VAH76_002987"
     CDS             complement(2559..3200)
                     /locus_tag="VAH76_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEO1387563.1"
                     /translation="MADMTLSENLQQLMRIHGNISVSELARLTGIPQPTIHHILAGST
                     KNPRKKALEELSRYFSVSIDELIGQEPLPAVIPDAVKENLQISTIPVIKWESLKEWPS
                     GTARTQDTQEILIDKKIDKNSFALIMPDTSMEPLFQQNSLLIFDSGKAPNDRDFVIVH
                     LSKEGIIAFNRLFIENNTFYLRQGLEDGSLKLIKLDKPHDRILGTLIEARIQY"
BASE COUNT         1037 a          503 c          629 g         1044 t
ORIGIN      
        1 agagatttga ataaaattat tgaaattatt tgatcttaat tatatattcc caaagttttt
       61 taggatgtag gatagcttca aatggaattt ttagattttt gcaaagcgat taaagaatct
      121 gttgaaaatt ataacaaaga taatggtatt ttaaaaaata gcctattttt attagcagag
      181 ttaaataaac tcatcgaaag ccatgatctt aatcaatctc tctgcatgac ccaagcaacc
      241 attttatttg aagggtttta ccgtcatctg agcacagcat ctcttaaaca tgagtttcag
      301 attataagag ctaatcgatg ggctcatcac tttggcacac ccacaattac tttcctttta
      361 tcattcaaaa cacagattga tcaaacagta aaactattta gtgaattaac cgaagaagta
      421 gagtctcctc ttgagcttcc atggaatatt tccaaaactg aggggcttag catagatcac
      481 gataataata tcgttactaa tccaaaaagt attacgcatg tagaagcgcc ctcatccagg
      541 gaaagcacag gaatatttac aaaaacgcat aatccttttg gagggtttac aacaacccct
      601 tgcgatcctg tttctcagaa gttcattgaa catgcagttt tatcagctaa gcaagggggt
      661 aaggtattgg aaataggcgc tgcttttggt gcagcaacat tggaagcaat tgcaaaagga
      721 gcaactgtct tttgtaatga cgttgagcct gaaaatttag ccgttgttcg tcaacgattc
      781 ttggaagtaa cagatgattc cagtgaatcc ttgaccggag atagtagtaa gctaattctt
      841 gttccgggtg aattacctaa tgagctcatt ggtcttcctg aaaaacagtt tgatgctatt
      901 ttgatttgcc gtgttttaca ttttttcact ggtgccaaaa tagaagaatc attggcatta
      961 ttatcaaaat tgttgacccc aaatggcaag atatacatcg tatgtgaaac accgtattta
     1021 aaaaactggc aacgctttat tcctgaattc aatagaaggg ttgaaattaa tgaagaatgg
     1081 cctggtgaaa ttacggagcc agctaaatat gaaagtagtg gacgggcaag ctccttacct
     1141 aagtttgtcc attggatcac aaaggaagta ttagaccgga gtttggtaag atctggtttc
     1201 acaatagaac actcagagta cataaacaga aagggacaat ttcctgagga tttattattg
     1261 ccagagtatg gtaaagagag tattggtgca attggaatga tttgagtaat ggattatgaa
     1321 acagaagatc agttggtatg agtggtttgc cgacatgcta aaagaatttg tggctgaaac
     1381 agcgaaaaag cctcaatatg agattgttga tatttttgag tgtaaaaaaa caggcttcac
     1441 aaaagcagta attaaattgt ctgagaggca tacaaaagaa aaaaatatca gcgatattat
     1501 catggataac gaattaattg agaatctgga tactaaaacc gtacgtactt taacttatat
     1561 ggctactgta gagcgattaa aacctgatta ttctatcgtg gttcagcata tgactcctga
     1621 agtagatgag tatttacttg aaataagatc aaagtctaaa gcaactacta taaaaaaatc
     1681 tccttccgag ctttcaaaag ataaagaatt aatagcaaag tttaaacctg aagatgctaa
     1741 taaaattggt tatatggctg gggttagaga aactgtgaaa gaataccaat tagttaataa
     1801 agataaatag cggtagtaag gagatgtttt gaggaaggaa atttttgacg cattagctcg
     1861 caagaagcaa tataaatacc cttacttatt gcttggtctt tatttaactt ttttactatc
     1921 aacagtgtgt ttagcaagca ggataactca gattggatca atgcttgaac ccggaggtat
     1981 ttttgtattt cctcttactt ttagtatttg tgatattgtt ggtgaggtat atggatatgc
     2041 ataccccaga ttatttattt ggattggtgt tttagctgaa tttatatttt ctttagttgt
     2101 gattactgta tctcatctac ctgcgcccga atttttcaca caagcatcag catatgaaat
     2161 tgtttttgat cctaccctaa gatatgtagg tgcggggctt gttggtttgc ttattggaga
     2221 gctcactaat gtttaccttt tatcaaagtg gaagattttt ctaaagggta aattttttat
     2281 aatgagaagc ctactatcaa cagctttagg gcaagcattg cttacaatag ttgttgatat
     2341 gcttaattat tttggcaagc taacagaaca tcatcttgga tggatgatgg tttgtggtta
     2401 tttatggaaa atgagttgtg cagtgctaat ggtttttcct gcttggttga tcgtgaaata
     2461 tctaaagaag gtggagcaag ttgaccatta cgatattcat acaaacttta atccatttat
     2521 atttggatta catgaagaaa ttcctaatga atacaaactt aatattgaat ccgtgcctca
     2581 ataagtgtcc ctagaattcg atcatgaggt ttgtctaatt ttattagttt gaggcttcca
     2641 tcttctaagc cttgtcgcag ataaaaggta ttgttttcga taaataatct attaaaagcg
     2701 attattccct cttttgataa atgcacgatt acgaaatccc tatcattagg agctttacct
     2761 gaatcaaaga ttaaaagact attttgttga aaaagaggtt ccattgatgt gtcgggcata
     2821 attaacgcga aagagttttt gtcaattttc ttatcaatta atatttcttg tgtgtcttga
     2881 gttctggctg ttcctgaagg ccattctttt aatgactccc atttgatgac aggtatggta
     2941 ctgatctgca ggttctcttt aacagcatca ggaatcacag caggcaaagg ttcttggcca
     3001 attaattcat cgatggaaac agaaaaataa cgggataatt cttctaatgc ttttttccgt
     3061 ggatttttcg tagagccagc tagaatgtga tgaatagtag gttgaggtat gccagttaac
     3121 cgagctaact cactaacaga aatattccca tgtatcctca tgagttgttg caaattctcg
     3181 cttaacgtca tatcagccat taataataac ccg
//
LOCUS       DATNSN010000241         3178 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.242, whole genome shotgun sequence.
ACCESSION   DATNSN010000241 DATNSN010000000
VERSION     DATNSN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.242"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..625
                     /locus_tag="VAH76_002988"
     CDS             <1..625
                     /locus_tag="VAH76_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387564.1"
                     /translation="YNRDFKLISSTTLFFMGLGVGQQNLNIIHNMRFLLLRIMYYLFP
                     FLAAISALYFILYTFHSISSSQEYINPLIVLIPLTTAGIIFFNAYFQDGTIKSDYPSW
                     LKLSLRVYRVILFLLALMMTYKILSDSSLDTNAFIYLLVAVLFSFTYAITAFLNENQE
                     KQWIYMGNIATAIFFIVTLFLCNLPYIPVEFTIGGGNAINFITSTLS"
     gene            complement(691..2190)
                     /locus_tag="VAH76_002989"
     CDS             complement(691..2190)
                     /locus_tag="VAH76_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387565.1"
                     /translation="MKKIVYVLLFISSLTHANCLIYFNEQPLNKRLSDPLYSLLSNTE
                     ICPQSIQELRNSMRLRGLKEVIGMVANRGRNNPVEGSFSFFESVYGRLATDQLIAKGE
                     LFLGYFTRLKDGLIILDQKPRKGKLLIEAISWDAKKALYNFYELRGVEHGGVRWFYRG
                     NSKDAYKDNTWLYRISPEGENHFGSRMRCSACHNSGGPILKEQSLPHNDWWTNQRPLI
                     LLPNKPDGDVSQLLEQVSDASVFANDVITGMRHLSDSKKMNAFQGKLSLQEQLRPLFC
                     TTEINLQSSTDSSETVVSIPSAFWLNPLLGDINLTISAEDYNQLLKEFGIRFPETSLQ
                     DADHAWLTPVKAEADLNAIKQLIQQNIISKKFAQSVLMIDFTNPLFSSKRCELLKLIP
                     ESYQTNSKEWVEQFLINLRSNEEQFKGARQLADYMSKEQLDSKELMKKIKIYEYFLNQ
                     SISTRTGLRKHFQHLIELRKAVFRSELSQNPLGQILEPGFRVIFPEIQP"
     gene            complement(2333..3052)
                     /locus_tag="VAH76_002990"
     CDS             complement(2333..3052)
                     /locus_tag="VAH76_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387566.1"
                     /translation="MTERKKTAVKNKKAPVKKSQLTVSLLIFIDLISILLSLMPGVAA
                     FSLTGLFLYYGFSKTWLVLICSPFVLLFSFILIIALIRISLPKLKPGRYKRELNKTMI
                     AWFCHFALSRAAKIVGLIHLLQSFNVIKYLYWRALGIKVSFRVMNSFDIDFVDCPLIS
                     IGKDVTLASQVSISCHSDVGNYLFLSPVVIEDNVFVGMTTKIGPGTTIKKGAWIGYGN
                     AFVNQVIEENAKIGNVRPVPE"
BASE COUNT         1005 a          642 c          520 g         1011 t
ORIGIN      
        1 ttataatcgt gattttaaat taatcagtag tacaacctta ttttttatgg gtttgggtgt
       61 tggtcaacaa aatctcaata tcattcataa catgcgattt ttattactta gaataatgta
      121 ttatctgttt cccttcttgg cagcaataag tgccctctat tttatattat atacatttca
      181 ttctatttcg agcagtcagg aatacatcaa cccgttaata gttctaattc cactgactac
      241 agcaggtatc atatttttca atgcctattt tcaggatgga actattaaat cagactatcc
      301 ttcctggtta aaattatctt taagagtcta tcgagtcatt ctgttccttc tggctttaat
      361 gatgacttat aagatccttt ccgactcctc actagataca aatgctttca tttacttact
      421 ggttgccgta ctattcagtt tcacttatgc cattacagca tttttgaatg aaaaccagga
      481 aaagcaatgg atttatatgg gaaatatagc tacggcgata tttttcattg tcaccctgtt
      541 tctctgtaat ctgccctata ttcctgttga gttcacaatt ggtggaggaa atgcaatcaa
      601 tttcatcacc tcaactcttt cgtagaaaat aatactcagc gcatggcagt tttttgtcat
      661 gcacagagcc aataatagac ctcttcttgt ctacggctga atttcaggga aaattacccg
      721 aaaccctggc tctaaaattt gccctaaggg gttttgtgac agttcactac gaaagaccgc
      781 ttttcgcagc tcgattaaat gctgaaaatg cttccttaat cctgttctgg ttgatataga
      841 ctggtttaga aaatattcat aaattttgat ttttttcatc agctcttttg aatccaattg
      901 ctctttactc atataatcag ctagctgacg tgccccttta aattgctcct cattgcttct
      961 taaattaatc agaaattgtt caacccattc tttgctgttc gtttgatagg attccggtat
     1021 taattttaat aactcacatc gttttgatga aaacaaagga ttggtaaaat cgatcatcag
     1081 cacggattgt gcaaattttt tgctaattat attctgttgg ataagttgct taatcgcgtt
     1141 gagatcagcc tccgccttga caggtgtaag ccaggcatga tcagcgtctt gcaaagacgt
     1201 ttctgggaaa cgaatgccaa actctttcaa taattgatta taatcttcag ctgaaatggt
     1261 caagttaatg tcgcctaata gtggattaag ccaaaacgct gatggaatgg atacaacggt
     1321 ttctgaagag tcagtgctgg actgaagatt tatttcagta gtacaaaata aaggccgtaa
     1381 ctgctcctgc aatgacagtt tcccttgaaa tgcattcatt tttttactat cgctcaaatg
     1441 tcgcattcct gttatcacgt catttgcaaa tacactggca tcactgactt gttccaataa
     1501 ttggctcaca tctccatcag gcttattggg gagcaaaata agcggacgtt gattagtcca
     1561 ccaatcatta tgaggaaggg actgttcctt aaggataggc ccgccggagt tgtggcaagc
     1621 tgaacatcgc atacgactgc caaaatgatt ttcaccctct ggacttattc tatataacca
     1681 ggtgttatct ttataagcat ccttcgagtt tcctcgataa aaccaacgta cgcctccatg
     1741 ttctactcct cgcaattcat aaaaattata aagcgccttt ttagcatccc agctgattgc
     1801 ttcaatcaat aatttacctt tcctgggttt ttgatccaaa ataattaggc catcttttaa
     1861 acgagtaaag taccccagaa ataactcccc ctttgcaatc aattggtcag ttgctaatcg
     1921 accgtacact gattcaaaaa aactgaaact gccttcaaca ggattatttc ttcctcgatt
     1981 agcgaccatc cctatgactt cttttaatcc tctcaatctc attgaatttc ttaactcctg
     2041 aatcgattgc gggcaaattt cagtattgga caacagagaa tacaaaggat cagataacct
     2101 tttattaagc ggttgttcgt taaaatagat cagacaattg gcatgagtca gcgaagaaat
     2161 aaataacaag acgtaaacaa tttttttcat tctggaacat ccgtttaatc aaagaaataa
     2221 tcaacagctt caaagcctga ttgcagcaat ctggattcat taagttaacc gggaaaacaa
     2281 tgcactaaat tctgattttt attaatccaa acttaagata aaacatttca ttctattcag
     2341 gaacaggtct aacattgcca atttttgcat tttcctcaat aacctggtta acaaaagcat
     2401 tcccataacc tatccaggcc ccttttttaa ttgttgttcc tgggccaatt ttagtcgtca
     2461 ttccaacaaa aacattatcc tctatcacca caggcgataa aaaaagataa tttcctacat
     2521 cagaatgaca actgatagag acttgactgg caagggtaac atcctttcca atcgaaatga
     2581 gagggcaatc cacaaaatcg atatcaaatg agttcataac gcgaaatgac actttaattc
     2641 ccaatgcacg ccaatacaaa tatttaataa cattaaagga ttgtaataaa tgaatcagac
     2701 caacaatttt cgctgcacga gagagtgcaa aatgacaaaa ccaggcgatc atggtcttat
     2761 ttaactctcg cttatagcga cctggcttaa gttttggaag gcttatcctg ataagtgcaa
     2821 tgataagaat aaaagaaaaa agtaatacaa acggtgagca aattaaaaca agccaggttt
     2881 ttgaaaaccc ataatacaaa aacaaaccgg ttaagctaaa tgcggccacg ccaggcatta
     2941 aactgagcaa tatagaaatc aaatcaataa aaatcaataa agaaacagtc agctgacttt
     3001 ttttgacagg tgctttttta ttcttgactg ccgttttctt gcgctccgtc atcctgtgat
     3061 ttccttgaaa ataatagaca attcatttgc caacctttga ggttgtttta tttaaacctg
     3121 gaggtatact aacaaaaaca agcaaaagat tggaagatac attatcaaat tcaaagag
//
LOCUS       DATNSN010000242         3146 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.243, whole genome shotgun sequence.
ACCESSION   DATNSN010000242 DATNSN010000000
VERSION     DATNSN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.243"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            211..1455
                     /locus_tag="VAH76_002991"
     CDS             211..1455
                     /locus_tag="VAH76_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEO1387567.1"
                     /translation="MSDLKNLILNSSILLDTSSTVQNDLNPEQLINVHEDSDGGRDLL
                     TPESFILLMAEILSTNNADTICEYEHQNSADNKVLQNLQFKEVEEDEQIEGLNEQKKS
                     LDNPLWHDVDLPVINEISGNQCEGQFESTLEKNVALTWINSENFQPPIVKPENQTDET
                     YKIWDSEITSIESGKLKLDFPKADYDGNNINPYQKEAINQPGNDSSKNHQLIKGNDSF
                     PILEDIKTNAEPGTKAIKSEVEATKVGTEIPGSSFQMNQKAGSDIQPPVVSNSHIPKN
                     FSIPIHVNHSQWSEQLSEHIVWLGHQDVKSALIKIHPEELGPLEINVKVVKDNASVNI
                     STHSVYVKEIVDQAIPRLREMMAQQGINLSEVHIGTDTSSRQGSQKNHHAEIDLIQSA
                     EDKNKITSLTRHLPKGLVDYFA"
     gene            1492..2694
                     /gene="mavA"
                     /locus_tag="VAH76_002992"
     CDS             1492..2694
                     /gene="mavA"
                     /locus_tag="VAH76_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavA"
                     /protein_id="HEO1387568.1"
                     /translation="MSHNYFRTCLTTKDNKFFWQKPLASERFDKLIADRKFQKSIAHS
                     RKNQLMYSFIESKIASDYALLIDTKLREQLKQFTLDDFNDISGFERKEKSNSRQQSYF
                     KLRQELEFFLKNDIQQHKSCPDAQLNAFRRWINISDLLLRHHCYEGFLLVFVNLQLIA
                     DKQLIDGLPVSVRNNYNQLCQLSSPIGNHGALRHFMNTHQGESDFTPLFFTYHAIGAL
                     DESLESLKDKEVLLKKQLKHLNKKISHLRTEATPGVIDVISELLKNKQQIPKKMMERR
                     GHLIQLMEEVRFTGKQLKQIQVNMQNQLEQRANLVGLIVKEQKATPRTLPEYFEKTYS
                     IIKYRFNRQSIATVKSSQLPESTASSNPSSSNLYKSKLLPHFWNRHGKSSSSYWEEVF
                     TPSCLNNK"
     gene            complement(2745..3062)
                     /locus_tag="VAH76_002993"
     CDS             complement(2745..3062)
                     /locus_tag="VAH76_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387569.1"
                     /translation="MKIVSAYVLRLFAILNLTFLLFITISFTNVASASNTNADQYLSG
                     NTQLAYFIGVHSYPGNYYWRGPVYYKNAYWTKWRYIGYGCQKSCLIDRWSGRVIRCAK
                     RCF"
BASE COUNT         1136 a          528 c          557 g          925 t
ORIGIN      
        1 atggcagctg aataaatagt gatacatgat aaatttctct ccctataaaa tatgtcaaaa
       61 aactagcatt atttttttag gggttatagt gagtattaag aggttttaca tgcatgcaat
      121 attttttaac tgtcccttga ttattttttc agtaagcttg cagatggtat gagttttgct
      181 atatgttaat cataacacag ggagtgtgat atgtctgatc tgaaaaacct tatactcaat
      241 tcatccatct tattggatac ctcttcaaca gttcaaaatg atctaaatcc tgagcaattg
      301 attaatgttc atgaagattc tgatggtggc agggatttat taactcctga atcttttata
      361 ctgctaatgg ccgaaatatt atcaacaaat aatgcagata caatatgcga atatgagcat
      421 caaaattcag cagataataa agttttgcaa aatttgcaat ttaaagaggt ggaagaggac
      481 gaacaaattg aaggactcaa tgagcaaaaa aaatccttag ataatccatt gtggcatgat
      541 gttgatttac ctgttattaa tgaaatatca ggtaatcaat gtgaagggca gtttgaatca
      601 acactagaaa aaaatgtggc tttgacatgg attaattctg aaaatttcca accaccaata
      661 gttaaacctg aaaatcagac agatgagact tacaaaattt gggactcgga aataacttct
      721 attgaatctg gcaaactgaa actggatttt ccaaaagcag attacgatgg gaataacatt
      781 aacccttatc aaaaagaggc aatcaatcaa ccggggaatg attcatcgaa aaatcatcaa
      841 ttaattaaag ggaatgactc tttcccgata ttagaagaca ttaaaacgaa tgctgagcca
      901 ggaactaaag caataaaatc tgaagtagag gcaactaaag tgggcactga aatacctggt
      961 agttcctttc aaatgaacca aaaagccggt tcagatattc aacccccagt tgtttccaac
     1021 tctcacatac ctaaaaattt ttcaattcca attcatgtca atcattcaca atggtcagag
     1081 caattatctg aacatattgt ttggcttggt catcaagatg taaaaagtgc cttaattaaa
     1141 attcatcctg aagaattggg gcctttggaa attaatgtaa aagttgtaaa ggataatgcc
     1201 tctgttaaca tatctactca cagtgtttat gtaaaagaaa ttgttgatca agcgattccc
     1261 agattaagag agatgatggc tcagcaaggt ataaacttgt ctgaggtgca tattggaacc
     1321 gatacaagtt cccgccaagg ttcgcagaaa aaccatcatg ctgaaataga tcttatccaa
     1381 tcagcagaag ataaaaataa aatcacttca ttaaccaggc atctacccaa aggactggtc
     1441 gactactttg cttagtttaa caaatcaatt gttggtaagg agtttacaac agtgtcccat
     1501 aattattttc gtacctgtct gacgacaaag gacaataagt ttttttggca aaaacctttg
     1561 gcatcagaac ggtttgacaa gctgattgct gatagaaaat ttcaaaaatc aatagctcac
     1621 agcaggaaaa atcaattaat gtatagtttt atagagtcta aaattgcctc agattacgca
     1681 ctattgattg atactaaatt aagagagcaa ttgaaacagt ttactctgga tgattttaat
     1741 gatatttcag gatttgaacg aaaagaaaaa tcgaattcca ggcaacaaag ctatttcaaa
     1801 ttaaggcaag agctggaatt ttttcttaaa aatgacattc aacagcataa atcttgccct
     1861 gacgctcaat taaatgcgtt cagacgctgg ataaatatat cggatttact cttaagacat
     1921 cattgttatg aaggctttct tttggtattt gttaatttgc aattaattgc agacaaacag
     1981 ttaatcgatg gcttacctgt aagcgtaaga aataattata atcaactatg tcaattaagt
     2041 tcaccaatag ggaatcatgg cgctttgcgc cattttatga atacgcatca gggtgagagt
     2101 gattttactc ctttattttt tacctatcat gccattggag ctttggatga atcgcttgaa
     2161 tccttaaagg acaaggaagt tcttttaaaa aagcaattaa aacatctgaa taaaaaaatt
     2221 agtcatttaa gaacagaagc cactccagga gtgattgatg tcatttctga attattgaaa
     2281 aataagcaac aaataccaaa aaaaatgatg gagcgtagag gacatctgat acagctgatg
     2341 gaagaggtta gattcactgg aaaacaatta aaacaaatac aagttaacat gcaaaaccaa
     2401 cttgaacaac gcgctaattt ggtaggattg atagtcaaag aacaaaaagc aacgccaaga
     2461 acgctccctg agtatttcga aaaaacctat agtattatca agtacagatt caataggcag
     2521 tctattgcta cagtcaaaag ctctcagctt ccagaatcaa cagcaagcag caatccaagt
     2581 tcctcaaatt tgtataaaag taaattgctc ccccattttt ggaatagaca tggaaaatct
     2641 tcctccagtt actgggaaga ggtattcacg ccaagttgtt tgaacaataa ataagagaat
     2701 tagatgaact ctgcttcata caagccctgt ttatatgaat ttggttaaaa acaacgtttg
     2761 gcacagcgaa tgactcgacc actccatcgg tctatcaggc agcttttctg gcaaccataa
     2821 ccaatatatc tccattttgt ccagtaagcg tttttgtaat atacggggcc gcgccagtag
     2881 tagttaccag ggtagctgtg aaccccaatg aagtaagcaa gttgagtatt tccggaaaga
     2941 tattgatcag cattagtgtt gctggcactt gctacattag taaatgatat agtaatgaac
     3001 aatagaaaag ttaagttcag aatagcgaat aatctcagga cataagcact tactattttc
     3061 atgataaatt ccttttatcg cagataatat tcaaattata gattaataat ataacaaatt
     3121 gtctcataat attgcaaaat tgcttt
//
LOCUS       DATNSN010000243         3121 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.244, whole genome shotgun sequence.
ACCESSION   DATNSN010000243 DATNSN010000000
VERSION     DATNSN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.244"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            166..1890
                     /gene="pilB"
                     /locus_tag="VAH76_002994"
     CDS             166..1890
                     /gene="pilB"
                     /locus_tag="VAH76_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEO1387570.1"
                     /translation="MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQ
                     YIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIPVNLVNEKLIKRHAMVPLFSRG
                     TNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
                     DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYERE
                     YRIRYRQDGILHEVATPPASLSSRITARIKVMSNLDISERRIPQDGGFKMKISKSRAI
                     DFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGP
                     TGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFL
                     RQDPDIIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIA
                     SSVTLIIAQRLARKLCNQCKAVRDDFTNQGLIELGFKEADLVNLKLYKAVGCEQCTSG
                     YRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEGITTIE
                     EVNRVTVD"
     gene            1896..3116
                     /locus_tag="VAH76_002995"
     CDS             1896..3116
                     /locus_tag="VAH76_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO1387571.1"
                     /translation="MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGI
                     VTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMIESGIPLVQAFDIVAKGQSNKRL
                     KDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
                     IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITM
                     SEFMQAYWYIIFGALGGIVYSFFYAKNHSLEFAQTIDRVMLKFPVIGPILEKAAIARF
                     ARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATGQQMFIAMENTHLFP
                     NMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLV
                     VAMYLPIFKLGSAV"
BASE COUNT         1023 a          508 c          670 g          920 t
ORIGIN      
        1 aagagagttg cgttatagcc cgtgtttgca aaaaattgat gtggcaaagt aaccagaaat
       61 aggatttagg ggcagttcca aaataccggg ttttgttcaa aaaaccatgc aattataaaa
      121 aaatttaact atatttaaaa ctaagataca ctttgaataa atgaaatggc tctagctaca
      181 gaagaatata gattacaggg aatcggccag cttctcgtgc tggaaaagct gctagacaaa
      241 acaaaagcaa ttgaactaca caagttggca gcagcagaaa agatatcttt gctgcaatac
      301 attgtaaaaa ataaaatatt atctgctgaa caaattgcac tgacggctgc tcaaaatttt
      361 ggcgtaccca tgttggatat taattgcatt gatgtgggca ccattcctgt caatctggtt
      421 aatgagaaat taattaaacg tcatgccatg gttcctcttt ttagtcgcgg caccaattta
      481 tatctcgcaa cagatgatcc tagtaaacag gcttcattaa aggaaattca atttcatacc
      541 ggattaaata ctcatgcgat agttgtagaa acagataaac ttagtgctct gattgataac
      601 ctgttaacag caaaggaaag tcagggctta tcagagtatg ttgaagactc tggagatcta
      661 gaaggtttgg aaataagtgc tgatgatgaa gatcaagata ttgatactgc aacatcagta
      721 actgatgatg cacctatcgt aaaattcgtc aataaaattt tattggatgc gataaggcaa
      781 ggagcttctg atatacactt tgagccttat gaacgagaat atcgaatcag gtacaggcaa
      841 gatggtattt tacatgaggt cgctacccct cccgcaagct tgtcatctcg tattacagca
      901 cgtatcaagg taatgtctaa tttggatata tcagaacggc gtatccccca agatggtggc
      961 ttcaaaatga aaatttccaa gtcacgagca attgatttca gagtgagcac atgtccaacc
     1021 tcggctgggg aaaaagtggt tatgcgggtt cttgattctg gtgctgctaa attaggtatt
     1081 gaagcattag gatttaatcc tgttcagcga acaaatttcc tgaaagcaat ccagcgccca
     1141 caagggatga ttttagtcac tggccctact ggtagtggta aaacagtcac tttatatacg
     1201 gctttaaata tattaaatac aattgaggtt aatatttcaa cagctgaaga tccagttgaa
     1261 atcaaagtgc ctggtatcaa tcaggttaat attaatccta aagctggcct aaccttttcc
     1321 ggtgcattgc gttcgtttct aaggcaagat cctgatatta tcatggttgg tgagatacga
     1381 gaccttgaaa cagcagagat tgctataaaa gctgctcaga cagggcattt ggttctttct
     1441 actctgcaca caaacagtgc cgctgaaact ttaaatcgtt tagtaaatat gggaatacct
     1501 acttttaaca tcgcgagttc ggttacctta attattgcgc aaaggctggc aagaaaatta
     1561 tgcaatcaat gtaaggcagt gcgtgatgac tttaccaatc aaggcttgat tgaactaggt
     1621 ttcaaggaag ctgatttggt taatcttaaa ttatacaaag cagttggttg tgagcaatgt
     1681 acaagtggtt atcgcgggcg agttgggttg tttgaggttt taccgatgac aaaagagctt
     1741 ggccaattga ttatgtcagg tggtaattcg ttggatatat taaagttagc tcaatcagaa
     1801 ggtatgctga ctatctttca atcgggtata gaaaaagtta aggaaggaat cacgaccata
     1861 gaggaggtca atcgggtaac cgttgattaa tttggatgga taaaaatagc ccacccctat
     1921 taacctttca ttatcaaggc attaataaag ccggtcaaaa aatggagggt gatattcaag
     1981 ccagaagctt ggcaatagct aaagctgatt tgcgtaagca ggggattgtt accaataaag
     2041 tagtaaaaaa aagaaaacct ttgtttgata gaaaaaataa aaaaatcaca caagcagata
     2101 ttactgtatt tagccgccaa ttagcgacga tgatcgagtc tggaatacca ttagtgcaag
     2161 cgtttgatat tgttgccaaa ggacaaagca ataaaagact taaagactta atagaaacaa
     2221 ttaagcacga tatcgaaacg gggctaacgc tagcagaatc cttaattaaa catccactct
     2281 attttaatga attattttgt aatcttgtcg atgcaggtga aaaatcaggc tcattagata
     2341 tcatgcttga caaggttgca acgtataaag aaaaaattga aatcataaaa aagaaaataa
     2401 aaaaagctct cacttatcca attgctgtta tggttgtcgc acttttggtt actgcgggat
     2461 tattaatcta tgtggttcct caatttgaat cattgtttaa aggatttgga gctgacttgc
     2521 cagcgatgac tagagctgtg atcactatgt ctgaatttat gcaggcttat tggtatatta
     2581 tttttggagc tttgggtggg attgtctata gcttttttta tgctaaaaat cactctttgg
     2641 aatttgctca aactattgac agagtgatgc taaaattccc tgtaattggc cctattttag
     2701 agaaagcagc aattgctcga tttgcgagga cattatccat tacttttgct gctggattac
     2761 ccctggttga agcattgaaa tctgtagctg gagcgaccgg gaatattatt tatgccaagg
     2821 cgactgataa aatcagggaa gaagttgcaa caggacagca aatgtttatt gctatggaaa
     2881 acactcattt atttccgaat atggtaattc aaatggtagc aattggtgag gaatcaggtg
     2941 cccttgaaaa aatgttaagc aaagtagctg atttttatga ggaagaagta aataatgctg
     3001 tagatgccct aagtagttta ttggagccga taattatgtc aatattgggt atactggttg
     3061 ggggattggt ggtggctatg tatttaccta ttttcaaact gggttctgct gtttaaacag
     3121 a
//
LOCUS       DATNSN010000244         3121 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.245, whole genome shotgun sequence.
ACCESSION   DATNSN010000244 DATNSN010000000
VERSION     DATNSN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.245"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            309..977
                     /locus_tag="VAH76_002996"
     CDS             309..977
                     /locus_tag="VAH76_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEO1387572.1"
                     /translation="MCGRFAYIASYDKLKYQFHLANAIEIPPRFNISPGADVVCLVET
                     VGHEIQCVLLRWGLIPSWTTDRKKLGNLINARAETVFEKPTFRQAIKSKRCLIPMSGF
                     YEWHQEDGVKQPYFFQKKNHDLLAVAAIRDTWQQNEEVIHSCCLITTDANAWMQPVHN
                     RMPVILGEEAQAIWLNNTQCDKAQLMALMKPYPYEDLEGYRVTNLVNKANFDHPLAME
                     PLSE"
     gene            1112..1618
                     /gene="umuD"
                     /locus_tag="VAH76_002997"
     CDS             1112..1618
                     /gene="umuD"
                     /locus_tag="VAH76_002997"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEO1387573.1"
                     /translation="MSPRGGKRVGAGRPRGEPTKAVRISLSQLAELERLKSHVSYQLP
                     VFASKIQAGFPSPADDYIEGYLDLNTKFIKHPSSTFVLQATGESMVDAGIFSGDWLLV
                     DRSIEPSDGRIVIAAVNGELTVKRLSKKGGRVQLLPANPKFQPIDITEDSEMVIWGVV
                     TLVLHELA"
     gene            1605..2882
                     /locus_tag="VAH76_002998"
     CDS             1605..2882
                     /locus_tag="VAH76_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEO1387574.1"
                     /translation="MSLPEMFALIDCNNFYASCERLFRPDLKDVPIVVLSNNDGCCIA
                     RSNEAKALGIAMGEPYFKIKHLCKQHGVKAFSSNYTLYGNMSHRVMCTIEEAWPHIEI
                     YSIDEAFLDLRSLPVNSHDLFCEQLQKKILKYTGIPTSIGIGPTKTLAKAANHLCKKV
                     YKIPVFNITSSRERLLQQISVGDIWGVGRQWANKLISRGIQTAYDLAMTNPHLLKKCF
                     NVVLMRTAMELQGIACGGLEAIEPKQSIMSSKSFGQMQTQLASIEESISSHCARAVEK
                     MRRQQLVAKRLVVFVHTNRFREDLAQHFQSIEFKLINPTDDLRLITKMAKRCLQRIFK
                     PWYYYKKAGVCLEDLIPKNPRQLDMFHQPSDEHLNHTEQLMAVFDQINQKYGRSTIRL
                     AAEGYSKPWAMRAELKSPAYTTRWSDLPRAIIR"
BASE COUNT          911 a          586 c          676 g          948 t
ORIGIN      
        1 ataagatgat taggcctaaa ttcttttttc ttatcccttt tctgtggata tcttattata
       61 ccaaataatg actgaataat cagtaaccta taagagccct tgaccccatt gatttatatc
      121 attttttaat gcctgttatt tcattttagt tatccacatt ttctgtggat aagtctgtta
      181 gtagtttgtc aaacttcttg tgggatttac aagttaatcg gatgattcat cctttaccaa
      241 agtgtgtttc gttaatgggc aaagaaatat gaagcagtgc tacacttaaa aaaacagctg
      301 agaatgatat gtgcggtcga tttgcttata ttgcttctta tgacaagcta aaataccaat
      361 ttcatctagc caatgcgatt gaaattcctc cgcgctttaa tatttctcca ggagctgatg
      421 tagtatgtct tgttgaaacc gttggtcatg aaattcaatg cgtattattg cgttggggat
      481 tgattccttc ctggacaacg gacagaaaaa aattaggaaa tctcatcaat gcacgcgccg
      541 aaacagtttt tgaaaagccg acttttcgtc aggcaataaa atcgaaacgc tgtttaatcc
      601 ctatgagtgg cttttatgaa tggcaccaag aggatggtgt aaaacagcct tatttttttc
      661 aaaaaaagaa tcatgatttg ctggctgttg ctgctattcg agatacctgg cagcagaacg
      721 aagaggtgat tcattcctgt tgcctgatta ctacggatgc taatgcatgg atgcaaccag
      781 tgcacaatcg tatgccggtg attttaggtg aagaggctca agccatttgg cttaacaaca
      841 cgcaatgtga taaagcacaa ttaatggcgt taatgaaacc ttatccttat gaggatttgg
      901 aagggtatcg agtgactaat ttagtgaaca aagccaattt tgaccatcca ttggcgatgg
      961 agccattatc tgaataaaaa atttaaaaat aattttctta atattctcga agacttagtg
     1021 ttgatttcaa ctcatgttgt tctgaggcgg gatttacagc ttatttttcc ttgtctatga
     1081 tatttgtacg taaatcaaaa aaagaatcat catgtcacca cgaggcggaa aaagagtagg
     1141 ggcgggtcgc ccaaggggtg agcctaccaa agcagttcgt atatcattat ctcaacttgc
     1201 tgaattagag cgtctaaaga gtcatgtatc gtatcaattg ccggtatttg ctagcaaaat
     1261 tcaagcagga tttccatcac cggccgatga ttacatcgag gggtatcttg atttaaacac
     1321 caaatttatt aaacacccat cgtctacctt tgttttgcaa gccacagggg agtctatggt
     1381 ggatgctgga attttttctg gcgactggct gttagtcgat agaagtatag aaccttcgga
     1441 tggacgcatt gtcattgcag ctgtgaacgg tgagcttaca gttaaacgcc tttcgaaaaa
     1501 agggggaagg gtgcaattac ttcctgctaa tcccaaattt caacccattg acattacaga
     1561 ggacagtgag atggtgattt ggggagtggt gactttggtt ttacatgagc ttgcctgaga
     1621 tgtttgccct tatcgactgc aacaattttt acgccagttg tgagcgtttg tttcgtcctg
     1681 atttaaagga tgtccccatc gtggtgctat ccaataacga cggctgttgt atcgcacgct
     1741 cgaatgaagc caaagcattg ggcattgcca tgggcgagcc atacttcaaa attaaacatt
     1801 tgtgcaaaca gcatggcgtg aaagcttttt cctcaaatta tacgttgtat ggtaacatga
     1861 gtcatcgcgt gatgtgcact attgaagaag cctggcctca tatcgaaatt tactcgattg
     1921 atgaagcgtt tcttgattta aggagtttac cggttaactc gcatgatttg ttttgcgagc
     1981 agttacaaaa gaaaatcttg aagtacacgg gaatacccac ttccatcggc attgggccga
     2041 ccaaaacact agccaaagcc gccaatcatt tatgcaaaaa agtttataaa atccctgtgt
     2101 ttaatatcac ttcgagtcgt gagcggttat tacaacagat ttccgttggg gacatttggg
     2161 gagtagggcg gcaatgggcc aataaattaa tttcgcgagg tattcagacg gcttatgatt
     2221 tggcaatgac caatcctcac cttctgaaga aatgttttaa cgtcgtgttg atgcgtaccg
     2281 ccatggagct tcaaggaatt gcttgtggcg gtttagaggc aatagagcct aagcaaagta
     2341 ttatgtcatc taaaagtttt ggtcagatgc aaactcaact tgcttcgatt gaggaatcaa
     2401 tcagtagcca ttgtgcccgt gcggtggaga aaatgcgtcg ccagcaattg gtggcgaagc
     2461 gcctggttgt atttgtgcat acgaaccgat ttcgcgaaga tttggcacag cactttcagt
     2521 ccatcgaatt taagctgatt aatcctacag atgatttgcg cttaattacc aaaatggcca
     2581 agcgatgtct gcaacgcatt tttaaaccat ggtattacta taaaaaggca ggagtatgtc
     2641 ttgaggactt aattcccaaa aacccacgac agctggatat gtttcatcaa ccaagtgacg
     2701 agcatctaaa ccacacggaa caattgatgg cggtctttga ccaaatcaat caaaaatacg
     2761 gacgaagtac aatccgcctc gcggcagagg gttattcaaa accttgggcg atgcgtgctg
     2821 aactgaaatc gcctgcctat accacacgat ggtctgattt acctcgtgca atcattaggt
     2881 aattataaat agggcaatta ataacctaat tttgatacgc ctgtgggtgt ttttaattag
     2941 cttaaatcag tcagatgtat ctcaaattac ttaattttta aaagtatgta tggtttcatt
     3001 ttgctggggt tttgttttat tcataaccat aatgattgca aattactcca gatgagattg
     3061 atgaaatcac agtaatgatt gttagcgatt cagatcgaaa atagcatcga ttgtgctagc
     3121 a
//
LOCUS       DATNSN010000245         3114 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.246, whole genome shotgun sequence.
ACCESSION   DATNSN010000245 DATNSN010000000
VERSION     DATNSN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.246"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..1751
                     /locus_tag="VAH76_002999"
     CDS             51..1751
                     /locus_tag="VAH76_002999"
                     /inference="COORDINATES: protein motif:HMM:NF012451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEO1387575.1"
                     /translation="MSIFYIFSNQLTDINNPQIQNIKKGRIFDSEHKMNEFRYAIASY
                     DSDKKGWVLFKVEYPDKSALEHALNTGKEVDLSYLTFNNIEFSIASLFATTPFKSLQA
                     EEKEGKSNREEASIKKTLAEIERLENQINETHAQFIKDNIENNEVFNFFKDKSYIFEA
                     AMQNYDVAKVFMSSSEVMSLLSPKQAIAVLFKHRANIGQQFSLFDADNPRFISKDNPT
                     IIEGLKSDSFEAKRFINSRLGALLSPLEFAKILLTHQDNSSFFIQTLARHEYAFSEES
                     LKDPQVKEIYQQLLIKATDPVNARAYKKYFIQHPAHIQLLFNFLMATENANAAIAFMN
                     SYENMSLFSVKQVTELLLNHRAHLKLQNVPLFTPHNPQLIALDNPMLINALKNDPGIA
                     IQFINSRLINLFTPLTAAKIMLAHKDNDRFYHQTRPRHASLFTSENLNDPRIKEIYDQ
                     YFHNRNQRKNRFFAQQPQVNEAEEYFRFANRDEIKFNPYKVLGVNEDASLEEIKKAYK
                     KLAIAWHPDRVQAGENIEARTERFKKLGVAYNILIDPAKRQEYDNRPQNRFGRHNPMH
                     "
     gene            1702..2091
                     /locus_tag="VAH76_003000"
     CDS             1702..2091
                     /locus_tag="VAH76_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387576.1"
                     /translation="MTIGLKTDSEGIIPCIKGFVIGLAIAIASKKGDTIQPGGAQLAN
                     QLSFADQVSATPSYIVSGVSRQKTIANYPIKFSHSKFMNNIPLNPNVIKVLNALNHIG
                     KNHITEDMVQKSKKILSQRDKAQLKKY"
     gene            2147..2326
                     /locus_tag="VAH76_003001"
     CDS             2147..2326
                     /locus_tag="VAH76_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387577.1"
                     /translation="MQDLLEFSSKERQQAFEEATARSTTIKNPITIEKDFWVCWTPDQ
                     IFSNEDLSPHVVFIC"
     gene            2512..3111
                     /locus_tag="VAH76_003002"
     CDS             2512..3111
                     /locus_tag="VAH76_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387578.1"
                     /translation="MFLKYLIVIMFLMTSSLLFAQLPKEIVVACHEGETPNDSVSYMY
                     LPGNLRILVPISKTLTNSSISYTPLPGSFDYKKIKGSEERYEFDVNMRHFSIEDNQYF
                     VRDNSWIKLSDAINHSNHPIAEPGNINNPMMVAKITFNKKDYLCISGSLCVNGSLAAV
                     GEYYIVENAFDYDKKLQLYYYFFDKEFVEMVRIRDNWNG"
BASE COUNT         1103 a          517 c          520 g          974 t
ORIGIN      
        1 attgttaatt tcaatagaat tggcccccat tttatcatca tgacctacta atgagcatat
       61 tttatatttt ttcaaatcaa ctcactgata tcaacaatcc tcaaattcaa aacattaaaa
      121 aaggtagaat atttgattct gagcataaga tgaatgagtt taggtacgct attgcttctt
      181 atgattcaga taaaaaaggt tgggtgctat ttaaagtgga gtatcctgat aaatctgctc
      241 tggagcatgc attaaacaca ggaaaagagg tggatttaag ttatttaact tttaataaca
      301 ttgaattctc aatagcatcc ttgtttgcta caactccgtt taaaagcctc caagctgaag
      361 aaaaagaagg gaaatcaaat agagaagagg cgagtattaa aaaaacactg gctgaaattg
      421 aacgtttaga aaatcaaatt aatgaaaccc atgcgcaatt cattaaagat aatatagaaa
      481 ataatgaggt ttttaatttt tttaaagata agtcctatat ttttgaagcg gcaatgcaaa
      541 actatgatgt cgctaaagta ttcatgagtt ccagcgaagt aatgtcttta ttatctccta
      601 agcaagctat agctgtgctt ttcaagcaca gggcaaatat aggccaacaa ttttctttat
      661 tcgatgcaga taacccacgc tttatttcta aagataaccc cactattatt gaggggctaa
      721 aaagtgattc ttttgaagcc aaaagattta taaactctag acttggtgcc ttattaagcc
      781 ctttagaatt tgctaaaatc ttattaactc accaagataa tagcagtttt ttcattcaaa
      841 ctttagctcg gcatgagtat gcttttagtg aagaaagttt aaaagatcct caagttaaag
      901 agatctatca gcaactatta atcaaagcta ctgatccagt taacgcaaga gcttacaaaa
      961 aatattttat tcaacatcct gctcatattc agttattatt taatttctta atggccactg
     1021 aaaatgcaaa tgcagcaatt gcatttatga attcttatga aaacatgtca ttattttctg
     1081 tcaagcaagt cacagaattg cttttaaatc atagagcaca tctaaagcta caaaacgtac
     1141 ctctatttac ccctcataat cctcaactta ttgctttaga taatcctatg cttattaacg
     1201 cgttaaaaaa cgatcctggc atagctatcc aatttataaa ttcgagattg ataaatttat
     1261 ttacaccttt gactgctgca aaaattatgc ttgcccataa agataatgat cgtttctatc
     1321 atcaaacgcg gccacgccat gcttcacttt ttacttctga aaacctgaat gatcccagga
     1381 ttaaagaaat ttatgatcaa tattttcata atcgaaatca gagaaaaaat cgattttttg
     1441 cacaacaacc ccaagttaac gaggctgagg agtatttcag atttgcgaat agagatgaaa
     1501 taaaatttaa tccctataaa gtattaggcg ttaacgaaga tgcaagcttg gaggagataa
     1561 aaaaagccta taaaaagtta gcaatagctt ggcatcctga tagagtacaa gcaggtgaaa
     1621 atatagaagc acgaacggaa cgttttaaga aacttggggt agcctataat attttaattg
     1681 atcctgccaa acgacaggag tatgacaata ggcctcaaaa cagattcgga aggcataatc
     1741 ccatgcatta agggcttcgt tattgggtta gcaatagcga ttgcatccaa aaagggtgat
     1801 actattcaac caggtggtgc tcaattagct aaccaactta gctttgctga tcaagtatca
     1861 gcaacaccgt cttatattgt ttctggtgtc tcccgacaaa aaaccatagc gaactatcca
     1921 ataaaattta gtcattcaaa atttatgaat aatatacctt taaatcccaa tgtgattaag
     1981 gtacttaatg cattaaacca tattggaaag aatcatatta ctgaagacat ggtgcagaaa
     2041 agtaaaaaaa tattgtctca aagagacaaa gcccagctta aaaaatatta aatcagttac
     2101 cagactggat gatttacgtt gttttaaaaa tcctaggaaa ataataatgc aagatctatt
     2161 ggaatttagc tctaaagagc gccaacaagc ttttgaagaa gccactgctc gttccacaac
     2221 aattaagaac ccaattacta ttgagaagga tttttgggta tgctggactc cggatcaaat
     2281 tttctctaat gaagacctgt ctcctcatgt ggtttttatt tgttaatgct gaacacaaaa
     2341 atcacaaacc gcttcaatta aatctctgta cataattgct taccctgaat acggcaagag
     2401 cgacagctag agttaaacca agttttgtta gtgcaatata tcccagccag tgataaattg
     2461 tcgccggctt aaaagctaac ttcgtatcgt tcaatttatg acagagaaaa aatgtttcta
     2521 aaatatctaa ttgtaattat gtttttaatg actagttcat tactttttgc tcaattgccc
     2581 aaggaaattg tagtggcatg ccatgaagga gagactccta atgacagtgt tagttacatg
     2641 tatcttcctg gtaatttacg gatcttggtg cctatttcaa aaacacttac taatagtagt
     2701 attagctata caccccttcc tggaagcttt gattataaga aaattaaagg tagcgaagaa
     2761 cgttatgaat ttgacgtcaa tatgcgtcac tttagtatag aagataatca atattttgta
     2821 agagacaata gttggataaa attatccgat gcgattaatc attcaaatca ccctattgct
     2881 gagccaggga atataaataa tccaatgatg gtagcaaaga ttacgtttaa taaaaaagac
     2941 tatctttgta tatctggctc tttatgtgta aatggctcat tagctgcagt tggtgaatat
     3001 tatattgttg aaaatgcttt tgattatgac aaaaaattac agttatatta ttattttttt
     3061 gataaagaat ttgttgagat ggtgcgaatc agagataatt ggaatggcta attt
//
LOCUS       DATNSN010000246         3036 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.247, whole genome shotgun sequence.
ACCESSION   DATNSN010000246 DATNSN010000000
VERSION     DATNSN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.247"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1362)
                     /locus_tag="VAH76_003003"
     CDS             complement(<1..1362)
                     /locus_tag="VAH76_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO1387579.1"
                     /translation="MSGIDRLIINSPYKEPEFFHQYVRENRNFILTKGRRPAGYIIAS
                     ENSRAFDDPGQFVEIPLVNQIRQRVKSWRNSGYLGVTGITKRLLEHWYDEERYKSGSS
                     TPPFFFCQLEAIETLIWLCEGPNADKVGISIPNEGNEFQRLCSKMATGTGKTIVMAML
                     IAWQILNKISSPRDPRFSKFIFVVAPGLTVKNRLSVLSPNDKNNFYESFNIVPSTMLD
                     KLRQGKIIIKNWHALNWDTEEKIAKRRSVDKRGAKSDEAYVREVLDNISSTKNIIVIN
                     DEAHHAWCVSSETNRLRDVSKEDIEEATKWVGGLNRINRARGILHCYDFTATPFAPSG
                     RRSNSEALFGWIVSDFGLNDAIEAGLVKTPRIVIRDDALPDTKTFQSRLYHIYSDPEV
                     KDDINRSTEPHYPLPSLITNAYYLLGYDWRETLIAWQTAGHKVPPVMITITNSTQTAS
                     RITY"
     gene            complement(1368..>3036)
                     /locus_tag="VAH76_003004"
     CDS             complement(1368..>3036)
                     /locus_tag="VAH76_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEO1387580.1"
                     /translation="AKEKNSLKFNRLYIDRQLNDDSDFTYIELPSGETHTVADYIKKH
                     GTIPNEAKYFQSMDMRSSGRTESCVFEYQINGQKFTPSGGKSWKTNLDGMKQLEKLNR
                     LFAPGNSLRYKFFFEDYPVTELSHMWMDTQGATNMKYVVQTPEKVIQRCILMTTDPGD
                     IVLDPTCGGGTTAYVAENWGRRWITCDTSRVAITLTKQRLMTSIFEYYKLAHPDEGVD
                     SGFLYKTAPHITIGSLTNNEQSLPITLYDQPLLDLSKVRVTGPFTVEAVPAPVVKPLD
                     VLTQMAVNDNSVSRSGESLRQNEWKDELLNTGIRGKNGQRIEFTYIETLGGTRYLHLE
                     AETKEEIPQRVAISFGSNYAPLEQRQVALAIEEALDLVPKPKIIIFAAFQFDPEAAKD
                     IDQTNWPGVVLLKVQMNADLLTEDLKKKRMSNESFWLIGQPDIQLLKCNDGLWQVEVH
                     GFDYYNTKTGNLESGGCDKIAMWMLDLDYDGRSLFPAQVFFPIIGNQEGWSKLAKNLK
                     AEIDNEKIEIYSGTRSLPFEAGEHQRIAIKIVDDRGIESLKIMELPR"
BASE COUNT          880 a          605 c          531 g         1020 t
ORIGIN      
        1 atacgtgata cgtgaagctg tttgggtact atttgtaatg gtgatcatta cagggggaac
       61 tttatgccct gctgtttgcc aagcaatcaa tgtttcacgc cagtcatatc ccaataggta
      121 ataggcatta gtaattaaac tgggaagagg gtagtgtggt tcagtacttc gattgatatc
      181 atctttgact tctggatcag aataaatatg atataaacgg gattgaaatg ttttagtatc
      241 tggcaacgca tcatcccgta taacaatacg tggtgttttt actaagccgg cctcaatagc
      301 atcattcaag ccaaaatcac ttacaatcca gccaaacaat gcttcactat tactccgtct
      361 acccgaagga gcgaaaggtg tagcagtaaa atcatagcaa tgtaatattc ctcttgcgcg
      421 attaatgcga ttcaatccac ctacccattt agtggcctct tctatatctt ctttagaaac
      481 gtctctaagt ctattagttt cagaagatac acaccaagcg tggtgtgcct catcattaat
      541 tacaataata ttttttgtgc tacttatatt atctagcacc tcacgtacat atgcttcatc
      601 acttttagct cctcttttat caacagatcg tcttttggct attttttctt cagtatccca
      661 gtttaatgca tgccaatttt ttataataat cttaccttgt cgtagtttat ctaacatggt
      721 acttggcaca atattaaagc tttcataaaa gttattctta tcgtttggtg aaagaacgct
      781 taagcggttt ttaactgtta atccaggggc gactacaaag ataaattttg aaaaacgtgg
      841 atcgcgggga gaggaaattt tatttaaaat ttgccaagca ataagcattg ccattacgat
      901 tgtcttacca gtccctgtag ccatttttga gcaaagtcgt tggaactcat tcccctcatt
      961 aggaatagaa atacctactt tatcagcgtt aggtccttca catagccaaa ttaaagtttc
     1021 aattgcttct agttggcaaa agaaaaaagg aggtgtgcta cttcctgact tatagcgttc
     1081 ctcatcgtac caatgctcca gaagtcgctt tgtaatacca gttactccaa gatatccact
     1141 gttacgccac gatttaacac gttgtcgaat ctgattaaca agtggtattt caacaaactg
     1201 tccagggtca tcaaaggctc tcgaattttc tgaggcaatg atgtatcctg ctggacgtct
     1261 gccttttgtt aaaataaagt ttcggttttc tcgtacatac tggtgaaaga attcaggctc
     1321 tttatatgga gaattaataa taagtcgatc aatgccagaa atatttttca tcgtggtaat
     1381 tccatgatct ttaaactctc tatgcctcga tcgtcaacta ttttaatggc tatacgctga
     1441 tgttctcctg cttcaaacgg taatgatcga gtccctgaat atatttcaat tttttcatta
     1501 tcaatttctg ctttgagatt tttagcaagt ttactccaac cctcttggtt acctataatt
     1561 ggaaaaaata cttgagcggg gaataggctt ctgccatcgt aatctaaatc taacatccac
     1621 atagcaattt tatcacatcc acctgattct aaattgcctg ttttggtgtt ataataatca
     1681 aatccatgta cttcaacctg ccataagcca tcattgcatt ttaaaagctg aatatcgggt
     1741 tggccaataa gccagaagct ctcattactc attcttttct ttttcaagtc ctcagttaat
     1801 aaatcggcat tcatctgaac tttaagcaaa actacgccag gccaattagt ttgatcaata
     1861 tctttggctg cctcgggatc aaactgaaat gctgcgaata taataatttt gggtttaggg
     1921 actaaatcta aagcttcttc tattgccaat gctacttggc gttgctcaag aggagcataa
     1981 ttagatccaa aagaaatggc tacacgttgt ggaatttctt ctttggtttc cgcttcgaga
     2041 tgaaggtatc ttgttccacc taaagtttca atatatgtaa actcaatgcg ttgcccattt
     2101 tttccacgaa ttcctgtatt cagtaattca tctttccatt cattttggcg aagagactct
     2161 ccggatctag aaactgagtt atcatttact gccatttgtg ttaatacatc caaaggctta
     2221 actacgggtg ctggcactgc ctcaactgtg aatggtcctg ttacgcgcac tttagaaagg
     2281 tctagcaaag gctgatcata taatgttata ggcaaagatt gctcattgtt agttagtgac
     2341 ccgatggtta tatgaggagc ggttttataa aggaagccac tatctacgcc ttcatcagga
     2401 tgagctaatt tataatattc aaaaattgaa gtcattaatc tttgtttagt taatgttatt
     2461 gctacacgag aagtatcaca tgttatccag cgacgccccc agttttcggc aacatatgca
     2521 gtggttccac caccacatgt agggtcaagt actatatctc cagggtctgt tgtcattaaa
     2581 atacatcgct gtataacttt ttcaggggtc tgtactacat atttcatgtt tgtagcacct
     2641 tgcgtatcca tccacatatg acttaattca gttactggat aatcttcaaa aaagaattta
     2701 taacgaagtg agttccctgg agcaaaaagc cgatttagct tttcaagctg tttcattcca
     2761 tcaagattgg ttttccaact ttttcctcca gatggagtaa atttttgacc atttatctga
     2821 tactcaaata cacaagactc agttctacct gaggaacgca tatccataga ttggaaatat
     2881 tttgcttcat ttggaatagt gccatgcttt ttaatgtaat cagcaacagt atgagtctca
     2941 ccagagggta attcaatata tgtaaaatcg gaatcatcat ttagctggcg atctatatat
     3001 aatctattga attttaaact attcttttct tttgca
//
LOCUS       DATNSN010000247         3016 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.248, whole genome shotgun sequence.
ACCESSION   DATNSN010000247 DATNSN010000000
VERSION     DATNSN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.248"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..942
                     /locus_tag="VAH76_003005"
     CDS             <1..942
                     /locus_tag="VAH76_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HEO1387581.1"
                     /translation="IRRHLDTIAANRPDVKNLLFILVATPVEEVGRDHDFDWAVIEPS
                     SYRSIIQLAGRVMRHREDTIEYPNIAILQYNWRTIKYGDAERKPRFYFPGYEPDPRKR
                     QTNKLSNGKNDCLNSHDIFKIVDINLIKDRLDSIPRIQERPENSKTPMALLEHSVIES
                     LLTYYEGKGPETLQGYIQGYWRLTALPQYLNRFRQSTKTTQLYRVANETGEAWFTERD
                     TRGYFSYVNTGEFARQDDAYQISTLPLTAEQSQRLWIQRDYLHLLREQMEISEMSIDK
                     AAIHYGEISLDVYPDGINTEYEYNDQLGLYKKESNHV"
     gene            935..2200
                     /gene="csy1"
                     /locus_tag="VAH76_003006"
     CDS             935..2200
                     /gene="csy1"
                     /locus_tag="VAH76_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HEO1387582.1"
                     /translation="MFDPAITDFFAERKKSWLKKKLKSDMTELEKNNILQECEDIFAL
                     ENWLPNAAKRAGWMSISTHPCTFSHPSSRKNKNGYVTSIIAKAPRKADGYLRSGNVHV
                     ALDALGNAAALDVYKFLSLVTEDGKTIIEHIQQNTEVAKSLLSIKTDSYENLRVGFLE
                     MVSSNEASITSSKIKQVYFPMGNEYHQLSVLSNSGMIFELRERIDALRFSDTVQRERE
                     FRRDNIYSELGYKEIYDVTTIAYGGTKPQNVSVLNNQYGGKAYLLFSAPPMLEQREIT
                     FPTKDFFVQSIRYYDCKEILEQLDNIFKIERYGKISLDKIRKGRDRCLSDMLDMILQK
                     MMVLREASKNQFRSQSSSLPAWQKIWLCEQYIEERGKRDDWLETLCKQIALWINSAYK
                     NSIKHPTMLGEAERRYFKDFIDEHREVLR"
     gene            2197..>3016
                     /gene="csy2"
                     /locus_tag="VAH76_003007"
     CDS             2197..>3016
                     /gene="csy2"
                     /locus_tag="VAH76_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HEO1387583.1"
                     /translation="MRSNLILLPSIRVQNANALSSPFTIGFPAMTGWLGGVHALQRKL
                     NTNSIAVMFSAVGVVVHRFDLQTYKGPGDFVHSIIGTGNPLEPKSEKDKPKGNAVRPS
                     FIEEARCHLEVSLLVEYSGIEAQDEQLMLERVYQLMLGNMKLAGGDVLNCKEPEILRD
                     FQKAKRKLMPGYALIERRDLMQAAMEQGQDAMDAMLDYVAVHYSCTKEIVNGQERVIW
                     QRKRKSTGPDGQTGWIVPIATGFHGISELGTAINQRDPDTPHRFAESLVTLGEFK"
BASE COUNT          938 a          573 c          675 g          830 t
ORIGIN      
        1 attcgcaggc atcttgatac cattgctgct aaccggcctg atgttaagaa cttgctattt
       61 attctggtag ccacacccgt tgaagaagtt ggccgcgacc atgattttga ttgggctgtt
      121 attgagcctt cttcatatcg atccattatt caattggctg gacgggtcat gcgacatcgt
      181 gaggatacga ttgaatatcc caatatcgct attcttcaat ataactggag aacaattaaa
      241 tacggggatg cggaaagaaa acctcgcttc tattttccag gttatgagcc agatccacga
      301 aagcgccaaa caaataaatt aagtaatgga aaaaacgatt gtttgaacag tcacgatatc
      361 ttcaagatag tcgatattaa tttaataaaa gaccgacttg attccattcc acgaattcag
      421 gaacggccag aaaatagcaa aacccctatg gctttactgg agcattcagt aatagaaagt
      481 ttactcactt attacgaagg aaaaggcccg gaaaccttgc aaggttatat tcaagggtac
      541 tggcgtttaa cagctttgcc acaatatctt aatcgctttc gccaaagcac aaaaaccaca
      601 cagctatacc gggtagccaa tgaaacaggt gaggcctggt ttactgaacg tgatacaaga
      661 ggatatttta gttatgtcaa taccggtgaa tttgcccgcc aggacgatgc ttatcaaatc
      721 tcaactctgc cgttaacagc agaacagtct caacgacttt ggattcagcg tgattattta
      781 catttgttgc gggagcaaat ggagatatcc gaaatgtcta tagataaggc tgcgattcac
      841 tatggtgaaa tcagtttgga tgtttatcct gatggcatca acacagaata tgaatacaac
      901 gatcaattag gtttatataa aaaggagagc aaccatgttt gatccagcaa ttactgattt
      961 tttcgctgag cgaaagaaaa gctggcttaa aaagaaactg aaatcggata tgactgaact
     1021 tgagaaaaat aacattctac aggaatgtga agatatattc gcacttgaaa actggctgcc
     1081 gaatgcggcc aaacgtgcag gatggatgtc tatttcgaca catccttgta ctttctccca
     1141 tcccagttcc agaaaaaata aaaatggcta tgtaacatcc attattgcta aagcaccccg
     1201 taaggcagat ggctatctcc gatcaggcaa tgttcatgtt gcattagatg ctcttggtaa
     1261 tgctgcagcg ttagacgttt acaagtttct atctcttgtt actgaagatg gaaaaactat
     1321 tatagagcat attcaacaga atacagaggt agcgaaatcc ctacttagta taaaaacaga
     1381 tagttatgaa aatttaagag tcggattttt ggaaatggtt tcttctaatg aagccagcat
     1441 tacaagttct aaaattaaac aagtctattt tccaatgggt aacgaatacc accaattatc
     1501 tgtcttaagt aattcaggca tgatttttga gttgcgtgag aggattgatg cattgcggtt
     1561 ttcagataca gtccaaagag agcgcgaatt ccggcgtgat aatatctatt ctgagttggg
     1621 ttacaaggaa atctatgatg taactacgat cgcgtatggt ggcacaaaac ctcagaatgt
     1681 cagtgtgctt aataatcaat acggtggaaa ggcatattta ttattctccg ctccgccgat
     1741 gcttgagcaa cgagaaatta catttcctac taaggacttt tttgtccaaa gcatacgtta
     1801 ctacgattgt aaggaaattc ttgagcaact cgataatatt ttcaaaatcg aacgttatgg
     1861 gaaaatatcg ttagataaaa ttcgcaaagg aagggatcgt tgtcttagtg atatgctgga
     1921 tatgatcttg cagaaaatga tggtcttacg tgaggcatcc aaaaatcaat ttagatccca
     1981 atccagtagc cttccagcct ggcaaaaaat atggctgtgt gagcaatata tagaggaaag
     2041 gggcaaacgc gatgattggc tcgaaacgct gtgcaagcaa attgcacttt ggataaacag
     2101 tgcatataaa aacagcatca aacatcccac tatgttgggt gaggctgaac gtcgttattt
     2161 caaagacttt atcgatgaac atcgggaggt attgagatga gaagtaacct gattttattg
     2221 cccagtattc gagttcaaaa cgccaatgca ttatccagcc ctttcaccat tggttttcca
     2281 gctatgacgg gttggctagg tggtgtacat gctttgcagc gcaaattgaa tacaaacagt
     2341 atcgctgtga tgttttctgc ggttggtgtt gtggtgcatc gctttgattt gcaaacctac
     2401 aaagggcctg gtgattttgt gcactccatt attggaaccg gaaacccctt ggagccaaaa
     2461 tctgaaaaag acaagcccaa aggtaacgct gttcgaccat catttattga agaagcccgc
     2521 tgccatctgg aagtcagcct tcttgttgag tactcaggca ttgaagcgca ggatgaacag
     2581 ttgatgttgg aacgagtata tcaattaatg ctcggtaaca tgaaactggc tggtggagat
     2641 gttctcaact gcaaggaacc tgaaatcctt cgggattttc agaaggcaaa gcgaaaacta
     2701 atgccgggtt atgccttaat tgaacgaagg gatttaatgc aagcagccat ggagcaaggc
     2761 caggatgcta tggacgcaat gctggattat gtggctgtgc attacagctg caccaaagaa
     2821 attgtgaatg ggcaggagcg tgtgatctgg caacgcaagc gaaaatcaac ggggcctgat
     2881 ggccaaacag gctggattgt tcctattgct acagggtttc atgggattag tgaattagga
     2941 acagcgatta atcagcgtga tccagatact cctcatcgct ttgctgaaag ccttgtgacg
     3001 cttggtgagt ttaaaa
//
LOCUS       DATNSN010000248         3009 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.249, whole genome shotgun sequence.
ACCESSION   DATNSN010000248 DATNSN010000000
VERSION     DATNSN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.249"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1257)
                     /gene="lem17"
                     /locus_tag="VAH76_003008"
     CDS             complement(<1..1257)
                     /gene="lem17"
                     /locus_tag="VAH76_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem17"
                     /protein_id="HEO1387584.1"
                     /translation="MEDESKVKKGLKEEKEQLNQKWLEQLLNNPRWGPSVVKLAMARK
                     KENRNKRGEAFNCFVLSRPQNAFEEDEFRKFLDKLKNNINDFSGTTRFQIALFTADNH
                     HWTAFDFFIVDGKLHVFCLDAANDSAGDAALDEIMSRFPGCDAYRLEPDRDPKAKDSK
                     HFRLIQTDRENCSRMTIEHILLLSKKKFLFNENFANYKVVNGVKLINPDALFRQSPEL
                     YRMTQIDSILDSLMIASPKEAQRLVSKKGLNAEQYRDLLGHTSGKQSKSSKNTTIDYK
                     RQRVEKNLLAFLAESEQKHLEDILDNSEKQWSEFLEMSQRERIQFLLGNIRERPFKEI
                     VGNILGKGIKDRLDHKKYIENDVIKKSSFTLFRGIDKHFEIKKIKAEINELEKALKIV
                     NKLSNDTSADEVLLHLQKQKLPLGIKT"
     gene            1491..2687
                     /gene="ralF"
                     /locus_tag="VAH76_003009"
     CDS             1491..2687
                     /gene="ralF"
                     /locus_tag="VAH76_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214205.1"
                     /note="RalF, a virulence factor secreted by type IV
                     secretion systems (T4SS), is a guanine nucleotide exchange
                     factor that acts on host cell ADP-ribosylation factors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS guanine nucleotide exchange effector RalF"
                     /protein_id="HEO1387585.1"
                     /translation="MHPEIEKAQREIIEAFNAKPKNGIKKIKEICGEYKISPNEEIAA
                     FFHQQRKNLDLEAVGDYLSEPEAENQQVLKAFTSQMDFNGQSFVESLRIFLKTFKLPG
                     EAQKIDRLVQSFSGAYSQQNPGVVSNADAAYLLAFQTIMLNTDLHNPSIPEKNKMTMD
                     GLKRNLRGGNNGEDFDAKFLEELYSEIKAKPFELNFVKTSPGYELTSITLNKDSTFKK
                     LDSFLHSTDVNINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDKQTSAQATIQ
                     VYTPNIFSKWLFGEQPRVIIQPGQTKESIDLAAKIAAGFSSPVKNFKATYDYEAGDLI
                     KAYDNQKKLISTNKNTVIKGAMFRTPNAEQQETSKSATKTLNTDYDINEDTETIKDES
                     KSSYKK"
BASE COUNT         1009 a          556 c          509 g          935 t
ORIGIN      
        1 tgtttttatc cccaggggta atttttgttt ttgtaaatgt aacaatactt catcagcgct
       61 tgtgtcatta gataatttat ttactatttt caaggctttt tccagttcat taatttctgc
      121 tttaattttc ttgatttcaa aatgcttatc aatccctcta aaaagagtaa aggaactttt
      181 tttgatcacg tcgttttcaa tgtatttttt atgatccagc ctgtctttta tgcctttgcc
      241 tagaatattc ccgacaattt ctttaaaagg tctctctctg atattgccca ataaaaattg
      301 tatgcgttct ctctgagaca tttctaaaaa ttcagaccat tgtttttctg agttatcaag
      361 aatatcttcc agatgcttct gctcactctc ggccaaaaaa gctaaaaggt ttttctccac
      421 cctctgtctc ttgtaatcga tggttgtatt ttttgatgat ttggattgtt tacctgatgt
      481 atgtccaagc aaatccctgt attgctctgc atttaaacct ttcttactga ctagtcgctg
      541 cgcctctttt ggtgaagcaa tcataaggct atccagaata gaatcgattt gagtcatgcg
      601 atacagctct ggagattgcc tgaatagtgc gtcaggatta atcagcttca ccccattaac
      661 cactttgtag ttggcaaaat tttcattaaa taaaaatttc ttttttgaca acagcaaaat
      721 atgctcaatg gtcattcgtg aacaattttc tcggtccgtt tgaatcaaac gaaaatgttt
      781 tgaatccttc gcctttggat ctctatcagg ctctaaccgg taagcatcgc aaccagggaa
      841 tcggctcatg atttcatcaa gcgcggcatc tcccgcacta tcattagccg catccaaaca
      901 aaaaacatga agcttcccat caacgataaa aaaatcaaat gctgtccagt ggtggttatc
      961 tgcagtaaaa agagcgattt gaaatcgggt cgttccacta aaatcgttaa tgttattttt
     1021 taacttatcc aggaattttc ggaactcatc ttcctcaaac gcattttgtg gtctgctcag
     1081 cacaaaacag ttaaatgcct caccacgttt attccgattt tctttttttc ttgccatagc
     1141 taacttaacc acggaagggc cccagcgcgg gttattcaat aactgctcaa gccacttttg
     1201 attaagttgt tctttttctt cttttaaccc ttttttgact ttggattcat cttccatagc
     1261 cgcttatctt caatctgatt ctatattatc tagtatagaa cgtataaagc acactacgat
     1321 atttttgtaa tcatcaagaa cttaagtctg caaatgtttc ttgtctaaaa aggcaaaatt
     1381 agatcaattg gattataata taaccacaaa cgatcgctat taaaacggaa tgatgattaa
     1441 ttttttatct aagcaaaatt catcagttca ttcgacaaaa ggagcagctt atgcatccag
     1501 aaattgaaaa ggcacaaaga gagattattg aggcttttaa cgccaaacct aaaaatggga
     1561 taaaaaaaat aaaagaaata tgtggggaat ataaaatttc cccgaatgaa gagattgctg
     1621 ctttctttca tcaacaaaga aaaaatttag atttagaagc ggttggcgat tatttgagtg
     1681 agccagaagc agaaaatcag caagtattaa aagcttttac cagtcaaatg gatttcaatg
     1741 ggcagagttt tgttgagagt ttaaggatct ttttgaaaac atttaaatta ccaggagaag
     1801 ctcaaaaaat tgacaggtta gtgcaaagct ttagtggcgc ctattcccag caaaatcctg
     1861 gtgtggtttc caatgcggat gctgcctatc ttttagcatt tcaaaccatt atgcttaata
     1921 ctgatttgca taacccaagt atccctgaga aaaataaaat gactatggat ggactcaaaa
     1981 gaaatctcag aggaggtaat aatggtgaag attttgatgc caaattttta gaggaactat
     2041 acagtgagat taaagccaaa ccttttgagt tgaatttcgt taaaaccagc ccaggatatg
     2101 aacttacttc aatcactctc aataaagatt ctacctttaa aaagctggat tcattcttgc
     2161 attcaacgga tgtaaacatc aataccgttt ttccaggaat aggtgataat gttaaaacaa
     2221 ctgtagatca accaaaatca tggttaagtt tttttaccgg atataaggga acaataacgt
     2281 taacggataa gcaaacatcc gcacaagcta ctatccaggt ttacactccc aatatatttt
     2341 caaaatggct atttggagaa caaccgagag taataataca acctggacag accaaagagt
     2401 caatcgattt ggcagcaaaa atagcagctg gtttttcatc gccagtaaaa aattttaaag
     2461 cgacgtatga ttatgaggca ggtgatttaa taaaagcata tgacaaccag aaaaaactca
     2521 tttcgaccaa taaaaatacg gtaattaagg gggctatgtt cagaacacca aacgctgaac
     2581 aacaagaaac ttcaaaatct gcaacaaaaa cgcttaatac tgactatgat ataaacgagg
     2641 atacagagac tataaaagat gaaagtaaat cgagttataa gaaataataa tttataaaag
     2701 attagcttaa tgtcaacttg attttcatcc tgatttttat cactgagaag attgaaatgc
     2761 acgcactaca agcgcagcaa acatgaagac aaagtaaacg ttttaagagt tccggccacc
     2821 aacttagtag cagtttatgc agtattagtt aacaagggct tgatgggata tatagataat
     2881 atgtaatagt tcaaacctaa tctgacacca ttgctagaaa aagtgagagc tcccgttttt
     2941 gatgaaagtg caaaccttaa acaaaaccaa gaaaaactga tagtacccta aaacaagttt
     3001 acctatgaa
//
LOCUS       DATNSN010000249         2995 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.250, whole genome shotgun sequence.
ACCESSION   DATNSN010000249 DATNSN010000000
VERSION     DATNSN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.250"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1236
                     /gene="rvfA"
                     /locus_tag="VAH76_003010"
     CDS             <1..1236
                     /gene="rvfA"
                     /locus_tag="VAH76_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RvfA"
                     /protein_id="HEO1387586.1"
                     /translation="ITIHTWKGGTKGLGFKTPVKSTLFGGNVGHAALELTWPANKQGD
                     ELAKKYAQAHGVIISKRTEIVGETQEEGRIMPKERVTYFAYFSWWPGEQNGHYINSFN
                     DDKDAEWRHEPDKKMHADWKNQIYGPQGMSEENTTDVKGILIKNKNIQKLKTIQHSTL
                     TADIAQDKAYNELLQHKEKLDEEYKSLHAKAVAYQKEKENAIREEREINPNFELLDED
                     ISRMGDLPREFSILEAQLNLCIADFNEKHLSSGKKPDSSVILPTSMDETSLNHTLDTE
                     SILREMITLANSEKAYNFTKFNCSTSVCHIVKAGVDEKLKENLSDNGFNINRYVTSYI
                     TTPTNVNALGLKLQDALLKLELAEHTKEAEQVQKPVANTISNRFNEFKSRLQAVVSER
                     NAHKDDTEEVTINNHNSLR"
     gene            complement(1421..2617)
                     /gene="mavB"
                     /locus_tag="VAH76_003011"
     CDS             complement(1421..2617)
                     /gene="mavB"
                     /locus_tag="VAH76_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavB"
                     /protein_id="HEO1387587.1"
                     /translation="MPPPYSTHVIVNVFRCVAQLVSQNPELLNWPGVFRLAGSREESE
                     KLLEQVINDNFSVGNLACYILEDGQINNLHLNNVLGMLPLVLKESVLLKTEDPLLKLF
                     MTELKNLLSSEKNNVENTDAIVQLLSKFIDSLLLSKILDHQRVGEILYHYCYLMHTAG
                     VFYETNLMTWENLAIIMAPHFTNEFGLYPAEDLLGLIQFTNQLKPILECFIAHPDSGI
                     PFKERHADKLEHLAKTRHTIVEKLTHMGSESRRIVVAPMKSLMLQASMLRAQIDTVET
                     QSKDSSLKKKDKKELNRQLEKLTEELEKLNIEISGLTSKIKKMNHGHAKLKEEIRVIS
                     RSEDAVSTHAAQQSGVSSSSSNSVRIQLGIFGNNSSSSAYLIEEQEEGEVTENEYSGL
                     DSAKLT"
BASE COUNT         1051 a          530 c          499 g          915 t
ORIGIN      
        1 attactattc atacttggaa aggaggtact aaaggattag gctttaaaac ccctgtaaaa
       61 tctactttat ttggtggtaa tgtgggacac gctgctttag aattaacttg gccagctaat
      121 aaacaaggag atgaattagc aaagaaatac gcacaagccc atggcgtaat aattagtaaa
      181 agaactgaaa ttgtaggaga aactcaagaa gaaggtagga tcatgccaaa agagcgagta
      241 acctactttg cctatttcag ctggtggcca ggagagcaaa atggccatta tattaattct
      301 ttcaacgacg ataaggacgc tgaatggaga cacgagcccg ataaaaaaat gcatgctgat
      361 tggaaaaatc aaatctatgg cccacaggga atgagtgaag aaaatacaac agacgttaag
      421 ggaatactga ttaaaaataa aaacatacaa aaattaaaaa ctattcagca cagtactctg
      481 acagctgata tagctcagga taaagcttat aatgagcttc tacaacataa agaaaaatta
      541 gacgaagaat ataaatcatt acatgcgaaa gcagtcgcat atcaaaaaga aaaggaaaat
      601 gctatacgag aagaaagaga aataaatcct aattttgaac tgcttgacga agatatttct
      661 agaatgggag atcttccaag agagttctca attttagaag cgcaattgaa tctatgcatc
      721 gcagatttta atgaaaaaca tctgagttca ggaaaaaaac ctgattcaag cgtgatttta
      781 cctacctcta tggatgaaac cagtttaaat cataccttag atacggaaag cattttacga
      841 gaaatgatca ctctagcgaa cagtgaaaaa gcttacaatt ttaccaaatt taattgctct
      901 acctcggttt gccatattgt gaaagcagga gtggatgaaa aattaaagga aaatttaagc
      961 gataatgggt ttaatataaa tcgttatgtg acttcctata tcaccactcc aaccaacgta
     1021 aatgctttag gattaaaatt acaagatgcg ctgcttaagc ttgaactagc agaacataca
     1081 aaggaagcag aacaggtaca aaagccagtg gctaatacga taagtaatcg ttttaatgaa
     1141 tttaagtcaa gactgcaagc cgtagtatcg gagcggaatg cgcataaaga tgacaccgaa
     1201 gaagtaacga ttaataatca taattccctg aggtagtgtt taaatgtagt agttcaaact
     1261 tgatctgaca gttaccggtt tttcaagaag tgtctatcag gtcaaattta gtttataaat
     1321 ttttccatcc agatttgttt atcctctacc aaagtttgca ttggagtacg accacaacac
     1381 attttaccgc ccaagagatc tcaattaaaa actattgaat ttaagtcaac tttgcactat
     1441 ctaagccact atattcattt tcagtaactt cgccttcttc ctgttcctct atcaaatagg
     1501 cagatgaaga actgttgtta ccaaaaattc ctaactgaat tctgactgag ttactactgc
     1561 ttgatgatac tcctgattgt tgggcagcat gagtacttac agcatcctca gagcgcgata
     1621 tcactctaat ttcttctttt agcttcgcat ggccatggtt cattttttta attttgcttg
     1681 ttaatccaga aatttctata ttcagttttt ctaattcttc tgttaatttt tctaactgtc
     1741 tatttaattc ctttttatcc ttctttttaa gagaggaatc cttcgattga gtttctacag
     1801 tatcaatctg ggctctcaac atagatgctt gcagcatcaa acttttcata ggagccacaa
     1861 ctattcttct ggattcactt cccatatgcg ttaatttttc aacgatagta tgacgagttt
     1921 tagccaagtg ctctaattta tcggcatgcc tttctttaaa agggattccg ctatcaggat
     1981 gcgcaataaa acattccagg atgggtttta actgattagt aaattgtatt aaacccagta
     2041 aatcttctgc agggtataaa ccaaattcat tagtaaaatg aggggccata ataatagcaa
     2101 gattttccca tgtcatgaga ttagtttcat aaaaaacccc tgctgtatgc attaggtaac
     2161 aatagtggta aagaatttct ccaacacgtt gatgatcaag tatttttgaa agcaataaag
     2221 agtcaataaa cttactcagc aactgtacta ttgcgtcggt attttcaaca ttattcttct
     2281 cagaactcaa tagatttttt aattctgtca taaaaagctt gagcaatggg tcttcagttt
     2341 tcaacaagac actttcttta agaactaaag gcaacattcc taaaacatta ttcagatgta
     2401 aattatttat ctggccatct tctaaaatat aacacgctaa attccccaca ctaaaattgt
     2461 cattaataac ttgctcaagt aacttttctg actcttctct agatccagcc aatcgaaata
     2521 ctcctggcca gtttaatagc tctggatttt gtgacactaa ttgggcaaca catcgaaata
     2581 cattgacaat cacatgagta ctatatggtg gtggcataac aaattcccag attaaatttt
     2641 gaacaaaata tcatgggatc aaaactaaat caattattgt taatgaaata cgaacctgaa
     2701 tttttccagc cacattaatg tcttttggat taattgaatt catctcaagt gttacttgcg
     2761 atgaaacttg tgttccagaa taaaatcatt attgtctaat caggaacggc ctattctatt
     2821 aggccgttcc tgattaattt gaggaagcaa aaatcacttt aaaattttaa tcattggcaa
     2881 tattgttttt attaccccaa aaaattgcaa aatataaatg attaaaataa ttaataccaa
     2941 tacatttaac agacttttaa tagccccggg cataggtata aaaacattaa ttaac
//
LOCUS       DATNSN010000250         2971 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.251, whole genome shotgun sequence.
ACCESSION   DATNSN010000250 DATNSN010000000
VERSION     DATNSN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.251"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..704
                     /locus_tag="VAH76_003012"
     CDS             <1..704
                     /locus_tag="VAH76_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387588.1"
                     /translation="RHQGKNAKGGYINLGINQVIWEHVKNIKNNNKTSIFTSEAILNN
                     RYQKDINPDDINSSIYNINTTTLSKRHEQIPPEWEKINFEPLSHIGFSKTQIKQLIGK
                     NDPTIVQESIYHFAFGLEYNQKTKKYDDPLNVLMGVLRKGQAWIEGDYRSAIEIAQEK
                     LLESKKAEIERKKSLEEKAFKLAFHEWESEITESELQKLTERKNGDLTPPKAKLTMYF
                     RENIWPSKKGEYLLF"
     gene            993..2447
                     /locus_tag="VAH76_003013"
     CDS             993..2447
                     /locus_tag="VAH76_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387589.1"
                     /translation="MDSVKMNRKGVVYLHWLNGISTFSFAILFSSLSLYLTKNIGLSQ
                     VQSNGVVGFFLASNFILHFIAGYLGDKWLSNRLLFATSTIVQSIGLIVLNFSESFVYL
                     GLSLFVIGCGLGSTCINCLITQQFNSQEDELREKAFFHNYGAMNIGFLSGYILSGYID
                     INDKYEHLFEMSNLINLITVFFIIKSWKHFAKKTVAIRKEIGDGKLGLLLILLIIPIL
                     FAGFYYYWLANGLILSIGAAALFYITLLAHGFKNNNERKKIYSFIFLTVSSIVFWMLY
                     FVGPMGVTQFLKYNVNIYVGTYYIPPQWLMNLNSLFVIIGSPLMISLLERLRKKHIII
                     SIPKQFICSLVFIALSFSVLALGILKSSPDGLTSVNWIVAHYFFQAVGELLIAPVGYA
                     MIGHLAPEKLQGLMMGIWMMASGVSVTLSNYFSNLMTQSESLNPFISNEHYMSVFNQL
                     GLYAVLGALILWLFSKTIENSIQKTSNRTIEGIA"
     gene            complement(2449..>2971)
                     /locus_tag="VAH76_003014"
     CDS             complement(2449..>2971)
                     /locus_tag="VAH76_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387590.1"
                     /translation="KGMLLNVQKSHGVKLSSPQKVLDEIVFSLSNKEQFQNIDTFQHK
                     INIISQLLRNNRWRTPKGFNKYSPEAKRYQEQCDQENTARLKIKKDECTYSGLNEIST
                     NKRLHNVYDLNRRACSNINLQNSLQLQQSLVNGIKKDIKTIKNPTILENFLKILAQEE
                     IQLSKIQGELSLQ"
BASE COUNT          974 a          407 c          530 g         1060 t
ORIGIN      
        1 ttagacatca gggaaaaaat gcaaaaggcg gctatattaa cttaggcatt aatcaggtta
       61 tctgggaaca tgttaaaaac ataaaaaata ataataaaac ttcgattttt actagcgaag
      121 ctatattgaa taatagatat caaaaagata tcaatccaga tgatataaat agtagtattt
      181 ataatattaa tactactact ttatctaaaa gacatgaaca aatacctccg gaatgggaaa
      241 aaattaattt tgaaccattg tcacatatag gattttctaa aacacagata aaacaattaa
      301 tcggaaagaa tgatccaacg atagtccagg aatcaattta tcattttgca tttggtttgg
      361 aatataatca aaaaactaag aagtatgatg atcccttgaa tgtattgatg ggagttttaa
      421 gaaaaggaca agcttggata gaaggcgatt atcgttcagc gatagaaata gcacaagaga
      481 aactgttaga aagcaaaaaa gcagaaattg aacgtaagaa gagtcttgag gaaaaggctt
      541 ttaaattagc tttccacgaa tgggaaagtg aaataactga aagtgaatta cagaagttaa
      601 ccgagcgaaa aaatggagac cttacgcctc ctaaggcaaa gttaactatg tactttagag
      661 aaaatatttg gccttcaaaa aaaggggagt atcttttgtt ttaaattcag agagatatgt
      721 taaatttacc tgcaagtaaa ttatcagagc atatctacat tttccgttaa atattgctat
      781 acctgctggg aaaatggatt tagctatatg tgcaaaaata tattgagttg gttctataaa
      841 tttatcgcta ttcttaaaca ttctttatta ttggttccaa taatacactt aaaaaaatta
      901 gtgatgtaaa atttattagc ttttgattgt aggttttatt cgaaaataat ttgcattatg
      961 caagttatta ctacctgagg ataggatatt aaatggattc tgtaaaaatg aataggaaag
     1021 gggtagttta tttacactgg ttaaacggaa taagtacatt cagttttgct atcttatttt
     1081 catcattatc actctacttg actaaaaata ttggtttaag tcaagtgcaa tctaatgggg
     1141 ttgtaggttt ttttctggct tcaaatttca ttcttcattt tatagcaggt tatctcggtg
     1201 ataagtggtt aagtaatcga ttgctatttg ccacttcgac aattgtacaa agtattggcc
     1261 tcatagtgct gaatttttcc gaatcatttg tatatttagg attaagttta tttgttattg
     1321 gatgtggttt aggctccacc tgcattaatt gccttattac acaacaattt aatagtcaag
     1381 aggatgaatt aagagaaaaa gccttttttc ataattacgg cgcaatgaat atagggtttc
     1441 tttccggcta tatcttaagt ggatacattg acatcaatga taaatatgaa catttatttg
     1501 aaatgagtaa tctgattaac ctgattacag tatttttcat tattaaatca tggaagcatt
     1561 ttgctaaaaa aacagtagct atcagaaaag agatcgggga tggtaaattg ggattattat
     1621 taattttatt aataatacct attctctttg cgggatttta ttactattgg ttggcaaatg
     1681 gactcatact ttctatagga gctgcggctt tattttacat aacgctattg gcacatggtt
     1741 ttaaaaataa taatgagaga aaaaaaattt attcttttat atttttgact gtaagttcta
     1801 ttgtattttg gatgctttac tttgtcgggc cgatgggagt tactcaattt ttgaagtata
     1861 acgtgaatat atacgtaggc acatattata ttccccctca gtggttgatg aatttaaatt
     1921 cactttttgt gattattggt tcacctttaa tgatctcttt attagagcga ctaagaaaga
     1981 aacacataat tatttctatc ccgaaacaat ttatttgttc cctagtgttt atcgcattgt
     2041 ccttttcagt gttagctctt ggcatattaa aatcaagccc tgatgggtta actagtgtga
     2101 attggattgt tgcacattat ttttttcaag cggtaggaga gttgctcatt gctccggtgg
     2161 gttatgctat gataggacat ctcgcgccgg agaaactgca ggggcttatg atgggaatat
     2221 ggatgatggc ttctggtgta tcggtcacat tgtccaatta tttttccaat ttaatgactc
     2281 aatccgagtc attaaatcct ttcattagca atgagcacta catgagcgta tttaatcaac
     2341 taggtcttta tgctgttctt ggagcgttga tactgtggtt attttctaag actattgaaa
     2401 acagtataca aaaaacgtca aatcgaacaa ttgaaggaat cgcataatct attggagtga
     2461 taattccccc tgtattttgg atagttgtat ttcttcttga gctaatattt ttaagaaatt
     2521 ttccaatata gtaggatttt ttatcgtttt aatatctttt ttaattccat taacaagact
     2581 ttgttgcagt tgaagtgaat tttgtaagtt aatattagaa caagctcgac gattcaaatc
     2641 atacacatta tgtagacgtt tatttgttga aatttcattt aaccctgaat aagtacattc
     2701 gtcctttttg attttcaatc gagcagtatt ttcctgatca cattgttctt gataacgttt
     2761 agcttcaggt gagtatttgt taaatccttt cggtgttctc cagcggttgt tacgaaggag
     2821 ttgagaaatg atattaattt tatgttgaaa agtgtcaata ttttgaaatt gctctttatt
     2881 gcttaatgaa aatacaattt cgtctaaaac cttttgaggg gatgatagct ttactccatg
     2941 gcttttttga acattcaata acatcccctt a
//
LOCUS       DATNSN010000251         2960 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.252, whole genome shotgun sequence.
ACCESSION   DATNSN010000251 DATNSN010000000
VERSION     DATNSN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.252"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..855
                     /locus_tag="VAH76_003015"
     CDS             <1..855
                     /locus_tag="VAH76_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO1387591.1"
                     /translation="ALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQC
                     CELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSPISI
                     RYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLL
                     WIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGA
                     WLGWMYLPLILLISSITGAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLII
                     NWYIGYWM"
     gene            complement(869..2074)
                     /locus_tag="VAH76_003016"
     CDS             complement(869..2074)
                     /locus_tag="VAH76_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO1387592.1"
                     /translation="MNQNNASKKDNDNSNQLDTELIGSYQSITIGFSKDELASFSKIG
                     QHKHYTAGSIVYNENDLADSFFIIIDGVAEIFKKTIDEFDEHNIHILATLSKDDVVGE
                     MALIENSRRSASVKAKTDLSVIEYPLEVVKAKPKLNLMLIKNMAQILSRRLRFTNEVT
                     VKNMQQALKEASGRNTLGVFIIALLWIICLYTLSLHFLVTVIHQVTVTTVLSICLISI
                     FAVAILIAMHFTGLPYSRFGITAKDWGKTILEALAFTIPIMLFVLIIKVFFIIGIDHL
                     DNTPIFSGTKRFLVNNEVDWEYYFAMIFLYSLFAPIQELMARSALQSTFFHFLPGGKL
                     FRQWNGIFLSNLIFATMHTHLGLTFASLTFIAGLFWGWLFHRQKSLLGVSVSHVILGV
                     WSLFIVGLR"
     gene            complement(2182..2775)
                     /locus_tag="VAH76_003017"
     CDS             complement(2182..2775)
                     /locus_tag="VAH76_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387593.1"
                     /translation="MFIFKRWKIRKITKRIKAMQANRISNQPGDEVLKKEILYYFELA
                     TIFKKLKNHKKYPYAEIMMIECYRAAANLDDSAANFQLGQIFLDEAKYRQKLDNEGIF
                     NSQANLKRAQQLFDEAHAHLIAAEKLGHVGAKRLRGLCIINGWGVESDKNAGFELVVD
                     SIEQEGSWDKIPQIFASMGLNKPEFFSAIMQRRKGTS"
BASE COUNT          908 a          528 c          503 g         1021 t
ORIGIN      
        1 gcactaataa tcaattatcc atggtttatg tatttagtcg ttggactctt ttctctagct
       61 gttggaagtt tgcttaatgt gataatctat cgcttaccaa ttattttgca ggaagagtgg
      121 aaagagcaat gctgtgagct attccagctt gaacagcgaa aagaaaaaat aaaacttaat
      181 ttatttttac cacgttcttt ttgccctcat tgtaaagcga tggttaaagc gtggcagaat
      241 attcctctgt taagctatat tttgttaaga ggacgctgtt accaatgcga tagtccaatt
      301 tcaattcgct acccttttgt tgagactctg accacagttt tatccttata cgccagttgg
      361 cattttgggt ttacaataca actattattt gctttattag ctatctggat attaatatca
      421 cttgttttta ttgatcttga tcatcaatta ctccctgata gcctcacttt agggctctta
      481 tggattggcc tcattgctaa tacccagaat atatttgtta gtcttgacgt tgctgtatta
      541 agctgcgctg gcgcctattt agccttatgg ttattcatta atctgtttta tctgatgaca
      601 ggtaaagtag gtatgggaca tggggatttc aaattatttg cagcatttgg tgcatggctt
      661 ggatggatgt atttaccatt aattctacta atatcttcaa taaccggggc tatcattggt
      721 ttgatttatt taaagataaa tggtaaatca agagatactg caataccatt tggccctttt
      781 ctttgcattt ctggattaat agctatgttt tggggagatt taattatcaa ttggtatata
      841 ggttattgga tgtaaaatgt tgattagctt agcgcaaccc aacaataaat agagaccaca
      901 cgcctaaaat gacatgagaa acggatactc ccaaaagaga tttctgtctg tgaaataacc
      961 acccccaaaa aagtccggcg ataaaagtta atgaggcaaa ggttagcccc aagtgagtgt
     1021 gcatagttgc aaagattaga ttggacagaa aaattccatt ccattgacga aataattttc
     1081 ccccgggtaa aaaatgaaaa aaagtagatt gtaacgcact tctggccatt agctcttgaa
     1141 tgggggcaaa aaggctatat aaaaaaatca tagcaaagta atattcccaa tccacttcat
     1201 tatttaccaa aaagcgtttt gttcctgaaa atataggggt attgtccaga tgatcgatac
     1261 ctattataaa aaaaaccttg ataattagta caaaaagcat aataggaatt gtaaaggcta
     1321 atgcctccaa gatggttttt ccccaatctt tcgcagtgat accaaatctg gaatatggga
     1381 ggccagtaaa gtgcattgca atgagaattg ctacagcaaa gattgagatt aagcagattg
     1441 atagtacggt agtgacagtt acctgatgta ttacagtaac caggaaatgt agagataaag
     1501 tatagaggca aataatccat agtagagcta tgataaatac ccccaatgta ttgcgccctg
     1561 atgcttcttt gagagcttgc tgcatatttt tcacagttac ttcatttgta aaacgaagtc
     1621 tcctggaaag tatttgagcc atatttttaa ttaacatcaa gttaagcttt ggtttagcct
     1681 ttaccacttc taatggatat tctataacac taagatcagt tttagccttt accgacgcag
     1741 aacgtcgact attttctata agagccattt ctcctacaac atcatctttt gataaggttg
     1801 caagaatatg gatattatgc tcatcaaact catcgatagt ttttttaaat atttcggcaa
     1861 caccatcgat tataataaag aatgagtcag cgagatcatt ttcattataa acaatgcttc
     1921 cagcagtata gtgcttatgc tgaccaattt ttgaaaaact ggcaagttca tcttttgaga
     1981 aaccaatcgt tatcgattgg taacttccta ttaattcagt atccaattga ttggagttgt
     2041 cattatcttt tttagaagca ttattttggt tcattgctat ttcaatccta aatatgcaag
     2101 tccacattta ctctatttaa tgcaacgaca atataaacct cataaataat ttttgattag
     2161 gaaattaata atgaataaat tttaagatgt tccctttctt ctttgcataa tagctgagaa
     2221 aaattctggt ttatttaatc ccattgatgc gaatatttga ggtattttat cccaacttcc
     2281 ctcttgttca atactgtcca caaccaactc gaatccagca ttcttatcgc tttcaacacc
     2341 ccagccattg ataatacata gtcctcttaa tctcttggca cctacatgtc cgagtttttc
     2401 agctgcgatt aaatgtgcat gtgcctcatc aaaaagttgc tgtgctcttt ttaagttggc
     2461 ttgactatta aatatacctt cattatccag tttttgtctg tattttgctt cgtccagaaa
     2521 aatttggcct agctgaaaat tagctgctga gtcatcgaga ttagctgctg ctctatagca
     2581 ctcaatcatc atgatttctg catatgggta ttttttatga ttctttaatt ttttaaaaat
     2641 tgtagctaat tcaaaataat aaaggatttc ttttttcaga acttcatccc caggttggtt
     2701 acttattcta ttggcttgca tagccttgat tctttttgtg atttttctta ttttccatcg
     2761 tttaaagata aacatgccct actcccttac ctaagctgtt aggtcaaata aaatctgtag
     2821 taaaaaaatt atcgcaacaa ttaaaaccat taagagaatg attttaagtc ctaatatagt
     2881 cgaatctcgc ccacgaccag caggcttgga agacgatttt tcttgcgtaa cttctaaagg
     2941 ttcttcctga agctttaaat
//
LOCUS       DATNSN010000252         2864 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.253, whole genome shotgun sequence.
ACCESSION   DATNSN010000252 DATNSN010000000
VERSION     DATNSN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.253"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(8..2020)
                     /gene="ravR"
                     /locus_tag="VAH76_003018"
     CDS             complement(8..2020)
                     /gene="ravR"
                     /locus_tag="VAH76_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavR"
                     /protein_id="HEO1387594.1"
                     /translation="MLSQGVRSMGKEKRELEASYIPLPKKGRFWIHSESCYTEEELNF
                     REWILKIRSNSGIHPDYLNNPSSWFANDDQALVNLYEHAWFYSNAYKPRHVLSLDDFH
                     VQPIDNQRLQWPQLYDLMQKVGDYLLLRNKELPPEHLNQPMNFVLLDVNNILKGLSQN
                     NNIQQVKEQLESVTRYLRTIEKNISPLIGSDRLFLANFRKTVDTKIHPHLDHMLETQL
                     LRDKLGELSKDIRKLSSERNRILHYALNANSVNPHSYEFELDKISDSASYPTQAAKHC
                     AQKSNELVADPLPILHLTKEQLSDCPHFKLITMKDEILDHYAKAVSDLNELERFQKVI
                     QEIMDLLGQAGEVYTVHQFKNQMQSLLTQIDSFIDASSVHIDEIIDANTQAYHKAIQE
                     EQNLPFWKNWLTNEKEKLKIFIKNQDTLSQFPSSSSDLKKTNKALKNRVNEVVSHLNQ
                     PKLKETSFEAIAGQTQALNKLMLSMHDWMKIQHELKGLPLPQPPEPLKIMPTPIPTVD
                     VFSSPPPPLHAYKPLFTALPGNTTAPCTPDNNQCKTTPSSPTQNSLGYLGLIALIPIG
                     VIALYLVIHWMNKKDAQIRVSGTEEEFNKIKEKFEDLLAELKGFEHSEDIDLSDDYYF
                     IVQDYEELIKKTKSNHYDVEALQDTYQELEDLYEEYCNPNKESLNF"
     gene            2280..2765
                     /locus_tag="VAH76_003019"
     CDS             2280..2765
                     /locus_tag="VAH76_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEO1387595.1"
                     /translation="MPSFDIVSKMNEVDLRNAVDNAVREVSTRFDFRGVEATIELKDL
                     TVTLRSESDFQVRQLEDLFRNHCSKRNLSTSGVDIEDEPVHSGKFYTLTMTFKQGIDQ
                     PTAKEIVKYIKETKAKVQTSIQGDKVRVTGKKRDDLQETIALLKKSNIELPLQYENFR
                     D"
BASE COUNT          849 a          465 c          571 g          979 t
ORIGIN      
        1 aattgagtta aaagttcagt gattccttgt taggattaca atactcttca tagagatctt
       61 ccaattcttg ataagtatct tgtaatgctt ctacatcata atggtttgat tttgtctttt
      121 ttatcaattc ctcatagtct tgtacaatga aataatagtc atctgagaga tcaatatctt
      181 ccgaatgctc gaaaccttta agttcggcta atagatcttc aaatttttct tttatcttat
      241 tgaactcttc ttctgtgcca gatactcgta tttgagcgtc ttttttattc atccaatgaa
      301 taaccaaata taaggctatg acaccaatag gtatcagggc aattaatcct aaataaccta
      361 aagaattttg tgtgggtgaa ctgggggttg ttttgcattg attattgtca ggcgtacaag
      421 gagctgttgt attgccaggc agggcagtaa atagaggctt atatgcatgg agtggaggcg
      481 ggggagaaga gaacacatca actgttggta taggagttgg cattatcttg aggggttcag
      541 gtggttgggg aagtggaagc cctttcaatt catgttggat tttcatccag tcatgcattg
      601 acaacatcaa tttattaagt gcttgagttt gtccagctat tgcttcaaag gacgtttctt
      661 ttaatttagg ctgatttaaa tggctaacga cttcattgac ccggtttttt aaagccttgt
      721 ttgttttttt gagatcagaa gaactggatg gaaattgaga gagtgtgtcc tgatttttaa
      781 taaaaatctt tagtttctct ttttcattag tcaaccaatt tttccagaag ggtaaatttt
      841 gttcctcttg tattgcctta tgataggctt gagtgtttgc atcaattatt tcatcaatat
      901 gcactgagga agcatcaata aagctgtcaa tttgtgtcag cagggattgc atttgatttt
      961 tgaattgatg aacagtatag acttcgccag cctgccccag aagatccatg atttcctgga
     1021 tgaccttctg aaatcgttcc aactcgttaa gatcagagac tgctttggca taatgatcaa
     1081 ggatctcatc tttcatcgtg attaatttga agtgaggaca atcacttaat tgttctttag
     1141 ttaagtgcag tataggcaga ggatctgcga ccaattcatt tgatttctga gcgcagtgtt
     1201 tggctgcttg agttggataa gaagcggagt cactgatttt atccagttca aattcataag
     1261 agtggggatt taccgaattg gcattaagag cataatgcaa gattctattt cgttcactgg
     1321 ataattttct gatgtctttt gataactctc ccaatttatc tctaagtagt tgagtttcca
     1381 gcatatggtc cagatgtggg tgaattttag tatccactgt tttgcgaaaa ttggctaaaa
     1441 ataatcggtc cgaaccaatc agtggtgaaa tattcttttc aatagtacgc aggtagcgag
     1501 tgactgactc aagttgctct tttacttgtt gtatgttgtt gttctgactt agccctttta
     1561 agatattgtt cacatcaaga aggacaaaat tcatgggttg gtttaaatgt tcggggggta
     1621 actccttatt tcttaataaa agatagtcac ccactttttg cattaagtca taaagttgtg
     1681 gccattggag gcgctgatta tcaatgggtt ggacatggaa atcatcaagg cttaacacat
     1741 gtctcggctt gtaggcattt gaataaaacc aggcatgttc ataaagatta accaaagctt
     1801 gatcgtcgtt ggcaaaccag gaactgggat tattaagata atccggatga atccccgaat
     1861 tggaacgaat ttttaaaatc cattctctga aatttaactc ttcctctgtg tagcaacttt
     1921 cagaatgaat ccaaaagcgt ccttttttag gtaacggtat atatgaggct tcaagctctc
     1981 tcttttcctt tcccatactt ctcactcctt gagaaagcaa aaaaaaactt aaggtaattc
     2041 cagattaata ctagattagc aatagctttt tttgctgccg attaataata tccataacca
     2101 actgggtaat ttttattcca atttatttga taacattgtt tgtgcaatta tgcaatggtt
     2161 aatcaattga attattttga aacaactttg atttttgcaa gaaagaaaat ggaggtgttg
     2221 tttctttcac ctatccttgt tttttgataa attacaattt taattttaat ggattggtta
     2281 tgccttcttt tgatattgtt tctaaaatga atgaagtgga tttgcgcaat gcagtagata
     2341 atgcagtgcg ggaagtaagt acccgatttg attttcgagg agttgaggct actattgagc
     2401 ttaaagacct gacggttact ttgcgttctg aatctgattt tcaagtgagg cagcttgagg
     2461 atttattccg gaatcattgc tcaaaacgca acctcagcac atcaggagtg gatatagaag
     2521 atgaacctgt gcatagtgga aaattctata ctcttaccat gacttttaaa caaggcattg
     2581 accaacctac tgctaaagag atagtgaagt acatcaaaga aaccaaggcc aaggtccaaa
     2641 cttctattca gggggataaa gtgagggtga ctgggaaaaa acgagatgat cttcaagaaa
     2701 ccattgcttt attaaaaaaa tcaaatattg aattaccttt gcagtatgaa aattttcgtg
     2761 attaattaat atgaattcga tataaaaggc aaagaaaagc cttttatatc cttaaatgaa
     2821 cgatatcacg taaaatgtac agcatctata agaaattctc cttg
//
LOCUS       DATNSN010000253         2863 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.254, whole genome shotgun sequence.
ACCESSION   DATNSN010000253 DATNSN010000000
VERSION     DATNSN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.254"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..541)
                     /locus_tag="VAH76_003020"
     CDS             complement(<1..541)
                     /locus_tag="VAH76_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387596.1"
                     /translation="MAETPKPKSQTPTPKPKPKPKPKPTDEQPVEPGPQQSTPGVGSA
                     IGGFIKKGVGKLADNIGKRFGSNAPQPGAENKPAEGQKGEDNKKDPGMEMLDRYMKLV
                     EQWQGVINKPFYWVGGKISAGAKAGASYVVDSAVEWIKNKLAGPEEPKPSPNSSTQQS
                     SSSSSSSSSSSSSSSSTTPQ"
     gene            1180..1965
                     /locus_tag="VAH76_003021"
     CDS             1180..1965
                     /locus_tag="VAH76_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO1387597.1"
                     /translation="MKINDFRYMRRGKHLSELQSEEANLLAPVNQRGKGTERALLVLH
                     GFSSSPAVYRYLIPNLKNYDAILCPVLAGHAESIEAFSKSKAADWLQSAKNACELLIN
                     QYQKVDVLGLSLGGLLACQLSQSFAINHLFLLAPALKLHMRINGMLKLAQILKCLGFR
                     QLRNAAGNLITDEHAEIAYRKLPISTIIEMLTLAQQYKWLPPACSADLFLGIHDQVVA
                     SQQVEQLFCNLPNVKIHWLENSAHVLPLDNDLNEIIDCINKSK"
     gene            2107..2340
                     /locus_tag="VAH76_003022"
     CDS             2107..2340
                     /locus_tag="VAH76_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387598.1"
                     /translation="MFFTKTSLKNDDLNVSKIAIFVAGLASLLIIGNYNDGDTYEEVS
                     PSFLSQIMNDPPNYPFTSDEEIFEVNIGLPFRF"
     gene            2618..>2863
                     /locus_tag="VAH76_003023"
     CDS             2618..>2863
                     /locus_tag="VAH76_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEO1387599.1"
                     /translation="MHKKIFCQNLLDSALKNSIHAKRKQYLVRFLSDLMDYDTTLSVT
                     EIGKKLTSKTTVKSKIYAAQTFVNNFKLERDIVCIYKS"
BASE COUNT          900 a          537 c          484 g          942 t
ORIGIN      
        1 gttgcggagt tgtcgaactg gaactggaac tggaactgga actggaactg gaactggaac
       61 tggattgttg cgttgaggag ttggggcttg gtttaggttc ctctggtcca gccagtttat
      121 ttttaatcca ttccactgca ctatctacaa cataactggc accggctttg gcgccagctg
      181 atattttacc acctacccaa taaaaaggct tgttgataac accctgccat tgctcaacca
      241 atttcatata cctgtcaagc atttccattc ctggatcttt tttgttgtcc tccccctttt
      301 gtccctctgc gggcttattt tcagcaccag gttgaggagc atttgagcca aatcgtttgc
      361 cgatattgtc agctaactta cctaccccct ttttaataaa tcctccaatt gcactaccca
      421 ctcccggagt actttgttga ggacctggct caacaggctg ctcatctgtt ggtttcggtt
      481 tcggtttcgg tttcggtttc ggcgttggtg tctgtgattt gggttttgga gtttctgcca
      541 tgttaattta ccatatctgt tgatatctaa attatagtac ctcatcaaga ttttgtccga
      601 ggttactttt cctagtttac tgttcaggaa tatatttttt atttaccata tatatatata
      661 gtaaaacaat tggatcaatc aaatacaata tgatctaaca atttacaaaa aatttactaa
      721 attaaaatca tatctgtaac tctcaggact tatgaaaaac cccaaggtca aggcaaacat
      781 gtttttagtg agggggttta gataaactaa atgaccgaat taaaaacatt ttttaacata
      841 gagattgggg tttttcgtaa gtcctgactc tataaactaa ctgtaattat taatcttaat
      901 ttctattcaa ttaaacttta gataatttcc attaaaacag tgcctttaca tattttgcaa
      961 aaaactattg ccccgaataa agcaaaaggg tttacacttt aaatttctta aatgacaaaa
     1021 aaaaatagta cagtacttat actaaaatta aactcataca attataactt ttacattttt
     1081 atatttggta atacacttcc cttttaggaa gtgtattacc cccaaataaa gtcaattatt
     1141 atgcaaccac atggcatgac aatgaacaat gaatactcta tgaaaatcaa tgattttcgt
     1201 tatatgcgcc gcggcaaaca cctcagtgaa ctgcagtcgg aggaagcaaa tctccttgct
     1261 ccagtcaatc agcgtggtaa aggcacagaa cgagctttac tggtattgca tggtttctct
     1321 tcttcaccgg cagtttaccg atatctgatt cctaatttaa aaaattatga tgccatcctt
     1381 tgccctgtat tggctggtca cgccgaaagt atagaagcct tttcaaaatc caaagcagct
     1441 gattggcttc aatcagcaaa aaacgcctgt gaactcttaa taaatcaata tcaaaaagtt
     1501 gatgttttag ggttatctct gggaggatta ttagcctgtc aattaagtca atcctttgct
     1561 atcaatcacc tgtttctatt agctcccgcg ttaaaattac acatgcgcat caacggcatg
     1621 ctgaaattag cccagattct taagtgcctg ggatttcgac aacttcgcaa cgccgcaggg
     1681 aatcttatca ccgatgagca tgctgaaatc gcttatagaa aactaccgat ttctacaata
     1741 attgaaatgc taaccttggc tcagcaatat aaatggctcc ctcctgcttg ctcggccgat
     1801 ctttttcttg gaattcatga tcaagtggtt gcttctcaac aagtagaaca attattttgt
     1861 aatttaccta atgtaaaaat acattggtta gaaaattccg cgcatgtatt gccactggac
     1921 aatgatctga atgaaattat tgattgtatt aataaaagta agtgaatgaa aatcaaatat
     1981 tgtcataatt gacttgcaaa aaatcataaa tcacgtcatt tggactttcc ctattataat
     2041 ttaaatattg aataaaaaaa aaatcattat attttatttg ctcactttgt tttttaggaa
     2101 ttaactatgt tttttactaa aacctcatta aaaaatgatg atttaaatgt ctctaaaatt
     2161 gccattttcg tcgcgggatt agcctcgttg ttaatcattg gaaattataa tgatggggac
     2221 acttatgagg aggtatctcc ctcatttctg agtcagatta tgaacgatcc ccctaattac
     2281 ccattcacct ctgatgagga aatatttgaa gtcaatattg ggctcccatt ccgattttaa
     2341 tcaaacaaag ctaaattcac caagggatta ccctaatgaa tttagctctc ttatcctgaa
     2401 taaatgttag gtaaccgtac acggggtatg caaatatttc tgtttataag cctatttatt
     2461 ttagctgtct ttcccgcgaa ggcgctgagg aatcctgtca tttaattgag cagttacttt
     2521 tttaaagtgt acattaacaa agatgggaaa agaaattgaa gtgcatacag attcatgatc
     2581 aaatttgttt accaagacaa aactaatcgg aagttgtatg cacaagaaaa tattttgcca
     2641 gaatttattg gatagcgcat taaagaattc aattcatgct aaaagaaaac aatatttggt
     2701 tcgcttttta agtgacttaa tggactacga taccacatta tccgttacag agattggtaa
     2761 aaaattaaca tccaaaacga cagttaaaag taaaatatat gcggctcaaa cattcgtgaa
     2821 taatttcaaa ctggaaaggg atattgtttg catttataag agt
//
LOCUS       DATNSN010000254         2829 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.255, whole genome shotgun sequence.
ACCESSION   DATNSN010000254 DATNSN010000000
VERSION     DATNSN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.255"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(53..295)
                     /locus_tag="VAH76_003024"
     CDS             complement(53..295)
                     /locus_tag="VAH76_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387600.1"
                     /translation="MECFIEVSEPIIDVKFQLKKDTQKYLIDYILSYSQLNCKELAQI
                     LEASPMMLSQVLAGKEFLGPSKARNLFHYFSMLIGH"
     gene            complement(445..1926)
                     /locus_tag="VAH76_003025"
     CDS             complement(445..1926)
                     /locus_tag="VAH76_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387601.1"
                     /translation="MLSPEKMPKGIMPLYLIQAFSTFSYAILYSSLSLFLTKQLGLSN
                     ALSNSIVGLFLAFNYVLHLLGGVVGGRYLSNRALFLITTAIQTIGILLLALSVKSLLY
                     LGLSLFLVGCGLNTTAYNSILTQRFAPDDNRRDKAFFLSYSYMNVGFCAGYIISGFFD
                     YSNQYQTLLYASIIPNVITLLLMWSYWSNLNDRDTPLIKVTNKASLNLKKSFGWGIIL
                     ALIPFTLLCFHKAQFSNGVVVGLSVMMFFVILYLGYQQKSTLDKQKIMTFLVLTVTSI
                     LFWMIYFTGPMGVTLFIKNNVDKHLYHYEIATQWLVNINALVIIIGAPLLSTVLTQLQ
                     AKGYNVSITKQFTWAFLILAGSFFCLASGILFANKHGYVSVYWVMLHFTTQAIAELLI
                     GPVGYAMIGKISPPQLQGVLMGTWMMVSGVSASLSHYFSNAMTQGESTNPLLSNGDYL
                     HVFNQLGMWALLGAIFLYLIAKRIRPVIEQTKDSDPSEVLTTA"
     gene            2046..>2829
                     /locus_tag="VAH76_003026"
     CDS             2046..>2829
                     /locus_tag="VAH76_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387602.1"
                     /translation="MNKISRVSWDEIKHQVKQVNESIYHVIEEIKPGNTIPFFIARYK
                     FGEHFGIKNHAYLPTSSGQMEKIDSKHTDNELFNHLGYGKNSLPLGLILDKFCEWHYF
                     GENERIFPDCVQGPGAIFNMQVIFDEDQTVDNNVLSVSSGALSSFLLPNIGCKRKHAR
                     IQKYFNVTAPAPKSPYEHYLVFKEILKDKNTIDNWYSEILYFSEQFINEVKHNDKWLK
                     LKLYFSESLRKKLTQNTYDASCNDLFLSAKSINRFRPTPFIMD"
BASE COUNT          929 a          496 c          547 g          857 t
ORIGIN      
        1 agagataccc cttgcccttg cagagattct gggtggtaga attaaaagcg agttaatgtc
       61 caatcagcat gctgaaataa tggaataggt tgcgtgcttt tgaaggtcct aagaactctt
      121 ttccagccaa cacctggctg agcatcattg gactggcttc aagtatttgg gctagctctt
      181 tacagttcaa ttgactgtaa gacaggatat aatcaatcaa atacttttga gtatcttttt
      241 ttaattgaaa cttaacatca ataataggtt ctgaaacctc gataaaacat tccatgtaac
      301 atcctcactt tcatcttaat acggcatagt ccaattgaaa aacagtaaac catagccgta
      361 atatcgataa aattagtttt tttacatgga gaagatcaca ggtaaaatgc aactttgatt
      421 aatttattac ctttttgcct ctccttaagc ggttgttaga acctcggatg gatctgaatc
      481 ttttgtttgc tcaataacgg gtctgattct tttcgcgatc aagtaaagaa aaatagcacc
      541 taataatgcc cacatgccca gctggttaaa tacatgcagg taatcgccat tggatagcaa
      601 tggattcgtt gactcacctt gagtcatagc attggaaaag taatgagata acgatgctga
      661 tacccctgat accatcatcc aggtgcccat cagcactcct tgtaattggg gtggagaaat
      721 tttaccaatc atggcatagc cgacaggccc tattaataac tctgcaattg cttgggtggt
      781 aaaatggagc attacccaat aaacactcac atatccatgt ttattggcaa agaggatacc
      841 gctggcaaga cagaagaatg aacctgctaa tatcagaaat gcccatgtaa attgtttggt
      901 tattgataca ttgtatcctt ttgcttgcag ttgtgtgagt acagtggata acaatggagc
      961 gccaataatg atcacaagtg cattgatatt cacaagccat tgagtcgcta tttcataatg
     1021 gtacagatgt ttatcaacat tatttttaat gaatagggtt acacccattg ggcctgtgaa
     1081 gtagatcatc cagaacagga ttgatgttac tgtcaggact agaaaagtca taatcttttg
     1141 cttatccaaa gtcgattttt gttggtaacc aaggtataaa attacaaaaa acatcataac
     1201 gctgagtcct actacaactc cattgctgaa ctgggcttta tgaaaacaaa gcaaggtaaa
     1261 tgggataaga gctaatataa ttccccagcc aaaagatttt tttaaattca gggaagcttt
     1321 atttgtaact ttaattaagg gggtatcgcg atcattcaaa ttcgaccagt aactccacat
     1381 aagtaaaagt gtgattacgt ttggaattat gctggcatag agtagtgttt gatattgatt
     1441 ggagtaatca aaaaagccgc ttatgatata gccagcgcaa aagcctacat tcatgtagga
     1501 gtagctcaag aaaaaggctt tatctctgcg gttatcatcc ggcgcaaagc gttgtgtcag
     1561 tatgctgttg tatgcggtag tgtttaagcc acatcctacc agaaataaac tcagtccaag
     1621 ataaagcaag gacttaactg aaagtgccaa aagtaaaata ccaatcgttt gtatggctgt
     1681 ggtaatcaaa aataaggctc ggttacttag atatcgtcca ccaaccactc cgccgagtaa
     1741 atgcagaaca tagttaaatg cgagaaataa cccaacaatg ctatttgaaa gagcattcga
     1801 caaccctaat tgtttagtga gaaacaggga taatgaggaa tatagaattg cataagagaa
     1861 ggttgaaaat gcctgtatta aatacagggg catgattcct ttgggcattt tttctggtga
     1921 caacatattt tttccctaaa actattttgg aaggtgactc taacattaaa aatggataaa
     1981 cttaatccct ttctacaatt atcctgattt tcaagggatg ataattgatg tatgggtgtt
     2041 taggaatgaa caaaattagt agagtttcat gggatgaaat caagcatcaa gtaaagcaag
     2101 ttaacgaatc catatatcat gtcattgaag aaattaaacc tggcaataca atccctttct
     2161 ttatagctcg atataaattt ggtgagcatt tcggcattaa aaatcatgct tacctaccga
     2221 caagcagtgg tcaaatggaa aagattgaca gcaagcatac cgataatgag ttattcaatc
     2281 atcttgggta tggtaaaaat agtctacccc taggcttaat attggataag ttttgcgagt
     2341 ggcattattt tggtgaaaat gaacgcatct ttccggactg tgttcaggga cctggtgcaa
     2401 tttttaatat gcaggttatt tttgatgagg atcagactgt agataataat gtgctgtctg
     2461 tttcttctgg tgcactttca tcctttttat tgccgaatat cggttgtaaa aggaagcatg
     2521 cacgaattca aaaatatttt aatgtcactg ctccggcacc taaatcgcct tatgaacatt
     2581 atctagtatt taaagagata ttgaaggata aaaatactat cgataactgg tatagcgaga
     2641 ttctttattt ttccgagcag tttattaatg aagtcaaaca taatgataaa tggcttaagc
     2701 tgaaactata tttctcagag tcattaagaa aaaaactaac gcaaaatacc tatgatgctt
     2761 cttgtaacga tttatttctc agtgccaaaa gtattaatcg tttcagacct acgccattca
     2821 tcatggata
//
LOCUS       DATNSN010000255         2808 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.256, whole genome shotgun sequence.
ACCESSION   DATNSN010000255 DATNSN010000000
VERSION     DATNSN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.256"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..924)
                     /locus_tag="VAH76_003027"
     CDS             complement(<1..924)
                     /locus_tag="VAH76_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO1387603.1"
                     /translation="MKNAVINGTGSYSPERQMTNAELETMLDTSDEWIVTRTGISSRS
                     VAQEHETTSYMASRAAEQALEASGLDAEEIDLILVATCTPDYFFPSVACHVQHALGIK
                     RPIPAFDIGAACSGFVYAMDVAKQYIATGAAKHVLVVGSESMSRAVDWTDRSICVLFG
                     DGAGAVVLSASDRQGIMGSVLHSAYDSDKLLVLRNSTFEQDRATIGMRGNEVFKIAVN
                     IMGNIVDEVLEASHLKKSDIDWLIPHQANIRIIQAIAKKLSLPMSHVIVTIGNQGNTS
                     AASIPLALDYSIKNNQIKRDEILLIESFGGGM"
     gene            complement(921..1949)
                     /gene="plsX"
                     /locus_tag="VAH76_003028"
     CDS             complement(921..1949)
                     /gene="plsX"
                     /locus_tag="VAH76_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO1387604.1"
                     /translation="MKNITIAIDAMGGDHGLEIVIPACIRAVKNNPDLKLLLVGVQDK
                     ISASLKKHGMLSCQQFTIVHASEVVTMDELPSHALRNKKDSSMRIAINLVKEGRAQAC
                     VSAGNTGALMATARYVLKTLPGIDRPAIVSELPTMGGKTRVIDLGANVDSCAEHLFQF
                     AVMGSALIQAIENKPKPKIGLLNIGVEEIKGNDQVKRTAHMLAECSVMNYVGYVEGDH
                     FYSGDVDLVVCDGFVGNVALKASEGLAKLLLTVLKESFSRNWLTKIAGLIALPALKHL
                     KNRLDPSRYNGASLLGLNGIVVKSHGGANEVGFQHAIEQAVLEVKNNVVDLVRDQIND
                     FINQGLLL"
     gene            complement(1955..2146)
                     /gene="rpmF"
                     /locus_tag="VAH76_003029"
     CDS             complement(1955..2146)
                     /gene="rpmF"
                     /locus_tag="VAH76_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO1387605.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTKPTLSVDPTTGETHLRHHMTP
                     DGYYRGKKIIDAETAYEQE"
     gene            complement(2232..2660)
                     /locus_tag="VAH76_003030"
     CDS             complement(2232..2660)
                     /locus_tag="VAH76_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HEO1387606.1"
                     /translation="MMHLQELAKHGQQEKEVILNERLPSFVSPGCHLHASYHIEAKDD
                     FYLIHLRVQGNLNSICQRCMENFTVCYNNSTVIAVCRSDERAEQLLSQYECLVSSNWQ
                     VDLNELIIDELHLYAPQVHPDINECANEINEILTGKKQTS"
BASE COUNT          871 a          603 c          463 g          871 t
ORIGIN      
        1 cattccacca ccaaaggatt caattaataa tatttcatcc cttttaatct gattattttt
       61 aatagaataa tcaagtgcta aaggaataga agcagccgat gtgttacctt ggttaccaat
      121 tgtaacaata acatgtgaca taggaagaga taatttttta gctatggctt gtatgatgcg
      181 tatattggct tgatgaggta tcagccaatc aatatcagat ttttttaaat gacttgcttc
      241 taacacttca tcaacaatat tacccataat attaacagca attttaaata cctcattacc
      301 tcgcattcca atcgttgcac gatcttgttc aaaagttgaa ttacgaagga ctagtaattt
      361 atcagagtca taggcagaat gtaaaacact acccataatc ccttggcgat cacttgcgct
      421 taaaacaaca gcgcctgcac catctccgaa taagacacaa atagaacgat cagtccaatc
      481 taccgctctt gacatgctct cgctgcctac gacaagaaca tgtttggcag cccctgtagc
      541 aatgtattgt ttcgctacat ccatcgcata aacaaaaccg ctgcatgcag ctccaatgtc
      601 aaaagccgga ataggtcttt tgattcctaa agcatgttgt acgtgacagg caacgctagg
      661 aaaaaaataa tctggggtac atgttgctac taatatcaaa tcaatttctt cagcatcaag
      721 gcctgatgcc tctagtgctt gctctgctgc tctggaggcc atataagatg ttgtttcatg
      781 ttcttgagca acactacgac tactaatacc agtcctggta actatccatt catcgctagt
      841 atcaagcatg gtttccagtt cagcattagt catttgtctc tctggagagt aacttccagt
      901 gccattaata acagcatttt tcataacaac aatccttgat ttataaaatc atttatttga
      961 tcacgcacta aatcgacaac attatttttt acttcaagaa cagcctgttc aatagcatgt
     1021 tgaaaaccca cttcattggc tccaccatga cttttgacaa cgataccatt caacccgagt
     1081 agactagcac cattgtatcg cgatggatcc aaacgatttt ttaaatgttt taaggctggt
     1141 aaagcaatca aaccagcgat ttttgttaac caatttctgc taaatgattc tttaaggaca
     1201 gttaataaaa gctttgccaa accttcactt gcttttaatg ccacatttcc aacaaatcca
     1261 tcacagacca ctaaatcaac atcaccactg taaaagtgat cgccctcaac gtatcccaca
     1321 taattcataa cagaacactc agccagcata tgtgcagtac gcttaacctg atcgttaccc
     1381 ttgatttctt caacgccaat attaagtaaa ccaattttag gtttgggttt attttctatt
     1441 gcctgtatca atgcagaccc catcacagca aactgaaaca aatgctctgc gcaagaatca
     1501 acattggctc ccaaatcaat gactcttgtt tttccaccca tggtaggtaa ctcggatact
     1561 attgctgggc gatcaatccc cggtaaagtc tttaagacat atcgggcggt agccattaag
     1621 gcacctgtat tgcctgcact gacgcaagcc tgcgctctgc cttccttaac caggttaatt
     1681 gctattcgca ttgaggaatc ttttttattt cgtaatgcat gagatggcaa ttcatccatt
     1741 gtaaccactt ctgatgcgtg gacgatagta aattgctgac aggacaacat gccatgtttt
     1801 ttcaaagaag cactgatctt gtcctggact cctacaagca atagtttcaa atcagggtta
     1861 tttttaacag cacgaataca ggcaggaatc acaatttcca agccgtgatc cccacccatc
     1921 gcgtcaattg caatggtgat attcttcaag aaagttactc ttgttcgtaa gcggtttcag
     1981 cgtcaataat ttttttgcct ctgtaatatc catctggtgt catgtgatga cgaagatggg
     2041 tttcaccagt tgttggatct actgatagag taggcttggt tagtgcatca tgagaacgcc
     2101 tcatatcacg acgtgagcgt gattttttat tttgttgtac ggccattgta ttactcctaa
     2161 acaaattgca ttcataaagc gggaatatta ccgatatttt aacttcaatc caagtattaa
     2221 ttatcaaaat atcaagaagt ttgttttttc ccagttaaaa tttcattgat ttcattagca
     2281 cattcattaa tatcaggatg aacttgtggt gcatataaat gcagctcatc aattattaat
     2341 tcatttaagt caacctgcca gtttgaagaa accaagcatt catattgact caaaagttgc
     2401 tctgctcttt catcacttcg acaaacagca ataacagtgg agttattata acatacagtg
     2461 aagttttcca tacatcgctg acatatactg tttaaattac cttgcactct aagatgaata
     2521 agataaaaat catcttttgc ctctatatga taagatgcat gcaaatggca tccgggacta
     2581 acaaacgagg gtagacgctc gtttaaaatc acttcctttt cttgctggcc atgttttgcc
     2641 aactcttgta aatgcatcat agataattat ccgaaatata gaatgagttt gctctttgag
     2701 tcttgcaagt aataagcact tatagacggt ttatgactta ctaacataat caagttaaaa
     2761 tgataacgaa tccctaaaaa cgtggtatta tccaattata tgtaaaaa
//
LOCUS       DATNSN010000256         2726 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.257, whole genome shotgun sequence.
ACCESSION   DATNSN010000256 DATNSN010000000
VERSION     DATNSN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.257"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..442)
                     /locus_tag="VAH76_003031"
     CDS             complement(<1..442)
                     /locus_tag="VAH76_003031"
                     /inference="COORDINATES: protein motif:HMM:NF037531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387607.1"
                     /translation="MRIKRKIIRHGFFWLPNSKKEVKGTLIILDGGKISLRLENLLHP
                     QNELIKIIHGRIGETAITLVDSYCKVYNDLISMISGGYGFEMHVHEAIFGQFFPSEEI
                     TTVEVYFWLEGIDNWINEINHLDVSNKLNISKDYNFILQKHAGGI"
     gene            complement(799..990)
                     /locus_tag="VAH76_003032"
                     /pseudo
     CDS             complement(799..990)
                     /locus_tag="VAH76_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947829.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1196..2119
                     /locus_tag="VAH76_003033"
     CDS             1196..2119
                     /locus_tag="VAH76_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEO1387608.1"
                     /translation="MTIPTYQECMLPMLKVLKDNSNIKSTRLISLMGDWFHLSDDERE
                     ELLSSGRTTILRTRVHWAKVYLKQAGLVEYPKRGYVAITEKGIQVLQKNPSNIDTNYL
                     KKFPDFIRFIERSKTESQQVSNSEYSENYSPDEVLTRTFERMKNQTCEELLEVIKSSS
                     PQFFEKLVVQVIVSLGYGGSLQEAGQAIGKSGDGGVDGVIKEDRLGLELIYLQAKLKK
                     DDKYNVGRPDIQKFYGALQGKQAKKGIFITTTDFSNEAREYLKVIDKKIILLNGKELI
                     ELMWEYNIGLNVSATYEVKDLDLDFFKEELE"
     gene            2318..2665
                     /locus_tag="VAH76_003034"
     CDS             2318..2665
                     /locus_tag="VAH76_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387609.1"
                     /translation="MVRPLRIEYAGGLYHITSRGNRKEAIYLSDADRENFLSVLGDVC
                     LKYQWLCHSYCLMTNHYHLLIETPLGNLSKGMRYLNGVYTQKFNKSHKSIGHVLQGRY
                     KSILVTTQEFAEL"
BASE COUNT          935 a          443 c          484 g          864 t
ORIGIN      
        1 tgattccgcc cgcgtgtttt tgcaaaatga aattataatc tttacttata tttaatttgt
       61 tactcacatc tagatggttt atttcattta tccaattatc aattccctca agccaaaaat
      121 acacctccac agtagtaatt tcttcggatg gaaaaaattg accaaaaata gcctcatgaa
      181 catgcatttc aaagccgtat ccgccagata tcatgctaat caaatcatta taaactttac
      241 aatatgagtc cactaacgtt atggctgttt ctccaatacg accatgaata atcttaatta
      301 attcattttg tggatgcagt aaattctcta gtcgtaaact gattttgcct ccatcaagaa
      361 ttattagagt tccctttact tcttttttgg agttaggcaa ccaaaaaaaa ccatgtcgta
      421 taatttttcg ttttattcgc attcatgatt ccaatcaaat cttctactct taagtatagc
      481 ggtttgttat cttaaataag cctcttctaa attcacaatt tgtgccttac aaattatttg
      541 aaataaaaac aacagaaatt gatataattg gatgaaacta ataagttata tgatacacag
      601 caaagttgat gggcaacttt gcggacaact ttaaaagaga tatataataa attccattat
      661 aaacaacatg ttaactgata agttaaatag ccgccgcccc accaatcatt aaaatcattt
      721 ctgaccactg aagaccatca taaacccttg tagatcaaca agttaaatcg gatctttccc
      781 aaatatgggg gcactttatc aagcacataa gactctaact tttaatcggt agttttcaaa
      841 gtttctaaac ccataagctc tacgttgtat tcatttcatt ttctgatgaa atccttcagt
      901 gatgccattt gatttactaa attgccacat acacgcaatt acttctttcc ttgaatcgag
      961 ggtttttccc aaggcaacta atgatttaaa acacacacgt acagggtcag atcttgcttt
     1021 tgctttctct aaaatgactc aaaactactt tgataggcaa aaggtagatc catgtaaata
     1081 gataaataac gaatattgtc tttttaaact tgatttatag tgattcatta agaataaaca
     1141 aatgagaagt cttggctata cttatttcaa cgagttgaat attaaacgga attttatgac
     1201 gataccaact tatcaagaat gtatgctacc tatgttgaaa gttcttaagg ataatagcaa
     1261 tataaaatcc actagattaa taagtctgat gggtgattgg tttcatttgt cagatgatga
     1321 gcgtgaagag ttattatcca gtgggagaac aacaatatta agaacaagag tgcattgggc
     1381 caaagtatat ctgaaacagg ctggtttagt tgaatatcct aaaagaggtt acgttgctat
     1441 aacagagaag gggattcaag tattgcaaaa aaatccctcg aacattgata ctaattatct
     1501 taaaaaattt cctgatttta ttagatttat tgagagatca aaaacagaaa gtcaacaggt
     1561 tagtaatagt gaatatagtg aaaattattc ccctgatgaa gtattgacta gaacttttga
     1621 aaggatgaaa aaccagacat gtgaggagct tctggaagta ataaagtcaa gttctcctca
     1681 gttttttgaa aaacttgtag tgcaggttat agttagtctc ggatatggtg gctcactaca
     1741 ggaagcaggc caagctatag gaaagtctgg agatggggga gttgatgggg taataaagga
     1801 agacaggctt ggtttagagt taatttattt acaggcaaaa ttgaaaaaag atgacaaata
     1861 taatgtgggg cggcctgaca tacaaaagtt ttatggtgct ttgcagggca agcaagctaa
     1921 aaagggaata tttatcacaa ccactgattt ttcgaacgaa gctagagaat atctcaaagt
     1981 tatagataaa aaaattattc tattgaatgg taaggaattg attgaattaa tgtgggaata
     2041 taatatcgga ttaaatgttt ctgcaactta tgaagttaaa gatttggact tggatttttt
     2101 taaagaagaa ctcgaatagt atgttaccta aactttttta ggcttgggta gttgcttaca
     2161 aaaactattt attcttacac gtggcgcacg tacagggtca gatcttgctt tttgccttta
     2221 tccataaaag acgacaagct tggaattacc gcttgataat tatttcccaa tgcattaatt
     2281 ttctttcaaa aggaattaag caagtccagg aacagcgatg gtaagacctt tgcgtattga
     2341 gtatgctggt gggctctatc acatcaccag tcgagggaat agaaaggaag cgatctattt
     2401 atcagatgca gacagagaga actttttatc tgttttgggt gatgtttgtt taaaatatca
     2461 gtggttatgc cattcgtatt gtctgatgac aaatcattac catttactta ttgaaactcc
     2521 gctcggtaac ctttcaaaag gaatgcgcta tctgaacggg gtatacactc agaaatttaa
     2581 taaatcacac aagagtattg ggcacgtcct gcaaggtcgc tataaatcta tattggtcac
     2641 tactcaagaa tttgcagaat tataagacaa cattatatgc aggaggcaaa ggacaagatc
     2701 tgacctacgc tctaaactat aataat
//
LOCUS       DATNSN010000257         2719 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.258, whole genome shotgun sequence.
ACCESSION   DATNSN010000257 DATNSN010000000
VERSION     DATNSN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.258"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(67..366)
                     /locus_tag="VAH76_003035"
     CDS             complement(67..366)
                     /locus_tag="VAH76_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387610.1"
                     /translation="MFSGNPQKGYLLMSDSAGKLKTPKFKKLRGKLWEYLKEFSALPI
                     KHGLELVIKQIKRSDTVVSRNQGQNYGKLFDDAKRRKNYQHTSDLMLFRGIFISK"
     gene            complement(377..688)
                     /locus_tag="VAH76_003036"
                     /pseudo
     CDS             complement(377..688)
                     /locus_tag="VAH76_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948078.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
     gene            complement(704..961)
                     /locus_tag="VAH76_003037"
     CDS             complement(704..961)
                     /locus_tag="VAH76_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387611.1"
                     /translation="MINLLDEHQNLGYIKKLISFVTLKLNVRPGKYLKKTVDLFSMPG
                     LLILSHESKEQVESDYNLVRKLEQKGLFVLAASQESKTTKI"
     gene            1137..1610
                     /locus_tag="VAH76_003038"
     CDS             1137..1610
                     /locus_tag="VAH76_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387612.1"
                     /translation="MRSRTEKLSSKRSIINHDHLITDEACMIGIYREGGDLSIYAESS
                     EGIEYFSINRGNSFDMVILPHTLVGTWRHHQLLRTVEQAAQIRALRIRSHDYIEQSVT
                     RTELEQFKEVFVLACKEEISNQPEIEEYLQTALGKLHGEPEMTYKPKFTEQCSIL"
     gene            1799..2716
                     /locus_tag="VAH76_003039"
     CDS             1799..2716
                     /locus_tag="VAH76_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387613.1"
                     /translation="MDKLNLNLLRALYALLTCRHLTLASKKMGISQSSMSIYLKQLRE
                     HFHDDLLVRGQGNIMQLTPLANSLIGKARQALSLIETLFNTIETFNPLISERSFSIGM
                     TDLMSILLTPSLIKRLEKEAPGVKLNIKHPSYLNTVEVFESDDIDLMIGMFERVPEGL
                     KQQKLFQDEAVIVGGSNHPGIQKGKICIEELFKYPLIQFSLRETPFYNYFDRYLRQLG
                     HDKRVSISLGQGIMPLLVLTDSHYLTLSIRKVAEKLSHLIPLSIASVPFEVDSFNCYQ
                     YWHQKDDADPGHRWLREMVLSIAKKSYLK"
BASE COUNT          896 a          501 c          432 g          890 t
ORIGIN      
        1 tcggtccaaa ctatctcatt attgaatagt catgtattaa atacctctat gtttcataca
       61 taacagtcat ttagaaataa atattcccct aaacaacatg aggtcagaag tatgttgata
      121 attttttctt cgcttcgcat catcaaacaa ttttccatag ttctgccctt gattcctaga
      181 tacaacagta tcagatcttt taatttgctt gatgactaat tctaaaccat gcttaatcgg
      241 cagtgcagaa aactctttga gatattccca tagcttgcct cttaattttt taaatttagg
      301 agtttttaac ttgcctgccg agtcactcat caataaatat cctttttggg gattacctga
      361 aaacataaat caatttttaa atactaccag ctttctctat gacaagtatt cttgcagccc
      421 cttttggata agctacatgc tcacatcctt ctttcaaata acatatgtcc ccactatttt
      481 attctttttg attttctaca ccaccctcac ggtagtgcat aatcactttt ccagacagca
      541 caacaaaaac ctcatcccca tcattcttat gccaaatata tggttgatcc gcccaatgaa
      601 gtctaatgct cgcatcagat atttgagcaa tatctaatgc ttgccatgca aaatcaccag
      661 taaataatga tgatttaatt atttgcatat taccttacgc ttattaaatt ttagtggttt
      721 tagactcctg gctagcagcc agaacgaata atcctttttg ctccaatttc ctaactaaat
      781 tataatcact ttcaacttgt tctttcgatt catggcttag aattaataga ccaggcatgg
      841 aaaaaagatc gactgttttc ttaagatact taccaggtcg aacgttaagt ttaagagtta
      901 caaaactaat taatttttta atatacccta gattttgatg ttcatctaat aagttgatca
      961 tctacatcac cattcatcct ccagaatttt aatactcatt attaaaattt cattaagaat
     1021 tttaagaata cgctaattat tcatcgataa aatgacactt atcaattcaa taagattaaa
     1081 aattattcat attgaagcga tgaatttatt aaataattgt aaaataggag ttatccatgc
     1141 gtagcagaac agaaaaatta agcagtaaac gttcaatcat caatcatgat catttaataa
     1201 ctgatgaagc ctgtatgata ggaatttata gagaaggggg tgatttatca atttatgctg
     1261 aatcatctga aggaattgaa tatttctcta taaatcgggg aaacagtttt gatatggtga
     1321 tattaccaca tactttagtt ggaacttgga ggcatcatca attacttcgt acggtggagc
     1381 aagcagcaca gattcgagca ttaagaataa gatctcatga ttacatcgaa caatcagtca
     1441 cccgaacgga acttgagcaa ttcaaagagg tatttgtgct cgcatgcaaa gaggaaataa
     1501 gcaatcaacc tgaaattgaa gagtatctcc aaactgcttt gggtaagtta catggggaac
     1561 cagagatgac ttacaaacca aagttcacag agcaatgcag cattctctaa tttttattag
     1621 aataatcaga gaaaaagctc gagattcaaa atcttcgagc ttttttaata tccttcatca
     1681 gttcgattag tgccaggctt atttgcaaac tgtatcgttc tcaactaacc tcaaacaaaa
     1741 agtcgttaaa aatagagtaa tttaaaacct attaggtcct atatacggat gatgttttat
     1801 ggataaactt aatctcaatt tacttcgcgc tctgtatgca ctattaacct gccgtcatct
     1861 aacacttgct agcaaaaaaa tgggtataag ccaatcctca atgagtattt acttgaagca
     1921 actgcgtgag catttccatg atgatttgct tgtacgtggc caaggcaata ttatgcaatt
     1981 aactccttta gctaactcgt tgattggtaa agcccgacaa gctctctcac tgattgaaac
     2041 actatttaat actatagaga cctttaaccc cctaatttca gaaagaagtt tctcaattgg
     2101 catgactgat ttaatgtcta ttttgttgac tccaagtttg attaaacgat tggaaaaaga
     2161 ggctccagga gtcaagctaa atatcaaaca ccctagttat ttaaatacgg tggaagtgtt
     2221 tgagtccgat gatattgatt taatgattgg tatgtttgag agagttccag aaggcttaaa
     2281 gcaacaaaaa ttgtttcagg atgaagccgt catcgttggg ggaagcaacc atccgggaat
     2341 ccaaaaaggg aaaatttgca ttgaagaact atttaagtac ccattaattc aattttcatt
     2401 aagagaaact cctttttaca attatttcga tcgctattta cgtcaactgg gacatgacaa
     2461 acgagtttct attagcttag gacaggggat catgccttta ttagttctta ctgactctca
     2521 ttatcttaca ttaagtattc gaaaagtagc tgaaaagctt tcccatttga taccattaag
     2581 tattgcttct gtaccctttg aggttgatag ttttaattgc tatcaatatt ggcaccaaaa
     2641 agatgatgct gatcctggcc atcgttggtt acgggaaatg gttctcagta tagctaagaa
     2701 atcttatcta aaataatca
//
LOCUS       DATNSN010000258         2668 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.259, whole genome shotgun sequence.
ACCESSION   DATNSN010000258 DATNSN010000000
VERSION     DATNSN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.259"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2178
                     /locus_tag="VAH76_003040"
     CDS             <1..2178
                     /locus_tag="VAH76_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SdbA"
                     /protein_id="HEO1387614.1"
                     /translation="KMYSVVRDKDNGLYDGVIHDHFCSIASLVDSQINSILYKLSTDQ
                     LLTEEEKQILCDDQLSHHFKPSQSVLKNPKYKGPHFISRQDLVAELGHREEYTNHDYF
                     LDRLREKFQLDRTIRPVALAEDGEKDIDGIPPQLSNHKERPLIIASSGGTGHISATHG
                     IINDLQSKTDNVVITQHHAELYKNKPFSITSVLIRIGVWLTSLPVLEDILKGVMRFIG
                     YPVLPSSSIFWDQMSKIQQSETKKENGIETGRTRPYVDMLLDIYPEGYEYTAFNNATH
                     LTSSIEDIQTMISFKGHVEEDNRNIVYQNILQRLMHAAKQSTPYTRLISTQALSLGAI
                     CDAVKYYNTVFLPVYNAERGTSYQPITIDQYMTDLPSLGCIHFMNNLEELTSEQRQLM
                     EIHAVNMSEPFKEAHFGKEQGFKAVHNIDPRNNPMIRNAFKDLSLTKYLDKTQSFDLH
                     FNVYKKEKQNALPVLNGKEKITIKPHAKIASIMIGSLAANASADYAQYLLNQGYDHIF
                     LFGGLNDSIAARIDQIINSYPDSRHDEIRKKIILLGNQSDVEMAPIMTRSNCVVIRGG
                     GLSVMEQMAMPIMDEKIVLLHHEDNEEGPLTSGLSWEDGNADKLIEYLSEKGAYAKKT
                     SPGLCSGHLNEAEKDFEKKYITQLKSKGTKEKVDLTIPQQKKPPLKKEWGRKTGYTEL
                     GHVLSHQHRFFETIPEVREPFCSKEDLHHHELSSQSLVSVTVG"
     gene            2312..>2668
                     /locus_tag="VAH76_003041"
     CDS             2312..>2668
                     /locus_tag="VAH76_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ras GEF/LegG2"
                     /protein_id="HEO1387615.1"
                     /translation="MKNKSNLLPLIACFNNREINMPKILDSNAYREMENELNNLFEQI
                     YKHGKNDRTAITLQVQQFLDNLSESIRDHSRLLLTNIAREELIYFKEKKPGDSREKFQ
                     NIDKMSDYFNNLSALIN"
BASE COUNT          875 a          478 c          540 g          775 t
ORIGIN      
        1 aaaatgtatt ctgttgtgcg ggataaagac aatggcttgt acgatggagt aattcacgat
       61 cacttctgtt ccattgcgtc gttggttgat tctcaaatta attctatttt atacaaatta
      121 tccactgatc agctgcttac tgaggaagaa aaacaaattt tgtgtgatga tcagctttct
      181 caccatttta aaccaagtca aagtgtttta aaaaatccaa aatacaaagg cccgcatttt
      241 atttcccgac aagatttggt agctgagctt ggtcatcgag aggaatatac caatcatgat
      301 tatttccttg accgcttgcg ggaaaaattt caattggatc gtacaatacg gccagtggct
      361 ttggctgaag atggggaaaa ggatatagat gggattccac cgcaactatc taatcataaa
      421 gagcgtccct taattattgc cagcagtgga ggaacagggc atatctctgc cactcatggc
      481 atcattaatg atttacagag taaaacagat aatgttgtca ttacgcagca tcatgcggag
      541 ctctacaaaa acaagccctt ctcaattaca tctgttctga ttcgtattgg tgtttggttg
      601 acctcccttc ctgtcctgga ggacatactg aaaggagtta tgaggtttat cggatatccc
      661 gttttaccga gctcctccat cttttgggat caaatgtcta aaatacagca aagtgaaact
      721 aaaaaagaaa atggaattga aacaggcaga acccgtcctt atgtggacat gttgttggat
      781 atatacccag agggatatga atacacagca ttcaataacg cgactcactt gacttccagt
      841 attgaagaca ttcaaacgat gataagcttc aaggggcatg ttgaagagga taacaggaat
      901 atagtctatc aaaatatatt acaacgcctt atgcatgcgg cgaaacagag tactccttac
      961 acaagattaa tttccacaca ggcgctttct ttgggagcta tttgcgatgc cgttaaatac
     1021 tacaatacgg tatttttgcc tgtatacaat gctgaaagag gaacctccta tcagcctatt
     1081 accattgatc agtatatgac cgatttgcct tcactgggtt gtattcattt tatgaataat
     1141 ttggaggagc tcacatcaga gcaaaggcaa ttgatggaga tacatgcagt aaatatgagt
     1201 gaaccattta aagaggcgca ttttggtaaa gaacaaggct ttaaagcggt tcataacatt
     1261 gacccacgta ataacccaat gattcgcaat gcctttaaag atctttcatt gacaaaatac
     1321 ctggataaga cacagtcatt tgatttgcac tttaatgttt ataaaaagga aaaacaaaat
     1381 gcactgccag tactgaatgg taaagaaaaa attacaatca aaccgcatgc taaaattgct
     1441 tccatcatga taggctctct ggcggcaaat gcttcagctg attatgcgca gtatttattg
     1501 aatcaggggt atgatcatat ttttcttttc ggcgggctta atgattccat tgccgcacgc
     1561 atcgatcaga tcatcaattc ttaccctgac tccaggcatg atgagatacg taagaaaatt
     1621 attctcctgg ggaatcaatc tgatgttgaa atggcaccga taatgaccag aagtaactgt
     1681 gtggttatcc gtgggggtgg tttaagtgtc atggagcaaa tggctatgcc cattatggat
     1741 gagaaaattg tattactaca tcatgaagat aatgaagagg ggcctttaac gtctggtctc
     1801 agttgggagg atgggaatgc cgataaatta atcgagtatc tatccgaaaa aggggcatat
     1861 gctaaaaaaa catcacctgg cttgtgttct ggccatctca atgaggcaga aaaggatttt
     1921 gaaaagaaat atattaccca actaaaatcg aagggaacca aggaaaaagt agatttaacc
     1981 attcctcagc aaaagaagcc tcccctgaaa aaagagtggg gtagaaaaac aggttatact
     2041 gaactcgggc acgtattatc ccatcaacat aggttttttg aaacgattcc tgaagtaaga
     2101 gagccattct gttctaagga agatttacat catcatgagc tttccagtca aagtttggtt
     2161 agtgtaacag ttggttgata aaatatgcct ctaatcatcg aggctcgttt tagtgcggcc
     2221 ttctctgaaa cttgtctgct caggatagta tcaatttgtg attggaatgc gtgctgaaca
     2281 atgcaactta aaatcatttt ttgtccacag tttgaaaaat aaaagtaatt tattgccatt
     2341 aattgcatgt tttaataata gagaaatcaa tatgcctaaa attttagata gcaatgcata
     2401 tcgcgagatg gaaaatgagc tcaataatct tttcgagcaa atttataagc atggtaaaaa
     2461 tgacagaacc gcgattacat tacaagttca gcaattttta gataatttga gcgaatccat
     2521 tagggatcat tcccgcttat tgcttaccaa tattgccaga gaagaactca tttattttaa
     2581 ggaaaagaaa cctggagatt cgcgagagaa atttcaaaat atcgataaga tgagtgatta
     2641 ctttaacaat ttatctgcat tgattaat
//
LOCUS       DATNSN010000259         2628 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.260, whole genome shotgun sequence.
ACCESSION   DATNSN010000259 DATNSN010000000
VERSION     DATNSN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.260"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..259)
                     /locus_tag="VAH76_003042"
     CDS             complement(<1..259)
                     /locus_tag="VAH76_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HEO1387616.1"
                     /translation="MGNFTDTYIRNLKGKDKRYEEYEGGGFGIRVTPNQLKSWIYRYK
                     IGKKTDKITLGHYPSMSLADARKKFIELSELRRSGQAPKKII"
     gene            976..1161
                     /locus_tag="VAH76_003043"
     CDS             976..1161
                     /locus_tag="VAH76_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387617.1"
                     /translation="MAYDDDLKKKKKESPTSLNIGADEETGNYYCPVDNNHNPIHEKR
                     KYIKVGEQIPSGWVQCD"
     gene            1441..1866
                     /locus_tag="VAH76_003044"
     CDS             1441..1866
                     /locus_tag="VAH76_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1387618.1"
                     /translation="MSYQFELVTDIKTLEEQNKFIRQGILSFNDSFMGGIPEQYSIYL
                     KDESNKIIGGAIVYAYISSIYVDVIWIEENYREKGLGTKLLNQVEAEALKRNIFVSTL
                     DTFSFQAEQFYLKQGYIHLGIIKNYLNGHDRIYLRKELA"
     gene            complement(1960..2463)
                     /locus_tag="VAH76_003045"
     CDS             complement(1960..2463)
                     /locus_tag="VAH76_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387619.1"
                     /translation="MSLYLAIGHIVGAFFVLFLFESGIALLAAYQMKKSEKLGLLELS
                     SFLGVDIADLYKEELPPGLAPRITAFFLDRFGNDLFINRISDLLGLILTIWNVLGLVI
                     KIVLFIYVVWLTITVSLDNAIYAWLIIPIAIFFWITSYIVSLLCWLITGRYPGQAKLV
                     RKIVANQ"
BASE COUNT          909 a          418 c          468 g          833 t
ORIGIN      
        1 gtataatttt tttaggggct tggccagatc ttcttaattc acttaactca ataaattttt
       61 ttctggcatc agcaaggctc atgctaggat aatggccgag ggtaatttta tcggtctttt
      121 taccaatttt ataacgatat atccacgatt tgagctggtt tggtgttaca cgaataccaa
      181 atcctccgcc ctcatattcc tcataccgct tatctttacc ttttaaattt cttatataag
      241 tgtccgtaaa atttcccata ttatatttcc tttttgggta cactttgggg tacacttaat
      301 tttgttctgg attgaaattt tttgcaatgg tttgatatcg attattttat aaagtccttg
      361 tgtggaaagg gttaagtgat aataaatgat gctggttgat atttaatgaa atcgttgtga
      421 attagtttcg taatcaatag gtccgcggtt cgattccgcg cagcggcatt gtaaaatcaa
      481 gtagttacga agttttcttg aagtaatcta tttttgtgtc gcagctgtgt cgcagttaaa
      541 tgggaaagag ccaaatattt taaatctggc taaattggaa tgagccacaa gataaatatc
      601 aaactcactt tggtttgctc ttttataatt ggctacagaa gaacaagccc catttaatgt
      661 cgtttcgttg cacaggagat aagtattaag taataagtat atgggtaaag gggtggcaaa
      721 gagatcgctc tgatgaatat taacttttca gttaatagta aaaaaatagt ttaatagctt
      781 acaccgtaaa atctgtttta atttaacttc tgattatcag aaaagactaa ttatagttgg
      841 tcgacagcaa tgtctccaaa aaccatatta ataaagctag cggtaggtca ggaatactta
      901 ataattcaga atctcaaaac aaatctcttt ctagactata attgttatga cagatattga
      961 ataggaacta cgtttatggc ttatgatgat gatctgaaaa aaaagaaaaa agaatcacca
     1021 accagcttga acattggtgc agatgaagaa acagggaatt attattgtcc agttgataat
     1081 aatcacaatc caattcatga gaaaagaaaa tatattaaag tcggtgagca aattccttct
     1141 ggctgggttc agtgtgattg atatggttat aatctagctt aaggcaatcc gtaatgtgaa
     1201 aaaatgaatt ctaagatccc atattgcata aaaggttttc atgtctttaa aaaaggtcaa
     1261 accaagggag attggaaata tttcaacact tgaaaaccaa tctatttagc ctctaaatag
     1321 gaaggctcgt aaaaaccgga ttttttcctg ctcaatgtgc cagactataa gtgccccaaa
     1381 acacataaat atgtgaataa tcatcatgta atgtaaaatg atgaaaaaga ggattaatac
     1441 atgtcttatc aatttgaact tgtaacagat attaaaacgc ttgaagaaca aaacaaattt
     1501 atacggcagg ggattttaag cttcaatgat tcctttatgg gtggcatacc ggagcagtac
     1561 tctatttatc taaaagatga aagtaataaa attataggtg gtgctattgt gtatgcttac
     1621 ataagttcta tttatgtcga tgtgatatgg atagaggaaa actatagaga gaaaggtctc
     1681 ggcacaaaat tacttaacca agttgaggca gaggcattaa agcgaaatat ctttgtgtcc
     1741 actttggata cgtttagttt tcaggctgag caattttacc tcaaacaagg atatatacac
     1801 cttggaatca taaaaaatta cttaaatggt catgatagaa tttatttaag aaaagaactc
     1861 gcttgagaaa ttgtaattca ctttaattaa gatactagga caagattcgg aacatcccaa
     1921 taacccaaag tttggtggtc taaatttctg gaaataatac tattgatttg ccactatttt
     1981 tcttactagt tttgcttgac caggatatct tccagtaatc agccaacata ataaagaaac
     2041 aatataactt gtaatccaga aaaatatggc aataggaata ataagccatg catatatagc
     2101 attatctaag ctgacggtaa tggttaacca aacaacataa atgaacagaa ctattttgat
     2161 aactaatcct aatacattcc atatggttaa tattaagccc aacaaatctg atattctatt
     2221 tataaataga tcattgccaa atcgatcaag gaaaaatgct gttattctag gagctaggcc
     2281 aggaggtaat tcttccttat ataaatcagc tatatcgaca ccaagaaatg atgaaagctc
     2341 tagcaggcca agtttttcac tttttttcat ttgatacgct gctagcaatg cgatccctga
     2401 ttcaaataga aagagaacaa agaatgctcc tacaatatgt ccaattgcaa gatataagct
     2461 cattatccat ccttgattta aatgagttaa ccacgagtat atgaataatt ttagctcgat
     2521 atgtataata tatccaacaa taagtggggt ctcgattgag atggaacttt taacgggctc
     2581 cgcactctag aacttgcgcc ctgatgacga taagacccct gcaccagg
//
LOCUS       DATNSN010000260         2570 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.261, whole genome shotgun sequence.
ACCESSION   DATNSN010000260 DATNSN010000000
VERSION     DATNSN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.261"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..268
                     /locus_tag="VAH76_003046"
     CDS             <1..268
                     /locus_tag="VAH76_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387620.1"
                     /translation="IFASDTIKITVNTKEKNAIAIGYSVNGNQYGGLGSSYSGHGPKN
                     KEYQFGYKKDSIFGKDISCGSMTLNQDSKVTLVNNNNQCISVLG"
     gene            533..682
                     /locus_tag="VAH76_003047"
     CDS             533..682
                     /locus_tag="VAH76_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387621.1"
                     /translation="MKKIILAVVVISAAVLLSGCGGGCCGASNSCCSYNTGCCGVQNY
                     SGIWY"
     gene            714..1616
                     /locus_tag="VAH76_003048"
     CDS             714..1616
                     /locus_tag="VAH76_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO1387622.1"
                     /translation="MKKNSLLAISEEGFHHIAYTEWGIFEPELPTVICVHGYTRNSRD
                     FDELANYLSSKGRHVFCPDIAGRGDSSWFKNPRYYNFTQYIKDMTVLMARTNAHQIDW
                     IGTSMGGIIGMILAAMPNSPINKLVLNDIGPQIPIHGLRRIAKYAGKDPDFKSLEEAK
                     QHFKASYADFGITNEEQWDIFTKNSVEQRGPNLYVTKMDPAIKRSKSILQIISEFFRH
                     PHKALEGIFYDINLWSIWKQIKCPVLVIRGVNSDILTSEIITQMKKTHALTEVYEVEN
                     AGHAPALLDLTIHQRIEDWLATHQ"
     gene            complement(1646..2548)
                     /locus_tag="VAH76_003049"
     CDS             complement(1646..2548)
                     /locus_tag="VAH76_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEO1387623.1"
                     /translation="MNSQEQRGSFYAILSGFLYGFIGYFGISAIHGDLSASNMLFWRF
                     LISSIFILIVLIPQLKNINDTYKSMFTAFLSGVLYYGLSTLLYFYASLYIGSGLSMVI
                     FFTYPVIIMVFNYFFYGQTIPKIYYLAISVILIGMALLIDVNEIAFDLWGIFLGLASA
                     FFYACYIVSSKNNEISPNVSTLMVCLGCMTTSLLVACLDQTFKVPTSFPVWLNLFGIG
                     LVATVIPILLMLHSLKYISSEKASILSVLEPVFVVIFGVMLLGEELKPWHALGIVLVL
                     AGALITLFSHRINLNQWLSAIYKS"
BASE COUNT          921 a          462 c          469 g          718 t
ORIGIN      
        1 catttttgcc tctgacacca ttaagattac agttaatacc aaggaaaaaa atgccattgc
       61 tataggttac tcagtcaatg gaaaccaata tggaggtttg ggcagttcct attctggaca
      121 tggacctaaa aataaggagt atcaatttgg atataagaaa gattccattt ttggaaaaga
      181 tatctcttgt ggttctatga ctttaaatca agacagtaag gtgactttgg taaataataa
      241 taaccaatgc atcagcgttt taggttaatt ggctattgca tggaatgtag atattaattt
      301 tgaaaagaca gtagtgccta tccaacaata ttccgctcaa aataattgaa ttcaactcgt
      361 agaaactcaa atctgtaatt aacagtttat cccggttaaa gagtacagcc ctctctccta
      421 aaccagacat ttagcaacca aaaataccaa aaaacacaat caatacaatc aattggataa
      481 ttttaaattc atatagtata tttaatatac atggaccaaa caaggatagg caatgaaaaa
      541 aataatattg gccgtagttg ttatttctgc tgctgtatta ttgagtggct gtgggggtgg
      601 atgctgcggt gccagcaatt catgttgcag ctataatact ggatgttgtg gtgtacaaaa
      661 ttattccggt atttggtatt gatttacaat tataaaaacc aatagtctga aatatgaaaa
      721 aaaacagctt gcttgccatc tcggaagagg ggtttcacca cattgcttat actgaatggg
      781 gtatctttga accggagtta cctacagtca tttgtgtgca tgggtatact cgcaattcca
      841 gggatttcga tgaattagcc aattatctta gctccaaggg acgtcatgta ttctgccctg
      901 atattgcggg tcgaggtgat agttcctggt ttaaaaatcc ccgctattat aattttaccc
      961 agtatataaa agatatgaca gtccttatgg caagaacaaa tgctcatcaa atcgattgga
     1021 ttgggacatc aatgggcggc atcattggca tgattttagc cgcaatgccc aattccccaa
     1081 ttaataaact tgtgctcaat gatatagggc ctcaaatacc tattcatggg ttgagaagaa
     1141 ttgctaaata tgcaggtaaa gaccctgatt ttaaaagcct ggaggaagcc aagcaacatt
     1201 tcaaagccag ctatgctgat tttggaatta ccaatgagga acagtgggat attttcacta
     1261 aaaacagtgt agaacaacga ggccctaatc tttatgtcac caaaatggat ccggcaataa
     1321 aaagatcaaa atcaatttta caaatcattt cagaattttt tcgtcatccc cataaagcac
     1381 tggaaggaat tttttatgac attaatctgt ggtcaatttg gaagcagatt aagtgtccag
     1441 ttttagttat tcgtggtgtg aattctgata tcttgacttc ggagataatc acacaaatga
     1501 aaaaaactca tgctttaact gaagtttatg aagtagaaaa tgcaggacac gcaccagctc
     1561 tattggatct gactattcac caaagaattg aagattggct tgccactcat caataaatca
     1621 caaaagaaat gccacatctt tattatcagg acttgtatat tgcgcttagc cattgattta
     1681 aattaattct atgactaaac aaagtaatca aagcgcctgc caaaaccaag acgataccca
     1741 acgcatgcca aggcttgagt tcctcaccga gcagcattac gccaaagatc accacaaaaa
     1801 caggttctaa aacggataag atagatgcct tttccgaact gatgtacttc aagctgtgca
     1861 gcattaacaa aatcggtata accgttgcaa caaggcctat accaaataaa ttcagccaaa
     1921 cagggaatga tgttgggact ttaaaggtct ggtcgaggca agctacaagc aaactggttg
     1981 tcatacagcc taaacatacc atcagggttg agacatttgg agaaatttca ttgtttttac
     2041 ttgaaacaat ataacatgca taaaaaaaag ctgaagctaa gcccagaaat ataccccata
     2101 aatcaaatgc gatttcattt acatcaatta acaacgccat tcctatcagg ataacagata
     2161 tggccaaata gtatatcttc ggtatcgttt ggccatagaa aaaatagttg aacaccataa
     2221 taatcactgg ataagtaaaa aatatcacca tcgataatcc agagcctata tagagggaag
     2281 cataaaagta cagtaatgtc gataaaccat aatagaggac accacttaaa aaagcggtaa
     2341 acatggattt ataggtatca ttgatatttt ttaattgagg aatcaaaaca atcaaaataa
     2401 aaatgctgga aataagaaat cgccaaaaca acatattgct tgctgataaa tcaccatgga
     2461 tagcactaat cccaaaataa ccaataaatc cgtaaagaaa tccggataaa atagcataaa
     2521 aggatccacg ttgttcttgg gagttcatga ttgagttact cgaaataaga
//
LOCUS       DATNSN010000261         2562 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.262, whole genome shotgun sequence.
ACCESSION   DATNSN010000261 DATNSN010000000
VERSION     DATNSN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.262"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            14..490
                     /locus_tag="VAH76_003050"
     CDS             14..490
                     /locus_tag="VAH76_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387624.1"
                     /translation="MSWVWSLYLISVGIVVIGLGYISDFIHKEKLLVAGYALQTIFTF
                     CYIFITSKKQLALLQIGLGISGGLSTTTWYALYARYEDKTHEGLTWGLANGLSSIYTG
                     IALLFGGLIIAKTSFDVLFIIMGCIQFLATIYVSKLIFIEHPKIIRRFRNPNLAKN"
     gene            654..1166
                     /locus_tag="VAH76_003051"
     CDS             654..1166
                     /locus_tag="VAH76_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387625.1"
                     /translation="MFESFRKCLSTISMRAPYASTPGHRNAQRANAGSEVLGTTLETF
                     DPAASIPTKLAQTLISIYSLFRFDTHVSEKLIHLLQGSIAATQMGLGIALLFTGTECE
                     EYTDADLCKAIFLLQLLYRGTLLVGWAPSEFSKDPYAEPKVVSEDKAESDSESEEENE
                     DEESRNSASV"
     gene            complement(1225..>2562)
                     /locus_tag="VAH76_003052"
     CDS             complement(1225..>2562)
                     /locus_tag="VAH76_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEO1387626.1"
                     /translation="NKISGQINGVETKRSPGSTLKPFIYGLALDQGLIHPNTVLKDVP
                     QSFNGYNPENFDYDFMGPIKAKDALVLSRNIPAIELSNQLTNPTLHKLLEQAHVTQLR
                     SESYYGLSLNLGGVELTMKELVGLYAMLINDGVWYPINSLKGVPRQKGRRLLSTEASF
                     LVFDMLKNTPMKESNNEGSSQLPIAWKTGTSSGYRDAWTVGVFGPYVLAVWIGNFDNQ
                     ANPAFIGKEIAAPLFFELIDAIKHERGPIARVEKYPEHMHLEKIEVCKASGMLPGRYC
                     TDTEWTWFIPGKSPIKTDTIFREVAINGKTGLRTCHIDENTRFEVYEFWPSDLLRIFR
                     RAGIQRHTPPFFEPGCALSGNSGISPQITSPQAGVSYIVHANTQSNKIIPLTAVTDAG
                     IAHLYWFINESFVAETKSDEPYLWQAKPGKYVVRVVDDRGLSDARDIIIQMVS"
BASE COUNT          766 a          515 c          467 g          814 t
ORIGIN      
        1 gtctccctta gacatatcat gggtatggtc tttgtattta atttcagttg gaattgtagt
       61 aattggcttg ggttatattt cagattttat ccataaagaa aaattgctgg ttgcggggta
      121 tgcactccaa accatattta ccttttgtta catctttata acaagtaaaa aacaactggc
      181 tttattgcaa attggccttg gaatatcagg tggactctct actacaactt ggtatgctct
      241 gtatgcccgc tatgaagata aaacgcatga aggactcacc tggggtttgg ccaatgggtt
      301 atcaagtatt tacactggga tcgccttgtt atttggtggt ctaattattg ccaaaacatc
      361 atttgatgtt ttgtttatca tcatgggatg tattcaattt ctagcaacta tttatgtcag
      421 taagctcatc tttatcgaac accccaaaat tatcaggcgt tttagaaatc ctaatttggc
      481 taaaaattaa ttttaaaatc tgaaagccat gttgaaatgt caaatcaaat gaacattttt
      541 tgatgtagat tgatgctaaa ttcatcaaga aatactggaa tcaaaataac ttgtctgtta
      601 atgtccgctt ttattaaaga tatcacgtat taaattgtta taaggatatg attatgtttg
      661 aatcttttcg aaaatgtttg tctaccattt ctatgcgagc tccgtatgcc tcgacaccgg
      721 gacatcgcaa cgcgcaacgc gctaatgccg gatccgaagt attgggcaca acgcttgaaa
      781 catttgatcc tgccgcatca attcccacaa aactcgctca aactttaatc tcaatttatt
      841 ccctattcag atttgatact catgtttctg aaaaactgat ccatcttttg caaggtagta
      901 tagcggcaac acaaatgggt cttggcattg ctttgctttt tactggaaca gagtgtgagg
      961 aatatacgga tgctgatcta tgcaaggcaa tatttctatt acaattacta tacagaggaa
     1021 ccttgctagt gggctgggct cctagtgaat tttccaagga tccctatgct gagcctaaag
     1081 ttgtatcaga agacaaagcg gaatctgatt ctgaatctga agaagagaac gaagatgaag
     1141 agtcaagaaa ctcggcctca gtttaatttt aaatcccttg ctccgattag gacaagggat
     1201 cacttataca tcatcaaata tgcgtcaact aaccatctga ataataatat cccgcgcatc
     1261 agataaacca cggtcatcta ccacccttac cacatattta ccaggtttcg cctgccagag
     1321 ataaggttca tctgattttg tttcagctac aaaactttca ttgataaacc agtataaatg
     1381 tgcaattcca gcatctgtta ccgcagttaa tgggattatc ttgtttgatt gggtattagc
     1441 atggacaata taacttaccc ctgcctgggg agatgtaatt tgggggctaa taccactatt
     1501 tcccgacaaa gcacaacctg gttcaaaaaa tggcggggta tggcgttgaa tccctgccct
     1561 cctgaatatt cttaataaat cggagggcca aaactcatac acttcaaaac gagtattttc
     1621 atcgatgtga caagtacgaa gccccgtctt accgttaatt gccacctctc gaaaaattgt
     1681 atccgtttta ataggagatt tacctggaat aaaccaggtc cattctgtat ccgtacaata
     1741 tcgaccagga agcatacctg aggctttgca tacttctatt ttttccaaat gcatgtgttc
     1801 agggtatttt tcaactcgag ctattggtcc tctttcatgt tttattgcat caatcaattc
     1861 aaaaaataaa ggcgcagcaa tttctttacc tataaaagcc ggatttgcct gattatcaaa
     1921 atttcctatc caaactgcca atacataagg accaaaaact ccaactgtcc aggcatctct
     1981 gtatcctgag gaagttcctg ttttccaggc aattggtaat tgtgagctgc cctcattatt
     2041 cgactctttc attggagtat tttttagcat atcgaaaact aaaaagcttg cttctgtgct
     2101 aagcaagcgt cgaccttttt gtcgcggaac cccttttaat gaattgattg gataccagac
     2161 cccatcatta atcagcatag catataaacc gactaactct ttcatagtaa gttcaacacc
     2221 acccaaatta agagacaagc cataatatga ttcggaacgc aactgagtta catgcgcctg
     2281 ttcaagtaat ttatgtaatg ttggattagt taattgattg gaaagctcga tggccgggat
     2341 attacgacta agtaccaaag catcttttgc tttgatgggt cccataaaat cataatcgaa
     2401 gttttccgga ttataaccat taaaactctg tggtacatcc tttaaaactg tattggggtg
     2461 aattaaacct tgatcaaggg ctaatccata aataaatggt ttcagagttg agccaggtga
     2521 ccttttggtt tctacaccat tgatttgtcc acttatctta tt
//
LOCUS       DATNSN010000262         2511 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.263, whole genome shotgun sequence.
ACCESSION   DATNSN010000262 DATNSN010000000
VERSION     DATNSN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.263"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            273..>2511
                     /locus_tag="VAH76_003053"
     CDS             273..>2511
                     /locus_tag="VAH76_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidC"
                     /protein_id="HEO1387627.1"
                     /translation="MIELPLREPRNPRYLYIGPDNTLHVFMPIVGGTSIGTDNTCKAV
                     YSLQEFFGKGSNSNLQTTLKSELLAYQEALEHDLGLLGSETLLAHQKQERLTQIKAYL
                     EVLKNLEHHSELNCLNSGFPSYPRPLESMMQSRERANVYSMVLRPTQEDGYLRLESAN
                     PVFSVAHKSVARNIMATVSALQEALIQAYTPLRLEPKGLKYKAMRETMKQSSISRAPV
                     DFGRLRDVFQKRLQILMDDKSIDLTHTPDGTLVDQAYLDKAMVFNAQTTTPKEYMNAL
                     LGFCVPQLFATTLESPFDTLEHAERWSVATQFLLGLINIHGVTQGHLNPETNWGWILD
                     EHPDLSQSLAQTLAKAQQTKDSIESVCLAWINAHAHELKLNRSFNPQDLKQIKEDFAT
                     LYTQIEDSPHFDEFLVFRRDRKGDFVTHQASICTSFATFACHPLLGLPIEVTQPLERA
                     QAALGTLGTQIPHNPMSEKKITLDVAKMSLPEVQDLYERIATYKDPKVKAKLHAQLKQ
                     ERPDFKPQINAKQFLQCVAFGQQNDAEALLKEDTDRAQELLLADNMSFTDYSGRIFTC
                     TAYEYAYWAKDTHMCRMLEKYMDNTTKHDLLQRVQRIEELVGEGLFKAPRGLTYTQNG
                     EEHHSAHFDLTPLKQALKTYIDAYDQSPKQTDAEWEALDTLWVKVGLPQRDVPAHIAQ
                     EYCHPNRSFVEVSNNPSLLGATNPNNLMRQLKFYNWDTGATDSWFTPGSYSSNSGLGF
                     SFAILR"
BASE COUNT          726 a          608 c          539 g          638 t
ORIGIN      
        1 tttttgtatt ggagaaaatg gttatgagtc taatcgtttg tatttaccag cataatattc
       61 ctgacacatt tttcagattt taatgcagtc atgtttggaa agaggcttat taacgccatt
      121 ctcaactttt caagagtttc tcaaatgagt agagcccctt actgctaata atcaaaacat
      181 cgtatcgtga gtcatgtgaa gcctgtacag cagttcctgt tgcaatcaat ctttgctaca
      241 atataaatca aaataacaaa ataagagcca atatgatcga gcttccctta agagaacccc
      301 gtaatccccg gtacctgtat atcggcccag acaatacgct ccacgtattc atgcccatcg
      361 ttggcggcac gagcatcggg acggacaata cctgtaaagc ggtgtattcg ctgcaagagt
      421 tctttggcaa aggttctaat tcaaacctgc aaacaacgct caagagcgag ctgttggcgt
      481 atcaggaggc tttggagcat gatttaggtc ttttgggaag cgagacactt ttggcgcacc
      541 aaaaacaaga acgactgacg cagattaaag cctatcttga agtgctcaaa aatttggagc
      601 accactcaga attaaactgt ctgaactcag gctttcccag ttatcccaga ccgttggaat
      661 cgatgatgca gagccgcgag cgagccaatg tctattccat ggtgctgcgc cccacccagg
      721 aggatggtta ccttcggttg gaatcagcca accccgtttt ctctgtggcc cacaaaagtg
      781 ttgcccgaaa catcatggca acggtatctg cgttgcaaga agcactcatt caggcttaca
      841 cgccattacg ccttgaaccc aaaggcttaa aatataaagc gatgcgcgag acgatgaagc
      901 aatcaagtat ttctcgtgcc cctgttgatt ttgggcgcct aagggacgtt tttcaaaaac
      961 gccttcaaat actgatggat gataagtcga ttgatttgac gcatacacct gatggtacac
     1021 tggtcgacca ggcatacctt gataaggcaa tggtctttaa cgcgcaaacc acaaccccca
     1081 aagagtatat gaatgcctta ctgggattct gtgttcctca gctgtttgct acaacgcttg
     1141 aatcgccatt cgatactctg gagcatgcag agcgctggtc tgttgccacc caatttttat
     1201 taggccttat caacatccat ggcgtgactc aagggcactt aaatcccgaa accaattggg
     1261 gctggattct ggatgagcat cctgatttaa gccagagcct tgctcaaaca ctggcaaaag
     1321 cacagcaaac aaaggactcg attgagtccg tatgcttagc ctggattaat gcacatgctc
     1381 acgaattaaa acttaacagg tcgtttaacc cccaagacct caaacagatt aaagaagact
     1441 tcgctaccct ctatacacaa attgaagact ccccacattt tgatgaattt ctcgtattcc
     1501 gccgcgaccg aaaaggagat tttgtgacac accaagcctc gatttgtacg agctttgcca
     1561 cgtttgcatg ccatcctcta ttggggttgc ccatcgaggt gactcagcca ctggagcgag
     1621 cgcaagcagc acttggcacc ttgggcacac aaattcccca taatccaatg tctgagaaaa
     1681 aaataaccct tgatgtagcc aagatgtcgc tgcccgaagt tcaggattta tacgagcgaa
     1741 tcgctaccta taaagaccca aaggttaaag caaaacttca cgctcagctc aaacaggagc
     1801 gtcccgactt caagccccaa atcaatgcca aacaattcct gcagtgtgtg gctttcgggc
     1861 aacaaaacga tgcagaagcc ttgctcaaag aagatacgga tagagctcag gaactgcttc
     1921 tggcagacaa catgtcgttt acagactact caggaagaat ctttacttgc actgcttacg
     1981 agtatgctta ttgggctaag gacacccaca tgtgccggat gctcgaaaag tatatggaca
     2041 ataccaccaa acacgacttg ttacaacgag ttcagcgcat cgaagaactc gtaggtgagg
     2101 ggctattcaa agccccaaga gggctgacct acacccaaaa tggggaagaa caccacagcg
     2161 cccattttga tttaacccct ctaaaacagg cattaaagac ttatattgac gcatacgatc
     2221 aaagtcccaa acaaaccgat gctgagtggg aggctttaga cacactctgg gtaaaggttg
     2281 gattgccaca acgggacgtt cctgctcata tcgcacagga atactgtcac cctaatcgtt
     2341 cctttgtgga ggtatccaat aatccatctt tattgggtgc gacgaacccg aataatttga
     2401 tgcgacagct taaattttat aattgggata ctggcgctac tgattcatgg tttactccag
     2461 ggtcttattc ttctaattct ggtttgggtt tttcgtttgc aatattacgc a
//
LOCUS       DATNSN010000263         2510 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.264, whole genome shotgun sequence.
ACCESSION   DATNSN010000263 DATNSN010000000
VERSION     DATNSN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.264"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1682
                     /locus_tag="VAH76_003054"
     CDS             <1..1682
                     /locus_tag="VAH76_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEO1387628.1"
                     /translation="TLAISVLTGLVPFNKAFSGFSNPAVITVGCVMILTYAITQSGVL
                     NNSFIKLKKFSKKTSFQVGLYSGISAFLSAFMNNVGALGIMMPIAIHAFTEIKKSPSI
                     ILMPIAFCSVLGGVITAIGTPPNLLISNFRNQVLGTPYNMFDFTPVGLPVALMGVLFV
                     SLIGWKLVPVRAKYSKSGDSFQISDYMTEVKVPETSSMCEKTVKEFLEMTKANFELIA
                     VAHEGKKKFAYTDDTIIHCNDILIIEASPEDLKNILNSNGLILAGNKPLTSKELYDSD
                     IITMEAVVLPDSSIEGQSASMMRFRSRHKINLLAISREELSFRKKLIDIRFAAGDVVL
                     LQGNAATLRETIVDLGFLPLAERDINVHLTQKKFLPIICFTLSIILASLQILPLDFSF
                     MIAVLALILLKAIPTHSLYRTIDWSILLLLAALIPVGEALQTTGAADLIANGFMKIAG
                     QYSPIFALVAILVITMTLSDLLNNAATALIMAPIAIKIAQSAHVNVDSFLMAVAIGAS
                     CSFLTPIAHQNNTMVMGPGRYRFSDYFRLGLPLELLVVVVTIPTILWVWPL"
     gene            complement(1785..2240)
                     /locus_tag="VAH76_003055"
     CDS             complement(1785..2240)
                     /locus_tag="VAH76_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain protein"
                     /protein_id="HEO1387629.1"
                     /translation="MKQIKGILLSIISLSFLPFLVFASDASISLVGVQGYDLVSYHQK
                     NGPVRGSGNHTAYHNGVAYLFATDENKKTFQANPEKYLPAYGGYCAFAVSVGRKVVSD
                     PLAWKIVDGILYLNLNKQVQEFWSKDIPGNIKKGNIQWEKIKDIDPQKI"
     gene            complement(2288..>2510)
                     /locus_tag="VAH76_003056"
     CDS             complement(2288..>2510)
                     /locus_tag="VAH76_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387630.1"
                     /translation="ALIVLSQKKELNNKGVEIDEHQLHLILKELAKHEGKKFKNNLWF
                     PIGTVDAIRQELEELFISKQTISTAPIMS"
BASE COUNT          757 a          484 c          423 g          846 t
ORIGIN      
        1 ttacactggc catatcagta ttgactggcc ttgttccctt taacaaggca ttttcaggat
       61 tcagtaaccc ggcagtaata acagttggct gtgtcatgat cttaacttat gccatcacac
      121 aatcaggcgt attaaataat tcctttataa aattaaaaaa attttcaaaa aaaacatcat
      181 ttcaagtcgg cctttatagt ggcatcagtg catttctgtc tgcttttatg aataatgtgg
      241 gcgcattagg gataatgatg cctatcgcca ttcatgcttt tacagaaatt aaaaaatcgc
      301 catctatcat cttaatgcct attgcctttt gttcagtttt gggaggagta atcactgcca
      361 taggtacccc tcccaaccta ttaatttcca acttccgcaa tcaagtcctg ggtaccccat
      421 acaatatgtt cgattttact cccgtagggc ttccggtagc attgatgggt gttctctttg
      481 tttcattgat tggatggaaa ttagttccag tgcgggccaa atatagtaaa tcgggagatt
      541 cattccagat ttctgattac atgactgaag taaaagttcc tgaaacctct tctatgtgcg
      601 aaaagactgt taaagagttt ctcgagatga ctaaagcgaa ctttgaactg atagcagttg
      661 cgcatgaagg taaaaaaaaa tttgcataca ctgatgatac gatcattcat tgcaacgata
      721 ttttaattat tgaagcgtct cctgaagatt tgaaaaacat tttgaattca aatggtctaa
      781 tcctcgctgg caacaaacca ttaacatcta aagagctcta cgacagtgat atcatcacta
      841 tggaagctgt tgtattacca gattccagta ttgaaggaca atccgcctcc atgatgagat
      901 ttcgttcacg tcataagatt aatttgttag ctatttcaag agaagaatta tcgtttagaa
      961 aaaaattaat tgatattcgt ttcgcagcag gagatgtggt actacttcag ggaaatgccg
     1021 caacacttcg cgaaacaatt gtcgacctgg ggttcttgcc ccttgcagaa agagatatta
     1081 atgtccattt gacacaaaaa aaatttctac caatcatttg ttttacacta tccatcattt
     1141 tggcatctct gcaaatacta ccactcgact tttcttttat gatagcagtg cttgcgctca
     1201 ttctattaaa agcaatacca acacacagtt tgtatcgtac tattgattgg tcgatactat
     1261 tactattagc tgctcttatc cctgttggag aagctttgca aacgactggt gctgcagatt
     1321 tgattgcaaa tggatttatg aaaattgctg ggcaatattc ccctatcttt gccttggttg
     1381 caattctggt tatcactatg actttatctg atttattaaa caatgcagca acagcattaa
     1441 ttatggctcc cattgcaatc aagattgctc aatcagcgca tgttaatgtg gattctttct
     1501 taatggcagt ggctattggt gcttcgtgtt ccttcctgac tccaattgcc catcaaaaca
     1561 ataccatggt tatgggtcca ggaagatacc gattttctga ttatttccgt ttgggattac
     1621 cattagaact ccttgttgta gtcgttacta ttcctactat tttatgggta tggccattat
     1681 aaaaatagaa attaatatgc attaaacctg taccataatc gataaaacac aatggtacag
     1741 gttagagata tttgaataga tgcaaatggt atattgcaat tacattaaat tttttgtgga
     1801 tcaatatctt taattttttc ccattgaata ttgccttttt ttatgttccc aggaatatct
     1861 tttgaccaaa attcttgtac ctgtttatta agattgagat ataaaatgcc atcaacaatt
     1921 ttccacgcta atggatcact cacgactttc cgccctactg aaactgcaaa agcacaataa
     1981 ccaccatatg ctggcaaata tttttcagga ttagcctgaa acgttttctt gttttcatca
     2041 gtagcaaata ggtaagctac tccattatga taggcagtgt ggttaccact accacggaca
     2101 gggccatttt tctgatgata agaaaccaaa tcatagcctt gaacaccaac cagactaatc
     2161 gacgcatcag aggcaaacac cagaaacggc aagaatgata aagatattat tgataatagt
     2221 attcctttaa tttgtttcat ttttatcgtc cttaattaaa ttgtaactgg tagtacttaa
     2281 atcactatta actcattata ggtgctgtgg atatcgtttg ctttgaaata aacagttctt
     2341 ctaactcttg cctaattgca tcaacagtcc ctataggaaa ccatagatta tttttaaatt
     2401 ttttaccttc atgttttgca agttctttta aaatgagatg caattggtgt tcatcaattt
     2461 ccactccttt gttattaagt tccttttttt gacttaaaac aatcaatgca
//
LOCUS       DATNSN010000264         2494 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.265, whole genome shotgun sequence.
ACCESSION   DATNSN010000264 DATNSN010000000
VERSION     DATNSN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.265"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..543
                     /locus_tag="VAH76_003057"
     CDS             <1..543
                     /locus_tag="VAH76_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEO1387631.1"
                     /translation="LDNITKETQKNEHKEFVKTLVFTREYITNRYSMIIFRLIDDRGN
                     HLIDYDLYLTAGPQYSEQALPAGFFVDRQRNLNNRGKLTYFLDYDIMEGGINTPKMQG
                     NLGFRVKAYPESSDQALAYYRLLDFHSSLADIHKILHPNETVMVEIMLQRRVDRTVFR
                     ISNNLTPAKISGKPTGKKID"
     gene            complement(615..1292)
                     /locus_tag="VAH76_003058"
     CDS             complement(615..1292)
                     /locus_tag="VAH76_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEO1387632.1"
                     /translation="MYSYIDFNDKVHRLIPSKFPPVTLFDWADSAEELEQIALLEGLT
                     NERILAEFGKINLIDQEDWIGGPGSTPIMAAFTHTGFESRFSDGSFGIYYAASSLETA
                     IKETCFHRERFYSASNEKPCSISMREYVANIKKPLIDITDKSYKDLFNPDPACYSKSQ
                     EFGKKICEKKEWGLLYPSVRDLNGLCVAVLRPPALTIPIQGCHLRYIWDGEKISDIYK
                     ESKITNI"
     gene            complement(1294..1668)
                     /locus_tag="VAH76_003059"
     CDS             complement(1294..1668)
                     /locus_tag="VAH76_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HEO1387633.1"
                     /translation="MQTNTRSLQSIPDEVVWKSLTNLVERFDLKESEARILMGDMPRS
                     TYTSHKAKLNRDQKERVSYLLGIYKSLRILFEDGEQARTWINRKNDLPPFKGLTPKEY
                     MLEGGMVRLADVRKFLDFWRGY"
     gene            1881..>2494
                     /locus_tag="VAH76_003060"
     CDS             1881..>2494
                     /locus_tag="VAH76_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEO1387634.1"
                     /translation="MIDTIQSEPKINFTYNYKQPDDYRFSLDSIHLAKFVAKQLESYP
                     DLGSLRVLDLCAGCGVIGIELSWYLQAIRQIDFIEIQDIYTEYFYQNIANVNRPELQF
                     RWHLLNYDELHKKKWEDKFDLIISNPPYFQPGHGMLSPSKFKNRCRFYLDSSFQSYIQ
                     ALGNSLANRGKAYFLLRPLKHHGLDLFSDIQKILQETSVTATKIS"
BASE COUNT          812 a          481 c          405 g          796 t
ORIGIN      
        1 ttagacaata ttactaaaga gactcagaaa aatgagcata aggaatttgt gaaaacactt
       61 gtctttacgc gcgagtatat aaccaatcgt tactctatga ttattttccg gcttattgat
      121 gaccgcggca atcaccttat cgattatgat ctttacctaa ctgccggacc tcaatatagc
      181 gagcaggcgc ttcctgcagg tttttttgta gatcgtcagc gaaatctcaa taatcgaggt
      241 aaactgactt attttcttga ctacgatatt atggaaggtg gaattaatac cccaaaaatg
      301 cagggtaatc ttgggtttcg tgttaaagca tatcctgaat caagtgacca agcccttgcc
      361 tattacaggt tacttgattt tcattcatcg cttgctgaca ttcacaaaat acttcatccg
      421 aatgaaaccg taatggttga aatcatgctt caaagacgag tagataggac agttttccgt
      481 atcagcaaca accttactcc ggcaaaaatc agtggaaagc caactggcaa aaagatagat
      541 tgatacctct ttcgtttgat gcatttgcat cattaatgag aaagtcatct agccacaatt
      601 tcgaaaaaaa tatcttaaat attggtaatt ttactttctt tataaatatc tgaaattttt
      661 tcaccatccc aaatatatct taaatgacat ccttgtatcg gtatagtgag cgcgggaggt
      721 cttaatacag ccacacataa gccatttaaa tctcttacac ttgggtatag caagccccat
      781 tccttctttt cacaaatttt cttgccaaat tcctgacttt tactataaca agcaggatca
      841 ggattaaaca agtctttata acttttatct gtaatatcta ttaatggttt ttttatatta
      901 gccacatact ctctcattga gatggagcaa ggtttctcat ttgatgcgct ataaaaccgt
      961 tctcgatgaa agcaagtttc ctttattgct gtttcaagtg atgaagcagc ataataaata
     1021 ccaaaactgc catcgctaaa tcttgattca aatccagtat gtgtaaaagc agccataatt
     1081 ggagtagatc caggaccacc aatccaatcc tcttgatcta tcaaatttat tttaccgaac
     1141 tctgcaagta tcctttcatt ggttaagcct tcaagtaagg ctatttgttc gagctcttct
     1201 gcagaatctg cccaatcaaa taaagttacg ggagggaatt tagaaggtat tagtctatgc
     1261 actttatcat taaaatctat ataggaatac atcctaataa cctcgccaaa agtccagaaa
     1321 tttccttaca tctgcaagac gaaccatccc cccttccagc atatattctt tgggtgttaa
     1381 acctttaaac ggaggtaagt cgtttttacg attaatccaa gttctggctt gttccccatc
     1441 ttcaaacaat atacgtaaag acttataaat tcctaaaaga taggagactc gttctttttg
     1501 atccctgttt agtttcgctt tgtgggaagt gtacgtagag cgcggcatat cccccatcaa
     1561 aatacgggct tcactttctt ttaaatcaaa acgttctaca agattggtta aagacttcca
     1621 taccacttca tctggtatgc tttgtaaaga ccttgtatta gtttgcatac taaactcctg
     1681 gctgctttct actatattat agtctccaaa gagacaattg tctataaaaa gacatgttaa
     1741 gtgaattaaa tgctcattta atgaaaaata cattgatctg acatgcagaa tatgaaataa
     1801 atttcaaaaa ttaattaaca gtgcaacaag caagggaata aggtgcttta agaccatagt
     1861 tgagcgaaaa taatttcaat atgattgata caatacagag cgaaccaaag ataaatttta
     1921 cttataatta caaacaacct gatgactacc gtttcagtct tgattcaatt cacctggcta
     1981 aatttgttgc aaaacaattg gaatcctatc cagatcttgg ttcattaaga gtattagatc
     2041 tctgtgcagg gtgtggagta attggaattg aactgtcatg gtatcttcaa gcaatccggc
     2101 agattgattt tattgagatc caggatattt atactgaata tttttatcaa aacatagcta
     2161 acgttaatcg acctgagctg caatttcgct ggcatctctt aaattatgat gaactacata
     2221 aaaaaaaatg ggaagacaaa ttcgatttga ttatcagtaa tccaccctat ttccaaccag
     2281 gtcacggaat gctttcccct tcaaaattta aaaatcggtg cagattttat ttagatagct
     2341 cttttcagag ttatattcaa gcgctgggca attcacttgc aaacagaggt aaagcttatt
     2401 ttttattacg tccattaaaa caccatggtc ttgatttatt ttctgatata caaaaaatcc
     2461 tgcaggaaac atcagtaaca gcaacaaaaa tatc
//
LOCUS       DATNSN010000265         2437 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.266, whole genome shotgun sequence.
ACCESSION   DATNSN010000265 DATNSN010000000
VERSION     DATNSN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.266"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..709
                     /locus_tag="VAH76_003061"
     CDS             <1..709
                     /locus_tag="VAH76_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387635.1"
                     /translation="DKLIDIFISNPEKFKKIIHFSSELGQVVDALNPRVANKLMDFIF
                     CNENKIYKHIIRDSYNLCRFLFHRNLRQYSDRLINHILKDPDYFKLVVGDMGNLLRLA
                     INHPQHADTLINMVIKDKEHFKKLISNQSNWSEQLSHFPKYEKIFANNVPIDENEKNR
                     QLYLANAPHAEIRKNARLFAQAERTHSGQFFFSEAMPRELRIIIAGLTRDSYLCNEEE
                     ANQIAQENFSRPMKNSQ"
     gene            complement(880..1578)
                     /locus_tag="VAH76_003062"
     CDS             complement(880..1578)
                     /locus_tag="VAH76_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387636.1"
                     /translation="MKFFNPNDKQESTFDKCMAEYRNLVFGNISERVNQLNERAPNFK
                     PIDFEREQSVQVGVGECAFINADTEERLILGSVGAGTCFILAIVDPNNNSAWLAHVDV
                     LVSMPQLKEMLTKVFTKINPNSAVAHIVGGNDSSIVQGIAICNELEKNGVTISSALLR
                     SKMEMDQLSLAIDPKTGTIYAPEANNLKARNDLWRNLSNRMTKSNEVGSIGLTEISPL
                     PIESTSKHYSAPPI"
     gene            1944..2225
                     /locus_tag="VAH76_003063"
     CDS             1944..2225
                     /locus_tag="VAH76_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HEO1387637.1"
                     /translation="MMTENSSQQRTTEGKNKVAFLALKEDISEALEKGWSITAIWETL
                     HDEGSFTASYNTFRLYIMKYINGQKLGHSLKDPIENHHHRTIKNIDKLL"
BASE COUNT          783 a          435 c          424 g          795 t
ORIGIN      
        1 cgataaactg attgatattt ttattagtaa cccagaaaaa tttaagaaga ttattcattt
       61 ctcatctgag ttgggtcaag ttgtagatgc cctcaatcca cgtgtagcca ataagttaat
      121 ggattttatt ttttgtaatg aaaataagat atacaaacat atcataagag attcatacaa
      181 tctttgtcgc tttcttttcc atagaaattt acgtcaatat tcagataggt taattaatca
      241 tattctcaaa gatcctgact actttaagct agtagttgga gatatgggta atttacttcg
      301 ccttgcaatt aatcatcctc aacatgcgga tacattaatt aatatggtta ttaaggataa
      361 agagcatttt aaaaaactta taagtaatca atctaattgg tctgaacagc tcagtcattt
      421 tcccaagtac gaaaaaatat ttgccaacaa tgtacctatt gatgaaaatg aaaaaaaccg
      481 ccaattgtat ttggcaaatg ctccccatgc agaaattaga aaaaacgcgc gtttatttgc
      541 tcaagcggaa cgcactcata gtggacaatt cttttttagc gaggccatgc ctagagaatt
      601 acgcataata attgctggct taactcgaga ctcctattta tgtaatgaag aggaagctaa
      661 tcaaattgcg caagaaaatt ttagtcgtcc aatgaaaaat tcccaataat tttctatgga
      721 ttccccaact ctggatgaaa acataatggg actagggtca gatcttgccc tttgctccca
      781 ttgtgttgat ttataacttt tgtaattctt gagtatagag gcaaaaggca agatctgacc
      841 ctgaaagctc ttactgaacg ctcttagacc accactgtct caaatagggg gggcactata
      901 atgcttgcta gttgattcga ttggaagtgg tgaaatttct gtaagtccga ttgagcccac
      961 ttcattactt ttagtcattc tattgcttag attacgccat aaatcattac gtgcttttag
     1021 attattagct tctggggcat aaatagtgcc ggttttagga tctatggcta agcttaattg
     1081 atccatttcc atttttgatc ttaataatgc tgagctaatc gttaccccat ttttttctaa
     1141 ttcattacaa attgctatac cttgtactat agatgaatca tttcctccaa ctatatgtgc
     1201 tacagcagaa ttaggattaa tttttgtaaa tacttttgtt aacatctctt tgagttgtgg
     1261 catagaaact aaaacatcta catgagcaag ccatgcagag ttattgttgg gatcaactat
     1321 cgctagaata aaacaagttc ctgcgcctac agagcctaag atcagtcgtt cttctgtatc
     1381 agcgtttata aacgcacatt cgcccacacc aacttgtact gattgttctc tttcaaaatc
     1441 aattggttta aagtttggtg cacgttcatt taattgatta acgcgttcac ttatattacc
     1501 aaatacaaga tttctgtatt ctgccataca tttatcaaaa gtgctttcct gcttatcatt
     1561 agggttgaaa aatttcattg cttacctcgt tgtaagctta ggactgctta tggtttatat
     1621 agttcataag tgagggtttg tctactaact cagatagaca aaaaactgcc aatcaaaaga
     1681 ctcctatgtc cactcaaaga aatgaaaata ttcaagttgc gtactaaagt ggacaattta
     1741 ccttaaaaga aattggtgat tattttggtt tacattattc cagtataagc tagcgtaggc
     1801 tgtttgcttg caaacaggct tgtgtattgg gactagggtc agatcttgcc ctttgctccc
     1861 attgtgttga tttataactt ttgtaattct tgagtataga ggcaaaaggc aagatctgac
     1921 actggttttc cttgatagaa aaaatgatga ctgagaattc aagtcaacaa agaacgaccg
     1981 aggggaagaa taaagtcgct tttctggcat taaaagaaga catatccgaa gccttggaaa
     2041 aaggctggtc gatcaccgcc atatgggaaa cgctacacga tgaagggagt tttaccgcaa
     2101 gttacaatac cttccggtta tatattatga aatatattaa tggtcaaaaa ctagggcatt
     2161 ctcttaaaga ccccattgaa aaccaccatc atagaacgat aaaaaatata gataaactac
     2221 tatgatgatt tggtttaatc aatatgtaca acttgtccca aatgccccaa cttatatttt
     2281 gaggtgggga catttttaag caaatattgg cacatttgtc cccatactat ttgagaacat
     2341 aataaacata ccagtgtaac actcgcttga ttaatcttta acaatttgaa tattggacag
     2401 attagtgtct tcaaagctca tacagttcat atggttc
//
LOCUS       DATNSN010000266         2423 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.267, whole genome shotgun sequence.
ACCESSION   DATNSN010000266 DATNSN010000000
VERSION     DATNSN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.267"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..290
                     /locus_tag="VAH76_003064"
     CDS             <1..290
                     /locus_tag="VAH76_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387638.1"
                     /translation="SQCYDASDEINSNITHCFNEKLKKIPNPLNYKISVHATKTKKSD
                     HGKITVFMINNKGIMLYCIGTAGEKLKINACASDVGKPLTPEQELSIEGFF"
     gene            complement(346..1239)
                     /locus_tag="VAH76_003065"
     CDS             complement(346..1239)
                     /locus_tag="VAH76_003065"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO1387639.1"
                     /translation="MKHFLEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFY
                     ENSTRTRISFELAERHLSMSVVNLDLQTSSETKGEAIEDTIRTLAAMGIQYFVIRHKQ
                     DGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIVEQKKQLDKLKIAILGNIKH
                     SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVIICLRVQR
                     ERLLQDDHLDLDFYRNNFALTQKSLSYAKPDAMVMHPGPMNRGVEIDSEVADGKQSCI
                     LQQVTNGVYARMAILESLIGS"
     gene            complement(1289..1708)
                     /gene="ruvX"
                     /locus_tag="VAH76_003066"
     CDS             complement(1289..1708)
                     /gene="ruvX"
                     /locus_tag="VAH76_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO1387640.1"
                     /translation="MPRGVYLGFDFGYKRIGVAVGQRLTCSASPLSTIEAKAGIPDWN
                     TIQKVITQWNPQALIVGLPTCIDDRELYTTSAARRFAKQLHKRFSLPVHLVDERLSTV
                     EARGYLFEQGGYRQIKKAEVDSIAACVILEQWLQQSE"
     gene            complement(1701..2264)
                     /locus_tag="VAH76_003067"
     CDS             complement(1701..2264)
                     /locus_tag="VAH76_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEO1387641.1"
                     /translation="MAIISSLANHLLIAMPSLKDPNFERSVVYLCEHNEQGSVGLIIN
                     RPLQFPLSIVFEQLQIEPIRVEKNGLPLLFGGPVQPERGFVIHKQMGGWRSSLFLQDE
                     VTVTTSNDIIRAIAYDEGPKDVLITLGYAAWTEQQLEREIMSNTWLICPYKSEILYEV
                     PFEERWEYAGLTLGIKMNQLSSDAGHA"
BASE COUNT          770 a          516 c          407 g          730 t
ORIGIN      
        1 tatcacaatg ctatgatgcg tcagatgaaa ttaattcaaa tattactcat tgctttaatg
       61 aaaaactaaa aaaaatccct aatcctttaa attataaaat tagcgtccat gctacgaaaa
      121 caaaaaaatc tgatcatggg aaaataacag tttttatgat aaataataaa ggaattatgc
      181 tttattgcat cggcactgca ggggaaaaac taaaaataaa tgcctgtgct tctgatgtag
      241 gcaagccact tactcccgaa caagaattgt caattgaggg cttcttttaa ctagccacta
      301 atcagcaaga aacgaaagaa aaattatggc cggatataaa gaaattcaac ttcctatcag
      361 cgattcaagg atagccattc ttgcataaac accgttagta acctgttgta aaatacaaga
      421 ttgcttgcca tcagccactt cactatcaat ttcaaccccg cgattcatag gcccagggtg
      481 cataaccata gcgtcaggtt ttgcataaga cagacttttt tgagtaagtg caaaattgtt
      541 tctataaaaa tccaaatcca aatgatcatc ttgcagcaaa cgttcccttt gaactcgcag
      601 acaaattatc acatccgcac cttccagacc ttcattgaga ttatcagtaa cccgtccaaa
      661 gtgcacttgt gatggttgcc aaatctccgg agaaatcaaa accaactcac caacccctaa
      721 tttactgcaa atgcattgga aagaattagc aacacgtgaa tgtttgatat ttcccaaaat
      781 agcaattttt aatttgtcta attgcttttt ttgttcaaca attgtcacca tatccagtat
      841 cgcttggctt ggatgtgcat gagttccatc accagcattt attatatgaa ctgtatcacc
      901 taacttatta gctaaatttt gctgcagccc atcttgctta tggcgaatta caaaatactg
      961 gatacccata gcggctagcg ttctgatggt gtcttcaatg gcttcgcctt tagtttctga
     1021 tgaggtctga agatccagat tcactacaga cattgacaga tggcgctctg ctaactcaaa
     1081 actgatccgg gttctggtac tgttttcata aaataaatta gcaataatac tttgggaata
     1141 agaagggtat tgttttgtgt gcttaaagta taaagctcgt tgcaataagg attcaatttg
     1201 ttcagatgac aattgactaa tttcaagaaa atgcttcatg ttcccccgcc aaatttcatt
     1261 ttacgccaaa agaaaaatac tctttgagtt attccgattg ttgcaaccac tgctccagta
     1321 taacacaagc tgctatacta tctacctcgg cttttttaat ctggcgatat cccccctgct
     1381 caaacaaata ccctcttgcc tctactgttg aaagtcgttc atcgactaaa tgcacaggta
     1441 aagaaaaccg cttatgcaat tgctttgcaa aacgacgagc agctgatgtt gtatacaatt
     1501 cacggtcatc aatacaggta ggaagaccta caattaaagc ttgtggattc cattgggtta
     1561 tcactttttg aatggtattc caatcaggga ttccggcttt ggcttctata gtagataagg
     1621 gagaggcact acaagttagc cgctgtccta cagcgactcc tatgcgttta tacccgaaat
     1681 caaaacctaa ataaacacct ctaggcatgg ccagcatcag aagatagttg attcattttg
     1741 atgcccagcg ttaatcccgc gtattcccaa cgctcttcaa agggcacttc ataaagaatt
     1801 tcagatttat agggacaaat taaccacgtg ttactcataa tttctctttc caattgctgc
     1861 tctgtccaag cagcataacc taaagtgatt aatacatctt taggtccttc gtcataagct
     1921 atggcacgaa tgatatcatt tgaggtagta accgtcactt catcttgcaa aaacaaactg
     1981 gaacgccacc cgcccatttg tttatgaata acaaatcctc tttctggttg tactggtccg
     2041 ccaaacaata aaggcaaccc atttttttct actcgaatag gttcaatttg caattgctca
     2101 aaaacaatgg atagtggaaa ctgtaaaggt ctgttaataa ttaaaccaac tgacccctgc
     2161 tcattatgct cgcacaaata gaccactgac ctttcaaagt ttggatcttt taaggaaggc
     2221 atggcaatca ataggtgatt ggccagtgaa ctgataattg ccataagaag tccccccaaa
     2281 aattctctcc gctcacttta attatacacc attaatgatt ttaattttat cattttgttt
     2341 ttaatttaat ctattcgtgt tggcatgagc ccacaatacc gaattattat gattttcatg
     2401 acgtaaagat gcttacgaga agc
//
LOCUS       DATNSN010000267         2415 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.268, whole genome shotgun sequence.
ACCESSION   DATNSN010000267 DATNSN010000000
VERSION     DATNSN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.268"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            176..>2415
                     /locus_tag="VAH76_003068"
     CDS             176..>2415
                     /locus_tag="VAH76_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1387642.1"
                     /translation="MDNSFVFNETTLKRVPHFLVLIIILLLGLPYIGLNWGLDFSNLA
                     ITINDGKIVGSQLIESQIRGYFRQTLLQWSGFSLAAVTVLLAFTQYRLSNDKIALIIG
                     LAILFSGSIEALHTLVIDGLALQGPEKNNLDALIWVFSNSVSGLILMTGLSLLVHAGA
                     NKPVRLVTFILLTTFLVLAAIAVIYYAAVIIQIPDMWLYDSYVSRPFELISVFIYLML
                     MIIVYPKIYASYPTILSDSVFYMSLTQVVLAIYIMLLSTTPYDSAFNIAYFLKVVVYF
                     IPCTCFVINYVFSYSAVLEGQKNLKIKQEELKYMASHDPLTNLFNRREFEDLLDKSIA
                     NAQRNNSSLALLLIDLDNFKAINDTFGHFHGDELLKQFANRLVLLVRKGDLLSRVGGD
                     EFTLISLNIESPSSARHLAERILNELISPYTINGKLITVTVSIGISIFPDDGNTTEDL
                     LRKADLAMYKAKNSGKNTYQFYTEQLGYSQHRESELESHLRKALQNDEFELYYQPKFN
                     LINMEIIGAEILLRWNNKALGGVSPNEFIPVAENTGLITELSQWVLQKSCIQIMHWAN
                     QYNKMLSFSINISPVQLTNPQFPKILEKILQEYQYPAEYLELEITENTLMGDQDEISK
                     MLNVISDLGIKLSLDDFGKEYSSLNRLKTLPIDSLKIDKTFISDIHNEHEKVVIIDII
                     IKLANELGMSIVAEGIETIPQLKYLVSRKCHFGQGFLLSKPLTVNEFTSIAYTKLGKT
                     KSNAKV"
BASE COUNT          780 a          435 c          401 g          799 t
ORIGIN      
        1 aattatgctc aaataagaat ccagccccaa atattatttt catatagccc agacttaaaa
       61 tatttctggt aaatacgagg atgcccaggt taacaggata taaaatcagc aattttgcca
      121 ctaacaggca cattttatgc taattttaaa atataagaat tttgagatac aaaaaatgga
      181 taattcattt gtattcaatg aaaccactct aaaacgtgta ccccattttt tggtattgat
      241 tattatttta ctgttaggtc tgccttacat aggtctaaac tggggtttag acttcagtaa
      301 tttagccatt acaataaatg atgggaaaat agtaggctcc caactcattg aatctcaaat
      361 tcgcggttac ttccgacaaa ctctcttaca atggtctgga ttttctcttg ctgcagtgac
      421 ggtattatta gcatttactc aatatcgatt aagtaatgat aaaattgctt taattattgg
      481 ccttgccatt ctattctctg gctccattga agcgctgcat accttggtta ttgatggcct
      541 ggctttgcaa ggtcccgaaa aaaataacct ggatgcttta atttgggttt tttcaaacag
      601 tgttagcggt ttaattctaa tgactgggct ttctttactg gtacatgcag gcgctaataa
      661 accagtgaga ttggtgactt ttattctact gactactttt ttggtcttgg ctgctatcgc
      721 agtcatttat tatgccgctg taataataca aattcctgat atgtggcttt atgattctta
      781 tgtttccaga ccttttgaac tcatatctgt ctttatctat cttatgttga tgattattgt
      841 ttacccaaaa atctatgcat cttacccgac tattttatct gattctgtat tctatatgtc
      901 actcacccaa gtcgtgttgg ctatctacat catgttatta tcaacaactc cgtatgacag
      961 tgcctttaat atagcatatt ttcttaaagt ggttgtttat tttatacctt gcacctgttt
     1021 tgtaattaat tacgtttttt catactcagc ggtactggaa gggcaaaaaa acttaaaaat
     1081 aaaacaggaa gagttaaaat atatggcctc tcatgatcct ctgacaaacc tgtttaatcg
     1141 cagggaattt gaagacttgc ttgataaatc cattgctaat gcccaaagaa ataactcttc
     1201 cctggcttta ctgcttattg atttagataa tttcaaggca ataaatgaca catttggtca
     1261 ttttcatggc gatgaacttt taaaacaatt tgccaatcgc ctggttttac tagttcgaaa
     1321 aggtgatctt ttatccagag tgggtggaga tgaatttaca ttaatctctc tgaatataga
     1381 atccccttcc agcgcacggc atctggcaga acgaatttta aatgaattaa tcagtcccta
     1441 tactatcaat ggcaaattaa tcacagtaac ggtcagtatt ggaatatcca ttttccctga
     1501 tgatggaaat actacagaag atctgttaag aaaagcggac ttggctatgt ataaagcaaa
     1561 aaactcaggg aaaaatacct atcaatttta tactgaacaa ctaggttatt cacagcatag
     1621 ggaatccgag ctggaatctc atcttcgaaa agcattacaa aatgatgaat ttgaacttta
     1681 ttaccaaccc aaatttaatc tgattaatat ggagattatt ggcgcagaaa tccttttacg
     1741 ctggaataat aaagccctgg gaggcgtgtc tcctaatgag tttataccag tagcagaaaa
     1801 cacgggatta ataacagaac tgagtcaatg ggttttacaa aaatcctgca tacaaataat
     1861 gcactgggct aatcaataca acaagatgct ttccttttcc attaatattt cgcctgttca
     1921 attaacaaat ccacaatttc ctaaaattct tgaaaaaata ctacaggaat atcaataccc
     1981 tgcagaatat ctcgagcttg aaattactga aaacacttta atgggggatc aagatgaaat
     2041 ctccaaaatg cttaatgtaa tttctgactt gggaataaag ttgtcattgg atgattttgg
     2101 caaggaatac tcttctctta acagattaaa aactctccct attgacagct taaaaattga
     2161 taaaacattt atctctgata tacacaacga acatgagaaa gtagtcatta tagacattat
     2221 tattaaattg gctaatgagt taggaatgag tattgttgcc gagggtattg aaaccatacc
     2281 tcaattaaaa tatcttgtgt caagaaaatg tcattttggt caaggcttcc tcctgagtaa
     2341 gcccttaact gtaaatgagt tcacttcaat tgcttatacc aagttgggaa aaaccaaatc
     2401 taacgcaaaa gtcat
//
LOCUS       DATNSN010000268         2403 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.269, whole genome shotgun sequence.
ACCESSION   DATNSN010000268 DATNSN010000000
VERSION     DATNSN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.269"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(48..1436)
                     /gene="legK3"
                     /locus_tag="VAH76_003069"
     CDS             complement(48..1436)
                     /gene="legK3"
                     /locus_tag="VAH76_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegK3"
                     /protein_id="HEO1387643.1"
                     /translation="MFDRNIKEIINELQEKDLLPDTGKIIGGPRNQFDKKGEMKQGYL
                     FSVINKEPIGKGGFASVYPVTLYRQGETGFVKKSTDHKKFVTKKFNLNKLKIKYTNLN
                     IENLIEKEFEFCRKAGHLSIKPPVYNNNKCYLTMKRMPGIDLNRIFNKLMANPHYLTE
                     GQRIKITYALLLALKNQVIDKGIIHRDIKPENIHVKVVDPVEVNIFDYGLAKNAGEDD
                     NLFPGSESWAAPEMFTGLKHDSKADIFSMGRVIAFLWGESATNYLSNNPLNKSRNAAK
                     NLNLPAQVKWVEPFLKQMLAERQEDRIEIDSAINQFKQLASINAPKLKLPQSSIKNHS
                     NINNLCFFQNPTKSTNNSDLIQLNIQRNIKKDLQELLENLPSEFSHYRSNIIFKNTIG
                     RFTTIQKMMNELNQIMDENESQDIPMELYDFIHQYQNKFLHASEANIIRKINGFLEDI
                     EYQNLTNSGFEL"
     gene            2024..>2403
                     /locus_tag="VAH76_003070"
     CDS             2024..>2403
                     /locus_tag="VAH76_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2555 family Dot/Icm T4SS effector hydrolase"
                     /protein_id="HEO1387644.1"
                     /translation="MELTMAKELIFIDWDDTLVNRENAEDFESVIDENNTIYQMSPMF
                     IGAVEALKQLQEKYHVVIVSSRGDLNRIRQLQLQSYDLESSIMPLDSFTFEDNDNRLL
                     FTRYGETNFPDKASFILYIQKMSG"
BASE COUNT          766 a          391 c          367 g          879 t
ORIGIN      
        1 atttgttagc gatgcgttcc taacaaaccc agagtaaaat caatatatta taattcaaag
       61 cctgaattgg ttaaattttg atactctata tcttctaaga agccatttat ttttcttata
      121 atattagctt cgcttgcatg caaaaattta ttctgatatt gatgaataaa atcatataat
      181 tccatgggta tatcttgaga ttcgttttca tccatgatct gatttagttc attcatcatt
      241 ttttgtattg ttgtaaaacg gcctattgtg tttttaaaaa ttatattact gcgataatga
      301 gaaaattcac taggaagatt ctctaataat tcctgcaaat cctttttgat attccgctga
      361 atatttaatt gtataagatc agaattgttt gtgcttttag ttggattttg aaaaaagcag
      421 agattattaa tatttgagtg attctttatt gaggattgag gtaatttaag cttcggcgcg
      481 ttaatggacg caagttgctt aaattggttt attgcggaat caatctctat tctatcctct
      541 tgtctttccg ctaacatttg tttcaaaaat ggctctaccc atttaacttg tgcgggcaaa
      601 ttaaggtttt ttgctgcatt tcggctttta ttaagggggt tattgctcaa atagtttgta
      661 gcactttcgc cccataagaa ggcaataact cttcccattg aaaaaatatc agctttacta
      721 tcgtgcttta agccagtaaa catttcaggc gctgcccaac tttcacttcc aggaaatagg
      781 ttatcgtctt cgcctgcatt tttggcaagt ccgtaatcga aaatattcac ttcaacaggg
      841 tcaaccactt taacatgaat attctcaggc tttatatctc gatgaataat tcctttatca
      901 atcacctgat ttttcaaagc caataataaa gcataggtta tcttaattcg ctgaccctct
      961 gtaagataat gaggattagc cattaattta ttgaagatac gatttaaatc tatccctggc
     1021 attcgtttca tcgtcagata acatttatta ttattgtaaa caggtggttt tatacttaag
     1081 tgccctgctt ttcgacaaaa ctcaaactct ttttcaatta aattttctat atttaagttg
     1141 gtgtatttta ttttaagttt atttaaatta aatttctttg ttacaaactt cttatgatca
     1201 gtagattttt taacaaatcc agtttcacct tgccgataca gcgtaacagg ataaacagaa
     1261 gcgaacccac cttttcctat tggttctttg ttaataactg aaaaaagata cccttgtttc
     1321 atctctcctt tcttatcaaa ttgatttcgg ggtccgccga taatttttcc agtgtccgga
     1381 agtaaatcct tttcttgtaa ttcattgatt atttctttta tatttctatc aaacatattt
     1441 taattactcc caaaatagtt attcatgtat ttgctaactc gtctaaattg aaataattag
     1501 acatatttta accattttga tctataaaat aactgtcgaa attgaacagt tatggcactt
     1561 attttttacc tataatcacg ccgacaactt gtttctgttt ctattactta agctacggga
     1621 tttaataaat gaaaattact ggagcaatta agatgatcca aacacatgga cgagcagaca
     1681 gatttgtgaa aaagcaacta ccaaatgatt ggtttttagc actcgaaatt actgattagg
     1741 aagaaatact gataatgatc ttgaacagca aaagattgat accgcattat tgattaattg
     1801 taggtttcgt catagggttg aacctaaaat aatggatgaa aaaaatttaa aaaaccttca
     1861 tttacccctg ggattactct gctcctattc tttttacact tagtttattc ctatttcact
     1921 gctaaaaaat gacagttatt tctattgctc taccgggcta taattgtaaa aaatttaatc
     1981 tttatgatct tgcgattgtt aaattaaatt atttaagatt aatctggagt taaccatggc
     2041 caaagaacta atttttattg attgggatga tacattagtc aatagagaga acgcagaaga
     2101 ttttgaatcg gtaatagacg aaaacaatac tatttatcaa atgtctccta tgtttattgg
     2161 ggcagtagaa gcgctgaaac aacttcaaga aaaatatcat gttgtgatcg tcagttcacg
     2221 aggagactta aataggataa gacagctcca attacaaagt tatgatctag agtcttcaat
     2281 tatgccatta gacagtttca catttgaaga caatgataat cgtttattat ttacaaggta
     2341 tggtgaaaca aactttcctg ataaagcaag tttcattttg tatatccaaa aaatgagcgg
     2401 taa
//
LOCUS       DATNSN010000269         2348 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.270, whole genome shotgun sequence.
ACCESSION   DATNSN010000269 DATNSN010000000
VERSION     DATNSN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.270"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            80..2059
                     /locus_tag="VAH76_003071"
     CDS             80..2059
                     /locus_tag="VAH76_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidJ family T4SS effector polyglutamylation
                     protein"
                     /protein_id="HEO1387645.1"
                     /translation="MAVECTKTSEYPIVDWIKKHHLHFNFIRAFILDYNKKPLTHNQR
                     AFMQQFRDSAAFFFPDQVYLAWLTQSYESGSILNPMYRESRSTHYYHANITDNLLLRT
                     RPKQVNLGPQHFFQKGKGSVKNTYRFNINDGKLMRIQGRTLLFSTNKGNEVIAVKVQK
                     KGEPQSALSNEFQMADYLLKHQRRLNLQSQLPTPLSQYSINRTEIIEKCSKSPDFEKF
                     KNLISDAKSLEIYVYKATPSYFTYLHDKQQSFSQLTSSVQKNVHDLFMLLREGIVFPY
                     LADMFHTHIDESKRSDKGRYQTLVELLSALQSQMGRLDKWQKAVEFVNLRASGIADLG
                     DNLPLTSFLTVSDWTKHYHAELLTGVYHPSFLFLDKSSGSVRSLFNSRRKVFGNYLYL
                     NIIAEYLLVIQLVIGCYGDKVTRKMDSKSKAKVWEHLAELMFSSCAEAVNLITGMPKS
                     RALAFLKQRANVRKHTQQTSFWMTPDYSNLDKLSVQSQQSTLYPGESDYEINSDLIPG
                     VGLSLDGINQDLGDYNQASPLRELEKLLYATVTLIEGTQQLDKQFFQQLDETEKMILS
                     AAGVDKCYQAVAKLLDLARPGCQMQRRLAFTYYEMIKRIYPCSNPADVRFELVAKEEA
                     IIKIQRFWREHKKENQSIEKGFDFDRNTSSSQSPL"
BASE COUNT          759 a          411 c          462 g          716 t
ORIGIN      
        1 aaggtcgaaa agatctgtat cttaatttat ctcaaaaagt ggctgattat cttgaaaaaa
       61 tgaatctaaa atatggagta tggctgttga atgcactaaa accagtgagt atcccatagt
      121 tgactggata aaaaaacacc atcttcattt taattttata agagctttta tactggatta
      181 caataaaaaa ccattgactc ataatcaacg tgcgtttatg cagcagttta gggattccgc
      241 tgcttttttc tttcctgatc aggtttacct tgcttggctt acccaatcct atgaatcagg
      301 ctctatcttg aatcctatgt acagggagag cagatcaacg cattattatc acgcgaacat
      361 tactgataat ctcttgttaa gaacacgccc caaacaggtc aatcttggtc ctcaacattt
      421 tttccaaaaa ggaaaaggct ccgtcaagaa tacttatcgt ttcaatatca atgacgggaa
      481 attaatgcgc atacagggaa gaacattact ttttagcacg aataaaggta atgaagtgat
      541 tgctgtaaag gtgcaaaaaa aaggggaacc acaatctgct ttaagtaatg aatttcaaat
      601 ggcagattat ttattgaaac atcaacgtcg tttgaatttg caaagtcaat tacctacccc
      661 tctcagccaa tattcaatta atcgaacaga gattatcgaa aaatgcagta aatcacctga
      721 ttttgagaag tttaagaatt tgataagtga tgcaaaaagc cttgaaattt atgtttataa
      781 agcaacacct tcttatttta cttatctgca tgataagcag cagagcttta gtcaactcac
      841 ttcatcagtg cagaaaaatg tacatgatct tttcatgctc ctgagagagg gcatagtctt
      901 tccttatctg gcagatatgt ttcacaccca tattgatgag agtaaaagga gtgataaggg
      961 tcgatatcag acgttagttg agcttttaag tgctttgcaa tcacaaatgg gccgtcttga
     1021 taaatggcaa aaagcagtcg aatttgttaa cttacgagcc agcggtatcg ctgatttggg
     1081 tgacaatctt ccgttgacta gcttcttaac tgtttctgac tggacgaaac attatcatgc
     1141 ggaattgcta acgggcgttt accatccatc ctttctattc ctcgataaat ccagtggctc
     1201 agttcgctcc ttgtttaaca gccggcgcaa agtattcggc aattatttgt atctgaatat
     1261 catcgctgag tatctactag ttattcaatt ggtaattggc tgttatggcg ataaagtgac
     1321 ccgtaaaatg gatagtaaaa gcaaagctaa ggtttgggag catttagccg aattgatgtt
     1381 ttccagttgc gctgaagcgg ttaatttgat aactggtatg cccaaatcca gggcacttgc
     1441 ttttttaaag caaagagcca atgtaagaaa acatactcaa caaacgtcgt tttggatgac
     1501 tcctgattat tcaaatctgg ataagctgag tgtgcagtct caacaatcca ctttgtaccc
     1561 cggcgaatca gattatgaaa taaacagtga tttaattccg ggggtaggcc tgagtcttga
     1621 tggcatcaat caggatttag gtgattataa ccaggcaagc cctttaaggg agctagagaa
     1681 attattgtat gctacagtga ccttaataga gggaacccag caactggata agcaattttt
     1741 tcaacagttg gatgaaactg agaaaatgat tttgagtgct gctggagttg ataaatgcta
     1801 tcaagctgtc gcaaaactcc tggatcttgc tcggcctggt tgtcaaatgc agcgaagatt
     1861 ggcttttact tattatgaaa tgatcaaacg aatatatccc tgctcaaatc ctgcagatgt
     1921 gagatttgaa cttgttgcga aagaagaggc gattataaaa attcagcgtt tttggcgaga
     1981 gcataaaaaa gaaaaccaat cgattgaaaa agggtttgat tttgacagga acactagctc
     2041 atcgcagtcg cctttatgaa aaagtcattt caattgtaag agatcatccc cgatcgttag
     2101 aggatatttc aacatgaggg cactgacctg ttatttttaa ttttggagaa ttaaaagtac
     2161 tcataagctt aatgagattt ttcttatttc cgctaacaat tggattttat gctatttaat
     2221 taagacaaga ttttattatt catccaggga attatggtgg aagtttaaac tcaatatcag
     2281 atatgattac gctttgaatt gacaaaaaca agacaaaaaa cgatactttt gcttttataa
     2341 agaaagca
//
LOCUS       DATNSN010000270         2328 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.271, whole genome shotgun sequence.
ACCESSION   DATNSN010000270 DATNSN010000000
VERSION     DATNSN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.271"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            145..2115
                     /locus_tag="VAH76_003072"
     CDS             145..2115
                     /locus_tag="VAH76_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEO1387646.1"
                     /translation="MSRAYYASHIEQFINDSPSYILGELSAFHSFSLDELQRNAWIKQ
                     IEICKTIAPALLSAYIAFEFSIPRMGKRVDVILLHQGIIFVLEFKVGDKHYINSALTQ
                     ALDYALDLKNFHEQSHSRYIVPILVATEAPNFYNNIDRYDDKVYSPLRANKENLLAII
                     NEVTRSISAANLVSEIWMDSTYKPTPTIIEAAQALYKGHSVAEISRSDSGAINLSLTS
                     KAISEIIDCSKLNNRKSICFITGVPGAGKTLAGLNIANERHNIDAGEHAVFLSGNGPL
                     VAVLQEALARNEVAEGKRTNSKISKKEALSKTKAFIQNIHHFRDDALQHFLPPVERVA
                     VFDEAQRAWTLEQTTSFMKRKKGISDFDMSEPEFLISVMDRHRDWSIIICLIGGGQEI
                     NTGEAGLSEWFHAIKKDYSHWDVYVSNKLTDSEYTNGQNIYSDFREGQINIRDELHLA
                     VSMRSFRSEKLSMFVKAILDCQLEDANRILDNLNPLYPIVLTRDLNTAKHWLKSKARG
                     SESMGIIASSGAYRLKPYGIHIKSEIDPIQWFLNPHDDVRSSNFLEDVATEFDIQGLE
                     LDWTCVAWDANLRKTATGWSYKKFSGTKWSNINDETRKRYLLNTYRVLLTRARQGMVI
                     FVPKGDQSDETRLPEFYDSIYNYFSYVGIPTI"
BASE COUNT          751 a          408 c          450 g          719 t
ORIGIN      
        1 gtgtacttct gggtgtactt tcgttttatt aaatttaaaa aatcaaaatt actcattcat
       61 caaatacaga taattaaatg acctataatt catacgagat atgcttttta agcaataaat
      121 aattggaatt gtgaactgaa tacaatgtct cgtgcttact atgcttctca catcgaacaa
      181 tttatcaatg attccccgag ttacattctt ggcgaattat ccgcctttca ttccttttca
      241 cttgatgaac tacagcgtaa tgcttggata aaacagatcg agatctgtaa aacgattgcc
      301 ccagccttat tgagcgccta tattgcattt gaattctcta ttccaaggat gggcaaaaga
      361 gtggatgtta tcctccttca ccagggaatt atctttgttt tagagtttaa agtaggtgat
      421 aaacattaca taaacagtgc gttaacccaa gcacttgatt atgctttaga tttgaaaaat
      481 tttcacgaac aaagccattc tcgctacata gtgccaattc tagtagctac tgaagcaccg
      541 aatttctaca acaatataga tagatatgat gacaaagtat attcaccgct cagggcaaat
      601 aaagaaaatc ttcttgcaat cataaatgaa gttacgaggt cgatatcagc tgctaatctc
      661 gtttcagaaa tatggatgga ctcaacatat aaaccaacac caaccattat tgaagctgca
      721 caggcgctct ataaagggca tagtgttgcg gaaatatccc gttctgattc tggtgcaatt
      781 aatcttagtc tcacttctaa agcaatttca gagataattg attgttccaa gttaaataat
      841 cgtaaatcga tttgttttat taccggggtt cccggtgctg gaaagactct tgctggcctt
      901 aacattgcaa atgaacgaca taatatagat gcaggtgaac atgcggtgtt tttatctggt
      961 aatggtccgt tggtcgctgt gcttcaggag gcgcttgcac gcaatgaagt ggcagaagga
     1021 aaaagaacta atagtaaaat atctaaaaag gaagccttgt ctaaaaccaa ggcatttata
     1081 cagaatattc accattttag agacgatgct cttcagcatt tccttcctcc tgttgagcga
     1141 gttgccgtat ttgatgaggc tcaacgggcc tggaccttag agcaaacaac ttccttcatg
     1201 aaaagaaaaa aaggcatctc agattttgat atgtctgaac cagagtttct aattagtgtt
     1261 atggatcgtc accgagattg gtccattatt atatgcttaa ttggtggtgg ccaagaaatc
     1321 aacactggtg aagcaggact ttctgagtgg tttcatgcga taaaaaaaga ttactcacat
     1381 tgggatgtat atgtttctaa caaacttact gatagtgagt acactaatgg acagaatatt
     1441 tactcagatt ttcgagaggg tcaaatcaac ataagagatg aacttcatct tgcagtttca
     1501 atgagatcct ttcggtcaga gaaactctca atgttcgtga aggcaatttt agattgccaa
     1561 ttagaagatg ccaatcgaat tttggataac ctaaatccac tctatccaat tgttttaaca
     1621 cgagatctca atacagcaaa acattggctt aaaagtaagg caaggggctc agaaagcatg
     1681 ggcatcattg cctcgtcagg agcatataga ttaaaacctt atggaattca tattaagtct
     1741 gagatagatc caattcaatg gtttttaaat cctcatgatg atgtacgttc atctaatttt
     1801 ttggaggacg ttgctaccga attcgatatt caaggcctag agctcgattg gacctgtgtt
     1861 gcttgggatg caaatttaag aaaaacagct actggttgga gttacaaaaa atttagcggg
     1921 acaaaatggt caaacatcaa tgatgaaaca cgcaaaaggt acttattaaa tacctacagg
     1981 gtattgctta ccagggcccg acagggaatg gtaatttttg ttccaaaggg cgatcagagt
     2041 gatgaaacaa ggttgccgga attttatgat tctatttata actattttag ttatgttggt
     2101 atcccaacca tataactctg aagtaaggta aattttctat ttcgacctag gatattctct
     2161 tttagtagtt accttgctct taatcgcata atcctggacc aaagagtgat tcttcagctt
     2221 atcgttgtca attgaaaaag caagagctga taacaatagt aaattaagat atttttgtga
     2281 atatatatgg gtaaagactt gagttttgta ttgggttcta taaaaaat
//
LOCUS       DATNSN010000271         2161 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.272, whole genome shotgun sequence.
ACCESSION   DATNSN010000271 DATNSN010000000
VERSION     DATNSN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.272"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            6..749
                     /locus_tag="VAH76_003073"
     CDS             6..749
                     /locus_tag="VAH76_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO1387647.1"
                     /translation="MLIEHQIKHYRTLYEWFQSPLGVFVAKEFTDQLEPVKKFLKGET
                     LLQLGDCATNPWLNLLDFKHKWIASPFSSTNRIHLECSLNQLPLNRDTMDCVIVPLTL
                     EPFGNNFSLIDEIDRILKPMGFLIFLCINPWSLWGGAMKWGLLNCYGNQTVKLRSAFN
                     LNRIFLQRGYKQCSLTNFCYIPPVNSQSLIKKLTFFDEIGKMIWPFPSGFYCYITQKY
                     EYISPSLTVEPAIESVKKEYDSPLQPATN"
     gene            932..1258
                     /locus_tag="VAH76_003074"
     CDS             932..1258
                     /locus_tag="VAH76_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEO1387648.1"
                     /translation="MTKKSLFKISFANQDEIYEIYARSIKESDMFGFLEVEELVFGEQ
                     TALVVDPSEEKLKMEFCDVKRIFIPVHSIFRIDEVSKQGTSKVKDNLSHGNKVRPFPG
                     AGKIKD"
     gene            1301..1870
                     /locus_tag="VAH76_003075"
     CDS             1301..1870
                     /locus_tag="VAH76_003075"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEO1387649.1"
                     /translation="MTIPDKIKAVYEKSTCLYTSNEVEAALDRMAIKIHETLQDKNPV
                     IICVMVGGLVPLGNLLHRLDFPLEVDYVHATRYRGDLTGGDILWKVRPSSNLAGRTVL
                     VVDDILDGGITLAAIINEIKAMGAAEVYSAVLVDKYRKRVPNGLQKADFVGLQVEDHY
                     IFGYGMDYHEYLRNAPGIFIVHPDHEANK"
     gene            complement(1872..>2161)
                     /locus_tag="VAH76_003076"
     CDS             complement(1872..>2161)
                     /locus_tag="VAH76_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1387650.1"
                     /translation="DFGTGYSCLSYLKELPIDIIKIDKEFIKDLITNQPNQALVKAII
                     AMAKNLGLHLIAEGIENKYQLEILLNLGCHYGQGYYFYHPMPPDKCMQFLQ"
BASE COUNT          723 a          350 c          392 g          696 t
ORIGIN      
        1 acattttgtt gattgaacat caaataaaac attatcgcac tttatacgaa tggttccaat
       61 cacctttagg agtatttgtt gctaaagaat ttacagatca attagaacca gtaaaaaaat
      121 tcttaaaagg agaaacgtta ctacaacttg gagattgtgc tacaaatccc tggttaaacc
      181 ttcttgattt taaacacaaa tggatagcat caccattttc ttctacaaat agaatccatc
      241 ttgagtgttc gctaaatcag ttaccactga atagagatac tatggattgt gttatagtac
      301 ccttaacact agagccattt ggaaacaatt ttagcttaat tgatgaaatt gaccgaatat
      361 tgaagcctat gggatttctc atattcttat gcattaaccc ttggagttta tggggagggg
      421 ctatgaaatg ggggttactt aattgttatg gtaatcaaac ggtaaaattg cgttcagctt
      481 tcaatcttaa cagaattttc cttcaaagag gctacaaaca gtgttcttta actaattttt
      541 gctatatacc accagtaaat agccaatctt taattaaaaa gttaactttt tttgatgaga
      601 ttggcaaaat gatatggcct tttccttctg gcttttattg ctatatcact caaaaatatg
      661 agtacataag cccatcactc acagtcgaac ctgccataga atcagtaaaa aaagaatatg
      721 actcgccctt acagccagca acaaattaga tgaaatttat atttcattaa tattccacat
      781 acgaaagggg ttaaattatt gtcaccatta agtctaaatg aataaatttg gagctaacaa
      841 taagctctag acgactaaac tgctttaaat atttgaagat atacccttac tttttgtata
      901 ctttatcctg cttttagata aggttagata catgacaaaa aaatcgctat tcaaaatcag
      961 ttttgctaat caggacgaaa tttatgaaat ctatgctcgt tcaataaaag aaagcgacat
     1021 gtttggtttt ctggaggttg aggagctggt ttttggagag caaacggcac tggtagttga
     1081 tccgtctgaa gaaaaactta aaatggaatt ttgtgatgtt aagcgcattt ttattcctgt
     1141 acattccata tttcgcattg atgaagtttc aaaacaaggg acatcaaaag tcaaagataa
     1201 cttgagccat ggcaataaag taaggccttt ccctggagca gggaagataa aagattaact
     1261 tcgtttagcc tgtataaaca acttctaagg aaataaataa atgaccattc ctgataaaat
     1321 taaagctgta tatgaaaaat caacctgttt atacacatct aatgaagttg aagcagcact
     1381 ggacaggatg gcaataaaaa ttcatgaaac actacaagat aaaaaccctg taattatttg
     1441 cgtgatggta ggaggtctag ttccattagg taatctattg catcgtttgg attttcctct
     1501 agaggtggat tatgttcatg ctacacgtta ccgaggtgat ttaacgggag gggacattct
     1561 ttggaaagtg agaccatcaa gcaatcttgc aggacgaaca gtcctggttg tagatgacat
     1621 tcttgatggt ggtataacct tggctgccat aattaatgaa atcaaagcca tgggtgctgc
     1681 ggaagtttat agtgctgtat tagttgataa atatcgtaaa cgtgtcccca atggtttgca
     1741 aaaggctgat tttgtcggtt tacaagtaga ggatcattat atttttggct atggtatgga
     1801 ttatcacgag tacttacgta atgcgccagg gatttttatc gttcatccgg atcatgaggc
     1861 aaataaataa attattgtaa aaattgcata catttatcag gtggcatggg atgataaaaa
     1921 tagtatcctt gaccataatg acaacccaag ttaagtaaaa tttcaagttg atatttgttt
     1981 tcaattccct cagcaatcaa atgcaaccca agatttttcg ccattgcaat tattgctttt
     2041 accaaggctt ggttcggttg gttcgtgata agatctttga taaattcttt atcaattttt
     2101 attatatcaa ttggtaattc ttttaaataa cttagacaag aatacccagt gccaaaatca
     2161 t
//
LOCUS       DATNSN010000272         2160 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.273, whole genome shotgun sequence.
ACCESSION   DATNSN010000272 DATNSN010000000
VERSION     DATNSN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.273"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..871)
                     /locus_tag="VAH76_003077"
     CDS             complement(<1..871)
                     /locus_tag="VAH76_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEO1387651.1"
                     /translation="MNFVTFQFVVFLVITVIVFMMMSPYYRKYFILLCSYFFYGTWSV
                     PLISVILFSTSVDYYLGKKIHESHSQTHKKLYLTLSILVNCSILVYFKYMNFFISSID
                     AIGFSLGATQLLLNPMHIILPLGVSFYTFEAISYIVDIYRGEKPASSWLNYNFYIMYF
                     PHLISGPIVRYKELHAQYESKIELPETSRLKEGFQLLLYGFIFKLIIADSMASISDPL
                     FDKYQTIGHLESWLASIAFTIQIYFDFLGYTHIARGVSLFFNIKLPVNFNHPYNARNI
                     SEFWQRWHITLSLW"
     gene            complement(868..2034)
                     /locus_tag="VAH76_003078"
     CDS             complement(868..2034)
                     /locus_tag="VAH76_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO1387652.1"
                     /translation="MSDTHCEKSYRSKLLKQGLLGVLILFLVFLFLESFSRWVAATST
                     APFGESIRNDQKMIVSDSIRQGIVAIGDSIIARSFYPELFSSLLAKNFSKPYEVYNIG
                     VEGSGIIHHRAHLRYLLKRNVKPDFVIMNVSVHNLNLNYFAHDSNEHIIEQRYEDHNS
                     LDYYNRCFTVNKQTLSSTLYCKIAKYSYFFRLVHYYHQQVNNSLQSLLHTQRLRTENL
                     IIKYPAISRQGFSPIFGSLAEDTISTPRMFEFLRHEYRSAFANFKLTTEVLDSIIDDL
                     NNNSIKVILVLAPVYHPVTRKVYDEFNLPSNGAIVSTLENYALKKNIAFINLFEECQD
                     PVYFQDPVHPNIYGAVKITTLLSQKIINKQFVGPDGYLDKELFQYLKSNNENKA"
BASE COUNT          795 a          373 c          348 g          644 t
ORIGIN      
        1 tccataatga caaagtgata tgccaacgct gccagaattc actgatattg cgcgcattat
       61 aaggatgatt aaaattaaca ggtaacttaa tattaaaaaa taatgataca ccacgcgcaa
      121 tatgggtata tccaaggaaa tcaaaataaa tctgtatggt aaatgcaata ctggccaacc
      181 aggattccag atggccaatt gtctgatact tatcaaacaa gggatctgag attgaggcca
      241 ttgaatcggc tatgatcaat ttgaaaataa agccataaag taataactga aacccctctt
      301 ttaaacgcga agtttccggt aattcaattt tactttcata ttgagcatgc agttctttat
      361 aacggacaat cggtcctgaa atcagatgcg ggaaatacat aatataaaaa ttatagttta
      421 accagctact agccggcttt tcccctctat agatgtctac gatataactg atcgcctcaa
      481 aagtgtaaaa agaaacaccg agaggcaaaa taatatgcat aggatttaac aaaagttggg
      541 ttgctcccaa actaaagcca atagcatcaa tactggaaat aaaaaaattc atgtatttga
      601 aataaaccag aatggagcaa ttaaccaaaa tggataaagt caaatagagt tttttatgag
      661 tttgggaatg actctcatgg attttttttc cgagatagta atcaaccgag gttgaaaata
      721 agatgacact gattaatgga acgctccagg taccgtaaaa aaaatagctg caaagcaaaa
      781 taaaatattt ccgataatag ggagacatca tcataaaaac aatgaccgta ataactagaa
      841 aaacaacgaa ctgaaaagtg acaaaattca tgctttgttc tcattattgc tttttaggta
      901 ttgaaacaat tctttatcca aatacccgtc aggccctaca aattgtttgt tgataatttt
      961 ttgcgataac aatgtggtta tctttacagc accatagata ttaggatgta ctggatcctg
     1021 gaaataaaca ggatcctgac attcctcgaa taagtttata aaagcaatat ttttctttaa
     1081 agcataattt tcaagcgtac tgactatagc gccattagat ggcaagttga attcgtcata
     1141 aaccttacga gttactggat ggtaaactgg cgccaagacc aagatcactt taattgaatt
     1201 gttattcaga tcatcaataa tgctgtctaa aacttcagta gttaacttaa aatttgcaaa
     1261 tgcactccta tattcatgtc tcaaaaattc aaacattcta ggcgtgctta ttgtatcctc
     1321 agcaagactg ccaaaaattg gtgaaaaacc ttgtctagag attgctggat atttgattat
     1381 caaattctca gttctcaatc tttgtgtatg cagcaaagat tgcaaactat tgttaacttg
     1441 ttgatggtaa taatgcacta aacgaaaaaa ataagaatac ttcgctatct tgcaataaag
     1501 cgttgatgaa agtgtttgct tatttactgt aaaacaacgg ttataataat ccaaggaatt
     1561 atgatcttca tatctctgct ctatgatatg ttcattacta tcatgggcaa aataattcaa
     1621 attaagatta tggacagaaa cattcataat cacaaaatct ggtttgacat tccttttcaa
     1681 taaatatctt aagtgtgccc gatgatggat aattcctgaa ccttccactc ctatgttata
     1741 cacctcataa ggctttgaaa aatttttagc taataaagaa gaaaaaagtt ccggataaaa
     1801 acttctggca attatggaat ccccaatagc aacaatacct tgcctgatgg agtcactcac
     1861 aatcattttc tgatcattcc tgatactttc accaaaaggt gctgtacttg tagctgctac
     1921 ccatcttgag aaagattcga gaaacaaaaa aaccaggaat aaaatcaata caccaagcaa
     1981 accctgtttg agaagttttg atctgtagct tttttcgcaa tgtgtatcag acataaataa
     2041 cagaaattaa ttttacaata ttcgaatttg cgcagaataa acatatttga taatgatgta
     2101 tagtattaaa agctataaaa aactgaagaa acaatttagt ttaaacgtaa tccgctccct
//
LOCUS       DATNSN010000273         2135 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.274, whole genome shotgun sequence.
ACCESSION   DATNSN010000273 DATNSN010000000
VERSION     DATNSN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.274"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..497
                     /locus_tag="VAH76_003079"
     CDS             <1..497
                     /locus_tag="VAH76_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEO1387653.1"
                     /translation="TAAGATGAQSAGGLSALHSAFKAASQSMGTGDTGSVPGGSSGTK
                     SGGLAQAMGQASKFAGSFGSHLASGAFDVAREKAGSMKASFSAKAAETTGGKIAEAIQ
                     QRASLNAGSSDTDCQSSESQNVTSMGAPEGSFSSGSNDFVPQDDCSDEVSQFVNQKAS
                     GDSQ"
     gene            494..>2135
                     /locus_tag="VAH76_003080"
     CDS             494..>2135
                     /locus_tag="VAH76_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO1387654.1"
                     /translation="MSLIKPNKAIGPQVRKKHQGKMSKQLIILVVLSLLMSMQIGTQY
                     VAYKLHFSDGLGLSWHHVYLPWQSLWWGLKYHHFYPDYFNAAFGLMVMSGCLFLMLVV
                     FAGKHLKKENVSEYLHGSARWANRQDLKDAGLIGNEEGVYVGAFEDEKGEIYYLRHNG
                     PEHILTYAPTRSGKGVGLVIPTLLSWKQSCVITDLKGELWALTAGWRQKNANNKVIRF
                     EPATLKGSARWNPLDEIRVGTEYEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGF
                     ILHAIYKLRNQGEPATFPNIDRMLVDPNTNIADLLIEMTQYHHDDGKTHPVISASARD
                     MIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIKDLMHHDTPVSLYIVTQPN
                     DKARLQPLVRVMLNMVVRLLADKMEFERVDGFIRTKKTYKHRLLCMIDEFPSLGKLDI
                     LQESLAFVAGYGLKFYLICQDINQLKSRERGYGPDETITSNCHIQNAYPPNRIETAEH
                     LSKLTGQTTIVKEHITTSGKRISTFLTQISKTMQEVSRP"
BASE COUNT          593 a          459 c          501 g          582 t
ORIGIN      
        1 ccactgctgc aggtgctaca ggtgcgcaga gtgctggtgg tttatccgca ctacattcag
       61 catttaaagc cgcttcccaa agcatgggta ccggcgatac cggctctgtg ccaggcggca
      121 gctcaggcac aaaatcaggc ggtttagcac aagctatggg gcaggcttcc aagtttgctg
      181 gttcatttgg ttcgcacctg gcctctgggg cttttgatgt ggcgcgagaa aaagcaggca
      241 gcatgaaggc ttctttttct gcaaaagccg ctgaaaccac tggtggcaag attgccgagg
      301 ctattcaaca acgtgcctcg ttaaacgctg gctcatcaga taccgactgt caatcatctg
      361 aatcgcaaaa cgttacttct atgggtgcgc cagaaggcag ttttagttct ggaagtaatg
      421 acttcgttcc tcaagatgat tgttctgatg aagtcagtca gtttgtcaat caaaaggcat
      481 caggagacag tcaatgagtc tgattaagcc caataaagcc attggtccgc aagtaaggaa
      541 aaaacatcag ggcaagatga gtaaacagct aattatttta gtcgttctct cacttctcat
      601 gagcatgcag attggtacac agtatgtggc ttataagctg cattttagtg atggattagg
      661 cctatcttgg catcatgtct atttgccctg gcagtcactc tggtggggct tgaagtacca
      721 ccatttttac cctgattatt ttaatgcggc ttttgggtta atggtgatga gtggctgcct
      781 ttttttaatg ctggtcgttt ttgcaggcaa gcatttaaaa aaagaaaatg tcagtgaata
      841 cctgcatggt tccgccaggt gggctaacag gcaagattta aaagatgccg gacttattgg
      901 caatgaagaa ggcgtgtatg taggggcttt tgaagatgaa aaaggagaga tttattattt
      961 acgccataat ggaccggagc atattttaac ctatgcccca acgcgctctg ggaaaggggt
     1021 gggtcttgtt attcctacct tgttatcctg gaaacaatct tgtgtcatta ccgatttaaa
     1081 aggagagctg tgggcattaa cagctggttg gcgtcaaaaa aatgccaata acaaagtgat
     1141 tcgctttgaa ccagcaacct taaaaggatc agcccgatgg aatcctcttg atgaaatcag
     1201 ggtgggtact gaatatgaag ttggtgatgt acaaaatctc gctacacttg tggttgatcc
     1261 cgatggaaaa gggcttgaaa cccattggca gaaaacatcc caggcattgt tggttggatt
     1321 tattctgcat gccatttata agctgagaaa tcagggagaa ccagccacct ttcctaacat
     1381 tgacaggatg cttgttgatc ccaataccaa tatcgctgat ttactgattg aaatgactca
     1441 gtaccatcat gatgatggca agacacatcc agttatcagt gcttctgccc gcgacatgat
     1501 tgacaggccc gaggaagaag cgggttcagt attatccact ttaaagtcct atctggcctt
     1561 ataccgagat ccagtcgtgg cacacaatgt gtctgcttct gatttctgta tcaaagattt
     1621 gatgcaccat gacacacccg tcagtcttta tatcgtgaca caacccaatg ataaagcacg
     1681 gttgcaaccc ctggttcgtg tcatgctgaa tatggttgtt cgccttttgg ccgataaaat
     1741 ggaatttgag cgggtagatg gatttataag aacgaagaaa acctataaac atcgcctgtt
     1801 gtgcatgatt gatgagtttc caagccttgg caagcttgat attttacaag agtcattggc
     1861 ttttgttgcc ggatacggtt tgaagtttta ccttatttgt caggatatca accagcttaa
     1921 aagtcgagag cgaggctatg gacctgatga aaccattacg tctaattgtc atatccaaaa
     1981 cgcctatccc cccaaccgaa ttgaaaccgc cgaacacctt tcaaaactga caggccagac
     2041 caccattgtc aaagaacata tcaccaccag cggcaagcga atatcaacct ttttaaccca
     2101 gatatctaaa acgatgcagg aagtatcccg tccac
//
LOCUS       DATNSN010000274         2077 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.275, whole genome shotgun sequence.
ACCESSION   DATNSN010000274 DATNSN010000000
VERSION     DATNSN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.275"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1132
                     /locus_tag="VAH76_003081"
     CDS             <1..1132
                     /locus_tag="VAH76_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO1387655.1"
                     /translation="SSTVNYLKQHGFYRILYRHPKLLGQELPQDDILHKLLRSGFIGV
                     GISAVMILLFISTAFFTAPLWLTAITTGLFVGASTYLSGILYGVVNDIFATHANLPYF
                     LLGHQSQQTSLLRTNDKVAQGIAWGVAATFGPVIIATLIFTVAATITAFFVPIATFLL
                     PVMMIAMPLIAVGAEFYARKKAREYLDGEENFYWIGSNDYQRRGLNYMCPTNEERAAW
                     YANSDRNLFGFTKVPLIGLGALIGLVVLSGISMFLPPVLFISPVIAIAIPAAFSAVTC
                     ATLIAGGIYMQVNRNKQLDDRYRLEFERDEIVPDLYLDEDMEYIRKIAKIYAQNEPTV
                     EPKQEVINTENYSFLFDKKPKQAMPNIKQEEFEPARLSQAGA"
     gene            1392..>2077
                     /locus_tag="VAH76_003082"
     CDS             1392..>2077
                     /locus_tag="VAH76_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HEO1387656.1"
                     /translation="MRFNHFIAPLVLLPVCALSQPQNDKGDVFLTKIPTSKNAFVKSY
                     SSESAEFKLLESNARSIDYPTQIIRISGSLESQQLDCDQVHAEILEKVVAPFAPDKFT
                     YNTYISCSYNPDTNMATGFLINSYLDPLTDDAVELLETYLAKNNGSNLLGTPLTFEPA
                     KALVVALTISAGMKKNPNKPPFVEYKQDRSNFYFKSNYEMRNKLFTDIYQNFFTNDPN
                     KILPFLDKWI"
BASE COUNT          654 a          406 c          364 g          653 t
ORIGIN      
        1 cagctctaca gttaattatt taaaacaaca tggtttttat agaatactat accgtcatcc
       61 taaattatta ggtcaagaat tgccacagga cgatatatta cataaattat tgcgctctgg
      121 atttattggt gttggtattt cagccgtaat gattctattg tttatctcca ctgctttttt
      181 tactgccccc ttatggttaa cagctatcac aaccggttta tttgtaggtg cctctaccta
      241 tttaagtgga attctttatg gcgtagtaaa tgatattttt gcaacacatg ctaacttgcc
      301 ctatttcctg ttaggccacc agtctcaaca aaccagtctg ttaagaacta acgataaagt
      361 agcgcaagga atagcatggg gcgtcgctgc aacttttggt ccagtaatta tcgcaaccct
      421 gatatttaca gttgcagcaa caattacggc tttttttgtt ccaatagcta cctttttatt
      481 accagtaatg atgattgcaa tgccattaat tgcagttggc gcagaatttt atgctcgcaa
      541 aaaagcacgt gaatatctgg atggcgagga aaatttttac tggatagggt ctaacgacta
      601 tcagagaaga ggtttaaact acatgtgccc gactaatgaa gagcgagcag catggtatgc
      661 aaactccgat agaaatttat ttggttttac caaggtacct cttattggtc tgggggcgct
      721 gattgggtta gttgtactaa gtggtattag catgtttcta ccaccagtgc tctttatctc
      781 gccagttata gcaatagcga ttccagcagc cttttctgct gttacctgcg ctactttaat
      841 cgctggcggt atctatatgc aagtcaatcg taataaacaa ctggatgatc gttaccgtct
      901 cgagtttgaa cgtgatgaaa ttgtacctga tttatatctg gacgaagaca tggaatatat
      961 tagaaaaatt gcaaaaatat atgctcaaaa tgaaccaacc gttgagccca aacaagaagt
     1021 tatcaacact gaaaattata gtttcctttt tgataaaaaa ccaaaacaag caatgccaaa
     1081 tataaaacag gaagaatttg aaccggcacg actgagtcag gctggagcct aatagtccta
     1141 tatgaacgga ttcaaagaca cgtttagtac tcaatcaatt agtagcgctc tattattgcc
     1201 aaaagagcgc tgcccgaatt aattccaaca aactttgtat atccatgatg tagccaattt
     1261 gcatcattcc tgtatactca aaattagaac ctgttttcat gcgatcactt gtgatcaaaa
     1321 cctgatctaa tctgttccct gctaaactgg ttctttaaat taaaggattt aatttaaaaa
     1381 aggagtttgc tatgagattc aatcatttta ttgctccact ggttttactc cctgtttgtg
     1441 cattaagcca accccaaaat gacaaaggag atgtcttttt aacaaaaatt ccaaccagta
     1501 aaaatgcttt tgtaaaatct tattccagcg aatcggcaga attcaagtta cttgaatcta
     1561 atgccagaag cattgactac cctactcaaa ttattcgtat ctctggttct ctcgaatcac
     1621 aacagctgga ctgcgatcaa gtacacgcag aaattcttga aaaagtggtg gctccttttg
     1681 ctcctgataa atttacttac aatacttata ttagttgtag ttataatccg gataccaata
     1741 tggccactgg ctttctcatc aatagctatt tagatccact aaccgatgat gcagtggaat
     1801 tattagaaac ttacctggca aaaaataatg gatccaatct gttaggtaca ccattgacat
     1861 ttgagccagc aaaagctctt gttgttgctt taactatctc ggcgggaatg aaaaaaaatc
     1921 ctaataaacc accctttgta gaatataagc aagaccgcag caatttctac ttcaaaagca
     1981 attatgaaat gagaaataaa ttgttcactg atatttatca aaatttcttt accaatgatc
     2041 ccaataaaat tctacctttc ctggataagt ggatttt
//
LOCUS       DATNSN010000275         2041 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.276, whole genome shotgun sequence.
ACCESSION   DATNSN010000275 DATNSN010000000
VERSION     DATNSN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.276"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(162..1229)
                     /locus_tag="VAH76_003083"
     CDS             complement(162..1229)
                     /locus_tag="VAH76_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387657.1"
                     /translation="MNLVHQITPTVAQEKVNFVILIPNSDETVLSDFILREEDVPYNE
                     QPSHYQFKKWSAWCSAHFASYYYYIKYKNALLRCKQFLYDFGPLSTLDHAALYDHWVW
                     EMKPYVLNQENICWIGYDYSKSRALCFLSHGTNIELRLLEGDLSDEEFLELGNFFSPI
                     SSSIEILKKNFFELSYWSRYNNCLESACINRNKYKPPSSLWNLRWPIHNILRGSKPDC
                     DIKYIKDLDFDFIPDSQIIYGSEDAIEEIQFVMFPKNGKKNQIAWFRILNKDTFPIKK
                     PTLSRLPSMNSYSGYSNFGLTVLKTKRHELTSNIYIASFSNHYGPHDALWWDSEYAYL
                     LQISAGVNTTIDFFKEIFSNF"
     gene            complement(1301..>2041)
                     /locus_tag="VAH76_003084"
     CDS             complement(1301..>2041)
                     /locus_tag="VAH76_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEO1387658.1"
                     /translation="NDVLAVIDDFLEQLSFEKIWNFIQTEKFKFVHSSRWVNAFSLED
                     GSPLWGNVTISHPRPEACTTEQIYPTNTTIDLFINELIARSSDYSHLIGLKDRDWDFF
                     YARPYLYPRGSGLSWHTDGKYKISGAYVYYCHPNWDINWGAELLINPSPQLYFDYPEV
                     TLISNKKKKVGYHLNSAALNKYADDGIGYYIVPKPNRLVIFKNNILHCIKKVENAAGN
                     HVRASITGFFMSNDKIYETKEAEKEHPI"
BASE COUNT          699 a          362 c          298 g          682 t
ORIGIN      
        1 aaaaaaacag tcttgactcg aactattttt actcgttgga taattaaact ttatatttaa
       61 aatgtaggtc cattaacttt agcaatctgc tatcaaccga tgaatagaaa aaatttaaga
      121 agcgagaacg aagtagtaaa aataattcct atttttctta tctaaaaatt gctaaaaatt
      181 tctttaaaaa aatcaatagt agtattaaca ccagcactta tctgtagtaa ataagcatat
      241 tcactatccc accaaagtgc atcatgtggg ccataatgat tgctaaagct ggcaatgtag
      301 atattgctcg taagctcatg cctctttgtc tttaagacag ttaagccaaa attactatac
      361 ccactatatg aattcatact aggtaatcta gacagtgtag gcttctttat tggaaaagtg
      421 tctttattta aaatgcgaaa ccatgcaatt tggtttttct taccattctt tggaaacatg
      481 acaaattgaa tttcctcaat agcatcctca ctaccatata taatttggct atctggaatg
      541 aagtcaaaat caagatcctt tatatatttg atatcgcaat caggcttact acctcttaga
      601 atattatgga ttggccatct taaattccat aaacttgaag gtggtttata tttatttcta
      661 ttgatacatg cactctccag gcagttgttg taacgtgacc agtaggataa ttcgaaaaaa
      721 ttctttttta agatttcaat tgatgacgat ataggagaaa aaaaattacc cagttctaaa
      781 aactcttcat cactcaaatc cccttcaaga agacgaagtt caatattagt tccatgagat
      841 agaaaacata atgctctcga ttttgaataa tcataaccaa tccaacaaat attctcctga
      901 ttcaatacat aaggcttcat ttcccaaacc caatgatcgt atagagcagc atgatctaat
      961 gttgataaag gcccaaaatc gtacaaaaat tgcttgcatc ttaaaagtgc atttttgtac
     1021 ttaatatagt agtaatatga tgcaaaatgg gccgaacacc aagcagacca ttttttaaat
     1081 tgataatgac ttggttgttc attatacgga acatcctctt ccctgagtat aaaatcagat
     1141 aaaactgtct catctgaatt aggtatcaaa ataacaaaat ttactttttc ttgagctact
     1201 gtaggtgtta tttgatgcac taaattcatt tcataattcc aagcatatcc agattgatag
     1261 ttatcaataa ttttgcagga caaactaatt taaataattg ttagattgga tgttcttttt
     1321 ctgcctcttt tgtttcatat attttgtcat tactcataaa aaaccctgtt atcgatgctc
     1381 tcacatgatt accggctgca ttttctactt tttttataca atgcagaata ttatttttga
     1441 aaataaccaa cctattaggt ttcggtacaa tataataccc aattccatca tcagcatact
     1501 tatttaatgc agcgctatta agatggtacc ctaccttttt cttcttatta ctgattaacg
     1561 tcacttctgg gtaatcaaag taaagttgcg gtgaaggatt aatcagtaat tcagctcccc
     1621 aatttatgtc ccaatttgga tggcagtaat atacataggc acctgaaatt ttgtacttac
     1681 catctgtatg ccaagaaagt cctgagcctc taggatatag ataaggtctg gcatagaaaa
     1741 aatcccaatc tctatccttc aaaccaatta aatgagaata atcagatgat cttgcaatta
     1801 attcatttat aaataaatca atggttgtgt ttgtagggta aatttgctct gttgtgcaag
     1861 cttcaggcct agggtgagaa atagtaacgt tgccccataa aggggaacca tcctcaagac
     1921 taaaagcatt tacccatcta gatgagtgaa caaacttgaa tttttctgtt tgaataaaat
     1981 tccaaatttt ttcaaaagat agttgctcta agaagtcatc aataaccgca agaacatcat
     2041 t
//
LOCUS       DATNSN010000276         2020 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.277, whole genome shotgun sequence.
ACCESSION   DATNSN010000276 DATNSN010000000
VERSION     DATNSN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.277"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            6..>2020
                     /gene="sdcA"
                     /locus_tag="VAH76_003085"
     CDS             6..>2020
                     /gene="sdcA"
                     /locus_tag="VAH76_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdcA"
                     /protein_id="HEO1387659.1"
                     /translation="MVDKIKFKEPERCEYLHIDKDNKVHILLPIVGGDEIGLDNTCET
                     TGELLAFFYGKTHGGTKYSAEHHLNEYKKNLEDDIKAIGVQRKISPNAYEDLLKEKKE
                     RLEQIEKYIDLIKVLKEKFDEQREIDKLRTEGIPQLPSGVKEVIKSSKNAFALRLSPD
                     RPDSFTRFDDPLFSLKRNRSQYEAGGYQRATDGLGARLRSELLPPDKDTPIVFNKKSM
                     KDKIVDSVLAQLDKDFNTKDGDRDQKFEDIKKLVLEEYKKIDSELQVDEDTYHQPLNL
                     DYLENIACTLDDNSTAKDWVYGIIGATTEADYWPKKESESGTEKVSVFYEKQKEIKFE
                     SDTNTMSIKVQYLLAEINFYCKANKLSDANFGEFFDKEPHATEVAKRVKEGLVQGAEI
                     EPIIYNYINSHYAELGLTSQLSSKQREEITEKFTQRYHIIENSPHFDEFFVADPDKKG
                     NIFSHQGRMSCHFLDFFARQTKGKHPLGDLAGHQEALQAGTSNRLHHKNEIVAQGYEK
                     FDQFKKEVVKLLAESKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRNWPAIQKE
                     LEKTTDIPENQKQDLLRLLSRNNLQYDNLSAITWSKYSSKPLLDVELNKIAEGLDLTA
                     KIYNEKRKSEWFKGSRNRARKTQCEELQRVSQEINALLQSESLTKSQV"
BASE COUNT          756 a          352 c          384 g          528 t
ORIGIN      
        1 tgaacatggt tgacaaaata aaattcaaag agccagagcg atgcgaatat ttacatatcg
       61 ataaggataa taaagtccat attcttttac ctattgtagg tggggatgaa ataggactgg
      121 ataatacctg tgaaacaaca ggtgagttat tagccttttt ctatggaaaa acgcatggtg
      181 ggacaaagta ttctgctgaa catcacctaa atgaatataa aaagaacctg gaagatgata
      241 taaaagcaat cggtgttcaa agaaaaattt caccaaacgc ctacgaagat ctattaaagg
      301 agaaaaaaga acgcctggaa caaatcgaaa aatatattga tttaatcaaa gtactcaaag
      361 agaaatttga tgagcaaaga gaaatagaca aactacgcac agaaggaatc ccgcaattac
      421 cttctggagt aaaggaagtc attaaatctt ctaaaaatgc ctttgcttta aggctttccc
      481 cggatagacc agactcattt actcgctttg atgatcctct gtttagtctc aaaagaaaca
      541 gatctcaata tgaagctggg ggataccaac gggcaactga tggattaggg gctcgtttgc
      601 gttctgagct tctaccacca gataaagaca ctcctattgt ttttaataaa aaatcaatga
      661 aggacaaaat cgtagattct gttttagcgc aacttgacaa agattttaat acaaaagatg
      721 gcgatcgtga tcaaaaattt gaggatataa aaaaactcgt tctagaagag tacaagaaaa
      781 ttgattccga acttcaagtg gacgaggata cctatcacca gccacttaac ttggattatt
      841 tggaaaacat agcatgtacg ttagatgaca actccaccgc aaaagattgg gtttatggaa
      901 ttattggcgc tacaacagaa gctgattatt ggccaaaaaa ggaaagtgaa agtgggactg
      961 aaaaagttag tgtattctat gagaagcaaa aggaaataaa atttgaatct gatacaaata
     1021 caatgtcaat taaagtccaa tatctattgg ctgagattaa tttttattgt aaagccaaca
     1081 aattatcaga tgctaacttc ggtgaatttt ttgataaaga gccccatgct actgaagtag
     1141 ccaaaagagt gaaagaagga cttgttcaag gagcagagat tgagcctatt atttacaatt
     1201 acattaacag ccactacgcc gaactgggat taacatctca gttaagcagc aagcaacgag
     1261 aggaaattac tgaaaaattt actcaacgtt atcacattat tgagaactcg cctcactttg
     1321 atgaattttt tgtcgctgat cctgataaaa aagggaatat attctcccat caaggcagaa
     1381 tgagttgtca tttcctggat ttcttcgctc gacaaaccaa aggcaaacat ccccttggtg
     1441 atcttgcagg tcatcaggaa gcactccagg caggaacttc caatcggtta catcacaaga
     1501 atgagatagt cgctcaagga tacgaaaaat ttgatcaatt caagaaagaa gtcgtcaaac
     1561 tgctggcaga gagtaaacca aaagaattat tggattattt ggttgctacc tccccaacag
     1621 gtgttcctaa ttattccatg ctctcaaagg aaactcaaaa ttatattgct tacaaccgta
     1681 actggccagc cattcaaaaa gagcttgaaa aaactactga cataccagag aaccaaaaac
     1741 aagatctttt aagattgctt tctcgtaata atctgcaata cgataatctc agtgcaatta
     1801 cctggtcaaa atattcttcc aagccattat tggatgtgga attaaataaa attgccgaag
     1861 gtttagatct aactgctaaa atttacaatg aaaaaagaaa aagtgaatgg tttaaaggtt
     1921 caagaaatag ggctcgtaag acccaatgcg aagaattgca aagagtatct caagaaatca
     1981 atgctcttct acaaagtgaa tcgttaacaa aaagccaagt
//
LOCUS       DATNSN010000277         1990 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.278, whole genome shotgun sequence.
ACCESSION   DATNSN010000277 DATNSN010000000
VERSION     DATNSN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.278"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..788)
                     /locus_tag="VAH76_003086"
     CDS             complement(<1..788)
                     /locus_tag="VAH76_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387660.1"
                     /translation="MINNSLNLNDLAIFALVVKKRSFTQAALEAGISKAWVSQKISQL
                     EGKLDIKLLNRTTRSLSLTLGGEILFEHCQAMLKEVIHAENHLREYAKAPSGKLVITC
                     PEISGLDLFPALLHQFKLLYPQIKIRLIITDQFVDLTQQGIDFAFRTGKLTDSGLVSR
                     YIGAVPRCLVASPEYLSNNSPIKSPADLFNHQLLKHSSLPDWLLKKEEEAFKIHVKNP
                     VIESNSLIFLHKMSCLHHGIAFLPYYLCSDALKHKTLVKVLPDWL"
     gene            879..1739
                     /locus_tag="VAH76_003087"
     CDS             879..1739
                     /locus_tag="VAH76_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HEO1387661.1"
                     /translation="MRFIIIFIYSLLLNPVFAEVIHYQTDTFAKIAAQYKELGADPKT
                     TLMVFDLDDTLITMTQPLGSVGWWDWQHELQKQGVDSDKLFTKDYQQLVRIQNILFQL
                     VKMEVTDEYVLPFLKDAANQGTTLMGLTARGKEHLSATLMQLKDNQFTIDDKLLFRKK
                     GLKLNNNKTSVAGNFHCPQFSREVVYQQGIMFLDGEDKGEALLCILANTKQDIKTILF
                     VDDAKRNIVSMDKAFANRDEFLVLNVFYTKENARELEIQTNPHLQAQVFEQWNFIKEN
                     LNNVIIQSNF"
BASE COUNT          682 a          350 c          352 g          606 t
ORIGIN      
        1 agccaatcag gtaatacctt aaccaaggtt ttgtgtttca aagcatctga acataaatag
       61 taaggcagaa aggcaatacc atgatgaagg catgacattt tatgcaaaaa aattaaggaa
      121 ttactttcta ttacaggatt tttaacatgt attttaaatg cctcttcctc cttttttaaa
      181 agccaatcag gcaaggagct atgttttagc agctgatgat taaacaaatc tgctggactt
      241 ttgattggtg aattattaga taaatattca ggggaggcga ctaagcatcg tggcactgct
      301 ccaatatagc gggatactaa tcccgagtca gtcaatttcc cggttctaaa ggcaaaatca
      361 atgccctgct gggttaaatc cacaaattga tcggtaataa ttaaacgaat cttgatttga
      421 ggataaagta atttaaactg atgcaataag gcaggaaata agtctaaacc tgaaatctca
      481 ggacaggtaa taaccagctt tccagaaggt gccttagcgt attcccgcaa atgattttca
      541 gcatgaatca cttctttcaa catggcttgg caatgttcaa ataaaatttc accacctaga
      601 gtaagactta aagaacgcgt ggttctgtta agtaatttta tatcaagctt accctccaac
      661 tgagagattt tttgactgac ccatgctttg gaaatcccag cctccaatgc ggcttgagta
      721 aagcttcgct tttttactac taatgcaaaa attgccaaat catttagatt aagtgaattg
      781 tttatcatag cgaacaatga gtttatatta tttggtctaa tcaattaatt ggctaatcta
      841 tatcattaac gcttgatata caataaccag gagctatcat gcgttttata ataattttca
      901 tttattcatt gttacttaac cccgtttttg ctgaagtaat tcattatcag accgatactt
      961 ttgcaaagat tgcggcacag tataaggagt taggagcgga tccgaagacc actttaatgg
     1021 tgtttgattt ggatgacaca ttaattacca tgacgcagcc cttaggcagt gttggttggt
     1081 gggactggca acatgaatta cagaaacaag gagtagattc agataaatta ttcactaaag
     1141 attatcaaca attagtgcgc atccaaaata ttttatttca attggttaaa atggaagtaa
     1201 ccgatgaata tgtactgccc tttctaaaag acgctgcaaa tcaaggcaca accctcatgg
     1261 gattaaccgc gcgtggaaaa gagcatttaa gtgccacgct gatgcaactt aaagataatc
     1321 aattcacgat agatgataaa ttattattca gaaaaaaagg attaaaatta aacaacaata
     1381 aaacatcggt agctggtaat tttcattgcc cacaatttag tcgagaagta gtttatcagc
     1441 aagggattat gtttcttgat ggagaagata aaggtgaagc cctactttgt attctagcta
     1501 atacaaaaca agatattaaa actatccttt tcgtcgatga tgcaaaaagg aatatagtat
     1561 ctatggataa agcctttgca aatcgcgatg agttcctggt tttgaatgta ttctacacca
     1621 aagaaaatgc cagggagcta gaaattcaaa caaacccaca tctgcaagca caagtatttg
     1681 agcaatggaa ttttatcaaa gagaacttaa ataatgtcat tattcagtca aatttctaaa
     1741 acaactaata tttgtagtta attcctgcta taaaaattac aatataatca attgctaatt
     1801 ttggcattga ggggctcata atccgttggt cctaggctca agccctagtg ggcccaccaa
     1861 tttaatttta aaatcaataa gttaaacttg tccttcctaa cccgttgcat agagttcaaa
     1921 tctcaaaagt agcatagttg cttttgataa taattcaaat ctatcaagca aacctccttg
     1981 ctttttagca
//
LOCUS       DATNSN010000278         1985 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.279, whole genome shotgun sequence.
ACCESSION   DATNSN010000278 DATNSN010000000
VERSION     DATNSN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.279"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>1985)
                     /locus_tag="VAH76_003088"
     CDS             complement(<1..>1985)
                     /locus_tag="VAH76_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387662.1"
                     /translation="VIANGLAIFKEKFESGSLFIQRKDNHDSFTHEQILQYYLELALT
                     CGRLDIIKYLIDELHVSLSEEKIFHFLFLHSPYEGNKYSHNIVEIYNYLKNEKEIFSE
                     KALIEEIFLAEAKKGHIQAVKAIIELKLLPNINITDEAGHTAFFYCILESKKKRFSFN
                     PEELGAEDVILSVRDKEILGLIELVNAAILAMEDKNEEKLVKIRKALADSIGRVPETL
                     IEDCLSRAIELSDVKMIQLLLPVCNPDLKFLMLGKVNSVEAGMLLIEHFISTGFVIPE
                     ARSLEGYAHRLGLNEYSAGAEQANRFLLHAVAGFVNRTDNGKDFNTILRALQKTVLLN
                     GIANVEVARKILYQTYLEGEPLLLTVGWTQHGVGLSILWDKESNKTYLVFSNRGQGGI
                     ASCLNAKGLIKKEEPKAGSVIYEIDGKLDEGFFSHFEIGENKLGLFRYGKKAFEKDIR
                     TCLKDARVLPHFIFAAAQNYATCGYVNTKQSIRGLLCVFAMTRGDSLSEELIDRMSRQ
                     YKEFTAHDRNVACSELIEDFQRKIKFDSKHIQKEYQDFIVKLILSHCTSDKPEDIERT
                     QRLFVVLPEEIQEKLAHLIPCHIVARKEDKSSTLQNLSLFKHQEEPSSQNDYFLHRSI
                     GVISDSVDNLLNKYHEMNTLLKFLESHGYTEKDIPTI"
BASE COUNT          608 a          425 c          295 g          657 t
ORIGIN      
        1 aattgtcgga atatcttttt ctgtataacc atgggattct aaaaatttaa gtagtgtatt
       61 catctcatga tatttattca gtaaattatc cacggaatca gagattactc cgatcgacct
      121 atgcaaaaaa tagtcatttt gggaggaagg ttcttcctga tgtttgaaga gactcagatt
      181 ttgaagggtt gatgacttat cttctttccg tgctactata tggcaaggta tcagatgagc
      241 cagtttttcc tgtatctctt ctggtaacac tacaaataat cgttgggttc gctcaatatc
      301 ttctggttta tccgatgtac aatgcgataa aatcaatttg actatgaaat cttgatactc
      361 cttctgaata tgcttggaat caaacttaat tttcctctga aaatcttcaa ttaactcgga
      421 acaagcaaca tttctatcat gagctgtaaa ttctttgtat tgacgactca ttctatcgat
      481 taactcttca cttaaagaat cgcctcttgt catagcaaaa acacataata aaccgcgtat
      541 ggattgtttt gtgttgacgt agccacaagt tgcataattc tgtgctgcag caaaaataaa
      601 gtggggtaat actcgggcat ctttaaggca tgtcctgatg tctttttcaa atgctttctt
      661 cccatatcta aaaagaccca atttattttc ccctatctca aaatgagaga aaaagccctc
      721 gtccaactta ccatctatct catagataac tgaacctgct ttcggctcct ccttcttaat
      781 taaaccctta gcattaagac atgaagcaat tcctccttgc cctcggttag aaaatacaag
      841 ataagtttta tttgattctt tatcccataa tatggataaa ccaacaccat gttgagtcca
      901 tcccaccgta agcaatagag gttcaccttc caaatacgtt tgatataaaa ttttccgcgc
      961 cacctccaca ttcgctatgc cgtttaatag caccgtcttt tgtagcgccc ttaaaatagt
     1021 attgaagtct ttaccattgt ctgtccgatt gacaaatcca gcgactgcat gtaacaaaaa
     1081 tctattcgcc tgttctgcac cagcactgta ttcgttcagc cctaacctgt gcgcataacc
     1141 ctccaaactc cttgcttcgg gtataacaaa tccagtactt ataaaatgct ctattagtag
     1201 cataccggct tcaacggaat tgaccttacc taacatcaag aactttagat caggattaca
     1261 aacaggtaac agaagttgaa tcatcttgac atcactcaac tcaattgctc gcgacaaaca
     1321 atcttctatc aacgtctcag gaactctacc tatagagtca gcgagcgctt tccttatttt
     1381 aaccagtttt tcttcatttt tatcttccat tgccaaaata gctgcattta ccagctcaat
     1441 caatccaaga atttctttat ctcgaactga taaaataaca tcctctgctc ccaattcttc
     1501 aggattaaag gaaaatctct ttttcttact ttccaaaata cagtaaaaaa atgctgtatg
     1561 cccggcttca tcggtaatat tgatattggg taaaagctta agttctatga tggcttttac
     1621 agcctgaata tgaccttttt tagcttcagc caaaaatatc tcttcaatca gcgctttttc
     1681 gctaaaaatt tccttctcat ttttcaaata attataaatt tccacgatgt tatgagaata
     1741 tttattccct tcataaggac tatgcaaaaa tagaaaatga aatatttttt cctcggataa
     1801 ggaaacatga agttcatcaa taaggtattt aatgatatca agacgcccac aagtaagagc
     1861 caattccaaa taatactgaa ggatttgttc atgagtgaaa gagtcatgat tgtcttttct
     1921 ctgtatgaat aaacttccac tctcaaactt ttctttgaat attgctaatc cattggctat
     1981 gactg
//
LOCUS       DATNSN010000279         1968 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.280, whole genome shotgun sequence.
ACCESSION   DATNSN010000279 DATNSN010000000
VERSION     DATNSN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.280"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            220..381
                     /locus_tag="VAH76_003089"
     CDS             220..381
                     /locus_tag="VAH76_003089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387663.1"
                     /translation="MFVLHFNKEFASLIDLVLKAMGLGIRSINKIIENARNAKKKNEK
                     KQRAKRKIY"
     gene            690..1553
                     /locus_tag="VAH76_003090"
     CDS             690..1553
                     /locus_tag="VAH76_003090"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019997.1,HMM:NF037521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HEO1387664.1"
                     /translation="MKSYQEFCALNARRLFAIIEKHGGLLQWKKEWSGEGLNALPQGI
                     NGIYRGGNLFSLFFAQMENGFKSNQWLTFNQIKKGGGQVLNGSKAEEIYFWSLKDQIE
                     LNQKTNESEKKSSFIFKTYYVFNLEQTTLFKKEGGQNEQFQCNQLLNYIKPSISHFGN
                     HAFYTPVNDTIVLPHREQFTDKAAYEATLLHELTHWTGVEYRLNRESLKAYGTEKGRA
                     EEELVAEFGAFFLCAFFKIKSDIENHASYVNSWKTLLNEKEIMRATNMAAKAFHYLVE
                     PLQKEQAQIAA"
BASE COUNT          689 a          300 c          372 g          607 t
ORIGIN      
        1 ctttgaataa tctacttgta aaaagacact ctaacaccat tttaaatggt aatcttcgtc
       61 atctatcctc ctattcgcta tgaatattga ctacataaaa aaacaataca aggtctactc
      121 gtcattttac tactgtgttc caccttgtat tgtcttttta ttccattaaa acaataaatt
      181 gcgaatgggg gatggatgac gaagcaacaa cagtactaaa tgtttgtttt gcattttaac
      241 aaggaattcg cttcgctcat tgatttggtt ttgaaagcga tgggtttagg aatcagaagc
      301 atcaacaaaa taatagaaaa cgcaagaaat gcaaagaaaa aaaatgagaa aaaacaacgt
      361 gcaaaaagaa aaatttattg aaaaatcata atcttagcgt taaaataaag gggtatttaa
      421 gtcgtgatat caagctgttg gagcagctca atatcactca tcatcaacac tattggagta
      481 gtgaaaatga ctaatgtgaa tctatcaaaa cctttccatt gtatcaaatc ttttttatcg
      541 ctctaaaaat aaacaagtct atttcaaatc atttaaaact gcttatatcc agaaatcagc
      601 tggattccat cgtctacagt aacctttaac gcattgctca atttagccaa ggacacgtta
      661 aagaacaaat agataaagga tgagatatca tgaaatcata tcaggaattt tgtgcactca
      721 atgctcgccg tttgtttgca attattgaga aacatggggg tttattgcaa tggaaaaaag
      781 agtggagtgg tgagggatta aatgcgttac ctcaaggcat caatggtatt tatcggggtg
      841 gcaacttgtt ttctttattt tttgcgcaaa tggaaaatgg gtttaaatcc aaccaatggc
      901 tgacgttcaa ccaaattaaa aagggaggtg gacaagttct taatggatca aaagcagaag
      961 aaatttattt ttggtcttta aaggatcaga tagaacttaa tcaaaaaact aatgagtctg
     1021 aaaaaaaatc atcctttatt tttaaaacct attatgtttt taatctggaa caaactacct
     1081 tgtttaaaaa ggaggggggt caaaacgagc agttccaatg caatcagttg ctgaattata
     1141 ttaagccatc tattagccac tttgggaatc atgcttttta tacaccagta aatgatacta
     1201 ttgtgttgcc tcacagagag caattcactg ataaggctgc ctatgaggcg actctattac
     1261 atgaattaac gcactggact ggtgtggaat atcgcttaaa tagagaaagt ttgaaagcct
     1321 atggaactga aaagggtagg gcggaagaag agttagttgc tgaatttggc gcatttttcc
     1381 tttgtgcatt ttttaaaatt aaaagtgata ttgaaaatca tgcctcctat gttaactcat
     1441 ggaaaactct attaaatgaa aaggaaatta tgcgagctac taatatggca gcaaaagcat
     1501 ttcattatct tgttgagcct ttgcaaaaag agcaggctca aattgcagcg tagtctagtg
     1561 ccccgaagag gggcttcaaa attgagacct ggttgagcaa aagatcttga ttttgtgttg
     1621 gttaaagctt ggcttctgtt gttattgaat aatatatttt taagttcgaa aggggattaa
     1681 ggataaaaaa ataactctct tttaatactt aagtgggatc tattttaact ctaattctca
     1741 tgctttttta ttgcgctttg ctaaaagaac aagcatctct ctaagtactg gattttagag
     1801 tataagccca gtgtattcac agacttatcc acagaaaatg gggataagta atcaatgcaa
     1861 aatattttga taagtcctta caggacaagg ggtttaataa aaaaattatg ctatcaattt
     1921 gtaataaggc tgaaaaatgg gtgtggttat ccacagagga aaatatgc
//
LOCUS       DATNSN010000280         1917 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.281, whole genome shotgun sequence.
ACCESSION   DATNSN010000280 DATNSN010000000
VERSION     DATNSN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.281"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..386)
                     /locus_tag="VAH76_003091"
     CDS             complement(<1..386)
                     /locus_tag="VAH76_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387665.1"
                     /translation="MYLVKLGMFMVSILLAFTLRADPGVDIIQSCKNMKAAKNTIVMK
                     ELDGFGFNEKNEANCQNQFDSKIEGAIYGAVVCDDKSYFIINNKKISTESAKNLSMNE
                     SVKPNIPIHYTSMWWKIEDGDKSWLCV"
     gene            complement(377..1324)
                     /locus_tag="VAH76_003092"
     CDS             complement(377..1324)
                     /locus_tag="VAH76_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387666.1"
                     /translation="MPKAYSEITSPHKVFNYPVEAIYLIVVPNSNGGISWGYSYEGAC
                     KMLGDDYFWGIVPSKSVSPACYTLPSLNMIIGTDSWFCADGSISYFNTPAHFCSDTFE
                     CPDGSWVLSTDKSICEREDLPCIPDPQNVSEEQLLAAIAYGESHWSNIYEEMAAIASA
                     TLRSKEAKHYKTINELVKKSPNFSYVTSHPDDPKRRNERYYNVMCSIDSKGVDLAYKA
                     AQNALNHGIDYSNGACFWDGLDLKAKGTQAYRYLQGFKISQKEHNVLGVDEPPLLNKK
                     GAKGKIYNFTYVSTAGIGKSIFWKLSDDFLVAWGGKQCI"
     gene            complement(1763..>1917)
                     /locus_tag="VAH76_003093"
     CDS             complement(1763..>1917)
                     /locus_tag="VAH76_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEO1387667.1"
                     /translation="LKNCDHLLSGDWRGFRECHISPDWLLIYKNENELKLLRLVRTGS
                     HSDLFR"
BASE COUNT          638 a          363 c          273 g          643 t
ORIGIN      
        1 acacataacc aggatttatc accatcttca attttccacc acatggacgt gtaatgtatt
       61 ggaatatttg gcttcacgct ttcgttcata gataaattct ttgcagattc cgtactgatt
      121 tttttattgt taataataaa gtaagattta tcatcacaaa caactgcccc ataaatagca
      181 ccttctattt tactatcaaa ttggttctga caattagcct cgtttttctc attgaatcca
      241 aatccgtcaa gctctttcat aacaatagta ttttttgcag ctttcatatt tttacaagat
      301 tgaataatgt ccacccctgg atctgctctt aaagtaaacg ccaataatat gcttaccatg
      361 aacattccta attttactaa atacattgct ttcctcccca tgcgacaaga aagtcgtcgc
      421 ttaatttcca aaaaatagat tttcctatcc ccgcggttga tacataagta aagttataaa
      481 tctttccttt cgctcctttt ttattgagta acggtggttc atcaactcct aaaacattat
      541 gttctttttg agaaatttta aatccttgaa gatatctata tgcttgagta cctttagcct
      601 ttaagtctaa gccatcccag aaacatgctc cattagaata gtcaattcca tgatttagag
      661 cattttgagc cgccttatat gctaaatcaa cccctttcga atctattgag cacattacat
      721 tataatatcg ttcatttcgt cttttggggt catcgggatg tgaagttaca taagaaaaat
      781 ttggactttt ctttactaat tcgttaattg ttttgtaatg ttttgcctct ttgcttctta
      841 aggtggcact cgcaatagca gccatttcct cataaatatt ggaccaatgt gattcaccgt
      901 aagctattgc tgctaataat tgctcttctg aaacgttttg tggatcagga atacaaggca
      961 aatcttctcg ttcacatatt gatttatctg tggataatac ccaactccca tcaggacact
     1021 caaaggtatc gctacaaaaa tgagcaggag tattaaaata gctgatactg ccatcggcac
     1081 aaaaccagct atcggtgcca ataatcatgt ttaaagatgg tagagtgtaa caagctggag
     1141 atacgctttt agaaggcaca attccccaaa agtaatcatc ccctagcatt ttgcaggcac
     1201 cttcataaga ataaccccat gaaatgccgc cattagaatt aggaacaaca attagataga
     1261 ttgcctcgac aggataattg aatactttat gtggtgaagt aatttcacta tatgcttttg
     1321 gtatagcaag tgcattaaaa aaaagaatta acaaacttcc taatttcaaa caacaacatc
     1381 ctattttgac tattcaagaa aatgattatt taaaatcatt tattccataa ttagcgagat
     1441 gtcggaatta taaataataa atttttaatt accagaattc attttgattt tttaaagcta
     1501 tttaatatct acttaaggcc taaaattact acattgcaaa gcaatgtata ttgcgccctt
     1561 agaaaattct tttcttcggg ttaatagaag aataaatatg agagtatgat ttagaaaatt
     1621 ttcatttaga agcttataag ataatttgat aatttcagta gtcaagtaat ccttgactac
     1681 tcaatagtga atataaaaat taatctatcc aaaacgaaca atccggaata tccgaagact
     1741 tcattcaaat taaaattatc cgttaacgaa ataaatcaga atgagaccca gttcgaacta
     1801 atcttaatag ctttagttca ttttcatttt tataaataag caaccaatcc ggtgaaatgt
     1861 gacattctct aaacccccgc caatcccctg aaagaagatg atcacaattt ttaagag
//
LOCUS       DATNSN010000281         1886 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.282, whole genome shotgun sequence.
ACCESSION   DATNSN010000281 DATNSN010000000
VERSION     DATNSN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.282"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..795
                     /locus_tag="VAH76_003094"
     CDS             <1..795
                     /locus_tag="VAH76_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO1387668.1"
                     /translation="QIIISDNASSDNSLALLEQSELNKQTITIHRNNSNYGFSYANNA
                     VFTKISTPFILYLNPDCFVENDTIENLLHTMHQHNDAGMVGCLVNNMDGSVQIGCCGL
                     TPTPARVLNQLLKLSKIFPDNPKFWGYLFSKEKLPTKPTEVELISGSCMFVRKKAIDE
                     VGLLDDNYFLYCEDYDWFYRFTQSDWKIIFNPNTKVTHIKSFSTKQIPFKVLLYKAKG
                     MWRFYNKFFKQHSSLTTTLIIRVGIIARFFSLSGLLLFKKLLRTFN"
     gene            complement(908..>1886)
                     /locus_tag="VAH76_003095"
     CDS             complement(908..>1886)
                     /locus_tag="VAH76_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1387669.1"
                     /translation="ISGVISFGKQLPFQSLAVNAFFWLSGFLITYHCITKKPYSFVEY
                     MIDRFCRIYVIYIPVLILSVFLLVEAELASMPKLKEWIANILMIQHTPFNRIFEFLPT
                     IPPLAQISPLWSIAVEWWLYTLFGIAFFFHKSSFATRLIMSLLIIPALLVAGYFTLKE
                     YVALVWFLGSGCAYHFCNINRKYNNHAILMLSLITGAAFLVRFYVLKHSLMNMYDLQL
                     VIPGCIFLYSLLLLLSTNKQNKKIELISTFLAFISYTLYLSHEPIRRVVSTFIEPTNL
                     KRGFLICGVCIACATIIAYLLENKHLVVREWLKNKLLQKNTSNKLAYST"
BASE COUNT          677 a          330 c          294 g          585 t
ORIGIN      
        1 caaattatta tttcagataa tgcctcttct gataatagtt tagcgttatt agaacaatct
       61 gaattaaata aacaaactat tactattcac agaaataatt ccaactatgg attttcttac
      121 gccaataatg cagtttttac caaaatctca accccattta tactctatct taacccagat
      181 tgtttcgttg aaaacgatac cattgaaaat cttttgcata caatgcacca acacaatgat
      241 gctggaatgg ttggatgcct ggtaaataac atggatggta gcgtgcaaat tggctgttgt
      301 gggcttactc caacccctgc cagggtttta aatcaattat tgaaactatc aaaaatcttc
      361 ccggataacc ctaaattttg gggatactta ttttcaaaag aaaagctccc gacaaaacca
      421 actgaagtag agttgatttc agggtcttgt atgtttgtac gcaaaaaagc aattgatgaa
      481 gttggccttc tggatgataa ctatttccta tattgtgagg actacgattg gttttatcga
      541 tttacacaaa gtgattggaa aattattttt aatccaaata caaaagtcac tcatataaaa
      601 agtttctcca ccaaacaaat tccatttaaa gtactgttgt ataaagcaaa gggcatgtgg
      661 aggttttata ataaattctt taaacaacat tcttctctaa ctaccactct aattattaga
      721 gttggaatta tcgcgagatt tttttcttta agcggtttgc ttctttttaa gaaactgttg
      781 cgtactttca actagaaaat tcaccgcctt taattctact ctatcaccta tttaacagat
      841 ttactcaata gacaatcaag gtctattgag atctgctcta ttccgacaaa aatatcggat
      901 tatttagtta tgttgaataa gccaacttgt ttgatgtatt tttttgcaaa agtttatttt
      961 ttagccactc gcgaactacg agatgtttgt tctctaataa ataagcgatg atagttgcgc
     1021 aagcaataca aactccacaa ataaggaagc ccctctttaa attcgtcggt tcaataaatg
     1081 tggatacaac tcttcttatt ggctcatggc tcaaatacag tgtataggaa ataaatgcaa
     1141 gaaatgttga aattaattca atttttttgt tttgtttgtt tgttgacagt aataacaaca
     1201 gtgaatacaa aaagatacag ccaggaatga ctaattgcaa atcatacata ttcattagtg
     1261 aatgctttaa gacatagaat ctaaccagaa aagctgcgcc tgtgattaaa ctaagcatta
     1321 aaatcgcgtg attattatac ttcctattta tattacagaa atgataggcg cacccagaac
     1381 ccagaaacca taccaaagca acatactctt ttaatgtgaa atatccagca actagcaaag
     1441 caggaataat taataagctc attataagcc tggtagcaaa agagctttta tgaaaaaaaa
     1501 aggctatccc aaacaaagta tataaccacc actcaactgc tattgaccac aatggggaaa
     1561 tttgagctaa aggtggaata gtaggtaaaa attcaaaaat tcgattaaaa ggagtatgtt
     1621 gtatcatgag aatattagca atccactctt taagtttagg cattgatgcc aactctgcct
     1681 ccactaacaa aaaaactgat aaaatcaaca caggaatgta aatgacataa attctgcaga
     1741 aacgatcaat catatattct acaaatgaat acggtttctt tgttatacaa tgatacgtaa
     1801 tcaaaaatcc agagagccaa aaaaaagcat ttactgccaa ggattggaaa ggcaattgtt
     1861 ttccaaatga aattaccccg gatatg
//
LOCUS       DATNSN010000282         1880 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.283, whole genome shotgun sequence.
ACCESSION   DATNSN010000282 DATNSN010000000
VERSION     DATNSN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.283"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..376
                     /locus_tag="VAH76_003096"
     CDS             <1..376
                     /locus_tag="VAH76_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387670.1"
                     /translation="EKIDFKLNIPKGLEYSDIYKLYIIDGVHKEWPVEWPTVSTVKKK
                     IKKIQIEQFEIKGDLQTIIVTSYLLYKQFYLKVEHKIPLDSYTTGCWFKKKKDLEEAI
                     KKIRLKQERNLVDGRIDRITFS"
     gene            644..898
                     /locus_tag="VAH76_003097"
     CDS             644..898
                     /locus_tag="VAH76_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387671.1"
                     /translation="MDSNFQVINMDEYNEFLDICKSNGFDPDDFKFVEHDISLPRPNS
                     VGSITGKVTISKNTKKKTYNTGDGSSWPLQFENDLKQRLF"
     gene            950..1585
                     /locus_tag="VAH76_003098"
     CDS             950..1585
                     /locus_tag="VAH76_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387672.1"
                     /translation="MDNNCRKLLKSSIKGLDERLKHDSLDEERWIVKSFVEANNIIFD
                     NNKLISPPQDSKIDQIYEDCHFQIKKILYPDDYKPKLEDKKYRKKLEEVEDMKDFLDA
                     TVLETNFNGKYQKLDYLEQKIAKEALMWAKSGRYPPPIRSKLDLLFYLEVDDIKSENR
                     LQGIVFDKDKFSDLMESGWRSISYLFGALSLVLYVSDEAPSFLKNIYNSQS"
BASE COUNT          706 a          250 c          346 g          578 t
ORIGIN      
        1 agaaaagatt gattttaaat taaacattcc taaaggactt gaatatagcg atatatataa
       61 attatatatt atcgatgggg tacataaaga atggccagta gagtggccaa ctgtttcaac
      121 tgtgaagaaa aaaatcaaaa aaatccagat tgagcaattt gaaataaagg gagatttaca
      181 gactatcatt gtgacatcct atctgctgta taaacaattc tatttaaaag tggaacataa
      241 aattccttta gatagctata caaccggatg ttggtttaag aaaaagaaag atcttgagga
      301 ggcaattaaa aaaatacgtc tcaagcaaga aagaaattta gtagatggca ggattgatag
      361 aatcacattt tcataaaata agagagtttt gatattgctt taggtttatc taattggttt
      421 ttggaaaaac tatgttgtgt ccctgaaaaa atgttttgcg cattatatcg attaataaaa
      481 tctggtgttt atgaagtcga tataagaatt tataaacggt aaaggtcaac tatttgatcc
      541 gcctactaat ttgtaaatta atagacattt taaggctctg taaaagtcat tgtatttttg
      601 gactatagtt aaagacatat ttgagcaaaa caaggacgta gttatggata gtaattttca
      661 ggttataaac atggatgaat acaacgaatt tcttgatatt tgcaagtcaa atggctttga
      721 cccagatgat tttaaatttg tggagcatga tatttctttg ccgagaccta attctgttgg
      781 ctctattaca gggaaagtaa ccatttccaa aaatacaaaa aagaaaactt ataatactgg
      841 agatggaagt agctggccat tacagtttga aaatgattta aaacaaagac tattttagag
      901 tcgttcctga taatattatt aacgctttgt ctaactggag tagaaatgta tggataataa
      961 ttgtaggaag ttattaaagt cttctattaa aggtctagat gaaaggttaa agcatgactc
     1021 attagatgaa gaaaggtgga ttgtaaaaag ttttgtggaa gctaataata tcatttttga
     1081 taataataag ttaattagcc cgccccaaga tagcaaaata gatcaaattt atgaagattg
     1141 tcatttccaa ataaaaaaaa ttctttatcc agatgactat aagccaaaac tggaagataa
     1201 aaagtatagg aaaaaactgg aagaagtaga agatatgaaa gactttcttg acgcaacagt
     1261 actagagacc aatttcaatg gaaaatatca gaagctagac tatctagaac aaaagatagc
     1321 caaagaagcc ttaatgtggg cgaaaagtgg aaggtacccg ccaccaatca gaagtaagtt
     1381 agatctttta ttttatcttg aagtagatga catcaaatct gaaaatcgtc ttcaggggat
     1441 agtgttcgat aaggataaat ttagtgattt aatggagtca ggatggcgat cgatttcata
     1501 tctttttgga gcattatcgt tagttttata tgtttccgat gaagccccat cttttttaaa
     1561 gaatatatat aatagtcaaa gttaaatatc cccttgtatt caaataatga acttgaaaaa
     1621 attaaccaca tttaactagc ttcttgttga tttcccatca gacaagagag tagggcagta
     1681 gaggcaggaa aacccccgat tttcttcctg gtaccctagt caaaaaggga agtgtcccaa
     1741 aaacccaagt tttatggcta ttaaatcgat atatagataa tctgttttct cgaaacaacg
     1801 atttaataat cataactaaa taatcaatat ggttgtttcc ttattcacaa atgatcagca
     1861 agcgtaggtc aggccaaggt
//
LOCUS       DATNSN010000283         1858 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.284, whole genome shotgun sequence.
ACCESSION   DATNSN010000283 DATNSN010000000
VERSION     DATNSN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.284"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(72..1124)
                     /locus_tag="VAH76_003099"
     CDS             complement(72..1124)
                     /locus_tag="VAH76_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEO1387673.1"
                     /translation="MHHLKLAERLGIQFSIIQAPMAGGATTPELVAAVSNSGGLGSLG
                     AGYMRPDEIRQAIIKIRQLTSKPFAVNLFIPEAHHATPEQIQSACDDINLCCTELNIE
                     ISPVSKPYSLPFVDQMQILIEEKIPVFSYAFGTLEPMWIKQLKKNGTFLIGTATTIHE
                     AIILEASGIDAIVSQGSEAGGHRGTFIGDAEEALIQLSELIPQLVETIRVPVIAAGGI
                     MNGKGIISAINSGASGVQMGTAFLSCFEAGISYKYKQTLLSQQQDNTVLTRAFSGKLA
                     RGIRNKFITCMDNRKINILDYPIQNALTQVMRQKARENDNIDFMSLWAGQSAHLCRSM
                     SASDLISTLVIEAEMK"
     gene            complement(1102..1713)
                     /locus_tag="VAH76_003100"
     CDS             complement(1102..1713)
                     /locus_tag="VAH76_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO1387674.1"
                     /translation="MKKIAPTLKNEIINTAIDIAKDTSWEKVRLIDIAKALNIDLNTI
                     RYFFAEKEQIIDGCFDRADEAMLNACSRPKFDLLTAKEKLHDLLMTWLSTFEGQQKVI
                     RQMIFHKLEPGHLHLQLPAIKRISQTVQWWREAAGLRETFIQRAIQETGLTIIYLATF
                     SYWLLDNSTEHLATRLFLENKLNMAKNLSNFWGFTYYAPSKIS"
BASE COUNT          523 a          355 c          344 g          636 t
ORIGIN      
        1 cattaaaaac attttttgcc tcgactttga ggtttttgta agtcctgttt ttgtaatcat
       61 tcacctctct tttacttcat ttctgcctca ataactaaag tacttattaa gtcacttgcc
      121 gacatagatc ggcataaatg agcagattgt ccagcccata acgacataaa atcaatatta
      181 tcgttttctc tggctttttg tctcattacc tgggttaatg cattttgaat ggggtagtca
      241 aggatattta ttttccgatt atccatgcaa gtaatgaatt tatttcgaat tccacgagca
      301 agctttccag aaaaagcacg tgttagcaca gtattatctt gttgttgtga taatagggtt
      361 tgtttgtatt tataagagat accagcttca aagcaactta aaaaagcggt tcccatttgt
      421 acaccagagg cgcctgaatt tatggcagag ataatgcctt taccattcat gattcccccg
      481 gcggctatca ctggcactct tattgtctca actagttgag gtatcagttc agatagttga
      541 atcaaagctt cctcagcatc gccgataaaa gtgcctcgat gccctcctgc ttcacttcct
      601 tgtgaaacaa tggcatcaat cccacttgct tcaagtatta ttgcttcgtg tatagtggtt
      661 gccgttccta tcagaaaggt tccatttttc ttcaattgtt tgatccacat tggttctaat
      721 gtaccgaatg catagctaaa gacagggatt ttttcctcta tcaggatttg catttggtca
      781 acaaaaggca gggagtaggg tttactcacg ggactgattt caatatttag ttcagtgcag
      841 cacaaattaa tatcatcaca agcgctttga atttgttctg gtgttgcatg atgtgcttcg
      901 ggtataaaaa gattcacggc aaaaggtttg cttgttaatt gacgaatttt tataatggct
      961 tgtctgatct catcgggtct catataccct gctcccagag agcccaaacc accactgtta
     1021 gaaactgctg caaccaactc aggagtagta gctcctccgg ccataggtgc ttgaatgata
     1081 gaaaattgaa taccaagtct ctcagctaat tttagatggt gcatagtaag taaaacccca
     1141 aaaattggat aaattttttg ccatatttaa tttgttttcc aaaaaaagac gagttgcaag
     1201 atgctctgtg gaattatcta atagccaata actaaaggtg gccagataaa tgatggttaa
     1261 tccggtttcc tggatggcac gttgaataaa ggtttctcga agaccagctg cttcacgcca
     1321 ccattgaaca gtttggctga tgcgtttgat tgcaggcaat tgaagatgaa gatgaccggg
     1381 ctctaattta tgaaatatca tttgccggat tactttttgc tggccttcaa aagtacttaa
     1441 ccaagtcatt aataaatcat gcagtttttc tttggcagtg agtaaatcaa attttggtct
     1501 ggagcacgca tttagcatgg cttcatcagc acgatcaaaa cagccatcaa tgatttgctc
     1561 tttttccgca aaaaaatatc ggatcgtatt taaatcgata tttaatgctt tagcgatatc
     1621 aatcagcctc actttttccc aagaggtatc cttggcaatg tctattgctg tattgatgat
     1681 ctcatttttt agtgttggtg ctattttttt catgaaacac ctccctgtct cttattagtt
     1741 tattactaat tttatgatca ggattaattt ttgatggttt tggtagttaa attaactata
     1801 agttaaatca aaaaatgcta ttggttattt caattagtct atgccacact tattttta
//
LOCUS       DATNSN010000284         1830 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.286, whole genome shotgun sequence.
ACCESSION   DATNSN010000284 DATNSN010000000
VERSION     DATNSN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.286"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            88..732
                     /locus_tag="VAH76_003101"
     CDS             88..732
                     /locus_tag="VAH76_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEO1387675.1"
                     /translation="MERFQDRYQAGQLLGEKLKPYANRNDVIVLALPRGGVPVAAMIS
                     EAINAPLDLMIVRKLGVPGHEELAFGALAINKIKVYNEELLQYLQLSQKDINQVISQE
                     ENELARRNRAYRGDKPFPDLKNKIVILVDDGIATGASMRAAVLALRKESPKKIIVAVP
                     VAPVDMNDIMKSVADEYICLISPISFNAVGLWYEDFSQTEDEEVLYLLNKSYNR"
     gene            complement(818..976)
                     /locus_tag="VAH76_003102"
     CDS             complement(818..976)
                     /locus_tag="VAH76_003102"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387676.1"
                     /translation="MDFEGWNSIVMDNAAFYKSEDAKRVLSSANCNLLFLPPHSPALS
                     PIGLSKQT"
     gene            complement(954..>1830)
                     /locus_tag="VAH76_003103"
     CDS             complement(954..>1830)
                     /locus_tag="VAH76_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387677.1"
                     /translation="ESKPVYFYLGCGKKEEIISELRKYLPENSLTEQQVNLEFDRLDK
                     NPGRTFNQECAQIQAKTLLMGGTYAFGCVITSSSRLGKNIFMKNKELACFPDKVYPNY
                     RRIKHYKPYLHENVVILPEITEGFNINSEIEDVRGMPYFRKYPSFAEVPSVFCEYFLE
                     LELCKSQALSKRPLEEQIQFINEGKARTYLPNPFLDYSDIYGRIATFSSNTKARLKKR
                     SYQALAWQEKTGTILSDLHKSSRTHRSMVASFFNAPEKKQESRVNEEPPTKKLKLDSP
                     QALTQQVNNGFRGME"
BASE COUNT          558 a          317 c          344 g          611 t
ORIGIN      
        1 taatcattat aaactcttgc tgacagcagg ataatttcca gatctgaact acaataaaag
       61 tagcaggaca gataattgag taggaaaatg gagcgctttc aagatcgtta tcaagctgga
      121 caattattag gagagaagct taagccttat gcgaaccgaa atgatgtcat agttcttgca
      181 ctgcctcgtg gaggcgtgcc tgtagctgca atgatttctg aggctataaa tgctccatta
      241 gatttaatga ttgttcgcaa actgggtgtt cccgggcatg aagaattagc tttcggtgct
      301 ttggccatta ataaaataaa agtttataat gaagagctgt tgcaatactt gcaactttct
      361 caaaaagaca ttaatcaggt tatttctcaa gaagaaaacg aattagcaag gcggaataga
      421 gcttatcgtg gtgataaacc ttttcccgat ttaaaaaata aaatcgtaat tctggttgat
      481 gacggtattg ccacaggcgc cagtatgcgt gcagctgttc ttgctttacg taaggagtca
      541 cctaaaaaaa ttatagtagc ggttcctgta gcacctgtgg atatgaatga tataatgaaa
      601 agtgttgcgg atgaatatat ttgtcttata tcccccatat cttttaacgc tgtgggtctt
      661 tggtatgaag atttttccca gactgaggat gaagaagtat tatatttgct taacaagtca
      721 tacaacagat aagtgtataa atgacaagtt ttgctgttta taaaatacaa atcggatcca
      781 attcatattt taacctcaga taggcataag ccagtcttta agtctgtttg cttaacccaa
      841 taggacttag agcaggtgag tgtggcggca aaaaaagcag attacaattt gcactactta
      901 gtactctctt tgcatcttct gatttataaa aagccgcatt atccataacg atgctattcc
      961 atccctcgaa atccattatt tacctgctgt gttaaggctt gtgggctatc taatttcagt
     1021 tttttagtag gaggttcttc atttacgcgt gattcttgtt ttttctcagg tgcattaaaa
     1081 aatgaagcta ccatgcttct gtgtgttctg cttgatttgt gtaagtcaga aagaatagta
     1141 cctgtttttt cttgccaagc taatgcttga taagagcgct tttttagtct tgctttcgta
     1201 tttgaagaaa atgtcgcaat cctaccatat atatcggagt aatccaaaaa tggattgggt
     1261 agataagttc tagcctttcc ttcattaatg aattgtattt gctcttctaa aggtcgttta
     1321 gataaggctt gagatttgca aagttctaat tctaaaaaat attcgcaaaa aacgctagga
     1381 acttcagcaa aacttggata tttacgaaaa taaggcattc ccctaacatc ttctatttcc
     1441 gaattaatat taaatccctc ggtaatttct ggaaggatta ccacattttc atggagataa
     1501 ggtttgtaat gcttaattcg gcgataatta ggatacactt tgtcaggaaa acatgccaac
     1561 tctttgtttt tcataaaaat attttttcct aaccggctgg agctagtaat aacacagcca
     1621 aaagcataag ttccacccat taataatgtt ttagcctgaa tttgtgcaca ttcttggtta
     1681 aacgttctgc caggattttt atcaagtcga tcgaattcta agtttacttg ttgttccgtt
     1741 aaactatttt caggtaggta ttttctaagt tccgaaataa tctcttcctt tttaccacac
     1801 cctaaataaa aatagactgg cttagattct
//
LOCUS       DATNSN010000285         1824 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.287, whole genome shotgun sequence.
ACCESSION   DATNSN010000285 DATNSN010000000
VERSION     DATNSN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.287"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            192..857
                     /locus_tag="VAH76_003104"
     CDS             192..857
                     /locus_tag="VAH76_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387678.1"
                     /translation="MTLILKMKKFKLMAKEQCFAFIKNAVNVGMDEVIYSNGTNKTFI
                     TDGLATCAAVAIYGSDSGCSAAFTHMSSEASDKDDKRKETILNEMMEYVLKSNSLSDV
                     KLIISPSRIQEKHLISFILEWVKQKKIACSMLETGGDSAVFNIDKDSRVLMLSTSLQL
                     KQDVSDKTWHGHGIIISQSEGIPVIDKQKKHFVFFAEDISTQITKFSDPPKTVGLAAA
                     VSA"
     gene            991..1416
                     /locus_tag="VAH76_003105"
     CDS             991..1416
                     /locus_tag="VAH76_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387679.1"
                     /translation="MRRHFIIIVLGIFQFGCIPLAEHHNKISPATMHEEYHDINFSNL
                     YEKNRSFPALETGIYAILDGILISSSQDTHLGKKQSKLLEEYVRQNRLFCQKGFDQEW
                     MNQLKQNLDRLEKTENNRIASAVNKKMQALFAERQCNTW"
BASE COUNT          603 a          274 c          359 g          588 t
ORIGIN      
        1 ggtcatttag tttatctaaa ctccctcatt aaaaacattt tttgccttga tctcggagtt
       61 ttttgtaagt cctgttgggt ttatgtaacg ggataacatc gataaatttt tcataaaaat
      121 tatacgattt tcttttcata agactcattt ttcatttaaa atggaaaaaa taatgacaat
      181 ttggttgata aatgactctg atattaaaaa tgaaaaaatt taaattgatg gcgaaagagc
      241 aatgttttgc ctttataaaa aacgctgtaa atgttggtat ggatgaggtt atctatagta
      301 acggtacgaa taagactttt attaccgatg gattggctac ctgcgctgct gttgctatct
      361 acggcagtga tagcggttgt tctgctgcat ttactcatat gtccagcgaa gcttcggaca
      421 aggatgataa acgaaaagaa acgatattaa atgagatgat ggaatatgtt ttaaaaagca
      481 attcattatc cgatgttaaa ttaattattt cgccttccag gattcaagaa aaacatttga
      541 tttcctttat tctggaatgg gtaaagcaaa aaaaaattgc ttgctccatg ctagaaacag
      601 ggggggattc agcagtattc aatatagata aggacagtcg tgtattaatg ctttcaactt
      661 cactgcaatt aaagcaggat gtatccgata aaacatggca tggtcatggt attatcatta
      721 gccagagcga gggtattcca gtgattgata agcagaaaaa acactttgta ttctttgcag
      781 aggatatttc cactcaaatt accaaatttt cagaccctcc taaaacagtt ggcttagcag
      841 cggcagtaag cgcttagaaa caagcataag ctgggtaaaa tgaaatgaat ttcgtattat
      901 agtcaaaatt gaaatatttc ctcaatgctg ggtagatatg ttaatttcat aatggattgt
      961 tttttatgct caatttgtag gtggtgtatg atgagaagac attttattat tatcgtgctt
     1021 ggtatatttc agttcggctg tatccctttg gcagaacatc ataataagat ttcacctgct
     1081 acgatgcatg aagagtacca tgatattaat ttttccaatt tatatgaaaa aaatcgctct
     1141 tttccagcat tagaaaccgg tatctatgct attttggatg gaatcttaat ttcctcaagc
     1201 caggatacgc atcttggtaa aaaacaatcg aaacttctgg aagaatatgt gcgtcaaaat
     1261 agattattct gtcaaaaagg ttttgatcag gagtggatga atcaattgaa gcagaatttg
     1321 gacaggttag aaaaaaccga aaacaatcgg attgcatctg ctgtaaataa aaaaatgcaa
     1381 gcgctgtttg ctgaacgaca gtgcaacaca tggtaaccag ggctcgccgt tttatcaggc
     1441 agcctggtcc aaatcacatg tgaactcaat ctttcgaacc aatgatcaca gtattttttg
     1501 tgcaggatat catgtcatga gaagggtggt tttggttcaa ttttcaactc cataccatgt
     1561 agctagggat aaaaacggga aagacaagca attaatactg tatttttatc aatcatattg
     1621 attgttattt aattatttag tataataata aacttgatta ggttatggct ctattgtctt
     1681 ttagtgagat gtgctaatgg aattcgaaga ttggtttctg gaacaaataa gcaatggtaa
     1741 aaaatgcatc ttgatagggg aaaaccatta ttcctatgca agcgaggact gcctcgattg
     1801 tattttaaaa ttgcaaaggc aagg
//
LOCUS       DATNSN010000286         1823 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.288, whole genome shotgun sequence.
ACCESSION   DATNSN010000286 DATNSN010000000
VERSION     DATNSN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.288"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            205..1221
                     /locus_tag="VAH76_003106"
     CDS             205..1221
                     /locus_tag="VAH76_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO1387680.1"
                     /translation="MDIQNNVTQNHQPKRAISDVADFLGISMQAVHKQLKNKNINCEK
                     IGNKSFLTFESARELFNFKFKRKKIAVQIVKGGTGKTTTINNIASCANTYGARVLLID
                     ADPQGNLTDANGIDAEEYPVLIDLLKDGASIQESIVTVSQGLDLIPSRIENVILDNEI
                     VNGRYPLEKLYNNLLSKVENYYDFILIDCPPTMGQAVTAASLYADIILAPLNPDKFSA
                     KGLKILKQEVDTLNKHFHKKIEYKVFLNKFSSKTILSDKAIVSLISDPELEGKVLSTT
                     VQYAQEIPNITDDNKSSFSNLKKSLVRDDFDRLTRELLEINAETFAKDERALSKNLKE
                     ESLA"
     gene            1235..>1823
                     /locus_tag="VAH76_003107"
     CDS             1235..>1823
                     /locus_tag="VAH76_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387681.1"
                     /translation="MPDLEELLQSRSKKKFMKKSYRPWDLSGGPTSLTEKNEFGTTTL
                     ENLVHQKDSVVKNNLSVVAQCISQENTHSTLDNDKDIGKITNKYQSGTDSENHGISNE
                     YQMDNSTDTIKETTRNHLDNNLDNKETSNNQSILEKFIKETSEITNNFNENLEYDHNE
                     IDLISPSKIEQEIIRLSGKQKLIFDMVIEICSARKC"
BASE COUNT          747 a          281 c          287 g          508 t
ORIGIN      
        1 atatcattca tatgtgatta tgatttacaa aaaaaatcca tcttgatcgg aaggtaaaaa
       61 cattataaac atttgtaaaa cgtcaacatt aaataatatt gaaatcttcg taaaattgca
      121 tttttatcat tactttatag tttacatggt ttacgtttta tgattaaaat catacaaaac
      181 atcaaccaca taatgagatt caatatggat atacaaaaca atgtcactca aaatcaccaa
      241 cccaaaagag caatatctga tgttgcagat tttctcggca tctcaatgca agccgtccat
      301 aaacaattaa aaaataaaaa tataaattgc gaaaaaatag gtaacaaatc ttttttaaca
      361 tttgaatcag ctagagagtt atttaatttt aagttcaagc gaaaaaaaat cgccgttcaa
      421 attgttaaag gtggaactgg aaaaactaca acaatcaata atatagctag ttgtgccaat
      481 acttatggag caagagtgct attgattgat gcagatccac agggtaattt aacagacgca
      541 aatgggattg acgcggaaga atatcctgta ttaatagatc tattgaaaga tggagcatca
      601 attcaagaaa gcatagtaac agtttcacaa ggattagatc taataccaag ccgcattgag
      661 aatgtaattc tcgataatga aattgtcaac ggaaggtatc cattagagaa gctttataat
      721 aatttattaa gtaaagtcga gaactattat gactttatat tgatagattg cccgccaact
      781 atgggacaag ccgtaactgc cgctagtctg tacgctgata ttattctagc ccctttaaat
      841 cctgataaat ttagcgctaa agggttaaaa atattaaagc aagaagttga tacattaaat
      901 aaacatttcc ataaaaaaat tgaatataag gtatttttga ataaattcag ttcaaaaaca
      961 attttatctg ataaagcaat cgtatcactt atttctgacc cagaattaga agggaaggta
     1021 ttatctacta cagtacagta tgcgcaagag atacctaata ttacagatga taacaaaagc
     1081 tcatttagta atttgaaaaa atccttggta agagatgatt ttgatagatt gacacgagag
     1141 ttacttgaaa taaacgcaga aacttttgct aaagacgaac gagcattatc caaaaattta
     1201 aaagaagaat ctttagcata gggagagcta taatatgcct gatttagagg aacttctaca
     1261 gtcgcgtagt aaaaagaaat tcatgaagaa atcctacaga ccatgggact tatcaggtgg
     1321 acccacatcc ttgaccgaga aaaatgaatt cggaacaaca actttagaaa atctagttca
     1381 tcaaaaggat tctgttgtta aaaacaatct atcggtagtt gcccaatgca taagtcaaga
     1441 aaatacacat tccacattag ataacgataa agatatcgga aagataacaa acaaatatca
     1501 atcaggtacc gattcggaaa accatgggat atctaatgaa taccagatgg ataactcaac
     1561 tgataccatt aaggaaacta caaggaacca tttagataac aacttagata acaaagagac
     1621 atcaaacaat caatcaattt tagaaaaatt tataaaagaa acatccgaga ttacaaacaa
     1681 tttcaatgaa aatcttgaat atgatcataa tgaaatagac ttaatttcac cttcaaaaat
     1741 agaacaagaa attatacgcc tttcgggaaa gcaaaaactt atatttgata tggtcataga
     1801 gatatgctca gcaagaaaat gct
//
LOCUS       DATNSN010000287         1799 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.289, whole genome shotgun sequence.
ACCESSION   DATNSN010000287 DATNSN010000000
VERSION     DATNSN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.289"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            46..954
                     /locus_tag="VAH76_003108"
     CDS             46..954
                     /locus_tag="VAH76_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387682.1"
                     /translation="MNIADLQSFLAVVELHSISLAAKKLHITQPALSKRIRKLESEWN
                     TQLFISSGLRTELTDKGKQLLPYVRQMVYLSHELISSTGTDKKKPQLLLNIGTTVYIA
                     QTIIPPLIIHMNALDLNYFINTKLIADKDVAHGLSEGAYDLIISPFMSNSAEIKSVPL
                     WRERLIPIVGLSHPLAKIKEKLSFAELAEFDAVLMEKNFIIRRIIDKEAEKQKLTLKI
                     RAEANIIYNNIALVEQGMAWSMINERLLNPNLKPLELSDYALSIDFYCHFLQKRTDER
                     LIRIFIDNLVNWVNTSSELSSFSLVN"
     gene            complement(964..1185)
                     /locus_tag="VAH76_003109"
     CDS             complement(964..1185)
                     /locus_tag="VAH76_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387683.1"
                     /translation="MDNGGTLEGVSRLFSVGTTTIKNWKRLRAQTGKLEGSGPPCNPY
                     KIDADALKLYLKERPDAFLNGNPPIFNWQ"
     gene            1286..1447
                     /locus_tag="VAH76_003110"
                     /pseudo
     CDS             1286..1447
                     /locus_tag="VAH76_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          581 a          322 c          307 g          589 t
ORIGIN      
        1 tatcctagta ttaatttaag taatattatg attggtttta aaataatgaa tattgccgat
       61 ttgcaatctt ttcttgcagt tgtagaactc cactctatct ctttagctgc caaaaaattg
      121 catattactc agcctgcttt aagtaaaaga attagaaaac ttgagtcgga gtggaatacc
      181 caacttttta tttcttcagg attaaggact gagctaactg ataaaggaaa acaattattg
      241 ccttatgttc ggcagatggt ttatctgagc catgagttaa ttagcagtac tggtacagat
      301 aaaaaaaaac ctcagttact gctaaatatt ggtaccacag tatatattgc tcaaactatt
      361 atcccccccc ttataatcca tatgaacgca ctggacttga actacttcat taatacgaaa
      421 ctgattgctg acaaagatgt agctcacggt ttaagcgaag gtgcttatga tctgattatc
      481 tctccgttca tgagtaattc tgctgaaatt aagtctgtac ctttgtggcg agaaaggctc
      541 atcccaattg tcgggttatc acatcctcta gcaaaaatta aagaaaaact atcctttgct
      601 gaattagcag aattcgatgc ggtgcttatg gaaaaaaact ttataatacg ccgaattatt
      661 gataaagaag ctgaaaaaca aaagttgact ctcaaaataa gagctgaagc caatattatt
      721 tataacaaca tagctcttgt cgaacaaggt atggcttgga gtatgattaa tgaacgctta
      781 ttgaatccta atttaaaacc tctcgaacta tctgattatg ccctcagcat tgatttttat
      841 tgtcattttc ttcagaaacg tacagatgaa cgattgattc gtatttttat tgataatctg
      901 gtcaattggg ttaacacgtc aagtgaatta agctcttttt cccttgttaa ttagataaac
      961 cctttattgc cagttaaaaa tggggggatt accgtttaga aaagcatcag gacgctcttt
     1021 taggtataat tttaatgcat cagcatctat tttgtaggga ttgcaagggg gacctgaacc
     1081 ctctagcttc cctgtttgtg ctctcagacg cttccagttt tttattgtag tggtccctac
     1141 tgaaaacaat ctacttactc cttctaacgt gcctccgtta tctatatgcc gtaacacgtt
     1201 ttctcaaagt ctaatgaata ggtcataaat taaaaagcgt cataataaca aattacttgg
     1261 gcatttcaaa ttttaaatca gtatagttca ttggcgtaat tatcaaacca gacgggaggc
     1321 aaaacaagat attctggatt acattactat gttttataac aatcaaagat tgcattcgtc
     1381 tttggattat ctaagtccga atcaatttga aaatcggtac tgggggttaa tgaaaaaagg
     1441 ggcttaactg ggatgtgcaa attggattga ccacttcatg atttagtcga ttaggagaat
     1501 gctgcctgat ctatttccat catacataac ttttaactgt agctcatcga ccattcatat
     1561 tcccaataca tcctttcagt cacgatctaa agaataaaat tttgttccat actttaggag
     1621 gatacaacag taatagccta catcgatagc aaaaaccagc agcaactctc ttttagtcat
     1681 ttacgtttgc cgaaaacgtc acagtgcatt cagaatattt gattatggaa attaaatgct
     1741 tactcggtca gatactgatt ttagagaatc aattaaattt tagtcaatta cagaattaa
//
LOCUS       DATNSN010000288         1788 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.290, whole genome shotgun sequence.
ACCESSION   DATNSN010000288 DATNSN010000000
VERSION     DATNSN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.290"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            192..1385
                     /gene="dupB"
                     /locus_tag="VAH76_003111"
                     /gene_synonym="laiF"
     CDS             192..1385
                     /gene="dupB"
                     /locus_tag="VAH76_003111"
                     /gene_synonym="laiF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupB/LaiF"
                     /protein_id="HEO1387684.1"
                     /translation="MLYNKSSFCLWKETIVPIILDSDVLEVAEYVYKTRLSQPYTEVG
                     SEWEYNYKKPTATYAKGDGHNLQRYITIDGKQLHRPIHGLAHTMRTLMYSQLMHCSSK
                     KQLFPHVCQDGRTIADLSELDLKKINIAQLFFVAGRESEASYGDAYHRYHLYGAKQFE
                     EYARKHLTHLFSEEEIQLYSRCIEDRVGDSFDGTPEGYIIHLSHMIDLMRCKSPVEVF
                     LGHSKGVSGIVPTLIHLFGKQDGLDIMHYARGLFAATGEAVPYIDSSEWPHLGVDLSR
                     VQRALSIVGDINVPGQEADPKKTAQAGFSVDGCYSALTSVPTPSWYEKELKEVDDEKI
                     DVKEVDDREIEKEHEIVVPSQATTPLSTEKPGSFVEKFLKPFMIWKKPELQTTQPTTE
                     KTNRP"
     gene            1457..>1788
                     /locus_tag="VAH76_003112"
     CDS             1457..>1788
                     /locus_tag="VAH76_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387685.1"
                     /translation="MFSYLDKLLDGIFGNQTPDTSQLSTSKSPALTTVPLKQEYFVKI
                     GESDTQDLGLLPVVSKRHNQSVPLEEIPFEETRLELIGLYITLLKQCVLDEKLKSIPA
                     QYLISHYL"
BASE COUNT          552 a          326 c          375 g          535 t
ORIGIN      
        1 taagtcggat tgttaaaaat ctgataagat ttatgttttt tcttcccgca taaaaaacaa
       61 tgattgttat tctataaaat aatcaagttc acaacttgag ttatccaaaa cacttgaata
      121 taagattttc gattaaatca ttgcggtttt ttgctttata cctattgcaa tgaattgata
      181 aataattaaa cttgttatat aataaatcct cattttgttt atggaaggag accattgtgc
      241 ctattattct agattctgat gtgttggaag tggcggaata tgtgtataaa acacgcctat
      301 ctcagcctta cactgaagtc ggttctgaat gggagtataa ttacaaaaag ccaacagcta
      361 cctatgcaaa aggcgatggt cataatttgc aaaggtacat tacgattgat ggcaagcagc
      421 ttcatcgccc tatccatggc ttagcccata cgatgcgcac attgatgtat agccaattga
      481 tgcattgctc ctcaaaaaaa caacttttcc cacacgtatg ccaggacgga cgaacaatag
      541 ccgatctcag tgaacttgat cttaaaaaga taaacatcgc gcagttattt tttgtagccg
      601 ggcgcgaaag cgaggcttct tatggtgatg cttatcatcg ttatcatctg tatggggcta
      661 aacagttcga agaatatgct cgcaagcatt tgactcatct tttctcagaa gaagaaatcc
      721 aattgtactc acgatgtata gaagacaggg taggtgacag ttttgatggt acccctgaag
      781 gttatataat tcacttgtca cacatgatcg atttgatgcg ctgtaaaagc ccggttgaag
      841 tttttttggg gcactcaaaa ggggtttctg gtattgtacc tacattgatt cacttgtttg
      901 gcaaacaaga tggcttggat attatgcatt acgcgcgagg cttgtttgcg gctacaggtg
      961 aagcagtacc ctatattgat tcatctgaat ggcctcattt gggagttgat ttgagtcggg
     1021 tacaacgtgc tctctctatc gttggtgata tcaatgtccc tgggcaagag gcggatccta
     1081 aaaaaacagc gcaagccggt ttttcagtgg atggttgtta ttccgcgtta accagtgtgc
     1141 ctacaccttc ctggtatgag aaagagttaa aagaagtcga tgatgaaaaa atcgatgtta
     1201 aggaagttga tgatagggag atagaaaaag aacatgagat agttgttcct tcgcaggcaa
     1261 caacaccttt aagtacagaa aaacccggct cttttgtcga aaaatttctg aagccattta
     1321 tgatttggaa aaagccagag ttacaaacga ctcagccaac aacggagaag actaacagac
     1381 cttaacctgt tttacttaaa gtagttagag aaacgaattc tcctagggaa ttaaaacgca
     1441 ctgaggtgtt gtaactatgt ttagttatct ggataaatta ttagatggaa tatttggtaa
     1501 ccaaacgcct gataccagtc agttaagcac ctcaaagtca cctgctttaa ctactgttcc
     1561 cttgaaacaa gaatattttg taaaaatcgg ggaaagtgac actcaggatc ttggcttact
     1621 gcccgtggtt agcaagcgac acaatcaatc agtgcctctg gaggaaatcc ctttcgagga
     1681 aaccaggctt gagttgattg gactatatat tacattgtta aaacaatgtg ttttggatga
     1741 aaaactgaag agcatccctg cgcagtatct tatatctcat tatctttt
//
LOCUS       DATNSN010000289         1763 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.291, whole genome shotgun sequence.
ACCESSION   DATNSN010000289 DATNSN010000000
VERSION     DATNSN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.291"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(2..751)
                     /locus_tag="VAH76_003113"
     CDS             complement(2..751)
                     /locus_tag="VAH76_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEO1387686.1"
                     /translation="MSNNIPERVWDTIYAFLQETKGLHIKNKKKTRRFLEGVWYVMRT
                     GCQWRLIPNYYGHWRQIHKRYKLWADRGIWCNLMSFVSDIDDQAVMLDATIVRAHACA
                     SGYIKDGNDAHALGRSKGGFTTKIHALVDGLGNPIKFILTGGHRNDITQAAELLQEQH
                     DTVVLADKGYDSQALIDTLEEQGCSAVIPSRKSAKKPRKIDTEQYKERFLIEAFFSKI
                     KHFRRIFSRFDKTTSAFAGFLHFAGALIWMR"
     gene            848..1318
                     /gene="istB"
                     /locus_tag="VAH76_003114"
                     /pseudo
     CDS             848..1318
                     /gene="istB"
                     /locus_tag="VAH76_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family helper ATPase
                     IstB"
BASE COUNT          559 a          356 c          298 g          550 t
ORIGIN      
        1 tctaacgcat ccatatgagc gcaccggcaa aatgcaagaa gcctgcgaaa gcggaagtag
       61 ttttatcaaa acgagaaaaa attcttcgga agtgctttat tttagagaaa aaagcctcaa
      121 ttagaaatcg ttctttatac tgctctgtat cgatctttct tggttttttt gcactctttc
      181 ttgacggaat gacagcagaa caaccttgct cttccaatgt gtcaataagg gcttgtgaat
      241 catatccttt atcagcaagg actactgtat cgtgctgctc ttgtagcagt tcagccgctt
      301 gagtaatatc atttcgatga cctccagtca gaataaattt aattggatta cccagcccgt
      361 ctaccagcgc gtgaatttta gtggtaaagc ctcctttgct gcgtcccaat gcatgagcat
      421 cgttcccatc cttgatatag ccagaggcac acgcatgggc acgcactata gtggcatcga
      481 gcataactgc ctggtcatca atgtcactaa caaaagacat taaattgcac caaataccac
      541 gatctgccca gagcttatat cgcttgtgga tttgacgcca atgcccataa tagtttggga
      601 tcaaacgcca ttgacagccc gtacgcatta cgtaccagac tccttctaaa aaacgccttg
      661 tcttcttttt attcttaatg tgcaaccctt ttgtttcttg aaggaatgca taaattgtat
      721 cccatactcg ttctggtata ttattgctca tgtaggcagt tccattagtg ctttttacga
      781 tcgtcaatcg aatttcacta taaaaactgc ctatctttca actaattaat taaatttgtt
      841 cacagaacct agtggattag gcaagactca tttagcgatt gctctgggtt atttagcagt
      901 tcaagcaaac attaaaacaa aatttattag cgcagcagat ttgatgttat tgcttgaaac
      961 agctcatcgc caagggcgat ataaagatat tatgcgacgg gttattaata atgctcgact
     1021 gctcattatt gatgaaattg gatatttacc aatgaaccga gatcaggcaa atcatttctt
     1081 ccaagttatt gcaaatcgct atgaaaaagg ttctattatt gccacctcca atctcgcttt
     1141 tggtcaatgg gacaatactt tcgccggcga taaagtatta acagcagcta tgctcgacag
     1201 gttgctccat catgctcatg tcattcaatg cagaggcgac agttatagat taaaagacaa
     1261 atttaaggcc ggtttaattc aaaatcagaa ggaggagcaa aataatataa ctgattaatt
     1321 ttttaccagg gggatcaatt ttcatctggc aaaaggatca attttcaatc ggcattgaca
     1381 gttaggttta cttcataaat caccataatt tatcttccca aattcccatg atttgaattc
     1441 accaattgca cacaacaact gaatttattt ttagaataat gagtcatgcc atgattggat
     1501 acgatctacc atttggctat aaacaaataa taaattgtat tttaattcac actttagtta
     1561 cttgaatttt aaatgaccga ttatcggttt gccgctgatt tacaatagta cccataaagc
     1621 atactcagca taccttgaac aagtcaaaga atgtagacta tttgtcaaaa catttaaaaa
     1681 tcaaataaca atatgaataa cgcaatattc ccaccaaatc tttattaaaa aaaagttcca
     1741 tattatttta ttttaaatga taa
//
LOCUS       DATNSN010000290         1727 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.292, whole genome shotgun sequence.
ACCESSION   DATNSN010000290 DATNSN010000000
VERSION     DATNSN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.292"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            428..1270
                     /locus_tag="VAH76_003115"
     CDS             428..1270
                     /locus_tag="VAH76_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387687.1"
                     /translation="MKIKLSILLFLLMVLKTYASTAFFPKTINVWNSKEQAKIIGNCE
                     NQDKGSSDNSLPKRIRCQFTQISIKHEPPIKSKMNAKETLEFYLNSRKVKSIEELKRI
                     LCAEFNKSPELEDLSDPFGKKRYQSFVHLCQKDGNISEFIKSEVDNWSENIVNIKECS
                     LSTQIYSLDFKHSSTNNDWISTTEPQRNFCGMIVVTRLHLDPESPFWHYSDTHIVTNP
                     EDSFGTLPSADNSCSKLPKSTTTTFYGVQKFIKEKGFNANDISSSRNTIFVKCDRLNL
                     LNSI"
BASE COUNT          594 a          279 c          275 g          579 t
ORIGIN      
        1 aacaaaataa caagtgaaaa catattagtc aataatttta aatattacat taagaagtat
       61 gaccaccgct ttttatttat tggcattagt ggagtttaaa aaatatacca agaaaaattg
      121 cctctcgaat tacaaaaact tgaatcttag tttgcaagat gaatagttaa agtcgctcaa
      181 tatatctcta ttaaattacc tgaggcatgt gggtgtaatt taaatgtgac tgatgcagct
      241 cctcggtttg ctgaatttta aagtttgatg atttagcatt atttgattta aataaatcgc
      301 aattgatcgg aatataatac agatcattat aatgcttctt tattacttta tctgagcact
      361 agagtttgac ttgtatccaa tacgtggttg atttattatc gtcagcctgg acaattttgg
      421 atcatccatg aaaatcaaat tatcaatcct tttattcttg ttaatggttt taaaaactta
      481 tgcatctacc gctttttttc caaaaacgat aaatgtatgg aactcaaaag aacaggcaaa
      541 aataataggt aattgtgaaa atcaagataa aggatcttcg gataattctc ttcctaagag
      601 aatccgttgt cagttcacac aaattagtat taaacatgaa cctccaatta aatcaaaaat
      661 gaatgccaag gaaacactag agttctatct gaattctcgc aaagtgaagt ctattgagga
      721 attaaaaaga atattatgcg cggagttcaa taagagccca gaactagaag atttgagcga
      781 tccatttggt aaaaaaagat atcaatcttt tgttcattta tgccaaaagg atgggaacat
      841 aagtgagttt attaaatcag aagtggataa ttggtctgaa aacatagtta acattaagga
      901 atgctcacta tcaacgcaaa tttactcttt agattttaag cattcatcta caaataatga
      961 ctggatttct acaacagaac cacagcgcaa tttctgtgga atgattgtgg tcacacggtt
     1021 gcatctcgac ccagaaagtc ctttttggca ttactcagat acccacattg tcacaaatcc
     1081 agaagatagt tttgggacat tgccatcagc ggataattct tgtagtaagt tgccaaaatc
     1141 aaccacaacc actttttatg gagtacaaaa attcatcaaa gaaaaaggtt ttaatgccaa
     1201 tgatatatct tcctcgagaa atactatctt tgttaaatgc gatagattaa atctattaaa
     1261 ttcaatataa aattattgct cttgcagtat cgatttggtt cctaggactt aaggaaactg
     1321 gggaatggcg agcatggatt tcttttatct aatcccaagt taattaaatg agggtttatt
     1381 ttatgaatag atacatttac ctatgtaaaa gacttagaat aagtgcaatt atagatatca
     1441 acaccgaaat aaaagcatag atgttcgccc attttgcttt ctcttgaggt ttttccgcta
     1501 aatcatttga tttgattgca caggccagca ttttttcgtt atcaatttcc tcttgtcttt
     1561 ctataatatc tctctttttt actccgccaa tagttctttt tgatttaaaa gtcttcgtat
     1621 ttcctcagga ctcttggttt gtatttcagc ccggtatttt aatcgctgtg tattattagc
     1681 tctaaaaata ctctcgccaa caaaataaat agaaatgagc aaataaa
//
LOCUS       DATNSN010000291         1704 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.293, whole genome shotgun sequence.
ACCESSION   DATNSN010000291 DATNSN010000000
VERSION     DATNSN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.293"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            221..451
                     /locus_tag="VAH76_003116"
     CDS             221..451
                     /locus_tag="VAH76_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEO1387688.1"
                     /translation="MTKGKSHHVVPNGNKGWKVIKDAANRASGSYKTKKEAVDAGRKI
                     SQNQKTELVIHGKDGKIRNKDSHGNDPYPPKG"
     gene            455..730
                     /locus_tag="VAH76_003117"
     CDS             455..730
                     /locus_tag="VAH76_003117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387689.1"
                     /translation="MVTNKIMEEIYQSASQVKEKIYSAFRYTAISINLINRSTTKDYD
                     ISDITKISINLNADHDDVKSKYISWIIGNCLTDMFEIFGSTPIKGAL"
     gene            783..>1704
                     /locus_tag="VAH76_003118"
     CDS             783..>1704
                     /locus_tag="VAH76_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO1387690.1"
                     /translation="MPRKNLILNLPGFSIVKVSGYQPLLLDVRYNRLARCSHCQSKKV
                     RKKASYIRRVHHELIGHRRSILRFKAYKLYCYACGRYGNQQFPGINKHQRATWRVQAA
                     VFHEHSRGVSQKDLSERYKKGKATIERWYQRHYEEQHRELLNKPCPVVLGIDEHFFSK
                     KEGFATTLCDLRKQKVFDVVRGRSEGDLKDYLQQLPGKERVKVICMDLSSTYRSIVKK
                     YFPNAMIVADRFHVIRLIQHQCMMTYRELSSDIKSNRGILALLRTRPDNLTEEKKAKR
                     DAFLTQNPAIEAIYQFQQELHSLLMKRALTQ"
BASE COUNT          617 a          267 c          324 g          496 t
ORIGIN      
        1 atttttgatc tattttggtt cttaattcct taatggtttt gctagttaaa ccctcacaag
       61 agcgtttctc tttaagccct acatcatcag cctcaagttt atatgctaca aaattttcat
      121 ccatgactat ctccaaattt aatcgaacca ttcaagctta aattcatttg tttctataat
      181 aggctatatt tcaaatatta gtactacaag gaggaatata atgacaaaag gtaaatctca
      241 tcatgtagtt cctaacggaa acaaaggatg gaaagttata aaagatgctg ctaatagagc
      301 aagtggttca tataagacta aaaaagaagc tgtagatgct gggaggaaaa taagtcaaaa
      361 ccaaaaaaca gagttagtta ttcatggaaa agacggaaaa attcgaaata aagatagcca
      421 tggaaatgac ccatatcctc ctaaaggcta aaatatggta accaataaga taatggaaga
      481 aatttatcag tcggctagtc aagttaagga aaagatttat agtgcatttc ggtatacggc
      541 aatatctata aatttaatta atagatcaac gacaaaagat tatgacatat cagatataac
      601 taaaatatca attaatttaa atgcagatca tgatgatgtt aaaagtaaat atatctcttg
      661 gataattgga aattgtctta ctgatatgtt tgaaatattt ggctctaccc caattaaggg
      721 ggcactttaa tcaaattagt ctagactaaa tccttgattt tgcgatcaaa aaggagatta
      781 aagtgcctag gaagaatctt atcctaaatt tacctggatt ttccatagta aaagtgagcg
      841 gatatcaacc attattatta gatgtgagat acaaccgctt agcgcgttgt agtcactgtc
      901 aaagtaagaa agtacggaag aaagcatcgt atatacgaag agttcatcat gaactaatag
      961 gtcataggcg aagtatatta aggtttaaag cgtataagtt gtattgttat gcgtgtggtc
     1021 gttatggaaa tcaacaattc cccggtatta ataaacatca acgtgctact tggcgagttc
     1081 aggcggcggt gtttcatgag catagccgag gagtatctca aaaagactta tcagaacgtt
     1141 ataagaaagg caaagcgacc atagaacgtt ggtatcaacg tcactatgaa gaacaacata
     1201 gagaactact gaataagccc tgtcctgtag tgcttggtat tgatgaacat ttcttcagta
     1261 agaaagaggg ttttgctact acgttatgtg acctgagaaa gcaaaaggtc tttgatgtag
     1321 ttcgtggccg aagtgaaggg gatttaaaag actatctgca gcagctgcct ggaaaagaac
     1381 gagttaaagt gatctgtatg gacttaagca gtacctatcg gtctattgta aaaaaatact
     1441 ttcctaatgc aatgatagtt gccgatagat tccatgtaat ccgtttaatt caacatcaat
     1501 gtatgatgac ttatagagaa ctctccagcg acataaaaag taataggggc atcttagctt
     1561 tattaagaac tagaccagat aacttgactg aagaaaagaa ggctaaaaga gatgcatttt
     1621 taactcaaaa tcctgctatt gaagccatct atcaatttca gcaagagctt cattctctgt
     1681 taatgaaaag agctttaact caac
//
LOCUS       DATNSN010000292         1697 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.294, whole genome shotgun sequence.
ACCESSION   DATNSN010000292 DATNSN010000000
VERSION     DATNSN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.294"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1045
                     /locus_tag="VAH76_003119"
     CDS             <1..1045
                     /locus_tag="VAH76_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1387691.1"
                     /translation="PINFTIGFVFILILLLTSTFTYKFVELPFRNKLKLSFLNTMLLF
                     FILPLALAVIFYLFSQYKDNFGFNKISPEKLNQVNTYYGLLKQDYGCIDNKDGFSVLP
                     SEKLCTIGDYHSNKKTVLVVGDSHATAYVGMLHVLLSDQHLQAYVVNQSGTPFILGNI
                     SNWRENNPMSRNELLRKLIKNKKYDYVVMGGFWDYYPDLPSLDGKKHPPFEVFKKGLR
                     EAVKFIVDNKSIPVIMFDNPPLINLSKTCGLTRVSFLDCSNNLREVKKIQNATRNIIL
                     NLKNEFPQLILIDPTKIICDKSKCYTSINGTPLYFDEGANSHLNYDGSTLIGKLYLKK
                     FGNPFQTKNSIYS"
     gene            complement(1171..1365)
                     /gene="csrA"
                     /locus_tag="VAH76_003120"
     CDS             complement(1171..1365)
                     /gene="csrA"
                     /locus_tag="VAH76_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEO1387692.1"
                     /translation="MLILTRRIGETLIIGDDVNITVLGVKGNQVRLGINAPKDVSVHR
                     EEIYLRIQQEKESDDSEQAV"
BASE COUNT          588 a          288 c          253 g          568 t
ORIGIN      
        1 cccaatcaat ttcacaattg gatttgtttt tatattgatt ttgctattaa cttcaacctt
       61 tacttacaaa tttgttgagc ttccgtttag aaataaatta aagctttcct ttctcaacac
      121 aatgctactt ttttttattc ttcctcttgc tcttgcagtt attttttact tattttctca
      181 atacaaagat aattttggat ttaataaaat ttctccggaa aaacttaatc aggtaaatac
      241 ctattatggg ttgttaaaac aagattatgg ttgcattgat aataaagatg ggttttcagt
      301 tttaccatca gaaaaattat gtactatagg agattaccat tccaataaaa aaactgtttt
      361 ggtagttggt gacagtcacg caaccgcata tgttggtatg ttgcatgtgt tgctgtccga
      421 tcaacactta caagcttatg tggtcaatca atcaggaacc ccctttatat taggaaacat
      481 ctcgaattgg agagaaaaca atcctatgag ccggaatgag cttctcagaa agttaattaa
      541 aaataaaaaa tatgattatg tcgtaatggg cgggttttgg gattattacc ctgacttgcc
      601 atccttggat ggcaaaaaac atcctccttt tgaagtattc aagaaaggat tacgtgaagc
      661 agtaaaattt attgttgata acaaaagtat cccagtaatt atgtttgata atccaccctt
      721 gataaacctg agtaaaactt gcggattgac aagggtatcc tttctggact gctccaataa
      781 cttacgtgag gtaaaaaaaa ttcaaaatgc taccagaaat attattttga acttaaagaa
      841 tgagttccca cagcttatat taattgatcc gacaaaaata atatgcgaca aatcaaaatg
      901 ctatacctca attaacggga caccattata tttcgatgaa ggtgcaaatt ctcatttgaa
      961 ctatgatggt tcgacactta ttggaaaact atatctcaaa aaatttggta atccatttca
     1021 aacaaaaaac tcaatatata gttagtatta attttgattt gctgatgagt gtataagaag
     1081 ctttattaaa gaggaaatta catttagtta catagtgcgt cccgagtatt tttaagtact
     1141 cggaactgga tgagctgcgt ggaaatcgat ttatactgct tgttccgaat catcagattc
     1201 tttttcttgt tgtatgcgta aataaatttc ttcacgatga acagaaacat cttttggagc
     1261 attaattcct aagcgaactt gattgccttt tacacctaaa acagtaatat tgacatcatc
     1321 acctatgatt aatgtttcac ctatacgccg agtcaaaatc aacatgacta aaatctccct
     1381 aacatagcag ctacctactt ctccatagaa cgcttccatg gtgttcttgg caactacaat
     1441 atgaacaaaa tttaagcaaa atttattgct tggatcacat tgtacactct gattattaac
     1501 ggaatattaa gtcatagata aatttgcgtt taatttttgc agaaatgagt tgaaaaatta
     1561 ataaattcct tgtcataaat tcatcataaa actgttcaac attccctaat atcaaaaact
     1621 cccaattatt aataaactgg actcaaaagg aaaaacaagt ttttttcctg caataaactc
     1681 aaacaatata aaaatac
//
LOCUS       DATNSN010000293         1694 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.295, whole genome shotgun sequence.
ACCESSION   DATNSN010000293 DATNSN010000000
VERSION     DATNSN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.295"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..929
                     /locus_tag="VAH76_003121"
     CDS             <1..929
                     /locus_tag="VAH76_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO1387693.1"
                     /translation="GYVIKNRKKPLQIKQQIEADIIPLLGHLELEKIKPIDITNALDA
                     IVKRGAPIHANRVLSSLKQVFNYAISRGSLETNPAANIKARNIGGIEKPSERFLALDE
                     IKTLWQFLNSDQSKMSLQTKSAIKIILLTGVRTAEIRLAQWSHFDFANSLWTIPSELT
                     KGGITVKIHLSELTKSVIFKLNEGSDSPFVLSGLVPNKPLDENALPRAIRRIQKRVGI
                     PEWSAHDLRRTFATQLGETLHIDPVVIEKCLGHKMPRIMATYNKNEMLPQRKEALERW
                     SKYIQELVGHSEENSSSQSNLILQTSKNLCLI"
     gene            complement(1197..1415)
                     /locus_tag="VAH76_003122"
     CDS             complement(1197..1415)
                     /locus_tag="VAH76_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEO1387694.1"
                     /translation="MRPVNDLFTQQILFINDVEVIIGKDRLTLRRWWTDGKFPLPVKL
                     HGRTLVWHKEVIDEWISTNIRKGYNQAL"
BASE COUNT          578 a          314 c          276 g          526 t
ORIGIN      
        1 aaggttatgt aattaaaaat agaaaaaaac cacttcaaat taaacaacaa atcgaagcag
       61 atattattcc cttgctcggt cacctggaat tggaaaaaat aaaacctatt gatattacta
      121 acgcacttga tgctatagtt aaacgaggtg cgccaatcca tgcaaatcgg gttcttagtt
      181 ctttaaaaca agtctttaat tacgcaatta gccgcggcag ccttgaaact aaccctgctg
      241 ctaatattaa agcaagaaat attggcggaa ttgaaaaacc tagtgagcgt tttttagctc
      301 tcgatgaaat taaaactctt tggcaatttc ttaactccga ccaaagtaaa atgtccctgc
      361 aaactaaaag tgctatcaaa attattcttc tcacaggtgt tagaactgct gaaattcgac
      421 ttgctcaatg gtcacatttt gattttgcta attccctatg gacaattccc tcagaactaa
      481 ctaagggcgg aatcactgtc aaaattcatt tatctgagtt aacaaaatca gtaattttca
      541 agttaaatga aggaagtgac tctccttttg ttttatcagg gctagttcca aacaaaccgc
      601 tcgatgaaaa tgctttacct cgggcaatta gaagaattca aaaaagagtt ggtattcctg
      661 aatggagtgc acatgattta aggcgaacat ttgcaactca gctgggtgaa acacttcata
      721 ttgatccggt tgttattgaa aaatgtctcg gacataaaat gccacgtatt atggctacct
      781 acaataagaa tgagatgtta cctcaaagaa aggaagcttt agagcgatgg tctaaatata
      841 ttcaagaact tgtcgggcat tcagaagaaa atagctcatc tcaatctaat ttaattttgc
      901 agacttctaa aaacttatgc ttaatttaaa taacataacc gctaatctta gagggcctta
      961 gctattgcgt aaccatagcg gttttttatt tgtaattatg ataacaacaa taaaatactc
     1021 taaatcccct ctttagtttc aagaccagaa agaaaaatta gagagaagag gcttaataat
     1081 ttttaataaa gataaagcat tggcaacatt atcttgaatt agcttttaca gattaagcgt
     1141 aaatcaccca ttatggctta cataaaaaag caggtaattt cctgctttcg ttattatcac
     1201 aaagcttgat tataaccttt tcttatattt gtacttatcc attcgtcaat aacttcttta
     1261 tgccagacaa gcgttctccc atgcagtttt actggtaaag gaaatttacc atcggtccac
     1321 caacggcgca aagttaacct gtcctttccg ataatgactt ctacgtcatt gataaacaaa
     1381 atctgctgtg taaataaatc attaactggt cgcatcatta ctctccaaat ccctttgtta
     1441 ctttaatagc aaacgtgcct tcgattcgcc tttgagttcc atatcaatga aggtgataaa
     1501 tagtttcaac aaattcaatt cgatattact ttctctcggt aaccgattgt aatgatgtat
     1561 caattgcaga tgagcaacag atgagttaga taagtttgca attcgctgta aagaataact
     1621 ggtggaatcg agtaaatact gaataattcc tttgtaaatt acgatcttct gatcatgaaa
     1681 catgcgacat actc
//
LOCUS       DATNSN010000294         1686 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.296, whole genome shotgun sequence.
ACCESSION   DATNSN010000294 DATNSN010000000
VERSION     DATNSN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.296"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..387)
                     /locus_tag="VAH76_003123"
     CDS             complement(<1..387)
                     /locus_tag="VAH76_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO1387695.1"
                     /translation="MIFHQLFDQDSCTYTYLIGSTFSKHAIIIDPVRSQVSHYINLIN
                     KLELNLVASIDTHLHADHITGSGQLTILTDCHSMIGMESKAKFVHIKFHDNEILNFGN
                     IKIKAMHTPGHTPDSYCFVMEDRIFTG"
     gene            599..1618
                     /locus_tag="VAH76_003124"
     CDS             599..1618
                     /locus_tag="VAH76_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO1387696.1"
                     /translation="MIERAFSLLFIQFSIMQIQNTEINIGVIIVNYNSAHCLSKAITA
                     LEKQSYKPYQIILFDNSSIENLPEIVHTTKLPIKIVVSNINLGFAAGNNKAIEYLDPK
                     VNWIALLNPDAYPHKDWLLEMVSAIKTHPQYAFMGSKLICENEPYLLDGTGDVYHISG
                     KAWRLNHRKPLDKAPKDMQELFAPCAAAALYRRDAFNAVKGFDEQYFCYYEDVDLAFR
                     LWLAGYKGCYIPTAIAEHTGSAITKKHSDFYTYYGHRNLVWTYFKNMPLLLLIATLPL
                     HLLLNIATLLLFIKRKQTFVIIKSKIDAIKGLPTLLQQRRHIQKSRVLSNFQLLKILK
                     KGLPW"
BASE COUNT          592 a          282 c          271 g          541 t
ORIGIN      
        1 gccagtaaat attctatctt ccattacaaa acaataggaa tctggagtat gccctggcgt
       61 gtgcatagcc tttattttaa tatttccaaa atttaaaatc tcattatcat gaaatttgat
      121 gtgcacaaat ttcgctttgc tttccattcc tatcatagaa tgacaatcag taaggatagt
      181 taattggcct gatccagtaa tatgatcagc atgtaaatgt gtatcaattg aagcaaccaa
      241 atttaattct aacttgttaa ttagattaat ataatgcgaa acttgagatc gtaccggatc
      301 aataataatt gcatgcttgc taaaagtaga gcctatcagg taagtataag tgcaggaatc
      361 ctgatcaaat agctggtgga aaatcatttt accaatccct ataaaaaaac tttttgttat
      421 tagccatgtt taataacttg aattaattga gtaaaaatat tttatttttt catctgcgca
      481 aaaatgaaaa taaattcaca atatatagca aaaagactgc atatccaata aaaacaaagc
      541 attaatttaa atatcattaa agattaaaaa atgatattct tggcataaac tagagtaaat
      601 gatagaacgc gctttttctt tgctcttcat ccaattttca attatgcaaa ttcaaaatac
      661 tgaaattaat ataggcgtta taattgttaa ttataattca gcacattgcc tctctaaggc
      721 aattactgct ttagagaagc aatcatacaa accgtatcaa ataatactgt ttgataattc
      781 gagtattgaa aatttacccg agatcgtcca cacaactaaa ttaccaatta aaattgttgt
      841 atccaatatt aacctgggat ttgcagctgg gaacaataag gccattgaat atctagatcc
      901 aaaagtcaat tggatagcat tgcttaatcc tgacgcctac ccgcacaagg actggttact
      961 agaaatggtt agtgcaatta aaactcatcc gcaatatgct ttcatggggt caaaactaat
     1021 ttgtgaaaat gagccttatc tattagatgg gacaggcgat gtgtaccata ttagcggaaa
     1081 agcatggaga ttaaaccaca gaaaaccatt agacaaagcc cccaaagata tgcaagaact
     1141 gtttgctccc tgcgctgcag ctgccttgta tagacgtgat gcattcaatg ctgtaaaagg
     1201 gttcgatgag caatattttt gttattatga agatgttgat ttggcttttc gattatggct
     1261 agcaggttat aaaggatgtt atattcctac agctatcgct gagcatacag gctccgccat
     1321 aacaaagaaa catagtgatt tttatactta ttatggccat agaaatctag tttggaccta
     1381 tttcaagaat atgccattgt tattgcttat tgcaacttta cccttacacc ttcttttaaa
     1441 tattgctact cttcttttat tcattaaacg aaagcaaact tttgtaatta taaaatctaa
     1501 aattgatgcg attaaaggtt tgccaacact actacaacaa agaaggcaca ttcaaaaatc
     1561 gagagttctt tctaattttc aattactaaa aatcttgaaa aaaggcttgc catggtagct
     1621 aatttagtaa cagttgttat agtcaattat aattcgggcg aaatgcttgg taagtgcgta
     1681 gagcat
//
LOCUS       DATNSN010000295         1672 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.297, whole genome shotgun sequence.
ACCESSION   DATNSN010000295 DATNSN010000000
VERSION     DATNSN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.297"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(88..612)
                     /locus_tag="VAH76_003125"
     CDS             complement(88..612)
                     /locus_tag="VAH76_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387697.1"
                     /translation="MSLNQLNIILFGMLISQFVLADVPVEVIGSCKNSRAVNASVTYT
                     KIIPPAGVDDSPGCTNHIESTEGSFNYGAITCNHENYLILKSDRINLNTAQNHSVNPS
                     ITPGNRITQLSDWSKIVFDNNEYLCIDVELSQSGEGADVSQYYIVENAFNTSTPVLHF
                     YFFNKDIMPMTDAN"
     gene            complement(603..893)
                     /locus_tag="VAH76_003126"
     CDS             complement(603..893)
                     /locus_tag="VAH76_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387698.1"
                     /translation="MNALDPEGVDYANGGCFWDGNDLVTKGAKHRHYRKGYKFTDPTH
                     DVLSVGDSAPANLTGKFGTYNYTYESTAGYGQTVFWKLTQEFLTAQRGKQCH"
BASE COUNT          531 a          293 c          298 g          550 t
ORIGIN      
        1 gtaatgacga aaacgagtat tgatagaaac taatttttaa gtttttcaca ttcaaacgaa
       61 tcaatacact taccaaaaat ttctcattta gttagcatca gtcattggca taatgtcttt
      121 attgaaaaaa taaaaatgga gaactggtgt agaagtatta aaagcatttt caactatata
      181 atattgtgaa acatcagcgc cttctccaga ttgtgataat tcaacatcaa tacaaagata
      241 ctcattatta tcgaatacta tctttgacca atctgataat tgtgttatac ggtttccagg
      301 agttatcgag ggattgactg aatgattttg agctgtattt aaattgatgc gatctgactt
      361 aagaatcaga taattttcat gattacatgt tattgcaccg taattaaaag atccttctgt
      421 ggattctatg tgattggtac atccggggga atcatcaact cctgctggcg gaattatttt
      481 ggtataagta actgaagcgt taactgctct agaattttta caagatccaa tcacttcaac
      541 aggtacatct gccaatacga attgagaaat tagcattcca aataaaataa tatttaattg
      601 atttagtgac attgtttacc cctttgcgcc gttaagaatt cttgtgttaa cttccaaaat
      661 acagtctgac catatcctgc agttgattca taagtgtaat tataggtacc aaatttacca
      721 gttaaatttg ctggggcgct atctccaact gacagtacat catgtgtagg atctgtaaat
      781 ttataccctt tgcgataatg tctatgtttt gcccctttag ttactaaatc attaccatcc
      841 caaaagcatc caccatttgc atagtcgaca ccctcaggat caagagcatt cattgcagca
      901 ttatatagat ctggatattc gatagctaca tcagaacaca tcacttgacg atatcttact
      961 actttattag aggctgcagc tgaataagct gggttatcca gaattaattg attcacagta
     1021 ggatagccat tagaattttt atatctaact acagcatttg caattgcagc tttttcctca
     1081 taggtgctat tagtagcaga tgcttcccca taaactatag ctgcgagtaa ttttacttca
     1141 gaaaccgtag ctggagtttc agtacaagtt gaatttggtc ttgagcagct taatccatcg
     1201 ttagaacgag tccaggtagg attagggcaa gtggtaaaag attcactgca gagccaccaa
     1261 ggtgtattgt agtatgctaa tgttgaatcg gcgcagtacc aagtattagc tcccataagt
     1321 acgttatctg ttggggagta acatgcaggg tcggcgcttt tgtgtggaac aattttccat
     1381 gtatatccgt atgatgctaa tttactacag gcctcttcat aattagaacc gtgcacaacc
     1441 tcaggacctg ccgtaataat ataagccaag tcatttttat aatttataac ctgtattgac
     1501 gggatagttt cagcaggttt tgcaaatgct attgagctga taataaataa acttaagcat
     1561 aatatttttg atatcctcat aaacatcctt gttaatgaat ttttcctatt agcataaagg
     1621 atctagtatt ggtattacaa ttcttagctt aagaaatagg gaagggaatt aa
//
LOCUS       DATNSN010000296         1641 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.298, whole genome shotgun sequence.
ACCESSION   DATNSN010000296 DATNSN010000000
VERSION     DATNSN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.298"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..457
                     /locus_tag="VAH76_003127"
     CDS             <1..457
                     /locus_tag="VAH76_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO1387699.1"
                     /translation="KLDTENTPLTAENFLNYVRKGFYNDTIFHRVIDGFMIQGGGLNT
                     NMEQKTTASPIQNEAKGAKPNKRGTIAMARTMDPHSATAQFFINVADNGFLNYSGDHP
                     QGYGYCVFGEVVEGMDVVDAIAKVKTGQRNGHADVPVEEISIIEVRELA"
     gene            complement(667..1296)
                     /locus_tag="VAH76_003128"
     CDS             complement(667..1296)
                     /locus_tag="VAH76_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO1387700.1"
                     /translation="MLLHYLFIVGICVEAITGALAAGRKKMDFFGVLLIASITALGGG
                     TVRDTLFNTYPLTWVAHPEYLLYTSVCAFLTIFIAHSLVRIMKVFLILDALGLSTFVI
                     IGTQKVLAHGMPPSVAVISGMITGICGGMLRDILCNDIPLVLRKELYAVIALAGALLF
                     ITLDYFHIPHNLNIIITLLCIFTARLLAIFFHIEIPIFDYSESIKRRKK"
     gene            1413..>1641
                     /locus_tag="VAH76_003129"
     CDS             1413..>1641
                     /locus_tag="VAH76_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387701.1"
                     /translation="MNNTIDNQSQEELTHFDANTPKSIIQLDRKLREMKSEVEMKLND
                     PSSYNGDDKEVYFQKLSKLSEEINQAIKSLDT"
BASE COUNT          592 a          287 c          311 g          451 t
ORIGIN      
        1 caaattagat acagaaaata ccccattaac tgcagaaaac ttcctaaatt atgtgcgtaa
       61 aggtttttat aatgacacga ttttccatcg tgttatcgat ggttttatga ttcaaggcgg
      121 tggtttgaat acaaatatgg agcaaaaaac aactgcctct ccaattcaaa atgaagcaaa
      181 aggagcgaaa cctaacaaac gtggaaccat cgcaatggcg agaacaatgg atccccattc
      241 tgcaacagcc caatttttta ttaatgttgc ggataatggt tttctgaatt acagtggcga
      301 ccaccctcaa ggttacggtt actgcgtatt tggagaagta gttgaaggga tggatgtggt
      361 tgatgctatt gctaaagtga aaacaggcca acgaaatggt catgcagatg tccctgtaga
      421 agaaataagc atcattgagg ttagagaact ggcttagcga gaaaatatat ggctgaaata
      481 gcataggttt tccatggcaa aagcatattt tctttaattt catccccagt atggaagagg
      541 gatcttcctt atgcatgcag gaagccgcat ttgcaaaaca gcgtgccttt tttcaggggc
      601 gaaatatcat caggtatctc tcagggatta acatcattat ttaagtattt taatgaaaaa
      661 atgacgttat ttttttctcc tctttatact ctctgagtaa tcaaaaattg gaatttcaat
      721 atgaaaaaaa atagccaata agcgtgcggt aaaaatgcat aacaaagtga taatgatatt
      781 taaattatga gggatatgaa aatagtctaa tgttataaac agaagcgcac cagccagggc
      841 aataacagca tataattctt ttcttaaaac cagaggaatg tcattacata aaatatctcg
      901 caacattccc ccgcaaatcc cggtaatcat tccactgatt actgcaacac ttggtggcat
      961 accatgagcg agaacttttt gtgtaccaat aataacaaaa gttgagagac caagggcatc
     1021 aagaattaag aatactttca ttattctaac cagtgaatga gcaataaaaa tagttagaaa
     1081 agcgcatact gaggtatata aaagatattc cgggtgagcc acccaggtca gtggataagt
     1141 gttaaataat gtatccctta cggtccctcc tcccagcgca gtgattgacg caataagtag
     1201 caccccaaaa aaatccattt ttttacgtcc tgctgccagg gctcctgtaa tcgcttctac
     1261 acaaatcccc acaataaaaa gataatgaag taacatatga tttgctgcta tttaaaaaga
     1321 caatgatagc actaatagta caaatgaaga aatgctgtac ttcgactaga atttaatagt
     1381 aaggttctaa ttattttggc aggagcaaat tgatgaataa tacgatcgat aatcaatcgc
     1441 aagaagaatt aactcatttt gatgcaaata cgcccaagag catcattcaa ttggatagaa
     1501 agctcaggga aatgaaaagc gaagtcgaga tgaaactaaa tgatccttca tcttataatg
     1561 gggatgataa agaagtttat tttcaaaaat tatcaaaatt atctgaagaa ataaatcaag
     1621 caatcaagag cttggacact t
//
LOCUS       DATNSN010000297         1631 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.299, whole genome shotgun sequence.
ACCESSION   DATNSN010000297 DATNSN010000000
VERSION     DATNSN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.299"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            67..>1631
                     /locus_tag="VAH76_003130"
     CDS             67..>1631
                     /locus_tag="VAH76_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEO1387702.1"
                     /translation="MLAESVIGIVRAVNGLLEKVNVQGQASLVKSGARLEEGDVLTLL
                     SGEAYIQFINGFPEALALEKPVKLDGVSPTLQYGAEDLKEQMVQEAIAKGIDPSVILD
                     VLGSAAAGAEAVGSGGDAFIMDPLFGFGHVTAGYPTGPISFAYEADTQQLFWFVPEET
                     GAIAESELTQEPESIPQIPQFTTNQAVLTVFEDALPSGIADSAGQARIASSSLSSLLT
                     SSADVAASFAFNSNLSALPTLKSGGIDLSYELSADKRTLTLRESSTQGSGAEVMKLEL
                     TADGQLTQTLMNSIDHPAADSDDGEWMRFDLSPLIDVTFTRTSNGTVLESRTLPANAV
                     VAGIQDDVPIARAQLTNNEILLDETIGMKVGDADAANDDFNPTTTVDPFNNIYGTPLG
                     LVQNANLLDASTSEMGGDYKNATMTHLLKITDAVSGLQTTDGTPINLFLESNGDITGR
                     AGDVGAPAVFAIRMNPNTGAISVAQYGPIKQFDTNSHDEAVDLTGRISAVVTAKDSDG
                     DESNAEIPIGQLI"
BASE COUNT          497 a          313 c          371 g          450 t
ORIGIN      
        1 actacaagga ggcttaatcc ttgaactatt attaatatga ataatagtaa actttgagga
       61 cagaacatgt tagctgaatc tgttatcggt atagtaagag ccgtcaatgg actactagaa
      121 aaagtaaatg tgcaaggaca agcaagctta gtaaagtctg gcgctcgatt agaggaaggg
      181 gacgtcctga ctttattaag tggtgaggct tacatacaat ttattaatgg atttcctgaa
      241 gcattagcct tagaaaaacc ggtcaagcta gatggtgttt ccccaacatt acagtatggt
      301 gcggaagatt taaaagaaca aatggtgcaa gaagcgatag ccaaaggtat tgatccttca
      361 gtcatacttg atgttcttgg ctcagcagca gcaggtgccg aggcggtggg atcaggaggc
      421 gatgcattta ttatggatcc tttgtttggc ttcggacatg tcacagcagg atatcccacc
      481 ggacctattt cctttgctta tgaggcagat actcagcaat tattttggtt tgtgccagaa
      541 gagacagggg cgattgcaga atccgaattg actcaagagc ctgaatctat cccgcaaatt
      601 cctcaattca ccacaaatca agcagtgcta acagtttttg aggatgcttt gccaagtggt
      661 attgctgatt cagcaggaca agcaagaata gcaagttctt ctttaagttc tttgctcacc
      721 tcttcagcag acgttgctgc ttcctttgct tttaatagca atttaagtgc tcttcctaca
      781 ttaaaatcag gtggtataga ccttagttat gagttatccg ctgataaacg taccttaacc
      841 ttaagagaat caagcactca agggtcaggg gcagaggtca tgaaacttga gctgacagca
      901 gatgggcagt taactcaaac attaatgaat tcaattgatc acccagcagc tgatagcgat
      961 gacggcgaat ggatgagatt cgacctttca cctttaattg atgtgacttt cacaagaaca
     1021 agtaatggca cggttttaga gtcaaggaca ctgcctgcta atgcagttgt tgcaggaatt
     1081 caagacgatg ttccaatagc aagggctcaa ttgaccaata atgaaatctt gctggatgaa
     1141 accataggaa tgaaagtcgg tgatgctgat gcagctaatg atgattttaa tccaacgact
     1201 actgtggatc cttttaataa tatttatggt acacccttag gtttggtaca aaatgcgaat
     1261 ctcctcgatg ccagtacttc tgaaatgggg ggggattata aaaatgcaac aatgacccat
     1321 ttgttaaaaa ttaccgatgc tgtttcagga ttacagacaa ctgacggcac tcctatcaat
     1381 ctctttttgg aatcgaatgg tgatattacg gggcgagcag gagatgttgg tgctcctgca
     1441 gtttttgcaa ttcgcatgaa tccaaatact ggtgcaatta gcgttgcaca gtatggccca
     1501 attaaacaat ttgataccaa cagccatgat gaagcagttg atttaacagg acgcatttca
     1561 gccgtcgtta ccgccaaaga cagcgatggt gacgaatcta acgcagaaat accaatagga
     1621 caactgatta t
//
LOCUS       DATNSN010000298         1607 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.300, whole genome shotgun sequence.
ACCESSION   DATNSN010000298 DATNSN010000000
VERSION     DATNSN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.300"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1607
                     /locus_tag="VAH76_003131"
     CDS             <1..>1607
                     /locus_tag="VAH76_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1387703.1"
                     /translation="GNIEFCNQQFLKMWGISPGFIDSHNDTETIQYVLNQLKNPEEFL
                     KQIKELYNNPKQESFDELHFKDGKIFERYSQPHWLDNKIVGRVWSYRDVTLRTRLAYQ
                     ANHDSLTGLPNRTALYDRLEHAINYCKRLNTSLSVFFLDIDNFKLINDSLGHDAGDIL
                     LYEFSRRLKGCIRHSDTVARFGGDEFVLLLITTEPKDVILVAQNILKAVAQPIKLPTQ
                     EVIVTTSIGISLFPKDGIDANTLLKNSDTAMYLAKKEGRNNFQFYNNSINQQSLKRLE
                     IQTQIHNAIKNNEFFILYQPIYNLRNGELVGAEALIRWGHPAIGLLSPDEFIPIAEET
                     GLINQVGEWVLRNACIQNKTWQLMGLPHISISVNVSWRQLKNSNFIEIAESVLKESRL
                     DPQYLEIEFTESTMIQKSTPIDSMLVKISQLGIKMAIDDFGTGYSNLSYLKTFPFNKL
                     KIDRSFIQNCTTNPNDASIVEAIIAMAHSLELKVLAEGVDTIDKAFFMHQHGCDEVQG
                     FLYGLPVKADQFVKLMQKNRIRHSKFI"
BASE COUNT          537 a          286 c          297 g          487 t
ORIGIN      
        1 ggtaatattg aattctgcaa tcagcaattt ctcaagatgt gggggatttc tccagggttt
       61 attgacagtc ataatgacac agaaacaatt caatatgtat tgaatcagct taaaaatccg
      121 gaagaatttt taaaacaaat taaagaacta tacaacaatc caaagcagga aagctttgat
      181 gaattgcatt tcaaggacgg taaaatcttt gagagatact cacaaccaca ttggttagac
      241 aataaaattg taggacgtgt ttggagttat agagatgtga ccttaagaac cagattagcc
      301 tatcaagcca atcatgattc tctaactggt ttacccaatc gtacagctct ttacgatcgc
      361 ctggagcacg cgattaatta ctgtaaacga ttaaacacct cattatcggt attttttctg
      421 gatattgata attttaaatt aattaatgac agcctgggcc atgatgccgg tgatatatta
      481 ttgtatgaat tttccaggcg tcttaaagga tgcatacgtc atagcgatac agttgctcga
      541 tttggcggtg atgagtttgt tttgttattg ataaccactg aacctaaaga tgttatttta
      601 gtagcgcaaa acattttaaa ggctgtagcg cagcctatca aattgcctac ccaggaagtc
      661 atagtaacga caagtatagg aattagcctt ttccctaaag atggcattga tgcaaacacc
      721 ttactcaaaa attccgatac tgcaatgtac ctggctaaaa aggaagggcg caataatttt
      781 caattttata ataattcgat caatcaacaa tcgctcaaac gccttgagat acaaacacag
      841 atacacaatg caattaaaaa taatgaattt tttattcttt accagcccat ttataattta
      901 cgtaacggtg aattagtagg tgccgaagcc ctgatacgtt gggggcatcc tgcaataggg
      961 ctcctctctc cggatgaatt tattcctatt gctgaagaaa ccgggttgat taaccaggtt
     1021 ggcgagtggg tattaagaaa tgcctgtatt caaaataaaa cctggcagct tatggggtta
     1081 ccgcatatta gtatttccgt taacgtctct tggagacagt taaaaaatag caattttatc
     1141 gagattgcag aatctgtatt aaaagaatcc agactagatc ctcagtattt ggaaattgaa
     1201 ttcaccgaaa gcactatgat acaaaaatca acgcccattg attcgatgtt agtaaaaatt
     1261 tcccagttag gtattaaaat ggcaattgat gattttggta cgggttactc aaacttaagt
     1321 tatttaaaga ctttcccctt caataaactt aaaattgatc gttcatttat tcaaaactgc
     1381 accactaacc caaatgatgc ttccatagtt gaagcaatta ttgccatggc ccatagcctc
     1441 gaactcaaag tattggcaga gggagttgac acgattgata aagcgttttt tatgcatcaa
     1501 catggctgtg atgaagtaca aggtttcctt tatggcttac ctgttaaagc tgatcaattt
     1561 gtgaaattaa tgcaaaaaaa tagaatccgc cattcaaaat ttattaa
//
LOCUS       DATNSN010000299         1603 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.301, whole genome shotgun sequence.
ACCESSION   DATNSN010000299 DATNSN010000000
VERSION     DATNSN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.301"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1603
                     /locus_tag="VAH76_003132"
     CDS             <1..>1603
                     /locus_tag="VAH76_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO1387704.1"
                     /translation="DDSWFIEKKGFSFADAILVITAVINFQNEKLTQTLSELKDKDVN
                     DWSVLDGYIFSANDITQESNLTPEVVSNVLDSFCLPQEGSNDSFNELGDFNLTNAYPL
                     IFLNDSNYLLLQHYTLLEALYETPFFWLKDDEAYKDEAATNRGAFVENVAVEWLSRVF
                     GKHNVFKNVEIKASKKEKSGEIDVLVLYADRAIILQAKSKKLTLAARKGNDNLLKDDF
                     KKAIQDAYDQGFSCAELLNNKQHKFYINGTDEIEVPDNLKKIYIFCTISEHYPSLSYQ
                     SKLFLNFKETEQIAAPFVMDLFLLDVMAEMLNSPLYFLSYVDRRCKNLEKFGASHELL
                     ILDHHLKRNLWLDGDNTFMYVADHDCLELYEALLTRRENYPGKETPEGILTRFKNTSF
                     EKLIKQITHLEHPEAVNLGLILLTCSGEFINGFNSDINRLLKLVKTDGNGHDITILLY
                     NNCGLTIHINFDSEFIAQKVLKSHCERRKYIAKATDWYGLCLNPVNLDIRLALSLSYD
                     WKYSEEMEKTVKPLIANQEKVAKSGK"
BASE COUNT          555 a          232 c          298 g          518 t
ORIGIN      
        1 agatgattcg tggtttattg aaaaaaaagg gttttctttt gcagatgcaa ttctagttat
       61 aacagctgtc attaactttc aaaatgaaaa acttacccaa acgttatcag aattaaaaga
      121 caaggacgtg aatgattggt cagttttaga tggttatatt ttttcagcga atgatattac
      181 ccaagaatca aatttgactc cggaggtggt ttctaacgta ttagattctt tttgtttgcc
      241 tcaagaggga tctaatgaca gtttcaatga actgggggat tttaatttaa ccaatgcata
      301 tcctttaatt tttctcaatg attccaatta cctactatta cagcattaca cactgctaga
      361 agcgctgtat gaaacgcctt ttttctggtt aaaagatgat gaggcttata aagatgaagc
      421 cgcaacaaat agaggtgcgt ttgttgaaaa cgtggcggta gaatggttat ctcgtgtttt
      481 tggcaaacat aatgttttta aaaatgttga aattaaggca tcaaagaaag aaaagtcagg
      541 ggaaatagat gtacttgtac tttatgctga tagagcaatt attttgcagg caaaatcaaa
      601 aaaattaacc ttagcagcac gcaaaggtaa tgataatctt cttaaagacg attttaaaaa
      661 ggctatacaa gatgcctatg atcaagggtt tagttgtgct gaactattga ataacaaaca
      721 acataaattc tatattaatg ggacagatga aatcgaagtc cctgataatc taaaaaaaat
      781 atatattttt tgtacgattt cagagcatta tccaagccta agttatcaat caaagctctt
      841 cctaaatttt aaagaaaccg agcaaatcgc tgcaccattt gttatggatt tattccttct
      901 tgatgtaatg gcagaaatgt taaattctcc attgtatttt ctaagttatg tcgatcgtag
      961 gtgtaagaac ctagaaaaat ttggtgcatc tcatgagtta ttgatcttgg atcatcattt
     1021 aaaaagaaac ctatggcttg atggtgataa cacatttatg tatgttgctg atcacgattg
     1081 cttagaactt tatgaggctt tattaactag gcgtgaaaat tatcctggaa aagaaacccc
     1141 agaaggcata ttaacaagat tcaaaaatac ttcctttgaa aaactaataa agcaaattac
     1201 gcatttagaa catccagaag cggttaacct aggtttaatt ttgctaacgt gtagcggtga
     1261 gtttattaat gggtttaata gtgatatcaa tcgccttctg aagttagtta aaactgatgg
     1321 gaatggtcat gatattacta tattacttta taataattgt ggacttacaa tacacattaa
     1381 ttttgattcc gagtttatcg cacagaaagt tcttaaaagt cattgtgaga ggcgaaagta
     1441 cattgccaaa gccactgatt ggtatggtct ttgtctaaac cctgttaatt tagatattcg
     1501 attagcacta agtttaagct atgattggaa atattcagag gaaatggaaa aaactgttaa
     1561 accattgatt gctaatcagg aaaaggtagc taaatcaggc aaa
//
LOCUS       DATNSN010000300         1560 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.302, whole genome shotgun sequence.
ACCESSION   DATNSN010000300 DATNSN010000000
VERSION     DATNSN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.302"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..897
                     /locus_tag="VAH76_003133"
     CDS             <1..897
                     /locus_tag="VAH76_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387705.1"
                     /translation="SCLKGFITTVDCKSFSKAAQKLYISPSKLSKQITWLEEELKVTL
                     LIRSTTRLTVTDSGHRLYEKALQLFEQLEAVKVIATPDQLELNGVIRLYLTVTPAIPY
                     LTKLSIQFMRQYPKIELDIIVGSDTKELYSSTFDLAISFDDVKHSKLVCKRLFSIQRK
                     VFASPGYIAQHGLPQTLDELPKHNCLINTLYGLQNKWVFNKKIIHVSGNFKSNNAGVL
                     KQAAVADVGILWAPYFSVYEEIKNEALIPVLPNESSPDINLYAICPKPTVDEQKINLL
                     LNFFCEKALSENIATSFIDGNN"
     gene            complement(1252..>1560)
                     /locus_tag="VAH76_003134"
     CDS             complement(1252..>1560)
                     /locus_tag="VAH76_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEO1387706.1"
                     /translation="SDKPGRYQASGAAHGAFSQESDPREIQIERFSKEIAKTLEHGSA
                     VNAYEKLILASAPHMHGLLLKNINRQVEKSIIHVIPKDLIHFSENDLLDYVNNELKPK
                     "
BASE COUNT          499 a          295 c          266 g          500 t
ORIGIN      
        1 tcctgtttga aaggctttat tacaaccgtt gattgcaaaa gcttttcaaa agcagcacag
       61 aaattgtaca tatccccctc taaattgagc aaacaaataa cctggctgga ggaggagtta
      121 aaagttacct tattgatacg ctctacgaca cgattaactg taactgattc aggtcacagg
      181 ttgtatgaaa aggcattgca gcttttcgag caattagagg cagtcaaagt cattgcaaca
      241 cctgatcagc tcgaattaaa tggcgtaatc aggctctatc ttactgtcac tccggcaatt
      301 ccctatttaa ccaaattaag tattcaattt atgcgacaat accccaagat tgaactagat
      361 attattgtcg gttcggatac aaaagagctt tactcctcta cctttgatct ggctatttca
      421 tttgatgatg tcaaacattc aaaactggtt tgcaaaagat tattttcaat tcaaagaaag
      481 gtctttgcct cacctggtta tattgctcaa catggacttc ctcagacact cgacgaatta
      541 ccaaagcata attgtttgat taatactctc tatggcctgc aaaataaatg ggtttttaac
      601 aaaaaaatca ttcatgtctc gggaaatttt aaaagcaaca atgctggtgt cttaaaacag
      661 gctgcagtag cagacgtggg gattttgtgg gcaccttatt tttcagttta tgaagaaata
      721 aaaaatgaag cattaattcc agtattgccc aatgaatctt caccagatat caacctttat
      781 gcaatttgcc ccaagcccac tgtagatgaa caaaaaatta atttactttt gaactttttt
      841 tgtgaaaagg ccttgtcaga aaacattgcg acatccttta ttgatggaaa taattaaaga
      901 tggattagaa cttttgactt tgatactcag aaaatacaca agaatgattg tattacccta
      961 aaacttcagc agacgcatgg aatttagggt tgtgtgatga atctttaatg agttagtacc
     1021 atttgacaat caaaattcaa gccatgtcct tcaatacttg aggaagcaag gataaattag
     1081 acactgttca accgtttatt aggcaaagcc caaaataatg tcttatccac tctgattctg
     1141 atagaacgta ttgttaccat actgaaacca tttaagagca gaaaatcctg tgtcctattt
     1201 atttaacacg tatcaaattt actcaaaact cacttgttaa ccggcttcct gttactttgg
     1261 cttcaattca ttatttacat aatctaaaag atcattttca gagaaatgga ttaaatcttt
     1321 aggtattaca tgaattatcg atttttcaac ttgcctgttg atgttcttta atagaagacc
     1381 atgcatatgc ggagcagaag ctaatatcaa tttttcatat gcattcacgg cgctgccatg
     1441 ttcaagtgtt ttggcaattt cctttgaaaa tctctcgatt tgtatttctc tggggtctga
     1501 ttcttgactg aatgcaccat gagcagcccc tgaagcttga tatcgacctg gtttatcaga
//
LOCUS       DATNSN010000301         1560 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.303, whole genome shotgun sequence.
ACCESSION   DATNSN010000301 DATNSN010000000
VERSION     DATNSN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.303"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..552)
                     /locus_tag="VAH76_003135"
     CDS             complement(<1..552)
                     /locus_tag="VAH76_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO1387707.1"
                     /translation="MAKILITGATGFIGRSLVPALLSEGHDVRCAVLQLDSTLQAEQI
                     VINNLEVHTDWTDALRNVEIVIHLAARVHIMKEYATSSLDEYCKINSIATKNFVEQAA
                     QNNVKRFIFLSTIKVHGEFSQNNLPFSEDCRTQPEDPYAKSKLYAEQFIQEICQNARM
                     EFVILRPPLVYGPYVKANFLRILQ"
     gene            complement(573..1448)
                     /gene="rfbA"
                     /locus_tag="VAH76_003136"
     CDS             complement(573..1448)
                     /gene="rfbA"
                     /locus_tag="VAH76_003136"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO1387708.1"
                     /translation="MKGIILAGGFGTRLYPLTKSISKHMIPVYDKPMIYYAISTLMLA
                     NIREILIISTEEHLPLYQNLLSDGSQWGISFAYLVQNEPRGIAEAFILGEKFIGNDSV
                     CLVLGDNIHQGRGFSELLQNAKSKLNGATVFAYYVDKPQAYGVVEFNEKQQVLSIVEK
                     PAQPKSNYAVTGLYFYDNQVIEIAKSLKPSGRGELEITDINQFYLYENKLDVQILGRG
                     FVWLDMGTPETLLSASNYICTIEQRQGLKIGCPEEIAWRMKFISDDELLCRARELNKS
                     SYGQYLAELLNHKRY"
BASE COUNT          486 a          322 c          243 g          509 t
ORIGIN      
        1 ttgtaatatc ctgagaaaat tagccttcac ataaggtcca tagaccaacg gtggtcttaa
       61 tataacaaac tccatccggg cattctggca aatttcttgt ataaattgtt cagcatacaa
      121 cttacttttt gcatagggat cttcaggttg agtcctacaa tcttcagaaa atggcagatt
      181 attttgtgaa aattcgccat gtacttttat ggtactaaga aatataaatc gctttacatt
      241 attttgagct gcctgctcaa caaaattttt tgtagcaata ctattaattt tgcaatattc
      301 gtctaaagag gatgttgcat attctttcat tatgtgaact ctggcagcta aatgaatgac
      361 tatttccaca ttccgcaaag catcagtcca atcagtgtga acctcaagat tgtttattac
      421 tatttgttct gcttgtaatg tactatccag ctgcaaaaca gcacaacgca cgtcatgtcc
      481 ttcagaaagc aaggcaggaa ctaaacttcg tcctataaat cctgtcgcgc cggtaattaa
      541 tatttttgcc actactttat aacctgaaaa aattaatatc gtttatgatt aagcaattct
      601 gcaagatatt gaccgtaact acttttatta agctctctag ccctgcacag taactcatca
      661 tcactaataa acttcatacg ccacgcgatt tcttcagggc atccaatttt taaaccttgc
      721 cgctgttcga tagtacagat atagttactt gctgataaca gggtctctgg tgtacccata
      781 tccagccaga caaaacctct tcccaaaatc tggacatcca gtttgttttc atataaataa
      841 aactgattga tgtcagttat ttccagctcc ccccttccag agggttttaa actcttggca
      901 atttctataa cctgattatc ataaaaatat aatccagtta ctgcgtagtt ggatttgggc
      961 tgtgctggtt tttccactat ggataggact tgttgttttt cattgaactc caccacacca
     1021 taggcctgag gtttatcgac ataataggca aaaactgttg caccgtttaa ttttgatttt
     1081 gcattttgta ataattcaga aaaacctctg ccctggtgga tattatcccc caaaaccagg
     1141 caaacagaat cattgccaat aaatttttca cctaaaatga atgcttccgc gattcccctt
     1201 ggttcatttt gcacaagata ggcaaatgag atgccccact gagaaccatc actcaataaa
     1261 ttttgataca gcggaagatg ctcctccgtt gaaataatca gtatttccct gatattagcc
     1321 aacattaatg tagagatggc ataataaatc attggtttat cataaaccgg aatcatatgc
     1381 ttactaatgg attttgtcag aggatacagt cttgtaccaa aaccacctgc caggataata
     1441 cctttcatta tatcgagcct actctctatt tttctttgaa caaataacag ccttaaatat
     1501 tatcaagttt attgaagtaa tacattaaaa tacgttgata atgtcaataa aatataaagt
//
LOCUS       DATNSN010000302         1555 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.304, whole genome shotgun sequence.
ACCESSION   DATNSN010000302 DATNSN010000000
VERSION     DATNSN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.304"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(518..1495)
                     /locus_tag="VAH76_003137"
     CDS             complement(518..1495)
                     /locus_tag="VAH76_003137"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO1387709.1"
                     /translation="MPHYNKPYLTFEQQLELLKSRGLGVTNDKVALEYLGRLGYYRLS
                     GYWYPCRKLLPLVEQQSKSIRPRRSDEFLPGSRFQDVVDLYVFDKKLRLLILDAIERV
                     EVAFRVDIAYLLGERDPFAYTNPDLLHGQFTKIIDPNTGKTKYQEWISKHEQLINRSK
                     EDFVEHYKAKYGLPLPIWVAIELWDFGLLSVFYKGMAVKDKAIIANKYDIPDWQIMES
                     WLRSLNYVRNVAAHHSRLWNRNLIDQPKLAKPGSMLAFDSFIDNVHMTSRVYVVLCIL
                     AHYMKIICPRSTWQMRLVELIHSFPNSNYVSIQDMGFPAEWEQHLFWKN"
BASE COUNT          537 a          327 c          230 g          461 t
ORIGIN      
        1 catactacaa ctgataaaac aatcattcac cttaaataaa agcttactaa aaaacagtta
       61 attactatta ttttttatga atgctgtttg cccccttcaa ttaaatttat gcgcataact
      121 acttatacca attctcaaag atacagctag cactaaatta ggataaattc aatgcagtat
      181 gtgaatctgt ttcacgatgt caccgattga aaaagataaa tcggtgacaa tttgttacca
      241 attaaaaatt acttcatata cgttcaatta ataacaactg caaaaaacct ttgacaaaga
      301 aactgattta aataaacatc atcaatagat gaccagtaaa ttaaactttc accacaattt
      361 tttaactccc caaatcaatg agcctaaatc cgtccaaaac aaggccgacc ctaacccgac
      421 acatcaaccc ggcacaaacc cgacatagaa agttggcttg agaattcaca taagtagtta
      481 aagccgccta agtctatatg cactccacaa aatcaattca atttttccag aataaatgtt
      541 gttcccattc agctggaaat cccatatctt gaatacttac ataattggaa tttggaaagg
      601 agtgtatcag ctccactaga cgcatttgcc atgttgatcg cgggcaaatt attttcatgt
      661 aatgcgccaa tatacataaa accacatata ctctcgatgt catatgaaca ttatcaatga
      721 atgaatcgaa agcaagcatt gaacctggct ttgcaagctt cggctgatca attaagtttc
      781 tattccataa acggctgtgg tgcgccgcta catttcggac ataatttaaa gaacgtaacc
      841 aactctccat aatctgccag tcaggaatat catatttgtt agcaatgatt gctttatctt
      901 taactgccat tcctttataa aatacagaca aaagaccaaa gtcccataac tcaatagcaa
      961 cccatatcgg caatggtaaa ccgtactttg ccttgtagtg ctctacaaaa tcttcttttg
     1021 agcgatttat taattgctca tgtttagaaa tccattcttg atacttcgtt ttacccgtat
     1081 ttggatcaat tattttagta aattgaccat gtaataaatc aggattggtg taggcaaatg
     1141 gatctctttc accaagcaaa taagctatgt ccactctgaa agcaacttct actctctcaa
     1201 ttgcatccaa tattaatagg cgcagctttt tatcaaagac atacaaatcc acgacatcct
     1261 gaaatcgaga acctggcaaa aactcatctg aacgccgagg tcttatagat tttgattgtt
     1321 gttctactaa agggagtaat ttgcggcagg gataccaata accacttaaa cgatagtagc
     1381 ccaatcgacc tagatactcc aatgccactt tatcattggt tacccccaag ccacgagatt
     1441 ttaataattc aagttgctgt tcaaaggtaa gataaggttt attataatgc ggcattttta
     1501 agctttataa aaaagaaaac ccgctcgtgc atttttcaat gtagaggagc aggcc
//
LOCUS       DATNSN010000303         1550 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.305, whole genome shotgun sequence.
ACCESSION   DATNSN010000303 DATNSN010000000
VERSION     DATNSN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.305"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            59..1030
                     /locus_tag="VAH76_003138"
     CDS             59..1030
                     /locus_tag="VAH76_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387710.1"
                     /translation="MYTERSIFKLGKEKSGAIQIGKRGLEQHLLEADICQPFYIKELL
                     SKQDWSIFEEPNKKKKRKPYPAQSMVGLILYGLMQGLSSLRDLERLARVDLGCILITD
                     GIFPDFSSIGYFIRRHAEVLNEAFFIQLTRSILKVTRSGVRSLAGDGTIIEATSSRYT
                     LMRHEAIEKKREALKAQLAAHPEEQNLGAAAEKIEQASTIMQGRIEKRKEQRKPIDTV
                     TINPTEPEAVVQPTKKGKTAVPAYKPSVLANKARVVVALDVHPSNEIDSLVSMLDMTN
                     RANYQEKVEELLLDGGYESHGVLEETLKRDISVLCSSSDNSKKKHKK"
     gene            1027..1512
                     /locus_tag="VAH76_003139"
     CDS             1027..1512
                     /locus_tag="VAH76_003139"
                     /inference="COORDINATES: protein motif:HMM:NF025126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO1387711.1"
                     /translation="MAKKIQKKEFVYHERTDAYTCPQGQQLFPLRRGAASATAPAYTT
                     YATKACQDCPLKKKCTSSKMGRQIKRYKGDELKEALALVMQQPQAQKRLRQRKAMVEP
                     VFSHIRIRQNFNRFKRRGLHGVKVEFTLQIMAYNISRAIARYFSFLIRLIINHIGNFV
                     F"
BASE COUNT          506 a          291 c          339 g          414 t
ORIGIN      
        1 tgctcttgat atagattgaa acctgattaa gatagtgatt ttttgaaaag gatttaaggt
       61 gtatacggag cgttctattt ttaaactagg caaagaaaaa tcaggggcta ttcaaattgg
      121 gaaacgaggg cttgaacaac atttgctaga agcagatata tgtcagcctt tttatataaa
      181 agaattgtta tctaaacagg attggagcat ttttgaagag ccgaataaaa agaaaaaaag
      241 gaagccctat cctgctcaat cgatggtagg attgatttta tatggtctta tgcaaggttt
      301 atcttcctta cgagatttag agcgtttagc gcgagtagac ttgggctgca ttctgataac
      361 ggatggaatt ttccctgatt tttcaagtat tggttatttt attcgtcgcc acgcagaggt
      421 tttaaacgaa gcctttttta tccagttaac ccgctctatt ttgaaagtga ctcgttcagg
      481 ggtccgttca ttagcggggg atggaacgat tattgaggcg acatcctccc gttatacatt
      541 aatgagacac gaggctattg agaagaaacg agaggcatta aaagcccaat tggctgctca
      601 tcctgaagaa caaaacttag gcgcggctgc ggaaaaaata gaacaggcct cgactatcat
      661 gcaaggtcga attgagaaaa ggaaagaaca gcgtaagcct attgatacgg taaccattaa
      721 tccaacagag ccagaggcgg tggtgcaacc gaccaaaaaa ggtaagaccg ctgttcctgc
      781 ttataaacct tccgtgctgg ccaacaaggc cagggtggtt gtggctttgg atgtccatcc
      841 atcgaatgaa atcgattccc ttgtctcgat gctggacatg actaatcgag ctaattacca
      901 agaaaaggtt gaagagttac tgttagatgg aggctatgaa tctcatggtg ttcttgaaga
      961 aaccctgaag cgtgatatca gtgtattatg ctcttcgagt gataacagta aaaaaaaaca
     1021 caaaaaatag ctaaaaaaat tcaaaaaaaa gagtttgttt accatgagag gacagatgct
     1081 tacacctgtc ctcaagggca acaactcttc cctttaaggc gaggagctgc aagtgcaaca
     1141 gctcctgctt atacaaccta tgcaactaaa gcatgccagg attgtccttt aaagaaaaaa
     1201 tgcacatcaa gtaagatggg ccgtcaaatc aaacgataca agggcgatga gctcaaagaa
     1261 gcgctcgcct tggttatgca gcagcctcaa gcacaaaaac gactaagaca gcgcaaagcc
     1321 atggtagaac cagtcttcag tcacattaga attcgtcaaa actttaatcg ctttaagcgg
     1381 agagggcttc atggcgtaaa agtcgaattt acactgcaaa tcatggctta taacatcagt
     1441 cgagctatcg ctcgatattt ttctttcctt attcgattga taattaacca tattggtaat
     1501 tttgtattct aatgccataa gtcatttcaa atacaacaat aaatagacag
//
LOCUS       DATNSN010000304         1539 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.307, whole genome shotgun sequence.
ACCESSION   DATNSN010000304 DATNSN010000000
VERSION     DATNSN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.307"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            115..>1539
                     /locus_tag="VAH76_003140"
     rRNA            115..>1539
                     /locus_tag="VAH76_003140"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          446 a          283 c          495 g          315 t
ORIGIN      
        1 gagagcattt tattctggta tagccggagt tcattaacaa gatggtaaag aagaagaggt
       61 aacacaagcg attggtatat gcatcatgtg attttgaggt gattgagatt atatggtcaa
      121 gaagagaagc gcaaacggtg gatgccttgg cagtaagagg cgaagaagga cgtggaatcc
      181 tgcgaaaagc tatggggagc tggaaacgag cgttgagcca tagatgtccg aatgggggaa
      241 cccggctgca gcgatgcggt catttgcatt tgaatacata ggatgcaaag gcgaactcgg
      301 ggaactgaaa catctaagta cccgaaggaa aagaaatcaa gagagattct ccaagtagcg
      361 gcgagcgaac ggggaggagc ctggcgtgat ttattattga actgagtaga acaatttggg
      421 aaagttggcc gaagagggtg aaagccccgt atacgaaggt ttgatgagga actaggcacg
      481 cgaacaagta ggccgggaca cgtgaaatcc tggttgaaga tgggtggacc atcatccaag
      541 gctaaatact acttactgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagaac
      601 cccggagagg ggagtgaaat agaatctgaa accgtttgcg tacaagcagt gggagcatgg
      661 tttaggctgt gtgactgcgt accttttgta taatgggtca gcgagttact ttcagtggcg
      721 aggttaactg aataagggag ccgtagagaa atcgagtctg aatagggcga tagtcgctgg
      781 gagtagaccc gaaaccgggc gatctagcca tgtgcaggat gaaggttggg taacaccaac
      841 tggaggtccg aaccgggtaa tgttgaaaaa ttatcggatg acgtgtggct aggagtgaaa
      901 ggctaatcaa gcccggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg
      961 aatgattact gggggtagag cactgtttcg gctagggggc tgtcatggct taccaaaccg
     1021 atgcaaactc cgaataccgg ctaattgaat cacgggagac acacggcggg tgctaacgtc
     1081 cgtcgtggag agggaaacaa cccagaccgc cagctaaggt ccccaagtac tagttaagtg
     1141 ggaaacgatg tgggaaggca tagacagcca ggaggttggc ttagaagcag ccacccttta
     1201 aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa cggggctaaa
     1261 actagtcacc gaagctgcgg atgtgcgcga aggcgcacgt ggtaggggag cgttctgtag
     1321 gctgatgaag gtgcattgag aagtgtgctg gaggtatcag aagtgcgaat gctgacatga
     1381 gtaacgataa tgtgggtgaa aagcccacac gccggaagtc ccaggtttcc tgcacgacgt
     1441 taatcggagc agggtgagtc ggcccctaag gcgaggctga agagcgtagt cgatgggaac
     1501 caggttaata ttcctggact ttttataagt ggtgaagtg
//
LOCUS       DATNSN010000305         1515 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.308, whole genome shotgun sequence.
ACCESSION   DATNSN010000305 DATNSN010000000
VERSION     DATNSN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.308"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(140..367)
                     /locus_tag="VAH76_003141"
     CDS             complement(140..367)
                     /locus_tag="VAH76_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387712.1"
                     /translation="MKKFHVSLLTAMVVSWMNIAYADEQSCNKSYDYCMSSCENQYNQ
                     CTKNNDESYCKEQYQPCIQGCSNTRDSCLSQ"
     gene            complement(530..>1515)
                     /gene="aph(9)-Ia"
                     /locus_tag="VAH76_003142"
     CDS             complement(530..>1515)
                     /gene="aph(9)-Ia"
                     /locus_tag="VAH76_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HEO1387713.1"
                     /translation="PDQQIIELLKVYYGIDIHTVQLIVGGADMNAFGYKADSKSNSYF
                     VKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQMDHVKIIVYPFIDAPNG
                     FTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREMVRSFYSQIGFDKC
                     DDQITTDFKSFFNQNIDLIHQLVNSSEELSKKIQPDFEKYVLCHSDIHAGNVLVVDEE
                     SIYIIDWDEPMFAPKERDLMFIGGGIGNVWNKPHEIDYFYEGYGKTSIDKTILSYYRH
                     ERIVEDIAVYGHELLSRDQNAQSRLESFKHFKSMFDPNDVIEIAFASDRCH"
BASE COUNT          486 a          264 c          246 g          519 t
ORIGIN      
        1 agacgccagt ttatataatt gtaaattcgt attaaaaatt attttgtttt ccggagacgc
       61 aaaaatagat aaattaaaaa caattattag gaagaaaaaa acacttacct gtgatatctt
      121 catttttgtc ctttcggtgt tactgagata agcagctatc tctagtatta ctgcagcctt
      181 gaatacaggg ttgatattgc tccttacagt aagattcatc attattttta gtacattgat
      241 tatattggtt ttcgcatgag ctcatacaat aatcgtaact tttattacaa ctttgttcat
      301 ctgcataggc tatattcatc caactgacaa ccatggcggt gagcagtgac acatgaaatt
      361 ttttcattga ctcgtctcct tcatttgagc tgaaaaaatg tatattttga cttaacccaa
      421 tagtcaaaaa ttaaatccca ttccttgttt agataagtgt agcaggaaat atcaaatctg
      481 ttttccattc ttgctgagtg gattggctag ttctaattgt aatacggatt tagtgacaac
      541 gatctgatgc aaaagctatt tcaataacat catttggatc aaacattgac ttaaaatgtt
      601 taaaactttc aagtctggac tgagcatttt gatcacgtga aagcaattca tgtccataca
      661 cggctatatc ttcaacaatt cgctcatgtc tgtaataaga caaaatggtt ttatcgatgc
      721 ttgttttacc ataaccttca taaaaataat cgatttcatg aggtttattc catacattac
      781 caatgccacc gccaatgaac atcaaatcac gttcttttgg tgcgaacatg ggctcatccc
      841 aatcaataat gtaaatagac tcctcatcaa cgaccaacac attgcctgca tgtatgtcag
      901 aatgacaaag tacatatttt tcgaaatcag gttgaatttt tttagacagc tcctctgaag
      961 aattaactaa ttgatggatt aagtcgatat tttgattaaa aaaagatttg aagtcagtgg
     1021 taatttgatc atcacactta tcaaatccaa tttgactgta aaaagacctt accatttcac
     1081 gccatttagg cgagtaagtt tcttttctta atcgctgttg aatcgaggtg ggaacggagg
     1141 ttccatggat ttgtcttaat attttcccaa gctgtttcca ctgtttttct gttaaatttt
     1201 tggtgaaacc attgggcgca tcaataaatg gatacacaat tattttaaca tgatccattt
     1261 gctgaaataa gtttgcttcg agtgtgttga taggaaaaac aatttccttt actccagagt
     1321 catgcaagag acggatgatt gataaattaa tttcatcatg atggccgtat tttagcttta
     1381 cgaaatagga attggatttt gaatctgctt tatatccaaa ggcattcata tcagcaccac
     1441 ccacgataag ttgtacagta tgaatatcaa ttccataata aactttcaaa agttcaatga
     1501 tttgttgatc cggaa
//
LOCUS       DATNSN010000306         1494 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.309, whole genome shotgun sequence.
ACCESSION   DATNSN010000306 DATNSN010000000
VERSION     DATNSN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.309"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1425
                     /locus_tag="VAH76_003143"
     rRNA            <1..1425
                     /locus_tag="VAH76_003143"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          381 a          317 c          448 g          348 t
ORIGIN      
        1 acttgcatgt aggggggaag acttggcaaa tccggttttc cataactctg aggtgcgaag
       61 tggttctgac cttttggtac agagaagtca ttgatgcccg gcttccagga aaagctgcta
      121 gccataactt atagagaacc gtaccgcaaa ccgacacagg tggacaggta gagaatacta
      181 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtaccgtaa cttcgggaga
      241 aggtacgccc tttctggtga tgggatttac tttcagagct ggagagggcc gcagagacca
      301 ggtggctgcg actgtttatt aaaaacacag cactctgcaa attcgtaaga agacgtatag
      361 ggtgtgacgc ctgcccggtg ccggaaggtt aattgatggg gttatcttcg gagaagctct
      421 tgatcgaagc cccggtaaac ggcggccgta actataacgg tcctaaggta gcgaaattcc
      481 ttgtcgggta agttccgacc tgcacgaatg gcgtaacgat ggccacactg tctccacccg
      541 agactcagtg aaattgaaat cgctgtgaag atgcagtgta cccgcggcta gacggaaaga
      601 ccccgtgaac ctttactata gttttgcact ggactttgat gatgactgtg taggataggt
      661 gggaggctgt gaagtgagga cgctagttct catggagccg cccttgaaat accaccctgt
      721 tgttattgag gttctaactt ggtccagtaa tcctggatga ggacagtgta tgatgggtag
      781 tttgactggg gcggtctcct cccaaagagt aacggaggag cacaaaggta ccctcggtac
      841 ggtcggacat cgtaccaaga gtgtaaaggc aaaagggtgc ttgactgcga gagtgacggc
      901 tcgagcagga acgaaagttg gtcttagtga tccggtggtt ctgaatggaa gggccatcgc
      961 tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag ttcatatcga
     1021 cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag caggtcccaa
     1081 gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg tcgtgagaca
     1141 gttcggtccc tatctgccgt gggcgtagga aaattgagag gagctgctcc tagtacgaga
     1201 ggaccggagt ggacgaacct ctggtgcacc ggttgtcacg ccagtggcat ggccgggtag
     1261 ctaagttcgg acgggataac cgctgaaagc atctaagcgg gaagcctccc tcaagatgag
     1321 ttttcccatg aagcccgttg aagactacga cgttgatagg caaggtgtgg aagcgcagta
     1381 atgcgtgaag ctaacttgta ctaattggct gattgtcttg accatataat ctgagtgact
     1441 tcagaatgtg atattgattt gtatacgtga aacgtatcgt gtaaactctg actc
//
LOCUS       DATNSN010000307         1465 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.310, whole genome shotgun sequence.
ACCESSION   DATNSN010000307 DATNSN010000000
VERSION     DATNSN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.310"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..811)
                     /locus_tag="VAH76_003144"
     CDS             complement(<1..811)
                     /locus_tag="VAH76_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387714.1"
                     /translation="MKIFKGLKKNTGFRKQSNTQKISNSSIQLSITSLSGDASQPRFD
                     FFPINNRDVLLKSPSFNHVGSEFQPMVSKRDVNSISITLPIKHNVHRNHDGNLPGDSI
                     SVVDSSNQQDHISYLQLCFTHEEGKWYLFLKCLTTLDVFNYDFAPLLKILHKPDIRTY
                     PMSLGCPGVIDRTELVNMDRTIYVRQFIIHGPDDEAKILQDIHDFNMNCLRRFEELTY
                     GRDNSAYRNSQALGRILHKIITAYQEVTVNEYDIALNRLKIVFQDQVFQNDP"
     gene            1263..1339
                     /locus_tag="VAH76_003145"
     tRNA            1263..1339
                     /locus_tag="VAH76_003145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Arg,seq:cct)
BASE COUNT          469 a          279 c          233 g          484 t
ORIGIN      
        1 gtggatcatt ttgaaatact tgatcttgaa acacaatctt aagccggttc agtgctatat
       61 catattcatt tacagttact tcttgataag cagttataat tttatgcaaa attctcccca
      121 gagcttgaga gtttctataa gcactattat ctctaccgta ggttaactct tcaaaccgcc
      181 tcaaacagtt catattgaaa tcatgaatat cttgcaagat tttagcctca tcatcaggcc
      241 catgtataat aaactgcctg acataaattg ttctatccat gttaacgagt tctgtcctat
      301 caataacccc agggcaaccc aaactcatgg gataagttct tatgtctggt ttatgtagta
      361 ttttcaataa tggtgcaaaa tcataattaa aaacatccag agttgttaaa cactttaaaa
      421 ataaatacca ctttccttct tcatgagtaa agcaaagctg taaatatgaa atatgatcct
      481 gttgattaga tgagtccacg actgatattg aatcacctgg caaattacca tcatgatttc
      541 gatgaacatt gtgtttaatt ggtaatgtaa tcgagattga gtttacatcc ctcttggata
      601 ccatgggttg aaattctgaa ccgacatgat tgaaagaggg gctttttaat aaaacatctc
      661 tattgtttat tggaaaaaaa tcaaatcggg gttggcttgc atcgccagat aaagaagtaa
      721 tggataattg aatcgatgaa ttagatattt tttgagtatt tgactgcttc ctaaaaccag
      781 tgtttttctt taatccttta aaaattttca tatttaacat ctcctttaat tactcaaatt
      841 ttgcaatcca ttatcaacta gcgcaaggta tcttaattat taatacgcgg tcaatagata
      901 ttgtttcatg tcgtactgtt atcaggttat atccatcttg gtagccaatc acaaaacctt
      961 caccaggttt aataaaaatt gacttaccga tttccagaat tgagaaatga aagaataatt
     1021 aagcagggct tacgaaaacc cccaatctct atgttaaaaa atgttttaat tcggtcattt
     1081 agtttatcta aactccctca ttaaaaacat tttttgcctt gaccttgggg tttttcataa
     1141 gtcctgctaa gaacacatcc caatccaacc agccctatca ctattttcat ataagaaatt
     1201 tgctttcccg ttgtcatttt gcgcctataa gggtagactt gcgtttgata aataaagtca
     1261 aagtcccggt ggcacagatg gatagcgcag ccccctccta aggggcaggt cgcaggttcg
     1321 aatcctgccc gggacgccaa tgaaaccagg gcctattcca aagaaaatta ccaagattat
     1381 ataattcaat aaatcggggc tttaaaaata ttgtaaccta aagcattctt gctttaagtc
     1441 atattctaca gactattgac ttttt
//
LOCUS       DATNSN010000308         1428 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.311, whole genome shotgun sequence.
ACCESSION   DATNSN010000308 DATNSN010000000
VERSION     DATNSN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.311"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..858)
                     /locus_tag="VAH76_003146"
     CDS             complement(<1..858)
                     /locus_tag="VAH76_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387715.1"
                     /translation="MFFELDVFFVLIFLFSLLSSNAYSEKVNSISLFTYPVEAIYIIN
                     NPRTGEMVMGSDYHGACNLLGEQYFWGVVPSKNEKPACYSSTSYNMVIGADTWYCSDG
                     TNAYFNVPHNYCLNFIVCPDASWTLSENKEYCIRQDLPCIPDPENISEEQALAALAYG
                     ESHWSNDYKEMAGIASAARRRMKAMGFKSINELILKDKNFAYATAPKIRNERYYNIIC
                     GIDSPGVELAYKASRNALNDGTDYSNGACFWDGVDLKVNGENAYRYKARFKLANKNHN
                     VLSVIEPPPL"
     gene            complement(1016..1384)
                     /locus_tag="VAH76_003147"
     CDS             complement(1016..1384)
                     /locus_tag="VAH76_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase"
                     /protein_id="HEO1387716.1"
                     /translation="MSDFVYVLMERRSRNKTSEFVFQAESKTGYIYEPKKAVNRVVEL
                     SGVPFTLPDLRRTFATIADSLDLPAYALKRLLNHKMNNDVTAGYIMKDAERLRKPMQQ
                     VANFLLKHIEGDNSLNIMVI"
BASE COUNT          455 a          303 c          226 g          444 t
ORIGIN      
        1 taatggtggt ggttcaatta cagaaagaac attatgattt ttattagcaa gcttaaatct
       61 tgctttatat ctataagcat tttctccatt taccttcaaa tcaactccat cccaaaaaca
      121 tgccccattt gaataatcag ttccatcgtt tagagcattc ctggaagctt tatacgctaa
      181 ttctacccct ggggaatcga ttccacatat gatattgtag taacgctcat tccttatctt
      241 gggagcagta gcataagcaa aatttttatc ttttaatatc agttcattaa tactcttaaa
      301 tcccattgct ttcattcttc ttcgggcagc acttgcaata cctgccattt ctttataatc
      361 gttagaccag tgcgactcgc cataggctaa agcagccaac gcttgctctt ctgaaatatt
      421 ttctggatcg gggatacaag gcaaatcctg acgtatgcag tattccttgt tttcagacag
      481 tgtccaactt gcatcaggac aaacaataaa attcagacaa taattatgcg gaacattaaa
      541 ataagcattt gttccatcag agcaatacca tgtatctgct ccgataacca tattgtaaga
      601 agtgcttgaa taacacgccg gtttttcatt tttcgagggt actactcccc aaaaatactg
      661 ctcgcctaat aaattgcaag ctccatgata atctgagccc atgaccatct cgccagttct
      721 tggattattg atgatgtata ttgcttcaac aggataagtg aatagagaaa tactattcac
      781 tttttcgcta tatgcattac tagataacaa gctgaacaaa aatatcagta caaaaaaaac
      841 atccaattca aaaaacatcc atcatatgaa accgcaatca actttatttc tctagatata
      901 tagtgaaata aaattcattt ttaaaagcca gaaaaaacaa gagcctccga agaggctctg
      961 cgccccagga gagaagtcct aggggatgta gtaaattaat tatactaaat attcgtcaaa
     1021 tcaccatgat atttaatgaa ttatcccctt caatatgctt tagcaagaag ttagcaacct
     1081 gctgcatcgg cttacgcaac cgctcagcat ctttcataat atatcctgct gtaacgtcat
     1141 tgttcatttt atgattcagc aagcgtttta aagcataagc aggcaaatca aggctatccg
     1201 cgatagtcgc aaaagttcta cgaagatcag gcaaggtaaa tggtacgcca gacaactcca
     1261 ctaccctatt cactgctttt ttaggttcat agatataacc tgttttactt tcagcctgga
     1321 atacaaattc actggtttta tttcggcttc tgcgttccat taacacatag acaaaatcag
     1381 acataggcaa gatgtatttc ttgatttttg ggtttattcg cgcctaat
//
LOCUS       DATNSN010000309         1427 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.312, whole genome shotgun sequence.
ACCESSION   DATNSN010000309 DATNSN010000000
VERSION     DATNSN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.312"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(67..678)
                     /locus_tag="VAH76_003148"
     CDS             complement(67..678)
                     /locus_tag="VAH76_003148"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO1387717.1"
                     /translation="MVYSVGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKNT
                     PCYQDIISHFGSSVVLNNGELDRKRIRDIIFSNSNERLWLESLLHPVIRKKIEEQLIV
                     CTSPYCLIEIPLLFNKHHYPYLQKVLLVIAPLESQLDRIVKRDHCTKEQALAILATQP
                     NLEQRLEAADDVLINESGLSELKAKVNKLHQKYLREAKIKQKQ"
     gene            complement(650..1123)
                     /locus_tag="VAH76_003149"
     CDS             complement(650..1123)
                     /locus_tag="VAH76_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387718.1"
                     /translation="MRNYIFYGLLLLIGLSHQGFTAPMMKKGSYWKCVTYDKANKAWT
                     AQSSYRKVAINVAFAACKKESQLPATCKTSISSCEGFIDGVSTRPMWRCTAMDITAQP
                     WESNFYSNRDDAALAAQAYCKENSTLPATCYINMVTCANKNEGAHSDGLFSGTNR"
     gene            complement(1120..>1427)
                     /locus_tag="VAH76_003150"
     CDS             complement(1120..>1427)
                     /locus_tag="VAH76_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1387719.1"
                     /translation="VDDYGTGHASISYLQHFPFNYLKIDKLFVQAIGSNDIIESLNDA
                     IIQMAKGLNLVTIAEGVETKDQANYLANNGVRLQQGWYYSKGLSIAELTALLKGEKI"
BASE COUNT          423 a          262 c          254 g          488 t
ORIGIN      
        1 cataaaagtt gttgaaaata aaaaaatctt taaaatccat atattaactg gtcttttgct
       61 agaaatttat tgcttctgtt tgatttttgc ttccctaaga tatttttgat gaagcttatt
      121 caccttggct tttaattcac tcaatccaga ttcattgatc agcacatcat ccgctgcttc
      181 caagcgttgc tctaaattag gttgtgtggc taaaatggct aatgcttgtt cttttgtgca
      241 atgatctctt ttaacaattc tgtctagctg agattccagg ggagcaataa ccaatagaac
      301 tttttgaaga taggggtagt gatgtttatt aaacaacaaa ggaatttcaa taaggcaata
      361 gggcgaagtg cagacgatga gttgctcttc aattttttta cggatgactg gatgtaatag
      421 actttcaagc cataagcgct catttgaatt ggaaaatata atatctctta tacgcttccg
      481 atcaagctca ccattattaa gaaccactga agaaccaaaa tgtgaaataa tatcctggta
      541 gcagggggta tttttagaag ttaattcttt ggcaatttta tctgcataaa taacatttat
      601 acccagttcg gaaaaaaatt ctgcgactgt acttttgcca ctggcaatat tacctgttag
      661 tcccactgaa taaaccatct gagtgggctc cttcattttt gtttgcacaa gttaccatat
      721 taatataaca agtagcaggt aatgtactgt tttctttaca ataggcctgg gcggctaagg
      781 cggcatcatc tcgatttgag tagaaattac tttcccaagg ttgggcagtt atgtccatgg
      841 ctgtacatcg ccacattggt ctagtgctta ctccatctat aaatccttca caacttgata
      901 tggatgtctt acatgttgcg ggaagttgac tttctttttt gcaggcagca aatgcaacat
      961 tgatagccac ttttcggtat gagctttgcg ccgtccatgc tttgttggct ttatcataag
     1021 ttacgcattt ccaataactt ccttttttca tcattggggc agtaaagcct tgatgggata
     1081 aaccaattaa tagtagtaaa ccataaaaaa tataattcct catatttttt ctcctttaag
     1141 caaggctgtt aattcagcaa tggatagccc tttggaataa taccagcctt gttgtaaacg
     1201 aacaccatta tttgccaggt aattagcttg gtcttttgtt tccacgcctt ctgctatagt
     1261 aactaagttt aatccttttg ccatctggat aatggcgtca tttaatgatt cgataatatc
     1321 attagaacca attgcttgaa caaataattt atcaattttt aaataattga aaggaaagtg
     1381 ttgcaaataa ctaatgctgg catggccagt cccataatca tcaacag
//
LOCUS       DATNSN010000310         1421 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.313, whole genome shotgun sequence.
ACCESSION   DATNSN010000310 DATNSN010000000
VERSION     DATNSN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.313"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1421
                     /locus_tag="VAH76_003151"
     CDS             <1..>1421
                     /locus_tag="VAH76_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HEO1387720.1"
                     /translation="NTPFTIETTSLLGENTDLFGLYETEGVALVKAESEGLRRVLYLP
                     DRPDNLATYKTTIIANQEDYPVLLSNGVLIEKKELPLGLHSATWLDDVPKPSYLFALV
                     AGNLQRSVTYYQTKSGRELPIEFYVPPSATAKCDFAKEVLKEAMAWDERTFNLECALR
                     QHMVAGVDKYASGASEPTGLNLFNTENLFATPETKTDLGILRVLEVVAHEFFHYWSGD
                     RVTIRDWFNLPLKEGLTTFRAAMFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSL
                     YTAAAYEKSADIFRMMMLFIGKKTFIQGVAKFFKDNDGSAVTLEDFIDSLSNSSGKDL
                     RSFLSWFTESGIPELIVTDEFNPDAKQYLLKIKIANGKNRPVPFLMGLLDSSGKEIVA
                     DTLLMVDQEEIEFQFENIQTRPIPSLLRSFSAPVHMKYEYSYQDLLLLMQFDTNLYNR
                     CEAAKRLISVLINDFCIGKKIEL"
BASE COUNT          438 a          237 c          297 g          449 t
ORIGIN      
        1 aaatactccc tttactatag aaacgacctc tctgctcggt gaaaataccg atttgtttgg
       61 attatatgaa acagagggag ttgccttggt taaggcagag tctgaaggat tgcgccgagt
      121 attatatttg ccggacagac ctgataatct cgcgacttat aaaactacta tcatcgcgaa
      181 tcaggaagac tatcctgttt tactttcaaa tggtgtgcta attgagaaaa aggaacttcc
      241 tttgggcctg cattccgcaa cctggctgga tgatgtccca aaaccaagtt atttatttgc
      301 tctggtagca ggtaatttac agcgttcagt cacttattat caaacaaaat ctggccgtga
      361 attacccatc gaattctatg ttccaccatc agctacagca aaatgtgatt ttgcaaagga
      421 agtattgaaa gaggcaatgg catgggatga acgtactttt aatttagaat gtgccttgag
      481 acaacatatg gtagccggag ttgataaata cgcgtcaggt gcttccgagc caactggctt
      541 aaatttattt aatacagaaa atttatttgc aactcctgaa actaaaaccg atttaggtat
      601 tttgcgcgtg ttggaagtgg ttgcccatga gttttttcat tattggtcag gagatcgggt
      661 cacaataaga gattggttca atttgccatt aaaggaaggt cttactacgt ttagagctgc
      721 tatgttcaga gaggagctgt ttggtacgga tttgataaga ttacttgatg gcaagaattt
      781 ggatgaaaga gcaccacgac aaagtgccta tacagcagtt agaagcctat atactgctgc
      841 agcctatgaa aaaagtgctg atatttttcg tatgatgatg ttgtttatag gtaaaaaaac
      901 atttattcaa ggggtagcca agttttttaa ggataatgat ggaagcgcag ttactctgga
      961 ggattttatt gattcactta gcaattcatc tggtaaagat cttcgttcct ttttatcctg
     1021 gtttactgaa tctggaattc ctgaattgat agtaacggat gaatttaatc cagatgcaaa
     1081 gcaatatctt ttaaaaatta aaatagccaa cgggaaaaac cgccccgttc ctttcctgat
     1141 ggggttgctt gatagctcag gaaaagaaat tgtagctgat acattattaa tggttgatca
     1201 agaggaaatc gagtttcaat ttgaaaatat ccagacccgc cctattccat cgttattacg
     1261 tagtttttct gcaccggtac acatgaaata tgaatattct tatcaggatt tattattgtt
     1321 gatgcaattc gacactaatt tgtataatcg ttgtgaggca gcaaaacgac ttatttcagt
     1381 tttaattaac gatttttgta taggaaaaaa aatagaatta t
//
LOCUS       DATNSN010000311         1347 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.314, whole genome shotgun sequence.
ACCESSION   DATNSN010000311 DATNSN010000000
VERSION     DATNSN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.314"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..183
                     /locus_tag="VAH76_003152"
     CDS             <1..183
                     /locus_tag="VAH76_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO1387721.1"
                     /translation="NLNNSCGSLTSNEKQKILSWLEYIGEKDPLIIIEVLEFCRNNVD
                     SRQYFLQRAKEVPEIK"
     gene            243..617
                     /locus_tag="VAH76_003153"
     CDS             243..617
                     /locus_tag="VAH76_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387722.1"
                     /translation="MKFKPGESGNPSGRPKGSRNIQTQLIKLLEPHAEQLINKMVEKA
                     LEGDPHALRLCIERLLPKAKHNPIESDLLTLEEDNYMETIESILQEMLLGNIASDEGK
                     KIIDLIEEHQNRKRFDSLIHIP"
     gene            complement(631..>1347)
                     /locus_tag="VAH76_003154"
     CDS             complement(631..>1347)
                     /locus_tag="VAH76_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387723.1"
                     /translation="AGKPLHSKEITALALKLGRLNSAGKTPDLTMNAELNVDINTKKA
                     ASCFIKVAPSVFAINNDKYLSSDLKLMADKEYKISPNISTQQKGHIAEARIAELITLY
                     GDTTLSCYRPVSDDEGIDLIVKEKGSLKTIYLQIKSRFESNPSRSFTSTIRSCNLHDH
                     HAMAVIFCLFDTDAGDLCNQLWFVPACSLIKNAHKLQNGDFFGFVAGKRKKESNKWDE
                     FLIDKRDLANQIIASIKRIS"
BASE COUNT          466 a          211 c          249 g          421 t
ORIGIN      
        1 aacttgaata attcatgcgg atcgttaacc agtaatgaaa aacaaaaaat actttcttgg
       61 ttagaatata ttggtgaaaa agacccttta atcattattg aagtgttgga attttgcaga
      121 aataatgtag attcacgaca atatttttta caaagagcga aagaagtacc tgaaataaaa
      181 taagccagaa aatgaattaa acgccgtcac ctgtaaataa cagataatta acagagatag
      241 ttatgaaatt taagcccgga gaaagcggta atccatcagg tcgaccgaag ggatctcgga
      301 atattcaaac ccagttgatt aaattgcttg agccgcatgc agagcagttg ataaataaaa
      361 tggttgaaaa ggcgctagag ggagatcctc atgcgctacg gctttgtatt gagaggttat
      421 taccaaaagc gaaacataac cctatagaga gtgatttact aactctagaa gaagataatt
      481 atatggagac aattgagtct attttgcagg aaatgctgct aggaaatatt gcttctgatg
      541 aaggcaaaaa aattatcgat cttattgaag agcatcaaaa taggaagagg tttgactcat
      601 tgatacatat tccataatgc tagcgataaa ttaagaaatg cgttttatcg atgcaatgat
      661 ttgattggcc aggtctcttt tatctattaa aaattcatcc catttatttg attctttttt
      721 acgttttcca gctacaaagc caaaaaagtc tccgttttga agtttatgag cattctttat
      781 aagtgaacag gcaggaacaa accataattg attacaaaga tctccggcgt cagtgtcaaa
      841 taaacaaaaa attacagcca tggcatgatg atcatgtaga ttgcaagatc taattgtaga
      901 ggtaaatgat ctagaaggat tgctttcaaa tctgcttttt atttggagat atatagtttt
      961 taaacttcct ttttctttaa caataaggtc aataccttca tcatcagaaa caggccgata
     1021 acaagataag gttgtatctc cgtaaagtgt aataagttcc gcaattctgg cttctgcaat
     1081 atgccctttt tgttgagtgg aaatattagg tgaaattttg tattccttat ctgccataag
     1141 cttaaggtcg cttgatagat atttatcatt attaattgca aaaacagaag gtgctacttt
     1201 aatgaaacaa gaagcagctt ttttagtatt aatatcaaca tttaattcag cattcatcgt
     1261 taaatcagga gttttccctg ctgaattcaa tctcccaagc tttaaggcaa gggctgttat
     1321 ttctttacta tgcagtggct tacctgc
//
LOCUS       DATNSN010000312         1295 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.315, whole genome shotgun sequence.
ACCESSION   DATNSN010000312 DATNSN010000000
VERSION     DATNSN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.315"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            16..1179
                     /locus_tag="VAH76_003155"
     CDS             16..1179
                     /locus_tag="VAH76_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO1387724.1"
                     /translation="MQNGQVIPFYHSTNELAHIQNILQSQTPLVSLKEINKFRQLIAR
                     ASAGEYFLIQSGDCADNFYECDKDTTHAKLDFIRHLATFFHQKTGCSVIQVGRIAGQY
                     AKPRSNQYEICQNKRIYSYFGDMINQSQETQRRPDPWRILLSYNCSASIYRKINQWNR
                     GINPEQKIYTSQEAFLFNYEQSLIRWDERTGLYYNLSTHFPWLGKRIVSSLQHINHLK
                     KIANPIAVKIGPDTPIKSLVKTINELDPSYIPGRITLIPELGAQNVYHILPQLIDAVK
                     RTDRTVLWSCDPMHGNTETLSSGIKIRKLSNIVDEIKNSFFIHRKMGSQLNAIHLEAT
                     HQNVTECMDYNSSRCESISDLKKNYKSLLDPRLNYAQTMHVIQCVAKHYVNSY"
BASE COUNT          467 a          231 c          227 g          370 t
ORIGIN      
        1 gtccaacaac gtacaatcca gaatggtcaa gttattccat tctatcattc cactaatgaa
       61 ctggcacata tccaaaatat tctacagtct caaaccccat tggtatcttt gaaagagatt
      121 aataaattcc gtcaattgat agcaagagca tcggcaggag agtatttttt aatacaatcg
      181 ggtgactgtg ccgacaattt ttatgagtgt gacaaagaca caacccatgc aaaattagat
      241 tttattcgtc acttagccac attttttcat cagaaaactg gctgtagcgt gatccaggtt
      301 ggtcggattg cagggcaata tgcgaagcct agaagcaatc agtatgaaat ttgccagaat
      361 aaaagaatct attcatattt tggtgacatg attaatcagt ctcaagaaac tcaacgacgt
      421 cccgaccctt ggaggatatt gttgagttat aattgttcag catctattta tcgcaaaatt
      481 aatcaatgga atcgaggaat aaacccagaa caaaaaatat atacaagtca agaagcattt
      541 ttatttaatt acgaacaatc attaataagg tgggatgaac gaacaggatt atattacaac
      601 ctttcaacac atttcccatg gcttggcaaa aggatagtaa gcagcttaca gcatattaac
      661 catttgaaaa aaattgcaaa tccgattgcc gtgaaaatag gcccggatac ccctatcaag
      721 agtcttgtaa agaccatcaa tgaattagat ccaagttata ttcctggacg tatcaccctt
      781 attcctgaat taggagcgca aaatgtctat cacattttac cgcagttaat tgatgcagtg
      841 aaaagaacag atcgcactgt tttatggagt tgtgatccca tgcatggaaa tactgaaact
      901 ctaagttcgg gaataaagat tagaaaacta agtaacattg ttgacgaaat caaaaattca
      961 ttttttattc atagaaagat gggtagccaa cttaatgcta tccatcttga agcaacacat
     1021 cagaatgtca cagagtgcat ggattataat tcatctcgtt gtgaatcaat aagcgacctt
     1081 aagaaaaact ataaaagtct attagaccct cgattaaatt atgcacaaac gatgcatgtc
     1141 attcagtgtg ttgcaaaaca ttacgtaaat tcttattaaa aggcctggac atgggcgaac
     1201 tgaaaaaaat gagcttattt agctttaacc aacatatcca gaaaaataat tttaaacact
     1261 gttaattgaa ttaaggagta attatgaaca gtttg
//
LOCUS       DATNSN010000313         1288 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.317, whole genome shotgun sequence.
ACCESSION   DATNSN010000313 DATNSN010000000
VERSION     DATNSN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.317"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            57..1130
                     /locus_tag="VAH76_003156"
     CDS             57..1130
                     /locus_tag="VAH76_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387725.1"
                     /translation="MSANEKALMQELKEAIKIKTLTQSHLTKEEQHLIVVYLAEIKEF
                     YKLNSIFDFFGVENGGAQHGRLFFHDSENNSSRELTELFRLNKALLNDLNEKIEQVAI
                     EYSKENYDYIWSQIIFSYLKLLEHNQHWQNFWEKESQVLDEAKLNKAKHLVCSAICFK
                     LFSVLSANSIEMMSHPAVKVFIKLSNSINFEDVRKEMRNRLRSEILEDIKIATMTKKN
                     SKLTYKSLYAKANQDAHFCALSSEEKALGIIAGVADACPGNDIHFKLLMECIVKAKKQ
                     FPVDDFNTKIQTILEELQAKSKLPQKYEKKFQEICETQDNKRILKKTLSLPQLSSVGS
                     TFYHSKKREGFSDENTNSYPHRP"
BASE COUNT          460 a          207 c          227 g          394 t
ORIGIN      
        1 taaattacaa gtgattggat atttaatttt tctttttaag aacataggaa ccaaacatga
       61 gtgcgaatga aaaagcttta atgcaagagt tgaaagaggc gataaaaatc aaaactctta
      121 cccagtcaca tttaacaaaa gaagaacaac atctaatcgt cgtatatctg gcagaaataa
      181 aagagttcta taaattaaat tctatctttg atttttttgg ggtagaaaat ggtggtgcgc
      241 aacatgggcg attattcttt catgactctg aaaacaattc atccagggag ttgactgaac
      301 tcttcagatt aaataaggca ttattaaatg atctaaatga gaaaattgag caggttgcta
      361 tcgaatatag caaagaaaat tatgattata tctggagcca gatcattttc tcctatttaa
      421 aattgctgga acataatcaa cattggcaga atttttggga aaaagagtca caagttctgg
      481 atgaagccaa attaaataaa gcgaaacatc tcgtttgcag tgcgatatgc tttaaactat
      541 tttctgtgct ctcggccaat tcgatagaaa tgatgagcca tcccgctgtt aaagtcttta
      601 tcaaattgtc aaatagcatc aattttgaag atgttcgaaa agaaatgcgc aatcgtttgc
      661 gtagtgaaat tttagaggat attaaaattg caactatgac aaaaaagaat tccaaactga
      721 catacaagtc attatatgca aaagcaaacc aggatgctca tttctgtgct ttgagctcgg
      781 aagagaaggc tttaggtata attgcaggag ttgcagatgc ctgtccggga aatgatatcc
      841 attttaaatt attaatggaa tgtattgtga aagcaaaaaa gcaatttcct gtcgatgatt
      901 ttaatacaaa aatccagacg atacttgagg aattgcaggc taaaagcaag ctcccgcaaa
      961 agtatgaaaa aaaatttcag gaaatttgtg aaactcaaga caacaaaaga atattgaaaa
     1021 aaactttgtc attaccacag ctttcttcag tgggttcaac tttttatcat tctaaaaaaa
     1081 gagagggttt ttctgatgaa aatacaaact cttatcctca tagaccatag gttgcgctag
     1141 caatcattct tcatttccta cgtaccgcga catgctcgcg gtcagctaca tatttcttca
     1201 taaagatcac attattctgg atttagtcct tcaatcaaat ttaatttcta ctgtctgtac
     1261 tagtttctga agttattttc acattcat
//
LOCUS       DATNSN010000314         1285 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.318, whole genome shotgun sequence.
ACCESSION   DATNSN010000314 DATNSN010000000
VERSION     DATNSN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.318"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            185..595
                     /locus_tag="VAH76_003157"
     CDS             185..595
                     /locus_tag="VAH76_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEO1387726.1"
                     /translation="MSKKSIFLATLALIFIFGQPVFANSSDATQTKPCHCHEHKKILS
                     QLNLTQEQKTKIKTIKIQAQKSIKENYRQLKAIKEQINALIANEKLDEAKLDSLINQR
                     NKIAGTMLKNRVMMKNQIYNQLTNEQKSQYKKNM"
     gene            1103..>1285
                     /locus_tag="VAH76_003158"
     CDS             1103..>1285
                     /locus_tag="VAH76_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387727.1"
                     /translation="MRGHMIFLSIPKGMEFKQITEKDNTNDYFVDPNGKLPRINIQAL
                     VKDALQYNKGRKKEISL"
BASE COUNT          499 a          232 c          194 g          360 t
ORIGIN      
        1 tacatgacaa aaatatgtca tgcataaaag actatgtatg accacagtaa ttagacaaag
       61 cctattagga aataagtcta tattaatttg tacattttga tttatactgc tatcagcatg
      121 gaagttttaa aaatgttgta ctcaggagaa tcctattaac gataacattt aagaggaaac
      181 gataatgagt aaaaaatcta tctttttagc cacgctcgct ttgatattta tctttggtca
      241 gcctgtcttt gccaactcaa gcgacgctac acaaacaaag ccttgtcatt gccatgagca
      301 caaaaaaata ctcagtcagc ttaatttaac tcaagagcaa aaaaccaaaa tcaaaacaat
      361 caaaatacaa gctcaaaaat caatcaaaga aaattataga caacttaaag cgatcaaaga
      421 acaaatcaat gcgctcattg caaatgagaa gcttgatgaa gccaagctgg atagtttaat
      481 caaccaaaga aataaaatag cagggactat gctaaaaaat cgtgtaatga tgaaaaatca
      541 aatttacaat cagctaacca acgaacaaaa atcacaatac aaaaagaata tgtaaaactt
      601 gattggtacc gataacaaaa aattcggtac caatctatcc caactcatca actataatca
      661 ttgctgtaat tatgatcttt ctcactgttg cttcctgaac aatattttct gaacgcaaaa
      721 aaatgggcaa acgttctctc taccattaag aaacagatga tatttcgctc agtatgctaa
      781 cccatagtat gctgcagcag ctgcactggc aatgatgcaa agtgcaaata ttatagttct
      841 cgccatattc ttctttgttt tgggtttcat aacaaatcct ggaatgacgg ttatcctgat
      901 tgatatttca tttaaattat agttcaaatt ttatgcatag ctagacccac atatggattg
      961 cccttacaca gaatagcatg gagctttact acaaattcag ttcaaaacac ttcctataat
     1021 cattgactta tgacctaatt tagaacaacc tatatcggcg atgagcaata ttgttattgc
     1081 agagtaaaaa agaaacccaa ttatgagagg acatatgatt tttttaagta ttcctaaagg
     1141 gatggaattt aaacaaatta cagaaaaaga caatacgaac gactattttg ttgatccgaa
     1201 tggaaagtta ccacgaatta atattcaggc tttagttaaa gacgccctgc agtacaacaa
     1261 aggtagaaaa aaagaaatct ccctg
//
LOCUS       DATNSN010000315         1264 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.319, whole genome shotgun sequence.
ACCESSION   DATNSN010000315 DATNSN010000000
VERSION     DATNSN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.319"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            87..>1264
                     /gene="sdbA"
                     /locus_tag="VAH76_003159"
     CDS             87..>1264
                     /gene="sdbA"
                     /locus_tag="VAH76_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbA"
                     /protein_id="HEO1387728.1"
                     /translation="MHKKYNYYSLEKEKKTFWQHILDILKAPFRLPGWVVSFFLARNI
                     THVALNPNNIPQQRLIHLTKTSNRPEDDIVVINFKKRPPHKWFNDTLIKIANTIAALP
                     FVTPRLRTRLHYDNENDINHVNKLLAEIDALVQGKSKQKYCKGRAFDWSKIHLKGLEF
                     LDPKMRGYVYEQLHEKYGYVSYTTKREPNIEFFTLKTPDGSELDSVQVTGEDEEKKPM
                     RERKFIITCIARDQNFINWIKDLNYTAKNLGATAISFNYRGVDYSRGLVWTENNLVDD
                     ILAQVQRLISLGADPKNICLDGMCIGGAVATIAAAKLHEKGMKVKLNNERSFTSLSSL
                     VFGFIVPELQTANWWSPLTYGRFLLAGVVYALLTPLIWLAGWPVDVTKAWNRIPAQDK
                     "
BASE COUNT          403 a          228 c          257 g          376 t
ORIGIN      
        1 ttttactatt tgtttgcata tatattaacc ctataggatt gcaatagata gtgccacttt
       61 gatttaatta atttttagaa gttaatatgc ataaaaaata caactactac agtcttgaaa
      121 aagaaaaaaa aacattctgg caacatatac tggatattct gaaggcccca tttaggctac
      181 ctggttgggt cgtttctttt ttcttggcac gaaatattac tcatgtggca ttaaatccta
      241 ataatattcc gcaacaacgt ttaatacatc ttactaaaac aagcaatcgt ccagaggatg
      301 atattgttgt cattaatttc aaaaaacggc cgcctcacaa atggtttaat gacacgctga
      361 ttaaaatagc caatacgatt gcggctcttc cttttgtaac accaagatta cgtacaagat
      421 tacattacga taatgagaat gacatcaatc atgtgaacaa gttgcttgcc gagatcgatg
      481 cgcttgttca gggcaaatca aaacaaaagt attgcaaagg cagggcattc gattggtcaa
      541 aaattcatct gaagggattg gagttcctgg accctaaaat gagagggtat gtttatgaac
      601 aactgcatga aaaatatggt tatgtctcat atacaacaaa acgcgaacct aatattgaat
      661 ttttcacctt aaaaactcct gatggatctg aattggattc agtgcaagtt acaggtgaag
      721 atgaagaaaa aaaaccaatg agggagcgca agtttattat tacctgtatt gcgcgggatc
      781 aaaatttcat taattggata aaggatttaa actatacggc aaaaaatctg ggtgccacag
      841 ccatcagttt caactacaga ggcgtggatt acagtcgcgg tcttgtctgg acagaaaata
      901 atcttgtcga cgatattctg gctcaggtgc aaaggctaat tagccttggt gccgacccta
      961 agaacatctg ccttgatggt atgtgtatag gtggggcggt agcaacaatt gccgcagcaa
     1021 aacttcatga gaagggcatg aaagtcaagt tgaataatga acgctctttt acttctcttt
     1081 cttctctagt atttggtttt attgtacctg aattgcaaac agcaaattgg tggagccctt
     1141 tgacttatgg tcgcttccta ctggctggtg tagtttatgc tttactcacg cctttaattt
     1201 ggctggcagg ctggccagtt gatgtgacca aagcctggaa tagaatacct gctcaggata
     1261 aaat
//
LOCUS       DATNSN010000316         1258 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.320, whole genome shotgun sequence.
ACCESSION   DATNSN010000316 DATNSN010000000
VERSION     DATNSN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.320"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..196)
                     /locus_tag="VAH76_003160"
     CDS             complement(<1..196)
                     /locus_tag="VAH76_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO1387729.1"
                     /translation="MSKTVNGVVKWFNEIKGFGFIEQEGGPDVFAHFKEISSSGFKTL
                     TEGQRVQFIVTQGAKGLQAQN"
     gene            375..722
                     /locus_tag="VAH76_003161"
                     /pseudo
     CDS             375..722
                     /locus_tag="VAH76_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(932..1243)
                     /locus_tag="VAH76_003162"
     CDS             complement(932..1243)
                     /locus_tag="VAH76_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387730.1"
                     /translation="MKKINFLLYLLAMLTISFATISHGASEDTDTTNPTKYIYRGSSI
                     TLEELATVADLSTARDQKGNPVTLEEESYQVGSFTRGTVTIIPKSGQTDDVITLDLKP
                     N"
BASE COUNT          384 a          215 c          233 g          426 t
ORIGIN      
        1 tattttgtgc ttgaagacct tttgctcctt gcgtcacaat gaattgtact cgctgacctt
       61 cagtcagggt tttaaagcca gaacttgaaa tttctttaaa gtgagcaaat acatccgggc
      121 cgccttcttg ctcaataaaa ccaaatcctt tgatctcgtt aaaccattta acgactccat
      181 ttactgtttt agacataagt atccttaagt atttattata atgcctgtat aggcgaattt
      241 agccggaaac cggtcaagaa ttaaaaacta ccgaacgagg gattaaatga ataaaacgac
      301 gatttgggga gtataaaata gacattttct agctgagagc agaatactat tctttaaggc
      361 tataagtcaa tcttattttg gctttactgc gtacgcgacc ggatagatta acacctcaac
      421 agcgacttaa gcgcgatgca ttccttgagg ataatccggc tataaaagct atataccagt
      481 tccagcaaca agttcatgca ttattcttac ataaagctat gaataaagat tggtgtaaaa
      541 aaatgattcc ccagttttta aatatgcttg atgacttaaa gaatagtccc tttaaagcct
      601 tggcagctct tggaaaaaca ttctgtcaat ggcaagaaga gattgttcgt atgtggcggt
      661 ttagaaaatc taatgacatt acagaaggct ttcacagaaa gatgaaattg agccatttat
      721 aatttattat ctctttattt tatatctctt tttttgaaaa tttgatggga tcgctcaggg
      781 cccgtcccca tttctttaaa gaatatcgaa ttcacgttta tttagattaa aaataaaaag
      841 ggataagaga cattttgggt tattttcttc ttgcttgctt tcataggata aaatgtgatt
      901 ttatcctatg tttagctaag gtgtttttga attaatttgg ttttaaatct aaggtaatga
      961 cgtcatctgt ttgcccactt ttgggaatga tagtcactgt acctcttgta aaagagccca
     1021 cttggtagct ttcttcttct aaggtgactg ggttgccttt ttgatcccgt gctgtggata
     1081 aatcagcaac agtcgctaat tcttcaagag taatagagga accacgataa atatattttg
     1141 ttgggtttgt agtgtctgta tcttcgctgg ctccatggct tatagtagca aaggatatag
     1201 taagcatagc taataaatac aatagaaaat ttatcttttt cattaggctg cccttaat
//
LOCUS       DATNSN010000317         1248 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.321, whole genome shotgun sequence.
ACCESSION   DATNSN010000317 DATNSN010000000
VERSION     DATNSN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.321"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            33..148
                     /gene="rrf"
                     /locus_tag="VAH76_003163"
     rRNA            33..148
                     /gene="rrf"
                     /locus_tag="VAH76_003163"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            254..329
                     /locus_tag="VAH76_003164"
     tRNA            254..329
                     /locus_tag="VAH76_003164"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Thr,seq:tgt)
     gene            402..486
                     /locus_tag="VAH76_003165"
     tRNA            402..486
                     /locus_tag="VAH76_003165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Tyr,seq:gta)
     gene            507..580
                     /locus_tag="VAH76_003166"
     tRNA            507..580
                     /locus_tag="VAH76_003166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Gly,seq:tcc)
     gene            633..708
                     /locus_tag="VAH76_003167"
     tRNA            633..708
                     /locus_tag="VAH76_003167"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:Thr,seq:ggt)
     gene            743..>1248
                     /locus_tag="VAH76_003168"
     CDS             743..>1248
                     /locus_tag="VAH76_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEO1387731.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTA
                     KAYDQIDAAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
                     YDFPGDDIP"
BASE COUNT          364 a          248 c          320 g          316 t
ORIGIN      
        1 atagcaatca aagcctcagg taaaccagtt ttcctggcga ctatagcgat ttggaaccac
       61 ctgataccat ctcgaactca gaagtgaaac atttccgcgc caatgatagt gtgaggcttc
      121 ctcatgcgaa agtaggtcat cgccagggtt ttattttata gtgcggcatg taaagccgac
      181 taaattaaaa taggttagct gtattcgcat aatataaata aataagcgct aacctatcat
      241 atgtaaagaa tatgctggcg tagctcagtt ggtagagcaa ctgacttgta atcagtaggt
      301 cccgggttcg attcctggtg ccagcaccat ctaaataggg ctaaacgttg actaaaaatg
      361 ttgacaatcc attcatctta aaagacaata gcgttctttt tggaggggtt cccgagcggt
      421 caaagggatc agactgtaaa tctgacggct cagccttcga aggttcgaat ccttccccct
      481 ccaccagtta agagagaagt taaaaagcgg gtgtagttca atggtagaac ttcagccttc
      541 caagctgata gcgtgggttc gattcccatc acccgctcca aagtagtgaa tagtatttgc
      601 ttgttggtgt gagttagcaa gctgatatga aagcccacat agctcaggcg gtagagcact
      661 tccttggtaa ggaagaggtc gttggttcga gtccagttgt gggcaccatg taatataaat
      721 tagcaaacgg ggagactttc cgatggcgaa ggaaaaattt gaacgtaaga agccgcacgt
      781 aaacgtgggc acgattggtc acgtagacca tggcaagacg acattgacag cagctattac
      841 aacgattatg gcgaagaaat atggtggtac agcgaaggcg tacgatcaaa ttgatgctgc
      901 gccagaagaa agagagcgtg gtattactat ttctacagcg cacgttgagt acgaatcagc
      961 gagcagacat tatgcgcacg tagactgccc ggggcatgct gactatgtta agaacatgat
     1021 tactggtgct gcgcaaatgg acggagcgat actggttgta tcagcggctg atggtcctat
     1081 gccacaaacg agggaacaca ttctattgtc tcgccaggta ggtgttccat atattgttgt
     1141 gttcatgaac aaagcggata tggttgatga ccctgagtta ttagagttag tggaaatgga
     1201 agtgcgagat ttattaagca gttacgattt cccaggggat gacatacc
//
LOCUS       DATNSN010000318         1246 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.322, whole genome shotgun sequence.
ACCESSION   DATNSN010000318 DATNSN010000000
VERSION     DATNSN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.322"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            254..>1246
                     /locus_tag="VAH76_003169"
     CDS             254..>1246
                     /locus_tag="VAH76_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387732.1"
                     /translation="MGGLTSEQYHSQVVGKIGYIARCMQTIDPENNLKKIREDYQDVL
                     IWAEKNYRFEEILEASKSGKCPNDLDALSRRSLILQELLRLVSSISPFKMKLDLIESQ
                     YEKMKQHVNLWKSDYHVKLNQLNQLTDYLKNAAPTPKNHFLRAMTSALQMQIAQYGIT
                     EDNEGINQLFKLGLHLLAMANEKIDEQYHLFKRYVKDQPEESPFEGILPVEDQKILVK
                     AMIDYAVPKLSLKVLQDKLSAVSSSDALTKTLLDSIDRIVEENEKLNALSKVKLGKFS
                     LDIREIEEIYSQALKISPQDALLYTAQQCDAKLLSMAFPDSQNYIVESISNKEAK"
BASE COUNT          478 a          193 c          210 g          365 t
ORIGIN      
        1 ataaaaactt tatccatgta gttaattata ttaactataa aaactgtcct attaaattga
       61 aaatctatat gaaaaaactg aaaaaaacag gcttcattat ttgaattaaa tatttgaaaa
      121 cagtaatttt tcttaacaaa attcatacat aatttactaa atggaaactt ttacatggca
      181 acaccttgag ttttttaccc attattgata ctatttgggc aattaattat cttttatcaa
      241 ggtgggtata aaaatgggtg gcttaactag cgagcaatat catagtcaag tagtcggcaa
      301 aattggatat atcgccagat gcatgcaaac tatagatcct gaaaataatt tgaaaaagat
      361 aagagaagat taccaagatg tattgatatg ggcagagaaa aattatcgtt ttgaagaaat
      421 attggaagcc agcaaatctg gaaagtgccc aaatgatctg gatgcattaa gtcgacgatc
      481 gttaatactc caagaattgc tacggcttgt ttcatcaatt agtcccttta aaatgaaatt
      541 agacctgatt gagtctcaat atgagaaaat gaaacaacat gtgaatttat ggaaaagtga
      601 ttaccatgta aaactaaatc aattgaatca attaacagac tacctcaaaa atgcagcacc
      661 aactcctaaa aatcattttc taagagcaat gacctctgca ttgcaaatgc agatagccca
      721 atatgggatt acagaagata atgagggtat caaccagttg tttaaactgg gattgcattt
      781 actcgcaatg gctaacgaaa aaatagatga acaatatcat ctgtttaaaa ggtatgtaaa
      841 agatcagcct gaagaatctc cttttgaggg cattttgcct gtggaagacc agaaaatttt
      901 agtcaaagct atgatagatt atgccgtgcc gaaactttca cttaaggttt tacaagataa
      961 attatcagca gtatccagtt ctgatgcatt aacaaaaaca ttacttgata gcatcgacag
     1021 aattgttgaa gaaaacgaaa aattaaatgc tttaagcaaa gtcaaattag gtaaatttag
     1081 cctggatatc agggaaattg aagagatata ctctcaagct ctgaaaatat cgccacaaga
     1141 tgctttgcta tatactgctc aacaatgtga tgccaaatta ttaagcatgg catttcctga
     1201 ttcccaaaat tacattgttg aatctatatc gaacaaagag gccaag
//
LOCUS       DATNSN010000319         1232 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.323, whole genome shotgun sequence.
ACCESSION   DATNSN010000319 DATNSN010000000
VERSION     DATNSN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.323"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            78..434
                     /locus_tag="VAH76_003170"
     CDS             78..434
                     /locus_tag="VAH76_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387733.1"
                     /translation="MSKFFTLLICISIGLLSFPTYAQKTIRLSPNETKTLKNSTLWTL
                     NATCNVQSGTQVNGKIKIIVLKNNGKVNGKSLSTGQGTSITIKNNSSISVSAESGTQI
                     NLINLGNQELQAVCST"
     gene            complement(498..1220)
                     /locus_tag="VAH76_003171"
     CDS             complement(498..1220)
                     /locus_tag="VAH76_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEO1387734.1"
                     /translation="MNYQYSPSLKKKLLLAFTTLFIVQMFYFAPVRASTRDTQLWTLM
                     NAIGPINKQHRKIRYWLEIQDRIGENISQLSQLILRPGIGYELLPTTTIWLGYDRIYT
                     AQPFANPPVNENRVWQQLLWSKEYTRLRLTARSRLEERFIQRAIHTAWRYRQLFKTSI
                     FIPKHPKYAAVISDEAFFHLNDFNLQRNQGFDQNRLFVGLGYRTSKNSTIEIGYLNQM
                     IKRVNNPDYRGDYLSMALLLTE"
BASE COUNT          391 a          206 c          242 g          393 t
ORIGIN      
        1 cttaagcaaa actgtatata atgtatacat attgcattaa aatagctggg ccttgggttc
       61 tggagaagga aagtagcatg tctaaatttt ttactttgtt gatatgtatc tcaattggtt
      121 tattaagctt cccaacttat gctcaaaaaa cgatacgact tagccctaac gaaaccaaga
      181 cgctaaaaaa tagcacttta tggactttga atgcgacctg taacgtacag agcggcacgc
      241 aggttaatgg taaaataaaa atcattgttt taaagaacaa tggcaaagta aatggtaaaa
      301 gcctttcaac cggccaggga acttctatta caataaaaaa taacagcagc atctcggtta
      361 gtgcagaatc aggaactcag attaatctga ttaacttggg taatcaagaa ttgcaagcag
      421 tttgttctac ttaaattaaa tcaatttttc tcaaacaaac cattgaaagt aatggtttgt
      481 tttctttgtc cgcttgttta ttctgttaat aatagagcca tggataaata atctccgcga
      541 tagtcagggt tatttacgcg tttaatcatt tgattgagat acccaatctc tatagtgctg
      601 tttttactgg ttctataacc taagccaaca aataaacggt tttgatcaaa gccttgatta
      661 cgttgaaggt tgaagtcatt cagatgaaaa aaagcttcat cactaatcac ggctgcgtat
      721 ttagggtgtt tgggaataaa aatactggtt ttaaataatt gtctataccg ccaggcggta
      781 tgaatggctc tttggataaa gcgctcttcc aaacgtgaac gagcagtaag tcttaagcga
      841 gtatattcct tgctccacaa taattgttgc caaactcgat tttcgtttac aggagggttg
      901 gcaaatggtt gcgcagtata aattctatca tatccgagcc agatggttgt tgtaggcaag
      961 agttcatatc ctatgccagg gcgtaatatc agttgggaca attgcgaaat attttcacca
     1021 attctgtctt gtatctcaag ccaatatctt attttgcgat gctgcttatt gattggtcct
     1081 atcgcattca taagtgtcca taattgagta tcccttgttg atgctctgac aggggcaaaa
     1141 tagaacattt gtacaataaa taaagtagta aatgcgagca ggagtttttt ctttaggctg
     1201 ggggaatatt gataattcat tgtttaaaaa ga
//
LOCUS       DATNSN010000320         1211 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.324, whole genome shotgun sequence.
ACCESSION   DATNSN010000320 DATNSN010000000
VERSION     DATNSN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.324"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..180
                     /locus_tag="VAH76_003172"
     CDS             <1..180
                     /locus_tag="VAH76_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEO1387735.1"
                     /translation="TPSEVAELYARDEMAQIRSSNRFYLFFKLFRPKLINWLEKQNDL
                     EPHVTYRFSGQSSSA"
     gene            342..1085
                     /locus_tag="VAH76_003173"
     CDS             342..1085
                     /locus_tag="VAH76_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO1387736.1"
                     /translation="MVRPRIYLCSLLLVIPFYSGYALNLVIGTLSYNPPFEIQVNNPS
                     KENEFYGFEIELMDEICERIHAKCQYKPLLFEQILAEVNSGAINLGIGSINITPERSE
                     RFLFSLPYKSSYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTVDYLKVYFNNNIQFKY
                     YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDGFPFGIGFGVVARLDQA
                     ELIEKINQALIEIENDGTYLKIYNLFFQP"
BASE COUNT          401 a          218 c          205 g          387 t
ORIGIN      
        1 acaccatccg aggttgcaga actgtacgca agggatgaaa tggcacaaat tcgttcaagc
       61 aaccggttct atttgttttt taaattattc cggccaaaat taatcaattg gttagagaag
      121 caaaatgacc tggagcctca tgtgacttat cgtttctcag ggcaaagttc ttctgcttga
      181 taccactaac ttaacaaacc cggtacaact ggcaatttac cttgctctgc tttatgcaag
      241 gtaaagaccc ctgatttatg aaaaaatata aatccaaccg ttcttttatt tttcaaaaat
      301 aaggctaaaa taataaataa ctatataaaa aggatattta aatggttcgc cccagaattt
      361 atttatgttc tttgctactg gttattccct tttatagtgg gtatgctctt aatttggtca
      421 tagggacctt gtcttataat cctccttttg aaattcaggt caacaaccct tccaaggaaa
      481 atgagtttta tggttttgaa attgaactca tggatgagat ttgcgaacgc atccatgcaa
      541 aatgccaata caagccattg ttatttgaac aaattttagc tgaagttaat tctggtgcaa
      601 tcaatttagg cattggcagc attaatatta cgcctgaaag aagcgaacga tttttattca
      661 gccttcccta caaatccagc tatcaccagt ttcttatacc atctaactcg aatataaaaa
      721 aaattgacga tctcaaagga aagactatag gaattcactt aggttcacct actgtcgatt
      781 atttaaaggt ctactttaac aataatattc aatttaaata ttacgaaaca tttatggatt
      841 tacttaatgg gctgactgat ccgcaagtta gcgcaatagt gactaatagc gatcaagcgc
      901 aatactgggc atcaagtaca ccggactgta agttattggg cgacggtttt ccttttggaa
      961 taggatttgg agttgtcgcg agattagatc aagcggaact gattgaaaaa attaatcagg
     1021 cattaattga gattgaaaat gatgggactt accttaaaat atataacctc ttctttcaac
     1081 cttaagtatt atttcgaccg acaaaggact ccagtgctta ataaatctat tgatttttgt
     1141 ctataatctg agaaacaata aaaggttaac atcattctat caatactata aaaaatgact
     1201 ctcggctatt t
//
LOCUS       DATNSN010000321         1190 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.325, whole genome shotgun sequence.
ACCESSION   DATNSN010000321 DATNSN010000000
VERSION     DATNSN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.325"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>1190)
                     /locus_tag="VAH76_003174"
     CDS             complement(<1..>1190)
                     /locus_tag="VAH76_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HEO1387737.1"
                     /translation="KLPVNPDNLFNGSECFSLDGLLKLIDKSWGYENKTVDEISLCLE
                     FPHGLLTRSSKWLSQIKRWSAKLLGIEELISQTINDGSYCVLLHHARLSLMLGDHYFS
                     SLDVNQTSNWKCTTELIANTQKDKSPKQALDQHLVGVSESARDVVAKLPLIENGLEFS
                     FNTAELKRKSPPAYTWQDTAAKKVRDWRLSCNDMRKGFFAVNIASTGCGKTFANAKVM
                     MALSEDGDSLRYVLALGLRTLTLQTGDEYRERIFNKNDGTDLAVLIGSKAIADLHYQS
                     RLDQTYEQEQQGSESIESLLADSDEIVYQGELPEEGLTTILKKHKDRQLLYAPVLVCT
                     IDHMIAATETTRGGRYILPSLRLLSSDLVIDEVDDFTGSDLIAIGRLIHLAGMLGRKV
                     MISSA"
BASE COUNT          331 a          277 c          250 g          332 t
ORIGIN      
        1 gccgaagaaa tcatcacctt gcgccccagc atacctgcca gatgaatcag acgacctatg
       61 gcgattaaat cgctgcccgt aaagtcatct acttcatcaa tgaccaagtc agacgatagc
      121 aaacgcaggg agggcaggat gtagcgtccg ccacgagtgg tttccgttgc agcaatcatg
      181 tgatcaatag tgcaaaccag aacaggagca taaagtagct gcctgtcttt gtgtttcttt
      241 aagatagtgg ttaaaccttc ctcaggcagt tcaccctgat aaacaatttc atcggagtct
      301 gccaacaaag actcaatgga ctctgaacct tgttgttctt gttcatatgt ctgatccaat
      361 ctgctttgat agtgcaagtc agcaatggct ttagagccaa tcaataccgc cagatcagta
      421 ccatcatttt tattgaatat gcgttcacgg tactcgtctc ctgtttgcaa tgtcagtgtg
      481 cgtaaaccaa gggctaggac atagcggaga ctatcaccat cttctgaaag cgccatcatc
      541 actttggcat tggcaaatgt tttaccgcat ccggtactag cgatgttcac tgcaaaaaag
      601 ccttttcgca tgtcgttaca ggacaagcgc caatcacgta cttttttagc ggcagtatcc
      661 tgccaggtgt aggccggggg acttttgcgt tttaactcag ctgtattgaa actgaattcc
      721 aatccgtttt cgataagtgg caatttcgct acaacatccc tcgcactctc agaaacgcca
      781 accaggtgct gatccaaagc ctgctttggc gatttgtcct tctgagtatt ggcaatcaat
      841 tctgtagtac atttccaatt agaagtttgg ttgacatcca aggaagaaaa atagtgatcg
      901 cctaacatca gtgataagcg agcatggtga agcaatacac agtaactgcc atcattgatg
      961 gtttgagaaa ttaattcttc tatacccaat aacttggctg accaccgctt gatttgtgaa
     1021 agccatttac tggagcgagt taacaatcca tgggggaact ccaaacaaag gctaatttca
     1081 tcaacggtct tattttcata tccccagctt ttgtcaatga gtttcagcaa tccgtcaaga
     1141 ctgaagcatt cacttccatt gaataggttg tcagggttaa caggtaattt
//
LOCUS       DATNSN010000322         1185 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.326, whole genome shotgun sequence.
ACCESSION   DATNSN010000322 DATNSN010000000
VERSION     DATNSN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.326"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1185
                     /locus_tag="VAH76_003175"
     CDS             <1..>1185
                     /locus_tag="VAH76_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEO1387738.1"
                     /translation="YANWNINQKRAYKDLKEVAINLSNNIDGFIEDLLQEIYALPVYG
                     KKIVDCKSDLYPFLEHITLNNPKISGVIISDNKHNLFCSTLPNNKDLIATPIRSRSII
                     GPYNLPLFDQPVYLIQQKMGNYLIGILVIASVLKSELQTDKNQSTSIALYNEYEKKNI
                     FRIRYTEQNKNWVLSNTQETDTQYTQLGLVAIEKLQSINGVSVVVSENNKTILHNFWY
                     SQTITILIILIFSFILYVLIKNMMTKRYSLQGAIKLAIKNEEFYPVYQPLFDCDEERF
                     TGAEVLLRWENEDSQIIMPDFFIAEAEATGLIVPITLQIIEIAFKEFRSLFEERSYFH
                     LAFNISALHFTDSVFFNHFHKLIEKYSISPHQIIFEITERELLDKNDTTFINKMQELR
                     QA"
BASE COUNT          429 a          207 c          165 g          384 t
ORIGIN      
        1 tttacgctaa ttggaatatt aaccaaaaaa gagcttataa agatttaaaa gaagttgcaa
       61 tcaatctcag taacaacata gatggtttta tcgaagatct acttcaggaa atctatgctt
      121 tgcctgtata tgggaaaaaa attgtcgatt gtaaatcaga tttatatcca ttcttggaac
      181 atatcactct aaataatcct aaaatctctg gtgttattat tagtgacaac aaacacaatc
      241 tattttgttc aactttaccc aataataaag atcttattgc aacaccgatt cgttcacgtt
      301 ctattatagg cccctataat ttaccattat ttgaccagcc cgtttacctg atccagcaaa
      361 aaatgggcaa ttaccttata ggtattttag ttattgcttc tgtcttaaaa agtgaattac
      421 aaacagataa aaatcaatcc acctctatag ccctatataa tgaatatgag aaaaaaaata
      481 tctttcgtat tcgatatact gagcaaaata aaaactgggt attaagcaat acgcaagaaa
      541 ctgacacaca atatacccaa ttaggtctcg ttgctataga aaaactacaa agcataaatg
      601 gtgtttctgt cgttgtttct gaaaacaata aaactatcct tcataacttt tggtatagtc
      661 aaacaataac aattcttatt attttgattt tttcctttat tttgtatgtc ctcataaaaa
      721 acatgatgac caaacgttat tcattacaag gcgcaataaa actggctatt aaaaatgagg
      781 aattttatcc tgtataccaa cctttgtttg actgtgatga agaacgattc acaggggctg
      841 aagtattatt gcgatgggaa aatgaagaca gtcaaatcat tatgcctgat ttttttattg
      901 ctgaagcaga agctaccggg ttaattgtgc caattacact tcaaatcata gaaattgcct
      961 tcaaagaatt tagatctctg tttgaagaac gatcttactt tcaccttgca tttaacattt
     1021 ctgcactaca tttcacagat tcagtttttt tcaatcattt ccataagcta atagaaaaat
     1081 atagtatttc accgcatcaa ataatctttg aaattaccga aagagaactc ctcgataaaa
     1141 acgacacaac ttttatcaat aaaatgcaag aattaaggca agctg
//
LOCUS       DATNSN010000323         1184 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.327, whole genome shotgun sequence.
ACCESSION   DATNSN010000323 DATNSN010000000
VERSION     DATNSN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.327"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            74..>1184
                     /locus_tag="VAH76_003176"
     CDS             74..>1184
                     /locus_tag="VAH76_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012518412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEO1387739.1"
                     /translation="MSFVENFSILKDPRSEINQRHNLLDILFLAGTAVMSGAEGWKDI
                     KDFGEEKLDWLRQYRDFKHGIPADDTIARVISALDSEQFTLCFVNWVNELRSKDGREQ
                     IAIDGKTLRRSYTGDRTTALHSITAWSKRNGLILAQMRSNGKANEKASVLEMLDLLEL
                     KGAIVTADAMNTEKKIAKKIHDKGGDYVLCVKNNHKKLLNEIKDYFHIMERDHREIWS
                     QNYFEEVDAGHGRIETRTCRQIAISPWVEGYADWQGLQTVIEVTRTREIKEKTGTEVQ
                     YYISSLPLEPKQIADAIREHWAVENKAHWVLDVTYKEDDSRVRRDNAAENLALLRRFA
                     LNLARLHPQKNSMKGKLKKAMWSDKFRSELLIGQIQ"
BASE COUNT          413 a          198 c          273 g          300 t
ORIGIN      
        1 tgaccagtaa ttatcattta gtatgatttt tcagtgattt gtgatcaaat actcattttt
       61 tggatgacaa gaaatgagtt ttgtagaaaa tttttccata cttaaagatc ctcgtagcga
      121 aataaatcag cgccataatc tgctagatat cttattttta gcaggtactg cggtgatgtc
      181 tggagctgag ggatggaaag acataaagga ttttggagaa gaaaagcttg attggctaag
      241 acaatatcgg gattttaagc atgggatacc ggccgatgat accatagctc gtgtaattag
      301 tgctcttgat tcagagcagt tcacgttgtg ttttgtaaat tgggtgaatg aacttcgttc
      361 gaaagatggt cgagaacaaa tagcgataga tggaaaaact ctacgccgct catatacagg
      421 tgaccgcacc acagccttac atagcattac tgcatggagt aaacgaaatg ggttgatatt
      481 agctcaaatg cgctctaatg gaaaagcaaa cgaaaaagcc agtgtgttag aaatgctaga
      541 cctcttggag cttaagggag caattgttac tgccgatgct atgaatacag aaaagaagat
      601 agctaaaaaa atacatgata aaggcggaga ctatgtgctt tgtgttaaaa acaaccataa
      661 aaaactgcta aatgaaatta aggattattt tcatatcatg gaaagagacc atcgagaaat
      721 ctggtcacaa aattacttcg aagaagtgga cgcaggccat gggcgcatag aaacaagaac
      781 atgccggcaa atcgcaatta gcccatgggt tgaaggttat gccgactggc aaggattaca
      841 aaccgtaatt gaagtaacgc gaacacgtga aataaaagaa aaaaccggta ctgaagtgca
      901 atactatatc agttccttgc cgttagaacc caaacaaatt gcagatgcta tcagagagca
      961 ttgggcagtc gaaaacaaag ctcactgggt gttggatgtg acctataaag aagatgactc
     1021 aagagtacga agagacaacg cagcagaaaa tctggccttg cttcgtaggt ttgcactcaa
     1081 tctggctcga cttcatcccc agaaaaatag catgaaggga aaattaaaaa aggctatgtg
     1141 gtctgataaa tttagaagtg aactcttgat tgggcaaatt cagg
//
LOCUS       DATNSN010000324         1170 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.328, whole genome shotgun sequence.
ACCESSION   DATNSN010000324 DATNSN010000000
VERSION     DATNSN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.328"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..650
                     /locus_tag="VAH76_003177"
     CDS             <1..650
                     /locus_tag="VAH76_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEO1387740.1"
                     /translation="QLDFGLFLFENTYLIAFFLFFAVIWAINHFNFMDGLDGFCASQA
                     IFLLAAYALFFNSAGSLIYQDFCLILISSLAGFLIFNFPPAKIFMGDVGSASLGLITF
                     FIAIIAQKNYQIPILYWFILNSLFLFDSTVTLIRRIIKHEKWFSPHKKHAYQRLKQLG
                     INSRFILLGQMLLNCSFLSIILLHRIIGFNLNYLMLLQLICLLFLYIIVEKKYPM"
     gene            765..>1170
                     /locus_tag="VAH76_003178"
     CDS             765..>1170
                     /locus_tag="VAH76_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEO1387741.1"
                     /translation="MKSKHIIISCAVLIGSLFSYKIYGGKCDYIISSIPFVIKHSGKW
                     CLNQNMYTPISDYDAIIIQANNVILDGNGYLLKGPGTSTTLSRGIVAYNRQNIIVKNI
                     IIDGFYIGILFADNSLPSDTLIPINYFISNSIY"
BASE COUNT          352 a          172 c          176 g          470 t
ORIGIN      
        1 cccagttaga ttttggttta tttctatttg aaaacaccta tttaattgct ttttttctat
       61 tttttgcagt tatttgggca attaatcatt ttaatttcat ggatgggctt gatggttttt
      121 gcgcttccca agcgattttt ttgttagccg catatgcttt gtttttcaac tcagcaggtt
      181 cattaattta tcaagatttt tgcctgattc ttatttctag tttggcaggg tttttaattt
      241 ttaatttccc accagccaag atatttatgg gagatgtagg gagcgcctcc ttaggattaa
      301 ttactttttt catagcaatc attgctcaaa agaattatca aattccaatt ctatattggt
      361 ttatcctgaa ctcattattt cttttcgatt ctacagtgac tttaattcga aggattataa
      421 agcatgaaaa atggtttagt ccccataaaa agcacgctta tcagagatta aagcaattgg
      481 gcattaactc gaggtttatt ttattaggtc aaatgttatt aaattgcagt tttttatcaa
      541 ttattctatt gcatcggata attggtttta atctcaatta tctcatgttg ctccagctaa
      601 tttgtttatt attcctatac ataatagtag aaaaaaaata tcccatgtaa cgaactgtat
      661 aaattaaata ttgcaatcca ttatttttct atcagatact aatcttattt agatgcttaa
      721 aatcctaatt gatttaagct taagggcttc taagggcacg agatatgaaa agtaaacata
      781 taataatttc ttgtgcagta ttaattggaa gtttattttc atataagatt tatggtggaa
      841 aatgtgatta tatcatatct tccattcctt tcgttattaa acattctggg aaatggtgct
      901 taaatcaaaa tatgtacacc ccaatttctg attatgatgc gattattatt caggctaata
      961 atgttatatt agatgggaat ggttatcttt taaaggggcc tgggacctca actaccttat
     1021 ctagaggtat tgttgcttat aacagacaaa atattatagt caaaaatatc attattgatg
     1081 gtttttatat tggaattcta tttgctgata atagccttcc gtctgacact cttattccaa
     1141 ttaattattt tatctcaaat agtatctaca
//
LOCUS       DATNSN010000325         1158 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.329, whole genome shotgun sequence.
ACCESSION   DATNSN010000325 DATNSN010000000
VERSION     DATNSN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.329"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            52..1139
                     /locus_tag="VAH76_003179"
     CDS             join(52..313,313..1139)
                     /locus_tag="VAH76_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611583.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO1387742.1"
                     /translation="MKKSKFSDSQILSILKQAQSGVAVPDLCREHGISNATFYNWRAK
                     YGGMDLPMMTRLKELEAENSRLKKMYAEERLKSEILKEVLEKKLKKPSARRVLARVIV
                     QERHIAVRMACWLFSISETCYRYEPKLNEENRVIADWLVRLTQNNRNWGFGLCFLYLR
                     NVKGFCWNHKRVYRVYKELELNLRIKPKKRLIREKPEPLVVPTSSNQCWSMDFMHDQL
                     DNGRCYRLLNIIDDFNREGLTIEADFSLPSARVIRTLEQVIEWRGKPKQIRCDNGPEY
                     VSHKLARWAKKHDIELVFIQPGCPQQNAYIERYNRTVRYDWLNQYLFESIEQVQEQAT
                     KWLWSYNNERPNSAIGGIPPKRKLALAA"
BASE COUNT          359 a          222 c          279 g          298 t
ORIGIN      
        1 ttgctgccaa aagtagaatt ttctcgtaat gtatatgcaa ggagattctg catgaagaaa
       61 tcaaaattta gtgacagcca aatcctgtcg atattgaagc aagctcaaag tggtgttgca
      121 gtacccgact tatgtcgaga gcatggtata agcaatgcga cattctataa ctggcgggcc
      181 aaatatggag gcatggatct tccaatgatg acacggctaa aggaacttga agcagagaat
      241 agccgcttaa agaagatgta cgctgaagag cgcttaaagt cagaaatttt aaaagaggtc
      301 cttgaaaaaa agtaaaaaag ccatccgctc gtcgagtgct tgcacgcgtg atagtgcagg
      361 aacggcatat tgcagtcagg atggcttgct ggctatttag catcagtgag acgtgctacc
      421 gatatgaacc caaactgaat gaagaaaaca gggtcatagc ggattggctg gtgcgcttaa
      481 ctcaaaacaa ccgtaactgg ggttttggct tgtgtttttt atatttgcgc aacgtgaaag
      541 gtttttgttg gaatcacaaa cgtgtttacc gtgtatacaa agagctcgag ttgaatttac
      601 ggataaaacc taaaaaacgg ttaattaggg aaaagcctga gcccctggtc gtgccaacat
      661 caagcaatca gtgctggtca atggatttta tgcatgatca actggataat ggacgctgtt
      721 atcggttatt gaatattatt gatgacttta accgagaagg cctgacaatc gaagctgatt
      781 tttcattgcc ctctgctcga gtcatccgga cactggagca agtgattgaa tggcgaggca
      841 agcctaagca gatacgttgt gataacgggc ctgaatacgt aagccataag ctggcaagat
      901 gggctaaaaa acacgatatt gagcttgtat tcatacagcc aggttgtccg cagcaaaacg
      961 cctatattga gcgctataat cgtactgttc ggtatgactg gcttaatcaa tacttatttg
     1021 agagtatcga acaagtccaa gaacaggcca ccaagtggct ttggtcttac aacaatgaac
     1081 gtcctaactc ggcaattgga ggtataccac ccaaaagaaa attggctcta gcagcctagg
     1141 tttctacttt tagcgtca
//
LOCUS       DATNSN010000326         1149 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.330, whole genome shotgun sequence.
ACCESSION   DATNSN010000326 DATNSN010000000
VERSION     DATNSN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.330"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..781
                     /locus_tag="VAH76_003180"
     CDS             <1..781
                     /locus_tag="VAH76_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387743.1"
                     /translation="NASNYFFISGFVISKIKYLPIAVVSSALNLISLLFYLVGYSLWF
                     IASHFYPGQDKKMQEWYEFAQFKEQYLYAAALGLVATSISMMAIFSPIMLVLSGWLFF
                     GSNIVWTIGEYNKLNNPPSHEENFSKDRQNAYVSYALSISLIGFITAVSTTVAFFIPV
                     MTVPLLIVTTIVCIGVGSLAFEYWIESKFGGSQPDITIDESYKQMSNDLGEKITLEPN
                     PTPEPYHGTKPLHFTPHDVKMKEPSPDLQMDLSSRTCKPQY"
     gene            820..>1149
                     /locus_tag="VAH76_003181"
     CDS             820..>1149
                     /locus_tag="VAH76_003181"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO1387744.1"
                     /translation="MDNLTSNMISEAHKVLVNAYAPYSKFSVAACICTDKNNFYTGVN
                     VENISYGLTTCAEVSAICNMVTAGERKIKSIVVLAANNLLCSPCGACRQRIHEFSSSD
                     TMVHLCNK"
BASE COUNT          338 a          218 c          215 g          378 t
ORIGIN      
        1 taacgcaagt aattatttct ttatatctgg atttgtaatt tcaaaaatta aataccttcc
       61 cattgccgtg gtttcttccg ctctgaatct gatttcactt ttgttttacc tggttggcta
      121 tagcttgtgg tttattgcaa gtcattttta tccaggccaa gataaaaaaa tgcaagagtg
      181 gtatgagttt gctcaattta aagaacaata tctttacgct gcagctctag ggctcgtcgc
      241 aacatctatc agtatgatgg caatcttttc cccaataatg cttgttcttt caggatggct
      301 gttttttggc agtaatattg tttggacaat tggtgagtac aacaaattaa ataatccacc
      361 cagccatgaa gaaaattttt ccaaagatcg tcaaaatgcc tatgtgtctt atgctttatc
      421 cataagttta ataggattca ttacggcagt ctcaactact gttgctttct ttattccagt
      481 aatgacggta cccttgctta tagttacaac tattgtatgt attggggtag gatcgttagc
      541 gtttgaatat tggatcgaat cgaaatttgg cggctctcag cctgatatca ctatcgatga
      601 aagctacaaa caaatgagca atgatttggg ggaaaaaatt actttggagc ctaatcctac
      661 cccagaacct tatcatggaa ccaagccgct tcactttacg ccgcatgatg tcaaaatgaa
      721 agaaccgtct ccagatcttc aaatggattt atcctctcgt acttgtaaac cacagtatta
      781 atttagtata ttctgcacgc caatgatgaa ggtgtgacaa tggataattt aacttcgaat
      841 atgattagcg aagcgcataa ggtactagtc aacgcttatg ccccctattc caaattttca
      901 gtagctgcct gtatatgcac tgataaaaat aatttttata caggggtgaa tgtagaaaat
      961 atttcttatg ggctaacaac ctgtgctgaa gtttcagcga tttgcaatat ggttaccgca
     1021 ggtgagcgaa aaattaaaag cattgtggta ttagctgcaa acaatttgct atgctctcct
     1081 tgtggcgcat gccgacaaag aattcatgag ttttcatcct ctgataccat ggtacatctc
     1141 tgtaataaa
//
LOCUS       DATNSN010000327         1112 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.331, whole genome shotgun sequence.
ACCESSION   DATNSN010000327 DATNSN010000000
VERSION     DATNSN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.331"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            65..229
                     /locus_tag="VAH76_003182"
     CDS             65..229
                     /locus_tag="VAH76_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387745.1"
                     /translation="MNKENNLNDVIAEKLEDLMVPGFITEVTPIEADIMGAFSEDALS
                     EIDAQEAAYD"
     gene            222..>1112
                     /locus_tag="VAH76_003183"
     CDS             222..>1112
                     /locus_tag="VAH76_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HEO1387746.1"
                     /translation="MTKLSHHQIVANQIIESLKLGTAPWLKPWEPGIGNGQIPFNPVT
                     GKRYRGINALYLMLNQTEDNRWLTYKQAQSINAQVRKGEKGTTVQYWKFSEEKIKKDD
                     SGNPLLDEQGNPVKVQVNLERPKVFYARVFHASQIDNMPELVQKEQDWSLIDRAETLL
                     LNSGATIFHSEADRAFYRLSTDSIHLPPKEQFKSAAHYYATALHELGHWSGHPLRLNR
                     DLSHPFGSEGYAKEELRAEIASMLLGAELGIGHDPSQHTAYIKSWAQILEDDPLEIFR
                     ASADAEKIVNHICALEQVQDL"
BASE COUNT          358 a          241 c          248 g          265 t
ORIGIN      
        1 cacacggggc aaatcaaggg aatgatcaca cccgtatggg tcaaagcaac aatggagaaa
       61 gtgtatgaat aaagaaaaca atctaaacga tgtgattgca gaaaaattag aggacttgat
      121 ggtccctggt tttatcactg aggtcacacc catcgaggcg gacatcatgg gtgctttttc
      181 tgaggacgcg ttaagcgaaa ttgatgctca ggaggcagcc tatgactaag ttgtctcacc
      241 accagattgt cgccaatcaa atcattgaaa gcttgaaact tggaacagct ccctggttaa
      301 agccatggga gcctggtatc ggtaatggcc aaataccttt taatcctgtc acaggaaagc
      361 gttatcgagg catcaatgcc ttgtatttga tgctgaatca aactgaagat aatcgctggt
      421 taacctacaa acaggcccaa agcatcaatg cacaggtccg caaaggcgag aaaggaacaa
      481 cagtgcaata ctggaaattt agtgaagaaa aaatcaaaaa ggatgattca ggcaatccgc
      541 ttcttgatga gcaaggcaat ccagtcaaag tgcaagtcaa ccttgaaaga cccaaagtgt
      601 tttatgccag ggtatttcac gcctcacaaa ttgataatat gccggaactc gttcaaaaag
      661 agcaggactg gtctttaatc gatagagctg aaactttgct actaaattct ggcgcgacca
      721 tttttcattc agaagcagac agagcatttt acagactatc gacagatagt attcatcttc
      781 ctcccaagga gcaatttaaa tctgccgccc attattatgc cacagccctt cacgaactag
      841 gtcactggag tggccatcct ttaagactta atcgagacct aagtcatcca tttggcagtg
      901 aaggatatgc caaagaagaa ttaagagcag aaattgccag catgctgttg ggcgcagagc
      961 ttggcattgg tcacgaccct tctcaacaca cggcttacat caagtcatgg gctcaaattt
     1021 tagaagatga tccactggaa atattcagag cctcagccga tgcggaaaaa atcgtcaatc
     1081 atatttgcgc tttggaacag gtccaggact ta
//
LOCUS       DATNSN010000328         1109 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.332, whole genome shotgun sequence.
ACCESSION   DATNSN010000328 DATNSN010000000
VERSION     DATNSN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.332"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1009
                     /gene="proB"
                     /locus_tag="VAH76_003184"
     CDS             <1..1009
                     /gene="proB"
                     /locus_tag="VAH76_003184"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO1387747.1"
                     /translation="SVLNNLVNQIIYLKNLGHQVILVSSGAVGSGRKIAKNSLGKTYG
                     DTVGEKQLLASIGQPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTEL
                     LKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHPND
                     PESKLINTIDPNSGWPNVSAVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIV
                     PKILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRNGNRVMSILP
                     IGIEDFRGECKKGDLIDILNSKNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDHLHI
                     L"
BASE COUNT          393 a          180 c          192 g          344 t
ORIGIN      
        1 ttccgtacta aataatcttg taaatcaaat tatttattta aagaacctgg ggcatcaggt
       61 cattttagta agctctgggg ctgtgggctc tggtcgaaaa atagctaaga actctctggg
      121 aaaaacatat ggtgatacag tgggagagaa gcaactcttg gcttcaatag gccaacccga
      181 actgatgaat ctctattcta gcttgtttaa aaatgaaaac attttagtat ctcagttatt
      241 acttaccaaa caagacttcc atactagaca gcattattta aatattgccc gtttattaac
      301 ggaattacta aagcatcaaa atattatccc tattgttaat gaaaatgatt cggtagccat
      361 tgaagaatta atgtttactg ataatgatga attagcagga cttattgcag ctcaaattaa
      421 tgctgattat ctaatactct taaccaatgt cgatggtgtg tttacatccc atcccaatga
      481 tcctgaatca aaactcatta atacaattga ccccaattca ggttggccta atgtctctgc
      541 tgtaaaatca aaattaggcc gaggtggtat ggtgtccaaa ctggatactg ccagaaaaat
      601 ggctggttta ggtatcaata cagtgatcgc aaatgtaaat gtagaatcca ttgttccaaa
      661 aattctaaat agggaagcag ttggaaccat aattcttgct agtaaaagaa aatccagtat
      721 aaaaagatgg attgctttta gtcatagaac ttccttacca tctatcgtaa ttaatgataa
      781 tttaattacc ttattacgaa atggtaatcg tgtaatgagc attttaccaa tagggataga
      841 agattttcgt ggagaatgca aaaaaggtga tttaattgat atccttaatt ccaaaaatga
      901 aaagatagga gttgggatcg ccagatatga tttcttaaga ttaaaggact atcttagact
      961 tcaaaataag cctgcattta tacattacga tcatttgcat attctatagg tatcaaaatg
     1021 aatattgata ttgctaacca attaagggcc gctaaaaaag caactactga tcttaactta
     1081 atacaaagtg atacccgaac aataatttt
//
LOCUS       DATNSN010000329         1089 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.333, whole genome shotgun sequence.
ACCESSION   DATNSN010000329 DATNSN010000000
VERSION     DATNSN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.333"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            304..>1089
                     /locus_tag="VAH76_003185"
     CDS             304..>1089
                     /locus_tag="VAH76_003185"
                     /inference="COORDINATES: protein motif:HMM:NF019313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387748.1"
                     /translation="MTDNLNANLNFLEDDFRRKPIAEKLIELLQSDIDISPIIIDGEW
                     GTGKTYFCKKTKSLIENDHQENISCAYIDAFSADHANEPLITIIAAISKLIKKGTNKR
                     KYLRAAKAIASYGIKATGKAGIAWILRTQAQHMDKEIEEAIQSASNDSIDYAIDNLIQ
                     QHEAIEENIITLKKVLSEIAQTKNLVIFIDELDRCRPDFAVSILEVIKHVFDVEGIKF
                     VLVTNLNQMKASINHCYGQAVNAHHYLDKFIKYSFKLPLDTKDV"
BASE COUNT          411 a          171 c          185 g          322 t
ORIGIN      
        1 taatattatt attgcgtacg agttcaattt actgagtaaa ataacatctc cacttttccc
       61 ttgtgcattt aaaatgttga aaaatctatt ttcgaaaaaa taaataggaa gaattataat
      121 caaagcgata atagtgacat aaaatgtgag tctaatgaga gaatatgagc tgtaaagatt
      181 ttttagatat gccaacatgc ttaccccgat gttttaccag atatcggcat tggtaataaa
      241 atctttaggt tacagcgata cattagcatc aggctcaacc tccaataatt tatggaaata
      301 ttgatgacag ataatttgaa tgcaaatctc aactttctcg aagacgattt tagaagaaaa
      361 cctatcgcag aaaaacttat cgaactactt caatcggata ttgatatttc cccaattatt
      421 attgatggtg aatggggtac gggaaaaaca tatttttgta aaaagaccaa atcattaatt
      481 gaaaatgatc accaagaaaa tatttcatgt gcttacatcg atgccttttc tgcagatcat
      541 gctaatgaac cattaattac aattatcgcg gcaatatcga aactcataaa aaagggaacc
      601 aataagagaa agtatttaag agcagctaaa gctattgcta gttatggcat aaaagctaca
      661 ggaaaagccg gaatagcatg gatattaaga actcaagccc agcatatgga caaagaaatc
      721 gaagaagcta tccaatctgc aagtaatgac agtattgatt atgcaattga taatttaatc
      781 cagcagcatg aagccataga ggaaaatatc attacattga aaaaagtatt atctgagatc
      841 gcacaaacta aaaacttagt tattttcatt gatgaattag accgctgccg accagatttt
      901 gctgtatcaa tattagaagt catcaagcat gtttttgatg tagaaggcat aaaatttgtt
      961 ttagtaacca atttgaatca aatgaaggcc tcaattaatc attgctatgg acaggcggtt
     1021 aacgcccacc actatttgga taagtttata aagtatagtt ttaaactccc gttggatacc
     1081 aaagatgta
//
LOCUS       DATNSN010000330         1083 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.334, whole genome shotgun sequence.
ACCESSION   DATNSN010000330 DATNSN010000000
VERSION     DATNSN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.334"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1083
                     /gene="amrB"
                     /locus_tag="VAH76_003186"
     CDS             <1..>1083
                     /gene="amrB"
                     /locus_tag="VAH76_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEO1387749.1"
                     /translation="LGPAHRLYFKGIAYDPVDKFATPLGEIQQDKELLTQIIDLPYVY
                     SLPEAHQNEHCLEVQLPFCQMIFSKFKILPLVVGETNPQDVARLIARVWGGDDTLLII
                     SSDLSHYLPYHIAQREDKKTCLSIDTLNGEEILHDAACGYFPLRGFLYFARQHHIYSR
                     LIDLRNSGDTAGDKERVVGYAAYHFYQKLNFSEHCADELKYIAKETIRLQTEENKALA
                     INYNDYNQLLQIRIPTFITLKKNGLLRGCMGSLIAKERLADNVIYNSIRAAIADPRFP
                     QIRPSELKELSLTISLIKPLSPLYFNSEEELKSQLQIGIDGLVLIYGSYQATFLPSVW
                     ESVKTKDEFVNHLKLKMGLTENFWSS"
BASE COUNT          362 a          208 c          190 g          323 t
ORIGIN      
        1 attaggccct gctcacagat tatatttcaa aggcatagcc tatgatcctg tcgataaatt
       61 cgctacacca ttaggagaaa ttcaacagga taaagagtta ctaactcaaa ttatagatct
      121 gccttatgtt tattcacttc ctgaagctca tcaaaatgaa cattgtctgg aagtacaatt
      181 gcctttctgc caaatgatat tctccaaatt taaaatactt cctctggttg ttggagaaac
      241 gaatccccag gatgtagccc gtctaatagc gcgagtatgg ggtggagatg atactctcct
      301 gattatcagc tctgatttaa gccattatct gccctatcac atagcgcaaa gagaagataa
      361 aaaaacttgc ctttctatag acactttaaa tggtgaagaa atactgcatg atgctgcttg
      421 tggttatttt ccactgcgcg gtttcctgta tttcgcacgc caacaccata tttatagccg
      481 tctgatcgat ttgcgcaact caggcgatac agctggagat aaagaaagag tagtgggata
      541 tgctgcctat cacttctacc aaaagcttaa tttttcagaa cattgtgcag atgaattgaa
      601 atacatcgcg aaagaaacaa tacgattaca aacagaggaa aataaagcat tggcaattaa
      661 ttacaatgat tataatcaat tattacagat acggatcccc acttttatta ctttaaaaaa
      721 aaatggtttg ctaagaggtt gcatgggaag ccttatagct aaagagcgat tagcagataa
      781 cgtcatttac aattccatac gggcagcaat tgctgaccca cgcttcccac aaataagacc
      841 atctgaactc aaggaattgt ctctaactat ctcattaata aaaccattat ctccactata
      901 ttttaacagt gaagaagaac tcaaatcaca attgcaaatt ggcatagatg gcttggtatt
      961 aatttatggt tcctatcaag ctactttttt accttctgta tgggagtctg ttaaaacaaa
     1021 agatgaattt gttaatcatt taaaattaaa aatggggctt acagaaaatt tctggtcttc
     1081 aga
//
LOCUS       DATNSN010000331         1072 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.335, whole genome shotgun sequence.
ACCESSION   DATNSN010000331 DATNSN010000000
VERSION     DATNSN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.335"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..294
                     /locus_tag="VAH76_003187"
     CDS             <1..294
                     /locus_tag="VAH76_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO1387750.1"
                     /translation="NSLEYIEETHKGEISIWLEQGWEKENFNYLHIKDTAKGLNHKEY
                     EKIFDFFYSGRKEGTGLGLAYCKLLMQAAGGDISCKGKKNEFAHFTLKFPKID"
     gene            complement(342..1037)
                     /locus_tag="VAH76_003188"
     CDS             complement(342..1037)
                     /locus_tag="VAH76_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEO1387751.1"
                     /translation="MPEETSTNLPKSLSHFLIQSDLDYKNSLSSIPLFDVKRTIFWDD
                     NLRKMFASIFYHLRGHFINFLWYVANFADNEASKQIIIQNIYEEIGIGNRFSHEKLYE
                     RFAKECGVDIHDEIVNETNYIPFAKEFNKAHLSWLAQHDPKDHLCAFAAYERLDNLDY
                     PFLVELAKSMKISQHGMTFFNVHTHVDHFDTTLQLIVPIWEESPQRLIESFHFIQTHQ
                     LGMWEQFSNVLFN"
BASE COUNT          390 a          173 c          185 g          324 t
ORIGIN      
        1 aatagtctgg aatatattga ggaaacccat aaaggcgaaa tctctatctg gctagaacaa
       61 ggatgggaga aagagaattt taattacctc catataaaag atacagctaa aggtttaaat
      121 cataaagagt atgaaaaaat atttgatttc ttctattctg gcagaaaaga aggaactggt
      181 ttaggcttgg cctattgtaa acttctgatg caagcagcag gtggtgatat ttcctgtaaa
      241 gggaaaaaaa atgaattcgc tcatttcact ttaaaattcc ccaaaattga ttagttccta
      301 tttaacataa gaaatcccaa gatttcttat gttaaaaaat atcaattaaa taagacattg
      361 gaaaactgtt cccacatccc taactgatga gtttgaataa aatgaaatga ttcgatcaga
      421 cgctgaggtg attcttccca tatgggcaca atcaattgta gcgtggtatc aaagtgatca
      481 acgtgggtat gtacattaaa gaaggtcatg ccatgttgag atattttcat cgatttggca
      541 agttcaacaa gaaaaggata atcaagatta tccaagcgct cataagccgc aaaagcacac
      601 aaatgatctt taggatcatg ttgcgccaac caggataaat gagctttatt aaattctttg
      661 gcaaaaggaa tatagttggt ttcattcact atttcatcat gaatatcaac accgcattct
      721 tttgcaaaac gttcataaag cttttcatga gaaaaacgat tgccaattcc aatttcttca
      781 taaatatttt gaataataat ttgttttgaa gcttcgttat cagcaaagtt ggcaacgtac
      841 cacaaaaaat ttataaaatg ccctcttaaa tgataaaaaa ttgatgcaaa catttttcta
      901 aggttgtcat cccagaaaat agttcgctta acgtcaaata aaggaatcga actcaatgag
      961 ttcttataat ccaaatcaga ttgaataaga aaatggctga gtgattttgg taaatttgtg
     1021 ctcgtctctt caggcatgat tcacacttca tatgaaataa gttaagtcca gc
//
LOCUS       DATNSN010000332         1060 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.336, whole genome shotgun sequence.
ACCESSION   DATNSN010000332 DATNSN010000000
VERSION     DATNSN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.336"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1016
                     /locus_tag="VAH76_003189"
     CDS             <1..1016
                     /locus_tag="VAH76_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387752.1"
                     /translation="FTEDVVERDSNIWIKIAQKKGILSAILSSSTATVTEAAETYFNN
                     PHYYFPTHLPLYLQIGLKIFIRKWCFNYRDRKFIRLPLKQLIAMDLLDLAPKQPWTIN
                     SGDSTLILVESQFSKDLLVKEGINRRRIKIIGSLKFDSMAPVLNDSTRYKQELYQKYN
                     LNLTKKLILCAFPPNLPNRLSYDFQNYEDMTKKWISTLQLSDYNVLYSVHPSGFPDTA
                     NAIRSLDQIVVEEDIANLIPLCDVYLASVSSTIKWARACNKLVLDYDCFHFNYRTYEK
                     DPAIIAVKSLNELKEWITRLNNQSVSKIHDQLNPSLHPNYWGIIDGRSLWRINNYIDC
                     LIK"
BASE COUNT          356 a          163 c          179 g          362 t
ORIGIN      
        1 tatttactga agatgtggtt gaaagagaca gtaatatatg gataaaaatc gcccaaaaaa
       61 aaggtatttt gtctgcaatt ctgagttcga gtactgctac tgtgactgag gctgcagaaa
      121 cttattttaa taacccgcat tattattttc ctacccattt gccattatac ctacaaatcg
      181 gattaaaaat ttttataaga aaatggtgct ttaattatcg tgataggaaa tttatacgcc
      241 ttcctctcaa acagttgatt gcgatggatt tattagattt agctccaaag caaccatgga
      301 caattaatag tggagactcc accttaatct tggttgaaag tcaattttcc aaagacttat
      361 tggtaaaaga aggtattaac aggaggcgaa ttaaaattat aggaagttta aagtttgact
      421 caatggctcc ggttttaaat gattcaactc gctataaaca agagctttat caaaaatata
      481 atttgaattt aactaaaaaa ctaattttgt gtgctttccc acccaacctt ccaaatcggt
      541 tatcatatga ttttcaaaac tatgaagata tgactaagaa atggatatct acgcttcaat
      601 tatctgatta caatgtttta tattctgtac acccaagtgg gtttcctgac acagcaaatg
      661 caattcgctc tctggatcag attgttgtgg aagaagacat agcaaatttg attcctcttt
      721 gtgatgttta tctggcttct gtttcaagta ctattaaatg ggcaagagct tgtaataagc
      781 ttgttttgga ttatgattgt tttcatttta attatcgcac ttatgaaaag gatcctgcaa
      841 ttattgctgt taaaagtctg aatgagctga aagaatggat tactcgcctc aataatcaat
      901 cagtatcaaa gatacatgat caattaaatc cttctctaca tccaaactat tggggaatta
      961 ttgatgggag gtctttatgg cgaattaata attatattga ttgcctgatt aaataacttt
     1021 gatactctat gaatgtttaa aaaaattgta actaattaag
//
LOCUS       DATNSN010000333         1052 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.337, whole genome shotgun sequence.
ACCESSION   DATNSN010000333 DATNSN010000000
VERSION     DATNSN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.337"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            444..977
                     /locus_tag="VAH76_003190"
     CDS             444..977
                     /locus_tag="VAH76_003190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387753.1"
                     /translation="MTSLIEFKKQAKILRDFLGQKNHDITHSSCLEAIAIMNGFKNWN
                     TMSGIFAKNGNALEKRSKTWICDTCGGLIQKPEEGWVEWIVSVPKNEAYGLRLAHRAG
                     YGSNGSSCQYNEELVYKQYGGIINDGELSWYLGDGGLMLLLTLLEEQQIPQDQVLEMI
                     KRLHIPEYEHARRHFNE"
BASE COUNT          365 a          163 c          184 g          340 t
ORIGIN      
        1 gaaatgatat cattaattaa acagataaaa tttccaaaaa tattaaacag gaatctgaat
       61 tatttggatt tgttgaattt aattttatcg ccagatacaa atgtaaacat cccataattt
      121 gtggcaacct aaattagagt tcattcataa taccttctaa ttcccccatt cactgatgca
      181 atttactttg caatattgtt gttccatctc atctttcatt tggttataaa gtacagatta
      241 atttctccgt ccaaaatata agttgacata aattcataat tagtagcaaa attcaattcg
      301 gtttgaaact gacctatccg cctacaccct ttaaagggac ggtaaatagt ttctacgcta
      361 tcctaaatca taattagatc tttaatggtt agcttccaaa atgtaggcat tggcaacagc
      421 aaataactat tgaggaatta aatatgactt ctcttattga atttaaaaaa caagcaaaaa
      481 tcttacgcga ttttcttggc caaaaaaatc atgatattac acattcttct tgtttagagg
      541 ctattgcaat aatgaatggc tttaagaatt ggaatactat gtcagggatt tttgcaaaaa
      601 atggtaatgc attagaaaaa agatcgaaaa cttggatttg tgacacgtgt ggtggattga
      661 ttcagaaacc agaagaggga tgggtagaat ggattgtatc tgtacctaaa aacgaggcat
      721 acggtcttcg cttggcgcat cgtgctggct atggctcaaa tggaagttct tgtcaatata
      781 atgaggaatt agtgtataaa caatatggtg gtataataaa tgatggagag ctgtcttggt
      841 atctcggaga tggcgggcta atgttattac tcaccttgct tgaagaacaa cagataccac
      901 aagaccaagt actggaaatg atcaaacgac ttcatattcc tgagtatgaa catgctcgtc
      961 gtcactttaa tgagtaggat ccttaaagta taaattttta aatttttaag aaaaatcgag
     1021 tttttgctta ataaatttga gccttacaat aa
//
LOCUS       DATNSN010000334         1045 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.338, whole genome shotgun sequence.
ACCESSION   DATNSN010000334 DATNSN010000000
VERSION     DATNSN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.338"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>1045)
                     /locus_tag="VAH76_003191"
     CDS             complement(<1..>1045)
                     /locus_tag="VAH76_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEO1387754.1"
                     /translation="FFIGPGETNDVLVKIKKDQPYIIYAESFDKVGTALGALVTSSNQ
                     HIPFEKVKPFPDPKPATRTKMKNEKARGLMPMKHMGMQSTLHHQPLINTIAAAHTPQK
                     INKQHHQSKKEAYVTVGTKYQDLKAQKVTNDPSKPIYKTVEMKLFGFMDRYIWFINGV
                     PAYNAKPINLLPGKRYRLVFTNNSMMHHPLHLHGHWMILRNGHGKYDPLLHTIDVPPG
                     SMVTADLDTDASGQWFFHCHMLNHMISGLARTFQYNDIIKVAENEKKPAHVIKETGFY
                     NRPIVRVDELRPIPLSIVKNAKSHPQQMWAASFLEFGGDFNKNRQTLTYLGLYGYDFH
                     KLQLFVNDAEMSKG"
BASE COUNT          287 a          197 c          220 g          341 t
ORIGIN      
        1 cctttgctca tttcagcatc atttacaaat aattgtaact tatgaaagtc ataaccatat
       61 aatccaagat aagtcagggt ttgacggttt ttattaaaat ccccgccaaa ttccaaaaac
      121 gaagcagccc acatttgctg aggatgagat tttgcatttt tgacaatgga aagaggtata
      181 ggtcttagtt catcaactct aacaatgggg cgattataga agccggtttc cttaatcaca
      241 tgagctggct ttttctcatt ttcggccact ttaatgatat cattgtattg aaacgttctg
      301 gcaagccctg aaatcatatg attgagcata tgacaatgaa aaaaccactg accacttgca
      361 tcggtgtcta aatcggctgt gaccatactt cctggcggca catcaatggt atgtagcaga
      421 gggtcatatt ttccatggcc atttcgcaaa atcatccagt gcccatgcag atgcaaagga
      481 tgatgcatca tagagttgtt agtaaagacc aagcgatagc gtttaccagg caataaatta
      541 atcggttttg cattataagc aggcacgcca ttaataaacc aaatgtatcg gtccatgaag
      601 ccaaataact tcatttcaac ggttttataa atgggcttac ttgggtcgtt agttaccttt
      661 tgggctttta agtcctggta ttttgtgcca accgttacat aggcttcttt tttactttgg
      721 tgatgttgct tatttatctt ttgtggtgtg tgtgctgcgg caatggtatt gatgagtggc
      781 tgatgatgca gcgtactttg catgcccata tgcttcatag gcataaggcc ccgggctttt
      841 tcgtttttca ttttcgtacg ggttgcaggt tttgggtcgg gaaaaggttt cactttctca
      901 aaagggatgt gctgattgga agatgtgact aatgcaccta aagctgttcc aactttatca
      961 aatgattctg catagatgat atagggttgg tcttttttaa ttttgaccaa cacatcattg
     1021 gtttcaccag gtccaataaa aaagt
//
LOCUS       DATNSN010000335         1044 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.339, whole genome shotgun sequence.
ACCESSION   DATNSN010000335 DATNSN010000000
VERSION     DATNSN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.339"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            480..689
                     /locus_tag="VAH76_003192"
     CDS             480..689
                     /locus_tag="VAH76_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387755.1"
                     /translation="MQINKPEKLLLRYRNMSSELTPARSIVALTHDEDNKVLQCEIQY
                     LVTKKLKFKKVIDELKTTIPYPVEG"
     gene            complement(890..>1044)
                     /locus_tag="VAH76_003193"
     CDS             complement(890..>1044)
                     /locus_tag="VAH76_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387756.1"
                     /translation="FIKYKTPATSYGTFWFQKVQLSESRMDEVLAQRSEQRENRRHTG
                     DSPNPT"
BASE COUNT          340 a          167 c          202 g          335 t
ORIGIN      
        1 gtgctcaata tgatcaactg ttattttttg ataggtgaag tcttattttt tattagcata
       61 agcttaataa atccctaata atttttaact agattgacta ttataggcgc aattaagtgg
      121 tgagaacagg ctatcttacg tgaataaggt agatttatta agtaaggtta atgacgccca
      181 gtttggattg tgctcacttt agagaaaaaa agagttgaat tttatcaata ggtcagggat
      241 gcaacagatg caattcccct tgatcaacgc ataacccatg ctgcacccat aattgaagcg
      301 aatagcacct tgctccgcaa gttatctcgc ttaacaagac caatagattt tgcaattgat
      361 aaaatagctg gggagggtga gagtgaaact cttctcagta aatgcgggaa gagtttacta
      421 ggctaattgg aaaaatatta gtaatttctc aggatgattt aagcaaggag ggatgattga
      481 tgcaaatcaa caaacctgag aaattattat tacgttatcg aaatatgtcg agtgaactga
      541 ctcctgcaag atcaatcgtt gcgttaaccc atgatgaaga caacaaagtt ctgcagtgtg
      601 aaatacaata tctggttact aaaaaactga aattcaaaaa ggtcatagat gaattaaaaa
      661 cgacgattcc ttatccagtt gaggggtgag caaagacagt taaatcaaat taaaaccagc
      721 ataacaggag gcaaattgtt aattaactca tgttacgcac aatcaatgtt gagcagtcat
      781 atttttaaat tagataccaa atcttctgct gcgtttgcca tttaatttca ttttgttctt
      841 tttgaaaaaa ggcatgcttg ttcatgcctt tttcaataat cttgtttgat tatgttgggt
      901 ttggactatc gcctgtatgt ctacgatttt ctcgttgttc acttcgctgg gcaagcactt
      961 catccattct gctttcggat agttgtactt tttgaaacca aaaagtgccg tatgaagtag
     1021 caggagtttt atatttaata aact
//
LOCUS       DATNSN010000336         1041 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.340, whole genome shotgun sequence.
ACCESSION   DATNSN010000336 DATNSN010000000
VERSION     DATNSN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.340"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(249..>1041)
                     /locus_tag="VAH76_003194"
     CDS             complement(249..>1041)
                     /locus_tag="VAH76_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEO1387757.1"
                     /translation="NFIIKNSRIIGVGPNSNSPHSFATGIYSEGNLCSIESNTISLGY
                     ATGNGENVGIALYGGIDCRIKNNHIIFARWPMWGRNFGIWSKSSRSGHPVIENNFIRG
                     AHYALGPFGLFSGNIAVNLSCAFFVQRKNKANQFFDLGKNVAVMQSSVNEGMGGNSIC
                     PDNRVYAAYRFIVNPGAFSAYSAAMAWGEGGEETLIETYAWFLVASYYKHELALNIVN
                     NPFANGYSENVVSKAKIIADKIFIGNILLKTLKMINCIFQSISDV"
BASE COUNT          370 a          194 c          135 g          342 t
ORIGIN      
        1 ttattatcaa gtgaatgttc acataaatac tggctacaaa gtaaaattaa attaaaaaac
       61 ccgaataaat ccttagataa aatattaaat atcttttcaa gtcttcatta tcagataact
      121 tagttattga aaataataat ttaataacta agaaatagat atctatgttt tcttgttgat
      181 ttactataat tgcaagtgcc tatgttttaa ttatccataa tcgttcaagt gcaacattat
      241 cgacattttc aaacatctga gattgattga aaaatacaat ttatcatttt tagagtcttc
      301 agcaggatgt taccaataaa aatcttatca gctattattt ttgcttttga gactacattt
      361 tctgaataac cattagcgaa tggattgttt actatgttta aagcaagctc atgtttgtag
      421 taagaagcaa ctaaaaacca tgcataagtt tcaattaatg tttcttctcc cccttctccc
      481 caagccattg cggcagaata agctgaaaat gctccaggat ttactataaa tcgatatgca
      541 gcataaactc tgttatcagg acaaatactg ttacccccca ttccttcgtt tacagaagat
      601 tgcataactg caacattctt acccaaatca aaaaattgat ttgctttatt ttttctttga
      661 acaaaaaaag cacatgacaa gttaacagct atatttccac taaatagtcc gaagggacca
      721 agagcataat gtgcacccct gataaagtta ttttcaataa caggatgacc acttctggac
      781 gacttgctcc aaattccaaa atttcgtccc cacattggcc aacgcgcaaa aataatatga
      841 ttattcttta tacgacaatc tattccacca tataaagcaa ttccaacatt ttctccatta
      901 cctgtagcat atcctagact tatggtatta gactcaattg aacagagatt accttcagaa
      961 taaatccctg tagcaaaaga atgtggtgaa ttagagttag ggcccacacc aattattcgt
     1021 gaattcttta taataaaatt a
//
LOCUS       DATNSN010000337         1024 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.341, whole genome shotgun sequence.
ACCESSION   DATNSN010000337 DATNSN010000000
VERSION     DATNSN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.341"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..396
                     /gene="cas1f"
                     /locus_tag="VAH76_003195"
     CDS             <1..396
                     /gene="cas1f"
                     /locus_tag="VAH76_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO1387758.1"
                     /translation="YAANRTQYGKFIREREAIDKANIFLNHGNYLAYGLAATTLWVLG
                     IPHGFAVMHGKTRRGALVFDVADLIKDALILPWAFICAKENSSEQEFRQQCLQAFIDH
                     KALDYMFEQVKAMALNTDWESENSEENLL"
     gene            393..>1024
                     /locus_tag="VAH76_003196"
     CDS             393..>1024
                     /locus_tag="VAH76_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HEO1387759.1"
                     /translation="MMVIFVSQCEKKALARTRRVLDAFADRIGDNTWQTVITQEGLLA
                     VKKLLRKTASKNTAVSCHWIRSRSRTELVWVVGNKSKFDREGRVAVNSTSKNQVYRDD
                     LLDWKLLPVIQSLACLAALLHDWGKANARFQQKLAKNFKGIPADRLRHEWVSCLLLKA
                     LILNTDNPYSDSDWLGTLKDGIINEDAIKTALRMSCEKPLADLPNIAKLIA"
BASE COUNT          310 a          196 c          245 g          273 t
ORIGIN      
        1 tacgcagcaa accgaactca gtatggcaaa tttattcgtg agcgggaagc tatagataag
       61 gccaatatat ttttaaatca tggcaattac cttgcctatg gtttagcggc aacaacactg
      121 tgggttcttg ggattcctca cggctttgca gttatgcacg gcaagacacg tcgcggtgca
      181 ttggtatttg atgtagcgga tttaatcaag gacgccctta ttctgccttg ggcatttatc
      241 tgcgccaagg aaaatagtag cgaacaagag tttcgtcagc aatgtttaca ggcattcatc
      301 gatcataaag cgctggacta tatgtttgaa caagtaaaag caatggcctt aaatacagat
      361 tgggaaagcg aaaatagcga ggaaaacttg ctatgatggt tatttttgtt tcccagtgcg
      421 agaaaaaagc gcttgctaga acccgtcgtg tcttagatgc ttttgccgat cgcatcggcg
      481 acaacacgtg gcaaactgta atcacccagg aaggattgct cgccgttaag aaactgctgc
      541 gcaaaaccgc cagtaaaaat acagcagtaa gttgtcactg gattagatca agatcgagaa
      601 cggagttagt ttgggtggtg gggaacaaga gtaagttcga tcgcgaaggt agagtagcgg
      661 tcaatagtac atctaaaaat caggtttatc gagatgactt gttagattgg aagctgcttc
      721 ctgtcattca atcgttggct tgtttggcag ctttactgca tgactggggt aaggcgaacg
      781 cccgcttcca gcaaaaactt gccaaaaatt ttaagggtat acctgcagac cgattacgtc
      841 atgagtgggt atcatgttta ttactcaaag cacttattct caatactgat aatccatatt
      901 cggacagtga ttggttggga acactaaaag acggaataat caatgaagat gccattaaaa
      961 cagcactacg aatgagttgt gaaaaacctt tggccgattt accgaatatc gcaaaattaa
     1021 ttgc
//
LOCUS       DATNSN010000338         1024 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.342, whole genome shotgun sequence.
ACCESSION   DATNSN010000338 DATNSN010000000
VERSION     DATNSN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.342"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..396
                     /gene="cas1f"
                     /locus_tag="VAH76_003197"
     CDS             <1..396
                     /gene="cas1f"
                     /locus_tag="VAH76_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO1387760.1"
                     /translation="YAANRTQYGKFIREREAIDKANIFLNHGNYLAYGLAATTLWVLG
                     IPHGFAVMHGKTRRGALVFDVADLIKDALILPWAFICAKENSSEQEFRQQCLQAFIDH
                     KALDYMFEQVKSMALNTDWESENSEENLL"
     gene            393..>1024
                     /locus_tag="VAH76_003198"
     CDS             393..>1024
                     /locus_tag="VAH76_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HEO1387761.1"
                     /translation="MMVIFVSQCEKKALARTRRVLDAFADRIGDNAWQTVITQEGLLA
                     VKKLLRKTASKNTAVSCHWIRSRSRTELVWVVGNKSKFDREGRVAVNSTSKNQVYRDD
                     LLDWKLLPVIQSLACLAALLHDWGKANARFQQKLAKNFKGIPADRLRHEWVSCLLLKA
                     LILNTDNPYSDSDWLGTLKDGIINEDAIKTALRMSCEKPLADLPNIAKLIA"
BASE COUNT          309 a          195 c          244 g          276 t
ORIGIN      
        1 tacgcagcaa accgaactca gtatggcaaa tttattcgtg agcgggaagc tatagataag
       61 gccaatatat ttttaaatca tggcaattac cttgcctatg gtttagctgc aacaacactg
      121 tgggttcttg ggattcctca cggctttgct gttatgcacg gcaagacacg tcgcggtgca
      181 ttggtatttg atgtagcgga tttaatcaag gacgccctta ttctgccttg ggcatttatc
      241 tgcgccaagg aaaatagtag cgaacaagag tttcgtcagc aatgtttaca ggcattcatc
      301 gatcataaag cgctggacta tatgtttgaa caagtaaaat caatggcctt aaatacagat
      361 tgggaaagcg aaaatagcga ggaaaacttg ctatgatggt tatttttgtt tcccagtgcg
      421 agaaaaaagc gcttgctaga acccgtcgtg tcttagatgc ttttgccgat cgcatcggcg
      481 acaacgcgtg gcaaactgta atcacccagg aaggattgct cgccgttaag aaactgctgc
      541 gcaaaaccgc cagtaaaaat acagcagtaa gttgtcactg gattagatca agatcgagaa
      601 cggagctagt ttgggtggtg gggaacaaga gtaagttcga tcgcgaaggt agagtagcgg
      661 tcaatagtac atctaaaaat caggtttatc gagatgactt gttagattgg aagctgcttc
      721 ctgtcattca atcgttggct tgtttggcag ctttactgca tgactggggt aaggcgaacg
      781 cccgcttcca gcaaaaactt gccaaaaatt ttaagggtat acctgcagac cgattacgtc
      841 atgagtgggt atcatgttta ttacttaaag cacttattct caatactgat aatccatatt
      901 cggacagtga ttggttggga acactaaaag acggaataat caatgaagat gccattaaaa
      961 cagcactaag aatgagttgt gaaaaacctt tggccgattt accgaatatc gcaaaattaa
     1021 ttgc
//
LOCUS       DATNSN010000339         1023 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.343, whole genome shotgun sequence.
ACCESSION   DATNSN010000339 DATNSN010000000
VERSION     DATNSN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.343"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            14..286
                     /locus_tag="VAH76_003199"
     CDS             14..286
                     /locus_tag="VAH76_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO1387762.1"
                     /translation="MRITPESISTCVQYPHVFGALSEFERNLIKERTRAGLEAARGHQ
                     GGRPEKLSDEQKELVKTLYSNKRHSIQSTCKMVGVGKTTLYKYINQ"
     gene            473..>1023
                     /gene="cas1f"
                     /locus_tag="VAH76_003200"
     CDS             473..>1023
                     /gene="cas1f"
                     /locus_tag="VAH76_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO1387763.1"
                     /translation="MEILTDLKAILHSKRANIYYLEKCRIMQKDGRVLYLTEAKNENQ
                     YWNIPIANTTCLLLGTGTSITQAAMRMLAQAGVLVGFCGGGGTPLLMANEVEWLTPQS
                     EYRPTEYMQGWMKFWFDEQKRLYAAKQFQLSRIEYLRVHWKKSRDLAAEGFNSDDLQP
                     ELLSSELKIKKAKEVGKLMQNEA"
BASE COUNT          351 a          182 c          216 g          274 t
ORIGIN      
        1 gactgacaaa aatatgagaa ttactccgga atccataagc acttgcgttc aataccctca
       61 tgtttttggg gcgctttctg aatttgagcg aaatttaatt aaggaacgta cccgtgctgg
      121 tcttgaagca gcaagagggc accaaggtgg tcggcctgag aagcttagcg atgagcaaaa
      181 agagttggta aaaacacttt attccaataa aaggcattca attcaatcta cctgcaagat
      241 ggttggcgta ggaaagacta cgctttataa atatattaat cagtaattgt tgatgcgatt
      301 atacttttta taggtgaata tcaaattgaa aagcgaattg atatccttgt taaggaaggc
      361 aaataatagc caaatttatt tggtgggccc cagatgcgct ccttataaaa ttattgtgct
      421 atttattatt aataacaata ttatgcttaa tttttgaagc cacaaaatat caatggaaat
      481 actaacagac ttaaaagcta ttctacattc aaaacgtgcc aatatttatt acctggaaaa
      541 atgccgcatt atgcaaaagg atgggcgcgt cttgtacctc accgaggcaa aaaacgaaaa
      601 ccaatactgg aacataccta ttgccaacac cacttgtctt ttacttggaa cgggaacatc
      661 tatcacacag gcagcaatga ggatgctggc tcaggctggt gtattggttg gcttctgtgg
      721 cggaggcggt acacctttac tgatggccaa cgaagttgaa tggctcacac ctcaaagcga
      781 atatagaccc acagaatata tgcaggggtg gatgaaattc tggtttgatg agcaaaaacg
      841 tttatatgca gcaaaacaat ttcaactttc acgcatagaa tacctgagag ttcactggaa
      901 aaaaagccga gatttagctg cagaaggttt taattctgac gacttgcagc cagaattatt
      961 aagcagtgaa ctaaaaatta agaaagcaaa agaagttggc aaattgatgc aaaatgaagc
     1021 gga
//
LOCUS       DATNSN010000340         1023 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.344, whole genome shotgun sequence.
ACCESSION   DATNSN010000340 DATNSN010000000
VERSION     DATNSN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.344"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(101..862)
                     /locus_tag="VAH76_003201"
     CDS             complement(101..862)
                     /locus_tag="VAH76_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387764.1"
                     /translation="MKLHSISTNKMRLIISMWLIYLFAVLFLVASLFTMLILMMEYTL
                     VCKNKQYHLAKECVLHSKIYNLYHSSTELGDLKAAVVKSSRSSKGNTLYYVDLLTSQG
                     PVNLTGSSSSGRDDKEIAANSINNYISNSLETTFKVSYPASWWMYGLSVFFLLVGALL
                     LTVRGAITDFDRILKTIVIKRKGLFKTEETKLSFSDVDKIIVEESLGSRGTKTYRLAI
                     ALKDKPPIPLVTTYDSSFAKKEKIANQLNQFIHES"
BASE COUNT          326 a          189 c          168 g          340 t
ORIGIN      
        1 gtttagataa actaaatgac cgaattaaaa atttttttta acaaagagat tggggttttt
       61 cgtaaatcct gtaagcctgt ttataatttt tttggaacaa tcagctctca tggataaatt
      121 gatttaattg gtttgcaatt ttttcttttt tagcaaaaga agaatcatac gttgttacta
      181 aaggaatagg tggcttatcc tttagagcga tggcaagtct ataagttttc gtacctctgc
      241 tcccaagcga ctcttcaact attattttat ctacatcaga aaacgataat ttggtttcct
      301 ctgttttaaa caatcccttt cttttgatta caattgtttt taatattctg tcaaaatcag
      361 taattgcccc tctaacggta agcaacagag cacccaccaa tagaaaaaaa acggataagc
      421 catacatcca ccacgaggcg ggataactca ctttaaaagt cgtttccaaa ctattgctta
      481 tgtagttatt gatgctgttg gcagcaattt ctttatcgtc acgcccgctg gaactgctcc
      541 ccgtcagatt gactggacct tgagacgtta acaaatccac ataataaagc gtatttcctt
      601 tacttgagcg actgctttta actacggctg cttttaaatc gcctaactcg gtgctggagt
      661 gatataaatt ataaattttg ctgtgtaaaa cacattcctt agccaaatgg tattgtttgt
      721 ttttgcaaac gagggtgtat tccatcatta aaataagcat tgtaaaaaga ctcgcgacaa
      781 gaaacaaaac cgcgaacaga tatataagcc acatgctgat aatgaggcgc atcttgtttg
      841 tgctgataga gtgtagtttc atttcatatt attgctgata acttatttta aatatagcaa
      901 caactttcga ttatatgtaa tggttaatac cattagtcaa tgtaaaagcc attccacaat
      961 gaccaaaaca aaaccacaag ggggctattt ttctactaaa tgctattttt tgccacaaat
     1021 aaa
//
LOCUS       DATNSN010000341         1014 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.345, whole genome shotgun sequence.
ACCESSION   DATNSN010000341 DATNSN010000000
VERSION     DATNSN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.345"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..587
                     /locus_tag="VAH76_003202"
     CDS             <1..587
                     /locus_tag="VAH76_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HEO1387765.1"
                     /translation="IGQQTGELHKTIIGSDQFITPRDLVTVIHDDFHPENTFYNLETN
                     IFSLIDNESMGDSITEPLPIIRELYGFYEVPIIRWPSEKITVDLLKDADPHDIASIYY
                     DFVLGMASVYQDPQDAKRVLIEAIIDLNNMAIAFLKDRYSEPWMLNPSDKHEAHIVWG
                     KLAKVDTNPQLAELYITKLTIINNVLQSLSGFKR"
BASE COUNT          330 a          174 c          175 g          335 t
ORIGIN      
        1 gaatagggca acagacgggt gaattacata aaacaattat cgggagtgat caatttatca
       61 ctccaagaga tttagtgacc gttattcatg atgattttca tcctgaaaat acgttttata
      121 atcttgaaac aaatatcttc tctttaattg ataatgaaag catgggtgat agtattacag
      181 agcccttgcc cattatcaga gaattatatg ggttttatga agttcctatt attcgctggc
      241 cctcagaaaa aataactgtt gatctcttaa aggatgctga tcctcatgat atagccagta
      301 tctattacga ctttgttctc ggtatggcct cggtgtatca agacccccaa gatgcaaaac
      361 gagtgttaat tgaagctatt attgatttaa ataatatggc tattgccttt ctgaaggata
      421 gatattctga gccatggatg cttaacccaa gcgataaaca tgaagcacat attgtgtggg
      481 gcaagttagc aaaagtcgac actaatccgc agctagcaga actctatatc actaaattaa
      541 ccattattaa taatgtattg caatcacttt caggctttaa acgctagcag agggtgagta
      601 ctctggttat tccgcaccct cggattttag ctgcttcagc agtctttcat tatttgttta
      661 aatgtaataa tggggctgaa ttttaattac tctatatact gatgtagtgt gagcttattt
      721 tagattattt acttttgatt ttcagaagct gcataaaccc caatacatca tctacttatc
      781 tatttattta aagtgtatcg ctgctttata tctccttgct cattgataaa agacaccttg
      841 gtaaaggatg aatcatctga ctaacttcta aattccacaa gaaatttgac aaactataaa
      901 cagacttatc cacagaaaat gtggataact taaataaaat tacaggtatt aaaaaatgat
      961 atcaaccaag cccaataagg attgttatag tttactgatt aatcggttgt gatt
//
LOCUS       DATNSN010000342         1014 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.346, whole genome shotgun sequence.
ACCESSION   DATNSN010000342 DATNSN010000000
VERSION     DATNSN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.346"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(102..596)
                     /locus_tag="VAH76_003203"
     CDS             complement(102..596)
                     /locus_tag="VAH76_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEO1387766.1"
                     /translation="MSHFSILVEEIAAYLKKAKWQLVTAESCTGGLIAACLTEIPGSS
                     NWFERGFVTYSNLAKEEMLAVAHELIQTHGAVSEEVALAMAAGALQHSMGNIAVSVTG
                     IAGPEGGSIEKPVGTVCFAWAVHHQFQQTSRQQFTGSRQEIRLSACQYALNGVLSTLK
                     KVCG"
     gene            complement(674..871)
                     /locus_tag="VAH76_003204"
     CDS             complement(674..871)
                     /locus_tag="VAH76_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387767.1"
                     /translation="MNRLAMIAAASCLALFLTGCGEGSKPADTKTDQQQQQQQPTDQQ
                     QQGGENTGNNQAQPSTEGQGQ"
BASE COUNT          321 a          209 c          164 g          320 t
ORIGIN      
        1 tagctgaatg gtaaaaacaa gaatttgaga ctaaataata gaattgaaaa aatatagact
       61 attgatgttt tattaaacta tgtttttaaa aaaccattcg gttagccaca gactttttta
      121 agagtagaaa gaactccatt taaagcatat tgacaggcag acaaacgtat ttcttgccga
      181 cttccagtga attgttgtct tgatgtttgt tgaaattgat ggtgaactgc ccaggcaaag
      241 catacagtac ctactggttt ttcaatactg cctccttctg gacctgcgat accagtaact
      301 gatacagcga tattacccat actatgttgt aacgcaccag cagccatagc caaagccact
      361 tcttcactga ctgccccatg agtttgtatt agctcgtgag caacagcaag catttcctct
      421 ttagctaaat tactataagt aacaaaacca cgctcaaacc aatttgagct tccaggtatt
      481 tccgtaaggc aagcagcaat taaaccacca gtacaagatt ccgcagtcac taattgccat
      541 ttggcctttt ttaaataggc agctatttct tcaaccagta tcgagaaatg actcatacct
      601 ttcgcctata aaaaacccca taaaacatct atggggtttt cttgataatt tataaataac
      661 actgaaatta atcttactgg ccttggcctt cagtagaagg ttgagcttgg ttatttccag
      721 tattttcacc accttgttgt tgctgatcag tgggttgttg ctgctgctgt tgttgatcag
      781 tctttgtatc tgcaggcttc gagccctcac cgcatccagt taaaaataat gctagacaac
      841 tagcagcagc aatcatagct aaacgattca taatcactct ccctatttat tttttgtaat
      901 atcacgttaa ttctagcaaa tattctcatt aagtgaagaa cttacctcct gaacttaaaa
      961 aataaaattt atattttatt gcagtatgtt aaattaaatg acataaacca tgcc
//
LOCUS       DATNSN010000343         1011 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.347, whole genome shotgun sequence.
ACCESSION   DATNSN010000343 DATNSN010000000
VERSION     DATNSN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.347"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1011
                     /locus_tag="VAH76_003205"
     CDS             <1..>1011
                     /locus_tag="VAH76_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEO1387768.1"
                     /translation="ATAMLAVPGAITVRAYSYFGDSYIYIIFKDGTDLYWARSRVLEY
                     LNQVAGQLPPGAKPELGPDATGVGWVYQYALVDESGTHNLSQLTTLQNWLLKFELQTV
                     PGVSEVATVGGMVKQYQVVLEPNRIRAYGISLAKVKHAIMNANSEIGGSVIEMGEAEY
                     MVRTDGYIQSIQALKNIPLGVNEQGTPILLSNVARITLGPQMRRDVGELNGKGEAVGG
                     VIVMRMGDNALATIQGVKDKLASIQKSLPKGVKIVSVYDRSGLIYRAIDNLSITLLIE
                     FIVVASVCMLFLFHFRSSLVVILSLPIGVLAAFIVMYLQGINANIMSLSGIAIAIGVM
                     VDA"
BASE COUNT          260 a          203 c          246 g          302 t
ORIGIN      
        1 gcaacagcca tgttggctgt accaggtgcg attacggtca gggcctattc gtattttggt
       61 gattcctata tctatattat ttttaaagat ggtacagatt tatattgggc ccggtctcgg
      121 gttttggaat acttaaatca agttgcaggc caattacccc caggcgcgaa gccagagttg
      181 ggccctgatg caaccggcgt tggctgggta tatcaatatg ccttggtcga tgagtcgggt
      241 actcataacc tatcgcaact gaccaccctg caaaactggc tgttaaaatt tgaattacaa
      301 accgtacctg gtgtttctga agttgccacg gttggtggca tggtcaagca atatcaagtt
      361 gttttggaac ctaatagaat tcgtgcttat ggtatctctt tggccaaagt taaacatgcc
      421 atcatgaatg cgaacagtga gattggtggt tccgtcattg aaatgggcga agccgagtat
      481 atggttcgta ccgatggtta tattcaaagt attcaagctc ttaaaaacat ccccttgggt
      541 gtgaatgaac agggaactcc catcttgctt tcaaatgttg ctcgtatcac acttggacca
      601 caaatgcgac gcgacgttgg tgaacttaac ggtaagggag aagcagtcgg aggcgtcatc
      661 gtcatgcgca tgggcgataa tgctttagca accattcaag gcgtcaaaga taaattggcc
      721 agcatccaga aaagcttacc taaaggcgtc aaaattgtat cggtgtatga ccgctcaggg
      781 cttatatatc gtgcaataga caacctaagt atcaccttgc tgattgagtt tattgtcgta
      841 gcaagcgttt gcatgctgtt tttatttcac tttcgctcat cgctcgtggt cattttaagt
      901 ttaccaatcg gtgtgttagc ggcatttatt gtgatgtatc ttcaaggtat caatgccaat
      961 attatgtcgt taagcggtat tgcgattgcc ataggtgtga tggtcgatgc a
//
LOCUS       DATNSN010000344          997 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.348, whole genome shotgun sequence.
ACCESSION   DATNSN010000344 DATNSN010000000
VERSION     DATNSN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.348"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..977
                     /locus_tag="VAH76_003206"
     rRNA            <1..977
                     /locus_tag="VAH76_003206"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          212 c          312 g          215 t
ORIGIN      
        1 gcgtaaaggg tgcgtaggtg gttgattaag ttatctgtga aattcctggg cttaacctgg
       61 gacggtcaga taatactggt tgactcgagt atgggagagg gtagtggaat ttccggtgta
      121 gcggtgaaat gcgtagagat cggaaggaac accagtggcg aaggcggcta cctggcctaa
      181 tactgacact gaggcacgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca
      241 cgctgtaaac gatgtcaact agctgttggt tatatgaaaa taattagtgg cgcagcaaac
      301 gcgataagtt gaccgcctgg ggagtacggt cgcaagatta aaactcaaag gaattgacgg
      361 gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag aaccttacct
      421 acccttgaca tacagtgaat tttgcagaga tgcattagtg ccttcgggaa cactgataca
      481 ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgtaacgag
      541 cgcaaccctt atccttagtt gccagcatgt gatggtgggg actctaagga gactgccggt
      601 gacaaaccgg aggaaggcgg ggatgacgtc aagtcatcat ggcccttacg ggtagggcta
      661 cacacgtgct acaatggccg atacagaggg cggcgaaggg gcgacctgga gcaaatcctt
      721 aaaagtcggt cgtagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct
      781 agtaatcgcg aatcagcatg tcgcggtgaa tacgttcccg ggccttgtac acaccgcccg
      841 tcacaccatg ggagtgggtt gcaccagaag tagatagtct aaccttcggg gggacgttta
      901 ccacggtgtg gttcatgact ggggtgaagt cgtaacaagg tagccgtagg ggaacctgcg
      961 gctggatcac ctccttacat agaaaggcac agaagga
//
LOCUS       DATNSN010000345          987 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.349, whole genome shotgun sequence.
ACCESSION   DATNSN010000345 DATNSN010000000
VERSION     DATNSN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.349"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            44..>987
                     /locus_tag="VAH76_003207"
     CDS             44..>987
                     /locus_tag="VAH76_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HEO1387769.1"
                     /translation="MISSKHYKESMTLLVEGSLRAIPFNIILATLLAIDLVYNNVPAL
                     IIGVWIFAIVFISLVRSLFCWKIIKRGVEQNKIQSTLLKFFLLTFVMGSIWGACYFIT
                     LPYLNDLHEFIIILVFGGMCAGAIASLSVYLPAYYAYVFPMLIPVIIYNYALLDLDRT
                     ILATMFLIFIAMLIISARINNRLLNQNFRLFNEKELLISELKVMTITDSLTGLYNRRY
                     FDTLFPQEFNRARRNQYFLSLVSIDADNFKLINDNLGHPFGDKVLISIADLLKKIFRR
                     SNDILFRLGGDEFAIIIANQPIKDTISICQSIKNPFKT"
BASE COUNT          301 a          147 c          176 g          363 t
ORIGIN      
        1 ataattttaa agagtggata ttaatttatg gacattggat aaaatgattt catccaagca
       61 ttacaaggaa agtatgacct tgctggttga gggatctcta agagcaattc cgttcaatat
      121 cattttggct acattgcttg ccattgacct tgtttataat aatgtgcctg cattaataat
      181 aggggtatgg atttttgcta ttgtctttat aagcttggta agaagtcttt tctgttggaa
      241 aataattaag cggggagtgg aacaaaataa aatacaatcc actttgctca agttttttct
      301 tcttactttt gtcatgggca gtatatgggg ggcgtgttac ttcattactt taccttattt
      361 aaatgacctt catgaattca ttattatttt ggtatttggc ggaatgtgcg caggcgcaat
      421 tgcctctctc tctgtttatt tgccagctta ttacgcctat gtttttccaa tgcttatacc
      481 agtcattatt tataattatg ccttgttaga tctcgataga acgattttag caaccatgtt
      541 tctgattttt attgctatgc ttattatttc ggcaaggatt aataatagat tactcaatca
      601 aaattttcga ttgttcaatg aaaaagaact attaattagt gaattgaaag tgatgacaat
      661 aactgattct ttgacaggct tatataacag gagatatttt gacacgctat tcccgcagga
      721 gtttaatcga gcaagacgta atcagtattt tttaagcctg gtatcaatag atgcagataa
      781 ttttaaatta atcaatgata atttgggaca tccttttggt gataaagtcc ttatcagtat
      841 cgctgattta ttaaaaaaaa tatttcgacg ttcgaacgat atactgtttc gtcttggggg
      901 agatgagttt gcaataatta tcgctaatca gccgattaaa gacacaatat ctatttgtca
      961 atcaattaaa aatcctttta aaacaga
//
LOCUS       DATNSN010000346          985 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.350, whole genome shotgun sequence.
ACCESSION   DATNSN010000346 DATNSN010000000
VERSION     DATNSN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.350"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          311 a          168 c          208 g          298 t
ORIGIN      
        1 aaaaaattat tggtaaaact tgattatcca gacaatattg agattgaatc atacccctct
       61 tcatttgttg gttaagcagg tgtagaggac caaaggctca gatcttgctt tgccaatcag
      121 gctaccttgc tctcttaatc tgtattttct catcattcca tccgataaaa acttatttgc
      181 cctgtctttg gtttagtgtt gaacgagcgg ctcactcaaa gatgaaacct ggccaaggcc
      241 ataaagctcg aaatagggga caaatcaatc gttataaggc aaacgatctc gccgatctac
      301 aatagttttt gagtgcttat ttcctccttg ttttgatgat taataaaaac gacaagacaa
      361 tggctattgt catacaagtc catggtcaaa ttgaaataaa ttgacttaaa ctcccgaggg
      421 agttgccctg tcggatgaca gagattgcta gatggttttc cctactgaac gtccagccac
      481 agggttaggg taatgaagtg gcttagcata agacgtcgat tgacgtttcg ccggagtttc
      541 accagctcat cagtcatgct tgtgatttaa aattagcaaa gcggtgcttg cttagcaaga
      601 gattttagaa ataattttgt ccaatcccga ttggcgggat cggacaaaat gctgaaatgt
      661 gttgatttta cgcggtaaaa tcaaggtgat ttttctgcgt cactgctggc gatttgcagt
      721 gacgcagaaa atcttaacag gctcttatgt gctataatta aacaaaaatt aaaggagtag
      781 taggcaaaat aattaaatta tatttgtttg aatgaaattc acggaatgaa agatgagtat
      841 agttattggt agacagttta ggtggcaaag cgagtatccg aataattgtc cagggagaga
      901 tgctagtact ataaaattag aagtttatag atttgtaaag cataatcctc ctattgaaaa
      961 agattttata ccttatgcaa aggaa
//
LOCUS       DATNSN010000347          963 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.352, whole genome shotgun sequence.
ACCESSION   DATNSN010000347 DATNSN010000000
VERSION     DATNSN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.352"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            16..>963
                     /locus_tag="VAH76_003208"
     CDS             16..>963
                     /locus_tag="VAH76_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEO1387770.1"
                     /translation="MRKVIKYLSIILGSLFVSGFLLLFFSPKPSLLEEFNFSKAVYDD
                     NQQLLRLILSEDDKYRLYVPLSQIPKELMEATLLQEDQYFPWHKGVNPFSMVKAIWQT
                     YVVKSRRIGASTITMQVARIRFGINSKKISGKLWQIIRALQIERHYDKDKILEAYFNL
                     APYGGNIEGVGAASLIYFNKPVSKLGLPEALTLSIIPQNPVKRTSKNSELKKIRNRLF
                     ARWLKQHPQDQEKQPLFGLPLVMQTNQNLPFLAPHFVNMVLKENPGLSQSISTTLDYR
                     LQLIVERITRHYLARKKMFGVHNAAVLLVDSRTMSIKALM"
BASE COUNT          321 a          157 c          190 g          295 t
ORIGIN      
        1 tccctgttgt gggcaatgag aaaagtaata aaatatctta gcataatctt gggttcgcta
       61 tttgtcagtg ggtttttatt attatttttt tctcccaaac cttctttatt agaagaattc
      121 aatttttcta aagcggtata tgatgataat caacaattat taagattaat tttaagtgaa
      181 gatgacaaat acagacttta tgtaccgtta tctcaaatac ccaaggagct aatggaggcc
      241 acacttttac aagaagatca gtattttccc tggcataaag gagtaaaccc tttttccatg
      301 gtgaaagcga tttggcaaac ttatgtggtt aaatcgcgca gaatcggggc ttcaacaatc
      361 acaatgcagg tggcacgaat tcgttttgga attaactcca agaaaatttc tggaaaatta
      421 tggcaaatta ttagggcgtt acaaatagag cgtcattatg ataaagataa aattcttgag
      481 gcttatttca atttggcacc ttatggtgga aatatagagg gagtaggggc ggcgagctta
      541 atttatttta ataagcctgt tagtaaactt ggtttaccag aggcactaac tttaagcatt
      601 atccctcaaa atccagtaaa aaggacatca aaaaacagtg aattaaaaaa aataagaaat
      661 cgtttatttg ccagatggct gaagcagcat ccgcaagacc aggagaagca acctctgttt
      721 ggactgcctt tagttatgca aacgaatcaa aacctaccct tcctggctcc tcattttgta
      781 aatatggtac tgaaggaaaa tcctggttta tcacaaagta tttcaacaac tttggattac
      841 aggcttcagt taatagttga gcgaattaca aggcattatt tagcaagaaa aaaaatgttt
      901 ggtgttcaca acgcggcggt attacttgtg gatagccgta ctatgagtat taaagcgttg
      961 atg
//
LOCUS       DATNSN010000348          940 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.353, whole genome shotgun sequence.
ACCESSION   DATNSN010000348 DATNSN010000000
VERSION     DATNSN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.353"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            129..>940
                     /locus_tag="VAH76_003209"
     CDS             129..>940
                     /locus_tag="VAH76_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HEO1387771.1"
                     /translation="MLVGSIWFIKDRLQTLMLVQDIRRFLEELRESKTIIPCDKRLSA
                     ILQEHFSHRESHEELTSNDIQFVLQCFSERWIADSECDYLLYPSQANQVWIKLAHEIE
                     PFTDKNYLQILLPHITNQFDFNNLTPLTETVRLENFYLGYDGKTLYRKRGLCERLLDN
                     QFELSTCRTLKTKQCEVMTIEELTRLYRGKYCNGEFSIDKEKFDNFWDFLYKKTFPRM
                     QSKGEIPLEVLPHLLMLIESYYHLKNSGADIKLFTAEIHKFFKLLYQFKLEN"
BASE COUNT          324 a          134 c          155 g          327 t
ORIGIN      
        1 ttcacatgtt tttttctatt aattaaatcc tctattgacc aaagtacaat ctgatatcaa
       61 aataattcct atttattagc aagcatgaca ttgcttaggt tattccagtt caatagtatt
      121 gatagagaat gcttgttggt tcaatttggt ttattaaaga taggctgcag actttaatgt
      181 tagtacaaga cattcgtagg tttcttgaag agttgaggga atcgaaaact attattccct
      241 gtgataaaag attatccgct attttacaag aacatttctc ccatagagaa agtcatgaag
      301 agttaacctc caatgatatt caatttgttt tgcaatgttt tagcgaacgc tggattgctg
      361 actcagagtg tgattatctg ctttatcctt ctcaggcaaa ccaggtttgg atcaagttgg
      421 ctcatgagat agaaccattc acagataaaa attatttgca aattttgctg ccccacataa
      481 ccaatcagtt tgattttaat aatttaactc cattgactga aaccgttcga ttagaaaatt
      541 tttatcttgg atatgatgga aaaactttat atagaaaaag aggattatgt gagcgtttgc
      601 ttgataatca atttgagtta tccacttgca gaacgctaaa aaccaaacaa tgtgaagtaa
      661 tgacaattga agaattaaca aggttatata gaggaaaata ttgcaatgga gagttttcta
      721 ttgataaaga aaaatttgat aatttttggg attttctata caaaaaaact tttccccgta
      781 tgcagtccaa aggtgaaatt ccattagaag tacttcctca cttgcttatg ttaattgaaa
      841 gttactatca tttaaaaaat tctggggcag atattaaatt atttacagcg gagattcaca
      901 aattttttaa attattatat caatttaagt tagaaaatat
//
LOCUS       DATNSN010000349          933 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.354, whole genome shotgun sequence.
ACCESSION   DATNSN010000349 DATNSN010000000
VERSION     DATNSN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.354"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            39..>933
                     /gene="lbtU"
                     /locus_tag="VAH76_003210"
     CDS             39..>933
                     /gene="lbtU"
                     /locus_tag="VAH76_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin import outer membrane
                     protein LbtU"
                     /protein_id="HEO1387772.1"
                     /translation="MLNVIVLAFADAGDSLFKNEKLTYDGHLFFNATNSAIQGEKYLL
                     NQQLSNIKMGSELQVTDWNKMKGLLIYNTLPTPVAPQFYFEQFYDELQIESSNIFFAF
                     GKKWLTFGNYKSDLIYKPLTKALGQTNEVTAIIGYDSLYYANASFFKPYSRINTSSLP
                     LYYNLNTGVHNQSMDAGVSYLYSLAESQLFQYNKGFGGLLSQSLKTRVPGFATYFNLK
                     YKKTSTYLTYVTAITPFALEDMSYNNRRASPKALSIQNSYDVNIKKFSFKIIGFYDYT
                     FQALALGIPKQRLGLGLSATPF"
BASE COUNT          316 a          169 c          149 g          299 t
ORIGIN      
        1 gaaattatat taggacaaga attattttag gaatattaat gttgaatgtt atcgtgttag
       61 ctttcgcaga cgctggagac tctttattta aaaatgaaaa attgacctac gatggacatc
      121 tgttttttaa tgccaccaac tcagcaatac aaggtgaaaa atatttactg aatcagcagc
      181 tgtccaatat caaaatgggg tctgaattac aggtgactga ttggaacaaa atgaaaggct
      241 tgctaattta taacaccctg cctaccccag ttgctcctca attttatttt gagcaatttt
      301 atgacgaact ccaaatagag tcttcaaata ttttctttgc atttggaaaa aaatggctca
      361 catttggaaa ttataaaagt gatttaattt ataaaccact tacaaaagcc ttgggacaaa
      421 ccaacgaagt gaccgctatt attggatacg attccttgta ttatgctaac gcttcttttt
      481 ttaaacccta ttctcgcata aatacatcat cacttccgct ttattacaat ctgaatacag
      541 gggtacataa tcaatcaatg gatgcgggag tcagttattt atactctctg gcagaaagtc
      601 aattatttca atacaacaaa ggttttggcg ggcttttatc acaatcatta aaaacaaggg
      661 ttccaggatt tgcaacctat tttaatttaa aatataaaaa aacaagcact tatcttacct
      721 atgttactgc aatcaccccc tttgcattag aagacatgag ttataacaat cgaagagctt
      781 cgccaaaggc attgagtata caaaatagtt atgatgttaa tattaaaaaa ttttctttta
      841 aaatcattgg tttttatgac tacacttttc aagctctggc actcggaata ccgaaacaaa
      901 gactgggact gggattgtcc gcaacccctt ttc
//
LOCUS       DATNSN010000350          918 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.355, whole genome shotgun sequence.
ACCESSION   DATNSN010000350 DATNSN010000000
VERSION     DATNSN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.355"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>918
                     /locus_tag="VAH76_003211"
     CDS             <1..>918
                     /locus_tag="VAH76_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387773.1"
                     /translation="DTTNLYWNQLHEYSTLLNQCRTTLQKSHRLTEEGQLALRCFNNA
                     IQKMPSKKHIINALKILNKSNNKTEVIEKIGFVSPHGREACMQLTCWGKKFFYIPRSE
                     EMYHFDYDPKEDLNDTGGNCFGESMMFIHFLSKGVIRWLCPEAGLINFQLDQTRNLKF
                     KKTILGEGETVVSESSPHDSIQWEDMMPVLLDNPYFKPGSLCGVIFSMNDYTKAQREF
                     TAGHIIVVAKLDTNLNPYKYIVYEKDFGAFGLTDDESLEYLISEQILPLYSGMNYSKI
                     KLVKYGEASTETYDLLNEIKPQTSIPQKES"
BASE COUNT          318 a          142 c          183 g          275 t
ORIGIN      
        1 ggataccacc aatctgtatt ggaaccaatt acacgaatac tcaacattat taaatcagtg
       61 tcgcactact ttgcaaaagt ctcatcgact tactgaagaa ggacaattgg cgcttcgctg
      121 ttttaataac gcgattcaga aaatgccatc caagaaacat attattaatg cgttaaaaat
      181 attgaataag tcaaataaca agacagaagt aattgaaaaa ataggctttg tttcacctca
      241 tggtagggaa gcctgtatgc aactaacttg ttgggggaaa aaattttttt acatacctcg
      301 aagtgaggaa atgtatcatt ttgattatga tcctaaagag gatttgaacg atactggcgg
      361 taattgtttt ggtgaatcaa tgatgtttat tcatttttta tctaagggag taattagatg
      421 gttgtgtccc gaagcagggt taattaattt tcagttagat cagacaagaa atttaaagtt
      481 taaaaaaaca attctaggtg aaggggaaac ggtggtatcg gagagtagcc cccatgactc
      541 tatacaatgg gaagatatga tgcctgtgct tttggataac ccttacttta aaccaggcag
      601 cctctgcggt gtcattttct cgatgaatga ttacacaaaa gcacaaagag agtttaccgc
      661 agggcacatt atagtagttg caaagctgga tactaacttg aatccgtaca agtacattgt
      721 atatgaaaaa gattttggtg catttggatt aactgatgat gaatctttgg aatacctaat
      781 tagtgaacaa atattaccac tctatagtgg gatgaattat tcaaaaatca aattggttaa
      841 atatggtgag gcttcaactg agacctatga tcttttaaat gaaattaagc ctcagacaag
      901 tattccacaa aaagagtc
//
LOCUS       DATNSN010000351          911 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.356, whole genome shotgun sequence.
ACCESSION   DATNSN010000351 DATNSN010000000
VERSION     DATNSN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.356"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(128..892)
                     /locus_tag="VAH76_003212"
     CDS             complement(128..892)
                     /locus_tag="VAH76_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/III secretion system protein"
                     /protein_id="HEO1387774.1"
                     /translation="MIKRWLLVILLISGNAFSQQMITKVIELNYLQADKVIQLIQPLL
                     QTDEKVSGSGQTLIVNVSPQTLTQIRNVLHKIDVPPVVFNITVYQGDPEWLSSQDGND
                     VVYSTQPQYEVQRSQSVKVMSGESAFVATGEQVPVVTSVGVGLLATGVSYEQHNIQNG
                     LLVHPVLQGSQVKLTVRRVREQENVESDQQFDNQQIDTTIMAPLNKWVPLGTAEGVQN
                     TDSSSTSYTAGRPFSQNSTLYIKISVVKSTPSGTTK"
BASE COUNT          274 a          181 c          166 g          290 t
ORIGIN      
        1 atcaaggcaa aaatattttt aatgaaggga ttaggagcta gttaatcagt aaatcctggc
       61 ttattctcca gttgagggaa gaaaagctca ttaaggtaaa atgattatga aacgcatttc
      121 catcatttta tttggtggtc ccagaagggg tggattttac aactgatatc ttaatgtata
      181 aagttgaatt ttgagaaaaa ggccttccag ctgtataaga agttgaagaa gaatcggtat
      241 tttgaacccc ctctgctgta cctaatggaa cccatttatt caatggcgcc atgatcgtag
      301 tatctatttg ttgattatca aattgttgat cactctctac attttcctgc tctcgcactc
      361 ttctcaccgt caatttgacc tgtgatcctt gcaaaacagg atgtactagc aaaccgtttt
      421 ggatattatg ctgctcataa gatactcctg tggctaaaag accaacacca actgaagtaa
      481 ctactggtac ttgttcaccg gtcgcaacaa aagcggattc cccactcatg actttaacgg
      541 attgactgcg ctgcacttcg tattgtggtt gtgtgctgta aacgacatca ttcccatcct
      601 gtgagcttaa ccattcagga tccccttgat aaacagtaat attaaaaaca acaggaggaa
      661 catctatttt atgtaatacg tttctgattt gcgttaaagt ttggggactc acattaacaa
      721 ttagtgtttg ccctgaacca cttactttct catcagtctg caataatggt tggattaact
      781 gaataacctt atctgcttgc aaataattca attcgataac cttggttatc atctgctggg
      841 aaaaagcgtt gccagaaatc aataatatta ctaatagcca tcgcttaatc atagaaacac
      901 tcctgttgtt t
//
LOCUS       DATNSN010000352          882 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.357, whole genome shotgun sequence.
ACCESSION   DATNSN010000352 DATNSN010000000
VERSION     DATNSN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.357"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            115..>882
                     /locus_tag="VAH76_003213"
     CDS             115..>882
                     /locus_tag="VAH76_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEO1387775.1"
                     /translation="MINIIKNRNKEKHTTSNELQSKIEKEQVKLLFDQVGMAISGEAL
                     AVTILPIALWSVTNHELLIIWMIFTYVFSDLYKSMLLFYYNKQPTLFSTENWLFLFNL
                     GVVSSGLAWGFASSIMIPASSTLHQIFVVFLITGVTAAANSLYSPVRLSYFLFLVTAF
                     VPFTIWLFSKGQVYLLLGFCGIIYMGIMLFISYYSNRILINTLKMRFKISNLNSTKDI
                     LEKKVADRTKELEKILALTKSTLESTADGILVVDLKGN"
BASE COUNT          269 a          143 c          141 g          329 t
ORIGIN      
        1 attcatttgg atttcacaat gcacttagtt ttattttcta actgtttacg aggaaaacaa
       61 tcaacattca tgctcgcttc gctttttaac ataccatttt actaactact tgttatgatt
      121 aatattatta aaaacagaaa caaggaaaag catactactt ccaatgagtt gcaatcaaaa
      181 attgagaagg agcaggtcaa actgcttttt gatcaagttg gtatggctat atcaggcgaa
      241 gctttagcag taactatact gcctattgca ttatggtctg ttacaaatca tgagctgctt
      301 attatttgga tgatttttac ttatgttttt tcagatcttt ataaaagcat gctgctcttc
      361 tattacaata aacaaccaac attattttca actgaaaatt ggttgttcct ttttaacttg
      421 ggtgttgtaa gttctggctt ggcatgggga tttgccagtt caattatgat tccagcctct
      481 agtacattgc atcaaatctt tgtcgttttt cttattacag gagttacagc agcagccaac
      541 tcactctatt cacctgtgcg attatcctat tttttatttc tggtcactgc ttttgttcct
      601 ttcactatat ggttattttc aaaaggacaa gtttatttat tgttggggtt ttgtggcatt
      661 atttatatgg ggattatgtt atttatttca tattactcaa acaggatttt gattaatacc
      721 ttaaaaatgc gctttaaaat ctctaattta aattcaacta aagatatttt agaaaaaaaa
      781 gtagcagaca gaaccaaaga attggaaaaa atcctggctt taaccaaatc aactttagag
      841 tcaacagcgg atggtatttt agttgtcgat ctcaaaggta at
//
LOCUS       DATNSN010000353          881 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.358, whole genome shotgun sequence.
ACCESSION   DATNSN010000353 DATNSN010000000
VERSION     DATNSN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.358"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>881
                     /locus_tag="VAH76_003214"
     CDS             <1..>881
                     /locus_tag="VAH76_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISLpn11 family transposase"
                     /protein_id="HEO1387776.1"
                     /translation="RSYLCIKAHKYYCPACGRYFNQRFPGIGKYQRASESLRKQVFHY
                     HSKGVSQKDLARDLKLGKSTVERWYHYGYELRYKKIATRSCPRVLGLDEHSFNRKVGY
                     ATTFCDLAKHKIFDVVEGRSEKDLEGYLNTLEGKDKVQVVCIDLSSSYRKLIRRYFPN
                     AMIVADRFHVIRLLNQFCLQTYQQIDPEMKYQRGLLAALRTNPNNLTVKRLNRRERYL
                     QQQPVIAAIYYFKQRLHRLLMRKHRTAKQCTRLIPLFLKLVASLKESPFESLKTLGRT
                     LYQWREEVVRMWRFTKN"
BASE COUNT          263 a          158 c          201 g          259 t
ORIGIN      
        1 acgcagttac ttgtgtatca aagctcataa atattattgt cctgcgtgtg gtcgctattt
       61 taatcagcgt tttcctggga taggcaagta tcaaagagcg agcgagagtt tgcgtaaaca
      121 ggtatttcat tatcacagca aaggagtgag tcaaaaggat ttggctcgtg atttaaagct
      181 gggtaagtcg actgtagagc gctggtatca ttatggttat gagcttcgct ataagaagat
      241 agcgacacgt tcctgtcccc gagtattagg acttgatgaa cattcattta atcggaaagt
      301 gggttatgcc acaacctttt gtgacttggc caaacataaa atatttgatg tggttgaggg
      361 acgttcagaa aaggatttag aaggttatct aaacacgtta gaaggaaaag ataaggtaca
      421 ggtagtctgt attgatttaa gttccagtta tcgcaagtta atcaggcgtt actttcccaa
      481 tgcgatgatt gttgccgatc gctttcatgt gatacgtttg ttgaatcaat tctgtttgca
      541 gacttatcag cagattgacc ctgaaatgaa atatcagaga ggtctcttgg cggcattgag
      601 aaccaatccc aataatctca ctgtaaaacg actcaataga cgggaacgtt atttgcaaca
      661 acagcctgtc attgctgcaa tatattactt taaacagcga ttacatcgat tactcatgcg
      721 aaaacatcgc accgcaaagc agtgtacgcg tttaatccct ctctttctta aactggttgc
      781 cagtctgaag gaaagtcctt ttgaatcact caagactctg ggtaggacat tatatcaatg
      841 gcgggaagaa gtggttagaa tgtggcggtt tactaaaaac a
//
LOCUS       DATNSN010000354          876 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.359, whole genome shotgun sequence.
ACCESSION   DATNSN010000354 DATNSN010000000
VERSION     DATNSN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.359"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            33..>876
                     /locus_tag="VAH76_003215"
     CDS             33..>876
                     /locus_tag="VAH76_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEO1387777.1"
                     /translation="MLNLAEELGNVSKACKVMGLSRDTFYRYKSAVESGGVDALFDQT
                     RRKPNHKNRVDESIELAVKEYAIEYPAHGQQRTSNELRKKGIFVSGSGVRSVWLRHDL
                     ANFKDRLKALEAKVASEGIVLTEAQVIALERKKFDDEACGEIETAHPGYLGSQDTFYV
                     GTLKGVDRIYQQTFVDTYSKVAFAKLYTTKTPITSADILNDKVLPFFEHHQLPMLRIL
                     TDRGTEYCGKVEQHDYQLYLAINNIDHTKTKAQSPQTNGICERFHKTILQEFYQVTFR
                     KKLYD"
BASE COUNT          296 a          160 c          182 g          238 t
ORIGIN      
        1 atagcgttaa aataattaaa cataaagttg gattgcttaa tttagctgaa gaattaggga
       61 atgtatcgaa agcctgtaaa gtaatgggat tatcccgcga tactttttat cgttacaaat
      121 cagcagtaga gtctggtgga gttgatgcat tattcgatca aactcgcaga aaacctaatc
      181 acaagaatcg agttgatgag tctattgaac tagcagtaaa ggaatatgct atagaatacc
      241 ctgctcatgg ccaacagcgc acaagtaatg aactacgaaa aaaaggtatc tttgtttctg
      301 gcagtggagt acgtagcgtt tggttaaggc atgatttggc caatttcaaa gaccgattaa
      361 aagcacttga agccaaggtg gcatcagagg gcattgtatt aaccgaagcc caggttattg
      421 ctttggaacg gaagaaattt gatgatgaag cctgcggtga aattgaaaca gctcaccctg
      481 gttacttggg ttctcaagac acattttatg tcggaacact caagggtgta gacaggatct
      541 atcagcaaac attcgttgat acttatagca aagtagcatt tgctaagcta tatacaacga
      601 aaactccaat cacatcagct gatattctaa atgataaagt tctacctttc tttgagcacc
      661 atcaattacc gatgttgcgc attttaactg ataggggcac agagtattgc ggaaaggtcg
      721 agcaacatga ttaccagctt tatttagcga tcaacaatat cgatcatacg aaaaccaaag
      781 cgcaatcgcc acaaaccaat ggtatctgcg agcggttcca taaaacaatt ttacaagagt
      841 tttatcaagt tacattccgt aagaaacttt acgata
//
LOCUS       DATNSN010000355          836 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.360, whole genome shotgun sequence.
ACCESSION   DATNSN010000355 DATNSN010000000
VERSION     DATNSN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.360"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>836
                     /locus_tag="VAH76_003216"
     CDS             <1..>836
                     /locus_tag="VAH76_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO1387778.1"
                     /translation="LSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESG
                     VYLPIIAHQKALARAQNNRPNKLKNNGILRDYVVRSLVNKGWSPEQISGRMKYQKLSF
                     YVCPETIYRYVYQSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQRPEEIDS
                     RERFGHWEGDTIEFKGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLP
                     QKMFKTITFDQGSEFADHEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNA
                     KI"
BASE COUNT          314 a          123 c          159 g          240 t
ORIGIN      
        1 attatctgta acagatcgac gccgtttatt tgtttttctg gatatgggat tatctgtacg
       61 cgagatcacg cataaattaa agaggcatcg ttcaacaata tacagagaac taaataggaa
      121 taatgaatcg ggagtttatt taccgattat agcccatcag aaggctttag ctagagctca
      181 aaataatcgc cctaataaac taaaaaataa tggaatttta cgtgattatg ttgttcggtc
      241 attagtcaat aaaggatgga gtcccgaaca gatatctggc cggatgaaat atcaaaaatt
      301 gagtttttat gtttgtcctg aaactattta tcgttatgtt tatcaatcta atgataaggc
      361 gctttatcat tcactagcct ttaaaaaacc tagaagacaa aagctcttta ggcgaaagca
      421 tagaacatgt cgttatggca ctccttatct gattactcaa agacctgaag aaatcgattc
      481 aagggaacgt tttggacact gggaaggcga tacaattgaa tttaaaggga ataaaagcga
      541 agtgattacg acacttgtgg agcgtaaaac cagaatggtt tatctcataa aaaatacgag
      601 caaacattca cgaggagtga tggaaaaaat aaataagaaa tttgaaaaat taccacaaaa
      661 aatgtttaaa acaatcactt ttgatcaggg ttctgagttt gctgatcatg aatatcttga
      721 gaaaaacatg aaatgtgaag tttattattg tgatgttcac tccccatggc aaaaaggaag
      781 taatgaaaat atgaatggtc gaataagaaa atacttacct aaaaatgcta aaatta
//
LOCUS       DATNSN010000356          834 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.361, whole genome shotgun sequence.
ACCESSION   DATNSN010000356 DATNSN010000000
VERSION     DATNSN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.361"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            139..831
                     /locus_tag="VAH76_003217"
     CDS             139..831
                     /locus_tag="VAH76_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEO1387779.1"
                     /translation="MTMKFPFQIIDLTHPVSPDSPGWDLDCGFNHSNFIDYEDCTSEI
                     KFRVQQIHSPLGIGTHMDAPAHCIPGAASITDLDLHRNYLLTQCVLINVSHQTDEHYQ
                     ITKQDILDFEARYGRLKKNAFVLFYTGWEKHWPNPDKYRNNLVFPSISVHAAQLLLER
                     NIAGIGIDTLSPDTADSGYPVHQLLLQAGKYIIENVANANFLPPIGAWTLALPWKIIN
                     ATESPVQLIGLV"
BASE COUNT          253 a          177 c          147 g          257 t
ORIGIN      
        1 cattattagc ttgcatgatc attattataa taaaaggata aactgacgct cctttgcaaa
       61 acctgaatta aaaattaatt tcattgatcc tcattctttt tatgataatt taattgaaac
      121 aatccatcgc tctctcaaat gactatgaaa tttccttttc aaataatcga tttaacgcac
      181 cctgtttcac ctgactctcc tggttgggat ctggattgtg gatttaacca tagtaatttt
      241 attgattatg aagactgtac atcggaaatc aaattcagag ttcagcaaat acactcaccc
      301 ttggggattg gaacccatat ggatgctccg gctcattgta ttccaggagc tgcctctatt
      361 actgatctcg atctgcaccg taattacctg ctcacacaat gtgtgctgat taatgtcagt
      421 catcaaacag atgaacatta tcagattaca aaacaggata ttctggattt cgaagccaga
      481 tatgggaggt taaaaaaaaa tgcgtttgtg cttttttata ctggctggga gaaacattgg
      541 cctaatcccg acaaatatcg caacaatttg gtttttccaa gcattagtgt ccacgcagcc
      601 caactattgc ttgaaagaaa tattgctggc attgggatag acacactttc tcccgatacc
      661 gcagactcag gttacccggt gcatcaactg ttattacaag caggtaaata cattattgaa
      721 aacgttgcca atgcgaattt cctgccaccc attggcgcct ggactcttgc tttaccatgg
      781 aaaataatca atgccactga atccccagtc caattgattg gactggtatg ataa
//
LOCUS       DATNSN010000357          818 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.362, whole genome shotgun sequence.
ACCESSION   DATNSN010000357 DATNSN010000000
VERSION     DATNSN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.362"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            61..>818
                     /locus_tag="VAH76_003218"
     CDS             61..>818
                     /locus_tag="VAH76_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO1387780.1"
                     /translation="MDLRKINLNLLLHLDTLLNELSVSKAAEKSFMSQTAMSHILKQL
                     RDIFDDPLFIRKPHGLQPTQKALDLAPKIKKFLNYSNDIFEEDTFNPGNDNLFLKTAS
                     VGTGEYFILPRLCAHLAKNAPKFVLKISSLSEYLSLEYLLMSELDFAIGPGFVETGNN
                     IIRELLFEEEAVCVMHKSHPLANQELTQDMYLQAEHVDIQFSYMGNENILYKALQGYH
                     QRNIKVIVPNIISALEVVYHTNFITTVPRTLAVT"
BASE COUNT          279 a          136 c          148 g          255 t
ORIGIN      
        1 tgggcttatt cagttaccct ggataggagt ataaaaaagt taatttggat ttaatgaaaa
       61 atggatctca gaaaaattaa tctcaacttg ttattgcatc ttgatacatt gttaaatgag
      121 ctctcagtat ccaaagcagc tgagaaatct ttcatgagcc agactgcaat gagccatata
      181 ttaaaacaat tacgtgatat attcgatgac cctttgttta ttaggaaacc gcacgggctt
      241 caaccaacac aaaaggcact tgatctggcc cccaagatta aaaaattttt aaattactcc
      301 aatgatattt ttgaagaaga tactttcaat ccaggaaatg acaatttatt tttaaagact
      361 gcatcagtag gtacagggga atattttatt ttacccaggt tatgtgctca tttggctaaa
      421 aatgctccga aatttgtatt aaagatttct tcactgtcag agtacttgag tctcgagtat
      481 ttattaatgt ccgaattaga ctttgcaata ggaccaggtt ttgttgaaac ggggaataac
      541 atcatcagag aattattgtt tgaagaggag gccgtgtgcg tcatgcacaa atctcatcct
      601 ctcgcaaatc aggagttaac tcaagatatg tacttgcaag ctgaacatgt tgacatacaa
      661 tttagttaca tgggaaatga aaatatacta tataaagcgt tacaaggata tcatcaaaga
      721 aatatcaaag tgatagtacc caatatcatt agcgcattgg aagtggttta tcatacgaat
      781 ttcattacaa ctgtaccgag aactctagct gttacttt
//
LOCUS       DATNSN010000358          809 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.363, whole genome shotgun sequence.
ACCESSION   DATNSN010000358 DATNSN010000000
VERSION     DATNSN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.363"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            47..538
                     /locus_tag="VAH76_003219"
     CDS             47..538
                     /locus_tag="VAH76_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387781.1"
                     /translation="MKKWNPFLLLIYLSLFLGPAHAVTSLLTNFTQLVRAVERGDDVK
                     AIIRLDRCQITDPALQTQLVQNLDGASTRFNFTEYLHFRTRINDQLRDTVTTVSNKIF
                     EYSPGVFLSASGRLNVFEDNTATLRVNFYNPALGTSVLIIDWLCDISNGNDENGLFLY
                     NSP"
BASE COUNT          247 a          133 c          144 g          285 t
ORIGIN      
        1 tgttcttatg gattaagcaa ataacgaatc aataagaggg gacgttatga agaaatggaa
       61 tccgtttctc ttgttaattt atctgtcttt attcctggga cccgcacatg cggtcacttc
      121 gttgttgact aatttcacgc aattggtcag ggcggttgaa cgaggtgatg acgtcaaggc
      181 cattatacga ttggatcgat gccaaattac agatcccgca ctgcaaactc aattagtaca
      241 gaatttggat ggcgcttcaa caagattcaa ttttacagag tatttgcatt ttcgaaccag
      301 aattaatgat caattacgag atacggtaac tactgtctca aacaagatat ttgagtattc
      361 acctggcgtg tttttatctg cttctggccg tttaaatgtc tttgaagata atactgctac
      421 tttgcgtgtc aatttctata accctgcttt aggcacaagt gtgttaatta tcgattggct
      481 ttgcgatatc agtaatggca atgatgaaaa tggattgttt ttatataata gtccttaagg
      541 cctgaaggta taaaatctac ccctttcatt tggctagctt cttaatgatt gttcatattc
      601 acctggtctt cttaagattt atcttagtaa acatgtatca aacgactatt aagcaatcaa
      661 ttctgcaatt ttatttttca aaatctcttc aaattgttgt aataatagtt aaaattgaaa
      721 aaatatatca atattgatca tatctagaat aatttaaact attcatgtaa taatgacttg
      781 tctagggaaa gaatttttag tgcttcatg
//
LOCUS       DATNSN010000359          794 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.364, whole genome shotgun sequence.
ACCESSION   DATNSN010000359 DATNSN010000000
VERSION     DATNSN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.364"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            71..316
                     /locus_tag="VAH76_003220"
     CDS             71..316
                     /locus_tag="VAH76_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1387782.1"
                     /translation="MKEKHPNLIKIGKLIRDTRKEKGYSQEDFASQAQLGRTYMGRIE
                     RGEQNMTIQTLIQIALILGVEVATLIPKLNELTPPKA"
     gene            377..>794
                     /locus_tag="VAH76_003221"
     CDS             377..>794
                     /locus_tag="VAH76_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387783.1"
                     /translation="MMNEIRSEKEIFEDLEALCCSDGYVHVIAYFCFKDNTILASENG
                     QMISSNENPSNRLIRTEIATLIGLMVKKEIKLPIPPPDIFDTYINQTQALLHELHQTM
                     YPSIGQSTEEVSLKKRMDLFTMGTFLKEGIFYAGESA"
BASE COUNT          309 a          124 c          133 g          228 t
ORIGIN      
        1 caaaattaac caacaaaatg ccactcatta ggtgtggtct gattaggtgt gaatattgtc
       61 tactttctta atgaaagaaa aacatcctaa tcttataaaa ataggcaaat taatcaggga
      121 taccagaaaa gaaaaagggt attctcaaga agattttgct tcccaagctc aattagggcg
      181 tacctatatg ggtagaattg aaaggggtga acaaaacatg actattcaaa cacttataca
      241 aatagcatta attttaggag tggaagttgc tacacttatc cctaaattga acgagcttac
      301 tccccctaaa gcttgaattt ctaaaagctc tgaaagtaac ttgattaaaa tcaataaaag
      361 tagatcaata atcctaatga tgaacgaaat aagaagtgaa aaagaaattt ttgaagattt
      421 agaagcatta tgttgttctg atggatacgt tcatgtaata gcctattttt gttttaaaga
      481 taatacaatc ttggcatctg aaaacggtca aatgatatct tcgaatgaaa atccttcaaa
      541 tagattaatt agaactgaaa tagcaacact gatcggccta atggtgaaaa aagaaataaa
      601 gttacctatt cccccaccgg atatttttga tacatatatt aatcaaactc aagcgttact
      661 tcatgagcta caccaaacaa tgtatccatc aataggacaa tctacagaag aagtaagtct
      721 taaaaaaaga atggacttat ttacgatggg cacattccta aaggaaggaa ttttttatgc
      781 aggagaatct gctt
//
LOCUS       DATNSN010000360          785 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.365, whole genome shotgun sequence.
ACCESSION   DATNSN010000360 DATNSN010000000
VERSION     DATNSN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.365"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..234
                     /locus_tag="VAH76_003222"
     CDS             <1..234
                     /locus_tag="VAH76_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387784.1"
                     /translation="PPEPEEVHWFKKFLRFITGNEKLLQNADEKRYDQQIKVLSDISD
                     LNKKLNSLPQEKNMPSEHEHSHEHGNENHMKLN"
     gene            complement(315..647)
                     /locus_tag="VAH76_003223"
     CDS             complement(315..647)
                     /locus_tag="VAH76_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387785.1"
                     /translation="MKIKCKYCQKRMKPNITVEERIVTTNLQIYYCSLCNKQLFILGG
                     YNYKPFWERPIYLWFLGPFWALMDLWEIAKTLIKEKERNYILLIIITGLFLVAILTLL
                     PKHGSYLY"
BASE COUNT          297 a          119 c          107 g          262 t
ORIGIN      
        1 cctcccgaac ctgaggaagt ccactggttc aaaaaatttc taaggtttat cacaggtaat
       61 gaaaaactat tgcaaaatgc tgatgagaaa cgttatgacc aacaaatcaa agtcctttct
      121 gatattagtg atttaaataa gaaactgaat tctttaccac aagaaaaaaa catgccatct
      181 gagcatgagc actctcatga gcatggaaat gaaaaccata tgaagctcaa ttagagaagc
      241 tatttttaga ttgccagggt aggtagcttt gtctatatct cagttaagtc tgattatcaa
      301 aataataatt agacttaata aagataagag ccatgctttg gtagaagggt aaggatagct
      361 actaaaaata acccagtaat aattattaat aagatataat tgcgctcttt ctcttttatt
      421 aacgttttag ctatttccca taaatccatt aaagcccaaa aaggtcctaa aaaccataag
      481 tatattggtc tttcccaaaa aggtttataa ttataaccac ctagaataaa tagttgtttg
      541 ttacataaac tacaataata aatttgtaga tttgtagtta ctattctttc ctcaacagtg
      601 atattaggtt tcattcgttt ttggcaatac ttacatttaa ttttcattca tatttaaaaa
      661 aataaataag tctattattt tatcattttg cttgatatat atcaaaaatc attattaata
      721 tgataaatat ttaaaaaata taaaataatt atggcaaacg tgatagggcc acctctcaat
      781 ttact
//
LOCUS       DATNSN010000361          776 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.367, whole genome shotgun sequence.
ACCESSION   DATNSN010000361 DATNSN010000000
VERSION     DATNSN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.367"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            74..>776
                     /locus_tag="VAH76_003224"
     CDS             74..>776
                     /locus_tag="VAH76_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO1387786.1"
                     /translation="MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHL
                     GLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVALQSSIMLMGLSSLCIGCLPAYDS
                     IGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
                     LMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKIL
                     FENYKKQLIRAVLLIA"
BASE COUNT          199 a          145 c          155 g          277 t
ORIGIN      
        1 attacataga ataatgagat caatttaaag taaaatctct ctacatctct ttaatattct
       61 attgatttat tgcatggaaa ataagaagaa aagagtcgtt gttgcaaccg ccggaacttt
      121 tctggaatgg gctgagttta cttattacgc ctatatcgca aatattatgt cgggatcgtt
      181 ttttccaaaa ttaggaaatc atcttggttt gatagcaacc ttttctgttt tcgcgctcag
      241 ttactttttc cgtcctttag gagccctctt ttttggatat cttggagaca agatggggcg
      301 tcgggttgcc ctgcaatctt ctattatgct gatggggtta tcttcactat gcattggttg
      361 tcttcctgct tacgatagta ttggtatctt ggctccaatt cttttactga tattccgttg
      421 tgttcaaggc tttgccgtgt ctggtgaatt taatggcagc gcgatctatc tgattgaaca
      481 tgattcacaa aaaccatgcc aagccggtag ctggactgga tttgcttccg cattgggtat
      541 gatgtttggc ggattgatgt ctactttaat ttatctacct cacatgcctg agtgggcatg
      601 gcgtatccct ttcctgttag gaactttatc ctgcttttgt gcgatgtatt ttcgtaaaaa
      661 tctgagtgaa tcaccgccct accttacgct aagtaacgaa aaatcatcca atccactgaa
      721 aattttattt gaaaattata aaaaacaatt aattagggca gtgttattaa tagctg
//
LOCUS       DATNSN010000362          755 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.368, whole genome shotgun sequence.
ACCESSION   DATNSN010000362 DATNSN010000000
VERSION     DATNSN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.368"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..674
                     /locus_tag="VAH76_003225"
     CDS             <1..674
                     /locus_tag="VAH76_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387787.1"
                     /translation="GIFWKSRLKNIEKWYGYDKAYVAMNLSTDVPSYPLLTTTIPNVN
                     FINLLNRLKKNLPTLEQEEHAFIETLESFNHNKIFQLLVLSNFTESGLISRENELKLY
                     LELCHRHCPPGSIIIVKPHILSNFAFLEALKSSLKNYQVVLFPKNLRCIPLEMLTGLI
                     DKCNIISVSSSSIFLSYLYGRKKIVHALTLNDIKEFFYKSSYQQMVESTQNIMNTLHG
                     FLKSY"
BASE COUNT          263 a          113 c          105 g          274 t
ORIGIN      
        1 aaggtatttt ctggaagagt aggcttaaaa acatagaaaa atggtatggg tatgataaag
       61 cttatgtggc aatgaattta agtacagatg ttccaagtta tcctttatta acaaccacaa
      121 tcccaaatgt aaattttatt aatttgctta acagattaaa aaaaaatctc cctactcttg
      181 aacaggagga gcatgctttt attgaaactt tagagtcgtt taatcataat aaaatatttc
      241 aactgcttgt gcttagcaat tttactgaaa gtggattaat ttcaagggag aatgagctta
      301 agttatatct tgaactatgt catcgccact gtcctccagg atctattatc attgtcaagc
      361 cacatatatt atcaaacttt gcttttcttg aagcattgaa aagtagcctt aaaaattatc
      421 aggtagttct tttccccaaa aatttacgat gtattccatt ggaaatgtta accggactta
      481 ttgacaaatg taatattatt tctgtttcct cgtcctctat ttttttatcc tatctatacg
      541 gtaggaaaaa aattgtacat gccttaactt taaatgatat aaaagaattt ttttataaat
      601 ccagttatca gcaaatggtc gagtcaactc agaatataat gaatactttg cacggctttc
      661 taaaatcata ttgatattac ttaaaattgt aaggaaaaat aatttatcta acttaaatta
      721 atttcattac tcatattttt tatatataat ttcgc
//
LOCUS       DATNSN010000363          726 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.369, whole genome shotgun sequence.
ACCESSION   DATNSN010000363 DATNSN010000000
VERSION     DATNSN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.369"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(161..634)
                     /locus_tag="VAH76_003226"
     CDS             complement(161..634)
                     /locus_tag="VAH76_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387788.1"
                     /translation="MPLTPPASPKTEKRSEEDNQQTNQQGNEQGNQQPRSSILTLFGF
                     TPNALVMPCPHGHGHFHIMDIQADDTLVFATMNGSPEIMSLLALQFLMQDMKLNVDDE
                     LAAETDAKEAEEKEEPELSSSESVNEEKEESEFSSSESESEETEEENEESNRFTM"
BASE COUNT          202 a          133 c          133 g          258 t
ORIGIN      
        1 tcagtgatat atagtttatt accgagatta attgctctcg tttgattatt gggatattta
       61 ttctgataag gattgacaat atttgagaca gaggaattaa aaaaatcccg tatttacctc
      121 aggtaaatac ggggtgtccc ttcaaaagag ggcaggcata ttacattgta aaacggtttg
      181 actcttcgtt ttcttcttcc gtttcttcac tctcagattc agaagatgaa aattctgatt
      241 cttctttttc ttcattcact gattcagaag atgacagttc tggttcttct ttttcttccg
      301 cttctttcgc atctgtttct gctgccagtt cgtcatcaac atttaatttc atgtcttgca
      361 tcaggaattg aagcgccaat agactcatta tttccgggct gccattcata gtagcaaata
      421 ctaatgtatc atctgcctga atatccataa tatggaagtg accgtggcca tgagggcatg
      481 gcatgactag agcattagga gtaaaaccaa aaagagtcaa tatactgctt cttggttgtt
      541 gatttccttg ctcgtttcct tgttgattag tttgttgatt atcttcttca gaacgttttt
      601 cagtttttgg tgaagcgggg ggggttaaag gcataatcat tactcctaaa ctaaaattaa
      661 atccaaaaaa tactgcccaa aaaagataat ctaattaaga atctatcttt tcggcgtcaa
      721 gtttac
//
LOCUS       DATNSN010000364          725 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.370, whole genome shotgun sequence.
ACCESSION   DATNSN010000364 DATNSN010000000
VERSION     DATNSN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.370"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..300)
                     /locus_tag="VAH76_003227"
     CDS             complement(<1..300)
                     /locus_tag="VAH76_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEO1387789.1"
                     /translation="MLNMDILNINPETVCFIIDKAKEFHAKEEVTFSEQIPDSEYEYD
                     WSQILADHADDLSYIEVRNVIEHLDPNQQTDLLTLMYVGRGDFDISEWSKAHKEAK"
     gene            422..721
                     /locus_tag="VAH76_003228"
     CDS             422..721
                     /locus_tag="VAH76_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1387790.1"
                     /translation="MNVINILKALSNEKRLQIIRWLKEPDKHFSSSHCDVSKDGVCVG
                     LIEKKSGLSQSTVSQYLSQLQQAGLIFTERRGQWTYCKANANLINEFIKELKVMI"
BASE COUNT          247 a          119 c          117 g          242 t
ORIGIN      
        1 tttagcttcc ttatgagctt tgctccattc tgaaatatca aaatcgcccc ttcctacata
       61 cattaatgtc aacaagtcag tttgctgatt tggatcgaga tgttctatta cattcctaac
      121 ttctatatag cttagatcat cagcatgatc agctaaaatt tgagaccagt cgtattcata
      181 ttcagagtca ggaatttgtt cactgaatgt gacctcttct tttgcatgaa attcttttgc
      241 tttgtcaatg ataaaacaca ctgtttcagg gttaatattc aaaatatcca tattgagcat
      301 ttgtctctcc atttgtttta tttttaatat tagtatcggg caggttattt gaaatttcaa
      361 cacacatgac catgagcaat cactaaattg acatatcgca ataaaacgat ataattaaaa
      421 tatgaacgta ataaatatat taaaagcgct ttctaatgaa aagcgattac agataatcag
      481 atggctaaaa gagccagata aacatttcag ttcgtcgcac tgtgatgtga gcaaagatgg
      541 tgtttgtgtt ggcttaatag aaaaaaaatc aggcttatct caatccacgg tatctcaata
      601 tttatcacaa ttacagcaag ctggtttgat attcactgag cgtagaggac agtggaccta
      661 ttgtaaagcg aatgcaaact taattaatga atttataaaa gaattgaaag ttatgattta
      721 aaaaa
//
LOCUS       DATNSN010000365          725 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.371, whole genome shotgun sequence.
ACCESSION   DATNSN010000365 DATNSN010000000
VERSION     DATNSN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.371"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..429
                     /locus_tag="VAH76_003229"
     CDS             <1..429
                     /locus_tag="VAH76_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387791.1"
                     /translation="FCNSRSVPWVYQLSFVFYDYILDKEFEFITTKSGRDIFDAKKQL
                     VLKYIQKAADLYPRYEKEDFSDCHEMVNILLLSMRSEENNLQKRRNQDSSTSYMYSYF
                     TQTLYNASGKLMGKGQLGQKIDLLIEEFNERVVNKTPLYT"
     gene            complement(532..>725)
                     /locus_tag="VAH76_003230"
     CDS             complement(532..>725)
                     /locus_tag="VAH76_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387792.1"
                     /translation="KHLHHSIELIRNKPFHSDDRFYGQWSFEGHDPKRILEFIKSDDI
                     SGLMTILEDKKALPNNKPN"
BASE COUNT          240 a          117 c          135 g          233 t
ORIGIN      
        1 ttttgcaata gtagaagcgt accctgggtg tatcagcttt cctttgtatt ctatgactat
       61 atccttgata aggaatttga gtttataacc actaaaagcg gcagagatat ttttgatgca
      121 aagaaacagt tggttcttaa atacatccaa aaggcagcgg atctttatcc tcgctatgaa
      181 aaagaagatt tctctgattg ccatgagatg gtgaatattc tgttactcag catgcgttct
      241 gaagaaaata atttacaaaa gcgcagaaat caggatagta gtacaagtta tatgtactct
      301 tattttacac aaacacttta taatgcttcc gggaaattaa tggggaaggg gcaattaggg
      361 caaaaaatag atttattgat agaagaattt aatgaaagag tagtcaataa aacacccttg
      421 tatacttaat tctttatttt ccaaagcaag acagaaaggt cactggatta atagtaaaaa
      481 caccaattca agttgatttg ttaactcagt gacgccttgg atttaaacct gttaattcgg
      541 tttattattg ggaagcgcct ttttgtcttc taaaatagtc attaatcctg aaatatcatc
      601 gcttttaatg aattcgagga ttcttttggg atcatgccct tcaaaactcc actgtccata
      661 aaatcggtcg tcagaatgaa acggcttatt tctgattagc tcgatagaat gatgcagatg
      721 cttca
//
LOCUS       DATNSN010000366          707 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.372, whole genome shotgun sequence.
ACCESSION   DATNSN010000366 DATNSN010000000
VERSION     DATNSN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.372"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(265..>707)
                     /locus_tag="VAH76_003231"
     CDS             complement(265..>707)
                     /locus_tag="VAH76_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lpg2555 family Dot/Icm T4SS effector hydrolase"
                     /protein_id="HEO1387793.1"
                     /translation="EIEAAQSKGIPTIHATHNKYLQNTEQFFSGLIHQVDNMELSHIF
                     NKVAAEIRNNSRSISWGRQIAGFFSVHLPIAQSLENLASKLQRKSANEVSEEFDRYLK
                     KIAYDPNKKGDIAVSLTKAKTAIDKHISAPIEIKEGQLFLNRMG"
BASE COUNT          211 a          118 c          128 g          250 t
ORIGIN      
        1 attattcaat aagtcaacag cataaaatta aatcgcaact ggatgattga aatattgatt
       61 attacagaaa tcaatgtatt tctccttgga gcattgctat tgactttaga tatcgataca
      121 tatatctaaa tctcgagatt tgtttgcagg atctcacaaa ttttaaatcc cgaggacaag
      181 ccccgggatg tcgattgagg aagctaaagt gaatgacagt agttcctgga gcataccaag
      241 gcacctggat aatggaaagg attgttaacc cattctattg agaaatagtt gaccttcttt
      301 gatttcaatc ggggctgata tatgtttatc tattgctgtt ttagctttag ttaaacttac
      361 ggctatatcc ccttttttat ttggatcata agcaattttc ttaaggtatc gatcaaattc
      421 ttcgctaacc tcgttagcag attttctttg taatttagat gccaaatttt ctaaactctg
      481 tgctataggc aggtgaactg aaaagaaacc agcaatttgc cttccccagg aaattgaacg
      541 agaattgtta cgtatttcag ctgctacttt attaaaaata tgcgaaagtt ccatgttatc
      601 tacttgatga attaatcctg aaaaaaattg ctctgtattt tgcagatatt tattatgagt
      661 cgcatgtatt gtgggtattc cttttgactg tgcggcttct atttctg
//
LOCUS       DATNSN010000367          689 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.373, whole genome shotgun sequence.
ACCESSION   DATNSN010000367 DATNSN010000000
VERSION     DATNSN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.373"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>689
                     /locus_tag="VAH76_003232"
     CDS             <1..>689
                     /locus_tag="VAH76_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387794.1"
                     /translation="ADWGYNGFTQVGGWRHWHDVDHEWDWGFKLEGSYHFNTGNDINV
                     NWYHFDNDSDHWADFANWHNYNNKWDAVNAELGQFVDFSANKKMRFHGGVQYARIEAD
                     VNRYFNNFAFNGFNSKFNGFGPRTGLDMNYVFGNGFGVYAKGAAAILVGTSDFYDGIN
                     FITGSKNAIVPELEAKLGADYTYAMAQGDLTLDVGYMWFNYFNAMHNTGVFNGFETDF
                     AASGPYIGLKYV"
BASE COUNT          174 a          131 c          163 g          221 t
ORIGIN      
        1 gctgattggg gctacaatgg tttcacccaa gttggtggct ggcggcattg gcatgatgtt
       61 gaccatgagt gggattgggg cttcaaatta gaaggctctt atcacttcaa tactggtaat
      121 gacatcaatg tgaactggta tcattttgat aatgacagtg atcactgggc tgattttgct
      181 aactggcaca actacaacaa caagtgggat gctgttaatg ctgaattagg tcaattcgta
      241 gacttcagcg ctaacaagaa aatgcgtttc cacggcggtg ttcaatacgc tcgcattgaa
      301 gctgatgtga accgttattt caacaacttt gcctttaacg gattcaactc taagttcaat
      361 ggctttggtc ctcgcactgg tttagacatg aactacgtat ttggcaatgg ctttggtgtt
      421 tatgctaaag gcgctgctgc tattctggtt ggtaccagcg atttctacga tggaatcaac
      481 ttcattactg gttctaaaaa tgctatcgtt cctgagttag aagctaagct tggtgctgat
      541 tacacttacg caatggctca aggcgatttg actttagacg ttggttacat gtggtttaac
      601 tacttcaacg ctatgcacaa tactggcgta tttaatggat ttgaaactga tttcgcagct
      661 tctggtcctt acattggctt gaagtatgt
//
LOCUS       DATNSN010000368          685 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.374, whole genome shotgun sequence.
ACCESSION   DATNSN010000368 DATNSN010000000
VERSION     DATNSN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.374"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>685
                     /locus_tag="VAH76_003233"
     CDS             <1..>685
                     /locus_tag="VAH76_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO1387795.1"
                     /translation="QIIFRNTKTQTFSLTKFYLNRMRRILPALMLVLLVSSVMSYFLL
                     LVPDLKNYSTSLLATILSSSNIYFWKFINIGYFSTDATIQQLLHTWSLGVEEQFYLIW
                     PIILIVLTKKFTDKKIILGTILLTIISFFAYFLLRKHPLLAYYSPITRAFELLTGALV
                     ALSFDNIPAVSNKYLKNLGSILGLFLIIYPACAFTNNDFPSIKILLPCLGTALLIYTG
                     KNNQGFASK"
BASE COUNT          216 a          131 c           90 g          248 t
ORIGIN      
        1 cacagataat atttagaaac acaaaaaccc aaacattctc attaaccaaa ttttacctaa
       61 accgaatgcg aagaattctt cctgcattaa tgctagtgct tctagtttcc tctgttatgt
      121 cctattttct tttattagtc ccagatctaa aaaattacag tacatcactt ctggcaacca
      181 ttctctcaag ctcaaatatc tatttttgga aattcattaa tattggatat ttctcaacag
      241 atgctaccat tcaacagtta cttcatactt ggtctctggg tgtagaggaa caattctatt
      301 tgatatggcc catcatacta attgtactaa ctaaaaaatt tactgataaa aaaattatat
      361 taggcacaat tttgttaaca attatctcgt tttttgccta ttttttgtta aggaaacatc
      421 ctcttttggc gtattattca ccaattactc gagcattcga attattgaca ggcgcattag
      481 tggcattgag tttcgataat attcctgcag taagcaataa atatttaaaa aatctgggtt
      541 ccattttagg cctttttttg attatctacc ctgcttgtgc cttcactaat aatgactttc
      601 ctagcattaa aatccttttg ccttgtttag gtacagctct tttaatttac acgggaaaaa
      661 ataaccaagg atttgcaagc aaaat
//
LOCUS       DATNSN010000369          682 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.375, whole genome shotgun sequence.
ACCESSION   DATNSN010000369 DATNSN010000000
VERSION     DATNSN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.375"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>682
                     /locus_tag="VAH76_003234"
     CDS             <1..>682
                     /locus_tag="VAH76_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEO1387796.1"
                     /translation="QSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYISLFTGEEQL
                     INSQFLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIVAFYFLS
                     LSEQRLFDIWEVNKIIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYNFIRY
                     FIFALLSGCAILNINGFYTLAMQLTLSGIFTFFILYITILIGQSINKFYASLSYSQGK
                     LNVIRIFG"
BASE COUNT          183 a          108 c          105 g          286 t
ORIGIN      
        1 ggcaatccaa tgttatacgc cgttatgtga aattaaaaaa aattcgctgg tctcatatta
       61 cacttttggc tttgtttatg atgacgggtt cattttatgg ctacatttcc ctatttactg
      121 gtgaagagca gctaatcaat tctcaattcc ttttaataaa ggagcttttt tggtttcttt
      181 gcggcacatt attcattgtt tgtttattca aagtcaaaaa aatcaaagct ttttttgatt
      241 ggtatgctat ggatagtgaa ttttttgaaa tactaagcat tgtgattgtt gcattttatt
      301 tcctctcatt atcagaacag agactatttg atatttggga agtcaataag attattgtgg
      361 agttaactcg ctccatcttt ctttttttat tgcttggtac taccgcttat tttactcact
      421 atttctgccg ttctcacctg catatttctt ttgtaaaaag acattataat tttattcggt
      481 actttatttt tgcactgctt tcagggtgcg caattcttaa tattaatggc ttttatactt
      541 tagccatgca gcttactcta tctggcattt ttaccttttt tatcttgtat atcactattt
      601 taataggaca gagtattaat aaattttatg cttcattaag ttactcacaa ggcaaattga
      661 atgttatacg aatattcgga ta
//
LOCUS       DATNSN010000370          659 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.377, whole genome shotgun sequence.
ACCESSION   DATNSN010000370 DATNSN010000000
VERSION     DATNSN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.377"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>659
                     /locus_tag="VAH76_003235"
     CDS             <1..>659
                     /locus_tag="VAH76_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEO1387797.1"
                     /translation="SAKDSIEQTSLLVKERFEIYLNPLNQDLIKITNAINNGIINPDD
                     SKQFDKFLFESIRYNPEIFMVYYGSTTGDFIGVDREQKGIIGLTHVINSVSPPVNVRY
                     QLNEQGEIIGKKLLTRHYDPRVRPWYQQAIKAGKPIWTNVYTFYVFDKSDFLIPGITG
                     ASPIYNKNKQIKGVIALDLTIDGLQHFIRELELTKNTMIYVINNESNIIAFRTPQNKK
                     D"
BASE COUNT          254 a          109 c          103 g          193 t
ORIGIN      
        1 agcgctaaag attcaattga acaaacatca ttactcgtca aagagaggtt tgaaatctat
       61 ttaaatcctt taaatcaaga tttaattaaa ataacaaatg ccataaataa tggcatcatt
      121 aaccctgatg attcaaaaca atttgataaa tttctttttg aatcaattcg atataatcct
      181 gaaatattta tggtttacta tggttcaaca actggtgatt ttataggagt agatagagag
      241 caaaagggaa taataggatt aacccatgtg attaattccg tatcacctcc agtcaacgtt
      301 cgttatcaat taaacgaaca aggagaaata atcggaaaaa aattgctgac ccgccattat
      361 gatccgagag ttcgcccgtg gtatcaacaa gctataaaag caggtaaacc gatatggaca
      421 aacgtatata cattttatgt atttgataaa agtgacttcc ttatccctgg aattaccggg
      481 gcctcaccaa tatataataa aaataagcaa ataaaaggag tgattgctct tgacctgact
      541 attgatggtt tacaacattt tatccgagaa ctggaattaa ctaaaaacac tatgatttat
      601 gtgattaata atgagtccaa tatcattgct ttcagaaccc cacaaaataa aaaagacat
//
LOCUS       DATNSN010000371          649 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.378, whole genome shotgun sequence.
ACCESSION   DATNSN010000371 DATNSN010000000
VERSION     DATNSN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.378"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..184)
                     /locus_tag="VAH76_003236"
     CDS             complement(<1..184)
                     /locus_tag="VAH76_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387798.1"
                     /translation="MWYDTLLEYLGETICPSNKEKARLFISHLFKLLKENSDFGENFE
                     PEFIFGVMVALYNYSIS"
     gene            368..457
                     /locus_tag="VAH76_003237"
     tRNA            368..457
                     /locus_tag="VAH76_003237"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:402..404,aa:Ser,seq:gct)
     gene            464..540
                     /locus_tag="VAH76_003238"
     tRNA            464..540
                     /locus_tag="VAH76_003238"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:498..500,aa:Arg,seq:acg)
BASE COUNT          193 a          128 c          118 g          210 t
ORIGIN      
        1 ggcttattga atagttataa agagcgacca tcacaccaaa aataaactct ggttcaaaat
       61 tttctccaaa atcagagttt tcttttaata atttgaataa atggcttata aataacctgg
      121 ctttttcttt gtttgatggg caaattgttt cacctaaata ttccaataga gtgtcatacc
      181 acataataat cctttaaatc cctttatata ggtagtgtca taacataaat acattttaaa
      241 aaaaatctgc tgatattttt atatgcatta atgatattta ttatgctcaa ttttgaattt
      301 catcacttta tgcaataaga attattgacg catgttaaat acctgatata ctccgctacc
      361 attgattgga gagatggccg agtggtcgaa ggcgctcccc tgctaaggga gtatgggaga
      421 aatcccatcg agggttcgaa tccctctctc tccgccaatc aacgcgcccg tagctcagtt
      481 ggatagagta tctggctacg aaccagaggg tcggaggttc gaatccttcc gggcgcgcca
      541 tttttaatca atttcatacg agcctattca gccgagaata ttcttgacaa tacgttctgt
      601 tacttcatcc gggagtaatt tttgaacttc acgcgtgaac ctgaggttg
//
LOCUS       DATNSN010000372          642 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.379, whole genome shotgun sequence.
ACCESSION   DATNSN010000372 DATNSN010000000
VERSION     DATNSN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.379"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(93..623)
                     /locus_tag="VAH76_003239"
     CDS             complement(93..623)
                     /locus_tag="VAH76_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO1387799.1"
                     /translation="MITIRLAEMNEIQFLNQLISCSARELSQEDYTKEEIEGAIQYVF
                     GVDLELILDKTYFVIEKDGRVAGCGGWSRRKTLFGGSQYKGREQGVYLDPQQDFAKIR
                     AFFINPKFARQGLGSILLKHCEHEALLQKFTRFEMMATLPGVKLYSTFGYHPISNEVI
                     TLPNNVPLRFVRMTKG"
BASE COUNT          206 a          135 c           89 g          212 t
ORIGIN      
        1 ttaagataac cgttcctaac attatgacga taaattaagt ttcaataacc atgagtcaat
       61 aatgactcca gacaatataa aatgaattat tattaacctt tcgtcatccg aacaaatctt
      121 aacggaacat tattggggag agtaatcact tcatttgaga tagggtgata accaaaagtg
      181 ctatagagct taactcccgg taaagtggcc atcatctcaa accgagtaaa tttttgtaaa
      241 agagcctcat gttcacaatg ttttaacaga atacttccca atccttgtct tgcgaatttg
      301 ggattaataa aaaaagctct tatttttgca aaatcttgtt gaggatccag atatactcct
      361 tgttccctac ctttatattg gctgccaccg aataatgttt ttcttcgact ccatcctccg
      421 caaccagcaa ctcgcccatc cttttcgata acaaaatacg ttttgtctaa aattaactct
      481 aaatctacac caaatacata ttgaatggct ccttcaattt cttcctttgt ataatcttcc
      541 tggctaagct ctcttgctga acaagaaatt aattgattta aaaattgaat ctcgttcatt
      601 tcagctaatc tgattgtaat cattggtaac ctcctgcaag tc
//
LOCUS       DATNSN010000373          625 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.380, whole genome shotgun sequence.
ACCESSION   DATNSN010000373 DATNSN010000000
VERSION     DATNSN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.380"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>625
                     /locus_tag="VAH76_003240"
     CDS             <1..>625
                     /locus_tag="VAH76_003240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEO1387800.1"
                     /translation="QLGRWIGESQARRAQTANKLHLTSLPSKNNLLTVVKQHPELKTD
                     SATISAFQAKVRQTLEQYKPGFNVNVGYGFRQGRELDGRKRSDFLSATINMDLPVFPK
                     NRQDRSLKASEDNLLASEKERMSHFRNLREYLNIQYSIWQQQQKSTQLYTTTLIPQAN
                     QYATATMTAYQNFQTDFPTLARAYVRQLNTELEGLKAKVDRDAARINL"
BASE COUNT          216 a          134 c          119 g          156 t
ORIGIN      
        1 tcaattagga cgttggatag gtgaatctca agctcgtaga gcacagactg ctaacaaact
       61 gcacttgact tccttaccat ctaagaacaa cttattgaca gtagtaaaac aacatcctga
      121 gttaaaaacc gattcagcaa ccatctccgc ttttcaagca aaggtgcgac agacacttga
      181 acaatacaaa cctggattta atgtcaatgt cggctatgga tttcgtcagg gccgtgagtt
      241 agatggcagg aaacgatcgg actttttaag tgccaccata aatatggatt tgcctgtatt
      301 tccaaaaaat cggcaggacc gaagcctaaa ggccagtgag gacaacttgc ttgcttccga
      361 gaaagagcgt atgagtcatt ttcgaaattt aagagaatac ctcaatattc aatatagtat
      421 ctggcagcaa caacaaaaaa gcacacagtt atatacaacg acattaattc ctcaagccaa
      481 tcaatatgcc acagccacca tgactgctta tcaaaatttc caaaccgact ttccaacatt
      541 agcaagagct tatgttcgtc agttgaatac tgaattagaa ggattaaaag caaaggtaga
      601 tagggatgcc gctcgtatca atcta
//
LOCUS       DATNSN010000374          583 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.381, whole genome shotgun sequence.
ACCESSION   DATNSN010000374 DATNSN010000000
VERSION     DATNSN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.381"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>583
                     /locus_tag="VAH76_003241"
     CDS             <1..>583
                     /locus_tag="VAH76_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869966.1"
                     /note="type IV secretion system ATPase VirB4 family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HEO1387801.1"
                     /translation="VVDDGVIIGKNGSFMAAWLYRGEDNANTTDEAREMVSFRINQAL
                     SDMGSGWMVHVDVIRRAAPGYSEKGRSHFPDAISMAVDEERRQLFESIGTLYEGYFVI
                     TLTWYPPVLAQKRFVELMFDDSQERLSQKAKTHQLIEEFKQSCRNFESRMSSALDLER
                     LQSEQCIDDEGQTVTQDNFLRHLQFCVTGLNHPV"
BASE COUNT          162 a          103 c          149 g          169 t
ORIGIN      
        1 gttgtcgatg atggcgtgat tataggcaaa aacggttctt ttatggcggc ttggctttat
       61 cgaggcgaag ataatgccaa taccaccgat gaagctcggg agatggtgtc gtttcgcatt
      121 aatcaggcat tgtctgacat gggcagtggt tggatggtgc atgttgatgt cattagacgt
      181 gctgcacctg gttacagtga gaaaggaaga tctcacttcc ccgatgccat ctcaatggct
      241 gttgatgaag aaagaaggca gctatttgaa agtattggga cattatatga gggctatttt
      301 gtcattacct tgacttggta tccaccggta ttggcgcaaa aacgttttgt tgaattgatg
      361 tttgatgata gtcaagagcg tttaagccag aaagcaaaaa cgcatcagct gattgaggaa
      421 tttaagcaat cctgtcgaaa ctttgaatca cgcatgagtt cagcccttga tcttgaaagg
      481 ctacagtctg aacagtgtat tgatgatgaa ggacaaacgg tcacacaaga caacttttta
      541 agacatctgc aattttgtgt taccggtctg aatcatccgg tta
//
LOCUS       DATNSN010000375          562 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.382, whole genome shotgun sequence.
ACCESSION   DATNSN010000375 DATNSN010000000
VERSION     DATNSN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.382"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            5..>562
                     /gene="pieA"
                     /locus_tag="VAH76_003242"
     CDS             5..>562
                     /gene="pieA"
                     /locus_tag="VAH76_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieA/LirC"
                     /protein_id="HEO1387802.1"
                     /translation="MQEKIINLGKGLKKVNEGIKKHISRAEEFLYLANQWEKENVKIN
                     DLLDGVQFSVNDKKMSGQEIKEELSNLIERYNNFLKKNLKGERSETLELKEIQQQLIN
                     FYNAVNDIYTYLSINKTEFFLEAIKSCYKKDQRMSSTIQFVLKIADHLSKERLQFNLN
                     LNSQFAEASESFQEVTTKFYREVATP"
BASE COUNT          226 a           68 c          104 g          164 t
ORIGIN      
        1 tgtaatgcaa gaaaaaatta tcaacttagg gaaggggctt aaaaaggtca atgaaggtat
       61 taaaaaacat atttctcgag cagaagagtt cctctacttg gcgaaccaat gggaaaaaga
      121 gaatgttaag attaatgatt tattggatgg agtgcagttt tcagttaatg ataaaaaaat
      181 gtcgggtcaa gaaattaaag aagaactatc taatctaata gaaagatata ataatttcct
      241 aaaaaaaaac ttaaaagggg aaagaagtga aacattagaa ctaaaggaga ttcagcaaca
      301 acttatcaat ttttataatg ctgttaatga tatttatact tatttatcca taaataaaac
      361 agagtttttt cttgaagcaa tcaagtcttg ctacaaaaaa gatcagagga tgagtagtac
      421 aattcagttt gtgctgaaga tagcggatca tctttctaag gagagactac aatttaattt
      481 gaatttaaat tcgcaatttg cagaagcttc agagagtttt caagaggtaa ctaccaaatt
      541 ttacagagaa gttgctactc ct
//
LOCUS       DATNSN010000376          540 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.384, whole genome shotgun sequence.
ACCESSION   DATNSN010000376 DATNSN010000000
VERSION     DATNSN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.384"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>540
                     /locus_tag="VAH76_003243"
     CDS             <1..>540
                     /locus_tag="VAH76_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387803.1"
                     /translation="FDLSDKFKKESIKKMYKKLSYFIAKQDFKNAVIAFNVLSSLLKL
                     GLLEEQNPSATLTHLVPAEFIANPNTSYFFAFLYEMLNHQRKALVLDKNSISVENVKK
                     LVLITCFLENIEFGLSIHSPLIPNRKSTLSTKLKQELAVIDDFFLNLREMTKSPTFTC
                     ERFLPQWDSFYVESFGDLF"
BASE COUNT          196 a           81 c           82 g          181 t
ORIGIN      
        1 ttttgattta tcagacaaat ttaaaaaaga atcaataaaa aaaatgtata aaaaattgtc
       61 ctatttcata gcgaaacagg atttcaaaaa tgcggttatt gcatttaatg tattaagtag
      121 tttattaaaa cttggtttat tagaagagca aaacccaagt gcaacgctaa cccatcttgt
      181 accggctgaa tttattgcaa accctaacac atcctatttt tttgcatttc tttatgaaat
      241 gctaaatcat cagagaaagg ctcttgtttt agataagaat tccataagcg tagaaaatgt
      301 aaaaaaatta gtgcttataa cctgtttttt agaaaatatt gaatttggcc ttagtattca
      361 ctcaccatta attcctaata gaaaatcgac tttatcgaca aagcttaaac aagagctggc
      421 agtaatagat gatttttttc tgaatttaag agagatgaca aaaagcccta catttacctg
      481 cgaaagattt ctgcctcaat gggatagttt ttatgtagaa tcctttggcg atctatttga
//
LOCUS       DATNSN010000377          532 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.385, whole genome shotgun sequence.
ACCESSION   DATNSN010000377 DATNSN010000000
VERSION     DATNSN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.385"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>532
                     /locus_tag="VAH76_003244"
     CDS             <1..>532
                     /locus_tag="VAH76_003244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387804.1"
                     /translation="YYQSIPFNHKKYPSKSGFIQPGDIDNLPRIIENWFNFYDSYSPV
                     VHLYFSFLTNTHSFAETKFISLSQALEAYHRLSGYNQDAMAAEEFEELKSNALTSCTT
                     TQKEWLNNQFTYLNKINLRKRLKELINPIKQYYGNKAAREMLIDNIMDTRNYLTHITS
                     ENMKKLHEKNNLTELCL"
BASE COUNT          201 a           92 c           79 g          160 t
ORIGIN      
        1 tattaccaat ctattccatt taaccataaa aaatatccat caaaaagtgg cttcatacaa
       61 cctggggaca ttgacaatct tccaaggatt attgaaaact ggtttaattt ttatgactcc
      121 tattcccctg tagtacactt atatttttca tttttgacaa acacccactc cttcgctgaa
      181 acaaaattca tatctctgtc tcaggcatta gaagcttatc atagattaag tggctataat
      241 caggatgcta tggctgctga agaattcgag gaattaaagt caaacgcttt aacttcttgt
      301 actacaactc aaaaagaatg gttaaataac cagtttacct acctaaataa aataaatttg
      361 agaaagcggc tgaaggaatt aattaatcca attaagcaat attatggtaa caaagcggca
      421 agagaaatgt tgatagataa tattatggat acaagaaatt atctgactca tattactagt
      481 gaaaatatga agaaactgca tgaaaaaaac aacttaactg agctgtgcct ta
//
LOCUS       DATNSN010000378          523 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.386, whole genome shotgun sequence.
ACCESSION   DATNSN010000378 DATNSN010000000
VERSION     DATNSN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.386"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            164..376
                     /locus_tag="VAH76_003245"
     CDS             164..376
                     /locus_tag="VAH76_003245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387805.1"
                     /translation="MKTLSETNPYLHDKENAKKLNARSTRTSCGVEGIVKHDLPPIEI
                     DHSRSDKVLKEMKQRLQGSSEKSSDS"
BASE COUNT          188 a           81 c           84 g          170 t
ORIGIN      
        1 tagagtgaac aaatatgggt tatttgtccc acctgtacca ttgagaattt tgatggtggt
       61 atgtctttaa cccagtatta taaagtgtcc cattagttcc acttgtacca ctgaaaatta
      121 ttatgtttgc tcccattaca cgaaaaggga gtataatatt aatatgaaaa cattgtctga
      181 aactaatcct tatcttcatg ataaagaaaa tgctaaaaaa ctaaatgccc gctcgaccag
      241 aacatcttgt ggagttgaag gtattgttaa gcatgattta ccaccaatcg aaatagatca
      301 ttctagatct gataaggtat taaaagaaat gaaacaaaga ctccaaggat cctctgaaaa
      361 atcaagtgac tcttaattat tatctcttcg ttatgtatta aatgtggaat atttgaacaa
      421 atatttgcaa ttgtagtaat atttaactac aatttaatta aatctaatta cagagttatc
      481 gttatgtcta caaaaactgt acaggctaga gtggatagcc att
//
LOCUS       DATNSN010000379          486 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.389, whole genome shotgun sequence.
ACCESSION   DATNSN010000379 DATNSN010000000
VERSION     DATNSN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.389"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>486
                     /locus_tag="VAH76_003246"
     CDS             <1..>486
                     /locus_tag="VAH76_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEO1387806.1"
                     /translation="EVLFTALMTPLTNKSLMDTSHVPAPKKLYRGLNLPQEFTNKLIN
                     QANAIIANTENTLFTDLSAEAFKQIKLNDFSQMSGRTCASTTKNMKLLTDIWGSNVIF
                     EMLDPDGLLHPKQVGTHMAGSEDEFSVYLPEDVALVPTKVTLDGKTDTGEDRYIFTLV
                     AV"
BASE COUNT          160 a          102 c           87 g          137 t
ORIGIN      
        1 gaagtgctgt ttaccgcctt aatgacgcct ttgactaata aatcattaat ggatacatcc
       61 cacgtcccag ctccaaaaaa actgtatcgt ggattaaatc tgccacaaga atttaccaat
      121 aaattaatca atcaagccaa tgccattatt gccaatacag aaaatacgct ttttactgat
      181 ctctcagctg aagccttcaa acaaatcaag ttaaatgatt ttagccaaat gtctggcaga
      241 acctgtgcca gtacaaccaa aaatatgaaa cttttaaccg atatatgggg ctccaatgtc
      301 atttttgaaa tgcttgaccc ggatggttta ctgcatccaa aacaagtagg aactcatatg
      361 gctgggtctg aagatgaatt ttccgtttat ttgccggaag atgtcgcttt agttccaacc
      421 aaggtaactc ttgatggaaa aacagacaca ggagaagatc ggtatatctt tacactggtt
      481 gccgta
//
LOCUS       DATNSN010000380          485 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.390, whole genome shotgun sequence.
ACCESSION   DATNSN010000380 DATNSN010000000
VERSION     DATNSN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.390"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>485)
                     /locus_tag="VAH76_003247"
     CDS             complement(<1..>485)
                     /locus_tag="VAH76_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEO1387807.1"
                     /translation="IDLKEYLHKLKLGFQLLSDLLPEEQIKYLNSIYLAHIKVFPYSN
                     FELRRIANQHPVQRQSLSFFNYAKLLSSEHGGYCYQSAGLLFDALVQLGFKTEYCAAR
                     VLVGEPVNSPKVLAIPATHLVLVVTLGDKKFLLDPGLGASAPRLPILITEKDESILQG
                     K"
BASE COUNT          162 a           94 c           90 g          139 t
ORIGIN      
        1 ctttaccttg taaaatggac tcgtcttttt ctgttatcaa tattgggagc cttggagcag
       61 aagcgcccaa tcccggatcc agaagaaatt ttttgtctcc cagagtcacg actaaaacaa
      121 gatgagtagc aggtatcgca agtaccttag gggagttcac aggttcgcca acaagaacac
      181 gcgccgcaca atattccgtc ttaaatccca attgcactaa tgcatcaaac aacagacccg
      241 cactttgata acaatacccc ccatgctctg aagaaagcag ctttgcatag ttaaaaaaag
      301 ataacgattg tctttggaca ggatgttgat tagcaattct gcgtaattca aaattggaat
      361 aaggaaaaac tttaatatgt gctaaataaa tactgtttaa gtatttaatc tgttcttcgg
      421 ggagtaaatc agataataat tgaaaaccta attttaattt atggagatat tcctttaagt
      481 cgatg
//
LOCUS       DATNSN010000381          481 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.391, whole genome shotgun sequence.
ACCESSION   DATNSN010000381 DATNSN010000000
VERSION     DATNSN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.391"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>481
                     /locus_tag="VAH76_003248"
     CDS             <1..>481
                     /locus_tag="VAH76_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEO1387808.1"
                     /translation="RMESANKLLELMNNFVDQYISQGASPEDVIKKLKPFEKYFVIEI
                     SHYEEFGHLVYKTNFNCNPALFDVNPIEFLNELFVQFYKDTTLSSRSKAALKKVLDKF
                     EITYEVPKTWYSYIDWDIPIVTDKNSVLVTYDKQAYNKYHQESGQREMEFFETIERCL
                     "
BASE COUNT          176 a           62 c           86 g          157 t
ORIGIN      
        1 agaatggagt ctgcaaacaa attgttagag ctgatgaata atttcgtaga ccaatatata
       61 tcacaagggg caagtccaga agatgtaata aaaaaactta agccatttga gaaatatttt
      121 gttattgaaa tttcgcatta tgaagagttt gggcatttag tatataaaac aaactttaat
      181 tgtaatccag cgttatttga tgttaatcct atcgaatttt taaatgaatt attcgtgcaa
      241 ttttataaag acaccacttt atcatctcgc tctaaagctg ctttaaaaaa agtgcttgat
      301 aaatttgaga ttacttatga ggtgccaaag acctggtata gttatattga ttgggatatt
      361 cctattgtaa ctgataaaaa ttcagtatta gttacttatg acaaacaagc ttataacaaa
      421 tatcatcaag aaagcggcca gagagaaatg gagtttttcg agacaattga acgatgtttg
      481 g
//
LOCUS       DATNSN010000382          478 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.393, whole genome shotgun sequence.
ACCESSION   DATNSN010000382 DATNSN010000000
VERSION     DATNSN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.393"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          162 a           67 c           67 g          182 t
ORIGIN      
        1 tagtagatat atagatttta tgaaagcagt atattattaa gaaagtataa aatattaact
       61 aagtaaattc attaattatt aattttatat tccgtttata tatttgtttt ttgcttttca
      121 agtaaattga tgtgccaatc atgctcaaac caagaaaacc ttccctcaaa atccattccc
      181 ttaataaaaa tttaggcttg cttatgtcga gctgaggtta attaaccttt ggtgttgctt
      241 gataacccaa tttatggttg gttgagtggt cataaaaaaa ttgttctacc atcatttact
      301 ttaatctcca gtaataggta agtattagta tttaatggag attaaagtaa atatcatgtg
      361 gtgttaccag ccctcaatac tttaaaaaat tcaaataaca accctctgtt ttccatggct
      421 attaattaaa acatggtgct gttttgttgg tttaataata aaaacaaccg tctgtttt
//
LOCUS       DATNSN010000383          471 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.394, whole genome shotgun sequence.
ACCESSION   DATNSN010000383 DATNSN010000000
VERSION     DATNSN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.394"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            4..279
                     /locus_tag="VAH76_003249"
     CDS             4..279
                     /locus_tag="VAH76_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387809.1"
                     /translation="MKEKHFAYKITLSSTGLVCTHSEIEENKMIDLLKEIKSDDSQAI
                     FWIFKQFDKQPKEQLGIIDCSKNRIYYHYSGEVEEIDEAIEKLSKKI"
BASE COUNT          197 a           73 c           69 g          132 t
ORIGIN      
        1 atcatgaaag aaaaacactt cgcctataaa attactcttt cttcaacagg cctggtttgt
       61 acacattctg aaattgaaga aaacaagatg attgatctgt taaaagaaat aaaaagcgat
      121 gattcccaag ccattttctg gatctttaag cagtttgata agcagcctaa agaacaatta
      181 gggatcatcg attgcagcaa aaaccgtatt tattaccatt attctggtga agttgaagaa
      241 attgatgaag ctattgaaaa actaagtaaa aaaatataaa tccaaaagta gctacaatag
      301 acagatttat tttcatcgaa tgcaacaata tttaaacgca tggattgaaa atatagtgca
      361 caaagtcata cgaaaacaaa cacaccataa tatgaaaatt aagaccaaat aaatccaatt
      421 aaatactttt agtaaatttt tagttctatc aaccactgga caaaagtgtg t
//
LOCUS       DATNSN010000384          470 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.396, whole genome shotgun sequence.
ACCESSION   DATNSN010000384 DATNSN010000000
VERSION     DATNSN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.396"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>470
                     /locus_tag="VAH76_003250"
     CDS             <1..>470
                     /locus_tag="VAH76_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387810.1"
                     /translation="SQITDDGLKKEIESRIIDYFSKLSLPEHVTIYDQLLLSLREKDA
                     IFTFNWDPFLFDAYKRNSDIVSLPQIFFLHGNVRIGACEACDKWGEKNTYCPECDIRF
                     KDVPLLYPIKNKSYFQSSKYTALSWKKAESWFSEAFTITIFGYSAPTSDIEAVS"
BASE COUNT          160 a           70 c           88 g          152 t
ORIGIN      
        1 tagtcaaatc actgatgatg gtttaaaaaa agaaatagag agtaggataa tagattattt
       61 ctcaaagctt tcattaccag aacatgtaac tatttacgat caattgttat tatcacttcg
      121 agaaaaagac gccattttca cattcaactg ggatccattt ttatttgacg catataaaag
      181 aaatagtgat attgtttcat tgcctcagat atttttcttg catggtaatg taagaatagg
      241 cgcttgtgaa gcatgcgata aatggggaga aaaaaatacg tattgtcctg agtgtgatat
      301 tagattcaaa gatgtacctc ttctttatcc aattaaaaat aagagttact ttcagtcgag
      361 taaatatact gccttaagtt ggaaaaaagc ggagagttgg ttctctgagg catttactat
      421 aaccatattt ggatatagtg ctccaacctc agatatcgag gccgttagtc
//
LOCUS       DATNSN010000385          453 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.397, whole genome shotgun sequence.
ACCESSION   DATNSN010000385 DATNSN010000000
VERSION     DATNSN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.397"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..453
                     /locus_tag="VAH76_003251"
     CDS             <1..453
                     /locus_tag="VAH76_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387811.1"
                     /translation="LTYLQQLNDTELYRLSLIVDESDYKNLQVNSKEDFARDIIRRIF
                     NYHQLFKQNDLIGYLLKTDGSTNENQRVQHYDIQIEIYISDIKPIFALIEKGITKNAI
                     SDRPVNNIGGKLCCRLCETGLNVLHAQFYSDQVAFDNDKFPQLSTFAP"
BASE COUNT          157 a           83 c           77 g          136 t
ORIGIN      
        1 ctgacatact tacagcagct aaacgataca gagctctatc gactttcatt aattgttgat
       61 gagagcgatt ataagaattt acaagtcaat tcaaaagaag attttgcccg agacattatt
      121 cgtcgaatat ttaattatca tcaactcttc aaacagaatg acttgatagg ctatttgtta
      181 aaaaccgatg gctcaaccaa tgaaaatcaa agagtgcagc attacgatat tcaaattgaa
      241 atctatatta gtgacattaa acctatattc gctctgatag agaaaggaat tactaagaat
      301 gcaatatccg atagaccagt taacaatatt ggtggaaaat tgtgctgtcg cctttgcgag
      361 actggcttga atgtgttgca cgcccagttc tatagtgatc aagtagcttt tgacaatgat
      421 aaatttccac aactttcaac attcgctccc taa
//
LOCUS       DATNSN010000386          453 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.398, whole genome shotgun sequence.
ACCESSION   DATNSN010000386 DATNSN010000000
VERSION     DATNSN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.398"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          158 a           68 c           79 g          148 t
ORIGIN      
        1 tttgccttga ccttggcgtt tttcgtaagt cctgttagaa agacaaaacg aaaaataaaa
       61 accatttgct tttagtgatt ataaattcgt atattttaat gaaaaagttc actcctgttg
      121 aaactgaggt aatttcatct caaagttatc tttagtaata tctgcgccaa caatgcgaaa
      181 tgatctaaag gatgaagata tgtcttaaat aagtcaatga tctcatgtaa aaattttgaa
      241 tttacaaagt caaagtgact cttttgtaag cgatgtagct accatggaga agaatcaagg
      301 tctaaattaa atggtatgcg aactggtcga aatttcgttg ccagtagatg gatatattct
      361 cttttatttt aaatccacaa caaaaagtcg ggtcaataag acccgacttc agaatttcat
      421 gcgatttggg ttaaggtcaa ttaaaatcct ata
//
LOCUS       DATNSN010000387          417 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.401, whole genome shotgun sequence.
ACCESSION   DATNSN010000387 DATNSN010000000
VERSION     DATNSN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.401"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>417
                     /locus_tag="VAH76_003252"
     CDS             <1..>417
                     /locus_tag="VAH76_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEO1387812.1"
                     /translation="KRASQNKVFKGLAEKSKSTMGWYFGFKLHLIVNDKGELMAFKIT
                     SSRTDDRFVVPNLSENLFGKMIGDKGYISQNLTDKLAERGLQLLTKARKNMKQKVLNT
                     FDKALLRKRAIVESVIDQLKNISNIEHSRHRSIFNF"
BASE COUNT          151 a           66 c           79 g          121 t
ORIGIN      
        1 taaaaagagc aagccagaac aaggtcttta agggtttggc agagaagtcc aagtccacaa
       61 tgggatggta tttcggcttt aagcttcatt tgatagtcaa tgacaaaggc gaattaatgg
      121 cttttaaaat aacatcaagc cgaactgatg accgatttgt cgttcctaat ttgagcgaaa
      181 atctgtttgg taaaatgatt ggcgataaag gttatatttc tcaaaattta actgacaaat
      241 tggctgagcg aggattacaa ctccttacta aagccaggaa aaacatgaaa caaaaagtac
      301 tcaatacatt tgataaagct ttgcttagaa aaagagccat tgttgagtca gttatcgatc
      361 aattgaaaaa tatttccaat attgaacatt caaggcatcg ctctatattc aatttta
//
LOCUS       DATNSN010000388          404 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.402, whole genome shotgun sequence.
ACCESSION   DATNSN010000388 DATNSN010000000
VERSION     DATNSN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.402"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          148 a           73 c           62 g          121 t
ORIGIN      
        1 aatactatat acagtaatat ggctattgat ttgtacactt tgcttgtgta acaaaggaac
       61 tgtattgaat atatgaaagg aactcaaaac atgagcttaa aattaaatga tgtaatcgag
      121 aaaacccttg aagccgtaaa aaaaacacca aaccgggagt aagtacctcc gtcgaatttg
      181 atctcattat atctgattca acaaaagatg gtctgttgat tacacccacc cttggacaac
      241 atcatgatgg gtctagattg aaattcacga taagcacccc ggtaaataaa taagcactat
      301 gacttaaaca agattttgtg actatcttgt gctacaaagc acaagagtct acttttttaa
      361 tatatttcat tccatgccaa tatgatcaat tttttacact cata
//
LOCUS       DATNSN010000389          401 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.404, whole genome shotgun sequence.
ACCESSION   DATNSN010000389 DATNSN010000000
VERSION     DATNSN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.404"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..221)
                     /locus_tag="VAH76_003253"
     CDS             complement(<1..221)
                     /locus_tag="VAH76_003253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387813.1"
                     /translation="MGSTSATQAGVNAGNTVFDLSVDGAGVVTLRQYAEIDHPIADDP
                     SASGAPFDDQLAVLGNNLVSLTATALTTDG"
BASE COUNT           97 a          125 c           94 g           85 t
ORIGIN      
        1 ccatcggttg tcaacgccgt cgctgtcaaa gacaccaaat tgttgcctaa aacagccagt
       61 tgatcgtcaa aaggagcacc gctggcactt gggtcgtcgg ctatcggatg gtcgatttca
      121 gcgtattgcc ttaaggtcac aacccctgcg ccatccacac tcaaatcaaa aacagtattg
      181 ccagcattca ctccagcctg cgtggccgac gtcgaaccca cgatcgtgcc gctgatgtcg
      241 tacaggtaaa tgttgctgcc attgctgtct aatccggaat cagcaccgga agccgcgata
      301 aggcccaaag aataactcat caccgtgctg ccggccccat ccgccccata attcggggtg
      361 ttgctgaaca cgttgccaaa gttcgcactc gtcacagccg c
//
LOCUS       DATNSN010000390          401 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.405, whole genome shotgun sequence.
ACCESSION   DATNSN010000390 DATNSN010000000
VERSION     DATNSN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.405"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>401)
                     /locus_tag="VAH76_003254"
     CDS             complement(<1..>401)
                     /locus_tag="VAH76_003254"
                     /inference="COORDINATES: protein motif:HMM:NF027890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO1387814.1"
                     /translation="ENNIERIHTFTDGWIKEQFVKTTGELVKEGQLLMTLYSPTLNNA
                     QEELLIALKNNNKALIEAGKKKLLTLGMANSQINQLMSSRKLMDRVKIFTTQSGIVSK
                     INVREGQYVKPDTELLSIEDLSQIWIIAEVF"
BASE COUNT          105 a           73 c           64 g          159 t
ORIGIN      
        1 tcaaaaactt cagcgatgat ccaaatttgt gataaatctt caatgcttaa taattcagta
       61 tcaggtttaa catattgtcc ttcacgcaca tttatttttg agacgatacc gctttgggtg
      121 gtaaagattt tcactctgtc catcaatttt ctggatgaca ttaattgatt gatttggcta
      181 tttgccatac ctaaggttaa taactttttc tttcctgcct cgatcaaggc tttgttatta
      241 tttttgaggg caattaataa ttcttcttgc gcgttattta gggttgggga gtatagtgtc
      301 attaacaact gcccctcttt aactaattct cccgttgttt taacaaattg ttcttttatc
      361 caaccatccg taaaggtatg aatgcgttca atattatttt c
//
LOCUS       DATNSN010000391          392 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.406, whole genome shotgun sequence.
ACCESSION   DATNSN010000391 DATNSN010000000
VERSION     DATNSN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.406"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          165 a           55 c           51 g          121 t
ORIGIN      
        1 attacttttt cctttaattg ttcacatgat aaaatacccc gagacattct agatgggatt
       61 agtggtgaat caattagtat atctaaaaaa ctagttaaca ttataaataa aaacggtttt
      121 gagattaatt ataaaaaagt ccgtctgtca agctcaaacc aaagacagca agttacaggt
      181 atagttacaa atgtgaaagt taatttgcct cgaaaatata taaaaaaaac tcaatcaatg
      241 ttatatgcct ggaaaaattt ctccctaccc aatgccgaac aagcacatat caataattat
      301 ctagaaaagc cttataaata tcaaaggttc attagatata ataaagacaa aagttatttt
      361 aatttaatga ttaaaggaag aattaattat at
//
LOCUS       DATNSN010000392          387 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.409, whole genome shotgun sequence.
ACCESSION   DATNSN010000392 DATNSN010000000
VERSION     DATNSN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.409"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>387
                     /locus_tag="VAH76_003255"
     CDS             <1..>387
                     /locus_tag="VAH76_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HEO1387815.1"
                     /translation="QWPIVNKKIMMEHFDDINTVNVKKTEALEIALRAEHTRDFSPMI
                     NNIAVGLSSGTSGSRGLFLVSEQERDAWAGIILAKAMPNGLRSRERIAFFLRANNQLY
                     TTLNKSRTIQFHFFDLFADFDTHIQRL"
BASE COUNT          121 a           63 c           79 g          124 t
ORIGIN      
        1 caatggccaa ttgttaataa aaaaatcatg atggaacact ttgatgatat taatacagta
       61 aatgtcaaaa aaactgaggc attggaaatt gctttacgtg ctgagcatac acgtgatttt
      121 tccccaatga taaataatat tgctgttggt ttatcttcag ggacttcggg gagcagaggc
      181 ttgtttttag taagtgagca ggaacgtgat gcctgggccg gaataattct tgccaaggct
      241 atgccgaacg gtttgcgttc gcgagaacga attgcctttt ttctaagagc aaataaccag
      301 ttgtatacca ctttaaataa aagtagaaca atacaattcc atttttttga tctttttgct
      361 gattttgata ctcatatcca gagatta
//
LOCUS       DATNSN010000393          367 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.410, whole genome shotgun sequence.
ACCESSION   DATNSN010000393 DATNSN010000000
VERSION     DATNSN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.410"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>367)
                     /locus_tag="VAH76_003256"
     CDS             complement(<1..>367)
                     /locus_tag="VAH76_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO1387816.1"
                     /translation="DYSAMEEIIYFQLDDCITKNGEKIFALSHKLPLKDEDGKTHGVI
                     GFTKFMNLHQLMSNLNAKNTFLTPNLKINVNVDDWLKTTINLSRREMEVLYYFLQGKS
                     IKLIAPLLHITERTVIFHLN"
BASE COUNT          125 a           57 c           54 g          131 t
ORIGIN      
        1 tattaagatg aaaaataacg gttctttcag taatatgaag caatggtgca ataagcttga
       61 tcgattttcc ttgtaaaaaa taatacaaaa cttccatttc tctccgagac aaattaatcg
      121 ttgtttttag ccaatcatca acattaacat tgattttaag attaggtgtt aaaaaggtat
      181 tttttgcatt aaggttactc atcagttgat ggagattcat aaatttcgta aagccaatga
      241 caccatgagt ttttccatct tcatctttaa gcggtaattt gtgagacaag gcaaatattt
      301 tttcaccgtt tttagtaata caatcatcca attgaaagta aataatttct tccattgccg
      361 agtaatc
//
LOCUS       DATNSN010000394          361 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.412, whole genome shotgun sequence.
ACCESSION   DATNSN010000394 DATNSN010000000
VERSION     DATNSN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.412"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          156 a           42 c           62 g          101 t
ORIGIN      
        1 tggttacaat catcttaaaa aagaattaag taaaaaaata attgatatca acagaatttt
       61 tcattcgaac gactttaaaa agttaagtca aagtgatcaa aaggaatatg agaggcagat
      121 atatgttgcg tttataagtc acataatagg ttatctccca agtgtaaaac atgaagatta
      181 cagctcagaa aatgaggtaa gattgctgtt aatggatggt aagagaaggg atataaaaaa
      241 ttacccggat gggtgtcaat ttgaatattt tataaatcag agagaattac atatcccaac
      301 aatagactta cagttacaaa ttcaatgtag attgataacc aaacaaaaaa aatatgttaa
      361 c
//
LOCUS       DATNSN010000395          355 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.413, whole genome shotgun sequence.
ACCESSION   DATNSN010000395 DATNSN010000000
VERSION     DATNSN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.413"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>355
                     /locus_tag="VAH76_003257"
     CDS             <1..>355
                     /locus_tag="VAH76_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEO1387817.1"
                     /translation="ANRFQLSPLIIGLTIVALGTSAPELFISVIFSLKDKNDLAIGNA
                     IGSNIANIGLILGIIILIKPTSFHYNKLKKTYPILIIVMLFAYSLILDGYLGTIDGCL
                     FLIGCIAVLCYFIYLA"
BASE COUNT          105 a           63 c           56 g          131 t
ORIGIN      
        1 ggccaatcgt tttcaattat caccacttat cattggttta accattgttg cattgggtac
       61 cagtgccccc gaattgttca tctctgttat tttttcctta aaagataaaa atgatctggc
      121 aattggaaac gccattggat caaacatagc aaatattggc ttaatcttag ggattataat
      181 ccttataaaa ccaacatctt ttcattacaa taaactaaaa aaaacatacc caatattgat
      241 cattgtcatg ctgtttgcat acagtttaat actggatggt tacctgggta caatagatgg
      301 ttgccttttt ttgattggat gtatagctgt actctgttat ttcatttatt tggct
//
LOCUS       DATNSN010000396          353 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.414, whole genome shotgun sequence.
ACCESSION   DATNSN010000396 DATNSN010000000
VERSION     DATNSN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.414"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>353
                     /locus_tag="VAH76_003258"
     CDS             <1..>353
                     /locus_tag="VAH76_003258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387818.1"
                     /translation="DMLYHFTDIRALKNILENRELRFTYFKDLNDSREIYYGLEQIKQ
                     HLNNIDLRNKKLFLDMLESFIEPGNKNVNVFICSFSNSYNHLPLWRFYGDNGRGVAIA
                     FDYNFPLENEDILPK"
BASE COUNT          124 a           39 c           68 g          122 t
ORIGIN      
        1 aagacatgct ttatcacttt acagatatta gagcgctaaa gaatattttg gaaaatcggg
       61 agttgagatt cacctatttt aaagatttaa atgatagtcg tgaaatttat tacggattag
      121 aacaaattaa gcagcatttg aacaatatcg atttgagaaa taaaaagtta tttttggata
      181 tgctggagag ttttatagag ccggggaata aaaacgttaa cgtattcata tgttcattct
      241 caaatagtta taatcatctt cctctgtgga gattttatgg ggataatggt cgaggagtgg
      301 ctatagcatt tgattataat tttcctttag aaaatgaaga tattttacct aaa
//
LOCUS       DATNSN010000397          345 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.denovo.416, whole genome shotgun sequence.
ACCESSION   DATNSN010000397 DATNSN010000000
VERSION     DATNSN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.denovo.416"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>345)
                     /locus_tag="VAH76_003259"
     CDS             complement(<1..>345)
                     /locus_tag="VAH76_003259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387819.1"
                     /translation="FVSFQFSVYTLLSVFIVFVFCFESDLKEKIVKQKIKALDLCKIL
                     GVTQHQKIAELSNITNSKSLAALITIIFNDNSIENYQHSDEKDIKEWKNHLQINVDDS
                     EHYKENFFKRWS"
BASE COUNT          119 a           46 c           47 g          133 t
ORIGIN      
        1 tctgaccaac gtttgaagaa attttcttta taatgctcag agtcatctac atttatttgt
       61 aaatggtttt tccattcctt gatgtccttt tcatcactat gttgataatt ttcaatacta
      121 ttatcattga aaataatagt aattagagct gcaagtgatt tactgttggt gatatttgaa
      181 agttcagcaa ttttttgatg ttgtgtgaca cctaatattt tgcataaatc cagagcttta
      241 attttttgtt ttactatttt ttcttttaaa tcagattcaa agcaaaaaac aaatactata
      301 aaaacagata aaagtgtata gacagaaaac tgaaaggaca caaat
//
LOCUS       DATNSN010000398         5059 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.guided.192, whole genome shotgun sequence.
ACCESSION   DATNSN010000398 DATNSN010000000
VERSION     DATNSN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.guided.192"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..628
                     /gene="ceg23"
                     /locus_tag="VAH76_003260"
     CDS             <1..628
                     /gene="ceg23"
                     /locus_tag="VAH76_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg23"
                     /protein_id="HEO1387820.1"
                     /translation="INQPLTIYDRSTSSVVAEYVNPKVNLPDFEVAIDALQGHYFLLK
                     TEETEKELEEYERSHAQYKKDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFVALIES
                     LSEINSLSQIDTNLKNENTDTPNCNFLLKVGASVVGTSSALITLMGIAILSGQYDPGN
                     SLAEEALATTVVSSIVGLGYGLYSFFSSSPKLSELDSTNGLSQKLKMN"
     gene            820..1239
                     /locus_tag="VAH76_003261"
     CDS             820..1239
                     /locus_tag="VAH76_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEO1387821.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFMPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            1239..3149
                     /locus_tag="VAH76_003262"
     CDS             1239..3149
                     /locus_tag="VAH76_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEO1387822.1"
                     /translation="MLLIEIVLTIHCLLLVSSLFIGRAWLANKPSFKLKLLRFLLASC
                     VISPIIVHCFNFDQKPERQNYVSFDVLQQYVKQPIFKTESSKFSHESSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLAEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREITCTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSMVKE
                     VDISHLDPRMQQIAERQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSYETKNYDCHSPYYIAGKTFTNYSSNVESASLAEA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDTSTWWQADRSDGLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETTNYSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGHFSKNGKTLVLGGELAAPVFR
                     NVAMDALSSVNQ"
     gene            3199..3999
                     /gene="blaOXA"
                     /locus_tag="VAH76_003263"
     CDS             3199..3999
                     /gene="blaOXA"
                     /locus_tag="VAH76_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEO1387823.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4059..4934)
                     /locus_tag="VAH76_003264"
     CDS             complement(4059..4934)
                     /locus_tag="VAH76_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEO1387824.1"
                     /translation="MDKSPKLMHLLIYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNYQKKTYDAFDMNQILEQLMGADLQKNNANQVLLAQ
                     TIDKYVSQTPLLYFTTKGHAYLAYQETPGTISDNIITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGAQGRAPDLGMVMSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINNQ"
BASE COUNT         1569 a          899 c          979 g         1612 t
ORIGIN      
        1 cattaatcag ccacttacaa tatatgatcg ttctacctcc tcagttgtag ccgagtatgt
       61 caacccaaag gtcaatttac ctgattttga agtggcaatt gatgctcttc aagggcatta
      121 ttttctttta aaaactgagg agacggaaaa agagttagaa gaatatgaaa gaagtcatgc
      181 ccagtataaa aaagatagaa gtgaaatcct tgcacactcc gataagcctg tttcttcctt
      241 attggttcga gctacttgcc ccaaaggtca tttggatgaa gatccttttg ttgcattaat
      301 tgaaagcctt tccgaaataa acagccttag ccaaattgat actaatttga aaaacgagaa
      361 tacagatacc cccaattgca acttcctttt aaaggtaggt gcttctgtag ttggtacctc
      421 ttctgcacta ataactttaa tggggatagc catattatct ggccaatatg atcccggaaa
      481 cagtctggct gaagaggcat tagctactac tgttgtatcc agtattgtgg gtttaggtta
      541 tgggctttat agcttcttta gttctagccc taagttatca gagcttgatt ctaccaatgg
      601 attatcgcaa aaacttaaaa tgaactaacc tcctgaatta gaaaatattg tctgtaagcc
      661 gatcgtgttg atattaggta tcaacacgat tgcgccattc aagtaaactg ttctcgcgtg
      721 tagagaagta tttttcccat aactatttca actctactat ttttatagta gaatcctact
      781 ataaaaatag tagagtgtat gtggttattg gagagaaaca tgtcggcaaa aaaaaattcg
      841 atagctcatg atcgacttct tacagaagta gagcttgaat taatgaatat tatctggagc
      901 cttgataagg taactattaa ggaggttgtt tcacatctgc ctaaggaaag gcctttggct
      961 tatacaaccg tggcaacagt actaaaagtg ctggaacaaa agggatttct tgagtgtcaa
     1021 aaaaattcct atgctcatgt ttttatgccc atagttacaa agtcagatta tgaaaacact
     1081 tgtattgatc atatggttgc caatgttttt gatggagagc cccgagcact tgtccaaaga
     1141 cttttattag ccagaaagtt acagcatgat gacattcaag ccattgaaga agcacttaaa
     1201 caattaaatg ctggtgaaaa acagccagag gtgatgtaat gttattaata gaaatcgtat
     1261 tgactattca ttgcctgctt cttgtcagtt cactatttat agggcgtgcc tggttagcaa
     1321 ataaaccatc ttttaaactg aaattactcc gttttttatt ggccagttgc gttatttcac
     1381 caataatagt tcattgtttt aattttgatc aaaagcctga acgacaaaac tatgtatcat
     1441 ttgatgtttt gcaacagtat gtaaagcaac ccatatttaa aacagaatct tcaaaattta
     1501 gtcatgaatc gtcttctgcc tttacatcga tgaatgttaa ctactgtcaa ctcttaagcg
     1561 tgatgttttt tttaatcgtc ttgtttcgta gttatcactt gttatcaggt ttaggtcgct
     1621 tgaaattgat gttggctgaa gcaatcccat atcgaacctc cggaaaacta gttattaagg
     1681 tttctcacca ctgccatgtt cctttctcaa tattgctatt taataaagcc tacatcgtat
     1741 tgccagtatc catattttct tcttccatga atgtgaggat tgctattgct catgaagggc
     1801 aacaccatcg taatggagat tgttcatggg cctattttat cgaaatcata cgtattatct
     1861 tctttggaaa cccgggtgta actcaatggt ttcgtgtttt agcagattta caggaattgt
     1921 catgtgatga agtattggtt ggacatcaaa aaatttcggc acatgactat ggtcactgtc
     1981 tattgcaagt ggtagaggca gtatcccaat gctctctggc atctaaccgg gaaattacat
     2041 gcacggtggg tatggctttg aacaaagaaa atgaagactg caaatttata ataaggagaa
     2101 tttcaatgct aactgcttat tcacccaatg catccaggcc tttgctatta gggatagtct
     2161 ttgcaggatt ttctatcctg tcaccaatct gtgttgccta ttcagcagca ggatctttga
     2221 cgagttccat ggtaaaagaa gttgatattt ctcatttaga tcccagaatg cagcagattg
     2281 cagagagaca aattacgaca gccgtcaaac aatatcatgc aaaatctgga gttattgcaa
     2341 tagctgatcc ccaaacaggc aatatcattg catttgctga atcaagcaaa aataaggggt
     2401 tagagagttg gaaatcacgt attttttcac ccggatctac tataaaacct tttattgcag
     2461 cagctgcaat caactctgga aatagctatg aaacaaaaaa ttatgattgt cattcacctt
     2521 attacatagc aggcaaaaca tttacaaatt atagttctaa tgttgaatca gcttccttag
     2581 cagaagctat cgcaaaatct atcaatgtat gccttatcag agtttctcaa gaggctggag
     2641 tgccggtaat ccgtaaaaag ctcactgaat ttggttttga cacaagcact tggtggcaag
     2701 cggatcgaag tgatggttta caactggcaa tggctgcact tggggagaat atccctgtca
     2761 caatcgaatc gctaataaaa tcttatgcca ttcttgctaa caaaggacac tcatttgaca
     2821 gaggaaatag tgccattatt tcagaaacaa cgaattactc aataaatcat atgcttgaaa
     2881 atgctgttac aaatggcact ggaaaactcg cagttattcc tggtgtttct gtagctggta
     2941 agacaggaac tgtaattgaa aataatgata aatatcttgc tttgtttgcc gggtatgtgc
     3001 cagctgataa tccgcgttat gttgtattag tcgtgattga agagggtcat ttcagtaaaa
     3061 atggaaagac attagttttg ggaggagaat tggccgcacc ggtattccga aatgtcgcaa
     3121 tggatgctct gagcagtgta aatcaataac tagcttatgg gtttgcatat ccatatattc
     3181 aagaatgttg aggttgttat gaagaaaata attatatttt tgtgcacagg attattattt
     3241 tgcgtctcta cttgggctca aggcacctgt tttttagcaa aagaaaatca aactgtttta
     3301 aaacgtgagg gcaatgattg tgatcaacgc tattcgccag catcaacttt taaaattgcc
     3361 cttagcctca tgggctttga ttctggaata ttaaaggatg agttgcatcc tgaatggcca
     3421 tacaaaaaag aatatgaact ttatctgaat gtatggaagt accctcaaaa cccgcatacc
     3481 tggatcagag actcctgtgt ttggtattca caggcattaa ctcgccaatt gggcatgaaa
     3541 cggtttaaag ggtatgtcga cgcatttcac tatggtaatc aggatgtttc gggagacaaa
     3601 ggtcaaaata acggtttaac ccatgcctgg ctttcaagct cactttctat ctcacccaca
     3661 gagcaaattc aatttctaca aaaaataatc tataagaaac taccggtgag tcaaaaagct
     3721 tataccatga ctaaaaacat catgtacatc caggagttgc ctggtgggtg gaagctttat
     3781 ggaaaaacag gaactggtcg acagttaaca aaagataaaa gtcaaaaatt gcctttacaa
     3841 cacggatggt tcgtaggttg gattgaaaag gatgagcgtg tgattacctt tgcaaaacat
     3901 attgcagaca gtaaagagaa taccactttt gccagtttta gagcgaaaaa tgatacattg
     3961 attcaattgt ttaacctgat taatgaactg gagaaataat ctaaaagatg gccggttcag
     4021 tgttttctga tccggctatc taactctctg aattataatc actggttgtt aatttcgcat
     4081 tttagtttgg atgcttgttt attaacccag attaaaaatt taatgagatc attaatttct
     4141 tccgttgaga ggtttttgcc tatcggtggc atgtatcctc gcccattctt tacaagttta
     4201 ctgatatctg aaggcgttaa ataactcatc accataccta agtctggtgc gcgtccttga
     4261 gcccccttgt aaaaaagaga gtggcatgtg gagcatatat tattaaaaat atgatgacca
     4321 ttttgcacag aagcagattc attcaatgga tttaaaaaat gagaaaaagt cagctctttc
     4381 ttatttacta tttgcatcga aataacctgg tatggccaac tcacagggta ggtacttgta
     4441 ttatcccata caatatagaa aggaccagga ttttcttttt taccgtgttg attaatgtaa
     4501 ctccagcgac catctttggt gataatattg tcagagattg ttccgggcgt ttcttgataa
     4561 gcaagatagg catgtccctt tgtcgtaaaa tacaacaagg gagtttgaga cacatattta
     4621 tctattgttt gagctaataa tacctggttt gcattatttt tttgtaaatc tgctcccatc
     4681 aattgctcta aaatctgatt catatcaaac gcatcgtatg tttttttctg ataattatat
     4741 gcaggattat gagagataat cacacgaatg gttttaaatt gtttttttag ctccgacagg
     4801 gtatatgttt tattggcaat agtaatttta agtagttttt ctggtgaatt gggttctcca
     4861 aaacaacatg ttgaaactaa taccaggtga accgcataaa tcagaagatg cattaatttt
     4921 gggctcttgt ccataagtcc ttcttgtttg ttgaaaacaa atcagatttt tcccatactg
     4981 ttactagcta ttatggttta taacaggacc gattttccag cgcgcagagt tatttaactt
     5041 aaatctgaga cacttataa
//
LOCUS       DATNSN010000399         1547 bp    DNA     linear   BCT 25-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000446
            SAMN39051481-rid20645493.guided.306, whole genome shotgun sequence.
ACCESSION   DATNSN010000399 DATNSN010000000
VERSION     DATNSN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39051481
            Sequence Read Archive: SRR27318010
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002037385.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002037385.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/23/2023 03:32:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,266
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,162
            CDSs (with protein)               :: 3,162
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 26 of 54
            Pseudo Genes (incomplete)         :: 19 of 54
            Pseudo Genes (internal stop)      :: 15 of 54
            Pseudo Genes (multiple problems)  :: 6 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39051481-rid20645493.guided.306"
                     /isolate="LP000446"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2019-06"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            16..1017
                     /gene="aph(9)-Ia"
                     /locus_tag="VAH76_003265"
     CDS             16..1017
                     /gene="aph(9)-Ia"
                     /locus_tag="VAH76_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HEO1387825.1"
                     /translation="MLRNNIPDQQIIELLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     KSNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQMDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREMVRSFYSQ
                     IGFDKCDDQITTDFKSFFNQNIDLIHQLVNSSEELSKKIQPDFEKYVLCHSDIHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGGGIGNVWNKPHEIDYFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHELLSRDQNAQSRLESFKHFKSMFDPNDVIEIAFASDRCH"
     gene            1180..1407
                     /locus_tag="VAH76_003266"
     CDS             1180..1407
                     /locus_tag="VAH76_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO1387826.1"
                     /translation="MKKFHVSLLTAMVVSWMNIAYADEQSCNKSYDYCMSSCENQYNQ
                     CTKNNDESYCKEQYQPCIQGCSNTRDSCLSQ"
BASE COUNT          533 a          250 c          268 g          496 t
ORIGIN      
        1 tagtaattga ctcttatgct aagaaataac attccggatc aacaaatcat tgaacttttg
       61 aaagtttatt atggaattga tattcatact gtacaactta tcgtgggtgg tgctgatatg
      121 aatgcctttg gatataaagc agattcaaaa tccaattcct atttcgtaaa gctaaaatac
      181 ggccatcatg atgaaattaa tttatcaatc atccgtctct tgcatgactc tggagtaaag
      241 gaaattgttt ttcctatcaa cacactcgaa gcaaacttat ttcagcaaat ggatcatgtt
      301 aaaataattg tgtatccatt tattgatgcg cccaatggtt tcaccaaaaa tttaacagaa
      361 aaacagtgga aacagcttgg gaaaatatta agacaaatcc atggaacctc cgttcccacc
      421 tcgattcaac agcgattaag aaaagaaact tactcgccta aatggcgtga aatggtaagg
      481 tctttttaca gtcaaattgg atttgataag tgtgatgatc aaattaccac tgacttcaaa
      541 tcttttttta atcaaaatat cgacttaatc catcaattag ttaattcttc agaggagctg
      601 tctaaaaaaa ttcaacctga tttcgaaaaa tatgtacttt gtcattctga catacatgca
      661 ggcaatgtgt tggtcgttga tgaggagtct atttacatta ttgattggga tgagcccatg
      721 ttcgcaccaa aagaacgtga tttgatgttc attggcggtg gcattggtaa tgtatggaat
      781 aaacctcatg aaatcgatta tttttatgaa ggttatggta aaacaagcat cgataaaacc
      841 attttgtctt attacagaca tgagcgaatt gttgaagata tagccgtgta tggacatgaa
      901 ttgctttcac gtgatcaaaa tgctcagtcc agacttgaaa gttttaaaca ttttaagtca
      961 atgtttgatc caaatgatgt tattgaaata gcttttgcat cagatcgttg tcactaaatc
     1021 cgtattacaa ttagaactag ccaatccact cagcaagaat ggaaaacaga tttgatattt
     1081 cctgctacac ttatctaaac aaggaatggg atttaatttt tgactattgg gttaagtcaa
     1141 aatatacatt ttttcagctc aaatgaagga gacgagtcaa tgaaaaaatt tcatgtgtca
     1201 ctgctcaccg ccatggttgt cagttggatg aatatagcct atgcagatga acaaagttgt
     1261 aataaaagtt acgattattg tatgagctca tgcgaaaacc aatataatca atgtactaaa
     1321 aataatgatg aatcttactg taaggagcaa tatcaaccct gtattcaagg ctgcagtaat
     1381 actagagata gctgcttatc tcagtaacac cgaaaggaca aaaatgaaga tatcacaggt
     1441 aagtgttttt ttcttcctaa taattgtttt taatttatct atttttgcgt ctccggaaaa
     1501 caaaataatt tttaatacga atttacaatt atataaactg gcgtcta
//
